LOCUS       DAORNX010000001        30522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_1157_length_30522_cov_5.717924, whole genome shotgun sequence.
ACCESSION   DAORNX010000001 DAORNX010000000
VERSION     DAORNX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 30522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30522
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_30522_cov_5.717924"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..3860
                     /locus_tag="PLO65_00005"
     CDS             279..3860
                     /locus_tag="PLO65_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HRD26660.1"
                     /translation="MFRKRLVATVAAAPLLLLASQALADVTISDKRTAPVATGTVDNG
                     APGNIIISNTGSVKLTTAGPLVTVNSDNTVINNGEISSEDVDNSTGILVVGGTTTTVT
                     NASVIKIVDTFNQTATANDADKDGDLDGAFATGTGRYGIRVTGPGAVTGDILNDTSGS
                     ISVEGNNSWGISVESAVIGDVTNLGAISITGDNVYGMQITGPVTGNVTGRGVVAALGL
                     NAVGVAVDADVSGAVQLQGNITATGYRYSSRPADAEARANLDADDLLQGGPAVRITGD
                     VGAGIMLAVAPGADIDNDNDGWADSYDTDDDNDGILDKDDTDANGDGITDNDYDDDGT
                     ANANDTDDDDDGILDADDPDDNGDGIVDGDTDGDGIVDTSEGSANISVAGGAPALLVG
                     SDTRDITIGAVGTGSLAYGLVIDGTISATGVYDDVEATGVRIGGSGGFATVLTGGLSI
                     DGAISAVSYNANAQGLHLADGADVPSIVVGGSVYAGMAPTSAPLADEDSFNVYAIRID
                     AGANTTSLTNSGSITAFVNGENSNAIAVWDGAGQINSVVNTGTISAVVTATDDADDTD
                     DTDIDPSNETVNGEAVALDLRNAVGGVHIVQYSLTGDTDGDGVLDVTDQDIDGDGLVN
                     GDDTDDDNDGILDVNDTENNLDLDGDGVADSQEASIFGAIKLGAGADVVDLQNGVISG
                     DIAFGAGADEFNVGTAAWKAGYTGEITDSDGQLTLNIVNGVVQLNNTGVLDATSLTLS
                     GDSTLVVTADPAADSATQIQVATANIGDGAQLGLQLTGLISTPSERYTIIRTDTPGGL
                     TVGTIDDSLLGNAPYLVVAEASVDTSVGEVYLDVRRRTATEMSLNDNEAAALDAVYDA
                     LGGDDAVLNAFLGATTRKEFIALYDQMLPDQGEGLFSSLDAITSTISRLTATRPDPRQ
                     RYGPDSFWMQEINVGVMREAGAGLGSDTQAFGFVGGYESMGADGGALGATLAFISAEE
                     KDDVAQIGEETSISLLEAGVYWRRAMGPWLFSARGSGGFAWFDGDRVFISPTDSLIRQ
                     ADSGWNGFTGQASASAAYEANVGVFYLRPLASIDYLYLSEGSRQETGGQDAFNLFIKD
                     RTSSRLSGTAEIAFGATFGRELWWRPEIRFGYRQVLAGEMGETVFRFKNGQLVALKPN
                     ELGDGAAILGLSIKAGTPMSYVAMEAQYEATDGEDRYNLQLAGRMMF"
     gene            3988..4683
                     /locus_tag="PLO65_00010"
     CDS             3988..4683
                     /locus_tag="PLO65_00010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD26661.1"
                     /translation="MSMHPSPPRGSARAVPDDAHGARTEDLRQALAQKTALLHEIDHR
                     VKNNLQLVSSLLQLQARRADDPAVRHALRAAQTRINAVAIVHRRLFQGEDAQAFDVAA
                     FLRDMVDDAIGASGRADIRARLDLQPAELPAAQAAPLALLVSELLTNIVLHAFPGGVG
                     GVVTVTLARDGERLRIEIADNGVGVATTGVSPGIGQTIVGHLCDQLRAESRSTDACPG
                     VRTVLRLPLNGAR"
     gene            4688..5161
                     /locus_tag="PLO65_00015"
     CDS             4688..5161
                     /locus_tag="PLO65_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD26662.1"
                     /translation="MEQSIEESLNVLIVEDEVLVAMELGVLVCEVGCRDVGHAMSSAD
                     AVAMAASLRPDLALVDVHLRDGPTGIEVARRIASDCGVVALFMTANVKRLPEDFAGAC
                     GVIGKPYSEHGVKAALRYLDACIRAGRAPGLPPPGLQLAPAWATRWGLDDLPRTA"
     gene            5161..5427
                     /locus_tag="PLO65_00020"
     CDS             5161..5427
                     /locus_tag="PLO65_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRD26663.1"
                     /translation="MSPLWLNALGTGAALCSMASFTPQILKIWRERDAASVSLRMWLL
                     TVTGFVLWTGYGVALGSWPVAVSNAVCLILSALVLALKWRFSRA"
     gene            5502..6239
                     /locus_tag="PLO65_00025"
     CDS             5502..6239
                     /locus_tag="PLO65_00025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD26664.1"
                     /translation="MIERQLDIRTADGATTTFIVHPERDGPHPVILFFMDAPAIREEL
                     RDMARRLATSGYYVMLPNLYYRAGVMELGPLSPDPDDPGRARMVALMNSLSIPLVMDD
                     AAALLAFAEADPAAWTRSVGAVGYCMSGPYAIAAAARFRDRVRAAASIHGVSLMTDRP
                     DSPHLLARQTDAELYFACAQIDRWFPEEKIAPLRDDLAANGVRAEVELCPGVEHGFVF
                     PQRPAYHKAAAERHWERILALFARRLR"
     gene            6370..6789
                     /locus_tag="PLO65_00030"
     CDS             6370..6789
                     /locus_tag="PLO65_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD26665.1"
                     /translation="MRFMMLMIPGGYETAGPELDLPVDAVEKMMAYNQSLVDAGILRN
                     AEGLHPPATGKRVSFAGGKPLITDGPFAEAKEALGGFWIIEVASLDEAAAWAARCPAQ
                     ENEVIEIRRIQEMEDFSPEVQDVAKGLDLEGSGWSRS"
     gene            7096..7323
                     /locus_tag="PLO65_00035"
     CDS             7096..7323
                     /locus_tag="PLO65_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26666.1"
                     /translation="MSTVQRAYALARSGQFRDVDVLKDRLKAEGCRAVDALLAARSIR
                     KHLEAICAATFKPPLEAPASAEPPNPPTPTE"
     gene            7357..7716
                     /locus_tag="PLO65_00040"
     CDS             7357..7716
                     /locus_tag="PLO65_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HRD26667.1"
                     /translation="MNAIPKAGFADRLRAASAAKQAMLAKMKPRAAVTDPLHAERPAM
                     RAAELERVRAERAEAKAAVKQAQADAEEAARLAEVARAEAELDARRGQRKERKALTKA
                     EQKAARDAKYAARKARQ"
     gene            7744..7893
                     /locus_tag="PLO65_00045"
     CDS             7744..7893
                     /locus_tag="PLO65_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26668.1"
                     /translation="MARKPNYNFERSERERLKAAKAAEKAEAKRRARESGQREEPAPA
                     TEDDE"
     gene            7902..8165
                     /locus_tag="PLO65_00050"
     CDS             7902..8165
                     /locus_tag="PLO65_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26669.1"
                     /translation="MQTATIFNLETVPLDGEVFADCEFRQCRLVYSGGKPPVFDNCRF
                     DACDWRFDDAAARTLAHLKAVWAAGGKAQVQALIKEITVSGGR"
     gene            complement(8192..8767)
                     /locus_tag="PLO65_00055"
     CDS             complement(8192..8767)
                     /locus_tag="PLO65_00055"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD26670.1"
                     /translation="MAANFLSPEDCARLIAMIDADVRPSTSLSASARPTHRTSETCLL
                     PADHPLIAEVETRIADLLGIAASHSETLQGQRYMPGQQFKMHNDYFAAGQPYSETVAQ
                     EGGQRTWTAMVYLNAPATGGHTRFPRAGVEIAPRIGALVTWNNLDGQGLPNPYSHHEG
                     AVVEAGAKYVLTKWFRERTWTRSAGSDALRR"
     gene            complement(9194..10066)
                     /locus_tag="PLO65_00060"
     CDS             complement(9194..10066)
                     /locus_tag="PLO65_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD26671.1"
                     /translation="MLTWQVGAVKVTRVVELEIPVPYSPRRPMIAEATPEALRQTPWL
                     YPDFVNEQDQLLLSIHALLVEAPGLRLVVDTCIGNDRPRAITGGQPLATTFLEAMAEA
                     GWSRERVDAVICTHLHVDHVGWNTMLVDGAWVPTFPNARYLLGRVEFEHWSAEAGEEN
                     QTILDDSVRPVIDAGLAEFVDMDHRLSPEVRLIPTTGHTPGHVSVVIESEGQTAVITG
                     DMSHHPCQMAHPHWSPSFDSDPKASAVTRARMFAEWADQPILVIGTHYAAPTAGHVKR
                     EGEAFRFEGFRSRS"
     gene            complement(10102..10800)
                     /locus_tag="PLO65_00065"
     CDS             complement(10102..10800)
                     /locus_tag="PLO65_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26672.1"
                     /translation="MSLDELARRIADRASPGAVLVVGVTGSVAAGKSTLCAGLKAILE
                     PARRVEVVSTDGFLFPNAVLAERGTLMRKGWPETYDAASLAAVLAAAREGPVTVPGYS
                     HVIYDIDPSLAREVARPDILIVEGLGLTPRPAGLDLLVYLDAEEADLEDWFAARFIDF
                     WRAAEADPASFYARFRSMSEAEADAFARATVWTQMNLPNLRRHIIRARDEADIVVRKR
                     ADHSLWLAGGAGAS"
     gene            complement(10806..11495)
                     /locus_tag="PLO65_00070"
     CDS             complement(10806..11495)
                     /locus_tag="PLO65_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD26673.1"
                     /translation="MSQQPSGIHHLAFMAGDIKKHIAFFAEVMGCPLVALFDMHGVPG
                     GLHAFLKMNEHSYFSIVQLPDVDKIPAQLGVTHAGSGALPSAAGTLQHLAFRADTEED
                     LIGLRDRIRSHGINVIGPIDHGMCKSIYFAGPDHMTLEVATPGNRIDPDLWIDPAVLA
                     KAGIDAAEAARFKAPAPYTGPSPVPQPAYDASKPHMAYPKEAYLKMLAIPDEMLTAGA
                     SYTETPVKAHA"
     gene            11591..12739
                     /locus_tag="PLO65_00075"
     CDS             11591..12739
                     /locus_tag="PLO65_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD26674.1"
                     /translation="MSIIREPYGFAYAEPSRLKETYGGPEGRVFVECMRIRPAEGESK
                     TAILFSHPIGGGSFLPLVTALAHAGRHVIYCNPRYRGNDTALILEKCVTDLGACIADL
                     KKRFGYEKVVLGGWSGGGSLSLFYQEQAVHPTITETPAGDPVDLTAAGLQPADGIMLL
                     AAHISRSVTMTEWMDPSILDEDKPFERDVELNIYDPACPNQPPYSPEFVARFREAQIA
                     RNRRITARVKATLAELKKTDPDMERPFVVYGTMSDVRWTDPTQDPSDRKPHTCYLGDP
                     RIVNDGPVGLGRFTTLRSWLSQWSYDESRAHGLKNAANVTCPTLVISNTADLACTPSH
                     AARLFEALASPDKTFVEVKGADHYYIERYDLLPEAVAAVTGWLDARGF"
     gene            complement(12885..13883)
                     /locus_tag="PLO65_00080"
     CDS             complement(12885..13883)
                     /locus_tag="PLO65_00080"
                     /inference="COORDINATES: protein motif:HMM:NF027488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HRD26675.1"
                     /translation="MALVDDGYTALADLISAFDEAEQVGCHVFLKSKPTRRRRDPWHM
                     LSMAGNLADEMVETAETTLREIAARAADEDLIEEFTFDAMADHDIGVLSVADTPGLGQ
                     WLEEVPVPDWPQRFQGDEDVLENTRLYVTRLSFPDGRVLLNFRGSRNLNVALRRRNAV
                     IAQFRPDADELVPVGGTVVNFDQDVDFFLWEGLVFVRNLRTFESVTNIRRVTAQKAGE
                     AVDALGERFALDDAEGLKEAIGARPLLAKKLASALRHGLVADIDVGALPERIAAKELD
                     IVCEADGEGFRLRIDARDRRQVQDFVDLMSDVFLHSPVTGREWVVSVKKPPRPRRR"
     gene            complement(13896..14408)
                     /locus_tag="PLO65_00085"
     CDS             complement(13896..14408)
                     /locus_tag="PLO65_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26676.1"
                     /translation="MTNFFRLQFVFSSFGPLFALMAVVFVVQNRPWSAAVAGLLALCA
                     FVTFLALATGMKRKSPSFEVVRVDARLDQHVLSHLISYLPPVLMDDFGTVETIVPVFA
                     FYAIIVLLMLKADSIYVNPFFLWCGYRVHQVRLESDRSVVIVTRRTSTITGDRLALYE
                     VDATRLYFAE"
     gene            complement(14781..15428)
                     /locus_tag="PLO65_00090"
     CDS             complement(14781..15428)
                     /locus_tag="PLO65_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26677.1"
                     /translation="MLTVVIGAVPGTDAIGPTWKGFGKALRNVMEFQRRRSEAWRTVQ
                     AIAWLEVDAVLGADVPRLKPIKTAQFAEIGPLSYRLNDPVWWVTCHGLIDSGEVGLDR
                     VERALKDHWHGTRQLHLVRLHRNRTKEANIKQVASYALKHRHKTELADRTMTWPIEWV
                     AEYHNWLGSWSRGFQSVKLSINPSRFDIVDRPVVISEEVEPMPFEHSVSTFTMIY"
     gene            15655..16098
                     /locus_tag="PLO65_00095"
     CDS             15655..16098
                     /locus_tag="PLO65_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26678.1"
                     /translation="MTVFSFGATNAPGGASHGPGERRITPKWTFGERRWHRHGMTNRH
                     VPVVTDGSPSAFLDRLPHLAGLSVFHLPSLRTVEDLVTWQAEGPELMGEVAEWCQDTN
                     WTGQVWINRSDAPAMMSVAIEASVDAVMFALRWSDLIGSQHRYAA"
     gene            complement(16086..16661)
                     /locus_tag="PLO65_00100"
     CDS             complement(16086..16661)
                     /locus_tag="PLO65_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRD26679.1"
                     /translation="MRIGYARVSLSSQDTKMQIDALKAAGCDHIYEEKMSGRIDDRPE
                     FLKALAFAREGDEIVVYALSRLARNMKQLLATTDDLQRRGIGLRSLTEAIDTTAGGPS
                     GSLLLNLMAAINQFEVDCLRARTKAALAARGPGQRGGRPKVMTEPKIAMAKALLIEGK
                     LPVAEVAREIGVGVSTLYRALPQAKALAHAA"
     gene            complement(16828..17292)
                     /locus_tag="PLO65_00105"
     CDS             complement(16828..17292)
                     /locus_tag="PLO65_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26680.1"
                     /translation="MVAVGERSVVTVIRTIAGSAMVRKEMSRIRDRWFHAEMEDWAVI
                     RFAGCNIVLQPAEWNDWSYIGATWLFLLPGAAVWWDVVLDRATGQLFQVELPDADDQE
                     GSAAMSEFNKWAFHVLARLNGLDEVRVRRPSPRQQREALERAEGILPRLPFY"
     gene            complement(17322..18437)
                     /locus_tag="PLO65_00110"
     CDS             complement(17322..18437)
                     /locus_tag="PLO65_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26681.1"
                     /translation="MDDDNPLGTETLPGGRALFPVHSGWALDQVVQIDDAAPGFLARV
                     CRARLQWRQALWACLAAAGATSGEVVFRLTGDDGWLTASRRPVIAEFAAVAPKASARD
                     LIEASYRSCPDGLLGALRKLHGQPFGLPDFYGLLHRLFASDDPLDRRRRAALEQCGTL
                     DECRLEAVLAMSDPGLLVPGVVNGLDGEEAVRRFERDVAIIRRLCSWATDDAIKEAVT
                     QARERRSRPFLAAMLSRADRLFPETHPCDADPDLERFPIAKAREVGQSFRNCLSPERI
                     LPQAASGVWAMILWRGAELLIETRLLDSGAWAVHRVHAAGNQRVERQTLLRVRDRLAP
                     LGVVCPVCVEPGDELAGVADAFGGWDGIALVAFDAWD"
     gene            complement(18578..19645)
                     /locus_tag="PLO65_00115"
     CDS             complement(18578..19645)
                     /locus_tag="PLO65_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26682.1"
                     /translation="MPPKDIRAVLGRMIPEDERLRLRAELLKSYKAQHLPGAQCALCA
                     QEHRPLALHVCDFYEDASCPPRTPALLIGKNSIGRRWSLPVCTECAPPCPKCGLPIVS
                     GWIKKSVEALQAVPWARKAKIGLGICRHFHPLRDIASRAKKPYWTPESLSAETEETRR
                     GVVEGAADRRHTKAEPRPSVPARKPSSSSDARAALERQFAGWDVGEFGGMTAFTRYHE
                     QPSGFRLRVSLIPAAGHRINLFAALIPPPDELNTDRQEAIDSVGKVEIAVGSSSVALV
                     EGELSLLGQHGVQLTGWFVFTDFLQKLSEADPRSGEIAIFLRTSEPFQSRLGGRCVTV
                     PTDGLFETIRAVGEQIARNRG"
     gene            complement(19732..20949)
                     /locus_tag="PLO65_00120"
     CDS             complement(19732..20949)
                     /locus_tag="PLO65_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD26683.1"
                     /translation="MADLSLDDLRAELADFAPAEAKAATYTPRQERIIAGFEDIVRFY
                     EEHGRAPLHGEDRDIFERLYAVRLDRLRDQEECRSLLAPLDKHRLLDASPKTVEPEEM
                     DLEALRAELAGSDAQDITQLRHVPSREERRAAEEIANRERCEDFDKFEPLFKQVQQDL
                     DAGRRSTRPFVRDAGFQKADIRAGEFFILGGQIAYVAAVGEPIKAPNGETDARLRVIY
                     SNGTESDLLLRSLQRALYKDEAGRRITEPEAGPLFAGVSDGPAPIFASEAEDGDVETG
                     TIYVLRSKSDHPFVAEHRDVIHKIGVTGGTVEARLAGADKSATYLLADVEVVATYKVF
                     GVNCRQLEGLLHKVFAPALLDLTLTDRFGNPVKPREWFLLPIAAIDEAVEHIRDGSIV
                     GLRYDPGTGRLIA"
     gene            complement(20942..>22754)
                     /locus_tag="PLO65_00125"
     CDS             complement(20942..>22754)
                     /locus_tag="PLO65_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD26684.1"
                     /translation="ADEPLSKFGFEADWTVKPQWNLCNAPGPDEERVDSSKVKAVGQF
                     LASDDKVLVCTHATFRFAVDQLGVEAFDDRLIAVDEFHHVSANPDNRLGSQLGEFIKR
                     DKAHIVAMTGSYFRGDAAPVLMPEDENKFETVTYTYYEQLNGYEHLKTLNIGYFFYTG
                     RYLSAIEAVLDPARKTIVHIPSVNSRESTADKIKEVEHIIQSLGEWIEADPVTGFHRV
                     RLKGGGRIIKIADLVDDSDQAKRAKVLTALKDPAQRNNRDHVDVIIALGMAKEGFDWI
                     WCEHALTVGYRSSLTEIIQIIGRATRDAPGKESASFTNLIAEPDASEAAVVDAINDTL
                     KAIAASLLMEQVLAPRFNFTPKDHGPVEGYDYGEEGYKPGQTNVGVNETTGQLHFEIA
                     GLVEPKSPEAARVCREDLNEVITAFVQDKTALERGLFDEETVPEELTQVRMGKIVRDK
                     YPDMSPEDQEAIRQHAIAALNLTQKAKELASGGDSATGEPKANTALLDGVRKFAMDVR
                     DLDIDLIDRINPFEAAYAVLAKSMNEATLKQVQSVISAKRTTLSPEEAKDYAARAVRF
                     RRERGRPPSIDAADEWEKKLAEGAAAFMRYDREGRYG"
     assembly_gap    22755..22967
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23023..25791)
                     /locus_tag="PLO65_00130"
     CDS             complement(23023..25791)
                     /locus_tag="PLO65_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HRD26685.1"
                     /translation="MNPVEIEEAVSNLALEPFDRAEFPLQFLRAFDNPETTIKRLRSG
                     STNQSDIPGGVLQRNNIHIVTCAPGEVDSTLKALRESPKTASAKAKFILATDGEAFQA
                     EDLNGGGTVACSYVEFPDHFGFFLPLAGITTVREIRESSFDVKATGRLNRLYVQLLKE
                     NPDWAKADRQHDMNHFMARLIFCFFAEDTDIFLEEGLFTKTVEQMSAGDAASTHRVIG
                     EIFRAMDTKPEDREAASLPRYATKFPYVNGQLFSGSTDVPTFSKVARSYLLHIGSLNW
                     KKINPDIFGSMIQAVTDDEERGSLGMHYTSVPNILKVLNPLFLDDLRQQLEEAGTNGR
                     KLLNLRNRISRIRVFDPACGSGNFLVIAYKEMRGIEAEINKRRSEPDRRSDIPLTNFR
                     GIELRDFPAEIARLALIIAEYQCDVLCRGQKEAVADFLPLGSENWITCGNALRLDWLN
                     ICPPTGTGVKVTSDDLFQTPLDQAEIDFENEGGETYICGNPPYLGSTWQSAEQKADLE
                     RAVARHVRSWKTLDYVAAWFIKAAEYGTHTPSAAAFVATNSICQGQQVPLLWPAIFNL
                     GHSICFAHSSFKWANLASNKAGVTVVIVGIGRECGSSRLLYDDVPNGDTTLRRVENIN
                     AYLVPGPDVFVEKSSRPLNDLPRMSFGNKPVDGGHLLLTVSEAQSLGLTDTQRRKFLR
                     KNYGGAEFIRGIERYCLWISDQDLAEAAAVPALAERFAAVKAMRLKSPDPAANTMAAR
                     SHQMREMMIGQRHTVVAAAISSENREYLPCGLIDSNATINNKNYGIYDGPLWSLALLA
                     SRLHWVWIGAVCVRLEMRFSYSNTIGWNTFPVPNLTSQNKSDLIRRAEEILLAREAHF
                     PASIADLYDPEKMPEDLRHAHEANDEVLERIYIGRRFRNDTERLEKLFELYTKMTTKP
                     GGPKKAAA"
     gene            25857..26675
                     /locus_tag="PLO65_00135"
     CDS             25857..26675
                     /locus_tag="PLO65_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD26686.1"
                     /translation="MLSFKPLLQSAGFDPKEVNLLRHCPGGITDPFGAWRADRAAFEA
                     YQSMQARRDRSYFSRPYWASFAPTRQGRTIFLGIYEARLIGPVIEGEIDALTGEVLRE
                     ERADRYDCKLRPELSDYIGRLVIDWGAGTRSWGQKGTTDKPIIELLRGVEEPRFPGYL
                     ELIEPLSAVPTFPERWVDFLREGRGVYLLTCPRTKEQYVGKADSADGFWGRWMAYATT
                     GDGGNVRLRSRERSDYQVSILEVAGSAASPDDILRMEVRWKAKLQTREMGLNGN"
     gene            26702..27496
                     /locus_tag="PLO65_00140"
     CDS             26702..27496
                     /locus_tag="PLO65_00140"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HRD26687.1"
                     /translation="MTAIILAPTPEVRAACDRIDDAISRALESLGRTITAHQTWGTYE
                     SSRDAVALFKASVRSVESTIALARTDLVLFPGALMTARGAFEISLRAAWLVDCDDPFD
                     RERRWLAHLHNEENSNGKVAAHLKGAGGDPESFLAMQEQMRTFRAAIEAKFPPGYAVV
                     PKVPNVPEMVRTLHEEPVYILYCYLSQFIHGARRAANYYATDGSPRRDAVTPKDWYLP
                     LAVCWISLFRGARKLLERLDGRPDDFMTIEESTEIEGAIAAVGATA"
     gene            complement(27542..28960)
                     /locus_tag="PLO65_00145"
     CDS             complement(27542..28960)
                     /locus_tag="PLO65_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD26688.1"
                     /translation="MAAFGRESRITQAFGRSGQTESPDEFMERMAAEERQVRAWIAEI
                     VRAEGWSLPPEENPVPNPLHQTLYMAVLTAWKAILAKERRWLNNDFADLPTAPPPTRQ
                     AAEPPLGRPTSLTLQQAFAAWSGMSAARSARTRQHRTVIDAKLAVRRFVELHGDLPLS
                     EISRRQAREFRDKFAQVPARLPMRLANTPLPRLLEEDLSAYPARTATTINKSLNLLAA
                     IASHAEREGDLDAFPAWKNPFGVQLVIDPQTTGSYEPFSAKDLNRLLSSPVFSRGERP
                     QRGRKEAAKWLPLLAMLHGARRGELVPLRVRDVVADDETGIRYLQIAPELESGRRVKN
                     LGSIRKTPLHPKAVELGFLDYLDRRREAVGPTGLLWDGLDTDSRVTRWTEWFHRYLRE
                     AGVDHPRKRFHSFRHTFKRVARDTLDAAVIDALCGHVSGVGGGYGKARDGARKDSGYN
                     LTALAKAMEKIEFRGVDFSVVR"
     gene            complement(29392..29468)
                     /locus_tag="PLO65_00150"
     tRNA            complement(29392..29468)
                     /locus_tag="PLO65_00150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29432..29434),aa:Arg,seq:acg)
     gene            complement(29567..>30522)
                     /locus_tag="PLO65_00155"
     CDS             complement(29567..>30522)
                     /locus_tag="PLO65_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26689.1"
                     /translation="GAGPSLPLLTPRQVTAPATREEPGMPQSYEKPRGRMLIYWGCGE
                     RARPGQPVVIDFAKVADGQVPPAFTQMMQGLQVSAMQPPSPTRSRTYGEWPNDRARTQ
                     VPPDGSLTGTHTIRGNYSPQIQFSMTPDQDFLPPVQMTTNSPAATGGVQLGWRPVDGA
                     VGYMATTMGGDGETVVVWSSSEVSGLLFALPDYLKPSDVTRLLASKALMAPSQTSCAV
                     PKEVVAAAPQSMYQFVAYGDEANFSYPERPADPRQAWNIAWTVKVRYRSATGGMLGME
                     MPGAYGGDQAATPGKPKPAKPARPRATDILRGLGAAIPGGR"
BASE COUNT         5278 a        10047 c         9775 g         5209 t
ORIGIN      
        1 gcctgtccgg ccatggccag cgccagcggc gtggcggccg cggccaggcc ggccagcagg
       61 cgggggcgca gcgtcgtgac ctcgtcctcg gggggcatgg cgcgaaagcc tcgattccat
      121 tttttcggca ggctaaggta gggggagata tggcgccctt gcaggccttg cgatagacga
      181 ggcggggtcc ctggggatgg ttcatgacgg ctgacgcaat ttcgccgcat cgaaagggga
      241 cgaacgtgtc gccgttaaag gtccggggtt ctctgttcat gttccgcaag cgtctggtcg
      301 ccaccgtcgc cgccgccccc ctgctgctcc tcgcgagcca ggcgctggcg gacgtcacga
      361 tctcggacaa gagaacggcg cccgtcgcca ccgggaccgt cgacaacggc gcccccggca
      421 acatcatcat cagcaacacc ggcagcgtga agctgacgac cgccggcccg ctggtcacgg
      481 tcaacagcga caacaccgtg atcaacaacg gcgagatctc ttcggaagac gtcgacaact
      541 cgacgggcat cctggtcgtt ggcgggacca cgacgaccgt gaccaacgcc agcgtcatca
      601 agatcgtcga caccttcaac cagaccgcca cggccaacga cgccgacaag gacggcgacc
      661 tcgacggggc cttcgccacc gggaccgggc gctacggcat ccgggtcacc gggccgggcg
      721 cggtcacggg cgacatcctc aacgacacca gcggctcgat ctcggtcgag ggcaacaact
      781 cctggggcat cagcgtcgaa agcgccgtga tcggcgacgt gacgaacctc ggggccatct
      841 ccatcaccgg cgacaacgtc tacggcatgc agatcaccgg tcccgtgacg ggcaacgtca
      901 ccggccgggg cgtcgtcgcg gccctcggac tcaacgccgt cggggtggcg gtcgacgccg
      961 acgtctcggg cgccgtccag ctgcagggca acatcaccgc caccggatat cgctattcga
     1021 gccgtcccgc cgacgccgag gcgcgcgcaa acctcgacgc cgacgacctg ctgcagggcg
     1081 gacccgccgt gcggatcacc ggcgacgtcg gcgcggggat catgctggcc gtggccccgg
     1141 gcgcggacat cgacaatgac aacgacggct gggccgacag ctacgacacc gacgacgaca
     1201 acgacggcat cctcgacaag gatgacaccg acgccaacgg cgacgggatc accgacaacg
     1261 actacgacga cgacggcacg gccaacgcca acgacaccga cgacgacgac gacggcatcc
     1321 tcgacgccga cgaccccgac gacaatggcg acggcatcgt cgatggcgac accgatggcg
     1381 acggcatcgt cgacaccagc gaaggcagcg ccaacatctc ggtcgccggc ggcgccccgg
     1441 ccctgctggt cgggtccgac acgcgggaca tcaccatcgg cgcggtcgga acgggcagcc
     1501 tggcctacgg cctggtgatc gacggcacga tcagcgccac cggagtctat gacgacgtcg
     1561 aggccaccgg tgtccgcatc ggcgggtcgg gcggcttcgc cacggtgctg accggcggcc
     1621 tgtcgatcga cggcgccatt tcggccgtgt cctacaacgc caacgcccag ggcctccatc
     1681 tggcggacgg ggccgatgtt ccatcgatcg tcgtcggcgg ttcggtctat gccggcatgg
     1741 cgccgacctc ggcgcctctg gcggacgaag actcgttcaa tgtctacgcc atccgcatcg
     1801 acgcgggggc caacaccacg tcgctgacca actccggatc gatcaccgcc ttcgtcaacg
     1861 gcgagaacag caatgcgatc gccgtctggg acggcgcggg ccagatcaac tcggtggtca
     1921 acaccgggac catctcggcc gtggtcaccg ccaccgacga cgcggacgac acggacgaca
     1981 ccgacatcga ccccagcaac gagacggtca acggcgaggc cgtcgccctc gacctgcgca
     2041 acgccgtcgg cggcgtccac atcgtgcaat actcgctgac aggcgacacg gacggcgatg
     2101 gcgtgctcga cgtgaccgac caggacatcg acggcgacgg tctggtcaac ggcgatgaca
     2161 ccgacgacga caacgacggc atcctcgacg tcaacgacac cgagaacaat ctcgacctcg
     2221 acggcgacgg cgtcgccgac tcgcaggagg cctcgatctt cggcgccatc aagcttggcg
     2281 ccggcgccga cgtggtcgac ctgcagaacg gcgtcatctc cggcgacatc gccttcgggg
     2341 ccggcgccga cgagttcaat gtcggcacgg ccgcctggaa ggccggctac accggcgaga
     2401 tcaccgactc cgacggccag ctgacgctga acatcgtcaa cggcgtggtg cagctgaaca
     2461 acaccggcgt gcttgacgcc accagcctga cactgtcggg cgacagcacc ctggtggtca
     2521 ccgccgaccc ggccgccgac tcggccaccc agatccaggt cgcgaccgcc aacatcggcg
     2581 acggggccca actgggcctg cagctgacgg gtctgatctc gacgccgtcg gaacgctaca
     2641 cgatcatcag gaccgacacc cccggcgggc tgacggtggg aaccatcgac gacagcctgc
     2701 ttggcaacgc gccctatctg gtcgtggccg aagcctcggt ggatacctcg gtcggcgaag
     2761 tctatctgga cgtccgtcgg cggacggcca ccgagatgag cctcaacgac aacgaggcgg
     2821 cggccctcga cgcggtctac gacgcgctcg gcggtgacga cgcggtgctc aacgccttcc
     2881 tcggcgccac gacccgcaag gagttcatcg ccctctacga ccagatgctg cccgaccagg
     2941 gcgagggcct gttctcgtcg ctcgatgcga tcaccagcac catttcgcga ctgaccgcga
     3001 cccggcccga tccgcgccag cgctacggtc ccgacagctt ctggatgcag gagatcaatg
     3061 tcggcgtgat gcgcgaggcc ggcgcggggc tcggatccga cacccaggcg ttcgggttcg
     3121 tcggcggcta cgagagcatg ggcgccgacg gcggggccct gggggccacg ctggccttca
     3181 tcagcgccga ggaaaaggac gatgtggccc agatcggcga ggagacctcg atctccctgc
     3241 tcgaggccgg cgtctactgg cgcagggcga tgggcccctg gctgttcagc gcccgcggca
     3301 gcggcggctt cgcctggttc gacggcgacc gggtgttcat ctcgccgacc gacagcctga
     3361 tccgccaggc cgactcgggc tggaacggct tcaccggcca ggccagcgcc agcgccgcct
     3421 atgaggccaa tgtcggcgtc ttctacctgc ggccgctggc cagcatcgac tacctctacc
     3481 tcagcgaggg gtcgcgccag gagaccggcg gtcaggacgc gttcaacctg ttcatcaagg
     3541 accgcaccag cagccgcctg tcgggcaccg ccgagatcgc cttcggcgcc accttcggcc
     3601 gcgagctgtg gtggcgaccg gagatccggt tcggctaccg gcaggttctc gccggcgaga
     3661 tgggcgagac cgtgttccgg ttcaagaacg gccagctggt cgccctgaag cccaatgaac
     3721 tcggggacgg cgccgccatc ctcggcctgt cgatcaaggc cggcacgccg atgtcctacg
     3781 tcgccatgga ggcccagtac gaggccaccg acggcgagga ccgctacaac ctgcagctcg
     3841 ccggccggat gatgttctag gcccgcccgt cgaccttccc ggggaggtcg acggccgcgc
     3901 cgcggctcgc cgagcgcgcg gcgcggtgga actgcccgac gtttccaccg ttttgagccg
     3961 gcgtctatgg gccggagttc gagcggcatg agcatgcatc cgtcgccccc ccggggttcg
     4021 gccagggcgg ttccggacga tgcccacggg gcgcgcacgg aagacctgcg acaggcgctg
     4081 gcgcagaaga ccgccctgct ccacgagatc gaccaccggg tgaagaacaa tcttcagctg
     4141 gtctcctcgt tgctgcaact gcaggcgcgg cgggccgacg accccgcggt gcgccatgcg
     4201 ctcagggcgg cccagacgcg gatcaacgcc gtcgccatcg tccatcgccg actgttccag
     4261 ggcgaagacg cccaggcctt cgatgtcgcg gcctttctgc gggatatggt tgacgacgcc
     4321 atcggcgcct cgggccgggc ggatatccgc gcgcggctcg acctgcagcc ggccgagctg
     4381 cctgccgccc aggccgctcc cctggccctg cttgtcagcg aactgctgac caacatcgtc
     4441 ctgcacgcct ttccgggcgg ggtcggtggt gtcgtgacgg tcacactggc cagggacggt
     4501 gaacggctaa ggatcgaaat cgccgacaac ggcgttggag tcgcgacgac cggagtcagt
     4561 cccggcatcg gccagacgat cgtcggacat ctgtgcgacc agcttcgggc cgagtcccgc
     4621 tcgaccgacg cctgtcccgg agtccggacg gtccttcgtc tgccgctgaa cggtgcgcgt
     4681 tgaagttatg gaacagtcga tagaggaatc cctgaacgtc ctgatcgtcg aggacgaggt
     4741 gctggtggcg atggaactcg gcgtcctcgt ctgcgaggtc ggctgccgcg acgtcgggca
     4801 cgccatgagt tccgccgacg ccgtcgccat ggcggccagc ctgcggccgg atctggccct
     4861 ggtcgatgtg cacctgcggg acggtccgac cgggatcgag gtggcccgcc gcatcgcctc
     4921 cgactgcggg gtggtggcgc tgttcatgac cgccaacgtc aagcggctgc cggaggattt
     4981 cgccggcgcc tgcggcgtga tcggcaaacc gtattccgag catggcgtga aggcggccct
     5041 gcgctatctc gacgcctgca tccgggccgg ccgggcgccc ggactgccgc cgccggggct
     5101 gcagctggct ccggcctggg cgacccggtg gggcctggac gacttgcccc ggacggcctg
     5161 atgtcgcctc tgtggctcaa cgccctgggg accggggcgg cgctctgttc gatggccagc
     5221 ttcacgcccc agattctcaa gatctggcgc gagcgcgacg ccgcgtcggt ctcgctgcgg
     5281 atgtggctgt tgacggtgac cggcttcgtg ctctggaccg gctacggcgt ggccctcggc
     5341 agctggcccg tggcggtgtc gaatgcggtc tgcctgatcc tcagcgccct ggtgctggcc
     5401 ctgaagtggc gattcagccg ggcttaacca cgcctcgaga tccgttgctt gatccgtcgc
     5461 cggtctttcc tccatcgtgc gacgatgtac cggggatccc catgatcgaa cgccagctcg
     5521 acatccgcac cgcagacggc gcgacgacca ccttcatcgt ccatcccgaa cgcgacggac
     5581 cccatccggt gatcctgttc ttcatggacg cgccggcgat ccgcgaggag ctgcgcgata
     5641 tggcgcggcg gctggcgacc agcggctact acgtcatgct gcccaatctc tactaccggg
     5701 ccggggtgat ggagctgggg ccgttgtcgc ccgatcccga cgatcccgga cgggcgcgga
     5761 tggtcgccct gatgaacagc ctgtcgatcc ccctggtgat ggatgacgcc gcggccctgc
     5821 tggccttcgc cgaggccgac cctgccgcct ggacccgctc cgtcggcgcg gtcggctatt
     5881 gcatgagcgg gccctacgcc atcgccgccg ctgcccgttt ccgcgaccgg gtcagggccg
     5941 ccgcgtcgat tcacggcgtc tcgctgatga ctgaccggcc cgacagcccg catctgctgg
     6001 cccgtcagac cgatgccgag ctctatttcg cctgcgcgca gatcgaccgc tggttccccg
     6061 aagagaaaat cgcgccgctg cgcgacgacc tggccgcgaa cggggtccgg gcggaggtcg
     6121 agctctgccc cggcgtcgag catggcttcg tgtttcccca gcgcccggcc taccacaagg
     6181 ctgccgccga gcggcactgg gagcggattc tggccctctt cgcccgccgc ctgaggtagc
     6241 ggcgacggcg cgctcgatcc gggatcacgg tgatttcgag acccggcgga tcgctcgacg
     6301 aatatgtcgg gatcacacct cgccatccgt cattctgacg aacaccggac ccacagagga
     6361 accacgtcca tgcgtttcat gatgctgatg atcccgggcg gctacgaaac ggccggcccc
     6421 gaactcgacc tgcccgtcga cgccgtcgag aagatgatgg cctacaacca gtcgctggtt
     6481 gacgccggca tcctgcgcaa tgccgagggc ctgcacccgc cggcgaccgg caagcgggtg
     6541 tcgttcgcgg gcggcaagcc gttgatcacc gacgggccgt tcgccgaggc caaggaggcc
     6601 ctgggcggct tctggatcat cgaggtggcg tcgctggacg aagcggccgc ctgggccgcc
     6661 cgctgcccgg cccaggagaa cgaggtcatc gagatccgcc ggatccagga gatggaagac
     6721 ttctcgcccg aggtccagga cgtcgccaag ggcctcgacc ttgagggcag tggctggtcc
     6781 cggtcctgat atcctcctgg aggaaggcgg agagcggcgc gcgccgtcct cgatcgcgcg
     6841 cggccgcggc cttcaaatca ttgatgaatc ggagtgcttc ccgccggcgc ggaggttgat
     6901 cggggatcga caggctgtat ttcattggcg tctgacccct gtttcgcgta tgggatcgtt
     6961 gtcgaatatc gttctggttc ggttgctctc tacgcttggt cttcggatcg ggttccgggc
     7021 cttccgcgtt cctttccgac aatttgatcg cgctgcgtcc gatcggatcc gcgcgtcctc
     7081 aagcagatca agaccatgtc cacggttcaa cgcgcctacg ccctggcccg gtccggccag
     7141 tttcgtgatg tcgatgtcct caaggaccgg ctgaaggccg aaggctgccg cgcggtggac
     7201 gcccttctgg cggcgcgatc gatccgcaag cacctggaag cgatctgcgc cgccaccttc
     7261 aagccgccgc ttgaggcccc cgcatccgcc gagccgccga acccgccgac gcccaccgag
     7321 tgatccggcc gtccggcccg cacctttgaa ggtcccatga acgccattcc gaaagccggt
     7381 ttcgccgatc ggctcagggc cgcgtcagcc gccaaacagg cgatgctggc caagatgaag
     7441 cccagggccg ccgtcaccga tccgctccac gccgaacggc ccgccatgcg cgcggccgag
     7501 ctggagcggg tcagggccga acgggccgag gccaaggccg ctgtcaaaca ggcgcaggcc
     7561 gacgccgagg aagccgcccg gctggccgag gtcgcccgcg cagaggccga gctcgacgcc
     7621 cgccgcggcc agcgcaagga acgcaaggcc ctgaccaagg ccgaacagaa ggccgcccgc
     7681 gacgccaagt acgccgcccg caaggcccgg caatagcccc tgaccctgac ggagaccgcc
     7741 gccatggccc gcaagcccaa ctacaatttc gaacgcagcg aacgcgaacg cctcaaggcg
     7801 gccaaggcgg ccgagaaggc cgaggccaag cgccgcgccc gcgaatccgg gcagcgcgaa
     7861 gagccggcgc ccgccaccga ggacgacgaa tagagacagc catgcagacc gccaccatct
     7921 tcaacctcga gaccgtgccg ctggacggcg aggttttcgc cgactgcgaa ttccgtcagt
     7981 gccgcctggt ctacagcggc ggcaagccgc cggtgttcga caactgccgc ttcgatgcct
     8041 gcgactggcg cttcgacgac gcggcggccc gcacgctggc ccacctgaag gcggtctggg
     8101 ccgccggcgg caaggcccag gtccaggcgc tgatcaagga gatcaccgtc agcggcggac
     8161 gataggcgcg cgctccggca tcggcttcgg cctagcggcg cagtgcgtcc gatccggcgc
     8221 tccgggtcca ggtccgttcc cggaaccatt tggtcagcac atacttcgcg ccggcttcca
     8281 ccaccgcgcc ctcatggtga gagtagggat tcggcagtcc ctgcccgtcg agattgttcc
     8341 aggtgaccag ggcgccgatc ctgggcgcga tttccacgcc ggccctcgga aaccgggtgt
     8401 ggcctccggt cgcgggcgcg ttgagatata ccatcgccgt ccaggtccgc tgcccaccct
     8461 cctgggcgac cgtctccgaa tagggttggc cggcggcgaa gtagtcgttg tgcatcttga
     8521 attgctgccc gggcatgtag cgctgaccct gcagcgtttc gctgtggctg gccgcgatgc
     8581 cgagcaggtc ggcgatgcgc gtctccacct ccgcgatcag cggatggtcc gcgggcagga
     8641 ggcaggtctc gctggtccgg tgagtcgggc gggcggaggc gctgaggctg gtcgagggcc
     8701 ggacgtcggc gtcgatcatc gcgatcagtc gggcgcagtc ttccggcgac aggaaattcg
     8761 ccgccatata catgtccagg ccggtcgccg gggccctggt tacagacgga acggcgtcaa
     8821 gcctcgcgcg cacctcttcg ccgatcctgg cgagcagacg cggatcggaa cccggaagac
     8881 tggcgtatcg cccggcggga tccggggcgc ctggcgccgc ctggggcagg ggagaggcca
     8941 cgacgcgcgc ggccggccgg atttcctcgc aggacggctc ggcggcggcg cggcgagggt
     9001 caccctcggc cggcccggcg gcggcctgac ccgtggggcc gcgccggccc agtatcctgt
     9061 ccagtaaaga catccggtca gcttagctgt cgcccctgtc cggacaagcc cccgagtggg
     9121 cccggcccgc gaccgggcgg cggaccccgg cctctcgggg ccgatcaggg agatcaccgc
     9181 ccggggcggc cgctcaggac ctcgacctga agccttcgaa gcggaacgcc tcgccctcgc
     9241 gcttgacgtg gccggcggtg ggcgccgcgt aatgcgtccc gatgaccagg atcggctggt
     9301 cagcccactc cgcgaacatc cgcgcccgtg tgacagcgga cgccttcggg tcgctgtcga
     9361 agctcggcga ccagtgggga tgcgccatct ggcagggatg gtggctcatg tcgccggtga
     9421 tgaccgccgt ctgtccctcc gactcgatga ccacgctcac atggcccggc gtatggccgg
     9481 tggtcgggat cagccgcacc tctggcgaca ggcggtggtc catgtcaacg aactcggcca
     9541 ggcccgcgtc gatcaccggc cggacggagt catcgaggat cgtctggttt tcctcgccgg
     9601 cctccgcgga ccagtgctcg aactcgaccc ggcccagcag gtagcgggcg ttgggaaagg
     9661 tcggaaccca ggcgccgtcg accagcatgg tgttccagcc cacatggtcc acatgcaggt
     9721 gggtgcagat caccgcatcg acacgctccc gcgaccaccc ggcctcggcc atcgcctcga
     9781 gaaacgtcgt cgccagcggc tggccgccgg tgatggcgcg cggccggtca ttgccgatgc
     9841 aggtgtcgac caccagccgc aggcccggcg cctcgaccag cagggcgtgg atcgacagca
     9901 gcagctggtc ctgttcgttg acgaagtccg gataaagcca cggggtctgc cgcagcgcct
     9961 cgggcgtcgc ttcggcgatc atcggccgcc tcgggctgta ggggacgggg atttccagct
    10021 ccacgacccg ggtgaccttc accgccccga cctgccatgt cagcatcgcc gcctctcccc
    10081 cgtctctctg cggcaccgag cctaggacgc cccggcccct ccggccagcc acaggctgtg
    10141 gtccgcccgc ttgcgcacca cgatgtcggc ctcgtcccgc gcccggatga tgtgccgccg
    10201 aaggttgggc aggttcatct gcgtccagac cgtggcgcgg gcgaaggcat ccgcctcggc
    10261 ctccgacatg gaccggaagc gggcatagaa ggacgcgggg tcggcctccg ccgcccgcca
    10321 gaaatcgatg aaccgggcgg cgaaccagtc ttccaggtcc gcctcttcgg cgtcgagata
    10381 gaccagcagg tccaggcccg ccgggcgggg cgtcaggccg aggccctcga cgatcaggat
    10441 gtccggcctc gccacctcgc gggccagcga cgggtcgatg tcgtagatca catgcgagta
    10501 gccggggacc gtcaccggcc cctcgcgcgc cgccgccagc accgccgcca ggctcgcggc
    10561 gtcataggtt tccggccagc ccttgcgcat cagcgtcccg cgctcggcga gcacggcgtt
    10621 gggaaacagg aagccgtcgg tcgagaccac ctcgacccgc cgcgcgggtt ccaggatcgc
    10681 cttcaggccc gcgcagaggg tgctcttgcc ggccgccacc gacccggtga cgccgaccac
    10741 cagcaccgcg ccgggcgagg ccctgtcggc gattcgccgc gcgagctcgt cgaggctcat
    10801 cccgctcagg cgtgcgcctt gacgggggtc tcggtgtagg aggccccggc ggtcagcatc
    10861 tcgtcaggaa tggccagcat cttcagatag gcttccttcg gataggccat gtgcggcttg
    10921 ctcgcgtcgt aggccggctg cggcacgggc gaggggccgg tatagggcgc gggagccttg
    10981 aaacgggcag cctctgcggc atcgataccg gccttggcca gcaccgccgg gtcgatccac
    11041 agatccgggt cgatccggtt gccgggcgtg gccacctcca gcgtcatgtg gtcggggccg
    11101 gcgaaataga tcgacttgca catgccgtgg tcgatcggtc cgatgacgtt gatcccgtgg
    11161 ctgcggatgc ggtcgcgcag gccgatcagg tcttcctcgg tgtccgcccg gaaggcgagg
    11221 tgctgcagcg tgccggccgc gctcggcagc gccccgctgc cggcgtgggt cacgccgagc
    11281 tgcgccggga tcttgtcgac gtccggcagc tggacgatcg agaagtagct gtgttcgttc
    11341 atcttcagga aggcgtgcag cccgcccggc acgccatgca tgtcgaacag cgccaccagc
    11401 gggcagccca tgacctcggc gaagaaggcg atgtgcttct tgatgtcccc ggccatgaag
    11461 gccaggtgat ggatgccgga aggttgctgg gacatggggt ttccttgact ctcagccctg
    11521 aattccggac tcagaaacca gaaacaacag cccgccgcaa gcgggcggac gggcgccgca
    11581 ggggaacctc atgagcatta ttcgcgagcc gtacggcttc gcctacgccg aacccagccg
    11641 cctgaaggag acctacggcg ggcccgaggg ccgggtgttc gtcgagtgca tgcgcatccg
    11701 gccggccgag ggcgagtcga agaccgccat cctgttcagc catccgatcg gcggcgggtc
    11761 gttcctgccg ctggtcaccg ccctggccca cgccggccgg cacgtgatct actgcaaccc
    11821 gcgctatcgc gggaacgaca cggccctgat cctcgagaag tgcgtcaccg acctgggcgc
    11881 ctgcatcgcc gacctgaaga agcgcttcgg ctacgaaaag gtcgtgctgg gcggatggtc
    11941 gggcggcggc tcgctgtcgc tgttctacca ggagcaggcg gtccatccga cgatcaccga
    12001 gaccccggcc ggcgatccgg tcgacctgac ggcggccggg ctgcagccgg ccgacgggat
    12061 catgctgctg gccgcccata tcagccggtc ggtgaccatg accgaatgga tggacccgtc
    12121 gatcctcgac gaggacaagc ctttcgagcg cgacgtcgag ctcaacatct acgacccggc
    12181 ctgccccaac cagccgcctt acagccccga attcgtcgcc cgcttccgcg aggcccagat
    12241 cgcccgcaac cggcgcatca ccgcccgggt caaggcgacc ctggccgagc tcaagaagac
    12301 cgacccggac atggagcggc cgttcgtcgt ctatggcacc atgagcgacg tgcgctggac
    12361 cgacccgacc caggatccct ccgaccgcaa gccccatacc tgctacctcg gcgacccccg
    12421 gatcgtgaac gacgggccgg tgggactggg ccggttcacc acccttcgct cctggctgtc
    12481 gcagtggagc tacgacgaaa gccgcgccca tggcctgaag aacgccgcca atgtgacctg
    12541 cccgaccctg gtgatcagca acaccgccga cctcgcctgc acccccagcc acgccgcgcg
    12601 gctgttcgag gccctggcgt cgccggacaa gaccttcgtc gaggtgaagg gcgccgacca
    12661 ctactacatc gagcgctatg acctgctgcc ggaagcggtg gccgcggtga cgggatggct
    12721 cgacgcgcgg gggttctagg gggcgaaagc ccccggagcc cggcgtggca gggaagaaat
    12781 ggcgcgcccg gaacgaggtg tcgccaagta gcaaaatatc tcgctaaaac atatacatcg
    12841 cagtggggaa cctgcgtctt ggcaccccga ttttgcaccc cccgctaacg tcgcctgggt
    12901 cgcggcggct tcttgacgct aacgacccac tctctccctg tgaccggtga gtgcagaaaa
    12961 acgtcggaca tcaaatctac gaagtcctga acttgacgac gatctctcgc gtcgatgcgc
    13021 aagcggaagc cttcgccatc cgcctcgcaa acgatatcta actctttggc agcgatgcgc
    13081 tcagggagcg cccctacgtc aatgtcagca acgaggccgt gccgaagcgc cgatgcaagc
    13141 ttcttggcca gcagcgggcg cgctccgatt gcctctttga gcccctcggc gtcgtcgagt
    13201 gcaaaccgct cacccaaggc gtccaccgcc tcacccgcct tctgggcggt caccctgcgg
    13261 atgttcgtaa cggactcgaa cgtccgcaga ttgcggacga agaccagtcc ttcccagaga
    13321 aagaagtcca cgtcctggtc gaaattcact acggttccac ccacggggac aagctcatcc
    13381 gcatctggcc ggaactgcgc gatcacggca ttccgccgcc gcagggcgac gttcaagttg
    13441 cggcttcctc gaaagttaag aaggacgcgg ccatctggaa acgaaagacg cgtgacgtag
    13501 agcctcgtgt tctccaggac atcttcgtcc ccctggaacc gttgcggcca atccgggact
    13561 ggcacttcct caagccattg cccgagaccg ggagtatcgg cgacgctcag cacgccgatg
    13621 tcatggtccg ccatcgcatc gaacgtgaac tcttcgatca agtcctcgtc cgccgccctt
    13681 gctgcgattt ctctgagtgt tgtctcggcg gtctctacca tctcatcagc aaggttcccg
    13741 gccatgctga gcatgtgcca agggtcacgc ctccggcggg tcggcttcga tttcaagaag
    13801 acatgacagc ccacctgctc cgcctcatca aaggcggaga ttaggtctgc gagtgcagtg
    13861 tagccgtcgt caacgagcgc cattcgtcac ctgccctact cggcaaagta tagacgagtc
    13921 gcatccacct cgtagagggc gagccggtca cctgtgatcg tgctcgtgcg ccgggtgacg
    13981 atgacgactg agcggtcgct ctccaagcgg acttgatgca cccgatagcc acaccacagg
    14041 aagaaggggt tcacgtagat gctgtcggct ttgagcatca ggaggacgat gatcgcgtag
    14101 aaggcgaata ccggcacgat cgtttccacg gtcccgaagt cgtccatcag cacgggtggg
    14161 aggtaggaaa tgaggtgcga gagaacgtgc tgatccaagc gcgcatcgac gcggactacc
    14221 tcgaagctgg gggacttccg cttcatgccg gtggcgagcg caaggaaggt aacaaacgcg
    14281 cagagagcga gcagacctgc gactgccgcg ctccagggcc ggttctggac gacgaacacg
    14341 acggccatga gggcgaacaa ggggccgaag gagctaaaga cgaactggag acggaagaag
    14401 ttcgtcatgc cccaccgtcg tgaacaaaca aagaacgata ggccgcgcgt ggccgatgag
    14461 caaggcttcg tgcctacttg ctcccgttgg ccgcgcctcc ccctttatgg agcaccttag
    14521 aggcatcaaa gaccagcatg aacttcgctg cgtcagcgtc gcgctcgaac cgatagtgaa
    14581 gccgcaacat gtcgcccgga tcgccacggt tccagcgacc ccggcagttg tcggcgcacc
    14641 actcgtctcg ctccgccctc atctcgacaa aggactccaa gtcgtcggcg gggtcacaag
    14701 cgatgacggt ttcgtctggc catcgcgatg acccgaattg ctccattaga cgcctcttcc
    14761 aatcctgcat gtcgcccctg ttaatatatc atagtaaaag tgcttacgga gtgctcgaaa
    14821 ggcataggct ctacctcctc tgaaatgacg actggccgat ctactatgtc aaaccgggat
    14881 ggatttattg aaagctttac gctctgaaag ccccttgacc atgacccgag ccagttgtga
    14941 tattccgcga cccactcgat tggccaagtc atggttcgat cggcaagttc tgtcttgtgc
    15001 ctatgcttca atgcgtaaga ggcgacctgc ttgatattcg cttccttcgt cctgttccga
    15061 tgtagccgga cgagatggag ttggcgggtt ccgtgccagt gatccttcaa ggcccgctcg
    15121 acacgatcaa ggcccacttc tccgctgtcg atgaggccgt gacaggtcac ccaccacacc
    15181 gggtcgttta gacggtagga gagcggcccg atctccgcga actgcgccgt cttgatcggt
    15241 ttgagccggg ggacatctgc tcccaaaacg gcatcgactt ctaaccacgc tatcgcctga
    15301 acggtgcgcc acgcttccga ccgccgccgc tggaactcca tgacgttgcg cagagctttc
    15361 ccgaacccct tccaggttgg accgatggca tcggtccccg gcacagcgcc gatgacaacc
    15421 gtgagcatgg ccatgtcctc gggagagcta tcggcgaact ggcgtagcgc cgcccgcttc
    15481 tgcgcctgga cgtaacacca gcggcatcgc tggcaggcca tacgctggca ctggaagcga
    15541 ccgtgccagt cttcgccaca ccggtccacg cgacggacca gggcgagcga cgtgtcggtg
    15601 ttcatgcgga caagttcgga gcggagcggg tccaccagcc agcgggggcg tgacatgacc
    15661 gtatttagct tcggggccac aaacgcccct ggcggcgcgt cgcacggccc tggcgagcgg
    15721 cgcatcacgc cgaaatggac gttcggcgag cgacggtggc atcgtcatgg gatgactaac
    15781 cgccacgtcc ccgtcgtcac ggacggctcg ccatcagcct tcctcgacag gctcccacat
    15841 ctcgcggggc tgtcggtttt ccacctgccg agcctacgca cggtcgaaga cctcgttacg
    15901 tggcaagctg agggaccgga gctaatgggc gaggtcgcag agtggtgcca ggacaccaac
    15961 tggacgggtc aggtctggat caaccgatcc gacgcacccg cgatgatgag cgtcgcgatc
    16021 gaagcttccg tagacgcggt gatgttcgcg cttagatggt cggacctgat cgggagtcag
    16081 caccgctacg cggcgtgagc caacgccttg gcctgcggga gcgcccggta gagggtcgag
    16141 acgccgaccc cgatctccct tgccacctcg gccaccggca gcttgccctc gatgagcagg
    16201 gccttcgcca tcgcaatctt cggctcggtc atcactttgg ggcgaccgcc gcgttggcct
    16261 gggccacggg ccgcaagagc cgccttggtt ctggcgcgca aacagtccac ctcgaactgg
    16321 ttgatcgcag ccatgagatt gagcagcagg cttccagacg gacctcccgc cgtggtgtcg
    16381 atagcctcag tcaggctcct gaggccgatc ccccgacgct ggagatcatc ggtcgtggcg
    16441 aggagttgct tcatgttccg ggcgagccgg gacagggcgt agacaacgat ttcgtcgcct
    16501 tcgcgcgcga acgccagggc cttgaggaac tcgggccggt cgtcgatccg accggacatc
    16561 ttctcttcgt agatgtgatc gcagcccgcc gccttgagag cgtcgatctg catcttggtg
    16621 tcttgtgaag agaggctgac tctcgcgtat ccgatccgca tacacttctc gtaactcaaa
    16681 accccaatct gtaaagagag atcagttgcg ggaatgagtt atgggagacc ttaacctaag
    16741 atcgcactac attacgggaa gcagcctgct cttctcaaaa ccgatcggtt tcgggaaagg
    16801 caggtgagag cggggcggcg ggggtcttca atagaacggc aaacggggga ggatgccctc
    16861 tgcccgttcc agcgcctcgc gctgctgacg cggcgaaggg cgacgcaccc ggacttcatc
    16921 cagtccgttc agccgagcca agacgtgaaa tgcccacttg ttgaactcgc tcattgctgc
    16981 cgagccttcc tggtcatcgg catctggcag ttccacctgg aagagttgac cggtcgcgcg
    17041 atcgagcacg acatcccacc agacggctgc tcccggaaga agaaacagcc acgtcgcgcc
    17101 aatgtaggac caatcgttcc attcagcggg ctgcaagaca atgttgcagc cagcgaaccg
    17161 gatcaccgcc caatcctcca tttcggcatg aaaccagcgg tccctgatcc ggctcatctc
    17221 tttgcgcacc attgccgacc cggcgatggt gcggatcaca gtcacgacgc tgcgttcccc
    17281 aacagccacc attccgttct ccctcccgaa gcgcgaaggc tctaatccca agcgtcgaac
    17341 gcgaccaagg cgattccatc ccagccgcca aacgcgtcgg cgaccccggc cagttcgtcg
    17401 ccgggttcga cgcaaaccgg gcagaccacc cccaatggcg cgagcctgtc gcggacccgc
    17461 aggagggtct ggcgctccac ccgctggttt cctgccgcgt gcacgcgatg gacagcccat
    17521 gcgccactgt cgaggagccg agtctcgatc aacagttccg caccgcgcca aaggatcatc
    17581 gcccaaacac cagacgcggc ctgcgggagg atgcgctcag gactcaagca gttgcggaag
    17641 ctctggccga cctcccgcgc cttggcgatg ggaaacctct ccaggtccgg gtccgcgtcg
    17701 caggggtgcg tttcggggaa caggcggtcc gccctcgaca gcatcgcggc caggaacggt
    17761 cggctccgac gctcccgcgc ctgggtgacg gcctccttga tcgcatcgtc agtcgcccag
    17821 gagcacaggc ggcggatgat cgcgacatca cgctcgaagc gccggaccgc ctcctcgccg
    17881 tcgagaccat tcacgacgcc aggaacgagc aggccgggat cggacatcgc gaggaccgcc
    17941 tccaggcgac actcgtccag ggtgccgcac tgttcgaggg ctgcgcgacg acgccgatcc
    18001 aggggatcgt cagaggcgaa cagccggtgc agcagtccat agaaatccgg caggccgaac
    18061 ggttggccat gcaacttcct gagggccccc aggagcccat ctgggcagga gcgataactc
    18121 gcttcaatca agtcgcgcgc cgacgccttc ggggcgaccg cagcgaactc ggcaatgaca
    18181 ggccgacggc tcgcggtaag ccagccatcg tcgccggtga ggcgaaagac cacttcgccg
    18241 ctcgtggccc cggcagcagc gaggcatgcc caaagcgcct gccgccactg caaccgcgcg
    18301 cggcagacgc gcgccaaaaa gcccggcgca gcgtcgtcga tttgcacgac ctgatcgagc
    18361 gcccagcctg agtggactgg gaaaagcgcc ctaccgccgg gcaaggtctc cgtgccgaga
    18421 ggattgtcgt cgtccatgtc cgcctccgat gaggcggctg ataagttcag ccgcgtaaac
    18481 agcggcgggc ggggacgact tggatcaaca cgtcgggcat tgtcgcatgg atcagcgatc
    18541 cgtccagtgc cgctacacgc tggcggcgca gatgcaatca gccccggtta cgagcgatct
    18601 gctctccgac agcccgtata gtttcaaaca ggccgtccgt gggcaccgtg acacaacgcc
    18661 cccccaaccg ggactggaag ggctctgagg tgcgcagaaa aatcgcgatc tcgccagacc
    18721 gtgggtccgc ctcagaaagc ttttgaagga aatcggtaaa gacgaaccaa ccagtcaact
    18781 gcacaccgtg ctgacccaac agcgaaagct ccccctctac caaagccaca ctgctgctac
    18841 cgacggcaat ctctactttg ccgactgaat caatcgcctc ctggcgatca gtgttcaact
    18901 cgtccggcgg cggaattagc gcggcaaaca ggttgattcg atggcccgct gctgggatca
    18961 ggctgacgcg cagtcggaaa cccgacggtt gttcgtggta gcgtgtgaac gccgtcatgc
    19021 ctccaaactc accgacatcc caacccgcga actgtcgctc cagtgcggcg cgggcatcgg
    19081 aagaggatga tggcttacgg gccgggacac ttggacgagg ctcggctttc gtgtgccgcc
    19141 gatccgctgc gccctcaacg acgccacggc gcgtttcctc ggtttcggcg ctcaagctct
    19201 caggggtcca gtagggcttc ttagcccggc tcgcgatatc gcgaagggga tgaaagtggc
    19261 ggcagatgcc caagccaatc ttcgccttcc gcgcccacgg caccgcctgt aaggcttcta
    19321 cggacttctt gatccagccc gatacgattg gcaggccgca ctttgggcat ggtggcgcgc
    19381 attcggtgca gaccggaagg ctccaccgtc gcccgatcga gttcttcccg atgagcaggg
    19441 ctggggttct tggcgggcac gaagcatctt catagaaatc gcagacgtgc agtgccaacg
    19501 gacgatgctc ctgagcacac agggcgcact gtgcccctgg cagatgttgc gccttgtagg
    19561 atttcagaag ttcggcgcgc agcctcaggc gttcgtcttc ggggatcatc cgaccgagga
    19621 ctgctcgtat gtctttcggc ggcatggctc ctccaaggca ctggccctgc accttgatgg
    19681 ttcgctccgg ttagcgtcaa ccggcgcagc aatcgttggc tgctcaatgc tctacgctat
    19741 cagccgcccc gtgccaggat catagcgcag cccgacgatc gagccgtcgc ggatgtgctc
    19801 gacggcctcg tcgatggcgg cgatcggaag caggaaccac tccctcggtt tgacaggatt
    19861 gccgaaccgg tcggtcaggg tcaggtcgag caaggctggg gcgaagacct tgtgcaacaa
    19921 accctcaagc tggcggcagt tcacgccgaa caccttgtag gtcgcgacga cctcaacatc
    19981 ggccaagagg taggtggcgc tcttgtcggc ccctgcgagc cgcgcctcga cggttccgcc
    20041 ggtgacgccg atcttgtgga tcacgtcgcg gtgctcggcc acgaacgggt ggtcggactt
    20101 gctgcgcagc acgtagatcg tgcccgtttc gacatccccg tcttccgctt cgctggcgaa
    20161 gatcggggca gggccgtccg atacgcccgc gaaaagcggc ccagcctcgg gctcagtgat
    20221 acggcggccc gcctcatcct tgtagagggc gcgctgcaat gaccgcagga gcaggtcgct
    20281 ctctgtgccg ttcgaataga tcacccgcag gcgcgcatcg gtttcgccgt tgggggcctt
    20341 gatcggctcg ccgacggcgg cgacgtaggc gatctggccg cccaggatga agaactcccc
    20401 cgcgcggatg tccgccttct gaaagccagc gtcgcggacg aacgggcgcg tcgagcgccg
    20461 tccggcatcg aggtcctgct gcacctgctt gaacagcggt tcgaacttgt cgaagtcctc
    20521 gcaccgttcc cggttcgcaa tctcctcggc agcccgtcgt tcttcgcgag aggggacgtg
    20581 gcgaagctgg gtgatgtcct gcgcgtccga cccggcgagt tctgcgcgaa gcgcctccag
    20641 gtccatctcc tccggctcga cggtcttcgg ggaggcatca agcaggcggt gcttgtcgag
    20701 cggagcgagc aacgaccggc attcctcctg gtcgcggaga cggtcgagcc ggaccgcgta
    20761 gagacgctcg aagatgtccc ggtcctcccc gtgcagcggc gcacgaccgt gctcctcata
    20821 gaagcgcaca atatcctcga acccggcgat gatgcgttcc tggcgtggcg tataggtcgc
    20881 cgccttcgcc tcggcggggg cgaagtcagc cagttcggca cggaggtcgt cgaggctcag
    20941 atcagccata tcggccttct cggtcgtaac gcatgaaggc ggcagcgccc tcggccagct
    21001 tcttctccca ttcgtcggca gcgtcgatcg acggcgggcg gccccgttcg cggcggaacc
    21061 gcacggcgcg ggcggcgtag tccttcgcct cttccggcga gagggtcgtg cgcttggccg
    21121 agatgaccga ctggacctgt ttgagggtcg cctcgttcat cgacttggcg aggacggcgt
    21181 aggcggcctc gaacgggttg atgcggtcga tcaggtctat gtccaggtcc cggacatcca
    21241 tcgcgaactt gcgcacgccg tcgagcagcg ccgtgttggc cttcggctcg cccgtggcgc
    21301 tgtcgccgcc ggatgccagt tccttggcct tctgcgtcag gttcagcgcg gcgatggcgt
    21361 gctggcggat cgcttcctga tcctcgggac tcatgtcggg gtatttgtcc ctgacgatct
    21421 tgcccatgcg gacctgggtg agttcctcgg gaacagtctc ctcgtcgaac aggccacgct
    21481 ccagagccgt cttgtcctgg acgaatgccg tgatgacctc gttgaggtcc tcccggcaca
    21541 cgcgtgccgc ttccgggctc ttcggctcga ccagacccgc gatctcgaag tgaagttgtc
    21601 cagtcgtctc gttgaccccg acgttggtct ggcccggctt gtagccctcc tcgccgtagt
    21661 cgtagccctc gaccggcccg tggtccttgg gcgtgaagtt gaagcggggc gcgagcacct
    21721 gctccatcag caggctggcg gcgatggcct tgagcgtgtc gttgatggca tcgacaacag
    21781 cggcttccga cgcatcgggc tccgcgatca ggttagtgaa gctcgcgctc tccttgcccg
    21841 gcgcatcccg cgtcgcgcgc ccgatgatct ggatgatctc ggtcaggctg gaccgatagc
    21901 cgaccgtcag cgcgtgctcg caccaaatcc agtcgaaccc ttccttggcc atgccgagcg
    21961 cgatgatgac atcgacatga tcgcggttgt tccgctgagc agggtccttg agggccgtca
    22021 gcaccttggc ccgttttgcc tgatcgctgt catcgaccag atcggcgatc ttaatgatcc
    22081 tgccgcctcc cttgagacgg acgcgatgga agcccgtaac cgggtcggcc tcgatccatt
    22141 cgccgagaga ctggatgatg tgctcgactt ccttgatctt gtccgccgtg ctctcgcggg
    22201 agttcaccga ggggatgtgg acaatcgtct tcctggccgg gtcgagcacg gcttcgatcg
    22261 ctgagagata gcggcccgtg tagaagaagt agccaatatt cagtgtcttg aggtgctcat
    22321 acccgttgag ttgttcgtag taggtgtagg tgaccgtctc gaacttgttc tcgtcctccg
    22381 gcatcagcac cggggccgca tcgccccgga agtagctgcc agtcatggcc acgatgtgag
    22441 ccttgtcccg cttgatgaac tcgcccagtt ggctgcccag gcggttgtcg gggttggccg
    22501 acacgtggtg gaactcgtcc acggcgatca gccggtcatc gaacgcctcc acgccaagct
    22561 gatcgacggc aaaacggaag gtggcatggg tgcagaccag caccttgtcg tcgctggcga
    22621 ggaactggcc gacggccttt accttcgacg aatcgacccg ctcctcgtcc ggccccggtg
    22681 cgttgcacag gttccactgc ggcttcaccg tccagtccgc ctcgaagccg aacttcgaca
    22741 gcggctcgtc ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca gtgcgcgccg
    22981 tggtgaagct gacagcgggg atcggtttgg ctgtggtcat gctcaggcgg cagccttctt
    23041 cggtccaccg ggcttggtgg tcatcttggt gtagagttcg aacagctttt ccagccgctc
    23101 ggtgtcgttg cggaagcgcc gaccgatgta gatgcgttcg agcacctcgt cattggcctc
    23161 atgggcgtga cggaggtctt ctggcatttt ctcgggatcg tagaggtccg cgattgacgc
    23221 ggggaagtga gcttcacggg caagcaggat ttcttctgcg cgacggatta agtcagactt
    23281 gttttgagat gtgagatttg gaacgggaaa ggtattccaa cctatggtgt ttgaataaga
    23341 aaacctcatc tcaagtctaa cgcagaccgc gccaatccaa acccaatgca acctggacgc
    23401 cagcaatgcc aaggaccaca agggtccgtc gtatataccg tagttcttgt tgttgattgt
    23461 ggcattgcta tctatgagcc cgcaaggcaa atactcacga ttctcggatg agatggcagc
    23521 agcgaccact gtgtgacgct ggccaatcat catctctctc atctggtggc tccgtgccgc
    23581 catagtgttt gccgctggat cagggctttt aagccgcatc gcctttaccg ccgcgaaacg
    23641 ctcagcgagc gcgggcactg cggctgcttc ggcaaggtcc tgatcggaaa tccaaagaca
    23701 gtatcgctca ataccgcgaa taaactcagc gccgccgtag ttcttgcgaa gaaatttccg
    23761 gcgttgcgta tcagttagac caagagattg ggcctcggaa accgtgagca gtagatggcc
    23821 gccatcaacc ggcttgttcc caaagctcat acgcggcagg tcgttcagcg ggcgtgatga
    23881 cttttctaca aaaacatctg gccctggcac aaggtaggcg ttgatgttct cgacccgtcg
    23941 gagagtggtg tctccgttag ggacatcgtc gtatagtagg cgggacgaac cacactctcg
    24001 gccaatgccg acgattacta ccgtcacccc agctttattg ctggcgaggt ttgcccattt
    24061 gaacgaggaa tgagcaaagc agattgagtg gccaaggttg aatatcgcgg gccacagcaa
    24121 aggcacttgc tggccttggc aaatcgagtt cgtcgctacg aatgcagcag cggaaggcgt
    24181 gtgtgttcca tactctgcgg ccttgatgaa ccaagcggca acgtagtcca gggttttcca
    24241 gctacgaacg tggcgcgcaa cggcccgctc aagatcggcc ttctgttccg ctgactgcca
    24301 cgttgaccct aaatagggtg ggttaccgca gatataggtc tcgccccctt cgttctcgaa
    24361 gtcgatctca gcctgatcga ggggcgtttg gaataggtcg tccgaagtga ccttcacccc
    24421 cgtcccggtc ggcggacaga tgttcagcca gtcgagacgc agcgcattgc cgcaggttat
    24481 ccagttttcc gatccgagcg gcaggaagtc agcgaccgcc tccttttggc cgcgacagag
    24541 cacgtcacac tgatactctg cgatgatgag cgcgagcctc gcgatctcag ccgggaagtc
    24601 ccgaagctcg atgccccgga agttggtcag cggaatatcg gatcgacggt ctggctcact
    24661 ccgacgcttg ttgatctcgg cctcgatccc ccgcatctct ttgtatgcga tgaccaggaa
    24721 gttgcccgac ccgcaggctg ggtcgaacac tctgatccga gatatccggt tgcgcaggtt
    24781 cagcagcttg cgcccgttcg tgcccgcctc ctcaagctgc tgccgcaggt catccaggaa
    24841 cagcgggtta agcactttca ggatgttcgg cacgctggtg tagtgcatgc cgagcgatcc
    24901 gcgctcctcg tcgtcggtga ccgcctggat catcgagccg aagatgtccg ggttgatctt
    24961 cttccagttc aggctgccga tgtgcagcag gtaggagcgg gcgaccttgc tgaacgtggg
    25021 cacatcggtg ctgccgctga atagctgccc gttcacgtag gggaatttgg tggcgtaacg
    25081 cggcagactc gccgcctcac gatcctcagg cttggtgtcc atcgcgcgga aaatctcgcc
    25141 gatgacccgg tgcgtgctgg cggcatcgcc cgccgacatc tgctcgaccg tcttcgtgaa
    25201 cagcccttct tcgaggaaaa tgtcggtgtc ctcggcgaag aagcagaaga tcagccgcgc
    25261 catgaaatgg ttcatgtcgt gctggcggtc cgctttggcc cagtcggggt tctccttgag
    25321 cagttggaca tagagccggt tcaggcggcc cgtcgccttc acatcaaagg agctttcgcg
    25381 gatttcgcgg accgtggtga tcccggccag gggcaggaag aagccgaaat ggtcaggaaa
    25441 ctcgacgtag ctgcaagcga ccgtgccgcc gccgttcagg tcctcggcct ggaacgcctc
    25501 gccatcggtc gccaggatga acttcgcctt ggccgaagcg gtcttcggac tctcccgcag
    25561 ggccttgagc gtcgagtcta cttcgccggg tgcgcacgtc acgatgtgaa tgttgttccg
    25621 ctggagcacg cccccaggga tgtcggactg gttggtggag cctgaccgaa ggcgcttgat
    25681 tgtcgtctcc gggttgtcga acgcgcggag gaactggagc gggaactcgg cccgatcgaa
    25741 gggctcaagt gcaaggttcg aaacggcttc ttcgatctcg acgggattca tgcggccagc
    25801 ttaggcggag ccggtccggt cgccaacggc gagcacgcgc gctaccactg ttctcgatgc
    25861 tgagcttcaa gccattgctg caatccgccg ggttcgaccc caaggaggtg aaccttctcc
    25921 gccactgtcc aggcgggatc accgacccgt tcggtgcgtg gcgcgccgac cgcgcggcgt
    25981 tcgaggccta tcagtcgatg caggcacgtc gggatcgcag ctatttcagc cgtccttact
    26041 gggcttcgtt cgcaccgacg cgacagggcc gaaccatctt cctcggcatc tacgaggcgc
    26101 gcttaatcgg ccccgtcatc gagggcgaga tcgacgcact gactggcgaa gtgctccggg
    26161 aagaacgcgc agaccgctac gactgcaagc tccgacccga actgtcggac tacatcggtc
    26221 gcctcgtcat cgactggggc gctggcactc gctcctgggg gcagaagggc acgaccgaca
    26281 aaccgatcat cgaactcctg cgaggtgtcg aggagccgcg cttccccggt tatctcgaac
    26341 tgatcgagcc cctttcagca gttccaacct tcccggaacg ctgggtcgat ttcctacgcg
    26401 aagggcgtgg cgtttatctg ctcacctgcc cccgcaccaa ggagcaatat gtcgggaagg
    26461 ccgacagcgc tgacggcttt tgggggcgct ggatggccta cgccacgacc ggcgacggcg
    26521 gcaacgtccg cctccgcagc cgggaacgca gcgactatca ggtgtcgatc ctggaggtcg
    26581 ccgggtccgc cgccagcccg gacgacatcc tgcgcatgga agtccgctgg aaagccaagc
    26641 tgcaaacgcg cgagatgggg ctgaacggca actgaccccg gtcagcggag gagcatcacc
    26701 agtgacagcg atcattctgg ctcccacgcc tgaggtccgt gcggcttgtg accggattga
    26761 tgacgccatc agtcgggcgc tggagtcgct tggccgcacg atcaccgccc accaaacatg
    26821 ggggacatac gagtcgagcc gagatgcagt cgcgctgttc aaagcttctg ttcggtctgt
    26881 cgagtccacc attgctctcg cccgcaccga tctcgttctc tttccgggcg cgctgatgac
    26941 agcgcgaggt gcattcgaga tcagccttcg cgccgcatgg ctcgtggatt gcgatgaccc
    27001 tttcgatcgg gagcgtcgct ggcttgcgca cctccacaac gaggagaaca gcaacggtaa
    27061 ggtcgcggcg catctaaaag gggccggagg cgatcccgag tcgttcctgg cgatgcagga
    27121 gcaaatgagg acctttaggg cagcgatcga ggcgaagttt ccgcccggct acgcggttgt
    27181 gccgaaggtg ccgaacgtcc cggagatggt gcgcacgctc cacgaggagc ctgtctacat
    27241 tctctactgc tacctctcgc agttcatcca cggcgcgagg cgagccgcga actattacgc
    27301 gaccgatggc agtccccgaa gagacgcggt gacccccaag gattggtatc tccctctcgc
    27361 ggtgtgctgg atcagcctat tcagaggagc gagaaagctt cttgagcgtc ttgacgggcg
    27421 accggatgat ttcatgacga tagaggaatc gaccgagatc gaaggcgcta tcgctgcggt
    27481 cggggccacg gcttgagatt cagcgttcgt cggatggcag acgggcaacc aactcgacct
    27541 ctcagcggac gacgctgaaa tcgactcccc gaaactcgat cttctccata gcctttgcga
    27601 gtgcagtgag attgtagccg ctgtccttcc gcgcgccatc gcgggccttg ccgtatccgc
    27661 ccccgacacc tgagacgtgt ccgcacaatg cgtcgattac ggctgcatcg agggtatcgc
    27721 gggcgacccg cttgaaggtg tgccgaaagc tgtggaagcg tttgcgcggg tgatcgaccc
    27781 ctgcttctcg gagataccgg tggaaccatt cggtccagcg ggtcacacgg ctatcagtat
    27841 cgagcccatc ccacaggagc ccggtcggcc cgactgcctc acgtcggcga tcaaggtagt
    27901 cgaggaagcc cagttcgacg gccttcgggt gaaggggagt ctttcggatc gagccgagat
    27961 tcttgacccg ccgcccgctc tcaagctcag gcgcgatctg aaggtagcgg atgccggttt
    28021 cgtcgtcggc gacgacatcc ctgacccgca aaggcacgag ttcgccacgc cgagcgccgt
    28081 ggagcatcgc cagcagcggc aaccacttcg ctgcctcctt gcgaccgcgc tggggtcgct
    28141 cgcccctgct gaaaactgga gatgacagca acctgttgag gtccttcgcc gagaagggtt
    28201 cgtagctgcc cgtcgtctgc gggtcgatca caagctgaac gccaaacggg ttcttccagg
    28261 ccgggaatgc gtccagatcg ccttcgcgct ccgcgtgaga tgcgatcgcc gccagaagat
    28321 taagcgactt gttgatcgtc gtggctgtgc gcgcgggata ggcggagagg tcttcctcca
    28381 gcagtcgcgg cagaggtgta ttcgccagcc gcatcggaag cctcgcgggc acctgggcaa
    28441 acttgtccct gaactcgcgg gcctgacgtc ggcttatctc ggacaggggc agatcgccgt
    28501 gcaactcaac gaaacggcgg acggcgagct tcgcgtcgat taccgtccga tgttgacgtg
    28561 tccgtgctga gcgggccgcc gacatgccgc tccacgccgc gaacgcctgc tggagggtca
    28621 gtgaggtggg tctcccaagt gggggttccg ccgcctgccg ggtcggcgga ggggctgtag
    28681 gcaggtctgc aaagtcgttg ttcagccagc ggcgttcctt ggccaagatc gccttccaag
    28741 cggtaaggac ggccatgtag agggtttgat ggagagggtt tgggaccgga ttctcttccg
    28801 gtggcaggct ccaaccctcg gcccgcacga tttccgcgat ccaggcgcga acttggcgct
    28861 cctcagcagc catgcgctcc atgaactcgt ccggtgactc tgtttgccca gatcgtccaa
    28921 acgcctgtgt aatcctcgat tcgcgcccga acgcggccat gcggcttcca gggccgcgcg
    28981 cagtgatctc acttgcacgc cgatagagtt cggcttgttc ccaacgctgg acagcggcag
    29041 cgatctcgtg gaccggcggc gggtcagacg catccgacaa agggggtggc gaacctttag
    29101 ccgatcgggc ggcctggaat gcgcgctcta cgcgcgccgc gacctcgtgg acgcgtagcc
    29161 gagcatccgc gacatccttg gtgttgaggt tttccctcca ggtcgtgccc cggccaatga
    29221 catctcggat gtcctcagga attccgcgct ggaacttgaa gactcctgac ttcgatcggg
    29281 tgaggtagcg aggtagcgcg ggcactttgc atcccgtttt tgcaccccgc cagcatgcgc
    29341 aaacccttgt gggagcgcaa gttcaacggt gcaaaggaac gggaagaaga atggcgcgcc
    29401 cggaacgatt cgaacgtccg accctcagat tcgtagtctg atgctctatc cagctgagct
    29461 acgggcgcgc acttcgcgga ggccgttcta ggcctgctgc gagggcgcgg aacctagtcg
    29521 ggctctggcc gaatggcaag cgcccgaccg ggcctttttc aaacaatcag cggccgcccg
    29581 gaatcgccgc ccccagaccg cgcaggatat cggtggcgcg cggccgggcg ggcttggccg
    29641 gtttgggttt gccgggcgtg gcggcctggt cgccgccata ggcgccgggc atctccatgc
    29701 cgagcatgcc ccccgtcgcc gagcggtagc ggaccttgac cgtccaggcg atgttccagg
    29761 cctgcctggg gtcggccggg cgttccgggt agctgaagtt ggcctcatcg ccataggcga
    29821 cgaactggta catcgactgc ggcgcggccg cgacgacctc cttcggcacg gcgcagctgg
    29881 tctggctcgg ggccatcagg gccttgctgg ccagcagccg ggtcacgtcc gacggcttga
    29941 ggtagtcggg cagggcgaac agcaggccgg agacctcgct ggagctccag accaccacgg
    30001 tctcgccgtc gccccccatc gtcgtggcca tgtagccgac ggccccgtcg acgggccgcc
    30061 agcccagctg gacgccgccc gtggcggccg ggctgttggt cgtcatctgc accggcggca
    30121 ggaagtcctg gtccggcgtc atcgagaact ggatctgcgg gctgtagttg ccacggatgg
    30181 tgtgggtccc ggtcagggag ccgtcgggcg gcacctgggt gcgggcgcgg tcattgggcc
    30241 attcgccata ggtcctgctg cgggtcggcg acggcggctg catggcgctg acctgcaggc
    30301 cctgcatcat ctgggtgaag gccggcggga cctggccgtc ggcgactttc gcgaagtcga
    30361 tgaccaccgg ctggccgggc cgggcgcgct cgccgcagcc ccagtagatc agcatccggc
    30421 cgcgcggctt ctcgtaggac tgcggcatcc ccggctcctc gcgcgtggcg ggggcggtga
    30481 cctggcgcgg cgtcagcagc ggcaggctgg gcccggcccc ga
//
LOCUS       DAORNX010000002        27764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_1310_length_27764_cov_8.669746, whole genome shotgun sequence.
ACCESSION   DAORNX010000002 DAORNX010000000
VERSION     DAORNX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 27764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27764
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_27764_cov_8.669746"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLO65_00160"
     CDS             complement(<1..244)
                     /locus_tag="PLO65_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRD26690.1"
                     /translation="MSRRVYLAACQHETNSFSPIPTGRASFEDSLWLRPSESPGAGDS
                     GLASVVGYGELLARARAAGWSTVLGPAAFAQPSAPAT"
     gene            complement(244..1695)
                     /locus_tag="PLO65_00165"
     CDS             complement(244..1695)
                     /locus_tag="PLO65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD26691.1"
                     /translation="MSAFPSVSVFSGDLDARINAILAAYDAPGAAVALVSKGQAFASG
                     FGRRHLALPDPVGTTTAFNVGSTSKAFSATAVAVLVARGIMNWDDPVRRWLPDFTTWR
                     ESDAAAMTLRDLSGNRAGLPRNGILEFGTELTITAEEVVRRLRFSPPAAGLRERFTYS
                     NIAHTAVALAVERASGLAYVDFLKREIFGPLGMKDASGGAVAQTDLKDPAGWHCALDG
                     ETVEIPPIFTDVHRGSAGLCLSAEDAARWLAFNLGDGSPILPASGLAELFEPQIALRE
                     EELAIWIAPPGATGPAYALGWGVAWQNGVRVIRHSGSDFGINAHVALAPAERSGVAVY
                     VNKDCKASIEISYAVLDALMGWSPGDWRDVIFDRKLPDTNAGFKHSTLRPNGARPDRP
                     WDTYAGRFHSRREGALDVAVQAERLSLRFLDAPVFDGELAPLGGDRFLAVPDYSGLVS
                     DAVGGRFEAEFDLAGDQVRSLTIHGIGVFERAG"
     gene            complement(1699..3054)
                     /locus_tag="PLO65_00170"
     CDS             complement(1699..3054)
                     /locus_tag="PLO65_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26692.1"
                     /translation="MTSRVPIGQLAAFVLPKLALGLITAPFFYVLPTFYAQNTKATLA
                     GLGTALIASRLFDAFTDPLVGWLSDKTKSPWGARKPWIVGGAILIAASIFFLFQPGGD
                     ATVTYFLLWSLIFYLGWTMFELPSDAWLTELTRDYNDRSRISGWQGLAAQAGGFVFFA
                     ASVTGIFGKGMSAELLRAVGWASLIILPGAVVLAAIVVPSGAQSAKAFETPKLSGLWQ
                     ALRSNGALQVLLSSQLLGGFGGGIYLGTQLLLLDGYFHQGHNFGLIFMGYQVVHFAAM
                     PVWLKIIYRFGKHRSWALSWGLSAAMTPLSLLVPPGENAIVPLLILAMIRSAVGGADI
                     VAPRALMADAIDYGILKTGENSAGSYFAASSLAVKFCAAVSSGVAFWLLAMISYDPKP
                     GAVNTSSAIVGLIVVGMLLPMICNVLAASLIWRFPIDRRRASIIRRRVEGRRSHAAPA
                     E"
     gene            complement(3125..5344)
                     /locus_tag="PLO65_00175"
     CDS             complement(3125..5344)
                     /locus_tag="PLO65_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26693.1"
                     /translation="MQNLLLGVAAGALLATPALAQSATTDTTVDSLIVTANKREQSLL
                     TVPGSIAALGEDAMDDLMVNDVQDYADLIPGLDITAPVPGITKITIRGLSSESVTSSV
                     GVYINETPVTQNELDPDLKLYDVNRIEVLRGPQGTLYGEGSLGGTVKIVTNAPDLSGA
                     YGSFETSASQFENGDGLNYSAAGFFNAPLGENAAVRGVLSYRSNASWITNLGSGHADN
                     DEQTLTGRIALKLRLSEMADLTLTYIGQSIELGGPRNVINPALGDLKRSTLVDESVSD
                     DLSILSATLAWDLGFADLTSDTSYYTRTQNTVGNNNQTGGTRFFYNEISGAAAGFGPF
                     AQFGAPPGFPYVTTPESFVDFTGEAEQLTEEIRLVSKPGERFDWIVGAFYKDRHDTKD
                     TYFEGVITNSPVVDFSTLDIRRSRTLLTFDWQQIAVFGEVTVHLTPSLDFTAGLRYAS
                     EEVHNRENLDGLFQYNFGTYPPTSTPVQFGPVSDTFESTTPRFVLSYKMNDDWMAYAT
                     ASKGFRSGGFTGVTPFKPDSVWNYEVGLKGHTADNRFSIAGALYRIEWTDVQVFDDIA
                     TPPFFAVRNIGEAEVTGLELEATARPTERLELSAGLNLIDTEITAIDPSASDPDIIAA
                     KGNELQKVPNLSYSASGTYTWPVSEGWEGRATLSVVYQGESFSDFRNLASEELDSYTT
                     VATRIGVESDRYSVYLFGRNLTDERIQLDISGPGSAVVGTTVGRPRVIGLEFKAKFN"
     gene            5546..6517
                     /locus_tag="PLO65_00180"
     CDS             5546..6517
                     /locus_tag="PLO65_00180"
                     /inference="COORDINATES: protein motif:HMM:NF004793.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRD26694.1"
                     /translation="MMHIGTEAVAATAGYATLVDAIAEAFRGGVTAPVRGHYSVPVPG
                     APDATLLTMPAWTEGGYLGVKIATVFPGNSQAGRRAVSGQYLLLDASDGQPLALIEGG
                     ELTARRTAAASALAASFLARTDATTLTMIGAGRLSAHLVRAHLALRPSLREVALWGRN
                     ADKAATVAARLRSELPGLHVSATDDLEASVRRSDLVSAATLSTDPIVRGAWLRPGVHV
                     DLVGGFTPRMREADDEAIRRARVFCDTLEGAPQEAGDLCQPIASGLLDPGSLTSLTQL
                     CRQERPGRLEAGDITLFKSVGCAAEDLAAAVVIYEAQKKVLNASTTS"
     gene            complement(6481..7773)
                     /locus_tag="PLO65_00185"
     CDS             complement(6481..7773)
                     /locus_tag="PLO65_00185"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRD26695.1"
                     /translation="MDIDWIRALPVDSTTKGMPGGLEAMPQSQVGEMGWNVLRQDLPF
                     PLAVLKREALDANRTWMRAFTAMAGIMIAPHGKTTMTPEIFALQMADGAFGITCATAG
                     HLEVYRAAGVPRVILANQLVGRTNLRMALAHLRDPAFDLYCLVDSEAGLGAIAETARE
                     LGLDRPVKVLIEIGAAGARTGVRTIEEGVALARLAVALPEVRLAGVETYESIFPEAEK
                     TDPERPVAGLLDLAAMAAERIAAELPPSAEPFLLTAGGSDFFDLVVERFRRLDLGRPV
                     EIVIRSGCYITHDSLHYADCIKRLIARSPGAGALPGALQPALEVWGAVQSRPEAGRAY
                     INLGKRDISYDWELPTPTSWYRPGLHSAPQSSPAGWRTLRLNDQHLHLEVPADDGIRV
                     GDLVAFGISHPCTTFDKWRVIPVVNAGYDVVDAFRTFF"
     gene            complement(7776..9992)
                     /locus_tag="PLO65_00190"
     CDS             complement(7776..9992)
                     /locus_tag="PLO65_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD26696.1"
                     /translation="MTAVTGLPNLPVEAYQKAEALLSWNAVKLVRNASVSPIWETDSR
                     GFKYRRQEADGTEQWVRIDAASGERTPASPPAKTDQPWRDGELPSPDRRYALRLDNHN
                     ILVRDIASGDERPLTTDGVPLHGWGGSPGTNLEPVTQTRSGRHPLPVAAWSPDSRRVV
                     AHRIDERGLKDFHFLEACPKDGAALPKLYSVKFSTPDDAVLATAQLAIFDIETGASRP
                     IQLPPELITRESPIERAQVWWSASGAVLFVMVVDRGDKAFHLHRVDPATGAATLLWTE
                     ASQTYVEANVGWNEPIGRVLDDGGKLLLMSERSGWMHLWLHDGLTGAPLVQLTSGDWV
                     VREVVRVDEANGRVFFLAGGREPDRDPYLQHLYSVRLDGSDLTLLTPEPAEHRVAVWS
                     PPPFLTWRGYPDFNAPRAEGVSPDCRYVVETYSTVTTSPVSVLRRTADGSVVTVLETA
                     DVAALEEAGWTWPVPFRVKAADGKTDIYGALWLPRGYDAAGSWPVIDSIYPGPQTIRT
                     PKAGVSGDLWGMGSLAGGAAMAMLGFAVVTIDATGGPFRSKAFHDISYGRMERAGGLE
                     DHMAAIRQLAERYPGLDLDRVGIFGFSGGGTATCRALFEYPGFFKAGVAAAGSHDLLG
                     YTAYWGEKYEGLFEAESYRRVRNGNLAANFRGKILLVAGELDDNCHPAMTLQVAQALM
                     KAHKDFELLIMPGHNHMTPGGAGYFTRRLWDFFVRHLNGLEPPPHYVLKGPLDEVV"
     gene            complement(9989..11530)
                     /locus_tag="PLO65_00195"
     CDS             complement(9989..11530)
                     /locus_tag="PLO65_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD26697.1"
                     /translation="MRQSDAANAIIAGLSRRDAPGGVAGVVVNGEMVWRGAFGLADLS
                     TGAVFAPDTRFHVCSITKTFTALLLGLLEADGALSMDDPLRRHLPSTPDYDTPLTLRH
                     LVTNSSGLRDYFPLMWLEAGRMVGAYPREPMERHALAQPTLMFPPGSRYSYSNSNFVA
                     LCRVMESLTGQTYATLARERILEPLGMARSEFRTQTIPEPAESALGYFEDGDGGFRAP
                     RLDVHEAGDGGLWSTLDDMARYGAALASGAFGPPDLIARLTAPTLLTSGHSSWYGSGF
                     GTGERGGARWFGHAGGLAGMSTNLACFPDHKAAVITAFNGPAGDAEELGFRLADIFLD
                     LRPAPRPHAGKTLPATDWAGCWQDERAGLTAEISVNGERGELTQFGWTVPLSATTPDV
                     LEDPESATIVRRLGKDSLSLAAGRGAPVTLTRRPAGAARAPTGVFQGLEVETRLEIRD
                     EGAVLYGRDGPSPVYPLKAAARDVWTLLRPEGGRAGVVITRTPDGSLLVSMAKAERIL
                     FRRLP"
     gene            11666..12919
                     /locus_tag="PLO65_00200"
     CDS             11666..12919
                     /locus_tag="PLO65_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HRD26698.1"
                     /translation="MELTADENSWLEGACGPGVAMAMRILVDMGRILGADRLIPIASS
                     HIDGCLYHGPAGVDFAERLVEGRAQVVVPSTLNVGAVDLRRPDRMLLSAEDHDLALRL
                     MRAYEALGCKPTWTCAPYQAGHRPAVGQDIAWAESNAVVFVNSVLGARTNRYGDFMDI
                     CCAVTGRAPRYGLHIPENRAARLVVTTDGLSDRLKAMEAFYPVLGAWLGRESGGEIAV
                     IDGLPSSTTEDQLKGLGAAAASTGAVALFHVAGVTPEAPTLEAALQGRPPARVVALTP
                     DMVRTARDSLSTVAGERLDVVALGSPHFSEAEIDAFEAALAGRATRAPVYICTGRHVA
                     DRLAEAGRLEALEARGVEIVIDTCVVATPILKRGPGGVLMTNSGKFAHYGPANTGYES
                     VFGTLGDCVESAVAGRVVRDERAWS"
     gene            12916..13314
                     /locus_tag="PLO65_00205"
     CDS             12916..13314
                     /locus_tag="PLO65_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HRD26699.1"
                     /translation="MNGEVIVAGEGSGEVLHLTRPISFWGGVDPKDGRVTDPRHPQAG
                     QSMTGRVVVIERLIGSSSGSSVLLELVARGAGPAALVLGEVDAITALGAIVAREMGHA
                     ATPIIQLDSRLFAGLPRRCQVTPEGSILAV"
     gene            13382..14479
                     /locus_tag="PLO65_00210"
     CDS             13382..14479
                     /locus_tag="PLO65_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRD26700.1"
                     /translation="MALLKADPSRLPDVAAADVTHFVRQADRPQAGRLDEAQPLADGV
                     GARYQLDGVEGDGYREFIRASDDLFVVMVDTTANIDWTLKFVEEDFLTFQFRLEGAST
                     EMAAEQEEPMEPDATFFGVDLHPAGMEKIVWIPSGTRFRQVSLKLKPSFVQRLLGGPP
                     DWMPEPPRAYLDGGAAEFFSLRLPLSGAMRQSCADLLDCGFQGALRRAYAEAKAGELL
                     CLALDAMARRDETRVLPVRLYERDIVRLREAQEILLENYAAPPPVTRLARRVGLNRNK
                     LAYGFKHIFGVTISQFLQMKRMEEAYALLKEGRMGVAQAAETVGYADPGGFAKLFRRH
                     FGVLPKDVLQDGVNKRPESAGRDARPSQSEP"
     gene            14538..15548
                     /locus_tag="PLO65_00215"
     CDS             14538..15548
                     /locus_tag="PLO65_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HRD26701.1"
                     /translation="MSEVSLETPYLLFLGDVQDRILAKTAAGVAHWRPDICVGEVALP
                     GCAVSLGLPRVSPAEGAALGARTLVVGVANAGGYISGAWTGLLLEALEAGLNIASGLH
                     GRIADVPVLAEAARRLGRRIDDIRHPREAPPIGRGSRRGGRRLLTVGTDCAVGKMYAS
                     LAVARELNARGVSARFRATGQTGIFIAGEGVCVDAVVSDFISGSVEALSPAIPADQWD
                     VIEGQGSLFHPAYAGVTLGLIHGSQPDAIVVCHEAGRERLHEFEDFATPSLPLAIARN
                     LEAARLTNPDVRCVGISMNTSRLSADKAEALMARTSRELGLPCVDPVRTGVSALVDRL
                     LA"
     gene            complement(15545..16651)
                     /locus_tag="PLO65_00220"
     CDS             complement(15545..16651)
                     /locus_tag="PLO65_00220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD26702.1"
                     /translation="MAIVGGGIAGVALAWRLAARFSVVVLEREDGLGFHATGRSAAEW
                     SLVHAEGLTRVLTAASAPFLEAPPAGFADQPLVRRRGNVVYVRPGGLEAFADLLAHGR
                     TLQQDLQEISPREAAGRAPFLDPDRLSAAFWDPLNGEIDVDALLTGCQRAARRAGAVF
                     RTGVTLHGAERQADRWRLATSDGPVDADLLVNAAGPWADAVARQCGADPLGLQARRRT
                     AITFDPAEGLDIRGLPSADDAESGFYIKPEGGRLMACPGDATPSEPCDARPEEIDVAT
                     AAWMAEEVTGRPVRRLGATWAGLRTYAPDDQPVAGWDPKVRDLFWLAGLGGAGLMTSP
                     ALSAAAAAILETGDTPPALARDGITAVALSPARL"
     gene            16804..18900
                     /locus_tag="PLO65_00225"
     CDS             16804..18900
                     /locus_tag="PLO65_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRD26703.1"
                     /translation="MEVSFASEAAQLRLGAGETFRGEGVLAVTKALLQSGVAYVGGYQ
                     GAPVSHLLDVMVQSRDLLDELGVHLETCANEAAAGALLGASIAYPLRGAVTWKSVVGT
                     NVASDALSNLASAGVTGGVLIILGEDYGEGASVIQERSHAFALKSSVWLLDPRPDTAR
                     LVDMVEHAFALSEASNTPVMMELRIRACHRYGVFTTKDNVRPAHAGAARPIAPAPFDY
                     GRLSHPPSTFLQEKDKLSRRLPAALDYIRDHRLNDLIDGDIADVGLIVQGGLFNTVLP
                     ELARLGFADRGGASRLPILVLNVVHPIAPEEIAGFCRDKRAVLIFEEGAPDYIEQFIG
                     ATLRRAGLSTALHGKDLLPPAGEYTAEAVRAGLRGFLARASLEAVDDGWEASVATARA
                     KATDALGGVPDRPPSFCVGCPERPFFTALKLVQKDTGPLHVAADIGCHALATFPPFDS
                     GQTILGYGMSLASSQAVAPFQARRPLAVMGDGGFWHNGLITGVAGGAFNQADQILVVL
                     DNGYSSATGIQDIPSSRKGLAASIEAAVRAVGVRWVRKALTYRVADMVALLREAVTTA
                     ETGLKVIVAEGECMLARQKRERSALTARLARGERVVRIRYGVDEAICTGDHACIRLSG
                     CPSLTLRPRSDPLRNDPVAHVDNDCVGCGLCGEVAHAAQLCPSFYRAELVRNPRRWER
                     WLHRVGVWASGAAVAA"
     gene            18897..20384
                     /locus_tag="PLO65_00230"
     CDS             18897..20384
                     /locus_tag="PLO65_00230"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HRD26704.1"
                     /translation="MSSPDPITVLICALGGEGGGLLAQWITRAATLEGLAVSATSVPG
                     VAQRTGATSYYLEIQPGGGGEAVMGLHPVPGRIDILVASELVEAGRAMLRGFVTPDRT
                     RLIASTHRVFTISERAAPDDGRVDGDRILSAARDMARETLLFDAATLAGAHGSRSNAV
                     LLGALAATGALPISAASFEAALDANGSGGAANLAGFSAGMAAAENRDAPLRRPDDPQP
                     PATDLERELHGLPAGARETAAHGVARLIDYQGRRHAKAYLDRVGRIARLDADGALTEA
                     TARQLALLMSQEDVIRVAQLKIDPRRLARVRREAGAAPGDPCRIHEFLKPGLAEAVAL
                     LPAAAGRFLSARIAASPRLSALAWSLKIETTAVWGYLLLRGLAALRPLRPLSFIHADR
                     EALVDRWLGLVERAAPLSTALAREVVEAASLVKGYASTLERTRGAFFRVLDAVVEPAV
                     AGDLPEAHAADAVLQARLAARRDDEGRALSALIAALPRVGRDIAA"
     gene            20381..21310
                     /locus_tag="PLO65_00235"
     CDS             20381..21310
                     /locus_tag="PLO65_00235"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRD26705.1"
                     /translation="MNLEDRDGLIWLDGALIPWREARVHVLSQTLHHGMGAFEGVRAY
                     DGESGVAVFRLREHTRRLFNSCKIVGLTLPWTPEQIDEACLAVIRANGYGGCYLRPVV
                     FLGGENPGVSAAGNSLHAAVIAWPWEDYLGAGAAQRGVRLKVSSFSRHHHNAVLLKAK
                     ANGLYINSMLANQEARSLGFDDALMLDVSGFVAEASTSNIFVVRDGEIATPPRTSVLE
                     GITRASVITLLADLGHPVVERHLTRDELYIADEAFLTGTASELTPVSSVDDRPLHACP
                     GPVTLQVQAAFHAAVRGRSRDRLAWLTPVGPGQ"
     gene            21307..22488
                     /gene="pobA"
                     /locus_tag="PLO65_00240"
     CDS             21307..22488
                     /gene="pobA"
                     /locus_tag="PLO65_00240"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRD26706.1"
                     /translation="MSGRTQVVIVGAGPAGLFLGHLLHRAGIETVVLEQRSRDYVEGR
                     VRAGVLEQVTIDLMARLGLDARMRREGLVHSGTMIACDGDLFRIDMEALTGGSTIMVY
                     GQSEVMKDLGDGAAARGLRVVYDAEAVTLHDIDSDRPTVTWRQAGEEQRLACDFIAGC
                     DGFHGVCRASIPGADQATFERVYPFGWLGVLAEVPPCEDELIYANHERGFALASMRSP
                     TRSRYYVQCSLADRVEDWPDERFWDEVCLRLGRSAAATVTRGPSFEKSIAPLRSFVCE
                     AMRHGRLFLAGDAAHIVPPTGAKGLNLAASDVIYLAEALIAHCQGGDDRALDRYAPRA
                     LARVWKAERFSWWFTGLTHRFPDTDPFDRRIQLAELAYLRGSRAAQTTLAENYVGLPL
                     S"
     gene            22494..23207
                     /locus_tag="PLO65_00245"
     CDS             22494..23207
                     /locus_tag="PLO65_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRD26707.1"
                     /translation="MSGKIFANADKALKGFLFDGMTLMSGGFGLVGIPEDLIAAVRRS
                     GVRDLTIISNNCGAQGVGLWTLLENRQIRKMVSSYIGGNRLFAERLEAGDVELELAPQ
                     GTLAERVRAGGAGIPAFYTRTGVGTIVAEGKPSEIFEGHTYIRERWLRADLSLVRAWK
                     ADPAGNLQFRKTARNFNPVMATAGRVTVAEVEEIVPAGGLDPDSVHTPGIYVDRVIRS
                     HAPKRVENLTTRPRPAETA"
     gene            23207..23848
                     /locus_tag="PLO65_00250"
     CDS             23207..23848
                     /locus_tag="PLO65_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRD26708.1"
                     /translation="MAWTRDEMAARAARELVSGQYVNLGIGIPTLVANHIPPDVEVIL
                     QSENGMLGMGPFPFEDEVDPDLVNAGKQTVSQLPTSSFFSSADSFAMIRGGHVDLTIL
                     GAMEVSQAGDIANWSVPGETLKGMGGAMDLVAGVRRVVVIMDHTSRAGRPKILRRCAL
                     PLTGAACVDLIVTDLAVFERRGGGLVLIELAPDVSLDEVRAKTDADFICGALS"
     gene            23845..24543
                     /gene="pcaH"
                     /locus_tag="PLO65_00255"
     CDS             23845..24543
                     /gene="pcaH"
                     /locus_tag="PLO65_00255"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HRD26709.1"
                     /translation="MTGPISFRREDAGQPALLYPPYRSTLLRTPREASVRIPQTVTET
                     TGPTQWTRLMGAAMADLTTQHAAPPIGQRIIVAGRVLDDRRRPVPRTVMEIWQANAAG
                     RYIHKKDQWDAPLDPNFTGAGRVITDDEGRFRFVTIRPGAYPWGNHPNAWRPAHIHLS
                     LLGPAFATRLTTQLYFPDDPLIALDPIAGAVPAPFVDRLVARFDLDLTEPSWALGYRF
                     DVVLRGHDATPMEA"
     gene            24546..25190
                     /gene="pcaG"
                     /locus_tag="PLO65_00260"
     CDS             24546..25190
                     /gene="pcaG"
                     /locus_tag="PLO65_00260"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HRD26710.1"
                     /translation="MPTPPQRPDLFGQTPWQTVGPFFHYGLPWKGGADLVGASDLGAR
                     ADLVPEAHYLLAGPASRGEIAGEIIEIVGRVTDGAGDPLPDALVEIWQADASGRYNTD
                     DPEGFIGFGRAATDDDGAYRFRTLKPGPAPGPDGRAQAPHIAFGISGRGLLKRLVTRL
                     YFGGEADNADDPVLSLVPEPRRQTLMATAVAASAHSWRFDIVLQGEGETVFFDF"
     gene            25202..26356
                     /gene="pcaB"
                     /locus_tag="PLO65_00265"
     CDS             25202..26356
                     /gene="pcaB"
                     /locus_tag="PLO65_00265"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HRD26711.1"
                     /translation="MTSLIRDLAASTPEMIDVFGDAATVRAALRFEAALAGACAAEGL
                     VSPEAARAVICACSKPPDLESLAREAAHAGVLAIPLVRWLRTTADTVSPGAGDAVHLG
                     ATSQDLADTAMVLQAREAARLVDGDLARLADSLADLAETHAATPMIGRTLLQQALPVT
                     FGLKAAHWMMSIEGVRARLRREADSAMMLQFGGPAGTLGGLAGKGDAVARRMATELGL
                     GCPLLPWHSRREAIAGLASALAIVVGAIAKIALDVALLAQVEVGEVSIPRQGDRGGSS
                     AMPHKRNPTGSQIALSAAHRAPGLATSILAGMPQAHERGLGGWQAETPVLVELFLITH
                     GAVVAVAGQVDGLDVHTDAMRRTLDAAGLGQDIGESLDMVRRALANREIR"
     gene            26356..27552
                     /gene="pcaD"
                     /locus_tag="PLO65_00270"
     CDS             26356..27552
                     /gene="pcaD"
                     /locus_tag="PLO65_00270"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HRD26712.1"
                     /translation="MPFANHAGARLYWRQEGSPDKPALLLLSSIGTDQSLWDLAAPHL
                     LDDFRILRMDTRGHGASDAPTGPYSLAQLAGDALSVLDAAGADQSTICGVSLGGMIAL
                     RLALDAPERVAGLAIACTSAEMDRAAWQARIDTVTAEGTGAIADMAMQRFFPEAFRRS
                     HPRVVDTVRGGLLATDPTGYAGCCAAIRDMDILSRLGAIAAPTLVIHGLRDISTPFVG
                     HGDRIAAAIPGARSAALDTGHLPCLEAPGGFAALVRRFHQRTVHGNVEDDAADALYAA
                     GLRTRRKVLGDAWVDASLESRTDFNADFQAMITRCAWREVWSRPGLDHRTRRLLVVAM
                     TASLGRWEEFRLHVRSGLDAGGFTVDELKEVLMQVAIYAGVPAANTAFAEAGGIVESW
                     RARFGA"
     gene            27658..>27764
                     /locus_tag="PLO65_00275"
     CDS             27658..>27764
                     /locus_tag="PLO65_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD26713.1"
                     /translation="MKIAFIGLGVMGFPMAGYLAKAGHEVSVFNRGPAKA"
BASE COUNT         4257 a         9569 c         9603 g         4335 t
ORIGIN      
        1 cggtggccgg cgcgctgggc tgggcgaagg cggccgggcc gagcaccgtg gaccagcccg
       61 ctgcacgcgc ccgcgcgagc agctcgccat acccgacgac gctggcgagg ccggagtcac
      121 cagcgccggg gctctccgac ggtctcagcc acaggctgtc ctcgaagctc gcccgtccgg
      181 tcgggatcgg cgagaaactg ttggtttcgt gctggcacgc cgccaggtag acacgccggc
      241 tcatcacccg gcccgctcga agacgccgat cccgtggatc gtcagggacc gtacctggtc
      301 tcccgccaga tcgaattccg cctcgaaccg accgcccacg gcatcgctga ccagccccga
      361 atagtccggc accgcgagaa accggtctcc gcccaacggc gccagttcgc catcgaagac
      421 cggcgcgtcg aggaaacgga gcgacagccg ttccgcctgc acggcgacat ccagcgcccc
      481 ttcgcgacgg ctgtggaatc ggccggcata ggtgtcccag ggccgatccg ggcgggcgcc
      541 attcgggcgg agggtcgagt gcttgaagcc cgcgttcgta tccggcagtt tccggtcgaa
      601 gatgacgtcc cgccagtcac ccgggctcca tcccatcagg gcgtccagga ccgcgtagct
      661 gatttcgatc gaggccttgc agtccttgtt gacgtagacc gccacgccgg acctttcggc
      721 gggcgccagg gcgacatggg cgttgatgcc gaaatccgaa ccggagtgcc ggatgacccg
      781 gacaccgttc tgccaggcga cgccccagcc gagcgcgtag gcgggtccgg ttgcgccggg
      841 tggcgcgatc cagatggcga gctcttcctc gcgcagcgcg atctgcggct cgaacagttc
      901 ggccaggccc gacgccggca ggatgggcga accgtctccg aggttgaagg cgagccagcg
      961 tgcggcatcc tccgctgaaa ggcacagccc ggcagatccc cggtggacgt cggtaaagat
     1021 cggcgggatt tcgaccgtct ccccgtccag cgcgcagtgc cagcccgcgg ggtccttgag
     1081 atccgtctgc gcgaccgccc cgccggatgc gtccttcatc cccagcgggc cgaagatctc
     1141 ccgcttgaga aagtcgacgt aggcgaggcc gctggcccgc tccacggcga gcgcgaccgc
     1201 cgtatgggcg atgttcgaat aggtgaagcg ctcccgaaga cccgcggcgg gcggggaaaa
     1261 gcgcagccgt cggaccacct cctcggccgt gatggtcagc tccgtgccga actcgagaat
     1321 gccgttgcgc ggcagcccgg cccgattgcc cgacaggtcg cgcagggtca tggcggcggc
     1381 gtcggactct cgccaggtcg tgaaatccgg aagccaccgg cgtaccgggt cgtcccagtt
     1441 catgatcccg cgcgcgacga gcaccgccac ggcggtcgcc gaaaaggcct tggaggtgga
     1501 gccgacgttg aacgccgtgg ttgtgcccac ggggtccgga agggccaggt ggcgccgacc
     1561 gaatcccgat gcgaaggcct gccccttcga aaccagggcc acagcggccc ccggcgcgtc
     1621 ataggccgca aggatggcgt tgatgcgcgc gtccaggtcg ccgctgaaga cgctgacaga
     1681 tgggaacgcc gacattgcct actccgccgg cgcggcgtgc gaacgccggc cttcgacccg
     1741 ccgccggatg atgctggccc gacgccggtc gatagggaac cgccagatca gcgacgcggc
     1801 gagcacgttg cagatcatcg gcagcagcat gccgaccacg atcagaccga cgatggcgct
     1861 gctggtgttc accgcgccgg gcttgggatc gtagctgatc atggccagca gccagaaggc
     1921 cacgccactc gacacggcgg cgcagaactt caccgccagc gacgaagccg cgaagtagct
     1981 gcccgccgag ttttcgcctg tcttgaggat gccgtagtcg atggcgtcgg ccatcagggc
     2041 gcgcggcgcg acgatatcgg cgccccccac ggcagagcgg atcatcgcga ggatcaacag
     2101 cggcacgatg gcgttctcgc cgggcggcac gagaaggctc agcggcgtca tggccgcaga
     2161 gaggccccag ctgagcgccc acgaacggtg cttgccgaac cggtagatga tcttgagcca
     2221 gaccggcatg gccgcgaaat gaacgacctg gtagcccatg aagatcaggc cgaagttgtg
     2281 gccctggtgg aaatagccat ccaggagcag aagctgggtt ccgaggtaga tgccgccccc
     2341 gaagccaccc agcagctgcg aggagagcag cacctggagc gcgccattgc tgcgcagggc
     2401 ttgccagagc ccactgagtt tgggggtttc gaacgccttt gccgattgtg cgccggaagg
     2461 cacgacgatg gcggcgagaa cgaccgctcc gggcaggatg atcaggctgg cccagccaac
     2521 ggcccgcagg agttcggcgc tcattccctt gccgaagatc ccggtgactg acgcggcgaa
     2581 aaagacaaag ccgccggcct gggcggcaag accctgccag ccggaaatgc gcgagcggtc
     2641 gttgtagtcg cgggtgagct cggtcagcca ggcgtctgat ggcagctcga acatggtcca
     2701 gccgagatag aagatcagcg accagagcag gaagtacgtg acggtggcgt cgccgcccgg
     2761 ctgaaagagg aagaagatcg aggcggcgat caggatcgcg ccgccgacga tccagggctt
     2821 gcgagcgccc cagggcgact tggtcttgtc gctcagccat ccgaccaggg gatcggtgaa
     2881 ggcgtcgaac agacgcgatg cgatcagtgc ggtgcccagc cccgccagcg tggccttggt
     2941 gttctgggcg tagaaggtcg ggaggacata aaagaatggc gccgtgatca ggcccagcgc
     3001 cagtttgggg agaacgaagg cggcaagctg cccgatgggt acgcgagagg tcatcacaag
     3061 gtcctcggca tcagggctat ggaacggtgc ggcgggggtg gccgtccacc cccgcctggc
     3121 cgtgtcagtt gaacttcgcc ttgaactcga ggccgatcac ccgcgggcga ccgacggtgg
     3181 tcccgacgac ggcgctgccg ggtccggaga tgtccagctg aatccgctcg tcggtcagat
     3241 tgcgaccgaa gaggtagacg ctgtaccggt cgctctcgac accgatgcgg gtcgcgacgg
     3301 tcgtgtagga gtcgagttcc tcggaggcca ggttgcggaa gtccgagaac gattccccct
     3361 ggtagaccac gctcagggtg gcccgccctt cccagccttc ggagaccggc caggtgtagg
     3421 tgccggacgc agagtagctg aggttgggaa ccttctgcag ctcgttaccc ttggcggcga
     3481 tgatgtcggg atcgctggcg ctcgggtcga tcgccgtgat ctcggtgtcg atcaggttga
     3541 ggcccgccga cagttccagc cgctccgtcg gcctggccgt ggcctcgagc tcgagacccg
     3601 tgacctcggc ctcgccgatg ttgcgcaccg cgaagaaggg cggcgtggcg atgtcgtcga
     3661 acacctgcac gtcggtccat tcgatccggt agagcgcgcc ggcgatcgag aacctgttgt
     3721 ccgccgtgtg ccccttgagg ccgacttcat agttccagac gctgtccggc ttgaacggcg
     3781 tcaccccggt gaagccgccc gagcggaacc ccttcgaggc cgtcgcatag gccatccagt
     3841 cgtcgttcat cttgtaggac aggacgaacc gcggcgtcgt cgattcaaac gtgtccgaga
     3901 ccgggccgaa ctggaccggc gtcgacgtcg gaggataggt gccgaagttg tactggaaga
     3961 gcccgtcgag gttctcgcgg ttgtgcacct cttcggaggc gtaccggagg ccggccgtga
     4021 aatcgaggct gggcgtcagg tgcaccgtca cctcgccgaa caccgcgatc tgctgccagt
     4081 cgaaggtcag cagggtgcgg ctgcggcgga tatccagggt ggagaaatcg acgaccggcg
     4141 agttggtgat cacaccctcg aagtaggtgt ccttcgtatc gtgccggtcc ttgtagaagg
     4201 cgcccacgat ccagtcgaaa cgctcgcccg gcttcgacac cagacggatt tcctcggtca
     4261 gctgctccgc ctcgccagtg aaatccacga acgattccgg tgtcgtgaca tacgggaatc
     4321 cgggaggcgc gccgaactgc gcgaacggtc cgaagccggc cgcagcgccg ctgatctcgt
     4381 tgtagaagaa gcgcgtgccg ccggtctggt tgttgttgcc gaccgtgttc tgggtccggg
     4441 tgtagtagga cgtgtcggag gtcaggtcag cgaagcccag gtcccaggcc agggtggcgc
     4501 tgaggatgct gaggtcgtca gaaacggact cgtcgaccag cgtgctccgc ttcaggtcgc
     4561 cgagggccgg attgatcaca ttgcgcggcc cgcccagctc gatgctctgg ccgatgtagg
     4621 tgagcgtgag gtcggccatt tcggacagcc gcagtttcag cgcgatcctg ccggtcaggg
     4681 tctgttcatc gttgtcggcg tgccccgatc cgagattggt gatccaggac gcgttgctgc
     4741 gatagctgag cacgccgcga acagcggcgt tctcgcccag cggcgcgttg aagaagccgg
     4801 cggcggagta gttcaggcca tcgccgttct cgaactgcga agccgaggtc tcgaaggacc
     4861 cgtaggcgcc ggacaggtcg ggcgcattgg tgacgatctt gaccgtgccg cccagcgacc
     4921 cttcgccgta gagcgtccct tgcgggccgc gcagcacctc gatcctgttg acgtcgtaga
     4981 gcttgagatc cggatcgagc tcgttctgcg tcaccggcgt ctcgttgatg tagacgccga
     5041 cgctgctggt caccgactcc gacgacagtc cgcggatcgt gatcttggta atgcccggca
     5101 cgggcgccgt gatgtcgaga cccgggatca ggtccgcata gtcctggaca tcattgacca
     5161 tcaggtcgtc catggcgtcc tcgccgagcg cggcgatgct ccctgggacc gtcagcagcg
     5221 attgctcgcg cttgttggcc gttacgatca gagaatccac cgtcgtgtcg gtcgtggcgc
     5281 tctgggcgag cgcgggcgtc gccagaagcg cgccggccgc cacgccaagc aacagatttt
     5341 gcaagcggta tgacatgttg aaccaccccc tctttcgccc ttcaggattt cctgttccgg
     5401 gcgcgctgac catcatggtc tgcacccaga gcgtatgccc caagggcaaa tgtgaaacgg
     5461 cggcgcgtca cggccggccg tcccgatgtc actttgcccc gggatttctc cccctcaccg
     5521 gacatctcgg ctagaccagc cggacatgat gcacatcggc accgaagcgg tcgcggcgac
     5581 cgcaggctac gcaacgctcg ttgacgcgat cgcggaggcg ttccgcggcg gcgtcaccgc
     5641 gcccgtgcgc ggccactaca gcgtgcccgt tccgggcgca cccgacgcga ccctgctgac
     5701 catgccggcc tggaccgaag gcggctatct cggggtcaag atcgcgaccg tctttcccgg
     5761 caacagccag gctggacgga gggccgtttc gggccagtat ctgttgctcg acgccagtga
     5821 tggccagccc ctggccctta tcgagggggg agagctgacc gcccggcgga cggcggccgc
     5881 ctccgcgctg gcggcgtcct ttctggcaag gacggacgca accaccctga cgatgatcgg
     5941 cgccggccgg ctgtccgcgc acctggtcag ggctcacctg gccttgcgac cgtctcttcg
     6001 cgaggtggcg ctgtgggggc gaaatgccga caaggccgcg accgtggcgg cgcgacttcg
     6061 gtcggaactc cccggcctgc atgtcagcgc gacagacgac ctggaagcct cggtccgccg
     6121 gtccgatctg gtgagcgcgg cgaccctgtc gacggacccg atcgttcggg gcgcctggct
     6181 gcgtccgggc gtccatgtcg acctcgtggg tggattcacg ccccggatgc gcgaagccga
     6241 cgacgaggcg atccggcgcg cacgggtgtt ctgcgacaca ctcgaaggcg ccccgcagga
     6301 ggccggagac ctgtgccagc cgattgcgtc cggcctgctt gatcccgggt cgctgaccag
     6361 cctgacgcag ctatgccgtc aggagcgccc gggtcgcctg gaagcggggg acatcaccct
     6421 gttcaagtcc gtcggttgcg ccgccgagga tctggccgcc gccgtcgtga tctacgaggc
     6481 tcagaaaaag gtcctgaacg cgtccaccac gtcatagccg gcgttcacca ccgggatcac
     6541 gcgccatttg tcgaaggtgg tgcaggggtg ggagatgcca aaggcgacca ggtcgccaac
     6601 ccggatcccg tcgtccgcgg gcacttcgag atgcagatgc tggtcattca gccgcaacgt
     6661 ccgccagccc gcgggcgaag actgcggggc gctgtgcagg ccaggccggt accagctggt
     6721 cggcgtcggc agctcccagt cataggagat gtcgcgcttg ccgagattga tataggcgcg
     6781 gccggcttca ggccgcgact gcacagcccc ccagacctcg agcgccggct ggagggcgcc
     6841 cggcagggcg ccagcgcccg gcgaccgggc gatcagccgc ttgatgcagt ccgcgtaatg
     6901 caggctgtcg tgggtgatgt agcagccgct gcggatgacg atctccaccg gccggccgag
     6961 atcaaggcga cggaagcgct cgaccaccag atcgaagaag tccgatccgc cggcggtgag
     7021 gaggaacggc tccgccgacg gcgggagttc cgccgcgatc cgttccgcgg ccatcgccgc
     7081 caggtccagc agacccgcaa ccggccgctc cggatcggtc ttctccgcct ccggaaagat
     7141 gctctcgtag gtctccacac cggccagccg aacttccggg agggctaccg cgagacgggc
     7201 cagggcgacg ccttcctcga tcgttcgcac accggtccgc gcgcccgccg ccccgatctc
     7261 gatcagaacc ttgaccggcc ggtcgagacc gagctcacgg gccgtctcgg cgatggcgcc
     7321 aaggccggct tcggagtcga ccaggcaata gaggtcgaac gcagggtcgc gcagatgggc
     7381 cagggccata cgcaggttgg tccgcccgac cagctggttg gcgaggatga cgcgaggcac
     7441 cccggcggcg cgatagactt ccaggtgacc cgccgtcgca caggtgattc cgaatgcgcc
     7501 atcggccatc tgcagggcga aaatctctgg cgtcatggtg gtcttgccat ggggcgcgat
     7561 catgatcccc gccatcgccg taaaggcgcg catccaggtg cggttggcgt cgagcgcctc
     7621 gcgtttgagc acggcgagcg ggaacggcag gtcctgcctc aggacgttcc agcccatttc
     7681 tccgacctga ctctggggca tggcctcaag gccgccgggc atgcccttgg tggtcgaatc
     7741 caccggcagc gcgcggatcc agtcgatgtc catggtcaga ccacctcgtc cagcggaccc
     7801 ttgagcacat agtggggcgg cggctccagg ccgttgagat gccggacaaa gaagtcccac
     7861 aggcggcggg tgaaatagcc cgcgccaccg ggcgtcatgt ggttgtgtcc gggcatgatc
     7921 agcagctcga agtccttgtg cgccttcatc agggcctgag cgacctgcag ggtcatcgcc
     7981 gggtgacagt tgtcgtccag ctcacccgcc acaagcagga tcttcccccg gaagttggcc
     8041 gcaaggttgc cgttccggac ccgccggtag gactccgcct cgaacaggcc ttcgtacttc
     8101 tcgccccagt aggccgtgta gcccagcaga tcgtggctgc ccgccgccgc gacgcccgcc
     8161 ttgaagaatc cgggatattc gaacagggcc cggcaggtcg cggtgccgcc gccggagaag
     8221 ccgaagatgc ccacgcggtc gaggtcgagc ccggggtagc gctccgccag ctggcgaatg
     8281 gcggccatat ggtcctcaag cccccctgcc cgctccatcc ggccgtagga aatgtcgtga
     8341 aaggcctttg agcggaacgg ccccccggtc gcgtcgatgg tcaccacggc gaagccgagc
     8401 atggccatgg ccgcgccgcc cgccagcgat cccatgcccc acaggtcgcc agagacgccc
     8461 gccttgggcg tgcggatggt ctgggggccg ggatagatgc tgtcgatcac cggccaggag
     8521 ccggcggcgt catagccccg cggcagccac agggccccat agatatccgt cttcccgtca
     8581 gcggccttga cccggaaggg gaccggccag gtccagccgg cctcctccag ggccgcgacg
     8641 tcggcggtct cgaggacggt cacaaccgac ccgtccgccg tccggcggag cacggagacg
     8701 ggtgacgtgg tgaccgtcga gtaggtctcg accacatacc ggcagtccgg cgagacgccc
     8761 tcggcgcgcg gcgcattgaa atccggatat ccgcgccagg tcaggaaggg cggcggcgac
     8821 cagacagcga cgcggtgctc ggccggctcc ggcgtcagca gggtcaggtc cgatccgtcc
     8881 agccgcacgg aatagaggtg ctggaggtag gggtcgcgat caggctctcg acccccggcg
     8941 aggaagaaca cccggccatt ggcctcgtcg acgcggacga cctcccgcac cacccagtcg
     9001 ccggaggtga gctggaccag cggcgcgccg gtcaggccgt cgtgcagcca gaggtgcatc
     9061 cagccgctgc gctcggacat cagcagcagc ttgccgccgt cgtcgaggac ccggccgatc
     9121 ggttcgttcc agccgacgtt ggcctcgaca taggtctggc tcgcctcggt ccacagcagg
     9181 gtcgcggcgc ccgtggcagg atcgaccctg tgcagatgga aggccttgtc cccccggtcg
     9241 accaccatga cgaacagcac cgcgccggag gccgaccacc agacctgggc ccgctcgatc
     9301 ggcgactctc gcgtgatcag ctcgggcggc agctggatgg gccgcgacgc gccggtctcg
     9361 atgtcgaaga tggccagctg ggccgtggcg agcactgcgt cgtccggcgt cgagaacttc
     9421 accgaataga gcttcggcag cgccgcgccg tctttcggac aggcttcaag aaagtggaag
     9481 tccttcaggc cccgctcatc gatcctgtgg gcgacgacgc gccgggaatc cggcgaccag
     9541 gctgccacgg gaaggggatg ccgtcccgat cgggtctggg tcacaggttc gaggttcgtg
     9601 cccggggagc caccccagcc atgcagcgga acgccgtctg tggtcagcgg ccgctcgtcg
     9661 cccgaggcga tgtcgcgaac gaggatgttg tggttgtcca ggcgaagggc gtaccggcga
     9721 tccggtgacg gcaactcgcc atcccgccag ggctggtcgg tcttcgcggg cgggctggcc
     9781 ggcgtgcgct ctccgctggc ggcgtcgatc ctgacccatt gctccgtgcc gtcagcctcc
     9841 tgacggcgat atttgaaccc cctgctgtcg gtctcccaga tcggcgaaac ggacgcgttg
     9901 cggaccagct tcaccgcgtt ccaggacagc agcgcctcgg ccttctggta cgcctcgaca
     9961 ggcagattgg gcaggccggt cacggcggtc atggaagtct ccggaacaga atgcgttcgg
    10021 ccttggccat tgaaacaagc agggagccgt cgggggttcg ggtgatgacc accccggcgc
    10081 ggccgccttc cgggcgcagc aaggtccaga catcccgcgc cgcggccttg aggggataga
    10141 cgggcgatgg accgtcgcgg ccgtagagca ccgcgccctc gtcgcgaatc tccagccggg
    10201 tctcgacctc gagcccctga aagacccccg tcggcgcccg ggcggctccg gccggtcggc
    10261 gggtcagggt tacgggcgcc ccccggccgg ccgccagcga gagactgtcc ttccccagcc
    10321 gccgcacgat ggtcgcgctt tccggatcct ccaggacgtc cggggtggtg gccgacaagg
    10381 gcacggtcca gccgaactgg gtcagttcac cccgctcgcc gttcaccgag atttcggcgg
    10441 tcaggcccgc gcgctcgtcc tgccagcaac cggcccagtc cgtcgccggc agcgttttgc
    10501 cggcatgcgg ccgcggcgcc ggtcgcagat ccaggaagat gtccgccagc cggaagccaa
    10561 gctcctcggc gtcacccgcc ggcccgttga acgccgtgat cacggccgcc ttgtgatcgg
    10621 ggaagcaggc gaggttggtc gacatgcccg ccagtccgcc ggcatggccg aaccagcgcg
    10681 cgccgccgcg ctctcccgtg ccgaagccgc tgccgtacca gctcgaatga ccgctggtca
    10741 gcagggtggg cgccgtcagg cgagcgatca ggtccggcgg gccaaaggcg cccgacgcca
    10801 gcgccgcccc gtaccgggcc atgtcatcca gcgtgctcca cagaccgcca tcgcctgcct
    10861 cgtgaacgtc aaggcgaggc gcgcggaagc cgccgtcccc gtcctcgaag tagccgaggg
    10921 cggactccgc cggctcgggg atggtctggg tgcggaactc gctgcgggcc atgcccagcg
    10981 gctcgagaat tcgctcgcgc gcaagcgtcg cgtaggtctg gccggtgagg gactccatga
    11041 cccggcaaag cgccacgaag ttgctgttgg agtaggaata ccggctgccg ggcgggaaca
    11101 tcagggtcgg ctgggccagg gcgtgccgct ccatgggctc ccgcggatag gcgccgacca
    11161 ttcgcccggc ctccagccac atcagcggaa aatagtcccg tagtccgctg ctgttggtca
    11221 ccagatggcg cagggtcagg ggggtgtcgt agtccggcgt cgacggcagg tggcggcgca
    11281 gcggatcgtc catcgaaagg gcgccatcgg cctccagcag gcccagcagc agggcggtga
    11341 aggtctttgt gatcgagcag acatgaaagc gcgtgtccgg ggcgaacacg gcgccggtag
    11401 acagatcagc gagcccgaag gcgccccgcc agaccatctc gccattcacc acgacgccgg
    11461 cgacgccgcc aggcgcgtcg cggcggctca gtccggcgat gatggcgttg gcggcgtccg
    11521 actggcgcat tgagagcctc cccggggaca ccctgccgca agcaggggcg cgagcgtcaa
    11581 ggcggaaatc cccggttggc gggggattgt cactttgacg gaaagcgggg gatgaccgtc
    11641 cccaccgccg tgataggcca acggcatgga actgacagcg gacgaaaatt cctggctcga
    11701 gggcgcctgc gggccgggcg tggccatggc gatgcggatc ctcgtggaca tggggcgcat
    11761 tctcggcgcg gaccggctga ttccgatcgc ctcgagccac attgacggct gcctctacca
    11821 cggtccggcc ggcgtcgact tcgccgaacg gctggtcgaa gggcgggcgc aggtcgttgt
    11881 tccctccacg ctgaacgtcg gcgccgtcga tctccgacgc ccggaccgca tgctcctgtc
    11941 agcggaggac cacgacctgg ccctgcggct gatgcgcgcc tatgaggcgc tcggctgcaa
    12001 gccgacctgg acctgcgcgc cgtatcaggc cggccaccgg ccggccgtgg gccaggacat
    12061 cgcctgggcc gaaagcaatg ccgtcgtctt cgtcaattcc gtactcggcg cccggacgaa
    12121 ccggtacggc gacttcatgg acatctgctg cgcggtgacc ggccgcgcgc cgcgctatgg
    12181 tttgcacatt cccgagaacc gcgcggcgcg gctggtcgtg accacagacg gcctttcaga
    12241 tcgactgaag gcgatggagg ctttctatcc ggtgctcggc gcctggcttg gccgggaatc
    12301 cggcggggag atcgccgtca tcgacggcct tccttcctcg accacggagg atcagctcaa
    12361 gggactcggc gccgccgcgg cgtcaaccgg ggcggtggcc ctgttccacg tggccggcgt
    12421 gacccccgag gcgccgaccc tggaggccgc cctgcagggt cgaccgcccg ccagggtcgt
    12481 ggccctcacg cccgacatgg tccgcacggc gcgggacagc ctgtccacgg tcgctggcga
    12541 acggctggac gtcgtcgccc ttggcagccc gcacttctcg gaagcggaga tcgacgcctt
    12601 cgaggccgcg ctggccggtc gcgccacgcg cgcgccggtc tatatctgca ccgggcgcca
    12661 cgtcgcggac cggctggcgg aagcgggccg gctcgaagcc ctggaagcgc gcggcgtgga
    12721 aatcgtcatc gacacctgcg tggtcgccac gcccattctc aagcgcgggc cggggggggt
    12781 gctgatgacc aactccggca agttcgccca ctatggacca gccaacaccg gctacgagtc
    12841 cgtgttcggc accctcggcg actgcgtcga aagcgccgtc gccggacggg tcgtccgcga
    12901 cgagagggcc tggtcatgaa tggcgaggtg attgtcgcgg gcgagggatc gggcgaggtc
    12961 ctgcacctta cccgtccgat cagcttctgg ggcggggtcg atccgaagga tggccgcgtg
    13021 accgaccccc gccatccgca ggctggccag tcaatgaccg gacgggtcgt ggtgatcgag
    13081 cgcctgatcg ggtcaagctc cgggagttcg gtcctgctcg aactggtcgc gcggggcgcc
    13141 ggcccggccg cgctggtcct gggcgaggtc gatgcgatca ccgcccttgg cgcgatcgtc
    13201 gcccgcgaga tgggtcatgc cgcgacaccg atcatccagc tggattcgcg cctgtttgcc
    13261 gggctcccga ggcgatgcca ggtcacgccg gaggggagca tccttgctgt ttgaagtcgc
    13321 tgagctagca tcgggaccgg gatggcgcga cgggcgcggt cgtcctgacg gaggtggcgt
    13381 cgtggcgctg ctcaaggccg acccctcgcg actgccggat gtcgccgccg ctgacgtgac
    13441 ccactttgtc aggcaggcgg atcgccctca ggccggtcgc ctggacgagg ctcagcccct
    13501 cgccgatggc gtcggcgcgc gataccagct cgatggcgtc gagggcgatg gatatcgcga
    13561 gttcatccgg gcttccgacg atctgttcgt cgtcatggtc gacaccacgg ccaacatcga
    13621 ctggaccctg aagttcgtcg aggaggactt cctgaccttc cagttccggc ttgagggcgc
    13681 ttcgaccgag atggcggccg agcaggaaga gcccatggag cccgacgcca cgtttttcgg
    13741 cgtcgacctc catccggccg ggatggagaa gatcgtctgg attccgagcg ggacccggtt
    13801 ccgccaggtc tccctcaagc tgaagccgtc gttcgttcag cggctgctcg gcggcccgcc
    13861 ggactggatg cccgagcctc cccgcgccta tctggacggc ggcgcggccg agttcttcag
    13921 cctgcggctg ccgctttccg gcgccatgcg ccagtcctgc gccgacctgc tcgactgcgg
    13981 cttccagggc gcgttgcgac gggcctacgc cgaggccaag gcgggggagc tgctgtgcct
    14041 ggcgctggac gccatggcgc gtcgcgacga gacgcgcgtg cttcccgtcc gcctttatga
    14101 gcgggacatc gtccggctgc gcgaggccca ggagattctc ctggagaact acgccgcgcc
    14161 cccgccggtc acgcgtcttg cccgtcgcgt cgggctgaac cgcaacaagc tggcctacgg
    14221 tttcaaacac attttcggcg tgaccatttc ccagtttctc cagatgaaac ggatggagga
    14281 ggcctacgcc ctgctcaagg aaggccggat gggggtcgcc caggccgccg aaaccgttgg
    14341 gtatgcggac cccggcggct ttgcgaaact tttccggcgc catttcgggg tcctgccgaa
    14401 ggacgtgctg caggacggcg tcaacaagcg ccctgagtcg gccgggcgtg acgctcgccc
    14461 gtcccaaagt gagccctgac cctgtcgtca ggaagtgccg tggctagggt cgatctcgtg
    14521 ttcgcgaggt tacgaccatg tctgaagtca gtcttgaaac cccctatctg ctcttcctcg
    14581 gcgacgtgca ggaccggatt ctggccaaga cggcggcggg cgtagcccat tggcgaccgg
    14641 acatctgcgt cggcgaggtc gccctgccgg gttgcgccgt gagccttggc ctgccgaggg
    14701 tttctccagc cgaaggcgcg gcgctgggcg cgcggaccct ggtggtcggg gtcgccaacg
    14761 ccggcggcta catctccggg gcctggaccg gcctccttct ggaagcgctt gaggctggcc
    14821 tgaacatcgc cagcggcctg cacgggcgga tcgccgatgt tcccgttctg gccgaggccg
    14881 cgaggcgact gggtcgccgc atcgacgaca tacgccatcc ccgcgaggcg ccgcccatcg
    14941 gccggggcag ccgacgcggc ggacgccgcc tgctgaccgt cggcaccgac tgcgcggtcg
    15001 gcaagatgta cgcttccctg gccgtcgcca gggaattgaa cgcgcgcggg gtcagcgccc
    15061 ggttccgggc taccgggcag accggcatct tcatcgccgg cgagggcgtc tgtgtcgacg
    15121 cggtcgtgtc ggacttcatt tccgggtcgg tcgaagcgct gtcaccggcg ataccggccg
    15181 accagtggga cgtcatcgag ggtcagggct cgctgtttca tcccgcctac gcgggcgtca
    15241 ccctgggatt gatccatggc agccagcccg acgccatcgt cgtctgtcac gaggcggggc
    15301 gggagcgtct gcacgagttc gaggacttcg cgaccccttc attgcccctg gccatcgccc
    15361 gcaacctcga ggccgcccgc ctgaccaatc ccgacgtgcg ttgtgtcggg atctcgatga
    15421 acacctccag gctgtcggct gacaaggctg aagccttgat ggcccggacc tcgcgcgaac
    15481 tgggcctgcc ctgtgtcgac ccggtccgga ccggcgtgtc ggcgctggtg gaccggctcc
    15541 tggcctagag gcgcgcgggc gacaacgcca ccgcggtgat tccgtcccgg gcgagcgctg
    15601 gcggcgtgtc tccagtctcc aggatcgccg ccgctgccgc tgaaagggcc ggcgacgtca
    15661 tcaggccggc gccgccgagg cccgccagcc agaacagatc ccggaccttc ggatcccagc
    15721 cggcaaccgg ctggtcgtcc ggagcgtatg tgcgcagtcc ggcccaggtc gcgccaagcc
    15781 gacggacggg gcggccggtc acctcctcgg ccatccaggc ggccgtcgcc acgtcgattt
    15841 cctcgggtcg cgcatcgcag ggctccgacg gtgtggcgtc gccgggacag gccatgagcc
    15901 gcccaccctc gggcttgatg tagaagccgc tctcggcgtc atcggcggac gggagtcccc
    15961 gtatgtcgag cccctcggcc gggtcgaacg tgatcgccgt cctgcggcgg gcctgcaatc
    16021 ccaggggatc cgcgccacac tggcgcgcca cggcgtcggc ccacggaccc gcggcattga
    16081 ccagcaggtc ggcgtcgaca gggccatcag aggtcgccag ccgccaccgg tcagcctgac
    16141 gctccgcgcc gtgaagggtg acacccgtcc tgaacacggc tcccgctcgc cttgcggcgc
    16201 gctggcagcc ggtgagcagg gcgtcgacgt cgatctctcc gttcaagggg tcccagaagg
    16261 ccgccgaaag ccggtccggg tcgagaaacg gggcgcggcc cgccgcttcg cgaggcgaga
    16321 tttcctggag gtcctgctgc agggtccgcc catgcgccag caggtcagcg aaggcctcca
    16381 gtccccccgg cctgacgtag accacattgc cgcgccggcg tacgagcggc tggtccgcga
    16441 acccggccgg cggcgcttcg aggaaggggg cgctggcggc ggtcagaacc cgggtcagac
    16501 cctcggcatg gaccagcgac cattccgcgg cggatcgacc ggtggcgtga aagccaaggc
    16561 catcctcgcg ctccagcacg acgacgctga atcgcgcggc cagccgccag gccagcgcca
    16621 cgccagcgat gccgcccccg acgatggcca ctctcggagc tcccatgacg gcagcatagc
    16681 gaccccggag ggtcgcggga ccggccgtcc ggtctgcggg atgagcgacg ggcccttcgt
    16741 gacggacgtc cgcattggtt ttggacaccc ggcctaagct atggctggag gagtcacggc
    16801 cacatggaag tctcattcgc gagcgaagcg gcccaactgc gccttggcgc gggcgagacc
    16861 ttccgcggcg agggcgtcct tgcggtgacc aaggcgctgc tgcaatccgg cgtcgcctat
    16921 gtcggtggat accagggcgc gccggtcagc catctgctcg acgtcatggt ccagtcgcgc
    16981 gacctgctcg acgaactggg cgtgcacctc gagacctgcg ccaacgaggc cgccgccggc
    17041 gcgctgctcg gcgcctcgat cgcctacccc ctgcgcggcg cggtcacctg gaagtccgtg
    17101 gtcggcacca acgtcgcctc ggacgccctg tccaatctcg cttcggccgg ggtcacgggg
    17161 ggtgtcctga taatccttgg agaggactac ggcgaaggcg ccagcgtcat ccaggaacgc
    17221 agccatgcct tcgcgctcaa gtcatccgtc tggttgctcg atccgcggcc cgacacggcc
    17281 cggcttgtcg acatggtcga gcacgccttc gccctgtccg aggccagcaa cacgccggtg
    17341 atgatggagt tgcggatccg cgcctgccat cgctacggcg tgttcacgac gaaggacaat
    17401 gtccgtcccg cccacgccgg ggcggcgcga ccgatcgcgc cggcgccgtt cgactacggt
    17461 cgcttgtcac acccgccgtc gaccttcctc caggagaagg acaagctttc ccggcggctg
    17521 cctgccgcgc tggactacat ccgtgaccat cggctgaacg acctgatcga cggcgacata
    17581 gccgatgtcg gcctgatcgt gcagggcggc ctgttcaaca cggtgctccc ggagctcgcg
    17641 cgcctcgggt tcgctgaccg cggtggcgcc agccggttgc ccatactggt gctcaacgtc
    17701 gtccatccga tcgcgcccga ggagatcgcc gggttctgcc gggacaagcg ggccgtgctg
    17761 attttcgagg agggcgctcc cgactacatc gagcagttca tcggcgcgac tctcaggcgg
    17821 gcgggtctgt ccacggccct gcatggcaag gacctgctcc cgcccgccgg cgaatatacg
    17881 gcggaagccg tgcgggcggg actgcgcggc ttcctggcga gggccagcct cgaggcggtc
    17941 gatgatggct gggaggccag cgtggccacc gcgcgggcca aggcgaccga tgcgctgggc
    18001 ggtgttccgg accgaccgcc aagcttctgc gtgggctgcc cggaacgtcc gttcttcacg
    18061 gcgttgaagc tggtccagaa ggacaccgga ccgctgcatg tcgccgccga catcggttgc
    18121 cacgccctgg cgacctttcc gccctttgac tcgggccaga ccattctcgg ctatggcatg
    18181 tcgcttgcct cgtcgcaggc cgtcgcgcct tttcaggccc gacgcccgct ggccgtgatg
    18241 ggcgatggag gcttctggca caatggtctg atcaccggcg tcgccggcgg cgccttcaac
    18301 caggccgacc agattctcgt ggtgctggac aatggctaca gctccgccac cgggatccag
    18361 gacataccga gcagccgcaa gggtctcgcg gcctcgatcg aggcagccgt cagggccgtc
    18421 ggcgtccgct gggtgcgcaa ggcgctgacc tatcgcgtcg ccgacatggt cgcgttgctc
    18481 agggaagcgg tgaccaccgc tgagaccgga ctgaaggtca tcgtcgcgga gggcgaatgc
    18541 atgctcgccc gccagaagcg cgagcggtca gcgttgacgg cgagactggc gcgcggcgag
    18601 cgtgtcgtgc gcatcaggta cggcgtcgat gaggcgatct gcacgggcga ccatgcctgt
    18661 atccgcctgt caggctgtcc ctccctgacg ctccggccgc gatcggatcc cttgcgcaat
    18721 gatccggtcg cccatgtgga caacgactgc gtcggatgcg ggctgtgcgg cgaggtggcc
    18781 catgcggccc agctttgccc ctcgttctat cgggccgagc tggtccgcaa tccgcgtcga
    18841 tgggagcgct ggctgcacag ggtcggggtc tgggcctccg gcgcggcggt cgcggcatga
    18901 gctcaccgga tccgatcacg gtcctgatct gcgccctcgg tggcgaaggc ggcggtctgc
    18961 tcgcccagtg gatcacccgg gcggcgacac tggaggggct ggccgtgtcc gccacctccg
    19021 ttcccggcgt cgcccagcga accggggcca ccagctacta cctcgagatc cagccgggcg
    19081 gcgggggcga agcggtgatg ggtctgcatc cggtgccagg ccggatcgac attctggtcg
    19141 ccagcgaact ggtcgaggcg ggacgggcga tgctgcgagg cttcgtcacg cccgacagga
    19201 ctcgcctgat cgcgtcgacc cacagggtct tcaccatttc tgagcgcgcc gcgcccgatg
    19261 atggacgggt tgacggcgac aggatcctgt ccgcggcccg ggacatggcg cgcgaaaccc
    19321 tgctgttcga cgccgccacg ctggccgggg cccatggctc gcgctccaac gccgtgctgc
    19381 tcggcgcgct cgccgccacc ggcgccttgc cgatcagcgc ggcgtcgttc gaggccgcgc
    19441 tggatgcgaa cggttcgggc ggcgccgcga acctcgcggg cttctcggcc ggtatggccg
    19501 cggccgagaa ccgggatgcg ccgctccgac gacccgacga tccccagcct cccgccaccg
    19561 atctcgagcg cgagcttcac ggccttcccg cgggggcccg cgaaaccgcc gcccatggcg
    19621 tcgcgcgtct catcgactac caggggcggc ggcacgcgaa agcctatctc gaccgggtcg
    19681 gcaggatcgc tcgcctggat gctgatggcg ccctgaccga ggccacggcc cggcagctgg
    19741 cgctgctgat gtcgcaggaa gatgtaatcc gggtcgccca gctgaagatc gatccccgac
    19801 ggctggcgcg cgtgcgccgc gaagccggcg cggcgcccgg cgatccctgt cggatccatg
    19861 agttcttgaa gccaggcctg gcggaagccg tggccctgtt gcctgccgcg gccggacggt
    19921 tcctttccgc caggatcgcc gcctcgccgc gcctttcggc gctggcctgg tccctgaaga
    19981 tcgagacgac ggccgtgtgg ggctatctgc tgcttcgggg actggccgcg ctgcggcccc
    20041 tgcggccgct cagtttcatt catgccgacc gggaagccct ggtcgaccgc tggctcggcc
    20101 tggttgagcg tgccgcgccc ctgtcgacag cgcttgcgcg cgaggtggtc gaggccgcat
    20161 cgctggtcaa ggggtacgct tccacgctgg agcggacccg tggcgcgttc tttcgcgtgc
    20221 ttgacgccgt ggtcgagccc gcggtcgctg gcgacctgcc cgaagcccat gccgctgacg
    20281 cggtgttgca ggcgcgactg gccgcccggc gtgacgacga gggccgggcg ctgtccgccc
    20341 tgatcgcggc cttgccgcgc gtgggcaggg atatagccgc atgaacctcg aggatcggga
    20401 cggtctcatc tggctggacg gcgcgcttat cccctggcgc gaggcgcggg tgcacgtgtt
    20461 gtcccagacc ctgcatcacg gcatgggcgc gttcgaaggc gtgcgcgcct atgacggcga
    20521 gtcgggcgta gcggtcttcc gactgcgcga gcacacacgg cgcctgttca actcctgcaa
    20581 gatcgtcggc ctgaccctgc cctggactcc cgaacagatc gacgaggcct gcctcgccgt
    20641 tattcgagcc aacggctacg gcggctgcta cctgaggccg gtggttttcc tcggtggtga
    20701 aaatccgggc gtttcggcgg ccgggaacag cctccacgcc gccgtcatcg cctggccctg
    20761 ggaggactat ctgggggctg gcgcggccca gcggggcgtt cgcctgaagg tatcgtcctt
    20821 cagccgccac catcacaacg ccgtcctgct caaggcgaag gccaacgggc tgtacatcaa
    20881 ctcgatgctg gccaaccagg aagcccggtc gctcggattc gacgacgccc tgatgctcga
    20941 cgtctccggc ttcgtggcgg aggcctcgac ctccaacata ttcgtggtcc gggacggcga
    21001 gatcgccacc ccgccccgga cgtccgttct ggagggtatc acgcgcgcca gcgtgatcac
    21061 gctgctggcc gacctcggcc atccggtggt cgagcgccat ctcacccgcg acgagctcta
    21121 tatcgcggac gaggcctttc tgaccggcac ggcatcggaa ctgacgcccg tctcgtcagt
    21181 cgacgaccgg ccgctccatg cctgccccgg cccggtcacc ctgcaggtcc aggccgcctt
    21241 ccacgcagct gtgcgcggcc gatcgcggga ccggctcgcc tggctgacgc cggtcggacc
    21301 tggacaatga gcggacggac ccaggtcgtc atcgtcggag ccggcccggc cggtctgttc
    21361 ctcggccacc tgctgcaccg ggccggtatc gagacggtgg tgctcgagca gcgcagccgc
    21421 gactatgtcg agggccgggt tcgcgccggc gtgctcgaac aggtgaccat cgaccttatg
    21481 gcccggctcg gactcgacgc ccgcatgcgc cgcgagggtc tggttcattc gggaaccatg
    21541 atcgcctgcg acggcgatct cttccggatc gacatggagg ccctgaccgg cggctcgacg
    21601 atcatggtct acggccagtc cgaggtgatg aaggaccttg gagacggcgc cgccgcgcga
    21661 gggctgcggg tggtctacga cgccgaggcg gtgaccctgc acgacatcga ctccgaccgc
    21721 ccgacggtca cctggcggca ggccggcgag gaacaacgcc tcgcctgcga cttcatcgcc
    21781 ggctgcgacg gtttccacgg cgtctgtcgc gcgagcatac ccggcgcgga ccaggcgacc
    21841 ttcgagcggg tctatccgtt tggctggctg ggcgtcctgg ccgaagtccc gccctgcgag
    21901 gacgagctga tctacgccaa ccatgagcgc ggcttcgcac tggccagcat gcgatcgccc
    21961 acgcggagcc gttactacgt ccagtgcagt ctcgccgacc gggtcgagga ctggcccgat
    22021 gagcggttct gggacgaggt ctgcctgcgg ctcggccggt cggcggccgc gacggtcacc
    22081 cgggggccgt cgttcgagaa gagcatcgcg ccgctgcgca gcttcgtctg tgaggccatg
    22141 cggcacggcc gtctgttcct cgccggcgac gccgcccaca tcgtgccgcc tacgggggcc
    22201 aagggcctga acctcgcggc ttcggacgtc atctatctcg ccgaggcgct gatcgcccac
    22261 tgccagggcg gcgacgacag ggcgctcgat cgctacgccc cccgcgccct ggccagggtc
    22321 tggaaggccg aacgcttctc ctggtggttc acaggcctga cccaccgctt ccccgacacc
    22381 gatccgttcg atcggcgaat ccagctcgct gagctcgcct acctgcgtgg ctcgcgcgcc
    22441 gcccagacca ccctcgccga aaactacgtc ggccttccgc tgagttgaga cccatgagcg
    22501 gcaagatctt cgccaacgcc gacaaggccc tcaagggctt cctgttcgac ggcatgacgc
    22561 tgatgtccgg cggattcggg ctcgtcggaa ttcccgaaga cctgatcgcg gccgttcgcc
    22621 gatccggcgt ccgcgacctg accatcatct ccaacaactg cggcgcgcag ggcgtgggcc
    22681 tctggaccct tctggaaaac cgccagatcc gcaagatggt cagctcctac atcggcggaa
    22741 accgcctgtt cgcggaacgg ctggaggctg gtgatgtcga gctggagctg gcgccgcaag
    22801 gcacgctcgc cgagcgggtg cgcgcgggcg gcgctggcat cccggccttc tacacgcgca
    22861 cgggggtcgg cacgatcgtc gccgaaggca agccatcgga gattttcgag ggccacacct
    22921 atatccgcga gcgctggctc cgcgcggacc tctccctggt cagagcctgg aaggccgacc
    22981 ccgcgggcaa cctccagttc cgcaagactg cccgcaactt caatccggtc atggcgaccg
    23041 cgggacgggt cacggtcgcc gaggtcgagg agatcgtgcc ggcgggcggt ctcgacccgg
    23101 actccgtgca cacgcccggc atctacgtcg atcgggtcat ccgcagccac gcgcccaaac
    23161 gcgttgagaa cctgacgacc cggccccgcc cggcggagac agcctgatgg cctggacgcg
    23221 ggacgagatg gccgcccgcg cggcccgcga actggtcagc ggccagtatg tgaacctcgg
    23281 catcggcatt ccgacgctcg tcgccaatca catcccgccg gacgtcgagg tgatcctgca
    23341 gagcgagaac gggatgctgg gcatgggccc gtttcccttc gaggacgagg tcgatcccga
    23401 cctggtcaac gccggcaagc agaccgtctc ccaacttccc acctcgagct tcttctcgtc
    23461 ggccgacagc ttcgcgatga tccgtggcgg ccacgtcgac ctcaccattc tcggtgcgat
    23521 ggaggtctcc caggccggcg atatcgccaa ctggagcgtc ccgggcgaga cgctcaaggg
    23581 gatgggcggg gcgatggacc tggtcgccgg cgtgcggcgc gtggtggtca tcatggacca
    23641 caccagtcgc gcgggacgac ccaagatcct gaggcgttgt gcgctgcccc tgaccggagc
    23701 ggcctgcgtc gacctgatcg tgacggacct ggccgtgttt gaacgacggg gaggcgggct
    23761 ggtcctgatc gaactcgccc ccgacgtgtc gctcgacgag gtccgggcga agacggacgc
    23821 cgacttcatc tgcggagccc tttcatgacc ggcccgatct ccttcaggcg tgaggacgcc
    23881 gggcagcctg ccctgctgta tcccccctac cgatcgaccc tcctgcggac cccgcgcgaa
    23941 gcgtcggtcc ggataccgca gaccgtgacc gagaccaccg gcccgaccca gtggacgcgc
    24001 ctgatgggcg cggcgatggc cgacctgacg acgcagcacg ccgccccgcc catcggacag
    24061 aggatcatcg tcgccggtcg ggtgctcgat gatcgccgcc gcccggtccc ccgcacggtg
    24121 atggagatct ggcaggccaa cgcggccggc cgatatatcc acaagaagga ccagtgggac
    24181 gcgccgctcg acccgaactt caccggcgcc gggcgggtca tcaccgatga cgagggccga
    24241 tttcgcttcg tcaccattcg tccgggcgcc tatccctggg gcaatcatcc gaacgcctgg
    24301 cgaccggccc atatccacct ctccctgctg ggcccagcct tcgccacgcg actgacgaca
    24361 cagctgtact ttcccgacga cccgctgatt gcgctggacc cgatcgcggg agcggttccc
    24421 gcgccgttcg tcgatcggct ggtcgcccgg ttcgacctgg atctcacgga gccctcctgg
    24481 gcgctcggct atcgcttcga tgtcgttctg cgggggcatg acgcgacgcc catggaggcc
    24541 tgaccatgcc gacgccccct cagcgccccg acctgttcgg ccagaccccg tggcagacgg
    24601 tcgggccctt ctttcactac gggctgccct ggaagggcgg cgcggatctg gtcggtgcgt
    24661 cggacctcgg cgcgcgcgcc gacctcgtgc ccgaggcgca ctacctgctg gccggaccgg
    24721 cgtcgcgagg cgagatcgcc ggggaaatca tcgagatcgt cggccgcgtc acggacgggg
    24781 ccggtgaccc cctgcccgac gctctggtcg agatctggca ggccgacgcc tcggggcgtt
    24841 acaacaccga cgatccggag gggttcatcg ggttcggccg cgccgccacc gacgacgacg
    24901 gggcgtatcg gttccgcacc ctgaagccgg ggcccgctcc gggacccgac ggccgcgccc
    24961 aggctcccca catcgcattc gggatatccg ggcggggact gctgaaacgg ctcgtcacgc
    25021 gactctactt cgggggtgag gcggacaacg ccgacgaccc cgtgctctcg cttgtccccg
    25081 aaccccgtcg ccagacgctg atggccaccg cggtcgccgc atccgcgcac agctggcgct
    25141 tcgacatcgt cctgcagggc gagggcgaga ccgtgttctt cgacttctag ggagccgcgc
    25201 cgtgacctcg ctgatccgag acctggccgc ttcgacgccg gagatgatcg atgtcttcgg
    25261 cgacgcggcc acggtgcggg cggcccttcg attcgaggcc gccctggccg gcgcctgcgc
    25321 ggcggaagga ctggtctcgc cagaggcggc gcgggccgtc atctgcgcct gttcgaaacc
    25381 accggacctg gagtctctcg cccgcgaggc ggctcacgcc ggcgtcctgg ccatcccgct
    25441 ggtgcgctgg ctgcggacga cggccgacac cgtctcgccg ggggccggtg acgccgtcca
    25501 tctgggcgcg acgagccagg acctcgctga caccgcgatg gtgctgcagg cgcgggaggc
    25561 ggcgaggctg gtcgacgggg atctggcgcg cctcgcggac agtctcgcgg atctcgccga
    25621 gacccacgca gcaacgccga tgatcggccg gacgctgctt cagcaggccc tgcccgtcac
    25681 cttcggcctg aaggcggcgc actggatgat gtcgatcgaa ggcgtcaggg cccggctccg
    25741 ccgcgaggcc gacagcgcca tgatgctgca gttcggcggc ccggcgggaa cgctgggggg
    25801 gcttgccggc aagggcgatg cggttgcgcg acggatggcg acggaactgg gcctggggtg
    25861 tccgttgctc ccctggcatt cgcggcgcga agccatcgcc ggcctcgcga gcgcgctggc
    25921 gatcgtggtc ggcgcgatcg ccaagatcgc tctcgatgtc gccctcctcg cccaggtcga
    25981 ggtcggcgag gtctcgattc cgcggcaggg cgatcggggc ggatcctcgg cgatgccgca
    26041 caagcgcaat cccaccggca gccagatcgc cctgtcggcg gcccaccgcg cgccgggtct
    26101 ggcgacgtcg atcctcgcgg gcatgccgca ggctcacgaa aggggcctgg gcggatggca
    26161 ggcggagacg ccagtcctcg tcgagctgtt cctgatcacc cacggggccg tcgtcgccgt
    26221 cgcgggccag gtcgacgggc tggacgtcca caccgacgcc atgcgcagga cgctcgacgc
    26281 cgccggcctg ggtcaggaca tcggcgagtc tctcgacatg gttcgccggg cgctcgccaa
    26341 ccgggagatc cgctgatgcc cttcgccaat catgccggcg cgcgactgta ctggcggcag
    26401 gaaggcagtc ccgacaagcc cgccctgctg ctgctgagct cgatcggtac ggatcagagc
    26461 ctctgggacc tcgccgcgcc tcatctgctg gacgacttcc ggatcctgcg catggacacc
    26521 cgcgggcacg gcgcatcgga cgcgccaacc ggcccctaca gccttgccca gctggcgggc
    26581 gacgccctgt ccgtgctgga cgccgccggc gccgaccagt cgacgatctg tggagtctca
    26641 cttggaggga tgatcgccct gcgtctggcg ctggacgcgc cggagcgtgt ggccggcctg
    26701 gcgatcgcct gcacgtccgc ggagatggac cgggcggcct ggcaggcccg gatcgacacg
    26761 gtgaccgcag aagggaccgg cgccatagcc gacatggcca tgcaacggtt cttcccggag
    26821 gcctttcgac ggtcccaccc ccgggtggtc gataccgtcc ggggcggcct gctggcgacg
    26881 gacccgacgg gctacgccgg ctgctgcgcc gccatccgcg acatggatat cctgtcccgg
    26941 cttggcgcca tcgccgcccc gaccctcgtc atccacggtc tgcgggacat ctccacgcca
    27001 ttcgtgggcc acggagatcg gatcgcggcg gccatcccgg gggccaggtc ggccgccctc
    27061 gacaccggtc acctgccctg cctcgaggcg ccgggcggtt tcgccgcact ggtacgccgt
    27121 ttccaccagc ggacggtcca cgggaacgtc gaggacgacg ccgcggacgc tctctacgcg
    27181 gcagggctcc gcacgcgtcg gaaggtgctc ggtgacgcct gggtcgacgc ctcccttgag
    27241 tccaggaccg acttcaacgc cgatttccag gcgatgatca cccggtgcgc ctggcgcgag
    27301 gtctggagcc gcccgggtct ggaccaccgg acccggcgcc tgctggtcgt ggcgatgacg
    27361 gcaagtctcg gtcgctggga ggagttcagg ctccacgtcc gatccggcct ggatgccggc
    27421 ggcttcaccg tcgacgagtt gaaggaggtg ctgatgcagg tcgccatcta tgcaggcgtg
    27481 ccggcggcga acaccgcctt cgccgaagcc ggcgggatcg tcgaaagctg gcgtgcgcgc
    27541 ttcggcgcct gacgccagcg cccgatgacg gacaacccga acgccgtgcg accggacgct
    27601 ggcgcggcgg cgcgaagcat tgtctggctg ggcgtgcgga cctgaagggg cagagccatg
    27661 aaaatcgcct tcatcggcct tggggtcatg ggcttcccga tggcgggcta tctggcgaag
    27721 gccgggcatg aggtcagcgt gttcaaccgc ggcccggcca aggc
//
LOCUS       DAORNX010000003        22221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_1810_length_22221_cov_8.241406, whole genome shotgun sequence.
ACCESSION   DAORNX010000003 DAORNX010000000
VERSION     DAORNX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 22221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22221
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1810_length_22221_cov_8.241406"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLO65_00280"
     CDS             complement(<1..880)
                     /locus_tag="PLO65_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD26714.1"
                     /translation="MKTATTGVWGVIAALAVALTLAGCGRPEGNEKPPQPGDTAVARI
                     DGKTVWASDVKREAVAQGLISEGEPLDTSSDLFRRVMDEVVDQKLLAAEALKRKLDRD
                     PVAQRRLAAARERILGDMLVESVVEKAVTPGAIRDLYQEQLRLSKQSEELKARQLVVA
                     TQAEAEQIKKLLAAGASFEALAMERSTDAATRFSGGDIGGYFTLDVMPEPYVAALKDA
                     KPGDLVGPFAVEGGWALVKVEEKRQEAPITFEAARPQIVRFLTYDKVRDLLEKLRAAA
                     KVQVLLGKPQDVPGAPR"
     gene            1263..4034
                     /gene="secA"
                     /locus_tag="PLO65_00285"
     CDS             1263..4034
                     /gene="secA"
                     /locus_tag="PLO65_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRD26715.1"
                     /translation="MADFFKTLFGSSNDRKVKQLMGRVAKINALEPEYAALTEAQLRG
                     KTDEFKARLAAGETLDNLLSEAFAVVREASKRTLGQRHYDVQMVGGMVLHKGGIAEMR
                     TGEGKTLVATLPTYLNALEGKGVHVITVNDYLAKRDADWMGQIYRYLGLSVGVIVNGL
                     SQMQRQEAYGSDITYGTNNEFGFDYLRDNLVYDVNEMVQRGHNFVIVDEVDSILIDEA
                     RTPLIISGPTEDRSEFYRTMDVVVKALIKEEGVFEHDEKQRQVLLTEAGSERVEEILQ
                     ANGQLAPDSAGLYDPVNISVVHHINQALRANVLYTKDKDYIVKAGEVILIDEFTGRMM
                     TGRRLSEGLHQAIEAKEGAEIQPENQTLASVTIQNYFRLYKKLSGMTGTAATEAQEFL
                     DIYKMDVTEIPTNRPVARIDEDDEVYRTADEKNRAILAQIEDCHRRGQPILVGTVSIE
                     KSEQLSELLSKHEFEIDGKTVKGIPHNVLNARYHEQEAYIVADAGAPGAVTIATNMAG
                     RGTDIQLGGNVEMHMAKWRQDQMGLGLAVDHDAEMAEKHRFEAEITEAKQKALAAGGL
                     YVLGTERHESRRIDNQLRGRTGRQGDPGKSKFFLCCEDDLLRIFAGERLDAIMRSFGV
                     QEGEAITHKWLNKAIATAQKRVEQRNYEIRKNLLKYDDVVNDQRKAVFEQRQEFMEAT
                     DLSEIVAQMRQDVIDDMVARDLPPKAYAEQWDIDDLDERVRTTLGLELPLREWAAEEG
                     IANEEIRDRLDAAANARATEREELVSAGQMRGFEKNFLLQMIDMQWREHLVHLDHLRN
                     VIGLRGYGQRDPLNEYKTEAFSLFEKLLVDLRTNVTRWLMTVEFQFAEPEPELQPLQF
                     QEVHMDPLTGENERALAFAGAGADMSGEQRAALPISALPAGWERTSRNADCPCGSGRK
                     FKHCHGRLI"
     gene            complement(4125..5516)
                     /locus_tag="PLO65_00290"
     CDS             complement(4125..5516)
                     /locus_tag="PLO65_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRD26716.1"
                     /translation="MDRDVRVNPYLAGAFAPLRSEDDFELVIEGEIPAGLRGALYRNG
                     PNPQFEPRDPNHHWFAGDGMIHGFWVEDGKVRYRNRYVRTPKWEAENKAGKSLFGTFG
                     NPMTTDPEVFGKVDSGVANTNIVWHGGRLLALEEGHQPFGLDPKTLDSIGYQDFGGKV
                     TAHPKEDPVTKELVFFGYADAPMPFSPNVSYGVMGADGVLKRRDTFEAPYCSMIHDFM
                     VTAGHVLFPILPLSGSMERAMKGLPPFAWEPEKPALVGVMARDAAVDSIRWFQTDACY
                     VFHVMNAWDEDGRIVADVMKYAVAPLFPLADGSRGTQAGAYLVRWTFDLNDASNVIRE
                     EPLDDLAGEFPRYDERFTFKANRHGWYSTQTAKPGDIRTNGLSHIDLTTRKRTDWVSG
                     AGDAVSEPVFVPRSADAPEGDGWIVAVQYLASEARSDFLVFEAQSLDKGPIGRAKLPR
                     RVPLGFHGNWVGA"
     gene            5628..6704
                     /locus_tag="PLO65_00295"
     CDS             5628..6704
                     /locus_tag="PLO65_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRD26717.1"
                     /translation="MTAELGIIEGYYGKPWSWAERAEQVTFLAPHGYRFFMYAPKADA
                     FLRKRWREAHPATEAAALAAVSAHCRSLGVRFGVGLSPFELYRDFGQAAKADLARKLA
                     ELDAIGIDDLGILFDDMRGDLPGLAQSQADILHWIAERTRAGRVIVCPTYYSDDPVLD
                     RFFGERPANYLEDLGAALDPAIGVFWTGEEVCSAEYSPGHLARVAGQLGRRPILWDNY
                     PVNDGPRMSNFLHLRAMTGRPAAIGRHLSGHAVNPALQPVLSRIPLLSLADSYREGEA
                     YQYGAATARAAAAVLGDELGATVHGRLLVLQLTGLDRMEPETVASLRARFAAFDHPGA
                     REIVRFLDGDYRITREMMAEEGTG"
     gene            complement(6866..7930)
                     /locus_tag="PLO65_00300"
     CDS             complement(6866..7930)
                     /locus_tag="PLO65_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HRD26718.1"
                     /translation="MAAQGWDFLVRRNDLHETRIAPVALTAPAAGEVLFRVESFALTA
                     NNVTYAVAGDQIGYWRFFPKADGWGCIPTWGYGVVEASGHPDFAPGERFYGYWPMGSH
                     LLATPRRSKAGFVDEAAHRADLPPTYNQYVAAPAEATLQDQRSLLNPLFMTSFLIDDL
                     LVDNDDFGARTVILASASSRTALGLAWLLKRSGRVKVVGLTSPRNRAFVEGLGYYDQV
                     VVYDDLGAAPVETPAVFVDFAGDPAVLAAAHNRFGDDLAYSCLVGLTHWEARGAPADL
                     PGPRPVFFFAPDRVVKRRQDWGPGGLEARYGEAWQGFVADTPRWLTLKTFTGAEGVKT
                     AFLDVLDGKAPSSEGLICRP"
     gene            7999..8601
                     /locus_tag="PLO65_00305"
     CDS             7999..8601
                     /locus_tag="PLO65_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD26719.1"
                     /translation="MPRLLTDSDVADFRERLIAAAERLFGERGPDGVTMRQLAAELGV
                     SPMTPYRYFKDKDAILAAVRASGFARFAEAMEQATAGITDPQQQSEATGRAYVRFALG
                     NPDAYRLMFDFSQPNEADYPELLEASARARTMMARHAQALVASGEARGDPMMIAHILW
                     AALHGGLVLQMAGKLSPDIDPSALRRQAFETILRGLKPDA"
     gene            8750..9916
                     /locus_tag="PLO65_00310"
     CDS             8750..9916
                     /locus_tag="PLO65_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HRD26720.1"
                     /translation="MTQPKPFTVDLSEFEVRRVLDQVRHYPWPPAPAVDDGWLYGCDA
                     TFLEETCAYWADHYDWRAAVAELNRFPQFTARVEEFDIHFVHVVGEAGGKRPLLLTHG
                     WPGSHYEFWASIGKLAFPSRHGGKAEDAFDVVVPSLPGFGFSSKPARPVGQRLTAKLF
                     NTLMTEVLGYDQYLAQGGDWGGLVTSWIGFDHGAPSEGKKGAKAIHLNMIGFRPKGGP
                     QSPEEVAWITRQQAMMGMLGAYFGLQASKPQSLAWLGAGNPVGQAAWILERFHDWSDL
                     RSKPLIGAYTRDQLITNVMIYVMTGSFTTGAWYYRGLMEEGGIAAMEGQRCETPTAFA
                     NFPGEPLYTAPPRSWADRAYNITRWNDMPSGGHFAAMEEPDLFVADVRDWAASL"
     gene            complement(10028..11308)
                     /locus_tag="PLO65_00315"
     CDS             complement(10028..11308)
                     /locus_tag="PLO65_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26721.1"
                     /translation="MTTTVQAGAAPSNRTVLTASLVGTAVEFYDFYIYATAASLVFGP
                     LFFPKDSASAQLMLSYATFAIAFIARPVGAVFFGHFGDRIGRKSTLVASLLLMGGSTT
                     AIAFLPGYAMAGWIAPLLLCLLRFGQGFGLGGEWGGAALLAVENAPPGWRARFGMFPQ
                     LGAPVGFIAANGLFLILGLVLSPEQFLSWGWRIPFLLSAVLVAIGLWVRLSLTETPAF
                     KAALEEAPPPAVPLGEVMRTNWREVLGGTFGVVACFCIYYLSTAFALGYGVKTLGYSR
                     EAFLGVQLIAILFMAGGIIWAGSWSDASNPRRVLMVGCVLAVLAGFGLAPMLGSGSLL
                     LVGAFLSVAMVLMGLVYGPLGAWLPGLFPARVRYTGASVAFNVGGIIGGGMTPLIAEG
                     LAKAGGLTYVGFYLSGACALSLAALLLLKPKPKS"
     gene            11518..11688
                     /locus_tag="PLO65_00320"
     CDS             11518..11688
                     /locus_tag="PLO65_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26722.1"
                     /translation="MTDTPLTPAEKMKAILAAKQGGLARAGQGNAASRGRHGESIAAA
                     RSKSQSKPALRK"
     gene            11694..12701
                     /locus_tag="PLO65_00325"
     CDS             11694..12701
                     /locus_tag="PLO65_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRD26723.1"
                     /translation="MARYDFASDNVAGAMPEVMDALIAANAGTASGYGTDHISRMAAD
                     LIRAALDADAEVRFVASGTAANGLSCAVLAQSHEAVLAHEHAHILTDETGAPGFFGAG
                     VGLIGLPGASGRIDPAALAAALAQPDLSYRQSAAALSLTNTTEYGTVCSVESFKALID
                     PVKARGYGVHLDGARLANAVAAGFDLKAVPKLGVDIVIMGGTKSGSTPTEAIVLLNKA
                     LTRRFDARLKHAGQLVSKGRFLAAPWIGLLETDAWRTRAAHANAMARRLAALMPFPIV
                     HPVEANGVFVEMDQPTLDRLAATGWFVYRFIDDTVRFMCSWATTPEMVDELGEALASI
                     K"
     gene            12888..14042
                     /locus_tag="PLO65_00330"
     CDS             12888..14042
                     /locus_tag="PLO65_00330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD26724.1"
                     /translation="MSEPSHPEIREAVRKLCAGFPGEYWRALDRERTYPTEFVRTLTE
                     AGFLSVLIPEEYGGSGLGLSAATAVLEEIHRSGCNGGACHAQMYTMGTVLKHGSPAQK
                     EAYLPRIASGEIRLQAFGVTEPTAGTDTTRISTFARREGDKYIVNGQKIWISRAEHSD
                     LMVLLVRTTAREDAARPADGMSVLLVDMREAVGRGLTIRPIRTMLNHATTELFFDNLE
                     VPAGNLVGEEGKGFRYILDGMNAERILIAAECIGDGRFFIDRSSAYAKERNVFGRAIG
                     ENQGVQFPIARNYVQLSAAALAVDHAAALFDAGKPCGTEANMAKMLASEASWAAADTC
                     IQTHGGFGFAEEYDIERKFRETRLYQVAPISTNLILSHVGTHVLGMPKSF"
     gene            14054..14860
                     /locus_tag="PLO65_00335"
     CDS             14054..14860
                     /locus_tag="PLO65_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD26725.1"
                     /translation="MPTFIPIDDPADPRLEAYRDIRERDLVGRQGRFIAEGETVLRVL
                     AGQDRFRPLSVLIAPKRLEKLAPILGQLADDAPVYLAGQSVLDAIVGFELHRGILALA
                     ERAAPVAADDLLAGLPGKALVLALVGISNHDNMGGLFRNAAGFGVDAALLDPTCCDPL
                     YRKAIRVSVGGVLRVPYARLGEGEDLVDLLEQRGFTPVALSPSGDTALSDLPALSRPA
                     LLLGAEGPGLPAATLARCRTVSIPMAPGFDSLNVAVAAGIALHQLRSARS"
     gene            complement(14857..16509)
                     /locus_tag="PLO65_00340"
     CDS             complement(14857..16509)
                     /locus_tag="PLO65_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD26726.1"
                     /translation="MNRKLAWTLGSIVVVLVAGFIAFQHFWIYIPGVIGRIKNPIQPY
                     HQVTWASGAAPALAPGAKRPPNVIVIVADDLGINDLQSTGQGVANGAVPTPNIDSLAR
                     DGVAFTNGYAGNATCSPSRAAMMTGRYPIRFGFEFTGVPVAFAKNIARMGGHDGVRPV
                     FYADRVRDMPPVEKLGVPASEVTLAETMKAAGYRTLHIGKWHLGEADGMRPENQGFDD
                     TLNILAGGGMYMEANDPNVVNSKQDFDPIDRFLWANLPWSVRFDGSKPFHPNQYITDY
                     FGDEAVRAIDANANRPFFMYLAFNAPHTPLQALKSDYDALPQIKDHRLRVYAAMIRAL
                     DRNVGKVLAELKAKGLEGDTIVIFTSDNGGANYIGLPDINKPYRGFKATFFEGGVRVP
                     FFVRWPGQIQPGTTTPARAMHFDIFATAAAAAHAPPAGPGVLDGVDLVALSRGQVSDR
                     PLFFRSGHYRVVINGDWKLQLSERPKKAWLFNLKSDPTEQSNVATANPAIVAQLTAML
                     DAQDRRAAKPIWPALIEGPIWIDQPADGTVKPKDAEYVMWAN"
     gene            complement(16524..17594)
                     /locus_tag="PLO65_00345"
     CDS             complement(16524..17594)
                     /locus_tag="PLO65_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD26727.1"
                     /translation="MKRVFTGLLLVLVLLAAGAWAVRGKIAVRLMERRLETAMAHPVV
                     PSLPDGLHVAICGAGGPFPDGDRGGPCTAVIAGKRVFIVDTGEAAGRTLGRMGIGGDS
                     VVLLTHFHSDHIDGLGNVALQRWGGGNLTTPLQLYGPPGVERVAAGFTEAYALDAGYR
                     TAHHGPVIMPPSGAGVQARPFAIPEGQDSVVVLETDGVKITAFRVDHGPVEPAVGYRF
                     DYKGRSVVISGDTAPSPVVAKYARGTDLLIHEALSTPLVTLIRDASLKAGNTKRAQIM
                     LDIQNYHTSPSAAAAIARDDGVGALLLNHIVPPLPLRALEGPFLGDARKVFTGPLWIA
                     RDGDLISLPVGGKTITRSSLLH"
     gene            17700..18602
                     /locus_tag="PLO65_00350"
     CDS             17700..18602
                     /locus_tag="PLO65_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HRD26728.1"
                     /translation="MQVLRTPDDRFANLPDFPYSPHYETIRDPSGVDLRIGYIDEGPR
                     DAAPVLLMHGEPSWSYLYRGIIAPLKDRHRVVAPDLVGFGRSDKPAEKTDYSYEKHVA
                     WMSAWLLALDLNDITLFCQDWGGLIGLRLVAAFPERFARVIVANTGMPTGTGMSEGFK
                     TWLTMSQTIPVFPAGMIVNMGSLRELSPDEIAAYDAPYPDESYKSGARIFPTFVPVTP
                     DHPSVAENKAAWTVLERFDKPFLTAFSDGDPVSKGGDRPFQERVPGARGQPHTTIEGG
                     GHFLQEDKPAEIAALIDRFISETR"
     gene            18602..19015
                     /locus_tag="PLO65_00355"
     CDS             18602..19015
                     /locus_tag="PLO65_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD26729.1"
                     /translation="MNVENAVTPTGDQLKALLALGGDRPIVMVNLLKFREKAQYDEPG
                     EPEMTGREAYDRYGRVMGPLVASKGGRLRFSSRVDALVIGEVGELWDAVAIMEYPSRA
                     AFVAIATSPEVQEIGRHRKAGLAGQLLIQCSEAGV"
     gene            19015..19431
                     /locus_tag="PLO65_00360"
     CDS             19015..19431
                     /locus_tag="PLO65_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26730.1"
                     /translation="MPAEARAPSRITLGLLVLLGLINLVRGAIHVFLPDSGAGVIAHF
                     ELAEGGRTIVFLLAMVGAGQIGSGLIDLLVVARFRAFAAPLLAIELLRALIALYIAFV
                     SKSVGTGYPGARGIVGAAVVLTLAVLWEFGRRGRRG"
     gene            19471..20079
                     /locus_tag="PLO65_00365"
     CDS             19471..20079
                     /locus_tag="PLO65_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD26731.1"
                     /translation="MTAPPKPPLAADRVIDGRRLRSEASRLRIVEAMIALVSEGLAMP
                     PAEAVAARAGVGLRTVFRLFEDMDGLYRGIQAVVTERLGGLLEGPIEAADWRQAVDIL
                     IDRRAEAFEMILPLQIAADSPRTRSAALREGRLRLVQAQRDTLLAVLPASVQADGRLV
                     EALDLAVSFEAWRRLRTDQHLDSDAARAVMHRLAQALLHGLA"
     gene            20076..21008
                     /locus_tag="PLO65_00370"
     CDS             20076..21008
                     /locus_tag="PLO65_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRD26732.1"
                     /translation="MKQLVLLLALGLAGCGAKAPQLAAPTRACPGPAPLGEVALKGGT
                     FTLGANPEHIDEGPPRTVTVAPFSIDRTEVTNAEFAAFVKATGYVTLAERQPDPALYP
                     GVPAGKLKPSSLVFVGADALNDGNPMQWWRIIDGASWKHPEGPGSDLKGRERHPVVQV
                     AYDDALAYARWKGRDLPTEAEWEFAARAGLDGARYEWGDDKPVPGRPRANSWQGVFPA
                     VDIGDDGYKARTAPVGCYPASAYGLYDMTGNVWEWTKDWYETPNPNGRSHLIKGGSFL
                     CADNFCYRYRPSARQPGPPDTGTSHVGFRTVKRG"
     gene            21207..22043
                     /locus_tag="PLO65_00375"
     CDS             21207..22043
                     /locus_tag="PLO65_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD26733.1"
                     /translation="MGGITVQKVAGALGAEIGGVDLSAPLDDETIAAIRRALVEHQVT
                     FFRDQSLTPEQQLAFGRRFGPLNIHPYVAGMADHPEVMEIIKEPTDRINFGGGWHSDM
                     SFLETPAIGSILYAVELPDWGGDTLFASQAAAFEALSPGLQATLEGLNAIHSAEREYA
                     PTGPSAQKRESMKISDAEGMAGRFSHPVVKVHPETGRKALYVNPAFTMQIDGWSRRES
                     RPLLNYLFEHSRQEAFTCRLRWRPGTVAFWDNRSVWHYALNDYPGQRRHMRRVTVDPV
                     AA"
BASE COUNT         3549 a         7638 c         7674 g         3360 t
ORIGIN      
        1 cgcgcggcgc gccgggcacg tcctgcggct tgcccagcag cacttgcacc ttggccgcgg
       61 cgcgcagttt ctccagcaga tcgcgaacct tgtcgtaggt caggaaccgg acgatctgcg
      121 gccgggcggc ctcgaaggtg atcggcgcct cctggcgttt ttcctcgacc ttgaccaggg
      181 cccagccgcc ctcgacggcg aacgggccga ccaggtcgcc cggcttggcg tccttgagcg
      241 ccgccacata gggctcgggc atcacgtcga gcgtgaagta accgccgatg tcgccgccgc
      301 tgaagcgggt cgcggcgtcg gtcgatcgct ccatggccag ggcctcgaac gaggcgccgg
      361 cggccagcaa cttcttgatc tgctcggcct cggcctgggt cgcgacgacc agctggcggg
      421 ccttcagctc ctccgactgc ttcgacagcc gcagctgctc ctggtacagg tcgcggatcg
      481 cgccgggcgt cacggccttc tccaccacgc tctcgaccag catgtcgccg aggattcgct
      541 cgcgcgcggc ggccagccgt cgctgggcga ccggatcgcg gtcgagcttg cgcttgagcg
      601 cctcggcggc gagcagcttc tggtcgacga cctcgtccat cacccgccgg aacaggtccg
      661 acgaggtgtc gagcggctcg ccctcgctga tcaggccctg ggcgaccgcc tcgcgcttga
      721 cgtcggacgc ccagaccgtc ttgccgtcga tccgcgcgac ggccgtgtcg cccggctgcg
      781 gcggcttctc gttgccctcc ggccgaccgc agccggccag ggtcaaagcc acggccaggg
      841 cggcgatgac gccccaaacg ccggtcgttg cggtcttcat ggcggcctgt tctccacggg
      901 cggctggccg tccggtccta gcgcggtttc gggcgaaggg aaatgcggct cgccggacag
      961 gcgggactcg gatgcagcgg ggatgcggtg gggacaggtt aagctgtccc tcgggactgc
     1021 ttaacctgtc ccccgatgtc ttcccgatgt cttccggcca gttgcgcccg gacccttgat
     1081 cgggacctgt ggcgcaaagg cttcacgcaa cggcgccctc gccacccctc gcgttgacac
     1141 tggcagaccc gctgcttaag tctcccgcgc gcttgaacgg gcggatttcc gtgcgcccga
     1201 aagggcttgc acgaacaccg aacggccgcc gcgcctgaac atttcaccgg atttcccgac
     1261 gcatggccga cttcttcaag acccttttcg gatcttccaa cgaccgcaag gtcaagcagc
     1321 tcatggggcg ggtcgcgaag atcaacgccc tggagccgga atacgcggcc ctgaccgagg
     1381 cccagctgcg cggcaagacc gacgagttca aggcgcggct ggcggccggc gagacgctcg
     1441 acaacctgct ctccgaggcc ttcgccgtgg tgcgcgaggc gtccaagcgg accctcggcc
     1501 agcgccatta cgacgtgcag atggtcggcg gcatggtgct gcacaaaggc ggcatcgccg
     1561 agatgcgcac cggcgaggga aagacgctgg tggccaccct gcccacctac ctcaacgcgc
     1621 tcgagggcaa gggcgtccac gtcatcaccg tcaacgacta cctggccaag cgcgacgccg
     1681 actggatggg ccagatctac cgctacctcg gcctcagcgt cggcgtgatc gtcaacggcc
     1741 tgagccagat gcagcggcag gaggcctacg gcagcgacat cacctacggc accaacaacg
     1801 agttcggctt cgactacctg cgcgacaacc tcgtctatga cgtgaacgag atggtccagc
     1861 gcggccataa cttcgtgatc gtcgacgagg tcgactcgat cctgatcgac gaggcgcgca
     1921 cgccgctgat catctcgggc ccgaccgagg accgctccga gttctatcgc acgatggacg
     1981 tggtggttaa ggcgctgatc aaggaagagg gcgtcttcga gcacgacgag aagcagcgcc
     2041 aggtgctgct gaccgaagcc gggtccgagc gggtcgagga gatcctgcag gccaacggcc
     2101 agctggcccc cgactcggcc ggtctctacg acccggtcaa tatctcggtg gtgcaccaca
     2161 tcaaccaggc cctgcgggcc aacgtgctct acaccaagga caaggactac atcgtcaagg
     2221 ccggcgaggt gatcctgatt gacgagttca ccggccgcat gatgaccggc cgccgcctgt
     2281 ccgagggcct gcaccaggcc atcgaggcca aggaaggcgc cgagatccag cccgagaacc
     2341 agaccctggc ctcggtgacg atccagaact acttccgcct ctacaagaag ctgtcgggca
     2401 tgaccggcac ggccgcgacc gaggcccagg agttcctcga catctacaag atggatgtca
     2461 ccgaaatccc gaccaaccgt ccggtcgcgc ggatcgacga ggacgacgaa gtctatcgca
     2521 ccgccgacga gaagaaccgg gcgatcctcg cccagatcga ggactgtcac cggcgcggtc
     2581 agccgatcct ggtcggcacg gtgtcgatcg agaagtccga gcagctgtcc gaactgttgt
     2641 cgaagcacga gttcgaaatc gacggcaaga cggtcaaggg catcccgcac aacgtgctga
     2701 acgcccgcta ccacgagcag gaagcctata tcgtcgccga cgccggggcg ccgggggcgg
     2761 tgaccatcgc caccaacatg gccggccgcg gcaccgacat tcagctcggc ggcaacgtcg
     2821 agatgcacat ggccaagtgg cgtcaggacc agatggggct gggcctggcc gtcgaccacg
     2881 acgccgagat ggccgaaaag catcgcttcg aggccgagat caccgaggcc aagcagaagg
     2941 cgctggccgc cggcggcctc tatgtgctgg gcaccgagcg ccacgaaagc cgccgcatcg
     3001 acaaccagct gcgcggccga acgggccgcc agggcgaccc cggcaagtcg aagttcttcc
     3061 tctgctgcga ggacgacctg ctgcggatct tcgccggcga gcgactcgac gccatcatgc
     3121 gctcgttcgg ggtgcaggaa ggcgaggcga tcacccacaa gtggctcaac aaggccatcg
     3181 ccaccgccca gaagcgggtc gagcagcgca actacgagat ccgcaagaac ctgctgaagt
     3241 acgacgacgt cgtgaatgac cagcgcaagg ccgtgttcga gcagcgccag gaattcatgg
     3301 aggcgaccga cctgtcggag atcgtcgccc agatgcgcca ggacgtgatc gacgacatgg
     3361 tcgcccgcga cctgccgccc aaggcctacg ccgagcagtg ggacatcgac gacctcgacg
     3421 agcgcgtgcg cacgaccctc ggcctcgagc tgccgctgcg cgagtgggcg gccgaggagg
     3481 gcatcgccaa cgaggagatc cgcgaccgcc tcgatgcggc cgccaacgcc cgcgccaccg
     3541 agcgggagga actcgtttcc gccggccaga tgcgcggctt cgagaagaac ttcctgctgc
     3601 agatgatcga catgcagtgg cgcgagcacc tggtccacct ggaccacctg cgcaacgtca
     3661 tcggcctgcg cggctacggc cagcgcgacc cgctgaacga gtacaagacc gaggccttca
     3721 gcctgttcga gaagctgctg gtcgacctgc gcaccaacgt cacccgctgg ctgatgacgg
     3781 tcgagttcca gttcgccgaa ccggagccgg agctccagcc gctgcagttc caggaagtcc
     3841 acatggaccc cctgaccggc gagaacgagc gggccctggc gttcgccggc gccggggcgg
     3901 acatgtcggg tgagcagcgc gcggccctgc cgatcagcgc cctgccggcc ggctgggaac
     3961 gcaccagccg caatgctgac tgcccctgcg gctcgggccg caagttcaag cactgccacg
     4021 gccggctgat ctaggaaccg gggacatgca cttcagtgcg tgtccctagc agcttcgaag
     4081 cggcgaggga cacgcactga agtgcatgtc cctgcgctgc gccgctacgc cccgacccag
     4141 ttgccgtgga agcccagcgg cacgcgacgg ggcagcttcg cgcgcccgat cgggcccttg
     4201 tcgagcgatt gggcctcgaa caccaggaag tcgctccgcg cctcgctcgc caggtactgc
     4261 accgccacga tccagccgtc gccctcgggc gcgtcggccg agcgcggcac gaacaccggt
     4321 tcgctgacgg cgtcgccggc gccgctgacc cagtcggtgc gcttgcgggt ggtcaggtcg
     4381 atgtggctca ggccgttggt gcggatgtcg ccgggcttgg ccgtctgggt cgaataccag
     4441 ccgtggcggt tggccttgaa ggtgaaccgc tcgtcgtagc gcgggaactc gccggccagg
     4501 tcgtcgagcg gctcctccct gatcacgttc gaggcgtcgt tcaggtcgaa ggtccagcgc
     4561 acgaggtagg cgccggcctg ggttccgcgg ctgccgtcgg ccagcgggaa cagcggcgcg
     4621 accgcgtatt tcatcacgtc ggcgacgatc ctgccgtcct cgtcccaggc gttcatgacg
     4681 tggaacacgt agcaggcgtc ggtctggaac cagcggatgc tgtcgaccgc cgcgtcgcgg
     4741 gccatgacgc cgaccagggc cggcttctcc ggctcccagg cgaacggcgg caggcccttc
     4801 atcgcccgct ccatgctgcc gctcagcggc aggatgggga acagcacatg gccggcggtg
     4861 accatgaagt cgtggatcat cgagcagtag ggcgcctcaa aggtgtcgcg ccgcttgagc
     4921 acgccgtcgg cgcccatcac gccgtagctg acgttgggcg agaagggcat cggcgcgtcg
     4981 gcgtaaccga agaacaccag ctccttcgtc acggggtctt ccttggggtg ggccgtgacc
     5041 ttgccgccga aatcctggta gccgatcgag tcgagggtct tcgggtcgag gccgaagggc
     5101 tggtgcccct cttcaagcgc cagcagtcgg ccgccgtgcc agacaatgtt ggtgttggcc
     5161 acgcccgaat cgaccttgcc gaacacctcc gggtcggtgg tcatggggtt gccgaaggtg
     5221 ccgaacagcg acttgcccgc cttgttctcg gcctcccatt tcggggtccg gacgtagcgg
     5281 ttgcggtagc ggaccttgcc gtcctcgacc cagaatccgt ggatcatgcc gtcgccggcg
     5341 aaccagtggt ggttgggatc gcgcggctcg aactgcgggt tggggccgtt gcggtagagg
     5401 gcgccgcgca gaccggcggg aatctcgccc tcgatgacca gctcgaagtc gtcttccgag
     5461 cgcagcgggg cgaaggcccc ggccagatag gggttcacgc gcacgtcacg gtccatgtcg
     5521 gcctcccggc ggcattagtt atttacgtcg taaatttatg cgaccgatat gggggcttgg
     5581 caagcgctgt ttgtcggggc gggatggtcg ggcggaggac cgagggcgtg acggcagaac
     5641 tgggcatcat cgagggctac tacggcaagc cctggtcgtg ggcggaacgg gcggagcagg
     5701 tcaccttcct cgcgccacac ggctaccgct tcttcatgta cgcgcccaag gccgacgcct
     5761 tcctgcgcaa gcgctggcgg gaggctcatc cggcaaccga agccgccgcg ctggcggccg
     5821 tctcggccca ttgccgttcc ctcggcgtgc ggttcggcgt gggtctgagc cctttcgagc
     5881 tgtatcggga tttcggccag gcggcgaagg ccgacctggc ccgcaagctg gccgagctcg
     5941 acgccatcgg aatcgacgac ctgggcatcc tgttcgacga catgcgaggc gacctgccgg
     6001 ggctggcgca aagccaggcg gacatcctgc actggatcgc cgaacggacc cgggccggcc
     6061 gggtgatcgt ctgccccacg tactacagcg acgatccggt gctcgaccgg ttcttcggcg
     6121 agcgcccggc caactacctc gaggatctgg gcgccgcgct cgacccggcc atcggcgtct
     6181 tctggaccgg cgaggaagtc tgctcggccg agtacagtcc cggtcacctg gcgcgggtcg
     6241 ccggccagct cgggcgcagg ccgatcctgt gggacaacta tccggtcaac gacggcccga
     6301 ggatgtcgaa cttcctgcat ctgcgagcca tgaccggccg tcctgcggcc atcggccggc
     6361 acctgtccgg ccacgccgtg aacccggccc tgcagccggt gctgtcgcgc attcccctgc
     6421 tgtccctcgc cgacagctac cgggagggag aggcctacca gtacggcgcg gccacggcgc
     6481 gcgccgctgc ggccgtgctc ggcgacgaac tgggggcgac ggtccacggc cgcctgctgg
     6541 tgttgcagtt gaccggcctc gaccggatgg agccggagac ggtcgcctcg ctgcgcgccc
     6601 ggttcgccgc cttcgaccat cccggcgcgc gggagatcgt ccggttcctc gacggtgact
     6661 accgcatcac ccgcgagatg atggccgagg aggggacggg gtgagattca ctcaccaccg
     6721 tcatggcccg acttgttcgg gccacccatg cgtggtgagc gaacaaggct ggcgaaactg
     6781 tcccagtcac ccccgcaggg tccatgttcc tgggtggccc gaacaagtcg ggccatgacg
     6841 gagaggaaga tggggacgga tcggcctaag gccggcagat cagcccttcg ctggacggcg
     6901 ctttcccatc cagcacatcg aggaaggccg tcttcacgcc ctccgcgccg gtgaacgtct
     6961 tcaaggtcag ccagcgcggc gtatccgcca cgaacccctg ccaggcttcg ccgtagcggg
     7021 cctccagccc gccggggccc cagtcctgac ggcgcttcac cacccggtcg ggggcgaaga
     7081 agaacaccgg tctgggtccg ggcaggtcgg ccggggcgcc ccgcgcctcc cagtgggtga
     7141 ggccgacgag gcagctgtag gcgaggtcgt ccccgaagcg gttgtgcgcg gccgcgagca
     7201 ccgctgggtc gccagcgaaa tcgacgaaca cggccggcgt ctccaccggc gcggcgccaa
     7261 ggtcgtcata gacgacgacc tggtcatagt agccgagacc ctcgacgaag gccctgttcc
     7321 gcggcgaggt cagcccgacc accttgaccc ggccggaccg cttcagcagc caggccaggc
     7381 cgagggcggt gcggctggac gcgctggcca ggatcacggt cctggcgccg aagtcgtcgt
     7441 tgtcgaccag caggtcgtcg atcaggaacg aggtcatgaa cagcggattg agcagcgatc
     7501 gctggtcctg cagcgtcgcc tcggccggcg cggccacata ctggttgtag gtcggtggca
     7561 ggtcggcccg gtgggcggcc tcgtcgacga aaccggcttt cgaacggcga ggcgtcgcca
     7621 gcagatggct gcccatcggc cagtagccgt agaaccgctc gccgggcgcg aagtcgggat
     7681 gaccgctggc ctcgaccacg ccgtagcccc aggtggggat gcagccccag ccgtcggcct
     7741 tggggaagaa ccgccagtag ccgatctggt cgccggccac ggcgtaggtg acgttgttgg
     7801 ccgtcagggc gaaggactcc acgcggaaca gcacctcgcc cgcggccggc gcagtcaggg
     7861 cgaccggcgc gatccgggtc tcgtggaggt catttcgacg tacgagaaag tcccagccct
     7921 gcgccgccat gtccggtgtc cttgcctgat gatgcgcgta cactgtaaat ctgacccgta
     7981 gccagagtcc aacggtccat gccccgcctt cttaccgaca gcgacgtcgc cgacttccgc
     8041 gagcgtctga tcgccgcggc cgagcgactg ttcggcgagc gcggccccga cggggtgacc
     8101 atgcgccagc tggccgccga gctgggggtc agcccgatga cgccctatcg ctatttcaag
     8161 gacaaggacg cgatcctcgc ggcggtgcgg gccagcggct tcgcgcgctt cgccgaggcg
     8221 atggagcagg cgaccgccgg gatcaccgat ccgcagcagc agtcggaggc cacgggccgg
     8281 gcctacgtcc ggttcgcgct cggcaatccg gacgcctacc ggctgatgtt cgacttctcg
     8341 cagccgaacg aggccgacta tccggaactg ctcgaggcct ccgcccgggc caggacgatg
     8401 atggcgcggc acgcccaggc gttggtggcg tccggcgagg cccggggcga cccgatgatg
     8461 atcgcccaca tcctctgggc ggcgctgcac gggggtctgg tgctgcagat ggccggcaag
     8521 ctgtcgccgg atatcgaccc ctcggccctg cgtcgccagg ccttcgagac catcctgcgc
     8581 ggcttgaagc cggacgcttg acggtcgctg gggaagggcg aggactgttc gtgcgtcctt
     8641 cgacacggcc cttcgggcct gctcaggatg acgtaagggg ttggcgctga accacgtcat
     8701 ggtgagcagc gcgaagcgcg tgtcgaacca cgcaagttcg gagcagatca tgacccagcc
     8761 caagcccttc accgtcgact tgtccgagtt cgaggtgcgt cgcgtgctcg accaggtgcg
     8821 gcactatccc tggccgccgg cgccggcggt cgatgacggc tggctctacg gctgcgacgc
     8881 gaccttcctc gaagagacct gcgcctactg ggccgaccac tacgactggc gggccgccgt
     8941 cgccgagctg aaccgcttcc cgcagttcac cgcccgggtc gaggagttcg atatccattt
     9001 cgtccatgtg gtcggcgagg ccggcggcaa gcggcccctg ctgctgaccc acggctggcc
     9061 gggcagccac tacgagttct gggcttcgat cgggaagctg gcctttccga gccggcatgg
     9121 cgggaaggcc gaggacgcct tcgatgtggt ggtccccagc ctgccgggct tcggcttctc
     9181 ctccaagccg gcgcggcccg tcggccagcg gctgacggcg aagctgttca acaccctgat
     9241 gaccgaggtt ctgggctacg accaatacct ggcgcagggc ggcgactggg gcgggctggt
     9301 gacctcgtgg atcggcttcg accacggcgc cccctctgag ggaaagaagg gggcgaaggc
     9361 gatccacctc aacatgatcg gcttccggcc caagggcggg ccgcagtcgc cggaggaggt
     9421 cgcctggatc acccgccagc aggcgatgat gggcatgctg ggcgcctatt tcggcctgca
     9481 ggcgtcgaag ccgcagtcgc tggcgtggct cggagccggc aatccggtgg ggcaggcggc
     9541 gtggatcctc gagcggtttc atgactggtc ggacctgcgc agcaagccgc tgatcggcgc
     9601 ctacacccgc gaccagctga tcaccaatgt gatgatctat gtgatgaccg gcagcttcac
     9661 gaccggcgcc tggtactatc gcggcctgat ggaggagggc ggcatcgcgg ccatggaggg
     9721 ccagcgctgc gagaccccga cggccttcgc caacttcccc ggcgagccgc tctacaccgc
     9781 tccgccgcgc agctgggccg accgcgccta caacatcacc cgctggaacg acatgcccag
     9841 cggcggccat ttcgccgcga tggaagagcc ggacctgttc gtggcggatg tgcgggactg
     9901 ggccgcttct ctctgacacc caatactgtc atcccggccg aagcgaagcg gagagccggg
     9961 acccagattc gaccgagcct tgatctgggt cccggatcgc cttcggcgtc cgggatgaca
    10021 gtgagattta ggacttgggc ttcggcttca gcagcagcag cgccgccaga ctgagcgcgc
    10081 acgctccgct gaggtagaac ccgacgtagg tcaggccgcc ggccttggcc aggccctcgg
    10141 cgatcagcgg ggtcatgccg ccgccgatga tgccgccgac gttgaaggcc acgctggcgc
    10201 cggtgtagcg cacccgcgcc gggaacaggc cgggaagcca ggccccgagc gggccgtaga
    10261 ccagccccat gagcaccatc gccaccgaca ggaaggcgcc gaccagcaat agcgagcctg
    10321 accccagcat gggcgccagg ccgaagcccg ccagcaccgc cagcacgcag ccgaccatca
    10381 gcacccggcg cgggttgctc gcgtccgacc aggaaccggc ccagatgatg ccgccggcca
    10441 tgaacaggat ggcgatcagc tgcacgccga ggaacgcctc ccggctgtag cccagcgtct
    10501 tgaccccgta gcccagggcg aaggcggttg agaggtagta gatgcagaag caggccacca
    10561 cgccgaaggt gccgccgagc acctcgcgcc agttggtccg catcacctcg cccagcggca
    10621 cggccggcgg cggggcctcc tccagcgcgg ccttgaaggc gggcgtctcg gtcaggctca
    10681 gccgcaccca cagaccgatg gccaccagca ccgcgctgag caggaacgga atccgccagc
    10741 cccagctgag gaactgctcc ggcgacagga ccagaccgag gatcaggaac aggccgttgg
    10801 cggcgatgaa gccgaccggg gcgcccagct gcgggaacat gccgaagcgg gccctccagc
    10861 cgggcggggc gttctcgacc gccagcaggg ccgcgccgcc ccattcgccg ccgaggccga
    10921 agccctggcc gaagcgcagg aggcacagca gcagcggcgc gatccagccg gccatggcgt
    10981 agccaggcag gaaggcgatc gcggtggtcg agccgcccat cagcagcagc gaggcgacca
    11041 gcgtcgattt gcgcccgatg cggtcgccga aatgaccgaa gaacaccgcc cccacgggcc
    11101 gggcgatgaa ggcgatggcg aaggtggcgt agctgagcat cagctgcgcc gaggccgagt
    11161 ccttgggaaa gaacagcggc ccgaacacca gcgaggcggc cgtggcgtag atgtaaaagt
    11221 cgtagaactc gaccgccgtg ccgaccaggc tggcggtcag cacggtgcgg ttggagggcg
    11281 cggcgcccgc ctgcacagtc gtcgtcatgg tgtcccccgt ttccctgtcc ctgaatggcg
    11341 gggtgaaggg cggcgggtca agcggtgcgc ggttcgggcg tgcgtccttc gacacggccg
    11401 ctgcgcggcc tgctcaggat gacgttgagg ggttggtgct ggaccacgtc atggggagca
    11461 gcgccgaagg cgcgtgtcga accgcgcacg cttttcatct gtgctatagc ccgccgcatg
    11521 accgacacgc ccctgacccc cgccgagaag atgaaggcca tcctggccgc caaacagggc
    11581 ggtctcgctc gcgccggcca gggcaacgcc gcctcgcgcg gcaggcatgg cgagagcatc
    11641 gccgccgccc ggtcgaagtc gcaatccaag ccggccctgc ggaagtagtc ccggtggccc
    11701 gctacgactt cgcctccgac aacgtcgccg gggccatgcc cgaggtgatg gacgccctga
    11761 tcgccgccaa cgcaggcacg gcctcgggct acggaaccga ccatatcagc cggatggccg
    11821 ccgacctgat ccgcgccgcg cttgacgccg acgccgaggt tcggttcgtg gcctcgggca
    11881 ccgcggcgaa cgggctgtcc tgcgcggtcc tggcgcagtc gcacgaggcc gttctggcgc
    11941 atgagcatgc ccacatcctg accgacgaaa ccggcgcccc cggcttcttc ggcgcgggcg
    12001 tcgggctgat cggcctcccc ggcgccagcg gccgaatcga tccggcggcg ctggccgccg
    12061 cccttgccca gcccgacctg tcctaccgcc agtcggcggc ggccctgtcc ctgaccaaca
    12121 ccaccgagta cggcaccgtc tgttcggtcg agagcttcaa ggccctgatc gatccggtga
    12181 aggcgagggg ctacggcgtg catctggatg gcgcgcggct ggccaacgcg gtggccgccg
    12241 gcttcgacct gaaggccgtc ccgaagctcg gcgtcgatat cgtgatcatg ggcgggacca
    12301 agtccggctc gaccccgacc gaagccatcg tcctgctgaa caaggcgctc acccgccggt
    12361 tcgatgcgcg gctgaagcat gccggccagc tggtttccaa gggacggttc ctcgccgcgc
    12421 cgtggatcgg cctgctggag accgacgcgt ggcggacccg cgccgcccac gccaacgcca
    12481 tggcccggcg gctggcggcg ctgatgccgt tcccgatcgt gcatccggtc gaggccaacg
    12541 gcgtgttcgt cgagatggac cagccgacgc tggaccggct cgccgcgacg ggctggttcg
    12601 tctaccgctt catcgacgac acggtgcgct tcatgtgcag ctgggcgacc acgccggaga
    12661 tggtcgacga gctgggagag gcgctcgctt ccatcaaatg atccctcatg gcccggctcg
    12721 tccgggccac ccaaggactc gatcattcag aacccgctcg ccccttccgc gacatccaac
    12781 ccacggcggc gtgcttgggt ggcccggacg agccgggcca tgacggagtg caattggaac
    12841 gcgccgccct cttgcctcac ccaacgtccg cccgttagct cgcggccatg agcgagccat
    12901 cccatcccga aatccgcgag gccgtccgca agctgtgcgc cgggttcccc ggcgagtact
    12961 ggcgcgcgct tgaccgtgag cggacctatc cgaccgaatt cgtgcgcacc ctgacggaag
    13021 cgggcttcct gtcggtgctg atcccggagg aatacggcgg ttcgggcctg ggcctttcgg
    13081 cggccaccgc ggtgctggag gagatccacc ggtcgggctg caacggcggc gcctgtcacg
    13141 cccagatgta caccatgggc acggtgctca agcacggcag tccggcgcag aaggaggcct
    13201 atctgcccag gatcgccagc ggcgagatcc gcctgcaggc tttcggcgtc accgaaccga
    13261 ccgccggcac cgacaccacc cgcatctcca ccttcgcccg ccgcgagggc gacaaataca
    13321 tcgtcaacgg ccagaagatc tggatcagcc gggccgagca cagcgacctg atggtgctgc
    13381 tggtgcggac cacggcccgt gaggacgccg cccgacccgc cgacggcatg agcgtgctgc
    13441 tggtcgacat gcgcgaggcg gtcggcaggg gcctgaccat ccggccgatc cgcaccatgc
    13501 tgaaccacgc caccaccgag ctgttcttcg acaacctcga ggtgccggcc ggcaacctcg
    13561 tcggcgagga gggcaagggt ttccgctaca tcctcgacgg catgaacgcc gaacgcatcc
    13621 tgatcgcggc cgaatgcatc ggcgacgggc ggttcttcat cgaccgctcg tcggcctacg
    13681 ccaaggaacg caacgtcttc ggccgggcca tcggcgagaa ccagggcgtg cagttcccga
    13741 tcgcccgcaa ctacgtccag ctgtccgccg cggccctggc ggtggatcac gccgcggccc
    13801 tgttcgacgc cggcaagccc tgcggcaccg aggccaacat ggccaagatg ctggccagcg
    13861 aggccagctg ggccgccgcc gacacctgca tccagaccca cggcggcttc gggttcgccg
    13921 aggagtacga catcgagcgg aagttccgcg agacgcgcct ctaccaggtg gcgccgatca
    13981 gcacgaacct gatcctcagc cacgtgggca cccacgtgct gggcatgccc aagagcttct
    14041 gacacaaggc cccgtgccga ccttcatccc catcgacgat cccgccgacc cccggcttga
    14101 ggcctatcgc gacatccgcg agcgggacct ggtcgggcgg caggggcggt tcatcgccga
    14161 gggcgagacg gtgctgcggg tgctggcggg gcaggaccgg ttccggccgc tgtctgtgct
    14221 gatcgcgccc aagcggctgg agaaactggc gccgatcctc ggccagctgg cggacgacgc
    14281 gccggtctac ctggccggcc agtcggtgct ggacgcgatc gtcggcttcg agctgcaccg
    14341 gggcatcctg gcgctggccg aaagggcggc gccggtcgcg gccgacgacc tgctcgcagg
    14401 cctgccgggc aaggcgctgg ttctggcgct ggtcgggatt tccaaccacg acaacatggg
    14461 cgggctgttc cgcaacgccg ccgggttcgg ggtcgacgcg gcgctgctcg acccgacctg
    14521 ctgcgacccg ctgtatcgca aggccatccg ggtgtcggtc ggcggggtcc tgcgggttcc
    14581 ctacgcccgc cttggcgagg gcgaggacct tgtcgatcta ctggagcagc gcggcttcac
    14641 gccggtcgcc ctcagcccgt caggcgacac cgcgctgtcc gacctgccgg ccctgtcccg
    14701 tccggccctg ctgctgggcg cggaagggcc agggctgccg gcggcgaccc tggcccgctg
    14761 ccggacggtc tcgatcccga tggcgcccgg cttcgactcg ctgaatgtcg cggtcgcggc
    14821 cgggatcgcc ctccaccagc ttcggtcggc ccgaagctag ttagcccaca tcacgtattc
    14881 ggcgtccttc ggcttcaccg tgccgtcggc cggctggtcg atccagatcg ggccctcgat
    14941 cagcgccggc cagatcggct tggccgcgcg gcgatcctgg gcgtcgagca tcgccgtcag
    15001 ctgggccacg atcgccggat tggccgtcgc gacattgctc tgctcggtcg ggtcgctctt
    15061 gagattgaac agccaggctt tcttcggccg ctccgacagc tgcagcttcc agtcgccgtt
    15121 gatcaccacg cgatagtggc ctgaacggaa gaagagcggc cggtcggaga cctgtcccct
    15181 ggacagggcg accaggtcga cgccgtccag cacccccggc ccggccggcg gcgcatgggc
    15241 cgccgccgcc gccgtggcga agatgtcgaa gtgcatggcc ctggccggcg tcgtcgtgcc
    15301 gggctggatc tggccgggcc agcggacgaa gaacggcact cgcaccccgc cttcgaagaa
    15361 ggtcgccttg aagccgcggt agggtttgtt gatgtccggc aggccgatgt agttggcccc
    15421 gccgttgtcg ctggtgaaga tgacgatggt gtcgccctcg aggcccttgg ctttcagctc
    15481 ggccagcacc ttgccgacgt tgcggtccag cgcccggatc atcgccgcat agacgcgcag
    15541 gcggtggtcc ttgatctgcg gcagggcgtc atagtccgac ttgagcgcct gcagcggcgt
    15601 gtgcggcgcg ttgaaggcca ggtacatgaa gaacggccgg ttggcgttgg cgtcgatcgc
    15661 ccgcacggcc tcgtcgccga agtagtcggt gatgtactgg ttcggatgga acggcttgct
    15721 gccgtcgaag cgcaccgacc agggcaggtt ggcccacagg aaacggtcaa ttggatcgaa
    15781 gtcctgcttg gagttcacga cattcgggtc gttggcctcc atgtacatgc cgccgccggc
    15841 gaggatgttg agggtgtcgt cgaagccctg gttctccggc cgcatgccgt cggcctcgcc
    15901 caggtgccac ttgccgatat gcagggtgcg gtagcccgcc gccttcatgg tctcggccag
    15961 ggtgacctcg ctcgccggca cgcccagctt ctccaccggc ggcatgtccc tcacgcgatc
    16021 agcgtagaac accggcctga cgccatcgtg tccgcccatg cgggcgatgt tcttggcgaa
    16081 cgccaccggc acgccggtga actcgaagcc gaagcggatc ggatagcgac cggtcatcat
    16141 cgccgcccgc gacggcgagc aggtggcgtt tccggcgtag ccgttggtga aggccacgcc
    16201 atcgcgcgcg agcgagtcga tgttgggggt cggtacggct ccgttcgcca cgccctggcc
    16261 ggtcgactgc aggtcgttga tgcccaggtc gtcggcgacg atgacgatga cgtttggcgg
    16321 ccgcttcgcc ccgggggcca gggccggcgc ggcgcccgag gcccaggtca cctggtggta
    16381 gggctggatc gggttcttga tccgcccgat cacgccgggg atgtagatcc agaaatgctg
    16441 gaaggcgatg aagccggcca cgaggacgac gactatggac ccgagggtcc aggcgagttt
    16501 gcggttcacg gtcgatcctc cggtcaatgg agcaggctgg agcgggtgat ggtcttgccg
    16561 ccgacgggca ggctgatcag gtcgccgtca cgggcgatcc acaagggtcc ggtgaacacc
    16621 ttgcgggcgt cgccgaggaa cggaccctcc agcgcgcgca gcggcagcgg cggcacgatg
    16681 tggttcagca gcagggcgcc gacgccgtcg tcgcgggcga tggccgccgc cgccgacggg
    16741 ctggtgtggt agttctggat gtccagcatg atctgggccc gtttggtgtt gccggccttc
    16801 agggaagcgt cccggatcag ggtcaccagc ggcgtcgaca gggcctcgtg gatcagcagg
    16861 tcggttcccc tggcatactt ggcgaccacg ggcgagggag cggtgtcgcc gctgatcaca
    16921 acgctgcggc ccttgtagtc gaagcggtag ccgacggcgg gctcgaccgg cccatggtcg
    16981 accctgaagg cggtgatctt cacaccgtcc gtttcgagca cgacgacgga atcctgcccc
    17041 tcggggatgg cgaacggccg ggcctgaacg cccgcgcccg acggcggcat gatcaccggg
    17101 ccgtggtggg cggtgcggta gccggcgtcg agcgcatagg cctcggtgaa gccggcggcg
    17161 acccgttcga ctccgggagg tccgtacagc tgcagcggcg tggtcaggtt gccgccgccc
    17221 cagcgttgca gggccacgtt gcccaggccg tcgatgtggt cggaatggaa gtgggtcagc
    17281 agcacgaccg agtcgccgcc gatgcccatg cggcccaggg tgcggccggc ggcctcgccc
    17341 gtgtcgacga tgaacacgcg cttgccggcg atgaccgccg tgcagggtcc gccgcggtcg
    17401 ccgtccggga acgggccgcc cgcgccgcag atcgccacgt gcaggccgtc gggcagcgaa
    17461 gggacgaccg gatgcgccat cgccgtctcc aggcgacgct ccatgagccg cacggcgatc
    17521 ttgccgcgga ccgcccaggc gcccgccgcc agcaggacca gcaccagcag cagcccggtg
    17581 aacacccgtt tcatgtcgcc ccccgaattc cggcttgccc gcccgaatta tattggcata
    17641 gtttgtgcca ttagttttcc cgccggtcaa gccggccgcc gatcacctgg agagcccgcg
    17701 tgcaggtcct gagaacgccc gacgaccgtt tcgcaaacct gccggacttt ccctacagtc
    17761 ctcactacga gacgatccgc gacccgtcgg gcgtggatct gcgcatcggc tatatcgatg
    17821 agggtccgcg agacgccgcg cccgtgctgc tgatgcacgg cgagccgtcc tggtcttacc
    17881 tgtaccgggg gatcatcgcg ccgctgaagg accggcacag ggtcgtggcg cccgacctgg
    17941 tgggtttcgg ccggtccgac aagccagccg aaaagaccga ctacagctac gagaagcacg
    18001 tcgcctggat gagcgcctgg ctgctggcgc tggacctgaa cgacatcacc ctgttctgcc
    18061 aggactgggg cgggctgatc gggctgcgcc tggtcgcggc cttccccgag cgattcgcgc
    18121 gggtgatcgt ggccaacacc ggcatgccca cgggcacggg catgagcgag ggcttcaaga
    18181 cctggctgac catgagccag accatcccgg tcttcccggc cggaatgatc gtcaacatgg
    18241 gctccctgcg cgaactgtcg ccggacgaga tcgccgccta tgacgcgccc tatcccgacg
    18301 agagctacaa gtccggcgcg cggatcttcc cgaccttcgt gcccgtgacg cccgaccatc
    18361 cgtcggtcgc cgagaacaag gccgcctgga ccgtgctgga gcggttcgac aagccgttcc
    18421 tgacggcctt cagcgacggc gacccggtct cgaagggcgg cgaccggccg ttccaggagc
    18481 gggtgcccgg cgccaggggc cagccccaca ccaccatcga gggcggcggc cacttcctgc
    18541 aggaagacaa gccggccgag atcgccgccc tcattgaccg ctttatttcg gagacccgct
    18601 gatgaacgtc gagaacgccg tcaccccgac cggcgaccag ttgaaagccc tgctggcgct
    18661 gggcggcgac aggccgatcg tcatggtcaa cctgctcaag ttccgcgaga aggcccagta
    18721 cgacgagccg ggcgagccgg agatgaccgg gcgcgaagcc tacgaccgct atggccgcgt
    18781 gatgggcccg ctggtggcgt cgaagggcgg ccggctgcgc ttttcgagca gggtcgacgc
    18841 cctggtgatc ggcgaggtcg gcgagctgtg ggacgccgtg gcgatcatgg aatacccgag
    18901 ccgggcggcc tttgtggcca tcgccacgtc gccggaggtc caggagatcg gtcggcaccg
    18961 caaggctggt ctggccggcc agctgctgat ccagtgctcc gaggcgggcg tgtgatgccc
    19021 gccgaggccc gggctccgtc acggattacg cttggcctgc tggtcttgct gggcctgatc
    19081 aatctggtcc gcggcgcgat ccacgtgttc ctgccggaca gcggcgcggg cgtcatcgcc
    19141 catttcgagc tggccgaggg cgggcggaca atcgtcttcc tgctggcgat ggtcggggcc
    19201 ggccagatcg ggtcggggct gatcgacctg ctcgtcgtgg cgcgctttcg cgccttcgcc
    19261 gcccccctgc tggccataga gctgctgcgg gcgctgatcg ccctctatat cgccttcgtc
    19321 tcgaagtcgg ttggaaccgg ttatccgggc gccaggggca tcgtcggcgc ggcggtcgtg
    19381 ctgaccctgg cggtgctgtg ggagttcggc cgtcgcggcc gcaggggctg atcgcttgca
    19441 ggatgccaag gtgatgatag gcctgaccgc atgaccgccc ctcccaaacc gccgctggcc
    19501 gccgaccggg ttatcgacgg acgccggctg cggtccgagg ccagccggct gcgcatcgtc
    19561 gaggcgatga tcgccctggt gtcggaaggc ctggccatgc ccccggcaga ggcggtggcc
    19621 gctcgcgccg gcgtcgggct gcgcaccgtg ttccggctgt tcgaggacat ggacggactc
    19681 taccggggca tccaggccgt cgtgaccgag cggctcggcg gcctgctgga gggaccgatc
    19741 gaggccgccg actggcgcca ggccgtcgac atcctgatcg atcgccgcgc ggaggcgttc
    19801 gagatgatcc tgccgctgca gatcgccgcc gacagcccgc gcacccggtc cgccgccctg
    19861 cgcgaggggc ggctgcggct ggtgcaggcg cagcgggata ccttgctggc cgtgcttccc
    19921 gcgtcggtcc aggcggacgg ccggctggtc gaggcgctcg acctcgcggt cagcttcgag
    19981 gcctggcggc ggttgcgcac cgatcagcac ctggacagcg acgccgccag ggcggtcatg
    20041 catcgcctgg cccaggccct gctccacggt ctggcgtgaa gcagctcgtt ctcctgctgg
    20101 cgctcggact ggcgggctgt ggagcaaagg ccccgcaact ggccgcgccg acgcgcgcct
    20161 gtcccggccc cgcgccgctc ggcgaagtcg cgctgaaggg cgggaccttc acccttgggg
    20221 ccaatcccga gcacattgac gaggggccgc cgcgcaccgt gacggtggcg cccttctcga
    20281 tcgaccgcac agaggtcacc aacgccgagt tcgccgcctt cgtgaaggcg accggctacg
    20341 tcaccctggc cgagcggcag ccggatccgg ccctctatcc gggcgttccc gccggcaagt
    20401 tgaagccgtc gtcgctggtc ttcgtcggcg ccgatgccct caacgacggc aacccgatgc
    20461 agtggtggcg tatcatcgac ggggccagct ggaagcatcc ggagggaccg ggcagcgacc
    20521 tgaagggccg cgagcgccat ccggtggtgc aggtggcgta tgacgacgcc ctggcctatg
    20581 cgcgctggaa ggggcgcgac ctgccgaccg aggccgaatg ggagttcgcg gcccgggccg
    20641 gtctcgacgg cgcccgctac gaatggggcg acgacaagcc ggtccccggc cggccgcgcg
    20701 ccaacagctg gcagggggtg ttcccggccg tcgacatcgg cgacgacggc tacaaggccc
    20761 gcaccgcccc ggtcggctgc tatccggcca gcgcctatgg cctgtacgac atgaccggca
    20821 acgtctggga gtggacgaag gactggtacg agacgcccaa cccgaacggc cgcagccacc
    20881 tgatcaaggg cggctcgttc ctctgcgccg acaacttctg ctaccgctat cgcccgagcg
    20941 cgcggcagcc gggaccgccg gacacgggga ccagccatgt gggattccgg acggtgaagc
    21001 ggggatagtg cttccctgcg tccttcgaca cggccccggg ggcctgctca ggatgacgtg
    21061 gaggggtgcg cttcaaagtc tacgtcatgg tgagcagcgc cagaggcgcg tgtcgaacca
    21121 cgcaacgacg cggcagcctc ctcaactggt tgcacgcccg ggttccaggt tcgagaatgg
    21181 ccgcaacagg cagggggaat gcatcgatgg gcggcatcac tgtccagaag gtcgccgggg
    21241 cgctgggcgc ggagatcggc ggggtcgacc tgtccgcgcc gctcgacgac gagaccatcg
    21301 ccgcgatccg ccgggcgctt gtcgagcatc aggtgacctt cttccgcgac cagtcgctga
    21361 cgccggagca gcagctggct ttcggccgtc ggttcgggcc gctcaacatc cacccctatg
    21421 tcgccggcat ggccgaccat ccggaggtga tggagatcat caaggagccg accgaccgga
    21481 tcaatttcgg cggcggctgg cactcggaca tgagcttcct ggagacgccg gcgatcggct
    21541 cgattctcta tgccgtcgaa ctgccggact ggggcggcga caccctgttc gccagccagg
    21601 cggcggcgtt cgaggccctg tcgccggggc tgcaggcgac gctggagggg ctgaacgcga
    21661 tccactcggc cgagcgtgaa tacgccccga cgggcccctc ggcgcagaag cgcgagtcga
    21721 tgaagatcag cgacgccgag ggcatggccg ggcggttcag ccacccggtg gtcaaggtcc
    21781 acccggagac cggccgcaag gcgctgtacg tcaatccggc cttcacgatg cagatcgacg
    21841 gctggagccg ccgggagtcg cggccgctgc tcaactatct gttcgagcac agccgccaag
    21901 aggccttcac ctgccgcctg cggtggcgcc cgggcacggt ggccttctgg gacaaccgct
    21961 cggtctggca ctacgcgctc aacgactacc ccggccagcg ccggcacatg cgccgggtga
    22021 cggtggatcc ggtggcggcc tagaccacct gcgtcgccgt ccccgccagt tatcaaagca
    22081 ctgtcatccc ggacgggccg aaggaccgat ccgggaccca gattggatct ctatcgccgt
    22141 cgaagccaaa tctgggtccc ggctctccgc tacgctacgg ccgggatgac agtgattggg
    22201 aaggagattt ttctggtgac a
//
LOCUS       DAORNX010000004        21451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_1897_length_21451_cov_8.153627, whole genome shotgun sequence.
ACCESSION   DAORNX010000004 DAORNX010000000
VERSION     DAORNX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 21451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21451
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_21451_cov_8.153627"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLO65_00380"
     CDS             complement(<1..417)
                     /locus_tag="PLO65_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD26734.1"
                     /translation="MIETPRLILRGWRESDLDPWAALNADPEVMRHFPATQTRIESDA
                     MAARNQDHIDQHGFGLWAVERREDGAFLGFTGLMVLRESNPLAPGVEAGWRLARHAWG
                     QGYATEAARAAMDDGFTRLGLKQVVAFTARPNLPSQA"
     gene            complement(414..1292)
                     /gene="aguB"
                     /locus_tag="PLO65_00385"
     CDS             complement(414..1292)
                     /gene="aguB"
                     /locus_tag="PLO65_00385"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HRD26735.1"
                     /translation="MARTLSLAAIQTSYGLDLDANIRKTEGFIREAAARGAQVILPSE
                     LFQGPYFCVAQEERWFATAHPWRDHPCVRALAPLAGELGVVIPISIFEREGPHYFNSL
                     VMADADGSLLGVYRKSHIPDGPGYQEKYYFRPGDTGFRVWDTRHGRIGVGICWDQWYP
                     ECARAMALMGAEALLYPTAIGSEPHDPSLDTALPWRRAMQGHAVSNIIPVVGANRTGF
                     EPWDGYPGGGQRFYGSSFIADHRGDLVSELGRDDEGLVSTTVDLDFLATHRAAWGFFR
                     DRRPDLYGALTSPRPA"
     gene            complement(1292..2290)
                     /locus_tag="PLO65_00390"
     CDS             complement(1292..2290)
                     /locus_tag="PLO65_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRD26736.1"
                     /translation="MSTPLVPAEWSPHRAMWLGFPSHAELWREDLDPAQDEVAALARA
                     LAGPGEERVRLMVCGDAAEAAARARLDGVAGVEIVRALFGDIWLRDTGPIFRGDGRAA
                     GFRFNGWGGKYQLEGDDQVAAQIAAASGAPLDANAFILEGGAVEHDGLGTVLTTRQCL
                     LNPNRNPGWTQGAAEAALAESLGARKVLWLGDGLRNDHTDGHVDNLARFVAPGVVACP
                     IAWGRGDPNTRLYDETARQLSAMTDARGGQLQVMRIPSPGWIDGDDGPAPASHMNFIV
                     ANGAVVVPLYDDARAGAFAVDAVQSLFPERKAIGLPSRAILTGGGSFHCITQQEPA"
     gene            complement(2400..4985)
                     /locus_tag="PLO65_00395"
     CDS             complement(2400..4985)
                     /locus_tag="PLO65_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26737.1"
                     /translation="MMKNKLAFVATSALVGSMLLASNALAQSTGTTEVEEVVVTATAG
                     QPNIDGVISVETAPKAKATIDQEFIATQPTGQTIIQTLNLTPGLNFTNNDPYGSSGGN
                     LRIRGFDGPRISLTFDGIPLNDTGNYAIYTNQQLDPELVSKASVNMGTTDVDSPTASA
                     TGGTINYTSRRPSADMGFVLNGAVGEFNYRRLFGMVDTGEIGPWGTTAYFAASTQQYD
                     KFKGPGELQKWQVNAKVYQPIGDNGDFISLAVHYNENRNDFYRTASMATFRANGMDYD
                     NNPTYLPIVSTPVALPGTGDVDADPADNSNFYGLRINPSNTGNIREQARFTLTPTLTL
                     TVDSAFQYVLANGGGSTTVSEVAAVGANTVVTGSTGGVLCGPGGTGGSGFDINGDGDC
                     RDTFVRFYSPSTTNTRRYTSMGSLIWEPNENNSFRIAYTYDRGRHRQTGQYGLINGNG
                     DPQDVFGGKDGYGQKVYNADGYALRSRDRLSYAILNQISADWRGRFMDDNLFVTIGVR
                     APTFKRELNQYCLTQNNGFSVLCTSAPTFANVDGDPTTAGVQPSDNLVTLNAAGTGTR
                     YIVPFSRDVSYDAVLPNVGVSYQVMPGHTLFISYAEGFSAPRTDNLYTAGVKTTSTDA
                     PIVLSNVEPETSQAWDVGYRYRSPTMIISTALWSNNFQNRIVSAFDPDLGFSVDRNVG
                     DVKLWGFDAQAGWTPNSTFSLYASVSYTNSELQDDLLLSALPTYLPTKGKALVETPEW
                     MVTGRAQWRVTDAFTVGLQGKHVGDRYTTDVNDEQTPGYNVFDLDLRYALPFGGEGTS
                     VQLNVTNIFDEEYFGNISSGNNAKPIANVNPDLSTPPVSKSASGAFVSVGAPRTVQFS
                     IRTRF"
     gene            5266..>6276
                     /locus_tag="PLO65_00400"
     CDS             5266..>6276
                     /locus_tag="PLO65_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRD26738.1"
                     /translation="MTPPDTAPPSADPRQPLRMALALVLVLTVLRLAAIFASPLELNP
                     EEAQYWLWSRTLDFGYFSKPPMIAWAIWATTAIGGDSEAWVRFSAPLFHAVAGLSLFA
                     AGRRLYDDWTGLAAAALYLLMPGVALSSFILATDAPLLCFLALALLAYVTVRQGDHRL
                     APAAGLGAALGLAMLSKYAALYALIGIGLHLALSKDARRAWDLRTAAVALVAFAAVLA
                     PNLVWNANHHFATVGHTAANANLGGKLFNPGEMLGFLGGQFGVFGPVPFGVLAGGAAV
                     LAWRRRLQPQDLLLLCFAVPPLAVVTLGALLSRANDNWAASAYVAGAVLAAALLVRWR
                     ARW"
     assembly_gap    6277..6293
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6294..6846
                     /locus_tag="PLO65_00405"
     CDS             <6294..6846
                     /locus_tag="PLO65_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26739.1"
                     /translation="GGLGLQAALVVFMLLAVANPAIVDALGRSNDIKRARGWKQMAQQ
                     VVQRAQVEQINGGLSAIAVDDRFLFNALAYYGRDYIGTPDAPPLAIWLRRSHAGNQAE
                     TVAPLTPALGRRVLVVSLEGLQGQRIAADFATSRTVGVLRQILSVKCAARYRDDERKF
                     NHRCLRRETLILGEGFAPVKNRD"
     gene            complement(6926..7567)
                     /locus_tag="PLO65_00410"
     CDS             complement(6926..7567)
                     /locus_tag="PLO65_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRD26740.1"
                     /translation="MAWTREDMAARAARELRDGFYVNLGIGIPTLVANYIPEGMHVTL
                     QSENGMLGMGPFPYEGEEDADLINAGKQTITELDSSSYFSSADSFAMIRGGHIALSIL
                     GGMQVAENGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVVMDHCEKTGAPKLLHRCNL
                     PLTGAGVVDLLITELGVFEINRGRTPVRLIELAPGVTVDEVKGKTEAAFEVAV"
     gene            complement(7567..8277)
                     /locus_tag="PLO65_00415"
     CDS             complement(7567..8277)
                     /locus_tag="PLO65_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRD26741.1"
                     /translation="MADKVAKDAVEALSGLLFDGMTIMAGGFGLCGIPENLIAAIRES
                     GVKDLTVVSNNCGVDGFGLGILLENRQIRKMISSYVGENKLFEQLYLSGDLELEFNPQ
                     GTLAERIRAGGAGIPAFFTRTGVGTLVAEGKEVREFDGERYVMERGLTADLSIVKAWK
                     GDAEGNLVYRKTARNFNPMMATAGKATVVEVEQLVAIGDLDKDNIHTPGIYVDRLFRG
                     AVFEKRIERVTTRVKEVA"
     gene            8373..8939
                     /locus_tag="PLO65_00420"
     CDS             8373..8939
                     /locus_tag="PLO65_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRD26742.1"
                     /translation="MEAEGREPSVLLVFAHPALERSRANAVLAGAGRSAAGVRFHDLY
                     EVYPDFVIDVEAEQQRLLAHQVIALQFPFYWYSTPALMKEWLDLVWLHGFAYGKGGDR
                     LKGKQLFVACTTGGPAQAYHQRGYNRFTMDEFLRPLEQTAALCGMTWETPFVVHGARL
                     KDEAQIRGEADRYRSRLEGLAAAARETV"
     gene            8939..10840
                     /locus_tag="PLO65_00425"
     CDS             8939..10840
                     /locus_tag="PLO65_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRD26743.1"
                     /translation="MHGDVLTQALVYLAAGVVTVAAAKKLGLGSVLGYLIAGAVIGPF
                     ALNLTGDPEDVMRFAEFGVVILLFLIGLEVRPALLWSMRKTIFGLGGAQVAATALAIA
                     GAGMILGLPWPTALAAGLVLAMSSTAIVLQTLDEKGLRQGPVGRAAFGVLLLQDLSII
                     PLFALLPLLATGPVTLTADTAHSTSLVADLPAWMQALAVLAAVGAVVGGGRFVVRPVF
                     RFIAQARLREIFTASALLIVVGVAALMQIVGLSPALGAFLAGVVLAESEFRRELETDI
                     EPFRGLLLGLFFITVGATINFALILREPLLLIGLVLGLMALKFAVMFALARVGGASNR
                     LAATVAVALAQGGEFAFVLLTFTVGAGVINQALADLLTAVVALSMAMTPLTVVAYEKI
                     LTRLDRGRPGETIADPTFEDADAEPDIIIAGFGRFGQVTGRLLTANGFKSTVLDADIE
                     QIDLLRRFGRRVHYGDATRLDLLRQAGAERARMLIVAIDDQEKAAELVERAREAFPDL
                     TILARAFDRRHAYDLLASGADAVERETFEGALAMGVTALKALGFRAHRAQRAADYFRR
                     HDRRTFDELRPMWGQTEAYLLASRDAAQTMDRLLQADLAKMKPGAADGWDTSGRDEEQ
                     RETAMGGED"
     gene            10916..11851
                     /locus_tag="PLO65_00430"
     CDS             10916..11851
                     /locus_tag="PLO65_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRD26744.1"
                     /translation="MPQSAIAPVSFAGYAADFDAFAEALGASFRRYGFAVLSDYDLDQ
                     ARIDAAIDAAKRFFALPEDVKKQYAVGKGGQRGYIPFGIETAKGADHFDLKEFWHMGR
                     DLPPGHRFEAQMPPNVWPAEVADFHEKVAWLYNALDAMGVKVLQAIARYLGLPRDQFD
                     AAVENGNSILRLLHYPPIPADATGVRAGAHGDINAITLLLGAEEGGLEVLDRDGRWLP
                     INPPPGCLVINIGDMLERATNDVLPSTVHRVVNPPPERRAFPRYSTPFFLHFAPDYEI
                     RTLPGCATADNPDRYPEPITADAFLVQRLKEIKLL"
     gene            11924..14350
                     /locus_tag="PLO65_00435"
     CDS             11924..14350
                     /locus_tag="PLO65_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD26745.1"
                     /translation="MAPSDAARLTEIRSLDLDALRGVIDRVTRLARGASGAVHAYVVL
                     VEEDHVWHSGFDGMPEHIGPSSDSTTARHIADPDTLWSEDLARDWPDHPWVSGPPHAR
                     GYANAPIILPGGLRLGGLCLIFGEPRPRDAGVVATLNDLAALLAEAIERLRAQRRTEA
                     AETISRAATDLKDAIMRSSPVALGMTDRDLRYLYVNEQWELEKGITSAEALGRTMAEL
                     FPRSYPLLAGSYEECLAGNTVSAERILVPSGRRPDRWLRATLCPWYEAGGEVGGLISM
                     SHDVSDVIGSLKMAERARKRLALALEIAEVLVYEVDYRAKTLKVDGASDTFFGGALTY
                     DDVARDMWVTVHPEDRAAAQAIWEDHLATGRPFRTEYRMNPPDGREVWAFSAAELLRD
                     DDGRITGVLGVLKNITSRKHVEEATVRARDLAEAANRAKSEFLANMSHEIRTPLNGVM
                     GVAAALSRTDLTPAQAEMVGLIETSGQTLEAILADVLDLARIESGRLELKPEPFDLDA
                     CLRSAAALFQPGADAKGLEFDLAIDAAASGTFIGDAVRLRQVVCNLLSNAVKFTSRGA
                     IGLSATVGADGGLAITVSDTGIGFDADVKERLFERFEQADGSITRRFGGTGLGLAISR
                     ALAEAMGGTLDARSEPGAGSSFVLTLALERSLEPLTAPARPDQPALADTRAPRVLLAE
                     DHAINRKVVELLFGGAGVELTSVENGAEAVAAVAGADFDLVLMDMQMPVMDGLTAIRA
                     IRAAEAAQGRPPTPIWALSANALPEHVAASLAAGADGHLSKPVSASALFDALENACAR
                     GQPEAIALNV"
     gene            complement(14402..16240)
                     /locus_tag="PLO65_00440"
     CDS             complement(14402..16240)
                     /locus_tag="PLO65_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HRD26746.1"
                     /translation="MQRAFKVLASGLTEPAVRFLRRFRSERGAVAIWFAVLALPTAVL
                     GFGLIDVSRASVEKRHLQDALDAATLMAARSSANTDETIQPIGAAALAAQLSGVSDAT
                     LSSSSFKVVGTKVVGTATAKVTPVIANLWLQGDMTIGAEAEVVRASTNLEVALVLDIT
                     GSMAGQKIRDVRSAAKDLVDMVVQDVQTPYYSKVALVPYSAAVNLGGYASGARGAIRG
                     PWTITDASWTTGSSRSISGVTKANPAVVTSNGHGFSNGDVVWITGVKGMTELNDRAFV
                     VANRTTNSFQLQGVKSSSYSKYKSGGTIRKCRVSDCSIVVTAAGHDLTDGEDVYISGV
                     SGMTELNDQTFTVANSTGSTFSLSGVNGADYGTYTSGGKADCTAPGCRYYAFTSAAGQ
                     PKMFEVSTCVTERTGLAAYTDVSPGGAPVGRNYPSSNNPCPDSVLTPLSTDKTMLKAQ
                     IDSYDDGGSTAGQIGLAWGWYSVAPDFGYLWPAASRPAAYGTPELLKVVVLMTDGDFN
                     TSYCNGVIARNAGPGSGGTSDKINCDATNGDAYDQASRLCANMKAKGVIIYTVGFDVR
                     AASLAAELMSDCATDAEHFYLPSSGEALKDAFAAIGRDIAKLRLSR"
     gene            complement(16330..17685)
                     /locus_tag="PLO65_00445"
     CDS             complement(16330..17685)
                     /locus_tag="PLO65_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD26747.1"
                     /translation="MKTGFLSPERLGRLLSAWRREDGAVAVQFAMVLLPMAVMVFGAL
                     DVSRGSAAKMRLQDALDAATLAAARSPATTDAELQAVGEAVLAADLTATEAVLRSSSF
                     RLDGSKVVATARASMTTVVANLWRDGDMTIGAESEVTRASNNIEVALALDVTGSMSGQ
                     KIADLKAAAKDLVDLVVQDQQSPYYTKLALAPYSAAVNVGTYADSVRGAYTAGTCTTP
                     GCASYKFTNASGGTRTFNISTCVTERTGTNAFTDAAPSSTFLGRNYPASGNPCLASTI
                     MPLSTDKSALKSRIDGLAAAGSTAGHLGLAWGWYLVSPNFASLWPTASQPAAYGADKL
                     LKVVVLMTDGAFNTTYCNGVISRDSGSGSGSSSDHVNCNAPNGGAFSQAQTLCANMKA
                     AGVIIYTVGFDVASDAAAQSIVQQCATDAEHVYLPSTGAALKDAFHAIGEDISRLRIS
                     R"
     gene            17847..18899
                     /locus_tag="PLO65_00450"
     CDS             17847..18899
                     /locus_tag="PLO65_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRD26748.1"
                     /translation="MDTLQLLLRGLAVGAVLTTGIGLLRSTHAGPARWVGAVFCLSVA
                     AFGVHSGGAETRALHLLGGPIWLLSVTATAWFWLFAVTLFEDRGVTLERLAPVGIMTA
                     VATVGAVLPRPASDGAWIVHNLLQVVLVSHVVRIIWLSWGGDLVEARRSLRGPFMVAV
                     AVYCIFLSGFEIAGTLGFHLGWGSLVQALSLAIMTLAGAATLLQGRPDLFESAVRRPA
                     AAAEAVAPQDRPDLARLQTIMADTDLWRREGLTIGQLASELGVPEHRLRRLINGGLGH
                     RNFADFLNARRIDAAKAALAAPDNARASISALAFELGYGSLGPFNRAFKDATGLTPSA
                     WRLRALAASPNPEKTG"
     gene            18990..19796
                     /locus_tag="PLO65_00455"
     CDS             18990..19796
                     /locus_tag="PLO65_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRD26749.1"
                     /translation="MTQTGIQLLDLGRHWLAGFQNDMVRYIVAAPLTWLVLCVICAPL
                     LRHRKIRPDSPPARQLVVEFLCSVRSVAVFSTVSLLSVLMWKAGLLWGDELREHAGPV
                     WIVVSFAVTVIAHDAYFYWAHRIMHDPRLFRRFHRRHHRSHNPTPFTAYSFDLAEAAV
                     MASFTVIWELVFPASWIGFTAFLIHQIARNVIGHSGYELFPANRRGRPLLDWMTTVTH
                     HDLHHAQAGWNYGLYFTWWDRWMGTEHPQYHARFAAVTAPRRAAALAAAE"
     gene            complement(19852..>21451)
                     /locus_tag="PLO65_00460"
     CDS             complement(19852..>21451)
                     /locus_tag="PLO65_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRD26750.1"
                     /translation="EAKAGAEITDLATRLAIEAARYDKVAVDPVTRRKLDILKRALVA
                     PAPQRPGAADEMATIASRLDSTYSTGKFTYNGKTLTLNDAEDILASSTDPAELKAVWE
                     GWHSISTVMRPDYARLVALSNEGSVALGYKDTGALWRSGYDMDPDAFAAETDRLWKQL
                     EPFYRNLHCYVRARLNEKYGDAVQPRTGPIRADLLGNMWAQQWGNIYPIVAPKSTTPS
                     YSLDELLVNAGYDPVKMVKTGEAFYVSMGLAPLPETFWTRSQITRPRDREVVCHASAW
                     DLDDADDIRIKMCTKVNAEDFYTVHHELGHNFYQRAYKNQPYIFKGGANDGFHEAIGD
                     FVGLSALTPTYLQQIGLLKDVPGEEGDIPFLLKMALDKIAFMPFGLLVDRWRWEVFSG
                     QLTPDQYNAGWWKLRTQYQGIVPPGPRPADAFDPGAKYHVPGNVPYTRYFLAHVYQFQ
                     FQRAACRQAGWTGPLHRCSIYGNKAVGAKFNTMLEMGASRPWPEAMAAFTGETQGDAS
                     AVADYFKPLNIWLTKQNKGEHCGW"
BASE COUNT         3078 a         7343 c         7491 g         3522 t
ORIGIN      
        1 cgcctgcgac gggaggttgg gccgggcggt gaaggccacc acctgcttca gacccaggcg
       61 cgtgaagccg tcgtccatcg ccgcccgcgc cgcctcggtg gcgtagccct ggccccaggc
      121 gtggcgggcc agtcgccagc cggcctcgac gccgggcgca agcgggttcg actcgcgcaa
      181 caccatcagc ccggtgaagc cgagaaaggc cccgtcctcg cgccgctcga cggcccacag
      241 gccaaagcca tgctggtcga tgtgatcctg gttacgcgcc gccatggcgt cgctctcgat
      301 ccgcgtctgg gtcgccggga agtgacgcat cacttcggga tcggcattga gcgcggccca
      361 ggggtcgagg tccgactcgc gccagccacg caggatgagg cgcggcgtct cgatcatgcc
      421 ggccgggggc tggtcagggc gccgtagagg tccggccggc ggtcgcggaa aaagccccag
      481 gccgcccggt gggtggccag gaagtcgagg tccacggtgg tcgacaccag cccctcgtcg
      541 tcccggccca gctcggagac gaggtcgccg cggtggtcgg cgatgaagga cgagccgtag
      601 aaccgctggc cgccgcccgg atagccgtcc cacggctcga agccggtgcg gttggccccg
      661 accaccggga tgatgttgct gaccgcatgg ccctgcatcg ccctgcgcca cggcagggcg
      721 gtgtcgaggc tcggatcatg cggctcgctg ccgatcgccg tcgggtacag cagcgcctcg
      781 gcgcccatca gggccatggc ccgcgcgcac tccgggtacc actggtccca gcagatgccg
      841 acgccgatgc ggccgtggcg ggtgtcccag acgcggaagc cggtgtcgcc gggccggaag
      901 tagtacttct cctggtagcc ggggccgtcg gggatgtggc tcttgcgata gacccccagc
      961 agcgacccgt cggcgtcggc catcaccagg ctgttgaaat agtgcggacc ttcccgctcg
     1021 aagatcgaga tcgggatcac cacgcccagc tcgccggcca gcggggccag ggccctgacg
     1081 cacggatggt cgcgccaggg atgggccgtg gcgaaccagc gctcctcctg ggcgacgcag
     1141 aagtagggcc cctggaacag ctccgacggc aggatgacct gcgcgcccct ggccgcggcc
     1201 tcgcggatga agccctcggt cttccggatg ttggcgtcca gatccaggcc gtaggacgtc
     1261 tggatcgccg cgaggctgag cgtccgggcc atcaggcggg ctcctgctgg gtgatgcagt
     1321 ggaacgaacc gccgccggtc aggatggcgc gcgacggcag gccgatggcc ttgcgctcgg
     1381 ggaacaggct ctgcaccgcg tcgaccgcga aggcgccggc cctggcgtcg tcgtagagcg
     1441 gcacgacgac cgcgccgttg gcgacgatga agttcatgtg gctggccggg gccgggccgt
     1501 cgtcgccgtc gatccagccg ggcgagggaa tgcgcatgac ctgcagctgg ccgccgcggg
     1561 cgtcggtcat ggccgacagc tgccgcgcgg tctcgtcata gagccgggtg ttcggatcgc
     1621 cccggcccca ggcgatcgga caggcgacca cacccggcgc gacgaaccgg gccagattgt
     1681 cgacatggcc atcggtgtga tcgttgcgca ggccgtcgcc cagccacagc accttgcgcg
     1741 cgccgaggct ttcggccagt gccgcctcgg cggccccctg cgtccagccg gggttgcggt
     1801 tcgggttgag caggcactgg cgcgtggtca gcacggtgcc aaggccgtcg tgctcgacag
     1861 ccccgccctc gaggatgaag gcgttggcgt cgagcggcgc gccggaggcg gcggcgatct
     1921 gggccgcgac ctggtcgtca ccctcaagct ggtactttcc gccccagccg ttgaagcgaa
     1981 agccggccgc gcgcccgtcg ccgcggaaga tcggacccgt gtcgcgcagc cagatgtcgc
     2041 cgaacagggc gcggacaatc tccactccgg ccacgccgtc gaggcgcgcg cgggcggcgg
     2101 cctccgctgc gtcgccgcag accatcagcc gcacgcgctc ctcacccggc ccggccaggg
     2161 cgcgggccag ggcggccact tcatcctggg ccgggtcgag gtcctcgcgc cacagctcgg
     2221 catggctggg gaagcccagc cacatggcgc ggtgcgggga ccattctgca ggaacgaggg
     2281 gcgtgctcat gggtcggggg aagtgggcct gacagggggt tgcgtcaagt gcggcggaaa
     2341 cgagaaaggg cggcccgatc gctcgggccg cccttccctg aagcgcagga cgcttcggac
     2401 tagaagcgcg tgcggatgct gaactgcacc gtccgcgggg cgccgacgct gacgaaggct
     2461 cccgaggccg acttggagac cggcggggtc gacaggtcgg ggttcacgtt ggcgatcggc
     2521 ttggcgttgt tgcccgagct gatgttgccg aagtactcct cgtcgaagat gttcgtcacg
     2581 ttcagctgca ccgaggtgcc ttcgccgccg aacggcaggg cgtagcgcag atccagatcg
     2641 aacacgttgt agccgggggt ctgctcgtcg ttgacgtccg tggtgtagcg gtcgccgacg
     2701 tgcttgccct ggaggccgac ggtgaaggcg tcggtcacgc gccattgggc gcgaccggtg
     2761 accatccatt ccggcgtctc gaccagcgcc ttgcccttgg tcggcaggta ggtcggcagg
     2821 gcgctcagca gcaggtcgtc ctgcagttcg ctgttggtgt agctgaccga ggcatagagg
     2881 ctgaacgtcg agttcggggt ccagccggcc tgggcgtcga agccccacag cttcacgtcg
     2941 ccgacgttgc ggtcgacgct gaagccgagg tccggatcaa aggccgagac gatgcggttc
     3001 tggaagttgt tcgaccacag cgccgtggag atgatcatgg tcggcgaccg gtagcggtag
     3061 ccaacgtccc acgcctggct ggtttcaggc tccacgttcg acagcacgat cggcgcatcc
     3121 gtcgaggtcg tcttcacgcc ggccgtgtac aggttgtcgg tccgcggcgc cgagaaacct
     3181 tcggcgtagc tgatgaacag cgtgtggccc ggcatgacct ggtaggacac gcccacgttc
     3241 ggcaggacgg cgtcgtagct gacgtcgcgc gagaacggca cgatgtagcg ggtgcccgtg
     3301 ccggcggcgt tgagggtgac caggttgtcg ctcggctgga cgccggcggt ggtgggatcg
     3361 ccgtcgacgt tggcgaacgt cggcgcgctg gtgcagagca ccgagaagcc gttgttctgc
     3421 gtcaggcagt actggttcag ctcacgcttg aacgtcggag cccgcacgcc gatcgtgacg
     3481 aacaggttgt cgtccatgaa acgaccgcgc cagtcggcgg agatctggtt caggatggcg
     3541 taggacaggc ggtcgcggct gcgcagggcg tagccgtcgg cgttgtagac cttctggccg
     3601 tagccgtcct tgccgccgaa cacgtcctgc gggtcgccgt tcccgttgat caggccgtac
     3661 tggccggtct ggcggtgacg gccgcggtcg taggtgtagg cgatgcggaa gctgttgttc
     3721 tcgttcggct cccagataag cgagcccatc gaggtgtagc gacgggtgtt ggtcgtgctg
     3781 ggcgaataga agcgcacgaa ggtgtcgcgg cagtcgccgt cgccgttgat gtcgaagccc
     3841 gagccgccgg tgccgccggg gccgcagagc acgccgccgg tcgagccggt gaccaccgtg
     3901 ttggcgccga ccgcggccac ttcgctgacc gtcgtgctgc cgccgccgtt ggccaggacg
     3961 tactggaacg ccgagtcgac ggtcagggtc agggtcgggg tcagggtgaa cctggcctgc
     4021 tcgcggatgt tgccggtgtt cgacgggttg atccgcaggc cgtagaagtt ggagttgtcg
     4081 gcgggatcgg cgtcgacgtc gccggtgccc ggcagggcca cgggggtcga gacgatcggc
     4141 aggtaggtcg ggttgttgtc gtagtccatg ccgttggcgc ggaaggtcgc catcgacgcg
     4201 gtgcgataga agtcgttgcg gttctcgttg tagtgaaccg ccagcgagat gaagtcgccg
     4261 ttgtcgccga tcggctggta gaccttggcg ttgacctgcc acttctgcag ctcgccggga
     4321 cccttgaact tgtcatactg ctgggtcgag gcggcgaaat aggcggtggt gccccagggg
     4381 ccgatctcgc cggtgtcgac catgccgaac aggcggcgat agttgaactc gccgacggcg
     4441 ccgttgagca cgaagcccat gtcggcgctc ggacggcggc tggtgtagtt gatcgtgccg
     4501 ccggtggccg acgcggtcgg gctgtccacg tcggtggtgc ccatgttgac gctggccttt
     4561 gagaccagtt ccggatcgag ctgctggttg gtgtagatgg cgtagttgcc ggtgtcgttc
     4621 agcgggatgc cgtcgaacgt cagcgagatg cgcgggccgt cgaagccgcg gatgcgcagg
     4681 ttgccgcccg acgaaccgta ggggtcgttg ttggtgaagt tcaggccggg cgtcaggttc
     4741 agcgtctgga tgatcgtctg accggtcggc tgggtggcga tgaactcctg gtcgatcgtg
     4801 gccttcgcct tgggcgcggt ttcaaccgag atcacgccat cgatgttcgg ctggccggcg
     4861 gtcgcggtga ccacgacttc ctcgacctcg gtggtgccgg tcgattgcgc aagggcgttc
     4921 gaggccagca gcatgctgcc gacgagcgcc gacgtcgcca caaaggcgag cttgttcttc
     4981 atcatagtgt gagtatcccc tctcatcccg acgccggctt ctaacctgga aacgacccgg
     5041 cccgggcaac tgtcacatgc cataacgccc caatgcgaag ctatggtgac tgccgtccga
     5101 acggtcctga acgcctgacc gtcatcctag ggcggagcgc cgatgaagca aggcgccgtt
     5161 aaggatcgag gcgattttgg gtccgtttgt cgcaagattg cgacaccttt ggggaaggtt
     5221 gtgcctttac agctcgccgc acgcgggcca gcgtccggcc cgcccatgac cccgcccgac
     5281 accgcgccgc cgtccgccga ccctcgccag cccctgcgca tggcgctggc gctggtcctt
     5341 gtgctgacag tcctgcggct ggccgcgatc ttcgccagcc cgcttgagct gaaccccgag
     5401 gaagcccagt actggctatg gtcgcggacg ctggacttcg gctatttctc caagccgccg
     5461 atgatcgcct gggcgatctg ggcgaccacc gcgatcggtg gcgacagcga ggcctgggtg
     5521 cggttttccg ccccgctgtt ccatgccgtg gcggggctgt cgttgttcgc cgccggccgc
     5581 cggctctatg acgactggac gggcctggcc gccgccgccc tctatctgct gatgccgggg
     5641 gtggcgctgt cgtccttcat cctggccacc gacgcgcccc tgctctgctt cctggccctc
     5701 gccctgctgg cctacgtgac cgtccgccag ggcgatcacc ggctggcccc ggccgccggc
     5761 ctcggcgcgg cccttggcct ggccatgctg tcgaagtacg cggccctcta cgccctgatc
     5821 ggcatcggcc tgcacctggc cctgtcgaag gacgcccggc gggcctggga tctgaggacc
     5881 gcggccgtgg ccctggtcgc cttcgccgcc gtgctggcgc cgaacctggt gtggaacgcc
     5941 aatcaccact tcgccacggt cggccacacc gcggccaacg ccaacctcgg cggcaagctg
     6001 ttcaatccgg gcgagatgct gggcttcctc ggcgggcagt tcggcgtgtt cgggccggtc
     6061 ccgttcgggg tgctggccgg cggggcggcg gtgctggcct ggcgccgccg gctgcagccg
     6121 caggatctgc tgctgttgtg cttcgccgtg ccgccgctgg ctgtggtcac cctcggcgcc
     6181 ctgctgtcgc gggccaatga caactgggcg gccagcgcct atgtcgccgg cgccgtgctg
     6241 gcggccgccc tgctggtgcg ctggcgggcc agatggnnnn nnnnnnnnnn nnncggcggg
     6301 cttggcctgc aggcggccct ggtggtgttc atgctgctgg ccgtggccaa tccggcgatc
     6361 gtcgacgccc tgggccggtc caatgacatc aagcgcgcgc gtggctggaa gcagatggcg
     6421 cagcaggtgg tccagcgggc ccaggtcgag cagatcaacg gcgggctgtc ggccatcgcg
     6481 gtcgatgacc gcttcctgtt caacgccctg gcctactacg gccgcgacta catcggcacg
     6541 ccggacgctc cgccgctggc catctggctg aggcgcagcc atgcgggcaa ccaggccgaa
     6601 accgtcgccc cgctgacgcc ggcgctcggg cggcgcgtgc tggtggtcag tctcgagggc
     6661 ctgcagggcc agcggatcgc cgccgatttc gccacgtccc ggaccgtcgg cgtgctgcgc
     6721 cagatcctca gcgtcaaatg cgcggcccgc tatcgcgacg acgagcggaa gttcaaccac
     6781 cgctgcctgc gccgcgagac cctgattctc ggcgagggct tcgccccggt gaagaaccgg
     6841 gactgagcgg ggacatgcac ttcagtgcgc gtcccctgcc aatcgacaaa acgggacacg
     6901 cactgaagtg catgtcccgg cgaactcaga ccgcgacctc gaacgccgct tcggtcttgc
     6961 ccttcacctc gtcaaccgtc acgcccggcg ccagttcgat cagccgcacc ggcgtccggc
     7021 cgcggttgat ctcgaacacg cccagctcgg tgatcagcag gtcgaccacc cccgcgccgg
     7081 tcagaggcag gttgcagcgg tgcagcagct tgggcgcgcc ggtcttctcg caatggtcca
     7141 tgaccacgac cacccgcttg acgccggcga cgaggtccat ggccccgccc atgcccttga
     7201 ccatcttgcc gggcaccatc cagttggcca ggtcgccgtt ctcggccacc tgcatgccgc
     7261 ccaggatcga cagggcgatg tggccgccgc ggatcatggc gaagctgtcg gcggaagaga
     7321 aatacgagct cgaatccagc tcggtgatgg tctgcttgcc ggcgttgatc aggtcggcgt
     7381 cctcttcccc ctcgtagggg aacgggccca tgccgagcat gccgttctcg ctctgcagcg
     7441 tcacatgcat cccctcggga atgtagttgg ccaccagcgt cgggatgccg atgccgagat
     7501 tgacgtagaa gccgtcacgc agctcgcggg ccgcgcgggc ggccatgtct tcgcgggtcc
     7561 aggccatcag gcgacttcct tcacgcgggt ggtcacgcgc tcgatgcgct tttcgaacac
     7621 ggcgcccctg aacaggcgat cgacgtagat gcccggggtg tggatgttgt ccttgtcgag
     7681 gtcgcctatg gcgaccagct gctcgacctc gacgaccgtc gccttgccgg cggtggccat
     7741 cattggattg aagtttcgcg cggtctttcg gtagacgagg ttgccctcgg cgtcgccctt
     7801 ccaggccttg acgatcgaca ggtcggcggt caggccgcgc tccatcacat agcgctcgcc
     7861 gtcgaactcg cggacttcct tgccctcggc gaccagggtg ccgacgccgg tcctggtgaa
     7921 gaaggccggg atgccggcgc cgccggcgcg gatgcgctcg gcgagcgtgc cctgcggatt
     7981 gaactccagc tcaaggtcgc cggagaggta aagctgttcg aacagcttgt tctcccccac
     8041 ataggagctg atcatctttc tgatctgccg gttctcgagc aggatgccca ggccgaaccc
     8101 gtcgacgccg cagttgttgg agacgaccgt gaggtccttg accccgcttt cgcggatggc
     8161 cgcgatcagg ttctcgggaa tgccgcacag gccgaagcct ccggccatga tcgtcatgcc
     8221 gtcgaaaagc agccccgaca gggcctcgac ggcgtccttg gcaaccttgt ccgccatgat
     8281 cgctccgttc gaaaaggtcg gcccctctcg cccgatccgg caagaaattc aagccttggt
     8341 gaataatttg gatgccttcg gggaacgcta tcatggaggc tgaaggccgc gaaccctcgg
     8401 tgctgctggt gttcgcccat ccggcgctgg agcggtcgcg ggccaacgcc gtgctggcgg
     8461 gagccgggcg ttcggccgct ggcgtccggt tccacgacct gtacgaggtc tatccggatt
     8521 tcgtcatcga tgtcgaagcc gagcagcaaa ggctcttggc ccaccaggtc atcgccctgc
     8581 agtttccgtt ctactggtat tcgaccccgg ccctgatgaa ggagtggctg gatctggtct
     8641 ggctgcatgg cttcgcctac ggcaagggcg gcgaccggct gaagggcaag cagctgttcg
     8701 tcgcctgcac caccggtggc ccggcccagg cctatcacca gcgcggctac aaccggttca
     8761 ccatggacga gttcctgcgg ccgcttgagc agacggccgc cctgtgcggc atgacctggg
     8821 aaacgccgtt cgtggtccat ggcgcccgcc tcaaggacga ggcccagatc cgcggtgagg
     8881 ccgatcgcta ccggtcgcgg ctggagggcc tggccgccgc cgcgcgggag accgtctgat
     8941 gcatggcgac gtgctgaccc aggccctggt ctatctcgcc gccggcgtgg tcaccgtcgc
     9001 ggcggccaag aagctcggtc tcggttcggt gctgggctac ctgatcgccg gcgcggtgat
     9061 cggccccttc gccctgaacc tgaccggcga ccccgaggac gtcatgcgct tcgccgaatt
     9121 cggcgtggtg atcctgctgt tcctgatcgg cctggaagtg cggccggccc tgctgtggtc
     9181 gatgcgcaag accatcttcg gcctgggggg cgcccaggtg gcggcgaccg ccctggccat
     9241 cgccggggcc ggcatgatcc ttggccttcc ctggccgacg gccctggccg cgggcctggt
     9301 gctggccatg tcctccacgg ccatcgtgct gcagaccctc gacgagaagg gtctgcgcca
     9361 ggggccggtc ggccgggccg cgttcggggt gctgctgctg caggacctgt cgatcatccc
     9421 cttgttcgcg ctgctgccgc tgctggccac cggaccggtg accctgacgg cggacaccgc
     9481 ccattcgacg agtctggtgg ccgacctgcc cgcctggatg caggcgctgg ccgtgctggc
     9541 cgcggtcggg gcggtggtcg ggggcggccg gttcgtcgtc cggccggtgt tccgcttcat
     9601 cgcccaggcc cgcctgcggg agatcttcac ggcctcggcc ctgctgatcg tggtcggggt
     9661 cgccgcgctg atgcagatcg tcggtctgtc gccggcgctc ggcgccttcc tggccggggt
     9721 ggtgctggcc gagagcgagt tccgccgcga gcttgagacc gacatcgagc cgttccgcgg
     9781 cctgctgctg ggcctgttct tcatcaccgt cggcgcgacc atcaacttcg ccctgatcct
     9841 gcgcgagccg ttgctgctga tcggcctggt gctggggctg atggcgctga agttcgccgt
     9901 gatgttcgcc ctggcgcggg tcggcggggc gtcgaaccgg ctggcggcca cggtggccgt
     9961 ggccctggcc cagggcggcg aattcgcctt cgtgctgctg accttcacgg tcggcgcggg
    10021 ggtcatcaac caggccctgg ccgacctgct gaccgcggtg gtggccctgt cgatggccat
    10081 gacccccctg accgtggtcg cctacgagaa gatcctgacc cggctggacc ggggcaggcc
    10141 gggcgaaacg atcgccgatc cgaccttcga ggacgccgac gccgagccgg acatcatcat
    10201 cgccggcttc ggccgcttcg gccaggtcac gggccggctg ctgacggcca acggcttcaa
    10261 gtcgaccgtg ctggacgccg atatcgagca gatcgacctg ctgcgccgct ttggccggcg
    10321 ggtgcactac ggcgacgcga cgcggctgga cctgctgcgc caggccggcg ccgagcgggc
    10381 gcggatgctg atcgtggcca tcgacgacca ggaaaaggcc gccgaactgg tcgagcgggc
    10441 gcgcgaggct ttccccgacc tgaccatcct ggcccgggcg ttcgaccgcc gccacgccta
    10501 cgacctgctg gccagcggcg ccgacgcggt cgagcgggaa accttcgagg gcgccctggc
    10561 gatgggcgtg acggcgctga aggcgctggg cttccgcgcc catcgcgccc agcgggcggc
    10621 cgactacttc cgccgccacg accggcgaac gttcgacgag ctccggccga tgtggggcca
    10681 gaccgaagcc tatctgctgg cctcgcgcga cgcggcccag accatggaca ggctgctgca
    10741 ggcggacctg gcgaagatga agcccggcgc ggccgacggc tgggacacca gcggccgcga
    10801 cgaggaacag cgcgagacgg ccatgggcgg cgaggactga cctggatcat tgtcgcgtcg
    10861 tcacgacgcc acgagaggtg taaacagccg cgtttcgatg tccgggagac cccgcttgcc
    10921 ccagtccgcc atcgcccccg tgtccttcgc cggctacgcc gccgacttcg acgccttcgc
    10981 cgaggcgctc ggggccagtt tccgccgcta cggcttcgcc gtgctgtccg actacgacct
    11041 ggaccaggcc cggatcgacg cggccatcga cgcggccaag cgcttcttcg ccctgcccga
    11101 ggacgtgaag aagcagtacg ccgtcggcaa gggcggtcag cgcggctaca tcccgttcgg
    11161 catcgagacg gccaagggcg ccgatcattt cgacctcaag gagttctggc acatgggccg
    11221 cgacctgccg cccggccatc gcttcgaggc ccagatgccg cccaacgtct ggccggccga
    11281 ggtggcggac ttccacgaga aggttgcctg gctgtacaac gccctcgacg ccatgggcgt
    11341 gaaggtcctg caagccatcg cccgctacct tggcctgccg cgcgaccagt tcgacgccgc
    11401 cgtggagaac ggcaacagca tcctgcgcct gctgcactat ccgccgatcc cggccgacgc
    11461 gaccggcgtg cgggccgggg cccacggcga catcaacgcc atcaccctgc tgctgggcgc
    11521 cgaggagggc ggactggagg tgctcgaccg cgacgggcgc tggctgccga tcaatccccc
    11581 acccggctgc ctggtcatca acatcggcga catgctcgag cgggcgacca acgacgtcct
    11641 gccctcgacc gtccaccggg tggtcaaccc gccacccgag cgccgggcct tcccgcgcta
    11701 ctccacgccg ttcttcctgc atttcgcgcc ggactacgag atccgcaccc ttcccggctg
    11761 cgccacggcg gacaatccgg accgctaccc cgagccgatc accgccgacg ccttcctcgt
    11821 ccagcggttg aaggagatca agctgctcta ggcggcggcg gagtcacccc gaattaaccc
    11881 tgcgtgctca cggtatcgac aaacaggggc gggggaatcg ggcatggcgc cttcggacgc
    11941 cgcaaggctg acggaaatcc ggtcgctgga cctcgacgcc ttgcgcgggg tcatcgaccg
    12001 ggtcacgcgg ctggcccgcg gcgcctcggg cgcggttcac gcctatgtcg ttctggtcga
    12061 ggaggaccac gtctggcatt ccggcttcga cggcatgccc gagcacatcg gcccgtcgtc
    12121 ggactcgacc accgcccgcc acatcgccga tccggacacc ctgtggtccg aggacctcgc
    12181 cagggactgg cccgaccacc cgtgggtctc cggcccgccg cacgcgcgcg gctacgccaa
    12241 cgccccgatc atcctgccgg gcggcctcag gctcggcgga ctgtgcttga tcttcggcga
    12301 accgaggccc cgcgatgcgg gggtggtcgc cacgctcaac gatctggccg cccttctggc
    12361 cgaggcgatc gaacgactgc gggcccagcg ccgcaccgag gcggccgaga ccatctcccg
    12421 cgcggcgaca gacctgaagg acgcgatcat gcgctcctcg ccggtcgccc tgggcatgac
    12481 cgaccgcgac ctgcgctacc tctatgtcaa cgagcagtgg gagctcgaga agggcatcac
    12541 ctcggccgaa gcgctcggcc ggaccatggc cgaactgttt ccccggtcct atccgctgct
    12601 ggccgggtcc tacgaggagt gcctggcggg caataccgtc tcggccgagc ggattctcgt
    12661 gcccagcggt cgccggccgg accgctggct gcgggcgacc ctctgtccct ggtacgaggc
    12721 cggcggcgag gtcgggggcc tgatctcgat gagccacgac gtcagcgacg tcatcggttc
    12781 gctgaagatg gccgaacgcg cccgcaagcg cctggcgctg gccctcgaga tcgccgaagt
    12841 gctggtctac gaggtcgact accgggcgaa gaccctcaag gtcgacggcg ccagcgacac
    12901 cttcttcggc ggcgccctga cctatgacga cgtggcccgc gacatgtggg tgacggtcca
    12961 ccccgaagac cgcgccgcgg cccaggccat ctgggaagac cacctggcga cgggcaggcc
    13021 gttccgcacc gaataccgga tgaatccgcc cgacggccgc gaggtctggg ccttctcggc
    13081 ggccgagctc ctgcgcgacg acgacggccg gatcaccggc gtgctcgggg tgctcaagaa
    13141 catcaccagc cgcaagcatg tcgaggaagc caccgttcgg gcgcgcgacc tggccgaggc
    13201 ggccaaccgg gccaagagcg agttcctcgc caacatgagc cacgagatcc gcacgcccct
    13261 gaacggcgtg atgggcgtgg ccgcggccct gtcgcgcacc gacctgaccc cggcccaggc
    13321 cgagatggtc gggctgatcg agacgtccgg ccagactctc gaggcgatcc tcgccgacgt
    13381 gctggacctg gcgcgcatcg aatccgggcg gctggagctg aaaccggagc cgttcgatct
    13441 cgacgcctgc ctgcgatccg cggcggccct gttccagccc ggcgccgacg ccaagggcct
    13501 ggagttcgat ctggccatcg acgcagcggc ctcggggacc ttcatcggcg acgcggtgcg
    13561 gctgcggcag gtggtctgca acctgctgtc caacgcggtc aagttcacca gccggggcgc
    13621 catcggcctc tcggcgacgg tcggggccga cggcggccta gccatcacgg tcagcgacac
    13681 cggcataggc ttcgacgccg acgtcaagga gcggctgttc gagcgtttcg agcaggccga
    13741 cggctcgatc acccgccggt tcggcggcac cggtctgggc ctggccatct cgcgcgcgct
    13801 ggccgaggcc atgggcggaa cactcgacgc ccggtccgag cccggcgccg gatcgagctt
    13861 cgtcctgacc ctcgccctgg aacgaagcct cgaacccctg acagccccgg cccgtccgga
    13921 ccagccggcc ctggccgaca cccgcgcgcc gcgcgtgctg ctggccgagg accacgccat
    13981 caaccgcaag gtcgtcgaac tgctgttcgg tggcgccggc gtcgagctga ccagcgtcga
    14041 gaacggcgcc gaagccgtcg cggcggtcgc cggcgccgac ttcgacctgg tcctgatgga
    14101 catgcagatg ccggtgatgg acggcctgac cgcgatccgg gcgatccgcg ccgccgaggc
    14161 cgcgcaaggc cgcccgccga cgccgatctg ggcgttgtcg gccaatgccc tgcccgagca
    14221 cgtggcggcc tccctggccg ccggcgccga tggtcacctg agcaagccgg tctcggccag
    14281 cgcactgttc gacgcgctcg agaacgcctg cgcgcgcgga cagcccgaag ccatcgccct
    14341 caacgtttga atcgcgaccc tggccggaag ccggacggcc cgacgcgcgc cggcctcgcc
    14401 gctacctgga cagcctcagc ttggcgatat cccggccgat ggcggcgaag gcgtccttga
    14461 gcgcctcgcc gctcgacggc aggtagaagt gctcggcgtc ggtggcgcag tcggacatca
    14521 gttcggcggc caggctcgcg gcccgcacgt cgaagccgac ggtatagatg atcacgccct
    14581 tggccttcat gttggcgcac agccgcgagg cctggtcata ggcgtcgccg ttggtggcgt
    14641 cgcagttgat cttgtccgag gtgcccccgc tgcccggccc cgcgttccgg gcgatcacgc
    14701 cgttgcagta ggaggtgttg aagtcgccgt cggtcatcag cacgacgacc ttcagcagct
    14761 ccggggtgcc gtaggccgcc gggcggctgg ccgccggcca gagatagccg aagtcgggcg
    14821 cgaccgagta ccagccccag gccaggccga tctggccggc cgtcgaaccg ccgtcgtcat
    14881 agctgtcgat ctgcgccttg agcatggtct tgtcggtcga caggggcgtc aggacgctgt
    14941 cggggcaggg attgttggag ctcgggtagt tgcggcccac cggcgcgccg cccggcgaca
    15001 cgtcggtata ggcggcgagg cccgtgcgct cggtcacgca ggtgctgacc tcgaacatct
    15061 tcggctgccc cgcggccgag gtgaaggcgt aatatcggca gccgggggcg gtgcagtcgg
    15121 ccttgccgcc cgaggtgtag gttccgtagt cggccccgtt cacgcccgac agcgagaagg
    15181 tgcttcccgt gctgttggcg acggtgaagg tctggtcatt cagctcggtc atgccggaga
    15241 cgccggagat gtagacatcc tcgccgtcgg tgaggtcgtg acccgccgcc gtgacgacga
    15301 tcgaacagtc cgacaccctg catttgcgga tcgtcccgcc cgacttgtac ttgctgtagc
    15361 tagagctctt cacgccctgc agctggaagg aattggtcgt gcggttggcg accacgaagg
    15421 cgcggtcgtt gagctcggtc atgcccttga cgccggtgat ccagacgacg tcgccgttgc
    15481 tgaagccgtg gccgttggac gtcaccaccg cggggttggc cttggtgacg ccggaaatcg
    15541 accgcgacga acccgtcgtc caggaggcgt cggtgatggt ccagggaccc cggatggcgc
    15601 cgcgcgcgcc cgaggcgtaa ccgccgagat tcacggcggc cgaatagggc accagcgcga
    15661 ccttcgaata gtagggcgtc tggacatcct gcacgaccat gtcgaccagg tccttggcgg
    15721 ccgaccgcac gtcacggatc ttctggccgg ccatcgagcc ggtgatgtcg agcaccaggg
    15781 cgacttcgag attggtcgag gcgcgcacca cctcggcctc ggccccgatg gtcatgtccc
    15841 cctgcagcca caggttggcg atcacgggcg tcaccttggc cgtcgccgtg ccgaccacct
    15901 tcgtaccgac gaccttgaag cttgagctgg acagggtggc gtcgctgacg ccgctgagct
    15961 gggccgccag ggcggcggcg ccgatcggct ggatcgtctc gtccgtattg gccgaagagc
    16021 gcgccgccat cagggtcgcc gcgtcgaggg cgtcctgcag gtgccgcttt tcaacgctgg
    16081 cccggctgac atcgatcagg ccgaagccga gcaccgccgt cggcaacgcc agcacggcga
    16141 accagatggc caccgcgccg cgctcgctgc ggaagcggcg caggaaacgg acagcgggct
    16201 cagtgagccc cgaggcgagc actttgaagg cgcgttgcat ggattttcat ccccatcgac
    16261 gggtcatctt cgcgccacag ggtaaggaaa ccgccaacac aggtggtgaa tccggcttta
    16321 ccgcgccgcc tatcgcgaga tgcgcagccg ggagatatcc tcgccgatgg cgtggaaggc
    16381 gtccttcagc gccgcgccgg tcgacggcag gtagacatgc tcggcgtccg tggcgcactg
    16441 ctggacgatg ctctgggcgg ccgcgtcgga ggccacgtcg aagcccaccg tgtagatgat
    16501 cactccggcg gccttcatgt tggcgcacag ggtctgggcc tggctgaagg ccccgccgtt
    16561 gggcgcgttg cagttgacat ggtccgagga cgagccgctg cccgagccgg aatcccggct
    16621 gatgacgccg ttgcagtagg tggtgttgaa ggccccgtcg gtcatcagca cgaccacctt
    16681 gagcagcttg tcggcgccat aggcggccgg ctggctggcg gtcggccaca gcgaggcgaa
    16741 gttgggcgag accaggtacc agccccaggc cagacccagg tggcccgccg tcgatccggc
    16801 cgcggccagg ccgtcgatgc ggctcttcag cgccgacttg tcggtcgaca gcggcatgat
    16861 ggtgctggcc aggcagggat tgccgctggc cgggtagttg cgccccagga aggtcgacga
    16921 cggcgcggcg tcggtgaagg cgttggtccc ggtccgttcg gtgacgcagg tgctgatgtt
    16981 gaacgtcctg gtcccgcccg aggcgttggt gaacttgtag ctggcgcagc cgggcgtggt
    17041 gcaggttccc gccgtgtagg cgccccggac gctgtcggcg taggttccga cgttgacggc
    17101 ggccgagtag ggcgccagcg ccagcttggt gtagtagggg ctctgctggt cctgcacgac
    17161 cagatcgacg agatccttgg ccgccgcctt gaggtcggcg atcttctgtc cgctcatcga
    17221 accggtgacg tccagcgcca gcgccacctc gatgttgttg gacgcccggg tgacctccga
    17281 ctccgcgccg atggtcatgt cgccgtcgcg ccagaggttg gccaccaccg tggtcatcga
    17341 cgcccgggcc gtggccacca ccttcgaccc gtcgaggcgg aagctggatg accgcagcac
    17401 cgcctccgtc gcggtcagat ccgcggccag gacggcctcg ccgaccgcct gaagttcggc
    17461 gtcggtggtg gcgggggacc gcgcggcggc cagggtcgcc gcatcgagcg cgtcctgcag
    17521 tcgcatcttc gcggccgagc cgcggctgac gtcgagcgcc ccgaagacca tcaccgccat
    17581 cggcagcagg accatggcga actgcacggc caccgcgccg tcctcgcgcc gccaggcgct
    17641 cagcagccgg cccagccgct ccggactcag gaatcccgtc ttcatcgccc tgccccgaca
    17701 gagttgcgaa caacgggcga tcctggcgcg ggcgcggtaa acgcatgggc cacaagacaa
    17761 ggtcaaagtc gggaaaccat gtcggcctat cgacggcgtc ggaatcccgt gggagtctgc
    17821 cccacccggc gtgagaacag ggcgcgatgg acactctgca actcctgctg cgaggcctgg
    17881 ccgtcggcgc ggtcctgacc accgggatcg gcctcctgcg cagcacccat gccggacccg
    17941 cgcgctgggt cggggccgtc ttctgcctct cggtcgcggc cttcggcgtc cattcgggcg
    18001 gcgcggaaac gcgcgccctg catcttctgg gagggccgat ctggctgctg tccgtcacgg
    18061 cgaccgcctg gttctggctg ttcgccgtga cgctgttcga ggaccgcggc gtcaccctgg
    18121 agcggctggc ccccgtcggg atcatgacgg cggtcgcgac cgtcggcgcg gtgctgcccc
    18181 ggccggcgtc cgatggcgca tggattgtcc ataacctgtt gcaggtcgtg ctggtgtcgc
    18241 acgtcgtgcg gatcatttgg ctcagctggg gtggcgacct ggtcgaggcg cggcgatccc
    18301 tgcgcggccc gttcatggtc gcggtcgcgg tctactgcat cttcctctcc ggcttcgaga
    18361 tcgccggcac cctcggcttt catctgggat ggggatcact ggtccaggcg ctaagtctcg
    18421 cgatcatgac gctggccggc gcggcgaccc tgctgcaggg ccggcccgac ctgttcgagt
    18481 cggcggttcg tcgccccgcc gccgcggccg aggccgtggc cccgcaggac cgccccgacc
    18541 tcgcccggct gcagacgatc atggccgata ccgacctgtg gcggcgcgag ggcctgacca
    18601 tcggccagct ggcgtccgaa ctgggcgttc ccgagcaccg gctgcgtcgg ctgatcaacg
    18661 gcgggctcgg ccatcgcaac ttcgccgact ttctcaatgc ccgccgcatc gacgcggcga
    18721 aggcggccct ggccgcgccg gacaacgccc gcgcctcgat ctccgccctg gccttcgagc
    18781 tcggctacgg ttccctgggg ccgttcaacc gggcgttcaa ggacgccacc ggcctgacgc
    18841 cgtccgcctg gcggctcagg gccctggccg cctcgccgaa tccggaaaag accggctgat
    18901 tccggaatcc gccagccccg gaagacctgg ggaagcggcc aggcccggcg gctgtcaccg
    18961 tggagtcatc gaccttccgg agtgacttca tgacccagac gggaattcag ctactggacc
    19021 tcggccgcca ctggctggcg ggcttccaga acgacatggt ccgctacatc gtcgccgcgc
    19081 ccctgacctg gctggtgctg tgcgtgatct gcgcgccgct gctgcgccat cgcaagattc
    19141 gccccgacag cccgcccgcg cgccagctgg tcgtcgagtt cctctgctcg gtccgctcgg
    19201 tggcggtgtt ctcgaccgtc tccctgctca gcgtgctgat gtggaaggcc gggctgctgt
    19261 ggggcgacga gctgcgcgaa catgccggtc cggtgtggat cgtggtctcg ttcgccgtca
    19321 cggtgatcgc ccacgacgcc tatttctact gggcccaccg gatcatgcac gaccctcgcc
    19381 tgttccggcg cttccaccgc aggcatcatc gctcgcacaa tcccacgccg ttcacggcct
    19441 acagtttcga cctggcggag gccgcggtga tggccagctt cacggtgatc tgggaactgg
    19501 tcttcccggc gtcctggatc ggcttcaccg ccttcctgat ccaccagatc gcccgcaacg
    19561 tgatcggtca ctccggctac gagctgttcc cggccaaccg ccggggccgg ccgctgctgg
    19621 actggatgac cacggtcacc catcacgacc tgcaccacgc ccaggccggc tggaactacg
    19681 gcctctactt cacctggtgg gaccgctgga tgggcaccga gcacccgcag taccacgcgc
    19741 gcttcgcggc ggtgacggcg ccccgccggg cggcggcgct ggcggcggcc gaatagaaaa
    19801 gggggaggcg gatcgctccg cctcccccgt tccgtccggt cggaccgcga cctaccagcc
    19861 gcagtgctcg cccttgttct gcttggtcag ccagatgttc agcggcttga agtagtcggc
    19921 caccgccgag gcgtcgccct gggtctcgcc ggtaaaggcc gccatcgcct ccggccaggg
    19981 gcgcgaggcg cccatctcga gcatggtgtt gaacttcgcc ccgaccgcct tgttgccata
    20041 gatcgagcag cgatgcagcg gtccggtcca gccggcctgc cggcaggccg cgcgctggaa
    20101 ctggaactgg tagacgtggg ccaggaagta gcgcgtgtag ggcacgttgc ccggcacgtg
    20161 atacttggcc cccggatcga aggcgtcggc cgggcgcggc cccggcggga cgatgccctg
    20221 gtactgggtg cgcagcttcc accagccggc gttgtactgg tcgggcgtca gctggccgga
    20281 gaagacctcc cagcgccagc ggtcgaccag caggccgaaa ggcatgaagg cgatcttgtc
    20341 gagggccatc ttcagcagga aggggatgtc gccctcctcg cccggcacgt ccttgagcag
    20401 gccgatctgc tgcagatagg tcggcgtcag ggccgacagg ccgacgaagt cgccgatcgc
    20461 ctcgtggaag ccgtcgtttg ccccgccctt gaagatgtag ggctggttct tgtaggcccg
    20521 ctggtagaag ttgtgcccca gctcgtggtg gacggtatag aagtcctccg cgttgacctt
    20581 ggtgcacatc ttgatgcgga tgtcgtcggc gtcgtcgagg tcccaggccg aggcgtggca
    20641 gaccacctcg cggtcgcgcg gccgggtgat ctgcgagcgg gtccagaagg tctccggcag
    20701 cggggccaga cccatcgaga cgtagaaggc ctcgccggtc ttgaccattt tcaccggatc
    20761 ataccccgcg ttcaccagca gctcgtcgag gctgtaggac ggcgtggtgc tcttgggcgc
    20821 gacgatcgga tagatgttgc cccactgctg ggcccacata ttgcccagca ggtcggcgcg
    20881 gatcgggccg gtgcgcggct ggaccgcatc gccgtatttc tcgttcagcc gggctcgcac
    20941 atagcagtgc agattgcggt agaacggctc cagctgcttc cacagccggt cggtctcggc
    21001 ggcgaaggcg tccggatcca tgtcgtagcc ggaccgccac agggcgccgg tgtccttgta
    21061 gccgagcgcc accgacccct cgttggagag cgccacgagc cgggcgtagt cgggacgcat
    21121 gaccgtcgag atcgaatgcc agccttccca gaccgccttc agctcggccg gatcggtgct
    21181 cgaggcgagg atgtcctcgg cgtcgttcag ggtcagggtc ttgccgttgt aggtgaattt
    21241 gccggtcgag taggtcgagt ccagccgtga ggcgatggtc gccatctcgt ccgccgcgcc
    21301 gggccgctgg ggcgccggcg ccaccagggc gcgcttgagg atgtcgagct tgcggcgggt
    21361 caccgggtcg accgcgacct tgtcgtagcg cgcggcctcg atcgccaggc gggtcgccag
    21421 gtcggtgatc tcggcgccgg ccttggcctc g
//
LOCUS       DAORNX010000005        19942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2104_length_19942_cov_7.549153, whole genome shotgun sequence.
ACCESSION   DAORNX010000005 DAORNX010000000
VERSION     DAORNX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 19942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19942
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_19942_cov_7.549153"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLO65_00465"
     CDS             complement(<1..1048)
                     /locus_tag="PLO65_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRD26751.1"
                     /translation="MRAPLLIIASFLALPLVAAEAAERLAGDTPVVALGRGAGDRLTL
                     PAPKPGRVRPLVVVVGENQGAEVTDFIIPYGVLKDSGVADVRSLSTGEGPVRLRMALT
                     IRADQTLAQFDQAEPAGADIVIVPAQVEPRDRRLTVWVKRQADRGATIVAICEGARVV
                     ANAGLFHGRHATTHWHALKALEKAYPATTWVRDRRYVQDGPVISTTGVTASIPVSLAL
                     VEAIGGSAAARATARRLGVEDWSAVHRTADFHLSRTDYARAIAALAAVWSHETVEAPV
                     AEGFDEVALALRTDVWGRSFRARVVTTRAGRSPVRSRHSLTLLPDAEPEAGRFQIPGG
                     AGPAASQLDQALAAM"
     gene            1337..2326
                     /locus_tag="PLO65_00470"
     CDS             1337..2326
                     /locus_tag="PLO65_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRD26752.1"
                     /translation="MTLVDTRPRNIALVGYAGAQSLDLVGPLEVFSMANNYGGGDRYR
                     PILASFEGGDIRCNSGLILAGATALADLPEDLDTILVAGGSEAGLRGQENGPLLDWLV
                     ARRATTRRVGSVCSGALVLAATGLLDGRRATTHWGVCDVLQARHPEIEVDADAIFVAD
                     PPFFTSAGVTAGIDLCLALVEADCGPDIALAVARNLVLFMRRPGGQTQYSAGLNVQAA
                     ASPRLRRLVVDITADPVGDLSLPSLAARAGMTERTFSRVFHRETGATPAAFVEAARLN
                     RAKALLESSDWPLARVAERAGFGSLDALHRAFQKRLGATPGEYRQRFGRPTTA"
     gene            complement(2341..3699)
                     /locus_tag="PLO65_00475"
     CDS             complement(2341..3699)
                     /locus_tag="PLO65_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26753.1"
                     /translation="MSARSGKADGGLAWPRRAVATAAVLTSMALVVLDASVANIALPT
                     IARSLRVAPPTAVLVMTAYQTALVIALLPCGALGERYGYRRMFTWGVTIFTLASALCA
                     LAPSLPWLVAARFLQGLGGAGVMALGVALLRFSVPRGRLGSAIGWNAMTVALSSAAGP
                     TIGALVLTGADWPWLFALNLPLGVVVLFASRALPPVIGRPQAIDLGSVLLNALLFASL
                     VVGAELLPDRPALAVGLFAVAALGLAALVQRESPKATPLFPLDLLRSGAFRVSVIASV
                     CCFAGQSAGLLALPFQLQAGLGQTPLTTGLYMTVWPLAVAGTAIVVSRLADRASMVWL
                     CAAGGAGLAAGLLALALWPLENDLRPLIPMTALCGVGFGLFQAANNRNLFLSAPPERS
                     GAAGGMQGTARLTGQTAGAVLVTLLFATTSVEATPTIAFGIGAALALAAGLVSLHHVR
                     VH"
     gene            complement(3717..4265)
                     /locus_tag="PLO65_00480"
     CDS             complement(3717..4265)
                     /locus_tag="PLO65_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26754.1"
                     /translation="MENTSLDTFVAAVRSAWGPLTSELVTGCSRALEAALKAPPAEPW
                     LAALHRDRPANAELHRDPALGFVLLAHTEQAGLYRPPHDHGRSWVVYAIQQGEIEMGS
                     YVRIENPDGEIRLVERDRTVLRAGQVKAYLPGDIHDTRCLSPSALLFRFTERDLKVED
                     LQEHRVTRYVRMEGGWSPGRPR"
     gene            4318..5316
                     /locus_tag="PLO65_00485"
     CDS             4318..5316
                     /locus_tag="PLO65_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD26755.1"
                     /translation="MAERAAFALYCCIKRYVTREPAIRPMTRPDLNLLVTLDALLTEG
                     SVARAARRLRLSPSAMSRALARLRETIGDPLLVRAGRGLVPTPRALALREQAHQLLQD
                     VDAVLRPEQTIDLARLTRTFTLRTSDGFVESFGPGLIARVGEDAPGVRLHFLQKPDKD
                     SGPLRDGRVDLETGVVESSLGPEVRTQALFRDRLIGVVRAEHPLGRGAVTTARYLAER
                     HVVVSRSGFDDDAVDQRFLPPALKRRVAATIVSGFSAALSLARLSDLVATVPDRHTAN
                     LRDGLHTFALPAPTLDFAVSMLWHPRMDSDPAHRWLRGCLREICAPAGPGLSTGRP"
     gene            5477..5893
                     /locus_tag="PLO65_00490"
     CDS             5477..5893
                     /locus_tag="PLO65_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRD26756.1"
                     /translation="MALATSKTLENLKAAFAGESQANRRYLYFAQKADVEGYNDVAAV
                     FRSTAEGETGHAHGHLEFMEAVGDPATGLPIGGTGDNLKAAIAGETHEYTDMYPGMAR
                     TAREEGFDEIADWFETLAKAEKSHAGRFQRALDTLD"
     gene            6006..7388
                     /locus_tag="PLO65_00495"
     CDS             6006..7388
                     /locus_tag="PLO65_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRD26757.1"
                     /translation="MTDEPTALPPAAARAEGSLDAPMRHPIAWRDDSYYDLAAVEKEM
                     ERVFDICHGCRRCFNLCDSFPRLFDMIDEGPTGELDGVPKEKYAEVAEACTLCDMCFL
                     TKCPYVPPHEFDLDFPHLILRYRAAKRRASGGEFVREQLGETDRNGKLARPVAGLANW
                     ATARENTPLRGLLEAIAGIDREAELPKFHSRTAKDRLKTMPAADPAGPAYGQRKAVIY
                     ATCFSNYNDPATAEAAAAVLAKQGVETAFCYPECCGMPQLEAGDLSDVAGRAERTAAA
                     FAPYIAQGYEVIALTASCGLMMKFEWPLMLPESAAVQALARATKDISQYVVGLSKAFG
                     LAPGLSPVPGGVTVHHACHARAQNMGAQSAQMLRLIPDTRIELVERCSGHGGTFGVMK
                     ATRPAAVKVGRPAARQVAQKKTEEICSDCPLACTHLGQMVAAETGGAQVHTGHPIEVL
                     AKAYGLAFQN"
     gene            7399..7998
                     /locus_tag="PLO65_00500"
     CDS             7399..7998
                     /locus_tag="PLO65_00500"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HRD26758.1"
                     /translation="MPASEHRITAADLIPDADYARQRAERRAALLPVKRLRRVALGPY
                     CTVIFENYDTMLFQVQEMLLTEKGGPAQVPDELAAYNPLIPQGSELVATVMFEIDEPI
                     RRAAALARLGGVEARFFLQFGADLRAGGVPDDDDIERTREDGKTSSVHFLRFPLDEAM
                     KAVFRDPTVTIAIGCDHEHYAHLAMLSPATRAELARDLS"
     gene            complement(8086..8196)
                     /locus_tag="PLO65_00505"
     CDS             complement(8086..8196)
                     /locus_tag="PLO65_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26759.1"
                     /translation="MAKGQKKSNKEVRKPKADKKPAAAPTSRFIEVAGKK"
     gene            8373..8567
                     /locus_tag="PLO65_00510"
     CDS             8373..8567
                     /locus_tag="PLO65_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26760.1"
                     /translation="MPDKSNIQGEGDYEAAKRFDDAERAFVETGPVKQKAREAEQALD
                     GPEGAELERARVESGKGDTG"
     gene            complement(8628..10493)
                     /locus_tag="PLO65_00515"
     CDS             complement(8628..10493)
                     /locus_tag="PLO65_00515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRD26761.1"
                     /translation="MGEAARQIPLGEAAGDTAWAGGEKPQLALVRKVEVDRSRDALLT
                     DFGKTTLEDRYLLAGESYQDMFARVATAFADDAEHAQRVYDYMSRLWFMPATPVLSNG
                     GADRGLPISCFLNAVGDSLDGIVGTWNENVWLAANGGGIGTYWGGVRSIGEKVKGAGQ
                     TSGIIPFIRVMDSLTLAISQGSLRRGSAAVYLDIHHPEIDEFLEIRKPSGDFNRKSLN
                     LHHGISITDEFMHAVRDGTKFGLRSPKTGEVLREVDARALWQKVLELRLQTGEPYLIF
                     ADHVNRAMPRHQRELGLQVRQSNLCSEIMLHTGKDHLGAERTAVCCLSSVNAETFLEW
                     RDEPRFIEDVMRFLDNVLEDFIKRAPQDAKHAIYAAQRERSVGLGLMGFHSFLQAQNV
                     PFESALAKSWNMRLFKHLRREADKASVKLAEEKGPCPDAAERGVMERFSHKLAIAPTA
                     SISIICGGTSAGIEPIPANIYTHKTLSGSFAVRNPYLERLLETKGANTPAVWDSILAN
                     EGSVQHLDCLSQDDKDVYKTAFEIDQRWVIELAADRTPDICQSQSVNVFLPGDVDKWD
                     LHMLHWQAWERGMKSLYYLRSKSVQRASHAGAETLDTYSFEAGEKTDYDECLACQ"
     gene            10772..12085
                     /locus_tag="PLO65_00520"
     CDS             10772..12085
                     /locus_tag="PLO65_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRD26762.1"
                     /translation="MTWQEIYAARRLTAAEAANRIESGWRVAMGMALAEPPAILAAIA
                     ARAAADEIDEVKLFYFHSTPHAGSTVLRYDLMDRIRPHCFFLGPIERALLEQGAAEGR
                     RTIDFVPGAFSQAPRLMTDPAPIDAYVATVSPMDRHGYFSLGTSNDYGSAVARSARQV
                     IVEVNRHMPRVRGGMIHVSEVHGIVEHDLPLFENPNRPPAPEDEAIGRIVAGLVDDGA
                     CLQMGIGALPDAICAALNQHRDLGVHTELLSPGLVALMRSGAVTNGRKTTDPGLTIFT
                     FALGDQALYDFIDDNAAVESRPVAYVNDPAVIARNDRVVSVNATLQVDLSGACNSEHL
                     AGRQFSAAGGQLDFVRGAYASRGGRSIIACHSTAKAGTVSRITTRLDGPVTTPRNDTH
                     IVVTEFGHADLKGLSTAGRAKALIGLAHPKFREELTAAAKADGSL"
     regulatory      12118..12289
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            12305..12868
                     /locus_tag="PLO65_00525"
     CDS             12305..12868
                     /locus_tag="PLO65_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRD26763.1"
                     /translation="MEAFLVSTGLVAIAEIGDKTMLLAIVLAARFRKPWPILAGILFA
                     TLANHGLAATLGSVAGHALHGPWVHWVLGLLFIAFGVWALIPDRFEDDEAPSTIRAGS
                     VFLTTAIAFFFVEMGDKTQIATVALAARFHAILLVAAGTTVGMMLANGPAVWLGEAAA
                     TRLPMRYIRWAAAAAFAGIGLWILLAG"
     gene            complement(12873..13373)
                     /locus_tag="PLO65_00530"
     CDS             complement(12873..13373)
                     /locus_tag="PLO65_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD26764.1"
                     /translation="MTDTPADLGTITRLGDQVQVTLTRRFDHAVATVWVMLTDPARLP
                     EWLAPGEIELHEGGRARLDFVDSGTTIDSEVSAFKPGALLEYSWSEPGEPLRPVRWEL
                     QADGPAATRLTLILTTPAAEDAGRAAAGWEAHLEMLAAALEDVPIKFPFERFKAARDA
                     YRALLA"
     gene            13515..13817
                     /locus_tag="PLO65_00535"
     CDS             13515..13817
                     /locus_tag="PLO65_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD26765.1"
                     /translation="MTDAAKPAYVILVRDRMIDPAEFATYGQKAREARAGHAVTPVAY
                     YGRHEVLEGPPVEGAVILQFPSFAEAQAWYRSPAYQEALRHRLAGAEYRVMIVEGV"
     gene            complement(13814..14110)
                     /locus_tag="PLO65_00540"
     CDS             complement(13814..14110)
                     /locus_tag="PLO65_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD26766.1"
                     /translation="MTVYCLAQISITDRAAYDRYQARFMEVFQRFGGRLLAADESPSV
                     EEGRWDRDKVVLMSFPDEAAFRAWADSPAYQEIAKDRRAGSDGVVLLVRGVGGL"
     gene            complement(14115..15113)
                     /locus_tag="PLO65_00545"
     CDS             complement(14115..15113)
                     /locus_tag="PLO65_00545"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRD26767.1"
                     /translation="MSLRTPLCDLLGIEHPIMLAGMGGVSYAELAAAVSNAGGYGVLG
                     MAGTSPEFIREQMRKVRSLTDRPFGVDLLAASPESLTAAVEIIIEEGASSFIAGLGVP
                     MPIMEKLKKAGLKVMVVCGAVKHAVKAEQAGCDAVICQGGEGGGHTGLVGTLPLLAQA
                     VEAVKIPVVGAGGLYDGRGLAACLALGAQGVWMGTRFIASVEAHAGDLYRETILQASD
                     EDTIRTRCYSGKPMRVQKNAYVADWEGRPADIQPFPIQAGISIRNNAMGGIGGQIEGL
                     DPDKSCFAMGQSAGGVHDVLPAAEIVARLMAEAHAAIGRLSALNAASPAVARVTAA"
     gene            complement(15125..15907)
                     /locus_tag="PLO65_00550"
     CDS             complement(15125..15907)
                     /locus_tag="PLO65_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD26768.1"
                     /translation="MAYKPFDLTGKVALVTGGNGGIGLGMAEAMAQAGADIVIWGSNR
                     DKTLAAEGKLTSYGVRVKAQIVDCADEAQVVAGMAEAVTAMGRIDTCIANAGRGRGAA
                     SFAEMSTDVWNYNMALNLDGVFWTLREAARHMVDRAKAGDPGGSLVAISSLSAIEGAA
                     RNEAYATTKGAVISLIKSVAVEHARHGVRANVILPGWIATDMTAGAQGSDVFNSKVIS
                     RVPMRRWGEPEDFGGLAVYLASDASRFHTGDSFVIDGGYAIF"
     gene            complement(15969..16391)
                     /locus_tag="PLO65_00555"
     CDS             complement(15969..16391)
                     /locus_tag="PLO65_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD26769.1"
                     /translation="MSGFPTPTVGVVCLRDDEVLLIKRGNPPRQGQWSLPGGRIEWGE
                     TSEAGALRELVEETGVEAELLGLVDVVDGIMTSRETGRVTRHYIMVDYAARWLSGEPV
                     AGDDAADARFFPFEEALSIVEWDVTRKVIQDAINRFGA"
     gene            complement(16388..17179)
                     /locus_tag="PLO65_00560"
     CDS             complement(16388..17179)
                     /locus_tag="PLO65_00560"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRD26770.1"
                     /translation="MTSQLRGQVAIITGSTSGIGLGLAEALAAAGVNLVLNGLGEAAV
                     IEATRAGLAARHGVEVRYHGADMTRPAEIADLVASTVAHFGRLDILVNNAGIQHVSPV
                     EDFPIDAWDRIIAINLTSVFHATRAAVPVMKAAGRGRIVNIASAHALVASPFKSAYVA
                     SKHGVAGFTKSIALEVAQSGVTINAICPGYVKTPLVEAQIADQAKARGISEEAVMRDV
                     ILAAQPTKQFVTVEQLAGLLLYLCADIGASANGAILSVDGGWTAA"
     gene            complement(17268..18026)
                     /gene="yaaA"
                     /locus_tag="PLO65_00565"
     CDS             complement(17268..18026)
                     /gene="yaaA"
                     /locus_tag="PLO65_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRD26771.1"
                     /translation="MLMVISPAKAMNFMAPERILPLTTPALKDDIAELDVITRKLRPR
                     DLRRLMDISEKLADLNYERFQAFDPAVEDGLQAAIAFNGDVYAGLNARTLDARAFAWA
                     QDHLRILSGLYGLLRPADAIQPYRLEMGVRLKTGRGKTLCQFWGDRIARSLNAAAEGH
                     ADKTLVNLASLEYFAAVDLKALALPVVTCKFFEDRGAVTKQISFMAKKARGMMARYAI
                     DHRIDRAKDLKAFDVDGYVFRGDLSSDTEWIFQR"
     gene            complement(18167..19576)
                     /locus_tag="PLO65_00570"
     CDS             complement(18167..19576)
                     /locus_tag="PLO65_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD26772.1"
                     /translation="MTISVPADVISRLKAVLGEGGWSQDPQRIAPKLVEWRGRWEGTT
                     PLLVLPKTTTEVAAVVGICAQSGTAITPQGGNTGLVSGQIPQGEVLLSTERLNRIRDI
                     DGFDDVIVAESGVTLAAVHEAAAKLSRRFPLGLASEGSATVGGVISTNAGGTQVLRYG
                     TTRNLVLGLEAVLPNGEIWNGLKRLRKDNTGYDLKQLLIGAEGTLGVITAASLKLFPV
                     LTSRATAVVGLGSPADAIKLLARAKEETGGAVEAFELMSRLGVQFAVDHIPGIREPLD
                     AVHPWYVLVETASGEPGAAEGAMERLLARALEDDLIQDAAIAQTQAQARAFWAVRENQ
                     SGAQKPEGAAWKHDVSVPVSRIADFIDQATAAVLKHDPRVRVVAFGHVGDGNVHYDVL
                     RPEGGSDEAHNEGRDAGSLIVHDIVAAMDGSISAEHGLGAAKTADALRYKSSVEVEAL
                     RAIRGVLDPGRIMNPRVLF"
     gene            complement(19608..>19942)
                     /locus_tag="PLO65_00575"
     CDS             complement(19608..>19942)
                     /locus_tag="PLO65_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HRD26773.1"
                     /translation="EALIGPLAEAEADAKRSPGRLALHYAPDAPVRINVEAARPGEAF
                     LAFGPGDSPFNLSPTRNLVEAAANLFAMLRAADRTKPTAIAVAPIPAEGLGEAINDRL
                     KRAAGFVG"
BASE COUNT         3016 a         6968 c         6957 g         3001 t
ORIGIN      
        1 ccatggccgc cagagcctgg tcgagttgcg acgcggcggg gccggcgccg ccggggatct
       61 gaaagcggcc cgcttccggt tcggcgtcgg ggagcagcgt cagactatgg cgcgatcgca
      121 cgggactccg accggcccgg gtggtcacca cccgcgcgcg gaagctgcgg ccccacacgt
      181 ccgtccgcag ggccagggcc acctcgtcga agccctccgc gacgggcgcc tcgaccgtct
      241 cgtgcgacca gaccgccgca agtgcggcga tcgcacgggc gtagtcggtc ctggacaggt
      301 ggaagtccgc cgtcctgtgg accgcgctcc agtcctcgac gcccaggcgc cgggccgtgg
      361 cccgggccgc cgccgagccg ccgatcgcct cgaccagcgc cagcgacacg gggatcgagg
      421 cggtcacccc cgttgtggaa atgacgggtc cgtcctggac gtagcgacgg tcgcgcaccc
      481 aggtcgtggc cggataggcc ttctccagcg ccttcagcgc gtgccagtgg gttgtcgcgt
      541 ggcgcccatg aaacagcccg gcgttggcga ccactcgcgc gccctcgcag atggcgacga
      601 tggtcgcgcc ccggtcggcc tggcgcttca cccagaccgt gagacgcctg tcccgcggct
      661 cgacctgcgc cggaacgatg acgatgtcgg ccccggccgg ctcggcctgg tcgaactgcg
      721 ccagcgtctg gtccgcgcga atcgtcagcg ccatgcgcag ccggacgggg ccctccccgg
      781 ttgacaggct gcggacatcg gcgaccccgg agtccttcag cacgccgtag gggatgatga
      841 agtcggtcac ctcggctccc tggttctcac cgacgacgac gaccagcggc cggacccggc
      901 caggcttggg cgccggcagg gtcagacggt cgccggcgcc gcggcccagg gcgacgaccg
      961 gcgtgtcgcc ggccagtcgt tccgcggcct cggcggccac gaggggaagg gccaggaagc
     1021 tggcgatgat cagcagggga gcgcgcatgg cgaagtctct ccaggggcat gaggcccccc
     1081 ggaaccgcgc ggcgggtcca gggggtgggt cagacgtcag gcggtcggcc cggccgcggc
     1141 gcagggggcg gcgcaggcgc aggcggaacc gcagttgcag gacggcccgc agtcacacga
     1201 cgggcaggtg caggggtcgc aggggcattg gtcggtcatg gtctcgtctc caggtcggcc
     1261 gggggatccg gcgatgagag gaccttcgtg aaaaatggcg ctggcgtaaa tgtcacagat
     1321 atcgcaaatt acgccaatga ccctcgttga cacccgcccc cgcaacatcg ccctcgtcgg
     1381 ctacgccggc gcccagtcgc tcgacctcgt cggtccactg gaagtgtttt ccatggccaa
     1441 caactatggc ggcggcgacc gctaccggcc gatcctcgcc tcgttcgagg gcggggacat
     1501 ccgctgcaat tccggcctca tcctcgccgg cgcgacggcc ctggccgacc tgccggagga
     1561 tctggacacc atcctggtcg cagggggatc ggaggcgggt ctgcgcgggc aggagaacgg
     1621 gccgctgctt gactggctgg ttgctcgccg cgccacgacc cgccgggtcg gcagcgtctg
     1681 ttccggcgcg ctggtgctgg ccgccacggg cctgctcgac ggccgccggg cgaccaccca
     1741 ctggggcgtg tgcgacgtgc ttcaggcgcg ccatcccgag atcgaggtcg acgccgacgc
     1801 catcttcgtc gccgacccgc cgttcttcac ctcggccggg gtcacggcgg gcatcgacct
     1861 gtgcctggcc ctggtcgagg ccgactgcgg gccggacatc gccctggccg tcgcccgcaa
     1921 cctggtgctg ttcatgcgcc gtcccggcgg gcagacccag tacagcgccg gcctcaatgt
     1981 ccaggccgcc gccagcccca gactgcggcg actggtcgtc gacatcacgg ccgacccggt
     2041 cggagacctg agcctgccca gcctcgcggc gcgggccggg atgaccgaaa ggaccttctc
     2101 gcgcgtcttt caccgcgaga caggagccac gccggccgct ttcgtcgaag cggcgcgcct
     2161 caatcgcgcc aaggccttgc tggagtcgtc ggactggccg ctcgctcgcg tcgccgagcg
     2221 ggccggcttc ggcagtctcg acgcgctcca ccgggcgttc cagaagcgtc tgggggcgac
     2281 gcctggagag tatcggcagc gcttcggccg gccgacgacc gcctgatcgg cgccggcggt
     2341 tcaatggacc cggacgtggt gaagactcac aagtcccgcc gccagggcca gggcggcgcc
     2401 aatgccgaag gcgatcgtcg gcgtggcctc gaccgaggtc gtcgcgaaga gcaaggtgac
     2461 cagaacggcc ccggccgtct ggccggtcaa tcgggccgtc ccctgcatgc cgccggccgc
     2521 gccgctccgt tcgggcgggg ctgacagaaa caggttccgg ttgttcgccg cctgaaacag
     2581 tccgaacccc accccgcaga gcgccgtcat cggaatgagg gggcgaaggt cgttctccag
     2641 cggccagagc gccagggcga gcaggcccgc cgccaggccg gcgccgcccg cggcgcagag
     2701 ccagaccatc gaagcccggt ctgcaagacg actgaccacg atcgccgtcc cggcgacggc
     2761 cagcggccag acggtcatgt agaggccggt cgtcagcggc gtctggccga ggccagcctg
     2821 aagttggaag ggcagcgcca gcaggcccgc ggactggccg gcgaagcagc agaccgaggc
     2881 gatcaccgag accctgaacg cgcctgagcg cagcaggtcg agcggaaaga gcggcgtcgc
     2941 cttgggactc tcccgctgga caagcgccgc caggcccagc gcggccacag cgaagagccc
     3001 gacagccaac gccgggcggt ccggcaggag ttcggcgccg acgaccagcg atgcgaacag
     3061 gagggcgttg agcaggacgc tgcccagatc gatcgcctgc ggtcggccga tcaccggcgg
     3121 cagggcgcgg gaggcgaaca ggaccacgac gcccaatgga aggttcaggg cgaacagcca
     3181 gggccagtct gcgcctgtca ggaccagggc cccgatggtt ggcccggccg ccgaggacag
     3241 ggccacggtc atggcgttcc agccgatcgc cgagccgagc cgcccgcgag gaacggaaaa
     3301 gcgtagaagc gcgactccca gcgccatgac ccccgcgccg cccaggcctt gaagaaatcg
     3361 cgcggcgacc aaccagggaa gggacggcgc cagggcgcac agcgccgagg ccagggtgaa
     3421 gatcgtcacg ccccaggtga acatcctccg gtagccatac cgctcgccga gcgccccgca
     3481 cggcaacagg gcgatcacca gggccgtctg gtaggccgtc atgaccagga ccgccgtggg
     3541 cggcgcgacc cgcagcgacc gtgcgatagt cggcagggcg atattcgcga cggaggcgtc
     3601 cagcaccaca agggccatgg atgtcagcac cgccgcagtg gcgaccgcgc ggcggggcca
     3661 cgccaggccg ccgtccgcct tgccgctccg ggcgctcatc gcgccggccg ccccgctcac
     3721 cgcggccgtc ctggagacca gccgccctcc atccgcacat atcgcgtcac ccggtgctcc
     3781 tgcaggtcct cgaccttcag atcgcgttcg gtgaagcgaa agagcagggc cgaggggctc
     3841 aggcagcggg tgtcgtggat gtcgccaggc aggtacgcct tgacctgtcc ggcccgcagc
     3901 acagtcctgt cacgctcgac cagccggatc tcgccgtccg ggttctcgat ccggacatag
     3961 gagcccattt cgatctcgcc ctgctggatc gcgtagacca cccagctccg cccgtgatcg
     4021 tggggcggcc gatagaggcc ggcctgttcg gtgtgggcca gaagcacgaa accgagcgcc
     4081 gggtcgcggt gcagttcggc attggccggc cggtcccggt gcagcgccgc cagccagggt
     4141 tccgcgggcg gcgccttgag ggcggcctcc aacgcccggc tgcacccggt gaccagctca
     4201 ctggtgagcg gaccccaggc ggacctgacg gccgcgacaa acgtgtcgag tgatgtgttt
     4261 tccatcgtct gcgccttcct tctgcgtcaa ggcacgaagc gtaggtcgac cgattagatg
     4321 gcggaacgcg cagcatttgc actatattgc tgcatcaaac gctatgtaac cagggagccc
     4381 gcgatacgcc ccatgacccg cccggacctg aatctgctcg tcacccttga tgcccttctc
     4441 acggagggga gcgttgcgcg ggcggccaga cgcctgcgcc tcagtccttc ggcgatgagc
     4501 cgcgcgctgg cgcggttgcg cgagacgatc ggcgatccgc tgctggtgcg ggcagggcgg
     4561 ggtcttgtgc cgacgccgcg cgccctcgcc ttgcgcgagc aggcccacca gctgctgcag
     4621 gacgtggacg ccgtgctgcg tccggagcag acgatcgatc tggccaggct gacgcgaacc
     4681 ttcaccctgc gcaccagcga cggtttcgtg gagagtttcg gtccgggcct gatcgccagg
     4741 gtcggcgagg acgcgccggg ggtgcggctc cattttctgc agaagccgga caaggacagc
     4801 ggcccgcttc gcgatggccg ggtcgatctg gagaccggcg tggtcgaaag ctcgctcggc
     4861 ccggaggtgc gcacccaggc cctgttccgg gaccgcctga tcggagtcgt gagggcggag
     4921 cacccgctcg gccgcggcgc cgtcacgacc gcccgttacc tggccgagcg ccatgtcgtg
     4981 gtttcccgga gcggcttcga cgacgacgcc gtggatcagc gctttctgcc gcccgccctg
     5041 aagcgccggg tcgccgcgac gattgtcagc ggcttctcgg ccgcgctgtc gctcgcgcgt
     5101 ctctcggacc tggtggcgac ggttccggac cggcatacgg cgaacctgcg cgacggactg
     5161 cacacgttcg ccctgccggc ccccacgctg gatttcgcgg tgtcgatgct ctggcatccc
     5221 aggatggaca gcgatccggc gcaccgctgg ctgcgaggtt gcctgcgcga aatctgcgcc
     5281 ccggccggac cggggctgtc aaccggccga ccctgacggc ccaggcgccg catagtccgc
     5341 ctctatcctg tcgcaagaaa caattgattg tccgactccc gccgcgcggg actaggctga
     5401 cggccgccag cggtcggggg tccgcggcgc cgtcgcggac ccccgacccg atcccgttat
     5461 cggagactca acgaccatgg ccctcgccac cagcaagacc ctcgaaaacc tgaaggccgc
     5521 tttcgccggc gagagccagg cgaaccgccg ctacctctat ttcgcacaga aggccgacgt
     5581 ggagggctac aacgacgtgg ccgcggtgtt ccgctcgacc gccgagggcg agaccggtca
     5641 cgcccacggc cacctcgagt tcatggaggc tgtcggcgac ccggccaccg gcctgccgat
     5701 cggcggcacg ggcgacaacc tcaaggccgc catcgccggc gagacccatg agtacaccga
     5761 catgtatccg ggcatggccc gcaccgcgcg cgaggaaggc ttcgacgaga tcgccgactg
     5821 gttcgagacc ctggccaagg ccgagaagtc ccacgccggc cggttccagc gcgcgctgga
     5881 tacgctggac tagggcgtag cgcataggga gtagggagta gccaggcgat gacgcggccg
     5941 tggcggtgcg cgcaggtcga actactccct actccctagt tcctactccc tttccagagg
     6001 cgcccatgac cgacgaaccc accgcccttc cgcccgcggc cgccagggcc gaaggcagcc
     6061 tcgacgcgcc gatgcggcat ccgatcgcct ggcgggacga cagctattac gacctggcgg
     6121 ccgtcgagaa ggagatggag cgggtcttcg acatctgtca cggctgccgg cggtgcttca
     6181 atctgtgcga cagcttcccc aggctgttcg acatgatcga cgagggaccg accggcgagc
     6241 tcgacggcgt gccgaaggag aaatacgccg aggtggccga ggcctgcacc ctctgcgaca
     6301 tgtgtttcct gaccaaatgt ccgtacgtgc cgccgcatga gttcgacctc gacttcccgc
     6361 acctgatcct gcgctaccgg gccgcgaaac ggcgggcgtc gggtggcgaa ttcgtgcgcg
     6421 agcagctcgg cgaaacggac cgcaacggca agctcgccag gcctgtggcg ggcctggcca
     6481 actgggccac ggcgcgcgag aacacacccc ttcgcggcct gctcgaggcc atagccggca
     6541 tcgaccgcga ggccgagctt ccgaaattcc attcaaggac cgcgaaggac cggctgaaga
     6601 ccatgcccgc cgccgatccc gccggtccgg cctacggcca gcgcaaggcg gtgatctacg
     6661 ccacctgttt cagcaactac aatgacccgg ccacggccga ggccgccgcc gccgtgctgg
     6721 ccaaacaggg ggtcgagacg gccttctgct accctgaatg ctgcggcatg ccccagctcg
     6781 aggccggcga cctgtctgat gtcgcgggcc gcgccgagcg cacggcggca gccttcgcgc
     6841 cctacatcgc ccagggctat gaggtgatcg ccctgaccgc ctcctgcggc ctgatgatga
     6901 agttcgagtg gccgttgatg ctgccggaaa gcgccgcggt ccaggccctg gcgcgggcga
     6961 cgaaggacat ctcgcaatac gtcgtgggcc tgagcaaggc gttcggcctg gcgccgggcc
     7021 tgagccccgt gccgggcggc gtcaccgtgc accacgcctg ccacgcccgg gcccagaaca
     7081 tgggcgccca gtcggcccag atgctgcggc tgatccccga cacccgcatc gagctggtcg
     7141 aacgctgcag cggccacggc ggcaccttcg gggtgatgaa ggccacccgt ccggcggccg
     7201 tcaaggtcgg ccggccggcg gcccggcagg tggcccagaa gaagaccgaa gagatctgtt
     7261 ccgactgccc gctggcctgc acccatcttg gccagatggt cgccgccgag accggcggag
     7321 cccaggtcca caccggccac ccgatcgagg tgctggccaa ggcctacggt ctcgccttcc
     7381 agaattgagt tccgacccat gcccgcctcc gagcaccgca tcaccgccgc cgaccttatc
     7441 cccgacgccg actacgcccg ccagcgggcc gagcggcgcg ccgccctgct gccggtcaag
     7501 cggctgcggc gcgtggccct gggcccctat tgcacggtga tcttcgagaa ctacgacacc
     7561 atgctgttcc aggtgcagga gatgctgctc acggagaagg gcggtccggc ccaggtgccc
     7621 gacgagctgg ccgcctacaa tccgctgatc ccgcaaggta gcgagctggt cgcgacggtg
     7681 atgttcgaga tcgacgagcc gatccgccgc gccgccgccc tggccaggct gggcggggtc
     7741 gaggcgcggt tcttcctgca gttcggcgcc gacctccgcg ccgggggcgt gcctgacgac
     7801 gatgacatcg agcgcacgcg cgaggacggc aagacctcgt cggtgcattt cctgcgcttc
     7861 ccgctcgacg aggcgatgaa ggccgtgttc cgcgacccga ccgtgaccat cgccatcggc
     7921 tgcgaccacg agcactacgc ccatctggcc atgctcagcc cggcgacgcg agcggaactg
     7981 gcgcgggacc tgagttagcg cggggacatg tacccccttg ggtaggtgtc ccctgccgct
     8041 tgccgaaggg gcgagggaca cgcactgaag tgcatgtccc tgatcctact tctttccggc
     8101 gacctcgatg aaccggctgg tcggcgcggc ggcgggcttc ttgtccgcct tgggcttgcg
     8161 gacttccttg ttggacttct tctgaccctt ggccatgacg cgctccggga tggtggcgcc
     8221 ccgggcccgg cacgacgccg ggcgagggtc tgccggcggt gatggcgcag tctacagcat
     8281 ggccctgtcc ccggtcgcgg caaattctcg cccggaaatg aaccgccgcc gggctcgcgg
     8341 attacccctc caggcattcc acggaggcga gcatgcccga caagagcaat atccagggcg
     8401 agggcgacta tgaggccgcc aaacgctttg acgacgccga gcgggccttc gtcgagaccg
     8461 gtccggtcaa gcagaaggcc cgcgaagccg aacaggccct cgacggcccc gaaggcgccg
     8521 agctggagcg ggcacgggtc gaaagtggca agggcgatac gggctaggat gcgaaacgcc
     8581 ggcgcgatcc gatcgcgccg gcgtctgccc ccccgtgccg gatcagccta ctggcaggcc
     8641 aggcactcgt cgtagtcggt cttttcgcca gcttcgaagc tgtaggtgtc gagcgtctcg
     8701 gcgccggcgt ggctggcgcg ctgcaccgac ttggagcgca ggtagtagag gctcttcatg
     8761 ccccgctccc acgcctgcca gtgcagcatg tgcaggtccc acttgtcgac gtcgcccggc
     8821 aggaagacgt tgaccgactg gctctggcag atgtccggcg tgcggtcggc ggccagttcg
     8881 atgacccaac gctggtcgat ctcgaaggcg gtcttgtaga cgtccttgtc gtcctggctc
     8941 aggcaatcga ggtgctgcac cgacccctcg ttggccagga tgctgtccca caccgccggg
     9001 gtgttggcgc ccttggtctc gagcaggcgc tcgaggtagg gattgcgcac cgcgaagctg
     9061 cccgacagcg tcttgtgcgt gtagatgttg gccgggatcg gttcgatgcc ggcgctggtg
     9121 ccgccacaga tgatgctgat gctggcggtc ggggcgatgg ccagcttgtg actgaagcgc
     9181 tccatcacgc cacgctctgc cgcgtcgggg caggggccct tctcctcggc cagcttgacg
     9241 ctcgccttgt cggcctcgcg gcgcagatgc ttgaacagcc gcatgttcca cgacttggcc
     9301 agggcgctct cgaacggcac gttctgagcc tgcaggaagc tgtggaaacc catcaggccc
     9361 aggccgaccg accgctcgcg ctgggcggcg tagatggcgt gcttcgcatc ctgcggcgcg
     9421 cgcttgatga agtcttccag cacattgtcg aggaagcgca tcacgtcctc gatgaagcgc
     9481 ggctcgtcgc gccattcgag gaaggtctcg gcgttgacgc tcgagaggca gcagaccgcc
     9541 gtgcgctcgg cgcccagatg atccttgccg gtgtgcagca tgatctcgct gcacaggttg
     9601 gactggcgca cctgcaggcc cagctcgcgc tggtgcctgg gcatcgccct gttcacatgg
     9661 tcggcgaaga tcaggtaggg ctcgccggtc tggagccgca gctcaagcac cttctgccac
     9721 agggcgcggg cgtcgacctc gcgcagcact tcgccggtct tgggcgagcg cagtccgaac
     9781 ttcgtgccgt cacggaccgc gtgcatgaac tcgtcggtga tcgagatgcc gtggtgcagg
     9841 ttgagggact tgcggttgaa gtcgcccgac ggcttgcgga tctcgaggaa ctcgtcgatc
     9901 tcgggatggt ggatgtcgag gtagaccgcc gccgagccgc ggcgcaggct gccctggctg
     9961 atggccagtg tcaggctgtc catcacccgg atgaagggga tgatgccgct ggtctggccg
    10021 gcgcccttga ccttctcgcc gatcgagcgg acgccgcccc agtaggtgcc gatgccgccg
    10081 ccattggcgg ccagccagac gttttcgttc caggtgccga cgatgccgtc gaggctgtcg
    10141 ccgacagcgt tgaggaagca gctgatcggc aggccgcgat cggcgccgcc gttcgacagc
    10201 accggcgtgg ccggcatgaa ccacagccgg ctcatgtagt cgtagacccg ctgggcgtgt
    10261 tcggcgtcat cggcgaaggc cgtggccacc cgggcgaaca tgtcctggta ggactcgccg
    10321 gcgagcaggt agcggtcctc cagcgtggtc ttgccgaaat cagtcagcag ggcgtcccgg
    10381 ctacggtcga cctcgacctt gcgcaccagc gccagctggg gtttttcgcc gcctgcccag
    10441 gccgtgtcgc cggccgcctc gccgagggga atctgccttg ccgcttcgcc catcataacc
    10501 ctaaaacccg taatccacag cggcttccag aacgaacgca ccgcccggaa gacgctacat
    10561 cttgtgggcg acgcgccccc tgaacccact atatggggcc acgagggcta atgactcgtc
    10621 aacggggaga aacgcctcag gggcgacttt ttcgttaaca acatgttgag aagccgtccg
    10681 tcgacggcgc tcccgcaagg gttcggcaac catttcagac acctgccgcg acccctcgtc
    10741 gcggaggccc cgccgtggca aggtcgccgt gatgacatgg caggaaatat acgccgccag
    10801 gcgcttgacg gccgccgaag cggccaaccg catcgagtcc ggctggcgcg tggcgatggg
    10861 catggccctc gccgagccac cggcgattct cgcggcgatc gccgcccggg ccgcggctga
    10921 cgagatcgac gaggtcaagc tcttctattt ccactccacc ccgcacgccg gctcgacggt
    10981 gctgcgctac gacctgatgg atcgcatccg gccgcactgc ttcttcctcg ggccgattga
    11041 acgggcgctg ctcgaacagg gcgcggccga gggcaggcga accatcgact tcgtgcccgg
    11101 cgccttcagc caggctccgc ggctgatgac cgacccggcc ccgatcgacg cctatgtcgc
    11161 caccgtctcg ccgatggacc ggcacggcta cttcagcctc gggacgagca acgactacgg
    11221 ctcggcggtg gcccggtcgg cgcgccaggt gatcgtcgag gtcaaccggc acatgccccg
    11281 cgtgcgtggc ggcatgatcc acgtcagcga ggtccacggc atcgtcgagc atgacctgcc
    11341 gctgttcgag aaccccaacc ggccgcccgc gccggaggac gaggcgatcg gcaggatcgt
    11401 cgccggcctt gtcgatgatg gggcctgcct gcagatgggc atcggagcct tgcccgacgc
    11461 gatctgcgcg gccctgaacc agcatcggga cctcggcgtc cacaccgaac tgttgtcgcc
    11521 gggcctcgtg gcgctgatgc gctcgggggc ggtgaccaac gggcgcaaga ccaccgaccc
    11581 gggcctgacg atcttcacct tcgcgctcgg cgaccaggcc ctgtacgatt tcatcgacga
    11641 caacgccgcg gtcgagagcc gcccagtggc ctatgtcaat gatccggcgg taatcgcccg
    11701 caacgaccgc gtcgtttcgg tcaacgccac gcttcaggtc gacctgtcgg gcgcctgcaa
    11761 ttccgaacac ctggccggcc gtcagttcag cgcggccggc ggccagctgg atttcgtccg
    11821 cggcgcctac gcctccaggg gcggccggtc gatcatcgcc tgccattcga cggccaaggc
    11881 cgggacggtc tcgcgcatca ccacccggct cgacggcccg gtgaccacgc cgcgcaacga
    11941 cacccacatc gtcgtcaccg aattcggcca cgccgacctc aagggcctgt cgacggccgg
    12001 gcgggccaag gcgctgatcg gcctcgccca tccgaagttc cgcgaggagc tgacggccgc
    12061 cgcaaaggcc gacggcagcc tgtagccttg ccaatcgggc cggagaaccg tatctgccgc
    12121 gctgtcactg gggagtagcc ggctgcgcgt tcagcagcgg gtgcgtcaac agacttgggg
    12181 cctggctcca tggcgcatcc agtccgagag gacctggcga gacctttggc tttcgcgtgc
    12241 ggtcgagcgg gaccggagcg cggaacgcca ttggtctgtc ttaccggtcc cgcaggacac
    12301 gttcatggaa gccttcctcg tctccaccgg cctcgtggcc atcgccgaga tcggcgacaa
    12361 gaccatgctg ctggccatcg tgctggcggc gcggttccgc aagccatggc cgatcctggc
    12421 cggcatcctc ttcgccaccc tggccaacca cggcctggcc gcgacgctgg ggtccgtcgc
    12481 cgggcatgcc ctgcacgggc cgtgggtgca ctgggtgttg ggcctgctgt tcatcgcctt
    12541 cggcgtctgg gcgctgatcc ccgataggtt cgaggacgac gaggcgccgt cgacgatccg
    12601 ggccggatcg gtcttcctga ccaccgccat cgccttcttc ttcgtcgaga tgggcgacaa
    12661 gacgcagatc gcgaccgtgg ccctcgccgc gcgcttccac gcgatcctgc tggtcgccgc
    12721 cggcacgaca gtcggcatga tgctggccaa cgggccggcc gtctggctgg gcgaagcggc
    12781 ggcgacgcgg ctgccgatga gatatatccg ctgggcggcc gccgcggcct tcgccgggat
    12841 cgggctgtgg atcctcctcg ccggctagcg ggtcaggcca gcagggcgcg ataggcgtcg
    12901 cgggccgcct tgaaccgctc gaaggggaat ttgatcggca cgtcctcgag ggccgccgcg
    12961 agcatctcaa ggtgcgcctc ccagcccgcc gcggcgcggc cggcgtcctc ggcggccggc
    13021 gtcgtcagga tcagggtcag gcgggtcgca gccggcccgt cggcctggag ctcccagcgc
    13081 acgggccgca ggggctcgcc cggttcgctc cagctgtatt ccagcagggc gcccggcttg
    13141 aaggcgctga cctcgctgtc gatggtcgtg cccgagtcga cgaagtccag gcgcgccctg
    13201 ccgccctcgt gcagctcgat ctcgcccgga gccagccatt ccggaagccg ggccgggtcg
    13261 gtcagcatga cccacaccgt ggccaccgcg tggtcgaagc ggcgggtcag cgtcacctgc
    13321 acctgatcgc cgagccgggt gatcgtgccg aggtcggcgg gcgtatcggt catggcggcg
    13381 ggctcctgcg gggtgtggcg caggcctagc gccggtccgc cggccggacc gtgacctgaa
    13441 tcaacctccg ccacatcgcc agcgtcggcg gggacggcta gaccgtggaa cgcccgcaag
    13501 tccggagatt cgccatgacc gacgccgcca agcccgccta tgtgatcctc gtccgcgacc
    13561 ggatgatcga ccccgccgag ttcgccacct acggccagaa ggcgcgcgag gcgcgcgccg
    13621 gccacgcggt cacgccggtg gcctactacg gccgccatga ggtgctggag gggccgccgg
    13681 tcgagggcgc ggtcatcctt cagtttccca gcttcgccga agcccaggcc tggtaccgca
    13741 gcccggccta ccaggaggcc ctgcggcacc ggctggccgg cgccgagtac cgggtgatga
    13801 tcgtcgaggg cgtctagagg ccgcccacgc ccctgaccag caagaccacg ccgtccgagc
    13861 cggcgcgccg gtccttcgcg atctcctgat acgcgggcga atcggcccag gcccggaagg
    13921 ccgcctcgtc cgggaagctc atcaggacga ccttgtcgcg gtcccagcgg ccttcctcga
    13981 ccgacggcga ctcgtcggcg gccagcagcc ggccgccaaa ccgctggaag acctccatga
    14041 agcgcgcctg gtagcggtcg taggcggcgc ggtcagtgat ggagatctgg gccagacagt
    14101 agaccgtcat gaggtcaggc cgcggtgacc cgcgccacgg ccgggctggc ggcgttcagg
    14161 gctgacaggc ggccgatggc ggcgtgcgcc tcggccatca ggcgcgccac gatctcggcc
    14221 gctggcagga catcgtgcac gccgcccgcg ctttgaccca tggcgaagca tgacttgtcg
    14281 gggtcgaggc cctcgatctg gcccccaatg ccgcccatgg cgttgttgcg gatgctgatg
    14341 ccggcctgga tcgggaaggg ctggatgtcg gccggccggc cttcccagtc ggcgacatag
    14401 gcgttcttct gcacccgcat cggcttgccg ctgtagcagc gggtgcggat ggtgtcctca
    14461 tcggaggcct gcaggatggt ttcccggtac aggtcgcccg cgtgcgcctc gacactggcg
    14521 atgaagcggg tgcccatcca gacgccctgg gcgccgagcg ccaggcaggc ggccaggccg
    14581 cggccgtcat agaggccgcc ggcgccgacc acggggatct tcaccgcttc cacggcctgg
    14641 gccagcaggg gcagggtgcc gaccaggccg gtgtgtccgc cgccttcgcc gccctggcag
    14701 atgaccgcgt cgcagccggc ctgctcggcc ttcaccgcgt gcttcaccgc gccgcagacg
    14761 accatgacct tcagtccggc cttcttcagc ttctccatga tcggcatggg cacgcccagg
    14821 ccggcgatga aggaggacgc gccttcctcg atgatgatct ccaccgcggc ggtcaggctc
    14881 tccggcgagg ccgccagcag gtccacgccg aacggccggt cggtcaggga ccggaccttg
    14941 cgcatctgct cgcggatgaa ctcggggctg gtgccggcca tgcccagcac gccatagccg
    15001 ccggcgttgc tgacggccgc ggccagttcg gcgtaggaaa cgccgcccat gcccgccagc
    15061 atgatcggat gctcgatgcc gagcaggtcg cagaggggag tgcgcaggga catcgctgtg
    15121 atcctcagaa gatcgcgtag ccgccgtcga tcacgaagga atcgccggtg tggaagcggg
    15181 aggcgtcgga cgccaggtag acggcgaggc cgccgaaatc ctcgggctcg ccccagcgtc
    15241 gcatcggcac gcggctgatg accttgctgt tgaacacgtc gctgccctgg gcgccggcgg
    15301 tcatgtcggt ggcgatccag ccgggcagga tcacattggc gcggacgcca tggcgggcgt
    15361 gttcgacggc caccgacttg atcagggaaa tgaccgcgcc cttggtggtg gcgtaggcct
    15421 cgttacgggc ggcgccctcg atggccgaca gggatgagat ggccaccagc gaaccgccgg
    15481 gatcgcctgc cttggcccgg tcgaccatgt gcctggccgc ctcgcgcagg gtccagaaca
    15541 ccccgtccag gttcagggcc atgttgtagt tccacacgtc ggtggacatc tcggcgaagg
    15601 aggcggcgcc ccggccccgg ccggcgttgg cgatgcaggt gtcgatccgg cccatggcgg
    15661 taaccgcctc ggccatgccc gcgaccacct gggcctcgtc ggcgcagtcg acgatctggg
    15721 ccttgacccg gacgccgtag ctcgtgagct tgccttcggc ggccagggtc ttgtcgcggt
    15781 ttgatcccca gatgacgatg tcggcgccgg cctgggccat cgcctcggcc atgccaaggc
    15841 cgatgccgcc gttgccgccg gtgacgagcg cgaccttgcc ggtcaggtca aagggcttgt
    15901 aggccatggt gcgtcactcc ccgcgaacat ctgtttgatg tcacaaaagg cgggtgacgc
    15961 ggcggcggtc aagcgccgaa acggttgatc gcgtcctgga tgaccttgcg ggtgacgtcc
    16021 cattcgacga tcgacagggc ttcctcgaat ggaaagaagc gcgcgtcggc ggcgtcatcg
    16081 ccggctaccg gctcccccga cagccagcgc gctgcgtagt cgaccatgat gtagtgccgc
    16141 gtcacccggc ctgtctcgcg cgaggtcatg atcccgtcga ccacgtcgac gaggcccagc
    16201 agctcggcct cgacgccggt ttcctcgacc agctcacgca gggcgccggc ttcgctggtc
    16261 tcgccccatt ctatgcggcc gcccggcagg ctccactggc cctgccgggg cggattgccg
    16321 cgcttgatca gaaggacctc gtcgtcgcgc aggcagacga cgcccacggt gggcgtggga
    16381 aagccgctca cgccgcggtc cagccgccgt ccaccgacag gatcgcgccg ttggccgagg
    16441 cgccgatgtc ggcgcagaga tagagcagca acccggccaa ctgctcgacg gtgacgaact
    16501 gcttggtcgg ctgggcggcg aggatcacat cacgcatgac ggcctcctcg ctgatgccgc
    16561 gcgccttcgc ctggtcggcg atctgggcct cgaccagcgg agtcttcaca tagccggggc
    16621 agatggcgtt gatcgtcacc ccgctctggg cgacctccag ggcgatcgat ttggtgaagc
    16681 cggccacgcc atgcttggac gcgacgtagg cgctcttgaa tggcgaggcc accagggcgt
    16741 gggccgaggc gatgttgacg atccggccgc ggccggcggc cttcatcacc gggacggcgg
    16801 cgcgggtggc gtggaaaacg ctggtcaggt tgatggcgat gatccggtcc caggcgtcga
    16861 tcgggaagtc ctcgaccggg gagacgtgct ggatgccggc gttgttgacc aggatgtcca
    16921 gccgaccgaa gtgggcgacg gtcgaggcca cgaggtcggc gatctccgcc gggcgcgtca
    16981 tgtcggcgcc gtggtagcgc acctcgacgc cgtgacgcgc ggccagaccg gcgcgggtcg
    17041 cctcgatgac ggcggcctct cccaggccgt tgagcacgag gttcaccccg gcggcggcca
    17101 gcgcctcggc gaggcccagg ccgatgccgc tggtcgatcc ggtgatgatg gccacctggc
    17161 cgcgcagttg ggaggtcatg ggcgatagcg ctccgccttg tttttgttgc ggtgcagcat
    17221 agagggtcgg ggcggccggg gcgagcggaa atcggcgcgc gcggcggtca gcgctggaag
    17281 atccattcgg tgtccgagga caggtcgccg cggaagacgt agccgtcgac gtcgaaggcc
    17341 ttgaggtcct tggcgcggtc aattcgatgg tcgatggcgt agcgggccat catgccgcgg
    17401 gccttcttgg ccatgaagct gatctgcttg gtcaccgcgc cgcgatcctc gaagaacttg
    17461 caggtgacca ccggcagcgc cagcgccttg aggtcgacgg cggcgaaata ttcgaggctg
    17521 gcgaggttga cgagggtctt gtccgcgtgg ccctcggccg ccgcgttgag gctgcgggcg
    17581 atgcggtcgc cccagaactg gcacagggtc ttgccgcggc cggtcttcag ccgcacgccc
    17641 atttccagcc ggtagggctg gatcgcgtcg gcgggccgca acaggccgta gagacccgac
    17701 aggatgcgca ggtggtcctg ggcccaggcg aaggcccttg cgtcgagcgt gcgggcgttc
    17761 agcccggcgt agacgtcgcc gttgaaggcg atggccgcct ggaggccgtc ctccacggcg
    17821 gggtcgaagg cctggaagcg ctcgtagttc aggtcggcca gcttctcgga gatgtccatc
    17881 agccgccgca ggtcgcgcgg ccgcagcttg cgggtgatga catccagctc ggcgatgtcg
    17941 tccttcagcg ccggcgtggt caggggcagg atgcgctcgg gcgccatgaa attcatggcc
    18001 ttggcgggcg agatgaccat cagcatggcg tggggataga gcgggccgaa gcggatggcc
    18061 agtcgcatca tgcccggtcc ggcatccggc ccgcgaccgt cgccgttccg gacccacgcc
    18121 gattgacgcc atcccgggca gacccggagg gtcgcgtcga aggtcgctag aacagcaccc
    18181 gggggttcat gatccgacca gggtccagca cgccccggat cgcgcgcaac gcctccacct
    18241 cgacgctcga cttgtagcgc agggcgtcgg cggtcttggc ggcgcccagg ccgtgttcgg
    18301 cgctgataga gccgtccatg gcagcgacga tgtcgtggac gatcagcgag ccggcgtcgc
    18361 ggccctcgtt gtgcgcctcg tccgagccgc cttccgggcg cagcacgtcg tagtggacat
    18421 tgccgtcgcc gacgtggccg aaggccacga cccgcacgcg cgggtcatgc ttcaacaccg
    18481 ccgccgtcgc ctggtcgatg aagtcggcga tgcgcgagac cggcacggac acatcgtgct
    18541 tccaggccgc gccctcgggc ttctgggcgc ccgactggtt ctcgcgcacg gcccagaaag
    18601 ccctggcctg ggcctgcgtc tgggcgatgg cggcatcttg gatcagatcg tcctccagcg
    18661 cccgggcgag cagtcgctcc atcgcgccct ccgccgcgcc gggctcgccg ctggccgtct
    18721 cgaccagcac ataccaggga tggacggcgt ccagcggctc gcggatgcca gggatgtggt
    18781 caacggcgaa ctgcaccccc agccggctca tcagctcgaa ggcctcgacc gcgccgccgg
    18841 tctcttcctt cgcgcgggcc agcagcttga tggcgtcggc gggcgagccc agaccgacca
    18901 cggcggtggc ccgcgaggtc agcaccggaa acagcttcag gctggcggcg gtgatcacgc
    18961 ccagcgtccc ctcggcgccg atcagcagct gcttcaggtc atatccggtg ttgtccttgc
    19021 gcagccgctt gaggccgttc cagatctcgc cgttgggcag cacggcctcc aggccgagca
    19081 ccaggttgcg cgtcgtgccg tagcgcagca cctgggtgcc gcctgcgttg gtcgagatca
    19141 ccccgccgac tgtcgccgag ccctccgacg cgaggcccag ggggaagcgc cgcgacagct
    19201 tcgccgccgc ctcgtgcacg gccgccaggg tcacgccgga ctcggcgacg atcacgtcgt
    19261 cgaagccgtc gatgtcgcgg atgcggttga gccgctcggt cgacagcagg acctcgccct
    19321 gcgggatctg gcccgacacc aggccggtgt tgccgccctg gggcgtgatc gccgttcctg
    19381 actgggcgca gatcccgaca accgcggcga cctcggtggt ggtcttcggc aggaccagca
    19441 atggcgtggt cccctcccac cggccgcgcc attcgaccag cttcggcgcg atgcgctgcg
    19501 ggtcctggct ccagccgcct tcgccgagca cggccttgag gcgggagatg acgtctgcgg
    19561 gcacggagat ggtcatggga gaagcataac ccgccccgtg gcggccgtca gccgacgaag
    19621 cccgccgcgc gcttcagccg gtcgttgatc gcctcgccga gcccctcggc cgggatcggc
    19681 gcgacggcga tggcggtggg cttcgtccgg tcggccgcgc gcagcatcgc gaagaggttg
    19741 gccgcggcct cgaccagatt ccgggtcggg ctcaggttga acgggctgtc gccggggccg
    19801 aaggcgagga aggcctcgcc gggacgggcc gcctcgacgt tgatccgcac cggcgcgtcc
    19861 ggtgcgtaat gcagcgccaa ccggccgggc gagcgcttcg cgtccgcctc ggcctcggcc
    19921 agtgggccga tcagcgcctc ga
//
LOCUS       DAORNX010000006        18667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2277_length_18667_cov_8.410488, whole genome shotgun sequence.
ACCESSION   DAORNX010000006 DAORNX010000000
VERSION     DAORNX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18667
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2277_length_18667_cov_8.410488"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLO65_00580"
     CDS             <1..1165
                     /locus_tag="PLO65_00580"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HRD26774.1"
                     /translation="ARLARQACANLGGVGVAEVEEVWHALAPPYDDLFAWLEGRSDRP
                     SADHPGRFRPVMLAQPVDEAVDFARLDPADYAAEWKWDGIRIQAVRERGEARLYTRTG
                     DDISHAFPDVLAAMSFEGALDGELLIMREGRVAPFGDLQQRLNRKTVDAKLLAAHPAG
                     IRAYDLLLDGEEELRALSFRERRIRLESFILAANSPRIDLSPLQPFETWESLAALRAA
                     PPEGDPAVAEGLMLKRWDSPYEAGRPKGPWFKWKRDPHLIDAVLMYAQRGHGKRSSFY
                     SDFTFGVWREDANGDRTLTPVGKAYFGFTDEELKRLDRFVRDNTIDRFGPVRSVRATR
                     DFGLVLEVAFEGLQRSTRHKSGVAMRFPRISRIRWDKPAREADELATLERMLD"
     gene            complement(1339..2499)
                     /locus_tag="PLO65_00585"
     CDS             complement(1339..2499)
                     /locus_tag="PLO65_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRD26775.1"
                     /translation="MSIAFIDLAAQQRRIRSRLDAAIARVLDHGGYVMGPEVRQFEAE
                     LAAFGEAPLALSCANGTDAIALPLMAWGIGAGDAVFCPSFTFTATPEVVPWVDATPVF
                     VDVLPDTFNLDPAKLEAAIVAVKAEGKLKPAVVIAVDLFGQPADYPALRAVCDRHGLK
                     LISDSAQGFGCTLNGHHPIHWADVATTSFFPAKPLGCYGDGGAVLLKDQALWDQMDSY
                     RVHGKAVAQDLNGRVFDHDPKYLNARIGMNSRLDSIQAAILIEKLAIFAEEIAMRQGV
                     ADRYAEGLKGSVLSTPKVIAGGQSVWAQYVIEHENRDGLAAHLKTQGVPTAVYYPVPM
                     HVQAPYADYPRGAGGLPVTEALADKVLALPMHPYMDEETQQRIIDAVKGFNG"
     gene            2603..3526
                     /locus_tag="PLO65_00590"
     CDS             2603..3526
                     /locus_tag="PLO65_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRD26776.1"
                     /translation="MQFDAEVDQRLRQTLLGDESLLHKFTDAAGDLAVNLVIGTIILV
                     LTLWASSWFAGLTRRAMNRLPGRRTPDLTLQTFVASMVRYVILVVGFVAVLQQLGVKT
                     TSIVAVLGAASLAVGLALQGALSNVAAGVMILLFRPYRVGDLIETGGKKGIVRALDLV
                     ITELATADNLKIVIPNNKVFGDVIVNHSHHSQRRVDVTFRIPAAADMAGIMAGVLARA
                     RANPLVLDDPEPSVSVTLLAEAAVEIEVLAWTRSADHAAVRADLLLTAKLIEAGQDAS
                     LPPLPKAPLSPPAPAKGPPAGRRSPRSRPTA"
     gene            complement(3456..4625)
                     /locus_tag="PLO65_00595"
     CDS             complement(3456..4625)
                     /locus_tag="PLO65_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRD26777.1"
                     /translation="MTELLKNRLAFATRTIHGGQAHDPTTGAVMVPIYATSTFAQESP
                     GVHKGFEYARSHNPTRFAFERCVANLESGVAAFAFASGLAAASTTLELLDAGAHVIAS
                     DDLYGGSYRLMQRVRARSAGLTFSFVDMTDPAAITAAIRPETKMIWVETPTNPTLRLV
                     DLAAVAAIGKRHGLITVADNTFASPWIQRPLEHGFDLVLHSTTKYLNGHSDMVGGVVA
                     VGENQAIEEQMRFLQNAVGAVSGPFDSFLALRGLKTLALRMERHCQSALRIARWLEAR
                     DDVTRVIYPGLESHPQHALARRQMNGFGGIISVEIAGDLARARRVLERTQLFTLAESL
                     GGVESLIEHPAIMTHASIPPEQRAAIGISDTLIRLSVGIEDCDDLLADLSQALAG"
     gene            complement(4757..6136)
                     /locus_tag="PLO65_00600"
     CDS             complement(4757..6136)
                     /locus_tag="PLO65_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HRD26778.1"
                     /translation="MTLPATTRSALDLIGDTPMVEVTHIDTGVCRLFLKLENQNPGGS
                     IKDRIARAMIEAAEADGRLKPGGTIVEATAGNTGLGLAQVATVKGYRLILVVPDKMAR
                     EKILHLRAMGVDVRITRSDVGKGHPEYYQDMAQTIAQATGGFFVNQFENPANPLAHEM
                     TTGPEILEQMGGDVDAVVVGVGSGGTLTGLGRFFKKHSPKTKMILADPVGSILYDYVN
                     TGTYGEAGSWIVEGIGEDFIPDNAQMDLVAKAYAIPDRESVESARLLLEKEGILAGSS
                     SGTLLAGALRYCREQTTPQRVVSLVCDTGGKYLTKMFNDMWVAAHGFEDRPRSGDLRD
                     LIARDFSEGGVIATGPDDSLLTAYNRMRAADISQLPVVDHGRLVGILDESDILAAVGG
                     REGRADRFGARVASAMTRDVNTLQVHQSLDELKPLFDRDEVAVVMDGEKLVGLITRVD
                     LINHLRRTA"
     gene            complement(6211..6750)
                     /locus_tag="PLO65_00605"
     CDS             complement(6211..6750)
                     /locus_tag="PLO65_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD26779.1"
                     /translation="MSPPRPVMVIFDRDGVLNVDHGYAFDPAALEWVPGARGAVRRVN
                     EAGAVAVIATNQSGIGRGYYTEADMRAFHAEMMRQLAAAGARIDAIYFCPFHEAATED
                     RYRVADHPDRKPNPGMLLRALADFAVVPADAVMIGDKPSDLEAARRAGVRGVFYEGGD
                     LDALVADELALLTGTPFPK"
     gene            6996..9440
                     /locus_tag="PLO65_00610"
     CDS             6996..9440
                     /locus_tag="PLO65_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26780.1"
                     /translation="MALLGLFPFDRAAANNECGFPQAREVTCTAEMNPHANGIHYATQ
                     NNLTINLASGVIVRTSNPTARGVQIIAGSASGARLSIFGGPGVSIETSGDSAIGVLAF
                     SPLGSATVHVDSVRTSGDLSTGVHVVSILTDVRVHNVTTTGNDAPGVFARGHESVTVV
                     VADAIDTSGVNSRGVMATGRMTVEVVNAGDISTAGAFSEGIYAVSDGPDPGGSVVVNS
                     RNTSARDAVAIYAESAHGPTALTVTGTTISARHDAIIAIGETGASVAVESGATVLGGL
                     NAIVIRSGTGAATSPAEAALPARSTVTNAGVITAGDGFAILASGAPVQIRNSGTINGR
                     ISLTDNDDLVVNTGTFNAEGRSEFGAGADGFRNDGVLRAQTASLVTFAGVESFSNSGL
                     IDLRNGQAGNSLVLTGVFVGSGQSQLALDVVLDATGSASDLLVVGSATGETEVLLAVS
                     GGPARLNPGQLLIQSGAASSPTAFRLADSSADQGLIHFSIIYDPTTFDYSLVGVPGAS
                     VYRMAKYSEGAANTWLAASDAWRAGLQSRRDGGGDGPRTWLQVQGSEIERDSLSRTTV
                     FGQTSSFDLSYGQTSRGAQFGAEGSPAAIPGLLLGVTGGLIDSRVSFTNSREEARFET
                     LNFGGYASFSAGSTFVGMLAKYDWSRIDSDGWGQGPARSIDGEAWGITVEVGHRAGSS
                     ALFVEPVITVSWLGTRLEDFTVQGSSIGFEDAEHLRGSLGFRMGGDLGPTVALYGGAV
                     MVREFEGKDGLTFHDSTATLEIANESMPDYSQGHIGLRFSPAAAVSGFVEGFAIFGGD
                     DGVGGGGGRVGVSARF"
     gene            9674..10591
                     /gene="cysD"
                     /locus_tag="PLO65_00615"
     CDS             9674..10591
                     /gene="cysD"
                     /locus_tag="PLO65_00615"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRD26781.1"
                     /translation="MTALVSPARLTHLQRLEAESIHIMREVVSETERPVMLYSIGKDS
                     AVMLHLAAKAFYPSKPPFPLLHVDTTWKFQAMYEMRERMARELGFDLLVHKNPDAEAR
                     GINPFDHGPAHTDLWKTEGLKQALEKHGFDAAFGGARRDEEKSRAKERIFSFRSAEQR
                     WDPKNQRPELWRLYNARKNRGESIRVFPISNWTELDIWQYIHLENIPIVPLYFSGVRP
                     VVERDGTLIMVDDDRFRLRDGETPMMKSVRFRTLGCYPLTGAVESTASTLPEIIQEML
                     LTTTSERQGRVIDHDQAASMEKKKQEGYF"
     gene            10591..12486
                     /gene="cysN"
                     /locus_tag="PLO65_00620"
     CDS             10591..12486
                     /gene="cysN"
                     /locus_tag="PLO65_00620"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRD26782.1"
                     /translation="MSSAYKPADLIETDIDAYLLQHQHKSLLRFITCGSVDDGKSTLI
                     GRLLYDSKMIFEDQLAALEADSRKVGTQGGAIDFALLVDGLAAEREQGITIDVAYRFF
                     ATDSRKFIVADTPGHEQYTRNMVTGASTADAAVVLIDARKGVLTQTRRHSYLVNLLGI
                     RHIVLAVNKMDLVGWDQSIFDAIVEEYSAFADQIGLADFTAIPMSALDGQNITEPSAA
                     APWFDGPPLMRWLETVAVEDDLPSRAFRMPVQWVNRPNLDFRGFSGQIAAGTVRPGDR
                     VKVLPSGRESRVARIVVLPGDLDQAVAGQSVTITLADEVDVSRGDVLVGADDPCPVAN
                     QFESTLVWMDDEPMLPGRPYLMKIGAQTVTASITEPKYRVQVNTLEHIAAKRLELNEI
                     GVCNLSLDRAIPFEAYADNRDLGGFILIDRLSNRTVGAGMLHFALRRADNIHWQATDI
                     DKAARATQKGQKGRVVWFTGLSGAGKSTIANLVEKRLHALGRHTYLLDGDNVRHGLNK
                     DLGFTEEDRVENIRRVAEVSKLMVDAGLIVLTAFISPFRAERRMAREMLEDGEFVEVY
                     VETPLAVAEQRDVKGLYRKARAGELKNFTGIDSPYEEPENAELTVDTTTLSPVEAAEA
                     IVAWLEK"
     gene            complement(12570..13583)
                     /locus_tag="PLO65_00625"
     CDS             complement(12570..13583)
                     /locus_tag="PLO65_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRD26783.1"
                     /translation="MKVMVTGSAGFIGSATTLRLLARGDTVIGVDSLNDYYDPALKRA
                     RLARHIDHPDYTQLTIDLADRAAVEQAFAEHRPQRVINLAAQAGVRYAATNPHIYVQS
                     NVTGFLHILEGCRHNGVEHLVFASTSSVYGANTKMPFSEHDSTEHPLTLYAATKKANE
                     QMAHSYANLFDLPCTGLRFFTVYGPWGRPDMALFLFTKAILAGEPIKVFNHGKHSRSF
                     TYIDDIVEGVIRAMDNVPAKNPLWDGQNPDPATSGSGPYRIFNIGAEENVELLRYIEV
                     LEDCLGRKAIMEMLPLQPGDVPDTVADVTELQRIVGYKPRVTVEEGVRNFVDWYRGYN
                     GVL"
     gene            complement(13602..14885)
                     /locus_tag="PLO65_00630"
     CDS             complement(13602..14885)
                     /locus_tag="PLO65_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRD26784.1"
                     /translation="MDALSLTNPRICVLGLGYVGLPLAVAFGVHHDTVGYDLDAGRIA
                     ELRDGHDRTLEVEPEAMAAAPRLRFSASPADLEDRNVFIVTVPTPIDEARRPDLTAIS
                     GASRAIGACLKPGSVVIYESTVYPGCTEEVCVPILEAESGLRFNVDFFCGYSPERANP
                     GDRQHRLSTITKVTAGSTPEAAAFVDGLYRAIVPAGTHLASSIKVAEAAKVIENTQRD
                     LNIALINEFAMIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQ
                     EIGHHPEVILAGRRINDAMSAYVAGQVLKLLAHRGINPVGARALVLGLAFKENCPDLR
                     NTKVVDLVAELRDYSLAVDVHDPWVDPAEAAHEYGLTLTADPARAAYDVVILAVAHRQ
                     FVDLGAVGVRAFGKPASVLYDIKSALPRDAVDGRL"
     gene            15025..16083
                     /locus_tag="PLO65_00635"
     CDS             15025..16083
                     /locus_tag="PLO65_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRD26785.1"
                     /translation="MVARPTAILFLTALAGCSTVEPVVALPSADRDALRAEETFQQEA
                     SLDRLRQQDGRVAAVSFRLNVANLDLCADRALLTGMVLHHALQYGPDIRPVAIRHFGL
                     TGRPAVLTVAPDSPAARAGIQVGDSLVAIDGSAFANAGDRLARDPPTYDETSKARALL
                     DGALADGAGVLTVERDGRPLTLALTPVAGCAYEAQLTPSEDVSAWADGRRVVITTAFV
                     RYAATDDQLAVILGHELGHNVMKHRARIAGGGPAGALLGNAGTQRGGLLTVEREADYV
                     GLYLMARAGYDYGAARDFWRQYGADFGRSRYAQWSHPGSLERATNLGATAEEIRGKQA
                     AGEPLVPTPARLTSAEQP"
     gene            complement(16091..16651)
                     /locus_tag="PLO65_00640"
     CDS             complement(16091..16651)
                     /locus_tag="PLO65_00640"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HRD26786.1"
                     /translation="MKIRNASVIVGLTVLAAAACSKADKAADANTAAAPAAAVEVPKR
                     KPGLWKQTMLVEEAGVLQTVQICLDAASDSKLAWWGQQGFKQACSKSEVTRQPDGSWK
                     FSSVCSGAGVKTTNDGSAVGDFSSKYQLKAASTTTGAPVPAMNGSRTITIDAEWIGEC
                     PAGMKPGDIDLANGQRVNMLEVSGQK"
     gene            16849..17664
                     /locus_tag="PLO65_00645"
     CDS             16849..17664
                     /locus_tag="PLO65_00645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRD26787.1"
                     /translation="MNLFSRLLGRKPARQGIALEGRRVFAVGDIHGCNDLLDGLVDAI
                     LREAEGDARPPLLIFLGDYVDRGPGSRQVIERLIALKDQAPGVRFLCGNHEEAMLHFL
                     SDLEAGMAWTGYGGKATMTSYGVTLPQDETSLDAWVETHDALKAAIPESHMRFLWGLE
                     DRIDLGDYLFVHAGVNPDRPLNAQNAKDLRWIREPFLSDGRRLEKVVVHGHTPGTAPH
                     CDARRIGIDTWAYRTGVLTALELDGPARRYLQARRDAAGAVTISTLAADDGQD"
     assembly_gap    17786..17901
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17902..>18667)
                     /locus_tag="PLO65_00650"
     CDS             complement(<17902..>18667)
                     /locus_tag="PLO65_00650"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRD26788.1"
                     /translation="RVQTRQAYVYATAARLGWAGPWLQAAEHGLDFFLRHYGRDDGLF
                     ATLASADGAIIDDTPMLYDQAFALLAMAAVHRANPGRRDVVDRARAHRDSLSLMRAEG
                     RGFRENGSWPYQANAQMHLLEAALAWFEATGDEAWGVLADEIVALALDAFIDPAGLFL
                     REVFDAAWRPAEGDDGRFIEPGHQFEWAWLLQTWSRLRPGRDVGPVIEGLMRTGARGV
                     DPRRDVAVDELWTDLSVRSHRARLWPQTERLRACLAR"
BASE COUNT         2968 a         6275 c         6359 g         2949 t
ORIGIN      
        1 ggcccggctg gcgcggcagg cctgcgccaa cctgggcggc gtgggcgtgg ccgaggtgga
       61 ggaggtgtgg cacgccctgg ccccgcccta cgacgacctg ttcgcctggc tcgaggggcg
      121 gtccgaccgg cccagcgccg atcatcctgg ccgcttccgg cctgtaatgc tggcccagcc
      181 cgtcgacgag gcggtcgact tcgcgcggct cgacccggcc gactacgccg ccgaatggaa
      241 gtgggacggc atccgcatcc aggcggtgcg cgagcgcggc gaggcgcggc tctatacccg
      301 caccggcgac gacatcagcc acgccttccc cgacgtgctg gcggcgatga gcttcgaggg
      361 ggcgctggac ggcgaactgc tgatcatgcg cgagggccgg gtcgcgccgt tcggcgacct
      421 gcagcagcgg ctgaaccgca agaccgtcga tgcgaagctg ctggccgccc atccggccgg
      481 catccgggcc tatgacctgc tgctcgacgg cgaggaagaa cttcgcgcgc tttcctttag
      541 ggaaagacga attcggctcg aatcgttcat cctggcggcg aatagccccc gaattgacct
      601 ctcgccgctg caaccgttcg aaacctggga gtcgctggcc gccctgcgcg cggcgccgcc
      661 ggagggcgat ccggccgtcg ccgaggggct gatgctcaag cgctgggaca gcccctacga
      721 ggcaggccgg ccgaaggggc cgtggttcaa gtggaaacga gacccgcacc tgatcgacgc
      781 ggtgctgatg tacgcccagc gcggccacgg caagcgctcg agcttctatt ccgacttcac
      841 cttcggcgtc tggcgcgagg acgccaacgg cgaccgcacc ctgaccccgg tcggcaaggc
      901 ctatttcggc ttcaccgacg aggaattgaa gcgtctcgac cggttcgtgc gcgacaacac
      961 gatcgaccgg ttcggacccg tgcggtcggt cagggcgacc cgcgatttcg gcctggtgct
     1021 ggaggtggcc ttcgagggcc tgcagcgctc gacccgccac aagagcggcg tggccatgcg
     1081 cttcccgagg atcagccgca tccgctggga caagccggcc cgcgaggcgg acgaactggc
     1141 gacgctggag cggatgctcg actgactacg gtgtgatcct ccccagcttg ctggggaggg
     1201 ggaccgccgg aggcggtgga ggggctgcgc ttacgtcagc gacccattct gaattcaggt
     1261 ctttccggag agttcgggtc agcccctcca tcatgctccg catggtcccc ctccccgcat
     1321 tgcggggagg attgatgtct acccgttgaa gcccttcacc gcgtcgatga tccgctgctg
     1381 ggtctcctcg tccatgtagg ggtgcatcgg cagggccagc accttgtcgg ccagggcctc
     1441 ggtcaccggc aggccgcccg cgccgcgcgg gtagtccgcg tacggcgcct gcacatgcat
     1501 cggcacggga tagtagaccg ccgtcggcac gccctgggtc ttcagatggg cggccaggcc
     1561 gtcgcggttc tcatgctcga tgacgtactg ggcccagacc gactggccgc cggcgatgac
     1621 cttcggcgtc gacagaaccg accctttcag accctcggcg tagcggtcgg ccaccccctg
     1681 ccgcatggcg atctcttcgg cgaagatggc cagcttctcg atcaggatcg cggcctggat
     1741 cgagtcgagt cgcgagttca tgccgatgcg ggcgttgaga tacttcgggt cgtggtcgaa
     1801 cacccggccg ttgaggtcct gggccaccgc cttgccgtgc acgcggtagc tgtccatctg
     1861 gtcccacagg gcctggtcct tgagcaacac cgccccgccg tcgccgtagc agccgagggg
     1921 tttggccggg aagaagctgg tggtggccac gtcggcccag tggatcggat ggtggccgtt
     1981 cagcgtgcag ccgaagccct gggccgagtc gctgatcagt ttcaggccat ggcggtcgca
     2041 gacggcccgt agggccgggt agtcggccgg ctggccgaac agatcgaccg cgatgaccac
     2101 ggcgggcttc agcttcccct cggccttcac cgcgacgatg gcggcctcga gcttcgccgg
     2161 gtcgaggttg aaagtgtcgg gcagcacatc gacgaacacc ggcgtggcgt cgacccaggg
     2221 cacgacttcc ggcgtggccg tgaaggtgaa ggacgggcag aagaccgcgt cgccggcgcc
     2281 gatcccccaa gccatcagcg gcagggcgat ggcgtcggtg ccgttggcgc agctcagcgc
     2341 cagcggcgcc tcgccgaagg cagccagctc ggcctcgaac tggcggacct cggggcccat
     2401 gacgtagccg ccatggtcga gaaccctggc gatggccgcg tcgaggcggc tccggatgcg
     2461 ccgctgctgc gcggcgaggt cgatgaaggc gatgctcatg cggcggctca tagcggcggt
     2521 ggggcggtgt ttcgacacaa accctgtaac cggttgttgc gtttgctagt gtcggcgcaa
     2581 atcgatcccg ccggaagcgc ccatgcaatt cgacgccgaa gttgatcaac gcctgcggca
     2641 gaccctgctc ggcgacgaat cgctgctgca caagttcacc gacgcggccg gcgacctggc
     2701 ggtcaacctg gtgatcggga cgattatcct cgtcctgacc ctgtgggcct cgtcctggtt
     2761 cgcgggactg acccgacgcg cgatgaaccg gcttcccggc cgccggacgc cggacctgac
     2821 gctgcagacc ttcgtcgcct cgatggtccg ctacgtgatc ctcgtggtcg gcttcgtggc
     2881 cgtcctgcag caactcggcg tcaagaccac gtcgatcgtc gcggtcctgg gcgcggcgtc
     2941 actcgccgtg ggcctggcgt tgcagggcgc cctgtccaac gtcgccgccg gagtgatgat
     3001 cctgctgttc cgcccgtacc gcgtcggcga tctcatcgag accggcggaa agaagggaat
     3061 cgtacgggct ctcgacctgg taatcacgga actggctacc gcggataatc tgaagatcgt
     3121 catccccaac aacaaggtgt tcggcgacgt catcgtcaat cacagccacc actcccagcg
     3181 gcgggtcgat gtgacgttcc gaatccccgc cgctgccgac atggcgggga tcatggccgg
     3241 cgtgctggcc agggcgaggg ccaatccgct ggtgctcgac gatccggagc ccagcgtcag
     3301 cgtgaccctg ctggccgaag ccgccgtcga gatcgaggtc ctggcctgga cccgttcggc
     3361 cgaccatgcc gcggtgcggg ccgacctgct gctgacggcc aagctgatcg aggcggggca
     3421 ggacgccagc ctgccgccgc tgccgaaggc cccgctcagc ccgccagcgc ctgcgaaagg
     3481 tccgccagca ggtcgtcgca gtcctcgatc ccgaccgaca gcctgatcag ggtgtcgctg
     3541 atgccgatcg ccgcccgctg ctcgggcggg atcgaggcgt gggtcatgat cgccggatgc
     3601 tcgatcaggc tctccacccc gcccagggac tcggccaggg tgaacagctg cgtccgttcc
     3661 agcacccgcc gggcccgcgc caggtcgccg gcgatctcga ccgagatgat gccgccgaag
     3721 ccgttcatct ggcgccgggc cagggcatgc tgcggatggc tctccaggcc cgggtagatc
     3781 acccgggtga cgtcgtcgcg ggcctcgagc cagcgggcga tgcgcagggc gctctggcag
     3841 tggcgctcca tgcgcagggc cagggtcttg aggccgcgca gggcgaggaa gctgtcgaac
     3901 ggtccggaca cggcgccgac ggcgttctgc aggaagcgca tctgctcctc gatggcctgg
     3961 ttctcgccga ccgccaccac gccgccgacc atgtccgagt ggccgttgag gtatttggtg
     4021 gtcgaatgca gaaccaggtc gaagccgtgc tcgagcggac gctggatcca cgggctggcg
     4081 aaggtgttgt cggcgaccgt gatcagcccg tgccgcttgc cgatcgccgc gacggccgcg
     4141 aggtcgacca gccgcagggt cggattggtc ggggtctcga cccagatcat cttcgtctcg
     4201 ggccggatcg ccgccgtgat cgccgcgggg tcggtcatgt cgacgaagct gaaggtcagc
     4261 ccggccgagc gcgcccgcac ccgctgcatc agccggtagc tgccgccgta gaggtcgtcc
     4321 gaggcgatca catgggcgcc ggcgtccagc agctccagcg tcgtcgaggc cgccgccagg
     4381 cccgaggcga aggcgaaggc cgcgaccccg ctctcgaggt tggcgacgca gcgctcgaag
     4441 gcgaagcggg tcgggttgtg gctgcgcgcg tactcgaagc ccttgtgcac gcccgggctt
     4501 tcctgggcga aggtcgaggt ggcgtagatc gggaccatga ccgccccggt ggtcggatca
     4561 tgcgcctggc cgccgtggat cgtgcgggtg gcgaaggcga ggcggttctt caacagttca
     4621 gtcatggtcc aaaccggttt ccctaatccg tcatggcccg acttgttcgg gccacccaag
     4681 cacgcgggct tgtccggata tggctgtgtt catgggtggc ccggacaagc cgggccatga
     4741 cggaaaagta gggtgttcag gccgtgcgtc tgaggtggtt gatgagatcg acgcgggtga
     4801 tcaggcccac cagcttctca ccgtccatca ccaccgcgac ctcgtcgcgg tcgaacagcg
     4861 gcttgagctc gtcgagcgac tgatggacct gcagggtgtt gacgtcgcgc gtcatggccg
     4921 aggcgacccg ggcgccgaaa cggtcggcgc ggccctcgcg accgccgacg gcggcgagga
     4981 tgtcgctctc gtcgaggatc ccgaccagcc ggccgtgatc gaccaccggc agctggctga
     5041 tgtcggccgc gcgcatgcgg ttgtaggccg tcagcaggct gtcgtccggc cccgtcgcga
     5101 tcacaccgcc ctcggagaag tcgcgcgcga tcaggtcgcg caggtcgccg gacctgggcc
     5161 ggtcctcgaa gccatgggcg gcgacccaca tgtcgttgaa catcttggtc aggtatttgc
     5221 cgccggtgtc gcagaccagg ctgacgaccc gctgcggggt cgtctgctcg cggcagtagc
     5281 gcagggcccc ggccagcagg gtgcccgacg aggagccggc caggatgccc tccttctcca
     5341 gcagcaggcg ggcggactcg acgctctcgc ggtcggggat ggcgtaggcc ttggccacca
     5401 ggtccatctg ggcgttgtcg gggatgaagt cctcgccgat gccctcgacg atccagctgc
     5461 ccgcctcgcc gtaggtcccg gtgttgacgt agtcgtagag gatcgagccg accgggtcgg
     5521 cgaggatcat cttcgtcttc ggcgagtgct ttttgaagaa ccggccaagg cccgtcaggg
     5581 tgccgcccga gccgacgccc acgaccaccg cgtcgacgtc gccgcccatc tgttcgagga
     5641 tctcggggcc cgtggtcatc tcgtgggcca gcgggttggc cgggttctcg aactggttga
     5701 cgaagaagcc gcccgtcgcc tgggcgatcg tctgggccat gtcctggtag tactccgggt
     5761 ggcccttgcc gacgtcggag cgggtgatcc gcacgtccac gcccatggcc cgaagatgca
     5821 ggatcttctc ccgcgccatc ttgtcgggca ccaccaggat cagcctgtag cccttgaccg
     5881 tggccacctg ggccaggccc aggccggtgt tgccggccgt cgcctcgacg atcgtgccgc
     5941 cgggcttgag ccggccgtcg gcctcggccg cctcgatcat cgcgcgggcg atgcggtcct
     6001 tgatcgaccc gcccgggttc tggttctcga gcttgaggaa cagccggcag acgccggtgt
     6061 cgatgtgcgt cacctcgacc atcggggtgt cgccaatcag gtccagcgcc gagcgcgtgg
     6121 tcgcgggaag ggtcatgaag ctcgctgccg caggggaaga gttgaccctg ccttagcgtg
     6181 gatcggggca tgacgaaagc cgcggcccgg ctatttcgga aagggcgtgc ccgtcagcag
     6241 agccagttcg tcggccacca gggcgtcgag atcgccgccc tcatagaaca cccccctgac
     6301 ccccgcccgt cgcgccgcct ccaggtcgga gggcttgtcg ccgatcatca ccgcatcggc
     6361 cggcacgacc gcgaaatcgg ccagggcgcg cagcagcatc cccggattgg gcttgcggtc
     6421 ggggtggtcc gcgacgcgat agcgatcctc ggtggcggcc tcgtggaagg ggcagaaata
     6481 gatggcgtcg atccgcgcgc ccgcggcggc cagctggcgc atcatctcgg cgtggaaggc
     6541 ccgcatgtcg gcctcggtgt agtagccgcg gccgatgccc gactggttgg tggcgatgac
     6601 cgcgacggct cccgcctcgt tgacgcggcg caccgcgccc cgggcccccg ggacccattc
     6661 cagcgcggcg ggatcgaatg cgtagccgtg gtccacgttg agcacgccat cgcggtcgaa
     6721 gatcaccatg acggggcgcg gcggactcat ggctccccca taccatccgc caccgaaagg
     6781 ggaagcgacc cgacatcacg gcggccgtcg cacgcaggca agagagatgc gcgtcgaccg
     6841 ttgttaaata acgatgctat tcgtaacatg cgcggcaaca gaataaaggg ggagaaggga
     6901 ccatgcgccg ctcttcatcg agatgggtcc cggacgttcc cgccatacgc gtttcgaagc
     6961 ggtggcttgt ccaacaggcg tcgagactcc ttggcgtggc ccttcttggc ctcttcccgt
     7021 tcgaccgggc ggccgcaaac aatgagtgtg gctttcctca ggcgcgagaa gtcacctgca
     7081 ccgccgagat gaacccgcac gcgaacggga ttcactatgc gacgcagaac aacctgacga
     7141 tcaacctggc aagcggcgtc atcgtaagga ccagcaaccc gacggcgaga ggcgtgcaga
     7201 tcattgccgg atcagcgtcc ggcgcgcgat tgtctatatt cggaggcccc ggggtatcta
     7261 tcgagaccag cggcgacagc gcaatcgggg tgctggcctt ttcgccgctc ggcagcgcca
     7321 ccgtccacgt cgactccgtc cgcaccagcg gcgacctgtc gaccggcgtg cacgtggtca
     7381 gcattctcac cgatgtgcgg gtgcacaacg tcaccacgac gggaaatgat gctcctggcg
     7441 tgttcgcccg gggacatgag agcgtcactg tggtcgtcgc ggacgcaata gacaccagcg
     7501 gggtcaactc gaggggcgtc atggccaccg ggcggatgac ggtcgaggtc gtcaacgcag
     7561 gtgacattag cacggccggc gccttttccg aggggatcta cgccgtttcc gacggaccgg
     7621 atcccggtgg gtcggtcgtc gtcaatagca ggaacacaag cgcccgtgac gctgtcgcca
     7681 tttacgccga gagcgcccat gggcccaccg ccctgacggt cacggggaca acgatctcgg
     7741 cgagacacga tgcgatcatc gccattggcg aaaccggagc gtctgtagcg gtggagagcg
     7801 gcgccaccgt tctgggtggc ctgaacgcca ttgtcatccg atccggaacg ggtgccgcca
     7861 cctcgccggc ggaggccgct ctcccggctc gttcaacggt gaccaatgcc ggggtcatca
     7921 ccgctggcga cggctttgcc atcctcgctt ccggcgcgcc ggtgcagatt cgaaatagcg
     7981 ggacgattaa cgggcgcatt tcgctgacgg acaacgacga cctggtggtc aatacgggga
     8041 ccttcaacgc cgaagggcgc agcgaattcg gcgctggcgc ggacgggttc cgaaacgacg
     8101 gagtccttcg cgcacagacc gccagcctcg tgaccttcgc cggagtcgaa tccttcagca
     8161 acagcggctt gattgacttg cgaaacgggc aggccggaaa cagcctcgtt cttacagggg
     8221 tcttcgtggg ctcgggccag agccagcttg ccctcgacgt cgtgcttgac gctacaggaa
     8281 gcgccagcga ccttctggtc gtgggcagcg cgaccggtga gactgaagtc ctgttggcag
     8341 tgtccggcgg gcccgcacgg ctcaatccag gacaactcct cattcagtct ggtgccgcct
     8401 cgtcaccaac ggcattcaga ctggccgaca gttcggctga tcagggcctg atccacttct
     8461 cgatcatcta cgacccgaca acgttcgact acagcctggt gggggtgccc ggcgcctcag
     8521 tctacaggat ggcaaaatat tccgagggcg cggccaacac ctggttggct gcatcagatg
     8581 cctggcgagc cggcctccag tcgcggcgcg acgggggagg tgacgggcct cgaacctggc
     8641 ttcaggtcca gggctccgag atcgaacggg atagcctctc ccgcaccacg gtgttcggcc
     8701 agacctcgtc gtttgatctc agctatggcc agaccagccg gggcgcccag tttggggcag
     8761 aagggtcgcc ggccgccatt ccggggctgt tgctgggcgt gaccggcggc ctgatcgact
     8821 cgcgggtgtc cttcaccaac tcccgcgaag aggctcgctt cgaaaccctg aacttcggcg
     8881 gctatgccag cttcagcgcc gggagtactt tcgtcgggat gctggccaag tatgactgga
     8941 gccggatcga cagcgatggc tgggggcagg gtccggcccg cagtatcgac ggcgaggcct
     9001 ggggcatcac cgtcgaagtc ggccatcgag ccggttcatc ggcgctgttt gtcgaaccgg
     9061 tcatcactgt cagctggctc ggcacaaggc tggaagactt taccgtacaa ggcagctcga
     9121 tcggatttga ggacgctgaa cacctgcgcg gaagtctggg tttcagaatg ggaggagacc
     9181 ttgggcccac ggtggccctc tacggaggcg ccgtcatggt cagggaattc gaaggcaagg
     9241 acggcctgac gtttcacgac agcacggcga ctttggagat cgccaacgag tcgatgccag
     9301 actacagcca gggacacatc ggcctgcggt tttcgcccgc cgccgccgtc agtgggttcg
     9361 ttgagggttt cgcaatcttc ggtggcgacg acggtgtggg gggtggtggc gggcgcgtcg
     9421 gtgtctccgc ccgattctga cggccagctc ccggcacctc gggggattga cagaattcct
     9481 tacaaggacg ccgaaatttc ggcaggtcag aaccgtcggc cataccgcca gtccacttgc
     9541 atcgaggcct cggcatccgt aactcccgga cccagacgaa ctgatagcgg cgtggagaat
     9601 gaccaatggc ggaatccgcg gacctggttc ccgacccttg taaccgcgcc gcgcttccga
     9661 ttcggatgac cgcatgactg ccctcgtttc ccccgcccgc ctgacgcacc tgcagcgcct
     9721 cgaggcggag agcatccaca tcatgcgcga ggtggtctcc gagaccgaac gcccggtgat
     9781 gctctactcg atcggcaagg acagcgcggt gatgctgcac ctggccgcca aggccttcta
     9841 tccgagcaag ccgccgttcc cgctgctgca cgtggacacg acctggaagt tccaggcgat
     9901 gtacgagatg cgagagcgca tggcccgcga gctcggcttc gacctgctgg tgcacaagaa
     9961 ccccgacgcc gaggcccgcg gcatcaatcc gttcgaccac ggcccggccc acaccgacct
    10021 gtggaagacc gagggcctca agcaggccct ggagaagcac ggcttcgacg cggccttcgg
    10081 cggcgcgcgc cgcgacgagg agaagagccg cgccaaggaa cgcatcttct ccttccgctc
    10141 ggccgagcag cgctgggacc ccaagaacca gcgaccggag ctctggcggc tctacaacgc
    10201 ccgcaagaac aggggcgaga gcatccgggt gttcccgatc tccaactgga ccgagctgga
    10261 catctggcaa tacatccacc tggagaacat ccccatcgtg ccgctgtact tttccggggt
    10321 gcggccggtg gtcgagcgcg acggcacgct gatcatggtc gacgacgacc ggttccggct
    10381 gcgcgacggc gagacgccga tgatgaagtc ggtgcgcttc cgcaccctgg gctgctaccc
    10441 gctgaccggg gccgtggaga gcaccgcctc caccctgccc gagatcatcc aggaaatgct
    10501 gctgaccacc accagcgagc gccagggccg ggtcatcgac cacgaccagg ccgcctcgat
    10561 ggagaagaag aagcaggaag gctatttctg atgagcagcg cgtacaaacc cgccgatctc
    10621 atcgagacgg acatcgacgc ctacctgctg caacaccagc acaagagcct gctgcggttc
    10681 atcacctgcg gcagcgtgga cgacggcaag tcgaccctga tcgggcggct gctctacgac
    10741 agcaagatga tcttcgagga tcagctggcg gcgctggagg ccgacagcag gaaggtcggc
    10801 acccagggcg gggcgatcga cttcgccctg ctggtcgacg gcctggccgc cgagcgggaa
    10861 cagggcatca ccatcgacgt ggcctaccgc ttcttcgcca ccgacagccg caagttcatc
    10921 gtcgccgaca cccccggcca cgagcagtac acgcgcaaca tggtcaccgg cgcctcgacg
    10981 gccgacgccg ccgtggtgct gatcgacgcg cgcaagggcg tgctgaccca gacccggcgg
    11041 cacagctacc tggtcaacct gctgggcatc cgccacatcg tgctggcggt gaacaagatg
    11101 gacctggttg gctgggacca gtcgatcttc gacgccatcg tcgaggaata cagcgccttc
    11161 gccgaccaga tcggcctggc cgacttcacc gccatcccga tgtcggcgct ggacgggcag
    11221 aacatcaccg agcccagcgc cgccgcgccc tggttcgacg gcccgccgct gatgcgctgg
    11281 ctggagaccg tcgcggtcga ggatgacctg ccatcgcgcg ccttccgcat gcccgtgcaa
    11341 tgggtcaacc ggcccaatct cgacttccgc ggcttcagcg gccagatcgc cgccggcacg
    11401 gtgaggccag gtgatcgggt caaggttctg ccctcgggcc gcgaaagccg cgtcgcgcgc
    11461 atcgtcgtgc tgccgggcga tctcgaccag gccgtggccg gccagtcggt gaccatcacc
    11521 ctggccgacg aggtcgatgt ctcgcgcggc gacgtgctgg tcggcgccga cgacccctgc
    11581 ccggtcgcca accagttcga atcgacactg gtgtggatgg acgacgaacc gatgctgccg
    11641 gggcggccct atctgatgaa gatcggcgcc cagacggtca ccgcctcgat caccgaaccc
    11701 aagtaccggg tgcaggtgaa cacgcttgag cacatcgccg ccaagcggct cgaactcaac
    11761 gagatcggcg tctgcaacct gtcgctcgac cgggccatcc cgttcgaggc ctatgcggac
    11821 aatcgcgacc tgggcggctt catcctgatc gaccggctga gcaaccgcac ggtcggggcc
    11881 ggcatgctgc atttcgcgct gcgccgcgcc gacaacatcc actggcaggc caccgacatc
    11941 gacaaggccg cccgcgccac ccagaagggc cagaagggcc gcgtggtctg gttcacgggc
    12001 ctgtcgggcg ccggcaagtc gaccatcgcc aacctggtcg agaagcggct gcatgcgctg
    12061 ggccgccaca cctacctgct cgatggcgac aacgttcgcc acggcctcaa caaggacctg
    12121 ggcttcaccg aggaggaccg cgtcgagaac atccggcggg tggccgaggt cagcaagctg
    12181 atggtcgacg ccgggctgat cgtgctgacc gccttcatca gtccgttccg cgccgagcgc
    12241 cgcatggccc gcgagatgct ggaggatggc gagttcgtcg aggtctacgt cgagacgccg
    12301 ctggccgtgg cggaacagcg cgacgtcaag ggcctctaca ggaaggcccg ggcgggcgag
    12361 ctgaagaact tcaccggcat cgacagtccc tacgaggagc cggagaacgc cgaactgacg
    12421 gtcgacacca cgaccctgtc gccggtcgag gcggccgaag cgatcgtcgc ctggttggag
    12481 aagtaggctc cgggcacatg cgcttcagtg cgtgtccccc agcagcttga agcggccggg
    12541 gacacgtgcc tggcggtaca tgtcccccct cacagcaccc cgttgtaccc gcggtaccag
    12601 tcgacgaagt tgcgcacgcc ctcctcgacc gtcacacgcg gcttgtagcc gacgatgcgc
    12661 tgcagttcgg tcacgtcggc caccgtgtcg ggcacgtcgc cgggctgaag aggcagcatt
    12721 tccatgatcg ccttgcggcc gaggcagtcc tcgagcacct cgatgtagcg cagcagctcg
    12781 acgttctctt ccgctccgat gttgaagatg cggtaggggc ccgagccgct ggtggccgga
    12841 tcgggattct ggccgtccca tagcgggttc ttcgccggca cattgtccat cgcccggatc
    12901 accccttcga cgatgtcgtc gatgtaggtg aagctgcggc tgtgcttgcc gtggttgaac
    12961 accttgatcg gctcgccggc caggatcgcc ttggtgaaca ggaacagggc catgtccggc
    13021 cggccccatg gtccgtacac ggtgaagaag cgcaggccgg tgcagggcag gtcgaacagg
    13081 ttggcgtagc tgtgcgccat ctgctcgttg gccttcttgg tcgccgcgta cagcgtcagc
    13141 ggatgctcgg tgctgtcgtg ctcggagaac ggcatcttcg tgttggcgcc gtagaccgaa
    13201 ctggtcgagg cgaacaccag atgttcgacg ccgttgtgcc ggcagccctc gaggatgtgc
    13261 aggaagccgg tgacgttgct ctgcacatag atgtgcgggt tggtcgccgc gtagcgcacg
    13321 ccggcctggg cggccaggtt gatgacccgc tgcggccggt gctcggcgaa ggcctgctcg
    13381 accgccgccc ggtcggccag gtcgatggtc agctgggtgt agtcgggatg gtcgatatgg
    13441 cgcgcgagcc gcgcccgctt gagggccgga tcatagtagt cgttgaggct gtcgaccccg
    13501 atcaccgtgt cgccccgggc cagcaggcgc agggtggtgg ccgaaccgat gaaaccggcg
    13561 ctgccggtga ccatgacctt catgggactg tccttcgtcg gtcagaggcg gccgtcgacc
    13621 gcgtcgcgcg gcagggccga cttgatgtcg tagagcacgc tcgccggctt gccgaaggcg
    13681 cggacgccga cggcgccaag gtcgacgaac tggcgatggg cgacggccag gatgaccacg
    13741 tcgtaggccg cccgggccgg gtcggccgtc agcgtcaggc cgtattcgtg ggccgcctcg
    13801 gcagggtcca cccaggggtc gtggacatcc accgccaggc tgtagtcgcg cagctcggcc
    13861 accaggtcga cgaccttggt gttgcgcagg tccgggcagt tctccttgaa cgccaggccc
    13921 aggaccagcg cccgcgcccc gaccggattg atcccgcggt gggccagcag cttgagcacc
    13981 tggccggcga cgtaggcgct catggcgtcg ttgatccgcc gcccggcgag gatgacttcc
    14041 ggatggtgcc cgatctcctg cgccttgtgg gtcaggtagt aggggtccac cccgatgcag
    14101 tggccgccga cgaggccggg ccggaacggc aggaagttcc acttggtgcc ggccgcctcg
    14161 agcacctcga gcgtgtcgat gcccagcttg ttgaagatca tcgcgaactc gttgatcagc
    14221 gcgatgttga gatcgcgctg ggtgttctcg atgaccttgg cggcctcggc caccttgatc
    14281 gagctggcca ggtgcgtgcc ggccggcacg atggcgcggt agagaccgtc gacgaacgcc
    14341 gccgcctcgg gcgtcgagcc ggccgtgacc ttggtgatgg tcgacagccg gtgctggcgg
    14401 tcgcccgggt tggcccgctc ggggctgtag ccgcagaaga agtcgacgtt gaacctgagg
    14461 cccgattccg cctcgaggat cggcacgcag acctcctcgg tgcagcccgg gtagaccgtc
    14521 gattcataga tcaccaccga gccgggcttg aggcaggccc cgatcgcccg gctggctccg
    14581 ctgatcgcgg tcaggtcggg ccggcgcgcc tcgtcgatcg gcgtcggcac cgtgacgatg
    14641 aacacgttgc ggtcctcgag gtcggccgga ctggcggaga accgcagccg cggcgccgcg
    14701 gccatcgcct cgggttcgac ctccagcgtg cggtcgtggc cgtcgcgcag ctcggcgatg
    14761 cgcccggcgt cgagatcgta gccgaccgtg tcgtgatgga cgccaaacgc caccgcaagc
    14821 gggagcccca catagccgag gcccaggacg cagatgcgtg gattcgtcag agaaagtgcg
    14881 tccaatcccg ttccttcacg gccatgatca atccggcgcc cgcttatagt cgggttggca
    14941 aacggacgcc aatctcgcgc ggtcggcgat tggagatgat caccgggtcg gactatggtc
    15001 gttctccctg cggcggagac ggacatggtg gcgagaccga cggcgatcct cttcctgaca
    15061 gcgctggccg gctgttccac cgtcgagccg gtggtcgccc tgccgtcggc cgaccgagac
    15121 gcgttgcggg ccgaggagac gtttcagcag gaggccagtc tcgaccggct gcgtcagcag
    15181 gacggacggg tggccgccgt gtcgttccgc ctgaacgtgg ccaacctcga cctctgcgcg
    15241 gacagggcgc tgctgaccgg catggtgctg catcacgccc tgcagtacgg ccccgacatc
    15301 cgcccggtgg cgatccgcca tttcggcctg accggccggc ccgcggtgct gaccgtggcg
    15361 ccggacagtc ctgcggcccg ggccgggatc caggtcggcg acagtctcgt ggccatcgac
    15421 ggatccgcct tcgccaacgc cggggaccgg ctggcgcggg atccgccgac ctatgacgag
    15481 acctcgaagg cccgcgccct gctcgacgga gccctggccg acggcgccgg cgtgctgacg
    15541 gtcgagcgcg acggtcgccc cctgaccctt gcgctcacgc ccgtcgccgg ctgcgcctac
    15601 gaggcgcagc tgactccgtc cgaggatgtc agcgcctggg ccgacggccg ccgggtggtg
    15661 atcaccacgg ccttcgtgcg ctatgcggcg acggacgacc agctggccgt gatcctcggc
    15721 catgagctgg gtcacaacgt gatgaagcac cgcgcgcgga tcgcgggcgg cggccccgcc
    15781 ggagcgctcc tgggcaatgc cggaacccag cgcgggggcc tgctgacggt cgagcgcgag
    15841 gcggactatg tcggcctcta cctgatggcc agggcgggct acgactacgg cgcggcgcgc
    15901 gacttctggc ggcagtacgg cgccgacttc ggtcgctcgc gctatgccca gtggtcgcac
    15961 ccgggctcgc tggagcgggc caccaacctc ggcgccacgg ccgaggaaat ccgcggcaag
    16021 caggcggccg gggaaccgct ggtccccacg cccgcccgcc tcacctcggc ggagcagccc
    16081 tgagcggcgg ctacttctgg ccgctgacct cgagcatgtt gacgcgctgg ccgttggcca
    16141 gatcgatgtc gcccggcttc atgccggccg ggcattcgcc gatccattcg gcgtcgatgg
    16201 tgatggtccg cgagccattc atcgccggga ccggcgcgcc ggtggtcgtc gaggccgcct
    16261 tgagctgata cttcgaactg aagtcgccaa cggccgaacc gtcgttggtc gtcttcaccc
    16321 cggcgcccga acagaccgaa ctgaacttcc acgagccgtc gggctggcgg gtgacctcgc
    16381 tcttgctgca agcctgcttg aagccctgct ggccccacca ggcgagcttg gaatcgctgg
    16441 ccgcgtcgag gcagatctgc acggtctgca gcacgccggc ctcttccacc agcatggtct
    16501 gcttccacag gccgggcttg cgcttgggga cctcgacggc ggcggcgggc gcggcggccg
    16561 tgttcgcatc ggcggccttg tcggccttcg agcaggccgc ggcggccagc accgtgaggc
    16621 cgacgatcac gctcgcattc cgtatcttca tgacagctcc ctgttccatc ggcgccgctc
    16681 gacgcgcgcg cccgatgtga cccgaaccct ccccgcgagg caaggggtct gcaaccggcg
    16741 cgcgccatcg ttgaccgagc cccgccacta tgaaaaagca gcggccagag ccattgtgcc
    16801 gcccatgtcc acagtcgccg ccttcgcgtc acgcccggga aattccgctt gaacctcttc
    16861 tctcggctcc tggggcgcaa gcccgcccgg caggggatcg cgctggaggg gcgcagggtc
    16921 ttcgccgttg gcgacatcca cggctgcaac gatctgctcg acggcctggt cgacgccatc
    16981 ctgcgcgagg ccgagggcga cgcgcggccg ccgttgctga tctttctcgg cgactatgtc
    17041 gatcgcggcc ccggttcacg gcaggtcatc gaacggctga tcgccctcaa ggaccaggcc
    17101 cccggcgtgc ggttcctgtg cgggaaccac gaggaggcga tgctgcactt cctcagcgac
    17161 ctcgaggcgg gcatggcctg gaccggctac ggcggcaagg cgaccatgac atcctacggg
    17221 gtgacgctgc cgcaggacga gacgagcctc gacgcctggg tggagacgca cgacgccctg
    17281 aaggccgcca ttccggagtc ccacatgcga ttcctgtggg gcctggaaga ccgcatcgac
    17341 ctgggcgact atttgttcgt gcacgccggg gtgaaccccg accggccgct gaacgcccag
    17401 aacgccaagg atctgcgctg gatccgggag ccgttcctgt cggacggccg gcgtctcgag
    17461 aaggtcgtcg ttcacggcca cacaccgggc acggcgccgc actgcgacgc gcggcggatc
    17521 ggcatcgaca cctgggccta ccgcaccggc gtgctcacgg cgctggagct cgacggcccc
    17581 gctcgtcgct acctgcaggc ccggcgtgac gcggcgggcg ccgtgacgat ctcgaccctc
    17641 gccgccgacg acggccagga ctgaccgtca gcgcggtcag cgaccggccc aggcctgcaa
    17701 cgccagaacg gcgcccagga tgtgatagag ggagctcgcg ggcgacggct cgtcgagaaa
    17761 cgcgccgtcg gcgtcctgct tgtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn ncgggccagg caggcgcgca gccgctcggt ctgcggccag
    17941 aggcgcgcgc ggtggctgcg caccgacaga tcggtccaca actcgtcgac cgccacgtcg
    18001 cggcgcggat ccacgccccg tgcgccggtc cgcatgagac cctcgatgac aggcccgaca
    18061 tcacggccgg gccgcagccg cgaccaggtc tgcaacagcc aggcccattc gaactggtga
    18121 ccgggctcga tgaaccggcc gtcatccccc tcggccggcc gccaggccgc gtcgaacacc
    18181 tcccgcagga acaacccggc cgggtcgatg aaggcgtcca gcgccagggc gacgatctcg
    18241 tcggccagca cgccccaggc ctcgtcgccg gtggcctcga accaggccag cgccgcctcg
    18301 agcagatgca tctgggcgtt ggcctggtag ggccaggagc cgttctcgcg aaagccgcgg
    18361 ccctcggccc gcatcaggga caggctgtcc cgatgcgccc gcgcccggtc gacaacgtcg
    18421 cgcctgccgg gattggcgcg atgcacggcg gccatggcca gcagggcgaa ggcctggtca
    18481 tagagcatcg gggtgtcatc gatgatcgcc ccgtcagcgg acgccagcgt cgcgaacaga
    18541 ccatcgtcgc gcccatagtg gcgcaggaag aagtccagtc cgtgctcggc ggcctgcagc
    18601 cagggtcccg cccagccgag cctggcggcg gtggcgtaga cgtaggcctg ccgcgtctgc
    18661 acccggg
//
LOCUS       DAORNX010000007        17775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2414_length_17775_cov_7.842777, whole genome shotgun sequence.
ACCESSION   DAORNX010000007 DAORNX010000000
VERSION     DAORNX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17775
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_17775_cov_7.842777"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLO65_00655"
     CDS             <1..1103
                     /locus_tag="PLO65_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRD26789.1"
                     /translation="HQTVPQIGAMYRGDYRRKWTLAEYGFRLPSALDNRPLKFEEWEA
                     MRPQTVHVSATPGPWEMDKAGGVFAEQVIRPTGLIDPPVEVRPVSKDNASQVDDVIAE
                     ARDCAARGYRTLVTVLTKKMAEDLTEYMHEQGLRVRYMHSDVDTLERIEIIRDLRLGA
                     FDILVGINLLREGLDIPECGLVAILDADKEGFLRSETSLIQTIGRAARNVDGKVILYA
                     DRITGSMERAMGETSRRRDKQQAWNAANGITPESVKSRIKEILASPYERGDRVNVDVG
                     VAEDARPFLGSNFQATLRDLETKMREAAGNLEFETAARLRDEIKRLKMMDLEFANDLM
                     TAEGETADKAAPMRERKAARAEAAERFRKGRL"
     gene            1177..3147
                     /locus_tag="PLO65_00660"
     CDS             1177..3147
                     /locus_tag="PLO65_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD26790.1"
                     /translation="MNEDVEIQDFDPLSESLELPGADNSIFQEATRRVVENILKSYTG
                     YFDAFSEMIQNALDAIDARRRQDGDSYHPALWIDVDIRGRRLRVTDNGIGMDRAQFLY
                     CFRPNVSFKSRRESRGHKGVGATFLAYGYSVATLHSKRDGQTHSARLRNGRQWADDQG
                     GHYDRPKLEQIPFLVPELEASPSGTSIEIVIGDGQRPNLAWLNATRATQWSQVLRSRT
                     PLGGVYLKAPEKVTRTKVFVRVTDAGGNTSEETLDNPEYMYPHEIEFLQRVQSIRSIQ
                     TELAKLKGDPQEQMRVIPEQFKRLDASWEVWDKFDILDEDSPLSRNLDTGQRELIERH
                     NVVVYACFVSTAKTWNKWRDEVLQINKVADVMKGGLVIASDHMVQGDRLVIPLTSAIG
                     YQANTHIIVHFSDGNPDMGRKAFQPELKTLAEELAKRVVDIFKKYISLMREDAGNPIQ
                     SASRELQQWLLAQEQWRSQNPLDYKDGDLRLAFLSSPRWEQDVVALFHELIGMGVIRG
                     YGIYATSEHFRYDSLILTDYPDQTYAFDLTSNRLGVGLPHIGIGESGAMILEYKYNMD
                     GLIADFDKEEKYPRDVKLCVCWELGDNYKEHYSVESLLVGTNGGDRIFYGSTHKVYKD
                     RTPEFEVVCLHDLMKFLLNPQDEEARQKALYN"
     gene            complement(3319..3540)
                     /locus_tag="PLO65_00665"
     CDS             complement(3319..3540)
                     /locus_tag="PLO65_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD26791.1"
                     /translation="MAANEDLIALGREIRRHRKALNLSQEALAERAGLHRNYIGFLER
                     GERNPSASTVFLLARVLGVSTAELVAGIG"
     gene            3621..4043
                     /locus_tag="PLO65_00670"
     CDS             3621..4043
                     /locus_tag="PLO65_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26792.1"
                     /translation="MARAGKPTDTRIRPWLLLAPLPLTVGAAAAFAWLRIDPPLLVAL
                     WLVLGSVSAGIVARAWDAITEETPLPTFTVLSRVDAYADYVAQVEADSPEEAVDLAYD
                     GDPSVKWEPLGVVEFDARRVVALSADGEEIESTARGKG"
     gene            4071..4868
                     /locus_tag="PLO65_00675"
     CDS             4071..4868
                     /locus_tag="PLO65_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HRD26793.1"
                     /translation="MAGGEDDSWSNLGLAEERAVFVSPSQNARVLSEGWIAAHMFCPS
                     CGADRLEAFPNNSPVADFGCGACGEEFELKAKNGRLGRKVVDGAYGAMTARLKQRNNP
                     SLLAMAYDRARMRVSDLIVVPNHFFTQAIIEPRKPLGPHCRRAGWQGCNILIGDVPVA
                     GRISLIRDSIQVPKAAILAQWQATLFLRDSGLAARGWLLEVMKAVEDIGRAEFTLDDV
                     YAYEGRLSALYPGNNNVRPKIRQQLQVLRDRGFLEFGGRGVYRRVVH"
     gene            5040..5612
                     /locus_tag="PLO65_00680"
     CDS             5040..5612
                     /locus_tag="PLO65_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26794.1"
                     /translation="MTKTPLMGKATGEKSARDAAAELRWAWELHKEADNLLHQRLNGF
                     VAVNAFLAAGYFFATEAAYNGKPAHSFVLGISVAGVFLGPVFLGIIRRLVRGIGHLKQ
                     EHLFHDEIYSLYFGKRSKGPFVYWFAYGVPGILTLLWLAALGYSLLPIGPTLAVAAPP
                     SCVVAASACPPPQPLKTSTSLPKSLPTPSK"
     gene            complement(5809..6024)
                     /locus_tag="PLO65_00685"
     CDS             complement(5809..6024)
                     /locus_tag="PLO65_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HRD26795.1"
                     /translation="MSFQAYLDNIQAKTGKSPADFRALAAEKGFADAAGLRPGVKAGQ
                     IVQWLKDDFALGHGHAMAIVALLKGKT"
     gene            6207..7724
                     /locus_tag="PLO65_00690"
     CDS             6207..7724
                     /locus_tag="PLO65_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26796.1"
                     /translation="MTQAGAPSGANVGEAVIAPPGLGPRPKVGVPTKLLYGFGSVAFG
                     VKDNGFRVFLVFYYNQVVGLPVMWVTVALAIALAVDSFVDPVVGQMSDYWKSKWGRRH
                     PFLYASAIPAALSFLLLWNPPFHLSHGWQLAWLVGTAIIVRTFITCFEIPSSAMTAEL
                     TEDYDERTKIIGYRVLMAWWGGLTLNVFALLVLMRPTEGFKDGRLNPDSYGPYGWIAA
                     GLIFASIMLSAIGTHRFIPWMPKGPTTRPPGLIQLFKQMAGTLKNKAFLTVAGAALFA
                     AAAEGVGFSIYIYLVTYFWKLTAMQMGILITDAYFGSLVAFFFAPFISKGRNKKGAAM
                     ITLVLTVLLSALPILLRLGGLFPPEGSPWVIPALYGIAIIRGALGITCSILIVAMIAD
                     VVEDSQVQTGRRSEGLFFAAVSFISKSVSGVGAIITGLILTLIHFPVGQPASAVPQAT
                     INALGLAYIPTLFVLYGAALFCLSFYRITRESHAANLEKIAAMARAEEEAMPPPL"
     gene            complement(7705..8952)
                     /locus_tag="PLO65_00695"
     CDS             complement(7705..8952)
                     /locus_tag="PLO65_00695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD26797.1"
                     /translation="MTKPESLGLSSERLARIAPFIEARYIQSGKLPCAQVQVWRRGKL
                     AYDTVLGLADRERGTPLAADSLFRIYSMTKPVTSVAFMMLVEEGLVALDEPVAKYIPE
                     WANLGVFAAGTDAGWATTPPARPMLVIDLLRHTSGLTYGFQMQGNIDAAYRKLKVAED
                     LRFGTLDEFIAKLATIPLQFSPGDAWNYSVSTDVLGYLVGRISGIPFERFLQERIFGP
                     LGMTDTSFTVPADKAHRLTACYAAGVLGSKAVAAGPPKLQDDPVKSPYLTETGFHSGG
                     GGLVSTADDYMRFARMLLNGGELDGQRLLGPKTLQLMTANHLPGGKDLTQMSKSLFSE
                     ATYAGVGFGLGFGVTIDPAATMLAGSKGDFFWGGAASTFFWVDPVEDLAVVFLTQLLP
                     SSAYPVRRELRTLVYAALTEAAA"
     gene            9058..10971
                     /locus_tag="PLO65_00700"
     CDS             9058..10971
                     /locus_tag="PLO65_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD26798.1"
                     /translation="MAATTGANAPSELDIEAARWFFQNSVDVFVLIRGGEISRINPAW
                     TELSGWSVEETVGRPIEDFIHPGDAQIVLDLVSTLIADGQGRAEHRLLRKDGEILWVK
                     SRVKRAAGDATIVVLEDVTDEHRRRADSADAARTNDLLREAAGVYIWRFDPQTGLYDI
                     DTDLSKAGAPGASGSRTITADEMTSEIHPDDQARVGEAFFHTLATGEPRVVEYRHFRA
                     EDGGWARLRAAWRGLRRRESGKWDVLGLTQDVSEVAEARDAALNAAEAKAQFLANMSH
                     EIRTPMNGVLGVLHLLKNENLSPDGRQLLEEAVGCGQMLAELLNDVIDFSKIEAGRLE
                     FSPEPIDPAVMLDSVAGLLRPQAVARGLWLNTHVHPDLGWAMVDPVRLRQILFNLIGN
                     AVKFTLNGGVDARLSRVERDGVSRLRFEISDTGVGIGPDARADLFQRFHQADGSTTRR
                     FGGSGLGLAISRRLAELMGGEIGYDSQLGEGSTFWFEIAAPPVEARATESGAGDGLLE
                     GLRVLVVEDNPTNRLIATRMLQQLGAQVETADDGGQGVEAVHRSAFDLIFMDIQMPVM
                     DGLEATRRIRAMAEPVRSTPILAMTANAMSHQAESYLAAGMNGIVAKPLSPAALVAQI
                     AAVLATAARVAAA"
     gene            11075..12808
                     /locus_tag="PLO65_00705"
     CDS             11075..12808
                     /locus_tag="PLO65_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRD26799.1"
                     /translation="MAWPARSIADCHAILTAPGSRFETEDVVIDGVPTKVWKNGPATL
                     RDLVTNGRAFARRDFLVYEGDRATFEGFHRAVAALAERLAADGVRKGDRVALVMRNLP
                     EWPVAFFAAASLGAIVTPLNAWWTGAELQFGLADSGARIAIVDPERLDRILDHLPDCP
                     AIERLYVCRHAGAIDDARVHRLESVIGPVGAWETLPDRPLPVTPIAPDDPATIFYTSG
                     TTGRPKGALGTHRTMTTNVMTTGAALARAFLRRGETPPVPSPDAPQRVTLLVVPMFHV
                     TGCSATLCGALNNGGRLVLMRKWDALEGMQLIEREKVTATGGVPTIAWQLIEHPRRSE
                     FDLSSIESISYGGAPSAPELVRRIKEVWPSASAGNGWGMTETSATFTSNNAEDYERRP
                     DSCGVAPAVGEMKIMSPDGARELPAGEVGELWCKGPMVLRSYWNRPDATAETFVDGWV
                     RTGDLARLDEEGFCFIIDRAKDMLIRGGENIYCIEVENVLYDHPAVMDAALVGIPHRT
                     LGEEPGAVVTLKPGADASEQELRAFVLERLAAFKAPVRVAFWPETLPRNANGKIMKSE
                     LRSIFIQDVVA"
     gene            12805..14043
                     /locus_tag="PLO65_00710"
     CDS             12805..14043
                     /locus_tag="PLO65_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD26800.1"
                     /translation="MTVEAVLPRPSATARYAAKKEAILGAATTILNRQGVKGMTLADV
                     AAEVGLITTSVTYYYRRKEDLAAACFMRGIDMFNGLAAEAATVAGARARLARFLELFF
                     DLSHRIRTRVTAPIVAFGEMRALKEPLRTQVSDAFNDLFRTIRGFFDDPEFAGLDRRE
                     ATARTHLLLEQAFWTVTWLRRYDIEDYPRLRDRMFDILSGGLAVGARAWAPPSLPEAA
                     PLESGRHDISHETFLVAATRLINRKGYRGASVEEISAELNVTKGSFYHHNEAKDDLVA
                     ECFERTFVVMRDIQFAARDLKVDQWTRLAAASAALVEYQLSDRGPLLRASAMSALPTD
                     LRHETVERYNRVTDRFAAMISDGIAEGSIRPVDPLVAAHMLSSMLNAAASLGQWAPGL
                     KPEEAAALFARPLLLGVFTR"
     gene            complement(14221..17157)
                     /locus_tag="PLO65_00715"
     CDS             complement(14221..17157)
                     /locus_tag="PLO65_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26801.1"
                     /translation="MRKIGYLAGGSALAIALSLGAASTASAQATQPTEVEAVVITGSL
                     IAGTPEDAAMPVDVIGAEELSKQGSPSTVELLRALPVSSGILGETNQFDARAQGSEGS
                     GSVNLRGLGSQRTLVLMNGRRITPNPFGQVGTGIVDTNIIPSAAIGRVEVLKDGAAAT
                     YGSDAIAGVVNFITRKNFEGLEVAGSYKSVEGSDGDYTGSAVWGWVGDSANILLTAGF
                     QHRSELPVTERDWANLDFASNPQGGWTGGGGMAFLPTFSPAPGIYAPAAGLQTDVGCA
                     PLGGRATAGGCGFHYTPYDNITEIEDRYQLYGEVNIDVTDTTTFHGELLYAHTDVPEW
                     KTSPSYLALQSPTTATSPAPRVANLAGYWVPSSNPGFIAYQAANPAQLPAFANGAFFP
                     GVLYRPFAMGGNPMFGDGPSEGSREFEAVRINGELTGEFGNGIGWNVALTYSQETGER
                     TGYDTVVPFFQMALRGYGSLAGDASGCTAAETANYTTNAGNNALGCYYFNPFSNAVQS
                     NSITGATNPGYNSAVANNVDLVRWFFRKLSTKQTQRLFVADGLINGKLNWDLGGGQPA
                     WALGVQYRREYFISDYNNISDGNVNPCIDTPFNGSTACTVRNGAFFFLGAGFESDLQG
                     DVYATFGELQLPFTDDFNVQLAARYEDYGGEVGSTFNPKIAARWQVNDWLALRGSAGT
                     TFRGPPQTNLAGGFVTSLQFIAGSFRAIDIYGNPNLEPETANTYSAGLIVKAGGFKAS
                     LDYWRFDFDNPIVAEPSGDIVATMFPAALGTTGHCGDPAYAGLEARFTFGGACSTTNI
                     SRLRTLTVNGAAVKTSGLDLLADWRFDEFLGGSFTVGTSLTYTFEYKTDALAVGGVTV
                     APAFDAVGQLNYQTTAYPLPQMKGDVHFEYTNGDHNLRLTVRYVDSYRDQRAAITGAG
                     KEIDAYATGDLAYRVFLPWDTTFSAAVENITDQDPPLARLDLNYDPFTASALGRTIKF
                     NVVKKF"
BASE COUNT         2990 a         5912 c         5904 g         2969 t
ORIGIN      
        1 gccaccagac ggtgccgcag atcggggcca tgtaccgcgg cgactaccgc cgcaaatgga
       61 ccctggccga gtacggcttc cgcctgccca gcgcgctcga caaccgcccg ctcaagttcg
      121 aggagtggga ggccatgcgg ccgcagacgg tgcatgtctc ggccaccccc ggcccctggg
      181 agatggacaa ggccggcggc gtgttcgccg aacaggtcat ccgccccacc ggcctgatcg
      241 acccgccggt ggaggtgcgc ccggtctcaa aggacaacgc cagccaggtc gacgacgtca
      301 tcgccgaggc ccgcgactgc gccgcccgcg gctaccgcac cctggtcacg gtcctgacca
      361 agaagatggc cgaggacctg accgaataca tgcacgagca gggcctgcgc gtgcgctaca
      421 tgcacagcga cgtcgacacc ctcgagcgca tcgagatcat ccgcgacctg cgcctgggcg
      481 ccttcgacat cctcgtcggc atcaacctgc tgcgcgaggg cctcgacatc cccgagtgcg
      541 gcctggtggc catcctcgac gccgacaagg agggcttcct ccgctcggaa accagtctga
      601 tccagaccat cggccgcgcc gcccgcaacg tcgacggcaa ggtgatcctc tacgccgacc
      661 gcatcaccgg cagcatggag cgcgccatgg gcgagaccag ccgccgccgc gacaagcagc
      721 aggcctggaa cgccgccaac ggcatcacgc ccgagagcgt caagagccgc atcaaggaga
      781 tcctcgcgag cccgtacgag cggggggacc gggtcaacgt cgacgtcggc gtcgccgagg
      841 acgcccggcc gttcctgggc agcaacttcc aggcgacgct gcgcgacctc gaaacgaaga
      901 tgcgcgaggc ggccggcaac ctggaattcg aaaccgccgc gcgactgcgc gacgagatca
      961 aacgcctgaa gatgatggac ctggaattcg ccaacgacct gatgaccgcc gagggcgaga
     1021 cggcggacaa ggccgcgccg atgcgggagc ggaaggcggc gcgggcggag gcggcggagc
     1081 ggttcaggaa ggggcggttg tagggccagc gcgaggttgc taactgcgca atcttaggtg
     1141 caacctgttc gttcattgcg gcacgagggg ggcaacgtga acgaagacgt cgagatccag
     1201 gattttgatc cgctttccga gtccctggaa cttcctgggg cagacaactc gatttttcag
     1261 gaagcaacac gacgcgtagt tgagaacatc ctcaagagct acacgggata cttcgacgcc
     1321 ttttcagaaa tgatccaaaa tgctctcgac gccatcgatg ctcggcggcg gcaggatggc
     1381 gatagctacc atccggctct ctggattgac gtcgacattc gcgggcgtcg tctgcgagtt
     1441 acggataatg gaattgggat ggatcgcgcc cagtttctat actgctttcg tcccaatgtg
     1501 agcttcaaga gccgccgcga gagtcgcggt cacaagggag tcggggcgac gtttctcgct
     1561 tacgggtatt cggtggcgac acttcattcc aagagagacg gacagacgca ttccgcacgc
     1621 ctgaggaacg gccggcaatg ggccgacgac caaggggggc actatgaccg ccctaaactc
     1681 gagcagattc cctttctagt tccagagctt gaggcttcgc cgagcggcac gtcgatcgag
     1741 attgtcatag gcgatggcca aagacccaac ctcgcgtggc taaacgcaac tcgtgcgaca
     1801 caatggtctc aggtccttcg ttcgcgcacg cctcttggag gagtatatct taaggctcca
     1861 gaaaaggtaa cacgcacgaa ggtattcgtg cgtgttacag acgcaggagg gaataccagc
     1921 gaagaaactc tcgataatcc ggaatacatg tatcctcacg aaatcgagtt cctccaacgg
     1981 gtccagtcaa ttcgatcaat tcaaacggag cttgctaaat taaaaggaga tccgcaagag
     2041 cagatgcggg taattccaga acagttcaaa cgcctagacg cctcttggga ggtatgggat
     2101 aaatttgata ttctagatga agattcccca ctgtcccgca atctcgatac gggtcagagg
     2161 gaacttattg agcgtcacaa tgtggttgtg tatgcatgct tcgtttcaac tgccaagacg
     2221 tggaacaaat ggcgagatga agttctacag ataaataagg tcgccgacgt tatgaaaggc
     2281 ggccttgtta tcgcctctga tcacatggtt cagggtgatc gtctcgtcat tccgctaacg
     2341 tcggcaattg gttaccaggc aaatacacat atcattgtgc atttttccga tggaaatccg
     2401 gatatgggcc gcaaggcctt ccaacccgag cttaaaacgc ttgcggaaga gttggcaaag
     2461 cgagttgtcg acatcttcaa gaaatacatc tctctgatga gagaggatgc gggaaatccc
     2521 atccagtcgg cgtcgaggga gcttcagcag tggctacttg cgcaagaaca atggcgctct
     2581 caaaatcccc tcgattacaa ggatggggac ctacgtcttg cgtttctctc gtctcctcgt
     2641 tgggagcagg atgtcgtggc gcttttccac gaactgatcg ggatgggtgt aattcgcggc
     2701 tacggaatct acgctacgag tgagcatttc cgatatgact cattgatact gaccgactac
     2761 ccagatcaaa cttatgcatt tgacctcaca tcaaacagat tgggggttgg gcttccgcat
     2821 attggcattg gcgagtccgg tgcaatgatt ttagaataca aatacaatat ggacggattg
     2881 atcgccgatt tcgacaagga agaaaagtac cctagagacg ttaaactatg cgtatgctgg
     2941 gagcttggcg ataattataa agaacactat tcagttgaaa gccttttggt gggcacaaat
     3001 ggcggcgata gaatatttta tggctcaact cacaaggttt acaaagacag aacacccgaa
     3061 ttcgaagttg tttgcctaca cgacttgatg aagttcctct tgaatccgca ggacgaagag
     3121 gcgagacaga aggccctata taattaggca tccctccatc ttatgccgag acgatcgccg
     3181 ggcggtccag gttgctcgag ggtggccctt cgggacatcg ctccgcgacg cgaagcgccc
     3241 ttgtccaacc tggaccgtcc ggcacgctgg cctgcgtgag atcaaggaac agcccccttc
     3301 cccctggagg gggaggactc acccgatccc cgccaccaac tccgccgtcg acacccccag
     3361 cacccgcgcc agcaggaaca ccgtcgaggc gctgggattg cgctccccgc gctccagaaa
     3421 gccgatgtag ttgcggtgca ggccggcacg ctcggccagg gcttcctggc tgaggttcag
     3481 cgccttgcga tggcgtcgga tttcgcgccc cagggcgatc aggtcttcgt tcgcggccac
     3541 gcgcgaaggt tgccttgacg ccgagtccgc gctacatact gtcagtgtgt atttcgacca
     3601 cggcacgagg cggcgcccgg atggcgcgag caggcaaacc cactgacacg cgcatacggc
     3661 catggctgct gctggcgccg ttgccgctga ccgtcggagc cgccgcggca ttcgcctggt
     3721 tgcggatcga ccctccgctg ttggtggcgc tctggctggt gctgggctcc gtgtcggcgg
     3781 ggatcgtcgc gcgggcctgg gacgccatca ccgaggagac gccgttgccc accttcaccg
     3841 ttctgagccg cgtcgacgcc tatgccgact acgtcgccca agtcgaggcg gactcgcccg
     3901 aggaggccgt cgacctggcc tacgacggcg atccgtcggt gaagtgggag cccctgggcg
     3961 tggtcgaatt cgacgcccgc cgggtcgtcg cgctcagcgc cgacggcgag gagatagagt
     4021 ccaccgcgcg cggcaagggc tgagccacca agcatgctac agtcccgccg atggctggtg
     4081 gcgaagacga ttcctggagc aacctcggcc ttgccgagga gcgggcggtg ttcgtcagcc
     4141 cgtcccagaa cgcccgcgtc ctgtcagagg gctggatcgc cgcgcacatg ttctgtccga
     4201 gctgcggcgc ggatcggctc gaggccttcc ccaacaactc gcccgtggcc gacttcggat
     4261 gcggcgcctg tggcgaggag ttcgagctca aggccaagaa cggacgactg ggtcgcaagg
     4321 tggtggacgg cgcctacggc gcgatgaccg cgcggctgaa gcagcgcaac aaccccagcc
     4381 tgctggccat ggcctacgac cgggcccgga tgcgcgtcag cgacctgatc gtcgtgccca
     4441 accacttctt cacccaggcg atcatcgaac cgcgcaagcc gctgggcccc cactgccgga
     4501 gggcaggttg gcagggctgc aacatcctca tcggcgatgt ccccgtcgcg ggccggatca
     4561 gcttgatccg cgacagcatc caggtcccca aggcggccat tctcgcccaa tggcaggcga
     4621 cgctgttcct gcgcgactcg ggcctcgccg cgcgcggctg gctgctggaa gtgatgaagg
     4681 cggtcgagga tatcggccgg gccgagttca ccctcgatga cgtctacgcc tacgagggcc
     4741 ggctctcggc gctctatccc ggcaacaaca acgtccgtcc gaaaatccgc cagcaattgc
     4801 aggtgctgcg cgaccggggc tttctcgaat tcggtggtcg gggcgtctat cggcgggttg
     4861 tccactaacc cacaccaccc ccttcatctc atgccgagac gatcgccggg cgggccaggt
     4921 tgctcaaggg cgctgcgcgt cgcggagcga caggcccgca gggcctgccc ttgaccaacc
     4981 tggaccgtcc ggcatgctgg cttgcaagag atcgggcggc atctcgtcgg aactacggca
     5041 tgaccaagac cccattgatg ggcaaggcga ctggggagaa gagcgcaaga gacgctgcgg
     5101 cggaacttcg ctgggcctgg gaactccaca aggaggctga caaccttctt caccagcggc
     5161 tcaacggctt cgtcgcggtc aatgccttcc tcgccgccgg atacttcttc gccacggaag
     5221 ccgcttataa cggcaaaccc gcccacagct ttgtgctcgg gatcagcgtg gccggcgtgt
     5281 ttctggggcc ggtgttcctc ggcatcatcc gccgcttggt gcgcggcatc ggacatctga
     5341 agcaggagca cctcttccac gacgaaatct acagcctcta tttcggcaag cgttcgaagg
     5401 ggccgttcgt ctactggttc gcttacggcg tacccggaat ccttacgctc ctatggctgg
     5461 cggcgttggg ttattccttg cttccgatcg gccctacgct cgctgtcgcg gctccgccca
     5521 gctgcgttgt agcagcatcg gcatgtccac cgccccagcc gctcaagaca agcacatctt
     5581 tgcccaagag cctgccgacg ccgtcaaagt aagggccggg gataggggtg ctaccccggg
     5641 gatttcggtg acagtgcact tatctcccac cgcgctcctc ccatgcggcc acgcgatcgg
     5701 ctggaaataa gtgcactgtc accgaaatcc gggacgtccc ggccacgccg cgcatccctc
     5761 tccaccagcc tccggctggt ccccctctcc caagtgggag aggattggct acgtcttccc
     5821 cttcagcagc gccacgatgg ccatcgcgtg cccatgcccc agggcgaagt cgtccttgag
     5881 ccactggacg atctggccgg ccttgacgcc ggggcgcagg ccggcggcgt cggcgaagcc
     5941 cttttcggcg gccagcgcgc ggaagtcggc ggggctcttg ccggtcttcg cctggatatt
     6001 gtcgaggtag gcctggaagc tcatggcggc atgatcgccc ggcggggcgg ggggtcaagc
     6061 cttccgctcc agcgttccgc ctccccgccg cggggcggaa ttccctgttc gcggccgccg
     6121 gccgcgattg acttccccta gggccatacc tcaccttcga atccgttcag cgacggcaag
     6181 atcgcggacg accgggagga gactggatga cgcaggccgg ggcgccgtcg ggcgcgaacg
     6241 tgggcgaggc ggtgatcgcg ccgccgggac tgggcccgcg cccgaaggtc ggggtgccga
     6301 ccaagctgct ctacggcttc ggctcggtgg ccttcggcgt caaggacaac ggcttccgcg
     6361 tcttcctcgt gttctactac aaccaggtgg tcggcctgcc ggtgatgtgg gtcaccgtgg
     6421 ccctggccat cgcgctggcc gtcgacagct tcgtcgaccc ggtggtcggc cagatgagcg
     6481 actactggaa atcgaagtgg ggccggcgcc acccgttcct ctacgcctcg gcgatcccgg
     6541 cggcgctgtc gttcctgctg ctgtggaacc cgccgttcca cctgtcgcac ggctggcagc
     6601 tggcctggct ggtcggcacg gcgatcatcg tgcgcacctt catcacctgt ttcgagatcc
     6661 cctcctcggc catgaccgcc gagctgaccg aggactacga cgagcggacc aagatcatcg
     6721 gctatcgcgt gctgatggcc tggtggggcg ggctgacgct gaacgtcttc gccttgctgg
     6781 tgctgatgcg gccgaccgag gggttcaagg acggccggct caaccccgac agctacggcc
     6841 cctacggctg gatcgccgcc gggctgatct tcgcctcgat catgctgtcg gcgatcggca
     6901 cgcaccgctt catcccctgg atgcccaagg ggccgacaac ccgcccgccg gggctgatcc
     6961 agctgttcaa acagatggcc ggcacgctga agaacaaggc cttcctgacc gtcgccggcg
     7021 cggccctgtt cgccgccgcg gccgagggcg tcggcttctc gatctacatc tacctggtga
     7081 cctacttctg gaagctgacg gcgatgcaga tgggcatcct gatcaccgac gcctatttcg
     7141 gctcgctggt cgccttcttc ttcgccccgt tcatctccaa gggccgcaac aagaagggcg
     7201 cggcgatgat caccctggtc ctgaccgtgt tgctgtcggc cctgccgatc ctgctgcggc
     7261 tgggcgggct gttcccgccc gagggctcgc cctgggtgat cccggccctc tacggcatcg
     7321 ccatcatccg cggggccctg ggcatcacct gctcgatcct gatcgtggcg atgatcgccg
     7381 acgtggtcga ggacagccag gtgcagaccg gccgccgctc ggaggggctg ttcttcgccg
     7441 ccgtcagctt catcagcaag tcggtctcgg gcgtgggggc gatcatcacc ggcctgatcc
     7501 tgaccctgat ccacttcccg gtcggccagc cggccagcgc ggtgccgcag gcgacgatca
     7561 acgccctggg cctggcctat atcccgacgc tgttcgtgct ctacggcgcg gccctgttct
     7621 gcctgagctt ctaccgcatc acgcgcgaga gccacgcggc gaacctggag aagatcgccg
     7681 ccatggccag ggccgaggag gaggctatgc cgccgcctct gtgagggcgg cgtacaccag
     7741 ggtgcgcagc tcgcgccgga ccggataggc cgacgacggc agcagctggg tcaggaacac
     7801 cacggccagg tcctcgaccg ggtcgaccca gaagaaggtg ctggccgcgc cgccccagaa
     7861 gaagtcgccc ttgctgccgg ccagcatggt cgcggccggg tcgatggtca cgccgaagcc
     7921 caggccgaag ccgacgccgg cgtaggtcgc ctcgctgaac agcgatttcg acatctgcgt
     7981 caggtccttg ccgccgggca ggtggttggc ggtcatcagc tgcagcgtct tggggcccag
     8041 cagccgctgg ccgtccagct ccccgccgtt gagcagcatg cgggcgaagc gcatgtagtc
     8101 gtcggccgtg ctgaccaggc cgccgccgcc ggagtggaag ccggtctcgg tcaggtaggg
     8161 gctcttcacc ggatcgtcct gcagcttggg cgggccggcc gccacggcct tggagcccag
     8221 caccccggcg gcgtagcagg cggtcagccg gtgggccttg tcggccggca cggtgaagct
     8281 ggtgtcggtc atccccagcg gcccgaagat ccgctcctgc aggaaccgct cgaacgggat
     8341 cccgctgatc ctgcccacga ggtagcccag cacgtcggtg ctgaccgagt agttccaggc
     8401 gtcgccgggc gagaactgca gcgggatggt cgccagcttg gcgatgaact cgtcgagcgt
     8461 gccgaaccgc aggtcctcgg ccaccttcag cttgcggtag gcggcgtcga tgttgccctg
     8521 catctggaag ccgtaggtca ggccgctggt gtgacgcagc aggtcgatga ccagcatcgg
     8581 ccgcgcgggc ggcgtggtcg cccagcccgc gtcggtgccc gccgcgaaca cgcccaggtt
     8641 cgcccattcg gggatgtatt tggccaccgg ctcgtcgagc gcgaccagcc cctcctcgac
     8701 cagcatcatg aaggccaccg acgtgaccgg cttggtcatc gagtagatgc ggaacaggga
     8761 atcggccgcc agcggcgtgc cccgctcgcg gtcggccagg cccagcaccg tgtcgtaggc
     8821 cagcttgccg cgccgccaga cctgcacctg ggcgcagggc agcttgcccg actggatgta
     8881 gcgcgcctcg atgaacggcg cgatccgcgc caggcgttca gacgacaggc ccaggctctc
     8941 gggtttggtc acgatcacat ctccttgcga ggtctctcca attatcgaac cgccggtatg
     9001 ccgcaaccca aactttacca aacccgacgc agagtgacct tacgctaggg gacggacatg
     9061 gccgcaacga caggggcgaa cgccccctcc gagctcgaca tcgaggccgc gcgctggttc
     9121 ttccagaact cggtcgatgt cttcgtgctg atccgcggcg gcgagatctc gcggatcaat
     9181 cccgcctgga ccgagctgtc cggctggtcg gtcgaggaga ccgtgggccg gccgatcgag
     9241 gacttcatcc atcccggcga cgcgcagatc gtgctcgacc tggtcagcac gctgatcgcc
     9301 gatggccagg gccgggccga gcaccgcctg ctgcgcaagg acggcgagat cctgtgggtc
     9361 aagtcgcggg tcaagcgggc cgccggcgac gccacgatcg tcgtgctcga ggacgtcacc
     9421 gacgaacacc gtcgccgggc cgacagcgcc gacgcggcgc gcaccaacga cctgcttcgc
     9481 gaggccgccg gggtctacat ctggcggttc gatccccaga ccgggctcta cgacatcgac
     9541 accgacctgt cgaaggccgg cgcgcccggc gcatcgggca gccggacgat caccgccgac
     9601 gagatgacgt cggagatcca ccccgacgat caggcccggg tgggcgaggc cttcttccac
     9661 accctcgcca ccggcgagcc gcgggtggtc gagtatcgcc acttccgcgc cgaggacggc
     9721 ggctgggccc ggctgcgggc ggcctggcgc ggccttcgcc ggcgggagtc cggcaagtgg
     9781 gacgtgctgg gcctgaccca ggacgtcagc gaggtcgccg aggcgcgcga tgcggccctc
     9841 aacgccgccg aggccaaggc ccagttcctg gccaacatga gccacgagat ccgcacgccg
     9901 atgaatggcg tgctgggcgt cctgcacctg ctcaagaacg agaacctgtc gcccgacggt
     9961 cgccagctgc tggaggaggc cgtcggctgc ggccagatgc tggccgagct gctcaacgac
    10021 gtgatcgact tctccaagat cgaggccggc cggctggaat tctcgcccga gccgatcgat
    10081 ccggcggtca tgctcgacag cgtggccggc ctgctgcggc cccaggccgt ggcccgcggc
    10141 ctgtggctca acacccatgt ccatccggac ctcggctggg ccatggtcga tccggtgcgg
    10201 ctgcggcaga tcctgttcaa cctgatcggc aacgccgtga agttcaccct caacggcggg
    10261 gtcgatgcgc gcctgagccg ggtcgagcgc gacggcgtca gccggctgcg gttcgagatc
    10321 tccgacaccg gcgtgggcat cgggcccgac gcccgggcgg acctgttcca gcgtttccac
    10381 caggccgacg gctcgaccac ccgccggttc ggcgggtcgg ggctgggcct ggccatcagc
    10441 cgccggctgg ccgagctgat gggcggcgag atcggttacg acagccagct gggcgagggc
    10501 tcgaccttct ggttcgagat cgcggcgccg ccggtcgagg cccgcgccac ggaatccggc
    10561 gcgggcgacg gcctgctcga ggggcttcgc gtgctggtcg tcgaggacaa tccgaccaac
    10621 cggctgatcg ccacccgcat gctgcagcag ctgggcgcgc aggtggagac cgccgacgac
    10681 ggcggccagg gggttgaggc ggtccaccga tcggccttcg acctgatctt catggacatc
    10741 cagatgccgg tgatggacgg gctggaagcg acccgccgga tccgcgccat ggccgagccg
    10801 gtgcgatcca cgccgatcct ggcgatgacc gccaacgcga tgtcccacca ggcggaatcc
    10861 tacctcgccg cgggcatgaa cggcatcgtc gccaaaccgc tgtcgcccgc ggccctggtg
    10921 gcgcagatcg cggctgtcct agcgacggcc gcccgggtcg cggcggcctg agggcggacg
    10981 ctacccgttt gcgcaccgcg ctctgaacac cctttccctc gccatacccg atattctagg
    11041 ctggccggcc actggaacgc accgaggaga ccgcatggcc tggcccgccc gcagcatcgc
    11101 cgactgccac gcgatcctga ccgctcccgg ctcgcgcttc gagacggaag acgtggtcat
    11161 cgacggcgtc ccgacgaagg tctggaagaa cggcccggcg accctgcggg acctggtgac
    11221 caacggccgc gccttcgccc ggcgcgactt cctcgtctac gagggtgacc gcgccacgtt
    11281 cgagggcttc caccgggcgg tggcggccct ggccgaacgc ctggcggcag acggcgtacg
    11341 caagggcgat cgcgtggccc tggtcatgcg taacctgcct gaatggccgg tggctttctt
    11401 cgcggccgcc agcctgggcg ccatcgtcac cccgctgaac gcctggtgga ccggggcgga
    11461 gctgcagttc ggactggccg attccggcgc ccgcatcgcc atcgtcgacc ccgagcggct
    11521 ggaccggatc ctcgaccatc tgccggattg tccggcgatc gagcggctgt acgtctgccg
    11581 gcacgcgggc gcgatcgacg atgcgcgcgt tcaccggctc gagtcggtca ttggccccgt
    11641 cggagcctgg gagaccctgc cggaccggcc cctgcccgtc acgccgatcg cgcccgacga
    11701 tccggcgacc atcttctata cctccgggac caccggccgg cccaagggcg ccctgggcac
    11761 gcacagaacc atgacgacca acgtcatgac caccggcgcc gccctggcgc gggccttcct
    11821 gcgacgtggc gaaacgccgc cggttccgtc gccggacgcc ccgcagcggg tgacgctgct
    11881 ggtggttccc atgttccacg tcaccggctg ttcggcgacc ctctgcggcg cgctcaacaa
    11941 cggcggccgg ctggtgctga tgcgcaagtg ggacgcgctc gagggcatgc agctgatcga
    12001 gcgcgagaag gtcaccgcca ccggcggcgt gccgaccatc gcctggcagc tgatcgagca
    12061 tccacggcgg tccgagttcg acctgtcctc gatcgagagc atctcctacg gcggagcccc
    12121 ctcggcgccg gagctggtgc gccggatcaa ggaggtctgg ccatcggcct ccgccggcaa
    12181 cggctggggc atgaccgaga ccagcgccac cttcaccagc aacaacgccg aggattacga
    12241 gcgccgtccg gacagctgtg gcgtcgcccc ggccgtcggc gagatgaaga tcatgtcgcc
    12301 ggacggcgcg cgggaactgc cggcgggcga ggtcggcgag ctgtggtgca agggccccat
    12361 ggtcctgcgc agctactgga accggccgga cgccacggcg gagaccttcg tcgacggctg
    12421 ggtccgcacg ggcgacctgg cgcggctcga cgaggagggc ttctgcttca tcatcgaccg
    12481 ggccaaggac atgctgatcc gcggcggcga gaacatctac tgcatcgagg tcgagaacgt
    12541 cctctatgac caccccgcgg tgatggacgc cgccctggtc gggataccgc accgcacgct
    12601 cggcgaggag ccgggcgcgg tcgtgaccct caagcccggc gcggacgcct cggagcagga
    12661 gctgcgcgcc ttcgtcctcg agcggctggc cgccttcaag gcgccggtcc gcgtggcctt
    12721 ctggcccgaa accttgccgc gcaatgcgaa cggcaagatc atgaagtcgg aactcagatc
    12781 catcttcatc caggacgtcg tcgcttgacc gtcgaagccg tattgccccg accgtccgcc
    12841 acggcgcggt acgccgccaa gaaggaagcg atcctcggcg ccgccacaac catcctgaac
    12901 cggcaggggg tcaagggcat gaccctggcc gacgtcgccg ccgaagtggg cctgatcacc
    12961 acctcggtga cgtactacta tcgcaggaag gaggacctcg cggccgcctg cttcatgcgc
    13021 ggcatcgaca tgttcaacgg cctggcggcc gaggcggcga ccgtcgccgg cgcccgcgcg
    13081 cggctggcgc ggttcctcga actgttcttt gacctgtcgc accgcatccg cacccgggtg
    13141 accgcgccga tcgtggcctt cggcgagatg cgcgccctca aggagccgct gcgcacccag
    13201 gtttccgacg cattcaatga cctgttccgc accatccggg gcttcttcga cgatcccgag
    13261 ttcgccggac tggaccgtcg cgaggcgacg gcccgcaccc atctgctgct tgagcaggcc
    13321 ttctggaccg tgacctggct gcgccggtac gacatcgagg attacccccg cctgcgcgac
    13381 cggatgttcg acatcctctc ggggggcctc gccgtcggcg cgcgcgcctg ggccccgccg
    13441 tccctgccgg aggccgcccc gctggagtcc ggccgccacg atatctccca cgagaccttt
    13501 ctcgtggccg ccacgcggct gatcaatcgc aagggctatc gcggggcgtc ggtggaggag
    13561 atctcggccg agctgaacgt gaccaagggg agtttctatc atcacaacga agccaaggac
    13621 gacctcgtcg ccgaatgctt cgagcgcacc ttcgtcgtga tgcgggatat ccagttcgcc
    13681 gcccgggacc tcaaggtgga ccagtggacg cgcctggcgg ccgccagcgc cgccctcgtg
    13741 gaataccagc tgtccgatcg cggccccctg ctgcgcgcct cggccatgtc ggccctgccg
    13801 accgacctgc ggcacgagac ggtggagcgc tacaatcgcg tgacggaccg gttcgcggcg
    13861 atgatttcgg acggtatcgc cgagggctcg attcgcccgg ttgatccgct ggtggccgcg
    13921 cacatgctca gctcgatgct caacgccgcg gcctcccttg ggcaatgggc gccggggctc
    13981 aagccggagg aggccgcggc gctgttcgcc cgccccctgc tgctgggcgt gttcacccgc
    14041 tagagcgcgt tccggttggt cgggccggta atcggacaaa acgcgcgacg atgaaaagtc
    14101 tcgagcggcc gatttgattc catcaagtcg gatcccgctc caggcccgcg tccggacgca
    14161 gccgggcaag aaaaaggggc ggccgaagcc gcccctgaag tgatcgtccg ggtctgtcgc
    14221 ctagaacttc ttcacgacgt tgaacttgat ggtccgtccc agcgcgctgg ccgtgaacgg
    14281 gtcgtagttc aggtcgagac gcgccagggg cgggtcctga tcggtgatgt tctccaccgc
    14341 cgccgagaag gtcgtgtccc agggcaggaa cacgcggtag gctaggtcgc cggtggcata
    14401 ggcgtcgatt tccttgcccg cgccggtgat ggcggcccgc tggtcgcgat aggagtccac
    14461 gtaccgcacg gtgaggcgca ggttgtggtc gccgttggtg tactcgaagt gcacgtcgcc
    14521 cttcatctgg ggcagcgggt aggcggtcgt ctggtagttc agctgaccca ccgcgtcgaa
    14581 ggccggcgcc acggtgacgc cgccgacggc gagcgcgtcg gtcttgtact cgaaggtgta
    14641 ggtcaggctg gtgccgaccg tgaagctgcc gccgaggaac tcgtcgaacc gccagtcggc
    14701 cagcaggtcg aggcccgagg tcttcaccgc cgcgccgttg accgtgaggg tcctcagacg
    14761 ggagatgttg gtcgtgctgc aggcgccgcc gaaggtgaag cgggcctcga ggccggcgta
    14821 ggccggatcg ccgcagtgac cggtcgtacc gagcgccgcc gggaacatcg tggcgacgat
    14881 gtcgcccgag ggttcggcca cgatcgggtt gtcgaagtca aagcgccagt agtcgagcga
    14941 ggccttgaac ccgccagcct ttacgatcag gcccgcgctg taggtgttgg ccgtttcagg
    15001 ctcgagattc gggttgccgt agatgtcgat ggcccggaac gacccggcga tgaactgcag
    15061 ggaggtcacg aagccgccgg ccagattggt ctgcggcgga ccccggaagg tggtgccggc
    15121 cgagccgcgc agggccagcc agtcgttcac ctgccagcgg gcggcgatct tcgggttgaa
    15181 ggtggagccc acctcgccac cgtagtcctc gtagcgcgcc gccagctgga cgttgaagtc
    15241 gtcggtgaac ggcagctgca gttcgccgaa cgtcgcgtag acgtcgccct ggaggtcgga
    15301 ctcgaacccg gcgcccagga agaagaaggc cccgttgcgc accgtgcagg cggtcgagcc
    15361 gttgaacggg gtgtcgatgc acggattgac gttgccgtcg ctgatattgt tgtagtcgga
    15421 gatgaagtac tcgcggcgat actgcacgcc gagcgcccag gccggctgac cgccgcccag
    15481 gtcccagttg agcttgccgt tgatcaggcc gtcggcaacg aacagacgct gggtctgctt
    15541 ggtgctcagc ttgcggaaga accagcggac cagatcgaca ttgttggcca cggccgaatt
    15601 gtagccaggg ttggtcgcgc cggtgatcga gttgctctgc accgcgttgg agaacgggtt
    15661 gaagtagtag cagcccaggg cgttgttgcc ggcgttggtg gtgtagttgg cggtttccgc
    15721 ggcggtgcag ccgctggcgt cgccggccag actgccgtag ccgcgcaggg ccatctggaa
    15781 gaacggcacc acggtgtcgt agcccgtccg ctcgccggtt tcctgcgagt aggtcagcgc
    15841 cacgttccag ccgatgccgt tgccgaactc acccgtcagc tcgccgttga tgcgaaccgc
    15901 ctcgaattcc cgcgaacctt ccgacggtcc gtcgccgaac atcgggttgc cgcccatggc
    15961 gaacggccgg tagagcacgc cggggaagaa ggccccgttg gcgaaggccg gcagctgggc
    16021 cgggttggcc gcctggtagg caataaaacc ggggttgctg ctgggcaccc agtagccggc
    16081 caggttggct acccgtggcg cggggctggt cgcagtggtg ggcgactgca gcgccaggta
    16141 ggacggcgag gtcttccact ccggcacgtc ggtgtgggcg tagagcagct cgccgtggaa
    16201 cgtcgtggtg tcggtgacgt cgatgttcac ctcaccgtag agctggtagc gatcctcgat
    16261 ctcggtgatg ttgtcgtagg gcgtgtagtg gaagccgcag ccgcccgccg tcgcccgccc
    16321 gcccaggggc gcgcaaccga cgtcggtctg caggcccgcc gccggcgcat agatgcccgg
    16381 cgcggggctg aaggtcggca ggaaggccat gccgccgccg cccgtccagc cgccctgcgg
    16441 gttgctggcg aagtcgaggt tggcccagtc gcgctcggtg accggaagct cggaccggtg
    16501 ctggaagccg gccgtcagca ggatgttggc gctgtcgccg acccagcccc agacggcgct
    16561 gccggtatag tcgccgtcag acccttcgac gctcttgtag ctgcccgcga cctcgaggcc
    16621 ctcgaagttc ttgcgggtga tgaagttgac caccccggcg atggcgtcgg agccgtaggt
    16681 cgccgccgcg ccgtccttca gcacctcgac ccggccgatg gcggccgagg ggatgatgtt
    16741 ggtgtcgacg atgccggtgc cgacctggcc gaacgggttg ggcgtgatgc gccggccgtt
    16801 catcagcacc agggtgcgct gcgagccgag gccgcgcagg ttgaccgaac ccgagccctc
    16861 cgaaccctgc gcgcgggcgt cgaactggtt ggtctcgccg agaatgccgc tgctcaccgg
    16921 cagggcgcgc aacagctcta cggtcgaggg cgagccctgt ttcgacagtt cttcggcgcc
    16981 gatcacgtcg accggcatgg ccgcgtcttc cggcgtgccg gcgatcagtg agccggtgat
    17041 gacgacagct tcgacttccg tcggctgggt cgcctgcgcc gaggcggtcg aagccgctcc
    17101 caacgacagg gcgatggcga gggcggagcc cccggccaga tagccaattt tccgcattgt
    17161 accctccctg ttatctccca tgccgtcccg tctttgtttg aattcgaatg acggttacgg
    17221 cgttaccgcc agcgctacgg acctcccgag aaaggcgtag acacatgcgc atgcaccccc
    17281 ggccgcggcg cggccggacg atccgagtga cgaggattgg agggtaaaac ggtccggcgg
    17341 gcgtcatgcg cccggtctga agacccgatc atggtgcgcg aagaccgcgt caaccagccc
    17401 cctctaggct catcccaccg accggatcac gggctcgcct cgcaggccgc gaccgccgca
    17461 atcagatcgg caaacaggtt tcgatgatgc ggtatgattt gcccatgcgt caaccggact
    17521 cgcataacag cgtccaatat tcgccggcct tctctgtggg cgcagagccc cccattcatc
    17581 cggtgtccgg accggcgccg atggcctgtc gcggcggggt cgatcctcca gcaggacggt
    17641 agagcgggcc gatccttgcg gcgggcgtat ggggtaaatg ccggatttcc cgacgattcc
    17701 cgcgcccgcc ggaggtctgc ggcgatcggg gtgcgggacc cggccctggc catcgcgcgc
    17761 cgcggcgccc cgcct
//
LOCUS       DAORNX010000008        17016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2551_length_17016_cov_7.936266, whole genome shotgun sequence.
ACCESSION   DAORNX010000008 DAORNX010000000
VERSION     DAORNX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 17016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17016
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_17016_cov_7.936266"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLO65_00720"
     CDS             complement(<1..802)
                     /locus_tag="PLO65_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRD26802.1"
                     /translation="MFDALTERLGSVFDRLSGRGVLGEKDIDEALREVRVALLEADVA
                     LPVVKTFISKAREDALGEAVIRSIRPADQVVKIVYDGLVEMLGGETPTGLQLALNPPS
                     IILMAGLQGSGKTTMSAKLGLRLSKVERKKVLMASLDTRRPAAMEQLAILGTQIGVDT
                     LPIVAGQSAVDIARRALQVARLSGYDVVILDTAGRTTLDEQMMSEAAQIAQIATPTET
                     LLVADSLTGQDAVRTARAFHERLPLTGLILTRADGDGRGGAALSMRAVT"
     gene            complement(1156..1575)
                     /locus_tag="PLO65_00725"
     CDS             complement(1156..1575)
                     /locus_tag="PLO65_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD26803.1"
                     /translation="MVRAYLFWMIEITGIDHLVLRAKDPDALIAFYTGVLGLTVEKVQ
                     AGIGLTQLRAGTSLIDIVAVDGKLGRMGGAAPGAEGRNLDHVCLTVRGFDLETVKAHL
                     IAHGVTPGEEGLRYGAGGEGLSIYLTDPEGNGLELRG"
     gene            1667..2254
                     /locus_tag="PLO65_00730"
     CDS             1667..2254
                     /locus_tag="PLO65_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26804.1"
                     /translation="MGRILLAGLLGGVAMFIWTSLAHTVLPLGQMGIQEIPDDAPVIA
                     TLQGALGDRDGLYIFPGSGNGAKSENAASRETMEARTAKEASGLLVYHPPGRDTAMGP
                     YIGKELTLEIFQALILAWLIATSTASGLGGRTLAAAAVGLVVAFATNGSNRIWYGFPT
                     DFTYAAILIQVVGYTVAGAVIAVILGMKRRTAASG"
     gene            complement(2355..4430)
                     /locus_tag="PLO65_00735"
     CDS             complement(2355..4430)
                     /locus_tag="PLO65_00735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRD26805.1"
                     /translation="MTTVSGRALAAASGNPLLPPFAGPHGGLPPFDRIKTADFAPALD
                     EAIAINRKEIAAIVANPAAPTFENTLAAMENSGRELTRTAVIMGVYTSTMSDKPMQAV
                     EQALSPKLAGYGDEIVQNEGLFKRVKAVYDARESSGLTSEQQRLAWRYYQNFARQGAA
                     LDGDKKKRLGEINQRLATLYTTFSQNELADEESYTLELGPDDLAGLPESIKANAAGAA
                     EEKGLKGKWLITNTRSSMEPFITYSSNRALREKGWRMWIMRGDNGDAHDNNAIITEIL
                     KLRAERAGLLGYATHAHWITADQMAGTPDKAMDLMLRVWKPAVARVHEEVAMMQKIAD
                     AEGAGITIEPWDYRYYLEKVRLAEYDLDQNEVKPYLQLEKMREAMHWAAGQLYGFTFT
                     QISDVPVVHPDVRVWEVTRDGQRVGLWYFDPYARAGKQSGAWMNEYRTQENFVGPVTA
                     IVSNNCNFIKGKPGEPVLISWDDASTLFHEFGHALHGLNSAVHYPTLAGTNVARDFVE
                     FPSQLNEHWLPTKEVLNQFALHYQTGKPIPAELVAKIERADKFGQGFATVEYLASALV
                     DMKAHLAGATPIEPRAFEKATLAEIGMPKEMVMRHRMPQFGHIFAGDGYSAGYYDYIW
                     ADTLTADAAEAFAEAPGGFYDKAVAKRLHDSIMSVGNTIDAGEAFRRFRGRDVDTGAL
                     MRDRGFPVA"
     gene            4597..5448
                     /locus_tag="PLO65_00740"
     CDS             4597..5448
                     /locus_tag="PLO65_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRD26806.1"
                     /translation="MSIGVFDSGVGGLTVHRALVERFPQADFVYLADQANAPYGGRPG
                     EEIVDLTRAGCIRLFDDGASLVVLACNTASAIALRRLQQTWLPGYRRETGRALNVLGI
                     IVPTIEAATGLPWEHEADRRGEQVTKVDVLGVFSTPGTAGSRVFEIEIDKRKQDLAVF
                     SEPCPNLARMIEDNADRAALASEIAGHVDALRRRIGRHPDRAILGCTHYEIVADLFRD
                     ALPPGTPLIHQPDATAEALGRYMERHPEYVAGGTGLRRFLTTGTPGPQNGLVATFWGE
                     PLAFEAA"
     gene            5511..6050
                     /locus_tag="PLO65_00745"
     CDS             5511..6050
                     /locus_tag="PLO65_00745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD26807.1"
                     /translation="MKILKTVAAAALLGLATPAHAEVKQTWAAGFRLENRAVIAASPD
                     RVWAALGQIGKWWNGEHSYSGDSANMTMPLTPGGCFCEALPKLGAGAGVQHGVVVMIM
                     PGQTLRLDAPLGPLQGEGVSAALTFTLKATADGGTEVVETFNVGGARPEIVGYAAGID
                     GVVGEQLGRLKAYVETGKP"
     assembly_gap    6094..6112
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6138..6674)
                     /locus_tag="PLO65_00750"
     CDS             complement(6138..6674)
                     /locus_tag="PLO65_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD26808.1"
                     /translation="MSLDALREALPAYAKDISLNLSSLAGETLLTDQQKWGAFVASAH
                     AVGVPVVVRSLEAAAREAGLSDEALSAAKGAAAIMGMNNVYYRALHLVKNKEYATLPA
                     RLRMNLLANPGVDKVDFELWSTAVSAVNGCGMCLDAHEAELKKHGVPNTTIQAALRIA
                     AVVNAASRVVAGEAAAAA"
     gene            complement(6907..7467)
                     /locus_tag="PLO65_00755"
     CDS             complement(6907..7467)
                     /locus_tag="PLO65_00755"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD26809.1"
                     /translation="MLGVGQKLPEFRIVGVKPGFMRHEENGESAFETLTNASFDGKWK
                     VIFFYPKDFTFVCPTEIAEFARLNKEFADRDTVVLGGSTDNEHSKLGWRREHPDLHKL
                     PIWSFADNSGKFARALGVLDEDEGVAQRATFVVDPHGVVQHVYVTNLNVGRAPADTLR
                     VLDALQTDELCPCNRAVGGDTLDLAA"
     gene            7615..8508
                     /locus_tag="PLO65_00760"
     CDS             7615..8508
                     /locus_tag="PLO65_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRD26810.1"
                     /translation="MLPTIRQLQYLKLLSDHGSFSRAAEAAHVTQPTLSAGIAELEKI
                     LGAPVVDRARSGVILTAAGEEAVRRARVILEQTDDLVQAAQGAGQPLTGRFRLGVIPT
                     VAPFLLPRALPVLRKQFPKLRLFLREDLTHRLIASLKAGALDAALIALPYDMTGLAWA
                     HVEDDELLAAAPNGHPILDQKRVNPEDLAPKDLILLEDGHCLRDHALAACGLEPPKST
                     DEETFAATSLPTLVQMVGSGLGVSFLPAMAVNAGLTEHVGVTTRPLQAEHPSREIVVA
                     WRAGSSRANEGRMLAEVLRGV"
     gene            8685..9242
                     /locus_tag="PLO65_00765"
     CDS             8685..9242
                     /locus_tag="PLO65_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRD26811.1"
                     /translation="MHRHFGFSHHKHERFGRHGFGGHHGRHMGRGGRFGRFFDHGDLK
                     LVILRMIADQPRHGYELIKEVEEQAGGAYSPSPGVVYPTLTWLEEMGFVTAADAAGGR
                     KLYTITAEGEAHLRANEGQTASIFQRMAEAAAASTAFSPQILRARENLRTALKLKTTG
                     GALSKDQIESICDALDAAAAAVERA"
     gene            complement(9330..11414)
                     /locus_tag="PLO65_00770"
     CDS             complement(9330..11414)
                     /locus_tag="PLO65_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD26812.1"
                     /translation="MTAAAFTASAQPAPTPADPFTWMEEIEGTRALDWARAENARSLP
                     VLQNDPRYEGLHQSALDIVNARDRIPGVSFSGDGTLGNFWQDAEHVRGLWRRTSVDSF
                     RTADPVWETVLDVDALARAEGQNWVYKGATCLAPDDRYCLVQLSDGGKDAVTLREFDT
                     VTKAFVEGGFVSPDGKQNASWLDKDTVLIARDWGPGTMTKSSYPFVLKLWRRGTPLEQ
                     ATEIYRGTADDVSVSPFVLRDADGKVQAVIAARSPSFFETEYWLITDQGPIKLPFPMK
                     TALQGLVGGQLVFTLQEDWPSRSLKQGDLASIDLQSLIGEPDRASAVLILRPTPSQAV
                     EQINITRNRLIVGLTENVTGQAWVYTPGGDGAGPTTWTGRKLDLPANITIGVGSASER
                     DDRVFFNVSGYLTPSTLWLADAASGKVEKIKELPPRFDASTHVVEQFWATSKDGTKVP
                     YFVVRPKDLKYDGTAPTLQYAYGGFQVSMTPGYSATVGKLWLERGGVYVVANIRGGGE
                     FGPAWHEAALKANRQRAYDDFFAVSEDLIARKITSPRRLGIMGGSNGGLLMGVALTQR
                     PELYNAVVIQVPLFDMLAYTHIGAGASWVGEYGDPAIPEERAWIAAYDPYSNLKAGKP
                     YPQVFIETSTKDDRVHPAHARKAAARLEALGYSFLYYENIDGGHSAAANLNETAKRVA
                     LEFTYLSQRLMD"
     gene            complement(11444..13573)
                     /locus_tag="PLO65_00775"
     CDS             complement(11444..13573)
                     /locus_tag="PLO65_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD26813.1"
                     /translation="MKFSAFLAAAAVLVAAPVMAATETPFEWLEQVDGDRSMDWVRAE
                     NAKTLPVLTGDARFAGLQAETLKILSASDRIATPTFVGDQVFNFWQDATHVRGVWRRT
                     SLASYRTADPQWETVLDLDALATAEHANWIWKGADCRPATHDRCLVELSNGGKDAVEI
                     REFDLKTKAFVDGGFRLPESKQTVAWLDADTLILTRDWGPGTTTESGYGFVVKTLKRG
                     QTLDQAVEVFRGDVKDVSATARVLRDGDGRRVVLLEQGLDFFHTRYAMLDADGVTPLA
                     IPQRSRIYGLVGGDVIFSIEEDWTRPRWVGDDKPPTFAAGSLMSATPHALKPQPAGVL
                     MGNDCDPCLLLASAPRRSIDQVAVTDHRVVAVVYENVRASVMTFVDRGEWGWKSATLP
                     VIDNGSAALVATADKGDQLFYSVEGFTTPTELKLADAATGQSETVKALPARFDASGLM
                     VEQHEATSKDGTKIPYFIVTRKGHSHDGSNPTLLHAYGGFQLSKLPVYDPIVGKLWLE
                     RGGTYVVANIRGGGEFGPAWHEAALKANRQRAYDDYFAVAEDLIARKITSPRRLGAYG
                     RSNGGLLMGVAMTERPDLWNAVVVESPLLDMLRYHLLPAGASWIGEYGDPTIPEEAAW
                     IAKYSPFQNLRPGVKYPLAYITTSTKDDRVHPGHARKFAAMLAQLGAPYLYYENTDGG
                     HANGADPTANARRWGMHYTYLTRQLMD"
     gene            13732..>14036
                     /locus_tag="PLO65_00780"
     CDS             13732..>14036
                     /locus_tag="PLO65_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRD26814.1"
                     /translation="MTRPFLKMNGLGNDFVVVEARGEPFRPTPAEARAIADRESGVGC
                     DQLIGIEPTRRADAFMRIWNADGGEVDACGNATRCVAWLLMEATGRDEAVIETEAGLL
                     "
     assembly_gap    14037..14124
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14125..14547
                     /gene="dapF"
                     /locus_tag="PLO65_00785"
                     /pseudo
     CDS             14125..14547
                     /gene="dapF"
                     /locus_tag="PLO65_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921513.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
     gene            complement(14915..15289)
                     /locus_tag="PLO65_00790"
     CDS             complement(14915..15289)
                     /locus_tag="PLO65_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD26815.1"
                     /translation="MASVSYITVGSNDLEAAKGFYDALLGSIGMKGVFEHPTGGRLYR
                     GKATGLFGVLGPYDGGAASVGNGMMGGFSFDSREEVDAFHAKALELGGTCEGPPGERQ
                     PGAYFAYFRDLDGNKLCGFKFG"
     gene            15485..15988
                     /locus_tag="PLO65_00795"
     CDS             15485..15988
                     /locus_tag="PLO65_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26816.1"
                     /translation="MSRFTVLAAIGALAVATSVAVPMPARAESAAVSATCNTLYTTST
                     AVLGTAATVGPASVGYPGQASAQGCQIAFSGDGTQYGTNFQAVADKLDAMMLGAGWTR
                     DHNADADGPTGTATGYKKGGELVAVSVGYDTPAGVCRKDEPSASCHPTAAQMLYTITL
                     GVVAAGS"
     gene            complement(15996..16529)
                     /gene="thpR"
                     /locus_tag="PLO65_00800"
     CDS             complement(15996..16529)
                     /gene="thpR"
                     /locus_tag="PLO65_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRD26817.1"
                     /translation="MIRLFAAIAIPEEIGEPLLRRQQGLPGARWRPLEAFHITLRFFG
                     DVQERVAEDLDLELSHITSAPFAVTLDGVGAFGEGPDIHAVWAGVAENPPLRTLAGRC
                     ETAARRARVTPDGRTWRPHLTLAYLRRPDPTDVAVWIQANNLLRSPPFAIDRFGLYSS
                     HQTGEGSRYVLEREYSL"
     gene            complement(16526..>17016)
                     /locus_tag="PLO65_00805"
     CDS             complement(16526..>17016)
                     /locus_tag="PLO65_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRD26818.1"
                     /translation="LPAQLEVALTRLVPGVRVRSAGVSGDTSAGGAARVDFSVQKDTA
                     VCVVALGGNDLLQGLDPKQTRANLDRIIRRLQARGITVVLAGLSAPTAIGRNYAREFG
                     AVFPTLARQRGVPLYPNLLAGVAGDPRLNQRDGIHPNAAGVRIIAAGLAPVVARALAK
                     AR"
BASE COUNT         2543 a         5722 c         5922 g         2722 t
ORIGIN      
        1 cggtgacagc ccgcatcgac agggccgcgc cgccgcgccc gtcgccgtcg gcgcgggtca
       61 ggatcaggcc ggtcagcggc aggcgttcgt ggaaggccct ggcggtgcgc accgcgtcct
      121 ggccggtcag gctgtcggcg accagcaggg tctcggtcgg cgtggcgatc tgcgcgatct
      181 gcgccgcctc gctcatcatc tgttcgtcca gcgtcgtccg gccggcggtg tcgaggataa
      241 ccacgtcgta gccgctgagc ctggccacct gcagcgcgcg cctggcgata tcgacggccg
      301 actggccggc gacgatcggc agggtgtcga ccccgatctg ggtccccagg atggccagct
      361 gctccatggc ggcggggcgg cgggtgtcga gcgaggccat caggaccttc ttgcgctcga
      421 ccttgctgag ccgaaggccc agcttggcgg acatggtcgt cttgccggag ccctgcaggc
      481 cggccatcag gatgatcgac ggcgggttca gcgccagctg cagtccggtc ggcgtctcgc
      541 cgccgagcat ctcgaccaga ccgtcataga cgatcttgac cacctggtcg gccgggcgga
      601 tcgagcggat gaccgcctcg cccagcgcgt cctcgcgggc cttgctgatg aaggtcttga
      661 ccaccggcag ggcgacgtcg gcctcgagca gggcgacgcg cacctcgcgc agcgcctcgt
      721 cgatgtcctt ttcgccgagc acgccgcgac cggagagccg gtcgaagaca ctgccaagtc
      781 gttctgtaag ggcgtcgaac atgccgtcct gaaccgtcaa acccaagtcg aaggcccaaa
      841 cgcaattcgc ccccgtggac gatcacgtcg acggggggcc tcgccgtcct cgtcccgacc
      901 tgaagtctgc cggggggcgt gacggtggag ggcgctgaac tgcatctgcg ccggaaggcg
      961 ggccttatgg gcggaaaagg ccgggaaatc aagctacgga atcccgctcc ccttgcggga
     1021 gaaggtgtcg gcgtagccta cggatgaggg gtttggctga attcaaggtt ggtgcggagg
     1081 tgaggcggcg cctgcccacg acccctcatc cgacccgctt cgcaggccac cttctcccgc
     1141 aaggggagaa ggaaactagc cccgcagttc caggccgttg ccttcgggat cggtcaggta
     1201 gatcgacagg ccctccccgc cggcgccgta gcgcaggccc tcctcgccgg gcgtcacgcc
     1261 gtgggcgatc aggtgcgcct tgaccgtctc caggtcgaag ccgcgcacgg tcaggcagac
     1321 atggtcgagg ttgcggcctt ccgcgcccgg cgccgcgccg cccatccggc cgagcttgcc
     1381 gtcgacggcg acaatgtcga tcaggctggt tcccgcgcgc agctgggtca ggccgatgcc
     1441 ggcctggacc ttctcgacag tcaggccgag cacccctgtg tagaaggcga tcagcgcgtc
     1501 cgggtccttc gcgcgcagga ccaggtggtc gatgccggtg atctcgatca tccagaacag
     1561 ataggccctc accacgcgcg gttgaagggc ggtgatctcg acgccggccc gttacgggcc
     1621 tagcgtccgc caggcgccgt tatccatctc tgggggagat cagatcatgg gacgtattct
     1681 gctggccggc ctgctgggcg gcgtcgccat gttcatctgg acatcgctgg cccacaccgt
     1741 gctgccgctg ggccagatgg ggattcagga aatacccgac gacgcgccgg tcatcgcgac
     1801 cctgcagggc gcgctgggcg accgggacgg gctgtatatc ttcccgggct ccggcaacgg
     1861 cgccaagtcc gagaacgccg cctcgcgcga gacgatggaa gcgcgcaccg ccaaggaagc
     1921 ctcgggcctg ctggtctatc acccgcccgg ccgtgacacc gccatggggc cctacatcgg
     1981 caaggagctg acgctggaga tcttccaggc cctgattctc gcctggctga tcgcgacgag
     2041 cacggcgtcg ggccttggcg gtcgcacgct cgccgccgcg gccgtcggcc tcgtcgtggc
     2101 cttcgccacc aacgggtcga accggatctg gtacggcttt ccgacggact tcacctacgc
     2161 ggccatcctg atccaggtgg tcggctacac cgtcgccggc gcggtgatcg ccgtcatctt
     2221 aggcatgaag cgcaggacgg ccgcgtcggg ctgagtgcgt ggttcgacac gcaccctgcg
     2281 ggtgctgctc accatgacgt gcgcaaggca acctcatcct gagcaggccc gcaaggccgt
     2341 gtcgaaggac gcgatcaggc gaccgggaag ccccggtcgc gcatcagggc gccggtgtcg
     2401 acatcccggc cacggaagcg gcggaaggcc tcgcccgcgt cgatcgtgtt gccaacgctc
     2461 atgatgctgt cgtgcagccg cttggcgacg gccttgtcgt agaagccgcc cggcgcctcg
     2521 gcgaacgcct cggccgcgtc ggcggtcagg gtgtcggccc agatgtagtc gtagtagccg
     2581 gccgagtagc cgtcgccggc gaagatgtgc ccgaactgcg gcatgcggtg acgcatgacc
     2641 atttccttcg gcatgccgat ctccgccagg gtcgccttct cgaacgcgcg cggctcgatc
     2701 ggcgtggcgc cggccaggtg ggccttcatg tcgaccaggg cgctggccag atattccacc
     2761 gtggcgaagc cctggccgaa cttgtcggcc cgctcgatct tggccaccag ttcggccggg
     2821 atcggcttgc cggtctgata gtgcagcgcg aactggttca gcacctcctt ggtcggcagc
     2881 cagtgctcgt tgagctggct cgggaactcg acgaagtcgc gcgccacgtt ggtgccggcc
     2941 agggtcggat agtgaaccgc cgagttcaga ccgtgcaggg cgtggccgaa ctcgtggaac
     3001 agcgtcgagg cgtcgtccca gctgatcagc accggctcgc cgggcttgcc cttgatgaag
     3061 ttgcagttgt tggagacgat cgccgtcacg gggccgacga agttctcctg ggtgcggtac
     3121 tcgttcatcc aggcgcccga ctgtttgccg gcccgggcgt aggggtcgaa gtaccacagg
     3181 ccgacgcgct ggccgtcgcg ggtgacctcc cagacgcgga cgtcgggatg gaccaccggc
     3241 acgtcgctga tctgggtgaa ggtgaagccg tacagctggc cggcggccca gtgcatggcc
     3301 tcgcgcatct tctccagctg caggtacggt ttcacctcgt tctggtcgag gtcgtattcg
     3361 gccaggcgca ctttctcaag gtagtagcgg tagtcccacg gctcgatggt gatcccggcg
     3421 ccctcggcgt cggcgatctt ctgcatcatc gccacttcct catgcacgcg ggccacggcc
     3481 ggcttccaga cgcgcagcat caggtccatc gccttgtccg gggtgccggc catctggtcg
     3541 gccgtgatcc agtgggcgtg ggtcgcgtag ccgagcaggc cggcgcgctc ggcgcgcagc
     3601 ttgaggatct cggtgatgat ggcgttgttg tcgtgcgcgt cgccgttgtc gccgcgcatg
     3661 atccacatcc gccagccctt ctcgcgcagg gcgcgattgc tggaataggt gatgaacggc
     3721 tccatcgagg agcgggtgtt ggtgatcagc cacttgccct tgaggccctt ttcctcggcc
     3781 gcgccggcgg cgttggcctt gatcgactcg ggcaggccgg ccaggtcgtc cgggccgagc
     3841 tcgagggtgt agctttcctc gtcggccagc tcgttctggc tgaaggtggt gtagagcgtc
     3901 gccagccgct ggttgatctc gcccagccgc ttcttcttgt cgccgtcgag cgccgcgccc
     3961 tggcgcgcga agttctgata gtagcgccag gccagccgtt gctgctcgct ggtcaggccc
     4021 gaggattcgc gcgcgtcgta gacggccttg acccgcttga agaggccctc gttctggacg
     4081 atttcgtcgc cgtagccggc cagtttcgga gaaagggcct gttcgaccgc ctgcatcggc
     4141 ttgtcgctca tggtcgaggt gtagacgccc atgatcaccg cggtgcgggt cagttcccgg
     4201 ccgctgttct ccatcgccgc cagggtgttc tcgaaggtgg gcgccgcggg gttggcgacg
     4261 atcgccgcga tctccttgcg attgatggcg atggcctcgt ccagggccgg ggcgaagtcg
     4321 gccgtcttga tccggtcgaa cggcggcagg ccgccgtgcg ggccggcgaa cggcggcagc
     4381 agcgggttgc ccgaggcggc ggccagcgcg cggcctgaaa cggtggtcat ggccgcggcg
     4441 gccgcggtgg tggcgagaaa ggtacgacga tgcacgaaaa atctcccgag aatccggttt
     4501 gcggggaccc tatccccctt ttcgcccgaa cgtcacatga acggcctgta atgcgccgca
     4561 tggacgggtc gcgggcaggg cgctaaaggc gaatccatgt ccattggcgt tttcgattcc
     4621 ggtgtcggcg gcctcaccgt ccatcgcgcg ctcgtcgagc ggttcccgca ggccgacttc
     4681 gtctacctcg ccgaccaggc caacgccccc tacggcgggc ggcccggcga ggagatcgtc
     4741 gacctgaccc gcgccggctg catccggctg ttcgacgacg gcgcgagcct tgtcgtgctg
     4801 gcctgcaaca ccgcctcggc catcgccctg cgccgtctgc agcagacctg gctgccgggc
     4861 tatcgtcgcg agacgggccg ggcgctgaac gtgctgggga tcatcgtgcc gaccatcgag
     4921 gcggccaccg gcctgccctg ggagcacgag gccgaccgcc gcggcgagca ggtgaccaag
     4981 gtcgacgtcc tcggcgtctt ctccacgccc ggcacggccg gcagccgggt gttcgagatc
     5041 gagatcgaca agcgcaagca ggatctggcg gtgttctccg aaccttgccc gaacctcgcc
     5101 cggatgatcg aggacaacgc cgaccgcgcg gccctggcga gcgagatcgc cggccatgtc
     5161 gacgccctgc gccggcgcat cggccgtcat cccgaccggg cgatcctcgg ctgcacccac
     5221 tacgagatcg tcgccgacct gttccgcgac gccctgccgc cgggcacgcc gctgatccac
     5281 cagcccgacg ccacggccga ggccctgggg cgctacatgg agcggcatcc ggaatacgtc
     5341 gccggcggga ccggcctgcg ccgcttcctg accaccggaa cgcccggtcc gcagaacggc
     5401 ctggtggcga ccttctgggg cgagccgctg gcgttcgagg cggcgtgaac gatttgacac
     5461 ttgattgatt gatcaagcgg ccgtcatctc ccgcccaagg gagatcaatc atgaagatcc
     5521 tgaagaccgt cgccgccgcc gccctgctgg gcctggccac gcccgcgcac gccgaggtca
     5581 agcagacctg ggccgccgga tttcggcttg agaacagggc cgtcatcgcc gcctcgccgg
     5641 acagggtctg ggcggcgctg ggccagatcg gcaagtggtg gaacggcgaa cacagctaca
     5701 gcggcgacag cgccaacatg accatgccgt tgacccccgg cggctgtttc tgcgaggccc
     5761 tgcccaagct cggcgccggc gcgggcgtgc agcacggggt ggtggtcatg atcatgccgg
     5821 gccagaccct gcgcctcgac gcgcccctcg ggccgctgca gggcgagggc gtcagcgccg
     5881 ccctgacctt cacgctcaag gccacggccg acggcggcac ggaggtcgtc gagaccttca
     5941 acgtcggcgg ggcgagaccc gagatcgtcg gctacgccgc gggcatcgac ggcgtggtcg
     6001 gcgagcagct gggacggctg aaggcttatg tggagacggg caagccctga tccggcggag
     6061 ggggacatgt accgcaggta cgtgtccccg aaannnnnnn nnnnnnnnnn nncgaaggcg
     6121 gtacatgtcc ccttcgacta cgccgccgcc gcggcttcgc ccgccaccac gcggctggcc
     6181 gcgttgacca ccgcggcgat gcgcagcgcg gcctggatcg tggtgttggg cacgccgtgc
     6241 ttcttcagct cggcctcgtg ggcgtcgagg cacatgccgc agccattgac cgccgagacg
     6301 gccgtcgacc acagctcgaa gtcgaccttg tcgacgccgg gattggccag caggttcatg
     6361 cgcagccggg ccggcagggt cgcgtactcc ttgttcttca ccaggtgcag cgcgcggtaa
     6421 tagacgttgt tcatgcccat gatcgcggcg gcgcccttgg cggccgagag ggcctcgtcg
     6481 gacaggcccg cctcgcgggc ggcggcctcc aggctgcgca ccacgacggg cacgccgacg
     6541 gcatgggccg aggcgacgaa cgcgccccac ttctgctggt cggtcagcag ggtctcgccg
     6601 gcgagggacg acaggttcag gctgatatcc ttggcgtagg cgggcagcgc ctcacgcaga
     6661 gcgtcgaggg acatggcggt ctccgaattt cgcaagaggg gccgggagcc ccgcgtcggc
     6721 cggagggacc aggcgcgcgg ggctcccgac gacgcggccc tggggggcag ggggccgcgt
     6781 gtgggtgttg ggatcgtgcg tggttcgaca cgcaccctgc gggtgctgct caccatgacg
     6841 ttcacagatg cacgtcatcc tgagcaggcc cggagggccg tgtcgaagga cgcacccagg
     6901 cctgacctag gcggccaggt cgagggtgtc gccgccgaca gcgcggttgc aggggcacag
     6961 ttcatcggtc tgcagcgcgt cgagcacccg cagggtgtcg gccggggcgc ggcccacgtt
     7021 caggttggtg acgtagacgt gctgcacaac gccgtgcggg tcgaccacga aggtcgcgcg
     7081 ctgggcgacg ccctcgtctt catccagcac gcccagggcg cgggcgaact tgccgctgtt
     7141 gtcggcgaag ctccagatcg gcagcttgtg cagatcgggg tgctcgcggc gccagccgag
     7201 cttgctgtgc tcgttgtcgg tcgagccgcc cagcacgacc gtgtcgcggt cggcgaattc
     7261 cttgttcagc cgggcgaact cggcgatctc ggtcgggcag acgaaggtga agtccttcgg
     7321 atagaagaag atgaccttcc acttgccgtc gaaactggcg ttcgtgaggg tctcgaacgc
     7381 gctctcgccg ttctcctcgt gccgcatgaa gccgggcttc acgccgacga tcctgaattc
     7441 cggaagcttt tgaccaacgc cgagcatggt gggtgtctcc ttgagtgact gaggcgccgg
     7501 acgccgcggg gaggcggggt gtcgtccggc tgtgaggacg agataggcgc ggggcggcca
     7561 tagcgccaat tgatagttct ttccggatcg attgatcgag cctatgatgc cgccatgctc
     7621 cccacgatcc gccagctgca gtatctcaag ctcctctccg accacggctc gttcagccgg
     7681 gcggccgagg cggcccatgt cacccagccg acgctgtcgg ccggcatcgc cgagctggag
     7741 aagatcctcg gcgcgccggt ggtcgatcgc gcgcgctcgg gcgtgatcct cacggcggcc
     7801 ggcgaggagg ccgtgcgccg cgcgcgggtg atcctcgaac agaccgacga cctggtgcag
     7861 gcggcccagg gggccggcca gccgctgacc gggcggttcc ggctgggggt catcccgacc
     7921 gtcgcgccct tcctgctgcc gcgcgccctg ccggtgctgc gcaagcagtt ccccaagctg
     7981 cggctgttcc tgcgcgagga cctgacccat cgcctgatcg cctcgctcaa ggccggcgcc
     8041 ctcgacgcgg ccctgatcgc cctgccctac gacatgaccg gcctggcctg ggcccacgtc
     8101 gaggatgacg aactgctggc ggcggcgccc aacggccacc cgatcctcga ccagaagcgc
     8161 gtcaaccccg aggacctggc gccgaaggac ctgatcctgc tggaagacgg tcactgcctg
     8221 cgcgaccacg ccctggccgc ctgcgggctg gagccgccga agtcgaccga cgaggagacc
     8281 ttcgcggcca cctcgctgcc gacgctcgtg cagatggtcg ggtcgggcct gggggtttcc
     8341 ttcctcccgg ccatggcggt caacgccggc ctgaccgaac acgtcggcgt caccacccgc
     8401 ccgctgcagg ccgagcaccc cagtcgcgag atcgtcgtcg cctggcgcgc agggtcgagt
     8461 cgcgccaacg aggggcggat gctggcggaa gtgctgcggg gggtgtgacg tcggggacat
     8521 gtaccgcctt cggtacgtgt cccccaatcg agcggcccgg atttagggga cacgtaccgt
     8581 cggtacatgt ccccgatgtt tcgatatctt ttcgatatat ctgacttgac ggcccgacat
     8641 tccgatatat cttgtattcc gatatatcga gaaaggatcg atttatgcat cgccactttg
     8701 gcttttccca ccacaagcac gaacgcttcg gccgccacgg cttcggcgga catcacgggc
     8761 ggcacatggg tcgcggcggc cgcttcggcc ggttcttcga ccatggcgac ctcaagctgg
     8821 tcatcctgcg gatgatcgcc gaccagccgc ggcacggcta cgaactgatc aaggaagtcg
     8881 aggaacaggc cggcggcgcc tattcgccca gccccggcgt ggtctatccg accctgacct
     8941 ggctcgagga gatgggcttc gtcaccgccg ccgatgcggc cggcgggcgc aagctctaca
     9001 ccatcacggc cgagggcgag gcgcacctgc gggccaacga gggccagacg gcctcgattt
     9061 tccagcgcat ggccgaggcc gccgccgcca gcaccgcctt ctcgccgcag atcctgcggg
     9121 cgcgggagaa cctgcgcacg gcgctgaagc tgaagaccac cggcggggcg ctcagcaagg
     9181 accagatcga gtcgatctgc gacgccctcg acgcggccgc ggcggcggtc gagcgggcgt
     9241 aggggacatg ttccggcgat agccggtacg tgtcccccat ccggcgacca gggtagggga
     9301 cacgtacccg agggggtaca tgtccccggc taatccatca gccgctggct caggtaggtg
     9361 aactccaggg ccacgcgctt ggcggtctcg ttcaggttgg ccgcggccga atgcccgccg
     9421 tcgatgttct cgtagtagag gaagctgtag cccagcgcct ccagccgcgc ggcggccttg
     9481 cgggcgtggg cggggtggac gcggtcgtcc ttggtcgagg tctcgatgaa cacctgcggg
     9541 tagggcttgc cggctttcag gttgctgtag gggtcgtagg cggcgatcca ggcccgctcc
     9601 tcggggatgg ccgggtcgcc gtattcgccg acccaggagg cgccggcgcc gatgtgggtg
     9661 taggccagca tgtcgaacag tggcacctgg atgaccacgg cgttgtacag ctcgggccgt
     9721 tgggtcaggg ccacgcccat caggaggccc ccgttggacc cgcccatgat gccaaggcgc
     9781 cggggcgagg tgatcttgcg cgcgatcagg tcctcggaga cggcgaagaa gtcgtcatag
     9841 gcccgctggc ggttggcctt cagcgccgcc tcgtgccagg ccgggccgaa ctcgccgccg
     9901 ccgcggatgt tggccacgac gtagacgccg ccgcgctcga gccaaagctt gccgaccgtg
     9961 gccgagtagc ccggggtcat cgacacctgg aagccgccat aggcgtactg cagggtcggg
    10021 gcggttccgt cgtacttcag gtccttcggc cgcacgacaa agtagggcac cttggtcccg
    10081 tccttcgagg tggcccagaa ctgctccacc acatgggtcg aggcgtcgaa cctgggcggc
    10141 agctccttga tcttctcgac cttgccgctg gccgcgtcgg ccagccacag ggtgctgggc
    10201 gtcaggtagc ccgagacgtt gaagaacacc cggtcgtcac gctcggaggc cgagccgacg
    10261 ccgatggtga tattggccgg caggtccagc ttgcggccgg tccaggtcgt gggtccggcg
    10321 ccgtcgccgc cgggtgtgta gacccaggcc tggccggtga cgttctccgt caggccgacg
    10381 atcaggcggt tgcgggtgat gttgatctgt tcgaccgcct ggctgggcgt cgggcgcagg
    10441 atgagcaccg ccgacgcccg gtccggctcg ccgatcaggg actgaaggtc gatcgacgcc
    10501 agatcgccct gcttcaggct ccggctcggc cagtcctcct gcagggtgaa caccagctgg
    10561 ccgccgacca gcccctgcag tgcggtcttc atcgggaacg gcagcttgat cggaccctgg
    10621 tcggtgatca gccagtattc ggtctcgaag aagctcgggc tgcgggcggc gatcaccgcc
    10681 tgcaccttgc cgtcggcgtc gcgcagcacg aagggcgaga ccgacacgtc gtcggccgtg
    10741 ccgcgataga tctcggtcgc ctgctccagc ggcgtaccgc gcctccacag cttcagcacg
    10801 aagggatagc tcgacttggt catcgtgccg gggccccagt cgcgggcgat cagcacggtg
    10861 tccttgtcga gccaggacgc gttctgcttg ccgtcgggcg agacaaagcc gccctcgacg
    10921 aaggccttgg tcaccgtgtc gaattcgcgc agcgtgaccg cgtccttgcc gccatcggac
    10981 agctggacca ggcaatagcg gtcgtcgggg gccaggcagg tcgcgccctt gtagacccag
    11041 ttctggcctt cggccctggc cagggcgtcg acgtcgagca ccgtctccca gaccgggtcg
    11101 gccgtgcgga agctgtcgac gctggtgcgc cgccacaggc cgcgcacatg ttcggcgtcc
    11161 tgccagaaat tgcccagcgt gccgtcgccg ctgaagctga cgccggggat gcggtccctg
    11221 gcgttgacga tgtcgagcgc cgactggtgc aggccctcgt agcgggggtc gttctgcagc
    11281 acaggcaggg agcgggcgtt ctcggcccgg gcccagtcga gggcgcgggt cccctcgatc
    11341 tcctccatcc aggtgaacgg gtcggccggt gtcggggccg gctgggcgga ggcggtgaag
    11401 gcggcggcgg tcatggtcaa ggccagtacg aaggaacgca tcgtcagtcc atcagctgtc
    11461 gggtgagata ggtgtagtgc atgccccagc gccgggcgtt ggcggtcggg tcggccccgt
    11521 tggcatgacc gccgtcggtg ttctcatagt acaggtaggg cgcgcccagc tgcgccagca
    11581 tggccgcgaa cttgcgcgca tggcccggat ggacccggtc gtccttggtc gaggtggtga
    11641 tgtaggccag cgggtatttc acgccgggcc tgaggttctg gaacggcgag tatttcgcga
    11701 tccaggccgc ctcttcgggg atggtcgggt cgccgtattc gccgatccac gacgcgccgg
    11761 ccggcagcag gtggtagcgc agcatgtcga gcagcgggct ttcgacgacc accgcgttcc
    11821 acaggtcggg ccgctcggtc atggccacgc ccatcagcag gccgccgttg gagcggccgt
    11881 aggcgcccag gcgccggggc gaggtgatct tgcgggcgat caggtcctcg gcgaccgcga
    11941 aatagtcatc gtaggcccgc tggcggttgg ccttcagcgc cgcctcgtgc caggccgggc
    12001 cgaactcgcc gccgccgcgg atgttggcca ccacatacgt gccgccgcgc tccagccaca
    12061 gcttgccgac gatcgggtcg tagacgggca gcttgctgag ctggaagccg ccgtaggcgt
    12121 gcagcagggt cggattggag ccgtcgtggc tatgtccctt cctcgtgacg atgaagtagg
    12181 ggatcttcgt cccgtccttc gaggtcgcct cgtgctgctc gaccatcagg ccggaggcgt
    12241 cgaaccgggc gggcagcgcc ttgacggttt cagactgtcc cgtggctgcg tcggccagtt
    12301 tcagttcggt cggggtggtg aagccttcga ccgaatagaa tagctgatcg cccttgtccg
    12361 cggtcgcgac cagcgccgcc gagccgttgt cgatgaccgg cagggtcgcg gatttccagc
    12421 cccattcccc ccgatcgaca aaggtcatca ccgacgcgcg gacgttctca tagacgacgg
    12481 cgacaacccg atggtcggtg acggccacct ggtcgatcga gcgccgcggg gcgctggcca
    12541 gcagcaggca ggggtcgcag tcgttgccca tcaggacgcc cgccggctgc ggcttgaggg
    12601 cgtgcggcgt ggccgacatc agggagcccg cggcgaacgt gggcggcttg tcgtcgccga
    12661 cccagcgagg acgggtccag tcttcttcga tggagaagat cacgtcgccg ccaaccagcc
    12721 cgtagatccg cgacctctgc gggatcgcca gcggggttac gccgtcggcg tccagcatcg
    12781 cgtaccgggt gtggaagaaa tccagcccct gctcgagcag cacgacgcgt cgtccgtcgc
    12841 catcgcgcag cacccgcgcg gtggcgctga cgtccttcac atcgccgcgg aacacctcga
    12901 ccgcctggtc cagcgtctga ccgcgcttga gggtcttcac cacgaagccg tagccggact
    12961 cggtggtcgt gccgggaccc cagtcgcggg tcaggatcaa ggtgtcggcg tccagccagg
    13021 cgacggtctg cttgctctcg ggcaggcgga agccgccgtc gacgaaagcc tttgtcttca
    13081 ggtcgaactc gcggatctcg accgcgtcct tgccgccgtt gctgagttcg accaggcagc
    13141 ggtcatgcgt cgccgggcgg cagtcggcgc ccttccagat ccagttggcg tgttcggcgg
    13201 tggccagggc gtccaggtcg agcaccgttt cccactgcgg gtcggccgtg cggtagctgg
    13261 ctagcgaggt gcgccgccag acgccgcgca catgggtcgc gtcctgccag aagttgaaca
    13321 cctggtcgcc gacgaaggtc ggggtggcga tgcggtcgct ggccgacagg atcttgaggg
    13381 tctcggcctg caggccggcg aaccgcgcgt cgccggtcag cacgggcagg gtcttcgcgt
    13441 tttccgcccg cacccagtcc atcgaccggt cgccgtcgac ctgctcgagc cattcgaacg
    13501 gcgtctcggt ggcggccatc accggcgcgg cgaccagcac ggcggcggcc gcgagaaagg
    13561 cggaaaattt catcggagac cccggcatct ggacagccgg cgagcatagg ttgacgcgag
    13621 ggtcaggcaa cccccgggga gattacgggt ctgtaagaat ccggcgcgcg tgggcttcgc
    13681 gtcgaagcgt cctttggcgc gcgtttacaa aggtcctata tctgcgccga catgacccgc
    13741 ccctttctca agatgaacgg cctgggcaac gacttcgtcg tggtcgaggc gcggggcgag
    13801 cccttccggc cgacgccggc cgaggcgcgc gccatcgccg accgggaaag cggcgtcggc
    13861 tgcgaccaac tgatcggcat cgagccgacc cgtcgcgccg acgccttcat gcgcatctgg
    13921 aacgccgacg gcggcgaggt cgacgcctgc ggcaacgcca cccgctgcgt cgcctggctg
    13981 ctgatggagg cgaccggtcg cgacgaggcg gtcatcgaga ccgaggccgg cctgctnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnggacac caagggcatc gagctgcagg tcggcccgat
    14161 cgacgcgccg cacctgcaca cgcccggcgc ggtcagcatg ggcaatcccc atgtggtgtt
    14221 cttcgtcgac gcggagccga ccgacgccct ggtcagggga tcgggcagcc tgatcgagca
    14281 tcacccgttg ttccccgagg gggtcaacgt cggcttcgcc tgggtcaagg cgcccgaccg
    14341 catccgcctg cgcgtctggg agcgcggcgc cggcctgacc aaggcctgcg gcacgggcgc
    14401 ctgcgcggcc ctggtcgcct gcgcccgccg cggcctgacc ggccgctcgg cgaccatcga
    14461 gatggacggc ggcgagctgc aggtcgagtg gcgggccatg tgatcatgac cggcccggtg
    14521 gcgctggaat tcaccgggcg gctttagtcc tacgccggcg gagggtacgg cggcgctcca
    14581 gagatcgcga cagttacatt cgttgcgaac caaatgacga ttccaaccat ggccacgacc
    14641 agcgctaggc gaatgataac tagcagcgct cgctgaaagc cgccgccagt acgccaagcc
    14701 tcgggcccga aaacgaagct caggtattgc gcatgctgcg agggcgtgag cgggactatc
    14761 ggaaaggatg gtccagccct cttcgttatt gaaactaggg cgctagcgat caagagcgtc
    14821 atgaccgcaa ggcttacgga cgcgccaatc aggtccccat agggagcgat gactttcgat
    14881 aggcccagcc aacgattcat gcgccgtcct tccgctagcc gaacttgaac ccgcagagct
    14941 tattaccgtc caggtcgcgg aaataggcga aataggcgcc cggctggcgc tcgccgggag
    15001 gcccctcgca ggtcccaccc agctcaagcg ccttcgcgtg gaacgcgtcg acctcctcgc
    15061 gactgtcgaa gctgaagccg cccatcatgc cgttgccgac gctcgccgcg ccgccgtcgt
    15121 aggggcccag cacgccgaac agacccgtgg ccttgccgcg atagagccgt ccgccggtcg
    15181 gatgctcgaa gacgcccttc atgccgatcg agccgagcag ggcgtcgtag aagcccttgg
    15241 cggcctccag atcgttcgat ccgacggtga tgtagctgac gctggccatg acctgtctcc
    15301 caagtccgtt gcatttagaa atcgacctct agcatggctg tcggttctgt gaagccatcc
    15361 gcgcgcagtg acccgttttg aggaaaccgg cgttccgcca ccggcggtga ggggctaggc
    15421 tccggccgac cttgaccggc gcgccgatcc cggacgcgcg gggacccgat cgaacggagc
    15481 cgccatgtcc agattcactg tcctcgccgc catcggggcg cttgccgtcg ccacgtccgt
    15541 cgccgtcccg atgccggccc gcgccgagag cgcggcggtg tcggccacct gcaacacgct
    15601 ctacacgacc tcgacggcgg tgctgggaac cgccgcgacg gtcggtccgg ccagcgtcgg
    15661 ctatccgggc caggccagcg cccagggctg ccagatcgcc ttctccggcg acgggacgca
    15721 gtacgggacc aacttccagg ccgtggccga caagctcgac gcgatgatgc tgggcgcggg
    15781 ctggacccgc gatcacaacg ccgacgccga cggccccacg ggcacggcga cgggctacaa
    15841 gaagggcggc gagctggtcg ccgtcagcgt cggctacgac acgcccgccg gagtctgccg
    15901 caaggatgag cccagcgcca gctgccatcc gacggccgcg cagatgctct acaccatcac
    15961 cctgggggtc gtggccgccg gctcctgacc gcgcgtcaga ggctgtattc ccgctcaagc
    16021 acatagcgcg agccctcgcc ggtctggtgg ctcgaataga gaccgaaccg gtcgatcgcg
    16081 aacggcggcg accgcagcag gttgttggcc tggatccaga ccgccacgtc ggtcggatcc
    16141 gggcgtcgca gataggccag cgtcagatga gggcgccagg tccgaccgtc gggcgtgacc
    16201 cgtgcgcgtc gcgccgccgt ctcgcagcgg ccggcgagcg ttcgcagggg cgggttctcg
    16261 gccaccccgg cccagaccgc atggatgtcc ggcccctcac cgaacgcgcc gacgccgtcc
    16321 agcgtgaccg cgaacggcgc gctggtgatg tgggacagtt ccaggtcgag gtcctcggcg
    16381 accctttcct gcacgtcgcc gaaaaaccgc agggtgatgt ggaaagcctc cagcggccgc
    16441 cagcgcgcgc ccggaagccc ctgctgccgg cgcaacagcg gctcgccgat ctcctcggga
    16501 atggcgatcg cggcgaacag gcggatcatc gggccttcgc gagcgccctg gccacgaccg
    16561 gcgccagccc cgcggcgatg atcctgaccc cggcggcgtt gggatggatg ccatcgcgct
    16621 gattcagccg gggatcgccg gccaccccgg ccagcaggtt gggatacagc ggcacgccgc
    16681 gttgcctggc gagggtcgga aacacggcgc cgaactcccg cgcgtagttg cggccgatgg
    16741 cggtgggcgc gctgaggccg gccagcacga cggtgatccc gcgcgcctgc aggcggcgga
    16801 tgatgcggtc gaggttggcc cgcgtctgct tcgggtcgag tccctgcagc aggtcgttgc
    16861 cgcccagggc cacgacgcag acggccgtat ccttctggac gctgaagtcg acgcgcgccg
    16921 cgccgccggc gctggtgtcg ccgctgaccc cggcgctgcg cacccgaacg ccggggacca
    16981 gtcgcgtcag cgcgacctcc agctgcgcgg ggaggg
//
LOCUS       DAORNX010000009        16416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2689_length_16416_cov_7.379867, whole genome shotgun sequence.
ACCESSION   DAORNX010000009 DAORNX010000000
VERSION     DAORNX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_16416_cov_7.379867"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="PLO65_00810"
     CDS             complement(<1..1883)
                     /locus_tag="PLO65_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRD26819.1"
                     /translation="MTKKMLIDAAHPEETRVVVVDGTRVEEFDFESQSKKQLRGNIYL
                     AKVTRVEPSLQAAFIEYGGNRHGFLAFNEIHPDYYQIPLADREALMRAQAEEDDEPVA
                     GNGRGRAAAAESDDDDDHEVSEDEDDVMDEEVARRRRRLMRNYKIQEVIRRRQIMLVQ
                     VVKEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITTVTDRKKLKTIVQSLDV
                     PKGMGLIVRTAGAKRTKTEIKRDYDYLLRQWENIRETTLHSIAPALIYEEEDLVKRAI
                     RDLFDKDIDGVWVEGERGYKDARDFMRMLMPSQAKKVQLYREPTPLFVAHKVEGHLSQ
                     IYTPVVPLKSGGYLVINQTEALVAIDVNSGKSTRERNVEATAVKTNMEAAEEAARQLR
                     LRDLAGLVVIDFIDMEESKNNRAVEKKLKDSLKDDRARIQMGKISSFGLMEISRQRRR
                     SGVLEGTTHVCEHCAGTGRVRSPESSALAALRAVEMEALHGGGEVTLKVSPAVGLYIL
                     NEKRAYLTRVHQTHHLFVTVQIDAALGQAEHEIERTASGEHSLHDLEPVPTVGRVAYD
                     PEDDLLDDGSDIEDEEDEDEDEDEEEAEENEGGLAGDDGDDDEDARPARAARGEGESG
                     DEAG"
     gene            2304..3500
                     /locus_tag="PLO65_00815"
     CDS             2304..3500
                     /locus_tag="PLO65_00815"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD26820.1"
                     /translation="MRALATGGVLAGWRGYFALACLILTVIALGGVARGSSNSGLLKV
                     RLGGDQAETRIVLDLDRSVTGKLVSDGADGKVVVALPRIDTSGDLSGGGQGLVRRWNL
                     ERGPTALKLHIDLVRDAEVRRRFLLPPGDGVSTYRYVIDLKAIGEAPAAAPARRTTSP
                     PLPTTTRAMARSGPRVVVIDAGHGGKDPGAPGAASHEKDVTLAAAKALKARLERTGRY
                     RVVLTRSTDAYIPLDSRVRIARSADADLFISLHADSGPTTAMRGASVYTLSERGESRV
                     TSVLRKDDWLMPVNFPGADRTVGEIILDLTQRSTRNRSAAFAEVLLEHIADETTLLRR
                     SHRDGNLMVLLAPDVPAVLLEMGFINNPEDERQLTDSASRRRLMDAVGDAVDAWFARQ
                     VKTASR"
     gene            3557..5995
                     /locus_tag="PLO65_00820"
     CDS             3557..5995
                     /locus_tag="PLO65_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRD26821.1"
                     /translation="MLEKALLRLWPFEEPPERWVAIVGVSVLSLIAVAGFTLAIYAAW
                     LFHDMPDAGDLTDYRPPTATRVYAWDGTLIGEFSRERRIFIPYDQLPPRLVQAFLAAE
                     DRNFFQHSGVDVQGLSRAMIKNVLNALQGRRLEGGSTITQQVAKNVLLTSDATVGRKL
                     KEAILARRLEQTLTKDRILELYLNEIWLGYRSYGVGAAAFNYFGKPVSELTLAECAYL
                     ASLPKGPDNYHPIRRKANAIARRNWVLSQMAELGWVSRADAEAAMKEDLKVQAAPSRA
                     KYKDADYFVEEVRRRGLATLGPRLSEGGYYMRTTLDPRLQTAARVALMDGLENYDRRH
                     GWRGAWGTTNMAAGWEKAALKKSPPSERRGWRAAVVASVTGGVRVVPARELPPGNLVA
                     EDVAWARAGKGLKVGDLVFVEPASSGGYRLRQVPAVNGALVAIEPHSGRVLAMVGGYS
                     FSLSSFNRATQAMRQPGSAIKPFVYAAALEPDGGYTPASYVTDAQISFKGAVKGEDWS
                     PQNYNKKYYGSMPLRRGLELSRNAMTVRLAQGVGMKKIADLCVKFGIVRSMQPVLAMA
                     LGAGETTPFKLTAAYTAFVNGGRKVEPHLIELVQDREGQVIYRADKRDCPRCTAGFGG
                     EESPRVAPAGEQIIDPITAYQISSMLEGVVQRGTATPARVLGRPVGGKTGTTNEYRSA
                     WFVGFTPQVVVGVFVGFDDNRSLGEGEVGGVSALPVFIDFMTEAVKGMPVEAFTPPKA
                     AKFAVVGGQREAFRPGTEPRGPVGPVAPVNPAGPQNYQEAWPGGKLSTEQSPSQPPKP
                     APKPRIPDDLSGLY"
     gene            6042..7164
                     /gene="prfB"
                     /locus_tag="PLO65_00825"
     CDS             join(6042..6110,6112..7164)
                     /gene="prfB"
                     /locus_tag="PLO65_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480453.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRD26822.1"
                     /translation="MRPDVEAAKADIEQSVGLLRRRLDWDAALRRLHELDARSEDPTL
                     WDRPSEAQALMRERQQLADRVEAVRAIERDLSDALEYAEMADAESDEASLEDARAQLK
                     DIRARAAKAELEALLSGEADGNDAYVEINSGAGGTESCDWANMLLRMYTRWATAHGYS
                     VELVEETSGDQVGIKSATLQVKGPNAYGWLKTEAGVHRLVRISPYDAAAKRHTSFASA
                     WVYPVIDDTIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIAVACQAGR
                     SQHQNREEAWKMLRARLYEMELQKREAAAQALEDQKTDIGWGHQIRSYVLQPYQMVKD
                     LRTNVETSDTDGVLNGDLDAFMGAALAQRVGATRDAVEG"
     gene            complement(7374..7862)
                     /locus_tag="PLO65_00830"
     CDS             complement(7374..7862)
                     /locus_tag="PLO65_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRD26823.1"
                     /translation="MFSKQTKTPPKPTAASAPAPAPTPQAPEPPKARPKVASLISDGI
                     TIEGGVVGDGELQIDGVVRGDVRVGRLTVGDTGHIEGSVYAEAAEVRGRVIGSVTAKQ
                     VRLYGTAYVDGDITHEQLAMETGAFFQGRSLKFQRPQQPQHSPAPQAPHPQSLGIPPK
                     VG"
     gene            complement(7843..8994)
                     /locus_tag="PLO65_00835"
     CDS             complement(7843..8994)
                     /locus_tag="PLO65_00835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRD26824.1"
                     /translation="MATARLRRIRRALEELFPERHLYIRSGGEMRGFVLSTGKQIAGA
                     GLIGAAALWMGVCTAAMLVSALSVSATDQELARRNAYWERVTADRQARLNSAMQQLSQ
                     TDGSLGELASSVEKRHAALALLLTEFKGAPGAMAALAPAKPRLNAGSPVQRVQYTLMD
                     QERMISAAESYALSRAERLRLAFRLAGLNPDSYAARGGGLGGPLIEARDPRALAAVLD
                     VDESFAQRIQRASSNMSDMRSLATAARGLPFFRPTSAAAQSSSYGVRLDPFTRRPAFH
                     SGLDFPGASFTPIYATAPGVVSFTGVRSGYGNTVEIDHGSGFKTRYAHLAGYTVSVGQ
                     RVAVGQRIASMGSTGRSTGPHLHYEVWVNGRAQNPNRFLKAGEYVQQAD"
     gene            complement(9272..9736)
                     /locus_tag="PLO65_00840"
     CDS             complement(9272..9736)
                     /locus_tag="PLO65_00840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD26825.1"
                     /translation="MTDLTIGDRAPDFDLPTDTGRVSLSALKGKTVVLFFYPKDDTPG
                     CTTENIAFSGLKAEFDAAGAVLVGVSKDTAAKHGKFRAKHGLTVELGSDADSDVIERY
                     GAWVQKSLYGREHMGIDRSTWLIDGEGVIRKVWRKVKVKGHAEEVLAAARAH"
     gene            complement(9906..11180)
                     /gene="tyrS"
                     /locus_tag="PLO65_00845"
     CDS             complement(9906..11180)
                     /gene="tyrS"
                     /locus_tag="PLO65_00845"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRD26826.1"
                     /translation="MTADAAFKSDFLRTLQDRGYIHQITHPEELDAAATQGVVTAYIG
                     FDATAPSLHVGSLIQIMMLRRLQQAGHRPIVLMGGGTTKVGDPTGKDESRKLLSEADI
                     RANIASIKTVFEKFLTFGDGPTDAVMVDNDEWLSKFGYVEFLREFGVHFTINRMLAFD
                     SVKQRLEREQPMTFLEFNYMLMQSVDFLELSRTRNCRLQMGGSDQWGNILNGVELIRR
                     VDQKPAFGLTTPLLSTASGAKMGKTAQGAVWLNADMRSPYDYWQFWRNAEDADVGRFL
                     RLFTDLPLDEVARLEALEGAAINDAKKVLADEATRMLHGADAARAARAKAEEAFELGN
                     TAAAATSVDFLWAGENGDLTQRSAEAIVKGAGLAGSVKEARNLIEQGTILINDVRITN
                     PKAHYNRTDGTGDDGLFKIQKGKNKIVWVRPV"
     gene            11453..11992
                     /locus_tag="PLO65_00850"
     CDS             11453..11992
                     /locus_tag="PLO65_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HRD26827.1"
                     /translation="MLLALTCHPDTPCDAVSRIEVNLARGGGASLALRYVTSGDVSRI
                     SLPGRAERGRADGLWKTTCFEAFVQIPGGYAEINLSPSDQWAAYGFAGYRDGAMAQLP
                     GAALRHMTQRAGEGLYELVATLDLAGLDLPVDQPWRLGLSAVIEEASGRKSYWALAHG
                     PDKPDFHHPDSFACVLEPS"
     gene            11989..13191
                     /locus_tag="PLO65_00855"
     CDS             11989..13191
                     /locus_tag="PLO65_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRD26828.1"
                     /translation="MNFGIDRLLADPALRAPLAGKRVALLAHPASVTADLTHSIDALA
                     ACPDIQITAAFGPQHGMRGDLQDNMMESPDFTDPVYGFPVFSLYGETRRPTGQAMGTF
                     DVILVDLQDLGCRIYTFITTLLYVLEAAAEHGKEVWVLDRPNPAGRPIEGLTLLPGWE
                     SFVGAGPLPMRHGLTLGELGQWFVRSRSLDVVYRVIAMEGYDPAAGPGYGWPLGERSW
                     INPSPNAPNLSMARAYAGTVMLEGTHLSEGRGTTRPLELFGAPDIDARKVIAEMNALA
                     PQWLNGCRPRDIWFQPTFHKHVGQLCNGVQIHVDDPAYDHAAFRPWRLQALAFKAIRR
                     LYPDYRLWRDFPYEYVFDKLAIDVINGGPGLREWVDDPAAMPDDLDALATPDEQAWEA
                     ARRPFLLY"
     gene            13197..13739
                     /locus_tag="PLO65_00860"
     CDS             13197..13739
                     /locus_tag="PLO65_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRD26829.1"
                     /translation="MTMRIALLRGVNVGGAKKIAMAELKAMVEALGFTDVKTLLQSGN
                     VVFEAGERSDAELEALLETEAERRLGLVTRFIVRGPKAWRMMIDANPMPTAAEREPSR
                     FLVNVTREPVTPEMLAMIRAAATPGERIEAGDRCVYAFFGDGVADSKAALVFGKKALG
                     LTSTGRNWNTVQKIAALIGA"
     gene            complement(13774..13974)
                     /locus_tag="PLO65_00865"
     CDS             complement(13774..13974)
                     /locus_tag="PLO65_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26830.1"
                     /translation="MPASVTVGVLCAVAALLAAKAGAWGLFQRKVMKETSGLLARRAE
                     VVLAAAGLLAALAVALLVTRPF"
     gene            complement(13979..14515)
                     /gene="ppa"
                     /locus_tag="PLO65_00870"
     CDS             complement(13979..14515)
                     /gene="ppa"
                     /locus_tag="PLO65_00870"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRD26831.1"
                     /translation="MNLDAIAIGRNPPEDVNVIIEVPIGGEPIKYELDKDAGVLVVDR
                     FLYTAMRYPGNYGFIPHTLSGDGDPVDVIVVNTRAIAPGAVMSCRIVGVLLMEDQAGI
                     DEKLIAVPSSHLTARYDAVKDYTDLPRITLDQIEHFFTHYKDLEPGKWVKVKGWGDAA
                     EARRMVLEGIERAKAEKA"
     gene            complement(14535..15185)
                     /locus_tag="PLO65_00875"
     CDS             complement(14535..15185)
                     /locus_tag="PLO65_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD26832.1"
                     /translation="MPEVVLTGAAGRIEGRYSPGKRENAPIALILHPHPRAGGHMNNP
                     VAVQLYHLFMKRGFSVLRFNFRGVGRSQGEFDAGIGELADAATALDWLQATNPQASQF
                     WVAGYSFGAYIGMQLLMRRPETDGFISVSPPTNMYDFSFLAPCPASGLILHGQADTVV
                     PPVEVERVVSKLRTQKGIVIDYELVEGATHFWAEHLPEVEKRVGAYLDKRLEAEPA"
     gene            15338..15787
                     /locus_tag="PLO65_00880"
     CDS             15338..15787
                     /locus_tag="PLO65_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRD26833.1"
                     /translation="MRLSTKGRYAVMAMTDLAGRQGADDSLRAVSLAEIAARQQISLS
                     YLEQLFARLRRKGLVRSVRGPGGGYRLAQSASETNIADIVLAVDEPLRATRCGKPVGR
                     GCMVGGERCLTHDLWDEMGRQLQAYLASVTLADVLSGKLRVAASEAA"
     gene            15784..>16416
                     /locus_tag="PLO65_00885"
     CDS             15784..>16416
                     /locus_tag="PLO65_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD26834.1"
                     /translation="MTLYLDYNATAPVRPEAKAAVMRALEIGGNPSSVHAAGRAARSL
                     IEAARAQVADLIAVKPGSITFTSGGTESNALAIESAVAAGVDRILLGATEHGAVSENV
                     KATGVPVEVWPVDADGVADLAWLADALGKGGRPLVCLMLANNETGVIQPVAEASALVR
                     EAGGWLHVDAVQAAGKIAIDFNALGADTLALAAHKLGGPFGVGALAAGPRA"
BASE COUNT         2510 a         5550 c         5685 g         2671 t
ORIGIN      
        1 ccggcttcgt cgcccgactc gccttcaccg cgggccgcac gggccggtcg ggcgtcttcg
       61 tcgtcgtccc catcgtcacc ggcgagaccg ccctcgttct cttcggcctc ttcctcgtcc
      121 tcgtcctcgt cctcgtcttc ctcgtcctcg atgtccgagc cgtcgtcgag cagatcgtcc
      181 tcgggatcat aggcgacgcg gccgacggtc gggaccggct ccaggtcgtg cagcgaatgc
      241 tcgccgctgg ccgtacgctc gatctcatgc tccgcctggc ccagggccgc gtcgatctgc
      301 acggtgacga acaggtgatg cgtctggtgc acgcgggtca ggtaggcgcg cttctcgttg
      361 aggatgtaca ggccgacggc cggcgagacc ttcagggtca cctcgccgcc gccatgcagg
      421 gcttccatct cgaccgcgcg aagggcggcc agggcgctcg attccggcga gcgcacgcgg
      481 ccggtgccgg cgcaatgctc gcagacgtgg gtggtgccct ccagcacgcc gctgcgacgg
      541 cgctggcggc tgatttccat caggccgaac gagctgatct tgcccatctg gatgcgggcg
      601 cggtcgtcct tgaggctgtc cttcagcttc ttctcgaccg cccggttgtt cttcgactcc
      661 tccatgtcga tgaagtcgat gaccaccagg ccggccaggt cgcgcaggcg cagctggcgg
      721 gcggcctctt cggccgcctc catgttggtc ttgacggccg tggcctcgac gttgcgctcg
      781 cgcgtggact tgccggagtt gacgtcgatg gcgaccagcg cctcggtctg gttgatcacc
      841 aggtagccgc cggacttgag cggcacgacc ggggtgtaga tctgcgacag gtgaccttcg
      901 accttatgcg ccacgaacag cggcgtgggc tcccggtaca gctgcacctt cttggcctgg
      961 gacggcatca gcatgcgcat gaagtcgcgc gcgtccttgt agccgcgctc gccctcgacc
     1021 cagaccccgt cgatatcctt gtcgaacagg tcgcggatag cgcgtttgac gaggtcttcc
     1081 tcctcgtaga tcagcgccgg agcgatcgag tgcagcgtcg tctcgcggat attctcccac
     1141 tggcgcagca ggtagtcgta gtcgcgcttg atctcggtct tggtgcgctt ggcgccggcc
     1201 gtgcggacga tcaggcccat gcccttgggc acgtccaggc tctggacgat ggtcttgagc
     1261 ttcttgcggt cggtgacggt ggtgatcttg cggctgatgc cgccaccgcg cgcggtgttg
     1321 ggcatcagca cgccgtagcg gccggccagc gacaggtagg tggtcagggc cgcgcccttg
     1381 ttgccacgct cttccttgac gacctgcacc agcatgatct gccggcggcg gatcacttcc
     1441 tggatcttgt agttgcgcat caggcggcgg cgacgacggg ccacctcctc gtccatcacg
     1501 tcgtcttcgt cctcggagac ctcgtgatca tcgtcgtcat ccgattcggc cgccgcggcg
     1561 cgaccgcgac cgtttccggc gaccggttcg tcgtcctcct cggcctgggc gcgcatcagc
     1621 gcctcgcggt ctgcaagagg gatctggtag tagtcggggt ggatctcgtt gaaggccagg
     1681 aagccgtggc gattgccgcc gtattcgatg aaggccgcct gaaggctggg ttcgacccgc
     1741 gtcaccttgg caaggtagat gttcccgcga agctgtttct tggattggct ttcgaaatcg
     1801 aactcttcaa cccgggttcc gtccacgacg acgacccggg tttcttccgg gtgagcggcg
     1861 tcgataagca tcttcttggt catttaaagc gtctccccgg cgcgcggcgg gcgcttggcc
     1921 cggtcgccgg catgatgtga aggcgcgctc cgtgtggccg tcctgcgcgc cgcgagcggc
     1981 ggtgcgtcgg ggcgaaagcc aggccagggc gtgtgcgcag cccatgaggt ctattccctg
     2041 tcgcgccagg atcggcgcgg gatcagatgg tcgcgtcgtt cgccggaggg cgatgggccg
     2101 gacgcacggc ctttcattct gtctcatgcg cggagaatcc ccgtcagtcg cggggaccgg
     2161 cgcgcgggac gacattacgc ttatccgcgc gcaaaaggaa agcgcgtcgg cgctcgccgc
     2221 cgtaggcaaa gccttaacca attcgacacc cgagacgggc gatcatggcc gtcaaaatcg
     2281 caaagcgatg actggggcgt agaatgcgag cgttggcgac cggtggagtt ctggcgggat
     2341 ggaggggcta tttcgccctg gcctgcctga ttctgacggt cattgctctc ggcggggtcg
     2401 cccgcggctc gtccaactcc ggcctgctca aggtgcgcct cggtggcgac caggccgaga
     2461 cccgcatcgt tctggacctc gaccgctcgg tgaccggcaa gctggtgtcc gacggtgcgg
     2521 acggcaaggt cgtggtcgcc ctgccgcgga tcgacacctc cggcgacctg agcggcggcg
     2581 ggcagggtct ggtgcgccgc tggaacctcg aacggggccc gaccgccctg aaactgcata
     2641 tcgatctggt ccgtgacgcc gaggtgcgcc ggcgcttcct gctgccgccg ggcgatggcg
     2701 tctcgaccta tcgctatgtc atcgacctga aggcgatcgg cgaggctccc gccgcggcgc
     2761 cggcgcggcg gaccacgtcg ccgccgctgc ccacgacgac ccgcgccatg gcgcgcagcg
     2821 gtccgcgcgt cgtggtcatc gacgccggcc acggcggcaa ggacccgggc gccccgggcg
     2881 ccgcgtcgca cgagaaggac gtgaccctgg cggccgccaa ggcgctcaag gcccgactcg
     2941 agcggaccgg ccgctaccgt gtcgtcctga cccgctcgac cgacgcctac ataccgctcg
     3001 acagccgcgt gcggatcgcc cgatccgccg acgccgatct gttcatctcg ctgcacgccg
     3061 attccggtcc gacgaccgcc atgcgcggcg ccagcgtcta caccctgtcc gaacgcggcg
     3121 agagccgggt gaccagcgtg ctgcgcaagg atgactggct gatgccggtc aacttccccg
     3181 gcgccgaccg cacggtgggc gagatcatcc tcgacttgac gcagcgctcg acccgaaatc
     3241 ggtcggccgc tttcgccgaa gtgctgctcg aacacatcgc cgacgagacg accctgttgc
     3301 gccgcagcca ccgcgacgga aatctgatgg tgctgctggc ccccgatgtg ccggccgtgc
     3361 tgctggaaat gggcttcatc aacaaccccg aggacgagcg gcagttgacc gattccgcca
     3421 gtcgcagaag gctgatggac gccgtgggcg acgccgtgga cgcctggttc gcccgtcagg
     3481 tgaagaccgc gagccgctga ccgatctctg acgtcggggc cgttggcgcc cggctccagt
     3541 caggttaagc aggcccatgc ttgagaaggc ccttctgcga ctttggccgt tcgaggagcc
     3601 gcccgagcga tgggtggcca tcgtcggggt cagtgtgctc agcctcattg cggtggcggg
     3661 cttcaccctg gccatctatg cggcctggct gttccacgac atgcccgatg cgggcgacct
     3721 gaccgactac cgtcccccga cggcgacgcg ggtgtatgcc tgggacggca cgctgatcgg
     3781 cgaattctcg cgcgaacggc ggattttcat cccctacgac cagcttccgc cgcgtctggt
     3841 gcaggcgttc ctcgccgccg aggaccgcaa cttcttccag cacagcgggg tggatgtgca
     3901 gggcctgtcg cgggccatga tcaagaacgt gctcaacgcg ctgcagggcc ggcgacttga
     3961 aggcggctcg acgatcaccc agcaggtggc caagaacgtc ctgctgacga gcgacgccac
     4021 ggtcgggcgc aagctcaagg aagcgattct cgcccggcga ctcgaacaga ccctgaccaa
     4081 ggaccggatt ctcgagctct atctgaacga gatctggctc ggctaccgct cctacggggt
     4141 cggcgcggca gccttcaatt acttcggcaa gccggtgtcg gagctgaccc tggccgagtg
     4201 cgcctatctg gcgtcgctgc ccaaggggcc ggacaactac cacccgatcc gccgaaaggc
     4261 gaacgcgatc gcccggcgaa actgggtgtt gagccagatg gccgaactgg gctgggtcag
     4321 ccgcgccgac gccgaggcgg cgatgaagga ggacctcaag gtccaggccg ccccgtcgcg
     4381 ggccaagtac aaggacgccg actatttcgt cgaggaggtg cgccggcggg gtctggccac
     4441 cctcggcccg cgcctcagcg agggcggcta ctacatgcgc accacgctcg atccgcggct
     4501 gcagaccgcc gcgcgcgtgg cgctgatgga cgggctggag aactacgatc gccgccacgg
     4561 ctggcgcggg gcctggggca cgaccaacat ggcggcgggc tgggagaagg ccgcgctgaa
     4621 aaagtcgccg ccgtccgagc gccgcggctg gcgcgccgcc gtggtggcca gcgtgaccgg
     4681 tggcgtccgc gtcgttccgg ccagggaact tccccccggg aacctggtcg ccgaggatgt
     4741 cgcctgggct cgcgcgggca agggtctcaa ggtcggggac ctggtgttcg tcgagccggc
     4801 ctccagcggc ggctatcgcc tgcgccaggt gcccgccgtc aacggcgccc tggtggccat
     4861 cgagccgcac tcgggccggg tgctggcgat ggtcggcggc tattccttct cgctgtcgag
     4921 tttcaaccgc gcgacccagg cgatgcgtca gcccggatcg gcgatcaagc cgttcgtcta
     4981 cgccgcggcg ctcgaacccg acggcggcta caccccggcc agctacgtga ccgacgccca
     5041 gatcagcttc aagggcgcgg tcaagggcga ggactggtcg ccgcagaact acaacaagaa
     5101 atattacggc tcgatgccgc tgcgccgggg gctggagctt tcgcgcaacg ccatgaccgt
     5161 ccgcctggcc cagggcgtgg gcatgaagaa gatcgccgac ctgtgcgtga agttcggcat
     5221 tgtccgctcg atgcagcccg tgttggccat ggcgctgggg gcgggggaga ccacgccgtt
     5281 caagctgacc gcggcctaca cggcctttgt caacggcggc cgcaaggtcg agccgcacct
     5341 gatcgagctg gtccaggacc gcgagggaca ggtcatctac cgcgccgaca agcgtgactg
     5401 cccccgctgc acggccgggt tcggcggcga ggagagcccg cgggtcgcgc cggccggcga
     5461 gcagatcatc gacccgatca ccgcctatca gatttcctcg atgctcgagg gcgtggtcca
     5521 gcgcggcacg gccaccccgg ccagggtgct gggtcgcccg gtcggcggca agacgggcac
     5581 gaccaatgaa taccgcagcg cctggttcgt cggtttcacg ccccaggtgg tcgtcggggt
     5641 gttcgtcgga ttcgacgaca accgctcgct cggcgagggc gaggtggggg gcgtctcggc
     5701 cctgccggtg ttcatcgact tcatgaccga ggcggtcaag gggatgcccg tcgaggcctt
     5761 caccccgccg aaggcggcca agttcgccgt cgtcggcggc cagcgcgagg ccttccggcc
     5821 cggcaccgaa ccgcgcggtc ccgtgggtcc ggtggccccg gtcaacccgg ccggcccgca
     5881 gaactaccag gaggcctggc ccggcggaaa gctgagcacc gagcagtcgc cgtcgcagcc
     5941 ccccaagccc gccccgaagc cccggatccc cgacgacctg tcgggattgt attgaggcgg
     6001 tcgctgcggt aagccccgcg cccgcttgat cggagacttt catgagaccg gatgtcgagg
     6061 ccgccaaggc cgacatcgag cagtccgtcg gactgctcag gaggcgtctt tgactgggat
     6121 gcagccctaa gacgcctgca cgaactggac gcccgttcgg aggatcccac gctgtgggac
     6181 cggccgagcg aggcccaggc cctgatgcgc gaacgccagc agctggccga ccgggtcgag
     6241 gcggtgcgcg cgatcgagcg cgacctgtcg gatgccctcg agtacgccga gatggccgat
     6301 gcggagagcg acgaggcgtc gctcgaggac gcgcgcgccc agctgaagga catcagggcc
     6361 cgggccgcca aggccgagct ggaagccctg ctgtccggcg aggccgacgg caacgacgcc
     6421 tatgtcgaga tcaattccgg cgccggcggc acggagtcct gcgactgggc caacatgctg
     6481 ctgcgcatgt acacgcgctg ggccaccgcc cacggctaca gcgtcgaact cgtcgaggag
     6541 accagtggcg accaggtcgg catcaagtcg gcaacgctgc aggtcaaggg ccccaacgcc
     6601 tatggctggc tgaagaccga ggccggcgtg caccgactgg tgcgcatcag tccctatgac
     6661 gccgccgcca aacgccacac cagcttcgcc tcggcctggg tctatccggt catcgacgac
     6721 accatcgaga tcgagatcaa cccggccgac gtgcgcaccg acacctatcg ggccagcggg
     6781 gccggcgggc agcacatcaa caagaccgac tcggccgtgc gcctgacgca catcccgacc
     6841 ggtatcgccg tggcctgcca ggccggccgc agccagcacc agaaccggga agaagcctgg
     6901 aagatgctgc gcgcgcggct ctacgagatg gagctgcaaa agcgcgaggc ggcggcccag
     6961 gccctcgagg accagaagac cgacatcggc tggggccacc agatccgcag ctacgtcctg
     7021 cagccctacc agatggtcaa ggacctgcgg accaacgtcg agacctccga caccgatggc
     7081 gtgctgaatg gcgacctgga cgcgttcatg ggcgccgccc tggcgcagcg cgtcggcgcc
     7141 acgcgggacg cggtcgaggg gtagggccct tttcgccgcg cgggaggacg tcggccgacg
     7201 cggcttcagc ctgcgtcttg cgacacgccg cttcgcggct gctcacgatg acgtggattt
     7261 gccgacgtca tggtgaacgg cgccgcaggc gcgtgtgaaa ccacgcaacg tccccgcacc
     7321 gtcggctccg ccgacgtccc tccccatgaa ggggagagat cgatggcgcg aaatcagccg
     7381 accttgggcg gaatgccgag gctctgcgga tggggggcct gcggcgcggg gctgtgctgc
     7441 ggctgctgcg gacgctggaa cttcaggctg cggccctgga agaaggcccc cgtttccatc
     7501 gccagctgtt cgtgggtgat gtcgccgtcg acgtaggctg tcccataaag acgcacctgc
     7561 ttggcggtca ccgagccgat cacgcggcca cggacctcgg cggcttcggc atagaccgaa
     7621 ccctcgatgt gaccggtgtc gccgacggtc agtcggccga cgcgcacgtc gccgcggacc
     7681 acgccgtcga tctgcagttc gccgtcgccg acgacgccgc cctcgatcgt aatcccgtca
     7741 ctgataaggg acgccacctt cgggcgcgcc ttgggcggct cgggcgcctg gggcgtcggc
     7801 gcgggcgccg gagccgaagc ggcggtgggt ttgggcgggg tcttagtctg cttgctgaac
     7861 atattcgccg gccttcaaga atcggttggg gttctgcgcc cggccattga cccacacctc
     7921 atagtgcaaa tgtgggccag tcgaccggcc ggtcgacccc atcgatgcaa tacgctggcc
     7981 aaccgcaacc cgttgtccga cgcttactgt gtagccggcg aggtgggcgt agcgggtctt
     8041 gaacccgctg ccgtgatcga tctcgacggt attgccgtaa ccggaccgaa cgccggtgaa
     8101 ggagaccacg cccggcgcgg tcgcatagat gggcgtgaag ctcgcgcccg ggaagtcgag
     8161 gccggaatgg aaggccggcc gccgggtgaa cggatcgagc cgcacgccgt agctgctcga
     8221 ctgggccgcc gcgctggtcg ggcggaagaa gggcaggccg cgcgcggccg tggccagcga
     8281 gcgcatgtcg gacatattgg acgaggcgcg ctggatccgc tgggcgaagc tctcgtcgac
     8341 gtcaagcacc gcggccaggg cccgcgggtc gcgagcctcg atcagcgggc cgccgaggcc
     8401 gccgccgcgg gcggcgtagc tgtcagggtt aagtccggcg aggcggaagg cgaggcgaag
     8461 ccgttcggcg cgactgaggg cgtagctctc ggcggcgctg atcatccgct cctggtccat
     8521 cagggtgtac tgcacccgct gaaccggcga gccggcgttg agccgcggct tggcgggagc
     8581 cagcgcggcc atggcgccgg gcgcgccctt gaattcggtc agcagcaggg cgagggcggc
     8641 gtgccgcttc tcgaccgaac tggccagctc tccgagcgag ccgtcggtct gcgacagctg
     8701 ctgcatggcg ctgttcaggc gcgcctggcg atcggcggtg acccgttccc agtaggcgtt
     8761 gcggcgggcc agttcctggt cggtggcgct gaccgacagg gcgctgacga gcatcgccgc
     8821 ggtgcagacg cccatccaca gggctgccgc gccgatgagc ccggcgccgg cgatctgctt
     8881 gccggtggac aggacgaacc cgcgcatctc gccgcccgaa cggatgtaga gatgccgttc
     8941 cggaaacagc tcctccaagg cccgccgaat tctgcgcaag cgtgctgtcg ccatagcccc
     9001 cgaacccgag ttttcggttt ccggcgatat gcggccaact ggggggaacg cgcaagaccc
     9061 ttcgcgcgtg aggtgtgcgc gaagctggga ttctgacctt gcgagggcag gattaaccat
     9121 gactgcccat tagaccagtt cctgccctca actttgctcg ctgggcggga acatttggag
     9181 gcgggcgcgg cgttttcgca acacttggag ttaccgctgg gtaacgggtc gtttcgggcc
     9241 ggggtgcggc cgaacgtcgc cggaggagcg cctagtgggc ccgggcggcg gccaggacct
     9301 cctcggcgtg gcccttgacc ttgaccttgc gccagacctt gcggatcacg ccctcgccgt
     9361 cgatcagcca ggtcgagcgg tcgatgccca tgtgctcccg cccgtagagg ctcttctgaa
     9421 cccaggcgcc gtagcgctcg atgacgtcac tgtcggcgtc ggagcccagt tccaccgtca
     9481 ggccatgctt cgcgcgaaac ttgccgtgtt tggcggccgt gtccttcgac accccgacga
     9541 gcacggcgcc ggccgcgtcg aactcggcct tgaggcccga gaaggcgatg ttttctgtcg
     9601 tgcagccggg cgtgtcgtcc ttgggataga agaacagcac gaccgtcttg cccttgaggg
     9661 ccgacagcga gacgcggccc gtgtcggtcg gcaggtcgaa gtcgggggcg cggtcgccaa
     9721 tggtcaggtc agtcacgaaa tgccctccat gaatcccgct cttccccgcg aaggcgggga
     9781 cccaggcttt ttttgaaacc gcggctcacc ttcaaggttg gcgcgccgca tggtcggaac
     9841 cgtgagccgg agctctgaaa aaacctgggt ccccgccttc gcggggatga gcggagtatg
     9901 gtggatcaga cggggcgaac ccagacgatc ttgttcttgc ccttctggat cttgaacagg
     9961 ccgtcatcgc ccgtgccatc ggtgcggttg tagtgtgcct tcggatttgt gatccgcaca
    10021 tcattgatca ggattgtccc ctgctcgatg agattgcgtg cttccttgac ggatccggca
    10081 agtccggcgc ccttcacaat ggcctccgcg ctacgttggg tgagatcgcc gttctcaccg
    10141 gcccagagaa agtccaccga ggtcgcggca gcagccgtgt tgcccagttc gaaggcctct
    10201 tcagccttgg cccgcgcggc cctggcggcg tccgctccgt gcaacatccg cgtcgcctcg
    10261 tccgccagca ccttcttggc gtcattgatc gccgcgccct ccagcgcctc cagccgcgcc
    10321 acctcgtcca gcggcaggtc ggtgaacagg cgcaggaagc ggccgacgtc ggcgtcctcg
    10381 gcgttgcgcc agaactgcca gtagtcgtag gggctgcgca tgtcggcgtt gagccagacc
    10441 gcgccctggg cggtcttgcc catcttggcg cccgaggccg tcgacagcag cggggtggtc
    10501 aggccgaagg ccggcttctg gtcgacgcgg cggatcagct ccaccccgtt gaggatgttg
    10561 ccccactggt cggacccgcc catctgcagc ctgcagttcc gcgtgcgcga cagctcgagg
    10621 aagtccacgg actgcatcag catgtagttg aactcgagga aggtcatcgg ctgctcgcgc
    10681 tcgagccgct gcttgaccga gtcgaacgcc agcatgcggt tgatcgtgaa gtgcacgccg
    10741 aactcgcgca ggaactcgac atagccgaac ttcgagagcc actcgtcgtt gtcgaccatg
    10801 accgcgtcgg tcgggccgtc gccgaaggtc aggaacttct cgaacaccgt cttgatgctg
    10861 gcgatgttgg cccggatgtc ggcctcgctg agcagcttgc gcgactcgtc cttgccggtc
    10921 gggtcgccga ccttggtcgt gcccccgccc atcagcacga tcggcctgtg cccggcctgc
    10981 tgcagccggc gcagcatcat gatctggatc aggctgccca cgtgcaggct gggcgcggtg
    11041 gcgtcaaagc cgatgtaggc ggtgaccacg ccctgggtcg ccgccgcgtc cagctcctcc
    11101 ggatgggtga tctggtggat gtagccgcgg tcctgcagcg tgcgcaggaa gtcggacttg
    11161 aaggcggcgt cggcggtcat cggggcaggg tctccttgcg cggacggctc tagcacgtcg
    11221 ggcggggagg ggttcaaggt catcagcatg gaaaggtctc ctcggaatga ggccggagac
    11281 aatctgctgg aagggtcaga gcatgccgcc ggcggtgtgg cggcatgcga tcgcaacgcc
    11341 gctcgtcagg acgagcgggc gtaataaagg ctgcaaaggg tgaaccgggc gatcatgggt
    11401 tgctgaatgc ctgcggtgtt gaccagcgtc aagcaggggc tatcgaaggg ggatgctgct
    11461 ggcgctgacc tgtcaccccg acaccccttg cgacgcggtc tcgcggatcg aggtcaatct
    11521 ggcgcgaggg ggcggggcga gcctggcgct gcgctatgtg acgagcggcg atgtcagccg
    11581 catcagcctg cccgggcgcg ctgaacgcgg ccgcgccgac gggctctgga agaccacctg
    11641 tttcgaagcc ttcgtgcaga tccccggagg ctatgccgag atcaaccttt caccgtcaga
    11701 ccaatgggcc gcctacgggt tcgccggcta tcgcgacggc gccatggcgc agctgccggg
    11761 cgccgccctg cgacacatga cccagcgggc gggcgagggg ctttacgagc tggtcgccac
    11821 gcttgatctg gccggactcg atctgcccgt ggaccagccc tggcggctgg gcctttcagc
    11881 ggtcatcgag gaggccagcg gccgcaagtc ctactgggcg ctggcgcatg gccccgacaa
    11941 gcccgacttt catcaccccg actcgttcgc ctgcgtcctg gagccctcat gaacttcggc
    12001 atcgaccgcc tgctcgccga tcccgccctg cgcgcgccac tggccggcaa gcgggtggcg
    12061 ctgctcgcgc atccggcgtc ggtgacggcg gatctgaccc attccatcga cgccttggcg
    12121 gcctgccccg acatccagat caccgccgcc ttcgggccgc agcacggcat gcgcggcgac
    12181 ctgcaggaca acatgatgga gtcgccggac ttcaccgatc cggtctacgg cttccccgta
    12241 ttcagtctct atggcgagac gcgccgcccg accggccagg ccatgggcac gttcgacgtg
    12301 atcctggtcg acctgcagga cctcggctgc cgcatctaca ccttcatcac cacccttctg
    12361 tacgtgctcg aggccgccgc cgagcacggc aaggaagtct gggtgctcga ccggcccaac
    12421 cccgccggcc ggcccatcga agggctgacc ctgttgccgg gctgggagag cttcgtcggc
    12481 gccgggccgc tgccgatgcg ccatggcctg acgctcggcg agctgggcca gtggttcgtg
    12541 cgcagccgca gcctggatgt cgtctatcgc gtgatcgcga tggagggcta cgaccccgct
    12601 gccggacccg gctacggctg gccgctgggc gagcgcagct ggatcaaccc cagccccaac
    12661 gcacccaacc tctcgatggc ccgcgcctac gccggcacgg tgatgctgga ggggacccac
    12721 ctgtccgaag gccgcggcac gacccggccg ctggagctgt ttggcgcgcc ggacatcgac
    12781 gcccgcaagg tcatcgcgga gatgaacgcc ctggccccgc agtggctgaa cggctgccgc
    12841 ccgcgcgaca tctggttcca gccgaccttc cacaagcatg tcggccagct gtgcaacggc
    12901 gtgcagatcc acgtcgacga tccggcctac gaccacgccg cgttccggcc ctggcgactg
    12961 caggcgctgg ccttcaaggc catccgccgg ctgtatccgg actatcggct gtggcgggac
    13021 ttcccgtacg agtacgtgtt cgacaaactg gccatcgacg tgatcaacgg cggccccggt
    13081 ctgcgcgagt gggtcgatga tcccgccgcc atgccggacg acctcgacgc cctggccacg
    13141 cccgacgagc aggcgtggga agcggcccgc cgccccttcc tgctgtactg atcgccatga
    13201 cgatgcgcat cgccctgctg cgcggcgtca atgtcggcgg cgcgaagaag atcgccatgg
    13261 ccgagctgaa ggccatggtc gaggccttgg ggttcacgga tgtgaagacc ctgctgcaga
    13321 gcggcaatgt ggtgttcgag gcgggcgagc ggagcgacgc cgaactggaa gcgctgctgg
    13381 agactgaggc cgaacggcgt ctgggcctgg tcacccgctt catcgtgcgg ggccccaagg
    13441 cctggcggat gatgatcgac gccaatccga tgccgacagc agccgaacgc gagccgtcgc
    13501 ggtttctggt gaatgtaacg cgcgagccgg tgacgccgga gatgctggct atgatccggg
    13561 cggcggcgac gccgggcgag cggatcgagg ccggagatcg ctgcgtctac gccttcttcg
    13621 gcgacggcgt ggccgactcg aaagccgcgc tggtgttcgg gaagaaggcg ctggggctca
    13681 catccaccgg ccgcaactgg aacacggtgc agaagatcgc ggcgctgatc ggggcgtgaa
    13741 gaggggacat gcactgaagt gcatgtgccg atttcagaag ggtcgagtca ccagcaacgc
    13801 gacggcgagc gcggccagca ggccggccgc cgcgagcacg acttccgcgc ggcgggccag
    13861 gaggccgctc gtctctttca taaccttccg ctggaacagg ccccaggcgc cggccttggc
    13921 ggccagcaag gccgccaccg cgcaaaggac gcctacggtg accgaggccg gcatggggtc
    13981 aggccttttc cgccttcgcc cgctcgatcc cttccagcac catcctgcgc gcctcggccg
    14041 cgtcgcccca gcccttgacc ttgacccatt tgccgggctc gaggtccttg tagtgggtga
    14101 agaagtgctc gatctggtcc agcgtgatcc gtggcaggtc ggtgtagtcc ttcaccgcgt
    14161 cgtagcgcgc ggtcaggtgc gaggatggca ccgcgatcag cttctcgtcg atgccggcct
    14221 ggtcctccat cagcagcacc ccgacgatgc ggcagctcat taccgcgccg ggcgcgatgg
    14281 cccgggtgtt gaccacgatc acgtcgaccg ggtcgccgtc gcccgacagg gtgtggggga
    14341 tgaagccgta gttccccgga tagcgcatgg ccgtgtagag gaaccggtcg acgaccagca
    14401 ccccggcgtc cttgtcgagc tcgtacttga tcggctcgcc gccgatcggc acctcgatga
    14461 tgacgttgac gtcctcgggc ggattgcggc cgatggcgat ggcgtcgaga ttcatgggtt
    14521 cgctactcgg aaagtcaggc cggttcggct tccaggcgct tgtcgaggta ggccccgacg
    14581 cgcttctcga cttccggcag atgctcggcc cagaagtggg tcgcgccctc gaccagttcg
    14641 tagtcgatga cgatgccctt ctgggtgcgc agcttgctga ccacccgctc gacctcgacc
    14701 ggcggcacca cggtgtcggc ctggccgtgc aggatcaggc ccgaggccgg gcagggggcg
    14761 aggaagctga aatcgtacat gttggtcggc ggggagacgc tgatgaagcc gtcggtctcc
    14821 ggccggcgca tcagcagctg catgccgatg taggcgccga agctgtagcc ggcgacccag
    14881 aactggctgg cctgcgggtt ggtcgcctgc agccagtcga gcgccgtggc cgcgtcggcc
    14941 agttcgccga tgccggcgtc gaactcgccc tggctgcggc ccacgccgcg gaagttgaac
    15001 cgcagcaccg agaagccgcg cttcatgaac aggtgataga gctgcacggc caccggattg
    15061 ttcatgtggc cgccggcgcg cgggtgggga tgcaggatca gggcgatcgg cgcgttctcg
    15121 cgcttgcccg gcgaatatcg accttcgatc cggccggccg cgccggtcag caccacttca
    15181 ggcatgcgct tttcccgtct tccagggccg cggaatactt gaccacagcg cttggttttc
    15241 ctagatcacc gacgtccgct caggccttcg cgaaggcccc tgcgggcgag gcgcggcttt
    15301 agcacaggcc gcgcgccgga tgccaaggaa tctcgccatg cgcctgtcca caaagggccg
    15361 ctacgccgtg atggccatga ccgacctcgc cggccggcag ggggccgacg actcgcttcg
    15421 ggccgtgtcg ctggccgaga tcgccgcccg ccagcagatt tccctgtcct atctcgagca
    15481 gctgttcgcc cgcctgcgcc gcaagggcct ggtcaggagc gtgcgcggtc cgggcggcgg
    15541 ctaccgcctg gcccagagcg ccagcgagac aaacatcgcc gacatcgtgc tggcggtcga
    15601 cgagcccctg cgcgcgaccc gctgcggcaa gccggtcggg cgtggctgca tggtcggcgg
    15661 cgagcgctgc ctgacccatg acctgtggga cgagatgggc cgacagctgc aggcctatct
    15721 ggcgtcggtg accctggccg acgtgctcag cggcaagctg cgcgtggccg cctcggaggc
    15781 cgcgtgaccc tctatctcga ctacaacgcc accgctcccg tccgccccga ggcgaaggcc
    15841 gccgtgatgc gcgcgctgga gatcggcggc aacccctcgt cggtgcacgc cgccggccgg
    15901 gccgcgcggt cgctgatcga ggccgcgcgg gcccaggtgg cggacctcat tgcggtcaaa
    15961 ccgggttcga tcaccttcac cagcggcggc accgagtcca acgccctggc catcgagagc
    16021 gccgtggccg cgggcgtcga ccgcatcctg ctgggcgcga ccgaacacgg cgcggtcagc
    16081 gagaatgtca aggcgaccgg ggttccggtc gaggtctggc ccgtggacgc ggatggcgtc
    16141 gccgacctgg cctggctggc cgacgctctc ggcaagggcg gcaggccgct ggtctgcctg
    16201 atgctggcca acaacgagac gggggtgatc cagccggtcg ccgaggcgtc ggcgctggtc
    16261 cgcgaggccg gcggctggct gcacgtcgac gcggtccagg cggccggcaa gatcgccatc
    16321 gatttcaacg ccctcggcgc ggacaccctg gcgctggcgg cgcacaaact gggcggaccg
    16381 ttcggggtcg gcgcgctcgc ggccgggccg cgcgcg
//
LOCUS       DAORNX010000010        15821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2837_length_15821_cov_8.599074, whole genome shotgun sequence.
ACCESSION   DAORNX010000010 DAORNX010000000
VERSION     DAORNX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_15821_cov_8.599074"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLO65_00890"
     CDS             <1..377
                     /locus_tag="PLO65_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRD26835.1"
                     /translation="LPARAVAQKAKSARPPETIEPGRHKRIVRGHEPLEARIDLHGMT
                     HDTARAALQAFLLRVWDEGHREVLVITGKGTRGDGVLRRFTPDWLSAPPLRDIVAGVS
                     EAHRRHGGEGALYIALKRKPRA"
     gene            448..1956
                     /locus_tag="PLO65_00895"
     CDS             448..1956
                     /locus_tag="PLO65_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD26836.1"
                     /translation="MTERVLVIGAGMAGLFTALALGGEGRQIDILERDPPTPTGGADE
                     AFHDWNRRGVGHLRHSHAFLARLRSLVRDHHPALLEDLRAAGCQEMKFADGLPAASRD
                     RYRPEPGDEILTVITSRRTTLELVIRRYVERMPGVTLHSSVNVTGLVGSLDAEGRFVC
                     EGLRLKDGEAEREERADVVIDAAGRTSQGFDWLAEQGLSWDEESEDAGILYYTRHWRL
                     LPGQSPPERGPVPGAGDMGFIKYGVFPADNGCFSITLSVPEIEETLRLAVMRPEIFDR
                     ICQLLPGVAPWIAADRSEPVSKVYGMGDLKSRWRSTVRDGKPIALNLFFAGDGLARTN
                     PLYGRGCSFAAIAAYLLRDVLAGDRDPVARALAYQAAVDSDLRPYFRDMRSQDRQAIR
                     RARNTLDPSYRPRFKARLMKSFAEDAIAPALRSDPALLRAFMRGFHMLEPSSTWLKRP
                     STFAKVLTAWAKPRALKQKYYPPKLGPDRAEMFAALELSATADAQRLGLVAA"
     gene            complement(1936..2274)
                     /gene="hisE"
                     /locus_tag="PLO65_00900"
     CDS             complement(1936..2274)
                     /gene="hisE"
                     /locus_tag="PLO65_00900"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRD26837.1"
                     /translation="MSRLSEVIARLEAVIESRKGGDPATSWTARLLADPALAGRKLIE
                     EAGEAVEAAESGDRDHLAAEAADVIYHLLALLAAAGASPDAVAEKLEAREGRSGIAEK
                     ASRGSGGDQT"
     gene            complement(2271..3047)
                     /gene="hisF"
                     /locus_tag="PLO65_00905"
     CDS             complement(2271..3047)
                     /gene="hisF"
                     /locus_tag="PLO65_00905"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRD26838.1"
                     /translation="MLKVRVIPCLDVKDGRVVKGVNFVALRDAGDPVEQAAAYDAAGA
                     DELMFLDITASHEGRGAILDVIARTADVCFMPVSVGGGVRTVEDARRLLRAGADKVSI
                     NTAAVEDPDLVARMADAFGSQCVVVAVDAKGRGDGGWNIFTYGGRNDTGVDAVEYAAR
                     AVEKGAGEILLTSMDRDGARTGYDLPMLRAITGAVSVPVIASGGAGNTRHLVEAVTLG
                     GADAVLAASIFHFGEVSIPDAKRAMAAAGIPVRGIREIAA"
     gene            complement(3047..3769)
                     /gene="hisA"
                     /locus_tag="PLO65_00910"
     CDS             complement(3047..3769)
                     /gene="hisA"
                     /locus_tag="PLO65_00910"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRD26839.1"
                     /translation="MILYPAIDLKDGRCVRLLHGDLNKETVFNNAPADQAALFQKQGF
                     SWLHVVDLNGAVEGRPVNGEAVAGILGAVSIPVQLGGGVRTMASIEGWIEGGVSRVIL
                     GTVAVKDPALVKAAARAWPEQIAVAIDARDGKVATEGWVGDSDLGVIELARRFEDVGV
                     AALIVTDIGRDGAMTGVNIDQVGEVADAVAIPVIASGGIASVRDIELLKARQGAAIAG
                     AVLGRSLYEGAVDPTQALKIAG"
     gene            complement(3766..4410)
                     /gene="hisH"
                     /locus_tag="PLO65_00915"
     CDS             complement(3766..4410)
                     /gene="hisH"
                     /locus_tag="PLO65_00915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRD26840.1"
                     /translation="MQSVALIDYGSGNLRSAEKALRRAAEGLATPVDIIVTADPDVVS
                     RADRLVLPGVGAFAACMGALSARDGLIAAMTETAIVRGCPFLGICVGMQLLADRGLEF
                     GETPGLGWIPGDVARLHPADPAAKIPHMGWNALEDLADHPLLRGLEAGAEMYFTHSFA
                     FAPADPSHIIARVDHGGRFAAAVARETIMGVQFHPEKSQTQGLRLLANFLETPA"
     gene            complement(4420..5010)
                     /gene="hisB"
                     /locus_tag="PLO65_00920"
     CDS             complement(4420..5010)
                     /gene="hisB"
                     /locus_tag="PLO65_00920"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRD26841.1"
                     /translation="MARTAEVTRETKETQIRVRIDLDGTGEAKVSTGIGFFDHMLDSF
                     ARHGGFDLEVQTTGDLHIDMHHTVEDTGIVLGQAVNRALDGFKGIRRFGSAYIPMDET
                     LTRCALDLSNRPYLIWKVAFRTPLVGTMDTELFKEFHHAFAMNAGACVHLETLYGDNS
                     HHIAESGFKALARALRTAVELDPKTHGRAPSTKGSL"
     gene            complement(5010..6002)
                     /locus_tag="PLO65_00925"
     CDS             complement(5010..6002)
                     /locus_tag="PLO65_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRD26842.1"
                     /translation="MPELYDVAAIGNAIVDVIAPCDDAFLDSQGLVKNSMQLIDEGQA
                     EELYGHMAPGIETSGGSAGNTVAGVASFGGRAAFLGKVADDQLGEVYEHDMKAIGVAF
                     DSRPLVGGPRTARSLINVTPEGHRTMCTFLGASTELGPDDIDPRMIESARIIFLEGYL
                     FDPPEARRAFAKAAGLSRAAGRLMSITLSDSFVVERHRDVLLAFIETECDIVLANESE
                     VKAMFQTDDFDAAAQALVDRTRICAITRGETGSVVFAGGASHIIPAYPVEKVVDTTGA
                     GDQYAAGFLLGLARGRSMDVCGRLGALAAAEVISHYGPRPQVSLETLAKAEGLF"
     gene            6152..6439
                     /locus_tag="PLO65_00930"
     CDS             6152..6439
                     /locus_tag="PLO65_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD26843.1"
                     /translation="MAAYMIFIRDKTTDAAELATYSGLAGASMGGHAVTPHVVYGPHE
                     VLEGPAVEGVVVVSFPTLEEAKAWYHSPAYSAAREHRFKGAEYRVVLVQGL"
     gene            6471..7322
                     /locus_tag="PLO65_00935"
     CDS             6471..7322
                     /locus_tag="PLO65_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRD26844.1"
                     /translation="MARIALLTPAAHNPYVRVWGPAFDRLMAALSGGGLQVIAMEWPK
                     AAQADVDAVLPLLAWGYHQDAADWLAMLDALEAQGVKALNPAAVLRWNTSKTYLADLE
                     AAGVPVVPTVFVDRADDAAVAKARAGLGADTVVVKPQVSGGSHETVKLAPGEALVGGP
                     SGAAMIQPFLPSVSGEGEISLLYFDGVFSHAIGKVAKAGDFRVQPQFGSTIGPIEPPP
                     GALDVAGKVLAAAPGPLTYCRIDLIRHPDGTLRLMELEAIEPDLFLEHAPEAGAVFSA
                     AVAKALR"
     gene            complement(7356..8030)
                     /gene="nth"
                     /locus_tag="PLO65_00940"
     CDS             complement(7356..8030)
                     /gene="nth"
                     /locus_tag="PLO65_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRD26845.1"
                     /translation="MNKVAPKRRLSPKEKARVAALFDRFLTLSDDPRTELKFQDPFTL
                     VVAVALSAQATDVQVNKATAKLFPVAHTPQQMLALGEAGLTGYINSIGLFRNKAKNVI
                     ALSRIILEKHGGKTPLNRADLEALPGVGRKTASVVLNELGIEPAIAVDTHVFRVAHRL
                     GLSTGKTPDRVEQDLMAIVPEPFLTRAHHWLILHGRYVCLARKPKCEECQVRDLCPSR
                     ELFLGG"
     gene            8094..8573
                     /locus_tag="PLO65_00945"
     CDS             8094..8573
                     /locus_tag="PLO65_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRD26846.1"
                     /translation="MAAALYMDAEIRPNRSMPATGLKVLMGVMIVASVMLSIVMFVMG
                     AWFAPAFLGLDVLGVWLAFRASYRAQERRERVRVTAETVTVLHELDGEARTVWTSPTA
                     FTGVDVDWPGEHEARVRLRLSARRCTVGGALSPEERVGLGEALREAIRRARAERYAS"
     gene            8623..9519
                     /locus_tag="PLO65_00950"
     CDS             8623..9519
                     /locus_tag="PLO65_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRD26847.1"
                     /translation="MAYDMTPTRSTLAPASGVIIQLEERARDYDRMARALAWLADRWR
                     DHPSLDEAADAVGLSPFHFQRVFTRWAGVSPRAFVASIAHAEARALLEEGASVLDAAL
                     DTGLSGPSRLHDLFIAQEAVTPGETRRRGEGLILRWGLAPTPFGTGLFAVAPRGLAGL
                     GFVDEAGEDAAFADMHRRFPAADWVRDDAAARETAIKAFAEGEGPLPIVLIGSHFHIQ
                     VWKALLRIPAGATATYGDVARWAGKPKAFQATGAAIGANPVSLLIPCHRVIARDGRLT
                     GYHWGLGRKAAMLGMEAVAAAG"
     gene            complement(9539..10402)
                     /locus_tag="PLO65_00955"
     CDS             complement(9539..10402)
                     /locus_tag="PLO65_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRD26848.1"
                     /translation="MRLALTLAFAAVVALPARAAVAPVTPEPVVAAERAFAADGYVMG
                     IKKSFLAHMADDAILMTPDPVNARETFLASPDDRPDAPKLEWWPTWAGISASGDLGFT
                     TGPYSVGGQRRGHYFTVWKKQADGRWKWVFDGGVGSDPAASPGPGAAPGYLAMPKVPG
                     LYPEGAFAKVRAAEAALATEAGVDSKAAYLKVLACDGRIQSSPMAPATGCATFGPELD
                     WRAARIAFAPLGGDISVAGDMAWTYGTAGWDKDGKPVKAHYVRVWQRRPEGWRIVFDE
                     LLIPRTPAAAE"
     gene            10516..11253
                     /locus_tag="PLO65_00960"
     CDS             10516..11253
                     /locus_tag="PLO65_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRD26849.1"
                     /translation="MAFSPAEVERYARHLVLREIGGPGQQRLKATSVLMVGAGGLGAP
                     ATLYLAAAGVGRIGVADPDTVALSNLQRQVLYTAADVGLAKVTVAAGRLTALNSDIVV
                     EPHALAVTPDNAATLVAGYDLVLDGTDDFATRFAVSDACVSAGKTLVSGAIARWTGQV
                     GVFPGRPCYRCLVPGIPPDADTCARVGVVGALGGVIGSMMALEAIKVITGAGEPLSGR
                     LLIYDALAGETRTVRIGSDPGCPACHA"
     gene            11324..12319
                     /locus_tag="PLO65_00965"
     CDS             11324..12319
                     /locus_tag="PLO65_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD26850.1"
                     /translation="MKTLGRILIGVVVIIAVVLTGGWLVLRRPDIPWPTLQAKYANSA
                     SRYMDLPGGLKVHYRDQGRRDGPVIVLVHGFAASLHTWEPWVARLGSEYRIITLDLPG
                     FGLTSAPDGYTVARSDFVDVVDRLTAKLGVTKFVLAGNSMGGGVAWNYALKHPDKLEG
                     LVLVDAAGWPQQRDDGRDGPFIFKVLRNPVGRFLVRDLDVSGMTRAGLRDAFAPTPEM
                     VDDAMVARYVEMSRAPGHRDIVLTMMGGYDPADAASKEKLAKIVVPTLVMHGDTDRLI
                     PVSHAALFGQAIPGSTVIIYPRVGHVPMEQIADKSAADLDAWLRARVWPRPPVNR"
     gene            complement(12309..14321)
                     /locus_tag="PLO65_00970"
     CDS             complement(12309..14321)
                     /locus_tag="PLO65_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD26851.1"
                     /translation="MITRRRLVALAAAGPVAFAGVPALAAEVASGAAPPTLEDLTREA
                     VVLDAALSPTGDRIALLREQREGDRRLSYVLLAKVADMRAPPIQAVIGDYDVHRVQWA
                     SNDRLLVWFSFDKTADGRKTGIRAGARLYERSVHRLMAIGADGGNPVVLLGQDRSAQS
                     YIWNVSHVVDPMVGDDDHILMQVWDPDLYANTLQKVNIHTGKGELHERGSERTRFWYT
                     QNGVAVLRVDQSELGRYTRLSARPPGARDWQFVRKIRSNDFSQFDDFEAVGATDEAGV
                     LLVATMAPGDDARTIRRFDIRTLALGEVVASHAGRDMEGVFFDKGLNPVAAAYTENRT
                     QYEFLDAALAPHYRAVQNALGADANVLLHDIDAGRRRFIVHASGPRESGAFYLYDRET
                     RSLDLLGRQKPWLPAERLAPMEVLTVRSRDGVELAAYLTRPLATGPRPMVVMPHGGPH
                     ARDSFAFDLFAQAFASKGWLVLQPNFRGSSGYGRLFAEAGHRRWADRMQEDVEDCVAH
                     VVASGAVAPGRIAICGASYGGYAALMGGIRKPDLYRRIVSIAGVTDLPDLLSYEREDG
                     ADSPSYLYFVKTVGDPATDREALKRGSPNERAAELAAPVLLIHGDVDGVVPIRQSRAM
                     AASLKRENRPARLIELRNAGHGGWEPDVLSQILLETTAFIDEGFSG"
     gene            complement(14386..15372)
                     /locus_tag="PLO65_00975"
     CDS             complement(14386..15372)
                     /locus_tag="PLO65_00975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRD26852.1"
                     /translation="MAAKKPKVVVTRKLPDPVETRMRELFDTELNVDDRPMTPDELVE
                     AMGRADVLVPTITDRLDSRLLSRAGERLKLIANFGAGVDNIDVATANQRGVIVTNTPG
                     VLTEDTADLTMSLIMASTRRIVEGANVVQSGGFQGWSPTWMLGRRLWGKRLGIIGMGR
                     IGQALARRGKAFGLSIHYHNRKPVSPRVAEELEATYWESLDQMLARMDIVSINCPHTP
                     ATYHLLSARRLKLLQPTAVIVNTARGEVIDEGALANMLARGEIAGAGLDVYEHEPAIN
                     PKLLKLPNVVLLPHMGSATVEGRIDMGEKVIVNIKTFMDGHRPPDRVIPAML"
     gene            15482..>15821
                     /locus_tag="PLO65_00980"
     CDS             15482..>15821
                     /locus_tag="PLO65_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRD26853.1"
                     /translation="MPVKLALIALAVTLAGCNGRGAQVCDTPSTLPVPRFVSLKFGEV
                     YARKGPGEDHRILWVWRSKGMPLEVVAETSDWRRVRGPDGALAWAHKRGLDGRLTVMR
                     VRPGPLPMRAA"
BASE COUNT         2285 a         5581 c         5493 g         2462 t
ORIGIN      
        1 cgctgccggc gagggcggtt gcccagaagg ccaaatcggc ccggccgccc gaaaccatcg
       61 agccgggacg ccacaagcgc atcgtgcgcg gccacgagcc gctcgaggcc cgcatcgacc
      121 tgcacggcat gacccacgat accgcccgcg ccgccctgca ggcctttctc ctgcgggtct
      181 gggacgaggg ccaccgcgaa gtcctcgtca tcaccggcaa gggcacgcgg ggcgacgggg
      241 tgctgcggcg gttcacgccc gactggctgt cggcgccgcc cctgcgcgac atcgtcgcgg
      301 gggtgtccga ggctcaccgc cgccatggcg gcgaaggggc gctgtacatc gccctgaagc
      361 gaaagccccg ggcctgacct cgcgtcatac tggtcggcca ccggcggggt tgtcatagtc
      421 tgccgcgaga cgaccgagga ctaccccatg actgaacgcg tcctggtgat cggcgccggc
      481 atggccggcc tgttcaccgc cctggccctg ggcggcgagg gtcgccagat cgacatcctg
      541 gaacgggacc cgccgacccc gaccggcggc gccgacgagg cgttccatga ctggaaccgc
      601 cgcggcgttg gccatctgcg gcatagccac gcctttctgg cccggctgcg cagcctggtg
      661 cgcgaccatc atccggcgct tctcgaggac ctgcgcgcgg ccggctgcca ggagatgaag
      721 ttcgccgacg gcctgccggc cgccagccgc gaccgctacc gccctgaacc gggcgacgag
      781 atcctcacgg tcatcaccag ccgccgcacg acgctggaac tggtgatccg ccgctatgtc
      841 gagcgcatgc ccggcgtgac cctgcacagc agcgtcaacg tcacgggcct ggtcggcagt
      901 ctcgacgcgg aaggccgctt cgtctgcgaa ggcctgaggc tgaaggacgg cgaggccgag
      961 cgcgaggagc gcgctgacgt cgtcatcgac gcggccggcc gcacctcgca gggcttcgac
     1021 tggctggccg aacagggtct gagctgggac gaggagagcg aggacgcggg cattctctac
     1081 tacacccgcc actggcggct gctgcccggc cagtccccgc ccgaacgcgg cccggtcccg
     1141 ggagccggcg acatgggctt catcaagtac ggcgtgtttc ccgccgacaa cggctgtttc
     1201 tcgatcaccc tgtctgtgcc ggaaatcgag gagaccctgc gcctggcggt catgcggccg
     1261 gaaatcttcg atcgcatctg ccagctgctg cccggcgtcg ccccctggat cgccgcagac
     1321 cggtccgagc cggtcagcaa ggtctacggc atgggtgacc tgaagagccg ctggcgctcg
     1381 accgtcaggg acggcaagcc gatcgcgctg aacctgttct tcgccggcga cggcctggcc
     1441 cggacgaacc cgctgtatgg tcgcggctgt tcgttcgccg ccatcgccgc ctacctgctg
     1501 agggacgttc tggcgggtga ccgtgatccc gtggcccggg cgctggccta ccaggccgcc
     1561 gtcgacagcg acctgcgacc ctacttccgc gacatgcgca gccaggaccg gcaggcgatc
     1621 cgccgggccc ggaacaccct ggacccgtcc taccgcccgc ggttcaaggc gcggctgatg
     1681 aagagcttcg ccgaggacgc catcgctccg gccctgcgct ccgatccggc cctgttgcgg
     1741 gccttcatgc gtggcttcca catgcttgag ccgtcctcga cctggctcaa gcggccatcg
     1801 accttcgcca aggtcctgac cgcctgggcc aagcccaggg cgctgaagca gaaatactac
     1861 ccgcccaagc tcggtcccga ccgggcggag atgttcgcgg ccctggagct ttccgccaca
     1921 gccgacgcgc agaggctagg tctggtcgcc gcctgagccg cgcgaggcct tttcggcaat
     1981 gccggaccgg ccttcccgcg cttccagctt ttccgcgacg gcgtcgggcg aggcgcccgc
     2041 cgccgccagc agcgccagga ggtggtagat cacatcggcg gcctcggccg ccagatggtc
     2101 acggtcgccg gattccgccg cctcgaccgc ctcgccggct tcctcgatca gttttcttcc
     2161 agccagcgcc ggatcggcca gcagcctggc cgtccaggat gtcgcgggat cgccgccctt
     2221 gcggctttcg atcacggctt ccagccgcgc gatgacctcg gacagccggc tcatgcggcg
     2281 atctcccgga ttccgcggac gggaatgccc gccgccgcca tggcccgctt ggcgtccggg
     2341 atcgagacct cgccgaagtg gaagatcgag gccgccagca cggcgtcggc cccccccagc
     2401 gtcaccgcct cgaccagatg ccgggtgttg cccgccccgc cgctggcgat gaccggaacg
     2461 ctgaccgcgc cggtgatggc ccggagcatc ggcagatcgt agccggtcct ggctccgtcg
     2521 cggtccatcg acgtcagcag aatctcgccc gcgcccttct ccaccgcccg ggccgcgtac
     2581 tcgaccgcat cgacgccggt gtcgttgcga ccgccatagg tgaagatgtt ccagcccccg
     2641 tcaccccggc ccttggcgtc gaccgccacg acgacacact gcgagccgaa ggcgtcggcc
     2701 atgcgcgcga cgaggtcggg gtcctccacg gcggcggtgt tgatcgagac cttgtcggcc
     2761 ccggcgcgca gcaggcgcct ggcgtcctcg acggtgcgaa cgccgccgcc gaccgacacc
     2821 ggcatgaagc agacgtcggc cgtgcgggcg atgacatcga ggatcgcccc ccgaccctcg
     2881 tggctggccg tgatgtcgag gaacatcagc tcgtccgccc cggccgcgtc ataggcggct
     2941 gcctgctcga ccgggtcgcc ggcgtcgcgc agggcgacga agttgacgcc cttgaccacg
     3001 cggccgtcct tgacgtcgag acaggggatg acgcggacct tgagcatcag ccggcgatct
     3061 tcaacgcctg ggtcgggtcg acagcgccct cgtagagaga ccggcccagc acggcgcccg
     3121 cgatggccgc gccctgccgc gccttgagca gctcgatgtc gcgcaccgag gcgatgccgc
     3181 ccgaggcgat caccggaatg gcgaccgcgt cggcgacttc cccgacctgg tcgatattga
     3241 ccccggtcat ggccccgtcg cggccgatgt cggtgacgat cagggcggcc acgcccacat
     3301 cctcgaaacg gcgcgccagt tcgattacgc cgaggtcgga gtcgccgacc cagccctcgg
     3361 tggcgacctt gccgtcgcgc gcgtcgatgg ccacggcgat ctgctcgggc caggccctgg
     3421 cggcggcctt gaccagcgcc ggatccttga ccgccacggt gcccaggatc acccggctga
     3481 cgccgccctc gatccagcct tcgatcgagg ccatggtccg caccccgccg cccagctgga
     3541 ccgggatcga gaccgcgccc aggatgccgg ccaccgcctc gccgttgacc ggccgcccct
     3601 cgaccgcccc gttcaggtcg acgacatgca gccaggaaaa gccctgcttc tggaacaggg
     3661 cggcctggtc ggcgggcgcg ttgttgaaca cggtttcctt gttcaggtcg ccatgcagca
     3721 gacgcacgca gcgcccgtcc ttcaggtcga tggccggata gaggatcacg cgggcgtctc
     3781 caggaagttc gccagcagcc tcagcccctg ggtctgggat ttctcggggt ggaactgcac
     3841 ccccatgatg gtttcacgcg ccaccgccgc ggcgaaccgc ccgccgtggt cgacacgggc
     3901 gatgatgtgc gacggatcgg ccggggcgaa ggcgaagctg tgggtgaaat acatctccgc
     3961 gcccgcctcg agacctctca gaagggggtg atcggccaga tcctccaggg cgttccagcc
     4021 catgtgcggg atcttggcgg cggggtcggc cgggtgcagc cgggcgacgt cgccgggaat
     4081 ccagcccagg cccggggtct cgccgaactc cagtccgcga tcggccagca gctgcatgcc
     4141 gacgcagatg ccgaggaacg gacagccccg gacgatggcc gtctccgtca tggccgcgat
     4201 cagcccgtcg cgcgccgaca gcgcccccat gcaggccgcg aaggcgccca cgcccggcag
     4261 gaccagccgg tcggcccggg agactacatc cgggtcggcg gtgacgatga tgtccacggg
     4321 cgtggcaagg ccttccgcgg cgcggcgaag ggccttttcg gccgagcgca ggttgcccga
     4381 cccgtagtcg atcagggcga cgctctgcat ggagatttcc tagagcgagc ccttggtgga
     4441 cggcgcgcgt ccgtgggtct ttggatccag ctccaccgcc gtccgcaggg cgcgggcgag
     4501 ggccttgaag ccgctttcgg cgatatggtg gctgttgtcg ccgtagaggg tttccagatg
     4561 cacgcaggcg cccgcgttca tcgcgaaggc gtggtggaat tccttgaaca gctcggtgtc
     4621 catggtcccg accagcggcg tcctgaaggc caccttccag atcagatagg gccggttgct
     4681 gaggtccagc gcgcagcgcg tcagggtctc gtccatcggg atataggccg agccgaagcg
     4741 gcggatcccc ttgaacccgt ccagcgccct gttgaccgcc tgcccaagga cgatgccggt
     4801 gtcctcgacg gtgtggtgca tgtcgatgtg caggtcgccg gtggtctgga cctcgaggtc
     4861 gaagccgcca tgccgcgcga agctgtcgag catgtgatcg aagaagccga tgccggtgga
     4921 gaccttcgcc tccccggttc cgtcgagatc gatgcggacg cggatctgcg tctccttcgt
     4981 ctcgcgcgtc acctcggccg tgcgggccat cagaacagtc cttccgcctt cgccagggtc
     5041 tcgagcgaga cctgcggcct ggggccgtag tggctgatca cctcggccgc ggccagggcg
     5101 cccagccggc cgcagacgtc catcgaccgt ccccgggcca gacccagcaa gaaccccgcc
     5161 gcgtattggt cgccggcgcc ggtcgtgtcc acgacttttt ccacaggata ggccggaatg
     5221 atgtgcgacg ccccgcccgc gaacaccacc gagcccgtct cgccccgggt gatggcgcag
     5281 atgcgggtgc ggtcgaccag agcctgggcg gcggcgtcga agtcgtcggt ctggaacatc
     5341 gccttcacct cggactcgtt ggccagcacg atgtcacact cggtctcgat gaaggccagc
     5401 agcacgtcgc ggtgccgttc gaccacgaac gagtcggaca gggtgatcga catcagccgg
     5461 ccggcggcgc gcgacaggcc ggcggccttg gcgaaggccc ggcgggcctc gggcggatcg
     5521 aacagatagc cctcgaggaa gatgatcctg gcgctttcga tcatccgcgg atcgatgtcg
     5581 tcggggccca gctcggtcga ggcgccgagg aaggtgcaca tcgtgcggtg accctccggc
     5641 gtgacgttga tcaggctgcg ggcggtgcgt ggtcctccga ccagcggccg ggagtcgaac
     5701 gccacgccga tggccttcat gtcgtgctca tagacctcgc ccagctggtc gtcggccacc
     5761 ttgcccagga aggccgcgcg gccgccgaaa ctggccacgc cggcgacggt gttgccggcc
     5821 gacccgcccg aggtctcgat gcccggggcc atgtggccat agagttcctc ggcctggccc
     5881 tcgtcgatca gctgcatgct gttcttcacc agtccctggc tgtcgaggaa ggcgtcgtcg
     5941 cagggggcga tcacatcgac gatggcgttg ccgatggcgg cgacgtcgta gagctcgggc
     6001 atggcggggt tctggcgttg tgaaggggac ggggctcgtt acaggacaag gcctcgcggg
     6061 gcaaacgcgg cgggcgtggc gaaaggctgt cgacttacgc cgggagagcc gttcgaggct
     6121 gccgttcctt tcaacgccgg agaacccgtc catggccgcc tacatgatct tcatccgcga
     6181 caagaccacc gacgccgccg aactggcgac ctattcaggt ctggccggcg ccagcatggg
     6241 cgggcacgcg gtgacgccgc acgtggtcta cggcccgcac gaggtgctgg aagggccggc
     6301 cgtcgagggc gtcgtcgtgg tgtcgttccc gaccctcgag gaggcgaagg cctggtacca
     6361 cagcccggcc tacagcgccg cgcgcgagca ccggttcaag ggcgccgagt atcgcgtcgt
     6421 gctggtccag ggcctttgac gccggcgccg acgtgacata ggggcgatgc atggcccgca
     6481 tcgccctgct cacccccgcc gcccacaatc cctatgtccg cgtctggggc ccggccttcg
     6541 accggttgat ggccgccctg tccggagggg ggctacaggt catcgccatg gaatggccga
     6601 aggcggccca ggccgacgtc gacgccgtcc tgcccctgct ggcctggggc tatcaccagg
     6661 acgccgccga ctggctggcg atgctcgacg cgctggaagc gcagggcgtc aaggccctca
     6721 atccggccgc cgtgttgcgc tggaacacca gcaagaccta tctggccgac ctcgaggccg
     6781 ccggtgtgcc ggtggtgccg accgttttcg tcgaccgggc cgacgatgcg gcggtggcga
     6841 aggcccgggc cggactgggc gccgacacgg tcgtggtcaa gccgcaggtg tccggcggct
     6901 cgcatgaaac agtgaagctc gcgccgggcg aggcgctggt cggcggcccc agcggcgcgg
     6961 cgatgatcca gccgttcctg ccgtcggtct ccggcgaggg cgagatctcg ctgctgtact
     7021 tcgacggcgt cttcagccac gccatcggca aggtggcgaa ggccggtgac ttccgtgtcc
     7081 agccacagtt cggctcgacc atcggcccga tcgagccgcc gccgggcgcg ctcgacgtcg
     7141 ccggcaaggt gctggccgcc gccccgggtc cgctgaccta ttgccgcatc gacctcattc
     7201 gccacccgga cgggaccctg cggctgatgg agctggaggc gatcgaacct gacctgttcc
     7261 tggagcacgc gccggaagcc ggcgcggtgt tctcggcagc ggtggcgaag gcgctgcgct
     7321 gaccgaaggg gacacgtacc ggcggtacat gtcccctatc cccccaagaa cagttcccgg
     7381 ctcggacaca ggtcccgcac ctgacactcc tcgcacttcg gcttgcgcgc caggcagaca
     7441 tagcggccat gcaggatcag ccagtggtgg gcgcgggtca ggaacggctc gggcacgatg
     7501 gccatcaggt cctgctcgac cctgtcgggc gtcttccccg tcgacaggcc cagccgatgg
     7561 gccacccgga acacatgggt gtcgacggcg atcgccggct cgatccccag ctcgttgagc
     7621 accacgctgg cggtcttgcg gcccaccccg ggcagcgcct ccaggtcggc gcggttcagc
     7681 ggagtcttgc cgccatgctt ctccaggatg atccgcgaca gggcgatgac gttcttcgcc
     7741 ttgttgcgga acaggccgat cgaattgatg tagccggtca atccggcctc tccgagggcc
     7801 agcatctgtt ggggcgtgtg ggcgaccgga aacagcttcg ccgtcgcctt gttgacctgc
     7861 acgtcggtcg cctgggccga cagggcgacg gccaccacca gagtgaaggg atcctggaac
     7921 ttcagttccg tgcgcgggtc gtccgacagg gtcaggaacc ggtcgaacag cgcggcgacc
     7981 cgcgccttct ccttcggcga caggcgtctt ttcggggcga ctttgttcat cgccggggac
     8041 catgaagcgc atcgcgccat tcgccaagcg cccgcccggc gctatacttg gatatggccg
     8101 ccgccctcta catggacgcc gaaatccggc ccaaccggtc gatgcccgcc acgggactca
     8161 aggtcctgat gggggtgatg atcgtcgcca gcgttatgct ttcgatcgtg atgttcgtga
     8221 tgggggcgtg gttcgcgccg gcctttctcg gcctcgacgt gctcggcgtc tggctcgcct
     8281 ttcgcgccag ctaccgggcc caggagcgac gcgagcgggt cagggtcacg gccgagacgg
     8341 tgacggtgct gcatgaactg gacggcgagg ccaggacggt ctggacctcc cccaccgcct
     8401 tcaccggcgt cgatgtcgac tggccgggcg agcatgaggc gcgcgtgcgg cttcgcctgt
     8461 cggcccggcg ctgcaccgtg ggcggcgccc tgagcccgga agaacgggtc gggctgggcg
     8521 aagcgttgcg cgaggccatc cgcagggccc gggcggaacg atatgcgtca tgagcgcaag
     8581 aaccgggcgg attttcgatt tcagcccgcc tagcgtcggg tcatggctta cgacatgacc
     8641 cccacccgct ccaccctggc gccggcctcc ggcgtcatca tccagctcga agagcgcgcg
     8701 cgcgactacg atcgcatggc ccgggccttg gcctggctgg ccgaccgctg gcgcgaccat
     8761 ccctcgctcg acgaggccgc cgacgcggtc ggcctgtcgc cgttccattt ccagcgggtc
     8821 ttcacccgct gggccggtgt cagcccccgg gccttcgtcg cctcgatcgc ccatgccgag
     8881 gcccgcgccc ttctggagga aggcgccagc gtgctcgacg cggccctcga cacaggcctg
     8941 tccggcccct cccggttgca tgacctgttc atcgcccagg aggcggtcac accgggcgag
     9001 acgcgacggc gcggcgaagg cctgatcctg cgctggggcc tcgcgccgac gccgttcggg
     9061 acagggcttt tcgcggtcgc gccgcgtggc ctggccggac tgggcttcgt cgacgaggcc
     9121 ggcgaggacg ccgccttcgc cgacatgcac cgccggttcc cggccgccga ctgggtgcgt
     9181 gacgacgccg ccgcccgcga gacggccatc aaggccttcg ccgagggcga gggccccctg
     9241 ccgatcgtgc tgatcgggtc gcacttccat atccaggtgt ggaaggcgct gctgcgcatt
     9301 ccggccggcg ccacggccac ctacggcgat gtcgcgcggt gggccggcaa gccgaaggcc
     9361 ttccaggcca cgggcgcggc gatcggagcc aatccggtca gcctgctgat cccctgccac
     9421 cgggtgatcg cccgcgacgg tcgcctgacc ggctatcact ggggcctcgg tcgcaaggcg
     9481 gcgatgctgg gcatggaggc cgtggcggcg gcggggtgac gcttcgccgg acctgacgct
     9541 actcggccgc cgctggcgtc cgcggtatca gcagctcgtc gaacacgatc cgccagcctt
     9601 cgggccggcg ctgccagacg cgcacatagt gcgccttgac cggcttgccg tccttgtccc
     9661 agccggccgt gccgtaggtc caggccatgt ctccggccac agagatgtcg ccgcccagcg
     9721 gggcgaaggc gatccgggcg gcgcgccagt cgagttccgg gccgaaggtc gcgcagccgg
     9781 tcgcgggcgc catcggtgag gactgaatcc ggccatcaca ggccagcacc ttcagatagg
     9841 ccgccttgct gtccaccccc gcttccgtcg cgagcgcggc ctcggccgcc ctcaccttcg
     9901 cgaaggcgcc ttcgggatag aggccaggaa ccttcggcat ggccaggtag ccgggcgcgg
     9961 ccccggggcc cggcgaggcg gccgggtcgc ttcccacgcc gccgtcgaag acccacttcc
    10021 accggccgtc ggcctgcttc ttccagacgg tgaaatagtg tccgcgcctc tggccgccga
    10081 cactgtaggg cccggtggtg aagcccaggt cgcccgaggc ggagatgccg gcccaggtcg
    10141 gccaccattc cagcttcggg gcgtcgggcc tgtcgtcggg cgaggccagg aacgtctcgc
    10201 gggcgttgac cgggtccggc gtcatcagga tggcgtcgtc ggccatgtgg gccaggaagc
    10261 ttttcttgat ccccatgaca tagccgtcgg cggcgaaggc gcgctcggcg gcgaccaccg
    10321 gttcgggggt caccggcgcc acggccgcgc gcgccggcag ggcgacgacg gccgcgaagg
    10381 ccagggtcag agcaagtcgc atggcgcacc cccggtgaac agatcgcgaa ggctacgcca
    10441 ggcggctgcc ccggaggcat gaaatcgctg taagactcct tcggcgccgg tcgtggcgcc
    10501 tgccggatcg cgccgatggc cttttccccc gccgaggtcg aacgctacgc ccgccacctg
    10561 gtcctgcgcg agatcggcgg gcccgggcag cagcgtttga aggcgaccag cgtgctgatg
    10621 gtcggcgccg gcggtctggg cgctccggcg accctctacc tggccgccgc gggcgtgggg
    10681 cggatcggcg tcgccgatcc ggacacggtc gccctgtcga atctgcagcg acaggtcctc
    10741 tacacggcgg ccgatgtcgg cctggccaag gtgacggtcg ccgccgggcg gctgacggcg
    10801 ctcaattcgg acatcgtcgt tgaaccccat gccctcgccg tcacccccga caacgccgcc
    10861 acgcttgtcg ccggctatga tcttgtgctg gacgggaccg acgatttcgc cacccggttc
    10921 gccgtctcgg acgcctgcgt ctccgccggc aagaccctgg tcagcggcgc gatcgcccgc
    10981 tggacgggcc aggtcggcgt gtttcccggc cggccctgct atcgctgtct ggtgcccggg
    11041 attccgccgg acgccgacac ctgcgcccgg gtcggcgtgg tcggcgccct cggcggggtg
    11101 atcggctcaa tgatggcgct cgaggcgatc aaggtcatca ccggggccgg tgagcccttg
    11161 tccggccggc tgctgatcta tgacgccctg gccggcgaga cccgtaccgt gcgcatcggg
    11221 tccgacccgg gctgcccggc ctgccacgct tgacgaacgg gacgcgagcg ggcgtcaatc
    11281 cgtccgtcgg ccggcaagag ccgggtctgg ggcggaacgc aagatgaaga cgctgggcag
    11341 gatcctgatc ggggtcgtgg tgatcatcgc cgtcgtgctg accggcggct ggctggtcct
    11401 gcgccgccct gacatcccct ggccgaccct ccaggcgaag tacgccaatt ccgcctcccg
    11461 ctacatggac ctgcccggcg ggctcaaggt gcactaccgc gaccagggaa ggcgcgatgg
    11521 cccggtgatc gtgctggtcc acggcttcgc cgcctcgctg cacacctggg agccctgggt
    11581 ggcgcgcctg ggatcggaat accgcatcat cacgctcgat ctgccgggct tcggcctgac
    11641 cagcgccccg gacggctaca ccgtggcgcg gagcgatttc gtcgacgtgg tcgatcggct
    11701 gacggcaaaa ctgggcgtca cgaagttcgt cctcgccggc aattccatgg gcgggggcgt
    11761 cgcctggaac tacgccctga aacacccgga caagctggag ggcctggtcc tggtcgacgc
    11821 cgccggatgg ccgcagcagc gggacgatgg acgcgacggt ccgttcatct tcaaggtgtt
    11881 gcgcaatccg gtgggtcgtt tccttgtccg ggacctggac gtcagcggta tgacccgggc
    11941 agggctcagg gacgccttcg ccccgactcc ggaaatggtc gatgacgcca tggtcgcccg
    12001 ctatgtggag atgtcccgcg ccccgggtca tcgcgacatc gtgctgacga tgatgggcgg
    12061 ctatgatcct gccgacgcgg cttcgaagga gaagctggcg aaaatcgtcg tcccgaccct
    12121 ggtgatgcac ggcgacaccg accggctgat cccggtcagc cacgccgccc tgttcggcca
    12181 ggccattccc gggtcgacgg tgatcatcta tccgcgggtc ggccacgtgc cgatggagca
    12241 gatcgccgac aagtccgcgg ccgatctcga cgcctggctc cgggccaggg tctggcccag
    12301 gcccccggtc aaccgctgaa gccttcgtcg atgaacgccg tcgtctccag caggatctga
    12361 ctgagcacgt cgggctccca tccgccgtgg ccggcattgc gcagctcgat cagccgcgcg
    12421 ggccggttct cgcgcttgag gctcgccgcc atcgcccgcg actgccggat gggcacgacc
    12481 ccgtcgacgt cgccgtggat cagcagcacc ggcgcggcga gctcggccgc ccgttcattg
    12541 ggcgagccgc gtttcaatgc ctcacggtcg gtcgccggat cgccgaccgt cttgacgaaa
    12601 tagagatagc tgggggagtc ggcgccgtcc tcgcgctcat agctgagcag gtcgggcagg
    12661 tcggtgaccc cggcgatcga gacgatcctc cggtacaggt ccggcttgcg aatgccgccc
    12721 atcagggcgg cgtagccgcc atagctggcg ccgcagatgg cgatcctgcc gggagcgacc
    12781 gcgccgctgg ccacgacatg ggcgacgcag tcctcgacgt cttcctgcat gcggtcggcc
    12841 cagcgacggt gtccggcctc ggcgaacagc cgaccgtagc cgctggagcc gcggaaattc
    12901 ggctgcagca ccagccagcc cttcgatgcg aaggcctggg cgaacaggtc gaaggcgaag
    12961 ctgtcgcgcg catgggggcc gccatgcggc atgaccacca tcggccgcgg tcccgtcgcc
    13021 agtggccgcg tcagataggc ggccagctcc acgccgtcgc gcgagcgcac cgtcaggacc
    13081 tccatcggag ccagccgctc cgctggcagc catggcttct gccggcccaa caggtcgagc
    13141 gagcgggtct cccggtcata gagatagaag gcgccggact cccggggccc cgaagcgtgg
    13201 acaatgaacc ggcgccggcc ggcgtcgata tcgtgcagca gcacgttggc gtccgcgccc
    13261 agggcgttct ggactgcgcg gtagtggggg gccagggccg cgtcgaggaa ctcgtactgc
    13321 gtgcggttct cggtataggc ggccgcgacc ggattgaggc ccttgtcgaa gaacacccct
    13381 tccatgtccc ggcccgcatg gctggccacc acctcgccaa gggcgagggt gcggatgtcg
    13441 aagcggcgaa tggtccgcgc atcatcgccc ggcgccatgg tcgccaccag cagcacgccg
    13501 gcctcgtcgg tcgcgccgac cgcttcgaag tcgtcgaatt gcgagaagtc gttgctgcgg
    13561 atcttgcgca cgaactgcca gtcccgcgcg ccgggcggcc gggcggaaag gcgggtgtag
    13621 cggcccagtt cgctctgatc gacccgcagc accgcgacgc cgttctgggt gtaccagaaa
    13681 cgcgtccgct cgctgccgcg ttcgtgcagc tcgcccttgc cggtgtggat gttcaccttc
    13741 tgcagggtgt tggcgtacag gtccgggtcc cagacctgca tcaggatatg gtcgtcgtcg
    13801 ccgaccatcg ggtccaccac atgggagaca ttccagatgt agctctgggc gctccggtcc
    13861 tggcccagca ggaccaccgg gttgccgcca tcggcgccga tggccatcag ccggtgaacc
    13921 gaccgctcat agagtcgcgc gcccgcccgg atgccggtct tcctgccgtc ggcggtcttg
    13981 tcgaagctga accagaccag cagccggtcg ttgctggccc actgcacgcg atgcacgtcg
    14041 tagtcgccga tgacggcctg aatcggcggc gcccgcatgt ccgcgacctt cgccagcagc
    14101 acgtaggaca gccgcctgtc cccctcgcgc tgctcgcgaa gcagggcgat gcggtcgccg
    14161 gtcggtgaca gggccgcgtc cagcacgacg gcctcgcgcg tcaggtcttc gagggtgggc
    14221 ggcgcggcgc cggacgcgac ctccgcggcc agggccggaa cccctgcgaa cgctaccggt
    14281 ccggccgccg cgagggcgac cagccttctg cgtgtaatca tctacgcccc cgacaccgtt
    14341 tccccgcgac aaccttgacg gcgcatgcgg ggcggcgtca accggtcaga gcatcgcggg
    14401 gatcacccgg tcgggcggcc ggtggccgtc catgaaggtc ttgatgttga cgatgacctt
    14461 ctcgcccatg tcgatgcggc cttcgaccgt ggccgaaccc atatgcggca gcagcaccac
    14521 attgggcagc ttgagcagct tgggattgat cgccggctcg tgctcataga cgtccagtcc
    14581 ggcgccggcg atctcgccgc gggccagcat gttggccagg gccccctcgt cgatgacctc
    14641 gccacgggcg gtgttgacga tcaccgcggt cggctgcagc agcttcagcc ggcgggccga
    14701 gagcaggtga taggtcgccg gggtgtgcgg gcagttgatg gagacgatgt ccatccgcgc
    14761 cagcatctgg tccaggcttt cccagtaggt cgcctccagc tcctcggcga cgcgcgggct
    14821 gaccggcttg cggttgtggt agtggatcga caggccgaag gccttgccgc ggcgggccag
    14881 cgcctggccg atacggccca tgccgatgat ccccagccgc ttgccccaca ggcggcgacc
    14941 gagcatccag gtcggcgacc agccctggaa gccgcccgac tgaaccacgt tggcgccttc
    15001 gacgatgcgc cgggtcgagg ccatgatcag gctcatggtc aggtcggcgg tgtcctcggt
    15061 caggaccccc ggggtgttgg tgacgatcac gccgcgctgg ttggcggtgg ccacgtcgat
    15121 gttgtcgacc ccggccccga agttggcgat cagcttcagc cgctcgccgg cgcgcgacag
    15181 caggcgcgag tccaggcggt cggtgatggt cggcaccaga acgtcggcgc ggcccatggc
    15241 ctcgaccagt tcgtccggcg tcatcggccg gtcatcgacg ttgagttcgg tgtcgaacag
    15301 ctcccgcatc cgcgtttcca ccggatcagg cagcttgcgt gtgacgacga ctttcggctt
    15361 cttggcggcc atggacgctt tcgataacgg cgttggcccc tcggggggtc gtcgcaccct
    15421 tagcaaaggc ccggcggggg gccaagggaa gctgcgaccg ttcagggccc cagattggtc
    15481 tttgccggta aagttggcgc tgatcgccct ggcggtgacg ctcgccggtt gcaacgggcg
    15541 gggggcccag gtctgcgaca cgccctcgac cctgccggtg ccgcgcttcg tctcgctgaa
    15601 gtttggcgag gtctacgccc gcaaggggcc gggtgaggac caccggattc tctgggtctg
    15661 gcgctcgaag ggcatgcccc tggaggtggt cgccgagacc agcgactggc ggcgggtccg
    15721 cggacccgac ggcgccctgg cctgggctca caagcgaggg ctcgacggcc ggctcacggt
    15781 gatgcgcgtg cggccgggcc ccttgccgat gcgcgccgcc c
//
LOCUS       DAORNX010000011        15525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_2897_length_15525_cov_7.647964, whole genome shotgun sequence.
ACCESSION   DAORNX010000011 DAORNX010000000
VERSION     DAORNX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_15525_cov_7.647964"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLO65_00985"
     CDS             <1..180
                     /locus_tag="PLO65_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26854.1"
                     /translation="SGRRRGRGQRRRQPQDQDTERYRHPASIPLFERKINGRRVTNGQ
                     RRRMLAARNRPPRGP"
     gene            180..548
                     /locus_tag="PLO65_00990"
     CDS             180..548
                     /locus_tag="PLO65_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRD26855.1"
                     /translation="MISPDQVRAFALALPEVEDKSTSGHLAFAVAGKGFAWSWMERAR
                     VGARRQPRLDVLAVRAEEKEMIVEADPSAFVCDDHYRGFPAVLVRLDRIAEGDLAALL
                     AVAWRCQAPRALIRAVDAGR"
     assembly_gap    726..762
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<763..1040)
                     /locus_tag="PLO65_00995"
     CDS             complement(<763..1040)
                     /locus_tag="PLO65_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HRD26856.1"
                     /translation="MTARPLARLTVAVTRLPTLDRPAFQAYWRERHGPLVRRLAGTLG
                     IVGYVQLHADLEDEGPDDPARRPWSAPCDGLAQIWYASRQDFEARMTDP"
     gene            complement(1037..2557)
                     /locus_tag="PLO65_01000"
     CDS             complement(1037..2557)
                     /locus_tag="PLO65_01000"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD26857.1"
                     /translation="MPSEASPAAEHFDVVIIGAGITGIDGAYHLATQCPGRTFVVLEG
                     LESFGGTWLTHKYPGIRSDSDLYTFGYRFKPWIGPPIATAAEIRKYLGEVIAENGLAD
                     RMRYNHRIGSARWSSESNLWTVEGVRTDTGQPVRFTCNFLWMCQGYYRHAEGYTPEWE
                     GMDTFRGRIVHPQTWPDDLDYAGKRVIVIGSGATAATVVPAMAADCEHVTLLQRSPTY
                     FFPDHNRNRLADTLREVGIEEALIHDIVRRKIVHDQYRFVSRALDDPEAVKQQLLAGV
                     AAHLPQEVVDEHFTPTYKPWRQRIAFVPDGDLFEGIRSGKASVVTDHIDRFTAKGILL
                     KSGRELEADIIVTATGFNLNVLGDIDFSIDGQTLNFAETITYRGMMFTGVPNMLWVLG
                     YFRGASWTLKADLVADFVCRLLNHMAATGASRVSVALRPEDRDMALLPWIDPDTFSPG
                     YLMRGVHLLPKRGDKVEWAHSQDYWREKDELPRIDLDDPVFVYQRKAAAARTAPAA"
     gene            complement(2603..3472)
                     /locus_tag="PLO65_01005"
     CDS             complement(2603..3472)
                     /locus_tag="PLO65_01005"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD26858.1"
                     /translation="MSGDRLAGRTAVITGGASGIGFEVARRFLSEGASVVIGDYNAEA
                     GVAARTRLAGEGFDGRVAFIRTDVCQEDDVEALMAGAVDRFGGLDIAFNNAGIAGAVG
                     PIVETHADHWDATFAVMTRGVFLGVKHAARLMIPRGGGSIINTASIAALSSGVVPTAY
                     SASKAAVVSFTRNAATELAEHRIRVNAVCPGIIFTPLMHNGREEQAEAVVREIQPWPD
                     RGEPRHVASAVLYLASDESAFVTGEAHTVDGGYLANGLLRVHPLPGAKKKPDYAGITY
                     GMTGQAREIRRLG"
     gene            complement(3630..4703)
                     /locus_tag="PLO65_01010"
     CDS             complement(3630..4703)
                     /locus_tag="PLO65_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRD26859.1"
                     /translation="MPSLFDPVTLGAIVAPNRILMAPLTRTRGTREHAPTDLMARYYA
                     QRASAGLIVAEAIGVSRQGLGWPYATGLWRDDQVSGWRRVTAAVHEAGGRIVAQLWHM
                     GRMVHPSFVDGASPVSASATRAPGRAHTYYGREPYAVARPLTREEIPGLVEDYRRAAA
                     NALVAGFDGVQVHAANGYLIDQFLRDNANLRTDGYGGAVENRIRLLCEVTRAVAGVAG
                     ADRTSVRLSPNGDSQGVNDSDPEPLFRAAGAALSDIGIGFLELREPPRDGSLGQGDRD
                     PLAPAIREAFNGPLVLNSDYDQARAEATLASGQADAISFGRLFIANPDLPRRFAEGAP
                     LARDDKLTWYSQGAEGYLDYPAA"
     gene            4951..5970
                     /locus_tag="PLO65_01015"
     CDS             4951..5970
                     /locus_tag="PLO65_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD26860.1"
                     /translation="MTGQRRLRMGILGCARINRAALLDVRPFAPQIEVLAVASRDTQR
                     SAGYARAHDVPRAYGDYQALLDDPDIEAIYNPLPNSLHADWTIRALEAGKAVLCEKPM
                     ASNADEARRMADAAQAAGRPLVEAFHYRHHPLALFVADLFRSGDLGAVRAIDAGLCIP
                     GDLVPPDDIRFQSDLSGGAMMDVGAYCMNILRLVAGEEPMITAASAASVTQGVDRAMR
                     AGMTFPSGATGSLDCSLAADGFRAWLTVDAERGRLEIDNPFLPHLGHRLVLETDGQRR
                     EQTCDPTPTYVFQARAFAGTVLAGGEIRTTAADGVANMTAIDAVYRAAGLAPRAGQVL
                     AGQTI"
     gene            complement(5950..6762)
                     /locus_tag="PLO65_01020"
     CDS             complement(5950..6762)
                     /locus_tag="PLO65_01020"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD26861.1"
                     /translation="MAHQKTRDTGRFVDQVVVITGAASGIGRASALRLAAEGADIVGL
                     DLSSEGLATLGGEVAALGGRCETVVGSVADLATIDAAVTRAVEAFGGLDGLVNNAGIG
                     GPMKRLDAVEPDEFDAIVAINLRSVWYGMKAAYAPMRARGGGAVVNVSSMAGLRPNRH
                     HSPYGMTKAGVISLTQHAAMDFASARIRVNCLCPGPVETPIFEQMERNLDAEAYRVAR
                     QRVQQRTLMNRFGTAEEQAAAVAFLLGPEAGFITGIAMPVDGGWSVSDGLAR"
     gene            7018..8223
                     /locus_tag="PLO65_01025"
     CDS             7018..8223
                     /locus_tag="PLO65_01025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD26862.1"
                     /translation="MTRTGDTDSRDPRPGPLAGIRIIDLSQFILGPYATQILGDLGAD
                     VIKVEEPSGDRQRQSGKAPRSKEMGPGYVAFNRNKRSVSLDLKTEAGKDTLRQLVATG
                     DVFIHNMRPAAVARLGFSHAQVAALKPDIVYVEAMGYDDAGPYAGLQAFDDLIQSASG
                     ACGLGQLVDPDAELRPLPSFLADKTCGLFAVIATLAALRHRDQTGEGQYVAVPMLETF
                     TGFLMAEHLYGETFVPPTGGFGFTTAVTPHRRPLKTRDGYLTVLPASAEQSARFMELG
                     GLPGAYESERFTSKPSGREKVAEYYAMMGEAAASRTTEEWMALGAAHSIPVMRANTPK
                     MIFEDEQLALTLFEARELPGEGTYRAMKPGLRFSKTPASIRRDPPRIGQDTDEVLAEL
                     GLPPPPGDA"
     gene            8229..8975
                     /locus_tag="PLO65_01030"
     CDS             8229..8975
                     /locus_tag="PLO65_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRD26863.1"
                     /translation="MPDQPPSPARPPPADSPARRARPELSRDRILSTALELIDAHGLN
                     TLNMRDLGQALGASTMSVYRHFRNKAELLDAVVDHVVEGFAPPAMDGDWQAQARTLSL
                     RVRAGMLAHPELADLIGREFRRSPTSLRVNAAIIERLRQAGVPRALLPDTYWAISSYT
                     NGYALLEAQSRRRRRRPPAPGAEVGRVRKLAAMLDEVEGLDPQVSQAAAVVLSRPLDD
                     RQFLFGLDCLIAGIEERIRQARATGDQAEA"
     gene            complement(8960..10099)
                     /locus_tag="PLO65_01035"
     CDS             complement(8960..10099)
                     /locus_tag="PLO65_01035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD26864.1"
                     /translation="MSEPMIGGDVDPAFAAVADAFADNFRNEGEIGASICVFQAGRKV
                     VDLWGGHADAARTRAWGPDTIVNVWSTTKGMMALCVARLVDQGLLANDRPVADYWPEF
                     AANGKGAVTVAQLFSHQAGLCGPGRPLTEAELLDTDFVADLLAAEAPHWPVGTRSGYH
                     ALTIGPLGDGLFKRVVGKTVGQYFRDEIAGPLAVDFHIGLPIEEEGRVAEIVHDGNPE
                     SGGPESFNAYQRLAQQNVPVRSGIANLRAWRAQGTPSAAGTGNARGLATVYSALATDR
                     RLGGVELVSESALAAATAIQIENEDLVLRFPMSWGIGFAVNKAMGVYGTSPRAFGHHG
                     WGGSFAFADPDSGLAVAYAQNFMREPQGGLDPRFAALVGAVYASA"
     gene            complement(10173..12575)
                     /locus_tag="PLO65_01040"
     CDS             complement(10173..12575)
                     /locus_tag="PLO65_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26865.1"
                     /translation="MDHHSRSTVSSRRAWRHASGQTRAAGPLLGLLLTGVSMAAVSTA
                     PALAQADPAAVGVTVQEIVVTANKREETANTVPMSITAATGEVLERQGIDQPRDLVKI
                     TPSFTYADSYVGSPIFTLRGVGFSDISLGGRPTVSIYIDEAPLPFSIVTRGAGMDLER
                     VEVLKGPQGTLFGQNATGGAINYVAAKPTRTFEAGANLGFSTFDTVEAGGFVSGPLGD
                     TASVRLAIDHSHGDGWQKSYTTGQTNGALDFTTGRMILAWTPSEQLRVQLSINGFQDR
                     SDVQAGQLIAISPGIPAAAPFVPGLLTYPLAPANARAADWNPGLDYARDNAFVQGNLR
                     IDYDLTDSLTLTSLTSVSHYDHRELQDIDGTTLSNLSQLTTGEIASISQELRLAGEIG
                     VAGHFVLGATYARDEVDQVGFDRIDQSTTAFTFVPFGLPLFTSFVDSNSQKVRTTAVF
                     ASADYQVVDTVKFYGGLRYTRSVDKFHGCTADSGDGVAALDFGVLQNVFRAGAGLPPN
                     PPIPAGGCVTSDAAFVPGLVRDTLDEDNVAWRAGAEWTPRPRMLLYANVSRGYKAGGF
                     PDLAATLDTQYLPATQESLVAYEAGFKTTLLERTLQLNGAVFYYDYSDKQILGKVLDP
                     VFGPLLRMVNVPKSEITGAEIQAVWTPARGLTINAGGSYIDSRVLDHFTNYDAAGALT
                     DFHGEAFPNTPKWQYVMDIDYRRDVAEGLVGFVGGGVTYQSETNSQLGELPALAVDSY
                     TLVDLRAGLESTDGAWRVSAWGRNVGDVYYWTAANRNLDTTVRFAGRPATYGVTLSYR
                     FD"
     gene            complement(12742..13728)
                     /locus_tag="PLO65_01045"
     CDS             complement(12742..13728)
                     /locus_tag="PLO65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD26866.1"
                     /translation="MCLADIRRYLGADGKSPERVAALLQAHGIDDGAGRSDALVPIDV
                     CWRIFTEHAALIDDEMHCAFGTRLKPGGTNLIIARMLLCPTIHEALCAYAEASGIIVQ
                     ELQVTVARRKDGLSLRWRSVHPDNPLHQIVLEGMAAVYYSILCWMAGDALPVLRVRAP
                     ASRRNGASTLLHLMGAPVVYGGNDLEIVFAPEVARTAVGSVEIGAWRDGAYRALSTLA
                     RRVRPNTLGGAFSDKVRSALLDGVDQESVARRWGVSTKTVARRLEQEGCSFRRIRDEV
                     RMQNSMCLIHAGLTVEQIGERLGYEDSRSFRRAFRRWFGLSPSAYRSQHAPA"
     gene            complement(13952..>15525)
                     /locus_tag="PLO65_01050"
     CDS             complement(13952..>15525)
                     /locus_tag="PLO65_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26867.1"
                     /translation="EGMVNLPLFGGRGAVRAVAWREQAGGYIDDAGLGLTNVNRTVRL
                     GGRLAASVELNDRWSLSAGIVGQAIDSADTQYALAGEPPYTRRNSLREPHDNDFAEIN
                     ASLRGDLGWARLNWSTAFVDHRLDSRYDATIAPPVPMPPGPAAFDDENGIVSVVTEAT
                     LLSPAEARVGWLAGLFYARARQDVTLSLARPGAGGPPALNENRRDRLDEIAAFGEVTL
                     PLADRLELTLGGRLFNVAGRTSSRIAASPGAVSIYQGSSRQTGLAPKILLAWRPSSTR
                     LWYLTAAEGYRAGGINTTGAPGQPFSAPGGPQPWRRYQGDELWSFEAGGRFQWLDGRL
                     QMTVAAFEARWSNIQSDQLLPNGLPFTANIGDGVNLGIEVEGVYRAGALVLTGNALFN
                     APELDRANAGFTSRGDLALAAVPELSGGLSAHYAWPLAGERLFALDARLAYVGRSTLT
                     FDAATSPDMGDYLDGRLAGSLVTDHWTLTLAVTNPADSRGDTFAYGNPFTLKGARQMT
                     PLRPVTAEITVRASF"
BASE COUNT         2321 a         5410 c         5486 g         2271 t
ORIGIN      
        1 agcggccgcc ggcgcggccg cggccagcgc cgtcgccagc cccaggacca ggacacggag
       61 cgataccgtc atcccgcctc gatcccgctt ttcgagcgca agatcaatgg ccggcgggtg
      121 acaaacggcc agcggcgacg gatgctggcc gccaggaacc gcccgccaag agggccgtga
      181 tgatctcacc cgaccaggtt cgcgccttcg ccctcgccct gcccgaggtg gaggacaagt
      241 cgacctccgg acacctcgcc tttgccgtcg ccggcaaggg cttcgcctgg agctggatgg
      301 agcgggcgag ggttggcgcg cgccgccagc cgaggctgga cgtgctggcc gtgcgggccg
      361 aagagaagga gatgatcgtc gaagccgacc cgtcggcctt cgtgtgcgac gatcactacc
      421 gtggctttcc cgccgtgctg gtgcggctgg accgcatcgc cgagggcgac ctcgccgcgc
      481 tgctggccgt cgcctggcgc tgccaggcgc ccagggccct gatcagggcg gtcgatgcag
      541 ggcggtgagc gtggccggcg caagaggcgg gaaggctccg agaaaaaaag tcgtccggaa
      601 ctggtcccga acgacagttg cgcgccggga ggtcatggcg cgggagggga tgaagacggc
      661 ggctagagga tcgggtgctc gacgccccac cagcggcggg tccgggcgcg atccatgaac
      721 agcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggtcggt catgcgcgcc
      781 tcgaagtcct gccggctggc gtaccagatc tgcgccaggc catcgcaggg ggccgaccag
      841 gggcgccggg ccggatcgtc cggcccctcg tcctcgaggt cggcgtggag ctgaacgtag
      901 ccgacgattc cgagggttcc ggcgagacgt cgcaccaggg gaccgtggcg ctcgcgccag
      961 taggcctgga acgccgggcg atcgagcgtc gggagccggg tcacggcgac cgtcaggcgg
     1021 gcgagcggcc gcgcggtcat gccgccggag cggttcgggc cgccgccgcc ttccgctggt
     1081 acacgaagac ggggtcatcg agatcgatgc gcggaagctc gtccttttcc cgccagtagt
     1141 cctggctgtg ggcccattcg accttgtcgc cgcgctttgg cagcagatgc acgccgcgca
     1201 tcaggtagcc cgggctgaag gtgtcgggat cgatccacgg cagcagcgcc atgtcccgat
     1261 cctccggccg caacgccacc gagacccgcg acgcgccggt cgcggccatg tggttgagca
     1321 accggcagac gaagtcggcc accaggtccg ccttcagcgt ccagctggcg ccgcggaagt
     1381 aaccgagcac ccacagcatg ttcggcacgc cggtgaacat catcccgcga taggtgatcg
     1441 tctcggcgaa gttcagggtc tgcccgtcga tgctgaagtc gatgtcgccg agcacattga
     1501 ggttgaagcc agtggcggtg acgatgatgt cggcctccag ctcccggccg gacttcagca
     1561 ggatgccctt cgcggtgaag cggtcgatat ggtctgtgac caccgacgcc ttgccagacc
     1621 ggataccctc gaacagatcg ccatcgggca cgaaggcgat ccgctggcgc cagggcttgt
     1681 aggtcggggt gaagtgttca tcgaccacct cctgcggcag atgggcggcg accccggcca
     1741 gcagctgctg cttgacggcc tcgggatcgt cgagcgcccg gctgacgaag cgatactggt
     1801 cgtggacgat cttgcgccgc acgatatcgt ggatcagcgc ctcctcgatg ccgacctccc
     1861 gcagcgtgtc ggccaggcga ttgcggttgt ggtcggggaa gaagtaggtc ggcgaccgct
     1921 gcagcagggt gacgtgctca cagtcggcgg ccatggccgg aacgaccgtc gcggcggtcg
     1981 cccccgagcc gatgacgatg acccgcttgc cggcgtagtc gagatcgtcc ggccaggtct
     2041 ggggatggac gatcctgccc ctgaaggtgt ccatgccctc ccactccggc gtgtagcctt
     2101 cggcgtggcg gtagtagccc tggcacatcc agaggaagtt gcaggtgaag cggaccggct
     2161 ggccggtgtc ggttcgcacg ccctcgaccg tccagagatt gctttcgctc gaccagcgcg
     2221 ccgagccgat ccggtgattg tagcgcatcc ggtcggccag gccgttctcg gcgatgacct
     2281 cgcccaggta cttgcggatc tcggccgccg tcgcgatcgg cggaccgatc cacggcttga
     2341 agcgatagcc gaaggtgtag aggtcgctgt ccgagcggat gcccggatac ttgtgggtca
     2401 gccaggtgcc gccgaagctc tccagcccct cgagcacgac gaacgtcctg cccgggcact
     2461 gggtcgccag atgataggcg ccgtcgatgc cggtgatgcc ggcgccgatg atcacgacgt
     2521 caaagtgctc ggcggcgggc gaggcctctg aaggcatgga cttttccctg gtcattgttg
     2581 tcgtggtcgg cggcgggcgc ggtcagccga gcctgcggat ttcgcgggcc tggccggtca
     2641 tgccgtaggt gatgccggcg tagtcgggct tcttcttcgc gcccggcagc gggtgcacgc
     2701 gcaggaggcc gtttgcgagg tagccgccgt cgaccgtgtg cgcctcgccg gtcacgaagg
     2761 cgctctcgtc gctggccaga tagagcaccg cgctggcgac atggcgtggc tcgccacgat
     2821 ccggccaggg ctggatctcc cgcacgaccg cctctgcctg ttcctcgcgg ccgttgtgca
     2881 tgaggggcgt gaagatgatt ccgggacaga cggcgttgac gcggatgcga tgctcggcga
     2941 gttcggtcgc cgcgttgcgg gtgaagctga ccaccgcggc cttgctggcc gaataggcgg
     3001 tcggcacgac accgctcgag agggcggcga tcgaggcggt gttgatgatc gacccgccgc
     3061 cgcgcgggat catcagccgc gcggcgtgct tgacccccag aaacacgccc cgggtcatga
     3121 ccgcgaaggt cgcgtcccag tgatcggcat gggtctccac gatcgggccg acggcgccgg
     3181 cgatgccggc gttgttgaag gcgatgtcca gcccgccgaa gcggtcgacg gcgccggcca
     3241 tcagggcttc gacatcgtcc tcctggcaga catcggtgcg gatgaaggcc acgcgcccgt
     3301 cgaagccctc gcccgcgaga cgggtccggg cggcgacgcc cgcctcggcg ttgtagtcgc
     3361 cgatcaccac gctggccccc tccgacagga agcgtcgcgc gacctcgaag ccgatgccgc
     3421 tggcgccgcc ggtgatcacc gccgtccgcc ccgcgagacg atcgccgctc atgttaggga
     3481 cccggcttgg cggaccgccc gggcgttccg ccggaactcg ataggcatgg gatcaactct
     3541 tcggtctgtc ctgttgtggc ccccaccgta ggagcgaccg gcggcgccaa acaggtcccg
     3601 acagcccggc cgggggcgga cacttttggt caggcggcgg ggtagtcgag gtagccttcc
     3661 gcgccctggc tgtaccaggt gagcttgtcg tcccgggcca gcggcgcgcc ctcggcgaag
     3721 cgccgcggca gatccgggtt tgcgatgaac agccggccga agctgatggc gtcggcctgg
     3781 ccggacgcca gcgtcgcctc ggcccgggcc tggtcatagt cggaattgag cacgagaggg
     3841 ccgttgaacg cctcgcggat cgccggcgcg agggggtcac ggtcgccctg gcccaggctg
     3901 ccgtcccggg gcggctcgcg cagctcgaga aagccgatgc cgatgtcgga cagggccgcc
     3961 ccggcggccc ggaacagcgg ctcggggtcg ctgtcgttga cgccctggct gtcgccgttg
     4021 ggagagagcc ggaccgaggt gcgatcggcg cccgcgacgc cggccacggc ccgggtcacc
     4081 tcgcagagca gccggatgcg gttctcgacc gcgccgccgt aaccgtcggt ccggaggttg
     4141 gcgttgtcgc gcaggaactg gtcgatcaga tagccattgg cggcatggac ctggacgccg
     4201 tcgaaccccg ccacgagcgc gttggcggcg gcgcgccggt agtcctcgac gaggccgggg
     4261 atttcctcgc gcgtcagggg cctggccacc gcgtagggct cgcggccgta gtaggtgtgg
     4321 gcccggccgg gcgcccgggt cgcggatgcc gacaccggcg aggcgccgtc gacgaaactg
     4381 gggtggacca tccggcccat gtgccagagc tgggcgacga tgcgtccgcc agcctcgtgg
     4441 accgccgccg tgacgcggcg ccagccggac acctggtcgt cgcgccagag cccggtcgcg
     4501 tagggccagc ccagaccctg ccgactgacg ccaatggcct cggcgacgat cagcccggcg
     4561 ctggcgcgct gggcgtagta ccgcgccatc aggtccgtcg gcgcatgctc gcgcgttccc
     4621 cgggtccggg tcaggggcgc catcaggatg cggttgggcg cgacgatcgc gccaagtgta
     4681 acgggatcga agagggaggg catccggggt gaccatcgct gcgtggggtt ggcgaagcgg
     4741 gcgcaccgga tcggccgcgc ggctcctgag cgcggacgtc gggtcccgcc cgcagcccga
     4801 ccctagaggt tcgacttccg cccgaaaggg tcgaaccggc cgggggccgg cggacatttc
     4861 cggccaggcc agcggtccgc cgcgatggca tgtttacatc gtacacatcc tgtcgcagcc
     4921 tgagcccggc ttcgcgacga gggggacgac atgaccggac aacgacggct gcggatgggc
     4981 atcctgggct gcgcccgcat caaccgggcg gccctgctgg atgtccggcc cttcgcgccg
     5041 cagatcgagg tgctggcagt ggcttcgcgc gatacgcaac ggagcgcggg ctacgcccga
     5101 gcccatgacg tgccgcgcgc gtacggggac tatcaggccc tgctcgacga cccggatatc
     5161 gaggcgatct acaatccgct gcccaacagc ctccacgccg actggaccat ccgcgccctt
     5221 gaggccggca aggcggtgct gtgcgagaag ccgatggcgt ccaacgccga cgaggcccga
     5281 cgcatggcag acgccgcgca agccgccggg cggccactgg tggaggcctt ccattatcgc
     5341 caccacccgc tggcgctgtt cgtcgccgac ctgttccgct cgggcgatct gggcgcggtg
     5401 cgcgcgatcg acgccggcct ctgcattccc ggcgacctgg tcccgcccga cgacatccgc
     5461 ttccagtctg acctgtccgg cggcgcgatg atggacgtcg gcgcctactg catgaacatc
     5521 ctgcgcctcg tcgccggcga agagccgatg atcaccgccg cctcggcggc gtccgtgacg
     5581 cagggcgtgg acagggccat gcgggccggc atgacgtttc catcgggcgc gaccggctca
     5641 ctcgactgct cgctggcggc cgacggcttc cgggcctggt tgacggtgga cgccgagcgg
     5701 ggccggctcg agatcgacaa ccccttcctg ccgcacctcg gccatcgcct tgtgctggag
     5761 accgacggcc agcggcgcga acagacctgc gatccgaccc cgacctacgt cttccaggcg
     5821 cgcgccttcg ccgggacggt gctggccggc ggcgagatcc ggaccacggc cgccgacggc
     5881 gtcgccaaca tgacggcgat cgacgccgtc tatcgcgcgg ccgggctggc gccgcgcgcg
     5941 ggacaggtcc tagcgggcca gaccatctga aacggaccat ccgccatcga ccggcatggc
     6001 gatgccggtg atgaagccgg cttcgggacc cagcaggaag gccacggcgg cggcctgttc
     6061 ctccgccgtg ccgaagcggt tcatgagcgt gcgttgctgc accctctgtc gcgccacccg
     6121 ataggcctcg gcgtcgagat tgcgctccat ctgctcgaag atcggcgtct cgaccgggcc
     6181 cgggcacagg cagttgaccc ggatccgggc cgaggcgaag tccatcgccg cgtgctgggt
     6241 caggctgatc accccggcct tggtcattcc gtagggcgaa tgatggcggt tgggccgaag
     6301 ccccgccatc gacgagacat tgaccacagc cccgccgccc cgggcccgca tcggcgcata
     6361 ggcggccttc atgccatacc agaccgacct gagattgatc gcgacaatgg cgtcgaactc
     6421 gtcgggctcg accgcatcca gccgcttcat cggaccgccg atcccggcgt tgttgaccag
     6481 gccatccagg ccgccgaacg cctccacagc ccgggtgacc gccgcatcga tggtcgccag
     6541 gtccgccacg ctgccgacga ccgtctcgca gcgtccgccg agcgccgcca cctcgccgcc
     6601 gagcgtcgcc aggccctccg acgacaggtc gaggccgacg atgtcggcgc cctccgccgc
     6661 gagccgtagc gcggaggccc gcccgatgcc gctggctgcc cccgtgatca ccaccacctg
     6721 gtcgacgaag cgacccgtgt cccgggtctt ctgatgtgcc accgcgaggt ctccgtttgc
     6781 gacgccgccg tcgcgatcct ggccagactt gattgcgtac agcgtacgct agtgcgcaat
     6841 tcggcgcttg tccaccgaag cgcgtacgat gtacgctcga aggacgactc ccggcgaggc
     6901 cctgcgttcg cgcggcgttc gcgcggcgtt cgccgggccg ggagatttcg agcgccggcc
     6961 ggggtagcct tgcgctaccg gggggacgga ccctccagca cagaagggcg acgcatcttg
     7021 acccgaacgg gcgacacaga ctctcgcgac ccccgacctg gccccctcgc cgggatccgg
     7081 atcatcgacc tgagccagtt catcctgggc ccctacgcca cgcagatcct gggcgacctc
     7141 ggcgccgacg tgatcaaggt cgaggagcct tccggcgacc ggcagcgcca gtccggcaag
     7201 gcgccccggt cgaaggaaat gggtcccggc tacgtcgcct tcaatcgcaa caagcgctcg
     7261 gtgagcctgg acctgaagac cgaggcggga aaagacaccc tgcgccagct ggtcgccacg
     7321 ggggacgtgt tcatccacaa catgcggccg gcggcggtgg cccgcctcgg tttctcccat
     7381 gcgcaggtcg ccgcgctcaa gcccgacatc gtctacgtcg aggccatggg ctatgacgac
     7441 gccggcccct acgccgggct gcaggcgttc gacgatctga tccagtcggc cagcggggcc
     7501 tgcggcctcg ggcagctggt tgatcccgac gccgagctgc ggcccctgcc ctccttcctc
     7561 gcggacaaga cctgcggact gttcgcggtg atcgccaccc tggcggccct gcgccaccgg
     7621 gaccagaccg gggagggcca gtacgtggcc gtgccgatgc tcgaaacctt caccggcttc
     7681 ctgatggccg agcacctcta tggagagacc ttcgtgccgc cgaccggcgg cttcggattc
     7741 acgaccgcgg tcacgccgca ccgccgaccg ctgaagacgc gggacggcta cctgacggtg
     7801 ctgccggcca gcgcggaaca gtcggcccgg ttcatggaac tgggcggcct gcccggcgcc
     7861 tacgagagcg aacggttcac ctccaagccc agcggccggg aaaaggtcgc cgaatactat
     7921 gcgatgatgg gcgaggcggc ggcgagccgc acgaccgagg aatggatggc gctcggcgcg
     7981 gcccattcga tcccggtgat gcgcgccaac acgccgaaga tgatcttcga ggacgagcag
     8041 ctggccctga ccctgttcga ggcgcgcgaa ctgcccggcg agggaaccta tcgcgcgatg
     8101 aagcccggcc tgcggttctc caagacgccg gccagtatcc gccgcgatcc gccgaggatc
     8161 ggccaggaca ccgacgaggt gctggccgaa ctgggtctgc cccccccgcc cggcgacgcc
     8221 tagacatcat gcccgaccag cccccgtccc ccgcccgacc gccgccggcc gactcgccgg
     8281 cccgtcgcgc gcggccggaa ctctcgcgcg atcgcatcct gtcgacggcg ctcgagctga
     8341 tcgacgccca cggcctgaac acgctcaaca tgcgcgacct cgggcaggcg ctcggcgcat
     8401 ccacgatgag cgtttaccgc cacttccgga acaaggccga actgctggac gcggtcgtcg
     8461 atcacgtcgt cgaagggttc gcgccccccg ccatggacgg cgactggcag gcgcaggcgc
     8521 gaactctcag cctgcgggtg cgggccggaa tgctggccca tccggaactg gccgacctga
     8581 tcggtcggga attccgccga tcgcccacct ccctgcgggt gaacgccgcg atcatcgaac
     8641 ggctgcgcca ggcgggcgtg ccgagggcgt tgctgccgga cacctactgg gcgatttcca
     8701 gctacaccaa cggttacgcc ctgctcgagg cccagtcccg ccgacgacgg agacgtcccc
     8761 ccgcccccgg cgcggaggtc ggccgggtca ggaagctggc ggcgatgctc gacgaggtcg
     8821 agggcctcga cccgcaggtg tcacaggccg ccgcggtcgt cctgtcccgg ccgctcgatg
     8881 atcgccagtt cctgttcggc ctggactgcc tcatcgccgg catcgaggag cggatccgcc
     8941 aggcccgcgc gaccggcgat caggcggagg cgtagaccgc gccgaccagg gcggcgaacc
     9001 gcggatccag cccaccctgc ggttcgcgca tgaagttctg ggcgtaggcc accgccaggc
     9061 cgctgtccgg gtcggcgaag gcgaaggagc cgccccagcc atggtggccg aacgcccgcg
     9121 ggctggttcc gtagacgccc atggccttgt tgaccgcgaa gccgatcccc cagctcatcg
     9181 ggaagcgcaa cacgaggtcc tcgttctcga tctggatcgc ggtcgcggcc gccagggcgc
     9241 tctcgctgac cagttcgacc ccgccgaggc gtcggtcggt ggccagcgcg gaatagaccg
     9301 tggccagacc gcgggcgttg ccggtcccgg cggccgaggg ggtgccctgg gcgcgccagg
     9361 cgcgcagatt ggcgatgccc gaccggacag gcacgttctg ctgcgccagg cgctggtacg
     9421 cgttgaagct ctcggggccg ccggactccg gatttccgtc gtggacaatc tcggccacac
     9481 ggccctcctc ctcgatcggc aggccgatgt ggaagtcgac ggccaggggg ccggcgatct
     9541 cgtcgcggaa atactggccg accgtcttgc cgaccacgcg cttgaacagg ccgtcgccca
     9601 gcgggccgat ggtcagggcg tggtagcccg atcgcgttcc caccggccag tggggcgcct
     9661 cggcggccag caggtcggcg acgaagtccg tgtcgagcag ctcagcctcg gtcagcggac
     9721 ggcccggccc gcacaggccg gcctggtggg agaacagctg ggcgacggtc accgctccct
     9781 ttccgttggc ggcgaactcc ggccagtagt cggcgaccgg ccggtcgttg gccagcaggc
     9841 cctggtccac cagccgcgcc acgcacaggg ccatcatgcc cttggtcgtc gaccagacgt
     9901 tgacgatcgt gtcgggcccc caggcgcggg tccgggcggc gtcggcatgt ccgccccaca
     9961 ggtcgacgac cttgcgcccg gcctggaaca cgcaaatcga ggcgccgatc tcgccttcgt
    10021 tgcggaagtt gtcggcgaag gcgtcagcga cggcggcgaa ggcggggtcg acgtcgcccc
    10081 cgatcatagg ttcagacatg aaacgctccg ggggaggatg aacagggcgg cgggggcgga
    10141 cgccgggtcg acggccaccc cctccgggga ggtcagtcga accggtagga cagcgtgacg
    10201 ccgtaggtcg ccgggcgacc ggcgaagcgg accgtggtgt cgagattccg gttggcggcg
    10261 gtccagtagt agacgtcgcc gacgttgcga ccccaggccg agacgcgcca ggcgccgtcc
    10321 gtcgattcca gcccggcgcg caggtccacc agggtgtagc tgtcgacggc cagggccggc
    10381 agttccccaa gctggctgtt ggtctcgctc tggtaggtga cgccgccgcc gacgaagccg
    10441 accagtccct cagcgacatc ccggcggtag tcgatgtcca tgacgtactg ccatttgggg
    10501 gtgttcggga acgcctcgcc gtggaagtcg gtcagggcgc cggccgcgtc atagttggtg
    10561 aagtgatcca gcacccggga gtcgatgtac gacccgccgg cgttgatcgt caggccgcgc
    10621 gcgggcgtcc agaccgcctg gatctcggcc ccggtgatct cggatttcgg cacattgacc
    10681 atccgcagca gcgggccgaa gaccgggtcg agaaccttcc ccaggatctg cttgtcggaa
    10741 tagtcgtagt agaacaccgc cccgttcagc tgcagggttc gctccagcag ggtcgtcttg
    10801 aagccggcct cataggccac gagcgattcc tgggtggccg gcaggtactg cgtgtcgaga
    10861 gtcgcggcca gatccgggaa gccgccggcc ttgtagcccc ggctgacgtt cgcatagagc
    10921 agcatccgcg ggcgcggcgt ccattccgcg ccggcgcgcc aggcgacgtt gtcctcgtcg
    10981 agggtgtcgc gcaccaggcc gggcacgaag gcggcgtcgg aagtcacgca gccgccggcg
    11041 ggaatgggcg gattcggcgg caggccggcg ccggccctga agacattctg cagcacgccg
    11101 aagtcgagcg ccgccacgcc gtcgcccgaa tcggccgtgc agccatggaa tttgtcgacc
    11161 gagcgcgtat agcgcaggcc gccgtagaac ttgaccgtgt cgacgacctg gtagtcggcg
    11221 ctggcgaaga ccgccgtcgt cctgaccttc tggctgttgc tgtccacgaa gctggtgaag
    11281 agcggcaggc cgaagggcac gaaggtgaag gccgtcgtgc tctggtcgat gcggtcgaaa
    11341 ccgacctggt cgacctcgtc ccgcgcgtag gtcgcgccca gcacaaaatg acccgccacg
    11401 ccgatctcgc cggccagccg cagttcctgc gaaatcgacg cgatctcgcc ggtggtcagc
    11461 tggctgaggt tcgacagggt cgtgccgtcg atatcctgca gctcgcgatg atcgtagtgg
    11521 ctcaccgagg tcagcgaggt cagggtcagg ctatcggtca ggtcgtagtc gatccgcaga
    11581 ttgccctgca cgaaggcgtt gtccctggcg tagtcgaggc ccgggttcca gtcggcggcg
    11641 cgcgcgttgg ccggcgccag cgggtaggtc agcagacccg gcacgaacgg ggcggcggcc
    11701 ggtatgccgg gcgagatggc gatcagctgg cccgcctgca cgtcggaacg gtcctggaag
    11761 ccgttgatgc tcagctggac cctcagctgc tccgacggcg tccaggcgag gatcatccgg
    11821 ccggtggtga agtccagggc cccgttggtc tggccggtcg tgtagctctt ctgccagccg
    11881 tcgccatggg aatggtcgat ggcgaggcgg accgacgccg tgtcgcccag gggcccgctg
    11941 acgaagccgc cggcctcgac cgtgtcgaag gtgctgaagc ccagattggc cccggcctcg
    12001 aaggtccggg tcggcttggc cgcgacgtag ttgatcgcgc cgcccgtggc gttctggccg
    12061 aacagggtcc cctgcgggcc cttgagcacc tccacccgct cgagatccat gccggcgccc
    12121 cgagtgacga tcgagaatgg cagcggcgcc tcgtcgatgt agatgctcac cgtcggccgg
    12181 ccgcccaggc tgatgtcgct gaacccgacc ccgcgcaggg tgaagatcgg cgatccgacg
    12241 taggagtcgg cgtaggtgaa cgacggcgtg atcttcacca ggtcgcgcgg ctggtcgatg
    12301 ccctggcgct cgagcacctc gcccgtcgcg gcggtgatcg acatcggcac cgtattggcc
    12361 gtttcctcgc gcttgttggc cgtgacgacg atttcctgga ccgtgacgcc gacggccgcg
    12421 ggatcggcct gggccagggc cggcgcggtc gagaccgccg ccatcgagac gcccgtcagc
    12481 aacaggccca gcaggggtcc tgccgcccgg gtctgtccgc tggcgtggcg ccaggcccgg
    12541 cgcgacgaca cggtgcttcg gctgtggtgg tccatgatgc ttcgctcccc attccgcgct
    12601 caccgcgggc gaccggaact ttcacggctg tggcgcagcc gacgtcccgt gttgatcacg
    12661 aagcctgggc gaacccctcc ggcctgcaca ggggcgaacg cccccgaccg caccggacac
    12721 tttcggacgg cctggccccg gtcaggcggg cgcgtgttgg ctgcggtagg cggacgggct
    12781 cagtccgaac cagcggcgga aggcgcgccg gaaactgcgc gagtcctcgt agccgagcct
    12841 ttcgccgatc tgttcgaccg tcagtccggc gtgaatcagg cacatggagt tctgcatccg
    12901 cacctcgtcg cggatccgcc ggaacgagca gccctcctgc tcgagccgcc gggccacggt
    12961 cttggtcgag acgccccagc gccgggcgac gctctcctgg tcgacgccgt cgaggagcgc
    13021 cgaccgcacc ttgtcggaga aggcgccgcc cagggtgttg ggccggaccc ggcgggccag
    13081 ggtcgacagc gccctgtagg cgccgtcgcg ccaggcgccg atctcgaccg accccacggc
    13141 ggtccgggcg acttcgggcg cgaagacgat ctccagatca ttgccgccgt agaccaccgg
    13201 cgcccccatc aggtgcagca gagtcgaggc gccgtttcgc cgcgaggccg gggcgcgaac
    13261 ccgcagcacc ggcagggcgt cgccggccat ccagcacagg atcgaatagt agaccgccgc
    13321 catgccctcc aggacgatct ggtgcagcgg attgtccggg tggaccgacc gccacctgag
    13381 cgacaggccg tccttgcggc gggccacggt gacctgcagc tcctggacga tgatccccga
    13441 cgcctcggca taggcgcaca gggcctcgtg gatcgtcgga cagagcagca tcctggcgat
    13501 gatcaggttc gtgccaccgg gcttcagccg tgtcccgaac gcacagtgca tctcgtcgtc
    13561 gatcagcgcg gcgtgctccg tgaagatccg ccagcagaca tcgatcggca ccagggcgtc
    13621 ggaccggccg gccccgtcgt cgatcccgtg cgcctggagc agcgccgcca cgcgctcggg
    13681 tgacttgccg tccgcgccca gatagcgccg gatatccgcc aggcacattc ccggcggccg
    13741 cggttcagaa tccgtatgtg acaccctcgc ctccctgcct gggccgccgc gcctcggcgc
    13801 cgccgcgacc catccccatc gcgcccgacg gcgccccggg atggcgccct gttcgtccga
    13861 cgaccaggaa agtgtacatc gtacgcaagc gcttgaacag cggccgattt gccggcgaga
    13921 tcgcgggggc gcggggcgat cgccaggggc gtcagaagct ggccctgacg gtgatctccg
    13981 ccgtcaccgg ccgcagcggt gtcatctgcc gcgccccctt cagggtgaac ggattgccat
    14041 aggcgaaggt gtcgccgcga ctgtcggccg ggttggtcac cgccagggtg agcgtccagt
    14101 gatcagtgac caggctgccg gcgagccgac cgtccaggta gtcgcccatg tccggtgacg
    14161 tcgccgcgtc gaaggtcagg gtcgaccgtc cgacgtaggc gaggcgggcg tcgagcgcga
    14221 acagccgttc gccggccagc ggccaggcat agtgggcgga cagaccgccc gacagttcgg
    14281 gcaccgcggc cagggccagg tcgccccggg aggtgaaccc ggcgttggcc ctgtccagct
    14341 cgggggcgtt gaacagggcg ttgccggtga gcaccagcgc cccggcccga tagacgccct
    14401 cgacctcgat cccgaggttg acgccgtcgc cgatgttggc ggtgaacggc aggccgtttg
    14461 ggagcagctg gtcactctgg atgttcgacc agcgggcttc gaaggccgcc acggtcatct
    14521 gcaggcggcc gtccagccac tggaaccgcc cgccggcctc gaaactccac agctcgtcgc
    14581 cctggtagcg ccgccacggc tgcggcccgc cgggggcgga gaacggctgc ccaggcgcgc
    14641 cggtcgtgtt gatccccccg gcgcggtagc cctccgcggc ggtgaggtac cacaggcgcg
    14701 tcgacgatgg acgccaggcc agcaggatct tgggcgccag tccggtctgg cggctggaac
    14761 cctgatagat cgagacggcg cccggggacg ccgcgatccg gctggaagtc cgccccgcca
    14821 cattgaacag ccgcccgccg agggtcagct cgagccggtc ggccagcggc agcgtgacct
    14881 ctccgaaggc ggcgatctca tcgagccggt cgcgccggtt ctcgttcagg gcgggcgggc
    14941 caccggcccc cggccgagcg agcgagaggg tcacgtcctg gcgggcgcga gcgtagaaca
    15001 ggcccgccag ccagcccaca cgggcctcgg cgggcgatag cagcgtggct tcggtcacga
    15061 cactgacgat gccgttctcg tcgtcgaacg ccgccgggcc gggcggcatc ggaaccggcg
    15121 gcgcgatcgt ggcgtcgtaa cggctgtcga gccggtgatc gacgaaggcc gtcgaccagt
    15181 tcagtcgcgc ccagcccaga tcgcccctga gactggcgtt gatctcggcg aagtcgttgt
    15241 cgtgcggctc gcgcaggctg ttgcgccgcg tatagggggg ctccccggcc agggcgtact
    15301 gggtgtcggc cgagtcgatc gcctggccga caattccggc ggacaggctc caccggtcgt
    15361 tcagctcgac ggacgccgcc agacgaccgc ccaggcgcac cgtccggttg acgttggtca
    15421 ggcccaggcc ggcgtcgtcg atgtagccac cggcctgctc ccgccaggcc acggcccgca
    15481 ccgcgccccg ccccccaaac agcggcaggt tgaccatccc ctcca
//
LOCUS       DAORNX010000012        14864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3053_length_14864_cov_8.524141, whole genome shotgun sequence.
ACCESSION   DAORNX010000012 DAORNX010000000
VERSION     DAORNX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_14864_cov_8.524141"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLO65_01055"
     CDS             <1..1130
                     /locus_tag="PLO65_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRD26868.1"
                     /translation="YAGVAAEAPRATNTKLYGEDDPVASPGFSDKVALLQEIDAFARA
                     RDPRVVQVMASMAGERRTVEILRADGRLIRDVRPLVRVNVQVTVERDGRRETGVSGAG
                     GRAGFEAWISPDRWQEQVEQALGQALVNLDAVDCPAGEMDVVLGPGWNGVLLHEAVGH
                     GLEGDFNRKGLSAFSGRIGERVAAPGVTVFDDGSIPGRRGSLTVDDEGTPTSRTILIE
                     DGILVGYMHDRMSARLMGLSPTGNARRESYAHMPMPRMTNTGMLGGRDSQAEMIASTK
                     RGIFCAHLGGGQVDITNGKFVFQCTEAWLIEDGRLTTPVKGASLIGDGPSALTRITMI
                     GDDFAMDPGIGVCGKGGQGVPVGVAQPSLKITGLTVGGTAV"
     gene            1258..3336
                     /locus_tag="PLO65_01060"
     CDS             1258..3336
                     /locus_tag="PLO65_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26869.1"
                     /translation="MSQVLLLALLAGSPEIAAASPPIAAAGQEGVISYPPAFFSAAQP
                     TTAFDMITRIPGFVFDQGDSVRGFAGAAGNVLVDGQRPTTKSDDLQSILRRIPASQVE
                     RIDVIRGGAPGIDMQGKTILANVVVKSGDSVTGLIGVANTFVWDGRQAPALRLEGVRR
                     GNGRTLEGSFVIAGFTDDGSGDGPLIGRDPAGVITDRANMTSEGDGEQAVGTAAYETP
                     LAGGKFRVNGRLFFQRFYFGERDVYTTSPAVATDRELEERIEGEVGLRYGRDLGPRTK
                     IETLFIQQLKTNDFNLVFRVAPDEELFSNSRTLGETIGRATLSFVQSDRLSFETGGEA
                     AFNWLESETSYSSNGVPIALPAANVTVEERRGEVFGQATWKPYTTLTLEGGLRIEGSR
                     ISSEGDVTLEKTLVFAKPRLVATWSPNPANQIRARLEREVGQLNFGDFVASSQINSGG
                     GIATGNPDLNPEQAWVAEIAYERRFWTKGSVTLTLRHTELTDVIDRTPEVVLSTCPLL
                     PGGQPDTDSLACTRFDHPGNIGEGSIDAIAVDFQLPLDRLGVTGGLLKGSAIKRDTEV
                     VDPTTFETRSISGRHPQDWDLAFTQDLPAWKINWGVEVYGGWSETYYRFNRIETVKLR
                     TFVAPYIEWKPTPDISWLLQIQNATSRDLQRQQEFYSGPRNLTPLSGREVRQYDNQPL
                     IYIRLRKQFG"
     gene            complement(3444..5351)
                     /locus_tag="PLO65_01065"
     CDS             complement(3444..5351)
                     /locus_tag="PLO65_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRD26870.1"
                     /translation="MNIVIALGILVTLATGIPVLLQMRAHPKGLFILFFAEMWERFSY
                     YGMRGILIFYLTQHFLFKDDYASGLYGSYTSLVYLLPLLGGILADRYIGTRKAIAFGA
                     LLLVAGHGMMAIEGQPAKQTLNYQGAAYEMQVEGRGSDRKVCIQVGQGCHAFGAGENG
                     ALVIKDLPAASPLPASLPQGSYEMKETRDATYVNIFFLAISLIIMGVGFLKPNISTIV
                     GQLYPEGDPRRDPGFTLYYYGINMGAFWASVLCGYLGQTYGWGWGFGLAGAGMLLGYL
                     AFVLGKPLLEGHGEPPNPVSLAKPVVGPLNQEGLIYIGGILGVGLVWMLVQSNAIVGL
                     VLSLSIVASLIAIVLIIAFVCKTWVQRQRMMLAMILIFGAVVFFTLFEQAGTSLNLFA
                     DRNVDLSLISSPFTLDLFGQSFFFGTRDMVLAAGVPDNVRWIDMGITAAQTQSFNAGF
                     ILIFAPVFAAMWLFLGKHNRDPNPVMKFGLGLVQVGLGFMVIVWGAGMANSAYQMPLI
                     MLGVLYLLHTTGELFLSPVGLSEITKLSMPSVVSFMMAVWFLSSSIAQYVGGIIAGAM
                     GTETVGGQVLDPKGALATSLDGFGKLGWAGIGVGVFFILISFLVKKWGHVGVDGTPHA
                     EIDGDRQSMPH"
     gene            5536..5940
                     /locus_tag="PLO65_01070"
     CDS             5536..5940
                     /locus_tag="PLO65_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HRD26871.1"
                     /translation="MTDEISGAPVTVSAVGGRARALFEGHEVADSEKALFLTERGQPP
                     VCYFPREDVQTSVLRPNDHATSDPWLGLATWYTIMRDAKVVEDVAWSYDQPFDDGAAI
                     TGFIAFAAEHVDFEFETPAPVRHVPAHDPPYV"
     gene            complement(5980..7353)
                     /locus_tag="PLO65_01075"
     CDS             complement(5980..7353)
                     /locus_tag="PLO65_01075"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRD26872.1"
                     /translation="MATTVRDDRPLTLDDLTGWFASGSKPASQWRVGAEHEKFGFHLK
                     THAPVQYGGPDGIEAMLRGLMRFGWEGVYEDRADGGRTLIALERNGASVSLEPGGQFE
                     LSGAPLSSMHEICEETGSHLQEVKQVADELGLGFLGLGFHPTLRREDAAIMPKGRYVI
                     MRRYMPKVGGMGLDMMFRTCTVQANLDFSSEADMAAKFRTSLALQPIATALFANSPFT
                     EGKPNGFLSARANVWTDTDPDRTGMLDFVFEDGFGFERYARYALDVPMYFVKRKGLYI
                     DVAGRSFRDFIDGKLDEVPGDRATLKDWVDHTSTIFPEVRLKTYLEMRGADGGPWSRL
                     CALPALWTGIFYDGAALAAAWDLCKAWTPEDREAVRRDVNRLGLKATVAGRSVQDVAR
                     DMIAISKEGLKRRARLDGGFLDETTYLGELEAIADSGLTPAERLLEKFHGPWQGDISR
                     VFEECAY"
     gene            complement(7481..8215)
                     /locus_tag="PLO65_01080"
     CDS             complement(7481..8215)
                     /locus_tag="PLO65_01080"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRD26873.1"
                     /translation="MIRLHVPDDLAAGADVTPSPDQGRYLTTVMRLAVGAEVLLFNGR
                     DGEWRATLVEAGKRTCRLAVAARTRPQVTGPDLELVVALVKRARLETIIEKAAELGAR
                     RVRLAVTRRTNADHTNVARLQAIAVEAAEQTGRLDVPEIVPPEKLDRLLDGWDPSRRL
                     MFCDEGGGVPPALLALSTPRLGGEDCRKGAILIGPEGGFAPEEGERLRALPFVTAVSL
                     GPRILRADTAAIAALTLWQAALGDWR"
     gene            8261..9019
                     /gene="ppk2"
                     /locus_tag="PLO65_01085"
     CDS             8261..9019
                     /gene="ppk2"
                     /locus_tag="PLO65_01085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRD26874.1"
                     /translation="MDNDAYDAELRTLQIAMIGAQQLAMKDGRKILTIFEGRDAAGKD
                     GTIARVIAPLSRRETRVVALPKPSDREQTQWYFQRYVAHLPAAGEFVLFNRSWYNRAG
                     VERVMGFSTPDQQEEFLREVPNFERMLVASGTRVLKFWLDISADTQAKRLKARRTDPL
                     KVFKTSPLDEVAQEKWDDYTAARDEMLKRTDSDQAPWTCVRADHKKAARLNVLRWILH
                     ATLPEKLCKTVAKPDPSVIFRFEPEAVADGRLHR"
     gene            complement(9016..9903)
                     /locus_tag="PLO65_01090"
     CDS             complement(9016..9903)
                     /locus_tag="PLO65_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRD26875.1"
                     /translation="MTALTSSDVPETQRGGAAADRAFRLHCLNRDSTNGALLKYRDWQ
                     GYMVTGQHSGTHWIKWMLSHAIAAQYGVEPPRYFNNASSNEIIGHPKHRRVHGDLPRI
                     ASSHSIPPYALDRRWVRALLPVPPYGVVVRNIRDVMISNYEKWRGTDRKTYDVPFSRY
                     VAGDPTGKAYVCDVWWYVHFLNRWGEVASRFPAETLVIRYEDFQADRRAALERLARHL
                     GLTLSAAALDAGAAAGAKDVMAGHQDPNIDEKPLRMDGVKAATFSDADEAMLRGILDR
                     NLRHDFGYGYLGAPRGFQA"
     gene            9999..10982
                     /locus_tag="PLO65_01095"
     CDS             9999..10982
                     /locus_tag="PLO65_01095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD26876.1"
                     /translation="MEYVRLGKTGLKVSRLCLGCMTYGSKSWRPWVLEEEDARPFFRA
                     AIEGGINFFDTANMYSLGQSEVVTGKLLKEFAAGRDEVVIATKVNFPMGPGPNDRGLS
                     RKHIMSAIDASLTRLGVDHVDLFQIHRFDYDTPVEETLEALHDVVKAGKARYIGASSM
                     YAWQFAKMLSVSAQNGWTKFVSMQPQYNLVYREEEREMLPLCKAEGIGVIPWSPLARG
                     FLAGGRSKVGEGNTERARTDEFAPRLYYQDADFAVVDAVEAIAGARGLSNMQVALAWV
                     LRNPAVTAPIIGASKLNHLTEALSALDVALGGDEVATLEAPYRPKPVLDHQ"
     gene            11089..>11876
                     /locus_tag="PLO65_01100"
     CDS             11089..>11876
                     /locus_tag="PLO65_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRD26877.1"
                     /translation="MTPKFDLSLKQARRIALTAQGFAESRRHPAPGKRHFGKVAGRLG
                     VIQIDSVNVLTRTHYLPAFSRLGDYPQAVLEAEAWGRKRTLFEYWGHEASLLPLEMQP
                     LLRWRMARAEAGETWTGLARFGRERRDYIDGILDEIEKRGPVTGGDFATGPRGQPGWW
                     SWSEGKRALEWLFWTGYLTTATRRGFERVYDLTERALPAAIIDLPTPTEADAQRELVR
                     RSARAMGVDTEADLRDYYRLPLDGARRAVSELVEAGELTPVKVQG"
     assembly_gap    11877..12110
                     /estimated_length=234
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12111..12300
                     /locus_tag="PLO65_01105"
     CDS             <12111..12300
                     /locus_tag="PLO65_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD26878.1"
                     /translation="RQAGALLVQAAHAEPEAGSDTPAALAAELARMAAWLGLERVEAA
                     AKGDLAGRLQAALGAPAI"
     gene            12410..13120
                     /locus_tag="PLO65_01110"
     CDS             12410..13120
                     /locus_tag="PLO65_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRD26879.1"
                     /translation="MRELDERLSRRGLIVGLAALTASCATSTPKSSLVAQAGPGPISV
                     SPQPLAVPPPPPVALGPVLDPDYLIRRPLMHAALDALKRHGDRIERQDRIYIVDFQAH
                     SSRPRLFRLDLVTGKATAFRTAHGRGSDPAHTGYAQRFSNVPESNASSIGAYLTAGQA
                     WGSKHGPNVLLDGLDPTNSEVRDRAIIVHAADYCEPAYLASQGKLGRSFGCFAVSQAD
                     LNALRPDMDGGRLLFAGA"
     gene            complement(13211..14713)
                     /locus_tag="PLO65_01115"
     CDS             complement(13211..14713)
                     /locus_tag="PLO65_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HRD26880.1"
                     /translation="MRVLTVLGLAAALAFGGFSAASAADKPADAAAGKAPDADLPPFP
                     ADAKVKQVTHVAGKTLSYTVTVGSLPVRDDKGKVIAEVVFTSYVLDGPRNPNRPVTFA
                     FNGGPGAASVYLNLGAIGPKRVQFGAQGDAPSDSAALQDNPYTWLDFTDLVFIDPVGT
                     GFSRSRTTPEESKKAFFGVKQDIAYLSRIVYDWLLKNERMTSPKYITGESYGGFRGPR
                     LTHYLQTELGVGINGVVLVSPFFDGTSWSGGDFSPMPWAVTLPSMAAAHYERQGKALS
                     PEQMADVEHYVRTEFLTDFMAGRTNQPALERIIKRVTAYTGLRESVVRQMGGRIDSRA
                     FLRELYRDEGRIGSWYDSNVTAWDPYPWAPNQRSGDPILDALIAPTTSAMVDFVTRVV
                     GWKIEARYNALSDAVNEAWEDELPTIEAVSAFRKALANDPKMKVLIVHGYDDLSCPYF
                     ASRIVIDQLPPMGNPGRVQLKVYPGGHMFYSRPDSGAQMRQDVMAMYGAG"
BASE COUNT         2360 a         4970 c         5057 g         2243 t
ORIGIN      
        1 gctacgccgg agtcgccgca gaggcgccgc gcgcgacaaa cacgaaactc tatggcgagg
       61 acgacccggt cgcctcgccc ggcttctccg acaaggtcgc cctgctgcag gagatcgacg
      121 ccttcgcccg cgcccgcgat ccccgtgtgg tccaggtgat ggcctcgatg gccggcgagc
      181 ggcgcacggt cgagatcctg cgcgccgacg gccggctgat tcgcgatgtt cgcccgctgg
      241 tgcgggtcaa cgtccaggtt acggtcgagc gcgatggccg ccgcgagacg ggcgtgtccg
      301 gcgccggcgg ccgggccggc ttcgaggcct ggatctcgcc cgacaggtgg caggaacagg
      361 tcgagcaggc cctgggccag gcgctggtca acctggacgc cgtcgactgt ccggccggcg
      421 agatggacgt ggtgctcggc ccgggctgga acggcgtgct gctgcacgag gccgttggtc
      481 acggcctgga aggcgacttc aaccgcaagg gcctttcggc cttctccggc cgcatcggcg
      541 agcgcgtcgc cgcgccgggc gtcacggtgt tcgacgacgg ctcgattccg ggccgccgcg
      601 gttcgctgac cgttgatgac gaaggcaccc cgacctcgcg gacgatcctg atcgaggacg
      661 gcatccttgt cggctacatg cacgaccgca tgagcgcccg gctgatgggc ctcagcccca
      721 ccggcaacgc ccggcgcgag tcctacgccc acatgccgat gccgcggatg accaacaccg
      781 gcatgctcgg cggcagggac agccaggccg agatgatcgc ctcgacgaaa cggggcatct
      841 tctgcgccca cctcggcggc ggccaggtcg acatcaccaa cggcaagttc gtgttccagt
      901 gcaccgaggc ctggctgatc gaggacggca ggctgaccac gccggtcaag ggcgccagtc
      961 tgatcggcga cggccccagc gccctgaccc gcatcaccat gatcggcgac gacttcgcca
     1021 tggacccggg catcggcgtc tgcggcaagg gcggccaggg cgtgccggtc ggcgtggccc
     1081 agccgtcgct gaagatcacc gggctgacgg tcggcgggac agcggtctga cggctcacgc
     1141 ctacgccaat cctgacaccg aattaatcca catttcaggc ttgtaacgtg ccccggcggc
     1201 gctcgccggt caccttcgcc ccagttcagg gccggggggc catatggggg acgccaaatg
     1261 agccaggtcc tgttgctcgc gcttctcgcc ggttcgccgg agatcgccgc cgcatcgccg
     1321 ccgatcgccg cggcggggca ggagggcgtg atctcctatc cgccggcctt cttctccgcc
     1381 gcccagccga caaccgcctt cgacatgatc acccgcattc cggggttcgt gttcgaccag
     1441 ggcgacagcg tgcgcggctt cgccggcgcg gccggcaacg tgctggtcga cggccagcgc
     1501 ccgaccacca agagcgacga cctgcagagc atcctgcggc ggattccggc cagccaggtc
     1561 gagcggatcg atgtcatccg cgggggcgcg cccggcatcg acatgcaggg caagaccatc
     1621 ctcgccaatg tggtggtcaa gtccggcgat tcggtcaccg gcctgatcgg cgtggccaac
     1681 accttcgtct gggatggtcg ccaggccccg gccctgcggc ttgagggcgt gcggcgcggc
     1741 aatggccgga ccctcgaggg ctcgttcgtc atcgccggct tcaccgacga tggctccggc
     1801 gacggtccgc tgatcggccg cgacccggcc ggggtcatca ccgaccgggc caacatgacg
     1861 tccgagggcg atggcgagca ggccgtgggc accgccgcct acgagacgcc gctggccggt
     1921 ggcaagttcc gcgtcaacgg ccggctgttc ttccagcgct tctatttcgg cgagcgcgac
     1981 gtctacacca ccagtccggc cgtcgcgacc gaccgggagc tcgaggagcg gatcgagggc
     2041 gaggtcggcc tgcgctacgg tcgcgacctg ggcccgcgga ccaagatcga gaccctgttc
     2101 atccagcaac tgaagaccaa cgacttcaac ctggtgttcc gcgtcgcgcc cgacgaggag
     2161 ctgttttcca acagccgcac gctcggcgag accatcggcc gggcgacgct gagttttgtc
     2221 cagtcggacc ggctgtcgtt cgagaccggc ggcgaggccg ccttcaactg gctcgagagc
     2281 gagaccagct acagctcgaa cggcgtcccg atcgccctcc cggccgccaa cgtcaccgtc
     2341 gaggagcgcc gtggcgaagt gttcggccag gcgacgtgga agccctatac gaccctgacc
     2401 ctcgagggcg gcctgcgcat cgaggggtcg cgcatctcgt ccgagggcga cgtcacgctg
     2461 gagaagaccc tcgtcttcgc caagccgcgg ctggtggcga cctggtcgcc caatccggcc
     2521 aaccagatcc gcgcgcgcct ggagcgcgag gtcggccagc tgaacttcgg cgacttcgtc
     2581 gccagttcgc agatcaattc cggcggcggc atcgccacgg gcaatcccga tctcaacccg
     2641 gagcaggcct gggtggcgga aatcgcctat gagcgccggt tctggaccaa ggggtcggtg
     2701 accctgacgc tgcgccatac cgaactgacc gacgtgatcg accgcacgcc cgaagtggtg
     2761 ctgtcgacct gtccgctgct gccgggcggc cagcccgaca ccgattcgct ggcctgcacc
     2821 cggttcgatc atcccgggaa catcggcgag ggctcgatcg acgccatcgc cgtcgacttc
     2881 cagctgccgc tcgaccgcct tggggtcacc ggcggccttc tgaagggcag cgcgatcaag
     2941 cgcgacaccg aggtggtcga tccgacgacc ttcgagacgc gctcgatttc cggtcgccat
     3001 ccgcaggact gggacctggc cttcacccag gacctgccgg cctggaagat caactggggc
     3061 gtcgaggtct atggcggctg gagcgagacc tactatcgct tcaaccggat cgagacggtc
     3121 aagctgcgca ccttcgtggc gccctacatc gagtggaagc cgacgccgga catcagctgg
     3181 ctgctgcaga tccagaacgc cacctcgcgc gacctgcaac gccagcagga gttctattcc
     3241 ggcccgcgca acctgacgcc gctgagtggc cgcgaggtgc gccagtacga caaccagccg
     3301 ctgatctaca tccgcctgcg caagcagttc ggctgacgct cggcactcgc cagccgtctt
     3361 ccgagcccct gggaggcggc cgacgaagaa gggccccggg atcgctcccg gggcccttcg
     3421 ccgtttcagt gaggatcagg cgatcagtgc ggcatcgact gccggtcgcc atcgatctca
     3481 gcgtgcggcg tcccgtcgac gccgacatgg ccccacttct tcaccaggaa gctgatcagg
     3541 atgaagaaca cgcccacgcc gatgccggcc cagccgagct tgccgaagcc gtcgagcgag
     3601 gtggccaggg cgcccttcgg gtcgagcacc tgtccgccga ccgtctcggt gcccatggcg
     3661 ccggcgatga tgccgccgac gtactgggcg atcgaactgg acaggaacca cacggccatc
     3721 atgaacgaca ccaccgaggg catcgacagc ttggtgattt ccgacaggcc gaccggcgac
     3781 aggaacagct cgccggtggt gtgcagcagg tacagcaccc ccagcatgat cagcggcatc
     3841 tggtaggccg agttggccat tcccgcgccc cagacgatga ccatgaagcc gaggccgacc
     3901 tgcaccaggc ccaggccgaa cttcatcacc gggttggggt cgcggttgtg cttgcccagg
     3961 aacagccaca tcgccgcgaa caccggggcg aagatcagga tgaagccggc gttgaacgac
     4021 tgggtctggg cggcggtgat gcccatgtcg atccagcgca cgttgtcggg cacgccggcg
     4081 gccagcacca tgtcacgggt gccgaagaag aagctctggc cgaacaggtc cagggtgaag
     4141 ggcgaactga tcagcgacag gtcgacgttg cggtcggcga acaggttgag cgaggtgccc
     4201 gcctgctcga acagggtgaa gaacaccacg gcgccgaaga tcaggatcat cgccagcatc
     4261 atgcgctggc gctggaccca ggtcttgcag acgaaggcga tgatcaggac gatggcgatc
     4321 agcgaggcga cgatgctgag gctcagcacc aggccgacga tggcgttgct ctgcaccagc
     4381 atccagacca ggcccacgcc gaggatgccg ccgatataga tcaggccttc ctggttcagc
     4441 gggccgacga cgggcttggc caggctcacc ggattgggcg gctcgccgtg gccctcgagc
     4501 agcggcttgc cgagcacgaa ggccaggtag ccgagcagca tgccggcgcc ggccagaccg
     4561 aagccccagc cccagccata ggtctggccg agatagccgc acagcaccga ggcccagaag
     4621 gcgcccatgt tgatgccgta gtagtagagg gtgaagcccg ggtcgcggcg cggatcgcct
     4681 tccggataga gctggccgac gatcgtggag atgttcggct tcaggaagcc gacgcccatg
     4741 atgatcagcg agatcgccag gaagaagatg ttcacgtagg tcgcgtcgcg cgtctccttc
     4801 atctcgtagg agccctgcgg caggctggcg ggcagcgggg aggcggccgg cagatccttg
     4861 atcaccagtg cgccgttctc gcccgcgccg aaggcgtggc agccctggcc gacctggatg
     4921 cagaccttgc gatcggagcc gcggccctcg acctgcattt cgtaggccgc gccctggtag
     4981 ttgagggtct gcttcgccgg ctggccctcg atggccatca tgccatggcc ggccaccagc
     5041 agcagcgcgc cgaaggcaat ggccttgcgc gtgccgatgt accggtcggc caggatgccg
     5101 ccgagcaggg gcagcaggta gaccagcgag gtgtaggacc cgtacaggcc gctcgcgtag
     5161 tcgtctttga acaggaaatg ttgcgtgagg tagaagatga ggatgccgcg catcccgtag
     5221 taggagaacc gctcccacat ctccgcgaag aagaggatga agaggccctt cgggtgcgcc
     5281 cgcatctgga gcagcaccgg gatgcccgtg gccagggtca cgaggatccc cagcgcgata
     5341 acgatattca tactgcctac cctgtgactc tatggccccg cgcggacgga agacggcaag
     5401 attccccccg aaccgccgcg atttgagcgc gagaaaatca tgcgctggcc ggtttcacaa
     5461 gcaaggcctt gccgcgcgcg tcattcggga gccgacctgt ggccgccgcg ttatccctgc
     5521 caggaggacg ccgccatgac ggacgagatt tcaggcgccc cggtcacggt cagcgccgtg
     5581 ggcggccggg cgcgggccct gttcgagggc cacgaggtcg ccgacagcga gaaggccctg
     5641 tttctgaccg aacggggcca gcctcccgtc tgctattttc cgcgcgagga cgtgcagacg
     5701 tcggtgttgc ggcccaacga tcatgccacc agcgatccct ggctcggcct ggcgacctgg
     5761 tacacgatca tgcgcgacgc caaggtcgtc gaggacgtgg cctggtccta cgaccagccg
     5821 ttcgacgacg gcgcggcgat caccggcttc atcgctttcg cggctgaaca tgtggacttc
     5881 gagttcgaga cgcccgcgcc ggtgcgccat gtcccggcgc atgatccgcc atacgtctga
     5941 acccattcac tctccccatg gaaagggagg gaggaagtcc tagtacgcgc actcctcgaa
     6001 cacgcggctg atgtcgccct gccaggggcc gtggaacttc tcaagcagcc gttccgccgg
     6061 ggtcagtcca ctgtcggcga tcgcctcgag ctcgcccaga taggtggtct cgtcgaggaa
     6121 gcccccgtcg agccgggcgc ggcgcttgag gccttccttg ctgatggcga tcatgtccct
     6181 ggccacgtcc tgcaccgagc ggccggcgac cgtcgccttc agacccagac gattgacgtc
     6241 gcgtcgaacg gcctcccggt cttccggagt ccaggccttg cagaggtccc aggccgcggc
     6301 cagggcggcc ccgtcgtaga agatgccggt ccacagggcg ggcagggcgc acagccggct
     6361 ccacggaccg ccgtccgcgc cacgcatctc cagataggtc ttcagccgca cttccgggaa
     6421 gatggtgctg gtgtggtcga cccagtcctt gagcgtcgcg cggtcgccgg gaacctcgtc
     6481 cagttttcca tcgatgaagt cgcggaacga ccggccggcc acgtcgatgt agaggccctt
     6541 gcgcttgacg aaatacatcg gcacgtcgag ggcgtagcgc gcatagcgct cgaagccgaa
     6601 gccgtcctcg aagacgaagt ccagcatgcc cgtgcggtcg ggatcggtgt cggtccagac
     6661 gttggcgcgg gccgacagga agccgttggg cttgccctcg gtgaacgggg aattggcgaa
     6721 cagggcggtg gcgatcggct gcagcgccag gctggtgcgg aacttggccg ccatgtcggc
     6781 ttcggagctg aagtcgagat tggcctggac cgtgcaggtg cggaacatca tgtccaggcc
     6841 catgccgccg accttgggca tatagcgccg catgatcacg tagcgcccct tgggcatgat
     6901 cgcggcgtcc tcgcgcctca gggtcggatg gaagccaagg ccgagaaagc cgagtcccag
     6961 ttcatcggcc acctgcttga cctcctgcag gtggctgccc gtctcctcgc agatctcgtg
     7021 catcgagctc agcggcgcgc cgctgagctc gaactggccg cccggctcga ggctgacgct
     7081 ggcgccgttg cgttccagcg cgatcagggt ccggccgccg tcggcgcgat cctcgtagac
     7141 gccttcccag ccaaatcgca tcagacccct gagcatcgcc tcgatgccgt cgggcccgcc
     7201 atactgcacc ggcgcgtggg ttttcagatg aaagccgaac ttttcatgct ccgccccgac
     7261 ccgccattgg ctggcgggct tgctgccgga ggcgaaccag ccggtcaggt cgtcgagtgt
     7321 caggggcctg tcgtcacgga cggtcgtggc catatcgctg tgcctcccca ggcattttgc
     7381 agtggttagg gaggcccctt ccccgaccgg gcgcgatcaa tacgctcaag tgcaggtggg
     7441 agacaatcac gcttggcgca aggcttcgtg acgggcgttg ttatcgccag tcgcccagcg
     7501 ccgcctgcca gagcgtcagg gcggcgatgg ccgcggtgtc ggcgcgcagg attcttggac
     7561 cgagggacac ggcggtgacg aagggcaggg cccgcaggcg ctcgccttcc tcaggtgcga
     7621 atccgccttc cggaccgatc aggatggcgc ccttccgaca atcctccccg cccaggcggg
     7681 gagtgctgag cgcaagcagc gccggtggga ccccgccgcc ctcgtcgcag aacatcagcc
     7741 ggcgcgatgg atcccagccg tcgagcaggc ggtcgagctt ttccggcggc acaatctcgg
     7801 ggacgtccag ccgtccggtc tgttccgcgg cttccacggc gatggcctgc agccgcgcga
     7861 cattggtgtg gtcggcgttg gtccgccgcg tcacggccag gcgcacgcgc cgggcgccga
     7921 gctcggcggc cttttcgatg atcgtctcca gccgcgcccg cttgaccagc gccacgacca
     7981 gttcaaggtc ggggcccgtg acctgtggcc gggtccgcgc ggcgacggcc agccggcagg
     8041 tccgcttgcc ggcctcgacc agcgtcgccc gccactcgcc gtcgcggccg ttgaacagca
     8101 gcacctccgc acccacggcc agccgcatga ccgtggtcag gtagcggccc tggtcgggcg
     8161 acggggtcac atcggcgccg gcggcgaggt catcgggaac gtgaagacgg atcatcggac
     8221 tcttctagcc gccggaccgg gtccggcggt agggtcgggc atggacaatg atgcatatga
     8281 cgcggaactg cggaccctgc agatcgccat gatcggggcc cagcagctgg ccatgaagga
     8341 tggccggaaa atcctgacga tcttcgaggg ccgtgacgcg gccggcaagg acggcacgat
     8401 cgcccgggtc atcgcgcccc tgtcgcgccg cgaaaccagg gtcgtcgccc tgcccaagcc
     8461 gtccgaccgg gaacagacgc aatggtactt ccagcgctac gtcgcccacc tgccggcggc
     8521 cggtgagttc gtgcttttca accgctcctg gtacaaccgg gcgggcgtcg agcgcgtcat
     8581 ggggttcagc acgccggacc agcaggagga gtttctgcgc gaggtgccga acttcgaacg
     8641 gatgctggtc gccagcggaa cgcgcgtgct caagttctgg ctcgacattt ccgccgacac
     8701 ccaggccaag cgcctcaagg cccggcgcac cgacccgctg aaggtgttca agaccagccc
     8761 gctggacgag gtggcccagg agaaatggga cgactacacg gcggcgcgcg acgagatgct
     8821 caagcgcacc gacagcgacc aggcgccgtg gacctgcgtc cgcgcggacc acaagaaggc
     8881 ggcccggctg aacgtgctgc gctggattct gcacgccacc ctgccggaga agctgtgcaa
     8941 gaccgtggcg aagcccgacc catcggtgat cttccgcttc gagcccgaag ccgtcgccga
     9001 tggccggctg caccgctagg cctggaagcc tcgcggcgcg ccgaggtagc catagccgaa
     9061 gtcgtgcctg aggttgcgat cgaggatgcc gcgcagcatg gcctcgtcgg cgtccgagaa
     9121 cgtcgccgcc ttcacgccgt ccatgcgcag gggcttctcg tcgatgttcg ggtcctggtg
     9181 gcccgccatg acgtccttgg cccccgccgc cgcgcccgcg tccagcgccg cggccgacag
     9241 ggtcaggcca aggtgcctgg ccagccgctc cagagccgcg cggcgatccg cctggaagtc
     9301 ctcgtagcgg atcaccaggg tctcggccgg gaaacggctg gccacctcgc cccagcggtt
     9361 gaggaagtgg acgtaccacc agacgtcgca gacataggcc ttgccggtcg gatcgcccgc
     9421 cacatagcgc gaaaagggca cgtcgtaggt cttgcggtcg gtcccccgcc acttctcgta
     9481 attcgagatc atcacgtcgc ggatgttgcg caccaccacg ccatagggcg gaaccggcag
     9541 cagcgcccgc acccacctgc ggtcgagggc gtagggcggg atcgagtgcg agctggcgat
     9601 gcggggcagg tcgccgtgca cccgcctgtg cttcggatgg ccgatgatct cgttggagga
     9661 cgcgttgttg aagtagcgcg gcggctcgac gccatactgg gcggcgatgg cgtggctcag
     9721 catccacttg atccagtggg tccccgaatg ctggccggtg accatgtagc cctgccagtc
     9781 gcggtatttc agcagggcgc cgttggtgct gtcgcggttg aggcagtgca gccggaaggc
     9841 ccggtcggcg gcggccccgc cgcgctgggt ttccggaacg tcgctgcttg tcaaagccgt
     9901 caaggtcgtc actctcgctg ccgccacgtt cgggccggaa cgggggctat atgcgacagg
     9961 aagcggcggc gtgtcacgac ggccgcgagg agagcacgat ggaatacgtc cgcctcggca
    10021 agaccggcct caaggtctcc cgcctctgcc tgggctgcat gacctatggc agcaagtcct
    10081 ggcggccgtg ggtgctggag gaggaggacg cccggccgtt cttccgcgcg gccatcgagg
    10141 gcgggatcaa cttcttcgac acggccaaca tgtactcgct gggccagagc gaagtggtca
    10201 ccggcaagct gctcaaggaa ttcgcggccg gccgcgacga ggtggtcatc gccaccaagg
    10261 tcaacttccc gatgggaccc ggcccgaacg acaggggcct gtcgcgcaag cacatcatga
    10321 gcgcgatcga cgccagcctg acgcggctgg gcgtcgacca tgtcgacctg ttccagatcc
    10381 accgcttcga ctacgacacg ccggtcgagg agacgctcga ggcgctgcat gacgtggtga
    10441 aggccggcaa ggcccgctac atcggggcct cgtcgatgta cgcctggcag ttcgcgaaga
    10501 tgctcagcgt gagcgcgcag aacggctgga cgaagttcgt ctccatgcag cctcagtaca
    10561 acctcgtcta tcgcgaggaa gagcgggaga tgctgccgct gtgcaaggcc gaggggatag
    10621 gcgtgattcc ctggtcgccg ctggcgcgcg gctttctggc cggcggccgc agcaaggtcg
    10681 gcgagggcaa taccgaacgg gcccgcaccg atgagttcgc cccgcgtctc tactatcagg
    10741 acgccgactt cgccgtggtc gacgcggtcg aggccatcgc cggcgcgcgg gggctctcga
    10801 acatgcaggt cgccctggcc tgggtgctgc gcaatccggc ggttacggcc ccgatcatcg
    10861 gcgcctcgaa gctgaaccac ctgaccgagg ccctgtcggc gctggacgtc gcgctcggcg
    10921 gcgacgaggt cgcgacgctg gaggcgccct acaggcccaa gccggtgctg gatcaccaat
    10981 gatggcgtgc gtccttcgac acggccctgc gggcctgctc aggatgacgt gcggtagcgc
    11041 cccacgtcat ggtgagcagc gctcgaagag cgcgtgtcga accacgcaat gaccccgaaa
    11101 ttcgacctct ccctcaagca ggcccgccgc atcgccctga cggcccaggg cttcgccgag
    11161 tcgcgccgcc acccggcgcc gggcaagcgc cacttcggca aggtcgccgg gcggctgggc
    11221 gtgatccaga tcgacagcgt caatgtgctg acccgcaccc actacctgcc ggccttctcg
    11281 cggctgggcg actatccgca ggcggtgctg gaggccgagg catggggcag gaagcgcacc
    11341 ctgttcgaat actggggcca cgaggcgtcg ctgctgccgc tggagatgca gccgttgctg
    11401 cgctggcgca tggcccgggc cgaggccggc gagacctgga cgggcctcgc ccgtttcggc
    11461 cgcgagcggc gggactacat cgacggcatc ctggacgaga tcgagaagcg cggcccggtg
    11521 accggcggcg acttcgccac gggcccgcgt ggccagcccg gctggtggag ctggtccgag
    11581 ggcaagcgcg cgctcgagtg gctgttctgg accggctacc tgaccacggc gacccgccgc
    11641 gggttcgagc gggtctacga cctgacggaa cgggcgctgc cggcggcgat catcgacctg
    11701 cccacgccga ccgaggccga cgcgcagcgg gagctggtgc ggcgctcggc ccgggccatg
    11761 ggcgtggaca ccgaggcgga cctgcgcgac tactatcgcc tgccgctcga cggcgcccgc
    11821 cgggcggtga gcgagctggt cgaggccggc gagctgacgc cggtgaaggt ccagggnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggcaggcc
    12121 ggggcgctgc ttgtgcaggc cgcgcacgcc gagccggaag ccggatcgga cacgccggcc
    12181 gccctcgccg cggagctcgc gcgcatggcc gcctggctag ggctggagcg ggtcgaggct
    12241 gccgcgaaag gcgatctggc cggccgtttg caggccgcgc tcggggcgcc cgcgatctga
    12301 ccactgtcgg gaaagtgagc gggtgcgaca caaaacccgg ttcgcatccg acggtcggtg
    12361 ggctatcgag ccggcttgcc gcgcgggggc gccaagcgga gtagccatct tgagagagct
    12421 ggacgaacgt ctttcccgac ggggcctgat cgtcggcctg gcggccctga ccgcatcctg
    12481 cgccacctcg acgccgaagt cgtcgctggt cgcacaagct ggccccggac cgatttccgt
    12541 gtcgccccag ccgctcgccg ttccgccgcc gccccccgtc gccctcggcc cggtgctcga
    12601 tcctgactat ctgatccgcc gtccgctgat gcacgcggcg ctggacgcgc tcaagcgcca
    12661 cggcgaccgg atcgagcgcc aggaccgcat ctacatcgtc gacttccagg cccattcgag
    12721 ccgtccgcgc ctgttccggc tggatctggt caccggcaag gcgaccgcct tccgtacggc
    12781 gcacggccgc ggctccgatc cggcccacac cggctacgcc cagcgctttt ccaatgtccc
    12841 ggagtcgaac gcctcgtcga ttggcgccta tctgaccgcg ggccaggcct ggggctccaa
    12901 gcatgggccc aatgtcctgc tggacgggct ggacccgacc aattccgagg tccgcgaccg
    12961 ggcgatcatc gtccacgcgg cggactactg cgagccggcc tacctggctt cgcagggcaa
    13021 gctgggccgc tcgttcggct gtttcgcggt ctcccaggcg gacctcaacg ccctccgccc
    13081 cgacatggac ggcggccgcc tgctgttcgc gggggcgtga cgacagccag ggacatgcag
    13141 ttcagtgcgt gtccctcgcc gcttcgccgc tgacggggac acgcactgaa gtgcatgtcc
    13201 tccttgtgcc tcaccccgcc ccgtacatcg ccatcacgtc ctgccgcatc tgcgcgccgc
    13261 tgtcggggcg gctgtagaac atgtgcccgc ccggatagac cttcagctgc acccggcccg
    13321 gattgcccat gggcggcagc tggtcgatga cgatgcgcga ggcgaagtag gggcagctga
    13381 gatcgtcgta gccgtggacg atcagcacct tcatcttcgg atcgttggcc agcgccttgc
    13441 ggaaggccga caccgcctcg atggtcggca gctcgtcctc ccaggcctcg ttcaccgcgt
    13501 cgctgagggc gttgtagcgg gcctcgatct tccagccgac gacgcgggtg acgaagtcga
    13561 ccatggcgct ggtcgtgggc gcgatcaggg cgtcgaggat cgggtcgccg ctgcgctggt
    13621 tcggcgccca ggggtagggg tcccaggccg tgacgttgga atcgtaccag ctgccgatgc
    13681 gcccctcgtc gcggtagagc tcgcgcagga aggcgcggct gtcgatccgc ccgcccatct
    13741 ggcgcaccac cgactcgcgc aggccggtgt aggcggtgac ccgcttgatg atccgctcta
    13801 gcgccggctg gttggtccgc ccggccatga agtcggtcag gaattcggtg cggacatagt
    13861 gttcgacgtc ggccatctgc tccggcgaca gcgccttgcc ctggcgctcg tagtgggccg
    13921 cggccatcga gggcagggtc acggcccagg gcatcggcga gaagtcgccg cccgaccagc
    13981 tggtgccgtc gaagaacggc gagaccagca ccacgccgtt gatgccgacg cccagttcgg
    14041 tctgcagata atgggtcagg cgcgggccgc ggaagccgcc gtagctttcg ccggtgatgt
    14101 atttcggcga ggtcatccgc tcgttcttga gcagccagtc atagacgatg cgcgacaggt
    14161 aggcgatgtc ctgcttgacc ccgaagaagg ccttcttgct ctcctccggc gtggtccgcg
    14221 agcgcgagaa accggtgccg accgggtcga tgaacaccag gtcggtgaag tccagccagg
    14281 tgtaggggtt gtcctgcaac gcggccgagt cggacggtgc gtcgccctgg gcgccgaact
    14341 gcacgcgctt ggggccgatg gcgccgaggt tgagatagac gctggccgcg cccgggccgc
    14401 cgttgaaggc gaaggtcacg ggccggttcg gattgcgcgg gccgtccagc acgtaggagg
    14461 tgaacaccac ctcggcgatg accttgccct tgtcgtcgcg caccggcagc gagccgaccg
    14521 tgaccgtgta gctcagcgtc ttgccggcga cgtgggtgac ctgcttgacc ttggcgtcgg
    14581 cggggaaggg cggcaggtcg gcgtcggggg ccttgccggc ggcggcgtca gcgggcttgt
    14641 cagcggcgga ggcggcgctg aagccgccga acgccagggc ggccgccagg ccgagaaccg
    14701 tcagaacacg catggtgact ccccccaaaa cagatacgcg gcggaagtag tatcgggcgg
    14761 aacgagggtg gggaagtgtt gcgacgatcc tccccgcgaa gccggggagg gggaccacgc
    14821 ggagcatggt ggagggtctg cgccggactg tccggagtga ccgg
//
LOCUS       DAORNX010000013        14549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3138_length_14549_cov_6.469229, whole genome shotgun sequence.
ACCESSION   DAORNX010000013 DAORNX010000000
VERSION     DAORNX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_14549_cov_6.469229"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..77
                     /locus_tag="PLO65_01120"
     CDS             <1..77
                     /locus_tag="PLO65_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26881.1"
                     /translation="NETTRERRPDLWEAHLANRQPKGS"
     gene            105..506
                     /gene="rplS"
                     /locus_tag="PLO65_01125"
     CDS             105..506
                     /gene="rplS"
                     /locus_tag="PLO65_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRD26882.1"
                     /translation="MAANIIKELEKAESERLAALRTTPDFQPGDTVRVNVKIKEGERE
                     RVQAYEGVCIARQGAGLHESFTVRKISFGEGVERVFPIMSPMIESIEVKRRGVVRRAK
                     LYYLRDRRGKSARIAERQMARKEDAPATGEE"
     gene            687..1067
                     /locus_tag="PLO65_01130"
     CDS             687..1067
                     /locus_tag="PLO65_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HRD26883.1"
                     /translation="MSRSPAETVRQFLTAMERLDYNAGLVLVAEDCAYINPPPLGAVQ
                     GPAGIRAVLEPFFAPTLENDFQILREAAAGPVVILERLDRHRLADKWVELPVTGVFEV
                     RDGRITYWRDYFDAATILSQWPAG"
     assembly_gap    1068..1167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1314..2105)
                     /locus_tag="PLO65_01135"
     CDS             complement(1314..2105)
                     /locus_tag="PLO65_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD26884.1"
                     /translation="MTAETVTYEVADHVATITLNRPEQRNALNWDAYAKLEQYLRKAV
                     TDPEARCVIVTGADPAFCSGDDVREIMAGPKAVSADTTPVVRHRPTPAAMAALECDKP
                     MIAAVNGAAIGWGMELALYADIRIASEQAKFSEMFIKRGLVCDVGGFYRLPSIVGPAK
                     AAELLFTGDVITAAEALRIGLVSEVTPHAELLPRARALAARIAVNPPLALRYMKEGLR
                     RGTYGDPRELGGWAIETIRALMKTEDHKEGVASFLEKREPVFKGW"
     gene            complement(2353..4158)
                     /locus_tag="PLO65_01140"
     CDS             complement(2353..4158)
                     /locus_tag="PLO65_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRD26885.1"
                     /translation="MRRRTFVSALALALPGSAIAASAVFAREGVQDQALTAFLDAAFD
                     ETTALSPQSMTSLGIKTDYDRLDDYTDAGAQKRLAVAERQLAEMKAKFRPGELGPAGQ
                     LSYRLFERQVEQQRESFRWRLHGYIASVNGSPAGSIPVFLINQHRVDSVEDAWAYIAR
                     LRDVKRVMAEVSVALQTRAEAGIRAPAFTFAPVEADTRKVVTGAPFTDGPDSALWADF
                     GKKVAALAAPDADKAALLAGARAALTGPFREGYDQFLATQASIAKDATTSNGAWALPD
                     GAAYYANRLKVQTTTSQTADEIHQTGLDELARIQGEMRAIMAKVGFTGSLQAFFAELK
                     TNPKFQYPNTPEGKAAYLRDATGLIAQVMKIAPQWFLRLPKAALEVRAVEPWREATAS
                     IAFYNRPSPDGKRPGIYYVNLSDMTQVLKPQIEGISYHEGAPGHHFQIALAMETAGLP
                     KFRRFGGYGAYSEGWGLYAERLGKEMGFYQDPYSDFGRLSTEAWRAVRLVTDTGLHAK
                     RWTREQAIAFFRENTLLSERDIVKEVERYLCNPGQATSYKTGELKIMALRSKAKAALG
                     AKFDIRQFHDAVLRDGALPLDVLGEQVEAWMATQG"
     gene            4341..5765
                     /gene="leuC"
                     /locus_tag="PLO65_01145"
     CDS             4341..5765
                     /gene="leuC"
                     /locus_tag="PLO65_01145"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRD26886.1"
                     /translation="MAGKTLYDKIWDAHRVADDAGETILYIDLHLIHEVTTPQAFAGL
                     RAEGRKVRRPDRTLAVADHNVPTEGQALGVGAVADEEARLQLQTLARNVADNGIEFFP
                     MGDIRNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAQGIGTSEVEHVLASQT
                     LRQQKSKNMRVTVNGKLSAGVTAKDMALAIIGRIGTAGGTGHVIEFAGEAVRDLSMEG
                     RMTLCNLTIEGGARAGLVAPDDKTFAYLQGRPAAPKGAAWDMAVSYWKTLFTDPDAHF
                     DREVVLDGPSIAPTVTWGTSPEDVVPITGNVPDPSAYDTPDKKAAVERALDYMGLTAG
                     QPITQARIDRVFIGSCTNSRIEDLRAAAAVAQQAFLEGRLVADHVRAMVVPGSGLVKE
                     QAEEEGLDAIFRAAGFDWREPGCSMCLGMNPDRLAPGERCASTSNRNFEGRQGRGGRT
                     HLMSPAMAAAAAIAGHIVDVRDFL"
     gene            5762..6607
                     /locus_tag="PLO65_01150"
     CDS             5762..6607
                     /locus_tag="PLO65_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD26887.1"
                     /translation="MTINALDHVALVVSDLAAAEAGYSRILGVTPNWSGTMHGASHVW
                     FQLGNTALDVIAPTGDGPVGDSIRARIAGHGEGLWALSFSTPDLAAERKRLDRLGIPT
                     YERAEVSTNPDTGAARSWTTAAMRTKSTHGANLILVENRPDAAPWPPAETGPAPVTGL
                     DHVVVNTPDPERAAALYGARLGLAMKLDRSNPDWGTRLMFFRLGDLVVEVAHSLKAGV
                     SDGPDAPWGLSWRVADVAAAHARMVAAGSDVSDVRVGRKPGTAVFTVKDAPGGVPTLV
                     IGPAG"
     gene            6604..7215
                     /gene="leuD"
                     /locus_tag="PLO65_01155"
     CDS             6604..7215
                     /gene="leuD"
                     /locus_tag="PLO65_01155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD26888.1"
                     /translation="MKPFTRLDARAAPLPLANIDTDQIIPKQFLKTVDREGLAKGLFY
                     DLRFDEQGREKSGFVLNDPAYRGAQILIAGDNFGCGSSREHAPWALQDFGIQCVISTG
                     FADIFRGNCLNNGLLTVELKPEEVQALMDEAKGGNHLFTVDLETQTVTAPSGATFAFE
                     IDADRKHKLLKGLDAIGETLQHVGAIDLFESRAALNRPWLERT"
     gene            7212..7511
                     /locus_tag="PLO65_01160"
     CDS             7212..7511
                     /locus_tag="PLO65_01160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRD26889.1"
                     /translation="MILVIGTVRLPPANILKAHPAMKTMIAASRAEDGCLDYAYSGDL
                     VEPGLIRVTEVWRDREALAAHFQTPHMAAWRAVFAELGITERRLALYEAGEPEPI"
     gene            7519..8574
                     /gene="leuB"
                     /locus_tag="PLO65_01165"
     CDS             7519..8574
                     /gene="leuB"
                     /locus_tag="PLO65_01165"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRD26890.1"
                     /translation="MSQSLLLLPGDGIGPEVTAQVRRVAAALTPDLRLDERDFGGVSY
                     DRHGVPLTDEATATALASDAVLMGAVGGPQWAGVPRDKRPEMGLLGLRKAMDVYANLR
                     PALCFDALADASSLKRELVAGLDIMFVRELTGGVYFGQPRFIETLPDGQRRGVDTQVY
                     TTGEIERVSRVAFELARGRRNKVHSLEKSNVMETGLLWREVVTDLHRREYADVQLEHM
                     LADNAAMQLVRQPTQFDVLLTDNLFGDVLSDEAAQLTGSLGMLPSAALGAAGRPGLYE
                     PIHGSAPDIAGQGVANPLAAILSFEMALRWSLNRQAEADRLFAAVEAALAAGARTRDL
                     GGALSTVEMGDAVLRAL"
     gene            8962..9681
                     /locus_tag="PLO65_01170"
     CDS             8962..9681
                     /locus_tag="PLO65_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HRD26891.1"
                     /translation="MTPADADHEARARAELAAWRGAMLAEPSAFARAAKGLQTQVNRM
                     IPEKIHAAVTKVIEQMTRGILAGSDFVSGPPLAAGSLREREARVRKAIDGYRNVATLE
                     GGATGAGGFLLAAADFPALIAIKIKLLFEIAGLYGHDMRDWRERLYVLSIFQLAFSSA
                     DHRRDVLLAMAEGRQPASPEDFDWRRFQQEYRDYIDLAKLAQLLPVVGAPIGAIVNFR
                     LVEQLGETAMNAYRMRWFEGR"
     gene            complement(9678..10352)
                     /locus_tag="PLO65_01175"
     CDS             complement(9678..10352)
                     /locus_tag="PLO65_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD26892.1"
                     /translation="MKVGPDIALVAALLGDPARANMLTALLAGQALTAGELAREAGVT
                     PQTASAHLSRLEKGGLIVGRKQGRHSYFALSGDDVAGVLEGLMSLAARTGHARTRPGP
                     REPALRRARVCYDHLAGELGVAMLDSLVASGRLDADLQPTVYGEAFFAALGVDLAALK
                     AGRRPLCRACLDWSVRRSHLAGSLGAAVLNRLYALGWAKRLEGERVVAFTEAGERKFA
                     EVFGLP"
     gene            10432..10740
                     /locus_tag="PLO65_01180"
     CDS             10432..10740
                     /locus_tag="PLO65_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRD26893.1"
                     /translation="MAVVCFIRYVIDPYQRDAFEAYARNWLTIIPACGGELIGYFLPH
                     EGTDNIAQALIGFDSLAAYEAYRARLKSDPAGAANFRLARDGRFILSEERTFLRPVTA
                     "
     gene            10737..11081
                     /locus_tag="PLO65_01185"
     CDS             10737..11081
                     /locus_tag="PLO65_01185"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRD26894.1"
                     /translation="MIAVIFEVIPRGGLRDDYLATAARLAPLLDGIDGFLSIERFESL
                     TRPGKILSLSFWRDEAAVAAWRGLSEHRTAQADGRARIFADYRLRIAGVVRDYGLFDR
                     DQAPEDSRVALR"
     gene            complement(11224..11613)
                     /locus_tag="PLO65_01190"
     CDS             complement(11224..11613)
                     /locus_tag="PLO65_01190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD26895.1"
                     /translation="MAFDLSALPFARLMGVEITTADKDRVEGRLTVRDDLCTAGGILH
                     GGAMMAFADSLGAVGAFLNLAPGARTTTLESKTNFIGSARVGTTVTAVSTPLHVGRRS
                     SVWQTRLEGEDGRLVALITQTQMTLEG"
     gene            complement(11676..12671)
                     /locus_tag="PLO65_01195"
     CDS             complement(11676..12671)
                     /locus_tag="PLO65_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD26896.1"
                     /translation="MKTLRLGAAGPAVSALGLGCMGMSTDWYGPSDRAESLATFQAAV
                     DAGITLIDTGDFYGMGHNEMLVGEALRSVDRDKVVLSVKMGGLRDPAGGFLGQDCRPA
                     AVKTFLAYSLKRLQVDYIDIYRPARLDPAVPIEDTVGAIADMIKAGYVRHVGLSEVGA
                     DTIRRAAATHPIADLQIEYAIITRGIEDRILPTCRELGIGVTAYGVLARGLISGHWSK
                     HRSVAGDLRGFNPRFQGENLDHNLALVEALRAIAAARGVTMAQLAIAWVAQRGDDIIP
                     LVGARRRDRLGEALGALDVTLTAAELAAIEAAAPRGAAAGERYPPMMLQHMDSER"
     gene            12764..13669
                     /locus_tag="PLO65_01200"
     CDS             12764..13669
                     /locus_tag="PLO65_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD26897.1"
                     /translation="MPAPDIADLDAFAAVARARSFRGAAALRNVSPSSLSQAVKRLEE
                     RLGVRLLNRTTRSVTPTEAGQRLMTRLAPTLAELTQALDEVNSFRDSPTGTLRLNVPS
                     VAARLVLPPLVTEFLAAHPGISLELVAEDSFVDVLAGGFDAGVRYDERLEKDMIAIPL
                     GPPERYVTVGSPAYFDRRSRPAHPDELLGHLCIRHRFLSGVSMPWEFEKDGQVVRIVP
                     RGPLVSNVADLGIMAAEAGLGLVRTFEGFARTRIDSGRLETVLDDWSPPFPGPNLYYP
                     SRRHMPAPLRAFVDFLQARRRMTNR"
     gene            13723..13920
                     /locus_tag="PLO65_01205"
     CDS             13723..13920
                     /locus_tag="PLO65_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26898.1"
                     /translation="MTEPTPQDPPPPPAFSGPAGVALIGAGVGLMVVGQTVLHMPAMA
                     YAGLAILAVGIVFGVMSQKGK"
     gene            13920..14465
                     /locus_tag="PLO65_01210"
     CDS             13920..14465
                     /locus_tag="PLO65_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26899.1"
                     /translation="MRPPAMLIASLLLTAAPAAAQSPPPAPAASPPACATPEYRQFDF
                     WVGRWDVYPTGSDKLVAHSLIERLYGGCAIRENWMPLTGGGGGSLSNYVAGEKGWRQT
                     WLDSTGARVEFTGGRIGEAMVITGSWPGAVTPGQAALVRMTYTAAADGSVRQFGEAST
                     DGGKTWATNFDFTYRPAKATP"
BASE COUNT         2146 a         5172 c         4972 g         2159 t
ORIGIN      
        1 gtaacgagac gacccgcgag cggcggcccg acctctggga agcccacctc gccaatcgac
       61 aaccaaaggg cagttgaagc ccgacgaacc gaaggaagac cacgatggcc gcgaacatca
      121 tcaaggaact ggagaaggcc gagagcgaac gcctcgccgc cctccgcacc acccccgatt
      181 tccagccggg cgacaccgtc cgggtgaacg tgaagatcaa ggaaggcgag cgcgagcgcg
      241 ttcaggccta cgaaggcgtc tgcatcgccc gccagggcgc cggcctgcac gagagcttca
      301 cggtccgcaa gatcagcttc ggcgaaggcg ttgagcgggt gttcccgatc atgtcgccga
      361 tgatcgaaag catcgaagtg aagcgtcgcg gcgtcgtccg tcgcgccaag ctctattacc
      421 tgcgcgatcg tcgcggcaag tcggcccgca tcgccgagcg ccagatggcc cgcaaggaag
      481 acgccccggc caccggcgag gaatagtcct cctctcgcgc ccgccgaacg gaacgagccc
      541 cggcccgcat ggtccggggc tttttcgcgc ccggggacat gtaccgaagg gacgtgtccc
      601 cttcaggtag ccgcccacga cctggcaggt aattgagccg gcggccgcgc cgcccgagat
      661 tgcgtccatc gcccagagga gacgccatgt cccggtcccc cgccgaaacc gtccgccagt
      721 tcctgacagc catggagcgg ctcgactaca acgccggcct cgtgctcgtg gccgaggatt
      781 gcgcctacat caacccgccg ccgctgggcg cggtccaggg tccggcgggc atccgcgccg
      841 tgctggagcc gttcttcgcc ccgacgctcg agaacgactt ccagatcctg cgggaagccg
      901 cggcgggccc ggtggtcatc ctcgaacggc tcgatcgcca ccgcctggcc gacaaatggg
      961 tcgaactgcc ggtgaccggg gtgttcgagg tgcgtgacgg ccggatcacc tactggcgcg
     1021 actacttcga cgcggcgacg atcctgtccc agtggccggc cgggtgannn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnctg gcgcgactac ttcgacgcgg cgacgatcct
     1201 gtcccagtgg ccggccgggt gagcgagccg gggacatgga ccgaaggtcc gtgtccccca
     1261 agcgagctgc gcgggtttag gggacacgga ccttcggtcc atgtcctcgc ctgtcaccat
     1321 cccttgaaca ccggctcgcg cttttccagg aagctggcca cgccctcctt gtgatcctcg
     1381 gtcttcatca gggcgcggat ggtctcgatg gcccagccgc cgagctcgcg cgggtcgccg
     1441 taggtcccgc ggcgcaggcc ctccttcatg tagcgcaggg ccagcggcgg attgaccgcg
     1501 atcctcgccg ccagcgccct ggcgcgcggc agcagctcgg cgtgcggcgt cacctcgctg
     1561 accaggccga tgcgcagggc ctccgctgcg gtgatcacgt cgccggtgaa cagcagctcg
     1621 gcggccttgg ccgggccgac gatcgacggc aggcgataga agccgcccac gtcgcagacc
     1681 aggccgcgct tgatgaacat ctcgctgaac ttcgcctgct ccgaggcgat gcggatgtcg
     1741 gcgtagaggg ccagctccat gccccagccg atggccgcgc cgttgaccgc cgcgatcatc
     1801 ggcttgtcgc attcgagcgc cgccatggcc gcgggggtgg gcctgtggcg caccaccggc
     1861 gtcgtgtcgg ccgaaaccgc cttgggcccg gccatgatct cgcgcacgtc gtcgccggag
     1921 cagaaggcgg ggtccgcgcc ggtgacgatc acgcagcggg cctcggggtc ggtgaccgcc
     1981 ttgcgcaggt actgttccag cttcgcatag gcgtcccagt tcagcgcatt gcgctgctcg
     2041 ggccggttca gggtaatggt cgcgacatgg tcggcgacct cgtaggtgac ggtctcggcg
     2101 gtcatcggcg gctcctgtcc aggccgatga tgcggcgggc gggagggggc ttcaatggcg
     2161 ccgtggcgtc actccaatct ctatcctccc cgcgaagccg gggaggggga ccgcccgaag
     2221 ggcggtggag ggggcagcac gaacgcgaac ggcctcatcc tgacgtccgg ggagtccggg
     2281 gagtccggcg ctgacccctc caccatgctc cgcgtggtcc ccctccccag cgcgcttcgc
     2341 ttgtggggag aattatcctt gagtggccat ccacgcctcc acctgctcgc ccagcacgtc
     2401 gagtggcaag gccccgtcac gcagcacggc gtcgtggaac tgacggatgt cgaacttcgc
     2461 gcccagcgcc gccttcgcct tgctccgcag ggccatgatc ttcagctcgc cggtcttgta
     2521 gctggtcgcc tggcccggat tgcacaggta gcgctcgacc tccttcacga tgtcccgctc
     2581 gctgagcagg gtgttctcgc ggaagaaggc gatggcctgt tcgcgcgtcc accgcttggc
     2641 atgcaggccg gtgtcggtga ccaggcgcac ggcgcgccag gcctcggtcg agagccggcc
     2701 gaagtccgag tatgggtcct ggtagaagcc catctccttg cccagccgct cggcgtagag
     2761 gccccagccc tccgaatagg cgccgtagcc gccgaagcgg cggaacttcg gcaggccggc
     2821 ggtctccatg gccagggcga tctggaagtg atggcccggc gccccctcgt gatagctgat
     2881 gccctcgatc tggggcttca gaacctgggt catgtccgac aggttgacgt agtagatgcc
     2941 cggccgtttc ccgtccggcg agggccggtt gtagaaggcg atcgaggcgg tggcctcacg
     3001 ccagggctcg accgccctca cctccagcgc ggccttcggc aggcgcagga accactgcgg
     3061 ggcgatcttc atcacctggg cgatcagtcc ggtggcgtcc ctcaggtagg ccgccttgcc
     3121 ctccggcgtg ttggggtact ggaacttcgg attggtcttc agctcggcga agaaggcctg
     3181 cagcgagccg gtgaagccga ccttcgccat gatcgcgcgc atctcgccct ggatgcgcgc
     3241 cagctcgtcc aggccggtct ggtggatctc gtcggcggtc tggctggtgg tggtctgcac
     3301 cttgaggcga ttggcgtagt aggccgcccc gtcaggcagg gcccaggcgc cgttactggt
     3361 ggtcgcatcc ttcgcgatcg aggcctgggt ggcgaggaac tggtcgtacc cttcgcggaa
     3421 cggaccggtc agggcggcgc gggctccggc gagcagggcg gccttgtcgg cgtccggcgc
     3481 cgcaagggcg gcgaccttct tcccgaaatc cgcccacagg gcgctgtcgg gaccgtcggt
     3541 gaacggcgcg ccggtgacca ccttgcgggt gtcggcctcg accggcgcga aggtgaaggc
     3601 cggggcgcga atgccggcct cggcgcgggt ctgcagggcg acgctgactt cggccatgac
     3661 ccgcttcaca tcccgcaggc gggcaatgta ggcccaggcg tcctcgacgc tgtcgacccg
     3721 gtgctggttg atcaggaaca ccgggatcga gccggcgggg ctgccgttga cgctggcgat
     3781 gtagccgtgc agccgccagc ggaaactctc gcgctgctgc tcgacctggc gctcgaacag
     3841 gcgatacgac agctggccgg ccgggcccag ctcgccgggg cggaacttcg ccttcatctc
     3901 cgcgagctgc cgttcggcca cggccagccg cttctgggcc cccgcgtcgg tatagtcgtc
     3961 gagcctgtcg tagtcggtct ttatccccag gctggtcatc gactgggggc tcagcgccgt
     4021 ggtctcgtcg aaggccgcgt cgaggaaggc ggtcagggcc tggtcctgca ccccctcgcg
     4081 ggcgaagacg gccgaggcgg cgatggccga gcccggcagg gcgagcgcca gggcgctgac
     4141 aaaggttcgt cttctcacgg cggcgactcc tgcggatcca tcgccgccag caccgcacga
     4201 tttggcgcgg ctgtcaccgt ggtcatcccc gctccccgcc cgtcaccccg ggccctgtgt
     4261 cgagccacgc cggcaagatc atgcccgttc gtcgcgcgcc ggactggacg aaggggcaag
     4321 cctcgcccta catgccgccc atggctggca agacgctcta cgacaagatc tgggacgctc
     4381 accgggtggc ggacgacgcc ggcgagacga tcctctacat cgatctgcat ctgatccacg
     4441 aggtgaccac gccgcaggcc ttcgccgggc tgcgcgccga gggccgcaag gtgcgccggc
     4501 ccgaccgcac cctggcggtg gccgaccaca atgtcccgac cgagggccag gccctgggcg
     4561 tgggggcggt ggccgacgag gaggcccggc tgcagctgca gaccctggcc cgcaatgtcg
     4621 ccgacaacgg catcgaattc ttcccgatgg gcgacatccg caacggtatc gtccatgtgg
     4681 tcgggcccga gcagggccgg acccagccgg gcatgaccat cgtctgcggc gattcccaca
     4741 cctcgacgca cggcgccttc ggggccctgg cccagggcat cggcaccagc gaggtcgagc
     4801 acgtgctggc cagccagacc ctgcgccagc agaagtcgaa gaacatgcgc gtgacggtga
     4861 acggcaagct gtcggccggc gtcaccgcca aggacatggc cctggccatc atcggcagga
     4921 tcggcacggc cggcggcacc ggccatgtca tcgagttcgc cggcgaggcc gtgcgcgacc
     4981 tctccatgga gggccgcatg accctctgca acctgaccat cgagggcggc gcccgcgcgg
     5041 gtctggtcgc gccggacgac aagaccttcg cctatctgca gggccggccg gccgccccca
     5101 agggcgcggc ctgggacatg gccgtgtcgt actggaagac cctgttcacc gatccggacg
     5161 cgcacttcga ccgcgaggtg gtgctggacg ggccgtcgat cgcgccgacc gtgacctggg
     5221 gcaccagtcc cgaggacgtc gtgccgatca ccggcaacgt ccccgacccg tcggcctacg
     5281 acaccccgga caagaaggcc gccgtcgagc gggcgctgga ctacatgggc ctgaccgccg
     5341 gccagccgat cacgcaggcg cgcatcgacc gggtgttcat cggcagctgc accaacagcc
     5401 ggatcgagga cctccgcgcc gccgccgccg tggcgcagca ggccttcctc gagggccggc
     5461 tggtcgccga ccatgtcagg gcgatggtcg tgccgggctc ggggctggtg aaggaacagg
     5521 ccgaggagga ggggctggac gccatcttca gggccgccgg ctttgactgg cgcgagcctg
     5581 gctgctcgat gtgcctgggc atgaaccccg accgcctggc gccgggcgag cgctgcgcct
     5641 cgaccagcaa ccgcaacttc gagggtcgcc agggtcgcgg cggccgcacc catctgatga
     5701 gcccggcgat ggccgcggcg gcggcgatcg cgggtcacat cgtcgatgtg agggacttcc
     5761 tgtgacgatc aacgcgctcg accacgtcgc cctggtggtg agcgacctcg ccgccgccga
     5821 ggccggctac agccgcattc tgggcgtgac acccaactgg agcgggacca tgcacggggc
     5881 cagccacgtc tggttccagc tcggcaacac cgccctcgac gtcatcgccc cgacggggga
     5941 cggcccggtc ggcgacagta tccgcgcccg tatcgccggc catggcgagg gcctctgggc
     6001 cctgtcattc agcacgccgg acctggccgc cgaacgcaaa cggctcgatc gccttggcat
     6061 cccgacctac gagcgggccg aggtctcgac caacccggac accggcgcgg cgcgcagctg
     6121 gacgacggcg gcgatgcgca cgaagtcgac gcacggcgcc aacctgatcc tcgtcgagaa
     6181 ccggcccgac gccgcgccct ggcccccggc cgagaccggc ccggcgccgg tcaccgggct
     6241 cgaccatgtg gtggtcaaca cgcccgaccc cgagcgcgcc gcggccctct atggcgcgcg
     6301 gctgggcctg gccatgaagc tcgaccgctc gaaccccgac tggggcacgc ggctgatgtt
     6361 cttccgtctc ggcgacctgg tggtcgaggt ggcgcacagc ttgaaggccg gggtctcgga
     6421 cggcccggac gcgccctggg gcctgtcctg gcgcgtcgcc gatgtcgccg ccgcccacgc
     6481 ccgtatggtc gccgccgggt ccgatgtctc cgacgtccgc gtcggccgca agcccggcac
     6541 cgccgtcttc accgtcaagg acgcccccgg cggcgtgccg accctcgtga ttggacccgc
     6601 tggatgaaac cgttcacccg cctcgacgcc cgcgccgcgc cgctgccgct ggccaacatc
     6661 gacaccgacc agatcatccc caagcagttc ctcaagacgg tcgaccgtga gggcctggcc
     6721 aaggggctgt tctacgacct gcggttcgac gagcaggggc gcgagaagag cggcttcgtg
     6781 ctcaacgacc cggcctaccg gggcgcgcag atcctcatcg ccggcgacaa cttcggctgc
     6841 ggcagctcgc gcgagcacgc cccctgggcg ctgcaggact tcggcatcca gtgcgtcatc
     6901 tcgaccggct tcgccgacat cttccgcggc aactgcctga acaacggcct gctgacggtc
     6961 gagctgaagc ccgaggaggt ccaggcgctg atggacgagg ccaagggcgg caaccacctg
     7021 ttcacggtcg acctcgagac ccagaccgtc acggccccct ccggcgcgac ctttgcgttc
     7081 gagatcgacg cggaccgcaa gcacaagctg ctcaagggcc tggacgccat cggcgagacc
     7141 ctgcagcacg tcggcgcgat cgacctgttt gaatcgaggg ccgccctgaa ccggccctgg
     7201 ctggagcgga catgatcctg gttatcggca cggtccggct gccgccggcg aacattctca
     7261 aggcccaccc ggcgatgaag accatgatcg ccgcgagccg ggccgaggac ggctgcctcg
     7321 actacgccta ttcaggcgat ctcgtggagc ccggcctgat ccgcgtcacc gaggtctggc
     7381 gcgaccgcga ggccctggcg gcccatttcc agaccccgca catggccgcc tggcgcgccg
     7441 tgttcgccga gctgggcatc accgaacgca ggctggccct ctacgaggcc ggcgagcccg
     7501 aaccgatcta gagagtccat gtcccagtcc ctgctcctgc tgcccggcga cgggatcggc
     7561 cccgaagtca cggcccaggt gcgccgcgtg gccgccgccc tgaccccgga cctgaggctc
     7621 gacgaacgtg acttcggcgg cgtcagctac gaccgccacg gcgttcccct gaccgacgag
     7681 gcaaccgcca cggccctggc cagcgacgcg gtgctgatgg gcgccgtggg cggaccgcag
     7741 tgggccggcg ttccgcgcga caagcggccg gagatgggcc tgctgggcct gcgcaaggcc
     7801 atggacgtct acgccaacct gcgcccggcc ctctgtttcg atgccctggc cgacgcctcg
     7861 agcctgaagc gggagctggt cgccgggctc gacatcatgt tcgtccgtga gctgacgggc
     7921 ggcgtctatt tcggccagcc tcggttcatc gagaccctgc ccgacggcca gcgccgcggc
     7981 gtcgacaccc aggtctacac caccggcgag atcgagcggg tcagccgggt ggccttcgaa
     8041 ctggcgcgcg gtcgccgcaa caaggtccat tcgctcgaga agtcgaacgt gatggagacc
     8101 ggcctgctgt ggcgcgaggt ggtcaccgac ctgcaccggc gcgaatacgc cgacgtccag
     8161 ctcgagcaca tgctggccga caacgcggcc atgcagctgg tccgccagcc gacccagttc
     8221 gacgtgctgc tgaccgacaa cctgttcggc gacgtgctgt cggacgaggc cgcgcagctg
     8281 accgggtcgc tgggcatgct gccgtcggcg gcgctgggcg cggcgggcag gcccggcctg
     8341 tacgagccga tccacggttc ggcccccgac atcgccggcc agggcgtggc caatccgctg
     8401 gcggcgatcc tcagcttcga gatggccctg cggtggtcgc tgaaccgtca ggccgaggcc
     8461 gaccggctgt ttgccgccgt cgaggcggcg ctggccgccg gcgcccgcac gcgcgacctc
     8521 ggcggagcgc tctcgaccgt cgagatgggc gacgcggtgc tgcgggcttt gtgaggcctg
     8581 cctttgccag gatgtagact agccctttgg ccttgatgga atgatgcgct ccggtcggtc
     8641 gccctggtca aggacggccc tgcgggccgc cgctgcgcgg ccgccgaagg cggtccttga
     8701 gcaggccgtc cgccggagca acactggcca tcaagacatc agggccggtc tgccaaatgc
     8761 ccttcctctc ccggagggag aggtggcccg gcagcgccgg gtcggagagg gaggaaccac
     8821 cggtccgcgc ggttcgcgtc cctctctgcc accatctgcg cctccctctc caccatgctc
     8881 cgcatggtcc ccctctccct ccgggagagg attgtcatga gccgggatga ctctggcgtg
     8941 tcccgatcct aagtctcctc catgaccccc gccgacgccg accacgaagc ccgcgcccgc
     9001 gccgaactcg ccgcctggcg cggggccatg ctggccgaac cctcggcctt cgcgagggcg
     9061 gccaagggcc tgcagaccca ggtcaaccgg atgatcccgg agaagatcca tgcggccgtg
     9121 accaaggtca tcgagcagat gacccgcggc atcctggccg gctcggactt cgtcagcggc
     9181 ccgccgctcg ccgccggctc cttgcgggag cgggaggcgc gggtgcgcaa ggcgatcgac
     9241 ggctaccgca atgtcgccac cctcgagggc ggcgcgaccg gcgccggcgg cttcctgctg
     9301 gcggcggcag acttcccggc cctgatcgcc atcaagatca agctgctgtt cgagatcgcc
     9361 ggcctgtacg gtcacgacat gcgcgactgg cgcgaacggc tgtacgtcct gtcgatcttc
     9421 cagctggcct tctccagcgc cgaccatcgc cgggacgtgc tgctggcgat ggccgaaggc
     9481 cgtcagcccg cctcgcccga ggacttcgac tggcggcgat tccagcagga atatcgcgac
     9541 tacatcgacc tggcgaagct ggcccagctg ttgccggtcg tcggcgcgcc gatcggggcg
     9601 atcgtcaatt tccgcctggt cgaacagctc ggcgagacgg ccatgaacgc ctaccggatg
     9661 cggtggttcg aaggccgcta gggcagcccg aacacctccg cgaacttcct ctcccccgcc
     9721 tccgtgaacg ccacaacccg ctcgccctcc agccgtttcg cccagcccag ggcatagagc
     9781 cggttcagca cggccgcgcc cagggacccg gccaggtggc tgcggcgcac gctccagtcg
     9841 aggcaggccc tgcacagcgg ccggcggccg gccttcagcg cggcgagatc gacacccagg
     9901 gcggcgaaaa aggcctcgcc atagaccgtg ggctgaagat cggcgtcgag tcgaccggac
     9961 gcgaccaggc tgtcgagcat cgccaccccc agctctcccg ccagatggtc gtagcagacc
    10021 cgcgcgcggc gcagggcggg ctccctcggg ccggggcggg tgcgggcatg gccggtgcgg
    10081 gcggccaggc tcatcagtcc ctccagtacg ccggccacgt catcgcccga cagggcgaag
    10141 tagctgtgcc ggccctgttt gcggccgacg atcagcccgc ccttctcgag ccgcgacaga
    10201 tgggcgctgg ccgtctgggg cgtgacgccg gcctcccggg ccagctcgcc ggcggtcagg
    10261 gcctggcccg ccagcagggc ggtcagcatg ttggcccggg cggggtcgcc gagcagggcg
    10321 gcgaccaggg cgatgtcggg gcctaccttc atgcttcgat cgtgcgcgaa acatcgccgc
    10381 cgggcaagcg actacatttc gctgcacatc gaaccctgtc ggagcccttg catggccgtc
    10441 gtctgcttca tccgctacgt catcgatccc taccagcgcg acgccttcga ggcctatgcg
    10501 cgcaactggc tgacgatcat tcccgcctgc ggcggcgagc tgatcggcta tttcctgccg
    10561 cacgagggga ccgacaacat cgcccaggcc ctgatcggct tcgacagcct ggcggcctac
    10621 gaggcctatc gcgcccggct gaaatccgat ccggccgggg cggccaactt ccgcctggcg
    10681 cgggacgggc ggttcatcct cagcgaggag cggaccttcc tgcggccggt gacggcatga
    10741 tcgcggtgat cttcgaggtc atcccgcgcg gaggcctgcg cgacgactat ctcgccaccg
    10801 cggcccggct ggccccgctg ctggacggga tcgacggctt cctgtcgatc gagcggttcg
    10861 agagcctgac ccggccgggc aagatcctgt cgctgtcgtt ctggcgcgac gaggcggccg
    10921 tggcggcctg gcggggactc tccgagcacc ggacggcgca ggcggacggc cgtgcacgga
    10981 tcttcgcgga ctatcgcctg cgcatcgccg gggtggtgcg ggactacggc ctgttcgacc
    11041 gggaccaggc gccggaggat tcacgggtcg cccttcgata gcagcggcgg agtcgccctg
    11101 atgtctcgat gagcattctt gctccggcgg tccttgagca agggcacgtc cggagcgaac
    11161 cattccatca aggcctccag ggtgtatcgg catccaggga cgtgggcgga accgggacga
    11221 ccctcacccc tccagcgtca tctgggtctg cgtgatcagg gccaccagcc gcccgtcctc
    11281 gccctccagc cgggtctgcc agacgctcga gcggcggccg acgtgcaggg gcgtcgagac
    11341 cgcggtcacg gtggtcccca ccctggccga gccgatgaag ttggtcttgc tctcaagcgt
    11401 cgtagtccgc gcgcccggcg cgaggttgag gaaggcgccg acggccccca ggctgtcggc
    11461 gaaggccatc atcgccccgc cgtgcaggat gccgccggcc gtgcagagat cgtcgcggac
    11521 cgtcagccgg ccctcgaccc ggtccttgtc ggcggtggtg atctccaccc ccatcagccg
    11581 ggcgaacggc agggcggaca ggtcgaaggc catggcggat gatccctgga ggacggtggg
    11641 gtccaccctg gcagggatcg cgggcgcgga cgatcctatc gttcgctgtc catgtgctgc
    11701 agcatcatcg gcggatagcg ctcgcccgcc gccgcgccgc gcggggcggc cgcctcgatg
    11761 gccgccagct cggctgcggt cagggtcacg tccagggcgc ccagcgcttc gcccaggcga
    11821 tcccgccgac gggcgccgac caggggtatg atgtcgtcgc cccgctgggc gacccaggcg
    11881 atggcgagct gggccatggt cacgcccctg gccgccgcga tggcgcgcag cgcctccacc
    11941 agcgccagat tgtggtcgag gttctcgccc tggaagcggg gattgaagcc gcgcaggtca
    12001 ccggccaccg accggtgctt gctccagtgg ccactgatca ggccgcgcgc gaggacgcca
    12061 taggccgtga cgccgatgcc cagttcgcgg caggtgggca ggatcctgtc ctcgatgccc
    12121 cgggtgatga tcgcgtactc gatctgcaga tcggcgatcg ggtgcgtcgc ggcggcccgg
    12181 cggatggtgt ccgccccgac ctcggacagt ccgacgtgac gcacgtagcc ggccttgatc
    12241 atgtcggcga tggccccgac ggtgtcctcg atgggcacgg ccgggtcgag ccgcgccgga
    12301 cgatagatgt cgatgtagtc gacctgcagg cgcttcagcg aataggcgag gaaggtcttc
    12361 accgccgctg gccggcagtc ctggccgaga aagccgccgg ccggatcacg cagaccgccc
    12421 atcttgacgc tgagcacgac cttgtcgcga tcgacgctcc tgagcgcctc gccgaccagc
    12481 atctcgttgt gacccatgcc gtagaagtcg ccggtgtcga tcagggtgat cccggcatcc
    12541 acggcggcct ggaacgtggc caggctctcg gcccggtcgc tgggtccgta ccagtccgtc
    12601 gacatgccca tgcagccgag gccgagggcg gaaacggcgg ggccggcggc gccgagtcga
    12661 agcgttttca tgaggatctc cgtctgtctc gcggcactat gcgcgggccc ggtctgtgcg
    12721 ataatccatc accatcgaca caggctgttt cgatttctgc acaatgcccg cgcccgacat
    12781 cgctgatctc gacgctttcg ccgccgtcgc gcgggcgcgc agcttccggg gcgccgcggc
    12841 cctgcgcaac gtctcgccat ccagtctcag ccaggcggtc aaacggctgg aggaacggct
    12901 gggcgtgcgg ctgctgaacc gcaccacccg cagtgtcacc cccacggagg ccggccaacg
    12961 gctgatgacg cgcctcgcgc cgaccctggc cgagctgacc caggcgctgg acgaggtgaa
    13021 cagctttcgc gacagcccga cggggaccct gcgtctgaac gtgcccagcg tggcggcgag
    13081 actggtgctg ccgcccctgg tcaccgagtt cctcgccgcc catcccggca tcagtctgga
    13141 gctcgtcgcc gaggacagct tcgtcgatgt cctggccggc ggattcgacg ccggcgtgcg
    13201 ttacgacgaa cggctcgaga aggacatgat cgccatcccg ctgggaccgc ccgagcgcta
    13261 cgtgaccgtc ggctcgcccg cctatttcga ccgccgaagc cgaccggccc accccgatga
    13321 gctgcttggc cacctctgta tccgccaccg gttcctgagc ggcgtatcga tgccgtggga
    13381 attcgaaaag gacggccagg tggtccgcat cgttccgagg gggccgctgg tctccaacgt
    13441 cgccgacctt ggcatcatgg ccgccgaggc gggcctgggg ctggtgcgaa cgttcgaagg
    13501 cttcgcccgg accaggatcg acagcggccg gctggagacg gtgctggacg actggtcgcc
    13561 gccgtttccc gggccgaacc tctactatcc cagccgccgg cacatgccgg cgcctctgcg
    13621 cgcgttcgtc gacttcctgc aggcccgccg ccggatgacc aatcgctaac ttgtcggcct
    13681 ctggccggcg tcgcatcctc gccggatgtg aggaggggcg ccatgactga acccacgccg
    13741 caagatcccc cgccgccgcc cgccttcagc ggcccggccg gcgtcgcgct catcggcgcg
    13801 ggggtcggcc tgatggtcgt ggggcagacc gtgctgcaca tgccggccat ggcctacgcc
    13861 ggactggcca tcctggccgt cggcatcgtc ttcggcgtga tgagccagaa ggggaagtga
    13921 tgcgaccgcc cgccatgctg atcgccagcc ttctgttgac ggcggcgccg gccgccgccc
    13981 agtcgccgcc gcccgcccct gccgcctcgc cacccgcctg cgcgacgccg gagtatcgcc
    14041 agttcgactt ctgggtcggg cgctgggacg tctatccgac cgggtcggac aagctggtcg
    14101 cccacagcct gatcgaacgg ctctatggcg gctgcgccat ccgcgagaac tggatgcccc
    14161 tgacgggcgg cgggggcggc agcctgagca actacgtcgc cggcgagaag ggctggcgcc
    14221 agacctggct cgattccacc ggcgcccgcg tggagttcac cggcggccgg atcggcgagg
    14281 ccatggtcat caccggatcc tggcctggcg ccgtgacccc gggccaggcc gccctggtgc
    14341 ggatgaccta caccgccgcc gccgacggat cggtccgcca gttcggcgag gcctcgaccg
    14401 acggcggcaa gacctgggcg acaaacttcg acttcaccta caggccggcg aaggcgacgc
    14461 cctgaccgtt caggcgatcg ggctgacgac gagaaactgg ggctcttcga cccaggccat
    14521 gaacgcgccc atcaccgtct ggatcaccg
//
LOCUS       DAORNX010000014        14533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3142_length_14533_cov_7.084749, whole genome shotgun sequence.
ACCESSION   DAORNX010000014 DAORNX010000000
VERSION     DAORNX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_14533_cov_7.084749"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1026)
                     /locus_tag="PLO65_01215"
     CDS             complement(46..1026)
                     /locus_tag="PLO65_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRD26900.1"
                     /translation="MTLRMDARIETWELTKCFRIASGEITRTDLVVVTLEDDGVIGRG
                     EGSPFFVYNQTAAGELAAIERVRQAIEEGVGREALGSLLPPGPARNAIDCALWDIEAK
                     RTGGIWSLLDLPAPVFLDALMTIGLDEPAVMAGEAERLSEFPALKIKLNADRIMERMN
                     AIRAVAPRATLVIDANEGWTGDLLREVAPGLADLGVEMIEQPVPRGQDEALAGFQAPI
                     KLCVDESLHTCADLDRVAPFYDVINIKLDKTGGLTEALKLAELGQDRGLELMVGNMMG
                     TSLSMAPTYVIGSLCAYRDLDGPAHYVADRDHGMTFEHGRSHLFTPALWG"
     gene            complement(1076..2428)
                     /locus_tag="PLO65_01220"
     CDS             complement(1076..2428)
                     /locus_tag="PLO65_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRD26901.1"
                     /translation="MSGASSGKGIGAFGAVVTIVGFVVGASIYILPGEIAATTGPGVV
                     LSYAGAAVLAVFSCVVAAQLGSVFPVSGASFVAIGRLVSPAFAFLCVWGMIGAAVVAV
                     ALLGFGFADYVRLWVPGVDRLVLATALIVGLGGLNLIGVRDTIAAQTLMVAFFLVALL
                     LFAAAALTGGELRPLSPFLPNGLGPVISGAIPAYFSFAGFMVIIELSGEVRRPARNIP
                     LALAISFVIVAMVYALVALAVVAQLPWTDLARVEAPVSSAAARVLPNGVVQVIAFSAV
                     AATASSINVMLMGYSRDLLALARSRALPRLLAGASRRTGEPVAAILAITVLSLVALAF
                     GARIVDYATFVVVGLMLLQVGVGLAALVLPWRDRERYLASAFRIPRPVLVFFSTGLIV
                     LSIAFIVIAASGSPGATAAGAAFIAIGAVYYALRRIWLRRQAISLEAELARELGFDHT
                     "
     gene            complement(2425..4302)
                     /locus_tag="PLO65_01225"
     CDS             complement(2425..4302)
                     /locus_tag="PLO65_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRD26902.1"
                     /translation="MTRARALARMLALVVVLAFVPQAAWSQTPALTSQYIEMRDGVRI
                     AVDVWTPPDVGAEGAPTVLLLTRYWRSRVVASPAPEADVNFERARLFAKAGYALAIVD
                     VRGSGASFGSRVTEFSPDEIRDYGEIIAWIAAQPWSNGRVATTGSSYLGNTAELAAIH
                     GGDALRAAVPRFSDFDTYRYAVFPGGVPNTVLLKAWGEIVGALDRDDPCVAAGPDCDP
                     RTVSPGGVKPVQGSEGQLAAAVAEHAANLDVTGLVDRLVFIDDSASRDGDPAITFSTV
                     SPSLLANQIDKAAVPMEFWAGWFDAGTADGALSRFMTYKTPMRLIIGPWNHGATQTVD
                     PYARSATTGVQPTVAEQYEQIFAFLKPYMIEGQRQPLAPEIRYYTLGAGTWRTTRQWP
                     PAGVVQRALYAADHGALVPAPPSGEAGGDTYRVDFSTSSGPANRWTTQLGGPVDYGDR
                     ASSAASRLNYTSPPLARPLEITGQPVVELWLTSSTPDGALHVYLEDVAPDGRVSYLTE
                     GEFRLIHRKPSPTPEPFRQFGPSHSFRREDAEPMPADRAQLVAFNMMPISVVIAQGHR
                     VRLSIAGADAAAFARIPATGPAPVYDVRRGGDAASRLLLPTAGPIILTATPPSSASVT
                     P"
     gene            complement(4299..5099)
                     /locus_tag="PLO65_01230"
     CDS             complement(4299..5099)
                     /locus_tag="PLO65_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD26903.1"
                     /translation="MTARSGGRQRPTDEPADSTVLVDIAEGVATVSLNRPARLNAINA
                     QLVSDLHQAMRDVQERSDVSAILLGGEGRAFCAGDDLTDIKSEFADAGRLAAMVDQLQ
                     DISRLIVLGPKPVVAAVHGWAVGGGLEWALNCDVTLFAETARGFFPEVRLGLAATGGV
                     TCLLPALVGPVRARELLYFGREFTAADAVGWGMISATAPDDQLMRVARSRAEQFRDLP
                     TPAVRELKAALGAPQRAAFEQALDTERDMLLRLAAIVVEGERWPEIKP"
     gene            complement(5096..6511)
                     /locus_tag="PLO65_01235"
     CDS             complement(5096..6511)
                     /locus_tag="PLO65_01235"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD26904.1"
                     /translation="MLDHVRTFATLVAAVMAFASCATAEPSSAPVGARLEAAAQAAFD
                     REGTAGLAVGVYCGDDVLLERGWGVADAGAAARVTPQTVFHVGSITKPFTGLAINQLI
                     AAGVLSPGDTVGRLLAGYVGPARDVTVAQLLTHTAGVPEYVTPEVVVDFSPYTRERAL
                     GLFKDRPLVFAPGSRWMYSNSDTYLLGLIIEAVSGLSYPDYVQQRIAAPFGLRHTWFA
                     GRTPATEPVAAGYFRTPNGPVVAPEYDPLLPFSAGSLLSTIGDLHRFVRAAYGPPGVA
                     KVAPGVRQALLAPVRLPDGEAVFYRLGSVAVLDMAGHTKVGHAGLTAGFTSYFAYYPD
                     DDLTIVVLSNATGVAPHPAHLEAELARIALGLPAPVIADRPTGAQAARDFAGDYAMVP
                     YSFFGTDVFGLSAREDGLYFQFGPADPKAPAIRLLRQDGDAFVSAADSEHVFRFRRDA
                     SGQVTGFDLSFYGMPFRGQRQ"
     gene            complement(6526..8673)
                     /locus_tag="PLO65_01240"
     CDS             complement(6526..8673)
                     /locus_tag="PLO65_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD26905.1"
                     /translation="MAEVDTIVVTAQKREQSLLDVPISVTAMTGEKLEIMGADQFADY
                     AASVPGLGFSSVGTFGNRGSRQINLRGISSNVGSATVGFYINETPLRFVDPRVVDLER
                     IEVLRGPQGTLYGAGAMGGAIRVITAKPSLTDFSGKAAGTLSYTENGESNYSLNGMVN
                     VPLVEGRAALRVTAFYDAEGGYIDNEPPEAMPLPATQVSTSAAQLVGRAQNYNDETIS
                     GYRVALTLEPNDSLTITPSVYHQRHEGDAWSAYIAEVGDLKADFLEMSPHAEDFTLYD
                     LTLNYDFGGAVLTSSTSFTDFSLLTTEPFERVLTAFFGAPYVAPYRSYIDQEVFVQEV
                     RLASSGDGPLTWLVGAFYQDDDQTTRQRFVAPDINDDLFGGFPVVSNGELFVFDAERK
                     ETQKAIFGEATYALSPKVDVTLGLRWFDIESTRVGVADGIFNGGQTLTNVTSNEDGWT
                     PKAEISFHPADDTLIYASVSKGFRPGFGFVVPPSPLCDADLASLGISSVSGQVESDSL
                     WSYETGGKTRVMDGRLTLAGSVYYNRWTNLQQSVELPTCGFVFSANAGAAESRGFELE
                     ATFRPIDALVFDLAVGYVNAELTEAAPGVAGAEGDKLLFVPDWNVALGVTYRHPVGET
                     DMTAFYHADYQYTGDMNFTFAPLPADLHSRDGYGIAGARVGLETPGWTAAAFVENLFD
                     ERPTVGNLRFATSGLLINTRRPRTVGISLSKSF"
     gene            8923..10473
                     /locus_tag="PLO65_01245"
     CDS             8923..10473
                     /locus_tag="PLO65_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD26906.1"
                     /translation="MDQPLTLPGLLRSAAARWGDREAIACELGDRVISFGELDAWSDR
                     LARVLVDRGVRTGEVVAVMLPNRIEFPVLWMAILKAGATMTPINPAYRGQDRNHLIVH
                     SGAVGFIGEDQAVASVEADRDQLEGLAWVATPEALSAAPAGDLPLPVAHPHATANIQY
                     TSGTTGMPKGCVLSHAYWIRFGEAFLHHGPRLTETDRILTAQAFSYADPQWNVVATLL
                     SGAALTILEKFSPSRFWAQVCAARTTFFYCLGAMPTLMLKTPPSTFDRAHQVRLVICS
                     AIPPDRHPDLEQRFGTPWLEAFGTTETGADLIVDMTEHAALVGSGSLGRPVRGKEMRV
                     LDIDGLPLPPGEVGELAIRGVGMMERYHADPEATARAFHGGSYHTGDLVRQDAEGRFT
                     YVGRTKDMIRRGGENVAAAEIEAVLQSHPDVLLAACVPARDEIYGEEIKAFLVLRRPT
                     ADGEGGPTVIRDIHDHVAARLARFKIPRYWEIVDSLPMTPSERVIKPTLVASETSPPR
                     GIDLGGRR"
     gene            10563..11243
                     /locus_tag="PLO65_01250"
     CDS             10563..11243
                     /locus_tag="PLO65_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD26907.1"
                     /translation="MTGETRLSAPETSYRKIIADRAEVMKLDKSQRTAMRLRAATADL
                     LETCRYNDLRVTDIAQKAQVAHGTFYTYYKQRSDIIFAIIQEYFEVLGETMRPTVGAD
                     EYSRIRSATGIFVGYYTSNLGLMRCLRALSEQDGQFAALKQQIDSSWFERNIAHLSRT
                     TLGASMERDDIRLMVYALGAMVEEFLYIRYQNPDPNVVRLTETEDDVAEKLARIWYRA
                     LYLRDPSD"
     gene            complement(11224..12270)
                     /locus_tag="PLO65_01255"
     CDS             complement(11224..12270)
                     /locus_tag="PLO65_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRD26908.1"
                     /translation="MSRAPAAPPLLSVGFLLLPQFTLTPFAGMVDVLRLAADVGDRSR
                     QIHCRWEVLSAGQAPIPASCGFEIRPTARLNTTDHFDYIVVVGGLLFDEPRLAPEEAA
                     FLRRRAAEGVRLIGVCTGSFEIIDLGLMRDRPCCVSWFHVADLLDRYPDARPVSDRLY
                     LDDGDRITCAGGAGAAQLCADLVARHCGAQQASKALRILMVDRLELGHGPQPPAGPSS
                     PSLSDRIRRALLVMEETIAAPLPIESIARRLGVSARQLERDFHDEFGYGPYAANLGLR
                     LDHARVLLSAGSAPITAVALACGFADASHMARRFRERFAATPTAYRRHVQERMRQARA
                     PAGQPPSAVSPTGP"
     gene            12515..12655
                     /locus_tag="PLO65_01260"
                     /pseudo
     CDS             12515..12655
                     /locus_tag="PLO65_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     assembly_gap    12656..12834
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12835..13677
                     /locus_tag="PLO65_01265"
     CDS             <12835..13677
                     /locus_tag="PLO65_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD26909.1"
                     /translation="NRVNGVDNEWLTPGQAKAFCPPLNIAAGARYPVLGAALQRRGGT
                     ARHDAVAWGYARAASALGVDIVQNCAVTGIRRGPDGAVQGVETTRGFIAASKIGVSAA
                     GATSTVMQMAGIRMPLESYPLQALVSEPVKPVMPCVVMSNTVHAYISQSDKGELVIGS
                     GTDQYVSYSQRGGLRAVTHAVEAVCELFPTFSRLRMMRSWAGTVDVTPDRSPIIGRTP
                     VPGLFVNAGWGTGGFKATPGSGHVFAWTIARDAPHPIAAPFSLDRFRTGRLIDEAAAA
                     AVAH"
     gene            13687..13986
                     /locus_tag="PLO65_01270"
     CDS             13687..13986
                     /locus_tag="PLO65_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRD26910.1"
                     /translation="MLLIDCPHCGPRAEPEFQYAGEAHIARPGDPASLSDEAWATHLY
                     YRSNTRGLHAERWRHTHGCGRFFNAVRDTVTDRFACTYPAGTTRPDRDGARGEPL"
     gene            13983..>14533
                     /locus_tag="PLO65_01275"
     CDS             13983..>14533
                     /locus_tag="PLO65_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD26911.1"
                     /translation="MTGAFRVEGCPAGDDRRVVRFSFNGRPLTGLAGDTLASALLANG
                     VHLMGRSFKYHRPRGVLTAGSAEPNALVTIDRGPGRVTPNVRATVQELHDGLRATSQN
                     HFPSLRFDLGAAADILSPLIPSGFYYKTFMWPRGAWRSLYEPAIRRMAGLGVAPSATD
                     PDRYAQRHAHCDVLVIGGGPAGLA"
BASE COUNT         2366 a         4957 c         4959 g         2072 t
ORIGIN      
        1 ccaaccgctc gccgcccccg ccggcggcgc gtccgccgcg gccgcctagc cccacagggc
       61 gggggtgaac aggtgtgacc gcccgtgctc gaaggtcatg ccgtgatcgc ggtcagcgac
      121 gtagtgggcc ggcccgtcga ggtcgcggta ggcgcacagg gacccgatga cataggtggg
      181 cgccatcgac agcgacgtgc ccatcatgtt gccgaccatg agctccagcc cgcggtcctg
      241 gcccagctcg gccagcttga gcgcttccgt cagccctccg gtcttgtcga gcttgatgtt
      301 gatcacgtcg tagaagggcg ccacgcgatc gaggtccgcg caggtgtgaa gggactcgtc
      361 gacgcagagc ttgatcggcg cctggaagcc cgccagcgct tcgtcctggc cgcgcgggac
      421 cggctgctcg atcatctcca ccccgagatc ggccaggccc ggcgcgacct cgcgcaacag
      481 atcgccggtc cagccttcgt tggcgtcgat caccagggtc gcccgggggg cgacggcgcg
      541 gatggcgttc atccgctcca tgatgcgatc ggcgttgagc ttgattttca gcgccgggaa
      601 ttcactcagc cgctcggcct ccccggccat cacggccggc tcgtccagac cgatggtcat
      661 cagggcgtcg aggaaaaccg gcgccggcag atcgagcaac gaccagatcc cgccggttcg
      721 cttggcctcg atgtcccaca gcgcgcagtc gatcgcgttg cgggccggcc cgggcggcag
      781 gagcgagcca agagcctcgc ggccgacgcc ttcctcgatc gcctggcgaa cgcgctcgat
      841 cgccgccagt tcgccggccg cggtctggtt gtagacgaag aagggcgatc cctccccgcg
      901 gccgatgacg ccgtcgtctt cgagcgtcac gaccaccaga tcggtcctgg tgatctcgcc
      961 cgaggcgatg cggaagcact tggtgagctc ccaggtctcg atccgggcgt ccatacgaag
     1021 agtcatgact ggtgttcccg caattgggct gcgtcccgaa ccgtcgggcc ggcctctagg
     1081 tgtggtcgaa gcccagttcg cgggccagtt cggcctccag cgagatggcc tgtcgtctga
     1141 gccagatccg gcgcagggcg tagtagaccg cgccgatggc gatgaaggcg gccccggccg
     1201 ccgtcgcacc cggactgccg gatgcggcga tgacgatgaa ggcgatcgac aggacgatca
     1261 ggccggtcga gaagaatacc agcaccggtc gcggaatccg gaaggcgctc gccaggtagc
     1321 gctcgcgatc acgccatggc agcacgagcg cggcgagacc gactccgacc tgaagcagca
     1381 tcaggccgac gacgacgaag gtggcgtagt cgacaatgcg cgcgccgaag gccagggcca
     1441 ccagggacag cacggtgatc gccaggatcg cggcgaccgg ctcgccggtt ctccggctcg
     1501 cgccggccag caggcgcggc agcgcccggc tccgggcgag ggcgagcaga tcccgcgaat
     1561 agcccatcag catgacgttg atcgaactgg ccgtcgccgc cacggccgag aaggcgatca
     1621 cctgcacgac cccgttcggc aggaccctgg cggcggccga gctcaccggc gcctcgacgc
     1681 gcgccagatc ggtccaggga agctgggcga cgaccgccag ggccaccagc gcgtagacca
     1741 tcgccacgat gacaaagctg atcgccaggg cgagcgggat attgcgcgcc ggccggcgga
     1801 cctcgccgct gagttcgatg atcaccatga agccggcgaa ggaaaaatag gccggaatgg
     1861 cgcctgaaat gaccggtccc aggccgttgg gcaggaacgg cgacaggggc cgcagctcgc
     1921 cccccgtcag ggccgcggca gcaaacagca ggagcgcgac gagaaagaag gccaccatca
     1981 gggtctgggc ggcgatcgtg tcgcgcaccc cgatcagatt cagcccgccg agaccgacga
     2041 tcagggccgt cgccaggacc agccggtcga cacccgggac ccacagccgc acatagtcgg
     2101 cgaatccgaa gcccagcagc gcgacggcga ccacagccgc gccgatcatg ccccagacgc
     2161 acaggaaggc gaacgccggc gacaccagcc gtccaatcgc cacgaagctc gcgcccgaga
     2221 ccgggaacac cgagccgagc tgggccgcga cgacacagga gaacaccgcc agcacggccg
     2281 cgccggcgta ggagagcacc acgccggggc cggtcgtggc ggcgatctca cccggcagga
     2341 tgtagatcga ggcgccgaca acgaatccga cgatggtgac caccgcgccg aacgcgccga
     2401 ttcccttgcc cgacgacgcc ccgctcatgg cgtcacgctg gcggacgagg gaggcgtcgc
     2461 cgtcaggatg atcggtccgg ccgtcggaag cagcagtcgc gaggccgcgt cgccgccgcg
     2521 ccggacgtca tagaccgggg ccggacccgt ggccgggata cgggcgaagg ccgccgcgtc
     2581 ggcgccggcg atcgacaggc ggacccggtg accctgcgcg atgaccaccg agatcggcat
     2641 catgttgaag gcgaccagct gggcgcggtc cgccggcatc ggctcggcgt cctcgcgcct
     2701 gaagctgtgc gacggtccga actggcgaaa cggctccggc gtcggcgacg gcttgcggtg
     2761 aatcagcctg aactcgcctt cggtcaggta gctgacacgg ccgtcgggag ccacgtcctc
     2821 gagatagacg tgcagggcgc cgtcgggggt cgaggaggtc aaccatagct cgacgaccgg
     2881 ctggccggtg atctccagcg gcctggccag gggcggcgag gtgtagttga gccgggaggc
     2941 ggccgacgaa gcgcggtcgc cgtagtcgac cggcccgccc agctgggtgg tccagcgatt
     3001 ggccgggccc gaggaggtgg agaagtcgac ccggtacgta tccccgcccg cttcgccgga
     3061 aggcggagcc gggacgagcg cgccatgatc ggccgcatag agggcgcgct gcaccacccc
     3121 ggccggcggc cactggcggg tcgtccgcca ggttccggcg ccaagcgtgt agtagcggat
     3181 ctcgggcgcc aggggctggc gctgaccctc gatcatgtag ggtttgagaa aggcgaagat
     3241 ctgttcgtac tgctcggcca ccgtcggctg gacgcccgtg gtcgccgacc tcgcgtaggg
     3301 atcgacggtc tgggtcgcgc catggttcca cggcccgatg atcagccgca tgggggtctt
     3361 gtaggtcatg aaccggctca gggccccgtc cgccgtgccc gcgtcgaacc agccggccca
     3421 gaactccatc ggcacggcgg ccttgtcgat ctggttggcc agcagggagg ggctgacggt
     3481 cgagaaggtg atggccgggt cgccatcgcg actggcgctg tcgtcgatga acacgaggcg
     3541 gtcgacgagg cccgtgacgt cgagattggc ggcatgttcg gccacggcgg ccgcaagctg
     3601 cccctcactg ccctgcacag gcttgacgcc acccggcgac accgtgcgtg gatcacagtc
     3661 cgggcccgcc gccacgcacg gatcgtcgcg gtccagcgcc ccgacgatct caccccaggc
     3721 cttgagcagg accgtgttcg gcacgccgcc gggaaagacg gcgtaccggt aggtgtcgaa
     3781 atcgctgaac cggggaaccg ccgccctgag cgcgtcgccg ccatgaatgg cggccagctc
     3841 ggcggtgttt cccagatagg aagaccccgt cgtcgccact cgcccgttcg accagggctg
     3901 ggcggcgatc caggcgatga tctcaccgta gtcccggatt tcatccggcg agaattccgt
     3961 cacgcgggag ccgaacgagg cgcccgaccc acggacgtcg acgatggcca acgcgtaccc
     4021 ggccttggcg aacagccgcg cgcgctcgaa attgacgtcg gcctcgggcg cgggggaggc
     4081 cacgacccgc gagcgccagt agcgcgtcag gagcaggacg gtcggcgcgc cctcggctcc
     4141 gacatccggc ggcgtccaga catcgacggc gatgcgcacg ccatcgcgca tctcgatata
     4201 ctgagaggtc agggctggcg tctgcgacca ggccgcctgg gggacgaacg ccaggacgac
     4261 caccagggcg agcatgcgcg ccagggccct cgcccgtgtc atggcttgat ctccggccac
     4321 cgctcgccct cgacgacgat cgccgccagt ctgagcagca tgtcgcgttc ggtgtcgagc
     4381 gcctgctcga aggcagctct ctggggcgct cccagggcgg ccttcagctc gcgcaccgcc
     4441 ggcgtcggca gatcgcgaaa ctgttcggcc cgcgaccgcg ccactcgcat cagctggtcg
     4501 tcgggcgcgg tggccgagat catcccccat ccgaccgcgt cggcggccgt gaactcgcgg
     4561 ccgaagtaca gcagctcccg cgcccgcaca gggcctacga gagccggcaa caaacaggtg
     4621 acgcctccgg tagccgcaag gccgagccgg acttcgggaa agaagccccg ggcggtctcc
     4681 gcgaacagcg tgacatcgca gttcagcgcc cactccagcc cgcccccgac cgcccagcca
     4741 tgaaccgccg cgacgaccgg cttggggccc aggacgatga gccgggagat gtcctgcagc
     4801 tggtcgacca tcgccgcgag ccgcccggcg tcggcgaact cgctcttgat gtcggtcagg
     4861 tcgtcgccgg cgcagaaggc gcgcccctcc cctcccagca ggatcgcgct gacgtcggac
     4921 cgctcctgaa catcccgcat cgcctgatgc aggtcgctga cgagctgggc gttgatggcg
     4981 ttgaggcggg ccggcctgtt cagcgagacc gtcgcgactc cctcggcgat atcgaccagc
     5041 accgtcgagt cggcgggctc gtcggtcggt ctctgcctgc ccccggacct ggccgtcatt
     5101 ggcgctgccc gcggaacggc atgccgtaga agctcaggtc gaaaccggtg acctgtcctg
     5161 aggcgtcgcg ccggaagcgg aacacatgtt cgctgtccgc cgcgctgacg aacgcgtcac
     5221 catcctggcg cagcagccgg atggccggcg ccttcggatc ggccgggccg aactggaagt
     5281 agaggccgtc ctcccgggcg gaaaggccga acacgtccgt gccgaagaag ctgtagggga
     5341 ccatggcgta gtcgccggcg aagtcccggg ccgcctgggc cccggtcggc cggtcggcga
     5401 tgaccggcgc cggcagacca agggcgatgc gcgcgagttc ggcctcgaga tgggccggat
     5461 ggggggccac ccccgtggcg ttggacagca ccacgatcgt caggtcgtcg tcgggatagt
     5521 aggcgaagta gctggtgaac cccgccgtca ggccggcgtg accgaccttg gtgtggccgg
     5581 ccatgtccag caccgcgact gatccgaggc ggtagaagac cgcctcgccg tcgggcaggc
     5641 gcaccggggc gagcagcgcc tgacggacgc cgggcgcgac cttcgccact ccgggaggac
     5701 cgtaggcggc gcgaacaaat ctgtgcagat cgccgatcgt cgacagcaac gagcccgccg
     5761 agaaaggcag taggggatcg tactccggcg caaccacagg gccgttcggc gtgcggaaat
     5821 acccggcggc gaccggctcg gtcgccggcg tccgtcccgc gaaccaggtg tgccgcaggc
     5881 cgaatggcgc ggcgatccgt tgctggacat agtcagggta gctgagcccg ctgaccgcct
     5941 cgatgatcag gcccagcagg taggtgtcgg agttcgagta catccagcgg gagcccggcg
     6001 cgaagaccag cggccggtcc ttgaacaggc ccagggcccg ttctcgcgtg taggggctga
     6061 aatcgacgac cacctcgggc gtcacatatt cgggcacgcc ggcggtgtgg gtcagcagtt
     6121 gcgcgaccgt gacgtcgcgg gccggcccga catagccggc gagcagcctg cccaccgtat
     6181 cgccgggtga aaggacgccc gcggcgatca gctgattgat cgccaggccc gtgaagggct
     6241 tggtgatcga tccgacatga aagacggtct ggggcgtgac ccgcgcagcg gcgccggcgt
     6301 cggccacgcc ccatccccgt tcgagcagaa catcgtcccc gcagtagaca ccaaccgcca
     6361 gacccgcagt tccctcgcgg tcgaaggcgg cttgcgcagc cgcctccagc cgcgccccca
     6421 ccggagccga ggagggttcg gccgtcgcgc aggaagcaaa ggccatgact gccgccacca
     6481 gcgtggcgaa ggtcctgacg tggtccaaca tcggggtctg ctcgcctaga acgacttgct
     6541 gaggctgatg ccgaccgtac gcggccggcg ggtgttgatc agcaggcccg aggtggcgaa
     6601 ccgaaggttg cccaccgtcg gtcgctcgtc gaacaggttc tcgacgaagg cggcggctgt
     6661 ccagcccggg gtctcgagcc ccacccgggc gccggcgatg ccgtagccgt cccgcgaatg
     6721 caggtccgcc ggcagcggcg cgaaggtgaa gttcatgtcg cccgtgtact ggtagtcggc
     6781 gtggtagaag gccgtcatgt cggtctcgcc gaccggatgc ctgtaggtga cgccgagcgc
     6841 gacattccag tcgggcacga acagcagctt gtcgccctct gccccggcca ccccgggggc
     6901 cgcctcggtc agctcggcgt tgacgtagcc caccgccaga tcgaacacga gcgcatcgat
     6961 tgggcggaag gtcgcctcga gttcgaagcc gcggctctcg gccgcgccgg cgttggcgga
     7021 gaaaacgaag ccgcaggtgg ggagctcgac gctctgttga aggttggtcc agcggttgta
     7081 gtagaccgag cccgcgagcg tcaggcggcc gtccatgacg cgggtcttgc ctcccgtctc
     7141 gtatgaccac agggagtcgg actcgacctg gccgctgacg ctggaaatgc ccagggaggc
     7201 cagatccgcg tcgcacagcg gacttggcgg aacgacgaag ccgaagccgg ggcggaagcc
     7261 cttggacacg ctggcgtaga tcagcgtgtc gtcggccggg tggaagctga tctcggcctt
     7321 cggggtccag ccgtcctcgt tggaggtgac gttcgtcagg gtctggccgc cgttgaagat
     7381 gccgtcggcg acgccgacgc gggtcgattc gatgtcgaac cagcgcagtc cgagcgtgac
     7441 gtccaccttc ggcgacagag cgtaggtcgc ctcgccgaag atcgccttct gggtctcctt
     7501 gcgttccgcg tcgaaaacga agagctctcc gttcgagacg accgggaacc cgccgaacag
     7561 gtcgtcattg atgtcgggcg cgacgaaccg ctgccgggtc gtctggtcgt catcctgata
     7621 gaaggcgccc accagccagg tcaggggacc gtcgccgctc gacgcgagcc ggacttcctg
     7681 aacgaatact tcctgatcga tgtacgaccg gtagggcgcg acgtagggcg cgccgaagaa
     7741 ggcggtcagc acccgttcga acggttccgt cgtcagcagg ctgaagtcgg tgaaggaggt
     7801 cgacgaggtc agcaccgccc cgccgaagtc gtagttcagc gtcaggtcat agagggtgaa
     7861 gtcctccgcg tgcggcgaca tctcgaggaa gtcggccttc aggtcgccga cctcggcgat
     7921 gtaggccgac caggcgtcac cctcatggcg ctgatgatag acgctgggcg tgatcgtcag
     7981 gctgtcgttg ggctcgagcg tcagggcgac acgatagccg gagattgttt cgtcgttgta
     8041 gttctgcgcc ctgccgacca gctgggcggc cgacgtcgac acctgggtcg ccggcaaggg
     8101 catggcctcg ggcggctcgt tgtcgatata gccgccctcg gcgtcataga atgcggtcac
     8161 gcgaagcgcc gcccgaccct cgaccagcgg cacattgacc atgccgttga gcgagtagtt
     8221 ggactcgccg ttttccgtgt aggacagcgt gcccgccgcc tttcccgaga aatccgtcag
     8281 gctcggcttg gcggtgatca cccggatggc gccgcccatg gcgccggcgc catagagggt
     8341 gccctgcggc ccgcgcagca cctcgatccg ctccagatcg acgacccggg gatcgacgaa
     8401 ccgcagcggc gtttcgttga tgtagaagcc gaccgtcgcc gacccgacgt tcgaggagat
     8461 gccgcgcagg ttgatctggc gcgagccgcg attgccgaac gtgcccacgg acgagaaccc
     8521 gagacccggc accgacgcgg cgtagtcggc gaactggtcg gcccccatga tctccagctt
     8581 ctcgccggtc atcgccgtaa ccgaaatcgg cacatccagc agcgactgct cgcgcttctg
     8641 ggccgtcacg acgatggtgt cgacctcggc cacctgtgcg tgcgcaacgg tcgcgcaagt
     8701 catggccaac gcaatagccg cggcgctgtt gaggcacgcc ctgcgaaccc tactcgacat
     8761 gagacccctc cttcatggac gtcgacgcac ccccccgggc acggcgtttc acgtcctggc
     8821 gacggcgaac gtctcccgac ttgacgttga tgtcaattcc aattttgctg tcccgcgtcg
     8881 ccggctctat aggctggccc gcgctttcca gaggacgaca ccgtggacca gccccttacc
     8941 ctgccaggcc tgttgcgatc ggccgccgcg cgctggggcg accgtgaggc catcgcctgc
     9001 gagctgggcg atcgcgtgat ttcgttcggc gaactcgacg cctggagcga tcggctggcc
     9061 cgggtcctgg tcgaccgggg cgtccggacc ggcgaagtcg tggccgtgat gcttcccaac
     9121 cggatcgagt ttccggtgct gtggatggcc atactgaagg ccggggcgac gatgacgccg
     9181 atcaacccgg cctaccgggg gcaggaccgg aaccacctca tcgtccattc cggcgcggtc
     9241 ggtttcatcg gcgaagacca ggccgtggcg tcggtcgagg ctgaccggga ccagctggaa
     9301 gggctggcgt gggtcgccac gcccgaggcg ctctccgcgg cgccggcggg cgacctgccg
     9361 cttccggtgg cgcatcccca cgccacggcc aacatccagt acacctcggg caccaccggc
     9421 atgcccaagg gttgcgtcct ctcgcacgcc tactggatcc gcttcggcga ggcctttctg
     9481 catcacggcc cgcgcctgac cgagacggac cgcatcctaa cggcccaggc cttcagctac
     9541 gccgacccgc agtggaacgt cgtcgcgacg ctactgtccg gcgccgcgct gaccatcctt
     9601 gagaaattca gcccaagccg cttctgggca caggtctgcg cggccaggac gaccttcttc
     9661 tactgcctgg gcgccatgcc cacgctgatg ctgaagacgc cgccatcgac gttcgatcgc
     9721 gcccaccagg tgcggctggt gatctgctcg gccattcccc cggaccggca tcccgacctg
     9781 gagcaacggt ttggaacgcc ctggctggaa gccttcggca cgaccgagac cggggccgat
     9841 ctcatcgtcg acatgaccga gcatgcggcc ctggtggggt ccggttcact ggggaggccg
     9901 gtgaggggca aggaaatgcg agtcctcgac atcgacggcc ttcccctccc ccccggggaa
     9961 gtgggcgaac tggccatcag gggcgtgggc atgatggaga ggtatcatgc cgatcccgaa
    10021 gcgaccgccc gggcgttcca tgggggatcc taccacaccg gcgaccttgt ccggcaggac
    10081 gccgaggggc gcttcaccta tgtcggccgg accaaggaca tgatccggcg tggcggggag
    10141 aatgtcgccg cggcggagat cgaggccgtc ctgcagtccc accccgatgt tctgctcgcc
    10201 gcctgcgttc ccgcgcggga cgaaatctac ggcgaggaga tcaaggcctt cctggtcttg
    10261 cgccggccga ccgcggacgg cgaaggcggg ccgacggtga tcagggatat ccacgaccat
    10321 gtcgcggccc ggctcgcgcg gttcaagatc ccccgatact gggagatcgt cgactccctg
    10381 cccatgacgc cctcggagcg ggtgataaag ccgacgctgg tcgcctcgga aaccagccct
    10441 ccccgaggaa ttgatttggg cgggcgccgt tgatttcccg gcgattctga gcacccttgc
    10501 cggatgaagc tacgccagac gcaaggacag gggcggcccg tcgcgatcgg ccccggacag
    10561 ccatgacagg cgaaacccgg ctgtccgccc cggaaaccag ctaccggaaa atcatcgccg
    10621 accgcgcgga ggtgatgaag ctcgacaagt cgcagcgcac ggccatgcgg ctgcgcgcgg
    10681 cgaccgccga cctgctggag acctgtcgct acaatgacct gcgggtgacc gatatcgccc
    10741 agaaggcgca ggtcgcccac ggcaccttct acacctacta caagcagcgc tcggacatca
    10801 tcttcgccat catccaggag tatttcgagg tcctgggcga gaccatgcgg ccgacggtgg
    10861 gagccgacga gtactccaga atccgttcgg ccacgggcat cttcgtcggc tactacacct
    10921 ccaacctcgg cctgatgcgc tgccttcggg cgctgagcga gcaggacggc cagttcgccg
    10981 ccctgaaaca gcagatcgac tcaagctggt tcgaaagaaa tatcgcccac ctgtcccgca
    11041 ccacgctggg cgcctcgatg gagcgggatg acatccggct gatggtctat gccctcggcg
    11101 cgatggtcga ggagtttctc tacatccgct accagaatcc ggaccccaac gtcgtccgac
    11161 tgaccgagac ggaagacgac gtcgccgaga agctcgcgag aatctggtac cgcgcgctct
    11221 atctcaggga cccgtcggac tgaccgccga cggtggctga cccgccgggg cccgcgcctg
    11281 ccgcatccgc tcctggacgt gccggcgata ggctgtcggc gtggcggcga accgctcccg
    11341 gaaccgcctg gccatgtgcg aggcgtcagc aaatccgcag gccagggcga ccgcggtgat
    11401 cggcgccgag cccgccgaca gcagaaccct ggcgtggtcc agacggagcc ccagattggc
    11461 ggcgtagggg ccgtaaccga actcgtcgtg gaaatcccgc tccagctgcc gcgccgagac
    11521 gcccagcctg cgggcgatcg attcgatcgg cagcggcgcg gcgatcgtct cctccatcac
    11581 caggagcgcg cggcggatgc gatcggagag agacggcgac gacggccccg ccggcggttg
    11641 tggcccatgg ccaagctcca accggtcgac catcaggatc cgcagcgcct tgctggcctg
    11701 ttgcgcgccg cagtgccgcg ccaccaggtc ggcgcacagt tgggccgcgc cggcgccgcc
    11761 cgcgcaggtg atgcgatctc cgtcgtcgag atacagccgg tcggacaccg gtcgcgcatc
    11821 cggatagcgg tcgagcaggt ctgcgacatg gaaccagctg acgcagcagg gccggtcgcg
    11881 catcagaccg aggtcgatga tctcgaagct gccggtgcag accccgatca ggcgcacgcc
    11941 ttcggccgcc cggcggcgca ggaacgcggc ctcctcgggc gccaggcgcg gctcgtcgaa
    12001 cagcagtccg ccgaccacca cgatgtagtc gaaatggtcc gtcgtgttca gccgggcggt
    12061 cgggcggatc tcgaacccgc agctggccgg gatcggcgct tgcccggccg agagcacctc
    12121 ccaccggcaa tggatctgcc ggctgcggtc gccgacatcc gccgccagcc gcaggacatc
    12181 caccatgccg gcgaaggggg tcagggtgaa ctgcggcagc agcaggaagc cgaccgacag
    12241 caggggcgga gccgcagggg ctctggacat gggagaggtc tttcggcgcg gaagcacggc
    12301 ggcggcgcgg tgatgtcgga aatggacgga gaactgtcgc ttctgtcaat gcggaaaccc
    12361 gacgacctgt ttaccggtga cacaggccca atggcgggcg tcatgcgggg acgacatggc
    12421 cggacgtttc tcagccctgt ccctgctgcg ccaggcggcc ggcggcggcc agcgtctggc
    12481 gccgcaatgg cgcgacgccg cgcccgcggc ggcctatgac gtggtcattg tcggcggcgg
    12541 cgggcacggc ctggccgccg cctattacct ggcgaaggag catggcgtcc gccgggtcgc
    12601 ggtgctcgaa aggggctgga tcggcggcgg caacaccggt cgcaacacca ccatcnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatcgc
    12841 gtgaacggcg tcgacaacga atggctgacg cccggccagg ccaaggcctt ctgcccgccc
    12901 ctgaacatcg ccgccggcgc ccgctacccg gtcctcggcg ccgccctgca gcggcggggt
    12961 ggaaccgccc ggcatgacgc tgtcgcctgg ggctatgcgc gcgcggccag cgcgctcggc
    13021 gtcgacattg tccagaactg cgccgtgacc ggcatccgcc gcggccccga cggcgcggtg
    13081 cagggtgtcg agacgacccg cggcttcatc gcggccagca agatcggcgt gtcggccgcc
    13141 ggggcgacca gcacggtaat gcagatggcc ggcatccgca tgccgctcga gagctatccc
    13201 ctccaggccc tggtttccga accggtgaag ccggtcatgc cctgcgtggt catgtccaat
    13261 accgttcacg cctacatcag ccagtccgac aagggcgagc tggtgatcgg ctcgggaacc
    13321 gaccagtacg tctcctattc gcagcgcggc ggcctgcggg ccgtgaccca tgccgtggaa
    13381 gccgtgtgcg aactgttccc gaccttctcg cggctgcgga tgatgcgcag ctgggccggc
    13441 actgtcgatg tcacgcccga ccgctcgccg atcatcggca ggacgcccgt gccggggttg
    13501 ttcgtcaacg ccggctgggg caccggcggc ttcaaggcca caccgggctc gggccatgtc
    13561 ttcgcctgga ccatcgcccg ggacgcgccc catccgatcg cggcgccatt ctcactcgat
    13621 cgcttccgca cgggtcgtct catcgacgag gcggcggcgg cggccgtcgc gcactaggac
    13681 cccgtcatgc tgctcatcga ctgtccgcat tgcggccccc gcgccgagcc ggaattccag
    13741 tatgccggcg aggcccatat cgcgcggccg ggcgacccgg ccagcctgtc cgacgaggcc
    13801 tgggcgaccc acctctacta tcgatccaac acccgcgggc tccacgccga gcgctggcgt
    13861 cacacccatg gctgcggccg gttcttcaac gcggtccggg acacagtcac cgaccggttc
    13921 gcctgcacct atccggcggg gacgacccgg ccggacaggg acggcgccag gggtgagccg
    13981 ctatgaccgg agcgttcagg gtcgaaggat gtccggcggg cgacgaccgc cgtgtggtcc
    14041 ggttctcgtt caacgggcga ccgctcacgg gcctggccgg cgacaccctg gcgtccgccc
    14101 tgctcgccaa cggcgtccat ctgatgggcc gatccttcaa gtaccaccgg ccgcgcggcg
    14161 tgttgacggc gggttcggcc gagcccaacg ccctggtcac gatcgatcgc ggccccggcc
    14221 gggtgacccc caacgtccgg gccaccgtgc aggagcttca tgacgggctg cgcgcgacga
    14281 gccagaacca cttcccctcg ctgcggttcg atctgggcgc cgcggcggat atcctgtcgc
    14341 cgctgattcc gtcgggcttc tactacaaga ccttcatgtg gccgcgcgga gcgtggcggt
    14401 cactgtatga gccggccatt cgcaggatgg ccgggctggg cgtcgccccg agcgcgactg
    14461 acccggaccg gtacgcccag cggcatgcgc actgcgacgt gctggtgatc ggcggcgggc
    14521 cggccgggct ggc
//
LOCUS       DAORNX010000015        14296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3210_length_14296_cov_8.674882, whole genome shotgun sequence.
ACCESSION   DAORNX010000015 DAORNX010000000
VERSION     DAORNX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_14296_cov_8.674882"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLO65_01280"
     CDS             complement(<1..688)
                     /locus_tag="PLO65_01280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD26912.1"
                     /translation="MKYRADVDGLRAVAVLAVVGFHAGAPWLTGGFAGVDVFFVISGF
                     LIGGVVAERQARGDFSLGWFWERRVRRIFPSLMVMLAATTAVAWWLLTPGDLLAYVRS
                     MAGALVSLSNVHFWRTSDYFGGEDLVRPLLHTWSLGVEEQFYLVFPVLMLLLARVAAG
                     RIRLVLAGLGALSLALAVWQTAAHPDAAFYLPLARAWELLAGVLLALWAPRVRPGVPQ
                     HALGLTGLALI"
     gene            complement(718..1509)
                     /locus_tag="PLO65_01285"
     CDS             complement(718..1509)
                     /locus_tag="PLO65_01285"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD26913.1"
                     /translation="MTGRVAGKVVLVTGGASGIGRGCAELLAREGAAVVITDVQDDMG
                     ADVARALGGKAVYLHHDVTEEDAWIGVVTEVGKRFGRLDVLVNNAGVGMGGPVTEFSL
                     ADWRKQQGVNVEGVFLGVKHSLPLMRQGGGGSIINMSSIAGLTGAANMSSYCATKGAV
                     RLFTKAVAMECATARDGVRVNSVHPGIIETPIWDTIGGTIGGEGGNAGPARAIDLDDM
                     TAVATPLGVKGFPADIAAGVLFLASDESRYMTGAELVIDGGMTAR"
     gene            complement(1680..2345)
                     /locus_tag="PLO65_01290"
     CDS             complement(1680..2345)
                     /locus_tag="PLO65_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD26914.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGLPYEIRHYQRDAKTMLAPPEL
                     KAIHPLGKSPVITDGPATLAETGAIVETIVEAYGGGRLKPPAGSEDRLRWLYWLHYAE
                     GSAMPPLLMKLVFTALPDRAPGLIRGIVRGISSQALKSFVDPQLKAHFDYWEAELGKA
                     GWFAGKDFSAADIMMSFPVEAAADRAGALVGRPRLTAFLEKIHARPAYRAALEKGGPY
                     AYA"
     gene            complement(2444..2803)
                     /locus_tag="PLO65_01295"
     CDS             complement(2444..2803)
                     /locus_tag="PLO65_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD26915.1"
                     /translation="MPTPARVAAFVAMVEDGRFVEAIETFYATGATAQENLGRTSVGR
                     AALVEKERAALTRNDIVARKGSVVLVDGDRVAINWVFEMTDDAGTTRVLDEIAWQRWD
                     GDRIVEERFYYDPGQLR"
     gene            complement(3045..3236)
                     /locus_tag="PLO65_01300"
                     /pseudo
     CDS             complement(3045..3236)
                     /locus_tag="PLO65_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
     gene            complement(3344..6631)
                     /gene="carB"
                     /locus_tag="PLO65_01305"
     CDS             complement(3344..6631)
                     /gene="carB"
                     /locus_tag="PLO65_01305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRD26916.1"
                     /translation="MPKRTDISSILIIGAGPIVIGQACEFDYSGVQACKALRAEGYRI
                     ILVNSNPATIMTDPDVADATYIEPITPEMVAKIIEKERPDALLPTMGGQTALNTALSL
                     ESMGVLAKFGVEMIGAKAEVIDKAEDRQKFRDAMDRLGLESPRSKAAHTLEEAREGLA
                     YVGLPAIIRPSFTLAGTGGGIAYNLEEFEEIVLRGLDLSPTTEVLIEESVLGWKEYEM
                     EVVRDRADNCIIVCSIENVDPMGVHTGDSITVAPALTLTDKEYQRMRKGSIDVLREIG
                     VETGGSNVQWAINPKDGRMVVIEMNPRVSRSSALASKATGFPIAKVAARLAVGYTLDE
                     LANDITGGAMPASFEPSIDYIVTKIPRFAFEKYPGSEPHLTTSMKSVGEVMAIGRTFA
                     ESLQKALRGLETGLSGLDEIDIPGTSDPDTAHAAVLRALAMPTPERLLVIAQAFRHGL
                     TVEEIHGACSYEPWFLRQIAEIVRQEGKVRASGLPAADGLRKLKAMGFSDARLAKLTS
                     LTELEVRARRHAMNIRPVFKRIDTCAGEFAATTPYMYSTYETGALGQAPDCEAEPSSR
                     KKAIILGGGPNRIGQGIEFDYCCCHAAFALDAIGIESIMVNCNPETVSTDYDTSDRLY
                     FEPLTAEDVLELIHVEQKAGTLAGVIVQFGGQTPLKLAHPLEQAGIPILGTSPDAIDL
                     AEDRERFQQLLHRLDISQPVNALARSAEECFTAAAEVGYPIVIRPSYVLGGRGMEIIR
                     DDEQLERYVRTAVEVSGDAPILIDQYLSRATEVDIDALCDGTDVFVAGVLEHIEEAGV
                     HSGDSACSMPPFSLRPDIVAELKRQTVEMALALNVRGLMNVQFAIEEPHSAAPRIFVL
                     EVNPRASRTVPFVAKTIGAPVAAIAARIMAGETLASFGLVDKPYDHIAVKEAVFPFAR
                     FAGVDTVLGPEMRSTGEVMGLDWKRDGEADMAPAFARAFAKSQLGGGVTLPTAGTAFV
                     SVKDSDKIWIIEPVRLLQAAGFRVLTTAGTGEWLRSQGVETEHVRKVLEGRPHIVDAM
                     KNGEVQLVFNTTEGKQSLADSFELRRTALMMKIPYYTTSAGALAAAQAITSAPLAALE
                     VRALQSF"
     gene            7021..7452
                     /locus_tag="PLO65_01310"
     CDS             7021..7452
                     /locus_tag="PLO65_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26917.1"
                     /translation="MPYREKSNLLALAAMLIAYVPYFVVAAMTPVQTVVTPDLLLLTL
                     FAAVSVARLLILGVGYLWFRVTTPDDVREPADERDRAITRRSTTVAYYVLMAGTLVAA
                     AVMPVWFAGWTIVNAALFAIVLAEVIRCLVAAVSYRRGWNG"
     gene            7445..7669
                     /locus_tag="PLO65_01315"
     CDS             7445..7669
                     /locus_tag="PLO65_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD26918.1"
                     /translation="MAEGAITNQIRTLRFLAGEMSQAELGQKIGVTRQTIAAIEAGKY
                     SPTLEAAFRIAEVFGKPLDEVFQWIPGGQR"
     gene            complement(7660..8850)
                     /locus_tag="PLO65_01320"
     CDS             complement(7660..8850)
                     /locus_tag="PLO65_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRD26919.1"
                     /translation="MTTTTMDGAMATPARPPRLTSVDMLRGLVIVIMALDHTRDYFHS
                     QAFLFDPTDAGATSLAVYLTRWVTHVCAPTFVLLAGMSAFLQGQAGKGRGALARFLVS
                     RGLWLIFLEMVLLNYGWNFTIYGFGLQVIWALGVGMIALAGLIWLPRGAVLAIGVAIC
                     AGHNLLDPVRPADLGPAAPLWVMLHEGGPTLTAFFIVYPVLPWIGVMALGYGLAPVLT
                     WTPRRRARALIALGVAMIAAFLVLRGVNAYGDPRPWKALVEPMRRGFSFFDVTKYPPS
                     LDYLLITLGSMLALLPAMERLKGPVARVLEVYGRAPLMFYIAHIYLLHLAMLLTGLAM
                     GLPAQTFVWIMADPSPLIRAHWGFPLPVVYGVWIVATASLYPLCRWWGDLKRRRRDWW
                     LSYL"
     gene            8997..9350
                     /locus_tag="PLO65_01325"
     CDS             8997..9350
                     /locus_tag="PLO65_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26920.1"
                     /translation="MRILLGAVAALALSATAALAGGDDIMATRFGNTTITTDASGQSS
                     RIYYEADHSFTGVQGDQQLAGTWEVKGSQVCLTFSSDAPPGYPNPICTAVSAHNVGDS
                     WTSGPFTVQLVAGHQ"
     gene            complement(9445..10614)
                     /gene="carA"
                     /locus_tag="PLO65_01330"
     CDS             complement(9445..10614)
                     /gene="carA"
                     /locus_tag="PLO65_01330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRD26921.1"
                     /translation="MPQTVLPGVTGVLALADGTILQGIGVGAAGDAVGEVCFNTAMTG
                     YQEILTDPSYMAQIVAFTFPHMGNVGTNVEDLEQISGGSETAARGAIFRDVPTDPANW
                     RADGDLPGWMRRRGVIGLAGVDTRALTRTIREKGMPHAVIAHSPDGVFDLDALVAKAR
                     AWSGLEGLDLAKDATTRQSFVWDEGLWDWPEGYARPDAPKYEVVVLDYGVKRNILRAL
                     SSIGARATVVPADTSAEAILARKPDGVLLSNGPGDPAATGEYAVPEIRKLVDSGTPVF
                     GICLGHQMLALALGARTVKMEQGHHGANHPVKDLTTGKVEIVSMNHGFTVDRDSLPAP
                     VTETHVSLFDGTNAGLALADKPVFSVQHHPEASPGPTDSLYLFERFAGLMDRAKA"
     gene            10696..10938
                     /locus_tag="PLO65_01335"
     CDS             10696..10938
                     /locus_tag="PLO65_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HRD26922.1"
                     /translation="MLQMRPNCECCDRDLAPNGGDARICSFECTFCADCALDRFDGRC
                     PNCGGDLVVRPPRPATLLAKYPASTERVVKDHGAAT"
     gene            11088..11786
                     /locus_tag="PLO65_01340"
     CDS             11088..11786
                     /locus_tag="PLO65_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD26923.1"
                     /translation="MSITTVGLDADDTLWHNETLFRLTHRRFTDLLAPHCDEATLEAR
                     LAETEKRNLRLYGYGVKGFTLSMIETAMELTDGAVETAVIREILAAGREMLTEPVEPL
                     PGVDAALAQLSERYRLVLITKGDLLHQEQKLAASGLGDLFAAVEIVSEKDADTYRRVF
                     ARHGTGPEQAVMAGNSMRSDILPALDAGCWGALIPYPLVWAHEAAEAPADHDRYVELA
                     SISDLPGWVDGVAG"
     gene            11902..13656
                     /locus_tag="PLO65_01345"
     CDS             11902..13656
                     /locus_tag="PLO65_01345"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRD26924.1"
                     /translation="MRLAPFTALLSAVLLLSWSSTSTAALPWARSKAVPFDIVIRNGT
                     VYDGSGGAPFVGDVAIRGDRIVAVGKKLRGPRLTEIDATGLAVSPGFINMLSWATESL
                     LADGRGLSDLKQGVTLEVMGEGSSMGPWNEKMRRQELARQGDIKFDISWTTLDGYLSH
                     LETLGIAQNVASFVGAETVRTNILGEEDVDPTPAELARMRALVVQAMEEGALGVGSSL
                     IYAPGSYADTDELVALSTEAGRCGGMYISHMRSEGNRLLEAIDELIDISRRSGAPAEI
                     YHLKAAGKQNWAKLDAAIARIEAARAGGLRITANMYTYTAGSTGLDAAMPTWVQAGGR
                     EAWIRRLKDPATRRDVIAEMRVDSPAFENLYRFAGPEGTLLVGFQNPKLKPLTGKTLA
                     EVAKMRGVSPEDAAIDLVIEDGSRVQVIYFLMSEENVRKETALPWMSFGSDAAALAPE
                     GVFLQSSTHPRAYGNVARLLGKYVREERSLTLQEAVRRLSALPAHNLGLHDRGMLKAG
                     NFADVVIFDPATIADTATYESPKQFATGVRWVLVNGGVALKEGEPTALATGRVVRGRG
                     WKGWPDGGCRASAKDWTW"
     gene            complement(13746..>14296)
                     /locus_tag="PLO65_01350"
     CDS             complement(13746..>14296)
                     /locus_tag="PLO65_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26925.1"
                     /translation="FADAIATLAGPAAAAIIAVCAMLKAAGTLGGWILVTAEATRSGA
                     AAGFLPGALSSSSPARAPVRDLCLTALLMSLVIASSMSPTLGRQFGVLINIAVNLSMA
                     TYALCAVALLSFAGLIADPGRRLAARAMAIGGAGFSVWVVAASDPAMLAPSLWAFAAT
                     VPLYGLVLLARRRKGTLRKGTG"
BASE COUNT         2232 a         4888 c         4985 g         2191 t
ORIGIN      
        1 cgatcagggc caggccggtc aggccgagcg cgtgctgcgg gacgccgggc cgcaccctcg
       61 gcgcccacag ggccagcagc accccggcca gcagctccca ggcccgcgcc agcggcagat
      121 agaaggccgc gtccggatgg gcggcggtct gccacacggc cagcgccagg gagagcgcgc
      181 ccagtccggc caggaccagc ctgatccgtc ccgccgcgac cctcgcgagc agcagcatca
      241 gcaccgggaa gacgaggtag aactgctcct cgacgcccag cgaccaggtg tgcagcagcg
      301 gccggaccag gtcctcgccg ccgaaatagt cgctggtgcg ccagaaatgg acattggaca
      361 gcgacaccag cgccccggcc atcgaccgga cataggcgag caggtcgccg ggcgtcagaa
      421 gccaccaggc cacggcggtg gtcgccgcca gcatgaccat cagcgacggg aagatgcggc
      481 gcacccgccg ctcccagaac cagcccagcg agaagtcgcc gcgcgcctgc cgctcggcca
      541 ccacgccgcc gatcaggaag ccggagatga cgaagaacac gtcgacgccg gcgaacccgc
      601 cggtcagcca gggcgcgccc gcgtggaagc cgaccaccgc cagcaccgcg accgcccgca
      661 gcccgtcgac atccgccctg tatttcatgc gcccccggcc ctttcgagcg cggggcttta
      721 gcgcgcggtc atgccgccgt cgatgaccag ttccgcgccg gtcatgtagc gcgactcgtc
      781 ggaggccagg aacagcacac cggcggcgat gtcggccggg aagcccttga cgcccagcgg
      841 ggtcgcgacg gcggtcatgt catccaggtc gatggcgcgg gccggaccgg cgttgccgcc
      901 ctcgccgccg atcgtgccgc cgatggtgtc ccagatcggg gtctcgatga tgcccggatg
      961 gaccgagttg acccgaaccc cgtcgcgggc cgtggcgcac tccatggcca ccgccttggt
     1021 gaacagccgc acggccccct tggtggcgca gtagctggac atgttggccg ccccggtcag
     1081 gccggcgatc gacgacatgt tgatgatgct gccgccgccg ccctggcgca tcagcggcag
     1141 gctgtgcttc acgcccagga agacgccctc gacgttgacc ccctgctgct tgcgccagtc
     1201 ggccaggctg aactcggtga ccggcccgcc catgccgacc ccggcgttgt tgaccagcac
     1261 gtcgagccgg ccgaaacgct tgccgacctc ggtcaccacg ccgatccagg cgtcctcctc
     1321 ggtcacgtcg tgatgcaggt agacggcctt gccgccgagc gcccgggcga cgtccgcgcc
     1381 catgtcatcc tgcacgtcgg tgatcaccac ggcggcccct tcgcgcgcca gcagctcggc
     1441 gcagccgcga ccgatgccac tcgccccgcc cgtcaccagc acgaccttgc ccgcgacccg
     1501 acccgtcatt cctgcctccc ccggcttttt tgttatcacc gataaggtga cggcgagtcg
     1561 gggtggcgtc cagcagattg cgtccttcga cacggccctt cgggcctgct caggatgacg
     1621 tgcatttgcg ccgcctcatg gtgagcggcg ccgcaggcgc gtgtcgaacc atgcaggccc
     1681 taggcgtagg cgtacggccc gcccttctcc agcgccgccc ggtaggccgg gcgggcgtgg
     1741 attttttcga ggaaggccgt caggcggggt cggccgacca gggcgccggc gcggtcggcg
     1801 gcggcctcga cgggaaagct catcatgatg tcggcggcgc tgaagtcctt gccggcgaac
     1861 catccggctt tgccgagctc ggcctcccag tagtcgaaat gggctttcag ctgtggatcg
     1921 acgaagctct tcagggcctg gctggagatg cccctgacga tcccgcggat caggccaggc
     1981 gcgcgatcgg gcagggcggt gaacaccagt ttcatcagca acggcggcat ggccgagccc
     2041 tcggcgtagt gcagccagta gagccagcgc aggcggtcct cgctgccggc gggcggcttc
     2101 agcctgccac caccgtaggc ctcgacgatg gtctcgacga tggccccggt ctcggccagg
     2161 gtcgcgggcc cgtcggtgat caccggcgac ttgcccagcg ggtggatcgc cttcagctcc
     2221 ggcggcgcca gcatggtctt cgcgtcccgc tgatagtgcc tgatctcgta gggcagcccc
     2281 agctcctcca gcagccacag cacgcgctgc gagcgggagt tgttcaggtg gtggacggtc
     2341 agcatgggcg gaacctcggg gacatgcacc tctggtacgt gtcccccaat cgctccggct
     2401 tggatagggg acacgtacca gaggtacatg tccccatgcg ccgtcagcgc agctggccgg
     2461 ggtcgtagta gaatcgctcc tcgacgatcc ggtcgccgtc ccaccgctgc caggcgatct
     2521 cgtcgaggac acgggtggtt ccggcgtcat ccgtcatctc gaacacccag ttgatcgcca
     2581 cccggtcgcc gtcgaccagg acgacggaac ccttgcgggc gacgatgtcg ttacgggtca
     2641 gagcggcgcg ctccttctcc accagggccg cgcgaccgac gctcgttcgg ccgagatttt
     2701 cctgcgccgt ggcgcccgtg gcgtagaagg tctcgatggc ctcgacgaac cggccgtcct
     2761 cgaccatggc gacaaaggcg gcgacgcggg cgggcgtggg catcagcgat ctctttcaag
     2821 cgcggcgagc tcgacctcga tgaccgccag caggccggga agggactcct tgatcgtgtc
     2881 acgatctccc gagcgaacaa gatgttgtcg cggatgtgga gtaacgccgc ctccacgcta
     2941 gaaggcatgg atggcgtccc gttcggcgtt cgtcggatgt atttgttgat gccgagccga
     3001 ttgacgacgt cggtctttcg gcggccgaac agaccctgca catagcggat cactccggcg
     3061 tagtcccaca tggttatggg cgcgtccgga tcgaattcga ccatgatgtc gatgtcgctc
     3121 ttcggaccgg cgtcacccct cgccagagat ccgaacaggg cggcatggat cacgccctgc
     3181 ttgcgcagct cggcctcgtg atcgcgcaaa acctgaagga cgtcctcgcg ggtcatgggc
     3241 ggaaagatag catgtctcga cctcggggac atgtaccgaa ggtacgtgtc ccctgtccga
     3301 gcggctgggg tgggggacac gcacccaaga gggcgcatgt cccctaaaag ctctgcagcg
     3361 cccggacctc gagggccgcc agcggggcgc tggtgatggc ctgggcggcg gccagggcgc
     3421 cggctgacgt cgtgtagtag gggatcttca tcatcagggc ggtgcgccgc agctcgaagc
     3481 tgtcggccag cgactgcttg ccctcggtgg tgttgaacac cagctgcacc tcgccgttct
     3541 tcatcgcgtc gacgatgtgc ggccggcctt ccagcacctt gcggacatgc tcggtctcga
     3601 cgccctgcga ccgcagccat tcgccggtgc cggcggtggt cagcacccgg aagccggcgg
     3661 cctgcagcag cctgaccggc tcgatgatcc agatcttgtc gctgtccttg accgagacaa
     3721 aggccgtacc cgcggtcggc agggtcaccc cgccgccgag ctggctcttg gcgaaggcgc
     3781 gggcgaaggc gggggccatg tcggcttcgc cgtcgcgctt ccagtccagg cccatgacct
     3841 cgccggtcga gcgcatctcc ggacccagca ccgtgtcgac tccggcgaag cgggcgaacg
     3901 ggaagaccgc ctccttcacc gcgatgtggt cgtagggttt gtcgaccagg ccgaagctgg
     3961 ccagcgtttc gccggccatg atccgggccg cgatggcggc gacaggcgcg ccgatggtct
     4021 tggccacgaa cggcacggtg cggctggcgc gcggattgac ctccagcacg aagatgcgcg
     4081 gcgcggccga gtgcggctcc tcgatcgcga actgcacgtt catcaggccg cgcacgttca
     4141 gcgccagggc catctcgacg gtctggcgct tgagctcggc gacgatgtcc gggcgcagcg
     4201 agaagggcgg catcgagcag gcgctgtcgc ccgagtgcac gccggcctcc tcgatatgct
     4261 cgagcacgcc ggcgacgaac acgtcggtcc cgtcgcacag cgcgtcgatg tccacctcgg
     4321 tggcgcggct gaggtactgg tcgatcagga tcggcgcgtc gccgctgacc tcaacggcgg
     4381 tgcgcacgta gcgctcgagt tgctcgtcgt cgcggatgat ctccatgccc cgtccgccca
     4441 gcacatagga cggccggatg acgatcggat agccgacctc ggccgcggcg gtgaagcatt
     4501 cctcggcgct gcgggccagg gcgttgaccg gctggctgat gtccagccgg tgcagcagct
     4561 gctggaagcg ctcgcggtcc tcggccaggt cgatggcgtc ggggctggtg ccgaggatcg
     4621 ggatgccggc ctgctccagc ggatgggcca gcttcagcgg cgtctggccg ccgaactgga
     4681 cgatcacccc ggccagggtc ccggccttct gctcgacgtg gatcagctcc agcacgtcct
     4741 cggccgtcag cggttcgaag tacaggcggt cggaggtgtc gtagtcggtc gagacggtct
     4801 cggggttgca gttgaccatg atcgactcga tgccgatggc gtcgagcgcg aaggccgcgt
     4861 ggcagcagca gtagtcgaac tcgatgccct ggccgatgcg gttggggccg ccgccgagga
     4921 tgatcgcctt cttgcggctc gagggctcgg cctcgcagtc gggcgcctgg cccagggccc
     4981 cggtctcgta ggtcgagtac atgtagggcg tggtcgccgc gaactcgccg gcgcaggtgt
     5041 cgatgcgctt gaacacgggg cggatgttca tggcgtggcg acgggcccgg acctcgagct
     5101 cggtcaggct ggtcagcttc gccagccggg cgtcggagaa gcccatggcc ttcagtttcc
     5161 gcaggccgtc ggcggccggc aggccggagg cgcggacctt gccctcctgg cgcacgatct
     5221 cggcgatctg gcgcaggaac cacggctcat aggaacaggc gccgtggatc tcctcgaccg
     5281 tcaggccgtg gcggaaggcc tgggcgatga ccagcagccg ctcgggcgtc ggcatggcca
     5341 gggcccgcag caccgcggcg tgggcggtgt cggggtccga ggtgcccgga atatcgatct
     5401 cgtcgaggcc ggacaggccc gtctccagcc cgcgcagggc cttctgcagg ctttcggcga
     5461 aggtccggcc gatggccatg acctcgccga ccgacttcat cgaggtggtc aggtgcggtt
     5521 cgctgcccgg atacttctcg aacgcgaagc gcgggatctt ggtgacgatg tagtcgatgc
     5581 tcggctcgaa cgaggccggc atggcgccgc cggtgatgtc gttggccagc tcgtcgaggg
     5641 tgtagccgac ggccaggcgg gcggcgacct tggcgatcgg gaagccggtg gccttcgacg
     5701 ccagggccga cgagcgtgac acccgcgggt tcatctcgat gacgaccatc cggccatcct
     5761 tcggattgat cgcccactgc acgttggagc cgccggtctc gacgccgatc tcgcgcagca
     5821 cgtcgatgct gcccttgcgc atgcgctgat actctttgtc ggtcagcgtc agggccgggg
     5881 cgacggtgat cgagtcgccg gtgtggacgc ccatcgggtc cacgttctcg atcgagcaga
     5941 cgatgatgca gttgtccgcc ctgtcgcgga ccacctccat ctcatactcc ttccagccga
     6001 gcacactctc ctcgatcagc acctcggtgg tcggcgacag gtcgaggccg cgcagcacga
     6061 tctcctcgaa ctcctcgagg ttgtaggcga tgccgccgcc ggtgccggcg agggtgaagc
     6121 tgggccggat gatcgccggc aggccgacgt aggccaggcc ttcgcgggcc tcctcaaggg
     6181 tatgggcggc cttggagcgc gggctttcga ggcccagcct gtccatggcg tcgcggaatt
     6241 tctggcggtc ctcggccttg tcgatgacct cggccttcgc cccgatcatc tccacgccga
     6301 acttcgccag aacgcccatg gactccagcg acagagcggt gttcagcgcc gtctggcccc
     6361 ccatggtcgg cagcagggcg tcgggccgct ccttctcgat gatcttggcg accatttccg
     6421 gcgtgatcgg ctcgatgtag gtcgcgtcgg ccacgtccgg atcggtcatg atcgtggccg
     6481 ggttcgaatt gaccaggatg atgcggtagc cctcggcccg cagggccttg caggcctgga
     6541 cgccggaata gtcgaactcg caggcctggc cgatgacgat cggccccgcg ccgatgatca
     6601 ggatcgagga gatgtcggtc cgtttgggca tgctcgtctc gcgcacaagg gcggtcgcac
     6661 agaactgcgc gcccggcccc ggtcaaactg ctggttaagg ggcccgttta gagagggagt
     6721 cgggcgggtg caacccgctt tcccgctgtc atcccggccg cggggcggag ccccccggtc
     6781 gcgcgaagcg cgcccggggg aaaacgccga gcggggaccc ggattcggct tcatccacgg
     6841 tgaacgggat tcaatccggg tcccggatca gccctgcgga ctgtgcggga tgacagtgag
     6901 gagtcatcgc cagacacctc gggcgtgaga cccgcagcgg tcgtcaggtt cgatacgctt
     6961 gactcaatgt tatgtattat ttacatcatg tcgacattat cgaacctgag gggagcgacc
     7021 atgccgtatc gtgagaaatc caacctgttg gcgctggcgg cgatgctcat cgcctatgtg
     7081 ccctatttcg tggtcgcggc gatgacgccg gtccagaccg tggtgacgcc cgacctgctt
     7141 ctgctgaccc tgttcgccgc ggtctccgtc gcgcggttgc tgatcctcgg cgtgggctat
     7201 ctctggttcc gggtcacgac gccggacgat gtgcgcgagc cggccgacga gcgggaccgc
     7261 gcgatcaccc gccgatcgac caccgtcgcc tactacgtgc tgatggcggg cacgctcgtg
     7321 gcggccgccg tcatgccggt ctggttcgcg ggctggacca tcgtcaatgc cgccctgttc
     7381 gccatcgtcc tggcggaagt catccgctgc ctcgtggcgg ccgtcagcta ccggaggggc
     7441 tggaatggct gagggcgcga tcaccaacca gatccgcacc ctgcgcttcc tcgccgggga
     7501 gatgtcgcag gccgagctcg gccagaagat cggcgtcacc cgccagacca tcgcggcgat
     7561 cgaggccggc aagtactcgc cgaccctgga ggccgccttc cgcatcgccg aggtgttcgg
     7621 caagcccctg gacgaggtct tccagtggat tcccggcggt cagaggtagc tcagccacca
     7681 gtcgcggcgt cgccgcttga ggtcgcccca ccagcggcac aggggataga gcgatgccgt
     7741 cgcgacgatc cacaccccgt agaccaccgg cagcgggaag ccccagtggg cccggatcaa
     7801 cggcgaggga tcggccatga tccagacgaa ggtctgcgcc ggcagcccca tcgccagccc
     7861 ggtcagcagc atggccaggt gcagcaggta gatgtgggcg atgtagaaca tcagcggcgc
     7921 gcggccgtag acctcgagca cccgggccac cggtcccttc agccgctcca tcgccggcag
     7981 cagggccagc atcgagccca gggtgatcag caggtagtcc agcgacggcg ggtatttggt
     8041 cacgtcgaag aacgagaagc cccgccgcat cggctcgacc agcgccttcc acggcctggg
     8101 atcgccgtag gcgttgacgc cccgcaggac gaggaaggcg gcgatcatcg ccacccccag
     8161 ggcgatcagg gcccgggccc gccgccgcgg cgtccaggtc agcaccggcg ccaggccata
     8221 gccgagggcc atcaccccga tccacggcaa caccggatag acgatgaaga aggctgtcag
     8281 ggtcggcccg ccctcgtgca gcatgaccca cagcggcgcg gccgggccca ggtcagcggg
     8341 ccgcaccggg tccagcaggt tgtgcccggc gcagatcgcc acgccgatgg ccagcaccgc
     8401 gccgcgtggc agccagatca ggccggcaag ggcgatcatg ccgaccccca gggcccagat
     8461 cacctgcagg ccgaagccgt agatggtgaa gttccagccg tagttgagca gcaccatctc
     8521 gaggaagatc agccacaggc cgcgcgagac gaggaaccgc gccagcgccc cccggccctt
     8581 gccggcctgg ccctgcagga aggccgacat gccggccagc agcacgaagg tcggggcgca
     8641 gacatgggtc acccagcgcg tcaggtagac cgccaggctg gtcgccccgg cgtcggtcgg
     8701 atcgaacagg aaggcctggc tgtggaaata gtcgcgcgtg tgatccaggg ccatgatcac
     8761 gatgaccagg ccgcgcagca tgtcgaccga ggtcagcctg ggcggtctgg cgggcgtggc
     8821 catcgccccg tccatcgtcg tcgtggtcat cgcccgcccc cggcctgtcg cggcgcgatg
     8881 aaacgccgat ttgcgcgatc tgggtagtag gccggcgaac accggtctgc gtgattgacc
     8941 gccccgcgct ccgcccgtta cgactcgcgc gggaaacgaa acaaggggag gtctacatgc
     9001 gtattctgtt gggcgccgtc gcggcgctgg cgctctcggc cacggccgcc ctcgccggcg
     9061 gcgacgacat catggccacg cgcttcggca acaccaccat caccaccgac gccagcggcc
     9121 agtcctcaag gatctactac gaggccgacc acagcttcac cggcgtgcag ggcgaccagc
     9181 agctggccgg aacctgggaa gtgaagggca gccaggtctg cctgaccttc tcgagcgacg
     9241 cccctccggg ctaccccaac ccgatctgca ctgcggtgtc ggcccacaac gtcggcgaca
     9301 gctggaccag cggcccgttc acggtccagc tggtggcggg gcatcagtag gggagaaggg
     9361 gacatgtacc gcaggtacgt gtcccccaat cgagcggaac acgtttaggg gacacgtacc
     9421 caagagggta catgtcccct cgttctacgc cttcgccctg tccatcagcc ccgcgaaccg
     9481 ttcgaacaga tacaggctgt cggtcgggcc gggcgaggcc tcggggtggt gctggacgct
     9541 gaacacaggc ttgtccgcca gggccaggcc ggcgttggtg ccgtcgaaca ggctgacgtg
     9601 ggtctcggtg accggggccg gcaggctgtc gcggtcgacg gtgaagccgt ggttcatcga
     9661 gacgatctcg accttgccgg tcgtcaggtc cttcaccggg tggttcgccc cgtggtggcc
     9721 ctgctccatc ttgacggtcc tggcgccgag cgcgagggcc agcatctggt ggcccaggca
     9781 gatgccgaac accggcgtgc cgctgtcgac cagcttgcgg atctcgggca ccgcatattc
     9841 gcccgtcgcg gccgggtcgc ccgggccgtt ggacagcagc accccgtccg gcttgcgggc
     9901 caggatggcc tcggccgagg tgtcggccgg cacgaccgtc gcgcgggcgc cgatcgagct
     9961 cagcgcccgc aggatgttgc gcttcacgcc gtagtccaga accacgacct cgtacttcgg
    10021 cgcgtccggc ctggcgtagc cttccggcca gtcccacagg ccctcgtccc agacgaacga
    10081 ctggcgggtg gtggcgtcct tcgccaggtc gaggccttcc aggccggacc aggccctggc
    10141 cttcgccacc agggcgtcca ggtcgaagac gccgtccggg ctgtgggcga tgaccgcgtg
    10201 cggcatgccc ttctcgcgga tcgtgcgggt cagggcgcgg gtgtcgaccc cggccaggcc
    10261 gatcacaccc cggcgccgca tccagccggg caggtcgccg tcggcgcgcc agttggcggg
    10321 gtcggtcggc acatcccgga agatcgcgcc gcgcgccgcc gtctcgctgc cgccgctgat
    10381 ctgctccaga tcctcgacgt tggtgccgac gttgcccatg tgcgggaagg tgaaggcgac
    10441 gatctgggcc atgtaggagg gatcggtgag gatctcctgg tagccggtca tggcggtgtt
    10501 gaaacagacc tcgccgaccg cgtcgcccgc ggcgccgacg ccgatcccct gcaggatggt
    10561 cccgtcggcg agggccagaa cgccggtgac gccgggtaga acggtctgcg gcatgggcgg
    10621 ggttcctggc gggtggacaa acgggcgcgt tggtagtgag tcggacctgt tcggtcaaac
    10681 gctatcggag ggcttatgct ccagatgcgc cccaactgcg aatgctgcga ccgcgacctg
    10741 gcgcccaacg gcggcgacgc gcggatctgc agcttcgaat gcaccttctg cgccgactgc
    10801 gccctggacc ggttcgacgg tcgctgtccc aactgcggcg gcgacctggt cgtccgtccg
    10861 ccgcgcccgg cgaccctgct ggcgaagtat ccggccagca ccgagcgggt ggtgaaggat
    10921 catggggcgg cgacgtagtg cgtccttcga cacgccgctt cgcggctgct caggatgacg
    10981 tgcaaaggcg gacgtcatgg tgagcagcgc cgaaggcgcg tgtcgaacca cgcaatctcg
    11041 ctccgcccgc cttggcttgc cacgccacca gcgctagagc ccgccccatg tccatcacca
    11101 cggtcggcct cgatgccgac gacaccctct ggcacaacga gacgctgttc cgcctgacgc
    11161 accggcggtt caccgatctg ctcgcgccgc attgtgacga ggcgaccctc gaggcgcggc
    11221 tggccgagac ggagaagcgc aacctgcggc tctatggcta cggggtgaag ggtttcaccc
    11281 tgtcgatgat cgagacggcg atggaactga ccgacggggc cgtcgagacc gcggtgatcc
    11341 gcgagattct cgccgccggg cgcgagatgc tgaccgagcc ggtcgagccg ctgccaggcg
    11401 tcgacgcggc cctggcccag ctgtcggagc gctatcgcct ggtgttgatc accaagggcg
    11461 acctgctgca ccaggaacag aaactggcgg ccagcggcct gggcgacctg ttcgcggccg
    11521 tcgagatcgt cagcgagaag gacgccgaca cctatcgccg ggtgttcgcc cgccacggca
    11581 cggggccgga acaggcggtg atggccggca actcgatgcg gtccgacatc ctgccggcgc
    11641 tcgacgccgg ctgctggggc gcgctgatcc cctatccgct ggtctgggcc cacgaggcgg
    11701 ccgaggctcc ggccgatcat gatcgctacg tcgagttggc ctcgatcagc gatctgccgg
    11761 ggtgggtgga cggggtggcg gggtagggca ttcgtccttc gacacggccc gttgggcctg
    11821 ctcaggatga cgtgtaatcg tgtacgtcat ggtgagcagc gcccgcaggg cgcgtgtcga
    11881 accacgcagg gagtatccgc cttgagactt gccccgttca ccgccctgct gagcgccgtc
    11941 ctgctcctgt cctggtcctc gacctccacc gcggccttgc cgtgggcccg gtcgaaggcg
    12001 gtcccgttcg acatcgtcat ccgcaacgga accgtctacg acggctcggg cggggcgccg
    12061 ttcgtcggcg acgtggccat tcgcggcgac cggatcgtcg cggtcggcaa gaagctcagg
    12121 ggcccgcgcc tgaccgagat cgacgccacg ggcctggccg tctcgccggg cttcatcaac
    12181 atgctcagct gggccaccga gagccttctc gccgacggcc ggggcctgtc cgatctcaag
    12241 cagggcgtga ccctggaggt gatgggcgag ggctcctcga tgggcccgtg gaacgaaaag
    12301 atgcgccggc aggagcttgc gcggcagggc gacatcaagt tcgacatcag ctggaccacc
    12361 ctggacggct acctgagcca cctggagacc ctgggcatcg cccagaatgt cgcctcgttc
    12421 gtcggcgccg agacggtgcg caccaacatc ctcggcgagg aggacgtcga cccgaccccg
    12481 gccgagctgg cgcggatgcg ggcgctggtc gtccaggcca tggaggaggg cgcgctcggc
    12541 gtcggctcgt cgctgatcta cgccccgggc agctacgccg acaccgacga gttggtcgcc
    12601 ctgtcgaccg aggccggccg ctgcggcggc atgtacatca gccacatgcg cagcgagggc
    12661 aaccggctgc tggaggcgat cgacgagctg atcgacatct cgcgccgctc gggcgcgccg
    12721 gccgagatct accacctcaa ggcggccggg aagcagaact gggccaagct cgacgcggcc
    12781 atcgccagga tcgaggcggc ccgcgccggc ggcctgcgca tcacggccaa catgtacacc
    12841 tatacggccg gttcgaccgg actggacgcg gccatgccga cctgggtcca ggccggcggg
    12901 cgcgaggcct ggatccggcg gctgaaggac ccggccaccc gccgcgacgt gatcgccgag
    12961 atgcgcgtcg acagcccggc gttcgagaac ctctaccgct tcgcggggcc ggaggggacg
    13021 ctgctggtcg gtttccagaa tccgaagctc aagccgctga ccggcaagac cctggccgag
    13081 gtggcgaaga tgcgcggggt cagccccgag gacgcggcca tcgacctggt catcgaggac
    13141 ggcagccgcg tgcaggtgat ctatttcctg atgagcgagg agaacgttcg caaggagacg
    13201 gccctgccgt ggatgagctt cggctccgat gccgccgccc tggcgcccga gggggtcttc
    13261 ctgcagtcga gcacccatcc gcgcgcctac ggcaatgtgg cccggctgct gggcaagtac
    13321 gtccgcgagg agcgcagcct gacgctgcag gaggccgtgc ggcggctgtc ggccctgccg
    13381 gcgcacaatc tgggcctgca tgaccggggg atgctgaagg ccgggaactt cgccgacgtg
    13441 gtgatcttcg acccggcgac catcgccgac actgccacct acgagtcgcc gaagcagttc
    13501 gcgaccggcg tgcgctgggt tctggtcaac ggcggcgtgg cgctgaagga gggtgagccg
    13561 acggccctgg ccaccggacg cgtggtccgc ggccgcggct ggaagggctg gcccgacggc
    13621 ggctgccgcg ccagcgcgaa ggactggacc tggtagggtc ggtgcgtcct tcgagacgcg
    13681 atcctgaggg atcgctcctg ggaatggggg gaatggggac aggttaaact gtccctcggg
    13741 actgtttaac ctgtcccctt acgcagtgtc cccttacgcc gcctcgccag cagcaccagc
    13801 ccatacagcg ggaccgtcgc ggcgaaagcc cacaggctgg gcgccagcat ggccgggtcc
    13861 gaggcggcca cgacccacac cgaaaagccc gcgccgccga tcgccatggc tcgggcggcc
    13921 aggcgcctgc cgggatccgc gatcaggccg gcgaagctga gcagcgccac ggcgcaaagg
    13981 gcgtaggtgg ccatgctcag gttcacggcg atgttgatca acacgccgaa ctgcctgccc
    14041 agggttggcg acatgctcga cgcgatcacc agcgacatca gcagggcggt gaggcacagg
    14101 tcgcgcacgg gcgcgcgggc cggactcgaa ctggacaggg cgcccggcag aaaacctgcc
    14161 gcggcgccgc tccgcgtggc ctccgcggtc accaggatcc agccgcccag ggtgccggcc
    14221 gccttgagca tggcgcagac ggcgatgatc gcggcggcgg ccggaccggc gagggtcgca
    14281 atcgcgtcgg cgaacg
//
LOCUS       DAORNX010000016        14223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3234_length_14223_cov_6.811688, whole genome shotgun sequence.
ACCESSION   DAORNX010000016 DAORNX010000000
VERSION     DAORNX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_14223_cov_6.811688"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>208)
                     /locus_tag="PLO65_01355"
     CDS             complement(<1..>208)
                     /locus_tag="PLO65_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26926.1"
                     /translation="ASAGTDNTLFRLGDELLVRLPRVDWAAGMIAKEQRWLPTFSHRL
                     PLAISEPMGLGEPAEGYPWHWGVYA"
     assembly_gap    209..389
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..1244)
                     /locus_tag="PLO65_01360"
     CDS             complement(<390..1244)
                     /locus_tag="PLO65_01360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRD26927.1"
                     /translation="MTDLAATSRETIQKGSQSFAAAAALFDPQTRADAEMLYAWCRHC
                     DDVIDGQALGHDREMVDQAEVEQRLVDLYARTRAALAGEPTDDPAFAAFREVARRRAI
                     PERYPIALIDGFAMDVEGRRYETIEDTLDYCWHVAGVVGVMMAIVMGVKPDDLATLRR
                     AQDLGLAFQLTNIARDIVDDAQNDRVYLPADWLAAGGVPETALAAPEHRAAIAGMARR
                     LVELAEPYYASARWGLRGLPFRSAWAVAAALGVYREIGVKVVARGAKAWDQRVSTSTG
                     EKVKLALGG"
     gene            complement(1241..2728)
                     /locus_tag="PLO65_01365"
     CDS             complement(1241..2728)
                     /locus_tag="PLO65_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HRD26928.1"
                     /translation="MTRPRAAVIGSGFGGLALAVRLQSAGFDTTIFEARDKPGGRAYV
                     WHEEGFTFDAGPTVITDPDCLEQLFALSGRRLADYVDLVSVDPFYRLCWEDGQTFDYV
                     NDQARLDAQIAARNPADVEGYKRFLAYSEALFQEGYVKLGHVPFLDFKSMLAAAPALM
                     KLQAWRSVYARVATFVQDEHLRQALSFHTLLVGGSPFATSSIYALIHALERRGGVWFP
                     KGGTHRLVTALATLFQDLGGTLRLSTPVRRITTRAGRVSGVELADGAVEAFDTVASNA
                     DIVHTYEKLLGGEPRGRSEAKRLKKARFSPSLFVVHFGLRAEHPQMRHHTICFGARYR
                     ELIGEIYGKNSLAKDFSLYVHAPCASDPSMAPAGCSSFYALSPVPHLGTADIDWAVEG
                     PKYRDRILAYLEARYLPNLTRDLVVSRIFTPADFQRELNAWQGSAFSLEPILTQSAWF
                     RTHNRDDTIPNLYFVGAGAHPGAGIPGVVGSARATAGLMIEDFLP"
     gene            complement(2725..3906)
                     /gene="crtY"
                     /locus_tag="PLO65_01370"
     CDS             complement(2725..3906)
                     /gene="crtY"
                     /locus_tag="PLO65_01370"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HRD26929.1"
                     /translation="MASPNAFRADVVLVGGGLANGLIALRLRALRPELRVILLEGETT
                     FGGRHTWSHFASDVSPEIGSWLAPLIAHRWDGYEVRFPTHRRVLSTPYLAAPSERFHA
                     VLSAALGEDGWTGAAVARLDAGAVLLTDGRRVTADCVIDGRGPAPAPSLALGWQKFLG
                     LEVELTAPHGLTRPIVMDATVDQLDGYRFLYCLPFSATRLLIEDTRYSDGHGLDRDAL
                     AAAIEAYAASQGWTIDSVVRQETGILPIALAGDIDAYWRAADTARVGLRAALFHPVTG
                     YSLPDAARLADEIAALPVLSTATVRACVEARSKQRWRERAYYRLLNRMLFRAAEPGRR
                     YRVLERFYRLRQDLVERFYAGRITLADKARVLIGKPPVPIAAALAVLSEQSVLSGDLQ
                     T"
     gene            complement(3938..4435)
                     /locus_tag="PLO65_01375"
     CDS             complement(3938..4435)
                     /locus_tag="PLO65_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HRD26930.1"
                     /translation="MSLNRLFTRFANAVSKWTGRPVAFTLCVIGVLAWAVSGPAFGFS
                     ETWQLVINTSTTIVTFLMVFLIQNTQNRDNAALHAKLDELIRVGASDNRFIGIEHLTD
                     QELEVILEECEKAAKELHVELRNKRSHHKDAGAAKAVEVPAIVKKAVAKRSAARKPAA
                     KRKPA"
     gene            4506..5408
                     /gene="folD"
                     /locus_tag="PLO65_01380"
     CDS             4506..5408
                     /gene="folD"
                     /locus_tag="PLO65_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRD26931.1"
                     /translation="MAEATATIIDGKAFAARLYERVAGDVATLKAEHGVTPGLAVVLV
                     GEDPASEVYVRNKGLQSLAAGMHSETHKLPADASEADVLALVAKLNADPAIHGLLVQF
                     PVPDHLSQARIVAAISPDKDVDGLTVINAGRLASGLPALTSCTPVGCMMLLRDALGDL
                     TGKHAVVIGRSNLMGKPMAQLLLAADCTVTIAHSRSRDLPAICRQADILVAAVGRAEM
                     VRGDWIKPGAAVIDVGITRFPSRDPERAAAGKTRLVGDVAFDEAKTVAGWITPVPGGV
                     GPMTIACLLLNTLTAARRLHGLEG"
     gene            5419..5769
                     /locus_tag="PLO65_01385"
     CDS             5419..5769
                     /locus_tag="PLO65_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRD26932.1"
                     /translation="MAVILALGLAAIACSPKAAPAPTPEQALLLRPSDPRLSDLYATS
                     CMACHAIPESGAPMVHDAAAWAPRWKQGLPVLVDHSIQGFNAMPAGGQCATCTPADLE
                     ALIRFMADHEGSAG"
     gene            5785..7188
                     /locus_tag="PLO65_01390"
     CDS             5785..7188
                     /locus_tag="PLO65_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HRD26933.1"
                     /translation="MISRRQALVAGGAGIAVVAGSAVAVGVVSHDRPEPPAPPSTDGK
                     GHTVWRNWSGIQHSYPVQRSAPKDEAELAALLKTGPTPLRVTGAGHSFTGLCASDGTL
                     VSLDAMNGIERWDGDEATVRAGTRLGALGPALAAKGRAMANLPDINKQSLGGALGTAT
                     HGTGRTLKAIHGDVTALTLVTAGGEVINCDARVRPEVFDAARVSLGSLGVIAKARLST
                     TANRRLRRRVWIEPFEAALEQAEARWRTHRNFEFYAVPFTGLAANIAHDETDEAATER
                     PPSQDDAFLDALKGLRNLLGFSTGLRKAAAKALLGGTKPETAVDEGWKLLSTERPVRF
                     NEMEYHLPAETQLAALREVVAVIEKHRPDVFFPIEVRRIAPDDAWLSPFQGEPRGSVA
                     VHAYYRDDYAFFFALIEPIFRRHGGRPHWGKLHSLKGADLAALYPRWNDFLAVRRELD
                     PQGRLLNPYLKTLFGVS"
     gene            complement(7220..7837)
                     /locus_tag="PLO65_01395"
     CDS             complement(7220..7837)
                     /locus_tag="PLO65_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRD26934.1"
                     /translation="MSRRLTAAALALAAIALSGCISVFPKAEPVQLYRFEAHAVAPPT
                     AQGPVFGVLRLGVGFPRASAGDRILTVSDRGEAAYIAGARWVSPASVLFEEQLASAFQ
                     GGRARLIGRGEVVKADVSLKLDVQTFEARYDRGPKAAPEVVVSVRGVITRNRDRALVG
                     ERIFNARVRAGENRVSAIVPAFNAALTQVLGEISGWVNTVDPAGT"
     gene            complement(7834..8772)
                     /locus_tag="PLO65_01400"
     CDS             complement(7834..8772)
                     /locus_tag="PLO65_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRD26935.1"
                     /translation="MERNANYALVGVASLVLFVGLVVFVFWLARLQFNQDYDIYDVLF
                     VGPVNGLSEGGEVHFNGIKVGEVTKISLDPRDSRRVIARTRMSSDVPVREDSVGTLEP
                     QGITGVNFIQITAGTASKRLLRDTYPPGEVPVIRTQRSALSDLLQGGGTVLSRSIEAL
                     DRVNRVLSDENVKTFSAALSDVQAVTAEARERKEIIADAQKALQSIDAAATEITELSR
                     TTRGLVDGDGRRTLAELGDAATELKAATRDARAMIGKLDGPTSDFADTGLPQLTRAIT
                     SLQATAESLDRLVGEVEQNPRGVVSKAPAREVEVQP"
     gene            complement(8772..9584)
                     /locus_tag="PLO65_01405"
     CDS             complement(8772..9584)
                     /locus_tag="PLO65_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD26936.1"
                     /translation="MIGPDDLEAPEAPPIEIIGLRSQFGDHVIHDDLDLTVQRGEILG
                     VVGGSGSGKSVLMNTIIGLKQPDGGVVRLFGHDIDTATRKNWTSIERRWGVLFQQGAL
                     FSSLTVRENVAAPLHEHTRLSRAEINELADLKIALSGLPPRAGALKPAELSGGMIKRA
                     GLARALALDPQLLFLDEPTSGLDPIGAAAFDDLIADLSSSLNLTVFMITHDLDSLYAV
                     TTRVAVLADRKVVASAPVRELEHSDHPWIQEYFLGPRGRAAAGSRHRTGHDV"
     gene            complement(9581..10696)
                     /locus_tag="PLO65_01410"
     CDS             complement(9581..10696)
                     /locus_tag="PLO65_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRD26937.1"
                     /translation="MSKPADFKLQEGDQNARVVLTGDWTAESLDDADNRLAGILDGSR
                     SVTIDLMNMRRCDTAGAYAILRAAEGQLTAGQIVARRETRRLLDLVAAAVEAEPTPPT
                     RRHGVFDLFERIGKGVFNIVHEGAETFAFIGRLTMSVLRGLANPRRMRWAATFAQCER
                     AGLDALPIVATGTAFIGAVVALIGINTLSDFGATVFVVETIGIAVLREFGIVITAVLI
                     AGRSASAFAAEIGAMKMRQEIDAMQVMGVDPFDALVLPRFLALLVMMPLLTFVADISG
                     MVGGMLITWNILDLSPTFFLQRIADNVGATHFWVGISKAPIMAAVIAGIGSRQGMEVG
                     GDVISLGQRVTSAVVQAIFTIIVIDAIFAVIYMELDI"
     gene            10837..12588
                     /locus_tag="PLO65_01415"
     CDS             10837..12588
                     /locus_tag="PLO65_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HRD26938.1"
                     /translation="MTTGIDGQADFRTTGWTRIATGLAQGLALWALTEIVDGKAGTTF
                     NHVMLGALEAVALFTPFIVLGGLAEMRRRTLVIWTVGATVALAGLGAHDIGRRVAETA
                     PQWLSGEAMVFFAAVLFIAHHLVAGAEADRRPIARFATYFDLAWKHGVQLAMAAGFVG
                     VFWIVLFLGGALFKLIGIGFVEELIRKSWFAIPATATMFAAAVQLTDVRHALIRGVRT
                     VALTLLSWLLPVLVFLTGAFMAALPFTGLEPLWATKSAAAILLSAAAVMIILINAAYQ
                     DGEPDEPTPVVLRWAARAGGLLLVPLVAVAAYAVWLRVNQYGLTPDRIIAGACVIAGV
                     CYAVGYGVAALLPGAWMKRLEVTNVTTALVVVALILALFSPVADPARLSVNDQMARLA
                     AGKVAPDKVDYRFLRFESGRYGMEALATLKARGGDIGRRATEALALKERYRRDVAEQP
                     RPRDMASQVTVWPRDRTLPDSFINRRWDDDPRSPVRCDGKDDKCEATFADVNGDGREE
                     ILLAFSGAVTVYEEGTPGVWRVVGWTDSLNCDGDGVAVGEGRVKVAPRPWADLLVNGR
                     RVPLNVEPAGCPEPASH"
     gene            complement(12658..12732)
                     /locus_tag="PLO65_01420"
     tRNA            complement(12658..12732)
                     /locus_tag="PLO65_01420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12698..12700),aa:Val,seq:cac)
     gene            complement(12764..13480)
                     /gene="gph"
                     /locus_tag="PLO65_01425"
     CDS             complement(12764..13480)
                     /gene="gph"
                     /locus_tag="PLO65_01425"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662626.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRD26939.1"
                     /translation="MHDPSKPLAGWTVAFDLDGTLVETAHDLVGALNVILVEQGMAPA
                     PMTAVRQLVGHGLRGMLIRAFAMADLTITEEQIAQLRPRIVDVYRARIARESRPFPGC
                     LEALAALRDSGAALAVCTNKPEGLARLLLDELDMTRLFDAIVGGDTLPVQKPDPAPLL
                     AAIARAGGDRDHAVLVGDASPDVGAAKAAGVPCVVCSFGYNDLPPDELGGDVSIDHFD
                     ALARAVADIAASCRTAAESL"
     gene            13535..>13834
                     /locus_tag="PLO65_01430"
     CDS             13535..>13834
                     /locus_tag="PLO65_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRD26940.1"
                     /translation="MSQRAAVIMAAGQGTRMKSPTPKVLHRIGGRTMLDRAIDAVQAA
                     GCGRIVVVCGTHSPAVAEHVRKRLGEGAVAIQDPPLGTAHAVLAAKAALAGFAGDV"
     assembly_gap    13835..14002
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14003..>14223
                     /gene="glmU"
                     /locus_tag="PLO65_01435"
     CDS             <14003..>14223
                     /gene="glmU"
                     /locus_tag="PLO65_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRD26941.1"
                     /translation="RIVEARDATAEEKAVRACYSGILCAPAGPLFDWLAAVDNNNAKA
                     EYYITSIVGIANAAGKRVGVAFTSETAVM"
BASE COUNT         2114 a         4742 c         4971 g         2047 t
ORIGIN      
        1 cgcgtagacg ccccaatgcc agggatagcc ctcggccggc tcgcccagcc ccatgggttc
       61 ggagatggcc agcggcaagc ggtgcgagaa cgtcggcagc caacgctgct ccttggcgat
      121 catgcccgcc gcccagtcca cgcgcggcag ccgcaccagc aactcgtcac cgagccggaa
      181 cagggtgttg tccgtgccgg ccgaggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccgcccagcg ccagcttcac cttctccccc
      421 gtgctggtgg acacgcgctg gtcccaggcc ttggcgccgc gcgccacgac cttcacgccg
      481 atctcgcgat agacgcccag cgccgccgcc actgcccagg ccgagcggaa gggcagacca
      541 cgcaggcccc atcgggcgga ggcgtagtag ggctcggcaa gctcgaccag ccgccgggcc
      601 atgccggcga tggcggcgcg atgctccggc gcggccaggg ccgtctccgg cacgccgccc
      661 gccgccagcc agtcggccgg gaggtagacc cggtcattct gcgcgtcatc gacgatgtcg
      721 cgggcgatgt tggtcagctg gaaggccagc ccaaggtcct gggcgcgccg cagggtggcc
      781 aggtcgtcgg gcttcacgcc catgacgatg gccatcatca ccccgaccac gccggcgaca
      841 tgccagcagt agtcgagcgt gtcctcgatc gtctcgtagc ggcggccttc cacatccatg
      901 gcgaagccgt cgatcagcgc gatcgggtag cgctcgggaa tggcccgccg gcgggcgacc
      961 tcgcgaaagg cggcgaaggc cggatcgtcg gtcggttcgc ccgccagggc cgcccgcgtc
     1021 cgggcgtaca gatcgaccag ccgctgctcg acctcggcct ggtcgaccat ctcgcggtcg
     1081 tgacccagcg cctggccgtc gatcacgtcg tcacaatgac ggcaccaggc gtagagcatc
     1141 tcggcgtcgg cccgggtctg cggatcgaac agggcggcgg ccgcggcgaa gctctgcgac
     1201 cccttctgga tcgtctcgcg gctggtcgcg gccaggtcgg tcacggcagg aagtcctcga
     1261 tcatcagccc cgccgtcgcc ctcgccgagc cgaccacgcc aggaatgccc gcgccgggat
     1321 gggcgcccgc cccgacgaaa tacaggttcg ggatcgtgtc gtcgcggttg tgggtgcgga
     1381 accaggcgct ctgggtcagg attggctcga gcgagaaggc cgagccctgc caggcgttga
     1441 gctcgcgctg gaagtcggcc ggggtgaaga tgcggctcac gacgaggtcg cgcgtcaggt
     1501 tcggcaggta acgcgcctcg agataggcca ggatgcggtc gcggtatttc ggcccctcga
     1561 cggcccagtc gatgtccgcg gtgcccaggt gcggcacggg cgacagggcg tagaagctcg
     1621 agcagccggc cggcgccatc gagggatcgc tggcgcaggg cgcgtgaaca tagagcgaga
     1681 agtctttggc caggctgttc tttccgtaga tctctccgat cagctcgcga tagcgggcgc
     1741 cgaaacagat ggtatggtgg cgcatctgtg ggtgctcggc cctgaggccg aagtgcacga
     1801 cgaacaagga ggggctgaac cgcgccttct tcaggcgttt cgcctcggac cgcccgcgtg
     1861 gctcgccgcc gagcagcttt tcgtaggtat ggacgatgtc ggcgttggag gcgacggtgt
     1921 cgaacgcctc gaccgcgccg tcggccagtt caacgccgct gacccgcccg gcccgggtcg
     1981 tgatgcgcct caccggcgtc gacagccgca gcgttccgcc caggtcctgg aacagggtcg
     2041 ccagggcggt gaccagcctg tgcgtcccgc ccttggggaa ccacacgccg ccgcgccgct
     2101 caagcgcatg gatcagggcg tagatcgagg aggtggcgaa cggactgccg ccgaccagaa
     2161 gggtgtggaa cgacagcgcc tgccggagat gctcgtcctg cacgaaggtc gccaccctgg
     2221 cgtagacgct gcgccaggcc tgcagcttca tcagcgccgg cgcggccgcg agcatgctct
     2281 tgaagtcgag gaagggcaca tggcccagct tgacgtagcc ctcctggaac agcgcctcgg
     2341 aataggcgag gaagcgcttg taaccctcga catcagcggg gtttcttgcc gcgatctggg
     2401 cgtcgagcct ggcctggtcg ttgacatagt cgaaggtctg gccgtcttcc cagcacagcc
     2461 ggtagaaggg atccacgctg acgagatcga cgtagtccgc gagccggcgg cctgacaggg
     2521 cgaacagctg ctccaggcag tccggatcgg tgatcacggt cgggccggcg tcgaaggtga
     2581 agccctcctc gtgccagacg taggcccggc cgccgggctt gtcgcgagcc tcgaagatgg
     2641 tggtgtcgaa gccggcggac tgcagccgga ccgccagggc cagtccgccg aagcccgacc
     2701 cgatcaccgc cgcgcgcggc ctggtcatgt ttggaggtct ccggaaagga cagactgttc
     2761 ggacagcacg gccagcgcgg ccgcgatcgg caccgggggc ttgccgatca gcacgcgggc
     2821 cttgtcggcc agcgtgatcc ggccggcgta gaagcgctcc accaggtcct gccgcaggcg
     2881 gtagaagcgt tcgagcaccc ggtatcgccg acccggctcc gccgcgcgga acagcatgcg
     2941 gttgagcagc cggtagtagg cccgttcgcg ccagcgctgt ttcgagcgcg cctcgacaca
     3001 ggccctcacc gtggcggtgg acaggacggg cagggcggcg atctcgtcgg ccaggcgcgc
     3061 cgcgtcgggc agggaatagc cggtcaccgg atgaaacagg gccgcgcgca ggcccacccg
     3121 ggcggtgtcc gcggcccgcc agtaggcgtc gatgtcgccg gccagggcga tgggcaggat
     3181 gccggtctcc tggcgaacca cggagtcgat tgtccagccc tggctggcgg cataggcctc
     3241 gatcgccgcg gccagcgcgt cccggtccag tccgtggccg tcgctgtaac gggtgtcctc
     3301 gatcagcagt cgcgtggcgg agaagggcag gcagtagagg aagcggtagc cgtccagctg
     3361 gtcgacggtg gcatccatga cgatcggccg cgtcaggccg tgcggcgcgg tcagctcgac
     3421 ctcaagcccg agaaacttct gccagccgag cgccagcgac ggggcgggcg ccgggccccg
     3481 gccgtcaatc acgcagtctg cggtgacacg gcggccgtcg gtgagaagca ccgctccggc
     3541 gtccagccgg gcgaccgccg cccccgtcca gccatcctcg ccgagcgccg cggacagcac
     3601 ggcatggaaa cgctctgagg gcgcggccag atacggcgtg ctcaggaccc gccgatgggt
     3661 cggaaaccgc acctcgtagc catcccagcg atgggcgatc agcggggcca gccagctacc
     3721 gatctccggg ctcacatcgc tggcgaaatg gctccaggtg tgccgtccgc cgaaggtcgt
     3781 ctcgccctcc agcaggatga cccgcagctc gggtcgcagg gcccgcagcc gcagggcgat
     3841 caggccgttg gccagcccgc cgcccacgag gacgacatcg gcgcggaatg cgttgggcga
     3901 ggccatgccc cggagatagc acgccccccg tcgaaggcta ggcgggcttg cgtttggccg
     3961 ccggtttgcg ggcggctgac cgtttcgcca cggccttctt cacgatcgcg ggcacctcga
     4021 ccgccttggc cgccccggcg tccttgtggt ggctgcgctt gttgcgcagc tcgacatgca
     4081 gctccttcgc ggccttctcg cactcctcga ggatcacctc gagctcctgg tccgtcagat
     4141 gctcgatgcc gatgaacctg ttgtccgagg cgccgacgcg gatcagttca tcgagcttgg
     4201 cgtgcagggc cgcattgtcg cggttctggg tgttctggat caggaacacc atcaggaagg
     4261 tgacgatggt ggtcgaggtg ttgatcacca gctgccaggt ctcggagaag ccgaacgcgg
     4321 gaccggacac ggcccacgcc agcacgccga tgacgcagag ggtgaaggcg accggccgtc
     4381 cggtccattt cgacacggcg ttggcgaacc gggtgaacag cctgttgagc gacatggcgg
     4441 acctgtgatt gcgcggcgcg cggtgaacgg gtagcagcct cgcaggttcc cccggggaga
     4501 caggcatggc cgaggcgacg gcgaccatca tcgacggcaa ggcgttcgcg gccagactct
     4561 atgagcgcgt cgccggggac gtcgccacgc tgaaggccga acacggcgtc accccgggcc
     4621 tggccgtggt gctggtcggc gaggatccgg ccagcgaggt ctatgtccgc aacaagggcc
     4681 tgcagtccct ggccgccggc atgcattcgg agacccacaa gctgccggcc gacgcgagcg
     4741 aagccgacgt gctggccctg gtggcgaaac tcaacgccga cccggcgatc cacggcctgc
     4801 tggtgcagtt cccggtgccc gaccacctca gccaggcgcg catcgtcgcg gccatctcgc
     4861 cggacaagga cgtcgacggg ctgacggtga tcaacgccgg acggctggcc agcggcctgc
     4921 cggccctgac ctcctgcacg ccggtcggct gcatgatgtt gctgcgggac gccctcggcg
     4981 acctgaccgg aaagcacgcg gtggtcatcg gccgatccaa cctgatgggc aagccgatgg
     5041 cccagctgct gctggccgcc gactgcacgg tgaccatcgc ccacagccgc agccgggacc
     5101 tgccggcgat ctgccgccag gcggatatcc tggtggccgc cgtggggcgc gcggagatgg
     5161 tccgcggcga ctggatcaag cccggagcgg cggtcatcga cgtgggcatc acccgcttcc
     5221 cctctcgcga tccggaacgg gcggcggcgg gcaagacccg gctggtcggc gacgtggcgt
     5281 tcgacgaggc gaagactgtc gcgggctgga tcacgccggt cccgggcggg gtcggaccaa
     5341 tgaccatcgc ctgcctgctg ctgaacaccc tgaccgccgc gcggcggctg catggcctgg
     5401 agggctgaga tgggacgaat ggctgtcatc ctggcgctgg gccttgcggc gatcgcctgt
     5461 tcgcccaagg cggctcccgc gccgactccc gagcaggccc tgttgctgcg gccgtccgac
     5521 ccgcggctgt cggacctgta cgccacctcc tgcatggcct gccacgcgat ccccgaatca
     5581 ggggcgccga tggtccatga cgccgccgcc tgggccccgc gctggaaaca gggccttccc
     5641 gtgcttgtcg accacagcat ccaggggttc aacgccatgc cggccggcgg acagtgcgcg
     5701 acttgcacgc cggccgatct ggaggctctc atacgattca tggcggacca tgagggttcc
     5761 gccggctagg ggcggggacg ggtcatgatt tcgaggcggc aggcgcttgt cgcgggcggg
     5821 gcgggtatcg ccgtggtcgc cggttcggcc gtcgcggtgg gggtcgtcag tcatgaccga
     5881 ccagagcccc cggccccccc gtcgaccgac ggcaagggcc atacggtctg gcgcaactgg
     5941 tcgggcatcc agcacagcta cccggtccag cgcagcgccc cgaaggacga ggccgaactg
     6001 gcggccctgc tcaagacggg ccccacgccg ctgcgcgtga ccggcgccgg tcacagtttc
     6061 accggcctgt gcgccagcga cgggacgctc gtctcgctgg acgccatgaa cggcatcgag
     6121 cggtgggacg gcgacgaggc gacggtccgg gccggcacgc ggctcggggc gctgggcccg
     6181 gcgctggccg cgaagggccg ggcgatggcc aacctgcccg atatcaacaa gcagtcgctc
     6241 ggcggcgccc tgggcacggc gacccacggg acggggcgga cgctgaaggc gatccatggc
     6301 gacgtcacgg ccctgaccct ggtcaccgcc ggcggggagg tgatcaactg cgacgcccgc
     6361 gtccggccgg aggtgttcga cgccgcgcgc gtctcactgg ggtccctcgg ggtgatcgcg
     6421 aaggcccggc tgtcgaccac cgccaaccgc cgcctgcgtc gccgggtgtg gatcgagccg
     6481 ttcgaggcgg cgctggagca ggccgaggcg cgctggcgga cgcaccgcaa cttcgagttc
     6541 tacgccgtgc ccttcaccgg gctcgcggcc aacatcgctc acgacgagac cgatgaggcg
     6601 gccacggaac ggccgcccag ccaggacgac gccttcctcg acgcgctcaa ggggctgcgc
     6661 aacctgctcg gcttctccac cgggctgcgc aaggcggcgg cgaaggcgct gctcggtggg
     6721 acaaagcccg agacggcggt cgacgagggc tggaagctgc tgtcgaccga gcggccggta
     6781 cggttcaatg aaatggaata ccacctgccg gcggagaccc agctggccgc gctcagggag
     6841 gtggtcgcgg tcatcgaaaa gcaccggccg gacgtcttct tccccatcga ggtgcgccgc
     6901 atcgcccccg acgacgcctg gctgtcgccc ttccagggcg agccgcgcgg gtcggtggcc
     6961 gtccacgcct actaccgcga cgactacgcc ttcttcttcg cgctgatcga accgatcttc
     7021 cggcgacatg gcggccggcc ccactggggc aagctgcatt cgctgaaggg agcggacctg
     7081 gcagccctct acccgcgctg gaatgatttc ctggccgtgc gccgtgaact cgacccccag
     7141 ggccggctgc tcaatccgta cctgaagacc ttgttcgggg tgagctagga caccttctcc
     7201 cgcaggagga gacggcctct tacgtccccg ccggatccac cgtgttgacc cagcccgaga
     7261 tctcgccgag cacctgggtg agggcggcgt tgaacgccgg tacgatcgcg ctgacacggt
     7321 tctcgccggc gcgcacgcgg gcgttgaaga tccgctcgcc gaccagcgcc cggtcgcggt
     7381 tgcgggtgat cacgccccgt acggacacca ccacctccgg ggccgccttg gggccgcgat
     7441 cgtagcgggc ttcgaaggtc tggacgtcga gcttcagcga gacatcggcc ttgaccacct
     7501 cgccccggcc gatcagccgc gcccgcccgc cctggaaggc ggaggccagc tgttcctcga
     7561 acagaaccga agccggagac acccaacggg ccccggcgat ataggccgcc tcgccacgat
     7621 cactgaccgt caggatccgg tcgccggccg aggcgcgagg aaagccgacg ccgaggcgca
     7681 gcacgccgaa caccggcccc tgggccgtcg gcggagccac ggcgtgggcc tcgaaacggt
     7741 acagctgcac cggctcggcc ttcggaaaca ccgatatgca gcccgacagg gcgatggccg
     7801 ccagcgcaag cgcggcggcg gtcagacgac ggctcacggc tggacctcca cttccctggc
     7861 gggggccttg gagaccacgc cgcgcggatt ctgttcgacc tcgccgacca gccggtcgag
     7921 ggattctgct gtcgcctgca gagacgtgat cgcccgcgtc agctggggca ggccggtatc
     7981 ggcgaagtcg ctggtcgggc cgtcgagctt gccgatcatc gcccgcgcgt cgcgcgtggc
     8041 cgccttcagt tcggtcgccg catcgcccag ctcggccagg gtgcggcggc cgtcgccgtc
     8101 gaccaggccc cgcgtcgtgc gactgagctc ggtgatctcc gtggccgccg catcaatgct
     8161 ctgcagcgcc ttctgggcgt cggcgatgat ttccttgcgc tcgcgcgcct cggccgtcac
     8221 cgcctggacg tcgctcaagg ccgcgctgaa ggtcttcacg ttctcgtccg acaataccct
     8281 gttgacccgg tcgagcgcct cgatcgagcg cgacagcacg gtcccgccgc cttgcagcag
     8341 gtcggacagg gcgctgcgct gggtgcggat caccggcacc tcgccgggtg ggtaggtgtc
     8401 gcgcaacagt cgcttggacg ccgtgccggc ggtgatctgg atgaagttca cgccggtgat
     8461 gccctgaggc tcaagggtgc ccaccgaatc ttcgcgcacc ggcacgtcgg agctcatccg
     8521 ggtgcgggcg ataacgcgcc gcgaatcgcg cgggtcgaga gagatcttgg tcacctcgcc
     8581 gaccttgatg ccgttgaaat gcacctcgcc gccttccgac aggccgttga ccggccccac
     8641 gaacagcacg tcgtagatgt cgtagtcctg gttgaactgc agacgggcca gccagaagac
     8701 gaacaccacc aggcccacga acaggaccag ggaggcgacg ccgaccaggg cgtaattggc
     8761 gtttcgttcc atcagacgtc gtgtcccgtc ctgtgtcgcg acccggcggc ggcccggccc
     8821 cgcgggccca ggaagtattc ctgaatccag ggatgatcgg aatgttcaag ttcgcgcacc
     8881 ggcgcggagg cgacaacctt gcggtccgcc agcaccgcca cgcgggtggt gacagcgtag
     8941 aggctgtcga ggtcgtgagt gatcatgaag accgtgagat tgagactgct ggacaggtcg
     9001 gcgatcaggt cgtcgaaggc cgccgcgccg atcgggtcga gtccggaggt cggttcgtca
     9061 agaaacagca gttgcgggtc gagagccagg gcgcgggcca ggccggcccg cttgatcatg
     9121 ccgccggaca gttccgccgg tttcagggcg ccggcccggg gcggcaggcc ggacagggcg
     9181 atcttgaggt cggccagctc gttgatctcc gcccggctga ggcgggtatg ttcatgcagc
     9241 ggcgcggcga cgttctcgcg cacggtcagc gaagagaaca gcgccccctg ttggaacaac
     9301 accccccagc gccgttcgat cgaggtccag ttcttgcgcg ttgcggtgtc gatatcgtga
     9361 ccgaacagcc gcacgacgcc gccgtccggc tgtttgaggc cgatgatggt gttcatcagc
     9421 accgacttgc cggagccgga cccgccgacg acgccgagaa tctcgccgcg ttggacggtc
     9481 aggtcgaggt catcgtggat gacgtgatcg ccgaactgcg agcgcaggcc gatgatctcg
     9541 atcggcggcg cctccggcgc ttcgaggtcg tcgggcccga tcatatgtcg agctccatgt
     9601 agataaccgc gaaaatggcg tcgatgacga tgatggtgaa gatcgcctgc accacggccg
     9661 aggtcacccg ctggcccagc gagatcacgt cgccgccgac ttccatgccc tggcgcgagc
     9721 cgatgccggc gataacggcg gccatgatcg gggccttgga tatgccgacc cagaagtgcg
     9781 tcgcgccgac gttgtcggcg atccgctgaa ggaagaaggt cggcgacagg tcgaggatat
     9841 tccaggtgat cagcatgccg ccgaccatgc cggatatgtc ggccacgaag gtcagcagcg
     9901 gcatcatcac cagcagcgcg aggaaccggg gcagcacgag ggcgtcgaac ggatcgaccc
     9961 ccatgacctg catggcgtcg atttcctggc gcatcttcat cgccccgatc tcggcggcga
    10021 aggccgaggc cgagcgtccc gcgatcagca ccgcggtgat gacgatgccg aattcgcgca
    10081 gaacggcgat gccgatcgtt tcgacgacga acaccgtggc cccgaagtcg gacagggtgt
    10141 tgatgccgat cagggccacg accgcgccga tgaaggccgt gcccgtcgcc acgatcggca
    10201 gcgcgtcgag gcccgcccgc tcgcactggg cgaaggtcgc cgcccagcgc atccggcgcg
    10261 gattggccag cccccgcaac accgacatgg tcagccggcc aatgaacgcg aaggtttccg
    10321 ccccttcgtg gacgatgttg aagacgccct tgccgatgcg ctcgaacagg tcgaacacac
    10381 catgccgccg ggtcggcggg gtgggctcgg cctccaccgc ggcggcgacc aggtcgagca
    10441 gtcggcgggt ctcgcggcgc gccacgatct gcccggccgt cagctggccc tcggccgcgc
    10501 gcaggatggc gtaggcgccg gcggtgtcgc agcgccgcat gttcatcagg tcgatggtca
    10561 ccgaccgcga tccgtcgagg atgccggcca gccggttgtc ggcgtcgtcg agggattcgg
    10621 cggtccagtc gccggtaagg accacccggg cgttctggtc tccctcctgg agcttgaaat
    10681 cggcgggctt gctcatgaag ggatcgcgcg ggtcctcggg tcgtcgctcg accctgccac
    10741 gcgatgaagt caacgcaaag cggccgtgct cgttgcgtgc aattgtgttc gcctctgtgg
    10801 tgcgcgaggc tccaggggaa acgggggaga gcgcggatga ccacgggcat cgacgggcag
    10861 gccgatttcc gcacgaccgg ctggacgcgc atcgcgaccg gactggcgca gggactggcg
    10921 ctgtgggccc tgaccgagat cgtcgacggc aaggccggga cgaccttcaa tcacgtgatg
    10981 ctgggcgcgc tggaggcggt ggcgctgttc acgcccttca tcgtgctcgg cggactggcc
    11041 gaaatgcggc ggcggaccct ggtgatctgg acggtcgggg cgacggtcgc gctggcgggt
    11101 ctgggggcgc acgacatcgg ccggcgggtc gccgaaacgg cgccgcaatg gctgtcgggc
    11161 gaggcgatgg tgttcttcgc cgccgtattg ttcatcgccc atcatctggt ggccggcgcc
    11221 gaggccgacc gccggccgat cgcgcggttc gcgacctatt tcgacctggc ctggaagcac
    11281 ggcgtgcagc tggccatggc ggccgggttc gtcggcgtgt tctggatcgt cctgttcctc
    11341 ggcggagcgc tgttcaagct gatcggcatc gggttcgtcg aggagctgat ccgcaagagc
    11401 tggttcgcca taccggccac ggcgacgatg ttcgccgccg ccgtgcagct gaccgacgtc
    11461 cggcacgcgc tgatccgcgg ggtccgcacg gtggccctga ccctgctgtc ctggctgctg
    11521 ccggtgctgg tgttcctgac cggggccttc atggcggccc tgccgttcac gggcctggag
    11581 ccgctgtggg cgacgaagag cgccgccgcg atcctgctca gcgccgcggc ggtgatgatc
    11641 atcctgatca acgcggccta ccaggacggc gagcccgacg agccgacgcc cgtggtcctg
    11701 cgctgggcgg cgcgggccgg gggcctgctc ctggtcccgc tggtcgcggt agccgcctac
    11761 gccgtgtggc tgcgcgtcaa ccagtacggc ctgaccccgg accggatcat cgccggcgcc
    11821 tgcgtgatcg caggtgtctg ctacgccgtc ggctacggcg tcgcggcgct gctgcccggc
    11881 gcctggatga agcggctgga ggtcaccaac gtgaccaccg cccttgtcgt ggtcgccctg
    11941 atcctggcgc tgttctcgcc ggtcgccgat ccggcgcggc tgtcggtcaa cgaccagatg
    12001 gcgcggctgg cggcgggcaa ggtcgcgccc gacaaggtgg actaccggtt cctgcgcttc
    12061 gagagcgggc ggtacggcat ggaagcgctg gcgacgttga aggccagggg cggggatatc
    12121 ggcaggcggg cgaccgaggc gctcgcgctg aaggagcggt accggcggga cgtcgccgag
    12181 cagccgcgcc cacgcgacat ggccagccag gtcaccgtct ggccgaggga ccgcaccctg
    12241 ccggacagtt tcatcaaccg gcgctgggat gacgaccccc gttcgccggt ccgctgcgac
    12301 ggcaaggacg acaagtgcga ggccaccttc gccgacgtca acggcgatgg ccgcgaagag
    12361 atcctgctgg ccttctctgg cgccgtgacg gtctatgagg aaggcacgcc cggcgtctgg
    12421 cgcgtggtcg gctggacgga ctccctcaac tgcgacggcg atggcgtggc cgtgggggag
    12481 ggacgggtca aggtcgcgcc caggccctgg gcggacctgc tggtcaacgg tcgccgtgtg
    12541 ccgctgaacg tggagcctgc cggctgtccc gagccggcgt cgcactaggc gccgggtcgc
    12601 atgccatgtc tgttggaccc gggcctgttc cgcgaagccc gttggggcga agcagaatgg
    12661 tggatgcgac agggattgaa cctgtgaccc cctcggtgtg aacgaggtgc tctcccgctg
    12721 agctacgcat ccgctcttgg gctgtccgcc cgggaggcgg ggtctatagc gactcggccg
    12781 cggtccggca agacgccgcg atgtcggcca ccgcccgggc cagggcgtcg aagtggtcga
    12841 tgctcacgtc gccgcccagt tcgtccggcg gcaggtcgtt gtagccgaaa ctgcagacca
    12901 cgcaggggac gccggccgcc ttcgccgcgc cgacatcggg cgaggcgtcg ccgaccagga
    12961 cggcgtggtc gcgatcgccc ccggcgcggg cgatcgccgc gagcaggggg gcaggatccg
    13021 gcttctgcac cggcagggtg tcgccgccga cgatggcgtc gaacaggcgg gtcatgtcga
    13081 gttcgtccag cagcagccgg gccagcccct cgggcttgtt ggtgcagacc gccagggccg
    13141 cgccgctgtc gcgcagggcg gccagggcct cgaggcagcc ggggaacggc cggctctccc
    13201 gggcgatgcg ggcccggtag acgtcgacga tgcgcggccg aagctgcgcg atctgctcct
    13261 cggtgatggt caggtcggcc atggcgaagg cgcggatcag catgccgcgc aggccgtggc
    13321 cgaccagctg ccgcaccgcg gtcatgggcg cgggcgccat gccctgctcg acgaggatca
    13381 cgttcagcgc cccgacgagg tcgtgcgcgg tctccaccag ggtcccgtcg aggtcgaagg
    13441 cgacggtcca gccggcgagg ggcttgctcg ggtcatgcat cgtcgcaggc tttcgggtaa
    13501 agctcgccgg aatgcaacgt cacgagtcga tccgatgagc cagcgcgccg cggtgatcat
    13561 ggccgccggc cagggcaccc gcatgaaatc cccgacgccg aaggtgctgc accgcatcgg
    13621 cggacggacc atgctcgacc gggccatcga cgccgtccag gccgcaggct gcgggcggat
    13681 cgtggtcgtc tgcgggaccc acagcccggc cgtcgccgag catgtgcgca agcgcctggg
    13741 tgagggcgcc gtggccatcc aggacccgcc gctgggcacc gcccatgccg tgctggccgc
    13801 gaaggccgcg ctggcgggct ttgccggcga cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nngcgcatcg tcgaggcgcg cgacgcgacg gccgaggaga
    14041 aggccgtccg cgcctgctat tcgggtatcc tctgcgcccc ggccgggccg ctgttcgact
    14101 ggctggcggc cgtcgacaac aacaacgcta aggccgagta ctacatcacc agcatcgtcg
    14161 gcatcgccaa cgccgccggc aagcgggtcg gcgtggcctt cacctccgag accgccgtca
    14221 tgg
//
LOCUS       DAORNX010000017        13902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3330_length_13902_cov_7.851231, whole genome shotgun sequence.
ACCESSION   DAORNX010000017 DAORNX010000000
VERSION     DAORNX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3330_length_13902_cov_7.851231"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..563
                     /locus_tag="PLO65_01440"
     CDS             48..563
                     /locus_tag="PLO65_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26942.1"
                     /translation="MSFSAARPLDPWRWIGVPTLLCLGATILLAAPIQLFGLRLPEPV
                     FPMVCAFGWAVIRPSMLAPAVLLVMGLFLDIYWGGTLGLWAVSLLLAYAVTLAMRNMM
                     TGQSRPMMWAWYAGMSLVAMATGYLITMLDVKAAPSVVAVVWQFLATAILYPFAHRLI
                     DRFEDADVRFR"
     gene            560..2566
                     /gene="mrdA"
                     /locus_tag="PLO65_01445"
     CDS             560..2566
                     /gene="mrdA"
                     /locus_tag="PLO65_01445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD26943.1"
                     /translation="MSEPTIFFSEVNEKQGAFTRRAFLLGGLTGAGLLALTGRLIHLQ
                     IVEAQRYQKLAQSNQFNFRLVPPPRGLILDRNGVTLASNRPNFRLLVAREPGMDVEKT
                     VADLATLVPIEDRKRERLVRDIKRAPKKAPVQVMEDMSWEEFSRINVRAPELAGVTAD
                     MGEVRVYPYGGAFAHVIGYVARVNDRDIEAARKAGVSPDPLLYNPGFRIGRQGVEKAF
                     DAELRGKAGAQKQEVDAQGRVVRQDPGGDIPATPGKEIRLTLDADIQNRALEVMGEES
                     GAIVLMDCRSGDLLCMVSTPSFDANRFVKGLSGPEYRALAQYERKPLLDKALYGNYAP
                     GSTFKPTVALAALEAGVDPETRVVCTGGWFFGNRTFHCWKKGGHGSQTMHDAIKNSCD
                     VYFYQTALKIGPDIIARTARSMGFGQVFDIGIPGQKRGLVPDRAWKKKAYKDPRNQTW
                     FPGESPSYGIGQGALTVNALQLAVMTSRLANGRKALNPRLIKSVGGVDRPHGDAVPDL
                     PFSQEFLNIVRGGMIAVANDTSGTAYRQSQLGLGDILMAGKTGTAQVRSYDKAASRNS
                     ASVRWGLKDHNLYVAFAPVDAPRYAMSVLIEHGGLGGATAAAPRAREVMRVALLKDPE
                     IRARIEKPLPMPELDEASLAEGAAPGPAVEGPPPPATPAPGAPQ"
     gene            2563..3720
                     /gene="rodA"
                     /locus_tag="PLO65_01450"
     CDS             2563..3720
                     /gene="rodA"
                     /locus_tag="PLO65_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRD26944.1"
                     /translation="MTLSGGLTRPGERDRLIVKFTEIDWTFCLILCLIAGAGGMMMFS
                     AAESSWTPWAAPHLIRFGVFFVMMIVLAMVDLRVWFTAAYPVYALALLLLVAVELFGD
                     SAKGAQRWLELGPLRFQPSEVMKIGIVLALARFYHGLSADHARMSWWLLVPLLLIGAP
                     TLLVAHQPDLGTAILIAATGLAIMVLAGLSWRLIGAGVVLGVGAAIPFVMFVLHDYQR
                     QRILTFLDPESDPRGTGYHILQSKIALGAGGLLGKGYGLGSQSQLDFLPEKHTDFMFA
                     TLAEEFGFAGCFSILFLYGAIIFMALRMASISHSHFGRLSAAGVTATFALYVLINGAM
                     VMGLAPVVGVPMPLLSYGGTVMLTVMVGFGLVQSVRVHRYSEVTSGRGALM"
     gene            3786..4349
                     /locus_tag="PLO65_01455"
     CDS             3786..4349
                     /locus_tag="PLO65_01455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRD26945.1"
                     /translation="MSLVLHTIICSTRPGRVGATIARWFHEAATAHGQFDARLVDIAD
                     FNLPVYDEPRHPVMQSYEHDHTKAWSASVAAADAYVFVTPEYNYGPPPSLVNALNYVY
                     REWNYKPASFVSYGGVSGGLRAVQVEKQILTTLKVMPIPEQVSVHMAGQHIEDGVFKP
                     GPMHEASAGKALDELHRWAGALRVMRG"
     gene            4412..4651
                     /locus_tag="PLO65_01460"
     CDS             4412..4651
                     /locus_tag="PLO65_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRD26946.1"
                     /translation="MASYRDLLVWRKAMDLAEGAYSLAKLMPKGEQFRLTAQLLRAAV
                     SVPANIAEGRARTGKREFLNFLSIASGRWRKPRHC"
     gene            complement(4817..5809)
                     /gene="pip"
                     /locus_tag="PLO65_01465"
     CDS             complement(4817..5809)
                     /gene="pip"
                     /locus_tag="PLO65_01465"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRD26947.1"
                     /translation="MDRSASPPAPGHVTSRRTLYPDVDAYNIGWLPTGGPHELYYEEC
                     GNPRGKAAIVLHGGPGGAINPTMRRFFDPAKWRVALFDQRGCGRSRPNASLEDNTTWS
                     LIEDIERLRVHLGVEKWTVFGGSWGSTLALAYAVTHPERVEGLVLRGVFLLTQRELKW
                     FYQDGASFLFPDAWERFVGPIPKGERHDMIAAYHKRLTHSDRKVQAEAAAAWSQWEGD
                     TISIRGPEARPPKFNEVDFAIAFARIECHFFANRGFFPEDGWLLKQVEKIRKIPGWIV
                     QGRFDVVTPLESAWALHKAWPESRFNIIWDAGHASTEPGIIDALIRATDEAARK"
     gene            complement(5945..6949)
                     /locus_tag="PLO65_01470"
     CDS             complement(5945..6949)
                     /locus_tag="PLO65_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD26948.1"
                     /translation="MSALTAMMPPVQYADVNGIRMAYYEAGPEGEGTPLVFCHGWPEL
                     AFSWRHQIKALSEAGRWVIAPDQRGYGLTDAPAEVESYALENLCADLVALLDHKGIEK
                     AIFVGHDWGGFVVWYMAIRHPDRVAGVIGLNTPFNERAPVDPIAIMRARLGERMYIVH
                     FQTPGEADAVLNGNVRKAMNFFMKRPLDLPAAPSAGFATERKPGDPSAFAIVDMLEAY
                     DPANDPRETLMTDAEFEALVETYERTGFTGGINWYRNFTRNWHASEGLEHRVRQPSLM
                     IMAEKDAVLPPSAADGMERLIPDLEKQLVLGSGHWTQQEEPEQVNRIMLDWLGRRFPQ
                     "
     gene            7217..8440
                     /locus_tag="PLO65_01475"
     CDS             7217..8440
                     /locus_tag="PLO65_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRD26949.1"
                     /translation="MSQSPDIARPSHTPWGLVFLLGSLTAFAPMSIDMYLPAFPAMGA
                     ALGADAGQQQATLAVFLFGMAIGQFLYGPASDRFGRRTPILVGITVYVAASIACALAT
                     GPEWMIAARFVQALGGCAGAVVARAVVRDQFDHTETARMLSLLTLIMGLAPILAPVSG
                     AVVLQALGWRAIFWVLAAFGAICGAAVFLRLKETRSEATAAQARSENPLRAYRVLISN
                     RRLIGYCLGGALNGALLFTYISSSSGLIIETFGFSPFAYTLVFGGNAFGVIGAGQVNR
                     MLLRRLSPDQVLARASLAALGFGVLLLAAAVTGLGAQWTVLPLLFCCLASYGLMAGNT
                     MAGALSVDPTRAGSTSALMGGLSFASGAVASWLGGVLHDGTAVPMAGVMMACLLGSSA
                     ALWGLALPGRRQPAP"
     gene            complement(8425..8781)
                     /locus_tag="PLO65_01480"
     CDS             complement(8425..8781)
                     /locus_tag="PLO65_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRD26950.1"
                     /translation="MTLAAVSGFLAVAVGAFAAHGVADPAAKDWLKTGASYEFMHAMA
                     TFACATFMNIGARRARFAPAFFLGGTALFSGSLYAMALGAPRWLGAVTPLGGLSFLIG
                     WTVLAWSARGVDQGAG"
     gene            8916..10025
                     /locus_tag="PLO65_01485"
     CDS             8916..10025
                     /locus_tag="PLO65_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26951.1"
                     /translation="MALTKGDDFPIHQTAEPIAYSGTDRNFYDRYFFNGYHPDSGEDF
                     FAVAFGVYPHLNIADGAFVVVRGGVETALHVSAVLGDRLDLTAGPLKIEILEPLQKLK
                     VTVDSPEHRLKGEIIFTGRAFPIEEPRFVRRQGTRTFMDYTRLTQNGHYTGWIELDGE
                     RRSVDGYVGTRDRSWGVRPVGARDSQEVVPAQAPQFYWLWSPCSFADGSFFFHSNEDA
                     TGRPWNTRAVWAPDGAGEDGLDHLNGAAAIEWRSNSRHAQSAVVSLTDDYGKPAGQVE
                     FKPLYEFQMMGLGYTHPKWGHGQLHGELAVEREDIVLAEADPTLPFNLHVQALSEVTF
                     TDAAGKQRKGRGVLEQLVIGPHGPSGFTGMMDMAK"
     gene            10022..11035
                     /locus_tag="PLO65_01490"
     CDS             10022..11035
                     /locus_tag="PLO65_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRD26952.1"
                     /translation="MSLQDQLEAYLTRIWQTPARVTMIARIPGGASRETYRFDVEADG
                     ATRALILRRDPVGSLIDTERRLEFLAFRSFHGGGLPVPEGVALEEDGAELERPFFIMG
                     RIDGGKAQSPFTVAPYGEHAAAIGEQFYRHLGTIAAADPATLPIAAAAETPAPDACWK
                     RELDYWEGVIDADEEHPQPIVRACIRRLRASPPPPAQKLSVVHGDYRTGNFLHDGAGK
                     VIALLDWEMAHIGDPLEDLGWAMEPLWCYGETDRVSGMLPRAEAIAIWEAASGLTVDP
                     VAFAWWELFASVKGQAIWISSAKEFRAGGLKDPVLGLSGWYTARRQDIILADRLAALE
                     ALA"
     gene            11032..11463
                     /locus_tag="PLO65_01495"
     CDS             11032..11463
                     /locus_tag="PLO65_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26953.1"
                     /translation="MTPTVPELLLGNFLCLMDPPPPESSGDFLQSRIGVTGMISLLCA
                     QEAERGIAARVWENGAIRDLLTRSAPAYGARFAEAAAGEDSDLSLAALDRANADLRRA
                     LIDLHIAVEAARNAALDTEIRGLYVQMADARRLDLPPLPAG"
     gene            complement(11469..11897)
                     /locus_tag="PLO65_01500"
     CDS             complement(11469..11897)
                     /locus_tag="PLO65_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26954.1"
                     /translation="MRSAVLSLAALAAVVATPALAIDANVIDGVYTGGYRCAQGVTFL
                     ELTVDGDRDGSVRAVFRFNSATWNGGENRTVPEGKFAMAGRLNDQGQIVLQGSYWILQ
                     PRDYAMVNLAGLVTREGGSLVVAGNVGGAPSCTTFRVVRN"
     gene            12033..13190
                     /locus_tag="PLO65_01505"
     CDS             12033..13190
                     /locus_tag="PLO65_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26955.1"
                     /translation="MSRVLRLGLFYCSIFIGTGASLPYMPVWFRAHGLSGAQIGVILA
                     APMLARVVITPFLAVWADGFRLRRTPIMLFGLAAGVCYGLIGLVAGFGPWLLFWLLAA
                     SFLSTVVPLADVLTLRRARAEGFNYGLPRGLGSTAFVVANVGMGALIARTGPEWIVPW
                     VCAAGLLSGLAGRVLLPPDPVHEGGERPDRRDRWRGVSDLLRNRPFLLAVISTSLIQA
                     THAFYYAFSALLWKAQGLSATTVGLLWGTGVAVEIAFMWFLEPWRRRVGPEALVVLGG
                     VAAVVRWSAFAFAPPLWLLFPLQALHALSFAAVFMGSLQLIERHAPARSASAAQTISS
                     AVSGGLVIGLATLASGPLFDALRERGYLAMSALALLGLLGAWRLTLLRLRT"
     gene            13272..13463
                     /locus_tag="PLO65_01510"
     CDS             13272..13463
                     /locus_tag="PLO65_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26956.1"
                     /translation="MQAVIEIVAAMVVWAASVAFAHFGIEIDTTPPPAREEALARRTP
                     ATPAHDPCPDAEKGRIRRV"
     gene            13623..>13902
                     /locus_tag="PLO65_01515"
     CDS             13623..>13902
                     /locus_tag="PLO65_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26957.1"
                     /translation="MTARKPRTPFDFSRVSREVAAQQVAGPVAESELDAPPALEAELD
                     ILQAPPTVEMTAEDTAPPTPVRAGEPVAEPPLPPVVLPRVDRVREPSGS"
BASE COUNT         1962 a         4808 c         4857 g         2275 t
ORIGIN      
        1 gcctcgccgc cgcgtcccgc cacgccggcg ccggccggag gcggacgatg agcttctcgg
       61 ccgcgcggcc gctggacccg tggcgctgga tcggcgttcc cacgctgctg tgcctcgggg
      121 cgaccatcct gctcgccgct cccatccagc tgttcggcct gcgcctgcct gagccggtgt
      181 ttccgatggt ctgcgccttc ggctgggcgg tgatccggcc ctcgatgctg gccccggcgg
      241 tgctgctggt catgggcctg ttcctcgaca tctactgggg cggaaccctc ggcctgtggg
      301 ccgtgtcgct gctgctggcc tatgccgtca ccctggcgat gcgcaacatg atgaccggcc
      361 agagccggcc gatgatgtgg gcctggtacg ccggcatgtc cctggtggcg atggctaccg
      421 gctatctgat caccatgctg gatgtgaagg ccgcgccgag cgtcgtcgcg gtggtctggc
      481 aattcctcgc cacggccatc ctctacccgt tcgcccaccg gctgatcgac cgcttcgagg
      541 atgcggacgt gaggttccga tgagcgaacc gaccatcttt ttcagcgagg tcaacgagaa
      601 gcagggggcc ttcacccgcc gggccttcct gctgggcggc ctgaccggcg ccggcctgct
      661 ggccctgacc gggcggctga tccacctgca gatcgtcgag gcccagcgct accagaagct
      721 ggcccagtcg aaccagttca acttccgcct cgtgccgccg ccgcgcggac tgatccttga
      781 ccgtaacggc gtgaccctgg cctcgaaccg gcccaacttc cgcctgctgg tcgcgcgaga
      841 gcccggcatg gacgtcgaga agacggtcgc cgacctcgcc accctggtgc cgatcgagga
      901 caggaagcgc gagcggctcg tgcgcgacat caagcgggcg cccaagaagg cgcccgtcca
      961 ggtcatggag gacatgagct gggaggaatt ctcccgcatc aacgtccggg ccccggagct
     1021 ggccggggtc accgccgaca tgggcgaggt gcgggtctat ccctacggcg gggctttcgc
     1081 ccacgtcatc ggctatgtcg cgcgggtcaa tgacagggac atcgaggccg cccgcaaggc
     1141 cggggtcagc cccgatcccc tgctctacaa cccgggcttt cgcatcggcc gccagggggt
     1201 cgagaaggcc ttcgacgcgg agctgcgcgg caaggccgga gcccagaagc aggaggtcga
     1261 cgcccagggc cgggtggtgc gccaggatcc cggcggcgac atccccgcca ccccgggcaa
     1321 ggaaatccgc ctgaccctcg acgccgacat ccagaaccgc gcgctcgagg tgatgggcga
     1381 ggagagcggg gccatcgtgc tgatggactg ccgcagcggc gatctgctgt gcatggtctc
     1441 cacgccgagc ttcgacgcca accggttcgt caagggcctg tccggccccg aataccgggc
     1501 cctggcgcag tacgagcgca agccgctgct cgacaaggcg ctctacggca actacgcgcc
     1561 gggctcgacc ttcaagccga ccgtcgccct ggcggcgctc gaggccggcg tcgatccgga
     1621 aacgcgggtg gtctgcaccg gcggctggtt cttcggcaac cgcaccttcc attgctggaa
     1681 gaagggcggg cacgggtcgc agaccatgca cgacgcgatc aagaactcct gcgacgtcta
     1741 tttctatcaa acggcgctga agatcgggcc cgacatcatc gcccgcaccg cccggtcgat
     1801 gggcttcggc caggtgttcg acatcggcat cccaggccag aaacggggct tggtgcccga
     1861 ccgggcgtgg aagaagaagg cctacaagga tccgcgcaac cagacctggt tccccggcga
     1921 gtcgcccagc tacggcatcg gccagggggc cctgacggtc aacgccctgc agctggcggt
     1981 gatgacctcg cgcctggcca acggccgcaa ggcgctgaac ccgcggctga tcaagtcggt
     2041 cggcggcgtc gaccggccgc atggcgacgc ggtgcctgat ctgccgttca gccaggaatt
     2101 cctcaacatc gtgcgtggcg gcatgatcgc ggtggccaac gatacgtcgg gcaccgccta
     2161 tcgccagagc cagctgggtc ttggcgacat cctgatggcc ggcaagaccg gcacggccca
     2221 ggtccgcagc tatgacaagg ccgcctcgcg caacagcgcc agcgtccgct ggggcctgaa
     2281 ggaccacaat ctctacgtcg ccttcgcgcc ggtcgacgcg ccgcgttacg ccatgtcggt
     2341 gctgatcgag catggcggcc tgggcggggc cacggcggcc gcgccgcgcg cccgcgaggt
     2401 aatgcgcgtg gcgctgctca aggaccctga aatccgcgcg cggatcgaaa agccgctgcc
     2461 gatgccggag ctcgatgagg cctcgctggc cgagggcgcg gcgcccggac cggcggtcga
     2521 gggaccgccg ccgcccgcca ccccagcgcc gggagcgccc caatgaccct cagcggcggc
     2581 ctcacccggc ccggcgagcg cgaccggctg atcgtcaagt tcaccgagat cgactggacc
     2641 ttctgcctga tcctctgcct gatcgcgggg gccggcggga tgatgatgtt ttcggccgcc
     2701 gagtcgtcct ggacgccctg ggccgcgccg cacctgatcc gcttcggcgt cttcttcgtg
     2761 atgatgatcg tgctggccat ggtcgacctg agggtctggt tcaccgccgc ctatccggtc
     2821 tacgccctgg ccctgctgct gctggtcgcg gtcgagctgt tcggcgactc ggccaagggc
     2881 gcgcagcgct ggctcgagct cggcccgctg cgcttccagc cctcggaggt gatgaagatc
     2941 ggcatcgtgc tggccctggc gcggttctac cacgggctgt cggccgacca cgcgcgcatg
     3001 agctggtggc tgctcgtgcc gctgctgctg atcggcgcgc cgacgctgct ggtggcgcac
     3061 cagccggacc tgggcaccgc catcctgatc gcggccaccg gcctggccat catggtgctg
     3121 gccggcctct cctggcggct gatcggcgcg ggcgtggtgc tcggcgtcgg ggcggccatt
     3181 ccgttcgtga tgttcgtgct gcacgattac cagcgacagc ggatcctgac cttcctcgat
     3241 cccgagagcg atccgagggg caccggctac cacatcctgc agtccaagat cgccctgggc
     3301 gccggcggcc tgctgggcaa gggctacggc ctgggcagcc agagccagct cgacttcctg
     3361 cccgagaagc acaccgactt catgttcgcc accctggcgg aggagttcgg attcgccggc
     3421 tgcttttcga tcctgttcct ctacggggcg atcatcttca tggccctgcg catggcgtcg
     3481 atcagccaca gccatttcgg ccggttgtcg gccgccggcg tgaccgcgac cttcgccctc
     3541 tatgtgctga tcaacggggc catggtcatg ggtctggccc cggtggtggg cgtgcccatg
     3601 ccgctgctca gctacggcgg cacagtcatg ctgacggtga tggtcggctt cgggctggtg
     3661 cagtcggtgc gcgtgcaccg ctattccgag gtcaccagcg gacgcggcgc cctgatgtag
     3721 cccgtctttg cgggtgacaa ggccggcaat ttggaacagg agtggtggcg aaaaggagtc
     3781 tcgccatgtc cctcgttctt cacaccatca tctgcagcac ccggccgggc cgcgtcggcg
     3841 cgaccatcgc ccgctggttc catgaggccg cgacggcgca cggccagttc gacgcccgcc
     3901 tggtcgacat cgccgacttc aacctgccgg tctatgacga gccgcgacat ccggtgatgc
     3961 agagctacga gcacgatcac accaaggcct ggagcgccag cgtggccgcc gccgacgcct
     4021 atgtgttcgt cacgcccgag tacaactacg gcccgccgcc gtcgctggtg aacgcgttga
     4081 actacgtcta ccgcgaatgg aactacaagc cggccagctt cgtcagctac ggcggggtgt
     4141 ccggtggcct gcgggcggtg caggtggaaa agcagatcct gaccacgctg aaggtcatgc
     4201 cgattccgga gcaggtctcg gtgcacatgg ccggccagca tatcgaggac ggggtgttca
     4261 agcctgggcc gatgcacgag gcgtcggccg gcaaggcgct ggatgaactg caccggtggg
     4321 cgggggcgtt gagggtgatg cggggttgat ttttcgccga tacaactaaa aatggtagga
     4381 ggtagggagt agggagtagg gagtagggct gatggcgtcc tatcgagacc tgcttgtctg
     4441 gcgaaaagcc atggatctgg cggagggcgc ctactcgctg gccaagctta tgccgaaggg
     4501 ggagcagttt cggctcaccg cgcagctact tcgcgccgct gtgtccgtcc cggcaaacat
     4561 cgccgaagga agagcccgaa ccggcaagcg tgagtttctg aattttctca gcatcgccag
     4621 tggtcgctgg cggaaaccga gacactgctg atgcttgctg ttcgaacagg cctgcttcaa
     4681 cggggagatg ttctgcctgt gtttcagacg gccgaggagg tcgggcggat gctgaccgcg
     4741 ttgaagcaaa ccctgaggga gcgggcggcc gccgcctcct gatcgcgcta ctccctactc
     4801 cctactccct actcccttac ttccgcgccg cctcatccgt cgcccggatc agggcgtcga
     4861 tgatgcccgg ctccgttgag gcgtggcccg cgtcccagat gatgttgaag cggctttcgg
     4921 gccaggcctt gtgcagggcc caggcgcttt ccagcggggt caccacgtcg aagcggccct
     4981 gcacgatcca gccggggatc ttgcggatct tctcgacctg cttgagcagc cagccgtcct
     5041 ccggaaagaa gccccggttg gcgaagaagt ggcattcgat gcgggcgaag gcgatcgcga
     5101 agtcgacctc gttgaacttc ggcggtcggg cctccgggcc gcggatcgag atggtgtcgc
     5161 cctcccactg gctccaggcg gcggcggcct cggcctggac cttgcggtcg gagtgggtca
     5221 gccgcttgtg gtaggcggcg atcatgtcgt gccgctcgcc cttggggatc ggcccgacga
     5281 accgctccca ggcgtcgggg aacaggaacg atgcgccgtc ctggtagaac cacttcagct
     5341 cgcgctgggt cagcaggaac acgccgcgca gcaccaggcc ctcgacccgt tcgggatggg
     5401 tgacggcata ggccagggcg agggtcgagc cccaggagcc gccgaacacg gtccatttct
     5461 cgacgcccag atgcacgcga agccgctcga tgtcctcgat cagcgaccag gtggtgttgt
     5521 cctcgaggct ggcgttgggc cggctccggc cgcagccgcg ctggtcgaac agggccacgc
     5581 gccatttcgc cgggtcgaaa aagcgccgca tggtcgggtt gatcgccccg cccggtcctc
     5641 cgtgcagcac gatggccgcc ttccccctgg ggttgccgca ctcctcgtaa tagagctcgt
     5701 gcggcccgcc ggtcggcagc cagccgatgt tgtaggcgtc aacgtccgga tacagggtcc
     5761 ggcgcgacgt gacatgaccg ggggcgggcg gcgacgctga tcgatccatg ccgccgttct
     5821 accgccccgg accgtcgggg gccaatgaca aacggggaag gaggggacat gtaccgtctt
     5881 cggtacgtgt cccccaatcg agttgtgggc ttcggggaca cgtaccgaag gcggtacatg
     5941 tcccctattg tggaaaccgc cggccgagcc agtcgagcat gatgcggttg acctgctcgg
     6001 gctcctcctg ctgggtccag tggcccgagc cgagcaccag ctgcttttcc aggtccggga
     6061 tcagccgctc catgccgtcg gcggcagagg gtggcaggac cgcgtccttc tcggccatga
     6121 tcatcaggct cggctgcctg acccggtgtt caagcccctc ggaggcgtgc cagttgcggg
     6181 tgaagttgcg gtaccagttg atgccgccgg tgaagcccgt gcgctcgtag gtctcgacca
     6241 gggcctcgaa ctcggcgtcg gtcatcaggg tttcgcgcgg atcgttggcc gggtcgtagg
     6301 cctcgagcat gtcgacgatc gcgaaggcgc tgggatcgcc cggcttgcgc tcggtggcga
     6361 agccggcgct cggcgcggcg ggcaggtcca gcggccgctt cataaagaag ttcatcgcct
     6421 tgcggacgtt gccgttgagc accgcgtcag cctcgccggg cgtctggaag tgaacgatgt
     6481 acatccgctc gcccagccgg gcgcgcatga tggcgatcgg gtcgaccggc gcccgctcgt
     6541 tgaacggcgt gttcaggccg atcaccccgg cgacccggtc gggatggcgg atggccatgt
     6601 accagacgac gaaaccgccc cagtcgtgac cgacgaagat cgccttctcg atgcccttgt
     6661 ggtcgagcag ggcgacgagg tcggcgcaga ggttttccag ggcgtagctt tcgacctcgg
     6721 ccggtgcgtc ggtcaggcca tagccgcgct ggtcgggcgc gatcacccag cggccggcct
     6781 cggacagggc cttgatctgg tggcgccagg agaaggcgag ctcgggccag ccgtggcaga
     6841 acaccagcgg cgtgccttcg ccttcgggcc cggcctcgta gtaggccatg cggatgccgt
     6901 tgacgtcggc gtactgcacg ggcggcatca tggcggtgag ggcggacatc ggcgtttccc
     6961 cgggtggtgt ttcactcagc ctgaccgggc gtcgtggggg aaggcaagag cgacttcatt
     7021 cctctccgcg aagcgggcag gggaaccatg cggagcatgg tggagaggca gcgccgggct
     7081 gcccggagag accggaattc aggatagggc cgctcacctc ggcgctgccc cttccccgcg
     7141 cgctgcgcgg tccccctctc cagctatgct gaggaggatt gagttggggc gcatccgtcc
     7201 ctaaacacgc cccatcttga gccagtcccc cgacatcgcc cgtccgtccc acaccccctg
     7261 ggggctggtg ttcctgctcg ggtcgctgac ggccttcgcg ccgatgtcga tcgacatgta
     7321 cctgccggcc ttcccggcga tgggcgcggc cctgggggcg gacgccggcc agcagcaggc
     7381 gaccctggcc gtgttcctgt tcggcatggc catcggccag tttctctacg ggccggcgtc
     7441 ggaccggttc ggacggcgga cgccgatcct ggtcggcatc accgtctatg tcgccgcctc
     7501 gatcgcctgc gccctggcga caggtccgga atggatgatc gccgcccgct tcgtccaggc
     7561 gctgggcggc tgcgccgggg ccgtggtcgc ccgggccgtc gtgcgggacc agttcgacca
     7621 taccgagacg gcccgcatgc tctcgttgct gaccctgatc atgggcctgg ccccgatcct
     7681 ggccccggtg tccggagcgg tggtgctgca ggcgctgggc tggcgcgcga tcttctgggt
     7741 tctggcggcc ttcggcgcga tctgcggcgc ggcggtgttc ctgcgcctga aggagacccg
     7801 gtcggaggcg acggcggccc aggcccggtc ggaaaatccg ctgcgcgcct accgcgtgct
     7861 gatctccaac cggcggctga tcggctactg cctgggcggc gcgctgaacg gggccctgct
     7921 gttcacctac atctcctcct cctccggcct gatcatcgag acgttcggct tctcgccgtt
     7981 cgcctacacc ctggtgttcg gcggcaacgc cttcggggtg atcggcgcgg gccaggtcaa
     8041 ccgcatgctg ctgaggcggc tgtcgcccga ccaggtgctg gcccgggcca gcctggcggc
     8101 cctggggttc ggcgtcctgc tgctggcggc cgcggtgacc ggcctcggcg cgcaatggac
     8161 cgtgctgccg ctgctgttct gctgcctcgc cagctacggc ctgatggccg gcaacaccat
     8221 ggccggcgcc ctcagcgtcg acccgacccg ggccggctcg acctcggccc tgatgggcgg
     8281 gctgtcgttc gcctcgggcg ccgtcgcctc ctggctgggc ggggtgctgc atgacggcac
     8341 cgccgtgccg atggccgggg tgatgatggc ctgcctgctg ggctcgagcg cggccctgtg
     8401 ggggctggcg ttgcccgggc ggcgtcagcc ggcgccctga tccacgcctc gcgccgacca
     8461 ggccaggacc gtccagccta tgaggaagct cagcccgccg agcggcgtca ccgcgcccag
     8521 ccagcgcggc gcgcccagcg ccatggcgta gagcgagccg gagaacagcg ccgttccgcc
     8581 gaggaagaac gccggcgcga aacgcgctcg tctggccccg atgttcatga aggtggcgca
     8641 ggcgaaggtc gccatggcgt gcatgaattc gtagctggcg cccgtcttca gccagtcctt
     8701 cgcggccggg tcggccacgc cgtgcgcggc gaaggccccg acggcgacgg cgagaaaccc
     8761 gctcaccgcc gcgagggtca tccaggtccg ccgatcccag gtcacattga cgctccgtca
     8821 ccgttgcatt cgccccgcgc gaccttacgc tgagccgatc cagcatcaaa cgcccgtttg
     8881 aagaggcgga gctctgttca gggagaacgg cgaccatggc gctcaccaag ggggacgatt
     8941 tccccatcca ccagacggcc gagccgatcg cctattcggg caccgaccgg aacttctacg
     9001 accgctattt cttcaacggc tatcacccgg acagcggcga ggacttcttc gccgtggcgt
     9061 tcggcgtcta tccgcacctg aacatcgccg acggggcctt cgtggtcgtg cggggcggcg
     9121 tcgagacggc cctgcacgtc agcgcggtgc tgggcgatcg cctggacctg acggccggcc
     9181 cgctgaagat cgagattctc gaaccgctgc agaagctcaa ggtcaccgtc gattcccccg
     9241 aacaccggct gaagggcgag atcatcttca ccggccgcgc gttcccgatc gaggagccgc
     9301 gtttcgtccg ccgccagggc acgcggacct tcatggacta cacgaggctg acccagaacg
     9361 gccactacac cggctggatc gagctcgacg gcgagcggcg ttcggttgac ggctatgtcg
     9421 gcacccgcga ccggtcctgg ggcgtgcgcc cggtcggcgc ccgtgacagc caggaggtcg
     9481 ttccggccca ggcgccgcag ttctactggc tctggtcgcc gtgcagcttc gccgacggca
     9541 gcttcttctt ccacagcaac gaggacgcca cgggccggcc gtggaatacc cgcgccgtct
     9601 gggcgcccga cggggccggc gaggacgggc tggatcatct caacggggcg gcggccatcg
     9661 aatggcgcag caactcgcgc catgcgcagt cggccgtggt cagcctgacc gacgactacg
     9721 gcaagccggc gggccaggtg gagttcaagc cgctctacga gttccagatg atgggcctgg
     9781 gctacaccca tcccaaatgg ggccatggcc agctgcacgg ggagctggcc gtcgagcgcg
     9841 aggacatcgt gctggccgag gccgatccga ccctgccgtt caatctgcac gtccaggccc
     9901 tgtcggaggt caccttcacc gacgcggccg gcaagcagcg caagggccgc ggcgtgctcg
     9961 agcagctggt catcggcccg cacggcccgt ccggcttcac cggcatgatg gacatggcca
    10021 aatgagcctg caggatcagc ttgaggccta cctcacgcgg atctggcaga cgccggcgcg
    10081 ggtcacgatg atcgcgcgga ttccgggcgg cgccagccgc gagacctatc gcttcgatgt
    10141 cgaggcggac ggcgcgacgc gggccctgat cctgcgccgc gacccggtcg gcagcctgat
    10201 cgacaccgag cgcaggctgg agttcctcgc cttccgttcg ttccatggcg gcggcctgcc
    10261 ggtgcccgag ggcgtggcgc tggaggagga cggggcggag ctggagcggc cgttcttcat
    10321 catgggccgc atcgatggcg gcaaggcgca gagcccgttc acggtcgcgc cctacggcga
    10381 gcatgcggcg gccatcggcg agcagttcta ccgccatctg ggcacgatcg ccgccgccga
    10441 tccggcgacc ctgccgatcg ccgccgccgc cgagacgccc gcgcccgacg cctgctggaa
    10501 acgggaactc gactactggg agggcgtcat cgacgccgac gaggaacatc cccagccgat
    10561 cgtccgggcc tgcatccgcc gcctgcgcgc cagtccgccg ccgccggcgc agaagctgtc
    10621 ggtggtccat ggcgactacc gcacgggcaa cttcctgcac gacggcgcgg gcaaggtgat
    10681 cgccctgctg gactgggaga tggcccatat cggcgatccg ctggaggacc tcggctgggc
    10741 catggagccg ctgtggtgct acggcgagac cgaccgcgtc tcgggcatgc tgccgcgcgc
    10801 ggaggccatc gccatctggg aggcggccag cggcctgacc gtcgatcccg tggccttcgc
    10861 ctggtgggag ctgttcgcct cggtcaaggg ccaggcgatc tggatttcct cggcgaagga
    10921 gtttcgcgcc ggcggcctga aggatccggt gctcggcctg tcgggctggt acacggcgcg
    10981 ccggcaggac atcatcctgg ccgaccggct cgcggccctg gaggcgctgg catgaccccg
    11041 accgttcccg aactgctgct gggcaacttc ctctgcctga tggacccgcc gccgcccgag
    11101 tcgagcggcg acttcctgca gagccgcatc ggcgtgaccg gcatgatctc gctgctgtgc
    11161 gcccaggaag ccgagcgcgg catcgccgcc cgggtgtggg agaacggcgc catccgcgat
    11221 ctgctgaccc ggtcggcccc ggcctatggc gcgcgcttcg ccgaggccgc cgccggcgag
    11281 gatagcgacc tgtccctggc cgccctcgac cgggccaatg ccgacctgcg ccgggcgctg
    11341 atcgacctgc acatcgccgt cgaggccgcc cgcaacgcgg cgctcgatac cgagatccgc
    11401 ggcctgtacg tgcagatggc cgacgcccgg cggctggacc tgccgccgct gccggcgggg
    11461 taggggctct agtttcggac gacccggaac gtggtgcagc tcggcgcccc gccgacattc
    11521 ccggccacga ccagcgaccc tccctcgcgg gtcacgagcc cggccagatt gaccatggcg
    11581 tagtcgcgcg gttgcaggat ccagtagctg ccctgcagca cgatctggcc ctggtcgttg
    11641 agacggccgg ccatcgcgaa cttgccttcc ggcaccgttc ggttctcgcc gccgttccag
    11701 gtggcgctgt tgaaccggaa tacggcccgg accgacccgt cgcggtcgcc gtccacggtg
    11761 agctcgagga atgtcacgcc ctgggcgcag cggtatccgc cggtgtagac gccgtcgatg
    11821 acgttcgcat cgatcgccag ggccggggtc gcgacaacag cggccagggc cgccagggac
    11881 aggactgctg aacgcatgga cgagcctccc gaagatcgtc ggcaggctgc cgtggccggc
    11941 ggggggcggt cttgccgaaa aaggggggcg ccgatccggg tcggtgatca ggcggggttc
    12001 gccagtcgcg cgcgactctg ctagcgcagg ccatgtcccg tgtgctccgg ctcggcctgt
    12061 tctactgctc gatcttcatc ggcaccgggg ccagcctgcc ctacatgccg gtgtggttcc
    12121 gcgcccacgg gctcagcggg gcccagatcg gcgtgatcct cgccgcgccg atgctggcgc
    12181 gggtggtcat cacccccttc ctggcggtct gggccgacgg ctttcggctg cgccgcacgc
    12241 cgatcatgct gttcggcctg gcggccggcg tctgctacgg gctgatcggc ctggtcgccg
    12301 gtttcgggcc ctggctgctg ttctggctgc tggccgccag cttcctgtcg accgtggtcc
    12361 cgctcgccga cgttctcacc ctgcgccggg ccagggccga ggggttcaac tacggcctgc
    12421 cgcggggcct ggggtcgacg gccttcgtgg tggccaatgt cggcatgggc gccctgatcg
    12481 cgcggaccgg acccgagtgg atcgtcccct gggtctgcgc ggcggggctg ctgagcggcc
    12541 tggccggacg cgtcctgttg ccgcccgatc cggtgcatga ggggggcgag cggcccgacc
    12601 ggcgcgaccg ctggcgcggg gtctccgacc tgctgcgcaa ccggccgttc ctgctggctg
    12661 tgatctcgac cagcctgatc caggccaccc atgccttcta ctacgccttc tcggcgctgc
    12721 tgtggaaggc gcagggcctg tcggcgacga ccgtgggcct gctgtggggc acgggggtgg
    12781 ccgtcgagat cgccttcatg tggttccttg agccgtggcg ccggcgggtc gggcccgagg
    12841 cgctggtggt cctgggcggc gtcgcggcgg tggtccgctg gagcgccttc gccttcgccc
    12901 cgccgctgtg gctgttgttc ccgctgcagg ccctgcacgc cctgagtttc gcggcggtgt
    12961 tcatgggctc gctgcagctt atcgagcgcc atgcgccggc gcgcagcgcc tcggcggccc
    13021 agacgatcag ctcggccgtc tccggcggcc tggtcatcgg cctggcgacc ctcgccagcg
    13081 gtccgctgtt cgacgccctg cgcgagcgtg gctacctggc catgagcgcc ctggcgctgc
    13141 tcggcctgct cggcgcctgg cggctgacgc tgttgcgcct ccgaacatga caaattcgtc
    13201 atgcccggcc cggttgcgaa gcggcccggg caggcggata acgacggtat cgcaggtcgg
    13261 gggacagccc ggtgcaggca gtgatcgaga tcgtggcggc gatggtggtc tgggccgcct
    13321 cggtcgcctt cgcccatttc gggatcgaga tcgacaccac gcccccgccg gcgcgggagg
    13381 aggccctggc ccggcgcacg cccgcgacgc ctgcgcacga tccctgtccg gatgccgaaa
    13441 agggccgcat ccggcgcgtt tgacgccttc aggcggttcg tcatcgcccg gacgttaacg
    13501 aaaccttttc aagccctttt cccgcgcgag tttcaccggt aggtttcacc cagcgcgcga
    13561 ggtgaggggc tggcgatcgt gcgcggcgct ctcctcggcc ccagcaggac cacgacccgc
    13621 gaatgacggc caggaagccc cgtaccccgt tcgacttctc cagggtctcg cgggaagtcg
    13681 ccgcgcaaca ggtcgccggg ccggtcgccg agagcgagct cgatgcgccg cccgcgctcg
    13741 aggccgagct ggacattctg caggcgccgc cgacggtcga gatgaccgcc gaggacaccg
    13801 ccccgccaac gccggtccgg gcgggtgagc ccgtcgccga accgcccctg ccgccggtcg
    13861 tcctgccgcg ggtcgaccgg gtccgcgagc cgtcgggcag cc
//
LOCUS       DAORNX010000018        13601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3419_length_13601_cov_6.888897, whole genome shotgun sequence.
ACCESSION   DAORNX010000018 DAORNX010000000
VERSION     DAORNX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3419_length_13601_cov_6.888897"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLO65_01520"
     CDS             complement(<1..290)
                     /locus_tag="PLO65_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRD26958.1"
                     /translation="MIRDIPPPPGFGDVLPLAAAPEVLAFLARRRSASALSLQEPGPD
                     PAALEALLTLAARVPDHGKLAPWRFIVLRGEAKATLADRFEAVAAARPDAPRL"
     gene            complement(300..890)
                     /locus_tag="PLO65_01525"
     CDS             complement(300..890)
                     /locus_tag="PLO65_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26959.1"
                     /translation="MRLWILISLFAALVLPDVAAARAADTPLTREALLTLLKTDQVWC
                     SGWRDSDQSCEDIAFIDMLPGDKVRQVSRYRMSAEPNLEMVVRETVSLEGGELCSTFR
                     FNDLEIVVLMDNQPAPIEQAAPILAILAQSMAHLEGKKSCEAYVRDEATGDLKAHVTL
                     DGESAPEFDSIYRLIRPDTRILLRPMFENDEQSTTV"
     gene            1060..1890
                     /gene="cysE"
                     /locus_tag="PLO65_01530"
     CDS             1060..1890
                     /gene="cysE"
                     /locus_tag="PLO65_01530"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRD26960.1"
                     /translation="MARQFEVVEASVAPPVWAALRNEAERAVRDEPTLGSLLNAVVLS
                     HGSMAEALSYQFARKIGDQELRAMSAREIAEEAYEADPTLIAAAEADLKAVFERDPAC
                     KGYVQPFLFFKGFQALQTHRVAHWLWTHSRDTLAFYLQSKMSEVFQVDIHPAAKIGKG
                     IFFDHGTGIVIGETATVGDDVSMLHGVTLGGTGAERGDRHPKIGKGVLLGAGAKVLGN
                     IHVGDYAKVASGSVVLKDVPAHCTAAGVPARLVNCPTCEQPARTMDHTLADEIFDYSI
                     "
     gene            2004..2207
                     /locus_tag="PLO65_01535"
     CDS             2004..2207
                     /locus_tag="PLO65_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HRD26961.1"
                     /translation="MDDNHIKQIEAHLKRTFGNPHFSVKARKQKDSAEVELKGEFIGV
                     IYEDEDEAGSFMFEMAILAEDLE"
     gene            2217..2786
                     /locus_tag="PLO65_01540"
     CDS             2217..2786
                     /locus_tag="PLO65_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HRD26962.1"
                     /translation="MAVPDPRVGRKRADPAWLYARLLGGTWTVLPEPIRRLHALEARL
                     TAVGRARVERGDGLLARWAAAMAGFPPRAEDIEIRVDLERRGAIEIWRRDFGGHRLRS
                     TQEAGRGRRLVERFGPAAFAMALVWDGRRLNLRLRSWRLLGLPMPAWSAPAVTAWESV
                     EDGRFHFFVEIGHPWAGLIVRYQGWLAPV"
     gene            2869..3189
                     /locus_tag="PLO65_01545"
     CDS             2869..3189
                     /locus_tag="PLO65_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26963.1"
                     /translation="MPKSVIKQAVRDGDEFLDDALHALKKAAKHVGEDSQDAMGKAVT
                     DLTKATEKLVEEARTKAVPAVKDAAKDVARAAKDHPVAATALVAAAVALVGLAISRRG
                     GGEA"
     gene            complement(3237..4004)
                     /locus_tag="PLO65_01550"
     CDS             complement(3237..4004)
                     /locus_tag="PLO65_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD26964.1"
                     /translation="MTKRALKRTVREGAFDQAQFHNDDGGKVRRLPVGNGWSPLGRDE
                     DREQGYLKTIKPKSEGQAELIKAIDARNLVMALGPAGTGKTYLAIAKAVEALEAGKVA
                     RIVLSRPAVEAGESIGYLPGDMEDKLAPYLRPLYDALSDRLSMKRVRALIAEGAIEIA
                     PVGYMRGRTLNNAFVVIDEAQNCTYGQLKMLLTRLGWHSTMVVTGDPNQSDLLTGLSG
                     LGDVADRFEVLPNVAVVRLAEQDIVRHPLVAEMLGVL"
     gene            4314..4844
                     /locus_tag="PLO65_01555"
     CDS             4314..4844
                     /locus_tag="PLO65_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRD26965.1"
                     /translation="MTVYALGASAPRLPSDDEYWIAPTASVMGDVILKKNASIWWGAV
                     ARGDTDTLTIGENSNVQDGSVLHADHGIPLVIGANVTVGHMVMLHGCTIGDNSLIGIG
                     SIVLNGAKIGKNCLIGAGSLIGEGKEIPDNSLVMGAPGKVIREVGEQQAMILKASALH
                     YVENWKRFRRELAELG"
     gene            5044..5823
                     /locus_tag="PLO65_01560"
     CDS             5044..5823
                     /locus_tag="PLO65_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD26966.1"
                     /translation="MAYEFITVDREGPVTIFTLNRPEVMNALHSPMHFELDEAFNAFA
                     ADPEQWVGIVTGAGERAFSAGNDLKHQAGGGGMGTPPSGFAGLTSRFDLDKPLIAAVN
                     GVAMGGGFEIALACDIILAAETATFALPEPKVGLAALAGGLHRLPRQIGLKRAMGMIL
                     TARRVPAAEGQALGFVNAVVPAADLMAEARKWAQMICECSPMSIRASKQAVMQGLDQT
                     LPEAIGGQFKFPAVGALFRSEDFKEGPMAFAQKRAPQWKGK"
     gene            complement(5989..7401)
                     /locus_tag="PLO65_01565"
     CDS             complement(5989..7401)
                     /locus_tag="PLO65_01565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD26967.1"
                     /translation="MTNFASLGLAKPLLSALAAEGYTTPTPIQSQAIPLVMDGKDVLG
                     IAQTGTGKTAAFALPILHRLAENRRPAPRRGCRCLVLSPTRELATQIGESFKAYGANL
                     GLSVAVVFGGVKYGPQVRALAGGVDVLVATPGRLIDHINEKVANLSEVEVFVLDEADQ
                     MLDMGFVVPIRRIAKTLPRVRQNLFFSATMPSEIEKLAAELLNPNPSKVSVTPQATTV
                     EKIDQKVLFVEAARKRVLLAELFDEAAMKRVLVFTRTKRGADRVAKGLNAIGVEAASI
                     HGDKSQGQRERALAEFKAGKMRALVATDIAARGIDVDGVSHVIQFELPNVPEAYVHRI
                     GRTARAGANGSAIALVADDERNLLRDIQKVTRQTIPAWDRRNDKGLAQAQAAMASVEP
                     EKPYRDPRAVKAAQQKRKPQALDASGKAQPDRGQRARHRPAGYEPLATGAGGDAPPRR
                     KKPRNRNRNRSGGGAAASAS"
     gene            complement(7640..8224)
                     /locus_tag="PLO65_01570"
     CDS             complement(7640..8224)
                     /locus_tag="PLO65_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HRD26968.1"
                     /translation="MTERAPVTAENREIARVVAETFGGQPEVFTWWDDGRTTSVDILS
                     CVDSPGEGLTAYATLNLSNHPLREVENDLRVELLGACRSATALFPSLLSTCAFNVIEH
                     GAVIGPGVVFPEVTALYDPALAMRHILFTPPFYWNDRPQTLTMPGRTVAWLLALPISD
                     TEFEHVKTKGFRSLLDVMEASRPDVYDLDRPSVV"
     gene            complement(8286..9215)
                     /locus_tag="PLO65_01575"
     CDS             complement(8286..9215)
                     /locus_tag="PLO65_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD26969.1"
                     /translation="MTFGATSTTDDVLSGVALGGKRILVTGVSAGLGVETARALAAHG
                     AQVIGTARDLAKARTATAGIPGIELVAMDLASLASVRACADALVAAGRPFDVVICNAG
                     VMACPFGRTVDGFETQFGTNHLGHFVFVNRIASLLQSGGRVICLSSAGHRFSDVDLDD
                     PNFERTPYTEFGAYGRSKTANALFALAFDQRNRGRGVRAASVHPGGIQTELGRHMTPE
                     LAAGMMKSITEANARTGAPAFEFKTIPQGAATTVWAAVVADADEIGGQYCEDCHVAEP
                     QEEGRGGARGYIRDAARAEALWSKSEEMVGERF"
     gene            complement(9240..9551)
                     /locus_tag="PLO65_01580"
     CDS             complement(9240..9551)
                     /locus_tag="PLO65_01580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRD26970.1"
                     /translation="MGDETEKYGLFGKLVAAPGKRDELIGYLLAGTGAMPGCLLYVVA
                     RDPTDADAVWINEIWASRQHHADSLKLPEVQAAIARARPIIAGFGPERIETAPVGGAG
                     L"
     gene            9833..10411
                     /locus_tag="PLO65_01585"
     CDS             9833..10411
                     /locus_tag="PLO65_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26971.1"
                     /translation="MFRTIVSVASVVATVSVAASAMAGPITAKGFTPVHRLPEGAAPV
                     AVWTCDVDPSIVGVTLTKGARRGEVRISYEIANTGGSAWRSGANQQGVHLVAVNGNTG
                     GAYASDRPLTGAAAPGATMQRFTTPLIRNAFDDFEFGGHVALSISYDPDIFIDGNRCN
                     DDSNAGNNTFRIETGEILAFMNGAATSRTFRP"
     gene            10532..12187
                     /locus_tag="PLO65_01590"
     CDS             10532..12187
                     /locus_tag="PLO65_01590"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HRD26972.1"
                     /translation="MGDRYDYIIIGAGSAGCVLANRLTEGGDRTVLLLEAGGKDNSIL
                     VKMPAGVGSLIKDKGTYNWGFWTEAEPALNDRKLWWPRGKGWGGSSSINGMVYIRGHA
                     RDYDQWRQMGLTGWGYADVLPYFKRSESLEGGGDAWHGDKGPLHVSKAASPNPIYSSV
                     IEAGRQAGYPVTKDFNGYQQEGFGPYQFTIKDGERWSSAKAYLYPALSRKNLTCVTGA
                     RTSRIVIEGGRATGVEYLVDGASVTAHANAEVLLCAGAVQSPQILQLSGIGDPEDLTP
                     HGIKVVHALPGVGKNLQDHLDVTLSWLTRGVQTAYSYNKGFKQLLTGLNYLLFKKGFG
                     RQNFLESGAFLRSRPDLDRPDLQIHTVLAIMQNHGKTVIEKDGFTLHVCQLRPESRGR
                     VGLRSADPNDDPTIFANYLATEEDRRAIREGVKMARQVAAQSALRPYIEEEFEPGPAV
                     RTDDEIDAWIRAKAETIYHPVGTCRMGVAGDAQAVVDAELNVIGIQGLRVVDASVMPT
                     LIGGNTNAPTMMIAEKISDVILGKPALPPQDAPVHDGEAVKAA"
     gene            12370..>13249
                     /locus_tag="PLO65_01595"
     CDS             12370..>13249
                     /locus_tag="PLO65_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD26973.1"
                     /translation="MEHRPLGRSGLAIAPLMLGGNVFGWTADEATSHAILDAFVAGGF
                     NAIDTADVYSAWVPGNTGGDSEKVIGTWLSRRRRRDDVVIATKVGMWPVQPGLKAANI
                     VAACEGSLQRLQTDHIDLYQSHRDDADTPQDETLEAYDRLIKAGKVRAIGASNFETAR
                     LDSALDIARAKGLPRYDTLQPAYNLMDRSVEGPLQQLCLDEDVSIISYYGLASGFLTG
                     KYRSSADLGKSPRGGGVKKHLESERGPKVLAALDAVSQALGATPAQVALAWVMARPAV
                     AAPIASATSLEQLEELM"
     assembly_gap    13250..13395
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13396..13588)
                     /locus_tag="PLO65_01600"
     CDS             complement(<13396..13588)
                     /locus_tag="PLO65_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26974.1"
                     /translation="MVKADILADGVAPVGMEVLLAPLRGPRGDVDRFIGLYQPTAMIA
                     RLLGRPALALALGGILRGES"
BASE COUNT         2160 a         4543 c         4683 g         2069 t
ORIGIN      
        1 agcctgggcg cgtcggggcg ggcggcggcg accgcttcga accggtcggc cagagtcgcc
       61 ttggcctcgc cgcgcaacac gatgaagcgc cagggggcga gcttgccgtg atcgggaacc
      121 ctggcggcca gggtcagcag cgcctccagc gccgcggggt ccgggccggg ttcctgcagc
      181 gacagggccg aggccgaccg ccgccgggcg agaaacgcca gcacctcggg cgcggcggcc
      241 agcggcagca cgtcgccgaa gccgggcggg ggcgggatgt ctcggatcat gggccttgcc
      301 tagaccgtgg tcgactgctc gtcgttctcg aacatcggac gcagcaggat gcgggtgtcc
      361 ggccggatca gccggtagat ggagtcgaac tccggcgcgg attcgccgtc gagggtgaca
      421 tgggccttca gatccccggt ggcctcgtcg cgcacatagg cctcgcacga cttcttgccc
      481 tccaggtggg ccatcgactg ggccaggatg gcgaggatcg gcgcggcctg ctcgatcggc
      541 gcgggctggt tgtccatcag cacgacgatc tcgagatcat tgaaccggaa ggtcgagcac
      601 agctcgccgc cctcgaggct gaccgtctcg cgcacgacca tctcgaggtt gggctcggcc
      661 gacatgcgat accggctgac ctggcgcacc ttgtcgccag gcagcatgtc gatgaaggcg
      721 atgtcctcgc agctctggtc ggagtcccgc catcccgagc accagacctg gtcggtcttg
      781 agcagggtta gcaaggcctc gcgggtcagg ggcgtgtcgg ccgcccgggc ggcggcgaca
      841 tccggcagga cgagggcggc gaacagggaa atcaggatcc agaggcgcat ggtgtcaggc
      901 tagtgccttt cgcgggtgaa tggaaaccgc gacggcctgt ccggccgcgg tccttggcgc
      961 tcgcccctct gctccccata tgcggcgaaa gggcggtcgt gtcggcgaat tagcagggat
     1021 tgtgctatag acggcatgag ggattacggg aggcccggca tggccaggca gtttgaagtc
     1081 gtcgaggcgt ccgtggcgcc gcccgtctgg gccgccctgc gcaacgaggc ggagcgggcc
     1141 gtgcgcgacg agccgacgct gggcagcctg ctcaacgcgg tggtgctgtc gcatggctcg
     1201 atggccgagg ccctgtccta ccagttcgcg cgcaagatcg gcgaccagga gctgcgggcc
     1261 atgagcgcgc gcgagatcgc cgaggaggcc tatgaggccg acccgacgct gatcgccgcc
     1321 gccgaggccg acctcaaggc ggtgttcgag cgcgacccgg cctgcaaggg ctatgtccag
     1381 ccgttcctgt tcttcaaggg cttccaggcg ctgcagacgc accgggtggc gcactggctg
     1441 tggacccaca gtcgcgacac tctggccttc tatctgcaga gcaagatgag cgaggtcttc
     1501 caggtcgaca tccatccggc cgcgaagatc ggcaagggca tcttcttcga ccatggcacg
     1561 ggcattgtca tcggcgagac cgccacggtc ggcgacgatg tctccatgct gcacggcgtg
     1621 acgctcggcg gcacgggggc cgagcgcggc gaccgccacc ccaagatcgg caagggcgtt
     1681 ctgctcggcg ccggcgccaa ggttctgggc aacatccacg tcggcgacta cgccaaggtg
     1741 gcctccggct cggtggtgct gaaggacgtg ccggcccact gcacggccgc cggcgtgccg
     1801 gcccggctgg tcaactgccc gacctgcgag cagccggccc ggaccatgga ccacaccctg
     1861 gccgacgaga tcttcgacta cagcatctga ccgaaggggt cgggcgcggc gaattttccg
     1921 gtccctccgg cgcaaatccc gtttcacctt tccgccgcgg ggctctaggt tccgcggcca
     1981 atccccatgc ccggagcctc cccgtggacg acaatcatat caagcagatc gaagcccacc
     2041 tgaagcgcac cttcggcaac ccgcacttct cggtgaaggc ccgaaagcag aaggattcgg
     2101 ccgaggtcga gctgaagggc gagttcatcg gcgtgatcta cgaggacgag gacgaggccg
     2161 gctcgttcat gttcgagatg gccatcctcg ccgaagacct ggagtagccg ccggccatgg
     2221 ccgttcccga cccgcgggtc ggtcgcaagc gtgctgatcc ggcctggctg tacgcccgcc
     2281 tgctcggcgg aacctggacg gtccttcccg aaccgatccg gcgcctgcat gcgctggagg
     2341 ctcgcctgac cgccgtcggc cgcgccaggg tcgagcgggg cgacggcctg ctcgcgcgat
     2401 gggccgcggc catggccggc tttccgcccc gtgcggagga catcgagata cgcgtcgacc
     2461 tggagcggcg cggcgcaatc gagatatggc ggcgcgattt cggcggtcac cgactgcgga
     2521 gcacccagga ggccggccgt gggcgacgcc tggtcgagcg gttcgggccc gcggctttcg
     2581 ccatggccct ggtctgggac ggccggcgac tgaacctgcg tctgcggtcc tggcggctgc
     2641 tgggcctgcc gatgccggcc tggtcggcgc ccgcggtcac ggcctgggaa agtgtcgagg
     2701 acggccgttt ccacttcttc gtcgagatcg gccacccgtg ggccgggctg atcgtgcgct
     2761 atcagggctg gctggcgccg gtttgacatc cgtcaacgcg gcggggccga tgcggaacca
     2821 aggtgcctcg cacgccgttg caggcgtcct ctcccaggga gacccaagat gcccaaatcc
     2881 gtgatcaaac aggccgtccg cgacggcgac gagttcctcg acgacgccct gcatgccttg
     2941 aagaaggccg ccaaacacgt tggcgaagac agccaggacg ccatgggcaa ggccgtgacc
     3001 gacctgacca aggcgaccga gaagctggtc gaggaggccc gcaccaaggc cgttccggcg
     3061 gtcaaggacg cggccaagga cgtggccagg gcggccaagg accacccggt ggcggcgacc
     3121 gccctggtgg ccgccgccgt ggccctcgtc gggctggcga tcagccggcg cggcggcggc
     3181 gaagcctgaa acccgcgggc ggggccgttg aggcggcctc gcccacgggt tcccgttcag
     3241 agcacgccca gcatttccgc gaccagcgga tgacgcacga tgtcctgttc ggcgagccgc
     3301 acgacggcga cgtttggcag gacctcgaag cggtccgcga cgtcgccgag gccggacagg
     3361 ccggtcagca ggtccgactg gttgggatcg cccgtcacca ccatggtcga atgccagccc
     3421 agacgggtca gcagcatctt gagctggccg taggtgcagt tctgcgcctc gtcgatgacc
     3481 acgaaggcgt tgttcagcgt gcggccgcgc atgtagccca ccggcgcgat ctcgatggcg
     3541 ccctcggcga tcagcgcccg gacccgcttc atgctgaggc ggtccgacag ggcgtcgtag
     3601 agcggccgca ggtagggggc cagcttgtct tccatgtcgc cgggcagata gccgatcgat
     3661 tcaccggcct cgaccgccgg tcgcgacagc acgatcctcg ccaccttgcc cgcctcgagc
     3721 gcctccacgg ccttggcgat cgccagatag gtcttgccgg tgcccgccgg gcccagggcc
     3781 atgaccagat tcctggcgtc gatcgccttg atcagctcgg cctggccctc ggattttggt
     3841 tttattgtct tgagataccc ctgctcgcga tcctcatccc ggccgagcgg cgaccagcca
     3901 tttcccacgg gcaggcgacg gaccttgccg ccgtcatcgt tgtgaaactg ggcctggtcg
     3961 aatgcgcctt cgcgcacggt ccgcttcagg gcacgcttgg tcatggggta gcctccggtg
     4021 gggcatgaaa aaagggcgag gccgcgaagg ctcgcccttg gttcgaggtg acggacggga
     4081 tgacgcgtgc gcggcccggc ggctcgctgc agcttcgcgg aactggcggc gcccaatgac
     4141 gccggaacgc aatacgcggc acccgtttct ccgtcgtcag gagaccgtgg acgaatgtcc
     4201 acgggcggga tgcgaagtag ctgtcgcccc ttcgaatcgc ctgactatga tgatgctaac
     4261 gtggtttcgg actcgttaat caagcggcta tttcaaatat acggggcgta cggatgactg
     4321 tttatgcttt gggcgcttcg gccccgcggc tgccttcgga cgatgaatac tggatagcgc
     4381 ccacggcatc cgtgatgggc gatgtgattc tgaagaaaaa cgccagcatc tggtggggcg
     4441 cggtcgctag aggcgacacc gatacgctca cgatcggcga aaacagcaac gttcaggatg
     4501 gatctgttct tcacgccgac catggaatcc cgctggtcat cggggcgaat gtgaccgtcg
     4561 gccacatggt catgcttcac ggctgcacca tcggcgacaa cagcctgatc ggcatcggct
     4621 cgatcgtgct caatggcgcg aagatcggga aaaactgcct gatcggcgcc ggctcgctga
     4681 tcggcgaggg caaggagatc ccggacaaca gtctggtgat gggcgccccc ggcaaggtca
     4741 tccgcgaggt cggcgagcag caggcgatga tcctcaaggc ctcggcgctg cactacgtcg
     4801 agaactggaa gcgattccgg cgggagctgg cggagctcgg ttgacggacg gaggggaaac
     4861 tcttctccct tccccctgga ggggggaagg gtcggggatg ggggtggagg tgatcggccc
     4921 gggcgcggtt gatccgcaaa ggacctggtc gctcgccaac tcccttccaa ccgtccctgt
     4981 tcccaccccc atcccaaacc cttcccccct ccagggggaa gggcttcaga agaggaaaca
     5041 cccatggcct acgagttcat cacggtcgac cgcgaggggc cggtcaccat cttcaccctg
     5101 aaccggccgg aggtgatgaa cgccctccat tcgccgatgc atttcgagct ggacgaggcg
     5161 ttcaacgcct tcgccgccga tcccgagcag tgggtcggca tcgtcaccgg cgcgggcgag
     5221 cgggccttct cggccggcaa cgacctcaag caccaggccg gcggtggcgg catgggcacg
     5281 cctccgtcgg gcttcgcggg cctgaccagc cgcttcgacc tcgacaagcc gctgatcgcg
     5341 gcggtcaacg gcgtggccat gggcggcggg ttcgagatcg cgctcgcctg cgacatcatc
     5401 ctggccgccg agacggcgac gttcgccctg ccggaaccga aggttggcct ggccgccctg
     5461 gccgggggcc tgcaccgcct gccgcggcag atcggcctga agcgggccat gggcatgatc
     5521 ctgaccgccc gccgggtgcc cgccgcagag ggccaggccc tgggcttcgt caacgcggtg
     5581 gtcccggccg ccgacctgat ggccgaggcc cgcaagtggg cgcagatgat ctgcgagtgc
     5641 agtccgatgt cgatcagggc gagcaagcag gccgtcatgc agggcctcga ccagaccctg
     5701 cccgaagcca tcggcgggca gttcaagttt ccggccgtcg gcgccctgtt ccgctcggag
     5761 gacttcaagg aaggacccat ggccttcgcg cagaagcgcg cgccgcaatg gaaggggaaa
     5821 tagcaccgaa cccttctccc gttgcgggag aaggtgtcgg cgaagccgac ggatgagcgg
     5881 tttcaccagc gcaaacgaag aggggccgca accggctgac cgggtgcgac ccctcattcg
     5941 acccgccttg cgggccacct cctcccgcaa ggggagaagg atcacctcct acgaggcgct
     6001 ggccgccgcg ccgccgccgg agcgattgcg gttccggttg cgcggtttct tgcgcctggg
     6061 cggggcgtcg ccgccggccc ctgtcgccag gggctcgtag ccggccgggc ggtgacgcgc
     6121 gcgctggccg cgatcaggtt gggccttgcc ggatgcatcc agggcctgcg gcttgcgctt
     6181 ctgctgggcc gccttgaccg cccgcgggtc gcggtagggc ttctccggct cgaccgaggc
     6241 catggcggcc tgggcctggg ccaggccctt gtcgttgcgg cggtcccagg ccggaatggt
     6301 ctggcgggtg accttctgga tgtcgcgcag caggttgcgc tcgtcatcgg ccaccagggc
     6361 gatggccgag ccgttggcgc cggcgcgggc ggtccggccg atgcggtgga cataggcctc
     6421 cggcacgttg ggcagctcga actggatgac gtggctgacc ccgtccacat cgatgccgcg
     6481 ggcggcgatg tcggtggcca ccagggcgcg catcttgccg gccttgaact cggcgagggc
     6541 gcgttcgcgc tggccctggc tcttgtcgcc gtggatcgag gcggcctcga cgccgatggc
     6601 gttgagcccc ttggccacgc ggtcggcgcc gcgcttggtg cgggtgaaca ccagcacgcg
     6661 cttcatggcc gcctcgtcga acagttcggc cagcagcacg cgcttgcggg cggcttcgac
     6721 gaacagaacc ttctggtcaa tcttctcgac ggtggtcgcc tggggcgtca ccgagacctt
     6781 cgaggggttc gggttcagca gctcggcggc cagcttctcg atttccgagg gcatggtggc
     6841 cgagaagaac aggttctgac gaacgcgcgg cagggtcttg gcgatccggc ggatcggcac
     6901 gacgaagccc atgtcgagca tctggtcggc ctcgtcgagg acgaagacct cgacctccga
     6961 caggttggcg accttctcgt tgatgtggtc gatcaggcgg ccgggcgtgg ccaccagcac
     7021 atcgacgccg ccggcgaggg cgcgcacctg cgggccgtac ttcacgccgc cgaacacgac
     7081 ggcgacggaa aggccgaggt tggcgccata ggccttgaag ctctcgccga tctgggtggc
     7141 cagttcacgg gtcggcgaca ggaccaggca gcgacagccg cggcgcggag ccgggcgacg
     7201 gttctcggcc aggcggtgca ggatcggcag ggcgaaggcg gcggtcttgc cggtgcccgt
     7261 ctgggcgatg cccagcacgt ccttgccgtc catcaccagc gggatcgcct gcgactggat
     7321 cggggtaggg gtggtgtagc cctcggcggc cagcgcggag agcagcggct tggccaggcc
     7381 aagcgaagcg aaattggtca tggtctcttt cgtatgcgac tcgagccgca cggccatcgg
     7441 gcctgcggtc ggcagcgggt tcgtctggga aacgccccgc gtatggcgtc gctatgggga
     7501 agatcggtat cgagacccgg gcggaacctg tgggttccat acgcggccgg gcaggaccga
     7561 tagcgcggaa tgctccgcga cgaggcctat gtggcggtgc agcatgggaa agtcaaggaa
     7621 atggaccttc cgaagcgctt cacaccaccg acggccggtc caggtcatag acgtcggggc
     7681 ggctggcctc catcacgtcg agcagcgagc ggaacccctt tgtcttcaca tgctcgaact
     7741 cggtgtcgga gatcggaagg gccagcagcc aggccacggt ccggcccggc atcgtcaggg
     7801 tctgtggcct gtcgttccag tagaacggcg gcgtgaacag gatgtgccgc atggcgaggg
     7861 cggggtcgta cagcgccgtg acttcgggaa acaccacgcc cggcccgatg accgctccgt
     7921 gctcgatgac gttgaaggcg caggtcgaca gcaggctggg aaacagtgcg gtcgccgagc
     7981 gacaggcgcc cagcagttcg acccgcaggt cgttctcgac ctcccggagg gggtgattcg
     8041 acaggttcag ggtggcgtag gcggtcagtc cctcgccggg cgagtcgacg caggagagga
     8101 tgtcgacgct ggtcgtccgt ccgtcgtccc accaggtgaa gacctcgggc tggccgccga
     8161 aggtctcggc caccacccgg gcgatctcgc ggttttccgc cgtcaccggc gcgcgttcgg
     8221 tcatcgtctc accttgcaga tgcggctgcg cgcggttctc gccggcccag agggggtctt
     8281 tagcctcaga accgttcgcc gaccatctcc tcgctctttg accacagcgc ttcggcgcgc
     8341 gctgcgtcgc ggatgtagcc gcgcgcgccg ccgcgccctt cctcctgcgg ctcggcgacg
     8401 tggcagtcct cgcagtactg gccgccgatc tcgtcggcat cggccaccac ggcggcccag
     8461 acggtggtcg ccgcgccttg cgggatggtc ttgaattcga acgcgggcgc gccggtccgg
     8521 gcattcgcct cggtgatcga cttcatcatg cccgccgcca gttccggcgt catgtgccgg
     8581 cccagctcgg tctggatgcc gccgggatgg acggaggcgg cgcgcacgcc acgccccctg
     8641 ttccgctggt cgaaggccag ggcgaacagg gcgttggcgg tcttgctgcg gccgtaggcg
     8701 ccgaactcgg tgtagggcgt gcgctcgaag ttggggtcgt caagatccac gtcggagaac
     8761 cggtgccccg ccgacgacag gcagatcacg cgcccgccgg actggagcag cgacgcgatg
     8821 cggttgacga acacgaagtg acccagatgg ttggtgccga actgggtctc gaagccgtcg
     8881 acggtcctgc cgaacggaca ggccatgacg ccggcgttgc agatgaccac gtcgaacggc
     8941 cggccggccg cgaccagggc gtcggcgcag gcgcgcacgc tggccaggga agcgaggtcc
     9001 atcgcgacca gctcgatgcc gggaattccc gcggtggcgg tccgggcctt ggccaggtcc
     9061 cgcgcggtcc cgatcacctg ggccccgtgg gccgcaaggg ctcgcgccgt ctcgacgccc
     9121 agccccgcgg acacgccggt caccagaatg cgtttgccgc ccaaggccac gcccgaaagg
     9181 acgtcgtcgg ttgtcgacgt cgcgccgaaa gtcatgatgt cttctccagt ctgctgtcgt
     9241 cagagacccg cgccgccgac aggcgccgtc tcgatccgct cgggcccgaa gccggcgatg
     9301 atgggccggg ccctggcgat ggcggcctgg acctccggca gcttcagcga gtctgcgtga
     9361 tgctggcggc tggcccagat ctcgttgatc catacagcgt ccgcgtccgt cggatccctc
     9421 gccaccacat agagcaggca tcccggcatc gcgcccgttc cggccagcag atagccgatc
     9481 agttcgtccc gcttcccggg cgcggcgacc agcttgccga acaggccgta cttctcggtc
     9541 tcatcgccca tgacgacctc cggaatcagg gttgcgagcg cggctgcggt ggcggcgcgg
     9601 cgagtaagca tgccggaccg tcgcatggcg cctcgcacct gtcatcccgg acggcgtggt
     9661 cgatccgggt cccggctgtc cggtttactg tggccgggat ggcaaccgga ctggcggaac
     9721 agcgatctcc caacatgaac gaacggtaac cttcaccctc caagccggtt caggaaaggc
     9781 cgcgcagtct cgacctcagc gaaatccttc gcggggctaa caggagacta ccatgttcag
     9841 gaccatcgtt tcagtcgcca gcgttgtcgc caccgtttcc gtcgcggcgt ccgccatggc
     9901 cggacccatc acggccaagg gcttcacccc ggtccacagg ctgcccgagg gcgcggcgcc
     9961 cgtcgccgtc tggacctgtg atgtcgaccc ctcgatcgtc ggcgtgaccc tgaccaaggg
    10021 cgcgcgccgc ggggaggtcc ggatcagcta cgagatcgcc aacaccggcg gctcggcctg
    10081 gcgctcgggc gccaaccagc agggcgtcca cctggtggcc gtcaacggca acaccggcgg
    10141 cgcctacgcc agcgaccggc cgttgaccgg cgccgccgcg ccgggggcga ccatgcaacg
    10201 gttcaccacg ccgctgatcc gcaacgcctt cgacgacttc gagttcggcg gccacgtcgc
    10261 gctgagcatc agctacgacc ccgacatctt catcgacggc aaccgctgca acgacgactc
    10321 gaacgccggc aacaacacct tccgcatcga gaccggcgag attctcgcct tcatgaacgg
    10381 cgcggccacg agccggacct tccgtccgta gcctggttcg cgacgatcgt gagaggggag
    10441 ggccacacgg ccctcccttt tctcgttgcc caatcctgac gcgggcgtca ctaatccctg
    10501 aacaacgata acctgctccg ggagacgcca ggtgggcgac cgctacgact acatcatcat
    10561 cggcgccggc tcggccggtt gcgtgctggc caaccggctg accgagggcg gtgacaggac
    10621 cgtcctgctg ctggaggccg ggggcaagga caactccatc ctggtcaaga tgcccgccgg
    10681 cgtgggcagc ctgatcaagg acaagggaac ctacaactgg ggcttctgga ccgaggcgga
    10741 gccggccctc aacgaccgca agctgtggtg gccgcgcggc aagggctggg gcggctcgtc
    10801 ctcgatcaac ggcatggtct atatccgcgg ccatgcgcgt gactacgacc agtggcggca
    10861 gatgggcctg accggctggg gctacgctga cgtgctgccc tatttcaagc gctcggagag
    10921 cctcgagggt ggcggcgacg cctggcatgg cgacaagggc ccgctgcacg tctccaaggc
    10981 cgcctcgccc aacccgatct attccagcgt catcgaggcg ggccgccagg ccggctatcc
    11041 cgtgaccaag gacttcaacg gctaccagca ggaaggcttc ggcccctacc agttcaccat
    11101 caaggacggc gagcgctgga gttcggccaa ggcctatctc tatccggccc tctcccggaa
    11161 aaacctgacc tgcgtcaccg gcgcccgcac gagccgcatc gtcatcgagg gcggccgggc
    11221 gacgggcgtg gagtacctgg tcgatggcgc gtccgtgacg gcccacgcca acgccgaggt
    11281 gctgctctgc gccggcgccg tgcagtcgcc gcagatcctg cagctgtcgg gcatcggcga
    11341 cccggaggac ctgaccccgc acggcatcaa ggtcgtgcat gcgcttccgg gcgtcggcaa
    11401 gaacctgcag gaccacctcg acgtcaccct cagctggctg acccggggcg tgcagaccgc
    11461 ctacagctac aacaagggct tcaagcagct gctgaccggc ctcaactacc tgctgttcaa
    11521 gaagggcttc ggtcggcaga acttcctcga atcgggcgcc ttcctgcgct cgcggcccga
    11581 cctcgaccgg ccggacctgc agatccacac cgtgctggcg atcatgcaga accacggcaa
    11641 gaccgtgatc gagaaggacg gcttcaccct gcatgtctgc cagctgcggc cggagagccg
    11701 cggcagggtc ggcctgcggt cggccgatcc caacgacgat ccgaccatct tcgccaacta
    11761 cctggccacc gaggaagacc gccgcgcgat ccgcgagggc gtcaagatgg cccgccaggt
    11821 ggccgcccag tcggcgctca ggccctatat cgaggaagag ttcgaacctg ggccggcggt
    11881 gcggaccgac gatgagatcg acgcctggat cagggccaag gccgaaacca tctaccaccc
    11941 ggtgggcacc tgccgcatgg gcgtggccgg cgacgcccag gccgtggtgg acgccgagct
    12001 gaacgtcatc ggcatccagg gcctgcgcgt ggtcgacgcc tcggtcatgc cgaccctgat
    12061 cggcggcaac accaatgcgc cgacgatgat gatcgccgag aagatcagcg acgtgatcct
    12121 cggcaagccc gccctgccgc cgcaggacgc gccggtccat gacggcgagg ccgtgaaggc
    12181 ggcgtgattt tccgctcgcc ccttctcccc ttgcgggaga aggtggccgc gcagcggccg
    12241 gatgaggggt tgatgatccc gtcaacctcg gagtcggcgc tgctatccga cggggtcgcg
    12301 gcgacccctc atccgtcagg cttcgcctga caccatctcc cgcgagggga gagggacaag
    12361 gagcgaggca tggaacaccg tcctctcggc cgctcgggcc tggccatcgc cccgctgatg
    12421 ctcggcggca acgtgttcgg ctggacggcg gacgaggcga cctcgcacgc catcctcgac
    12481 gcgttcgtcg ccggcggctt caacgccatc gatacggccg acgtctattc agcctgggtt
    12541 ccgggcaaca cgggcggcga ttcggaaaag gtcatcggaa cctggctcag tcgacgccgt
    12601 cggcgcgacg acgtggtcat cgccaccaag gttggcatgt ggccggtcca gccggggctg
    12661 aaggccgcca acatcgtcgc cgcctgcgaa ggctcgctgc agcggctgca gaccgaccac
    12721 attgacctct accagtccca tcgggacgac gccgatacgc cgcaggacga gacgctcgag
    12781 gcctacgacc ggctgatcaa ggcgggcaag gtgcgggcca tcggggcgtc gaacttcgag
    12841 acggcgcgac tggattcggc gctcgacatc gcccgggcga aaggcctgcc gcgctacgac
    12901 accctgcagc cggcctacaa cctgatggac cggagcgtgg agggcccgct gcagcagctg
    12961 tgcctcgacg aggacgtcag catcatcagc tactacggcc tggccagcgg cttcctgacc
    13021 ggcaagtacc ggtcgtcggc ggacctgggc aagagcccgc ggggcggcgg cgtcaagaag
    13081 cacctcgaga gcgaacgggg gccgaaggtg ctggccgcgc tcgacgcggt gtcccaggcg
    13141 cttggcgcga ccccggccca ggtcgccctg gcctgggtca tggcccggcc ggcggtcgcc
    13201 gccccgatcg ccagcgccac cagcctggaa caactcgagg aactgatggn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnncgctt tcgccgcgaa gtatcccgcc gagggcgagc gcaagggccg
    13441 gacggccgag caggcgggcg atcatcgcgg tgggctgata caggccgatg aagcggtcga
    13501 cgtcgcccct cggtccacgc agcggggcca gcaggacctc catgccgacc ggcgcgacgc
    13561 cgtcggcgag gatgtcggcc ttgaccacca gcggctcggg t
//
LOCUS       DAORNX010000019        13544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3434_length_13544_cov_6.655868, whole genome shotgun sequence.
ACCESSION   DAORNX010000019 DAORNX010000000
VERSION     DAORNX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_13544_cov_6.655868"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLO65_01605"
     CDS             complement(<1..471)
                     /locus_tag="PLO65_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRD26975.1"
                     /translation="MTGFARKLAVSAVITAILGGAAGAQSMQPEGGPQLVTTPQIAYR
                     PSLSDSDAAYLSTALTVARAGDGSRARLAMDSIQDPVARKIALWALADGAPDSLSFFE
                     ADSVRRDLNGWPRAARRQATAEKLLETSGMSPDRIIAWFGGAEPATVQGAMALAG"
     gene            597..1481
                     /gene="dapA"
                     /locus_tag="PLO65_01610"
     CDS             597..1481
                     /gene="dapA"
                     /locus_tag="PLO65_01610"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRD26976.1"
                     /translation="MVQFPFRGALPALVTPFRDGAVDEAAFVALVERQIAGGVHGLVP
                     VGTTGETATLSHDEHRQVVELCVRTARGRVPVIAGAGSNATAEAIELVRHAKTVGADA
                     ALCVTPYYNRPSQEGLYEHYRAINDAVQLPVLVYNVPSRTAVDISNETLARLSVLPNI
                     LGVKDATGDMSRVSFQRLMCGDEWLMLSGDDPSALGYMAHGGHGCISVTANVAPDGCA
                     AFFNAAMAGDWDTARYWQDRLVRLHKALFLDASPAPAKFALAHLGLCSGDCRLPITPC
                     AEAVKPAILDAMHEAGVI"
     gene            1490..1948
                     /gene="smpB"
                     /locus_tag="PLO65_01615"
     CDS             1490..1948
                     /gene="smpB"
                     /locus_tag="PLO65_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRD26977.1"
                     /translation="MGRAIAENRRARFDYFIEDTVEAGIILTGTEVKSLRNGRANIAE
                     SYAAVEGDEIVLINADIPPYSGGNRFNHEPRRPRKLLLHRKQIGKLIGAVQREGRTII
                     PTKLYWNEKGIAKLEIGLAKGKKLHDKRETSAERDWQRDKARLMKGDRTG"
     gene            2017..>2512
                     /locus_tag="PLO65_01620"
     CDS             2017..>2512
                     /locus_tag="PLO65_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD26978.1"
                     /translation="MSEIGSRLSRSSSGPRVARLGLTVAALVFVLVGGGCTVTACSTT
                     VESGEAGIKTTKFGRNPGVQPTELRPGWHWKGIGEDIIIYPTRQRTYSYTREANSDGK
                     ENEEIAFADQTGLPMTADVNLTIRVRDDRAAELYARWRQPFDDLLDGQIRNDVRSAIA
                     KETEQ"
     assembly_gap    2513..2536
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2537..3010
                     /locus_tag="PLO65_01625"
     CDS             <2537..3010
                     /locus_tag="PLO65_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD26979.1"
                     /translation="PVATGPGVAPTPAASTAAPTPCTGSLMGAGRQLVIQRAFQRVRS
                     KWAAEGVEISQMEWVGTIRYPPSVTAAVEARTQTEQRTLAAQQKAAEARANAEAAIET
                     ARGIAESTRLRGEALRANPEIIQQIYAERSKGLCPPGTETCIIGQGAWGLVPGKE"
     gene            3248..3622
                     /locus_tag="PLO65_01630"
     CDS             3248..3622
                     /locus_tag="PLO65_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26980.1"
                     /translation="MLIVRFAAAPVAALALIAGPAAAGELYGDSFLGSPTHWTIDGTP
                     LMSVPAKGTSAATVTGGAHSIDVTSDNGFRLIETIVLDESQLADVRGKQYWCVMIATE
                     GYRMFSVLLDREACQGLVEAAK"
     gene            complement(3633..4268)
                     /locus_tag="PLO65_01635"
     CDS             complement(3633..4268)
                     /locus_tag="PLO65_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRD26981.1"
                     /translation="MSPRSEPPRDCALCPRLVEYRRENQRQNPDWWNGPAPSFGDPAA
                     RLLIAGLAPGRTGANRTGRPFTGDHAGWLLYDTLRKTGFATGTYDPNGDDDLRLTGAM
                     ITNVVRCAPPQNKPETSEEKTCSPFFAAKLEELTNLKVIVTLGDVARRSVLRAMGLKA
                     SLGEPGHGSQFQAGPYAVINSYHCSRLNTNTGRLTPAMFEDIFVRARGLLS"
     gene            complement(4272..4880)
                     /locus_tag="PLO65_01640"
     CDS             complement(4272..4880)
                     /locus_tag="PLO65_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRD26982.1"
                     /translation="MTFYPTDRLALFIDGANLYSQAKALGFDIDYRKLLDEFRKRGVL
                     IRAYYYTALAESGDDFSPLRPLVDWLDYNGFTVVTKPAREYTDAQGRKRWRGDMDVEI
                     AVGMMEIAGKADHLVLFSGDGDFRALVEGVQRKGARVTVVSTVKSQPPMVSDDLRRQC
                     DAFVDLADLGDIIGRPQRTHQPRFLNDPPARTRAEVEADDEI"
     gene            4893..5420
                     /gene="folK"
                     /locus_tag="PLO65_01645"
     CDS             4893..5420
                     /gene="folK"
                     /locus_tag="PLO65_01645"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRD26983.1"
                     /translation="MNAISQTASDESIVIALGGNLAGAYGDCEALLEAALAEFARHGL
                     TVTARSGWWRSAAWPDPTLPDYINGVALVETALPPGEVLQALHAIERDFGRVRGEANA
                     ARTLDLDLIAFGRLVLDQPGLHVPHPRAHERLFVMGPLAEIAPDWRHPVSGETAAALA
                     VKASVGADAVSLTPT"
     gene            complement(5374..5841)
                     /locus_tag="PLO65_01650"
     CDS             complement(5374..5841)
                     /locus_tag="PLO65_01650"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRD26984.1"
                     /translation="MTDRWPDPETALNGFLEKFDPDIAADARACMQRLRGRLPMADAM
                     IYDNWNGLVMGFSPNDRPSDAVLSILAVAGHVTLCFLQGARMKDPYGLLKGAGTTVRH
                     VRLKGPAELDDPEISHLIDLALTGAKVPMVEGRNGRMYVKSVSAKQRPRRRMP"
     gene            complement(5838..6143)
                     /locus_tag="PLO65_01655"
     CDS             complement(5838..6143)
                     /locus_tag="PLO65_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26985.1"
                     /translation="MIKVLMERPLPADFDVLDDAQAAWHARVALDSINASNGAMRWLC
                     TYATEDRKLFGQVEVESEAVIDDFRKRAGIGTGILVHKVLRVIDAATAGPPGMTKPA"
     gene            6230..6589
                     /gene="rpoZ"
                     /locus_tag="PLO65_01660"
     CDS             6230..6589
                     /gene="rpoZ"
                     /locus_tag="PLO65_01660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRD26986.1"
                     /translation="MARVTVEDCVEKIPNRFGLVLVAAHRARAISAGSALMVDRDNDK
                     NPVVALREIADDVVDNDGLKESLIGTLQRVDERTEAEEEAETVALLSDPTHMQMSEQE
                     LIRALQSDRDGGQEERY"
     gene            6652..8886
                     /locus_tag="PLO65_01665"
     CDS             6652..8886
                     /locus_tag="PLO65_01665"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRD26987.1"
                     /translation="MNTPPAAPDAAPHAVARAKAAAPKFLRQYELIERVRAYDPTADE
                     GLLNRAYVYAVRMHGSQTRASGDPYFAHPIEVAGILTEYRLDTASIVTALLHDVIEDT
                     HASSADISELFGPEVAELVEGVTKLSKLELAAEHARQAENLRKFILAISKDVRVLMVK
                     LADRLHNMRTLGFIKSPAKRERIARETLDIYAPLARNIGCHRICTELEELAFEHLNPV
                     ARNAILRRLEAMRVDQGKAVQQVSGDISRILEAESIPARVFGREKHPFSIWRKLQRKS
                     IGFSQMSDIYAFRVIVDSEADCYRALGLIHRQWPCVPERFKDFISTPKRNNYRSLHTT
                     VIGPKGMRVEMQIRTEVMDRVAEDGVAAHWRYKNKSYGFDREAMEAEGGRDPLANLRQ
                     LVQMLEHGGDSEDLVEHAKMELFLDQVFVFTPKGKLITLPRGSMPLDFAYGVHTNVGD
                     TTVGAKINGELKPLRTELQNGDVVEIVRGPKPAVLPDWRSLTVTGKARSAIRRHIRQT
                     EKEEFLRLGVAAMDQAFARVGKTRSGVTLRPALDRFAVAGEDELFDAIGRGRITPAEV
                     LETIFPGLKDSEKAAVTARRRITDGKEARDFVIGDRLRAGTLLRFATCCRPVPGDRIV
                     GIAVDKGVEVHAIDCPTLEQYDDRQDLWRDLHWTAEAERNTVSRTRLRATIRNAPGVL
                     GEACTIIGEVGGNIVNLNMHHRGDDHFDVDFDLDVKDARHATNIVAALRANPSIETVE
                     RAKG"
     gene            complement(8926..9333)
                     /locus_tag="PLO65_01670"
     CDS             complement(8926..9333)
                     /locus_tag="PLO65_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD26988.1"
                     /translation="MTTPSPVPRVGCGAAILRDGKLLLVQRLKQPEALYWGLPGGKVD
                     WGEDTRATCAREIAEELGLTITPGRLLCVADTIDLGDGQHWVAPVYLIEDCLGEPVVL
                     EPDKLGGCGWFALDALPAPLTSATVAAVKALTK"
     gene            9506..10087
                     /gene="pyrE"
                     /locus_tag="PLO65_01675"
     CDS             9506..10087
                     /gene="pyrE"
                     /locus_tag="PLO65_01675"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRD26989.1"
                     /translation="MTQDDVLDEFRGAGALREGHFVLSSGLHSGVFLQKNLVFAQPDR
                     CERLCRALAAKIVAEVGKPDVAISPAVGGIIPGYETARHLGVQSLYVEREGGAFKLRR
                     GFSIEPGAKVVMVEDIVTTGLSSRECIAAIREAGGEVIAAACIVDRSGGRADVGVPLI
                     ALATLDVPAYEADSLPPELAAIPVQDPGSRRLR"
     assembly_gap    10152..10173
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10215..10976
                     /locus_tag="PLO65_01680"
     CDS             10215..10976
                     /locus_tag="PLO65_01680"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRD26990.1"
                     /translation="MNRLRLGVNIDHVATIRNARGAGYPEPSRAAEMALAAGADGITA
                     HLREDRRHISDADIDILADLCLKRGKPLNFEMAVTDEMVGIALRNRPHAACLVPERRE
                     EVTTEGGLDVAGGHNRIAPAVERLRSAGCRVSLFIEADPAQIETAAKIGAQVVELHTG
                     AFCDAFRDGDHDRAGVILKRLRDGAALAHELGLEVHAGHGIDYATVAPVAAIPELMEL
                     NIGHFLIGEAIFIGLAPAIQRMRVLMDAARAELAA"
     gene            10973..11392
                     /gene="acpS"
                     /locus_tag="PLO65_01685"
     CDS             10973..11392
                     /gene="acpS"
                     /locus_tag="PLO65_01685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRD26991.1"
                     /translation="MIVGIGSDLSDIRRIEKTLERFGERFINRTFTAIERDRSERKVN
                     AQGGRAASYAKRFAAKEACAKALGTGVPRRGVHWADMGVINLRSGKPTMALTGGAAER
                     LAEITPEGMVAVIHLSLTDDHPYAQAFVIIEAVPPAG"
     gene            11481..12281
                     /gene="lepB"
                     /locus_tag="PLO65_01690"
     CDS             11481..12281
                     /gene="lepB"
                     /locus_tag="PLO65_01690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRD26992.1"
                     /translation="MTDAVESPDDDSAPAPADPAARRAELVETLTTVAYALLIALVLR
                     VLIFQPFTIPSSSMRPNLLIGDYVVVSKFSYGYSRHSIPFSPPLFHGRIFAGTPRPGD
                     VIVFKTPATGHKDLIKRLIGLPGDTIQVKAGALYVNGRALPRVADGLATGDEAACPNG
                     ATPLRFLETNPIGRRYVTYDCGLGPLDNTPVFRVPAGHYFFMGDNRDNSLDSRVSALA
                     GGVDYVPAENLVGKAETVMVSWREGASLFKPWTWVLNLRWNRFFHSLR"
     gene            12419..13255
                     /gene="lepB"
                     /locus_tag="PLO65_01695"
     CDS             12419..13255
                     /gene="lepB"
                     /locus_tag="PLO65_01695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRD26993.1"
                     /translation="MTDAAEPQVETVADAEPLALERRHDDGQEQTGGGAVGETIEIVK
                     TVVYALLIALVLRVFLFQPFTIPSASMEPNLLKGDYIIVSKYSYGYSRHSIPFSPPLF
                     KGRIFSRAPERGDIIVFKLPSDNHTDYIKRLIGLPGDRIQVKGGVVSVNGKVLTRVYD
                     GPGDPGTCWNGSTVQRFKETNPIGRTYITYDCGPRGDVDDTGVYVVPEGHYFFMGDNR
                     DNSLDSRVAPEASGVGFVPAENLVGHARIILLSWNGDAALFKPWTWFLDARPSRFFHV
                     LK"
     gene            13252..>13544
                     /locus_tag="PLO65_01700"
     CDS             13252..>13544
                     /locus_tag="PLO65_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRD26994.1"
                     /translation="MNARLEAVAALEQRIGYVFTDRVLLERALTHASVGDGARKVTHN
                     ERLEFLGDRVLNLLAAERLLSLDPEAKEGVLSPRLAALVNGKACARVARRIGLP"
BASE COUNT         2185 a         4742 c         4550 g         2021 t
ORIGIN      
        1 gccggccagg gccatcgcgc cctgcacggt cgccggctcg gcgccgccga accaggcgat
       61 gatccggtcg ggcgacatgc cggaggtctc gagcagcttt tccgctgtgg cctggcgccg
      121 ggccgcgcgc ggccagccgt tcaggtcgcg acggaccgaa tcggcctcga agaaactgag
      181 ggagtctggc gcgccatcgg ccagggccca gagggcgatc ttgcgcgcca ccggatcctg
      241 gatggaatcc atcgccagac gggcgcgcga cccgtcgccg gccctggcca cggtcagggc
      301 cgtgctcaga taggccgcgt cgctgtcgga cagcgacggc cggtaggcga tctggggcgt
      361 tgtcaccagc tgcggaccgc cctcgggctg catcgactgg gcgcccgcgg cgccgccgag
      421 aatcgcggtg atcaccgcgc tgaccgccag ctttcgtgca aatcccgtca aaccatcgtc
      481 tccaagccat caaacccggc gcgcggcggt tgcggtcgcc ccgtgcgggg acataatgtc
      541 cgccaccgac acacagccgc aaacgaaagc ggccatctca cggctttttg caagtcatgg
      601 tccagtttcc cttcaggggc gccttgcccg cccttgtgac tccctttcgc gacggcgcgg
      661 tcgacgaggc cgcgtttgtc gccctggtcg agcggcagat cgccggcggc gtccacggcc
      721 tggtgccggt cggcaccacc ggcgagaccg cgaccctgag ccatgacgag caccggcagg
      781 tggtcgagct gtgcgtgcgg accgcccgcg gccgcgtgcc ggtgatcgcc ggggccggca
      841 gcaacgccac ggccgaggcg atcgagctgg tgcgtcacgc caagacggtc ggcgccgacg
      901 cggccctgtg cgtcacgccc tactacaatc gccccagcca ggaggggctc tacgagcact
      961 accgggcgat caacgacgcg gtgcagctgc cggtgctggt ctacaatgtg ccctcgcgca
     1021 cggccgtgga catcagcaac gaaaccctcg cccggctgtc ggtcctgccc aacatcctcg
     1081 gcgtcaagga cgccaccggc gacatgtcgc gggtcagctt ccagcggctg atgtgtggtg
     1141 acgaatggct gatgctgtcg ggcgatgatc ccagcgccct cggctacatg gcccacggcg
     1201 gccatggctg catctcggtg acggccaatg tcgccccgga tggttgcgcg gccttcttca
     1261 acgcggccat ggccggggac tgggacacgg cgcgctactg gcaggaccgg ctggtgcggc
     1321 tgcacaaggc gctgttcctc gacgcctcgc cggcgccggc caagttcgcc ctggcccatc
     1381 tgggcctttg ctccggggac tgccgcctgc cgatcactcc ctgcgccgag gcggtcaagc
     1441 cggcgatcct cgacgccatg cacgaggcgg gcgtcatctg atgacccagg tgggccgggc
     1501 catcgccgag aaccgccgtg cgcggttcga ctacttcatc gaggacacgg tggaggccgg
     1561 catcatcctg accggcaccg aggtgaagag cctgcgcaac ggccgggcca acatcgccga
     1621 gtcctacgcg gcggtggaag gcgacgagat cgtcctgatc aacgccgaca ttccgcccta
     1681 cagcggcggc aaccgcttca accacgagcc ccgtcggccg cggaagctgc tgttgcaccg
     1741 caagcagatc ggcaagctga tcggcgcggt ccagcgcgag ggccgcacga tcatcccgac
     1801 caagctctac tggaatgaga aaggcatcgc caagctcgag atcggcctgg ccaagggcaa
     1861 gaagctgcac gacaagcgcg agaccagcgc cgagcgcgac tggcagcgcg acaaggcccg
     1921 cctgatgaag ggcgaccgga cggggtaggg cgactcatgg cctgatgccc gccatgaggg
     1981 cggcggacgg accgccgccc gaccggggag gcccgcatga gcgaaatcgg cagccgtttg
     2041 tcacgatcgt cctccggccc ccgggtggcg cgcctggggc tgaccgtcgc cgccctggtg
     2101 ttcgtgctgg tcggtggcgg ttgcacggtc acggcctgca gcaccacggt cgagagcggc
     2161 gaggccggca tcaagaccac caagttcggc cgcaatcccg gcgtccagcc aaccgagctg
     2221 cggccaggct ggcactggaa gggcatcggc gaggacatca tcatctatcc gacccgccag
     2281 cggacctaca gctatacgcg cgaggccaat tccgacggca aggagaacga ggagatcgcc
     2341 ttcgccgacc agaccggcct gccgatgacc gccgacgtca acctgaccat ccgcgtgcgc
     2401 gacgaccggg ccgccgagct ctacgcccgc tggcgccaac cgttcgacga cctgctcgac
     2461 ggccagatcc gcaacgacgt gcgctcggcc atcgccaagg agaccgagca ggnnnnnnnn
     2521 nnnnnnnnnn nnnnnnccgg tggccaccgg accgggcgtc gcgccgacgc cggccgcctc
     2581 gaccgccgcc ccgaccccct gcaccggctc gctgatgggc gccggccgcc agctggtcat
     2641 ccagcgcgcc ttccagcgcg tgcgcagcaa atgggccgcc gagggcgtcg agatcagcca
     2701 gatggaatgg gtcggcacca tccgctatcc gcccagcgtc accgccgcgg tcgaggcccg
     2761 cacccagacc gagcagcgca cccttgccgc ccagcagaag gccgccgagg cccgggccaa
     2821 cgccgaggcg gccatcgaga ccgcccgcgg catcgccgaa agcacccgcc ttcgcggcga
     2881 ggccctgcgc gccaatcccg agatcatcca gcagatctac gccgaacggt cgaagggcct
     2941 gtgtccgccc gggaccgaga cctgcatcat cggccagggc gcctggggac tggtgccggg
     3001 caaggagtag gaggacggcc agggggcagg aacggtaggg ggacaggctg aactgccccc
     3061 tggtgtccct tgggacagct tgacctgtcc ccgtctccat gactctcccg ctggcaatcc
     3121 gggcggcgcc ggacaactgc cggaaggcgg ttcgaaagat ggtcgtccag gggcgtccgt
     3181 aggcatcgcg cgggcttggc attctgcgag gatcgccgat actgggtcgc gaagggggag
     3241 gcccgtcatg ctgatcgtcc gtttcgccgc ggcccccgtc gccgccctgg ccctgatcgc
     3301 cggtcccgcg gcggccggcg agctgtacgg cgacagtttc ctgggctcgc cgacccactg
     3361 gaccatcgac gggacgcccc tgatgtccgt cccggcaaag ggcacgagcg ccgcgaccgt
     3421 tacgggcggc gcccattcca ttgatgtcac gtccgataac ggctttcgcc tgatcgagac
     3481 gatcgtgctg gacgaatctc aactcgccga cgttcggggc aagcaatact ggtgcgtcat
     3541 gatcgcaacc gaaggctatc ggatgttttc tgtgctgctg gaccgcgagg cctgccaggg
     3601 ccttgtcgag gcggctaaat aggcggccag gctcagctca acagcccccg cgcccgcacg
     3661 aaaatgtcct cgaacatcgc cggcgtcagc cggccggtgt tcgtgttcag ccgcgagcag
     3721 tgatagctgt tgatcaccgc gtagggtccg gcctggaatt gcgagccgtg accgggttcg
     3781 cccagcgagg ccttcagtcc catggcccgc agcacgctgc gccgggccac gtcgccgagg
     3841 gtgacgatga ccttcagatt ggtcagctct tccagcttgg ccgcgaagaa cgggctgcag
     3901 gtcttctctt ccgacgtctc gggcttgttc tgcggcgggg cgcagcggac cacgttggtg
     3961 atcatcgccc cggtcagccg caggtcgtcg tcgccgttcg gatcataggt cccggtggcg
     4021 aagccggtct tccgcagggt gtcgtagagc agccagccgg catggtcgcc ggtgaacggc
     4081 cggcccgtgc ggttggcccc ggtgcggccg ggggccaggc ccgcgatcag cagccgcgcg
     4141 gccgggtcgc cgaaagacgg ggcagggccg ttccaccagt cgggattctg ccgctggttc
     4201 tcgcggcggt attcgaccag gcgcgggcag agcgcgcagt cgcgcggcgg ctcggagcgc
     4261 gggctcatgg ctcaaatctc atcgtcggcc tcgacctcgg cgcgggtgcg ggcagggggg
     4321 tcgttgagaa agcgcggctg atgcgtccgc tgcggccggc cgatgatgtc gcccaggtcg
     4381 gcgaggtcga cgaaggcgtc gcactgtcgg cgcaggtcgt cgctgaccat cggcggctgc
     4441 gacttgacgg tcgagaccac ggtcacccgg gcgcccttgc gctggacccc ttcgaccagc
     4501 gcccggaagt cgccgtcgcc gctgaacagc accaggtggt cggccttgcc ggcgatctcc
     4561 atcatgccga ccgcgatctc gacatccatg tcgccgcgcc agcgcttgcg gccctgggcg
     4621 tcggtgtatt cgcgggccgg cttggtcacc acggtgaagc cgttgtagtc gagccagtcg
     4681 accagcggtc gcagcgggga aaagtcgtcg ccgctctcgg ccagggcggt gtagtagtag
     4741 gcccggatca gcaccccgcg cttgcggaac tcgtccagca gcttgcggta gtcgatgtcg
     4801 aagcccagcg ccttggcctg ggaatagagg ttggccccgt caatgaacag ggcgagcctg
     4861 tcggtcgggt aaaacgtcat gttttcaaat ccttgaacgc aatatctcaa acagcatcgg
     4921 acgaatccat cgtcatcgcc ctgggcggca atctagccgg ggcgtacggc gactgcgaag
     4981 ccctgctcga ggccgcgctg gccgaatttg cgcgtcacgg cctgacggtg acggcccgct
     5041 cgggctggtg gcgctcggcg gcctggccgg acccgacgct gcccgactac atcaacggcg
     5101 tggccctggt ggagaccgcg ctgccgccgg gcgaagtcct gcaggccctg cacgccatcg
     5161 aacgggactt cggccgagtg aggggagagg ccaacgcggc gcgcaccctg gacctggacc
     5221 tgatcgcgtt cggccgactg gtcctcgatc agcccggcct ccatgtgccg catccgcgcg
     5281 ctcacgagcg cctgttcgtc atggggccgc tggcggagat cgcgccggat tggcgccatc
     5341 cggtgagcgg cgagacggcc gccgccctcg ccgtcaaggc atccgtcggc gcggacgctg
     5401 tttcgctgac accgacttga cgtacatccg gccgttgcga ccctcgacca tcgggacctt
     5461 cgccccggtc agggccaggt cgatcagatg cgagatctcc ggatcgtcca gctcggccgg
     5521 ccccttcagc cgcacatgcc gcacggtcgt gccggcgccc ttcagcaggc cgtagggatc
     5581 cttcatcctc gcgccctgca ggaagcagag ggtcacgtgg ccggcgaccg ccaggatcga
     5641 cagcaccgcg tcggacggcc ggtcgttggg cgagaacccc atcaccaggc cgttccagtt
     5701 gtcgtagatc atcgcgtcgg ccatcggcag gcggccccgc agccgctgca tgcatgcgcg
     5761 agcgtcggcg gcgatgtccg ggtcgaactt ttcgaggaag ccgttgagcg cggtttcggg
     5821 atcgggccag cggtcggtca tgccggcttc gtcatccccg gcgggccggc cgtcgcggcg
     5881 tcgatcaccc gcagcacctt gtggaccagg atgccggttc cgattccggc ccgctttcgg
     5941 aagtcgtcga tcaccgcctc gctctcgacc tccacctgcc cgaacagctt gcggtcctcg
     6001 gtggcgtagg tgcagagcca gcgcatggcg ccattcgagg cgttgatgct gtcaagggcg
     6061 acgcgggcgt gccaggcggc ctgggcgtcg tcgaggacgt cgaagtcggc gggcaggggc
     6121 cgctccatga gaactttgat catgtaacct ccttcgcagc cttgcggcgt tgcacaatga
     6181 cgcgcaaaac tctatgtacc ccggtcccac cggaagctga gaatcccaaa tggcccgcgt
     6241 caccgtcgaa gattgcgtcg agaagatccc caaccgcttc ggtctggtcc tcgtcgccgc
     6301 gcaccgcgcc cgcgccatct cggccggctc ggctctgatg gtcgaccgcg acaacgacaa
     6361 gaacccggtc gtcgccctgc gcgagatcgc cgacgacgta gtcgacaacg acgggctgaa
     6421 ggaaagcctg atcggcaccc tgcagcgggt ggacgagcgg accgaggccg aggaagaggc
     6481 cgagaccgtc gccctgctgt cggatccgac ccacatgcag atgagcgagc aggaactgat
     6541 ccgcgcgctg cagagcgacc gcgatggcgg tcaggaggag cggtactgag cccggacggc
     6601 acagaccctc ttgaactgat cgaggcgacg acacccgccg ccgcccctcc catgaacacg
     6661 cctccggcgg cgcccgatgc ggcgccgcat gccgtcgcgc gggccaaggc cgcggcgccc
     6721 aagttcctgc gccagtacga gctgatcgag cgggtcaggg cctatgatcc gaccgccgac
     6781 gagggcctgc tgaaccgggc ctatgtctat gccgtccgca tgcacggctc gcagacccgg
     6841 gcctccggcg acccctattt cgcccacccg atcgaggtgg ccgggatcct caccgagtac
     6901 cggctcgata cggcctcgat cgtcaccgcc ctgctgcacg atgtgatcga ggacacccac
     6961 gcctcgagcg ccgacatcag cgagctgttc gggccggaag tggccgagct ggtcgagggc
     7021 gtgaccaagc tgtccaagct cgagctggcc gccgaacacg cccgccaggc ggagaacctg
     7081 cgcaagttca tcctggccat ctccaaggat gtgcgcgtcc tgatggtcaa gctggctgac
     7141 cggttgcaca acatgcgcac cctgggcttc atcaagagcc cggccaagcg cgagcggatc
     7201 gcccgcgaga cgctcgacat ctacgccccg ctggcgcgca acatcggttg ccaccgcatc
     7261 tgcaccgagc tcgaggaact ggccttcgag cacctcaacc cggtcgcgcg caacgccatc
     7321 ctgcgccggc tcgaggccat gcgcgtcgac cagggcaagg cggtgcagca ggtcagcggc
     7381 gacatcagcc gcatcctcga ggccgagagc atcccggccc gggtgttcgg ccgcgagaag
     7441 catccgttct cgatctggcg caagctgcag cgcaagtcga tcggcttctc gcagatgagc
     7501 gacatctacg ccttccgggt gatcgtcgac agcgaggctg actgctaccg cgcgctgggc
     7561 ctgatccacc gccagtggcc gtgcgtgccg gagcggttca aggacttcat ctccacgccc
     7621 aagcgcaaca actaccgctc gctgcacacc acggtgatcg ggcccaaggg catgcgggtg
     7681 gagatgcaga tccgcaccga ggtcatggac cgggtggccg aggacggcgt ggcggcccac
     7741 tggcgctaca agaacaagtc ctacggcttc gaccgcgagg cgatggaggc cgagggcggc
     7801 cgcgatccgc tggccaacct gcgccagctg gtccagatgc tcgagcacgg cggcgacagc
     7861 gaggacctgg tcgaacacgc caagatggag ctgttcctcg accaggtctt cgtgttcacg
     7921 ccgaagggca agctgatcac cctgccgcgc ggctcgatgc cgctggactt cgcctatggt
     7981 gttcacacca acgtcggcga caccacggtc ggggccaaga tcaatggcga actcaagcct
     8041 ttgcggacgg aacttcagaa cggcgacgtg gtcgagatcg tccgtgggcc gaagccggcc
     8101 gtgctgcccg actggcgctc gctgacggtc accggcaagg cccgctcggc gatccgccgg
     8161 cacatccgcc agaccgagaa ggaagagttc ctgcgcctgg gcgttgcggc catggaccag
     8221 gccttcgccc gggtcggcaa gacccgctcg ggcgtgacct tgcgtccggc gctcgaccgg
     8281 ttcgccgtgg ccggcgagga cgagctgttc gacgccatcg gccgcggccg gatcaccccg
     8341 gccgaggtgc tggagacgat cttccccggc ctcaaggact cggagaaggc cgccgtcacg
     8401 gcccggcgac gcatcaccga cggcaaggag gcccgcgact ttgtcatcgg cgaccggctg
     8461 cgcgcgggga cccttctgcg gttcgccacc tgctgccgcc cggtgccggg cgatcgcatc
     8521 gtcggcatag cggtcgacaa gggggttgag gtgcacgcga tcgactgccc gaccctcgaa
     8581 cagtacgatg accgccagga cctgtggcgc gacctgcact ggacggccga agccgagcgc
     8641 aacacggtct cgcgcacgcg gttgcgggcg accatccgca atgccccggg cgtgctgggc
     8701 gaggcctgca cgatcatcgg ggaggtcggc ggcaacatcg tcaacctgaa catgcaccac
     8761 cgcggcgacg accatttcga cgtcgatttc gacctcgatg tgaaggacgc ccggcacgcg
     8821 accaacatcg tcgccgcgtt gcgcgccaat ccatcgatcg agacggtgga gcgggcgaag
     8881 gggtaggcct cgctcgggga catgcacttc agtgcgtgtc ccctgctatt tggtcagcgc
     8941 tttcaccgcc gccaccgtcg cgctcgtcag cggggcgggc aacgcatcca gcgcgaacca
     9001 gccgcagccg cccagcttgt cgggctccag caccaccggc tcacccagac agtcctcgat
     9061 cagatagacc ggcgcgaccc agtgctgacc atcgcccagg tcgatggtgt cggcgacgca
     9121 cagcagccgg ccgggcgtga tggtcaggcc cagctcctcg gcgatttcgc gggcgcaggt
     9181 ggcgcgggtg tcctcgcccc agtcgacctt gccgcccggc agaccccagt acagcgcctc
     9241 cggctgcttc aggcgttgca ccagcaacag cttgccatcg cgcaggatcg ccgcgccgca
     9301 gccgacacgg ggaacggggg aaggggtggt catgagcccc tctatcccgt ctcccctcgt
     9361 gggagaaggt ggcccgcgaa gcgggtcgga tgaggggttg atgtacgggt cccgtcccga
     9421 ccattccgaa ccgttgcccg cgccctcatc cgtcggccgc gccgacacct tctcccgcaa
     9481 ggggagaagg gttattgagc gactcatgac ccaggacgac gttctcgacg aattccgcgg
     9541 cgccggcgcg ctgcgcgaag gccatttcgt gctctccagc ggcctgcaca gcggggtctt
     9601 cctgcagaag aacctggtgt tcgcccagcc cgaccgctgc gagcggctgt gccgggcgct
     9661 ggcggcgaag atcgtcgccg aggtgggcaa gccggacgtg gccatcagcc cggcggtcgg
     9721 cggcatcatc cccggctacg agaccgcccg gcacctgggc gtccagtcgc tctacgtcga
     9781 gcgcgagggg ggcgccttca agctgcgccg gggcttctcg atcgaacccg gcgcgaaggt
     9841 ggtcatggtc gaggacatcg tcaccacggg tctgtcgtcg cgcgaatgca tcgccgcgat
     9901 ccgcgaggcc ggcggcgaag tcatcgccgc cgcctgcatc gtcgaccgtt caggggggcg
     9961 ggccgacgtc ggcgtgccgc tgatcgcgct ggcgaccctc gacgtgccgg cctacgaggc
    10021 cgacagcctg ccgcccgagc tggccgccat cccggtgcag gatccgggca gccggaggct
    10081 tcgatagttt caccgctcat cccggcgaag gccgggaccc agactttttc tgggacgagc
    10141 ggcgctttac gnnnnnnnnn nnnnnnnnnn nnnctgggtc ccggccttcg ccgggatgag
    10201 cggatttgaa tgcgatgaac cggctacgcc tcggcgtgaa catcgaccat gtggcgacca
    10261 tccggaacgc ccggggggcc ggctatccgg aaccatcgcg ggcggccgag atggcgctcg
    10321 ccgccggggc ggacggcatc accgcccatc tgcgcgagga tcggcgacac atctccgacg
    10381 ccgacatcga cattctggct gatctctgcc tcaagcgtgg caagccgctg aacttcgaga
    10441 tggccgtcac cgacgagatg gtcggcatcg ccctgcgcaa ccgcccgcac gccgcctgtc
    10501 tggtgcccga gcggcgcgag gaggtgacca ccgagggcgg cctcgacgtg gcgggcggcc
    10561 acaatcgcat cgccccggcc gttgaacgcc tgcggtcggc cggctgtcgc gtgtcactgt
    10621 tcatcgaggc cgacccggcg cagatcgaga ccgccgcgaa gatcggcgcc caggtcgtcg
    10681 aactgcacac cggggccttt tgcgacgcct tccgcgacgg cgaccacgac cgcgccgggg
    10741 taattctgaa gcgcctgcgc gacggcgcgg cgctcgccca tgagctcggg ctggaagtcc
    10801 acgccggcca cggcatcgac tacgccaccg tggcgccggt ggccgcgatt cccgagctga
    10861 tggagctgaa catcggccac ttcctgatcg gcgaggcgat cttcatcggg ctcgccccgg
    10921 cgatccagcg gatgcgggtg ctgatggacg ccgccagagc cgagctggcc gcgtgatcgt
    10981 cggcatcggc tccgaccttt ccgacatccg gcgtatcgag aagacgctcg agcggttcgg
    11041 cgaacggttc atcaaccgca ccttcaccgc catcgagcgc gaccggtcgg agcgcaaggt
    11101 caacgcccag ggcggccggg cggccagcta cgccaagcgg ttcgcggcca aggaagcctg
    11161 cgccaaggcg ctcggcaccg gcgtgccccg ccgcggcgtg cactgggccg acatgggcgt
    11221 aatcaacctg cgcagcggca agccgaccat ggccctgacc ggcggggccg ccgagcggct
    11281 ggccgagatc acgccggagg gcatggtggc ggtgattcac ctgtcattga ccgacgatca
    11341 cccctatgcc caggccttcg tgatcatcga ggccgtgccg ccggccggat gaagacgagt
    11401 taacgggcgc gccaaggccc gctagcatag acagtgaacc gggcgggggc gcccgacaga
    11461 accggaaggc catcgccgcc atgaccgacg ccgtggaaag tccagacgac gacagcgctc
    11521 cggcccccgc cgatcccgcg gccaggcgcg ccgaactggt cgagaccctg acgaccgtcg
    11581 cctacgccct gttgatcgcc ctggtgctgc gggtgctgat ctttcagccc ttcaccatcc
    11641 cctcgtcatc gatgcggccc aacctgctga tcggcgacta cgtcgtggtg tccaagttca
    11701 gctacggcta cagccgccac tcgatcccgt tcagcccgcc gctgtttcac ggccggatct
    11761 tcgccggcac gccccggccc ggcgacgtga tcgtcttcaa gacgccggcg acgggtcaca
    11821 aggacctgat caagcggctg atcggcctgc cgggcgacac catccaggtc aaggccggcg
    11881 ccctctacgt caatggccgg gcgctgccgc gggtggctga cggcctggcg accggcgacg
    11941 aggccgcctg tcccaacggc gcgaccccgc tgcgcttcct cgagaccaat ccgatcggcc
    12001 gccgctacgt cacctacgac tgcggcctcg ggccgctcga caacaccccg gttttccgcg
    12061 tgccggccgg ccactatttc ttcatgggtg acaatcgcga caactcgctg gacagccgcg
    12121 tcagcgccct ggccggcggg gtcgactacg ttccggccga aaatctggtc ggcaaggccg
    12181 agacggtgat ggtctcctgg cgggaagggg cctccctgtt caagccgtgg acctgggtgc
    12241 tcaatctgcg ctggaaccgc ttcttccatt cgctgcgata gcggcggccg gatctggccg
    12301 ccacctttac gcctggaagg cgagttaacg ggcgggaagc ttgcccgtgg cctattgcgg
    12361 gactaaccag actcacgggt tcgttcccgc atcaccgaag agagagccgc gcgcgcccat
    12421 gactgacgcc gccgaacccc aggtcgagac cgtcgccgat gccgagccgc tggcgctcga
    12481 acggcgccat gacgacggtc aggaacagac cggcggcggg gcggtcggcg agaccatcga
    12541 gatcgtcaag accgtcgtct acgccctgct gatcgccctt gtcctgcggg tcttcctctt
    12601 ccagccgttc accattccgt cggcctcgat ggagccgaac ctgctcaagg gcgactacat
    12661 catcgtctcg aaatacagct acggttacag caggcattcg atcccgttca gcccgccgct
    12721 gttcaagggc cggatcttct cgcgcgcgcc ggaacggggc gatatcatcg tcttcaagct
    12781 gccgtcggac aatcacaccg actacatcaa gcgcctgatc ggcctgccgg gcgaccggat
    12841 ccaggtgaag ggcggagtgg tctcggtgaa cggcaaggtg ctgacgcggg tctacgacgg
    12901 cccgggcgat ccggggacct gctggaacgg ctcgaccgtg cagcggttca aggagaccaa
    12961 ccccatcggc cgcacctaca tcacctatga ctgcggaccg cgtggcgacg tcgacgacac
    13021 cggcgtctac gtcgtgcccg agggtcacta cttcttcatg ggcgacaatc gcgacaactc
    13081 gctggacagc cgggtcgccc ctgaagccag cggcgtgggc ttcgttcccg ccgagaacct
    13141 ggtcggacac gcgcgcatca tcctgctgtc gtggaacggc gacgcggcgc tgttcaagcc
    13201 gtggacctgg ttcctcgacg cccgtcccag ccggttcttc cacgtcctca aatgaacgcc
    13261 cgcctcgagg ccgtcgcagc cctcgaacag cgcatcggct atgtcttcac cgaccgcgtg
    13321 ctgcttgagc gggccctgac ccacgccagc gtcggcgacg gcgcgcgcaa ggtgacccac
    13381 aacgagcggc tggagttcct cggcgaccgg gtgctcaacc tgctggcggc cgagcgactg
    13441 ctgtcgctcg atccggaggc caaggaaggc gtgctttcgc cgcgcctggc cgccctggtc
    13501 aacggcaagg cctgcgcccg ggtggcccgg cgcatcggcc tgcc
//
LOCUS       DAORNX010000020        13481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3463_length_13481_cov_5.704603, whole genome shotgun sequence.
ACCESSION   DAORNX010000020 DAORNX010000000
VERSION     DAORNX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13481
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_13481_cov_5.704603"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLO65_01705"
     CDS             complement(<1..271)
                     /locus_tag="PLO65_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD26995.1"
                     /translation="MPHLTRWVATAGFAAAVIVGALSPALAQSPPPGYAVPAPLSPTA
                     PIVLTPAQAALLQQTLAESDRHGFEPGEFAPRGPGDAALIDAVVRY"
     gene            591..974
                     /locus_tag="PLO65_01710"
     CDS             591..974
                     /locus_tag="PLO65_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRD26996.1"
                     /translation="MGAMTEIARSRPFMTGFLRGLRHRCPECGEGHLFSRYLKIAPTC
                     DACGHDLGAYKADDGPAYFTILLVGHLIVAPMFFFPFMWEASPWLIVPGALLGLTMVI
                     LLLLPRIKGAFVGALWSIRPDPAHG"
     gene            1144..1299
                     /locus_tag="PLO65_01715"
     CDS             1144..1299
                     /locus_tag="PLO65_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HRD26997.1"
                     /translation="MLWTILIIVLILAVIGALPTWGHSRSWGYLPSGGLGLVLVILLV
                     LFLMGRL"
     gene            1370..2704
                     /locus_tag="PLO65_01720"
     CDS             1370..2704
                     /locus_tag="PLO65_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD26998.1"
                     /translation="MAVTETGVLEDSPGGPPPPGLTPGQRLKAILGGSAGNLVEWYDW
                     STYIFFTVYFASHFFPEGDQTAQLFKAAAVSAVGFLARPLGAWLMGAYADRAGRRAAL
                     SLSVAMMCLGSLIIAFAPTYASVGALAPAILLAARILQGLSVGGEYGASATYLSEVAG
                     ARRRGFWSSFQYVTLIAGSLTAQGMVVLLQQVMPEADLKAWGWRIPFFIGALLAVVVF
                     WIRSGIHESQGFENARAEGENRGRTMLLFVRHPRETLMVLGLTAGGSLAYYVYTSYMQ
                     KFLINTSGFSKATASQIMLVALLCFMLMQPAFGWLSDRIGRKPLLLFAFGGGMIATWP
                     LLNAIAGTTDALTAFGLIMIALVFQSGYSAISGLFKAEMFPAHIRALGVALPYAIANS
                     VFGGSAESVALGFKAAGHESRFYLYVSVILAIGLLTVIFMPDTRKASLIKED"
     gene            2711..3421
                     /locus_tag="PLO65_01725"
     CDS             2711..3421
                     /locus_tag="PLO65_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HRD26999.1"
                     /translation="MPPADLAALAFFALCWLFYEPLLRRLSGGGVLNSDLRVVRAAWM
                     RQMARRESRFMDGQLLGHALNSASFFASSNLLLIAAAAGVLFGGETSFASHSSLELVQ
                     TSSRLLFELQMSVILVVLARGLLSFIWSIRQMNYCLTLFGATPEGEDVDPAIRDAFAE
                     AAGQVLNPALSAFNAGVRGYYFALAAAAWMFGPVAMTLATVGAVSLLVWRQRRSPSAL
                     AIRTLRATLEARETRGLD"
     gene            complement(3557..3898)
                     /locus_tag="PLO65_01730"
     CDS             complement(3557..3898)
                     /locus_tag="PLO65_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRD27000.1"
                     /translation="MTPQAFDAACRALPGVTMDVQWGDDHVFKVGGRMFAVLSPSGGS
                     FSFKASDIAFEVLTAQGPGRPAPYLARAKWVWFADLSTQDDGEVADWLRSAHGLVAAK
                     LTKKARQELGI"
     gene            complement(3895..4548)
                     /gene="msrA"
                     /locus_tag="PLO65_01735"
     CDS             complement(3895..4548)
                     /gene="msrA"
                     /locus_tag="PLO65_01735"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRD27001.1"
                     /translation="MLFAKKTLELPTADTALPGRSRPMPVPPAHFVNGHPLQGPWPEG
                     LEQAVFAMGCFWGVERIFWQIPGVWSTAVGYVAGLTPNPTYEEVCSGRTGHTEAVLVV
                     YDPKVVGYEALLKAFWENHDPTQGMRQGNDMGTQYRSGIYPLNDAQAAAAAASKTAYE
                     QALARVRMGPITTEIADAGPFYYAEDYHQQYLAKNPNGYCGIGGTGVTCPIGVGVGA"
     gene            4758..4847
                     /locus_tag="PLO65_01740"
     tRNA            4758..4847
                     /locus_tag="PLO65_01740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4792..4794,aa:Ser,seq:gga)
     gene            complement(4925..6157)
                     /locus_tag="PLO65_01745"
     CDS             complement(4925..6157)
                     /locus_tag="PLO65_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27002.1"
                     /translation="MTKPAARRFQAPEEGLLASLLLAFLAASGLFYVNIMPALVDGLI
                     SGLGYTSRQAGIVASFNVYGAAAGAFAAVFLVRRFHWKRVVATALGLLIALDLTSILI
                     SDFQVLAAVRFLHGLTGGTVMGMVFGVIARTRTPDKTFGMLLLVQYGIGGLGLLFLPR
                     LVPVFGSGVLFVSLAAFSALAMGTMLFLADYPLAERKAKTAGTGRVRWGPLVLAFAAI
                     FLFQAANMGVSAYVIGLGREAGLGTDYISGALGVANWVGVVGAVLVIVMGLKFGRLWP
                     FLIGMAVTLAGTWAFHFSDQAGLYFVANAVTSVTWSFVIPYLLGMAAAFDETGEMGAL
                     GGFFSKIGLASGPLAGAFILGEGGYAGLVNAGLAGLVLCILAGLAPALMLDRAPIGEA
                     APTLAPDPAAAVGTGPTA"
     gene            complement(6154..>7454)
                     /locus_tag="PLO65_01750"
     CDS             complement(6154..>7454)
                     /locus_tag="PLO65_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27003.1"
                     /translation="SRAAIPFSLQEYNANCTHNGVLTFLYRSNGEISKVAWQIVSETC
                     FYYKFDGWGLVDARYRRGKAPDRAAVTADHRRRVAARLPVRPIAALAADHPDLDVAKF
                     ALKTPADGDPPTVYGVVVDGVNYSGPCQTRRGDYPFCEAVELPGYSTSKSLFAAVALM
                     RLEKLYPGVRDALIVDHVGHCARAGSWDGVTFADALDMATGQYGSLEGSDEEAPLIEA
                     FFTAPNYTARLHYSCYIHPRRVAPGTVFKYHTTDTFVLGAAMSDFLRGKEGESRDLYD
                     DVVLRPLWRPLALGPSLDATLRTYDRYRQPLAGLGLEYHPDDIARLAVWLDRGALIDG
                     RPALDQRMLAAALQRDPADRGLSTGSPTSRYKLGFWGRDIGPLAGCPTPVWVPYMSGY
                     GGISVVLLPHGVQYYYFSDGGGFDWTPAAVEAARIRNPCT"
     assembly_gap    7455..7511
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7512..7879)
                     /locus_tag="PLO65_01755"
     CDS             complement(<7512..7879)
                     /locus_tag="PLO65_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27004.1"
                     /translation="MTRLTTALLAFGLLALAGVAEAAPRRDLAFETLRTGPFAAPVDY
                     AAFAPGATPASNRFEGRLVLDVASGPTGYLERHISLFDRQPATERRKRLPPFDMAFVQ
                     DGADLIPTRHEPPEPADTEWR"
     gene            8001..9128
                     /locus_tag="PLO65_01760"
     CDS             8001..9128
                     /locus_tag="PLO65_01760"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRD27005.1"
                     /translation="MDTAALQVLRLEGGPRERGRQHGETLRPRIAELLERWGDSLERG
                     YGVDRRRYVELFLSRTAYEATTARLAPRVLDEVTGIAEGADVRLSDLLTFQHVNEEFE
                     LAPLFAREAPAGEACSTIAVAPLGDRPSLIAQNLDLAQFLDGFQILLRGPCDQSGGEI
                     LALSVPGMISLNGMNSHGFAVCDNTLVQLRTDPGGLPIYALYRLLLESRSLDEALALV
                     DDLPHAVGLNWVMGDPGGVAMIERSAGQAVRHGPVDPERPAYHTNHPLSCADWGAAFR
                     PGSPGRPRPGRSTYLRLAALHRRLHAPGADVSIDGLKAVLASQDDPDYPVSRGGGANQ
                     EDQQIGFTLACNVFELRRGDPRWHLAAGPPHNHAFRVFSFD"
     gene            complement(9115..10386)
                     /locus_tag="PLO65_01765"
     CDS             complement(9115..10386)
                     /locus_tag="PLO65_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD27006.1"
                     /translation="MSANADLLSRRTAAVPRGVATATPIFAARAENAELWDADGNRYV
                     DFAGGIAVLNTGHRHPKVLAAVRDQLDAYTHTAFQVVAYEPYVALAERLNALAPFDGP
                     AKTILFTTGAEAVENAVKIARAATGRSAVIAFTGGFHGRTMLTMAMTGKVAPYKRLFG
                     PLPAEVFHAPFPIPQYGVSVADSLRALDFLFRADVEPERVAAIVLEPVQGEGGFQPAP
                     PELLRALRDICDRHGILLISDEVQAGFGRTGEAMWGFQRHGLVPDIVTMGKPMGAGHP
                     MAAVVLQPEVIAAFGARVRYFNTFGGNPVSCAVGMAVLDVIENEGLMANALTTGRRLR
                     EGLEALARQYPALGATRGVGLFFGVDIVRDGAPDAAEATRIVNGLRDRRVLISATGPN
                     ANVLKIRPPLVFGPEHADLFLTALGEALSRS"
     gene            complement(10451..11644)
                     /locus_tag="PLO65_01770"
     CDS             complement(10451..11644)
                     /locus_tag="PLO65_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27007.1"
                     /translation="MTDHASTGPVARRGLPTVPEDSLLASVLLSFLATAGLFYVNIMA
                     ALVDGLTSGGLSAKQAGLVASANVYGAAVGALTAVFIIRRLPWRPTAVTVLLCLIAID
                     LVSPLLKAAEVLIAVRFLHGLIGGLLVGVAFAVIARTRTPDRTFGMLLVVQFGLGGLG
                     LMFLPRLVPVFGPGVLFIALAAFSLATLLMTPFLGAYAAAPKVVTAGPRPPTPWLPLV
                     LVFGAIFLFQAANMGLAAYIIGLGKSAGLKLGFITDTLGAANWIGALGSVLVVVMGVR
                     FGRLWPVVAGLALTLAGTWAFHFSEAPWVYLAANIGTAITWAFVIPYLLGMSAALDKA
                     GQMAALAGFFSKMGLASGPAMAAFVAGGGEYDGLINISVLGLALSGLASIAPALALDR
                     QRPPG"
     gene            complement(11641..>13481)
                     /locus_tag="PLO65_01775"
     CDS             complement(11641..>13481)
                     /locus_tag="PLO65_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27008.1"
                     /translation="VVAVHDGADDGVTLTRAAIAPAGADDNSRLSIRVRAMGGKRATC
                     WADAVTVTGERAAVAADAPRVDLTFDSLMTGSALTAPVPMSAFAPPSGAEAAPGRFTG
                     RLGLDLSGVMLAMNVLHDDTGDSPAEAAARATLPPLDVDFVQDGGDLLPVRRGVVVGD
                     HPAWDWVVEPGRVWREAGDRGWLRAAVPFALQERNANCLHNGMLTFLFKPDGSVSRLA
                     LEIASETCAYLKFDAWATVPARLAPAAVPEADAIVAAWREEIAARLPVRPLAELARLR
                     PDVNLAALALGATTDGDPPSALGLVVDGVHYAGPCRTRHGDYPVCDVLDLPSYSTAKS
                     IVGGVGLMRLEALHPGSASALIRDHVPACVRGNDWAGVTLADALDMATGVYGSAAFEA
                     DENAPAGRVFFDAEDHAAKAAYACGQFRRRAAPGTTFVYQTSATYLLGAAMSDRLDRA
                     GEGDLYDDLVAPLWRALRLSPTIMGTRRTYDAARQPFTGWGLTYHRDDILRIAGWLEG
                     GAVIDGRPALDSALLTAALQKDPAPSGLQAGGPNYRYKGGFWARNIGGPLGCPAPVWT
                     PFMSGFGGISVVLLPGGVTYYYFGDSGVFDWAPAAVEAARIKDMCP"
BASE COUNT         2050 a         4740 c         4705 g         1929 t
ORIGIN      
        1 cgtagcgcac caccgcgtcg atcagggcgg cgtcgccggg accgcgcggc gcgaactcgc
       61 ccggttcgaa gccatggcga tcggactccg ccagcgtctg ttgcagcagg gccgcctggg
      121 ccggcgtcag gacgatgggc gcggtcggcg acagcggcgc gggaacggcg tacccgggcg
      181 gcggcgactg agccaacgcc ggcgacaggg cgcccacaat cacggccgcg gcgaaaccag
      241 ccgtggcgac ccaacgcgtc agatgcggca tcgaacccgt acgccctctc atgcccagcc
      301 cggcgaccct agcagagcat tgcgcggttc gcgcaatgtt gtcgcccctg acgcgaaaag
      361 agccgggggc tgtggccgcc ctgcccctcc cgccggcgcg cctggatgtc ggttcgttca
      421 ggtgtcgcaa attcggcgcc ctccacagtt ctgctcgccc ccgccacggg cccgcctgac
      481 ggcgcgcctg cccgagggga caacgccggc ggccggtcgc ggttcagggg ccgaatgtcg
      541 cagcgtttcg ccccctcgcc agtctcgcct tttgcgccgt catggcccat atgggcgcca
      601 tgaccgaaat cgcccgatcg cgcccgttca tgacgggctt cctgcgcggc ctgcgccatc
      661 gctgccccga atgcggcgag ggccatctgt tcagccgcta tctcaagatc gccccgacct
      721 gcgacgcctg cggccatgac ctcggcgcct acaaggccga cgatggtccc gcctatttca
      781 ccatcctgct ggtcggccac ctgatcgtcg cgccgatgtt cttctttccc ttcatgtggg
      841 aagcctcgcc ctggctgatc gtgcccggcg cgctgctggg cctgaccatg gtgatcctgc
      901 tgctgttgcc gcgcatcaag ggcgccttcg tcggagccct gtggtccatc cggccggatc
      961 cggcccacgg ctgaggacgc cgcccggtcg gccatgacga ctccagtctg accgtccctc
     1021 tcgcgcgacc gcggccggac ggcgctggcc cgcgccgctg cgcctgaacg ccctccggaa
     1081 ccccgggcct cttccgggaa ttgatcccgc gaagccgggg ccaatctgtt caggaggcag
     1141 cgcatgctgt ggaccatcct cattatcgtt ctgatcctcg ccgtcatcgg cgcgctgccg
     1201 acctggggcc attcccggtc gtggggctac ctgccgagcg gcgggctggg cctagtgctg
     1261 gtcatcctgc tggtgctgtt cctgatgggc cggctgtagg ccagacgccc ttcccctgcc
     1321 gactcggcgc gcctaacctc ccggacacga acgtgttcgg ggggatggca tggcggtcac
     1381 ggaaactggg gtcctggaag acagccccgg cgggccgccc ccgcccggcc tgaccccggg
     1441 ccagcgcctg aaggccattc tcggcggctc ggccggcaac ctggtcgagt ggtacgactg
     1501 gtcgacctac atcttcttca ccgtctattt cgccagccac ttctttcccg agggcgacca
     1561 gacggcgcag ctgttcaagg ccgcggcggt gtcggccgtc ggcttcctcg cccggccgct
     1621 tggagcctgg ctgatgggcg cctacgccga ccgggccggc cgccgcgcgg ccctcagcct
     1681 gtcggtggcg atgatgtgcc tgggcagcct gatcatcgcc ttcgccccga cctatgccag
     1741 cgtgggcgcc ctggccccgg ccatcctgct ggccgcccgc atcctgcaag ggctgtcggt
     1801 cgggggcgaa tacggcgcca gcgccaccta cctctccgag gtcgccgggg ccaggcggcg
     1861 cggcttctgg tccagcttcc agtacgtgac cctgatcgcc ggctccctga ccgcccaggg
     1921 catggtcgtg ctgctgcagc aggtcatgcc cgaggcggac ctcaaggcct ggggctggcg
     1981 catcccgttc ttcatcgggg cgctgctggc ggtggtggtg ttctggatcc gaagcggcat
     2041 ccacgagagc cagggcttcg agaacgccag ggcggagggc gagaaccgtg gccgcaccat
     2101 gctgctgttc gtcagacacc cgcgcgagac cctgatggtc ctcggcctga ccgccggcgg
     2161 ttcgctcgcc tactacgtct acaccagcta catgcagaag ttcctgatca acacctccgg
     2221 cttctccaag gccacggcca gccagatcat gctggtcgcc ctgctgtgct tcatgctgat
     2281 gcagccggcg ttcggctggc tgtcggaccg catcggccgc aagccgctgc tgctgttcgc
     2341 cttcggcggc ggaatgatcg ccacctggcc gctgctcaac gccatcgccg gaaccaccga
     2401 cgccctgacg gccttcgggc tgatcatgat cgccctggtg ttccagtccg gctacagcgc
     2461 catttcgggg ctgttcaagg ccgagatgtt cccggcccat atccgcgccc ttggcgtggc
     2521 cctgccctac gccatcgcca actcggtgtt cggcggctcg gcggaatcgg tcgctctggg
     2581 cttcaaggcc gccgggcatg aaagccgctt ctacctctat gtcagcgtga tcctcgccat
     2641 cggcctgctg accgtgatct tcatgcccga cacccgcaag gccagcctga tcaaggaaga
     2701 ctgatcccgc atgccgcccg ccgacctcgc cgccctggcc ttcttcgccc tctgctggct
     2761 gttctacgag ccgctgctgc ggcggctgtc cggcggcggg gtgctcaaca gcgacctgag
     2821 ggtcgtgcgc gcggcctgga tgcgccagat ggcccgccgc gagagccggt tcatggacgg
     2881 ccagctgctc ggccatgcgc tgaactcggc ctccttcttc gcctcgtcca acctgttgct
     2941 gatcgccgcg gccgccgggg tgctgttcgg cggcgagacg tcgttcgcca gccactccag
     3001 cctcgaactg gtgcagacct ccagccggct gctgttcgaa ctgcagatgt cggtgatcct
     3061 ggtggtgctg gcccggggcc tgctgtcctt catctggtcg atccgccaga tgaactactg
     3121 cctgaccctg ttcggggcga ccccggaggg cgaggatgtc gatccggcga tccgcgacgc
     3181 cttcgccgag gcggccggcc aggtgctcaa tccggccctg tcggcgttca acgccggcgt
     3241 gcgcggctac tatttcgccc tggccgcggc cgcctggatg ttcggccccg tggccatgac
     3301 cctggccacc gtcggcgcgg tcagcctgct ggtgtggcgc cagcgccggt cgccgtcggc
     3361 cctggcgatc cgcacattgc gggcgacgct ggaagcgcgg gagacgcggg gactggacta
     3421 gggccaagga acagtcacga cccggggccg ttttttcacc gatggacggc tgccgtgcgc
     3481 tgaaatgtcg attggccgcg gcgctgtcct gacccccggc cgcggggcca ggacaggcaa
     3541 gcccgccccc gcattgctag atcccgagtt cctgccgggc cttcttcgtc agcttcgccg
     3601 cgaccaggcc gtgcgccgat cgcagccagt cggcgacctc gccgtcgtcc tgggtcgaca
     3661 ggtcggcgaa ccagacccac ttggcgcgcg ccaggtaggg cgccggcctg cccgggccct
     3721 gcgcggtcag cacctcgaag gcgatgtcgc tggccttgaa gctgaagctg ccgcccgacg
     3781 gcgacagcac ggcgaacatc ctgccgccga ccttgaacac atggtcgtcg ccccactgca
     3841 cgtccatcgt caccccgggc agggcgcgac aggccgcgtc gaaggcctgt ggcgtcacgc
     3901 gccgacgccg acgccgatcg gacaggtcac gcccgtgccg ccgatgccgc aatagccgtt
     3961 cgggttcttg gccagatact gctggtggta gtcctcggcg tagtagaacg gccccgcgtc
     4021 ggcgatctcc gtggtgatcg gccccatgcg gaccctggcc agcgcctgct cataggcggt
     4081 cttcgaggcc gccgcggcgg cggcctgggc gtcgttcagc ggatagatgc ccgagcggta
     4141 ctgggtgccc atgtcgttgc cctggcgcat gccctgggtc gggtcgtggt tctcccagaa
     4201 ggccttcagc agggcctcgt agccgaccac cttcgggtcg tagaccacca gcacggcctc
     4261 ggtgtggccg gtgcggccgc tgcagacctc ctcgtaggtg gggttgggcg tcaggcccgc
     4321 gacatagccg acggcggtgc tccagacgcc ggggatctgc cagaagattc gctcgacccc
     4381 ccagaagcag cccatggcga acaccgcctg ctccaggccc tcgggccagg gcccctgcag
     4441 cggatggccg ttgacgaaat gggcgggcgg gaccggcatc ggccggctcc ggcccggcaa
     4501 ggcggtgtcg gcggtgggaa gttcaagggt ctttttggcg aacagcatgg cgtcctcgac
     4561 ggtcggttcg ggcccaacat aggcgcgccg cgtcgggatt tcaccgtccg actggtgatc
     4621 gtcgccatgc cgcgccgtca tcgccccgac gccggccgtg acgggccgcc agtgcgcgct
     4681 tgcccccggg gggcccatga gtatattgcg cgccttcctg cgccgggggc cttgaccagc
     4741 tcctgaccgg tggtgctggt gaggtggccg agtggtcgat ggcgcacgct tggaaagcgt
     4801 gtgtaggtta acgcctaccg tgggttcgaa tcccactctc accgccaccg gccggaagtc
     4861 tctcgcgccg atctctcgac cttccgtccg atcgcgccgt cagcgcctga cgcgcggccc
     4921 ctgctcaggc cgttggtccg gtcccgacgg ccgcggcggg gtcgggcgcg agggtcggcg
     4981 ccgcctcgcc gatcggcgcg cgatcgagca tcagggccgg cgccaggccc gcgaggatgc
     5041 atagaaccag ccccgccaga ccggcgttga ccagcccggc atagccgccc tcgccgagga
     5101 tgaaggcgcc ggccagcggc ccggaggcca ggccgatctt ggagaagaac ccgcccaggg
     5161 cgcccatttc gccggtctcg tcgaaggcgg ccgccatccc cagcagatag ggaatgacga
     5221 acgaccaggt caccgaggtc acggcgttgg cgacgaagta gaggccggcc tggtcgctga
     5281 aatggaaggc ccaggttccc gccagggtta ccgccatgcc gatcaggaac ggccacagcc
     5341 gtccgaactt caggcccatg acgatgacca gcaccgcgcc gacgacgccg acccagttgg
     5401 cgacgcctag ggcgccgctg atgtagtcgg tgcccagtcc ggcctcgcgg cccaggccga
     5461 tgacataggc cgagacgccc atgttggcgg cctggaacaa gaagatggcg gcgaaggcca
     5521 gcaccagggg cccccacctg acgcgaccgg tcccggctgt cttcgccttg cgctctgcca
     5581 gcggatagtc ggccaggaag agcatggtcc ccatggccag ggcgctgaag gcggcgagcg
     5641 agacgaacag cacgcccgag ccgaacaccg gcaccagccg gggcaggaag agcaggccca
     5701 gcccgccgat gccgtactgc accagcagca gcatgccgaa ggtcttgtcc ggcgtgcggg
     5761 tgcgggcgat gacgccgaac accatgccca tcaccgtccc cccggtgaga ccgtggagga
     5821 aacggaccgc ggcaaggacc tggaagtcgc tgatcaggat cgacgtgaga tcgagcgcga
     5881 tcagcaggcc gagcgccgtc gccaccaccc gcttccagtg aaaccgccgg acgaggaaga
     5941 cggccgcgaa cgcgccggcc gcggcgccgt agacgttgaa cgaggcgacg atgccggcct
     6001 gcctggacgt gtagcccagc ccgctgatca ggccatcgac caacgccggc atgatgttga
     6061 cgtagaacag tccggacgct gcgagaaagg ccagcagcag gctggccagc aggccctctt
     6121 ccggcgcctg gaagcggcgg gcggcaggtt tggtcatgtg cagggattcc tgatcctggc
     6181 ggcctcgacc gccgccggcg tccagtcgaa accgccgccg tcgctgaagt aatagtattg
     6241 aacaccgtgc ggcagcagca ccacggagat gccgccatag ccggacatgt acggaaccca
     6301 gaccggcgtc gggcagccgg ccaggggccc gatgtcccgg ccccagaagc ccagcttgta
     6361 gcgactggtc ggcgaccccg tggaaaggcc ccggtcggcc gggtcgcgct gcagcgccgc
     6421 ggccagcatg cgctggtcga gcgccggccg gccatcgatc agggccccgc ggtcgagcca
     6481 gaccgccagg cgcgcgatgt cgtccgggtg atattcgagc cccagtccgg ccaggggctg
     6541 gcggtagcgg tcataggtcc tgagcgtcgc atcgagcgac ggtccaagag ccagcgggcg
     6601 ccaaagcggc cggagaacca cgtcgtcgta gagatcgcgg ctctcgccct ccttgccgcg
     6661 caggaagtcg ctcatcgccg caccgagcac gaaggtgtcg gtcgtgtggt acttgaacac
     6721 ggtccccggc gcgacgcggc gggggtggat gtagcagctg tagtgcagtc gcgcggtata
     6781 gttgggcgcg gtgaaaaagg cttcgatcag gggagcctcc tcgtcggagc cttccaggga
     6841 gccgtactgg ccggtggcca tgtcgagcgc atcggcgaag gtcaccccgt cccagctccc
     6901 ggcgcgggcg cagtggccga catggtcgac gatcagcgcg tcgcgcacgc ccggatacag
     6961 cttttccagc cgcatcaacg ccacggccgc gaacaacgac ttgctggtcg agtagccggg
     7021 cagctcgacc gcctcgcaga acggatagtc gcccctgcgg gtctggcagg ggccggaata
     7081 gttcacgccg tcgacaacca cgccgtagac cgtgggcgga tcgccgtcgg cgggggtctt
     7141 cagggcgaac ttcgcgacat cgaggtcggg gtgatcggcc gccagggccg cgatcggcct
     7201 gaccggcagc cgggccgcca cgcggcgacg atggtcggcg gtcaccgccg cgcggtccgg
     7261 cgccttgccc cgccgatagc gggcgtcgac caggccccag ccgtcgaact tgtagtagaa
     7321 acaggtctcg ctgacgatct gccaggcgac cttcgatatc tcgccgttcg accggtagag
     7381 gaaggtcagc acgccgttgt gggtgcagtt ggcgttgtat tcctgcaggc tgaacgggat
     7441 ggccgcccgg ctccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn ncgccactcg gtatcggccg gctccggcgg ttcatgccgc gtcgggatca
     7561 ggtcggcgcc atcctggacg aaggccatgt cgaagggcgg caggcgcttg cgccgctcgg
     7621 tcgccggctg gcgatcgaac aggctgatgt gccgctcgag gtagccggtc ggtccggacg
     7681 ccacgtccag caccagccgg ccctcgaacc ggttggaggc cggcgtcgcc cccggcgcga
     7741 aggcggcgta gtcgaccggc gccgcgaacg gccctgtccg caaggtctcg aacgcgagat
     7801 cgcggcgggg cgcggcctcg gcgacgccag ccagcgcgag cagaccgaac gccagcagag
     7861 cagtcgtcaa gcgcgtcatg tcgcgggggc cccatccgcc atggcctgaa gacacgcagg
     7921 ccgcagcgtt ccaccatggc ggctcgtcgc gggcggggtg tcctgaacga gagcctcgtc
     7981 ggcagggaga gggcccggaa atggatacgg cggcgttgca ggtcttgcga ctcgagggcg
     8041 gccctcgcga acgcggtcgc cagcacggcg aaacactgag gccccggatc gcggaattgc
     8101 tggaacgctg gggcgacagc ctcgagcgcg gctatggcgt cgaccgccgc cggtatgtcg
     8161 aactgttcct ctcgcgcact gcctacgagg cgaccacggc gcgactggcg ccccgggtgc
     8221 tcgacgaggt gacgggcatc gccgagggcg ccgacgtccg cctaagcgat ctgctgacct
     8281 tccagcacgt caacgaggag ttcgagctcg cgccgctgtt cgcgcgcgag gccccggccg
     8341 gcgaggcctg cagcacgatc gccgtggcgc cgctgggcga ccggcccagc ctgatcgccc
     8401 agaatctcga cctggcccag ttcctcgacg gtttccagat tctgctgcgc ggaccgtgcg
     8461 accagtcggg cggcgaaatc ctggccctgt cggtcccagg catgatctcg ctgaatggca
     8521 tgaacagcca cggcttcgcg gtctgcgaca acaccctggt gcagctgcgc accgatccgg
     8581 gcggcctgcc aatctatgcg ctctaccgcc tgctgctcga gagccggtcc ctggacgagg
     8641 cgctggccct ggttgatgac ctgccccatg ccgtggggct gaactgggtg atgggcgacc
     8701 ccggcggggt ggcgatgatc gagcggtcag cgggccaggc cgtgcgccac ggcccggtcg
     8761 atccggaacg gccagcctat cacaccaacc atcccctgag ctgcgccgat tggggcgcgg
     8821 cgttccggcc cggatcaccc gggcggcccc ggccaggccg cagcacctat ctgcggctgg
     8881 cggccctgca ccggcgcctg catgcgccgg gcgccgacgt cagcatcgac gggctcaagg
     8941 cggtcctcgc ctcgcaggac gacccagact atccggtgtc gcggggcggc ggggccaacc
     9001 aggaagacca gcagatcggc ttcaccctgg cctgcaatgt tttcgagctg cgacgcggcg
     9061 acccgcgctg gcacctggcc gccggcccgc cgcataacca cgccttccgg gtgttcagct
     9121 tcgactgagc gcctcgccca gggcggtcag gaacaggtcg gcgtgctccg gcccgaacac
     9181 cagcggcggg cggatcttca gcacattggc gttcgggccg gtggcgctga tcagcacccg
     9241 gcggtcgcgc agcccgttga cgatgcgcgt cgcctccgcg gcgtccggcg cgccgtcgcg
     9301 aacgatatcg acgccgaaga acagacccac gccccgcgtc gcgccaagcg ccggatactg
     9361 tctcgccaga gcctcgaggc cctcgcggag gcgccggccc gtggtcaggg cgttggccat
     9421 caggccctcg ttctcaatga cgtcgagcac cgccatgccg acggcgcagg acacggggtt
     9481 gccgccgaag gtgttgaagt agcgcacccg ggcgccgaag gcggcaatca cttcgggctg
     9541 cagcacgacc gccgccatcg ggtggccggc gcccatcggc ttgcccatgg tcacgatgtc
     9601 cggaaccagg ccgtgccgct ggaagcccca catggcctcg ccggtgcggc cgaacccggc
     9661 ctggacctcg tcggagatca gcaggatgcc gtgacggtcg cagatgtcgc gcagcgcgcg
     9721 cagcagctcg ggcggcgcgg gctggaagcc gccctcccct tgcacgggtt cgagcacgat
     9781 ggccgccacc cgttccggct ccacgtcggc gcggaacagg aagtcgagcg cgcgcaggct
     9841 gtcggcgacg ctgacgccat actgtggaat ggggaatggc gcatgaaaga cctcggccgg
     9901 cagcgggccg aacaggcgct tgtagggtgc gaccttgccg gtcatggcca tggtcagcat
     9961 ggtccggccg tggaagccgc cggtgaaggc gatgaccgcg gatcggcccg tcgccgcgcg
    10021 agcgatcttc acggcgttct cgaccgcctc ggcgccggtg gtgaacagga tggtcttggc
    10081 cggtccgtcg aacggcgcca gcgcgttgag ccgctcggcg agcgcgacat agggctcata
    10141 ggccaccacc tggaaggcgg tgtgggtgta ggcgtcgagc tggtcgcgaa ccgcggccag
    10201 gaccttcgga tgccggtggc cggtgttgag cacggcgatg ccgccggcga aatcgacgta
    10261 gcgattgccg tcggcgtccc acagctcggc gttctcggcg cgggcggcga agatgggggt
    10321 ggcggtggcg acgccgcgag gcacggcggc cgtgcggcga gacaacaggt cagcgttggc
    10381 ggacatggga atgtactcct gaaggcagat gcggaatgtc ggccgccggg gcggccggag
    10441 gggatggggg tcagccgggt gggcgctgtc ggtcgagcgc cagggccggg gcgatcgacg
    10501 ccagcccgct gagcgccagg ccgaggaccg agatgttgat caggccgtca tattcgccgc
    10561 cgccggcgac gaaggcggcc atggcggggc ccgacgccag ccccatcttc gagaagaagc
    10621 cggcgagcgc ggccatctgc ccggccttgt ccagggcggc gctcatgccg agcagatagg
    10681 ggatgacgaa ggcccaggtg atcgccgtgc cgatgttggc ggccaggtag acccacggcg
    10741 cctcgctgaa atggaaggcc caggtgccgg cgagggtcag ggcgaggccc gccacgaccg
    10801 gccacagccg gccgaacctg acgcccatca cgaccacgag cacagagccg agcgcgccga
    10861 tccagttggc cgcgcccagg gtgtcggtaa tgaagcccag cttcaggccc gcgctcttgc
    10921 ccaggccgat gatataggcg gccagcccca tattggcggc ctggaacagg aagatcgcgc
    10981 cgaacaccag gaccagcggc agccaggggg tcggcggccg gggccccgcc gtcacgacct
    11041 tgggcgcggc ggcgtaggcg cccaggaagg gcgtcatgag cagggtcgcc aggctgaagg
    11101 ccgccagggc gatgaacagc acgccaggtc cgaacaccgg caccagccgc ggaaggaaca
    11161 tcagtcccag cccgccgagg ccgaactgga ccaccagcag catgccgaag gtgcggtccg
    11221 gcgtccgggt gcgggcgatc accgcgaagg ccacaccgac cagcagcccg ccgatcaggc
    11281 catgcaggaa gcgcaccgcg atcaggacct ccgccgcctt caaaagcggc gagaccagat
    11341 cgatggcgat caggcagagc aacaccgtca cggccgtcgg ccgccagggc aatctgcgga
    11401 tgatgaacac cgccgtcagc gcgccgacgg cggcgccata gacgttggcc gaggccacca
    11461 gtcccgcctg cttggcggag agcccgccgc tggtcaggcc gtcgacgagc gcggccatga
    11521 tgttcacgta gaacaggccg gcggtggcga gaaaggacag caggacgctg gccagcaggc
    11581 tgtcctcggg aactgtcggc agacctcggc gcgcgaccgg cccagtggaa gcgtggtcgg
    11641 tcatgggcac atatccttga tgcgggccgc ctcgaccgcg gccggagccc agtcgaacac
    11701 gccgctgtcg ccgaagtagt agtaggtcac gccgcccggc agcagcacga cggaaatgcc
    11761 gccgaagccc gacatgaacg gcgtccagac gggcgccgga caccccagcg gcccgccgat
    11821 gttccgcgcc cagaagccgc ccttgtagcg atagttgggt ccgccagcct gcagcccgga
    11881 gggggccgga tccttctgca gcgccgcggt caacagcgcc gagtcgagcg ccggccgccc
    11941 gtcgatcacc gcgccgccct cgagccagcc ggcaatgcgc aagatgtcgt cgcgatgata
    12001 ggtcagaccc cagccggtga acggctggcg ggccgcgtca taggtccggc gcgtgcccat
    12061 gatggtcggg ctgagccgca gcgcgcgcca cagtggtgcg accagatcgt cgtagagatc
    12121 gccctctccg gcccggtcga gccgatcgga catcgccgcc cccagcagat aggtggcgct
    12181 cgtctggtag acgaaggtcg tcccgggcgc ggcccggcgg cggaattgtc cgcaggcgta
    12241 ggccgccttg gcggcatggt cttcggcgtc gaagaacacc cggccggccg gcgcgttctc
    12301 atcggcctcg aaggccgctg aaccatagac gccggtcgcc atgtcgaggg cgtcggcgag
    12361 cgtcaccccg gcccagtcgt tcccccggac gcacgccggc acgtgatcgc ggatcagggc
    12421 cgacgcgctg ccgggatgca gggcctcgag ccgcatcagc cccacgccgc ccacgatcga
    12481 cttggccgtc gagtaggacg gcaggtcgag cacgtcgcag acgggatagt cgccgtggcg
    12541 ggtccggcag ggaccggcgt aatgaacgcc gtcgacgacc agtcccaagg ccgagggcgg
    12601 gtcgccgtcg gtggtcgcgc ccagcgccag ggcggcgagg ttcacatccg gacgcagccg
    12661 cgcgagttcg gcaaggggac ggaccggcag ccgcgccgcg atctcctccc gccaggcggc
    12721 gacgatcgcg tcggcttccg gaacggccgc cggcgcaagg cgcgccggga ccgtggccca
    12781 ggcgtcgaac ttcagatagg cgcaggtctc cgaggcgatc tccagcgcca ggcgggacac
    12841 cgagccatcg ggcttgaaga ggaaggtcag catgccgttg tgcaggcagt tggcgttgcg
    12901 ctcctggagg gcgaacggga ccgccgcccg cagccagccg cgatcccccg cttcgcgcca
    12961 gacccgcccc ggctccacga cccaatccca ggccggatga tcaccgacca cgaccccgcg
    13021 ccggaccggc agcaggtcgc cgccatcctg cacgaagtcg acgtccaacg gcggcagcgt
    13081 cgcgcgcgcg gccgcttccg ccggactgtc gccggtgtcg tcgtgaagga cgttcatggc
    13141 cagcatcacg ccggagagat ccaggccaag gcgtcccgtg aaccgtccag gcgcggcttc
    13201 ggcgccggac ggcggcgcga aggcggacat gggaaccggc gcggtcaacg ccgaccccgt
    13261 catcaggctg tcgaaggtca ggtcgaccct cggcgcatcc gccgcgacgg cggcgcgctc
    13321 gccggtcacc gtcaccgcgt ccgcccaaca ggtcgcccgc ttgccgccca tcgcgcgaac
    13381 ccggattgac agccgagagt tgtcatccgc gccggccggc gcgatggcgg cgcgggtcag
    13441 ggtcacgccg tcgtcggcgc cgtcgtggac cgcgacgact t
//
LOCUS       DAORNX010000021        13454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3469_length_13454_cov_7.740876, whole genome shotgun sequence.
ACCESSION   DAORNX010000021 DAORNX010000000
VERSION     DAORNX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_13454_cov_7.740876"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLO65_01780"
     CDS             <1..313
                     /locus_tag="PLO65_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRD27009.1"
                     /translation="DDAAHEILRTLPRLDDGALMVLADSFRGPEGQARFDLLFDRLSA
                     QIRTMAVGQAMAGVGEGLDRWALAWDQLQRLPDQVEGLNLDRADAFWTALRDLRAAAA
                     A"
     gene            321..1100
                     /locus_tag="PLO65_01785"
     CDS             321..1100
                     /locus_tag="PLO65_01785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRD27010.1"
                     /translation="MLIDSHVNLHAPQFAEDRGAVIDRARAAGIALMVNISDRVSTFE
                     TTYAVAEANPDIWATVGTHPHEAKENPHLTARDIIDLAARPKVVGIGETGLDFHYDLS
                     PREVQAAVFRQHVIAARETGLPLVVHTREADDVMARILEEEYAAGPFRLLMHCYTSGA
                     DLARRAAALGAWFSVSGIATFKAAQDVRDVIADMPAERIIVETDCPYLAPVPMRGRRN
                     EPAFLPHVLAKLAEIRGWTLAEAEQHTEDAFFALFDRIPRP"
     gene            1097..1900
                     /locus_tag="PLO65_01790"
     CDS             1097..1900
                     /locus_tag="PLO65_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD27011.1"
                     /translation="MSGVLEVTILGSGSSGGVPRADGNWGDCDPAEPRNRRSRCGLLL
                     RRKGEGAETTVLVDTSPDLREQLIAAGVQRVDAILFTHDHADQVHGVDDVRAFYLRQR
                     ARIPAWMDPRTRSVLERRFDYIFKDMGGYPAILEARDLPAMGGSFRIDGPSGPIPVTT
                     FDQEHGHIRCVGYRFGDVAYSPDVNGLPESAFEALAGVDTWIVDALRRTPHPTHAHLR
                     QTLDWIARVRPRRAILTNLHIDMDYRQLCEELPRGIEPAYDGLSLEIGL"
     gene            2071..2580
                     /locus_tag="PLO65_01795"
     CDS             2071..2580
                     /locus_tag="PLO65_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27012.1"
                     /translation="MRLATFLSTTLIAIAAAFAAQAAPLGPVAVVHVTIGPQLQDKAD
                     EYGQQELDYLAADLKDSVERALERSGGLKPSGGTLDLVIEDALPNRPTMRQMNIKPGL
                     SYESFGIGGAKVGGVLTTPDGRQVSVGYGWYETDIRWSEGASTWMDALNTFDRFARRL
                     VTGEAVLDH"
     gene            2734..4167
                     /locus_tag="PLO65_01800"
     CDS             2734..4167
                     /locus_tag="PLO65_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27013.1"
                     /translation="MAGGRRSTLTLAAFAAPCLPLAAFGLPLTISLPHYYSETLGLQV
                     SAVGLAFLLVRLVDIGFDPVFGTILDRTRWSFGRFKGWMAIATPVLMLAIYMLFMARP
                     GVTTGYLWFWLIVVYFGYSIAVLSHTSWAAKLTTDYHERSRIYAFWQTGNVIGMIMIL
                     AMPLVLNLASRGIGQEGILHGQGWFIVLLLPAMVALTLWRVPEPRPVESTVRDSGGAL
                     VSQSANLGDYFRLLRRPTVIRILAADLLTGLAPGVAGTLFFFYFMRVKQFSFLESSAL
                     LLIYFVAAIFGAMIWTRLAKRMGKSKALIAACVTYAAVQFAVVTLPVGNFWLATPFLI
                     AAGIPYSAGALLLRSMMADYGDEERLSSGVDRTGLLFAILTGTVKIGSALAVTSLILL
                     GALGFDAAHAANSTPLSMTGLQALYAFAPGIVGLLAAAAMIGYPLTEAKHAEILRQLG
                     ERDAVFPPVVPEDVAGTEPNVANPAVT"
     gene            4142..4951
                     /gene="mazG"
                     /locus_tag="PLO65_01805"
     CDS             4142..4951
                     /gene="mazG"
                     /locus_tag="PLO65_01805"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRD27014.1"
                     /translation="MSQTPQSPEGDHPVFRLLEIMDRLRDPAGGCAWDLEQTFATIAP
                     YTVEEAYEVADAIERNDLKDLREELGDLLFQVVFHARMANEQGAFDFADVAAAISDKM
                     IRRHPHVFGDASRTAGEQTAAWEVLKQQERAGKARHGILDDVPVGLPALTRAVKLSKR
                     AARVGFVWPSVKEVLDKLDEEVAELKAEVAGGDIDRLRGELGDVLFVIANLARTLDIE
                     PEDALRSTNAKFVRRFGYIEEALAARGKTPEMSDLAEMDALWDEAKAAERG"
     gene            complement(5019..6335)
                     /gene="hflX"
                     /locus_tag="PLO65_01810"
     CDS             complement(5019..6335)
                     /gene="hflX"
                     /locus_tag="PLO65_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRD27015.1"
                     /translation="MTQLIDHEDPVEKALVLHPVRPSQANSRDPQARLEEATGLALAL
                     DLDVVESRIVPLRERTPATLFGKGKVEELRLLCEVEEIDVVVVDDALSPVQQRNLEKA
                     WQVKVVDRSGVILEIFGRRARTREGVLQVELARLEYERSRLVRTWTHLERQRGGTGST
                     GGPGETQIELDRRQIADRIIKMKKELVEVRRTRALHRTQRKKAPYPTIALVGYTNAGK
                     STLFNRITAGGVLAKDMLFATLDPTLRNVKLPGGRPAILSDTVGFISDLPHELIESFR
                     ATLEEVQEADVILHVRDIAGPDTQAQKRDVETVLTELGVTPESGRKLIEVWNKADLLS
                     PEARDEAEGAARRSGALLVSSITGEGCEALLNRLASLVDETPEIDVQLSSNAGEALAW
                     LYRHGRVVGRHEGGEGDLQLRVRLDGQALGRFERLFPDAKVSEAEG"
     gene            complement(6332..6574)
                     /gene="hfq"
                     /locus_tag="PLO65_01815"
     CDS             complement(6332..6574)
                     /gene="hfq"
                     /locus_tag="PLO65_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRD27016.1"
                     /translation="MTDKKQNLQDTFLNSVRKSKTPLTIFLVNGVKLQGVVSWFDNFC
                     VLLRRDGQSQLVYKHAISTIMPAQPVQLYEPSEQDD"
     gene            complement(6682..7545)
                     /locus_tag="PLO65_01820"
     CDS             complement(6682..7545)
                     /locus_tag="PLO65_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRD27017.1"
                     /translation="MSRWAYVNGRFVRHGEAAVHIEDRGYQLADGIYEVWAVMGGRLV
                     DSDGHFERLDRSLGEMGIRPPMSRKALELVLREALRRNRVRHGLLYLQVTRGVARRDH
                     AFPIPAPEPAIVITARSIDPIAAEVRAARGVSVITTPENRWGRCDIKTIGLLPNAMAK
                     TAARDRGAAEAWFVDADGLVTEGASSNAWIVDAQGVLRTRDTTANILRGVTRKAVLGL
                     LGEANIRLDERAFTPEEARNAREAFITGAGALILPVVAIDGAPIGTGKPGPVAQRLRA
                     LYIQNAQASNS"
     gene            complement(7545..8936)
                     /locus_tag="PLO65_01825"
     CDS             complement(7545..8936)
                     /locus_tag="PLO65_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRD27018.1"
                     /translation="MAADVLVVDDEADIRELVSGILTDEGYAVRTAADSEQALLAIRA
                     RKPTLVVLDIWMQGGGMDGLELLDLVKALDADLPVIMISGHGNIETAVSAIKRGAYEF
                     LEKPFKSDRLLLMVERAIESASLRRENRRLRTQALTPDGLIGRSLAAQQLRQMIAKVA
                     PANSRVLIAGPAGSGKELVARMIHAASPRAKGEFVAISAAGMAPERMDLELFGEEGEG
                     SKPGKIGVFERAHGGTLYLDEVADMPRETQSRILRVLVEQRFRRVGGDADVQVDVRVI
                     SSSSRDLREEISAGRFREDLFHRLNVVPVRVPGLAERRDDVPEMINYFIERIAEATGL
                     PRRRLAEDAIATLQVHDWPGNVRQLRNNVERMMILAAGDPNEPITADMLPSETPSATH
                     SGAIGTERIIALPLREARELFEREYLNAQILRFGGNISRTAAFIGMERSALHRKLKSL
                     GLAGARGVEEAED"
     gene            complement(8926..11148)
                     /locus_tag="PLO65_01830"
     CDS             complement(8926..11148)
                     /locus_tag="PLO65_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRD27019.1"
                     /translation="MSLSATDPAETGEGAIPAWRTALESRYVVGGGYAVAALLTITGA
                     LLVSSPPAAGPIGPAVKIMLVVLGLNLVLILGLALLVGMRVARLLRDRNVDAGARLHL
                     RFMGLFGLAAVAPAIIVAIFFGGFVLTGVETWFGDKISTLVENSADTARSVVAEQQLI
                     LRDNMTLVAVDLNDASRALETAPVAYGHYLAQISSNTFAAVYVLDHDGRILARAEKPN
                     GPAFVAPPPASFKAADEGIITAETFEAQNVIRGLYRLKAYPDAYLYVARPVGTGIISQ
                     LRDAEAALVALREAANNRDRIKGAFLLTYIETALLVLVGAAWLAIAAANSIAAPVARL
                     VQAAGRVAAGDLNARVDTNDQPEEMAVLSRAFNSMTHDLQVQQVALRTASLDAESRRQ
                     FIETVLLGVSAGVIGLDQAGHLSAANHQAAVLLGVDPEAMAGQALSLIAPELAPVARR
                     AIEIGGEAEEEVDIVRGAETRRLRVRASGAADGIVLTFDDITRLITAQRNAAWKDVAR
                     RIAHEIKNPLTPIQLSAERIKRKYRKEITSDVETFDRCTETIIRQVGDIGRMVDEFSA
                     FARMPAPRFSSHDPAELLRQAVFAQRVASPELTVDMAEPPAGLSLVCDDRMVGQALAN
                     ILKNAAEAVSARGLPTGEGRIAAALVADGDGLSFRIEDNGVGLPVRDRDRLTEPYVTT
                     REKGTGLGLAIVKRILEDHGGELALADATTLTGARVTLNFPNARTTAAPANKEAAHGG
                     "
     gene            complement(11221..12639)
                     /locus_tag="PLO65_01835"
     CDS             complement(11221..12639)
                     /locus_tag="PLO65_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRD27020.1"
                     /translation="MNPASKKILIADDDSSVRLVLSQAFTRLGYQVRATGNAATLLKW
                     VQEGEGDLILTDVMMPDENVFNVLPKIRKERPRLPIIVMSAQNTLLTAVNAADAGAFE
                     YLSKPFDLDDVTAAARRALSRPTDGEAAKAQARAMKDERLPLIGRSAPMQEVYRTVAR
                     LVGADLKVLILGESGTGKELVARALHDLGRRRDGKFVVINLAAVPRERVETELFGRGE
                     GDLGKLVEADGGTLFLDEIGDMPLDAQSRLLRVIDGAEPALNPRTGRAPNVRIIAATN
                     RDLRGLIQQGLFREDLFFRLNEAPVRLPPLRDRVEDIPDLARSFLLRAAREGLPSKTI
                     DQSALERMKTHDWPGNVRELENLVRRVCALYGEDQITARIIERELQDPTPAVGENGPL
                     TLSQLIERHLASHFADQPDGLPAAGLYDRVLEDVERPLIQLTLAATRGNQVRAAEILG
                     LNRNTLRKKIQDLGVDLTRGKR"
     gene            complement(12640..>13454)
                     /locus_tag="PLO65_01840"
     CDS             complement(12640..>13454)
                     /locus_tag="PLO65_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27021.1"
                     /translation="FEADCAASPLGDGAVLLTLHVRGALGAERTSDPAGLRSVVGLGR
                     MLAHEIKNPLAGIRGAAQLLKTGAAAEDQPLAQLIVDETERIRRLVDRMEAFSDDAPP
                     DRAAVNIHSVLDRVRALVANGVAEDLTLRERYDPSLPPTWGDEDLLIQIFLNLVKNAA
                     EAAHMRDDGGGEITISTAWRPGIRVRGPDGRVQTGAPLEVRIQDNGPGVPPHMRDHLF
                     QPFVTSKSFGAGLGLALVAKLVAGHGGLIDFESEPGRTVFRVLLPVAPTGTP"
BASE COUNT         1962 a         4647 c         4649 g         2196 t
ORIGIN      
        1 ggacgacgcg gcccacgaaa tcctgcggac cctgccgcgc ctcgacgatg gcgcgctgat
       61 ggtcctggcc gacagcttcc gcggcccgga aggccaggcg cgcttcgatc tgctgttcga
      121 tcgcctgtcg gcccagatca ggaccatggc cgtgggccag gcgatggccg gcgtggggga
      181 ggggctcgat cgctgggcgt tggcctggga ccagctgcag cggttgccgg accaggtgga
      241 ggggctcaac ctcgaccggg cggacgcctt ctggaccgcg ctgcgcgacc tgcgcgccgc
      301 ggcggcggcc tgaccacgcc atgctgatcg acagccacgt caacctgcat gccccgcagt
      361 tcgccgagga tcgcggggcg gtcatcgacc gggcccgggc ggccggcatc gccctgatgg
      421 tcaatatcag cgacagggtc tcgaccttcg agacgaccta cgccgtcgcc gaggccaacc
      481 ccgacatctg ggcgacggtc ggcacgcatc cgcacgaggc aaaggaaaac ccgcatctca
      541 cggcccgcga catcatcgat ctggccgcgc ggccaaaggt cgtcggcatc ggcgagacgg
      601 gcctcgactt ccactacgac ctgagtccgc gcgaggtgca ggcggcggtg ttccgccagc
      661 acgtgatcgc cgcccgcgag accggcctgc cgctggtcgt gcacacgcgc gaggcggatg
      721 atgtcatggc gcggattctc gaggaggaat acgccgccgg cccgttccgc ctgctgatgc
      781 actgctacac cagcggcgcc gacctggcgc ggagggcggc ggcgctgggg gcgtggttct
      841 cggtctcggg catcgccacc ttcaaggccg cccaggacgt gcgcgacgtc atcgccgata
      901 tgccggccga gcggatcatc gtcgagaccg actgccccta tctggcgccg gtcccgatgc
      961 ggggccgccg taacgagccc gccttcctgc cgcatgtgct ggccaaactg gccgagatcc
     1021 gcggctggac cctcgccgag gccgaacagc acaccgagga cgccttcttc gccctgttcg
     1081 accggatccc gcggccatga gcggcgtgct cgaggtgacc atcctcggca gtggctcgtc
     1141 cggaggcgtg ccgcgcgccg acggaaactg gggcgactgc gatcccgccg aaccgcgcaa
     1201 ccggcgaagc cgctgcggcc tgctgctgcg tcgcaagggg gagggcgccg agaccaccgt
     1261 gctggtcgac acctctccgg acctgcggga acagctgatc gccgccgggg tgcagcgggt
     1321 ggacgccatc ctgttcaccc acgatcacgc cgaccaggtg catggcgtcg atgacgtgcg
     1381 ggccttttat ctgcgccagc gggccaggat tccggcctgg atggacccgc ggacgcgttc
     1441 ggttcttgag cgccggttcg actatatttt caaggatatg ggcggatatc ctgcgatcct
     1501 tgaggcgcgc gacctgccgg cgatgggggg cagttttcgc atcgacggcc cgtcgggtcc
     1561 gattcccgtg acgaccttcg accaggagca cggccacatc cgctgcgtcg gctatcgttt
     1621 cggcgacgtg gcctattcgc cggacgtgaa cggcctgccg gagtcggcct tcgaggccct
     1681 ggcgggcgtc gacacctgga ttgtcgatgc gctgcgccgc acgccgcatc cgacccacgc
     1741 gcatctgcgc cagacgctcg actggattgc gcgggtacgg ccgcggcgcg cgatcctgac
     1801 gaacctgcat atcgatatgg attacaggca gttgtgcgaa gaacttccgc gcgggatcga
     1861 accggcttac gatgggctgt cgcttgagat cggcctgtag cgcacgggca atgccgatcg
     1921 cgcacggcaa atatttccga taatatgcct tatgcgcaat tcggacaaat gggccacggg
     1981 tccatcccgc aactcccccg gtgacgatcc cgtccggcgg gcgtagtctg cccgccgggg
     2041 gcaacaacgg tttatcgcgg agaatgtctc gtgcgcctgg caaccttcct gtccaccacc
     2101 ctgatcgcga tcgccgcggc cttcgccgcc caggcggcgc cgcttggccc ggtggctgtc
     2161 gtccatgtga ccattggccc gcaactgcag gacaaggccg atgagtatgg ccagcaggag
     2221 ctcgactacc tggccgccga cctcaaggat tccgtggagc gggcgctgga gcgcagcggt
     2281 gggctgaaac cgtcgggcgg gacgctcgat ctggtgatcg aggacgccct gccaaaccgg
     2341 ccgaccatgc ggcagatgaa catcaagccc ggcctgtcct acgaaagctt cggcatcggc
     2401 ggcgcgaagg tcggcggcgt gctgacgacg cccgacggtc gccaggtgtc ggtcggctac
     2461 ggctggtatg agaccgacat ccgctggtcc gagggcgcca gcacctggat ggacgcgctc
     2521 aacaccttcg accggttcgc ccggcggctc gtcaccggcg aggccgtgct cgaccattga
     2581 ccgcgatggt cgtccggcgg ccggccggcg tgactccgcc cggccgcttt gtggcgtggc
     2641 gccgcatcct ttcgcttgcg ccttggccga tggcgggctc tgatcgcgtg aacggcgatc
     2701 acccataacg ccgaccccca ctagcgaggc gacatggccg gcggccgacg atccaccctg
     2761 acgctcgcgg cgttcgccgc gccctgcctg cccctggcgg cgttcggcct gccgctgacg
     2821 atcagcctgc cgcactatta ttccgaaacc ctgggacttc aggtttcggc ggtcggcctg
     2881 gccttcctgc tggtgcggct ggtggacatc ggcttcgacc cggtcttcgg caccattctc
     2941 gaccgcaccc gctggtcgtt cggccggttc aagggctgga tggccatcgc cacgccggtc
     3001 ctcatgctgg ccatctacat gctgttcatg gccaggccgg gcgtcaccac aggctacctg
     3061 tggttctggc tgatcgtggt ctatttcggc tattcgatcg ctgtgctgtc gcacacctcc
     3121 tgggcggcca agctgaccac cgactatcac gagcgatcgc gcatctacgc cttctggcag
     3181 accggaaacg tgatcggcat gatcatgatc ctggccatgc cgctggtgct caacctggcg
     3241 tccaggggca tcggccagga gggcatccta cacggccagg gctggttcat cgtcctgctc
     3301 ctgccggcga tggtcgccct gaccctctgg cgcgtgccgg agccccgccc cgtcgaaagc
     3361 accgtccgcg actccggcgg tgcgctggtt tcgcagtccg ccaaccttgg cgactatttc
     3421 cggctgctgc gccgtccgac ggtcatcagg atcctggcgg cggacctgct gaccgggctg
     3481 gcgccgggcg tcgcaggcac gctgttcttc ttctacttca tgcgtgtgaa gcagttcagt
     3541 ttcctcgaat cctcggccct gctgctgatc tatttcgtcg ccgcgatctt cggcgcgatg
     3601 atctggaccc gactggccaa gcggatgggc aagtcgaaag ccctgatcgc cgcctgcgtc
     3661 acctacgcgg cggtgcagtt cgccgtcgtc accctgcccg tcggcaattt ctggctggcg
     3721 acgccgttcc tgatcgcggc cggcatcccc tactccgccg gcgccttgct gcttcggtcg
     3781 atgatggccg actacggcga cgaggaacgg ttgtcgtcag gcgtcgaccg aaccggtctg
     3841 ctgttcgcca tcctgaccgg caccgtgaag atcggctcgg ccctggccgt caccagcctg
     3901 attctgctgg gcgcgctcgg cttcgacgcg gcccatgccg ccaacagcac cccgctgtcg
     3961 atgaccggcc tccaggcgct gtacgccttc gcgccaggca tcgtcggtct gctcgccgcc
     4021 gcggccatga tcggctatcc cctgacggag gcgaagcacg ccgagattct ccgccagctt
     4081 ggagaacgtg acgccgtgtt tccgccggtc gtgcctgagg acgtcgcggg aaccgaaccc
     4141 aatgtcgcaa accccgcagt cacctgaggg cgaccacccg gttttccgcc tgctggagat
     4201 catggatcgc ctgcgcgatc cggcgggcgg ctgcgcctgg gacctcgaac agaccttcgc
     4261 gaccatcgct ccctatacgg tcgaggaggc ctatgaggtc gccgacgcca tcgagcggaa
     4321 cgacctgaag gacctcaggg aggagctggg cgacctgctg ttccaggtcg tcttccatgc
     4381 ccgcatggcg aatgaacagg gcgcgttcga cttcgccgac gtggccgccg ccatcagcga
     4441 caagatgatc cgccgtcacc cgcacgtgtt cggcgatgcg agccgcacgg ccggcgagca
     4501 gaccgcggcc tgggaggtgc tcaagcagca ggagcgggcc ggcaaggcgc gccatggcat
     4561 ccttgacgac gtcccggtcg gcctgcccgc cctgacccgg gcggtgaagc tgtcgaagcg
     4621 cgcggcccgg gtcggcttcg tctggcccag cgtcaaggag gtgctcgaca agctcgatga
     4681 ggaggtcgcc gaactgaaag ccgaggtggc cggcggcgat attgaccggc tgcgcggcga
     4741 gctcggcgac gtgctgttcg tcatcgccaa cctggcccgg acgctcgaca tcgagcccga
     4801 ggacgcgctg cgctccacca acgccaagtt cgtccgccgc ttcggctaca tcgaggaggc
     4861 cctggccgcg cgcggcaaga cgccggagat gtccgacctg gccgagatgg acgccctgtg
     4921 ggacgaggcc aaggcggcgg agcggggctg aggcgcgccc tccccgctct cctccttcgt
     4981 catcgtcgga acaagtccga cgagggcggt cggggtggct acccttccgc ttcggagacc
     5041 ttcgcgtcgg ggaacagccg ctcgaaccgc ccaagcgcct ggccgtcgag gcggacgcgc
     5101 agctgcaggt cgccttcccc gccctcgtga cggccgacca cgcggccgtg gcgatagagc
     5161 caggccagcg cctcgcccgc attcgacgac agctggacgt cgatctccgg cgtctcgtcg
     5221 accaggctgg ccagccggtt gagcagggcc tcgcagccct cgcccgtgat cgacgagacc
     5281 agcagggcgc cgctgcgccg cgccgcgccc tcggcttcgt cccgggcctc gggcgacaga
     5341 agatcggcct tgttccacac ctcgatcagc ttgcggcccg actccggcgt cacgccgagc
     5401 tcggtcagca cggtctcgac atcgcgcttc tgggcctggg tgtcgggacc ggcgatgtcg
     5461 cggacgtgca ggatcacgtc ggcttcctgc acctcctcca gcgtggcgcg gaacgactcg
     5521 atcagctcgt gcggcaggtc cgagatgaaa cccaccgtgt ccgacaggat ggccgggcgg
     5581 ccgccgggca gcttcacatt gcgcagggtc ggatcgagcg tggcgaacag catgtccttg
     5641 gccagcacgc cgccggcggt gatccggttg aacagcgtcg acttgccggc gttggtgtag
     5701 ccgaccaggg cgatggtcgg gtacggtgcc ttcttgcgct gggtccggtg cagggcgcgc
     5761 gtgcggcgca cctcgaccag ttccttcttc atcttgatga tgcggtcggc gatctgccgc
     5821 cggtcgagct cgatctgggt ttcgcccggg ccgccggtcg acccggtgcc gccgcgctgg
     5881 cgttccaggt gggtccaggt ccggaccagg cgcgaacgct cgtattcgag ccgcgccagc
     5941 tcgacctgca acacgccttc gcgggtcctg gcccgccgac cgaagatctc caggatcacg
     6001 ccgctgcggt cgacgacctt gacctgccag gccttttcca gattgcgctg ctgcaccggc
     6061 gagagggcgt cgtcgaccac cacgacgtcg atctcctcga cctcgcacag cagtcgaagc
     6121 tcctcgacct tacccttgcc gaacagcgtc gccggggtcc gctcgcgcag cggcacgatg
     6181 cgcgactcga cgacatcgag gtcgagggcc agcgcaagcc ccgtggcctc ctcaagccgg
     6241 gcctggggat cgcgggagtt cgcctggctg ggccggaccg gatgaagaac cagcgccttt
     6301 tcgacgggat cttcgtgatc gatgagctgg gtcaatcgtc ttgttctgaa ggctcgtaca
     6361 gctggaccgg ctgggcgggc atgatggtgg agatggcgtg cttgtagacc agctgcgact
     6421 ggccgtcgcg acgcagcaac acgcagaaat tgtcgaacca gctgacaacg ccctgaagct
     6481 tgacgccgtt caccaggaag atggtcaggg gcgtcttgga cttgcggacg ctgttaagga
     6541 aggtgtcctg caggttctgt ttcttgtccg tcacggggat tcccctcttg ttggtcggac
     6601 ggcagaaggc cgtccacaca cgcaaagtca gcctaggcgt cgccggacga ccgccgcaac
     6661 ctccgctgag gccgtcgacg ctcaactgtt cgaagcttgg gcgttctgga tatagagcgc
     6721 ccgcaaccgt tgcgccaccg gccccggctt gccggttccg atcggggcgc cgtcgatcgc
     6781 caccaccggc aggatcaggg cgccggcgcc ggtgatgaag gcctcgcggg cgttgcgggc
     6841 ctcctcgggg gtgaaggcgc gctcgtcgag gcggatgttc gcctcgccca gcaggccgag
     6901 caccgccttg cgggtgacgc cgcgcaggat attggcggtc gtgtcgcggg tgcgcagcac
     6961 gccctgggca tcgacaatcc aggcgttcga cgaggcgccc tcggtgacca ggccgtcggc
     7021 gtcgacgaac caggcctccg cggcgccgcg gtcgcgagcg gcggtcttgg ccatggcgtt
     7081 gggcaacagg ccgatggtct tgatgtcgca gcgcccccag cggttctccg gcgtcgtgat
     7141 caccgacacg ccgcgggccg cgcgaacctc cgccgcgatc gggtcgatcg acctggcggt
     7201 gatcacgatc gccggttcag gggccgggat cgggaaggcg tggtcgcgcc gcgcgacgcc
     7261 ccgggtcacc tgcagataca gcaggccgtg ccggacgcga ttgcgcctca gcgcctcgcg
     7321 cagcaccagt tcgagggcct tgcggctcat cggcggccgg atgcccatct cccccaggct
     7381 gcggtcgagc cgctcgaaat ggccgtcgga gtcgaccagc cggccgccca tcaccgccca
     7441 gacctcgtag atgccgtcgg ccagctggta gccacggtcc tcgatatgca ccgccgcctc
     7501 gccgtggcgc acgaagcggc cgttcacata agcccaacgc gacatcagtc ctccgcctcc
     7561 tcgacaccgc gcgcgcccgc cagccccagc gacttcagct tgcggtgcag ggctgaccgc
     7621 tccatgccga tgaaggcggc cgtccgcgag atattgccgc cgaacctcag gatctgggcg
     7681 ttgagatact cgcgctcgaa cagttcgcgg gcctcgcgca gcggcagggc gatgatccgc
     7741 tcggtgccga tggccccact gtgggtggcg ctcggcgtct cggacggcag catgtcggcg
     7801 gtgatcggct cgttgggatc gcccgcggcg aggatcatca tccgctcgac gttgttgcgc
     7861 agctggcgca cgttgcccgg ccagtcgtgc acctgcagcg tggcgatggc gtcctccgcc
     7921 agccggcggc gcggtaagcc ggtggcttcg gcgatccgct cgatgaagta gttgatcatc
     7981 tccggcacgt cgtcgcgccg ctcggccagg cccggcaccc ggacgggcac cacgttgagg
     8041 cggtggaaca gatcctcgcg gaagcggccg gccgagatct cctcgcgcag gtcgcgcgaa
     8101 ctggacgaga tcacccgtac gtcgacctgc acgtcggcgt ccccgcccac gcggcggaac
     8161 cgctgctcga ccagcacccg caggatgcgg ctctgggtct cgcgcggcat gtcggccacc
     8221 tcgtcgagat agagggtgcc gccatgggcg cgttcgaaca cgccgatctt gcccggcttg
     8281 ctgccctcgc cttcctcgcc gaaaagctcc aggtccatcc gttcgggggc catgccggcg
     8341 gcgctgatgg cgacgaactc gccctttgcc cgcgggctgg cggcgtggat catccgggcc
     8401 accagctcct tgccggatcc ggccgggccg gcgatcagca cgcggctgtt ggcgggggcg
     8461 accttggcga tcatctgccg cagctgctgg gccgccagcg agcggccgat cagaccatcg
     8521 ggcgtcaggg cctgagtgcg cagacggcga ttctcgcgcc gcaggctggc cgactcgatg
     8581 gctcgttcga ccatcagcaa cagccggtcg gacttgaacg gcttctcgag gaactcgtag
     8641 gcgccgcgct tgatggcgct gaccgcggtc tcgatgttgc cgtggccgct gatcatgatg
     8701 accggcaggt cggcgtcgag cgccttgacc aggtcgagga gctcgagccc gtccattccc
     8761 ccgccctgca tccagatgtc gagcacgacc agcgtcggct tgcgcgcccg gatggccagc
     8821 agggcctgtt cggaatcggc cgccgttcgc acggcgtagc cttcatcggt gagaatgccg
     8881 gagacgagtt cgcggatatc cgcttcgtca tcgacgacca gaacgtcagc cgccatgggc
     8941 cgcctccttg tttgcgggcg cggccgtcgt gcgcgcgttc gggaaattca gggtcacccg
     9001 ggctccggtc agggtcgtgg cgtccgccaa cgccagctcc cccccgtgat cctcgagaat
     9061 gcgcttgacg atggccagac caaggccggt gcccttctcg cgtgttgtga catagggttc
     9121 ggtcaggcgg tcgcggtcgc gcaccggcag gccgacgccg ttgtcctcga tccggaacga
     9181 caggccgtcc ccgtccgcca ccagcgccgc cgcgatccgt ccctcgccgg tcggcaggcc
     9241 gcgcgccgac accgcctcgg cggcgttctt gaggatgttg gccagggcct ggccgaccat
     9301 cctgtcgtcg cagaccagac tgagcccggc cggcggttcg gccatgtcga cggtcagttc
     9361 cggactggcc acgcgctggg cgaacacggc ctggcgcagc agttcggccg gatcatggga
     9421 cgagaagcgc ggcgccggca tgcgggcgaa ggcggagaac tcgtcgacca tccggccgat
     9481 gtcgccgacc tggcggatga tggtctccgt acagcggtcg aaggtctcga cgtcggaggt
     9541 gatttccttg cggtacttcc gcttgatccg ttcggccgac agctggatcg gcgtcagcgg
     9601 attcttgatc tcgtgggcga tgcgccgggc gacgtccttc caggccgcgt tgcgctgggc
     9661 ggtgatcagg cgggtgatgt cgtcgaaggt cagcacgatg ccgtcggccg cgccgctggc
     9721 cctcacccgc aggcggcggg tctcggcgcc gcggacgatg tcgacctcct cctcggcctc
     9781 gccgccgatc tcgatggcgc gtcgggccac gggcgcgagt tcgggcgcga tcagcgacag
     9841 ggcctgaccc gccatggcct cgggatcgac gcccagcagc acggccgcct ggtggttggc
     9901 ggccgacaga tggccggcct ggtccagacc gatgacaccg gcgctgacgc ccagcagcac
     9961 ggtctcgatg aactggcggc gcgattccgc atcgagactg gctgttctca gggccacctg
    10021 ctggacctga agatcatgcg tcatactgtt gaatgctcgt gataatacgg ccatctcttc
    10081 aggctggtca ttggtgtcga ccctggcgtt gagatcgccg gcggccaccc gcccggcggc
    10141 ctgcacaagc cgggccaccg gcgcggcgat cgaattggcc gcggcgatgg ccagccaggc
    10201 cgccccgacc agcaccagca gggcggtctc gatgtaggtc agcaggaagg cgcccttgat
    10261 ccggtcgcgg ttgttcgccg cctcgcgcag ggcgaccagg gccgcttcgg cgtcgcgcag
    10321 ctggctgatg atgcccgtgc cgaccggccg ggccacatag aggtaggcgt ccggataggc
    10381 cttgagccgg tagaggccgc ggatgacgtt ctgcgcctcg aaggtctcgg cggtgatgat
    10441 gccctcgtcg gcggccttga agctggccgg cggcggggcg acgaaggccg gtccgttggg
    10501 cttctcggcc cgggccagga tgcggccgtc gtggtcgagc acatagacgg cggcgaaggt
    10561 gttggacgag atctgcgcca gatagtgtcc ataggcgacc ggcgcggtct ccagggcccg
    10621 ggaggcgtcg ttgaggtcga ccgccacgag ggtcatgttg tcgcgcagga tcagctgttg
    10681 ctcggcgacc accgatcggg cggtgtccgc cgagttttcg accagtgtgc tgatcttgtc
    10741 gccgaaccag gtttcgacgc cggtcaggac gaagccaccg aagaagatcg ccacgatgat
    10801 cgccggcgcc acggccgcga ggccgaacag gcccatgaag cgcaggtgca accgggctcc
    10861 ggcgtcgacg ttccggtccc gcagcagccg ggcgacccgc atcccgacca gcagggccag
    10921 gccaaggatc agaaccaggt tgagccccag caccaccagc atgatcttga ccgccggccc
    10981 gatcgggcca gccgccgggg ggctcgagac cagcagcgcc ccggtgatgg tgagcagcgc
    11041 cgccacggcg tagccgccgc ccaccacgta gcgggactca agggcggtcc gccaggcggg
    11101 gatcgccccc tcgccggtct cggcggggtc tgtcgcggac aaactcatcg ccctagccta
    11161 gaaccaactg cgcccttcct gcaacagtat tgtggcgggg gcgtgacagc ggggccgggc
    11221 tcagcgcttt ccccgggtca ggtcgacgcc gaggtcctgg atcttcttgc gcagggtgtt
    11281 gcggttcagg ccgaggatct ccgcggcccg cacctgattg cctcgcgtgg ccgccagggt
    11341 cagctggatc aggggccgtt cgacatcctc gagcacccgg tcgtagaggc ctgccgccgg
    11401 caacccgtcg ggctggtcgg cgaagtggct cgccagatga cgttcgatca gctgcgacaa
    11461 ggtcaatggt ccgttctcgc cgaccgccgg cgtcggatcc tgcagttccc gctcgatgat
    11521 ccgggcggtg atctggtcct cgccgtacag ggcgcagacg cgacggacga ggttctccag
    11581 ctcgcgcacg ttgcccggcc agtcgtgggt cttcatccgc tcgagggccg actggtcgat
    11641 ggtcttcgat ggcagcccct cccgggcggc gcgcagcagg aacgagcggg caaggtccgg
    11701 aatgtcctcg acccggtcgc gcagcggcgg cagccgcacc ggggcctcgt tgaggcggaa
    11761 gaacagatcc tcgcggaaca gaccctgctg gatcaggccg cgcaggtcgc ggttggtcgc
    11821 ggcgatgatc cgcacattgg gcgcccggcc ggtgcgcgga ttcagggccg gctcggcgcc
    11881 gtcgatgacc cgcagcaggc ggctctgggc gtccagcggc atgtcgccga tctcgtcgag
    11941 gaacagggtg ccgccgtctg cctcgaccag cttgcccaga tccccctcgc cgcggccgaa
    12001 cagctcggtc tcgacgcgtt cgcgcggcac cgcggccagg ttgatgacca cgaacttccc
    12061 gtcgcgccgg cgacccaggt cgtggagcgc ccgggcgacc agctccttgc ccgtgccgga
    12121 ttcgccgagg atcagcacct tcaggtccgc gccgacgagg cgggcgacgg tgcgatagac
    12181 ctcctgcatc ggggccgagc ggccgatcag cggcagccgc tcgtccttca tcgcgcgggc
    12241 ctgggccttg gccgcctcgc cgtcggtcgg ccgcgacagg gcgcggcggg cggcggcggt
    12301 gacgtcgtcg aggtcaaacg gcttggacag gtattcgaag gccccggcgt cggcggcgtt
    12361 gaccgccgtc agcagggtgt tctgcgcgct catgacgatg atcggcagcc ttggccgttc
    12421 cttgcggatc ttcggcagga cgttgaacac gttctcgtcg ggcatcatca cgtcggtgag
    12481 gatcaggtcg ccctcccctt cctgcaccca tttcagcagg gtcgccgcgt tgccggtggc
    12541 gcgcacctgg tagcccagcc gggtgaaggc ctggctcagc acaaggcgca ccgagctgtc
    12601 gtcatccgcg atgaggatct tcttgctggc ggggttcatt cagggggtcc cggtgggagc
    12661 caccggcaac aggacacgga agaccgtcct gcccggctcg gactcgaaat cgatgaggcc
    12721 gccgtgcccg gcgacgagct tggcgacgag ggccaggccg aggccggccc cgaagctctt
    12781 ggaggtcacg aacggctgga acaggtggtc gcgcatgtgc ggcggcacgc cggggccatt
    12841 gtcctggatg cgcacctcga gcggcgcgcc ggtctggacc cggccatcgg gaccccgcac
    12901 ccgtatgccg ggccgccagg cggtcgagat ggtgatctcg cccccgccgt cgtcgcgcat
    12961 gtgggcggct tcggcggcgt tcttgaccag gttgaggaag atctggatca gcaggtcctc
    13021 gtcgccccat gtcggcggca acgaggggtc gtagcgctcc ctgagagtca ggtcctcggc
    13081 cacgccgttg gccaccagcg ccctcacccg gtccagcacg ctgtggatgt tgacggccgc
    13141 gcggtcgggc ggcgcatcat cggaaaaggc ctccatgcgg tcgaccaggc ggcggatgcg
    13201 ctcggtttcg tcgacgatca gctgggccag gggctggtcc tcggccgcgg cgccggtctt
    13261 gagcagctgg gcagccccgc ggatgccggc cagcgggttc ttgatctcgt gcgccagcat
    13321 gcgcccgagg ccgacaacgg accgcaggcc ggcgggatcg ctggtccgtt cggctcccag
    13381 ggcgccgcgc acatgcaggg tcagcagcac ggccccgtcg cccagcggcg aggcggcgca
    13441 gtcggcctcg aaac
//
LOCUS       DAORNX010000022        13242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3533_length_13242_cov_7.803139, whole genome shotgun sequence.
ACCESSION   DAORNX010000022 DAORNX010000000
VERSION     DAORNX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_13242_cov_7.803139"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="nusB"
                     /locus_tag="PLO65_01845"
     CDS             complement(<1..493)
                     /gene="nusB"
                     /locus_tag="PLO65_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD27022.1"
                     /translation="MTGESKPASLREAYDRLVAATDDKVAPLSAKARRARTVARLAAV
                     QALYQMELAGAGVDAVVREFTDHRFDGDLDGETLAEADETFFSELVHGVVREQGAIDG
                     AIAHRLASGWRLERVDATLRAILRAGGWELMHRPDVPVEVAIDEYVEIAKSFFEGPEP
                     GFVN"
     gene            complement(490..951)
                     /gene="ribH"
                     /locus_tag="PLO65_01850"
     CDS             complement(490..951)
                     /gene="ribH"
                     /locus_tag="PLO65_01850"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRD27023.1"
                     /translation="MPADKVRILIVEGRFYSELSDELLRGAADAITAVGATYDVITVP
                     GALEIPAVIAIAEEAGRGPAGTPYDGYVALGCVIRGETYHFEIVSNESSRGLMDLAVR
                     RGLAIGNGILTVEDEAQAWARARVSEGDKGGGAARACLEVLSIKSRLEPSR"
     gene            complement(955..2076)
                     /gene="ribB"
                     /locus_tag="PLO65_01855"
     CDS             complement(955..2076)
                     /gene="ribB"
                     /locus_tag="PLO65_01855"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRD27024.1"
                     /translation="MSDDSPISPIEDIIEDARNGRPYILVDAEDRENEGDVIIPAQFA
                     TPDQINFMARHARGLICLSITAERARQLRLPPMAAENRESMTTAFTVSIEAREGVTTG
                     ISAHDRSHTVAVAIDPTKGPDDIVSPGHVFPLVARDGGVLVRAGHTEAAIDISRLAGL
                     NPAGVICEIMKDDGTMARLPDLVSFAQLHGLKIGTIADLIAYRRRTERQVERVLETPF
                     TSIHGGDFRMIVYRNTIDRCEHVALVKGRIEADRPTLVRMHQVDMAADMLGFVGARRD
                     YVPAALRAIGEHDGAGVAVFIRDPNPAWLSERYGGEYAAGSQYRSNAIRDYGVGAQIL
                     LDLGVREMILLTSSPFKPAALEGYGLRIVGRQPIETPKE"
     gene            complement(2069..2665)
                     /locus_tag="PLO65_01860"
     CDS             complement(2069..2665)
                     /locus_tag="PLO65_01860"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRD27025.1"
                     /translation="MFTGIVTDVGRVRSVRETNRDRRYEIETAYDLSTVNLGASISHA
                     GCCLTVVEKGDNWFAVEVSAETLSKTTLGAWGEDAPVNLERAARLGDEMGGHIVSGHV
                     DGVGEVLSVEAEGGSHRVRIRAPRPLHRLIAPKGSIAVEGVSLTVNAVEDDVFGLNII
                     PHTWDVTTLGRLRPGSRVNLEIDMLARYLARWQETADE"
     gene            complement(2665..3360)
                     /locus_tag="PLO65_01865"
     CDS             complement(2665..3360)
                     /locus_tag="PLO65_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRD27026.1"
                     /translation="MSLSVTLKLATSLDGRIATASGESRWITGPQAREEVHRLRALHQ
                     AVLVGIETAIADDPELTVRLPGYVGVQPLRVVLDSRQRLPQASKLVREALRLPTIVLT
                     VENPNNRLTDAGVTVLQVAQTEGRPDPKAVLQALQADFDRRLAERFGDRAVGSMAMNV
                     MIEGGGQVAASFLSAGLVDRIEWFRAPIILGDEGRPAIGAFVLKDLAAAPRFRRLSVE
                     TVGDDLWERYERA"
     gene            complement(3357..3824)
                     /gene="nrdR"
                     /locus_tag="PLO65_01870"
     CDS             complement(3357..3824)
                     /gene="nrdR"
                     /locus_tag="PLO65_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRD27027.1"
                     /translation="MRCPFCGHMESQVKDSRPSEDGAAIRRRRLCPECGGRFTTFERV
                     QLRELTIVKRSGRRAPFDREKLVRSISIATRKRPIDPERLDRLVNGIVRQLESMGETE
                     LASHVVGEMVMKALKALDDVAYVRYASVYRDFRHTDDFAQFLSAEGLAEDAED"
     gene            complement(3853..5145)
                     /gene="glyA"
                     /locus_tag="PLO65_01875"
     CDS             complement(3853..5145)
                     /gene="glyA"
                     /locus_tag="PLO65_01875"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRD27028.1"
                     /translation="MNASTPIRPAFFGADLATADPAIAEAIAHELKRQQNQIELIASE
                     NIVSRAVLEAQGSILTNKYAEGYPGKRYYGGCEYIDVLETLAIDRAKALFGAGFANVQ
                     PHSGAQANQAVFMALLQPGDTFMGMDLAAGGHLTHGSPANQSGKWFKPVAYTVRQDTQ
                     YIDYDNVAEVAQREKPKLIIAGGSAYGRAIDFKRFREIADSIGAYLMVDMAHYAGLVA
                     GGAYPNPVTHAHVVTTTTHKTLRGPRGGLVLTNDEAIAKKINSAVFPGLQGGPLEHVI
                     AAKAVAFAEALRPEFKAYAAQVVTNAKALAETLVEAGLAIVSGGTDSHVMLVDLRPKG
                     VTGRDAEASLERAHMTCNKNGIPFDTASFTVTSGVRLGTPAGTTRGFGEAEFRQVGRW
                     IGEVVDGLAANGADGNAAVEARVREQVMALTARFPIYN"
     gene            5312..5743
                     /locus_tag="PLO65_01880"
     CDS             5312..5743
                     /locus_tag="PLO65_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRD27029.1"
                     /translation="MSDQTPENPASDADILACSTEIVAAYVGRNVVGAAALPDLIRTV
                     HASLSGLNGAPAPAPRERQKPAVPVSRSVQHDYIVCLEDGARLKMLKRYLRSRFGLSP
                     EEYRRKWSLPPEYPMVAPAYAERRSDFAKRIGLGTGVRRRS"
     gene            complement(5748..6977)
                     /gene="hemA"
                     /locus_tag="PLO65_01885"
     CDS             complement(5748..6977)
                     /gene="hemA"
                     /locus_tag="PLO65_01885"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HRD27030.1"
                     /translation="MDYKAAFRNAVEQVRDEGRYRVFADLKRHSGDFPRATWTRDDGS
                     EREIVVWCSNDYLGQGQNPVVADAMHAAIDANGTGSGGTRNISGTTHFHVELERELAD
                     LHGKESALLFTSGYVSNEATLSTLYKILPGLIIFSDELNHASMISGIRSGGGHRRIFR
                     HNDLAHLEQLLAEADPEAPKLVAFESVYSMDGDISDIRGTVALAKKYGAMTYIDEVHA
                     VGLYGQRGGGVSERDAIASEIDIIEATLGKAIGVMGGYIAADAVIVDAIRSYASGFIF
                     TTSLPPALTAGALASVRWLKEHNEVRDQHQERAATLKAMMKAAGLPVMESSVSHIVPV
                     MVGDAVHCKMVSDMLLEDHGIYVQPINFPTVPRGTERLRFTPTPFHTDAMMRDLVSAL
                     EKLWARCNIARVGGYAA"
     gene            complement(7204..8103)
                     /gene="mmsB"
                     /locus_tag="PLO65_01890"
     CDS             complement(7204..8103)
                     /gene="mmsB"
                     /locus_tag="PLO65_01890"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HRD27031.1"
                     /translation="MTRIAFIGLGNMGAGMAANQAKAGREVLAFDLSQAAVDKAVAAG
                     CRPAASVAEAVRDADAVITMLPAGPHVRSVYGEQVLADAPKTALLIDCSTIDIDSARA
                     VGEQARGQGFRFADAPVSGGIMAAEAGTLAFMVGCESADYAAFEGILQPMARVVFHAG
                     GSGSGQAAKICNNMLLGISMIGVCEAFALAEKLGLDPERFFEIGSKSSGQCWSLTTYC
                     PWPGPVETAPSNRGYEGGFLSALMLKDLKLAQEAAARAGASTPMGAQAEALYALFDGL
                     GHGQKDFSAMLQLFRGRLDELTA"
     gene            complement(8108..8863)
                     /locus_tag="PLO65_01895"
     CDS             complement(8108..8863)
                     /locus_tag="PLO65_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD27032.1"
                     /translation="MKSRLAIPLAGLFALVLSAAAVAGDIPANIAAAVANPARPEAQR
                     AADASRHPADLLAFAGVKPGDRVSDFMMGGGYFTRILSGAVGPTGKVYAYQAAEFIAY
                     NPDYAKWQDEAATFAPNIAPVRFSLADQAFPEPLDLILTAQNYHDLHLGRATPEQIAA
                     INSEMFAALKPGGVLLLIDHAAADGTGDSLSNKLHRIDIATVKSELTAAGFVLEAESD
                     LLRNPADPRTENVFAPAIRGKTDQFVLKFRKPA"
     gene            complement(8863..9666)
                     /locus_tag="PLO65_01900"
     CDS             complement(8863..9666)
                     /locus_tag="PLO65_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD27033.1"
                     /translation="MKTHARWMALAAVLALGACETVPPPPPPPPADAPVVLPGFLAVA
                     LSDPARPEADRARDATRHPGEVLLFAGLRPGMKVVDLVPGGGYFTRILSRAVGPSGRV
                     YAYVPDELTALAKGRPPSVAAFVGKPPYGNSRMILQTLPAFGAPEKVDMVFTAQNYHD
                     MHLDFMGPADLSVVNRRVFAALKPGGVYIVIDHSAKPGSGLRDSESLHRIDAALVRRE
                     VEAAGFVFDGESRVLSNPRDPLTANVFDPSIRGRTDQFVYRFRKPRSAR"
     gene            complement(9730..10782)
                     /locus_tag="PLO65_01905"
     CDS             complement(9730..10782)
                     /locus_tag="PLO65_01905"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD27034.1"
                     /translation="MTDAPEVLCRVESGVGRITLNRPKALHALTLTMCEDMIDALSIW
                     RHDEVVQCILIDHSGERGFCAGGDIRMLAESGAGDALAARKFFHTEYRLNHLLFTYSK
                     PVVAVMDGVTMGGGVGLAMPSAVRIATERTTFAMPETGIGLFPDVGGGWFLPRLPGRA
                     GLWLALTGARIKAADCCALGIATHYMPSDRVEAFKAALLAHPGSVAAALAAFVEDPGH
                     ARIAAHRADIDRLFGEGDVEAIIANLEVEPGDWAAEQLATLRTKSPQTLKVAFRQLQT
                     GAALDDFAANMAMEYRIGARVVQRHDFLEGVRAVIIDKDNAPKWNPPTVVAVTDAMLD
                     DIFAPLSPDEEWTPLT"
     gene            complement(10779..11918)
                     /locus_tag="PLO65_01910"
     CDS             complement(10779..11918)
                     /locus_tag="PLO65_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HRD27035.1"
                     /translation="MDFSLSDDQRAIQEMAQAFARDELAPHSAKWDEDSHFPIDVMRA
                     AAALGFAGIYVRDDVGGSGLSRLDASIIFEALSYGDVSTAAYMTIHNMASWMIDRFGS
                     DDLRGRYLPRLTTMELIASYCLTEPGSGSDAAAMRTTATKDGDHYVLNGSKAFISGGG
                     VSDVYVVMARTGEPGARGVSAFVVEKGTPGLGFGANERKMGWKSQPTAQVNFDGVRVR
                     ESNRIGGEGEGFRFAMMGLDGGRLNIASCSLGGAAFAVDTAKAYMEARNQFGRPLKDF
                     QALQFKIADMATELEAARLMVRNAADALDRRDPNATKLCAMAKRFATDAGFEVANQAL
                     QLHGGYGYLQDYPLERVVRDLRVHQILEGTNEIMRVIISREMFRQ"
     gene            12028..12513
                     /locus_tag="PLO65_01915"
     CDS             12028..12513
                     /locus_tag="PLO65_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27036.1"
                     /translation="MTRPAGAIRTEAERNLALLGYGLLAVSVFFAGVTALVAVVIAYA
                     LHDAATEQLSSHFRFQIRIFWIGAACVLLAVVGSVVAAFGALGNFLHQAPPADLVRHV
                     VLWGHDVDLSGLRVTPLVVVAATVAVGAALLGMIWTIVAPVIGFIRLASSRHIGQTAR
                     P"
     gene            12510..>13242
                     /gene="hemB"
                     /locus_tag="PLO65_01920"
     CDS             12510..>13242
                     /gene="hemB"
                     /locus_tag="PLO65_01920"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRD27037.1"
                     /translation="MSLPPLAAYPALRLRRPRQADWSRRLVRESVMTPADLIWSMVVH
                     EGEGRIPVASMPGVERLSVADCARAAVEARDLGIPAIAVFPYIDGSTKDAAGSRAADP
                     DGVVSRAVRAMKAAAPEVGIMCDVALDPFTDHGHDGLIVDGRIDNDSTIEKLVEQALV
                     QARAGCDILAPSDMMDGRVGILREALEAEGFQDVMIMSYAAKYASAFYGPYRDAIGSG
                     KLGTLGPADKKTYQQDPANSDEALRE"
BASE COUNT         2002 a         4508 c         4595 g         2137 t
ORIGIN      
        1 cgttgacgaa ccccggctcg gggccttcga agaaggactt ggcgatctcg acatattcgt
       61 cgatggccac ctcgaccggc acgtccggcc ggtgcatcag ctcccagccg ccggcccgca
      121 ggatggcccg cagcgtcgcg tcgacccgct ccagccgcca gccgctggcc aggcgatggg
      181 cgatcgcccc gtcgatcgcg ccctgctccc ggaccacgcc gtgcaccaac tccgaaaaga
      241 aggtctcgtc ggcctcggcc agggtttcgc cgtcgagatc gccatcgaac cggtggtcgg
      301 tgaattcccg caccaccgca tcgacgccgg cgccggccag ctccatctgg tacagcgcct
      361 ggaccgcggc caggcgcgcc acggtgcggg cccgccgcgc cttggcgctc agcggggcca
      421 ccttgtcgtc ggtcgcggcg acgagccggt cataggcttc gcgcagcgac gcgggcttgc
      481 tctcgccggt catcgcgacg gctccagccg gctcttgatg ctcaacacct cgaggcaggc
      541 gcgcgccgcg ccgccgccct tgtcgccctc cgagacccgc gcgcgggccc aggcctgggc
      601 ctcgtcctcg accgtcagga tgccgttgcc gatcgccagg ccgcgccgca ccgcgaggtc
      661 catcagcccg cgcgagcttt cattggagac gatctcgaag tgataggtct ccccccgaat
      721 cacgcagccg agcgcgacgt agccgtcata gggggttccc gccggaccgc ggccggcttc
      781 ctcggcgatg gcgatgaccg cggggatctc aagggcgccc gggaccgtga tcacgtcata
      841 agtcgcgccg accgccgtga tggcgtcggc ggcgccccgc aggagctcgt cggacagctc
      901 ggaatagaag cggccctcca cgatcaggat gcggaccttg tcagcgggca tgcgtcactc
      961 cttgggcgtc tcgatgggct gacgcccgac gatcctgagc ccgtagcctt ccagggcggc
     1021 gggtttgaac ggactggacg tgagcaggat catctcccgc acgcccaggt ccagcaggat
     1081 ctgcgccccc acgccgtagt cgcggatcgc attgctcctg tactggctcc ctgccgcata
     1141 ctccccgccg tagcgttcgg aaagccaggc cgggttcgga tcgcgaatga acaccgccac
     1201 gcccgcgccg tcgtgctcgc cgatcgcccg cagcgcggcc ggcacatagt cgcgccgggc
     1261 gccgacgaag ccgagcatgt cggccgccat gtcgacctgg tgcatccgca cgagcgtggg
     1321 ccggtcagcc tcgatccgcc ccttcaccag cgccacatgt tcgcagcggt cgatggtgtt
     1381 gcggtagacg atcatgcgga agtcgccgcc gtggatgctg gtgaacggcg tctccagcac
     1441 ccgctcgacc tgccgctcgg tgcggcggcg gtaggcgatc aggtcggcga tggtgccgat
     1501 cttcaggccg tgcagctggg cgaacgacac cagatccggc aggcgggcca tggtgccgtc
     1561 gtccttcatg atctcgcaga tcacgccggc ggggttgagg ccggccagcc gcgagatgtc
     1621 gatcgccgcc tcggtgtggc cggcgcggac cagcacgccg ccgtcacggg cgaccagcgg
     1681 aaagacgtgg ccgggggaaa cgatgtcgtc cggtcccttg gtcggatcga tggccacggc
     1741 caccgtgtgg ctgcggtcat gggccgaaat gccggtggtg accccctccc tggcctcgat
     1801 cgagacggtg aaggcggtgg tcatgctctc gcggttctcg gccgccatcg gcggcagccg
     1861 cagctgtcgg gcgcgctccg cggtgatcga caggcagatc aggccccgcg cgtggcgggc
     1921 catgaagttg atctggtccg gcgtggcgaa ctgggcggga atgatcacgt cgccctcgtt
     1981 ctcgcggtcc tcggcgtcca ccaggatgta gggccgcccg ttgcgggcgt cctcgatgat
     2041 gtcctcgatg ggggagatgg ggctgtcgtc actcatcggc tgtctcctgc caccgggcga
     2101 ggtaacgcgc cagcatgtcg atctcgagat tgacccgcga accgggcctg aggcggccga
     2161 gggtggtcac gtcccaggta tgggggatga tgttgaggcc gaaaacatcg tcctcgaccg
     2221 cattgacggt caggctgacg ccctccacgg cgatcgagcc cttgggggcg atcagccggt
     2281 gcagcggccg gggcgcccgg atgcggacgc ggtgtgagcc gccctccgcc tcgaccgaaa
     2341 gcacctcgcc gacgccgtcg acatggcccg agacgatgtg gccgcccatt tcgtcgccga
     2401 gcctggcggc gcgctcgaga ttgaccggcg cgtcctcgcc ccaggccccg agcgtggttt
     2461 tcgacagggt ttcggccgag acctcgacgg cgaaccagtt gtcgcccttc tccaccaccg
     2521 tcaggcagca gccggcgtgg gagatcgacg cgccgagatt gaccgtcgac aagtcgtagg
     2581 ccgtctcgat ttcgtagcgg cggtcgcggt tggtctcgcg cacgctgcgc acccggccga
     2641 cgtcggtgac aattccggtg aacatcaggc cctctcgtac cgttcccaca gatcgtcgcc
     2701 gacggtctcg accgaaaggc ggcggaagcg cggagcggcg gcgaggtcct tgagcacgaa
     2761 ggccccgatg gccggccggc cctcgtcgcc gaggatgatc ggcgcccgga accattcgat
     2821 ccggtcgacc agaccggcgc tgaggaagct ggccgccacc tgcccgccgc cctcgatcat
     2881 cacgttcatg gccatgctcc cgaccgcccg gtctccgaac cgttccgcca ggcgccggtc
     2941 gaagtccgcc tgcaacgctt gcaggacggc cttcggatcc ggacgcccct cagtctgggc
     3001 cacctgaagc acggtgaccc ccgcgtcagt caaacggttg tttggattct cgaccgtgag
     3061 cacgatggtc ggcaaccgca aggcctcccg gaccagcttc gaggcctgcg gcaggcgctg
     3121 gcggctgtcg agcacgaccc gcaacggctg gacgccgaca tagcccggaa gccggacagt
     3181 cagttccgga tcgtcggcga tggccgtttc aattccgacg aggaccgcct gatgcagcgc
     3241 gcgcagccgg tggacctcct cgcgcgcctg ggggccggtg atccagcggc tctcccccga
     3301 ggcggtggcg atgcgaccgt cgagcgaggt ggccagcttg agcgtgacgc tcaaactcag
     3361 tcctccgcgt cttcggccag accttcggcg ctgaggaact gggcgaagtc gtcggtgtgg
     3421 cggaagtccc gatagaccga ggcgtagcgg acataggcca cgtcatcgag cgccttgagc
     3481 gccttcatga ccatctcgcc gaccacatga ctggccagct cggtctcgcc catgctttcc
     3541 agctgacgca cgatgccatt gaccagccgg tcgagccgct cggggtcgat cggccgcttg
     3601 cgggtggcga tcgagatcga gcggaccagc ttctcgcgat caaacggcgc gcgccggccc
     3661 gagcgcttga cgatggtcag ctcccgcagc tggacccgct cgaaggtggt gaagcggccg
     3721 ccgcactcgg ggcacagccg acgccggcgg atggccgcgc cgtcttccga cgggcggctg
     3781 tccttcacct ggctttccat atgaccgcag aacgggcagc gcatgttggg cccctcccgg
     3841 cggccgtccc tattagttgt agatagggaa gcgcgccgtc aacgccatga cctgctcccg
     3901 gacccgtgct tccaccgccg cgttgccgtc ggcgccgttg gcggcaaggc catcgaccac
     3961 ctcgccgatc cagcgcccga cctggcggaa ctcggcttcg ccaaagccgc gggtcgtccc
     4021 cgccggcgtg cccagccgca cgccggacgt gacggtgaag gacgccgtgt cgaacgggat
     4081 gccgttcttg ttgcaggtca tgtgcgcccg ctcgagcgag gcctcggcgt cgcggccagt
     4141 cacgcccttg ggccgcaggt cgaccagcat cacgtggctg tcggtgccgc ccgagacgat
     4201 cgccagcccg gcctcgacca gggtctccgc cagggccttg gcgttggtca ccacctgggc
     4261 tgcgtaggcc ttgaactccg gccgaagggc ctcggcgaag gccaccgcct tggcggcgat
     4321 gacatgctcc agtgggccgc cctgaaggcc ggggaacacc gccgaattga tctttttggc
     4381 gatcgcctcg tcattggtca gcaccaggcc gccgcgcggt cctcgcaggg tcttgtgggt
     4441 ggtggtggtc accacatggg cgtgcgtcac cggattggga taggcgccgc cggcgacgag
     4501 gccagcatag tgggccatgt cgaccatcag gtacgcgccg atgctgtcgg cgatctcgcg
     4561 gaagcgcttg aagtcgatgg cgcggccata ggccgaaccg ccggcgatga tcagcttcgg
     4621 cttctcccgc tgggccacct cggcgacgtt gtcgtagtcg atgtactggg tgtcctggcg
     4681 caccgtgtag gcgaccggct tgaaccactt gcccgactgg ttggccgggc tgccatgggt
     4741 caggtggccg ccggcggcga ggtccatgcc catgaaggtg tcgcccggct gcagcagggc
     4801 catgaacacc gcctggttgg cctgcgcccc ggagtgcggc tggacgttgg cgaagccggc
     4861 gccgaacagg gccttggccc ggtcgatggc cagggtctcg aggacgtcga tgtattcgca
     4921 gccgccgtag tagcgcttgc ccggataccc ctcggcgtac ttgttggtca ggatcgagcc
     4981 ctgggcctcg agcaccgccc gggagacgat attctccgag gcgatcagct cgatctggtt
     5041 ctgctggcgc ttcagttcat gggcgatggc ctcggcgatc gccggatcgg cggtggcgag
     5101 atcggcgccg aagaaggcgg ggcggatggg tgtggaggcg ttcatggtct tacccctggc
     5161 tgcggcgcgc tggctggcgc gccctcttta gcgatcgtgt cggcatgttc aatcggaacc
     5221 cggcgaaggc ggacgcgttg ctcgttgacc ccgccggtcc tggccgaccg gacgggtgtg
     5281 atgacgcatc gccgcgtgag gagtatccgc catgagcgac cagaccccgg aaaatcctgc
     5341 ctccgatgcg gacatcctgg cctgctcgac ggagatcgtg gcggcctatg tcgggcgcaa
     5401 tgtggtgggg gcggcggccc tgcccgacct gatccgcacg gttcacgcct ccctgagcgg
     5461 gctgaatggc gcgcccgcgc cggcgccgcg tgaacgacag aagccggccg tgccggtctc
     5521 ccgatcggtt cagcacgatt acatcgtctg cctggaggac ggcgcgcggc tgaagatgct
     5581 gaagcgctac ctgcgctcgc gtttcggcct gagtccggag gagtatcgcc gcaagtggag
     5641 cctgccgccc gaatacccga tggtcgcgcc ggcctatgcc gagcggcggt ctgatttcgc
     5701 caaacgaatc gggttgggaa cgggcgtgcg gcggcggtct taggctccta ggcggcgtat
     5761 ccgccgacgc gggcgatatt acagcgggcc cacagttttt ccagcgccga aaccaggtcg
     5821 cgcatcatgg cgtcggtgtg gaatggcgtg ggcgtgaacc gcagccgctc ggtgccccgc
     5881 ggcacggtgg ggaagttgat cggctggaca tagatgccgt ggtcctcgag cagcatgtcc
     5941 gagaccatct tgcagtggac cgcgtcgccg accatcaccg gcacgatgtg gctgaccgag
     6001 ctttccatca ccggcagacc cgcggccttc atcatcgcct tgagcgtggc ggcgcgttcc
     6061 tgatgctgat cgcggacctc gttgtgttcc ttgagccagc gaacggacgc cagagcgccg
     6121 gcggtcaggg ccggcggcag cgaggtggtg aagatgaagc ccgaggcgta ggaacggatg
     6181 gcgtcgacga tcaccgcgtc ggcggcgatg tagccgccca tcacgccgat ggccttgccg
     6241 agcgtcgcct cgatgatgtc gatctccgag gcgatggcgt cgcgctcgga aacgccgccg
     6301 ccgcgctggc cgtagaggcc aacggcatgg acctcgtcga tataggtcat ggcgccgtac
     6361 ttcttcgcca gcgcgaccgt gccgcggatg tcggagatgt cgccgtccat tgaatagacg
     6421 ctctcgaacg ccaccagctt gggcgcttcg ggatcggcct cggcgagcag ttgctcgaga
     6481 tgagccaggt cgttgtggcg gaagatgcgc cggtggccgc cgccgctgcg gatgccgctg
     6541 atcatcgagg catggttcag ttcgtcggag aagatgatca ggccgggcag gatcttgtac
     6601 agcgtcgaca gcgtcgcctc gttggagacg tagcccgagg tgaacagcag cgccgattcc
     6661 ttgccgtgca ggtcggccag ttcccgttcg agttcgacat ggaagtgagt cgtgccggaa
     6721 atgttgcgcg tgccgcccga gcccgtgccg ttggcgtcga tggccgcgtg catggcgtcg
     6781 gccacgaccg ggttctggcc ctggccaaga tagtcgttcg agcaccagac aacgatctcg
     6841 cgctcggatc cgtcatcgcg ggtccaggtg gcgcgcggga aatcgccgct gtggcgcttg
     6901 agatcggcga agacccgata gcggccctcg tcgcgcacct gctcgacggc gttgcggaaa
     6961 gcggccttgt agtccatcga cgacgcccgg ccccccatga cggtcgcccc gcccggccgg
     7021 ctccttgttc tctgttccgc gggcttattc gcaccgtcgg actcccgtgt ccacttacgg
     7081 gagtttcatc tggcaaccta ttacgtgaaa cgtcgcagtt ccggcgcgaa cggtcctcac
     7141 gtcctaggca gcaacatggc cggtcgccgg ccgcggagaa ttgatccagc gcaaagccgg
     7201 acgtcaggcg gtcaattcgt cgagtcgccc gcggaacagc tgcagcatgg ccgagaagtc
     7261 cttctgaccg tggcccagac cgtcgaacag ggcatagagc gcctcggcct gggctcccat
     7321 cggcgtcgag gccccggcac gggcggccgc ctcctgggcc agcttcagat ccttgagcat
     7381 cagggcgctg agaaagccgc cctcgtagcc acgattcgac ggcgccgttt ccacagggcc
     7441 cggccaggga cagtaggtgg tcagggacca gcactggcca gacgacttgg agccgatctc
     7501 gaagaaccgc tccgggtcga gaccgagctt ctcggccagg gcgaaggcct cgcagacacc
     7561 gatcatcgag atgcccagca acatgttgtt gcagatcttg gccgcctggc ccgagccgga
     7621 tccgccggcg tggaacacca cccgggccat cggctggagg attccctcga aggcggcgta
     7681 gtcggccgac tcgcagccaa ccatgaaggc cagggtgccg gcctcggcgg ccatgatgcc
     7741 gccgctgacc ggggcgtcgg cgaagcggaa gccctgcccc ctcgcctgtt cgccgacagc
     7801 gcgggcgctg tcgatgtcga tggtcgaaca gtcgatcaac agggccgtct tcggggcgtc
     7861 ggccagcacc tgctcgccat agaccgaccg gacgtgtggc ccggccggca gcatggtgat
     7921 gaccgcgtcg gcgtccctga cggcctcggc gaccgaggcg gcgggccggc aaccggcggc
     7981 caccgccttg tccaccgccg cctgcgacag gtcgaaggcc aggacctcgc ggccagcctt
     8041 cgcctggttt gcggccatcc cggctcccat gttgccgagg ccgataaaag cgatacgggt
     8101 catgggtcta ggccggcttg cggaacttca ggacgaactg gtctgtcttg ccccggatcg
     8161 cgggggcgaa gacgttttcg gtccgcggat cggccggatt gcgcagcagg tcgctttccg
     8221 cctccagcac gaagccggcg gccgtcagct cggatttgac cgtggcgatg tcgatccggt
     8281 gcagcttgtt ggacagggag tcgcccgttc catccgccgc ggcgtggtcg atgagcagca
     8341 acacgccgcc gggcttcaag gcggcgaaca tctcgctgtt gatggcggcg atctgctctg
     8401 gcgtggcccg tcccaggtgc aggtcgtgat agttctgggc ggtcaggatc aggtccagtg
     8461 gctcgggaaa cgcctggtcg gcgagcgaaa accgcaccgg cgcgatgttc ggggcgaagg
     8521 tcgccgcctc gtcctgccat ttggcgtagt cgggattgta ggcgatgaac tcggctgcct
     8581 ggtaggcgta gaccttgccc gtcggcccga ccgccccgga gagaatgcgg gtgaagtagc
     8641 cgccccccat catgaagtcg ctgaccctgt cgccgggctt gaccccggcg aacgccagca
     8701 ggtcggcggg atggcgactg gcgtcggcgg cccgctgcgc ttcaggacgg gccgggttgg
     8761 ccacggcggc ggcgatgttg gccgggatgt cgccggcgac cgccgctgcc gacaggacga
     8821 gggcgaacag tccggcgagc ggaatggcga ggcgcgattt catcagcggg cgctccgggg
     8881 cttgcggaaa cgatagacga actggtcggt ccggccgcga atcgacggat cgaacacgtt
     8941 cgcggtcagg gggtcgcgcg gattgctcag cacccggctc tcgccatcga agacgaaacc
     9001 cgccgcctcg acctcgcgac gaaccagcgc cgcgtcgatg cggtgcaggc tctccgaatc
     9061 acgcaggccg gaacccggct tcgccgaatg gtcgatgacg atgtagacgc cgcccggctt
     9121 gagggcggcg aacacccggc gattgaccac cgacaggtcg gccgggccca tgaaatccag
     9181 gtgcatgtcg tggtagttct gggccgtgaa gaccatatcg accttctccg gcgcgccaaa
     9241 ggccgggagg gtctgcagga tcatccggct gttgccgtac ggcggcttgc cgacgaaggc
     9301 cgcgaccgag ggcggacgcc ccttggccag ggcggtcagt tcgtcgggaa cataggcgta
     9361 gacccggccg ctgggcccaa cggcgcgcga caggatgcgg gtgaagtagc cgccgccagg
     9421 gaccaggtcg acgaccttca tgccgggccg aaggccggcg aacagcagca cctcgcccgg
     9481 gtggcgggtc gcgtcacggg cccggtcggc ctcgggccgg gccggatctg acagggccac
     9541 ggccaggaat cccggcagca ccacaggggc gtcggcgggc ggcggcggcg gaggcggcgg
     9601 aacggtctca caggcgccga gcgccagtac ggccgccagg gccatccaac gggcatgggt
     9661 cttcatgatg cggttttccc cagcaagcgt tttcccccga cccggccttt tcctacgccg
     9721 gttcaggcct caagtcagcg gcgtccattc ctcgtctggc gaaagtggcg cgaagatgtc
     9781 gtcgagcatg gcgtcggtca cggccacgac ggtcggcgga ttccacttcg gcgcgttgtc
     9841 cttgtcgatg atcaccgccc gcacgccctc gaggaagtcg tgccgctgca cgacccgcgc
     9901 gccgatgcgg tattccatgg ccatgttggc cgcgaagtcg tcgagcgccg cgccggtctg
     9961 aagctggcgg aaggcgacct tgagggtctg cggcgacttg gtccggagcg tcgccagctg
    10021 ctcggccgcc cagtcgccgg gctcgacctc gaggttggcg atgatcgcct cgacgtcgcc
    10081 ctctccgaac agccggtcga tgtcggcccg gtgcgcggcg atgcgggcgt gacccggatc
    10141 ctcgacgaac gcggccaggg ccgccgcgac gctgcccgga tgggccagca gggccgcctt
    10201 gaaggcctcg actctgtccg agggcatgta gtgggtggcg atgcccaggg cgcagcagtc
    10261 ggccgccttg atgcgcgcgc cggtcagggc cagccacagg cccgccctgc ccggcaggcg
    10321 cggcaggaac cagccgccgc cgacgtccgg aaacaggccg atgccggttt caggcatggc
    10381 gaacgtcgtc cgttcggtgg cgatgcgcac ggccgagggc atggccaggc cgacgccgcc
    10441 gcccatcgtc accccgtcca tcaccgccac aaccggcttg gaataggtga acagcaggtg
    10501 gttcagccgg tactcggtgt ggaagaattt acgcgccgcg agcgcgtcgc ccgccccgct
    10561 ctcggccagc atgcggatat cgccgccggc gcagaagccg cgctcgccgc tgtggtcgat
    10621 caggatgcac tggaccacct cgtcgtgccg ccagatcgac agcgcgtcga tcatgtcctc
    10681 gcacatggtc agggtcaggg catgcagggc cttgggccgg ttcagagtga tccggcccac
    10741 gccggactcg acgcggcaga ggacttcggg ggcgtcggtc actggcggaa catctcccgg
    10801 ctgatgatca cgcgcatgat ctcgttggtc ccctcgagga tctggtggac gcgcaggtcg
    10861 cggaccacgc gctcgagcgg gtaatcctgc aggtagccgt agccgccgtg cagctgcagc
    10921 gcctggttgg cgacctcgaa cccggcgtcg gtggcgaaac gcttggccat ggcgcacagc
    10981 ttcgtggcgt tcgggtcgcg gcggtcgagg gcgtcggcgg cgttgcgcac catcagccgg
    11041 gctgcctcca gttcggtggc catgtcggcg atcttgaact ggagggcctg gaagtccttc
    11101 agcggccggc cgaactggtt acgggcttcc atgtacgcct tggccgtatc gaccgcgaag
    11161 gcggcgccgc cgagcgagca gctggcgatg ttcagccgcc cgccgtccag gcccatcatg
    11221 gcgaagcgga agccctcgcc ttccccgccg atgcggttgg actcgcgcac gcgcacgccg
    11281 tcgaagttga cctgggcggt cggctgcgac ttccagccca tcttgcgctc gttggccccg
    11341 aaaccgaggc ccggcgtccc cttctcgacc acgaaggccg agacccccct ggcgcccggc
    11401 tcgccggtgc gggccatgac gacgtagacg tcagacaccc cgccgccgct gatgaaagcc
    11461 ttggagccgt tcaggacgta gtggtcgccg tccttcgtgg cggtggtgcg catggccgcc
    11521 gcgtccgacc ccgagcccgg ctcggtcagg caatagctgg cgatcagctc catggtcgtc
    11581 agtcgcggca ggtagcgccc gcgcagatcg tcggagccga accggtcgat catccacgag
    11641 gccatgttgt ggatggtcat gtaggccgcg gtcgacacgt cgccgtagct gagcgcctca
    11701 aagatgatcg aagcgtcgag acgcgagagg cccgaaccgc cgacatcgtc ccggacatag
    11761 atgccggcga agccgagcgc cgccgccgcg cgcatcacgt cgatcgggaa atggctgtcc
    11821 tcgtcccatt tcgccgaatg cggggccagt tcatcccgcg cgaaggcctg ggccatctcc
    11881 tggatcgcgc gctgatcgtc ggaaagggaa aaatccatgc gccctgagcc tcctgtgggg
    11941 caattcgcga ccccggttgt agcgggttga tgacctgacg ccagcggcgc accctgtcaa
    12001 cgaccgcgac tgcgcaggac accgcccgtg accagacccg ccggagccat ccgcaccgag
    12061 gccgaacgca acctggccct gctgggctat ggcctgctgg cggtttcggt gttcttcgcc
    12121 ggggtcacgg ccctggtggc ggtggttatc gcctacgcgc ttcacgatgc ggcgaccgag
    12181 cagctgtcga gccatttccg cttccagatc cgcatcttct ggatcggggc ggcctgcgtt
    12241 ctgctcgccg tggtcggaag cgtcgtcgcc gcattcggcg cgctggggaa ttttctgcac
    12301 caggcgccgc cggcggacct cgtccgccat gtcgtgcttt ggggtcacga tgtcgacctg
    12361 agcggacttc gcgtcacgcc gctggtggtg gtggcggcga ccgtggcggt cggggcagcc
    12421 ttgctcggca tgatctggac gatcgtggct ccggtcatcg gcttcatccg acttgcctcg
    12481 tcgcgccaca tcggtcagac cgcccgccca tgagcctgcc ccctctcgcc gcctatcccg
    12541 ccctgcgtct gcgccgcccg cgccaggccg actggagccg ccgcctggtc cgcgaaagcg
    12601 tgatgacccc ggccgacctg atctggtcga tggtcgtgca cgagggcgag ggccgcattc
    12661 cggtcgcctc catgcccggc gtcgagcggc tgtccgtcgc cgactgcgcc agggcggccg
    12721 tcgaggcccg cgatctgggc atcccggcga tcgcggtctt cccctacatc gacggatcga
    12781 ccaaggacgc cgccggctcg cgggccgccg accccgacgg cgtcgtcagc cgggccgtac
    12841 gggcgatgaa ggctgccgcc cccgaggtcg gcatcatgtg cgacgtggcg ctcgacccgt
    12901 tcaccgacca tggccacgac ggcctgatcg tcgatggccg gatcgacaat gactcgacca
    12961 tcgagaagct ggtcgaacag gccctggtcc aggcccgcgc cggctgcgac atcctcgccc
    13021 cgtcggacat gatggacggc cgcgtcggca tcctgcgcga ggcgctggaa gccgagggct
    13081 ttcaggatgt tatgatcatg tcctacgcgg cgaaatacgc ctcggccttc tacggcccgt
    13141 accgcgacgc gatcggctcg ggcaagctcg gcacgctggg cccggccgac aagaagacct
    13201 accagcagga cccggccaac agcgacgagg cgctgcgcga gg
//
LOCUS       DAORNX010000023        13031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3617_length_13031_cov_7.724568, whole genome shotgun sequence.
ACCESSION   DAORNX010000023 DAORNX010000000
VERSION     DAORNX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_13031_cov_7.724568"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..492
                     /locus_tag="PLO65_01925"
     tRNA            403..492
                     /locus_tag="PLO65_01925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:437..439,aa:Ser,seq:cga)
     gene            complement(668..1087)
                     /locus_tag="PLO65_01930"
     CDS             complement(668..1087)
                     /locus_tag="PLO65_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD27038.1"
                     /translation="MIVATTLPSETEPGSRVATAGRSPGRTFIRDVVQRTGLTFRAVR
                     LYEERGLIVSTRDPLGRRWYDADMSERLAFISLARRAGLSLAEIGDLLKTGDRHGHPR
                     RVSDSLVLLRRRLAELDRQRDAVKACLSELDGGAPDP"
     gene            complement(1090..2523)
                     /locus_tag="PLO65_01935"
     CDS             complement(1090..2523)
                     /locus_tag="PLO65_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD27039.1"
                     /translation="MTEILSLRHAVADRRIAGPDAFAQRNPADPDDLVVTGPGADADI
                     VAAAAAAARAAQPALRTGGIEALGDALDRISDRLMAEAPALSLLAARETGKTIRDARA
                     EVVRAARIFRFFGGEALRNAGERFDSTRRGATVEVAHEPIGVIGAITPWNFPIAIPAW
                     KIAPALAFGNAVVWKPSELSSATASALLEIIAAAGLPPGALNMVLGGSAAGAEVARCD
                     SIDAVSFTGSQAAGHAVRSTATGRGAAVQLEMGGVNGLIVLADADLEVAVETAVNGAY
                     FAAGQRCTATSRIIVEANVADAFAARLEARVAQLGLGDPRHDHTDIGPLASVRQKSRV
                     TEAVDRVAASGATLRFGGAASSHPHAFFPPTLFDDVGDASPLATEEIFGPVAGLFRAA
                     DFDEAMAILNGTRFGLSAGICTRSLARAEAFKRGARAGMCMVNLPTAGVDHHAPFGGI
                     GASSYGPREQGRAARAFFTRMKTIYQA"
     gene            complement(2520..3362)
                     /locus_tag="PLO65_01940"
     CDS             complement(2520..3362)
                     /locus_tag="PLO65_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRD27040.1"
                     /translation="MRPTCRAALVQLCTPDTGTAALDHAMPFVQAAAAGGGELILLPE
                     TCNLMERDQVALGRKVVGEADDPFIAGLRDFAAARHVWILLGSAVVRRPDGLRANRSL
                     LIGPAGEIVARYDKIHLFDADLVNGQSYRESANYEAGTEAVVAETPWGGLGLTICYDI
                     RFPVLHRDLALAGAALIAVPAAFNRQTGAAHWEILLRARAIETGCFILAPAQGGNHAD
                     GEATWGRSTIVAPWGEILAAEAGDQPGLVFADLDLAAVAAARAAIPALVNRRAYTPPR
                     AIHR"
     gene            complement(3362..3826)
                     /locus_tag="PLO65_01945"
     CDS             complement(3362..3826)
                     /locus_tag="PLO65_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD27041.1"
                     /translation="MAIIDNRLAALGLELPSAASPAANYVSFVRSGALVHISGQVSRT
                     AVSTIVGTVGADLSIEQGILAARLCGLNLISQMREACGGDLDRVVRVVKLCGSIQAGP
                     DLEQLPLVMNGCSDLMVEVFGDLGRHARTTVGVYRLPGGVAVEVDAVVEVRD"
     gene            complement(3829..4737)
                     /locus_tag="PLO65_01950"
                     /pseudo
     CDS             complement(3829..4737)
                     /locus_tag="PLO65_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
     gene            complement(4855..5565)
                     /locus_tag="PLO65_01955"
     CDS             complement(4855..5565)
                     /locus_tag="PLO65_01955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRD27042.1"
                     /translation="MTSRIGILKTGNPPADIADQFPTYPDMVRAMLGNGYVFDEFDVE
                     AGELPSPATMVDGYVITGSPAGVYDDLPWIAPFKSWLAATDPATPIVGICFGHQIMAE
                     AFGGKVVKSHKGWGGGLHRYAVTSVEPWMDSSDPFAIAVAHQDQVVELPAGADVVATS
                     DFCPIAAIAYRDRAAITFQCHPEFPIPFAEALVERRRRLGTVTPEQADAARETLARPA
                     DRERVAGWIRAFLARARA"
     gene            complement(5562..6587)
                     /locus_tag="PLO65_01960"
     CDS             complement(5562..6587)
                     /locus_tag="PLO65_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRD27043.1"
                     /translation="MAMPMSRHRPDYAGSTIWNADMPPGDLIHLAEIIAARERIAPFV
                     APTPMLSPPGGPRLKAESLHPCGAFKIRGAFNWLLSLPYAQRAGGVIAPSSGNHAQAV
                     AYAAQRLGIRALAVMPTSSPEAKRAGVRRWGADVKIVDPAIDGSAMAWAATHGPELGL
                     TLIHPYDAREIIAGTGTLGLEILDDVTDPLTVYVPVSGGGLISGVAAAIRSVRSSVRI
                     VGVEPELANDASRSFRDGRRQALPYAQTVRTMADGLRVEQVGQLTWSHIQALVDDMVT
                     VTEDEIGDAVAWVAREGRIVAEPSGAVSVAAARRDAAPDRAVAIVSGGNVDPALYAAL
                     MNSGAVG"
     gene            6683..7660
                     /locus_tag="PLO65_01965"
     CDS             6683..7660
                     /locus_tag="PLO65_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRD27044.1"
                     /translation="MQLSARLETWPIRGVFRIARGEVRAVSCLVVEIEDGGVTGRGEA
                     APEPDVGTTGPLSMALVEGVRSRIEAGVSREALQTLLPACPARNAIDCALWDLEARRL
                     KRSVASLAGLARSRPVQTVYTLSLDAPAAMAEAARARRHMPVLKIKLGGEGDDARVAA
                     VRAAAPDSRLIVDANESWPADDVERLLQRMADLGVAMVEQPLPKGGDERLAAIHRPLP
                     VCADESCSSRDDLPHLVGRYDMINIKLDKTGGLTEALALARAGQEAGLRLMVGCMVGT
                     SLAMAPAHLVAQLAELVDIDGPLLLDRDREPGLRYDGAVVQPPSPSLWG"
     gene            complement(7657..8478)
                     /locus_tag="PLO65_01970"
     CDS             complement(7657..8478)
                     /locus_tag="PLO65_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRD27045.1"
                     /translation="MKLYICADIEGVAGVVTMDHLSPEGFEYEKAREWMTGEVNAAID
                     GARQAGVTEIVVSDSHGNGQNLLLDDLPEDVTVVRSWPRPLEMMQGLEDGGFCGAFLI
                     GQHTGHTDMTGTIAHTISGAVHEIRLNGEPASETRINAAVAGHFNVPVLLATGDEAYV
                     AHVRELLGEAPTVATKSAYTDYCARTITPARSRTLIRAAAERAVLDRQHRSPYRVAGP
                     VTIDMVFKQRAPAQVLSMWPGIEQVSATGVRFIARDMEEASRMIVVATSYDPRAR"
     gene            complement(8475..9590)
                     /locus_tag="PLO65_01975"
     CDS             complement(8475..9590)
                     /locus_tag="PLO65_01975"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRD27046.1"
                     /translation="MSDTVLLNGQAAGARPMTEAEAAILTAEGFEAAGEAIARWPGYA
                     PTPLVELPALARSVSVGRVFYKDEGTRFGLGSFKALGGAYAVQTLVETGDVETVATAT
                     DGNHGRAVAWGARRFGRRAVVYIHETVSEGRADAIRALGAEVVRTPGTYDDSVRQAAR
                     DAAQNGWTVVSDTTWEGYAEIPRLVMYGYGVMVTEALTQAPQGPTHIFVQAGVGALAA
                     SVCAATWHRMGARRPRLVVVEPERAACLFETARQGRLAPATGDADSLMAGLACGEASP
                     LAWDILRTGADAFMTVPDADAIAAMRRLARPAVGDPAITAGESAVAGLCGLLRVCQDD
                     ALRTALGIDGESRVLLFGTEGATDPDLYRRLVNEAVA"
     gene            complement(9587..11053)
                     /locus_tag="PLO65_01980"
     CDS             complement(9587..11053)
                     /locus_tag="PLO65_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRD27047.1"
                     /translation="MRFFIGGLVHETSSFSPIPTSMRKFHASRPAPGASMEELQKLGG
                     ASVYGQWVKRAAEQGHTIAGGPVFSAGPSGLTVQADYEALRDELLESLRSAGPVDGVL
                     LFLHGAQMAVGYRDCEGDVLARARAIVGPTTPIGVELDLHCNISEAMLANATILMACK
                     EYPHTDFGDRAIELFDLIAATAAGRIRPVTSFVRAPMLNLFFTTEQPMRGLVDLTVAM
                     EGKGSVLTTTLAHGFSSADIPEAAASVIVVTDNAREEGDRIAQGLARLFFSLREIGGP
                     KALDVDAVLDAALAEPEGRPVVIADGSDNPGGGAAGDSTFLLRRMLERGVTNAGLAMI
                     WDPTAVKLAMTAGPGARLPMRIGGKTSPMSGDPVDAEVEVLAVHEDPKQIGFSGEKTL
                     GFGAAAALRVGGVDVIVNSYRVQTLSLECFTELGVDPLSKRILVVKSNQHFYAHYAAI
                     AQKVLYSDAPGTTSLNVARRPYRNLARPIWPLDDVAFG"
     gene            complement(11050..12558)
                     /locus_tag="PLO65_01985"
     CDS             complement(11050..12558)
                     /locus_tag="PLO65_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRD27048.1"
                     /translation="MKIFTAVMSHETNVFSPIPTGLDSFRELALYLPGVSSDEDRLKA
                     ARRVEASAGAGDFLRLARERGHEAVLGPVASALPAGAVVRRDYETIRDAIVSSLRAAM
                     PVGAVLLFLHGAQVADGYDDCEGDLISRLREVVGPDVPIGALLDLHGSLTTRMMTSAT
                     ALIFCKEYPHTDYSERAVELFDIIEGAARGELKPHMTRMTVPCLGGLHTRSSPGKDLI
                     DHASSLEGRDGVLSTSVVHGFSLGDVEDSTGNVLVVTDNAPARGEAVARDLAGRFWAI
                     KGSVQSGELPIPDVVEAALAEPRGPVIIAEPGDNPGGGWPADNVDILRALLARDADAA
                     VALVYDPVAVQICSDAGAGAKLSLRIGGKIGAISGPPLDVEAEVTAVNPDARQRTIFG
                     SEPGLFGPAVAIRCGRVDVVLCSVRTQPFSPDVFTELGIDPTKKHVIQLKSSQHFYAF
                     FEPIAAKVLYCNGVGGSVSRVRARGYLKLKRPVWPLDEVTFDATTAPVEQAA"
     gene            complement(12555..>13031)
                     /locus_tag="PLO65_01990"
     CDS             complement(12555..>13031)
                     /locus_tag="PLO65_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HRD27049.1"
                     /translation="WDPAAVRLAMTAGAGATVPLRVGGKAGPLSGPPFDGVARVIATA
                     DDVRQGGPFERSGRPQGGALVEMDGLYIVLSGRRQQCVSPEAFTCLGFDPAQLRLIVV
                     KSMQHFHAHFAALATDIVYASAPGSLTFDLAALPLKNIRRPIWPLDPDPDSEGAEA"
BASE COUNT         2031 a         4603 c         4471 g         1926 t
ORIGIN      
        1 cgaggtcgcc ctggggttcg agacgctcaa ttccggtgag acggccgccg gcgccgcccg
       61 cttcaccggc ggccagggcc gcggagggac cttcctgtga ccggacagat ccgcgcgctc
      121 gcgccgatgt tcgccgagcg ccggaaggac cacctgcccg ggcttcccgg catggcgtgg
      181 gagacggcgc gctcgtgcac gcgggccgcg cgacccggcg gtgggacgcc gaggtgcgcc
      241 atgtcgagac ggaccggatg atcgccctgt tccgccgcac ccgggcggcg ctccggccac
      301 ggccctgaag ccgcaaacgc ccgcttgcgc ttggacggcc acgtcgctag tttccgcgcc
      361 ttcgcactca gggggtcccg acccccacac ccggcgtctt aaggagaggt ggcagagtgg
      421 tcgaatgtac cgcactcgaa atgcggcgtg cctggaaggg caccgtgggt tcgaatccca
      481 ccctctccgc caccgccccg cgtcgtcttg ctcctgtcag gacccgcgcc gccaagcgtc
      541 ggtaatcgcg cgactattcc ctgtacttcc gccgtagcgc ccgcctggac gccgcccttt
      601 cccggcgctt tcgggcgtgt ctggagaccc gtctccgccc gtccgcgatg cagtgcggcg
      661 gggcgggcta agggtccggg gcgccgccgt ccagttccga caggcaggcc ttcaccgcgt
      721 cgcgctggcg atcgagttcc gcgagccttc gcctcagcag gaccagcgag tcggacacgc
      781 gccgggggtg accatgccga tcccctgtct tcaggaggtc gccaatctcg gccagtgaca
      841 ggccggcgcg gcgcgccagg ctgatgaagg ccagccgctc ggacatgtct gcgtcgtacc
      901 atcgccggcc gagaggatcg cgcgtcgaca cgatcaggcc cctctcctcg tagaggcgga
      961 cggctcgaaa tgtcagaccg gtgcgctgaa cgacgtccct gatgaacgtc cggcccggac
     1021 tgcgcccggc cgtcgctacg cgagaccccg gttccgtttc agacggcagg gtggtcgcca
     1081 cgatcatggt caggcctggt agatcgtctt catccgggtg aagaaggccc gagcggcgcg
     1141 gccctgttcc cgggggccgt agctggaagc gccgatgccg ccgaagggcg catggtgatc
     1201 gacaccggcc gtaggcagat tgaccatgca catgcccgcc cgggctccgc gcttgaaggc
     1261 ttccgccctg gccagggacc gggtgcagat tcccgccgag agcccgaatc gcgtcccgtt
     1321 gaggatcgcc atggcctcgt cgaagtccgc cgcgcgaaac aggcccgcca cggggccgaa
     1381 gatttcctcc gtcgccagcg gactcgcgtc gccaacgtcg tcgaacaatg tgggggggaa
     1441 gaaggcgtga ggatggctcg acgccgcgcc gccgaaccgg agcgtcgcgc cgctggcggc
     1501 cacccggtcg accgcctcgg tgacccgcga cttctgccgc acggacgcca gcggaccgat
     1561 gtcggtgtgg tcatgccggg gatcgcccag ccccagctgc gctacccgcg cctcaaggcg
     1621 ggcggcgaag gcgtcggcga cgttcgcttc gacgatgatc cgcgaggtcg ccgtgcaacg
     1681 ctggccggcg gcgaaatagg cgccgttgac cgccgtctcg accgcaacct ccagatccgc
     1741 gtccgcgagc acgatcaggc cattgacgcc gcccatctcg agttggacgg cggcgccccg
     1801 accggtggcc gtcgaccgga cggcgtggcc ggccgcctgc gacccggtga acgagacggc
     1861 gtcaatgctg tcgcatcgcg ccacctcggc gccggcggcg cttccgccga gcaccatgtt
     1921 gagcgcaccc ggcggcagcc cggctgcggc gatgatctcc agcagcgccg atgcggtcgc
     1981 cgacgacagt tccgagggct tccagaccac cgcgttgccg aaggccagcg ccggcgcgat
     2041 cttccatgcc ggaatggcga tcgggaagtt ccacggggtg atcgccccga tcacgccgat
     2101 cggctcgtgc gccacctcga ccgtcgcacc ccggcgggtc gagtcaaacc gttcgccggc
     2161 gttcctcaag gcctcgccgc cgaagaacct gaagattctc gcggcgcgaa ccacctcggc
     2221 ccgcgcatcg cggattgtct tgcccgtttc acgcgccgcg agcagcgaca gggcgggagc
     2281 ctcggccatc aggcgatctg aaatccggtc gagggcgtcg ccgagggctt cgataccgcc
     2341 ggtgcgcagg gcaggctggg cagcccgggc ggccgcggcc gcggccgcaa cgatatccgc
     2401 gtccgccccc gggccggtga cgaccagatc gtccgggtcc gcgggattcc gttgcgcgaa
     2461 ggcgtcaggt cccgcgatgc gccggtccgc gaccgcgtgg cggaggctca ggatttccgt
     2521 catcgatgaa tcgccctcgg cggcgtgtag gcgcggcggt tgacgagcgc ggggatggcg
     2581 gcgcgcgcgg cggcgaccgc cgcgaggtcg aggtcggcga acaccaggcc tggctggtcg
     2641 cccgcctcgg ccgcgaggat ctcaccccac ggcgccacga tcgtggagcg cccccaggtg
     2701 gcctcgccat cagcatggtt cccgccctgg gccggcgcga ggatgaagca gcccgtctcg
     2761 atcgcgcggg ctcgcaacag gatctcccag tgcgcggcgc cggtctgacg attgaaggcc
     2821 gccgggacag cgatcaatgc ggcgcccgcc agggccaggt cccggtgaag aaccggaaaa
     2881 cggatgtcgt agcagatggt caatcccagc ccgccccatg gggtttcggc gacgacagcc
     2941 tcggttcccg cctcgtagtt ggcggactcc cgatagctct ggccattgac caggtccgcg
     3001 tcgaacaggt ggatcttgtc gtagcgcgcc acgatctcgc cggcggggcc gatcagcagg
     3061 ctgcggttcg cccgaaggcc gtccggtcgc cgcaccaccg cggagcccag caggatccag
     3121 acgtgacggg cggcggcgaa gtcgcgcaga ccggcgatga acgggtcatc agcctcgccg
     3181 acgaccttgc gaccgagggc gacctggtcg cgctccatca ggttgcaggt ttcgggcagc
     3241 aggatcagct ctccgccgcc cgcggcggcg gcctgcacga acggcatggc atggtccagc
     3301 gccgccgtgc cggtatccgg cgtgcaaagt tgcacgagcg cggcgcggca cgtcgggcgc
     3361 atcaatcgcg cacctcgacg acggcgtcga cctcgaccgc cacgccaccc gggaggcgat
     3421 agacgccaac cgtcgtgcgg gcgtgcctgc ccaggtcgcc aaacacctcg accatcaggt
     3481 cggagcagcc gttcatgacc agcgggagct gctcgagatc cggcccggcc tggatgctgc
     3541 cgcagagctt gaccacgcgg acaacgcgat ccaggtcgcc gccgcaggct tcgcgcatct
     3601 ggctgatcag attgagcccg caaagccggg cggcgagaat cccctgttcg atcgagagat
     3661 cggcgcccac ggtcccgacg atggtgctga ccgccgtccg cgacacctga cccgagatgt
     3721 ggacgagggc gccggagcgc acgaacgaga catagttcgc agcgggcgag gcggcggacg
     3781 gaagctccag ccccagcgcc gccaggcgat tgtcgatgat ggccatgctc agacggcctc
     3841 gcgcaggggt cgggccgcca gggcggtgcg aatgactgcc tccacctcgg cccggcgcgc
     3901 gccggcgagc ggcaaccgcg gcggccggat gcgatcgttg ccgcgcccga ccgcgacgtt
     3961 gcacatcttg atcgcctgaa cgagatcatg ctcggcatcg agatgcagca gcggcatgaa
     4021 ccaccggtag atccggcgcg cctcggcgag atcgccggct ttcatggcgt cccacagggc
     4081 cagggactcg gcggggaagg cgttggtcag gccggacacc cagccgtccg cgccgagcgc
     4141 cagcgcctcg agcgcgacgt cgtcgaggcc ggcgaacagg acataccggt cgcccagggc
     4201 gttgatcaga tccgtgtagc ggcgggtgtc caccgccgct tacttcagcg cgacgacggt
     4261 gttgtgccgc gccagccgct ccagcgcccg aatggagatg tcgacccggt aggcgggggg
     4321 attgttgtac agcatcaggg gcagcccaga cgcgcccgcg acggtgtcga aatgagcgac
     4381 cagctcgtct tccgtcggga cgtagaccat cgccggcagc accatcagac catccgcgcc
     4441 gagcttttcg gcatcccggg cgtagtccgc ggcggccccg gtcgtgaact cggaaacccc
     4501 tgacaggacc ggcacgcgac ctgcggccac ttcgacggcc gccttgacga ccgaacgttt
     4561 ctcttcggcc gagagcgagt tgttctcacc gaccgtgccc agaacgatga ggccatgcac
     4621 accgtcccgg atgaggcggt cgtagtgctg ctgggtcgcg tccaggtcga tcgacagatc
     4681 cggcgcgaac tgggtggacg aggcgggata gaccccgctc caggcgatgc ggctcatgag
     4741 gtgttccttg cctgcggctt cgatacgcct gatgaaccgc gatcatcgcc cttgggacag
     4801 cccccggggc cgccttcggg ggacctcgcc gcctcttcgt gacacccggc cggatcaggc
     4861 gcgggcacgc gccagaaagg ccctgatcca gcccgcgacc cgctcccgat cggccgggcg
     4921 cgcaagggtt tcccgggcgg cgtcggcctg ttcgggcgtc accgtgccca ggcgccggcg
     4981 acgctcgacg agcgcctcag cgaacgggat cgggaactcg ggatggcact ggaaggtgat
     5041 cgccgcacga tcgcgatagg cgatcgcggc gatggggcaa aagtcgctcg tcgcgacaac
     5101 atccgcgccg gccggcaact ccacaacctg gtcctggtgg gccaccgcga tggcgaacgg
     5161 gtcggacgag tccatccagg gctcgaccga ggtgaccgcg tacctgtgta gccccccgcc
     5221 ccagcccttg tgcgacttca caaccttgcc gccgaacgcc tccgccatga tctggtggcc
     5281 aaagcagatt ccgacgatgg gcgtcgccgg atcggtggcc gccagccagg acttgaaggg
     5341 ggcgatccac ggcaggtcgt catagacgcc cgccggcgac ccggtgatca catagccatc
     5401 gaccattgtc gcgggcgagg gcagttcgcc cgcctcgacg tcgaactcgt cgaagacata
     5461 gccgttaccg agcatggccc gcaccatgtc gggataggta ggaaactggt cggcgatgtc
     5521 cgcgggcggg ttgccggtct tgagaattcc gatcctgctg gtcatccgac tgctccactg
     5581 ttcatcaagg ccgcgtacag ggcggggtcg acattgccgc cgctgacgat cgccacggcg
     5641 cgatccggag cggcgtcacg ccgggccgcc gccacggaca ccgcgccgct gggctcggcc
     5701 acgatccgcc cctcccgggc cacccaggcg acggcatctc cgatctcgtc ttcggtgacc
     5761 gtcaccatat cgtcgaccaa cgcctggatg tgggaccagg tcagttgacc cacctgttcg
     5821 acgcggagtc cgtccgccat ggtccggacc gtctgcgcat agggcagcgc ctgtcttcga
     5881 ccgtcgcgaa agcttcgcga cgcatcattc gccagttcag gttcgacccc gacgatccgc
     5941 acggacgacc gcaccgacct gatcgccgcg gcgacgcccg agatgagccc gccccccgaa
     6001 acggggacat agaccgtcag cggatcagtc acgtcatcca gaatttcgag gccaagggtc
     6061 ccggttccgg caatgatctc ccttgcatca taggggtgga tgagggtcag acccaactcc
     6121 ggaccatgag tggcggccca ggccatggcc gacccgtcaa tcgcgggatc gacgatcttc
     6181 acgtccgcac cccaccgccg gacgcccgcc cgcttcgcct ccggagagga ggtgggcatg
     6241 accgcgaggg ccctgatccc cagccgctgg gcggcatagg cgacggcctg ggcatgattg
     6301 ccgctcgacg gcgcgatcac gccgccggcg cgttgcgcat aggggaggct gaggagccag
     6361 ttgaacgcgc ccctgatctt gaacgcgccg cagggatgga ggctttccgc cttcaatcgc
     6421 ggtccgcccg gcggcgacag catgggcgtc ggggccacga agggcgcgat gcgctcgcgc
     6481 gcggcgatga tctcggccag atggatcagg tcgcctgggg gcatgtccgc attccagatt
     6541 gtcgagccgg catagtcggg gcgatggcgc gacatgggca tggccatccg tcacgacgcc
     6601 atcggcgatc gccattcccc gggcccgccg actggcttcc cgggcgcggc gctgcaagct
     6661 ggacggatcg acggaggatt tcatgcagtt gtcggctcgc cttgagacct ggcccatccg
     6721 cggcgtgttc cgcatcgctc gcggtgaggt tcgcgcggtt tcctgtctgg tcgtcgagat
     6781 cgaggatgga ggggtgactg gacggggcga ggctgctccg gaaccggatg tcggcacgac
     6841 ggggccgctg tcgatggccc tggttgaagg cgtgcggagc aggatcgaag ccggcgtgag
     6901 ccgtgaagcg ctccagaccc tgctgccggc ctgccccgcc cgcaacgcga tcgactgcgc
     6961 cctctgggac ctcgaggcgc gccgcttgaa acggtccgtg gccagcctgg ccgggctcgc
     7021 ccgatcgcgg ccggttcaga ccgtctacac cctcagcctc gacgcgcctg ccgcgatggc
     7081 ggaggccgcg cgggccaggc ggcatatgcc tgtcctgaag atcaagctgg gcggcgaggg
     7141 cgatgacgcc cgggtcgccg ccgtgcgcgc ggccgctccg gacagtcgcc tgatcgtcga
     7201 cgccaacgag agctggccgg ccgacgacgt cgagcgactg ctccagcgca tggccgacct
     7261 cggcgtggcg atggtggaac aaccgctgcc gaaaggcggc gacgagcgtc tcgccgccat
     7321 ccaccgtccc ctgcccgtct gcgccgacga gtcctgcagt agtcgggacg acctgccgca
     7381 tctcgtcggt cgatacgaca tgatcaacat caagctcgac aagaccggcg ggttgacgga
     7441 ggcgctggcg ctggcccgcg ccggccagga ggcgggactg cgcctgatgg tcggctgcat
     7501 ggtcggcacc tcgctggcca tggcgccggc ccacctggtc gcgcagctgg ccgagctggt
     7561 cgacatcgac gggccactgc tgctcgaccg cgaccgggag ccggggctcc gctacgacgg
     7621 cgccgtcgtc cagccgccgt cgccgagcct gtggggctag cgggcgcgcg gatcgtagct
     7681 ggtcgccacc acgatcatcc gggacgcctc ctccatgtcc cgcgcgataa agcggacccc
     7741 cgtggcgctg acctgctcga tccccggcca catggacagg acctgggcgg gcgccctctg
     7801 tttgaagacc atgtcgatgg tgaccggacc agcgacgcga tagggcgacc ggtgctggcg
     7861 atccaggacg gctcgctcgg cggccgcgcg gatcagggtt cgggaccgcg ccggcgtgat
     7921 cgtccgcgcg cagtagtcgg tgtaggcgct cttggtcgcc acggtcggcg cctcgcccag
     7981 cagttcccgc acatgggcca cataggcttc atcgccggtg gcgagcagga ccgggacatt
     8041 gaaatggccg gcgaccgccg cgttgatgcg tgtctcggag gccggctcgc cattgaggcg
     8101 gatctcgtgg accgctccgg agatggtgtg ggcgatcgtg ccggtcatgt cggtgtgacc
     8161 cgtgtgctga ccgatcagga aggcgccgca gaagccgccg tcctccagac cctgcatcat
     8221 ctccagaggc cgcggccagg agcggacgac ggtgacgtct tccggcaggt cgtcgagcag
     8281 gagattctgg ccgtttccgt ggctgtcgct gacgacgatt tcggtcacgc ccgcctggcg
     8341 agcgccgtcg atggcggcgt tgacctcgcc ggtcatccat tcgcgggcct tttcgtattc
     8401 gaagccctcc ggcgagagat ggtccatggt gaccacgccc gcgacgcctt cgatatccgc
     8461 gcagatgtag agcttcatgc caccgcctcg ttcaccagcc gtcggtagag atcggggtcg
     8521 gtcgcgcctt cggtgccgaa cagcaacacc cgcgactctc catcgatgcc gagcgccgtc
     8581 ctgagggcgt catcctgaca gacacgcaaa agaccgcaca agcccgccac cgctgactcg
     8641 ccggccgtga tcgcggggtc gccaaccgcc ggccgggcca ggcggcgcat ggccgcaatc
     8701 gcgtccgcgt cgggcacggt catgaaggcg tccgcgccgg tccgcaggat atcccaggcg
     8761 agcggggagg cttcaccgca ggccagcccg gccatcaggc tgtccgcgtc cccggtggca
     8821 ggcgcgaggc gaccctgccg ggcggtctcg aacaggcagg cggcgcgttc gggttcgaca
     8881 acgaccagtc ggggcctgcg cgcgcccatc cgatgccagg tcgccgcgca gaccgacgct
     8941 gcgagggcgc ccactccggc ctggacaaaa atgtgcgtcg gcccctgcgg cgcctgcgtg
     9001 agcgcttcag tgaccatcac gccgtagccg tacatcacca gacggggaat ttccgcatag
     9061 ccctcccagg tggtgtccga caccaccgtc cagccgtttt gcgcggcgtc ccgcgcggcc
     9121 tggcggacgg agtcgtcata ggttcccggc gtgcggacca cttccgcccc cagcgcccgg
     9181 atcgcgtccg cacgcccctc gctgacggtc tcgtggatgt agacgacggc gcggcgtccg
     9241 aatcgtcgcg cgccccaggc gacggcccgt ccgtggtttc cgtccgtcgc tgtggccacg
     9301 gtctcgacat cgccggtctc gaccagcgtc tggacggcgt atgctccccc gagggccttg
     9361 aaactaccga ggccgaaccg cgtcccctcg tccttgtaga agacccgccc gacgctgacg
     9421 gacctcgcca gggcgggcag ttccacgagc ggcgtcgggg cgtagccggg ccagcgggcg
     9481 atagcctccc ccgccgcctc gaatccttca gccgtcagga tggcggcctc cgcctcggtc
     9541 atcggccggg cgcccgcggc ctggccgttc agcaggaccg tatcgctcat ccgaacgcga
     9601 cgtcgtccag cggccagatc ggccgcgcca ggttccggta ggggcgccgc gcgacattga
     9661 gcgaggtcgt ccccggcgcg tccgaataca gcaccttctg ggcgatggcc gcgtagtggg
     9721 cgtagaagtg ctggttggat ttgacgacca ggatgcgctt tgacaggggg tcgaccccca
     9781 gttcggtgaa gcactcgagg ctgagggttt ggacccggta gctgttgacg atcacatcga
     9841 ccccgcccac ccgcagggcc gccgcggcgc cgaatcccag ggtcttctcg cccgagaatc
     9901 cgatctgttt cggatcctcg tgaacggcca gaacctcgac ctcggcatcg acgggatccc
     9961 ccgacatcgg gctggtcttg ccgccgatcc gcatcggcag ccgcgcgccc ggccccgcgg
    10021 tcatggccag ctttaccgcg gtcggatccc agatcatcgc caacccggcg ttggtgacgc
    10081 cgcgctccag catgcgccgc aggaggaagg tcgagtcgcc cgccgcgccg ccacccggat
    10141 tgtccgaccc atcggcgatg accaccggac gcccttccgg ttcggcgagg gccgcgtcga
    10201 gcacggcgtc cacatcgaga gccttggggc cgccgatctc gcgcagggag aagaacagcc
    10261 gcgccagtcc ctgggcgatg cgatcgccct cctcacgcgc gttgtcggtg accacgatga
    10321 cgctggccgc cgcctcggga atgtcggccg agctgaaacc atgggccagg gtggtcgtca
    10381 gcacgctgcc cttgccctcc atcgccaccg taaggtcgac gagcccccgc atcggctgtt
    10441 ccgtggtgaa aaacaggttc agcatgggcg cccgcacgaa ggacgtcacc ggcctgatcc
    10501 gacccgccgc ggtcgccgcg atcagatcaa acagctcgat ggcgcggtcc ccgaagtcgg
    10561 tgtgcggata ctccttgcag gccatcagaa tggtcgcgtt ggccagcatc gcttcggaga
    10621 tgttgcagtg gaggtccagt tcgacgccga tcggcgttgt cggcccgacg atcgcccggg
    10681 cccgggccag cacatcaccc tcgcagtccc gatatccgac cgccatctgg gcgccgtgga
    10741 ggaacagcag cacgccatcc accggcccgg cgctgcgcag gctttcgaga agctcgtcac
    10801 gcaaggcctc gtagtcggcc tgcaccgtca gaccactcgg accggcggaa aagaccggcc
    10861 cgccggcaat cgtgtgaccc tgctccgcgg cccgtttgac ccactgccca tagaccgagg
    10921 cgccgcccag cttctggagc tcctccatcg acgccccggg cgcgggacgg gacgcatgga
    10981 actttctcat gctggtggga atgggcgaga agctcgaggt ttcgtgcacc aggccgccga
    11041 tgaagaacct catgccgcct gctccaccgg ggcggtcgtc gcgtcgaagg tcacctcgtc
    11101 gagcggccag accggccgtt tcagtttcag atagcccctc gcccgcaccc ggctgacact
    11161 gccgcccacg ccattgcagt agagcacctt ggccgcgatc ggctcaaaga acgcatagaa
    11221 gtgctgtgac gacttgagct ggatgacgtg cttcttcgtc gggtcgatgc ccagctcggt
    11281 gaacacgtcc ggactgaacg gctgcgttcg gaccgagcag agcaccacgt ccacgcggcc
    11341 gcagcggatc gccaccgcgg ggccgaagag cccgggttcg gagccgaaga tggtgcgttg
    11401 gcgggcatcg ggattcacgg ccgtcacctc ggcctccaca tcgagcggcg ggccggagat
    11461 cgcgccgatc ttgcccccga tgcgcagcga caatttcgcg cccgccccgg catccgagca
    11521 gatctgcacg gccaccggat cgtagaccag ggcgaccgcg gcgtcggcgt cgcgcgccag
    11581 cagagcccgc agaatatcga cattgtccgc cggccagccc ccgccgggat tgtcgccagg
    11641 ttcggcgata atgaccgggc ctcgtggttc ggccagcgcc gcctcgacga cgtcgggaat
    11701 cggcagctcg ccggactgga ccgacccctt gatcgcccag aagcgtccgg ccaggtcccg
    11761 ggcgaccgcc tcgccccggg cgggagcatt gtcggtcacg accagcacat tgcccgtcga
    11821 gtcctcgaca tcgcccagtg aaaagccatg aacgaccgat gtcgacagga cgccgtcgcg
    11881 accctcgagg gagctggcgt ggtcgatcag gtccttgccc ggcgagctcc gcgtatgcag
    11941 gccaccgagg cagggcacgg tcatccgggt catgtggggc ttcagctcgc cgcgcgcggc
    12001 gccctcgatg atatcgaaca gttccaccgc gcgttcgctg tagtctgtgt gcggatactc
    12061 cttgcagaag atcagggccg tagccgacgt catcatgcgc gtggtcaggg agccgtgcag
    12121 gtccagcagc gcgccgatcg gcacgtcagg cccgaccacc tcgcggaggc gcgagatcag
    12181 atcgccctcg cagtcgtcgt agccgtcggc gacctgcgca ccgtggagga acaggagcac
    12241 tgcgccgacc ggcatcgcgg ccctcaggga gctgacgatg gcgtcacgaa tggtctcgta
    12301 gtcgcgccgc acaaccgctc cggcgggcag cgctgacgcc accggaccga gcaccgcctc
    12361 gtgcccccgt tcccgagcga ggcggagaaa gtcgcccgcg cccgccgagg cctcgacccg
    12421 cctggccgct ttcagccggt cttcgtcgct gctgacgcct ggcaggtaca gcgccagttc
    12481 acggaagctg tcgaggccgg tggggatcgg cgagaagacg ttggtctcat gggacatgac
    12541 ggcggtgaag atcttcatgc ctcagctcct tcggagtcgg gatcggggtc gagcggccag
    12601 atcggccggc ggatgttctt cagaggcagc gcggccagat cgaaggtcag gctgccgggc
    12661 gcggaggcgt agacgatgtc cgtcgccagc gcggcgaaat gcgcgtgaaa gtgctgcatg
    12721 gacttcacca cgatcagccg cagctgggcc gggtcaaacc cgaggcatgt gaaggcctcc
    12781 ggactgacgc attgctgccg ccgccccgac agcacgatgt acagcccgtc catttcgacc
    12841 agtgcgccac cctgggggcg tccggaccgc tcgaatggcc ctccctgccg gacgtcgtcg
    12901 gccgtggcga tgaccctggc caccccgtcg aagggtgggc cggacagggg gcctgccttg
    12961 ccgccgacgc gcaggggaac cgtcgcgccg gcgccggccg tcatcgccag ccgcacggcg
    13021 gcggggtccc a
//
LOCUS       DAORNX010000024        12882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3673_length_12882_cov_7.329305, whole genome shotgun sequence.
ACCESSION   DAORNX010000024 DAORNX010000000
VERSION     DAORNX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_12882_cov_7.329305"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLO65_01995"
     CDS             complement(<1..242)
                     /locus_tag="PLO65_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HRD27050.1"
                     /translation="MMSTRLMCAAALIAAAGLGGCTSTSQALGLTKVTPDEFRVVTKA
                     PLVVPPDYALRPPAPGEPRPQELQPESAARTALAGQA"
     gene            complement(338..904)
                     /gene="lspA"
                     /locus_tag="PLO65_02000"
     CDS             complement(338..904)
                     /gene="lspA"
                     /locus_tag="PLO65_02000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRD27051.1"
                     /translation="MTDAEDTPVAEPVSMAEATLEPAAAESPAAPARLQGPTAYAICL
                     AVIVADQLIKQWILYAFQLPVLGSVQVLGPFHLTMVWNQGVSFGLFRGEAEWVRWALA
                     AFSLGVAGFLGVWARQVERPLLGGAIGLVMGGAIGNLIDRVRFGAVADFIDFKALHFP
                     WVFNIADSAITIGVVLLLIDSIGRDAKR"
     gene            complement(1041..4022)
                     /gene="ileS"
                     /locus_tag="PLO65_02005"
     CDS             complement(1041..4022)
                     /gene="ileS"
                     /locus_tag="PLO65_02005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRD27052.1"
                     /translation="MADDAAPQRDYRETVFLPETAFPMRAGLPTLEPKILQAWTDQDL
                     YNTVRKARQAAGAPLFVFHDGPPYANGAIHIGHSLNKSLKDFVVRSRFALGFDVDYVP
                     GWDCHGLPIEWKIEEEYRGKGRRKDEVPTEEFRTRCREYAAGWIGVQMAEHKRLGTLA
                     DYEHRYATMDFTSEATIVAEFHRFMMSGQLYRGSKPVMWSPVEGTALADAEVEYHDHT
                     SPTVWVKFPVDWVAVDLPPAVPGEPQIDLTAFAADAGAASDASVVIWTTTPWTIPANR
                     AVSYNPRIAYGVYRVEALEEGLEFAPWAASGDKLVVADKLAEEVFKAAKVAKWTRLAP
                     VDCSKLTLRHPLAKLDEGYGFPVPMLAGDHVTDDAGTGFVHTAPGHGADDYLIWLKSK
                     AQLEAAGIDTTIPETVGPDGAYYDHVPLFAGLKVLETEGKKLGKFGPANPAVIEKLIE
                     AGNLLARGRLEHSYPMSWRSKAPVIFRNTPQWFIRMDEPLADGRTLRETALQSIDATA
                     FHPDMGRNRIRGMVETRPDWLISRQRAWGTPLAMFVDRDTGEPLRDEAVNKRIVDLVA
                     KEGADAWFLRPASDFLGEHDPERFEKINDILDVWFDSGATHAFTIEGRADSHWPADLY
                     LEGSDQHRGWFQSSLLESSGTRGRAPYKAVVTHGFTLDENGVKMSKSLGNTIEPQVII
                     KESGAEILRLWAATVDYMEDQRIGKTILATVTDSYRKLRNTLRYLLGALNGFEEAERV
                     AVADMPPLEQYILHRLWQLDGEVRAAYETYRFSEVVRPLLEFCQGDLSTFFFDIRKDA
                     LYCDRPDSLRRRACRTVMDAVFERLTIWLSPLIPFTTEEAWTTRFPDAGSNSLRVFPG
                     TPADWRNDAEAARWGKVEQVTGVVTGALEVERREKRIGSSLEASPSVHVADAALLAAF
                     EGLDAAEIFRTSGVTLAAGEGGPSSFRMTDMKAVAVDPRPADGEKCARCWRVLPEVTG
                     AGRLCLRCDDAVAAWDKTHAA"
     gene            complement(4092..4136)
                     /locus_tag="PLO65_02010"
     CDS             complement(4092..4136)
                     /locus_tag="PLO65_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27053.1"
                     /translation="MTSVRKISRIIRPA"
     gene            complement(4323..5399)
                     /locus_tag="PLO65_02015"
     CDS             complement(4323..5399)
                     /locus_tag="PLO65_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD27054.1"
                     /translation="MLKARKYERFARARRPAATRVKPESFAFPPDASFVLDTRVEPRL
                     VRSEMDLLDAYSDAVVRAVESVGPAVVRVAPITTDGSFAGEGSGFFVSADGLIVTNSH
                     VVGGFSRLAVITAEGRALTARPIGDDVDTDIAVLKVEQPVKAPFARLGDSKKLRRGQL
                     VIAIGAPLGFEATVTTGVVSALGRSLRAEKGRLIEDLIQTDAALNPGNSGGPLVSSNG
                     EVIGINTAVIVGAQGLCFAVASNTASFVMAEIAKHGHVRRGTIGVVAQQTPIPPAIAK
                     SAGVTQPYAVWVASVDAGGPAEKAGIREGDLIVAADGRLITGLDDLLRALGPASIDKP
                     LVLDIVRAGVRMKVTVTPKARKKS"
     gene            complement(5499..>6159)
                     /gene="ribF"
                     /locus_tag="PLO65_02020"
     CDS             complement(5499..>6159)
                     /gene="ribF"
                     /locus_tag="PLO65_02020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRD27055.1"
                     /translation="YVLAFDFEMANFSDRAFVEQVLHEGLGVRHVAVGFDISFGKGRT
                     GSPEAMKAYGAELGFTVSVAEAVGEGAEKYSSTAVRAALRDGHPEAAAAILGRPFAIE
                     GVVRRGQQLGRQLGFPTANVFMVDYVVPRLGVYATRTRLPDGRVLPGVANIGNNPTTG
                     EVETRLEVWLFDFDEDLYGQVIETQLIGFLRPEEKFASIDEMVVQIRRDEATARAILQ
                     K"
     assembly_gap    6160..6214
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6215..6429)
                     /locus_tag="PLO65_02025"
     CDS             complement(<6215..6429)
                     /locus_tag="PLO65_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HRD27056.1"
                     /translation="MSLRIVHGWKHLAPADRGAAVALGNFDGVHLGHQQVIAGAATAA
                     RAIGAPLAVITFDPHPRRLFHPDEPAFR"
     gene            complement(6429..6851)
                     /locus_tag="PLO65_02030"
     CDS             complement(6429..6851)
                     /locus_tag="PLO65_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD27057.1"
                     /translation="MAGLFFEDLSVGQSAELTRTVDEKAIQAFAEVSGDFNPVHLDEA
                     FAATTPFKTRIAHGMLSAAYISALIAGKLPGPGAIYLGQTLSFRRPVKIGDAVTVTAT
                     ITALDPEKARATLATVCSVGGKAVLEGEATIMVPRRPA"
     gene            complement(6989..7849)
                     /locus_tag="PLO65_02035"
     CDS             complement(6989..7849)
                     /locus_tag="PLO65_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HRD27058.1"
                     /translation="MTTLPAGLADLADNYDVLLSDVWGVIHNGRESFADACAALARFR
                     AERGPVVLISNSPRPAHDVVPQLDALGVPREAWSTLVTSGDATRLLLGQRAPGPAWVI
                     GPERDNTLYAGLGLSFADVADAAFIACTGPFDDEVETPEDYRERFTAPAARGLTMICA
                     NPDRVVQRGSKLIYCGGALADLYKDMGGPVLMAGKPYAPIYDLSLAEAARLLDRDVDM
                     ARVLCIGDGVHTDVQGAEQQGLDCLFVARGIHGDAAMGPDGRLSAPAVDALLAAEGVR
                     ARYAMADLVW"
     gene            complement(7929..8624)
                     /locus_tag="PLO65_02040"
     CDS             complement(7929..8624)
                     /locus_tag="PLO65_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD27059.1"
                     /translation="MKPDVLDWKRIAADLDARGWAVTGRLLDDDACAALAGLYEQEAG
                     FRSRVVMARHGFGQGEYRYFSYPLPDAVQVLREGLYPPLAAIANGWAERLGEARRFPP
                     TLAGMLGRCHAAGQLRPTPLLLKYGPGDYNCLHQDLYGEHVFPLQAAFLLDQPGADFE
                     GGEFVIVEQRPRQQSAPAVVPLTRGCAVIFAVRERPHDGTRGVHRRILRHGVSEVRSG
                     RRRTLGVIFHDAS"
     gene            complement(8621..8845)
                     /locus_tag="PLO65_02045"
     CDS             complement(8621..8845)
                     /locus_tag="PLO65_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRD27060.1"
                     /translation="MTYDNDDARWAAMQRRDRDADGVFLFAVKTTGVYCRPSCSGRPL
                     RRNVAFYATAGAARADGYRACRRCKPDAAA"
     gene            complement(8936..10195)
                     /locus_tag="PLO65_02050"
     CDS             complement(8936..10195)
                     /locus_tag="PLO65_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD27061.1"
                     /translation="MRRLTLALLSGAYFCITLAVAFLMWRYGYGAGAGIAALIGGLGL
                     AFAFHGLIGGAINTARVTGELDNIREAHLILARQIEKIDGRLNEVVESVTADALRRSE
                     ELTSEVHSLEDAVQKMAEKMEAQLSSRVAAAAPHALGRGPQSAALLDTVKDALAGNRV
                     DLYLQPVVSLPQRRTVFYESFSRLRDETGRVMMPAEYLSVAEPEGLVAAIDNLLLFRC
                     VQIVRRLARQDRKVGIFCNISLASLSDETFFPQFLDFLSANKDMAGALIFELGQAAFE
                     RRGAVEARHMARLADLGFRFSLDKVVDLDLDFQDLARSDIKFIKVGAQMLLDQLEESE
                     GRLVIRSLPDLHAADFARLTRRYGVEVIIEKVESERQIVDILELDMAFGQGHLFGEPR
                     AIRDAILAEADPPADFMRTTLRRASGW"
     gene            10347..10676
                     /locus_tag="PLO65_02055"
     CDS             10347..10676
                     /locus_tag="PLO65_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516121.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRD27062.1"
                     /translation="MSEAAVLEPQSPPTLTSADIIDVGPLSAAGVGGRGVIVRVSAFE
                     GALPAAELERRLLELGFVEGAVVEILHEGAFGRDPMALRLDDMRVAIRRREAAAIQVR
                     FDSRAGA"
     gene            10673..12547
                     /locus_tag="PLO65_02060"
     CDS             10673..12547
                     /locus_tag="PLO65_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRD27063.1"
                     /translation="MTHASLAPARVALVGNPNSGKTALFNALTGSRQKVANYAGVTVE
                     RKEGVLTTPAGRVIQVLDLPGTYSLRARSPDEAVTRDAVLGRLAGEVPPDVIICVADA
                     TNLRLVLRLALELRQVGRPFVLALNMYDIAQRQGLRIDLDGLSREIGAPIVTTVATRK
                     RGIEELVARAEALADTMPVGENVWHEPSAAEIRHAHAEAQRILKAHVRPPERPDTWTS
                     RIDGVLLHPVAGLLILSAIMFVMFQAVFAWATPVADLIEAGFTWLGGAVATVTPEGLL
                     RSLLVDGLIAGVGSVLVFLPQILTVFFFILLLEDSGYMARAAFLMDRIMGAAGLHGRA
                     FIPLLSSHACAIPGVMAARVIDQKHDRLTTILIAPLMTCSARIPVYTLIIGALIPRTT
                     VWGFLNLQGLVMFGLYAAGILSGLIVATVIRTLFWRSASEPFMMELPTYRWPDPENVA
                     RNLMQRAMIFINRAGRIILPLMVLVWVLSTFPYPPAGATGPAIDYSFAGMIGHVIQPL
                     LAPIGFNWQMAVALVPGMAAREVAVAALGTVYAVGGADGAGQATLASILHAQWSLATG
                     LSFLAWYVFAPQCVATLGVVKRETNGWLWPTVMFGYMFALAYLASLATYWGAVALGWG
                     "
     gene            complement(12551..>12882)
                     /locus_tag="PLO65_02065"
     CDS             complement(12551..>12882)
                     /locus_tag="PLO65_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27064.1"
                     /translation="DFPAFIVNRVLVPMINEAIYTLYEGVGTVESIDTAMKLGANHPM
                     GPLELGDFIGLDTVLSIMNVLHEGLADSKYRPCPLLVKYVEAGWLGKKVGRGFYDYRG
                     ETPVPTR"
BASE COUNT         1907 a         4448 c         4490 g         1982 t
ORIGIN      
        1 gcctggccgg ccagggccgt gcgggccgcg ctctcgggct gcagctcctg cgggcgaggc
       61 tcgccggggg ccggcgggcg cagggcatag tcgggcggaa ccaccagcgg ggccttggtc
      121 accacgcgga actcgtcggg cgtcaccttg gtcaggccca gcgcctggga cgtggaggtg
      181 cagccgccca gaccggcggc ggcgatcagc gcggcggcgc acatcaggcg ggtagacatc
      241 atagagcctt gctcctcagg accgttcggc cagctggccg cccccggccg taacaggcgc
      301 tctcgatacg ccaaaagtga ggcgaacggc aactgcgtta ccgtttcgcg tccctgccga
      361 tgctgtcgat cagcagaagc accacgccga tggtgatggc gctgtcggcg atgttgaaca
      421 cccaggggaa atgcagcgcc ttgaaatcga tgaagtcggc caccgcgccg aaccgcaccc
      481 ggtcgatcag gttgccgatc gcgccaccca tgaccaggcc gatggccccg cccagcagag
      541 gccgctcgac ctgtcgcgcc cagacgccga gaaagccggc gaccccgagg gaaaaggccg
      601 ccagggccca gcggacccat tcggcctcgc cgcggaacag gccgaagctg accccctggt
      661 tccagaccat cgtcaggtga aacgggccca gaacctggac gctgcccagg accggcagct
      721 ggaaggcgta gagaatccac tgcttgatca gctggtcggc gacgatcacc gccagacaga
      781 tggcgtaggc cgtcggcccc tgcaggcgcg cgggggcggc cggcgattcg gcagcggccg
      841 gctccagcgt cgcttcggcc atcgagacgg gctcggcgac aggggtgtct tcggcgtcgg
      901 tcacgtcgga actcattccc aatccctgcc ggcctctacc aacaccgtca tcctcgggct
      961 tgtcccgagg acccatgcac actcccggcc gacggtgttc atgggtggtc gggacaagcc
     1021 cgaccatgac ggttgaagta tcaggccgcg tgggtcttgt cccacgccgc gacggcgtcg
     1081 tcgcagcgca ggcagaggcg gccggcgccg gtgacctcgg gcagcacccg ccagcagcgg
     1141 gcgcatttct ctccgtcggc cgggcgcgga tcgacggcca cggccttcat gtcggtcatg
     1201 cggaagctgg acgggccgcc ctcgcccgcc gccagggtca cgccgctggt gcggaagatt
     1261 tcggccgcgt ccaggccctc gaaggccgcc aggagcgccg cgtcggccac atggaccgac
     1321 ggcgaggctt ccagcgacga gccgatacgc ttctcgcgcc gctcgacctc cagcgcgccg
     1381 gtgaccacgc cggtgacctg ctcgaccttg ccccagcgcg cggcctcggc gtcgttgcgc
     1441 cagtcggccg gggtcccggg gaatacgcgc aggctgttcg accccgcgtc ggggaagcgg
     1501 gtcgtccagg cttcctcggt ggtgaacggg atcagcggcg agagccagat ggtcagccgc
     1561 tcgaacaccg cgtccatcac cgtgcggcag gcgcggcggc gcaggctgtc gggccggtcg
     1621 cagtagagcg cgtccttgcg gatgtcgaag aagaaggtcg acaggtcgcc ctggcagaac
     1681 tccagcagcg gccggaccac ctcgctgaag cggtaggtct cgtaggccgc gcgcacctcg
     1741 ccatccagct gccacagccg gtgcaggatg tactgctcca gcggcggcat gtcggccacg
     1801 gccacgcgct cggcctcctc gaagccgttc agggcgccga gcaggtagcg cagggtgttg
     1861 cgcagcttgc ggtaggagtc ggtcacggtg gccaggatcg tctttccgat ccgctggtcc
     1921 tccatgtagt cgaccgtcgc ggcccagagg cggaggattt cggcccccga ctccttgatg
     1981 atcacctgcg gctcgatggt gttgcccagc gacttgctca tcttcacgcc gttctcgtcg
     2041 agcgtgaagc cgtgcgtcac gacggccttg tagggcgcgc gaccgcgggt gcccgaggac
     2101 tccagcagcg aggactggaa ccagccgcga tgctggtcgc tgccctcgag atagaggtcg
     2161 gccggccagt ggctgtcggc ccggccctcg atcgtgaagg cgtgggtcgc gcccgagtcg
     2221 aaccagacat cgaggatgtc gttgatcttc tcgaaccgct cggggtcgtg ctcgccgagg
     2281 aagtcgctgg ccgggcgcag gaaccaggcg tcggccccct ccttcgccac caggtcgacg
     2341 atgcgcttgt tgacggcctc gtcgcgcagc ggttcgccgg tatccctgtc gacgaacatg
     2401 gccagcggcg tgccccaggc gcgctggcgg ctgatcagcc agtccggacg cgtctcgacc
     2461 atgccgcgga tgcggttgcg gcccatgtcc gggtggaagg cggtggcgtc gatcgactgc
     2521 agcgccgtct cgcgcagggt gcggccgtcc gccaacggct cgtccatgcg gatgaaccac
     2581 tgcggcgtgt tgcggaagat caccggcgcc ttggacctcc agctcatcgg atagctgtgc
     2641 tccagccggc cgcgggccag caggtttccg gcctcgatca gcttttcgat caccgccggg
     2701 ttggccgggc cgaacttgcc cagcttcttg ccctcggtct cgagcacctt gaggccggcg
     2761 aacagcggca cgtggtcgta gtaggcgccg tcgggaccga cggtctcggg gatggtcgtg
     2821 tcgatgccgg ccgcctcgag ctgggccttg ctcttgagcc agatcaggta gtcgtcggcg
     2881 ccgtgccccg gcgccgtgtg cacgaagccc gtgccggcat cgtcggtgac atggtcgccg
     2941 gccagcatgg gcacggggaa gccgtaacct tcgtcgagct tcgcgagagg atggcgaagg
     3001 gtcagcttgg aacaatcgac tggcgccagg cgtgtccact tcgccacctt ggcggccttg
     3061 aagacctctt cggcgagctt gtcggccacg acgagcttgt cgcccgacgc tgcccaggga
     3121 gcgaactcca gcccctcctc cagcgcctcc acgcggtaca caccataggc gatgcgcgga
     3181 ttgtagctga ccgcccggtt ggccgggata gtccagggcg tggtggtcca gatgaccact
     3241 gatgcgtcag acgccgcacc agcgtcggcg gcgaaggctg tcagatcgat ctgcggctcg
     3301 cccggcacgg caggtggcag gtccactgcg acccaatcca ccgggaactt cacccagacc
     3361 gtcgggctgg tgtggtcatg gtactcgacc tcggcgtcgg ccagggcggt gccctcgacc
     3421 gggctccaca tcaccggctt ggagccgcga tagagctggc cgctcatcat gaacctgtgg
     3481 aactcggcga cgatcgtcgc ctcggacgtg aaatccatcg tggcgtagcg atgctcgtag
     3541 tcggccaggg tccccagccg cttgtgctcg gccatctgca cgccgatcca gccggccgcg
     3601 tattcgcggc agcgggtgcg gaactcctcg gtcgggacct cgtccttgcg acgccccttg
     3661 ccacggtatt cctcctcgat cttccactcg atcggcaggc cgtggcagtc ccagcccggc
     3721 acatagtcga cgtcgaagcc gagcgcgaag cggctgcgga cgacgaagtc cttcagcgac
     3781 ttgttcagcg agtggccgat gtggatggcg ccgttggcgt agggcgggcc gtcgtgaaac
     3841 acgaacagcg gcgcgccggc ggcctgccgg gcctttcgca cggtgttgta gaggtcctgg
     3901 tcggtccagg cctgcaggat cttcggctcc agggtcggca ggcccgcccg catcgggaag
     3961 gcggtctcgg gaaggaatac ggtttcgcgg tagtcgcgct ggggagcggc gtcgtcagcc
     4021 atgggacttt ctcggaaatc tggaaggaac gggtttcgcg acccggcgtg cgtcaccgtc
     4081 gtccggcgac gtcacgccgg gcggataatt cgcgaaatct tccgaaccga agtcatggcc
     4141 gggctgtagc agagggcgcg cctgtttgcg agcctccctc ccctttatgg ggagggtgga
     4201 cgggcaaagc cccgacgggt gggggaaccg gacgcgaccc cggctgcgtc cggcgcggct
     4261 tcgcccgtcc ccacccggac cgcctggcgg tctgtccctc ccccaggagg ggaggaaggg
     4321 gctcagctct tcttccgggc cttcggcgtc accgtgacct tcatccgcac gcccgcgcgg
     4381 acgatgtcga gcacaagggg cttgtcgatg ctggcggggc cgagcgcgcg cagcaggtcg
     4441 tccaggccgg tgatcagccg gccgtcggcg gcgacgatca ggtcgccctc gcgaatgccc
     4501 gccttctcgg ccggaccgcc ggcgtcgacc gaggcgaccc agaccgcgta gggctgggtg
     4561 acgccggctg atttggcgat ggccggcgga atcggcgtct gctgggccac cacgccgatg
     4621 gtgccgcgac gcacgtggcc gtgtttggcg atctcggcca tcacgaacga ggcggtgttg
     4681 gaggccaccg cgaaacacag gccctgggcg ccgacgatga cggcggtgtt gatgccgatc
     4741 acctcgccgt tcgaactgac cagcggcccg cccgagttgc ctggattgag cgccgcgtcg
     4801 gtctggatca ggtcctcgat cagccggccc ttctcggcgc gcaacgaccg gccgagcgcc
     4861 gagaccacgc cggtggtcac cgtggcctcg aagccgagcg gcgcgccgat ggcgatgacc
     4921 agctggcccc gccgaagctt cttggagtca ccgagccggg cgaacggcgc cttgaccggc
     4981 tgttcgacct tgagcacggc gatgtcggtg tcgacgtcgt cgccgatcgg ccgggccgtc
     5041 agggcgcggc cctcggcggt gatcaccgcc aggcgcgaga agccgccgac cacatggctg
     5101 ttggtgacga tcaggccgtc ggccgagacg aagaaccccg agccctcgcc ggcgaagctg
     5161 ccgtcggtgg tgatcggggc cacccgcacc acagccgggc cgaccgattc caccgcccgg
     5221 accaccgcgt ccgaataggc gtccagcagg tccatctcgc tgcgcacaag gcgcggctct
     5281 acccgggtat cgaggacgaa cgaggcgtcg ggcggaaagg cgaacgattc gggtttgacc
     5341 cgggtcgccg ccgggcggcg cgcgcgcgcg aaccgttcgt acttgcgtgc cttgagcatc
     5401 gggcccccac cagactggtt aacagggaac acgaggcggg ccgcggcgcc aaggggggtt
     5461 cgcccaaaca gggaatcctc cccgcttcgc ggagaggact atttctgcag aatcgcccgc
     5521 gctgtcgcct cgtcgcggcg aatctgcacg accatctcgt cgatcgaggc gaacttctct
     5581 tccggccgca ggaagccgat cagctgcgtc tcgatgacct ggccgtagag atcctcgtcg
     5641 aagtcgaaca gccagacctc cagccgcgtc tcgacctcgc cggtggtcgg gttgttgccg
     5701 atgttggcca cgccgggcag gacgcggccg tcgggcaggc gcgtgcgggt ggcgtagacg
     5761 cccagcctcg gcaccacata gtcgaccatg aacacgttgg ccgtgggaaa gcccagctgg
     5821 cggcccagct gctggccgcg gcggaccacg ccctcgatgg cgaacggccg gccgaggatg
     5881 gcggccgccg cttccgggtg gccgtcgcgc agggccgcgc gcacggcggt ggagctgtat
     5941 ttctcggccc cctccccgac cgcctcggcg accgagacgg tgaagcccag ctccgcgcca
     6001 taggccttca tcgcctcggg gctgcccgtg cggcccttgc cgaacgagat gtcgaagccg
     6061 accgccacat ggcgcacgcc caggccctcg tgcagcacct gctcgacgaa ggcgcggtcg
     6121 ctgaagttgg ccatctcgaa gtcaaaggcc agcacgtacn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggaag gccggttcgt cgggatgaaa
     6241 cagccgccgc gggtgcgggt cgaaggtgat caccgccagc ggcgcgccga tcgccctggc
     6301 cgccgtcgcc gcgccggcga tgacctgctg gtggcccagg tgcacgccgt cgaaattgcc
     6361 gagcgccacg gccgcgccgc ggtcggcggg ggccaggtgt ttccagccgt ggacgatgcg
     6421 caggctcatc aggcgggccg gcgcggaacc atgatggtgg cttcgccctc gagcacggcc
     6481 ttgccgccga cgctgcagac cgtcgccagg gtggcgcggg ccttttcagg gtccagcgcc
     6541 gtgatcgtgg cggtgaccgt caccgcgtcg ccgatcttga ccggccggcg gaaactgagg
     6601 gtctggccga gatagatggc gcccggcccg ggcagctttc cggcgatcag ggccgagatg
     6661 taggccgccg acagcatgcc gtgggcgatc cgcgtcttga acggggtcgt ggcggcgaag
     6721 gcctcgtcca ggtgcacggg gttgaagtca ccgctgacct cggcgaaggc ctggatggcc
     6781 ttctcgtcga cggtccgggt cagctcggcc gactggccga ccgacagatc ctcgaaaaac
     6841 aatccggcca tctcgccttc cccttctgat ccacaaccgc tctcatcccg gaagccgcat
     6901 agcggctatc cgggacccag attgaatcca agccgaatct gggtcccggc tcttcgctac
     6961 gccacggccg ggatgacagt ggtgagcgtc accacaccag atccgccatc gcataccgcg
     7021 ccctgacgcc ctcggcggcc agcagggcgt cgacggccgg ggccgacagc ctcccgtccg
     7081 gccccatggc ggcgtcgccg tggatgcccc tggcgacgaa caggcagtcg agcccctgct
     7141 gctcggcgcc ctgcacgtcg gtatggacgc catcgccgat gcacagcacc cgcgccatgt
     7201 cgacgtcccg gtccagcagg cgcgccgcct cggccagact cagatcgtag atcggcgcgt
     7261 agggcttgcc ggccatcagc acggggccgc ccatgtcctt gtagagatcg gccagggcgc
     7321 cgccgcagta gatcagcttg ctgccgcgct gcacgacccg gtccggattg gcgcagatca
     7381 tcgtcagacc gcgcgcggcc ggagctgtga accgctcgcg gtagtcctcc ggcgtctcga
     7441 cctcatcgtc gaacgggccg gtgcaggcga tgaaggcggc gtccgcgaca tcggcgaagc
     7501 tgaggcccag gcctgcatag agggtgttgt cacgctccgg tccgatcacc caggccgggc
     7561 ccggcgcgcg ctggccgagc agcagccggg tggcgtcgcc cgaggtgacg agcgtgctcc
     7621 aggcctcgcg cggcacgccc agggcgtcga gctgcggcac gacgtcgtgg gccggtcgcg
     7681 gcgaattgga gatcagcacc accggcccgc gctcggcccg gaaccgggcc agggcggcgc
     7741 aggcgtcggc gaagctctcg cggccattgt ggatcacgcc ccagacgtcg ctgagcagga
     7801 catcgtagtt gtcggcgaga tcggcgaggc cggcgggcag agtggtcatg gcctccggcg
     7861 gtatcggctg tgctgcggtg cgtcaatccg ctgccttcct ctccctcccg ggagaggggg
     7921 aggacaggct agctcgcgtc gtggaagatc acccccagcg tccgccgcct cccgctccgc
     7981 acctccgaaa ccccgtgccg caggatgcgc cggtgaacgc cacgcgttcc gtcatgcggc
     8041 cgctcgcgca ccgcgaagat caccgcgcag ccccgggtca gcgggacgac cgccggcgcc
     8101 gactgctggc gcggccgctg ttcgacgatc acgaactccc cgccctcgaa gtcggcgccc
     8161 ggctggtcga ggagaaacgc cgcctgcagc gggaagacat gctcgccata gaggtcctgg
     8221 tgcaggcagt tgtagtcgcc gggcccgtat ttcagcagca ggggcgtggg ccggagttgg
     8281 ccggccgcgt ggcagcgccc gagcatcccg gccagcgtcg gggggaaccg ccgcgcctca
     8341 cccagccgct ccgcccagcc gttggcgatc gccgccagcg ggggatacag gccctcccgc
     8401 aggacctgca cggcgtccgg cagcggatag ctgaaatagc ggtattcgcc ctggccgaag
     8461 ccgtgccggg ccatgaccac ccggctgcgg aagccggcct cctgctcata cagcccggcc
     8521 agggccgcgc aggcgtcgtc gtccagcagc cggcccgtga cggcccagcc cctcgcgtcg
     8581 aggtcggcgg cgatgcgctt ccagtcgagg acgtcgggtt tcacgcggcg gcgtccggtt
     8641 tgcagcgcct gcaggcacgg tacccgtcgg cgcgagccgc cccggcggtc gcatagaagg
     8701 ccacattgcg ccgcaggggc cggccgctgc agctcggccg gcaatagacg ccggtggtct
     8761 tcaccgcgaa caggaagacg ccgtcggcat cccggtcgcg gcgctgcatg gccgcccagc
     8821 gcgcgtcgtc attgtcatag gtcatggtca cgctcctcgt cgggaggcga ccctgcgcgc
     8881 ggggaggagc cggcgcattc cgatgcttgc tatcgcgtgg aaaaacccgt cgggcctacc
     8941 agcccgacgc ccggcgcagg gtggtgcgca tgaagtcggc cggcggatcg gcctcggcca
     9001 ggatggcgtc gcggatggcg cgcggctcgc cgaacaggtg gccctggccg aaggccatgt
     9061 cgagctcgag gatgtcgacg atctgacgct cgctctcgac cttctcgatg atcacctcga
     9121 cgccatagcg gcgggtaagc cgggcgaagt cggcggcgtg caggtcgggc agcgagcgaa
     9181 tcaccagccg gccctcgctc tcctcgagct ggtcgagcag catctgggcg ccgaccttga
     9241 tgaacttgat gtccgaccgc gccaggtcct ggaagtcgag atccaggtcg acgaccttgt
     9301 cgaggctgaa gcggaagccc aggtcggcca gccgcgccat gtgacgggcc tcgaccgcgc
     9361 cgcgtcgttc gaacgccgcc tggcccagtt cgaagatcag ggccccggcc atgtccttgt
     9421 tggccgacag aaagtcgagg aactgcggga agaaggtctc gtcggacagg ctggccagcg
     9481 agatgttgca gaagatgccg accttgcggt cctgcctggc cagccggcga acgatctgca
     9541 cgcagcggaa cagcagcagg ttgtcgatcg ccgccacgag accctcgggc tcggcgaccg
     9601 aaaggtattc ggcgggcatc atcacgcggc cggtctcgtc gcgcagccgc gagaaacttt
     9661 cgtagaacac ggtccggcgt tgcggcagcg acacgaccgg ctgcaggtag aggtcgaccc
     9721 ggttgccggc cagggcgtcc ttgacggtgt ccagcagggc ggctgactgg gggcctcggc
     9781 ccagggcgtg cggcgcggcg gcggcgaccc ggctggagag ctgggcctcc atcttctcgg
     9841 ccatcttctg gacggcgtcc tcgagcgaat ggacctcgct ggtgagttcc tcggaccggc
     9901 gcagggcgtc ggcggtcacg ctctcgacca cctcgttcag gcggccgtcg atcttctcga
     9961 tctggcgggc gaggatcagg tgggcctctc ggatattgtc cagctcgccg gtgacgcggg
    10021 cggtgttgat cgccccgccg atcaggccat ggaaggcgaa ggccagaccc agaccgccga
    10081 tcagcgcggc gatgcccgca cccgccccgt agccgtagcg ccacatgagg aacgcgaccg
    10141 cgagggtgat acagaaatag gcgccgctca gcagcgcaag ggtgagcctt cgcatcgtcc
    10201 cgcctgcacg aatcgtccgg agtatccgct gcgccgcatc atggtgtcca aggtattaac
    10261 gccgcactgc cgtccacagg cgattctgcg ccatcgacag gaccggtccg gaatgataat
    10321 cattctcaaa gaacctcggg gcgtccatgt ccgaagccgc tgtccttgaa ccacaatccc
    10381 cgccgacgct gaccagcgcc gacatcatcg atgtcggccc gctgagcgcg gccggcgtcg
    10441 gcggccgtgg cgtgatcgtc cgcgtctccg ccttcgaggg cgccctgccg gcggccgagc
    10501 tggagcggcg gctgctggaa ctcggcttcg tcgagggcgc cgtggtcgag atcctgcacg
    10561 agggcgcctt cggccgcgac ccgatggcgc tgcggctgga cgacatgcgc gtggcgatcc
    10621 ggcgccgcga ggccgccgcc atccaggtcc gcttcgactc aagggccggc gcttgaccca
    10681 cgcgtccctg gcccccgcgc gtgtggcgct ggtgggtaac ccgaacagcg gcaagaccgc
    10741 cctgttcaac gccttgaccg gcagccgcca gaaggtggcc aactacgccg gcgtgaccgt
    10801 cgaacggaag gaaggcgtgc tgaccacccc ggccggccgg gtcatccagg tgctggacct
    10861 gccgggcacc tattccctgc gggcccgcag tccggacgag gccgtgaccc gcgacgcggt
    10921 gctgggccgg ctggccggcg aggttccgcc cgacgtgatc atctgcgtcg ccgacgcgac
    10981 caacctgcgg ctggtgctgc ggctggcgct ggagctcagg caggtcggcc ggccgttcgt
    11041 gctggccctc aacatgtacg acatcgccca gcgtcagggg cttcgcatcg acctggacgg
    11101 cctgtcgcgc gagatcggcg ccccgatcgt caccaccgtc gccacgcgca agcgcggcat
    11161 cgaggaactg gtggcccggg ccgaggccct ggcggacacc atgccggtcg gcgagaacgt
    11221 ctggcacgag ccgtcggcgg ccgagattcg ccatgcccac gccgaggccc agcgtatcct
    11281 gaaggcccac gtccggccgc ccgagcggcc cgacacctgg accagtcgca tcgacggcgt
    11341 gctgctgcat ccggtcgccg gcctgctgat cctgtcggcg atcatgttcg tgatgttcca
    11401 ggcggtgttc gcctgggcga cgcccgtggc cgatctgatc gaggccggct tcacctggct
    11461 tggcggggcg gttgccacgg tcacgcccga gggcctgctg cgcagcctgt tggttgacgg
    11521 cctgatcgcc ggggtcggca gcgtgctggt gttcctgccg cagatcctga ccgtgttctt
    11581 cttcatcctg ctgctcgagg acagcggcta catggcccgg gccgccttcc tgatggaccg
    11641 catcatgggc gcggcgggcc tgcacgggcg ggccttcatc ccgctgctgt cgagccacgc
    11701 ctgcgccatt ccgggcgtca tggccgcgcg ggtcatcgac cagaagcacg accggctgac
    11761 gaccatcctg atcgcgccgc tgatgacctg ttcggcgcgc attcccgtct acaccctgat
    11821 catcggcgcc ctgattcccc ggacgacggt ctggggcttc ctgaacctgc aggggctggt
    11881 gatgttcggc ctctacgccg ccggcatcct gtcgggcctg atcgtcgcca cggtgatccg
    11941 caccctgttc tggaggagcg cgtccgagcc gttcatgatg gagctgccga cctatcgctg
    12001 gcctgatcct gagaacgtcg cgcgcaattt gatgcagcgg gcgatgatct tcatcaaccg
    12061 cgccggccgg atcatcctgc cgctgatggt gctggtctgg gtgctgtcga cctttcccta
    12121 tccgccggcg ggcgcgacgg gcccggccat cgactacagc ttcgccggca tgatcggcca
    12181 cgtcatccag ccgctgctcg cgccgatcgg cttcaactgg cagatggccg tggcgcttgt
    12241 gcccggcatg gccgcccggg aggtcgcggt ggcggcgctg ggcacggtct acgccgtggg
    12301 cggcgccgat ggcgcgggcc aggcgaccct ggcctcgatc ctccacgccc agtggagcct
    12361 ggccaccggc ctgtcgttcc tggcctggta cgtcttcgcg ccacagtgcg tggcgacgct
    12421 gggcgtggtc aagcgcgaga ccaacggctg gctctggccg acggtgatgt tcggctacat
    12481 gttcgccctg gcctacctgg cctcgctggc gacctactgg ggcgccgtgg cgctgggctg
    12541 gggctgagcc ctaccgcgtc ggaacgggcg tttcgccgcg gtagtcgtag aagccgcggc
    12601 cgaccttctt gcccagccag ccggcctcga cgtacttcac cagcagcgga caggggcggt
    12661 acttgctgtc cgccagcccc tcgtgcagca cgttcatgat cgacaggacg gtgtccaggc
    12721 cgatgaagtc gcccagctcg agcggaccca tcgggtggtt ggcgccgagc ttcatcgccg
    12781 tgtcgatcga ctccaccgtg ccgacgccct cgtacagggt gtagatcgcc tcgttgatca
    12841 tcggcaccag cacgcggttg acgatgaagg cggggaagtc ct
//
LOCUS       DAORNX010000025        12557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3789_length_12557_cov_8.300032, whole genome shotgun sequence.
ACCESSION   DAORNX010000025 DAORNX010000000
VERSION     DAORNX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_12557_cov_8.300032"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLO65_02070"
     CDS             complement(<1..625)
                     /locus_tag="PLO65_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRD27065.1"
                     /translation="MDGVVRAGVIATMFWGAVGMLVGVVIAAQLSWPNLLYFPDFGWL
                     NFGRLRPLHTSGVIFAFGGNALICTSFYVVQRTCRARLFGGVLPWFVFWGYQLFIVLA
                     ATGYLTGITQSREYAEPEWLTDLWLTVVWVAYLFTFLGTIWKRKEPHIYVANWFYLAF
                     IVTVALLHIVNNLALPVGFLNHRSYSLFSGVQDALTQWWYGHNAVGFF"
     gene            971..1222
                     /locus_tag="PLO65_02075"
     CDS             971..1222
                     /locus_tag="PLO65_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27066.1"
                     /translation="MHVRALAHTPQLELITLHSTLGALALWLGVLIREWPQLTAYGVF
                     CGEPQTLFGHCPLCFPAAALTVIAMAGMTMLARRGARRA"
     gene            complement(1219..1668)
                     /locus_tag="PLO65_02080"
     CDS             complement(1219..1668)
                     /locus_tag="PLO65_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27067.1"
                     /translation="MDKRLDKVVVTRLSAGEVDEAFGLARLAFPTLTPGRWRATARRW
                     TAHPTGAAGALVARDSAGRLIGLAPFTVQTNLQAGRSLWVERVASFALLDGRPVIEAL
                     ADGLRDVARTLGCRGLTVQIETGDRALLAALGRGCDSARSTLLNATV"
     gene            1749..2501
                     /locus_tag="PLO65_02085"
     CDS             1749..2501
                     /locus_tag="PLO65_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27068.1"
                     /translation="MTGTVESPVCASDGRIDPCSLCSARKLSVCGVLADEGLRRLSGI
                     SDHQDFTPGEILVREGDPATNLFNITTGSVRVYKLLPDGRRQIVGFLFPGDFLGLATG
                     DRYAFSAEALTSGSACRFHKKVYRRMLTELPDLEAALLDRASHELQAAHNQMLLLGRK
                     TAVERLASFLSDLAARDRRAGGPGRVIQLPMTRAEIADYLGLTTETVSRVTSRLKTRG
                     VIRLLSLHSLSIEKPAELAELAGLDAGDGEAL"
     gene            complement(2498..3145)
                     /locus_tag="PLO65_02090"
     CDS             complement(2498..3145)
                     /locus_tag="PLO65_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HRD27069.1"
                     /translation="MKTVIATAVIAAALAAAGAASAQTFEPKAAGTVLVDFRATSVAP
                     DESGSILTSGGVDTGLDVKVNNDTIPSLGLSYFITDKVAVELILGTSKHEISATTAPT
                     ATPVHDTWVLPPVLSLQYHPLPAARFSPYVGAGVNYMIFYNGKDRNGFKVDLDNGFGY
                     SLQAGVDIAVQGPWSVNVDVKKVFFDTEASINNGALRSDVTLDPLVVSVGLGRKF"
     gene            complement(3363..4472)
                     /locus_tag="PLO65_02095"
     CDS             complement(3363..4472)
                     /locus_tag="PLO65_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRD27070.1"
                     /translation="MLNVKLVDSPPQDVREFNDELTHQLTSEDVEVIREIFRTPLTGS
                     YNWDYQDANARIRKLYELGKRFNWDAQMDVDWDAPFSRDEMPNDPTMNAFAGHPKYEA
                     LSPQQKLDFAWRSHAQTLSQFLHGEQGAMLVASQLVSCAPTYDAKLYASSQTFDEARH
                     VEVFHKYLTERCKIVYPINPNLKLLLDKVLTDERWDLKFIGMQVLIEGLALAAFQSMT
                     QSTRDPLLRQILELVMRDEGRHVAFGVNYLEDWIRALPEHEIEERAQFAYEACVIMRE
                     RLFSVDVPQEYGFTLEEAREINHKAQAGGAFRDFLFARMIPNLKRIGLLTESVRPKFE
                     ALGVLRFEDEVHDGLIDWATLEKPLPVTLRQMAAE"
     gene            complement(4570..4695)
                     /locus_tag="PLO65_02100"
     CDS             complement(4570..4695)
                     /locus_tag="PLO65_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27071.1"
                     /translation="MLETLTALPTVHQAYLGMVIVGFCAFGLTLGVVSTLVNLKN"
     gene            complement(4762..5169)
                     /locus_tag="PLO65_02105"
     CDS             complement(4762..5169)
                     /locus_tag="PLO65_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27072.1"
                     /translation="MRFEGSTVAAGRPTAIVVDDDEDLLNALKFSLELDDVQVVGHRS
                     AETVNASALPADNACLVLDYRLPATNGLDLLTRLREEGVTLPAILVTSHASAIVRSLA
                     RAAGATLVEKPLLGDTLLTAIRQVLRGAGENQK"
     gene            complement(5224..5829)
                     /gene="fixJ"
                     /locus_tag="PLO65_02110"
     CDS             complement(5224..5829)
                     /gene="fixJ"
                     /locus_tag="PLO65_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HRD27073.1"
                     /translation="MTEALVHIIDDDEAMRDSLAFLLDTAGFSVMASASARAFLKTIP
                     DPRLGCIVTDIRMPDMTGLELIKALRADGHDYPVIVITGHGDVPLAVEAMKSGVIDFI
                     EKPFDDETLLDAIRIAFRRQTADGGVSPERQAIIARLEELSPREREVLSGVVAGKPNK
                     VIAYDLGISPRTVEVYRANLMTKMAAGSLSELVRMALVAGY"
     gene            complement(5822..7003)
                     /locus_tag="PLO65_02115"
     CDS             complement(5822..7003)
                     /locus_tag="PLO65_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRD27074.1"
                     /translation="MTDFRKPIEDDVEPEHALEALREREAHLRSILDTVPDAMVVIDI
                     HGLIQSFSATAERLFGWSAADVIGRNVSMLMPQPYRDAHDGYLDRYLTTGERRIIGIG
                     RVVVGERRDGSTFPMELAVGEMKSADRRYFTGFVRDLTERQETERRLQDLQSELVHVS
                     RLTAMGEMASALAHELNQPLSAAANYLKGSRRLLESETPDLPRLRDAMEKAADQTLRA
                     GQIIRRLRDFVARGESEPRIESLPKAIEEAGVLALIGAAERGVRVRNRIDSRVDLVLA
                     DKVQIQQVLVNLMRNAIEAMQDSERRELTVAAAPVADGMIEVMVADTGSGIHPDVADR
                     LFQPFFTTKSQGMGVGLSISRTIVEAHGGRIWVETNEKGGATFRFTLRAVGPEDLGDD
                     D"
     gene            complement(7061..7720)
                     /locus_tag="PLO65_02120"
     CDS             complement(7061..7720)
                     /locus_tag="PLO65_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27075.1"
                     /translation="MIASNASQAFAAAAHSAGSALALDAIDRLGVRESFGKDEEIYGQ
                     AERVERIYRVISGAVRTTRLLSDGRRQVGEFYFPGDVFGLETGPDHRYSAEALCDSVV
                     LSVKRSALRASDAAAEFDRMIWDATARELDRTQDHVLMLGRKTACERVASFLRGVVER
                     TGGLGDLPMSRQDMADYLGLTIETVSRMLSQLQSTSVVEFDALRHFRVRNPQALARLA
                     A"
     gene            complement(7828..>8448)
                     /locus_tag="PLO65_02125"
     CDS             complement(7828..>8448)
                     /locus_tag="PLO65_02125"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRD27076.1"
                     /translation="RHRHRPAAGDLAAMANVLVINGHPDADPRRLNAALARSCGDGAR
                     AGGHAVRTLDLGRLDFPLVASRQAFESTPPPEVIVQAQEAIVWARHIILVFPLWLGGP
                     PALVKAFLEQTFRYGFALAKPGSPKGGGLLGGRSVRIIVTMGMPAFLYRLAFGAFGVR
                     AIERGVFRLAGMGPVRHTLLGGVETATDAVRAGWLDNVRRLAAVPA"
     assembly_gap    8449..8458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8654..>10515
                     /locus_tag="PLO65_02130"
     CDS             8654..>10515
                     /locus_tag="PLO65_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27077.1"
                     /translation="MLTAAYAIAVYSGVMWSRSVENSTAFWAANGVLAAGLLLLRPRV
                     AIAFALVCLGVNFGVNVVGRLPLELNLAFTSLNIAFSAAVAMLVRTFCGAALDLGRLR
                     RFLPFLAIVACAALVEGMIGSSMISTSPEQFLTIWRRWMACDGVGMVLGLPATLMMMR
                     GVNPLHAGPADARERVALLAVLGLGTYAAFAVAAAPVFLLIFPALLFAAFRLGSGWAF
                     LAVWICGQAATIQTLAGRGPIAVFDGPPPFIELGLLQLFLLSILLTASLATSALAERM
                     RAEQRLRRKAAATAAARARIDQVTATRERFLAVISHEIRTPLNGIVGFGEALAKRADL
                     DPEARHQVEMIGRSTEALMGIVDDILDYARIESGRLDIKPEAASVEQTLARAVEAAAP
                     LAIAKGLTLTRTGVALEDALHRVDARRLQQVFGHLIDNAVKFTAAGGVEVRLECAPAG
                     DDEKLTVTVSDSGQGVPEHQRRHLFRPFAQLDPSLSRRHGGAGLGLSICRAIVDLMDG
                     EISYRPGPAGGSVFSMSVTLPRAGVATSQDEGLGERAPRVLVVDDHPMNREVARLFLD
                     AFGCEVSECEDGGAAVEAVAGGAFDVILMDVRMPGVDGLSATRMIRELGGPASLT"
     assembly_gap    10516..10573
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10694..11587)
                     /locus_tag="PLO65_02135"
     CDS             complement(10694..11587)
                     /locus_tag="PLO65_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD27078.1"
                     /translation="MEMQQVRYFLALADTLNFTRAAERSGVTQPALTRAIQQLEAELG
                     GPLFHREGRRTHLSELGRTMLPYFEGILAQAEAARSEARSLKALDRATLHIGVMCTVG
                     PAIIADLVVAFRGANPGVDLRVSDMAAAELAGRLEAGDLDLALFGLPDGVDDRFHALP
                     LFRERFVVAVPRDHRFAAQNVVRAADLHDENYVSRASCEYFDHAGRQFRGRGVVMRKV
                     FSSERDDWVQGMIKAGLGLGFIPEFSVTDPALVIRKLIDPEFERTIMLVTVRGRPHAP
                     AVGAFVRQARAFPWPEPRDLA"
     gene            11708..12142
                     /locus_tag="PLO65_02140"
     CDS             11708..12142
                     /locus_tag="PLO65_02140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD27079.1"
                     /translation="MADDDSTGVEDLNRTLGLVRRIEMNPEGRAVIEYEAGPHMCHSG
                     GVVQGGFVTGWIDAAMAHAAIAMTGPGVVPMSLELKVSFFAPARPGRVIAEAWVERRG
                     RTTCFFEGRLLDPAGKVLAKASSTLQLVDRAKVEAASRAAAT"
     gene            12239..>12557
                     /locus_tag="PLO65_02145"
     CDS             12239..>12557
                     /locus_tag="PLO65_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD27080.1"
                     /translation="MHYAELNEAWAELTAPGAPFEIAEIEVRGARIRSFRNAPPSVRE
                     VWLSTLPFGERDYLVYEDERITYGEAHRQVASIANWMMAHGVKPGDRVAIAMRNYPEW
                     MLIY"
BASE COUNT         1996 a         4216 c         4309 g         1968 t
ORIGIN      
        1 ggaagaagcc gaccgcgttg tggccgtacc accactgggt cagggcgtcc tgcacgccgg
       61 agaacaggct gtagctgcga tggttcagga agccgaccgg cagggccagg ttgttgacga
      121 tgtgcagcag cgcgaccgtg acgatgaagg cgagatagaa ccagttggcc acgtagatgt
      181 gcggctcctt gcgcttccag atcgtgccca ggaaggtgaa caggtaggcc acccagacca
      241 cggtcagcca caggtcggtg agccactccg gttcggcgta ctcgcgcgac tgggtgatgc
      301 ccgtcaggta gcccgtggcg gccaggacga tgaacagctg gtagccccag aacacgaacc
      361 acggcaggac gccgccgaac aggcgcgccc gacaggtccg ctggaccaca tagaagctgg
      421 tgcagatcag ggcgttgccg ccgaaggcga agatcacgcc ggaggtatgc agcggcctta
      481 gccggccgaa gttcagccag ccgaagtcgg gaaagtagag caggttgggc caggacagct
      541 gggcggcgat gaccaccccc accagcatgc ccaccgcgcc ccagaacatg gtcgcgatca
      601 caccggcccg gacgacgccg tccatgtatt tgctctggtc gttgttcagc aggccgccgc
      661 tgatcgccgc gttgagcgcg aagccgccga ccacggcggc ggcgacgaag atgtaggcct
      721 ggaaacggaa aagcggatcg tccgtcgccg cagcggccag ggcgaacagc agcccgccaa
      781 gaaccgtgaa ggtcagtagg atcggtccgt ccagggaacc gccgcccggt ttcgatacct
      841 gtttcatata tgtgccccaa gctgtcttgc ggttcagcta gggccgcccg ggtggtgctg
      901 cgttgatctg catcaaggct ctcgcggcgc tcgcggggaa aagaatgcag cgcaacatcg
      961 ggagtcgaag atgcacgtcc gcgcgctggc ccacacgcct cagcttgagc tgatcaccct
     1021 gcacagcacc ctcggggccc tggcgctctg gctgggcgtt ctgatccggg aatggccgca
     1081 actgacggcc tacggcgtgt tctgcggcga gccgcagacc ctgtttggtc actgtccgct
     1141 gtgcttcccc gcggcggccc tgacggtcat cgcgatggcc ggcatgacca tgctggcccg
     1201 ccgcggcgcc cggcgcgcct agaccgtggc gttgagaagg gtggatcggg cgctgtcaca
     1261 gccgcgacca agcgccgcaa gcagcgcgcg gtcgcccgtc tcgatctgca ccgtcaggcc
     1321 gcggcagccg agcgtccggg cgacatcccg caggccgtcg gccagcgcct caatgaccgg
     1381 acggccgtca agcaacgcga acgaggccac ccgctcgacc cagaggctgc ggccggcctg
     1441 caggttggtc tgcaccgtga acggcgccag gccgatcagc cggcccgcgc tgtcgcgggc
     1501 caccagggcg ccggccgctc cggtgggatg ggccgtccag cggcgcgcgg tggcccgcca
     1561 gcgcccgggc gtcagagtcg gaaaggccag ccgggccaat ccgaacgcct cgtcgacctc
     1621 tccggccgaa agccgggtga cgaccacctt gtccaaacgc ttgtccatct tgtgtttcag
     1681 gctcgcagga cgctccgtca ggatcgttga tctgcgtcaa gactgccaat cctctgggag
     1741 gcccctccgt gaccggaacc gttgagtccc ctgtctgcgc cagcgacggc cggatcgacc
     1801 cgtgcagcct gtgcagcgcg cgcaagctga gcgtctgcgg cgtgctggcc gacgagggcc
     1861 tgcggcggct gtccggaatc tcggaccatc aggacttcac gcccggcgag atcctcgtcc
     1921 gcgagggcga tccggcgacc aacctgttca acatcaccac cggttcggtg cgggtctaca
     1981 agctgctgcc cgacgggcgc cggcagatcg tggggttcct gtttcccggc gacttcctgg
     2041 gcctggccac gggcgatcgc tacgccttct cggccgaggc cctcaccagc gggtccgcct
     2101 gccggttcca caagaaggtc tatcggcgga tgttgaccga gctgccggat ctcgaggccg
     2161 cgctgctgga ccgcgcgtct cacgagctgc aggccgcgca caaccagatg ctgctgctgg
     2221 gacgcaagac cgccgtcgag cggctggcct cgttcctgag cgacctggcc gcccgggatc
     2281 gccgcgccgg tggtccggga cgggtgattc agctgcccat gacccgggcg gagatcgccg
     2341 actatctggg tctgacgacc gagacggtga gccgggtcac gtcgcgactg aagacgcgcg
     2401 gcgtgatccg gctgctgtcg cttcacagcc tcagcatcga gaaaccggcc gagctcgcgg
     2461 agctggccgg cctcgacgcc ggggatggcg aagccctcta gaacttgcgt cccaggccga
     2521 ccgacacgac cagcgggtcc agcgtcacgt ccgagcgcag cgcgccgttg ttgatgctgg
     2581 cctcggtgtc gaagaacacc ttcttgacgt cgacattgac cgaccagggc ccctgcacgg
     2641 cgatgtccac gccggcctgc agcgagtagc cgaagccgtt gtcgagatcg accttgaagc
     2701 cgttccggtc cttgccgttg tagaagatca tgtagttgac gccggcgccg acgtacgggc
     2761 tgaagcgcgc ggccggcagc gggtggtact gcagcgacag gaccggcggc agcacccagg
     2821 tgtcgtgcac cggcgtggcc gtcggcgcgg tggtggccga gatttcatgc ttcgacgtgc
     2881 cgaggatcag ttcgacggcc accttgtcgg tgatgaagta ggacagcccc aggctgggaa
     2941 tggtgtcgtt gttgaccttg acgtcgagcc cggtgtccac gccgccgctc gtcaggatcg
     3001 aacccgactc gtcaggggcc accgaggtgg cgcggaagtc gaccagcacg gtccccgcgg
     3061 ccttgggttc gaaggtctgg gcggaagcgg cgccggcggc cgccagggcg gcagcgatga
     3121 ccgcagtggc gatcacggtt ttcatcttga tatccccttg gttttgggcc ggggctgttg
     3181 tcgctccgga cagctcatca atatcggatc gcgcaattca ggcagtgacg tggatcaatt
     3241 ctcgcggcca ctccatcgat gacttgaccc ggcatcgccg ggcgccgcgc ccaaaaaaga
     3301 aggcccccgg gccggaggag ccgggggcct gaaggtggag gagatggaat gccggccggc
     3361 ggctattcgg cggccatctg gcggagcgtg accggcaggg gcttctcgag cgtcgcccag
     3421 tcgatcaggc catcatggac ctcgtcctcg aaccgcagca cgccgagggc ctcgaacttg
     3481 ggccgcaccg actcggtcag caggccgatc cgcttgaggt tgggaatcat ccgcgcgaac
     3541 aggaagtcgc ggaacgcgcc gccggcctgg gccttgtggt tgatttcgcg ggcttcctcg
     3601 agcgtgaagc cgtactcctg cggcacgtcc acgctgaaca gccgctcgcg catgatcacg
     3661 caggcctcgt aggcgaactg ggcgcgctcc tcgatctcgt gctcgggcaa ggcccggatc
     3721 cagtcctcga ggtagttcac cccgaacgcc acgtggcgcc cttcgtcgcg catcaccagt
     3781 tcgagaatct ggcgcagcag cggatcgcgg gtgctctggg tcatcgactg gaaggccgcc
     3841 agggccaggc cctcgatcag cacctgcatg ccgatgaact tcaggtccca gcgctcgtcg
     3901 gtcaggacct tgtcgagcag cagcttcagg ttcgggttga tcggatagac gatcttgcac
     3961 cgctcggtca ggtatttgtg gaacacctcc acatgccggg cttcatcgaa ggtctgcgac
     4021 gaggcgtaga gcttggcgtc ataggtcggg gcgcagctga ccagctggct ggcgaccagc
     4081 atcgccccct gctcgccgtg gaggaactgc gacagcgtct gggcgtggct gcgccaggcg
     4141 aagtcgagct tctgctgcgg cgacagggcc tcgtacttcg gatggccggc gaaggcgttc
     4201 atcgtcgggt cgttgggcat ctcgtcgcgc gagaacggcg cgtcccagtc gacgtccatc
     4261 tgggcgtccc agttgaaccg cttgccgagc tcgtagagct tgcgaatgcg ggcgttggcg
     4321 tcctggtagt cccagttgta ggacccggtc agcggcgtac ggaagatctc gcggatcact
     4381 tccacgtcct ccgaggtgag ctggtgggtc agttcgtcat tgaattcccg cacgtcctgg
     4441 ggcggcgagt cgaccagctt gacgttcagc atttcctgtc tcccgggctt gtcgcgtcaa
     4501 agatgacgca ggcgtcattt tttggttatg aaaaacgccg gcgggcggac ccgaccggcg
     4561 tcagcggctt cagttcttca ggttgaccag cgtggacacc acgccgagcg tcaggccaaa
     4621 ggcgcagaag ccgacgatga ccatgcccag ataggcctgg tgaacagtcg gcagggccgt
     4681 cagggtctca agcatcgcta tgctccatgc agatcacagc gaattaggtc ccggcgcggg
     4741 gctcccgatc attgacctgg atcatttctg gttctcgccg gcgccgcgaa ggacctggcg
     4801 gatcgccgtc agcagggtgt cgccgagcag cggtttttcc accagggtcg cccccgccgc
     4861 ccgcgccagc gagcggacga tggcgctggc gtggctcgtg accaggatcg ccggcagggt
     4921 gacgccctcc tcccgaagcc gggtgaggag gtcgaggccg ttcgtcgcgg ggaggcggta
     4981 gtcgagcacc agacaggcgt tgtccgcggg aagggccgag gcgttgacgg tttcggccga
     5041 ccggtgaccg acgacctgga cgtcgtccag ctcgagagag aacttgagcg cattgagcag
     5101 atcctcgtcg tcgtcaacga cgatcgcggt cggacggccg gccgccacgg tgctcccctc
     5161 gaacctcatc gagtgacttt gagcgaacga aggaacgccg ccttgatctg gctcaaagac
     5221 agatcagtag ccggcgacca gcgccatgcg aaccagctcg gacaggcttc cggccgccat
     5281 cttggtcatc aggttcgccc ggtagacctc gaccgtccgc gggctgatcc ccagatcgta
     5341 ggcgatcacc ttgttgggct tgccggcgac gaccccggag aggacctcac gctcccgcgg
     5401 ggagagctcc tccaggcggg cgatgatcgc ctggcgttcc gggctgacgc ctccgtcggc
     5461 cgtctggcga cggaaggcga tgcggatcgc gtcgagcagg gtctcatcgt caaacggctt
     5521 ctcgatgaag tcgataacgc cggacttcat cgcctcgacg gccagcggca catcgccatg
     5581 cccggtgatg acgatcaccg gatagtcgtg gccatcggcg cggagcgcct tgatcagctc
     5641 caggcctgtc atgtccggca tgcggatgtc ggtgacgatg caaccgagcc ggggatcggg
     5701 aatggtcttg agaaaagccc tcgccgacgc gctcgccatc acgctgaatc cggcggtgtc
     5761 gaggaggaac gccagggagt cccgcatcgc ctcgtcatcg tcgatgatgt gaacgagcgc
     5821 ctcagtcatc gtcgcccaga tcctccggtc ccacggccct cagggtgaac cggaaggtcg
     5881 ccccgccctt ctcgttggtc tccacccaga tccggccgcc gtgcgcctcg acaatcgtgc
     5941 gtgagatgga cagacccacg cccatgccct gcgatttggt ggtgaagaac ggctgaaaca
     6001 agcggtcggc cacgtcagga tgaattccgg agcccgtgtc ggccaccatg acctcgatca
     6061 tgccatcggc gaccggggcc gccgccaccg tcagttcgcg gcgttcggag tcctgcatcg
     6121 cctcgatcgc attgcgcatc agattgacca gcacctgctg gatctggacc ttgtcggcga
     6181 gaacgaggtc gacgcggctg tcgatccggt tgcgcaccct gacgccccgc tcggccgccc
     6241 cgatgagggc cagaaccccc gcctcctcga tcgccttggg cagactctcg atccggggct
     6301 cggactcgcc gcgggccacg aagtcccgca gccgccggat gatctggccg gcccgcagcg
     6361 tctggtcggc ggccttctcc atggcgtcgc gcagccgtgg caggtccggc gtttccgact
     6421 ccagcagccg gcgggaaccc ttcagatagt tggccgcggc cgacagcggc tggttcagct
     6481 catgcgccag ggccgaggcc atttcgccca tggccgtcag gcgagacacg tgcaccagct
     6541 ccgactgcag gtcctgcaga cgtcgttcgg tctcctgtcg ctcggtcagg tcgcggacga
     6601 agccggtgaa gtagcggcga tcggcggact tcatctcgcc cacggccagt tccatcggga
     6661 aggtcgagcc gtcgcggcgt tcgccgacca ccacccgtcc aatgccgatg attcgccgct
     6721 cgccggtcgt cagatagcga tcgagatagc cgtcatgggc atcgcggtaa ggttgcggca
     6781 tcagcatgct gacattgcgg ccaatgacat cggccgcgct ccagccgaac agccgctcgg
     6841 ccgtcgcgct gaacgactgg atcagcccgt ggatgtcgat cacgaccatc gcatcgggca
     6901 cggtgtcgag gatggagcgc agatgcgctt cacgctcccg aagcgcctcc agggcgtgct
     6961 ccggctcgac gtcgtcctct atgggttttc tgaaatcagt catgctcgcc ccgagcgact
     7021 atacccatcc gggcatgtcg gtcgaggcgc gcattttgtc tcaggcggcg aggcgcgcga
     7081 gcgcctgcgg gttgcggacc cggaaatgac gcagggcgtc gaactcgacg accgaggtcg
     7141 actgcagctg gctcagcatg cgcgacaccg tctcgatggt caggcccaga tagtcggcca
     7201 tgtcctggcg gctcatcggc aggtcgccca ggccgccggt ccgctcgacc acgccgcgca
     7261 ggaagctggc cacgcgttcg caggcggtct tgcggccgag catcagcaca tggtcctgcg
     7321 tccggtccag ttcgcgggcc gtggcgtccc agatcatccg gtcgaattca gcggcggcgt
     7381 cgctggcccg cagggccgaa cgcttcaccg acaggacgac gctgtcgcac agggcctcgg
     7441 ccgagtagcg atggtcggga ccggtctcca ggccgaagac gtcgcccggg aagtagaact
     7501 cgccgacctg gcgacgcccg tcgctgagca gccgggtcgt gcgcaccgcg ccgctgatca
     7561 cgcggtagat ccgttcgacg cgctcggcct ggccgtagat ctcctcgtcc ttgccgaacg
     7621 actcgcggac gcccagccgg tcgatggcgt cgagcgcgag ggcggatccg gcggagtgcg
     7681 cggcggcggc gaaagcctga gaagcgttgg aggcgatcat gtcaattccc cttgcctgat
     7741 gtgcaaaggg gtaggccgac gagcggcgcg ccgaaattca gggcgtgtgc ttaagggagt
     7801 ctacgtaggc ctgcggagag cgtgaaatca ggccgggacg gcggccagcc gcctgacgtt
     7861 atcgagccag ccggcccgga ccgcgtcggt cgccgtctcg acgccgccca gaagggtgtg
     7921 ccggaccggt cccatgcccg ccagccggaa caccccgcgc tcgatggccc tgacgccgaa
     7981 cgcgccgaag gcgagacgat agaggaacgc cggcatgccc atggtgacga tgatcctgac
     8041 cgagcgaccg ccgagaagac caccgccctt cggcgacccc ggcttcgcca gggcgaaccc
     8101 gtagcgaaac gtctgttcga gaaaggcctt gaccagcgca ggggggccgc cgagccacag
     8161 cggaaacacc aggatgatgt ggcgggccca gacgatggcc tcctgggcct ggacgatcac
     8221 ctcgggcggc ggagtcgact cgaacgcctg gcggctggcc accagcggga aatcgagccg
     8281 gcccaggtcg agcgtccgaa ccgcgtggcc acccgcccgc gcgccatcgc cgcagctccg
     8341 ggccagggcc gcgttcagcc gccggggatc ggcgtcgggg tggccgttga tgaccaggac
     8401 attcgccatg gccgccagat cgcccgcggc gggccggtga cggtgacgnn nnnnnnnnga
     8461 tcgcgcacgg caggccggcc cgccatgacg ttgatcaaaa cccggccggt ggagggcggg
     8521 acacgattca ccgctttcca acacttccat gaccctatgg agcggaacga cagggccgca
     8581 catgaccgtg tttggaattc gctggcggtg gcgccgcccc gcccaacggc aacgcgggtg
     8641 ggacgcggcc ctggtgctca cggccgccta cgccatcgcc gtctattccg gggtgatgtg
     8701 gagccggagc gtcgaaaatt ccacggcctt ctgggccgcc aatggcgtgc tggccgccgg
     8761 cctgctcctg ctgcgccccc gggtcgccat cgccttcgcc ctggtctgtc tgggggtgaa
     8821 cttcggcgtg aacgtcgtgg ggcggctgcc gctggagctc aacctcgcct tcacctcgct
     8881 caacatcgcc ttcagcgccg ccgtcgccat gctcgtgcgc acgttctgcg gggcggcgct
     8941 ggacctcggc cggcttcgcc gcttcctgcc cttcctggcc atcgtcgcct gcgcggccct
     9001 ggtcgagggc atgatcggct cgtcgatgat ctcgacgtcg cccgagcagt tcctgaccat
     9061 ctggcggcgc tggatggcct gcgacggcgt gggcatggtg ctgggcctgc ccgccaccct
     9121 gatgatgatg cggggtgtca acccgctgca cgccggcccg gccgacgcac gcgagcgggt
     9181 cgcgctgctg gcggtcctgg gcctgggaac ctacgccgcc ttcgccgtgg cggcggcccc
     9241 ggtgttcctg ctgatctttc ccgccctgct gttcgccgcc ttccgcctgg gctcgggctg
     9301 ggccttcctg gcggtgtgga tctgcggcca ggcggcgacg atccagaccc tggccgggcg
     9361 cggaccgatc gccgtgttcg acggcccccc gccgttcatc gagctgggcc tgctgcagct
     9421 gttcctgctc agcatcctgt tgaccgcctc gctcgcgacc agcgccctgg ccgagcgcat
     9481 gcgggccgag cagagactgc gacgcaaggc cgccgccacc gccgcggccc gggcgcgcat
     9541 cgatcaggtc acggcgacgc gcgagcggtt cctggccgtt atcagtcacg aaatccgcac
     9601 cccgctgaac ggtatcgtcg ggttcggcga ggcgctggcc aagcgcgcgg acctcgatcc
     9661 cgaggcccgc caccaggtcg agatgatcgg tcgatccacg gaagcattga tgggcatcgt
     9721 cgacgacatc ctcgactacg cccggatcga atcgggccga ctcgacatca agcccgaggc
     9781 cgcctcggtc gagcagacgc tggcccgggc cgtggaggcc gccgcgccgc tggcgatcgc
     9841 caagggcctg accctgaccc gaacgggggt cgcgctggag gacgccctgc atcgggtcga
     9901 cgcccgtcgc ctgcagcagg tgttcggaca tctgatcgac aacgccgtca agttcaccgc
     9961 ggcgggtgga gtcgaggtgc gcctggagtg cgcgccggcc ggcgacgatg agaagttgac
    10021 tgtcacggtt tccgacagcg gccagggcgt gcccgagcac cagcgacgcc acctgttccg
    10081 gccgttcgcc cagctcgacc cgtcgctgtc gcgccggcac ggcggggccg gcctcggact
    10141 gtcgatctgc cgggcgatcg ttgacctgat ggacggcgag atcagctacc ggcccgggcc
    10201 ggccggcggt tcggtgttca gcatgtccgt caccctgccg cgcgccggtg tcgccacctc
    10261 gcaggacgag gggctcggcg agcgggctcc gcgggtgctg gtggtcgacg accatccgat
    10321 gaaccgggaa gtggcgcggc tgttcctcga cgccttcggc tgcgaggtca gtgagtgcga
    10381 agacggcggg gcggccgtcg aggcggtcgc gggcggcgcc ttcgacgtga tcctgatgga
    10441 cgtacgcatg cccggtgtcg acgggctgtc cgcgacccga atgatccgcg agctgggcgg
    10501 gccggcgtcg ctgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnngggcggc cggcatgaac ggtcacgtct ccaagccgat cgaccaggcg
    10621 cggctgctca acgccctcag ctgcgccctg cagggtcggg acgccttcgc cgccgacctg
    10681 gccgcctgac cggtcaggcc aggtctcgcg gctccggcca ggggaacgcg cgggcctggc
    10741 gcacgaacgc gccgacggcc ggggcatggg ggcggccacg cacggtgacc agcatgatgg
    10801 tccgttcgaa ctccgggtcg atcagcttgc ggatcaccag ggccggatca gtgacgctga
    10861 actcgggaat gaagccgaga ccgaggccgg ccttgatcat gccctggacc cagtcgtcgc
    10921 gctcgctgga gaataccttg cgcatgacca cgccgcggcc gcggaactgt cgcccggcgt
    10981 ggtcgaaata ctcgcagctg gcccggctga cgtagttctc gtcatgcagg tccgccgccc
    11041 ggacgacgtt ctgggcggcg aaccggtggt cgcgcgggac ggccaccacg aaacgctcgc
    11101 gaaacagcgg cagggcatgg aaccggtcgt cgaccccgtc gggcagtccg aacagggcca
    11161 ggtcgagatc accggcctcg agccggcccg ccagctcggc ggcggccatg tcgctgaccc
    11221 gcagatcgac cccgggattg gccccgcgga aggcgaccac cagatcggcg atgatcgccg
    11281 gccccacggt gcacatgacg ccgatatgca gcgtggcccg gtcgagcgcc ttaagcgatc
    11341 gggcctccga ccgggccgcc tcggcctggg ccaggatgcc ctcgaaatag ggcagcatgg
    11401 tccggcccag ctcggacagg tgggtccgcc ggccctcgcg atggaacagc ggcccgccga
    11461 gctcggcctc gagttgctgg atcgcccggg tcagggccgg ctgcgtgacc cccgaccgtt
    11521 cggcggcgcg ggtgaagttg agcgtgtcgg ccagcgccag gaagtaccgg acctgctgca
    11581 tttccatcgc ggtagccccc cgatcaacgc agaatcgtag cgtcggccgc gccggcgaca
    11641 aaacacccga ttgaggcgcc gtagcggcaa ggcgctatag cccggagacg aagcagatgg
    11701 agcccgcatg gccgacgacg attcgactgg tgttgaggat ctcaaccgca ccctggggct
    11761 ggtccgccgg atcgagatga acccggaggg ccgcgcggtc atcgaatacg aggccggccc
    11821 gcacatgtgc cactcggggg gcgtggtcca gggcggcttc gtgaccggct ggatcgacgc
    11881 ggccatggcc cacgccgcga tcgccatgac gggaccgggc gtcgtgccca tgtcgctgga
    11941 gctgaaggtc agcttcttcg cgccggcccg gccggggcgg gtgatcgccg aggcctgggt
    12001 cgagcgtcgc ggacgcacga cctgcttctt cgagggccgg ctgctcgatc cggccggcaa
    12061 ggtgctggcc aaggccagct cgacgctgca gctggtcgac cgcgccaagg tcgaggcggc
    12121 cagtcgcgcc gcggcgacct gacaggattt ctggctggac ttcgctgtcc ggcgccatga
    12181 cacagcctcg cagcctgccg tcagagcggg cgcgatcaca tcccgggatg gagacaagat
    12241 gcactacgcc gaactgaacg aggcctgggc cgaactgacc gcgccgggcg cgcccttcga
    12301 gatcgccgag atcgaggtcc gcggcgcgcg catccgctca ttccggaacg cgccgccgag
    12361 cgttcgcgag gtgtggctgt ccaccctgcc gttcggcgag cgtgactacc tcgtctacga
    12421 ggacgaacgc atcacctacg gcgaggcgca ccggcaggtc gcttccatcg ccaactggat
    12481 gatggcccat ggcgtgaagc cgggcgaccg cgtggccatc gccatgcgca actatcccga
    12541 atggatgctg atctact
//
LOCUS       DAORNX010000026        12469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3827_length_12469_cov_8.148461, whole genome shotgun sequence.
ACCESSION   DAORNX010000026 DAORNX010000000
VERSION     DAORNX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_12469_cov_8.148461"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..360)
                     /locus_tag="PLO65_02150"
     CDS             complement(4..360)
                     /locus_tag="PLO65_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27081.1"
                     /translation="MNSIVRAASAIALAALLAGCAGMITGGPLIRPGEPVGAIAVINR
                     TSHTIGVVSISACSASTYGLNRLPDGVSIASGRSYQFQVSAGCWDVDAGAFGVGEARQ
                     RLTVSPGGVTRYTVTD"
     gene            complement(434..1483)
                     /gene="selD"
                     /locus_tag="PLO65_02155"
     CDS             complement(434..1483)
                     /gene="selD"
                     /locus_tag="PLO65_02155"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRD27082.1"
                     /translation="MSDPVRLTSLAHGGGCGCKLAPAVLREILAKTPAAAAFPNLMVG
                     TETSDDAAVWRLNDQQALVATTDFFMPVVDDPFDFGRIAATNALSDVYAMGGTPIFAL
                     ALVGMPVDRLPVGTIQQILAGGASVCAAAGVPIAGGHSIDSVEPIYGLVALGLVHPDK
                     VLTNRGARAGDVLILTKALGVGILSAAFKQEKMPAGGYEAMIASTTQLNTIGASLGDT
                     PGVHAVTDVTGFGLLGHLLEMCRGGGLAAEIDPDAPALLTGVEALASGGVRTGASTRN
                     WASYGEAVRLPDSFDDWRRDILCDPQTSGGLLIAVAEDQAEAVLDRALAAGFGASRRI
                     GRLVEGAPEVRVGTG"
     gene            1576..2640
                     /gene="mnmH"
                     /locus_tag="PLO65_02160"
     CDS             1576..2640
                     /gene="mnmH"
                     /locus_tag="PLO65_02160"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRD27083.1"
                     /translation="MSRIQPIQSADAATLARFATIIDVRSPGEFALDHVPGAVNLPVL
                     TDAERAEVGTIYVQESRLKANRIGAAYVARNIAGYLVNELAGMPQDFAPLVYCWRGGS
                     RSESMATILSRVGWRTSLLVGGYKTYRRHVQARLYDEAWPLNLMLLDGDTGTGKTGIL
                     GRLAARGLQTLDLEGMAGHRGSLFGGLADRPQPNQKGFESTLLRAMEALDPARPVVVE
                     AESSRIGDLMVPPALWARMQAAPRLEVTAPREERARYLVRAYSDIVANHDLIEAVLTR
                     LPVHPSQTRLEGWRALLAAGDYAALAEALMELHYDPAYRRGRRKDGREPIGVIALETL
                     DGANLDLAADEIARLVEPKT"
     gene            complement(2711..3946)
                     /locus_tag="PLO65_02165"
     CDS             complement(2711..3946)
                     /locus_tag="PLO65_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27084.1"
                     /translation="MSIDFEIPPEARAIREKVRQWVNDECKPAEKALLAGGDYKTILG
                     ELRTKARAQGLWCPFIPKEHGGMGLGPLANALVQMELGESYLGALSMNTQGPDDATMM
                     TLLAHGTEYQKEKFLKPLLNGEKRICYSMTEKAAGADATGMQTRAVKDGNENYILNGE
                     KWFSSAASVADIALVMAKTDPDAPRHQQFSTFIVELPNPGYRIVRDIETMAAEGPLSH
                     ILGGGHAEVEIKDLVVPAENLLGGEGNGFNMGQHRLAYGRLRHGMHNVSMAQRALDLA
                     VAHVTSRSTFGKPLHERQGVQFMLADCAQDIYIARLMLLHIAYKAEKGMDLRQENGIA
                     KVYLANMVHRVVDTAIQLHGALGYSMDTPLARWYTHIRSQRLVDGPDEVHRWTTGRNV
                     IKAFQRDGTTAAACGGELL"
     gene            complement(3979..4785)
                     /locus_tag="PLO65_02170"
     CDS             complement(3979..4785)
                     /locus_tag="PLO65_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27085.1"
                     /translation="MGNSQAGKTLFVAGGSSGINLGIARKFAAEGANVALISRSPEKI
                     AAAAAEIAGINGGRAIGIAADVRDYAAVEAALKQTHDTYGDIDTVISGAAGNFVAPAL
                     GMSANGFKTVVDIDLIGTFNVLRASFQFLRRPGASLISITAGQATRASMFQAHVCAAK
                     AGINMLTQCLALEWGPAGVRVNAISPGPIADTEGMARLATTPEAEARVKARVPLRRYG
                     TKDEIGDAALFLSSDAARYINGAILPVDGGSGCGDGSGDALGNFAAAAFS"
     gene            complement(4846..5985)
                     /locus_tag="PLO65_02175"
     CDS             complement(4846..5985)
                     /locus_tag="PLO65_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27086.1"
                     /translation="MTYRLYGTPGSLYTAKARSWLIKNGVPFENRAAGEPRFREEIVP
                     KIGRWIIPVLEAPDGELIQDGSAIIARLEADGLARWPTVPATPRHRVIGQVFELFGGE
                     GLLRPAMHYRWNFDETNLPFIGLDFAAGLATPGADPAEGAAVFQFAAQRMRKAMASFG
                     VSEASIPLVERSYVRFLDLFEAHLAGSPYLLGGRPTIGDYGLIAPLYAHLGRDPHPAD
                     LMKRKAHRVWRWVERMNAADLDSGEYGYPPPELFPDDGVPDTLKALLRFVAEDYLPEV
                     RAYVGFANAWLAERPDLEPMTSGLARSSERVIGRTAFDWRGESIEVAVMPYRLWLLQK
                     VQDAAADSPVSGRLLAETGLADLLTLTTSRRVERVNQLEVWTAPL"
     gene            6122..7546
                     /locus_tag="PLO65_02180"
     CDS             6122..7546
                     /locus_tag="PLO65_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD27087.1"
                     /translation="MSSETTYTMTIGGKAVAGAGRFDVLNPANEQVIAQAPDCSREQL
                     DQAVAAARAAFPAWRATPIAKRREALLAIAGVLAGNVEELKRLLTAEQGKPHADAMGD
                     ILGGAYWCQATASLDLPVTVVEDTAERRGETRHVPIGVVGAIAPWNFPIILAMFKVAP
                     ALLAGNTVILKPSPFTPLTTLRVGELLRGVLPDGVLNIVTGGDDLGPWITSHPGIDKV
                     SFTGSTQTGRKVMQSAAATLKRITLELGGNDAAIVMPDVDVEKVAEQLFWAAFKNTGQ
                     ICIATKRMYVHKDVYEPLKDALVAYAKTVKMGDGAEQGVQMGPIQNRLQYQRVLDLIA
                     DAKANGYKFLIGGETSEAPGYFVPVTIIDNPPENARIVQEEQFGPVLPLLKWDDLDDV
                     VGKANATEYGLGGSIWSSDEQKALDIAERLATGTVWINESQHLSPLAAFGGQKQSGLG
                     VEGALEGLLEYTNTQTVFLKKAAA"
     gene            7546..8295
                     /locus_tag="PLO65_02185"
     CDS             7546..8295
                     /locus_tag="PLO65_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD27088.1"
                     /translation="MSDLVLREDRGAAAILTLNRPDKLNSLNVPVFEALEGHVAALED
                     DPGGASVVVLRGAGRCFSAGHDLGDIAVGEKLPRPNYQSHVIERLANLPLPVISAVHG
                     HCYTGALELALAGDIILAAESAKFADTHARWALTPVWGLSQRLPRRVGTYKAREMMFT
                     CRTYSGREAEAMGLANACAPDDGFEAAIDAWVAEIAANSPFSHAANKRLVTHTDGLPI
                     AAGLAHEVYHGEGVGPDMRDRIAAFAARARR"
     gene            complement(8332..10677)
                     /locus_tag="PLO65_02190"
     CDS             complement(8332..10677)
                     /locus_tag="PLO65_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27089.1"
                     /translation="MKATAFGRRATVWSSVLLASTMLTGVSAAFAQDTTVGEIIVTAQ
                     KRAEDLQDVPVSIQAIGGEKLEALVISDFNDYVKFLPSVSFQSTAPGFSSVYMRGVAS
                     GGDGNHSGSLPSVGIYLDEQPITTIQGALDVHVYDMERVEALAGPQGTLYGASSQAGT
                     IRLITHKPEIGDFSGAIDTEVNSVSEGDIGYGVEGFVNVPVSDSAALRVVGWYQHDAG
                     YVDNVLSSRTYPTWGGTITNTRFAEDDYNDVTTTGGRMALKIDLNEDWTVTPSLMGQT
                     ETSHGNFSFDPTLGDLKIARYRPESAKDKWYQAAMTVEGKIGSLDFVYAGSYMKRDVD
                     SQSDYSDYSFFYDTLFGYGAYWVDDLNNPVEPTQYIKARDRYTKESHEIRLSTPSDLP
                     LRVVGGYFFQNQTHGIEQRYMIDGIGSAIEVSGWPDTIWLTQQFRTDKDYALFGEVAW
                     DFHPGWTLTGGVRYFLSENGLKGFFGYGAGYSSSTGEAACDPSLPVYSTAPCTNLHKT
                     TEESGFTHRVNLTWSVDDDRMLYGTWSTGYRPGGINRRGTLPPYSSDFLTNYELGWKT
                     MWFDRSVRWNGAIYLERWDDFQFSILGANGLTEIKNAAQARIRGLETDVTWRVAEGLT
                     INGGAAWTQAELTANYCGFTDPQGNPVTQCAVPQAPKGTQLPVTPRFKGNVTVRYEFA
                     LGEYDAHLQGSMVTQTSSWTDLRLVERAIIGKQDGWSAFDFTAGVERDAWSAEFFVQN
                     LTDERASLYRYAQCAESVCGGAVYTVPNQPRTLGLRLGRKF"
     gene            complement(10816..>12469)
                     /locus_tag="PLO65_02195"
     CDS             complement(10816..>12469)
                     /locus_tag="PLO65_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD27090.1"
                     /translation="ADQLALAGDEAGAEAARAQQIRASASDPDLLEAGVALCDNRLAV
                     AERLLKPYLARHPTNAAAIRMLAEVAARLGRNEDAETLLARCVELAPAFAEARHNHAT
                     ILYRLNRSEEALAQLDWLLARNPQHPGYRNLRAAALGRVGDYQGAIALYDELLKAYPD
                     QPKAWMSYGHSLKTVGRQSDAVAAYRRSIALAPQMGEAWWSLANLKTVRFSDDDVAAM
                     EGQLARQDLADEDRFHLQFALGKALEDAGQAARAFAFYRDGAALRRKAIDYDAEANTA
                     HAARSKALMTPAFFAERRDWGCPAPDPIFVVGLPRSGSTLIEQILSSHSAVEGTMELP
                     DIISMARRLGGRQRRGQPSAYPEVLGTLDADACRALGDEYLDRTRIQRKLGRPLFIDK
                     MPNNFAHIGLIRLILPRAKIIDARRHPLGCCFSAFKQHFARGQTFSYDLVELGRYYAD
                     YVDLMAHWDAAIPGAVHRVIYERMVADPEAQVRRLLDHCGLPYEPACLAFHENDRAVR
                     TASSEQVRRPIFTDAVDHWRAYEAWLDPLKTALGPALDTWDA"
BASE COUNT         1901 a         4210 c         4424 g         1934 t
ORIGIN      
        1 gacctagtcg gtgaccgtgt agcgggtgac gcccccgggg ctgacggtca ggcgctggcg
       61 cgcctcgccc acgccgaagg ccccggcgtc gacatcccag catccggccg agacctggaa
      121 ctggtagctg cggcccgacg cgatcgacac gccgtccggc aagcggttga gaccgtaggt
      181 cgacgcgctg caggcggaga tcgagaccac gccgatggtg tggctggtgc ggttgatgac
      241 ggcgatcgct ccgacgggct cgccggggcg gatcagcggc ccgccggtga tcatcccggc
      301 gcaaccggca agaagcgcgg caagggcgat agcggacgcg gcgcggacga tgctgttcac
      361 ggtgggctcc cttcggtgac cggcccagtc tgggtcggcc cgggagaacc ggcatgctcc
      421 gaaagtgggg gattcagcct gttccgacgc ggacctccgg cgcgccttcg accagtcgtc
      481 cgatccggcg gctggcgccg aagccggcgg ccagcgccct gtccagcacc gcctcggcct
      541 ggtcctccgc cacggcgatc agcagaccgc cgctggtctg ggggtcgcag aggatgtcgc
      601 gccgccagtc gtcgaagctg tccggcagcc gcaccgcctc gccgtagctg gcccagttgc
      661 gcgtcgaggc gccggtgcgc acgccgccgc tggcgagggc ttccacgccg gtcagcagcg
      721 ccggggcgtc ggggtcgatc tcggcggcaa ggccgccccc ccggcacatc tccagcagat
      781 ggcccagcag gccgaagccg gtgacgtcgg tgaccgcgtg cacgcccgga gtgtcgccca
      841 ggctcgcacc gatggtgttc agctgagtgg tcgaggcgat catcgcctcg tagccgccgg
      901 ccggcatctt ctcttgcttg aaggcggcgc tgaggatgcc gacgccgagc gccttggtca
      961 ggatcagcac gtcgccggcg cgggcgccgc ggttggtcag caccttgtcg gggtggacca
     1021 ggcccagggc gaccaggccg tagatcggct cgacgctgtc gatcgaatgg ccgccggcga
     1081 tcggcacgcc ggccgccgcg cagaccgagg ccccgccggc gagaatctgc tggatcgtcc
     1141 cgaccggcag gcgatccacc ggcatcccga ccagggccag ggcgaagatc ggcgtgcccc
     1201 ccatggcgta gacatccgac agggcgttgg tggccgcgat gcggccaaag tcgaacgggt
     1261 cgtcgaccac cggcatgaag aagtcggtgg tcgccaccag ggcctgctgg tcgttgaggc
     1321 gccagaccgc cgcgtcgtcc gaggtttctg ttccgaccat caggttcggg aaggccgccg
     1381 ccgccggcgt cttcgccagg atctcgcgca gcaccgcggg cgccagcttg cagccgcaac
     1441 cgccgccatg ggccagcgag gtcagtcgca cggggtcgga cacggggctt actccttcgg
     1501 gctcgccgct tcatattgaa tgacgggggc gaagtcccct acagcggagg ggatacgggc
     1561 ggaaacaggt catccatgtc gcgcatccag ccaatccaga gcgccgacgc cgccaccctg
     1621 gcgcggttcg cgacgatcat cgacgtgcgc agccccggcg agttcgcgct cgaccatgtg
     1681 ccgggggctg tcaacctgcc ggtgctgacc gacgccgagc gggcagaggt ggggaccatc
     1741 tacgtccagg aatcccgact caaggccaac cgcatcggcg ccgcctacgt ggcgcgcaac
     1801 atcgccggct acctggtcaa tgagctggcc gggatgccgc aggacttcgc gccgctggtc
     1861 tactgctggc gcggcggatc gcgctcggag tcgatggcca cgatcctttc gcgggtgggc
     1921 tggcgcacca gcctgctggt gggcggctac aagacctacc ggcgacacgt ccaggcgagg
     1981 ctgtacgatg aggcctggcc gctgaacctg atgctgctcg acggcgacac ggggaccggc
     2041 aagaccggga ttcttggccg gctggccgcc cgcggcctgc agacactgga cctcgagggc
     2101 atggccggcc atcgcggctc gctgttcggc ggtctggcgg accggccgca gccgaaccag
     2161 aagggcttcg agtcgacact gctgcgggcc atggaagcgc tggatccggc gcgcccggtg
     2221 gtcgtcgagg ccgagtcgag ccgcatcggc gacctgatgg tcccgccggc cctgtgggcg
     2281 cggatgcagg cggcgccgcg ccttgaggtg accgcccccc gcgaggagcg ggcccgctac
     2341 ctggtgcgcg cctacagcga catcgtcgcc aaccatgacc tgatcgaggc ggtgctgacc
     2401 cggctgccgg tgcatcccag ccagacgcgg ctggagggct ggcgcgcgct gctggcggcc
     2461 ggcgactatg cggccctggc cgaggcgctg atggaactgc attacgatcc cgcctatcgc
     2521 cgcggccgcc gcaaggacgg ccgcgagccg atcggggtga tcgcgctgga aacgctggac
     2581 ggcgcgaacc tggacctggc cgcggacgag atcgcgcggc tggtcgaacc gaagacataa
     2641 acagtcatcc cggccgtagc gtggcggaga gccggggccc agattcggcc gggatgacag
     2701 tttctgggcc ctaaagcagt tccccgccac aggcggcggc cgtggtgccg tcgcgctgga
     2761 aggccttgat cacgttgcgg ccggtggtcc agcggtggac ctcgtccggc ccgtcgacca
     2821 ggcgctggct gcggatgtgg gtgtaccagc gggccagggg ggtatccata gagtagccca
     2881 gggcgccgtg gagctggatc gccgtgtcga ccacccggtg gaccatgttg gccaggtaga
     2941 ccttggcgat gccgttctcc tggcgcaggt ccatgccctt ctccgccttg taggcgatat
     3001 gcagcagcat cagccgggcg atgtagatgt cctgggcgca gtcggcgagc atgaactgga
     3061 cgccctggcg ctcgtgcagc ggcttgccga aggtcgagcg gctggtgaca tgggccacgg
     3121 ccaggtcgag ggcccgctgg gccatcgaga cgttgtgcat gccgtgacgc aggcggccgt
     3181 aggccaggcg gtgctggccc atgttgaagc cgttgccctc gccgccgagc aggttctcgg
     3241 ccggcacgac caggtccttg atctcgacct cggcgtggcc gccgccgaga atgtgcgaca
     3301 gcgggccttc agcggccatg gtctcgatgt cgcgcacgat gcggtagccg gggttgggca
     3361 gctcgacgat gaaggtcgag aactgctggt gccgcggcgc gtccggatcg gtcttggcca
     3421 tgaccagggc gatgtcggcg acgctggcgg ccgaggagaa ccacttctcg ccattgagga
     3481 tgtagttctc gttgccgtcc ttgaccgcgc gggtctgcat gcccgtggcg tcggcgccgg
     3541 cggccttctc ggtcatcgag tagcagatgc gcttctcgcc gttcagcaac ggcttgagga
     3601 acttctcctt ctggtactcg gtgccgtgcg ccagcagggt catcatggtg gcgtcgtcgg
     3661 gaccctgggt gttcatcgac agggcgccga gatagctttc gcccagctcc atctgcacca
     3721 gggcgttggc cagcgggccg agacccatgc cgccatgctc cttggggatg aacgggcacc
     3781 acaggccctg ggcgcgggcc ttggtccgca gttcgcccag gatggtcttg tagtcgccgc
     3841 cggcgagcag ggccttctcg gccggcttgc actcgtcgtt gacccactgg cggacctttt
     3901 cgcggatggc cctggcctcc ggcggaatct cgaagtcgat ggacatcggt tctctccctg
     3961 ttgttctttt cctgtggttt agctgaaggc cgcggcggcg aaattgccca gggcgtcgcc
     4021 ggacccgtcg ccgcaacccg acccgccgtc gaccggcagg atggcgccgt tgatgtagcg
     4081 ggccgcgtcg gagctgagga acagggctgc gtcgccgatc tcgtccttgg tcccgtagcg
     4141 gcgcagcggc acccgcgcct tgacccgcgc ctcggcctcc ggagtggtgg ccagacgcgc
     4201 catgccctcg gtgtcggcga tcgggccggg cgagatggcg ttgacccgca ccccggccgg
     4261 cccccactcc agcgccaggc actgggtcag catgttgatg ccggccttgg cggcgcagac
     4321 gtgggcctgg aacatcgagg cgcgcgtggc ctgtccggcg gtgatcgaga tcagcgaggc
     4381 gcccggacgg cgcaggaact ggaacgaggc gcgcagcaca ttgaaggtgc cgatcaggtc
     4441 gatgtcgacc accgtcttga acccgttggc cgacatgccc agcgccgggg cgacgaagtt
     4501 gccggccgcg ccggagatca ccgtgtcgat atcgccgtag gtgtcatggg tctgcttcag
     4561 ggcggcctcg acggcggcgt agtcgcgcac gtcggcggcg atgcctatgg cccggccgcc
     4621 gttgatgccg gcgatctcgg cagccgcggc cgcgatcttc tcggggctgc ggctgatcag
     4681 ggcgacattg gccccctccg cggcgaactt gcgggcgatg cccagattga tgccgctcga
     4741 tccgcccgcg acgaacagcg tcttgccggc ctgactgttt cccatggtgt ctctccctgt
     4801 tgcggcgatg atgggcgggc gccgcatggg aagacaaggg cgagctcaga gcggcgcggt
     4861 ccagacctcc agctggttga cccgttcgac ccgacggctg gtcgtcagcg tcagcaggtc
     4921 cgccagaccc gtttccgcca gcaggcggcc tgagaccggg ctgtccgcgg ccgcgtcctg
     4981 caccttctgc agcagccaaa ggcggtaggg catgactgcg acctcgatcg actcgccacg
     5041 ccagtcgaag gcggtccggc cgatgacccg ttcggaactg cgggccagac cgctggtcat
     5101 gggctccaga tcgggccgtt cggccagcca ggcgttggcg aagccgacat aggcgcgcac
     5161 ctccggcaga tagtcctcgg cgacgaagcg cagcagcgcc ttgagggtgt ccggcacgcc
     5221 atcgtcggga aacagctccg gggggggata gccgtactcg cccgagtcga ggtcggcggc
     5281 gttcatccgc tcgacccagc gccagacgcg gtgcgccttc cgcttcatca ggtcggccgg
     5341 atgcgggtcg cgacccaggt gggcgtagag cggcgcgatc agtccgtagt cgccgatcgt
     5401 cggccgcccg ccgagcaggt agggcgagcc ggccagatgc gcctcgaaca ggtccaggaa
     5461 ccgcacgtag gatcgttcga ccagcggaat ggacgcctcc gacaccccga aggaggccat
     5521 cgccttgcgc atcctctggg cggcgaactg gaacaccgcc gccccctcgg ccggatcggc
     5581 gcccggcgtc gccagcccgg cggcgaaatc cagcccgata aatggcaggt tggtctcgtc
     5641 aaagttccag cggtagtgca tggccggccg cagcagtccc tctccgccga acagctcgaa
     5701 gacctggccg atcacccgat ggcgcggcgt ggcgggaacg gtcggccagc gggcaaggcc
     5761 atcggcctcc agccgggcga tgatcgccga gccgtcctgg atcagttcgc cgtccggcgc
     5821 ctccagcacg gggatgatcc agcgaccgat cttcggaacg atctcctcgc ggaagcgcgg
     5881 ctccccggcc gcgcggttct cgaacggcac accgttcttg atcagccagg accgcgcctt
     5941 ggccgtgtag agcgacccgg gcgtgccgta gagtctgtag gtcatcgccg aagccccctg
     6001 ccaaccgtga caactatctg cattggcaat gccaattgat caagcggcgc ggacagcgac
     6061 cccgcttggc tgaccaagcg tcagcgagtc attgtgccga caacgacaag ggagcccgga
     6121 aatgtccagc gagaccacct acaccatgac catcggcggc aaggccgtcg cgggcgccgg
     6181 ccggttcgac gtgctcaacc cggccaacga acaggtcatc gcccaggcgc cggactgctc
     6241 gcgcgagcag ctcgaccagg cggtggccgc cgcccgggcc gcctttccgg cctggcgcgc
     6301 cacgcccatc gccaagcgtc gcgaggccct gctggccatc gccggggtgc tggccggcaa
     6361 tgtcgaggag ctcaagcgcc tgctgaccgc cgaacagggc aagccgcacg ccgacgccat
     6421 gggcgacatc ctcggcggcg cctactggtg ccaggcgacc gccagcctcg acctgccggt
     6481 gacggtcgtc gaggacacgg ccgaacgccg cggcgagacc cgccatgtgc cgatcggcgt
     6541 ggtcggcgcc atcgcgccgt ggaacttccc gatcatcctg gcgatgttca aggtcgctcc
     6601 ggccctgctg gccggcaaca cggtgatcct caagccctcg ccgttcacgc ccctgaccac
     6661 cctgcgcgtt ggcgagctgc tgcgcggggt gctgccggac ggcgtgctga acatcgtcac
     6721 gggcggtgat gacctcggtc cctggatcac cagccatccg ggcatcgaca aggtcagttt
     6781 caccggctcg acccagaccg gccgcaaggt catgcagtcg gccgccgcga cgctgaagcg
     6841 catcacgctc gagctcggcg gcaacgacgc ggccatcgtc atgcccgacg tcgacgtcga
     6901 gaaggtcgcc gagcagctgt tctgggccgc gttcaagaac accggccaga tctgcatcgc
     6961 gaccaagcgg atgtacgtgc acaaggacgt ctacgagccg ctcaaggacg cgctggtcgc
     7021 ctatgcgaag acggtgaaga tgggcgacgg cgccgagcag ggcgtgcaga tggggccgat
     7081 ccagaacagg ctgcagtacc agcgggttct ggacctgatc gccgacgcca aggccaacgg
     7141 ctacaagttc ctgatcggcg gcgagacgtc cgaggcgccg ggctacttcg tgcccgtgac
     7201 gatcatcgac aacccgccgg aaaacgcccg catcgtgcag gaagaacagt tcggcccggt
     7261 cctgccgctg ctgaagtggg acgacctgga cgacgtggtc ggcaaggcca acgccaccga
     7321 atacggcctg ggcggctcga tctggtcgtc ggacgaacag aaggcgctgg acatcgccga
     7381 gcggctggcc accggcacgg tctggatcaa cgagagccag cacctttcgc cgcttgcggc
     7441 cttcggcggc cagaagcagt cgggcctcgg cgtcgagggg gcgctcgagg gcctgctcga
     7501 atacaccaac acccagacgg tgttcctgaa gaaggcggcg gcctgatgag cgacctcgtc
     7561 ctgcgggagg atcgcggcgc ggcggcgatc ctgaccctca accggccgga caagctgaac
     7621 tcgctcaacg tgccggtgtt cgaggcgctg gagggtcacg tcgcggcgct ggaagacgat
     7681 ccgggcggag cgtccgtggt cgtcctccgc ggcgccggcc gctgtttctc ggccggccac
     7741 gacctgggcg acatcgccgt cggcgagaaa ctgccccggc ccaactacca gtcccatgtc
     7801 atcgagcggc tggcgaacct gccactgccg gtgatcagcg ccgtgcacgg ccactgctac
     7861 accggcgcgc tggagctggc cctggccggc gacatcatcc tggcggcgga gagcgcgaaa
     7921 ttcgccgaca cccacgcccg ctgggccctg acgccggtct ggggcctgag ccagcgcctg
     7981 ccgcggcggg tcgggaccta caaggcgcgg gagatgatgt tcacctgccg cacctattcg
     8041 ggccgcgagg ccgaggccat ggggctggcc aacgcctgcg cgcccgacga cggcttcgag
     8101 gcggccattg acgcctgggt cgccgagatc gccgccaact cgccgttcag ccatgcggcc
     8161 aacaaacggc tggtgaccca caccgacggc ctgccgatcg ccgcgggcct ggcccacgag
     8221 gtctatcacg gcgaaggcgt cggccccgac atgcgcgacc gcatcgcggc cttcgcggcc
     8281 cgggccaggc gctagccggc gcccccgcgg gggcgccgat ccgcatcggg cctagaactt
     8341 gcggcccagc ctcaggccga gggtccgcgg ctggttcggc acggtgtaga ccgcgccgcc
     8401 gcagaccgac tcggcgcatt gcgcatagcg gtagagactg gcgcgctcgt cggtcaggtt
     8461 ctggacgaag aactcggccg accaggcgtc gcgctcgacc ccggcggtga agtcgaaggc
     8521 gctccagccg tcctgcttgc cgatgatcgc ccgctcgacc agtcgcaggt cggtccagct
     8581 gctggtctgg gtgaccatcg agccctgcag gtgggcgtcg tattcgccca gggcgaactc
     8641 gtagcggacc gtcacattgc ccttgaagcg cggggtgacc ggcagctggg tgcccttggg
     8701 cgcctgcggg accgcgcact gggtcaccgg attgccctgc ggatcggtga agccgcagta
     8761 gttggccgtc agctcggcct gggtccaggc cgccccgccg ttgatcgtga gcccctcggc
     8821 gacccgccag gtcacgtcgg tctcaaggcc gcggatgcgg gcctgggccg cgttcttgat
     8881 ctcggtcagg ccattcgcgc cgaggatcga gaactggaag tcgtcccagc gctcgaggta
     8941 gatagcgccg ttccagcgca ccgaccggtc gaaccacatg gtcttccagc ccagctcgta
     9001 gttggtcagg aagtccgagc tgtagggcgg cagggtgccg cggcggttga tgccgcccgg
     9061 ccggtagccg gtcgaccagg tgccgtacag catccggtcg tcgtcgaccg accaggtcag
     9121 gttgacccgg tgggtgaagc ccgactcctc ggtggtcttg tgcaggttgg tgcagggcgc
     9181 ggtggaatag accggcaggc tcgggtcgca ggccgcctcg cccgtcgagc tgctgtagcc
     9241 ggcgccatag ccgaagaagc ccttcaggcc gttctcggac aggaagtagc ggacgccgcc
     9301 ggtcagggtc cagcccggat ggaagtccca ggccacctcg ccgaacagcg cgtagtcctt
     9361 gtcggtgcgg aactgctgcg tcagccagat ggtgtcgggc cagccagaca cctcgatggc
     9421 gctgccgatg ccgtcgatca tgtagcgctg ttcgatgccg tgggtctggt tctggaagaa
     9481 gtagccgccc accacccgca gcggcaggtc cgacggcgtg gacagccgga tctcgtggct
     9541 ttccttggtg tagcggtcgc gggccttgat gtactgcgtc ggttcgacag gattgttcag
     9601 gtcatcgacc cagtaggcgc cgtaaccgaa cagcgtgtcg tagaagaacg aatagtcgga
     9661 gtagtccgac tgcgaatcca cgtcgcgctt catgtatgaa ccggcataga cgaaatcgag
     9721 gctgccgatc ttgccctcga ccgtcatggc cgcctggtac catttgtcct tggcgctttc
     9781 cggccggtag cgggcgatct tcaggtcgcc cagcgtcgga tcgaacgaga agttgccgtg
     9841 gctggtctcg gtctgcccca tcagcgaggg ggtcacggtc cagtcctcgt tcaggtcgat
     9901 cttcagcgcc atccgaccgc cggtggtcgt gacgtcgttg tagtcatcct cggcgaagcg
     9961 ggtgttggtg atcgtaccgc cccaggtcgg gtaggtgcgg ctgctgagga cgttgtccac
    10021 gtagccggcg tcgtgctggt accagccgac cacgcgcagg gcggcggagt cgctgaccgg
    10081 cacgttgacg aagccctcga cgccgtaccc gatgtcgcct tcggagacgc tgttgacctc
    10141 ggtgtcgatg gccccgctga aatcgccgat ctccggcttg tgggtgatca ggcggatggt
    10201 cccggcctgc gagctggcgc cgtacagcgt gccctgcggt cccgcgagcg cctcgacccg
    10261 ctccatgtcg tagacgtgga cgtccagcgc gccctggatc gtcgtgatcg gttgctcgtc
    10321 gagatagatg ccgacgctgg gcagggagcc cgagtggttg ccgtcgccgc cgctggccac
    10381 gccgcgcatg tagacgctgg agaagcccgg cgccgtggac tggaacgaca cgctgggcag
    10441 gaacttcacg tagtcgttga agtccgagat gacgagcgcc tcaagcttct cgccgccgat
    10501 ggcctgaatg ctgaccggca cgtcctgcag gtcttcggcc cgcttctgcg cggtgacgat
    10561 gatctcgccg accgtggtgt cctgggcgaa ggcggcggac acgcccgtca acatcgtgct
    10621 ggccagcagc accgacgacc agactgtcgc cctgcgacca aacgccgtcg ccttcatcat
    10681 gactcgctcc cctgcgcgga caccccgttg agggatgtca gtgcgcggag ggccggactg
    10741 tcaagttaca gcgccgcaat tcagtaaatg caggaattca tgcctgcccc tgtggcgaca
    10801 atgtctcagg atcgctcagg cgtcccaggt gtccagcgcc ggcccgagcg cggtcttcag
    10861 cgggtcgagc caggcctcgt aggcacgcca gtgatcgacc gcgtcggtga agatcggccg
    10921 gcgcacctgt tcggaactgg cggtgcgaac ggctcggtcg ttttcatgga aggccaggca
    10981 ggccggctcg tacggcaggc cacagtggtc cagcaggcgg cgcacctgcg cctccggatc
    11041 ggcgaccatc cgttcataga tcacccggtg gacggcgccg gggatcgcgg cgtcccaatg
    11101 ggccatcagg tcgacgtagt ccgcgtagta ccggcccagc tcgaccaggt cgtaggaaaa
    11161 ggtctggccc cgcgcgaaat gttgcttgaa cgccgagaag cagcaaccga gcgggtgacg
    11221 ccgggcgtcg atgatcttcg cccgcggcag gatcaggcgg atcagcccga tgtgggcgaa
    11281 attgttgggc atcttgtcga tgaacaacgg ccggccaagc ttgcgctgga tgcgggtgcg
    11341 gtcgaggtat tcgtcgccca gggcccggca ggcgtcggca tcgagcgtcc ccagcacctc
    11401 ggggtaggct gacggctggc cgcgccgctg ccgccccccc agccggcggg ccatggagat
    11461 gatgtcgggc agctccatgg tcccctcgac ggccgaatgg ctggagagga tctgttcgat
    11521 cagggtcgat cccgagcgcg gcaggccgac gacgaagatc gggtcggggg ccgggcagcc
    11581 ccagtcccgc cgctcggcga agaaggccgg ggtcatcagg gccttcgacc gcgccgcatg
    11641 ggcggtgttg gcctcggcgt catagtcgat cgccttgcgc cgcagggccg cgccgtcgcg
    11701 atagaaggcg aaggcgcgcg ccgcctggcc cgcgtcttcc agcgccttgc cgagggcgaa
    11761 ctgcaggtgg aagcggtcct cgtccgccag gtcctggcgc gccagctggc cctccatcgc
    11821 cgccacgtcg tcgtcggaga agcgcacggt cttgagattg gccaggctcc accaggcctc
    11881 gcccatctgc ggcgcgaggg cgatgctccg gcggtaggcc gcgacggcgt ccgactggcg
    11941 gcccacggtc ttcagcgaat ggccgtagct catccaggcc ttgggctggt cggggtaggc
    12001 cttcagcagc tcgtcataca gggcgatggc gccctggtag tccccgaccc ggcccagggc
    12061 ggcggcccgc aggttgcgat agccggggtg ttgcggattg cgggccagca gccagtccag
    12121 ctgggccagc gcctcctccg accggttcag gcgatagagg atggtggcgt gattgtgcct
    12181 cgcctcggcg aacgccgggg ccagttcgac gcaacgggcc agcagggttt cggcgtcctc
    12241 gttgcgcccc agccgcgcgg ccacctcggc cagcatgcgg atcgccgcgg cgttggtggg
    12301 atgccgggcc aggtagggct tgagcagccg ctcggccacg gccagccggt tgtcgcagag
    12361 ggcgacgccg gcctccagca gatctggatc ggaggccgag gcgcggatct gctgggcccg
    12421 ggcggcttcg gcgccggcct cgtcgccggc cagggccagc tggtcggcc
//
LOCUS       DAORNX010000027        12323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3881_length_12323_cov_7.664982, whole genome shotgun sequence.
ACCESSION   DAORNX010000027 DAORNX010000000
VERSION     DAORNX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_12323_cov_7.664982"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..560)
                     /locus_tag="PLO65_02200"
     CDS             complement(123..560)
                     /locus_tag="PLO65_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27091.1"
                     /translation="MVIFIKGRRALSVAAIATLLVALAQAIGTFAPQSAGDPALLSVV
                     AAMQGYRSPMGLGMEPSLLDIQTGLSLAMSLFLVAFAVLGLSFAGDRDASPRTLSRIA
                     VISAGTMGTLTVAFAVFRIPPLLVSMAVLAILFLVASLRTLRA"
     gene            complement(589..1539)
                     /locus_tag="PLO65_02205"
     CDS             complement(589..1539)
                     /locus_tag="PLO65_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HRD27092.1"
                     /translation="MKYRQLGSSDLMVSEISLGSWLTYGVGVERDNAIACVNRAFDLG
                     ITFLDTANVYGRGAAETVLGEALAGRPRDSYILATKLMGPMSDTDRGLSRAQVFKQID
                     ASLARLRTDYVDLYQCHRYDPDTPLEETMQALTEIVRAGKVRYIGFSEWSPDQIAAAL
                     AMPGVEKFVSSQPQYSLLWRRPEAKVIPLCAANGISQIVWSPLAQGVLTGKYRPGQPL
                     PEDSRAASDSMSSAIQRWMAPDILERVERLRPIVAEAGCTMAQFALAWVLREPNVASA
                     IVGASRPGQLDDNAAAVDLVIDPALFARAEAVLEPASAAT"
     gene            complement(1555..1992)
                     /locus_tag="PLO65_02210"
     CDS             complement(1555..1992)
                     /locus_tag="PLO65_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD27093.1"
                     /translation="MSSAVLPWQAWALLSAAFAALTAIFGKVGVEAINSDFATLIRTA
                     VILAVTAGIVAATGAWQPLSSVASRSWIFLTLSALATGASWLCYYRALKLGPAAQVAP
                     IDKLSIVLVAIFATLFLGEKLTGKNWLGVALIALGAILVATKR"
     gene            complement(2039..2638)
                     /locus_tag="PLO65_02215"
     CDS             complement(2039..2638)
                     /locus_tag="PLO65_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27094.1"
                     /translation="MTPFAFDTLSASKRLREAGMAEGVAEAVVAVFQHATASVDFGQL
                     ATKADLAGMATRADIADMVTRADIADMATKSDIAEMATKADIAEMATKADIAEMATKA
                     DIAEMATKADIADMATKSDIAEMATKADIADMATKSDLAALQAAIERDLVVQAAEAKL
                     GLGQLELRVQRRSDQQMWGIIGLLSGVIMLATATIKLFP"
     gene            complement(2674..4992)
                     /gene="clpA"
                     /locus_tag="PLO65_02220"
     CDS             complement(2674..4992)
                     /gene="clpA"
                     /locus_tag="PLO65_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HRD27095.1"
                     /translation="MPSFSRNLEETLHRAVAFANQRKHEYATLEHLLLSLTDDEDAAG
                     VMTACDVDLAALKKTLVNYLDTELRSLIVEDGEDAKPTAGFQRVIQRAVIHVQSSGRD
                     EVTGANVLVAVFSERESHAAYFLQEQDMTRYDAVNYIAHGIAKKAGASEPKQAKGANT
                     EGDEEKASVKTGGEALEAYCVDLNEKARQGKVDPLIGRHNEVERCIQILCRRTKNNPL
                     LVGDPGVGKTAIAEGLARKIVTHQVPEVLEGATIFALDMGALLAGTRYRGDFEERVKQ
                     VVKELENHPNAILFIDEIHTVIGAGATSGGAMDASNLLKPALASGSLRCMGSTTYKEF
                     RQHFEKDRALVRRFQKIDVNEPTIEDTLKILKGLKVYYEEFHKLKYTNDALKVAVDLS
                     AKYITDRKLPDKAIDVIDEAGAGQMLLPESKRKKTIGVKEIEAVVAKIARIPPKSVSK
                     SDTEALKELENDLQRTVFGQPEAIVQLSSAMKMARAGLRDPQKPIGCYLFSGPTGVGK
                     TEAARQLASTLGIELLRFDMSEYMERHTVSRLIGAPPGYVGYDQGGQLTDAVDQHPHA
                     VVLLDEIEKAHADVYNILLQVMDHGQLTDSNGKKVDFRNVVLIMTTNAGAADAQRQTI
                     GFGRGKAEGESEEAIKRIFTPEFRNRLDAVVAFKPLTQDIIRQVVQKFVLQLEAQLAD
                     RNVTIETSDEAADWLAKNGFDELYGARPLARVIQEHIKKPLADDILFGKLAKGGHVRV
                     VLKDGKIAFEFEVDDKPRQPGEPEIEEPALAE"
     gene            complement(5003..5332)
                     /gene="clpS"
                     /locus_tag="PLO65_02225"
     CDS             complement(5003..5332)
                     /gene="clpS"
                     /locus_tag="PLO65_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRD27096.1"
                     /translation="MAEKKSGAGSNGTGSAVVTQTKPKTAKPSLYRVLILNDDYTPME
                     FVVYVLERFFNKSREDATRIMLHVHQNGVGVCGVYTYEVAETKVAQVIDSARRHQHPL
                     QCTMEKD"
     gene            complement(5571..6836)
                     /locus_tag="PLO65_02230"
     CDS             complement(5571..6836)
                     /locus_tag="PLO65_02230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRD27097.1"
                     /translation="MKALCRDCFWTGDTAPRRCPSCSSPRVVSHDELATLSMAHLDCD
                     AFYASVEKRDRPELRDLPLIVGGGKRGVVTTCCYIARMSGVRSAMPMFKARQLCPQAV
                     VLPPEFARYRAESKRIFDKVRDLTPLVQPLSLDEAWIDLSGTERLHGAPPAVMLARLQ
                     AEIEAEIGLTVSIGLAANKFMAKIASEIDKPRGFSVVGAAGAQAFLAPRSVRILPGVG
                     PVMAAALEKAGFRTVGDIAAADLRELADRFGSHGLHLHKLSLGQDSRVVNPDQHRKTI
                     SAETTFFDDLHQVEDLENRLWPLCEKVARQLRAEGIAGRVATLKLRTPDFKIHTRRRT
                     LPVPTQTARTLFTVARELLAAEPAGRSYRLIGAGLSDFVEAAAAEGDFFADAEKRSLK
                     SETAIDALRARFGKGAVVTGRALGRQVRD"
     gene            6935..8104
                     /locus_tag="PLO65_02235"
     CDS             6935..8104
                     /locus_tag="PLO65_02235"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRD27098.1"
                     /translation="MSAVRSSEIRPEPSGEGLGLPGWIYHDPDFFEAEKRAVFRQSWQ
                     IVCHLSDIPQAGDFHTLEFLGESIVVVRGDDQKVRAFHNVCRHRASRVVDGPAGNCGL
                     RMTCPYHAWSYDLRGRLAGVPHVKAFEGFDQAAHNLAPVDIEVCLGFVFIRLEGGLPS
                     VREMLGPYYAEMEPYRMEDLQPLGRVTLREREVNWKNVSDNYSDSLHITVAHPGLTRL
                     FGAGYGLEAGEWCDKMWGALRDTPSDNWSERRYQAFLPVVEHLPPERRRLWTYFKLWP
                     NCAFDIYPDQIDFMQFLPVSPTRTVLREIAYVLPDDRREMCATRYLNWRVNRQVNIED
                     KALIERVQAGMASSSYAMGPLSVNEVALRSFTKRMRELIPQSREPVAPAPGWGRR"
     gene            complement(8258..9163)
                     /locus_tag="PLO65_02240"
     CDS             complement(8258..9163)
                     /locus_tag="PLO65_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRD27099.1"
                     /translation="MIRIGVDIGGTKVEAAALAADGSFVARVRQPNPGGYEAFLEVVR
                     DVVLEAGRQAGVRGASVGCGAPGSISPKTGLMRNANSVWMNGRPMREDLARVLGQPVR
                     LENDANCFALSEATDGAAAGARVVFGAILGTGCGGGVVVDGRTLEGRNGVGGEWGHSP
                     LPWSSKSEHQAHRCWCGRVDCLETWTSGSAFVADYREASGRTRDGAAIVAAARAGETA
                     AAEALDRYVDRLGRALASLCNLLDPDVIVLGGGMSNVDELYERVPPVVAHYIFSDTFE
                     TPIRQAKHGDSSGVRGAAWLWPLNS"
     gene            complement(9160..9798)
                     /locus_tag="PLO65_02245"
     CDS             complement(9160..9798)
                     /locus_tag="PLO65_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27100.1"
                     /translation="MQPVAHEDLVIRPTAPLLIVDVDEVLAHFMAGFERYVARHGVEM
                     RIDRFALFQNLYRPGETEPLDFETGKALFDDFFRHGADDLDPVEGAADALASLSQRAG
                     VVILTNAPAHALESRTGWLGKHGFDYPMILHSGLKGPAIAAMAGRTSGPAAFIDDLLP
                     NLESSAESAPAVATFQIIADHRLRPYAPTVPDRHDLHEDWSTLGPALGAAIR"
     gene            9917..10282
                     /locus_tag="PLO65_02250"
     CDS             9917..10282
                     /locus_tag="PLO65_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27101.1"
                     /translation="MAKKVLIVEDNELNMKLFHDLLDAQGYETLQTREGLQALALARE
                     HRPDLILMDIQLPEISGLEVTKWLKEDDELSHIPVVAVTAFAMKGDEERIREGGCEAY
                     ISKPISVSHFLDTIRRFLD"
     gene            10295..>10497
                     /locus_tag="PLO65_02255"
     CDS             10295..>10497
                     /locus_tag="PLO65_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27102.1"
                     /translation="MSARILVVDDIEANVKLLEAKLAAEYYEVLTASDGPTALAVAAA
                     ESPDIILLDVMMPGMDGFAVCRRL"
     assembly_gap    10498..10514
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10515..11649
                     /locus_tag="PLO65_02260"
     CDS             <10515..11649
                     /locus_tag="PLO65_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HRD27103.1"
                     /translation="PVVLVTALDGRSDRIEGLEAGADEFLTKPIDDVMLFARVRSLTR
                     LKQVMDELRAREASGRRIGVIAGAAARLGGSGGRVLVVDDNERQAQRLTAELAIEHRP
                     VIETDPEKAHLTARGPVDLIIVNATARSFDGLRFAANIRSDEATRHLPILAIVDPDER
                     HRLIKALEIGVNDLLTKPLDPQEMAARARTQIRRKRYTDFLRDNLDHSLELAVTDQLT
                     GLHNRRYMEGQLGALVKRASHGGDPVSALMIDIDHFKKINDAFGHDIGDEVLREFAVR
                     LASNVRAIDLPCRYGGEEFTVIMPGARLEDAQRVAERIRLHVAGSPFRVAGGKELLTV
                     TISVGVASTLGEADTPDALLKRADEAVYEAKAAGRNRVIARAA"
     gene            complement(11709..11876)
                     /gene="rpmG"
                     /locus_tag="PLO65_02265"
     CDS             complement(11709..11876)
                     /gene="rpmG"
                     /locus_tag="PLO65_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRD27104.1"
                     /translation="MAKPASIKIRLNSSADTGFFYVAKKNARTMTEKLVLKKYDPVIR
                     KHVEFKEGKIK"
     gene            complement(11952..>12323)
                     /locus_tag="PLO65_02270"
     CDS             complement(11952..>12323)
                     /locus_tag="PLO65_02270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD27105.1"
                     /translation="RRTGADPRLAAALASAALRETEEETGLVLPHEHGPLTLAARAIT
                     PTNSPVRFHARFFAVDAELARGEAADSGELADLAWRSFDESLALPLYDITEAVLRSLQ
                     TDGSPFMMTYRGGTTLVRALP"
BASE COUNT         1905 a         4124 c         4242 g         2035 t
ORIGIN      
        1 cctgtcgagg acgcgacgcc gcaggcggat ccggaaccgg cggccgacgc aacgccggcc
       61 gcctcgcccc aagagactcc cccggctgcg ccggacgcca cgccgccctc cgatcccctg
      121 atctaggccc gtagcgtcct cagcgacgcg accagaaaga gaatggccag caccgccatg
      181 ctgaccagca ggggcgggat gcgaaacacc gcgaaggcga cggtcagggt gcccatggtg
      241 ccagccgata tgacggcgat ccgcgacagc gtgcgcggcg aggcgtcccg gtcgccggcg
      301 aaggacagcc cgaggacggc gaaggcgacc agaaacaggc tcatggccag cgacagaccg
      361 gtctggatgt ccagcagcga tggctccatg cccagaccca tcggggaccg gtagccctgc
      421 atcgccgcga cgaccgacag aagggcggga tcgcccgccg actgcggtgc aaatgtcccg
      481 atggcctggg ccagggccac cagcagggtg gcgatggcgg cgacggacag cgcccggcgc
      541 cccttgatga aaatgaccat tatgtcctcc cggcgccgtt gatggtgatc aggtcgctgc
      601 ggacgcgggt tccagcacgg cctcggcccg cgcgaacagc gccgggtcga tgaccaggtc
      661 gaccgccgcg gcgttgtcgt cgagctggcc gggccggctg gcgccgacga tggccgaggc
      721 gacattcggt tcgcgcagca cccaggcgag ggcgaactgg gccatggtgc agccggcctc
      781 ggcgacgatc ggcctgagcc gctcgacccg ttcgaggatg tccggcgcca tccagcgctg
      841 gatcgcgctg ctcatgctgt cgctggcggc gcgcgagtcc tcgggcaggg gctgacccgg
      901 ccggtacttg ccggtcagca ccccctgggc cagcggcgac cagacgatct gcgagatccc
      961 attggccgcg cacaggggga tgaccttcgc ctcgggccgg cgccagagca gcgaatactg
     1021 cggctggctg gagacgaact tctccacgcc cggcatggcg agcgcggcgg cgatctggtc
     1081 gggcgaccat tccgagaagc cgatgtagcg caccttcccc gcccggacga tctcggtcag
     1141 ggcctgcatg gtctcctcca gcggcgtgtc cgggtcgtag cgatggcact ggtagagatc
     1201 gacgtaatcg gtgcgcaacc gggccagcga cgcgtcgatc tgcttgaaca cctgggcgcg
     1261 ggacaggccc ctgtcggtgt cgctcatcgg ccccatcagc ttggtggcca ggatgtagct
     1321 gtcgcgcggc cggccggcga gcgcctcgcc cagcaccgtc tcggcggcgc cccggccgta
     1381 gacgttggcc gtgtcgagga aggtgatccc caggtcgaag gcgcggttga cgcaggcgat
     1441 ggcgttgtcg cgctcgacgc ccacgccgta ggtcagccac gaccccaggg atatctccga
     1501 gaccatcagg tcgctgctgc cgagctggcg gtatttcatg ggagtctccg aaggtcagcg
     1561 tttggtggcg accagaatgg cgccgagggc gatgagggcc acgcccagcc agttcttgcc
     1621 ggtgagcttt tccccgagga acagggtggc gaagatggcc accagcacga tgctcagctt
     1681 gtcgatcggc gcgacctggg cggccgggcc gagcttgagg gcgcggtagt agcagagcca
     1741 ggacgcgccc gtggccaggg ccgacagggt caggaagatc cagctccggc tcgccacgct
     1801 ggacagcggc tgccaggccc cggtggcggc cacgatgccg gcggtcaccg ccaggatcac
     1861 cgccgtgcgg atcagggtcg cgaagtcgga attgatcgcc tcgaccccga ccttgccgaa
     1921 gatcgccgtc aaggccgcga aggccgccga cagcagcgcc caggcctgcc agggaaggac
     1981 cgcggaactc atcctcccag catcggcggg cgagggcggt caacgcaagc gggaagcgtc
     2041 aggggaatag tttgatggtc gccgtcgcga gcatgatcac gcccgacaga aggccgatga
     2101 tgccccacat ttgctggtct gatcgcctct ggacccgcaa ctccagctgg ccaagtccga
     2161 gcttcgcttc agcggcctgg acaacgaggt cgcgctcgat cgccgcctgg agcgctgcga
     2221 gatcggactt tgtcgccatg tccgcgatgt ccgctttcgt cgccatctcg gcgatgtctg
     2281 atttcgtcgc catatcggcg atgtccgcct tcgtcgccat ctcggcgatg tccgctttcg
     2341 tcgccatctc ggcgatgtcc gctttcgtcg ccatctcggc gatgtccgcc ttcgtcgcca
     2401 tctcggcgat gtctgatttc gtcgccatat cggcgatgtc tgcccgggtg accatgtccg
     2461 cgatatccgc cctggtcgcc atgccggcga gatcagcctt cgtcgccagc tgaccgaagt
     2521 ccaccgatgc tgtcgcgtgc tggaacacgg ccaccaccgc ctccgcgacg ccctcggcca
     2581 tccccgcctc gcgaaggcgt ttggaggcgc tgagcgtatc gaaggcgaac ggcgtcattc
     2641 gcacaaccta ttcgattgtt cccgctttgt ccactattcc gcgagcgcgg gttcctcgat
     2701 ctccggctcg ccgggctgac ggggcttgtc gtcgacctcg aattcgaagg cgatcttgcc
     2761 gtccttgagc acgacgcgga cgtggccgcc cttggcgagc ttgccgaaca ggatgtcgtc
     2821 ggccaggggc ttcttgatgt gctcctggat cacccgggcc agcggccggg cgccgtagag
     2881 ctcgtcgaag ccgttcttgg ccagccagtc ggcggcctcg tcgctggtct cgatggtcac
     2941 gttgcggtcg gccagctggg cttccagctg cagcacgaac ttctgcacca cctggcggat
     3001 gatgtcctgg gtcagcggct tgaaggccac cacggcgtcg aggcggttgc ggaactcggg
     3061 ggtgaagatg cgcttgatcg cctcttccga ctcgccctcc gccttgccgc ggccgaagcc
     3121 gatggtctga cgctgggcgt cggccgctcc ggcgttggtg gtcatgatca ggaccacgtt
     3181 gcggaagtcg accttcttgc cattggaatc ggtcagctgg ccgtggtcca tgacctgcag
     3241 caggatgttg tagacgtcgg cgtgggcctt ctcgatctcg tccagcagca ccacggcgtg
     3301 ggggtgctgg tcgacggcgt cggtcagctg gccgccctgg tcatagccga catagcccgg
     3361 aggggcgccg atcaggcggc tgaccgtgtg tcgctccatg tactcggaca tgtcgaaccg
     3421 cagcagttcg atgccgagcg tcgaggccag ctggcgggcc gcctcggtct tgcccacgcc
     3481 ggttgggccg ctgaacagat agcagccgat cggcttctgc gggtctcgca ggccggcccg
     3541 ggccatcttc atggcgctgg acagctggac gatggcttcg ggctggccga acaccgtgcg
     3601 ctgcaggtcg ttctctagct ccttgagggc ctcggtgtcc gacttgctga ccgacttggg
     3661 cggaatgcgg gcgatcttgg ccacgacggc ctcgatctcc ttcacgccga tggtcttctt
     3721 gcgcttgctt tcgggcagca gcatctggcc ggcgccggcc tcgtcgatca cgtcgatcgc
     3781 cttgtccggc agcttgcggt cggtgatgta cttggccgac aggtccaccg cgaccttgag
     3841 cgcgtcgttg gtgtacttca gcttgtggaa ttcctcgtag tagaccttca ggcccttgag
     3901 gatcttcagg gtgtcctcga tcgtcggctc gttgacgtcg atcttctgga accggcgcac
     3961 cagcgcccgg tccttctcga agtgctggcg gaattccttg taggtggtcg agcccatgca
     4021 gcgcaggctg cccgaggcca gggccggctt gagcaggttg gaggcgtcca tggccccgcc
     4081 ggacgtggcc ccggcgccga tcaccgtatg gatctcgtcg atgaacagga tggcgttggg
     4141 gtggttctcc agctccttga ccacctgctt gacccgctcc tcgaagtcgc cccggtagcg
     4201 ggtgccggcc agcagggcgc ccatgtcgag cgcgaagatg gtcgcgccct caagcacttc
     4261 cggcacctgg tgggtgacga tcttgcgggc caggccctcg gcgatggcgg tcttgccgac
     4321 gcccgggtcg cccaccagca ggggattgtt cttggtccgc cggcagagga tctggatgca
     4381 gcgctcgacc tcgttgtggc ggccgatcag cggatcgacc ttgccctggc gcgccttctc
     4441 gttgaggtcg acgcaatagg cctcgagcgc ctcgccgccg gtcttgaccg aggccttctc
     4501 ctcgtcgccc tcggtgttgg cccccttggc ctgcttgggc tccgaggcgc cggccttctt
     4561 ggcgatgcca tgggcgatgt agttgaccgc gtcgtaccgc gtcatgtcct gctcctgcag
     4621 gaaataggcg gcatggctct cgcgctcgga gaagaccgcg accagcacat tggccccggt
     4681 cacctcgtcg cggcccgagg actgcacgtg gatcacggcg cgctggatca cgcgctggaa
     4741 gccggcggtc ggcttggcgt cctcgccatc ctcgacgatc aggctgcgca gctcggtgtc
     4801 gaggtagttg accagcgtct tcttgagcgc cgccaggtcg acgtcgcagg cggtcatcac
     4861 ccccgcggcg tcctcgtcgt cggtgaggga caggagcaga tgctccaggg tcgcatactc
     4921 gtgctttcgc tggttggcga aggccacggc gcggtggagg gtctcttcga gattgcgcga
     4981 aaacgagggc aagctgggct cctcaatcct tttccattgt gcactgcaac ggatgctggt
     5041 gacggcgggc ggaatcgatc acctgggcca ctttcgtttc agccacttcg taggtgtaga
     5101 ctccacagac gccgaccccg ttctggtgca cgtgaagcat gatgcgggtc gcatcttcgc
     5161 gcgatttgtt gaaaaaccgc tcaagcacgt aaacgacgaa ctccatggga gtgtaatcgt
     5221 cgttgagaat aagcacgcga tagagggacg gcttggcggt cttcggcttg gtctgggtga
     5281 ccaccgccga gccagtgccg ttcgatccag cgcctgattt cttctccgcc attcgtcgtc
     5341 catccagggc gcgcccggga agggctcgcc tcatggccaa ttagatatgg aaacggcgac
     5401 gctttggaag ggccgaagac cccgtgcgcg tcggtttggc gcggacgtga tcaggtttcg
     5461 cgctccgggc gaggtcgcgg gcgcggttcg ccccgtcgcg acggccgtcc gccggtcgtc
     5521 cggcccgaac tttcaacgtc ccgtcgccag gtgagtcgcg gtcctgggga tcagtcccgc
     5581 acctgacggc cgagcgcgcg gcccgtgacc acggcgccct tgccgaaccg ggcccgcagg
     5641 gcgtcgatgg ccgtctcgct cttgaggctg cgtttttccg catccgcgaa gaagtcgcct
     5701 tcggccgccg ccgcctcgac gaaatccgac agacccgcgc cgatcagccg atatgatcgt
     5761 cccgccggct cggccgccag cagctcgcgg gcgacggtga acaatgtccg cgcggtctgg
     5821 gtcgggacgg gcagggtccg gcggcgggta tggatcttga aatccggcgt acgcagcttg
     5881 agcgtcgcca cccgtccggc gatcccctcg gcgcgcagct ggcgagcgac cttttcgcac
     5941 aacggccaca gccggttctc gaggtcctcg acctgatgca ggtcgtcgaa aaaggtggtc
     6001 tcggcgctga ttgtcttgcg gtgctggtcg ggattgacca cccggctgtc ctggcccagc
     6061 gacagcttgt gcagatgcag gccgtgcgat ccgaaccggt cggccagttc gcgcagatcg
     6121 gcggcggcga tgtcgccgac cgtcctgaag ccggccttct ccagcgcggc ggccatgacg
     6181 ggccccaccc cgggcaggat gcgcaccgac cgcggggcca ggaacgcctg ggccccggcc
     6241 gccccgacca ccgagaaacc gcgtggcttg tcgatttccg aggcgatctt ggccatgaat
     6301 ttgttggccg ccaggccgat cgagacggtc aggccgatct cggcctcgat ctcggcctgc
     6361 agcctcgcca gcatcacggc cggtggcgcg ccgtgcagtc tctcggtgcc tgacaggtcg
     6421 atccaggcct cgtccagcga cagcggctgg accagcggcg tcaggtcgcg gaccttgtcg
     6481 aagatgcgct tgctctcggc ccggtacctg gcgaactcgg gcggaaggac gaccgcctgg
     6541 gggcacagct gccgcgcctt gaacatcggc atggccgagc gcacgccgct catgcgggcg
     6601 atgtagcagc aggtggtcac cacaccgcgc ttgccgccgc cgacgatcaa cggcaggtcg
     6661 cgcagctcgg gccggtcgcg cttctcaacc gaggcgtaga aggcgtcgca gtcgaggtgg
     6721 gccatcgaca gggtcgcgag ctcgtcatgg ctcaccaccc gcggcgagct gcaggacggg
     6781 cagcggcgcg gcgcggtgtc cccggtccag aagcagtcgc ggcagagggc cttcatgtgg
     6841 ccagcataca cggccggaga tcgcgcggcg tcaggatgtc ttgacctcgc ggaccgtccg
     6901 gcgtgactag gcgggtcggg aaagaggtgg gtcaatgagc gctgtccggt catcggaaat
     6961 ccgtccggag ccgtccggag agggcctcgg cctgccgggc tggatctatc acgacccgga
     7021 cttcttcgag gccgagaagc gggccgtgtt ccgccagagc tggcagatcg tctgccatct
     7081 gtccgacatc ccgcaggccg gtgacttcca taccctcgaa ttcctcggcg agagcatcgt
     7141 cgtcgtgcgc ggcgacgacc agaaggtgcg cgccttccac aacgtctgcc gccaccgcgc
     7201 ctcgcgcgtc gtcgacggcc cggccggcaa ctgcggcctg cgcatgacct gcccctatca
     7261 cgcctggagc tacgacctgc gcgggcggct cgcgggtgtg ccgcatgtga aggctttcga
     7321 gggcttcgac caggcggcgc acaacctcgc gccggtcgat atcgaggtct gcctgggctt
     7381 cgtgttcatc cggctcgagg gcggcctgcc gtcggtgcgc gagatgctcg gcccctacta
     7441 cgccgagatg gagccctacc gcatggagga cctccagccg ctcggccggg tcacgctgcg
     7501 cgagcgcgag gtgaactgga agaacgtctc ggacaactat tccgacagcc tgcacatcac
     7561 cgtcgcccac ccgggactga cacggctgtt cggagccggc tacggcctcg aggccggcga
     7621 gtggtgcgac aagatgtggg gcgccctgcg cgacacgccg tcggacaact ggtcggagcg
     7681 gcgttaccag gccttcctgc cggtggtcga gcacctgccg ccggagcggc ggcggctgtg
     7741 gacctatttc aagctctggc ccaactgcgc cttcgacatc tatcccgacc agatcgactt
     7801 catgcagttc ctgcccgtct cgccgaccag gactgttttg cgcgagatcg cctacgtcct
     7861 gcccgacgac cgtcgggaaa tgtgcgcgac ccgctacctg aactggcgcg tgaaccggca
     7921 ggtcaacatc gaggacaagg cgctgatcga gcgggtccag gccggcatgg cctcgtccag
     7981 ctacgccatg gggccgctca gcgtgaacga ggtggcgctg aggagcttca cgaagcgcat
     8041 gcgcgagctg atcccgcaga gccgggagcc ggtggcgccc gcgccggggt gggggcgccg
     8101 gtagttcttc ctctccctct tgggagaggg ggaccacccg aagggtggtg gagcgggagg
     8161 cgcgactgtc gacgttaccc gtcgcccgac ccttccgcca ccgcgcatcc ccttccacca
     8221 tgcttcgcat ggtccccctt ccccaagagg ggaaggatca ggaatttagc ggccacagcc
     8281 atgccgcccc gcggacgccg gaggagtccc cgtgcttcgc ctggcggatc ggagtctcga
     8341 aggtgtctga gaagatgtag tgggcgacca cgggcggcac gcgctcgtac agctcgtcga
     8401 cgttggacat gccgccgccc agcacgatga cgtccgggtc gagcaggttg cacagcgagg
     8461 ccagggcccg gcccagtcgg tcgacgtagc gatcgagcgc ctcggcggcg gcggtctccc
     8521 cggcgcgcgc cgcggccacg atggcggctc cgtcacgggt ccggccagag gcctcgcgat
     8581 agtcggcgac gaaggccgag cccgaggtcc aggtctccag gcagtcgacg cggccgcacc
     8641 agcagcggtg cgcctggtgc tcgctcttcg atgaccaggg cagggggctg tggccccatt
     8701 cgccgccgac gccgttgcgc ccctcgaggg tccggccatc gaccaccaca ccgccgccgc
     8761 agccggtgcc gagaatcgcg ccgaacacca cccgggctcc ggccgccgcg ccgtccgtgg
     8821 cctctgacag ggcgaagcag ttggcgtcat tctccagccg cacgggctgg ccaagaacgc
     8881 gcgccagatc ctcgcgcatc ggccggccgt tcatccatac cgaattggcg ttgcgcatca
     8941 ggccggtctt cggcgagatc gagccgggcg cgccgcagcc gacgctggcg ccccggacgc
     9001 ccgcctgccg gccggcttcc agcacgacat cgcgcacgac ctcgaggaag gcttcatagc
     9061 cgcccggatt gggctgtcgc acccgggcga cgaacgatcc gtcggcggcc agggcggcgg
     9121 cctcgacctt ggtgccgccg atgtcgacgc cgatgcggat catcggatcg ccgcgcccag
     9181 cgccggtccc agggtcgacc agtcctcatg caggtcatgc cggtcaggaa cggtcggggc
     9241 atacggccgc agccggtggt cggcgatgat ctggaaggtg gccacggccg gggccgattc
     9301 ggccgaggac tcgagattgg gcaacaggtc gtcgatgaag gccgccggcc cgctcgtgcg
     9361 gccggccatg gcggcgatgg ccgggccctt gaggccggaa tggaggatca tcgggtagtc
     9421 gaacccgtgc ttgcccagcc acccggtgcg cgattcgagc gcatgggccg gggcgttggt
     9481 caggatcacc acgccggccc gttgcgacag gctggccagc gcgtcggccg ccccctcgac
     9541 cgggtcgaga tcgtccgccc catgacggaa gaagtcgtcg aacagggcct tgcctgtctc
     9601 gaagtccagc ggctcggtct cgccgggccg gtacaggttc tggaacaggg cgaagcggtc
     9661 gatgcgcatt tcaacgccgt gccgcgccac gtagcgttcg aacccggcca tgaagtgggc
     9721 cagcacctcg tcgacgtcga cgatgagcag gggcgctgtc ggccggatga ccagatcttc
     9781 gtgggcgacg ggctgcaagg ctgtctcgct gggttagctc gggtttaagg atattcaccg
     9841 attactggca ggaacacggc ggcggcgagt ctcttgttcg tgcgcgacgt cgaaacggct
     9901 ggggttgtgg gtccggatgg ccaagaaggt cctcatcgtc gaggataacg agctcaacat
     9961 gaagctcttc cacgacctgc tcgatgccca gggctacgag acgttgcaga cccgcgaggg
    10021 cctccaggcg ctggcgctgg cccgcgagca ccgcccggac ctgattctga tggacatcca
    10081 gctgcccgag atttccggcc tggaagtcac caagtggctg aaggaagacg acgaactgag
    10141 ccatattccc gtcgtggcgg tcaccgcatt cgcgatgaag ggcgacgagg aacgcatccg
    10201 cgaaggcggc tgcgaggcct atatttccaa gccgatctcg gtgtctcact ttctcgacac
    10261 catccggcgg ttcctggact aggagggcgg aacgatgagc gcgcggatcc tggtcgtcga
    10321 cgacatcgaa gccaatgtga agctgctcga ggccaagctg gcggccgagt actacgaggt
    10381 gctgacggcc agcgacgggc cgacggccct ggccgtcgcc gccgccgaaa gccccgacat
    10441 catcctgctc gatgtgatga tgcccggcat ggacgggttc gccgtctgcc gtcggctnnn
    10501 nnnnnnnnnn nnnntccggt ggtgctggtc accgccctcg acggccggtc ggaccggatc
    10561 gaggggctgg aggccggcgc cgacgagttc ctgaccaagc cgatcgacga tgtgatgctg
    10621 ttcgcccgcg tgcgcagcct gacgcggctc aagcaggtga tggacgaact gcgcgcgcgc
    10681 gaggcgtccg gtcgccgcat cggcgtgatc gccggcgccg cggcccgcct cggcgggtcc
    10741 ggcggccgcg tgctggtggt cgacgacaac gaacgccagg cgcagcggct gacggccgaa
    10801 ctggcgatag agcaccggcc ggtgatcgag accgacccgg agaaggcgca cctgaccgct
    10861 cgcggcccgg tcgacctgat catcgtcaac gccaccgccc gcagcttcga cggcctgcgc
    10921 ttcgcggcca acatccgttc ggacgaggcc acgcgccacc tgccgatcct ggccatcgtc
    10981 gatccggacg agcgccaccg gctgatcaag gcgctcgaga tcggcgtcaa cgacctgctg
    11041 accaagccgc tcgatccaca ggagatggcc gcccgcgccc gcacccagat ccggcgcaag
    11101 cgctacaccg acttcctgcg cgacaatctc gaccattcgc tggagctggc ggtcaccgac
    11161 cagctgaccg gcctgcataa ccgtcgctac atggagggcc agctcggggc cctcgtgaag
    11221 cgggcctcgc atggcggcga cccggtctcg gcgctgatga tcgacatcga ccacttcaag
    11281 aagatcaatg acgccttcgg ccatgacatc ggcgacgagg tgctgcggga attcgcggtg
    11341 cggctcgcgt ccaatgtgcg ggccatcgat ctgccctgcc gctacggcgg cgaggagttc
    11401 accgtgatca tgcccggcgc ccggctggaa gacgcccagc gggtggccga gcgtatccgg
    11461 ctacacgtgg ccggctcgcc gttccgcgtc gccggcggca aggaattgct gacggtgacc
    11521 atctcggtcg gcgtggcctc caccctgggc gaggcggaca ctcccgacgc cctgctcaag
    11581 cgggcggacg aggcggtcta cgaggccaag gcggccgggc gcaaccgggt catcgcgcgc
    11641 gccgcctgaa cgcagaaaac ccggcgcaag gccgggtttc cttcaacata gacaggatgt
    11701 tggcagatct acttgatctt gccttccttg aattcgacgt gcttgcggat gaccgggtcg
    11761 tacttcttca gcacgagctt ctcggtcatg gtgcgggcgt tcttcttggc gacgtagaag
    11821 aagccggtgt ccgccgagga gttcaggcgg atcttgatgg aagccggttt ggccatcgtg
    11881 ggaatccttg ggtagccgcg aagggccgaa acgagaggcg cggaacatac tcatcgcggc
    11941 cgacaagtca atcagggcag ggcgcgcacc agggtcgtcc cgccgcgata ggtcatcatg
    12001 aagggcgatc cgtccgtctg gaggctgcgc agcacggcct cggtgatgtc gtacagcggc
    12061 aaggccagcg actcgtcgaa cgagcgccag gccagatcgg ccagctcgcc gctgtcggcc
    12121 gcctcgccac gggccagctc ggcgtcgacg gcgaagaacc gcgcgtggaa ccgcaccggg
    12181 ctgttggtcg gggtgatcgc gcgggcggcc agggtcaggg ggccatgctc atgcggcagg
    12241 accaggccgg tctcctcctc ggtctcgcgc agggcggcgc tggccagggc ggcggccagg
    12301 cgcgggtcgg ccccggtgcg gcg
//
LOCUS       DAORNX010000028        12232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3915_length_12232_cov_7.859161, whole genome shotgun sequence.
ACCESSION   DAORNX010000028 DAORNX010000000
VERSION     DAORNX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_12232_cov_7.859161"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1298
                     /locus_tag="PLO65_02275"
     CDS             129..>1298
                     /locus_tag="PLO65_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD27106.1"
                     /translation="MGDSRNLVAAIERPGRVIRNLFLASLAAATLAACGQSGPSRPPC
                     PAGKVCLQYGNGSEPLTLDPHLSTGTWEDRIQSDVLVGLSQSDPEGKPIPGMAERWDI
                     SPDGLTWTFHLRQAQWSDGVPVTADDFVYSFRRLLDPKTASEYASVFYLVKGAQAVNE
                     GKAPLETLGVTALDARTLRIELNHPAPYLIELAKHHTMYPVPRHVIERWGADWIKPGH
                     FVGNGPYMLTEYRLGNYVKVVKNPRFWDADKVCIDEVYYYPTNDAISAERRVARGELD
                     LNTDIQSNRIAFLRRTMPGYVRTNTYLGVAYLAFNNNPKTGYPPFRDRRVRQALAMAI
                     DSDFIATKLLRGGQLPATTFTPPGVANYAGAQPAYWTAWPLARRQAEARRLLAQAG"
     assembly_gap    1299..1313
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1314..1788
                     /locus_tag="PLO65_02280"
     CDS             <1314..1788
                     /locus_tag="PLO65_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27107.1"
                     /translation="KLEIKHRNTPDPMLFMPAVQADWKEIGVEATLAQEETQIAYADY
                     RARAFQVADAAWIADYNDPTSFLDLQRSFYGPQNYGDYNNPEYDSLMARADQEPDAVK
                     RAALIARAEHIMLEDAPVAPIFYYVNKNLVRPEITGWVDNLLDHHRTRWLCLKPH"
     gene            2084..5026
                     /locus_tag="PLO65_02285"
     CDS             2084..5026
                     /locus_tag="PLO65_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27108.1"
                     /translation="MRLQSTRGRLLASTIICGAALAVSAPAFAQETEVEAVVVTGSRI
                     PQPNLVGTSPVTQVTSEDITGAGVTKVEDLINSLPQAFAAQNSSVSNGASGTATVDLR
                     GLGSVRTLVLIDGRRLPYGSPNDPAADLNQIPGGMVERVEVLTGGASAVYGSDAIAGV
                     VNFIMKKDFEGVQIDAQYSFYQHNNDFDGTGNIRKEIARRALTNPSQFQLPDSNVIDG
                     YGKEITFLIGVNAPDDRGNLTAYATYRNDDAILQANRDYSGCSIGQPNAASGPGVFSC
                     GGSGTAYPGTFTDFATFNYTVDSATGNTFRNFNAALDQYNFGPVNYYQRPDERYSLGA
                     MGHYEINEHFDVFTQLMFTDYATVAQIAPSGDFFSTNTINCGNPLLSATQASQIGCTA
                     PMIAADTPTTMYIARRNVEGGGRQDDLRYQSFRGVVGTRGSIADGWNYDFAAQYSRVQ
                     LSRIYRNDFSVTRLNRAIDVVDVGGTPTCRSVVNGTDPNCVPWDIFTLGNVSQAALDY
                     LQVPGIQTAWTTQQVVTFAVNGDLGSIGVQSPYADRAVQTAFGVEYRREALSSDTDNA
                     FATGDLAGQGGPTIGLSGVTENYDIFAELVAPLVEGRPMAELLSVEAAYRFSDYGSGI
                     NTDTYKIAGDWAPTSDVRFRASFQRAVRAPNVIELFTAQGLGLFDIDGDPCGPVTPGV
                     TQATLAQCVATGVPAANWRTAALTSPAGQYQFLGGGNPNLNPETADTYTYGVVFTPGF
                     IPGFSLSVDYYNIEVQDLISTVGAGNSLEACYADGDPVACARIHRSGAGQIWVGGGYV
                     DDFNVNIGGLETSGVDVVSNYRFNLDTLGMTDMGSLSLNFVGSWLEKSNTDPGAGQAI
                     DECAGKFAGDCGTPKPEWRHRARATWETPWDASISLTWRHIGEVELHNGNPARIDYKF
                     DAENYFDLSGAWEVTDGVALRGGINNLLDDDPQLSSSVGTTGNGNTYPQTYDSFGRYI
                     FMGVTLDF"
     gene            5738..5926
                     /locus_tag="PLO65_02290"
     CDS             5738..5926
                     /locus_tag="PLO65_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27109.1"
                     /translation="MTRPRLSRAAFAALIDQATAENTDLRIRLLEQRALERLARATRA
                     LAWLKARRRTPRSAARRP"
     gene            complement(5985..8003)
                     /locus_tag="PLO65_02295"
     CDS             complement(5985..8003)
                     /locus_tag="PLO65_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD27110.1"
                     /translation="MTPQFRSLCLAAAIAVFGLAFAAPGQAQAPAPTPPSGEDFGREA
                     AIQRVSIAPDGKHIVALVSPDGQNTYVSIWRTDAMDKPPVNLSCGERSQCVGAFFVKS
                     DRIGVRVRQTITSGADRTHLFRFFTTDLQGKVWRNALGTTDQTAGPNAGVVSLLPRDP
                     TNILIQSYEDYNFYKLDLYSGARRKVATGSDKFSGEQVDLQGEIRARQSFDFENGKVY
                     IAQWIRNPANDQWEEHFRWFAADREPKEIVGFSTDPNIVYVRTNAGRDKAAIYEYDIA
                     ARKFLDPIFEIKLFDAAGVIQSDSPDSFGQILGFSYEGEANKDYWVDPKMASIAKGLR
                     QALGITTVPVQWTDIATGEKTRFSTADGADVQITGWSDDMREVIVVKSGPRQPPEYYL
                     LTDGAKLTKLGSSRPWLNTATLGDTRLVQYAARDGLVVPGFLTTPRKEIYGAGPYPTI
                     ILPHGGPWGRDSLSWDPSGWTQYFAARGYAVLQPQFRGSQDWGQKLWRAGDAEWGQKM
                     QDDMDDGAKWLIAQGIAAPDRIAMHGYSFGGYSAMVASVRPGGIYQCAVAGAGVASID
                     RFRELVNQSRIGREFQRPTILGLSPIDHGADVSIPIFLYHGDRDTTVPIGESERFVAA
                     LKANNKPYKYMPIKDMGHQYVVWEPGQAAMVLRAIDDYLRTDCGPGGL"
     gene            8366..11344
                     /locus_tag="PLO65_02300"
     CDS             8366..11344
                     /locus_tag="PLO65_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27111.1"
                     /translation="MLDRKLLLGTTVIAGFTAALAVAAPTVSFAQTTPPAATTAADDD
                     DDTEVEALVVTGSRIKRTEFTSPAPIQVITAESTTLEGLVDTTEILQQSTLASGSFQV
                     NNMLTGFVVDGGPGVNTISLRGLGANRTLVLLNGRRVGPAGVRGTVGPVDLNVIPNSS
                     IERVEILKDGASSIYGSDAVAGVVNIITKSNLDGAELSAYYKAGEEQGGEFLRFNGVW
                     GRTFDRGFFNISGDYSEQKALRRGDRENLTCSADYLQNAATGAPIDYKGLDGKPKCYN
                     LINNVFDYPQFFGGFFQYRDPALAGIYPTTPGTQPSAVAFGPALAAQLIRSARVGFPE
                     SYPYANYDTPLSDRRTVISPVKRSTVFSTFGYDLTPGAELYGEFLFNRRESEQVSVRQ
                     LFPSVVANNPNNAIFHETGINPYGIMRPIIAVPYTFSQTVDYTRGVIGLRGTFDGDFL
                     NNWDWDFYYQFSRSDGEYTGDIIYNDRVVATTATASACDNTLITISNGLPGGNCSTLP
                     AGGIPWYTSRVISGNFTDAERAFLFAKETGTTTYDQNLVEGSLSGDLFTLPAGPVGVA
                     VGFQWRNEEINDTPGATATNYNIWGLTSAGPTRGEDTVKELFGELAIPVFKGLPGVES
                     LDLSLSGRYTDYDSYGSNSTYKAGVNWQVVPALRVRATYGTSFRAPSLYEQNLGDQTG
                     FLGQTSIDPCIHWEDSTDPILQANCAMEVPFGFTGGTNGSALIYSGGGSNLKAEESKA
                     YTLGVIWTPSFIDLSVAVDYFDIEVSDEVTKFGSGNILTQCYSRTSYPTNAFCGMFTR
                     GGAGINYGIIDVHDDYINISSQTNRGIDLTYRYQHEFDLGRLTLDGQFTWQLHDVTEL
                     QVGAGRDDFNGTTNYYDGPDFTGQLNARFDHGDWTFNWATDLIGKGSDTEQFGGDVFG
                     STRYGTAVYYKQYTEFTAYHDISVRKKFDTWTAVAGITNVFDERAPSVSAGQFRIGTA
                     ALNQYDLVGRSLFVNLSKKW"
     gene            11431..>12232
                     /locus_tag="PLO65_02305"
     CDS             11431..>12232
                     /locus_tag="PLO65_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD27112.1"
                     /translation="MTAQTAPLIARARRLYLQGQAREAEGLCRQALQADPRAAEAYAF
                     LAGMAADQRAFGPSVDLFQRALAIDPRNAAWGADLARSLIGLNRQTPALTAALQALAL
                     GPKDATTFDTLGVVFSRIGHQANAALCFRRAHELAPTQAGYLLNLGWAEQYLGEFAAA
                     EQSWRRCIALDPDNDAAIAALVQMVTQGPDDNFIPALERLFQRAAGDADRQLRVGHAL
                     AKTYEDLKDPLAAFEWLARAKAAKARAATGLAAWQADVFSAARATAPRP"
BASE COUNT         2051 a         4370 c         3870 g         1926 t
ORIGIN      
        1 caaccgcctg cagctgggcg gcgcgggcct tgggcgcgtc ggccgcctgg ccgccgccgg
       61 ccatcatcag aaccgccgct ccggcggcca gggcgatgcc ggacttccgg cgcagggcga
      121 gcgatgacat gggcgactcc cgaaaccttg tggccgccat tgaacggccg ggccgcgtga
      181 tccgcaatct cttcctcgca tcgctggccg cggcgaccct ggcggcctgc ggccagtccg
      241 gtccgtcgcg gccgccctgt ccggccggca aggtctgcct gcagtacggc aacggctccg
      301 aaccgctgac gctcgacccg catctgtcga ccggcacctg ggaagaccgc atccagagcg
      361 acgtgctggt cggcctcagc cagtccgacc cggagggcaa gccgattccc ggcatggccg
      421 agcggtggga catcagcccc gacggcctga cctggacctt ccatctgcgc caggcccagt
      481 ggtcggacgg cgtgccggtg acggccgacg acttcgtcta ttcgttccgg cgcctgctcg
      541 atccgaagac ggcttcggaa tacgcctcgg tcttctatct cgtgaagggg gcccaggcgg
      601 tcaacgaggg caaggcgccg ctcgagaccc tcggggtcac cgcgctcgac gcccgcaccc
      661 tgcggatcga gctcaaccat ccggcgccct acctgatcga gctggccaag caccacacca
      721 tgtatccggt gccccggcac gtgatcgaac gctggggcgc ggactggatc aagcccgggc
      781 atttcgtcgg caacggtccc tacatgctga ccgaataccg gctcggcaac tatgtgaagg
      841 tggtcaagaa cccgcgcttc tgggacgccg acaaggtctg catcgacgag gtctactact
      901 acccgaccaa tgacgcgatc tcggccgagc ggcgggtcgc gcgcggcgag ctggacctca
      961 acaccgacat ccagtccaac cgcatcgcct tcctgcgcag gacgatgccc ggctatgtgc
     1021 gcaccaacac ctacctgggc gtggcctacc tggcgttcaa caacaatccg aagaccggct
     1081 acccgccgtt ccgcgaccgc cgggtgcgcc aggccctggc catggccatc gacagcgact
     1141 tcatcgccac caagctgctg cgcggcggcc agctgccggc cacgaccttc accccccccg
     1201 gcgtggccaa ctacgccggc gcccagccgg cctactggac cgcctggccg ctggcgcgcc
     1261 gccaggcgga ggcccgccgc ctgctggcgc aggccggcnn nnnnnnnnnn nnngaagctt
     1321 gagatcaagc accgcaacac gcccgacccg atgctgttca tgcccgccgt gcaggccgac
     1381 tggaaggaga tcggggtcga ggcgaccctg gcccaggagg agacccagat cgcctacgcc
     1441 gactaccggg cgcgggcctt ccaggtggcc gacgcggcct ggatcgccga ctacaacgac
     1501 ccgaccagct tcctcgatct gcagcgctcg ttctacggac cgcagaacta cggcgactac
     1561 aacaacccgg agtatgattc gctgatggcc cgggccgacc aggagccgga cgcggtcaag
     1621 cgggcggcgc tgatcgcccg cgccgagcac atcatgctcg aggacgcgcc ggtggccccg
     1681 atcttctatt atgtgaacaa gaacctcgtc cggcccgaga tcaccggctg ggtcgacaac
     1741 ctgctcgatc atcaccggac gcgatggctg tgcctgaaac ctcactgaga gggcgccgat
     1801 tcgtcccacg ggcgaaaata cgggttggat ttggcgccgg ccggcgtgaa cggcgccaat
     1861 ctccgcctca atagcgccat ccgggcggct cgctgttgcc aaagtgcgac aatgcggcgc
     1921 ccgttacgtg acggtcctgt aatggtcgtt gaccgccgtt tcccgggaga gcaaggatcg
     1981 ggctaaccgg tcgcctcgtg cgcccggagt cctattcgtc cagatttttg agggggcgcg
     2041 ctaccggact tcccgggccc gcgctgaacc ggagggaatt aagttgaggc tccaatccac
     2101 gcgcggtcgc ctgctggcgt ccaccatcat ctgcggcgcg gctcttgccg tgtcggctcc
     2161 cgcgttcgcg caggagactg aagtcgaagc ggtggtcgtc accggttcgc gcattccgca
     2221 acccaacctg gtcggcacca gccccgtgac ccaggtgacg tcggaagaca tcaccggcgc
     2281 cggcgtgacc aaggtcgaag acctgatcaa ctcgctgccg caagccttcg cggcgcagaa
     2341 ctcgtcggtc tccaacggcg cctcgggcac cgccacggtc gacctgcgcg gcctcggctc
     2401 ggtccgcacc ctggtgctga tcgacggccg tcgcctgccc tacggttcgc cgaacgatcc
     2461 cgccgccgac ctcaaccaga tcccgggcgg catggtcgag cgcgttgaag tgctgaccgg
     2521 cggcgcctcg gccgtctacg gttcggacgc catcgccggc gtcgtgaact tcatcatgaa
     2581 gaaggacttc gaaggcgttc agatcgacgc ccagtacagc ttctaccagc acaacaacga
     2641 cttcgacggc accggcaaca tccgcaagga aatcgcccgc cgcgccctga ccaacccctc
     2701 gcagttccag ctgcccgact ccaacgtcat cgacggctac ggcaaggaaa tcaccttcct
     2761 gatcggcgtc aacgcgccgg acgaccgcgg caacctgacc gcctacgcca cctaccgtaa
     2821 cgacgacgcg atcctgcagg cgaaccgcga ctactcgggc tgctcgatcg gccagcccaa
     2881 cgcggcctcg ggcccgggcg tgttcagctg cggcggttcg ggcacggcct atccgggcac
     2941 gttcaccgac ttcgcgacct tcaactacac ggtcgactcg gcgacgggga acaccttccg
     3001 caacttcaac gcggcgctcg accagtacaa cttcggcccg gtcaactact accagcgtcc
     3061 cgacgagcgt tactcgctcg gcgcgatggg ccactacgag atcaacgaac acttcgacgt
     3121 gttcacccag ctgatgttca ccgactacgc gacggtcgcc cagatcgcgc cgtcgggtga
     3181 cttcttcagc accaacacga tcaactgcgg caacccgctg ctgtcggcga cccaggccag
     3241 ccagatcggc tgtaccgcgc cgatgatcgc cgccgacacc ccgacgacga tgtacatcgc
     3301 ccgccggaac gttgaaggcg gcggccgtca ggacgacctg cgctaccagt cgttccgcgg
     3361 cgtggtcggc acccgcggct cgatcgccga cggctggaac tacgacttcg cggcccagta
     3421 cagccgcgtg cagctgtcgc gtatctaccg caacgacttc tcggtgaccc gcctgaaccg
     3481 cgccatcgac gtggtcgacg tcggcggcac cccgacctgc cgttcggtcg tcaacggcac
     3541 cgacccgaac tgcgtgccct gggatatctt caccctcggc aacgtcagcc aggcggcgct
     3601 cgactacctg caggttccgg gcatccagac cgcctggacg acgcagcagg tcgtgacctt
     3661 cgcggtcaac ggcgacctgg gctcgatcgg cgtgcaaagc ccgtacgcgg accgcgccgt
     3721 gcagaccgcc ttcggcgtcg agtatcgtcg tgaagccctg tcgtccgaca cggacaacgc
     3781 cttcgcgacc ggcgacctcg ccggtcaggg cggcccgacg atcggcctca gcggcgtgac
     3841 cgagaactac gacatcttcg ccgaactcgt ggcgcccctg gtcgaaggcc gtccgatggc
     3901 cgaactgctg tcggtcgaag cggcctaccg cttctccgac tacggcagcg gcatcaacac
     3961 cgacacctac aagatcgccg gtgactgggc cccgaccagc gacgtgcgct tccgcgccag
     4021 cttccagcgc gccgtccgcg cgccgaacgt gatcgaactg ttcacggccc agggcctggg
     4081 cctgttcgac atcgacggcg acccctgcgg cccggtgacc cccggcgtga cccaggccac
     4141 gctcgcccag tgcgtcgcca ccggcgtgcc ggcggccaac tggcgcacgg cggcgctgac
     4201 cagcccggct ggccagtacc agttcctcgg cggcggtaac ccgaacctga acccggaaac
     4261 ggcggacacc tacacctacg gcgtggtgtt cacccccggc ttcatcccgg gcttcagcct
     4321 gtcggtggac tactacaaca tcgaagtgca ggacctgatc agcaccgtcg gcgccggcaa
     4381 ctcgctggaa gcctgctacg ctgacggcga ccccgtcgcc tgcgcccgca tccaccgcag
     4441 cggcgccggt cagatctggg tcggcggcgg ctacgtcgac gacttcaacg tcaacatcgg
     4501 tggtctcgag acctcgggcg tggacgtggt gtccaactac cgcttcaacc tcgacaccct
     4561 cggcatgacg gacatgggca gcctgtcgct caacttcgtc ggctcgtggc tcgagaagag
     4621 caacaccgat ccgggcgctg gccaggcgat cgacgaatgc gccggcaagt tcgcgggcga
     4681 ttgcggcacg ccgaagccgg aatggcgtca ccgggctcgc gccacctggg aaaccccctg
     4741 ggatgcgtcg atctcgctca cctggcgcca catcggtgaa gtcgaactcc acaacggcaa
     4801 ccctgcccgg atcgactaca agttcgacgc cgagaactac ttcgacctgt cgggcgcctg
     4861 ggaagtcacg gatggcgtgg cccttcgcgg tggtatcaac aacctcctcg atgacgatcc
     4921 ccagctgtcc tccagcgtcg gcacgaccgg caacggcaac acctacccgc agacgtacga
     4981 ctccttcggt cgctacatct tcatgggtgt gacgctcgac ttctaaggct caggccttcg
     5041 aaagatctgg ggcggcggac ttcggtccgc cgcccttttt ctttgcgccg tccccgatcc
     5101 tctcccccag ggagaggtgg cccggcggag ccgggtcgga gagggaggag ccgctgttcg
     5161 gcgcggtcct ccaggctgac ggcgctgccc tctccgccct gctccgcagg ggccccgtct
     5221 ccttccggga gaggaagagc ggcggtgaaa aatcagacca cctccgccca accctttgaa
     5281 atcgcaagcc tccgcggtcg ttgctcgacc gcgccaaaca atctgtccat cacccctgcg
     5341 gcccgcgcct atccttaagc cgtccccgcc gcacggggag ggcgcacggc cagcgacctt
     5401 gcggcggcgg ggatggagtt cgaacgggaa caggggcgag ggggttactc gcccggtccg
     5461 gggacggcgc cgcacgccgc ccgcccgtcg ggggtcgata ccggctaagc cttcaaacgg
     5521 ctgcctaccg gagctcccgg aaaacgcaac ttcgcggcgc gtgacggtct acccctctgc
     5581 aaagcctacc caaacagaca tccgggcaga ccggagcgcg ccgcaccacc tccccgcctc
     5641 acggggacat gcacttcagt gcgtgtcccc tgtttgaagt cgttctgatg aaggggacac
     5701 gcactgaagt gcatgtcccc gcgtgaagga acaccccatg accagacccc gcctcagccg
     5761 cgccgccttc gcggccctga tcgaccaggc gacggccgaa aacaccgatc tgcggatacg
     5821 cctgctcgag cagcgggctc tggagcggct ggcccgggcg acccgggcgc tggcgtggct
     5881 gaaggcccgg cgccgaacgc ccaggtccgc cgcccgtcgc ccatgaaaaa agggcgggga
     5941 tcgcgccccg cccttcgttc acgtccgccg cgaggcgagg gggcctacag ccctcccggc
     6001 ccgcagtcgg tgcgcaggta gtcgtcgatc gcccgcagca ccatcgccgc ctggcccggc
     6061 tcccagacca cgtactggtg gcccatgtcc ttgatcggca tgtacttgta cggcttgttg
     6121 ttggccttga gggcggcgac gaaccgctcc gattccccga tcggcaccgt ggtgtcgcgg
     6181 tcgccgtgat agaggaagat cgggatcgac acgtccgcgc cgtgatcgat gggcgaaagg
     6241 cccaggatcg tcggacgctg gaattcgcgg ccgatgcggc tctggttgac cagctcgcgg
     6301 aagcggtcga tcgaggcaac gcccgccccg gccacggcgc actggtagat gccgcccggc
     6361 cggaccgacg cgaccatcgc cgagtagccg ccgaaggagt agccgtgcat ggcgatgcgg
     6421 tccggggcgg cgatgccctg ggcgatcagc cacttggcgc cgtcgtccat gtcgtcctgc
     6481 atcttctggc cccactcggc gtcgccggcg cgccagagct tctggcccca gtcctgcgag
     6541 ccgcggaact gcggctgcag cacggcgtag ccgcgggcgg cgaagtactg tgtccagccg
     6601 ctcggatccc acgacaggct gtcacgcccc cacgggccgc cgtgcggcag gatgatggtc
     6661 gggtagggac ccgcgccgta gatctccttg cgcggcgtgg tcaggaagcc gggcacgacc
     6721 aggccgtccc gcgcggcgta ctgcaccagg cgggtgtcgc cgagggtggc ggtgttgagc
     6781 cacggccgcg aggagcccag cttggtcagc ttggccccgt cggtcagcag gtagtactcg
     6841 ggcggctggc gcgggccgga cttgaccacg atcacctcgc gcatgtcgtc ggaccagccg
     6901 gtgatctgca cgtcggcgcc gtcggccgtc gagaagcggg tcttctcgcc ggtggcgatg
     6961 tcggtccact ggacgggcac ggtggtgatg ccgagcgcct gacgcaggcc cttggcgatc
     7021 gaggccatct tcgggtcgac ccagtagtcc ttgttggcct cgccctcata ggagaagccg
     7081 aggatctggc cgaagctgtc ggggctgtcc gactggatca cgccggccgc gtcgaacagc
     7141 ttgatctcga agatggggtc gaggaacttc cgggcggcga tgtcgtattc gtagatcgcg
     7201 gccttgtccc gcccggcgtt ggtgcgcacg tagacgatgt tcgggtcggt gctgaagccg
     7261 acgatctcct tgggctcgcg gtcggcggcg aaccagcgga agtgctcttc ccactggtcg
     7321 ttggccggat tgcggatcca ctgggcgatg tacaccttgc cgttctcgaa atcgaacgac
     7381 tggcgggcgc ggatctcgcc ctgcaggtcg acctgctcgc cgctgaactt gtccgaaccg
     7441 gtggccacct tgcggcgcgc gcccgaatag agatccagct tgtagaagtt atagtcctcg
     7501 tagctctgga tcaggatgtt ggtcgggtcg cgcggcagca gcgagaccac gccggcgttg
     7561 gggccggcgg tctggtcggt ggtgcccagc gcgttgcgcc agaccttgcc ctgcaggtcg
     7621 gtggtgaaga agcggaacag gtgggtccgg tcggcgccgg aggtgatcgt ctggcgcacc
     7681 cgcacgccga ttcggtcgct cttcacgaag aaggcgccga cgcactgcga tcgctcgccg
     7741 cagctgaggt tgaccggcgg cttgtccatc gcgtcggtgc gccagatgga cacgtaggtg
     7801 ttctggccgt cgggcgagac gagcgcgacg atatgcttgc cgtcgggggc gatgctgacg
     7861 cgctggatgg ccgcttcgcg accgaaatcc tcgccgctcg gcggggtggg ggcgggcgcc
     7921 tgggcctgac cgggcgcggc gaaggccagg ccgaagaccg cgatcgcggc ggccaggcaa
     7981 agggaacgga attgcggcgt cactgtgacc cctcgtaagc tggttttcgc tcggccgccc
     8041 ggggaatcaa acgccccgca cgcgacaaat cgacaatcga gcgcggccga caatcggccc
     8101 atcgggcgca atctaaccac taacgcggtt tcgccaagct gatcttgcct ttcgcgcgtt
     8161 gctggcgctc ggggtcggca atgcccgccg cgggggaaac tgtggcggga ttgttacgcc
     8221 gtgggggcta tggggcaaaa atacgtcatc attcttgtcg gaggcttcga agctgctagt
     8281 ttccctgcac cgcgcgggat gtgtgtcccg tgagcggccg cctgtcggcc gttgaatcat
     8341 cgggctactg gggattaaaa accaaatgct ggaccgcaag cttctcctgg gcacgaccgt
     8401 gatcgccggc ttcaccgccg cgctcgccgt cgccgccccc accgtgtcgt tcgctcagac
     8461 gacgcctccc gccgccacga cggccgctga cgacgacgac gacacggaag tcgaagcgct
     8521 ggtggtcacc gggtcgcgca tcaagcggac cgagttcacc agccccgccc cgatccaggt
     8581 gatcaccgcg gagtccacga ccctcgaggg tctggtggac accaccgaaa tcctgcagca
     8641 gtcgaccctc gcgagcggct cgttccaggt caacaacatg ctgaccggct tcgtcgtcga
     8701 cggcggcccg ggcgtgaaca ccatctcgct gcgcggcctc ggcgccaacc gcaccctggt
     8761 gctgctgaac ggtcgccggg tcggtcccgc cggcgtgcgc ggcaccgtcg gccccgtcga
     8821 cctgaacgtc atcccgaact cctcgatcga gcgggtcgag atcctgaagg acggcgcctc
     8881 gtcgatctac ggctcggacg ccgtggccgg cgtggtcaac atcatcacca agtcgaacct
     8941 cgatggcgcc gagctgtcgg cctactacaa ggccggtgaa gagcagggcg gcgagttcct
     9001 gcgcttcaac ggcgtctggg gccgcacctt cgaccgcggc ttcttcaaca tctcgggcga
     9061 ctattccgag cagaaggccc tgcgtcgcgg cgaccgcgag aacctgacct gctcggccga
     9121 ctatctgcag aacgccgcca ccggcgcgcc gatcgactac aagggtctgg acggcaagcc
     9181 caagtgctac aacctgatca acaacgtctt cgactacccg cagttcttcg gcggcttctt
     9241 ccagtaccgt gacccggcgc tggccggcat ctatccgacg accccgggca cccagccgtc
     9301 ggcggtcgcc ttcggtccgg ccctggccgc ccagctgatc cgctcggccc gcgtcggctt
     9361 cccggaatcc tacccctacg ccaactacga cacgcccctg tccgatcgcc gcacggtgat
     9421 cagcccggtc aagcgcagca cggtgttcag caccttcggc tacgacctga ccccgggcgc
     9481 cgagctctac ggcgagttcc tgttcaaccg gcgcgagtcg gaacaggtca gcgtccgtca
     9541 gctgttcccg tcggttgtcg ccaacaaccc gaacaacgcg atcttccatg agaccgggat
     9601 caacccctac ggcatcatgc gtccgatcat cgcggtgccc tacaccttct cgcagacggt
     9661 ggactacacc cgcggcgtga tcggcctgcg cggcaccttc gacggcgact tcctgaacaa
     9721 ctgggactgg gacttctact accagttctc gcgttcggac ggcgaataca ccggcgacat
     9781 catctacaac gaccgcgtgg tcgccacgac cgccacggcc tcggcctgcg acaacaccct
     9841 gatcaccatc tccaacggcc tgccgggcgg caactgctcg accctgccgg ctggcggcat
     9901 tccctggtac acgtcgcgcg tcatttccgg caacttcacc gacgccgagc gcgccttcct
     9961 gttcgccaag gaaaccggca ccaccaccta cgaccagaac ctggtcgaag gcagcctctc
    10021 cggtgacctg ttcaccctgc cggccggtcc ggtcggcgtg gccgtcggct tccagtggcg
    10081 caacgaggag atcaacgaca ctcccggggc gaccgcgacc aactacaaca tctggggcct
    10141 gacctcggct ggcccgacgc gcggcgaaga caccgtcaag gaactgttcg gcgaactggc
    10201 gatccccgtg ttcaagggtc tgccgggcgt tgaaagcctc gatctgtccc tgtcgggccg
    10261 ttacaccgac tacgacagct acggctcgaa cagcacctac aaggccggcg tcaactggca
    10321 ggtcgtgccc gccctgcggg tccgcgccac ctacggcacc tcgttccgcg cgccgtcgct
    10381 gtacgaacag aacctcggcg accagaccgg cttcctcggc cagacctcga tcgacccctg
    10441 catccactgg gaagacagca ccgatccgat cctccaggcc aactgcgcca tggaagtgcc
    10501 gttcggcttc accggcggca ccaacgggtc ggccctgatc tactcgggcg gcggttcgaa
    10561 cctgaaggcc gaagagtcca aggcctacac cctgggcgtg atctggaccc ccagcttcat
    10621 cgacctgagc gtcgcggttg actacttcga catcgaagtg agcgacgagg tgaccaagtt
    10681 cggctcgggc aacatcctga cgcagtgcta ctcgcgtacg tcgtacccga ccaacgcctt
    10741 ctgcggcatg ttcacccgcg gcggcgctgg catcaactac ggcatcatcg acgtccacga
    10801 cgactacatc aacatcagct cgcagacgaa ccgcggcatc gacctgacct accgctacca
    10861 gcacgaattc gacctcggtc gcctgacgct ggatggtcag ttcacctggc agctgcacga
    10921 cgtcaccgaa ctgcaggtcg gcgccggccg tgacgacttc aacggcacga ccaactacta
    10981 cgacggtcct gacttcaccg gtcagctgaa cgcccgcttc gaccacggcg actggacgtt
    11041 caactgggcc accgacctga tcggcaaggg ctccgacacc gagcagttcg gcggcgacgt
    11101 gttcggcagc acccgctacg gcacggccgt ctactacaag cagtacaccg agttcacggc
    11161 ctaccacgac atctcggtcc gcaagaagtt cgacacctgg accgcggtcg cgggtatcac
    11221 gaacgtcttc gacgagcgtg cgccgtcggt ctcggccggt cagttccgca tcggcaccgc
    11281 ggcgctcaac cagtacgacc tggttggccg cagcctcttc gtgaacctgt ccaagaagtg
    11341 gtagtctgat catctcagag acctgattgg ggcggcggag gacactccgc cgcccttttc
    11401 atttggtccg gggacggcta ggcccaagcc atgaccgcac agaccgcacc cctgatcgcc
    11461 agagcccggc gcctctatct ccagggccag gcgcgtgagg cggaggggct ttgccggcag
    11521 gcgctgcagg ccgatccgcg cgcggcggag gcctacgcct tcctggccgg gatggcggcc
    11581 gatcaacgcg ctttcggccc gtcggtcgac ctcttccagc gcgccctggc gatcgacccg
    11641 cgcaacgccg cctggggcgc ggacctggcc cggtccctga tcgggctgaa tcgccagaca
    11701 ccggccctga cggcggccct ccaggccctg gcgctcgggc cgaaggatgc gacaacattc
    11761 gacaccctgg gtgtggtgtt cagccgcatt ggccaccagg ccaatgccgc actctgtttt
    11821 cgccgggccc atgagctggc gccgacccag gccggctacc tgctcaacct gggctgggcc
    11881 gagcagtatc tgggcgagtt tgccgccgcc gaacagagct ggcgtcgctg tatcgccctc
    11941 gacccggaca acgacgcggc catcgccgcc ctcgtgcaga tggtcaccca gggtccggac
    12001 gacaatttca tcccggcgct ggaacggttg ttccagcggg cggccggcga cgccgaccgt
    12061 cagctgcggg tcggtcatgc cctggccaag acctatgagg acctgaagga cccgctcgcg
    12121 gcgtttgaat ggctcgcccg cgccaaggcc gccaaggcga gagcggccac gggcctggcg
    12181 gcgtggcagg cggatgtgtt cagcgcggcc cgggccacag cgccgcggcc cg
//
LOCUS       DAORNX010000029        12230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3917_length_12230_cov_7.258070, whole genome shotgun sequence.
ACCESSION   DAORNX010000029 DAORNX010000000
VERSION     DAORNX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_12230_cov_7.258070"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLO65_02310"
     CDS             <1..730
                     /locus_tag="PLO65_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD27113.1"
                     /translation="DPYYLAPPATPFAEEVRRDPGKLRIGVIPGALDGGEIDADCAAA
                     VADAAKLCASLGHAVEEVTLDLDFAAIREAAGGVIAASVAATLNMVTAQRGRPIEDGE
                     IDLVTRAMASRGSRVTGADYVLGMQTLHAAGRAVARVHERFDVLLSATLGSPPIRKGS
                     LRGDPLDLAGYGPRLFAFMPNTQLFNVTGQPAMTVPLAWSRTGLPIGIQFAAPMGDEA
                     TLFRLAGQLEQARPWAGKRAPFNG"
     gene            complement(797..2215)
                     /locus_tag="PLO65_02315"
     CDS             complement(797..2215)
                     /locus_tag="PLO65_02315"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRD27114.1"
                     /translation="MPLSPAYRPDPKFMALGPEFSDPVAAADFPITVLRHRNQRAAAT
                     VGLDGLTEAEWIAHFGRFAPLPENQPGPLAMRYHGHQFRSYNPDLGDGRGFLFAQLRE
                     AGTGRLLDLATKGSGQTPWSRRGDGRLTLKGGMREVLAATMLEALGVPTSRAFSLIET
                     GEALERNDEPSPTRSAVLVRLSHSHIRFGTFQRQAYFERGDNIARLVDHCIDAYFPGL
                     SDAPDRAVALLEAVVARTARLAARWMSAGFVHGVLNTDNMVVIGESFDYGPWRFLPRN
                     DPNFTAAYFDETGLYSFGRQPEAAFWNLQQLAGCLTLVAESDALIAVLNGFAPAYRDE
                     LRTAMLARLGLKEQGGDADIELVNAAFRALAEGGERLRWEPFFFDWFCGDEGRAMAGP
                     RADLYAGEAFTAFRARLATAQPERPERLDHPYFLGAEPQEMLVEEVEALWAAIAERDD
                     WSPLAAKLERLEAARAAWGLGN"
     gene            2298..3488
                     /gene="nhaA"
                     /locus_tag="PLO65_02320"
     CDS             2298..3488
                     /gene="nhaA"
                     /locus_tag="PLO65_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRD27115.1"
                     /translation="MTRRPTLAFLRTEAASGLILASAALLALVWANSPWRDLYFAFVK
                     APFTLQVGGWEETRSVLKWTKDGLMAVFFFVVGLEIKYEVLKGELSNPRKLAMPVLAA
                     VGGMAAPALVFLAFNLGADGAPGGWPIPTATDIAFALAVLAIAAPGLPASLRVFLLTL
                     AIADDLGAVALIAVLFTKTIHLAPLAAACAGLAVMVWLWRWKTTPPLVFAAAFLAVWA
                     LTLQSGVNTSLAGVAAALTVPIASRRPGGHGPLKSYMEALHPYVAYGVLPFFAFVAAG
                     FAFDAFPTEGLLAPLPLGIAAGLVLGKPLGVFGAAALAAALKLGQRPSGSTWLQLLGV
                     SMLCGVGFTMSLFIGGLAFNDGAGATQVQLGVVAGSLLSALAGVVVLRLARPAKGRTA
                     NDDF"
     gene            3678..6221
                     /locus_tag="PLO65_02325"
     CDS             3678..6221
                     /locus_tag="PLO65_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27116.1"
                     /translation="MRFSQLLRASASAAVLSAVVAGAAYAQETDEASTVDAIIVTATK
                     REQSLQDVPVVVTAVSGQLMQDAGVKDIKDLTILTPGLTVTSTSNETVTTARIRGVGT
                     VGDNPGLESSVGVVIDGVYRPRNGVSFGDLGELQRVEVLKGPQGTLFGKNTSAGVINI
                     LTVAPSFNFGAQGEVTVGDHGARGVSGSVTGSLTPDDILAGRLFFATRQRDGFLDVET
                     GTAGPRTNDEDVNQDFWTIRGQLLYAPTDTLTVRIIGDYTERDESCCLATQLVVGDAA
                     NSRANLINAVRPGSIDTSESPFERKAYANRSTGQYIVDTGWSAQADLDLSDDVTLTSI
                     TAWRNWRAETGQDSDFSAADIAYRPDDGTNFTEFGQVSQEIRLAGTAGRLDWLVGGFY
                     AKETLGSRSVLTYGQDFYAYFDQRVLGNVPALIGLVPGTIHQAGNGSDDRYNQTDTTF
                     ALFTDDTYAITDALKLTVGLRYTNSEKTLLTRYSTTGASCDQAELMYGPLAGLAGVPT
                     ARAIVGGLCLNGQNNDFDALGAFKQNSGEEEFTGTVKLTYKVSDDIMTYASYARGYKA
                     GGFNLDRSSRARITPFGSPLPFNTAINCAVASGCLDFAVNSNTSFAPEFADSYELGVK
                     TKWFNNSLLLNATLFYQDFTDFQLNTFVGTAFIVESIPEVVSKGVDADFVWFTPVEGL
                     AFQGGVTYAETQFGDFDATDLSDPTRFNGVFRLPGSQVSFAPRWSSSVSGTYERDLTD
                     SLVLRTNISAKYTSRYNTGSDLHPVKDQEAFTLVNARIGIGSTDERWTVELWSNNLFD
                     QDYLQVAFNGPFQVDTSNPYNATADANISTYDTFLGAPRTVGVTLRGKF"
     gene            complement(6315..7244)
                     /locus_tag="PLO65_02330"
     CDS             complement(6315..7244)
                     /locus_tag="PLO65_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27117.1"
                     /translation="MLDKAFRAMLDAAAAIEMPPLAQLPAEMVRAGYVAQRAGLDAGA
                     PKDLDVRDLEVAGAAGPLKARLYAPRGAAATTPGLVYFHGGGFVIGNLDTHDDFLRRL
                     CKASGVRILSVDYRLAPEHPFPAAHDDALAATRWAFDHAAEIGFDPARIAVGGDSAGG
                     NLSACMALELRGDPARKLAFQLLLYPATAMNQDTPSRRALATGYVLTAEVMEWFGNHY
                     GAGTHPQAKRAEPVHAADLSGLPPALVVTAGFDPLKDEGKAYAAALNSAGVAAAHVEY
                     PSFVHDFYTLSAICPGVVPAIEETAAALKAALG"
     gene            complement(7249..9126)
                     /locus_tag="PLO65_02335"
     CDS             complement(7249..9126)
                     /locus_tag="PLO65_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD27118.1"
                     /translation="MTPADGAATAAATSARPPFKPLPQKGPDVSVERRADGSILIWSN
                     HAPGEGPRSIAHLLKDRAQAHPDRPWMKQREPGHGPWRQVTYGEALRAAEGIAQWLLD
                     HGLTNADSVMVLSANSIEHALVTLGCYTAGVPVAPISPAYSLISSDHGKLKHCAAVVK
                     PRVVFAQSGTMFEGAFRTLREANPNLVFVTADGAGEGAVPLAELVATKPTEAVEVARE
                     TLGHATVAKFLFTSGSTGMPKGVPQTFGMMAATIAGAEGLRAEEVDPDLIPQSLEWMP
                     WSHISAGNIGFNGVLWSGGTVHLDEGKPIPGLFETTIKNLYEVSPIVFGSAPIAFGML
                     AEAMERDPVLRASFFKNLRYMGYGGATLSNDVNDRLQALAVAETGHRIPLTTMYGATE
                     TQGVTVTHWATEQVGLVGLPVPGVTIKLVPNGTKLEVRVKGPTVTTGYHNDPEKTAAA
                     FDEEGFYKLGDAAKFIDDNDPAQGMIFDGRVTEDFKLDSGTWVSVGTLRPDLVAACSP
                     WVMDAVIAGQDKPFIGALLWPSMAGMQALAADPGPGSPIDKLTATLRERLAAFNATAG
                     GSSRRVGRFVIMTEPPSIDAGEITDKGYVNQRATLERRDALVKAIYATPAGDGVISVQ
                     D"
     gene            complement(9322..10290)
                     /locus_tag="PLO65_02340"
     CDS             complement(9322..10290)
                     /locus_tag="PLO65_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27119.1"
                     /translation="MTPNFVTIPTNGINLRAVVEGSGPLVVLVHGFPESWYSWRHQIA
                     PLAAAGFKVCAIDVRGYGGSDKPGRVEDYSLEQITADVAGVIEHLAAGEKAVLIGHDW
                     GAPIVWNTALTRPDRVGAVAGLSVPYTGVPAVQFMEVIKAAFTDRGRFFYQVYFQDVG
                     PAEAELGSDPRAAIRKFYYAISGDAPDGTWPADKPHGERLLDRLPDPDPFPAWLTTED
                     EDYFTAEFHRSGFFGPLSRYRNHERDFDWLQSFKDRRIEQPSFFIGGERDLVLKMIPG
                     VNPLDFMKPHLPNLQGAHVLPGVGHWTQQEAPEEVTRLLLGWLKGL"
     assembly_gap    10478..10701
                     /estimated_length=224
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10704..11342
                     /locus_tag="PLO65_02345"
     CDS             10704..11342
                     /locus_tag="PLO65_02345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27120.1"
                     /translation="MITHRAPFSLGEGLARRFAPRYGPLAGAIDASPASLAALAALPT
                     DDAGLLTLEADPVPPPPGMRVAQEAVGVQMVADRLIAADSDLAWIDLTDADAPEMLAL
                     ATLTRPGPFAEETHRLGRFIGVKQDLVAMAGERLRLPGHTEVSGVCTHPEHRGRGYAA
                     ALMRAVMARIIADGETPFLHAYASNTAAIALYETLGFRVRREVAVTGLVKVA"
     gene            11413..11604
                     /locus_tag="PLO65_02350"
     CDS             11413..11604
                     /locus_tag="PLO65_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRD27121.1"
                     /translation="MSDEKPPGFEEAPAEFDADYDERSAAADADVAAGRVVPHEEVAK
                     WLRTWGTPDEKPLPRSWRR"
     gene            11592..11864
                     /locus_tag="PLO65_02355"
     CDS             11592..11864
                     /locus_tag="PLO65_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD27122.1"
                     /translation="MAEVTWAFQALLDLEDIRAYVARDRPVAAERLAERIRAAAAALD
                     RYPARGRPISQGRYELTRIRPYLIRYRVTADGVRILEVRHGARKPE"
     gene            complement(11868..>12230)
                     /locus_tag="PLO65_02360"
     CDS             complement(11868..>12230)
                     /locus_tag="PLO65_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HRD27123.1"
                     /translation="DEGYLYIYDRVKDMIVSGGENVYPAEVENALFSHPAVADAAVIG
                     VPDERWGEAVKGLVVLKPGATATPEEIIAHARSRIAGYKAPKSIDFIDALPRNPSGKV
                     LRRELRKPFWEGRERLVG"
BASE COUNT         1791 a         4298 c         4047 g         1870 t
ORIGIN      
        1 cgatccctac tacctcgccc cgccggcgac cccgttcgcg gaggaggtcc gccgcgatcc
       61 gggcaagctg cgcatcggcg tcatccccgg cgccctcgac ggcggcgaga tcgacgcgga
      121 ctgcgcggcc gcggtcgccg acgcggcgaa actgtgcgcg tcgctcggcc acgcggtgga
      181 agaggtcacg ctcgacctgg acttcgccgc cattcgcgag gcggccggcg gcgtgatcgc
      241 cgccagcgtg gccgcgaccc tcaacatggt caccgcccag cgcggccggc cgatcgagga
      301 cggagagatc gacctcgtca cccgcgccat ggcctcgcgc ggatcgcggg tcaccggggc
      361 cgactatgtg ctgggcatgc agaccctgca cgccgccggg cgggccgtgg ccagggtcca
      421 tgagcggttc gacgtgctgc tcagcgcgac gctcggttcg ccgccgatcc gcaagggctc
      481 cctgcgcggg gatccgctgg atctggccgg ctacgggccg cgcctgttcg ccttcatgcc
      541 caatacccag ctgttcaacg tcaccggcca gccggccatg accgtaccgc tggcctggag
      601 ccggacgggc ctgccgatcg gcatccagtt cgcggcccca atgggcgacg aggcgaccct
      661 gttccgcctg gcgggccagc tggaacaggc gcggccgtgg gcggggaaac gggcgccgtt
      721 caacgggtga catttccatt cactgtcatc ccggatcggc cctgcgggct gtccgggatg
      781 gcaatgcaaa gaaggcctag ttgccaaggc cccacgccgc ccgcgccgcc tccagccgct
      841 cgagcttggc cgccaggggg gaccagtcgt cgcgctccgc gatggcggcc cacagggcct
      901 cgacctcctc gaccagcatc tcctgcggct cggcgcccaa gaaatacgga tggtccagcc
      961 gttcggggcg ctcaggctgc gcggtcgcca accgggcgcg gaacgcggtg aaggcctcgc
     1021 cggcgtagag atccgcgcgc ggcccggcca tcgcccggcc ctcgtcgccg cagaaccagt
     1081 caaagaagaa cggctcccag cgcagccgct cgccgccctc ggccagggcc cgaaacgcgg
     1141 cgttgaccag ctcgatgtcg gcgtctccgc cctgctcctt cagccccagc cgcgccagca
     1201 tcgccgtccg cagctcgtcg cggtaggcgg gcgcgaagcc gttgaggacc gcgatcagcg
     1261 cgtccgactc cgcgaccagc gtcaggcagc cggccagttg ctgcaggttc cagaaggccg
     1321 cctccggctg gcgaccgaag ctgtagagac cggtctcgtc gaaataggcg gcggtgaagt
     1381 tcgggtcgtt gcgcggcagg aagcgccagg ggccgtagtc gaagctctcg ccgatgacga
     1441 ccatgttgtc ggtgttgagc acgccatgga cgaagccggc gctcatccag cgggcggcaa
     1501 gccgggccgt gcgcgcgacg accgcctcca gcagggcgac cgcccgatcc ggcgcgtccg
     1561 acagcccggg gaaataggcg tcgatgcagt ggtcgacgag cctggcgata ttgtcgcccc
     1621 gttcgaaata ggcctggcgc tggaaggtcc cgaagcggat gtggctgtgc gacagccgca
     1681 ccagaacggc cgagcgggtc gggctgggct cgtcgttgcg ctccagcgcc tcgcccgtct
     1741 cgatcaggga gaaggcgcgg ctcgtcggca cgcccaacgc ctcgagcatc gtcgccgcca
     1801 gcacctcgcg catgccgccc ttgagcgtca gccggccgtc gccccgccgc gaccagggcg
     1861 tctggcccga gcccttggtg gccagatcca gcagccggcc ggtccccgcc tccctcaact
     1921 gggcgaacag gaagccgcga ccatcgccga gatcggggtt gtagctgcgg aactggtggc
     1981 catggtagcg catggccagc ggcccgggct gattttcggg caggggcgcg aaccggccga
     2041 aatgggcgat ccactccgcc tcggtcaggc cgtccagccc gaccgtcgcg gccgcccgct
     2101 ggttgcggtg gcggagcacg gtgatgggga aatccgccgc cgccaccgga tcggaaaact
     2161 ccggtcccag ggccatgaac ttcgggtccg gacgataggc aggggacagg ggcatccctc
     2221 gcagatgacg tccgccgccg cccggcgcaa gtccggtttt ggttgggcgg cggccggcgc
     2281 cgtgttagcc ggagggcatg acccgcagac ccaccctcgc gtttctcagg accgaggccg
     2341 cctccggtct catcctggcc tccgccgcgc tgctcgccct ggtctgggcc aactcgccct
     2401 ggcgcgacct gtatttcgcc ttcgtcaagg cgcccttcac gctgcaggtc ggcggttggg
     2461 aagagacccg gtcggtcctc aaatggacca aggacgggct gatggcggtg ttcttcttcg
     2521 tcgtcggcct ggagatcaag tacgaggtcc tcaaggggga actgtccaat ccgcgcaagc
     2581 tggccatgcc ggtgctggcc gcggtcggcg gcatggccgc gccggccctg gtgttcctgg
     2641 ccttcaacct cggcgcggac ggcgcgccgg gcgggtggcc catcccgacg gccacggaca
     2701 tcgccttcgc cctggccgtg ctggccatcg ccgccccggg gctgccggcg tccctgcgcg
     2761 tcttcctgct gaccctggcg atcgccgacg accttggcgc ggtggccctg atcgcggtgc
     2821 tgttcaccaa gaccatccac ctggccccgc tggccgccgc ctgcgccggc ctggccgtca
     2881 tggtctggct ctggcgctgg aagaccacgc cgccgctggt cttcgccgcc gcctttctgg
     2941 ccgtctgggc cctgacgctg cagtcggggg tcaacacgtc cctggccggc gtcgccgcgg
     3001 ccctgaccgt gccgatcgcg agccgccgtc cgggcggcca tggtccgctc aagagctaca
     3061 tggaggcgct gcacccctac gtcgcctacg gggtactgcc gttcttcgcg ttcgtggccg
     3121 ccggcttcgc cttcgacgcc ttcccgaccg aagggctgct ggcccccctg ccgctgggca
     3181 tcgccgccgg ccttgtgctg ggcaagccgc tcggcgtgtt cggcgccgcc gccctcgccg
     3241 cggccctgaa gctcggccag cggccgagcg gatcgacctg gctgcagctg ctgggcgtgt
     3301 ccatgctctg cggcgtcggc ttcaccatga gtctgttcat cggcggcctg gcgttcaacg
     3361 acggcgcagg cgccacgcag gtgcagctcg gggtcgtcgc ggggtcgctg ctttcggccc
     3421 tcgcgggggt cgtcgtgctg cgactcgccc ggcccgcgaa gggccgaacg gcgaacgacg
     3481 atttttgaca ccggcgctgt cacattagag acacgagacg gtcattttcc cttttctaca
     3541 agggattgac gcttgcgcag gttttccggc gcaccgtagg tgatcgccgt caactttgac
     3601 ggagccgtca ggctctcatg tgtcgataac cgcgcggctc ctcccccctc ccccgcgcca
     3661 aaaaaccagg accggaaatg cgtttctcac agttgcttcg cgcgtcggct tccgccgccg
     3721 tgctctcggc cgtcgtcgcc ggcgccgcct atgcccagga aaccgatgaa gcgtcgacgg
     3781 tcgacgccat catcgtcacc gccaccaagc gcgaacagag cctccaggac gtgccggtcg
     3841 tggtcaccgc cgtcagcggc cagctgatgc aggacgccgg cgtgaaggac atcaaggatc
     3901 tgacgatcct gacgcccggc ctgacggtca cctcgacctc gaacgaaacc gtcaccaccg
     3961 cccgcatccg cggcgtcggc accgtcggcg acaaccccgg cctggaatcc tcggtcggcg
     4021 tggtgatcga cggcgtctac cgcccgcgca acggcgtgtc gttcggcgac ctgggcgagc
     4081 tgcagcgcgt cgaagtgctg aagggcccgc agggcacgct gttcggcaag aacacctctg
     4141 ccggcgtcat caacatcctg acggtcgccc cgtcgttcaa cttcggcgcc cagggcgaag
     4201 tcaccgtcgg tgatcacggc gcccgcggcg tgtccggctc ggtcaccggc tcgctgacgc
     4261 ccgacgacat cctggcgggc cgcctgttct tcgccacccg ccagcgcgac ggcttcctcg
     4321 acgtcgagac cggcacggcc ggtccgcgga ccaacgacga agacgtcaac caggacttct
     4381 ggaccatccg cggccagctg ctctacgcgc cgaccgacac cctgacggtg cggatcatcg
     4441 gtgactacac cgagcgcgat gaaagctgct gcctggcgac ccagctggtc gtcggcgacg
     4501 cggccaactc ccgcgccaat ctcatcaacg cggttcgtcc cggttcgatc gacaccagcg
     4561 agtcgccatt cgagcgcaag gcctacgcga accgcagcac cggccagtac attgtcgaca
     4621 cgggctggtc ggcccaggcc gacctggacc tgagcgacga cgtcacgctg acctcgatca
     4681 ccgcctggcg caactggcgc gccgagaccg gtcaggactc cgacttcagc gccgccgaca
     4741 tcgcctatcg accggacgac ggcaccaact tcaccgagtt cggtcaggtc tcgcaggaaa
     4801 tccgcctcgc cggaacggcc ggccgcctcg actggctggt cggcggcttc tacgccaagg
     4861 agacgctcgg ctcgcgctcg gtgctgacct acggccagga cttctacgcc tatttcgacc
     4921 agcgcgttct gggcaacgtc ccggccctga tcggcctggt cccgggcacc atccaccagg
     4981 ccggcaatgg ctcggacgac cgctacaacc agaccgacac caccttcgcc ctgttcacgg
     5041 acgacaccta cgcgatcacc gacgccctga agctgacggt cggcctgcgc tacacgaaca
     5101 gcgaaaagac gctgctcacg cgctactcga ccaccggcgc ctcgtgcgac caggctgaac
     5161 tgatgtacgg cccgcttgcc ggcctggccg gcgtgccgac cgcccgcgcc attgtcggcg
     5221 gcctgtgcct caatggccag aacaacgact tcgacgctct cggcgccttc aagcagaact
     5281 cgggcgagga agagttcacc ggaacggtca agctcaccta caaggtcagc gacgacatca
     5341 tgacctacgc gtcctacgcg cggggctaca aggccggcgg cttcaacctc gaccgctcga
     5401 gccgcgcccg gatcaccccg ttcggctcgc cgctgccctt caacacggcg atcaactgcg
     5461 ccgtggcgag cggttgcctc gacttcgcgg tcaactcgaa caccagcttc gcgcccgagt
     5521 tcgccgactc ctacgagctg ggcgtgaaga ccaagtggtt caacaactcg ctgctgctga
     5581 acgccaccct gttctatcag gacttcaccg acttccagct gaacaccttc gtcggcacgg
     5641 ccttcatcgt cgagtcgatc cccgaggtgg tttccaaggg cgtcgacgcc gacttcgtct
     5701 ggttcacccc ggtcgagggc ctcgcgttcc agggcggcgt gacctacgcc gagacccagt
     5761 tcggtgactt cgacgccacc gacctgagcg acccgacccg cttcaacggc gtgttccgcc
     5821 tgcccggttc gcaggtctcg ttcgcgccgc gctggtcgtc gtcggtctcg ggcacctacg
     5881 agcgcgacct gaccgactcg ctggtcctgc ggaccaacat ctcggccaag tacacctcgc
     5941 gctacaacac cggctcggac ctgcacccgg tcaaggacca ggaggccttc accctggtca
     6001 acgcccgcat cggcatcggc tcgaccgacg agcgctggac ggtggaactg tggtcgaaca
     6061 acctgttcga ccaggactac ctgcaggtgg ccttcaacgg ccccttccag gtcgacacct
     6121 ccaaccccta caacgcgacg gccgacgcca acatctcgac ctacgacacc ttcctgggcg
     6181 cgccccggac ggtcggcgtg accctgcgcg gcaagttcta gtcgcgcaga catcgttgag
     6241 ctgaaaagcg aagggcggct cggggaaacc cgggccgccc ttttctgttt cagcggccgg
     6301 cgacgccggg cgccctagcc gagcgccgcc ttcagcgccg ccgccgtctc ctcgatcgcc
     6361 gggaccacgc ccgggcagat ggccgacagc gtgtagaagt cgtgcacaaa ggacgggtac
     6421 tcgacatgcg ccgccgccac gccggcgctg ttcagcgccg ccgcgtaggc cttgccctca
     6481 tccttcagcg ggtcgaaacc ggcggtgacg accagggccg gcggcaggcc cgacagatcg
     6541 gcggcgtgca cgggctcggc ccgtttcgcc tgcggatggg tccccgcgcc atagtggttg
     6601 ccgaaccact ccatcacctc ggcggtcagc acatagcccg tcgcaagggc gcgccgcgag
     6661 ggggtgtcct ggttcatcgc cgtggccgga tagagcagca gctggaaagc cagcttgcgc
     6721 gccggatcgc cgcgcagctc cagcgccatg caggccgaca ggttgccgcc cgcgctgtcg
     6781 ccgccaacag cgatgcgcgc cggatcgaag ccgatctccg cggcgtggtc gaaggcccac
     6841 cgggtcgcgg cgagggcgtc gtcgtgagcc gccgggaacg gatgctcggg cgccaggcgg
     6901 tagtcgaccg acaggatccg tacgcccgac gccttgcaca gccggcgcag gaagtcgtcg
     6961 tgggtgtcga ggttgccgat gacgaagccg ccgccgtgga aatagaccag tcccggcgtc
     7021 gtggcggccg cgcccctggg tgcatagagc cgcgccttca gcgggccggc ggcgccggcg
     7081 acctcgaggt cgcgcacgtc gagatctttc ggcgcgcccg catcgaggcc cgcgcgctgc
     7141 gcgacatagc cggcgcggac catctcggcc ggcaactggg cgaggggcgg catctcgatg
     7201 gccgcggcgg cgtcgagcat ggcgcggaag gctttgtcga gcatcggatc agtcctggac
     7261 agagatgacg ccgtcgccgg ccggcgtggc gtagatggct ttcacaagcg cgtcgcggcg
     7321 ctcgagcgtg gcgcgctggt tgacgtagcc cttgtcggtg atctcgcccg cgtcgatcga
     7381 cggtggctcg gtcatgatca cgaaccggcc gacccgccgc gaggagccgc cggccgttgc
     7441 gttgaaggcg gccagccgtt ccctgagggt cgccgtcagc ttgtcgatcg gcgagccggg
     7501 ccccgggtcg gccgccaggg cctgcatccc ggccatcgac ggccaaagca gggcgccgat
     7561 gaacggcttg tcctgaccgg cgatgaccgc atccatgacc caggggctac aggccgcgac
     7621 aagatcgggc cgcagggtgc cgacgctgac ccaggtgccg ctgtcgagct tgaagtcctc
     7681 ggtcacccgg ccgtcgaaga tcatgccctg ggcggggtcg ttgtcgtcga tgaacttcgc
     7741 cgcgtcgccg agcttgtaga agccttcctc gtcgaaggcc gcggcggtct tctcgggatc
     7801 gttgtgatag ccggtggtga ccgtcggccc cttgacccgc acctcgagct tggtcccgtt
     7861 cggcaccagc ttgatggtca cccccggcac cggcaggccg acaaggccga cctgctcggt
     7921 ggcccagtgg gtcaccgtca cgccctgggt ctcggtcgcg ccatacatgg tggtcagcgg
     7981 gatgcggtgg ccggtctcgg ccacggccag ggcctgcagc cggtcgttga cgtcgttcga
     8041 cagcgtcgcc ccgccatagc ccatgtagcg caggttcttg aagaacgagg cccgcaggac
     8101 cgggtcccgc tccatggcct cggccagcat gccgaaggcg atcggcgccg agccgaacac
     8161 gatgggcgag acctcgtaga ggttcttgat cgtggtctcg aacaggcccg ggatcggctt
     8221 gccctcgtcg aggtgcaccg tgccgcccga ccagagcacg ccgttgaagc cgatgttgcc
     8281 ggcgctgata tggctccagg gcatccattc gaggctctgg gggatcaggt ccggatcgac
     8341 ctcctcggcc cgcagcccct cggccccggc gatggtcgcc gccatcatgc cgaaggtctg
     8401 cggcacgccc ttgggcatgc ccgtcgagcc ggaggtgaac aggaacttgg ccacggtcgc
     8461 gtggccgagc gtctcgcgag ccacctcgac ggcctcggtc ggcttcgtcg cgaccagctc
     8521 ggccagcggg acggcgccct ccccggcccc gtcagcggtg acgaagacca ggttggggtt
     8581 ggcttcgcgc agggtcctga aggcgccctc gaacatcgtc cccgactggg cgaagacgac
     8641 cctgggcttc accaccgccg cgcagtgttt gagcttgccg tggtcgctcg agatcaggct
     8701 gtaggccggg ctgatcggcg ccaccggcac gccggccgtg tagcagccga gcgtgaccag
     8761 ggcgtgctcg atggaattgg ccgacagcac catcacgctg tcggcgttgg tcaggccgtg
     8821 atcgagcagc cactgggcga tgccctccgc ggcccggagc gcctctccgt aggtgacctg
     8881 gcgccagggg ccgtggcccg gctcgcgctg cttcatccac ggccggtcgg ggtgggcttg
     8941 ggcgcggtcc ttcagcaggt gggcgatcga gcgcggcccc tcgccgggcg catggttgga
     9001 ccagatcagg atcgagccgt cggcgcgccg ttcgaccgac acgtccggtc ccttctgcgg
     9061 caggggcttg aacggcggcc tggcggacgt cgccgcggcc gtggccgcgc catcagcggg
     9121 cgtcatgggc gtttcctgga gctttgatta tcgttgttcc cttgagacta ttcctgaccc
     9181 taggggagtc aaacgggcgt tgggatggtg cgtccttcga cacggccctt cgggcctgct
     9241 caggatgacg tgcatcggtg aagcccgccc ctccacgtca tggtcagcag cgcgaagcgc
     9301 gtgtcgaacc acgcaaggcc gtcacagccc cttcagccag cccagcagca accgggtaac
     9361 ctcctccggg gcctcctgct gggtccagtg gccgacgccg ggcagcacat gggcgccctg
     9421 caggttcggc aggtgtggct tcatgaagtc cagcggattg acgccgggga tcatcttcag
     9481 caccaggtca cgctcgccgc cgatgaagaa gctcggctgc tcgatccggc ggtccttgaa
     9541 cgactgcagc cagtcgaagt cgcgctcgtg gttgcggtag cggctgagcg gcccgaagaa
     9601 gcccgaccgg tggaactcgg ccgtgaaata gtcctcgtct tccgtcgtca gccaggccgg
     9661 gaacgggtcg gggtcgggca ggcggtcgag caaccgctcg ccgtggggct tgtcagccgg
     9721 ccaggtcccg tccggcgcat cgccgctgat ggcgtagtag aacttgcgga tcgccgcccg
     9781 agggtcgctg cccagctcgg cctcggccgg gccgacgtcc tggaaataga cctggtagaa
     9841 gaaccggccc ctgtcggtga aggcggcctt gatcacctcc atgaactgga ccgccggcac
     9901 gccggtgtag ggcaccgaca gcccggccac cgcccccacg cggtcgggac gggtcagggc
     9961 cgtgttccag acgatcggcg cgccccagtc gtggccgatc agcaccgcct tctcgcccgc
    10021 cgccagatgc tcgatgaccc cggcgacgtc ggcggtgatc tgctcgaggc tgtagtcctc
    10081 gacccggccc ggcttgtccg acccgccata gccgcgcacg tcgatggcgc agaccttgaa
    10141 accggcggcg gccagcggcg cgatctggtg acgccagctg taccaactct cgggaaagcc
    10201 atggacgagg acgaccagcg gcccggatcc ctcgaccacg gcgcgcagat tgatgccgtt
    10261 ggtcgggatg gtgacgaagt tgggcgtcat ggcgtttccc cgcgttttcc cgagccgatc
    10321 atgcccgacg ctgcgtcagg caaccctgcc cggcggcatc aaggcggagg tcgcccttat
    10381 gtcttgacgg ccagtgttgc tccggcgggc ggcctgctca aggaccgctt cgcggccgcg
    10441 cagcggcgac cccgcagggg gcgtccttga ccaggacnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nccctgatca cccacagggc gccgttctcg ctcggcgagg
    10741 gtttggcccg ccggttcgcg ccacggtacg ggccgctggc cggggccatc gacgcctcgc
    10801 ccgcaagcct ggccgcgctg gccgccctgc ccaccgacga cgccggcctg ctgacccttg
    10861 aggccgatcc ggttccgccg ccgcccggca tgcgagtcgc gcaggaggcc gtcggggtgc
    10921 agatggtcgc cgaccggctg atcgccgctg acagcgatct cgcctggatc gacctcaccg
    10981 acgccgacgc gccggaaatg ctggcgctgg cgacgttgac ccggcccggc ccgttcgccg
    11041 aagagaccca tcgcctgggt cggttcatcg gtgtgaaaca ggacctcgtc gccatggccg
    11101 gcgaacgcct gcgcctgccc ggccataccg aggtcagcgg cgtctgcacc catccggaac
    11161 accgcggccg ggggtatgcc gcagccttga tgcgggcggt aatggcgcgg atcatcgcgg
    11221 acggggagac gccgttcctc cacgcctacg ccagcaacac cgccgcgatc gcgctgtacg
    11281 agaccctggg gttccgggtg cggcgggagg tcgcggtgac ggggctggtg aaggtcgcgt
    11341 gacgaatgcc atttatagtt gcaacacatc atgactcttc catgcatgga tcgagcgtgc
    11401 tatgctttta ttatgagcga tgaaaagccc ccaggctttg aagaggcgcc cgccgagttc
    11461 gatgcggact atgacgagcg ctcggccgcc gccgacgccg acgtcgcggc gggccgggtc
    11521 gtgcctcacg aggaggtggc caaatggctg cgcacttggg gcacgccgga tgagaagccg
    11581 ttgccccggt cgtggcggag gtaacctggg cgttccaggc gctcctcgac ctagaggaca
    11641 tccgggccta tgtcgcccgg gaccgtcctg tcgccgccga acgcctcgcc gagcgaatca
    11701 gagcggccgc cgcggcgctc gacaggtatc ccgcgagggg tcgcccgatc agccaagggc
    11761 ggtatgaact gactcgcata cggccatacc tgatccgtta tcgcgtgacg gctgatggcg
    11821 tcagaatcct cgaagtccgc cacggcgcgc gcaagcccga gtgagcccta ccccacgagc
    11881 cgctcccgcc cttcccagaa cggcttcctc agctcgcgcc tgagcacctt gccggacggg
    11941 ttccgcggca gggcgtcgat gaagtcgatg gacttgggcg ccttgtagcc ggcgatcctc
    12001 gagcgggcgt gggcgatgat ctcctcgggc gtggcggtcg cgcccggctt gagcacgacc
    12061 aggcccttga ccgcctcgcc ccagcgctcg tccggcacgc cgatgacggc ggcgtcggcg
    12121 acggcgggat ggctgaacag ggcgttctcg acctcggccg gatagacgtt ctcgccgccg
    12181 ctgacgatca tgtccttcac ccggtcgtag atgtagaggt agccctcgtc
//
LOCUS       DAORNX010000030        12187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3939_length_12187_cov_6.881635, whole genome shotgun sequence.
ACCESSION   DAORNX010000030 DAORNX010000000
VERSION     DAORNX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3939_length_12187_cov_6.881635"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLO65_02365"
     CDS             complement(<1..1399)
                     /locus_tag="PLO65_02365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD27124.1"
                     /translation="MTEFSQLGLSASTLQAVADTGYTTATPIQAQAIPVALAGQDVLG
                     IAQTGTGKTAAFTLPMIDRLSAGRARARMPRALVLAPTRELADQVAGSFEKYAKGTRL
                     SWALLIGGVSMGDQVAALDKGVDVLIATPGRLLDLFERSKILLTGVQIMVVDEADRML
                     DMGFIPDIERIFKLTPPKRQTLFFSATMPPEITRLTQQFLKNPTRIEVARPATTGENI
                     AQFVARIPSSDPKAKRVALRSLIDRSEIKNGIVFCNRKSEVDIVAKSLRTHGYDAAAI
                     HGDLDQATRMRTLDGFRKGELKLLVASDVAARGLDIPDVSHVFNYDVPHHADDYVHRI
                     GRTGRAGRSGETFMIVTPADDRNYDKVIKLTGKSPAEATFEGIDWASLKSEPRSKRDG
                     DKRGPRASAGRDRSRGRPASETSVVSMEAISPAASSDAAEKPARRTRERKVEASVEPI
                     SASSAEAPAPAQDPSP"
     gene            1627..1986
                     /locus_tag="PLO65_02370"
     CDS             1627..1986
                     /locus_tag="PLO65_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27125.1"
                     /translation="MSGLTLGLLAAVTIAASAPAPSLEPAFGNTIITTYPNGKTTRTW
                     LHRDGTYDALRSNGERTGGVWKVKNGDLCITQKRPVYVPLTFCTPIVPGGVGASWSAK
                     GLLGEPVRNTLAAGRQG"
     gene            2072..2428
                     /locus_tag="PLO65_02375"
     CDS             2072..2428
                     /locus_tag="PLO65_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27126.1"
                     /translation="MKLLAKAAVAAAMLMAAGPALAADPMATAYGNTVLITYGDGTQV
                     KLFIDEGGSYTGESPQGQSSGSWAISGGQTCFTQQAPEATPPSCGPTVSKNVGDTWQG
                     TGQGGAPVTITIVQGR"
     gene            complement(2481..3893)
                     /gene="otsA"
                     /locus_tag="PLO65_02380"
     CDS             complement(2481..3893)
                     /gene="otsA"
                     /locus_tag="PLO65_02380"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HRD27127.1"
                     /translation="MSRLIVVSNRVSLSSDVGAGSVGGLAAALAAALRESRGVWFGWS
                     GETTPGYTGQIALEQVGEVTVAKVDLEPQDVDEYYNGYANKTLWPLFHDRIDLTAYER
                     LFDAGYARVNQRFAETLRPLVGPDDLVWVHDYHLAPLARELRARGVHNRIGFFLHIPW
                     PARQLLVTLPGHRRLVEALFQYDLIGFQTTEGVSAFTGYLVNELGLAPPDAEGMVTGF
                     GRTVRIGAFPIGINARDFSDGARSPAGEESYQRMRDSLAGRQMILGVDRLDYSKGLEE
                     RFVAYEQFLGEHEEQLERVFLLQIATLSRDSVEAYQDLRARLDAVAGRVNGRFGTVDW
                     VPLRYVNSAYRQDELAGIYRAARVGLVTPLRDGMNLVAKEYVASQDPADPGVLILSRF
                     AGAAGQMSDALIVNPYDRQDMAEAIQQALTMPIEERRRRWESLMLGVETDDTDAWREA
                     FIKALKGETRHRPRLANDAA"
     gene            complement(3890..4654)
                     /gene="otsB"
                     /locus_tag="PLO65_02385"
     CDS             complement(3890..4654)
                     /gene="otsB"
                     /locus_tag="PLO65_02385"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRD27128.1"
                     /translation="MIEPLAPPPSIEGLASPRLLSLRDVALFLDLDGTLAPIAGAPGL
                     VGPDAARNALLSRLQRWLGGRMVVVSGRPVADIDRILDGTVASVSGNHGRERRTAAGD
                     LIALRADPGLPVVTAMLQSFAAAQRGLMVETKPCSVALHYRGHPPAGAACLDIAHRLA
                     KAHGLTVQEGRMVVELLTPGPDKGDALQLFMQEPAFAGRRPVMVGDDLTDEPAFEAAV
                     ALGGYGVLVGEPRETAARYRLANPDAVLAWLSGSLL"
     gene            complement(4888..6939)
                     /gene="parE"
                     /locus_tag="PLO65_02390"
     CDS             complement(4888..6939)
                     /gene="parE"
                     /locus_tag="PLO65_02390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRD27129.1"
                     /translation="MSAPPVQNSLFDDALNPAPVAPLAQAHEPVAAPPPARTPAGGYT
                     ASDIEVLEGLEPVRKRPGMYIGGTDERALHHLFAEVLDNAMDEAVAGHARLIEVHLDA
                     DGTLTVTDNGRGIPVDPHPKYPGKSALEVIMTVLHSGGKFSGKAYETSGGLHGVGVSV
                     VNALSERVEITSWRDGFEHRQVFSRGKPVGKLEQIGPSRKKGTAVAFLPDPVIFGDDA
                     AFKPARLYRMARSKAYLFRGVQIKWSCDPSRIHDQTPAEATFHFPNGLADFLAERIKS
                     VATVTPEAFSGRIERTGEAGAVEWAVTWTPQGFGEADGFMQSYCNTVPTPEGGTHESG
                     FRAALTRGLKAHAELINEKRAGIITADDVVANAGALISVFIRNPEFQGQTKERLSSSD
                     AQKLVETALRDPFDHWLTASPKAARALLDFVIERAEDRLKRRKDREVARASATRKLRL
                     PGKLADCSANAIDGAELFIVEGDSAGGSAKQARDRKTQAILPLRGKILNVASATGDKL
                     MGNKELSDLMLALGVQGGSKYRDEDLRYDRIVIMTDADVDGAHIASLLITFFYRQMPE
                     MIRAGRLYLALPPLYRISHGGKAVYARDDAHKDELLETQFKGKKPEIGRFKGLGEMMP
                     AQLKETTMDPARRTLARVTLPRDEGHVEELVESLMGRKPEQRFRFIQENAEFAAADVD
                     I"
     gene            complement(7115..8458)
                     /locus_tag="PLO65_02395"
     CDS             complement(7115..8458)
                     /locus_tag="PLO65_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD27130.1"
                     /translation="MKRALIAVATLAIATPAAAETVAIVNARIETAAAAGIIPSGTIV
                     LKDGRIAAVGAGVTAPAGARIIDARGGVVTPGLIAPSSNLTVSEVEAVSSTRDDRSGK
                     LSAGFDISYGVNPASPMIPLARQTGLTRVVATPVLSRGGGGGHKDDSGAEDFAGGGGG
                     EEGDPAMFAGQAAIISLAAGDLDPVVRPRVAVTLDLGEAGARNAGGSRGAALVLVKAA
                     FEDARALARNRNAYDRGESREFGLSRIDLEALIPVVEGRAPLLVRVHRASDILQVLRL
                     AREEKVKVILEGAEEGWLVASQIAAAGVGVILDAQADLPDQFETLGARLDNAARLQAA
                     GVTVAIMGSRDLNNLRQERFNAGLAVANGLPRDQAIATITANVAKMWGMTGVGSLEVG
                     KEADVVLWTGDPLETSTWPQAVFIGGVEQPQSTRAFELRDRYLKPAANGYPPAYQ"
     gene            complement(8455..9915)
                     /locus_tag="PLO65_02400"
     CDS             complement(8455..9915)
                     /locus_tag="PLO65_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRD27131.1"
                     /translation="MTSRLRAALAATATAALLAACATTGTAAPDRPVASSGPAKPVPP
                     GLDPAARPDPFPSTYAPFPAGPTAIRGATVLTATGQQIENGVVLFAGGRIVAVGGPDT
                     AIPQGTTVIDGTGKWVTPGIIDAHSHLGVYPSPGVSSRSDGNEATDPVTANVWAEHSV
                     WPQDPGFNRARAGGVTTLQILPGSANLFGGRSVTLRNVPSVTMQGMKFPGAPYGLKMA
                     CGENPKRVYGGKGRSPSTAMGNVAGYRKAWIDAAEYGRKWDDYRAKVARGEKADPPKR
                     DLGLDTLVGVLRGEILVQNHCYRADEMAVMIDIAKEFGYRITMFHHASEAYKVGPLLA
                     KEGICFATWADWQGFKMESYDGIEANAAIAFRSGACTVIHSDDATITQRLNQEAAIAM
                     AAGNRIGLKITDAEAIAWITLNPARAIGIDGQTGSLVAGKRADVVLWSADPFSIYAQA
                     DKVFIDGALVYDRRDPRYQPRSDFELGQPGEGAFHP"
     gene            10035..10394
                     /locus_tag="PLO65_02405"
     CDS             10035..10394
                     /locus_tag="PLO65_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD27132.1"
                     /translation="MTGETRTEAATENRRAQLLKGLAELALLSLLAERPCYGLEILDR
                     LRRDAGLEVAEGTIYPLLHRLERAGLAASDWRLEADGARPRKYYALTAAGRDELAAQT
                     AEWRRLSAGLGAFLDRR"
     gene            10397..10951
                     /locus_tag="PLO65_02410"
     CDS             10397..10951
                     /locus_tag="PLO65_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27133.1"
                     /translation="MTDPMVADWLRRLKWALSAMPAADRDDIVAEARAHLEERLGAGQ
                     PPVEALAGFGAAEAYARPFLDEMELAAALGSQRSGDLLGAVARRIHRSLVAAAAAAVL
                     LILALVAFTAVSLVVMKLRDPVHAGLWIGPGQQFIGTIDDPTKAREWLGLWLYPLAAT
                     VLAGAWMVGRLVLLWSVRRLARTG"
     gene            10994..11893
                     /locus_tag="PLO65_02415"
     CDS             10994..11893
                     /locus_tag="PLO65_02415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRD27134.1"
                     /translation="MRGRAIALYCLLTYGIAWAMQFAALRATGGDLESDAAGPWLATT
                     MFVPGLVTILFLVFHKPARAGLLWKPTWRMIPMLVAGVAVPTLIAFAVLAVVEVMGWG
                     QSGWFGFGADGVRVAGGPWMLGRGMQAWPLFLANVAVTGAAFAAMNGLVAIGEEFGWR
                     AFLQGHLIDRLGLGRGVVLLGLIWSFWHLPSLLAGYNYPETPVLGALVIFPLELVAVS
                     VFMAWITLSARSFWPAVIAHGAGNSIQEGVIANLKMDTPRLCEDLTTLAVTVLVALAC
                     WAWMARPRWSGRAALATRGGSSA"
     gene            11925..>12187
                     /locus_tag="PLO65_02420"
     CDS             11925..>12187
                     /locus_tag="PLO65_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal domain-containing protein"
                     /protein_id="HRD27135.1"
                     /translation="MIASIRTLSLTALAVLSLAACQADGGQSPSPPARAPVAEGGLCG
                     GFAGFQCQAGLYCQMTPEMQHVADGSGTCRKRPQMCTREYRPV"
BASE COUNT         1749 a         4071 c         4375 g         1992 t
ORIGIN      
        1 tcggcgacgg atcctgggcg ggcgcgggcg cttcggccga ggacgcgctg atcggctcga
       61 ccgaggcctc gaccttgcgc tcccgggtcc ggcgggccgg cttttcggcg gcatccgacg
      121 aagcggccgg ggaaatggcc tccatcgaga cgacggaggt ctccgaggcc ggtcggccgc
      181 ggctgcggtc gcgtccggcc gacgcgcggg gaccgcgctt gtcgccgtcg cgcttgctgc
      241 gcggctcgct cttgaggctg gcccagtcga tgccctcgaa cgtggcttcg gccggtgact
      301 tgcccgtcag cttgatgacc ttgtcgtagt tgcggtcatc tgcaggggtg acgatcatga
      361 aggtctcgcc cgaccggccg gcgcggcccg ttcggccgat ccggtggaca tagtcgtcgg
      421 cgtggtgggg cacgtcgtag ttgaacacgt ggctgacgtc gggaatgtcg aggccgcggg
      481 cggccacgtc cgaggccacg agcagcttga gctcgccctt gcggaaaccg tcgagggtgc
      541 gcatgcgcgt cgcctggtcg agatcgccat ggatcgccgc cgcgtcatag ccgtgcgtgc
      601 gcagcgactt ggcgacgatg tcgacctcgc tcttccggtt gcagaaaacg atgccgttct
      661 tgatctcgct gcgatcgatc aggctgcgca gggcgacgcg cttggccttg gggtcgctgc
      721 tcgggatgcg ggccacgaac tgggcgatgt tctcgcccgt ggtcgcgggc cgggccacct
      781 cgatgcgggt cgggttcttc aggaactgct gcgtcagccg ggtgatctcc ggcggcatgg
      841 tggcgctgaa gaacagcgtc tgacgcttgg gcggggtcag tttgaagatg cgctcgatgt
      901 cggggatgaa gcccatgtcg agcatgcggt cggcctcgtc gacgaccatg atctgcacgc
      961 cggtcagcag gatcttgctg cgttcgaaca ggtcgagcag ccggcctggg gtggcgataa
     1021 gcacgtcgac gcccttgtcg agcgccgcga cctggtcacc catggacacg ccgccgatca
     1081 gcagggccca cgaaaggcgg gtgcccttgg cgtatttctc gaagcttccg gccacctggt
     1141 cagccagttc gcgggtcggc gccagcacca gggcgcgcgg catgcgggcg cgggccctgc
     1201 cggccgacag ccggtcgatc atcggcaggg tgaaggcggc ggtcttgccg gtgccggtct
     1261 gggcgatgcc caggacatcc tggcccgcga gggccacggg aatggcctgg gcctggatcg
     1321 gcgtggccgt ggtgtagccg gtgtcagcca ccgcttgcag tgtggaggcg ctcagcccca
     1381 gttgggaaaa ttcggtcatg tctcacgaaa ggaagatcca gagccgcccg tgtttgggcg
     1441 aacgggcggc gcggattcgc cagacctgct ccgaactcgg gcggatacat aggccttaag
     1501 cccgcaaagt caatcgctgc ttgggtttac cgcaggtgta ggcgctcgtg cggcaggcga
     1561 cggggcttgg caagtcgcgg ccagtgctgt tgattgtcgg tccccgaacg ggagatgcaa
     1621 atgtccgtga gcggattgac ccttggtctg ctggccgccg ttacgatcgc tgcctcggcg
     1681 ccagccccca gccttgagcc ggccttcggc aacaccatca tcaccaccta cccgaacggc
     1741 aagacaacgc ggacctggct gcatcgcgac ggcacctacg acgccctgcg atccaacggc
     1801 gagcgaaccg gcggggtatg gaaggtcaag aacggcgatc tgtgcatcac ccagaagcgg
     1861 ccggtctatg tgcctctgac cttctgcacg ccgatcgtgc ccggcggggt gggggccagc
     1921 tggtctgcga aggggctgct gggcgagccc gtgcgcaata ccctggccgc cggacggcag
     1981 ggttaacgtc ccggaaattc ttcgctaggc tgccgccact cgcctgaagg gcgagatcgc
     2041 agcaacgaag aaaacgggcg gaaacaacaa gatgaaactc cttgcgaagg cggctgtcgc
     2101 cgccgcgatg ctcatggcgg ccggtccggc gctggcggca gatccgatgg ccacggcgta
     2161 cggcaacacc gtcctgatca cctacggcga cggcacgcag gtcaaactct tcatcgacga
     2221 gggcggctcc tataccggcg agagccccca gggccagagc tcgggctcct gggccatctc
     2281 cggcggccag acctgcttca cccagcaggc gccggaggcc actccgccgt cgtgcggccc
     2341 gaccgtcagc aagaacgtgg gcgatacctg gcagggtacg ggccagggcg gcgcgccggt
     2401 gaccatcacc atcgtccagg gtcgctagtc agggcatggt ccggcccggg gtgacggccg
     2461 ccgacagcgg tcgtcgccct tcaggccgcg tcgttggcca gacgcggccg gtgtcgggtc
     2521 tcccccttga gggccttgat gaaggcttcc ctccaggcgt cggtgtcgtc ggtctcgacg
     2581 ccgagcatca gcgattccca gcggcgccgg cgttcctcga tcggcattgt cagcgcctgc
     2641 tggatggcct ccgccatgtc ctggcggtca tagggattga cgatcagggc gtccgacatc
     2701 tggccggccg cgccggcgaa ccgtgacagg atcaggaccc cgggatcggc cggatcctgc
     2761 gaggcgacat actccttggc caccagattc atgccgtccc gcaagggcgt caccaggccc
     2821 acccgcgcgg cgcggtagat gccggccagt tcgtcctggc ggtaggcgct gttgacgtag
     2881 cgcagtggaa cccagtcgac ggtgccgaac cggccgttga cgcggccggc cacggcgtcg
     2941 agccgggcgc gcaggtcctg gtaggcctcg acgctgtcgc ggctgagggt ggcgatctgc
     3001 aacaggaaca cgcgctcgag ctgttcctcg tgttccccga gaaactgctc gtaggcgacg
     3061 aagcgttcct ccaggccctt ggagtagtcc agccggtcga cgcccaggat catctgcctg
     3121 ccggcgaggc tgtcgcgcat gcgctggtag ctctcctcgc cggccgggga ccgggccccg
     3181 tcggaaaagt ctcgcgcgtt gatgccgatc gggaaggcgc cgatgcggac ggtgcggccg
     3241 aagccggtga ccatgccctc ggcgtcaggg ggagccaggc ccagttcgtt gacgaggtag
     3301 ccggtgaagg ccgacacgcc ctcggtcgtc tggaaaccga tcaggtcgta ctggaacagc
     3361 gcctcgacca gcctgcggtg cccgggcagg gtgaccagca gctggcgcgc gggccagggg
     3421 atgtgcagga agaagccgat ccggttgtgg acgccccgcg cccgcagctc gcgcgccagc
     3481 ggcgccaggt ggtagtcgtg cacccagacc agatcgtcgg gcccgaccag cgggcgcagg
     3541 gtctcggcga accgttggtt gacccgggcg tagccggcgt cgaacagccg ttcgtaggcg
     3601 gtcaggtcga tgcggtcgtg gaacagcggc cacagggtct tgttggcgta tccgttgtaa
     3661 tattcgtcga cgtcctgggg ttcgaggtcg accttggcca cggtcacctc gccgacctgc
     3721 tccagcgcga tctggcctgt gtagccgggc gtggtttcgc cgctccagcc gaaccagact
     3781 ccgcgacttt cgcgcagcgc cgccgccagg gccgcggcga ggccacccac agagccggcg
     3841 ccgacgtcgc tcgacaggct cacgcgattg gacaccacga tcagccgact catagcagcg
     3901 accccgaaag ccaggccagc acggcgtcgg gattggccag ccggtaacgg gcggccgtct
     3961 cccgcggttc accgaccagc accccgtagc cccccagggc gacggcggcc tcgaacgccg
     4021 gctcatcggt caggtcgtcg ccgaccatca ccggccggcg gccggcgaag gccggttcct
     4081 gcatgaacag ttgcagcgca tcgcccttgt cgggccccgg cgtgagcagc tccaccacca
     4141 ttctgccctc ctggaccgtc aggccatggg ctttcgccag ccggtgggcg atgtcgaggc
     4201 aggccgctcc cgccggggga tggccgcgat agtgcagggc gacgctgcag ggcttggtct
     4261 cgaccatcag gccgcgctgg gccgccgcga aactctgcag catcgcggtc acgacgggca
     4321 ggccgggatc agccctgagg gcgatcaggt cgcccgccgc ggttcgccgt tcgcggccat
     4381 gattgccgga gacgctggcc accgtcccgt cgagaatccg gtcgatgtcg gccaccggcc
     4441 ggccgctgac cacgaccatc cggccgccca gccagcgctg cagccgcgac agcagggcgt
     4501 tgcgcgccgc gtcgggcccg acaagcccgg gggcgccggc gatcggcgcc agggtgccgt
     4561 cgaggtcgag aaacagtgcg acgtcccgaa gggacagtag tcgtggcgac gccaacccct
     4621 cgatcgaggg gggcggtgcg agcggttcga tcattcgtgc cccagcttgt tggccgtgaa
     4681 acgcgcgaag gcgtccgttt cacgcgaaag cagtctacag gatcaccaaa acgccggggg
     4741 taaacttagg ttcactcaaa gttgaactga aaatcacatc aagtaatgaa tagtcatgat
     4801 catcgcgcaa atacagtact tgcaggtaat ttccgcggat gaaagtttct cgagcccgtc
     4861 agtctcgcgc tgtcgttaca tcaattgtca gatgtcgacg tccgccgccg cgaattcggc
     4921 gttttcctga atgaaccgga agcgctgttc gggcttgcga cccatcaggc tttccaccag
     4981 ttcctccacg tggccctcgt cacgcggaag ggtgacccgg gccagggtgc gccgggccgg
     5041 gtccatggtg gtttccttca gctgggccgg catcatttcg cccaggccct tgaagcggcc
     5101 gatctcgggc ttcttgccct tgaactgcgt ctccagcagt tcgtccttgt gcgcgtcgtc
     5161 acgggcgtag acggccttgc cgccgtggct gatgcgatag agagggggca gggccagata
     5221 cagccgcccg gcgcggatca tctccggcat ctggcggtag aagaaggtga tcagcaggct
     5281 ggcgatgtgg gcgccgtcga cgtcggcgtc ggtcatgatc acgatgcggt cgtagcgcag
     5341 gtcctcgtcg cgatacttgc tgccgccctg cacgcccagc gccagcatca ggtccgacag
     5401 ctccttgttg cccatcagct tgtcgcccgt ggcgctggcg acgttgagga tcttgccgcg
     5461 caggggcagg atcgcctggg tcttgcggtc gcgcgcctgt ttggccgagc cgccggcgct
     5521 gtcaccctcg acgatgaaca gctcggcgcc gtcgatggcg ttggccgagc agtccgccag
     5581 cttgccgggc agccgcagct tgcgggtggc cgaggcgcgc gcgacctcgc ggtccttgcg
     5641 ccgcttgagc cggtcctcgg cgcgttcgat gacgaaatcg agcagggccc gggcggcctt
     5701 gggcgaggcg gtcagccagt ggtcgaacgg atcgcgcagc gcggtctcga ccagcttctg
     5761 ggcgtcggac gagctgagcc gctccttggt ctggccctgg aactccgggt tgcggatgaa
     5821 cacgctgatc agcgccccgg cgttggcgac gacgtcgtcg gccgtgatga tgccggcgcg
     5881 cttctcgttg atcagctcgg catgagcctt gaggccccgg gtcagggcgg cgcggaagcc
     5941 ggattcgtgc gtgccgccct cgggcgtggg cacggtgttg cagtaggact gcatgaagcc
     6001 gtcggcctcg ccgaagccct gcggcgtcca ggtgacggcc cattccacgg cgcccgcctc
     6061 gccggtgcgc tcgatgcggc cgctgaaggc ttcgggcgtg accgtggcaa cgctcttgat
     6121 ccgctcggcc aggaagtcgg ccaggccgtt ggggaagtgg aacgtcgcct cggccggggt
     6181 ctggtcgtga atgcgcgacg ggtcgcagga ccatttgatc tgcacgccgc ggaacagata
     6241 ggccttggag cgggccatgc ggtagagccg cgccggcttg aaggccgcgt cgtcgccgaa
     6301 gatcaccggg tccggcagga aggcgaccgc ggtgcctttc ttgcgcgacg ggccgatctg
     6361 ctcgagcttg ccgaccggct tgccgcgcga gaacacctgg cggtgctcga aaccgtcgcg
     6421 ccaggaggtg atctccaccc gctccgacag ggcgttgacg acactgaccc ccacgccatg
     6481 caggccgccg gaggtctcgt aggccttgcc ggagaatttc ccgccagagt ggaggacggt
     6541 catgatcact tccagcgccg acttgcccgg atacttgggg tgcgggtcga cgggaatgcc
     6601 ccgcccgttg tcggtgacgg tcagggtccc gtcggcgtcc agatggacct cgatcagcct
     6661 ggcgtggccg gcgacggcct cgtccatggc gttgtcgagc acttcggcga acaggtggtg
     6721 cagcgcccgc tcgtcggtgc cgccgatgta catgcccggc cgcttgcgta cgggctccag
     6781 gccctcgagc acctcgatgt cgctggccgt gtagccgccg gcgggggtcc tggccggcgg
     6841 aggcgccgcc accggctcat gggcctgcgc cagcggcgcg accggcgccg ggttcagggc
     6901 gtcgtcgaaa agggagttct gcacaggggg ggcggacatg acgctggcga gtttcgggcg
     6961 attcgggggc gtcgaggtat gcggcgaagg gccgggaggg gcaactccaa tccttctccc
     7021 cttgcgggag acggtgtcgg gcgggcttgt cggcccctca tccgacccgc ttcgcgggcc
     7081 accttctccc gtaaggggag aaggcgttcg ttccctactg atacgccggt ggatacccgt
     7141 tcgcagccgg cttgaggtaa cgatccctga gctcgaaggc ccgcgtgctc tggggctgtt
     7201 cgacgccgcc gatgaacacc gcctgcggcc aggtgctggt ctccagcgga tcgccggtcc
     7261 acagcacgac gtccgcctcc ttgccgactt ccagcgagcc gacgccggtc atgccccaca
     7321 tcttcgccac gttggcggtg atggtcgcga tggcctggtc gcgcggcagg ccgttggcca
     7381 ccgccagtcc ggcgttgaag cgttcctggc gcaggttgtt gaggtcgcgg ctgcccatga
     7441 tcgccacggt caccccggcc gcctgcagcc gggcggcgtt gtcgaggcgg gcgccgaggg
     7501 tctcgaactg gtcgggcagg tcggcctggg cgtcgaggat cacgccgacg ccggcggcgg
     7561 cgatctgcga cgccacgagc cagccctctt cggccccctc gaggatcacc ttgaccttct
     7621 cctcgcgcgc caggcgcagc acctgcagga tgtcgctggc gcggtgcacc cgcaccagca
     7681 gcggcgcacg accctcgacc acagggatca gggcctcgag gtcgatgcgc gacaggccga
     7741 actcgcgact ctcgccgcgg tcataggcgt tgcggttgcg ggcaagggcg cgagcgtcct
     7801 cgaaggccgc cttcaccagc accagcgccg cgccgcggga gccgccggcg ttgcgcgcgc
     7861 cggcctcgcc cagatcgagg gtcacggcca cgcgcggccg gaccaccgga tccaggtcgc
     7921 cggccgccag cgagatgatg gcggcctggc cggcgaacat cgccggatcg ccttcctcac
     7981 cgccgccgcc gcccgcgaag tcctcggccc cgctgtcgtc cttgtggccg ccgccaccgc
     8041 cacgcgagag cacgggcgtc gcgacgacgc gggtcaggcc ggtctgccgc gccagcggga
     8101 tcatcgggct ggcgggattg acgccgtaac tgatgtcgaa gccggccgaa agcttgccgg
     8161 agcgatcgtc acgcgtcgag gacacagcct cgacctcgct gaccgtgagg ttggaggagg
     8221 gggcgatcag gccgggtgtg accactccgc cgcgggcgtc gatgatccgg gcgccggcgg
     8281 gagccgtgac gcccgctccc acggcggcga tgcgaccgtc cttcagcacg atggttccgg
     8341 acgggatgat gcccgccgcc gccgcggtct cgatgcgggc gttgacgatg gccacggtct
     8401 cggccgccgc cggcgtggcg atggccaggg tggcgacagc gatgagggcg cgtttcatgg
     8461 gtggaaggcc ccttcgccgg gctggccgag ttcgaagtcg gacctgggtt ggtaacgtgg
     8521 gtcgcgccgg tcgtagacca gggccccgtc gatgaagacc ttgtcggcct gggcgtagat
     8581 gctgaaggga tcggcgctcc acaacaccac atcggcccgc ttgccggcga ccagcgagcc
     8641 ggtctggccg tcgatgccga tggccctggc cgggttgagg gtgatccagg cgatcgcctc
     8701 ggcgtcggtg atcttcagcc cgatgcggtt gccggccgcc atggcgatgg ccgcctcctg
     8761 gttcaggcgc tgggtgatgg tcgcgtcatc ggagtggatg accgtgcacg cccccgagcg
     8821 gaaggcgatc gcggcgttgg cctcgatgcc gtcgtaggac tccatcttga agccctgcca
     8881 gtcggcccag gtggcgaagc agatgccctc cttcgccagc agaggcccga ccttgtaggc
     8941 ttccgaggcg tggtggaaca tggtgatgcg gtagccaaac tccttggcga tatcgatcat
     9001 cacggccatt tcgtcggccc ggtagcagtg gttctgcacc aggatctcgc cgcgcagcac
     9061 cccgaccagg gtgtcgagac ccagatcgcg cttgggcgga tcggccttct cgcccctggc
     9121 gaccttggcg cggtagtcgt cccacttgcg gccgtattcc gccgcgtcga tccacgcctt
     9181 gcggtagccg gccacgttgc ccatcgccgt ggacggcgaa cggcccttgc cgccgtagac
     9241 ccgcttggga ttttcgccgc aggccatctt caggccatag ggcgccccgg ggaacttcat
     9301 cccctgcatg gtcaccgacg gcacgttgcg cagcgtgacc gagcggccgc cgaacaggtt
     9361 ggccgagccg ggcaggatct gcagggtggt gacgccgccg gcccgggccc ggttgaagcc
     9421 cggatcctgc ggccagaccg aatgttcggc ccagacgttg gccgttaccg gatcggtggc
     9481 ctcgttgccg tccgaccgcg acgagacgcc gggcgaggga tagacgccca ggtggctgtg
     9541 ggcgtcgatg atgcccggcg tgacccactt gccggtcccg tcgatgacgg tcgttccctg
     9601 cgggatggcg gtgtccgggc cgccgacggc gacgatccgg ccgccggcga agagcaccac
     9661 gccgttctcg atctgctggc cggttgccgt cagcaccgtg gcgccgcgga tggcggtcgg
     9721 cccggcgggg aagggggcgt aggtcgacgg gaagggatca ggccgggcgg ccggatcaag
     9781 acccggcggg accggcttgg ccggacccga actggcgacg ggcctgtcgg gcgccgccgt
     9841 gcccgtggtg gcgcaggcgg ccagcaaggc cgccgtcgcg gtggcggcca gcgccgcgcg
     9901 taatctggat gtcatcgccc cgtcccgaac tcgaagacgg cgagatcaga cgaaaagcgc
     9961 cgcccggtca attcgcatcc gggcgaacgc cgtgcgttga ccgccgtgca aaatatgata
    10021 tcttgcatta caagatgact ggcgaaacac gtacagaggc ggcgaccgaa aatcgccgcg
    10081 cgcagctgct caagggcctg gcggagctgg cgctgctcag cctgctggcg gagcgcccct
    10141 gctatggcct ggagattctc gaccgcctgc gccgcgatgc cggcctggag gtggcggagg
    10201 gcaccatcta cccgctgctg cacagactcg agcgggccgg actggcggcg tccgactggc
    10261 ggctggaggc ggacggggcc cgtccgcgca aatactacgc cctgaccgcg gccgggcggg
    10321 acgaacttgc ggcgcagacc gccgagtggc gacggctcag cgcggggctg ggcgcctttc
    10381 tggacaggag atgaccatga cggacccgat ggtggccgac tggttgcggc gtctcaaatg
    10441 ggcgctgtcg gccatgcccg cggccgatcg cgacgacatc gtcgccgagg cgcgggcgca
    10501 tctggaagaa cgtctcggcg ccggacagcc accggtcgag gcgctggccg gatttggcgc
    10561 ggccgaggcc tacgcccgac cgttcctcga cgagatggag ctggccgcgg ccctcggctc
    10621 gcaacggtcg ggtgacctgc tgggcgcggt cgcccgtcgc atccatcgca gcctggtggc
    10681 cgccgccgcc gccgccgtgc tgctgattct ggccctggtc gccttcacgg cggtcagcct
    10741 ggtggtgatg aagctgcgcg accccgttca cgcaggtctg tggatcgggc cggggcaaca
    10801 attcatcggc acgatcgacg atccgaccaa ggcccgtgag tggctcggcc tgtggctcta
    10861 tccgctggcg gcgaccgtgc tggccggcgc ctggatggtc gggcgtctgg tcctgctgtg
    10921 gtccgtgcga cggctcgccc gaaccggctg acggccggcc tccacatcct tccagaaacg
    10981 aaagggtctt cccatgcgcg gacgcgccat agccctctat tgcctgctga cctacggcat
    11041 cgcctgggcg atgcagttcg ccgccctgcg cgccacgggc ggtgatctcg aatcggacgc
    11101 cgccgggcca tggctggcga cgacgatgtt cgtccccgga ctcgtgacga tcctgtttct
    11161 cgtgtttcac aagccggccc gcgccggcct gttgtggaaa ccgacctggc ggatgatccc
    11221 gatgctggtc gccggcgtgg ccgtaccgac cctgatcgcc ttcgccgtgc tcgcggtcgt
    11281 cgaggtcatg ggctggggcc agtcgggctg gttcggcttc ggcgccgacg gcgtgcgtgt
    11341 cgccgggggc ccgtggatgc tcgggcgcgg gatgcaggcc tggccgctgt tcctggccaa
    11401 tgtcgccgtc accggcgcgg ccttcgcggc gatgaacggc ctggtcgcca tcggcgagga
    11461 gttcggctgg cgcgccttcc tgcagggtca tctgatcgac cggctgggcc ttggccgggg
    11521 cgtggtgttg ctgggcctga tctggtcgtt ctggcacctg ccgtcgctgc tggccggcta
    11581 caactacccg gaaacgccgg tgttgggggc gctggtgatc ttccccctcg agctggtggc
    11641 ggtgtcggtg ttcatggcct ggatcacact cagcgcgcgc agcttctggc cggcggtgat
    11701 cgctcacggg gccggcaact cgatccagga aggcgtgatc gccaatctga agatggatac
    11761 gccccggctc tgcgaggacc tgaccaccct ggcggttacg gtgctggtgg ccctggcctg
    11821 ctgggcctgg atggcccgac cgcgatggtc ggggcgggcg gcccttgcaa cccgcggcgg
    11881 gtcgagcgcg taatcagggc ctggccttgc acggagcgcc gccgatgatc gcctcgatcc
    11941 gcaccctctc cctgaccgct ctggcggtgc tgtcgctggc ggcctgccag gctgacggcg
    12001 gtcagtctcc gtcgccgccg gcgcgcgcgc cggtggccga gggcggcctg tgcggaggct
    12061 tcgccggctt ccagtgtcag gccggcctct attgccagat gacgcccgag atgcagcacg
    12121 tcgccgacgg ctcggggacc tgccgcaagc gaccacagat gtgcacccgc gagtaccgtc
    12181 cggtctg
//
LOCUS       DAORNX010000031        12094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_3979_length_12094_cov_7.126506, whole genome shotgun sequence.
ACCESSION   DAORNX010000031 DAORNX010000000
VERSION     DAORNX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_12094_cov_7.126506"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLO65_02425"
     CDS             <1..369
                     /locus_tag="PLO65_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRD27136.1"
                     /translation="RLAMVLAVLESRCGLAFGNKDVYLNVAGGLRITEPAADLAAAAA
                     LASSALDEALPQDCVVFGEVSLSGEVRPVGRAETRLKEAAKLGFRRALAPIGAGESLT
                     VTGVSRLVDAVTRIGEQQWS"
     gene            374..988
                     /locus_tag="PLO65_02430"
     CDS             374..988
                     /locus_tag="PLO65_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRD27137.1"
                     /translation="MLTQFDFIAGPLLLVSAIIGFTRGATKELMTVLAFLVAAILAVF
                     ALRFTAPAGKALIDPDWAGVGLSLLFVFGLSYIALRLLGATLERRIHETQTMGLLDRA
                     VGTGFGLVRAMVLLGAFSLLFHMATPAETAPRWVTGAMFYPLTEVSGRILKAFAPNGF
                     DVARVVAPRLEKAVRDSVTAPKAGEKGYEADERDAVDDLVEKTR"
     gene            985..2481
                     /gene="purF"
                     /locus_tag="PLO65_02435"
     CDS             985..2481
                     /gene="purF"
                     /locus_tag="PLO65_02435"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRD27138.1"
                     /translation="MTASRTSDRQVQPPARDPDDDSLRLECGVFGVFDVSDASAVVAL
                     GLHALQHRGQEACGIAAFDGQSFTTERHLGHVGEAFTGPDLVDRLPGQYAIGHTRYAT
                     AGGSGIRNVQPMFADLEAGGVALAHNGNLTNFLTLREQLVAEGAIFQSTSDSEVILHL
                     IARSRKARVVDRFIDAIGQIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLNGKPVLA
                     SETCALDMIGASFVRDVEHGEMVVISDTGVESLKPFQTGKARPCVFEYVYFARPDSVV
                     NGRSVYGVRKRMGMRLAEETPAGADVVVPVPDSGVPAALGYAQASGIPYELGIIRSHF
                     VGRTFIQPTQGVRELGVRMKHSPNRAVLEGKRVVLIDDSIVRGTTSRKIVRMVREAGA
                     TEVHLRSASPPIMWPDFYGIDMPDREKLLAARKSLAEMTAFLEVDSLGFLSVEGLYWA
                     LEAGPRDPACPQFTDHYFTGDYPTRLLDREIAEGGHDHGRQLSFLVSA"
     gene            2478..3458
                     /locus_tag="PLO65_02440"
     CDS             2478..3458
                     /locus_tag="PLO65_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRD27139.1"
                     /translation="MKQAGAGWLKGLRPDPYIIAILLMVVVASLLPVRGQGTVWFGWL
                     TKAAIALLFFLHGARLPRESVIAGLVHWRLHLAVLGSTFLLFPLLGLGVIQLPPSILP
                     TQLAAGVLYLCLLPSTVQSSIAFTSQARGNVAAAVVAASTSNLLGVVLTPLLVGLFLH
                     TQTGGLSVKAVQDVALQILLPFALGQLARPFVGGWVARHAKLVSYTDRGSILLVVYGA
                     FSAAVVGGLWRQVSGRELAVLLVICVLLLAVVLTATLMLARALKFATEDEIVTVFCGS
                     KKGLATGVPMASILFPAASVGFIVLPVMLFHQIQLMACAVIAQRYAARPA"
     gene            complement(3639..4016)
                     /locus_tag="PLO65_02445"
     CDS             complement(3639..4016)
                     /locus_tag="PLO65_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27140.1"
                     /translation="MPPISGTPIAYVYTTDRERALGFYRDTLGLPLQSSDGYGDYFQL
                     GDALMRLTVITDHLPSEHPVLGWQVEDCRATVQALAARGLKFNIYDGMGQDALGIWTS
                     PDGKSKIAFFNDPDGNALMLNEG"
     gene            4168..4920
                     /locus_tag="PLO65_02450"
     CDS             4168..4920
                     /locus_tag="PLO65_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD27141.1"
                     /translation="MESLPLKNRVALVTGATRGIGYQAALGLARAGAHVIALGRTQGG
                     LEALDDEIFAATGEHATLVPIDLKSPEGLDPLGAHIFERWGRLDIVVHAAAVLGGITP
                     TGHIDLKKWDEAVAVNLTATWRLIRSLEPLLRVSDAGRAIFLTTGRVVRPKAFWGAYA
                     ATKAGMEALVRCWADEMETTNVRAVILDPSTMRTRMRAEAYPGEDPATVTDPAEIAPL
                     IVQLSLADLGQPTSPVVFNDWKAAGGVLPANG"
     gene            complement(4957..6612)
                     /gene="der"
                     /locus_tag="PLO65_02455"
     CDS             complement(4957..6612)
                     /gene="der"
                     /locus_tag="PLO65_02455"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRD27142.1"
                     /translation="MSLKLAIVGRPNVGKSTLFNRLAGKKLAIVDDRPGVTRDRRFAS
                     GSLGDLDLTLIDTAGFEDVDDDSLEARMRAQTELAIQEADVVLFLIDAREGVTPGDEI
                     FAGILRRHQRPVILAANKAEGRAGDAGIAEAWSLGFGEPLAISGEHGEGMADLYALLS
                     GYAAEFGEDEEPDAADKPILIAIVGRPNAGKSTLVNRLIGEDRMLTGPEAGITRDAIS
                     VEWQWEGRNIRLVDTAGLRRKARVQEKLEKLSTSDSIRAITFAEVVLLVMDATHPFEI
                     QDLQIADLVEREGRALVFVLAKWDLVEDPQARLKAFQEHAERMLPQVKGAPVVALSAE
                     TGRGVERLMPALTKVYENWSTKVKTRDLNDWLAMAIQRHPPPAVGGKRIKPKYMAQTK
                     ARPPTFVLFSSRAEQMPEQYRRYLVNSMRESFDLPGVPIRLTLKSNRNPYAEGAEKGG
                     APSFQQGGLIQREKAKALAADAKAARAEAAAEEAGEARPKPAKKTVVRLTGVKAAKAR
                     AATALKFTKVKGAKGSRPGIQKLRGGGSVKGRPPRPTTRSGKK"
     gene            complement(6726..8096)
                     /locus_tag="PLO65_02460"
     CDS             complement(6726..8096)
                     /locus_tag="PLO65_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRD27143.1"
                     /translation="MISRNVGAAVLLAGAMALGGCSTVSAVGDAAGRLVPSFGKKDKP
                     QEVAGDGRRISIIAFDQRVEPADALKGADFFLPPALPQAEWPQPGGLGEQNVENVEAA
                     PTFEIAWRRDFGKGSDRTFHITTAPIVAGGRVYVMDAETDLAALDAQSGAIVWKASIH
                     PRARRDKDGFGGGIAFADGKLYVASGFRMVAQVDAATGKPGWKGKTEAPIHGAPVVTH
                     GRMFVVSTDNELLSFDTATGAPGWTFQALVEPARILASPSPAVSGETVVAAFASGEVV
                     ALRTANGNDLWNTTLSKASRTTALSEIRDVAGRPVIHMGDVYAASHGGVFAATDLRTG
                     QMRWSLPVASIDTPWPAGDVVYVISQAGEVICASRESGQVYWIKDLNAGVKKNKDRAL
                     FTGPVLASDRLVVVSSDGRAIALNPKTGEVLKTLKIGSAVLTSPVAANGMIYVVTDKG
                     ELVAIR"
     gene            complement(8093..8857)
                     /locus_tag="PLO65_02465"
     CDS             complement(8093..8857)
                     /locus_tag="PLO65_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD27144.1"
                     /translation="MVDVFEEVEDQIRTERYRSLAIKALPWALGAAAAALIAALAFWG
                     WDSYSKSQADKASETFMAALEASSQGDQTGAFAKFGEVAKSGARGYRGLALMAQGGLR
                     MEAGKTDEAVKLWDEAAKASDDPLLADLARLKSAFALLDTAPYTELEGRLTPLMEDKR
                     PYRLQAREALAFAKIMAGKATEARADFTYLSQVLGASEGQRKRAQAALALIDSGSAGA
                     IPAAARAAALLPPAPPQMPEGLMGPGGPQAQQGTPQ"
     gene            complement(8941..9528)
                     /locus_tag="PLO65_02470"
     CDS             complement(8941..9528)
                     /locus_tag="PLO65_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27145.1"
                     /translation="MAYEIRRLAPGLWPDLEALFGPRGATAGCWCQWVMLRKGETFDE
                     VKGETAHRRLEAQVRGGERRGLLAYADGGPVGWLAYGPRRGFPRIDRAPSLAAADADL
                     VWSVGCFFTRAGWRGKGVAGALLDGALSAIAADGGRTVEGYPVRLAAGERQAAAFVWT
                     GTTTLFEQHGFVQVDQRIKGKVRMRRHVGADHSTA"
     gene            complement(9541..10368)
                     /gene="panB"
                     /locus_tag="PLO65_02475"
     CDS             complement(9541..10368)
                     /gene="panB"
                     /locus_tag="PLO65_02475"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRD27146.1"
                     /translation="MSAHREEPVRRVAAPDIAARKGGEPIVCLTAYTAPVAGIFDEVC
                     DLLLVGDSVGMVVHGLPNTVGVTLEMMILHGQAVMRAARRAMVVVDMPFGSYEGSREE
                     AYANAARIMKETGAQAVKVESGPTVPDTIHYLVQRGIPVMGHVGLRPQAILVDGGFKA
                     KGRNEADRAGIIDEARATADAGAFAVVVEGVAEPLARDITAAIAPPTIGIGASAGCDG
                     QILVSDDMLGLFDWTPKFVRRYADLKGEIDKAARAYAGDVRARRFPAAAETYFAPKT"
     gene            complement(10385..10768)
                     /locus_tag="PLO65_02480"
     CDS             complement(10385..10768)
                     /locus_tag="PLO65_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27147.1"
                     /translation="MAELDFEMKLDRMFNEPPVMPDAEAFARLVEMRLDRGWNLRQVA
                     IGSAGLVAGLVGVSQLLGSRLLFDASEATQRNTAAFTSGVSGLVRTGENLSALPFGGE
                     ALWLAAALGVMALAFAVTRAVEEFQ"
     gene            complement(10761..>11034)
                     /locus_tag="PLO65_02485"
     CDS             complement(10761..>11034)
                     /locus_tag="PLO65_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRD27148.1"
                     /translation="AFVNAATRGSLSAAARIDLDEALKTLSPAERMCVSLCYGAGVSH
                     AEAAEALNAPLGTVKSHVKRGLDKLRLRLAPPEGANSEPAGSKGHG"
     assembly_gap    11035..11044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11045..11378)
                     /locus_tag="PLO65_02490"
     CDS             complement(<11045..11378)
                     /locus_tag="PLO65_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRD27149.1"
                     /translation="MAGARTPASLHDVELAALAAAGDRPAFGELVRRHGTAVRGLLRR
                     MGAEPSLADDIAQDAFLAAFERIAEFRGEGTFSAWVRKIAARLYVRRWRKESRFDAEA
                     EGEDESHGG"
     gene            complement(11411..11800)
                     /locus_tag="PLO65_02495"
     CDS             complement(11411..11800)
                     /locus_tag="PLO65_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HRD27150.1"
                     /translation="MDGSEVLVPLGFFLMLAAIFVGPAWLKSRERREMQATLRAAIEK
                     GQALPPEIIDAMTKNVKVAPTALSDLRAGVIWVAIGLGFAAFGFFIGFEAEEAFHPFL
                     GIAAIPAIIGLAYIALSFFNPNKAKQP"
BASE COUNT         1740 a         4218 c         4195 g         1931 t
ORIGIN      
        1 cggctggcca tggtgctggc cgtgctggag tcgcgctgcg gcctcgcctt cggcaacaag
       61 gatgtctacc tcaacgtcgc cggcggcctg cgcatcaccg agccggccgc ggacctcgcg
      121 gcggctgcgg ccctcgcctc ctcggcgctc gacgaggccc tgccgcagga ttgtgtggtg
      181 ttcggcgagg tcagcctgtc gggcgaggtt cggccggtgg gccgggcgga gacccgcctc
      241 aaggaagccg cgaagctggg gttcaggcgc gccctggcgc cgatcggcgc cggcgagagc
      301 ctgacggtga ccggggtgtc acggctggtc gacgccgtca cgcgcatcgg agagcagcaa
      361 tggtcatgat cccgtgctga cgcagttcga tttcatcgcc gggccgttgt tgctggtttc
      421 ggccatcatc ggcttcaccc ggggcgcgac caaggagctg atgacggttc tggccttcct
      481 tgtggcggcc atcctggcgg tgttcgccct gcggttcacg gcccccgcgg gcaaggccct
      541 gatcgacccg gactgggccg gggtcggcct gtcccttcta ttcgtctttg gtctgtccta
      601 tatcgcgctg cggctgcttg gcgcgacgct agagcggcgg atccacgaga cgcagaccat
      661 gggcttgctg gaccgggcgg tgggcacggg cttcggcctg gtcagggcca tggtcctgct
      721 gggcgccttc agcctgctgt tccacatggc cacgccggcg gaaacggcgc cgcgctgggt
      781 gaccggcgcg atgttctatc cgttgacgga ggtctccggg cggatcctca aggctttcgc
      841 gcccaatggc ttcgacgtgg ccagggtggt cgcgccgcgg ctcgagaagg cggtccgcga
      901 cagcgtgacc gcgcccaagg ccggcgaaaa ggggtatgag gcggatgagc gcgacgccgt
      961 cgacgatctg gtggagaaga cgcgatgacc gctagccgga ccagcgaccg acaggttcag
     1021 ccccccgccc gggatccgga cgacgacagc ctgcggctcg aatgcggcgt gttcggcgtg
     1081 ttcgacgtca gcgacgcctc ggccgtcgtg gccctgggcc tgcacgccct gcagcatcgc
     1141 ggacaggagg cctgcggcat cgccgccttc gacggccaga gcttcaccac cgagcggcat
     1201 ctgggccatg tcggcgaggc cttcaccggg ccggacctgg tcgatcgcct gcccggccag
     1261 tacgccatcg gccacacccg ctacgccacc gccggcggca gcggcatccg caacgtccag
     1321 ccgatgttcg ccgatctcga agccggcggc gtggccctcg cccataacgg caacctgacc
     1381 aatttcctga ccctgcgcga acagctggtc gccgagggcg cgatcttcca gtcgacctcg
     1441 gacagcgagg tcatccttca tctgatcgcc cgttcgcgga aggcgcgggt cgtcgaccgg
     1501 ttcatcgacg cgatcggtca gatcgagggc ggctacgccc tggtggcgct gaccaacaag
     1561 aaactgatcg gcgtgcgcga tccgctgggc atccgcccgc tggtgctggg cgacctgaac
     1621 ggcaagccgg tgctggcgtc ggagacctgc gccctggaca tgatcggcgc gagcttcgtg
     1681 cgcgacgtcg agcatggcga gatggtggtt atttccgaca ccggcgtgga gagcctcaag
     1741 ccgttccaga ccggcaaggc ccgcccctgc gtgttcgagt acgtctactt cgcccgtccc
     1801 gacagcgtgg tgaacggccg ctcggtctac ggcgtgcgca agcgcatggg catgcggctg
     1861 gccgaggaga cgccggccgg ggccgacgtg gtcgtgccgg tccccgacag cggcgtgccc
     1921 gcggcgctcg gctacgccca ggccagcggc attccctatg aactgggcat catccgcagc
     1981 cacttcgtcg gccgcacctt catccagccg acccagggcg tgcgcgaact gggagtgcgc
     2041 atgaagcaca gccccaaccg cgccgtgctt gagggcaagc gggtggtgct gatcgacgac
     2101 tcgatcgtgc gcggcacgac cagccgcaag atcgtgcgca tggtccgcga ggccggcgcc
     2161 accgaggtgc acctgcgctc ggccagcccg ccgatcatgt ggccggactt ctacggcatc
     2221 gacatgcccg atcgggaaaa gctgctcgcc gcccgcaagt cgctggccga aatgaccgcc
     2281 ttcctcgagg tcgacagcct cggcttcctg tcggtcgagg ggctctactg ggcgctcgag
     2341 gccggaccgc gcgacccggc ctgtccgcag ttcaccgacc actatttcac cggcgattac
     2401 cccacccgcc tgcttgaccg cgagatcgcc gagggtggcc atgaccacgg ccgccagctg
     2461 tcgttcctgg tgagcgcgtg aagcaggcgg gcgcgggctg gctgaaaggc ctcaggcccg
     2521 acccctacat catcgccatc ctgctgatgg tcgtggtcgc cagcctgctg ccggtgcgcg
     2581 gccaggggac ggtctggttc ggctggctga ccaaggcggc gatcgccctg ctgttcttcc
     2641 tgcatggcgc gcgtctgccg cgggagtcgg tcatcgcggg gctggtccac tggcgactgc
     2701 acctggcggt gctcggctcg accttcctgc tgttcccgct gctcgggctg ggcgtcatcc
     2761 agctgccgcc atcgatcctg ccgacccagc tggcggcggg cgtgctgtac ctctgcctgc
     2821 tgccctcgac ggtgcagtcg tcgatcgcct tcacctcgca ggcgcggggc aatgtcgcgg
     2881 cggcggtggt cgcggcttcg acatccaacc tgcttggcgt cgtgctgacg ccgctgctgg
     2941 tgggcctgtt cctgcacacc cagaccggcg gcctgtcggt caaggcggtg caggacgtgg
     3001 ccctgcagat cctgctgccg ttcgcgctgg gccagctggc ccggcccttc gtcggcggct
     3061 gggtggcgcg gcacgcgaag ctggtcagct acaccgaccg ggggtcgatc ctgctggtgg
     3121 tctacggcgc gttcagtgcg gccgtggtgg gagggctgtg gcgacaggtc agtggacggg
     3181 aactggccgt tctgctggtc atctgcgttc tgctgctggc ggtggtcctg accgcgaccc
     3241 tgatgctggc tcgggcgctg aaattcgcga ccgaggacga gatcgtcacc gtcttttgcg
     3301 gctcgaagaa ggggctcgcc accggcgtgc cgatggccag catccttttt ccggccgcca
     3361 gcgtcggctt catcgtcctg ccggtgatgc tgttccacca gatccagctg atggcctgcg
     3421 ccgtcatcgc ccaacgctac gcggcgcggc cggcctaggc tgaatcggca ttcaggggct
     3481 ggggctgtct ttttgccgat tgtccccgcg aaggcgggga cccaggcggt gtttcaaggc
     3541 ttgaagcgcc ggtgaagcat ccggaatggc ttgggtcccc gccttcgcgg ggacaatcgg
     3601 ggtttgatgg cctctcgatc gctgaatgtc gattggtcct aaccctcgtt cagcatcagc
     3661 gcgttgccgt cggggtcgtt gaagaaggcg atcttcgact tgccgtcagg tgaggtccag
     3721 atgcccaggg cgtcctgccc catgccgtcg tagatgttga atttcaggcc cctggcggcc
     3781 agggcctgga cggtcgcgcg gcagtcctcg acctgccagc ccagcaccgg gtgctccgaa
     3841 ggcaggtggt ccgtgatcac cgtcagcctc atcagggcgt cgccgagctg gaagtagtcg
     3901 ccgtagccgt ccgacgactg cagcggcagg ccgagcgtgt cgcgatagaa gcccagcgcc
     3961 cgctcacggt cggtggtgta gacataggcg atcggcgttc ctgagatcgg gggcatcgcg
     4021 ggggctcctg ttgatcgagc ctagcccggc gcaagcctcg gccgatagtg gggtcgtcgc
     4081 ccggcgaagc cgacgcaagc cgccgaaaat gcgcccgcgg ggacaggtgc gcttcgcgaa
     4141 ccgcccccct tcggctagaa gccccgcatg gaatcccttc ccctgaagaa ccgcgtcgcc
     4201 ctggtcaccg gcgcgacgcg cggcatcggc taccaggccg ccctggggct ggcccgcgcc
     4261 ggcgcccatg tcatcgccct gggccgcacc cagggcgggc tcgaggcgct cgacgacgag
     4321 atcttcgccg ccaccggcga gcacgccacc ctcgtgccca tcgacctcaa gtcgcccgag
     4381 gggctcgacc cgctcggcgc ccacatcttc gaacgctggg gcaggctcga catcgtggtc
     4441 cacgccgccg ccgtgctggg cggcatcact ccgacgggcc atatcgacct gaagaagtgg
     4501 gatgaggccg tcgccgtgaa cctgacggca acctggcgac tgatccgctc gctggagccg
     4561 ctcctgcggg tctccgacgc gggccgagcc atcttcctga ccaccggccg tgtcgtgcga
     4621 cccaaggcct tctggggggc ctacgcggcg accaaggccg gcatggaggc gctggtgcgc
     4681 tgctgggcgg acgaaatgga gacgaccaac gtccgcgccg tgatcctcga tccctcgacc
     4741 atgcgcacgc ggatgcgggc ggaggcctat ccgggcgaag accccgcgac cgtcaccgac
     4801 ccggccgaga tcgcgccgct gattgtccag ctgtccctgg ccgacctggg ccagccgacc
     4861 tcgccggtgg tgttcaacga ctggaaggcg gcgggcggcg tgctgccggc gaacgggtag
     4921 cgatgtagcg aaggggacag cttatcctgt ccccgactat ttcttccctg accgcgtcgt
     4981 cggccgcggc gggcgtccct tgaccgagcc gccgccgcgc agcttctgga tgccgggccg
     5041 cgagcccttg gcgcccttga ccttggtgaa cttcagggcg gtcgccgcgc gggccttggc
     5101 ggccttgacc ccggtcagcc tgaccacggt cttcttcgcc ggtttcggtc tggcctcgcc
     5161 cgcctcttcg gccgccgcct ccgcgcgcgc ggccttggcg tcggcggcga gcgccttggc
     5221 cttctcccgc tggatcagcc ccccctgctg gaaggacggt gcgccgccct tctcggcgcc
     5281 ctcggcatag gggttcctgt tgctcttgag cgtcagccgg atcggcacgc cgggcaggtc
     5341 gaaactctca cgcatcgagt tgaccagata gcggcgatac tgctcgggca tctgttcggc
     5401 gcggctggag aacagcacga acgtcggcgg ccgggccttg gtctgggcca tgtatttcgg
     5461 cttgatgcgc ttgccgccca cggcgggggg cgggtgacgc tggatggcca tggccagcca
     5521 gtcgttgagg tcacgcgtct tcaccttggt cgaccagttc tcatagacct tggtgagggc
     5581 cggcatcagc cgctcgacgc cgcggccggt ctcggcggag agggcgacca cgggcgcgcc
     5641 cttgacctgc ggcagcatgc gctcggcatg ttcctggaag gccttcagcc gcgcctgcgg
     5701 atcctcgacc agatcccact tggccagcac gaacaccagg gcccgccctt cccgctcgac
     5761 caggtcggcg atctgcaggt cctggatctc gaacgggtgg gtcgcgtcca tgaccagcag
     5821 caccacctcg gcgaaggtga tggcgcggat cgagtcagag gtggacagct tctccagctt
     5881 ctcctgcacc cgcgccttgc ggcgcaggcc ggcggtatcg accaggcgga tgttgcggcc
     5941 ttcccactgc cactcgaccg agatggcgtc gcgggtgatg ccggcctcgg gcccggtcag
     6001 catgcggtcc tcgccgatca ggcgattgac cagcgtcgac ttgccggcgt tgggacgacc
     6061 gacgatggcg atgaggatcg gcttgtcggc ggcgtcgggt tcctcgtcct cgccgaactc
     6121 ggcggcgtat cccgacagca gggcgtagag atcggccatg ccctcgccat gctcgccgga
     6181 gatggccagg ggctcgccga agcccagcga ccaggcctcg gcgatgccgg cgtcgccggc
     6241 gcgcccctcg gccttgttgg cggccaggat caccggccgc tgatggcggc gcaggatgcc
     6301 ggcgaagatc tcgtcgccgg gtgtcacgcc ctcccgggcg tcgatcagga acagcacgac
     6361 atcggcttcc tggatcgcca gctcggtctg ggcgcgcatg cgcgcctcca ggctgtcgtc
     6421 gtcgacatcc tcgaagccgg cggtgtcgat cagggtcagg tccaggtcgc ccagactgcc
     6481 gctggcgaag cgccggtcgc gggtcacgcc gggccggtcg tcaacgatgg cgagcttctt
     6541 gcccgccagc cggttgaaca gcgtggactt gccgacgttc ggcctgccga cgatggcgag
     6601 tttgagagac atgcggactt ctgaacgacg ttgaccgcgc tccggcgagg agcgcggtca
     6661 caggatagca gacaaggccc ggaaatcggg cgcggcctgc ggggccgcgc ccataacacg
     6721 aaaacctacc ggatcgccac cagttcgccc ttgtcggtca cgacatagat catgccgttc
     6781 gccgcgaccg gcgaggtcag cacggccgag ccgatcttca gggtcttgag cacttcgccc
     6841 gtcttcgggt tcagcgcgat ggcccggccg tcactcgaga cgaccaccag gcggtccgag
     6901 gccagcaccg gaccagtgaa cagggcccgg tccttgttct tcttgacccc ggcgttgagg
     6961 tccttgatcc agtagacctg accgctttcc cgcgaggcgc agatcacctc gccggcctgg
     7021 gagatgacgt agaccacgtc gcccgccggc cagggggtgt cgatcgaggc gaccggcagg
     7081 gaccagcgca tctggccggt gcgcaggtcg gtcgcggcga acaccccgcc gtggctggcg
     7141 gcgtagacgt cacccatatg gatcaccggc cggccggcca cgtcccggat ttccgacagg
     7201 gccgtggtgc ggctggcctt cgacagggtg gtgttccaca gatcgttgcc gttggcggtg
     7261 cgcagggcga ccacctcgcc cgaggcgaag gcggcgacga cggtttcgcc ggagaccgcc
     7321 gggctgggcg aggccaggat ccgggccggc tcgacaagcg cctggaaggt ccagccgggc
     7381 gccccggtcg ccgtgtcgaa gctcagcagc tcgttgtcgg tgctgacaac aaacatccgc
     7441 ccgtgggtga ccaccggggc gccgtggatc ggcgcctcgg tcttgccctt ccagccgggc
     7501 ttgccggtag cggcgtcgac ctgggcgacc atgcggaagc ccgaggcgac atagagcttg
     7561 ccgtcggcga aggcgatccc gccgccgaag ccgtccttgt cgcgccgcgc gcgcgggtgg
     7621 atcgacgcct tccagacaat cgcaccgctc tgggcgtcga gcgccgccag atcggtctcg
     7681 gcgtccatga catagacgcg gccgccggcc acaatcggcg ccgtggtgat gtggaaggtc
     7741 ctgtcggacc ccttgccgaa gtcgcgccgc caggcgatct cgaaagtcgg cgcggcctcg
     7801 acgttctcga cgttctgttc gccgagaccg cccggctgcg gccattcggc ctgcggcagg
     7861 gccggcggca ggaagaagtc ggcccccttg agggcgtcgg ccggctccac ccgctggtcg
     7921 aaggcgatga tcgagatgcg gcgaccgtcg ccggccacct cctgcggctt gtccttcttg
     7981 ccgaaggacg gcaccagcct gcccgccgca tcgccgacgg cgcttacggt ggagcagccg
     8041 ccaagcgcca tcgcgccggc gagaaggacg gcggcgccga cgttacgcga gatcattgag
     8101 gggttccctg ttgggcttgc gggccgccgg gtcccatcag accttccggc atttgcggcg
     8161 gcgccggcgg cagcagcgcg gcggcccggg ccgccgcggg aatggctccg gccgagccgg
     8221 aatcgatcag ggccagggcg gcctgggcgc gcttgcgctg cccttcgctc gcgcccagca
     8281 cctggctcag ataggtgaag tcggcgcggg cctccgtggc cttgccggcc atgatcttgg
     8341 cgaaggccag ggcctcgcgg gcctgcagcc ggtaggggcg cttgtcttcc atcagcgggg
     8401 tcagcctgcc ctcgagttcc gtgtagggag ctgtatcgag aagggcgaag gcggatttga
     8461 ggcgggccag atcggccagc agcggatcgt cggaggcctt ggcggcctcg tcccacagct
     8521 tgaccgcctc gtccgtcttg cccgcttcca tgcgcaggcc gccctgggcc atcagggcca
     8581 ggccgcgata gccgcgggcg ccggacttgg ccacctcgcc gaacttggcg aaggcgccgg
     8641 tctggtcgcc ctggctgctc gcctcgagag ccgccatgaa ggtctcggac gccttgtcag
     8701 cctgggattt cgaataggag tcccagcccc agaacgcgag cgccgcgatc aacgccgcgg
     8761 cggcggcgcc aagggcccac ggcaaggcct tgatcgccag gctgcgatac cgctccgtgc
     8821 gaatctggtc ttcgacttct tcaaaaacat cgaccacgaa cgggcgctcc gcgaaatccg
     8881 ctgattgagg ctgcggaacc tatagggtga gccgcgccgc tgcaacgcgg cgtgacgcgg
     8941 tcaggccgtc gaatgatcgg cgccgacatg ccgccgcatg cgcaccttgc ccttgatccg
     9001 ctggtcgacc tgcacgaagc catgctgctc gaacagcgtc gttgtcccgg tccagacgaa
     9061 ggcggcggcc tggcgctcgc cggccgccag cctgacggga tagccctcca ccgtgcgccc
     9121 gccgtccgcg gcgatcgcgg atagcgcgcc gtccagcagg gcgccggcca cgcccttgcc
     9181 ccgccagccg gccctggtga agaaacagcc gaccgaccag accagatcgg cgtccgcggc
     9241 cgccagggat ggagcacgat cgatgcgggg aaatccacgg cgtggtccat aggccagcca
     9301 gccgaccggg ccgccgtcgg cgtaggccag caggccgcgc cgctcaccac cacggacctg
     9361 ggcttccagc cgccgatgcg ccgtctcgcc cttgacctcg tcgaaggtct cgcccttgcg
     9421 cagcatgacc cactggcacc agcagccggc cgtcgcgcca cggggtccga acagcgcctc
     9481 caggtcgggc cagaggccag gcgccagccg ccggatctcg taagccaccg gcctgcgctc
     9541 ctaggtcttt ggcgcgaaat aggtttcggc cgcggccggg aagcggcgcg cgcggacgtc
     9601 cccggcatag gctcgggcgg ccttgtcgat ctcacccttc aggtccgcgt agcggcggac
     9661 gaacttcggc gtccagtcga acaggcccag catgtcgtcg ctgacgagga tctggccgtc
     9721 gcagccggcg ctggcgccga tgccgatggt cggtggggcg atggcggcgg tgatgtcgcg
     9781 ggcgagcggc tcggcgacgc cctctacgac caccgcgaac gccccggcgt cggccgtggc
     9841 gcgggcctcg tcgatgatcc ccgcccggtc cgcctcgttg cggcccttgg ccttgaaacc
     9901 gccgtcgacg aggatcgcct ggggccgcag cccgacatgg cccatgaccg gaatgccgcg
     9961 ctggaccaga taatggatcg tgtcgggcac ggtcgggccg ctttcgacct tgaccgcctg
    10021 ggcgcccgtt tccttcatga tccgggcggc gttggcgtag gcctcctccc gcgagccctc
    10081 gtatgagccg aagggcatat cgaccacgac catcgcccgg cgcgccgcgc gcatcaccgc
    10141 ctggccatgc aggatcatca tctccagcgt caccccgacg gtgttgggca ggccgtgcac
    10201 caccatgccc accgagtcgc cgaccagcag caggtcgcag acctcgtcga agattcccgc
    10261 gacgggggcg gtgtaggcgg tcaggcagac gatcggttcg ccgcccttgc gggcggcgat
    10321 gtccggagcg gcgacgcggc ggacgggctc ttcacgatgg gcggacagga aggcctcctg
    10381 tgggttactg gaactcctcc accgcccgtg tgacggcgaa cgccagcgcc ataacaccca
    10441 gagccgccgc cagccacagc gcctcgccgc cgaagggcag cgccgagagg ttttcaccgg
    10501 tccgcaccag gccgctgacg ccgctggtga aggccgccgt attgcgctgg gtcgcctcgc
    10561 tggcgtcaaa cagcaggcgc gagccgagca gctggctgac cccgaccagg cccgccacca
    10621 ggccggccga gccgatggcc acctggcgca ggttccagcc gcggtcgagg cgcatctcca
    10681 ccagccgggc gaacgcttcc gcgtccggca tgaccggcgg ctcattgaac atccggtcca
    10741 gcttcatctc gaagtcgagc tcagccatga cccttactcc ccgccggctc gctgttcgcg
    10801 ccttcaggag gcgcaagtct gagtcggagc ttatccagac cacgtttgac atgggatttg
    10861 accgttccca gaggcgcatt caaggcctcc gctgcttcgg cgtgtgacac gcccgctccg
    10921 taacagagcg atacgcacat ccgttccgcg ggcgagagcg tcttgagcgc ctcgtccaga
    10981 tcgatgcggg cggcggcgga aaggctgcct cgggtggcgg cgttgacgaa ggcgnnnnnn
    11041 nnnnccccgc cgtgactctc gtcctcaccc tccgcctcgg cgtcgaagcg ggactccttg
    11101 cgccaccggc gcacgtagag ccgcgccgcg atcttgcgga cccacgccga aaaggtgccc
    11161 tccccgcgga attccgcgat ccgctcgaag gcggcgagga aggcgtcctg ggcgatgtcg
    11221 tctgccagcg acggctcggc gcccatccgc cgcagcagtc cccgcaccgc cgtgccgtgg
    11281 cggcggacca gttcgccgaa ggcgggccgg tcgccggccg ccgcgagggc ggccagctcg
    11341 acatcgtgca ggctggcggg tgttcttgcc ccggccatga tcgtgcgctc ccctttcaat
    11401 cccgatcagg tcagggctgc ttggctttgt tcgggttgaa gaagctcagg gcgatgtagg
    11461 ccaggccgat gatcgcgggg atcgcggcga tgcccaggaa gggatggaag gcttcctcgg
    11521 cctcgaagcc gatgaagaag ccgaaggcgg cgaagcccag gccgatggcc acccagatca
    11581 cgccggcgcg caggtcgctc agcgcggtgg gggcgacctt cacgttcttg gtcatggcgt
    11641 cgatgatttc gggcggcagc gcctggccct tctcgatcgc ggcccgcagc gtcgcctgca
    11701 tttcgcggcg ctcgcggctc ttgagccagg ccggtccgac gaagatcgcc gccagcatca
    11761 gaaaaaagcc cagtggaacc agaacctcgg aaccgtccat gggaaatccc tcctcgatca
    11821 catggcgccg ccttgttgcg gtcgccctct gactagtaag aggcgcgggc cccggccaac
    11881 ggatgcgggc gaccggatga aatcttcgta acgcgcctca gcgaccggcg cggccaggtt
    11941 caagggtctg gccgaacgcc acgaaagcag ccgtcagcgt cagcaggacc gccgccggga
    12001 agaacgtgcg ggccaccggc tcgacgatcg cgctgatcgc cggagcgctg gcccagagcg
    12061 ccgccgccgc cgcggccgcg aacagcgcgc ctgt
//
LOCUS       DAORNX010000032        11918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4058_length_11918_cov_6.922954, whole genome shotgun sequence.
ACCESSION   DAORNX010000032 DAORNX010000000
VERSION     DAORNX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_11918_cov_6.922954"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLO65_02500"
     CDS             <1..190
                     /locus_tag="PLO65_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD27151.1"
                     /translation="LRFCGLPLAGVGLRSRTIYQAAIAHGASAAEWDPASTAAREAAG
                     LWRDIERRLGAGLAAERA"
     gene            280..1482
                     /locus_tag="PLO65_02505"
     CDS             280..1482
                     /locus_tag="PLO65_02505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRD27152.1"
                     /translation="MSIESQALRRIKPSATIGVSAKARALKAAGRDVIALSAGEPDFD
                     TPDNIKEAAIAAIRAGKTKYTDPDGMPELKAAICAKFARENGLTYSPGQVHVAPGGKP
                     VIFNALMATLNPGDEVVIPAPYWVSYPDMVLLAGGEPVAVETSPDNNFKLEPAALEAA
                     ITPRTRWLILNSPSNPTGGAYTRADLQALADVLLRHPQVWVLTDDMYEHLVFDDFEFT
                     TIAQVEPKLYDRTLTMNGVSKAYAMTGWRIGYAAGPEALIKAMGKIISQTTSNPSSIS
                     QWAAVEALNGPQDFIKPNAKLFQTRRDLVVSMLNQAQGIHCPTPEGAFYVYPSVAGLL
                     GKTAPSGKVIETDEDFASELLEAEGVAVVHGAAFGLSPYFRISYATSEAILEDACGRI
                     QRFCGNVK"
     gene            1487..1927
                     /locus_tag="PLO65_02510"
     CDS             1487..1927
                     /locus_tag="PLO65_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HRD27153.1"
                     /translation="MAVGPFNDDEWTVGEARAEDGVSMIVRFRSHLPSAAAREDWRHL
                     VQIGWSCESADDDGMPDDVENGRQDAFEDALQAQVESRGHGAQVASLTGGGVREWRYY
                     TRDPEVFFRSLNEALASHPEYPLEFTVFEDPEWDGLSELLPPRN"
     gene            complement(1974..>2755)
                     /locus_tag="PLO65_02515"
     CDS             complement(1974..>2755)
                     /locus_tag="PLO65_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRD27154.1"
                     /translation="MTRARWALATGGLVAAVCLGGLAAAQTIPRLGYLGEAAANTLEL
                     LGPAPAPGSPRAEADRAIFRATRAMKGSPRWAMALADFDERAVVSNMSCAAGVKMDLA
                     AMPATTRLIVHMAPDIIRAVEAPKRASARPRPYRLDPGETCAALPASEDNFDYPSGHS
                     AWGWAVGLVLAELLPERADSILVRARAVGEGRVICGVHNYSSIVAGQTVAAAIVAAEH
                     GKPEFQADLAAAKVELQAAAKAAPFTEGCDAEAKMLATPAW"
     assembly_gap    2756..2832
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2896..3981
                     /locus_tag="PLO65_02520"
     CDS             2896..3981
                     /locus_tag="PLO65_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRD27155.1"
                     /translation="MTDLFDPFEANGLRLRNRIALSPLTRTRADMAGKVGALQAEYYA
                     QRASAGLLITEATAVVPGGRGGAYCPGLWDDDQIPGWRQVTDAVHARGGLIFCQLWHA
                     GRLSHSSLSNGAGAPVAPSPMKQPGQMFIEGGFADYEEARALDIGELPGLVDRYRQAA
                     ARAKAAGFDGVELHAAHSYLIDAFLRDMTNRRTDAYGGAIENRARLLVEVLQALVGVW
                     GPERTAIRLAPIGHSYNAWDSDPEPLFTYIVEQLNALNIGWLDLVEGDTGVSRDCEPS
                     FDLQKLRRLFKGVTIANNLYDRDLALKARAGNTADMVAFGRAFIGNPDLVERLRLDAP
                     IVDAPRQSYYGGGARGYTDFPTLAEAS"
     gene            complement(3985..>4345)
                     /locus_tag="PLO65_02525"
     CDS             complement(3985..>4345)
                     /locus_tag="PLO65_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27156.1"
                     /translation="HVSADLIGAASRPGRGQKVTLEGRRKVRFRVDSVTLLPGGLARL
                     SSFGGYRGSTGDQSGTLEAIESLATARGSIVVSRDRFRVVAPSAGVATAMAPVIKACG
                     NLKMMIKRAEGREGELG"
     assembly_gap    4346..4364
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4538..5413)
                     /locus_tag="PLO65_02530"
     CDS             complement(4538..5413)
                     /locus_tag="PLO65_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27157.1"
                     /translation="MGRNDRLAVLGYRLGVITRSGISASTGQGTVSMQTTVELSGVSL
                     AQVRAIADAAYADLIGQLAATGRPMATLDEIRATKGYGLLTPAPTPFIKKPFADARTA
                     AIVSPNGQDLFFQHADTPLTDRSPMDLKNWRAINQMSVDLGNAVILTPVVVVDFAELS
                     GSGHKVYGGAASVGVKPGIYVVDFFSGLGANQAKIALAGDGGRALLQKRIAVGQAGEF
                     VKTAEYGNQAEVAWWNMMASTATSDVGRPTLSYEMSSYQYRVDPPLFANAVLDGCRAM
                     NRAYAEAAATLRPGR"
     gene            complement(5630..6661)
                     /gene="bla"
                     /locus_tag="PLO65_02535"
     CDS             complement(5630..6661)
                     /gene="bla"
                     /locus_tag="PLO65_02535"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HRD27158.1"
                     /translation="MAAVSAVPSPPWTPTADLLSAPLPAPPPGQQDLLRAEIERLGTS
                     LDGEVGIAVLDVTNGWSVNYNGHAHLPQQSVSKLWVAITVLNAIDRGDFSLDDTVVIH
                     KEDLSVFHQPLRARVGPDGYRTTIARLLAAEIQQSDNAANDALIRQVGGPWAVQVAIV
                     SKSLGEIRFGPGEKEMQTRAAGLTWKPEYSFGRAFWEDREKLPPEQRRAALEAYLANP
                     PDAASPLDIVDTLARLQRGELLSPESTAYLLNLMGGTQTGATRLRGGLEPGWRLQHKT
                     GTGQVMGSLATGNNDVGLLTAPDGRVYAVAVMIGRSRAPFEARQGLMQAVARAVIASH
                     RPAGPPPPT"
     gene            complement(6761..7003)
                     /locus_tag="PLO65_02540"
     CDS             complement(6761..7003)
                     /locus_tag="PLO65_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HRD27159.1"
                     /translation="MSDTPPDRLATNPRSPYYDAAVLERGVGVRFKGEEKTNVDEYCV
                     SEGWVRLSVGKTLDRHGNPMTVKLQGTVEPYFRDIA"
     gene            complement(7066..7686)
                     /locus_tag="PLO65_02545"
     CDS             complement(7066..7686)
                     /locus_tag="PLO65_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27160.1"
                     /translation="MYRLYWSPGAASLAVHWMLLELGVPFDLERADIDSGRNRSPDYL
                     QLNPQGRVPTLVVDGAPVAETAALLMLLAERHPRAGLAPALDSPDRAPWLEWMLYLAN
                     TLQPAFRLWFYPDDLPGADREAIRAAIEAGFDRIDARLAGRRFMIGEALTTVDLLATM
                     LMRWSRNMPRPATAWPNIAGYLAAVRARPALREVHAREGLTDWIDG"
     gene            complement(7754..7990)
                     /locus_tag="PLO65_02550"
     CDS             complement(7754..7990)
                     /locus_tag="PLO65_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HRD27161.1"
                     /translation="MIDASLIREHLEVVGSDDKHVGKVDKVMGTDIELAKFDLGAGFK
                     HHLIPTSWVDFVDDEKVHLTLTADAAKAAWREKH"
     gene            8116..8994
                     /locus_tag="PLO65_02555"
     CDS             8116..8994
                     /locus_tag="PLO65_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRD27162.1"
                     /translation="MKALLVAALIALLPAAALARPGLDASIGPDGVTITDSERPVLFY
                     RTKPADPAVEPGRLNYIHPLYAPDGTILTEDRPAVLPEQRGLFWAWRQVRLNGQTVAD
                     GWAMKGLTLFVRQTNFDGERGGVGVLTLQVDWIVNSGPELQFVAREVTKLRIQPLKAG
                     ARRIDIETSLTPLVDGLSLGGSDDAKGYGGVALRLVSPEKLVFGSDGRAVAPADTPVT
                     AGSSMGFSWPGQPGLAKWAVGLACKAEGRPISLWILRRGPSMQNCVWPGRVPVALAKG
                     KPLRLQSTVIVRPAGR"
     gene            8991..9167
                     /locus_tag="PLO65_02560"
     CDS             8991..9167
                     /locus_tag="PLO65_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HRD27163.1"
                     /translation="MTEPINLNRFRKARARSQARATAAENRVRFGRSRADQTRMEAER
                     EKAARDLDGSKREP"
     gene            9176..9415
                     /locus_tag="PLO65_02565"
     CDS             9176..9415
                     /locus_tag="PLO65_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRD27164.1"
                     /translation="MRKRSVNLHGHATSLALEPEFWAVLDAAAKADGLSLAGLIVRID
                     DARGERPLASACRVFALEQSSLLPTLVGSCQAKPD"
     gene            complement(9493..10278)
                     /locus_tag="PLO65_02570"
     CDS             complement(9493..10278)
                     /locus_tag="PLO65_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HRD27165.1"
                     /translation="MAFDSAPAATPPAPPARDRNAFRLQPLRAARALGKLIKDKEDTA
                     QVFEIMRALSGNTVVKGYRRLLSTPQGGRIAYEREEFCERLSDKAWLNSFGPGTVGEA
                     YRNFIAPRGLSAEGLAEESRKTADGEIDAPHPFAWFGRRMRDVHDVWHVLTGYGTDGL
                     GEACVVAFSYSQTKSLGFALIAHAGAHQYEKIGNGHPYRKAVMQAFRHGRAAAWLPQV
                     DYVRLFGENLEAARARLGIKPPTVYQSIPADQRDRPLSRRLDA"
     gene            complement(10559..10777)
                     /locus_tag="PLO65_02575"
     CDS             complement(10559..10777)
                     /locus_tag="PLO65_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27166.1"
                     /translation="MARRTGRRGVTGFILGVMFILLAIAAWTVWNNRLDVTRPAALEL
                     TLPQAPELPDPTPMPNPQPAPAPVPTPG"
     gene            complement(10782..10988)
                     /locus_tag="PLO65_02580"
     CDS             complement(10782..10988)
                     /locus_tag="PLO65_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27167.1"
                     /translation="MAEQSSSGGATPWLAFIVGGLLVVVAIVAFMMYSGQSPVPTKSV
                     DVNIEAPKMPDAPKLPDAPAPTPG"
     gene            11086..>11918
                     /locus_tag="PLO65_02585"
     CDS             11086..>11918
                     /locus_tag="PLO65_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRD27168.1"
                     /translation="MSDSPLPQPRISDLARVDGAPAYLDGLNPEQREAVEATEGPVLV
                     LAGAGTGKTRVLTTRLAHILSAGKARPWELLSVTFTNKAAREMRERITQIIGPQAEGL
                     RWLGTFHSVAAQILRRHAELVGLKSSYTILDDDDQERLIKQILLAENIDAKRWTPKTL
                     SGLIDHWKNRGWTPDRLPPSEGAVFANNRGQQLYRLYQERLRVLNACDFGDLLLHNLT
                     IFQQHPDVLAEYHDRFRYILVDEYQDTNVAQYLWLRLLAQKRQNVCCVGDDDQSIYGW
                     RG"
BASE COUNT         1775 a         4163 c         4104 g         1780 t
ORIGIN      
        1 cctgcgcttc tgcggcctgc cgctggccgg cgtcggcctg cgctcacgga ccatctacca
       61 ggcggccatc gcccatggcg cgtccgccgc cgaatgggac ccggcctcga ccgccgcccg
      121 cgaggcggcc ggcctgtggc gcgacatcga gcgtcgcctc ggagcgggtc tggccgccga
      181 gcgagcctga tcgaccgccg cttcgttgcg ccgcagcacg ggcgcgtcta tgaccccggg
      241 caatcctgac aatccgagtc cggggagccc tgtacagcca tgtcgatcga gtcccaggcc
      301 ctgcgccgca tcaagccgtc cgcgaccatc ggggtcagcg ccaaggcccg cgccctgaag
      361 gccgccggcc gcgacgtgat cgccctgtcg gccggagagc cggacttcga cacccccgac
      421 aacatcaagg aagcggccat cgccgccatc cgggcgggca agaccaagta caccgacccg
      481 gacggcatgc ccgagctgaa ggcggccatc tgcgccaagt tcgcgcgcga gaacggcctg
      541 acctacagcc cgggccaggt gcatgtggcg ccgggcggca agccggtgat cttcaacgcc
      601 ctgatggcga ccctgaaccc cggcgacgag gtggtcatcc cggcgcccta ctgggtgtcc
      661 tatccggaca tggtgctgct ggccggcggc gagccggtgg cggtggaaac cagccccgac
      721 aacaacttca agctggaacc ggcggcgctg gaggcggcga tcacgccgcg cacccgctgg
      781 ctgatcctca acagcccgtc caacccgacc ggcggtgcct acacccgcgc cgacctgcag
      841 gcgctggccg atgtgctgct gcgccatccg caggtctggg tgctgaccga cgacatgtac
      901 gagcacctgg tgttcgacga cttcgagttc acgaccatcg cccaggtcga gccgaagctc
      961 tacgaccgca ccctgacgat gaacggtgtt tccaaggcct acgccatgac cggctggcgg
     1021 atcggctacg cggccggccc cgaggcgctg atcaaggcca tgggcaagat catcagccag
     1081 accacctcca acccctcgtc gatcagccag tgggcggcgg tcgaggcgct gaacggtccg
     1141 caggacttca tcaagccgaa cgcgaagctg ttccagaccc gccgcgacct ggtcgtgtcg
     1201 atgctcaacc aggcccaggg catccactgc ccgacgccgg aaggcgcctt ctacgtctat
     1261 ccgtcggtgg ccggcctgct cggcaagacc gcgcccagcg gcaaggtcat cgaaaccgac
     1321 gaggacttcg cctccgaact gctggaagcc gaaggcgtgg ccgtggtcca cggggcggcc
     1381 ttcggcctgt cgccctattt ccgcatcagc tacgcgacca gcgaggcgat cctcgaggac
     1441 gcctgcggcc ggatccagcg cttctgcggc aacgtgaagt agggccatgg cggtcggccc
     1501 gttcaacgac gacgaatgga cggtgggcga ggcccgggcc gaggacggcg tgtcgatgat
     1561 cgtccggttc cgctcacacc tgccctccgc ggcggcgcga gaggactggc gccacctggt
     1621 gcagatcggc tggtcctgcg agtcggcgga cgacgacggc atgcccgatg acgtcgagaa
     1681 cggccggcag gacgcgttcg aggacgccct gcaggcgcag gtcgagagtc ggggtcacgg
     1741 cgcccaggtc gccagcctga ccggcggcgg cgttcgcgag tggcgctact acacgcgcga
     1801 tcccgaggtc ttcttccggt cgctgaacga ggcgctcgcc agccaccccg aatacccgct
     1861 ggaattcacg gtgttcgagg atcccgagtg ggacggtctg tccgagctgc tgccgccgag
     1921 gaactgacaa ggaagcattc gggggacatg cgcttcaatg cgtgtccccc tggtcaccag
     1981 gccggggtcg ccagcatctt cgcctcggcg tcgcagccct cggtgaaggg cgcggccttc
     2041 gcggccgcct gaagctcgac cttcgccgcg gcgagatcgg cctggaactc gggcttgccg
     2101 tgttcggcgg cgacaatggc ggcggccacg gtctggccgg ccacgatcga actgtagttg
     2161 tgcactccgc agatgacccg cccctcgccc accgcccggg cgcggacgag gatggagtcg
     2221 gcccgctccg gaagcagctc ggccagcacc aggcccacgg cccagcccca ggccgaatgg
     2281 ccggacggat agtcgaaatt gtcctcgctg gccggaaggg cggcgcaggt ctcccccgga
     2341 tcgagccggt agggccgcgg tcgggcggac gcccgcttcg gggcctcgac cgcccggatg
     2401 atgtccggcg ccatgtgcac gatcaggcgg gtggtggcgg gcatggccgc cagatccatc
     2461 ttcacgccgg cggcgcaact catgttgctg accacggccc gctcgtcgaa gtcggccagg
     2521 gccatggccc agcggggcga gcccttcatg gcgcgggtcg cgcggaagat cgcccggtcg
     2581 gcctcggcgc gtggcgatcc gggcgcgggc gccgggccca gcaattcgag ggtgttggcg
     2641 gcggcctcgc ccaggtagcc gaggcgggga atcgtctggg ccgccgccag gccgccgagg
     2701 cagaccgctg cgaccagccc gccggtcgcc aaagcccaac gggccctcgt catggnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnccccttgc gcctatcccg acggaacgcg gcaggcttgc cgcctggact
     2881 tcgcggagcc gcaccatgac cgacctgttc gaccccttcg aggccaacgg cctgcgcctg
     2941 cgcaaccgca tcgcgctgtc gccgctgacg cggacgcggg ccgacatggc cggcaaggtc
     3001 ggggcgctgc aggccgagta ctacgcccag cgcgcctcgg ccggcctcct gatcacggag
     3061 gcgaccgctg tggtccccgg cggtcgcggc ggcgcctact gccccggcct gtgggacgac
     3121 gaccagatcc cgggctggcg gcaggtcacc gacgcggttc acgcaagggg cggcctgatc
     3181 ttctgccagt tgtggcacgc cggccgtctg agccactcgt cgctgtcgaa cggcgcgggc
     3241 gcgccggtcg ccccgagccc gatgaaacaa cccggccaga tgttcatcga aggcggcttc
     3301 gccgactacg aggaggcccg cgcgctcgac atcggcgaac tgcccggcct ggtcgaccgc
     3361 taccgccagg ccgccgcgcg ggccaaggcc gcgggcttcg acggcgtcga gctgcatgcc
     3421 gcccacagct acctgatcga cgccttcctg cgcgacatga ccaatcgccg gaccgacgcc
     3481 tacggcggcg cgatcgagaa tcgcgcgcgc ctgctggtcg aggtgctgca ggccctcgtc
     3541 ggggtctggg gtccggaacg gacggccatc cgcctggccc ccatcgggca ctcctacaat
     3601 gcctgggaca gcgatcccga gccgctgttc acctacatcg tcgaacagct gaacgccctg
     3661 aacatcggct ggctggacct ggtcgagggc gacacgggcg tctcgcgcga ctgcgaaccg
     3721 tcgttcgacc tgcagaagct ccgccggctg ttcaagggcg tgaccatcgc caacaacctc
     3781 tatgaccgcg acctggcgct gaaggcccgg gccgggaaca cggccgacat ggtcgccttc
     3841 ggccgggcct ttatcggcaa tccggacttg gtcgagcggc tgcggctgga cgctccgatc
     3901 gtcgatgcgc cgcggcagag ctactacggc ggcggcgcac ggggttacac cgacttcccg
     3961 accctggccg aggcaagctg acccttatcc cagctcgcct tcacggcctt cagcccgctt
     4021 gatcatcatc ttcagattgc cgcaggcctt gatcaccggc gccatggccg tggcgacgcc
     4081 cgccgagggg gcgacgaccc ggaagcggtc gcggctgacg acgatcgacc ccctggccgt
     4141 ggccagggac tcgatggcct caagcgtgcc ggactggtcg cccgtgctgc cgcgatagcc
     4201 gccgaaactg gacaggcgcg ccaggccgcc cggcaggagg gtaacactgt cgacgcggaa
     4261 cctgaccttg cgccgccctt caagcgtgac cttctggccg cggcccgggc gcgaggctgc
     4321 gcctatcagg tcggcactga catgcnnnnn nnnnnnnnnn nnnncagcag cccgtcggcc
     4381 gcgcaggtga cggtcaggct ggtcccgttt ccgagaccga tggccgccac gcgattccgg
     4441 ccggccgatt gccggatcca gaccggcgcg cgcgcctccg ccggcggggc cgaaagcagc
     4501 acgccggcga ggatggcgac caccggccgg ctgaccgtca tcggccgggc cgcagcgtcg
     4561 cggccgcttc ggcataggcc cggttcatgg cccggcagcc gtcaaggacg gcgttggcga
     4621 acaggggcgg gtcgacccga tactggtagc tggacatttc gtaggacagg gtcggccggc
     4681 ccacgtcgga ggtggcggtg ctggccatca tgttccacca ggcgacctcc gcctggttgc
     4741 cgtactccgc ggtcttcacg aactcccccg cctggcccac cgcgatcctc ttctgcagca
     4801 gagccctgcc cccatcgcct gcgagagcga tcttggcctg gttggcgccc agaccgctga
     4861 agaaatcgac cacatagatg ccgggcttca cgccgacgct cgcggcgccc ccgtagacct
     4921 tgtggcccga tccgctgagc tcggcgaaat cgacgaccac gaccggtgtc agaatgaccg
     4981 cgttgccgag gtctacggac atctggttga tggcgcgcca gttcttcagg tccatcggcg
     5041 acctgtccgt gagcggcgtg tcggcgtgct ggaagaacag gtcctgaccg ttgggcgaga
     5101 cgatcgccgc cgtgcgggcg tccgcgaagg gctttttgat gaacggcgtc ggcgccgggg
     5161 tgagcaggcc gtagcccttg gtggcgcgga tttcgtcgag ggtcgccatg gggcggccgg
     5221 tggccgccaa ctgcccgatc aggtccgcgt aggccgcgtc cgcgatcgcg cggacctggg
     5281 cgagagagac gccgctcagt tccacggtcg tctgcatgct caccgttccc tggccggtcg
     5341 aggcgctgat gccgctgcgg gtgatgacgc ccagccggta gccgagcacc gccagacgat
     5401 cgtttcgtcc catggctgtc ttgatcgccc gactatcgag cacggacagt gaaacctgat
     5461 ccggcgagac gtaagtctgg gccacggcgg gcaaagcggc gccggcggcg agcgcggccc
     5521 ctccgagaac gacatcgcga cgtgaaacca tcggcccttc cccttctcaa acgggagaga
     5581 gggcaggatt cagccgtcat gcgccggcca catgccacat ttggggggct caggtcggcg
     5641 gcgggggtcc cgccggccga tggctggcga tcaccgccct ggccacggcc tgcatcaggc
     5701 cctgccgcgc ctcgaacggg gcccgcgacc ggccgatcat caccgccacc gcatagaccc
     5761 gcccgtcggg ggcggtcagc aggccgacgt cgttgttgcc ggtggccagc gaccccatca
     5821 cctggccggt gccggtcttg tgctgcagcc gccagccggg ttccagcccg ccgcgcagcc
     5881 gcgtggcgcc cgtttgcgtg ccgcccatca ggttcagcag ataggccgtc gactccggcg
     5941 acagcagttc gccccgctgc agccttgcca gggtgtcgac gatgtccagc gggctggccg
     6001 cgtcgggcgg gttggccaga taggcttcca gcgccgcgcg gcgctgctcc ggcggcagct
     6061 tctcgcggtc ctcccagaag gcccggccga agctgtattc cggcttccac gtcaggcccg
     6121 ccgcgcgggt ctgcatctcc ttctcgcccg gcccgaagcg gatctcgccc aggctcttgc
     6181 tgacgatggc cacctgcacg gcccaggggc cgccgacctg gcggatcagg gcgtcattgg
     6241 cggcgttgtc gctctgctgg atctcggcgg ccagcagccg ggcgatggtg gtccggtagc
     6301 cgtccggtcc gacccgggcg cgcagcggct ggtggaagac gctgagatcc tccttgtgga
     6361 tcaccacggt gtcgtcgagg gaaaagtcgc cccggtcgat cgcgttgagc acggtgatcg
     6421 ccacccacag cttgctgacg ctctgctgcg gcagatgtgc gtggccgttg tagttgaccg
     6481 accagccgtt ggtgacgtcg agcaccgcga tgccgacctc gccgtcgagg ctcgtcccga
     6541 gccgttcgat ctcggcccgc agaaggtcct gctggcccgg cggcggtgca ggcaggggcg
     6601 cggacagcag gtcggccgtc ggagtccagg gcggcgacgg cacggcggac acggcggcca
     6661 tggccacgcc gccgatcaga agtccgccga taagggatcg tccccgtcgc atggccgcat
     6721 ttgaccattg tcggcgacgt caggccaccg ttgtcggccg tcaggcgata tcgcggaaat
     6781 agggctcgac ggtcccctgc agcttgacgg tcatcgggtt gccgtggcga tcgagggtct
     6841 tgccgacgct cagccgcacc cagccctcgc tgacgcagta ttcgtcgacg ttggtctttt
     6901 cctcgccctt gaagcggacg cccacgccgc gctcaaggac ggcggcgtcg taataggggc
     6961 tgcgcgggtt ggtcgcgagg cggtcggggg gcgtgtcgct catgtcggtc ggctctcagg
     7021 cgtcaaggcg cgggtgatag ccacagtcgc gcgcgaagcc aaccgtcagc cgtcgatcca
     7081 gtcggtcagg ccttcgcgcg cgtggacctc acgcagggcc ggacgggccc gcacggccgc
     7141 caggtagccg gcgatgttcg gccaggccgt cgccggcctt ggcatgttgc gcgaccagcg
     7201 catcagcatc gtggccagca gatcgacggt cgtcagcgcc tcaccgatca tgaaccgccg
     7261 gcccgccagc cgggcgtcga tgcggtcgaa gccggcctcg atggccgcgc ggatcgcctc
     7321 gcggtcggcg cccggcaggt cgtcgggata gaaccacagc cggaaggccg gctgcagggt
     7381 gttggccagg tagagcatcc attccagcca aggggcgcgg tcgggcgagt cgagggccgg
     7441 ggccagcccg gccctgggat gccgctcggc cagcagcatc aggagggcgg cggtctcggc
     7501 gacgggcgcg ccgtcgacga ccagggtcgg cacccgtccc tgggggttca gttgcagata
     7561 gtcgggcgag cgattccggc cgctgtcgat gtcggcccgc tccaggtcga acgggacgcc
     7621 aagctcgagc agcatccagt gaaccgcgag gctggccgcg ccgggcgacc aatagagcct
     7681 gtacatgcga aaacccctcg cctgtgacga cgaggggcca tttccgcgaa aagccgtggg
     7741 ctgtcgagag gactcagtgc ttctcgcgcc aggcggcctt ggccgcgtcg gcggtcaggg
     7801 tcaggtgcac cttctcgtca tcgacgaaat cgacccacga ggtcgggatc aggtggtgct
     7861 tgaagccggc gccgaggtcg aacttggcca gctcgatgtc ggttcccatc accttgtcga
     7921 ccttgccgac gtgcttgtcg tccgagccga cgacttcgag gtgttcgcgg atgagcgacg
     7981 cgtcaatcat ggtcatgtct ccggtcattt gaagggccgc cccgcctcgc caactaacgg
     8041 gttcagagct caaccggttg cgcatcgcgc gccgttcttg ttcagtcccg tcccacgcca
     8101 gttgaaggtt ccggcatgaa ggctctcctc gtcgcggccc tgatcgcgct gctgccggcc
     8161 gccgccctcg cccgccccgg gctcgacgcc agcatcggcc ctgacggggt gaccatcacc
     8221 gacagcgaac ggccggtgct gttctatcgc accaagccgg ccgacccggc ggtcgagccc
     8281 ggccggctca attacatcca cccgctctac gcgccggacg gcacgatcct caccgaggac
     8341 cggccggcgg tcctgcccga gcagcgcggc ctgttctggg cctggcggca ggtccgcctg
     8401 aacggccaga cggtcgctga cggctgggcc atgaagggcc tgaccctgtt cgtgcgccag
     8461 accaatttcg acggcgagcg cggcggcgtg ggcgtgctga ccctgcaggt cgactggatc
     8521 gtcaacagcg ggcccgaact gcagttcgtc gcgcgcgagg tgacgaagtt gcggatccag
     8581 ccgctgaagg ccggcgcgcg gcgaatcgac atcgagacca gcctgacccc tctggtcgac
     8641 ggcctgtcgc tcggggggag cgatgacgcc aagggctatg gcggcgtcgc gctgcgactg
     8701 gtcagccccg aaaagctggt gttcggctcg gatggccgcg ccgtcgcgcc ggccgacacg
     8761 ccggtcacgg ccggctcttc gatgggcttc tcctggcccg gtcagcccgg cctggcgaaa
     8821 tgggccgtgg gcctggcctg caaggccgag ggccgaccga tcagcctgtg gatcctgcgc
     8881 cgcggcccgt ccatgcagaa ctgcgtctgg cccggccgcg tcccggtcgc cctggccaaa
     8941 ggcaagcccc tgcgcctgca gtcgacggtc atcgtccggc cggccggccg atgaccgagc
     9001 cgatcaacct caaccgcttc cgcaaggcca gggccaggag ccaggccagg gccacggcgg
     9061 ccgagaaccg cgtccgcttc ggccgctcca gggccgacca gacgcggatg gaggccgagc
     9121 gtgagaaggc cgcgcgcgat ctcgacggca gcaagcgcga accgtgaatc cgaccctgcg
     9181 caagcgttcg gtcaacctgc atggccacgc cacgtcgctg gccctggagc cggagttctg
     9241 ggccgtgctc gacgcggcgg cgaaggcgga cggcctcagc ctggccggcc tgatcgtccg
     9301 catcgacgac gcgcggggag agcggccgtt ggccagcgcc tgccgggtgt tcgcgctcga
     9361 acaatcatcc ttgctcccca cccttgtggg gagctgtcag gcgaagcctg actgagggga
     9421 gtcggtgcac gcccccccct ccaccgcctc cggcggtccc cctccccagc ggagctgggg
     9481 aggattattt ctctaagcgt ccagcctgcg gctgagcggc cggtcgcgct ggtcggcggg
     9541 gatgctctgg tagaccgtcg gcggcttgat ccccagtcgc gcgcgggcgg cctcgagatt
     9601 ctcgccgaac agcctgacgt agtcgacctg tggcagccag gcggcggccc tgccatggcg
     9661 gaacgcctgc atgaccgcct tgcggtaggg atggccgttg ccgatcttct catactggtg
     9721 tgcgcccgcg tgagcgatca gggcgaagcc gaggctcttg gtctggctgt aggagaaggc
     9781 caccacgcag gcctcgccca ggccgtcggt gccgtagccg gtcagcacat gccagacatc
     9841 gtgcacgtcg cgcatgcgtc ggccgaacca ggcgaacgga tgcggcgcgt cgatctcgcc
     9901 gtcggcggtc ttgcggcttt cctcggccag gccctcggcg ctcaggcccc gcggggcgat
     9961 gaagttgcga taggcctcgc ccaccgtgcc ggggccgaag ctgttcagcc acgccttgtc
    10021 cgacagccgc tcgcagaact cctcgcgctc gtaggcgatg cggccgccct gcggcgtcga
    10081 gagcaggcgg cgatagccct tgaccaccgt gttgccggac agcgcccgca tgatctcgaa
    10141 cacctgggcg gtgtcttcct tgtccttgat cagcttgccg agcgcacgcg ccgcccgcag
    10201 cggctgaagg cggaaggcgt tgcggtcgcg cgccggcgga gcgggcggcg tcgcggcggg
    10261 ggcggagtcg aaggccattg gcggcatcct gtgtcttgcg actccgaaag tgacgacccc
    10321 gtcatctttt tgcaaggggt cgcgtaacgg cggcctccga ctgttgcaaa ccggtcgcag
    10381 aattggctgt cccgcctgcc gttgttactc cgaccgaagc gcgtggcgcg aagcgccggg
    10441 gcccggattt ggcctgaggc gccctggggt cgttgtctcg ccgattgttc ccgcgaaggc
    10501 gcggaccaat cggagccttt gacagcgcct ggcgctgaat ggccatcggc cctcgcgact
    10561 accccggagt cggcacgggc gcaggcgccg gctgcgggtt gggcatcggg gtcgggtccg
    10621 gcagctcggg agcctggggc agggtcagct cgagcgccgc cgggcgcgtg acgtcgaggc
    10681 gattgttcca gacggtccag gcggcgatcg cgagcaggat gaacataacc cccaggatga
    10741 agccggtgac gccccgtcgg cctgtccgcc tggccatggc gtcagcccgg cgtcggcgcc
    10801 ggcgcatccg gcagcttcgg cgcgtcgggc atcttcggcg cctcgatgtt gacgtcgacg
    10861 gatttggtcg ggaccggcga ctggcccgag tacatcatga aggcgacgat cgccacgacg
    10921 accagcaggc cgccgacgat gaaagccagc cagggcgtgg cgccgccgct tgaggattgt
    10981 tcggccatgg gggtttcctt ccctgaaaca ctgtgaggcg attaacgacg gcccgcccgg
    11041 ccggttccgc ttaggcgccg cgcaccatgt gctacatatg ttccgatgtc cgactctccc
    11101 ctgccccagc cccgcatcag cgacctggcc cgcgtcgacg gcgcgccggc ctatctcgat
    11161 ggcctgaatc ccgaacagcg ggaggcggtc gaggcgaccg aaggcccggt gctggtgctg
    11221 gccggcgcgg gcacgggcaa gacccgtgtg ttgaccaccc gcctggcgca catcctgtcc
    11281 gccggcaagg cgcggccatg ggagctgctc agcgtcacct tcaccaacaa ggccgcgcgc
    11341 gagatgcgcg agcggatcac ccagatcatc gggccccagg ccgagggcct gcgctggctg
    11401 ggcaccttcc actcggtggc cgcccagatt ctgcgccgcc acgccgagct ggtcggcttg
    11461 aagtcgagct acaccatcct cgacgacgac gaccaggagc ggctgatcaa gcagatcctg
    11521 ctggccgaga acatcgacgc caagcgctgg acgcccaaga ccctgtcggg gctgatcgac
    11581 cactggaaga accgcggctg gacgccggac cgcctgccgc cctcggaggg cgcggtgttc
    11641 gccaacaacc gcggccagca gctgtaccgc ctgtaccagg agcgcctgcg ggtgctgaac
    11701 gcctgcgact tcggcgacct gttgctgcac aacctgacga tattccagca acatcctgat
    11761 gtgcttgcgg aatatcatga ccgcttccgc tacatcctgg tcgatgaata ccaggacacc
    11821 aacgtcgccc agtacctctg gctgcggctg ctggcgcaga agcggcagaa tgtctgctgc
    11881 gtcggcgacg acgaccagtc gatctacggc tggcgcgg
//
LOCUS       DAORNX010000033        11913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4059_length_11913_cov_8.489712, whole genome shotgun sequence.
ACCESSION   DAORNX010000033 DAORNX010000000
VERSION     DAORNX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_11913_cov_8.489712"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLO65_02590"
     CDS             <1..525
                     /locus_tag="PLO65_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27169.1"
                     /translation="AAERPDAARRLVLFDPVVMTAERVAAVGPDGLKASPLAVGARRR
                     RATFPDREAAFQAYSGRGAFRTWSDADLRGYLADGLLEGDDGQVHLSCRPDWEAANFA
                     AHHHDTIGAFARVVAPIRILKAEIGSTCGLEAHEGEVLAGGDVRMETVPGTSHFLPME
                     RPDLVRSALMAAAG"
     gene            complement(644..1564)
                     /locus_tag="PLO65_02595"
     CDS             complement(644..1564)
                     /locus_tag="PLO65_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD27170.1"
                     /translation="MEIWRGGVNTWECDEMGHMNVRFYVTKAIEGLVGLAAALGLPHA
                     FSAHAGSTLMVREQHIRFLREAHAGAALHMTGGVLEMGEDEARLLMLLWHSDSGALAA
                     SFQTVVAHVTPAEGRPFPWSRRTLDQAAALTIGLPDQAMARSISLAPTASIAGLARAE
                     ELGLVRIAQGAVSPADCDLFGRMRAEQFIGRVSDGISRLIRSFRETVVSGAAEPPSRV
                     GGAVLEYRLIHLDWPRAGDRLEIRSGLKDADTRTKRMIHWMIDPGTGKAWGTSEAVAI
                     TFDLDARKIVPITEAARLELMKHAKAGLAL"
     gene            complement(1582..2067)
                     /locus_tag="PLO65_02600"
     CDS             complement(1582..2067)
                     /locus_tag="PLO65_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRD27171.1"
                     /translation="MAHSHACDHDHDHRGLVGDALTVGLSTALARCEAAGQRLTPPRR
                     RVLEMLLSAGQPVKAYDLIAAFGDGGAPAKPPTVYRALEFLSRLGLAHRIESLNAYMA
                     CGGGGEGHAAAFLICDCCGATREIAPAGAAEIERLAGAQGYSLTGLMVEAHGLCPDCR
                     S"
     gene            2188..3144
                     /locus_tag="PLO65_02605"
     CDS             2188..3144
                     /locus_tag="PLO65_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRD27172.1"
                     /translation="MQLWLSMAAGLAMLAVGGDLLVRGAASAARRLGVSPLLIGLTVV
                     GFGTSTPELVTSLQAAFAGSPGIAIGNVVGSNISNVLLILGVTALVAPLAVDQRAFRR
                     DAIMLVLATGGLAWAILQGRLTREMGAVSLVLLGVYLTVVWLSERRRPDPEGVRIEAS
                     AREPTKAAASLWIALPLAAAGIAITVYGAQLLVGGAITVAHQLGVSDTVVGLTIVALG
                     TSLPELVTSLVAALRRQGEVALGNVVGSNLYNILGILGITAAIKPVDVPPVIARLDVW
                     VLVAATAAMILFARRLGKVTRGEGLLLLTGYAAYIGWLVWTA"
     gene            complement(3145..3636)
                     /locus_tag="PLO65_02610"
     CDS             complement(3145..3636)
                     /locus_tag="PLO65_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27173.1"
                     /translation="MRLMIIATAVAAVWLAACGKKDDDGKPKVAATPGGYTVTTGDGS
                     ATVSTGAQAARHAAAGLPDFAPLFPGANVENAVTSVSSDDGKSEGATVTYTVDAAPDK
                     IIAFYKQKAEAAGFKSVMDANMGAALMFAASDEASNRALQVIASGKGASTSVVVTWAR
                     PRT"
     gene            complement(3708..4757)
                     /locus_tag="PLO65_02615"
     CDS             complement(3708..4757)
                     /locus_tag="PLO65_02615"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HRD27174.1"
                     /translation="MNAPVQNNTKAQLLANVVEHVDITSFDARPIIDSMRKMSFSSRD
                     TARAADIFNMALEDKACSPWLILAGSTSAGGCMHVYRDMVKFGMVDAIVATGASIVDM
                     DFFEALGFKHYQAAGPVDDNVLRENYIDRIYDTYIDEEELQACDHTILEIANRLEPRG
                     YSSREFIWEMGKWLSEGNARKPGSLIQTAYEEGVPIFCPAFVDSSAGFGLVKHQKERA
                     AAGKPYLMLDAIADFRELTDIKIAAGVTGLFMVGGGVPKNFAQDTVVCAEILGVEAEM
                     HRYAVQITVADVRDGACSSSTLKEAASWGKVQTTHEQMVFAEATTVVPLIGSDAYHRG
                     AWKTREKRRWARLFA"
     gene            complement(4995..6260)
                     /locus_tag="PLO65_02620"
     CDS             complement(4995..6260)
                     /locus_tag="PLO65_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRD27175.1"
                     /translation="MRTYHTPLDLVRERSPDRPVALVRPAPVAVAARWFQDNLKADVF
                     YAVKANPSRWVIEALVGAGVTGFDVASLREIELVRSVSPDARLAFMHPVKSRAAITRA
                     YFDHGLRTFSLDCHDELDKILDATGQASDLNLVVRMAVSADGAAYSLSGKFGIAPEQA
                     SSLLLATRQAVKDGLMGVSFHVGSQCMRPTAYQAAMAQVGRSISRAGVIVDIVDVGGG
                     FPSVYPGMVPPDMSEYADAIHRGFNEMPVSETTELWCEPGRALVAESSSVLCRVDLKK
                     GDALYLNDGSYGSLFDATHSRWPFPTRLVRDGAAATELKPFRFYGPTCDSIDHMPGPF
                     WLPADVREGDFIEVGMLGAYGVAMSTGFNGYGEHEVAMVDDAPMASLYGLAPRSIPTV
                     RTTAEEQARKVVRLSRPKGKAGVRKQRRR"
     gene            6540..7334
                     /gene="phnC"
                     /locus_tag="PLO65_02625"
     CDS             6540..7334
                     /gene="phnC"
                     /locus_tag="PLO65_02625"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRD27176.1"
                     /translation="MTTDAVLSIRSVSKTFGSRRALDGVSLDVARGEMVALIGPSGSG
                     KSTLLRSVDGLQTIDPGDGVIEAFGGKVQARGRLSEKVRETRIRIGFIAQQFNLVGRL
                     SLFTNVALGSLGRIGFLQGVLGRWPEETRMAAMQALHRVGVSDYAGQRANTLSGGQQQ
                     RGAIARALVQKAKIILADEPVASLDPVSARKVMEILRELNRKDGLTVIVTLHQVDYAL
                     KYCDRVVALKAGRMVYDGPAAGLKRDKLIDIYGPEFEDVFWEGAPA"
     gene            7331..8356
                     /gene="phnD"
                     /locus_tag="PLO65_02630"
     CDS             7331..8356
                     /gene="phnD"
                     /locus_tag="PLO65_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD27177.1"
                     /translation="MILRRSLLALGAVAALALAGCGEKAATGAAQDTINFSILNTESA
                     QNMESYWAPILADMEKQTGLKVKPFFSGSYSALIEAMRFKQTDLGWFSNQSGLEAVRR
                     SNGEVFARTFDPSGKDGYRSVIIVPANSPIRTIDDLLKCDRTLNFGMGDKKSTSGTLA
                     PMTYVFIPAGKKPETCFKTVLSASHQANLFAVANGRLDASTNNSTALSLNKGRGEGQA
                     DRVRVIWQSDLLPEDPIVWRKDLDPVIKEKLRQFFLTYGQGTGPEAERQRANLARLSI
                     GGFLPADDSHLLVVRKMEALENLGLAREGGDPAKVAAAQAALDDIQAQLDKKAATTAA
                     APPPPAG"
     gene            8364..9200
                     /gene="phnE"
                     /locus_tag="PLO65_02635"
     CDS             8364..9200
                     /gene="phnE"
                     /locus_tag="PLO65_02635"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRD27178.1"
                     /translation="MTSTDTLQPPRRPLSAQLFDLALWGGVGVLLVISFGPAEIGKFP
                     MLFSSESRMGEFINGFFQPFRFWDPTWTMSDLTENAWGLYVSKMWQTIQMALWGTFLA
                     ILVAIPFGLLGASNITPVWIQQPVRRVLDLIRSIPDLVVAVIFITAVGLGPFAGVMAM
                     MFNTGGVLAKLFSEAVESIDKGPVEGVRATGAGRLQEIVWGVIPQVAPLWTSYGLYRF
                     ESNSRAATVLGLIGAGGIGQVLIDSTNTFSYGQTGCIVLVIVVAVSAIDLLSQAIRSR
                     LL"
     gene            9390..10607
                     /locus_tag="PLO65_02640"
     CDS             9390..10607
                     /locus_tag="PLO65_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD27179.1"
                     /translation="MNTKTSLVCGSALGALMALALASGASAQETTTAWKGAPQFQNDS
                     FTFKVRGRAYIDAVNQDVDRETGADYSSQVTRTRTVRLGVEGTWNQNWAYKAEVQVVG
                     GSAAWEDLILEYKPSDNASIMVGNFKTVSLENVSSSRYITFMERGSYNDVLGVSRVMT
                     VAAKLNGENWTALAAVSGDSINSADPVVIGNDGGSEQASIQGRFTWAPVMTDTNLVHL
                     GVWARKRDRGDQGAFSYAVRNNTNYGAQFTSSGGIGDSDTQIAGEALWIAGPFSLQGE
                     YANIDIDRTNGLDQNARAYYLAASWFVTGEKRNMEVKKGELGRTKILNPVTAGGPGAF
                     ELAVRYDSTDLTDITGVGTAGEYSAWTLGANWYPFPYVRFMANYSMSQNDNPALGADV
                     DVNTLQFRAQFDF"
     gene            10633..11670
                     /locus_tag="PLO65_02645"
     CDS             10633..11670
                     /locus_tag="PLO65_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD27180.1"
                     /translation="MKKILGTVAAVAVMTVASQAHAARDYVWAAGSSTVFPFSTRVAE
                     NFARKTGSKAPKVESLGTGGGFKLFCGGTGEGFPDIANASRPMKKSEFDACAAKGVND
                     IIAIKIGFDGIVIATDKDGADYAFKIEHLYLGLSAKVLRYGKFVPNPYKAWDEIGAGL
                     PGNRIVVYGPPPTSGTRDAFLELGIEEGARRFPLLAELRSNNEKGFKGLVDPLRADGW
                     IDAGENDNAIVQTLTRTPGALGVFGYSFLEENGDKVKGASVNGVFPTATTIANGSYPL
                     SRSLYIYVKKSNLGVTPGLREFVEEFLSDAASARGGYLQSRGLIPLPPAQHEAGKAAF
                     KAGTAMARPAS"
     gene            complement(11748..>11913)
                     /locus_tag="PLO65_02650"
     CDS             complement(11748..>11913)
                     /locus_tag="PLO65_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRD27181.1"
                     /translation="LDRTAVRAFAEKFSWRACAEQFVRNLEPYPEPEKTRFWRRLRRL
                     ARLRRRPKAA"
BASE COUNT         1859 a         4144 c         4015 g         1895 t
ORIGIN      
        1 gccgccgagc ggcccgacgc ggcgcgccgg ctggtgctgt tcgacccggt ggtgatgacc
       61 gccgagcgcg tggccgctgt tggcccggac gggctgaagg cctcgcccct ggccgtcggg
      121 gcccgccgtc gccgcgcgac ctttcccgac cgcgaagcgg ccttccaggc ctacagcggc
      181 cgcggcgcct tccgcacctg gtccgacgcc gaccttcgcg gctatctggc cgacggtctg
      241 ctcgagggcg acgacggcca ggtccatctt tcctgccggc cggactggga ggcggccaat
      301 ttcgccgccc atcaccacga cacgatcggc gccttcgccc gcgtcgtcgc gccgatccgc
      361 atcctcaagg ccgagatcgg ctccacctgc gggctcgagg cccacgaggg cgaggtgctg
      421 gccggcggcg acgtgcggat ggagaccgtt cccgggacca gtcacttcct gccgatggaa
      481 cgccccgacc tggtgcggag cgctctgatg gcggcggcgg gctgattgcg tgcttcgaca
      541 cggcgctttg cgcccgctca ggatgacgtg aacggcaggg tttcgacact ctgcgtcatg
      601 gtgagcagca ccggcagggt gcgtgtcgaa ccacgcacgc ctcctacagc gccaggccag
      661 ccttggcgtg cttcatcagc tccagcctcg cggcctcggt gatcggcacg atcttgcggg
      721 cgtcgaggtc gaaggtgatg gccacggcct cgctcgtgcc ccacgccttg ccggtcccgg
      781 gatcgatcat ccagtggatc atccgcttgg tgcgggtgtc ggcgtccttc aggcccgagc
      841 ggatctccag ccggtcgccg gcgcgcggcc agtccaggtg gatcagccgg tattccagca
      901 cggcgccgcc gacccggctc gggggctcgg cggccccgct gaccacggtc tcgcgaaagc
      961 tgcggatcag ccgcgaaatg ccgtccgaca cccggccgat gaactgctcg gcccgcatgc
     1021 ggccgaacag gtcgcagtcg gccggcgaga ccgcgccctg ggcgatgcgg accagtccca
     1081 gctcctcggc ccgcgcgagg ccggcgatcg aggcggtggg cgccagcgaa atgctgcgcg
     1141 ccattgcctg gtccggcagg ccgatggtca gggcggcggc ctggtccagc gtgcgccgcg
     1201 accagggaaa cggtcgtccc tcggcgggcg tcacatgggc caccacggtc tggaagctgg
     1261 ccgccagggc gccggaatcc gagtgccaca ggagcatcag cagccgcgcc tcgtcctcgc
     1321 ccatctccag cacgccgccg gtcatgtgca gggccgcgcc ggcgtgcgcc tcgcgcagga
     1381 agcggatgtg ctgctcgcgg accatcaggg tcgagccggc atgggcggaa aaggcgtgcg
     1441 gcaggccgag ggcggcggcc agcccgacca gcccctcgat cgccttggtg acgtagaagc
     1501 gcacattcat gtgccccatc tcgtcacatt cccaggtgtt gacgccgccg cgccagatct
     1561 ccaccccgtc cgccacgccg gtcaggaccg gcagtccggg cagaggccgt gcgcctcgac
     1621 catcaggccc gtcaggctgt agccctgggc gccggcgagg cgttcgatct cggcggcgcc
     1681 ggccggcgcg atctcgcggg tcgcgccgca acagtcacag atcaggaagg ccgcggcatg
     1741 gccttcgccg ccgccgccgc aggccatgta ggcgttgagg ctctcgatgc gatgggccag
     1801 gcccagtcgg gacagaaact ccagcgcccg gtagaccgtc ggcggcttgg cgggcgcgcc
     1861 gccgtcgccg aacgcggcga tcaggtcgta ggccttcacc ggctggccgg cggacagcag
     1921 catttccagc acgcgccggc gcggcggagt caggcgctgc ccggccgcct cgcagcgcgc
     1981 cagagccgtc gacaggccga ccgtcagggc gtccccgaca aggccgcggt ggtcatggtc
     2041 gtgatcgcag gcgtgggaat gggccatgcc tgagaggtag aggcggcgct ccgccgaagc
     2101 aagccgggcg gcggtccggc ggcgccggtt ttccttgcat gcggccctgt ccgcgccatg
     2161 ctgtcgccgg gctattcggg aggctgaatg caactctggc tgagcatggc ggccgggctg
     2221 gcgatgctgg cggtcggcgg tgacctgctg gtgcgcgggg cggccagcgc ggcgcggcgg
     2281 ctgggcgtca gtccattgct gatcggcctg accgttgtcg gcttcgggac ctccacgccg
     2341 gaactggtca ccagcctgca ggcggccttc gccggctcgc cgggtatcgc catcggcaac
     2401 gtcgtcggct ccaacatctc caacgtgctg ctgattctcg gcgtcaccgc cctggtcgcg
     2461 ccgctggcgg tcgaccagcg cgccttccgg cgggacgcga tcatgctggt gctggcgacc
     2521 gggggcctgg cctgggcgat tctccagggt cgactgaccc gcgagatggg tgcggtctcg
     2581 ctggtcctgc tgggcgtgta tctgacagtg gtctggctgt ccgaacgccg ccggcccgac
     2641 ccggaaggcg tccggatcga agcgtccgcc cgcgagccga cgaaggccgc ggccagtctc
     2701 tggatcgccc tgcccctggc ggcggcgggg atcgccataa ccgtctacgg cgcccagctg
     2761 ctggtcggcg gggccatcac cgtggcccat cagctggggg tttccgacac cgtggtcggc
     2821 ctgaccatcg tggccctggg cacctccctg ccggaactgg tgaccagcct ggtcgcggcg
     2881 ctgcgcaggc agggcgaggt ggccctgggc aacgtcgtcg gctcaaacct ctacaacatc
     2941 ctcggcatcc tcgggatcac cgcggcgatc aagcccgttg acgtgccgcc ggtgatcgcc
     3001 cgcctcgacg tctgggttct ggtcgcggcg acggcggcga tgatcctgtt cgcccgacgc
     3061 ctgggcaagg tgacccgcgg cgagggcctg ctgttgctga cgggctatgc cgcctacatc
     3121 ggctggctgg tctggaccgc ctgatcaggt cctgggccgg gcccaggtca ccaccacact
     3181 ggtcgaggcg cccttgccgg aggcgatcac ctggagcgcg cgattgctcg cctcgtcgct
     3241 ggcggcgaac atcagggccg cccccatgtt ggcgtccatc acggacttga agccggcggc
     3301 ctcggccttc tgcttgtaga aggcgatgat cttgtccggc gccgcgtcaa ccgtataggt
     3361 gaccgtcgcc ccctcgctct tgccgtcgtc gctggagacc gaggtcacgg cgttctcgac
     3421 attggccccc ggaaacagcg gagcgaagtc gggcaggccg gcggccgcgt gacgggctgc
     3481 ctgggcgccg gtgctgaccg tggccgaacc gtcgcccgtg gtcaccgtgt agccaccggg
     3541 cgtggccgcg accttcggtt tgccgtcgtc gtccttcttg ccgcaggcgg ccagccaaac
     3601 ggcggcgacc gccgtggcga tgatcatcag acgcatggcg acccccaaag aaaaaccgcg
     3661 cgaagcctga gcctcgcgcg gtccgtcatt tttcgcaatg atctcgatca ggcgaacagc
     3721 ctggcccagc ggcgcttctc gcgggtcttc caggcgccgc gatgataggc gtccgagccg
     3781 atcagcggca ccaccgtcgt ggcctcggcg aagaccatct gctcgtgcgt ggtctgcacc
     3841 ttgccccagc tggccgcttc cttgagggtc gaggacgagc aggcgccgtc gcgcacgtcg
     3901 gccacggtga tctgcacggc gtagcggtgc atctccgcct cgacgccgag gatctcggcg
     3961 cagaccacgg tgtcctgggc gaagttcttc ggcaccccgc cgccgaccat gaacaggccc
     4021 gtgacgccgg cggcgatctt gatgtcggtc agttcgcgga agtcggcgat ggcgtcgagc
     4081 atcaggtagg gcttgccggc ggcggcgcgc tccttctggt gcttgaccag gccaaagccg
     4141 gccgagctgt cgacgaaggc cgggcagaag atcggcacgc cttcttcata ggcggtctgg
     4201 atcaggctgc cgggcttcct ggcattgccc tcgctcagcc acttgcccat ctcccagatg
     4261 aactcgcgcg aggagtagcc gcgcggctcc aggcggttgg cgatctccag gatggtgtgg
     4321 tcgcaggcct ggagctcttc ctcgtcgatg taggtgtcgt agatgcggtc gatgtagttc
     4381 tcgcgcagta cgttgtcgtc gaccgggccg gcggcctggt agtgcttgaa gccgagggcc
     4441 tcgaagaaat ccatgtcgac gatgctggcg ccggtggcga cgatcgcgtc gaccatgccg
     4501 aacttcacca tgtcccggta gacatgcatg cagccgccgg cgctcgtcga tccggccagg
     4561 atcagccagg gcgagcaggc cttgtcttcc agggccatgt tgaagatgtc ggccgcccgg
     4621 gcggtgtcgc gcgagctgaa gctcatcttg cgcatcgaat cgatgatcgg acgggcgtcg
     4681 aaggacgtga tgtccacatg ctcgacgaca ttggcgagca gctgggcctt ggtgttgttt
     4741 tgaacgggag cgttcatctc gcgggtttcc ctttggtttg agcgcccgtc gaatacgagg
     4801 atcgggctgg gacggagcga ccagcgcgac ctttctcccg gagggagaag gaggggcccg
     4861 cccgtctgaa cgggcgggag gatgaggggt tcaggtgttc gacgattcaa cgccgtaccc
     4921 cctcaccctt tcgaccttgc gcatcgctgc gctctgcggg tctcaagccc tctcccgctg
     4981 ggagagggac gagactaccg ccgccgctgc ttgcggacgc ccgccttacc cttcggacgt
     5041 gacaggcgga cgaccttgcg ggcctgctcc tcggccgtgg tgcgcacggt cgggatggag
     5101 cggggcgcca ggccatagag cgaggccatc ggggcgtcgt cgaccatggc gacctcatgc
     5161 tcgccatagc cgttgaagcc ggtcgacatg gccacgccat aggcgcccag catgccgacc
     5221 tcgatgaagt cgccctcgcg cacgtcggcc ggcagccaga acggtcccgg catgtggtcg
     5281 atcgaatcgc aggtcggccc gtagaagcgg aagggcttca gctcggtcgc ggccgcgccg
     5341 tcccgcacca gcctggtcgg gaacggccag cgcgaatgcg tggcgtcgaa cagcgagccg
     5401 taggagccgt cgttcagata cagggcgtcg cccttcttca gatcgacccg gcacagcacc
     5461 gacgaggact ccgcgaccag cgcccggccg ggctcgcacc acagctcggt ggtctcgctg
     5521 accggcatct cgttgaagcc gcgatggatg gcgtcggcgt attcgctcat gtccggcggg
     5581 accatgcccg ggtagaccga cgggaaaccg ccgccgacat cgacgatgtc gacgatcacc
     5641 ccggcgcggc tgatcgaacg gccgacctgg gccatggccg cctggtaggc ggtcgggcgc
     5701 atgcactggc tgccgacatg gaagctgact cccatcagcc cgtccttgac cgcctgtcgg
     5761 gtggccagca gcagcgaaga cgcctgctcc ggcgcaatgc cgaacttgcc tgacaggctg
     5821 taggcggcgc cgtcggccga gaccgccatg cgcacgacca ggttcaggtc gctggcctgg
     5881 ccggtggcgt cgagaatctt gtccagctcg tcgtggcagt cgagcgagaa ggtccgcagg
     5941 ccgtgatcga aataggcccg ggtgatcgcc gcgcggctct tgaccggatg catgaaggcc
     6001 agccgagcgt ccgggctcac cgaccgcacc agctcgattt ccctcagcga ggcgacgtca
     6061 aatccagtga cgcccgcgcc gaccagggcc tcgatgaccc agcgcgacgg gttcgccttc
     6121 accgcataga agacgtcagc tttcagattg tcctggaacc agcgcgccgc tacggcgacg
     6181 ggggcaggtc gcacaagtgc gacaggccta tccggcgacc gctcacggac caggtccagg
     6241 ggagtatggt acgtgcgcaa ttcacgtagc cccctgtaaa ttgttaaacc cagaccggcg
     6301 ttagcagcgc agttggacac ggggtccgcc ggaagcgcgc gagatagggt ctgttgattc
     6361 agatgtaaag aggcatttgt cgcgtggcgc gagcggtcgg gcgcgcattt cccgccaggg
     6421 gtggcctgac agcccttccc agccggtccc ggggagagat atgccttccc gtcacgaaac
     6481 tttcgcacgc cccttccatg agggacgggc ttctgtgtta ggactctcgc cgaaaagcca
     6541 tgacgacaga cgccgtcctc tcgatccgct cggtctccaa gacctttggt tcccgccgcg
     6601 cgctcgacgg ggtctccctt gacgtcgcgc gcggggagat ggtcgcgctg atcggtccgt
     6661 ccgggtcggg taaatcgacc ctgttgcgct cggtggacgg cctgcagacg atcgatccgg
     6721 gcgacggcgt gatcgaggcg ttcggcggca aggtacaggc ccgcggacga ctgagcgaga
     6781 aggtccgcga gacgcgcatc cgcatcggct tcatcgccca gcagttcaac ctggtgggcc
     6841 ggctcagcct gttcaccaac gtcgcgctcg ggtctctggg ccgcatcggt ttcctccagg
     6901 gcgtgctcgg ccgctggccg gaggagacca ggatggcggc catgcaggcg ctgcaccggg
     6961 tcggggtctc cgactacgcc ggccagcggg ccaacaccct ctccggcggc cagcagcagc
     7021 ggggggccat cgcccgcgcc ctggtccaga aggccaagat catcctcgcc gacgaaccgg
     7081 tggcctcgct ggatccggta tccgcccgca aggtcatgga gatcctgcgc gaactgaacc
     7141 gcaaggacgg gctgaccgtc atcgtcaccc tgcaccaggt cgactacgcc ctgaagtact
     7201 gcgaccgggt cgtggcgctg aaggccggca ggatggtcta tgacggtccc gccgccggcc
     7261 tcaagcgcga caaactcatc gatatctacg gcccggaatt cgaagacgtg ttctgggaag
     7321 gagcccccgc atgattctcc gccgctctct tctggccctc ggggccgtcg cggccctggc
     7381 gctcgccggc tgtggcgaga aggccgccac cggcgccgcc caggacacga tcaatttctc
     7441 gatcctcaac accgagtccg cccagaacat ggagtcctac tgggctccca ttctggccga
     7501 catggaaaag cagaccggcc tgaaggtgaa gcccttcttc tccggcagct acagcgcgct
     7561 gatcgaggcg atgcggttca agcagaccga cctgggctgg ttctccaacc agtcgggcct
     7621 ggaggccgtg cgccgctcga acggcgaggt cttcgcccgc accttcgacc cgtccggcaa
     7681 ggacggctat cgctcggtca tcatcgtgcc ggcgaacagc ccgatccgta cgatcgacga
     7741 cctgctcaag tgcgaccgga ccctgaactt cggcatgggc gacaagaagt cgacctccgg
     7801 caccctggcg ccgatgacct acgtcttcat tcccgccggc aagaagccgg agacctgctt
     7861 caagacggtc ctgtcggcca gccaccaggc caacctgttc gcggtggcca atggccgcct
     7921 cgacgcctcg accaacaatt ccacggccct gtcgctgaac aaggggcgtg gcgagggcca
     7981 ggccgaccgg gtgcgggtga tctggcagtc ggaccttctg ccggaggatc cgatcgtgtg
     8041 gcgcaaggac ctcgatccgg tgatcaagga gaagctgcgc cagttcttcc tcacctatgg
     8101 ccagggaacg ggccccgaag ccgaacgcca gcgggccaac ctggcccggc tgagcatcgg
     8161 cggcttcctg ccggccgacg acagccatct gctggtggtg cgcaagatgg aggcgctcga
     8221 gaacctcggc ctcgcccggg agggcggcga tccggccaag gtcgccgcgg cccaggccgc
     8281 gctggacgac atccaggccc agctcgacaa gaaggcggcg accacggcgg ctgcgccgcc
     8341 cccgcccgcc ggctgatccc cgcatgacct cgacggacac cctgcaaccg ccgcgccggc
     8401 ccctgtcggc ccagctgttc gacctcgccc tctggggcgg cgtcggcgtg ctgctggtga
     8461 tcagtttcgg accggccgag atcggcaagt tcccgatgct gttcagcagc gaaagccgga
     8521 tgggcgagtt catcaacggc ttcttccagc cgttccggtt ctgggacccg acctggacca
     8581 tgtccgacct gaccgagaac gcctgggggc tctacgtcag caagatgtgg cagacgatcc
     8641 agatggcgct gtggggcacc ttcctcgcca tcctggtggc catcccgttc ggcctgctcg
     8701 gcgcgagcaa catcacgccg gtctggatcc agcagccggt gcggcgcgtg ctggacctga
     8761 ttcgctccat tcccgacctg gtcgtggcgg tgatcttcat caccgcggtc ggtctcgggc
     8821 cgttcgccgg ggtcatggcg atgatgttca ataccggcgg ggtgctggcc aagctgttct
     8881 ccgaggcggt cgagtcgatc gacaagggcc cggtcgaggg ggtgcgcgcc accggtgccg
     8941 gccgcctgca ggaaatcgtc tggggcgtca tcccccaggt cgccccgctg tggaccagct
     9001 acggcctcta ccgcttcgaa tccaacagcc gggcggcgac cgtgctgggc ctgatcggcg
     9061 ccggcggcat cggccaggtg ctgatcgatt cgaccaacac cttcagctac ggccagaccg
     9121 gctgcatcgt gctcgtcatc gtcgtggcgg tgtcggccat cgacctgctg tcccaggcca
     9181 tccgcagccg cctgctgtga gcgcggggac acgtacctga agaaggtgca tgtccccaat
     9241 tcctccgtat cctgaccgcc tgtcaccaaa tcgtcgcgcc gctgtcgcgg aaccttgcct
     9301 gacctgtcgc cggaccgtcg cggcccgccc ctaggagtcc ggccgaaccc gacgcgaggg
     9361 gcgtggggta tcgggacaag gatccgaaca tgaacacgaa gacctcgctg gtttgcgggt
     9421 ccgctctggg cgcgctgatg gcgctggccc tggcgtcggg cgcctccgct caggaaacca
     9481 ccaccgcctg gaagggcgct ccgcaattcc agaacgacag cttcaccttc aaggtccgcg
     9541 gtcgcgccta catcgacgcc gtgaaccagg acgtcgatcg cgagaccggc gcggactaca
     9601 gctcgcaggt cacccgcacc cgcacggtgc gcctcggcgt cgagggcacc tggaaccaga
     9661 actgggccta caaggccgag gtgcaggtcg tcggcggctc ggccgcctgg gaagacctga
     9721 tcctcgagta caagccgtcc gacaacgcct cgatcatggt cggcaacttc aagaccgttt
     9781 cgctcgagaa cgtgtcctcg tcgcgctaca tcaccttcat ggagcgcgga tcgtacaacg
     9841 acgtgctcgg cgtcagccgc gtgatgaccg tcgccgccaa gctgaacggc gaaaactgga
     9901 cggcgctggc cgccgtgtcg ggcgattcga tcaacagcgc cgacccggtc gtgatcggca
     9961 acgacggcgg cagcgagcag gcttccatcc agggccgctt cacctgggcg ccggtgatga
    10021 ccgacaccaa cctggtccac ctgggcgtct gggcccgcaa gcgcgaccgc ggtgatcagg
    10081 gcgcgttcag ctacgcggtg cgcaacaaca ccaactacgg cgcccagttc acctccagcg
    10141 gcggtatcgg cgacagcgac acccagatcg ccggcgaagc cctgtggatc gccggtccct
    10201 tctcgctgca gggcgagtac gccaacatcg acatcgaccg caccaacggc ctcgaccaga
    10261 acgccagggc ctactacctg gccgccagct ggttcgtgac cggcgaaaag cgcaacatgg
    10321 aggtgaagaa gggcgaactg ggccgcacca agatcctcaa tccggtgacg gccggcggcc
    10381 ccggtgcatt cgagctcgcg gttcgctatg acagcaccga cctgaccgat atcaccggcg
    10441 ttggcacggc cggcgagtat tcggcctgga ccctcggcgc caactggtac ccgttcccct
    10501 atgtccgctt catggccaac tacagcatgt cgcagaacga caaccccgcc ctcggcgccg
    10561 acgtcgacgt caacaccctg cagttccgcg cccagttcga tttctgatcg ccttcaccaa
    10621 ggacatcctc ccatgaagaa aatcctcgga acggtcgccg ccgtcgcggt catgaccgtc
    10681 gcgtcccagg cccatgcggc ccgggactat gtctgggccg ccggttcctc gaccgtgttc
    10741 cccttcagca cccgcgtggc ggagaacttc gcccgcaaga ccggctccaa ggcgcccaag
    10801 gtcgaatccc tgggcaccgg cggcggcttc aagctgttct gcggcggcac cggcgaaggc
    10861 ttccccgaca tcgccaacgc ctcgcggccg atgaagaagt ccgaattcga cgcctgcgcc
    10921 gccaaggggg tgaacgacat catcgccatc aagatcggct tcgacggcat cgtcatcgcc
    10981 accgacaagg acggcgccga ctacgccttc aagatcgagc atctgtatct gggcctgtcg
    11041 gccaaggtgc tgcgctacgg caagttcgtg cccaacccct acaaggcctg ggacgaaatc
    11101 ggcgcgggcc tgccgggcaa ccgcatcgtg gtctacggcc cgccgcccac gtcgggcacc
    11161 cgtgacgcct tcctcgagct cggcattgaa gagggcgcgc gccgcttccc gctgctggcc
    11221 gagctgcgct cgaacaacga gaagggcttc aagggcctgg tcgacccgct gcgcgcggac
    11281 ggctggatcg acgcgggcga gaacgacaac gccatcgtcc agaccctgac ccgcacgccg
    11341 ggcgccctgg gcgtgttcgg ctactccttc ctcgaggaga acggcgacaa ggtgaagggc
    11401 gcctcggtca acggcgtctt cccgaccgcc acgaccatcg ccaacggcag ctacccgctg
    11461 tcgcgctcgc tctacatcta cgtcaagaag tccaacctgg gcgtgacgcc gggcctgcgc
    11521 gagttcgtcg aggagttcct ctccgacgcg gcgtcggcgc gtggcggcta tctccagagc
    11581 cgcggcctga tcccgctgcc gcccgcccag cacgaagccg gcaaggccgc gttcaaggcc
    11641 ggcacggcca tggcgcgtcc ggcgagctag atctctcccg ccagcgcgac gaccggcgcc
    11701 gcggctcgaa agggccgcgg cgtttttcgt gggagaattg gccttgtcta ggccgctttc
    11761 ggccgccgcc gcagtcgcgc cagacgccgc agacgccgcc agaagcgcgt cttttccggc
    11821 tcgggatagg gctcgaggtt gcgcacgaac tgctcggcgc aggcgcgcca gctgaacttc
    11881 tcggcgaagg cgcgcacggc cgtccggtcc agg
//
LOCUS       DAORNX010000034        11889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4070_length_11889_cov_8.338516, whole genome shotgun sequence.
ACCESSION   DAORNX010000034 DAORNX010000000
VERSION     DAORNX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_11889_cov_8.338516"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1129
                     /locus_tag="PLO65_02655"
     CDS             233..1129
                     /locus_tag="PLO65_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD27182.1"
                     /translation="MEIGQWKATAELGDVYRKARDLGLETNLAELEAFGFTIIEPEKA
                     APPGFTDRMLEAVLALEARESPDVVDMMNRPQSERPVYGRSLFRIPAKDPVFAEAAVS
                     PVALTLAKYLTGASNHIYNSAALLKRGKAGTTRMHCDSVGFSSPLPPYGATCNTSWIL
                     TDYTEEKGTFFIVPGSHRYCRQPGPMDLPRMMGGPNENDSGIPVIAKAGSLFVFSGNL
                     WHGTYPKEDEDLRVHCAIMYSRNYVYPGESFSDVPDDYIARFGPEFARLMGRKAWQGY
                     GDEGPNVMMLAGVQRAHVTPSA"
     gene            complement(1259..2143)
                     /locus_tag="PLO65_02660"
     CDS             complement(1259..2143)
                     /locus_tag="PLO65_02660"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD27183.1"
                     /translation="MTTEYEYMIYEKPEPGVARIVMNRPQAANAQDTRFLYELNACFD
                     RGAREDDVRVIILAANGKHFSSGHDIRERDHHDNQDLFTPVTNFQQHRRAGAEGRMSR
                     EEELYTGLSERWRNLPKITIAAVQGKCVSGGLLLCWPCDLIIASDDAEFTEVTVAMGI
                     AGVEWFAHPWEMPARKAKEMLLTGDPIGAEEARQLGMVNKVVPRADLPEAALAMARKI
                     AAQPSFATKMAKQAVNGAQDAQGRPAAMQIAFALHQLAHSHCMEVHGLPVDPGGIFGG
                     VKKSYAEGDEPFRAPAAE"
     gene            complement(2170..2589)
                     /locus_tag="PLO65_02665"
     CDS             complement(2170..2589)
                     /locus_tag="PLO65_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27184.1"
                     /translation="MSDKIELGSPEWMAALKGKIEACMAADPGMRFSICEVFTGVPSH
                     LDKHGTGVLAWYCRIADGKLEFEDGEIDDADIKTTTDYDFILPFARMKLTPETMDEYQ
                     RMHEDGLKRGLIKPQGDRSKVPPAFHGMHNELAEVTA"
     gene            complement(2600..3499)
                     /locus_tag="PLO65_02670"
     CDS             complement(2600..3499)
                     /locus_tag="PLO65_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD27185.1"
                     /translation="MEIGDWKSVAGMSETYRRARELGLETNIAELEAFGFTVIEPEKT
                     GAPAGFAERLYEKVLEIGRQEDALAVELNKHADAAKPAYGRQLFHLLARDPIFAEAVM
                     NPVAQTMASYLMGLSYRLFSMVAFVKDGPARSTHMHSDSVGVPSPLPAYGSVCNVSWI
                     LTDYTPENGTLGMVPGSHRLCRHPTDMEQPKFLGGPMDDAMVAPIIARPGSLAVFTGN
                     TWHCTYPKTTDGVRAHIATAYCRNYIAPAEAYDDLPDSAIAPFGPELARLVGRSAWQG
                     YRAEGPKLENMALVRPAYQSQYG"
     gene            complement(3595..4077)
                     /locus_tag="PLO65_02675"
     CDS             complement(3595..4077)
                     /locus_tag="PLO65_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD27186.1"
                     /translation="MTPVDRMLAEQACQKLQMQYGVFADRGEVEGFVGLFTTDASITV
                     PEHPPFTGHDAIRASIQALAATGLTMRHVITNPVIEVTGPDSATGVCYLIVFNSAAPA
                     DPAGTRPAALPATVGEYADRFRRTADGWRFESRVLRRVFRRIDDPMLAAAGQAPQGKA
                     "
     gene            complement(4074..5498)
                     /locus_tag="PLO65_02680"
     CDS             complement(4074..5498)
                     /locus_tag="PLO65_02680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD27187.1"
                     /translation="MKRRYVVGAATAGVLLAGAVAVNVAIGDVVTVFTSGSAKMLCTD
                     IFVAGRDPALAWRQDFKRLSTPGKYLGVVKPKVDLAGKTVTASLWGYKTRAAIFRQGI
                     GCTAAEGASVAALRAQGQGVATALPRPDPDTLWPEGGATQIGHLPPGVDGSALTAAID
                     KVFTESDPARPINTRGVVIIYKGRIIGERYGEGFDTETAHLSNSVAKSVTATLIGILV
                     GQGRLDLNKPAPIAEWGGPGDPRGAITLDNLMRMSSGLQFEESYSKVKSDITMQYVGG
                     DLAGYSAAKPLEVPPGTRWQYSTGTSNILGRIVTEAAGPDLPTAFAFPRKALFEPLGM
                     RTAVFEPDAAGNLVGGSSFYASARDYGRLGLLYLNDGLWDGKRILPEGWVDYVRRPTP
                     GSDGRYGAQFWLRGKHSHDLGIPPDMFNMNGHQGQHVVIIPSRDLVVVRVGLSEFDTW
                     DATAFVADVLRALPAPSAGKGRPV"
     gene            complement(5500..7128)
                     /locus_tag="PLO65_02685"
     CDS             complement(5500..7128)
                     /locus_tag="PLO65_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HRD27188.1"
                     /translation="MTNAATLAKPPIAYFPEIRTLGDAPRFHGRTRGDKLALWFEGRA
                     TTFAEFDKHTARVANGLLAEGVRRGSRISYLGKNSDRYFEILFGAARIGAVLAPVNWR
                     LAEPEVVYITNDAEAEVLFVDGHCLPLVERIEGQLPTVRKIVTMDPGGRREDYQTWRD
                     RQSDADQAEPVSRDDVAVQLYTSGTTGRPKGVQLAHRNFYAFNEYAATHPGAFGSDMD
                     WNQWEPEDVSLIAMPIFHISGTGWGVVGLYSGATNIVLSEFDNTAVINSIRDFRISKT
                     VLVPTTIQMILDTPGLDPADFASMTDFLYGASAIPLDLLERAIDTLKCGFVQLYGMTE
                     TTGAVTYLPRDDHRAGNPRLRSAGKVIPGAQIRIMDPAGDEVFGPNRVGEICIKSPTT
                     MVGYWKQDAETARTIQADGWVRTGDAGMMDEDGYVFISDRVKDMIVTGGENVYPAEVE
                     NAMYGHPDIAEVAVIGVPDAKWGEAVKAVVVVKPGHAADAASILDFTRQRIAGYKTPK
                     SVDFIDALPRNASGKVLKRDLREPYWAGRDRRVN"
     gene            complement(7125..8783)
                     /locus_tag="PLO65_02690"
     CDS             complement(7125..8783)
                     /locus_tag="PLO65_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRD27189.1"
                     /translation="MTTPLQGLMQDRPLLIASLLTHGATFNGPREIVSRLPGSLARFT
                     FAQAEVRARRLAKGLERLGLRPGDRLASLAWNTHRHFELFYGVPGMGAVLHTLNPRLW
                     EEQLVYIAGHAEDRVLFFDPDLLGLVVRLAPKLPSVEHYVLLGGRDELPAELPFPVIA
                     YEDLLADDDYRWPEFDERTAALLCYTSGTTGMPKGVLSSHRSTVLHTLYSQSANAFGL
                     TPFDSVLLVVPMFHAQGWGLPFSCAIAGAKLVLPGAAPDPRTVVDLLHAEGATLAMGV
                     PTVWTGVFDELDRSGRDLGPLKRALIGGSAVPPAMSQRLRGQYGVAVVTGWGMTELSP
                     LGSFTGSTPEIEALPAERHEAALHGRAGRMLYPVEIRVLGPDGAVLPHDGVSAGDIQV
                     RGPCVASGYYRGEGGDVLTDDGWFPTGDVGTVDEHGAVAITDRLKDLIKSGGEWISST
                     DLEKAACDTPGVAQAAAIAVPHPRWQERPLMLVTASPGVRLDPGAVLETMRRTLARWQ
                     LPDDVVVVDELPLTATGKIDKKALRARHQDHLAGLATGSLEESA"
     gene            complement(8780..10900)
                     /locus_tag="PLO65_02695"
     CDS             complement(8780..10900)
                     /locus_tag="PLO65_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRD27190.1"
                     /translation="MRRTMRHLGAALVMLLLLGLGGAPQAAWARANPPPRAEILWDSW
                     GVPHIFARTDNDLFYALGWATMHNHANVMLRLYGQARGRAAEYWGESYVESDIQVRTL
                     KIGERAERALAQQTPAFRAKLDAFAEGVNAYAAAHPDKIAADVRQVLPVHPVDFMAHQ
                     QRSGVLPFAFAALKGEVDSWMSRGSNAWAIGPKKSASGNAMLVMNPHIPWGDFAFGGL
                     FRVFEVDAKSPSMDFYGAMQVGTPVISGGFNDHLGWTGTVNTLDDVDVYELTLADGGY
                     VYDGAVRPFEETRTSFGVRQPDGTLEDRPLVIRWSVQGPVIGEKPGKALAVRLAAIDM
                     PFQYQQYWDMTVSRSWKQFRKAIARMQFPKQNLVYADAGGNIFYLASGRIPAREDGDY
                     AFWDGIVPGDTSRTLWTDTLPFEALPQTFNPPAGWLQNENEPPWYVTDPATFTPEQFP
                     AWMSPRTLGPRTQRSLRMITARPMMTFDDVVEDKLSTRSELADRLVDDLVAAAKTGGG
                     ERALKAAAVLAAWDREMDGDSRGAILFMAWLGKYKRPYDMDPALFRETWVAARPMATP
                     AGLADPTRAVALLEAAADETIARYGALDVPYGDVYRLRWGGGIDLPGNGATGGYGVFR
                     VTDYLPDADGKRRAFQGDSFVSIMEFSRPVRARVLLSYGNASQPGSPHDGDQLRLYSA
                     KTLRPVWRTRAEIEANLEEREILR"
     gene            complement(10923..11492)
                     /locus_tag="PLO65_02700"
     CDS             complement(10923..11492)
                     /locus_tag="PLO65_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRD27191.1"
                     /translation="MDGIAPQQCDRTGDRALAALYRDNIDRLRAYLNRLLKSEADADD
                     VAQEAFLRLCRAPDFASFNHPRAVLFKTGYRLALNRMRQRRSNPIDRSEPMLEDVVCG
                     RGAAASAEEILIAGEQEAAYGRALESLPNRCREVIELRTVQELSYKEMSETLGLSVST
                     LEKHLAKGKRVCAEVLSAWWAEPMRAEAA"
BASE COUNT         1790 a         3993 c         4142 g         1964 t
ORIGIN      
        1 acgccggccg cggtgcgcat catcgggcgc gtcgccggcg cgggggcggt cgtgccgccc
       61 ggggatgact ggacgtcgct catggatagg ccctccgcca tggagacacc gggcggcccc
      121 tgatccgcca cgcccgaccg gcccgcgcgg cgggcctttc gatggcggaa gccggtcgcc
      181 gccgtgtctc agacgccgac ggcggatgca acggccgcgc agggagaacg ggatggaaat
      241 cgggcaatgg aaggcgaccg ccgaactcgg cgacgtctat cgcaaggcgc gggacctcgg
      301 tcttgagacc aatctggcgg aactggaagc attcggcttc accatcatcg agccggaaaa
      361 ggccgccccg cccggcttca ccgaccgcat gctcgaggcc gtgctggctc tggaggcgcg
      421 cgagtcgccg gacgtggtcg acatgatgaa ccggccccag tcggagcgcc cggtctacgg
      481 tcgcagcctg tttcgtattc cggccaagga tccggtgttc gccgaagcgg cggtcagccc
      541 ggtagcgctg accctcgcca agtacctgac gggcgcgagc aaccacatct acaattccgc
      601 cgccctcctg aagcgtggca aggccggcac gacacgcatg cactgcgact cggtgggctt
      661 ctcctcgccg ctgccgccct atggcgcgac ctgcaacacc agctggatcc tgaccgacta
      721 caccgaggag aagggcacct tcttcatcgt gcccggcagt caccgctatt gccgtcagcc
      781 ggggccgatg gatctgccca ggatgatggg cggccccaac gagaacgact cgggcattcc
      841 cgtgatcgcc aaggccggct cgctgttcgt cttcagcggc aatctctggc acggcaccta
      901 tccgaaggag gacgaggatc tgcgcgtgca ctgcgcgatc atgtattcgc gcaactacgt
      961 ctatccgggc gagagcttct cggatgttcc cgacgactac atcgcccggt tcggaccgga
     1021 gttcgcccgg ctgatgggcc gcaaggcctg gcagggctac ggtgacgaag ggcccaacgt
     1081 gatgatgctg gccggcgtcc agcgcgcgca tgtgacgccg tcggcataga gactacgccg
     1141 gccccgggga caggcgttga actgaatgtc tccattcgcc cggcaaagaa acgccgcgga
     1201 gcctggctcc gcggcgtcgt gcgatgaagg cgcccgaagg tatcgaggga ggttcggctt
     1261 actcggccgc cggggcccgg aagggctcat cgccctccgc gtaggatttc ttcacgccgc
     1321 cgaagatgcc gccaggatcg accggcaggc cgtgcacttc catgcagtgg ctgtgcgcaa
     1381 gctggtgcag ggcgaaggcg atctgcatcg cggccggccg gccctgggcg tcctgggcgc
     1441 cgttgaccgc ctgtttggcc atcttggtgg cgaagctcgg ctgggcggcg atcttgcgcg
     1501 ccatggccag ggccgcctcg ggcaggtcgg cccgcggcac gaccttgttg accatgccca
     1561 gctggcgggc ttcctcggcg ccgatcgggt cgcctgtcag cagcatctcc ttggccttgc
     1621 gggccggcat ctcccaggga tgggcgaacc attcgacgcc ggcgatgccc atggccacgg
     1681 tgacctcggt gaattcggcg tcgtcgctgg cgatgatcag gtcgcagggc cagcagagca
     1741 gcaggccgcc cgagacacat ttcccctgca ccgccgcgat ggtgatcttg ggcaggttgc
     1801 gccagcgctc gctgagaccc gtgtacagct cctcctcgcg cgacatgcga ccctcggcgc
     1861 cggcgcgacg gtgctgctgg aagttggtca ccggggtgaa caggtcctgg ttgtcgtggt
     1921 ggtcccgctc gcggatgtcg tggccggacg agaagtgctt gccgttggcg gccaggatga
     1981 tcacccgcac atcgtcctcg cgggccccgc ggtcgaagca ggcgttcagc tcatagagaa
     2041 accgcgtgtc ctgggcgttg gcggcctggg ggcggttcat cacgatccgc gcgacgcccg
     2101 gctcgggctt ctcgtagatc atgtactcgt attcggttgt catcggcgtt cctcgggtgg
     2161 gctccgcctt caggcggtga cctcggccag ttcgttgtgc atgccgtgga aggcgggcgg
     2221 caccttgctg cggtcgccct gcggcttgat cagcccccgc ttgaggccgt cctcgtgcat
     2281 gcgctggtac tcgtccatcg tctccggcgt cagcttcatg cgcgcgaacg gcaggatgaa
     2341 gtcgtagtcc gtcgtggtct tgatgtcggc gtcgtcgatc tcgccgtcct cgaactccag
     2401 cttgccgtca gcgatgcggc agtaccaggc gagaacgccg gtgccgtgct tgtcgaggtg
     2461 gctgggcacg cccgtgaaca cctcgcagat gctgaagcgc atgcccggat cggcggccat
     2521 gcaggcctcg atcttgccct tgagggccgc catccattcg gggctgccca gttcgatctt
     2581 gtcgctcatg ccgcgctcct cagccgtact ggctctgata ggccgggcgc acgagcgcca
     2641 tgttttccag cttcggccct tcggcgcgat acccctgcca ggcgctgcgg ccgacgaggc
     2701 gcgcgagttc cggcccgaac ggggcgatgg ccgagtcggg caggtcgtca taggcctcgg
     2761 cgggggcgat gtagttgcga caataggccg tggcgatgtg ggcgcgcacc ccgtccgtgg
     2821 ttttggggta ggtgcagtgc caggtgttgc cggtaaacac cgccagggat cccggcctgg
     2881 cgatgatcgg ggcgaccatg gcgtcgtcca tcggaccgcc gaggaacttc ggctgctcca
     2941 tgtcggtcgg gtgacggcac aggcggtggc tgcccgggac catgcccagc gtgccgttct
     3001 cgggggtgta gtcggtgagg atccagctga cgttgcagac gctgccatag gccggcagcg
     3061 gcgagggcac gccgaccgaa tcgctgtgca tatgggtcga tcgggccggg ccatccttga
     3121 cgaaggcgac catgctgaag agccggtagc tcaggcccat cagatagctg gccatggtct
     3181 gggccaccgg attcatgacc gcctcggcga agatcggatc gcgcgcgagc aggtggaaca
     3241 actggcggcc ataggccggc ttggccgcgt cggcgtgctt gttcagctcc acggccagcg
     3301 cgtcttcctg ccggccgatc tcgaggacct tttcgtacag tcgctcggcg aaccccgccg
     3361 gagcgccggt cttttcgggc tcaatgacag tgaatccaaa ggcctcgagc tcggcgatgt
     3421 tggtttcgag gcccagttca cgcgcccggc gataggtctc gctcatgccg gcgacagact
     3481 tccagtctcc gatttccatc tcgatctctc agttccagaa ccgtggggat gtcgaaacaa
     3541 ggacacgcgg cgcagggcta tccgccagca ggtttgaccc ccgccgacct caattcaagc
     3601 tttcccctgc ggcgcctggc cggccgccgc gagcatcggg tcgtcgatgc gccggaagac
     3661 ccggcggagc acacgggact cgaaacgcca gccgtcggcg gtccgccgga agcggtcggc
     3721 gtactcgccg acggtggcgg gcagggccgc cggccgggtt ccggccgggt cggcgggcgc
     3781 ggcgctgttg aacacgatca ggtagcagac gcccgtcgcg ctgtcggggc cggttacctc
     3841 gatcaccggg ttggtgatca cgtggcgcat cgtcaggccg gtcgccgcca gcgcctggat
     3901 cgaggctcgg atggcgtcgt ggcccgtgaa cggcgggtgc tcgggaacgg tgatcgaggc
     3961 gtcggtcgtg aacagcccca cgaacccctc gacctcgccg cggtcggcga acacgccgta
     4021 ctgcatctgc agcttctggc aggcctgttc ggccagcatc cggtcgacgg gcgtcatacc
     4081 ggccttcctt tccctgctga cggcgcgggc agggcccgca gcacgtccgc cacgaaggcc
     4141 gtggcgtccc aggtgtcgaa ctcgctgagg ccgacgcgga ccaccaccag atcccgcgat
     4201 ggaatgatca ccacgtgctg cccctggtgg ccgttcatgt tgaacatgtc cggcgggatt
     4261 cccaggtcat gcgaatgctt cccccgcagc cagaactggg cgccgtaccg gccgtccgac
     4321 cccggcgtgg gcctgcgcac gtaatcaacc cagccctcgg gcaggatccg tttgccgtcc
     4381 cacagtccgt cgttgaggta gagcaggccc aggcgaccgt agtcccgggc gctggcgtag
     4441 aaactgctgc cgccgacgag gttgccggcc gcgtccggct cgaacacggc cgtgcgcatg
     4501 cccagcggct cgaacagcgc cttgcgcgga aacgcgaagg ccgtcggcag gtcggggccc
     4561 gccgcctcgg tgacgatccg gcccaggatg ttcgacgtgc cggtcgagta ctgccagcgg
     4621 gtcccgggcg gaacctcgag cggcttggcg gccgaatagc cggccagatc gccgccgacg
     4681 tattgcatgg tgatgtcgga cttcaccttg ctgtagctct cctcgaactg cagcccgctc
     4741 gacatgcgca tgaggttgtc gagggtgatc gccccgcgcg gatcacccgg gccgccccac
     4801 tcggcgatcg gggccggctt gttcaggtcc agcctgccct ggccgaccag gatgccgatc
     4861 agcgtcgccg tgacgctctt ggccaccgaa ttcgacagat gggccgtctc ggtgtcgaag
     4921 ccctcgccgt agcgctcgcc gatgatccgg cccttgtaga tgatcaccac gccgcgcgtg
     4981 ttgatcggcc gggcggggtc ggactcggtg aacaccttgt cgatcgccgc ggtcagcgcg
     5041 gagccgtcga cgccgggcgg caggtggccg atctgcgtcg cgccgccctc gggccagagg
     5101 gtgtccggat ccggccgcgg cagggcggtg gcgacgccct ggccctgggc gcgcaaagcc
     5161 gccacgctgg ccccctcggc cgccgtgcaa ccgatgccct gccggaagat cgcggcgcgg
     5221 gtcttgtagc cccagaggct ggcggtcacc gtcttgccgg ccagatccac cttcggcttg
     5281 accaccccaa gatattttcc cggggtcgac agccgcttga agtcctgccg ccaggccaac
     5341 gccgggtcgc gtccggcgac gaagatgtcg gtgcagagca tcttggccga gccgctggtg
     5401 aacaccgtga ccacgtcgcc aatggcgaca ttgaccgcca cggccccggc cagcagcacc
     5461 cctgcggtcg cggccccgac gacgtaccgg cgcttcatgt cagttcaccc gccggtcgcg
     5521 gccggcccag tagggctcgc gcagatcgcg cttgagcacc ttgcccgagg cgttgcgggg
     5581 cagggcgtcg atgaagtcca cggacttggg tgtcttgtat ccggcgatcc gctggcgggt
     5641 gaagtcgagg atgctggcgg cgtccgcggc gtggccgggc ttgaccacga ccacggcctt
     5701 gaccgcctca ccccatttcg cgtcgggcac cccgatcacg gccacctcgg cgatgtcggg
     5761 atggccgtac atcgcgttct cgacctcggc cggatagacg ttctcgccgc cggtgacgat
     5821 catgtccttc acgcggtcgc tgatgaagac gtagccgtcc tcgtccatca tccccgcgtc
     5881 gcccgtccgg acccagccgt cggcctggat cgtgcgggcg gtctcggcgt cctgcttcca
     5941 gtagcccacc atggttgtgg gggacttgat gcagatctcg ccgacacggt tgggtccgaa
     6001 gacctcgtcg ccggcggggt ccatgatccg gatctgcgcg ccgggaatca ccttgccggc
     6061 cgagcgcagg cgcggattgc ccgcccggtg gtcgtctcgc ggcagatagg tcaccgcgcc
     6121 ggtggtctcg gtcatgccgt aaagctgcac gaagccgcac ttgagcgtgt cgatggcccg
     6181 ctccagcagg tccagcggaa tggccgaggc cccgtagagg aaatcggtca tcgaggcgaa
     6241 gtcggccggg tcgagccccg gcgtgtcgag gatcatctgg atggtggtcg gcaccagcac
     6301 cgtcttggag atccggaagt cccggatgct gttgatcacc gcggtgttgt cgaactcgct
     6361 gagcacgatg ttggtggcgc cggaatagag tccgacgacg ccccagcccg tgccgctgat
     6421 gtggaagatc ggcatcgcga tcaggctgac gtcctccggc tcccactggt tccagtccat
     6481 gtcggacccg aacgcgcccg ggtgcgtcgc tgcgtattcg ttgaaggcgt agaagttgcg
     6541 gtgcgccagc tgcacgccct tgggccgccc cgtggtgccg ctggtgtaga gctgcaccgc
     6601 cacgtcgtcg cgcgacacgg gctcggcctg gtcggcgtcg ctctggcggt cgcgccaggt
     6661 ctggtaatcc tcccggcgcc cgcccgggtc catggtcacg atcttgcgca ccgtgggcaa
     6721 ctgaccctcg atgcgctcga ccaggggcag gcagtgaccg tcgacgaaca gcacctccgc
     6781 ctcggcgtcg ttggtgatgt agaccacctc cggttcggcc agccgccagt tgaccggggc
     6841 cagcactgcg ccgatgcgcg ccgcgccgaa caggatctcg aagtaccggt ccgagttctt
     6901 gcccaggtag gaaatgcgcg agccgcgccg gacgccctcg gccagcaggc cgttggccac
     6961 ccgggccgtg tgcttgtcga attcggcgaa ggtcgtcgcc cggccctcga accacagggc
     7021 cagcttgtcg ccgcgcgtcc ggccatggaa ccgcggcgcg tcgcccaggg tgcggatttc
     7081 cgggaaatag gcgatcggcg gcttcgccag cgtggcggcg ttggtcatgc actctcctcc
     7141 agggaccccg ttgcgagccc ggccaggtga tcctggtgcc gcgccctcag ggccttcttg
     7201 tcgatttttc cggtggcggt gagcggcagc tcgtccacca cgaccacgtc atccggcagc
     7261 tgccagcggg ccagcgtgcg tcgcatcgtc tcaagcacgg cgcccgggtc aagcctgacg
     7321 ccgggggagg ccgtgaccag catcagcggc cgctcctgcc agcgcgggtg cggcacggcg
     7381 atggccgcgg cctgggccac gccgggcgtg tcgcaggcgg ccttctccag atcggtggag
     7441 ctgatccatt cgccgcccga cttgatcagg tccttgaggc ggtcggtgat ggccacggcg
     7501 ccgtgctcgt cgactgtgcc gacatcgccc gtggggaacc agccgtcgtc ggtcaggacg
     7561 tcgccgccct ctccgcgata gtagccgctc gcgacgcagg ggccgcgcac ctgtatgtcg
     7621 ccggcgctga ccccatcgtg cggcaggacc gcgccgtcgg ggccgagcac acggatttcg
     7681 accggataga gcatgcggcc cgcgcggcca tgcagggccg cctcgtgccg ttcggcggga
     7741 agagcctcga tctctggcgt cgagccggtg aacgagccga gcgggctgag ttcggtcatg
     7801 ccccagccgg tgaccacggc gacgccgtat tgtccgcgca accgctggct catggccggt
     7861 ggcacggccg atccgccgat cagggcgcgc ttcagcggcc cgaggtcgcg accgctgcga
     7921 tccagttcgt cgaagacgcc ggtccagacc gtgggaacgc ccatggccag ggtcgcgccc
     7981 tcggcgtgca gcaggtcgac caccgtcctc gggtcgggcg cggcccccgg cagcaccagc
     8041 ttcgcgccgg cgatggcgca gctgaagggc agcccccagc cctgggcgtg gaacatcggc
     8101 accaccagca gcaccgaatc gaacggggtc aggccgaagg cgtttgccga ttgggaatag
     8161 agcgtgtgca gaacggtcga gcgatgggag ctcaggacgc ccttgggcat gccggtcgtg
     8221 cccgaggtgt agcagagcag ggccgccgtc cgttcgtcga actcgggcca gcggtagtcg
     8281 tcatcggcca gcaggtcttc gtaggcgatg accgggaagg ggagctcggc cggcagttcg
     8341 tcgcggccac ccagcagcac atagtgttcc accgacggca gcttgggcgc caggcgaacg
     8401 acaaggccca gcaagtcggg gtcgaagaac agcacccggt cctcggcatg gccggcgatg
     8461 tagaccagct gttcctccca gagcctcggg ttgagggtgt gcagcacggc gcccatgccg
     8521 ggtacgccgt agaacagctc gaaatgccgg tgggtgttcc aggccaggct ggccagacgg
     8581 tcgccgggcc ggagacccag ccgctccagt cccttggcca gccgccgcgc gcggacctcg
     8641 gcctgggcga aggtgaaccg ggcaaggctt ccgggaagcc gcgagacgat ctcgcgcgga
     8701 ccgttgaatg tcgccccgtg cgtcagcaac gaggcgatca gcagcggccg gtcctgcatc
     8761 agaccctgca acggcgtcgt catcggagga tttcgcgctc ctcgagattg gcctcgatct
     8821 cggcccgcgt gcgccagacc gggcgcaggg tcttggccga gtagagcctg agctgatcgc
     8881 cgtcgtgagg cgaccccggc tggctggcgt tgccgtagga cagcagcacc cgggccctga
     8941 ccggtctgga gaactccatg atgctgacga aactgtcgcc ctggaaggcg cggcgcttgc
     9001 cgtcggcgtc gggcaggtag tcggtcacac ggaacacgcc gtaaccaccg gtcgcaccgt
     9061 tgccgggcag gtcgatcccg ccgccccacc gcagccggta gacgtcgccg taggggacat
     9121 ccagcgcgcc gtatctggcg atcgtttcgt cggccgcggc ctccagcagg gcgacggcgc
     9181 gggttgggtc ggccaggccg gccggcgtgg ccattggccg ggccgcgacc caggtctcgc
     9241 ggaacagggc cggatccatg tcgtagggtc gcttgtactt gcccagccag gccatgaaga
     9301 ggatcgcgcc gcggctgtcg ccgtccatct cgcggtccca ggccgccagc acggcggcgg
     9361 ccttcaacgc cctctctccg cccccggtct tcgcggcggc gaccaggtcg tcgaccagcc
     9421 ggtcggccag ttcggaccgg gtggacagct tgtcctcgac cacatcgtcg aaggtcatca
     9481 tcggcctggc ggtgatcatg cgcagcgacc gctgcgtgcg cgggccgagg gtgcgcggcg
     9541 acatccaggc cgggaactgc tcgggcgtaa aggtcgccgg gtcggtgacg taccagggcg
     9601 gttcgttctc gttctgcagc cagccggcgg gcggattgaa ggtctggggg agagcctcga
     9661 acggcagggt gtcggtccac agcgtgcgcg aggtgtcgcc ggggacaatg ccgtcccaga
     9721 aggcgtagtc gccgtcctcg cgtgccggaa tccggccgga ggcgaggtag aagatgttcc
     9781 ccccggcgtc ggcgtagacc agattctgct tggggaactg catccgcgcg atggccttgc
     9841 ggaactgttt ccacgaccgc gagacggtca tgtcccagta ctgctggtac tggaacggca
     9901 tgtcgatcgc ggccagccgg acggccaggg ccttgcccgg cttctcgccg atcaccggac
     9961 cctggaccga ccagcggatg accagcgggc ggtcctcgag ggttccgtcc ggctggcgga
    10021 ccccgaagct ggtgcgggtt tcctcgaacg gccggacggc gccgtcgtag acatagccgc
    10081 cgtcggccag ggtcagttcg tagacgtcga catcgtccag cgtgttgacc gttccggtcc
    10141 agcccaggtg atcgttgaac cccccgctga tcacgggcgt gccgacctgc atcgcgccgt
    10201 agaagtccat gctgggggac ttggcgtcga cctcgaacac ccggaacagg ccgccgaagg
    10261 cgaagtcgcc ccacggaatg tgcgggttca tgaccagcat ggcgttgccg ctggccgatt
    10321 tcttcgggcc gatggcccag gcgttggagc cgcgcgacat ccagctgtcg acctcgccct
    10381 tcagggccgc gaaggcgaag ggcagcacgc cggagcgttg ctgatgggcc atgaagtcga
    10441 ccggatgcac gggcaggacc tgtcggacgt ccgcggcgat cttgtcggga tgggccgcgg
    10501 cgtaggcgtt caccccctcg gcgaaggcgt ccagcttggc gcggaaggcc ggcgtctgtt
    10561 gcgcgagggc ccgttcagcg cgttcgccga tcttcaatgt gcggacctgg atgtcggatt
    10621 cgacgtagga ctccccccag tactcggcgg cccgcccgcg cgcctggccg tagaggcgca
    10681 gcatgacgtt ggcgtggttg tgcatggtgg cccagccgag ggcgtagaat aggtcgttgt
    10741 ccgtccgcgc gaagatgtgc gggacacccc agctgtccca caggatttcg gcccgcgggg
    10801 gcgggttcgc ccgggcccag gcggcctggg gggcgccgcc aaggccgagc aacagcagca
    10861 tgaccagggc cgcgcccagg tgtcgcatcg tgcgtcgcat gcttccctcg tccctgcccg
    10921 gatcaggcgg cttccgcgcg catgggttcg gcccaccagg cggacagcac ctcggcgcag
    10981 acccgctttc ccttggccag atgcttctcg agggtgctga ccgaaaggcc cagcgtctct
    11041 gacatttcct tgtaggagag ctcctggacg gtgcggagtt cgatcacttc gcgacagcga
    11101 ttcggcaggc tctcgagcgc gcggccatag gccgcctcct gctcgccggc gatgaggatc
    11161 tcctcggccg aggcggcggc gccgcggccg cagaccacgt cctccagcat cggctcgctg
    11221 cgatcgatgg gattgctgcg ccgctggcgc atgcggttca gcgcgaggcg gtagcccgtc
    11281 ttgaacaaga ccgcacgggg atgattgaaa ctcgcgaagt caggcgcgcg gcacagacgc
    11341 aggaacgcct cctgggccac gtcgtcggcg tcggcctccg acttgagcag ccggttcaga
    11401 taggcccgca ggcgatcgat gttgtcgcga tagagcgcgg cgagggcgcg gtcgccggtc
    11461 cggtcgcatt gttgcggcgc aatcccgtcc atgacccctc cccacgcgat tgaggtggcg
    11521 tttcgacccg tgggtcgaaa gactgacctc gacgtggaat gatcatgtct ctagtcgaaa
    11581 gcttgtcaat cggtattatc gagtcgatag gggggcgacc tgtcgcgcgt cctgaccgga
    11641 gtgattgacg atgtcgaaag ccacaccccg ccgtgcgtgg ggcgcaggaa tccagaaccg
    11701 cgacgaacag ttcgagatga aacgccaggt ggtcctgcgg acggctgcgc ggacctacag
    11761 caagcggggt ttctacgaga cgaccctcac cgacatcgcg gacgagctga acatctcgaa
    11821 gccgacgctc tactattatt tcaagagcaa ggacgacatc ctcttcgagt gccaccgcat
    11881 cggcatcga
//
LOCUS       DAORNX010000035        11851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4085_length_11851_cov_6.733554, whole genome shotgun sequence.
ACCESSION   DAORNX010000035 DAORNX010000000
VERSION     DAORNX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_11851_cov_6.733554"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>252)
                     /locus_tag="PLO65_02705"
     CDS             complement(<1..>252)
                     /locus_tag="PLO65_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27192.1"
                     /translation="VLLSVSDSGVGMSPDVMARAFEPFFTTKKVGKGTGLGLAQVYGI
                     ARQAAGAARIESREGEGATVTIYLPRIEQAAAAEPGPRE"
     assembly_gap    253..364
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<365..795)
                     /locus_tag="PLO65_02710"
     CDS             complement(<365..795)
                     /locus_tag="PLO65_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRD27193.1"
                     /translation="MNLELEEQVHRRETAEEALRQAQKMETLGQLTGGIAHDFNNLLQ
                     SVQGSLDLIGRRPEDAARVRRLAAAGLEAAERGARLTAQLLAFSRAQKLEMRPLELAS
                     VARHLREILPSALGPGTRVVFDLEDAPMPVVADVTQLEVAVL"
     assembly_gap    815..939
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<940..1377)
                     /locus_tag="PLO65_02715"
     CDS             complement(<940..1377)
                     /locus_tag="PLO65_02715"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HRD27194.1"
                     /translation="MSWLGRSGHDWRAAATVTGVWVLVAASLVIGVANENAYWQRRGD
                     SLAAQAGILAGEVEPALVFQDAAAAREAVGALAADRQIEAAGIYDGRGRLIAQYVRDS
                     ARTPARAPPPGRGRDQGKSTVAGLVLHEGKPIGSVFLRTEAEPL"
     gene            complement(1374..1907)
                     /locus_tag="PLO65_02720"
     CDS             complement(1374..1907)
                     /locus_tag="PLO65_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HRD27195.1"
                     /translation="MFAPRVIGLVALSAALVLAPCAAVRAEPSLEYPVKAAFLYKFGS
                     YVDWPPSAFAGPREPVVVCVVGHDPFGVLLDRTVRNETINDRPVVVRRMRIAAPGAPC
                     HIAFLGGSREQPAEAAAAIFAGSPVLTVSDDAAGSVVRFRVVNNRVRFEIDQRAAEAD
                     GMTISSKLLNLAVAVIR"
     gene            complement(1907..3802)
                     /locus_tag="PLO65_02725"
     CDS             complement(1907..3802)
                     /locus_tag="PLO65_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27196.1"
                     /translation="MARALVCSAIMAVAALLASGARAAETTGDLARLSIEDLANVEIV
                     SVSKHPERLSEAAASVFVISGDDIRRSGAQSLPEALRLAPNLNVQRVDALDYGISARG
                     LNGFESANKLLVMIDGRSVYSPFFAGVEWSQLQPDLPDLDRIEVISGPGGALWGANAV
                     NGVINVVSRPADQTQGLLVEAIGGSGDDSATVRYGGALGPAGAFRVYVNGVNRADTLR
                     GGRDAGDDWDGGQFGFRTDLDGGRDRFTLQGDAYLDAVPSQIPSRPEGKLEGSNLLGR
                     WTRDMAGDSRLELQAYYDRYERRARGILDGVVTWDFQAQYSFTWGANRIVAGAGYRRW
                     NDRFANYVNGFVLDPPSRTTTLGNVFAQDQIDLGDVTVTLGLKAEDNSYSGVELMPSV
                     RLAWKTSETGMVWGAVSRAVRSPSRLDRELVFPPFLLASTFEPERLVAYETGYRGRPA
                     ARLSLSATVFYHRYEAIRSNEATPVTLFPIRIGNGLEGETWGVETWADVDLSPDWRLG
                     AGLTAMGKDFRVSAGSTDISHLAAAGHDPALQASLRSQTRLTPTLDLDVRLRYVGDTA
                     RERVGGYLNAPAYVEADARLAWQVSDRVELSVAGLNLLDDEHVEATEPRRTALRRSIQ
                     AGLRFSW"
     gene            4002..5183
                     /locus_tag="PLO65_02730"
     CDS             4002..5183
                     /locus_tag="PLO65_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD27197.1"
                     /translation="MPYVEGQTVHDADAHVMELPGTIERYADPAWRDALAARLNRPSR
                     DEAWVERAIALQRDPEFRAGQVENLLLRKNHQALGAFDREDRPLAVDLLGFASQLVFT
                     TTGLSLFQLEQEDDPALAVEAARAHNRMMVDFCAVDRRLLPTGYVPLVDRARAAEVAR
                     EAIGLGCKGLMIPSQVPRGHSPSHADFDALWATAQDAGLPILFHVGGEQKINPAYFNT
                     GGPQVLDFHGGAENFTSVSFMAIPVSIWQTLPVLVFDGVFDRFPDLKFGAIELGAGWL
                     PSLMQFMDSGAMAFGREERLQRLSAPPSDILRRQFFATPYPHENVGWIIDNVGEDMCL
                     FSSDFPHVEGGRNPLKRFNESLEGVSATGRRKFFRDNFIALMGQGLAAELHDLPGLAA
                     A"
     gene            5306..5632
                     /locus_tag="PLO65_02735"
     CDS             5306..5632
                     /locus_tag="PLO65_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27198.1"
                     /translation="MKTRLLIAIAGLALIATPALASSTTVEFAGSNGQTTTVVFNSDG
                     TASANGAAPAPYTVDQAAKKICGAVEGQELCVTFGEMGSTVGFSTTYTDTAGNSGTAT
                     ITAVSE"
     gene            complement(5694..5783)
                     /locus_tag="PLO65_02740"
     tRNA            complement(5694..5783)
                     /locus_tag="PLO65_02740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5747..5749),aa:Ser,seq:tga)
     gene            complement(5825..6226)
                     /locus_tag="PLO65_02745"
     CDS             complement(5825..6226)
                     /locus_tag="PLO65_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD27199.1"
                     /translation="MATIRIETDIAAPAGQVWDAIRDYGALHTRLVPGFVVDTRLEGD
                     SRLVTFGNGMVARERIVGLDDDARRFAYAVVSERLVHHSASNQVLPDGEGGCRFVWIA
                     DVLPDAAVDSIAPMMRQGAAVLKATAEAAVR"
     gene            6385..6975
                     /locus_tag="PLO65_02750"
     CDS             6385..6975
                     /locus_tag="PLO65_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRD27200.1"
                     /translation="MPTPDQPDAIALLKADHRKVEDLFASFEKARGADRKQALVQQIC
                     TELTVHTIIEEEIFYPACKGAVEEDLLDEAYVEHDGAKVIIAELMASAPDEAFYDAKV
                     KVLSEQIEHHVEEEEKRSEGMFSQARKAGLDMDMLGDQMRARKAQLMETFERDGLPPP
                     ETRSFTGHDLEEGEPVDDEADTSMSDRPGDSPRAGQ"
     gene            6972..7637
                     /locus_tag="PLO65_02755"
     CDS             6972..7637
                     /locus_tag="PLO65_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27201.1"
                     /translation="MIRDRLRDNGLTLALLALFAGSVIGQAWAGWSADNHDRTLHGLS
                     ALGLASYLTSGACLSALFENWESEFLQMSAYVVLTAYLVQRGSVESRDPDKPAPQDRD
                     PALDAGRPDAPGPVRAGRLARAAYSHSLGLALTALFAACFVLHWLNSAREANDDALIH
                     GEAPAGLVAHLGDARFWFESFQNWQSEFLSTAVLIVLAIVLRERQSPESKPVGAPHGQ
                     TGE"
     gene            7652..9874
                     /locus_tag="PLO65_02760"
     CDS             7652..9874
                     /locus_tag="PLO65_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27202.1"
                     /translation="MGEPATNAETGLKGLPVGALALEAELAGKGMQALAPIAFGGEQL
                     EPRIGRDGLWFFIGGRLALRTAWGPGGDLSARRLSDRCFEVESSLGRFVVDLALEPQD
                     RLLRVTTRFTPARDLLAPDWPRDLYVLNGAGRPCHLSSGAGGRVEAAQRGLNVGLVFF
                     TLRAPDFGTVMYLQNLTALNDWFRLTGTTPDGVVGGEWPELGYRPKAFTERALPAGET
                     LVLSDVLLTWTPRVAEDDAAEADHFLTLLAKTCRRLDDAPPTQFRDWPDRCARTLRHL
                     ARSPKATIRHYGHTYLHPYTAAEYPDCMVQLSVAQSLHEYAVWRGRPVPLEARLLAGT
                     NRFYDPQLGTLRRYLPNVGDDKNADEVDSWYLYHPLLNLGRLALAGDAACRRLFFDCI
                     GFAIRAARHFRYDWPIKFDVRTLEVITADRGQAGVGQSDVGGIYAYVMLLAHDLTGEA
                     RYREEAVAALRALKGWRFELLYQANLSAWGAAAAVRLWRDTGDERFLGTAKALLAGVF
                     HNSLMWESQIGAAVHYPNFLGATCLHDGPYMALYECFETFDAFDEILARGGEALPDAI
                     RLLLAEYRRHALNRAWFYFPDALPAELLPPEAEVRNGHIDRKLSFPVEDLYGDGQPPG
                     QVGQEIYGCGAPFTFATRAFHNLRGAPFLLFCEYRLADLVQAPGRVSFRLDAAPGYSG
                     TVRLLPRGASVAGVKVEGAVAARQEGGLTFTPDAGRTIAVTWEPSGRSRPSGRTGASA
                     "
     gene            9871..10053
                     /locus_tag="PLO65_02765"
     CDS             9871..10053
                     /locus_tag="PLO65_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27203.1"
                     /translation="MSKDKLQSPGREENAAQPPGRRDGLVSDRKVREIGKSAATRPGP
                     DDVDPDVIGESFKRRP"
     gene            complement(10061..11461)
                     /gene="lpdA"
                     /locus_tag="PLO65_02770"
     CDS             complement(10061..11461)
                     /gene="lpdA"
                     /locus_tag="PLO65_02770"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRD27204.1"
                     /translation="MDFDVIVIGAGPGGYVTAIRASQLGLKTAIVERENLGGICLNWG
                     CIPTKALLKSGEVYEQLSHMADYGLSVSGASFDFDKIIARSRGVAKKMEGGVAFLMKK
                     HKVEVLMGAATLGKGAPAPTVTVALNGGGSRTITAKHVILATGARARTIPAIGLESDG
                     ERVWTYRDALTAKACPKRLIVIGSGAIGIEFASFYRALGSEVTVIEALDRILPVEDEE
                     VSKAAHKAFEKRGMTFRVGAKVTKLAKSKTGVAVSLEAGGKAETLEADVAIVAVGITG
                     NVENMGLEALGVAVDRGHIVTDGHGRTNVAGLYAIGDIAGPPWLAHKASHEGVHCVEH
                     IAGLKPANLTAPIPGCTYSNPQIASVGLTEAAAKAAGHEPKVGRFPFRVNGKAVAAGE
                     IEGFVKTVFDGKTGALLGAHMIGHEVTEMIQGFAVAMTLEATEEDLMATVFAHPTMSE
                     AMHEAVLDAWDRVLHI"
     gene            complement(11472..>11851)
                     /locus_tag="PLO65_02775"
     CDS             complement(11472..>11851)
                     /locus_tag="PLO65_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRD27205.1"
                     /translation="LITPIIRAAETKGLAQIAAESKDLAARARDKKLRPEEFQGGTFS
                     VSNMGMMGIRSFASIINEPQACILSVGAGEQRPVVRNGALAIATLMTVTLTCDHRAVD
                     GAIGGRFLSVFKALIEDPLTMIV"
BASE COUNT         1740 a         4149 c         3980 g         1745 t
ORIGIN      
        1 gctcgcgcgg tccgggctcg gccgcggcgg cctgctcgat ccggggcagg tagatggtca
       61 ccgtcgcgcc ctcgccctcg cgactttcga tgcgcgccgc gccggccgcc tggcgggcga
      121 tgccatagac ctgggccagg cccagcccgg tccccttgcc gaccttcttg gtggtgaaga
      181 agggctcgaa ggcccgggcc atcacgtccg gcgacatgcc cacgccgctg tcggacaccg
      241 acagcaggac atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnagcacc gccacctcca gctgggtgac gtcagccacc accggcatgg gggcgtcttc
      421 caggtcgaac acgacgcgcg tgcccggccc caacgcgctc ggcaggatct cgcgcaggtg
      481 tctggcgacc gaggccagtt ccagcggtcg catctcgagc ttctgcgcgc gggaaaaggc
      541 cagcagctgg gccgtcaggc gcgcgccgcg ctcggccgcc tcaagtcccg ccgccgcgag
      601 ccgccggacg cgggcggcgt cctcgggccg gcgaccgatg aggtcgagac tgccctggac
      661 gctctggagc aggttgttga agtcatgggc tattccgccg gtcagctggc cgagcgtctc
      721 catcttctgg gcctgccgga gcgcctcttc ggccgtctcg cgacggtgaa cctgctcttc
      781 cagctcgagg ttcatgcgcc gcagcgattc cgccnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aggggttcgg cctcggtgcg
      961 caggaagacc gatccgatcg gcttgccttc gtgcagcacc agcccggcca ccgtcgactt
     1021 gccctggtca cgtccgcgcc cgggcggcgg agcccgcgcg ggcgtccggg cggagtcgcg
     1081 aacatactgg gcgatcagcc ggccgcgacc gtcgtagatt ccggcggcct cgatctgccg
     1141 gtccgcggcc agggccccca cggcctcacg ggccgccgcg gcgtcctgga acaccagcgc
     1201 cggctcgacc tcgccggcca ggatcccggc ctgggcggcc agggaatccc cgcgccgctg
     1261 ccagtaggcg ttctcgttgg ccacgccgat gacaaggctg gcggccacca gaacccagac
     1321 gccggtgacc gtggcggcgg ccctccagtc gtgtcccgag cgacccagcc agctcatcgg
     1381 atcaccgcca cggccaggtt gaggagcttg gagctgatgg tcatgccgtc ggcttcggcc
     1441 gcacgctggt cgatctcgaa ccggacgcgg ttgttgacga cccggaaccg gacaaccgaa
     1501 ccggccgcgt cgtccgacac ggtcagcacg ggtgagcccg cgaaaatcgc cgccgcggcc
     1561 tcggcgggct gttcgcgcga cccgcccagg aaggcgatgt ggcacggcgc gccgggcgcg
     1621 gcgatcctca tccggcgcac gacgaccggt cggtcgttga tcgtctcgtt gcgcaccgtg
     1681 cggtcgagca ggacgccgaa cggatcgtgg ccgacgacgc acacgaccac cggttcgcgg
     1741 ggtccggcga aggccgatgg cggccagtcc acataggagc cgaacttgta gaggaaggcc
     1801 gccttgaccg gatactcgag cgacggctcg gcgcggaccg cggcgcacgg agcgaggacc
     1861 agcgcggcgg acagggccac gagcccgatg acccgggggg cgaacatcac cagctgaaac
     1921 gcagcccggc ctggatcgac cgccggaggg ccgtgcgccg gggctcggtc gcttcgacat
     1981 gctcgtcgtc cagcaggttg agtccggcga ccgacagctc gacccggtca ctcacctgcc
     2041 aggccagacg ggcgtccgcc tcgacatagg ccggcgcatt gagatagccg ccgacccgtt
     2101 cgcgcgcggt gtcgccgacg tatcgcagcc gcacgtcgag atcgagcgtc ggggtcagcc
     2161 gcgtctgcga ccgcagggag gcctgcaagg cgggatcatg gcccgccgcc gccaggtgcg
     2221 agatgtcggt gctgccggcc gagacgcgga agtccttgcc catggcggtc aggccggcgc
     2281 ccaggcgcca gtccgggctg agatcgacat ccgcccaggt ctcgacgccc caggtctccc
     2341 cttccaggcc gttgccgatg cgaatgggaa acagggtcac cggcgtggcc tcgttcgaac
     2401 ggatcgcctc gtaccggtga tagaagacgg tcgccgaaag cgacagccgc gccgccggcc
     2461 ggccccggta gccggtttcg taggcgacca ggcgctccgg ctcgaaggtc gaggccagca
     2521 ggaagggcgg gaagaccagc tcgcgatcca gccgggaggg actgcggacc gcccgcgaca
     2581 cggcgcccca gaccatgccg gtctccgagg tcttccaggc cagacggacg ctcggcatca
     2641 gctcgacccc gctgtagctg ttgtcctccg ccttcagccc gagcgtcacg gtcacatcgc
     2701 cgagatcgat ctggtcctgc gcgaacacgt tgcccagcgt cgtggtccgc gatggcggat
     2761 cgagcacgaa gccgttgacg tagttggcga accggtcatt ccagcgacga tagccggccc
     2821 cggcgacaat ccggttcgcc ccccaggtga agctgtactg ggcctggaaa tcccaggtga
     2881 cgaccccgtc gaggatgccg cgggcgcgcc gctcgtagcg gtcgtaatag gcctgcagct
     2941 ccagccggga gtcgccggcc atgtcgcggg tccagcgccc cagcaggttg gagccctcaa
     3001 gcttgccctc cggccggctg gggatctgcg agggcacggc gtcgaggtag gcgtcgccct
     3061 gcagggtgaa gcggtcgcgt ccgccgtcca ggtcggtgcg gaagccgaac tggccgccat
     3121 cccagtcgtc gccggcgtcc cgcccgccgc gcagggtgtc ggcgcgattg acgccgttga
     3181 cgtagaccct gaaggccccg gccgggccga gcgcgccacc gtagcgcacc gtggcgctgt
     3241 cgtcccccga gccgccgatc gcctcgacca gcaggccctg ggtctggtcg gcgggacggc
     3301 tgaccacatt gatcacgcca ttgaccgcgt tggcgcccca gagcgcgccg cccggcccgc
     3361 tgatcacctc gatccggtcc aggtccggca ggtccggctg cagctggctc cactccaccc
     3421 cggcgaagaa cgggctgtag acgctgcgac catcgatcat caccaacagc ttgttggcgc
     3481 tctcgaagcc gttgaggccc cgcgccgaga tgccgtagtc gagggcgtcg acccgctgga
     3541 cattcaggtt gggagccagg cggagggcct ccggaaggct ctgggcgcca ctgcgcctga
     3601 tgtcgtcgcc gctgatcacg aacacggacg ccgccgcctc cgaaagccgt tcgggatgtt
     3661 tggagaccga cacgatctcg acgttggcca ggtcctcgat cgaaaggcgg gcaaggtcgc
     3721 cggtggtctc ggcggcgcgc gcgccgctcg ccagcagggc cgcgacggcc atgatggctg
     3781 aacagacaag cgctctggcc atgctcgtcc ccccctggcg cgaccgcgac cggcaacggc
     3841 ccgcgcagtc ggcgtcggcc gccggataca cctccgccat gccgctaaat gcgcgatggc
     3901 cgaaagggtt cccgccaggc gagccttgac gggcgggttc gatttccgcg caatggcgcc
     3961 atgcgggccg gcgtcaacga gacccgcgcg gaggatccga gatgccctac gtggaaggtc
     4021 agaccgtcca tgacgccgat gcgcatgtca tggagctgcc cggcacgatc gagcgctacg
     4081 ccgatccggc ctggcgcgac gccctggcgg cccggctgaa caggccgtcc cgcgacgagg
     4141 cctgggtcga gcgggccatc gccctgcagc gcgatcccga gttccgcgcc ggccaggtcg
     4201 agaacctgct gctgcgcaag aaccatcagg cgctgggcgc cttcgaccgc gaggaccggc
     4261 ccctggcggt ggacctgctg ggcttcgcca gccagctggt gttcacgacc accggcctca
     4321 gcctgttcca gctcgagcag gaggacgatc cggccctggc cgtcgaggcc gcccgcgccc
     4381 acaaccgcat gatggtcgac ttctgcgccg tcgaccggcg cctgctgccg accggctatg
     4441 tgccgctggt cgaccgggcc cgcgccgccg aggtggcgcg cgaggccatc ggcctgggct
     4501 gcaaggggct gatgatcccc tcgcaggtcc cgcgcggcca ttcgcccagc catgccgact
     4561 tcgacgcgct ctgggccacc gcgcaggacg ccggcctgcc gatcctgttc cacgtcggcg
     4621 gcgagcagaa gatcaacccg gcctatttca acaccggcgg tccccaggtg ctcgacttcc
     4681 acggcggggc cgagaacttc acctcggtca gcttcatggc cattccggtg tcgatctggc
     4741 agacccttcc ggtgctggtg ttcgacgggg tgttcgaccg tttcccggac ctgaagttcg
     4801 gggccatcga actgggcgcc ggctggctgc ccagcctcat gcagttcatg gactccggag
     4861 ccatggcctt cggcagggag gaacggctgc agcggctgtc ggccccgccc agcgacatcc
     4921 tgcgccgcca gttcttcgcc accccctatc cgcacgagaa cgtcggctgg atcatcgaca
     4981 acgtcggcga ggacatgtgc ctgttctcgt cggacttccc gcatgtcgag ggcggccgca
     5041 atccgctcaa gcggttcaat gaatcgctgg agggcgtatc ggcgaccggc cggcgcaagt
     5101 tcttccgcga caacttcatc gccctgatgg gccagggcct ggccgccgag ctgcacgacc
     5161 tcccgggcct cgccgcggcc tgatccggct acccgaatcg gggatggttc gctcgttaac
     5221 cttgattgcc gtcctcgcca cgggcgccta acctcccgcc cgacggcgcg ttcgacgccg
     5281 cgcgaaaccc aaggggggac tatccatgaa gaccagactg ctcatcgcga tcgccggcct
     5341 cgccctgatc gccacgcccg ccctcgcctc ctcgaccacg gtcgagttcg ccggcagcaa
     5401 cggccagacc accacggtgg tgttcaattc cgacggcacg gccagcgcca acggcgcggc
     5461 gccggcgccc tacaccgtcg accaggcggc caagaagatc tgcggcgccg tcgagggcca
     5521 ggagctgtgc gtgaccttcg gcgagatggg ctcgacggtc ggattctcga cgacctacac
     5581 cgacacggcc ggcaactccg gcaccgcgac catcaccgct gtcagcgagt aggcccccgg
     5641 gggcgcggcg gtcatcggcc gccgcgcccg tcctcgaaac ggcccgggaa ggatggcgga
     5701 cggggtggga ttcgaaccca cggcaggctt ccacctgcgg cggttttcaa gaccgctgcc
     5761 ttaaaccact cggccacccg tccgtggcgg cgcttctaac agcgcccggg cggggcctcc
     5821 agcgctatcg gacggcggcc tcggcggtcg ccttcaggac ggcggcgccc tggcgcatca
     5881 tcggcgcgat ggagtcgacc gcggcgtcgg gcagcacatc ggcgatccag acgaagcggc
     5941 agccgccctc cccgtccggc aggacctggt tggaggcgct gtggtggacc agccgctcgc
     6001 tgaccacggc gtaggcgaag cgccgcgcgt cgtcgtcgag gccgacgatc cgctcccgcg
     6061 cgaccatgcc gttgccgaag gtgaccaggc ggctgtcgcc ctccagccgg gtgtcgacga
     6121 cgaaaccggg cacgaggcgc gtatgcagcg cgccgtagtc gcgaatggcg tcccagacct
     6181 gtccggccgg ggcggcgatg tcggtttcga tgcggatcgt ggccatgacg gctcctctgt
     6241 ctgctgtccg accgacagtc tgccccggcc ccacccgcga cgctggcgac ttccggacat
     6301 cgtcaccggc gggcggaacc acaggccgcg atccctgttg agcaggagcg ccagggaggc
     6361 gcggccacca gaggacatcc ccccatgccg accccggacc aacccgacgc catcgccctg
     6421 ctcaaggccg atcaccgcaa ggtcgaggac ctgttcgcca gtttcgagaa ggcccgcggc
     6481 gccgaccgca agcaggcgct ggtccagcag atctgcaccg agctgaccgt gcacaccatc
     6541 atcgaggagg agatcttcta cccggcctgc aagggcgcgg tggaggagga cctgctcgat
     6601 gaggcctatg tcgagcacga cggcgccaag gtgatcatcg ccgagctgat ggccagcgcg
     6661 cccgacgagg cgttctacga cgccaaggtc aaggtcctca gcgagcagat cgaacaccac
     6721 gtcgaggaag aggaaaagcg ctccgagggg atgttctccc aggcgcgcaa ggccggcctc
     6781 gacatggaca tgctcggcga ccagatgcgc gcccgcaagg cgcagctgat ggagaccttc
     6841 gaacgcgacg gcctgccgcc gcccgagacg cgcagcttca ccggccatga tctcgaggaa
     6901 ggagagcccg tcgacgacga ggccgacacg tcgatgtccg accggccggg cgactcgccg
     6961 cgcgccggcc agtgatccgc gaccgccttc gcgacaacgg cctgaccctg gcgctgctgg
     7021 ccctgttcgc cggcagcgtc atcggccagg cctgggcagg ctggagcgcg gacaatcacg
     7081 accggaccct gcacgggctc tccgcgctcg gcctcgccag ctatctgacg agcggcgcat
     7141 gcctgtcggc gctgttcgag aactgggaga gcgagttcct gcagatgtcg gcctacgtcg
     7201 tgctcaccgc ctatctggtc cagcggggtt ccgtggaatc gcgcgacccc gacaagcctg
     7261 cgccacagga ccgcgacccg gcgctcgacg cgggccggcc ggacgcgcca ggccccgtgc
     7321 gggccggtcg cctcgcccgg gcggcgtaca gtcattcgct cggcctggcc ctgaccgcgc
     7381 tgttcgcggc gtgcttcgtc ctgcactggc tgaacagcgc gcgcgaagcc aacgacgacg
     7441 ccctcatcca cggcgaggcg ccggcgggtc tggtcgccca tctgggcgac gcccggttct
     7501 ggttcgaaag cttccagaac tggcagagcg aattcctgtc caccgcggtg ctgatcgtcc
     7561 ttgccatcgt cctgcgggaa cgacaatcgc ccgagagcaa gccggtcggc gctccccacg
     7621 gtcagaccgg cgaataggga ggcccccggt catgggcgag cccgcgacca atgcggagac
     7681 ggggctgaag gggcttccgg tcggggccct ggcgctcgag gccgaactgg ccggcaaggg
     7741 catgcaggcg ctggcgccga tcgccttcgg cggcgagcag cttgagccca ggatcggccg
     7801 cgacggcctg tggttcttca ttggcgggcg gctggccctg cgcacagcct gggggcccgg
     7861 cggggacctg tccgcgcgcc gactgtccga ccgctgcttc gaggtcgaat cctcgttggg
     7921 tcgcttcgtc gtggacctgg cgctcgaacc gcaggaccgg ctgctgcggg tgacgacgcg
     7981 gttcacgccc gcgagggacc tgctggcgcc cgactggccg cgcgatctct atgtgttgaa
     8041 cggcgccggc cgaccctgcc acctgtcgtc gggcgccggc ggacgcgtcg aggcggcgca
     8101 gcgcggcctc aacgtcggac tggtcttctt caccctgcgg gcgccggatt tcggcacggt
     8161 gatgtatctg cagaacctga cggcgttgaa tgactggttc cgcctgaccg gaacgactcc
     8221 cgacggcgtg gtcggcggcg aatggccgga gctgggctac cggccgaagg ccttcaccga
     8281 aagggccctg ccggccggcg agaccctggt cctgtcggac gtcctgctga cctggacgcc
     8341 gcgagtggct gaggatgacg cggccgaggc cgaccatttc ctgaccttgc tggcgaaaac
     8401 ctgccgccgg ctggacgacg ccccgccaac gcagttccgc gactggccgg accgctgcgc
     8461 ccgcaccctg cggcaccttg cgcgctcgcc caaggcgacc attcgccact acggccatac
     8521 ctacctgcat ccctacacgg ccgcggaata tcccgactgc atggtgcagc tgagcgtcgc
     8581 ccaatcgctg catgaatacg cggtctggcg cggccgcccg gttccgctgg aggcgcgcct
     8641 gctggccggc acgaaccggt tctatgaccc gcaactgggg actttgcgcc gctacctgcc
     8701 caacgtcggc gacgacaaga acgccgacga ggtcgacagc tggtacctct accatccgct
     8761 gctgaacctc ggccggctgg cgctggcggg cgatgcagcc tgcaggcggc tgttcttcga
     8821 ctgcatcggg ttcgcgatcc gcgcggcccg gcatttccgc tacgactggc cgatcaagtt
     8881 cgacgtgcgg actctggagg tgatcacggc cgaccgcggc caggcggggg tgggccagtc
     8941 ggacgtcggc ggcatctacg cctatgtcat gctgctggcc cacgacctga ccggcgaggc
     9001 gcgctatcgc gaggaggcgg tggccgccct gcgcgcgctg aagggctggc gcttcgagct
     9061 gctctaccag gccaatctca gcgcctgggg cgcggcggcg gccgtccgcc tgtggcggga
     9121 taccggcgac gagcgcttcc tcggcacggc gaaggccctg ctggcgggcg tgttccacaa
     9181 cagcctgatg tgggagtcac agatcggggc cgcggtccac tatcccaact tcctcggggc
     9241 cacctgcctg cacgacgggc cgtatatggc tctctacgag tgcttcgaga ccttcgacgc
     9301 gttcgacgaa atcctcgccc gcggcggcga ggccctgcct gatgcgatcc ggctgctgct
     9361 ggccgagtat cgccggcacg cgctgaaccg ggcctggttc tatttccccg acgccctgcc
     9421 cgcggaactg ctgccgcccg aggccgaggt gcgcaatggc catatcgacc gcaagctgtc
     9481 gttcccggtc gaggacctct acggcgatgg ccagccgccc ggccaggtcg gccaggagat
     9541 ctatggctgc ggcgcgccgt tcaccttcgc cacccgcgcc ttccacaatc tccggggcgc
     9601 gccgttcctg ctgttctgcg aataccgcct ggccgatctc gtccaggcgc ccggccgggt
     9661 gtcgttcagg ctcgacgccg cacccggcta cagtggcacg gtccggttgt tgccacgggg
     9721 cgcgtctgtg gccggcgtga aggtcgaggg cgccgtcgct gctcggcagg aaggtggcct
     9781 gaccttcacg ccggatgccg gtcgcacgat cgccgtcacc tgggaaccgt cggggcgttc
     9841 gcgtccttcc ggaaggacag gagcgtcagc atgagcaagg acaaactcca gagcccgggc
     9901 cgcgaggaga acgccgcgca accgcctggc cgccgcgacg gcctggtgtc ggaccggaaa
     9961 gtccgcgaga tcggcaagtc cgccgccacg cgtcccggtc ccgacgacgt cgacccagac
    10021 gtcatcggcg agagcttcaa gcgcaggccc tgagcctcct tcagatgtgc agcacccggt
    10081 cccaggcgtc gagcacggct tcgtgcatcg cctcgctcat cgtcggatgg gcgaagacgg
    10141 tggccatcag gtcctcctcg gtggcctcca gcgtcatggc cacggcgaag ccctggatca
    10201 tctcggtcac ctcgtggccg atcatgtggg cgccgagcag cgcaccggtc ttgccgtcga
    10261 acaccgtctt gacgaagccc tcgatctcgc cggccgcgac ggccttgcca ttcacgcgga
    10321 acgggaagcg gccgaccttc ggctcatggc cggccgcctt cgcagcggct tcggtcaggc
    10381 cgaccgaggc gatctgcggg ttcgagtagg tgcagccggg gatcggcgcg gtcagattgg
    10441 ccggcttcag gcccgcgata tgctcgacac agtggacgcc ctcgtggctg gccttgtgcg
    10501 ccagccaggg cggaccggcg atgtcgccga tggcgtagag gccagcgacg ttggtcctgc
    10561 cgtggccgtc ggtgacaatg tggccgcggt cgaccgccac gccgagcgct tcgaggccca
    10621 tgttctcgac attgccggtg atgccgacgg cgacgatggc cacatcggcc tccagcgtct
    10681 cggccttgcc cccggcttcg agcgacacgg cgacgcccgt cttgctcttc gccagcttcg
    10741 tcaccttggc gccgacgcgg aaggtcatgc cgcgcttttc gaacgccttg tgcgcggcct
    10801 tggagacctc ctcgtcctcg accggcagga tgcggtcgag ggcctcgatc accgtaactt
    10861 ccgatcccaa cgccctgtaa aagctcgcga attctatacc gatggcgcca gagccgatga
    10921 caatcagccg cttcgggcag gccttggccg tcaaggcgtc gcgataggtc cagacgcgct
    10981 cgccgtcgct ctcgagcccg atggcgggga tggtccgggc gcgggctccg gtggcgagga
    11041 tcacatgctt cgccgtgatc gtgcggctgc cgccgccgtt cagggccacg gtcacggtcg
    11101 gcgccggcgc gcccttcccg agcgtggccg cgcccatcag cacctcgacc ttgtgcttct
    11161 tcatcaggaa ggcgacgccg ccttccatct tcttggccac gccccgtgag cgggcgatga
    11221 tcttgtcgaa gtcgaagctc gcgccgctga ccgacaggcc atagtcggcc atgtgcgaca
    11281 gctgctcgta gacctcgccg ctcttgagca gggccttggt cgggatgcag ccccagttga
    11341 ggcagatgcc cccgaggttc tcgcgctcga cgatggcggt cttcaggccc agctgactgg
    11401 cccggatggc tgtcacataa ccgccggggc cggcgccgat gacgatgacg tcgaaatcca
    11461 tcacttctgc ctcaaacgat catcgtcagg ggatcttcga tcagcgcctt gaacacgctg
    11521 aggaaccggc cgccgatggc gccgtcgacc gcgcggtggt cgcaggtcag ggtcacggtc
    11581 atcaaggtgg cgatggccag ggcgccgttc ctgaccaccg gccgctgttc gcccgcgccg
    11641 acgctgagga tgcaggcctg cggctcgttg atgatcgagg cgaacgagcg gatgcccatc
    11701 atgcccatgt tggagaccga gaaggtgccg ccctggaatt cctcgggcct gagcttcttg
    11761 tcacgggcgc gggcggccag gtccttcgac tcggcggcga tctgggccag gcccttggtc
    11821 tcggcggcgc ggatgatcgg ggtgatcagg c
//
LOCUS       DAORNX010000036        11850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4086_length_11850_cov_7.222382, whole genome shotgun sequence.
ACCESSION   DAORNX010000036 DAORNX010000000
VERSION     DAORNX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_11850_cov_7.222382"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1900
                     /gene="phaC"
                     /locus_tag="PLO65_02780"
     CDS             101..1900
                     /gene="phaC"
                     /locus_tag="PLO65_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRD27206.1"
                     /translation="MDPAAFLSSEQREALEKLSANLARAAVTAQGAIAEAALRQADSP
                     AALTPDPFHVGPALTDVMGRLAAQPDRLMRAQADLFSRYLDLWQTTARRMSGEQVEPT
                     IVPPKGDKRFNDPEWGAHPVYDVMKQSYLLTSGWLHDLVAEVEGVDPLEKRRVEFFIK
                     MLTDAISPSNFLMSNPAALKEAMASGGESLVRGMENFAADLERGGGQLAISQTDMEQF
                     VVGENVATAPGKVVYQNDILQLLQYQPTTDQVREIPLLLFTPWINKFYITDLRPDNSL
                     IRWLTGQGFTVFVTSWVNPDETLAAKTFEDYLVEGIYDATAQVMKQCGTDRVNTVGYC
                     IGGTLLSCALAHMAAKGDKRINSATFFAAQQDFEEAGDLRLFVDDAWLRSIEETMDQS
                     GGFLPGKSMADTFNSLRGNDLIWSFFVNNYLMGKEPKPFDLLFWNSDQTRMPKALHLF
                     YLRNFYKDNALSKGKLTLAGVTLDLKKVQTPIYVQSSRDDHIAPFRSIYRGARLFGGP
                     VTFTMAGSGHIAGVINHPDAKKYQHWTNPALPPTVEEWQAGAVEHPGSWWPHWAAWLN
                     ERSGGMVAARDPSNGPLGKPLEDAPGSFVKVRS"
     gene            complement(2054..2506)
                     /locus_tag="PLO65_02785"
     CDS             complement(2054..2506)
                     /locus_tag="PLO65_02785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27207.1"
                     /translation="MIIRRAVPADTPTLRAMMASSNGYQDPAARSMIFGFAAGWSVPD
                     AGHEIWLAQDIHAVAGFYALIPLGKDQELDLFFTANDAQGIGVGRRLFAHMAERAAAL
                     DAKRVVISSNPEAAAFYRRMGAVDIGVSPPGDGISWERPKLELVLPGA"
     gene            complement(2503..3453)
                     /gene="argC"
                     /locus_tag="PLO65_02790"
     CDS             complement(2503..3453)
                     /gene="argC"
                     /locus_tag="PLO65_02790"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRD27208.1"
                     /translation="MSTPKVFIDGEAGTTGLQIRERLAGRTDLELLSIAPERRKDTEA
                     RAELLNAADLVILCLPDDAAREAVSLISNPAVKVIDASTAHRTAEGWTFGFPELCKSQ
                     RAAIKSAKRVSNPGCYSTGAIALIRPLVQAGLMPADHPVSVNAVSGYTGGGKPMIAEF
                     EDEAGPTFTKVPYRIYALGLEHKHVPEMQIRSGLLTRPLFTPAVGRYAQGMLVEVPLH
                     LAQMGNGPDLRDIHDVLTRHYKGESFVEVVSLKEIETLKTLDPEGLNGTNRMKLFVFG
                     SDEGRQARLVALLDNLGKGASGAAVQNLNLMLGLPESAGL"
     gene            complement(3661..4131)
                     /gene="rpsI"
                     /locus_tag="PLO65_02795"
     CDS             complement(3661..4131)
                     /gene="rpsI"
                     /locus_tag="PLO65_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRD27209.1"
                     /translation="MAEAQGFDALQSLSSQPEAAPAVQKLDKFGRAYATGKRKNAIAR
                     VWIKPGKGSITINGRDQEVYFARPVLRMMLAQPFQVTDRVGQFDVIVTVEGSGLSGQA
                     GAVRHGISKALTYYEPALRTALKPHGFLTRDSRVVERKKYGKAKARRSFQFSKR"
     gene            complement(4135..4608)
                     /gene="rplM"
                     /locus_tag="PLO65_02800"
     CDS             complement(4135..4608)
                     /gene="rplM"
                     /locus_tag="PLO65_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRD27210.1"
                     /translation="MMKTTASLKPAEVEKKWIVIDAENVVVGRLASFIAMRLRGKHRP
                     DYTPNVDCGDYVVVVNADKVKFTGRKLEQKIYYRHTGHPGGIKEITAGKQLAGRFPER
                     VLEKAVERMLPKESPLARKQLTHLRIFNGAEHTHQAQSPEPIAFAELNAKNVRSQ"
     gene            4835..6235
                     /locus_tag="PLO65_02805"
     CDS             4835..6235
                     /locus_tag="PLO65_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HRD27211.1"
                     /translation="MTLSRRNLLSAGAVSLAFSGLARAQEAPETYRNEITGYGPLRAD
                     PNGVFDLPEGFSYRVVSFAGQSMNDGYVVPANADGMACFPGRGSKVILMRNHENGLKH
                     RNLGPLGIGRSLRKRPKPDKVFDVDATGEPLSGGVSRLVYDTAAGRLVRAEMALFGTS
                     TNCSGGPTPWGSWLTCEETTTGPAEGLGKPHGWVFEVPARGRGLVDPVPLKAMGRFFH
                     EAAAVDPVTGIVYLTEDAPAPDRAGLLYRYLPDSPGHLAVGGRLQALAFRDDPGADAR
                     NWDGRVIWKQGDVRQAAWIDLTDVESPGDDLRHRGHAAGAVILGRGEGLFRGRDGFYM
                     SCTNSGPAKHGQILRYRPGPHEGQPGEADAPGTIELFIESTDDRVLDYPDNLTVAPNG
                     HLLVCEDRYSATLRNHLRAITPDGRSYTLGRNVYRDNAELAGVCFSPDGQTLFVNIYW
                     PGITLAVTGPWAALRP"
     gene            complement(6232..7263)
                     /locus_tag="PLO65_02810"
     CDS             complement(6232..7263)
                     /locus_tag="PLO65_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRD27212.1"
                     /translation="MTSFLRSDRSRPVAVWLLAVALLVLAMVVVGGATRLTGSGLSIT
                     EWKPVTGALPPMGEQAWQGEFAKYREIPQYTQLNRGMSLAEFKAIYWWEWSHRLLGRL
                     VGAAFAIPFVIFLIRKDIPRRLIWRCGAMLGLGGLQGAVGWWMVASGLAVRVSVAPER
                     LATHLGLALALFVLLVWTALDAEAGQPRQANPSRWRAGSLILLAAVFLQCLLGALVAG
                     ADAGFIDNDWPLMGGRLFPRDYAGDSLWATLAHNQASVQLHHRLGAYLLLAGGLTVAA
                     LAWRDRFLSPGARGLGLAVGGVVLLQALLGIATLMAGVPLWLGILHQLGAVALLATAT
                     AFAWRVRRL"
     gene            7374..7622
                     /locus_tag="PLO65_02815"
     CDS             7374..7622
                     /locus_tag="PLO65_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27213.1"
                     /translation="MGSPGLFIVVVIGLLAGAIGRALLGGRPSRFAGFSAGVAGAIVG
                     VPAAGALGWPVTTLWALALAALGGAVVFLAAARLLDRR"
     gene            7686..7934
                     /locus_tag="PLO65_02820"
     CDS             7686..7934
                     /locus_tag="PLO65_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRD27214.1"
                     /translation="MHGLNWVVIIIAGIFAGWIAERLMNRNHGILTNLIVGLVGAFIG
                     SWLAGAFGFAYSGLLPSILVAVLGAVVLLFVLGLFKRR"
     gene            7984..8193
                     /locus_tag="PLO65_02825"
     CDS             7984..8193
                     /locus_tag="PLO65_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HRD27215.1"
                     /translation="MPPRIRKLVGLLAMLVFLVAWAAGAVAIADRLPAHPAVSLLFFL
                     VVGTGWGLPLFPLLSWMNREPKPKG"
     gene            complement(8218..8294)
                     /locus_tag="PLO65_02830"
     tRNA            complement(8218..8294)
                     /locus_tag="PLO65_02830"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8258..8260),aa:Pro,seq:ggg)
     gene            complement(8375..8797)
                     /locus_tag="PLO65_02835"
     CDS             complement(8375..8797)
                     /locus_tag="PLO65_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD27216.1"
                     /translation="MAKGPHAFRTISEAAEELGVPQHVLRFWETKFSFIRPMKRAGGR
                     RFYRPQDIAVLRGVRALLHDEGYTIKGVQKLHKEQGVRRLSAAGEAAGTAMPADQASL
                     PEPREPVDLAGEARDRLTFALADLEAVKARLDGLLKGR"
     gene            complement(8806..9108)
                     /locus_tag="PLO65_02840"
     CDS             complement(8806..9108)
                     /locus_tag="PLO65_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRD27217.1"
                     /translation="MKGETLTRAELTEAVHEEVGLTRQDCGGLVERTLDLVAEALERG
                     ETVKLSGFGVFQVRAKRARMGRNPKTGEPAEIEPRRVIGFRASQVMKARIDRALSD"
     gene            complement(9199..10176)
                     /locus_tag="PLO65_02845"
     CDS             complement(9199..10176)
                     /locus_tag="PLO65_02845"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRD27218.1"
                     /translation="MSVVRSVVTGVGGYLPETVVSNDDLSKIVDTNDAWIQERTGIRR
                     RHRASEDQAVSDLAVEAARKALAAAGKTAADVDLIVLATTTADLTFPATAAIVQRKLG
                     CPVGIAFDVQAVCSGFVYALTVADGFVGRGHAKCALVIGAETMTRLMDWTDRGTCVLF
                     GDGAGAVVLEPGEGQGTTADRGLLGFALRCDGTKQELLYVDGGPSTTGTVGKLRMLGN
                     QVFKHAVINISEAVTAAAAQAGVQVSDIDWFIPHQANKRILQGVAHRLGIDEHKVIQT
                     VEDHANTSAASIPLAWAEGMADGRIRKGDLLLIEAMGGGLTWGACVIRL"
     gene            complement(10173..11237)
                     /gene="plsX"
                     /locus_tag="PLO65_02850"
     CDS             complement(10173..11237)
                     /gene="plsX"
                     /locus_tag="PLO65_02850"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRD27219.1"
                     /translation="MPQNIVISIDAMGGDHGPSVVIPGVDIAARAMPELRFLLHGDEA
                     RINAELARTPAARAASTVRHTDRAISMDEKPAQAMRRGKGSSVWNAVESVKAGEALAA
                     VSAGNTGALMAISKLVLRMAAGIARPAIVASWPTLNGVTAVLDVGANVECDAEQLVEF
                     AIMGAAFHHAVHGSARPTVGLLNVGSEEEKGHEEVREAHALLRATRFDLDYRGFVEGN
                     DIAKGTVDVIVTDGFTGNIALKTAEGLARFFASELRATFTSGPMAMLGALFASGALKK
                     MRQRMDPGAVNGGPLLGLNGIVVKSHGGADARGYAAAIRVAADLARSDFAGEIDRNMQ
                     RLTATLSESTATASPEGVTK"
     gene            complement(11332..11838)
                     /locus_tag="PLO65_02855"
     CDS             complement(11332..11838)
                     /locus_tag="PLO65_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRD27220.1"
                     /translation="MIRFGEAARGPVTRHLEADAATRTKVARHLGLVSIGSLVADVTV
                     RPWMDGVEIEGAFSADVTQTCSVTAEDFEETAEGEFLVRALPSGSPNAPQEDAEEIDL
                     DPDADDPPDVLEGEDIDLGGYVVEHLSLALDPFPRKPGAEFIAPKDEADLSPFAVLRL
                     LKKDDAPE"
BASE COUNT         1826 a         4161 c         4004 g         1859 t
ORIGIN      
        1 gggagcggcg aagaagcccg ccgccaggaa gccggccgcc gcccgcgcgt ccgccggcga
       61 atctcccgtc ccgccgccgg cgcctgaagc ggccgcggcg atcgatccgg ccgccttcct
      121 ctcgtccgaa cagcgcgagg cgctcgagaa gctgtcggcc aacctcgccc gcgccgccgt
      181 cacggcccag ggggccattg ccgaggccgc cctgcgtcag gccgacagcc cggccgccct
      241 cacccccgac ccgttccatg tcggaccggc cctgaccgac gtcatgggca ggctggctgc
      301 ccagccggac cggctgatgc gggcccaggc cgacctgttc tcgcgctatc tcgacctgtg
      361 gcagacgacg gcccggcgta tgagcggcga gcaggtcgag ccgaccatcg tcccgcccaa
      421 gggcgacaag cggttcaatg atcccgaatg gggcgcccat ccggtctatg acgtgatgaa
      481 gcagtcctac ctgctgacca gcggctggtt gcatgacctg gtcgcggagg tcgagggggt
      541 cgacccgctg gaaaagcgtc gcgtcgaatt cttcatcaag atgctgaccg acgccatctc
      601 gccgtcgaac ttcctgatgt cgaacccggc cgcgctcaag gaggccatgg ccagcggcgg
      661 cgagagcctt gtgcggggca tggagaattt cgccgccgac ctcgagcgcg gcggcggtca
      721 gctggccatc agccagaccg acatggagca gttcgtggtc ggcgagaacg tcgccaccgc
      781 ccccggcaag gtggtctatc agaacgatat cctgcagctg ctgcagtacc agccgaccac
      841 cgaccaggtg cgcgagattc cgctgctgct gttcacgccc tggatcaaca agttctacat
      901 caccgacctt cggccggata acagcctgat ccgctggctg accggccagg gcttcacggt
      961 gttcgtcacc agctgggtca acccggacga gaccctcgcg gccaagacct tcgaggacta
     1021 cctggtcgag ggcatctacg acgccacggc ccaggtgatg aaacagtgcg ggaccgaccg
     1081 ggtgaacacg gtcggctact gcatcggcgg gaccctgctc tcctgcgccc tcgcccatat
     1141 ggcggccaag ggcgacaagc ggatcaactc ggcgacgttc ttcgcggccc agcaggactt
     1201 cgaggaggcc ggcgacctga ggctgttcgt cgacgacgcc tggctgcgct cgatcgagga
     1261 gaccatggac cagtcgggcg gcttcctgcc cggcaagtcg atggccgaca ccttcaactc
     1321 gctgcgcggc aacgacctga tctggtcgtt cttcgtcaac aactacctga tgggcaagga
     1381 gcccaagccg ttcgacctgc tgttctggaa cagcgaccag acgcgcatgc ccaaggcgct
     1441 gcacctgttc tatctgcgca atttctacaa ggacaacgca ctgtcgaagg gcaagttgac
     1501 cctggcgggc gtgacgctcg atctgaagaa ggtccagacc ccgatctacg tccagtcgtc
     1561 gcgcgacgac cacatcgcgc cgttccgcag catctatcgc ggggccaggc tgttcggcgg
     1621 gccggtgacc ttcaccatgg ccggcagcgg ccatatcgcc ggggtgatca accacccgga
     1681 cgcgaagaag taccagcact ggaccaaccc cgccctgccc cccacggtcg aggaatggca
     1741 ggctggcgcc gtcgagcatc cgggcagctg gtggccgcac tgggccgcct ggctgaatga
     1801 gcgctcgggc gggatggtgg ccgcgcgtga cccgtcgaac ggccccctcg gcaagccgct
     1861 cgaggacgcg ccggggagct ttgtgaaggt gcggagctag ccccttcctc cccagcaagc
     1921 tggggagggg gaccacgcgg agcgtggtgg aggggcggcg cggacgccag cgaccctgtc
     1981 ctgaattccg ggctatccgg cgacttcggc gccgccccca ccaccatcct tcggatggtc
     2041 ccgctccccc tcgctacgct ccggggagta ccagttccag cttgggccgc tcccagctga
     2101 tgccgtcgcc gggcggggac acgccgatat cgaccgcgcc catgcgtcgg tagaaggcgg
     2161 ccgcttccgg gttcgagctg atcaccaccc gcttcgcgtc cagcgccgcc gcacgctccg
     2221 ccatatgcgc gaacagccgg cgaccgaccc ctatgccctg ggcgtcgttg gcggtgaaga
     2281 acaggtcaag ttcctggtcc ttcccaagcg ggatcagggc gtagaagccg gcgacggcgt
     2341 gaatgtcctg ggccagccag atctcgtggc cggcgtccgg cacggaccag ccggcggcga
     2401 agccgaagat catcgagcgg gcggccggat cctggtagcc gttgctgctc gccatcatcg
     2461 cccgcagggt cggcgtgtcc gcgggaacgg cccggcggat gatcaaaggc ccgcgctctc
     2521 gggcaggccc agcatcagat tgaggttctg cacggccgcg ccggaagcgc ccttgccgag
     2581 gttgtccagc agcgcaacca gccgcgcctg ccggccctcg tcagagccga acacgaacag
     2641 cttcatccgg ttggtgccgt tgaggccttc gggatcgagg gtcttgagcg tctcgatctc
     2701 cttgagcgac accacctcga cgaaggactc acccttgtag tggcgggtca gcacgtcatg
     2761 gatgtccctc aggtccgggc cattgcccat ctgggccagg tgcagcggaa cctcgaccag
     2821 catgccctgg gcgtagcggc cgaccgcggg ggtgaacagc ggacgggtca gcaggccgga
     2881 acggatctgc atctcgggca catgcttgtg ctccaggccg agcgcgtaga tgcggtacgg
     2941 aaccttcgtg aaggtcgggc cggcctcatc ctcgaactcg gcgatcatcg gcttgccgcc
     3001 gcccgtgtag ccgctgacgg cgttgacgct gacgggatgg tcggccggca tcaggccggc
     3061 ctggaccagg ggccggatca gggcgatcgc gccggtcgag tagcagcccg ggttggacac
     3121 gcgtttcgcg ctcttgatcg ccgcccgctg gctcttgcac agctcgggga agccgaaggt
     3181 ccagccctcg gccgtgcggt gcgcggtcga ggcgtcgatg accttgaccg ccgggttgct
     3241 gatcaggctg accgcctccc gcgccgcgtc gtccggcagg cagaggatca ccagatcggc
     3301 ggcgttcagc agctccgccc gggcctcggt gtccttgcgc cgttccggcg cgatcgacag
     3361 cagttcgagg tccgtacggc cggccagccg ctcacggatc tgaagcccgg tggtgccggc
     3421 ttcgccatcg atgaagacct tgggcgtgct catttcactc gttgctccac gttgcgtggt
     3481 tcgacacgcg acctccggtc gctgctctcc acgacgtata ttcttgcggc ccactcatgc
     3541 acgtcgtcct gagcaggccc gaagggccgt gtcgaaggac gcaccgcagt cccaacgcac
     3601 aaagaaaaag ggcgctccgg tttcccgaag cgccctttcg caaaccgtgt tcgcgaacga
     3661 ctagcgcttc gagaactgga aggagcggcg ggccttggcc ttgccgtact tcttccgctc
     3721 gacgacgcgg ctgtcgcggg tcaggaagcc gtgcggcttc agggccgtgc gcagcgcggg
     3781 ttcgtagtag gtcagggcct tggagatgcc gtgacgcacg gcgccggcct ggccgctgag
     3841 gcccgaacct tcaaccgtga cgatgacgtc gaactggccg acccggtcgg tgacctggaa
     3901 cggctgggcc agcatcatgc gcagcaccgg gcgggcgaag tagacttcct ggtcgcggcc
     3961 gttgatggtg atcgaaccct tgcccggctt gatccagacg cgggcgatcg cgttcttgcg
     4021 cttgccggtg gcataggcgc ggccgaactt gtccagcttc tggaccgccg gagcggcctc
     4081 gggctgggac gacaggctct ggagagcgtc gaaaccttga gcttcggcca tgatctattg
     4141 gctccggacg ttcttggcgt tcagttcggc gaacgcgatg ggctcagggc tctgcgcctg
     4201 gtgggtgtgc tcggcgccgt tgaagatgcg caggtgcgtc agctgcttgc gggccaaggg
     4261 gctttccttc ggcagcatgc gctcgacggc cttttccagc acgcgctcgg ggaaacgacc
     4321 ggccagctgc tttccggcgg tgatttcctt gatgccgccc gggtggccgg tgtggcggta
     4381 gtagatcttc tgctcgagct tgcggccggt gaacttcacc ttgtcggcgt tgaccacgac
     4441 gacgtagtcg ccgcaatcga cgttgggggt gtagtcagga cggtgcttgc cgcgaagacg
     4501 catggcgatg aacgacgcga ggcggccaac gaccacattc tcggcgtcga tcacgatcca
     4561 cttcttctcg acctcggcgg gcttcaacga agccgtggtc ttcatcatgg gactgatccg
     4621 ttgcaggcgg agacgaccgg tccccgacgt gagggcgcgc tgttacccgc gcagcacggg
     4681 gctgtcaaca ctcgcgcatg cgcaaaggac cggaaaccgt tacagcataa gggaaatcat
     4741 tcatgcggta acagaatacc cattcatcag ccctgcgctg tagtgacagc gtcacgaaca
     4801 gggcttagct ggcggccgtg cccggagtaa cgccatgacc ctgtcccgcc ggaacctgct
     4861 gagcgcgggc gccgtctccc tcgccttctc cggccttgcc cgggcccagg aagccccgga
     4921 gacctatcgc aacgagatca ccggctacgg gccgttgcgg gcggatccca acggggtgtt
     4981 cgatctgccg gaaggcttct cctaccgcgt ggtgtcgttc gccggccagt cgatgaacga
     5041 cggctatgtc gttccggcca atgccgacgg catggcctgc ttcccgggcc ggggatcgaa
     5101 ggtgatcctc atgcgcaacc acgagaacgg gctcaagcat cgcaacctcg gcccgctggg
     5161 catcggccga agcctgcgca agcggccgaa gccggacaag gtgttcgacg tcgacgccac
     5221 gggcgagcct ctttcgggcg gagtcagccg cctggtctac gatacggccg ccggccgcct
     5281 ggtgcgcgcc gagatggccc tgttcggcac ctcgaccaac tgcagcggcg ggccgacgcc
     5341 ctggggcagc tggctgacct gcgaggagac caccaccggg ccggcagagg gcctgggcaa
     5401 gccccacggc tgggtgttcg aggttcccgc ccgcgggcgc ggcctggtcg atccggttcc
     5461 gctcaaggcc atgggccgct tcttccacga ggccgcggcg gtcgatccgg tcaccggcat
     5521 cgtctatctg accgaggacg cccccgcccc ggaccgggcc ggcctgctct atcgctatct
     5581 gcccgacagc cctggccatc tggccgtcgg cgggcggctg caggcgctgg ccttccgcga
     5641 cgatccgggc gccgacgccc gcaactggga cggccgggtg atctggaaac agggtgatgt
     5701 gcgccaggcg gcctggatcg acctgaccga cgtcgagtcg cccggggacg acctgcgcca
     5761 ccgcggccac gccgccggcg cggtgatcct cggccgcggc gagggcctgt tccgcggccg
     5821 cgacggattc tacatgagct gcaccaacag cggtcccgcc aagcacggcc agatcctgcg
     5881 ttatcgcccc gggccccacg agggacagcc cggcgaggcg gacgccccgg gtacgatcga
     5941 gctgttcatc gaatccaccg acgatcgggt gctcgactat ccggacaacc tgacggtcgc
     6001 gccgaacggc cacctgctgg tctgcgagga ccgctactcg gcgacgctgc gcaaccatct
     6061 gcgcgccatc acgccggacg gccggagcta caccctcggc cgcaatgtct atcgcgacaa
     6121 tgccgaactg gccggcgtgt gtttctcgcc cgacggccag acgttgttcg tcaacatcta
     6181 ctggccaggg atcaccctgg cggtgaccgg cccctgggcg gcgctgagac cctagagccg
     6241 ccggacccgc caggcgaagg ccgtggctgt cgccagcagc gccaccgctc ccagctggtg
     6301 caggatgccc agccacagcg gcacgccggc catcagggtg gcgatgccca gcagcgcctg
     6361 cagcagcacg accccgccga ccgccagccc aaggccgcgc gcgcctggcg acaggaagcg
     6421 gtcgcgccac gccagggccg ccaccgtcag cccgccggcc agcagcagat aggcgcccag
     6481 gcgatggtgc agctgcaccg aggcctggtt gtgcgccagg gtcgcccaga ggctgtcgcc
     6541 ggcgtagtcg cgcgggaaca gccgtccgcc catcaacggc cagtcgttgt cgatgaagcc
     6601 ggcgtcggcg ccggcgacca gggccccgag caggcactgc aggaacaccg cagccaggag
     6661 aatcagggat ccggcccgcc agcgactggg attggcctgc cgcggctgtc cggcctcggc
     6721 gtcgagcgcg gtccagacca gcagcacgaa cagagccagg gccaggccca gatgggtcgc
     6781 cagccgctcc ggcgcgaccg agacccgcac ggccaggccg ctggcgacca tccaccagcc
     6841 cacggcgccc tgcaggccgc cgaggccgag catggctccg cagcgccaga tcagccggcg
     6901 cggaatgtcc ttgcggatga ggaagatcac aaaggggatg gcgaaggcgg cgccgaccag
     6961 cctgcccagc aggcgatgcg accattccca ccagtagatg gccttgaact cggccagact
     7021 catgccgcgg ttcagctgcg tgtactgggg aatttcgcgg tatttggcga attcgccttg
     7081 ccaggcctgc tcgcccatcg gcggcagggc gccggtgacc ggcttccatt cggtgatcga
     7141 caggcccgag cccgtcagtc gcgtcgcgcc gccgaccacc accatcgcca gcaccaggag
     7201 cgccaccgcc agcagccaga ccgcaaccgg tcgggaccgg tcggaacgca gaaacgacgt
     7261 catgacacct cttccacgaa cgcggagagc ctacggagcg cggttcccta cgccgcccgc
     7321 gcgacggggt ccatagcggt gacgccgacc gcgcgccctg tcatgctggc gacatggggt
     7381 cgccggggtt gttcatcgtc gtggtcatcg gcctgctggc gggggcgatc ggccgcgccc
     7441 tgctgggcgg ccgcccgtcc cggttcgccg gcttcagcgc cggagtcgcc ggcgcgatcg
     7501 tcggcgtccc tgccgccggc gccctcggct ggccggtcac aaccctctgg gctctggcgc
     7561 tggccgccct cggcggggcg gtcgtgtttc tggccgccgc gcgcctgctc gaccgacgct
     7621 gaccacggaa ccttgttccc cgacccggca tttggaggaa ccgggccttc aggaggaaaa
     7681 cgggcatgca cggactgaac tgggtcgtca tcatcatcgc tggcatcttc gccggctgga
     7741 tcgccgagcg gctgatgaac cgcaatcacg gcatcctgac caatctcatc gtcggcctgg
     7801 tcggcgcctt catcggcagc tggctcgccg gggcctttgg cttcgcctac agcggcctgc
     7861 tgccgagcat cctggtcgcc gtgctcggcg ccgttgtgct gctgttcgtg cttggcctct
     7921 tcaaacgccg ctgaggctgg cgtatccctc cggcggggct ctccggcctc gccggagccc
     7981 ccaatgccgc cgcggatcag aaagcttgtc ggactgctgg cgatgctcgt cttcctggtc
     8041 gcctgggcgg ccggggccgt ggccatcgcc gaccgcctgc ccgcccatcc ggccgtgagc
     8101 ctgctgttct tcctcgtcgt cggcacgggc tggggcctgc cgctgttccc gctgctgagc
     8161 tggatgaacc gggagccgaa gccgaagggc tgaagcgggc gacggtcggg gagaagatgg
     8221 tcggagcgac aggattcgaa cctgcgaccc cttgaccccc agtcaagtgc gctaccaggc
     8281 tgcgccacgc tccgacatct tctccggcgg atcgcgaacg accgccgtgg ggagcgccct
     8341 tatagggacc gcaccccggc ccggcaactc gaattcagcg acccttgagc agcccgtcca
     8401 ggcgggcctt gaccgcctcc agatcggcca gggcgaaggt cagccgatcc cgggcctcgc
     8461 cagccagatc gaccggttcg cgaggctccg gcaaggacgc ctgatcggcc ggcatggccg
     8521 tcccggcggc ctccccggcc gccgacaggc gccggacgcc ctgttccttg tgcagcttct
     8581 gcacgccctt gatggtgtag ccctcgtcat gcagcagggc gcgcacgccc cgcagcacgg
     8641 cgatgtcctg cggccggtag aaccgccggc cgccggcccg cttcatcggg cggatgaagg
     8701 agaacttggt ttcccagaag cgcagcacat gctggggcac gcccagctcc tcggctgctt
     8761 ccgagatcgt gcggaaggcg tgtgggccct tcgccaccgc ggccgtcagt cgctcagcgc
     8821 gcggtcgatc cgcgccttca tgacctgcga ggcgcgaaag ccgatcaccc ggcggggctc
     8881 gatctcggcc ggctcgccgg tcttgggatt gcgccccatg cgggcccgct tggcgcgcac
     8941 ctggaacaca ccgaagcccg agagcttgac ggtctcgccg cgctccagcg cctcggccac
     9001 caggtccagg gtgcgctcga cgagtccgcc acaatcctgg cgcgtcagcc ccacttcctc
     9061 gtggacggcc tcggtcaatt ccgcacgcgt gagggtttcg cccttcatac cccgctccca
     9121 gaccctgaaa accatcaggt gcatgggccg attggtccgt caagtcaaag ggttccccca
     9181 atgatcggat tcatggcgct agaggcggat cacacaggcg ccccaggtca ggccgccgcc
     9241 catggcctcg atcagcagca ggtcgccctt cctgatccgc ccgtcggcca tgccttcggc
     9301 ccaggccagc ggaattgagg cggccgaggt gttggcgtgg tcctcgacgg tctggatgac
     9361 cttgtgctcg tcgatcccca gccgatgggc cacgccctgc aggatccgct tgttggcctg
     9421 gtggggaatg aaccagtcga tgtcggaaac ctgcacgccg gcctgggcgg cggccgcggt
     9481 caccgcctcg gagatgttga tcaccgcgtg cttgaagacc tggttgccga gcatccgcag
     9541 cttgccgacc gtgccggtcg tcgacgggcc gccgtcgaca tagagcagtt cctgcttggt
     9601 cccgtcgcag cgcagggcga agcccagcag gccgcggtca gcggtggtcc cctgcccttc
     9661 gcccggctcg agcaccacgg cgccggcccc gtcgccgaac agcacgcagg tgccccggtc
     9721 ggtccagtcc atcagtcggg tcatggtctc ggcgccgatg accagggcgc atttcgcgtg
     9781 cccccgaccg acgaagccgt cggccaccgt cagggcatag acaaatccgg agcagaccgc
     9841 ctgcacgtcg aaggcgatgc cgaccgggca gccgagcttg cgctggacga tcgcggccgt
     9901 ggccggaaag gtcaggtcgg ccgtggtggt ggcgagaacg atcagatcga catccgccgc
     9961 ggtcttgccg gccgcggcca gggccttccg cgccgcctcg acggccaggt ccgacaccgc
    10021 ctggtcctcg ctcgcccgat gccggcggcg gatgcccgtg cgttcctgga tccaggcgtc
    10081 gttggtgtcg acgattttcg acagatcgtc attgctgacc acggtctcgg gcagatagcc
    10141 cccgacgccg gtcaccacac tacgcacaac actcacttgg ttactccctc cggcgaggcc
    10201 gtggcggtgg attcggacag ggtggccgtc aggcgctgca tgttgcggtc gatctcgccg
    10261 gcgaagtcgc tgcgggccag gtcggcggcg acgcggatgg cggcggcgta tccccgggcg
    10321 tcggcgccgc catggctctt gaccacgatg ccgttcagcc cgagcaacgg gccgccgttg
    10381 acggcccccg gatccatgcg ttggcgcatc ttcttcagcg cgccggaggc aaacagcgcc
    10441 cccagcatgg ccatagggcc cgaggtgaag gtcgcccgga gttcgctggc gaagaaccgg
    10501 gccaggccct cggcggtctt cagggcgatg ttgccggtga agccatcggt gacgatgacg
    10561 tcgaccgtgc ccttggcgat atcgttgccc tcgacgaagc cgcgatagtc gaggtcgaac
    10621 cgggtggcgc gcagcagggc gtgcgcctcg cgcacctcct cgtggccctt ctcctcctcc
    10681 gacccgacgt tgagcaggcc gaccgtgggc ctggccgagc cgtgcacggc gtgatggaag
    10741 gccgcgccca tgatggcgaa ctcgaccagt tgctcggcgt cgcactcgac attggcgccc
    10801 acgtcgagca ccgcggtgac gccgttgagc gtcggccagc tggcgacgat cgccgggcgg
    10861 gcgatgccgg ccgccatgcg caggaccagc ttcgagatcg ccatcagcgc gccggtgttg
    10921 ccggccgaaa cggcggcgag cgcttcgccg gccttgaccg attcgacggc gttccagacg
    10981 ctggacccct tgccgcgccg catcgcctgg gccggcttct cgtccatcga gatggcgcgg
    11041 tcggtgtgcc gcacggtgct ggcggcccgc gccgccggcg ttctggccag ctcggcgttg
    11101 atgcgggcct cgtcgccgtg cagcaggaaa cgcaactcag gcatggcccg ggcggcgatg
    11161 tcgacaccgg ggatgacgac ggacggaccg tggtccccgc ccatggcgtc aatggaaatg
    11221 acgatgttct ggggcaatgg ccggtcgcga ttccgttgcg gcccccgtat cggggcgaag
    11281 ccgccggaac ttacccccgc gcggcggcga tgcaagcccg gcctcgaccc gctattccgg
    11341 ggcgtcgtcc ttcttcagca gccgcaggac ggcgaagggc gacaggtcgg cctcgtcctt
    11401 cggcgcgatg aactccgccc ccggcttgcg cggaaaggga tcgagcgcca gggacaggtg
    11461 ctcgacgaca tagccgccca ggtcgatgtc ctcgccctcg agcacatccg gcggatcatc
    11521 ggcgtccgga tcgagatcga tttcctcggc atcctcctgc ggcgcgttgg ggctgccgga
    11581 tggcagggcg cgaaccagga attcgccctc ggcggtctcc tcgaagtcct cggcggtgac
    11641 gctgcaggtc tgggtgacgt cggcggaaaa ggcgccttcg atctcgaccc cgtccatcca
    11701 ggggcgaacc gtgacgtccg cgaccagcga tccgatcgac accagcccca gatgccgggc
    11761 gaccttcgtc cgcgtggctg cgtcggcttc cagatgtcgc gtcacgggcc cgcgcgccgc
    11821 ctcgccgaac cggatcacgt cccgccaggg
//
LOCUS       DAORNX010000037        11184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4374_length_11184_cov_7.024261, whole genome shotgun sequence.
ACCESSION   DAORNX010000037 DAORNX010000000
VERSION     DAORNX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4374_length_11184_cov_7.024261"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PLO65_02860"
     CDS             <1..1013
                     /locus_tag="PLO65_02860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27221.1"
                     /translation="VVAVSLKKAPFPAASAAVWRGGGLVWSEAFGMANLETRLPARPD
                     DRFRLASVSKVLTGAAVMRLAERGAIDLDVPIARYRPDLPAHHQATTLRQLASHQGGV
                     RHYIPRDFDPAAPGGPIDGRTYATTEDKLAIFIDDPLVSAPGEAVHYSSFGFVLLGAV
                     LESAVGKPFPAILADEVTGPLALASIAPEVRGRAVADRVSDYQPVYPALTDIVRCPPI
                     NPAYKWPAGGLLGAAPDVARFAGALTRPGYLGVAAMAQMLTGSPLRSGGAGPFGISWD
                     LDQDSRGRRRAWHAGSIAGGRSIIMMLPDEGAAVAVLTNLGQIDVDPLTPAQQIAEAF
                     LD"
     gene            1166..2896
                     /gene="ilvD"
                     /locus_tag="PLO65_02865"
     CDS             1166..2896
                     /gene="ilvD"
                     /locus_tag="PLO65_02865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD27222.1"
                     /translation="MARKPDGSWDKSKLPSRHVTEGPARAPHRSYYYAMGLGTREIAQ
                     PFVGVASCWNQAAPCNTTLMRQAEAVSVGVKKAGGTPREFCTITVTDGIAMGHEGMRS
                     SLVSRDAIADTVELTMRGHGYDALVGVAGCDKSLPGMMMAMLRLNVPSVFLYGGSILP
                     GRLDGKDLTVQDVFEAVGQFSAGTITEERLCQVEQHACPGDGACGGMFTANTMACVSE
                     AIGLALPLSSALPAAYEDRDAYAIASGEAVMRLIEKNIRPRDICTRKAFENAAVVVAA
                     TGGSTNGGLHLPAMAHECGVDFTLKDFAEIAARTPYIADLKPGGRFVARDMGEAGGVP
                     MLLKTLLEGGYLHGDVMTVTGKTLAENLADVVWRDDQEVIRPVSNPLSPTGGLVGLWG
                     NLAPEGCIVKVAGLAHQVHRGPARVFDGEDACFAAVEARDYKEGDVLVIRYEGARGGP
                     GMREMLSTTAALYGQGVSNIALITDGRFSGATRGLCIGHVGPEAAVGGPIALVQDGDI
                     INIDAHKGTIDIEVSDEELARRKAAWTPRETPYGNGAIWKFAQLVGPAHKGAVTHPGF
                     EGERHIYADI"
     gene            2900..3592
                     /locus_tag="PLO65_02870"
     CDS             2900..3592
                     /locus_tag="PLO65_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRD27223.1"
                     /translation="MKTIGLIGGVSWESTAYYYQQLNRMVRDRLGGRHSVKLVLVSVD
                     FAPVEAAAAAGLWDEVAGHLVAAAKQVEAGGADCLLIGDNTMHNVAADIRAATALPLI
                     HIGDATADALKAEGARRPLLLGTRYTMEMPFLRDHLSGRGVETVVPDKGARDELHRII
                     FDELVVGKFLPESKAAMLAMIEAGRREQGADAVIFGCTELGLLATPDDSALPVFDTAE
                     IHARAAIDFALA"
     gene            complement(3596..4000)
                     /locus_tag="PLO65_02875"
     CDS             complement(3596..4000)
                     /locus_tag="PLO65_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HRD27224.1"
                     /translation="MARTDTVDLPLPSPYERLGGAPALRKLVDAFYDAMDRDPAAADI
                     RRMHQPDLGPIRDKLFDWLSGWSGGPPLYNARPDRACIMSTHAPYVIGPAERDQWLAC
                     MRGALDNVAPEPDVRALFETAFDRLADGLRNR"
     gene            complement(4057..4743)
                     /locus_tag="PLO65_02880"
     CDS             complement(4057..4743)
                     /locus_tag="PLO65_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRD27225.1"
                     /translation="MTGLIAAAVFFVLLHLLVSGTGLRGAMVRRIGEGPYMGLFSLAS
                     VGGLVWLGWAYGQVKGAGPVYWDLGPGARHTAVALMLLALLLAVPGLLTPNPTSVKQE
                     GALDRPDAVKGMLRITRHPFLWGVAIWAFAHLLANGDLPGVILFGSLLVLAVFGTASI
                     DAKRRKALGAKWDAFAGQTSGVPFAAILGGKQTLALGEIGWWRLLLAIVVWAALLWGH
                     PILFGVNPLG"
     gene            complement(4801..5469)
                     /locus_tag="PLO65_02885"
     CDS             complement(4801..5469)
                     /locus_tag="PLO65_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27226.1"
                     /translation="MTDMARLFLLLGIAGAAVTFLGSLAIWFLDEPRRIRRALRRVLD
                     SEPESMVLARGRGRGAGFSFQTGLAAVAWDRGAWCLIYRLDELIGAELMVDGQVAARV
                     FRGEPRRALDQRVSSADQITLRLIFDDPRNPDFDLDLWLRGDEARRDDWSPRLAIQEA
                     NSWLARCEAILRRPLAPRPAPVVAPPPPPPVAGASATADDPPWDDVEDDEVDELDDAP
                     PRLL"
     gene            complement(5534..5905)
                     /locus_tag="PLO65_02890"
     CDS             complement(5534..5905)
                     /locus_tag="PLO65_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HRD27227.1"
                     /translation="MIKLTFCLTRRPDFTRETFQDYWFNTHAPLVASVRDVLRIRRYV
                     QMHSFPLEVSADTRASRGGPEGYDGVAELWWDSFEDMAATAGDPAAREAGRMLLEDEK
                     RFIDLTRSPLWWGEEREIFSA"
     gene            complement(5902..6681)
                     /gene="xth"
                     /locus_tag="PLO65_02895"
     CDS             complement(5902..6681)
                     /gene="xth"
                     /locus_tag="PLO65_02895"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRD27228.1"
                     /translation="MRLATWNVNSINARLETVLGWFEEASPDVAVLQEIKCVDEKFPA
                     EAFERLGYNIAVHGQKTYNGVAMLSKTPLEDVRRGLPGDDGDDQARYIEALVSGPRPV
                     RVAGIYLPNGNPVGTEKFAYKLAWMERLRTHAIALLRFEEPLVIAGDYNVIPRPDDAD
                     KPDGWLGDALFQPESRAAYRSLQNLGLTDAFMQADGGAGAYTFWDYQAGAWPRNHGIR
                     IDHALLSPQAADRLAGVEIHREVRGRDKPSDHVPVVVELNL"
     gene            6718..7056
                     /locus_tag="PLO65_02900"
     CDS             6718..7056
                     /locus_tag="PLO65_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27229.1"
                     /translation="MARGLKTTEMIDRRTRLILLVAATAACAGFMPLGAVGALFSPLI
                     FDARGNLLNPLAWLGFLLMIGFWIVCIVAPFVAWVLWRRQREPQAWAAISAPLVWGLA
                     TVTVLQFVPG"
     gene            complement(7038..8015)
                     /locus_tag="PLO65_02905"
     CDS             complement(7038..8015)
                     /locus_tag="PLO65_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27230.1"
                     /translation="MSLRLLAMAATITLAAGAARAAAPVETFLEIPGGPGPLKGTMLA
                     PAEPPPGPVVLILAGSGPTDRNGNNPLGVAGSSYRLMAEGLAARGVTSLRVDKRGMFA
                     SGAAAADANAVTVVDLAGDAHAWAAKLRAETGAPCVWLEGHSEGSLVALLAARDSRDI
                     CGLVLISAPGRRLSDVLREQLRSNPANAPLLDSALHAIDELEAGRTVDTAGMPAPLLN
                     LFHPRVQGFLIEMFRHDPAALAAGYDGPMLILQGDADLQVSVADAERLAAAHPGAKLV
                     ILPGVNHIMKTGSRDRAATRANYADPSLPLAPGVVEAIAAFVKAQPGTN"
     gene            complement(8024..8692)
                     /locus_tag="PLO65_02910"
     CDS             complement(8024..8692)
                     /locus_tag="PLO65_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRD27231.1"
                     /translation="MSRLPRPAVLIAAVGQAAAGVLLWRFGPHGPIPMHFSVGGTIDR
                     YGDRSEAALLVGGMALLTLAGGAALGWSGRRREADDARRRGLGVAQGVLIAATSAIVT
                     LQLAMATLGTDGTISPQRLSLIGICTILTVIGAAIGKVAPNALVGVRTPWSLNSRLAW
                     EKSNRLAGRLFFWGGLVGLVVAPALPEPQGHGLVIAGVLLAAAVCVFESWRVWRSDPQ
                     RRIV"
     gene            complement(8689..8997)
                     /locus_tag="PLO65_02915"
     CDS             complement(8689..8997)
                     /locus_tag="PLO65_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD27232.1"
                     /translation="MPMNSLFKALAHPARRRIIEMLRAGPLTSGDIAARFDMAWPTVT
                     GHLNTLKDAGLVDVEREGASMRYRLNISAVEEALAFLMEIAGAGSPLFAGRPVQEKSP
                     "
     gene            complement(9071..9403)
                     /gene="erpA"
                     /locus_tag="PLO65_02920"
     CDS             complement(9071..9403)
                     /gene="erpA"
                     /locus_tag="PLO65_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRD27233.1"
                     /translation="MTDTAVTLSEPAARRIRAIAEAEGRPLMLRVAVDGGGCSGFQYR
                     FDLVEAGEDDDLRIERDGVAALVDPFSLAMLKGSVIDFVDELASAEFRVRNPNARSSC
                     GCGVSFSI"
     gene            9483..10667
                     /locus_tag="PLO65_02925"
     CDS             9483..10667
                     /locus_tag="PLO65_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRD27234.1"
                     /translation="MPHPFSPPPRAPYAENPGLSRGRRHPEAESRTRTPFARDRDRII
                     HSTAFRRLKEKTQVFVAHEGDHYRTRLTHSLEVAQIARSISTALGLDNDLAETVALAH
                     DLGHPPFGHAGEDQLDDSMKPWGGFDHNVQTFRVVAKLEHRYPDFEGLNLTWETLEGV
                     VKHNGPVTGKLDRPSWSMVREFDAGWDLRLGSWASAEAQVAAIADDIAYNNHDVDDGL
                     QAGLFRLEELLDVPLIGPILHGVRAERPELDERLTRLEAVRRMIGAMIDDVMGETLRR
                     ASLTGVGSPDDVRGLAHALVAFSPDMLEDLARLRAFLMERMYRHWKVNRTRSQARRIL
                     ADMFSLFHAEPEVLPTEWFALSQNCDDGARARVVCDYIAGMTDRFAIEEHRRLFQMDT
                     WS"
     gene            10826..>11184
                     /locus_tag="PLO65_02930"
     CDS             10826..>11184
                     /locus_tag="PLO65_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRD27235.1"
                     /translation="MSDPDRGAYTPPTDSPLTFDARRPVRGGGPNSSTLMISAIILVL
                     LVAGAGFWLYKDGRRGADDQPRPVGTVVAETKAPPPAQAQPEDDSAGLQIYKQDDLAS
                     SAPTFAPGPEAVQPRPAP"
BASE COUNT         1743 a         3883 c         3959 g         1599 t
ORIGIN      
        1 cggtggtcgc cgtatcatta aaaaaggcgc cgttcccggc cgcttccgcg gcggtctggc
       61 gaggcggcgg actggtctgg agcgaggcct tcggcatggc gaacctggag acgcgactgc
      121 cggcgcggcc ggacgaccgg ttccgcctgg ccagcgtctc caaggtgctg actggcgcgg
      181 cggtgatgcg cctggccgag cggggcgcga tcgatctgga cgtcccgatc gcccgctaca
      241 ggcccgatct gccggcccac caccaggcca ccaccctgcg acagctggcc agccaccagg
      301 gtggggtgcg ccactacata ccgcgagact tcgacccggc cgccccgggc gggccgatcg
      361 acggccggac ctacgcgacc accgaagaca agctggccat cttcatcgac gacccgctgg
      421 tctccgcgcc gggcgaggcg gtccattatt ccagcttcgg cttcgtcctg ctgggcgcgg
      481 tgctggagtc agcggtcggc aagcccttcc cggcgatcct ggccgatgag gtcaccgggc
      541 ccctggccct ggccagcatc gcgccggaag tccgcggccg ggcagtggcg gaccgggtca
      601 gcgactacca gcccgtctac ccggccctga ccgatatcgt tcgctgcccg ccgatcaacc
      661 cggcctacaa atggccggcc ggcggcctgc tgggtgcggc cccggacgtg gcccgcttcg
      721 ccggcgccct cacgcggccc ggttatctcg gcgtggcggc gatggcgcag atgttgaccg
      781 gcagtccgtt gcgttcgggt ggggccgggc cgttcggaat cagctgggac ctcgaccagg
      841 acagccgggg ccgtcgccgg gcctggcatg cgggcagcat cgccggcggc cgctcgatca
      901 tcatgatgct gcccgacgaa ggcgcggcgg tggcggtgct gaccaacctg ggccagatcg
      961 acgtcgaccc gctgactccc gcccagcaga tcgccgaagc cttcctcgac tgaggggatt
     1021 cttcccgccc ggggcggcca ggggcctgcg ccgcgcacga ttgtcggacc ggaggcggtt
     1081 tgcggaaccg atttgtgccc cgcaggccct tccgcgcggc gccgcgctgc ggtaacccgc
     1141 aggcgcgtca tcgaggagta gagccatggc caggaagccc gacggaagct gggacaagtc
     1201 gaagctgccc agccgccatg tgaccgaggg cccggcccgc gcgccgcatc gctcctacta
     1261 ctacgccatg ggtctgggca cccgcgagat cgcccagccg ttcgtcggcg tggccagctg
     1321 ctggaaccag gcggcgccct gcaacaccac cctgatgcgc caggccgagg cggtcagcgt
     1381 cggggtcaag aaggccggcg gcacgccgcg cgagttctgc accatcaccg tcaccgacgg
     1441 catcgccatg ggccacgagg gcatgcgttc gtcactggtc agccgggacg ccatcgccga
     1501 caccgtcgaa ctgaccatgc gcggccacgg ctacgacgcc ctggtcggcg tcgccggctg
     1561 cgacaagtcg ttgcccggaa tgatgatggc catgctgcgg ctcaacgtgc cgagcgtgtt
     1621 cctctatggc ggctcgatcc tgccgggccg gctcgacggc aaggacctga cggtccagga
     1681 cgtgttcgag gccgtcggtc aattctcggc cgggacgatc accgaggaac gtctctgcca
     1741 ggtcgagcag cacgcctgcc cgggcgacgg ggcctgcggc ggcatgttca ccgccaacac
     1801 catggcctgc gtgtcggagg ccatcggcct ggccctgccg ctgtcgagcg ccttgccggc
     1861 cgcctacgaa gaccgggacg cctacgcgat tgcgtcaggt gaagccgtca tgcgcttgat
     1921 cgaaaagaat attcgcccac gtgacatctg cacgcgcaag gccttcgaga acgccgccgt
     1981 ggtggtcgcc gcgaccggcg gctcgaccaa cggcgggctg cacctgccgg ccatggccca
     2041 tgagtgcggc gtcgacttca ccctcaagga cttcgccgag atcgccgcgc gcacccccta
     2101 tatcgccgat ctcaagcccg gcggccggtt cgtggccagg gacatgggcg aggccggcgg
     2161 ggtgccgatg ctgctcaaga ccctgctcga gggcggctac ctgcacggcg acgtcatgac
     2221 cgtgaccggc aagaccctgg ccgagaacct cgccgatgtg gtctggcgcg atgatcagga
     2281 ggtcattcgg cccgtgagca acccgttgtc gccgaccggc ggcctggtcg ggctgtgggg
     2341 caacctcgcg ccggaaggct gtatcgtgaa agtcgcgggc ctggcccacc aggtccaccg
     2401 cggtccggcc cgggtgttcg acggcgaaga cgcctgtttc gccgccgtcg aggcgcgcga
     2461 ctacaaggaa ggcgacgtgc tggtcatccg ctacgagggc gccaggggcg gccccggcat
     2521 gcgcgagatg ctgtcgacca cggccgcgct ttacggccag ggcgtcagca acatcgccct
     2581 gatcaccgac ggccggttca gcggcgccac gcgcggcctg tgcatcggcc acgtcggccc
     2641 cgaggcggcc gtgggcggcc cgatcgccct ggtccaggac ggcgatatca tcaacatcga
     2701 cgcccacaag ggcacgatcg atatcgaggt cagcgacgag gagctggccc gccgcaaggc
     2761 cgcctggacc ccgcgcgaaa ccccctacgg caacggcgcc atctggaagt tcgcccagct
     2821 ggtcggtccc gcgcacaagg gcgcggtgac ccatccgggc ttcgagggcg agcgacatat
     2881 ctacgcggat atctagccga tgaagaccat cggcctgatc ggcggcgtca gctgggaaag
     2941 cacggcctac tattaccagc agctcaaccg catggtccgc gaccggctgg gtggccggca
     3001 ttcggtgaag ctggtcctcg tgtcggtcga tttcgcgccg gtcgaggcgg cggcggcggc
     3061 cgggctatgg gacgaggtcg cggggcatct ggtcgcggcg gcgaagcagg tcgaggccgg
     3121 cggcgccgac tgcctgctga tcggggacaa caccatgcat aacgtggcgg cggacatccg
     3181 cgccgcgacg gccctgccac tgatccatat cggcgacgcc accgcggacg ccctgaaggc
     3241 cgagggcgcg aggcggccgc tgctgctcgg aacccgctac accatggaga tgccgttcct
     3301 gcgcgatcac ctgtcggggc gaggcgtgga gacggtggtc ccggacaagg gcgcgcgtga
     3361 cgagctgcac cggatcatct tcgacgagct ggtggtcggg aagttcctgc cggaatcgaa
     3421 ggcggccatg ctggcgatga tcgaggcggg ccggcgcgag cagggcgccg acgcggtgat
     3481 tttcggctgc accgagctgg gcctgctggc gactccggac gactcggcgc ttcccgtatt
     3541 cgacacggcc gagatccacg cccgggcggc gatcgatttc gcgctggcct gaccctcagc
     3601 ggttgcgcag gccgtccgcc agccggtcga aggcggtttc gaacagggcc cgcacgtccg
     3661 gttccggcgc gacgttgtcc agggcgcccc gcatgcaggc cagccactgg tcgcgctcgg
     3721 ccgggccgat gacgtagggc gcatgggtcg acatgatgca ggcgcggtcg ggccgggcgt
     3781 tgtacagcgg cggcccgccg gaccagccgc tcagccagtc gaacagcttg tcgcggatcg
     3841 ggccaaggtc gggctggtgc atgcggcgga tgtctgccgc ggccggatcc cggtccatgg
     3901 cgtcatagaa ggcgtcgacc agcttgcgca gggccggcgc gccgcccagt cgctcgtagg
     3961 gagagggtag gggaaggtcg acggtatcgg ttctggccat gtgcaacggc tgccggatcc
     4021 cgcccggccg gtccatgacc aggatcaatc ctcgcgtcag cccagcgggt tgaccccgaa
     4081 caggatcggg tgaccccaca gcagggcggc ccagacgacg atggccagca acagtcgcca
     4141 ccagccgatc tcgccgagcg ccagcgtctg cttgccgccg agaatggccg cgaagggcac
     4201 gcccgaggtc tggccggcga aggcatccca ttttgcgccc agggccttcc ggcgcttggc
     4261 gtcgatgctg gccgtgccga acaccgccag cacgagcagc gaaccgaaca ggatcacgcc
     4321 cggcaggtcg ccgttggcca gcaggtgagc gaaggcccag atggccacgc cccaaaggaa
     4381 cgggtggcgg gtgatcctca gcatgccctt gaccgcgtcc ggcctgtcca gcgcgccttc
     4441 ctgtttgacg ctggtcgggt tcggcgtgag cagcccggga accgccagca gcagggccag
     4501 cagcatcagg gccaccgccg tgtgcctggc gccggggccg agatcccaat agacaggccc
     4561 ggcgcccttg acctgtccat aggcccagcc cagccacacc aggccgccga ccgaagcgag
     4621 ggagaacagt cccatgtagg gaccttcgcc gatgcgtcga accatggcgc cgcgcaaacc
     4681 ggtgcccgag accagcagat gcagcaggac gaagaagacg gcggcggcga tcaggccggt
     4741 catgacaatc cctcccaggg tcggcgggag aagcctgcgc ccatcaattt acgctgtcaa
     4801 ttatagaagg cgtggcggcg cgtcgtcgag ctcgtcgact tcgtcgtcct cgacgtcgtc
     4861 ccatggcgga tcgtcggccg tcgcgctggc cccggcgacg ggaggcggcg gcggcggggc
     4921 gacgacagga gcgggccggg gcgcgagcgg ccgacggaga atggcctcgc accgggccag
     4981 ccagctgttg gcctcctgga tcgccagccg gggtgaccag tcgtcacgcc gcgcctcgtc
     5041 gccgcgcagc cacaggtcca ggtcgaaatc cgggttgcga ggatcatcga agatcagccg
     5101 cagggtgatc tggtcagcgg aggagacgcg ctgatccagc gcccgccggg gctcgccgcg
     5161 aaagacgcgc gcggcgacct ggccgtcgac catcagttcc gccccgatca gctcgtcgag
     5221 ccggtagatc aggcaccagg ccccgcgatc ccaggcgacg gcggccagtc cggtctggaa
     5281 cgagaaaccc gcgccacgtc cgcggccacg cgccagcacc atggattccg gctcgctgtc
     5341 cagcacccgg cgcagggcgc ggcggatgcg ccgcggctcg tcgaggaacc agatggccag
     5401 gctgccgagg aaggtcacgg cggcgccggc gatcccgagg agcagaaaca ggcgggccat
     5461 gtcggtcatg gcggcaggct agcgggagtc ccgggcaacg tcagcccttg cgcgccgggc
     5521 ccggatgaga cgatcaggcc gaaaagatct cccgctcctc gccccaccac agcggcgagc
     5581 gggtcaggtc gataaagcgc ttctcgtcct cgagcagcat gcggccggcc tcgcgcgcgg
     5641 ccggatcgcc ggccgtggcg gccatgtcct cgaaactgtc ccaccagagt tcggccacgc
     5701 cgtcatagcc ctcggggccg ccccgggatg cgcgggtgtc agcgctgacc tcgagcggga
     5761 agctgtgcat ctgcacataa cggcgaatgc gcagcacgtc cctgacgctg gcgaccagcg
     5821 gcgcgtgggt gttgaaccag tagtcctgga acgtttctcg cgtgaagtcc ggacggcggg
     5881 tcaggcagaa ggtcagcttg atcacaggtt cagctccacc acgaccggca cgtggtccga
     5941 aggcttgtct cgcccgcgca cctcgcgatg gatctccacg cccgcaagcc ggtcggcggc
     6001 ctggggagag aggagggcat gatcgatgcg gatgccatgg ttccgcggcc aggcgccggc
     6061 ctggtagtcc cagaaggtgt aggcgcccgc gccgccgtcg gcctgcatga aggcgtcggt
     6121 caggcccagg ttctgcagcg accggtaggc ggcgcggctc tccggctgga acagcgcgtc
     6181 gccgagccag ccatcgggct tgtcggcgtc atcggggcgg gggatgacat tgtagtcgcc
     6241 ggcgatgacc agcggctcct caaagcgcag cagcgcgatg gcgtgggtcc gcaggcgctc
     6301 catccaggcg agcttgtagg cgaacttctc ggtgcccacc gggttgccgt tgggcaggta
     6361 gatgccggcc acgcgcacgg gccggggtcc tgacaccagc gcctcgatgt agcgggcctg
     6421 atcgtcgccg tcgtcgccag gcagaccccg gcggacatcc tcgagcgggg tcttcgagag
     6481 catggccacg ccgttgtagg tcttctggcc gtggacggcg atgttgtagc ccagccgctc
     6541 gaaggcctcg gcggggaact tctcgtcgac gcacttgatt tcctgcagca ccgcgacgtc
     6601 gggcgaggct tcctcgaacc agcccaatac agtttccagg cgagcgttga tcgagttcac
     6661 attccaggtg gcgaggcgca ttgacggctc cggctgcggc gcgcgaggct agggcccatg
     6721 gcgagagggc tcaagaccac cgagatgatt gatcgccgga cccggctgat cctgctggtc
     6781 gcggcgacgg cggcctgcgc cggcttcatg ccgctcggcg cggtcggggc gctgttctcg
     6841 ccgctgatct tcgatgcccg cggcaacctg ctcaaccctt tggcctggct gggattcctg
     6901 ctgatgatcg gcttctggat cgtctgcatc gtggcgccgt tcgtggcctg ggtcctgtgg
     6961 cgtcgccaga gggaaccgca ggcctgggcc gcgatcagcg cgccgctggt ctgggggctg
     7021 gccacggtga ccgtgcttca gttcgtaccg ggctgagctt tcacgaaggc ggcgatggcc
     7081 tcgaccaccc ccggcgccag cggcagggac gggtcggcgt agtttgcgcg ggtcgccgcc
     7141 cggtcgcggg atccggtctt catgatgtgg ttgacgccgg gcaggatcac cagcttcgcg
     7201 ccggggtgcg cggccgccag ccgttcggcg tccgccaccg agacctgcag gtcggcgtcg
     7261 ccctgaagga tgagcatggg cccatcgtag cccgccgcca gcgccgccgg atcatggcgg
     7321 aacatctcga tcaggaagcc ctgcaccctg gggtgaaaca ggttgagcag gggggcgggc
     7381 atgccggcgg tgtcgaccgt ccggccggct tccagctcgt cgatcgcatg cagggccgaa
     7441 tcgagcagcg gggcgttggc cggattcgac cgcaactgct cgcgcagcac gtcgctgagc
     7501 cgacgaccag gcgctgaaat caggaccagt ccgcagatgt cccggctgtc gcgcgccgcc
     7561 agcagggcca ccaggctgcc ctcgctatgg ccttccagcc agacgcaggg cgcgccggtc
     7621 tcggctcgca gcttcgccgc ccaggcatgg gcgtcgccgg ccaggtcgac gacggtcacg
     7681 gcgttggcgt cggccgccgc cgcgccgctg gcgaacatgc cgcgcttgtc gacccgcagg
     7741 ctggtcacgc cacgcgcggc cagtccctcg gccatcagcc ggtagctcga accggcgacg
     7801 ccaagcgggt tgttgccgtt gcggtcggtc gggccggagc cggccaggat cagcacaaca
     7861 ggtccggggg ggggctccgc cggggccagc atcgtaccct tcagagggcc aggtccgccg
     7921 ggaatctcga ggaaggtctc caccggggcg gcggcccggg cggcgccggc ggcgagggtg
     7981 attgtcgccg ccatggcgag aagacgaagg gacatgcctg ctcctagacg atgcggcgct
     8041 ggggatcgga acgccagacg cgccagcttt cgaagacgca gacggcggcg gcgagcagga
     8101 cgccggcgat gacaagaccg tggccctgtg gctcgggcag cgccggcgcg acgaccaggc
     8161 cgacgagccc gccccagaag aacaggcggc cggcgaggcg gtttgatttc tcccaggcca
     8221 gccggctgtt gaggctccag ggcgtgcgca ccccgaccag ggcgttgggc gcgaccttgc
     8281 cgatggcggc gccgatgacc gtcaggatgg tgcagatgcc gatgagcgac aggcgctgcg
     8341 gcgaaatggt cccgtccgtt cccagggtcg ccatggccag ttgcagggtg acgatcgcac
     8401 tcgtcgcggc gatcagcacc ccctgggcga cgccaagccc gcggcggcgg gcgtcgtcgg
     8461 cctcacgccg ccgaccactc cagcccaggg ccgcgccgcc ggccagggtc agcagcgcca
     8521 tgccgccgac cagcagggcg gcctcgctcc ggtcgccata gcgatcgatc gtccccccga
     8581 cgctgaaatg catcgggatc ggtccgtggg gtccaaaccg ccacaggagg acacccgccg
     8641 ccgcctggcc aacggcggcg atcaggacgg cggggcgggg aaggcggctc atggggactt
     8701 ctcctggacg ggacgcccgg cgaagagggg cgacccggct ccggcgattt ccatcaggaa
     8761 ggccagggcc tcctcgacgg cggaaatgtt caggcggtag cgcatcgagg ccccctcgcg
     8821 ctcgacgtcg accaggccgg cgtccttcag ggtgttgaga tggccggtga cggtcggcca
     8881 ggccatgtcg aaccgcgcgg cgatgtcgcc ggaggtcagc ggcccggctc gcagcatctc
     8941 gatgatgcgg cggcgagccg ggtgcgccag ggccttgaac agcgagttca taggcatgaa
     9001 gctaattagc gatttcgcta atttcaagcg ccaatcaagc ggcgctccgg gggcgccgtc
     9061 atcgtcgatg tcagatggaa aagctgacgc cgcagccgca gctggatctg gcgttcggat
     9121 tgcggacgcg gaactcggcg ctggccagtt catcgacgaa atcgatcacc gaccccttga
     9181 gcatggccag cgagaacgga tcgaccaggg ccgcgacccc gtcccgttcg atgcgcagat
     9241 cgtcgtcctc gccggcttcg acgaggtcga agcggtactg gaaacccgag catccgccgc
     9301 cgtcgaccgc cacgcgcagc atcaacggac ggccctcggc ctccgcgatg gcgcgaatcc
     9361 ggcgggcggc gggctctgac agggtgacgg cggtatcagt catggctgtg gaaaactctc
     9421 tcccgtccta tatgtgcaac cccgcgcccc gggaaaaggg gcgatcgaca ctgggccgct
     9481 cgatgccgca tccgttctcg cctccgccgc gcgcgcccta tgccgagaat cccggcctgt
     9541 cccggggccg gcggcatccc gaggcggaga gccgcacgcg cacgccgttc gcccgcgacc
     9601 gtgaccggat cattcacagc accgccttcc gtcggctgaa ggaaaagacc caggtcttcg
     9661 tggcccacga gggggaccac taccgcacgc ggctgaccca ctcgctcgag gtggcccaga
     9721 tcgcccggtc gatctccacc gcgctggggc tcgacaacga cctggccgag accgtcgccc
     9781 tggcccacga ccttggccac cccccgttcg gccacgccgg cgaggaccag ctggacgaca
     9841 gcatgaagcc gtggggcggc ttcgaccaca atgtccagac cttccgggtg gtcgccaagc
     9901 tcgagcatcg ctatcccgat ttcgagggcc tgaacctgac ctgggaaacg cttgaaggcg
     9961 tggtcaagca caatggcccg gtgaccggga agctcgaccg gccatcctgg agcatggtgc
    10021 gtgagttcga cgccgggtgg gacctgcggc tcggcagctg ggcgtcggcg gaagcccagg
    10081 tcgcggcgat cgccgacgac atcgcctaca acaaccacga cgtcgacgac gggttgcagg
    10141 ccggcctgtt ccggcttgag gaactgctcg acgtacccct gatcggtccg atcctgcacg
    10201 gcgtgcgggc cgaacggccc gaactcgacg agcggctgac ccggctggag gccgtgcgcc
    10261 gcatgatcgg ggcgatgatc gacgacgtga tgggcgagac cctgcgccgc gcctccctga
    10321 ccggcgtcgg ctcgccggat gacgtgcggg gtctggctca cgccctggtg gccttttctc
    10381 ccgacatgct cgaggacctg gcccgcctgc gcgcctttct gatggaacgg atgtatcgcc
    10441 actggaaggt caaccggacc cgcagccagg ccaggcgtat cctggccgac atgttctccc
    10501 tgttccacgc cgagccggaa gtcctgccca ccgagtggtt cgccctgtcg cagaactgcg
    10561 acgacggggc ccgggcccgc gtcgtgtgcg actacatcgc cggcatgact gaccggttcg
    10621 ccatcgagga acaccggcgg ctgttccaga tggacacctg gagctgagcg cggcgcgcgg
    10681 cgttaacggt tcggcaaccg caaattaacc gttcggtagc gatcttgccg ctctggcggc
    10741 cgcgcgccgc gcttcggtag actgggcccc gtcggcgcgc gattcgccgc gatgtccgat
    10801 ccgccgctcc ctggagccct gcttgatgtc cgatcccgat cgcggcgcct atacgccccc
    10861 gaccgactcc ccgctgacct tcgacgcgcg gcggcccgtg cgcggcggcg gacccaactc
    10921 ctcgaccctg atgatcagcg cgatcatcct cgtcctgctg gtcgcggggg ccgggttctg
    10981 gctctacaag gacggccgac gcggcgccga cgaccagccc cggccggtcg ggacggtggt
    11041 cgccgaaacc aaggcccccc cgcccgctca ggcccagccc gaggacgaca gcgccggcct
    11101 gcagatctac aagcaggacg acctggccag cagcgcgccg accttcgcgc cggggccgga
    11161 ggcggtccag ccgcgccccg cgcc
//
LOCUS       DAORNX010000038        11151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4398_length_11151_cov_6.763248, whole genome shotgun sequence.
ACCESSION   DAORNX010000038 DAORNX010000000
VERSION     DAORNX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_11151_cov_6.763248"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLO65_02935"
     CDS             complement(<1..392)
                     /locus_tag="PLO65_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27236.1"
                     /translation="MIIRKLAPAIALAALMLAAAAGLRYAEGLGLLGPDGAKRTTQVL
                     IGLMLAGYANLMPKQIGKAGASPLALARTQSALRVGGWSLTLGGLVYAGLWALAPLPV
                     ADTLSMIVVAGATALTAGYALWSFMACRG"
     gene            complement(389..709)
                     /locus_tag="PLO65_02940"
     CDS             complement(389..709)
                     /locus_tag="PLO65_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRD27237.1"
                     /translation="MNKVFKALADPTRRRVLELLRRRPMTAGELSDQFPVSKPTMSAH
                     FAVLREADLIDAHKQGATITYQLKLSVLEDALLGFSQVFGIGVGNAGPGGESAPESTK
                     GTPS"
     gene            complement(915..2564)
                     /locus_tag="PLO65_02945"
     CDS             complement(915..2564)
                     /locus_tag="PLO65_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRD27238.1"
                     /translation="MSGWNFADVWEEIAAAAPDRPAQIRGQRVISWGDFDRRANALAA
                     HLLKAGLGHQAKVAAYLYNAPEYLETYYAAFKASLAPVNTNYRYETDELLYLFDNADA
                     EAIVFHAGFAPKLEAIRDRLTKVKCWIAVADGAAPIPAWATDYESIAAPGADRVAGPR
                     GRSGDDLLLLYTGGTTGMPKGVMWRQDDLFQVLGAGGNVLAMIPPLETPHEAGVRVRT
                     GRAGDLHLPPPTTMIAACPLMHGTAQFGSFGALTGGGCIITLPSQRFSAEELWDEVHR
                     LRANSISIVGLAFAAPMLEALEANPGRWDLGCVTRIGSSGTVWSYDNKQALLKHLPPG
                     AMLMDSLGSSEAVGLGGSTSVAGAEATTAQFLVGPNAAVFKEDGTRVEPGSGERGLVA
                     VGGFIPVGYYKDPEKSARTFQTFEGRRWSVPGDFAEVNADGTIKLLGRGSQVINTGGE
                     KVFPEEVEEALKTHPLVRDAVVVGVPDARFGERICAVVDVGGNVAPPLTELSAHVRGR
                     LADYKAPRELVVTTVHRAANGKVDYKAVRAQALAALGVTTA"
     gene            complement(2669..3106)
                     /locus_tag="PLO65_02950"
     CDS             complement(2669..3106)
                     /locus_tag="PLO65_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRD27239.1"
                     /translation="MKPFAIAAALTLLAAPVLAASSTIEVAQAWSRPAPQGGNGAGYA
                     VITNHGATADTLVAASSPVAARIEIHESMIMGGQAMMHPRPGGLPVPAGATVALKPGG
                     WHMMLMGLKQPLKAGDQFPATLTFRKAGRMTVQFSVQATAPAN"
     gene            complement(3129..3605)
                     /locus_tag="PLO65_02955"
     CDS             complement(3129..3605)
                     /locus_tag="PLO65_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRD27240.1"
                     /translation="MRLAPLLAALLLAACSPAKPPAYSSAGIAVAEPFSRPAPAGGTG
                     AGFFSVTNNNSGPDVLIAVESPVAARVQMHETSTAGGMMRMEELKDGVRFKAGETVDF
                     KPGGKHVMFIDLARPLKAGDRIPATLVFRNAGRAPIELVVRGAPSRGMAGMANHDH"
     gene            complement(3624..>4397)
                     /locus_tag="PLO65_02960"
     CDS             complement(3624..>4397)
                     /locus_tag="PLO65_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRD27241.1"
                     /translation="RVWWRDLHAVTGAFACAILLFLAVTGMPWSGYWGENFRRLVNDS
                     GLGMPAGLKATTVASDAALAATRAGSWTMDRAAVPTVPAATLRPLPIAAIMAEASRRG
                     VAPGYVVRLPGSAGGVYTIQSYPRQATGQRVIHLDQYTGRVIADLGFRDYGPVSKAVE
                     WGIAVHTGGQFGRVNQLVMLAGCLAIVTLSISAAVMWWRRRPAGRLAAPPAQPRGLSA
                     FALFALAIGLVLPLLGLSILLAFAVDGLASNTLKLKHGL"
     assembly_gap    4398..4444
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4445..4951)
                     /locus_tag="PLO65_02965"
     CDS             complement(<4445..4951)
                     /locus_tag="PLO65_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRD27242.1"
                     /translation="MSAEEPPPGASRLYRTIWRWHFYAGLICLPFFILLASTGALYLF
                     RDDLAPLLEHRRLVVEATAAPSLPPEVLIARALAAQPGTPIRFTPPPAPDRSAEVGIR
                     DGRGEVVSVFLVPSTGRVLDRVADDQRPMTLISHIHSLEILGKAPNLVIEVVAGWAIL
                     LVASGVFLW"
     gene            complement(5001..7058)
                     /locus_tag="PLO65_02970"
     CDS             complement(5001..7058)
                     /locus_tag="PLO65_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27243.1"
                     /translation="MTMNQGARLRAMLLGASCLAAPALAHATDAPTTVDSVIVTANPD
                     PEDPAVVADARRRLSRTPGAVSVISAESFTSRESLAFDDMLRDAPGVYAQRKWGGDVR
                     ISIRGSGIGNANHNRGLLLAQDGVPLNEADGYGDSQIADPLSTRFIEIYRGGNALRFG
                     GSLLGGAINMVTPTGATAGFEQQVRIDGGSYGLLRENIALARRSDDWDVYVSGTNQTA
                     QGWRSQSQQNIQFGSLNLGRSFGEDREVRLIVNGSNINQEIPGALTRSQFQADPSQTA
                     PANYANDQGRNQRGLRASLRTRWRLGDSTLFEGAVYGLWKDLDHPIFQVIDQQSRNWG
                     AFGRLDWQGEIAGMKADAWAGLWLRTGDLDSNFYVNVKGHRGAPRSRTLQNADAADLF
                     AEGRLFVSDHLALVGGGTWGRAGRDYRSFALPGVAGTFNLTASRDYDWFAPRVGLLWQ
                     AEDGAQLFANLTKSVEPPNFGSMSPTGTGFAPVEAQEAWTAEAGARGRRGPFTWDVTL
                     YRAVLDREMLQYTVAPGIPASTFNADRTIHQGLEAAVDWRFLPRWRLRQTWTWSDFRF
                     DGDSQYGDNRLPIVPEHFYRAELRYDAPSGWFIAPSVEWSASDIEVDYANTTTAPAYA
                     IANLNAGWAVRDGLSLFVDARNLTDEAYVSNVQAAVLAGPATAAYWPGDGRSLFAGLT
                     VSF"
     assembly_gap    7290..7364
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7584..>7677
                     /locus_tag="PLO65_02975"
     CDS             7584..>7677
                     /locus_tag="PLO65_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27244.1"
                     /translation="MAAEVSPGEYKDVVLFLATAGVVVPLFRRWR"
     assembly_gap    7678..7687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7688..9372
                     /locus_tag="PLO65_02980"
     CDS             <7688..9372
                     /locus_tag="PLO65_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRD27245.1"
                     /translation="ILGFLGAGVVLGPSGLGALAGSLPWLGAVTIDNPKDVAQLAEFG
                     VVFLLFMIGLELSWERLRLMRRFVFGMGALQVALCSAAIATIATLLGQPLQAAVAIGA
                     ALALSSTAMVMPLLAESKRQHSQAGRATFAVLLFQDLAVAPILITLTVIGSQGGGDLF
                     SPKMLLTFAPAALGLLALVVFGRLLLRPMMKSVARARSEELFMAASLLIVIGAGLVAA
                     LTGLSMALGAFVAGLLLAETEYRHEVEVTIEPFKGLLLGLFFLSIGIGLDLSLLFQKP
                     ALILGMALGALILTGGVVFLLGLLFGLKPRKALEAGLLLAAGGEFAFVILDTAMNSGL
                     VDREVGRTVLVASTLTMFCIPALAALGARLGGRPGANPATSTPVEPPLSTDEPAVLVV
                     GYGRVGRLVGDMLGRHDIRWVAAERDPKLVEAGRRAGVAIFYGDASRAEFLRRCGLDS
                     APAVVVTMDNPEGAEVVVATARELRPDLTIVARARDARHAKRLYELGATDAVPETIEA
                     SLQLSEAALVDIGVPMGLVIASIHERRDEFRSELNRPEALGGRRRLRDGTKP"
     gene            complement(9406..>11151)
                     /locus_tag="PLO65_02985"
     CDS             complement(9406..>11151)
                     /locus_tag="PLO65_02985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRD27246.1"
                     /translation="TDGRKPGGLKGTLAGARRAFGGAGGRALSIVGDRFSITGEDGVF
                     LCSAKVRERVAARSAARAMAATAPAGSTIGAVRPALGLAAFADVKGDLRPELFRMEQT
                     QAHLQRIVDRIASAWPYPDAPRPRVLVSAGMAFNAMATADNTIVVMVGIFAPGEDETE
                     SGLRDTDLYWLLAHEYAHIALGHTDKAESIYGQVRLLNSMATAYMSGAQLTSSLQYSQ
                     ANVQDPELGDRIGIAQRAHDNLRFITKSMVEPAWGRTNEDEADTAGTDLITSLGYYPQ
                     FAYTAIRFEKSDKAWSDRVKAAQASIDKRVGGLVSDPKFQSAVDAGDTGAAFGAVGES
                     IKKGLYDFARQWLLDYLTRDHRSAEAREQGLDRYKETAYVKAGLTPEPRPLREEVDSL
                     VRSAEVREALVAAAAAAACQDALANADVAAARTEITKAMATRYRREAYIRFWAARVAV
                     DSGDLAGAITHLEAARVAPNISPMAYRELAKLYTGSGQVTKARTIIAEGRTRTGDSDY
                     FLPEDVRIAARAGDRAVIATLMKACRATRREQIAIECTEALLDLDWEKLTPAARKEAM
                     QYARWGELGLIATDR"
BASE COUNT         1555 a         3724 c         4063 g         1677 t
ORIGIN      
        1 ccgcggcagg ccatgaacga ccagagcgcg tatccggccg tcagggccgt cgccccggcg
       61 accacgatca tcgagagcgt gtcggcgacc ggcagcggcg cgagggccca cagaccggcg
      121 tagacaaggc ccccgagcgt cagcgaccag ccgccgacgc gcagcgccga ctgggtccgg
      181 gccagcgcca gcggcgaagc gccggccttg ccgatctgct tgggcataag gttggcgtag
      241 ccggccagca tgaggccgat caggacctgc gtggtccgct tcgccccgtc cgggcccagc
      301 aggccgaggc cctcggcgta gcggaggccg gcggcggccg cgagcatgag ggccgcgagg
      361 gcgattgccg gggcaagctt tctgatgatc atgacggtgt tcccttcgtg gattcggggg
      421 cggactcgcc ccccggcccg gcgttcccga cgccgatgcc gaagacctgt gaaaagccca
      481 gcagcgcgtc ctcgagcacg gacagcttga gctggtaggt gatggtcgcg ccctgtttgt
      541 gggcgtcgat caggtcggcc tcgcgcagca cagcgaaatg ggccgacatg gtcggcttcg
      601 agacggggaa ctggtcgctc agctcgccgg ccgtcatcgg ccggcgcctc agcagttcga
      661 gcacccggcg gcgggtcgga tcggccaggg ccttgaagac cttgttcatg ctcgtcattt
      721 agctaaacat cgaaattatg tcaaacggca cgggatcctc ccctcgacag aggggagggg
      781 caccagccgg aggctggtgg aggggcagcg cgaacgcggc ggtcccaccc tggattcagg
      841 acgatccggc gaggcctggg ctgacccctc caccacgctc cgcgtggtcc ccctcctcac
      901 cttgtgggga ggactcaggc ggttgtaacc cccagcgccg ccagggcctg ggcgcgcacc
      961 gccttgtagt cgaccttgcc gttggcggcg cggtggacgg tggtcaccac cagttcgcgt
     1021 ggggccttgt agtccgccag ccggccacgc acgtgggcgg acagttcggt cagcgggggg
     1081 gcgacgttgc cgccgacgtc gaccaccgcg cagatgcgct cgccgaaacg ggcgtccggc
     1141 accccgacga ccaccgcgtc gcggaccagc gggtgggtct tgagcgcttc ctcgacctcc
     1201 tcggggaaga ccttctcccc gccggtgttg atcacctgcg agccgcggcc cagcagcttg
     1261 atcgtcccgt ccgcgttgac ctcggcgaag tcgcccggga ccgaccagcg gcggccctcg
     1321 aaggtctgga aggtcctggc cgacttctcg ggatccttgt agtaaccgac cgggatgaag
     1381 ccgccgacag ccaccaggcc gcgctcgccg ctgcccggct cgacgcgggt cccgtcttcc
     1441 ttgaacaccg cggcgttggg gccgacgagg aactgggcgg tggtcgcctc ggcgcccgcc
     1501 accgaggtcg agccgcccag gccgaccgct tccgacgaac cgaggctgtc catcagcatg
     1561 gccccgggcg gcagatgctt cagcaaggcc tgcttgttgt cgtagctcca gaccgtgccc
     1621 gacgagccga tgcgggtgac gcagcccagg tcccagcggc ccgggttggc ctcgagcgcc
     1681 tcgagcatcg gcgcggcgaa ggccaggccg acgatggaga tgctgttggc ccgcaggcgg
     1741 tggacctcgt cccacagctc ctccgcggag aagcgctgcg agggcagggt gatgatgcag
     1801 ccgccgccgg tcagggcgcc gaagctgccg aactgcgccg tgccatgcat cagcgggcag
     1861 gccgcgatca tcgtcgtggg cggcggcagg tgcaggtcgc cggccctgcc tgtgcgcacc
     1921 cgcaccccgg cctcgtgcgg tgtctccagc ggcgggatca tcgccagcac gttgccgccg
     1981 gcgccgagca cctggaacag gtcgtcctgg cgccacatga cgcccttggg catgccggtc
     2041 gtgccgccgg tgtagagcag cagcaggtcg tcgcccgagc ggccccgcgg tccggcgacg
     2101 cggtccgcgc cgggcgcggc gatgctctcg tagtccgtgg cccaggccgg gatcggcgcg
     2161 gccccgtcgg ccaccgcgat ccagcacttc accttggtca ggcgatcgcg gatggcctcg
     2221 agcttgggcg cgaagccggc atggaagacg atcgcctcgg cgtcggcgtt gtcgaacaga
     2281 tagagcagct cgtcggtttc gtagcggtag ttggtgttga ccggcgcgag gctggccttg
     2341 aaggcggcgt agtaggtctc gaggtactcg ggcgcgttgt agagataggc cgcgaccttg
     2401 gcctgatggc cgaggccggc cttcagcaga tgggcggcca gggcgttggc ccggcggtcg
     2461 aaatcgcccc agctgatgac ccgctggccg cggatctggg cggggcggtc cggcgccgcc
     2521 gcggcgatct cttcccagac atcggcgaaa ttccagccgg acatgcgcgc tctcccgaat
     2581 gcgcctctga ccgggcgccc acgcaagtta gcggcgttca ggaaggccct gtcaacgcgt
     2641 gacccggcgt cagcggcctg tccgtccgtc agttcgccgg cgcggtcgcc tggaccgaaa
     2701 actgcaccgt catcctgccg gccttcctga aggtcagggt cgccgggaac tggtcgccgg
     2761 ccttcagcgg ctgcttcagc cccatcagca tcatgtgcca gccgccgggc ttgagggcga
     2821 cggtcgcgcc ggccggaacc ggcaggccgc cgggccgcgg atgcatcatc gcctgtccgc
     2881 ccatgatcat cgactcgtgg atctcgatgc gggcggcgac cgggctgctg gcggcgacca
     2941 gcgtgtcggc ggtcgctcca tggtttgtga tcacggcgta gccggcgcca ttgccgccct
     3001 ggggggcagg gcgggaccat gcctgggcga cctcgatggt cgaggaggcg gcgagcacgg
     3061 gggcggccag cagggtcagg gcggcggcga tggcgaaggg cttcatgggt ggctccggga
     3121 agtggcggtc agtggtcgtg attggccatg ccggccatgc cgcgggaagg ggctccgcgc
     3181 accaccagtt cgatcggcgc gcgcccggca ttgcggaaca ccagcgtggc gggaatgcgg
     3241 tcgccggcct tcaatggtcg cgccaggtcg atgaacatca catgcttgcc gcccggcttg
     3301 aagtcgacgg tctcgccggc cttgaaccgc acgccgtcct tcagttcctc catccgcatc
     3361 atgccgccgg ccgtcgaggt ctcgtgcatc tgcaccctcg cggcgaccgg ggattccacg
     3421 gcgatcagta cgtcaggacc gctgttgttg ttggtcaccg agaagaaccc ggctcccgtg
     3481 ccgccggcgg gggcaggccg gctgaacggt tcggcgaccg cgatgccggc gctgctgtag
     3541 gcgggcggtt tcgcgggcga gcaggcggcg agcagcaggg cggcgaggag aggcgcgagg
     3601 cgcatgatga agagcctttc gagctagaga ccgtgtttca gtttcagggt gtttgacgca
     3661 agcccatcga cggcgaaggc cagcagaatc gacaggccga gcagcggaag gaccagaccg
     3721 atcgccaggg cgaaaagcgc gaaggcgctc aagccccggg gctgggcggg cggagccgcc
     3781 aatcgtcccg caggccggcg acgccaccac atcaccgcgg ccgaaatgct cagggtgacg
     3841 atggccaggc atcccgccag catgaccagc tggttgaccc gcccgaactg gccgccggtg
     3901 tgcacggcga tgccccattc cacggccttc gacaccgggc cgtagtccct gaagccaagg
     3961 tcggcgatga cccgcccggt atactggtcg agatggatga ccctctggcc ggtcgcctga
     4021 cgcggatagc tctggatcgt gtagaccccg ccggccgagc ccggcaggcg caccacatag
     4081 ccgggagcga cgccgcgccg gctggcctcg gccatgatgg cggcgatcgg caacgggcgg
     4141 agggtggcgg ccgggacggt cgggacggcc gcccggtcca tggtccatga gccggccctg
     4201 gtcgccgcca gggccgcgtc ggacgccacc gtcgtcgcct tgagcccggc cggcatgccg
     4261 aggccggagt cattgaccag acgccggaaa ttctcgcccc agtagcctga ccagggcatg
     4321 ccggtgacgg ccaggaacag caggatggcg caggcgaagg cgccggtgac ggcgtgcagg
     4381 tcgcgccacc agacccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnccacag gaagacgccc gaggcgacca gcaggatggc ccagccggcg accacctcga
     4501 tgaccaggtt gggcgccttg ccgagaatct cgaggctgtg gatgtggctg atcagggtca
     4561 tcggccgctg gtcgtcggcg accctgtcga gcacccggcc tgtcgagggg acgaggaaga
     4621 ccgagacgac ctcgccccgg ccgtcgcgga tgccgacctc ggccgagcgg tccggcgccg
     4681 ggggcggggt gaagcgaatc ggcgttccgg gctgcgcggc cagggcccgg gcgatgagga
     4741 cctcgggcgg cagcgagggg gccgcggtcg cctcgacgac cagtcgccga tgttccagca
     4801 gcggcgccag gtcgtcgcgg aacaggtaga gcgcgccggt gctggcgagc aggatgaaga
     4861 acggcaggca gatcaggccg gcatagaaat gccagcgcca gatcgttcgg tagaggcggc
     4921 tcgcgcccgg cgggggctct tcggcggaca tgggaggatc tccggacggg cggcggcggg
     4981 tcgcgccgcc gccctctggg ctagaacgag accgtcaggc cggcgaacag cgacctgccg
     5041 tcgccgggcc agtaggccgc cgtggccggt ccggcgagga cagccgcctg gacgttggag
     5101 acgtaggcct cgtcagtcag gttgcgggcg tcgacgaaca gcgacaggcc gtccctcacg
     5161 gcccagcccg cgttcaggtt cgcgatggcg taggcggggg ccgtggtggt gttggcgtag
     5221 tcgacctcga tgtcggacgc cgaccattcg accgacgggg cgatgaacca gccggacggc
     5281 gcgtcgtagc gcagctcggc ccgatagaaa tgctcgggca cgatcggcag gcggttgtcg
     5341 ccgtactggc tgtcgccgtc gaagcggaag tccgaccagg tccaggtctg gcgcagccgc
     5401 caccgcggca ggaagcgcca gtccaccgcc gcctcgagcc cctggtggat cgtgcggtcg
     5461 gcgttgaagg tcgaggccgg gatgcccggc gcgaccgtgt actgcagcat ctccctgtca
     5521 agcaccgcgc ggtagagcgt gacgtcccag gtgaacgggc cgcgccggcc ccgcgcgccg
     5581 gcttccgccg tccaggcttc ctgggcctca accggggcga acccggttcc ggtgggcgac
     5641 atcgacccga aattcggcgg ctcgaccgat ttggtcaggt tggcgaacag ctgggcgccg
     5701 tcctcggcct gccacagcag gccgacgcgc ggcgcgaacc agtcgtagtc ccggctggcc
     5761 gtcaggttga aggttccggc gacgcccggc agggcgaaac tccggtagtc gcggccggcc
     5821 cggccccagg tcccgccacc gacgagggcg agatggtcgg agacgaacag ccggccctcg
     5881 gcgaacaggt cggcggcgtc ggcgttctgc agggtgcgtg aacggggcgc gccgcgatgg
     5941 cctttcacat tgacgtagaa gttcgagtcc aggtcgccag tgcgcagcca caggccggcc
     6001 caggcgtcag ccttcatccc ggcgatctcg ccctgccagt cgaggcgacc gaaggcgccc
     6061 cagttgcggc tctgctggtc gatgacctgg aagatcggat ggtccaggtc cttccacaga
     6121 ccatagaccg cgccctcgaa cagcgtgctg tcgccgagcc gccagcgggt gcgcagcgag
     6181 gcgcgcaggc cgcgctggtt gcggccctgg tcgttggcgt agttggccgg cgctgtctgg
     6241 ctcggatcgg cctggaactg ggaccgggtg agggcgccgg ggatttcctg gttgatgttc
     6301 gagccgttga cgatcagccg cacctcgcga tcctcgccga acgaccggcc gagattcagc
     6361 gaaccgaact ggatgttctg ctggctctgc gaccgccagc cctgggcggt ctggttcgtg
     6421 ccggagacgt agacatccca gtcgtctgac cgacgggcga gggcgatgtt ctcgcgcagc
     6481 aggccgtagg aaccaccgtc gatgcgcacc tgctgttcga agcccgccgt cgcgccggtg
     6541 ggggtgacca tgttgatcgc cccgccgagc agcgagccgc cgaagcgcag ggcgttgccg
     6601 ccgcgataga tctcgatgaa gcgcgtggac agaggatcgg cgatctggct gtcgccgtag
     6661 ccgtcggcct cgttcaacgg cacgccgtcc tgggccagca gcaggccgcg gttgtggttg
     6721 gcgttgccga tgcccgatcc gcggatcgag atgcgcacgt cgccgcccca cttgcgctgg
     6781 gcatagacgc ccggcgcgtc gcgcagcatg tcgtcgaagg cgagggactc gcggctggtg
     6841 aatgattccg ccgagatcac cgacaccgcg ccgggcgtgc gcgacaggcg tctgcgggcg
     6901 tcggcgacaa cggcggggtc ttccgggtcc ggattggcgg tgacgatgac gctgtcgacc
     6961 gtggtcggcg cgtcggtggc gtgggcgaga gcgggcgcgg cgagacagct cgcgccgagc
     7021 agcatggcgc gcaggcgcgc gccctggttc atggtcatgt gcggtgaatt ccagaagggt
     7081 ctgacagggc gcgtgtcggc gcgcccgcga aggggtcagg cgttctggag ggagggcggt
     7141 ccggtcgagt gtggccgggg cggcgcgcgg gcgccgggca ggcgatcggg cccgtcgacc
     7201 ggagcgaggc gcgattcggc cgggtagcga acgggcaggg cgagcgcctc gttcgccgag
     7261 aacaccaacg ggggcacccg caggccgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgcgg acgtcgcttt
     7381 tcgtttccgt ggcaagggat gccgccggga tcagcagctg gacgcagagt gcgagcaggg
     7441 ccagcgccgc ggtcagggcg ccgcggcgcg gggtcggccg gacggggcgg cgactgcgca
     7501 tgtcggcgga ctacgccgcc gcgcggggtt ccgcaagcgc cccgcaggtc ctcatatggc
     7561 accctcggca atctggaggc ggcatggcgg cggaagtctc tcccggcgag tacaaggacg
     7621 tggtcctgtt cctggcgacg gcgggcgtgg tcgtgcccct gttccgccgc tggcgggnnn
     7681 nnnnnnncga tcctggggtt tctcggcgcc ggggtggtgc tgggtccgtc cggcctgggg
     7741 gccctggccg gatcgctgcc ctggctcggc gccgtgacca tcgacaatcc gaaggacgtc
     7801 gcccagctgg ccgagttcgg ggtcgtgttc ctgctgttca tgatcgggct ggagctgagc
     7861 tgggagcggt tgcggctgat gcggcgcttc gtgttcggca tgggcgccct gcaggtggcc
     7921 ctttgttcgg ccgccatcgc caccatcgcc accctgctgg gccagccgct gcaggcggcc
     7981 gtggccatcg gcgcggcgct ggcgctgtcc tcgacggcca tggtcatgcc gctgctggcc
     8041 gagagcaagc gccagcacag ccaggcgggc cgtgcgacct tcgccgtgct gctgttccag
     8101 gacctggccg tggccccgat cctgatcacc ctgacggtga tcggcagcca gggcggcggc
     8161 gatctgttct cgccgaagat gctgctgacc ttcgcgccgg ccgccctggg cctgctggcc
     8221 ctggtggtgt tcggccggct gctgctgcga ccgatgatga agtcggtggc ccgggccagg
     8281 agcgaggagc tgttcatggc cgcctcgttg ctgatcgtca tcggcgcggg cctggtcgcg
     8341 gccctgaccg gcctgtcgat ggccctgggc gcgttcgtcg ccggcctgct gctggcggag
     8401 accgagtacc ggcacgaggt cgaggtgacc atcgagccgt tcaagggcct gttgctgggc
     8461 ctgttctttc tgtcgatcgg catcggtctc gacctgtcgc tgctgttcca gaagccggcg
     8521 ctgatcctcg gcatggcgct cggggcgctg atcctgaccg gcggggtggt gttcctcctc
     8581 gggctgctgt tcgggctcaa gccgcgcaag gcgcttgaag cgggcctgct gctggcggcg
     8641 ggaggcgagt tcgccttcgt gatcctcgac acggcgatga acagcggcct ggtcgaccgc
     8701 gaggtcggtc gcacggtgct ggtggcctcg accctgacca tgttctgcat cccggccctg
     8761 gcggccctcg gcgcgagact gggcggtcgt ccgggagcca atccggcgac cagcaccccg
     8821 gtcgagccgc ctctgtcgac cgacgagccg gccgtgctgg tggtcggcta tggccgggtc
     8881 ggccggctgg tcggcgacat gctcggccgc cacgacatcc gctgggtcgc cgccgagcgt
     8941 gatccgaagc tggtcgaggc cggacggcgc gccggggtgg cgatcttcta cggcgacgcc
     9001 tcgcgggccg agttcctgcg ccgctgcggc ctggacagcg ccccggccgt ggtcgtcacg
     9061 atggacaatc ccgagggcgc cgaggtggtc gtcgccacgg cgcgcgagct gcgtcccgac
     9121 ctgaccatcg tcgcccgggc ccgtgacgcc cgccacgcca agcggctcta cgaactgggc
     9181 gcgaccgacg cggtgcccga gaccatcgag gcttcgctgc agctgtcgga ggcggcgctg
     9241 gtcgacatcg gcgtgccgat gggcctggtc atcgcctcga tccatgagcg gcgcgacgag
     9301 ttccgcagcg agctcaaccg gcccgaagcc ctcggcggcc gacgccgcct gcgggacggg
     9361 acgaagccgt agatcggccc cccagagctg ccgaatgccg gcagatcacc gatcggtcgc
     9421 gatcagtccc agttcgcccc aacgcgcgta ctgcatggcc tccttccggg ctgccggcgt
     9481 cagtttttcc cagtccagat cgagcagcgc ctcggtgcat tcgatggcga tctgttcccg
     9541 ccgggtcgca cggcaggcct tcatcagcgt cgcgatgacc gccctgtctc cagcgcgggc
     9601 ggcgatgcgg acgtcctcgg gcagaaagta gtcgctgtcg cccgtccggg tgcgaccctc
     9661 tgcgatgatg gtccgggcct tcgtcacctg gccagagccc gtgtagagct tggccagttc
     9721 gcggtaggcc atcggcgaga tgttcggcgc cacgcgcgcc gcctccagat gggtgatggc
     9781 gccggccagg tcgccggagt caaccgccac gcgggccgcc cagaatcgga tataggcttc
     9841 gcgacggtag cgggtggcca tcgccttggt gatctcggtt cgggccgcgg cgacatcggc
     9901 gttggccagc gcatcctgac aggccgccgc cgcggcggcc gcgaccaggg cctccctgac
     9961 ttcggccgag cgcaccaggc tgtcgacctc ctcccggagg ggccggggtt ccggcgtcag
    10021 cccggccttg acgtaggcgg tctccttgta gcggtcgaga ccctgctcgc gggcctcagc
    10081 gctgcgatgg tcgcgcgtca ggtagtcgag cagccactgg cgagcgaagt cgtagagccc
    10141 cttcttgata ctttcgccga ccgcgccgaa ggcggcgccc gtgtcgcccg catcgacggc
    10201 cgactggaac ttggggtcgg acaccaggcc gcccacgcgt ttgtcgatcg atgcctgggc
    10261 cgccttgacc cgatcgctcc aagccttgtc ggacttctcg aagcgtatcg cggtgtaggc
    10321 gaactgggga tagtagccga gcgaggtgat gaggtccgtg ccggcggtgt cggcctcgtc
    10381 ctcattggta cgtccccagg cgggctcgac catcgacttg gtgatgaagc gcaggttgtc
    10441 atgggcgcgc tgggcgatgc cgatgcggtc gccgagctcc gggtcctgca cgttcgcctg
    10501 gctgtattgc aggctggacg tcagctgcgc cccgctcata taggccgtgg ccatgctgtt
    10561 gagcaggcga acctggccat agatggactc ggccttgtcc gtgtggccga gcgcgatgtg
    10621 cgcgtactca tgtgccagga gccaatagag gtcggtgtcg cgcaggccgc tttccgtctc
    10681 gtcctcaccg ggcgcgaaga ttcccaccat caccacgatc gtgttgtccg cggtggccat
    10741 ggcattgaac gccatgccag cgctgaccag caccctcggc ctgggcgcgt cggggtaggg
    10801 ccaggccgag gcgatccgat cgacgatccg ctgcaggtgc gcctgggtct gctccatacg
    10861 gaacaattcg ggccgcaaat cgcccttgac gtcggcgaag gcggccaggc cgagcgccgg
    10921 tcggaccgcc ccgatcgtgc tacccgcagg cgccgtcgcc gccattgccc gggcggccga
    10981 gcgggcggct acccgctcgc gcaccttggc gctgcagagg aacacgccat cctcgccggt
    11041 gatcgaaaac cggtcgccga cgatcgacag ggcccggccg ccagccccgc cgaacgcgcg
    11101 tctcgcgccg gccagcgtcc ccttcagccc acctggcttc ctgccgtcag t
//
LOCUS       DAORNX010000039        11126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4411_length_11126_cov_7.660193, whole genome shotgun sequence.
ACCESSION   DAORNX010000039 DAORNX010000000
VERSION     DAORNX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4411_length_11126_cov_7.660193"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLO65_02990"
     CDS             complement(<1..223)
                     /locus_tag="PLO65_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HRD27247.1"
                     /translation="MPATLRGAGRGNARPRAKAPVSPGKPRASAKGPQSAAKLRAARG
                     VGLPPRLAIAVAGLVFGVGVVATLATGNRA"
     gene            complement(189..1136)
                     /locus_tag="PLO65_02995"
     CDS             complement(189..1136)
                     /locus_tag="PLO65_02995"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRD27248.1"
                     /translation="MSLPLAGRHVAVLLGGLSSEREVSLVSGAACATALETLGARVTR
                     VDAGRDLAQVLTALKPDVCFNALHGAWGEDGCVQGVLETLAIPYTHCGVLASALAMDK
                     AKSKAVLAAAGVTVPGGGLYNRFDAAQRHVIPPPYVVKPNAEGSSVGVFLVFDGANQP
                     PQEIVAPSWTFGEEVMIEPYIDGLELAVAVRDGKALTVTEIVPRTGFYDYDAKYAEGG
                     SEHVVPARMPPEDFDRALRMAELAHAALGCRGVTRSDLRYDPVKRLLVLLEVNTQPGM
                     TPTSLVPEQAAHLGMAFDQLVLWIVEDAYARDIAGGGAR"
     gene            complement(1241..2638)
                     /locus_tag="PLO65_03000"
     CDS             complement(1241..2638)
                     /locus_tag="PLO65_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD27249.1"
                     /translation="MRRVILFTALTLLCTSACAADPAVAAPSADTAATLRDRALTDTT
                     AWTVLEDLTSEVGPRLVGSPGMARAKEWAVKTFTDMGFTNVKVEEFAKPSWIRGPESA
                     AIVGPYPFQLSIIGLGRTVPTPAGGIEAEVAVFPSYAALLAAPMGSLKGKIAVVTQPM
                     TRTQTGEGYGAAGIVRRSGASEAAKRGAVAFLMRSVSTSDSRLPHTGVLGYSDDAPKI
                     PAAALGVPDAELIERLAARGPVRIRMSLASTIDPDGVAWNISGEIRGSGKPEEVIVIG
                     GHLDSWDPGTGAIDDAAGIAITAAAAKLIGDQPKHPRRTIRVVMWGSEESGGSSEAYL
                     EAHRAELPNIVMASESDLGADRIYTLMLPKDALKAPALAGLSTVLAPLKIFVSRDPAP
                     FGGSDVEGLQEAGVPVFTLRQDASRYFDLHHSADDTIDKVDPAQLNQNVAAWAALLWM
                     LADTDTDFRAPTAAP"
     gene            complement(2643..3557)
                     /gene="murB"
                     /locus_tag="PLO65_03005"
     CDS             complement(2643..3557)
                     /gene="murB"
                     /locus_tag="PLO65_03005"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRD27250.1"
                     /translation="MTWRDALPTVRGKLLFDEPLGPFTWFRVGGPADVVFLPEDEADL
                     AGFLKALDPAAPVMAIGVGSNLLVRDGGVEGVVIRLGRGFNTVETLGDSRIRAGSAVP
                     DAILARKAAEAGIAGLEFYAGIPGTVGGAVIMNAGCYGAETVNVLESARVMDRTGAVR
                     DMPLAQLGYTYRHSAIQDGDLVVLGATFRGTPDGPAAIKARMAEITARRETTQPIREK
                     TGGSTFKNPPGHSSWKLVDEAGWRGKLFGGAMFSPLHSNFMINTGEATAADLEGLGEA
                     VRAAVMDRTGVSLEWEIKRVGKPLPSKE"
     gene            complement(3682..5100)
                     /gene="murC"
                     /locus_tag="PLO65_03010"
     CDS             complement(3682..5100)
                     /gene="murC"
                     /locus_tag="PLO65_03010"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRD27251.1"
                     /translation="MIQRRRPVPFEIGPVHFIGIGGIGMSGIAEIMIRIGYTVQGSDL
                     KPSANTDRLEKLGARIFIGHEGGQVEGASAVVYSTAVKPDNPEMVAARERRLPLVRRA
                     EMLAELMRLQFSIAVGGTHGKTTTTSMIATLLDAGGLDPTVVNGGIINAYGTNAKVGE
                     GDWIVVEADESDGTFLKLKSTVAVVTNIDAEHLDHWGDFDAVRKGFQDFVENIPFYGF
                     AAICVDHPEVQALSARVENRRLVTYGTNPQAEVRATHIGMGPDGAKFDVQISPRDGEP
                     IRYEGLTLPMAGLHNVMNACAAIAVARELGVDAAAIRAGLAAFGGVKRRFTTTGVVKG
                     VRIIDDYAHHPVEIAAVLTAARAVTGEGKVIAVVQPHRFTRLRDLMAEFSACFNDADV
                     VIVADVYTAGETPIDGVDRDALVEGLRRYGHRRAIPLEGPAALAAMVRDEAKAGDIVV
                     LLGAGDITSWAYALPGQLEALA"
     gene            complement(5110..6207)
                     /gene="murG"
                     /locus_tag="PLO65_03015"
     CDS             complement(5110..6207)
                     /gene="murG"
                     /locus_tag="PLO65_03015"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRD27252.1"
                     /translation="MPERIAVVAAGGTGGHLFPAQALAEALIRRGWRILLATDERGAL
                     YADKFPADLRISLSAATAKSGDPLGMVRAGFAVLRGTMQARAIFRRMDPAVVVGFGGY
                     PSLPALLAAISQKRPTLIHEQNAVLGRVNRFLAPRVDQVACAFPTLEMADPRVKGRAH
                     VVGNPVRPEIRALFDQPYHPPEPGGPIRLLITGGSQGARLLSELTPEAILRLPEDLRA
                     RLQVQQQTRKESMDGARRVYANALVKAEVAPFFRDMAGRLAAAHLVIGRAGASTVCEL
                     AVAGRPSLLVPLKIAADDHQRYNARLLSDADAAAVALEDELTVDSLAGALNALLRDPA
                     WLSRMAAGARSVARPDAAEKLADLVERTALG"
     gene            complement(6217..7386)
                     /gene="ftsW"
                     /locus_tag="PLO65_03020"
     CDS             complement(6217..7386)
                     /gene="ftsW"
                     /locus_tag="PLO65_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRD27253.1"
                     /translation="MSYSPHTFLRSDTTAVGNWWWTMDRWLLGAVFTLITLGVLLSFG
                     TSPAAAARLHYDDAFHFAVRQSLFGVLAAGLVIGVSMLEARTIRRVAFFVYVAMIVVM
                     AALPFIGHEAKGATRWIHFAGFTLQPSEFMKPALIVLVAWMFAEGQKGEGVPGVFIAF
                     CLYLLAVGLLLKQPDLGQTILITVAFGAVFWMAGVPISWIMGLGVVAISGLCSTYFLF
                     PHVQARVETFLSSDKADKHQITQASEAIWAGGLFGRGPGEGVMKRGVPDMHTDFAYSV
                     LAEEYGLVFSLALIGLFAFVVVRGLYKSLRLSDPFEQVAAAGLFVLVGLQALINVAVN
                     LNMIPTKGMTLPFISYGGSSMLAMGLTLGMALGLTRRRPGAYAPSEGLARAGAFA"
     gene            complement(7383..8798)
                     /gene="murD"
                     /locus_tag="PLO65_03025"
     CDS             complement(7383..8798)
                     /gene="murD"
                     /locus_tag="PLO65_03025"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRD27254.1"
                     /translation="MIPIRGFEGRKVAVFGLGRTGLTAARALVAGGAEVAVWDEKEAG
                     REAARAEGFTPVDLTAADWSGFAALMLSPGVPLTHPRPHWTVEKAKTAGVEVLGDIEL
                     FARTVNAAPPHKRPKVAAITGTNGKSTTTALLGHLCAGAGRDTRIGGNIGVGVLGLPD
                     MHGGAVYVLEVSSYQLDLTASLKPDAAVLLNISPDHLDRHGSMDGYIAAKRRVLLNQT
                     KGDTAIIGVDDAWCQRICTEITAANHRTIWPISASRSMGRGVYAIQGVLYDATGDRVV
                     EVADLLRARSLPGRHNWQNAAAAYAAARALGVPAEEAAEGLMTFPGLAHRMETVATLG
                     RVRFVNDSKATNADAARQALSSYPRVYWIAGGQPKSDGIAPLSDLFGRVAKAYLIGEG
                     AGAFARVLEGRAPHVVSGTIEAATAAAYADADASGEDAIVLLSPACASFDQFSDFEAR
                     GEAFRAAVESLQRPALKGARA"
     gene            complement(8817..9917)
                     /gene="mraY"
                     /locus_tag="PLO65_03030"
     CDS             complement(8817..9917)
                     /gene="mraY"
                     /locus_tag="PLO65_03030"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRD27255.1"
                     /translation="MLQFLADWRDSVPLLNLLKYLTFRTGMATATGFIIAVAMGSRFI
                     NWMRAKQGKGQPIRKEGIERHVLEKAGTPTMGGLMILAGLIGGTLLWANLTNIYVWVV
                     LMVTAGYGVLGFMDDYDKVTKQSTAGVSGPVKLIIQFAIAFAAVALLIWFGGKSPDAP
                     ELSTSVAFPIFKRLLLDLGWFYLAFAAVVIVGFSNAVNFTDGLDGLAIVPVMIAAAAL
                     GLIAYLVGNYVFAEYLQVHFVPGVGELAVICGALIGAGLGFLWYNAPPAKIFMGDTGS
                     LALGGALGVISVATKHEIVLAIIGGLFVAELLSVIIQVVYFKRTGRRIFLMAPIHHHF
                     EKLGWPESTVVIRFWIVSLMMAFIGLATLKLR"
     gene            complement(9917..>11126)
                     /locus_tag="PLO65_03035"
     CDS             complement(9917..>11126)
                     /locus_tag="PLO65_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRD27256.1"
                     /translation="GAAGVLASRPVEGPHVLVSDTLRALEAMAVAARDRSLAVRAAVT
                     GSVGKTSVTQAIAAGLAGAGRWHASVKSYNNHIGVPLTLARMPWATERAVFEIGMNHA
                     DEISPLSRMVRPDVAVITTVGAVHIENFPDGEEGVARAKAEIFDGMDSTGVAILNADN
                     RWFDLLRHHARAVGVRVLSFGSAAGCDARLLDFSAAHGHASVRAEVHGSAISYPLLQT
                     GLHWGLNSLCALLALEAMSVDRATALEALAAFAPLAGRGAERTVRIPGGAFTLIDESY
                     NANPISMAAALKTLGARQGRGRRIVALTDMLELGAEAPAFHAALAGPIEAAGIDLVFC
                     AGPMMKSLWDALPPTRRGGYAEAAADLAPRVASAAEPGDLVMVKGSNGSRASVIAAAL
                     AALDRTGEMA"
BASE COUNT         1755 a         3851 c         3925 g         1595 t
ORIGIN      
        1 cggcgcggtt gccggtcgcc agggtggcga ccacgccgac gccgaacacc agcccggcca
       61 cggcgatggc cagcctgggc ggcaggccga cgccacgcgc agcgcgcagc ttggccgcgg
      121 actggggccc ctttgcggag gcgcggggtt ttcccggact gacgggcgct ttggccctgg
      181 gcctggcgtt acctcgcccc gccccccgca atgtcgcggg catacgcgtc ctccacgatc
      241 cagagcacca gttgatcgaa cgccattccc agatgggccg cctgctcggg aaccagagaa
      301 gtcggcgtca ttccgggttg cgtgttgacc tccaacagga ccagaagacg cttaacgggg
      361 tcataacgaa gatcacttcg ggtcacaccc cggcaaccaa gggcggcgtg ggcgagctcg
      421 gccatgcgca gggcccggtc gaagtcctcg ggcggcatgc gggccggcac cacatgctcc
      481 gacccgccct cggcgtactt ggcgtcgtaa tcgtagaatc cggtgcgcgg gacgatctcg
      541 gtgaccgtca gggccttgcc gtcgcggacc gcgaccgcca gttcgagccc gtcgatatag
      601 ggctcgatca tcacctcctc gccgaaggtc cagcttgggg cgacgatttc ctgtggcggc
      661 tggttggccc cgtcgaagac caggaacacg ccgacagatg agccctcggc gttgggtttg
      721 accacatagg gcggcgggat gacgtgacgc tgtgccgcat cgaagcggtt gtacaaaccc
      781 ccgcccggca ccgtcacgcc ggcggcggcg agcaccgcct tcgacttggc cttgtccatg
      841 gccagcgcgg acgccagcac gccgcagtgg gtgtagggta tggcgagggt ctccagcacg
      901 ccctggacgc agccgtcctc gccccaggcg ccgtgcaggg cgttgaagca gacgtcgggt
      961 ttcagcgccg tcagcacctg ggccaggtcg cgacctgcat cgacgcgggt gaccctggcc
     1021 cccagggtct ccagcgcggt ggcgcaggcg gcgccggaca cgaggctgac ctcgcgctcg
     1081 gaggacaggc cgccgagcag aacggccacg tggcggccag ccaggggaag ggacattcag
     1141 ggctccgaaa tgggtcgcag tgctttagcc gcgatagcgg ccggttgtta agccggcgcc
     1201 gccatcgcgt ggagtttccg tccagaaccc cggacggggt ctagggcgcg gcggtcggcg
     1261 cgcggaaatc ggtgtcggtg tcggccagca tccacagcag cgccgcccag gcggcgacgt
     1321 tctggttcag ctgggccgga tcgaccttgt cgatcgtgtc gtcggccgaa tggtgcaggt
     1381 cgaaatagcg gctggcgtcc tggcgcaggg tgaagaccgg aacaccggcc tcctgcaggc
     1441 cttccacgtc cgaaccgccg aacggcgccg ggtcgcgcga aacgaagatc ttgagcggtg
     1501 ccagaaccgt cgacaggccg gccagggccg gcgccttgag ggcgtccttg ggcagcatca
     1561 gggtgtagat ccgatcggcg ccaaggtcgc tctcgctggc catgacgatg ttgggcagtt
     1621 cggccctgtg cgcctcgaga taggcttcgc tggacccgcc cgactcctcc gagccccaca
     1681 tgaccacgcg gatcgtgcgc cgcgggtgtt tcggctggtc gccgatcagt ttggccgcgg
     1741 cggccgtgat ggcgatcccg gccgcgtcgt cgatggcgcc cgtgccgggg tcccagctgt
     1801 cgagatggcc gccgatgacg atcacctcct ccggcttgcc gctgccgcga atctcgccgg
     1861 agatgttcca ggcgaccccg tctggatcga tggtcgaggc cagactcatg cgaatccgca
     1921 ccggaccgcg ggcggccagc cgctcgatca gctcggcgtc gggaacgcca agggccgcgg
     1981 ccggaatctt cggcgcgtca tcgctgtagc ccagcacgcc cgtatgcggc aggcgcgagt
     2041 ccgaggtcga gaccgaacgc atcaggaagg ccaccgcccc gcgtttggcc gcctccgacg
     2101 cgcccgaacg ccggacgatg cctgcggccc cgtagccctc gccggtctgg gttcgggtca
     2161 tcggttgggt gaccacggcg atcttgccct tcagcgagcc catcggcgcg gccagcaggg
     2221 cggcgtagga ggggaacacc gcgacctcgg cctcgatgcc cccggccggc gtgggaaccg
     2281 tgcggccgag accgatgatc gaaagctgga acgggtaggg accgacgatc gcggccgact
     2341 cggggccccg gatccagctc ggtttggcga actcctcgac cttcacattg gtgaagccca
     2401 tgtcggtgaa ggtcttcacc gcccactcct tcgcccgggc catgcccgga gagccgacca
     2461 ggcgcggccc cacctcgctg gtcaggtcct ccagcaccgt ccaggcggtg gtgtcggtca
     2521 gggcgcgatc cctcagcgtc gccgcggtgt cggcggacgg cgcggccacg gcggggtcgg
     2581 ccgcgcaggc cgaggtgcac agcagggtca gggcggtgaa caggatgacg cggcgcattg
     2641 gtctactcct tcgacggcag gggtttgccg acgcgtttga tttcccactc cagcgagacg
     2701 ccggtccggt ccatgacagc cgcgcggaca gcctcgccga ggccttccag atccgcggcc
     2761 gtggcctcgc cggtgttgat catgaagttg ctgtgcaggg gcgagaacat cgccccgcca
     2821 aacagctttc cacgccagcc ggcctcgtcg accagcttcc aggacgaatg gccgggcggg
     2881 ttcttgaagg tcgagccgcc ggtcttctcg cggatcggct gggtggtttc acggcgggcg
     2941 gtgatctcgg ccatgcgggc cttgatcgcg gcgggcccgt cgggggtccc gcgaaaggtc
     3001 gcgccgagca cgacgaggtc gccgtcctgg atcgcgctgt ggcggtaggt gtagccaagc
     3061 tgggccagcg gcatgtcacg gaccgcgccc gtgcggtcca tcacccgggc agactcgagg
     3121 acgttgaccg tctcggcgcc gtagcagccg gcattcatga tcaccgcccc gcccaccgtc
     3181 ccggggatgc cggcgtagaa ctccagcccg gcgatgcccg cctcggccgc cttgcgggcg
     3241 agaatggcgt ccggaacggc cgagccggcg cgaatccggc tgtcgccgag cgtctcgacg
     3301 gtgttgaagc cccggcccag ccggatgacc acgccctcga ccccgccgtc gcgcaccagc
     3361 aggttggagc cgactccgat ggccatcacg ggcgccgccg ggtcgagggc cttcaggaaa
     3421 ccagcgaggt cggcctcgtc ctccggcagg aacaccacgt ccgccggccc gccgacgcgg
     3481 aaccaggtga acggccccag cggctcgtcg aacaacagct tgccgcggac cgtcggaagc
     3541 gcgtcgcgcc aggtcatggg cgcgttcctg cgtggttcga cacgcgcttc gcgctgctca
     3601 ccatgacgtg aaggggcgca ccgtcacagg cgcacgtcat cctgagcagg cccgcagggc
     3661 cgtgtcgaag gacgcacgac ctcacgccag cgcctccagc tgacccggca aggcgtaggc
     3721 ccagctggtg atgtcgcccg cgccgagcag gacgacgata tcgccggcct tcgcctcgtc
     3781 cctgaccatc gcggccaggg ccgcgggccc ttcgagcggg atcgcgcggc ggtggccgta
     3841 gcgacgaagg ccctcgacca gcgcgtcgcg gtcgacaccg tcgatcggcg tctcgccggc
     3901 ggtgtagacg tcggccacga tgaccacgtc ggcgtcgttg aagcaggcgc tgaactcggc
     3961 catcaggtcg cgcaggcgcg tgaaccggtg cggctggacc acggcgatga ccttgccctc
     4021 gccggtcacg gcgcgggcgg cggtcagcac ggcggcgatc tccaccgggt ggtgggcgta
     4081 gtcgtcgatg atccgcacac ccttgaccac gccagtggtg gtgaagcggc gcttgacccc
     4141 gccgaaggcc gcaagccccg cgcggatcgc cgcggcgtcg actccgagct cccgggccac
     4201 ggcgatggcg gcgcaggcgt tcatgacatt gtgcaacccg gccatcggca gggtcaggcc
     4261 ctcgtagcgg atcggctcgc cgtcgcgcgg gctgatctgc acgtcgaact tcgcgccgtc
     4321 cggccccatg ccgatgtgcg ttgcccgaac ctcggcctgg gggttcgtcc cataggtcac
     4381 cagccgccgg ttctcgaccc gcgcgctgag cgcctggacc tccgggtgat ccacgcagat
     4441 ggccgcgaag ccgtagaacg ggatgttctc gacgaagtcc tggaagccct tcctgaccgc
     4501 gtcgaagtca ccccagtggt cgaggtgctc ggcgtcgatg ttggtcacca ccgcgacagt
     4561 cgacttgagc ttcaggaagg tgccgtcgct ctcgtcggcc tcgacgacga tccagtcgcc
     4621 ctcgccgacc ttggcgttgg tgccgtaggc gttgatgatc ccgccgttga ccaccgtggg
     4681 gtccagcccg ccggcgtcga gcagggtggc gatcatcgag gtggtggtgg tcttgccatg
     4741 ggtgccgccg acggcgatcg agaactgcag ccgcatcagc tcggccagca tctcggcgcg
     4801 gcggacgagc ggcagccggc gctcgcgggc cgcgaccatc tcggggttgt cgggtttcac
     4861 ggccgtcgaa tagaccacgg ccgaggcgcc ctcgacctga ccgccctcat ggccgatgaa
     4921 gatgcgcgcg cccagctttt ccagccggtc ggtgttggcg ctcggtttca ggtccgagcc
     4981 ctgcaccgtg tagccgatac ggatcatgat ctcggcgatg ccgctcatgc cgatgccgcc
     5041 gatgccgatg aagtgcacgg ggccgatttc gaacggaacg gggcgacgac gctggatcat
     5101 tcgcgcggct taccccagcg cggtccgctc gaccagatcg gcgagttttt ccgcggcgtc
     5161 gggccgggcc acggaacggg cgccggcggc catgcgcgac agccatgccg gatcgcgcaa
     5221 caaggcgttc agcgccccgg ccaggctgtc gacggtcagc tcgtcctcca gcgccacggc
     5281 cgcggcgtcg gcgtcggaca gcagccgggc attgtagcgc tggtggtcgt cggcggcgat
     5341 cttcagcggc accagcagcg atggcctgcc ggccacggcc agttcgcaga ccgtcgaggc
     5401 gccggcccgg ccgatgacca ggtgggcggc ggccagccgt ccggccatgt cgcggaagaa
     5461 cggcgcgacc tcggccttga ccagggcgtt ggcgtagacc cgccgggcgc cgtccatcga
     5521 ctccttgcgc gtctgctgct gcacctgcag ccgggcgcgc aggtcctcgg gcaggcgcag
     5581 gatcgcctcg ggcgtcagct ccgacagcag gcgcgcgccc tggctgccgc cggtgatcag
     5641 caggcggatc ggtccgcccg gctcgggcgg atggtagggc tggtcgaaca gggcgcggat
     5701 ttcgggccgc accgggttac cgacgacatg agcgcggccc ttcacccggg gatcagccat
     5761 ctcgagggtc ggaaaggcgc aggccacctg gtcgacccgt ggggcgagga agcggttgac
     5821 ccgcccgagc accgcgttct gttcatggat cagggtcggg cgcttctggc tgatcgcggc
     5881 cagcagcgcc ggcagcgaag ggtagccgcc gaacccgacc accaccgccg ggtccatgcg
     5941 ccggaagatc gcgcgcgcct gcatcgtgcc gcgcaggacg gcgaagccgg cccggaccat
     6001 gcccagcgga tcgccggact tcgcggtcgc ggccgacagg ctgatccgca gatcggcggg
     6061 gaacttgtcg gcgtacagcg cgccgcgctc gtcagtggcc agcagaatcc gccagccacg
     6121 gcggatcagg gcctcggcca gcgcctgggc gggaaacagg tggccgcccg tgcctcctgc
     6181 ggcgacaaca gcaattcttt ccggcatgcg agcgtcttac gcgaaggcgc cggcgcgggc
     6241 gagaccctcg ctgggcgcat aggccccggg acgccgtcgg gtgagaccca gggccatgcc
     6301 cagcgtcagg cccatggcca gcatcgacga gccgccatag ctgatgaacg gcagggtcat
     6361 gcccttggtc gggatcatgt tgagattcac cgccacattg atcagagcct gcaggcccac
     6421 cagcacgaac aggccggcgg cggccacctg ctcgaacgga tccgacaggc gcagcgactt
     6481 gtacagcccg cgcacgacca cgaaggcgaa caggccgatc agggccagcg agaagaccaa
     6541 gccgtactcc tcggccagca ccgaataggc gaagtcggtg tgcatgtcgg gcacgccgcg
     6601 cttcatcacc ccctcgcccg gtccgcgacc gaacaggccg ccggcccaga tcgcctccga
     6661 ggcctgggtg atctggtgct tgtcggcctt gtcggaactg aggaaggtct cgacccgcgc
     6721 ctggacgtgc gggaacagga agtaggtgct gcagaggccg gagatggcga caacgcccaa
     6781 ccccatgatc cagctgatgg gcaccccagc catccagaac accgccccga aggcgacggt
     6841 gatcaggatg gtctggccaa ggtccggctg cttgagcagc aggccgaccg ccagcagata
     6901 gaggcagaag gcgatgaaca cgccgggcac gccctcgcct ttctggccct ccgcgaacat
     6961 ccaggcgacc agcacgatca gcgccggctt catgaattcg gagggctgca gggtgaagcc
     7021 cgcgaaatgg atccagcggg tcgcgccctt ggcctcgtgg ccaatgaagg gcagcgccgc
     7081 catgacgacg atcatcgcga catagacgaa gaaggccacc cggcggatgg tcctcgcctc
     7141 gagcatggag acgccgatca ccaggccggc ggcgagcacg ccgaacagcg actggcgcac
     7201 ggcgaaatgg aaggcgtcgt catagtgcag gcgcgcggcc gcggccgggc tggtgccgaa
     7261 cgacagcagc acgccaaggg tgatcagcgt gaacaccgcc ccgagcagcc agcggtccat
     7321 ggtccaccac cagttgccaa ccgccgtggt gtcggagcgc aggaaggtgt gggggctgta
     7381 gctcacgcgc gcgctccctt cagggccggc cgctgaaggg actcgaccgc ggcgcggaac
     7441 gcttcgccgc gcgcctcgaa atcgctgaac tggtcgaagg atgcgcaggc cggcgagagc
     7501 agcacgatgg cgtcctcgcc gctggcgtcg gcgtctgcgt aggccgcggc ggtagccgcc
     7561 tcgatcgtgc cggacacgac atggggcgcc cggccctcaa gcacccgggc gaaggcgcca
     7621 gcgccctcgc cgatcagata ggccttggcc acccggccaa acagatcgga cagcggggcg
     7681 atgccgtccg acttgggctg gccgccggcg atccagtaga cccgcggata gctgctgagc
     7741 gcctggcggg cggcgtcggc gttggtcgcc ttggagtcgt tgacgaagcg cacccggccc
     7801 agggtcgcca ccgtctccat ccggtgggcc agacccggga aggtcatcag gccctcggcc
     7861 gcctcctccg ccggcacccc cagcgcccgc gcggcggcgt aggcggccgc tgcgttctgc
     7921 cagttgtggc ggcccggcag ggaccgcgcg cgcaacaggt cggcgacctc gacgacccgg
     7981 tcgccggtgg cgtcatacag cacgccctgg atggcgtaga cgccgcgccc catcgagcga
     8041 ctggcgctga tcggccagat ggtccggtgg ttggcggcgg tgatctcggt gcagatgcgc
     8101 tggcaccagg cgtcatcgac gccgatgatg gccgtatcgc ccttggtctg gttgagcagc
     8161 acccgccgct tcgccgcgat atagccgtcc atgctgccat gccggtcgag gtgatcgggc
     8221 gagatgttga gcaggaccgc ggcgtcaggc ttgaggctgg cggtcaggtc gagctggtag
     8281 gacgacacct cgagcacata gaccgctccg ccatgcatgt ccgggaggcc cagcacgccg
     8341 acgccgatgt tgccgccgat gcgcgtatcg cggccggcgc cggcgcagag atgacccagc
     8401 agggccgtgg tggtcgactt gccgttggtg ccggtgatcg ccgccacctt cggacgtttg
     8461 tgcggcggag cggcgttcac cgtccgggcg aacagttcga tatcgccgag cacctcgacg
     8521 ccggcggtct tcgccttctc gaccgtccag tgcggccggg ggtgggtcag ggggacgccc
     8581 ggcgacagca tcagggcggc gaagccggac cagtcggcgg cggtcaggtc gaccggcgtg
     8641 aatccctcgg cgcgcgccgc ctcgcggccg gcttcctttt cgtcccacac agcgacctcc
     8701 gcgccgccgg cgacgagcgc gcgcgcggcc gtcagaccgg ttcggccgag accgaacacg
     8761 gcgaccttgc gtccctcgaa gccgcggatg gggatcatgg cgggtctctg cactccctag
     8821 cgaagcttga gggtggcgag gccaatgaag gccatcatca gcgagacgat ccagaagcgg
     8881 atgaccaccg tcgactccgg ccagcccagc ttctcgaagt ggtggtggat cggcgccatc
     8941 aggaagatac gcctgccggt gcgcttgaag tagacgacct ggatgatgac gctgagcagt
     9001 tcagcgacga acagcccgcc gatgatcgcc agcacgatct cgtgcttggt cgccaccgaa
     9061 atgacgccca gggccccgcc gagggccagc gagccggtgt cgcccatgaa gatcttggcc
     9121 ggcggcgcgt tgtaccagag gaagcccagg ccggcgccta tgagcgctcc gcagatcacc
     9181 gccagctcgc ccacgcccgg cacgaagtgg acctgcaggt attcggcgaa cacgtagttg
     9241 ccgaccagat aggcgatcag tccgagggcc gcggcggcga tcatcaccgg cacgatggcc
     9301 aggccatcga ggccgtcggt gaagttcacc gcattggaga agccgacgat cacgaccgcg
     9361 gcgaaggcca ggtagaacca gcccagatcc agcagcagcc gcttgaagat cgggaaggcc
     9421 accgaggtcg acagctccgg cgcgtccggc gacttgccgc cgaaccagat cagcagcgcg
     9481 acggcggcga aagcgatagc gaactggatg atcagcttga ccgggccgga caccccggcg
     9541 gtgctctgct tggtcacctt gtcgtagtcg tccatgaagc cgagcacgcc gtagccggcg
     9601 gtgaccatca gcacgaccca gacatagatg ttggtcagat tggcccacag cagggtgccg
     9661 ccgatcagcc cggccaggat catcagcccg cccatggtcg gggtgccggc cttttccagc
     9721 acgtggcgct cgatgccttc cttgcggatc ggctggccct tgccctgttt ggcgcgcatc
     9781 cagttgatga agcgcgagcc catcgccacg gcgatgatga aacccgtcgc cgtcgccatg
     9841 ccggttctga acgtcaggta tttcagcagg ttgagcagtg ggaccgagtc ccgccagtcc
     9901 gccaggaatt gcagcatcag gccatctccc ccgtccggtc gagcgcggcc agggccgcgg
     9961 cgatgacgct cgcccggctg ccgttcgacc ccttgaccat caccagatcg cccggctctg
    10021 cggccgatgc gacgcgcggc gccagatcgg ccgccgcttc ggcgtagccg ccgcgacgag
    10081 tcggcggaag ggcgtcccac agcgatttca tcatcggccc ggcgcaaaac accaggtcaa
    10141 tgccggccgc ctcgatcggc ccggcgaggg ccgcgtggaa cgcgggcgcc tcggccccca
    10201 gttcgagcat gtccgtcagg gccacgatcc gccggcctct gccctgccgg gcgccgagcg
    10261 tcttcagcgc cgcggccatt gagatcggat tggcgttgta gctctcgtca atcagggtga
    10321 aggccccgcc tgggatgcgc acggtccgtt cggccccacg cccggccagg ggcgcgaagg
    10381 ccgccagcgc ctccagggcc gtggcgcggt ccacgctcat cgcctcgagg gccaacagcg
    10441 cgcagaggct gttgagaccc cagtgcaggc cggtctgcaa cagcggatag ctgatcgccg
    10501 acccgtggac ctcggcgcga acgctggcgt ggccgtgggc ggcggagaag tcgagcagcc
    10561 gcgcgtcgca gcccgccgcc gagccgaacg acagcacacg gacgccgacg gccctggcgt
    10621 gatggcggag caggtcgaac cagcggttgt cggcgttgag aatggcgact cccgtcgagt
    10681 ccatgccgtc gaagatctcg gccttggccc gggcgacgcc ctcctcgccg tcgggaaagt
    10741 tctcgatgtg cacggcgccc acggtcgtga tcaccgcgac gtcggggcgc accatccgcg
    10801 agaggggcga aatctcgtcg gcgtggttca tgccgatctc gaacaccgcc cgttccgtgg
    10861 cccagggcat gcgggccagg gtcagtggca cgccgatgtg gttgttgtag gacttgaccg
    10921 aggcgtgcca acggcccgcg cccgccaggc cggcggcgat ggcctgggtg acgctggtct
    10981 tgcccaccga tccggtgacg gccgcgcgaa cggccagcga ccggtcacgg gcggcgacgg
    11041 ccatggcctc aagggcccga agcgtatcgg acaccaggac gtgcggcccc tcgaccgggc
    11101 gcgaggcgag cacgccggcg gccccg
//
LOCUS       DAORNX010000040        10975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4482_length_10975_cov_8.874908, whole genome shotgun sequence.
ACCESSION   DAORNX010000040 DAORNX010000000
VERSION     DAORNX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4482_length_10975_cov_8.874908"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLO65_03040"
     CDS             complement(<1..189)
                     /locus_tag="PLO65_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27257.1"
                     /translation="MSKPSATLRSDPLIPYVLAVFACCLAGALGGYGLARPREALAAR
                     GLAAAEAGGGGLGAARGFG"
     gene            complement(158..253)
                     /locus_tag="PLO65_03045"
                     /pseudo
     CDS             complement(158..253)
                     /locus_tag="PLO65_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            492..782
                     /gene="groES"
                     /locus_tag="PLO65_03050"
     CDS             492..782
                     /gene="groES"
                     /locus_tag="PLO65_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRD27258.1"
                     /translation="MKFRPLGDRVLVKRVEEEEKTKGGIIIPDTAKEKPQEGEVIAVG
                     PGARDDSGKVHALDVKAGDRILFGKWSGTEVKVNGEDLLIMKESDVLGVVEK"
     gene            838..2490
                     /gene="groL"
                     /locus_tag="PLO65_03055"
     CDS             838..2490
                     /gene="groL"
                     /locus_tag="PLO65_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516094.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRD27259.1"
                     /translation="MAAKDVYFSSDARDRMLRGVNILANAVKVTLGPKGRNVVIEKSF
                     GAPRSTKDGVSVAKEIELSDRFENLGAQLIREVASKTNDKAGDGTTTATVLAQAIVVE
                     GLKSVAAGMNPMDLKRGVDKAVTKVVEEIKNSSKKVTTNQEIAQVGAISANGDVEIGD
                     MIAKAMDKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFITNADKMETQLEEP
                     LILLFEKKLSGLQPLLPVLEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLRVAAV
                     KAPGFGDRRKAMLEDLAILTGGQVISEDLGIKLENVTLDMLGKAKKVTITKDDTTIVD
                     GSGDKDGIEARIAQIKRQIEDTTSDYDKEKLQERLAKLAGGVAVVRVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGVCLLKASKALESLTGDNADQTAGIAIVRRALQAP
                     IRQIAENAGVEGSIVVGKVLDGKDASFGFNAQTEEYGDLVKMGVIDPAKVVRTALQDA
                     ASVAGLLITTEAAIVEAPKKASAGAAGGMPGGGMGGMGDMDF"
     gene            complement(2578..2940)
                     /locus_tag="PLO65_03060"
     CDS             complement(2578..2940)
                     /locus_tag="PLO65_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRD27260.1"
                     /translation="MLLVRLALRTLFGMAGLWLAQKFVPGIHFADTQSLVIAALVLGV
                     VNAIVRPIVIFFTLPLTLVTLGLFLLVVNAGMIALTAWLMDGFTVAGFWPALFAAVVV
                     GVTSWFGHMLIGPREEGE"
     gene            complement(2987..4030)
                     /locus_tag="PLO65_03065"
     CDS             complement(2987..4030)
                     /locus_tag="PLO65_03065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRD27261.1"
                     /translation="MTASSKLTLPGLLTPSAAYKPFRYPWAYDMWKRQQQVHWMPEEV
                     PLGEDCKDWAANLNDRERNLLTQIFRFFTQGDIEVQDNYMDRYGRVFRPTEVKMMLSS
                     FANMETIHIAAYALLLETIGMPESEFGAFMEYEAMKAKHDYMQQFGVENNADIARTLA
                     MFGGFTEGLQLFASFAMLMNFPRFNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFNKE
                     TGAVTKSVADDIVECCRHVVHLEDRFIDLAFEAGDVQGMNPEDIKQYIRFIADWRLRQ
                     LKLPELYGVTENPLPWLQSLLSGVEHANFFEARSTEYSKAATRGQWHGEAGVWGEFDK
                     LMARRNENLLPAD"
     gene            4168..4830
                     /locus_tag="PLO65_03070"
     CDS             4168..4830
                     /locus_tag="PLO65_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27262.1"
                     /translation="MNQSLIVYGALGSGSVPVEAALTLLDLPFEVVEAATWEQDPQVL
                     ARVEAVNPLLQVPALVLPSGEVLTESAAILLWLADAHPEAGLGPGVDDPRRAQFLRWM
                     TFIPASIYSMFWVRDVPARLAGPDPVQGERVKQATAERIADCWRLMDRQITPAGAYLL
                     GDTLSVLDLYVAVVSRWGPGRNRFYREAPGMTPVVRRVDADPRLADFWARRFPFAAGW
                     ER"
     gene            complement(4827..5534)
                     /locus_tag="PLO65_03075"
     CDS             complement(4827..5534)
                     /locus_tag="PLO65_03075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD27263.1"
                     /translation="MAHNLLLATFGAHKPSRDGGAEMWLQEANHRIANNLSLVAGLLQ
                     LQAAELDPSRPVRGEEASAFLREGANRVQLVARLHRLLARTSDDAAPDLAEFLGDIVE
                     ATVALLPAGEARQIRYQGAGPCIMAAEKALPIGLMVGELLTNSLKYAHPTGVPGVITL
                     CCCRTVEGDLLVSVGDDGVGLPEGLDPTEASGLGLRLVNALARQVGGCLSFEQDGVGL
                     TVSLRVPPHATPGAGPD"
     gene            5787..6017
                     /locus_tag="PLO65_03080"
     CDS             5787..6017
                     /locus_tag="PLO65_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27264.1"
                     /translation="MSELNRGERLQIMLTDEELSALDDWRFARRMPSRAAAIRELLKR
                     GLSAEGFAETDGKQKSKDFGVTDGPRTAKADD"
     gene            complement(6067..7377)
                     /locus_tag="PLO65_03085"
     CDS             complement(6067..7377)
                     /locus_tag="PLO65_03085"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRD27265.1"
                     /translation="MAGETNKAPLRERQAKGRKRAARRDLLLEEAARRINAQGAGAIV
                     LNDIAEQVGLSRNTLYYYVADRADLVFRCYLRACEATADDLALAHELGADPADRIRRF
                     VAAELDFERPAQAVLTDVDYLPEPQRGLIREQQGRNLSALRGLIADGVADGAFRACDT
                     EIAAQTLLGLLSWARLSAGWLDYRDDRAARRRASDAIADLFLNGFAADPGAETRCAIS
                     ADTLVARPFNAFDRRQTNEVKIGQLIAAASRLFNRRGIDGVSLDEISASVGATKGAVY
                     HYFDDKADLVVRCYRRAFELFDLFMDTAVATGRTGFERALISLHLNVQAQASGLSPLM
                     LQPGRLSLPADELARFSAASRRLRMTSTRNMRQGLADGTCRPVDVSFASDVAAGIFLW
                     LPKWLPEGYPLSATRIADEIADLVALGVMRRTGPANAPPPTAPA"
     gene            7561..9816
                     /locus_tag="PLO65_03090"
     CDS             7561..9816
                     /locus_tag="PLO65_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27266.1"
                     /translation="MTFQKQGARGRRAALAGGAGLLALLVAGAAQAADAPAATDVDVV
                     TVTAQKRAQDTLDVGVNVAVISAEALEANRVTQVSDLSAFTPNVDIKENMPGILPVVT
                     IRGVGLNDFSATNNPSAGVYVDEVYLSSLALMNFDLFDVERMEALKGPQGTLYGRNST
                     AGAINIVTARPSFGGVEGRITGGLGNYETAEVDGMVNLPLSDSFALRISGKAVRQDEG
                     MFFNRVTGRDFGRRDVLMGRATARWAPSEDLDIQLKLEAQRVRSEVGAPEIFGLLTPP
                     GAPPGLVCPGSSGCMDFLGYTDTDNDPFRGDYSIDPTYDVDQRAATLRIERDFGWATL
                     TSITGYLEFEREWGADTDAGPYRQTDFIEHDNITQLSQELRLAGSTDRTDWLVGAFAS
                     RDRVRMTYDGDLQDLFNTTTLTFSNQTSKSAAVFGNVEYDLGHDVSLVAGLRYTWEEK
                     ANEGGDYDLVPNCPASFLTLTPCGAPPVLMASVDETISDTNWSWKLGFNWKPDPLTLV
                     YASVSRGVKSGGFFSGVATNSGQLQPYDPESLIAWEAGVKQRLPGAGLSWSGSVFYYD
                     YSDVQTFIRDTSGGLPIQRLGNVDEASIYGVDLDAMWSPAALPGLDLTAGLGLLKTEL
                     GAFASSGGAVPAGNELPDAPEVSFNLAAAWTVPVGDAMDLRFRIDGRYAGDMFKDALN
                     DPLIATDAYWVWNGRASLLSGSDWDISVWGKNLADEEYVTQGVNNLALGIGFRVYGAP
                     RTFGVSATRTF"
     gene            9816..10964
                     /locus_tag="PLO65_03095"
     CDS             9816..10964
                     /locus_tag="PLO65_03095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27267.1"
                     /translation="MGAVLADPDVQGTASPGFAALADAFARNFTERGDVGASLAVVHD
                     GEMVVDLWAGSLDAAGARPWSRDSVVNVWSTTKAVVALCFAMLVERGQASYDAPVARW
                     WPEFAAGGKGGVTLAQLLSHQAGLCSFTEPAVIEDYYDQRTAEARLAAQTPFWPPGSR
                     SGYHAISIGTLANGLFRRIEGRGLGQFIAEELSGPFGLDLTLGLPESEARRRAEMIAP
                     PELVTSNMATDLNPSQRAAYLNPVVEPLLPNTAAWRACEIPSANGFSNARSLARLFGA
                     LAGDGALDGRRLIGRETLAQATTERIAGVDEVLGVPARWGAGFLLNTDGLYGPQALAF
                     GHSGWGGSFVLVDPVRRMAVAYAMNRMGTDLVGDPRDVALIAAAYEGA"
BASE COUNT         1868 a         3693 c         3726 g         1688 t
ORIGIN      
        1 cccgaagccg cgcgctgccc cgaggccgcc gccccccgct tcagccgctg caaggccgcg
       61 ggcggccagc gcttcccgcg gcctcgccag gccgtagccg cccagtgctc cggccaggca
      121 gcaggcgaac accgcaagca cataggggat caacgggtca ctccgcaggg tggcggacgg
      181 cttcgacatt gaggccgccg gggtcgagca ccaaggctcc atggcaattg ggatgatagt
      241 ggggccgaag gccggtcgcg gcgcggaaac gccaggaccg cgcgatatcg ctgacgagcg
      301 cgccgacgtg atcgagcatg gcttcctccc tcactccgct cgccgcacaa gagcggcgat
      361 cccttgactc cgcaaccccg cgatcctaac tgcgcaccgt cgttggcact cgggagccag
      421 gactgctaac agcagccatc cgggggccgg cgaagaccat tcaaaccagc catacgcacg
      481 gagacgaccc gatgaagttt cgtcctcttg gcgaccgcgt ccttgtgaag cgtgtcgagg
      541 aagaagagaa gaccaagggc gggatcatca tccccgacac cgccaaggaa aagccgcagg
      601 aaggcgaagt gatcgccgtc gggcccggcg cccgggacga ttccggcaag gtccacgccc
      661 tggacgtgaa ggccggcgac cgcatcctgt tcggcaagtg gtccggcacc gaagtgaagg
      721 tgaacggcga ggatctgctg atcatgaagg aaagcgacgt cctgggcgtg gtcgagaagt
      781 aacccttctc cctcgcgctt cgcttcccaa tttccccatt agaaagagag ctccgaaatg
      841 gccgccaaag acgtctattt ctcttccgac gcgcgcgatc gcatgctgcg cggcgtcaac
      901 atcctcgcca acgcggtgaa ggtgaccctc ggccccaagg gccgcaatgt cgtgatcgaa
      961 aagtccttcg gcgccccgcg ctcgaccaag gacggcgtgt cggtcgccaa ggaaatcgaa
     1021 ctgagcgatc gtttcgagaa cctcggcgcc cagttgatcc gtgaagtcgc ttccaagacc
     1081 aacgacaagg ccggcgacgg caccaccacc gccaccgtcc tggcccaggc gatcgtcgtc
     1141 gaaggcctca agtcggtcgc ggccggcatg aacccgatgg acctgaagcg cggcgtcgac
     1201 aaggccgtga ccaaggtcgt ggaagagatc aagaactcct ccaagaaggt caccaccaac
     1261 caggaaatcg cccaggtcgg cgccatctcc gccaacggcg acgtggaaat cggcgacatg
     1321 atcgccaagg ccatggacaa ggtcggcaac gaaggcgtca tcacggttga agaagccaag
     1381 accgccgaga ccgagctcga cgtcgtcgag ggcatgcagt tcgaccgcgg ctacctgtcg
     1441 ccctacttca tcaccaacgc cgacaagatg gaaacgcagc tcgaagagcc gctgatcctg
     1501 ctgttcgaaa agaagctgtc gggcctgcag cccctgctgc cggtgctgga agccgtcgtg
     1561 cagtcgggcc gtccgctgct gatcatcgcc gaggacatcg aaggcgaggc cctggccacc
     1621 ctggtggtca acaagctgcg cggcggcctg cgggtcgcgg cggtcaaggc gccgggcttc
     1681 ggtgatcgcc gcaaggcgat gctggaagac ctcgccatcc tgaccggcgg ccaggtgatc
     1741 agcgaggacc tcggcatcaa gctcgagaac gtcaccctcg acatgctggg caaggccaag
     1801 aaggtcacca tcaccaagga cgacaccacg atcgtcgacg gttcgggcga caaggacggc
     1861 atcgaggccc gcatcgccca gatcaagcgc cagatcgagg acaccaccag cgactacgac
     1921 aaggaaaagc tgcaggaacg cctggccaag ctggccggcg gcgttgcggt ggtccgcgtc
     1981 ggcggcgcca cggaagtgga agtgaaggaa aagaaggacc gcgtcgacga cgccctcaac
     2041 gcgacccgcg cggccgtgga agaaggcatc gttcccggcg gcggcgtctg cctgctgaag
     2101 gcctccaagg ccctcgaaag cctgaccggc gacaacgccg accagacggc cggcatcgcc
     2161 atcgtccgcc gcgccctgca ggctccgatc cgccagatcg ccgagaacgc cggcgtcgaa
     2221 ggctcgatcg tggtcggcaa ggtgctggac ggcaaggacg cttccttcgg cttcaacgcc
     2281 cagacggaag agtacggcga cctggtcaag atgggcgtca tcgacccggc caaggtcgtg
     2341 cgcaccgccc tgcaggacgc ggcctcggtc gccggcctgc tgatcaccac cgaagccgcc
     2401 atcgtcgaag ccccgaagaa ggcctcggcc ggcgccgcgg gcggcatgcc cggcggcggc
     2461 atgggcggca tgggcgacat ggacttctag tccacgaaac caggctgaac gaaatgaagg
     2521 gcggttccgg aaacggagcc gccctttttc gtttcgttcc tctcccggag ggggctgcta
     2581 ctcgccttct tcccgcgggc cgatcagcat gtggccaaac cagctggtca cgccgacgac
     2641 cacggccgcg aacagcgccg gccagaagcc ggcgacggtg aagccgtcca tcagccaggc
     2701 ggtcagggcg atcatgccgg cgttcaccac cagcaggaac aggccgagcg tcacgagcgt
     2761 caacggcagg gtgaagaaga tcacgatcgg ccgcacgatg gcgttgacca cgcccagcac
     2821 cagggcggcg atcaccaggc tctgggtgtc ggcgaaatga atgccgggca cgaacttctg
     2881 cgccagccac agcccggcca tgccgaacag ggtgcgcagc gccagtctga cgagaagcat
     2941 gggcgcgtct ccactggccc tcccgatgaa ggggaggacg atgtgtctag tcagccggca
     3001 gcaggttctc gttgcgccgg gccatcagct tgtcgaactc gccccagacg ccagcctcgc
     3061 cgtgccactg gccgcgggtg gcggccttgg aatactcggt cgagcgggcc tcgaagaagt
     3121 tggcgtgctc gacgccgctg agcagcgact gcagccaggg cagggggttc tccgtcacgc
     3181 cgtacagctc gggcagcttc agctggcgca ggcgccagtc ggcgatgaag cggatgtact
     3241 gcttgatgtc ctcgggattc atgccctgga cgtcgccggc ctcgaaggcc aggtcgatga
     3301 accggtcctc gaggtgcacc acgtggcggc agcactcgac gatatcgtcg gccacgctct
     3361 tggtcactgc gccggtttcc ttgttgaagg cgtggtagag cttgatgatc ccctcgcagt
     3421 gcaggctctc gtcgcgcacc gaccagctga cgatctggcc catgcccttc atcttgttga
     3481 agcgcgggaa gttcatcagc atggcgaagg acgcgaacag ctgcagcccc tcggtgaagc
     3541 cgccgaacat ggccagggtg cgggcgatgt cggcgttatt ctcgacgccg aactgctgca
     3601 tgtagtcgtg cttggccttc atggcctcgt attccatgaa ggcgccgaac tcgctctccg
     3661 gcatgccgat ggtctcgagc agcagggcgt aggcggcgat gtggatggtc tccatgttgg
     3721 cgaagctgga gagcatcatc ttcacttcgg tcggccggaa gacgcggccg tagcggtcca
     3781 tgtagttgtc ctggacctcg atgtcgccct gggtgaagaa gcggaagatc tgcgtcagca
     3841 ggttgcgctc gcggtcgttc agattggccg cccagtcctt gcagtcctcg cccagcggca
     3901 cctcctcggg catccagtgg acctgttgct gccgcttcca catgtcgtag gcccacggat
     3961 agcggaacgg cttgtaggcc gccgacgggg tgagcaggcc gggcagggtg agcttggacg
     4021 aggcggtcac ggtgacgaac tccggacggg gacggacggg aatcgaggca ggcgcgttag
     4081 gatggacaac cgtatcaaac tgccgcgaca catctagtgg tcacgctgac gcaacccaca
     4141 agatgttgtg agattgtgga taaaatcatg aaccaatcct tgatcgtcta tggcgccctg
     4201 gggtcgggca gcgtaccggt cgaagcggcg ctgacgctcc ttgacctacc cttcgaggtg
     4261 gtcgaggcgg ccacctggga acaggatccc caggtgctgg cgcgtgtcga ggcggtcaat
     4321 ccgctgttgc aggtgccggc cctggtgctg ccgtccggcg aggtcctgac cgagagcgcc
     4381 gcgatcctgc tttggctggc cgacgcccat cccgaggcgg gactggggcc cggcgttgac
     4441 gatccgcgcc gggcccagtt tctgcgctgg atgaccttca tcccggcgtc gatctattcg
     4501 atgttctggg tccgcgatgt ccccgcgcgc ctggcgggcc ccgatccggt ccagggggag
     4561 cgggtcaagc aggcgacggc cgagcgcatc gccgactgct ggcgactgat ggaccggcag
     4621 atcacgccgg ccggcgccta tctgctcggc gacacgcttt cggtgctcga cctctatgtc
     4681 gccgtggtca gccgctgggg gcccggccgc aaccgcttct accgggaggc gccggggatg
     4741 acgccggtgg tccgccgcgt cgacgccgac ccgcggctgg cggatttctg ggcgcgccgc
     4801 tttccgttcg cggccggctg ggaacgctag tcgggcccgg cccccggcgt ggcgtgcggg
     4861 ggaacccgca ggctcacagt cagcccgacc ccgtcctgct caaagctcag gcatccgcca
     4921 acctggcggg cgagggcgtt gaccagcctc aggcccaggc ccgacgcctc ggtcgggtcc
     4981 agaccctcgg gcaggcccac gccgtcgtcc ccgaccgaca cgagcaggtc gccttcgacg
     5041 gttcgacagc aacacagggt gatcaccccg ggcacgcccg tggggtgggc gtatttcaga
     5101 ctattggtca gcaattcccc gaccatcagg ccgatgggca gggccttttc ggccgccatg
     5161 atgcaggggc cggccccctg gtaacgaatc tggcgagcct cgcccgcggg cagcagggcg
     5221 accgtggcct cgacgatgtc gcccagaaac tcggccagat cgggcgcggc gtcgtccgag
     5281 gtccgcgcca gcaaccgatg cagccgggcc accagctgca cgcggttggc gccctctcga
     5341 aggaaggcgg aagcctcctc gcccctcacc ggccgggagg ggtcgagctc ggcggcctgc
     5401 aactgcagca ggcccgcgac gaggctgagg ttgttggcga tgcggtggtt ggcctcctgg
     5461 agccacatct ccgctccgcc gtcgcgactc ggcttgtggg cgccgaaggt ggcgaggagc
     5521 aggttgtggg ccatgaggtt ctctcaaata ttatatcgac gcccgcgatc aatatagccc
     5581 gaatagagaa aaacacaaat gaaaagggga tccaagatat tgataaggct aaagtatatg
     5641 cagatagcgg cctatatgtc ttctgctgtt ctcaccgtcg gtttgtcgaa tgagtaaaat
     5701 gtgccttttg caaccttctg gcccggtagc agtttcagga gcgcaggcag ggctccggga
     5761 atggccgggc gatggaggcg agcagagtgt ctgaactgaa caggggcgag cggctgcaga
     5821 tcatgctcac ggacgaggag ctgtctgccc tggacgactg gcgcttcgcg cgccgcatgc
     5881 ccagccgtgc ggccgcgatc cgtgagttgc tcaaacgcgg tctctccgcc gagggcttcg
     5941 ccgaaaccga cggaaagcag aagtccaagg acttcggggt caccgatggt cccaggaccg
     6001 ccaaggcgga cgactgacgc tcccgccggc cctgccgtgt cgagagccgc cgagaccggc
     6061 agcctgtcag gcgggtgcgg tgggcggcgg ggcgttggcc gggcctgtcc tgcgcatgac
     6121 gcccagggcc accagatcgg cgatttcgtc ggcgatccgg gtggccgaga gcggatagcc
     6181 ctcgggcagc catttcggca gccacaggaa gatgccggcg gccacatcgc tggcgaacga
     6241 gacgtcgacg gggcggcagg tgccgtccgc cagcccctgt cgcatattgc gggtcgaggt
     6301 catccgcagg cgtcgtgacg cggcgctgaa ccgcgccagt tcatccgccg gcagcgacag
     6361 ccgtccgggc tgcagcatca gcggcgacag gccgctggcc tgggcctgga cgttcaggtg
     6421 caggctgatc agggcgcgct cgaatccggt tcggcccgtg gccacggcgg tgtccatgaa
     6481 caggtcgaac agctcgaagg cccggcgata gcaccgcacc accaggtcgg ccttgtcgtc
     6541 gaagtaatgg tagacggcgc ccttggtggc cccgacgctg gcgctgatct cgtcgagcga
     6601 aacgccgtcg atcccgcgcc gattgaacag ccgcgaggcc gcggcgataa gctggccgat
     6661 cttgacttcg ttggtctgcc gccggtcgaa ggcgttgaac ggtctcgcga ccagcgtgtc
     6721 ggcgctgatc gcgcaacggg tctcggcccc cggatcggcc gcgaacccgt tgaggaagag
     6781 atcggcgata gcgtcgctgg cccgacggcg cgcggcgcgg tcgtcacggt aatcgagcca
     6841 cccggccgaa aggcgcgccc aggacagcag gcccagcagc gtctgggccg cgatctcggt
     6901 gtcgcaggcg cgaaacgcgc cgtcggcgac gccatcggcg atcagccccc gcagggcgga
     6961 aagattacgg ccctgctgtt cccggatcag cccccgttgc ggttcgggca ggtagtcgac
     7021 atcggtcagc acggcctggg cggggcgctc gaaatccagc tccgcggcga cgaaacggcg
     7081 aatgcggtcg gcgggatcgg cgccgagttc gtgggccagc gccagatcgt cggccgtcgc
     7141 ctcgcaagcc cgcaggtagc agcggaacac caggtcggcc cggtcggcga cgtaatagta
     7201 gagcgtattg cgcgacagcc cgacctgttc ggcgatgtcg ttcagcacga tggcgccggc
     7261 gccctgggcg ttgattcgcc gcgccgcctc ctcgagcagc aggtcgcgcc gggcggcgcg
     7321 cttgcggccc ttcgcctgcc tttccctcag cggcgccttg ttggtctcgc cggccatgga
     7381 gccctttcgc cgtccggtcc gcgactgatg ttgttcgacg atctatagcc tgaaacctac
     7441 ctgactgtcg aaaaacttga cgccccgatc tcgtcgtgtc gttatcaata caaactcaaa
     7501 tgtcgaaaaa ccggcaacct gcgggtcagc cgggacgacc atcagggagg agccggggac
     7561 atgacttttc agaagcaggg ggcgcgtggc cgcagggccg cgctggcggg cggcgccgga
     7621 ttgctggcgt tgctggtcgc gggcgcggcg caggcggccg acgcgccggc ggcgaccgac
     7681 gttgatgtcg tcaccgtcac ggcccagaag cgggcgcagg atacgctcga cgtcggcgtc
     7741 aatgtcgccg tgatctcggc cgaggcgctc gaggccaacc gcgtgacgca ggtcagcgac
     7801 ctgtcggcct tcacccccaa cgtcgacatc aaggagaaca tgcccggcat cctgccggtg
     7861 gtgaccatcc gcggcgtcgg cctgaacgac ttcagcgcga ccaacaatcc cagcgccggc
     7921 gtctatgtcg acgaggttta tctgagctcg ctggcgctga tgaacttcga cctgttcgat
     7981 gtcgagcgga tggaggcgct gaaagggccg cagggtacgc tctacggccg caactcgacc
     8041 gccggggcga tcaacatcgt caccgcccgg ccgagcttcg gcggggtcga gggacggatc
     8101 accggcggtc tgggcaacta cgagacggcg gaggtcgatg gcatggtcaa cctgccgctg
     8161 tccgacagct tcgcgctgcg catcagcggc aaggccgtgc gccaggacga gggcatgttc
     8221 ttcaaccgcg tgacaggccg cgatttcggc cgtcgcgacg tgctgatggg ccgggccacc
     8281 gcgcgctggg cgcccagcga ggatctcgac atccagctca agctcgaggc gcagcgcgtg
     8341 cggtccgagg tcggggcgcc ggagatcttc ggcctgctga ccccgccggg ggcgccgccg
     8401 ggactggtct gcccgggctc gtccggctgc atggacttcc tgggctatac cgataccgac
     8461 aacgacccgt tccggggcga ctactcgatc gacccgacct atgacgtcga ccagcgggcc
     8521 gccacgctgc gcatcgagcg cgacttcggc tgggccaccc tgacctcgat cacgggctat
     8581 ctcgaattcg agcgtgaatg gggcgccgac accgacgccg gcccgtatcg ccagaccgac
     8641 ttcatcgagc acgacaacat cacccagctg tcgcaggagt tgcgcctggc tggatcgacc
     8701 gaccgcaccg actggctggt cggcgccttc gcctcgcgcg accgggtcag gatgacctat
     8761 gacggcgacc tgcaggacct gttcaacacc acgaccctga ccttctccaa ccagacctcg
     8821 aagtcggcgg cggtgttcgg caatgtcgaa tacgacctcg gccacgacgt cagcctggtc
     8881 gccggcctgc gctacacctg ggaagagaag gccaacgagg gcggcgacta cgatctggtc
     8941 cccaactgcc cggccagctt cctgaccctc acgccctgcg gcgcgccgcc ggtgctgatg
     9001 gccagcgtcg acgagacgat cagcgacacc aactggtcgt ggaagctcgg cttcaactgg
     9061 aagcccgacc ccctgaccct ggtctacgcc agtgtcagcc gcggggtgaa gagcggcggc
     9121 ttcttctccg gcgtggcgac caattccggc cagctgcagc cctatgatcc ggaaagcctg
     9181 atcgcctggg aggcgggcgt gaagcagcgt ctgcccggcg cgggcctgtc ctggtccggc
     9241 agcgtcttct attacgacta ctccgacgtc cagaccttca tccgcgacac ctccggcggc
     9301 ctgccgatcc agcggctggg caatgtcgac gaggccagca tctatggggt cgacctcgat
     9361 gcgatgtggt cccccgcggc gctgccgggg ctcgatctca ccgccggact ggggctgctg
     9421 aagaccgagc tgggcgcctt cgcctcgtcc ggcggcgctg tcccggccgg caacgagctg
     9481 cccgacgcgc cggaggtcag cttcaatctc gccgccgcct ggaccgtgcc cgtcggcgac
     9541 gccatggacc tgcggttccg catcgacggc cgctacgccg gcgacatgtt caaggacgcg
     9601 ctgaacgatc cgctgatcgc caccgacgcc tactgggtgt ggaacggccg cgcgagcctg
     9661 ctgtcgggat cggactggga catctcggtc tggggcaaga acctcgccga cgaggaatac
     9721 gtgacccagg gcgtcaacaa cctggcgctg ggcatcggct tccgcgtcta cggcgcgccg
     9781 cgcaccttcg gcgtcagcgc cacgaggacc ttctgatggg cgccgtgctt gccgaccctg
     9841 acgtccaggg gaccgcttcg cccgggttcg cggcgctggc cgacgccttc gcgcgaaact
     9901 tcaccgagcg cggcgacgtc ggcgcctcgc tggccgtggt ccacgacggc gagatggtcg
     9961 tcgacctgtg ggcgggcagt ctcgacgcgg ccggggcccg gccgtggtcg cgcgactcgg
    10021 tggtcaacgt ctggtcgacg accaaggccg tcgtggccct gtgcttcgcc atgctcgtcg
    10081 agcgcggcca ggcgtcctac gacgcgccgg tcgctcgctg gtggccggag ttcgcggccg
    10141 gcggcaaggg cggcgtcacg ctggcccagc tgctgtcgca tcaggccggc ctgtgcagct
    10201 tcaccgagcc cgcggtgatc gaggactact acgaccagcg cacggccgag gcgcggctcg
    10261 cggcccagac gccgttctgg ccgccgggct cgcgctcggg ctaccacgcc atcagcatcg
    10321 ggaccctggc caacggcctg ttccggcgga tcgaggggcg cggtctgggc cagttcatcg
    10381 ccgaggagct gtccgggccg ttcggcctgg acctgacgct cggcctgccc gagtccgagg
    10441 cccgccgacg ggccgaaatg atcgccccgc cggagctggt cacctccaac atggccaccg
    10501 acctgaaccc ctcgcagcgc gccgcctacc tcaacccggt agtcgagccg ctcctgccca
    10561 acaccgccgc ctggcgggcc tgcgagattc cgtcggccaa cggcttctcg aacgcccggt
    10621 cgctggcccg gctgttcggc gcgctggccg gcgatggcgc cctggacggc cgtcgcctga
    10681 tcggtcgcga gaccctggcc caggcgacga ccgaacgcat cgcgggcgtc gacgaggtgc
    10741 ttggggtccc ggcccgctgg ggggcgggat tcctgctcaa taccgacggc ctatatggcc
    10801 cgcaggcact cgccttcggt cactccggct ggggcggctc gttcgtgctg gtcgacccgg
    10861 tccgccggat ggccgtcgcc tacgccatga accgcatggg cacggacctg gtgggggacc
    10921 ctcgcgacgt ggcgctgatc gcggccgcct acgagggcgc ctgagacgcg ccccc
//
LOCUS       DAORNX010000041        10918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4504_length_10918_cov_8.864034, whole genome shotgun sequence.
ACCESSION   DAORNX010000041 DAORNX010000000
VERSION     DAORNX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_10918_cov_8.864034"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLO65_03100"
     CDS             complement(<1..389)
                     /locus_tag="PLO65_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRD27268.1"
                     /translation="MAEFAPDPAFGPGSMALGELPLCHVRLQTDARWPWLILIPRGIG
                     LREIEDLPAVDRARLIEESVLAGQAVRAMGAALGRPVEKLNLGALGNVTAQLHVHLVG
                     RHAGDAAWPGPVWGVGTAEAYGPEALDL"
     gene            complement(382..1431)
                     /locus_tag="PLO65_03105"
     CDS             complement(382..1431)
                     /locus_tag="PLO65_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD27269.1"
                     /translation="MSKTDPGNYFEDFRLGQVLDHATPRTVTAGDVAVYNALHGPRFA
                     LTSSDEFARACGLRASPVDALVAFHMVFGKTVPDISLNAVANLGYAEGLFLKALYPGD
                     TVTATSEVIGLKENSNRKTGVVYVRTTGENQHGETVLSYVRWVMVRKRSEAAEVPEQA
                     TPKLAEAVAAKDLVLPHGLDFSRYDFTLAGAPHAFEDYAIGEKIDHVDGMAIEEAEHA
                     MATRLYQNTAKVHFNQYERAKDPTGRRLVYGGVVISTAKALSFNGLQNAGLMLAINGG
                     RHVNPYFAGATVFAWSEVLDKADLGEGVGALRLRLVATKDRPCADFPDKDETGAYPPE
                     VILDFDYWAAVPKRG"
     gene            1665..2627
                     /gene="mdh"
                     /locus_tag="PLO65_03110"
     CDS             1665..2627
                     /gene="mdh"
                     /locus_tag="PLO65_03110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRD27270.1"
                     /translation="MARAKIALIGAGMIGGTLAHIAAREELGDIILFDIAEGTPQGKA
                     LDIAEASAVFGKDIALKGANAYEDIAGADVCIVTAGVPRKPGMSRDDLLGINLKVMKA
                     VGEGIKAHAPNAFVICITNPLDAMVWALQQFSGLPKAKVIGMAGVLDSARFAYFLAEK
                     TGVSVEDIHAWTLGGHGDDMVPMVRHSTVGGLPLPELVKQGWMTQDELDGIVKRTRGG
                     GGEIVALLKTGSAFYAPAESAIAMATSYLKDKKRVLPCATFLTGQYGLKDLYVGVPVV
                     IGAGGAERVIEFETNDEEKAMFAASVASVQGLMEACKAIDPSLV"
     gene            2868..3416
                     /locus_tag="PLO65_03115"
     CDS             2868..3416
                     /locus_tag="PLO65_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27271.1"
                     /translation="MIAPGPTLETARLILRPTGQEDLDGWAEMMADEAASRFIGGLQG
                     RAQSWRGLASMAGSWAIKGFAMFSVIEKSSGAWVGRIGPWQPEEWPGTEVGWGLHPRA
                     WGKGYAVEAATAAIDWAFDTLGWAEVIHCIDPGNVNSQKVAQRLGSSNRGEGRIPPPF
                     HELPIEIWGQSREAWLARRALR"
     assembly_gap    3560..3784
                     /estimated_length=225
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3847..4668)
                     /locus_tag="PLO65_03120"
     CDS             complement(3847..4668)
                     /locus_tag="PLO65_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRD27272.1"
                     /translation="MAQSDMRGEQAAVRERTGAAVRASWIATLLTNRHARGLALAALA
                     GVFLALVGAFGTSDAPVGLRLTYWITVMLIGALVGLLVSTLVARGGWFDDRPVLQGSL
                     IAIALTVPLTLVIWLMTAVIFGGGAPHLAALRYYAGPVFIVTTAMTALNYLAQKTPAE
                     THAAPAGAAPPRFLERLPPKLRGAEIFAVEAEDHYLRLHTSKGQDLILMRLSDAVAEL
                     EGLEGAQVHRSWWVARAAVADARRGDGRATLTLTDGAEVPVSRAYAKALRASGWI"
     gene            4787..5320
                     /locus_tag="PLO65_03125"
     CDS             4787..5320
                     /locus_tag="PLO65_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRD27273.1"
                     /translation="MTQSPFARTLPASRLAGTVLALAAGVATVVLFGPQMLESVLHAT
                     PHGPDVSLFMAQPAVIKIHMLAALVGVGLGATILSLRKGNTLHRTLGWTWTLFMATAA
                     ASSLFIVGLNGNHWSPIHLLSGWTLIILPFAIRAARKHDVMSHRRAMTSLFWGASIVA
                     GAFTFLPGRLMWRLFFG"
     gene            5401..6096
                     /locus_tag="PLO65_03130"
     CDS             5401..6096
                     /locus_tag="PLO65_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD27274.1"
                     /translation="MTSAKPRVRNTGRGRPSKGSGVDLKEVILDAAEGLFSRHGFYGV
                     TTRQVATEAGVDTALIHYYFGAKRELFDAVLLRRAEIINRDRADAMSAYEAANAGSMT
                     AEGVIAAFVDPLIERALTGGSGWKNWLNLVAQINITPAWGGEVMHRFFDPVVRQLVET
                     LARALPGADPRDLYWCYQFLTGSMMLTVAETGRIDQLSDGLCQSSDLEAARQRLYAYC
                     AAGFEAAVAKGRR"
     gene            6302..8485
                     /locus_tag="PLO65_03135"
     CDS             6302..8485
                     /locus_tag="PLO65_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27275.1"
                     /translation="MKSMWKSMLLAGVAWSAVSGAAVAQEATDTQVEEVVVTARRREE
                     ALKDVPVAVSAFSGEKLETSGAADITDLQRTVPSLTLQVARGSNSTLISFIRGVGQQD
                     PLWGFDPGVGLYVDDVYIARPQGAVLDIFDVSRIEVLRGPQGTLYGRNTIGGAIKYVT
                     SHIGSEPELKVRGSLGSYGQRDLIVSGEMPVSDTFSISGAVAKYDRDGYGTNKFTGAE
                     HYNKDVLAARGSIEFQPTESLFFRFSADMVRDDSNPRHGHRELAPSPSNVYDTYAGAG
                     DSNSVESRGLSFLGEWTINDNVTLKSISAYRAGETDGVIDFDNTPSPTLDIPARYRDH
                     QFTQEFQLLLEGEKWNGVAGLFYLNATASGAFDTVLGLANLTIATAGEVNTRSYAAFA
                     DFSYDFTDRFSVSVGGRLTRDVKDGYVYRQNFTGIRSPLFGNTAAIPGLLRTDPALYA
                     GKQLDWKEFTPRVSASYKLTPDLTAYASWGKGFKSGGFDMRGDYVSYPGTVDGYDPEF
                     VSTTELGLKGALFGNRLNFATAIFDSSYEDQQITTQYPSGLSVASVVDNVGSSSIQGW
                     EFEGSARLFDGFTANLMLSYIDASFDKYIAFIPGTGYVDVSNARDFQNTPDWSGSGGF
                     TYVHDLGDHGTLTFTPTASYRGDSQMFEVASPVLDQPAYWLYDASLVWASPDGQYKVG
                     LHGKNLGDEEYRVGGYNFPGALFGDSIIGYYGPPRTVTLTFDARF"
     gene            8556..9872
                     /locus_tag="PLO65_03140"
     CDS             8556..9872
                     /locus_tag="PLO65_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27276.1"
                     /translation="MNTGSKDATPAGGYRYYVLAMLIVVYTFNFLDRQILGILATPIK
                     AEFGLSDGQFGLMSGLAFALLYTTLGVPIALLADRFSRSWIMTAAFALWSGFTALCGV
                     VTSFPMLFLMRMGVGIGEAGGTAPAYSLIADYFPPSQRGRALAAFAFGIPAGMGAGTL
                     IGGLVAAQYGWRMSFIVVGLAGLALAIPFKLLVRDPPRGGQDSPPAPVAAAPFGKVLG
                     AVLPKPSFWLLSFGAASSSVCGYGVAAWLPSFFQRSFGLTLVETSWYAAGISILGGLL
                     GIWLGGAVADRLGATSKRAYPLTPAVAFLVAVPLFLAAMNSGPLLAGLGGGGSRLASL
                     ALGFGLFLIPTGLNLAWIGPITATVQHLVPPHMRATASALFLLINNLLGIAGGIYYFG
                     FMSDLLAPHFGTESLRWSIYTGMGFYLLSSALFVLASRVLEKDWQD"
     gene            10042..>10918
                     /gene="sucC"
                     /locus_tag="PLO65_03145"
     CDS             10042..>10918
                     /gene="sucC"
                     /locus_tag="PLO65_03145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRD27277.1"
                     /translation="MNIHEHQAKAVLAEFGVPVPRGFAAFTPDEAATAAETLGTPVFV
                     VKSQIHAGGRGKGKFEGLGPDAKGGVRVVKSVDEVRSNAAEMLGRVLVTHQTGPKGKQ
                     VNRLYIEEGAAIAKEFYLSLLVDRASSHVSVVASTEGGMDIEDVAHNTPDKIHTFTID
                     PATGVWPTHQRALAKALGLTGGLAKEAASLLTQLYTAFLAKDMAMLEINPLIVTADDH
                     LRVLDAKLSFDGNSLFRHPDIKALRDETEEDPKEIEASKFDLAYIALDGEIGCMVNGA
                     GLAMATMDIIKLYGAE"
BASE COUNT         1626 a         3854 c         3539 g         1674 t
ORIGIN      
        1 aggtccagag cctcgggccc gtaggcctcc gccgtcccga cgccccacac cggacccggc
       61 caggccgcgt cgccggcatg ccgtccgacc agatgcacat gcaactgggc ggtcacgttg
      121 ccgagcgcgc caaggttcag cttttccacc ggccggccca gcgccgcgcc catggcccgc
      181 acggcctggc cggccagcac cgattcctcg atcagccgcg cgcgatcgac ggccggcagg
      241 tcctcgatct cgcgcagccc gatcccgcgc gggatcagga tcagccaggg ccagcgggcg
      301 tcggtctgca gccgcacatg gcagagcggc agctcgccca gcgccatcga gccgggcccg
      361 aacgccggat cgggcgcgaa ctcagccacg tttgggaacc gccgcccagt agtcgaagtc
      421 gaggatcacc tcgggcggat aggcgccggt ctcgtccttg tccgggaagt ccgcgcaggg
      481 ccggtccttt gtcgccacca gccgcaggcg cagggcgccg acgccctcgc cgaggtcggc
      541 cttgtcgagc acctcgctcc aggcgaacac cgtggcgccg gcgaaatacg ggttcacatg
      601 ccgcccgccg ttgatcgcca gcatcaggcc cgcgttctgc aggccgttga agctcagggc
      661 cttggcggtc gagatcacca ccccgccgta gaccagccgc cggccggtcg ggtccttcgc
      721 gcgctcatac tggttgaaat ggaccttggc ggtgttctga tagaggcgcg tcgccatggc
      781 gtgctcggcc tcttcgatcg ccatgccgtc gacgtggtcg atcttctccc cgatggcgta
      841 gtcctcgaag gcgtgcggcg cgccggccag ggtgaagtcg tagcggctga agtcgaggcc
      901 gtgcggcagg accaggtcct tcgccgccac cgcctcggcc agcttcggcg tcgcctgctc
      961 cgggacctcg gcggcttcgc tgcgcttgcg caccatcacc cagcgcacat agctcagcac
     1021 cgtctcgccg tgctggttct cgccggtggt ccgcacatag accacgccgg tcttgcggtt
     1081 ggagttctcc ttcagtccga tgacctcgct ggtcgcggtc accgtgtcgc ccggatacag
     1141 cgccttgagg aacagcccct cggcgtagcc caggttggcg acggcgttca ggctgatgtc
     1201 cggcacggtc ttgccgaaga ccatatggaa ggcgaccagg gcatcgaccg gtgaggcgcg
     1261 caggccgcag gcgcgggcga actcgtccga cgaggtcagg gcgaagcgcg ggccgtgaag
     1321 tgcgttgtat acggccacat cccccgccgt gaccgtgcgc ggcgtggcgt ggtcgagcac
     1381 ctgaccgagg cggaaatctt cgaagtagtt gccggggtcg gtcttggaca tggggggctc
     1441 cttcagtgcg tccttcgaca cgcaccctgc gggtgctgct caggatgacg taattcagcg
     1501 gctcacaaac gaacgtcatc ctgagcaggc cgcgccagcg gccgtgtcga aggacgcaat
     1561 gacggtaatg ccgcgctctt cgcatttgcg aaagaggctt gaggcggaac cgcgcgaggg
     1621 ctagaccccg cgcgaccctt ttcgacaatt cggagacgcc caagatggct cgcgcgaaga
     1681 tcgccctcat cggcgccggc atgatcggcg gcaccctggc ccacatcgcg gcgcgcgagg
     1741 agctgggcga catcatcctg ttcgacatcg ccgagggcac cccgcagggc aaggccctcg
     1801 acatcgccga ggccagcgcc gtgttcggca aggatatcgc gctgaagggc gccaacgcct
     1861 acgaggacat cgccggggcc gacgtctgca tcgtcaccgc cggcgtgccg cgcaagccgg
     1921 gcatgagccg cgacgacctg ctgggcatca acctgaaggt catgaaggcc gtcggcgagg
     1981 gcatcaaggc ccatgccccc aacgccttcg tcatctgcat caccaacccg ctcgacgcca
     2041 tggtctgggc cctgcagcag ttctcgggcc tgcccaaggc caaggtcatc ggcatggccg
     2101 gcgtgctcga ctcggcgcgc ttcgcctact tcctggccga gaagaccggc gtgtcggtcg
     2161 aggacatcca cgcctggacg ctcggcggcc acggcgacga catggtgccg atggtgcgcc
     2221 actcgacggt cggcggcctg cccctgccgg aactggtcaa gcagggctgg atgacccagg
     2281 acgagctcga cggcatcgtc aagcgcacgc gcggcggcgg cggcgagatc gtcgccctgc
     2341 tgaagaccgg cagcgcgttc tacgccccgg ccgagtcggc catcgccatg gcgaccagct
     2401 acctcaagga caagaagcgc gtgctgccct gcgccacctt cctgaccggc cagtacggcc
     2461 tcaaggacct ctatgtcggc gtgccggtgg tcatcggcgc cggcggcgcc gagcgggtca
     2521 tcgagttcga aaccaacgac gaggagaagg cgatgttcgc cgcctccgtc gcctcggtcc
     2581 agggcctgat ggaagcctgc aaggcgatcg atccgtcgct ggtgtaaacc cgcttgtgaa
     2641 tcctccccgg cttgccgggg agggggaccg ccgaaggcgg tggaggggct gcgcaggggc
     2701 cagcggcccc gtcccgattc aggcctttcc ggcgcggccc gcgcggcccc tccaccatgc
     2761 tccgcatggt ccccctcccc tctgcgaggg gaggagcgcc gcgtcggatt tccccggcct
     2821 cacgaacccg gcgcccaatc gcgcgcggtt ttgctagaga cgggtccatg atcgctcccg
     2881 gaccgactct tgaaaccgcc cgcctgattc tgcgcccgac cggccaggag gacctcgacg
     2941 gctgggcgga gatgatggcc gacgaggcgg cctcgcgctt catcggcggg ctgcaggggc
     3001 gggcgcagag ctggcggggg ctggccagca tggccgggtc ctgggcgatc aagggtttcg
     3061 ccatgttctc ggtgatcgag aagtcgagcg gcgcgtgggt cggccggatc ggcccgtggc
     3121 agcccgagga gtggccgggc acggaagtcg gctggggcct gcatccccgc gcctggggca
     3181 agggctacgc ggtcgaggcg gcgaccgcgg ccatcgactg ggccttcgac acgctgggct
     3241 gggccgaggt gatccactgc atcgatccgg gcaacgtcaa ttcgcagaag gtcgcgcagc
     3301 ggctgggctc gagcaaccgg ggcgaggggc gcatcccgcc gccgttccac gagctgccga
     3361 tcgagatctg gggccagagc cgcgaggcgt ggctggcccg gcgggcgctc cggtgacccg
     3421 ggccggtctg ctgctggcgg cgcttctggc gcttgccccc gccgcggccg gcgcacgaag
     3481 cgaagacggg gccctgaccg tgtccttcga cggcgtcagc cggcgcgaag gcgtggtgct
     3541 ggtcgccctg ttcgacagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnccgcct tcgaggtcag gccgggcggc aatctccaga cgatccggct gcggtgacgg
     3841 tgcgagtcag atccacccgc tcgcccgaag cgccttcgca taggcccggc tgaccgggac
     3901 ttctgcgccg tcggtcagcg tcagggtcgc ccggccgtcg ccgcgccggg cgtccgccac
     3961 ggccgccctc gccacccacc aggagcggtg cacctgggcg ccctcgagcc cctcgagctc
     4021 ggcgaccgcg tcggacaggc gcatcaggat caggtcctgg cccttgctgg tgtgcaggcg
     4081 caggtagtgg tcctcggcct cgaccgcgaa gatctccgcg ccgcgcagct tcggcggcag
     4141 gcgctcgagg aatcgcggcg gcgcggcgcc ggcgggcgcg gcgtgagtct cggccggcgt
     4201 cttctgggcc aggtagttga gcgccgtcat ggccgtggtg acaatgaaca ccgggccggc
     4261 gtagtagcgc agcgccgcca ggtggggcgc gcccccgccg aagatgaccg cggtcatcag
     4321 ccagatgacc agcgtcagcg gcacggtcag ggcgatggcg atcaatgagc cctgcagcac
     4381 cggacgatcg tcgaaccagc ccccgcgggc gaccagcgtc gagaccagca ggccgaccag
     4441 cgcgccgatc agcatcacgg tgatccagta ggtgaggcgc agtccgaccg gcgcgtcgct
     4501 ggtgccgaac gcgccgacca gggcgaggaa cacgcccgcc agggcggcca gggccaggcc
     4561 gcgggcgtgg cggttggtca ggagcgtggc gatccacgaa gcgcgaacgg cggcgcctgt
     4621 ccgttcgcga acggcggcct gctctccacg catgtccgac tgcgccatcc ggccgcctcg
     4681 cctaagccat cgtcatcgcc ggggccgccg ggaggggcgt ccgcaatgac ctctcccgga
     4741 tcaagaccga ccctgtctcc ctgtcaatcg ttccgccgga gccctcatga cccaatcgcc
     4801 cttcgccagg accctgccgg ccagccgact ggccggaacc gtgctcgcgc tcgccgccgg
     4861 cgtcgccacc gtggtcctct tcggcccgca gatgctcgag tcggtgctgc acgccacgcc
     4921 gcacggtcct gacgtttcgc tgttcatggc ccagccggcg gtgatcaaga tccacatgct
     4981 ggcggcgctg gtcggcgttg gcctcggcgc gacgatcctg agcctgcgca agggcaacac
     5041 cctgcaccgg accctgggct ggacctggac cctgttcatg gcgacggcgg ccgccagctc
     5101 gctgttcatc gtcgggctga acggcaatca ctggtcgccg attcatctgc tcagcggctg
     5161 gaccctgatc atcctgccgt tcgccatccg ggccgcccgc aagcacgacg tgatgagcca
     5221 caggcgcgcc atgaccagtc tgttctgggg cgcgtcgatc gtcgcgggag ccttcacctt
     5281 cctgccgggc cggctgatgt ggcggctgtt cttcggctga accggccttg ccagcgggcg
     5341 agcacgcggc gtatcgcagg acgctctcac ctcggcgagc ccgtccggat cctgcccccg
     5401 atgacctccg ccaagccgcg cgtccgcaac accggccggg gccgcccgtc caaaggcagc
     5461 ggcgtcgatc tcaaggaagt gatcctcgat gccgccgagg gactgttctc gcgacacggc
     5521 ttctatggtg tcacgacccg gcaggtggcc accgaggcgg gggtcgatac ggccctgatc
     5581 cactactatt tcggggccaa gcgcgagctg ttcgacgccg tgctgctgcg ccgggcggag
     5641 atcatcaacc gtgaccgcgc cgacgccatg tccgcctacg aagcggccaa tgcggggtcg
     5701 atgaccgccg agggcgtcat cgccgccttc gtcgatccgc tgatcgagcg ggcgctgacc
     5761 ggcgggtcgg gctggaagaa ctggctcaac ctggtggccc agatcaacat cacgccggcc
     5821 tggggcgggg aggtcatgca ccgcttcttc gacccggtgg tgcgacagct ggtcgagacc
     5881 ctggccaggg ccctgccggg cgccgacccg cgcgatctct actggtgcta ccagttcctc
     5941 accggctcga tgatgctgac ggtggccgag accggccgca tcgaccagct gtccgacggc
     6001 ctgtgccagt ccagcgatct cgaagcggcc cgccagcgtc tgtacgccta ctgcgccgcg
     6061 ggcttcgagg ctgccgtggc caagggccgc cgctgacggt cctcggcaag ggcgtgtggc
     6121 gccaattcat cacctgatga aaaacttcat ccccggctca cgctgggcct tccgcgcgcg
     6181 tcagtcctga cggacagtcc gcacaattcg cgttctggtg aatttaacaa tcaaccgcgt
     6241 ccgtcaacgg accggcctga ccacttcggt cgggggcgac gccaactggg agggcaatcc
     6301 aatgaagtcc atgtggaaga gcatgctgct ggccggagtg gcctggagcg ccgtcagcgg
     6361 ggccgccgtg gcccaggaag cgaccgacac ccaggtcgag gaggtggtgg tcaccgcccg
     6421 ccgccgcgag gaagccctca aggacgtgcc ggtcgccgtt tccgccttca gcggcgaaaa
     6481 gctcgagacc agcggcgccg ccgacatcac cgacctgcaa cgcaccgtcc ccagcctgac
     6541 ccttcaggtg gcccgcggct ccaactcgac cctgatctcg ttcattcgcg gcgtcggtca
     6601 gcaggatccg ctgtggggct tcgaccccgg cgtgggcctg tatgtcgacg atgtctacat
     6661 cgcccggccg cagggcgcgg tgctggacat cttcgacgtc agccggatcg aggtcctgcg
     6721 cggcccgcag ggcacgctct acggccgcaa caccatcggc ggcgcgatca agtacgtcac
     6781 cagccacatc ggcagcgagc ccgagctgaa ggttcgcggt tcgctcggct cctacggcca
     6841 gcgggacctg atcgtcagcg gcgaaatgcc ggtgtccgac accttctcga tcagcggcgc
     6901 ggtcgccaag tacgaccgtg acggctacgg cacgaacaag ttcaccggcg ccgagcacta
     6961 caacaaggac gtgctggccg cgcgcggcag catcgagttc cagccgaccg agtcgctgtt
     7021 cttccgcttc tcggctgaca tggtccgcga cgattccaac cctcgtcacg gccatcgcga
     7081 gctggccccc agcccgtcca atgtctacga cacctacgcc ggcgccggcg acagcaactc
     7141 ggtcgagagc cgcggcctgt cgttcctcgg cgagtggacc atcaacgaca acgtgaccct
     7201 caagtcgatc tcggcctacc gcgccggcga gaccgacggg gtgatcgact tcgacaacac
     7261 cccgtcgccc accctggaca tcccggcccg ctatcgcgac caccagttca cccaggaatt
     7321 ccagctgctg ctggaaggcg agaagtggaa cggcgtcgcc ggcctgttct acctcaacgc
     7381 caccgcctcg ggcgcgttcg acaccgtgct gggcctggcc aacctgacca tcgccacggc
     7441 cggcgaggtc aacacccgca gctacgccgc cttcgccgac ttcagctacg acttcaccga
     7501 ccggttctcg gtgtcggtcg gcggccgcct gacgcgcgac gtcaaggacg gctacgtcta
     7561 ccgccagaac ttcaccggca tccgcagccc gctgttcggc aacaccgcgg cgatcccggg
     7621 cctgctgcgc accgacccgg ccctctacgc cggcaagcag ctggactgga aggagttcac
     7681 tccgcgggtc agcgcctcct acaagctgac gcccgacctg accgcctacg cctcctgggg
     7741 caagggcttc aagtccggcg gcttcgacat gcgcggcgac tacgtctcct acccgggcac
     7801 ggtggacggc tatgatccgg aattcgtctc gaccaccgaa ctgggcctca agggcgcgct
     7861 gttcggcaat cgcctgaact tcgccacggc gatcttcgac tcgagctacg aagaccagca
     7921 gatcaccacg cagtatccct cgggcctgtc ggtggcgagc gtggtcgaca acgtcgggtc
     7981 gagctcgatc cagggctggg aattcgaagg gtcggcgcga ctgttcgacg gcttcacggc
     8041 caacctgatg ctcagctaca tcgacgcctc gttcgacaag tacatcgcct tcattcccgg
     8101 cacgggctac gtcgacgtgt cgaacgcgcg cgacttccag aacaccccgg actggagcgg
     8161 ctcgggcggg ttcacctacg tccatgacct gggcgaccac ggcacgctga ccttcacccc
     8221 gaccgcctcc tatcgtggcg actcgcagat gttcgaggtc gccagcccgg tcctcgacca
     8281 gcccgcctac tggctctacg acgccagcct ggtctgggcg tcgcccgacg gccagtacaa
     8341 ggtcggcctg catggcaaga acctcggcga cgaggagtac cgggtcgggg gctacaactt
     8401 cccgggcgcg ctcttcggcg actcgatcat cggctactac ggtccgccgc ggaccgtgac
     8461 gctgaccttc gacgcccgct tctgatctga tcctccctga acggcggcga tccagccggg
     8521 tcgccgccgt cttcttttgc agctagggat caggcatgaa caccgggtcg aaggacgcca
     8581 cccccgcggg cggctatcgc tactacgtgc tggcgatgct catcgtcgtc tacacgttca
     8641 acttcctcga ccggcagatc ctcgggatcc tcgccacccc gatcaaggcg gagttcggcc
     8701 tcagcgacgg ccagttcggc ctgatgagcg gcctggcctt cgcgttgctc tacaccaccc
     8761 ttggcgtgcc gatcgccctg ttggccgacc gcttcagccg gtcgtggatc atgaccgccg
     8821 ccttcgccct gtggagcggc ttcaccgccc tgtgcggcgt ggtcacctcg ttcccgatgc
     8881 tgttcctgat gcgcatgggc gtgggcatcg gcgaggccgg gggcaccgcg ccggcctatt
     8941 cgctgatcgc cgactacttc ccgcccagcc agaggggccg ggccctggcc gccttcgcct
     9001 tcggcattcc cgccggcatg ggcgcgggca ccctgatcgg aggcctggtc gccgcccagt
     9061 acggctggcg catgtcgttc atcgtcgtcg gcctcgccgg cctggccctg gccatcccgt
     9121 tcaagctgct ggtccgcgac ccgccccgcg gcggccagga ctccccgccc gcgccggtgg
     9181 cggcggcgcc gttcggcaag gtgctcggcg ctgtcctgcc caagcccagc ttctggctgc
     9241 tgtcgttcgg ggccgcctcg tcatcggtct gcggctacgg cgtcgcggcc tggctgccgt
     9301 ccttcttcca gcgcagcttc ggcctgaccc tggtcgagac ctcgtggtac gcggcgggca
     9361 tctcgatcct cggcgggctc ctgggcatct ggcttggcgg agcggtggcc gaccggctcg
     9421 gcgcgacgtc gaagcgcgcc tatccgctga cgccggcggt cgccttcctg gtcgccgtgc
     9481 ccctgttcct ggcggcgatg aacagcggac cgctgctggc gggcctcggc ggcggaggat
     9541 cgcggctcgc gtcgctggcg ctgggcttcg gcctgttcct cattcccacg ggcctgaacc
     9601 tcgcctggat cggaccgatc accgcgacgg tgcagcacct tgtcccgccg cacatgcggg
     9661 ccacggcctc ggccctgttc ctgctgatca acaacctgct cgggatcgcc ggcggcatct
     9721 actatttcgg cttcatgtcc gacctgctgg cgccgcactt cgggacggaa tcgctgcgct
     9781 ggtcgatcta caccggcatg ggcttctacc tgctgtcctc ggccctgttc gtcctggcgt
     9841 cgcgcgtgct ggagaaggac tggcaagact gacgcggaat cggccgccga tcggggctca
     9901 acgcaccccg tatcgcccgg catcgcagca tgttcttcct gatgccgtcc atcgggttgc
     9961 gcccgccgcg gcccgccgac tataacccgc gcgacccgcg cccgcgggcc tttcccgcat
    10021 ccgcgagacc cacagagcgc gatgaatatt cacgagcatc aggccaaggc cgtcctggcc
    10081 gaattcggcg tccccgttcc gcgcggcttc gccgccttta ctcccgatga agccgccacg
    10141 gccgccgaga cgctcggcac gcccgtcttc gtcgtgaaga gccagatcca cgccggcggg
    10201 cgcggcaagg gcaagttcga agggctcggc ccggacgcca agggcggcgt ccgcgtggtc
    10261 aagtcggtcg acgaggttcg cagcaacgcc gccgagatgc tcggccgggt gctggtgacc
    10321 caccagaccg gtcccaaggg caagcaggtc aaccgcctct acatcgagga aggcgcggcc
    10381 atcgccaagg aattctacct ctcgctgctg gtcgaccgcg cgtccagcca tgtgtcggtt
    10441 gtcgcctcga ccgagggcgg catggacatc gaggacgtgg cgcacaacac gcccgacaag
    10501 atccacacct tcaccatcga cccggcgacg ggcgtgtggc cgacccacca gcgcgccctg
    10561 gccaaggcgc tcggcctgac cggcggcctg gccaaggaag cggcctcgct gctgacccag
    10621 ctctatacgg cgttcctggc caaggacatg gccatgctgg agatcaaccc gctgatcgtc
    10681 accgccgacg accacctgcg ggtcctcgac gccaagctgt cgttcgacgg caacagcctg
    10741 ttccgccacc cggacatcaa ggccctgcgc gacgagaccg aagaagaccc caaggaaatc
    10801 gaagccagca agttcgacct cgcctacatc gccctcgacg gcgagatcgg ctgcatggtc
    10861 aacggcgccg gcctggccat ggccaccatg gacatcatca agctgtacgg cgccgagc
//
LOCUS       DAORNX010000042        10791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4571_length_10791_cov_6.538469, whole genome shotgun sequence.
ACCESSION   DAORNX010000042 DAORNX010000000
VERSION     DAORNX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_10791_cov_6.538469"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /locus_tag="PLO65_03150"
     CDS             complement(<1..2248)
                     /locus_tag="PLO65_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRD27278.1"
                     /translation="MADLKHVAKADGRLDLTGAPEGFDALVMADIARARGGLTMFVAR
                     DGGRASAFIDAMRFFAPELPVLQFPSWDCLPYDRTGPSAGIAAQRMATLSTLARGEDS
                     GKASLLVVSAPAILQRVPSRPDLARAGYSARPGQDVAIADLERYFAVNGYSRASTVSE
                     RGEFAIRGGVIDVFPPGAEEPVRLDLFGDTLESIRAFDPETQRSTRQLKEIDLLPVSE
                     VLLDPDAIARFRKGYVAAFGAPGEDALYDTVSEGGRRAGMEHWLPLYYERLETLFDYL
                     PPHTLIGLDHLVGEAEAERLAMVADAYDARASADRKSAYRPLPPEALYLTAEEVAQRI
                     AHRPNRWFTPFQSDMPGAVDMGAKLGRTFAAERAQDSVNLFQATADHATRQSAAGKRV
                     LFASWTEGSSERLGTMLADHGLKRVPFAPYWQAAKAADPRTPQRVVLPLESGFETDNL
                     AVISETDILGDRLARPRRKRRAANFLAEASALTQGDLVVHIDHGIGRYAGLKTLEVQG
                     APHDCLELHYAGEAKLYLPVENIDLLTRYGADGENIQLDKLGGAGWQSRKAKAKQRLR
                     EMAEGLIQIAAARQLKSVEETDPPSGLFDEFCARFPYEETDDQLNAIGDVLSDLASGK
                     PMDRLICGDVGFGKTEVALRAAFVMAMSGKQVAIVCPTTLLARQHFKTFSERFQGWPM
                     KVRRLSRLVPAREAAETREGLAAGEIEIVVGTHAVLSKQVSFKDLGLVIVDEEQHFGV
                     KHKEKLKEL"
     gene            complement(2241..2567)
                     /locus_tag="PLO65_03155"
     CDS             complement(2241..2567)
                     /locus_tag="PLO65_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRD27279.1"
                     /translation="MSKDALAHPSHPLYIRRLCMDEHRLKKIRLRAWRRGFREADLIL
                     GPFADRHCPTMTAAQLDAFEALLEEADQDIYDWYLGRSPVAAHINPEIFALLQAFDPS
                     GRPAGG"
     gene            2683..4755
                     /gene="recG"
                     /locus_tag="PLO65_03160"
     CDS             2683..4755
                     /gene="recG"
                     /locus_tag="PLO65_03160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRD27280.1"
                     /translation="MRPEILFPLFAPVSTLKGVGPKVEPLVERLAGPLVRDVLFLGPQ
                     ALIHRTPTTVDSVVDGQVHTLNVQVELHTKPMRMDQPWKIRTFDGTAFLTLIWFKGHG
                     EHLMRQHPVGARRVVSGKIERWGNEIQMAHPDYILPVERAAEIPETEAVYPATAGLPA
                     RTVRKLALEALARAPELPEWQDAAWKAREGFVGWRAALERLHNPQTDADLSPLASHRR
                     RLAYDELLAHQLALAQRKAARRAQPAPVIPAGGMADRIEAALPWKLTGAQIRALSEVR
                     GDLQSGEKMSRLLHGDVGAGKTIVAMLAMADAAEAHLQSVLMAPTEILARQHFETIGA
                     PLEAQGLRVVLLTGRDKGGVRTEKLMALMDGAAQVAVGTHALFQDDVRYRALGLTVID
                     EQHRFGVSERKRLQDKGESAHLLAMSATPIPRTLELTVFGDLDVSRIDEKPPGRTPVA
                     TRAVPSPRMGEIIVRLQEAVRGGAQAFWICPMVSESEFADLAAAEHRVVELRKVMGAG
                     VGLVHGQMAAAEKDAVMAEFAEGRLPVLVATTVVEVGVNVPNATIMVIEQAERFGLAQ
                     LHQLRGRVGRGRRESACVLLYDPPLSDTAQQRLDILRRTDDGFLIAEKDLELRGGGDP
                     LGLKQSGFPAYRLADPVAHRTMIAAAADDARLILGRDPALTSDRGQAVRVLQELFDWR
                     AGGLTDAG"
     gene            4776..5753
                     /locus_tag="PLO65_03165"
     CDS             4776..5753
                     /locus_tag="PLO65_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRD27281.1"
                     /translation="MSDLQSALTDLAPQLGGTRIDGLRRLSGGASQETWAFSLDTGRP
                     LILRRKPPGTAGVTGTQTPLATEAALIRAAARQGAPVPEVLAICDPGSDVGEAYVMAA
                     VEGETLGKRIARDDNFAAIRPKLAFQCGQALARIHAIPLDDSLPPLNRSSALDELEKY
                     ETVYRNLGARRPILEAAFRYLKDRAPPLDRPVMLHGDFRNGNIMFHPETGLAAVLDWE
                     LVHIGDPAEDMGWVCTASWRFGGNKPVGGFGDYADLLAGYEAGGGTPIPLERVLYWQM
                     LGSLKWGVMCEGMYASFANGTDPSVERAMIGRRTSECEIDLMALLERAA"
     gene            5750..6118
                     /locus_tag="PLO65_03170"
     CDS             5750..6118
                     /locus_tag="PLO65_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HRD27282.1"
                     /translation="MITHPTAPELIDSVIRFIEERAAPNLKDRDAFLCRVAVNALAAV
                     RREIEQGPAAETAALERLRALLGREGDFATLNAALCDGIADGTIDPLAPAVLAHMKAS
                     IIDQVRIDQPQYAGLKTLAS"
     gene            6223..6642
                     /locus_tag="PLO65_03175"
     CDS             6223..6642
                     /locus_tag="PLO65_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD27283.1"
                     /translation="MGSGSIESELLALEHRYWRSMKDRDGATATGLTDFPCIVSGASG
                     VASVDEETYLRLLAGGDWTINEFALGDGVVVRQLTDDVAIIAYRIRESLTVDGEAVTL
                     EAADASVWVRRDGRWRCAMHTETPTGDPYGRDRKAAG"
     gene            complement(6639..7883)
                     /locus_tag="PLO65_03180"
     CDS             complement(6639..7883)
                     /locus_tag="PLO65_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27284.1"
                     /translation="MARFALPGRLWGHADFMRLWAAQAISAFGARITREGLPLAAVLT
                     LDATPGQLGVLAALSMGPGLIVGLLAGGLVDRTSRRAILVGADLLRFTVLMTVPVAAW
                     LHLLGMTQLYVVAALVGAATVLFDLADRAFLPTIVATDDLMEANSRLSVTQSTAEIGG
                     PALAGLLFQLLTAPFAIAIDAVTYLVSALFLGGLRAREPVNREHRDAQPLAADLAFGW
                     RTVMADPRVRPLLFLFTLQMVFFAFFGSLYALFALKTLQLAPAALGLTIALGGVGALL
                     GAGLAPLLVRMLGVGPAIIASGVTAAAAFTLIPLAPADPVAGMAFLMASQLVGDAFGV
                     AAMIPTTTLQQTLVAPEALGRVGAIFQIGRGAGWIVGAALCGGLAAVVGVRETLAIAC
                     VGLLLAELLLLFSPLRTLRTLG"
     gene            complement(7887..9059)
                     /locus_tag="PLO65_03185"
     CDS             complement(7887..9059)
                     /locus_tag="PLO65_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRD27285.1"
                     /translation="MAGLKGFKRWIALGLVALAMVMALGAFVFRVDILRTSLDPKVPF
                     LAYSPPRAPDYAEARAWVLPIDPRPPAALPADVFFIHPTTYDGGRHWNAPFRDQEGAR
                     LLDRVMLPNYAGPFARVGRIFAPRYRQASLYSQLTLREDARAARAFAYGDVRRAFRYW
                     RAHHDTGRPLVLVGVEQGGLLAERLLREEIAPDPALSARLAGAYLIETVAPASSYDGA
                     ASVPACSRRDQARCVVAWATPRDGDRRRARDRALIWDPSGVLVELQGRAVLCVNPLTG
                     ARGDARADERLSLGAANATGMGWGVRPPLLKREVHTRCIDGFLDVSRPTSTAFRPARG
                     WADRLKVAPYNLFYRDLEVDAQARVSALLGRQAYGRPAPPISRSIPVQTSPVHRID"
     gene            complement(9129..10130)
                     /gene="rfaD"
                     /locus_tag="PLO65_03190"
     CDS             complement(9129..10130)
                     /gene="rfaD"
                     /locus_tag="PLO65_03190"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRD27286.1"
                     /translation="MTRRIVFVTGGAGFIGSNIIARLAEDPSLDLVACDWLGSADQGK
                     WRNLAKHPIGDFVAPEVMMDWLEKRWQDVAMVIHMGAISSTTEPDADRIIHQNFGLSR
                     DLFRWCTDRQRRLIYASSAATYGDGTQGFEDRDDIDSLKALRPLNTYGWSKALFDLFA
                     ARQAARDYAPPQWVGLKFFNVYGPNEEHKESMKSVAAQIWPHVREGRSVQLFKSHNPD
                     YEDGGQLRDFVYVRDVADVVQWLYRNEQVNGVYNLGSGKARSFRDMAQNVFKAAGREP
                     QIDYIPMPTSIRDKYQYFTEAKMDRLREAGYPGQMTPLEAGIGEYVGKFLNTADPYR"
     gene            complement(10134..>10791)
                     /gene="rfaE2"
                     /locus_tag="PLO65_03195"
     CDS             complement(10134..>10791)
                     /gene="rfaE2"
                     /locus_tag="PLO65_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRD27287.1"
                     /translation="SGAGDTALAALGLALAARSSHEDAVAFALLASGVVVGKAGTAVA
                     TPHELVEAELAAHLAPAEAKVVLPDEMASKAAAWRAQGFRIGFTNGCFDILHKGHVAY
                     LAQARTWCDRLIVGVNSDRSVRALKGEGRPVNDLESRALVLAGLGSVDLVVPFDEDTP
                     VELIRAARPDVLVKGADYTVEGVVGHEMVAAWGGEVKLAPIVEGYSTTAAIARMIEKA
                     "
BASE COUNT         1680 a         3752 c         3846 g         1513 t
ORIGIN      
        1 gaagctcctt gagcttctcc ttgtgcttga ccccgaagtg ctgctcctcg tcgacaatca
       61 ccaggcccag gtccttgaag ctgacctgct tgctgagcac cgcatgggtg ccgacgacga
      121 tctcgatctc gccggcggcg aggccctcgc gcgtctccgc ggcttccctg gccgggacca
      181 gccgcgacag ccggcgaacc ttcatcggcc agccctggaa ccgctcgctg aaggtcttga
      241 agtgctgccg cgccagcagg gtggtcgggc agacgatggc cacctgcttg ccgctcatgg
      301 ccatgacgaa ggccgcgcgg agggccacct cggtcttgcc gaagccgaca tcgccgcaga
      361 tcaaccggtc catcggcttg ccgctggcca gatcggacag cacgtcgccg atggcgttga
      421 gctggtcgtc cgtctcctcg taggggaagc gggcgcagaa ctcgtcgaac aggccggacg
      481 gcggatcggt ctcctcgacc gacttcagtt gccgggcggc ggcgatctgg atcagaccct
      541 cggccatctc gcgcagccgc tgtttagcct tggccttgcg gctctgccag ccggccccgc
      601 ccagcttgtc gagctggatg ttctcgccgt cggcgccata gcgcgtcagc aggtcgatat
      661 tctcgaccgg caggtagagc ttggcctcgc cggcgtagtg cagctccagg cagtcgtgcg
      721 gcgcgccctg cacctcgagc gtcttgaggc ccgcgtaacg gccgatgcca tggtcgatgt
      781 gcacgaccag gtcgccctgg gtcagggcgc tggcctcggc gaggaagttg gccgcccggc
      841 gcttcctgcg cggccgcgcg aggcggtcgc cgaggatgtc ggtctcggag atgaccgcga
      901 ggttgtctgt ctcgaagccg gactccaggg gcagcacgac gcgctgcggc gtcctcgggt
      961 cggccgcctt ggccgcctgc cagtaggggg cgaacggcac gcgtttgagg ccgtggtccg
     1021 ccagcatggt tccgagccgc tcggaggagc cctcggtcca ggaggcgaac agcacgcgct
     1081 tgccggccgc cgactgccgc gtcgcgtggt cggccgtggc ctggaacagg ttgacgctgt
     1141 cctgcgcccg ctcggcggcg aaggtgcgac ccagcttggc gcccatgtcg acggcgccgg
     1201 gcatgtcgct ctggaacggc gtgaaccagc ggttgggccg gtgggcgatg cgctgggcga
     1261 cttcctcggc ggtcagatac agcgcctcgg gcggcagcgg ccgataggcc gacttgcgat
     1321 cggcgctggc gcgggcgtca taggcgtcgg cgaccatggc cagccgctcg gcctcggcct
     1381 cgcccaccag gtggtcgagc ccgatcagcg tatggggcgg caggtagtcg aacagggtct
     1441 ccagccgctc gtagtagagc ggcagccaat gctccatgcc cgcgcggcgg ccgccctcgc
     1501 tgaccgtgtc gtagagggcg tcctcgcccg gcgcgccgaa ggccgcgaca tagcccttgc
     1561 ggaagcgcgc gatggcgtcg gggtcgagca gcacctcgct gacgggcagc aggtcgattt
     1621 ccttgagctg cctggtcgac cgctgggtct cgggatcgaa ggcgcggatc gactccagcg
     1681 tgtcgccgaa caggtccagc cgcaccggct cctcggcgcc gggcggaaag acgtcgatga
     1741 ccccgccgcg gatggcgaac tcgccgcgct cggacaccgt cgaggcgcgg ctgtagccgt
     1801 tgacggcgaa gtagcgctcc aggtcggcga tggccacgtc ctggccgggc cgggcgctgt
     1861 agccggctcg cgccaggtcg ggacgcgacg ggacgcgctg cagaatggca ggggccgaga
     1921 cgaccagcag cgaggccttg cccgagtcct cgccgcgcgc gagggtcgac agtgtggcca
     1981 tccgctgggc cgcgatcccg gccgaggggc cggtgcggtc atagggcagg cagtcccacg
     2041 acgggaactg cagcaccggc agttcgggcg cgaagaagcg catggcgtcg atgaaggcgc
     2101 tggcccggcc gccgtcgcgg gccacgaaca tggtcagacc gccgcgcgcc cgggcgatgt
     2161 cggccatcac caaggcgtcg aatccctccg gtgcgccggt cagatcaagg cggccgtcgg
     2221 ctttggcgac gtgtttgaga tcagccaccg gccggtcttc cggacggatc gaaggcctgc
     2281 agcagcgcga agatctcggg attgatgtgg gcggccaccg gcgatcgccc cagataccag
     2341 tcgtagatgt cctggtcggc ctcttccagc agcgcctcga aggcgtcgag ctgggccgcg
     2401 gtcatggtcg ggcaatggcg atccgcgaac ggccccagaa tgaggtccgc ttcccggaat
     2461 ccacggcgcc aggctcgcag cctgatcttc ttgaggcgat gctcgtccat gcagagccgg
     2521 cggatataga gcgggtggct gggatgcgcc agtgcgtcct tcgacacggc gcttcgcgcc
     2581 tgctcagcat gacgtgcatt ttgcccacgt catggtgagc agcgctcgaa gagcgcgtgt
     2641 cgaaccacgc aagatgccag cgaccgcgct acactccctc cgatgcgccc cgagattctg
     2701 ttcccgctct tcgccccggt gagcacgctg aaaggcgtgg ggccgaaggt cgagccgctg
     2761 gtcgagcgac tggccggccc actggtgcgg gatgtgctgt tcctcgggcc gcaggcgctt
     2821 atccatcgca cgccgaccac ggtcgacagc gtggtcgacg gccaggtgca caccctcaat
     2881 gtccaggtcg aactgcatac caagccgatg cgcatggacc agccgtggaa gatccgcacc
     2941 ttcgacggca cggccttcct gacgctgatc tggttcaagg gccacggcga gcatctgatg
     3001 cgccagcatc cggtcggcgc acggcgcgtg gtcagcggca agatcgagcg ctggggcaac
     3061 gagatccaga tggcccatcc ggactacatc ctgccggtcg agcgggccgc ggagattccc
     3121 gagaccgagg ccgtctatcc ggcgaccgcc ggcctgcccg cccgcacggt gcgcaagctg
     3181 gcgctggaag ccctggcgcg cgcgcccgaa ctcccggaat ggcaggacgc cgcctggaag
     3241 gcccgcgagg gattcgtcgg ctggcgcgcg gcgctggagc gattgcacaa tccgcagacc
     3301 gacgccgacc tgtcgccgct agcctcgcac cggcggcggc tggcctatga cgagctgctc
     3361 gcgcaccagc tggcgctggc ccagcgcaag gccgcgcgcc gggcccagcc cgccccggtg
     3421 atcccggccg gggggatggc cgaccgcatc gaggccgccc tgccgtggaa gctgaccggc
     3481 gcccagatcc gcgccctgtc ggaggtgcgc ggcgatctgc agtccggtga gaagatgagc
     3541 cggttgctgc atggcgatgt cggcgcgggc aagacgatcg tcgccatgct ggccatggcc
     3601 gacgcggccg aggcgcatct gcagtccgtg ctgatggcgc cgaccgaaat tctcgcccgc
     3661 cagcatttcg agaccatcgg cgccccgctc gaggcccagg ggctgcgcgt ggtgctgctg
     3721 accggacgcg acaagggcgg cgtgcggacc gagaaactca tggcgctgat ggacggcgcg
     3781 gcccaggtcg cggtcggcac ccacgccctg ttccaggacg acgtccgtta ccgcgccctc
     3841 ggcctgacgg tgatcgacga gcagcaccgc ttcggcgtca gcgagcgcaa gcggctgcag
     3901 gacaagggcg aaagcgccca tctgctggcc atgtcggcca cgcccatccc gcgtacgctg
     3961 gagctgaccg tgttcggcga cctagacgtc agccggatcg acgagaagcc tcccggtcgc
     4021 acgccggtgg cgacccgcgc ggtcccctcc ccccgcatgg gcgagatcat cgtccggctg
     4081 caggaggccg tgcgcggcgg ggcccaggcc ttctggatct gcccgatggt cagcgagagc
     4141 gagttcgccg acctggccgc cgccgagcac cgcgtggtcg agctgcgcaa ggtcatgggc
     4201 gccggggtgg ggctggtcca cggccagatg gccgccgccg agaaggacgc ggtgatggcc
     4261 gagttcgccg agggccgcct gcccgtgctg gtcgcgacca cggtcgtcga ggtcggcgtc
     4321 aatgtgccca acgccaccat catggtcatc gaacaggccg agcggttcgg cctggcccag
     4381 ctgcaccagc tgcgcggccg ggtcggccgg ggccggcgcg aaagcgcctg cgtgctgctc
     4441 tacgacccgc cgctgtccga caccgcccag caacggctcg acatcctgcg acgcaccgac
     4501 gatggcttcc tgatcgccga gaaggatctg gagctgcgcg gcgggggcga cccgctcggc
     4561 ctgaagcaga gcggctttcc ggcctaccgc ctggccgatc cggtggccca caggacgatg
     4621 atcgccgcgg cggcggacga cgcccggctg atcctcggtc gcgaccccgc cctgacctct
     4681 gatcgcggcc aggccgtgcg cgtgctgcag gaactgttcg actggcgcgc cggcggtttg
     4741 actgacgccg ggtgaagggt ccccaatcca gccccatgag cgatctgcaa tccgccctga
     4801 ccgatctcgc gccacaactc ggcgggaccc gtatcgacgg cctgcgccgc ctgtccggcg
     4861 gggccagcca ggagacatgg gccttcagcc tcgacaccgg ccggccgctg atcctgcgcc
     4921 gcaagccgcc ggggacggcc ggcgtgaccg gaacccagac gccgctggcc accgaggcgg
     4981 cgctgatccg cgccgccgcc agacagggcg ctccggtccc ggaagtgctg gccatctgcg
     5041 atccgggaag cgacgtcggc gaggcctatg tgatggccgc ggtcgagggc gagacgctgg
     5101 gcaagcgcat cgcccgcgac gacaatttcg ccgccatccg cccgaagctg gccttccagt
     5161 gcggccaggc cctggcgcgg atccacgcga ttccgctcga tgacagcctg ccgccgctca
     5221 atcgctcgag cgcgctcgac gaactggaga agtacgagac ggtctatcgc aacctcggcg
     5281 cccggcgacc gatcctcgag gcggccttcc gctacctcaa ggaccgcgcg ccgccgctcg
     5341 accggccggt catgctgcat ggcgatttcc gcaacgggaa catcatgttc catcccgaaa
     5401 ccggactggc ggcggtgctt gactgggaac tggtccacat cggcgatccg gctgaggaca
     5461 tgggctgggt ctgcaccgcg tcctggcgtt tcggcgggaa caagcccgtg ggcgggttcg
     5521 gcgactatgc cgacctgctg gccggctacg aggccggcgg cgggacgccg atcccgctgg
     5581 agcgggtgct atattggcag atgctcggct ccctgaagtg gggcgtcatg tgcgaaggca
     5641 tgtacgcctc cttcgccaac ggaaccgatc cctcggtcga acgggcgatg atcggccggc
     5701 gcacctcgga atgcgagatc gacctgatgg ccctgctgga gcgcgcggca tgatcaccca
     5761 ccccaccgcg ccggagctga tcgactcggt gatccgcttc attgaggaac gggccgcgcc
     5821 caatctgaag gaccgcgacg ccttcctctg ccgggtcgcc gtcaacgccc tggcggcggt
     5881 caggcgagag atcgaacagg gtccggcggc cgagaccgct gcgctggagc gcctgcgcgc
     5941 gttgctgggc cgggagggcg acttcgccac cctcaacgcc gcgctctgcg acggcatcgc
     6001 cgacggcacg atcgatccgc tcgccccggc cgttctggcc cacatgaagg ccagcatcat
     6061 cgaccaggtg cgcatcgacc agccgcagta cgcgggactc aagaccctgg ccagctgacc
     6121 ctgcgcagga accggccgtc gtccgggacc gttcgagtcg tttcgccccg cgacccggcg
     6181 catcggcgcc gtacggtggc ggcgcgcagc ccggaggtcg atatgggaag cgggtccatc
     6241 gaaagcgaat tgctggcgct cgaacatcgc tactggcggt cgatgaagga cagggacggc
     6301 gcgaccgcca cgggcctgac ggactttccc tgcatcgtct ccggcgcttc gggcgtggcc
     6361 agcgtggacg aggaaaccta cctccggctc cttgcgggcg gcgactggac catcaacgaa
     6421 ttcgcccttg gcgatggcgt cgtggtgcgc cagctcaccg acgacgtggc gatcatcgcc
     6481 tacaggatcc gggaaagtct gaccgtcgat ggcgaggccg tgaccctgga ggccgccgac
     6541 gcctcggtct gggtgcgccg cgacggccgc tggcgatgcg ccatgcatac cgagaccccg
     6601 acgggcgacc cctatggccg cgaccggaag gcggccggct aacctaacgt ccgcaatgtt
     6661 ctgagcggcg agaacagcag aagcagttcg gccagcagca ggccgacgca ggcgatcgcc
     6721 agcgtctcgc gcacgccgac gaccgccgcc aggccgccgc acagggccgc gccgacgatc
     6781 catcccgcgc cgcggccgat ctggaagatg gccccgaccc gccccagcgc ctcgggcgcg
     6841 acaagggtct gttgcagggt ggtcgtcggg atcatcgccg ccacgccgaa cgcgtccccg
     6901 accagttgcg aggccatgag gaacgccatg ccggcgaccg gatcggcggg ggccagcggg
     6961 atcagcgtga aggcggcggc cgcggtgaca ccgctggcga tgatggccgg accgaccccg
     7021 agcatcctga caagaagggg cgccaggcct gcgcccagca gggccccgac gccgcccagg
     7081 gcgatggtca ggccgagggc cgcaggagcc agctgcagcg tcttgagggc gaacagggcg
     7141 tagaggctcc cgaagaaggc gaagaacacc atctgcagcg tgaacagaaa caacagcggc
     7201 cgcacgcgag ggtcggccat caccgtccgc cacccgaacg ccagatccgc cgcgaggggc
     7261 tgcgcatccc ggtgttcgcg gttcacgggc tcgcgcgccc gcaggccgcc caggaacagg
     7321 gccgagacga gataggtgac ggcgtcgatg gcgatggcga acggcgcggt cagcagctgg
     7381 aacagcaggc ccgccagcgc gggcccgccg atttcggcgg tcgactgcgt gacgctgagg
     7441 cggctgttgg cctccatcag gtcgtccgtc gcgacgatgg tcggcaggaa ggcccggtcg
     7501 gcgaggtcga acagcacggt cgccgccccg accagggccg cgacgacata gagctgggtc
     7561 atgcccagga ggtgcagcca ggcggcgacc ggcacggtca tcaggactgt gaaccgcagc
     7621 aggtcggcgc cgaccagaat ggcgcggcgg ctggtccggt cgacaagacc cccggccaga
     7681 agaccgacga tcagccccgg ccccatcgac agggcggcca gtacgccaag ctgccccggg
     7741 gtcgcgtcga gcgtcagcac cgccgccagg ggcaggcctt cgcgggtgat ccgcgcgcca
     7801 aaagccgata tggcctgggc ggcccacagc ctcatgaagt ccgcgtggcc ccagaggcgg
     7861 cccggaaggg cgaaccgggc cacggcctag tcgatcctgt ggaccggcga cgtctgcacc
     7921 gggatcgagc ggctgatcgg cggcgcgggg cggccatagg cctggcgacc caacagggct
     7981 gagacccggg cctgggcgtc gacctccagg tcccggtaga acaggttgta cggcgcgacc
     8041 ttcagccggt ccgcccagcc acgggccggt cggaaggctg tcgacgtcgg tctggagaca
     8101 tcaaggaagc cgtcgatgca gcgggtatgc acctcgcgct tgagcagggg cggtcgcacg
     8161 ccccagccca tgccggtcgc gttggccgcg cccaggctga ggcgctcgtc ggcccgggcg
     8221 tcgccgcgcg cgccggtcaa gggattgacg cacagcacgg cccggccctg cagttcaacc
     8281 agaacgccgc tgggatccca gatcagcgcc cggtcgcggg cccgacggcg gtcgccgtcc
     8341 cgcggcgtgg cccaggccac gacacagcgg gcctggtccc gtcggctgca ggccgggaca
     8401 gacgccgcgc cgtcgtagct gctcgcgggc gccaccgtct cgatcagata ggcgccggcc
     8461 agtcgcgcgc tcagcgcggg gtccggagcg atctcctcgc gcagcaatcg ctcggccaag
     8521 aggccgccct gctccacccc gaccagaacc agcggccgcc cggtgtcatg atgggcccgc
     8581 cagtagcgaa aggcgcggcg cacgtcgccg taggcaaagg cccgcgccgc ccgggcgtcc
     8641 tcgcgcaggg tcagctgcga atagaggctg gcctggcgat agcggggcgc gaagatccgc
     8701 cccacccggg caaagggtcc ggcgtagttg ggcaacatga cccggtcgag caggcgcgcg
     8761 ccttcctggt cgcggaacgg ggcgttccag tgcctgccgc cgtcataggt ggtcgggtgg
     8821 atgaagaaga cgtcagccgg caaggccgcc ggcggccgcg ggtcgatcgg caggacccag
     8881 gccctggcct cggcgtagtc gggggcccgg ggcgggctgt aggcgaggaa cggcaccttc
     8941 ggatcgagtg acgtgcgcag gatgtcgacg cggaacacga aggcgccgag ggccatcacc
     9001 atggccaggg cgaccagccc cagcgcaatc cagcgtttga atcccttcag gccagccatg
     9061 acccgagggt tacacgagga ccaggcggtc ggtaaacgcc cccgccgccg ggctggagcg
     9121 cgacccggtc accggtacgg gtcggcggtg ttgaggaact tgccgacgta ctcgccgatg
     9181 cccgcctcaa ggggcgtcat ctggcccgga taaccggcct cccgcaggcg atccatcttc
     9241 gcctcggtga agtactggta cttgtcgcgg atcgacgtcg gcatggggat gtagtcgatc
     9301 tgcggctcgc ggccggcggc cttgaacacg ttctgcgcca tgtcccggaa cgaacgcgcc
     9361 ttgccggagc ccaggttgta gacgccgttg acctgttcgt tccggtacag ccactggacg
     9421 acgtcggcca cgtcacggac atagacgaag tcccgcagct ggccgccgtc ctcgtagtcg
     9481 gggttgtgcg acttgaacag ctgcaccgac cggccctcgc ggacatgcgg ccagatctgc
     9541 gccgccaccg acttcatcga ctccttgtgc tcctcgttgg ggccgtagac gttgaagaac
     9601 ttcaagccga cccactgcgg cggagcgtag tcgcgggccg cctggcgcgc cgcgaacaga
     9661 tcgaaaaggg ccttggacca gccataggtg ttcagcggtc gcagcgcctt gaggctgtcg
     9721 atgtcgtcgc ggtcctcgaa gccctgggtc ccgtcgccgt aggtcgccgc cgaggaggcg
     9781 tagatgagcc ggcgctggcg gtcggtgcac cagcggaaca ggtcgcgcga cagaccgaag
     9841 ttctggtgga tgatcctgtc ggcgtcgggt tcggtggtcg agctgatggc ccccatgtgg
     9901 atcaccatcg ccacgtcctg ccagcgcttc tcgagccagt ccatcatgac ctcgggggcc
     9961 acgaagtcgc cgatcgggtg cttggcgaga ttgcgccact tgccctggtc ggccgagccc
    10021 agccagtcgc aggccaccag atccagcgac ggatcctcgg ccagcctggc gatgatgttg
    10081 gagccgatga agccggcccc gccggtgacg aaaacgattc ggcgggtcat gggtcaggcc
    10141 ttctcgatca tgcgggcgat ggcggcggtg gtggaatagc cctcgacgat gggcgcgagc
    10201 ttcacctcgc cgccccaggc cgcgaccatc tcgtggccga cgaccccctc gacggtgtag
    10261 tcggcgccct tgaccaggac gtcgggccgg gcggcccgga tcagctcgac gggcgtgtcc
    10321 tcgtcgaacg ggacgaccag gtcgaccgaa ccgagacccg ccagaaccag ggcgcgggat
    10381 tcgaggtcgt tgaccggccg gccctcgccc ttgagagcgc gcacagaccg gtcgctgttg
    10441 accccgacga tcagccggtc gcaccaggtc cgggcctggg ccaggtaggc gacatggccc
    10501 ttgtgcagga tgtcgaaaca gccgttggtg aagccgatcc tgaagccctg ggcccgccag
    10561 gcggcggcct tgctggccat ctcgtccggc aggaccacct tggcctcggc cggggccagg
    10621 tgggcggcaa gttcggcctc gaccagctcg tgcggcgtgg ccaccgcggt gccggccttg
    10681 ccgaccacca cgcccgaagc cagcagggcg aacgccacgg cgtcttcgtg cgacgaccgg
    10741 gccgccaggg cgagacccag cgccgccagg gccgtgtcgc ccgcgcccga g
//
LOCUS       DAORNX010000043        10677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4624_length_10677_cov_8.658633, whole genome shotgun sequence.
ACCESSION   DAORNX010000043 DAORNX010000000
VERSION     DAORNX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_10677_cov_8.658633"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>240)
                     /locus_tag="PLO65_03200"
     CDS             complement(<1..>240)
                     /locus_tag="PLO65_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27288.1"
                     /translation="QFVAADGSTATGVGAACGTGACVGGTTTCNAEGTGLGCTSLAQS
                     FVEVCDGVDNDCDGLTDSLDADLGRVDCDNQQGVC"
     assembly_gap    241..258
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<259..928)
                     /locus_tag="PLO65_03205"
     CDS             complement(<259..928)
                     /locus_tag="PLO65_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRD27289.1"
                     /translation="MLARAATPVVETVALAEADGRVLAEAITAGRDQPPFDASAMDGW
                     AVKRADVGHAVRLAIVGESAAGRGHLSPLQSGQTVRIFTGAPVPAGADLVVIQEDAER
                     DGDDDDACEDDVLTCISGLLACVDRGAPIDEICDGQDNDCDGLIDADDATLVLAACEN
                     QAGACVGATRTAEQCVEGLWASCTAQDYQRHSDQHPDPTLVGRFEADAETRCDGVDND
                     CDGES"
     gene            complement(978..1799)
                     /locus_tag="PLO65_03210"
     CDS             complement(978..1799)
                     /locus_tag="PLO65_03210"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRD27290.1"
                     /translation="MMTTAEAPAAPAKASPFFEETVLWVKHWTDRLFSFAITRPESFR
                     FRSGEFVMIGLPGEDGGKPVLRAYSIASPAWSDTLEFLSIKVQDGPLTSRLQHIQPGD
                     TVLMGKKPTGTLVLDAVKGGKRLFLFGTGTGLAPWLSVARDPDAFSRFEKVYVCHGVR
                     QVNELAYRDLFTREIHDDPLVGDEAKAQLTYYPTVTREPFEHQGRFPDLINSGQFFRD
                     LGIPEDRFNPAEDRVMLCGSMDFIKDMAAVMENQGLKEGSNAEPGDFVLERAFVG"
     gene            complement(1916..2074)
                     /locus_tag="PLO65_03215"
     CDS             complement(1916..2074)
                     /locus_tag="PLO65_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27291.1"
                     /translation="MPSRPRNHEVENENLKPSRDGQAPRPATEPDGAADSSRSPETET
                     DPGSGEQA"
     gene            2219..2294
                     /locus_tag="PLO65_03220"
     tRNA            2219..2294
                     /locus_tag="PLO65_03220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2252..2254,aa:Trp,seq:cca)
     gene            2356..2667
                     /gene="secE"
                     /locus_tag="PLO65_03225"
     CDS             2356..2667
                     /gene="secE"
                     /locus_tag="PLO65_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRD27292.1"
                     /translation="MAKKTGNLAAMKQRAAKAAAVVAAPAGQASTAPVAPRKPFNPVQ
                     FFREVRVEARKITWTSRKETWITSVMVFIMVLIAALFFFVVDWLLSGAVSLLLKLANA
                     G"
     gene            2672..3253
                     /gene="nusG"
                     /locus_tag="PLO65_03230"
     CDS             2672..3253
                     /gene="nusG"
                     /locus_tag="PLO65_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRD27293.1"
                     /translation="MTTQTAAEAIVKAPSNPHHKWYIVHAYSNFEKKVAEHIRDQARN
                     QGLEDCFSEILVPTEDVVEIRRGRKVNSERKFFPGYVLVKMDLTDEAYHLVKNTPKVT
                     GFLGSAAKPMPVSEKEVERIVGAIEEGVERPKPTISFEIGEQVRVTDGPFASFNGTVE
                     QVDEDRARLHVAVSIFGRATPVELEYNQVEKVA"
     gene            3457..3888
                     /gene="rplK"
                     /locus_tag="PLO65_03235"
     CDS             3457..3888
                     /gene="rplK"
                     /locus_tag="PLO65_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRD27294.1"
                     /translation="MAKKILGYIKLQVPAGSATPSPPIGPALGQRGVNIMGFCKEFNA
                     RTEKETKGTPLPTVITVYQDKSFTFITKTPPATHFIKQALGLKSGSNKPGRETAGKIT
                     RAQLREIAEGKMKDLNANDVEAAARIIEGSARSMGLQIVEG"
     gene            3892..4581
                     /gene="rplA"
                     /locus_tag="PLO65_03240"
     CDS             3892..4581
                     /gene="rplA"
                     /locus_tag="PLO65_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRD27295.1"
                     /translation="MAKQAKRIQAWTGDRDAIHSVEDAVKLVKANAKAKFDESIEISV
                     NLGVDPRHADQQVRGVVSLPSGTGRDVRVAVIAKDAKAAEAEAAGADVVGAEDLVERI
                     QGGFMDFDRVIATPDMMALVGRLGKVLGPRGLMPNPRVGTVTPNVGQAVKDAKGGAIE
                     FRVEKAGIVHAGIGKASFTDDALLANVKALVDALSKAKPSGAKGTYIKRIALSSTMGP
                     GFKVDTASVSV"
     gene            complement(4648..4920)
                     /locus_tag="PLO65_03245"
     CDS             complement(4648..4920)
                     /locus_tag="PLO65_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27296.1"
                     /translation="MKMAPFPGLSAPWTGVDVRRLRAFAPSPVQTPRGRHAQLLTIAA
                     ARRLRCVERASLGRFEDAAYWKAVAPVAVFKAVELRTGGDFGALPA"
     gene            complement(5081..5620)
                     /locus_tag="PLO65_03250"
     CDS             complement(5081..5620)
                     /locus_tag="PLO65_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRD27297.1"
                     /translation="MSRPLASLVIAVATALAACSGAAGKDQVAQPAKPVDLELYLGRW
                     YELARYDMVFERGCEGVTADYSLRPDGKVRVLNTCRKGAPDGPVKTAEGKARVVPDSD
                     GAKLKVAFFGPFWADYWVLDRADDYSWAIVGEPSRKYLWILSRDPTPGPESTAALVAR
                     VAALGYDTDRLHMTEQPPR"
     gene            5991..6506
                     /gene="rplJ"
                     /locus_tag="PLO65_03255"
     CDS             5991..6506
                     /gene="rplJ"
                     /locus_tag="PLO65_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRD27298.1"
                     /translation="MNRAQKAESIETLKGVFADAGAVVVTHYMGLTVAEMTDLRLRLR
                     KEGAQIKVVKNTLAQKALAGSVGEAGDALFTGPVAIAYAPDPVSAAKVVTQYAKDNDK
                     FTVVGGLMGKDVLDQNAVKALATLPSLDQLRATLIGLLQAPATKVAGVVQAPAAQLAR
                     VFSAYGAKEAA"
     gene            6552..6941
                     /gene="rplL"
                     /locus_tag="PLO65_03260"
     CDS             6552..6941
                     /gene="rplL"
                     /locus_tag="PLO65_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRD27299.1"
                     /translation="MSKLDKIVEDLSSLTVLEAAELSKLLEEKWGVSAAAPVAMAMPA
                     AGGAAAAPAEAAEEQTEFTVVLTAGGDKKINVIKEVRGVRPDLGLKEAKDLVEGAPQN
                     VVENVSKQAAEEVAKKLTEAGATVQIK"
     gene            7125..7916
                     /locus_tag="PLO65_03265"
     CDS             7125..7916
                     /locus_tag="PLO65_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRD27300.1"
                     /translation="MTSNPFGDLAVLRETPVPDLRAVRARLRDPRRPPPNPDAPQFGE
                     PLVNIEALGLSGASYYAGSRNPPYYHPAPGAVSEVLVRESVGRLLTGVNARLAPVGLE
                     VFLFDGWRPTAVQAYFHDQWLPGQIRRRLPDMTGQALRREVGKYWAAPSSDPAAPAPH
                     NTGGAVDLTLRWKDSGEQLWMGSLFDDASAVAGLDFMEKKLARGPVMAFSDEEARANR
                     RLLCHLMTGAGFALNPFEWWHYGYGERMWAQLGRRPFALYGPARP"
     gene            complement(7920..9851)
                     /locus_tag="PLO65_03270"
     CDS             complement(7920..9851)
                     /locus_tag="PLO65_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HRD27301.1"
                     /translation="MIRRTLTALALLGLAALPAHARQSAGPQPYPAVSDIPAAKDVPY
                     PGQLTLRVDATDLDRRIFRVKETIPVAGPGPMVLLYPQWLPGNHAPRGQLEKVAGLTI
                     TAAGKPVRWFRDPVEVYALHVEVPEGASALELEFQFLSATAGDQGRVVMTQEMLNLQW
                     NSTAFYPAGWFTRQIPVEASVTLPPGWKFGVALDVAAQAGDTTTFKPVSFETLVDSPM
                     FAGRYFKQVDLDPGGRSPVRLNIVADRPSLLEMTDEQIQKHRNLIVQADRLYGARHYD
                     RYDFLLALTERMGGIGLEHHRSSENQVEPEYFTDWDKMAPERNLLPHEYGHSWNGKFR
                     RGADLWTPDYRTPMRDSLLWVYEGQDQYWGYVLGARSGLVSKQDTLDALAVTAATFDA
                     RVGRQWRPLEDTTNDPIISARRPKGWLSWQRNEDYYSEGQLIWLDVDTLIRERTGGKK
                     SLDDFAKGFFGVDDGSWTPLAYRFDDVVAALNAVTPYDWAGFLRTRLEGHDAAPLDGL
                     ARGGYRLVWKDRPNAFARASESLYKRTDLTWSVGLLLNATGAITGVQWDGPAYKAGLT
                     VGTQLVAVNGESYSADRLKDAITAAKGGTTPIELIVKTGEQYRVVRIARTGGLRFPHL
                     ERIEGTPDRLGDILAARRK"
     gene            9961..10119
                     /locus_tag="PLO65_03275"
     CDS             9961..10119
                     /locus_tag="PLO65_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27302.1"
                     /translation="MLKLAGVFLVIMIIAGVLGFVVDVAGFAAKVAFFVCLVGLVGSL
                     AARGLNRR"
     gene            10401..>10677
                     /locus_tag="PLO65_03280"
     CDS             10401..>10677
                     /locus_tag="PLO65_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27303.1"
                     /translation="MAQSFTGKKRIRKSFGRIPEAVQMPNLIEVQRSSYEQFLQREVR
                     SGERRDEGIEAVFKSVFPIKDFNERAVLEYVSYEFEEPKYDVEECIQR"
BASE COUNT         1729 a         3648 c         3579 g         1703 t
ORIGIN      
        1 gacagacgcc ctgctggttg tcgcagtcga cacggccgag gtccgcgtcg agcgagtccg
       61 tgagcccgtc gcagtcattg tcgaccccgt cgcagacctc gacaaacgac tgcgcgagcg
      121 aggtacagcc aagccccgtg ccctccgcgt tgcacgtcgt cgttcccccc acacacgcgc
      181 ccgtgccgca cgccgcaccc actcccgtcg cggtcgagcc gtcggcggcc acgaactgga
      241 nnnnnnnnnn nnnnnnnncg gactccccgt cgcagtcgtt gtcgaccccg tcgcagcgcg
      301 tctccgcgtc ggcctcgaag cgccccacga gggtcgggtc gggatgttgg tccgagtgcc
      361 tctgatagtc ctgcgccgtg cacgaggccc agaggccctc gacacactgt tcggcggtcc
      421 gggtggcgcc gacacaggcc cccgcctggt tctcacaggc cgcgaggacg agcgtcgcgt
      481 cgtccgcgtc gatgagcccg tcgcagtcgt tgtcctgtcc gtcgcagatc tcgtcgatcg
      541 gcgcgccgcg atcgacacac gccaacaagc ccgagatgca ggtcaggacg tcgtcctcac
      601 acgcgtcgtc atcgtcgcca tcgcgttcgg catcttcctg gatcaccacc aggtcagcgc
      661 cagccgggac cggggcgccg gtgaagatgc ggaccgtctg gccggactgc aggggggaca
      721 ggtggccgcg tccggccgcg ctttcaccga cgatggccag gcggacggcg tggccgacat
      781 cggcgcgctt gaccgcccag ccgtccatcg ccgaggcgtc gaacggcggc tggtcgcggc
      841 cggcggtaat cgcctcggcc agcacacggc catcggcctc ggccaaggcg actgtctcga
      901 ccacaggggt tgcggcgcgc gcgagcatcc gcgcccgggc atcctcgacg cccaggtttc
      961 gcggaccgcg atggggatca gccgacaaag gcccgttcga gcacgaagtc gcccggctcg
     1021 gcgttggagc cttccttcag gccctggttt tccatcacgg cggccatgtc cttgatgaag
     1081 tccatgctgc cgcagagcat gacccggtct tccgccggat tgaaccggtc ctcggggatg
     1141 ccgagatcgc ggaagaactg gccggagttg atcaggtcgg gaaagcggcc ctggtgctcg
     1201 aacggctcgc gggtgacggt cggatagtag gtcagctggg ccttcgcctc gtcgccgacc
     1261 agcggatcgt cgtgaatctc gcgggtgaac aggtcccggt aggccagctc gttcacctgg
     1321 cgcaccccgt gacagacata gaccttctcg aagcgcgaga aggcgtccgg atcgcgggcc
     1381 accgacaacc agggcgccag gcccgttccg gtgccgaaca ggaacagccg cttgccaccc
     1441 ttgaccgcgt ccagcaccag ggtgccggtc ggcttcttgc ccatcagcac cgtgtctccc
     1501 ggctggatat gctgcaggcg cgaggtgagc gggccgtcct ggaccttgat cgacaggaat
     1561 tccagcgtgt cggaccatgc cgggctggcg atcgaatagg cgcgcaggac cggcttgccg
     1621 ccgtcctcgc ccggcaggcc gatcatcacg aattcgccag agcggaaacg gaagctctcg
     1681 ggccgggtga tggcgaagct gaacagacgg tcggtccagt gcttcaccca cagcacggtt
     1741 tcttcgaaga aggggctcgc cttggcgggc gccgccgggg cctcggccgt cgtcatcata
     1801 actgcattcc tgaaggcccg cggggccgcc gcctcctcca tgtgatgcgg cgcagcatga
     1861 tgtcaacgtg aggaagtctc gcccgggagt caggatgtct gcgtcgaccc cggcgtcacg
     1921 cctgctcgcc cgatccggga tcggtctcgg tctccggcga gcgcgacgag tcggcggcgc
     1981 cgtcgggctc ggtcgccgga cgcggggcct ggccgtcccg cgacggcttg aggttctcgt
     2041 tctcgacctc gtggttcctg ggtcttgagg gcatcgtcga ctccggtcgg gtccgggaaa
     2101 cgcgccgacc gccgctttgc tccgcttgcc ccgaaatgcg ccgcctgcta catcgcgcgg
     2161 cttccggttg gccgggcgcg ccgcagggga gtccctgggg cgttcttggc gttcctggag
     2221 gagtgtagct cagctggtag agcatcggtc tccaaaaccg agggccgggg gttcgagtcc
     2281 ctccactcct gccaccctat atacggggcg cgatggtcgc gctccggcca gttgagagag
     2341 tttcagaccc gcccaatggc caagaaaacg ggcaatctcg ccgcgatgaa acagcgcgcc
     2401 gccaaggccg ccgccgttgt cgccgcgccg gctggacagg cgtcgacggc tcccgtcgcg
     2461 cccaggaagc ccttcaatcc ggtgcagttc tttcgtgagg tccgcgtcga ggcccgcaag
     2521 atcacctgga ccagccgcaa ggagacctgg atcacctcgg tgatggtctt catcatggtc
     2581 ctgatcgcgg cgctgttctt cttcgtcgtg gactggctgc tcagcggcgc cgtctcgctg
     2641 ctcctcaaac tcgcgaatgc gggataattc catgaccacg cagaccgccg cagaggccat
     2701 cgtcaaggcc ccgtccaatc cgcaccacaa gtggtacatc gtccacgcct actcgaactt
     2761 cgagaagaag gtggccgagc acatccgcga ccaggccagg aaccagggcc tggaggattg
     2821 tttctcggaa atcctcgtgc cgaccgagga cgtggtggag atccgccgcg gccggaaggt
     2881 caattccgag cgcaagttct tccccggcta cgtgctggtg aagatggacc tgaccgacga
     2941 ggcctatcac ctggtcaaga acaccccgaa ggtgacgggc ttcctgggca gcgcggccaa
     3001 gcccatgccc gtatcggaaa aggaagtcga gcgcatcgtc ggcgccatcg aggaaggcgt
     3061 cgagcggccc aagcccacga tcagcttcga gatcggcgag caggtccgcg tcaccgacgg
     3121 ccccttcgcc agcttcaacg gcactgtcga gcaggtcgac gaggatcgtg cgcgcctgca
     3181 cgtggccgtc tcgatcttcg gacgcgcgac ccccgtcgag ctggaataca accaggtcga
     3241 aaaggtcgcc tgattaccgg ggacatgtac cgcaggtacg tgtcccccaa tcgggatgcg
     3301 gcgggtttag gggacacgta cccttgcggg tacatgtccc cgtctgctac acgccgccgc
     3361 ttcctcagct tcggttgagg atcaaatccg tgggaggggc tgcccgcacc acggccgcat
     3421 aggcgctttg actagcgccg cttaacggag acagagatgg ccaagaagat cttgggctac
     3481 atcaagctgc aggtgcccgc gggctccgcc acaccctccc cgccgatcgg tccggcgctg
     3541 ggtcagcgcg gcgtcaacat catgggcttc tgcaaggagt tcaacgcccg caccgagaag
     3601 gaaaccaagg gcacgcccct gccgacggtg atcaccgtct accaggacaa gtccttcacc
     3661 ttcatcacca agaccccgcc cgcgacccac ttcatcaagc aggccctggg cctgaaatcc
     3721 ggctccaaca agccgggccg tgaaaccgcc ggcaagatca cccgcgccca gctgcgcgag
     3781 atcgccgaag gcaagatgaa ggacctcaac gccaacgacg ttgaagccgc cgcccgcatc
     3841 atcgagggtt ccgcccgctc gatgggcctg cagatcgtgg agggctagac catggccaag
     3901 caagccaagc gcattcaggc ctggaccggc gatcgcgatg cgatccactc ggtcgaggac
     3961 gccgtcaagc tggtcaaggc caacgccaag gccaagttcg acgagtcgat cgaaatctcc
     4021 gtcaacctgg gcgtcgaccc ccgtcacgcc gaccagcagg tccgcggcgt ggtcagcctg
     4081 ccctcgggca ccggccgcga cgtccgcgtc gccgtcatcg ccaaggacgc caaggccgcc
     4141 gaggccgagg ccgccggggc cgacgtggtc ggggccgaag acctggtgga gcgtatccag
     4201 ggcggcttca tggacttcga ccgcgtcatc gccacccccg acatgatggc cctcgtcggc
     4261 cgtctgggta aggtgctggg cccgcgcggc ctgatgccca atccgcgcgt cggcacggtc
     4321 acgccgaacg tcggtcaggc cgtcaaggac gccaagggcg gcgccatcga gttccgcgtc
     4381 gagaaggcgg gcatcgtgca cgccggcatc ggcaaggcct cgttcaccga cgacgccctg
     4441 ctggccaacg tcaaggctct ggtggacgcc ctgtccaagg ccaagccgtc gggcgccaag
     4501 ggcacctaca tcaagcgcat cgcgctgagc tcgaccatgg gtccgggctt caaggtcgat
     4561 accgcttcgg tcagcgtcta ggcgccagac cctctgcgag acaacggacg ggcgggctcg
     4621 caagggcccg cccgttctgc gttcgggtca ggcgggaagg gcgccaaagt cgccgcctgt
     4681 ccgcagctcc accgccttga acaccgcgac cggggccacg gccttccagt aggcggcgtc
     4741 ttcgaagcgg cccaggctcg cacgctcgac gcagcgtagc cggcgggcgg cggcgatggt
     4801 cagcagctgg gcatggcggc cgcgcggggt ctggacgggc gagggcgcga aggcgcgcag
     4861 gcggcgcaca tcgacgccgg tccagggggc ggagaggccc ggaaagggag ccatcttcat
     4921 cagtcggact ttcggcggcg gcggggcgct gcgttcggcg cgcccgcatc cggcgtctga
     4981 tcggcttcgc gatcctgatc cccaaatggg gacaacctgt ggatagttcc acgggaaggc
     5041 aaaattctgt caggattcca gaagtttatg acgaacgagg ttatctcggc ggctgttccg
     5101 tcatgtgcag ccggtcggtg tcgtagccga gggctgccac gcgggcgacc agcgcggcgg
     5161 tcgactccgg gccgggcgtg gggtcgcggc tgagaatcca caggtacttg cgcgagggct
     5221 cgccgacgat ggcccagctg tagtcgtcgg cgcggtcgag cacccagtag tcggcccaga
     5281 aggggccgaa gaaggccacc ttcagcttgg cgccatcgct gtccggcacg accctggcct
     5341 tgccctcggc cgtcttgacc ggcccgtcgg gcgcgccctt gcggcaggtg ttgagcaccc
     5401 ggaccttgcc gtccgggcgc agcgaatagt cggcggtgac gccctcgcag ccgcgctcga
     5461 agaccatgtc gtagcgcgcc agttcgtacc agcgtcccag atagagctcc aggtccaccg
     5521 gcttggccgg ctgcgcaacc tgatcctttc ccgcggcgcc ggaacaggcc gccagcgcgg
     5581 tggcgacggc gatcacgagg ctggcgaggg ggcgggacat ggcggttcct tcggtacggg
     5641 gcggaacggt ctacgcaagc gcggctctcg cggatcggct ccggcttgag tttcgcgccg
     5701 ccttcctcta tacgcgccca cttccgcgac cgggtccgcc cgggagcgac ctgtccgaga
     5761 accgcgcggc gatggggcca ccatcgtcgt aaccgaaggg gagcccccct tcagcgcggg
     5821 gaggcgggga tagagacgcc atcagcctcc gccctccggg cgcggagcgc ttcggctgaa
     5881 cttccttggg acaggcctac cgtaacccgc tttcgggagc gatcccgcgc gcgggttgca
     5941 cgctgatcgg cgtccggatg gtccgggcgt cgttgatcca ggagaccgca atgaaccgcg
     6001 cacaaaaagc ggaatcgatc gagacgctca agggcgtctt cgccgatgcc ggcgccgtgg
     6061 tcgtgaccca ctacatgggt ctcaccgttg cggaaatgac cgacctgcgc ctgcgcctcc
     6121 gcaaggaggg cgcgcagatc aaggtggtga agaacaccct ggcccagaag gccctggccg
     6181 gttcggtcgg tgaagcgggc gacgccctgt tcaccggtcc ggtcgccatc gcctatgcgc
     6241 cggatcccgt ctccgccgcc aaggtcgtca cccagtacgc caaggacaat gacaagttca
     6301 ctgtcgtcgg cggcctcatg ggtaaggacg tgctcgacca gaacgccgtc aaggcgctgg
     6361 ccaccctgcc gtccctcgac cagctccggg ctacgctcat cgggcttctg caagccccgg
     6421 ccaccaaggt ggcgggtgtc gtgcaggctc cggcggctca gctcgcccgc gtcttcagcg
     6481 cctacggcgc caaggaagcg gcctgatccc ggtcatctcc gcctatctct gaaccccaag
     6541 gaaactgaca catgtcgaag ctcgataaga tcgttgagga cctgtcctcc ctgaccgtcc
     6601 tggaagccgc tgaactctcc aagctgctcg aagagaagtg gggcgtctcc gccgccgctc
     6661 cggtcgccat ggccatgccc gccgccggcg gcgccgccgc tgctccggcc gaagccgccg
     6721 aagagcaaac cgagttcacc gttgttctga ccgccggtgg cgacaagaag atcaacgtga
     6781 tcaaggaagt ccgcggcgtc cgtccggacc tcggcctgaa ggaagccaag gacctcgtcg
     6841 aaggcgctcc gcagaacgtc gtcgagaacg tctcgaagca ggccgccgag gaagtcgcca
     6901 agaagctgac cgaagccggc gcgaccgtcc agatcaagta gtttgctgga cgcgcgcctc
     6961 agcgcgtccg cgaactaccc ccggaccggt tccggggtcc atggccaccc cggtgggcat
     7021 gggccctcgg gacaggcccg cggatgacgg accagaatga gcgggcccgg agggaaacct
     7081 ccgggcccgt tttctttgtg gcccgcccgg ttcagactgc atccatgacc agcaatccct
     7141 tcggtgacct ggcggtcctg cgggagacgc ccgtgccgga cctgcgagcg gtgcgcgcgc
     7201 gactgcgcga ccctcgtcgg cctccgccca acccggacgc cccgcagttc ggcgagccgc
     7261 tggtcaatat cgaggcgctc ggcctcagcg gggccagcta ctatgccggc tcacgcaatc
     7321 ctccctacta tcacccggct ccaggggcgg tttccgaggt cctggttcgc gagagcgtcg
     7381 gccggctgct gacgggggtg aacgcccgcc tcgcgcccgt tggcctggag gtcttcctct
     7441 tcgatggctg gcggccgacc gcggtgcagg cctacttcca tgaccagtgg ctgcccggcc
     7501 agatacgtcg acgcctgccc gacatgaccg gccaggcgct gcggcgggag gtcggaaaat
     7561 actgggccgc cccctcgagt gatccggccg cgcccgcgcc gcacaacacc ggcggcgccg
     7621 tcgaccttac cctgcggtgg aaggactccg gtgaacagct ctggatgggt tcgctgttcg
     7681 acgacgccag cgccgtggcc ggcctcgact tcatggagaa gaagctcgcg cgaggcccgg
     7741 tcatggcctt ttcggacgag gaggcgcgcg ccaatcgccg cctgctctgc catctgatga
     7801 ccggggccgg cttcgcgctc aatccgttcg agtggtggca ctacggctac ggcgaacgga
     7861 tgtgggcgca gctgggccgc cggccgttcg ccctgtacgg tccggcgcgg ccctgacggc
     7921 tacttcctgc gcgccgccag gatgtcgccg agccggtcgg gggtcccctc gatccgctcc
     7981 agatgcggga agcgcaggcc gccggtcctg gcgatgcgaa cgacccggta ctgttcgccg
     8041 gtcttgacga tcagctcgat cggcgtggtt ccgcccttcg ccgccgtgat ggcgtccttc
     8101 agccggtcgg cgctgtagct ctcgccgttg acggccacga gctgagtgcc gacggtcaga
     8161 cccgccttgt aggccggccc gtcccactgg acgccggtga tcgcgcccgt cgcgttcagc
     8221 agcaggccga cggaccaggt caggtccgtg cgcttgtaga ggctctcgct cgccctggcg
     8281 aaggcgttgg gcctgtcctt ccagaccagc cggtagccgc cgcgcgccag gccatcgagc
     8341 ggggccgcgt cgtgaccttc gagccgcgtc ctgaggaagc cggcccagtc gtatggcgtg
     8401 accgcattga gcgcggccac gacgtcgtcg aaccggtagg ccaggggggt ccaagagccg
     8461 tcgtcaacgc cgaagaagcc cttggcgaaa tcgtcgagtg acttcttccc gcccgtgcgc
     8521 tcgcggatca gggtgtcgac atcgagccag atcagctggc cctcggagta atagtcctcg
     8581 ttgcgctgcc agctgagcca gcccttgggc ctgcgggccg aaatgatcgg atcgttggtg
     8641 gtgtcctcca atggacgcca ctggcggccg acgcgggcat cgaacgtggc ggcggtcacg
     8701 gcgagggcgt cgagggtatc ctgcttgctg acaaggccgg accgcgcgcc gagcacatag
     8761 ccccagtact ggtcctggcc ctcgtagacc cacagcaggc tgtcgcgcat cggcgtgcgg
     8821 tagtcgggcg tccacaggtc ggcgccccgg cgaaacttgc cgttccagct gtggccgtac
     8881 tcgtggggca gcaggttgcg ctccggcgcc atcttgtccc agtcggtgaa atattccggc
     8941 tccacctggt tctccgacga acggtggtgc tccaggccga tcccgcccat gcgctcggtc
     9001 agcgccagca ggaagtcgta ccggtcgtag tggcgagccc cgtacagccg gtcggcctgg
     9061 acgatcaggt tgcggtgctt ctgaatctgt tcgtcggtca tctcgagcag cgacgggcgg
     9121 tcggcgacga tgttgagccg caccggcgag cggccgcccg gatcgagatc gacctgcttg
     9181 aagtagcgtc ccgcgaacat cggggaatcg accagggtct cgaagctgac cggcttgaag
     9241 gtcgtggtgt cgccggcttg ggcggcgaca tcgagcgcca cgccgaactt ccagcccggc
     9301 ggcagggtga cgctggcctc gacggggatc tgccgggtaa accagccggc cggatagaag
     9361 gccgtcgagt tccactgcag gttcagcatt tcctgggtca tcaccacccg gccctggtcg
     9421 ccggccgtgg ccgacaggaa ctggaactcc agctccagcg cggacgcccc ctcgggaacc
     9481 tcgacgtgca gggcgtagac ctcgaccggg tcgcggaacc accggaccgg cttgccggcg
     9541 gcggtgatcg tcaggccggc gaccttttcc agctggccgc gcggcgcgtg attgcccggc
     9601 agccattgcg gatagagcag caccatcggc ccgggcccgg cgacggggat cgtctccttc
     9661 acccggaaaa tgcgacggtc gaggtcggtg gcgtcgaccc gcagcgtcag ctggcccggg
     9721 taggggacgt ccttcgccgc cgggatgtcc gacacggcgg ggtagggctg cggccctgcg
     9781 ctctgccggg cgtgggcggg aagcgcggcc aggcccagca gggccagcgc tgtcagggtg
     9841 cgtctgatca cgggaaaact cgaagctgcc ggaggagcga tgaccccatt tgagccatcc
     9901 gggcgatgcg ccgcaacccc gcggaccgga acccgtgcgc gcgcggggcg ttgaccgccc
     9961 atgctcaaac tcgccggcgt cttcctcgtc atcatgatca tcgccggggt gctcggcttc
    10021 gttgtcgatg tcgctggctt cgccgccaag gtcgcctttt ttgtctgcct tgtgggactt
    10081 gtcggttcgc tggcggccag ggggttgaac cgacgctgag cgcccggcgg ccgggttgcg
    10141 aaagtctgaa gacgggcctt cccttccgtc cggctttccc ctatatccgc tccctcgcgg
    10201 aaaaaccgtt caactgagaa gcgatttttc gcgcgcgtag cccgatggcc cggtcatagc
    10261 cctccgaccg tggcgaaggg gcgctcctgt aacgccagga gcatcgtctt gtgtccgctt
    10321 ccggccatgc cgggagtcac gagggtggac gcgacaagac actttacgcg cggagtcccg
    10381 ccgcgcacgc agggaaaaac atggcgcaat ccttcaccgg caagaagcgg atccggaagt
    10441 ctttcggccg catccccgaa gctgtgcaga tgccgaacct catcgaggtt cagcggtcct
    10501 cctacgaaca gttcctgcag cgcgaagtgc gcagcggcga acgtcgcgac gagggcatcg
    10561 aggcggtctt caagtccgtc ttcccgatca aggacttcaa cgagcgcgcg gtgctcgaat
    10621 atgtctccta cgagttcgag gagccgaagt acgacgtcga ggaatgcatc cagcgcg
//
LOCUS       DAORNX010000044        10617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4652_length_10617_cov_8.007574, whole genome shotgun sequence.
ACCESSION   DAORNX010000044 DAORNX010000000
VERSION     DAORNX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4652_length_10617_cov_8.007574"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLO65_03285"
     CDS             complement(<1..320)
                     /locus_tag="PLO65_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HRD27304.1"
                     /translation="MTASPAKTMAETALEYGLKPEEYDLIVRRLNREPNAVELGVFSV
                     MWSEHCSYKSSRKHLGKFPTTGPRVICGPGENAGVIDIGDGQACVFKMESHNHPSYIE
                     PYQGA"
     gene            379..987
                     /locus_tag="PLO65_03290"
     CDS             379..987
                     /locus_tag="PLO65_03290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD27305.1"
                     /translation="MKQDRRTVAAALAGLPLLTGVAWARTPPTRVRIETAPGAIVVEL
                     YGDKAPVTVANFLRYVDEARFDGAEFYRTVRPEAMPQAGLLQGGCHGDPKRLLPPIAH
                     ESTDQTGLRHKVGTLSMPRRELGTADGEFFICLEDAPWYDVNPIKPGDNLGYAAFGQV
                     VEGLDVVRTIHAMPTSPTEGADYGMAGQILDPPVIMTAVRRV"
     gene            1121..1306
                     /locus_tag="PLO65_03295"
     CDS             1121..1306
                     /locus_tag="PLO65_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27306.1"
                     /translation="MTGNDRNPQQQQNDPQRQNPGQQQQQGGTPERNREQGGESGQRD
                     REQADRMDREGGERADR"
     gene            complement(1370..1900)
                     /locus_tag="PLO65_03300"
     CDS             complement(1370..1900)
                     /locus_tag="PLO65_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27307.1"
                     /translation="MDISQINWAYEALAVSAVLLVIWVLVARRARAGAPAAFVGLAHL
                     LVAGLHSAAPVRGYLDPDYVGYGFGLLAAERGLTVAVTAGTVWIVAVLAAFLALSRAR
                     AAMAFVALSSLAFAVIIGAPLVRGLLADPDASRIQLGEYLTIPGLAAVALLLTLLVLP
                     FLVGLVWAGRRALRRA"
     gene            complement(2085..2747)
                     /gene="purQ"
                     /locus_tag="PLO65_03305"
     CDS             complement(2085..2747)
                     /gene="purQ"
                     /locus_tag="PLO65_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRD27308.1"
                     /translation="MKAAVIVFPGSNCDRDCKVAIERSTGASVDMVWHGDSDLPDGLD
                     LIVLPGGFSYGDYLRCGAMAAQSPVMQAVRAAAQRGVATVGICNGFQILCEAGMLPGA
                     LLRNEGLKYVCKPVALEVTNAQTRFTAAYRDRREVTMTIGNGEGNFFADEATLDRLEG
                     QGQVVFRYIDNPNGSARDIAGIVNEAGNVLGMMPHPDRAFEIDLGSADGALLFQSALA
                     SA"
     gene            complement(2748..2987)
                     /gene="purS"
                     /locus_tag="PLO65_03310"
     CDS             complement(2748..2987)
                     /gene="purS"
                     /locus_tag="PLO65_03310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRD27309.1"
                     /translation="MKATVHVFLKPGVLDVQGKAVESALHGLGWADVKDVRVGRVITF
                     DLPGDDRAAGEAEVKAMCDRLLANTVIESYRVEIA"
     gene            complement(3210..3968)
                     /locus_tag="PLO65_03315"
     CDS             complement(3210..3968)
                     /locus_tag="PLO65_03315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRD27310.1"
                     /translation="MTTRRKKIYEGKAKILYEGPEPGTLIQYFKDDATAFNAQKKAVL
                     EGKGVINNRISEFVMLKLGAIGIPTHFIKRLNLREQLIKEVEIVPLEVICRNIAAGGL
                     ATRLGLEEGTPLPRSIIEFCLKDDKLGDPMVAEEHITAFNWASTQEIDDIMAMTLRIN
                     DFLCGMFAAVGITLVDFKIEFGRIYDGEFARVILADEISPDSCRLWDSTTNEKLDKDR
                     FRRDMGEVIESYTEVARRLGIMKEMPTVIRGGLH"
     gene            4198..4515
                     /locus_tag="PLO65_03320"
     CDS             4198..4515
                     /locus_tag="PLO65_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HRD27311.1"
                     /translation="MSTFDDREKGFEKKFAHDAEQDFKAAARRNRMLAEWAAGLMGLE
                     SVEEYVRAVVKADFEQPGDEDVLRKVFGDLKGSGVTVSEGEVRMKMDELLAQAREQIK
                     AGG"
     gene            complement(4512..5003)
                     /locus_tag="PLO65_03325"
                     /pseudo
     CDS             complement(4512..5003)
                     /locus_tag="PLO65_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
     gene            complement(5002..6318)
                     /gene="purB"
                     /locus_tag="PLO65_03330"
     CDS             complement(5002..6318)
                     /gene="purB"
                     /locus_tag="PLO65_03330"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRD27312.1"
                     /translation="MIPRYARPEAVAVWSPETRYKIWFEIEAHAADAMAELGVIPKAA
                     AEAIWAGGKDAVWDPDRIDEIERTTKHDVIAFLTHVSEVVGPEARFLHQGMTSSDVLD
                     TCLSVQMSRATDLLLDDVDLVLAALKRRAFEHRLTPCVGRSHGIHAEPTTFGLKLAGH
                     YAEFQRAKERLAMARFEIATCAISGAVGTFANVDPRVEQHVANKLGLAVEPVSTQVIP
                     RDRHAAWFAALAVVASSIERLAIEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPIL
                     TENLTGLARLVRSAVVPALENVALWHERDISHSSVERGIAPDASIHLDFALRRLAGVV
                     ERLVIYPENMLRNLDRLGGLVHSQRVMLALTHKDVSREEAYAAVQSNAMKVWRGEGRF
                     IDFLKADPFVSANMTDAELEDLFDNGYHFKHVDTIFARVFGEQANA"
     gene            complement(6350..7852)
                     /locus_tag="PLO65_03335"
     CDS             complement(6350..7852)
                     /locus_tag="PLO65_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27313.1"
                     /translation="MTDATAGGVERPVYSNGYKSLVLAMLLAAYTFNFIDRTIIATIG
                     QAIKVDLKLTDTQLGLLGGLYFALLYTTLGIPIARLAERKSRVNIVTWSLVIWSGFTA
                     LCGFAQNFVMLAILRFGVGIGEAGCSPPSHSLISDYFEPKKRASALSIYAFGIPLGTM
                     IGAVAGGWLTQAFSWRVAFVIVGLPGIILAILFKVLVKEPPRGHSDVVEHPLEPEDLT
                     APAAPEPKPFSLANEARELWSVARVLFGKWPVLHMVLGVTIASFGSYGSGAFVPAYFV
                     RQFGLDYATVGLITGLVGGFSAGIGTLVGGFVADRAARRSAVWYPLVPAIGLLVATPI
                     YIAAYLQNSWQTAALILLIPGIFHYTYLGPTFGVVQNSVPVTQRATATALLFFFLNLI
                     ALGGGPVFTGWISDLLGQFNFSHPGQTVMGMFHSFGGDVGAGNFITDCPGGKAPEGAP
                     EALATACHAAGSLGTRQAIIISICFYAWASLHYFLAAIGMVRHMKSTAGG"
     gene            complement(7936..9318)
                     /locus_tag="PLO65_03340"
     CDS             complement(7936..9318)
                     /locus_tag="PLO65_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27314.1"
                     /translation="MTDAAAPSAGPAPDPASPAVPLVSVAYRRYALGLLLVIYTLNFL
                     DRQVLNILMEDIKIEFGLRDWQLGALSGLAFAVFYTTLGIPIARMAETRHRGYIIAGA
                     VFVWSGFTALCSVAGSFWSLLLYRIGVGVGEAGCTPPAHSLITDYVPKANRASAIAFY
                     SIGTPLGTVVGLVLGGLVADAYGWRTAFLIAGAPGVLVAIIAALTLVEPRKKFKAAIA
                     ATALASGAAATASPTLKAALAILATKKTFWLIAFAASIKAFIGYGHAPFTVSFFLREH
                     GAEVARLAAAFGLESRGFLGVALSAMGGVAGILGAWLGGVIADRYAAKDLRGYVVVPA
                     IASVVTIPIYITAVSVPSAAWAIPILAVPALLGTLWYGPVYASAQSLVPPHARATAAA
                     VILFIINLIGLGLGPLGVGILSDLFGEGMGMGPAQGIRWALIVSTLFGLVAAGLFWTA
                     RKTIREEMVS"
     gene            complement(9365..>10617)
                     /locus_tag="PLO65_03345"
     CDS             complement(9365..>10617)
                     /locus_tag="PLO65_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27315.1"
                     /translation="LLVIYTLNFLDRQVVNILAEPIKKDLGLHDWQLGMMTGLAFALF
                     YTVLGLPIARLAERANRAWIISGAIAAWSGFTLLCGFAQNFPQLILARIGVGVGEAGC
                     TPPAHSLITDYVPRDRRASAIAFYSIGTPLGSLLGMAMGGVVADAWGWRAAFLVAGAP
                     GLLMAALALLTLKEPRKAAIKGAPHAGPGVGEALQEIAGKKTFWLMAIGAAIIAFAGY
                     GSAAFIGSFYFRNHAAEAAQAAAGFGLGVAGFLGLALGIIGGVAGILGSFTGGVIGDR
                     LGKQGPQGQMTLPAVAAIVGAPFYIFGMTTPSMIAAMAVLAIPTFLNSMWYGPVYGSV
                     QGLVRPQTRATAAAVILFIINLIGLGFGPLAVGALSDFYADGLGMGEGEGIRWALMTS
                     ASTMLVAAALFWTARKTIRTDMVS"
BASE COUNT         1783 a         3530 c         3768 g         1536 t
ORIGIN      
        1 gcgccctggt agggctcgat gtaggacggg tggttgtggc tctccatctt gaagacgcag
       61 gcctggccgt cgccgatgtc gatcacgccg gcattctcgc ccggtccgca gatcacccgc
      121 gggccagtgg tcgggaactt gccgaggtgc ttgcgtgagg acttgtacga gcagtgctcg
      181 gaccacatca ccgagaatac gcccagttcc acggcgttgg gctcgcggtt cagccggcgg
      241 acgatgaggt cgtattcctc gggcttgagg ccgtactcga gggccgtctc ggccatggtc
      301 ttcgcggggg aggcagtcat ggcgcgggct tagcggattc cgctcgaaat gtcagggcgg
      361 gcgaggcacg atggcggcat gaaacaggat cgcagaacgg tcgccgccgc cctggccggc
      421 ctaccccttc tgacgggtgt cgcctgggcc cggacgccgc cgacgcgggt gaggatcgag
      481 acggcgccag gagccatcgt tgtcgagctt tatggcgaca aggcccccgt cacggtggcc
      541 aacttcctgc gctacgtcga cgaggcgcgc ttcgacggcg cggagttcta tcgcacggtc
      601 cgtcccgagg cgatgccgca ggccgggctg ctgcaaggcg gctgccacgg cgacccgaaa
      661 cggctgttgc ctccgatcgc ccatgaatcg acagaccaga cggggcttcg ccacaaggtt
      721 gggaccctgt ccatgccccg gcgcgaactc ggcaccgccg acggcgagtt cttcatctgc
      781 ctcgaggacg cgccctggta tgatgtgaat ccgatcaagc cgggggacaa cctgggctat
      841 gccgccttcg gccaggtggt cgagggactg gacgtggtcc ggacgatcca cgccatgccg
      901 acctcgccga ccgaaggcgc ggactacggc atggccggcc agatcctcga cccgccggtg
      961 atcatgaccg ccgtccggcg ggtctgaccc ctgagtcgac cggtcgacac gggtctctct
     1021 cagccgtgat cgagcgggcc ggaacggccc cgagtcgcca cccgttctat ccctgcgccg
     1081 ccagtttccg gcggggcgcg taacccaggg agagacccgc atgaccggca acgaccgcaa
     1141 tccgcaacag cagcagaacg atccccagcg ccagaacccc ggccagcagc aacagcaggg
     1201 cgggaccccc gaacgcaacc gcgaacaggg cggggagtcc ggccagcgcg atcgcgagca
     1261 ggccgaccgc atggaccgcg agggcggaga gcgcgccgac cgctagcggc ggcggcgccc
     1321 aagggcggag caaagggccg gcggcagacg ccccggccct ttcgttcgtc taggcccggc
     1381 gcagcgcgcg gcgtccggcc cagaccagac cgacaaggaa gggcagcacc agcagggtca
     1441 gcagcagggc caccgccgcc agcccgggga tggtcaggta ttcgcccagc tggatcctgc
     1501 tggcgtccgg gtcggccagc agtccgcgga ccagcggcgc gccgatgatc accgcgaagg
     1561 ccaggctcga cagcgccacg aaggccatcg ccgcccgcgc tcgcgacagg gccaggaagg
     1621 ccgccaggac cgcgacgatc cacaccgtcc cggccgttac ggcgacggtc aggccgcgct
     1681 ccgcggccag caggccaaag ccgtagccga cgtagtcggg gtcgaggtag ccgcgcaccg
     1741 gagcggccga atgcaggccg gccaccagca gatgggccag gccgacgaag gccgccggtg
     1801 cgccggcgcg ggcccgcctg gcgaccagga cccagatcac gagcaggacg gccgatacgg
     1861 ccagcgcctc ataggcccag ttgatctgac tgatgtccat gcccgcctcc ggtgaagagc
     1921 cggaaggctg gccgaggccc agggccttga ccagtgaatt ccccgacagg ccgaaccggc
     1981 ggggtcgatc ggtccttcga cacggccgcg ccgcggcccg cccggcagac gtgaatggat
     2041 cccgcgtcag ggcgccgtgt gcgggtcgaa ccgcgcacgc cggatcaggc gctggccagc
     2101 gcgctctgga acagcagggc gccgtcggcg gagcccaggt cgatctcgaa ggcccggtcg
     2161 gggtggggca tcatgcccag cacgttgccg gcctcgttga cgatgccggc gatgtcgcgc
     2221 gccgagccgt ttggattgtc gatgtagcgg aacaccacct ggccctggcc ctcgaggcgg
     2281 tcgagcgtgg cctcgtcggc gaagaagttg ccctcgccgt tgccgatggt catcgtcacc
     2341 tcgcgccggt cgcgataggc ggcggtgaat cgcgtctggg cgttggtcac ctccagggcc
     2401 accggcttgc agacgtattt caggccctcg ttgcgcagca gggcgcccgg cagcatgccg
     2461 gcctcgcaga ggatctggaa gccgttgcag atgcccaccg tggccacgcc gcgctgggcc
     2521 gcggccctga ccgcctgcat caccggcgac tgcgccgcca tggccccgca acgcaggtag
     2581 tcgccatagg aaaagccgcc cggcagcacg atcaggtcca ggccgtccgg caggtcgctg
     2641 tcgccgtgcc agaccatgtc gacgctggcc ccggtcgagc gttcgatggc gaccttgcag
     2701 tcccggtcgc agttggaacc ggggaagacg atgacggcgg ctttcaccta ggcgatctcc
     2761 acccggtagc tttcgatcac ggtgttggcc agcagcctgt cgcacatggc cttgacctcg
     2821 gcctcgccgg cggcgcggtc gtcgccgggc agatcgaagg tgatcacccg gccgacccgg
     2881 acgtccttca catcggccca gcccagacca tgcagggcgc tctcgacggc cttgccctgc
     2941 acgtcgagga cgccgggctt gaggaagacg tggacggtgg ctttcattcg acttcggcct
     3001 cggcgctcgg gtcccccgcc aacagggggc atgctggatc gattccttct ccccttgcgg
     3061 gagaaggtgg cctccgaagg aggccggatg aggggtcgaa ggcggcgtcc gcctcgatca
     3121 ctcgcttggc cttgccgacg gaccggagaa acccctcatc cgtcaaggct ccgcctgaca
     3181 ccctctcccg caaggggaga agggcgcctt cagtgcagac cgccgcggat gaccgtcggc
     3241 atttccttca tgatgcccag tcgccgggcg acctcggtgt agctctcgat gacctcgccc
     3301 atgtcgcggc ggaagcggtc cttgtcgagc ttctcgttgg tcgtggaatc ccacagccgg
     3361 cagctgtcgg ggctgatctc gtcggccagg atcacccgcg cgaattcgcc gtcgtagatg
     3421 cgcccgaact cgatcttgaa gtcgaccagg gtgatgccga cggccgcgaa catcccgcag
     3481 aggaagtcgt tgatccgcag ggtcatggcc atgatgtcgt cgatctcctg ggtcgaggcc
     3541 cagttgaacg cggtgatgtg ctcctcggcg accatcgggt cgcccagctt gtcatccttg
     3601 aggcagaact cgatgatcga gcggggcagg ggcgtgccct cttccaggcc cagacgcgtg
     3661 gccaggccgc cggcggcgat gttgcggcag atcacctcca gagggacgat ctcgacttcc
     3721 ttgatcagct gttcgcgcag attcagccgc ttgatgaagt gggtcgggat gccgatggcg
     3781 cccagcttca gcatgacgaa ttcgctgatc cggttgttga tcacgccctt gccttcgaga
     3841 acggccttct tctgggcgtt gaacgccgtg gcgtcgtcct tgaagtactg gatcagggtg
     3901 ccgggctcag gcccctcgta gaggatcttg gccttgccct cgtagatttt cttgcggcgg
     3961 gtcgtcatgt cggtgttccc cgggggggtg cgatgcggcc tggctgaaca aaaatcgcgc
     4021 gcgggcggga agccggcgcg cggggttcga ctcaggggcc gctgtcgctg aagtcgagcc
     4081 ggaaactacc cgaacggggc ctccggcgca aacgccgcgc gggcgaggcg atccacctca
     4141 ttgcggcggc gcacacaagg ggatatcagg gcgcccggaa agaccgctgg agttttcatg
     4201 agcacgttcg acgatcgcga aaagggcttc gagaagaagt tcgcgcatga cgccgagcag
     4261 gacttcaagg ccgccgcgcg ccgcaaccgc atgctcgcgg aatgggccgc cggcctgatg
     4321 ggcctggaat cggtggaaga gtacgtgcgc gccgtggtga aggccgactt tgaacaaccc
     4381 ggcgacgagg atgtcctgcg caaggtgttc ggcgacctca agggctctgg cgtgaccgtc
     4441 agcgagggcg aggtccgcat gaagatggac gaactgctcg cccaggcgcg cgaacagatc
     4501 aaggccggcg gctagtccgc gagggcgtcg cgcaggccgt tccagacggc cgctttttgc
     4561 atcacgccca ggctcgcatg ggccgggctc gaggagggca gcgtcaccag acgcagccgg
     4621 tcctggtgcg tggccagcag gcggtggccg tcacgcgcgg ccttgccgcc attgaacgcc
     4681 accacccgca gatcaggcag cgacccgacc agggccgcaa ggtcgttgtg ggtggcgtcg
     4741 cggatcgcgg tgtccaggct gcccgcccgg gtcgcctcgc cgacgacatc ccacagtccc
     4801 acgcgatggc gcagcagggc cgccagccgg tcgtcgtagg gcagggcggc gatatcctcg
     4861 ccgatcaccc cgccgatcag ccgccagaag gcgttctgcg gatgggcgta gtagcgcccc
     4921 gcggccagcg acgcctcgcc gggcaggctg cccagcacca gcagccgcgt gtcggccgcg
     4981 acaaccggcg ggaagctgcg cttacgcgtt ggcctgttcc ccgaacaccc gggcgaagat
     5041 cgtgtccacg tgcttgaagt ggtagccgtt gtcgaacagg tcctcgagct cggcgtcggt
     5101 catgttggcg ctgacgaacg ggtcggcctt gaggaagtcg atgaaccggc cctcgccgcg
     5161 ccacaccttc atcgcgttgc tctggaccgc cgcataggcc tcctcgcggc tgacgtcctt
     5221 gtgggtcagg gccagcatga cgcgttggct gtgcaccagg ccgcccagcc ggtcgaggtt
     5281 cctgagcatg ttctccggat agatcaccag ccgctcgacc accccggcca ggcggcgcag
     5341 ggcgaagtcg aggtggatcg aggcgtccgg ggcgatgccg cgctcgaccg acgaatggct
     5401 gatgtcgcgc tcgtgccaga gggcgacgtt ctccaatgcc gggacaacgg ctgaccggac
     5461 caggcgggcg aggccggtca ggttctcggt caggatcgga ttgcgcttgt gcggcatcgc
     5521 cgaactgccc ttctggccgg gatcgaacgg ctcctcggcc tccagcacct cggtgcgctg
     5581 caggtggcgg atctcgatgg ccagccgttc gatggacgag gcgaccaccg ccagggcggc
     5641 gaaccaggcc gcgtgacggt cacggggaat gacctgggtc gagaccggct cgaccgccag
     5701 gcccaacttg ttcgccacat gctgttcgac acgcgggtcg acgttggcga acgtccccac
     5761 ggcgccggag atggcgcagg tggcgatctc gaacctcgcc atcgccagac gttccttggc
     5821 ccgctggaac tcggcgtagt ggccggccag cttcaggccg aaggtggtcg gctcggcatg
     5881 gatgccgtgg ctgcggccga cacagggcgt caggcgatgt tcgaatgcgc ggcgcttcag
     5941 ggcggccagc accaggtcga cgtcgtccag cagcaggtcg gtggcgcgcg acatctgcac
     6001 cgacaggcag gtgtcgagca catcgctgct ggtcatgccc tggtgcagga agcgggcttc
     6061 gggtccgacc acctccgaga cgtgggtcag gaaggcgatc acgtcgtgct tggtcgtgcg
     6121 ctcgatctcg tcgatgcggt cggggtccca gacggcgtcc ttgccgccgg cccagatggc
     6181 ctcggccgcc gccttgggga tgacgcccag ctcggccatg gcgtcggcgg cgtgggcctc
     6241 gatctcgaac cagatcttgt accgggtctc gggggaccag acggcgacgg cttcagggcg
     6301 ggcgtagcga gggatcatgc cgcccgctac gccctcaccg ggccaaccgt caaccgcccg
     6361 cggtcgactt catgtgcctg accatgccga tggccgccag gaagtagtgc agcgaggccc
     6421 aggcgtagaa gcagatcgag atgatgatcg cctgccgcgt gcccagggac ccggccgcgt
     6481 ggcaggccgt ggcgagggct tcgggcgcgc cctccggcgc cttgccgccc gggcaatcgg
     6541 tgatgaagtt cccggcgccg acgtcgccgc cgaagctgtg gaacatgccc atgaccgtct
     6601 ggccggggtg gctgaagttg aactggccga gcaggtcgct gatccagccg gtgaacaccg
     6661 gcccgccgcc cagggcgatc aggttgagga agaagaacag caaggccgtg gcggtcgccc
     6721 gctgggtcac cggcaccgag ttctgcacca ccccgaaggt cggacccagg taggtgtagt
     6781 ggaagatgcc ggggatcagc aggatcagcg ccgccgtctg ccagctgttc tgcagatagg
     6841 cggcgatgta gatcggcgtg gcgaccagca ggccgatggc cgggaccagc ggataccaga
     6901 cggcgctccg gcgcgccgcg cggtcggcga cgaagccgcc caccagggtg ccgatgccgg
     6961 ccgagaagcc gcccaccagg ccggtgatca ggccgacggt ggcgtagtcc aggccgaact
     7021 gacgcacgaa ataggccggc acgaaggcgc ccgagccata ggagccgaac gaggcgatgg
     7081 tcacgcccag aaccatgtgc agcacgggcc atttgccgaa caacaccctg gccacgctcc
     7141 acagttcgcg ggcttcgttg gccagtgaaa agggcttggg ttccggcgcg gccggcgcgg
     7201 tcaggtcctc cggctccagc ggatgttcga ccacatcgga gtgaccgcgc ggcggctcct
     7261 tgaccagcac cttgaacagg atcgccagga tgatgcccgg caggccgacg atgacgaagg
     7321 ccacccgcca gctgaacgcc tgggttagcc agccgccggc caccgcgccg atcatcgtgc
     7381 cgagcggaat gccgaaggcg tagatcgaca gcgccgacgc gcgcttcttg ggctcgaaat
     7441 agtcgctgat cagcgaatgc gagggcggcg agcagccggc ctcgccgatg cccacgccga
     7501 accggaggat ggccagcatg acgaagttct gggcgaagcc gcaaagcgcg gtgaatcccg
     7561 accagatgac cagcgaccag gtgacgatgt tcacccggct cttgcgctcg gccagccggg
     7621 cgatggggat gcccagggtg gtgtagagca gggcgaaata caggccgccc agcaggccca
     7681 gctgggtgtc ggtcagcttc aggtcaacct tgatcgcctg accgatggtg gcgatgatcg
     7741 tgcggtcgat gaagttgaac gtataggcgg ccagcagcat cgccagcacc agcgacttgt
     7801 agccgttcga atacacgggc cgctcgacgc cccccgccgt tgcatcggtc atgcaggcct
     7861 tcctcccttg tcgttaacgg cactctagcc gcgcccggcg gatcgccgcc agtcgcgaat
     7921 tgaagccccg gtatgtcagc tgaccatctc ttcgcggatc gtcttgcggg ccgtccagaa
     7981 caggccggcg gcgaccaggc cgaacagggt cgagacgatc agggcccaac ggatgccctg
     8041 ggccggtccc atgcccatgc cctcgccgaa caggtcgctg agaatgccga cgccgagcgg
     8101 gcccaggccc aggccgatca gattgatgat gaacaggatc accgccgcgg cggtggcccg
     8161 cgcgtgcggc ggcaccaggc tctgggccga ggcgtagacg ggtccgtacc acagggtgcc
     8221 cagcagcgcc ggcacggcga ggatcggaat ggcccaggcg gccgagggga cggacacggc
     8281 ggtgatgtag atcggaatgg tcaccaccga ggcgatggcc ggcaccacga cgtagccgcg
     8341 caggtctttg gcggcgtagc ggtcggcgat cacgccgccc agccaggccc cgaggatgcc
     8401 ggccaccccg cccatggcgc tcaacgcaac ccccaggaag ccgcgcgatt ccagcccgaa
     8461 ggcggcggcc agccgggcca cctcggcgcc atgctcccgc aggaagaagg acaccgtgaa
     8521 cggcgcatgg ccatagccga tgaaggcctt gatcgaggcg gcgaaggcga tcagccagaa
     8581 ggtcttcttg gtggcgagga tggccagggc ggctttcagg gtcggactgg ccgtggcggc
     8641 cgcgccgctg gccagggccg tggcggcgat cgccgccttg aacttcttgc ggggctcgac
     8701 cagcgtcagg gcggcgatga tggcgacgag cacgcctggc gcgccggcga tcaggaaggc
     8761 cgtccgccag ccgtaggcgt cggcgaccag gccgcccagg accaggccca cgaccgtccc
     8821 cagcggggtg ccgatggaat agaaggcgat cgccgaggcg cggttggcct tgggcacata
     8881 gtcggtgatc agcgaatggg cgggcggcgt gcagccggcc tcaccgaccc cgacgccgat
     8941 ccggtagagc agcaacgacc agaagctgcc ggcgacgctg cacagggcgg tgaatccgct
     9001 ccagacaaag acggcgccgg cgatgatgta tccccggtgc cgggtctcgg ccatgcgggc
     9061 gatcgggatg ccgagggtgg tgtagaacac cgcgaaggcg aggcccgaca gcgcgcccag
     9121 ctgccagtcg cgaagtccga actcgatctt gatgtcttcc atcaggatgt tgagcacctg
     9181 acggtcgagg aagtttagcg tgtagatgac cagcagcagg cccagggcat agcgccggta
     9241 ggcgacgctg acgaggggaa cggcggggct cgcggggtcg ggcgcggggc cagcggacgg
     9301 cgcggcggcg tcggtcattc cggggtttcc ttccgtgctt cggatctggg gcgttgaacc
     9361 tggcctagct gaccatgtcg gtcctgatgg tcttgcgggc ggtccagaac agggccgcgg
     9421 cgaccagcat ggtcgaggcc gaggtcatca gtgcccagcg gatgccttcg ccttcgccca
     9481 tgccgaggcc atcggcgtag aagtcgctca gggcgccgac cgccaggggg ccgaagccca
     9541 ggccgatcag gttgatgatg aacaggatca cggcggcggc ggtggcgcgg gtctgggggc
     9601 gcaccagacc ctgcacgctg ccatagaccg gcccgtacca catcgaattg aggaaggtcg
     9661 ggattgccag cacggccatg gccgcgatca tcgacggggt ggtcatgccg aagatataga
     9721 agggcgcgcc gacgatggcg gccacggccg gcagggtcat ctggccctgc ggaccctgct
     9781 ttccgaggcg gtcgccgatg acaccgcccg tgaacgagcc gagaatgccg gccacgcccc
     9841 cgatgattcc cagggccaga cccaggaagc cggccacgcc caggccgaac ccggcggcgg
     9901 cctgcgccgc ttcggccgcg tggttgcgga aatagaatga gccgatgaag gcggcgctac
     9961 cgtagccggc gaaggcgatg atcgcggcgc cgatggccat cagccagaag gtcttcttgc
    10021 cggcgatttc ctgcagcgct tcgccgacgc ccggaccggc gtgcggcgcg cccttgatcg
    10081 ccgccttgcg gggttccttc agggtgagca gggccagggc ggccatcagc aggcccgggg
    10141 ccccggcgac aaggaaggcc gcgcgccagc cccaggcgtc ggccaccacc ccgcccatgg
    10201 ccatgcccag cagcgagccg agcggggtgc cgatggaata gaaggcgatg gccgaggcgc
    10261 gcctgtccct cggcacatag tcggtgatca gcgaatgggc cggcggggtg cagccggcct
    10321 cgccgacccc cacgccgatc ctcgccagga tcagctgcgg gaagttctgc gcgaacccgc
    10381 acaacagggt gaagccactc caggcggcga tggcgccgga gatgatccag gcccggttgg
    10441 cgcgttcggc cagccgcgcg atcggcaagc ccagcaccgt gtagaacagg gcgaaggcca
    10501 ggccggtcat catgcccagc tgccagtcgt gcaggcccag gtccttcttg atcggctcgg
    10561 ccaggatgtt gaccacctgc cggtcgagga agttcagcgt gtagatgacc agcagca
//
LOCUS       DAORNX010000045        10483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4727_length_10483_cov_7.700710, whole genome shotgun sequence.
ACCESSION   DAORNX010000045 DAORNX010000000
VERSION     DAORNX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_10483_cov_7.700710"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1321
                     /locus_tag="PLO65_03350"
     CDS             182..1321
                     /locus_tag="PLO65_03350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27316.1"
                     /translation="MARDTADGFNEDRFAPVRAAFEANLASGADLGASFCATLEGETV
                     VDLWGGWADAEKTRPWARDTIVNVYSTTKTMTAMTALLLADRGELDFDAPVARYWPEF
                     AANGKADIKVSQLMSHSAGLSGWVEPVKGDILYDWERATALLAAQAPLWAPGTAPGYH
                     AVTQGYLVGEVVRRITGKSLGTVFREEFAEPLGADFHIGLPASEDGRVADLIPPPPND
                     ASIGEGASLYRTDNPPIDVLATRTRAWRGAEIPAAGGTGNARSVAEIHAILANGGVAK
                     GRRFLSEAGCRKALELQIEGKDLVLGMPVRYGMGFGLGGATLPLPSPNTIFWGGYGGS
                     LAIIDMDARTTFAYAMNRMAGTTTGDMRAFGLAMAMWQGLGLIPA"
     gene            complement(1395..3464)
                     /locus_tag="PLO65_03355"
     CDS             complement(1395..3464)
                     /locus_tag="PLO65_03355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD27317.1"
                     /translation="MKLISRLFAASVMALTIGSAVEARPFTPRDMVTLDRVSDPRVSP
                     DGRYVIYARRVADYEANKARTELWLVDLSGKTPAAPRMLDASAGGGVSSARWGADGHS
                     IYFLSSRGGSMQVWRADVNAAGASQVTKLPFDVGGFQTSRDGRKLVVSMAVFPDCPTL
                     QCSTDRMAARAAKKTTGVVYDRLFVRHWDEWADGTRNQLFVLAFDEKGITADGPVAVM
                     HGFDGDAPSKPFGDETEFGFSPDGKEVVFSARLAGKTEPWSTNFDIYGVPADGSGQLT
                     NYTEANKAWDTGVVFSPDGKFAAARIMKRPGFEADRWQIVIHDETTGKDRELAPDWDR
                     SAQTLAWSPDGKTLYTIAEDVGQTRLFAIDVKSGKVAPLTGDGHVSAFDVGPKGIVYA
                     QDDLDSPAQLFFIGLKGGKRTQVTNANADKLANLEFGGYEQFSFPGWNGETVHGYLVR
                     PVGFDPSKKYPVAFLIHGGPQGSFGNLFHYRWNPQTYAGAGYAVVMIDFHGSTGYGQA
                     FTDAISQHWGDRPLEDLQKGWAYALKSYSFLDGDRACALGGSYGGYMVNWIAGNWSQP
                     WKCLVNHDGVFDSRSMAYSTEELWFSEWENGGEAWIATANIERFNPANHVQDWRVPTL
                     VIQGGRDYRIPLEQGLSTFTALQRRGIESKLLYFPDENHWVLKPQNSVQWHDEVLGWL
                     DAHTRPR"
     gene            complement(3586..4986)
                     /gene="trmFO"
                     /locus_tag="PLO65_03360"
     CDS             complement(3586..4986)
                     /gene="trmFO"
                     /locus_tag="PLO65_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRD27318.1"
                     /translation="MTMNPVHVVGGGLAGSEAAWQLAEAGVPAVLHEMRPVRGTDAHQ
                     TDGLAELVCSNSFRSDDWQYNAVGLLHAEMRRCGSIILSSGDKHQVPAGGALAVDRDG
                     FSAEVTARLEAHPLVTIVREEVAGLPPADWDSVIIATGPLTSPALAEAILALTGEDSL
                     SFFDAIAPIVHLDSIDMSKAWRQSRYDKEGPGGDAAAYINCPMDREQYDAFVDALLAG
                     PKSEFKDWENVPYFDGCLPIEVMTERGRETLRHGPMKPVGLTNAHNPTVKPWAIVQLR
                     QDNALGTLWNMVGFQTKLKHGAQTDIFRMIPGLENAQFARLGGLHRNTFLNSPRLLDE
                     KLRLKADTRLRFAGQVTGVEGYVESAALGLLTGRLAAAERLGRPIPLPPATTALGALA
                     MHITGGFLDGPKASFQPMNINYGLLPPMEAPRQDAEGRKLHPQDRGRAKKRLMSERAL
                     ADLERWLAGEALAAAG"
     gene            5093..6022
                     /locus_tag="PLO65_03365"
     CDS             5093..6022
                     /locus_tag="PLO65_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27319.1"
                     /translation="MIKLDVVKAAFSGFGVIGRNPLAVLVWALFLGVVGVLPALALMG
                     GVFSAIGQLIAADRTGAEPTPEMIMPMVGSIIAIVPVLMLTGLIVRMMLTGAVFRAVL
                     FPEDRGWFYLRLGARELWLALLFVVVGIISFIVTMLLMGLVLPVVFLAGGHDDPLRTM
                     ILVRLMMLPVYGVLAFLFVRFCLAFPMTFAESKFRLMESWTMTRGNGWRILLVLLILI
                     ALMILVELVLVFLIAIAAVSIIGAGAMKGAWSEESLTAFFAQDPSVWMQTLLPWVVGA
                     GAIGSILGVLVMVIFTAPWAEAYRQLRSSAPAA"
     gene            6134..6937
                     /locus_tag="PLO65_03370"
     CDS             6134..6937
                     /locus_tag="PLO65_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27320.1"
                     /translation="MSQARFSVRTAVAEGFEFWRGNVLRAIGPLVIAAGGLVLLLTAR
                     SFAALAAGFALYGLAGLAAQAALYRISLADAGAGPATRNGPFGLQWRGLETRFVAVSL
                     LTAALLGIVAVIAAFIIAGLLVGLIGVPAATTATTPEALLALLTPTGALIFNLAALAA
                     MAGLLVLNARLSLAFPATAAENRVRVISSIALTRGSVLRILGATLLINLPIILMQSLA
                     GLFGDAMRSPESSLWTQLIVGVISTFFYVPISVGMTSYIYRRLRGSAGQ"
     gene            6934..7806
                     /locus_tag="PLO65_03375"
     CDS             6934..7806
                     /locus_tag="PLO65_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27321.1"
                     /translation="MIISPSDTAFEGFRIIRERPGLILAWSGFYLLCLLAMILILLLP
                     NLGTLAAVEATGTPRDLSQLLARFGPPILIIFPMAMAMLIMLVTAVYRTVLTPEDKGF
                     AHLKIGADELRMFLVSLLVILIFAVASGLYGAAVVTAANASGRLHGAVTFIGSIGGVI
                     LLGWLAVRLALAGVMTFAEHHFRLGAAWRLTVGQFWRLLMTLVLTIFYAVLVVVLALT
                     FSLVLAQVTGGFSLMGELASPDLSNLTPAFALAMLGQLLLQLTLQTLLIVLVFVIFYA
                     PPAAAYRRLKSPPV"
     gene            complement(7803..8417)
                     /locus_tag="PLO65_03380"
     CDS             complement(7803..8417)
                     /locus_tag="PLO65_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27322.1"
                     /translation="MILYGENNPAPNPRRVRMFVAEKGIDLPETRINLRRREHKSDEH
                     RARNSLGQVPTLVLDDGTAISESVSICRYLESLHPSPALFGDTALDQARVDMWIRRVE
                     FQLMTPVGMFWRHAHPLTASLLTQFRDFGESNRETYGRVCQWLDADLADGRAFLAGEA
                     YSMADICAQTTVDFAAFTGLALPSETPHLTAWHDRVSARPSASA"
     gene            complement(8427..8732)
                     /locus_tag="PLO65_03385"
     CDS             complement(8427..8732)
                     /locus_tag="PLO65_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27323.1"
                     /translation="MKVLLLTLAAAASVATAVIAAEDPTPSAVPAAVPAPVAAPTTKK
                     DPNERICKTSPVIGTRVPSRTCMTRAEWEERARTDRQDLEASQRGGLTTCSTNPCGG"
     gene            8846..>9853
                     /locus_tag="PLO65_03390"
     CDS             8846..>9853
                     /locus_tag="PLO65_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27324.1"
                     /translation="MCDDFAQPCGAGASLASMVEECSPDPRKRAPERLLDKGAGVIMF
                     SATEAAFSGFRAGREHARTLLVWIPLLGLASLLMSAAMVVFGGPGLEQLEQLASESQP
                     DPQAMLAAAKPLASFYVIFLAIAVAYYGIVAAAVNRMMLRPGDRRMAYLALGADELRQ
                     VAVILLLTLAYSLLYVAGLVAVIVLAAVASAVHVALGVVVGLAGGLGLAALLVVVAVR
                     WSLASAQTFDTGRINLFGSWALTRGRFWPMLGAYLLAFILAIIANAAVLLLLMLAMVV
                     FGGGFSALGPIMSPDMTSLQTYFTPGLLLYELIGAIPAPFLLLIMMCPAPDIYRSLVG
                     RA"
     assembly_gap    9854..9871
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9879..>10483)
                     /locus_tag="PLO65_03395"
     CDS             complement(9879..>10483)
                     /locus_tag="PLO65_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD27325.1"
                     /translation="FLPDVYVTCDVCKGKRYNRETLEILFKGKSIADILDMTVDEAAD
                     FFKVVPPVRDKMETLKRVGLGYIHVGQQATTLSGGEAQRVKLSKELSRKATGRTLYIL
                     DEPTTGLHFEDTKKLLEVLHELVDHGNTVVVIEHNLDVVKTADWIVDFGPEGGDGGGE
                     IVGVGTPEKVAGIAGSWTGRYLAEVLKRHRDRAGEKRRRA"
BASE COUNT         1427 a         3516 c         3656 g         1866 t
ORIGIN      
        1 gacccgaaac accgcttggg ttcccgcctt cgcgggaaca atcggagccc ttggcagctc
       61 ccgcgcgctg aatgccgatt ggccctagcg gccaagggcc gccccgccgg ttcccctgcg
      121 tgaactggcg cgatcggcgg ccttcgtcgt atgggtgctc aacgacacag ggaggcgacg
      181 gatggccagg gatacggcgg acggattcaa tgaggaccgg ttcgcgccgg tccgcgcggc
      241 cttcgaggcc aacctcgcca gcggggccga cctcggcgcc tcgttctgcg ccaccctcga
      301 gggcgagacg gtcgtcgacc tgtggggagg ctgggccgac gccgaaaaga cccgtccctg
      361 ggcgcgcgac accatcgtca acgtctattc gacgaccaag accatgacgg cgatgaccgc
      421 gctgctgttg gccgatcgtg gcgaactcga cttcgatgcg cccgtcgccc gctactggcc
      481 ggagttcgcg gccaacggca aggcggacat caaggtcagc cagctgatga gccattcggc
      541 cggactgtcg ggctgggtcg agccggtcaa gggcgacatc ctctacgact gggagcgggc
      601 cacggcccta ctggccgccc aggccccgtt gtgggccccg ggcacggccc ccggctatca
      661 tgccgtgacc cagggctatc tcgtgggtga agtcgtccgg cgcatcaccg gcaagtctct
      721 gggcacggtg ttccgcgagg agttcgccga gccgcttgga gcggacttcc acatcggcct
      781 gccggcgtcc gaggacgggc gcgtggccga cctcattccg ccgccgccca atgacgcctc
      841 gatcggcgag ggggccagcc tctaccggac cgacaatccg ccgatcgacg tgctggcgac
      901 ccgcacccgg gcctggcgcg gcgccgagat accggcggcc ggcggcacgg gcaacgcccg
      961 gtcggtggcc gagatccacg ccatcctggc caacggcggc gttgcgaaag ggcggcgctt
     1021 cctgtccgaa gccggctgtc gcaaggccct ggagctgcag atcgagggca aggacctcgt
     1081 gctcggcatg ccggtccgct acggcatggg cttcggactc ggcggcgcga ccctcccgct
     1141 gcccagcccc aacacgatct tctggggcgg ctatggcggc tcgctggcga tcatcgacat
     1201 ggatgcgcgc acgaccttcg cctacgccat gaaccgcatg gccggcacga ccaccggcga
     1261 catgcgcgcg ttcggcctgg ccatggccat gtggcagggt ctcggcctga tcccggcctg
     1321 actctcgatc acaataggca agggggacgc cgcgatgcga cgccccccaa tcttcgcagc
     1381 ctcggacccg gccgttaccg cggcctggtg tgggcgtcga gccagccgag cacctcgtcg
     1441 tgccactgca ccgagttctg cggcttgagc acccagtggt tttcgtccgg gaagtacagc
     1501 agcttgctct cgatcccacg ccgctggagg gcggtgaagg tgctcaggcc ctgctccagg
     1561 ggtatgcgat agtcgcggcc gccctggatc acgagggtcg ggacgcgcca gtcctggaca
     1621 tggttggccg ggttgaaccg ctcgatgttg gccgtcgcga tccaggcttc gccgccgttc
     1681 tcccactcgc tgaaccagag ctcctcggtg ctgtaggcca tcgaccggct gtcgaagacg
     1741 ccgtcgtggt tgaccaggca cttccacggc tggctccagt tgccggcgat ccagttgacc
     1801 atgtagccgc cgtacgagcc gcccagggcg caggcgcggt cgccgtccag gaagctgtag
     1861 gacttcaagg cgtaggccca gcccttctgc aggtcttcca gcgggcggtc gccccagtgc
     1921 tggctgatcg cgtcggtgaa ggcctggccg tagccggtcg agccatggaa atcgatcatc
     1981 accaccgcat agccggcccc ggcgtaggtc tgggggttcc agcggtagtg gaacaggttg
     2041 ccgaagctgc cctgcggccc gccatggatc aggaaggcga ccgggtactt cttcgacggg
     2101 tcgaaaccga ccggcctgac caggtagccg tgcacggtct cgccgttcca gccggggaag
     2161 ctgaactgct cgtagccgcc gaactccagg ttggccagct tgtcggcgtt ggcgttggtc
     2221 acctgcgtcc gcttgccgcc cttgaggccg atgaagaaca gctgcgccgg gctgtcgagg
     2281 tcgtcctggg catagacaat gcccttcggg ccgacgtcga aggcgctgac atggccgtcg
     2341 ccggtcaggg gcgcgacctt tccggacttg acgtcgatcg cgaacagccg ggtctggccg
     2401 acgtcctcgg cgatcgtgta gagcgtcttg ccgtccggcg accaggccag ggtctgggcc
     2461 gagcggtccc agtccggggc cagttcgcgg tccttgccgg tggtctcgtc gtggatgacg
     2521 atctgccagc ggtcggcctc gaagcccggg cgcttcatga tccgcgcggc ggcgaacttg
     2581 ccgtccggcg agaacaccac gccggtgtcc caggccttgt tggcctcagt atagttggtc
     2641 agctggcccg agccgtcggc cgggacgccg tagatgtcga aattggtgct ccagggctcg
     2701 gtcttgccgg ccagccgggc ggagaacacc acctccttgc catctggcga gaagccgaac
     2761 tcggtctcgt cgccgaacgg cttggacggc gcgtcgccgt cgaagccgtg catcacggcc
     2821 acggggccgt cggcggtgat gcccttctcg tcgaaggcca gcacgaacag ctggttgcgc
     2881 gtgccgtcgg cccattcgtc ccagtggcgc acgaacagcc ggtcgtagac cacgcccgtt
     2941 gtcttcttcg ccgcgcgggc cgccatccgg tcggtgctgc actgcagggt cgggcagtcg
     3001 gggaacacgg ccatcgaaac gaccagcttc cggccgtccc gcgaggtctg gaacccgccc
     3061 acgtcgaacg gcagcttcgt cacctggctg gcgccggccg cgttcacgtc ggcgcgccag
     3121 acctgcatcg acccgccacg cgaggacagg aaatagatgc tgtggccgtc ggcgccccag
     3181 cgggcgctgc tgacgccgcc gcccgccgag gcgtcgagca tgcgcggcgc ggccggagtc
     3241 ttgcccgaca ggtcgaccag ccaaagctcg gtcctggcct tgttggcctc gtagtcggcc
     3301 acccgccggg cgtagatcac gtagcggccg tcgggcgaga cccgcggatc gctgacgcgg
     3361 tccagggtga ccatgtccct gggcgtgaag ggccgcgcct cgacggcgga gccgatggtc
     3421 agcgccatga cgctggcggc gaaaaggcga gagataagct tcattcaggc gtttcctggc
     3481 ggatcgttgc gcgattttgc gcgcacccta agggcgtcgt gcgccaggcg ccagtggccg
     3541 gggcagcaat ttccggcctc tcgcggagcc cggtcgaccg cccatctacc cggccgcggc
     3601 gagagcctca ccggccagcc agcgctccag atcggccagg gcccgttcgc tcatcagccg
     3661 cttcttcgcc cggccgcgat cctgcgggtg cagcttgcgg ccctcggcgt cctggcgcgg
     3721 ggcttccatc ggcggcagca ggccgtagtt gatgttcatc ggctggaagc tggccttcgg
     3781 cccatcgagg aagccgccgg tgatgtgcat ggccagggcg ccgagcgcgg tggtggcggg
     3841 aggcagggga atcggccggc ccagccgctc ggccgcagcg aggcgtccgg tcagcaggcc
     3901 cagcgccgcg ctctccacat agccctccac gccggtcacc tggccggcga agcgcaggcg
     3961 ggtgtcggcc ttcagccgca gcttctcgtc cagcagccgc gggctgttga ggaaggtgtt
     4021 gcggtgcagc ccgcccaggc gcgcgaactg ggcgttctcc aggcccggga tcatgcggaa
     4081 gatgtcggtc tgggccccgt gcttcagctt ggtctggaag ccgaccatgt tccaaagggt
     4141 gcccagcgca ttgtcctgcc gcagctggac gatggcccag ggcttgaccg tcggattgtg
     4201 ggcgttggtc aggccgaccg gcttcatcgg cccgtgacgc agggtctcgc ggccacgctc
     4261 ggtcatcacc tcgatcggca ggcagccgtc gaaatagggc acgttctccc agtccttgaa
     4321 ctcggacttg ggcccggcca gcagggcgtc gacgaaggcg tcgtactgct cgcggtccat
     4381 cgggcagttg atgtaggcgg ccgcgtcgcc gcccggacct tccttgtcgt agcgcgactg
     4441 ccgccaggcc ttgctcatgt cgatcgagtc gagatgcacg atcggcgcga tggcgtcgaa
     4501 gaagctgagg ctgtcctcgc ccgtcagggc gaggatcgcc tcggccaggg cgggcgaggt
     4561 gagggggccg gtggcgatga tcacgctgtc ccagtcggcc ggcggcaggc cggcgacttc
     4621 ctcgcggacg atggtcacca gcgggtgggc ctcgagtcgg gcggtgacct cggcggagaa
     4681 gccatcgcgg tccacggcca gggcgccgcc ggccggcacc tggtgcttgt cgccgctgga
     4741 caggatgatc gagccgcagc ggcgcatctc ggcgtgcagc aggcccacgg cgttgtactg
     4801 ccagtcgtcg gagcggaacg agttggagca gaccagctcg gcgaggccgt cggtctggtg
     4861 ggcgtcggtg ccgcgcaccg ggcgcatttc gtgcaggacg gccggcacgc cggcttccgc
     4921 cagctgccag gccgcttcgg atccggccag gccgccgccg actacgtgaa caggattcat
     4981 ggtcatggcg gacagatagg ccgaccggcc ccgcgcgcaa agccggccaa ggtggcgccg
     5041 aggccgattt tatgatcgtc tcggccgtca acgaaacgcg gggaggcgtg acgtgatcaa
     5101 gctggatgtt gtgaaggcgg ccttttcagg gtttggcgtc atcgggcgaa atccgctggc
     5161 cgtgcttgtc tgggctctgt ttctgggtgt ggtgggcgtg ttgcccgccc tggcccttat
     5221 ggggggtgtg ttcagcgcca tcggtcagtt gatcgccgcg gatcggaccg gcgccgaacc
     5281 gaccccggaa atgatcatgc cgatggtcgg atcgatcatc gccatcgtgc cggtgctgat
     5341 gctgaccggc ctgatcgtca ggatgatgct gaccggcgcg gtgttccggg cggtgctgtt
     5401 tcccgaggac cggggctggt tctatctgcg cctcggggct cgcgagctct ggctggccct
     5461 gctgttcgtg gtcgtcggaa tcatcagctt catcgtcacc atgctcctga tgggcctggt
     5521 gttgccggtg gtgttcctcg ccggcggcca cgatgatccc ctgaggacga tgatcctcgt
     5581 ccgcctgatg atgctgccgg tctacggcgt gctggccttc ctgttcgtcc ggttctgcct
     5641 cgcctttccg atgaccttcg ccgagtcgaa gtttcggctg atggagtcct ggacgatgac
     5701 ccgcggcaac gggtggcgga ttctgctcgt gctgctgatc ctgatcgccc tgatgatcct
     5761 cgtcgagctg gtcctggtgt tcctgatcgc gatcgcggcg gtgtcgatta tcggcgccgg
     5821 ggcgatgaag ggcgcctgga gcgaggagag cctgaccgcc ttcttcgccc aggacccctc
     5881 ggtctggatg cagacgctgc tgccgtgggt tgtcggcgcg ggcgcgatcg gatcgatcct
     5941 cggggtgctg gtcatggtca tcttcaccgc cccctgggcc gaggcctacc ggcaattgcg
     6001 ctcctcggcc ccggccgcgt aggggcgcct gcggcgggcg cttcattgcg gaccgcaaat
     6061 ccctgtcgcc acagggcgcg aagcagggtt taagtcggcc gggaaccatg cgcgccacgg
     6121 cgcggggagt cgtatgtcgc aggcgcgttt ttcggtccgg acggcggtgg ccgaaggctt
     6181 cgagttctgg cgcggcaacg tgctcagggc gattggtccg ttggtgatcg cggccggcgg
     6241 cctggtgctg cttctgacgg ccaggtcctt cgcggctctg gccgcaggct tcgccctcta
     6301 tggcctcgcc ggcctggccg cccaggccgc cctctaccgc atcagcctgg ccgacgccgg
     6361 cgccgggccg gcgacccgca acggaccgtt cggcctgcaa tggcggggtc tggagacccg
     6421 tttcgtcgct gtcagcctgc tgacggcggc ccttctgggc atcgtggcgg tgattgcggc
     6481 cttcattatc gccggcctgc tggtcgggtt gatcggcgtc ccggcggcca ccacggcaac
     6541 cacgccggag gcgctgctgg ccttgctgac gccgacgggc gccctgatct tcaatctcgc
     6601 cgccctggcc gccatggccg gcctgctggt gctcaacgcg cggctgtcgc tggcctttcc
     6661 cgccacggcg gcggagaacc gggtccgcgt gatcagcagc atcgccctga cacgggggtc
     6721 ggtcctgcgc atccttggcg ccaccctgct gatcaacctg ccgatcatcc tcatgcagtc
     6781 gctcgccgga ctgttcggcg acgccatgcg cagtccggag tcgagtctct ggacccagct
     6841 gatcgtcggg gtgatctcga ccttcttcta cgtgcccatc tccgtgggca tgacctccta
     6901 catctatcgc cggctgcggg ggagcgcggg ccaatgatca tttcgccatc ggacacggcc
     6961 ttcgagggct tccggatcat tcgcgaacga cccggcctga tcctggcctg gtcgggcttc
     7021 tacctgctgt gcctgctggc gatgatcctg atcctgctgc tgcccaacct cggcaccctg
     7081 gccgcggtcg aggccaccgg caccccgcgc gacctgagcc agctgctggc gcggttcggt
     7141 ccgccgattc tgatcatctt ccccatggcc atggcgatgc tgatcatgct ggtcacggcg
     7201 gtgtaccgca cggtgctgac accggaggac aagggcttcg cccacctgaa gatcggcgcc
     7261 gacgaactgc gcatgtttct tgtcagcctg ctggtcatcc tgatcttcgc tgtggccagc
     7321 ggcctctacg gcgcggcggt ggtgacggcg gccaacgcgt cggggcggct gcacggggcg
     7381 gtgaccttta tcggctcgat cggcggggtg atcctgctgg ggtggctggc ggtgcggctt
     7441 gccctggccg gcgtgatgac cttcgcggaa caccacttcc ggctcggggc ggcctggcgg
     7501 ctgacggtcg gccagttctg gcggctgctg atgaccctgg tgctgacgat cttctacgcc
     7561 gtgctggtgg tggtgctggc gctgaccttc agcctcgtcc tggcccaggt gaccggcgga
     7621 ttttcgctga tgggcgaact ggccagtccg gacctgtcga acctcacccc ggcgttcgcc
     7681 ctggcgatgc tgggtcagct gctgctccag ctgacgctgc agacactgct gatcgtgctg
     7741 gtgttcgtga tcttctacgc cccgccggcg gcggcctacc gccggctcaa gtcgccgccg
     7801 gtctaggccg acgcgctcgg gcgggccgag acccggtcat gccatgccgt gaggtgcggc
     7861 gtctccgacg gcagcgccag cccggtgaag gcggcgaaat cgacggtggt ctgggcgcag
     7921 atgtcggcca tcgaataggc ctcgcccgcc aggaaggccc gaccatcggc cagatccgca
     7981 tcaagccact ggcagacccg cccataggtc tcgcggttgg attcgccgaa atcgcgaaac
     8041 tgcgtcagca gggacgcggt cagcgggtgg gcgtggcgcc agaacatgcc gaccggcgtc
     8101 atcagctgga attcgacccg gcgaatccac atgtcgaccc gcgcctggtc cagcgccgtg
     8161 tcgccgaaca gggccggcga agggtgcagg ctctcgagat agcggcagat cgagacgctc
     8221 tcggagatcg ccgtgccgtc gtcgagcacc agggtcggca cctggcccag cgagttccgg
     8281 gcccggtgtt cgtccgactt gtgctcacgc cgacggagat tgatccgtgt ctcggggagg
     8341 tcgatgccct tttcggcgac gaacatcctc acccggcgcg ggttcggcgc cgggttgttc
     8401 tcgccgtaga ggatcatgtc aggcgatcag ccgccgcagg ggttggtgct gcaggtcgtc
     8461 aggccgccgc gctgcgaggc ctcaaggtcc tgacggtccg tgcgggcccg ttcttcccac
     8521 tcagcgcggg tcatgcaggt gcggctcggc acccgcgtgc cgatcaccgg cgaggtcttg
     8581 cagatccgct cgttgggatc cttcttggtc gtcggggcgg cgaccggcgc ggggacggcg
     8641 gccggaacgg ccgacggcgt cgggtcctca gcggcgatca cggcggtggc gacggacgcg
     8701 gcggcggcaa gcgtaagcag aagaaccttc acagggaccc ccaggggaaa ttggaacgcg
     8761 gattgggatc tcgtcaccaa accagaagct gacagaagag cacagctcgc ctcgcctgtc
     8821 atcgtccgga gcgcgcaacg ggacgatgtg tgacgatttc gcccaaccct gcggcgccgg
     8881 cgcctcgctt gcctcgatgg tcgaggaatg ctcccctgat ccgcggaagc gggcgccgga
     8941 gcgcctgctg gacaagggag cgggcgtcat catgttttcg gccaccgaag cggctttcag
     9001 cgggttccga gctggtcgcg agcatgccag gaccctgctg gtgtggattc cgctgctggg
     9061 cctggcctcg ctgctgatgt cggcggcgat ggtcgtgttc ggcggtccgg ggcttgagca
     9121 gctcgaacag ctggccagcg aatcgcagcc cgatccgcag gccatgctgg cggcggcgaa
     9181 gccgctggcg tccttctacg tgatcttcct cgccatcgcc gtggcctatt acgggatcgt
     9241 cgccgcggcg gtgaatcgca tgatgttgcg gcccggagac cggcggatgg cctatctcgc
     9301 cctcggcgcg gacgagctgc gccaggtcgc cgtcatcctg ctgctgaccc tcgcctattc
     9361 actgctctat gtcgccggac tggtcgccgt gatcgtcctg gcggcggtcg cctcggccgt
     9421 tcacgtggcc ctcggcgtgg tggtcggcct ggccggcggc ctgggcctcg cggccctgct
     9481 ggtggtggtg gccgtgcgct ggtcattggc cagcgcccag accttcgaca cgggccggat
     9541 caacctgttc ggctcctggg ccctgacgcg cggccggttc tggccgatgc tgggcgccta
     9601 tctactggcc ttcatcctgg cgatcatcgc caacgccgcc gtcctgctgc tgctgatgct
     9661 ggcgatggtg gtgttcggcg gagggttctc cgccctcggg ccgatcatgt cgccggacat
     9721 gacttcgctg cagacctact tcaccccggg cctgctgctg tacgagctga tcggggcgat
     9781 ccccgcgccg ttcctgctgc tgatcatgat gtgccccgcg ccggacatct accgcagcct
     9841 ggtcggtcgc gcgnnnnnnn nnnnnnnnnn ncgatcggtc aggcccgtcg gcgcttttcc
     9901 ccggcgcggt cgcgatgtcg cttgagcacc tcggcgaggt agcgcccggt ccagctgccg
     9961 gcgatgccgg cgaccttctc gggcgtgccg acgccgacga tctcgccgcc gccgtcgccg
    10021 ccctcggggc cgaagtcgac gatccagtcg gcggtcttca ccacatcgag attatgctcg
    10081 atgaccacca cggtgttgcc gtggtcgacc agttcgtgga gcacctcgag cagcttcttg
    10141 gtgtcctcga aatgcagccc ggtggtcggc tcgtcgagga tgtagagggt ccgaccggtc
    10201 gccttgcggc tcagctcctt tgacagtttg acccgctggg cctcgccgcc ggacagggtg
    10261 gtcgcctgct ggccgacgtg gatgtagccc aggcccacgc gcttgagcgt ctccatcttg
    10321 tcgcgcaccg gcggcacgac cttgaagaag tcggccgcct catcgaccgt catgtcgaga
    10381 atgtcggcga tcgacttgcc cttgaacagg atttccagcg tctcgcggtt gtagcgcttg
    10441 cccttgcaga cgtcgcaggt gacgtagacg tcgggcagga agt
//
LOCUS       DAORNX010000046        10248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4851_length_10248_cov_7.794467, whole genome shotgun sequence.
ACCESSION   DAORNX010000046 DAORNX010000000
VERSION     DAORNX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_10248_cov_7.794467"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLO65_03400"
     CDS             complement(<1..503)
                     /locus_tag="PLO65_03400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRD27326.1"
                     /translation="MNNTLRTFILLAALTAIFVTAGFLIGGTGGMLIALVLSVGMNLF
                     SYWNADRIVLSMYRAVAVDETHPDPLVRNYVADVYALADGAGLPRPRAHVIESDQPNA
                     FATGRDPQHAAVAATSGLLRMLDRREIRGVMAHELAHVKNRDTLTMTVTATIAGAISG
                     LANIAFF"
     gene            complement(546..1085)
                     /locus_tag="PLO65_03405"
     CDS             complement(546..1085)
                     /locus_tag="PLO65_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRD27327.1"
                     /translation="MLRTILIFGLAAGLVVAVPMNLMMIFGKEGDGSHSLVTGYALML
                     LAFTFTFVGVKHHRDRALGGVIRFLPALLVGLGISVVASVIYVIAWEITLHATNFAFI
                     DDYSRAMLEGARAKGASAAELARMTAETETFRTQYASPLFRLPMTFIEIFPVGVVVSL
                     VTAGLLRNSRFLPMKVVRS"
     gene            complement(1185..1601)
                     /locus_tag="PLO65_03410"
     CDS             complement(1185..1601)
                     /locus_tag="PLO65_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD27328.1"
                     /translation="MWKTVLTYGLALALGAVALQWLDYRLLARTHTGEVWVALVAAGF
                     LGLGVWVGARLFRPRVLTGGFETNTPALAALGVTRREHEVLGLLAAGRSNKEIARQLN
                     LSPNTVKTHVARLYGKLEAARRTDAIRRARELRLIP"
     gene            1716..3167
                     /locus_tag="PLO65_03415"
     CDS             1716..3167
                     /locus_tag="PLO65_03415"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRD27329.1"
                     /translation="MTHEKDAAAIVERLIEEYDRSVSALRGALKTFLTTGERPDPALR
                     ADGAFVYPELQLHWGGENGYPRISRAYARIGMPGHYAATVTRPQMFREYLVEQLALMI
                     QDFGVTLDVGRSTQEIPFPYVLDGGDLALADVSASEIARWFPTTELAHIGDEVADGSW
                     DMVDGETRPLALFDGLRTDFSLARLKHYTGAPAEDVQQYILFTNYHRYVDEFVRWGCE
                     QLKTPGTRFTALSAPGNIVVTSDTPNPEMTIANGPWRRHQMPAYHLIAEGGQGVTLVN
                     IGVGPSNAKTITDHLAVLRPQAWIMIGHCGGLRGSQTIGDYVLAHAYLRDDHVLDQVM
                     PPDIPIPSIAEMQRALYDAAKLVSGESGESLKRRLRTGTVVTTDDRNWELRYTSSALR
                     FNQSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANAFYERAISQ
                     HLQIGIATVDLLRAEGPNLHSRKLRAFDEPPFR"
     gene            3283..3432
                     /locus_tag="PLO65_03420"
     CDS             3283..3432
                     /locus_tag="PLO65_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27330.1"
                     /translation="MFEPLVRDRWTIARERWDEHQRTGAATMSVDEFIAELTEEIEKR
                     QASKA"
     gene            3429..3713
                     /locus_tag="PLO65_03425"
     CDS             3429..3713
                     /locus_tag="PLO65_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD27331.1"
                     /translation="MKRRVEISTLARRDIARLSDFLSPKNPRAWKAAIAAISNWLRSL
                     DQMSERGHFSPDGDLREMIVRFGHAGYVIQYRTAPGEVFVLRIFHSLEER"
     gene            3769..4683
                     /gene="argB"
                     /locus_tag="PLO65_03430"
     CDS             3769..4683
                     /gene="argB"
                     /locus_tag="PLO65_03430"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRD27332.1"
                     /translation="MTDKTEEAGWATAKTLAEALPYIQIYDRETVVIKYGGHAMGQDD
                     VARLFAADAVLLKLMGVHPVVVHGGGPQISAMLDRAGVKSTFVDGLRVTDEATMEVAE
                     MVLSGAINKEIANWITLAGREADVRGVGLSGKDARLITVTKARRTKKDPDSNIEQVVD
                     LGFVGEPQKIDPALIKVLIDSEKDYIPVIAPIGVSDSGQTFNVNADTVAGALAGALKA
                     KRMLLLTDVPGVKDANGDLIRQMTVAEAFDLIRTGVATGGMIPKLETAIAAVDAGVEA
                     VVILDGRRPHAMLVELFTEHGAGTLVKA"
     gene            4680..5339
                     /locus_tag="PLO65_03435"
     CDS             4680..5339
                     /locus_tag="PLO65_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRD27333.1"
                     /translation="MTDLTHIDTWLFDLDNTLYPVESEVMTLVAGRMTDFMERITGLP
                     RDQARAIQKRYYEEHGTTLAGLMANDHGVDPEDFLTHVHDISLDALSPDPALIAGLQR
                     LPGRRLVFTNGSAAHAIRVLERMEMAHLFEDIFHIAAADYIPKPARATFEKMSALHGV
                     SPVGAAFFEDSEKNLAPAAELGMTTVLVGPHAAASTAAFINHRTNDLAAFLNGARVKG
                     T"
     gene            5343..6188
                     /gene="dapD"
                     /locus_tag="PLO65_03440"
     CDS             5343..6188
                     /gene="dapD"
                     /locus_tag="PLO65_03440"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRD27334.1"
                     /translation="MTAVTLADLETEIEAAWEARDGVSPDSRGPVRTAIEETLSLLDS
                     GRVRVAEKIDGDWVVRQWAKKAVLLSFRLNPNTVMRAGTLGGAVGPWWDKVANKFDGW
                     EAPQFEAAGFRAVPGAIVRHGAYVAKNVILMPSFVNIGGYVDEGTMVDTWVTVGSCAQ
                     IGKRVHLSGGVGIGGVLEPLQANPTIIEDDCFIGARSEVVEGVIVGQGSVLSMGVFLT
                     ATTKIVDRKTGEVHRGYVPPYSVVVPGSLPDPHGGPSLSCAVIVKTVDAQTRSKTSIN
                     DLLRD"
     gene            6237..6701
                     /locus_tag="PLO65_03445"
     CDS             6237..6701
                     /locus_tag="PLO65_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRD27335.1"
                     /translation="MQGFGELFSFRGRTSRLTWWRVQLATSALIAALWVLTIFVAMGA
                     GDIAAIPLLLILPVLAVTLATSVRRLHDRDRSAWWLLVFWAAPAACFAAASWLTEQAG
                     DAGPPALAALIAGLAFELWSLVEIGLLSGSRGPNRFGPAPTRQPSRRLRPAT"
     gene            7004..7414
                     /locus_tag="PLO65_03450"
     CDS             7004..7414
                     /locus_tag="PLO65_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27336.1"
                     /translation="MTDYLGQDASRFFYKWGGSFSAVLVRLRGRYDGDLDQFLIHLVF
                     MLTDLASANAVVEARARGAVTVIRRRRGLNVLSLADITRIPRESVRRKLALMVDRSLV
                     VREPDGLYYPGPASDIDGFFYALSPLFWDGVRPG"
     gene            complement(7411..8028)
                     /locus_tag="PLO65_03455"
     CDS             complement(7411..8028)
                     /locus_tag="PLO65_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27337.1"
                     /translation="MLRFEPHLPPPGVFDLFGRSPGRRVRNPFVAGSGARPREVHPER
                     YYLHVRSVSADGFAHLFQFGLVDDRGDVAVNVFVRGRSPVIGQEAGEDEGDPPPVPAI
                     WWDQLEEAMEPCRGAWIIAFGRTLHGSFLPAGVRDDVTGLDCGRARFMKFARRRGLAV
                     APGQIVDLNDARRLVGLPPIRTADAAGRAQGLRELWRWMDLTERS"
     gene            8195..9346
                     /gene="dapE"
                     /locus_tag="PLO65_03460"
     CDS             8195..9346
                     /gene="dapE"
                     /locus_tag="PLO65_03460"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041539765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRD27338.1"
                     /translation="MTQIDALDLARDLIRRPSVTPADLGAMDIVHQALEGLGFACRRM
                     KIGEIENLYARRGTARPNLCFAGHTDVVPVGDAAAWTSPPFEAALADGLLVGRGAVDM
                     KGGIAAFIAAVAAVLARGEPKGSLSFLITGDEEGMAFDGTRKVVEALAAEGEAIDHCI
                     VGEPSSSARLGDMIKIGRRGSINSWITVEGIQGHVAYPHRSANPVTVMVNLLSQLKAR
                     VLDDGYEGFQPSNLEITTIDVGNTASNVIPAEAKARLNIRFNPAHTGAELEAWIAETA
                     RSAGEGFPGKVTVTSVISGEAFLTRPGPFTDVVAAAIAEVTGETTELSTTGGTSDARF
                     IRDLCPVLEFGLVGATMHAVDERVPVSQIRDLQATYERIIERYFETFAA"
     gene            complement(9322..10077)
                     /gene="truA"
                     /locus_tag="PLO65_03465"
     CDS             complement(9322..10077)
                     /gene="truA"
                     /locus_tag="PLO65_03465"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRD27339.1"
                     /translation="MPRYRLTVEYDGRPYRGFQAQGGLPSVQAAIETAVLGFTGETLR
                     IHAAGRTDTGVHATGQVIHLDLAKDWPADVVRDALNAHLVKEAVVVLDAVVVDSDWHA
                     RFSATGRRYLYRILNRKAPPALDRGRVWHVRKPLDAAAMHEAARVLIGHHDFTTFRDM
                     QCQAKSPMKTLDAARVAREGEAVLLTFAARSFLHRQVRSMTGSLVEVGLGRWTAADLA
                     AALEARDRTACGQVAPPDGLYLTDVDYAANVSK"
     gene            complement(10077..>10248)
                     /locus_tag="PLO65_03470"
     CDS             complement(10077..>10248)
                     /locus_tag="PLO65_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27340.1"
                     /translation="HAGTAIAADASLDDGLLIACGTGAVRLLRAQREGKAAQDAADFL
                     RGFPVPAGTALA"
BASE COUNT         1683 a         3542 c         3494 g         1529 t
ORIGIN      
        1 aagaagaagg cgatgttggc caggcccgag atggctcccg cgatcgtcgc ggtgaccgtc
       61 atggtcaggg tgtcgcggtt cttcacatgg gccagttcgt gggccatcac gccgcggatc
      121 tcgcgccggt cgagcatccg cagcaggccg gaggtcgcgg cgacggcggc gtgctgtggg
      181 tcgcggccgg tcgcgaaggc gttcggctgg tcggactcga tgacatgcgc gcgggggcgc
      241 ggcagcccgg cgccgtcggc cagggcgtat acatcggcga cgtagttgcg gaccagcgga
      301 tcgggatggg tctcgtcgac cgccaccgcc cggtacatcg acagcacgat gcggtcggcg
      361 ttccagtagc tgaacaggtt catgcccacc gacaggacca gggcgatcag catgccgccg
      421 gttccgccga tcaggaaccc cgccgtgacg aagatcgccg tcagggcggc gagcaggatg
      481 aaggtgcgga gcgtgttgtt catgccggga gatgtggtcc cggattcacg cgccgcaagg
      541 gccggtcagg accgaaccac cttcatcggc aggaagcggc tgttgcgcag aaggcccgcg
      601 gtgaccagcg agaccaccac ccccaccggg aagatttcga tgaaggtcat cggcaaccgg
      661 aacagcgggc tggcgtactg tgtgcggaag gtttcggtct cggcggtcat cctggcgagc
      721 tcagccgcgg aggcgccttt tgcgcgggcg ccctcgagca tcgcccggct gtagtcgtcg
      781 atgaaggcga agttggtcgc atgcagggtg atctcccagg cgatcacgta gatcacgctg
      841 gccacgacgc tgatgccgag tccgaccagc agcgccggca gaaagcggat caccccgccc
      901 agcgcgcggt cgcgatggtg tttcacgccg acgaaggtga aggtgaaggc cagcagcatg
      961 agcgcgtagc ccgtgaccag cgaatggctg ccgtcgccct ccttgccgaa gatcatcatc
     1021 agattcatcg gcacggcgac gaccaggccg gcggcgaggc cgaagatcag gatggtgcgg
     1081 agcatgggtg ttccccccgt ttgttggaca gccgcaaggg aggcggcatc gacgcctccc
     1141 gacaatcgcc caaaagggtg atttcgcgaa aatcgcccga acgactacgg aatcagccgc
     1201 agctcccgag cccgccggat ggcgtccgtc cggcgcgccg cctcgagctt tccatagagc
     1261 cgggcgacgt gcgtcttgac cgtattgggc gagaggttga gctgccgggc gatctccttg
     1321 ttcgaccggc cggcggccag caggccgagg acctcgtgct cgcggcgggt gacgccaagg
     1381 gccgccaggg ccggggtgtt ggtctcgaag ccgccggtca gcacccgcgg ccggaacagg
     1441 cgagcgccga cccagacgcc caggccgaga aacccggccg cgaccagggc gacccagacc
     1501 tcgccggtat gggtgcgggc caggaggcga tagtccagcc actgcagagc caccgcgccc
     1561 agggccagcg ccaggccata tgtcagcacc gtcttccaca tggtcccgag cctaggatcg
     1621 gttttccgcg cgcgccagta ggtgacttgc gacggttcgg agaaccttgc tcgccgccat
     1681 ctcgccaatt ccgcgccatg ccgctataag cggccatgac acatgagaaa gacgcggccg
     1741 ccatcgtcga gcggcttatc gaagaatacg accggtccgt ttccgccctg aggggcgccc
     1801 tcaagacctt cctgacaacc ggcgaacggc ccgaccccgc gctgcgggcc gacggcgcct
     1861 tcgtctatcc cgagctgcag ctgcactggg gcggcgagaa cggctatccg cggatcagcc
     1921 gcgcctacgc ccgcatcggc atgcccggcc actacgcggc gacggtcacc cgaccgcaga
     1981 tgttccgcga atatctggtc gagcaactgg ccctgatgat ccaggacttc ggcgtgaccc
     2041 tcgacgttgg tcgctcgacc caggaaatcc cgtttcccta cgtgctggat ggcggcgacc
     2101 tggccctggc cgatgtcagc gccagtgaga tcgcccgctg gtttccgacc accgagctgg
     2161 cccacatcgg cgacgaggtg gccgacggtt cctgggacat ggtcgacggc gagacccgcc
     2221 cgctggccct gttcgacggc ctgcgcaccg acttctccct ggcccggctc aagcactata
     2281 cgggtgcgcc ggccgaggac gtgcagcagt acatcctgtt caccaactac catcgctatg
     2341 tcgacgagtt cgtccgctgg ggctgcgagc agctgaagac gccgggcacg cggttcacgg
     2401 ccctgtccgc gccggggaac atcgtggtga cctcggacac gcccaatccc gagatgacca
     2461 tcgccaacgg tccctggcgg cgccaccaga tgccggccta tcacctgatc gccgagggcg
     2521 gacagggcgt gaccctggtc aacatcgggg tggggccgtc gaacgccaag accatcaccg
     2581 accacctggc cgtgctgcgg ccccaggcct ggatcatgat cggccactgc ggtggcctgc
     2641 gcggcagcca gaccatcggc gactatgtgc tggcccacgc ctatctgcgc gacgaccatg
     2701 tgctcgacca ggtgatgccg ccggacattc cgatcccctc gatcgccgag atgcagcggg
     2761 ccctgtacga cgccgcgaag ctggtcagcg gcgaaagcgg cgaaagtctc aagcgccgcc
     2821 tgcgcacggg caccgtggtg accaccgacg accggaactg ggagctgcgc tacacctcca
     2881 gcgccctgcg cttcaaccag agccgggccg tggccatcga catggaaagc gcgaccatcg
     2941 ccgcccaggg ttaccgtttc cgggtgccct acggcacgct gctgtgcgtt tcggacaagc
     3001 cgctgcacgg cgagatcaag ctgcccggcc aggccaacgc cttctacgag cgggccatca
     3061 gccagcacct gcagatcggc atcgccacgg tggacctgct gcgggccgag ggtcccaacc
     3121 tgcactcgcg caagttgcga gccttcgatg agccgccgtt tagatagagc ttttcgtggc
     3181 ctgtcggtcc gcgtatagtg acccatggcc ggcccacgaa agtcccctga tgaagacacg
     3241 ccgcatacgg cgcaacagcc tggcctggag gaggctggcg ctgtgttcga gcctcttgtt
     3301 cgcgatcgct ggacgatcgc ccgggagcgg tgggacgagc accagagaac gggcgccgct
     3361 acgatgtcgg tcgatgagtt cattgctgag ttgaccgagg agatcgaaaa gcggcaggcc
     3421 tccaaggctt gaagcggcgc gtcgaaatct cgaccctggc ccgacgtgat atcgcgcgcc
     3481 tatctgactt tctgagcccg aagaatcccc gagcatggaa ggccgccatc gcggcaatca
     3541 gcaactggtt gaggtcgctc gaccagatgt ccgagcgtgg tcatttctcg ccggatggcg
     3601 acctgcgcga aatgatcgtg cgtttcggcc acgccggcta cgtcatccag taccggaccg
     3661 cgccggggga agtgttcgtc ctccgcatct tccacagcct tgaagagcgg tgacacaccg
     3721 gcccccatcc gctatcgcac ggcggcgaat ttccgggagc tcagcgcctt gaccgacaag
     3781 accgaggaag ccggctgggc gaccgcgaag accctcgccg aagccctgcc ctacatccag
     3841 atctatgacc gcgagacggt ggtgatcaaa tacggcggcc acgccatggg tcaggacgac
     3901 gtggcccggc tgttcgcggc cgacgccgtg ttgctcaagc tgatgggcgt gcatccggtg
     3961 gtggtccacg gcggcgggcc gcagatctcg gccatgctcg accgtgccgg cgtcaagtcg
     4021 accttcgtcg acggcctgcg ggtgacggac gaggcgacca tggaggtggc cgagatggtc
     4081 ctctccggcg cgatcaacaa ggagatcgcc aactggatca ccctggccgg ccgcgaggcg
     4141 gacgtgcgcg gcgtgggcct gtcgggcaag gacgcgcggc tgatcaccgt gaccaaggcg
     4201 cggcggacga agaaggaccc cgacagcaac atcgagcagg tcgtcgatct gggcttcgtc
     4261 ggcgaaccgc agaagatcga cccggccctg atcaaggtgc tgatcgactc ggagaaggac
     4321 tacatcccgg tcatcgcccc gatcggcgtg tcggactcgg gccagacctt caacgtcaac
     4381 gccgacacgg tcgccggcgc cctggccggg gcgctgaagg ccaagcgcat gctgctgctg
     4441 accgacgtgc cgggggtcaa ggacgccaac ggcgacctga tccgccagat gaccgtggcc
     4501 gaggcgttcg acctgatccg caccggtgtc gccaccggcg gcatgattcc caagctcgag
     4561 acggccatcg ccgcggtcga cgccggggtc gaggcggtgg tcatcctcga cggccggcgg
     4621 ccgcacgcca tgctggtcga gctgttcacc gaacacggcg ccggaaccct ggtgaaggcg
     4681 tgaccgacct cacccacatc gacacctggc tgttcgatct cgacaacacc ctctacccgg
     4741 tcgagagcga ggtcatgacg ctcgtcgccg gccggatgac cgacttcatg gagcgcatca
     4801 ccggcctgcc gcgcgaccag gcccgggcga tccagaagcg ctactacgag gaacacggca
     4861 cgaccctggc cgggttgatg gccaacgacc acggcgtcga tccggaagac ttcctcaccc
     4921 atgtgcatga catctcgctc gacgccctgt cgcccgaccc ggccctgatc gccgggctgc
     4981 agcggctgcc gggtcggcgg ctggtgttca ccaacggctc ggcggcccac gccatccggg
     5041 tgctcgagcg aatggagatg gcccacctgt tcgaggacat cttccacatc gccgccgccg
     5101 actacattcc caagccggcc cgggcgacct tcgagaagat gagcgccctg catggcgtca
     5161 gccccgtcgg cgcggccttc ttcgaggaca gcgagaagaa cctcgccccg gcggcggagc
     5221 tgggcatgac cacggtgctg gtcgggccgc acgccgccgc ctcgacggcc gccttcatca
     5281 accatcgcac caacgacctc gccgccttcc tcaacggggc gcgggtgaag gggacctgac
     5341 atatgaccgc cgtgacgctc gccgacctgg aaaccgagat cgaggccgcc tgggaggccc
     5401 gcgacggcgt ctcgccggac agccgcgggc cggtgcgcac ggcgatcgag gagaccctct
     5461 cgctgctcga ttccggtcgc gtccgcgtgg ccgagaagat cgatggcgac tgggttgtcc
     5521 ggcagtgggc caagaaggcc gtgctgctgt ccttccgcct caaccccaat accgtcatgc
     5581 gcgccggaac cctgggcggg gcggtcggac cctggtggga caaggtggcc aacaagttcg
     5641 acggctggga ggcgccgcag ttcgaggccg ccggcttccg cgcggtgccc ggcgccatcg
     5701 tccgccacgg cgcctatgtc gcgaagaacg tcatcctcat gccctcgttc gtgaacatcg
     5761 gcggctatgt cgacgagggg accatggtcg acacctgggt cacggtcggc agctgcgccc
     5821 agatcggcaa gcgggtgcac ctctccggcg gcgtcggcat cggcggggtg ctggagccat
     5881 tgcaggccaa cccgacgatc atcgaggacg actgcttcat cggcgcccgc tcggaggtcg
     5941 tcgagggcgt catcgtcggc cagggcagcg tgctgtcgat gggcgtgttc ctgaccgcga
     6001 caaccaagat cgtcgaccgc aagaccggcg aggtgcaccg cggctacgtc ccgccctaca
     6061 gcgtggtggt gcccggcagc ctgcccgatc cccatggcgg cccgagcctg tcctgcgcgg
     6121 tgatcgtcaa gacggtcgac gcccagaccc gctcgaagac ctcgatcaac gacctgctgc
     6181 gggactagcc gggctccggc gttcgaccta gggtcgcggg cggggaggtc ctcgtcatgc
     6241 agggtttcgg tgaactgttc tccttccggg gccgcacatc ccgcctgacc tggtggcgcg
     6301 ttcagctcgc cacgtcagcg ctgatcgcgg cgctctgggt gctgaccatc ttcgtggcca
     6361 tgggcgcggg cgacatcgcc gcgattcccc tgctgctgat cctgccggtg ctggcggtga
     6421 ccctggcgac gtccgtgcgg cgactgcatg atcgcgacag gagcgcctgg tggctgctgg
     6481 tcttctgggc cgcgccggcg gcctgtttcg ccgcggccag ctggctgaca gagcaagcgg
     6541 gggatgccgg cccgccggcc ctggcggcgc tgatcgcggg cctggccttc gaactctggt
     6601 cgctggtgga gatcggccta ctgtccggat cgaggggtcc caaccggttc ggcccggcgc
     6661 cgacgcgtca gccgtcgcga cggctccggc cggcgacctg acgcatcccc ctcttcatcc
     6721 accgccgtcc acagaaaagg accacccagg cgcgttccag cgttgaatgc ggctgcggct
     6781 tgatgtctct gccgggtccg ggcggctggg aagtggcggg tcgatcttga ggcgatctat
     6841 gattaatacg aaattcgcca aaacaatcga ttacacgatt tcatcgaacg actacgcgga
     6901 gccgaccaga attaatctta acgggttcat cggtgttgcg gcgaagacag tcccgccgcc
     6961 agtcgagacc cggaccgcgc cgccgactcc gaccgaccgt tgggtgaccg actacctggg
     7021 acaggacgcc tcgcgcttct tctacaaatg gggcggctcg ttttccgccg tgctggtccg
     7081 cctgcgcggt cgctacgacg gcgatctcga ccagttcctg atccacctgg tgttcatgtt
     7141 gaccgacctg gcctcggcca acgccgtggt ggaggcgcgg gcacgcggcg cggtcaccgt
     7201 gatccggcgg cggcgggggc tgaacgtcct gagcctggcg gacatcaccc ggatcccgcg
     7261 cgagagcgtg cggcgaaagc tggccctgat ggtcgaccgc agcctggtcg ttcgggagcc
     7321 ggacggactc tactatccgg gccccgccag cgacatcgac ggcttcttct atgcgttgag
     7381 cccgctgttc tgggacggcg tacggcccgg ctaggaccgc tccgtcagat ccatccagcg
     7441 ccaaagctcg cgcaggccct gcgcccgccc ggccgcgtcg gcggtccgga tcggaggcag
     7501 gccgaccagg cgacgggcgt cgttcaggtc aacgatctgg cccggcgcca cggccagacc
     7561 ccgccgccgc gcgaacttca tgaaccgcgc gcggccgcag tcgaggccgg tcacgtcgtc
     7621 gcgcacgccg gccggcagga acgagccgtg aagggtgcgg ccgaaggcga tgatccaggc
     7681 gccgcgacac ggctccatcg cctcctcgag ctggtcccac cagatggctg gcacgggagg
     7741 agggtcgccc tcatcctcgc cggcctcctg gccaatgacc ggactgcggc cgcgcacgaa
     7801 gacattgacc gccacgtcgc cgcgatcgtc caccaggccg aactggaaca gatgggcgaa
     7861 gccgtcggcc gagaccgacc gcacatgcag gtagtagcgc tcgggatgga cctcccgcgg
     7921 ccgtgcgccg gatccggcga cgaacggatt gcggacgcgc cggcccgggg agcggccgaa
     7981 aaggtcgaac acgcccggag gcggcaggtg aggttcaaaa cgcagcatgg cgtcctcctg
     8041 aagctgaccg gccgatctgg ccggggcgga ggcccgatgt gaatgaaggg gcggcccgtt
     8101 ccgggtggcc cggaatgggc tatcccgcgc tggcggaggg ccggaaccgc tctggggcct
     8161 tgcgcgaacg ccgcccggcc ctaaacggag tccgatgacc cagatcgacg ccctcgacct
     8221 cgcccgcgac ctgatccgcc gtccgtcggt gacacccgcc gacctcggcg cgatggacat
     8281 cgttcaccag gccctcgagg gcctgggctt cgcctgtcgg cggatgaaga tcggcgagat
     8341 cgagaacctc tacgcccggc gcgggaccgc tcgcccgaac ctctgcttcg ccggccacac
     8401 cgatgtggtg ccggtcggcg acgccgccgc ctggaccagc cccccgttcg aggccgccct
     8461 ggctgacggg ctgctggtcg ggcgcggcgc cgtcgacatg aagggcggca tcgccgcctt
     8521 tatcgccgcg gtggccgccg tgctggcccg cggcgagccg aagggctcgc tcagcttcct
     8581 gatcaccggc gacgaggaag gcatggcctt cgacggcacg cgcaaggtgg tcgaggcgct
     8641 ggccgccgag ggggaggcga tcgaccactg catcgtcggc gaaccgtcca gctcggcccg
     8701 gctgggcgac atgatcaaga tcggccgccg gggcagcatc aacagctgga tcacggtcga
     8761 gggcatccag gggcatgtcg cctatcccca ccgctcggcc aatccggtca cggtgatggt
     8821 caacctgctg tcgcagctga aggcccgggt gctggatgac ggctacgaag ggttccagcc
     8881 gtcgaacctc gagatcacca ccatcgacgt cggcaacacc gcctcgaatg tcatcccggc
     8941 cgaggcgaag gcccggctga acatccgctt caacccggcc cacaccggcg cggagctgga
     9001 ggcctggatc gccgagacgg cgcggtccgc cggcgaaggg ttccccggca aggtcaccgt
     9061 cacctcggtg atcagtggcg aggccttcct gacccggccc ggcccgttca ccgatgtggt
     9121 cgcggccgcg atcgccgagg tcacgggcga gaccacggag ctgtcgacga cgggcggcac
     9181 gtccgacgcc cggttcatcc gcgacctttg ccccgtgctc gagttcggcc tggtcggcgc
     9241 gaccatgcac gcggtcgacg agcgcgtgcc cgtcagtcag atccgcgacc tgcaggcgac
     9301 ctacgagcgg atcatcgagc gctacttcga aacgttcgcg gcgtagtcga cgtcggtcag
     9361 gtaaaggccg tccggcggcg ccacctggcc gcaggccgtg cggtcccgcg cctcgagcgc
     9421 cgccgccaga tccgccgctg tccagcgccc gaggccgacc tcgaccagtg acccggtcat
     9481 cgaccgaacc tgacggtgca ggaacgaacg ggccgcgaaa gtcagcagca ccgcctcgcc
     9541 ttcgcgggcc acgcgcgccg catcgagcgt cttcatcggc gacttcgcct ggcactgcat
     9601 gtcgcggaaa gtggtgaagt cgtgatggcc gatcaggacc cgggcagcct catgcatggc
     9661 cgccgcgtcc agcggcttgc gcacgtgcca gacccggccg cggtcgagcg ccggcggcgc
     9721 cttgcgattg aggatgcggt agagatagcg ccgcccggtg gccgagaagc gggcgtgcca
     9781 gtcgctgtcg acgacgacgg cgtcgagcac aacgacagcc tccttcacca gatgggcgtt
     9841 gagcgcgtcg cgcacgacat ccgccggcca gtccttcgcg aggtccaggt ggatcacctg
     9901 tcccgtggcg tggacgcccg tgtcggttcg accggccgcg tggatgcgca gggtctcgcc
     9961 cgtgaagccc agcacggccg tctcgatggc cgcctgcacg ctcggcaggc caccctgggc
    10021 ctggaagccg cgatagggcc ggccgtcata ttcgactgtc aggcgatagc ggggcatcag
    10081 gccagcgccg tcccggcggg gaccgggaag ccgcgcagga agtcggcggc gtcctgggcc
    10141 gccttgccct cgcgctgggc ccgcagcagc cgcaccgcgc cggtcccgca ggcgatcagc
    10201 agcccgtcgt ccaggctcgc gtcggcagcg atcgccgtcc cggcgtgc
//
LOCUS       DAORNX010000047        10192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4886_length_10192_cov_8.886061, whole genome shotgun sequence.
ACCESSION   DAORNX010000047 DAORNX010000000
VERSION     DAORNX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_10192_cov_8.886061"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLO65_03475"
     CDS             complement(<1..217)
                     /locus_tag="PLO65_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HRD27341.1"
                     /translation="MSDENDNGRPARPSLTLKPRTGSVNAGVVKQTFSHGRSKTVVVE
                     TKRVRTHAPGGGANLAGPSAAEKRSTYE"
     gene            complement(306..935)
                     /locus_tag="PLO65_03480"
     CDS             complement(306..935)
                     /locus_tag="PLO65_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRD27342.1"
                     /translation="MQTPATHAQASRERRDIVSGEVMAEERLIRFVAGPDGVVTPDLA
                     RKLPGRGLWVAADRTSLETAVKKGLFSRAARTRLTAPAALPDLVEALLLRRLLDGLGL
                     ARRSGDLTSGFEKVMSSIRGGKAAWLIEASDGAADGRRKLLGAIHSSPSPPRLFGLFS
                     SEELGLALGGENVIHTAFLAGRASGRWTSDAQRLSGFRPLLPESWREEP"
     gene            complement(1031..2695)
                     /gene="nusA"
                     /locus_tag="PLO65_03485"
     CDS             complement(1031..2695)
                     /gene="nusA"
                     /locus_tag="PLO65_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRD27343.1"
                     /translation="MATGISANRLELLQIADAVAREKGIEKEIVIASIEEAIQKAARS
                     RYGAEHDIRVNIDPKTGETSIKRYLTVVPDDAEIDEETLGVIRLGDALAIDRDAFVGQ
                     VTEEELPPFEFGRVQTQMARQVVTGKVREAERERQFEEFKDRVGEIVNGSVKRVEYGN
                     VIVDLGRGEGIMRRDQSIPRENFNIGDRIRCYIYDVRRETKGPQIMLSRAHGGFMAKL
                     FAQEVPEVYDGVIEIRAVARDPGSRAKMAVVSNDSSIDPVGACVGMRGSRVQAVVAEL
                     QGEKIDIIQWNPDEATFIVNALAPAEVSKVVMDEEDERVEVVVPDEQLSLAIGRRGQN
                     VRLASQLTGWQIDIMTESQESERRQKQFAETTALFQEALDVDEVIAQLLATEGFISVE
                     DVAYVEPHEIAAIEGFDEDTADELQARARDFLDKEAAAQDARRLELGVEDAVLEIDGV
                     TLPMAVALGEGDVKSVEDLAGLVPDDLRGWFENRDGERVREPGILEAFALSPEDAEAL
                     IMRARVAMGWIEAPPEPEYEPEYESEGLDNPEDFDLAAAEGEARDA"
     gene            complement(2714..3262)
                     /gene="rimP"
                     /locus_tag="PLO65_03490"
     CDS             complement(2714..3262)
                     /gene="rimP"
                     /locus_tag="PLO65_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRD27344.1"
                     /translation="MRARTAEDARLLELLDPVAEAAGFEIVRLRLMAGKERRRLQVMA
                     ETPDGDMTVEGCTVLSRAFSEVLDAADPISGEYTLEVSSPGIDRPLTRLKDFDTWEGF
                     EARIELDRLADNRKRFKGELAGTEDGQVGLNLEGEGETTLYIPFDWILEAKLVLDDKL
                     MKHGAEERAARLQTDSDIDQET"
     gene            complement(3385..3882)
                     /locus_tag="PLO65_03495"
     CDS             complement(3385..3882)
                     /locus_tag="PLO65_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HRD27345.1"
                     /translation="MSFSVHQASAPVFTRALANLSAMIDKALAAGVDEATLLEARLAP
                     DMHPFPRQIQMASDSAKGAVARLAGIEPPSMPDTETTIAQLKDRIAATIAYVNGVDAS
                     ALDGAEDREIVLKFPQGEMKFAGCDFLTGFALPNFFFHITIAYALMRANGAPIGKMDF
                     LAGAR"
     gene            complement(4008..4685)
                     /gene="trmB"
                     /locus_tag="PLO65_03500"
     CDS             complement(4008..4685)
                     /gene="trmB"
                     /locus_tag="PLO65_03500"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRD27346.1"
                     /translation="MPVTPQQPHGALRSYGRIKARPIKARQAEVLATVGPTIEIPQGP
                     FDPRALRPDAREIWLEIGFGGGEHMAGQAERSPDVLIIGVEPFLNGVASAVRHVDDRG
                     LTNVRIHQGDAREVAARLPDACLDRVFILFPDPWPKSRHHKRRIIQPEFIVELARLLK
                     PGGGLRFATDWADYADWTRQRVPASGLFVPVEADTALPPADHLTTRYEEKRLGDCAPV
                     FLDFVRV"
     gene            complement(4796..6019)
                     /gene="metK"
                     /locus_tag="PLO65_03505"
     CDS             complement(4796..6019)
                     /gene="metK"
                     /locus_tag="PLO65_03505"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRD27347.1"
                     /translation="MSRSSYLFTSESVSEGHPDKVADRISDTVVDAFLTADPEARVAC
                     ETLVTTNRIVLAGEVRAGKPNGDKAANKAFTKDIIKSLEPKVRAAIKDIGYEQKGFHW
                     EKAKYACYLHGQSAHIAQGVDASDKKDEGAGDQGIMFGYASTETPEFMPATLQYSHNI
                     LRRLAELRHSGELHELEPDAKSQVTLEYDANGRPTRVVSIVVSHQHKKKIDGKAPTSR
                     RVLDLIRPHVLSVFPDGLVNKKTQWLINPTGRFEIGGPDGDAGVTGRKIIVDTYGGAA
                     PHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGLADRCTIQIAYAIGVARPVSFHVD
                     MHGTGKVDPALLEKVLPDLINGATPRAIREHLGLNKPIYARTAAYGHFGRTPDNEGGF
                     SWEKTDLVPELKKLA"
     gene            complement(6112..6552)
                     /locus_tag="PLO65_03510"
     CDS             complement(6112..6552)
                     /locus_tag="PLO65_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27348.1"
                     /translation="MTTTTETDRSPNPVDLHVGGRIRMRRKLLGVSQERLADDLGLTF
                     QQVQKYERGANRVSASKLYEIAKSLQTSIAYFFEGLVDPSTARAEGVAESGAEPFIHD
                     FLMTPEGLELAALFPKIRRGRVRRRILDLVRSMAEDDENEPVEA"
     gene            complement(6597..>7869)
                     /gene="lnt"
                     /locus_tag="PLO65_03515"
     CDS             complement(6597..>7869)
                     /gene="lnt"
                     /locus_tag="PLO65_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRD27349.1"
                     /translation="ACLAYRWTGAKGVARVLVFVGALAACEWLRGHVLTGFPWNLPGE
                     TWKAGSAMSQTAALVGAYGLTWVTLALVVPVAVWREGRAGRIAVGLAAAALAGLWLFG
                     AVRSMDRPAPSGQRALTVRMVQADIKQSAKYDPAEFQRIVDRYVALTSRPYPDGRVAD
                     IVIWPEGAIPDAIDNYLAPGTWTRAAIEGALRPGQMLLVGAYRVEGTQGRYRYYNSLV
                     AARRTAGGFEPAGVYDKYRLVPFGEFLPLDAWMEKLGVKTLVHVGDGFTPGPPPRPMT
                     IPGLPAFQPLICYEGLYPGYTRTGARASGARPDFIVNVSNDAWFGVTSGPLQHLNLSS
                     YRAIEEGIPILRATPTGVSAMIDARGAATGHVLRLGESDVIDGHIPWQRFETVYRRFG
                     DLPFLLMLLISGLVYLPKRVVWSLKSQRNRN"
     assembly_gap    7870..8045
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8190..9071)
                     /locus_tag="PLO65_03520"
     CDS             complement(8190..9071)
                     /locus_tag="PLO65_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRD27350.1"
                     /translation="MAHSDDASSPETPRPRRGFWSLFGRVRHGLAGEPVAEIEPTTQA
                     GETLAAQARAFQTLTVDDVMTPRADIVAVEFNTPFEALVAQFSETEHSRLPVYRGTLD
                     DPIGFVHIKDVFKLLASKGRRPAGVLKRLRRPAPGVPGSMRAATLLAQMQKTRVHLAL
                     VIDEYGGTEGLVTLEDLIEAVVGDIADEHDDAAVTGLRVMPDGVIEASARALLEDLEA
                     VLGEDQLGDGDHEEDIDTVGGLVTAIAGRVPRRGDVVPLSDRYDIEVIAADPRRVKRV
                     RFRPRPLPTTAEETSAA"
     gene            complement(9077..9511)
                     /gene="ybeY"
                     /locus_tag="PLO65_03525"
     CDS             complement(9077..9511)
                     /gene="ybeY"
                     /locus_tag="PLO65_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRD27351.1"
                     /translation="MLGIDIEIEDEAWVEALPAAEHLALQAAEAATAEGGLTVLLTDD
                     ATVADLNERFRGKSGPTNVLSFPAPANPEGHLGDIALAYGVCAREAAQQGKTLAHHLQ
                     HLVAHGVLHLVGYDHQTETEAEEMEALERLILAGLGVADPYA"
     gene            complement(9512..>10192)
                     /locus_tag="PLO65_03530"
     CDS             complement(9512..>10192)
                     /locus_tag="PLO65_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD27352.1"
                     /translation="HKPAAPGAPRRGPVSPKTATQARYMEALGSHDLVFGLGPAGTGK
                     TFLAVAHGAAMLMRGDIDRLIVTRPAVEAGERLGFLPGDLTEKVDPYMAPVWEALTQL
                     MGADQLRRRRDKGEIEVAPIAFMRGRTLAHAFVIVDEAQNTSRLQMKMVLTRLGEGSR
                     MAVTGDPSQIDLLNPADSGLAHAVAILEDVEGVSVTRFTAADVIRHPMVARIVRAYDA
                     DAAKRTGQ"
BASE COUNT         1475 a         3383 c         3515 g         1643 t
ORIGIN      
        1 gctcgtaggt cgagcgcttt tcagcggccg agggaccggc gaggttggcg ccgccgccgg
       61 gcgcatgcgt gcgcacgcgc ttggtctcga ccaccaccgt cttcgaccgg ccgtgactga
      121 aggtctgctt gaccacgccc gcgttcaccg agcccgtacg gggcttcagg gtgagggagg
      181 ggcgggcggg ccggccgttg tcgttctcgt cgctcatgcg tctcgcttaa ctcacagccg
      241 gcggatccgg cttcaattcg ttcagttcaa accgcgaagg gaccgtggcg tcgccgcccg
      301 gtccactacg gctcctcgcg ccaactctcg gggagaagcg ggcggaagcc tgatagccgc
      361 tgggcgtccg atgtccagcg tccggaggct cgccccgcaa ggaaggcggt gtgtatcaca
      421 ttctcgcccc ccaaggccaa acccaattcc tcagaggaaa aaaggccgaa cagacggggc
      481 ggtgaaggac ttgaatggat cgcgccgagc agcttgcgac ggccgtccgc cgcgccgtcc
      541 gacgcctcga tcagccaggc cgccttgccg ccgcggatgg acgacatgac cttctcgaac
      601 cccgaggtaa ggtctccgga gcgtcgcgca aggcccaggc cgtcgagaag tcgcctgagc
      661 agcagggcct cgaccaggtc ggggagagcc gccggcgcgg tcagcctcgt cctggcggcc
      721 cgggagaaca ggcccttctt caccgccgtc tcgaggctgg tccggtcggc cgccacccac
      781 aggccgcggc ccggcagctt gcgcgccagg tcgggcgtga ccaccccgtc cggtccggcg
      841 acgaaccgga tcaaccgctc ctcggccatg acctcgccgg agacgatgtc gcggcgctcg
      901 cgggaggcct gggcgtgggt ggcgggagtc tgcataggga gtagggcgta gggaggtagg
      961 gagtagcggc cctggcgaag gcgggcgcag ctcggctacg ccctacctcc ccaagcccta
     1021 ctccctgccc tcacgcgtcc cgcgcctcgc cttcggcggc ggccaggtcg aagtcctcgg
     1081 ggttgtcgag gccctcggac tcgtactccg gctcgtactc gggctcgggc ggcgcctcga
     1141 tccagcccat ggcgacgcgg gcgcgcatga tcagcgcctc ggcgtcctcg ggactcaggg
     1201 cgaaggcttc gaggatgccc ggttcgcgga cccgctcgcc gtcgcggttc tcgaaccagc
     1261 cgcgcaggtc atcgggcacc aggccggcca ggtcctcgac ggacttcaca tcgccctcgc
     1321 cgagcgccac ggccatgggc agggtcacgc cgtcgatctc gagcacggcg tcctcgacgc
     1381 ccagttcgag gcggcgggcg tcctgggcgg cggcttcctt gtcgaggaag tcgcgcgcgc
     1441 gggcctgcag ctcgtcggcg gtgtcctcgt cgaagccctc gatggccgcg atctcgtgcg
     1501 gctcgacata ggccacgtcc tcgaccgaga tgaagccctc ggtggccagc agctgggcga
     1561 tgacttcgtc cacgtccagg gcttcctgga acagggcggt ggtctcggcg aactgcttct
     1621 ggcgccgctc gctctcctgc gactccgtca tgatgtcgat ctgccagccg gtcagctggg
     1681 acgcgagtcg gacgttctgg ccgcggcggc cgatggccag cgacagctgc tcgtcgggca
     1741 ccaccacctc gacgcgctcg tcctcctcgt ccatgacgac cttggacacc tcggccgggg
     1801 ccagggcgtt gacgatgaag gtcgcctcgt ccgggttcca ctggatgatg tcgatcttct
     1861 cgccctgcag ttcggcgacc accgcctgca cgcgcgagcc gcgcatgccg acgcaggcgc
     1921 cgaccgggtc gatgctcgag tcgttggaga cgacggccat cttggcgcgg ctgcccgggt
     1981 cgcgggccac ggcgcggatc tcgatcacgc cgtcgtacac ctccggcact tcctgggcga
     2041 acagcttggc catgaagccg ccgtgggcgc ggctgagcat gatctgcgga cccttggtct
     2101 cgcgacggac gtcgtagatg tagcagcgga tgcgatcgcc gatgttgaag ttctcgcgcg
     2161 ggatcgactg gtcgcggcgc atgatgccct cgccgcggcc cagatcgacg atgacgttgc
     2221 cgtactcgac gcgcttgacg ctgccgttga cgatctcgcc gacgcggtcc ttgaactcct
     2281 cgaactggcg ctcgcgctcg gcctcgcgga ccttgccggt gaccacctgg cgggccatct
     2341 gggtctgcac ccggccaaac tcgaacggcg gcagctcctc ctcggtgacc tggccgacga
     2401 aggcgtcgcg gtcgatcgcc agggcgtcgc cgagacggat gacgccgagc gtctcctcgt
     2461 cgatctcggc gtcgtccggc accacggtga ggtagcgctt gatcgaggtc tcgccggtct
     2521 tggggtcgat gttgacccgg atgtcgtgct cggcgccgta gcggctgcgg gccgccttct
     2581 ggatcgcctc ctcgatggag gcgatgacga tctccttctc gatgcccttt tcgcgggcga
     2641 ccgcgtcagc gatctgcagg agctcgagcc ggttggcgga aatgccggtg gccatggtgg
     2701 ggattccctg gggttaggtc tcttggtcga tgtcggagtc ggtttgcaga cgggcggcgc
     2761 gttcctcggc gccgtgcttc atcagcttgt cgtcgagcac cagcttggcc tcgaggatcc
     2821 agtcgaacgg gatgtagagg gtggtctcgc cctcgccctc gaggttgagt ccgacctggc
     2881 cgtcctcggt ccccgccagc tcgcccttga agcgcttgcg gttgtcggcc aggcggtcga
     2941 gctcgatgcg ggcctcgaaa ccttcccagg tgtcgaagtc cttcagccgg gtcaggggcc
     3001 ggtcgatgcc ggggctcgag acctccagcg tatattcacc gctgatcggg tcggccgcgt
     3061 cgagcacctc ggaaaaggcc cgcgacagca cggtgcagcc ctcgacggtc atgtcgccgt
     3121 cgggggtttc ggccatcacc tgcaggcggc ggcgttcctt gccggccatc aggcgcaggc
     3181 ggacgatttc gaacccggcc gcctcggcga ccggatcgag caactcgagc agtcgggcgt
     3241 cttccgccgt ccgtgcgcgc atcggtgcca aacccaaaca aaaagcggcg acctctcggc
     3301 cgccgctcga atgcgccatc cagcgcgttc aaacgatgtg aggcccttat aggccccttc
     3361 atcgcccttg tcagccccgg attctcagcg ggccccggcc aggaaatcca tcttgccgat
     3421 cggcgcgccg ttggcccgca tcagggcgta ggcgatggtg atatggaaga agaagttcgg
     3481 cagggcgaag ccggtcagga agtcgcagcc ggcgaacttc atctcgccct gcgggaactt
     3541 cagcacgatc tcgcggtcct ccgcgccgtc gagcgccgag gcgtcgacgc cgttcacata
     3601 ggcgatggtc gcggcgatcc ggtccttcag ctgggcgatg gtggtctcgg tgtcgggcat
     3661 cgacggcggt tcgatcccgg cgagccgggc cacggcgccc ttggcgctgt cggaggccat
     3721 ctggatctgc cgcgggaagg gatgcatgtc cggcgcgagg cgggcctcga gcagggtcgc
     3781 ctcgtcgacg ccggcggcca gcgccttgtc gatcatggcc gagagattgg ccagggcgcg
     3841 ggtgaagacg ggcgcggagg cctggtggac ggagaagctc atggtcgggt gttccggatt
     3901 ctgggtgaac agcgtgaggc gctcacatcg gggttcccgg ggcggaaatc cagagtacgc
     3961 ggcctgttca gggttgaaag acttgttcgg ccttccctga ccgatcctca gacccgcacg
     4021 aaatccaaga acaccggcgc gcagtcgccg agccgctttt cctcgtagcg ggtggtcagg
     4081 tggtcggcgg gtggaagggc ggtgtcggcc tcgaccggca cgaacaggcc gctcgccggg
     4141 acgcgctggc gcgtccagtc ggcatagtcg gcccagtcgg tggcgaaccg caggcccccg
     4201 cccggcttca gcaatcgcgc cagttcgacg atgaattcgg gctggatgat ccgccgcttg
     4261 tgatgccgcg acttgggcca ggggtcggga aacaggatga acacccggtc gagacaggcg
     4321 tccggcagcc gggccgcgac ctcgcgcgcg tcgccctggt ggatgcggac attggtcagg
     4381 cctcgatcgt cgacatggcg cacggcgctg gccacgccgt tgaggaacgg ctcgacaccg
     4441 atgatcagga catcgggcga gcgctcggcc tgtccggcca tgtgctcgcc gccgccgaag
     4501 ccgatctcca gccatatctc gcgggcgtcc ggccgcaacg cccgggggtc gaacggcccc
     4561 tggggaatct cgatcgtcgg cccgaccgtg gccagcacct cggcctgccg ggccttgatc
     4621 ggccgggcct tgatgcggcc atagctgcgc agggcgccgt ggggctgttg cggggtgacg
     4681 ggcatggggg ctcaataaca gaaaagggac atgcacttca gtgcgtgtcc cttcatccgg
     4741 atccgtggga ggcgtgggaa ggggacacgc actgacgtgc atgtccccgt cggcttcagg
     4801 ccagcttctt gagctccgga accaggtcgg tcttctccca gctgaagcca ccttcgttgt
     4861 ccggcgtgcg gccgaagtgg ccgtaggcgg cggtgcgggc gtagatcggc ttgttcagcc
     4921 ccaggtgctc gcggatcgcc cgcggggtcg cgccgttgat caggtccggc aggacctttt
     4981 ccagcagcgc cggatcgacc ttgcccgtgc cgtgcatatc gacatggaac gacaccggcc
     5041 gcgccacgcc gatggcgtag gcgatctgga tcgtgcagcg gtcggcgagg ccggcggcga
     5101 ccacgttctt ggccaggtag cggcaggcgt aggccgccga gcggtcgacc ttggtcgggt
     5161 ccttgccgct gaaggcgccg ccgccgtgcg gggccgcgcc gccataggtg tcgacgatga
     5221 tcttgcggcc ggtgacgccg gcgtcgccgt cggggccgcc gatctcgaag cggccggtcg
     5281 gattgatcag ccactgggtc ttcttgttca ccaggccgtc ggggaagacg gacaggacgt
     5341 gcggacggat caggtccagc acgcgccggc tggtcggggc cttgccgtcg atcttcttct
     5401 tgtgctggtg cgagacgacg atcgagacca cccgcgtcgg ccggccattg gcgtcatact
     5461 ccagcgtgac ctggctcttg gcgtccggct cgagctcgtg cagctcgccc gaatggcgca
     5521 gctcggccag gcggcgcagg atgttgtggc tgtactgcag ggtggccggc atgaattccg
     5581 gcgtctcggt cgaggcgtag ccgaacatga tgccctggtc gccggcgccc tcgtccttct
     5641 tgtcgctggc gtccacgccc tgggcgatgt gggccgactg gccgtgcagg tagcaggcgt
     5701 atttcgcctt ctcccagtgg aagcccttct gctcgtagcc gatatccttg atcgccgccc
     5761 gcaccttggg ctcaagcgac ttgatgatgt ccttggtgaa cgccttgttg gccgccttgt
     5821 cgccattcgg cttgccggcg cgcacctcgc ccgccagcac gatccggttg gtcgttacca
     5881 gggtttcgca ggcgacccgc gcctccgggt cggccgtgag gaaggcatcg acgacggtgt
     5941 cactgatccg gtcggcgacc ttgtcggggt gcccctcgga aacgctttca ctggtgaaga
     6001 ggtaggagga tcgactcacg gcttggctcc gaacacgacg aagccgcccg gaacgagcgg
     6061 cttcgcagaa cctataaaga tatccttata tccgcaagcg gcgcgaggcc gtcaggcctc
     6121 gaccggctcg ttttcatcat cctcggccat cgaacggacc aggtcgagga tgcggcggcg
     6181 cacgcggccc cggcggatct tcggaaacag cgccgcgagt tccaggcctt cgggggtcat
     6241 caggaaatca tggatgaacg gttcggcccc gctttcggcc accccttcgg cgcgagccgt
     6301 cgacgggtcg accaggcctt cgaagaaata ggcgatggag gtctgcagcg acttggcgat
     6361 ttcatagagc ttgctggcgc tgacgcggtt ggcgccgcgc tcgtacttct ggacctgctg
     6421 aaaggtcagc cccagatcgt cggccagccg ttcctgtgag acgccgagca gtttgcgccg
     6481 catgcggatg cgaccgccga cgtgcaggtc cacgggattg ggggagcggt ctgtttcggt
     6541 tgtcgtcgtc atgagggcct gatctgtgga aatgcggatt tcacttcact gtccccctaa
     6601 ttcctattcc gctgcgattt caagctccag actacccgtt tgggtagata caccaggccc
     6661 gaaatgagca gcatcagcag aaatggaagg tctccaaagc gcctgtagac ggtttcgaac
     6721 cgctgccaag gtatatgccc gtcgataacg tcgctctccc ccaggcgcaa gacatggccc
     6781 gtcgcggcgc ctctggcgtc aatcatggcg gatacgcccg tgggggtcgc gcggaggatc
     6841 ggaatcccct cctcgatggc ccgatagctt gaaaggttga ggtgctggag cggcccgctg
     6901 gtgaccccga accaggcgtc gttggagacg ttgacgatga agtcaggcct tgcgccagaa
     6961 gccctagcgc cagtgcgggt atagccaggg tagagtcctt cgtagcagat caggggctgg
     7021 aaggcgggca ggccggggat ggtcatgggg cgcggcggcg gacccggcgt gaagccgtcg
     7081 ccgacgtgga ccagggtctt cacgcccagc ttttccatcc acgcgtcgag cggcagaaac
     7141 tcgccgaacg gcaccagccg gtacttgtcg tagacccccg cgggctcgaa tcccccggcc
     7201 gtgcggcgcg cggcgaccag gctgttgtag tagcgatacc gcccctgcgt cccttcgacg
     7261 cggtaggccc cgacgagcag catctgcccg ggcctgaggg cgccctcgat ggcggcccgg
     7321 gtccaggttc cgggggcgag atagttgtcg atcgcgtccg ggatcgcccc ttcgggccag
     7381 atgacgatgt ccgccacccg cccgtccggg tagggccgcg aggtcagggc gacgtaccgg
     7441 tcgacgatcc gctggaactc ggcgggatcg tacttggccg actgcttgat gtcggcctgg
     7501 accatccgga cggtcagggc gcgctggccg gacggcgccg gccgatccat cgagcgaaca
     7561 gcgccgaaca gccacaggcc cgcaagggcg gcggcggcca ggccgacggc gatccggccc
     7621 gcccgcccct cgcgccagac cgcgaccggg acgaccaggg ccagggtcac ccaggtcagg
     7681 ccataggcgc cgaccagcgc cgccgtctgc gacatcgccg atccggcctt ccaggtctcg
     7741 cccggcaggt tccagggaaa accggtcagc acatggccgc gcagccattc gcaggccgcc
     7801 agggctccga cgaacaccag cacccgggcc acgcccttcg cgcccgtcca gcgataggcc
     7861 aggcaggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnccccg tccgccgtgt cgaccagcca gaacagcagg ggatagccga gcagaccggg
     8101 cagaagcccg aacggagggt gggccaggcc ggtggccagg cccgcggcca gcgccagcgc
     8161 ccagcgcgga accctggcca ggtccagcgt cacgccgccg aggtctcctc ggccgtcgtc
     8221 ggcaacgggc gcggccggaa ccgcacgcgc ttcacccggc gcgggtcggc ggcgatcacc
     8281 tcgatatcgt aacggtccga caggggaacg acgtcgcccc gccggggcac ccgcccggcg
     8341 atggccgtga ccagaccgcc gaccgtgtcg atgtcctcct cgtgatcgcc atcgcccagc
     8401 tgatcctcgc cgagcaccgc ctcgaggtcc tcgagcaggg cgcgggcgct ggcctcgatg
     8461 acgccgtccg gcatcacccg caggccggtg acggccgcat cgtcgtgctc gtcggcgatg
     8521 tcgccgacca ccgcctcgat caggtcctcg agcgtcacca ggccctcggt gccgccgtat
     8581 tcgtcgatca ccagggcgag gtggacgcgg gtcttctgca tctgggccag caatgtcgcc
     8641 gcgcgcatcg agcccgggac ccccggcgcc gggcggcgca ggcgcttgag caccccggcc
     8701 gggcggcgac ccttgctggc cagcagcttg aacacgtcct tgatgtgcac gaagccgatc
     8761 gggtcatcga gcgtgccgcg atagaccggc agccggctgt gctcggtctc gctgaactgg
     8821 gcgacgagag cctcgaacgg tgtgttgaac tccaccgcca cgatgtcggc gcggggcgtc
     8881 atcacgtcgt cgacggtcag ggtctggaag gcgcgcgcct gggcggcgag ggtctcgccg
     8941 gcctgggtcg tcggctcgat ctcggccacc ggctcgccgg ccaggccgtg gcgaacgcgg
     9001 ccgaagagac tccagaatcc ccgccgcggg cgaggggttt cgggcgaact ggcgtcgtcg
     9061 gaatgggcca tctgtctcag gcgtagggat cggcaacgcc gaggccggcc aggatcagcc
     9121 gttccagcgc ttccatctcc tcggcttcgg tctccgtctg gtgatcatac cctacaagat
     9181 gcagcacgcc gtgcgccacc aggtgttgca ggtgatgggc cagggtcttg ccctgctgcg
     9241 ccgcctcccg ggcgcagaca ccgtaggcca gggcgatgtc gccaagatga ccctctggat
     9301 tggccggggc cgggaagctc agcacattgg tcggcccgga cttgccgcgg aagcgctcgt
     9361 tcaggtcggc gaccgtggcg tcgtcggtca gcaggaccgt caggccgccc tcggcggtcg
     9421 ccgcctcggc cgcctgcagc gccaggtgtt cggcggcggg cagcgcctcc acccaggcct
     9481 cgtcctcgat ctcgatatcg atgcccaaga tctactggcc cgtgcgcttg gcggcgtcgg
     9541 cgtcgtaggc gcggacgatg cgggcgacca tcggatggcg gatcacgtcg gcggcggtga
     9601 agcgggtcac cgaaacgccc tccacatcct ccaggatggc cacggcgtgg gccagaccag
     9661 agtcggcggg attgagcagg tcgatctgac tgggatcgcc ggtcaccgcc atgcgcgagc
     9721 cctcgcccag gcgggtcagg accatcttca tctgcagccg gctggtgttc tgcgcctcgt
     9781 cgacgatgac gaaggcatgg gccagggtcc ggccgcgcat gaaggcgatc ggcgccacct
     9841 cgatctcgcc cttgtcgcgg cggcggcgca gctggtcggc ccccataagc tgggtcagcg
     9901 cctcccagac cggggccatg taggggtcga ccttctcggt caggtcgccg ggcaggaagc
     9961 cgagccgctc gccggcctcg acggccggcc gggtgacgat cagccggtcg atgtcgccgc
    10021 gcatcagcat ggccgcgcca tgggccacgg ccaggaaggt cttgcccgtg ccggccgggc
    10081 cgaggccgaa caccaggtcg tggctgccga gggcttccat gtagcgggcc tgggtggcgg
    10141 tcttgggcga gaccgggccg cgacgcggcg cgccgggcgc cgccggcttg tg
//
LOCUS       DAORNX010000048        10141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4911_length_10141_cov_7.554531, whole genome shotgun sequence.
ACCESSION   DAORNX010000048 DAORNX010000000
VERSION     DAORNX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_10141_cov_7.554531"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="PLO65_03535"
     CDS             <1..125
                     /locus_tag="PLO65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRD27353.1"
                     /translation="ARLDMTALVDGYYAEVQAMGGNRWDTSSLAARLDLERAPS"
     gene            122..1267
                     /locus_tag="PLO65_03540"
     CDS             122..1267
                     /locus_tag="PLO65_03540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD27354.1"
                     /translation="MTLGVDFVVIGAGIAGVSAAWALAEHGRVLVLEAEAQAGYHATG
                     RSAALFHLIYGGPGVQPLSRASLEFFRAPPAGFTEVDLLRTRGILIVARSGQEADLGA
                     KVAEAPDALRVVTAGEARQLWPALRPGYVSAAAWDGSSADIDVDALHRGFIRGLTERG
                     STLRTGERVDQIERRRNGWRIRTPALSIECGAVVNAAGAWADENAVLAGVKTLGLRPK
                     RRTAALIDAPADARDWPMLIDAAESFYAKPSAGRMLLSPAEETDMEAHDAAADDLALA
                     EGVERLEAATTLTVHRKPLAWAGLRTFTPDRLPAVGEAPDRCGFFWLAGQGGFGVQTA
                     PALARLLASEVTGAPLPARWRDVGLSPDAYRPARLLSAQVSSHAPQS"
     gene            1296..2234
                     /locus_tag="PLO65_03545"
     CDS             1296..2234
                     /locus_tag="PLO65_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD27355.1"
                     /translation="MPRPDQFALVETRVNDAADGQVMVRNLYMSVDPVMRSQLNAMTP
                     LNEAMRGAAIGVVVQSRSPDFSVGDLVSHYLGFREYFLSDGEGLIRVRELPGLPITTH
                     LHALGMTGFTAYGGMLHIGAVKAGEQVFVSAAAGAVGSVAAQIARLKGCHVIGSVGSA
                     EKARWLKSVAGLDAVINYRDAPIAEALPAAASKGLDVYFDNVGGEHLDAALGCMNTLG
                     RIPVCGMISGYNDPSVAVRRLSTIVYRRVMLRGFSLMDFIDQREAFERDMTGWLRDGR
                     VRVEETVLDGIETAPTALIGLFQGVNLGKMLVRLGT"
     gene            2496..4547
                     /locus_tag="PLO65_03550"
     CDS             2496..4547
                     /locus_tag="PLO65_03550"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRD27356.1"
                     /translation="MDDNRLSRRAFAAQAAAIGAALAFGRTSAQAKPAALGERRDLFP
                     QGVASGDPRPDSVILWTRRTPDAGVAAHRLAVEVASDPEFRKLVARGDVAVTAATDWT
                     CRFLAAGLKPAREYWYRFIDEQGHTSRVGRTLTAPADDDDRPVRFAFVSCQDVTQGAL
                     NAYRRMLFEDARRPREEQLGFVLHLGDFIYELVWYPEERPGGMERGRRLRGNVRYPHG
                     EKVSTFHTPTDLDDYRTAYRGYLLDPDLQDARARWPFVPVWDNHEFNWRSYQSQQIIN
                     GEIRPAQKLKVAASQAWYEYQPAQVRKAGTGDVFEAPVVENRPVETFDARGVGQEPNN
                     LAAINALQIQRAFRYGRNVDMILTDNRSFISPGGSYSGLPDIKFPAMPDSMLEILDQG
                     RAYDGGHPPATIRLGELEFPNPNKDAPPEAYLGLEQMAWFKDRLRAARAPWKIWGHSF
                     GTLTGRTDPQNLPAAFADQWPAGAGYGVTTGAYRLEHREIFDMVRAEGITGLTIVAGD
                     RHAFWAGYPSSDLPPRAYDPVGVEFVTGSISAQTLGEVQQLTMPRDRPLRALSIHDRP
                     DGSMETSWNMTLLHGVKASLVLKETGDPAQARAARNPELSPHLRFLDYAGHGYATVRA
                     TPDELETEFVCIPIPLERSETDDGGPMRYRVTHRVALWQPGERPELRQAILEGDPGLA
                     I"
     gene            complement(4606..5694)
                     /locus_tag="PLO65_03555"
     CDS             complement(4606..5694)
                     /locus_tag="PLO65_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HRD27357.1"
                     /translation="MGERRTLRGLDDLADAGLLPAERLPALEAVAARYAVAVTPAMTA
                     LVDPADPGDPIGRQFIPDPAELDAQPMESADPVGDMAHSPVAGIVHRYPDRVLLTLTH
                     TCAVYCRFCFRREVVGPDGAGNLTPAQLDAALAYIAATPAIWEVILTGGDPLVLSPRR
                     LREVVDRLEAIGHVKVIRFHSRVPVVAPETVTGDLVAALKSRSRAVYVALHANHPREL
                     TPAARAACARIIDAGIPMLSQTVLLRGVNDDAATLAGLMRAFVETRIKPYYLHHGDLA
                     PGTGHFRVPIAEGQALMRALRGRVSGLCQPTYILDIPDGHGKVPIGPGYLTADADGPL
                     RIEDPSGVRHDYRGEASTRRTDQGGGRD"
     gene            complement(5694..6740)
                     /gene="epmA"
                     /locus_tag="PLO65_03560"
     CDS             complement(5694..6740)
                     /gene="epmA"
                     /locus_tag="PLO65_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRD27358.1"
                     /translation="MSTAFPTPWWRPDVHADRRPFLMARNRVKAGLRRWFEAQGFVET
                     EAACLQVSPGNEAHLHAFATEALDLAGGRQPLYLHTSPEFACKKLLAAGEAKIFEFAR
                     VWRNRERGPLHHPEFTLLEWYRAGAPYQVLMDDCASILALAAETAGTSRFRFRGMEVD
                     PFATPERITVAEAFDRFAGVDLLASLAADGTPDRAVLADAARAAAIRVADDDTWADIF
                     SRVMVEKVEPRLGAGRAVVLCEYPTAEAALARPAAHDPRVAERFELYACGVELANAFG
                     ELTDAVEQRRRFDAEMDEKARVYGERYPLDEDFLAALAVMPEASGAALGFDRLAMLAT
                     GAPRIEQVLWVPVV"
     gene            6909..7475
                     /gene="efp"
                     /locus_tag="PLO65_03565"
     CDS             6909..7475
                     /gene="efp"
                     /locus_tag="PLO65_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRD27359.1"
                     /translation="MKVAASSLRKGAVVELEDKLYVVLSADNIHPGKGTPVTQLNMRR
                     ISDGVKVAERYRTTEMVERAIVDQREHTFLYRDGEGFHFMNPETYEQYVGTEDVIGDA
                     AAYLQDGMSVSLSIHNQTPIAIELPRTATLEIVDTEPSMKGQTASSSYKPALLSNGVK
                     TMVPPYIAAGTRIVVLTEDGSYLERAKD"
     gene            complement(7500..8186)
                     /locus_tag="PLO65_03570"
     CDS             complement(7500..8186)
                     /locus_tag="PLO65_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRD27360.1"
                     /translation="MIEALFDRNLRWAAGRTRMDPEYFRRLAAQQSPDYLWIGCSDSR
                     VPANEIVGLDPGELFVHRNVANLAPSQDANFLSVLQFAVEILGVRHIIVCGHYGCGGV
                     RAAVSGQRHGLIDHWLQPVSCLCRQHADELDSILDVEARVNLVCERNVLAQVAGLADN
                     PFVRDAWGRGQALAVHGWIYSIQDGLLRDLDTSISGVSDIRARPRLPAPAPRGRRRGG
                     AATPRRPADR"
     gene            complement(8183..8734)
                     /locus_tag="PLO65_03575"
     CDS             complement(8183..8734)
                     /locus_tag="PLO65_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRD27361.1"
                     /translation="MTSPGSEIEPISPELMAEPLDFFFAEHFRHRQLCRLIDRLGATV
                     FFDADPIAEVVDFLRFEAPIHIIDEEEDLFPLLRRRCLPEDDLENVLGVLSAEHRADA
                     TLGRAVRTHLEHCLDTRSAPGASLEGRRDLGAFAAQERRHLALENAVVLPIARLRLTP
                     ADLTGLGRRLAARRGMMLAETGP"
     gene            complement(8754..9944)
                     /locus_tag="PLO65_03580"
     CDS             complement(8754..9944)
                     /locus_tag="PLO65_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27362.1"
                     /translation="MRRQSALLPLALAALAAPGLAHAAEGQGDWIVDARLRYEFVDQD
                     GFSEEAQALTLRTRLGYETPAWNGLKALVEAENVTALSDSYNSTTNGQIAYPTVLDPE
                     ATEINRAQVSWTGARASAVVGRQRIILGNARFVGNVGFRQNEQTFDAARVSFKVDPTT
                     TLTWIYIDRVQRILGDDSPQGEWDSDSHVIQLETKTPLGQLTAYGYLLDFDTAPLQSS
                     ATWGARLTGSRPLSGGLAVTWEAEYARQTDYGSNPQDFDLDYLALAAGLKKDTRFVSI
                     GLERLDGNGVRGFSTPLATLHAFQGWADVFLATPARGVTDLNLTAGTALPVGARKLKL
                     VAAAHDFSEADGGLDYGREFDASASVPLTPTLSLEAKAARFVGDRPAFADRTKIWLTL
                     ELKL"
     gene            complement(9950..>10141)
                     /locus_tag="PLO65_03585"
     CDS             complement(9950..>10141)
                     /locus_tag="PLO65_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD27363.1"
                     /translation="DAEPAAWVRTVVADFVKGGDDEAWTRVISAAQGGGDPALAALGA
                     ILKSRLAPAKATFTLIRRR"
BASE COUNT         1569 a         3521 c         3546 g         1505 t
ORIGIN      
        1 gcgcgcgact ggacatgacc gccctggtcg acggctacta cgccgaggtc caggccatgg
       61 gcggaaaccg ctgggacacc tccagcctgg ccgcccggct ggacctggaa cgggcgccat
      121 cttgaccctc ggcgtcgact tcgtcgtcat cggcgccggc atcgccggag tgtcggcggc
      181 ctgggccctc gccgagcatg gcagggtgct ggtgctcgag gccgaggcgc aggcgggcta
      241 ccatgcgacc ggccgttcgg cggccctgtt tcacctgatc tacggcggtc cgggtgttca
      301 gcccctcagc cgcgcatccc tcgagttctt cagggcgccc ccggcgggct tcacggaagt
      361 cgatcttctg aggacccgag gaatcctgat tgtcgcccgg tccgggcagg aggctgacct
      421 tggggcaaag gtcgccgagg cgccggacgc gctgagggtc gtgaccgcgg gcgaggcgcg
      481 acagctgtgg cccgccctgc ggcccggcta cgtatcggcg gccgcatggg atggatcctc
      541 cgccgacatt gacgtcgacg ccctgcaccg gggtttcatt cgcgggctga ccgagcgcgg
      601 atcgaccctc cgaaccggcg agcgggtcga tcagatcgaa cgccggcgga acggttggcg
      661 tatcaggaca cccgcgcttt ccatcgagtg cggcgccgtg gtgaacgccg cgggcgcctg
      721 ggccgacgag aatgcggtcc tggctggcgt gaagacgctg ggcctgcgcc cgaaacgccg
      781 cacggccgcc ttgatcgatg cgccggcgga cgcgcgagac tggcccatgt tgatcgacgc
      841 cgccgagtcc ttctacgcca agccgtcggc gggtcggatg ctgctctcgc cggcagagga
      901 gaccgacatg gaagcccacg acgcggcggc cgacgacctg gcgctggccg agggcgtcga
      961 gcggctggaa gccgcgacaa ccctgacggt tcaccgcaag cccctggcct gggcgggatt
     1021 gcggactttc acccccgacc ggcttccggc cgtcggcgag gcgcccgacc gttgcggctt
     1081 tttctggctc gccggccagg gcggcttcgg cgtccagacc gcgccggccc tggcgcgtct
     1141 tctcgcctcg gaggtcaccg gagcgccgct tcccgccagg tggcgggatg ttggcctgtc
     1201 ccccgatgcc tatcgcccgg cccgacttct ttccgcacag gtttccagcc atgcccctca
     1261 gtcgtgaagt tcatctgatc gcgcgccccg ccggcttgcc cagacccgac cagttcgccc
     1321 tggtcgagac ccgggtgaat gacgccgcgg acggccaggt gatggtccgg aacctctaca
     1381 tgtccgtgga cccggtcatg aggtcacagc tcaatgccat gacccccttg aacgaggcca
     1441 tgcgcggagc cgccatcggt gtcgtcgtcc agtcgcggtc accggacttc tcggtcggcg
     1501 atctggtctc tcactatctt ggcttccgag agtacttcct gtccgacggc gaggggctga
     1561 tccgggtcag ggagcttcct ggacttccga tcacgaccca cctccatgcg ctcggcatga
     1621 ccggcttcac cgcctacggc ggcatgctgc atatcggggc ggtcaaggct ggcgaacagg
     1681 tcttcgtgtc cgccgccgcc ggcgctgtcg gatcggtcgc cgcccagatc gccaggctca
     1741 aggggtgcca cgtcatcggg tccgtggggt ctgccgagaa ggcccgctgg ctgaagtccg
     1801 tcgccggcct ggacgccgtc atcaactatc gcgacgcgcc aatcgcggag gctcttcccg
     1861 cggccgcgtc caaggggctc gatgtctatt ttgacaatgt gggcggcgag cacctcgacg
     1921 ccgccctcgg gtgcatgaac accctgggcc gaattccggt ttgcggaatg atttccggct
     1981 acaacgatcc gtccgtcgcg gttcgaaggc tttcgacgat cgtttatcgg cgcgtcatgc
     2041 tccgggggtt cagcctgatg gacttcatcg accagcgcga ggccttcgag cgcgacatga
     2101 ctggctggtt gcgagacgga cgcgtccgtg tcgaggaaac cgtccttgat ggcatcgaga
     2161 ccgcccccac cgccctgatc gggctgtttc agggcgtcaa cctgggcaag atgctggtca
     2221 ggctcgggac ctagaaccgg tgtcgcgccg cgccgccggg cgcgcccggg tcacagatcc
     2281 tgccatgtcg gcctgcccgc cgcgccaacc gttccatgcg tgatccggac cctgatcgtt
     2341 ccccggatga cggccctcac cccggccgcc tgaacgaaag ttaacttgcg acctccggac
     2401 gaaagtcttc cgtgaaccgt cagatcccgc cgcgggccgg cgggatcgat ggggcgaccg
     2461 ctcgacggga cgcgtcactt cgggaggggt ggaccatgga cgacaacagg ctctcgcggc
     2521 gggccttcgc cgcccaggcc gcggcgatcg gcgcggcgct ggcgttcgga cggacctccg
     2581 cccaggcaaa gcctgccgcg ctgggcgaac ggcgggacct tttcccacag ggcgtcgcct
     2641 ccggcgatcc ccgtccggac agcgtgatcc tctggacgcg gcggacccct gacgccggcg
     2701 tcgccgcgca ccggctggcg gtcgaggtgg ccagcgatcc ggaattcagg aagctcgtcg
     2761 cgcgcggcga tgtcgcggtc accgcggcga ccgactggac ctgccgcttc ctggccgcgg
     2821 gactgaagcc ggcgcgggaa tactggtacc gcttcatcga cgagcaggga cacacgagcc
     2881 gggtcggccg caccctgacg gcgccggcgg acgacgacga tcgcccggtc aggttcgcct
     2941 tcgtcagctg ccaggacgtc acccagggcg ccctcaacgc ctaccggcgc atgctgttcg
     3001 aggacgcgcg ccggccgcgc gaggaacagc tgggcttcgt cctgcacctg ggcgacttca
     3061 tctacgaact ggtctggtat cccgaggaac ggccaggcgg catggagcgg ggccggcgcc
     3121 tgcgcggcaa tgtgcggtat ccgcacggcg agaaggtcag caccttccac accccgaccg
     3181 atctggacga ctaccgcacg gcctatcgcg gctacctgct cgatccggac ctgcaggacg
     3241 cccgggcccg gtggccgttc gtccccgtct gggacaacca cgagttcaac tggcggtcct
     3301 accagagcca gcagatcatc aacggcgaga tccgcccggc ccagaagctc aaggtcgcgg
     3361 cgagccaggc ctggtacgag taccagcccg cccaggtccg caaggccggg accggcgatg
     3421 tcttcgaggc gcccgtcgtc gagaacaggc ccgtcgagac cttcgatgcg cggggcgtcg
     3481 ggcaggagcc caacaacctg gcggccatca acgcgctgca gatccagcgg gcgttccgat
     3541 acggccggaa cgtcgacatg atcctgaccg acaatcggtc cttcatctcg ccgggcggca
     3601 gctacagcgg gctgccggac atcaagttcc cggccatgcc cgactcgatg ctcgagattc
     3661 tcgaccaggg ccgggcctat gacggcggcc atccgccggc caccatccgc ctcggtgagc
     3721 tggagtttcc caatccgaac aaggatgcgc cgccggaggc ctatctcggc ctcgagcaga
     3781 tggcctggtt caaggaccgt ctgcgcgccg ccagggcgcc gtggaagatc tggggccatt
     3841 cgttcggcac cctcaccgga cgcaccgatc cgcagaacct gccggcggcg ttcgccgatc
     3901 agtggccggc gggtgcgggt tacggcgtga ccaccggcgc ctatcgcctg gagcaccgcg
     3961 agatcttcga catggtccgc gccgaaggaa tcaccggact gaccatcgtg gcgggggaca
     4021 ggcacgcgtt ctgggccggc tatccctcca gcgacctgcc gcctcgcgcg tacgatccgg
     4081 tcggcgtcga attcgtcacc ggctcgattt cggcccagac cctgggtgag gtgcagcaac
     4141 tcaccatgcc cagggatcgc cccctgcgcg ccctcagcat ccatgaccgg cccgacggct
     4201 cgatggagac cagctggaac atgaccctgc tgcacggcgt gaaggcctcc ctggtcctga
     4261 aggagacggg cgaccccgcc caggcgcgcg cggcgcgcaa tccggaactg tcgccgcacc
     4321 tcaggttcct ggactacgcc ggtcatggct atgcgaccgt tcgggcgacg cccgacgagc
     4381 tcgagaccga attcgtctgc atccccatac cgctggagcg cagcgaaacg gacgatggcg
     4441 gcccgatgcg ctatcgcgtg acgcaccgcg ttgccctctg gcagcccggc gagcggcccg
     4501 agctccggca ggccatcctc gaaggtgatc cgggcctcgc catctgaagg gtgtgcagct
     4561 gaccttgcct cgcccggcgt ctgacatcgc gacgggggac cccggtcagt cgcggccgcc
     4621 gccctggtcg gtcctgcggg tcgaagcctc gccgcgatag tcgtggcgga cgcccgaggg
     4681 gtcctcgatc cgtaggggcc cgtcggcgtc ggcggtcagg tagccagggc cgatcggcac
     4741 cttgccgtgc ccgtccggga tgtcgaggat gtaggtcggc tggcagagac cggagacccg
     4801 gccacgcagg gcccgcatca gcgcctgacc ctcggcgatc ggcacccgga aatggccggt
     4861 gcccggcgcg agatcgccgt ggtgcaggta gtagggcttg atccgcgtct cgacgaaggc
     4921 ccgcatcagc ccggcgagcg tcgcggcgtc atcattgacc ccgcgcagca gcaccgtctg
     4981 gctgagcatc ggaatcccgg cgtcgatgat cctggcgcag gcggcccggg ccgcgggcgt
     5041 cagctcacgc gggtgattgg cgtgcagggc gacatagacg gcccggctcc ggctcttcag
     5101 cgccgcgacc aggtcgccgg tgactgtctc gggcgccacc acgggcacgc gactgtggaa
     5161 acggatcacc ttcacatggc cgatggcctc cagccgatcg acgacctcgc gcagccggcg
     5221 gggcgacagg accagcggat cgccgccggt caggatcacc tcccagatgg ccggggtcgc
     5281 cgcgatatag gccagggccg cgtcaagctg cgccggggtc aggttccccg ccccgtcggg
     5341 cccgaccacc tcgcgccgga agcagaagcg gcagtagacg gcgcaggtgt gggtcagcgt
     5401 cagcaagacc cggtcgggat agcgatgaac gatccccgcc accgggctgt gcgccatgtc
     5461 gcccaccgga tcggccgact ccatcggctg ggcgtcgagt tcggcgggat ccgggatgaa
     5521 ctggcggccg atcgggtcgc cggggtcggc cgggtcgacc agggcggtca tcgccggagt
     5581 gacggccacg gcatagcgcg ccgccacggc ctcgagcgcc ggcaggcgtt cggctggcag
     5641 caggccggcg tcggccaggt cgtcgagacc gcgcagggtg cgacgttcgc ccatcagacc
     5701 accggaaccc agaggacctg ctcgatgcgc ggggcgccgg tcgcgagcat ggccagccga
     5761 tcgaagccca gcgccgcgcc ggaagcctcg ggcatgaccg ccagcgccgc caggaaatcc
     5821 tcgtcgagcg ggtagcgttc accgtagacc ctggccttct cgtccatctc ggcgtcgaag
     5881 cggcggcgct gttcgaccgc gtctgtcagc tcgccgaagg cgttggccag ctcgacgcca
     5941 caggcgtaga gctcgaaccg ttcggccacg cgcggatcgt gcgccgcagg ccgggcaagg
     6001 gccgcctcgg cggtgggata ctcgcagaga acgacggccc ggccggcgcc aagcctgggc
     6061 tcgactttct cgaccatcac ccggctgaag atgtcggccc aggtgtcatc gtcggcgacg
     6121 cggatggcgg cggcgcgggc ggcgtccgca agcaccgcgc ggtccggggt cccgtccgcc
     6181 gcgagactgg ccaacagatc gacgccggcg aaccggtcga aggcctcggc cacggtgatc
     6241 cgttccggtg tcgcgaaggg atcgacctcc atcccgcgga agcggaagcg cgaggttccg
     6301 gcggtctccg ccgccagggc gaggatcgac gcgcagtcgt ccatcaggac ctggtagggc
     6361 gcgccagccc ggtaccattc cagcaaggtg aattccggat ggtgcagcgg cccgcgctcg
     6421 cggttgcgcc agacccgggc gaactcgaag attttcgcct cccccgcggc cagcagtttc
     6481 ttgcaggcga actccggcga ggtgtgcaga tagagcggct gccggcctcc tgccaggtcg
     6541 agcgcctcgg tggcgaaggc gtggaggtgg gcttcattgc cgggcgagac ctgcaggcag
     6601 gcggcctcgg tctcgacgaa gccctgggcc tcgaaccagc gccgcagacc cgccttgacg
     6661 cggttgcgcg ccatcaggaa cggccgccgg tcggcgtgga catcgggtct ccaccagggg
     6721 gtcggaaagg cggtgctcaa ggtcggcgat accgggtctg acgatgtcga ccggacaggc
     6781 gggcgacggg aacaagcgag aattgctggc gattctggcc cgggacgcta tggagcgggg
     6841 gaaccaattc ttccagcaca attgtgagcg gcccgacggg cggccacaga acaccaggga
     6901 cgtaccacgt gaaggttgcc gccagttcgc tccgcaaggg cgccgtcgtc gaactcgaag
     6961 acaagctcta tgtcgttctg agcgccgata acatccaccc cggcaagggc acgccggtga
     7021 cccagctcaa catgcgccgc atctccgacg gcgtgaaggt cgccgaacgc taccggacca
     7081 ccgagatggt cgagcgcgcc atcgtcgacc agcgcgagca caccttcctg tacagggacg
     7141 gcgaagggtt ccacttcatg aaccccgaga cctacgagca gtatgtcgga accgaggatg
     7201 tgatcggcga cgccgccgcc tacctgcagg acggcatgtc cgtgtcgctg tcgatccata
     7261 accagacgcc gatcgccatc gaactgcccc gcaccgcgac gctggaaatc gtcgataccg
     7321 aaccgtcgat gaagggccag accgcctcct cgtcctacaa gcccgcgctg ttgtccaacg
     7381 gggtcaagac gatggtcccg ccctacatcg ccgccggcac gcgcatcgtc gtcctgaccg
     7441 aggacgggtc ctatctcgag cgcgccaagg actgacgccg ccagccgccg ggtcggccgt
     7501 cagcgatcgg ccgggcgacg gggcgtggcc gccccgccgc gccttcgacc gcgtggcgcg
     7561 ggggccggaa gccggggacg ggcgcggatg tcggagacgc cgctgatcga ggtgtcgagg
     7621 tcccgtagca ggccgtcctg gatcgagtag atccagccat gcaccgccag cgcctggccg
     7681 cgaccccagg cgtcccggac gaacggattg tcagcgaggc cggcgacctg ggccagcacg
     7741 ttgcgttcgc agacgagatt cacccgcgcc tcgacgtcga ggatggagtc cagttcgtcg
     7801 gcatgctggc ggcacaggca ggacacgggc tggagccagt ggtcgatcag cccgtgccgc
     7861 tggcccgaga ccgccgcgcg gacgccgccg cagccatagt ggccgcagac gatgatgtgg
     7921 cgcacgccca gaatctccac cgcgaactgc agcacggaca gaaagtttgc gtcctgcgac
     7981 ggcgcgagat tggcgacgtt gcggtgcacg aacagttctc ccggatcgag gccgacgatc
     8041 tcgttggccg gcacccggct gtccgaacag ccgatccaca ggtagtccgg cgactgctgg
     8101 gcggccagcc ggcggaagta ttccgggtcc atccgcgtcc ggccggcggc ccagcgcagg
     8161 ttgcggtcga acagggcctc gatcacggcc cggtctccgc gagcatcatg ccgcggcgcg
     8221 ccgccagccg ccggcccaga cccgtcaggt ccgcgggtgt caggcgcagc cgggcgatcg
     8281 gcagcaccac ggcgttctcg agcgcgaggt gccggcgctc ctgggccgcg aaggccccca
     8341 ggtcacgcct tccctccaga ctggcgccgg gggcggaccg ggtatcgagg caatgctcca
     8401 ggtgggtcct gaccgcccgc ccgagcgtgg cgtcggcccg gtgctcggcc gacagaacgc
     8461 ccaggacgtt ctcgagatcg tcttccggca ggcaccggcg acggagcagg ggaaacagat
     8521 cctcctcctc gtcgatgatg tggatcggcg cttcgaacct gaggaagtcg acaacctccg
     8581 cgatcgggtc tgcatcgaag aagaccgtcg cacccagccg gtcgatcagc cggcagagct
     8641 gacggtggcg gaaatgctcg gcgaagaaga agtcgagtgg ctcggccatc aactcgggcg
     8701 agatcggctc gatttcgctg cccggggagg tcatggccgg cgcccttcca tcgtcagagc
     8761 ttcagttcga gcgtgagcca gatcttggtg cggtcggcga aggccggccg gtcgcccacg
     8821 aaccgcgccg ccttggcttc cagcgacaat gtcggcgtca gcggcacgga ggccgaggcg
     8881 tcgaactccc gtccgtagtc gaggcccccg tcggcctcgg agaagtcgtg tgcggccgcc
     8941 accagcttca gcttgcgggc cccgaccggc agcgccgttc cggccgtcag gttgaggtcc
     9001 gtgacgcccc tggccggcgt ggccaggaat acgtccgccc agccctggaa ggcgtgcagt
     9061 gtggccagcg gcgtcgagaa gccgcgcacg ccgttgccgt cgaggcgctc cagcccgatc
     9121 gagacgaacc gcgtgtcctt cttcagccct gcggccaggg cgaggtagtc gagatcgaag
     9181 tcctgcgggt tggagccgta gtcggtctgg cgcgcgtatt ccgcctccca ggtcaccgcc
     9241 aggccgccgg aaagcggccg cgacccggtc agacgggcgc cccaggtggc gctggactgc
     9301 agcggcgcgg tgtcgaagtc gagcagatag ccataggccg tcagctggcc cagcggcgtc
     9361 ttcgtctcga gctgaatcac atgggagtca ctgtcccact cgccctgggg gctgtcgtcg
     9421 ccgaggatac gctggacccg gtcgatgtag atccaggtca gggtggtggt cgggtcgacc
     9481 ttgaacgaga cccgggccgc gtcgaaggtc tgctcgttct ggcggaagcc gacgttgccg
     9541 acgaaccggg cgttgccgag gataatccgc tgccggccga ccaccgcgct ggcccgcgcg
     9601 ccggtccagg agacctgggc gcggttgatc tcggtcgcct ccgggtcgag cacggtcgga
     9661 taggcgatct gcccgttggt ggtgctgttg tagctgtccg acagggccgt gacgttttcc
     9721 gcctcgacca gcgccttgag gccgttccag gccggcgttt cgtagcccag ccgggtccgc
     9781 agggtgaggg cctgggcctc ctcgctgaat ccgtcctggt cgacgaactc gtagcgcagc
     9841 cgggcgtcga cgatccagtc gccctgccct tccgcggcat gggcgaggcc gggagcggca
     9901 agggcggcga gcgccagggg cagcagggcg gattgacggc gcatggcggt cagcgtctcc
     9961 ggatcagagt gaaggtggcc ttggcgggcg cgagccgcga cttgaggatg gcgcccagcg
    10021 ccgcgagggc cgggtcgccg ccgccctggg cggcggagat cacgcgggtc caggcctcgt
    10081 cgtcgccgcc cttgacgaaa tcagcgacaa ccgtccgcac ccaggcggcc ggttcggcgt
    10141 c
//
LOCUS       DAORNX010000049        10138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4916_length_10138_cov_8.964396, whole genome shotgun sequence.
ACCESSION   DAORNX010000049 DAORNX010000000
VERSION     DAORNX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_10138_cov_8.964396"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLO65_03590"
     CDS             <1..318
                     /locus_tag="PLO65_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRD27364.1"
                     /translation="ATPPRITPRAAPGALVTLERPDQGPNLIFPPDGATVQVDAFGPS
                     SRGLVLAAGGEGLNWYVEGRPLPPDPVSGRTLWRPAAAGFYELTVVDGQGRSARAKVR
                     IKG"
     gene            complement(355..972)
                     /locus_tag="PLO65_03595"
     CDS             complement(355..972)
                     /locus_tag="PLO65_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRD27365.1"
                     /translation="MLDDLKENNRRWSRRKTEVDPNFFRRLENQQSPEYLWIGCSDSR
                     VPANEIVDLDPGELFVHRNVANLAPPQDANYLSVLQFAVDVIKVKHVMVVGHYGCGGV
                     RAAVDGERRGLVDHWLHPIREVYAANREELEALDDKDRWDRLCELNVIRQVQNVASDV
                     FVQDAWARGQTLSVHGWCYRLSNGLINDLGVTVSNPGEAEELKQG"
     gene            complement(983..1621)
                     /gene="maiA"
                     /locus_tag="PLO65_03600"
     CDS             complement(983..1621)
                     /gene="maiA"
                     /locus_tag="PLO65_03600"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRD27366.1"
                     /translation="MGLLLHSAWRASAPYRVRIGLNLKGLAYDYAPVDLVVGQQRGEA
                     YGAVNAQHLVPALEVDGRILTQSLSILEWLEETHPEPPLLPKDPFDRQVVRAMCGVVA
                     CDIHPVNNLRILKALAKLEVGQPAIDDWVHRWIGEGFAALEPMIARHGAGFAFGATPT
                     MADCLLVPQVYNARRFNTDLAPFPALVAAADAAARHPAIAAAHPNNQPDAQV"
     gene            complement(1621..2271)
                     /locus_tag="PLO65_03605"
     CDS             complement(1621..2271)
                     /locus_tag="PLO65_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD27367.1"
                     /translation="MTQFAVPIRPQPVVPVAGSDRPFPVRRILCVGRNYAAHRREMGG
                     DDRDPPFFFSKPADAVVPPGADVPYPSATRDLHHEIELVVALGEGGTIFGFAVGVDLT
                     RRDLQNGFKARGQPWDAAKAFDASAPVSAIVRATGVIPQGRITLSVNGETRQDGQVAD
                     MIWSVEEILVEAGKLWKLEAGDLVFTGTPEGVGPLVRGDRVEGSVEGVGSLSFGIV"
     gene            complement(2563..4380)
                     /locus_tag="PLO65_03610"
     CDS             complement(2563..4380)
                     /locus_tag="PLO65_03610"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRD27368.1"
                     /translation="MSAPVLSIDDLRVSFQTHDGLVEAVRGVSLSVAAGECLGVVGES
                     GSGKSQTFMTVMGLLAGNGRATGSARFEGQELLGLRPADLNRIRGSKMTMIFQDPLTA
                     LTPHVKIGEQIAEPLRLHLGLSGADAARRAREWLDRVRIPEAARRMDQYPHELSGGMR
                     QRVMIAGAMACGPRLLIADEPTTALDVTVQAEILDLMAELMRETGSGMVLVTHDMGVI
                     ARLADRVCVMKDGAYVEEGPVETVFDQPRTDYTRGLLAAIPRLDRPDRGGRPTPAPVA
                     AAAPVVVRGEDVKVWFPVSQGLFGKPRPLRAVDGVSFEIRQGETLGVVGESGCGKSTL
                     SRAALNLLPPGGGELGGSVTVLGRDITHADRKAMRAARKDLQIVFQDPLASLDPRMPI
                     GDSIAEPLQVFRPGLGRQARREAAAAMMERCGLAPDLVNRYPHELSGGQNQRVGIARA
                     MILEPKLVICDEAVSALDVSIRAQIIDLLIDLQRQLGMAMMFISHDLAVVREVSHRVM
                     VLYLGRVMEQASREQIYGDARHPYTRALLSAAPIPDPKIERTRERVRLSGEPPSPMDP
                     LAALRFLPSRLPARPTDPVYRPALEAVAPGHLVSEFDPV"
     gene            complement(4466..4693)
                     /locus_tag="PLO65_03615"
     CDS             complement(4466..4693)
                     /locus_tag="PLO65_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27369.1"
                     /translation="MVTLEFRLPEGPEADQFDAAVYRFIERADATGMADAVALMQSTP
                     LLDHVHKAVTFARPSCAHDFNRFWASFPRRA"
     gene            4980..5867
                     /locus_tag="PLO65_03620"
     CDS             4980..5867
                     /locus_tag="PLO65_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HRD27370.1"
                     /translation="MWRATAPALGGKGPLAATPPRIIVVANEKGGAGKSTVAALLSTA
                     MLYQGRRVAVVDVDLRQQTLSHFFANRRRWLPAANVSAPVPLEYMLGDDPAELSEADP
                     AKVVTLFHRALTLAMAAADIVVIDTPGGDTPLSRAAHLQADIVVTPMNDSFVDFDLLG
                     KIDPITLKLVRPSLYAKVVHDARRARADHGRALDWVVLRNRMAATDGRNRQRVTVNLE
                     QLAGQVGFRLGPPLRERIAYREMFPFGLTLADLSANLRPAEISTPRRAAREELKALLV
                     ALNLIDEVQPDAAVLNSAG"
     gene            5975..7006
                     /locus_tag="PLO65_03625"
     CDS             5975..7006
                     /locus_tag="PLO65_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRD27371.1"
                     /translation="MFASLGRLGGDSAAPQRYPFRLKRRPEERVTLLGQAMDLVKAEE
                     VLFFVANHVAQKKRAIVANHNAHSLYLLRDDAELRAFYGRADLVEVDSRPLLMWARLT
                     GRSSRAFHRCTYLDWRDEFWALAAARGWRVYYLGAAPGVAEKAAELLRLGRPGVRIGV
                     HDGYFDMSPGSPEAGAVLDDIRAFSPDVLMVGMGMPRQEVWIHRNYDSLPPCVVLPVG
                     AAFDYEAGVQRAAPRWLGQIGLEWLFRLVVDPRRLFRRYCVEPWRLVGLLVDDAVNSA
                     RQRRATALRERRRAIAPPQDAPRRRASDQRGRVNLDPSIGGVPELQTGAGARRPAADR
                     RVATLSSEG"
     gene            7089..9158
                     /locus_tag="PLO65_03630"
     CDS             7089..9158
                     /locus_tag="PLO65_03630"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRD27372.1"
                     /translation="MSQTPAAAPPFDSPYRHGFVRLAACVPRITLADPAANARETLAL
                     LRRGHDRNVALMVFPELGLSAYSIDDLLQQDVLLDAVEAAIGQLVESSRSLFPVFAVG
                     APLRFDGRLFNTAVVIHRGEILGVIPKTFLPNYREYYERRWFASGLGVTGQTIAVAGR
                     AAPFGTDLLFRSTGSAPFTFHAEICEDVWTPEPPSTLAALAGAEILLNLSASNIVIGK
                     AEARRLLCASQSMRCQAVYAYSAAGVGESTTDNAWDGQASIFELGELLVESERFPAES
                     TLAMVDVDLGRVRQERFRTGSFGDSVARRQSNTPFRVQTFRLDAPTTTLALARAVPRF
                     PFVPDDEGRLAEQCHEAYSVQVQGLAQRLRSTGLERLVIGVSGGLDSTQALLVACKVM
                     DQLGLPRANILAFTMPGFGTSERTRANAWTLMNTLGVTAAELDIRPAATQMLGDLGHP
                     FAEGEPVHDVTFENVQAGLRTDYLFRIANQRGGLVVGTGDLSELALGWCTYGVGDHMS
                     HYNPNGSVPKTLIQHLIRFVAAAGETGPAGAAVLGDILATEISPELVPAGADGRGQAT
                     EDVVGPYALQDFNLFHLTRYGMAPSKIAYLAIAAWSDVSRGQWPPGLPAGDRRAYGAE
                     EITAWLKVFLRRFFANQFKRSAVPNGPKISSGGALSPRGDWRAPSDAGPRAWLEELGA
                     NGPAGNG"
     gene            9444..>10138
                     /locus_tag="PLO65_03635"
     CDS             9444..>10138
                     /locus_tag="PLO65_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD27373.1"
                     /translation="MKKKALLLGTMLGALALAPQAFAQSTGLYGAFDLGYHQPDDLDV
                     HSAVFGTSANVGLEGDIVGFARLGYRIMPNWRVELETGLRPGDIDEVPTDINGHFQAL
                     SIMGNVLYDFAPDASINPFFGVGAGVVQGHLAAVTPSVWLVDSDDRDSAFAYQVLAGL
                     SAPLSDQLSMDFTYRYIKATDIKIENPALAGPLEIGYEDQSVTFGLRYSLQQEAAPPP
                     PPPPPPPPPPPPPP"
BASE COUNT         1540 a         3468 c         3486 g         1644 t
ORIGIN      
        1 gccacgccgc cgcggatcac gccgcgcgcc gcgccgggcg cgctggtcac gcttgagcgg
       61 cccgaccagg gcccgaacct gatcttcccg cccgatggcg caacggttca ggtcgacgcc
      121 ttcggaccgt catcgcgcgg gctggtgctg gccgcgggcg gggaggggct gaactggtac
      181 gtcgagggcc ggccgctgcc gcccgaccct gtcagtggac ggacgctctg gcggcccgcc
      241 gcggccggct tctacgaact gaccgtcgtc gacggccagg gccgctccgc gagagccaag
      301 gtgcgcatca aggggtaggg cgcccgccgg ggcaggcctt ccggcgcccc ggcgtcagcc
      361 ctgcttcagc tcctcggcct cgccgggatt cgacaccgtc acgcccagat cgttgatcag
      421 gccgttcgac agccggtagc accagccgtg cacggagaga gtctgcccac gggcccaggc
      481 gtcctgcacg aacacgtccg acgcgacgtt ctgcacctgg cggatgacgt tgagctcgca
      541 caggcgatcc caacggtcct tgtcgtcgag cgcctcgagc tcctcgcggt tggcggcgta
      601 gacctcgcgg atcgggtgca gccagtggtc gacgagaccc cggcgctcgc cgtcgacggc
      661 cgcccgcacg ccgccgcagc cgtagtggcc gacgaccatc acatgcttga ccttgatcac
      721 gtcgaccgcg aactgcagca cgctgaggta gttggcgtcc tgcggcgggg ccaggttggc
      781 cacgttgcgg tggacgaaca gctcgcccgg gtcgagatcg acgatctcgt tggccggaac
      841 gcggctgtcg gagcagccga tccacaggta ttccgggctc tgctggttct cgaggcggcg
      901 gaaaaagttc ggatcgacct cggtcttgcg gcgcgaccag cggcggttgt tttccttcag
      961 atcatcaagc acggcggctc ccctacacct gggcgtcagg ctggttgttc ggatgggcgg
     1021 cggcgatggc cgggtgccgc gcggccgcgt cggcggcggc caccagggcc gggaagggcg
     1081 ccaggtccgt attgaaccgg cgggcgttgt agacctgagg caccagcagg cagtcagcca
     1141 tcgtcggcgt ggcgccgaag gcgaacccgg cgccgtgtct ggcgatcatc ggctcgagcg
     1201 cggcgaatcc ctcgccgatc cagcgatgca cccagtcatc gatcgccggc tggccgacct
     1261 cgagcttcgc cagcgccttg aggatgcgca ggttgttcac cgggtggatg tcgcaggcga
     1321 ccaccccgca catggcccgc acgacctggc ggtcgaacgg atccttcggc agcagcggcg
     1381 gctccggatg ggtctcctcc agccattcaa ggatcgacag cgactgggtc aggatccgtc
     1441 cgtcgacctc gagcgccggc acgaggtgct gcgcgttgac cgcgccgtag gcctcgcccc
     1501 gctgctgtcc gacgacgagg tcgaccgggg cgtagtcata ggccaggccc ttcaggttca
     1561 gcccgatccg cacccggtag ggcgccgagg cgcgccaggc gctgtgcagc agcaatccca
     1621 tcagacgatc ccgaacgaca gcgatccgac gccctcgacc gatccctcga cccggtcgcc
     1681 acggaccagg gggccgacgc cttcgggcgt gccagtgaaa actaggtcgc cggcttcgag
     1741 tttccagagc ttgccggcct cgacgaggat ttcctcgacc gaccagatca tgtcggcgac
     1801 ctggccgtcc tggcgggtct cgccgttgac gctcagggtg atgcggccct gcgggatgac
     1861 gccggtggcg cggacgatgg ccgagaccgg cgcgctggcg tcgaaggcct tggccgcgtc
     1921 ccacggctgg cccctggcct tgaaaccgtt ctgcaggtcg cgccgggtca gatcgacgcc
     1981 gacggcgaag ccgaagatcg tcccgccctc gccgagcgcc acgaccagtt cgatctcgtg
     2041 atgcaggtca cgcgtggccg agggataggg gacatcggcg ccgggcggga ccaccgcgtc
     2101 ggccggcttg ctgaagaaga acggcgggtc gcgatcatcg ccccccatct cgcgacggtg
     2161 ggcggcgtag ttgcggccga cacagaggat acggcgcacc ggaaagggtc gatcggaccc
     2221 cgcgacaggc acgaccggct ggggacggat cggaacggca aactgggtca tggcggcttc
     2281 ctagccaaag cggccgtcaa tgtctcgcag ccactgaggc gtaaggcatc acttgacccg
     2341 tattagttga gataccgacg ttcgatgaag tccttgcggc tctcgctgtc atgcttatgc
     2401 tggcgggaaa cattggggga gtaagaggtt gctcaacctc ctgaagctgc tcaagtgcct
     2461 gcgcgtcggg catcggtgga agaacagccg tcagaagtcc ggcttccaga cgtgcgaacg
     2521 ctgcggcctc cgcgtgaagt actagcgacc tccgacgccg ggtcagaccg ggtcgaactc
     2581 gctgaccaga tgccctggag cgaccgcctc cagggccgga cgatagaccg ggtcggtggg
     2641 ccgggccggc aaccgcgagg gcaggaagcg tagcgccgcc agcggatcca tcggcgacgg
     2701 cggttcgccc gacagccgca cccgctcgcg ggttctttct attttcgggt cggggatcgg
     2761 cgccgccgac agcaacgccc gggtgtaggg atgacgggcg tccccataga tctgttcgcg
     2821 gctggcctgt tccatgaccc ggcccagata gagcaccatg acccggtggc tgacctccct
     2881 gaccacggcc aggtcgtggc tgatgaacat catcgccatg cccagctgtc gctgcaggtc
     2941 gatcagcagg tcgatgatct gggcgcggat cgagacatcc agcgcgctga cggcctcgtc
     3001 gcagatcacc agcttcggct cgaggatcat cgcgcgggcg atgccgaccc gctggttctg
     3061 gccgccgctc agttcgtgcg ggtagcggtt gacaagatcc ggcgccaggc cgcagcgctc
     3121 catcatcgcc gccgccgcct cccggcgcgc ctgccgcccg agaccgggcc tgaagacctg
     3181 cagcggctcg gcgatggaat cgccgatcgg catccgcgga tcgaggctcg ccaggggatc
     3241 ctggaagacg atctgcaggt ccttgcgcgc ggcccgcatg gccttgcggt cggcgtgggt
     3301 gatgtcgcgg ccgagcacgg tgaccgaccc gcccagctcc ccgccgcccg gcggcagcag
     3361 gttcagcgcc gcgcgagaca gggtggactt gccgcagccg ctttcgccga ccacgccaag
     3421 ggtttcgccc tggcggatct cgaagctgac tccgtcgacg gcgcgcaggg ggcgcggctt
     3481 gccgaacagg ccctggctga ccggaaacca gaccttgacg tcctcgcccc ggaccacgac
     3541 cggcgccgcc gccgccacgg gcgcaggggt tggccgcccg ccgcggtcgg gacggtccag
     3601 ccgcggaatc gccgccagca ggccgcgtgt gtagtcggtg cgcggctggt cgaacacggt
     3661 ttcgaccggg ccctcctcga cataggcgcc gtccttcatc acgcagaccc ggtcagccag
     3721 ccgggcgatg acgcccatgt cgtgggtcac caggaccatg ccggacccgg tctcgcgcat
     3781 cagctcggcc atcaggtcga gaatttcggc ctggaccgtc acgtcgagcg ccgtggtcgg
     3841 ctcatcggcg atcaacagcc tcgggccgca ggccatggcg ccggcgatca tcacgcgctg
     3901 ccgcatgccg ccgctcagct cgtgcggata ctggtccatc cgacgggcgg cctcggggat
     3961 gcggacgcga tcaagccatt cgcgggcccg ccgggccgcg tccgcgcccg acaggcccag
     4021 gtgcagccgc aggggttcgg cgatctgctc gccgatcttc acgtgcggcg tcagggccgt
     4081 cagcgggtcc tggaagatca tggtcatctt cgagccgcgg atgcggttca ggtcggccgg
     4141 ccgcaggccg agcagctcct gtccctcgaa acgggccgag ccggtcgccc gtccgttgcc
     4201 ggcgagcagg cccatcacgg tcatgaaggt ctggctcttg cccgaaccgc tctcgccgac
     4261 cacgcccagg cattcgccgg cggcgaccga cagggacacg ccgcggacgg cctcgacgag
     4321 gccgtcatgg gtctggaaac tgaccctcag gtcgtcgatc gacaatacgg gagcggacac
     4381 gggacaactc actcaggcgg ccaggccgga cgaccttagt gattgctggc tgacgcgcaa
     4441 atgaatgact gtggcggcgg cggtctcagg cgcggcgcgg aaagctggcc cagaagcggt
     4501 tgaagtcatg ggcgcagctg ggccgcgcga aggtcaccgc cttgtgcacg tgatccagca
     4561 gtggcgtgga ctgcatcagg gcgacggcat cggccatgcc ggtggcgtcg gcccgctcga
     4621 tgaagcggta tacggccgcg tcgaactggt cggcctcggg gccctcgggc aggcgaaact
     4681 caagcgtgac cacggaagcc ccctctgtct ctgattggca tactgcattg cggcagaagt
     4741 gttgcaacgc agaaaacgcc ggtttctcat cctttctgtc ttggcccgag gggcgatcgc
     4801 cgcggctccc atcgcggccc cggggcatgg aaggggcgcc cgtccgacgg gcgtctgtca
     4861 gtcctcggag accctcgggc attgccgcat gttgacttgg gataaaccaa tccggacctt
     4921 agatgggggg cgcaagggac cgtcggcggc gcgtccttat caagggggtc gatgtgagca
     4981 tctggcgcgc cacggcgccg gcgttgggcg gcaagggtcc gctcgccgca acgccgccga
     5041 ggatcatcgt ggtggccaac gagaagggcg gggcgggcaa gtcgaccgtg gccgccctgc
     5101 tgtcgaccgc catgctctac cagggccggc gggtcgcggt cgtcgatgtc gacctgcgcc
     5161 agcagaccct cagccatttc ttcgccaatc gccgtcgatg gctgcccgcc gccaatgtct
     5221 cggccccggt cccgctcgag tacatgctgg gcgacgaccc ggccgaactg tccgaggcag
     5281 atccggccaa ggtggttacc ctgttccacc gggcgctgac gctggcgatg gcggccgccg
     5341 atatcgtcgt catcgacacg ccgggcggcg atacgcccct ctcgagggcc gcccacctgc
     5401 aggcggacat cgtcgtcacc ccgatgaacg acagctttgt ggacttcgat ctgctcggga
     5461 agatcgatcc gatcacgctc aagctcgtac ggcccagcct ttacgcgaaa gtcgtccacg
     5521 acgcccgtcg cgcccgcgcc gatcatggcc gggcgctcga ctgggtcgtc cttcgcaacc
     5581 gcatggccgc caccgacggg cgcaaccggc agagggtgac cgtcaacctc gagcagctgg
     5641 ccggccaggt cggcttccgg ctcggacccc cgctccgcga gcggatcgcc tatcgcgaga
     5701 tgttcccgtt cggcctgacc ctggcggatc tctcggccaa cctgcggccc gccgaaatct
     5761 cgactccccg ccgcgccgcg cgcgaggaac tcaaggccct gctggtcgca ctgaacctga
     5821 tcgatgaggt ccagccggac gcggcggtgc tgaacagcgc tggctgagcc gaatggttac
     5881 ggacggttca ccgtgattga aaaccgcgcc ttaagcctga acatctagtg tccgtcgatg
     5941 tcggatgatc tgtccggcat tgggagatcg gtccatgttc gccagtctgg ggcgactggg
     6001 cggcgacagc gccgctcctc agcgatatcc gttcaggctc aagcgccggc cggaagagcg
     6061 tgtgaccctg ctgggccagg cgatggacct ggtgaaggct gaagaagtgc tgttcttcgt
     6121 ggcgaaccat gtggcccaga agaagcgcgc gatcgtcgcc aatcacaatg cccacagcct
     6181 gtacctcctg agggacgacg ctgagcttcg cgccttttat ggccgcgccg acctggttga
     6241 ggtggattcg cgccccctgc tgatgtgggc ccgcttgact ggacgcagca gtcgggcctt
     6301 ccaccggtgc acctatcttg attggcgtga cgagttctgg gccctggccg cggcgagagg
     6361 ctggcgtgtg tactatctgg gcgccgcccc cggtgtcgcg gagaaggcgg cggaattgct
     6421 gaggctgggg cggcccggtg tccggatcgg ggtgcacgat ggctatttcg acatgtcgcc
     6481 cggctctcct gaggccggcg cggtgcttga cgatatcagg gctttctcac ctgacgtatt
     6541 gatggtgggc atgggaatgc cgcgccagga ggtgtggatc catcgcaact acgacagcct
     6601 gcctccctgc gtcgttctgc cggtgggagc cgcgttcgac tatgaagccg gggtgcagcg
     6661 cgccgcgccg cgctggctgg gccagatcgg cctggaatgg ctgtttcgcc tggttgtcga
     6721 tccgcgtcgc ctgttccggc gctattgcgt agagccctgg cgtctcgtcg ggcttctggt
     6781 ggatgacgct gtcaactccg ccaggcagcg gcgggcgacg gcgctccgcg aacgccgccg
     6841 cgcgatcgcg ccgccccagg atgcgccacg acggagggcc tcggatcaac ggggtcgtgt
     6901 gaatctcgac ccgagcatcg gcggggttcc ggaactacag accggggcgg gcgctcgcag
     6961 acccgccgct gaccgccgcg tggcgacgct gtcgtcggaa ggctgacccc ggcgccgcgc
     7021 caccgggtcg acatcccggg aatcctgctt ctggtcgccg cgcgccgggc acggtagaag
     7081 atcacgagat gtcccagact cccgccgcgg cgccgccttt cgactctccc taccgacacg
     7141 gcttcgtgcg gctcgcggcc tgcgttcccc ggatcaccct cgccgacccg gcggccaacg
     7201 cccgcgaaac ccttgccctg ctgcgtcgcg gccacgaccg caacgtggcc ctgatggtct
     7261 ttccggaact gggactgtcg gcctattcaa tcgatgatct gctgcagcag gacgtgctgc
     7321 tcgatgcggt cgaggccgcg atcggccagc tcgtcgagtc gagccggagt ctcttcccgg
     7381 tcttcgccgt cggcgccccg ctgaggttcg acgggcggct gttcaacacc gcggtggtga
     7441 tccatcgcgg ggagatcctg ggcgtgatcc ccaagacctt cctgcccaat tatcgcgagt
     7501 actacgagcg ccgctggttc gcgagcggcc tgggcgtgac cggccagacc atcgcggtgg
     7561 ccggtcgggc cgcgccgttc gggaccgacc tgctgttccg ctcgaccgga tcggccccgt
     7621 tcacctttca cgccgagatc tgcgaggacg tctggacgcc cgagcctccc tcgaccctgg
     7681 cggcgctggc cggcgccgag atcctgctca acctgtcggc cagcaacatc gtcatcggca
     7741 aggccgaggc gcgacgcctg ctgtgcgcgt cgcagtcgat gcgctgccag gcggtctacg
     7801 cctattcggc ggccggagtc ggcgagtcga cgacggacaa cgcctgggac ggccaggcct
     7861 cgatcttcga gctgggcgaa ctgctggtcg agagcgaacg cttcccggcg gagtcgacgc
     7921 tggcgatggt cgacgtcgac ctcggccggg ttcgccagga gcggttccgc accggatcgt
     7981 tcggcgacag cgtcgcccgg cgccagtcga acacgccctt ccgcgtccag accttccgcc
     8041 tggacgcgcc gacgacgacg ctggcgctgg cgcgggccgt gccgcgattc ccgttcgtgc
     8101 ccgacgacga gggccgcctc gccgaacagt gccacgaagc ctacagcgtg caggtccagg
     8161 gcctggccca gcgactccgg tcgaccgggc tcgagcggct ggtcatcggc gtgtcgggcg
     8221 gtctcgattc gacccaggcc ctgctcgtgg cctgcaaggt catggatcag ctcggtctgc
     8281 cgcgcgccaa catcctcgcc ttcaccatgc ccggcttcgg caccagcgag cggacccggg
     8341 ccaacgcctg gacattgatg aacaccctgg gcgtgacggc cgcggagctc gatatccgcc
     8401 ccgccgcgac ccagatgctc ggtgatctcg gccatccgtt cgccgagggc gagccggtcc
     8461 atgacgtgac gttcgagaac gtccaggccg ggctgcgaac ggactacctg ttccgcatcg
     8521 ccaaccagcg gggcgggctg gtggtcggga ccggtgacct gtcggagctg gcgctgggct
     8581 ggtgcaccta cggcgtcggc gaccacatga gccactacaa tccgaacggg tctgtgccga
     8641 agaccttgat ccagcatctg atccgcttcg tcgccgccgc cggcgagacc ggcccggccg
     8701 gggcggcggt cctcggcgac atcctcgcca cggagatatc gcccgagctt gtcccggccg
     8761 gcgcggacgg caggggccag gccaccgagg atgtggtcgg cccctatgcc ctgcaggact
     8821 tcaacctgtt ccacctgacc cgctacggca tggcgccgtc gaagatcgcc tatctggcca
     8881 tcgcggcctg gagcgacgtc tcgcggggcc agtggccgcc cggattgccg gccggcgacc
     8941 gccgcgccta cggcgccgag gaaatcacgg cctggctgaa ggtcttcctc aggcggttct
     9001 tcgccaacca gttcaagcgg tctgcggtcc cgaacgggcc gaagatctcc tcgggaggcg
     9061 cattgtcgcc gcgcggcgac tggcgcgcgc cgtcggacgc cggtccccgg gcctggctcg
     9121 aggagctcgg cgcgaacggg cctgcgggga atggctgagg cttcgcggac acacaggtcg
     9181 aacatcggtc ggccaagcgc tggataatcg cgtgaaggtg gcgtatggtc cggccagcgc
     9241 gtgggaaacc acggttgaaa atggatcggg agcttgcgtg gaacccttta tcggttcatg
     9301 cgtttcttga tcgggctgaa cggcggctac atgttcgggg gatggggtgt gcccgatcca
     9361 gaccgggcgt ggggaagcca gtcatccatg gttggcgccg gccgttcggc gtgtggttat
     9421 aagactagag cgaggggctc atcgtgaaga aaaaggcact gttgctgggc acaatgctcg
     9481 gcgcgctggc gctggcgcct caggcgttcg cgcagtccac cgggctgtat ggagccttcg
     9541 atctcggcta ccaccaaccc gatgatcttg acgtccactc ggccgtgttc ggcacttccg
     9601 ccaatgtcgg ccttgaaggt gacatcgtcg ggttcgcccg cctcggctac cggatcatgc
     9661 cgaactggcg tgtcgagctt gaaacgggcc tgcgccctgg ggatatcgac gaggttccca
     9721 cggatatcaa cggccacttc caggccctgt cgatcatggg caacgtgctc tatgacttcg
     9781 cgcctgacgc cagcatcaac ccgttcttcg gcgtcggcgc gggtgtcgtg cagggtcacc
     9841 tggctgccgt gaccccgtcg gtttggctgg ttgatagcga cgaccgcgac agcgccttcg
     9901 cctaccaggt gctggcgggt ctgtcggcgc cgctgagcga ccagctgtcg atggacttca
     9961 cctatcgcta tatcaaggcg acggatatca agatcgagaa cccggccttg gccgggccgc
    10021 tcgaaattgg ttacgaagac cagtcggtga ccttcggcct gcgttacagc ctgcaacagg
    10081 aagcggcccc gccgccgccg ccgccgccgc ctccgccccc gccgcctccc ccgcctcc
//
LOCUS       DAORNX010000050        10124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_4927_length_10124_cov_8.712186, whole genome shotgun sequence.
ACCESSION   DAORNX010000050 DAORNX010000000
VERSION     DAORNX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4927_length_10124_cov_8.712186"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1989
                     /locus_tag="PLO65_03640"
     CDS             301..1989
                     /locus_tag="PLO65_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRD27374.1"
                     /translation="MRVNRRGLLALLGAGAGAAATAAEARSAGAVRFDHGVASGDPLA
                     ESLIFWTRVTPTDGAADPVSGRLKYALNAMDLDSPDQFWSMDVTASPDRDWTVKVDVT
                     GLRAATDYVFRFEFGGVKSPVGRARTLPTGPTADVVLAVASCALYPNGYFNAYGAIAG
                     LERVDAVVHLGDYIYEYGGPGSYGADSPVAAQRPHDPPHECLSLADYRRRHAQYKADP
                     QLQAAHARASWIVVWDDHEVCNDSWKGGAENHNPDTGEGDWAARKAAALKAYYEWMPI
                     REPGPGRSAEMANRSFQFGDLASLLMVETRLTARSHQLSYGEDLPIVDGKPDAAAFKA
                     KWLDPERRLIGPDQLAWLGAELAGSVAAGRTWQILGNQIVMGRVASPDFKTQMGDQKF
                     GEMVAGLPEEYQKPVAQLSALGAFGLPYNLDAWDGYPVDRERVLGAFKATGAHPLVLA
                     GDSHAFWANALHDAGGTLVAAEFGTTGVTSPGAGDVIKGVPLNQGFEDKNPEVVFTDH
                     SAKGFVLLTLTRAEARAQFMAVSTIESRDYTASALRTYSVTPVTGGGVSTLKAG"
     gene            complement(2107..2508)
                     /gene="hisI"
                     /locus_tag="PLO65_03645"
     CDS             complement(2107..2508)
                     /gene="hisI"
                     /locus_tag="PLO65_03645"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRD27375.1"
                     /translation="MSEPLFPTAPDTAALERGAVLSPRFDAAGLIAAIAQHADSGEVL
                     MLAWMNAEALKLTLETGQAHYFSRSRNALWKKGETSGQLQDVVELRIDCDQDAVLIKV
                     RPRGDGGACHVGFRSCFYRTLVQDGTLEERG"
     gene            2703..3587
                     /locus_tag="PLO65_03650"
     CDS             2703..3587
                     /locus_tag="PLO65_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HRD27376.1"
                     /translation="MNLNARIKALVANLDLRIRPWAERSRWRGWAYEFLLFGLKQAWA
                     CLFGGLMLALLVGTRLLWPGDAAMHRYDFLVVAALAIQFGLLALRLERPDEALVILIF
                     HIVGTVMEIFKTAHGSWVYPEASLMRIGGVPLFSGFMYACVGSYIARAWRLFDIRFEQ
                     YPPGWTTWALAAAAYVNFFSHHYVQDVRWPLFVASAAIFGRSWFVFTPDRAARRMPML
                     LGFMLVALFIWLAENLGTFANAWRYPTQADIWRPVGLAKMGSWYLLMLLSFVLVSAVH
                     RPKGTPTEGACPPNERQV"
     gene            complement(3623..4255)
                     /gene="folE"
                     /locus_tag="PLO65_03655"
     CDS             complement(3623..4255)
                     /gene="folE"
                     /locus_tag="PLO65_03655"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRD27377.1"
                     /translation="MDAMSRERTLIGGAGLDLEPAKRPTREEAMEAVRTLIAWSGDDP
                     RREGVIDTPKRVVDAYGEWFEGYAADPAKELSRTFEDVQGYDDIVMLREIEVESHCEH
                     HMAPFLGKAYVAYMPTNRVVGISKIARVVEIFARRLQTQETMTQQITDAICDSLKPAG
                     VAVLIDAAHQCMTTRGVHHRHVTTITTQFRGVFKTDATLRQRFLDFVNRG"
     gene            4423..5799
                     /gene="cysS"
                     /locus_tag="PLO65_03660"
     CDS             4423..5799
                     /gene="cysS"
                     /locus_tag="PLO65_03660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRD27378.1"
                     /translation="MSLKLYDTMAREKRPFVPADPERVTMYVCGPTVYGYAHIGNARP
                     VVVFDVLFRLLRHLYGQDAVIYARNVTDVDDKINRKAADEGVDIRVITDRYLAAYEAD
                     MGALGALTPTLQPRATENIAAMLDQIGALIQRGCAYAAEGHVLFDTQSFPDYGRLSGR
                     PLDEMIAGARVDVAPYKRHPADFVLWKPSKPGEPEWPSPWGAGRPGWHIECSAMIEAT
                     LGLPIDIHGGGIDLQFPHHENELAQGVCAHGGGEYARYWMHNGFLDMAGEKMSKSLGN
                     VVIPHDLLESVPGEVLRWALLSAHYRQPLDWTGELIEQSRKALDRLYGALQRVKGLDV
                     EPRDPPAAFLEALGDDLNTPKAYAELFALAKTLETAHGDGKAAAKADLLAAANLLGFL
                     EADPDVWFAGGADDDLRGKVEALLEARVAARAGKDWPEADRIRGELDALGVVVMDSPT
                     GATWRLKE"
     gene            5850..6086
                     /locus_tag="PLO65_03665"
     CDS             5850..6086
                     /locus_tag="PLO65_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27379.1"
                     /translation="MLEDAAGSRGCHHGLGPRLRGFTAKYDVNRLVWYEVHDTREAAK
                     SCERRIKKWKRAWKIRLIEETNPGWLDLYETWNC"
     gene            6137..6586
                     /locus_tag="PLO65_03670"
     CDS             6137..6586
                     /locus_tag="PLO65_03670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD27380.1"
                     /translation="MAVKLERRIGIQAPDEIVWEILSDIPGWGSWTGLYPKAQGEIRI
                     GNRWTVELALPGQAPRTIHPVILDWAPYDHIHWRLDVMRGWARTVRFLEIEKMGPENS
                     IFSNGEIFDGLLGPTIARRLRRPVIESFEAMNEELKQRSEALWAARR"
     gene            6681..7133
                     /locus_tag="PLO65_03675"
     CDS             6681..7133
                     /locus_tag="PLO65_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRD27381.1"
                     /translation="MIDDLYSARILTLAANMPHAGRLAAPQGSAEKVAKLCGSRIVVD
                     VDLDAAGKVAAFAQDVQACALGQAAAAVLGAHVIGAGLDEIETARDQLAAMLKSGGPA
                     PTGRFEDLAVLAPVKDYPARHASTLLAFEAAVAAVRQASARTSGADAA"
     gene            7192..7431
                     /gene="yidD"
                     /locus_tag="PLO65_03680"
     CDS             7192..7431
                     /gene="yidD"
                     /locus_tag="PLO65_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRD27382.1"
                     /translation="MTLYERSVTLAHRAYKLTLSPLIGRQCRFLPTCSDYAREALITH
                     GPVRGGWLATKRLCSCHPWGRSGFDPVPPRKCDSP"
     gene            7428..9347
                     /gene="thrS"
                     /locus_tag="PLO65_03685"
     CDS             7428..9347
                     /gene="thrS"
                     /locus_tag="PLO65_03685"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRD27383.1"
                     /translation="MIDLIFPDGSVRQYPQGSSGRDVAQAISPSLAKRTLLVRLDGQL
                     LDFDRPLPGGGKVEFVDRNDPDALDTIRHDTAHVLAEAVQELFPGTQVTIGPNVEDGF
                     YYDFARAEPFSTDDFAAIEKRMAEIVDRDEKITREVWERDKAIAHFKAIGEDYKAEII
                     EGIPADQDVTLYRQGAWADLCRGPHLPSTKHVGKAFKLTKLAGAYWRGDQNNAQLQRI
                     YGTAWASQEDLDAYLKRVEEAEKRDHRKVGRQMDLFHMQEEGRGMVFWHPKGWTLWRT
                     LEAYMRRRLDAAGYIEVKTPQVLERTFWENSGHWDKYRPNMFVCETVEGEVLSLKPMN
                     CPGHVQIFGVGQRSYKELPMRMAEFGACHRYEPSGALHGLMRVRAFTQDDAHIFCRED
                     QIVDETERFIRLTKMIHADLGMETKYIALATRPEIRAGSDAFWDKAEGMLAEAARLAG
                     VEPVIAEGDGAFYAPKLDFIVQDAIGREWTCGTLQLDYVLPERLGAEYVAEDGSKQRP
                     VMLHRAILGSFERFIGIMIENYAGRFPMWLAPVQAVVTTIVSEADDYAGEVAAKFRAA
                     GLRVETDLRNEKVGYKIREHSVAKVPAIAVVGRKEAEEGTVAIRRLGSQEQQVVSVDE
                     AIRLLTEEATPPDLR"
     gene            9517..>10124
                     /locus_tag="PLO65_03690"
     CDS             9517..>10124
                     /locus_tag="PLO65_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27384.1"
                     /translation="MPSFTAADGVEIAYDDTGGPGAPLLLIHGFSSNRQEAWKRTGWY
                     AAIAQRGQRLVALDLRGHGESGKPHDPAAYGRDILRADIIGLLDHLGVEWANVFGYSM
                     GARLALSLAIHERERVGYLILGGVGGTLFDPPVAGDPMALAMEADSPDSISEPMLRSF
                     RQFADEQGEDRLALAAFVRAAVEPVTRDAVQAVSAPTLVAAGA"
BASE COUNT         1580 a         3428 c         3515 g         1601 t
ORIGIN      
        1 cctcgtcatc gcagtcgtcg gcccgttgac gataccagtc gaccgcctcg agctcctcga
       61 tcagggaggt gatcgcatag tgctgcagca gggtctttcg actcagcttc tcgcgcggaa
      121 cgtgaaggcc ttcactcgac atacggggct cccggccggg gttgaacgga ccggactctc
      181 ataacaggtc gtggccggcg gcggggccgt ccgcgcgctt ttttcgcacg gcgctggaca
      241 ggcgtcatgg cgccgttgtg aactcccgcc aaagtgggaa gccagcgggg ggaatcggac
      301 atgcgggtta acaggcgggg actgctggct ctgctgggcg ccggcgcggg cgcggcggcg
      361 accgccgcgg aggccaggtc ggccggcgcg gtccggttcg atcatggcgt ggccagcggc
      421 gatcccctgg ccgaaagcct gatcttctgg acccgggtga cgccgacgga cggcgccgcc
      481 gatccggtgt cgggccggct gaagtacgcc ctcaacgcca tggacctcga cagtccggac
      541 cagttctggt cgatggatgt caccgcgagc ccggatcggg actggaccgt caaggtcgac
      601 gtcacgggcc tgcgcgcggc gaccgactat gtgttccgct tcgagttcgg cggcgtgaag
      661 tccccggtgg gccgggcccg caccctgccg acggggccga cggcggatgt cgtgctcgcg
      721 gtggcctcct gcgcgcttta ccccaacggc tacttcaacg cctacggggc gatcgccggg
      781 ctcgagcggg tcgacgccgt cgttcacctg ggcgactaca tctacgaata tggcggaccc
      841 gggtcctacg gggcggactc gccggtcgcg gcgcaacggc cgcatgatcc gccgcacgaa
      901 tgcctgtcgc tggccgacta ccgtcgccgc catgcccagt acaaggccga tccgcagctg
      961 caggcggccc acgccagggc ctcctggatc gtcgtgtggg acgaccacga ggtctgcaac
     1021 gacagctgga agggcggggc ggagaaccac aatcccgaca cgggtgaggg cgactgggcg
     1081 gcgcgcaagg cggcggccct gaaggcctac tacgagtgga tgccgatccg cgaaccgggg
     1141 cccggacggt cggcggagat ggccaaccgg tcgttccagt tcggcgacct ggcctcgttg
     1201 ctgatggtcg agacccggct gaccgcccgg tcgcaccagc tgagctacgg cgaggacctg
     1261 ccgatcgtcg acggcaagcc cgacgcggcg gcgttcaagg cgaagtggct cgatcccgaa
     1321 cgccggctga tcgggcccga ccagctggcc tggctcggcg ccgagctggc cggttcggtg
     1381 gccgcgggcc gcacctggca gatcctcggc aaccagatcg tcatgggccg ggtggccagt
     1441 ccggatttca agacgcagat gggcgaccag aaattcggtg aaatggtcgc cggcctgccc
     1501 gaggagtatc agaagccggt ggcacagctg tcagcgctgg gtgccttcgg cctgccctac
     1561 aatctcgacg cctgggacgg ctatccggtc gatcgggagc gggtgctggg cgcgttcaag
     1621 gcgaccgggg cccatccgct ggtgctggcc ggcgacagcc acgccttctg ggccaacgcg
     1681 ctgcacgacg cgggcgggac gctggtggcg gcggagttcg gcacgacggg tgtcaccagc
     1741 cccggcgccg gggacgtgat caagggcgtg ccgctgaacc agggcttcga ggacaagaat
     1801 cccgaggtcg tgttcaccga tcacagcgcc aaggggttcg tgctgctgac gctgacccga
     1861 gccgaggcga gggcgcagtt catggccgtc tcgacgatcg agtcgcgcga ctacacggcg
     1921 tctgcgttgc ggacctatag cgtaacgccc gtgaccggcg gcggggtgag cacgctgaag
     1981 gcggggtgac ccaaccgctc atccccggac agtcgaaccc gtcatcctcg ggcttgtccc
     2041 gaggactgac gcgcgcgcgg cgttcctggg gcgtcgggac aggcccgacg aagacggtga
     2101 ggggggctat ccccgctcct caagcgtccc gtcctgcacc agggttcgat agaagcagga
     2161 ccggaagccg acgtgacacg caccgccgtc tccgcgtggt cgcactttga tcagcaccgc
     2221 gtcctggtcg cagtcgatgc gcagttcgac gacgtcctgc agctggccgc tggtctcgcc
     2281 cttcttccac agggcgttgc gcgagcggct gaaatagtgc gcctgtccgg tttcgagggt
     2341 cagcttgagc gcttccgcat tcatccaggc cagcatcagc acctcgccgg agtcggcgtg
     2401 ctgggcgatg gcggcgatca ggcccgccgc gtcgaagcgg ggcgacagca ccgcgccgcg
     2461 ctccagcgct gcggtgtcag gggcggtcgg gaagaggggt tcgctcatgc gcgcatatgg
     2521 ccccagggcg cggaaggcga caaccgctca tcctccccca cctgggggag ggggatgcgc
     2581 cgctgatggt cgggcgcgca ccaggttgcg tcgcgtcggg gcccgcgcat gatgatccaa
     2641 caccacgcca cccactcgac catgcttcgc atggtccccc tctcccaggt gggagaggag
     2701 agttgaatct caacgcccgc atcaaggcgc tcgtcgccaa cctcgacctg cgcatccggc
     2761 cctgggcgga gcgctcgcgc tggcgcggct gggcctacga gttcctgctg ttcggcctga
     2821 agcaggcctg ggcctgtctg ttcggcgggc tgatgctggc cctgctggtc ggcacgcggc
     2881 tgctgtggcc gggcgacgcg gcgatgcatc gctacgactt cctcgtggtc gccgccctgg
     2941 cgatccagtt cggtctgctg gccctgcgcc tggaacgccc ggacgaggcg ctggtgatcc
     3001 tgatcttcca catcgtcggc acggtgatgg agatcttcaa gacggcccac ggctcatggg
     3061 tctatcccga ggccagcctg atgcggatcg gcggcgtgcc gctgttctcc ggcttcatgt
     3121 acgcctgcgt cggcagctat atcgccaggg cctggcggct gttcgacatc cgcttcgagc
     3181 agtacccgcc gggctggacc acctgggccc tcgcagccgc ggcctacgtg aatttcttca
     3241 gccaccacta tgtgcaggat gtccgctggc cgctgttcgt cgcgtcggcg gcgatcttcg
     3301 gccggtcgtg gttcgtgttc acgccggacc gggcggcgcg gcgcatgccg atgctgctgg
     3361 gcttcatgct ggtggcgctg ttcatatggc tggccgagaa cctcggcacc ttcgccaacg
     3421 cctggcgcta tccgacgcag gccgacatct ggcggccggt cggcctggcc aagatgggca
     3481 gctggtacct gctgatgctg ctgagcttcg tgctggtgtc agcggtgcat cggccgaagg
     3541 ggacacctac cgaaggtgcg tgtccgccaa acgagcggca ggtttagggg acacgcacct
     3601 tcggtacatg tccccgtcga acctagcccc ggttcacgaa gtccaggaac cgctggcgca
     3661 gggtcgcgtc ggtcttgaac acgccgcgga actgggtggt gatggtcgtc acgtggcggt
     3721 ggtgcacgcc gcgcgtggtc atgcactggt gagccgcgtc gatcagcacg gccacgccgg
     3781 ccggcttgag actgtcgcag atcgcgtcgg tgatctgctg ggtcatggtc tcctgggtct
     3841 gcagccggcg ggcgaagatc tcgaccaccc gggcgatctt gctgatgccg accacgcggt
     3901 tggtcggcat gtaggcgacg taggccttgc ccaggaacgg cgccatgtgg tgctcgcagt
     3961 ggctctccac ctcgatctcg cgcagcatga cgatgtcgtc gtagccctgc acgtcctcga
     4021 aggtccggct cagttccttg gccgggtcgg cggcgtagcc ctcgaaccac tcgccgtagg
     4081 cgtccaccac gcgcttgggc gtgtcgatca cgccctcgcg gcgggggtcg tcgccggacc
     4141 aggcgatcag ggtgcggacc gcctccatcg cttcctcgcg cgtcgggcgc ttggcgggtt
     4201 caagatcaag accggcgccg ccgatcaggg ttcgctcgcg gctcatggcg tccatggttg
     4261 ctgagggtct tcccggggct gagttgcgcc ggaacgcctg tcgttccgga atgacgcgtg
     4321 ccaccccatt cacagcgcga cggaccgtgg cctcgcccgc gcctgaaagc tatatggggc
     4381 caatagccga cccggcaagc tcttaccgaa cgtcaatcac ccatgtccct gaagctctac
     4441 gacaccatgg cccgcgagaa gcggcccttc gttcccgccg atccggagcg ggtgacgatg
     4501 tatgtctgcg ggcccacggt ctacggctac gcccacatcg gcaacgcccg cccggtggtg
     4561 gttttcgacg tgctgttccg cctgctgcgc cacctctacg gccaggacgc ggtgatctat
     4621 gcgcgcaacg tcacggacgt ggacgacaag atcaacagga aggccgccga cgagggcgtc
     4681 gacatccgcg tgatcaccga ccgctacctg gcggcctatg aggcggacat gggcgcgctg
     4741 ggcgccctga cgccgacgct gcagccgcgc gcgaccgaga acatcgcggc gatgctggac
     4801 cagatcggcg cgctcatcca gcgcggctgc gcctatgcgg ccgaggggca tgtgctgttc
     4861 gacacccaga gcttccccga ctacggccgc ctgtcgggcc ggccgctgga cgagatgatc
     4921 gccggcgccc gggtcgatgt cgccccctac aagcgccacc cggccgactt cgtgctgtgg
     4981 aagccgtcca agcccggcga gcccgaatgg cccagcccgt ggggcgcggg ccggcccggc
     5041 tggcacatcg agtgctcggc aatgatcgag gccactctcg gcctgccgat cgacatccac
     5101 ggcggcggca tcgacctgca gttcccgcac catgagaacg aactggccca gggggtctgc
     5161 gcccacggcg gcggcgagta cgcccgctac tggatgcaca atggcttcct cgacatggcc
     5221 ggggagaaga tgagcaagag cctcggcaac gtcgtcattc cgcacgacct gctggagtcc
     5281 gtgccgggcg aggtgctgcg ctgggccctg ctgagcgccc actaccgcca gccgctcgac
     5341 tggaccggcg agctgatcga gcagagccgg aaggccctcg accggctcta cggcgccctg
     5401 cagcgggtga agggtctgga tgtagagccg cgcgatccgc cggcggcctt cctcgaggcg
     5461 ctgggcgacg acctcaacac gcccaaggcc tacgccgagc tgttcgccct ggccaagact
     5521 ctggaaaccg cccacggcga cggcaaggcg gcggccaagg ccgacctgct ggcggccgcc
     5581 aacctgctgg gcttcctgga ggccgatccg gacgtgtggt tcgccggcgg ggccgacgac
     5641 gacctgcgcg gcaaggtcga agccctgctc gaggcccgcg tcgcggcccg cgccggcaag
     5701 gactggcccg aggctgaccg catccgcggc gaactggatg ctttgggtgt cgtggtgatg
     5761 gactcaccca cgggggcaac ctggcgattg aaggagtagt tcggcctccc ccagagccga
     5821 ttgtccccgc gaaggcgggg acccaagcga tgcttgagga cgctgcgggt tcgcgcggct
     5881 gccaccacgg cttgggtccc cgccttcgcg ggttcactgc caagtacgat gtcaatcgtc
     5941 tcgtctggta tgaggtccac gacacccgcg aggccgccaa gtcgtgtgag cgacggatca
     6001 agaaatggaa acgggcctgg aaaatccggc tgatcgagga aaccaatccc ggttggctcg
     6061 atctatacga aacgtggaat tgctgattga accaccgcct gggtcccggc cttcgccggg
     6121 atgagcggat tgagagatgg cagtaaaact tgagcgccgc atcggcatcc aggcccccga
     6181 cgagatcgtc tgggagatcc tgtcggacat tcctggctgg gggagctgga ccgggctcta
     6241 tccgaaggcc cagggcgaga tccgcatcgg caatcgctgg acggtcgagc tggcgctgcc
     6301 cgggcaggcg ccgcggacga tccatccggt gatcctcgac tgggcgccct atgaccacat
     6361 ccactggcgg ctggacgtga tgcgcggctg ggcgcggacg gtgcggtttc tcgagatcga
     6421 gaagatgggg ccggagaact ccatcttctc caacggcgag atcttcgacg gcctgctcgg
     6481 cccgaccatc gcccggcgtc tgcgccggcc ggtcatcgag agcttcgagg cgatgaacga
     6541 agagctcaaa cagcggtcgg aagcgctgtg ggcggcgcgg aggtagcgct cggggacatg
     6601 cacttcagtg cgtgtccgct ttatcggtgg gggacacgca ctgaagtgca tgtccccgtg
     6661 tttgtgacta tctaggcccc atgatcgacg acctctacag cgcccgtatc ctgaccctgg
     6721 cggccaacat gccgcacgcg gggcggctgg ccgcgcctca ggggtcggcc gagaaggtgg
     6781 ccaagctgtg cggcagccgg atcgtcgtcg acgtcgacct ggacgccgcc ggcaaggtcg
     6841 cggccttcgc ccaggacgtg caggcctgcg ccctcggcca ggccgccgcg gccgtgctgg
     6901 gggcccatgt catcggcgcc ggacttgacg aaatcgaaac cgcgcgcgat cagcttgccg
     6961 cgatgttgaa gtccggcggc cccgcgccga ccggacggtt tgaggatctg gccgtgttgg
     7021 cgcccgtgaa ggactatccc gcccgtcatg cctcgacgct cctggcgttc gaggccgcgg
     7081 tcgcggctgt ccgccaggcg agcgcgcgaa ctagcggcgc cgacgcggct tgatcgcgcg
     7141 cgctggcgcg tcgataagcg ccgggactcg ttcacccacc ctcgtgtcgg catgaccctc
     7201 tacgaacgca gcgttacgct ggcgcaccgc gcctacaagc tgacgctgtc cccgttgatc
     7261 ggacgccagt gccgcttcct gccgacctgt tcggactatg cgcgcgaggc tttgatcacg
     7321 cacggaccgg ttcgtggcgg ctggctcgcc acgaagcgcc tgtgcagctg ccatccctgg
     7381 ggccgctccg gattcgatcc ggtgccgccg cgaaagtgtg actcgccatg atcgacctga
     7441 tcttccccga cggctccgtc cgtcagtatc cgcaaggctc cagcggccgc gacgtggccc
     7501 aggccatctc gccctcgctg gccaaacgca cgctgctggt ccgcctcgac ggccagctgc
     7561 tcgacttcga ccgcccgctg ccgggcggcg gcaaggtcga gttcgtcgac cgcaatgatc
     7621 ccgacgccct cgacaccatc cgccacgaca cggcccacgt gctggccgag gccgtgcagg
     7681 agctgttccc cggcacgcag gtgaccatcg gcccgaacgt cgaggacggc ttctactacg
     7741 acttcgcccg cgccgagccg ttcagcacgg acgacttcgc ggcgatcgaa aagcgcatgg
     7801 ccgagatcgt cgaccgcgac gagaagatca cgcgcgaggt gtgggagcgc gacaaggcca
     7861 tcgcccactt caaggccatc ggcgaggact acaaggccga gatcatcgag ggcatcccgg
     7921 ccgatcagga cgtgacgctc tatcgccagg gcgcctgggc cgacctctgc cgcgggccgc
     7981 acctgccgtc gacgaagcac gtcggcaagg ccttcaagct gaccaagctg gccggcgcct
     8041 actggcgcgg cgaccagaac aacgcccagc tgcagcgcat ctacggcacg gcctgggcca
     8101 gccaggagga cctcgacgcc tatctcaaac gcgtcgagga ggccgagaag cgcgaccatc
     8161 gcaaggtcgg ccggcagatg gacctgttcc acatgcagga agaaggccgc ggcatggtct
     8221 tctggcatcc caagggctgg acgctgtggc gcacgctcga ggcctacatg cgccgccgtc
     8281 tcgacgccgc cggctacatc gaggtcaaga cgccccaggt gctggaacgg acgttctggg
     8341 agaactccgg ccactgggac aagtaccggc ccaacatgtt cgtctgcgag acggtcgagg
     8401 gcgaggtgct gtcgctcaag ccgatgaact gcccgggcca cgtgcagatc ttcggcgtcg
     8461 gccagcgctc ctacaaggag ctgccgatgc gcatggccga gttcggggcc tgtcaccgct
     8521 acgagccgtc gggggccctg cacgggctca tgcgcgtgcg cgccttcacc caggacgacg
     8581 cccacatctt ctgccgcgag gaccagatcg tcgacgagac ggaacggttc atccgcctga
     8641 cgaagatgat ccacgccgac ctcggcatgg agacgaagta catcgcgctc gcgacacggc
     8701 cggaaatccg cgccggttcc gacgcgttct gggacaaggc cgagggcatg ctggccgagg
     8761 ccgcgcgcct cgccggtgtc gagccggtga tcgccgaggg cgacggggcc ttctatgcgc
     8821 ccaagctgga cttcatcgtc caggacgcaa tcggtcgcga gtggacctgc ggcacgctgc
     8881 agctcgacta tgtgctgccc gagcggcttg gcgccgaata cgtcgccgag gacgggtcga
     8941 agcagcggcc ggtcatgctg caccgggcca tcctcggctc gttcgagcgg ttcatcggca
     9001 tcatgatcga gaactacgcc ggccgtttcc cgatgtggct ggcgccggtc caggccgtgg
     9061 tcacgaccat cgtctccgag gctgacgact atgccggcga ggtggccgcg aaattccgcg
     9121 ctgccggcct gcgggtcgag accgatctgc gcaacgagaa ggtcggctac aagatccgcg
     9181 agcacagcgt cgccaaggtg ccggcgatcg cggtggtcgg ccgcaaggag gccgaggaag
     9241 gcacggtcgc cattcgccgc ctgggttcgc aggagcagca ggtggtgtcg gtggatgaag
     9301 ccatccgtct gctgaccgag gaagcgacgc cgcccgatct gcggtagtcg agcgggggca
     9361 tgcacttcag tgcgtgaccc catcggcttc aaggcggcgg gggacacgca ctgaagtgca
     9421 tgtcccctcc cggtttccgc ttgacccccg cccgccacac gccaccctgt cctcgcgggt
     9481 ccgcagagag tcgcgccggg aacagggacg acccggatgc ccagcttcac cgccgccgac
     9541 ggcgtcgaga tcgcctatga cgacaccggc ggccccggcg cgcccttgct gctgatccac
     9601 gggttctcgt cgaaccggca ggaggcctgg aagcggaccg gctggtacgc ggccatcgcc
     9661 cagcggggcc agcgcctggt cgcgctcgac ctgcgcggcc acggcgagag cggcaagccg
     9721 cacgatccgg ccgcctatgg ccgcgatatc ctgcgggccg acatcatcgg tctgctcgac
     9781 catctgggcg tcgagtgggc gaatgtgttt ggctattcga tgggcgcgcg gctggccctt
     9841 tcgctggcga tccacgaacg cgagcgggtc ggctacctga tcctcggcgg cgtgggcggg
     9901 acgctgtttg acccacccgt cgccggcgat ccgatggccc tcgccatgga ggcggacagt
     9961 cccgacagca tcagcgagcc gatgctcagg agcttccgcc agttcgccga cgagcagggc
    10021 gaggatcgcc tggccctggc ggccttcgtg cgcgcggcgg tcgagccggt cactcgcgac
    10081 gccgtgcagg ccgtctcggc gccgaccctg gtcgcggccg gggc
//
LOCUS       DAORNX010000051         9790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5123_length_9790_cov_7.692450, whole genome shotgun sequence.
ACCESSION   DAORNX010000051 DAORNX010000000
VERSION     DAORNX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_9790_cov_7.692450"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..997)
                     /locus_tag="PLO65_03695"
     CDS             complement(272..997)
                     /locus_tag="PLO65_03695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD27385.1"
                     /translation="MTLDLIDAPSPNFDARTKVPDMLVLHYTGMKSGPEALARLRDPE
                     ARVSSHYMVEEDGRIFRLVAEERRAWHAGRSFWKGEEGVNHASIGIEIVNPGHEFGYR
                     PFPEAQIAAVIALLTDIRGRWSIEDDRILGHSDVAPARKQDPGELFPWKQLAQAGHGV
                     WAETDPAPGEPLAEGAEGVGVFALQAGLTRLGFDCAPTGRFDAGTREVVTAFQRHWVQ
                     SRADGVADGLTRARLMAVLRASG"
     gene            complement(994..1887)
                     /locus_tag="PLO65_03700"
     CDS             complement(994..1887)
                     /locus_tag="PLO65_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD27386.1"
                     /translation="MALRDFALLVVMCFVWATNTIVSKIVIAHWGVPPLYYAGARFVV
                     VMLCTLPWLFPAPRPLWRMIIVCQLIGGANFALMFMALKTASPSAASIVIQLGVPMTT
                     VLSMIMLGEQVRWRRGIGMALTLAGAVAVMWRPGGMALSLGLWLVAAAALAGALGAVM
                     MKQMEGVRPLQFQAWVGFTSAIPLLGFSALTEQGQWAATLAAGWPLLAAVLYSAIVIS
                     VIAHTAYYGLIQKYEATLISPLTLLTPLFTIVLGVLVTHDHFDARMAIGATLALAGVL
                     IIALRPNHVMPLLLLMRNRFQ"
     gene            2094..2540
                     /locus_tag="PLO65_03705"
     CDS             2094..2540
                     /locus_tag="PLO65_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD27387.1"
                     /translation="MKEVDFKDLASLVGQEIGVSDWVQITQERVNQFAEATGDHQWIH
                     VDVERATREMGGPIAHGYLTLSLIPFLGAGLLRVKGVTRGINYGSDKVRFTNMVRVGK
                     KVRMRQKLLSVEPKAGGLQMKNECTIEIEGEERPACVAETISVIYG"
     gene            complement(2619..2837)
                     /locus_tag="PLO65_03710"
     CDS             complement(2619..2837)
                     /locus_tag="PLO65_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRD27388.1"
                     /translation="MWDRIEGSWKEMSGKVKEQWGKLTDDDLTVVAGKRDQLAGKIQE
                     RYGYAKDKADEEISAFERRMDEDRSFRS"
     gene            complement(2902..3723)
                     /locus_tag="PLO65_03715"
     CDS             complement(2902..3723)
                     /locus_tag="PLO65_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRD27389.1"
                     /translation="MAWDLSVDLIKATVQLEQPLGDGTRTVGTGFLVNAPTPDGKPRV
                     VLVTAGHVFDRMPIASARIGFRAQTSDGGWRYDPEPLTIRDDGKPLWTKHPDRDVAAI
                     VIKAPPEFAQAAIPLAWLGGDETFAKYALGPGDEMMALGFPRGLAANQAGFPILRSGR
                     VASYPLAPSTAFPTFLLDFSVFPGNSGGPVFMAQAARRRPGAAEAQEVQFIAGILTQQ
                     VELSGERLEIGIVTQARFIRETIDMLDGVAPPPRQPSEPPPAGSRTASADEAAEP"
     gene            3847..6021
                     /locus_tag="PLO65_03720"
     CDS             3847..6021
                     /locus_tag="PLO65_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD27390.1"
                     /translation="MNRRDLLAATAAAAVSSTITARSAMSATPKPVPPVARQEPRRIE
                     QLGRVRVDEYAWMKDDNWQQVLHDPKLIKAEVKAHLEAENAYFKAMLGSTEPLQATMF
                     EEMKGRIKEDDSSVPAPDGPWEYYTRYETGAQHPFYCRRPRGAADGEQILLDAEALAR
                     GKAFSEVGAAAHSPDHSLFAYAEDAQGSEVYRIYVKDLATGEILPGPVENTYADFTFS
                     PDSQWLFWTHRDENGRPDKIYRRPARGGAGSDVLVYHEQDEGFFIGVYTLSADTFIAI
                     GCGNQETSEIRLIPASDPTATPVVVEPRRVGVLYDLEQWGDELIIRTNAGDAVDFKLV
                     KAPVATPGAAHWQDFVAHEPGRMIEGVTAFKGHLVRLEKADALNRVVVLNRAGDETVL
                     RFDEEAYAVSLEGGYEYDTTTLRFVYQSPTTPKQWFDQDMATGQRTLRKTQEIPSGHN
                     PADYVTRRLNATAPDGQQVPIFVLMKKDAKLDGSAPLLLYGYGSYGASNEPTFNIRQF
                     SLVDRGWIWAVACVRGGAEKGRGWFLDGRKFRKKNTFTDFIACAEHLHAAGYGKPENT
                     VAFGRSAGGMLMGTITNMRPDLFAGIIAGVPFVDVLNTMSDPSLPLTPPEWPEWGNPL
                     TDPEAYDYIASYAAYEQVAAKPYPAVLAYAGLSDPRVTYWEPEKWTSRLRRHTTSSNP
                     VLLWVNMDAGHAGSAGRFDYLKEVARDLAFATWAVDRGWAKA"
     gene            6006..6563
                     /locus_tag="PLO65_03725"
     CDS             6006..6563
                     /locus_tag="PLO65_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRD27391.1"
                     /translation="MGEGLIVRAATAADAEALAAIYGDACLHGTGTFEETPPGADEMA
                     RRQAAVVARGLPYYVAEMDGRVAAYAYASPFRPRTGYRYTAEDSVYVAPWAKGRGLGG
                     LLLTRVIADCEQRGLRQLMAVIGGSENIASIALHRALGFELKGVMTGVGFKFGRWMDI
                     VMMMKPLNGGTETLPEGPGLPLGGG"
     gene            7069..8394
                     /locus_tag="PLO65_03730"
     CDS             7069..8394
                     /locus_tag="PLO65_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27392.1"
                     /translation="MTPTTRTRLFGATALAASLTLAPAAFAQDHSMHDMPGMATPTPP
                     AAAGYPDVEEVIVADSRIMLSPLGPYTANRDAGGTAWQPDATPHGGVHLMAGDWMVMV
                     HGELNAVYSDQSGPRGDSRSFAAGMLMGMASRDLGPGKLQLKAMVSPDPFMGPEGYPL
                     LLATGETADGVTHLVDRQHPHDLFMELSASYSLPVGDKGSLFVYGGLPGEPAFGPPAF
                     MHRAAAMDSPEAPISHHWFDSTHVTFGVVTGGFTWDRWKVEASAFRGREPDQNRWDIE
                     TGDLDSRSARISFNPTENWSLQASWADITSPEALEPLVDEERVSLSALYGRAFGSAGF
                     VSATGAWARKDKDGETLDAWLFESAIQFNDSWTLFARAEQVDQAELAPGNIYTVRKVS
                     LGAIHDWRVGDDIKLGVGALINGFDIPSPLAASYGDAGGGMAFVRLKIG"
     gene            8459..9547
                     /locus_tag="PLO65_03735"
     CDS             8459..9547
                     /locus_tag="PLO65_03735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27393.1"
                     /translation="MTSTRRGALGGALAVGLSSARPAWSGGVDPVKSVDAAVQRASAL
                     GFSGQVHLETAGKVVLSKAYGRIGPGDAPPVTRRTRINIASVSKSITATVVFCLIDTG
                     RLALTDRIDRFLPDAPADKAGITLAQLMEHSSGLPNSYAADGIARRDQAMTAILAEPL
                     AAAPGDGSLYSNLNYEVLAAIAEIASGETYADLTRHEVLAPAGMVDTRLWAELDPRSS
                     PDIARIAPTDPEAAFDVAALGPNWGYTGSGGVWSTADDIAAFVRAVMAGRIIRPASVE
                     AMLAPRTGTRGPSAFTYGWFVRGGRRPMIWTRGVEDWGHNAVAYWWPDARTLLTLTSN
                     AGQHEGLAWSRWLGESLEPVLFDRPGAA"
BASE COUNT         1564 a         3416 c         3370 g         1440 t
ORIGIN      
        1 tttctgtggc gctatccctc gggtcacccc gggcgggcgt tacccgccgc cttgtttccg
       61 tggagcccgg actttcctcg acccccgaag ggaccgcgat cgcccggccg tctgatccgt
      121 cgcggtatgt gggggaaggc ggaggtgggg tcaatgatgg tgcgtccttc gacacggccc
      181 ttcgggcctg ctcaggatga cgtgctttat tgggagcctt ctctccacgt catggtgagc
      241 agcgcgaagc gcgtgtcgaa ccacgcaaca tttatccgct ggcccgcagc accgccatca
      301 gccgcgcccg ggtcaggccg tcggccacgc cgtcggcgcg gctctggacc cagtgacgct
      361 ggaaggcggt gaccacctcg cgggtgcccg cgtcgaaccg gccggtgggg gcgcagtcga
      421 agccgagccg cgtcaggccg gcctgcaggg cgaacacgcc gacgccctcc gcgccctcgg
      481 ccagcggctc gccgggcgcc gggtcggtct cggcccagac gccatggccg gcctgcgcca
      541 gctgcttcca cggaaacagc tcccccggat cctgcttgcg cgcgggcgcg acgtccgaat
      601 ggccgaggat gcgatcgtcc tcgatgctcc agcggccgcg gatgtcggtg agcagggcga
      661 tcaccgccgc gatctgggcc tcgggaaacg gccggtagcc gaactcgtgg ccgggattga
      721 cgatctcgat gccgatcgag gcgtggttga ccccctcctc gcccttccag aacgaacggc
      781 cggcgtgcca ggcgcggcgc tcctcggcga ccagccggaa gatccgaccg tcctcctcga
      841 ccatgtagtg ggacgagacc cgggcctcgg gatcgcggag ccgggccagg gcctcggggc
      901 cgctcttcat gccggtgtag tgcagcacca gcatgtccgg gaccttggtc cgggcgtcga
      961 agttgggcga cggggcgtcg atgaggtcga gcgtcattgg aaccggtttc gcatcagcag
     1021 cagcaacggc atcacatggt tgggccgcag cgcgatgatc agcacgccgg ccagcgccag
     1081 ggtggcgccg atggccattc tggcatcgaa atggtcatgg gtgacaagca cgcccagcac
     1141 gatcgtgaac agcggcgtca gcagcgtcag cggcgagatc agcgtcgcct cgtatttctg
     1201 gatcaggccg tagtaggcgg tgtgggcgat caccgagatg acgatcgccg aatagagcac
     1261 cgccgccagc aacggccagc ccgcggccag ggtcgcggcc cactggccct gttcggtcag
     1321 ggcgctgaag ccgagcagcg gaatggccga ggtgaagccg acccaggcct ggaactgcag
     1381 cggcctgacc ccctccatct gtttcatcat caccgcgccc agcgcgcccg ccaaggccgc
     1441 cgccgcgacc agccaaaggc ccagcgacaa cgccatgccg ccgggacgcc acatcaccgc
     1501 gaccgccccg gcgagcgtca gggccatgcc gatcccgcgc cgccagcgca cctgttcgcc
     1561 gagcatgatc atcgacagga cggtggtcat cggcacgccc agctggatga cgatcgaggc
     1621 cgccgacggg ctggccgtct tcagggccat gaacatcagg gcgaagttgg cgccgccgat
     1681 cagctggcag acgatgatca tccgccagag cggccgcggg gccgggaaca gccatggcag
     1741 ggtgcagagc atgaccacca cgaaccgcgc gccggcatag tacagcggcg gcacgcccca
     1801 gtgggcgatg acgatcttgc tcacgatggt gttggtcgcc cagacgaaac acatcactac
     1861 cagaagggcg aagtcgcgca gggccatgac ggaccgatag aacattcggg cgcgtattgc
     1921 cgaacagatt gtcgcgccgg ttggcgcgaa gcggaaaaag tcggccgcgc ctcccctggt
     1981 cggccacgcc agggcggcgg gcgaatggcg atgcgcgcca cggatcgggc ttcccttcac
     2041 tgccggctca aggcagaaag cccccaacaa ctgtttgatg ggagctgcgc gtcatgaagg
     2101 aagtggactt caaggatctg gcgagcctgg ttggccagga gatcggcgtc tcggactggg
     2161 tccagatcac ccaggagcgg gtcaaccagt tcgccgaggc gaccggcgac catcagtgga
     2221 tccatgtcga cgtcgagcgg gccacccgcg agatgggcgg cccgatcgcc cacggctacc
     2281 tgacgctgtc gctgatcccg ttcctcggcg cgggcctgtt gcgggtcaag ggcgtgacgc
     2341 gcggcatcaa ctacggcagc gacaaggtgc gcttcaccaa catggtccgc gtcggcaaga
     2401 aggtgcgcat gcgccagaag ctgctcagcg tcgagccgaa ggccggcggc ctgcagatga
     2461 agaacgaatg cacgatcgag atcgagggcg aggagcgtcc ggcctgcgtc gccgagacca
     2521 tttcggtcat ctacggctag gagcgtcgcc cctgggcgac aggcgaaaat gacgacggcc
     2581 gcggatcgct ccgcggccgt tttcatgtct ggcggcgatc agctcctgaa ggagcgatct
     2641 tcatccatcc gccgctcgaa ggcgctgatt tcctcatccg ccttgtcctt ggcgtagccg
     2701 tagcgctcct ggatcttgcc ggccagctgg tcacgcttgc cggccaccac ggtgaggtca
     2761 tcgtccgtga gcttgcccca ctgttccttg accttgccgc tcatttcctt ccacgagcct
     2821 tcgatacgat cccacatggc tgtactccct gcatgacgat ggcgtccgta aacgccttgc
     2881 agaaacaacc gttgagggcc gtcagggttc cgccgcctcg tccgcagacg cggttctgga
     2941 gccggccggc gggggctccg acggctgtcg cggcggcggc gcgaccccgt cgagcatgtc
     3001 gatcgtctcg cggatgaatc gggcctgggt gacgatgccg atctccagcc gctcgcccga
     3061 gagctcgacc tgctgggtca ggataccggc gatgaactgg acctcctggg cctccgccgc
     3121 gccgggccgg cggcgggcgg cctgggccat gaacaccggc cccccggaat tgccggggaa
     3181 gacgctgaaa tcgagcagga aggtcgggaa ggccgtcgac ggcgccagcg gataggaggc
     3241 cacccggccc gaccgcagga tcggaaaccc ggcctggttg gcggccagtc cgcgcgggaa
     3301 gcccagggcc atcatctcgt cgccggggcc cagggcgtat ttggcgaagg tctcgtcccc
     3361 gcccagccag gccaatggaa tggctgcctg ggcgaactcg ggcggagcct tgatgacgat
     3421 cgccgcgaca tcgcggtcgg gatgcttggt ccacagcggc ttgccgtcgt cgcggatcgt
     3481 caggggctcg ggatcgtagc gccagccccc gtcggacgtc tgggcgcgaa agccgatcct
     3541 ggccgaggcg atcggcatgc ggtcgaacac atggccggcg gtgaccagca cgacgcgcgg
     3601 cttgccgtcg ggcgtcggcg cgttgaccag gaacccggtg ccgaccgtgc gcgtgccgtc
     3661 gcccagcggc tgttcgagct gcaccgtcgc cttgatcagg tcgacggaaa ggtcccaggc
     3721 catcgacgcc ccctgcctgt acgcggtact gcgggctgtt tcgcccgttc gatgatgcgc
     3781 gctcatttga ccagaaccgc gggcgcgcac aagctgtcgt cctgccgcac cttcgagagt
     3841 tgattcatga accggcgaga ccttctggcc gcgaccgcgg ccgccgcagt gtccagcacc
     3901 atcaccgccc ggagcgccat gtccgccacg cccaagcccg tcccgcccgt cgcccgccag
     3961 gagccccggc gcatcgagca gctcgggcgt gtccgggtcg acgaatacgc ctggatgaag
     4021 gacgacaact ggcagcaggt gctgcacgat cccaagctga tcaaggccga ggtcaaggcg
     4081 cacctcgagg cggagaacgc ctatttcaag gccatgctgg gttcgaccga gccgttgcag
     4141 gcgacgatgt tcgaggagat gaaggggcgc atcaaggagg acgactcctc cgtccccgcg
     4201 cccgacgggc cgtgggaata ctacacgcgc tacgagaccg gggcgcagca tccgttctat
     4261 tgccgccgac cgcgcggcgc ggccgacggc gagcagatcc tgctcgacgc cgaggccctg
     4321 gcccggggca aggccttttc cgaggtcggc gccgccgccc acagtcctga ccacagcctg
     4381 ttcgcctatg ccgaggacgc gcagggatcc gaagtctacc gcatttacgt caaggacctg
     4441 gccaccggcg agatcctgcc gggaccggtg gagaacacct acgccgactt caccttctcg
     4501 ccggactcgc agtggctctt ctggacccat cgcgacgaga acggccggcc ggacaagatc
     4561 taccggcggc cggcccgcgg cggggcgggc agcgacgtgc tggtctatca cgagcaggac
     4621 gaaggcttct tcatcggcgt ctacaccctg tcggccgaca ccttcatcgc catcggctgc
     4681 ggcaaccagg agaccagcga gatccggctg atcccggcgt ccgaccctac cgccacgccg
     4741 gtcgtcgtcg agccgcgcag ggtcggggtt ctctacgacc tggagcagtg gggcgatgag
     4801 ttgatcatcc gcaccaacgc cggcgatgcg gtcgacttca aactggtcaa ggctcccgtc
     4861 gccaccccgg gcgccgccca ctggcaggac ttcgtggcgc atgagccggg ccggatgatc
     4921 gagggcgtga ccgccttcaa gggccatctg gtcaggctcg agaaggccga cgccctcaac
     4981 cgggtggtgg tgctgaaccg cgccggcgac gagacggtgc tacggttcga cgaagaggcc
     5041 tacgccgtgt cgctggaggg cggctacgag tacgacacca ccaccctgcg cttcgtctac
     5101 cagtcgccga ccacgccgaa gcagtggttc gaccaggaca tggccaccgg tcagcggaca
     5161 ctgcgcaaga cccaggaaat tccgtccggg cacaatccgg ccgactatgt cacccggcgg
     5221 ctgaacgcaa ccgcgccgga cggacagcag gtcccgatct tcgtgctgat gaagaaggac
     5281 gcgaagctcg acggctccgc ccccctcctg ctctacggct acgggtccta cggcgcgtcg
     5341 aacgagccga ccttcaacat ccgccagttc agcctggtcg accggggctg gatctgggcc
     5401 gtggcctgcg tgcgcggcgg ggcggagaag gggcgcggct ggttcctcga cgggcgcaag
     5461 ttcaggaaga agaacacatt caccgacttc atcgcctgcg ccgagcatct gcacgccgcc
     5521 ggctacggca agccggagaa caccgtcgcc ttcggccggt cggccggcgg catgctgatg
     5581 ggcacgatca ccaacatgcg gccggacctg ttcgcgggga tcatcgccgg ggtgccgttc
     5641 gtcgatgtgc tgaacaccat gagcgatccc agcctgccgc tgacgccacc cgaatggccc
     5701 gagtggggca acccgctgac cgatccggag gcctatgact acatcgccag ctacgccgct
     5761 tatgaacagg tggcggccaa gccctacccg gccgtgctgg cctacgccgg actcagcgac
     5821 ccgcgggtga cctactggga gcccgaaaaa tggacatcgc gtctgcggcg gcacacgacc
     5881 agtagcaatc ccgtcctgct gtgggtgaac atggacgccg gacacgcggg ctcggccggg
     5941 cgcttcgact atctcaagga agtcgcccgc gacctggcat tcgcgacatg ggcggtggat
     6001 cggggatggg cgaaggcttg atcgtacggg cggcgacggc cgccgacgcc gaggccttgg
     6061 cggccatcta cggcgacgcc tgcctgcacg gcacggggac cttcgaggag acgccgccgg
     6121 gcgccgacga gatggcccgg cgccaggcgg cggttgtggc caggggcctg ccctactacg
     6181 tcgccgaaat ggacggccgg gtcgccgcct atgcctacgc ctcgccgttc cggccacgga
     6241 ccggctatcg ctacacggcc gaggacagcg tctatgtggc gccctgggcc aagggcaggg
     6301 gcctgggcgg cctgttgctg acgcgggtga tcgccgactg cgaacagcgg gggctgcgcc
     6361 agctgatggc cgtcatcggc ggctcggaga atatcgcctc gatcgccctg caccgggcct
     6421 tggggttcga gctgaagggt gtgatgaccg gcgtcggctt caagttcggc cgctggatgg
     6481 acatcgtgat gatgatgaag ccgctcaacg gcgggacgga gacccttccg gaagggccgg
     6541 ggctaccgct gggcggcggg tagggtcggg agaggttgcg tggttcgaca cgcggcttcg
     6601 ccgctgctca ccatgacgca gaggaaatgc acgtcatcct gagcgggccc gaagggccgt
     6661 gtcgaaggac gcgccgcgcg cattgtcatc gacccgccct ctccctaact tccgccccat
     6721 gcggagtctg cgcaccctcg tcctggtcct gatcgcggcg ttgttcgccg tcagcgggac
     6781 cgcgcgggcc atgccggcgc ccgccgggcc gcccccctgc catgaggccc cggcccagca
     6841 cggcaagtcg atgccggccc cggccgcgat gtcctgctgt gtcggctgca tgccggcccc
     6901 gacggaggtt ccgcccctgc tgggcgggcg gcctgccgag gctgcggtct atgcagacgc
     6961 ccgggatctt cgcacgggcc gggtgaccgc ccccgatccg gatccccctc gccttcgcgt
     7021 ctgacccctc gaaccgctac attcgttctc agacagaagg agggtccgat gacccccacg
     7081 acccgtacgc gcctgttcgg cgccacggcc ctcgccgcga gcctgacgct cgcccccgcc
     7141 gcgttcgccc aggaccacag catgcatgac atgccgggca tggcgacgcc gacaccgccg
     7201 gccgccgccg gctatcccga tgtcgaggag gtgatcgtcg ctgactcccg gatcatgctc
     7261 agcccgctcg gcccctatac cgccaatcgc gacgccggcg gcaccgcctg gcagcccgac
     7321 gccacgccgc acggcggcgt tcatctcatg gccggcgact ggatggtcat ggtccatggc
     7381 gagctcaacg ccgtctacag cgatcagtcc ggcccgcgcg gcgacagccg gtcgttcgcg
     7441 gccggcatgc tgatgggcat ggcctcgcgc gatctcgggc cgggcaagct tcagctcaag
     7501 gccatggtca gtcccgaccc gttcatgggt cccgagggct atcccctgct gctggccacc
     7561 ggcgagacgg ccgacggggt gacccatctg gtcgaccgcc agcacccgca cgacctgttc
     7621 atggagctgt cggccagcta cagcctgccg gtcggcgaca agggcagcct gttcgtctat
     7681 ggcggcctgc ccggcgagcc cgcgttcggc ccgccggcct tcatgcaccg cgcggcggcc
     7741 atggacagcc ccgaggcgcc gattagccac cactggttcg actcgaccca cgtgaccttc
     7801 ggcgtggtca ccggcggctt cacctgggac cgctggaagg tcgaggcctc ggcctttcgc
     7861 ggccgcgagc cagaccagaa ccgctgggac atcgagaccg gcgacctcga ctcgcggtcg
     7921 gcgcggatca gcttcaaccc gaccgaaaac tggtcgctgc aggccagctg ggccgacatc
     7981 accagcccgg aggcgctcga gccgctggtc gatgaggagc gggtgtcgct gagcgccctc
     8041 tatggccggg cgttcggcag cgccggcttc gtctcggcca ccggcgcctg ggcccgcaag
     8101 gacaaggacg gcgagacgct cgacgcctgg ctgttcgaga gcgccatcca gttcaacgac
     8161 agctggaccc tgttcgcgcg ggccgagcag gtcgaccagg ccgaactggc gcccgggaac
     8221 atctacaccg tgcgcaaggt ctcgctcggc gcgatccacg actggcgggt gggcgacgac
     8281 atcaagctgg gggtcggggc gctgatcaac ggcttcgaca tcccgtcgcc actggcggcc
     8341 agctatggcg acgccggcgg cgggatggcg ttcgtccggt tgaagatcgg atagccatca
     8401 tcccgccatc gagcgccctg cagaatggcc gccctgtccc tcccgtccga ggccttcaat
     8461 gacttcgacc cgcagaggcg ccctgggcgg cgcgcttgcc gtgggcctct cgtcggccag
     8521 accggcgtgg agcggcggcg tcgacccggt caagtcggtc gacgccgccg tccagcgggc
     8581 cagcgccctg gggttcagcg gccaggtcca cctggaaacg gccggcaagg tcgtcctgag
     8641 caaggcctat ggccgcatcg gccccggcga cgcgcctccg gtcacccgcc ggacgcggat
     8701 caatatcgcc tcggtcagca agtcgatcac cgccaccgtg gtgttctgcc tgatcgacac
     8761 cggacggctg gcgctgactg accggatcga tcgcttcctg ccggacgcgc cggcggacaa
     8821 ggccgggatc accctggccc agctgatgga gcacagttcg ggcctgccca acagctatgc
     8881 cgccgacggg atcgcccggc gagaccaggc catgaccgcg atcctcgccg agccgctggc
     8941 ggccgcgccc ggcgacggct cgctctactc caacctgaac tacgaggtgc tggccgccat
     9001 cgccgagatc gcgtcgggcg agacctacgc cgacctgacc cgccatgagg tgcttgcgcc
     9061 cgccggcatg gtcgacaccc ggctctgggc ggagctcgac ccccggagca gtcccgacat
     9121 cgcgcggatc gcgccgacag atcccgaggc cgcgttcgac gtcgcggccc tcgggccaaa
     9181 ctggggctac accggatcag gcggcgtctg gtcgacagcc gacgacatcg ccgccttcgt
     9241 tcgcgcggtg atggcggggc ggatcatccg gccggcgagc gtcgaagcca tgctggcgcc
     9301 gcgcacggga acgcgggggc catcggcctt cacctatggc tggttcgttc gcggcggccg
     9361 gcggccgatg atctggaccc ggggcgtcga ggactggggc cacaacgccg tggcctactg
     9421 gtggcccgac gcgcggaccc tgctgactct caccagcaac gcgggacagc acgaaggcct
     9481 ggcctggagc cgctggctgg gcgagtcgct ggagccggtg ctgttcgaca ggccgggcgc
     9541 ggcctgatcc ggtcgacccc cgccgagggc cgaggagtcg tgggacttcg ccagcgaaat
     9601 gtcgccctgg aagcggcggg cgcagcggtt gagggtcagc gggcggccga gcgccccgcg
     9661 gtcagccggg ccatgaagac ctcgtgcggc tcgagggagc gacccgcgcc gacgcggttg
     9721 gcgagggcgg ccgcgtcgtc gacccagcgt tcgatgtcct cccggacggc ccggtcgcgc
     9781 cagccgtggg
//
LOCUS       DAORNX010000052         9776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5130_length_9776_cov_6.805781, whole genome shotgun sequence.
ACCESSION   DAORNX010000052 DAORNX010000000
VERSION     DAORNX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_9776_cov_6.805781"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>272
                     /locus_tag="PLO65_03740"
     CDS             <1..>272
                     /locus_tag="PLO65_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27394.1"
                     /translation="IGPGALLTEGAISGSARVVDSAGGPRATMAFNARNAALRGANGL
                     RLSSAVITAEGPLERLPVKLEGRGLYGANPWRFKGGGFFAQRGGST"
     assembly_gap    273..382
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <383..2060
                     /locus_tag="PLO65_03745"
     CDS             <383..2060
                     /locus_tag="PLO65_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRD27395.1"
                     /translation="VEGGKAAITARLDGEAANIKASLTGVSLGALNEDLAGQFDGELT
                     LQGEGGRLTGALDATLRDARARGAGRAGSVTGDVKARLSDATLTIVGSATNAGGLRSG
                     LTVALPVEAAAAPLRLAIARQKPMRGEFMAEGEIKPLWDLLVGGERRVTGQVSMAGTI
                     GGTLADPRLAGRAELSHGVFEDTVVGLKLEDLSIVAALSDNAIDVSRFSGSDGRGGRV
                     SGGERGQLSGGGRISLQRDGVSTFRVNLKDFRLIENEAVTAIASGDTTINRAADGKVQ
                     VSGALTIDRATVAANPPTPSGVVPMAVVEINRPESAEVEVLAEPSRGLVIALDVTLKA
                     ARGIFVEGRGLDAELSLDAHVGGTTARPVLDGVARVVRGEYDFAGKRFEFDQRGAVYL
                     ATSPERIRLDLTATRDDPALTAVIRVRGTAAKPEITLTSTPVLPNDEVLSRILFGASA
                     SQLSPIEAAQLASALAALAGGGGFDVIGNLKNFAGLDRLVFAGGGAAGAMTVAGGKYL
                     TDDVYLEIIGGGRDGPAAQVEWRVRRNLSIISRLQGEGGSKLSVRWRRDD"
     gene            2375..3622
                     /locus_tag="PLO65_03750"
     CDS             2375..3622
                     /locus_tag="PLO65_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRD27396.1"
                     /translation="MRLSPEEKAVLDALGEQGSAIVDRAVAWCAVNSGSRNLAGLEAT
                     RAILKDAFAALPTEPQDMPLAPSIEIAADGQQREQQHPPAIAVIVRPEAPVQVVLTGH
                     YDTVYPAESRFQTVTTRPDGALHGPGIADMKGGISVILAALHAFEAHPDAGRVGYRVL
                     LSPDEEIGSLASAPVLAEFGRLGHVGMTYEPALADGTLAGERKGSGNFHIRVTGRTAH
                     AGRDFAIGRNAITAAARIAGALDALNGLRDGVTLNVARIDGGSPLNVVPDIAVVRFNV
                     RFPDAAAAAWLEAGIATALAAGQGDGLDASLHGGFTRPAKPFNAAQAALFGTVREVGG
                     LLGQDIAWKPSGGVCEGNNLFAVGLPNVDTLGVRGGDIHSENEFAWPDSFAERAQLSA
                     LILMKLASGEIDARGIHALMESR"
     gene            3622..4626
                     /locus_tag="PLO65_03755"
     CDS             3622..4626
                     /locus_tag="PLO65_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HRD27397.1"
                     /translation="MLVVRPAVPADYEALMELAVLSGRGFTSLPEDEPTLRARLDLSL
                     ASFSGAVAPPEAWYTLMLEDTETGAIDGVAGVKASVGLKRPFFSFRVVTLAQSSPTLE
                     MRFDHQALVLVNECAGWSEVGSLFLKPERRKGGAGRLLAQSRYMLIGIEPQRFAEMVL
                     AELRGWFDEDGSCPFWDDVASKFFNLPFDEADRMSASTNGQFILDLAPRHPIYSGLLP
                     EKARKSVGRVHRDGEAARAMLEREGFRYQGLVDLFDAGPTMGAPRDDIRTVKDARRLT
                     VRVGEDAFGEEALVSTHDVAKFRAVRTPVLVDGETAVLSREAADVLHVREGDLVKVKA
                     "
     gene            4841..6295
                     /gene="astD"
                     /locus_tag="PLO65_03760"
     CDS             4841..6295
                     /gene="astD"
                     /locus_tag="PLO65_03760"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HRD27398.1"
                     /translation="MSRGLYINGVWSEGHGPALTSIDPATGEPVWQAATAAAEDVARA
                     VAAARHAFQDWADQPREERIAVMRRYKAALDAGKDAFAEALSRETGKALWETRAELGS
                     MMAKVDVSIAAYEERTGVKETAMPFGRAMLRHRAHGVMAVLGPFNFPGHLPNGHIVPA
                     LLAGDTIVFKPSEETPMAGELLVEALDAAGLPPGVVNLVQGGRDAGAALIEQEIDGLL
                     FTGSASAGSHFRRVFADRPDVILALELGGNNPLVVWDTADAEAAAALIVQSAFITTGQ
                     RCSCARRLIVPEGHQGEAVIEAVVALSDRLIAGAWSSNPEPFMGPLISARAGRLARDA
                     AALLEGSVIRPARSIDGLSEAFITPGVIDVSHHETPDEEIFAPVLQVRRAATFDGAIA
                     LANATRYGLSAGLISDDPARWDRFLSRIRAGVVNWNRPTTGAAGSAPFGGLGASGNHR
                     PSAYYAADYCAYPVASFEADAVLPTVGDIKGLRA"
     gene            6420..7751
                     /gene="astB"
                     /locus_tag="PLO65_03765"
     CDS             6420..7751
                     /gene="astB"
                     /locus_tag="PLO65_03765"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HRD27399.1"
                     /translation="MTAVEANCDGLVGPTHSFAGLAPGNLASEHNKGDASNPRSAVLE
                     GLSKMRLMADLGLPQFVLPPHERPDVGFLRAIGFSGTDAAVIEAAGRQAPELLNTACS
                     ASPMWAANAATVTPSADAADGRVHFTPANLLTNLHRSLEGGQTTRALRRLFPDPARFA
                     VHDPLPAAPHFADEGAANHVRLCADHGEAGVNLFVWGREAWESWQGRFPARQTLQAFE
                     AVARRHGATGAVFARQARTAIEAGVFHNDVVCVGARTCLFYHEGAFEDAAALQRDIRA
                     AAGGRFEPQFVEVTQADLPLGDAIRSYLFNSMLVALPGEDRLTLVAPMETAETPRAKD
                     AVDRAIASNGPIGAVRYVDVRQSMRNGGGPACLRLRVALTDAELAAANPAQRFDATLH
                     GKLEAWANSHYRDRLRPADLADPQLLVESRRALDELTGILDLGSGFYPFQR"
     gene            complement(7790..8569)
                     /locus_tag="PLO65_03770"
     CDS             complement(7790..8569)
                     /locus_tag="PLO65_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRD27400.1"
                     /translation="MRAFAWGAGATAAIVFGVTLVNVATIRHDMSHLDWREPWIWEGS
                     SALAIYCLFWLPWLMQRVAPLEEWRSPRFWLIHAAATLVWSALHVAGFLLLRTGAYAL
                     AGDHYQYGDLRTEFPYELRKDVLSYALTSCAFWVARRLTLQSSAMTPDQPATFDIRDG
                     ARVTRVPITDILAVSSAGNYVEVRLADGRKPLMRSSLATVEGQLSPFGFVRTHRSWLV
                     SAARVTGLTPAGSGDYTVSLGSLEAPLSRRYPAALMRLRGG"
     gene            8716..>9776
                     /locus_tag="PLO65_03775"
     CDS             8716..>9776
                     /locus_tag="PLO65_03775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD27401.1"
                     /translation="MTPTSDRRPDLDWIRVGAFFLLILYHVGMFYVPWDWHVKSPQPV
                     EWLKPVMHLTSPWRLTLLFLVSGAATRFMADKMTAGALAGARTWRLLPPLIFAMLVIV
                     PPQSFYEIVEQAPQLAEPFWTFYGKYVTASGHWCDADGCLITPTWNHMWFVAYLFVYS
                     MILASVLTVARAPLIRLGDRLGAGLRGWRLLLLPIIFLAACKLGLEPIFGSTHALVDD
                     WYVHAISFTTFLFGFLTARSDSFRAATIRLRWPALLLAFSCWALIANYEWRHRADDMG
                     APLALQLLGGVIYAANQWGAIVAVLGFGAKHITRGGPLLTYLTLGVFPFYIVHQTVIV
                     VAGHHLAKLGLPQGLEAGIL"
BASE COUNT         1373 a         3472 c         3357 g         1464 t
ORIGIN      
        1 gatcggcccg ggagcgctcc tcaccgaggg cgcgatcagc ggctccgcgc gcgttgtcga
       61 cagcgccggc ggcccccgcg cgaccatggc cttcaacgcc cgtaacgcgg ccctgcgcgg
      121 agccaacggc ctgcggctgt catcggccgt gatcaccgcc gagggaccgc ttgagcgcct
      181 gccggtcaag ctcgagggcc gcggtctcta cggggccaat ccctggcgct tcaagggcgg
      241 cggcttcttc gcccagcggg gcggctccac ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nncgtcgagg gcggcaaggc ggcgatcacc gcgcgcctcg
      421 acggcgaggc ggccaatatc aaggccagcc tgaccggcgt gtccctcggc gcgctcaacg
      481 aagatctcgc cggacagttt gacggcgagc tgacgctgca gggcgagggc ggtcgcctga
      541 ccggcgccct cgacgcgacc ctgcgcgacg cccgggcccg cggtgcgggc agggccggct
      601 cggtgactgg cgacgtcaag gcccggctca gcgatgcgac cctgacgatc gtcggctcgg
      661 ccaccaacgc cgggggcctc cgctcggggc tcactgtggc gctgcccgta gaggcggcgg
      721 ccgcgcccct gcgcctggcc atagcgcgcc agaagccgat gcgcggcgag ttcatggccg
      781 agggcgaaat caagccgctg tgggacctgc tggtcggcgg cgagcggcgg gtgaccggtc
      841 aggtgtccat ggccggcacg atcggcggga ccctggccga tccgcgactg gccggccgcg
      901 cagagctgag ccacggcgtg ttcgaggaca cggtcgtcgg cctcaagctc gaggacctgt
      961 cgatcgtcgc ggccctgtcg gacaacgcca tcgacgtcag ccgcttctcg ggcagcgacg
     1021 gccggggcgg gcgcgtcagc ggcggcgagc ggggccagct gtccggcggg ggccgtatca
     1081 gcctgcagcg ggacggcgtc agcaccttcc gcgtcaatct gaaggacttc aggctgatcg
     1141 agaacgaggc ggtgaccgcg atcgcctcgg gcgacaccac catcaaccgc gccgccgacg
     1201 gcaaggtcca ggtctccggc gccctgacga tcgatcgcgc gacggtcgcc gccaacccgc
     1261 ccacgcccag cggcgtggtg ccgatggcgg tcgtcgagat caatcggcct gaaagcgcgg
     1321 aagtcgaggt gctggccgag ccctcgcgtg gcctggtgat cgctctggac gtcaccctca
     1381 aggccgcccg gggcatcttc gtcgagggcc gcggcctgga cgccgagttg tcgctcgacg
     1441 ctcatgtcgg cggcaccacg gcgcggccgg tgctcgatgg cgtcgcgcgg gtcgtccgcg
     1501 gcgaatacga cttcgccggc aagcggttcg agttcgatca gcgcggcgcc gtctacctcg
     1561 cgaccagccc ggagcggatt cgcctcgatc tcaccgctac ccgcgacgac ccggccctta
     1621 ccgcggtgat tcgtgtccgc ggcacggccg ccaagccgga gatcaccctg acctcgacac
     1681 cggtcctgcc caacgacgag gttctgtctc gcatcctgtt cggcgcgtcc gcctcgcagc
     1741 tgtcgccgat cgaggccgcc cagttggcct cggccctcgc ggccctcgcc ggcggcggcg
     1801 gcttcgacgt gatcggcaac ctgaagaatt tcgccggcct cgaccggctg gtgttcgccg
     1861 gcggtggcgc ggccggggcg atgacggtgg ccggcggcaa atacctgacc gacgacgtct
     1921 atctcgagat catcggcggc ggtcgcgacg ggccggcggc ccaggtcgaa tggcgggtcc
     1981 ggcgcaacct gtcgatcatc tcccgcctgc agggcgaggg cggcagcaag ctgtcggtgc
     2041 gctggcggcg ggacgactaa cggaccgatc ccttcactgt catcccgggc ggcgcggagg
     2101 ggcgacccgg gaccccgaca atttcccagc gatctgcatg aacccgaatc cgggtcccgg
     2161 ctcttcgttg cgcttggccg ggatgacagt gaatgtggtg ggccaggggc aggtgacgtc
     2221 gacggccgca gggggttcac cttccgctac ggcgcggcta tcaaggggca tcggcgatcc
     2281 ccgtcttgcg ccattccggc gggtcgctca tccgaaacac gcgtcgacat tcgtccgcgg
     2341 caatggcgtc ggtaaagctg gattgacccc gccgatgcgt ctgtcaccgg aagagaaggc
     2401 tgttctcgac gccctcggcg aacaggggtc ggccatcgtc gaccgggccg tcgcctggtg
     2461 cgcggtcaat tccggcagcc gcaatctggc cgggctcgag gcgacacggg cgattctcaa
     2521 ggacgccttc gcggcgctgc ccaccgagcc gcaggacatg ccgctggcgc cctcgatcga
     2581 aatcgcggcc gacggtcagc aacgggagca gcagcacccg cccgccatcg ccgtcatcgt
     2641 ccgccccgag gcgccggtcc aggtcgtgct gaccggccac tacgacaccg tctatccggc
     2701 cgagagccgc ttccagaccg tcaccacgcg gcccgacggg gcgctgcacg gccccggcat
     2761 cgccgacatg aagggcggca tcagcgtgat cctcgccgcg ctgcacgcct tcgaggccca
     2821 tccggacgcg ggcagggtcg gctatcgcgt gctgctgtcg cccgacgagg agatcggctc
     2881 gctggcctcc gcgcccgtgc tggccgagtt cggtcgcctg ggccatgtcg gcatgaccta
     2941 cgagcccgcc ctggccgacg ggaccctggc cggcgagcgc aagggctcgg gcaatttcca
     3001 catccgcgtc acgggccgga ccgcccacgc cgggcgcgac ttcgccatcg gccgcaacgc
     3061 catcaccgcc gccgcccgca tcgccggggc gctcgacgcc ctcaacggtc tgcgcgacgg
     3121 cgtgaccctg aacgtggcca ggatcgacgg cggttcgccg ctgaacgtcg tgcccgacat
     3181 cgccgtggtc cgcttcaatg tgcggttccc cgacgccgcg gccgccgcct ggctcgaagc
     3241 cggcatcgcc acggccctgg ctgccgggca gggcgatggc ctcgacgcca gcctgcacgg
     3301 cggcttcacc cgaccggcca agccgttcaa cgcggcccag gccgccctgt tcggcacggt
     3361 cagggaggtt ggtggcctgc tcggccagga catcgcctgg aagccctcgg gcggcgtctg
     3421 cgagggcaac aacctgttcg cggtcggcct gcccaacgtc gacacgctcg gcgtgcgcgg
     3481 cggcgacatt cacagcgaga acgagttcgc ctggcctgac agcttcgccg agcgggcgca
     3541 gctgtcggcg ctgatcctca tgaagctggc gtcgggagaa atcgacgccc ggggcattca
     3601 cgccctgatg gagtcccgtt gatgctggtt gtccgtcccg ctgtccccgc cgactatgag
     3661 gcgctgatgg agctggccgt cctgtcgggc cgcggcttca ccagcctgcc cgaggacgag
     3721 ccgaccctgc gcgcccggct ggacctgtcg ctggccagct tctcgggcgc ggtcgccccg
     3781 ccggaggcct ggtacaccct gatgctcgag gacaccgaga cgggcgccat cgacggcgtc
     3841 gccggggtca aggccagcgt cgggctcaag cggccgttct tctcgttccg ggtcgtcacc
     3901 ctggcccagt cgtcgccgac gctggagatg cgcttcgacc atcaggccct ggtgctggtc
     3961 aacgaatgcg ccggctggtc ggaggtcggg tcgctgtttc tcaagccgga acggcgcaag
     4021 ggcggcgccg gacggctgct ggcccagtcg cgctacatgc tgatcggcat cgaaccccag
     4081 cggttcgccg agatggtcct ggccgagctg cgcggctggt tcgacgagga cggcagctgc
     4141 ccgttctggg acgacgtggc cagcaagttc ttcaacctgc cgttcgacga ggccgaccgg
     4201 atgagcgcct cgaccaatgg ccagttcatc ctcgatctgg cgccgcggca tccgatctat
     4261 tcgggcctgc tgccagaaaa ggcccgcaag tccgtcggcc gcgtccatcg cgacggcgag
     4321 gcggcccgcg ccatgctcga gcgcgagggc ttccgctacc agggactggt cgacctgttt
     4381 gacgccggcc cgacgatggg cgcgccgcgc gacgacatcc gcaccgtcaa ggacgcccga
     4441 cgcctgacgg tcagggtcgg cgaggacgcc tttggcgagg aagcgctggt ttcgacccac
     4501 gatgtggcga agttccgcgc cgtgcggacg ccggtcctgg tcgacggtga gacggcggtg
     4561 ctgtcgcgcg aagcggctga cgttctgcat gtgcgcgaag gcgatctggt gaaggtgaag
     4621 gcatgagcca ggcgttcgcc ttcctccccc tcctgggcga gggggaccat gcgaagcgtg
     4681 gcggaggggg aagcgccgct cgtggacctc ttgggcgcca tcccttcgtc cgcccgatcg
     4741 gccagctcct ccgcctccca ctccaccacc ctgcgggtgg tccccctctc ccaggaggga
     4801 gaggcaagtc cttcctggcg tcgatttccg gagcgaaccc atgagccgtg gcctctacat
     4861 caacggtgtc tggtccgaag gccacggtcc cgccctgacc tcgatcgatc cggcgaccgg
     4921 cgagccggtc tggcaggcgg cgaccgccgc ggccgaggac gtcgcccgtg cggtcgcggc
     4981 cgcccgccac gccttccagg attgggccga ccagccccgc gaggagcgga tcgccgtcat
     5041 gcgtcgctac aaggccgcgc tcgacgccgg caaggacgct ttcgccgagg ccctcagccg
     5101 cgagaccggc aaggccctgt gggagaccag ggccgagctg ggctcgatga tggccaaggt
     5161 cgacgtctcc atcgccgcct acgaggagcg gaccggagtc aaggaaacgg ccatgccctt
     5221 cggccgcgcc atgctgcgcc accgggccca cggggtgatg gcggtgctcg ggccgttcaa
     5281 cttccccggc cacctgccca acggccatat cgttccggcc ctgctggccg gcgacaccat
     5341 cgtcttcaag ccgtcggagg aaaccccgat ggccggcgag ctgctggtcg aggcgctgga
     5401 cgcggccggc ctgccgccgg gcgtggtcaa cctcgtccag ggcggtcggg acgccggcgc
     5461 ggccctcatc gagcaggaaa tcgacggcct gctgttcacc ggctcggcct cggccggcag
     5521 ccatttccgc cgggtgttcg ccgaccggcc ggacgtgatc ctggcgcttg agctgggcgg
     5581 caacaatccg ctggtggtct gggacacagc agacgcggag gccgccgcgg ccctgatcgt
     5641 ccagtcggcc ttcatcacca ctggccagcg ctgttcctgc gcccggcggc tgatcgtgcc
     5701 tgaaggccat cagggcgagg cggttatcga agcggtcgtg gccctctccg accgtctgat
     5761 cgccggcgcc tggagcagca acccggagcc cttcatgggc ccgctgatct ccgcccgcgc
     5821 cggccgcctg gcccgcgacg cggccgccct gctggagggg tctgtgatcc ggccggcccg
     5881 gtcgatcgac ggcctgtccg aggccttcat caccccgggc gtcatcgatg tcagccacca
     5941 tgagacaccg gacgaggaga ttttcgcgcc agtcctgcag gtgcgccgcg cggcgacgtt
     6001 cgacggcgcc atcgccctgg ccaacgccac ccgctacggc ctgtcggccg gtctgatcag
     6061 cgacgatccg gcccgctggg accgcttcct cagccgcatc cgcgccgggg tggtcaactg
     6121 gaaccgcccg accaccggcg cggccggctc ggccccgttc ggcggcctcg gcgcctccgg
     6181 caaccaccgc cccagcgcct attacgctgc cgactactgc gcctatccgg tcgccagctt
     6241 cgaggccgac gcggtgttgc cgaccgtcgg ggacatcaag ggcctgcggg cgtgagctgc
     6301 gctgtcgtgc gtccttcgac acggcccttc gggcttgatc aggatgacgt gcatatagtc
     6361 cacgtcatgg tgagcagcgc caaaggcgcg tgtcgaacca cgcaagctgg atcagagcga
     6421 tgaccgccgt cgaagccaat tgcgacgggc tggtcgggcc gacccacagt ttcgccgggc
     6481 tggcgccggg caacctcgcc agcgaacaca acaagggcga tgcctccaac ccgcgctcgg
     6541 cggtgctgga ggggctttcg aagatgcggc tgatggccga tctgggcctg ccgcagttcg
     6601 tcctgccgcc gcatgagcgg ccggatgtcg gcttcctgcg ggccattggc ttttcgggaa
     6661 ccgacgcagc cgtgatcgag gcggcgggcc ggcaggcgcc ggagttgctc aacaccgcct
     6721 gctcggcctc gccgatgtgg gccgccaacg ccgcgacggt gacgccgagc gccgacgccg
     6781 ccgacggccg ggtgcacttc acgccggcca acctgctgac caacctgcac cgcagccttg
     6841 agggcgggca aacgacgcgc gccctgcgcc ggctgtttcc ggatccggcg cgattcgccg
     6901 tccatgatcc gctgcccgcc gcgccgcact tcgctgacga gggggccgcc aaccatgtcc
     6961 ggctgtgcgc cgatcacggc gaggccgggg tcaatctgtt cgtctggggt cgcgaagcct
     7021 gggaaagctg gcagggccgc ttccccgccc gccagacgct gcaggccttc gaagccgtcg
     7081 cccgtcggca cggcgcgacc ggcgcggtgt tcgcccgcca ggcccgcacg gcgatcgagg
     7141 ccggggtgtt ccacaacgac gtggtctgcg tcggggccag aacctgcctg ttctaccatg
     7201 agggcgcctt cgaggacgcc gcggcgctgc agcgggacat ccgggcggcc gcgggcggac
     7261 ggttcgagcc gcaattcgtc gaggtgaccc aggccgacct gccgctcggc gacgccatcc
     7321 gcagctacct gttcaattcc atgctcgtcg ccctgcccgg cgaggaccgt ctgaccttgg
     7381 tcgcaccgat ggagacggcc gagacgccgc gcgcgaagga tgccgtcgac cgggccatcg
     7441 cctccaacgg tccgatcggc gcggtgcgct acgtcgatgt gcgccagagc atgcgcaacg
     7501 gcggcgggcc tgcctgcctg cgcctgcggg ttgcgctcac cgacgccgag ctggcggcgg
     7561 ccaacccggc ccagcggttc gacgcgaccc tgcacgggaa gctggaggcc tgggccaaca
     7621 gccactaccg cgaccgtctg cgccccgccg acctcgccga cccgcagttg ctggtcgaaa
     7681 gccgccgcgc cctggacgag ctgaccggga tcctcgacct gggctcgggg ttctatccgt
     7741 tccagcgctg acggggacac gtaccgaagg cggtacatgt ccgaagggtt cagccgcccc
     7801 gcaaccgcat cagcgccgca gggtagcgcc gcgaaagggg cgcttccagc gatcccagag
     7861 acacggtgta gtcgcccgag cctgcgggcg tcagtccggt cacccgcgcg gcgctgacca
     7921 gccatgagcg atgggtgcgg acaaagccga agggcgacag ctggccctcg acggtcgcca
     7981 gggaggaccg catcagcggt ttgcgcccgt cggccaggcg cacctcgaca tagttgccgg
     8041 ccgacgacac cgcgagaatg tcggtgatgg ggacccgggt caccctggcg ccatcgcgga
     8101 tgtcgaaggt cgccggctgg tcaggggtca tggccgagga ttgcagggtc agtcgccggg
     8161 cgacccagaa ggcgcaggag gtcagcgcat agctgagcac atccttgcgc agttcatagg
     8221 gaaactcggt ccgcagatcg ccgtactgat agtggtcgcc ggccagagca taggcgccgg
     8281 tgcgcaggag cagaaagccg gccacatgca gggctgacca gaccagcgtg gcggccgcgt
     8341 ggatcagcca gaaccgggga ctgcgccatt cctccagcgg cgcgacccgc tgcatcagcc
     8401 agggcagcca gaacaggcag tagatcgcca gcgcgctgct gccttcccag atccacggtt
     8461 cgcgccagtc gaggtggctc atgtcgtggc ggatggtggc gacattgacc agggtgacgc
     8521 cgaacacgat ggccgccgtt gcgccggcgc cccaggcgaa ggcccgcatc tcggtcccgc
     8581 tcgtcccgga tgacacgccg ttcgtcacag gccgctcccg cttgtcacgc cggtcgcgcc
     8641 ggacgtggcc ttgccgtagc gggccccggg gccgctctgg aatgtgcggc taccgaccaa
     8701 tcgcgagaac atgccgtgac cccgacctcc gaccgccgcc ccgacctcga ctggatccgc
     8761 gtgggcgcct tcttcctgct gatcctctac cacgtcggga tgttctatgt gccctgggac
     8821 tggcacgtta agtcgcccca gcctgtcgag tggctgaagc cggtcatgca cctgaccagt
     8881 ccctggcggc tgaccctgct gttcctggtc tccggcgcgg cgacgcggtt catggccgac
     8941 aagatgaccg ccggcgccct ggccggcgcg cggacctggc gcctgctgcc gcctctgatc
     9001 ttcgccatgc tggtgatcgt gccgccgcag agcttctacg agatcgtcga gcaggcgccg
     9061 cagctggccg agccgttctg gaccttctac ggcaaatacg tgaccgcttc cggccactgg
     9121 tgcgacgccg acggctgcct gatcacgccc acctggaacc acatgtggtt cgtcgcctac
     9181 ctgtttgtct attcgatgat tttggccagc gtcctgaccg tcgcgcgggc cccgctgatc
     9241 cgcctaggcg atcgcctggg cgcgggcctg cggggctgga ggttgctgct cctgccgatc
     9301 atcttcctgg cggcgtgcaa gctggggctc gagccgatct tcggcagcac ccacgccctg
     9361 gtcgatgact ggtatgttca tgccatcagc ttcacgacct tcctgttcgg cttcctgacc
     9421 gcccggtcgg acagcttccg tgcggcgacg atccgtctgc gctggccggc gctgctcctg
     9481 gccttctcct gctgggcgct gatcgccaac tacgagtggc ggcatcgggc cgacgacatg
     9541 ggcgcgcccc tggcgcttca actgcttggc ggagtcatct acgccgccaa ccagtggggc
     9601 gcgatcgtcg ccgtgctcgg gttcggggcg aagcacatca cgcggggcgg gccgctgctg
     9661 acctatctga ccctgggcgt gtttcccttc tacatcgtcc accagaccgt catcgtcgtg
     9721 gccggtcatc acctggcgaa gctgggcctg ccgcaggggc tggaggcagg catcct
//
LOCUS       DAORNX010000053         9756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5141_length_9756_cov_7.222657, whole genome shotgun sequence.
ACCESSION   DAORNX010000053 DAORNX010000000
VERSION     DAORNX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_9756_cov_7.222657"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLO65_03780"
     CDS             <1..871
                     /locus_tag="PLO65_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD27402.1"
                     /translation="PSRGRVSNAPAGEGWGGLSVQHAVTRSVRDSATLLDIACQPQPG
                     DPYWQAPPATPFAAEVGRDPGRLRIAFSTEALTWGTMDPACATAVREAAALCDSLGHH
                     VEEARPDLDYLAMAEAVNMLVSPAIATTLQLEAQRRGRAIEPGEVETLSWLIAENGGK
                     ATAVEMAIAQQKLNVFARGMAAFFLKYDVLILSTLGSPPVPLGHMDTNAEDLSDYAEK
                     LYGFMPNTQLFNAGGQPAMSVPLGMSEDGLPIGVMFAAATGDEATLFRLAGQLETAAP
                     WAARRPAPGRASG"
     gene            868..2250
                     /locus_tag="PLO65_03785"
     CDS             868..2250
                     /locus_tag="PLO65_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27403.1"
                     /translation="MTRGPTAPLAELPLLQLVIYALPSVTLQFLFVPMTALLPAFYAR
                     ELSMSLATVGGVLLASRMADLVLDPLIGRLSDGTRTRWGRRKPWMVAGTPVLMLGAVL
                     LFMPLSLGLAPAWYLLVASMVIYLGGSMLGLAYSAWGAEVVSSYHGRSKVAGMREALG
                     VLGIVLAASVPAVTALYGHAVDRFTMGVLGWMILIITPVTVGLAIRFVPEPAILERAA
                     INWRQQLLGLWRNKPFRVLCIAFVVMNLGSSVATSTLVFFIVHYLRQPEVIGPVLLGS
                     FASVLAFVPVWVWISRRIGKHRAAGISLIAAILINAVVALLLQPGDGWWFVGAMIVAG
                     AASAGYLTLPLGMMGDIIDFDALRTGQNRGGLYFGIWSFAQKISPAIAVGVTLPLLTY
                     VGFDPASKDGTAGVTALKYIFALGSTPLFLIGALLLMAFPIDARRHAIIRRRLDSLAE
                     RNARDDGAEE"
     gene            complement(2254..2469)
                     /locus_tag="PLO65_03790"
     CDS             complement(2254..2469)
                     /locus_tag="PLO65_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27404.1"
                     /translation="MADGGEAREPPARLPIVSATTAALSAALFVAATALLVGPFAGLS
                     DWSGDGRAGKIRRLESGFSDLVDDLLR"
     gene            2628..3614
                     /locus_tag="PLO65_03795"
     CDS             2628..3614
                     /locus_tag="PLO65_03795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HRD27405.1"
                     /translation="MTFKALLASRIDRTVSVDLVEMTTDQLMDGDVSIDVDYSTVNYK
                     DGLALAGRPGVIQTYPLVPGIDLAGTVTASTHAGYVPGDRVVLNGWGLSQTHHGGFAQ
                     KARVSGDWLVKLPQAISTRDAMAIGTAGYTAMLAVLALEHAGVTPDRGPVLVTGAGGG
                     VGSVAILILSRLGYRVIASTGRPEESPYLTALGAAEIIDRKALSEPGPPIASPRWIGA
                     VDSVGSQTLANVLAQTDYRGAVAACGLAQGVDLPGSVFPFILRNVTLAGIDSVNAPFA
                     VRARAWDRLARDLDLSRLATTVTEVGLAEVPAVGRAILEGRVRGRTVVDVNA"
     gene            complement(3663..4436)
                     /locus_tag="PLO65_03800"
     CDS             complement(3663..4436)
                     /locus_tag="PLO65_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD27406.1"
                     /translation="MTYPIPNVSTDLTGQVALVTGASSGLGVRFARTLARCGAKVALT
                     ARRLDRLEALAAEIRAEGGEALALELDVTDADQLLAVVGKAEAAFGTVTILVNNAGIP
                     DAQRATKMSVDLIDRVIGVNLRAPYILSCEVARRLMAAKSPGRIVNIASVAAYEYRGN
                     GAALYAVTKSAVVRMTEALSVEWASANINVNAIAPGAFASEMMDGMLSRVGDISQHFP
                     RKRLGDPAQLDSTLLFLVSPASDFVTGACIRVDDGQSGR"
     gene            complement(4460..5515)
                     /locus_tag="PLO65_03805"
     CDS             complement(4460..5515)
                     /locus_tag="PLO65_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD27407.1"
                     /translation="MTATAPPVRDVTAAHRFDPAPLEAWLRRQVEDFGEAMIVQQFQG
                     GASNPTFLLTTRTAAGPRRYVLRKKPPGVLLASAHQVDREYRVMKALDGLVPVPKMRA
                     LCTDETVIGTAFYVMDYLEGRIFRDATLPGLEPAERAAIYDDLNATLAKLHKVDFAAA
                     GLGDFGKAGGYFERQVSRWTKQYRGAESELIPEMEALIAQLPRRIPKDDPVSIAHGDY
                     RLENVMFHPTEPRLIAVLDWELSTIGNPLADIAYNAFLWRSHSPSWGTLQGVDFAASG
                     IPTEAEYVAAYCRRMGRGPIEDWPFYMAFSIFRLASISQGVYRRVLAGNAASDRPAEN
                     GTPHLARQAIEILEGRD"
     gene            complement(5727..>7004)
                     /locus_tag="PLO65_03810"
     CDS             complement(5727..>7004)
                     /locus_tag="PLO65_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD27408.1"
                     /translation="NALGVPIAAGVLYPFFGVLLSPMIAAAAIAMPAQAAETGADAAE
                     IAQLRAQIQALSDRLAAQEAAQARADAQIQTIPAQVQTAVAAVPPPAPAPAKPSWTDK
                     TVVSGRMYFDLTNITQESDGSRVQPTGTGFDIKRFYIGVDHKFNDMFSGNVTTDFQYS
                     SAISSTELYIKKAYLQAKFSDALVVRLGSTDLPWVPFAEDMYGYRYVENTLIDRTKFG
                     TSADWGVHASGKLAGGMVSYAVAVVDGAGYKAPLRSKGMDVEGRISITPVEHLTLAVG
                     GYTGKLGKSKQGVTTFHTANRFDAMAAYTTSQFRVGVEYFKAEDWNNVSTVTSDSAEG
                     YSVFGSYQFTPTIGAFARYDRVEPNQDTAPNRQDDYFNVGVTYSPTKIVDLSLVYKRD
                     AVDKGFVSTSNGTIGGLNDGTYDEFGLFGQFRF"
     assembly_gap    7005..7170
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7171..7677)
                     /locus_tag="PLO65_03815"
     CDS             complement(<7171..7677)
                     /locus_tag="PLO65_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27409.1"
                     /translation="MRTAVIQIGPGHPPLERQLARLEALSPERYHVEETMTARSAQRI
                     LECLERQAEGDEVCFLSLATLRLGVGETVRLLKALTDRGVACLTLDDDDQVVRVDGQA
                     EAGPVLHLLSDLQARLDGDGAASPARPPEGARGLLGEAEIADIRRLARSGLSPRRIGL
                     IYRRTPDCI"
     gene            7836..8843
                     /locus_tag="PLO65_03820"
     CDS             7836..8843
                     /locus_tag="PLO65_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRD27410.1"
                     /translation="MPPLSTSTPAARYGATLALVGLGFAGRLALAPLFGDSHTFTVFY
                     PVVILAAYFFGAGPATLSAVVSAGLAYWFFAAPAQAWKGDVANLTTTLLFAFTATVSI
                     YFITGMARALDQLAAARARAEALARSHAELFGELNERVTNHLQLVAALLQLQARDETD
                     QSLSKALAEASARTILISRVHRTVAGEADKTLDFDVFARQLLDATLAARGHPPVRVEI
                     EEQGVRLPLDQATSVAIVLLECFNARLQLNTPALMRVRLRETDREATLEVAEARPRGE
                     DLPELDLRRQSIEAMVEQLGGRFSHRSDDDGALSALVFPRPGAGAAPMAVGPAAAPTL
                     H"
     gene            8928..>9756
                     /locus_tag="PLO65_03825"
     CDS             8928..>9756
                     /locus_tag="PLO65_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD27411.1"
                     /translation="MTDRPPPFSALRAVEAASRHRSFTWAAKELRITHSAVSQSIRRL
                     EAELGTPLFQRKGGAMEPSEAALRLAESYHDAARSLDRTLREITGGSRAGVLTVAMPA
                     DFGRLWFGAKLARLAEAMPDLRIEVRTTGSRDQDPDSDVVIDLATTEGREADVVGPEL
                     SCFPVCSPDLLSARRLDTPRQIVRAPLIAGGGCDWDGWTEHFAVASRPPAFAFDDAGM
                     ALDATARGAGVALTHLFAAEGHLDDGRLVALPFAVPTGRHLLLSAGPGQADLAARFAM
                     "
BASE COUNT         1374 a         3355 c         3371 g         1490 t
ORIGIN      
        1 gccatcccgc ggacgggtct cgaatgcgcc cgcgggcgaa ggctggggcg gcctgagcgt
       61 gcagcacgcc gtcacccgct cggtccgcga cagcgccacc ttgctcgaca tcgcctgcca
      121 gccgcagccg ggcgacccct actggcaggc gccgcccgcc acgccgttcg ccgccgaggt
      181 cggccgtgac ccgggtcgat tgcgcatcgc gttttccacc gaggccctga cctgggggac
      241 gatggacccg gcctgcgcca ccgccgtccg cgaggcggcc gccctgtgcg actccctcgg
      301 acaccatgtg gaggaggcca ggccggacct cgactacctg gcgatggccg aggcggtgaa
      361 catgctggtc tcgccggcca tcgccacgac cctgcagctc gaggcccagc ggcgcggccg
      421 ggcgatcgaa ccgggcgagg tggagacgct cagctggctg atcgccgaga acggcggcaa
      481 ggcgacggcg gtcgaaatgg ccatcgccca gcagaagctg aatgtgttcg cgcgcgggat
      541 ggccgcgttc ttcctcaagt acgatgtcct gatcctgtcg acgctcggca gtccgccggt
      601 cccgctcggc cacatggaca ccaatgccga ggacctcagc gactacgccg agaagctcta
      661 cggcttcatg cccaacaccc agctgttcaa tgcgggcggc cagccggcca tgtccgtgcc
      721 gctgggcatg agcgaggacg gattgccgat cggggtgatg ttcgcggccg cgaccggcga
      781 cgaggcgacc cttttccgcc tcgccgggca gctggaaacg gcggcgccct gggcggcaag
      841 acgtcccgcc ccgggccggg cgtccgggtg acgcgcggcc ccaccgcccc gctggcggaa
      901 ctcccgctcc tgcaactggt gatctacgcc ctgccctcgg tcacgctgca gttcctgttc
      961 gtgcctatga ccgccctgct cccggccttc tacgcccggg aactgagcat gagcctggcg
     1021 accgtcggcg gcgtgctgtt ggcctcgcgc atggcggacc tggttctgga tcccctgatc
     1081 ggccgcctgt cggacggaac ccggacccgc tggggccgac gaaagccctg gatggtcgcc
     1141 ggcacgccgg tcctgatgct gggggccgtc ctgctgttca tgccgctgag cctcggcctg
     1201 gcgcctgcct ggtacctgct cgtcgcctcc atggtgatct acctgggcgg ctcgatgctc
     1261 ggcctggcct attccgcctg gggggcggaa gtcgtgtcca gctatcacgg ccggtcaaag
     1321 gtcgccggca tgcgcgaggc gctcggggtg ctgggcattg tgctggccgc cagcgtgccg
     1381 gccgtcaccg ccctctacgg ccacgccgtc gaccgcttca cgatgggcgt gctgggctgg
     1441 atgatcctca tcatcacccc cgtgaccgta ggcctggcga tccgcttcgt gccggaaccg
     1501 gcaatcctcg agcgggcggc catcaactgg cgccagcagc tgctcggact ctggcgcaac
     1561 aagccgttcc gggtgctgtg catcgccttt gtggtgatga acctgggcag ttcggtcgcc
     1621 acctcgacgc tggtcttctt catcgtccac tacctgcgac agccggaggt catcgggccg
     1681 gtcctgctcg gctcgttcgc ctcggtgctg gcgttcgtcc cggtatgggt gtggatcagc
     1741 cgcaggatcg gcaagcatcg cgcggccggc atctcgctga tcgccgcgat cctcatcaac
     1801 gcggtcgtgg ccctgctgct gcagccaggg gacggctggt ggttcgtcgg ggcgatgatc
     1861 gtcgcgggcg ccgccagcgc cggctacctg accctgccgc tcggcatgat gggcgacatc
     1921 atcgacttcg acgcgctcag gaccggccag aaccgcggcg gcctctactt cggcatctgg
     1981 tccttcgccc agaagatttc gcccgcgatc gccgtcggcg tcactcttcc gctcctgacc
     2041 tatgtcggct tcgaccccgc cagcaaggac ggcacggccg gcgtcacggc cctgaagtac
     2101 atcttcgccc tgggctcgac gccgctgttc ctgatcggcg ccctgctgct gatggccttt
     2161 ccgatcgatg cgcggcgaca cgccatcatc cggcggcggc tggacagcct ggccgaacgc
     2221 aacgcccgcg acgacggcgc ggaggagtga cgatcagcgg agcagatcgt cgacgaggtc
     2281 gctgaacccg ctctcaagcc tgcggatctt gcccgcgcga ccatcgccgg accagtcgga
     2341 cagaccggca aacgggccaa ccagaagggc ggtggcggca acgaagagcg cggcggacag
     2401 ggccgcggtc gtcgctgaca cgatcggcag acgcgctggc ggctcccgag cctcgccccc
     2461 gtcagccacc tgggtccctc cgcgtccagc cacgagactg gcagggcgcg cgccgtccgg
     2521 ccatcccaca ttcgtggaaa tccgggtccc gtcgcccacc tcgacggtag cgcggccgga
     2581 ggttctgtct cataccggtc gaacatcctc gctcaccgga caggctcatg accttcaagg
     2641 ccctcctcgc cagccgcatc gaccgcacgg tgtcggtcga ccttgtcgag atgacaaccg
     2701 accagctgat ggatggcgac gtcagcatcg acgtcgacta ctcgaccgtg aactacaagg
     2761 acggcctggc gctggccggg cggccgggcg tgatccagac ctatccgctc gtgcccggca
     2821 tcgacctggc cggcacggtc accgcctcga cccacgccgg ctacgtccct ggcgacaggg
     2881 tggtgctgaa cggctggggc ctcagtcaga cccatcatgg cggcttcgcc cagaaggcgc
     2941 gggtcagcgg cgactggctg gtcaagctgc cgcaagccat cagcacgcgc gacgccatgg
     3001 ccatcggcac ggccggctac acggcgatgc tggcggtgct ggcgctggaa cacgccggcg
     3061 tcacgccgga ccggggaccg gtgctggtca cgggcgccgg cggcggcgtc gggtcggtgg
     3121 cgatcctgat cctgtcccgg ctgggctacc gggtgatcgc ctcgacggga cggccggagg
     3181 agagccccta cctgaccgcc ctgggcgccg ccgagatcat cgaccgcaag gcgctgtcgg
     3241 agcccggacc gccgatcgct tcgccccgct ggatcggcgc cgtggactcg gtcggcagcc
     3301 agaccctggc caatgtgctg gcccagaccg actatcgcgg ggcggtggcg gcctgcggcc
     3361 tggcccaggg cgtcgacctg ccgggctcgg tattcccgtt catcctgcgc aacgtcaccc
     3421 tcgccggcat cgactcggtc aacgcgccgt tcgccgtcag ggcccgcgcg tgggaccggc
     3481 tggccaggga cctcgacctg tcccggctgg cgaccaccgt gaccgaggtc gggcttgccg
     3541 aggttccggc cgtgggccgc gccattctcg agggcagggt gcgcggccgc accgtggtgg
     3601 acgtgaacgc ctaagtcccg cagggccagg acccgcgccc cggacctgga aagggcctgg
     3661 ccctatcgcc cgctctggcc gtcgtcgaca cggatgcagg cgcccgtgac gaagtccgag
     3721 gccggcgaga cgaggaacag cagggtgctg tccagctgcg ccgggtcgcc cagtcgcttg
     3781 cgcgggaagt gctggctgat gtcgccgacc cgcgagagca tgccgtccat catctcggag
     3841 gcgaaggcgc ccggggcgat ggcgttgacg ttgatgttgg cgctcgccca ttcgaccgac
     3901 agggcctcgg tcatccgcac caccgccgac ttggttacgg cgtagagggc cgcgccgttg
     3961 ccgcggtact cgtaggccgc caccgaggcg atattgacga tccggcccgg cgacttcgcc
     4021 gccatcagcc ggcgggccac ctcgcaggac aggatgtagg gcgcccgcag gttgaccccg
     4081 atgacccggt cgatcaggtc gaccgacatc ttcgtcgccc tctgggcgtc ggggatgccg
     4141 gcgttgttga ccaggatggt caccgtgccg aacgccgcct cggccttgcc gaccaccgcc
     4201 agcaactggt cggcgtcggt gacgtcgagc tcgagggcga gggcctcgcc gccctcggcg
     4261 cggatctcgg cggccagggc ctcgagccgg tcgaggcggc gggcggtcag cgcgaccttc
     4321 gcgccgcatc tggccagcgt cctggcgaag cggaccccga ggcccgagga agcgccggtg
     4381 acgagcgcca cctggcccgt caggtcggtg gagacgttgg ggatcggata ggtcatggcg
     4441 gtctttcagg gtctgaaggt cagtcgcggc cttcgagaat ctcgatggcc tgtctggcga
     4501 ggtggggcgt gccgttctcg gcgggacggt cgctggcggc gttgccggcc aacacccggc
     4561 ggtagacgcc ctgcgagatc gaggcgaggc ggaagatcga gaaggccatg tagaacggcc
     4621 agtcctcgat cggccctcgg cccatccgcc ggcaataggc ggcgacgtac tccgcctcgg
     4681 tcgggatgcc ggacgcggcg aagtcgaccc cctgcagcgt gccccaggac ggcgaatgcg
     4741 agcgccagag gaaggcgttg taggcgatgt cggccagggg attgccgatg gtcgacagct
     4801 cccagtcgag caccgcgatc agcctcggct cggtcgggtg gaacatgacg ttctcgagcc
     4861 ggtagtcgcc gtgggcgatg gagaccggat cgtcctttgg gatgcgccgc ggcagctggg
     4921 cgatcagcgc ctccatttcc gggatcagct cgctctcggc gccccggtac tgcttcgtcc
     4981 agcggctgac ctggcgctcg aaatagccgc cggccttgcc gaagtcgccg aggcccgcgg
     5041 cggcgaagtc gaccttgtgc agcttcgcca gggtcgcgtt cagatcgtca tagatcgccg
     5101 cgcgctcggc gggctccaac cccggcaggg tcgcgtcgcg gaagatgcgg ccctcaaggt
     5161 agtccatgac gtagaaggcc gtgccgatga ccgtctcgtc ggtgcacagg gcgcgcatct
     5221 tcggcaccgg gaccagaccg tcgagggcct tcatcaccct gtattcacgg tcgacctggt
     5281 gggcgctggc cagcagcacg cccggcggct tcttgcgcag gacgtaacgg cgcgggccgg
     5341 cggcggtccg ggtggtcagc aggaaggtgg ggttggacgc gccgccctgg aactgctgca
     5401 cgatcatggc ctcgccgaaa tcctcgacct ggcggcgcag ccaggcttcc agcggggcgg
     5461 gatcaaagcg gtgcgcggcc gtcacgtcgc gcacgggcgg ggctgtcgcg gtcatgggag
     5521 actcctcttc cgccgatgac catgcccgct ttcccttcgg gcgccacaag agactctgtg
     5581 gcgggggcgg cgagaaaggg ggagcggagc ctggcgatca ggcggccgcg acgcggcgcc
     5641 ccggagtctg gaggcgccta tcgattgacg cccgcgcgtc ggcggaacgg gcccgtcccg
     5701 cagggttggg acgggccctg ccggcgttag aagcggaact ggccgaacag gccgaactcg
     5761 tcgtacgtcc cgtcgttgag gccgccgatg gtcccgttgg aggtgctgac gaagcccttg
     5821 tcgacggcgt cgcgcttgta gaccagcgac agatcgacga tcttggtggg gctgtaggtg
     5881 accccgacgt tgaaatagtc gtcctggcgg ttcggcgcgg tgtcctggtt gggctccacg
     5941 cggtcgtagc gggcgaaggc cccgatggtc ggcgtgaact ggtaggagcc gaacaccgag
     6001 tagccctcgg cgctgtcgga ggtcacggtc gagacgttgt tccagtcctc ggccttgaag
     6061 tactcgacgc cgacgcggaa ctggctggtg gtgtaggcgg ccatggcgtc gaagcggttg
     6121 gccgtgtgga aggtggtcac gccctgcttc gacttgccca gcttgccggt gtagccgccg
     6181 acggccagcg tcaggtgctc gaccggggtg atgctgatgc ggccttcgac atccatgccc
     6241 ttggagcgca gcggcgcctt gtagccggcg ccgtcgacca cggcgaccgc gtagctgacc
     6301 atgccgcccg ccagcttgcc cgaggcgtgc acgccccagt cggccgaggt gccgaacttg
     6361 gtccggtcga tcagcgtgtt ttcaacatag cggtagccgt acatgtcctc ggcgaacggc
     6421 acccagggca ggtccgtcga accgaggcgc acgaccaggg cgtcggagaa tttggcctgc
     6481 agataggcct tcttgatgta gagctcggtc gaggagatcg ccgacgaata ctggaagtcg
     6541 gtggtcacat tgcccgagaa catgtcgttg aacttgtgat caacgccaat gtagaagcgc
     6601 ttgatgtcga agcccgtgcc ggtcggctgg acgcgcgagc cgtccgactc ctgggtgatg
     6661 ttggtcaggt cgaagtacat ccggcccgac acgacggtct tgtcggtcca ggacggcttg
     6721 gccggcgcgg gcgccggcgg cggaacggcg gcgacggccg tctgaacctg ggccggaatg
     6781 gtctggatct gcgcgtcggc gcgggcctgg gcggcctcct gggcggcgag ccggtccgac
     6841 agggcctgga tctgcgcccg cagctgggcg atctcggcgg cgtcggcgcc ggtttcagcg
     6901 gcctgtgccg gcatcgcgat tgcggcggcg gcgatcatcg gactcaagag cacaccgaag
     6961 aacggataca gcacgccggc cgctatcggc acgccgagtg cgttnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatgcagtcg ggcgtgcggc gatagatcag
     7201 gccgatgcga cgtggcgaca ggcccgagcg cgcgagccga cggatgtcgg cgatctccgc
     7261 ctcaccgaga aggccgcgcg cgccttcggg cgggcgggcg ggcgacgcgg cgccgtcgcc
     7321 gtccagcctg gcctgaagat ccgagagcag gtgcagaacc gggccggcct cggcctggcc
     7381 atcgacccgc accacctggt cgtcgtcatc gagggtcagg caggcgacgc cgcggtcggt
     7441 gagggccttg agcagccgga ccgtctcgcc gacgcccagg cgaagcgtcg ccagcgagag
     7501 gaaacagacc tcgtcacctt cggcctgacg ctcgaggcat tcgagaatcc gctgcgcgga
     7561 ccgcgccgtc atcgtctcct cgacgtgata gcgctccggc gacagcgcct ccaggcgcgc
     7621 cagctggcgc tcaaggggcg gatggccggg cccgatctga atgaccgctg tgcgcatgcc
     7681 aaaagggtgg ccagccacgt cgggcaagtc aagctggact tacagtgcgg ctcccgacga
     7741 aagagcggcg cccgccattg aagaaaaact gtcatcagcc tgaagcatga tggtcaccgg
     7801 cccgccgccc ccggcgaacc ctggagcccg cgtcaatgcc ccctctgtcg acatcgactc
     7861 cggccgcgcg ctacggcgcg acgctcgccc tcgtggggct gggcttcgcc ggccggctgg
     7921 cgctggcccc gctgttcggc gacagccaca ccttcaccgt cttctatccg gtggtgatcc
     7981 tggccgccta cttcttcgga gccggaccgg cgaccctgtc ggcggttgtg tccgcgggac
     8041 tggcttactg gttcttcgcg gctccggcgc aggcctggaa gggggacgtc gccaacctca
     8101 ccaccacgct gctgttcgcc ttcaccgcca cggtgtcgat ctacttcatc accggcatgg
     8161 cgcgcgccct cgaccagctg gccgccgccc gggcgcgggc cgaggccctg gcccgcagcc
     8221 atgctgaact gttcggcgag ctcaacgagc gggtgaccaa ccatctgcag cttgtcgccg
     8281 ccctgctgca gttgcaggcg cgcgacgaga ccgaccagtc gctgtccaag gcgctcgccg
     8341 aggcctcggc ccggaccatc ctgatctccc gcgtgcatcg caccgtcgcc ggggaggcgg
     8401 acaagaccct cgacttcgac gtcttcgccc gccagctgct cgacgcgacc ctggccgccc
     8461 gcgggcatcc gccggtgcgg gtggaaatcg aggaacaggg tgtgcgcctg ccgctggacc
     8521 aggcgacctc ggtggcgatc gtcctgctcg agtgcttcaa cgcgcgcctg cagctgaaca
     8581 cccccgccct gatgcgggtg cggctgcggg agaccgatcg ggaggcgacg ctggaggtcg
     8641 ccgaggcccg cccccgcggc gaggatctgc ctgaactgga cctcaggcgg cagtcgatcg
     8701 aggcgatggt cgaacagctg ggtggccgtt tctcccaccg cagtgacgac gacggagcct
     8761 tgtcggccct ggtgtttcct cgtcccgggg ccggggcggc gccaatggcg gtcggccccg
     8821 ctgccgcgcc cacgctgcat tgaggacccg atggcgatcc cttcggcgcg gcccgcaaat
     8881 cacgctaggc tggcgcggtc cgccgcccgg cgcttcagac tgaccgcatg acagatcgtc
     8941 cgccgccctt ttcagccctg cgcgccgtcg aggccgcatc gcgccaccgc agcttcacct
     9001 gggcggcgaa ggagctgcgc atcacccaca gcgccgtcag ccagtcgatc cggcggctgg
     9061 aggccgagct tggcaccccg ctgttccagc gcaaaggcgg ggccatggag ccgtccgagg
     9121 ccgccctgcg cctggctgaa tcctatcacg acgccgcgcg ctcgctcgac cggaccctgc
     9181 gggagatcac cggcggctcc cgcgccggcg tgctgaccgt cgccatgccg gcggacttcg
     9241 gccgcctgtg gttcggcgcg aaactggcgc ggctggccga ggccatgccc gacctgagga
     9301 tcgaggtccg aacgaccggc tcgcgtgacc aggatccgga cagcgacgtg gtcatcgacc
     9361 tggcgacgac cgaagggcgc gaagccgatg tcgtcgggcc ggaactatcc tgttttcccg
     9421 tatgcagtcc cgaccttctg tcggcccggc gcctcgacac gccccggcag atcgtccgcg
     9481 cgccgctgat cgccggcggc ggttgcgact gggacggatg gaccgaacac ttcgccgtcg
     9541 cgagtcggcc accggccttc gccttcgacg acgccggcat ggcgctcgac gcgaccgctc
     9601 gcggagccgg cgtggccctg actcacctgt tcgccgccga ggggcacctc gacgacggcc
     9661 gactcgtcgc cctgcccttc gcggttccga ccggccgtca tctgctgctg agcgccgggc
     9721 ccggccaggc ggatctggcc gcccggttcg ccatgt
//
LOCUS       DAORNX010000054         9650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5214_length_9650_cov_7.351433, whole genome shotgun sequence.
ACCESSION   DAORNX010000054 DAORNX010000000
VERSION     DAORNX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_9650_cov_7.351433"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLO65_03830"
     CDS             complement(<1..1112)
                     /locus_tag="PLO65_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRD27412.1"
                     /translation="MKKSNLKGAISIGALLMGLASGGAAVAQESGETTVGEVVVTANR
                     REQSVLEVPYNISAVSGEAIEDGKMLDAAELMRGVPGVAVVDRGYRNGGVINNVLIRG
                     INVDSNALGDYAVSSVAPVSTYQNDTPLFAGFLLKDLERVEVLRGPQGTLYGSGSLGG
                     TVRYITRAPRLGEFSGAANVTASKVEGSGSVGWAGDLTLNLPLGDMFALRATVSRIDY
                     PGLTDYVNLYVLDATGAPAAPGGLLSPDAAYTSRKDADTVEITFARAALLFEPNDTFN
                     ATLTYSQQSDEVGGRRQVTRGLDGYGNRYGDYENGSVQLEPSSRDVSLAALEMNIDLG
                     FATLTSSTSVYSHEGDSVSENTGFYAQAGFLSFYYN"
     gene            complement(1187..2074)
                     /locus_tag="PLO65_03835"
     CDS             complement(1187..2074)
                     /locus_tag="PLO65_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRD27413.1"
                     /translation="MRPIGIAGLQLQLDRGDNGDRIAAEVAAVKARLPWVDMVVLGEL
                     ALFGAAVANAQPLPGEAEARMCEAARAAGVWLVPGSLFEADGGRVYNTAPVIDPSGQV
                     VARYRKIYPFLPYEQGVTPGNEFVVFDVPAVGRFGLSICYDMWFPETTRTLTWMGAEV
                     ILHPGMTNTIDREAELAIARASAVTNQVFFVDINTAGSLGVGLSGVFGPGGEVIHQAG
                     AGHEVIAVELDLDQVTRVRERGWHGLGQPLKSLRDHPIDFPPYREGFAASPAFSGLGP
                     LQKPGLARPASPGLRVIKT"
     gene            complement(2216..4036)
                     /locus_tag="PLO65_03840"
     CDS             complement(2216..4036)
                     /locus_tag="PLO65_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRD27414.1"
                     /translation="MSRPLLFLDPDSDLSLQDQVRRKLVDAACAGAFPPGKRLPSSRA
                     LAAQLGVARNTVTLAYQKLLDEGLFVSRERSGIYADHDMLRGLTAVLSVAGDREPAPS
                     TRWRGRFKSGEGDEPVARRTPNWRQYPYPFIDGLFDASLFPVAEWREASRQALAVGEI
                     GQWSMDGGDADDPMLIEEIRTKVLPRRGIQARPDEILVTVGAQQALSLVSRMLIDRTS
                     VVAIENPGYPDMRRLAMDRGARLVHQPVDDEGLVVDRRLDDCDLIYVTPSHQFPTGVM
                     MTQARREALLRKAAARDLIIIEDDFACETNYLDEACPALRSLDRDDRVIYVAGFSKVL
                     GPGLRLGFMVASAEVIAEARRLRHLSIRHPPLNNQRTAAHFLAMGHYDATMMRLGRLF
                     RERRTALRDALNHYLQQSVAIAPLRGGATYWVRGPDHLDVEAFAAEAERRGVLIEPVG
                     PYYADNKAPTNIFRLGVTSLPLDRIRHGVEVLADLMRDLPGTARTFPETAEARLSGEA
                     LRAAMSGAVLLCKTVYGDPCTIELLPDGRMVGRAGYANEDCDEGRWWVEGDFWRRQWD
                     RWSYGETSSLMTTIVGDRIGWFDAGGRLVDSAVIRRAEAA"
     gene            4455..6161
                     /gene="kdpA"
                     /locus_tag="PLO65_03845"
     CDS             4455..6161
                     /gene="kdpA"
                     /locus_tag="PLO65_03845"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRD27415.1"
                     /translation="MDWRGWAEIVFTIGLTLALSLPLGGYLARVWTGERTWLQPVLRP
                     VERVAYALFGVDSEKGQGWLAYTGSVLAFSGAGFAILYGMLRLQGLLPLNPQGFAGLS
                     PHLAFNTAVSFVTNTNWQSYAPEATLSTFSQMAGLTVQNFVSAATGLAIAAAVARAFA
                     ADRGDRLGNFWVDVTRIVLYVLLPVSLVGALVLVALGVPQSLAAGAEATTLEGAKQTL
                     ALFPVASQEIIKQFGTNGGGVFNANSAHPFENPNAWTNLIQIVAMNVLSLATVFAFGR
                     VVLARKEARALAAVMVILVAAAAGGVYATETLTPQPAMIAAGVDAPVSMEGKEVRFGA
                     PSTAVWAAFTTGASDGGVNGMHASFTPLGGGIALFMIQLGEILPGGVGSGLYGMMVMA
                     LLAVFVAGLMVGRTPEYLGKKVEAREIKFAMLAVLILPIVILGFSAMAAVLPVALAGL
                     LNKGPHGLSEIMYAYSSAAGNNGSAFAGLTANAPWWNTTLGIAMLLGRFAYIVPVMAI
                     AGSIAAKPKLAPTAGTLPSDGPLFAGLLIGIILILGGLQFFPALALGPIVEHFQVLAA
                     AR"
     gene            6184..8253
                     /gene="kdpB"
                     /locus_tag="PLO65_03850"
     CDS             6184..8253
                     /gene="kdpB"
                     /locus_tag="PLO65_03850"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRD27416.1"
                     /translation="MSTMDPTADGPRTKSAIGSVTLSRAALFRAMREAFLKLDPRKLT
                     GNPVILSTEVVAVLATVSAGVAIGAGQPAGFPVQIAAWLWATVLFANLAESVAEGRGK
                     AAADSLRAARVTTKAKLLIDPSTGTWIPTPAHKIGAGEVVLVEAGDVIPADGEIIEGV
                     ASVNEAAITGESAPVIRESGGDRSAVTGGTTVVSDWIKVRITSQPGSTFLDRMIAMVE
                     GADRRKTPNELALAVLLAGLTLVFLIAVVTLLGLGAYSGVRLDPMVLGALFVTLIPTT
                     IGGLLSAVGIAGMDRLLKVNVLATSGRAVEAAGDVDTLLLDKTGTITFGNRMATEVIP
                     APGVRPEVAMKAALMASLADETPEGRSIVDLARDQGLTAEVPAGATFIAFSATTRQSG
                     LDAGKESWRKGAVDAVLRSLNLSEAQTPPEFRQAVDRIARSGGTPLAVTENGVLVGAI
                     HLKDVVKPGVKDRFADLRRMGVRTMMITGDNPVTAAAIASEAGVDDFLAEATPEDKLR
                     LIRAEQAKGRLVAMCGDGANDAPALAQADVGVAMQTGAQAAREAGNMVDLDSDPTKVL
                     EIIEVGKQLLITRGALTTFSIANDVAKYFAIIPALFVVALPALGGMNIMQLHSPESAI
                     LSAVIFNALVIVALIPLALRGVKYRAIGAGKLLSRNLTVYGLGGLVAPFIGIKAIDLI
                     VSAIGLA"
     gene            8265..8848
                     /gene="kdpC"
                     /locus_tag="PLO65_03855"
                     /pseudo
     CDS             8265..8848
                     /gene="kdpC"
                     /locus_tag="PLO65_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
     gene            8845..>9650
                     /locus_tag="PLO65_03860"
     CDS             8845..>9650
                     /locus_tag="PLO65_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRD27417.1"
                     /translation="MSPDEHPRPDPDALLATAGRHGRGRLKVFLGMSPGVGKTYAMLG
                     AAARHRAGGGDVLVGVIETHGRRETEALLDDLEVLPRKPIDYRDRTLLEFDLDAALAR
                     RPGLLLVDEYAHSNAPGSRHPKRWQDVEELLAAGINVWTTLNVQHLESLVDVVWKITG
                     VRQRETVPDSALSRADEIELIDITPTELRERMAAGKVYVPETARLAADRFFKPENLTA
                     LRELALRRAAQTVDDQLNQAMRAAGVEGPWAAGERILVLVGPDAMAGSLVR"
BASE COUNT         1399 a         3476 c         3311 g         1464 t
ORIGIN      
        1 tagttgtagt agaagctcag gaagccggcc tgggcgtaga agccggtgtt ctcgctgacg
       61 ctgtcgccct cgtggctgta gaccgaggtc gacgaggtca gggtcgcgaa gcccaggtcg
      121 atgttcattt ccagggcggc caggctgacg tcgcgcgacg acggctcgag ctgcaccgac
      181 ccgttctcgt agtcgccgta gcggttgccg tacccgtcca ggccgcgcgt cacctgccgg
      241 cgaccgccga cctcgtcgga ctgctggctg taggtgaggg tggcgttgaa cgtgtcgttc
      301 ggttcgaaca gcagggcggc gcgggcgaag gtgatctcga ccgtgtcggc gtccttcctg
      361 ctggtgtagg cggcgtcggg cgacaggagg ccgcccggcg cggccggggc gccggtggcg
      421 tccagcacat agaggttcac gtagtcggtg aggcctggat agtcgatccg gctgaccgtg
      481 gcgcgcaggg cgaacatgtc gccgagcgga aggttgaggg tcaggtcgcc ggcccagccg
      541 acgctgccgg agccctcgac cttgctggcc gtgacgttgg ccgcgccgct gaactcgccc
      601 agtcggggcg cgcgggtgat gtagcggacg gtgccgccga gcgagcccga gccatagagg
      661 gtgccctgcg gcccgcgcag cacctcgacc cgctcaagat ccttgagcag aaagccggcg
      721 aacaggggcg tgtcgttctg gtaggtcgac accggggcga cgctggagac cgcgtagtcg
      781 cccagggcgt tgctgtcgac gttgatcccg cggatcagca cgttgttgat cacgccgccg
      841 ttgcgatagc cgcgatccac caccgcgacg cccggcaccc cccgcatcag ctcggcggcg
      901 tcgagcatct tgccgtcctc gatcgcctcc ccggacaccg ccgagatatt gtagggcact
      961 tccaggacgc tctgttcgcg gcggttggcc gtgaccacga cctcgccaac ggtggtctcc
     1021 ccggactcct gggcgacggc ggcgccgccg gaggccaggc ccatgagcaa cgcgccgatc
     1081 gaaatggcgc ccttcaggtt cgacttcttc atctcaatcc ccctcctatc agacagacct
     1141 gctgtcattt cccgccagac gcgcgacgcg cgcgcccgcg acgcgcctac gtcttgatca
     1201 ctcgcaaccc gggcgaggcc ggccgcgcca accccggctt ctgcaatggt cccagtccgg
     1261 aaaaggccgg cgacgcggcg aacccttccc ggtagggcgg aaagtcgatc ggatggtcgc
     1321 gcaggctctt caggggctgt cccagcccgt gccagccgcg ctcgcgcacc cgcgtcacct
     1381 ggtccaggtc cagctcgacg gcgatgacct catggccggc gcccgcctga tgaatgactt
     1441 cgccgcccgg cccgaacacg cccgacaacc cgaccccgag ggagccggcg gtgttgatat
     1501 cgacgaagaa gacctggttg gtcaccgcgc tggcgcgggc gatggccagc tccgcctcgc
     1561 ggtcgatggt gttggtcatg cccgggtgaa gaatgacctc ggcgcccatc caggtcaggg
     1621 tgcgggtcgt ctccgggaac cacatgtcgt agcagatcga caggccgaag cggccgacgg
     1681 cgggcacgtc gaagacgacg aactcgtttc cgggggtgac gccctgctcg tagggcagga
     1741 acgggtagat cttgcggtaa cgggcgacga cctggccgct cgggtcgatc accggcgcgg
     1801 tattgtagac gcggccgcca tcagcctcga acagcgaacc gggcaccagc cagacgccgg
     1861 cggcgcgggc cgcctcgcac atcctggcct cggcctcgcc gggcaggggc tgggcgttcg
     1921 ccacggcggc gccgaacagg gccagttcgc cgagcaccac catgtcgacc cacggcaggc
     1981 gcgccttcac ggccgcgacc tcggcggcga tgcggtcgcc gttgtcgccc ctgtcgagct
     2041 gcagttgaag gccggcgatt ccaatcggac gcatgagaac tctctgggac aggccgaggg
     2101 ccatgaacca gcagagtggc cgcaggggcc gccgtcacat agcgccagcc cgcgcccgtt
     2161 tatggcgcca gtttgacacc gatatgagcg accgatgaaa tctggaccca gagggtcagg
     2221 ccgcctccgc ccgccggatg acggcggagt caaccagccg cccgccggcg tcgaaccagc
     2281 cgatgcgatc gccgacgatg gtggtcatca ggctcgaggt ctcgccgtag gaccagcggt
     2341 cccactggcg acgccagaag tcgccctcga cccaccagcg cccctcgtcg cagtcctcgt
     2401 tggcgtagcc ggcccggccg accatccgcc cgtcgggcag aagctcgatc gtgcaggggt
     2461 cgccatagac cgtcttgcac agcagcaccg cgcccgacat ggccgcccgc agcgcctcgc
     2521 ccgacagcct ggcctcggcg gtctccggga aggtcctggc cgttcccggc agatcccgca
     2581 tcaggtcggc gagaacctcg acgccatggc ggatccggtc cagcggcagg ctggtcaccc
     2641 cgagccggaa gatgttcgtc ggggccttgt tgtcggcgta gtagggcccg accggctcga
     2701 tcagcacgcc ccggcgctcg gcctccgcgg cgaaggcctc gacgtcgagg tggtcgggac
     2761 cccgcaccca gtaggtcgcc ccgccccgca gcggcgcgat ggcgaccgac tgctgcagat
     2821 agtggttgag ggcgtcgcgc agcgccgtgc ggcgctcgcg gaacagccgg cccagccgca
     2881 tcatcgtcgc gtcatagtgg cccatggcga ggaaatgggc ggccgtccgc tggttgttca
     2941 gcggcgggtg gcggatggac aggtggcgca gccggcgggc ctcggcgatc acctcggccg
     3001 aagcgaccat gaaccccagc cgcagccccg gtcccagaac cttggagaag ccggcgacat
     3061 agatcacccg gtcgtcgcga tccagactgc gcagggccgg gcaggcctcg tcgaggtagt
     3121 tggtctcgca ggcgaaatcg tcctcgatga tgatcaggtc gcgcgcggcg gccttccgga
     3181 gcagcgcttc ccggcgggcc tgggtcatca tcacgccggt gggaaactga tggctcggcg
     3241 tgacgtagat gaggtcgcag tcgtccagcc gccggtcgac caccagcccc tcgtcatcca
     3301 ccggctggtg aaccagccgg gcgccgcggt ccatggccag ccggcgcatg tccgggtagc
     3361 cgggattctc gatcgccacc acgctggtcc ggtcgatcag catgcggctg acgagcgaca
     3421 gcgcctgctg ggccccgacg gtgaccagga tctcgtccgg acgcgcctgg atgccccgcc
     3481 gcggcagcac cttggtgcgg atctcctcga tcagcatcgg atcgtcggcg tcgccgccgt
     3541 ccatcgacca ctggccgatt tcgccgaccg ccagggcctg ccggctggct tcgcgccatt
     3601 ccgccaccgg gaacagggac gcgtcgaaca gaccgtcgat gaagggatag ggatactgcc
     3661 gccagttggg cgtccgccgc gcgaccggct cgtccccttc gcccgacttg aagcggccgc
     3721 gccagcgcgt cgagggcgcc ggctcgcggt cgccggccac ggacagcacc gccgtcagac
     3781 cgcgcagcat gtcatggtcg gcgtagatgc cgctgcgctc gcggctgacg aacaggcctt
     3841 cgtcgagcag cttctgatag gccagggtca ccgtgttgcg cgccacgccg agctgggccg
     3901 ccagcgccct ggacgacggc agccgttttc caggcgggaa ggccccggcg caggccgcgt
     3961 cgaccagctt gcggcggacc tggtcctgca ggctgagatc gctgtcggga tcaaggaaca
     4021 gaagcgggcg ggacatgccc ggggttttcg cccgtcgccg tcggccgagt ccagtgcgac
     4081 ccgccgccgc gcgcgtgtga ccggcgggcc tggcgacacg gggcggccgt tgacgtgaat
     4141 ccagtcaccc ccgcgccggg cccttatggt ccccttacgc caggggacca tcatccgtct
     4201 ggctccctga cggccgtcgg cgcgatcttt cgcgtgacat cgttgtggag gccctgatgg
     4261 ccgatctcct gtttctcctg ctcgggctcg gggcgttcgc gctcttcggg ctctatgccg
     4321 cgctgctgcg caaggtctga cgaccatgct cacaacgatc ctctacgccg ccgccgccgt
     4381 cggactggcc gtctacatgg tcgccgcgct gctgcgcccc gacaaattct gatcaaaccg
     4441 aagggagacc tcgcatggac tggcggggct gggccgagat cgtcttcacg atcggcctca
     4501 cactggcgtt gagcctgccg ctcggcggct atctcgcgcg ggtctggacc ggtgaacgca
     4561 cctggctgca gccggttctt cggcccgtcg agcgggtcgc ctatgcgctg ttcggcgtcg
     4621 acagcgaaaa gggccagggc tggctggcct acaccggttc ggtgctcgcc ttcagcggcg
     4681 cgggctttgc gatcctgtac ggcatgctgc ggctgcaggg cctgctgccg ttaaatccgc
     4741 aggggttcgc cggcctgtcg ccgcacctgg ccttcaacac cgccgtcagc ttcgtgacca
     4801 acaccaactg gcagtcctac gcgccggagg cgacgctttc gaccttctcg cagatggccg
     4861 gtctgacggt gcagaacttc gtctccgccg ccaccggact ggccatcgcc gccgccgtcg
     4921 cccgcgcctt cgcggccgac aggggcgaca ggctgggcaa cttctgggtc gatgtgaccc
     4981 gcatcgtcct ctacgtcctg ctaccggtca gcctggtcgg cgccctggtg ctggtggcgc
     5041 tcggcgtgcc ccagtcgctg gcggccggcg ccgaggcgac gacccttgag ggggcgaagc
     5101 agacgctcgc cctgttcccg gtcgccagcc aggagatcat caagcagttc ggcaccaacg
     5161 gcggcggcgt cttcaacgcc aattccgcgc acccgttcga aaatcccaac gcctggacca
     5221 acctgatcca gatcgtggcc atgaacgtgc tcagcctggc caccgtgttc gccttcggcc
     5281 gggtggttct ggcccgcaag gaggcccgcg ccctggcggc cgtcatggtc atcctggtgg
     5341 cggccgcggc cggcggcgtc tacgcaaccg aaaccctgac gccgcagccg gcgatgatcg
     5401 ccgccggcgt cgacgcgccg gtcagcatgg aaggcaagga ggtccgcttc ggcgcgccct
     5461 cgacggccgt ctgggccgcc ttcaccaccg gcgcctccga cggcggcgtc aacggcatgc
     5521 acgccagctt cacgccgctc ggcggcggca tcgccctgtt catgatccag ctgggcgaga
     5581 ttctgccggg cggggtcggc tcgggcctct atggcatgat ggtcatggcc ctgctggcgg
     5641 tgttcgtcgc cgggctgatg gtcggccgca cgcccgagta tctcggcaag aaggtcgagg
     5701 cgcgcgagat caagttcgcc atgctggcgg tgctgatcct gccgatcgtg atcctgggct
     5761 tctcggccat ggcggcggtg ctgccggtgg ccctggccgg gctgctcaac aaggggccgc
     5821 acggcctgtc cgagatcatg tatgcctaca gctcggcggc ggggaacaac ggctcggcct
     5881 tcgccggcct gaccgccaac gccccctggt ggaacaccac cctgggcatc gccatgctgc
     5941 tgggccgctt cgcctacatc gtgccggtga tggccatcgc cgggtcgatc gcggccaagc
     6001 ccaagctcgc gccgaccgcc ggcaccctgc cgtcggacgg cccgctgttc gccggcctgc
     6061 tgatcgggat catcctgatc ctcggcggcc tgcagttctt ccccgccctg gcgctgggtc
     6121 cgatcgtcga gcacttccag gtgctggcgg cggcgcgctg agctcccttc aaggacgcct
     6181 gacatgtcca ccatggatcc aaccgccgac gggccgcgca ccaagagcgc catcggctcg
     6241 gtcaccctct cccgcgccgc cttgttccgg gcgatgcgcg aggccttcct caagctcgat
     6301 ccgcgcaagc tgaccggcaa tccggtaatc ctgtcgaccg aggtggtggc cgtcctggcc
     6361 actgtctcgg ccggcgtcgc catcggcgcg ggccagcccg ccggcttccc ggtccagatc
     6421 gccgcctggc tatgggccac cgtgctgttc gccaacctgg ccgaaagcgt cgccgagggc
     6481 cggggcaagg cggcggccga cagccttcgc gccgcgcggg tgaccaccaa ggccaagctg
     6541 ctgatcgatc cctcgaccgg cacctggatc ccgacgccgg cgcacaagat cggcgccggc
     6601 gaggtggttc tggtcgaggc cggggacgtg atcccggccg acggcgagat catcgagggc
     6661 gtggccagcg tcaacgaggc ggccatcacc ggcgagtccg cgcccgtgat ccgcgagagc
     6721 ggcggcgacc gctcggccgt caccggcggc accaccgtgg tctccgactg gatcaaggtg
     6781 cgcatcacca gccagcccgg ctcgaccttc ctcgaccgga tgatcgccat ggtcgagggc
     6841 gccgaccggc gcaagacgcc caacgagctg gccctggccg tgctgctggc cggactgacc
     6901 ctggtgttcc tgatcgcggt cgtgaccctg ctgggcctgg gcgcctattc aggcgtcagg
     6961 ctcgacccga tggtgctcgg cgccctgttc gtcacgctca ttccgaccac catcggcggc
     7021 ctgctcagcg ccgtcggcat cgccggcatg gaccggctgc tgaaggtcaa tgtgctggcc
     7081 acctccggcc gggccgtgga ggcggccggc gacgtcgaca ccctgctgct cgacaagacc
     7141 ggcacgatca ccttcggcaa ccgcatggcc accgaggtca ttccggcgcc gggcgtgcgg
     7201 cccgaagtgg cgatgaaggc cgccctgatg gcctccctgg ccgatgagac cccggaaggc
     7261 cgctcgatcg tcgacctcgc ccgcgaccag ggcctgaccg ccgaggttcc cgccggcgcg
     7321 actttcatcg ccttctcggc gacgacccgg caatcgggcc tcgacgcggg caaggagtcc
     7381 tggcgcaagg gcgcggtcga cgctgtgctt cggtcgctga acctgagcga ggcccagacc
     7441 ccgcccgagt tccgccaggc cgtcgaccgc atcgcccgct ccggcggcac gccgctggcg
     7501 gtgaccgaga acggcgtgct ggttggcgcc atccatctga aggacgtggt caagcccggc
     7561 gtgaaggacc gtttcgccga cctgcgccgg atgggcgtgc ggacgatgat gatcaccggc
     7621 gacaaccccg tgaccgccgc ggccatcgcc tccgaggcgg gggtcgacga cttcctcgcc
     7681 gaggccacgc ccgaggacaa gctgcgcctg atccgcgccg aacaggccaa gggccggctg
     7741 gtggccatgt gcggcgacgg ggccaacgac gcccccgcgc tcgcccaggc cgacgtcggg
     7801 gtggccatgc agaccggggc ccaggccgcc cgcgaggccg gcaacatggt cgacctcgac
     7861 agcgacccga ccaaggtcct ggagatcatc gaggtcggca agcagctgct gatcacccgc
     7921 ggcgccctga cgaccttctc gatcgccaac gacgtggcca agtatttcgc catcatcccg
     7981 gcgctgttcg tggtcgccct gccggccctg ggcggcatga acatcatgca gctgcacagc
     8041 ccggaaagcg cgatcctgtc ggccgtgatc ttcaacgccc tggtgatcgt ggccctgatc
     8101 ccgctggctc tgcgcggcgt gaagtaccgg gccatcggcg ccggcaagct gctcagccgc
     8161 aacctgaccg tctacggcct gggcggtctc gtcgccccgt tcatcggcat caaggcgatc
     8221 gacctgatcg tctccgccat cggcctcgcc tgaggactgt gaccatgctc tcgcacctgc
     8281 gtcccgccat cgtctcgatg gtcttcttca ccgccctcct cggcctgggc tatccgctgg
     8341 ccgtcaccgg catcgcccag gcggccttcc cggcccaggc caacggctcg ctggtcaccg
     8401 acaaggccgg caagacggtc gggtcggccc tgatcgccca gggcttcacc caggaccgct
     8461 acctgcatcc gcgtccctcg gcggccggca agggctatga cggagccggg tcctcgggct
     8521 cgaactacgg cccgctgaac gcggactacg ccgcccgggt gaaggccgac gccgacgccc
     8581 tgcgggcgga gagcccggcc gccgttccgg tcgacgcggt gaccacctcc ggctccggcc
     8641 tcgacccgga tgtctcgccg gccaacgccg aacgccaggt gcagcgcatc gccgccgcgc
     8701 gcggcgcgag gcccaggtcc gcgccgtgat cctcgccagc accgtcgccc cgacgctcgg
     8761 ctttcttggt cagccgcggg tcaatgtgct gaaggtcaat ctggcgctcg acgcccggct
     8821 tccggcggcg caacctccgg cgcgatgagc ccggacgagc accccagacc cgatccggac
     8881 gcgctgctgg cgacggcggg gcgtcacggc cggggacggc tgaaggtctt cctcggcatg
     8941 tccccgggcg tgggcaagac ctacgccatg ctcggcgccg ccgcccgcca cagggccggc
     9001 ggcggcgacg tcctggtcgg ggtgatcgag acccatggcc ggcgcgagac cgaggccctg
     9061 ctggacgacc tggaggttct cccacgcaag ccgatcgact accgcgatcg cacgctgctc
     9121 gagttcgatc tcgacgccgc gctggcgcgc cggccgggcc tgctgctggt cgacgagtac
     9181 gcccattcca acgccccggg ctcgcgccat cccaagcgct ggcaggacgt cgaggaactg
     9241 ctggcggccg ggatcaatgt ctggaccacg ctgaacgtcc agcatctcga gagcctggtg
     9301 gacgtggtct ggaagattac cggcgttcgc cagcgcgaga cggtgcccga cagcgcgctg
     9361 tccagggccg acgagatcga gctgatcgac atcaccccga ccgagctgcg cgagcggatg
     9421 gcggcgggga aggtctatgt ccccgagacg gcccgactgg ccgcggaccg gttcttcaaa
     9481 cccgagaacc tgaccgccct gcgcgagctg gccctgcgtc gcgcggccca gacggtcgac
     9541 gaccagctga accaggccat gcgggcggcc ggggtcgagg ggccgtgggc ggccggcgaa
     9601 cgcatcctgg tgctggtcgg gccggacgcc atggcggggt cgctggtgcg
//
LOCUS       DAORNX010000055         9643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5219_length_9643_cov_6.829057, whole genome shotgun sequence.
ACCESSION   DAORNX010000055 DAORNX010000000
VERSION     DAORNX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_9643_cov_6.829057"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLO65_03865"
     CDS             <1..214
                     /locus_tag="PLO65_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27418.1"
                     /translation="ARARASVSAAFDAARDAVKAMTAVAGILDAGLFKRQYRDHRLEW
                     MIRSGGVEVVLRGLEKGDIRFNDEPV"
     gene            347..1849
                     /locus_tag="PLO65_03870"
     CDS             347..1849
                     /locus_tag="PLO65_03870"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD27419.1"
                     /translation="MTDTATLSRAATTTTEHFDVLIVGAGISGVGGAYHLKTQCPGTS
                     YVVLEGLENFGGTWWMHRYPGIRSDSDLYTFGYRFKPWVGAPIASAEEILKYMGEVID
                     ENDLARHIRYGHQITSATWSTSDRKWTIEGVRKDTGQPVRFTCNFLWMCQGYYRHSEG
                     YTPEWEGMDTFKGKIVHPQTWPQDLDYRNKKVIVIGSGATAATLVPAIAGDTAHTTLL
                     QRSPTYFIPGRNENELANTLRELEIPEEWIHEIVRRKVLFDQAAFTKRAVEESEAVKN
                     ELLEGVRAFVGPDFDIEKHFTPKYRPWRQRIAFVPDGDLFQGVVSGKASVVTDEIDRF
                     TEKGILLKSGQELEADIIVTATGFNLSVLGDIRFVIDGKPLNFHDTVTYRGMMFTGVP
                     NMVWVFGYFRASWTLRADLIGDFVARLLNHMKKKGATMVTPTLRPEDRDMPLSDWMDP
                     ENFNPGYLMRGMHLLPKKGDKPEWQHTQDYWAEKDEIPAIDLDDAAFVYR"
     gene            complement(1905..2282)
                     /locus_tag="PLO65_03875"
     CDS             complement(1905..2282)
                     /locus_tag="PLO65_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27420.1"
                     /translation="MFSHIMIGTNDLERAKSFYDALLGTLGVPPAFVDGHRIFYMTPT
                     GVFSVSKPINGEPATAPNGGTIGFACSSPEVADAWHAAGLAAGGTTCEDPPGVRAGAT
                     GKMYLAYLRDPDGNKLCGLHRMQ"
     gene            complement(2502..3176)
                     /locus_tag="PLO65_03880"
     CDS             complement(2502..3176)
                     /locus_tag="PLO65_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27421.1"
                     /translation="MERHSPRIDPRRARTRRALLRAGEALLISHAIEVVSVDDIVKTA
                     GVTRGSFYNHFEDKEALAREISRNVRLAGEAMVDAANEGISDPAERVARALAIFVHFS
                     TVEPLRARSMVRLFAGATALPLADVNIGVLADVARGLEEGRFRGPSLQSGILLIIGIV
                     QMSILYLLESTRPVATEAFAAELAADLLRGLGVDPTQVTAIGRRAARDVFADGARLQA
                     RLDPLD"
     gene            complement(3243..3947)
                     /locus_tag="PLO65_03885"
     CDS             complement(3243..3947)
                     /locus_tag="PLO65_03885"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRD27422.1"
                     /translation="MRIRLIGLAVATLLAAAAPAAATFAASAPTSWDDLVRVKSRALD
                     SVYLLPGVDFRPYTKVMIDPAEVAFRKGWLRDYNASTSSLAARLDESDAERMAAEART
                     GFDGIVREAFAAAGYEIVDTPGADVLRVSPSVFNLAINAPDVPSAGRVSNWSVEAGEA
                     SVLVEVRDSTTGALLGRAVDRARLDDGRMTRRTEASNRADFEQLFRRWSATMVNGLAQ
                     LKALSPISASGTPARP"
     gene            complement(4008..4715)
                     /locus_tag="PLO65_03890"
     CDS             complement(4008..4715)
                     /locus_tag="PLO65_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27423.1"
                     /translation="MSDPPSDLLPDKVRQGLSRLAALPASVRSIGWLSFLEIAFAVVL
                     SAAGLGSAWSSQEMRLWGGVQAEEYARTSALRGSASRLRAAADARRNIEIGLFSQWLN
                     AKVTGQEALANAYRRRFPPAFSRTFEAWLALRPLENPSAPASPFHMAGTDPDGREAER
                     TEERAEAAFRNAQNANRVSDLFGQVNVIFATAMFISGIGNHFDGRRVRAVLMTVGLLC
                     CVIGFWRMGHLPALFTR"
     gene            complement(4739..5380)
                     /locus_tag="PLO65_03895"
     CDS             complement(4739..5380)
                     /locus_tag="PLO65_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD27424.1"
                     /translation="MNGLRIMVADEQPVVRWGVRQVIEARQGWQLCGEAASDEEAVSV
                     ALTQRPDIAVIGLAPRGGRQLGVIERFARAGLEARVLIFSSSQDDEAIAAGLAAGAQG
                     YVLKTDSLEEMERAIATLASRRSYLSPAVASRMRETRERGDGVAEPKSLTERELEVTR
                     LVVEGLSNRQIASLLGRSVKTIESHRAASMRKAGVRSAAGLVRFAIRHRIAGD"
     gene            complement(5418..6641)
                     /locus_tag="PLO65_03900"
     CDS             complement(5418..6641)
                     /locus_tag="PLO65_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRD27425.1"
                     /translation="MADKTMIALLRRALREAFLRRSRTGHGDHAAKFPSFASVVAALA
                     LLPIQAHGQEAATPAPPSSSEAVEPVPEGDGPRPKLVIAPIPQSDPTLGTGVTVVAMS
                     LYRVGGSQQAWTTGGGAMYTDSRSWGALLLQRAYFRHDTIRVTAIAGYGDFNIDFYGV
                     GADAAARGQSVGLNQKGYGAIVQGLVEVQPNLFVGPRLQYVDLTSRLTTPPIPGLGID
                     PADLTLNAVSVSLGLGSEYDTRDSEYGPRTGTLASGQWLVASPDLGSDFSYSKFQASI
                     NGYHPAGDKGVLAGRVFVCGASDDAPFFDLCLFGSHNDLRGYASGQYRDSAMVAAQME
                     FRRRLFWRIGYVAFAGVGSVAPGFSDLGDARLLPAAGVGLRFEASAEYRLNFSIDAAV
                     GDHSAGVYVYLGEAF"
     gene            complement(6662..7117)
                     /locus_tag="PLO65_03905"
     CDS             complement(6662..7117)
                     /locus_tag="PLO65_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27426.1"
                     /translation="MRFTPRAIGCVTAAVSLVVSGLAPAASSAAPRGAPTSVNPGPRG
                     PGGPGSPGGPGGGNGGRHDGDRINVGNDIDIDIDTDPGWDHDHDHHPVAVGVAVGTAL
                     AIGSRYTVLPPSCTTSYYGGYTYYYCGADWYRAYYAGTTVTYVVVARPY"
     gene            complement(7132..7914)
                     /locus_tag="PLO65_03910"
     CDS             complement(7132..7914)
                     /locus_tag="PLO65_03910"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HRD27427.1"
                     /translation="MKSTHRLLATLLATGAVAAATVPAQSAFAAEATAPAVSPAVDPA
                     ALQALERMSAYLGTLTTFRVVAQTTYDQVYDNGQTLTFDSTITYDVRRPDGFIINMAA
                     DRHVRQIYFDGQSITLFAPNRGLYGTVPAPATIDQTLDLAWQRYGILIPLRDLFRWSA
                     PGGDRKLDLRTGVRVGYARIDGVDCDQYAFQEGGIDWQIWIARGAKPVPVRVVIIDAT
                     DLAHPQFSANLTWTETPAYAADAFTFKPPANSSRIGVEEVEQ"
     gene            complement(7972..>9643)
                     /locus_tag="PLO65_03915"
     CDS             complement(7972..>9643)
                     /locus_tag="PLO65_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD27428.1"
                     /translation="APRPLRRRSAGPSGKPLNVLFIFTDQERYFRRLPPGLSLPAHER
                     LMRTGTTFENHYIGAVMCTSSRSIMLTGLQTADNGMFENADMPWVPDLDPKIPTLGTM
                     LRKAGYYTAYKGKWHLTKSFDQHSPERLFTAEMDAHGFADYASPGDVVGHTLGGYEFD
                     HLIGGSVLTWFRRHGVELSAQGKPWALTTSLVNPHDIMYFNSDGPGAKVQDNGKLLKH
                     VARAPDVPIYQAHWDMPLPATNKEALRSAVRPGAHAEYQEAWDALLGHVPNEADRWSR
                     FNDFYINSIRAVDAQVMNILNELDTLGLSDNTIIVFTADHGEMAGAHGGIRGKGPFAY
                     EESLHVPLMIVHPEVQGGHSTRALTSHIDLVPTLMSLAGAGDKTAEFAGRTLPGRDFS
                     SLLAKRNAGVNELRESVLFAYSGIAANDAQMVKAAADAIVSGKGIASLQAAGVKPDLS
                     KRGTLRSTFDGRYKFTRYFSPMQRHRPGNIDQLYAHNDLELFDLASDPNEARNLAATK
                     GQNDELVLAMSAKLERAIAAEIGVDDGREMPDLNGKDLNWALPMNALD"
BASE COUNT         1577 a         3195 c         3359 g         1512 t
ORIGIN      
        1 cgcccgggcc cgcgcctctg tcagcgccgc gttcgacgcc gcgcgcgacg cggtgaaggc
       61 catgacggcc gtggccggaa tcctcgacgc cggcctgttc aagcggcagt accgcgatca
      121 ccgtctcgaa tggatgatcc gctccggcgg cgtcgaggtc gtattgcggg ggctggaaaa
      181 gggcgacatc cgcttcaatg acgagccggt ctgaccgcgg cggggcaaga aaaatcaccg
      241 gccgagattg cgtcggcttt gatcctgatc aacgataacc taccggcggc gtcgaactct
      301 gccgtcgaac agatcgtccg gcccaagccg gcagaggaaa ccgtccatga ccgacaccgc
      361 caccctttcg cgcgctgcga ccacgaccac cgaacatttc gacgttctga tcgtcggcgc
      421 cgggatttcc ggtgtgggcg gcgcctatca tctcaagacc cagtgcccgg ggaccagcta
      481 cgtcgtgctt gaggggctcg agaacttcgg cggcacctgg tggatgcacc gctacccggg
      541 catccgctcc gacagcgacc tctacacctt cggatatcgg ttcaagccct gggtcggggc
      601 gccaatcgcc agcgccgagg aaatcctgaa atacatgggc gaggtcatcg acgagaacga
      661 cctggcccgg cacatccgct atggacacca gatcacctcg gcgacctggt cgacgtcgga
      721 ccgcaagtgg accattgagg gcgtccgcaa ggacaccggc cagccggtcc gcttcacctg
      781 caacttcctc tggatgtgcc agggctacta ccgccactcc gaaggctaca cgccggagtg
      841 ggagggcatg gacaccttca agggaaagat cgtccatccc cagacctggc cccaggatct
      901 cgactacagg aacaagaagg tcatcgtcat cggctccggc gcgaccgccg cgaccctggt
      961 gccggcgatc gccggcgaca cggcccacac caccctgctg cagcgctcgc cgacctattt
     1021 catcccgggc cgcaacgaga acgagctggc caacaccctg cgcgaactcg agattcccga
     1081 ggaatggatc cacgagatcg tccgtcgcaa ggtgctgttc gaccaggccg ccttcaccaa
     1141 gcgggccgtc gaggagtcag aggcggtcaa gaacgagctg ctggagggcg tgcgcgcctt
     1201 cgtcgggccc gacttcgaca tcgagaagca tttcaccccg aagtacaggc catggcgcca
     1261 gcgcatcgcc ttcgtgcccg acggtgacct gttccagggc gtggtctccg gcaaggcctc
     1321 ggtggtcacc gacgagatcg atcgcttcac cgagaagggc atcctgctca agtcgggcca
     1381 ggagctggag gccgacatca tcgtcacggc gaccggcttc aacctcagcg tcctgggcga
     1441 catcaggttc gtcatcgacg gcaagccgtt gaacttccac gacacggtga cctaccgcgg
     1501 catgatgttc accggcgtgc ccaacatggt ctgggtgttt ggctatttcc gcgccagctg
     1561 gaccctgcgg gccgatctga tcggcgactt cgtcgcccgc ctgctgaatc acatgaagaa
     1621 gaagggggcg accatggtca ccccgacgct tcggccggaa gacagggaca tgccgctcag
     1681 cgactggatg gatccggaga acttcaaccc cggctacctg atgcgcggca tgcacctgct
     1741 gccgaagaag ggtgacaagc ccgaatggca gcacacccag gactactggg ccgagaagga
     1801 cgagattccg gccatcgacc tggacgacgc ggccttcgtc tacaggtgaa acgaaaaggg
     1861 gcggccgagc aggccgcccc ccgccgtcat ctggaacagc ggcctcactg catccggtgc
     1921 aggccacaca gcttgttgcc gtccggatcg cgcagatagg ccagatacat cttgccggtg
     1981 gcgcccgcgc gcaccccggg cgggtcttcg caggtggtcc cgccggcggc caggcccgcg
     2041 gcgtgccagg cgtcggccac ctcgggcgag gaacaggcga agccgatggt gccgccgttg
     2101 ggagccgtgg ccggttcgcc attgatcggc ttcgacaccg agaacacccc ggtcggcgtc
     2161 atgtagaaga tccggtggcc atcgacgaag gccggcggaa cgccgagcgt gccgagcagg
     2221 gcgtcataga agctcttggc ccgctccagg tcattggtgc cgatcatgat atgcgaaaac
     2281 atgtggtcgt cctcccgtgt cggcgccgtt cgattcggcc agccgtgtcg caccgggatg
     2341 tcgcccgctc cccgcggccc gcgcaagcag caaggcgggc cttaccttcg gtcagcggga
     2401 cgatttcctc gccagccccc ggtgacatcg ggccctgggg cgcggcgggc gcccggccat
     2461 tcccgcgggg cgggaatgcg gtcttcggcg gttgacggcc gctagtccag cgggtccaga
     2521 cgggcttgga ggcgagcccc gtccgcgaac acgtcgcgag cggcgcggcg gccgatggcc
     2581 gtgacctggg tggggtcgac gccgaggccg cgcagcaggt ccgccgcaag ctcggcggca
     2641 aaggcctcgg tggcgacggg ccgggtcgac tccagcaggt agagaatgct catctggacg
     2701 atgccgatga tcagcaggat gccgctctgc aggcttggcc cgcggaaacg cccctcttcc
     2761 agcccccgcg ccacgtccgc gaggaccccg atgttcacat ccgccagggg cagggcggtg
     2821 gcgccggcga acagccggac catcgaccgg gcccgcagcg gctcgaccgt cgagaagtgt
     2881 acgaagatgg ccaatgcccg cgcgacgcgt tcggcggggt ccgaaatccc ctcgttcgcc
     2941 gcatcgacca tcgcctcgcc cgcgaggcgc acattgcgtg aaatctcgcg ggccagcgcc
     3001 tccttgtcct cgaagtggtt gtagaagctg ccacgggtga cgccggccgt cttgacgatg
     3061 tcatcgacgc tgacgacctc gatggcatgg ctgatgagga gggcctcgcc cgcacgcaac
     3121 agggcccggc gagttcgcgc ccgccggggg tcgatccgtg gactatgccg ctccatcccc
     3181 gcctccagga gacccgttct aggtgaagtc gacgcccctg aaaaccatct ggcccgatcc
     3241 ggtcagggcc gggccggcgt ccccgacgcg gaaatgggcg acagggcctt caactgggca
     3301 aggccattga ccatcgtcgc cgaccagcgt cggaacagct gctcgaagtc ggcccggttg
     3361 gaggcctcgg tccggcgcgt catccggcca tcgtcaaggc gcgcccgatc gaccgcgcgc
     3421 cccaggagcg cgccggtcgt ggaatcgcgc acctcgacca gcacgctggc ttcgcctgcc
     3481 tcgaccgacc agttggacac ccgtcccgcc gagggcacgt cgggcgcgtt gatcgccagg
     3541 ttgaagacgc tcggcgagac gcgcaggaca tcggcgcccg gggtgtcgac gatctcgtag
     3601 ccggccgcgg cgaacgcctc gcggacgatg ccatcgaagc cggttcgcgc ctcggcggcc
     3661 attcgctcgg cgtcggactc gtcgagacgg gcagcgaggg acgaggtcga ggcgttgtag
     3721 tcccgtagcc agcctttgcg gaacgcgacc tcggcaggat cgatcatgac cttggtgtag
     3781 ggccggaaat cgacgcccgg caggaggtag acagagtcga gggccctgga cttgacgcgg
     3841 acaagatcgt cccagctggt gggcgcggac gccgcgaagg tcgcggccgc cggcgcggcg
     3901 gcggccagga gggtggcgac cgcgagaccg atcaggcgaa tacgcatgga ctgactcctt
     3961 tcaggggttt gggatgtcga cagggaatgt caccgggcga agccgcttca ccgggtgaat
     4021 agcgcgggca ggtggcccat gcgccagaag ccgatcacgc agcagagcag ccccacggtc
     4081 atcagcacgg ccctgacgcg ccggccgtcg aagtgattgc cgatccccga gatgaacatg
     4141 gccgtggcga aaatgacgtt gacctgtcca aacaggtcgc tgacgcgatt ggcgttctgg
     4201 gcgttccgga acgccgcctc cgcgcgctcc tcggtccttt cggcctcgcg accgtcggga
     4261 tctgttccgg ccatgtggaa cgggcttgcc ggcgcgctcg gattctccag ggggcgcagg
     4321 gccagccagg cttcgaaggt tctgctgaag gccggcggaa aacgccgccg gtaggcgttg
     4381 gccagggcct cctgacccgt caccttggcg ttcagccact ggctgaagag gccgatctcg
     4441 atgttgcgac gggcgtccgc cgcggcgcga aggcgggacg cggagccccg tagcgcggat
     4501 gtgcgggcat attcctcggc ctggacgccg ccccacagcc gcatctcctg gctcgaccag
     4561 gccgagccaa ggccggccgc cgacagcacg acggcgaagg cgatctcgag gaagctcagc
     4621 cagccgatcg atctcaccga ggcgggcagg gcggccagcc gcgacaggcc ctggcggacc
     4681 ttgtcaggca gcagatcgga aggcggatca gacatcgaac ggttgcggtt tccggggttc
     4741 agtcgccggc gatccggtgc ctgatggcaa agcggaccag gccggcggcg ctgcgcacgc
     4801 cggccttgcg catcgaggcg gcgcggtggc tctcgatcgt cttgacgctg cgacccagca
     4861 ggctggcgat ctgccggttg ctcaggccct cgacgaccag gcgggtcacc tcaagttcgc
     4921 gctcggtgag gcttttcggc tccgcgacgc catcgccgcg ttcccgggtc tcgcgcatcc
     4981 ggctggcgac ggccggcgac aggtagcttc gccggctggc gagggtcgcg atggcgcgct
     5041 ccatctcctc gaggctgtcg gtcttcagga catagccctg ggcgccggcc gcgagccccg
     5101 cggcgatcgc ctcgtcgtcc tggctcgagg agaaaatcag caccctggcc tccaggccgg
     5161 cccgggcgaa ccgttcgatg accccgagtt gccgtccgcc cctgggcgcg agaccgatca
     5221 cggcgatgtc cgggcgctgc gtgagggcga ccgacacggc ctcctcgtca ctggcggcct
     5281 cgccgcacag ttgccagccc tggcgggctt cgatgacctg cctgaccccc cagcgcacca
     5341 ccggctgttc gtcggcgacc atgatccgca ggccgttcat ggacggccgc cctgcgatcg
     5401 atcggcacgc aggcgcatca gaacgcctcg ccgagataga cgtagacccc ggcggaatgg
     5461 tcgccgacgg ccgcgtcgat gctgaagttc aggcgatatt cagcggaagc ttcaaaccgc
     5521 aggccgacgc cggccgcggg cagcaggcgc gcgtctccca ggtcggaaaa gccgggcgcc
     5581 acgctcccga cgccggcgaa cgccacatag ccgatacgcc agaagagacg ccggcggaat
     5641 tccatctggg cggccaccat ggccgagtcg cggtactggc cgctcgcgta gccccgcagg
     5701 tcgttgtggc tgccgaagag gcagaggtcg aagaagggcg cgtcgtcgct ggcgccgcag
     5761 acgaacaccc ggccggccag gacccccttg tcgcccgcgg gatggtagcc gttgatcgag
     5821 gcctggaact tcgaatagct gaagtccgat ccgagatcgg gcgaggcgac gagccactgt
     5881 cccgaagcca gggttcctgt ccgcggcccg tattcactat cccgtgtgtc gtattcgctg
     5941 ccgagcccca ggctgacgct gacagcattg agagtcaggt ccgcagggtc gatgccgagt
     6001 cccggaatcg gcggggtcgt caggcgggag gtcaggtcga cgtactgcag ccgcggcccg
     6061 acgaacaggt tcggctggac ctccaccagg ccctggacga tcgccccgta gcccttctgg
     6121 ttcaggccga ccgattgacc ccgggcggcc gcatcggcgc cgacgccata gaagtcgatg
     6181 ttgaagtcgc catagccggc gatcgccgtg acccggatcg tgtcatgccg gaaataggcg
     6241 cgctgcagta acagggcgcc ccagctgcgg gagtccgtgt acatggcgcc gccgccggtg
     6301 gtccaggcct gctgggagcc gcccacgcgg tagagcgaca tggccacgac cgtcacgccc
     6361 gtgcccagag tcgggtcgga ctgggggatg ggggcgatca ccagtttcgg tcgcggtcca
     6421 tcaccctcgg ggacgggttc gacggcctcg gaggaggagg gaggcgccgg cgtcgcggcc
     6481 tcctggccat gggcctggat cggcagcagc gccagggcgg cgaccacgct cgcgaaactg
     6541 gggaatttcg cggcgtgatc gccatgaccc gtccgggagc gccggaggaa agcctcccgg
     6601 agagccctcc tgagcagggc gatcatcgtc ttgtcggcca ccgccctgcg tcctgcgcag
     6661 cctagtaggg gcgcgcgacg acgacgtagg tgacggtcgt cccggcgtag taggcccggt
     6721 accagtcggc cccgcagtaa tagtaggtgt atccgccgta gtaggacgtc gtgcaggacg
     6781 gcgggagcac ggtatatcgc gagccgatcg ccagagccgt tccgaccgcg acgcccaccg
     6841 cgacgggatg gtggtcatga tcatgatccc agccgggatc ggtatcgata tcgatgtcga
     6901 tatcgttgcc gacattgatc cggtcgccgt catgccggcc cccgttgccg ccgcccgggc
     6961 cgcccggact gccaggtccg ccgggaccgc gaggacccgg gttgacgctg gtcggcgcgc
     7021 cgcgaggggc ggccgagctc gccgccggcg cgaggccgct gacaaccagg gagaccgccg
     7081 ctgttacgca gccgatcgcg cggggagtga agcgcatctg gtttttcatc gtcattgttc
     7141 gacctcttcg acgccgatcc ggctcgaatt cgccggcggc ttgaacgtga atgcatccgc
     7201 ggcgtaggcg ggggtctcgg tccaggtcag gtttgccgag aactgcgggt gggccaggtc
     7261 ggtcgcgtcg atgatgacca cccggaccgg caccggcttg gcgccgcgcg cgatccagat
     7321 ctgccagtcg atgccgcctt cctggaaggc gtactggtcg caatcgacgc catcgatccg
     7381 ggcgtagccg acccggacgc ccgtcctcag gtcaagcttc cggtcgcccc cgggcgcgct
     7441 ccagcggaac aggtcacgga gcggaatgag gatgccgtac cgctgccacg cgaggtcgag
     7501 ggtctggtcg atcgtggccg gcgccggcac cgtcccgtaa aggccccggt tgggagcgaa
     7561 cagggtgatc gactgaccgt cgaaatagat ctgccgcaca tggcggtcgg cggccatgtt
     7621 gatgatgaaa ccatccggac ggcggacatc gtaggtgatg gtgctgtcga aggtcagggt
     7681 ctggccgttg tcgtagacct ggtcataggt ggtctgggcg actacccgga acgtcgtcag
     7741 cgtcccaagg taggcgctca tgcgctcgag cgcctggagc gcggcgggat cgacggccgg
     7801 cgagacggcg ggagccgtcg cctcggccgc gaaggcggac tgggccggga cggtcgcggc
     7861 cgcgacggcg ccggtggcca acagcgtggc caggagtcgg tgggtggatt tcacggtagt
     7921 cgcctttctc ggtattcgtc ggatcggatc cagggccgat gggccggccg gtcagtccag
     7981 ggcgttcatg ggcagggccc agttcaggtc cttgccgttg aggtccggca tctcgcggcc
     8041 gtcatcgacg ccgatttcgg cggcgatggc gcgctcgagc ttcgcgctca tcgcgagcac
     8101 cagctcgtcg ttctgaccct tggtcgccgc gaggttcctg gcctcgttcg ggtcgctggc
     8161 caggtcgaac agctcaaggt cgttgtgggc gtagagctga tcgatgttcc cgggccggtg
     8221 gcgctgcatc ggcgagaaat agcgggtgaa cttgtaccgg ccgtcgaagg tcgacctcag
     8281 ggtgccacgc ttgctcaagt ccggcttgac cccggcggcc tggagggacg cgatgccctt
     8341 gcccgagaca atggcgtcgg ccgcggcctt gaccatctgg gcgtcgttgg cggcgatacc
     8401 gctgtaggcg aacagcaccg actcgcgcag ctcgttcacg ccggcgttgc gcttggccag
     8461 cagggacgag aagtcccgtc cgggcagggt gcggccggcg aattcggccg tcttgtcgcc
     8521 ggccccggcc agggacatca gcgtcggaac caggtcgata tggctggtca gggcccgggt
     8581 cgaatgccct ccctgcacct cgggatggac gatcatcagc gggacgtgca gcgactcctc
     8641 gtaggcgaac ggtcccttgc cccggatgcc gccgtgggcg ccggccatct cgccatggtc
     8701 ggccgtgaag acgatgatcg tgttgtccga caggccgagc gtatccagct cgttgagaat
     8761 gttcatcacc tgggcgtcaa ccgcccggat gctgttgatg tagaagtcgt tgaatcgcga
     8821 ccagcggtcc gcctcgttgg gcacatggcc gagcagcgcg tcccaggctt cctggtattc
     8881 ggcgtgggcg cccggccgca cggccgagcg cagcgcttcc ttgttggtgg cgggcagggg
     8941 catgtcccag tgcgcctggt agatcggcac atccggcgcg cgggccacat gcttgagcag
     9001 cttgccgttg tcctggactt tcgctcccgg cccgtccgaa ttgaagtaca tgatgtcgtg
     9061 cgggttcacg aggctggtgg tcagggccca gggcttgccc tgggcgctga gctcgacccc
     9121 gtgccgccgg aaccaggtca gcacgctgcc gccgatcagg tggtcaaact cgtagccgcc
     9181 gagcgtgtgg cccacgacgt cgcccggcga ggcgtagtcg gcgaagccgt gagcatccat
     9241 ttcggccgtg aacaggcgtt cgggcgagtg ctggtcgaag ctcttggtga gatgccactt
     9301 gcccttgtag gccgtgtagt agccggcctt gcgcaacatg gtcccgagtg tcgggatctt
     9361 cgggtcgaga tcgggcaccc agggcatgtc cgcgttttcg aacatcccgt tgtcggccgt
     9421 ctgaagcccg gtgagcatga tcgagcgcga cgaggtgcac atcactgcgc cgatgtagtg
     9481 gttctcgaag gtggtgcccg tacgcatcag acgctcgtgc gccggcagcg acagccccgg
     9541 gggcaggcgg cggaaatatc gctcctggtc ggtgaagatg aagaggacgt tcaggggctt
     9601 gccggaaggc ccggcggacc ggcggcgcag cggccgcggc gcc
//
LOCUS       DAORNX010000056         9580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5261_length_9580_cov_9.733858, whole genome shotgun sequence.
ACCESSION   DAORNX010000056 DAORNX010000000
VERSION     DAORNX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5261_length_9580_cov_9.733858"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLO65_03920"
     CDS             complement(<1..685)
                     /locus_tag="PLO65_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRD27429.1"
                     /translation="MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTNRQGVEYAWFNNV
                     ETKPGVGYPRDWENQKKWNGGWRRKANGKIEPRMGAKWRILSKIFANPDLPEIDDYYE
                     PFDFDYGHLQTAPEMKHFPTARPRSKITGQRMEKIEWGPNWEEILGGEFAKRSADSNF
                     EGVEKEIYGQFEQTFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRG
                     WRMCVSACPY"
     gene            complement(682..4413)
                     /locus_tag="PLO65_03925"
     CDS             complement(682..4413)
                     /locus_tag="PLO65_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRD27430.1"
                     /translation="MSHTLDRLAFFTRKTELFSDGHGVMNDDDRAWEEAYRSRWRHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPELPNHEPRGCARGASYSWY
                     LYSANRVKYPLVRSRLLRLWREARKTLAPVAAWASIQGDPAARKSYTSMRGSGGFVRA
                     TWDEVTEIVAAANAHTVKRWGPDRVFGFSPIPAMSMVSYAAGARYLQLLGGACGSFYD
                     WYCDLPPASPQTWGEQTDVAESADWYNSSFMLLWGSNVPQTRTPDAHFYTEARYRGAK
                     SVVICPDYSEASKFSDLWLPVKQGTDAALAMAFGHVILKEFHIDRQVPYFRDYLRKYS
                     DLPMLVRLVPQEGGHVPERLVRASDFVGDLDQANNPDWKTVAIDEASGEIVVPNGSIG
                     FRWGEDGRWNLEERDAAGRETTLRLGLKDAHDEVAGVRFPYFANTASNGFASTDHPDV
                     LTRNVPVRRVMLKDGEALVACVYDLFLANYGVDQGFGGDCMPASHDDPQPYSPAWAEA
                     ITTVPREQIIAVARGFATNAEKTGGKSMVIIGAAMNHWFHMDMNYRGVINMLVMCGCV
                     GQSGGGWAHYVGQEKLRPQTGWAPLAFATDWIRPPRQQNSTSFFYAHADQWRYETVGV
                     DELLSPTAPDGPWKASMIDFNVRAERMGWLPSTPALKTNPLEVARAAAAAGADPKAYT
                     VAALKDRSVALSCMDPDDPANWPRNMFVWRSNLLGSSGKGHEYFLKHLLGAAHGVQGK
                     DLGEAGKVKPVDVVWHDEAPEGKLDLLVTLDFRMSTTSVYSDIVLPTATWYEKNDLNT
                     SDMHPFIHPLSAAVDPAWESKSDWEIFKAIARKFSEVAPEVLGVEQDVVLTPIQHDSA
                     AEMAQPFEVKDWYLGECEAIPGRTMPQITIVERDYPNLYKQFTALGPLLDKAGNGGKG
                     LAWKTGHEVDALRDLNGAVSDAGLTEGMPSIDTDIDACETILMLAPETNGEVAVKAWT
                     ALEKQTGREHTHLALPKEDEKIRFRDLLAQPRKIISSPTWSGIESEKVCYNAGYTNVH
                     ELIPWRTLTGRQQLYQDHLWMRAFGEALCVYKPPIDLKTTHVKGLRPNGETEIVLNFI
                     TPHQKWGIHSTYSDNLLMLTLNRGGPVVWISETDARKAGIVDNDWIEVFNANGALTAR
                     AVVSQRIREGTTFMYHAQEKIVNTPGSEITGRRGGIHNSVTRAVLKPTHMIGGYAQLA
                     YGFNYYGTVGSNRDEFIVLRKMKNVDWLEQPLEAREFGQ"
     gene            complement(4410..5834)
                     /locus_tag="PLO65_03930"
     CDS             complement(4410..5834)
                     /locus_tag="PLO65_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287880.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRD27431.1"
                     /translation="MASAPTPKHVLTDWRPEDPAFWASTGKAVATRNLWISVPNLLLA
                     FSVWMVWSMVIAKLKLIGFTFTTEQLFWLAALPALSGATLRIFYSFIVPVFGGRLWTV
                     LSTLSLLIPAIGIGVAVQNPHTPYIAFVALALLCGFGGGNFASSMSNISFFFPKAEKG
                     NALAINAGFGNLGVSVMQFLIPLVIAMGAFAPVVGAPQIAVEKGVESQIWLQNAGFIW
                     VPFILAAAAAAWIGMNDISSARASFATQAVVFRRRHNWIMCYLYVGTFGSFIGFSAAF
                     PLLAKTLFPAVMVLKIAFLGPLVGALSRALTGWVSDRWGGQHVTHWVFLALAAGVLGV
                     LYSVGATGAAPSFPLFFAAFLWLFFWTGVGNASTFQMIPAIVRADMPRLMPRADLATR
                     LKASEMEAAAIVGFSSAIGAYGGFFIPMAFGDSLKATGGPATALLIFFVFYLSCVAVN
                     WAFYARRKSLLNTPSLTLAPEPAA"
     gene            complement(5851..7224)
                     /locus_tag="PLO65_03935"
     CDS             complement(5851..7224)
                     /locus_tag="PLO65_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRD27432.1"
                     /translation="MVAKMGGGGSKGAAGAGTALWASTIAFTACFAVWTIFSIIGVQI
                     KRDLGLSEAQFGLLAGTPILTGSLVRVFLGVWTDQYGGRLVNLLVMLAAALATFLLSH
                     AQTYPQFLLAALGVGMAGGSFAVGVAYVSKFFPKERQGTALGIFGAGNVGAAVTKFAA
                     PFVMIAFGWEAVAQIWAGSLAVIAVAFFLLTRDDPALVERRRTGAKAATTAMQFTPLM
                     KLQVWRFSLYYFFVFGAFVALALWLPHYLVGVYGLNIKVAGMVAAAYSIPGSLFRVFG
                     GWLSDRIGARRVMYITFSVSAACTFLLSYPPTSYVVAGIHGPISFQLATGLIPFIGLL
                     FCLGFAMSLGKAAVYKHIPVYYPDTIGSVGGLVGMVGGLGGFILPIAFGLLNDLTGVW
                     TSCFMLLFVLVASALTWMHFAIRRMERARTPQLAHLPQLPEMAGLHPDPLSGLAADAP
                     APVAATR"
     gene            7518..8291
                     /locus_tag="PLO65_03940"
     CDS             7518..8291
                     /locus_tag="PLO65_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD27433.1"
                     /translation="MRSVDIERVRALPLFSRAAPRTFDGLIAGAFLQKFPAGTTLLME
                     GDPVDFLYVLIEGLAELQGTWNDKETTLAVLRPVSTFILAAVVLDADALMTARTIERS
                     DILMISGEAVRRAMKEDAAFAVAVAEELSGCYRGLVRTVKNQKLRGASERLANYLVAE
                     QISQGGGPSFVLPHEKRVLASLLGMTPENLSRAFANLADYGVAVNGPEVSILRPAVLD
                     RLAKPDRLIDSHLQPSAARAGMADRECRASAAGKSAAWN"
     gene            complement(8292..9275)
                     /gene="moaA"
                     /locus_tag="PLO65_03945"
     CDS             complement(8292..9275)
                     /gene="moaA"
                     /locus_tag="PLO65_03945"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRD27434.1"
                     /translation="MTRLIDSLGRRFQYLRLSITEVCNFRCTYCLPNGYQKTQPASFL
                     TVAEIGRLAEVFAQLGVGKIRLTGGEPSVRRDVGEIIRRVRATPGIDKVAITTNGWNL
                     ARNINDWHAAGLTNLNVSVDSLDREMFHRITGHDRLPAVMAGLDRALELGVPSVKINA
                     VLLKDAAGPEVFERFAAFVRTRPVAVRFIELMRTGDNADYFARQHVGGPVLRDWLEAG
                     GWTPRARAHDDGPATEFTHPDHAGRFGLIAPYGAGFCDGCNRLRITARGKLRLCLFGE
                     GGIDLRDLLSEDSDAEALAGRIVTALGGKAAGHRLAEQITGDTLQLAQLGG"
     gene            complement(9359..>9580)
                     /locus_tag="PLO65_03950"
     CDS             complement(9359..>9580)
                     /locus_tag="PLO65_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRD27435.1"
                     /translation="ATLGHTGRPRQSDRATTLFYVGANLAAVLRVAAAFAVVQREPLL
                     VAAGSLWVLAFAAFVLRYGPMLVRPRLAS"
BASE COUNT         1586 a         3139 c         3361 g         1494 t
ORIGIN      
        1 tgtaggggca ggccgagacg cacatccgcc agccgcggca cttgtcctgg tcgatcagga
       61 cgatgccgtc ctcctcgcgc ttgtagatcg cccccgaggg gcaggccgcc acgcaggtgg
      121 ggttgaggca gtgctcgcac agcctcggca ggtacatcat gaaggtctgt tcgaactggc
      181 cgtagatctc cttctcgacg ccctcgaagt tggagtccgc ggaccgcttg gcgaactcgc
      241 cgcccaggat ttcctcccag ttgggacccc attcgatctt ctccatccgc tggccggtga
      301 tcttcgagcg cggccgggcg gtcgggaaat gcttcatctc cggcgcggtc tgcaggtggc
      361 cgtagtcgaa atcgaacggc tcgtagtagt cgtcgatctc gggcaggtcg ggattggcga
      421 agatcttcga caggatgcgc cacttggcgc ccatgcgcgg ctcgatcttg ccgttggcct
      481 tgcgccgcca gccgccgttc catttcttct ggttctccca gtccctggga tagccgacgc
      541 cgggcttggt ctcgacgttg ttgaaccagg cgtactcaac gccctgacgg ttggtccaga
      601 cgttcttgca ggtgaccgaa caggtgtgac agccgatgca cttgtcgaga ttcagcacca
      661 tgccgatctg ggcgcgaacc ttcattgacc gaactcccgg gcttcgagcg gctgttccag
      721 ccagtcgacg ttcttcattt tgcgaaggac gatgaactcg tcccggttgg agccgacggt
      781 gccgtagtag ttgaagccgt aggcgagttg ggcgtagccg ccgatcatgt gggtcggctt
      841 gagcaccgcg cgggtgaccg agttgtggat gccgccgcgc cgcccggtga tctccgagcc
      901 cggcgtgttc acgatctttt cctgcgcgtg gtacatgaag gtcgtgcctt cacggatgcg
      961 ctgggaaacc accgcccggg ccgtcagggc cccgttggcg ttgaagacct cgatccagtc
     1021 gttgtcgacg atcccggcct tgcgggcgtc cgtctcggaa atccagacca ccggcccgcc
     1081 gcggttgagc gtcagcatca gcaggttgtc ggaataggtc gagtggatgc cccacttctg
     1141 gtgcggggtg atgaagttga gcacgatctc ggtttcgccg ttgggtctga gacccttcac
     1201 gtgcgtcgtc ttcaggtcga tcggcggctt gtagacgcac agcgcctcac cgaaggcccg
     1261 catccacagg tggtcctgat agagctgctg gcgccccgtg agcgtccgcc aggggatcag
     1321 ctcatgcacg ttggtgtagc cggcgttgta gcagaccttc tcgctctcga tgcccgacca
     1381 ggtcggcgag gagatgatct tgcgcggctg ggccagcagg tcgcggaagc ggatcttctc
     1441 gtcttccttg ggcagggcca gatgggtgtg ctcgcgtccg gtctgcttct ccagcgccgt
     1501 ccaggccttg accgccacct cgccgttggt ttccggcgcc agcatcagga tggtctcgca
     1561 ggcgtcgatg tcggtatcga tgcttggcat gccctccgtg aggccggcgt cggacaccgc
     1621 gccgttcagg tcgcgcaggg cgtcgacctc gtgaccggtc ttccaggcga ggcccttgcc
     1681 gccgttgccg gccttgtcga gcagcgggcc gagcgcggtg aactgcttgt agaggttggg
     1741 atagtcgcgc tcgacgatgg tgatctgggg catcgtcctg cccgggatcg cctcgcattc
     1801 gccgaggtac cagtccttga cctcgaacgg ctgggccatc tcggcggcgc tgtcgtgctg
     1861 gatcggggtc aggacgacgt cctgctcgac ccccaggacc tcaggcgcca cctcggagaa
     1921 cttccgggcg atggccttga agatctccca gtccgacttc gactcccagg ccgggtccac
     1981 ggccgccgac agcgggtgga tgaacgggtg catgtcggag gtgttgaggt cgttcttctc
     2041 gtaccaggtg gccgtcggca ggacgatgtc ggagtagacc gaagtggtcg acatgcggaa
     2101 gtcgagcgtc accagcaggt ccagttttcc ttccggcgcc tcgtcatgcc agaccacgtc
     2161 gaccggcttg accttgccgg cctcgcccag gtccttgccc tggacgccgt gcgcggcgcc
     2221 gagcaggtgc ttgaggaaat actcgtggcc cttgccggac gagccgagca ggttggagcg
     2281 ccagacgaac atgttgcgcg gccagttggc gggatcgtcg gggtccatgc aggacagtgc
     2341 gaccgagcgg tccttgaggg ccgcgacggt gtaggccttc ggatcggcgc cggccgcggc
     2401 ggcggccctg gccacctcaa gcggattggt cttcagcgcc ggcgtcgagg gcagccagcc
     2461 catccgctcg gcgcggacat tgaagtcgat catcgaggcc ttccacggcc cgtccggcgc
     2521 cgtcggcgac agcagttcgt cgacgccgac cgtctcgtag cgccactggt cggcgtgggc
     2581 gtagaagaac gaggtcgagt tctgctgccg gggcggacgg atccagtcgg tggcgaaggc
     2641 cagcggggcc cagccggtct gcggccgcag cttctcctgg ccgacatagt gggcccagcc
     2701 gccgcccgac tggccgacgc agccgcacat caccagcatg ttgatgacgc cgcggtagtt
     2761 catgtccatg tggaaccagt ggttcatcgc cgcgccgatg atcaccatcg acttgccgcc
     2821 cgtcttttcg gcgttggtgg cgaagccccg ggccaccgcg atgatctgct cgcgcggcac
     2881 ggtggtgatc gcttcggccc aggccggcga atagggctgc ggatcgtcgt gggacgccgg
     2941 catgcagtcc ccgccgaagc cctggtcgac gccgtagttg gccaggaaca ggtcgtagac
     3001 gcaggcgacc agcgcctcgc cgtccttcag catcacccgg cggaccggca cattgcgggt
     3061 cagcacgtcg ggatgatcgg tcgaggcgaa gccgttgctg gcggtgttgg cgaaataggg
     3121 gaagcggacg ccggccacct cgtcatgggc gtccttgagg ccgagccgca gcgtggtctc
     3181 acggcccgcg gcgtctcgct cctcgaggtt ccagcggcca tcctcgcccc agcggaagcc
     3241 gatcgagccg ttcggcacga cgatctcgcc gctggcctcg tcgatggcca cggtcttcca
     3301 gtcggggttg ttggcctggt cgagatcgcc gacgaaatcg gaggcgcgga ccagccgctc
     3361 cggcacgtgg ccgccctcct gcggcaccag ccgcaccagc atcggcaggt cgctgtactt
     3421 gcgcaggtag tcccggaagt agggaacctg gcgatcgatg tggaactcct tgaggatcac
     3481 atggccgaag gccatggcca gggccgcgtc cgtcccctgt ttcaccggca gccacaggtc
     3541 ggagaacttc gaggcctcgg agtagtcggg gcagatgacc accgacttgg ccccgcggta
     3601 gcgggcctcg gtgtagaaat gggcgtccgg cgtgcgggtc tgcgggacgt tggagcccca
     3661 cagcagcatg aagctggaat tgtaccagtc ggcgctttcg gcgacgtcgg tctgctcgcc
     3721 ccacgtctgc ggcgaggccg gcggcaggtc gcagtaccag tcgtagaacg agccgcaggc
     3781 cccgccgagc agctgcagat agcgcgcgcc ggcggcgtag gagaccatcg acatggccgg
     3841 gatcggcgag aagccgaaca cccggtcggg gccccatcgc ttgacggtgt gggcgttggc
     3901 cgcggcgacg atctcggtga cctcgtccca ggtcgcgcgc acgaagccgc ccgagccgcg
     3961 catgctggtg taggacttgc gcgcggccgg atcgccctgg atcgaggccc aggccgccac
     4021 cggcgccagg gtcttgcgcg cctcgcgcca cagccgcagc aggcgcgagc ggaccagcgg
     4081 gtatttcacc cggttcgccg aatagaggta ccagctgtag ctggcgccgc gggcgcagcc
     4141 gcgcggctcg tggttgggca gctccggccg cgtgcgcggg tagtcggtct gctgcgtctc
     4201 ccaggtgacg atcccgccct tgacgtagat cttccacgaa caggagccgg tgcagttcac
     4261 gccgtgggtc gagcggacaa tcttgtcgtg gcgccagcgg gaccggtagg cctcctccca
     4321 ggcgcggtcg tcgtcgttca tcaccccgtg gccgtcggag aacagctcgg tcttgcgcgt
     4381 gaagaacgcg aggcggtcga gggtatggct catgccgcgg gctccggggc gagggtcagc
     4441 gagggggtgt tcagcagcga cttgcgccgg gcgtagaagg cccagttcac cgccacgcag
     4501 ctgaggtaga agacgaagaa gatcagcagg gccgtggccg ggccgcccgt ggccttgagc
     4561 gagtcgccga aggccatcgg gatgaagaag ccgccgtagg cgccgatggc cgaactgaag
     4621 ccgacgatcg ccgccgcctc catctccgag gccttgaggc gggtcgccag gtcggccctg
     4681 ggcatcagcc ggggcatgtc ggcgcggacg atcgcgggga tcatctggaa ggtcgaggcg
     4741 ttgcccacgc cggtccagaa gaacagccag aggaaggcgg cgaagaacag cgggaaggac
     4801 ggcgccgcgc cggtcgcccc gacgctgtag agcacgccca gcacgcccgc cgcgagggcg
     4861 aggaataccc agtgggtgac gtgctggccg ccccatcggt cggacaccca gccggtcagg
     4921 gcgcgcgaca gggcgccgac cagcgggccg aggaaggcga tcttgagcac catcacggcc
     4981 gggaacagcg tcttggccag cagcgggaag gccgccgaga agccgatgaa cgagccgaag
     5041 gtgccgacgt agagatagca catgatccag ttgtgcctac gccggaacac cacggcctgg
     5101 gtcgcgaacg aggccctggc cgagctgatg tcgttcatgc cgatccaggc ggcggcggcg
     5161 gcggcgagga tgaagggaac ccagatgaag ccggcgttct gcagccagat ctggctctcg
     5221 acgcccttct cgacggcgat ctgcggcgcg ccgaccacgg gggcgaaggc gcccatggcg
     5281 atgaccagcg ggatcaggaa ctgcatcacg ctgacgccga ggttgccgaa gccggcattg
     5341 atggccaggg cgttgccctt ctcggccttg gggaagaaga acgagatgtt cgacatcgac
     5401 gaggcgaagt tgccgccgcc gaagccgcac agcagggcca acgccacaaa ggcgatgtag
     5461 ggcgtgtgcg ggttctgcac ggccacgccg atgccgatcg cggggatcag cagcgagagg
     5521 gtcgagagca ccgtccacag tcgcccgccg aacaccggca cgatgaagct gtagaatatg
     5581 cgcagcgtgg cgcccgacag ggccggcagg gcggccagcc agaacagctg ctcggtggtg
     5641 aaggtgaagc cgatcagctt cagcttggcg atgaccatcg accagaccat ccagacggag
     5701 aaggccagca gcaggttggg caccgagatc cacaggttgc gggtggcgac ggccttgccg
     5761 gtcgaggccc agaacgccgg gtcctcgggt cgccagtcgg tcaggacatg tttgggggtc
     5821 ggggcggaag ccatcgggcg ctcctggggg tcagcgggtc gccgcgaccg gcgcgggcgc
     5881 gtcggcggcg agtccggaaa ggggatcggg gtgaaggccg gccatttccg gcagctgcgg
     5941 caggtgggcg agctggggcg tgcgcgcccg ctccatccgg cggatggcga agtgcatcca
     6001 ggtcagggcc gaggcgacca gcacgaacag cagcatgaag cagctggtcc agacgccggt
     6061 caggtcgttg agcaggccga aggcgatcgg caggatgaag ccgcccaggc cgccgaccat
     6121 gccgaccagc ccgccgaccg aaccgatggt gtcggggtag tagaccggga tgtgcttgta
     6181 gaccgccgcc ttgcccaggc tcatcgcgaa cccgaggcag aacaacaggc cgatgaacgg
     6241 gatcaggccc gtggccagct ggaagctgat cggcccgtgg atgcccgcca cgacatagct
     6301 ggtcggcggg taggagagca ggaaggtgca ggcggcgctg acgctgaagg tgatgtacat
     6361 cacccgccgc gcgccgatcc tgtccgacag ccagccgccg aacacgcgga acagcgaacc
     6421 ggggatcgag taggccgcgg cgaccatgcc ggcgaccttg atgttcaggc catagacccc
     6481 gaccagatag tgcggcagcc acagggccag ggcgacgaac gccccgaaga cgaagaagta
     6541 gtagagcgag aagcgccaga cctgcagctt catcagcggc gtgaactgca tggccgtggt
     6601 cgcggctttc gcgcccgtgc gacggcgctc gaccagcgcc gggtcatccc gggtcagcag
     6661 gaagaaggcg acggcgatca cggccagcga tcccgcccag atctgggcca cggcttccca
     6721 gccaaaggcg atcatcacga acggcgcggc gaacttggtc accgcggccc cgacgttgcc
     6781 ggccccgaag atgcccaggg ccgtgccctg ccgttccttc gggaagaact tcgagacgta
     6841 ggcgacgccg accgcgaacg agccgccggc catgcccacg cccagcgcgg ccagcagaaa
     6901 ctgcggatag gtctgggcgt gcgacagcag gaaggtagcg agggccgccg ccagcatgac
     6961 cagcaggttg accaggcggc cgccgtactg gtcggtccag acgccgagga acacccgcac
     7021 cagcgagccg gtcaggatcg gcgtgccggc cagcaggccg aactgcgcct cgctcaggcc
     7081 caggtcccgc ttgatctgca cgccgatgat cgagaagatc gtccacaccg cgaaacaggc
     7141 ggtgaaggcg atggtgctcg cccacagcgc cgttccggcc ccagccgcgc ccttcgaacc
     7201 gcccccgccc atcttcgcaa ccatgtatcc gcgtcggcga tcatcgccca ctgacgcggt
     7261 gtttctgctg catccgtgcg ggcgaataat tgatccgcgt caaagatgaa acgaaaacac
     7321 ggcgaatatc gccgcgtata aacttgctca aatttccgtt tgactgaaaa gtattgcgca
     7381 gttgtcggat gtttacttca cgattgatcg ttatcaagcg gggaatgcgc gcccgtcagg
     7441 agttgatcca ggtcaaggcg cggggaggcc tgagtcggcg aactggcggt ccactggcgg
     7501 ggagattgcg catgcccatg cggagcgtcg acatcgagcg ggtcagggcc ctgcccctgt
     7561 tctccagggc ggccccgagg acgttcgacg gcctgatcgc cggcgccttc ctgcagaagt
     7621 ttcccgccgg cacgaccctg ctcatggaag gcgacccggt cgacttcctc tacgtactga
     7681 tcgagggcct ggccgaactc cagggcacct ggaacgacaa ggagaccacc ctcgccgtcc
     7741 tgcggccggt ctcgaccttc attctcgccg ccgtcgtact cgacgccgac gccctgatga
     7801 ccgcccgcac gatcgaacgg tccgacatcc tgatgatctc cggcgaggcc gtgcgccgcg
     7861 cgatgaagga ggacgccgcc ttcgccgtgg ccgtcgccga ggaactgtcg ggctgctacc
     7921 gcggcctggt ccggacggtg aagaaccaga agctgcgcgg cgcgtccgaa cggctggcca
     7981 actatctcgt cgccgaacag atcagccagg gcggcgggcc gtccttcgtg ctgccgcacg
     8041 agaagcgggt gctggcctcc ctgctgggca tgacccccga gaacctgtcg cgcgccttcg
     8101 ccaacctggc cgactatggc gtggcggtga acgggcccga ggtgtcgatc ctgcgcccgg
     8161 ccgtgctcga ccgcctggcc aagcccgatc ggctgatcga cagtcacctg caaccctccg
     8221 ccgcccgcgc cggcatggcc gaccgcgaat gccgcgccag cgccgccggc aagagcgcgg
     8281 cctggaactg atcagccgcc cagctgggcc aactgcagcg tgtcgccagt gatctgttcg
     8341 gccagccggt gtccggccgc cttgccgccc agcgccgtga cgatgcgccc ggccagcgcc
     8401 tcggcgtcgc tgtcctccga cagcaggtca cgcaggtcga tgccgccctc gccgaacagg
     8461 cacagcctca gcttgccgcg ggccgtgatc cgcagcctgt tgcagccgtc gcagaagccc
     8521 gcgccgtagg gggcgatcag gccgaaacgg ccggcatggt cgggatgggt gaactcggtg
     8581 gccgggccgt cgtcatgcgc gcgcgcgcgg ggggtccagc cgccggcctc aagccagtcg
     8641 cgcagcaccg gcccgccgac gtgctggcga gcgaaatagt cggcgttgtc gccggtccgc
     8701 atcagctcga tgaaccgcac ggccacgggt cgcgtgcgca cgaacgcggc gaagcgttcg
     8761 aacacttccg gtccggccgc gtccttcagc agcacggcat tgatcttcac cgacggcacg
     8821 ccgagctcaa gcgcccggtc cagtccggcc atcacggccg gcagccggtc gtggccggtg
     8881 atccggtgga acatctcccg gtcgagcgag tcgacgctga cattgaggtt ggtcaggccg
     8941 gcggcgtgcc agtcgttgat gttccgcgcc aggttccagc cgttggtggt gatggcgacc
     9001 ttgtcgatcc cgggtgtcgc gcgcacgcgg cggatgatct cgccgacgtc gcggcgcacg
     9061 gacggctcgc cgccggtcag gcggatcttg cccaccccca gctgcgcgaa gacctcggcc
     9121 agccgtccga tctcggcgac cgtcaggaac gaggccggct gggtcttctg gtagccgttg
     9181 ggcaggcagt aggtgcagcg gaagttgcac acctcggtga tcgagagccg caggtactgg
     9241 aaccgccggc cgagactgtc gatgaggcgg gtcatgggcg cgggctcgcg gggatcaccc
     9301 ccgacatagg gcgccgggcc caggccacct tgatccttct caaaggcctg gatgcgcatc
     9361 agctggccag ccgcgggcgg acgagcatcg ggccgtagcg caggacgaag gccgcgaagg
     9421 ctagcaccca gaggctcccg gccgcgacca acagcggctc gcgctgaacg accgcgaagg
     9481 ccgcggcgac ccgcaggacc gccgccaggt tggccccgac atagaacagg gtggtggcac
     9541 ggtcggactg gcggggacga cccgtatggc cgagcgtcgc
//
LOCUS       DAORNX010000057         9430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5381_length_9430_cov_8.856853, whole genome shotgun sequence.
ACCESSION   DAORNX010000057 DAORNX010000000
VERSION     DAORNX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_9430_cov_8.856853"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLO65_03955"
     CDS             complement(<1..985)
                     /locus_tag="PLO65_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRD27436.1"
                     /translation="MFDIKRKTIEWGGRPLTIETGRMARQADGAVVVTYGDTTVLAAV
                     TFAKSPKPGQDFFPLTVNYQEKFYAAGKIPGSFPRRETAPSQKETLTSRLIDRPIRPL
                     FVKGFKNEVLVVATVLSHDLENDPDIVAMIGASAALVISGAPFMGPIAAARVGYVDGE
                     YVLNPTLDQMKETKMDLVVAGTADAVMMVESEIQELSEEVVLGGVSFAHKGMQPVIDA
                     IIELAEHAAKEPFEFETEDTDALKAKMKDLVGADIAAGYKITRKGERHDAIGAAKAKV
                     MTTLGKSETNPDGYDVNKLAGVFKELEADVVRRGILDTGIRIDGRTVDKVRQ"
     gene            complement(1292..1561)
                     /gene="rpsO"
                     /locus_tag="PLO65_03960"
     CDS             complement(1292..1561)
                     /gene="rpsO"
                     /locus_tag="PLO65_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRD27437.1"
                     /translation="MSITLERKAALIAEHGRTAGDTGSAEVQVAILSERIANLTEHFK
                     THKKDNHSRRGLLKLVSQRRGLLDHLKTSDESRYTAIIEKLGLRR"
     gene            complement(1578..2510)
                     /gene="truB"
                     /locus_tag="PLO65_03965"
     CDS             complement(1578..2510)
                     /gene="truB"
                     /locus_tag="PLO65_03965"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRD27438.1"
                     /translation="MARRKKGEAISGWVCLDKPYDFGSTQAVGKVRWLFRAQKAGHAG
                     TLDPLATGVLPIALGEATKTVPFMMDADKAYRFTIEWGRSTATLDREGATTATSDARP
                     TPEQVEAALPAFVGEIQQVPPDYSAVKVDGERAYDLARAGEDFELRARTIKVHALRVS
                     AVPDADHIELEMECGKGAYVRAIVRDLAEALGACGHVSQLRRTRVGPFGEDRAITLEK
                     LEDLSHKAALPEVLLPVETALDDIPALAVTAEDAFRLAQGRPIVLVPRQVETLKARLS
                     GGSRLVSAMDGTRLVALAEMRAGRLNPVRVFIQA"
     gene            complement(2513..2959)
                     /gene="rbfA"
                     /locus_tag="PLO65_03970"
     CDS             complement(2513..2959)
                     /gene="rbfA"
                     /locus_tag="PLO65_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRD27439.1"
                     /translation="MSRASPRHGKSRHAAPSGPSQRQLRAGELIRHALVEILREEQLA
                     DPVLADVSVTVSEVRMSPDLKHAICFVEPLGAGVTGAETQGVIKALNGASKFLRGRLG
                     RHIDMKFTPDLKFFHDDSFSTAARIDRLFDNPVVRKDLEADDAGDD"
     gene            complement(3018..3824)
                     /locus_tag="PLO65_03975"
     CDS             complement(3018..3824)
                     /locus_tag="PLO65_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD27440.1"
                     /translation="MADIDALKNYERFSFTDGPHTREVFRRGTGPAVIIMHEIPGLHP
                     LVVDYADRVAAAGMTVFLPSLFGSPGKPVSNGYALGQMFLNICIRREFNAWANGRSSQ
                     IVDWLRALARKVHAECGGPGVGALGMCFTGGFALAMMTEPSVVAPVLSQPSLPLGRKG
                     ARTIDCSAAEIRCAKQRFEDEDLSLLGLAFHGDPFVPPGRFEMLRETFGDRFEAIQLR
                     QEDAQPGTGVPGHSVLTIHLASSGPTRAAEARTIAFFRQRLGLDSAAADA"
     gene            complement(3883..4446)
                     /locus_tag="PLO65_03980"
     CDS             complement(3883..4446)
                     /locus_tag="PLO65_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27441.1"
                     /translation="MSRPLHIALVAALIASAAAPAWAAQKRMVTYDSASPTARRLTGA
                     GLTFVFTKRFMRTRILAVRATAVPVGILPRPSDDGQVNRKLDALMGEDAGRGALYSID
                     PAAQEGRVMIQAFCPGSSRGWLSIGPIAPHRDLRVHAFGDDPTTGEPRLCATMDFAFH
                     GEWRMPGPINDGVTDRVFRPDYGKPGT"
     gene            complement(4455..4979)
                     /locus_tag="PLO65_03985"
     CDS             complement(4455..4979)
                     /locus_tag="PLO65_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HRD27442.1"
                     /translation="MVRTFALLALAVVALAGCATASRFDAAGDVHSLLVAIRDNDRAE
                     FDRYVDRKALKQNLEGRLMRETSRADMDDGARAVAALLAPSLADVAVEALIRPEVFRA
                     AASYYGYTPDKPLPDRLAIGGALKYLDDGRVCATKKKNGPCLLVFTNVAGTWKLSGFE
                     GDLSDLRGKPRKTP"
     gene            complement(5002..5478)
                     /locus_tag="PLO65_03990"
     CDS             complement(5002..5478)
                     /locus_tag="PLO65_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27443.1"
                     /translation="MVAITRKHILAGVVGGAVAGPLDLLAATAQFGAPLDVICRSVAA
                     GWVGRDVAMAGGLPMALLGFFSDLGISVVAALIYCVVTPPREIARPWLNGTLFGLAMF
                     TVMRFIITPLSLAGHGKMAPTVLAESLLVHAFIFGLPVALVASLVLKPVADRPISA"
     gene            5722..6246
                     /locus_tag="PLO65_03995"
     CDS             5722..6246
                     /locus_tag="PLO65_03995"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRD27444.1"
                     /translation="MTPQEQIERYLSGQPEPKRGDLRTLHALILATAPGARLWFLDGR
                     DDNGKTVSNPNVGYGDYTIRYANGSTRDFYRVGLSANSAGISVYVMGLADKTHLSRTY
                     GQAIGKASVSGYCIKFRSLKDIDIHVLESAIRHRMELPDEGQGRRGPLNAPEPEDVTD
                     LPTRGQPHRHRERI"
     gene            6249..6725
                     /locus_tag="PLO65_04000"
     CDS             6249..6725
                     /locus_tag="PLO65_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27445.1"
                     /translation="MRKTTICLWYARDAEAAARFYAATFPDTTVGAVHRAPGDYPDGK
                     AGDVLTVEFTVMGIPCIGLNGGDAFKQSEAFSFQVSTEDQAETDRYWNAVVGNGGQES
                     QCGWCKDRWGVSWQITPRVLTEAMAKGGETAKRAFEAMMPMQKIDVAAIEAAVRGA"
     gene            complement(7038..7646)
                     /locus_tag="PLO65_04005"
     CDS             complement(7038..7646)
                     /locus_tag="PLO65_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27446.1"
                     /translation="MNMVPAGYLLKRVAVTPAWFPTTGLVDVCSVADCVNENVVDLQR
                     VWRHNGYGVANDPRELWSLAALAESDAAECTLFYYEVYEKEIESDGWTFEPDAWRPLS
                     KIDSGVEGDVVAPSSTLALDLLGFDVVVFGDFLEHSPLSCNSVAEAGRVNRHCLFDTQ
                     EDARAAIETGLFGGGCEEGVYRIFSVSKVTRGNFDIRSSAGT"
     gene            complement(7734..>9430)
                     /gene="infB"
                     /locus_tag="PLO65_04010"
     CDS             complement(7734..>9430)
                     /gene="infB"
                     /locus_tag="PLO65_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRD27447.1"
                     /translation="DIIKFLMRQGVMLKINDVIDNDTAELVATEFGHTVRRVSEADVE
                     EGFLDADDHDDHLEPRAPVVTVMGHVDHGKTSLLDALRATDVVSGEAGGITQHIGAYQ
                     VRLKGGERVTFLDTPGHAAFSAMRARGANITDIVILVVAADDGVMPQTIEAIQHAKAA
                     GVPMIVAVNKIDKPDANSTRVVNELLQHEIVVESLGGDTQMVEVSAKTGVGLDELVER
                     ILLQAEVLDLKANPDRTAEGVVIESKLDKGRGAVATVLVKRGTLKRGDIVVAGAAWGK
                     VRALLNERDEQLTEAGPSTPVEILGLDGTPDPGEPFAVVENEARARELTEYRVRVKRQ
                     RSAASGPMAGASMADMMAKLQDKRISELPLVIKGDVQGSVEAIVGALEKIGTDEVRAR
                     IILSGAGAISESDVMLAKGAGAPLLGFNVRASRQARDLADREGVEIRYYSIIYDLIDD
                     IKGVLSGMLAPIQRETFLGNAEVLQAFDISKVGKVAGCRVTEGVVRKGAKVRIVRENV
                     VVLELGTLQTLKRFKDEVPEVNAGQECGMAFAGFQDIRPGDQIECFTVEEVKRQLA"
BASE COUNT         1468 a         3095 c         3303 g         1564 t
ORIGIN      
        1 tctggcggac cttgtcgacc gtgcggccgt cgatgcggat gccggtgtcg aggatcccgc
       61 gacggaccac gtccgcctcg agttccttga acaccccggc cagcttgttg acgtcatagc
      121 cgtccgggtt ggtctccgac ttgccgaggg tcgtcatgac cttggccttg gccgcgccga
      181 tggcgtcgtg acgctcgccc ttgcgggtga tcttgtagcc ggcggcgatg tcggccccga
      241 ccagatcctt catcttcgcc ttcagggcgt cggtgtcttc ggtctcgaac tcgaacggct
      301 ccttggccgc gtgctcggcc agttcgatga tcgcgtcgat caccggctgc atgcccttgt
      361 gggcgaagga gacgccgccc aggacgacct cttccgacag ctcctggatt tccgattcga
      421 ccatcatcac ggcgtcggcg gtgccggcca cgacgaggtc catcttcgtt tccttcatct
      481 ggtcgagcgt cgggttcagc acgtactcgc catcgacgta gccgacgcgg gcggcggcga
      541 tcggacccat gaagggggcg ccggagatga ccagggcggc cgaggcgccg atcatggcga
      601 cgatgtccgg atcattctcc aggtcgtggc tcagcacggt ggcgacgacc aggacctcgt
      661 tcttgaagcc cttgacgaac agcgggcgga tcggacggtc gatcaggcgg ctggtcaggg
      721 tttccttctg cgacggggcg gtctcgcggc gcgggaagct gccggggatc ttgccggcgg
      781 cgtagaactt ttcctggtag ttgacggtca gggggaagaa gtcctggccg ggcttggggc
      841 tcttggcgaa ggtcacggcg gccagcacgg tggtgtcgcc gtaggtgacg accacggcgc
      901 cgtcggcctg gcgggccatg cggccggttt cgatggtcag cggacggccg ccccattcga
      961 tggtcttgcg cttgatgtcg aacattttga gggttttctt tctctgagtc ccgcgggcgt
     1021 attcctcacg ggggcggaca ggcgtcgttt ccgaaatcct ctccagatgt caggcccggt
     1081 gaggatttcg attgaaccct cgtcgtacgg cttgggtgga gccttggctc cgaccatccg
     1141 gttccagagc ctggaggaac gggatgggtc tcttgcagtt tgcgtccttc gacacggccc
     1201 ttcgggcctg ctcaggatga cgtgctgttg tgtacgtcat ggtgagcagc gatcctgaaa
     1261 ggatcgcgtg tcgaaccacg cgggtttgag cctagcggcg caggcccagc ttttcgatga
     1321 tcgccgtgta gcggctctcg tcggacgtct tcaggtggtc gagcagaccg cgacgctggg
     1381 agaccagctt cagcaggcca cgacggctgt ggttgtcctt cttgtgggtc ttgaagtgct
     1441 cggtcaggtt ggcgatccgt tcggacagga tcgcgacctg gacctcggcg cttccggtat
     1501 cgcctgcggt gcggccatgt tcagcgatga gcgcggcctt gcgctccagg gtgatcgaca
     1561 tcgggttaag tctccgctca ggcttgaata aagacccgga cgggattgag ccttcccgca
     1621 cgcatctcgg ccagggccac cagtcttgtc ccgtccatgg ccgaaacgag ccgcgatccg
     1681 ccggaaaggc gggccttgag cgtctcgacc tgtctgggaa ccagaacgat gggtcgtccc
     1741 tgagccagcc ggaaggcgtc ctccgcggtc acggccagcg ccgggatgtc gtccagcgcg
     1801 gtctcgaccg ggagcagaac ctccggaagc gcggccttat ggctcaaatc ttccagtttt
     1861 tccagcgtta tcgcacgatc ctcgccgaac gggccgacgc gggtgcgacg cagctggctg
     1921 acatggccgc aggcgccgag ggcctcggcc aggtcgcgga cgatcgcgcg cacataggcg
     1981 cccttgccgc attccatctc cagctcgatg tggtcggcgt ccggaaccgc ggagacgcgc
     2041 agggcgtgga ccttgatggt gcgggcccgg agttcgaagt cctcgccggc gcgggccagg
     2101 tcataggcgc gttcgccgtc gaccttgacc gccgaatagt ccggcggaac ctgctggatc
     2161 tcgccgacga aggcgggcag ggcggcctcg acctgttcgg gcgtcggccg cgcgtcggag
     2221 gtggccgtgg tggctccctc gcggtcgagg gtggccgtcg agcggcccca ttcgatggtg
     2281 aagcggtagg ccttgtcggc gtccatcatg aacggcacgg tcttggtcgc ctcgccgagc
     2341 gcgatcggca gcacgccggt ggccagcggg tcgagcgttc cggcgtggcc ggccttctgc
     2401 gccctgaaca accagcgcac cttgccgacc gcctgggtcg agccgaagtc atagggcttg
     2461 tcgaggcaga cccagccgga gatcgcctcg cccttcttgc gccgggccat gctcagtcgt
     2521 ccccggcgtc gtcggcctcg aggtccttgc ggacgaccgg gttgtcgaac agccggtcaa
     2581 tgcgggcggc ggtggagaag ctgtcgtcgt ggaagaactt caggtccggc gtgaacttca
     2641 tgtcgatgtg ccggccgagc cggccgcgca ggaacttcga ggcgccgttg agggccttga
     2701 tcacgccctg ggtctcggcc ccggtcacgc ccgcgcccag cggctcgacg aaacagatgg
     2761 cgtgcttgag atcggggctc atgcgcacct cggacaccgt gaccgagaca tcggccagga
     2821 ccggatcggc gagctgctcc tcgcgcagga tctccaccag ggcgtggcgg atcagctcgc
     2881 ccgcgcgcag ctggcgctgc gaggggccgg acggggcggc gtgacgggat ttcccgtggc
     2941 ggggggaggc gcgtgacatg gtctgtatcc tgtcgccgag aggggatcga acccggtgcg
     3001 gcggaaaggg gcggcttcta ggcgtcggcg gcggcgctgt ccagtccgag ccgctggcgg
     3061 aagaaggcga tggtccgcgc ctcggccgcc ctggtcgggc cgctgctggc caggtgaatg
     3121 gtcagcaccg agtggcccgg gacgccggtg cccggctggg cgtcctcctg ccgcagctgg
     3181 atcgcctcga agcggtcgcc gaaggtctcc ctgagcatct cgaaccggcc gggcggcacg
     3241 aaggggtcgc cgtggaaggc caggcccagc agcgacaggt cctcgtcctc gaagcgctgc
     3301 ttcgcgcagc ggatctcggc ggccgagcag tcgatggtcc gggcgccctt cctgcccagc
     3361 ggcagggacg gctggctgag caccggagcg accaccgacg gctcggtcat catcgccagg
     3421 gcgaagccgc cggtgaaaca catgcccagg gctccgacgc ccggcccgcc gcactcggcg
     3481 tggaccttgc gggccagggc gcggagccag tcgacgatct gcgaggagcg cccgttggcc
     3541 caggcgttga actcgcggcg gatgcagatg ttgaggaaca tctggcccag ggcgtagccg
     3601 ttcgacaccg gcttgcccgg tgagccgaac aggctgggca ggaacaccgt catgccggcc
     3661 gccgccaccc ggtcggcgta gtccacgacc agcgggtgca gccccgggat ctcgtgcatg
     3721 atgatcaccg ccggcccggt cccgcgccgg aacacctcgc gcgtatgcgg cccgtcggtg
     3781 aagctgaacc gctcgtagtt cttcagcgcg tcgatgtcgg ccatggcgtt cctccgttcc
     3841 cggtggaacg gtaggcgggg cgggcgtcga aagggaagag gattacgtcc ccggcttgcc
     3901 gtagtcggga cggaatacgc ggtcggtgac accgtcgttg atcgggccgg gcatgcgcca
     3961 ttcgccgtgg aaggcgaagt ccatggtcgc gcacagccgg ggctcgccgg tcgtcgggtc
     4021 gtcgccgaag gcatgcaccc gcaggtcgcg gtgcggggcg atcgggccga tcgacagcca
     4081 gccgcggctc gagcccggac agaaggcctg gatcatcacc cggccctcct gggcggcggg
     4141 gtcgatcgaa tagagggcgc cgcggccggc gtcctcgccc atcagggcgt cgagcttgcg
     4201 gttgacctgg ccgtcgtccg acggccgcgg caggatgccg accggcaccg ccgtggcccg
     4261 caccgccagg atgcgggtgc gcatgaaccg cttggtgaac acgaaggtca gcccggcccc
     4321 ggtcagccgc ctggcggtcg gcgaggccga gtcgtaggtg accatccgct tctgggcggc
     4381 ccaggccggc gcggcggcgg acgcgatgag ggccgccacc agggcgatgt ggagcgggcg
     4441 ggacatcgga agccttatgg agtcttgcgg ggtttgccgc gcaggtcgga caggtcgccc
     4501 tcgaagcccg acagcttcca ggttccggcg acattggtga acaccagcag gcaggggccg
     4561 ttcttcttct tcgtcgcgca gacccggccg tcgtccagat atttcagggc gccgccgatc
     4621 gccagccggt cgggcagcgg cttgtcgggg gtatagccgt agtagctggc cgcggcgcgg
     4681 aacacctccg gccggatcag cgcctcgacg gcgacgtcgg ccaggctcgg ggccagcagg
     4741 gcggccacgg cgcgggcgcc gtcgtccatg tcggcgcggc tggtctcgcg catcagccga
     4801 ccctcgaggt tctgtttcag ggccttgcgg tcgacgtagc ggtcgaattc ggcgcggtcg
     4861 ttgtcgcgga tggccacaag caggctgtgc acgtccccgg ccgcgtcgaa ccggctggcc
     4921 gtggcgcagc cggcgagggc gacgacagcg agcgcgagga gggcgaacgt gcggaccatg
     4981 acgggttcct tcgcgccgat cctaggccga aatggggcga tccgcgaccg gcttcagcac
     5041 caggctggcg accagagcca ccggcaggcc gaagatgaac gcgtgcacca gcaggctctc
     5101 ggccagcacc gtcggggcca tcttgccatg cccggccagg ctgagcgggg tgatgatgaa
     5161 gcgcatgacc gtgaacatgg ccaggccgaa cagggtcccg ttcagccagg gccgggcgat
     5221 ctcgcgcggc ggggtgacga cgcagtagat cagggccgcg acgaccgaga tgccgaggtc
     5281 ggagaagaac cccagcagcg ccatcggcaa cccgccggcc atggcgacgt cccgcccgac
     5341 ccagccggcc gcgaccgacc ggcagatgac atccagcggc gcgccgaact gcgccgtggc
     5401 ggccagcagg tcgagaggac cggcgacggc cccgccgaca acgccggcga ggatgtgctt
     5461 gcgggtgatc gcgaccatgg aaattccccc gatgtcatga tggcccggcg tgtccgggcc
     5521 ggccaggcgc aaggtcgggg gcagaacagg gcgccgcgag ggccgggtca aggtcggcga
     5581 cgacgagagc cgggtccgcg aatttaatgt cggccgatgt cggtttccgg caggctcgtt
     5641 cgtcgtatcc gggacgccag ggtctggcgc ccgccaaacg ccatgcccgt tcatgaccgt
     5701 cgcgacgcag agagatcgtc catgaccccg caagagcaga tcgagcgtta cctctccggt
     5761 cagcccgaac caaagcgcgg cgaccttcgg acgttgcacg cgctcatcct ggcgaccgcg
     5821 ccgggcgccc gactgtggtt cctcgacggc agggacgaca acggaaagac cgtctcgaac
     5881 cccaatgtcg gctacggcga ctacacgatc cgctacgcca acggatccac gcgggatttc
     5941 tacagggtcg gtctgagcgc caattccgcg ggcatttccg tctacgtcat gggacttgcc
     6001 gacaagacgc acctgtcccg cacctatggc caggcgatcg gcaaggcgag cgtcagcgga
     6061 tactgcatca agttcagatc cctgaaggac atcgacatcc acgtccttga gtccgccatt
     6121 cgtcaccgga tggagctccc cgatgaaggc cagggccggc ggggcccgct gaacgccccc
     6181 gaacctgaag acgttactga cctgccgacg cgcggtcagc cacaccgcca tcgtgaaagg
     6241 atctgacaat gcgcaagacc accatctgcc tctggtacgc ccgtgacgcc gaggcggccg
     6301 ccagattcta tgcggcgaca ttccccgaca ccacggtcgg cgccgtccac cgcgcgcccg
     6361 gcgactatcc cgacggcaag gcgggcgatg tgctgaccgt cgagttcacc gtcatgggca
     6421 tcccctgcat tggcctcaac ggcggcgacg cgttcaaaca gagcgaggcc ttctcgttcc
     6481 aggtttcgac cgaggaccag gccgagaccg accgctactg gaacgccgtc gtcggcaatg
     6541 gcgggcagga aagccagtgt ggctggtgca aggacagatg gggcgtcagc tggcagatca
     6601 ccccgcgcgt cctgaccgag gcgatggcca agggcggcga gaccgcgaag cgggccttcg
     6661 aggcgatgat gccgatgcag aagatcgacg tggcggcgat cgaggcggcg gtgcggggag
     6721 cgtagcgtgc gactccctct ccccttgcgg gagaaggtgg cccgcgcagc gggtcggatg
     6781 aggggttgaa tgcggggtca gcccgtcacc ccagccgacc ggcccgctcg acccctcatc
     6841 cgtcaggctt cgcctgacac cttctcccgc aaggggagaa gggcaattga gccagtcctg
     6901 cccctgatct catggcgaga ggatcgccgg tctatccagc ctgctcaagg gcgcatcacg
     6961 tcgcggcgcg atggcccgca gggccaccct tgaccaggcc ggatagaccg gcacgctggc
     7021 ctgcaagaga tcgatggcta ggttccggcg gacgatctga tgtcgaagtt gccgcgggtc
     7081 accttgctga cagaaaatat gcggtagacg ccttcttcgc acccgccgcc gaacaggcct
     7141 gtttcaatgg ccgcccgtgc gtcttcctgt gtgtcgaaca agcagtgccg gttgacccga
     7201 ccggcttccg cgacgctgtt gcaggacaac ggcgagtgct cgaggaaatc gccgaaaacc
     7261 accacgtcga agcccagaag gtccagcgcc agagtcgacg acggggcgac gacgtcacct
     7321 tcaacgccgg agtcgatttt cgagagaggc cgccaagcgt ccggctcgaa tgtccatccg
     7381 tccgactcga tctccttttc gtagacctcg tagtagaaaa gtgtgcattc ggcggcgtcc
     7441 gattcagcaa gcgctgcaag gctccagagt tcgcgagggt cgttcgcaac gccatacccg
     7501 ttgtgccgcc agaccctttg cagatcgacg acgttctcgt tcacgcagtc ggcgacactg
     7561 caaacgtcga ccagacccgt tgttggaaac caggcggggg tcacggcaac gcgcttcaga
     7621 agatagcctg cggggaccat gttcaactat aggcggcgcc cgtgttcagg gatcaaggaa
     7681 aaaggcgcgg aggtttccct ccgcgccctt cccgattccg gccgtgaagc gccttacgcc
     7741 agctggcgct tgacctcttc gacggtgaag cactcgatct ggtcgcccgg gcggatgtcc
     7801 tggaagccgg cgaaggccat gccgcattcc tggcccgcgt tgacctccgg cacttcgtcc
     7861 ttgaagcgct tgagggtctg cagggtgccc agttccagca cgaccacgtt ctcgcgcacg
     7921 atgcggacct tggcgccctt gcggaccacg ccctcggtga cccggcagcc ggcgaccttg
     7981 ccgaccttgg agatgtcgaa ggcctgcagc acctcggcgt tgccgaggaa ggtttcccgc
     8041 tggatcggcg ccagcatgcc cgagagcacg cctttgatgt cgtcgatcag gtcgtagatg
     8101 atcgaatagt agcggatctc gacgccttcg cgatcggcca ggtcacgggc ctgacggctg
     8161 gcgcggacgt tgaagccgag cagcggggcg ccggcgccct tggccagcat cacgtcgctt
     8221 tcgctgatcg cgccggcccc ggacaggatg atccgcgcgc ggacctcgtc cgtgccgatc
     8281 ttctccagcg cgccgacgat ggcctcgacg gaaccctgca cgtcgccctt gatgaccagg
     8341 ggcagttcgc tgatccgctt gtcctgcagc ttggccatca tgtcggccat cgaggcgccg
     8401 gccatcgggc cgctggcggc ggagcgctgg cgcttcaccc gcacgcggta ctcggtcagc
     8461 tcgcgggcgc gggcttcgtt ctcgaccacg gcgaacggtt cgcccggatc gggcgtgccg
     8521 tcgaggccga ggatctcgac cggagtcgag gggccggcct cggtcagctg ttcgtcccgc
     8581 tcgttgagca gggcgcgcac cttgccccag gcggcgccgg ccacgacgat gtcgccgcgc
     8641 ttgagggtgc cgcgcttgac cagcaccgtg gcgacggcgc cgcggccctt gtcgagcttg
     8701 gattcgatga ccacgccctc ggccgtgcgg tcggggttgg ccttgagatc cagcacctcg
     8761 gcctgcagca ggatgcgctc gaccagttcg tcgaggccga cgccggtctt ggccgagacc
     8821 tcgaccatct gggtgtcgcc acccaggctt tcgacgacga tctcgtgctg cagcagctcg
     8881 ttgaccacgc gggtggagtt ggcgtccggc ttgtcgatct tgttgaccgc cacgatcatc
     8941 ggcaccccgg ccgccttggc gtgctggatg gcctcgatgg tctggggcat cacgccgtcg
     9001 tccgccgcca ccaccaggat gacgatgtcg gtgatgttgg cgccgcgggc gcgcatggcg
     9061 ctgaaggccg cgtggccggg cgtgtcgagg aaggtgacgc gctcgccgcc cttcaggcgc
     9121 acctgatagg cgccgatgtg ctgggtgatg ccgccggcct cgcccgagac cacgtcggtc
     9181 gcgcgcaggg cgtcgagcag gctggtcttg ccgtgatcga cgtgacccat gacggtcacg
     9241 accggggcgc ggggctccag gtgatcgtcg tggtcgtcgg cgtcgaggaa gccctcctcc
     9301 acgtcggcct ccgacacgcg gcgcacggtg tggccgaact cggtggccac cagctcggcg
     9361 gtgtcgttgt cgatcacgtc gttgatcttc agcatcacgc cctgacgcat caggaacttg
     9421 atgatgtcga
//
LOCUS       DAORNX010000058         9393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5415_length_9393_cov_8.769865, whole genome shotgun sequence.
ACCESSION   DAORNX010000058 DAORNX010000000
VERSION     DAORNX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_9393_cov_8.769865"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /gene="rpsH"
                     /locus_tag="PLO65_04015"
     CDS             <1..223
                     /gene="rpsH"
                     /locus_tag="PLO65_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRD27448.1"
                     /translation="EFEIELKYFDNEPVIVEIARVSKPGRRVYSSIKDLKPIKNGLGI
                     SILSTPKGVMSDTAARDANVGGEVLCRVY"
     gene            225..758
                     /gene="rplF"
                     /locus_tag="PLO65_04020"
     CDS             225..758
                     /gene="rplF"
                     /locus_tag="PLO65_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRD27449.1"
                     /translation="MSRIGKKTVPVPAGVTVTLDGQTVTVKGPKGQLAWTIVEEIELR
                     QDGGELTLVPRNDTTRAKAMWGLSRTLVANMVHGVTQGFEETLELVGVGYRAALKGSD
                     LSLALGFSHDVDIPAPAGITFAVPKQTEVKIAGIDKQLVGETAAKIRRIRPPEPYKGK
                     GVRYAGETVRRKEGKKK"
     gene            761..1117
                     /gene="rplR"
                     /locus_tag="PLO65_04025"
     CDS             761..1117
                     /gene="rplR"
                     /locus_tag="PLO65_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRD27450.1"
                     /translation="MALSPRESHARRAQRTRTRLRALSNGRPRLSVFRSSKNIYAQII
                     DDENGVTLAAASSLEGGKGAKGADKDAAARVGKLVAERAIEKGVKDVVFDRGGFIYHG
                     RIKALADAAREAGLNF"
     gene            1131..1739
                     /gene="rpsE"
                     /locus_tag="PLO65_04030"
     CDS             1131..1739
                     /gene="rpsE"
                     /locus_tag="PLO65_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRD27451.1"
                     /translation="MAREPRSGGGGGERRDRRDRNAPTDDRADSDIVEKLVHINRVAA
                     TVKGGRRFSFAALMVVGDQKGRVGYGHGKAREVPEAIRKATEEAKKTMIRVPLRESRT
                     LHHDGNGRWGAGKVMVRAAPPGTGVIAGGPMRAVLETLGVQDVVGKSTGSSNPYNMVR
                     ATFEALKAQSSPRQIAAKRGKKVSDVLGRRADGASAPDAIEG"
     gene            1743..1928
                     /gene="rpmD"
                     /locus_tag="PLO65_04035"
     CDS             1743..1928
                     /gene="rpmD"
                     /locus_tag="PLO65_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRD27452.1"
                     /translation="MAEKKTVTVRQTASPLRRKGDQRATLAGLGLNKIGRTRTLEDTP
                     SIRGMIAKVAHMVEVVD"
     gene            2106..2591
                     /gene="rplO"
                     /locus_tag="PLO65_04040"
     CDS             2106..2591
                     /gene="rplO"
                     /locus_tag="PLO65_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRD27453.1"
                     /translation="MTKLNELAPREGSTRNRMRVGRGPGSGKGKTAGRGVKGQKSRTG
                     VAIAGFEGGQMPLHMRMPKRGFNNPNAKDFAEVNFWRIEQAIAAGKLDASKAIDADVL
                     LAAGVIRRKKDGVKLLGKGELSSKIEITVYHASAAAQAAVEKAGGKLTLTRPAKVEAE
                     A"
     gene            2671..4032
                     /gene="secY"
                     /locus_tag="PLO65_04045"
     CDS             2671..4032
                     /gene="secY"
                     /locus_tag="PLO65_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRD27454.1"
                     /translation="MASAAEQLAANLNFGAFQKATELHKRIMFTLIAMVVYRLGTYIP
                     IPGIDPVAFAQAFTRQSEGVLGMFNMFSGGAVQRMAIFALNVMPYISASIIVQLLGTV
                     YPPWEKLRKEGGEAGRKTLNQYTRYLTVVLALFQSAGIAFGLQNSQGVVDPNIAPLFF
                     TVSTIVTLTGGTLFLMWLGEQITSRGVGNGISLIIFAGIVAVLPRSVWQLLEQVQVGA
                     IQGWAVFMLMALAVGVILAIVFMERSQRRLLIQYPKQQRGNRMVGGESSFLPLKINTS
                     GVIPPIFASSLLLLPATVMGFVATANLPGWLQWLPPVVGQLQHGQPLFMLFYGGLIVF
                     FCFFYTSVVFNPEDTAENLRKYGGFLPGIRPGKRTAEYLDFVLTRLTVIGAAYIAAVC
                     LLPEFLISFMGSPQQTRALGGTSILIVVSVTMDTVAQIQSHLLAHQYEGLIKKSKLRG
                     GRK"
     gene            4087..4653
                     /locus_tag="PLO65_04050"
     CDS             4087..4653
                     /locus_tag="PLO65_04050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRD27455.1"
                     /translation="MNLILFGPPGCGKGTQAKRLVDERGMVQLSTGDMLRAAIASGSE
                     LGERVKGVLARGDLVSDEIVIALIEERLPEAEAAGGAIFDGFPRTVAQAEALDRMLAG
                     RGQKIDVVLRLLVDDPVLLERVTGRFEQQGRPDDNPETFKTRLAAYNAQTAPLLPYYE
                     GQGKLKAVDGMGSMDAVSAAIGAALTTV"
     gene            4847..5215
                     /gene="rpsM"
                     /locus_tag="PLO65_04055"
     CDS             4847..5215
                     /gene="rpsM"
                     /locus_tag="PLO65_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRD27456.1"
                     /translation="MARIAGVNIPTNKRVVIALQYIHGIGQKSAQDIVARVGIEESRR
                     VNQLTDQEVLHIREAIDKDFTVEGDLRRETSVNIKRLMDLACYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            5228..5617
                     /gene="rpsK"
                     /locus_tag="PLO65_04060"
     CDS             5228..5617
                     /gene="rpsK"
                     /locus_tag="PLO65_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRD27457.1"
                     /translation="MAKEPARVKKRERKNITSGVAHVNASFNNTMITITDAQGNTISW
                     SSAGAMGFKGSRKSTPYAAQMAAEDAGKKAAEHGVKTLEVNVSGPGSGRESALRALQA
                     AGMTITTIRDVTPIPHNGCRPPKRRRV"
     gene            5695..6711
                     /locus_tag="PLO65_04065"
     CDS             5695..6711
                     /locus_tag="PLO65_04065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRD27458.1"
                     /translation="MIERNWNELIRPEKPMIETGADASRKARIVAEPLERGFGVTLGN
                     ALRRVLLSSLQGAAVTAVQIDGVVHEFSSLEGVREDVVDIVLNIKQLALRMHSEGPKR
                     MTLRATGPGVVTAGQIEVPSDIEILNPDHVLCTLDDGANVRMEFTVNNGKGYVPSEQN
                     RPEDAPIGLIAVDALYSPVKRVAYRVEPTRQGQSLDYDKLVLEVETNGAVGPVDAVAY
                     AARILQDQLQIFITFEEPKAKSDGEAKPELPFNPALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAGMNLHLGMDVPNWPPENIEDLAKKF
                     EDQI"
     gene            6873..7286
                     /gene="rplQ"
                     /locus_tag="PLO65_04070"
     CDS             6873..7286
                     /gene="rplQ"
                     /locus_tag="PLO65_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRD27459.1"
                     /translation="MRHGKAHRKLGRTTAHRTAMFANMSASLIKHEQIVTTLPKAKEL
                     RPIVEKLITLGKRGDLHARRQAISQVRDIEQVGKLFAVLGPRYKARNGGYIRVLKAGF
                     RHGDNAPMAVIELVDRDPAEKGKDSGPVQDFGGEE"
     gene            complement(7384..8061)
                     /locus_tag="PLO65_04075"
     CDS             complement(7384..8061)
                     /locus_tag="PLO65_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRD27460.1"
                     /translation="MGGDLAGWNDKPIIKGYVSGDHPGNLMFAIHVLLAAVVTLGGLL
                     QLLPAIRRRAPALHRWNGRLFFVIAYVMALGGLWLTWARQTYLSLVSAVSVSLNGVLI
                     LVFAALAWRLAAGRKVEAHRRWAMRAFLVVNGVWFLRVGIMAWVLVSGGGPGMNRTLS
                     GPADILLQFGSYLIPLAVLEVYLRAQRSTRSVVKRRAAILVIAMTLVTAGGVIGAIAF
                     MWGPYMM"
     gene            8346..9347
                     /locus_tag="PLO65_04080"
     CDS             8346..9347
                     /locus_tag="PLO65_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRD27461.1"
                     /translation="MSAEGLISTPRFGGRPDDVHLYGGLFAGAVGAFCFTQIVGHRLG
                     LISDIIAIAGDATCGWSWLLVRALFRSARAPRESSPLLLVLFLVASGAVLRLAGGGAE
                     SLPRMVANLGDLVSSTLLLLAAIEPLRGLTPRMDRGERRFRMAFSAGYVALLAIAVIW
                     VDGAPAGAATAHWKMTIKSVCAACALLGMAYAIWYRHRHPLSAPRPDRRRDRPAVESG
                     LAGSVRTLMVHEALHSRQSLKVADVARRLGEPEYRITQCVTGPLGFRNFNQMANHFRV
                     EEAKQRLADSAYDHLPILTIAYDCGFGSIGPFNRAFKAETGLTPQQFRRRFSLTRDT"
BASE COUNT         1694 a         3150 c         3108 g         1441 t
ORIGIN      
        1 cgaattcgag atcgagctca agtacttcga caacgagccc gtgatcgtcg agatcgcccg
       61 cgtgtccaag cctggccgcc gggtctactc ctcgatcaag gatctcaagc cgatcaagaa
      121 cggcctgggc atctcgatcc tgtcgacgcc caagggcgtc atgtccgaca cagccgcccg
      181 cgacgccaat gtcggcggcg aagtgctctg cagggtctac tgatatgagc cgcattggaa
      241 agaagaccgt tccggtgccc gccggggtta ccgtcacgct cgacggccag acggtcacgg
      301 tgaagggccc caaggggcag ctcgcctgga ccatcgtcga agagatcgaa ctccggcagg
      361 acggcggcga gctgacgctc gtcccgcgca acgacaccac ccgcgccaag gcgatgtggg
      421 gcctgtcgcg caccctggtg gccaacatgg tccacggggt cacccagggc ttcgaggaaa
      481 ccctcgaact ggtgggcgtg ggttatcgcg cggcgctcaa gggctcggac ctctcgctgg
      541 ccctcggctt cagccacgac gtggacattc cggcgccggc gggcatcacc ttcgccgtgc
      601 ccaagcagac cgaagtcaag atcgccggca tcgacaagca gctggtcggt gaaaccgcgg
      661 ccaagatccg tcgcatccgt ccgccggagc cctacaaggg caagggcgtc cggtatgccg
      721 gcgagacggt ccgccgcaag gaaggcaaga agaagtagtc atggcgctct ctcctcgcga
      781 atcccatgcg cgccgcgccc agcgtacgcg cacccgcctc cgggcgctgt cgaatggtcg
      841 tccgcgcctg tcggtcttcc gttcgagcaa gaacatctac gcccagatca tcgatgatga
      901 gaacggcgtg accctggccg ccgcctcgtc gctggaaggc ggcaagggcg ccaagggcgc
      961 ggacaaggac gccgccgccc gggtcggcaa gctcgtcgcc gaacgggcga tcgaaaaggg
     1021 cgtcaaggac gtcgtcttcg atcgcggcgg tttcatctat cacggccgga tcaaggcgct
     1081 ggcggatgcc gcgcgtgaag ccggtctgaa cttctaaggg aacccagcag atggctcgtg
     1141 aaccccgcag cggtggtggt ggcggcgaac gccgggaccg ccgtgaccgc aacgccccga
     1201 ccgacgatcg cgccgacagc gacatcgtcg agaagctggt gcacatcaac cgcgtcgccg
     1261 cgaccgtgaa gggtggccgt cgcttcagct tcgccgccct gatggtggtc ggtgaccaga
     1321 agggccgggt cggctacgga catggcaagg cgcgcgaagt gcccgaggcc atccgcaagg
     1381 cgaccgagga agccaagaag acgatgatcc gcgtgccgct gcgcgagagc cgcaccctgc
     1441 accacgacgg caacggccgc tggggcgcgg gcaaggtgat ggtccgcgcg gcgcctccgg
     1501 gcaccggcgt gatcgccggc ggtccgatgc gtgcggtgct cgaaaccctc ggcgtccagg
     1561 acgtcgtggg caagtcgacc ggctcgtcca acccctacaa catggtccgc gccacgttcg
     1621 aggcgctcaa ggcccagtcc tcgccccgcc agatcgccgc caagcgcggc aagaaggtca
     1681 gcgatgtcct gggccgccgg gccgacggcg cctccgcgcc ggacgcgatc gagggctaga
     1741 tcatggctga gaagaaaacc gtcaccgtcc gccagacggc cagtcccctg cgccgcaagg
     1801 gcgaccagcg cgccacgctc gcgggcctcg gcctgaacaa gatcggccgc acgcgcaccc
     1861 tggaggacac cccgtcgatc cgcgggatga tcgccaaggt cgcccacatg gtcgaagtgg
     1921 tcgactagac ttttcccgcc ccctcgcggg gcgggaaggc cggcttgccg gccagggtgg
     1981 gggcgtgttt cacctcccca cccggtccct tcgggaccac cctcccgtaa aggggaggga
     2041 gacatgaaaa tcagccgagt ccggaccggg tccgggcgac agatctccag ggagaggcgc
     2101 cagaaatgac gaaactcaac gaactcgccc cgcgcgaagg ctccaccagg aaccgcatgc
     2161 gcgtcggccg cggcccgggt tcgggcaagg gcaagaccgc cggtcgcggc gtcaagggcc
     2221 agaagtcccg caccggcgtc gcgatcgccg gcttcgaagg cggccagatg ccgctgcaca
     2281 tgcgcatgcc caagcgcggc ttcaacaacc ccaacgccaa ggacttcgcg gaagtgaact
     2341 tctggcgcat cgagcaggcg atcgccgccg gcaagctcga cgccagcaag gccatcgacg
     2401 ccgacgtgct gctggcggcc ggcgtgatcc gccgcaagaa ggacggggtc aagctgctgg
     2461 gcaagggcga gctgagctcg aagatcgaga tcaccgtcta tcacgcctcc gccgccgcgc
     2521 aggcggcggt ggaaaaggcc ggcggcaagc tgaccctcac ccgcccggcc aaggtcgaag
     2581 cggaagcctg acacagacgg gcggcgccgg gtgacaacac cgggcgccgt ccctatttgt
     2641 gacgctcctt aaagagtcgc ggggacctac atggcttcgg ccgccgagca gctagccgca
     2701 aacctcaact tcggcgcctt ccagaaggcg acggagctgc acaagcgcat catgttcacc
     2761 ctgatcgcga tggtggtcta tcgcctgggg acctacatcc cgatcccggg catcgatccg
     2821 gtcgcgttcg cccaggcctt cacgcgccag agcgaaggcg tgctgggcat gttcaacatg
     2881 ttctccggcg gcgccgtcca gcggatggcc atcttcgccc tgaacgtgat gccctacatc
     2941 tcggcctcga tcatcgtgca gctgctcggc acggtctatc cgccgtggga aaagctgcgc
     3001 aaggaaggcg gggaggcggg ccgcaagacc ctcaaccagt acacccgcta cctgaccgtg
     3061 gtgctggccc tgttccagtc ggccggcatc gccttcggcc tgcagaacag ccagggcgtg
     3121 gtcgacccca acatcgcgcc gctgttcttc acggtttcga ccatcgtcac cctgacgggc
     3181 ggcaccctgt tcctgatgtg gctgggcgaa cagatcacca gccgcggcgt cggcaacggc
     3241 atcagcctga tcatcttcgc cggcatcgtc gcggtgctgc cgcgcagcgt ctggcagctt
     3301 ctcgagcagg tccaggtcgg tgcgatccag ggctgggcgg tgttcatgct catggccctg
     3361 gcagtcgggg tgatcctggc catcgtgttc atggaacgct cgcagcgccg cctgctgatc
     3421 cagtatccca agcagcagcg cggcaaccgc atggtcggcg gcgagagctc gttcctgccg
     3481 ctgaagatca acacctcggg tgtgattccg ccgatcttcg cctcgtcgct gctgctgctg
     3541 ccggccacgg tgatgggctt tgtcgcgacg gccaatctgc cgggctggct gcagtggctg
     3601 ccgccggtgg tgggccagct gcagcacggc cagccgctgt tcatgctgtt ctacggcggc
     3661 ctgatcgtct tcttctgctt cttctacacc tcggtggtgt tcaatcccga ggacacggcc
     3721 gagaacctgc gcaagtacgg cggcttcctg ccggggattc gcccgggcaa gcgcacggcc
     3781 gagtatctgg atttcgtgct gacccggctg acggtgatcg gcgcggccta catcgcggcg
     3841 gtctgtctgc tgccggagtt cctgatcagc ttcatgggca gcccgcagca gacgcgggcc
     3901 ctgggcggca cctccatcct gatcgtggtc tcggtgacca tggacacggt ggcgcagatc
     3961 cagtcgcacc tgctggccca ccagtacgag ggcctgatca agaagtcgaa gctgaggggt
     4021 ggtcggaagt aggccgtcgg ttcggggagg cgctccgacg caagatcggg gcaggggaga
     4081 ctacggatga atctgatcct gttcggcccg ccggggtgcg gcaagggcac ccaggccaag
     4141 cggcttgtcg atgaacgggg catggtccag ctctcgacgg gcgacatgct gcgggcggcc
     4201 atcgcctcgg gctcggaact cggcgagcgc gtgaagggcg tgctggcgcg gggcgacctg
     4261 gtctcggacg agatcgtcat cgccctgatc gaggagcgcc tgccggaggc cgaagccgcc
     4321 ggcggcgcca tcttcgacgg cttcccgcgc acggtggccc aggccgaggc gctcgaccgg
     4381 atgctcgccg gccgcgggca gaagatcgac gtggtcctgc gcctcctggt cgatgatccg
     4441 gtgctgctgg agcgggtcac cggccgattc gaacaacagg ggcgtccgga cgacaatccg
     4501 gaaaccttca agacccgcct ggcggcctac aacgcccaga cggctccgct gctgccctat
     4561 tacgaggggc agggaaaact gaaggccgtt gacggcatgg gttcgatgga cgcggtttcc
     4621 gcggccatcg gcgcggcgct gacgacggtc tgaaacggcg ccggataggt tgaaaatcaa
     4681 ccttttccgg cattgacgga cgcgcatcga tccctataac ggtgcccttc cgcgaaaggc
     4741 gcgactcccg cgtcggctcg aagtgcaagc tgagggccgg tgcgctagtg gcgtctcgtt
     4801 ttgcgcgtgc gtccagaggg gcgcgataag acagagagat ttaggcttgg ctcgtatcgc
     4861 aggcgtcaac attccgacga acaagcgcgt ggtgatcgcg ctgcagtaca ttcacggcat
     4921 cggccagaag tccgcgcagg acatcgtcgc cagggttggc atcgaggaat cgcgccgggt
     4981 gaaccagctg accgaccagg aagtgctgca catccgggaa gccatcgaca aggacttcac
     5041 cgtcgagggt gacctgcgtc gcgagacctc ggtcaacatc aagcgactga tggacctggc
     5101 ctgctaccgc ggcctgcgtc accgcaaggg cctgccggtc cgcggccagc gcacccacac
     5161 gaacgcccgc acccgcaagg gtccggccaa gccgatcgcc ggcaagaaga agtaagagag
     5221 agcctcgatg gccaaggaac cggctcgcgt caaaaagcgc gaacgcaaga acatcacctc
     5281 gggcgtggcg cacgtgaacg cctcgttcaa caacaccatg atcaccatca ccgacgccca
     5341 gggcaacacg atctcgtggt ccagcgccgg cgcgatgggc ttcaagggct cgcgcaagtc
     5401 gaccccctac gccgcccaga tggcggccga agacgccggc aagaaggccg ccgagcatgg
     5461 tgtgaagacc ctggaagtga acgtttccgg ccccggttcg ggtcgtgaat cggccctgcg
     5521 cgcgctgcag gccgccggca tgacgatcac caccatccgc gacgtgaccc cgattccgca
     5581 taacggctgc cggccgccca agcgtcggcg agtctgagtt ttacatagct gaccctccct
     5641 tcgccggccg tgcgtttcgc ggccggcgaa cccgcgttcc aaggacatcc gcccgtgatc
     5701 gaaagaaact ggaacgagct cattcgcccc gaaaagccga tgatcgagac cggcgccgac
     5761 gccagccgca aggcgcgcat cgtcgctgaa cctctcgagc gcggcttcgg tgtgacgctc
     5821 ggcaacgcgc tgcgtcgcgt tctgctctcc tccctgcaag gcgcggccgt gaccgccgtc
     5881 cagatcgacg gcgtggttca cgagttctcc tcgctggaag gcgtgcgcga ggacgtcgtc
     5941 gacatcgtcc tcaacatcaa gcagctggcg ctgcggatgc actccgaagg ccccaagcgc
     6001 atgaccctgc gggcgaccgg ccccggcgtt gtgacggccg gccagatcga agtcccgtcg
     6061 gacatcgaga tcctcaaccc cgatcacgtg ctctgcaccc tggacgacgg cgccaacgtg
     6121 cgcatggagt tcacggtcaa caacggcaag ggctacgtcc cgtcggaaca gaaccgtccg
     6181 gaagacgccc cgatcggcct gatcgccgtc gacgccctct attcgccggt caagcgcgtc
     6241 gcctaccgcg tcgagccgac ccgtcagggc cagtcgctcg actatgacaa gctggtgctg
     6301 gaagtcgaaa ccaacggcgc cgtgggtccg gtggacgcgg tggcctacgc cgcccgcatc
     6361 ctgcaggacc agctgcagat cttcatcacc ttcgaggagc ccaaggccaa gtccgacggc
     6421 gaggcgaagc ccgagctgcc gttcaacccg gcgctgctga agaaggtcga cgagctggaa
     6481 ctgtcggtcc gttcggccaa ctgcctgaag aacgacaaca tcgtctacat cggcgacctg
     6541 atccagaaga ccgaggcgga gatgctccgc accccgaact tcggccgcaa gtcgctgaac
     6601 gagatcaagg aagtgctcgc gggcatgaac ctgcatctcg gcatggacgt gcccaactgg
     6661 ccgccggaaa acatcgaaga cctggccaag aagttcgaag accagatcta gttcgcttat
     6721 ccgcgctatc tcccggaccg gcccccaggc cggttcgggg cccgacgaag ccgcaacagc
     6781 ggtggagggc cccggcgctt cgttctgacc caggcgggaa acaggctggg atgggtgaca
     6841 acactgcggg ctgaggccca ggagagacca caatgcgtca cggtaaggca caccgtaaac
     6901 tgggtcgtac gaccgctcac cgcacggcga tgttcgccaa catgtcggcg agcctgatca
     6961 agcacgagca gatcgtcacc accctgccca aggccaagga actgcgtccg atcgtcgaga
     7021 agctgatcac gctgggcaag cgcggcgacc tgcacgcccg tcgccaggcg atcagccagg
     7081 tgcgcgacat cgagcaggtc ggcaagctct tcgccgtgct cggcccgcgc tacaaggccc
     7141 gcaacggcgg ctacatccgc gtgctcaagg ccggcttccg ccacggcgac aacgccccga
     7201 tggcggtgat cgagctggtc gaccgcgacc cggccgaaaa gggaaaggac tccggtccgg
     7261 tccaggattt cggcggcgag gaatagtcct tcgcgaccgc gaaaccgaac atgaaaggcc
     7321 ccggagcgat ccggggcctt ttgctttgcg gggcgggatg gagcaggggg cggcggcccc
     7381 ccgtcacatc atgtaggggc cccacatgaa ggcgatggcg ccgatcacac cgccggccgt
     7441 gacgagggtc atggcgatca caaggatcgc ggcgcggcgt ttgacgacgg acctcgtact
     7501 ccgctgcgcg cggagataga cctcaagcac ggccagcggg atcaggtagg atccgaattg
     7561 aagcaggata tctgccgggc ccgacagcgt gcggttcatg cccgggcctc cgcccgacac
     7621 cagcacccag gccatgatcc ccacccgcag gaaccagacg ccgttgacga caaggaaggc
     7681 gcgcatggcc cagcgccggt gcgcctcgac cttccgcccg gcggccaacc gccacgcgag
     7741 cgccgcgaaa accaggatga gcacgccatt cagggacacg gacaccgccg agacgagcga
     7801 aagatatgtt tggcgcgccc atgtcagcca cagcccgccc agggccatca cgtaggcgat
     7861 gacgaagaac agtcggccat tccagcggtg caacgccggc gccctccggc ggatcgccgg
     7921 aaggagttgc aaaagccctc ccagcgtcac caccgccgca agcagcacgt ggatggcgaa
     7981 catcagattg cccgggtgat ctccggagac gtaacccttg atgatgggct tgtcgttcca
     8041 gccggccaga tcgccgccca tggtcttgcg gccatagtgg gcggtgatca tccagatgaa
     8101 ggcgccctgg cccgccgccg cgaccaggaa ccaggcgagg ccggcccgtc gcagaagggg
     8161 cccgcccggc gaagccgtga ccgaagggcg tggaggttct aaggtcgtca cgtcgctttg
     8221 ctcctggcgt gttgtcgatg gccagggtgt gcgggatctc ggggcggcga tctcgccgat
     8281 ttggaaatcg gctagggatt tgggagtggg cctgtgaggg gtctggtcag aggaactggc
     8341 gtttgatgag cgctgaaggc ttgatctcga caccccgttt cggcggtcgt ccggacgacg
     8401 tccatctgta tggcgggctg ttcgcgggcg cggtgggcgc gttctgcttc acccagatcg
     8461 tcggacatcg cctcgggctg atctcggaca tcatcgccat cgcgggcgac gcgacgtgcg
     8521 gctggtcttg gcttctggtc cgcgcgctct ttcgatccgc cagggccccg cgcgagtcgt
     8581 cgcccctgtt gctggtgctg ttcctggtag cgtcgggggc cgtcctgcgc ctcgctggcg
     8641 gcggcgccga gtctttgccg cgtatggtgg cgaacctggg cgatctggtc agttccacac
     8701 ttctcctgct ggcggccatt gaacccttga gggggttgac cccgcgcatg gatcggggcg
     8761 agcgacgctt tcgcatggcc ttttcggccg gctacgtggc gttgctggct attgccgtca
     8821 tctgggtcga tggcgccccc gcgggcgcgg cgacggccca ttggaagatg acgatcaagt
     8881 ctgtctgtgc ggcctgcgcc ctgctgggga tggcgtacgc gatctggtac cgccatcgcc
     8941 atccgctgtc ggcgcctcgc cccgaccggc ggcgcgacag accggccgtt gagagcggcc
     9001 tggccgggtc cgttcgcact ctcatggtgc acgaggctct ccacagccgg cagagcctga
     9061 aggtcgcgga cgtggcccgc cgcctgggcg agccggaata caggatcacg cagtgcgtca
     9121 ccggccccct gggcttccgc aacttcaacc agatggccaa tcatttccgt gtcgaggaag
     9181 ccaaacagcg cctcgcggac tccgcctacg atcaccttcc cattctcaca atcgcctacg
     9241 attgcgggtt tggctcgatt ggtccgttca atcgggcgtt caaggccgag acggggctta
     9301 ccccccagca atttcgaaga aggttctcgc tgacgcggga cacctagcac ccgcgattcg
     9361 acccacgccc ccgcgcacgg tccgtgccga agc
//
LOCUS       DAORNX010000059         9176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5586_length_9176_cov_7.436027, whole genome shotgun sequence.
ACCESSION   DAORNX010000059 DAORNX010000000
VERSION     DAORNX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5586_length_9176_cov_7.436027"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLO65_04085"
     CDS             <1..758
                     /locus_tag="PLO65_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD27462.1"
                     /translation="GGAKNHGIVLPDADLDQVVRDLSGAAFGSAGERCMALPVVVPVG
                     KKIADELRERMVAEIDTLKVGLSNDADAQYGPVVSAAHRQKIADYIQIGKDEGAELVV
                     DGRDFKLQGYEKGFFIGPSFFDGVKPGMKTYHEEIFGPVLQMVRAETFEEAVELPSRH
                     QYGNGVAIFTRSGRAAREFASRVQVGMVGINVPIPVPVAYHTFGGWKRSAFGDTNQHG
                     MEGLKFWTKVKTVTARWPEGDVADSSFVIPTMG"
     gene            complement(914..2077)
                     /gene="hemW"
                     /locus_tag="PLO65_04090"
     CDS             complement(914..2077)
                     /gene="hemW"
                     /locus_tag="PLO65_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRD27463.1"
                     /translation="MTAPGPLGVYVHWPYCARICPYCDFNVVRDRKQAEQADLAAAIV
                     ADLTAQRALTGPRGLVSVFLGGGTPSLMDPAWAAAIVAAARRLWDAEPDLEVSLEANP
                     TDAEAGRFAAFAEAGVNRLSLGLQSFDDAALTFLGRNHGSAEALRAAALAARAFPRLS
                     VDMIYALPGQTVGAWTDQLARAIDLGAEHISPYQLTIEAGTAFDRAVGRGRFRPADDE
                     AGAVLYDTTQTVLEAAGFDAYEVSNHARGEAARSRHNLVYWRGRDYVGVGPGAHGRLT
                     LHGARTATEAAAKVGDYIGNVRETGLGFREPEALSPVQAAEERLLMGLRTYEGVGFDE
                     LAALGLSAAHPAAAGLVDQGLLEADPGCLRATPAGRVVLDGVTRNLILNAGHG"
     gene            complement(2074..2673)
                     /gene="rdgB"
                     /locus_tag="PLO65_04095"
     CDS             complement(2074..2673)
                     /gene="rdgB"
                     /locus_tag="PLO65_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRD27464.1"
                     /translation="MPLILQPGTTLIAATHNPGKARELVALLDGRFEVIAAGSLGLPE
                     PDETESTFTGNALLKARHAAQLSGRIAIADDSGLSVAALDGSPGIFSARWAGPTRDFA
                     AAMGKVEERLIETGSGDRSAWFTSALAVAWPDGPAVVVEGRVDGQLVFPGRGDRGFGY
                     DPIFVPDGYSHTFGEMDPAQKDALSHRARAFDKLKAALF"
     gene            3296..3832
                     /locus_tag="PLO65_04100"
     CDS             3296..3832
                     /locus_tag="PLO65_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRD27465.1"
                     /translation="MRALGPGSVSSFLKIILDVVYYALWVLIGAAALFALVALLFSFN
                     PGLLAGWLPLSGRVAAIVERGPFLASALIAVAAYMGGVLAIVGILRRIFVTLTAGDPF
                     HPENVRRLRLVGVILGGLEIGRYLLSVVLAMMTPDRIRSIDGSLNLTTWFAVLVILVL
                     AEVFREGARLRNEAELTI"
     gene            3849..4100
                     /locus_tag="PLO65_04105"
     CDS             3849..4100
                     /locus_tag="PLO65_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD27466.1"
                     /translation="MAIRCTLDRMLQERRMSLTELADRVGVTIANLSILKTGKARALR
                     FSTLDALCRELQCQPGDIVVFEPGPGMEIEDDEDGYAGA"
     gene            complement(4136..4849)
                     /gene="rph"
                     /locus_tag="PLO65_04110"
     CDS             complement(4136..4849)
                     /gene="rph"
                     /locus_tag="PLO65_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRD27467.1"
                     /translation="MRPSERAADEMRAVTLETGVNRYAEGSCLVTFGHTKVLVTASVD
                     DGVPGWMRGKGAGWVTAEYGMLPRATHTRGRREAAQGKQSGRTQEIQRLIGRSMRAVV
                     DMKALGERQIQIDCDVIQADGGTRTAAITGAWVALRLATRYLLEEGVIKTDPILDQVA
                     AVSCGVCGDVPVLDLDYEEDSSAEADSNFVLTGNGDIVEIQATGEKRGFTRAEFETLF
                     GLAEKGVGELFALQKAAVG"
     gene            4943..6019
                     /gene="hrcA"
                     /locus_tag="PLO65_04115"
     CDS             4943..6019
                     /gene="hrcA"
                     /locus_tag="PLO65_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRD27468.1"
                     /translation="MTSILTSGPARTPSLTDLDDRARDIFRRIVESYLETGDPVGSRT
                     LSRGGVQLSPASIRNTMQDLAGLGLLDSPHSSAGRMPTHAGLRLFVDGLLEVGDVGES
                     ERREIDARLSAHGRNFEEALNEASAILSGLAGGAGMVVSPSREAGVKHMEFVALDADR
                     ALAVMVFEDGQVENRLMRLSPGLTPSALQEASNFLAARLKGRTLVQAQAEIRAELDRA
                     RTELDQTAARLVEEGLAAWGGGDAADRALIVRGRANLLNDPAAAGDLARVQKLFDDLE
                     QKEQLIGLLDGVRDAQGVRIFIGAETRLFSLSGSAVIAAPYMTGRQKVLGAIGVIGPA
                     RLNYARVIPLVDYTARVLGRMLDG"
     gene            6026..6643
                     /gene="grpE"
                     /locus_tag="PLO65_04120"
     CDS             6026..6643
                     /gene="grpE"
                     /locus_tag="PLO65_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRD27469.1"
                     /translation="MTDDTAPNGHDPEIETAEEAAADDVEALKVEVAALKDQILRFAA
                     EADNTRRRAEREMNDARAYAIQKFARDLFAVADHLSAATTHAPRDFDDQAVKNFVVGV
                     EMTQKELSSVFERNGLKKIEPEKGGKFDPHLHQAMMEAPGEGVPPGMVIKTLQAGYEL
                     FGRVVRPAMVVVAAKGAPAEAAAAPAQNPYADKPENEGGSVDTKA"
     gene            6796..7914
                     /gene="dnaN"
                     /locus_tag="PLO65_04125"
     CDS             6796..7914
                     /gene="dnaN"
                     /locus_tag="PLO65_04125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRD27470.1"
                     /translation="MKLTIERAALLKALGHVQSVVERRNTIPILSNVLLTAERERVGF
                     SATDLDMEIVDGGAAQVDVPGQITAPAHTLYEIVRKLPEGADVSLTLSGDDPRLQVAA
                     GRSKFNLPVLPAGDFPVMSSDGLSPRIPVDANDLIRLIDKTRFAISTEETRYYLNGLY
                     VHTVVENGEARLRCVATDGHRLALAEMPAPEGAAGQPGVIIPRKTINEARRLLEDAGE
                     TVDFQVSAQKVRFEFGQAALTSKVIDGSFPDYMRVIPRDNQKIMMVDNALFAQAVDRV
                     ATISAEKSRSVKLAIEPGKVTLTVRNMEAGQAVEEVEVDYDGESFEIGFNARYLLDVT
                     GQIGGETAEFRFADPASPTLVLDPTDAGVKYVLMPLRV"
     gene            complement(7921..8688)
                     /locus_tag="PLO65_04130"
     CDS             complement(7921..8688)
                     /locus_tag="PLO65_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27471.1"
                     /translation="MTDEHRSATLAKVPAVTLGFWIIKILATTLGETGGDTLSMSYSL
                     GYLVSTFIFVVPLILLVIAQVRAKRFHPFLYWATIIASTTAGTTMADFATRSMGIGYP
                     GGSALLLACVLLSLLAWRLTTGTIAVESVASPRSELFYWLTITFSQTLGTALGDWAAD
                     DGGLGYLGGAALFGGVLLALVALWRFTSVSKVMLFWAAFILTRPLGATVGDFLDKPLA
                     QGGLDFSRPVASAVLAVAILALILVLPQRAGAHPARG"
     gene            8769..9080
                     /locus_tag="PLO65_04135"
     CDS             8769..9080
                     /locus_tag="PLO65_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27472.1"
                     /translation="MSLRLPQTLSSSASSVEVFDAGLQVELMAEKAASLGRAGRRVET
                     TLAGLREAAVDDRAAALKAAADAVWSLFVQREICGQRDQRPVIDVYGIPKEVLARLGA
                     R"
BASE COUNT         1410 a         3241 c         3161 g         1364 t
ORIGIN      
        1 tgggcggggc caagaaccac ggcatcgtcc tgcccgacgc cgacctcgac caggtggtca
       61 gggacctgtc gggcgcggcc ttcggctcgg ccggcgagcg ctgcatggcc ctgcccgtcg
      121 tggttccggt cggcaagaag atcgccgacg aactgcgcga gcggatggtc gccgagatcg
      181 acacgctcaa ggtcggcctg tccaacgacg ccgacgccca gtacggcccg gtggtctcgg
      241 ccgcccaccg gcagaagatc gccgactaca tccagatcgg caaggacgag ggggccgagc
      301 tggtcgtcga cggccgcgac ttcaagctgc agggctacga gaagggcttc ttcatcggcc
      361 cgagcttctt cgacggggtg aagcccggca tgaagaccta tcacgaggag atcttcggcc
      421 cggtgctgca gatggtccgc gccgagacct tcgaggaggc cgtcgagctg ccgagcaggc
      481 accagtacgg caacggcgtg gccatcttca cccgcagcgg ccgcgccgcc cgcgagttcg
      541 ccagccgggt ccaggtcggc atggtcggca tcaatgtgcc gatcccggtg ccggtcgcct
      601 accacacctt cggcggctgg aagcgcagcg ccttcggcga caccaaccag cacggcatgg
      661 aagggctgaa gttctggacc aaggtcaaga ccgtcaccgc ccgctggccc gagggtgatg
      721 tggcggattc gagcttcgtc atcccgacga tggggtagcg cccctgccac cctgcactgt
      781 catcccggac ggcccccggc gtaggccggg gggcgatccg ggacccagat tgaatctcgc
      841 ccacagtgtg tgaagccgaa tctgggtccc ggctcgccgc tgcgctgcgg ccgggatgac
      901 agtggtaggt gggctacccg tggcctgcgt tgaggatgag gttgcgcgtc accccgtcca
      961 gcacgacccg gccggccggc gtagcgcgca gacaaccggg gtcagcctcc agcaaaccct
     1021 ggtctaccag ccccgccgcg gccggatgcg ccgccgacag ccccagcgcc gccagctcgt
     1081 caaacccgac cccctcatac gtccgcaacc ccatcagcag ccgctcctcc gccgcctgga
     1141 ccggcgacag cgcctccggt tcgcggaagc ccaggcccgt ctcccgcacg ttgccgatat
     1201 agtcgccgac cttcgcggcc gcctcggtcg ccgtccgcgc cccgtgcagc gtcagccggc
     1261 catgggcgcc ggggccgacg ccgacatagt cccggccgcg ccagtagacc aggttgtgcc
     1321 gcgaccgggc ggcctcgccc cgggcgtggt tgctgacctc ataggcgtcg aacccggccg
     1381 cctcgagcac ggtctgggtc gtgtcgtaga gcaccgcccc ggcctcgtcg tcggcgggcc
     1441 tgaaccgccc gcgcccgacg gcccggtcga aggccgtgcc cgcctcgatg gtcagctggt
     1501 agggcgagat gtgctccgcg ccgaggtcga tggcccgggc cagctggtcg gtccaggcgc
     1561 ccaccgtctg accgggcagg gcgtagatca tgtccaccga caggcgtgga aaggcccgcg
     1621 ccgccaacgc cgccgcccgc agtgcttcgg cgctgccgtg gttgcggccg aggaaggtca
     1681 gggccgcgtc gtcgaatgac tgcaggccca gcgacaggcg attgaccccc gcctcggcga
     1741 aggccgcgaa ccggccggcc tccgcgtcgg tcggattggc ctccaggctg acctcgagat
     1801 cgggttcggc gtcccacagg cgccgggccg cggcgacgat cgcggccgcc caggccggat
     1861 ccatcagcga gggcgtgccg ccgccgagga acaccgagac aagcccccgg ggacccgtca
     1921 atgcgcgctg ggccgtcagg tcggcgacga tggccgcggc cagatcggcc tgctcggcct
     1981 gtttgcggtc gcgcacgacg ttgaagtcgc agtaggggca gatccgcgcg cagtagggcc
     2041 aatggacata gacccctagg ggcccgggcg cggtcaaaac agggcggcct tgagcttgtc
     2101 gaacgcccgg gcgcggtggc tgagcgcgtc cttctgcgcc gggtccattt cgccgaaggt
     2161 gtggctgtaa ccgtcgggca cgaagatcgg gtcgtagccg aagccgcgat caccccgacc
     2221 gggaaacacc agctgtccgt cgacccggcc ctcgaccacc acggccggac cgtcgggcca
     2281 ggccacggcc agggcgctgg tgaaccaggc cgagcggtcg ccggagccgg tctcgatcag
     2341 ccgctcctcg accttgccca tcgcggcggc gaagtccctt gtcgggccgg cccagcgggc
     2401 ggagaagatg cccggcgagc cgtccagcgc cgccaccgac aggcccgagt cgtcggcgat
     2461 ggcgatgcgg ccgctcagct gggccgcgtg gcgcgccttg agcagggcgt tgccggtgaa
     2521 ggtcgactcg gtctcgtcgg gctcgggcag gccgaggctg ccggcggcga tcacctcgaa
     2581 ccggccatcg agcagggcga ccagctcgcg ggccttgccc ggattgtggg tcgcggcgat
     2641 cagggtggtt ccgggctgga ggatcagcgg catgggaatg tcctttcgcg ccggccttcc
     2701 tcgccccgaa ccgccgccgg cgcaagaccg ccccggttta tgctgcaccg cattaccgcc
     2761 gcgtaatgat gttacgcaag tttaatatcg tttatcgata attttcttga tcgaagaacg
     2821 gcaaatccct agtttgggga tgcggacggg acgcgagttc ggtccgctga atgtggaggg
     2881 gtccacattc atattgcact gcaacatcaa ggagggactc atgtccgcgc aaaaaatgac
     2941 gaacctggtc gacaagatcg cgatgaccct ggtcaacgcc gctctgctgg ccgccctgcc
     3001 catggcggcg gttctgttcg tcacccactc gatctgatcg gcgccctttg atgagggccc
     3061 cgatcagggc atcctcgccg gccgctccgc ggctcgcctc tcccaaccga tgcgctccgc
     3121 atccgttcgg cgaggcgagc ccgggccaga agcggccggc gggatccggc ccggaccatg
     3181 acagccgcgg gtggacaccg atcaccgcga ccgtcacggt ctgacagcaa gaccacgccg
     3241 gcccgggatc gggccggcgt gggggaccgg aatcgatagt acgggggact gcgccatgcg
     3301 cgccttgggg ccaggctcgg tttcgagctt cctgaagatc attctcgacg tggtctacta
     3361 tgcgctgtgg gtgctgatcg gcgccgcggc gctgttcgcg ctggtggccc tgctgttcag
     3421 cttcaacccc ggcctgctgg ccggctggct gccgctgagc ggccgggtcg ccgcgatcgt
     3481 ggagcgcggc cccttcctcg cctccgccct gatcgccgtc gccgcctaca tgggcggggt
     3541 gttggccatc gtcggcatcc tgcggcgcat tttcgtgacc ctgaccgccg gcgacccgtt
     3601 tcatcccgag aacgtgcgcc ggctgcggct ggtcggggtg atcctcggcg ggctcgagat
     3661 cgggcgctac ctcctctcgg tcgtgctggc gatgatgacg ccggaccgca tcaggagcat
     3721 cgacggctcg ctgaacctga ccacctggtt cgccgtgctg gtgattctcg tcctggccga
     3781 ggtgttccgc gaaggcgccc gcctgcgcaa cgaagccgaa ctgacgatct agggggcgca
     3841 ggcgtaccgt ggccattcgc tgcacactcg accgcatgct gcaggagcga cgcatgtcgc
     3901 tgaccgagct cgccgaccgc gtcggcgtga ccatcgccaa cctgtcgatc ctcaagaccg
     3961 gcaaggcccg cgccctgcgc ttctcgaccc tcgacgccct ctgccgcgag ctgcagtgcc
     4021 agcccggcga catcgtcgtc ttcgaacctg gccccggaat ggagatcgag gacgacgagg
     4081 atggctacgc cggggcgtga ggggacgtgt acctttggta cgtgtccccg atcgcctacc
     4141 ccaccgccgc cttctgcagc gcgaacagct cgccgacgcc cttctcggcc aggccgaaca
     4201 gcgtctcgaa ctcggcccgg gtgaagccgc gcttctcgcc ggtggcctgg atctcgacga
     4261 tgtcgccatt gccggtcagc acgaagttgg agtcggcctc ggccgaggag tcctcctcgt
     4321 agtcgagatc gagcaccggg acgtcgccgc agaccccgca ggacacggcc gcgacctggt
     4381 cgaggatcgg gtcggtcttg atcacccctt cctccagcag gtagcgggtg gccagtcgca
     4441 gggcgaccca ggcgccggtg atggcggcgg tgcgggtgcc gccgtcggcc tggatcacgt
     4501 cgcagtcgat ctggatctgc cgctcgccca gcgccttcat gtcgaccacc gcgcgcatcg
     4561 agcggccgat cagtcgctgg atctcctggg tgcggcccga ctgtttgccc tgggcggcct
     4621 cgcggcggcc gcgggtgtgg gtggcccggg gcagcatgcc gtactcggcc gtcacccagc
     4681 cggcgccctt gccgcgcatc cagcccggca ccccgtcgtc gacgctggcc gtgaccagca
     4741 ccttcgtatg gccgaaagtc accaggcagg acccttcagc gtagcggttg acgcccgtct
     4801 cgagggtcac ggcgcgcatc tcgtcggcgg cgcgttcgga aggacgcatg gctaattccc
     4861 caggaacggc gcacagcttg cgcgcgggca ggcgccgctt atcctatcgc ccgacgggtc
     4921 gtccaccggc ctgagccaga acatgacatc gatccttacc agcggccccg cccgcacccc
     4981 gagcctgacc gatctggatg accgcgcgcg ggacatcttc cggcgcatcg tcgagagcta
     5041 cctcgagacc ggcgacccgg tcggctcgcg caccctctcc cggggtggcg tgcagctgtc
     5101 gccggcctcc atccgcaaca ccatgcagga cctggccggg ctgggcctgc tcgattcccc
     5161 gcactcgagc gccggccgca tgccgaccca tgcgggcctg cgcctgttcg tcgacggcct
     5221 gctcgaggtc ggtgacgtcg gcgagagcga gcggcgcgag atcgatgcgc gcctttcggc
     5281 ccacggtcgc aatttcgagg aagcgctcaa cgaggccagc gcgatcctgt cgggcctggc
     5341 gggcggggcc ggcatggtcg tcagcccctc gcgcgaggcg ggcgtcaagc acatggagtt
     5401 cgtcgccctc gacgccgacc gcgccctggc ggtgatggtc ttcgaggacg gccaggtcga
     5461 gaatcgcctg atgcgcctgt cgcccggcct gaccccctcg gcgctgcagg aggcgtcgaa
     5521 cttcctcgcc gcccggctca agggccgcac cctggtccag gcccaggccg agatccgcgc
     5581 cgagctcgat cgcgcccgga ccgagctgga ccagacggcc gcccggctgg tcgaggaggg
     5641 cctggccgcc tggggcggcg gcgacgcggc cgaccgggcc ctgatcgtgc gcggccgggc
     5701 caatctgctc aacgacccgg cggcggccgg cgacctggcc cgggtgcaga agctgttcga
     5761 cgacctggag cagaaggaac agctgatcgg tctgctcgac ggcgtgcgcg acgcccaggg
     5821 cgtgcgcatc ttcatcggcg ccgaaacgcg tcttttctcg ctttcgggtt ccgccgtgat
     5881 cgcggcgccc tatatgacgg gccgacagaa ggtgctgggc gcgatcggcg tgatcgggcc
     5941 ggcccgcctc aactacgccc gggtgatccc gctggtggac tacaccgcgc gggtcctggg
     6001 ccgcatgctg gacggataga cgagtatgac tgacgacacc gcgcccaacg gccacgaccc
     6061 cgagatcgag acggccgagg aagccgccgc cgacgacgtg gaggccctca aggtcgaggt
     6121 cgccgccctc aaggaccaga tcctgcgctt cgccgccgag gccgacaaca cgcgccgtcg
     6181 ggccgagcgc gagatgaacg acgcccgcgc ctacgccatc cagaagttcg cccgcgatct
     6241 gttcgccgtg gccgaccatc tgtcggccgc caccacccac gcgccgcgcg acttcgacga
     6301 ccaggcggtg aagaatttcg tggtcggggt cgagatgacc cagaaggagc tcagctccgt
     6361 cttcgagcgc aacggcctga agaagatcga gccggagaag ggcggcaagt tcgacccgca
     6421 cctgcaccag gcgatgatgg aagcgcccgg agagggcgtg ccgccgggga tggtgatcaa
     6481 gacgctgcag gccggctacg agctgttcgg ccgggttgtg cgcccggcga tggtcgtggt
     6541 ggccgccaag ggcgctccgg cggaggccgc cgcggccccg gcccagaacc cctacgccga
     6601 caagcccgag aacgagggcg gctcggtgga caccaaggcc tgacctgaac atcctcccct
     6661 gccttgcagg ggaggatccg tcgtccgacc cccttccttg gcgcggcatt tccgctaatg
     6721 tccatagtcc cctgacaggc gaagcggcgg gggcctcggc gattcgcctg cgaccttccg
     6781 gacttcggac gagacatgaa gcttacgatc gagcgggcgg cgctgctgaa ggctcttggc
     6841 cacgtgcaga gcgttgtcga gcggcgcaac accatcccga tcctgtcgaa cgtgctgctg
     6901 accgccgagc gcgagcgggt cggcttctcc gcgaccgacc tggacatgga gatcgtcgac
     6961 ggcggcgcgg cccaggtcga cgtgcccggc cagatcaccg cgccggccca caccctgtac
     7021 gagatcgtgc gcaaactgcc ggaaggcgcg gacgtctccc tgaccctgtc gggcgatgat
     7081 ccgcgactgc aggtcgcggc cggccgctcg aagttcaacc tgccggtgct gccggccggc
     7141 gacttcccgg tgatgagctc ggacggcctg tcgccgcgca tcccggtcga cgccaatgac
     7201 ctgatccggc tgatcgacaa gacccgtttc gccatctcca ccgaggagac gcgctactac
     7261 ctgaacggcc tgtacgtgca cacggtggtc gagaacggcg aggcgcgcct gcgctgcgtg
     7321 gccaccgatg gccaccgcct ggccctggcc gagatgccgg cccccgaggg cgcggccggc
     7381 cagccgggcg tgatcatccc gcgcaagacc atcaacgagg cccgtcgcct gctggaggac
     7441 gccggcgaga cggtcgactt ccaggtcagc gcccagaagg tccgcttcga gttcggccag
     7501 gccgccctga cctccaaggt catcgacggc tcgttccccg actacatgcg cgtgatcccg
     7561 cgcgacaacc agaagatcat gatggtcgac aacgccctgt tcgcccaggc ggtggaccgg
     7621 gtggcgacga tctcggccga gaagagccgc tcggtgaagc tggccatcga gcccggcaag
     7681 gtcaccctga ccgtgcgcaa catggaagcc ggccaggcgg tggaagaggt cgaggtcgac
     7741 tacgacggcg agtcgttcga gatcggcttc aacgcccgct acctgctcga cgtcaccggc
     7801 cagatcggcg gcgagacggc cgagttccgg ttcgcggatc ccgcgtcgcc gacgctggtg
     7861 ctcgacccga ccgacgccgg ggtgaagtac gtgctgatgc cgctgcgggt gtaggggggg
     7921 tcaacccctt gccgggtgcg cgccggcccg ctgcggcagg accaggatca gcgccaggat
     7981 cgccaccgcc agcaccgccg aggcgaccgg gcggctgaag tcgaggccac cctgggccag
     8041 tggcttgtcg aggaagtcgc cgaccgtcgc gcccagtggg cgggtgagga tgaaggcggc
     8101 ccagaacaac attaccttgc tgacggacgt gaaccgccac agcgccacga gcgccagcag
     8161 caccccgccg aacagggccg cgccgccgag atagccgagc ccgccgtcat ccgccgccca
     8221 gtcgccgagc gccgtgccga gcgtctgcga gaaggtgatc gtcagccagt agaacagttc
     8281 cgaccggggg ctggcgacag actcgaccgc gatcgtgccg gtggtcagcc gccaggccag
     8341 caacgacagc agcacgcagg ccagcagcag ggcagacccg cccggatagc cgatgcccat
     8401 cgaccgggtc gcgaagtcgg ccatggtcgt gcccgccgtg gtcgaggcga tgatcgtcgc
     8461 ccagtagagg aatgggtgga acctcttcgc ccgcacctgg gcgattacca gcaggatcaa
     8521 cggcacgacg aagatgaagg tgctgaccag gtagcccagg ctgtagctca tcgacagggt
     8581 gtcgccgccc gtctcgccca gggtggtggc gagaatcttg atgatccaga agcccagcgt
     8641 aaccgccggg accttggcca gagtcgcgct gcggtgttcg tcggtcatga tgaattcgct
     8701 ctggtcgccg cacctcgggg cggcctgcgc ctcttcatcg gcagcctacg tggcgctagt
     8761 gtggccgcgt gtccctgcgc ctgccccaga ccctgtcatc ctccgcctcg tcggtcgagg
     8821 tgttcgacgc cggtctgcag gtcgagctta tggccgagaa agccgcctct ctcgggcggg
     8881 ccggacggcg ggtcgagacg acgctggcgg gcctgcgcga ggccgcagtg gatgaccgcg
     8941 ccgccgccct gaaggccgcc gccgacgcgg tctggagcct gttcgtccag cgcgagatct
     9001 gcggccagcg ggaccagcgg ccggtcatcg acgtctacgg gattccgaaa gaggtgctgg
     9061 cccggctggg cgcgcggtag gctccgggtt tcgtctgcaa tccgatgaca cctgcccttt
     9121 gaccttgatg gaatgatgcg ctccggtcgg tcgcccactc atcttcctcc cccact
//
LOCUS       DAORNX010000060         9086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5660_length_9086_cov_6.535489, whole genome shotgun sequence.
ACCESSION   DAORNX010000060 DAORNX010000000
VERSION     DAORNX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_9086_cov_6.535489"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..311
                     /locus_tag="PLO65_04140"
     tRNA            236..311
                     /locus_tag="PLO65_04140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:269..271,aa:Thr,seq:tgt)
     gene            332..778
                     /locus_tag="PLO65_04145"
     CDS             332..778
                     /locus_tag="PLO65_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27473.1"
                     /translation="MTPPRTSDIQIQACRLFAEAIDREADGEFLAAEALYTVAAELGY
                     SLAMNNLAILIEDVITPARPAAAVYWLKRAVAAGYDEAAWNLAMRQRLAGRRRWYVHW
                     LRIARDLGHAEAADVLPTAESPGGRWWVIDGVSPPPFIRGPRLVQP"
     gene            786..1679
                     /locus_tag="PLO65_04150"
     CDS             786..1679
                     /locus_tag="PLO65_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD27474.1"
                     /translation="MADRLAAETLKGVERARKAVDFMLSRRDHALPLLAQTRNLTVEG
                     AAGPLAARLYIPREAGDSGPMLVFFHGGGFVLGDLETHDAFCVRLADTAKVRVLACRY
                     RLAPEAPFPAQLDDALAAFRAIRGRAAEFGADSDALALGGDSAGGYLAVAATARLNAE
                     SPGAVKAQVLVYPLLTLDDDVWASSLLQDSRIVGRLAVQFIRAQLATTSAPSLLGRLQ
                     GRTPPTLLVAGGPLDPCRPDALTYARQLAESGSEFQERYFTALPHGFINLTHVSAPAR
                     EAVADIGVMAGALLRRVCAGT"
     gene            1829..2302
                     /locus_tag="PLO65_04155"
     CDS             1829..2302
                     /locus_tag="PLO65_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRD27475.1"
                     /translation="MTDARAPIRIYVDADACPVKDEVYKVAGRYGLKTFVVSNSFMMV
                     PVSALVERVVVDAGPDIADDWIAERAAPGDVVVTNDIPLADRVLKAGATAIKPNGQPF
                     TADSIGGALAQRAIMEHIRSTGEITGGPRPFDRNDRSRFLQALDEAIQREKRRRR"
     gene            2299..2724
                     /gene="arfB"
                     /locus_tag="PLO65_04160"
     CDS             2299..2724
                     /gene="arfB"
                     /locus_tag="PLO65_04160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRD27476.1"
                     /translation="MIEITPAIALDESELEFAYIRAGGPGGQNVNKVATAVQMRFDAR
                     RSPSLPNDVSTRLQKLAGSRLTLDGVIVIAASRFRTQERNRADAIDRLVELIRKAAEP
                     PPPPRKKTRPSKAAKARRVDAKTRRGGIKAMRSRPGGDD"
     gene            complement(2817..3023)
                     /locus_tag="PLO65_04165"
     CDS             complement(2817..3023)
                     /locus_tag="PLO65_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD27477.1"
                     /translation="MADPRLANGLKAARTRLGLTQADLADRVGVTRKTINTVENGVFV
                     PSTVLALRLAKALGEPVEALFWLE"
     gene            complement(3016..3489)
                     /locus_tag="PLO65_04170"
     CDS             complement(3016..3489)
                     /locus_tag="PLO65_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27478.1"
                     /translation="MAGKGKPEPSLSERVERAQRRRTRLIWVQGLFFLVWQGTFLNGL
                     WTLEAPLRTVDQVKLSGFVVWGAALLLLLSTGGGWFRNRALRAILEDESTRAHRRTAL
                     VTGFWVVMILALGLYVLSLFVPMTALEAVHALLTAGVVTPAIVFVALERRAERHG"
     gene            3582..4199
                     /locus_tag="PLO65_04175"
     CDS             3582..4199
                     /locus_tag="PLO65_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRD27479.1"
                     /translation="MLRGAVASAENRASVRPHQSGELVMRAEVKVLVLVGAAALLTAC
                     SKPAETPAATEAVPADSAATPAATPAPAAKAESEESDEHAEKVLASLPAPYNTADLEN
                     GQRKFALCRSCHTIVEGGPNMTGPNLYGVFGRTAGTAPKFNYSDAVKAAGFKWDAAHL
                     DKWLADPRADLPGNRMTFVGLKDPKDRADVIAYLMVETARKSDDD"
     gene            4209..4553
                     /locus_tag="PLO65_04180"
     CDS             4209..4553
                     /locus_tag="PLO65_04180"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD27480.1"
                     /translation="MGDTEALLTRLYEAFNRKDIEAVISALHPDVSWPNLFGEGRLQG
                     HAAMRRMWREQFSKIDPEATPIRFLRQEDGSLTVLISYVVRTLDGKVFTEEIATNTYR
                     FSDGKVIGMEWA"
     gene            4636..4980
                     /locus_tag="PLO65_04185"
     CDS             4636..4980
                     /locus_tag="PLO65_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD27481.1"
                     /translation="MDDRQDTIDRLWDALNRQDIDAAAALLHPDVDWQDLMNGGRRTG
                     VADVRLYWADLFRIMRPESTSLTFTPTGDGRVAVRTHHSIRDPSGRFWTEETITHLFG
                     FTDGLLSSMDVA"
     gene            complement(4987..7341)
                     /locus_tag="PLO65_04190"
     CDS             complement(4987..7341)
                     /locus_tag="PLO65_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRD27482.1"
                     /translation="MDDAGFAVRGKDIALLRRLEERVLWLASWTIHNANHLRDSRDGL
                     KVGGHQASCASMTTLMTALYFSALRPQDRVAVKPHASPVFHAIQHLYGRQTIDQLQRF
                     RALGGAQSYPSRTKDIDDVDFSTGSVGLGVAMTAFASLTQDYLAARGAVKPDAMGRMI
                     SLLGDAELDEGNIYEALIEACKHDVRNTWWIVDYNRQSLDATTSDRMFTRYGEIFEAA
                     GWKVETLKWSRRQQQAFARPGGAALQAWIESAPNDLYSALTYQGGAAWRERLMVDLAG
                     DKAALKLIGGYDDTELALVMTELGGHCLETILEAFAKAAEDDAPRFFIAYTVKGWRLP
                     FAGHKDNHSGLMTPTQIAELRARLGVREGEEWDVFSGLADNELKAMKRLVETAPFAAR
                     KDGHRDAPNVATPTAETLLKAVEGGGEQSTQAAFGKVMFEIAKGVDEFAGRVVTTSPD
                     VTVSTNLGGFVNRRGVFHRRAHEDVFKNRRIPSAQVWAMGETGQHIELGIAENNLFIA
                     LAALGLSGPLFGERLFPVGTLYDPFIARGLDALNYACYQDARFLLVATPSGLTLAPEG
                     GAHQSIGTPLIGMAQPGLDSYEPAFADETAVLMAHAFARIQASEGGSTYLRLSTRVID
                     QPTRADDAWRAGCVAGAYWMKRPAPDARLALVYSGAIAPEALAAFEQLLEDEPGAGLL
                     AVTSPDVLHADWTAAGRARWTTGAQRTATIETLLADLDPAAGLVTVIDGSPAALSWIG
                     SVRGQRVRALGLETFGQSGDLPDLYAKYRLDPGAILDACADLMV"
     gene            complement(7354..>9086)
                     /locus_tag="PLO65_04195"
     CDS             complement(7354..>9086)
                     /locus_tag="PLO65_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD27483.1"
                     /translation="AVAFSAFLALSLSPMLASRLLRPASGNGWLASRVDLVVERVRDS
                     YRHSLEMLLASRIAGGGALALVLALAALAGTLFMVLPQELVPAEDRGRVDVSISGPEG
                     AGFDYTLKAANQVERQVRRYYEQGEAERYVVVIPRFGATQFNTGNAVLVLKPWGERTK
                     TSDQVADELNKSLGAITGVRAVASVRGAFQRGGGSGGGSNVDLVGLGNDYDEIARWLG
                     PIRDAMMRNPGFSRPRLDYEPTAPRLSVEIDREKAASLGVTPQAIGRSLETMFGSRRV
                     TTYTKGGQEYDVIVQTDLEKRRTLADLNTLYVRAGSGQLVPLSAVVTTQLRGDTPDRN
                     RTDRQRSVRLTSQLTPGFTVAEAVKFLQAEAAKRPTAGVSYTWDGQAKDYLEASGAVG
                     LAFGMALLLVFLVLAAQFESWIHPAVIMLTVPLAALGGLFGLLLTGQTINTYSQIGLI
                     ILIGVAAKNGILIVEFANQLRDQGRSIHDAVIEAAVLRLRPIVMTSITAAFGSLPLIL
                     WSGAGAASRQTIGVTIFFGAIFATLLTVFVVPVFYNMLARFTKSPEWTARQIEAYEQG
                     ETAGQASPAE"
BASE COUNT         1447 a         3176 c         3132 g         1331 t
ORIGIN      
        1 ccccggcgcg gccgcccgcc gcggccgtcg ccgaggccgc ctgagtccgg cccgaaacga
       61 agaacccccg gaccccgcag tgcggaatcc gggggcccag tgaggcttcg caaaactgtt
      121 atcgtcggga atcagccgga tcgtcaccgc gtcgaaagcg cgcagcccgc gcggccgcgc
      181 gggtcgcggc gctggcgtcg ggcagcagcc tgcggcataa ggcccagcca ttggggccgg
      241 cttagctcag ttggtagagc agcggttttg taaaccgaag gtcgcgggtt cgaatcctgc
      301 agccggcacc atgccaggac cggacgctcc cgtgaccccg ccgcgaactt ccgacataca
      361 gatccaggcc tgccggttgt tcgccgaggc gatcgatcgg gaagccgacg gagagtttct
      421 ggcggccgag gccctctaca ccgtcgccgc cgaactgggc tattcgctgg ccatgaacaa
      481 cctggcgatc ctgatcgagg acgtgatcac gcccgcccgc cccgccgccg ccgtctactg
      541 gctcaagcgg gcggtggcgg ccggctatga cgaggcggcc tggaacctgg ccatgcgcca
      601 gcgactcgcc gggcggcggc gctggtacgt ccactggctg aggatcgcgc gggatctcgg
      661 tcacgccgag gcggctgacg tcctgccgac cgcggaatcc cccggcgggc gctggtgggt
      721 catcgacggc gtcagcccgc cgcctttcat caggggaccg cgccttgtcc agccctgacc
      781 ggtcgatggc cgaccgcctc gccgccgaaa ccctcaaggg cgtcgaacgg gcccgcaagg
      841 ccgtcgactt catgctgtcg cgacgcgacc acgccctgcc gctgctggcg cagacccgca
      901 acctgaccgt cgagggcgct gccggtccgc tggccgcgcg actgtacatc ccccgggagg
      961 ccggggattc cgggccgatg ctggtgttct ttcatggcgg cggcttcgtg ctgggcgacc
     1021 tggaaaccca tgacgccttc tgcgtccgcc tggcggacac ggcgaaggtg cgggtgctgg
     1081 cctgccgcta tcgcctggcc ccggaggcgc ccttcccggc ccagctggac gacgccctgg
     1141 ccgccttccg cgcgattcgt ggccgggccg ccgagttcgg cgccgattcc gacgccctgg
     1201 ccctgggcgg ggattccgcc ggcggctacc tcgccgtggc cgccaccgcc cggctcaacg
     1261 cggagagccc gggcgcggtg aaggcccagg tgctggtcta tccgctgttg accctggacg
     1321 acgacgtctg ggcctcgagc ctgctgcagg actcccgcat cgtcggccgc ctggcggtgc
     1381 agttcatccg cgctcagctg gcgaccacct cggcgccgtc cctgctgggg cggctccagg
     1441 gcaggacgcc gccgacgctt ctggtggccg gaggtcccct cgatccctgc cggcccgacg
     1501 ccctgaccta tgcgcgacag ctggcggaga gcgggagcga gttccaggag cgctacttca
     1561 ccgccctgcc gcacggcttc atcaacctga cccatgtctc cgcgccggcg cgcgaggccg
     1621 tcgcggacat cggcgtgatg gcgggagcct tgctgcggcg ggtttgcgcg ggaacctagg
     1681 cccccgcggt catcgtccag atgaacgcgt cgatatcggc caggaaacgc ttgaggaaca
     1741 gtggcatggc gatggactcc ccgatgcact ttgacgtctc gttaacgatt gttggctgat
     1801 tcgggttcct ctggctaaga cgctggcgat gaccgacgcc cgcgccccga tccgcatcta
     1861 tgtcgacgcc gacgcctgcc cggtgaagga cgaggtctac aaggtcgccg ggcgctacgg
     1921 gctgaagacc ttcgtggtct ccaacagctt catgatggtc cccgtgtcgg cgctggtcga
     1981 acgggtggtg gtcgacgccg ggccggacat cgccgatgac tggatcgccg agcgggccgc
     2041 gcccggggat gtggtggtga ccaacgacat cccgctggcc gaccgcgtgc tcaaggccgg
     2101 cgccacggcg atcaagccga acggccagcc gttcaccgcc gactccatcg gcggcgccct
     2161 ggcccagcgg gcgatcatgg agcacatccg ctcgaccggc gagatcaccg gcggcccccg
     2221 gccgttcgac cgcaacgacc gctcgcgctt ccttcaggcc ctcgacgaag cgatccagcg
     2281 cgagaagcgg cggcggcgat gatcgagatc acccccgcca tcgccctcga cgagtccgag
     2341 ctggagttcg cctacatccg cgccggcggg ccgggcgggc agaacgtcaa caaggtcgcg
     2401 acagccgtgc agatgcgctt cgacgcgcgg cgctcgccct cgttgcccaa cgacgtctcg
     2461 acgcggttgc agaagctggc cgggtcccgg ctgacgctgg acggggtgat cgtcatcgcc
     2521 gccagccggt tccgcaccca ggagcgtaac cgggccgacg ccatcgaccg gctggtcgag
     2581 ctgatccgca aggccgccga accgccgccg ccgccacgca agaagaccag gccgtcgaag
     2641 gccgcgaagg cccgccgggt cgacgccaag acccggcgcg gcggcatcaa ggcgatgcgg
     2701 agccgtccgg gcggcgacga ctgaggtcga ggggacaggt accctctcgg gtacgtgtcc
     2761 cccaatcgag tgatcgcagg gttaggggac acgcaccttc ggtacatgtc gcccgactac
     2821 tccagccaga acagcgcctc gaccggctcg cccagcgcct tcgccagccg cagggcgagc
     2881 acggtcgagg gcacgaacac gccgttctcg acggtgttga tggtcttgcg ggtcacgccc
     2941 acccggtcgg ccaggtcggc ctgggtaagc cccagccggg tccgcgccgc cttcaggccg
     3001 ttggcgagcc tcgggtcagc catgacgttc ggcgcggcgc tccagcgcga cgaagacgat
     3061 agccggagtc accacgccgg cggtcagcag cgcgtgaacc gcttcaagcg cggtcatggg
     3121 gacaaacagc gacaggacgt aaaggcccag cgcgaggatc atcaccaccc agaagccggt
     3181 gaccagggcc gtgcgccggt gagcgcgggt gctctcgtcc tcgaggatcg cgcgcaacgc
     3241 cctgttgcgg aaccagccgc cgccggtgga cagcagcagc agcagcgccg cgccccagac
     3301 cacgaagccc gacagcttga cctggtcgac ggtgcgcagc ggcgcttcca gcgtccacag
     3361 gccgttgaga aaggtcccct gccagacaag gaagaacaga ccctggaccc agatcagccg
     3421 agtccgacgc ctctgcgcgc gctcgacccg ctcggacagg gacggttcgg gtttgccttt
     3481 gccggccatg gcggcctcct atgtaacgca caggttacat gtaaccttga cgttacacac
     3541 cgtcaagccc ccgtgcggca ggaccggttg cgctggatca attgctccgc ggcgccgttg
     3601 catccgccga aaaccgcgct agcgtgcgcc cgcatcagtc gggagaattg gttatgcgcg
     3661 ctgaagtcaa agtcctcgtc ctggtcggcg ccgccgcgct tctgaccgcc tgcagcaagc
     3721 cggccgagac cccggccgcg acggaagccg tccccgccga cagcgccgcg acgcccgccg
     3781 cgacaccggc tcccgccgcg aaagccgaga gcgaggagtc cgacgaacat gccgagaagg
     3841 tgctcgcctc cctccccgcg ccctacaaca ccgccgacct ggagaacggc cagcgcaagt
     3901 tcgccctctg ccggtcgtgc cacaccatcg tcgagggcgg cccgaacatg accggtccga
     3961 acctgtacgg cgtgttcgga cgcaccgccg gcacggcgcc gaagttcaac tattccgacg
     4021 ccgtgaaggc cgccggcttc aagtgggacg ccgcgcatct ggacaagtgg ctggccgatc
     4081 cgcgcgccga ccttccaggc aaccgcatga ccttcgtcgg cctgaaggat cccaaagacc
     4141 gcgccgacgt gatcgcctat cttatggtgg aaacggccag gaagtccgac gacgactgac
     4201 ggagcgccat gggcgatacc gaagccctgc tgacccgcct ctacgaggcc ttcaaccgca
     4261 aggacatcga ggcggtcatc agcgccctgc acccggacgt cagctggccg aacctgtttg
     4321 gcgagggtcg gctgcaaggg cacgcggcga tgcggcgcat gtggcgcgag cagttctcga
     4381 agatcgatcc ggaggcgacg ccgatccgct ttctgcgcca ggaggacggc agcctcaccg
     4441 tgctgatcag ctatgtcgtg cgcaccctcg acggcaaggt gttcaccgag gagatcgcca
     4501 ccaacaccta tcggttcagc gacggcaagg tgatcggcat ggagtgggcc tgacacgaag
     4561 attcgactgg ccaccctgtc cgaccggtgg aaaggtcgga aacacgacat acgccgggcc
     4621 ggagggtgac gtgcgatgga tgaccgtcaa gacaccatcg atcgactctg ggacgccctg
     4681 aaccggcagg acatcgacgc tgcggcggcg ctgctgcacc ccgatgtcga ctggcaggac
     4741 ctgatgaacg gcggccggcg gaccggtgtg gccgatgtgc ggctctactg ggcggacctg
     4801 ttcaggatca tgcgccccga gtccacctcc ctgactttca ccccgaccgg ggacggccgc
     4861 gtggcggtgc ggacgcacca cagcatccgc gacccgtcgg gcaggttctg gaccgaggag
     4921 accatcaccc atctgttcgg cttcacggac ggcctgctca gcagcatgga tgtcgcctga
     4981 cgccggtcag accatcagat cagcgcaggc atcgaggatc gcgccggggt cgaggcggta
     5041 tttcgcatag aggtcgggca ggtcgccgga ctggccgaag gtctccaggc cgagggcccg
     5101 gacgcgctgg ccgcgcacgg atccgatcca cgacagggcc gccggcgaac cgtcgatgac
     5161 cgtcaccaga ccggccgcgg ggtccagatc ggccagcagg gtctcgatcg tcgccgtgcg
     5221 ctgcgcgccc gtcgtccagc gggcccgtcc ggcggcggtc cagtcggcat gcagcacgtc
     5281 cggcgaggtc acggcgagca ggccggcgcc cggctcgtct tccagcagtt gctcgaaggc
     5341 ggccagggct tctggcgcga tagccccgct gtagaccagg gccagccggg cgtcgggcgc
     5401 cggccgtttc atccagtagg cccccgccac gcagcccgcg cgccaggcgt cgtcggcccg
     5461 ggtcggctga tcgatgaccc gcgtcgacag ccgcaggtag gtcgagccgc cctccgacgc
     5521 ctggatgcgc gcgaacgcat gggccatcag gacggccgtc tcgtcggcga aggccggttc
     5581 gtagctgtcc aggcccggct gggccatgcc gatcaggggt gtgccgatcg actggtgcgc
     5641 cccgccctcg ggcgccaggg tcaggccgga cggcgtcgcc accagcagga agcgggcgtc
     5701 ctggtagcag gcgtagttca gcgcatcgag gccacgggcg atgaatggat cgtagagggt
     5761 gccgaccggg aacagccgct cgccgaacag cgggcccgac aggccgaggg ccgccagggc
     5821 gatgaacagg ttgttctcgg caatgcccag ctcgatgtgc tggccggtct cgcccatggc
     5881 ccagacctgg gccgagggga tgcggcggtt cttgaacacg tcctcatggg cccggcgatg
     5941 gaacacgccg cgccggttga cgaaaccgcc gaggttggtc gagacggtca cgtccggcga
     6001 cgtggtcacg acccggccgg cgaattcgtc gacgcccttg gcgatctcga acatcacctt
     6061 gccgaaggcg gcctgggttg actgctcgcc gccgccctcg acggccttga gcagggtctc
     6121 ggccgtcggc gtggcgacgt tcggcgcgtc gcgatggccg tccttgcgcg cggcgaaggg
     6181 ggccgtctcg accagccgct tcatcgcctt cagctcgttg tcggccaggc cggagaagac
     6241 atcccactcc tccccctcgc gcacgcccag ccgcgcgcga agctccgcga tctgcgtcgg
     6301 ggtcatcagg ccggagtggt tgtccttgtg gccggcgaac ggcagccgcc agcccttgac
     6361 cgtgtaggcg atgaagaagc gcggggcgtc gtcctcggcg gccttggcga aggcttcgag
     6421 gatggtttcc aggcagtgac cgcccagctc ggtcatcacc agggcgagct cggtgtcgtc
     6481 gtatccgccg atcagtttca gcgccgcctt gtcgcccgcg agatcgacca tcagacgctc
     6541 gcgccaggcc gccccgccct ggtaggtcag ggcggaatag aggtcgttgg gcgcgctctc
     6601 gatccaggcc tgcagcgcag cgccgccggg tcgggcgaag gcctgctgct ggcgcctcga
     6661 ccacttcagc gtctcgacct tccagccggc cgcctcgaaa atctcgccgt agcgggtgaa
     6721 catccggtcg ctggtcgtcg cgtcgaggct ctggcggttg tagtcgacaa tccaccaggt
     6781 gttgcgcacg tcgtgtttac aggcctcgat cagggcctcg tagatgttgc cctcgtcgag
     6841 ctcggcgtca ccgagcaggc tgatcatccg gcccatggcg tcgggtttca ccgccccgcg
     6901 ggcggccaga tagtcctggg tcaggctggc gaaggcggtc atggccacgc cgaggccgac
     6961 cgagccggtg gagaagtcga cgtcgtcgat gtccttggtc ctggagggat aggactgggc
     7021 gccgccgagc gcgcggaacc gctgcagctg gtcgatcgtc tggcggccgt agagatgctg
     7081 gatggcgtgg aacaccgggc tggcgtgcgg cttgaccgcc acccggtcct gcggccgcag
     7141 ggcgctgaaa tagagcgccg tcatcagggt ggtcatcgac gcgcacgacg cctggtgacc
     7201 gccgaccttc aggccgtcgc ggctgtcgcg aaggtggttg gcgttgtgga tcgtccagct
     7261 ggccagccag agaacccgtt cctccagacg acgcagaagc gcgatatcct tgccgcgaac
     7321 agcgaaaccg gcgtcgtcca tgtccacctt cacctattcg gcgggtgaag cctgccccgc
     7381 cgtctcgccc tgctcatacg cctcgatctg cctggccgtc cactccggcg acttggtgaa
     7441 gcgggccagc atgttgtaga agaccggcac gacgaacacc gtcagcaggg tggcgaagat
     7501 cgccccgaaa aagatcgtca ccccgatcgt ctggcggctg gccgcgccgg cgcccgacca
     7561 caggatcagg ggcagcgagc cgaaggcggc ggtgatcgag gtcatcacga tcggccgcag
     7621 gcgcaagacc gccgcctcga tcaccgcgtc gtggatcgac cggccctgat cgcgcagctg
     7681 gttggcgaac tcgacaatca ggatgccgtt cttggccgcc accccgatca ggatgatcag
     7741 accgatctgg ctgtaggtgt tgatcgtctg accggtgagc agcaggccga acagtccgcc
     7801 cagcgccgcc agcggcacgg tcagcatgat caccgccggg tggatccagc tctcgaactg
     7861 cgcggcgagc accaggaaca ccagcaacag ggccatgccg aaggccaggc cgaccgcgcc
     7921 cgacgcctcg agatagtcct tggcctgacc gtcccaggtg taggagaccc cggccgtcgg
     7981 ccgcttggcg gcttccgcct ggaggaattt gaccgcctcg gcgacggtga agcccggagt
     8041 cagctgactc gttaggcgca ccgagcgctg gcggtcggtg cggttgcggt cgggcgtgtc
     8101 accgcgcagc tgtgtcgtga ccaccgccga aagcggcacg agctggcccg agccggcccg
     8161 cacatagagg gtattgaggt cggccagggt gcggcgcttc tccaggtcgg tctggacgat
     8221 gacgtcgtat tcctgccccc ccttggtgta ggtggtcacc cggcgggagc cgaacatggt
     8281 ctccagcgac cggccaatgg cctggggcgt cacgccgagc gaggcggcct tttcccggtc
     8341 gatctcgacc gacaggcgcg gcgcggtcgg ctcgtagtcg agccgcgggc gggagaagcc
     8401 gggattgcgc atcatggcgt cacggatcgg ccccagccag cgggcgatct cgtcgtagtc
     8461 gttgcccagg ccgaccaggt cgacgttgct gccaccgccg ctgccgccgc cgcgctggaa
     8521 ggcgccgcgc acgctggcca cggcgcgcac cccggtaatg gcgccaagag acttgttcag
     8581 ctcatcagcg acctggtcgg aggtcttggt ccgctcgccc cagggcttga gcacgagcac
     8641 ggcgttgccc gtgttgaact gggtggcgcc gaaccgcggg atgaccacca catagcgttc
     8701 cgcctcgccc tgctcgtaat agcggcgaac ctggcgttcg acctggttgg cggccttgag
     8761 ggtgtagtcg aagccggcgc cctcgggccc ggagatcgag acatcgaccc ggccccggtc
     8821 ctcggccggc accagctcct ggggcaggac catgaacagg gttcccgcga gggccgcgag
     8881 ggccagcacc agggccaggg cgccgccgcc cgcgatccgg ctggccagca gcatctccag
     8941 ggagtgccga taggagtccc ggacccgctc gacgacgaga tccacccggc tggccagcca
     9001 gccattgccc gatgccggcc gcagcagcct tgaggccagc atcggcgaca gggacagggc
     9061 caggaaggcc gagaaggcca ctgccg
//
LOCUS       DAORNX010000061         9055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5683_length_9055_cov_7.829111, whole genome shotgun sequence.
ACCESSION   DAORNX010000061 DAORNX010000000
VERSION     DAORNX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_9055_cov_7.829111"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLO65_04200"
     CDS             <1..391
                     /locus_tag="PLO65_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HRD27484.1"
                     /translation="WRGVLIEGPSGGGKSDLALRALSEGFRLVADDRVLVWASGGQVW
                     GRAPGPLAGLMESRGLGVLPVNAIAFARIVLAVHCAASPAAVERSPADETLDLAGIAV
                     PLRRLWPFDPAAPAKLRRSMEYLGAEH"
     gene            480..884
                     /locus_tag="PLO65_04205"
     CDS             480..884
                     /locus_tag="PLO65_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRD27485.1"
                     /translation="MIGLVIVTHGRLAEEFVFAMEHVVGPQTDVAAICIGPDDDMEKR
                     RKDILKACAAVDSGKGVILLTDMFGGTPSNLAISVMEQTRAEVIAGLNLPMLIKLASL
                     RTREPLDACVHHAQEAGRKYISVASYVLAGEK"
     gene            881..1153
                     /locus_tag="PLO65_04210"
     CDS             881..1153
                     /locus_tag="PLO65_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRD27486.1"
                     /translation="MSVSRTVEIVNERGLHARASAKFVKLASGFDAEVSVSRDGSTVD
                     ARSIMGLMMLAAGIGSTIEISAEGAEAAQAVAALCELVENRFDEER"
     gene            complement(1589..2590)
                     /gene="bioB"
                     /locus_tag="PLO65_04215"
     CDS             complement(1589..2590)
                     /gene="bioB"
                     /locus_tag="PLO65_04215"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRD27487.1"
                     /translation="MNAPVRFADPALPRHDWTREEIEALFDLPFTELVFQAAGVHRRW
                     FDPTELQLSQLLSVKTGGCAENCGYCSQSAFFKTGLQAEKLMAFEDVLTAAKKARDGG
                     AQRFCMGAAWRDLKDRDLPRVAEMITGVKALGLETCVTLGMLKAEQAVALKDAGLDYY
                     NHNLDTSPEYYDKVVTTRTYDDRLETLQHVRDAGISTCCGGIVGMGESRRDRASFLHQ
                     LAILPVHPDSLPVNALVPIAGTPLGDKVKRDGEIDPLEFVRTVAVARLVCPKSMVRLS
                     AGRDDMSRETQALCFLAGANSIFIGGKLLTTPLPGQDEDSLLLKDLGMKPMSVPQVS"
     gene            2772..4163
                     /gene="ahcY"
                     /locus_tag="PLO65_04220"
     CDS             2772..4163
                     /gene="ahcY"
                     /locus_tag="PLO65_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRD27488.1"
                     /translation="MTDSIVRDIALADYGRKEIAIAETEMPGLMALRHEFGKDQPLKG
                     ARIAGSLHMTIQTAVLIETLKALGADVRWASCNIFSTQDHAAAAIAANGTPVFAIKGE
                     TLYEYWQYTHKIFEWSDGGYPNLILDDGGDATLLTVLGPRAEKDPSILDKPENEEEEA
                     LYAVMKTYLKEKPGFYSAIRAAITGVSEETTTGVHRLYQLAARGELPFPAINVNDSVT
                     KSKFDNLYGCRESLVDAIRRGTDVMLSGKVAVVCGYGDVGKGSAASLRQGGARVIVTE
                     IDPICALQAAMEGYEVQTLDDVCDKSDIFVTATGNKDVITVDHMRRMRNNAIVCNIGH
                     FDSEIQVAGLKNFKWDEIKPQVHHIEFPDGKKIILLSEGRLVNLGNATGHPSFVMSAS
                     FTNQTLAQIELWTNLKAYEHQVYTLPKHLDEKVAALHLEKLGAKLTVLRKEQADYIGV
                     PERGPFKPDHYRY"
     gene            complement(4167..4418)
                     /locus_tag="PLO65_04225"
     CDS             complement(4167..4418)
                     /locus_tag="PLO65_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27489.1"
                     /translation="MSKTSYPLKLPTSVKTAAQRLAREDGVSLNQWISSAVAEKVGAV
                     ETSSAFLKRRSAGAKPEDLLWFLDNAANEPPIPEDERLA"
     gene            complement(4415..4840)
                     /locus_tag="PLO65_04230"
     CDS             complement(4415..4840)
                     /locus_tag="PLO65_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRD27490.1"
                     /translation="MLNVVLDTSVVVAALRSRTGASNALLRHVARRELAPLATTALFL
                     EYEDVLRRPEQRLVHGLDLDQIDRFLAALASACEAVDVRFQWRPQLSDPNDEMVLEAA
                     VNGRADRLVTHNVRDFASAGVKFGLPVVRPGALLLELRP"
     gene            complement(5073..5483)
                     /locus_tag="PLO65_04235"
     CDS             complement(5073..5483)
                     /locus_tag="PLO65_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27491.1"
                     /translation="MTRIVFGIAAIVSAAVCAIGLFGLPIYFGALAGSGTDIGADDVG
                     ALILMIALLILELISAGTGFLSLLTESARAFRWTAVLALLGAAIFAAGVAVSYGVNIH
                     RLGFTELLMAWWLVWAGLAAAEFVRLRKSRPTKG"
     gene            5620..6471
                     /locus_tag="PLO65_04240"
     CDS             5620..6471
                     /locus_tag="PLO65_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD27492.1"
                     /translation="MPMALIMSSHVAGSRVGGSAQALALTQFKIDPVVVPTVLYGRHP
                     GWGAPGGAQVPIEAFEGMLDGVEANGLFGMTDLVLTGYFAGVEQVKAAARAIDAVKAA
                     PRDGAFARKPMVVVDPVIGDSGKGLFVPAEVAQAVIDHLIPRADLVAPNSWELQRITG
                     ADARSPEAAAKAARLLGKAVLVSSVVRGAEIGVVYADRREAWLAAHQKTGETVPSGTG
                     DLLTALFAAALLEGQTPSYGLARAVGGLAETIASAGIWASPELPIVAMGARIKQASPT
                     VRIERLA"
     gene            6471..7595
                     /locus_tag="PLO65_04245"
     CDS             6471..7595
                     /locus_tag="PLO65_04245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27493.1"
                     /translation="MTEIHGVCEPRFEAVRDAFAANFAEGKELGARFAFSVEGEIVVD
                     LWAGWADRKLTVPFDARTLTPVYSTTKAVAATMIARLVEQGALRWNQPVAEVWPEFAA
                     EGKGAVTVEQMISHQAGLPGFVEPIDPTLWYDPPVIAAMLAAKAPMWPVGSASGYHAL
                     TIGYLVGELFRRVDGRTLGTALREDIAGPAGLDLWIGLPDSEHGRCADLKPPSAMPQF
                     GEVTDPKTAAFLSPWAAPSGRGSADWRRIEIPSANGHTTAEALARFMGALASDGEVDG
                     VHILSPASVAEAGRERIRGQDLVLPFDLSWAAGFMRNVPNHFFGPGEATIGHSGWGGS
                     CAFADPQHRVAGAYVMNRQDVHLIGDPRSMRLIEAAYASL"
     gene            complement(7634..>9055)
                     /locus_tag="PLO65_04250"
     CDS             complement(7634..>9055)
                     /locus_tag="PLO65_04250"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27494.1"
                     /translation="PGLARAGSPPVQGIAAEVVALLEPSAAKAERRRFWETRLSSAAR
                     NRWPWTVFDQDIAALAVASGGAALLSVEPGVAPGGFSLGLRTRKQGLVRFIRLRIDRD
                     EPDRLFEVRAMPSPTPYPRADLPTGPISREDLAAAIDRRLRFAVDRDEFSGAVRVLAP
                     DGAVVYEQAFGMADKAAGVANTSTTRFHIGSADKSFTAIMAGRLIEAGGLSLDTPLVD
                     ILPDYPNREAAAAITVRHLLTHSSGLGGLFDRPGFDGKKPFRRMADLFPAFASEPLAF
                     APGSRAAYSNEGFVVLGAVIEKVTGESWYDLLARQVYRPAGMTRSGHPILGEPATGMA
                     IGYQYGDNDPLALAGRSRNDGRRGYRGNSCGGGYCTVGDMTAYIVALRAGRLLPPPML
                     QTMTRQAEGGLRDYGMGFQIRSVGDRRTIGHGGGGPFSGIDGDTRIVWETGWAWSILG
                     NYDAPFAGEVSADIATLLAAQSG"
BASE COUNT         1456 a         3158 c         3084 g         1357 t
ORIGIN      
        1 ctggcgcggc gtgctgatcg aggggccgtc cggcggcggc aagagcgacc tggccctgcg
       61 cgccctgtcc gaggggtttc gcctggtggc ggacgaccgg gtgctggtct gggcctccgg
      121 cggccaggtc tgggggcggg cgcccggtcc gctggccggg ctgatggaga gccgcggcct
      181 gggcgttctg ccggtcaacg ccatcgcctt cgcccgcatt gtcctggccg tccattgtgc
      241 ggcgtcgccg gccgcggtcg agcgaagccc ggccgacgag acgctcgatc tggccgggat
      301 cgcggtgccc ctgcgccgcc tgtggccttt tgatcccgcc gcacccgcga aattgcgccg
      361 cagcatggag tatcttggag ccgaacatta acgggcgtat ctaggcgcct tcgcggcgag
      421 ggccgcgtcc gtcgggcggg ggttcccggt ttgatcgaga cagggtgtca ggctcgcgga
      481 tgatcggcct cgtgatcgtt acccatggcc ggctggcgga agagttcgtc ttcgccatgg
      541 agcatgtcgt cggtccgcag accgacgtgg cggccatctg catcggcccc gacgacgaca
      601 tggaaaagcg ccgcaaggac atcctcaagg cctgcgcggc cgtcgattcg ggcaagggcg
      661 tgatcctgct gaccgacatg ttcggcggca cgccgagcaa cctggccatc agcgtcatgg
      721 aacagacccg cgccgaggtc atcgccgggc tgaacctgcc gatgctgatc aagctggcct
      781 cgctgcgcac gcgcgagccg ctggacgcct gcgtgcatca cgcccaggag gcgggccgca
      841 agtacatctc cgtcgcctcc tacgtgctgg ccggcgagaa gtgagcgtca gccgcacggt
      901 cgagatcgtc aacgagcggg ggctgcacgc ccgcgcctcg gccaagttcg tcaagctggc
      961 ctcgggcttc gacgccgagg tgtcggtgtc gcgcgacggc tcgacggtcg acgcccgctc
     1021 gatcatgggc ctgatgatgc tcgccgccgg catcggctcg accatcgaga tcagcgccga
     1081 gggcgccgag gcggcgcagg cggtggcggc gctgtgcgaa ctggtcgaga accgcttcga
     1141 cgaggagcgg taggggggcg aattagagcc gtttcccctg ttccattcgt tcgtggagta
     1201 tacggaccgc cacgatcccg gccgcatgtt ccctgaagaa caggatgtgg cggccgaccc
     1261 gtagtctgcg catgtccggc cgatcagatc tgacggtggg atagcgggcg ccccttgcgg
     1321 tcacatgggc gcagggagcg atccgcggac cgacccccgt tttcataggg ccgggcacct
     1381 actgggtcgt ctgggtgttg ccccactcca cgtcatcctg agcaggcccg cagggccgtg
     1441 tcgaaggacg cacgatcagc ccgcgtggtt cgacacgcgc ttcgcgctgc tcaccatgac
     1501 gtggatcgag cctggcggcc ccacctccac ccccatcccg agccttcccc cctccagggg
     1561 gcgggctccc gcaaggggag aaggacggct acgagacttg cgggacgctc atcggcttca
     1621 tccccaggtc cttcagcagc aggctgtcct cgtcctggcc gggcaggggg gtggtcagca
     1681 gcttgccgcc gatgaagatc gagttggcgc cggcgaggaa gcacagggcc tgggtctcgc
     1741 ggctcatgtc gtcgcggccg gcggacaggc ggaccatgga tttcggacag accagccgcg
     1801 ccacggcgac cgtgcgcacg aactccagcg gatcgatctc gccgtcccgc ttgaccttgt
     1861 cgcccagcgg cgtgccggcg atcggcacca gcgcgttgac cggcaggctg tcgggatgga
     1921 ccggcaggat ggccagctgg tgcaggaagc tggcccggtc gcggcggctc tcgcccatgc
     1981 cgacgatgcc gccgcagcag gtcgagatgc ccgcgtcgcg cacatgctgc agcgtctcca
     2041 gccggtcgtc gtaggtccgg gtggtgacga ccttgtcgta gtactccggc gaggtgtcga
     2101 ggttgtggtt gtagtagtcg aggccggcgt ccttcagggc cacggcctgt tcggccttga
     2161 gcatgcccag ggtcacgcag gtctccaggc ccagagcctt caccccggtg atcatctcgg
     2221 ccacgcgcgg caggtcgcgg tccttcaggt cgcgccaggc ggcgcccatg cagaagcgtt
     2281 gcgcgccgcc gtcccgggcc ttcttcgcgg cggtcaggac gtcctcgaac gccatgagct
     2341 tctcggcctg gaggccggtc ttgaagaagg ccgactggct gcagtagccg cagttctcgg
     2401 cgcagccgcc ggtcttgacc gacagcagct gcgacagctg cagctcggtc gggtcgaacc
     2461 agcgccggtg gacgccggcg gcctggaaca ccagctcggt gaacggcagg tcgaacagcg
     2521 cctcgatctc ctcgcgcgtc caatcgtggc gcggcagggc gggatcggcg aaacggacgg
     2581 gcgcgttcat gcgggcataa ctccggttgg cgggagcctt ctagccaacc tgccgcgtca
     2641 aggcgacccg gaatgggtcg ggatttgcga ctggccgccc ggggacataa agatatcttt
     2701 atgtcgacgt tgcggcggcg ccctgcggcg gctatagagc cccccagaca acgacggaca
     2761 ggagtccccg cgtgaccgac agcatcgtgc gcgacatcgc gctggccgac tatggccgca
     2821 aggaaatcgc catcgccgag accgagatgc ccggtctgat ggcgctgcgc cacgagttcg
     2881 gcaaggacca gccgctgaag ggcgcgcgga tcgccggctc gctgcacatg accatccaga
     2941 cggccgtgct gatcgagacg ctcaaggccc tcggcgccga cgtgcgctgg gccagctgca
     3001 acatcttctc gacccaggac cacgccgcgg cggccatcgc ggccaacggc accccggtgt
     3061 tcgcgatcaa gggcgagacg ctctacgagt actggcagta cacgcacaag atcttcgaat
     3121 ggtccgacgg cggctatccg aacctgatcc tcgacgacgg cggcgacgcc accctgctga
     3181 ccgtgctcgg cccgcgcgcg gagaaggacc cctcgatcct cgacaagccc gagaacgagg
     3241 aggaggaagc cctctacgcg gtgatgaaga cctacctgaa ggaaaagccg ggcttctatt
     3301 cggcgatccg cgcggcgatc accggcgtct ccgaagagac caccacgggc gtccaccgcc
     3361 tgtaccagct ggccgcccgg ggcgaactgc cgttcccggc gatcaacgtc aacgacagcg
     3421 tgaccaagtc caagttcgac aacctctatg gctgtcgcga gagcctggtg gacgccatcc
     3481 gccgcggcac cgacgtgatg ctgtcgggca aggtcgcggt ggtctgcggc tacggcgatg
     3541 tcggcaaggg ttcggccgcc tcgctgcggc agggcggcgc ccgtgtgatc gtcaccgaga
     3601 tcgacccgat ctgcgccctg caggcggcga tggaaggcta tgaggtccag accctcgacg
     3661 acgtctgcga caagtcggac atcttcgtca ccgccaccgg caacaaggac gtgatcaccg
     3721 tcgatcacat gcgccgtatg cggaacaacg ccatcgtctg caacatcggc cacttcgaca
     3781 gcgagatcca ggtcgccggc ctgaagaact tcaagtggga cgagatcaag ccgcaggtcc
     3841 accacatcga gttcccggac ggcaagaaga tcatcctgct gtccgaaggc cggctggtga
     3901 acctgggcaa cgccacgggc cacccgtcgt tcgtgatgag cgccagcttc accaaccaga
     3961 ccctggccca gatcgaactg tggaccaacc tcaaggccta cgagcatcag gtctacaccc
     4021 tgcccaagca cctcgacgaa aaggtcgccg ccctgcacct cgagaagctc ggcgcgaagc
     4081 tgaccgtgct gcgcaaggaa caggccgact acatcggcgt ccccgagcgg ggcccgttca
     4141 agccggacca ctaccggtac taggcgtcag gcgagccgct catcctccgg gatgggcggc
     4201 tcgttggcgg cgttgtcgag gaaccacagc aggtcctcgg gtttcgcgcc cgccgacctg
     4261 cgcttcagga aggcgctgct ggtttcgacc gcgccgacct tttccgccac ggcggagctg
     4321 atccactgat tgagcgaaac gccgtcctcg cgggccagcc gttgggcggc ggtcttgacc
     4381 gacgtgggca gtttcagcgg gtaggaggtc ttgctcatgg cctcagctcc aacaacaacg
     4441 cgcccggcct gaccacgggc agtccaaatt tcacgccagc gctcgcgaag tcccgaacat
     4501 tatgggtgac gagccggtcc gcccggccgt tgacggcggc ctccagaacc atctcgtcgt
     4561 tcgggtctga cagctgcggc cgccactgaa accgcacgtc gaccgcctcg caggcgctgg
     4621 ccagcgcggc caggaaccgg tcgatctgat ccaggtccag accatggacc aaccgctgtt
     4681 ctggccgccg aaggacatct tcgtactcaa ggaacagagc tgtggtggcc agcggcgcaa
     4741 gctcccgtcg cgcgacgtgg cgcaggagcg cattgctcgc gccggtccta ctccgcaggg
     4801 cggcgacaac caccgatgtg tcgagaacga cgttaagcat atctcagaag atatcatatc
     4861 tctcgagatg tcgcaatttg tggtgaacgg cgtgcctgcg cgagacagtc ctgcgcaagg
     4921 accaggccga ctacatcggc gtccccgagc agggccggtt caagccggac cactaccggt
     4981 accaggacgg ggcgcttcgg cgatgagacg accgcggtga ccatgcggcc accgcccttt
     5041 ttttgtagcg cgtcatcagt gttcgccggg cttcagccct tcgtcggccg cgacttccga
     5101 aggcggacga actccgcagc ggccaggccg gcccagacaa gccaccatgc catgagcagc
     5161 tccgtaaagc cgaggcggtg aatgttgacg ccgtaggaga ctgccacacc cgccgcgaaa
     5221 atggccgcgc caagcaacgc caggacggct gtccatcgaa aggcgcgcgc actctctgtc
     5281 aggagcgaca agaaccccgt tccggcggaa atgagttcaa ggatcaggag cgcgatcatc
     5341 agaatcaacg cgccgacatc gtccgcgcca atgtcagttc cactgcccgc tagcgccccg
     5401 aaatagatcg ggagcccaaa caggccaatc gcacagacgg ccgccgaaac gatggcggcg
     5461 ataccgaaga caatcctcgt caactgccgt ctccaacccg gtgagcgccg cagccggcat
     5521 cagtccgcac gcgcaactca ggccgaagcg gattttcagt caaagggaga attcgtgcac
     5581 gactagcgca gacctcccac gtccgctaat ccccctccca tgccgatggc cctcatcatg
     5641 tcgagccacg tcgcggggag ccgggtcggc ggctccgccc aggcgctggc cctgacccag
     5701 ttcaagatcg atccggtggt cgtgcccacc gtcctctatg gccgccaccc cggctggggc
     5761 gcgcccggcg gggcgcaggt gccgatcgag gccttcgaag gcatgctcga cggagtcgag
     5821 gccaacgggt tgttcggcat gaccgacctg gtcctgaccg gctatttcgc cggcgtcgaa
     5881 caggtcaagg cggccgcccg ggcgattgat gcggtcaagg ccgcgccgcg cgatggcgcc
     5941 ttcgcgcgca agccgatggt cgtcgtcgat ccggtgatcg gcgattccgg caagggcctg
     6001 ttcgtgccgg cagaggtggc ccaggcggtg atcgatcatc tgatccccag agccgatctg
     6061 gtcgcgccca acagctggga gctgcagcgg atcacgggcg ccgacgcccg cagccccgag
     6121 gccgcggcca aggccgcgcg actgctcggc aaggcggtgc tggtctcctc ggtcgtgcgc
     6181 ggcgccgaga tcggcgtggt ctatgccgat cgccgggagg cctggctggc cgcgcaccag
     6241 aagaccggcg agaccgtgcc cagcggcacg ggcgacctgc tgaccgccct gttcgccgcc
     6301 gccctgctgg aaggccagac gccgtcctac ggcctggccc gcgccgtcgg cggtctggcc
     6361 gagaccatcg cctcggccgg catctgggcc tcgcccgagc tgccgatcgt ggccatgggc
     6421 gcccggatca agcaggcgag ccccaccgtc cgtatcgaga gactggcctg atgaccgaga
     6481 tccacggcgt ctgcgaaccc cgcttcgagg ccgtccggga cgccttcgcg gccaacttcg
     6541 ccgagggcaa ggagctgggg gcgcggttcg ccttctccgt cgagggcgag atcgtcgtcg
     6601 acctgtgggc cggctgggcc gaccgcaagc tgaccgtgcc gttcgacgcg cgcaccctga
     6661 cgcccgtcta ttcgacgacc aaggccgtgg ccgcgacgat gatcgcccgg ctggtcgagc
     6721 agggcgccct gcgctggaac cagcccgtcg ccgaggtctg gccggagttc gcggccgagg
     6781 gcaagggcgc ggtgaccgtc gagcagatga tctcgcacca ggccggcctg ccgggcttcg
     6841 tcgagccgat cgatccgacc ctctggtacg acccgccggt gatcgccgcc atgctggccg
     6901 ccaaggcccc gatgtggccg gtcggctcgg ccagcggcta tcacgccctg accatcggct
     6961 atctggtcgg cgagctgttc cgccgcgtcg acggccgcac tctggggacc gccctgcgcg
     7021 aggatatcgc cgggcccgcc ggcctcgacc tgtggatcgg cctgcccgac agcgagcacg
     7081 gccgctgcgc cgacctcaag ccgccctcgg ccatgccgca gttcggcgag gtcaccgacc
     7141 cgaagacggc cgccttcctg tcgccctggg ccgcgcccag cggtcgcggc tcggccgact
     7201 ggcggcggat cgagatcccc tcggccaacg gccacaccac ggccgaggcc ctggcccggt
     7261 tcatgggcgc cctggccagc gacggcgagg tcgatggcgt gcacatcctc tcgcccgcct
     7321 cggtcgccga ggccggccgc gaacgcatcc gcggccagga cctggtgctt ccgttcgacc
     7381 tgagctgggc cgccggcttc atgcgcaacg ttccgaacca cttcttcggc ccgggcgagg
     7441 cgaccatcgg ccactccggc tggggcggca gctgcgcctt cgccgatccg cagcatcgcg
     7501 tggccggcgc ctatgtgatg aaccggcagg acgtccacct gatcggcgac ccgcgctcga
     7561 tgcgcctgat cgaggccgcc tacgcgagtc tgtaggcgga ggcgcgcacc accttcggta
     7621 cgtgcccccc tcgtcagccg gactgcgcgg ccagcagggt cgcgatgtcg gccgacacct
     7681 cgcccgcgaa cggcgcgtcg tagttgccca ggatcgacca ggcccagccg gtttcccaca
     7741 cgatccgggt atcgccgtcg atgcccgaga acgggccgcc gccgccatgg ccgatcgtcc
     7801 gccggtcgcc gacggaccgg atctggaagc ccatgccata gtccctcagc ccgccctcgg
     7861 cctgtcgggt catggtctgc agcatcggcg ggggcagcag cctcccggcc cgcagggcga
     7921 cgatgtaggc ggtcatgtcc ccgacggtgc agtagccgcc gccgcaactg ttgccgcgat
     7981 agccgcgccg cccgtcgttg cggctccgcc cggcgagcgc cagggggtcg ttgtcgccgt
     8041 actggtagcc gatggccatg cccgtcgccg gctcgccaag gatcggatgg ccgctgcggg
     8101 tcataccggc cggccgatag acttggcgcg ccagcaggtc gtaccagctc tcgccggtga
     8161 ccttttcgat gaccgccccc agcaccacga agccctcgtt gctgtaggcg gcgcgggacc
     8221 cgggcgcgaa ggccagcggc tcggacgcga aggccgggaa caggtcggcc atgcgtcgga
     8281 agggcttctt gccgtcgaac cccggccggt cgaacagccc gcccagcccc gaactatgcg
     8341 tcagcaagtg gcgcaccgtg atcgccgcgg cggcctcacg gttgggatag tcgggcagga
     8401 tgtcgaccag cggggtgtct agcgacaggc cccctgcctc gatgaggcga cccgccatga
     8461 tggccgtgaa gctcttgtcc gccgagccga tgtggaaccg cgtggtcgag gtgtttgcga
     8521 cgcccgcggc cttgtcggcc atgccgaagg cctgctcgta gacaaccgct ccgtcagggg
     8581 cgagcacccg gacagcgccg gagaactcgt cccggtcgac ggcgaatcgc agtcggcggt
     8641 ctatcgccgc ggccagatcc tcccgcgata tcggccccgt cggcagatcc gcgcgcggat
     8701 agggtgtcgg cgagggcatg gcgcggacct cgaacagccg gtcgggctcg tcgcggtcaa
     8761 tgcgcagccg gatgaaccgc accagcccct gtttgcgcgt gcgcagaccc agactgaagc
     8821 cgcccggggc gacgccgggc tcgaccgaca gcagggcggc gcccccggac gccacggcga
     8881 gcgcggcaat gtcctggtcg aagaccgtcc atggccagcg gttccgcgcg gcgctggaca
     8941 gacgggtttc ccagaagcgc cggcgttcgg ccttggcggc cgagggctcg agaagggcca
     9001 ccacctcagc ggcgatgccc tggaccggcg gcgatccggc gcgggcgagg ccggg
//
LOCUS       DAORNX010000062         8939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5780_length_8939_cov_7.784106, whole genome shotgun sequence.
ACCESSION   DAORNX010000062 DAORNX010000000
VERSION     DAORNX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_8939_cov_7.784106"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLO65_04255"
     CDS             complement(<1..162)
                     /locus_tag="PLO65_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD27495.1"
                     /translation="MLLPVDPARYVAFLTAMALMAVTPGPANLFAIAAGIQRGRRQAL
                     VGVAGMNAAT"
     gene            284..1075
                     /locus_tag="PLO65_04260"
     CDS             284..1075
                     /locus_tag="PLO65_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD27496.1"
                     /translation="MDGMGMGPDFWIFFAVGVAAQLVDGALGMAYGVVSSTVLLALGV
                     PPANASASVHAAKVFTGAASALSHIAYRNIGWKLLLHLSAGGVLGGVLGTYVLTSIDG
                     KVIKPWVVAWLGVMGLVILWRAWKGTRPRVMSWKRPGPLGLVGGFFDAVGGGGWGPVV
                     TSTLMGSGADPRQAIGTTNAAEFFVAVAISATFVGAIVSGHWDAIGLRDHVWSVGGLI
                     AGGVLAAPLAGWMTKVLPVRALTWLVGLLVTGLAIWQGATLAGLS"
     gene            complement(1072..1482)
                     /locus_tag="PLO65_04265"
     CDS             complement(1072..1482)
                     /locus_tag="PLO65_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD27497.1"
                     /translation="MQSYAYVAIVTLVALLVYFWMGLGVAGARRKCGIAAPCMTGDPV
                     LERSIRVQANTLEWLPIFLPSLWLFHLFWAPQDPMGQIAAGLGVVWIVGRILYALGYV
                     KDPGKRELGFLIQTLAAGVLLFGALGKAVLILLA"
     gene            1617..1823
                     /locus_tag="PLO65_04270"
     CDS             1617..1823
                     /locus_tag="PLO65_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HRD27498.1"
                     /translation="MRVESAAISDIRYDDDRGKLFVRFVDGDEYVYVGVPGEVHRSFV
                     EADSKGRFFAEQIRDRFPYNRLDS"
     gene            complement(1833..3071)
                     /locus_tag="PLO65_04275"
     CDS             complement(1833..3071)
                     /locus_tag="PLO65_04275"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRD27499.1"
                     /translation="MPAHKSVKKVVLAYSGGLDTSIILKWLQTEYGAEVVTFTADLGQ
                     GEEIEPARAKALAAGVKPENIFIEDVREEFVRDYVFPMFRANTVYEGQYLLGTSIARP
                     LIAKKQIEIARKTGADAVSHGATGKGNDQVRFELGYYGLEPDITVIAPWREWDFKSRE
                     ALLDFAEKHQIQITKDKRGEAPFSVDANLLHSSSEGKVLEDPAVEAPEFVHMRTISPE
                     DAPDKPHVFTIDFDKGDPVAIDGVAMSPATLLTTLNQLGHDNGVGRLDLVENRFVGMK
                     SRGVYETPGGTILLAAHRGIESITLDRGAMHLKDELMPKYASLVYNGFWFAPEREMLQ
                     AAIDYSQKNVSGQVRVKLYKGNVTVIGRTSPLSLYDQDLVTFEEGKVAYDHRDAGGFI
                     KLNALRLRVLAKRDQRAAKD"
     gene            complement(3132..3497)
                     /locus_tag="PLO65_04280"
     CDS             complement(3132..3497)
                     /locus_tag="PLO65_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27500.1"
                     /translation="MIHALSIVGDAFWIMSLALMCALSWGTWKRVGPGTPVPVAWKDG
                     VATTRAHRLPALWLVPALAIAAGAWLKFESRAPGLALDGAVIALGVRVTLAPLVAVLH
                     LSQLRRALATLEAEGQLKP"
     gene            complement(3502..5460)
                     /locus_tag="PLO65_04285"
     CDS             complement(3502..5460)
                     /locus_tag="PLO65_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRD27501.1"
                     /translation="MASEAPGASAPDKAASTPTTPNGQDGHAGHGFLALALGSIGVVF
                     GDIGTSPLYAMREALAHSKSGGATQLAVMGVVSLVIWAMFLIVTVKYVILLMRADNKG
                     EGGTLALMALARRTLSKQTGKRSAAVFFLGVVGAALFYGDGIITPAISVLSAMEGLKD
                     APGIGARVSPYILPISACILVALFMVQSRGTHRVASFFGPITLLWFLVLGGLGLFHLK
                     EDLSIFWAFNPWYGLRFLWENGFLGFVILGSVFLVVTGAEALYADMGHFGKGPIRASW
                     LVVVFPCLALNYLGQGSLILAHPETRHNPFWEMVPQFAYWPILLLAACATVIASQAVI
                     TGAFSVTQQAVQLGLLPRIDIRRTSETQAGQIFVPQVNTMLMIGVLVLLFSFGTSSAL
                     ASAYGIAVTGSMFVDTLLAFVIIKAAWKWTWPATIAVLAPLVALDLTFISSNLLKIPQ
                     GAWMPLVFGALLVLIMWTWTKGAQILTDKTRRDSVPLNDLIDILKARPPHRAPGTAIF
                     LTSDPDMTPVALMHNLKHNKVLHEKNVIATVRTAETPRVSEADRVRIEPVNDDFKKLI
                     ITYGFMETPNVPKALALLRKQQGLKFDIMATSFFLGRRSVIASAHSGMPLWQDKLFIF
                     LMRNAANPTDFFKIPPGRVVELGAQVTV"
     gene            5627..6058
                     /locus_tag="PLO65_04290"
     CDS             5627..6058
                     /locus_tag="PLO65_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRD27502.1"
                     /translation="MSDSQKFPVAAHALAYLAHKQATTAERAISSAELAASMPTNPVV
                     VRRVTAMLARAGLIETRAGAGGGAWLKVDPAGIALDTVLRAVGGCTHLGVAPPGAKGC
                     PVGELIPEAVSAAITAADTAASERLSRITVADLLKAPAEKL"
     gene            6143..>6742
                     /locus_tag="PLO65_04295"
     CDS             6143..>6742
                     /locus_tag="PLO65_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRD27503.1"
                     /translation="MTRIAIAYHSGYGHTEALAASVAQGVRDAGAEAILLRIDNAGQD
                     FDPLLDEITASDAVVFGAPTYMGDVSGVFKVFADATSKAFFTGAWKDKLAAGFTNSHS
                     FSGDKLHALTSLAILASQHGMNWVNLGVPAPNVPAAERGPETLNRVGAFLGLAGQSDN
                     AAAELSPPGGDRETARLLGVRVAEAARRWTQGAASEPARS"
     assembly_gap    6743..6853
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6927..7727
                     /locus_tag="PLO65_04300"
     CDS             6927..7727
                     /locus_tag="PLO65_04300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27504.1"
                     /translation="MPRRLALVTGASAGIGAAFARIYASHGYDVALTARRTDRLEALA
                     AEIRLRFGVEALVIAADLGEPGACDAILAEIESHGRVVDALVNNAGYGLAGAYADTRW
                     PDQRSMLQVMLVAVCELTHKVLPGMIDRRFGRIVNVASLAGLVPGSAGHTLYAATKSF
                     LVKFSQSLHLENLATGVHVSALCPGFTFSEFHDVNATRSQVSQATPEWLWLGADEVAA
                     TGYEAAEANRPVCVTGAPNKAIAAIAKLLPDDWALALMARQGGRFRKV"
     gene            complement(7766..8464)
                     /locus_tag="PLO65_04305"
     CDS             complement(7766..8464)
                     /locus_tag="PLO65_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRD27505.1"
                     /translation="MTSWEYAVNFFVALFALLDPIGNVPVFAAATAGGIAAGRRWVAA
                     YISLFAFVFMTFFFFTGLSLLEFFGISLPAFRIAGGVILFLMGLEMARDDFTAMFADA
                     AEGVEDAAPRAYARRRFERLIVPFAMPLLIGPGAISTVIIYASGARKLGLEGMATGVG
                     VIAAVSALTMVCFWLTPLISRLLGRIGMTIIVRVLGLILCAMAVQFVIIGVADTARGV
                     IIKKDVSAPYAAEK"
     gene            8523..>8939
                     /locus_tag="PLO65_04310"
     CDS             8523..>8939
                     /locus_tag="PLO65_04310"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRD27506.1"
                     /translation="MSADPRLIVALDLPSRAEAEAMVETLGDAVGFYKIGLQLLAVGG
                     MDMARDLKARGRQIFLDWKLHDIGATVEKAAAAIVGSGACDLLTVHAEPQVLAAAVRG
                     RGADRRAKILGVTVLTSLNGQDLAQIGYGMGVEQLVE"
BASE COUNT         1393 a         3014 c         3095 g         1326 t
ORIGIN      
        1 ggtcgccgcg ttcatgccgg ccaccccgac cagcgcctgg cggcgcccgc gctggatccc
       61 cgcggcgata gcgaacaggt tggcgggacc gggcgtcacg gccatcagcg ccatggcggt
      121 cagaaaggcg acatagcggg cggggtcgac gggcagcagc atggccgggc catatccgcc
      181 ccgccgggca gcgaaaaggg ccgcgcccga gaattcctca tccggctcgc agggaatgcc
      241 gggtgacgga cggcgcgttc cttgtcatgg ctggggcgaa cacatggacg ggatgggcat
      301 gggccccgac ttctggatct tcttcgccgt cggcgtcgcc gcgcagctgg tcgacggcgc
      361 cctgggcatg gcctacggcg tggtctcctc gaccgtgctg ctggcgctcg gcgtgccgcc
      421 ggccaacgcc tcggccagcg tccacgccgc caaggtgttc accggcgcgg cctcggccct
      481 gtcgcacatc gcctatcgca acatcggctg gaagctgctg ctgcatctgt cggccggcgg
      541 ggtgctgggc ggggtgctcg ggacctatgt cctgacttcg atcgacggca aggtgatcaa
      601 gccctgggtg gtcgcctggc tcggcgtcat ggggctggtg attctctggc gcgcctggaa
      661 gggcacgcgg ccgcgggtca tgagctggaa gcggcccggt ccgctgggcc tcgtcggcgg
      721 cttcttcgac gccgtcggcg ggggcggctg ggggccggtg gtcaccagca ccctgatggg
      781 ctcgggggcc gacccccgtc aggccatcgg caccaccaac gccgccgagt tcttcgtcgc
      841 cgtggccatt tcggcgacct tcgtcggcgc catcgtcagc gggcactggg acgccatcgg
      901 cctgcgcgac cacgtctggt ctgtcggggg actgatcgcc ggcggggtgc tggccgcgcc
      961 gctggccggc tggatgacca aggtgctgcc ggtccgcgcc ctgacctggc tcgtcggcct
     1021 gctggtgacc ggcctggcca tctggcaggg cgcgacgctg gccggcctgt cctaggccag
     1081 caggatcagg accgccttgc ccagggcgcc gaacagcagc acgccggcgg ccagggtctg
     1141 gatcaggaag cccagctcac gtttgcccgg atccttgacg tagccgaggg cgtagaggat
     1201 ccggccgacg atccacacca caccgaggcc cgccgcgatc tggcccatcg ggtcctgcgg
     1261 ggcccagaac aggtggaaca gccacaggga cggcaggaag atcggcagcc actccagggt
     1321 gttggcctgg acgcggatgc tgcgctcgag caccggatcg ccggtcatgc agggcgcggc
     1381 gatgccgcac ttgcgccggg cgccggcgac gcccagcccc atccagaagt agaccagcag
     1441 ggccacgagg gtgacgatgg cgacgtaggc ataggactgc attggacgga gcctcccgat
     1501 ttcccgcgac ggtctgcgcc gccgtggcgg aagcctgtca tgcttcgccc ggacccggaa
     1561 cccccggccg cgcaggggct ttagggagcg gggatccagc cgatcgggag gccgccatgc
     1621 gcgtcgaatc cgccgccatc agcgacatcc gctacgacga tgaccgcggc aagctgttcg
     1681 tccggttcgt cgatggtgac gagtacgtct atgtcggcgt tccgggcgag gtccaccgca
     1741 gcttcgtcga ggccgactcg aagggccgct tcttcgccga acagatccgc gaccggtttc
     1801 cctacaaccg cctggacagc taggtcgcag gcctagtcct tcgcggcccg ctggtcgcgc
     1861 ttggccagca cgcgcagccg cagggcgttg agcttgatga agccgccggc gtcgcggtgg
     1921 tcgtaggcca ccttgccctc ctcgaaggtc accaggtcct ggtcgtagag gctgagcggg
     1981 ctggtccggc cgatgaccgt cacgttgccc ttgtagagct tgacccgcac ctggccggag
     2041 acgttcttct ggctgtagtc aatggccgcc tgcagcatct cgcgctcggg cgcgaaccag
     2101 aagccgttgt agaccagcga tgcgtacttc ggcatcagct cgtccttcag gtgcatggcg
     2161 ccgcggtcga gcgtgatcga ctcgatgccg cggtgggcgg ccagcaggat ggtcccgccc
     2221 ggggtctcgt agacgccgcg tgacttcatg ccgacgaagc ggttctcgac caggtcgagc
     2281 cggccgacgc cgttgtcatg gcccagctgg ttgagcgtcg tcagcagggt cgccggcgac
     2341 atggccacgc cgtcgatggc gaccgggtcg cccttgtcga agtcgatggt gaagacgtgc
     2401 ggcttgtccg gcgcgtcctc cggactgatc gtgcgcatgt ggacgaattc gggggcctcg
     2461 accgccggat cctcgagcac cttgccctcg gagctgctgt gcagaaggtt ggcgtcgacg
     2521 ctgaacggcg cctcgccgcg cttgtccttg gtgatctgga tctggtgctt ctcggcgaag
     2581 tcgagcaggg cctcgcggct cttgaagtcc cactcgcgcc agggggcgat gacggtgatg
     2641 tcgggctcca ggccgtagta gccgagctcg aagcggacct ggtcgttgcc cttgccggtg
     2701 gcgccgtggc tgaccgcgtc agcgccggtc ttgcgggcga tctcgatctg cttcttggcg
     2761 atcagcggcc gggcgatcga agtgccgagc aggtactggc cctcatagac ggtgttggcg
     2821 cggaacatcg ggaagacgta gtcgcggacg aactcctcgc gcacgtcctc gatgaagatg
     2881 ttctccggct tgacgcccgc cgccagcgcc ttggcgcgcg ccggctcgat ctcctcgccc
     2941 tggccgaggt cggcggtgaa ggtcaccacc tcggccccgt attcggtctg cagccacttg
     3001 aggatgatcg aggtgtcgag gccacccgaa taggcgagaa cgaccttctt gacggacttg
     3061 tgggcgggca cgggcggtga tcctggggag agacgggttg cggcgcgttc aagggcgccg
     3121 ggggcctccg gtcaaggctt gagttggcct tcggcctcga gcgtcgccag cgcgcgacgc
     3181 agctgggaca gatgcagcac cgcgacgagc ggcgcgagcg tgacccggac gcccagggcg
     3241 atcacggccc cgtcgagcgc cagacccggg gcgcggctct cgaacttcag ccaggcgccg
     3301 gcggcgatgg cgagcgccgg aaccagccag agggccggca gccggtgggc gcgggtcgtg
     3361 gcgacgccgt ccttccacgc cacgggaacc ggcgtcccgg gcccgacccg cttccaggtc
     3421 ccccaggaca gggcgcacat cagggccagg ctcatgatcc agaaggcgtc gccgacgatc
     3481 gagagggcat ggatcatgac cctagaccgt cacctgggcc ccgagctcga ccacgcggcc
     3541 cggcgggatc ttgaagaagt cggtcgggtt ggcggcgttg cgcatcagga agatgaacag
     3601 cttgtcctgc cacagcggca tgccgctgtg ggccgaggcg atcaccgagc ggcggccgag
     3661 gaagaagctg gtggccatga tgtcgaactt cagcccctgc tgcttgcgca gcagcgccag
     3721 cgccttgggc acgttcggtg tttccatgaa gccgtaggtg atgatcagct tcttgaagtc
     3781 gtcgttgacc ggctcgatgc gcacgcggtc ggcctcgctg acgcgcgggg tctcggccgt
     3841 gcgcacggtg gcgatgacgt tcttctcgtg cagcaccttg ttgtgcttga ggttgtgcat
     3901 cagggcgacc ggggtcatgt ccgggtcgct ggtcaggaag atcgccgtgc ccggcgcccg
     3961 atgcggcggc cgcgccttga ggatgtcgat caggtcgttc agcggcaccg agtcgcgacg
     4021 ggtcttgtcg gtgaggatct gggccccctt ggtccaggtc cacatgatca gcaccagcag
     4081 cgccccgaac accagcggca tccaggcgcc ctggggaatc ttcagcaggt tcgaggagat
     4141 gaaggtcagg tccagcgcga cgagcggggc gagcacggcg atcgtcgccg gccaggtcca
     4201 tttccaggcc gccttgatga tcacgaaggc cagcagggtg tcgacgaaca tcgagccggt
     4261 gacggcgatg ccgtaggccg aggccagggc gctggaggtg ccgaagctga acagcagcac
     4321 cagcaccccg atcatgagca tggtgttgac ctgcggcacg aagatctggc cggcctgggt
     4381 ctcggaggtg cggcggatgt cgatgcgcgg cagcaggccc agctgcaccg cctgctgggt
     4441 caccgagaag gcgccggtga tcaccgcctg gctggcgatc accgtggcgc aggccgccag
     4501 cagcaggatc ggccagtagg cgaactgcgg gaccatctcc cagaacggat tatggcgcgt
     4561 ctcgggatgg gcgagaatca gcgagccctg gcccagatag ttgagcgcca ggcaggggaa
     4621 gaccaccacc agccacgagg cgcggatcgg gcctttgccg aagtggccca tgtcggcgta
     4681 gagcgcctcg gccccggtga ccaccaggaa cacgctgccg aggatgacga agcccaggaa
     4741 gccgttctcc cacaggaaac gtaggccgta ccaaggattg aaggcccaga agatcgacag
     4801 gtcttccttg aggtggaaca ggccgagccc gcccagcacc aggaaccaca gcagggtgat
     4861 cggcccgaag aacgacgcca cacggtgggt gccacgcgac tggaccatga acagggcgac
     4921 caggatgcag gccgagatcg gcaggatata gggcgatacc cttgcgccga tgcccggcgc
     4981 gtccttcagg ccctccatcg ccgaaagcac cgagatggcc ggggtgatga tgccgtcgcc
     5041 atagaacagc gccgcgccga cgacgccgag gaagaacacc gccgccgatc gcttgccggt
     5101 ctgcttgctc agggtccggc gggccagcgc catcagggcc agggtcccgc cctcgccctt
     5161 gttgtccgcg cgcatgagca ggatgacgta cttgaccgtc acgatcagga acatcgccca
     5221 gatgaccagc gagacgacgc ccatcaccgc cagctgggtc gcgccgccgc tcttcgaatg
     5281 ggccagggct tcgcgcatgg catagagcgg gctggtgccg atatcgccga agacgacgcc
     5341 gatcgagccg agcgccaggg ccaggaatcc atggccggca tggccgtcct ggccattcgg
     5401 ggtcgtcggg gtggacgccg ccttgtctgg ggcggacgcg ccgggggcct cggaagccat
     5461 cagatccctc cgggatcacg ccgggatagt cccgccgcca agtcccaagc gaatgcggcg
     5521 cgggcgatac acccaatccg ccagctgttc aatgcgtggc cgcggacagg gttcgtcgat
     5581 tttcgtggcg tccggtcccc gcctttccta cctttgccgt cctatcatgt cagacagcca
     5641 gaaatttccc gtcgcggccc acgccctggc ctatctcgcc cacaagcagg cgacgacggc
     5701 ggagcgcgcc atttcgagcg ccgagctggc cgcctcgatg ccgaccaatc cggtcgtcgt
     5761 gcggcgggtg accgccatgc tggcccgggc ggggttgatc gagacgcgcg ccggcgccgg
     5821 cggcggggcc tggctgaagg tcgatccggc cggcatcgcc ctggacaccg tgcttcgggc
     5881 cgtgggcggc tgcacccacc ttggcgtggc cccgccgggc gcaaagggct gcccggtcgg
     5941 agagctgatc cccgaggccg tcagcgcggc catcaccgcc gccgacaccg cggcctccga
     6001 acggctgtcg cggatcaccg tcgccgatct cctcaaggcg cccgcagaaa aactgtgacg
     6061 gaccttgttg ctgatatctt gccgatcagc gggcggttca ttacgtaact gtcactgaga
     6121 cagttcttaa cggggaacca gaatgacccg catcgccatc gcctaccatt ccggctacgg
     6181 ccacaccgaa gccctcgccg ccagcgtcgc ccagggcgtg cgcgacgccg gcgccgaggc
     6241 catcctgctg aggatcgaca acgccggcca ggatttcgac ccgctgctcg acgagatcac
     6301 cgcctcggac gcggtcgtgt tcggcgcccc gacctacatg ggcgacgtgt ccggcgtgtt
     6361 caaagtgttc gccgacgcca cctcgaaggc cttcttcacc ggagcctgga aggacaagct
     6421 ggcggccggc ttcaccaact cgcacagctt ttccggcgac aagctgcatg ccctgaccag
     6481 cctggccatc ctcgcctcgc agcacggcat gaactgggtc aacctgggcg tccccgcgcc
     6541 caacgttccc gccgccgagc gcggacccga gacgctcaac cgcgtgggcg ccttcctcgg
     6601 cctggcgggg cagtcggaca atgcggccgc cgagctcagc ccgccgggcg gcgaccgtga
     6661 aacggcgcga ctgctgggcg tccgggtggc cgaggcggcg cggcgctgga cccagggcgc
     6721 agccagcgaa ccggcgcgca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnncttgaac cgacgcggcg ggcgggccac tctgaggctc agtcttccag
     6901 caggacttcc tgaaaggtcc gtccgcatgc cccgccgcct cgcgctggtg accggcgctt
     6961 ccgccggcat cggcgccgcc ttcgcccgca tctacgccag ccacggctac gatgtggccc
     7021 tgaccgcgcg tcggaccgac cggctcgagg ccctggcggc cgagatccgc ctgcggttcg
     7081 gggtcgaagc cctggtcatc gccgccgacc tcggcgagcc cggcgcctgc gacgccatcc
     7141 tggccgagat cgaatcgcac ggccgggtcg tcgacgccct ggtcaacaat gccggctacg
     7201 gcctggccgg cgcctatgcc gacacccgct ggcctgatca gcggagcatg ctgcaggtga
     7261 tgctggtggc ggtctgcgag ctgacccaca aggtcctgcc cggcatgatc gaccgccggt
     7321 tcgggcggat cgtcaatgtc gcttccctcg ccggactggt gccgggctcg gccggccata
     7381 ccctctacgc ggcgaccaag agcttcctgg tgaagttctc ccaaagcctg cacctggaga
     7441 acctggccac cggcgtgcat gtttcggcgc tctgccccgg cttcaccttt tccgagttcc
     7501 acgacgtcaa cgccacccgc tcccaggtca gccaggcgac gccggaatgg ctgtggctgg
     7561 gggccgacga ggtggcggcc accggttacg aggcggccga ggcgaaccgt ccggtctgcg
     7621 tcaccggcgc gccgaacaag gccatcgccg cgatcgcgaa gctgctgccg gacgactggg
     7681 ccctggcgct gatggcgcgg cagggcgggc ggttccggaa ggtctgaggc tcgggccgcc
     7741 gatcactctc gcggcagggc ccgtcctact tctccgccgc gtagggcgcg gagacgtcct
     7801 tcttgatgat caccccgcgc gccgtgtcgg ccacgccgat gatcacgaac tgcacggcca
     7861 tggcgcagag gatcaggccc agcacccgca cgatgatggt catgccgatc cggcccagca
     7921 ggcgcgagat cagcggggtg agccagaagc agaccatggt cagcgccgac accgcggcga
     7981 tcacgcccac gccggtggcc atgccctcca ggcccagctt cctcgccccg ctggcgtaga
     8041 tgatcaccgt cgagatggcc ccgggcccga tcagcagggg catggcgaac ggcacgatca
     8101 gccgctcgaa ccggcgccgc gcgtaggcgc gcggagccgc gtcctcgacc ccctcggccg
     8161 cgtcggcgaa catggcggtg aagtcgtcgc gcgccatctc caggcccatc aggaacagga
     8221 tcaccccgcc ggcgatgcgg aaggccggca gcgagatgcc gaagaactcc agcagcgaca
     8281 ggccggtgaa gaagaagaag gtcatgaaga caaaggcgaa cagggaaatg taggccgcca
     8341 cccagcggcg gcctgccgcg atcccgcccg ccgtcgccgc ggcgaacacc ggcacgttgc
     8401 cgatcgggtc gagcagcgcg aacagggcga cgaagaagtt gacggcgtat tcccaggagg
     8461 tcatgcagct tccttagccc aaatcacgcc agcgcgcctc cccgagaggc gggagccgcc
     8521 gcatgtccgc cgacccgcgc ctgatcgtcg ccctcgacct ccccagccgc gccgaagcgg
     8581 aggcaatggt cgagaccctg ggcgacgcgg tcggtttcta caagatcggc ctgcagctgc
     8641 tggccgttgg cggcatggac atggcccgcg acctcaaggc ccggggccgc cagatcttcc
     8701 tggactggaa gctgcacgac atcggcgcga cggtggagaa ggcggcggcg gcgatcgtcg
     8761 gctccggcgc ctgcgacctg ctgaccgtgc atgccgagcc gcaggtgctg gccgcggcgg
     8821 tgcggggccg cggcgcggat cgccgggcga agattctcgg cgtcaccgtg ctgaccagcc
     8881 tcaacggcca ggacctggcg cagatcggct acggcatggg cgtcgagcag ctggtcgag
//
LOCUS       DAORNX010000063         8897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5820_length_8897_cov_7.086745, whole genome shotgun sequence.
ACCESSION   DAORNX010000063 DAORNX010000000
VERSION     DAORNX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5820_length_8897_cov_7.086745"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLO65_04315"
     CDS             complement(<1..180)
                     /locus_tag="PLO65_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27507.1"
                     /translation="MAARAAHSLALVRAEIAEQARRWTLWAPVAFGLGAAGYLALREE
                     PPLWLAAPLAALAVAV"
     gene            218..1630
                     /gene="gltX"
                     /locus_tag="PLO65_04320"
     CDS             218..1630
                     /gene="gltX"
                     /locus_tag="PLO65_04320"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRD27508.1"
                     /translation="MSDSRPVVTRIAPSPTGSMHIGTARTALFNYLYAKHTGGRFLLR
                     VEDTDRERSTEAAVQVIFDGLAWLGLPFDGEPVFQHARADRHRAVVLEMLAAGKAYRC
                     WLSGDELVAAREKAHAAGHALRSPWRDRDPGPTDLARPHAIRFRGPLNGDTVVEDLVQ
                     GQVTFRNRDLDDLVLLRSDGAPTYNLAVVVDDHDMDVTHIIRGDDHLNNAARQSLLYQ
                     AMGWQAPAFAHIPLIHGEDGAKLSKRHGATAVTDFAALGYLPEAMRNYLVRLGWSHGD
                     DELFSDEQAVAWFDIVDANKGASRLDWDKLNFVNAHYIRLADDARLLALVAEVYGARG
                     DPLDDVRRAHALRAIPLVKERARTIVELADQLAFLYRPRPLKLDDKALGLLTDETRAR
                     LLRLRAGLAAANDWGAEALEALLKAFAGSEGVGFGKFAPALRAILTAGMASPDLGRTL
                     SALGREESLARIDDVLSPAA"
     gene            1690..2970
                     /gene="gltA"
                     /locus_tag="PLO65_04325"
     CDS             1690..2970
                     /gene="gltA"
                     /locus_tag="PLO65_04325"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRD27509.1"
                     /translation="MTDKAMLTIGDKSFELPILKGSTGPDVVDVRKWYADSDTFTFDP
                     GFTSTASCESKITFIDGDAGILLHRGYPIDQLAEKSSFLEVCYLLLHGELPTADEFSK
                     FDRNITYHTMLNAQFDRFFEGFRRDAHPMAVMTGAVGALSAFYADSMNVDDAREREIS
                     AHRLIAKMPTIAARAFKYTIGQPFVSPRNELSYSENFLRMCFAVPAEDWKPNPVLTRA
                     MDRIFILHADHEQNASTSTVRLAGSSGAHPFACIAAGIACLWGPSHGGANQEALEMLE
                     TIGTVDRIPEFIQGVKDREYKLMGFGHRVYKNFDPRAKVMQKTCHEVLKELGINDPLL
                     EVAMELEKIALSDPYFIERKLYPNIDFYSGITLRAMGFPTNMFTVLFALARTVGWISQ
                     WKEMFEDPQRKIGRPRQLYTGPTQRDYAPIDKRG"
     gene            3034..3339
                     /locus_tag="PLO65_04330"
     CDS             3034..3339
                     /locus_tag="PLO65_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HRD27510.1"
                     /translation="MAKPLTVSIPHQLGVAEARRRLETGLAEMTAKLPGGAAKIDQHW
                     DGDRLLFDAQVSGQSITGSLHVLADAVRMEIVLPGILGLLAGKIGGRLKDEGQKLLK"
     gene            complement(3424..4605)
                     /locus_tag="PLO65_04335"
     CDS             complement(3424..4605)
                     /locus_tag="PLO65_04335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD27511.1"
                     /translation="MQTFDVVIVGAGAAGMMCAIEAGRRGRSVLVIDHARAPGEKIRI
                     SGGGRCNFTNLNTGPQAFLSQNPRFCLSALRRYTPKDFIAAVDARGIAWHEKTLGQLF
                     CDGSAMQIVTMLTDDMAQAGVDLRLGLDVERVDREGEGFALTLSDGARVGCASLVVAT
                     GGKSIPKMGATGWAYDIARQFGLNIIETRPALVPLTFEIGLLQRWKDLSGVAVDALVA
                     SGKTTFAEAMLFTHRGLSGPSILQISSYWREGQEIAVSMAPGVDVFAALKAAKGSNPR
                     QAVHTALGAFLPDRLARKLAEEAGARGNLADTPDAVLRALDAAVNHWRVKPVGSEGYR
                     TAEVTLGGVDTRDLNSTTMAANAVPGLYFIGECVDVTGWLGGYNFQWAWSSGWAAGQV
                     A"
     gene            complement(4608..4820)
                     /locus_tag="PLO65_04340"
     CDS             complement(4608..4820)
                     /locus_tag="PLO65_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27512.1"
                     /translation="MQRPACVIAYRDSGQVCTDSDQCDGRCVADDGYSPKAPGPVAAH
                     CEADSDRCGCATEVLGGVAQATLCID"
     gene            complement(5095..6270)
                     /gene="lpxB"
                     /locus_tag="PLO65_04345"
     CDS             complement(5095..6270)
                     /gene="lpxB"
                     /locus_tag="PLO65_04345"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRD27513.1"
                     /translation="MTRPLTVMLVAAEASGDALGAGLAHALKARLGDRVRLVGVGGRR
                     MAAEGIDSPFDIASLSILGIFEAVAAYRTVVRRVADTVALAQRETPDLAVLIDSWGFT
                     LRVAQALRRTFPGMPLVKYVGPQVWATRPGRAKTLAASVDHLLSIHSFDAPWFEKEGL
                     KTTFVGNPALAKDFSHADPARLRTAIGAGQDDDILLVLPGSRPAEIKLVMPVFEAAVK
                     RLKADRPGLHIVIPAAGTVIDSVRAKVAGWPFRAHVVEDEQLKDDAFVAGTVALACSG
                     TVTTELALAGTPMVVAYRIGAATYAILKLIFKSPWVTMFNIAAKAFVAPEFLQAECTP
                     DNLARAIADRLDDPALRARQVDAQNAALELMGRGGPDPSEKAADTIIALLAERGVKV"
     gene            complement(6267..7106)
                     /gene="lpxI"
                     /locus_tag="PLO65_04350"
     CDS             complement(6267..7106)
                     /gene="lpxI"
                     /locus_tag="PLO65_04350"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079315.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRD27514.1"
                     /translation="MRKLGIIAGGGGLPAEIADRCRRTGRPYFVIRLRGFADEGTTGH
                     PSADLGLGELGKVFKVLHQEHCEAVCLAGTVARPDFSKLKPDLRGMAALPSIVAAARK
                     GDDGLLRAVLDEFRKEGFAIEGAHEAARSMTLPLGCLGAVAPGDRHAEDIQRALIAAR
                     AIGRLDIGQGAVVCDGLVLAVEAQEGTDAMLRRVRELPEAIRGAPGGAKGVLAKAPKP
                     IQSTDVDLPTIGVATVRAAAQAGLAGIVGEAGRLLVVDREETAAMADDLGLFILGVEA
                     KDE"
     gene            complement(7109..7903)
                     /gene="lpxA"
                     /locus_tag="PLO65_04355"
     CDS             complement(7109..7903)
                     /gene="lpxA"
                     /locus_tag="PLO65_04355"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRD27515.1"
                     /translation="MTKIHPTAIVDKAADLDPSVEIGPYCLVGPGARLGKGVRLLSHA
                     IVEGVTEVGEDCVLHPFACLGGPPQHSAHRGEPTRVVVGARNVFREHVTVHAGTAGGR
                     GVTTIGSDGLFMVSVHVGHDCIIGDKVIMAPSVAVGGHVTVNDFVFLGGLAAIHQFTR
                     IGRYAFVGGGGIVTKDVIPYGSVWGNHAHLEGLNLIGLKRRNFTREQIATLRAAYRLL
                     FADEGTFQERLDDVTETYPDVPEIMEIVAFIRDDATRPLCLPEREV"
     gene            complement(7900..8367)
                     /gene="fabZ"
                     /locus_tag="PLO65_04360"
     CDS             complement(7900..8367)
                     /gene="fabZ"
                     /locus_tag="PLO65_04360"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRD27516.1"
                     /translation="MTDGETALPTIEITEVLARLPHRYPFLLVDRAEDYKAGESLVGI
                     KCVTVNEPFFPGHFPGNPVMPGVLVVEALAQVGALLMSKTWDVDMEKSTIFFMSVDGA
                     RFRLPVRPGDVLKLHVEVLRIRAPVVKFSGKAMVNGRLAAECEFAAMKADNPQ"
     gene            complement(8360..>8897)
                     /locus_tag="PLO65_04365"
     CDS             complement(8360..>8897)
                     /locus_tag="PLO65_04365"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRD27517.1"
                     /translation="IGPNASIGFALLGDRVRIYAGARIGEAGFGATAGGEGVVDVPQL
                     GRVILQDGVTIGANGCIDRGAWDDTVIGENTKLDNQVHIAHNVRVGRNCVMAAYSGVS
                     GSVTIGDGCAFGGRTGIADHVTIGDGVQIAAGAGVFRDVPSGETWGGIPAQPIRNWLR
                     ETALLSRMAREKKRTGHD"
BASE COUNT         1419 a         3183 c         2987 g         1308 t
ORIGIN      
        1 gaccgcaacg gcgagggccg ccaggggcgc ggccagccac agcggcggtt cctcgcgcag
       61 ggccagatag ccggcggcgc ccaggccgaa cgccaccggg gcccagaggg tccagcgccg
      121 ggcctgctcg gcgatttcag cgcgaacgag cgcaaggcta tgtgcggcgc gggcggccat
      181 gctaagaggc ccgcccagcc gcgcagaccc cacagagatg tccgactcca ggcccgtcgt
      241 cacccgaatc gccccctcgc cgaccggctc gatgcacatc ggcacggcgc gcaccgccct
      301 gttcaactac ctatacgcga aacatacggg cgggcgcttt cttttgcgcg tggaggatac
      361 cgatcgcgaa cggtcgaccg aggccgcggt gcaggtgatc ttcgacggcc tggcctggct
      421 gggcctgcca ttcgacggcg agccggtgtt ccagcacgcc cgcgcggatc ggcaccgggc
      481 cgtggttctg gagatgctcg cggccggcaa ggcctatcgc tgctggctgt ccggagacga
      541 actggttgcg gcccgggaga aggcccacgc ggccggccac gccctgcggt cgccctggcg
      601 cgatcgcgac cccggtccga ccgatctcgc caggccgcac gcgattcgtt tccgcggccc
      661 gctgaacggc gacaccgtgg tcgaggacct cgtccagggc caggtgacct tccgcaaccg
      721 cgacctggac gaccttgtcc tgctgcgctc ggacggcgcc ccgacctaca acctcgccgt
      781 ggtagtcgac gaccacgaca tggacgtcac ccacatcatt cgcggcgacg accacctgaa
      841 caacgccgcc cggcagagcc tcctgtacca ggcgatgggc tggcaggcgc cggccttcgc
      901 ccacatcccg ctgatccacg gcgaggacgg ggccaagctc agcaagcgcc acggggccac
      961 ggcggtgacc gatttcgccg cactgggcta cctccccgaa gcgatgcgga actacctcgt
     1021 ccgcctgggc tggagtcatg gcgacgacga gctgttcagc gacgagcagg ccgtcgcctg
     1081 gttcgacatc gtcgacgcca acaagggcgc ctcgcggctc gactgggaca agctcaactt
     1141 cgtcaacgcc cactacatcc gtctggccga cgacgcccgc cttctggcgc tggtcgctga
     1201 ggtctatggc gcccgtggcg atccgctgga cgacgtgcgc agggcgcatg ccctgcgcgc
     1261 catcccgctg gtcaaggagc gggccaggac catcgtcgag ctggccgacc agctggcctt
     1321 cctctaccgg cccaggcccc tgaaactcga cgacaaggcc ctgggcctgc tcaccgacga
     1381 gacccgcgcg cgccttctgc ggctgcgcgc cggcctggcc gccgccaacg actggggcgc
     1441 cgaggcgctt gaggcgttgc tcaaggcgtt cgccgggtcg gaaggcgtcg gcttcggcaa
     1501 gttcgcgcct gccctgcgcg cgattctgac cgccggcatg gcatcgccgg accttggacg
     1561 cacgctttcg gccctgggcc gcgaggagag tctggcgcgc atcgacgacg tgctttcgcc
     1621 tgccgcgtga accgctataa cggcgccgaa cgcccgttcg ggcgaccatg atttcgaggg
     1681 gattgaagca tgaccgacaa ggcgatgctg acgatcggcg acaagagctt cgagctgccg
     1741 atcctcaagg gctccaccgg cccggatgtg gtcgatgttc gcaaatggta cgccgactcc
     1801 gacaccttca ccttcgatcc gggcttcacc tcgacggcgt cctgcgagag caagatcacc
     1861 ttcatcgacg gtgacgcggg gatcctgctg caccgcggct acccgatcga ccagctggcc
     1921 gagaagtcca gcttcctcga ggtctgctac ctgctgctgc atggggaact gccgacggcc
     1981 gacgagttca gcaagttcga ccgcaacatc acctaccaca ccatgctgaa cgcccagttc
     2041 gatcgcttct tcgagggctt ccgccgcgac gcccatccga tggcggtgat gaccggcgcg
     2101 gtcggcgccc tgtcggcctt ctacgccgac agcatgaacg tcgacgacgc ccgcgagcgc
     2161 gagatcagcg cccatcgcct gatcgccaag atgccgacga tcgccgcgcg ggcgttcaaa
     2221 tacacgatcg gtcagccgtt cgtgtcgccg cgcaacgagc tcagctactc cgagaacttc
     2281 ctgcgcatgt gcttcgccgt gccggccgag gactggaagc ccaatccggt gctgacccgc
     2341 gcgatggacc gcatcttcat cctgcacgcc gaccacgagc agaacgcctc gacctcgacg
     2401 gtgcggctgg ccggttcgtc cggcgcccat ccgttcgcct gtatcgccgc cggcatcgcc
     2461 tgcctgtggg gcccctcgca cgggggcgcc aaccaggagg ccctggagat gctcgagacc
     2521 atcggcacgg tcgacaggat tcccgagttc atccagggcg tgaaggaccg cgagtacaag
     2581 ctgatgggct tcggccaccg cgtgtacaag aacttcgacc cccgggcgaa ggtcatgcag
     2641 aagacctgcc acgaggtgct caaggagctg ggcatcaacg acccgctgct cgaggtggcg
     2701 atggagctgg agaagatcgc cctcagcgat ccctacttca tcgagcgcaa gctctatccg
     2761 aacattgact tctattccgg catcaccctg cgggcgatgg gcttcccgac caacatgttc
     2821 accgtgctgt tcgccctggc ccgcaccgtc ggctggatca gccagtggaa ggagatgttc
     2881 gaggatcccc agcgcaagat cggccggccg cgccagctct acacgggccc cacccagcgg
     2941 gactacgcgc cgatcgacaa gcgcggctga cggcgccgcg tacggatttc cccaggcctc
     3001 ggagcaatcc ggggcctgtt tcgttggaac atcatggcca agcccctcac cgtctccatt
     3061 ccccaccaac tcggcgtggc cgaggcccgc cgacggctcg aaaccggcct ggcggaaatg
     3121 accgccaaac tgccgggcgg cgccgcgaag atcgaccagc attgggacgg cgaccggctg
     3181 ctgttcgacg cccaggtcag cggccagagc atcaccggct cgctgcatgt gctggccgac
     3241 gcggtgcgga tggagatcgt cctgcccggc atcctgggcc tgctggccgg caagatcggc
     3301 ggccggctga aggacgaagg gcagaagctg ttgaagtaag caggggacat gtaccgcagg
     3361 tacgtgtccc ctaatcgcgc cgcgaacctg gattggggga cacgtacctt tggtacatgt
     3421 cccctacgcc acctgccccg ccgcccagcc gctcgaccag gcccactgga aattgtaacc
     3481 gccgagccag ccggtcacgt cgacgcattc gccgatgaaa tagagcccgg gcacggcgtt
     3541 ggcggccatg gtcgtcgagt tcaggtcgcg cgtgtcgacc ccgcccaggg tgacttcggc
     3601 cgtgcggtag ccttccgacc ccaccggctt gacccgccag tggttgaccg ccgcatccag
     3661 cgcccgcagc acggcgtcgg gcgtgtcggc caggttgccg cgcgcgccgg cctcctcggc
     3721 cagcttccgg gcaagccggt cgggcaggaa ggcccccagg gcggtatgca ccgcctggcg
     3781 cgggttcgag cccttggcgg ccttcagcgc cgcgaacacg tccacgcccg gcgccatcga
     3841 tacggcgatc tcctggccct cgcgccaata ggacgagatc tgcaggatcg acggcccgct
     3901 gaggccgcga tgggtgaaca gcatggcctc ggcgaaggtc gtcttgccgc tggccaccag
     3961 cgcgtcgacc gcgacgccgg acaggtcctt ccagcgctgc agcaggccga tctcgaaggt
     4021 cagcggcacg agggccggcc gcgtctcgat gatgttcagg ccgaactggc gggcgatgtc
     4081 gtaggcccag cccgtcgcgc ccatcttcgg gatcgacttg ccgcccgtgg ccaccaccag
     4141 cgacgcgcag ccgacccggg cgccgtccga cagggtcagg gcgaacccct ccccttcgcg
     4201 gtcgacgcgt tcaacgtcca ggcccaggcg caggtccacg ccggcctgcg ccatgtcgtc
     4261 ggtcagcatg gtcacgatct gcatcgccga gccgtcgcag aacagctggc ccagcgtctt
     4321 ctcgtgccag gcgatgccgc gcgcatcgac ggccgcgatg aagtccttcg gggtgtagcg
     4381 ccgcagcgcc gacaggcaga agcgcggatt ctgcgacagg aaggcctgcg gaccggtgtt
     4441 gaggttggtg aagttgcaac gaccgccacc ggagatgcga atcttctcgc ccggcgccct
     4501 ggcgtgatcg atgaccagca ccgagcggcc gcggcgaccc gcctcgatgg cgcacatcat
     4561 gccggccgcc ccggccccga cgatgacaac atcaaaagtc tgcatgatca gtcgatgcag
     4621 agggtggctt gcgccacgcc gccgagaacc tcggtggcgc agccgcagcg gtcgctgtcg
     4681 gcttcgcaat gggcggcgac cggacccggg gccttggggc tatagccgtc gtcggccaca
     4741 cagcggccgt cgcactggtc gctgtcggtg cagacctggc cggagtccct gtaggcgatc
     4801 acgcaggcag gacgttgcat gcggcaaacc ggccggatcg caccgccggc ggctctgcag
     4861 gcggcggggt cggcggccag ggcgacttgc gtcgtcggcg gcggcgcagg cgcagggccg
     4921 gcgacagggg tgcaggcggc cacggcgagg accaggacag cgaggagaac ggggcgcatg
     4981 ggctggttct acgccgcccc cggccgttcg gcgagcctgc ggaaggccgc cgtcctccat
     5041 cgaggagcat ggggagccac cgcgaaggtt tcagcccccc ggattcgtcg gccctcagac
     5101 cttcacgccc cgttcggcga gcagggcgat gatcgtgtcc gccgccttct ccgacgggtc
     5161 gggcccgccc ctgcccatca gctccagcgc cgcgttctgg gcgtcgacct ggcgggcccg
     5221 cagagcgggg tcgtccagtc ggtccgcgat ggcccgggcc agattgtccg gcgtgcattc
     5281 cgcctgcagg aactccggcg cgacgaaggc cttggccgcg atgttgaaca tcgtcaccca
     5341 gggcgacttg aagatcagct tgagaatggc gtaggtcgcc gcgccgatcc tgtaggccac
     5401 caccatcggc gtgccggcca gggccagctc ggtggtcacc gtgccgctgc aggccagggc
     5461 gaccgtaccg gcgacgaagg cgtcatcctt gagctgctcg tcctcgacca catgggcgcg
     5521 gaatggccag ccggcgacct tcgcccgcac cgagtcaatc acggttccgg cggcggggat
     5581 gacgatgtgc aggcccggtc ggtcggcctt gagccgtttc accgccgcct cgaacaccgg
     5641 catgaccagc ttgatctcgg ccgggcggct gccaggcaga accagcagga tgtcgtcgtc
     5701 ctgccccgcc ccgatcgcgg tgcgcagacg ggccggatcg gcgtgcgaga agtccttcgc
     5761 cagggccgga ttgccgacga aggtggtctt cagcccctcc ttctcgaacc agggggcgtc
     5821 gaagctgtgg atcgacagca gatgatcgac gctggccgcc agggttttcg cccgccccgg
     5881 ccgcgtggcc cagacctgcg gcccgacgta cttgaccagc ggcatgcccg gaaaggtcct
     5941 gcgcagggcc tgggcgacgc gaagggtaaa gccccagctg tcgatcagca cggccaggtc
     6001 cggcgtctcg cgttgcgcca gggccacggt gtcggccacg cgccggacca ctgtccggta
     6061 ggccgcgacg gcctcgaaga tgccgaggat cgacaggctg gcgatgtcga acgggctgtc
     6121 gatcccctcg gcggccatcc ggcggccacc gacgcccacc agccgcaccc ggtcgccgag
     6181 ccgggccttg agggcgtggg cgagaccggc gccgagcgcg tcacccgagg cctcggcggc
     6241 caccagcatg acggtcaggg gccgggtcat tcgtccttcg cctcgacccc caggatgaac
     6301 aggcccaggt cgtcggccat ggccgcggtc tcctcgcggt cgaccaccag cagccggcct
     6361 gcctcgccga cgattcccgc cagcccggcc tgggcggcgg cccgaaccgt cgcgacgccg
     6421 atggtcggca ggtcgacatc ggtggactgg atcggcttgg gcgccttggc cagcacgccc
     6481 ttggcgcccc cgggcgcgcc gcggatggct tcaggcaact cgcgcacacg acgcagcatg
     6541 gcgtccgtgc cttcctgagc ctcgaccgcc agcacaaggc cgtcgcagac caccgccccc
     6601 tggccgatgt cgagccgccc gatcgcccgg gcggcgatca gcgcccgctg gatgtcctcg
     6661 gcgtggcggt ctccgggcgc caccgcgccc aggcagccga gcggaagggt catcgagcgg
     6721 gccgcctcat gggccccctc gatggcgaag ccctccttgc ggaactcgtc gagcaccgcc
     6781 cgcagcaggc cgtcgtcgcc cttgcgagcc gcggcgacga tggacggcag ggccgccatg
     6841 ccgcgcagat ccggcttgag cttgctgaaa tcgggccggg ccaccgtgcc agccaggcaa
     6901 acggcctcgc agtgctcctg atggagcacc ttgaacacct tgccgagctc gcccaggccc
     6961 aggtcggcgg acggatgacc ggtcgtgccc tcgtcggcga aaccgcgcag gcggatcacg
     7021 aagtacggcc ggcccgtgcg ccggcagcga tcagcgattt ccgccgggag ccccccgccc
     7081 ccggcgatga tcccgagctt gcgcacgctc agacctcgcg ctcgggcagg cacagcgggc
     7141 gggtcgcgtc gtcgcggatg aaggcgacga tttccatgat ctcggggacg tccgggtagg
     7201 tctcggtgac gtcgtccagt cgttcctgga aggtgccctc gtcggcgaac agcaggcggt
     7261 aggcggcgcg cagggtggcg atctgctcgc gagtgaaatt gcgccgcttc agaccgatca
     7321 ggttgagccc ctcgaggtgg gcgtggttgc cccagaccga gccgtagggg atcacgtcct
     7381 tggtgacgat gccgccgccg ccgacgaagg catagcggcc gatgcgggtg aactggtgga
     7441 tcgccgccag cccgccgagg aagacgaagt cattgactgt cacatggccg cccacggcca
     7501 cgctgggggc catgatgacc ttgtcgccga tgatgcagtc gtggccgaca tggacgctga
     7561 ccatgaacag gccgtccgag ccaatcgtgg tcacgccgcg gccgcccgcc gtgccggcgt
     7621 gaacggtgac gtgctcgcgg aacacgttgc gggcgccgac caccacccga gtcggttcgc
     7681 cccggtgggc cgagtgctgc ggcggcccgc ccaggcaggc gaacggatgc agcacgcaat
     7741 cctcgccaac ctcggtgaca ccctcgacga tggcatgcga cagcagcctg acgcccttgc
     7801 ccagcctggc gcccggcccg accaggcaat agggcccgat ctcgacgctc gggtcgagat
     7861 cggcggcctt gtcgacgatg gccgtggggt gaatcttcgt cactggggat tgtccgcctt
     7921 catcgccgcg aattcgcatt cggccgccag cctgccgttg accatcgcct tgccggaaaa
     7981 cttcacgacc ggcgcgcgga tgcgcaaaac ctcgacatgc agcttcagca cgtcgccggg
     8041 ccggaccggc agacggaacc gcgcgccatc aaccgacatg aagaagatcg tcgacttctc
     8101 catgtcgacg tcccaggtct tggacatcag cagggcgcca acctgggcca gggcctcgac
     8161 caccagcacg ccgggcatca ccggattgcc cgggaaatgt ccgggaaaga agggctcatt
     8221 gacggtcacg cacttgatgc cgaccagcga ttccccggcc ttgtagtcct cggcccggtc
     8281 gaccagcagg aacggatagc ggtgcggcag tcgcgccagc acttcggtga tctcaatggt
     8341 cggcagcgcg gtttcgccgt cagtcatggc ccgtcctctt cttttcgcgc gccatgcgcg
     8401 acagcagggc cgtctcgcgc agccagttgc ggatcggctg ggcgggaatg ccgccccagg
     8461 tctcgccgga cggcacatca cggaacaccc cggccccggc cgcgatctgc accccgtcgc
     8521 cgatggtcac gtggtcggcg atgccggtcc gtccgccaaa ggcgcaaccg tcgccgatcg
     8581 tcacgctgcc cgagactccc gaataggcgg ccatgacgca gttccgccca acccgcacgt
     8641 tgtgggcgat gtgcacctgg ttgtcgagtt tggtgttctc gccgatcacc gtgtcgtccc
     8701 aggcgccgcg atcaatgcag ccgttggcgc cgatggtcac gccgtcctgg agaatcaccc
     8761 gccccagctg cggcacgtcg acgacgcctt cgccgcccgc cgtcgctccg aagccggcct
     8821 cgccgatgcg ggcgccggcg tagatgcgca cccggtcgcc gagcagcgcg aagccgatcg
     8881 aggcgttggg gccaatc
//
LOCUS       DAORNX010000064         8773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5924_length_8773_cov_6.745699, whole genome shotgun sequence.
ACCESSION   DAORNX010000064 DAORNX010000000
VERSION     DAORNX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_8773_cov_6.745699"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PLO65_04370"
     CDS             <1..1606
                     /locus_tag="PLO65_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRD27518.1"
                     /translation="GLTILEVTDGAGRKLAWTLGAAEGDKGAPLTVQLAGATRVVVAY
                     RSAPGALALQWLPPELTAGKVKPFLYSQGQPTLNRSWIPTQDSPGVRQTWTARIVVPE
                     GLTAVMSGEKLTPGGEPAGPGRRAFRFRMTHPTAPYLIALAAGDIAFQALGPRTGVYT
                     EPAMMARAAAELVDTEKMVEAAEALYGPYRWGRYDVLVLPPAFPYGGMENTTLTFLTP
                     TFITGDRANVGLVAHELAHSWSGNLVTNATWDDIWLNEGFTSYVENRMMEALYGPERA
                     GIEADLSWDELQRAVASRPAAEVTLFGAGTGVEYIKGEAFLRTLEHAFGRDRWDAYLR
                     GYFDRFAFQPQTTAGFLADLREHLVKGDAALEAKLQLDEWVYRPGIPSGAWHEASPAL
                     LTVDAARTAFLGGGPAAAIPGKGWTTQEWKRFLDGLPRRLTPAQLAGLDDAFGLSDST
                     NAYVRSAWLELVVANRYAPALPSLQTFLASNGRGLFLNPLYNGLLKDKAWGLPLARRY
                     FEAAKSGYHPALAASIEKRIAAAEMP"
     gene            complement(1625..2041)
                     /locus_tag="PLO65_04375"
     CDS             complement(1625..2041)
                     /locus_tag="PLO65_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27519.1"
                     /translation="MTPFVFDTLSASKQLREAGMPEGVAEAVVSVFQHASAMPDISHL
                     ASKADIADMATRADLELLRVATKADIESLRQSTSADTHVLRTEMATKAELAQLRLEIA
                     SFRAAITDTIRQQGWLLLGGIGVLMGMFTFIGRTLG"
     gene            complement(2108..2359)
                     /locus_tag="PLO65_04380"
     CDS             complement(2108..2359)
                     /locus_tag="PLO65_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HRD27520.1"
                     /translation="MPAVGLEKDDRAALARALRGYLKDELDVEIGGLEAEFLLDFMGE
                     RLGPRFYNRGLRDAATALAKRMDVLTEALEELERPVPRD"
     gene            complement(2359..3294)
                     /gene="argF"
                     /locus_tag="PLO65_04385"
     CDS             complement(2359..3294)
                     /gene="argF"
                     /locus_tag="PLO65_04385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRD27521.1"
                     /translation="MSSPRHFLDIWKLEAHDLRVILDDARARKAARRGWTQARVDADA
                     PAEGRTLAMIFEKNSTRTRFSFDAAIRQLGGSGIISTAADMQLGRGEPIEDTARVLSR
                     MVDAVMIRANRHEDVELFAQNATIPVINGLTDKSHPCQILADILTFEEHRGPIAGKTI
                     AWVGDGNNVCSSFIHAAPKLGFKLNIACPAAYHPDLYDLARAAEQQGRIEMTEDPREA
                     VRGADCVVTDTWVSMGDVDGEARLDAFESYQVDEDLMALADRKAVFLHCLPAHRGEEV
                     SDAVIDGPQSLVWDEAENRIHAQKSILAWCFGKIG"
     gene            complement(3418..4641)
                     /locus_tag="PLO65_04390"
     CDS             complement(3418..4641)
                     /locus_tag="PLO65_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD27522.1"
                     /translation="MTTPTAPTATPDHIMGVYNRAPLAVDRGLGARLWDVNGVEYLDC
                     VAGIATNGLGHCHPKLVETLKVQADKLWHVSNIFRIPGQEALARALTDATFADVVFFT
                     NSGTEAIECALKTARKYHTANGHPERVDIIGFDGSFHGRSYAAITAAGNPGYVEGFGP
                     RLPGYVQAKWGEMDALKALIEQPTTAAVILEPVQGEGGARAMPEADLDQIRAWTRENG
                     VLLIYDEVQSGMGRTGKLFAHEWASDAAPDIMAVAKALGGGFPVGACLATAEAAKGMT
                     VGVHGSTFGGNPLAMAVGLAAFDEISKDETLANVRDVAGYFNQQLTGLKERFPDIIVD
                     VRGKGFLIGLKVTPVNREFMAMARDQKLLVAGGGDNCVRLLPSLLLTVEEAREAVEKL
                     EKTCEAARAKAAAAA"
     gene            4874..5224
                     /locus_tag="PLO65_04395"
     CDS             4874..5224
                     /locus_tag="PLO65_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HRD27523.1"
                     /translation="METTERTRNLAKRLRRRMNEPELRLWSRVRKNQLDGIHIRRQHP
                     LGHYVLDFFCARSRLAIEVDGGMHDEDRVARDEVRDAWLAMRGVRTLRLPASLVMNDM
                     EAALAAIRRRLTTL"
     gene            complement(5325..5801)
                     /locus_tag="PLO65_04400"
     CDS             complement(5325..5801)
                     /locus_tag="PLO65_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27524.1"
                     /translation="MIAHFSIPARDPQATAELFARIIDGVAVRFPVVDGGWMVLARDG
                     SGTGVEVIPDTAVIAAGEGPIDGARFTSGPREAPAGPTAIHFALTSALSTDEVIALGE
                     AEGWRAVHADRGPFDLVELWIDNRIMVEVLPPAGAARYMASVRSQLAALAATPEFA"
     gene            6069..6344
                     /locus_tag="PLO65_04405"
     CDS             6069..6344
                     /locus_tag="PLO65_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27525.1"
                     /translation="MSDAVGPGDLVLCVNAAPNHVTGQPVPLVAGETYRVWTVMDFAC
                     PCGRSDALLDIGIDFAWCQSRFRLLPKPKAQSKPVKVSAPKEKERVR"
     gene            complement(6444..7325)
                     /locus_tag="PLO65_04410"
     CDS             complement(6444..7325)
                     /locus_tag="PLO65_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRD27526.1"
                     /translation="MKQAVIAALATVLSVGSAASSPPPTATAEWVAGFAQPTPRARLD
                     HLTAMLADLGLEHEVRPFTLKRAAGEGHNVIITLGEGDRDILLTAHYDARRLKDGRLA
                     DGVVDNAASVVALARAAVLLRGGLQNHRLRIIFFDQEELGLLGAKAYAASDDGRRAAA
                     IFNFDINAYGDTPYYALPGDAENALLKGVIEDGCRAANAPCRPFEVYPSSDHRAFWLR
                     HIPATSFSYLPRAEVEALDAFMRRGRGGWLNPLPVPAVLRLIHSPNDRITAVDPATVD
                     RTTRLAVAMARAADARR"
     gene            complement(7342..8163)
                     /locus_tag="PLO65_04415"
     CDS             complement(7342..8163)
                     /locus_tag="PLO65_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD27527.1"
                     /translation="MKDMAESVLPPQPRDYAGFNWTGFSTLYLKEVRRFWKVGTQTIA
                     APVVTTLLYMMVFVVAVRGAAPPIHGTPFSAFVAPGLIMMAMINNAFANSSSSLLQAK
                     MMGLTPDFLTPPLSALEQVLAFTLGAATRGVIVGAVTALTIAILPFTQLQVAHPWAVI
                     YFAVGASLLMGLVGIATGLWSEKFDHLAAITNFLIMPMTFLSGTFYLVDKLPEPFRTA
                     SHFNPFFYLIDGFRYGFIGEADGDLTVGVVLTGVLAVALFLWSWRVFVTGWRLKT"
     gene            8594..>8773
                     /locus_tag="PLO65_04420"
     CDS             8594..>8773
                     /locus_tag="PLO65_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HRD27528.1"
                     /translation="MGWTDDRVATLKKLWLDGLSASQIAKQLGGVTRNAVIGKVHRLG
                     LSGRAAPSQPARPVFK"
BASE COUNT         1371 a         2948 c         3093 g         1361 t
ORIGIN      
        1 gggcctgacc atcctggagg tgaccgacgg ggccgggcgc aagctggcct ggaccctggg
       61 cgccgccgag ggcgacaagg gggcgccgct gaccgtgcag ctggccgggg cgacgcgggt
      121 ggtggtggcc tatcgctcgg cgccgggggc cctggcgctg cagtggctgc cgcccgagct
      181 gaccgccggc aaggtcaagc cgttcctcta cagccagggc cagccgacgc tgaaccgcag
      241 ctggatcccg acccaggaca gccccggcgt gcgccagacc tggaccgcgc ggatcgtcgt
      301 gcccgagggg ctgacggcgg tgatgagcgg cgagaagctg acccctgggg gcgagccggc
      361 cggaccgggc cggcgggcgt tccgcttccg catgacccat ccgaccgcgc cctatctgat
      421 cgccctggcc gccggggaca tcgccttcca ggcgctgggc ccgcgcaccg gcgtctatac
      481 cgagccggcg atgatggccc gggccgccgc cgagctggtc gacaccgaga agatggtcga
      541 ggctgccgag gcgctctacg gcccctaccg ctggggccgc tacgacgtgc tggtgctgcc
      601 gccggccttc ccctacggcg gcatggaaaa caccaccctg accttcctga ccccgacctt
      661 catcaccggc gaccgggcca acgtcgggct ggtggcgcac gagctggcgc acagctggtc
      721 gggcaacctg gtgaccaacg ccacctggga cgacatctgg ctcaacgagg gcttcaccag
      781 ctacgtcgag aaccggatga tggaggcgct ctacggcccc gaacgggccg ggatcgaggc
      841 cgacctgagc tgggacgagc tgcagcgggc cgtggccagc cggccggcgg ccgaggtgac
      901 cctgttcggc gccggcaccg gggtcgagta catcaagggc gaggccttcc tgcgcacgct
      961 ggagcacgcc ttcggccgcg accgctggga cgcctatctg cgcggctatt tcgaccgttt
     1021 cgccttccag ccgcagacca ccgccggctt cctcgccgac ctgcgcgagc acctggtcaa
     1081 gggcgacgcc gcgcttgagg cgaagctgca gctcgacgaa tgggtctacc ggcccggcat
     1141 cccgtcgggg gcctggcacg aggcctcgcc ggccctgctg acggtcgacg cggcgcggac
     1201 cgccttcctt ggcggcggcc cggccgcggc catccccggc aagggctgga cgacccagga
     1261 gtggaagcgg ttcctcgacg gcctgccgcg gcggctgacc ccggcgcagc tggccgggct
     1321 ggacgacgcc ttcggcctgt cggactcgac caacgcctat gttcgctcgg cctggctgga
     1381 gctggtggtg gccaaccgct acgccccggc cctgccgtcg ctgcagacct tcctggccag
     1441 caacggccgg ggcctgttcc tcaacccgct gtacaacggg ctgctgaagg acaaggcctg
     1501 gggcctgccg ctcgcgcggc ggtatttcga ggccgcgaag agcggctatc acccggcgct
     1561 ggcggccagc atcgagaagc gcatcgcggc ggcggagatg ccgtagggag ccacctttcg
     1621 ccggtcagcc gagggtgcga ccgatgaacg taaacatccc catcaggacg ccgatgccgc
     1681 ccagcagcag ccagccctgc tgacggatcg tgtcggtgat ggcggcccgg aagctcgcga
     1741 tctccagccg caactgggcg agttcagcct tggtggccat ctcggtgcgg aggacgtgag
     1801 tgtctgcgct ggtgctctgc cggagcgact cgatgtccgc ctttgtcgct acgcgcagca
     1861 gctcaaggtc cgccctggtg gccatgtccg cgatatcagc cttgctcgcc agatgactga
     1921 tgtccggcat cgccgacgcg tgctggaaga ccgacaccac cgcctcggcc acgccttcgg
     1981 gcataccggc ttcccggagt tgcttggaag cgctgagcgt gtcgaagacg aacggagtca
     2041 tgggaacaac ctaaaagagt gttcccgttt tgtccatcca agaagtagtc gccggctccg
     2101 acggccgtca gtcgcgcggg accggccgtt ccagctcttc gagcgcctcg gtcagcacgt
     2161 ccatgcgctt ggccagggcc gtggctgcgt cgcggaggcc ccggttgtag aagcgcgggc
     2221 cgagccgctc gcccatgaag tcgagcagga actcggcctc aagaccgccg atctcgacgt
     2281 ccagctcgtc tttcaggtag ccgcgcaacg ccctggcgag cgcagcgcga tcgtcctttt
     2341 cgagcccgac cgccggcatc agccgatctt gccgaagcac caggcgagga tcgacttctg
     2401 ggcgtggatg cggttctccg cctcgtccca gaccagcgac tgcgggccgt cgatgaccgc
     2461 gtcgctgacc tcctcgccgc ggtgggccgg caggcagtgc aggaacaccg ccttgcggtc
     2521 ggccagggcc atcagatcct cgtcgacctg gtaggattcg aacgcgtcca gccgcgcctc
     2581 gccatcgacg tcgcccatcg acacccaggt gtcggtgacc acgcagtcgg cgccgcgcac
     2641 cgcctcgcgc gggtcctcgg tcatctcgat gcggccctgc tgctcggcgg cccgggccag
     2701 gtcatagagg tccgggtgat aggccgccgg gcaggcgatg ttgagcttga agccaagctt
     2761 gggcgcggcg tggatgaagc tggagcagac gttgttgccg tccccgaccc aggcgatggt
     2821 cttgcccgcg atcggcccgc gatgctcctc gaaggtcagg atgtcggcga ggatctggca
     2881 ggggtggctc ttgtcggtca ggccgttgat caccgggatg gtggcgttct gggcgaacag
     2941 ctcgacgtcc tcgtggcggt tggcgcggat catcaccgcg tcgaccatgc gcgagagcac
     3001 ccgggcggtg tcctcgatcg gctcgccgcg gcccagctgc atgtcggccg ccgtggagat
     3061 gatgcccgaa ccgccgagct ggcggatcgc cgcgtcgaag ctgaaccgcg tgcgggtcga
     3121 attcttctcg aagatcatcg ccagggtgcg gccctcggcc ggcgcgtcgg cgtcgacgcg
     3181 cgcctgggtc cagccccggc gggcggcctt gcgggcgcgg gcgtcgtcaa ggatgacccg
     3241 caggtcgtgg gcctcgagct tccagatatc gaggaagtgt ctgggactgg acatcaatta
     3301 caccgctcat cccggcgaag gccgggaccc aggtttgttg tgtctccttc cgctcgcaca
     3361 cgggctttcc gaaaaagcct gggtcccggc cttcgccggg atgagcggaa tgaggggtca
     3421 ggccgcggcc gccgccttcg cgcgcgccgc ttcgcaggtc ttctcgagct tctcgactgc
     3481 ctcgcgggct tcctcgacgg tcagcagcag cgagggcagc aggcgcacgc agttgtcgcc
     3541 gccgccggcc accagcagtt tctggtcgcg ggccatggcc atgaactcgc gattcaccgg
     3601 cgtgaccttg agtccgatca ggaagccctt gccgcgcaca tcgacgatga tgtccgggaa
     3661 acgctccttc aggcccgtga gctgctggtt gaagtagccg gccacgtcgc ggacgttggc
     3721 cagggtctcg tccttgctga tctcgtcgaa ggcggccagg ccgacggcca tggccagcgg
     3781 gttgccgccg aaggtcgagc cgtgcacgcc gacggtcatg cccttggccg cctcggccgt
     3841 ggccaggcag gcgccgaccg ggaaaccgcc gccaagcgcc ttggccacgg ccatgatgtc
     3901 cggcgcggcg tcgctggccc attcatgggc gaacagcttg cccgtgcggc ccatgcccga
     3961 ctgcacctcg tcatagatca gcagcacgcc gttttcccgg gtccaggcgc ggatctggtc
     4021 gaggtcggct tccggcatgg cgcgcgcgcc gccctcgccc tgcaccggct cgaggatcac
     4081 cgcggccgtc gtcggctgct cgatcaacgc cttcagcgca tccatctcgc cccatttggc
     4141 ctggacgtag ccgggcaggc gcgggccgaa gccctcgaca tagcccgggt tgccggcggc
     4201 ggtgatggcc gcgtagctgc ggccgtggaa cgaaccgtcg aagccgatga tgtccacccg
     4261 ctcgggatgg ccgttggcgg tgtggtactt gcgggccgtc ttcagcgcgc actcgatcgc
     4321 ctcggtgccc gagttggtga agaacaccac gtcggcgaag gtcgcgtcgg tcagggccct
     4381 ggccagcgcc tcctggcccg ggatgcggaa gatgttggag acgtgccaga gcttgtcggc
     4441 ctggaccttg agcgtctcca ccagcttcgg atgacagtgg cccaggccgt tggtggcgat
     4501 gccggcgacg caatcgagat actcgacgcc gttgacgtcc cacaggcgcg cgccgagccc
     4561 tcggtcaacc gccagcggag cgcggttgta gacgcccatg atgtggtcgg gggtcgcggt
     4621 cggagcggtc ggggtggtca aggtcgcctc ccaaaagaga agacgtcccc gcgcgaaagc
     4681 acggggacgg gaagcttaag gcttctcacc ggggagcggt gagatgtcaa gaatttgggg
     4741 ttcagcggtc ctctcccgcg agggagaggg ggaccatgcg gagcatggtg gagagggaag
     4801 cgcaggcgtt gaccggaccg gaaatgctcg ttaacattgt atttaggaag ttagccgccg
     4861 tagcggcctc ctcatggaaa ccactgagcg cacgcgaaac ctcgcaaaac gcctccgccg
     4921 acgcatgaac gaacctgagc tccgcctatg gagtcgcgtt cgcaagaacc aactcgacgg
     4981 cattcatatc cgccggcagc acccgctcgg tcactatgtg ctcgacttct tctgcgcccg
     5041 ctcgcggctg gccatcgagg tggatggcgg catgcatgat gaggaccgtg tggcgcgcga
     5101 cgaggtccgc gacgcctggt tggcgatgcg cggcgtccgc accctccgcc tgccggccag
     5161 tctggtgatg aacgacatgg aggcagcgct cgccgccatt cgacgacgac tgactactct
     5221 gtagctatcg cgcttccctc tccaccagcc tccggctggt ccccctctcc ctcacgggag
     5281 aggagtgacg ctcacgggag agagtgacga gacgcgagca ccccctacgc gaactcgggc
     5341 gtcgccgcga gggcggccag ctgggaccgg acggaggcca tgtagcgcgc cgcgccggcc
     5401 gggggcagca cctcgaccat gatgcggttg tcgatccaca gctcgaccag gtcgaacggg
     5461 ccgcggtcgg cgtgcaccgc gcgccagccc tcggcctcgc caagggcgat gacctcgtcc
     5521 gtcgacaggg cgctggtgag cgcgaaatgg atggccgtgg gaccggcggg ggcctcgcgc
     5581 gggccggacg tgaaccgggc gccgtcaatg gggccctcgc cggccgcgat gacggccgtg
     5641 tcggggatga cctcgacgcc cgtgcccgag ccgtcgcggg ccagcaccat ccagccgccg
     5701 tcgacgacgg ggaagcggac ggcgacgccg tcgatgatgc gggcgaacag ttcggcggtc
     5761 gcctgcgggt cgcgggcggg gatggagaag tgggcgatca tgggtgattt ccttcgaagg
     5821 cgcgggggcg cgcgtgcgga acgcgtcgcc tcggcgtgct ggcggtgatg gtcggtcgca
     5881 gggccgctct ccccctcggg gctgccgctg acgccgttcg ccggacatag cgccgggcgc
     5941 cgaagcaggg cacttaaagt tgtgtcatga cctgtttgtc agactcggcg gcccgccgat
     6001 ggggacagca gccccttgcc cgtcgccgat ccgggtctag acttcccccg gcaacaaggg
     6061 atcaatcgat gagcgacgcc gtcggccctg gcgatctggt gttgtgcgtc aacgccgccc
     6121 ccaaccatgt gacgggacag cccgtgccgc tcgtcgccgg cgagacctac cgggtctgga
     6181 cggtgatgga tttcgcctgc ccgtgcgggc ggtccgacgc cctgctcgat atcggcatcg
     6241 acttcgcctg gtgccagtcg cgcttccggc tactgcccaa gccgaaggcc cagagcaagc
     6301 cggtcaaggt ttcggcgccg aaggagaagg agcgggtgcg gtgaggtgca ggggacaggt
     6361 tatcctgtcc ctcgggacgg cttgaccagt ccccgaccgt cggcggggtg tcgatgttca
     6421 tgctcgggcg attgggccgg cgatcagcgg cgagcatcgg ccgcccgcgc catcgcgacg
     6481 gccagcctcg tggtccggtc caccgtggcc ggatcgacgg ccgtgatccg gtcgttcggc
     6541 gaatgaatga gcctgagcac ggccggtaca ggcagcgggt tgagccagcc ccctcgcccc
     6601 cgccgcatga aggcatccag cgcctcgacc tcggcgcgcg gcaggtagct gaacgacgtc
     6661 gccggaatgt gccgtagcca gaaagccctg tgatcgctgg acgggtagac ctcgaacggc
     6721 cgacacggcg cgttcgcggc cctgcagccg tcctcgatga caccctttag aagggcgttc
     6781 tcggcatcgc cgggcagggc gtagtacggc gtatcgccat aggcgttgat gtcgaagttg
     6841 aagatcgccg ccgcgcgccg accgtcgtcg ctcgcggcgt aggccttggc gcccagaagc
     6901 cccagttcct cctggtcgaa gaagatgatg cgcaggcgat ggttttggag cccgccgcgc
     6961 agcaggaccg cagcccgcgc gagcgccacg acagaagcgg cgttgtcgac gacgccgtcg
     7021 gccagcctgc cgtccttcag ccgcctggcg tcgtagtggg cggtgagaag aatgtccctg
     7081 tcgccctcgc ccagggtgat gatgacgtta tggccctcgc cggccgcccg cttgagggtg
     7141 aacggccgga cctcgtgctc aaggccgaga tcggcaagca ttgcggtcag atggtccagc
     7201 cgggcccttg gcgtgggctg cgcgaacccg gcgacccatt ccgcggtggc cgtcggcggc
     7261 ggcgacgatg cggccgatcc gacggacagt acggtcgcaa gtgcggcgat gacggcctgc
     7321 ttcatgcccc tcggaatggc cctaggtctt cagccgccag cccgtcacga acacccgcca
     7381 ggaccacagg aacagggcca ccgccaggac gccggtcagc accacgccca cggtcaggtc
     7441 gccgtccgcc tcgccgatga agccgtagcg gaagccgtcg atcagataga agaacgggtt
     7501 gaaatggctg gccgtgcgga acggctcggg cagtttgtcg acgaggtaga aggtgcccga
     7561 caggaaggtc atcggcatga tcaggaagtt ggtgatcgcc gccaggtggt cgaacttctc
     7621 cgaccacagg cccgtggcga tgccgaccag gcccatcagc agcgaggcgc cgacggcgaa
     7681 atagatcacc gcccagggat gggcgacctg cagctgggtg aagggcagga tggcgatggt
     7741 cagggccgtc accgcgccga cgatcacccc gcgcgtcgcc gcgccgaggg tgaaggccag
     7801 cacctgctcc agcgccgaca gcggcggggt gagaaagtcc ggcgtcaggc ccatcatctt
     7861 ggcctgcagc aggctcgacg acgaattggc gaaggcgttg ttgatcatgg ccatcatgat
     7921 caggcccggc gccacgaagg ccgagaaggg cgtgccgtgg atcggcggcg ccgcgccgcg
     7981 cacggccacc acgaagacca tcatatagag cagggtggtg accaccggcg cggcgatggt
     8041 ctgggtgccc accttccaga agcgacggac ttccttgagg tagagggtgg aaaagcccgt
     8101 ccagttgaag ccggcgtagt cgcgcggctg cggcggaagg acgctctcgg ccatgtcttt
     8161 catgacgccc cagatgcgcc cgggcatggg aaagtgcaag acgtcggacg ccccttctcc
     8221 cttcctcctc gatggaggaa gggccggggg tggtggtgtg gccatggtct tgcgggacca
     8281 gcgttggctg gcgccggcgc ctgtcagagc ccttcgccca acccagcggc cacaccacca
     8341 cccaaccctc ctccatcgag gaggagggat tttcgtgggc ggcgccaccc cggtgcgagc
     8401 atcccggatt caccgggata cccatggatt caacatctag ttcctgtgga cagcgcccgg
     8461 gcgcctattg tgtccgttaa cagaaggttt acgatatgtt gtaggcaagg cgtccagcgg
     8521 gcgccccgaa cacaatatgt ggggtcgacg gcgcgggggc gtttgtcgcc cgtgcagtag
     8581 aaagggttgg atcatgggct ggaccgacga tcgcgtggcg acgctgaaga agctctggct
     8641 cgacggcctt tcggccagcc agatcgccaa gcagctgggc ggggtcaccc gcaacgcggt
     8701 catcggcaag gtccaccggc tgggcctgtc gggccgcgcc gcgccctcgc agccggcccg
     8761 cccggtgttc aag
//
LOCUS       DAORNX010000065         8730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5973_length_8730_cov_7.512507, whole genome shotgun sequence.
ACCESSION   DAORNX010000065 DAORNX010000000
VERSION     DAORNX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5973_length_8730_cov_7.512507"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLO65_04425"
     CDS             <1..593
                     /locus_tag="PLO65_04425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRD27529.1"
                     /translation="TLTADDIVVSNGSCTTNALAPVAKVLNDLFGIERGYMTTIHAYT
                     GDQPTLDTMHSDLYRARAAALSMIPTSTGAAKALGLVLPELKGKLDGSSIRVPTPNVS
                     VVDLKVVAAREVTVEEINKALLAASEGPMKGVLATTNEPLVSMDLNHVDASSTAALKQ
                     TQVVDGKLARVLSWYDNEWGFATRMSDTALVMAKFL"
     gene            728..1918
                     /locus_tag="PLO65_04430"
     CDS             728..1918
                     /locus_tag="PLO65_04430"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRD27530.1"
                     /translation="MTFRSLETADLAGKRVLVRVDFNVPMEGGRVTDDTRLRSALPTI
                     HYLSSRGAKVILLAHFDRPKGKRVPEMSLKPVVAPLSALLEQPVEFGEDCMGEAAFEA
                     VNRLENGGVALLENLRFHPGEEKNDPAFVTELGAIGDVYVNDAFSAAHRAHASTEGIA
                     RLLPSYPGLAMQRELEMLDAALGKPVKPVIGIVGGAKVSTKLDLLNNLVRKLDYLAIG
                     GGMANTFLAAQGHDVGGSLCEHDLGDTAREIMASARAAGCELLLPVDVVVAREVKPGT
                     ATRTCGLDDVKAGDLILDAGPATTARLVETMERCRTLIWNGPLGVFEVPPFDTATVAA
                     ARHAAALAQAGKLVAVAGGGDTVSALNHAGVAGEMTFVSTAGGAFLEWMEGKALPGVE
                     ALGA"
     gene            1996..3081
                     /gene="fba"
                     /locus_tag="PLO65_04435"
     CDS             1996..3081
                     /gene="fba"
                     /locus_tag="PLO65_04435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518732.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRD27531.1"
                     /translation="MARITLRQLLDHAAEHDYGVPAFNINNMEQGLAIMEAAEAVDAP
                     VIIQASRGARSYANDIMLARLIDALVEIYPNIPVCMHQDHGNGPATCATAIQYGFTSV
                     MMDGSLMEDAKTPASYDYNVEVTRKVVEMAHSCGVSVEGELGVLGSLETGMGEAEDGH
                     GFEGKLDHSALLTDPDQALDFVRETRVDALAIAMGTSHGAYKFTRKPDGDILAMNVIE
                     AVHKRLPDTHLVMHGSSSVPQDLQDIINEFGGEMPQTWGVPVEEIQRGIKLGVRKINV
                     DTDNRMAMTGAIRKVLMQKKGEFDPRAYLKPAKEAMRAVCKARFEQFGAAGWAGKIRP
                     ISTAVMAKRYAAGELDPQIGVSKAAAE"
     gene            complement(3085..3720)
                     /locus_tag="PLO65_04440"
     CDS             complement(3085..3720)
                     /locus_tag="PLO65_04440"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HRD27532.1"
                     /translation="MKPVWIVVVAACGLLAGSCASLSEDQCLAGDWAGIGYSDGANGY
                     GTGRFGDHVKACEKYGVVPDQSVYMTGRARGLTVYCQPGRGFQVGRQGGSYGGVCPVS
                     LEGPFLAAYSDGRLVWDAQQRYDRAQSAIYDAERRADEIETKMREAEAKIADPATTDD
                     EKKALRERLKALRDDRYSAGEDRRQAIDLRDQADRDLSRLRARFASVYGGW"
     gene            complement(3795..5441)
                     /locus_tag="PLO65_04445"
     CDS             complement(3795..5441)
                     /locus_tag="PLO65_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27533.1"
                     /translation="MNQQSGAPRQSHAMVIGGASVGTIFEWYDFYLYGSLAGFITKHF
                     FSGVNETTGYIFALLAFAAGFAVRPLGALVFGRLGDLWGRKNTFLVTMLLMGLSTFVV
                     GLLPSYDTIGVAAPVLLIAMRLVQGMALGGEYGGAATYVAEHAPRGRRGLYTSFIQTT
                     ATLGLFLSLIVILLVRTNIGEDAFGAWGWRVPFLASILLLGVSLWIRLRLNESPVFQR
                     MIDEGKGTRAPLTESFLRWPNLKLVLLALVGLTAGQAVVWYTGQFYALFFLEKMLKVD
                     GALTNILVAVALLLGTPFFILFGWLSDRIGRKPIILAGCLIAALTFFPLFRTLTVAAN
                     PALAAATAHAPVTVIADPATCSFQFDPVGKTAFRSSCDIAKSALASAGVSYRTREAVA
                     GSTATVEIGGARVAAFEGAGMATKAFNAEQMAWRKTLGAALHAAGYPDKADPARVDRP
                     MVVAVLFALILLVTMVYGPIAAALVEMFPARIRYTSMSLPYHIGNGWFGGFLPTTAFA
                     MVAATGDIYYGLWYPVVVAAVTAVVGLLFIREMKDADIEG"
     gene            complement(5492..6049)
                     /locus_tag="PLO65_04450"
     CDS             complement(5492..6049)
                     /locus_tag="PLO65_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRD27534.1"
                     /translation="MIVEAARDAIDEIANSRGRSTRHLLVGAGLTVGAVLASALIARA
                     NSPTADHPQIERRYNRLKQPSFSPPKAAFAVIWPPLFLALTLSGLRVWNAPSSPARTQ
                     ALTLWGVVQALNAVWMALGPRRLGGQLTAAVVSLGTSAAYAWRARQVDLPAAGMVAPY
                     VGWIGFANVLTGELWRRNHANPTLH"
     gene            complement(6125..6607)
                     /gene="bfr"
                     /locus_tag="PLO65_04455"
     CDS             complement(6125..6607)
                     /gene="bfr"
                     /locus_tag="PLO65_04455"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRD27535.1"
                     /translation="MKGDAAIIRLLNAVLTNELTAVNQYFLHARMYQDWGFARLGRIT
                     YDESIGEMKHADKLIKRILFLEGLPNLQDLNKLKIGETVPECLAADLAVEVGGRDTLV
                     AGVGQCETANDYVSRQILKEILQDTEEHIDFLETQLNLLRTLGEQNYLQSALGELEGG
                     "
     gene            complement(6696..6881)
                     /locus_tag="PLO65_04460"
     CDS             complement(6696..6881)
                     /locus_tag="PLO65_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD27536.1"
                     /translation="MYVCNCNGIRERQVRAAIEAGATRPAEVFRAHGCRPQCARCVCE
                     MREMIEQSREALRFAAE"
     gene            7011..7655
                     /gene="thiE"
                     /locus_tag="PLO65_04465"
     CDS             7011..7655
                     /gene="thiE"
                     /locus_tag="PLO65_04465"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRD27537.1"
                     /translation="MTADCRLYLITPPRLDDLAGFAHVLAAALDAGDVAALQIRLKDA
                     PDDVIAAAVEVLGVIARSRDVAVILNDRPDLAARLDCDGVHVGQDDMSCAEARRIVGK
                     DRMVGVTCHDSRHLAMEAAEAGADYVAFGAFFPTSTKDAPTRADPEILTIWQETMSLP
                     CVAIGGITAGNAAGLAAAGADFIAVSAGVWRHPEGPAAAVRALNAAIAEGLKER"
     gene            7832..8398
                     /gene="efp"
                     /locus_tag="PLO65_04470"
     CDS             7832..8398
                     /gene="efp"
                     /locus_tag="PLO65_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRD27538.1"
                     /translation="MKINGNTIKPGMVLQHDGGLWVCVKAEHVKPGKGGAFAQVELKN
                     LIDGRKLNERFRSDDKVERVTLDQRDNTFLYAEGDMLVFMDSENYEQISLHRDFVGED
                     QVRFLQDGMKVSVQFHEERPISIALPEHVVLTIAEADATVKGQTASSSYKPAKADNGM
                     RILIPPYMDAGERVLVSTETGEYLRRAD"
     gene            8400..>8730
                     /locus_tag="PLO65_04475"
     CDS             8400..>8730
                     /locus_tag="PLO65_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD27539.1"
                     /translation="MSTASALLTVMIDAARKAAKGLARDFGEVAELQVSKKGAGDFVT
                     AADIKAEQVLFEALTKARPGYSFLGEERGLIEGTDKTHRWIVDPLDGTTNFLHAIPHF
                     AVNIALER"
BASE COUNT         1454 a         3036 c         2979 g         1261 t
ORIGIN      
        1 agaccctgac cgccgacgac atcgtcgtct ccaacggcag ctgcaccacc aacgctctgg
       61 ccccggtggc caaggtgctg aacgacctgt tcggcatcga gcgcggctat atgaccacaa
      121 tccacgccta caccggcgac cagccgacgc tcgacaccat gcacagcgac ctctatcgcg
      181 cccgcgccgc ggcgctgagc atgatcccga cctcgaccgg cgcggccaag gcgctgggcc
      241 tggtgctgcc cgagctgaag ggcaagctgg acggctcctc gatccgcgtg ccgaccccca
      301 acgtctcggt ggtcgacctg aaggtcgtcg ccgcgcgcga ggtgacggtg gaggagatca
      361 acaaggccct gctcgccgcc tcggaaggcc cgatgaaggg cgtgctggcg accaccaatg
      421 agccgctggt ctcgatggac ctgaaccacg tcgacgccag ctccacggcc gccctcaagc
      481 agacccaggt cgtcgacggc aagctcgccc gcgtgctcag ctggtacgac aacgaatggg
      541 gcttcgcgac ccgcatgagc gacaccgcgc tggtgatggc gaagttcctg tagcacccaa
      601 actccgctca tcccggcgaa ggccgggacc caggcttgtt tggctgactt ccggcgcagg
      661 tgcgtttcag aaaaagcctg ggtcccggcc ttcgccggga tgagcgggaa attatgagat
      721 gcactccatg accttccgct ccctcgaaac cgctgatctc gccggcaagc gcgtcctcgt
      781 ccgcgtggac ttcaatgtgc cgatggaggg gggcagggtc accgacgaca cccgcctgcg
      841 ttcggccctg ccgacgatcc actacctgtc gagccggggc gccaaggtca tcctgctggc
      901 ccacttcgac cggcccaagg gcaagcgggt gccggagatg agcctgaagc cggtggtcgc
      961 gcccctgtcg gccctgctgg agcagccggt cgagttcggc gaggattgca tgggcgaggc
     1021 agccttcgag gccgtgaacc ggcttgagaa cggcggcgtg gccctgctgg agaacctgcg
     1081 cttccatccg ggcgaagaga agaacgaccc cgccttcgtc accgagctcg gggccatcgg
     1141 cgacgtctac gtcaacgacg ccttcagcgc cgcccaccgg gcccatgcct cgaccgaggg
     1201 catcgcgcgc ctgctgccgt cgtatccggg cctggccatg cagcgcgagc tggagatgct
     1261 cgacgcggcg ctcggcaagc cggtcaagcc ggtgatcggc atcgtcggcg gggccaaggt
     1321 ctcgaccaag ctcgacctgc tcaacaacct cgtgcgcaag ctcgactacc tggccatcgg
     1381 cggcggcatg gccaacacct tcctggccgc ccagggccat gacgtcggcg gttcgctctg
     1441 cgaacacgac ctgggcgaca cggcgcgcga gatcatggcc tcggccaggg cggcgggctg
     1501 cgagctgctg ctgccggtcg atgtggtcgt cgcccgcgag gtcaagccgg gcacggccac
     1561 ccgcacctgc ggcctggacg acgtgaaggc cggcgacctg atcctcgacg ccggcccggc
     1621 gaccaccgcc cggctcgtcg aaaccatgga gcgctgcagg acgttgatct ggaacggccc
     1681 gctgggcgtg ttcgaggttc cgccgttcga caccgccacc gtggccgccg ccagacatgc
     1741 ggccgcgctg gcgcaggccg gcaagctggt cgctgtggcc ggcggcggcg acacggtctc
     1801 ggccctcaat cacgccggcg tggcgggcga gatgaccttc gtctcgacgg ccggcggcgc
     1861 cttcctggaa tggatggaag gcaaggcgct gccgggcgtc gaggccctcg gggcctgacc
     1921 ggcgggtgac accgcgggcg gtcccattta aagaacagca gaacctgaaa tcgcgcgaac
     1981 aaggatcctg acgtcgtggc ccgcatcact ctccgacagc ttctcgacca cgccgccgag
     2041 cacgactacg gcgtgcccgc gttcaacatc aacaacatgg aacagggcct ggcgatcatg
     2101 gaggcggccg aggccgtcga cgcgccggtg atcatccagg cgtcgcgcgg cgcgcgcagc
     2161 tacgccaacg acatcatgct ggcccggctg atcgacgccc tggtcgagat ctaccccaac
     2221 atcccggtct gcatgcacca ggaccacggc aacggcccgg ccacctgcgc caccgccatc
     2281 cagtacggct tcaccagcgt gatgatggac ggctcgctga tggaggacgc caagaccccg
     2341 gcctcctacg actacaacgt cgaggtcacc cgcaaggtgg tcgagatggc ccacagctgc
     2401 ggcgtctcgg tcgagggcga gctgggcgtg ctgggcagtc tcgagaccgg catgggcgag
     2461 gccgaggacg gccacggctt cgagggcaag ctcgatcatt cggccctgct gaccgacccc
     2521 gaccaggcgc tggacttcgt tcgcgagacg cgcgtcgacg ccctggccat cgccatgggc
     2581 accagccacg gcgcctacaa gttcacccgc aagcccgacg gcgacatcct ggcgatgaac
     2641 gtcatcgagg cggtgcacaa gcgcctgccc gacacccacc tggtgatgca cggctccagc
     2701 tcggtgccgc aggacctgca ggacatcatc aacgagttcg gcggcgagat gccccagacc
     2761 tggggcgtgc cggtcgagga gatccagcgc ggcatcaagc tgggcgtgcg caagatcaac
     2821 gtcgacaccg acaaccgcat ggccatgacc ggcgcgatcc ggaaggtgct gatgcagaag
     2881 aagggtgagt tcgacccgcg cgcctatctg aagcccgcca aggaggccat gcgcgcggtc
     2941 tgcaaggccc gcttcgagca gttcggcgcg gccggctggg ccggcaagat ccggccgatc
     3001 agcaccgccg tgatggccaa gcgctacgcc gcgggcgagc tcgaccccca gatcggcgtc
     3061 agcaaggccg cggcggagta ggggtcacca tcccccgtac accgaggcga accgggccct
     3121 caggcggctc aggtcgcggt cagcctggtc gcgcaggtcg atggcctggc ggcgatcctc
     3181 gccggcgctg tagcggtcgt cgcgcagggc cttgaggcgc tcgcgcaggg ccttcttctc
     3241 gtcatcggtg gtggcggggt cggcgatctt cgcctcggcc tcgcgcatct tcgtctcgat
     3301 ctcgtcggcg cggcgctcgg cgtcgtagat cgccgactgg gcgcggtcgt agcgctgctg
     3361 ggcgtcccag acgaggcggc cgtccgagta ggccgccagg aaggggccct ccagcgagac
     3421 cgggcagacc ccgccgtagc tgccgccctg gcggccgacc tggaagccgc ggccgggctg
     3481 gcagtagacc gtcaggccgc gcgcccgacc ggtcatgtag accgactggt cgggcaccac
     3541 gccgtacttc tcgcaggcct tcacatggtc gccgaaccgg ccggtcccgt agccgttggc
     3601 gccgtcgctg tagccgatgc cggcccagtc gccggccagg cactggtcct cgctgaggct
     3661 ggcgcaactg ccggccagca ggccgcaggc cgcgacgacg acaatccaga ccggtttcat
     3721 gtgctcggct ccccgacgac ccgggtcgca ggatttcgcg tttcgccgcc ggtgggaagg
     3781 tccgaagagg ggtatcagcc ctcgatgtcg gcgtccttca tttcccggat gaacagcaag
     3841 ccgaccacgg cggtcaccgc cgcgaccacc accgggtacc agaggccata gtagatatcg
     3901 cccgtggcgg cgaccatggc gaaggcggtg gtcggcagga agcccccgaa ccagccattg
     3961 ccgatatggt agggcaggct catcgaggtg tagcggatcc gcgccgggaa catctccacc
     4021 agggccgcgg cgatcgggcc gtagaccatg gtgaccagca ggatcagggc gaacagcacc
     4081 gccaccacca tcggccggtc gacccgcgcc gggtcggcct tgtccggata gccggcggcg
     4141 tgcagggcgg ccccgagagt ctttcgccag gccatctgtt cggcgttgaa cgccttggtc
     4201 gccatgccgg cgccttcgaa cgccgcgacc cgcgctccgc cgatctccac cgtcgccgtc
     4261 gagccggcca ccgcctcgcg ggtgcggtag gagaccccgg ccgaggccag ggccgacttg
     4321 gcgatgtcgc aggacgaacg gaaggcggtc ttgccgaccg ggtcgaactg gaacgagcag
     4381 gtcgccggat cagcgatcac ggtcaccggc gcatgggccg ttgccgcggc cagggccgga
     4441 ttcgccgcca ccgtcagggt ccggaacagc gggaagaagg tcagggcggc gatcaggcag
     4501 ccggccagga tgatcggctt gcgcccgatc cggtcgctga gccagccgaa caggatgaag
     4561 aacggcgtcc ccagcagcag ggccacggcc accaggatgt tggtcagggc gccatcgacc
     4621 ttgagcatct tctcgaggaa gaacagggcg tagaactgcc cagtgtacca gaccacggcc
     4681 tggccggcgg tcagtccgac aagggccagc agcaccagct tgaggttggg ccagcgaagg
     4741 aacgactcgg tcagcggcgc ccgggtcccc ttgccctcgt cgatcatccg ctggaacacc
     4801 gggctctcgt tgagcctcag gcggatccac agggagacgc ccagcagcag gatcgaggcg
     4861 aggaacggca cgcgccagcc ccaggcgccg aacgcgtcct cgccgatgtt ggtgcgcacg
     4921 agcaggatca cgatcagcga caggaacagg cccagggtcg ccgtggtctg gatgaagctg
     4981 gtgtagagcc cgcgccggcc ccggggggcg tgttcggcga cgtaggtcgc ggccccgccg
     5041 tactcgccgc ccagcgccat gccctggacc aggcgcatgg cgatcagcag caccggcgcg
     5101 gccacgccga tggtgtcgta ggacggcagc aaaccgacca cgaaggtcga caggcccatc
     5161 agcaacatcg tcaccaggaa ggtgttcttg cggccccaca ggtcgccgag ccggccgaac
     5221 accagggcgc cgagcggccg caccgcgaat ccggcggcga aggccagcag ggcgaagatg
     5281 tagccggtgg tctcgttgac cccggagaag aagtgtttgg tgatgaaccc ggccagcgag
     5341 ccgtagaggt agaagtcata ccattcgaaa atggtcccga cggacgcgcc gccgatgacc
     5401 atggcgtggc tctgcctggg cgcgcccgac tgctggttca tcccgtcccc ctccggtttg
     5461 gccggaggct agcgcgggac gggggtcggg ctcaatgcag cgtcgggttg gcgtggttgc
     5521 gccgccagag ctcgccggtc agcacgttgg cgaagccgat ccagccgaca tagggcgcga
     5581 ccatgccggc ggccgggaga tccacctggc gcgcgcgcca ggcgtaggcg gccgaggttc
     5641 ccagcgagac caccgcggcc gtcagctggc cgcccagacg ccgcgggccg agcgccatcc
     5701 agacggcgtt gagggcctgg accacgcccc acagggtcag ggcctgggtg cgggccgggc
     5761 ttgagggcgc gttccacacc cgcaggcccg agagggtcag ggccaggaac agcggcggcc
     5821 agatcaccgc gaaggcggcc ttgggcggtg agaaggacgg ctgcttcagg cggttgtacc
     5881 ggcgttcgat ctgcggatgg tcggcggtgg gcgaattggc ccgcgcgatc agcgccgagg
     5941 ccagcacggc cccgaccgtc aaacccgcgc cgacaagcag gtgacgggtc gagcggcccc
     6001 ggctgttggc gatttcatcg atggcgtcgc gcgcggcttc gacgatcatg gcggcctcct
     6061 cgctatcaag cgggaagcgg ttcggcggcg gggaggttcc agggggggcg gttcgacgcc
     6121 ggtcctaacc gccctcgagc tcgcccaggg cgctctgcag gtagttctgt tcacccagcg
     6181 tccgcaacag gttcagctgg gtctcgagga agtcgatgtg ctcctcggtg tcctgcagga
     6241 tttccttgag gatctggcgg ctgacgtagt cgttggcggt ctcgcactgg ccgaccccgg
     6301 cgaccagggt gtcgcgcccg ccgacctcga cggccaggtc ggcggccagg cattccggca
     6361 ccgtctcgcc gatcttcagc ttgttcagat cctgcaggtt gggcaggcct tcgaggaaca
     6421 ggatgcgctt gatcagcttg tcggcgtgtt tcatctcgcc gatcgactcg tcataggtga
     6481 tccttcccag ccgcgcgaag ccccagtcct ggtacatccg ggcgtgcagg aaatactggt
     6541 tcaccgccgt cagctcgttg gtgagcactg cgttcagcag acggatgatg gccgcatcgc
     6601 ccttcatggc gcgctccttc ctgttcgagt cggcccgacc ctgaaccggg cgtcgtcctg
     6661 tcgcaaccgt gacgtcgccc tgacccgcgc gatcgctact cggcggcgaa gcggagggct
     6721 tcccgcgact gctcgatcat ctcgcgcatc tcgcagacgc agcgcgcgca ttgcggccgg
     6781 cagccgtggg cgcggaacac ctcggccgga cgcgtggcgc ccgcctcgat ggcggcgcgg
     6841 acctgccgct cgcgaatgcc gttgcagttg cagacgtaca aggagatggc ctcgacttga
     6901 gaatgatacg catgtagccg ttcccgggcg ccgttgcaac tcatttgcat ctggccccgg
     6961 ggcgcggttc ggctgtcccg ccggagcggc ggctggtgta gagacgggcc atgaccgctg
     7021 attgccgcct ctacctgatc actccgcccc gtctcgacga cctggccggc ttcgcccacg
     7081 tgctggccgc ggcgctcgac gccggcgacg tcgccgccct gcagattcgc ctcaaggacg
     7141 cgcccgacga cgtcatcgcc gcggcggtcg aggtgctcgg cgtcatcgcc cgttcgcgcg
     7201 acgtggcggt gatcctcaac gaccggccgg acctcgccgc tcgcctcgac tgcgacgggg
     7261 tgcatgtcgg gcaggacgat atgagttgcg ccgaggcccg tcgcatcgtc ggcaaggacc
     7321 gcatggtcgg ggtgacctgc cacgacagcc gccacctggc catggaggcc gccgaggccg
     7381 gcgccgacta cgtcgccttc ggggccttct tcccgacaag caccaaggac gccccgacgc
     7441 gggccgaccc cgaaatcctg acgatctggc aggagaccat gtcattgccc tgcgtcgcca
     7501 tcggcgggat cacggccggc aacgccgcgg gcctggcggc ggccggggcc gacttcatcg
     7561 ccgtcagcgc cggggtgtgg cggcatccgg agggcccggc cgccgcggtc agggcgctca
     7621 acgcggcgat cgccgagggg ctcaaggagc ggtgatgttg cgtggttcga cacgcgcctt
     7681 cggcgctgct ctccatgacg tacggaaatg cacgtcatcc tgagcaggcc cggagggccg
     7741 tgtcgaagga cgcacccaag cccgccttgc tatcgacccg ccccgccgtt aggttccgcc
     7801 gccctttccc ggcccttcgg cgacccagtt catgaagatc aacggcaaca ccatcaagcc
     7861 tggcatggtg ctccagcacg acgggggcct gtgggtctgc gtcaaggccg agcacgtcaa
     7921 gccgggcaag ggcggggcct tcgcccaggt ggagctgaag aacctcatcg acggccgcaa
     7981 gctgaacgaa cgcttccggt ccgacgacaa ggtcgaacgc gtcacgctcg accagcgcga
     8041 caacaccttc ctgtacgccg agggtgacat gctggtgttc atggactccg aaaactacga
     8101 gcagatcagc ctgcacaggg acttcgtcgg cgaagaccag gtgcgtttcc tgcaggacgg
     8161 catgaaggtc agcgtgcagt tccacgagga gcgaccgatc agcatcgccc tgccagaaca
     8221 tgtggtgctg accatcgccg aggccgacgc cacggtgaag ggccagaccg cctcgtcctc
     8281 ctacaagccg gcaaaggccg acaacggcat gcgcatcctc attcccccct acatggacgc
     8341 cggtgaacgc gtgctggtct cgaccgagac cggcgaatac ctccggcgcg ccgactgacg
     8401 tgtccacagc ctccgccctc ctcaccgtca tgatcgacgc cgcccgcaag gcggccaagg
     8461 gtctcgcgcg cgacttcggc gaagtggccg agctgcaggt ctccaagaag ggggccggcg
     8521 acttcgtcac cgccgccgac atcaaggccg aacaggtgct gttcgaggcc ctgaccaagg
     8581 cccgccccgg ctacagcttc ctcggcgagg agcggggcct gatcgagggc accgacaaga
     8641 cccaccgctg gatcgtcgac ccgctcgacg gcacgaccaa cttcctgcac gccatcccgc
     8701 acttcgcggt caacatcgcg ctggagcgcg
//
LOCUS       DAORNX010000066         8714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_5995_length_8714_cov_7.829311, whole genome shotgun sequence.
ACCESSION   DAORNX010000066 DAORNX010000000
VERSION     DAORNX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_8714_cov_7.829311"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PLO65_04480"
     CDS             <1..1217
                     /locus_tag="PLO65_04480"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRD27540.1"
                     /translation="KIVKVQRQAMKVGAPIIGLFDAGGARIQEGVDSLAGYADIFLEN
                     VLASGVVPQISVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPDVVKTVTNEIVTA
                     EELGGARVHAAKSGVAEGAFENDLEAMTQVRRLVDFLPSSNREKAAGRESFDEAFRDE
                     MSLDTLVPANPNKPYDMKELILKIVDEADFFEISSEWAKNIICGFARMDGETVGIVAN
                     QPQVLAGVLDIDSSRKAARFVRFCDAFNIPLVTLVDVPGFMPGTKQEYGGLIKHGAKL
                     LFAYAEATVPKVTVITRKAYGGAYDVMSSKHLRGDVNYAWPTAEIAVMGAKGAVEIIF
                     RAEMNDPEALAAREAEYKDRFANPFVAASRGYIDDVIKPHGTRRRIVRALRSLKGKQL
                     SNPWKKHDNIPL"
     gene            complement(1223..1588)
                     /locus_tag="PLO65_04485"
     CDS             complement(1223..1588)
                     /locus_tag="PLO65_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27541.1"
                     /translation="MELRKQAGLTRPSLKPFDMVARIGQWTPYALPYACLQCRKSFKR
                     RVSREAGTATETGICPECGAETVRLSRQFKPPKSTDFAQWRKVEALVQAGFRFERLGE
                     PYPETLAEVPAFVARHRHT"
     gene            2285..2545
                     /locus_tag="PLO65_04490"
     CDS             2285..2545
                     /locus_tag="PLO65_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27542.1"
                     /translation="MTRPRLGRRAMDALYAQATAEHAALEEEAAINRINELGGIARLA
                     LMIQSSRSPQTHPGLGELMARKEACDRARPRKPYAMPPAMKS"
     gene            complement(2515..2823)
                     /locus_tag="PLO65_04495"
     CDS             complement(2515..2823)
                     /locus_tag="PLO65_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRD27543.1"
                     /translation="MTVQCGLSAWIARGAKRFVSQVKSVNVEISVVLGRSTLPMSQLL
                     RMGRGAVIPLDAKASDEVWILANNHPVARGEIQINDDRISISVTRAADVYDFMAGGMA
                     "
     gene            2822..3586
                     /gene="lipB"
                     /locus_tag="PLO65_04500"
     CDS             2822..3586
                     /gene="lipB"
                     /locus_tag="PLO65_04500"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRD27544.1"
                     /translation="MLDQPLNPGPPAADSGPIFGRADGLPVVWAVSRAPVAYPDAVAA
                     MEARAEAIARGEAGELVWLLEHPPLYTAGVSARAADLIAPDRFPVFESGRGGQYTYHG
                     PGQRVAYVMLDLGKRSKDVRAFVAALESWVIEALGRFNLEGQIREGRVGVWVERKGPG
                     WSREDKIAAIGVKLRRWVSFHGVSLNVEPDLTHFDGIVPCGVTEHGVTSLVDQGLPVT
                     MDEADAALKAAFERVFGPVISVPSPLAGEGVGGADG"
     gene            complement(3703..4059)
                     /locus_tag="PLO65_04505"
     CDS             complement(3703..4059)
                     /locus_tag="PLO65_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27545.1"
                     /translation="MRATIAFVLALAVTAPAVAAEIPPEAQPGVDAVKSLLKSAKSAS
                     FRKIKVNAAGDVCAMVTPSAASDDIAFVWTKATGQVWINESPENGYSEFVWGSPMLKR
                     STDRADYQAWKACQKG"
     gene            complement(4074..4478)
                     /locus_tag="PLO65_04510"
     CDS             complement(4074..4478)
                     /locus_tag="PLO65_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRD27546.1"
                     /translation="MSGQIDWVELFFSSTGRVARTPFLIAAAVLIAIALVYEASMGPT
                     MHLLTGWFIYPGLLFSGACVLSKRLHDRGRSGWLAAIIILALIVVWPIPAGFLDFAFS
                     VVIVWAAVELGVMAGEQGANRYGPNPVKTVTL"
     gene            complement(4521..4940)
                     /locus_tag="PLO65_04515"
     CDS             complement(4521..4940)
                     /locus_tag="PLO65_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRD27547.1"
                     /translation="MYDLYRFSGRTGRLGFALTQIGVFTAAYVFTFLIIVLTGAGDES
                     GAHITGASQGAVAALMAFLLLNGAWISLAAAVRRCHDRGLSGWMLLFAMPPVLGQLWL
                     VLSLVTGEGDRAPNRYGSRAPGLPLPAADLSPRAALA"
     gene            complement(5172..7145)
                     /locus_tag="PLO65_04520"
     CDS             complement(5172..7145)
                     /locus_tag="PLO65_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRD27548.1"
                     /translation="MFSKILIANRGEIAVRVIKTCRRMGIKTVVVYSEADADSLAVEM
                     ADETVFIGPAPASESYLVADKIIAACNETGAQAIHPGFGFLSENAAFAQRCADEGIVF
                     IGPNPGAIRAMGDKIESKKFAETAGVSCVPGHIGEIDDTAHAIRISEEIGYPVMIKAS
                     AGGGGKGIRVAHSRQDVEEGFPAVRAEARSSFGDDRIFIEKFITSPRHIEIQVLGDKH
                     GNVVHLFERECSIQRRNQKVIEEAPSPLLDEATRAAMGAQAVALAKAVNYDSAGTVEF
                     VAGQDKSFYFLEMNTRLQVEHPVTELITGLDLVEQMIRSAAGEAMAFGQKDLKINGWA
                     IESRIYAEDPYRKFLPSIGRLVRYDPPVEGEHDGYVVRNDAGVREGDEISMFYDPMIS
                     KLCTWGETRLAAIDGMGRALEDFHIEGLGHNIPFLAAVMDQERFRSGKLATSYIVDEF
                     PDGFDGTTPTPSQVDMLTAAGVAMHRVLTRRAGGSDRPDWTVLVGHDSRDVTVAEAGG
                     VMTLTLGGGRTLTLSDVAWRPGKPLFRGRLDGRAFTVTVAPAAEGFVIRHRAARAKVL
                     VLSPRSAELHGMLPEKQPADTSKMIISPMPGLVVSMDVVIGQTVREGEVVCVIEAMKM
                     QNIIRAEREGVVITISAKGGDSVAADEVLVEFG"
     gene            complement(7362..7682)
                     /locus_tag="PLO65_04525"
     CDS             complement(7362..7682)
                     /locus_tag="PLO65_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005375828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRD27549.1"
                     /translation="MLTATTPTIAERPTRETLGVVSGEVILGANIFRDLFAGIRDIVG
                     GRSGSYEEVLRDAREGALREMAAQAATMGADAVVGVDLDYESLGANNSMLMVTATGTA
                     VRLG"
     gene            7730..8203
                     /gene="msrB"
                     /locus_tag="PLO65_04530"
     CDS             7730..8203
                     /gene="msrB"
                     /locus_tag="PLO65_04530"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRD27550.1"
                     /translation="MTRSAISASGFDLTPPTGAERDRLEADLTREEAEVLLHHGTEAP
                     FCGGLLGEKRPGAYCCRLCGLPLFRFETKFESGTGWPSFYAPCDPAHVGEVRDTSYGM
                     LRIETVCARCGSHQGHVFPDGPPPTRLRYCINSISLTFVGDGEPLPDPLGRGDSF"
     gene            8414..8500
                     /locus_tag="PLO65_04535"
     tRNA            8414..8500
                     /locus_tag="PLO65_04535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8448..8450,aa:Leu,seq:gag)
     gene            8558..>8714
                     /locus_tag="PLO65_04540"
     CDS             8558..>8714
                     /locus_tag="PLO65_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRD27551.1"
                     /translation="MTRDKADLIEEETLAVPAGAETAAPPEDLEDLALGDDYALNPEY
                     VDMVIDAA"
BASE COUNT         1391 a         2930 c         3009 g         1384 t
ORIGIN      
        1 agaagatcgt caaggtccag cgccaggcca tgaaggtcgg ggcgccgatc atcgggctgt
       61 tcgatgctgg cggcgcccgc atccaggagg gcgtcgacag cctcgcgggc tatgccgaca
      121 tcttcctcga gaacgtgctg gcctcgggcg ttgtgccgca gatcagcgtg atcatgggcc
      181 cctgcgccgg cggtgacgtc tattccccgg ccatgaccga cttcatcttc atggtgaagg
      241 acacctcgta catgttcgtc accggcccgg atgtggtgaa gacggtgacc aacgagatcg
      301 tcacggccga ggagctgggt ggcgcccggg tccacgcggc caagtcgggc gtcgccgagg
      361 gggcgttcga gaacgacctg gaagccatga cccaggtccg ccgcctggtg gacttcctgc
      421 ccagctccaa ccgcgagaag gcggccggtc gcgagagctt cgacgaggcc ttccgtgacg
      481 agatgagcct ggacaccctg gttccggcca atccgaacaa gccctacgac atgaaggagc
      541 tgatcctcaa gattgtcgat gaggcggatt tctttgaaat ctcaagtgaa tgggccaaga
      601 acatcatctg cggcttcgcg cgcatggatg gcgagacggt cggcatcgtc gccaaccagc
      661 cgcaggtcct ggccggggtg ctggacattg attccagccg caaggccgcc cgcttcgtgc
      721 gcttctgcga cgccttcaac atcccgctgg tcaccctggt cgatgtgccg ggcttcatgc
      781 cggggaccaa gcaggagtac ggcggcctga tcaagcacgg cgcaaagctg ctgttcgcct
      841 acgccgaggc gacggtgccc aaggtcacgg tgatcacgcg caaggcctac ggcggcgcct
      901 atgacgtgat gagctccaag cacctgcgcg gcgacgtcaa ctacgcctgg ccgacggccg
      961 agatcgcggt catgggcgcc aagggggcgg tggagatcat cttccgcgcc gagatgaacg
     1021 atcccgaggc cctggcggcc cgcgaggccg aatacaagga ccgcttcgcc aacccgttcg
     1081 tcgccgccag ccgcggctac atcgacgacg tgatcaagcc gcacggcacg cggcggcgga
     1141 tcgtgcgggc gctgcgtagc ctgaagggca agcagctcag caatccctgg aagaagcacg
     1201 acaacatccc gctgtagggg ggtcaggtgt gtcggtggcg ggccacgaag gcaggaacct
     1261 cggcgagggt ttccggatat ggctcgccca ggcgctcgaa gcggaagccg gcctggacaa
     1321 gggcttcgac cttgcgccac tgggcgaagt ccgtgctctt tggaggcttg aattgtcgtg
     1381 agagacgcac ggtttcagcc ccgcactcgg ggcagatgcc agtctcggtt gccgtgcccg
     1441 cttctctgga cacccgccgt ttgaagctct ttcggcattg aaggcaggca tagggcaggg
     1501 cgtacggcgt ccactggcca atgcgggcga ccatatcgaa cggcttcagc gaaggccggg
     1561 tgagccccgc ctgcttccgc agttccattc gccgcatctt ttctgcgttc gccatagcga
     1621 cactctagca agggcgctcg tttggtgcag caccgaagcc catgacggcg aacccgccac
     1681 accccgccct tcatctcatg gcgaggtgat ccgaccggca cgcatggcct gcatgagatc
     1741 aagggcgatc tcccttcccc catcgagggg gaagggcttc tctccggggg gcgttctacg
     1801 attggcgtcg tgtaacccct tgaaactcca tacctccgcc cgcgctatcc gaccgcgctc
     1861 ggacaatcgg cgcatcaccc cctgaaccgc cccccatact cacgccatcc ccgccgcacg
     1921 gggagggcgt atggccatgc gaccttgcgg cggcggggac ggggttcgag cggggacagg
     1981 cggcgggggg ttaccgtcgc ggtccgggga cggcgcagca cgccgcccgc ccgccagggg
     2041 cagaaccggc taagcgccaa cagcgctgcc taccggtcgc tcctgaccag acggacttcg
     2101 cggcgcgtga cggtctcacc atctctcgca agcctgccaa acagacatcc gggcgagacc
     2161 ggagcgcgcc gcatcacctc cccgcctcac ggggacatgc acttcagtgc gtgtcccctg
     2221 tttgaagtcg ttctgatgaa ggggacacgc acggaagtgc atgtccccgc gtgaaggagc
     2281 acccatgacc aggccccgcc tcggccgccg cgcgatggac gccctctacg cccaggccac
     2341 ggccgagcat gccgccctgg aggaggaggc ggcgatcaac aggatcaatg aactgggcgg
     2401 gatcgcgcgg ctggcgctga tgatccagag cagccggtcg ccgcagacac atcccgggct
     2461 gggcgaactg atggcccgca aggaggcctg cgaccgcgcg cgaccgcgaa aaccttacgc
     2521 catgccgccg gccatgaagt cgtagacgtc ggcggcgcgg gtcacggaaa tgctgatgcg
     2581 gtcgtcgttg atctggattt cgccgcgcgc gaccgggtga ttgttggcca ggatccacac
     2641 ctcgtccgag gccttggcgt cgagcgggat gaccgccccg cggcccatgc gcagcagctg
     2701 cgacatcggg agcgtcgagc gcccgaggac cacggaaatc tcgacgttga ccgacttgac
     2761 ctgactcacg aaacgcttgg cccctcgcgc aatccacgct gagagaccac attggaccgt
     2821 catgcttgat cagccgttaa atcccgggcc gcccgccgcc gacagcgggc cgattttcgg
     2881 ccgcgccgac ggattgcccg tcgtctgggc cgtgtcgcgc gcgccggtgg cctatcccga
     2941 cgccgtggcg gcgatggaag cgcgggccga ggccatcgcc aggggcgagg ccggcgagct
     3001 ggtctggctg ctcgagcatc ccccgctcta caccgccggc gtctcggcca gggccgccga
     3061 cctgatcgcg cccgaccggt tcccggtgtt cgagagcggg cgaggcggcc agtacaccta
     3121 tcacggcccc ggccagcggg tggcctacgt catgcttgat ctgggcaagc ggtcgaagga
     3181 cgtgcgggcc ttcgtggcgg cgctggagag ctgggtcatc gaggcgctcg gccgcttcaa
     3241 cctcgagggg cagatccgcg agggccgtgt cggcgtgtgg gtcgagcgca agggacccgg
     3301 ctggtcgcgc gaggacaaga tcgcggccat cggcgtcaag ctgcgccgct gggtcagctt
     3361 ccatggcgtc agcctcaacg tcgagccgga cctgacgcat ttcgacggca tcgtgccctg
     3421 cggcgtgacc gaacacggcg tgaccagcct cgtcgaccag ggcctgccgg tgaccatgga
     3481 cgaggccgac gcggcgctga aggcggcctt cgagcgggtg ttcgggccgg tgatttctgt
     3541 tccttctccc cttgcgggag aaggtgtcgg cggagccgac ggatgagggg tttctcgcgt
     3601 acagcggaat tgtcggccga aggttgaccc cggacgcggg atccgacccc tcatccgagc
     3661 cgcttcgcgg cccaccttct cccgcaaggg gagaaggggt ccctatccct tctggcaggc
     3721 cttccaggcc tggtagtcgg cccggtcggt cgaccgcttg agcatcgggc tgccccagac
     3781 gaactcggag tagccgttct ccggcgactc gttgatccac acctggccgg tggccttggt
     3841 ccagacgaag gcgatgtcgt cgctggccgc gctgggggtg accatggcgc agacgtcgcc
     3901 ggcggcgttg accttgatct tgcggaagct ggccgacttg gcgctcttga gcaggctctt
     3961 gaccgcgtcg acgccgggct gggcctcggg cggaatctcg gcggcgacgg cgggggccgt
     4021 gacggccagg gcgagaacga aggcgatggt cgcgcgcatg gcgggaaacg ctcctacagg
     4081 gtgacggtct tgacggggtt ggggccgtag cggttggccc cctgttcgcc ggccatcacg
     4141 ccgagctcga cggcggccca gacgatgacg acggaaaagg cgaagtcgag gaaaccggcg
     4201 gggatcggcc agaccacgat cagggccagg atgatgatcg ccgccagcca gcccgaccgg
     4261 ccccggtcgt gcagccgctt ggacagcacg caggcgccgg agaacagcag gcccggatag
     4321 atgaaccagc ccgtcagcag atgcatggtc gggcccatcg aggcctcgta gaccagggcg
     4381 atggcgatca gcaccgccgc ggcgatcagg aacggcgtcc gcgccacgcg gcccgtggag
     4441 gagaagaaca gctccaccca gtcgatctgg ccgctcatgc gttcgctcgc tccgactccg
     4501 ggattgggtc tggaccctat ctaggcgaga gcggcgcgcg gcgaaaggtc ggccgcgggc
     4561 agggggaggc cgggcgcgcg gctgccgtag cggttgggtg cgcgatcacc ctcgccggtc
     4621 accaggctca gcaccagcca cagctgcccg agcacaggcg gcatggcgaa cagcagcatc
     4681 cagcccgaca gcccgcggtc gtgacagcga cgcacggccg ccgccaggct gatccaggcc
     4741 ccgttgagca gcaggaaggc catcagggct gccaccgcgc cctggctggc gccggtgata
     4801 tgggcgccgg actcgtcacc ggcgccggtc agcacgatga tcaggaaggt gaacacatag
     4861 gcggcggtga acacgccgat ctgcgtcagg gcgaagccca ggcgtccggt gcggccgctg
     4921 aatcgataga gatcgtacat ggcgaaaacc ccggcaggtc ggagggttaa cgccgcgacg
     4981 cgacttcggt ttcacggccg atggacccgg cgcgaccgct ctctccctgt tcgatggagg
     5041 atgggccggg acaatggcgt ggccacggtg ggctgacggg cgccggagcc tcatcgcgcc
     5101 gttcgcctcg acccgggcgc ttcccgggaa agtgacgctg acccccgccg gcggaggtca
     5161 gcctcgcgct gtcagccgaa ctcgaccagc acctcgtcgg cggccacgct gtcgccgccc
     5221 ttggcggaaa tggtgatcac cacgccctcg cgttcggcgc ggatgatgtt ctgcatcttc
     5281 atcgcctcga tgacgcagac cacctcgccc tcgcggacgg tctggccgat caccacgtcc
     5341 atgctgacga ccagccccgg catcggcgag atgatcatct tgctggtgtc ggccggctgc
     5401 ttctcgggca gcatgccgtg cagttcggcc gagcgcgggc tcagcaccag caccttggcc
     5461 ctggccgccc ggtggcggat gacgaagccc tcggcagccg gggcgacggt gacggtgaag
     5521 gccctgccgt cgagccgccc gcggaacagc ggcttgcccg gccgccaggc gacgtccgac
     5581 agggtcaggg tgcgaccgcc gcccagcgtc agggtcatca cgccgccggc ctcggcgacc
     5641 gtcacgtcgc ggctgtcatg gccgaccagc acggtccagt cgggacggtc cgatcccccg
     5701 gcgcggcggg tcagcacccg atgcatggcc acgccggcgg ccgtcagcat gtcgacctgc
     5761 gacggcgtgg gcgtcgtgcc gtcgaagccg tcggggaatt cgtcgacgat gtagctggtg
     5821 gccagcttgc cggaccggaa ccgctcctgg tccatcaccg cggcgaggaa cgggatgttg
     5881 tggccaaggc cctcgatgtg gaagtcctcg agcgcccggc ccatgccgtc gatcgccgcc
     5941 agccgggtct cgccccaggt gcagagcttg gagatcatcg ggtcgtagaa catcgagatc
     6001 tcgtcgccct cgcggacgcc ggcgtcgttg cggacgacat aaccgtcatg ttcgccctcg
     6061 accggcgggt cgtagcgcac gaggcggccg atgctgggca ggaacttgcg gtaggggtcc
     6121 tcggcgtaga tccggctctc gatggcccag ccgttgatct tcaggtcctt ctggccgaac
     6181 gccatcgcct cgccggcggc ggatcggatc atctgctcca ccaggtccag gccggtgatc
     6241 agctcggtca ccgggtgttc gacctgcaga cgggtgttca tttccaggaa gtagaagctc
     6301 ttgtcctggc cggcgacgaa ctcgaccgtg ccggcgctgt cgtagttgac ggccttcgcc
     6361 agcgccacgg cctgggcgcc catggccgcg cgggtggcct cgtcgagcag cggggagggg
     6421 gcctcctcga tgaccttctg gttgcgccgc tggatcgagc attcgcgctc gaacaggtgc
     6481 accacgttgc cgtgcttgtc gcccagcacc tggatctcga tgtggcgcgg gctggtgatg
     6541 aacttctcga tgaagatccg gtcgtcgccg aagctcgacc tggcttcggc gcgcacggcc
     6601 gggaagccct cctcgacgtc ctggcggctg tgcgccacgc ggatgccctt gccgccgccg
     6661 ccggcgctgg ccttgatcat caccggatag ccgatctcct cggagatccg gatggcgtgg
     6721 gcggtatcgt cgatctcgcc gatatggccg ggcacgcagc tgacgccggc cgtctcggcg
     6781 aacttcttcg actcgatctt gtcgcccatg gcccggatcg cgccgggatt gggcccgatg
     6841 aacacgatgc cctcgtcggc gcagcgctgg gcgaaggcgg cgttctcgga caggaagccg
     6901 aagccgggat ggatcgcctg ggcgccggtc tcgttgcagg cggcgatgat cttgtcggcg
     6961 acgagatagg actcgctggc cggcgccggg ccgatgaaca cggtctcgtc ggccatctcg
     7021 acggccaggc tgtcggcgtc ggcctcggag tagacgacca ccgtcttgat gcccatgcgc
     7081 cggcaggtct tgatgacccg caccgcgatc tcaccccggt tggcgatcag aattttcgag
     7141 aacatcggct cgcagtcgcc tcttgcgcac gttcgttgcg cgtccggtta gcccgcgatg
     7201 ggcgccattg caacggcgcc gatggggaag gggcgggcgc agggagtggg gagtaggaag
     7261 tagggagtag tagggagtgg caaggggggg gtgcggcgtc ggcggcgtgc gccggtcgaa
     7321 ctactcccta ctccctactc cctacgcgct actccctgac ctcaccccag ccgcaccgcc
     7381 gtcccggtcg ccgtgaccat cagcatgctg ttgttggcgc ccagggactc gtagtcgagg
     7441 tcgaccccca ccaccgcgtc ggcgcccatc gtggcggcct gggcggccat ttcgcgcagg
     7501 gccccctcgc gggcgtcgcg caggacttcc tcgtagctgc cggaccggcc gccgacgatg
     7561 tcgcggatgc cggcgaacag gtcgcggaag atgttggccc cgaggatcac ctcgccggaa
     7621 acgaccccga gggtctcgcg ggtcggccgt tcggcgatgg tcggggtggt ggcggtcagc
     7681 atggacgggt ccctttcagc gatggcgctc ccgccttaca ttggcgctca tgacccgttc
     7741 cgccatatcc gcctccggct tcgacctgac tccgcccacc ggcgccgaac gtgaccggct
     7801 cgaggccgac ctgacgcgtg aggaggccga ggtcctgctg catcacggca ccgaggcgcc
     7861 gttctgcggc gggctgctgg gcgagaagcg gcccggcgcc tactgctgcc gcctctgcgg
     7921 cctgccgctg ttccgcttcg agaccaagtt cgagagcggc acgggctggc ccagcttcta
     7981 cgcgccctgt gacccggccc atgtcggcga ggtgcgcgac acgagctacg gcatgctgcg
     8041 catcgagacc gtctgcgccc gctgcggcag ccaccagggc catgtgtttc ccgacggccc
     8101 gccgccgacc cgcctgcgct actgcatcaa ctcgatctcg ctgaccttcg tcggcgacgg
     8161 cgagcccctg ccggatccgc tcgggcgagg ggattcattc tgatcctgct ccccttgcgg
     8221 gagaaggtgt cggcgcggcc ggcggatggg gggcgatgac cctgtcgggt tgatgaggcg
     8281 acgcgaagcg ggggacatgg cgcgcgccca cttccgacct gcttcgcagg ccaccttctt
     8341 tggcaggggg agaaggagat atgaggactt cccaacttcc ccggcttttg ctaccccccg
     8401 cgcctcacca cgggcggtcg tggcggaatt ggtagacgcg cagcgttgag gtcgctgttc
     8461 cctaaccgga gtggaagttc gagtcttctc gaccgcacca gtgaatcaga ggcaagaagc
     8521 actgcaggat tgatctcttc gttcaggagg ccccgacatg acccgggata aagccgacct
     8581 gatcgaggaa gagaccctgg cggttcccgc cggcgccgaa accgccgccc cgcccgagga
     8641 cctcgaagac ctcgcgttgg gcgacgacta cgccctcaat cccgaatacg tcgacatggt
     8701 catcgacgcc gccg
//
LOCUS       DAORNX010000067         8648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6053_length_8648_cov_6.645642, whole genome shotgun sequence.
ACCESSION   DAORNX010000067 DAORNX010000000
VERSION     DAORNX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_8648_cov_6.645642"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..582)
                     /locus_tag="PLO65_04545"
     CDS             complement(124..582)
                     /locus_tag="PLO65_04545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRD27552.1"
                     /translation="MAESCEVWFYHLERSSLDQVLPDLLDKTLKKGWRAVVRAREAAR
                     LEHLDGWLWSFRDESFLPHGAADEPFAEHQPVLLTTGMDSPNGAQALFLIDGAEPGDL
                     TPHERCIVLFDGRDEAALAGARARWKTFKAEGRPVSYWRQGDQRGWEKMA"
     gene            complement(592..2076)
                     /locus_tag="PLO65_04550"
     CDS             complement(592..2076)
                     /locus_tag="PLO65_04550"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRD27553.1"
                     /translation="MRIEFVAAEAARVLKGGAVAVMAFEGGKLSTAAEDMDKATEGAV
                     TRAIAAGRFAGKPGQTVELLVPAGLDVQRLVVVGSGPDSDWGGDTAERFGAQAFQTVK
                     TSGAETLHVMIPGGKAGEAARAALGATLAAYRFDRYRTTEKPDARPSVKTVKIHTDDV
                     KAAEAAFAPLKALGEAIYFSRDLVSEPANILYPAEFARRVKALESLGLEVEILDEEAM
                     VKLGMGSLLGVGQGSVRDSQLAVIQWKGGGDEQPVAFVGKGVCFDTGGISIKGAEGME
                     DMKWDMGGAAAVAGVMHVLAGRKARVNAVGILGLVENMPDGNAQRPGDVVTSMSGQTI
                     EVINTDAEGRLVLADALWYCQDRFKPKFMVDLATLTGAIIISLGNDYAGLFSNNDALS
                     ENLLAASKAEGEPLWRLPLPDQYNKQIESMIADLKNTGNGRAGGSITAALFLQKFVNG
                     LPWAHLDIASTAWKKPSTVPTIPDGATGFGVRLLNRMVADHYEG"
     gene            2229..3374
                     /gene="lptF"
                     /locus_tag="PLO65_04555"
     CDS             2229..3374
                     /gene="lptF"
                     /locus_tag="PLO65_04555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRD27554.1"
                     /translation="MRLIERYLFRQLLAPTLLAAAALGAVGLLSQSLGQLDIIIEQRQ
                     TAWVFLKVIVLAMPQLLNLTLPIAVFVAALVGLNRLHAEHELVVCYAAGMSRWRVVAP
                     GLQLAVAATIVGLFINLWVQPASYRAMREIFYAAKTDLAATLVREGEFTEPAPRLTVY
                     GQTVESDGLIRNLFIHHRTDKGGATTYTAKEGRITYREGQPVLVMSNGSNQEFSRTGV
                     LNYLSFEEYALDLTPFLNTRGGVTYEASDRYLHELLFPDLSKSYERKNRNKLLAEGHA
                     RLASPLYSITFMMMACAAVLGSSFSRLGYGRRIAVAGAAAAVVRILGFAVQSAAEDSP
                     WLNLMQYLIPVLAGAWAFSQLFRRVERRSGWREWLPFTAGRQRGAAS"
     gene            3371..4511
                     /gene="lptG"
                     /locus_tag="PLO65_04560"
                     /pseudo
     CDS             3371..4511
                     /gene="lptG"
                     /locus_tag="PLO65_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
     gene            4511..6784
                     /gene="lptD"
                     /locus_tag="PLO65_04565"
     CDS             4511..6784
                     /gene="lptD"
                     /locus_tag="PLO65_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRD27555.1"
                     /translation="MDQARRFAGHARLWAGGALMALAAGGTATAQTADVAAPAATAPV
                     ASAEDDGLGERGFYLESDLLIQDDRNQMVTARGGVEIRYRGRTLRAGEVTYDIKSGVI
                     TARDKVVVIDPSGAVTFADVMTLDEELRAGVAEGFSARMANNVKIAAATAVRRSETVN
                     ELNKAIYTPCEICAADPARPPTWSIRADKVVQDRDKQVIYYRNAVIEMFGVPVFYAPV
                     FWHPDPSAPRKSGLLPPKIVGSERRGLSYEQPYAWIISPSEDLVISPQLNTNVNPFVN
                     FQWRRRFQSGQVNARFGYTYEYDLDGDGDRIGDQTSRSYILADGAFDLADHWKWGFAA
                     ERASEDLIFDKYDISDIYAQRGLIPSDDKRLTSQIYAVRQDARSYFSIAAVSIQGLRP
                     GDNDRTFPTIAPLVEGYWEPEGPVFGGRLRFRGRAVGLTREQSQYVATQPGTDSRRAS
                     AGFDWRSSVTFAGGLRLSPFAEARADVFNLSDLPPPNAGQDETITRANGVIGVDVSYP
                     LYRRDGDRTVILEPLLQVALSPDSDPNPLVANEDSSVLEFDETNLLRANKFPGFDLYE
                     GGRRLNVGARATLLYDDGRGVSLLIGRSLRTEPDLQFPARSGLQPKASDWIVAAEGNP
                     IEGLNFFTRARFDASNWDVRRMEFGANLDFDRARGFVRYLRDNQDINGVQREDIDFAG
                     EVMIAGNWGVTFAGVRDVESDVWRRQEFGGLYRDDCLDLALVWVHEETYNRTLGPSDS
                     VILRLTLATLGDKGYRQ"
     gene            6877..8226
                     /locus_tag="PLO65_04570"
     CDS             6877..8226
                     /locus_tag="PLO65_04570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD27556.1"
                     /translation="MAAASVVSLGLAPVAAAQDTPAAAPAEAPAAPAAETPAGPVLAT
                     PETPLPTVSESVAAIVNDDIVSTYDLVQRMRLLVLTAGIQPTQENLPQLQREALRSLI
                     EERLELQELRRVERENKIEIIASDAEVDEAIADMARGANNMTADQLLAQLRAAGVGVE
                     TLRAQLRAEISWQGYISGRFRGRLRIGRDQIKATLDRLAAQASKPQFQISEVYIDAAR
                     VGGMQVAYQGAQSLIDQMRQGAPFAAVARQFSASPTAANGGDAGWISAGEMPAEVDAV
                     LDQLPPGQLSPPIATQDGVYIIYLRNKRAGSNVTMVSLKQIAVPLAEDAPAEQVQAAA
                     ATLEGLRGQVTGCADLEAKAGAVDGVLAGDLGEAELGGLAESFQQAVNTTPDGQLSQP
                     IRTPLGLHLIMVCGRRSGGAQLPTPEQVEARLMGVQLSNISKRILRDLRTSATIETR"
     gene            8223..>8648
                     /locus_tag="PLO65_04575"
     CDS             8223..>8648
                     /locus_tag="PLO65_04575"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRD27557.1"
                     /translation="MTTAPLALSLGDPAGIGPEISVKAWRALRDQGPAFMIIGDALAL
                     ASAPGAPIPIRTVAGPDEAAAVFSDALPVLDLPLSAPVVAGQPSPANGAAVIRWIETG
                     AGLALSGAVSGLVTAPIAKAPLYAAGFRFPGHTEFLADLT"
BASE COUNT         1304 a         3091 c         2901 g         1352 t
ORIGIN      
        1 tgtcggccga ctccggcggg ggcagaggga tggtcggggc ggccgaaatg ggcggcgtcg
       61 acggggccgg cggcgcgccg gcgcaggccg acaaacccag aatcaccaaa agagtcaggc
      121 gtttcaagcc atcttctccc accccctctg gtcgccctgc cgccagtagc tgaccgggcg
      181 cccttccgcc ttgaaggttt tccagcgggc ccgggccccg gccagcgccg cctcgtcacg
      241 gccgtcgaac agcacgatgc agcgttcatg cggcgtgagg tcgcccggct cggcgccgtc
      301 gatcaggaac agggcctggg cgccgttggg gctgtccatg cccgtggtca gcagaacggg
      361 ctgatgctcg gcgaacggct cgtccgccgc gccatgcggc aggaagctct cgtcgcgaaa
      421 gctccacagc cagccgtcga gatgctccag ccgcgccgcc tcccgcgccc gcaccaccgc
      481 gcgccagccc ttcttcaagg tcttgtccag caggtcgggc aacacctggt cgagcgacga
      541 gcgctcgaga tggtagaacc agacttcgca ggactcggcc atgaccgaac cctacccctc
      601 gtagtggtcc gccaccatgc ggttcagcaa gcgcacgcca aagcccgtgg cgccatccgg
      661 gatggtcggc acggtcgagg gcttcttcca ggcggtcgag gcgatgtcga ggtgggccca
      721 gggcaggccg ttgacgaact tctgcaggaa cagcgccgcg gtgatcgagc cgccggcccg
      781 gccgttgccg gtgttcttca ggtcggcgat catgctctcg atctgcttgt tgtactggtc
      841 cggcagcggc agacgccaca gaggctcacc ctcagccttg gacgccgcca gaagattttc
      901 cgatagggcg tcattattgc tgaacaaacc ggcgtagtca ttgccgaggg agatgatgat
      961 cgccccggtc agggtggcca ggtcgaccat gaacttcggc ttgaaccggt cctggcaata
     1021 ccagagggcg tcggccagca cgagacggcc ctcggcgtcg gtgttgatga cctcgatggt
     1081 ctgacccgac atcgaggtga ccacgtcgcc cgggcgctgg gcgttgccgt cgggcatgtt
     1141 ctcgaccagg ccgaggatgc cgaccgcgtt gaccctggcc tttcggcccg caagcacgtg
     1201 catgacgccg gccacggcgg ccgcgccgcc catgtcccac ttcatgtcct ccatgccctc
     1261 ggcgcccttg atgctgatgc cgccggtgtc gaagcagacg cccttgccga cgaaggccac
     1321 gggttgctcg tcgccgccgc ccttccactg gatcaccgcc agctggctgt cgcggacgct
     1381 gccctggccg acacccagga ggctgcccat gcccagcttg accatagcct cctcgtcgag
     1441 gatctcgacc tcgagaccca gcgattccag cgccttgacg cgacgggcga attcggccgg
     1501 atagaggatg ttggccggct cggagaccag gtcgcgcgag aagtagatcg cctcgcccag
     1561 cgccttgagc ggcgcgaagg cggcctcggc ggcctttacg tcgtcggtat ggatcttcac
     1621 cgtcttcacc gatggccggg cgtcgggctt ctcggtggtg cgatagcgat cgaagcggta
     1681 ggcggcgaga gtcgcgccga gggcggcgcg ggccgcttcg ccggccttgc cgccgggaat
     1741 catcacatgc agggtctcgg cgccgctggt cttcaccgtc tggaaggcct gggcgccgaa
     1801 ccgttcggcg gtgtcaccgc cccagtcgct gtccgggccg gatcccacaa ccaccaggcg
     1861 ctgcacatcg agacccgcgg gaaccagcag ttcaacggtc tggcccggct ttccggcgaa
     1921 ccggccggcg gcgatagccc gcgtcaccgc gccctccgtg gccttgtcca tatcctcggc
     1981 ggcggtcgag agcttgcccc cctcgaacgc catcacggcg accgcgccgc ccttgaggac
     2041 cctggccgcc tcggcggcga cgaactcgat gcgcatggat aactcctcag atctgtccgg
     2101 cgggcatagt ccaaccggcg gccataggaa agcttgggcg gttgtatcgc ccttgttcgg
     2161 gcgtatagaa cggacggccc cccgattggg gttttccctg actccagacg ggttcagctt
     2221 cccccactat gcgtctgatc gagcgctatc tgttccgcca gctcctggcg ccgacgcttc
     2281 tcgcggcggc ggccctgggg gctgtcggcc tgctgagcca gagcctgggt cagctggaca
     2341 tcatcatcga gcaacgccag acggcctggg tgttcctgaa ggtcatcgtg ctggccatgc
     2401 cccagctgct gaacctgacc ctgccgatcg ccgtgttcgt cgccgccctg gtcggactca
     2461 accggctgca cgccgagcac gaactggtcg tctgctacgc cgccggcatg agccgttggc
     2521 gggtggtcgc gcctggcctg cagctggcgg tcgcggcgac catcgtgggc ctgttcatca
     2581 acctctgggt ccagccggcc tcgtaccggg ccatgcggga aatcttctac gccgccaaga
     2641 ccgacctggc ggcgaccctc gtgcgtgagg gcgaattcac cgaacccgcg ccgcgcctga
     2701 ccgtctatgg ccagacggtc gaaagcgacg gactgatccg caacctgttc atccaccatc
     2761 ggaccgacaa gggcggggcc acgacctaca ccgccaagga aggccgcatc acctaccgcg
     2821 aaggccagcc ggtgctggtc atgagcaacg gctcgaacca ggagttctcg cgcacgggcg
     2881 tgctgaacta cctgtcgttc gaggaatacg ccctggacct gacgccgttc ctcaacacgc
     2941 ggggcggtgt gacctacgag gcgtcggacc gttacctcca cgagctgctg ttcccggatc
     3001 tctcgaaatc ctacgagcgg aaaaaccgca acaagctgct ggccgagggg cacgcccggc
     3061 tggcctcgcc gctctacagc atcaccttca tgatgatggc ctgcgccgcc gtgctggggt
     3121 cgagtttcag ccggctgggc tatggccgcc gcatcgccgt cgcgggggcc gccgccgccg
     3181 tggtgcggat tctcggtttc gccgtgcagt cggccgccga ggacagcccg tggctgaacc
     3241 tgatgcagta cctgatccct gtgctggccg gcgcctgggc gttcagccag ctgttccggc
     3301 gcgtcgagcg gcgcagcggc tggcgggaat ggctgccctt cacggccggc cgccagcgcg
     3361 gagcggcgtc atgatccgcg gaatcggccg gcttgagcgc tatgtgctga cacgaaccct
     3421 ggcgggcgtc gccggcgcgc tggcggtgat cggcgcggtg atcctgctga tcgatttcgt
     3481 cgaggtctcg cgcaccgtcg gtggtcgggg cgacgcaggc tttccgcgcc tgctgtggct
     3541 gaccatgctc aaggccccgt cgacgattct gaccctgctg ccgttcgcct tcctgttcgg
     3601 gaccctggcc gccttcgtcg ggctcaaccg gcgcagcgag ctgatcgcca tgcgcgcggc
     3661 cggcgtctcg gcctggcggt tcatcctgcc ggccgccgtg gccgccttcg tcgtgggcgc
     3721 ggcgacggtc tcggcgctga atccgctggc cgcgatgctg ggggcctcgt tcgagcggtc
     3781 acgcaccgcg atcgccgagg aattccagac ctcggccgcc aaggacacct ggctgaggca
     3841 gggcgacgcc acctcccagg tggtgatccg cgcccggtcg catgacgagg tcaacgggac
     3901 ggtgcggctc aaggacgtgt cgatgttcat ctaccgcgtc acgcccctgg gcggcctgga
     3961 gttctcccgc cggctcgagg cggaggaagc ccggttgatg cccggatact ggcgcctgcg
     4021 cggcgtccgc gaggccgcgc ccggcgccgg ctcggtgcgg tccgagaccc tgtcgatccc
     4081 ctcgacgctc gacgagcagg cggcgatcga gcgattcacg acgcccgacg cgatcgcctt
     4141 ctggcggctg ccggcgacga ttcgcggcac ggaggcggcc gggttctcgg ccctgcccta
     4201 tcgcctgagg ctgcaccagc tgctggccac gccgctgctc tacgcggccc tgtcggtgct
     4261 ggccgcggcc ttctcgctgc ggctgatgcg gctggggggc ctggccgccc tggcggcgtc
     4321 gggcgtggcc ctcgggttca tcttcttttt cttcaaccag ttctgcggcg cgctcggcaa
     4381 ggccgagatc gtgccggcct tcgcggccgc ctgggcgccg ccgctgcttg cgctgttatc
     4441 ggacgctgct ttgctatacg gaggacgggg gaatcatcgg gggccgcgcc gccgggagaa
     4501 agagcgcctg atggaccaag ctcgtcgctt cgccggacat gcccgcctgt gggccggcgg
     4561 cgccctgatg gcgctggccg cggggggaac cgccaccgcc cagacggcgg acgtcgccgc
     4621 cccggccgcg accgccccgg tcgcgtccgc cgaggacgac ggcctgggcg aacgcggatt
     4681 ctacctggaa tccgacctgc tcatccagga cgaccggaac cagatggtca cagcccgcgg
     4741 cggggtcgag atccgctatc gcggccgcac gctgcgggcc ggcgaggtga cctacgacat
     4801 caagagcggg gtcatcaccg cccgcgacaa ggtggtcgtc atcgacccgt caggggcggt
     4861 gacgttcgcc gacgtgatga cgctcgacga ggagctgcgc gccggggtcg ccgagggctt
     4921 ctcggcccgc atggccaaca atgtgaagat cgccgccgct accgccgtcc ggcgcagcga
     4981 gacggtcaac gaactcaaca aggcgatcta caccccgtgc gagatctgcg ccgccgaccc
     5041 ggcccggcca cccacctggt cgatccgcgc cgacaaggtg gtccaggatc gcgacaaaca
     5101 ggtcatctac taccgcaacg ccgtgatcga gatgttcggc gtgccggtgt tctacgcccc
     5161 ggtgttctgg catcccgatc ccagcgcccc gcgcaagtcg ggcctgctgc cgcccaagat
     5221 cgtcggctcc gagcgccggg gcctgtccta tgaacagccc tacgcctgga tcatctcgcc
     5281 gtccgaagac ctggtgatca gcccgcaact gaacaccaac gtcaatccgt tcgtgaactt
     5341 ccagtggcgt cgccgcttcc agtccggcca ggtcaacgcc cgtttcggct atacctacga
     5401 atacgacctg gatggcgacg gcgaccggat cggcgaccag acctcgcgca gctacatcct
     5461 cgccgatggc gcgttcgacc tggccgacca ctggaaatgg gggttcgccg cggaacgggc
     5521 gtctgaagac ctgatcttcg acaagtacga catcagcgac atctacgcgc agcgcgggct
     5581 gatcccctcc gatgacaagc gcctgacctc gcagatctac gccgtgcggc aggacgcgcg
     5641 gtcctatttc agcatcgccg ccgtcagcat ccagggcctg cggcccggcg acaatgaccg
     5701 gacctttccg accatcgccc cccttgtcga gggctactgg gagccggagg ggccggtgtt
     5761 cggcggacgg ctgcgcttcc gcggccgggc ggtcggcctg acccgcgagc agtcgcagta
     5821 cgtggccacc cagccgggaa ccgacagtcg ccgggcgtcg gccggcttcg actggcgcag
     5881 cagcgtgacc ttcgccgggg ggctgcggct gtcgccgttc gccgaagcca gggccgacgt
     5941 cttcaatctc agcgacctgc cgccaccgaa cgcgggccag gacgagacga tcacccgcgc
     6001 caacggcgtg atcggggtgg acgtcagcta tccgctctat cgccgcgacg gcgaccgaac
     6061 ggtcatcctc gagccgctgc tgcaggtcgc cctgtcaccc gacagcgatc cgaacccgct
     6121 ggtggccaac gaagacagtt cggtgctcga gttcgacgag accaacctgc tgcgggcgaa
     6181 caagtttccc ggcttcgatc tctacgaggg cggacggcga ctcaacgtcg gcgcccgcgc
     6241 caccctcctc tacgacgacg gccggggtgt cagcctgctg atcggccgca gcctgcgcac
     6301 cgagcccgac cttcagttcc cggcccgcag cggcctgcag cccaaggcct cggactggat
     6361 cgtcgccgcc gagggcaatc ccatcgaggg cctgaacttc ttcacccgcg cccgtttcga
     6421 cgcttccaac tgggacgtgc ggcggatgga gttcggggcg aacctggact tcgaccgcgc
     6481 ccgcggcttc gtccgctacc tgcgggacaa ccaggacatc aacggcgttc agcgcgagga
     6541 tatcgacttc gccggcgagg tgatgatcgc cggcaactgg ggcgtgacct tcgccggggt
     6601 ccgcgacgtg gagagcgacg tctggcgccg gcaggaattc ggcggcctgt accgggacga
     6661 ctgcctcgac ctggcgctgg tctgggtgca cgaggaaacc tataaccgga cgctcggccc
     6721 gtcggactcg gtgatcctgc gcctaaccct cgccacattg ggcgataaag gctacaggca
     6781 atgacgaacg gccggaaatc cgcgtttccg ccgggccaaa ctctcaaggg aacgaccaga
     6841 ttgatgacgt cggcgcgtag tttgacgggc ttggccatgg cggccgcgtc cgtcgtaagc
     6901 cttggcctcg cccccgtggc ggccgcccag gacacgcccg ccgcggctcc ggccgaagcg
     6961 cccgcggcgc ccgcggccga gacgcccgct ggtccggtcc tggccacccc cgaaacgccg
     7021 ctgccgacgg tctccgaaag cgtcgccgcg atcgtcaatg acgacatcgt ctcgacctac
     7081 gatctggtcc agcgcatgcg cctgctggtg ctgaccgcgg gcattcagcc gacccaggag
     7141 aacctgcccc agctgcagcg cgaggctctg cgcagcctga tcgaggaacg gctggagctg
     7201 caggagctgc ggcgggtcga acgcgagaac aagatcgaga tcatcgcctc cgacgcggaa
     7261 gtcgatgagg cgatcgccga catggcccgc ggggccaaca acatgaccgc cgaccagctg
     7321 ctcgcgcagt tgcgggccgc cggggtcggg gtcgagaccc tgcgcgccca gctgcgggcc
     7381 gagatcagct ggcagggcta tatcagcggg cggttccggg gccggctgcg gatcggccgc
     7441 gaccagatca aggcgacgct cgaccggctg gccgcccagg ccagcaagcc gcagttccag
     7501 atcagcgaag tctacatcga cgccgcccgc gtcggcggca tgcaggtggc ctaccagggc
     7561 gcccagtcgc tgatcgatca gatgcggcag ggcgcgccgt tcgccgccgt ggcccgccag
     7621 ttctccgcct cgccgaccgc cgccaacggc ggggacgcgg gctggatcag cgccggcgag
     7681 atgccggccg aagtcgacgc ggtgctcgac cagctgccac cgggtcaact gtcgccgccg
     7741 atcgcgaccc aggacggggt ctatatcatc tacctgcgca acaagcgcgc cgggtcgaac
     7801 gtgacgatgg tcagcctcaa gcagatcgcc gtgcccctgg ccgaggacgc gcccgccgaa
     7861 caggtgcagg ccgccgcagc gacccttgaa ggcctgcgtg gccaggtgac cggctgcgcc
     7921 gacctcgaag ccaaggccgg cgccgtggac ggcgtgctcg ccggcgacct gggcgaggcc
     7981 gaactcggcg gcctggccga gtccttccag caggccgtca acaccacgcc cgacggtcag
     8041 ctgtcgcagc cgattcgcac gccgctcggc ctgcacctga tcatggtctg cggtcgccgc
     8101 tccggcggcg cccagctgcc gacccccgaa caggtcgagg cgcggttgat gggcgtccag
     8161 ctgtccaata tctccaagcg cattctgcgc gacctgcgca caagcgcgac catcgagacg
     8221 cggtgacgac ggcgccactg gccctgagtc tgggcgatcc cgccgggatc ggcccggaga
     8281 tttcggtcaa ggcctggcga gcgcttcgcg accagggacc agccttcatg atcatcggcg
     8341 acgccctggc cctggcctcg gcgcccggcg cgccgatccc gatccgcacc gtggccggcc
     8401 ccgacgaggc tgccgccgtc ttctctgacg ccctgcctgt gctcgacctg cccctgtcgg
     8461 cccccgtggt cgccggccaa cccagtccgg ccaacggcgc cgcggtcatt cgctggatcg
     8521 agaccggggc gggcctggcc ctgtccggcg cggtgagcgg cctggtcacg gctccgatcg
     8581 ccaaggcccc gctgtacgcc gccggcttcc gctttcccgg ccacaccgag tttctcgccg
     8641 acctgaca
//
LOCUS       DAORNX010000068         8608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6094_length_8608_cov_7.325967, whole genome shotgun sequence.
ACCESSION   DAORNX010000068 DAORNX010000000
VERSION     DAORNX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_8608_cov_7.325967"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLO65_04580"
     CDS             <1..228
                     /locus_tag="PLO65_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27558.1"
                     /translation="NIVVTAIAPGAFASDMNITARDQSEAVAARIPSRRIGTDEDMQG
                     AAIYLASRAGDYCVGITLAVDGGVAYAAAAI"
     gene            249..785
                     /locus_tag="PLO65_04585"
     CDS             249..785
                     /locus_tag="PLO65_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27559.1"
                     /translation="MFNTALIRTRLGQYGAAWLAGYVLALVAVLAGAWLTPTPLVRVA
                     DLVLPVALAALTLAVLAALVATLTAGRETVATRLVIVLLALMLFLPLLWAPVLGAITA
                     AWIGHVSIEYSAVYAQFRILVSQALFRLAGLVFANPLLDTVWSLFQGLATVVGFFSAF
                     FQVWPRVLRLLGAREAEA"
     gene            885..1514
                     /locus_tag="PLO65_04590"
     CDS             885..1514
                     /locus_tag="PLO65_04590"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRD27560.1"
                     /translation="MLASLLLRIGALGPLIFYSVIVAGGFMSPGYNHMTQFASELGQA
                     GSPSAQLFNYGIMAVGGAMIVGGLGFLLGMRKLGGGVLMPALAAVAIALYGVSFIIGG
                     LHPMPDPMHEAYKLVYAGIAAPLLAFLAMGDRTELSGPKTLAVICFLASAVLLALMMN
                     VGGFNLVHKTDVGLWQRGLIVATTLWMLAAFLAIPGALAAKERKRTSAY"
     gene            complement(1527..2147)
                     /locus_tag="PLO65_04595"
     CDS             complement(1527..2147)
                     /locus_tag="PLO65_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HRD27561.1"
                     /translation="MTSRTLFATRLFEASLAGGKGFSALNADLEDACRMLAAEDRAGR
                     AWCRDNGYPGYTSYASLDDLPTRVTAFGDLKRRLDRQAAAFAAEVAFDLKGRRLVMDS
                     LWVNILRPGGTHTSHIHPHSVISGTYYVATPPGASALKLEDPRLPMMMAAPGRTKDAP
                     EDLQTFVYVTPAPGTVLMWESWLRHEVPANGARSDRISVSFNYAWR"
     gene            complement(2293..3888)
                     /locus_tag="PLO65_04600"
     CDS             complement(2293..3888)
                     /locus_tag="PLO65_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HRD27562.1"
                     /translation="MAERFDISRRALVGLTLSAAASPALAQVVSPAITAGFQGLDAGK
                     GVTAYKGIPYARPPVGDLRFAPPQRLPPPAGRQTWTRFGPPAIQNPSPSSVHPTSELG
                     KALQAIFPTAAEVAAQSEDCLYLNVWTTARGPETGRPVMVWLHGGGFAYGSGAWPIYD
                     GTNLAAEGAVIVTLNHRLNVFGYADLPGVPGSGNAGMLDIVAALEWVRDNIYLFGGDP
                     CNVTIFGESGGGAKVSYLLAMPRARGLFHRAIIQSGPGVKAVPKDRAAALTADLYAEL
                     GIRPGDIGALRAVPAERLVAAAAAAEAKQPGAGFDRGGFSPVADGDILPIQPWDPAAP
                     ATAHDVPVMIGINKDEMTLFMASAPWFGRLDQAGLEAMAGGMFGAKAPTVLAALKRDF
                     PNDSPTYLATHLVTYGRMFAGSVQIAERKAAQGGAKAYMYLLEWETPVGPFRSPHTLE
                     IPLVFDNVDRARVLVGEGPAPQVLAKQMSAAWLAFARTGHPNTPVIPWWPAYDAETRS
                     VMVFNLQSRVVNDPYAATRRAVT"
     gene            4034..5107
                     /locus_tag="PLO65_04605"
     CDS             4034..5107
                     /locus_tag="PLO65_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HRD27563.1"
                     /translation="MKAAVLREVGKPLQIEDVTINKPGPHEVLIRTKAAGVCHSDLHF
                     VEGSYPHPLPAVLGHESAGVVEAVGSEVRTVKAGDHVITCLSAFCGHCNHCVTGHMAR
                     CVSGDVRRAKDDEPRLGNGLNQFLNLSSFAEQMLIHEHACVAIRKDMPFDRAALIGCS
                     VTTGVGAVVQTSSVRAGETVAVIGCGGVGLAAVNGAAIAGAGRIIAIDMHASKLNLAK
                     HFGATDVIDASQDDPVKAVMELTKGGVDHSFEAIGLKKTTEQAFKMLARGGTCNVIGM
                     IPVGTMIELHGADFLGEKRIQGSLMGSNRFPVDMPRLVDFYMAGKLKLDEMIGQRIKL
                     ADVNQAFEELKRGELARSVIVFD"
     gene            complement(5201..5638)
                     /locus_tag="PLO65_04610"
     CDS             complement(5201..5638)
                     /locus_tag="PLO65_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27564.1"
                     /translation="MGLAGEEIEVADVDEVALAARLAGEFFVRGVEIVARAHGGDLLR
                     GIIFTAIAVANSEAAPMTGGGARRPVSVMSIAHSIGVPYETTRRYVNMLVADGLCVRE
                     GRRGVIVPEQALLRPELEAAYRETFVSFNRLATALRRMSYAPA"
     gene            5949..6233
                     /locus_tag="PLO65_04615"
     CDS             5949..6233
                     /locus_tag="PLO65_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27565.1"
                     /translation="MAEIVEVLLRLGGTAHRDMVAQQVALARGGGGAPPPPALKARLE
                     AAFTTRLRLDRSAPDGAALFALPFGEESHRWALAREAEIFLRQEHRPGGR"
     assembly_gap    6309..6318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6330..6815)
                     /locus_tag="PLO65_04620"
     CDS             complement(6330..6815)
                     /locus_tag="PLO65_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27566.1"
                     /translation="MPTRTEKRRDRLALRASTTFLLNSVDLMSRVVGGDILRGVIFTA
                     IVDANVRHIRPGDVVGQTYSEAGDQVPDELRRPISVHALALELDIPYETTRRHVNALI
                     ADGFCQRTETGIVVPGSVIARDQMAMALRRNFENLRRLIGDLREGGVEFAIHGETLPG
                     R"
     gene            complement(6948..8090)
                     /gene="ispG"
                     /locus_tag="PLO65_04625"
                     /gene_synonym="gcpE"
     CDS             complement(6948..8090)
                     /gene="ispG"
                     /locus_tag="PLO65_04625"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRD27567.1"
                     /translation="MSHDDHTHLRPWRHIDRRKSRQIHVGRVPVGGDAPITVQSMTNT
                     LTADAAATIDQIRQLEEAGADIVRVSCPDVESTAALPAIVKAAKVPIVADIHFHYKRG
                     IEAAKAGAACLRINPGNIGSPDRVREVIQAARDHGCSMRIGVNAGSLERDLLEKYGEP
                     CPDAMVESALSHARILQDHDFHEFKISVKASDIFMTVAAYQQLADAIDCPLHLGITEA
                     GAQRTGTVKSAIGMGNLLWAGIGDTIRVSLAADPVEEIKVGFDLLKSLGLRHRGVNII
                     ACPSCARQGFNVIKTVEALEARLAHIATPLSLSIIGCVVNGPGEALMTDLGFTGGGKG
                     SGMVYVAGRPDHKMDNDRMVDHIVELVEARAALIEAEATSGLQAAE"
     gene            complement(8167..>8608)
                     /locus_tag="PLO65_04630"
     CDS             complement(8167..>8608)
                     /locus_tag="PLO65_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27568.1"
                     /translation="QFGSMLGAEPDPGLEASAAADGSADAVTAAAKIRKEAEMAAAAS
                     AAAEAQVSPGTPFVAKGPVHGADANASVVTFQARKATTLIVRGADGSVYFARALAAGE
                     AYRAPNIKGVAVEVGDPAAIDVFVGGRLKGAMSATTASVSSLAG"
BASE COUNT         1263 a         2922 c         3079 g         1334 t
ORIGIN      
        1 aacatcgtcg tcaccgccat cgcgccgggg gccttcgcct cggacatgaa catcaccgcc
       61 cgggaccagt ccgaggccgt cgcggcccgt ataccctcac gccgcatcgg cacggacgag
      121 gacatgcagg gggcagccat ctacctcgcc tcgcgggccg gcgactactg tgtcgggatc
      181 accctggcgg tcgacggcgg cgtggcctac gccgccgcgg cgatctgagg gaaggggagg
      241 ggccggccat gttcaacacg gcgctgatcc gaacccggct gggccagtat ggcgcggctt
      301 ggctcgcggg ctacgtcctg gccctggtgg ccgtgctggc gggggcctgg ctgacaccca
      361 ccccgctggt ccgggtggcc gatctggtgc tgccggtggc gctggcggcg ctgaccctgg
      421 cggtgctggc cgcgctggtc gccacgctga ccgccgggcg cgagaccgtg gcgacccggc
      481 tggtcatcgt gctgctggcc ctgatgctgt ttctgccact gctgtgggcc ccggtgctgg
      541 gcgccatcac ggcggcctgg atcggccatg tctcgatcga gtactcggcc gtctacgccc
      601 agttccgcat cctggtcagc caggccctgt tccgcctcgc cggcctggtg ttcgccaatc
      661 cgctgctgga tacggtctgg tccctgttcc agggactggc gacagtggtc ggctttttct
      721 cggccttctt ccaggtctgg ccccgcgtcc tgcggctgct gggcgcccgg gaggcagagg
      781 cctagagggg aagcccgctc acctgcaacg ggcatggcgt tgacgccgcc gcgagggact
      841 gcgagcgtcg aggccgggaa aaaccaataa gggggaggcc aggaatgctc gcctcgttgc
      901 tgctgcgaat cggggccctt ggcccgctca tcttctacag cgtgatcgtg gcgggcggct
      961 tcatgtcgcc gggctacaac cacatgaccc agttcgccag cgaactgggc caggccggtt
     1021 cgccgtcggc gcagctgttc aactacggca tcatggccgt cggtggcgcg atgatcgtcg
     1081 gcgggctggg cttcctgctc ggcatgcgca agctgggcgg cggcgtgctg atgccggccc
     1141 tggcggccgt cgccatcgcg ctctacggcg tctcgttcat catcggcggc ctgcatccga
     1201 tgccggaccc gatgcacgag gcctacaagc tggtctacgc cggcatcgcc gcgccgttgc
     1261 tggccttcct ggccatgggc gaccgcacgg aactgtccgg ccccaagacc ctggccgtga
     1321 tctgcttcct cgccagcgcc gtgctgctgg cgctgatgat gaacgtcggc ggcttcaacc
     1381 tggtgcacaa gaccgacgtc ggactgtggc agcgggggct gatcgtggcc acgaccctgt
     1441 ggatgctcgc cgccttcctc gcgatccccg gcgccctggc ggccaaggaa cgcaagcgga
     1501 cttccgccta ctagggcgac cgccggctag cgccaggcgt agttgaaact gacggagatg
     1561 cggtcgcttc tggcgccgtt ggccggcacc tcatgcctga gccagctctc ccacatcagc
     1621 acggttcccg gcgccggagt gacgtagacg aaggtctgca ggtcctccgg cgcatccttc
     1681 gtgcggccgg gcgcggccat catcatcggc aggcggggat cctcgagttt cagcgccgag
     1741 gctccggggg gcgtggcgac gtagtaggtc ccgctgatca cgctgtgcgg gtggatgtgg
     1801 ctggtgtggg tcccgcccgg cctgaggatg ttgacccaca ggctgtccat caccagccgt
     1861 cggcctttca ggtcgaacgc cacctccgcc gcgaaggcgg ccgcctgcct gtcgagcctg
     1921 cgcttcaggt cgccgaaggc cgtgacccgc gtcggcaggt cgtcgagcga ggcgtaggag
     1981 gtgtagcccg gatagccgtt gtccctgcac caggcgcgcc cggcccggtc ctcggccgcc
     2041 agcatgcggc aggcgtcctc gaggtcggcg ttgagggcgg aaaagccctt gccgccggcc
     2101 aggctggcct cgaacaggcg ggtcgcgaaa agggtgcggc tggtcatggc ttggcgttag
     2161 gccgcggaag ggcaggtgtc aaagcgggcc gtgcggcctt cgacacgcat cctggggtgc
     2221 tgctcaggat gacgtgttcc tgctccacgt catggtgagc agcgctcgaa gagcccgtgg
     2281 cgaaccacgc aatcaggtca ccgccctgcg cgtggccgca taggggtcgt tgaccacgcg
     2341 gctctgcagg ttgaacacca tcacagagcg cgtctccgcg tcgtaggccg gccaccaggg
     2401 gatgaccggg gtattggggt ggccggtgcg ggcgaaggcc agccaggcgg cgctcatctg
     2461 cttggccagc acctgcgggg ccgggccctc cccgaccagc acccgggccc ggtcgacatt
     2521 gtcgaacacc agcgggattt ccagcgtgtg gggcgagcgg aaggggccga cgggcgtctc
     2581 ccactccagc aggtacatat aggccttggc cccgccctgg gcggccttgc gctcggcgat
     2641 ctgcaccgag ccggcgaaca tccggccgta ggtcaccaga tgcgtcgcca ggtaggtcgg
     2701 gctgtcgttc ggaaaatcgc gtttcagcgc cgccagcacc gtcggcgcct ttgcgccgaa
     2761 catgccgccg gccatggcct cgaggccggc ctggtcgagc cggccgaacc agggcgcgga
     2821 ggccatgaac agcgtcatct catccttgtt gatgccgatc atcaccggaa cgtcgtgcgc
     2881 cgtcgcgggc gccgccgggt cccacggctg gatcggcagg atgtcgccgt cggcgaccgg
     2941 cgagaagcca ccgcggtcga agcccgcgcc tggctgtttc gcctcggccg ccgccgccgc
     3001 ggccaccagc cgttccgccg gcacggcgcg cagcgcgccg atgtcgcccg gcctgatgcc
     3061 cagttcggcg tagagatcgg cggtcagcgc cgccgcgcgg tccttcggca cggccttgac
     3121 gccggggccg ctctggatga tcgcccggtg gaacagacct ctcgcccggg gcatggccag
     3181 caggtagctg accttcgcgc caccgccgga ctcgccgaag atggtcacat tgcagggatc
     3241 gccgccgaac agatagatgt tgtcgcgcac ccattcgagc gccgcgacga tgtcgagcat
     3301 cccggcgttg cccgaaccgg ggacgccggg aaggtcggca tagccgaaca cgttcaggcg
     3361 gtggttgagg gtgacgatca ccgcgccctc ggccgccagg ttggtcccgt catagatggg
     3421 ccaggcgccg gagccgtagg cgaaaccgcc gccgtgcagc cagaccatca ccggccggcc
     3481 ggtctccggg ccgcgggccg tggtccagac attgagatag aggcagtcct cgctctgggc
     3541 ggcgacctcg gccgcggtcg ggaagatggc ctgcagcgcc ttgccgagct cggacgtggg
     3601 atgaaccgac gagggcgacg ggttctggat cgccggtggt ccgaaccggg tccaggtctg
     3661 tcgcccggcc ggcgggggca ggcgctgcgg cggcgcgaac cgcagatcgc cgaccggcgg
     3721 acgggcgtag ggaatgccct tgtaggcggt gacgcccttg ccggcgtcga ggccctggaa
     3781 acccgcggtg atggccggag aaacaacctg ggccagggcg gggctcgccg cggccgacag
     3841 ggtcaggccg accagggcgc gtcgggagat gtcaaagcgt tcggccatgt ttcgtctcca
     3901 cctaccagga acatcggtat cggatgcggc cgcgcccgtc gagattcttc ccgccttacg
     3961 gtgcggcagg ccttccgctc gcagggtgaa cggcgataac gtcaggccag ccaattcaag
     4021 gggagagacg tcgatgaagg ccgcagtgct tcgcgaagtg ggcaagccgc tccagatcga
     4081 ggacgtgacg atcaacaagc cgggcccgca cgaggtgctg atccgcacca aggccgccgg
     4141 cgtctgtcac tcggacctgc acttcgtcga agggtcctat ccgcacccgc tgcccgccgt
     4201 gctgggccac gagagcgccg gcgtcgtcga ggcggtcggc agcgaggtcc gcacggtcaa
     4261 ggccggcgac catgtgatca cctgcctgtc ggccttctgc ggccactgca accactgcgt
     4321 cacaggccac atggcccgct gcgtcagcgg tgacgtgcgc cgggcgaagg acgacgaacc
     4381 gcgtctgggc aatggtctca accagttcct caacctgtcg tcgttcgccg agcagatgct
     4441 gatccacgag cacgcctgtg tcgcgatccg caaggacatg ccgttcgacc gggcggccct
     4501 gatcggctgc tcggtgacca cgggcgtcgg cgcggtggtg cagacctcca gcgtccgggc
     4561 cggcgagacg gtggccgtca tcggctgcgg cggcgtgggt ctggcggcgg tcaacggcgc
     4621 ggcgatcgcc ggcgccggcc ggatcatcgc catcgacatg cacgcctcca agctgaacct
     4681 ggccaagcac ttcggcgcca cggacgtgat cgacgcgagc caggacgacc cggtcaaggc
     4741 ggtgatggag ctgaccaagg gcggcgtcga ccacagcttc gaggccatcg gcctgaagaa
     4801 gaccaccgag caggcgttca agatgctggc ccgcggcggc acctgcaacg tcatcggcat
     4861 gatcccggtc ggcacgatga tcgagctgca cggcgccgac ttcctcggcg agaagcgcat
     4921 ccagggctcg ctgatggggt ccaaccggtt cccggtcgac atgccccgcc tggtcgactt
     4981 ctacatggcc ggcaagttga agctcgacga gatgatcggc cagcggatca agctggcgga
     5041 cgtcaaccag gccttcgagg agctcaagcg cggcgaacta gcccgttcgg tcatcgtctt
     5101 cgactagggc cgccggcctc cggttcgtga gacatggccg ggcccggttg gtcccgcgac
     5161 ggcggggacg gccgggcttc cgccgacggg ccgaccgccc ctaggcgggc gcgtagctca
     5221 tgcgccgcag ggccgtggcc agccggttga agctgacgaa ggtctcgcgg taggcggcct
     5281 ccagttcggg acgcagcagc gcctgttcgg ggacgatcac cccgcgccgg ccttcgcgca
     5341 cgcacaggcc gtcggcgacc agcatgttca cgtaccggcg ggtggtctcg tagggcacgc
     5401 cgatcgaatg ggcgatcgac atgacgctga ccggccggcg cgcgccgccg ccggtcattg
     5461 gcgcggcctc gctgttggcc accgcgatgg cggtgaagat gatcccccgc aacagatcgc
     5521 cgccgtgggc gcgggcaacg atctcgacgc cccggacgaa gaattccccg gccagccggg
     5581 ccgccagggc gacctcgtcg acatcggcga cctcgatctc ttcccctgcc aagcccaatc
     5641 aaccctcccc ccggcggcga ccgatccggg ccggctcgcg ccggcgacgc ccatactccc
     5701 caaaatgagt atacatcccg tcaccgcata cggccgtcgg cctatcgtcg gcaggctgac
     5761 ggagggggga agtcgacatg gcggagcgct ggcaacgtac ggccccgcat gatcgcgcgc
     5821 ggcggtcgcc gggctgcggg atgagtcccg atccctcagg gaggcgaaca ggcgaatcgg
     5881 ccgtcattct cgattacatg gaccggcgtc gccgaaaggc aagcgcgcag gccggcgacg
     5941 acggactggt ggccgagatc gtcgaggttc tgctgcggct gggcggcacg gcgcacaggg
     6001 acatggtcgc ccagcaggtg gcgctggccc gaggcggcgg cggcgcgccc ccgcctccgg
     6061 cgttgaaggc ccggctcgag gcggcgttca cgacccgatt gcgcctggac cggtcggcgc
     6121 cggacggggc cgccctgttc gccctgccgt tcggcgagga gtcgcaccgc tgggccctgg
     6181 cgcgggaggc ggagatcttc ctgcgtcagg aacaccggcc cggcgggcga taggacacac
     6241 gccggacgcg ccgtccggcg tacgacgacg gcggccgcct gccccccccc gatatggccg
     6301 ccggcgggnn nnnnnnnntc tccggtcgct caccggccgg ggagcgtttc gccgtggatc
     6361 gcgaactcga cgccgccttc gcgcagatcg ccgatcagcc ggcgcaggtt ctcgaaattg
     6421 cggcgcagcg ccatggccat ctggtcgcgg gcgatgactg atccggggac cacgatgccg
     6481 gtctcggttc gctgacagaa gccatcggcg atcagggcgt tcacatggcg acgggtcgtt
     6541 tcgtagggaa tgtcgagttc cagggcgagg gcgtgcacgc tgatcggccg gcgcagctcg
     6601 tcgggcacct ggtcgccggc ctccgaatag gtctgcccga ccacgtcgcc cgggcggatg
     6661 tggcgcacgt tggcgtcgac gatggcggtg aagatcaccc cgcgcaggat atcgccgccc
     6721 acgacccggg acatcaggtc gacggagttc agcaggaacg tcgtcgaggc gcgcagcgca
     6781 aggcggtcac ggcgtttttc tgtccgagtg ggcacgaagc gtctcctgtc ccggtccggg
     6841 tgactgggga gtaaccagaa ccaccgtttc tggacgcaat gtgctgatct ttcaaacgtc
     6901 agcctccccg atttgaggaa aggcgtctgc ggccagacgt cgcggcgcta ctcggcggcc
     6961 tgcaggccgg aggtcgcttc cgcctcgatc agggcagccc gggcctcgac cagctcgacg
     7021 atgtggtcga ccatccgatc gttgtccatc ttgtggtccg ggcgaccggc cacatagacc
     7081 atgcccgagc ccttgccgcc gcccgtgaag cccaggtcgg tcatcagcgc ctcgccggga
     7141 ccgttgacca cgcagccgat gatcgacagc gacagcggcg tggcgatatg ggccagccgc
     7201 gcctcgaggg cctcgaccgt cttgattacg ttgaagccct gacgggcgca ggaggggcag
     7261 gcgatgatgt tcacgccccg gtggcgcagg cccagggact tgagcagatc gaagccgacc
     7321 ttgatctcct ccaccggatc ggcggccagg ctgacgcgaa tggtgtcgcc gatgccggcc
     7381 cacagcagat tgcccatgcc gatggccgac ttgaccgtgc ccgtgcgctg cgcgcccgcc
     7441 tcggtgatcc cgaggtgcag cggacagtcg atggcgtcgg ccagctgctg gtaggcggcc
     7501 acggtcatga agatgtccga ggccttcacg ctgatcttga actcgtggaa gtcgtggtcc
     7561 tgcaggatgc gggcgtggct gagcgcgctt tccaccatcg cgtcggggca gggctcgccg
     7621 tacttttcga gcaggtcccg ctccagcgag ccggcgttga ccccgatgcg catggagcag
     7681 ccgtggtcgc gggcggcctg gatcacctcg cgcacgcggt cagggctgcc gatgttgccg
     7741 ggattgatac gcaggcaggc cgcgccggcc ttggccgcct cgatgccgcg tttgtagtgg
     7801 aagtggatgt cggcgacgat cgggaccttg gcggccttga cgatcgccgg cagggcggcg
     7861 gtcgactcca cgtcgggaca ggagacgcgg acgatgtcgg cgccggcctc ctccagctgg
     7921 cggatctggt cgatggttgc cgccgcgtcg gcggtcaggg tgttggtcat cgactggacg
     7981 gtgatcgggg cgtcgccgcc gaccggaacc cgcccgacgt gaatctgccg ggacttgcgc
     8041 cggtcgatat ggcgccaggg ccgcaggtgg gtgtgatcgt cgtggctcat gagacgtcca
     8101 aggtaggcgc gtgcgcgagg tttcccaacc ccgtcatcga aacttcgcgt cgatgatccg
     8161 acgggctcag ccggccaggg acgagaccga ggcggtggtc gcgctcatgg cccccttgag
     8221 cctgccgccg acgaagacat cgatcgccgc ggggtcgccg acctcgacgg ccacgccctt
     8281 gatgttcggc gcgcggtagg cctcgccggc cgccagggcg cgggcgaaat agaccgagcc
     8341 gtccgcgccg cgcacgatca gggtcgtggc cttgcgggcc tggaaggtga cgaccgaggc
     8401 gttggcgtcg gcgccatgaa cggggccctt ggccacgaag ggcgtgcctg gcgagacctg
     8461 cgcctcggcc gcggcgctcg cggcggcggc catctcggct tccttgcgaa tcttcgcggc
     8521 ggccgtgacc gcgtcggccg aaccgtcggc ggcggcggag gcctcgagtc ccgggtctgg
     8581 ttccgctccc aacatactgc caaactgg
//
LOCUS       DAORNX010000069         8592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6105_length_8592_cov_8.176409, whole genome shotgun sequence.
ACCESSION   DAORNX010000069 DAORNX010000000
VERSION     DAORNX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6105_length_8592_cov_8.176409"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="nuoG"
                     /locus_tag="PLO65_04635"
     CDS             complement(<1..1403)
                     /gene="nuoG"
                     /locus_tag="PLO65_04635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRD27569.1"
                     /translation="MPIAKVNGVEVEFEPGMTVLQVAELAGEEIPRFCYHERLSIAGN
                     CRMCLVEVKPGPPKPQASCALPAAENQEIFTNTPMVRKAREGVMEFLLINHPLDCPIC
                     DQGGECDLQDQAMGYGRDDSRYAENKRAVEEKEMGPLIKTVMTRCIQCTRCVRFITEV
                     AGVPEIGLISRGEDVEITTYLGAAVTSELSGNVNDLCPVGALTHKPWTFNYRPWELKK
                     TQSVDVMDALGSAIRVDSRGAQVMRALPRVNEDINEEWLSDKSRYAVDGLGRQRLDRP
                     YVRVNGKLAPATWGEAFAAVAAKVKATSPDRIGVIAGDLQDAESMKAALDLFRSLGVN
                     NLDCRQDGTALGGGPRESWLFNTTLAGIEQADVVLLVGTNPRLEAPVFNARLRKQWLA
                     GKTRFGVIGQPADLTYDYDYLGAGPASITGLARSKSDFVKALKDAKNPAIIIGQGALT
                     RADGGAVLKALAGLART"
     gene            complement(1494..2261)
                     /locus_tag="PLO65_04640"
     CDS             complement(1494..2261)
                     /locus_tag="PLO65_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRD27570.1"
                     /translation="MLRILPLECGTITAPAELFQTGLTGPRTVPVYAFLITHPTRGMA
                     VFDTGLRPDMDGRLLFGSYACSLPAGHDLASRLRAMDVDPDRIGRLVASHAHFDHLGA
                     ADLLPNADIVIHAAELERSLADHPKAHGRVALEMGHRRVLTGDSHDLFGDGSCELFHT
                     PGHTCGHQSLRVKRAGGHDVLAGDACYFCETLEPLAMQPHPENGEVYLATLTRLRAMA
                     RAGDFIVPGHDAAFLAAIPSASALSAPFSPVSGPPDG"
     gene            complement(2269..3588)
                     /gene="nuoF"
                     /locus_tag="PLO65_04645"
     CDS             complement(2269..3588)
                     /gene="nuoF"
                     /locus_tag="PLO65_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRD27571.1"
                     /translation="MVGILEDKDRIFTNLYGLHDWGLEGAKKRGCWNGVKDMLDAGGP
                     WIIDNIKQSGLRGRGGAGFGTGLKWSFMPKEVREGRPHYLVVNADESEPGTCKDREIM
                     RHDPHLLIEGCLIASFAMQAHACYIYIRGEYVREREVLEAAIKQAYEARLIGKNNIHG
                     WDFDLYVHHGGGAYICGEETALLESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESI
                     AVAGTILRRGAGWFAGFGRPNNTGTKLFCVSGHVNLPCNVEEAMSIPFRQLIEDHCGG
                     IRGGWGNLKAVIPGGSSVPMIPAEQCEDLPMDFDALRELRSGLGTAAVIVMDKSTDLV
                     RAIARLSYFYKHESCGQCTPCREGTGWMWRVMERMATGEADMKEIDTLLDVTTQVEGH
                     TICALGDAAAWPIQGLFRHFRHEVEDRITSYRAGKPHVQGATLIAAE"
     gene            complement(3595..3867)
                     /locus_tag="PLO65_04650"
     CDS             complement(3595..3867)
                     /locus_tag="PLO65_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27572.1"
                     /translation="MPDPGEAELLARRAAYIDTLRGLYRRERLAGFFLILAGFALVAP
                     SWWIWNWSHLAIQVGYGLIMLGWVAYIYVIWRRTAWRRANPFDPKA"
     gene            complement(3994..4680)
                     /gene="nuoE"
                     /locus_tag="PLO65_04655"
     CDS             complement(3994..4680)
                     /gene="nuoE"
                     /locus_tag="PLO65_04655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRD27573.1"
                     /translation="MSVRRLAKDQPASFEFSADTLKTANWWIAKYPPERKQSAVLPIL
                     WLIQKQEGWVSEPAIRAVAVLLGMPVIRVLEVATFYTMFQLEPVGNVAFVQLCGTTPC
                     MLCGANDLKKVLEAKIGPKNHVSADGKFSWEEVECLGACSNAPMAAINDYYYEDLTPE
                     SLGQILDEFAAGKKPKPGSRIGRGASEPAGKIQTLLDPKLYDGSAAKKIKIPNLPEKP
                     GKAKKADAPA"
     gene            complement(4677..5888)
                     /locus_tag="PLO65_04660"
     CDS             complement(4677..5888)
                     /locus_tag="PLO65_04660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRD27574.1"
                     /translation="MADDATLPAIPETPVRKFNINFGPQHPAAHGVLRLVLELDGEVV
                     ERVDPHIGLLHRGTEKLMEARTYLQNIPYFDRLDYVAPMNQEHAFCLAIEKLLGVEVP
                     IRGQVIRVLYSEIGRILNHLLNVTTQAMDVGALTPPLWGFEEREKLMIFYERACGARL
                     HSNYFRPGGVHQDLPEALVEDIDAWAAAFPKVCDDIETLITGNRIFKQRNVDIGVVSR
                     DDAIAWGFSGVMVRGSGIAWDLRRSQPYECYNEFEFDIPLGINGDCYDRYLCRMQEMR
                     ESTRIIRQACEMLRKTRGPVMTLDNKVVPPRRAEMKRSMEALIHHFKLYTEGFRTPEG
                     EVYACVEAPKGEFGVFLVSDGTNKPYRCKIRAPGFPHLAAMDFLNKGHMLADVSAILG
                     SLDIVFGEIDR"
     gene            complement(6028..6627)
                     /locus_tag="PLO65_04665"
     CDS             complement(6028..6627)
                     /locus_tag="PLO65_04665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRD27575.1"
                     /translation="MSTDLEALGQAIVANSAGAITSYDVAFGELTVTGPAGRVLQAVT
                     FLRDHPDCRFHQLVDLCGVDYPERPLRFDVVYHMLSLVKNHRIRLKVQTDEDTPVPSI
                     AGEFPAANWFEREAFDMYGVFFSDHPDLRRLLTDYGFHGHPLRKDFPMTGYVEVRYDD
                     ELKRVVYEPVKIAEFRQFDFLSPWEGASYALPGDDKAGS"
     gene            complement(6624..7205)
                     /locus_tag="PLO65_04670"
     CDS             complement(6624..7205)
                     /locus_tag="PLO65_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRD27576.1"
                     /translation="MEEGSPGMGVIVPAGAAGRTTVEGYDPRLHDPYFDGVSQQLADK
                     GFVTAAADDLITWARTGSLMWMTFGLACCAVEMMQASMPRYDLERYGFAPRASPRQSD
                     VMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYYYSYSVVRGCDRVVPV
                     DIYVPGCPPTAEALVYGVLQLQKKIRRTGTIER"
     gene            complement(7175..7552)
                     /locus_tag="PLO65_04675"
     CDS             complement(7175..7552)
                     /locus_tag="PLO65_04675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRD27577.1"
                     /translation="MDAFLLQYLPIVIFLGIATALGVAFILAAAIFAPTAPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVSILFIIFDLEVAFLFPWAVSLMKLPHDQSIFAFWSM
                     MGFLGVLTVGFVYEWKKGALEWE"
     gene            7837..>8592
                     /locus_tag="PLO65_04680"
     CDS             7837..>8592
                     /locus_tag="PLO65_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRD27578.1"
                     /translation="MTRRPLAIRILDAVERFGDRLPDPVFIFVWLIGGLVVASVICAA
                     LGVTAVNPVTQEVLVAKSLLSAGNLRTLLTEMARTFTGFAPLGLVLLVMLGAGVAERV
                     GLLSAAIEGLVRASPKALLTPMVLLIGLLSNHAADSGIVVLPPLAAVVFAAAGRNPIA
                     GLACAFSATIASFAGNPLPGQFDALILGFTEPAARLLDPTWTANLAGDWYFTATGGAV
                     FIAVGWWITDRVVEPRLGPWTPPERPAGATAEPG"
BASE COUNT         1316 a         2829 c         2980 g         1467 t
ORIGIN      
        1 aaggtcctgg ccaggccggc cagcgccttg agcaccgccc cgccatcggc gcgggtcagg
       61 gcgccctggc cgatgataat ggccgggttc ttcgcgtcct tcagggcctt gacgaagtcg
      121 gacttcgacc tggccagacc cgtgatcgag gccgggccgg cgcccaggta gtcgtagtcg
      181 taggtcaggt cggccggctg gccgatcacg ccgaagcggg tcttgccggc cagccactgc
      241 ttgcgcaggc gggcgttgaa caccggcgcc tcgaggcgcg ggttggtccc gaccagcagc
      301 accacgtcgg cctgctcgat gccggccagg gtggtgttga acagccagct ttcgcgcggc
      361 ccgccaccga gcgcggtccc gtcctggcgg cagtcgaggt tgttcacgcc cagcgaccgg
      421 aacaggtcca gcgcggcctt catcgattcg gcgtcctgca gatcgccggc gatgacgccg
      481 attctgtcgg ggctggtcgc cttgaccttc gcggcgacgg cggcgaacgc ctcgccccag
      541 gtcgcgggcg ccagcttgcc gttcacccgc acatagggtc ggtcgaggcg ctggcggccc
      601 aggccgtcga cggcgtagcg gctcttgtcg ctgagccact cctcgttgat gtcctcgttg
      661 acccgcggca gggcgcgcat cacctgggcg ccgcggctgt cgacgcggat cgcgctgccc
      721 agggcgtcca tcacgtcgac gctctgggtc ttcttcagtt cccacggccg gtagttgaag
      781 gtccagggct tgtgggtcag ggcgccgacc ggacacaggt cgttgacgtt gcccgacagt
      841 tcgctggtca cggcagcgcc cagataggtg gtgatttcca cgtcctcgcc gcgcgagatc
      901 aggccgatct cgggaacgcc ggccacctcg gtgatgaacc gcacgcagcg ggtgcactgg
      961 atgcagcgcg tcatcacggt cttgatgagc gggcccatct ccttctcttc gacagcgcgc
     1021 ttgttctcgg cgtagcggct gtcgtcgcgg ccatagccca tggcctggtc ctgcaggtcg
     1081 cactcaccgc cctggtcgca gatcgggcaa tccagcgggt ggttgatcaa caggaattcc
     1141 atcacgcctt cacgggcctt cctgaccatc ggcgtgttgg tgaagatctc ctggttctct
     1201 gcggccggca gcgcgcagga ggcctgcggc ttgggcggtc cgggcttcac ctcgaccagg
     1261 cacatgcggc agttgccggc gatcgaaagg cgctcgtggt agcagaagcg cgggatttcc
     1321 tcgccggcca gctcggccac ctgcaggacc gtcatcccgg gttcgaactc gacctcgacg
     1381 ccgttgacct tggctatggg catatgggct gcactacctg agcaatcgcg cgtcggcgcc
     1441 gatcgaaacg ggcgccgcgt tggtgcgctg tctctatcgc ttcacgcccc gcgtcaacca
     1501 tcggggggtc cggaaacggg ggaaaacggc gccgacagcg cgctggccga cgggatcgcg
     1561 gccaggaagg cggcgtcatg gcccgggacg atgaaatcgc cggcccgcgc catggcccga
     1621 agacgggtca gggtggccag atagacctcc ccgttctccg gatgcggctg catggccagc
     1681 ggctccaggg tctcgcagaa atagcaggcg tcgccggcca gcacgtcgtg cccccctgcc
     1741 cgcttcaccc gcagcgactg gtgaccgcag gtgtggccgg gcgtgtggaa cagttcgcag
     1801 ctgccgtcgc cgaacaggtc gtggctgtcg ccggtcagga cccgccggtg gcccatctcc
     1861 agggccacgc ggccgtgggc cttcgggtga tcggccagcg accgctccaa ctcggcggca
     1921 tggatgacga tgtcagcgtt gggcagcagg tcggccgccc ccaggtggtc gaaatgggcg
     1981 tgggaggcga ccagccgccc gattcggtcc ggatcgacgt ccatggcccg caggcgtgag
     2041 gccaggtcat gcccggcggg caggctgcag gcgtaggacc cgaacagcag cctgccgtcc
     2101 atgtcgggcc gcaggccggt gtcgaacacg gccatgccgc gggtcgggtg ggtgatcagg
     2161 aaggcgtaga ccggcacggt ccgcgggccg gtcagaccgg tctggaacag ctcggcgggc
     2221 gcggtgatgg tcccgcattc gagggggagg atgcgaagca tccgcctcct actccgccgc
     2281 gatcagcgtg gccccttgga cgtgaggctt gccggcgcgg tagctggtga tgcggtcctc
     2341 cacctcgtgg cggaagtggc ggaacaggcc ctggatcggc caggccgccg cgtcgccgag
     2401 ggcgcagatg gtgtggccct cgacctgggt ggtgacatcc aggagcgtgt cgatctcctt
     2461 catgtccgcc tcgccggtgg ccatgcgctc catcacccgc cacatccagc cggtgccttc
     2521 gcggcacggc gtgcactggc cgcagctctc gtgcttgtag aagtagctga ggcgcgcgat
     2581 ggcccggacc aggtcggtgg acttgtccat gacgatgacg gcggcggtgc ccaggcccga
     2641 gcgcagctcg cgcagggcgt cgaagtccat cggcaggtct tcgcactgct cggccgggat
     2701 catcggcacg gacgagccgc ccgggatgac ggccttgagg ttgccccagc cgccccggat
     2761 gccgccgcag tgatcctcga tcagctggcg gaacgggatg ctcatggcct cctcgacgtt
     2821 gcagggcagg ttcacgtgcc ccgagacgca gaacagcttg gtgccggtgt tgttgggccg
     2881 gccgaagccc gcgaaccagc ccgcgccgcg gcgcaggatc gtgcccgcga cggcgatgct
     2941 ctcgacgttg ttgaccgtgg tcggacagcc gtagaggccg gcgccggccg ggaacggcgg
     3001 cttcaggcgc ggctggccct tcttgccttc gaggctctcg agcagcgcgg tttcctcgcc
     3061 gcagatatag gccccgccgc cgtgatggac gtagaggtcg aagtcccagc cgtggatgtt
     3121 gttcttgccg atcagcctgg cttcataggc ctgcttgatc gccgcctcga gcacctcgcg
     3181 ctcgcgcacg tattcgccgc ggatgtagat atagcaggcg tgcgcctgca tcgcgaagga
     3241 ggcgatcagg caaccctcga tcagcaggtg cggatcgtgg cgcatgatct cccggtcctt
     3301 gcaggtgccg ggttccgact cgtcggcgtt gaccaccaga tagtgcggac ggccctcgcg
     3361 gacctccttg ggcatgaacg accatttcag gccggtgccg aagccggccc cgccccggcc
     3421 gcgcaggccg ctctgcttga tgttgtcgat gatccacgga ccgcccgcgt cgagcatgtc
     3481 cttcacgccg ttccagcagc cgcgcttctt cgcgccttcc aggccccagt cgtggagccc
     3541 gtagaggttg gtgaagatgc ggtccttgtc ttcgaggatg ccgaccatgg ttctctaggc
     3601 tttcggatcg aagggattgg cgcgacgcca ggcggtgcgg cgccagatga catagatgta
     3661 ggcgacccag ccgagcatga tcaggccgta gccgacctga atcgccagat gggaccagtt
     3721 ccagatccac caggacggcg ccaccagcgc gaacccggcg agtatcagga agaagccggc
     3781 cagccgctcg cgacgataga ggccgcgcag ggtgtcgata taggccgcgc gcctggccag
     3841 cagttcggct tccccgggat caggcatccc agacaccgct catcccggcg aaggccggga
     3901 cccaggtttg tttgcccacg gctccgcgcc cctgtccgct caggaaaaaa gcctgggtcc
     3961 cggccttcgc cgggatgagc ggaaggtggg agatcatgcg ggcgcgtccg cctttttggc
     4021 cttgccgggc ttctccggca ggttcggaat cttgatcttc ttcgccgccg agccgtcgta
     4081 cagtttcgga tcgagcaggg tctggatctt gccggcgggc tcggaggcgc cccggccgat
     4141 gcgcgagccg ggcttgggct tcttgccggc cgcgaactcg tcgaggatct ggcccaggct
     4201 ctctggcgtc aggtcctcgt agtagtagtc gttgatcgcg gccatcggag cattggaaca
     4261 ggcgcccagg cactcgacct cttcccagct gaacttgccg tcggccgaga cgtggttctt
     4321 cgggccgatc ttcgcctcga gaaccttctt caggtcgtta gccccgcaca gcatgcaggg
     4381 cgtggtgccg cacagttgga caaaggcaac gtttccaaca ggttccagct ggaacattgt
     4441 gtagaaggtg gccacctcca gcacgcggat caccggcatg ccgagcagta cggcgaccgc
     4501 gcggatggcg ggctcggaga cccagccttc ctgcttctgg atcagccaca ggatcggcag
     4561 caccgccgac tgcttgcgct cgggcgggta tttggcgatc caccagttcg cggtcttcag
     4621 cgtgtcggcg ctgaattcga acgaggcggg ctggtctttc gcgagacgac gaacgctcac
     4681 cggtcgatct ctccaaacac gatgtcgagg ctgcccagaa tggcgctgac gtcggccagc
     4741 atgtggccct tgttgaggaa gtccatcgcc gccagatgcg ggaagcccgg cgcgcggatc
     4801 ttgcagcggt agggcttgtt ggtgccgtcg ctgaccagga acacgccgaa ctcgcctttc
     4861 ggcgcctcga cgcaggcgta gacctcgcct tccggcgtac ggaagccctc ggtgtagagc
     4921 ttgaagtgat ggatcagggc ttccatcgag cgcttcatct cggcgcgccg cggcgggacg
     4981 accttgttgt cgagcgtcat caccggcccg cgggtcttgc gcagcatctc acaggcctgg
     5041 cggatgatcc tggtcgactc gcgcatttcc tgcatgcggc agaggtaacg gtcgtagcag
     5101 tcgccgttga tccccagcgg aatgtcgaac tcgaactcgt tgtagcattc gtacggctgg
     5161 ctgcgccgca gatcccaggc gatgcccgag ccgcgcacca tcacgcccga gaagccccag
     5221 gcgatggcgt cgtcgcggct gaccacgccg atgtcgacgt tgcgctgctt gaagattcga
     5281 ttgccggtga tcaacgtctc gatgtcgtcg cagaccttcg gaaaggccgc ggcccaggcg
     5341 tcgatgtcct cgaccagggc ttcgggcaga tcctggtgga cgccgccggg acggaagtag
     5401 ttgctgtgca gacgagcgcc gcaggcgcgc tcgtagaaga tcatcagctt ctcgcgctcc
     5461 tcgaagcccc agagcggcgg cgtcagggcg ccgacgtcca tggcctgggt cgtcacattg
     5521 agcagatggt tgaggatccg gccgatctcg gaatagagca cgcggatgac ctggccgcgg
     5581 atcggaacct cgacgcccag cagcttttcg atcgccaggc agaaggcgtg ctcctggttc
     5641 atcggcgcga cgtagtcgag gcggtcgaag tacgggatgt tctggaggta ggtgcgggcc
     5701 tccatcagct tttcggtgcc acggtgcagc aggccgatgt gcgggtcgac ccgttcgacg
     5761 acctcgccgt ccagctccag caccaggcgc agcacgccgt gcgccgccgg gtgctgcggg
     5821 ccgaagttga tgttgaactt gcgaaccggg gtctccggga tcgccgggag ggtggcgtcg
     5881 tcggccatca ggaatggact cccgtcgagc agcttgcgtg gttcgacacg cgcttcgcgc
     5941 tgctcaccat gacgtggaga gatggcgcat caaaaatgca cgtcatcccg agcaggcccg
     6001 aagggccgtg tcgaaggacg gccatgatca gctccccgcc ttgtcgtcgc caggcagggc
     6061 gtagctggcg ccctcccatg gcgaaaggaa atcgaactgg cggaattcgg cgatcttcac
     6121 cggctcgtag accacccgct tcagctcgtc gtcgtagcgg acctcgacgt agccggtcat
     6181 cgggaagtcc ttgcgcagcg ggtgcccgtg gaagccgtag tcggtcagca gccggcgcag
     6241 gtccggatgg tcgctgaaga aaacgccgta catgtcgaac gcctcgcgct cgaaccagtt
     6301 ggccgccggg aactcgccgg cgatgctggg cacgggcgtg tcctcgtcgg tctgcacctt
     6361 cagccggatg cggtggttct tcaccaggct gagcatgtgg tagaccacgt cgaaccgcag
     6421 cggccgctcg ggatagtcga cgccgcacag gtcgaccagc tggtggaacc ggcaatcggg
     6481 atggtcgcgc aggaaggtca cggcctgcag cacgcggccg gccgggccgg tgacggtcag
     6541 ttcgccgaag gcgacgtcat agctggtgat cgcgccggcg ctgttggcga cgatcgcctg
     6601 gccgagggcc tcgagatccg tgctcatcgc tcgatcgtcc ccgtgcggcg gatcttcttc
     6661 tgcagctgca gcacgccata gaccagcgcc tcggcggtcg gcgggcagcc cggaacgtag
     6721 atgtccaccg gcaccacgcg gtcgcagccg cgcacgacgc tgtagctgta gtagtagtag
     6781 ccgccgccgt tggcgcagct gcccatgctg atgacgtagc gcggctcggg catctggtcg
     6841 tagaccttgc gcagggccgg agccatcttg ttggtcaggg tgccggcgac gatcatcacg
     6901 tcgctctggc gcgggctggc gcgcggcgcg aagccgtagc gctccaggtc gtagcgcggc
     6961 atcgaggcct gcatcatctc gaccgcgcaa caggccaggc cgaaggtcat ccacatcagc
     7021 gagccggtgc gggcccaggt gatcaggtcg tcggcggccg ccgtgacgaa acccttgtcg
     7081 gccagctgct ggctgacgcc atcgaaatag gggtcatgca gccggggatc gtagccctcg
     7141 accgtggttc ggccggcggc gcccgcgggg acgatcactc ccattccagg gctcccttct
     7201 tccactcata gacgaagccc acggtcagca cgccgaggaa gcccatcatc gaccagaagg
     7261 cgaagatgct ctggtcgtgc ggcagcttca tcaggctgac cgcccaggga aacaggaagg
     7321 ccacttccag gtcgaagatg atgaacagga tcgagaccag gtagaaccgg acgtcgaact
     7381 tcatccgcgc gtcgtcgaag gcgttgaagc cgcactcgta ggccgagagc ttctcgggat
     7441 ccggcgccgt gggcgcgaag atggcggcgg cgaggatgaa ggccacgccg agggccgtcg
     7501 cgatcccgag gaagatcacg atcggcaaat actggaggag gaaggcgtcc atgggcatcc
     7561 cggaaaggag tcgcggccct tctagcccat ggccgtgagg ggttcaaact ccgttggggg
     7621 gaccgggacc tgttttcatc gtgatcccgg tcctcctacg cgggatccct ctcgaagccg
     7681 ctcgggccgc gcggtcatgc ccaccgtgcg ggaagcgggc ggacgggtcc cgggcgggag
     7741 gcccgggagg acggtcgttg ggcttgcgag gcggtggtcc ccgtgcagac ttctccccgg
     7801 tcagcccgat tccgggcgcc gccggggggc acggccatga ccagacgtcc gctcgcaatc
     7861 cggatcctcg acgcggtcga gcggttcggc gaccgcttgc cggatccggt gttcatcttt
     7921 gtctggctga tcggcggcct cgtcgtcgcc agcgtgatct gcgccgctct gggcgtaacg
     7981 gcggtcaatc cggtgaccca ggaggtgctg gtcgccaaga gtctgctgtc ggccggcaac
     8041 ctccgcaccc tgctgaccga aatggccagg accttcaccg gcttcgcgcc gctgggcctg
     8101 gtgttgctgg tgatgctcgg cgccggggtg gccgaacggg tcgggctgct gtcggcggcg
     8161 atcgagggac tggtcagggc cagtcccaag gcgctgctga cgccgatggt gctgctgatc
     8221 ggcctgctgt ccaaccacgc ggccgattcg ggcatcgtcg tgctgccgcc gctggcggcc
     8281 gtggtgttcg ccgccgccgg gcgcaatccg atcgcgggcc tcgcctgcgc cttttccgcg
     8341 acgatcgcct ccttcgccgg caatcccctg cccggccagt tcgacgccct gatcctcggc
     8401 ttcaccgagc ccgccgcccg gctgctggac ccgacctgga ccgccaacct tgccggcgac
     8461 tggtatttca ccgccaccgg cggagcggtg ttcatcgccg tcggctggtg gatcaccgac
     8521 cgggtggtcg aaccgcgcct cgggccgtgg accccgccgg aacggcccgc gggcgcgacg
     8581 gcggagcccg gc
//
LOCUS       DAORNX010000070         8563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6121_length_8563_cov_8.008815, whole genome shotgun sequence.
ACCESSION   DAORNX010000070 DAORNX010000000
VERSION     DAORNX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6121_length_8563_cov_8.008815"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLO65_04685"
     CDS             <1..588
                     /locus_tag="PLO65_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRD27579.1"
                     /translation="EARAQDAPAAPEAPGPRDDPEDAYPDFLLSPVPLTPPPPGPRHL
                     KFHHLHTGEDLEAVYWDGGRYVWDALQAVNGLLRDFRSTEIHPIDVRLLDILFTLQAL
                     TGSKEPYRIISAFRSPETNEMLSGRSAATNGATQVAKKSLHMEGKAMDVRLNDVSLTG
                     FRDAALALQGGGVGYYADSAFVHLDIGPVRTWQGT"
     gene            606..941
                     /locus_tag="PLO65_04690"
     CDS             606..941
                     /locus_tag="PLO65_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27580.1"
                     /translation="MAQKSGGGWFWLLVGILAGIAMTLAALLFLNAGSGDVVDNQSVA
                     DEAAASAAGQLPEAPAPVAPAKAETAAAPIPIPHDAGLDPASDDQIADDAAAAGMTSR
                     APPPAPRGE"
     gene            1081..2859
                     /locus_tag="PLO65_04695"
     CDS             1081..2859
                     /locus_tag="PLO65_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD27581.1"
                     /translation="MEPTNTEAPEYFHKVVDCQWACPAHTPVPEYIRLIAQGRHADAY
                     MVNWKSNVFPGILGRTCDRPCEPACRRGRVDEEPVAICRLKRVAADNKGDVAARMPPA
                     PETKIGKRVACIGAGPASMTVARDLAPLGYQVTVYDADGKAGGMIRSQIPRFRLPESV
                     IDEEMGFITGLGLEMRLGQRVDSLASLLEEDYDAIFVGTGAPRGRDLDVPGRHEAAAN
                     IHIGIDWLSSVSFGHIERIGRRVIVLGGGNTAMDCCRTSRRLGGEDVKVIVRSGFEEM
                     KASPWEKEDAMHEGIPILNFLVPKAFTHENGRLTGVTFEKVAPVYDDKGRRSLKPTGE
                     PDQHFECDDVLVAIGQENSFPWIERDIGLTFDDWGMPVVDETTFQSSHPKVFFGGDAA
                     FGPKNIIWAVAHGHDAAISIDHFCRGTDLRLRPPPGVLVSSQKMGIHEWSYDNDVSPE
                     DRQKVPLLPMAQALADVKAEVELGFDDPRSWAETQRCLNCDVQTVFSTPLCIECDACV
                     DICPTDCITFTVNGEEEELRPRLKAPALNLTQDLYVSGTLPTGRVLVKDEDVCLHCGL
                     CAERCPTGAWDMQKFVIEMTHAGGVK"
     gene            2856..4694
                     /locus_tag="PLO65_04700"
     CDS             2856..4694
                     /locus_tag="PLO65_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRD27582.1"
                     /translation="MTRQIQATNDFVVKFANVNGSGSASANGLFVKAILRMGVAVAAR
                     NIFPSNIQGLPTWYEIRVSGNGWLGRRGGVDLMVAMNPQTWDRDVAEIDSGGYLFYDS
                     TKPMPASKFRDDITVVGIPLTALCNAAYAEPSKRKVLKNIIYLGALTYLLGIEPAVVE
                     ALLAEEYASKPALIPANIEAMNIGFNHARANLKPLGLQLKRADAVGDRIFVDGNTAAA
                     LGAVYGGATVCAWYPITPSTSLAEGFIDYCKKLRHDPETGEARYAIVQAEDEIASIGI
                     VVGAGWNGSRAFTCTSGPGVSLMQEFIGLSYFAEIPAVIFDVQRGGPSTGMPTRTQQS
                     DILSAAYASHGDTKHVLLLPEGPNECFEMGAQAFDLADRLQTMVFVMLDLDMGMNEWL
                     IEPFKWDDRKTLDHGKVMTAEMLEAGADFGRYKDVDGDGIPWRTLPATHPSKGAYFTR
                     GTSRDPYARYSEEGAVYVDNMERLLRKFDTAKSLVPAPVLRKASKQTAYGVIYYGSTS
                     PAMDEALSGLEAGGLQVDALRVRAFPFPGEVFDFIAHHELVFVVEQNRDAQLRTLLIN
                     EGGVDPARLVKVLNYDGSPITARFIQAELARGMGAVDVNAAGEMAQ"
     gene            4691..5746
                     /locus_tag="PLO65_04705"
     CDS             4691..5746
                     /locus_tag="PLO65_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRD27583.1"
                     /translation="MTYIVKPQFHHPSLSTNKLGFTRRDYEGRVSTLCAGCGHDSISA
                     AIVQACFEIDLEPHRLAKLSGIGCSSKTPDYFLSASHGFNTVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHAMRRGVNMAYIVENNGVYGLTKGQFSATADKGSRS
                     KKGVVNSDSGIDLVALALQLGATFVGRSFSGDKDQLVPLIKAALTHKGAAFIDVLSPC
                     IAFNNHKGSTKSFDWVREHNEAVNKLDVLLGRAPITASYEPGSLVEVAQHDGSTLLLR
                     KVAGDYDATDRVRAMSYLQERQAVGEIVTGLLYVDPESEDLHDALNTVHRPLNALSNS
                     ELTPGSAALDKINAALR"
     gene            5865..6227
                     /locus_tag="PLO65_04710"
     CDS             5865..6227
                     /locus_tag="PLO65_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HRD27584.1"
                     /translation="MDATSPATRARAKRLRANMNLPERRLWWALRDSRLDGLKFRRQH
                     PLGPFVLDFYCHAARLAVEIDGGSHDIAGRRESDARRDEWLKDRGVHTLRLPAKLLED
                     DPSAALVRIRQAAGRPMH"
     gene            complement(6305..7489)
                     /locus_tag="PLO65_04715"
     CDS             complement(6305..7489)
                     /locus_tag="PLO65_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRD27585.1"
                     /translation="MSRARRIVDAVLAMDMRTCEAELALVNRDFGDRHWQTRGVHLER
                     YGQIKALLDLRPGLKETRKALIGAYFCHEYSYAGAALTNPSITLHPDQSGLVGGAVRV
                     VMSMRAVGEGHISSIAFREGIVTADGEVVLLPEPRVAMAAHACDPPPEGVPEGPVTAC
                     RYDEASLSGTVIFPFTHAQQNGLEDLRLVRFSAGDEAPVWYGTYTAYSGQRIASEMLV
                     TRDFRTFDLLPMSGTAARNKGMALFPRKVAGQYAMIGRQDGENIWFLRSDDLLDWNGG
                     EMIVSPRYPWELVQMGNCGAPIEIAEGWLLLTHGVGAMRKYAIGAVLLDREHPTRVLG
                     RTTAPMISPSDENREGYVPNVVYTCGALAVGRRLVIPYGVADSSIAFCSVDIDELVAE
                     MV"
     gene            complement(7501..>8563)
                     /locus_tag="PLO65_04720"
     CDS             complement(7501..>8563)
                     /locus_tag="PLO65_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27586.1"
                     /translation="LMHRLRALGLHSAETEALAETYSAFVHHAWNDGAGRFRNFMAFD
                     RTWLEDVGSDDSVARAWWSVAVTASEAGSPALRRWARHLAERVAPHLDSFTALRTYAF
                     LVLGLTSLVRADPGLERERQLLERLARGLAGLLGGREGADWRWFEDGLTYDNARLPEA
                     LLRAGQVLDDESLTTAGLGALDWLCGQQTGVGGVFRAVGTGNFGRPRTVDDPFDQQPL
                     EAAATVDACAAAFAATGEVRWLAEARRAFDWFLGKNDLGVPLADPVTGDCYDGLTPHG
                     PNLNRGAESVLSFQLALCALLSLDRIALPGPRPAVKAPMEGRSAARCPSSVTATSESL
                     PTPPASSFAPSTSRPIPSR"
BASE COUNT         1408 a         2961 c         2915 g         1279 t
ORIGIN      
        1 gaggcccggg cccaggacgc gcccgccgcg ccggaagcgc cggggccgcg ggacgatccc
       61 gaggacgcct atcccgactt cctgctgtcg cccgtgcccc tgacgccgcc cccgccgggg
      121 ccgcgccacc tgaagttcca ccatctgcac acgggcgagg acctggaagc ggtctactgg
      181 gacggcggca ggtacgtctg ggacgccctg caggccgtca acggcctgtt gcgcgacttc
      241 cgctcgaccg agattcaccc gatcgacgtg cggctgctcg acatcctgtt cacgctgcag
      301 gccctgaccg gctcgaagga gccctaccgg atcatttccg ccttccgatc gcccgagacc
      361 aacgagatgc tgagcgggcg cagcgcggcg accaacggcg ctacccaggt ggcgaagaag
      421 agcctgcaca tggaaggcaa ggcgatggac gtgcggctca acgatgtgtc gctgacgggt
      481 ttccgtgacg cggcgctggc cttgcagggc ggcggggtcg gctattatgc cgactcggcc
      541 ttcgtacatc tggatatcgg cccggtcagg acctggcagg gcacctaggg ggatcaggac
      601 gacttatggc tcagaaatct ggaggcggct ggttctggct gctggtcggc atcctggccg
      661 ggatcgcgat gaccctggcg gcgctgctgt tcctcaacgc gggatcgggc gacgtggtgg
      721 acaaccagtc ggtggccgac gaggcggccg cttcggcggc ggggcaactg cccgaggccc
      781 ccgcgccggt cgctcccgcc aaggccgaga cggccgccgc gccgatcccg attccgcacg
      841 acgcgggact tgatccggcg tcggacgacc agatcgccga tgacgcggcg gcggccggca
      901 tgacctcgcg cgccccaccg ccggcgcccc gcggcgaata atcgtcgcgg cgggctggat
      961 aggccgttca acgcgggata aaacaactgt tcgagcatcc ggtgcgggct cgggacggcg
     1021 gccctgccgg ggcggccctc cccctcagac acgggagcag ggggagagga gttctacgcg
     1081 ttggagccta cgaacaccga ggcgccggaa tacttccaca aggtcgtcga ttgtcagtgg
     1141 gcctgtccgg cccatacgcc tgtgcccgag tacatccgtc tgatcgccca aggccggcac
     1201 gccgacgcgt acatggtcaa ctggaagtcc aatgtcttcc cggggatcct cgggcggacc
     1261 tgcgatcgcc cctgcgagcc ggcctgtcgc cgcggccggg tcgacgagga gccggtggcc
     1321 atctgccgtc tcaagcgcgt ggccgccgac aacaagggcg atgtcgccgc ccgcatgccg
     1381 ccggcgccgg agacgaagat cggcaagcgg gtcgcctgca tcggggccgg tccggcctcg
     1441 atgaccgtgg cgcgcgatct cgcgccgctg ggctaccagg tcaccgtcta tgacgccgac
     1501 ggcaaggccg gcggcatgat ccgcagccag atcccgcgct tccgcctgcc cgagagcgtc
     1561 atcgacgagg agatgggctt catcaccggc ctgggcctgg agatgcgcct gggccagcgc
     1621 gtcgacagcc tcgccagcct gctcgaggaa gactacgacg ccatcttcgt cggcacgggc
     1681 gcgccgcgcg gccgtgatct cgacgtgcct ggccggcatg aagccgccgc caacatccac
     1741 atcggcatcg actggctgtc gagcgtgtcg ttcggtcaca tcgagcgcat cggccggcgc
     1801 gtgatcgtgc tcggcggcgg caacaccgcc atggactgct gccgcacgtc gcgccgtctt
     1861 ggcggggagg acgtcaaggt catcgtccgg tccggcttcg aggagatgaa ggcctcgccg
     1921 tgggagaagg aggacgcgat gcacgagggc atcccgatcc tcaatttcct tgtccccaag
     1981 gccttcaccc acgagaacgg ccggctcacc ggcgtgacct tcgaaaaggt cgccccggtc
     2041 tacgacgaca agggccgccg cagcctgaag ccgacgggcg agccggacca gcacttcgaa
     2101 tgcgacgacg tgctggtggc catcggccag gagaacagct tcccctggat cgagcgcgac
     2161 atcggcctga ccttcgacga ctggggcatg ccggtcgtcg acgagacgac cttccagtcc
     2221 agccacccga aggtgttctt cggcggtgac gcggcgttcg ggcccaagaa catcatctgg
     2281 gccgtggccc acggccatga cgcggcgatc tcgatcgacc acttctgccg cgggacggac
     2341 ctgcggctgc ggccgccgcc gggcgtgctc gtctcctcgc agaagatggg catccacgag
     2401 tggagctacg acaacgacgt ctcgccggaa gaccggcaga aggtcccgct gctgccgatg
     2461 gcccaggccc tggcggacgt gaaggccgag gtcgagcttg gcttcgatga cccgcgctcc
     2521 tgggccgaaa cgcagcgctg cctgaactgc gatgtgcaga ccgtgttctc cacgccgctg
     2581 tgcatcgaat gcgacgcctg cgtcgacatc tgcccgaccg actgcatcac cttcaccgtc
     2641 aacggcgagg aggaggagtt gcggccccgg ctcaaggcgc cggcgctgaa cctgacgcag
     2701 gacctctacg tctcgggaac cctgcccacg ggccgggtgc tggtgaagga cgaggacgtc
     2761 tgcctgcact gcggcctctg cgccgagcgc tgcccgacgg gcgcctggga catgcagaag
     2821 ttcgtcatcg agatgaccca cgccgggggc gtgaaatgac ccgtcagatc caggccacga
     2881 acgacttcgt ggtcaagttc gccaacgtca acggctccgg ctcggcttcg gccaacggcc
     2941 tgttcgtcaa ggccatcctg cggatgggcg tggccgtcgc ggcgcgcaac atcttcccgt
     3001 cgaacatcca gggcctgccg acctggtacg agatccgcgt cagcggcaac ggctggctcg
     3061 gccgccgcgg cggcgtcgac ctgatggtcg ccatgaatcc gcagacctgg gaccgcgacg
     3121 tcgccgagat cgatagcggc ggttacctgt tctacgactc gaccaagccg atgccggcca
     3181 gcaagttccg cgatgacatc accgtcgtcg gcatcccgct gacggccctg tgcaatgccg
     3241 cctacgccga gccgtccaag cgcaaggtgc tgaaaaacat catctatctg ggcgcgctga
     3301 cctaccttct gggcatcgag ccggcggtgg tcgaggcgct gctggccgaa gagtatgcgt
     3361 ccaagcccgc cctgatcccg gccaatatcg aagccatgaa tatcggcttc aaccacgcca
     3421 gggccaacct gaagccgctc ggcctgcagc tgaagcgggc tgacgccgtg ggcgatcgca
     3481 tctttgtcga tggcaatacc gcggcggctc tcggcgcggt ctatggcggg gccaccgtct
     3541 gcgcctggta tccgatcaca ccctcgacct cgctggcgga aggcttcatc gactactgca
     3601 agaagctgcg ccacgatccg gagaccgggg aggcgcgcta cgccattgtc caggccgagg
     3661 acgagatcgc ctcgatcggc atcgtcgtcg gcgccggctg gaacggctcg cgggccttca
     3721 cctgcacctc ggggccgggc gtctcgctga tgcaggagtt catcggcctc agctacttcg
     3781 cggagatccc cgcggtgatc ttcgacgtgc agcgcggcgg gccctcgacg ggcatgccga
     3841 cccgcaccca gcagtcggac atcctctccg ccgcctacgc cagccacggc gacaccaagc
     3901 atgtcctgct gctgccggag gggccgaacg agtgcttcga gatgggggcc caggccttcg
     3961 atctggccga ccgcctgcag accatggtct tcgtgatgct cgacctcgac atgggcatga
     4021 acgagtggct gatcgagccc ttcaagtggg acgaccgcaa gaccctcgac cacggcaagg
     4081 tgatgaccgc cgagatgctc gaggcgggcg cagatttcgg tcgctacaag gacgtcgacg
     4141 gcgacggcat tccgtggcgc accctgccgg cgacccatcc gtcgaagggg gcctatttca
     4201 cccgcggcac ctcgcgcgac ccctacgccc gctacagcga ggagggggcc gtctatgtcg
     4261 acaacatgga acgcctgctg cgcaagttcg acacggcgaa gtcgctggtg ccggcgccgg
     4321 tccttcgcaa ggcctcgaag cagaccgcct acggcgtgat ctattacggc tcgacctccc
     4381 cggcgatgga cgaggccctc tccggcctcg aggccggcgg cctgcaggtc gacgcccttc
     4441 gtgtgcgcgc cttccccttc ccgggcgagg tgttcgactt catcgcccat cacgagctgg
     4501 tcttcgtggt cgagcagaac cgcgacgccc agctgcgcac cctgctgatc aacgagggcg
     4561 gggttgatcc cgcccggctg gtgaaggtgc tcaactacga cggctcgccg atcacggcgc
     4621 gcttcatcca ggccgagctg gcccgcggaa tgggcgcggt cgatgtcaac gccgccgggg
     4681 agatggccca atgacctaca tcgtcaagcc gcagttccat cacccgtcgc tgtcgaccaa
     4741 caagctgggc ttcacgcgcc gcgactacga gggccgggtc tcgaccctgt gcgccggctg
     4801 cggccacgac tcgatcagcg ccgccatcgt ccaggcctgt ttcgagatcg acctcgagcc
     4861 gcaccgcctg gccaagctgt caggcatcgg ctgctcgtcc aagacgcccg actacttcct
     4921 cagcgccagc catggcttca acaccgtgca cggacggatg ccgtcggtgc tgaccggcgc
     4981 caatctggcc aaccgcgagc tgatctatct gggcgtctcc ggcgacggcg acagcgcctc
     5041 gatcggcctc ggccagttcg cccacgccat gcggcgcggg gtcaacatgg cctacatcgt
     5101 cgagaacaac ggcgtctacg gcctgaccaa gggccagttt tcggccaccg ccgacaaggg
     5161 cagcaggtcc aagaagggcg tggtcaattc cgacagcggc atcgacctgg tcgccctggc
     5221 cctgcagctc ggcgcgacgt tcgtcggccg cagcttctcg ggcgacaagg accagctggt
     5281 gccgctgatc aaggcggccc tgacgcacaa gggcgcggcc ttcatcgacg tgctgagccc
     5341 ctgcatcgcc ttcaacaacc acaagggctc gaccaagagc ttcgactggg tgcgcgagca
     5401 caacgaggcg gtcaacaagc tcgacgtgct gctcggccgc gcgccgatca ccgcgtccta
     5461 cgagcccggc tcgctggtcg aggtggccca gcacgacggc tcgaccctgc tgctgcgcaa
     5521 ggtggccggc gactacgacg cgaccgaccg ggtgcgggcg atgagctacc tccaggaacg
     5581 ccaggccgtc ggcgagatcg tcaccggcct gctctatgtc gatccggagt cggaagacct
     5641 gcacgatgcg ctcaacaccg tgcatcggcc gttgaacgcc ctgtcgaaca gcgagctgac
     5701 ccccggctcg gcggccctcg acaagatcaa cgcggccctg cgctaacctc tccctcttgg
     5761 gagaggggga ccacccgaag ggtggtggag tgggaggcgc ggtggcggcc ggcgcgcgcg
     5821 ccgcgtgtat tgcatgatcg cataaactat ggttgtgttg agccatggac gcaacctcac
     5881 ccgccacccg ggcgcgagcc aagcgcctca gagccaacat gaatctgcct gaacgccgac
     5941 tatggtgggc gttgcgcgac agccgcctcg acgggctcaa gttccgccga cagcacccgc
     6001 tcggaccgtt cgtcctggat ttctattgcc acgccgcccg gctggccgtg gagatcgatg
     6061 gcggatcgca cgacatcgcc ggccgacggg aaagcgatgc ccgacgcgat gaatggctga
     6121 aagaccgtgg cgttcacacg ctgcgcctgc ccgcgaagct gcttgaagat gacccaagcg
     6181 cggcgctggt gcggatacgt caggcggcgg ggcggccgat gcactaagct ttccggccat
     6241 caccgcgcgt cccactccac catgcttcgc atggtccccc tctcccaagt gggagaggta
     6301 gatttcagac catctccgcc accagctcgt cgatatcgac cgagcagaag gcgatcgagc
     6361 tgtcggcgac gccgtagggg atgaccagcc gacggccgac ggccagggcg ccgcaggtgt
     6421 agacgacgtt gggcacatag ccctcgcggt tctcgtcgga cggcgagatc atcggcgccg
     6481 tcgtgcgacc cagcacgcgg gtcggatgct ccctgtccag cagcaccgcg ccgatggcgt
     6541 atttgcgcat ggccccgacg ccgtgggtca gcaacagcca gccctcggcg atctcgatcg
     6601 gcgcgccgca gttgcccatc tggaccagct cccaggggta acgcggcgag acgatcatct
     6661 ccccgccgtt ccagtcgagc aggtcgtccg agcgcaggaa ccagatgttc tcgccgtcct
     6721 ggcggccgat catcgcgtac tggccggcga ccttgcgggg gaacagggcc atgcccttgt
     6781 tgcgggccgc cgtgccgctc atcggcagca ggtcgaaggt gcggaagtcg cgcgtcacca
     6841 gcatttccga ggcgatcctc tggccgctgt aggcggtgta ggtgccgtac cagaccgggg
     6901 cctcgtcgcc ggccgagaat cgcaccagcc gcaggtcctc cagcccgttc tgctgggcgt
     6961 gggtgaaggg gaagatcacc gtcccggaca gcgacgcctc gtcgtaacgg caggccgtga
     7021 ccggtccctc gggcacgccc tcgggcgggg gatcgcaggc gtgggccgcc atggcgacgc
     7081 gcggttcggg cagcagcacc acctcgccat cggcggtgac gatcccctcc cgaaaggcga
     7141 tcgaggagat gtgaccctcg cccacggccc gcatcgacat gaccacccgc acggccccgc
     7201 cgaccagccc cgactggtcg gggtgcaggg ttatgctggg attggtcagg gccgcccctg
     7261 catagctgta ctcgtgacag aaataggccc cgatcagcgc cttccgcgtt tccttgaggc
     7321 ccggccggag gtcgagcagc gccttgatct ggccgtagcg ctcgaggtgc acgccgcgcg
     7381 tctgccagtg ccggtcgccg aagtcgcggt tgaccagggc cagttcggcc tcacaggtgc
     7441 gcatgtccat cgccagcacg gcatcgacga tccgtcgcgc gcgcgacacc tgtcccgcga
     7501 tcagcggctg gggatcgggc gcgatgtgga aggggcgaat gacgacgcgg gcggggttgg
     7561 gagggattct gaggtcgctg tgacggagct tgggcatcgg gcggcggaac ggccttccat
     7621 gggagctttg acggcgggac gggggccggg cagggcgatg cgatcgagcg acagcagggc
     7681 gcacagcgcc agctggaacg acagcaccga ttccgccccg cggttgaggt tgggcccgtg
     7741 cggcgtgagg ccgtcgtagc agtcgccggt gaccgggtcg gccaggggga cgcccagatc
     7801 gttcttgccg aggaaccagt cgaacgcgcg ccgagcctcg gccagccagc ggacttcgcc
     7861 ggtcgcggcg aaggcggcgg cgcaggcgtc gactgtggcg gcggcctcca gcggctgctg
     7921 gtcgaacgga tcgtcgacgg tgcggggccg gccgaagttg ccggtgccga cggcgcggaa
     7981 caccccgccc acgccggtct gctggccaca gagccagtcg agcgccccca ggccggccgt
     8041 tgtcaggctc tcgtcgtcca gcacctggcc cgctcgaagc agcgcctcgg gaagccgggc
     8101 gttgtcataa gtcaggccgt cctcgaacca gcgccagtcg gcgccttcgc ggccgccaag
     8161 caggccggcc agtccccggg cgagccgctc gagcagctgg cgttcgcgct ccagccccgg
     8221 gtcggcccgc accagactgg tcagtccgag cacgagaaag gcgtaggtcc gcagggcggt
     8281 gaagctgtcc agatggggcg cgaccctctc ggccagatgg cgggcccagc ggcgcagggc
     8341 gggagaaccg gcctcggagg cggtgaccgc gacggaccac caggcccggg cgacgctgtc
     8401 gtccgaaccg acatcctcga gccaggtccg gtcaaaggcc atgaagttgc ggaaccggcc
     8461 ggccccgtcg ttccaggcgt ggtggacaaa cgcggaatag gtctcggcca gggcctccgt
     8521 ctcggcggag tgcaggccga gggcgcgcag gcgatgcatc agc
//
LOCUS       DAORNX010000071         8519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6155_length_8519_cov_8.746810, whole genome shotgun sequence.
ACCESSION   DAORNX010000071 DAORNX010000000
VERSION     DAORNX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6155_length_8519_cov_8.746810"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..851
                     /locus_tag="PLO65_04725"
     CDS             423..851
                     /locus_tag="PLO65_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRD27587.1"
                     /translation="MTTRPLLFDSPFLLGFDHTRALIERAAKAAAEGYPPYNVEARDD
                     GDVRITLAVAGFSPDQLEIQVEGNQLTVAGRRGDGPEAADKAYLHRGIAARGFIRTFV
                     LADGMVVRGALLEHGLLHVDLARPEPERMIQRIAIRTAAE"
     gene            929..1186
                     /locus_tag="PLO65_04730"
     CDS             929..1186
                     /locus_tag="PLO65_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HRD27588.1"
                     /translation="MTPQLLTNEAFAALGAPDLVYVRPVTAAEIIADTPVAALRGFEL
                     SPEQTLYAVHRADGERLAVLGDRDSAVAAALAHELAPVSVH"
     gene            complement(1213..1677)
                     /gene="ptsN"
                     /locus_tag="PLO65_04735"
     CDS             complement(1213..1677)
                     /gene="ptsN"
                     /locus_tag="PLO65_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HRD27589.1"
                     /translation="MNIGNLLEPRAIAPRAGGGGKRQVLSAVADIAARNYGLDANQVL
                     DALLEREAAGSTGVGHGVAVPHARLPGLDRMRGVFLRLEQPTAFESVDDQPVDLVFAL
                     LAPVGSDTEHLRSLARVSRLLRKSDVRQQLRQARTAEAIYALLAREPQSTAA"
     gene            complement(1731..2351)
                     /gene="raiA"
                     /locus_tag="PLO65_04740"
     CDS             complement(1731..2351)
                     /gene="raiA"
                     /locus_tag="PLO65_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRD27590.1"
                     /translation="MQVQVSGKHVDVGEALRSRVSDDITTTIGKYFDRGGSADVVVTK
                     EGYAFRVDCSIRLDSGQMLQSQGLAGDAHAAFSAALDKIETRVRRYKRRLKSHSVQAE
                     ARRDETAALYVLRAPDDEVEDWDGVDDHAPPTGMIIAESEATLETMTVSMAVMQLDLT
                     DSQTIVFRNAAHGGLSVVYRRPDGNIGWIDPERTQSQSGNGKRVVA"
     gene            complement(2428..3048)
                     /locus_tag="PLO65_04745"
     CDS             complement(2428..3048)
                     /locus_tag="PLO65_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRD27591.1"
                     /translation="MDPVDRRRPLSDLLTEISDDPDPVVTIGEIVHRLGRRSFGALLF
                     IFAAPNWLPLPPGSSTFLAAPLILLAPQIALGIRGPWLPRFIDQRELKRSDLAGALRR
                     LVPPLQRVEKVSRPRLVFLFGPIGDRLIGLTVTLLSLVLLLPIFLGNMAPAAAIAAFG
                     LALVQRDGILALIGYGVAGVSVGLLVVGWRVAAAFLTHIGHVLGLV"
     gene            complement(3101..4609)
                     /gene="rpoN"
                     /locus_tag="PLO65_04750"
     CDS             complement(3101..4609)
                     /gene="rpoN"
                     /locus_tag="PLO65_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRD27592.1"
                     /translation="MALGPRLELRQGQSLVITPQLQQAIKLLQLTNLELEAYVEGELE
                     RNPLLARDDRDGEAEAEPTRVETPDYSADQLPDGAAQADMDAGPSDLSPGERDTGDAD
                     TSEPGRAGGDIDWSRAGRGATFEGEDSLEGRLTRDRTLGEHLHDQLAVARLSEPQRVV
                     AAVLIDAVDDGGYMRADLVEMAERNRQQGADQLAFIEEVLTVLQGFEPTGVMARDVRE
                     CLSLQLRERDRLDPAMAALLDNLDLLARRDMAGLRRVCGVDDEDLREMIGELKALTPR
                     PGAAFGGEPSQPVVPDVFVREGSGGLWLVELNTDTLPKVLVDRRYHARIAGGARTDQE
                     KTFVQDCLASANWLVKSLDQRARTILKVSSEIVRQQDGFLAYGVEHLRPLNLKTVADA
                     IGMHESTVSRVTSNKYIATPRGVFELKFFFTSSIASSSGGEAHSAEAVRHKIRQLIDA
                     EGDVGDVHSDDAIVDILKEAGVDIARRTVAKYREAMRIPSSVERRRMLRASA"
     gene            4770..5246
                     /locus_tag="PLO65_04755"
     CDS             4770..5246
                     /locus_tag="PLO65_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD27593.1"
                     /translation="MPRIDIATTPERRGSGYPAPFDAPCADRVRQRLGEAGGLTDFGV
                     NLMRLPPGGWSSQRHWHSHEDEFVYVIEGELTLVEDGGATLLRAGDCAAFPKGAGDGH
                     HLINRSDAMAIYLEVGSRHPADLTTCSDIDMMSSNADGRFVHKDGTPYPWVNRSPR"
     gene            complement(5216..5965)
                     /gene="lptB"
                     /locus_tag="PLO65_04760"
     CDS             complement(5216..5965)
                     /gene="lptB"
                     /locus_tag="PLO65_04760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRD27594.1"
                     /translation="MAADSDQNGEGLVVDRLVKTFGERRVVRGVSLRLRRGEVAGLLG
                     PNGAGKTTCFYMITGLIPVDEGSILLDGRDITALPMYQRARMGVGYLPQEASIFRGMT
                     VEENVLAVVEMREKEAARAREVTQALLDELRISHLKTAPAVSLSGGERRRVEIARALA
                     SEPSFMLLDEPFAGIDPLAIADIRDVIGYLKGRGIGILITDHNVRETLDIIDRASIIH
                     AGEVLFEGAPQEIVDNPEVRRVYLGDRFTQG"
     gene            complement(5966..6508)
                     /locus_tag="PLO65_04765"
     CDS             complement(5966..6508)
                     /locus_tag="PLO65_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRD27595.1"
                     /translation="MIKSGARMQSAIGNLGWAALAAAGVLLGASGAQAQLDPRAPIDI
                     TADSSEVIQSQCQSTWSGAVEALQGRTRLRADSVRMYSLKKGDGCGASDRMEAEGQVY
                     FVTPERTVRADSAVYTFSGQTITLTGNVIVVQGKSVVRGDRLVINTRTGQAQMSSNTT
                     GRNQTGRVRGVFYPDQGTTN"
     gene            complement(6517..7140)
                     /gene="lptC"
                     /locus_tag="PLO65_04770"
     CDS             complement(6517..7140)
                     /gene="lptC"
                     /locus_tag="PLO65_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRD27596.1"
                     /translation="MTATPHPVSAPIDREAAFARWERRRALIRGLRRGLPAVMALITL
                     ALITMVTMSALKAPHPVKSDDPIIRMVGARFQGRVEDGRSFLIGAQQAARDERQPNKV
                     TLVEPIMVVGAETETPRRLMARMGVYDEQTRMLQLTGEVRIDDGLGNRVASNDITIDT
                     RTGTAVGQKGIEGDGPTGQVSADSYSVKDKGDRIEFQGRVRTRVNPE"
     gene            complement(7146..7763)
                     /locus_tag="PLO65_04775"
     CDS             complement(7146..7763)
                     /locus_tag="PLO65_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRD27597.1"
                     /translation="MANHLHEGDIPAGLFAGVASVAIDSETMGLSLQRDPLCLVQISA
                     GDGDAHVVRLNRPAYDCPNLKALLVDPAVLKIFHFGRFDIAMFERHLGITTTPVWCTK
                     IASRLARTYTDRHGLKDVCKELLGVDLSKAQQSSDWGAATLSPEQLAYAASDVLYLHG
                     LKARLQAMLDREGRAALAQACFDFLPQRARLDLAGWEEVDIFAHS"
     gene            complement(7810..7896)
                     /locus_tag="PLO65_04780"
     tRNA            complement(7810..7896)
                     /locus_tag="PLO65_04780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7860..7862),aa:Leu,seq:cag)
     gene            8034..>8519
                     /locus_tag="PLO65_04785"
     CDS             8034..>8519
                     /locus_tag="PLO65_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRD27598.1"
                     /translation="MRGLVTVFGGTGFVGRQVVRSLARQGWRVRVAARNVGRGYRLRM
                     LGDVGQIEVVQANLRNPGSVGRALDGAEACVNLVGVLFESGRQGFQALHAMGAKTVAE
                     AAAARGVTRFVQISALGADAASASKYARTKAEGEAAVRAAVPTAVILRPSVVFGPEDD
                     FF"
BASE COUNT         1285 a         2938 c         2956 g         1340 t
ORIGIN      
        1 caacgccgcc caggccgaac agaccgaggg ccgccacccc ggcgctgatc agggccatca
       61 ggctgtagag cgtggccccc acggcgagca cacgggagag gcgttcgcga ccggccggcg
      121 gcgcctcgcc ggactgcagg gtcatggtca tggtgcgttt cccttcgcag ccggccggcg
      181 tctgcgcgcc ctggccgggt tgagtccgtc gccgccccgc ggacggctcg acgggtcgcc
      241 tggaaggtga acccctttgc gccgggattc aaccgacagg ggaatgaccg gcgaccgtgg
      301 tcggcgtcga tcaccgtctt gcgtcgcggc ccgccaatcg ccacctatca ggacaagccg
      361 ccggacggac gcccgacagg ggccggcccg gcgggagtcg cttgtgcgag gactctgaga
      421 cgatgacgac acgacccctg ttgttcgaca gcccgtttct cctgggcttc gatcacaccc
      481 gcgccctgat cgagcgggcc gccaaggccg ccgccgaagg ctacccaccc tacaatgtgg
      541 aagcccgcga cgacggtgat gtgcgcatca ccctggctgt ggccggcttt tcgcccgacc
      601 agctcgagat ccaggtcgag ggcaaccagc tgaccgtggc cggtcggcgt ggcgacggcc
      661 ccgaggccgc cgacaaggcc tatctccatc gcggcatcgc cgcccggggg ttcatccgga
      721 ccttcgtgct ggcggacgga atggtggttc gcggcgccct gctggaacac ggtctgctgc
      781 atgtcgatct ggcccgcccc gagcccgaac gcatgatcca gcggatcgcc attcgcacgg
      841 cggccgaatg accggaaaca gaatgccagc ccggccggct gaaccggcgg ccgcccgctg
      901 cgttcaccta gtgaaggagg tggtcgaaat gacgccccaa ctgcttacga atgaggcctt
      961 tgccgccctt ggcgcgcccg acctggtcta tgtccggccg gtgaccgccg ccgagatcat
     1021 cgccgacacg ccggtggcgg cgctgcgcgg cttcgaactc agtcccgaac agaccctcta
     1081 cgccgtccac cgcgcggatg gcgaacggct ggccgtgctc ggggatcggg actccgccgt
     1141 cgccgccgcc ctggcccacg aactggcgcc cgtgtccgtc cactagtccg aattcaccag
     1201 aggcggccgc cgtcaggcgg ccgttgactg cggctcgcgc gccagcaggg cgtagatggc
     1261 ctcggccgtg cgggcctgac gaagctgctg gcgcacgtcg ctcttgcgca acaggcgcga
     1321 cacccgggcc agggagcgca ggtgttcggt gtccgatccc acgggggcca gcagggcgaa
     1381 caccaggtcc accggctggt catcgaccga ttcgaacgcc gtcggctgct ccaggcgcag
     1441 gaacacgccg cgcatccggt ccaggcccgg caggcgcgcg tgcggcacgg cgacaccatg
     1501 cccgacgccg gtggagccgg cggcttcgcg ctcgagcagg gcgtcgagca cctggttcgc
     1561 atccaggcca tagttgcggg ccgcgatatc ggcgaccgcc gacagcacct gacgctttcc
     1621 accgcctccc gcgcgcggcg cgatggcgcg cggttcgagc agatttccaa tattcatacg
     1681 ctcaaggctc gcaagattca gaccggcgcc ggaaggcggg ccgcgccgtg tcaggcgact
     1741 acccgttttc cattgccgct ctgtgactga gtgcgttctg ggtcgatcca gccgatgttt
     1801 ccgtccggcc ggcgatacac cacggacaat cccccgtggg cggcgttcct aaacacgatt
     1861 gtctgggaat cagtcaagtc cagctgcatg accgccatcg aaacggtcat tgtttccagc
     1921 gtcgcttcgg attccgcgat gatcatgccc gtcggcgggg cgtggtcgtc gacgccgtcc
     1981 cagtcctcga cctcgtcgtc aggcgcgcga aggacgtaca gggcggccgt ctcgtcgcgc
     2041 cgggcctcgg cctgcaccga atggctcttg agtcgccgct tgtagcggcg gacgcgggtc
     2101 tcaatcttgt cgagcgcagc gctgaaggcc gcatgggcgt ctccggccag gccctggctc
     2161 tgcagcatct ggccggaatc gagccggatc gagcagtcga cccggaaggc gtagccctcc
     2221 tttgtgacga cgacgtcggc ggaaccgccg cggtcgaagt atttgccgat ggtggtggtt
     2281 atgtcgtccg agacccgcga acgcagggcc tccccgacgt cgacgtgctt gccggagact
     2341 tgcacttgca tgggaataga acgcgcccgc gagcgggagg tgtcaagccg gccgcgcgct
     2401 gctcgccggt ttgtgagcgg cgggccgtca gaccaggccc aggacgtggc cgatatgggt
     2461 gagaaaggcc gccgcgaccc gccagccgac gaccagcagg cccacgctga cccccgccac
     2521 gccgtagccg atcagggcca ggatgccgtc gcgctggacc agggccaggc caaaggcggc
     2581 gatcgccgcg gcgggggcca tgttgccgag gaagatcggc agcagcagca ccagcgacag
     2641 cagggtcacc gtcaggccga tcagccggtc gcctatcggg ccgaacagga acaccagccg
     2701 cggtcgcgag accttttcga cccgctgcaa cggcggcacc agccgccgca gggctccggc
     2761 caggtcggat cgcttcagtt cgcgctggtc gatgaaccgg ggcagccacg gcccgcgaat
     2821 gcccagggcg atctgcggag ccagcaggat cagcggcgcg gccaggaagg tcgaggagcc
     2881 cggcggcagg ggcagccagt tgggcgcggc gaagatgaac agcagggcgc cgaaagaccg
     2941 ccgccccagc cggtgcacga tctcgccgat cgtcacgacc ggatcaggat cgtcgctgat
     3001 ctccgtcagg aggtcggaaa gcggccggcg gcggtcaacg ggatccatgg gcgacaacat
     3061 acccggttcg caacgcgcga acgagccatc cggtccgcga tcaggccgag gcccgcagca
     3121 ttcgccggcg ttcgaccgag gacgggatgc gcatggcctc tcgatacttg gccaccgtgc
     3181 ggcgggcgat gtcgacgccc gcctccttga ggatgtcgac gatcgcgtcg tccgaatgga
     3241 cgtccccgac atcgccctcg gcatcgatca actggcggat cttgtgccgc accgcctcgg
     3301 ccgagtgcgc ctcgccgccg gaagaggagg cgatcgagga ggtgaagaag aacttcagtt
     3361 cgaacacgcc cctgggggtg gcgatgtact tgttggaggt gacccggctg accgtgctct
     3421 cgtgcatgcc gatggcgtcg gccacggtct tcaggttcag cggtcgcaga tgctcgacgc
     3481 cataggccag gaagccgtcc tgctgccgca cgatctcgct cgacaccttg agaatggtgc
     3541 gggcccgctg gtcgaggctc ttgaccagcc agttggcgct cgccaggcag tcctggacga
     3601 aggtcttctc ctggtcggtg cgcgcgccgc ccgcgatgcg ggcgtgatag cgccggtcga
     3661 ccagcacctt gggcagggtg tcggtgttca gctcgaccag ccacagtccg cccgagcctt
     3721 cgcgcacgaa cacgtcgggc acgacgggct gcgacggctc gccgccaaag gccgcgcccg
     3781 gccggggcgt cagggccttc agctcgccga tcatctcgcg caggtcctcg tcgtcgacgc
     3841 cgcagactcg ccgcagcccg gccatgtcgc ggcgcgccag caggtcgagg ttgtcgagca
     3901 gggcggccat ggccgggtcg aggcggtcac gttcgcgcag ctgcagcgac aggcactcgc
     3961 gcacatcgcg ggccatcacg ccggtcggct cgaagccctg caggaccgtg agcacctcct
     4021 cgatgaaggc cagctggtcg gcgccttgct ggcggttgcg ctcggccatc tcgaccaggt
     4081 cggcgcgcat gtagccgccg tcgtcgaccg cgtcgatcag caccgccgcc acgacccgct
     4141 gcggctcgct gaggcgggcc acggccagct ggtcatggag atgctcgcca agcgtcctgt
     4201 cgcgggtcag gcggccttcc aggctgtcct cgccctcaaa tgtcgcgccg cggccagcgc
     4261 gggaccagtc gatgtcgccg ccggcccggc cgggctcgct ggtgtcggcg tctccggtgt
     4321 cgcgctcgcc cggcgacagg tcggaggggc cggcgtccat gtcggcctgg gccgcgccgt
     4381 cgggcagctg gtcggcggaa tagtcaggcg tctcgacccg ggtcggctcg gcttccgcct
     4441 cgccgtcgcg gtcatcccgc gccagcaggg gattgcgctc cagctcgccc tcgacatagg
     4501 cctcgagctc gaggttggtc agctgcagca gcttgatcgc ctgctgcagt tgcggcgtga
     4561 tgacgaggct ctggccctgt ctcagctcaa gtcttggacc gagcgccacg cgcttccccc
     4621 acccattgaa acaaccgggg gccagcttgg acggcggacg gttaacgcgc cccttaagcg
     4681 gcgcggttct tgcacggcgc cggggcggcg ggtctggcca cggggtcgcc ccgcgctgtc
     4741 tggcccaaaa ggtcgcggga tgagccgcca tgccaaggat cgacatcgca acgacgcctg
     4801 aacgtcgggg ctcggggtac ccggctccgt tcgacgcccc ctgcgccgac cgcgtccgcc
     4861 aacgccttgg cgaggccggc ggcctgacgg acttcggggt caacctcatg cgcctgccgc
     4921 cgggaggatg gtcgagccag cggcactggc attcgcatga agacgagttt gtctatgtga
     4981 tcgagggcga gctgacgctg gtcgaggatg gcggcgcgac tctgctgcgc gccggtgact
     5041 gcgcggcctt tcccaagggc gcgggcgacg gccatcatct gatcaacagg tccgacgcca
     5101 tggcgatcta tctcgaggtc gggtcgcgcc atccggccga cctgacgacc tgctcggaca
     5161 tcgacatgat gagctcaaac gcagacgggc ggttcgtcca caaggacggg acgccctatc
     5221 cctgggtgaa ccggtcgccc aggtagaccc ggcggacttc cgggttgtcg acgatttcct
     5281 gcggcgcgcc ctcgaacagc acctcgccgg catggatgat cgaggcgcga tcgatgatgt
     5341 cgagcgtctc gcgcacattg tggtcggtga tcaggatgcc gatgccgcgg cccttgaggt
     5401 agccgatcac gtcgcggatg tcggcgatgg ccagggggtc gatgccggcg aacggctcat
     5461 ccagcagcat gaagctgggt tcgctggcca gggcccgggc gatttccacg cgccgccgct
     5521 cgccgccgga gagcgacacg gccggagcgg tcttcaggtg actgatgcgc agctcgtcca
     5581 gcagggcctg ggtcacctcg cgggcccggg cggcttcctt ctcgcgcatc tcgaccacag
     5641 ccaggacgtt ttcctcgacc gtcatgccac ggaagatcga cgcctcctgg ggcaggtaac
     5701 ccacgcccat ccgggcgcgc tggtacatcg gcaaggcggt gatgtcccgg ccgtcgagca
     5761 ggatcgagcc ttcgtcgacc gggatcaggc cggtgatcat gtagaagcag gtggtcttgc
     5821 ccgcgccgtt gggtcccagc aggccggcga cctcgccgcg ccgcaaccgc aggctgacgc
     5881 cgcgcaccac ccgccgctcg ccgaacgtct tgacgagccg gtcgacgaca aggccttcgc
     5941 cgttctggtc gctatcggcc gccacctagt tggtggtccc ctggtccggg taaaacaccc
     6001 ctcgcacgcg gccggtctgg ttgcggccgg tggtgttcga ggacatctgc gcctggccgg
     6061 tgcgggtgtt gatgaccagc cggtcgccgc gcaccacgct cttgccctgc acgacgatga
     6121 cattgccggt cagggtgatg gtctggcccg agaaggtgta gacggcgctg tcggcccgca
     6181 cggtgcgctc gggggtgacg aaatacacct ggccctcggc ctccatgcga tcgctggcgc
     6241 cgcagccgtc gcccttcttg aggctgtaca tgcggaccga gtcggctcgc agccgcgtgc
     6301 gtccctgcag ggcctccacg gcgccgctcc aggtcgactg gcactggctc tggatcactt
     6361 ccgacgaatc ggcggtgatg tcgatcggcg cgcgcggatc gagctgcgcc tgggcgccgc
     6421 tcgcgccgag cagtacgccc gccgcggcca gagccgccca tccaagattt ccaatcgcgc
     6481 tctgcatgcg cgctcccgac ttgatcatga cccgtcttat tccggattga cgcgcgtgcg
     6541 aaccctgccc tggaattcga tgcggtcgcc cttgtccttg acgctgtagg agtccgccga
     6601 cacctgtccg gtcggaccat ctccctcgat ccccttctgg ccgacggccg tgcccgtccg
     6661 ggtgtctatg gtgatgtcgt tggaggccac gcggttgccc agcccatcgt cgatccgcac
     6721 ctcgcccgtc agctggagca tgcgggtctg ttcgtcgtag acgcccattc gggccatcag
     6781 acggcgcggg gtttcggttt cggccccgac gaccatgatc ggctcgacca gggtgacctt
     6841 gttcggctgg cgctcgtcgc gcgccgcctg ctgcgcgccg atgaggaagc tgcggccgtc
     6901 ctcgacacgg ccctggaaac gggcgccgac catgcggatg atcgggtcgt cggacttgac
     6961 cgggtgcggc gccttgaggg cgctcatcgt gaccatggtg atcagcgcca gggtgatcag
     7021 ggccatcacc gccggcaggc cgcggcgcag gccccggatc agcgcccgcc gacgctccca
     7081 gcgcgcgaac gccgcctcgc ggtcgatcgg ggcggacacg gggtggggcg tcgcggtcat
     7141 gcgcgtcagc tgtgtgcgaa gatatcgacc tcttcccagc cggcgaggtc gagccgcgcg
     7201 cgctggggca ggaagtcgaa acaggcctgg gccagcgccg cgcgaccctc gcggtcgagc
     7261 atcgcctgca gcctggcctt caggccgtgc agatagagga cgtcagaggc ggcgtaggcc
     7321 agctgttcgg gcgacagggt cgccgcgccc cagtcggagc tctgctgcgc cttggacagg
     7381 tccacgccaa gcagctcctt gcacacgtcc ttcagcccgt ggcggtcggt gtaggtgcgg
     7441 gcgagcctgg aggcgatctt ggtgcaccag acgggggtgg tggtgatccc cagatgccgc
     7501 tcgaacatgg cgatgtcaaa gcggccgaag tgaaagatct tcagcacggc gggatcgacc
     7561 agcagcgcct tgaggttggg gcagtcgtag gccggccggt tcaaccgcac cacgtgggcg
     7621 tcgccgtcgc cggccgagat ctgcaccagg cacagcggat cgcgctgcag gctcaggccc
     7681 atggtttccg aatcgatggc cacggacgcg acgccggcga acaggcccgc cgggatgtcg
     7741 ccttcatgca gatgattggc caaatgcggt cgctccctct ctcactctct accatatcga
     7801 agaggagggt ggtgcccagg agaggactcg aacctccacg gccgttaagc cactggcacc
     7861 tgaagccagc gcgtctacca attccgccac ctgggctcgc atacctttcc ggaagcccgg
     7921 atcagcgcga aggcgagcct catagggcgc cgccttgatg cggtcaatgg cgcgttgcac
     7981 atggaccccg cctcgtctat ccgggcgcaa tcgtgcaggg aaaaggcgcc gtcatgcgtg
     8041 ggctcgtcac cgtcttcgga ggaaccggct ttgtcggccg tcaggttgtc cggtcgctgg
     8101 ccagacaagg ctggcgggtg cgggtcgccg cgcgcaatgt cggccggggt tatcgcctgc
     8161 ggatgctcgg cgacgtcggc cagatcgagg tggtccaggc caacctgcgc aaccccggct
     8221 ccgtcggccg ggcgctggac ggcgccgagg cctgcgtcaa cctggtcgga gtgttgttcg
     8281 agagcggccg tcagggcttc caggcgctgc acgccatggg cgcgaagacc gtcgccgagg
     8341 ccgccgccgc gcgcggtgtg acgcggttcg tgcagatttc ggccctcggc gcggacgccg
     8401 cgtcggcgtc gaaatacgcc cgcaccaagg ctgaaggcga ggccgccgtg cgcgccgccg
     8461 tgccgaccgc ggtgatcctg cggccgtcgg tcgtgttcgg tcccgaagac gatttcttc
//
LOCUS       DAORNX010000072         8510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6164_length_8510_cov_7.650266, whole genome shotgun sequence.
ACCESSION   DAORNX010000072 DAORNX010000000
VERSION     DAORNX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_8510_cov_7.650266"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLO65_04790"
     CDS             <1..690
                     /locus_tag="PLO65_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD27599.1"
                     /translation="GTGPFPVALQLHGCAGCQPFQNSYAEAAVKAGVAVVIVDSLRPR
                     NMSRLDGSLFVCTGTVLRGARRAGDLYAMLHWLKDQPWADATRVVAAGWSHGGWTIMD
                     AFAQGEGAARMTGLADAAPEQLKAVRGAFLVYPYASFPSLTSARGWSATPKVFAVLGG
                     RDQVVGVAGPLKALNRLRADGLSVDVLTFPDATHAFDDDDANDPRTRYRPDLRATAEG
                     YYVAALRSAFS"
     gene            complement(794..>1320)
                     /locus_tag="PLO65_04795"
     CDS             complement(794..>1320)
                     /locus_tag="PLO65_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRD27600.1"
                     /translation="PFEQKAVARRAALFVKDGAPEALATSIAAIQPLTTAAELVDLAG
                     ALGWPVAGTARLYHQVGAAFSFDRLRAAAGEKRGGDHFERMAVRRLIEDMLAEQAQVA
                     RAVMTFAGSPDSAVDNDAAKATVASWAQMHSGPGRVVRRTIEEIEQAGGGWTFAKLTI
                     ANAALRELAAVAGK"
     assembly_gap    1321..1383
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1384..5631)
                     /locus_tag="PLO65_04800"
                     /pseudo
     CDS             complement(1384..5631)
                     /locus_tag="PLO65_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621883.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
     gene            complement(6028..7224)
                     /locus_tag="PLO65_04805"
     CDS             complement(6028..7224)
                     /locus_tag="PLO65_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRD27601.1"
                     /translation="MTRAPAKSAATSSGKAAKAAPAGARPAEGDPVTVRPEGIARNAL
                     VILAVIASAAALYWMRGILTPLALAMFLAIMIDSFARRLEHRWPRLPDSVSFLAAIVL
                     SLLIFGGTLVVVADRASGFIVELAGYGPRLDELIARLAAMVGVQAPPPLTQMVDDLNP
                     AKYVGEVAQGLQGVVSDAVFVLIYLGFIIASRRGFKRKIVALFPHNQERSDALEIFNR
                     IRFGVERYLWVQTVTGLMIAAGSWLAMIAVGLENALFWAFLIFLAAYIPIIGGFIGIV
                     FPAVFGLVQFDTLWQGFVLLGVLYGIQFVVGNIITPRMQGDSLNIDPVVVLLALALWG
                     ALWGLPGMFLSTPLAVMAMIILAEFDGSRWIAILMSENGDPQGVDEEGRHRRRGKRFG
                     QADDAP"
     gene            7344..>8510
                     /locus_tag="PLO65_04810"
     CDS             7344..>8510
                     /locus_tag="PLO65_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRD27602.1"
                     /translation="MDAARDPATLFVGFSDKPETLRLDRANRHGLVAGATGTGKTVTL
                     QILAQGFSDAGVPVFAADVKGDLSGISQPGTPGDKFLARARSMNLTLEPAAPPTVFWD
                     LFGEQGHPVRTTVSEMGPLLLARMLELNEVQEGVLNIVFKVADAEGLLLLDLKDLQAA
                     LKYVAENEKSIDAEYGNVSAATVGTIQRGLLTLETQGGANLFGEPALALSDLMRVDGS
                     GRGLVSVLAADRLIQSPRLYATFLLWLLSELFEDLPEIGDPEKPRLVFFFDEAHLLFR
                     DAPKALLEKVEQVVRLIRSKGVGVFFVTQNPADIPETVLAQLGNRFQHALRAYTPAEQ
                     KGLRAAAASFRPNPAFDTATAIQDLGVGEALVSVLDERGAPTVTARTMIRPPASR"
BASE COUNT         1279 a         2920 c         3006 g         1242 t
ORIGIN      
        1 gggaccggcc cgtttcccgt cgcgctgcag ctgcatggct gcgccggctg ccagccgttc
       61 cagaacagct acgccgaggc ggcggtgaag gccggggtcg cggtggtcat cgtcgacagc
      121 ctgcggccgc gcaacatgtc gcggctcgac ggcagcctgt tcgtctgtac gggaaccgtc
      181 ctgcgcggcg cccggcgggc cggcgacctc tacgccatgt tgcactggct gaaggaccag
      241 ccctgggccg acgcgacccg cgtggtcgcg gccggctgga gccacggcgg ctggaccatc
      301 atggacgcct tcgcccaggg ggagggcgcc gcgcggatga ccggcctggc cgacgccgcc
      361 ccggagcagc tgaaggccgt gcgcggagcc tttctcgtct acccttacgc cagcttcccc
      421 tccctgacct cggcgcgtgg ctggagcgcg acgccaaagg tgttcgccgt gctgggcggc
      481 agggaccagg tggtgggcgt ggccggcccg ctcaaggcgc tgaaccggct cagggccgac
      541 ggcctgtcgg tcgacgtcct gaccttcccg gacgccaccc acgccttcga cgacgacgac
      601 gccaacgacc ctcgcacccg ctatcgcccg gacctgcggg cgacggcgga ggggtactat
      661 gtggcggcgc tgcggagcgc gttcagctag cagcttgcgt ccttcgacac ggccctgcgg
      721 gcctgctcag gatgacgtaa cttggtgcat gtcatggtga gcagcgccga aggcgcgtgt
      781 cgaaccacgc accctatttc ccggccaccg ccgccagttc ccttaacgcc gcgttggcga
      841 tggtcagctt ggcgaaggtc cagccgccgc cggcctgctc gatctcctcg atggtccggc
      901 gcacgacccg tccgggtccg gagtgcatct gggcccagga cgccaccgtg gccttggcgg
      961 cgtcgttgtc gaccgccgag tccgggcttc cggcgaaggt catcaccgcg cgggcgacct
     1021 gggcctgttc ggccagcatg tcctcgatca gacggcggac agccatgcgc tcgaagtgat
     1081 cgccgccgcg cttttcgccg gccgccgccc gcagccggtc gaagctgaag gccgcgccga
     1141 cctgatggta cagccgcgcc gtgccggcca cgggccagcc cagggctccg gccaggtcga
     1201 ccagttcggc cgcggtggtg agcggctgga tggcggcgat actggtcgcc agggcctcgg
     1261 gcgcgccgtc cttgacgaac agcgccgccc ggcgggccac cgccttctgc tcgaacggcg
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnngcccggg ccggcgcggc gggccagcca gaaggtctgg ctgcggatga tgtgggcaag
     1441 ggcccggaac agggccagct gaccctcggc cggcgccttg ccgtcgagcg cggccaccgc
     1501 gtcccagctg acctggaagt cgaggatccg ccgggccgcc tcgaagccgg tgaccagggc
     1561 gcccgtgtcg cagccggccg ccgcccgcag gcgcgcgggg aaggtcggac cgcagatatt
     1621 gaccagctcg ttgtcgatca ccgtggcgat gatctcgcga cgcagccggt gacgcttcat
     1681 ctcgtcgtcg aacaccccga gggggccggg gaaataggcc tccatggtgg cctcgaaggc
     1741 cggatcgtcg ggggccttgg aggcgacgat ctcgtcgaac aggtcgagct tgccataggc
     1801 cagcagcacg gccagttcgg gccgggtcag ccccttgcgg gctgtggcca gctcgagcat
     1861 ctgggccgcg tcgggcaggc cctcgacctt gcggtccagg cggccggacg cctcgagttc
     1921 ggccatgaag cgcgcgtgat tctcgacctc gccggcggcg tcctgctcga gcaacgacag
     1981 cgccagcgtc tggtcgtagt tgtgcgccag cacatggtga ccgacctcgt cggtcatcga
     2041 ggccagcagc cggtcgcggg ccgggcggtc cagcttgccg gcccgctcgg ccatgccggt
     2101 gaggatcttg atgttgacct cgtggtcgga gctgtcgacc ccggctgaat tgtcgatggc
     2161 gtcggtgttg atccgcccgc ccgcacgcgc gaactcgata cggccggcct gggtcaggcc
     2221 caggttggcg ccttcgccga ccaccttgac ccgcaggtcg gccgcgttga cgcggatcgc
     2281 gtcattggcc ttgtcgccgg cctcggcgtt gctctcgccg cgcgacttca cataggtgcc
     2341 gatgccgccg agatagagca gctcggccct ggccttgaga atcgccgtca tcagttccgc
     2401 cggcgaggcg gactcggcct tgatgtcgag caccgcccgg atttccggtg acagcgggat
     2461 cgacttgagc gcgcgggaga agaccccgcc cccggccgag atcaggctcc tgtcatagtc
     2521 ctcccaggac gagcgcggca gctcgaacat ccgcttgcgc tcgggccacg acgtcgcggc
     2581 gtcagggttg gggtcgacga agatgtgccg gtggtcgaag gccgcgacca gccggatgtg
     2641 cttgctcagc agcatgccgt tgccgaacac gtcgcccgac atgtcgccga cgccgacgac
     2701 ggtgaagtcg gtcgtctgga tgtccttgcc ggcctcgcgg aagtggcgct tgaccgcttc
     2761 ccaggccccg cgggcggtga tgcccatgac cttgtggtcg tagccggccg agccgcctga
     2821 ggcgaacgcg tcgccgagcc agaagccgta ggattcggcc accccgttgg cgatgtcgga
     2881 aaaggtcgcc gtgcccttgt cggccgccac caccagatag gggtcgtcgc cctcgtgggc
     2941 gatcaccgcc ggcggccgga cgacatggtt gtcggcgtcg atattgtcgg tgatgtcgag
     3001 caggccgaac aggaaggtct gataagcccg gatgccctcc ccccggacgg cgtcgggggt
     3061 cccgccgcgc ggcaggtgct tgggatagaa gccgcccttt gaaccgaccg gcacgatgac
     3121 cgcgttcttg acctgctgcg ccttgaccag gcccagcacc tctgtgcgga agtcgtcgcg
     3181 gcggtccgac cagcgcaggc cgccgcgggc caccgggccg aagcgcaggt gcacgccctc
     3241 gacatgcggg gcccagacga agatctcgcg gaacggcttg ggggccggaa ggtcctcgag
     3301 ctcgcggctg gcgatcttga agctgatgtg cggctttggc ccgccgtcgt cggcggtctg
     3361 gtagaaattg gtccgcttca gcgccccgac cagcagggcc agacggcgca gcacgcggtc
     3421 gtggtcgaga ctgtcgaccg cctgcagggc catggtgatc tcgtccatca ccgccgcggc
     3481 ctgcccctcg cgggtcttga gatcgctggt gatcgccggg tcgaacttga tgcggaacag
     3541 gtcgaggatc agccgcgcga cccctggatg ttcggacagg gcctgttcct gcaccagctg
     3601 gctgggatcg agccccgact gctggcgata gcgcgccagg gcgcggatca gcgccgcttc
     3661 gcgccaggag acgcccagct cgagcaccag gcgattgaaa ccgtcgcttt cggtccgtcc
     3721 ggtccagacc gccgtgaagg ccgcctcgaa ggcgtcccgc acgtcattga acaccagatg
     3781 ctcgccctgc tcgtcctcga gaaggaactc gtgcacccag accagctccc ggtcgccgcc
     3841 ggcggccagc ggggtcagct cgaaggcgtc ctcgatgagg gccttcagcc ccatgtgctc
     3901 gagaatcggc atgacctcgg ccagcggggc cgcgccgccc ttgcggtaga ccttgaagcg
     3961 gaaccgggtg gccgggtcgc cgaccacgcg gaaggcgcgg gtgcggaagc tttcgccggc
     4021 catcgcgtcg atctcggcga ggtcggccag cgcctcctcg gcgggatagt attcgcggta
     4081 gccgctgctg aaggcgcccg accagcgggc gagggcgccg gtcacgccgg cggcgggcgg
     4141 actggcccgg acggcggcct cgaaacggtc gctccaggtg cgcgccgcct cggcgatatc
     4201 ggcctcgacc aggcccaggt cgggatccat gtggcggccg ggatcgaccc ccagaatgaa
     4261 gtggacgcgc gccagcggcg cgtcggagaa gctcggataa taggccgaaa cgcggccctg
     4321 ataggcctcg gcgaggatcc ggccggcctt ctcgcgcact cccgagtcgt atcgctcgcg
     4381 cggcacgaac agcagcaccg agatgaaccg gtcgaacgga tcacggcgcg cgaacagccg
     4441 cacgcgcggg cggtcgaaca ggtgcagcac gcccatcgcc agcaccagca gctcgtcttc
     4501 cggcgtctgg aacagctcgt cccgcggcca ggtctcgagg atgttgcgca ggcgtttgtc
     4561 gttgtggccg ccggccgaga ccccggcccg ggcgatgacg ttggccacct tgcgccggac
     4621 cagcggcacc tcgcgcgccg gcgcgtcata ggcctcggcg gtgaacaggc cgacaaagcg
     4681 gacttcgccc gcgggcttgc cgtccgcgcc gtaccgcttg accccgacat agtccatgta
     4741 gccgcggcgg tggacccgcg agcgcaggtt cgacttggcc acgatcagcg gcgagtcggt
     4801 ctccaggtag cggcgaagct gcttggacag gatggccggc tcgctgctgc ggcgcaggac
     4861 gcggcgactc tggtcgcgca gcacccccag gctgtcttcc ggccgataga ccggctcctc
     4921 ggcggcgtag tcgccgtcgg ccgtgcgcgg atagtcgtag acccgggccc cgaggaagac
     4981 gaaacggtcc cgttccagcc agccgaggaa ggccaggtcc tcttccagcg cctcggccga
     5041 caccggcgcg gcggccccgg ccaggccggt gatggtttcc cgcatcagcg cctgcatggc
     5101 ggcgaagtcg gcgaccgcca tctgcacgtc gcccatggcc gcgcgaacgc cgtcgagcag
     5161 gggggccgtc cggtcctcgc cgaccgggtc cagatgaacc tggatgaccg acagcttccg
     5221 gccgtcgcgc tcgaccagcg ggtggaacat ggcccgcacc ggaaagccgg cctcggcaat
     5281 ctcgcccatg atcgaatcga cgagaaacgg ccggtcgggc tgggcgatct cgagcaggtc
     5341 gcggcccaga tcggccccgt cggcgtccgt ccaccggcgc agcctgatcg ccggcgcatc
     5401 accctcaagc gcctggccat agcgccacag ctcggcgaag gcggcggcca ggtcggccag
     5461 gctgacggcc ggcagttcct cgatcgacag gtcgcgatgg acctgggcga tgaaggccgc
     5521 ttccgcgccc ttgggcgggc cggacctgtg gcccagggcc ctggcgaagg cgttggcgag
     5581 gctgtcggag tcgaaccccg gcgcggccgg gtgaaggctt gcgcccttca tcaagtatcc
     5641 ccgtaggatg gtcccgccga gacgcccccc gccgaccggc gccggtcgga cgatctgaac
     5701 gtgctcaagg gaagtgactt ttgcaggccc ttttagcctg atcgcgcgga aagtttcgat
     5761 caggatcggc gtcagcctag accgcttggg gcgccgatca accgcctggg gccgcctcaa
     5821 gcaagaactt tccgcagggg cgccgtggcc tggatacgaa tacgccgccg gaggggggat
     5881 ccggcggcgc tcgtcgtccg cgccgggggg aggggcggga cgattttctt ccgggcgccg
     5941 ccttcggggg ggaggaaggg cgccatccgg tccggcggca ggggagtgcg accggacact
     6001 caacagatag gagtcgttat tcgcctttca aggggcgtcg tcggcctggc cgaatcgttt
     6061 tccgcgccgc cggtggcgcc cctcctcgtc gacgccctgg gggtcgccgt tctcggacat
     6121 caggatggcg atccagcgcg atccgtcgaa ctcggccagg atgatcatcg ccatgaccgc
     6181 cagcggcgtg gacaggaaca tgcccggcag gccccacagc gcgccccaca gggccagggc
     6241 cagcagcacc accaccggat cgatgttgag cgaatcgccc tgcatccgcg gggtgatgat
     6301 gttgccgacg acaaactgga ttccgtagag caccccgagc aggacgaagc cctgccagag
     6361 ggtgtcgaac tggaccagac cgaacaccgc gggaaagaca atgccgatga acccgccgat
     6421 gatcgggatg taggcggcca agaagatcag gaaggcccag aacagcgcgt tctccagccc
     6481 cacggcgatc atcgccagcc aggagccggc ggcgatcatc aggccggtga ccgtctggac
     6541 ccagaggtag cgctcgaccc cgaaccggat ccggttgaag atttccagcg cgtcggaacg
     6601 ctcctgattg tgcgggaaca gggccacgat cttgcgcttg aagccgcgcc gcgaagcgat
     6661 gatgaagccc aggtagatca gcacgaagac cgcgtccgac accacgccct gcaggccctg
     6721 ggcgacctcg ccgacgtatt tcgccgggtt caggtcatcg accatctggg tcagcggcgg
     6781 cggggcctgg accccgacca tcgccgccag ccgggcgatc agctcgtcga gcctgggacc
     6841 gtagccggcc agttcgacga tgaagcccga ggcccggtcg gcgaccacca cgagcgtgcc
     6901 gccgaagatc agcagcgaca gcacgatggc ggcgagaaac gacacgctgt cgggcaggcg
     6961 cggccagcgg tgctcgagcc gccgtgcgaa gctgtcgatc atgatcgcca ggaacatggc
     7021 cagggccagc ggcgtgagaa tgccgcgcat ccagtagagg gcggcggccg aggcgatcac
     7081 cgccaggatc accagcgcgt tgcgcgcgat gccctcgggg cggacggtta ccgggtcgcc
     7141 ctcggcgggc cgcgcgccgg ccggtgcggc cttcgccgcc ttgccggacg atgtcgcggc
     7201 cgacttcgcg ggcgccctgg tcatggggtc tctcctcagt tccggggccg agtctcgggt
     7261 ccggccgtgc gtccgtcaat gcgcgcgctt ggcgaagcgg gatgcggccc tgtagcctgt
     7321 gacaaaccac ctggagacga cgaatggacg ccgcccgcga tcccgcaacg cttttcgtcg
     7381 gcttttcgga caagcccgag acgctgcggc tggaccgggc caatcgccac ggcctggtcg
     7441 ccggggcgac cggcacgggc aagaccgtca ccctgcagat tctcgcccag ggcttttccg
     7501 acgccggggt gccggtgttc gccgccgatg tgaagggcga cctgtcggga atcagccagc
     7561 ccggaacgcc gggggacaag ttcctcgccc gggcccggtc gatgaacctg acgctcgagc
     7621 cggccgcgcc gccgacggtg ttctgggacc tgttcggcga gcagggccac ccggtgcgaa
     7681 cgaccgtctc ggagatgggg ccgctgctgc tggcccggat gctcgagctc aacgaggtgc
     7741 aggaaggcgt gctcaatatc gtcttcaagg tcgccgacgc cgagggcctg ctgctgctcg
     7801 acctcaagga cctgcaggcg gcgctgaagt acgtcgccga aaacgagaag agcatcgacg
     7861 ccgagtacgg caacgtctcg gcggcgaccg tgggcacgat ccagcgcggg ctgctgaccc
     7921 tggagaccca gggcggggcc aacctgttcg gtgaaccggc gctggccctg tcggacctga
     7981 tgcgggtgga cggatcggga cggggcctgg tcagcgtcct ggccgccgac cggctgatcc
     8041 agagcccccg gctctacgcc accttcctgc tgtggttgct gtcggagctg ttcgaggacc
     8101 tgccggagat cggcgacccg gagaagccgc gactggtgtt ctttttcgat gaggcccacc
     8161 tgctgttccg cgatgcgccg aaggcgctgc tggagaaggt cgagcaggtg gtgcgactga
     8221 tccgctccaa gggcgtgggc gtgttcttcg tcacccagaa cccggccgac attcccgaga
     8281 ccgtgctggc ccagctcggc aaccgcttcc agcacgccct gcgggcctac accccggccg
     8341 agcagaaggg gctgcgggcg gcggcggcct cgttccggcc caacccggcc ttcgacacgg
     8401 ccacggcgat ccaggacctg ggcgtgggcg aggcgctggt gtcggtgctc gacgagcgcg
     8461 gcgcgccgac ggtcacggcc cgcacgatga tccggccgcc ggcctcgcgg
//
LOCUS       DAORNX010000073         8452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6200_length_8452_cov_8.051566, whole genome shotgun sequence.
ACCESSION   DAORNX010000073 DAORNX010000000
VERSION     DAORNX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_8452_cov_8.051566"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..731
                     /gene="flbT"
                     /locus_tag="PLO65_04815"
     CDS             306..731
                     /gene="flbT"
                     /locus_tag="PLO65_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HRD27603.1"
                     /translation="MPLKLSLKPGERFVLNGAVVQNGDRRGVLILQNKASVLREKDIM
                     QEEEATTPARHVYFPVMMMYLDEPNALRYYDEFVRRLSEFMGVIRNPDVLADCVAISR
                     HAMAREYYKALMLCRKLIEYEDERLGNVAAGVPAGRGAG"
     gene            691..1053
                     /gene="flaF"
                     /locus_tag="PLO65_04820"
     CDS             691..1053
                     /gene="flaF"
                     /locus_tag="PLO65_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HRD27604.1"
                     /translation="MSLQAYQQAAARAENPRDAEYRLFGQVTRALLQAAEVPPEDVRG
                     RIDALDWNRRLWSALASDCADPQNVLPEALRAQIISLSLWVSRHTSAVIRREEDIDPL
                     IDINRIMMQGLSGQPEAA"
     gene            1293..2123
                     /locus_tag="PLO65_04825"
     CDS             1293..2123
                     /locus_tag="PLO65_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRD27605.1"
                     /translation="MLSNSVNTNVGALVALQNLNATNMELSQTQSRINTGKKVATAKD
                     NGAIWAIAQTQRGNSQALNAVRDSLDRGRSTIDVAVAAGESVSDLLNQMKEKALAASD
                     STLDSTSRSALNEDFKALRDQIAKAVSNADFNGTNLIKTGATALAALANADGSSKLTV
                     AAEVMALGGSIVTVAAAGSIGTATLATAMISTVNTSIANTNAALARLGTKSKAFDLHT
                     TFIGKLQDSLDAGVGNLVDADLAKESAKLQALQTKQQLGVQALSIANSSTQTLLSLFQ
                     "
     gene            2385..3215
                     /locus_tag="PLO65_04830"
     CDS             2385..3215
                     /locus_tag="PLO65_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRD27606.1"
                     /translation="MALNSVNTNTGALVALQNLNATNSELNVVQGRINTGKKVASAKD
                     NGAIWAIAQTQRGTSQALNAVKDSLQRGQSTIDVAIAAGESVSDLLVQMKEKALAASD
                     TTLDTASRSALSEDFKALRDQIAKAVSNADFNGSNMIKASGATVTALANADGTSKITV
                     AAQSLALGGANVTVAATASLSTATIAAAMVTTVSTSITNVGAALAKLGTGSKSLEAHL
                     TFVGKLQDSLDAGVGNLVDADLAKESAKLQALQTKQQLGVQALAIANTSTSALLGLFR
                     "
     gene            3336..3620
                     /locus_tag="PLO65_04835"
     CDS             3336..3620
                     /locus_tag="PLO65_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27607.1"
                     /translation="MKTNVTPALSIQDRGAFPASPFAGPDAVKPPPETPRQNADLRLV
                     IEEDQTSGTFVYKTLDRRTGEVVKQLPREEVVRLKDAPGYAPGAVIDAQS"
     gene            3796..4629
                     /locus_tag="PLO65_04840"
     CDS             3796..4629
                     /locus_tag="PLO65_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRD27608.1"
                     /translation="MSISVHTNQSALVALQNLNRTNDELAGAQNRISTGLKVQGPKDN
                     ASVWGIAQSQRADVGALGAVKMSLDRAQSISDVAISAGESISDLLLSMKEKVTAAMDP
                     SLDSVSRTALDADYKSLLRQITQVVNDAEFDGANMLDGSLGAGIRFLANADANSFITL
                     TAKNMSLGGSIITLGAASSITTATLATTIQGLLSTSIANVNQALGDMGAQSRQVGSHN
                     TFVSKLTDVLNSGVGNLVDADLAKESARLQALQVQQQLGAQALSIANSAPQIVLSLFK
                     G"
     gene            4688..5242
                     /gene="pseH"
                     /locus_tag="PLO65_04845"
     CDS             4688..5242
                     /gene="pseH"
                     /locus_tag="PLO65_04845"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HRD27609.1"
                     /translation="MIELRDLVPEDEDRLFQWRGEPDVARWMSDADVSGREAHRAWFD
                     QLRVDPDLRGWLIESGGRPSGLLTLTGLTGHHRRAAWNWFIGDREARGRGVGRAAQVL
                     GLDQAFGVLGLHKVWAEVMADNDAAMKVQTGAGFRREGYLRGHVLKDGRPRDVVMLGI
                     LAEEWAVLAPGARRLLADAGLIAA"
     gene            5310..8207
                     /locus_tag="PLO65_04850"
     CDS             5310..8207
                     /locus_tag="PLO65_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27610.1"
                     /translation="MAVTFDSSILLNYYQSRSGATALTGGGAASSGGTTTKYAPTPPW
                     ATNSGVARASDLVKSAMSGRRFIDEGSAKLDLEGASADYKKLFALYQGLNTLYGLGER
                     LQAKGVSTTEKNRIAEIFTRGLGEISTYTDKARLDQMRLTRGDTMISDKSAVGVPRAK
                     YQYLTGKVYSGAQGDAVPAFAGDVAFTLTVKRGTVTHSIDINLADMGAQDRTMGAVTS
                     FINGKLEAEGLQTRFSVVRTPGEPRKVTTGGTTVTLPAVADDFAFRITGDSTEQATFS
                     AATTKPAVYVTTYAGNPDPDKDIKTDDGVFETSLVKVDGTGIGTEGARVFSSTLEGTI
                     ESVRTSRVGADGALYVLADVNTSVDGQTVKGERDVALLKYDSTGQLMYARTLGAAEDA
                     QGLALTVADDGRVAIAGKVTGALAGATDGPINSGADTGLSDSFVTVFDAKGDEAWTAR
                     RGALKDDEATAVAFGEGGLVYVAGRTKSTQPGGGAALGDWDGYLTAFAADAKGVPRAL
                     FTNQFGTAGSDTVSGIVVDGATVTLAGVESGRGVLRSFSVTPTTTTTEKIVNDGVTTV
                     NVTTTTNGASNTTSNVYGAPDGSGTSSTTTTVHTSAASAVAGATRDLGDLLGGQIAGI
                     GLDGGDLWVAGTTRNAALSVAGTSHAYNGGFDAFAARISADLSSASQDQLTYFGGSTD
                     DTVTAMAVADGKVWVAGRAAADQPGATAIATKDGYLAQLDVGAGTATVQRLTGKDGMA
                     TATSIAIDQSGASALDRLGLPKGTLLYADSARIASATAARAGDTFQIRTSEGGRLATV
                     TLEAGDTLDSLAVKVRRAAGFKARIEVVSDGDFRRLKITPATEGATVEVLPGKGGADA
                     LEAIGLTAGVIRNTVIDKDGKSVSADGGGPVFGLGLPAELDVSTEAGVKNALAVLSKS
                     LGKIRDAYRQLEAAARPQTSAARSANGPVPAYLTGQIANYQAALDRLTGGG"
     gene            8266..>8452
                     /locus_tag="PLO65_04855"
     CDS             8266..>8452
                     /locus_tag="PLO65_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27611.1"
                     /translation="MEYLKDRRIVLAIGGAVALLLGIIFAATIMGRDKGPDKPPPAAT
                     GGLVVETGRDDDTRLDPA"
BASE COUNT         1470 a         2907 c         2847 g         1228 t
ORIGIN      
        1 cgctggcctc gccggccatg cgcgggcggg tggcgacgga gggcgattcg gggaagccga
       61 gtgcggcggc cgagacggcg gacttcgcgg cgaactgcgg cggcatgcgg aggaaccctc
      121 tcggggtggg cgggagcctt ccagatcgct cttttatgct taatcccgcc ttaagagggg
      181 ctcggcgacc caacggcgca caacaccccg ttaaccatgc ggctttagcc ttctttcaac
      241 agggcgggcg gattctcgac gacatgttgg cagccgcgcc gcacgccatc cggagctcgt
      301 tcgtcgtgcc gctgaaactg tcgctgaaac cgggggagcg cttcgttctc aatggcgccg
      361 tcgttcagaa cggcgaccgg cgcggcgtgc tgatcctgca gaacaaggcc tcggtcctgc
      421 gcgagaagga catcatgcag gaggaggagg cgacgacgcc ggcgcgtcac gtctacttcc
      481 cggtgatgat gatgtatctc gatgagccca acgcccttcg ctactacgac gaattcgtca
      541 ggcggctctc ggagttcatg ggcgtgatcc gcaatccgga cgtgttggcc gactgcgtgg
      601 cgatttcccg gcatgccatg gcgcgcgagt actacaaggc gctgatgctc tgccggaaac
      661 tgattgagta tgaagacgaa aggttgggga atgtcgctgc aggcgtacca gcaggccgcg
      721 gcgcgggctg agaatccgcg cgacgccgag tatcgcctct tcggacaggt gacccgcgcc
      781 ctgcttcagg ccgcggaagt gccccccgaa gacgtgcgcg gtcgtatcga tgcgctcgac
      841 tggaaccggc gtctgtggtc ggccctggcg tcggactgcg ccgatcccca gaacgtcctg
      901 cccgaggctc tccgggcgca gatcatttcc ctcagcctct gggtcagccg ccacacgagc
      961 gcggtgatcc gccgcgagga agacatcgat ccgctgatcg atatcaaccg gatcatgatg
     1021 cagggtctgt cggggcagcc ggaggccgcc tgacgtcgcc tccagcgtgg catgacgcga
     1081 acggtccggc aaaagtttcc gggcggtcga atgcgtcccg cccggccccg cgcgcgccgc
     1141 gttccgggta gccaagtctc aaggatttca acggcctgcc gcgcctgccg gcgggccgtt
     1201 ttctggcccg ggccttgcga tggcgtttgc gggcaagaac gcccgaccgg tcaaaaggcc
     1261 ggaattcccc gaccagaatg gaaggaccgc cgatgctcag caattcggtg aataccaacg
     1321 tgggcgcgct ggttgcgctc cagaacctca acgcgaccaa catggaactg tcgcagaccc
     1381 aatcccgcat caacaccggc aagaaggtcg ccaccgcgaa ggacaacggg gccatctggg
     1441 ctatcgccca gacgcaacgg gggaattcgc aggccctgaa cgccgtgcgg gactctctcg
     1501 accgcggccg gtccaccatc gatgtggccg tggcggcggg ggaatcggtc tccgacctgt
     1561 tgaatcagat gaaggaaaag gccctggcgg cgtcggattc cacactcgac tccaccagcc
     1621 gttccgcgct gaacgaggac ttcaaggccc tgcgcgacca gatcgccaag gctgtgtcga
     1681 acgccgactt caatgggacc aatctcatta agaccggcgc cacggcgctc gcggccctcg
     1741 ccaacgcgga cggatcgtcc aagctcaccg tggccgcaga agtcatggcg ctgggcggtt
     1801 cgatcgtgac cgtcgctgcg gcgggctcga tcggcacggc gaccctggcc acggccatga
     1861 tctccacggt caacacctcc atcgccaaca cgaacgcggc gctcgcccgc cttggcacca
     1921 agtccaaggc gttcgatctg cacaccacct tcatcggcaa gctgcaggac agcctcgatg
     1981 cgggggtggg caatctggtg gacgcggatc tcgccaagga aagcgccaag ctccaggctc
     2041 tgcagaccaa gcagcagctc ggggtccagg ccctctccat cgcgaattcg tcgacccaga
     2101 cgcttctgtc gttgttccag taaggccgtg acgccgggcg gaggcaccct ccgcccggca
     2161 gtcttttgcc gggccacccg gcagaagttg ctcaccatgc ccacggcccg gcagaaatta
     2221 ccgggccaaa acgtccctaa cagacctata atccattaaa aacagcaact tgctgaaacg
     2281 ccccggcccg gccaaattgg cccggcgatt gcttcccacc ccatcgggca aaaggccccc
     2341 ggccgtcaaa acgacgccgg acacctggaa agggaactat cgtcatggcg ctgaacagcg
     2401 ttaacacgaa caccggcgcg cttgtcgccc tgcagaacct gaacgccacg aactcggagc
     2461 tgaacgtcgt tcagggccgg atcaacacgg gcaagaaggt cgcttccgcc aaggacaacg
     2521 gcgccatctg ggccatcgcc cagacccagc gcggcacgtc ccaggccctg aacgccgtca
     2581 aggattcgct ccagcgcggt caatccacca tcgacgtcgc gatcgccgcc ggcgagtccg
     2641 tttccgatct gctcgtgcag atgaaggaga aggccctggc cgcgtcggac acgacgctcg
     2701 acaccgccag ccgttcggcc ctcagcgaag acttcaaggc gctgcgtgac cagatcgcca
     2761 aggcggtgag caacgccgac ttcaacggct cgaacatgat caaggcgtcg ggcgccaccg
     2821 tgacggctct ggccaacgcg gacggcacct cgaagatcac ggtcgcggcg cagtccctcg
     2881 ctctgggcgg cgccaacgtg acggtcgccg ccacggcgtc gctgagcacg gccaccatcg
     2941 cggcggccat ggtcaccacc gtcagcacct cgatcaccaa cgtcggcgcg gccctggcca
     3001 agctcggcac gggttcgaag tcgctggaag cccacctgac cttcgtcggc aagctgcagg
     3061 acagcctgga cgccggcgtc ggcaacctgg tggacgcgga tctggccaag gaaagcgcca
     3121 agctgcaggc cctgcaaacc aagcagcagc tcggcgtgca ggccctggcg atcgccaaca
     3181 cctcgacgtc ggccctgctc gggctgttcc gctaacaggc aagcgcgggg gcgggcgtct
     3241 gacgtccgtc cccggttgcg acggcgaggg ccgccaggcc ttcgccgttc gccaacagga
     3301 gggcggccgg tcgcaaaggg ccggcgccag gagacatgaa aaccaacgtc acacccgcct
     3361 tgtcgatcca ggatcgggga gcctttccgg cttcgccgtt cgcgggtccc gacgccgtca
     3421 agccaccgcc ggagactccg cgtcagaacg ccgacctgcg cctggtcatc gaggaagacc
     3481 agacctcggg aaccttcgtc tacaagaccc tcgaccgccg gacgggcgag gtcgtcaagc
     3541 aactgccccg tgaggaggtt gtgcgactga aggatgcacc cggctacgcg cccggcgccg
     3601 tgatcgacgc ccagtcatag cggaccgccg ctgcgcggct ggcccgaccg agacttgtca
     3661 ccgacgtcgc gccgcgacgg cggccttcat ggtttaccgc cgttaaccat aagcgaacgt
     3721 tctcccgcct agcgttgcgc ggggtgtctc gccgagtcgc ctcacgtctt gatgcgccgc
     3781 gcgaggagaa gccccatgtc gatcagcgtc cataccaacc agtccgcgct ggttgcgctg
     3841 cagaacctca accgcaccaa cgacgagctc gccggggcgc agaaccgcat cagcaccggc
     3901 ctgaaggtgc aggggcccaa ggacaacgcc tcggtctggg gcatcgccca gtcgcagcgg
     3961 gccgacgtcg gcgccctcgg ggcggtcaag atgagcctcg accgggccca gtcgatctcc
     4021 gacgtggcga tctcggccgg cgaatcgatt tccgacctgt tgctctcgat gaaggagaag
     4081 gtcaccgcgg cgatggaccc ctcgctggac tcggtctcgc ggacggcgct ggacgcggac
     4141 tacaagtccc tgcttcgcca gatcacccag gtggtcaacg acgccgagtt cgacggggcc
     4201 aacatgctgg acggctcgct cggcgccggc atccgtttcc tggccaacgc cgacgccaac
     4261 tcgttcatca ccctgacggc caagaacatg tcgctgggcg gatcgatcat cacccttggc
     4321 gcggccagtt cgatcaccac cgcgaccctg gccaccacca ttcagggcct gctgagcacc
     4381 tcgatcgcca acgtcaacca ggcgctcggc gacatgggcg ctcagtcgcg ccaggtggga
     4441 tcgcacaaca ccttcgtctc caagctgacc gacgtgctca actcgggggt cggcaacctg
     4501 gtcgacgccg acctggccaa ggaaagcgcg cgcctgcagg ccctgcaggt ccagcagcag
     4561 ctgggcgccc aggcgctgtc gatcgccaac tcggcgccgc agatcgtgct gtcgctcttc
     4621 aagggttgat cgaggcgggc gcgaggctgc gctatagtcg cgtatcgacg gccgttggag
     4681 gggtggcatg atcgagcttc gcgaccttgt tccagaggac gaggatcgcc tgtttcagtg
     4741 gcgcggcgaa cccgatgtcg cccgctggat gtcggacgcc gacgtgtccg gccgcgaggc
     4801 tcatcgggcc tggttcgacc agctgcgggt cgatccggac ctgcggggct ggctgatcga
     4861 aagcggcggc cgtccctccg gcttgctgac cctgaccgga ctgaccggtc accatcgtcg
     4921 ggccgcctgg aactggttca tcggtgatcg cgaggcccgg ggccggggcg tcggtcgggc
     4981 ggcccaggtg ctcggcctcg accaggcttt cggcgtgctg ggcctgcaca aggtctgggc
     5041 cgaggtcatg gccgacaacg acgcggccat gaaggtccag acgggggccg ggtttcgccg
     5101 cgagggctat ctcaggggcc atgtgctcaa ggacggccgg ccccgcgacg tggtcatgct
     5161 ggggatcctg gccgaggaat gggcggtgct ggcgccggga gcgcggcgcc tgctggccga
     5221 cgcgggcctg atcgccgcct gagcgcgcct taacaccccg tcagcccttg tctggcaagg
     5281 tcctcctgcg agactccgcc aggattcaaa tggccgtcac cttcgatagc agcatcctgc
     5341 tgaactatta ccagtcccgg tcgggtgcga ccgcgttgac tggcggcggc gctgcgtctt
     5401 cgggcgggac gacgacgaag tacgcgccga ccccgccctg ggcgaccaac tcgggcgtcg
     5461 cgcgggccag tgatctggtc aagtcggcca tgtccgggcg gcggttcatc gacgagggct
     5521 cggccaagct cgatctcgag ggcgccagcg cggactacaa gaagctgttc gccctctacc
     5581 aggggctcaa taccctctac ggcctgggcg agcgcctgca ggccaagggc gtgtcgacca
     5641 cggagaagaa ccgcatcgcc gagatcttca cccgcggtct gggcgaaatc tcgacctaca
     5701 ccgacaaggc caggctcgac cagatgcgcc tgacccgcgg cgacacgatg atctcggaca
     5761 agtcggcggt cggcgttccg cgggcgaagt accagtacct gaccggcaag gtctattcgg
     5821 gcgcccaggg ggacgcggtg ccggccttcg ccggagatgt cgccttcacc ctgacggtca
     5881 agcgcggcac ggtcacccat tcgatcgaca tcaacctcgc cgacatgggg gcccaggacc
     5941 ggacgatggg ggcggtgacg tcgttcatca acggcaagct cgaggccgag gggctgcaga
     6001 cccgtttcag cgtggtgcgc acgcccggcg agccgcgcaa ggtgacgacc ggcggcacca
     6061 ccgtgaccct gccggccgtg gccgacgact tcgccttcag gatcaccggg gattcgaccg
     6121 agcaggcgac cttctcggcg gccaccacca agccggccgt ctatgtcacc acctatgccg
     6181 gcaatcccga cccggacaag gacatcaaga ccgacgacgg ggtgttcgag accagcctgg
     6241 tcaaggtcga cgggaccggc atcggaaccg agggcgcgcg ggtgttctcc tcgacgctcg
     6301 agggcacgat cgagtcggtg cgcacctcgc gggtcggggc tgacggcgcg ctgtacgtgc
     6361 tcgccgacgt caacacctcg gtcgacggcc agacggtcaa gggcgagcgc gacgtggccc
     6421 tgctgaagta cgacagcaca ggccagctga tgtacgcgcg caccctcggc gcggccgagg
     6481 acgcccaggg cctggcgctg acggtggccg atgacggccg tgtggccatc gctggcaagg
     6541 tcaccggcgc gctggcgggc gcgacggacg ggccgatcaa ctccggcgcc gacaccggcc
     6601 tgagcgacag cttcgtcacg gtgttcgacg cgaagggcga cgaggcctgg accgcgcggc
     6661 gtggcgcgct gaaggacgac gaggccacgg ccgtcgcgtt tggcgagggg gggctggtct
     6721 atgtcgccgg ccggaccaaa tcgacccagc cgggcggcgg cgcggccctg ggcgactggg
     6781 acggctacct gacggctttc gcggccgacg ccaagggcgt gcccagggcg ctgttcacca
     6841 accagttcgg cacggccggg tcggacaccg tcagcggcat cgtcgtcgac ggcgcgaccg
     6901 tcaccctggc cggcgtcgaa agcggtcgcg gcgtgctgcg cagcttctcg gtcacgccga
     6961 caaccacgac gacggaaaag atcgtcaacg acggcgtgac cacggtcaat gtcacgacca
     7021 ccaccaacgg cgcgtcgaac accaccagca atgtctacgg cgcgcccgat ggctcgggaa
     7081 cgagctctac gacgaccacg gtccacacct cggcggccag cgcggtggcc ggggccacgc
     7141 gcgacctcgg cgacctgctg ggcgggcaga tcgccggcat cggcctggac ggcggtgacc
     7201 tctgggtggc cggcacgacc cgcaacgccg ccctgtcggt ggccgggacc agccatgcct
     7261 acaatggcgg gttcgatgcc ttcgcggcgc gcatttcagc cgatctttcc agcgcttcgc
     7321 aggaccagct cacctatttt ggcggctcga ccgacgacac ggtgacggcc atggccgtgg
     7381 ccgacggcaa ggtctgggtc gccggccggg cggcggccga ccagcccggg gcgacggcca
     7441 tcgcgacgaa ggacggctat ctcgcccagc ttgacgtcgg cgccggcacg gcgacggtgc
     7501 agcggctgac cggcaaggac ggcatggcca ccgcgacctc gatcgccatc gaccagagcg
     7561 gcgccagcgc cctcgacagg ctcgggctgc ccaaggggac gctgctctac gccgactcgg
     7621 ccaggatcgc ctcggccacg gcggcgcggg caggggacac cttccagatc cgcacgtcgg
     7681 agggcggacg gctggccacg gtcaccctcg aagcgggcga taccctcgac agcctggcgg
     7741 tcaaggtgcg ccgggcggcc ggcttcaagg ccaggatcga ggtggtcagc gacggcgatt
     7801 tccggcggct gaagatcact ccggccacgg agggcgcgac ggtcgaggtt ctgcccggca
     7861 agggcggcgc cgacgccctc gaggcgatcg gcctgaccgc gggcgtgatc cgcaacaccg
     7921 tcatcgacaa ggatggcaag agcgtttcgg ccgatggcgg cgggccggta tttggcctgg
     7981 gcctgccggc ggagctggac gtcagcaccg aggccggggt gaagaacgcc ctggccgtgc
     8041 tctccaagtc gctcggcaag atccgcgacg cctaccgcca gctcgaggcg gcggcccggc
     8101 cccagacctc ggcggccagg tcggcgaacg ggccggttcc cgcctatctg accggccaga
     8161 tcgccaacta ccaggccgcg cttgatcgtt tgaccggcgg cggctgacgc ggaaccggtc
     8221 acgcgggctt gaaagcgccc ccaccgcaag atagcgattg cggccatgga atatctcaag
     8281 gaccgccgga tcgtcctggc catcggcggc gcggtcgcgc tgctgctggg catcatcttc
     8341 gcggcgacga tcatgggacg cgacaagggg ccggacaagc cgccgcctgc ggccaccggc
     8401 ggcctggtgg tggagaccgg gcgcgacgac gacacccgcc tcgatcctgc cc
//
LOCUS       DAORNX010000074         8366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6288_length_8366_cov_8.118518, whole genome shotgun sequence.
ACCESSION   DAORNX010000074 DAORNX010000000
VERSION     DAORNX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_8366_cov_8.118518"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..626
                     /locus_tag="PLO65_04860"
     CDS             12..626
                     /locus_tag="PLO65_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRD27612.1"
                     /translation="MDKAVPGDLVREILVAAQRAPSGGNLQPWRVHALAGAPLEALKA
                     RVAANPAGEEPEYDVYPPNLWDPFRTRRFQCGEDLYATIGIPREDKPARLRQLFRNTQ
                     FFGAPVGVLFSLDRKLGPPQWADVGMYMQTVMLLAVERGLATCAQEFWARYPKTVAEH
                     IALPDDHMLFSGMALGWPDDTAPINSLRTRRDPFEVWCAMEGFE"
     gene            complement(736..1728)
                     /locus_tag="PLO65_04865"
     CDS             complement(736..1728)
                     /locus_tag="PLO65_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRD27613.1"
                     /translation="MTFDFDAYVTAVIFDRSGRTLFALGDGTVRAVAGDGFVTVEAHN
                     GAVLSACPHPSGLGVVTGGDDGRVVWTRLEGGEIAASLVAEVKGRWIDAVAASAESGL
                     IAFTAGRELHVRDSKDPAFSRVFVHEKSAADVAFDPRGRRLAVATYNGAALWYARIAD
                     QKPQLLKWAGSHVRVLWSPDGRFLMSAMQENQLHGWKVADEKNLRMGGYPAKVKSLAF
                     LAGGLMMATSGANGAVIWPFAGATGPMGKEAAEIGYDQAAMVVQVAGTPSGHGLAAGL
                     DDGRVWVADLRSGGIEHLKAEKGAAISALAISPDGSRVAWGDEDGRAGVVETGL"
     gene            1884..2333
                     /locus_tag="PLO65_04870"
     CDS             1884..2333
                     /locus_tag="PLO65_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRD27614.1"
                     /translation="MAAKLGGGGGGSKYDLGQNSDINVTPFVDIMLVLLIIFMVAAPM
                     ATVSIALDLPPAVPPPPGEKPKKPAFISIQNSGAIFVGDVQSSLANLNIDVEQAMKNE
                     GVTSNFTEERVFVRADGDVMYEQFMAVLNVLQAYGYFKIGLINEDIE"
     gene            complement(2488..3552)
                     /locus_tag="PLO65_04875"
     CDS             complement(2488..3552)
                     /locus_tag="PLO65_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD27615.1"
                     /translation="MTDASPARIPVTVLTGFLGAGKTTLLNRILTEDHGKRYAVIVNE
                     FGEIGIDNDLVVGADEEVFEMNNGCVCCTVRGDLIRVVSGLMKRKGGFDAIIVETTGL
                     ADPGPVAQTFFVDDDVRARTKLDSVTTVVDAKHLPLRLADSREAVEQIAFADQILLNK
                     TDLVTPAELKAVEAQIRRINPLAPIHHSQRSAVPLDAILGRGGFDLDRITELEPEFLN
                     PAHGEAGHVHDESCDHDHHHHDHDHGHHDHAAHDHVAEAGIRGVSLTSERPLDSIKVT
                     NWLNQLLADKGPDILRAKGILDVKGEDRRLVFQAVHMILEGDFQREWKSGEPRYSRMV
                     FIGRDLDEAALKAGFEACVA"
     gene            complement(3571..4218)
                     /locus_tag="PLO65_04880"
     CDS             complement(3571..4218)
                     /locus_tag="PLO65_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27616.1"
                     /translation="MISPFFLSFAAAGFGVALVHVAIPTHWLPFVLVGRAQGWRLPRV
                     LGAASAAAVGHILTTSVAGVLILMAGRFVEQWLQGVLHYAAAALLFGFGLFYLARALQ
                     RRVALAGMTDQTLAPPARVGDRAAWWGVVGALALSPGEVLLSFYLTGDTHGWPGLALL
                     SAMFLIGTLAGMVALISVTWAGMAHFRMDRLARYESAILGAVLVTLGVAVVVFRA"
     gene            4375..4992
                     /gene="rplU"
                     /locus_tag="PLO65_04885"
     CDS             4375..4992
                     /gene="rplU"
                     /locus_tag="PLO65_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRD27617.1"
                     /translation="MYAVIKTGGKQYRVKAGDLLVVEKLAGEPGADVAFGDVLMLGDG
                     DAVTVGAPLVDGATVNATLIETRKGEKVKIFKKIRRQGYRRTKGHRQHETVLRVTSVA
                     GGGKTDKWDGEVSLTTKRELDARARGLAFQPLGGATPKTEKAAPAAPAAKAPAPKAEA
                     APATEAKAAAPKKAAAPKAAKADGEAAPAKKAAARKPAAKKDSDA"
     gene            5025..5297
                     /gene="rpmA"
                     /locus_tag="PLO65_04890"
     CDS             5025..5297
                     /gene="rpmA"
                     /locus_tag="PLO65_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRD27618.1"
                     /translation="MAHKKSGGSSRNGRDSAGRRLGVKKFGGEKVLAGNIIIRQRGTK
                     FWPGMNVGIGKDHTLFALVSGAVKFVTKRNNRTYATVVPAEAQAAE"
     gene            5435..5968
                     /locus_tag="PLO65_04895"
     CDS             5435..5968
                     /locus_tag="PLO65_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27619.1"
                     /translation="MGLIDRTPRIETRRLTLRAPRPADAPVLAALLDDFDIARMTSSM
                     PFPYGLADAETYLAHCAAADPRTDASFLIEHDDEGPIGGLGFDPSPEGGIEVGYWIGK
                     PWWGRGFASEALVGAMTWAARDWRRRLVVAGHFRDNPASGRVLCRAGFLYTGRVELRH
                     SRSRGEPVATRMMVWLA"
     gene            complement(5972..6604)
                     /locus_tag="PLO65_04900"
     CDS             complement(5972..6604)
                     /locus_tag="PLO65_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRD27620.1"
                     /translation="MRAGFRLLTVLIALTLAPAASMAARPALWPGDMTLGNPKAKIVV
                     VEYASLSCPHCARFHKEIFPAFRKKYIDTGKVQFVWREFVTSPAEVAVPATMLARCAG
                     KDRFFQVVDRVFAAQGEMYEIGTVSGVHRILRREAAAVGIDGPAYDACVNDQAGFDAL
                     KARIDRANSEGKVDGTPTFDVNGKRVVAPLGQEMDLATLEAAIKAAGKAK"
     gene            6721..7779
                     /gene="obgE"
                     /locus_tag="PLO65_04905"
     CDS             6721..7779
                     /gene="obgE"
                     /locus_tag="PLO65_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRD27621.1"
                     /translation="MKFLDQCKIYVRSGDGGGGAVSFRREKYIEYGGPDGGDGGRGGD
                     VWIEAVEGLNTLIDYRYQQHFKAGTGVHGMGRNRHGAAGEDIVLKVPVGTQVLEEDKE
                     TLVADLDAPGKRVLLARGGNGGWGNDRFKGPVNQAPKHANPGQPGEERWLWFRLKLIA
                     DAGLVGLPNAGKSTFLAAVSAARPKIADYPFTTLTPNLGIVDLSSEERFVIADIPGLI
                     EGASEGAGLGTRFLGHVERAGALIHLVDATQEDVVGAWRTVRGELEAYGEGLADRPEI
                     LALNKVDAITAEERAEKAAALEAAAGKPPMIISGVSGEGVPDLLRAAYRAVKRHRDAE
                     TAAAAGEATAEPAGWSPV"
     gene            7794..8228
                     /locus_tag="PLO65_04910"
     CDS             7794..8228
                     /locus_tag="PLO65_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27622.1"
                     /translation="MLVSILSAFALMLAEAPATSPAPPPTVPATPAAAPAAVDPVTAE
                     INGLLEPYKGKPGDALRGKLGLSAGVRQASDGEVVFWVLSIEQETVCGMDPATGAMRC
                     LRGDPFQCRLAIAFDKQSAVKAWAVSGDPAVCRGFIGKLKGG"
     gene            8228..>8366
                     /locus_tag="PLO65_04915"
     CDS             8228..>8366
                     /locus_tag="PLO65_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRD27623.1"
                     /translation="MTGSSSGLTSARRVVIKVGSALVVEAQTGLPARDWLAAFAADAA
                     RL"
BASE COUNT         1332 a         2951 c         2865 g         1218 t
ORIGIN      
        1 tccgcgcctt cctggacaag gccgttccgg gcgatctggt gcgcgagatc ctggtcgccg
       61 cccagcgcgc gccgtccggc ggcaacctgc agccctggcg ggtccacgcc ctggccggcg
      121 cgccgctgga ggcgctcaag gccagggtgg ccgccaatcc ggccggcgag gaaccggaat
      181 acgatgtcta tccgcccaat ctgtgggacc cgttccgcac ccggcggttc cagtgcggcg
      241 aggacctcta cgcgaccatc ggcatcccgc gcgaggacaa gccggcccgg ctgcggcagc
      301 tgttccgcaa cacccagttc ttcggcgctc ccgtgggcgt gctcttcagc ctcgaccgca
      361 agctcggccc gccgcagtgg gccgacgtgg gcatgtacat gcagacggtc atgctgctgg
      421 ccgtcgagcg cggcctggcc acctgcgccc aggaattctg ggcccgctac cccaagacgg
      481 tggcggaaca catcgccctg cccgacgacc acatgctgtt ttccggcatg gccctgggct
      541 ggcccgacga caccgccccg atcaacagcc tgcggacgcg ccgggacccg ttcgaggtct
      601 ggtgtgcgat ggaagggttt gaatagcgtc cctgcgtcct tcgacatggc cgctgcgcgg
      661 cctgctcagg atgacgtaac tcggtgcacg tcatggtgag cagcgccgca ggcgcgtgtc
      721 gaaccacgcg aactgctaca gccccgtctc cacaacgccc gcccggccgt cctcgtcgcc
      781 ccaggcgacg cgcgagccgt ccggactgat cgccagggcg ctgatcgccg cgcccttttc
      841 cgccttcagg tgctcgatcc cgccgctcct gaggtcggcg acccagacgc ggccgtcgtc
      901 gagaccggcg gccagcccat ggcccgaggg ggtcccggcc acctgcacga ccatcgccgc
      961 ctggtcatag ccgatctcgg cggcctcctt gcccatcggg ccggtggcgc cggcgaacgg
     1021 ccagatcacc gcgccattgg cgcccgacgt cgccatcatc agcccgccgg ccaggaaggc
     1081 caggctcttc accttggccg gatagccgcc catgcgcagg ttcttctcgt cggccacctt
     1141 ccagccgtgc agctggtttt cctgcatggc gctcatcagg aaccggccgt cggggctcca
     1201 cagcacgcgc acatggctgc cggcccattt gagcagttgc ggcttctggt cggcgatcct
     1261 cgcgtaccac agggccgcgc cgttgtaggt ggccaccgcc aggcgccgcc ccctggggtc
     1321 gaaggcgaca tcggcggccg acttctcgtg gacgaacacg cggctgaagg ccgggtcctt
     1381 cgaatcccgc acatgcaact cgcggccggc ggtgaaggcg atcaggcccg attcggcgct
     1441 ggccgcgacc gcgtcgatcc agcggccctt gacctcggcg accaggctgg cggcgatctc
     1501 gccgccttcc agacgggtcc agaccacccg cccgtcgtcg ccgccggtga cgaccccgag
     1561 gccggacggg tgcggacagg cggacaggac cgccccattg tgggcctcga ccgtgacgaa
     1621 gccgtccccg gccacggccc gcacggttcc gtcgcccagg gcgaacaatg ttcggccgga
     1681 gcggtcgaaa atgacggcgg tcacataggc gtcgaaatcg aaagtcatga tcgcgcgttt
     1741 atagcccagg cccggccttg cccagcggcg gatcgttttc gctcgccagg cgagacgggc
     1801 tttacaagcg gcttcaaccg cgtaatgggt tcgaacgaat gacagcgggc cgcagacgtc
     1861 ggcgcgcgct agggagtgtc cgaatggcgg cgaaactggg cgggggcggc ggcggctcca
     1921 agtacgatct gggacagaac agcgacatca acgtcacgcc gttcgtcgac atcatgctcg
     1981 tgctgctgat catcttcatg gtggccgcgc cgatggcgac ggtgtccatc gcgctcgacc
     2041 tgccgccggc ggttccgccg cctccgggcg agaagccgaa gaagccggcc ttcatctcga
     2101 tccagaacag cggcgccatc ttcgtcggcg acgtgcagtc gagcctggcc aacctcaata
     2161 tcgacgtcga gcaggcgatg aagaacgagg gcgtgaccag caacttcacc gaagagcgcg
     2221 tgttcgtccg cgccgatggc gacgtgatgt acgagcagtt catggccgtg ctgaacgtcc
     2281 tgcaggccta cggctacttc aagatcggcc tgatcaacga agacatcgaa tagtcgggcg
     2341 tcatcgccag acatgcgaag ggccggtgga aacaccggcc cttttgcttt gcggatctgg
     2401 ggacatgtac cgccttcggt acgtgtcccc tgttcacgag gttcggatgg gggacacgta
     2461 cctgcggtac atgtccccgt cagcgcccta ggccacgcac gcctcgaaac cggccttcag
     2521 cgccgcttcg tccagatccc gcccgatgaa caccatccgg ctgtagcgcg gttcgccgga
     2581 cttccactcg cgctggaagt cgccctcgag gatcatgtgc acggcctgga acaccagtcg
     2641 gcggtcttcg cccttcacgt cgaggatgcc cttggcgcgc aggatgtcgg gacccttgtc
     2701 ggccagcagc tggttcagcc agttggtcac cttgatgctg tccagcggcc gctccgaggt
     2761 cagcgacacg ccgcggatgc cggcctcggc cacgtgatca tgggcggcat gatcgtggtg
     2821 gccgtgatcg tggtcgtgat gatgatggtc gtgatcgcac gactcgtcgt gcacgtgacc
     2881 ggcctcgccg tgcgcgggat tgaggaattc cggctccagt tcggtgatcc ggtccaggtc
     2941 gaacccgccg cgaccgagga tcgcgtcgag cggcacggcc gagcgctggc tgtggtggat
     3001 cggcgccagc gggttgatcc ggcggatctg ggcctcgacg gccttcagct cggccggggt
     3061 caccaggtcg gtcttgttga gcaggatctg gtcggcgaag gcgatctgct cgaccgcctc
     3121 gcggctgtcg gccagccgca gcggcaggtg cttggcgtcg accacggtgg tcaccgagtc
     3181 cagcttcgtg cgggcgcgca cgtcgtcgtc gacgaagaag gtctgggcca ccgggcctgg
     3241 atcggcgagg cccgtggtct cgacgatgat cgcgtcgaag ccgcccttgc gcttcatcag
     3301 gcccgagacc acgcggatca ggtcgccgcg cacggtgcag cagacgcagc cgttgttcat
     3361 ctcgaacact tcctcgtcgg cgccgacgac caggtcgttg tcgatgccga tctcgccgaa
     3421 ctcgttgacg atcacggcgt agcgcttgcc gtggtcctcg gtgaggatcc ggttgagcag
     3481 ggtggtcttg ccggcgccga ggaagccggt gaggacggtg acgggaatgc gggcggggga
     3541 agcgtcggtc atggcggcta gataggcggc tcaggccctg aaaaccacca ccgcgacgcc
     3601 cagcgtcacg agaaccgcgc cgaggatcgc cgattcgtag cgcgccaggc ggtccatgcg
     3661 gaaatgggcc atgccggccc aggtcacgct gatcagcgcg accatcccgg ccagggtgcc
     3721 gatcaggaac atcgcgctga gcagcgccag gcctggccag ccgtgcgtgt cgcccgtgag
     3781 atagaaggac agcagcacct cgcccggcga cagcgccagg gcgccgacga cgccccacca
     3841 ggcggcccgg tcgccgacgc gcgccggcgg cgccagggtc tggtcggtca tccccgccag
     3901 ggccacccgc cgctgcagcg cgcgcgccag gtagaacagt ccgaaaccga acagcagggc
     3961 ggcggcggca tagtgcagga cgccctgcag ccactgctcg acgaaccggc cggccatcag
     4021 gatcagcacg ccggcgaccg aggtggtcag gatgtgtccg accgccgcgg ccgacgccgc
     4081 gcccagcacc cggggcagcc gccagccctg cgcccggccg accagcacga acggcaacca
     4141 gtgcgtggga atggccacat gcacgagggc gacgccaaag ccggccgccg cgaaggagag
     4201 gaagaagggc gagatcaccc gacgggctat aacctgttac aaagtaacat gccagtgggg
     4261 acggcgcggc gttgactcgg aaagcgccgg ctgtataaga ccgctccctc gcccgcggga
     4321 cacctcgcgg gcgcgatcta ttttgcgaac tctagatttt tcaaggcgca cacgatgtac
     4381 gcggtgatca agaccggcgg caagcagtac cgggtcaagg cgggcgacct gctcgtcgtc
     4441 gagaagctgg ccggcgagcc gggcgcggac gtcgccttcg gcgacgtgct gatgctcggt
     4501 gacggcgacg cggtgaccgt gggcgctccg ctggtggacg gcgcgaccgt caacgccacc
     4561 ctgatcgaaa cccgcaaggg cgagaaggtg aagatcttca agaagatccg ccgtcagggc
     4621 tatcgtcgca ccaagggtca tcgccagcat gagaccgtgc tgcgcgtcac ctcggtggcc
     4681 ggcggcggca agaccgacaa gtgggacggc gaggtcagcc tgacgaccaa gcgcgagctc
     4741 gacgcccgcg cccgcggcct ggccttccag cccctcggcg gcgccacgcc gaagacggaa
     4801 aaggccgctc cggccgctcc cgccgccaag gcccccgcgc ccaaggccga agccgctccg
     4861 gcgaccgaag ccaaggccgc cgcgccgaag aaggccgccg cccccaaggc cgccaaggcc
     4921 gacggtgaag ccgcccccgc caagaaggcc gctgccagga agccggccgc caagaaggac
     4981 agcgacgcct gatcggcggc gctgacagag agtaggagag caccatggct cacaagaaat
     5041 caggcggctc ctcgcgcaac ggccgcgact ccgccggccg ccgtctcggc gtcaagaagt
     5101 tcggcggcga gaaggtcctc gccggcaaca tcatcattcg tcagcgcggc accaagttct
     5161 ggccgggcat gaatgtcggc atcggcaagg atcacaccct gttcgcgctg gtatcgggcg
     5221 cggtcaagtt cgtgaccaag cgcaacaacc gcacctatgc gacggtcgtt ccggccgagg
     5281 cccaggccgc cgagtaggcg atctggaaag acctcctccg gatcgcccga cacaggcgat
     5341 ccggacatga aattcctcaa gcggggagtc cggacgccgg gctccccgct tttgcatttc
     5401 gaggcccccg cagaggggcc gggaggacca gccgatgggc ctgatcgaca ggacgccacg
     5461 gatcgagacg agacggctga ccctcagggc gccccggcca gccgacgcgc cggtgctggc
     5521 cgccttgctc gacgacttcg acatcgcgcg catgacctcg tccatgccct ttccctacgg
     5581 cctggccgac gcggagacct acctggccca ttgcgcggcg gccgacccac gcaccgacgc
     5641 gtcgtttctg atcgagcacg acgacgaggg cccgatcgga ggcctcgggt tcgacccctc
     5701 gcccgagggc gggatcgagg tcggctactg gatcggcaaa ccctggtggg gtcgcggctt
     5761 cgccagcgaa gcgctggtcg gggccatgac ctgggccgcc cgcgactggc gcaggcgcct
     5821 ggtcgtcgcc ggtcatttcc gggacaatcc ggcgtccggc cgggtgctct gccgcgccgg
     5881 attcctctac accggccggg tcgagttgcg gcacagccga tcgcgcggcg aaccggtcgc
     5941 gacccgcatg atggtctggc tggcgtgagg cctacttcgc ctttcccgcc gccttgatcg
     6001 ccgcctcgag ggtcgccagg tccatctcct ggccgagcgg cgcgacgacc cgcttgccgt
     6061 tgacgtcgaa ggtcggggtg ccgtcgacct tgccctcgct gttggcccgg tcgatgcggg
     6121 ccttgagcgc gtcgaagccg gcctggtcgt tgacgcaggc gtcgtaggcg gggccgtcga
     6181 tgcccaccgc cgccgcctca cgccgcagga tgcggtggac cccgctgacg gtgccgatct
     6241 cgtacatctc gccctgggcg gcgaacaccc ggtcgacgac ctggaagaac cggtccttgc
     6301 cggcgcagcg ggccagcatg gtcgcgggca cggccacctc ggccgggctg gtgacgaact
     6361 cgcgccagac gaactgcacc ttgccggtgt cgatgtactt cttgcggaag gccgggaaga
     6421 tctccttgtg gaagcgggcg cagtgcgggc agctcagcga ggcgtactcg acgaccacga
     6481 tcttcgcctt cggattgccc agcgtcatgt cgccgggcca cagggccggg cgggccgcca
     6541 tcgaggccgc cggcgccagc gtcagggcga tcagcacggt cagcagtcga aatccggcgc
     6601 gcatggcctt cccccgttcg gtgtccagat cgctcctatg aggccatccg ggcatctttg
     6661 cgaccgttcc cgcggccgcc tgcccttttc tggaccccgc ccttggccca taagggcgcg
     6721 atgaaatttc tcgaccagtg caagatctat gtccgctccg gcgatggcgg cggcggcgcg
     6781 gtctcgttcc ggcgcgaaaa atacatcgag tacggcgggc cggacggggg cgacggcggg
     6841 cgcggcggcg atgtctggat cgaggccgtc gaggggctga acaccctcat cgactaccgc
     6901 taccagcagc atttcaaggc cggcaccggc gtgcacggca tgggccgcaa ccgccacggc
     6961 gcggccggcg aggacatcgt cctcaaggtg ccggtcggga cccaggtgct cgaggaagac
     7021 aaggagacgc tggtcgccga cctcgacgcc cccgggaaac gcgtgctcct ggccaggggc
     7081 ggcaacggcg gctggggcaa cgaccgcttc aaggggccgg tcaaccaggc ccccaagcat
     7141 gccaaccccg gccagccggg ggaggagcgc tggctgtggt tccggctcaa gctgatcgcc
     7201 gacgccgggc tggtcggcct gcccaacgcc ggcaagtcga ccttcctggc cgccgtcagc
     7261 gcggcccggc cgaagatcgc cgactatccg ttcaccaccc tgacgcccaa cctcggcatc
     7321 gtcgacctgt cgtcggaaga acgcttcgtc atcgccgaca tcccgggcct gatcgagggg
     7381 gcgtccgagg gcgccggcct gggcacccgg ttcctcggcc atgtcgagcg ggccggcgcc
     7441 ctgatccacc tggtggacgc cacccaggag gacgtggtcg gcgcctggcg caccgtgcgc
     7501 ggcgaactgg aggcctacgg cgagggcctg gccgaccggc cggagatact cgccctgaac
     7561 aaggtcgacg cgatcaccgc cgaggaacgg gctgaaaagg cggccgccct cgaggccgcg
     7621 gccggcaagc ctccgatgat catctcgggc gtgtccggag agggcgtgcc ggatctgctg
     7681 cgcgccgcct accgggcggt gaagcgccat cgtgacgccg agacggccgc cgcggccggc
     7741 gaggccacgg ccgaacccgc cggctggtca ccggtctagg tcaggggaac atcatgctgg
     7801 tctccattct gtccgccttc gccctgatgc tcgccgaggc ccccgccacg tcacccgctc
     7861 cgccgccgac cgttccggcg acgccagccg ccgcgccagc cgccgtcgac ccggtcaccg
     7921 ccgagatcaa tggcctgctc gagccctaca agggcaagcc gggcgacgca ttgcgcggca
     7981 agcttggcct gtcggcaggc gtgcgccagg ccagcgacgg cgaggtggtc ttctgggtgc
     8041 tgtcgatcga acaggagacc gtctgcggca tggacccggc caccggcgcc atgcgctgcc
     8101 tgcgcggcga cccgttccag tgccgactgg ccatcgcctt cgacaagcag agcgccgtca
     8161 aggcctgggc cgtcagcggc gacccggccg tctgccgcgg gttcatcggc aagctcaagg
     8221 gcggctgatg accggttcct cctcggggct gaccagcgcc cgccgcgtgg tgatcaaggt
     8281 cggctcggcg ctggtggtcg aggcccagac aggcctgccc gcccgcgact ggctggccgc
     8341 cttcgccgcc gacgccgccc gtctgc
//
LOCUS       DAORNX010000075         8228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6417_length_8228_cov_7.261104, whole genome shotgun sequence.
ACCESSION   DAORNX010000075 DAORNX010000000
VERSION     DAORNX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_8228_cov_7.261104"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO65_04920"
     CDS             complement(<1..216)
                     /locus_tag="PLO65_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27624.1"
                     /translation="MTSILPARFAPALAALALAAVLAGCGPEQPGPYVPPPGPPPPPP
                     ITLAPSLIQEASAWRGYMARAGAITPDF"
     assembly_gap    384..425
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(522..1457)
                     /locus_tag="PLO65_04925"
     CDS             complement(522..1457)
                     /locus_tag="PLO65_04925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRD27625.1"
                     /translation="MKLLAGNSNRTLATHIAEYLDIPLTRAQVRRFADNEVFVTIDEN
                     VRGEDVFVIQSTSFPANDNLMELLICIDALKRASGRRITAVMPYFGYARQDRKTGGRT
                     PISAKLVANLITRAGADRVLTMDLHAGQIQGFFDIPTDNLLPVPLLAQDIRSHYGKTQ
                     DLLIVSPDVGGVVRARALAKRIDADLAIVDKRRSGPGESEVMNIIGEVAGRRCILFDD
                     IVDSAGTLCNAAKALSDAGATEVSAYVTHGVLSGAAADRVAASVLKELVVTDSIELSD
                     PVKACKKIRAVSCAPLMGEAIRRIANEESVSKLFD"
     gene            1899..2240
                     /locus_tag="PLO65_04930"
     CDS             1899..2240
                     /locus_tag="PLO65_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27626.1"
                     /translation="MLVGLIGALALASTSAPPVDLDIAPGFSGVPCVPTRIIRVGADG
                     SLAPKGQSIQKSRVVPALGPVSGGQCERVNMTVDDGAPYGAVFDLISILRAAGFRQIA
                     LVDRPWEDAAP"
     gene            complement(2237..3241)
                     /gene="cobS"
                     /locus_tag="PLO65_04935"
     CDS             complement(2237..3241)
                     /gene="cobS"
                     /locus_tag="PLO65_04935"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HRD27627.1"
                     /translation="MADQTDTTPDPLLSLVPDKQVSIRDVFGVDSDMMVPAFSQRDPH
                     VPDLDEAYRFDPQTTLAIVAGLAFDRRVMIQGYHGTGKSTHIEQVCARLNWPMVRVNL
                     DSHVSRIDLVGKDAIVLKDGKQITEFREGILPWCLQRPMALVFDEYDAGRPDVMFVIQ
                     RVLEAGGKLTLLDQNRVIRANPYFRLFSTTNTIGLGDTTGLYHGTQQINQGQMDRWSI
                     VTTLNYLEHDAEAEIVLAKNPKYATPEGRRTLAAMVRVADMTRNAFMNGDISTVMSPR
                     TVITWAQNAEIFGGDLALAFRMTFLNKCDELERPTVAEFYQRAFGEDLPESAARVKVA
                     "
     gene            complement(3380..4036)
                     /locus_tag="PLO65_04940"
     CDS             complement(3380..4036)
                     /locus_tag="PLO65_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRD27628.1"
                     /translation="MANAFQYRPKFVDIRVRPPKPGEAEAEADVNALKPGERRCDHSD
                     CLNVATARAPKSRDMPGQFYHFCQSHAAEYNKQWNFFAGMTDEAITRAQAERATGERP
                     TWSFKASNRSREAAAQAKGPMGWNDPFSLFGFGRADRSGVDAPATRADGRTLGKLERN
                     ALADLDLPDTAEKALIRTRYTELVKKCHPDSNGGDRSAEHKLQRVLKAWKTLKKAGLA
                     "
     gene            4089..4361
                     /locus_tag="PLO65_04945"
     CDS             4089..4361
                     /locus_tag="PLO65_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRD27629.1"
                     /translation="MPSISDSIRIKLTDAFSPTRLEVVDDSARHAGHAGARPGGESHF
                     NVLIEAQAFAGLGKVARQRQVYGVLSELLAGPVHALSLKALAPGEG"
     gene            complement(4401..4766)
                     /locus_tag="PLO65_04950"
     CDS             complement(4401..4766)
                     /locus_tag="PLO65_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27630.1"
                     /translation="MTDLALDGRRILVVEDEMLVSMLLEDMLADFGCRVVGPAPTIAE
                     AMPLASGAEIDAAVLDLNLGGDPIFPVADALKARGVPFVFASGYGSGGLSEAHRGAPM
                     LQKPFRQADLERALRGLGL"
     gene            complement(4873..6171)
                     /locus_tag="PLO65_04955"
     CDS             complement(4873..6171)
                     /locus_tag="PLO65_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HRD27631.1"
                     /translation="MWALLLGLAPSLLILLGIGALISAAETSMTAASRGRMHQLERDG
                     DRAAARVNGMMGDQENMIGAILLSNNVVNIGASALTTSVLAAAFPGAIGVLASTVIMT
                     VLVVVFGEILPKTLAITRPEVVARFLSLPTFWLVRIFGPLARAAQWIVRQTLRPFGIN
                     LSMETDVLAAHEEIRGAVEYHHSEGVVESRDRRMLGGVLDLSEMDVSEVMVHRKSVEM
                     LDCALPARELVRQALDCNHSRLPLWRDDPDNIVGVLHVRDLVKVMAQTDVNLDALDVM
                     ALSRKPWFTPETTKLKDQLNAFLKKRNHFALVVDEYGGLQGVVSLEDILEEIVGEIDD
                     EHDATVEGLRVQPDGWVETAGTVTVRDLNRALDWDLPDDQAVTIAGLLIQEAQAIPEE
                     GETFTFFHHRFEVARRRRNQITELRISPRMDSEGGWIDSG"
     gene            complement(6174..7286)
                     /gene="aroB"
                     /locus_tag="PLO65_04960"
     CDS             complement(6174..7286)
                     /gene="aroB"
                     /locus_tag="PLO65_04960"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRD27632.1"
                     /translation="MIRSIPVALGGRSYEVLVGPGLIDRAGEHIAPLLKTRRTAIVTD
                     SNVGEHHGERLSVSLERAGVAVDMITVPAGEESKSFAGLAELSDRLLELRLDRGDLIV
                     AFGGGVVGDLAGFAAAIYKRGIDFIQVPTSLLAQVDSSVGGKTAIDTPRGKNLIGAFH
                     QPRLVLADLDVLCTLPAREMACGYAEVIKYGLLGDAGFFAWLEQHGRAVLDRDTDALA
                     HAVGLSVEMKAEIVAEDETEQGRRALLNLGHTFGHALEAETGYGETLKHGEAVGAGCG
                     LAFRFSAFLGQCDPAEAARVERALATAGLPAGMADVPGQPFDADRLIHHMGQDKKAQG
                     GALTFILARRIGEAFTAKGVDPDTLRRFLIEQGATS"
     gene            complement(7283..7831)
                     /locus_tag="PLO65_04965"
     CDS             complement(7283..7831)
                     /locus_tag="PLO65_04965"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRD27633.1"
                     /translation="MTADPRLRRKTIALVGLMGVGKSSVGRRLAAVLDLPFRDADAEV
                     EAAAGRTISEIFETFGEAAFRDGERRVIARLMDEGPHVLATGGGAFINDETRALIKQR
                     AVSVWLKADLALLARRIGRKDTRPLVRGKDPLEVLTALSVVRDPIYAQADITVETGDT
                     PHHEAVEAVLRALTAHLAGAEP"
     gene            7994..>8228
                     /locus_tag="PLO65_04970"
     CDS             7994..>8228
                     /locus_tag="PLO65_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27634.1"
                     /translation="MRPLVLAAALALLAAPAGAQVEVTQLAAPDYFSLGDRDSGLPAD
                     LWRGTSPAVARDVIPQIGVKLLSPAAARLARHLL"
BASE COUNT         1217 a         2673 c         2971 g         1325 t
ORIGIN      
        1 gaagtcaggc gtgatcgctc cggcgcgggc catgtagccg cgccaggcgc tggcttcctg
       61 gatcaggctg ggcgccaggg tgatcggcgg cggcggcggc gggccgggcg gcggcacgta
      121 ggggccgggc tgttcgggac cacagccggc gagcacggcg gcgagggcga gggcggcgag
      181 cgccggcgcg aaacgcgcgg gtaggatcga tgtcatgaac gcagcttccc cgtcacagcc
      241 caaccaagtc aatacaacgc gcgtgagcgg cgagaatgtg acgcaggggg tttaaggggg
      301 cctttacgat ggcgggccgc cggaggggcg agaggccagg gggtagggag tagggagtag
      361 ggagtaggga gtagggagta gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnntaggg ggagtggatc ggcggctcag ccgctcccgt gcgaaacgat ttgccgcccg
      481 gcctgcacgc cagcagtcct gctactccct actccctggt cctagtcgaa cagcttcgac
      541 accgactcct cgttggcgat gcggcggatg gcctcgccca tcagcggcgc gcaggagacg
      601 gcgcggatct tcttgcaggc cttgacgggg tcggacagtt caatggagtc ggtgaccacc
      661 agttccttga gcaccgaggc ggccacgcgg tcggctgcgg cccccgacag cacgccgtgg
      721 gtgacgtagg cgctgacctc ggtcgccccg gcgtcgctga gcgccttggc cgcgttgcac
      781 agggtgccag cggaatcgac gatgtcgtcg aacaggatgc agcggcggcc ggcgacctcg
      841 ccgatgatgt tcatcacctc gctctcgccc ggccccgagc gccgcttgtc gacgatcgcc
      901 aggtccgcgt cgatgcgctt ggccagggcc cgggcccgga ccacgccgcc gacgtcgggc
      961 gagacgatca gcaggtcctg ggtcttgccg tagtggctgc ggatgtcctg ggccagcagc
     1021 ggcacgggca gcaggttgtc ggtcgggatg tcgaagaagc cctggatctg gccggcgtgc
     1081 agatccatcg tcagcacccg gtcggctccg gcgcgggtga tcaggttggc caccagcttg
     1141 gccgagatcg gcgtgcggcc gccggtcttg cggtcctgcc gggcgtagcc gaagtagggc
     1201 atcaccgccg tgatccgccg tcccgaggcc cgcttgaggg cgtcgatgca gatcagcagc
     1261 tccatcaggt tgtcgttggc cgggaagctg gtcgactgga tcacgaacac gtcctcgccg
     1321 cggacgttct cgtcgatcgt cacgaacacc tcgttgtcgg cgaaccgccg cacctgggcg
     1381 cgggtcagcg ggatgtcgag gtattcggcg atatgggtcg cgagcgtgcg gttcgagttg
     1441 ccggctagca gcttcatgtc gacgactctt cgcgctgggg acgttgggcg gcttctagca
     1501 ggggaggcga agggggcaag gcggattcca tcccggcgaa ggccgggacc caggtgtttt
     1561 tgaagcagcg gctcaccttt ttcggtttgc caactggctt gagccgtgag ccgaagctct
     1621 gaaaaaacct gggtcccggc cttcgccggg atgagcggaa ggggtggttg gggctctcat
     1681 tgagagaggg aaatccattc aactcatgca ggccagcgtg ccggacgtgc cgggttggtc
     1741 aagggtggcc tccggccatc gcgccgagac gcgaagcgcc cttgagcaac ccggaacgcc
     1801 cggcgatgat ctcgccatga gatgaagggc ggggtgtggg cgcttgcagc cctgacaatc
     1861 gggcggtgcg tggcccgatc agaaagggag cgacttccat gttggtggga ctgatcggtg
     1921 cattggcgct agcatcgaca tccgctccac cggtcgatct cgacatcgcg cccggattca
     1981 gcggagtgcc ctgtgtcccc acgagaatta tcagggttgg ggcggatgga tcgctggccc
     2041 ccaagggaca atccatccag aagtcgcgtg ttgtgccggc cttaggtccg gtgagcggcg
     2101 gccaatgcga gcgcgtcaac atgacggttg atgacggagc cccatacgga gccgtgttcg
     2161 acctgatctc aattctgcgg gccgccgggt tccgtcagat cgcgctggtt gaccggcctt
     2221 gggaagacgc cgccccctaa gccaccttca cccgcgccgc gctctccggc agatcctcgc
     2281 cgaacgcgcg ctggtagaac tccgccacgg tcgggcgttc gagttcgtcg cacttgttca
     2341 ggaaggtcat ccggaacgcc agggccaggt cgccgccgaa gatctcggcg ttctgggccc
     2401 aggtgatcac cgtgcgcggg ctcatgaccg tggagatgtc gccgttcatg aaggcgttgc
     2461 gggtcatgtc ggcgacgcgg accatggcgg ccagggtgcg gcggccttcc ggggtggcgt
     2521 atttggggtt cttggccagc acgatctcgg cctcggcgtc gtgctcgagg tagttgaggg
     2581 tcgtgacgat cgaccagcgg tccatctggc cctgattgat ctgctgggtg ccgtgataga
     2641 ggccggtggt gtcgcccagg ccgatggtgt tggtcgtcga gaacagccgg aaatacgggt
     2701 tggcgcggat cacgcggttc tggtcgagca gcgtcagctt gccgccggct tccagcaccc
     2761 gctggatcac gaacatcacg tccggacggc cggcgtcgta ctcgtcgaac accagcgcca
     2821 tcgggcgctg caggcaccag ggcaggatgc cttcgcggaa ttcggtgatc tgcttgccgt
     2881 ccttcaggac gatggcgtcc ttgccgacca ggtcgatgcg gctgacgtgg ctgtcgaggt
     2941 tgacccgcac catcggccag ttgagccggg cgcagacctg ctcgatgtgc gtcgacttgc
     3001 ccgtgccgtg gtagccctgg atcatcaccc gccggtcgaa ggccaggccc gcgacgatcg
     3061 ccagggtggt ctgcgggtcg aagcggtagg cctcgtcgag atccggcaca tgggggtcgc
     3121 gctggctgaa cgccggcacc atcatgtcgg agtcgacgcc gaacacgtcg cggatggaaa
     3181 cctgcttgtc cggaacgaga ctcagcaacg ggtcgggggt ggtgtcggtt tggtcagcca
     3241 tgatggtttg accgattagc gccttatcag cgttcagcaa acattcgttc gaggaaaaaa
     3301 cggtgcagac ggcgtgatcg gcgggcgacg gccgggcgcg tggccggccc gggcccgcca
     3361 ggagccgggg tgcgcgggtt caggccagcc cggccttctt cagcgtcttc caggccttca
     3421 gcacgcgttg cagcttgtgc tcggcgctgc ggtcaccacc gttggagtcg ggatggcact
     3481 tcttgaccag ctcggtgtag cgcgtgcgga tcagggcctt ctcggctgtg tcgggcaggt
     3541 cgaggtcagc cagggcgttg cgttccagct tgcccagggt gcgaccatcg gcccgggtgg
     3601 ccggcgcgtc gacgcccgaa cggtcggccc ggccgaagcc gaacagactg aacgggtcgt
     3661 tccagcccat cggccccttg gcctgggcgg ccgcctcgcg cgagcggttg gaggccttga
     3721 agctccaggt cggacgctcg cccgtggcgc gctcggcctg ggcgcgggtg atcgcctcgt
     3781 cggtcatgcc ggcgaagaag ttccactgct tgttgtactc ggccgcgtgc gactggcaga
     3841 agtgatagaa ctgccccggc atgtcgcgcg acttcggcgc gcgggccgtg gccacgttca
     3901 ggcagtcgct gtggtcgcag cgccgctcgc ccggcttgag ggcgttgacg tcggcttccg
     3961 cctccgcttc gccgggcttg ggcgggcgga cacggatgtc gacgaatttg ggacggtatt
     4021 gaaaggcgtt cgccatgggc cgaagtatgg cccccaacct ttctcattca agaattgacc
     4081 ttcggaaaat gccctccata tcggactcca tccgcataaa actgaccgac gcgttctcgc
     4141 cgacacggct ggaggtggtt gacgactccg cccgccacgc cggacacgcc ggcgcgcggc
     4201 ccggcggcga gagccatttc aacgtcctga tagaggccca ggcgttcgcc ggcctcggca
     4261 aggtggcccg ccagcggcag gtctacggag tcctgtcgga gctgttggcc gggcccgtgc
     4321 atgccctgtc gctgaaggcg ctggcgcccg gggaaggctg acgcgccctc ggaaaattcg
     4381 gtgacaggga attcggtgac ctacagtccc agccccctca gggcccgctc caggtcggcc
     4441 tggcggaacg gcttctgcag catcggggcg ccacgatggg cctcgctgag gccgcccgag
     4501 ccgtagccgc tggcgaacac gaacggcacg ccgcgggcct tcagcgcgtc ggccaccgga
     4561 aagatcgggt cgccgccgag gttcaggtcg agcacggcgg cgtcgatctc cgcccccgac
     4621 gccagcggca tggcctcggc gatggtcggc gccggaccga ccacgcggca gccgaaatcg
     4681 gccagcatgt cctcaagcag catcgacacg agcatctcgt cctcgaccac gaggatccgc
     4741 cttccgtcca gcgccaagtc ggtcacttaa agcccctccg tcggcgaacc ggccgaaacc
     4801 ccacgcgccc ggcgcgtggg cctgtaacgc gcgggccggc ggaccgttcc cgcgccgtcg
     4861 gcccccgcgc ggtcagccgg agtcgatcca gccgccctcg ctgtccatcc gcgggctgat
     4921 gcgcagctcg gtgatctggt tgcgacgccg gcgggcgacc tcgaagcggt gatggaagaa
     4981 ggtgaaggtc tcgccctcct cgggaatggc ctgggcttcc tggatcaaca ggccggcgat
     5041 ggtcaccgcc tggtcgtccg gcaggtccca gtcgagcgcg cggttgagat cgcgcacggt
     5101 gacggtgccg gccgtctcga cccagccgtc gggctggacg cgcaggccct cgaccgtggc
     5161 gtcgtgctcg tcgtcgatct cgccgacgat ctcctcgagg atgtcctcca gcgacaccac
     5221 cccctgcagg ccgccgtact catcgaccac cagggcgaag tggttgcgct tcttgaggaa
     5281 ggcgttgagc tggtccttga gtttggtcgt ctcgggagtg aaccagggct tgcgcgacag
     5341 ggccatgacg tcgagggcgt cgaggttgac gtcggtctgg gccatgacct tgaccaggtc
     5401 gcgcacgtgc agcacgccga cgatgttgtc ggggtcgtcg cgccacagcg gcaggcggct
     5461 gtggttgcag tcgagggcct ggcgcaccag ttcgcgggcc ggcagggcgc agtcgagcat
     5521 ctcgaccgac ttgcggtgga ccatgacctc cgagacgtcc atctccgaca ggtcgagcac
     5581 gccgccgagc atgcgccggt cacggctttc gaccacgccc tcggagtggt ggtactcgac
     5641 cgcgccgcgg atctcctcgt gggccgccag cacgtcggtc tccatcgaca ggttgatgcc
     5701 gaacggccgc agggtctggc gcacgatcca ttgcgccgcc cgggccagcg ggccgaaaat
     5761 cctgaccagc cagaaggtcg gcagcgacag gaaacgggcg accacctcgg gccgggtgat
     5821 ggccagggtc ttgggcagga tctcgccgaa caccaccacc agcacggtca tgatcacggt
     5881 cgaggccagc acccctatgg ccccgggaaa ggccgccgcc agcacgctgg tggtcagggc
     5941 cgaggcgccg atgttgacca cgttgttgct gagcaggatg gccccgatca tgttctcctg
     6001 atcgcccatc atgccgttga cgcgggccgc ggcccggtcg ccgtcgcgct cgagctggtg
     6061 catgcggccg cggctggcgg cggtcattga ggtttcggcc gccgagatca gcgcgccgat
     6121 gcccagcagg atcagcaggg atggcgcgag gcccagcagc aaggcccaga tgctcacgac
     6181 gtggctcctt gttcgatcag gaagcggcgc agggtgtcgg gatcgacgcc cttcgccgtg
     6241 aaggcctcgc cgatccggcg ggcgaggatg aaggtcaggg cgccgccctg ggccttcttg
     6301 tcttggccca tgtgatggat caggcggtcg gcgtcgaacg gctggcccgg gacgtcggcc
     6361 atgccggcgg gcaggccggc ggtggccagg gcgcgctcga cccgcgccgc ctcggccgga
     6421 tcgcactggc cgagaaaggc cgagaagcgg aaggccaggc cgcagccggc cccgaccgcc
     6481 tcgccgtgct tgagggtctc gccgtagccg gtctcggcct ccagggcgtg gccgaaggtg
     6541 tggccgaggt tgagcagggc gcggcgaccc tgttcggtct cgtcctcggc gacgatctcc
     6601 gccttcattt cgaccgacag gccgacggcg tgggccaggg cgtcggtgtc gcggtcgagc
     6661 acggcccggc cgtgctgttc aagccaagcg aagaagccgg cgtcgccgag caggccgtac
     6721 ttgatcacct cggcgtagcc gcaggccatt tcgcgggccg gcagggtgca gagcacgtcc
     6781 aggtcggcca gcaccagccg gggctgatgg aaggcgccga tcaggttctt gccgcgcggg
     6841 gtgtcgatgg cggtcttgcc gccgaccgag ctgtcgacct gggccagcag gctggtcggg
     6901 acctggatga agtcgatgcc gcgcttgtag atggccgccg cgaagccggc caggtcgccg
     6961 accaccccgc cgccgaaggc gacgatcagg tcgccgcggt cgagccgcag ctcgagcagc
     7021 cggtcgctga gctcggccag gcccgcgaag ctcttgctct cctcaccggc cggcacggtg
     7081 atcatgtcga ccgccacccc ggcccgttcc agcgagaccg acagccgctc gccatggtgc
     7141 tcgccgacgt tgctgtcggt gacgatggcc gtgcgccggg tcttcagcag cggggcgatg
     7201 tgctcgccgg cgcggtcgat caggcccggg ccgaccagca cctcgtagga ccggccgccg
     7261 agggccacgg ggatgctgcg gatcacggct cggctccggc aaggtgggcg gtcagggcgc
     7321 gcaggacggc ttcgaccgct tcatggtggg gcgtgtcgcc ggtctcgact gtgatgtcgg
     7381 cctgggcgta gatcgggtcg cgcacgaccg acagggcggt cagcacctcc agcgggtcct
     7441 tgccgcggac cagcgggcgg gtgtccttgc ggccgatgcg gcgggccagc agggccaggt
     7501 cggccttgag ccagaccgag acggcccgct gcttgatcag ggcccgggtc tcgtcgttga
     7561 tgaaggcccc gcccccggtg gccagcacat gcgggccctc gtccatcagc cgggcgatca
     7621 cccgacgctc gccgtcgcgg aaggcggcct cgccgaaggt ctcgaaaatc tcggagatgg
     7681 tccgtccggc ggcggcctcg acctcggcgt cggcgtcacg gaacggcagg tcgagcacgg
     7741 cggccagccg acggccgaca ctgctcttgc ccacgcccat gagtccgacg agggcgatgg
     7801 tcttgcggcg aaggcggggg tcggcggtca tggccgtgct tggcttacac gagtgggggc
     7861 ggtcgagcca agcattgaac ccctttcacg gccggggaag cgggatgagc gaaggaggcc
     7921 gggcgcgggc gcgacgccgt ccgtcggccg atcgcatctc cctcaccgca agacggaggt
     7981 gttaaggtgg gccatgcgcc cgcttgttct cgccgccgcc cttgccctcc tcgccgcgcc
     8041 cgccggcgcc caggtcgagg tcacgcaact tgcggcgccc gactatttct cgctcggtga
     8101 ccgtgacagc ggcctgccgg cggacctgtg gcgcgggacg tcgccggccg tggcgcgcga
     8161 cgtcattccg cagatcggcg tcaagctgct gagccccgcc gccgcgcgtc tggcccgcca
     8221 cctgctcg
//
LOCUS       DAORNX010000076         8186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6465_length_8186_cov_7.235026, whole genome shotgun sequence.
ACCESSION   DAORNX010000076 DAORNX010000000
VERSION     DAORNX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6465_length_8186_cov_7.235026"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2819)
                     /gene="metH"
                     /locus_tag="PLO65_04975"
     CDS             complement(150..2819)
                     /gene="metH"
                     /locus_tag="PLO65_04975"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRD27635.1"
                     /translation="MRPVFVNIGERTNVTGSAKFRKLVAEGDYAAALSVARQQVEAGA
                     QIIDVNMDEGLLDSKAAMVTFLNLIAAEPDIARVPVMIDSSKWEVIEAGLRCVQGKAI
                     VNSISMKAGEAEFIAQARLCLRYGAAVVVMAFDEEGQADTADRKVAICARAYRILVDE
                     VGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEATRRIKQLLPYARVSGGVSNVSFSF
                     RGNEPVRRAIHSVFLYHAINAGMDMGIVNAGDLPVYDDIEPELREAVEDVILNRTPRA
                     GGSQTERLVDLAPKYKGDGSKVQVADQAWRGLPVAERLAHALVNGITEFIDADTEEAR
                     LAAERPLHVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAWLMPFMEAEKE
                     GKPREQAGRVLMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPADRILDEARAHG
                     VDIIGLSGLITPSLDEMVFVASEMERRGFDIPLLIGGATTSKTHTAVKIAPAYRAAST
                     TYVLDASRAVGVVSQLLSKTDKAAFEAQTREDYVKVREQFARGQEQKTRTPIARARAN
                     RFAIDLAASPPPKPSFTGTRAFDSYDLKTLAKYIDWTPFFASWELVGRFPDILTDDVV
                     GEAASDLWKDAQAMLARIVEENWFEARGVVGFWPAQADGDDIVVYADESRTTELTRLH
                     TLRQQMAKSGDKANLALSDFIAPIGGPADWIGGFAVTAGHGEPEIAKRFKEAGDDYSA
                     ILAAALADRLAEAFAEAMHHRVRTELWGYAPDEAFDLDHLIGERYRGIRPAPGYPAQP
                     DHTEKATLFKLLDAEAQTGIKLTESFAMSPAAAVSGLYFSHPDAHYFGVGRIDRDQVV
                     DYAARKGWPLATAERWLAPILAYDPQAERDAAA"
     gene            complement(2819..3379)
                     /locus_tag="PLO65_04980"
     CDS             complement(2819..3379)
                     /locus_tag="PLO65_04980"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD27636.1"
                     /translation="MASTYDALDDRLIAFIRAQKLFFVATAPLSAQGAVNVSPKGYDS
                     LAVIDPRTVAYVDLGGSGIETLAHVRENGRLTLMFCAFEGPASILRLYGRGEAVNFDE
                     PGFADRMALFPAFGRARSVITLHIERIADSCGWGVPFMDFRGERDQLRRHVDHRPEPE
                     WRERRYAGNATSIDGLPGLVRKSLVP"
     gene            complement(3380..3748)
                     /locus_tag="PLO65_04985"
     CDS             complement(3380..3748)
                     /locus_tag="PLO65_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27637.1"
                     /translation="MALALSPIEAEQIRDLRARLNRVPAGSLGDKSVGPLSKGFFVVC
                     GRVGLAPQTGGEGFASVAELKTAIAGADLIRLKQIVGRLQGLQAQDFKIWRTLFNHGV
                     LQAVLTRRMILGGREPPAEV"
     gene            complement(3750..4826)
                     /locus_tag="PLO65_04990"
     CDS             complement(3750..4826)
                     /locus_tag="PLO65_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRD27638.1"
                     /translation="MTRPDTRQDRIAALKAAAKQRILVLDGAWGVMIQRRGLSEADFR
                     GDRFADHNGQLKGNNDVLCLTRPDIIAELHDAYFAAGADISESNTFSATWIAQRDYGL
                     ESAAYDINLEGAKLARAAADRWTEKTPDKPRFAAGSIGPLNVMLSMSSDVNDPGARTV
                     TFDQVYEAYREQVRGLYDGGVDLFLIETITDTLNCKAAIKAILDLRDEDGHEALPIWI
                     SGTITDRSGRTLSGQTVEAFWNSVRHAKPFAVGLNCALGADLMRPHIAELSRIADTLV
                     AAYPNAGLPNAMGQYDEEPHQTACSLHEWAGSGIVNIVGGCCGTTPDHIAHVARSVAG
                     MAPRAVPDRPTAMRLAGLEAFELA"
     gene            complement(4823..5686)
                     /gene="bla"
                     /locus_tag="PLO65_04995"
     CDS             complement(4823..5686)
                     /gene="bla"
                     /locus_tag="PLO65_04995"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HRD27639.1"
                     /translation="MRRRIWIPAALAAVALAGVAIAQSMPPEWTRPTAPVKVGDNLYY
                     VGSEGLSAWLIATPKGLILIDGPMEQNVAGVEANIRALGFKLSDVEILLNTHAHFDHA
                     AGLAQLKRDTGAALLASAGDKPLLEKGVYPGSEDVAWLRFKPVKVDAVLKDRDTVSLG
                     GVTLTANITPGHTPGCTTWTFPVKIDGVVRKALLWCSTTVAGNRLAPRPQYPRIVADY
                     RASFVRLAAMKADVFLAPHPEQFGFAAKRAAMAPGKPSPFVDPAELGRRVAESRNAFE
                     AELARQQGAAR"
     gene            complement(5683..6609)
                     /gene="metF"
                     /locus_tag="PLO65_05000"
     CDS             complement(5683..6609)
                     /gene="metF"
                     /locus_tag="PLO65_05000"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRD27640.1"
                     /translation="MTPPSALGPVARAGGRDSGVRVSFEFSPPKTPQAEESLWAAIRR
                     LEPLNPSFVSVTYGAGGSTRERTHRTVKRMLDETTLKPAAHLTCVGASREEVDAVVRD
                     YWESGIRHIVALRGDPPGAIGGAYVPREDGYLNATELTRGILAVAPFEVSVGLYPQVH
                     PESAGIDHDIDVLKAKVDAGATRAITQFFFDTDVFLRFMDKVRKAGVTIPISPGIMPV
                     SNFKGLQRMAEPCGIPLPGWLGGLFDGLDDDPETRRLIAGSVAAEMCARLAEEGFSDF
                     HFYTLNRADLVYAICRVLGVREAPIPPKEAAA"
     gene            complement(6606..7577)
                     /locus_tag="PLO65_05005"
     CDS             complement(6606..7577)
                     /locus_tag="PLO65_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD27641.1"
                     /translation="MALSSGEAVEILRAAGEPTRLRILALLSREELSVLELCTVLGQS
                     QPRVSRHLKLLAEAGLVERFPDGAWVFYRLASGGGGRALADRALDLIDPADAALSRDA
                     DQLSRVAAERGSEAAAYFARNAARWDEIRSLHVAEAEVEAAIQAAVGDEAVGLLVDLG
                     AGTGRMLTLLGGRARRAVGLDLSQQMLNIARVNVGEAGLPACELRHGDIFDTGLPDAG
                     ADLVTVHQVLHYLGDPAAAVAEAARLVAARGRLLIVDFAPHGLEFLRELHQHRRLGFS
                     DEEMVRWLEAAGLTLESAAALPPATTAGVTVKIWTARRPTLAERNAA"
     gene            7753..>8186
                     /locus_tag="PLO65_05010"
     CDS             7753..>8186
                     /locus_tag="PLO65_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27642.1"
                     /translation="MKTFARRRVLVVDDNAHARTFLAQILGAVDVEVLQADGAAQALR
                     ALATQPVDAVILDMFMAPMDGIALTREIRASDSGQVAALPVVMASAQASREVVRGAVA
                     AGVSGFIAKPFTPAAVLRQLAVALAAPARRAVPAPSGRPTPVR"
BASE COUNT         1222 a         2831 c         2870 g         1263 t
ORIGIN      
        1 cgcccgccga ggagtcggcc gcccctcccc ccgcgacggc gatcgacgcg ctgctgccgg
       61 actccggccc cgcgagcgaa acgcccgcgc aggaagctcc gtccgaggac ccggctcctc
      121 cagccccgct gaaggggccg cagtaacggt caggccgccg cgtcgcgctc cgcctgaggg
      181 tcgtaggcga ggatcggcgc cagccagcgt tccgcggtcg ccagcggcca gcccttgcgg
      241 gcggcgtagt cgacgacctg gtcgcgatcg atccggccga cgccgaaata gtgggcgtca
      301 gggtggctga agtagagccc gctgaccgcc gcggccgggc tcatggcgaa gctctccgtc
      361 agcttgatgc cagtctgagc ctccgcatcc aacagcttga acagggtggc cttttcggtg
      421 tgatccggct gggccggata gcccggcgcc gggcggatgc cgcgatagcg ctcgccgatg
      481 aggtggtcga ggtcgaaggc ctcgtccggg gcatagcccc acagctcggt gcgcacgcgg
      541 tggtgcatgg cctcggcgaa ggcctccgcc aggcggtcgg ccagggccgc cgcgaggatg
      601 gccgaatagt cgtccccggc ctccttgaac cgtttggcga tctccggctc gccatgaccg
      661 gccgtgaccg cgaaaccgcc gatccagtcg gccggcccgc caatcggcgc gatgaagtcg
      721 gacagggcca ggttggcctt gtcgccgctc ttggccatct gctggcgcag ggtatgcagc
      781 cgggtcagct cggtggtccg gctctcgtcg gcatagacca cgatgtcgtc gccgtcggcc
      841 tgggccggcc agaagccgac cacgccgcgg gcctcgaacc agttctcctc gacgatcctg
      901 gccagcatcg cctgggcgtc cttccacagg tcgctggcgg cctcgccgac cacgtcgtcg
      961 gtcaggatgt ccgggaaccg cccgaccagc tcccagctgg cgaagaacgg cgtccagtcg
     1021 atgtatttcg ccagtgtttt cagatcgtag gagtcaaagg ctcgcgtgcc ggtgaagctc
     1081 ggcttcggcg gcggcgaggc ggccaggtcg atggcgaagc ggttggcccg cgcgcgcgcg
     1141 atcggcgtgc gggtcttctg ctcctggccg cgcgcgaact gctcgcggac cttcacatag
     1201 tcctcgcggg tctgggcctc gaaggcggcc ttgtcggtct tcgacagcag ctgcgagacg
     1261 acgcccacgg cccgactggc gtcgagcaca taggtggtcg aggcggcgcg gtaggccggg
     1321 gcgatcttca ccgccgtgtg ggttttgctg gtggtcgccc cgccgatcag cagcgggatg
     1381 tcaaacccgc gccgctccat ctccgaggcg acgaagacca tctcgtccag cgacggagtg
     1441 atcaggccgg acaggccgat gatgtcgacg ccatgcgccc tggcctcgtc caggatgcgg
     1501 tcggccggga ccatcacgcc caggtcgatg acctcgtagt tgttgcactg caggaccacg
     1561 ccgacgatgt tcttgccgat gtcgtggaca tcgcccttga cggtcgccat cagcacccgg
     1621 ccggcctgtt cgcgcggctt cccctccttc tcggcttcca tgaagggcat cagccaggcg
     1681 accgcctgtt tcatcacccg ggccgacttg accacctggg gcaggaacat cttcccggcc
     1741 ccgaacaggt cgccgacgat gttcatcccg tccatcagcg ggccttcgat gacatgcagg
     1801 ggccgctcgg cggccagccg cgcctcctcg gtgtcggcgt cgatgaactc ggtgatgccg
     1861 ttgaccagcg cgtgggccag ccgctcggcg accggcaggc cacgccaggc ctggtcggcg
     1921 acctggacct tcgatccgtc gcccttgtac ttcggagcga gatccacgag ccgctcggtc
     1981 tggctcccgc cggccctcgg cgtgcggttg aggatcacgt cctcgacggc ctcgcgcagt
     2041 tccggctcga tgtcgtcgta gaccggcagg tcgccggcgt tgacgatgcc catgtccatg
     2101 ccggcgttga tcgcgtggta caggaacacg ctgtggatcg cccgacgcac cggttcgttg
     2161 ccgcggaagc tgaacgagac gttcgacacc ccgcccgaca cccgcgcata gggcagcagt
     2221 tgcttgatcc gccgcgtcgc ctcgatgaag tcgacggcgt agttgtcgtg ctcctcgatg
     2281 ccggtcgcca cggcgaagat gttcggatcg aagatgatgt cctcgggcgg gaagccgacc
     2341 tcgtcgacga ggatgcgata ggcgcgcgcg cagatggcca ccttgcggtc ggcggtgtcg
     2401 gcctggccct cctcgtcgaa agccatgacc acgacggccg ccccgtagcg caggcacagc
     2461 ctggcctggg cgatgaactc ggcctcgccg gccttcatcg agatggagtt gacgatcgcc
     2521 ttgccttgca cacagcgcag gccggcctcg atgacctccc atttggagct gtcgatcatc
     2581 accggcaccc gggcgatgtc cggttcggcg gcgatcaggt tgaggaaggt gaccatggcc
     2641 gccttcgaat ccagcaaccc ctcgtccatg ttgacgtcga tgatctgcgc gccggcctcg
     2701 acctgctggc gggcgacgga cagggcggcg gcatagtcgc cctcggccac cagcttgcgg
     2761 aatttggccg agccggtgac gttggtgcgc tcaccgatgt tgacgaagac aggacgcatc
     2821 aggggaccag ggactttcgg acaaggccgg gcaggccgtc gatggaggtg gcgttgccgg
     2881 cgtagcgccg ctcgcgccat tcgggttcgg ggcggtgatc gacatggcgg cgaagctggt
     2941 cgcgctcgcc gcggaagtcc atgaaaggga cgccccagcc gcagctgtcg gcgatgcgct
     3001 cgatatgaag ggtgatcacc gaacgggcgc gaccgaaggc cgggaagagg gccatcctgt
     3061 cagcgaaccc cggctcgtcg aagttcaccg cctcgccgcg gccgtagagg cgcaggatac
     3121 tggccggccc ctcgaaggcg cagaacatca gggtgaggcg gccgttctcg cggacatggg
     3181 ccagggtctc gatccccgag ccgcccaggt cgacataggc caccgtgcgg ggatcgatca
     3241 ccgcaaggct gtcgtagccc ttgggcgaga cgttcaccgc gccctgcgcg gaaagcggcg
     3301 cggtggccac gaagaacagc ttctgggccc tgatgaaggc gatcaggcga tcgtcgagcg
     3361 cgtcataggt gctggccatt cagacctccg ccggcggttc gcgaccgccg aggatcatgc
     3421 ggcgggtcag cacggcctga aggaccccgt gattgaacag ggtgcgccag atcttgaagt
     3481 cctgggcctg cagaccctgc agccggccga caatctgctt gaggcggatc aggtcggccc
     3541 cggcgatggc ggtcttcagt tcggccaccg aggcgaagcc ctcgccgccg gtctgcggcg
     3601 ccagtccgac ccggccgcag acgacgaaga agcccttgct caaagggccg acggacttgt
     3661 cgcccagcga cccggccggg acgcggttga gccgtgcgcg gaggtcgcgg atctgttccg
     3721 cttcgatggg ggaaagtgcg agcgccatgt cacgccagct cgaacgcttc cagcccggcc
     3781 agccgcatgg cggtcggacg atccggcacg gcgcgcgggg ccatgccggc caccgaccgg
     3841 gcgacatggg cgatgtgatc gggcgtggtg ccgcagcagc cgccgacgat attgacgatg
     3901 ccgctcccgg cccattcgtg cagcgaacag gcggtctggt gcggctcctc gtcgtactga
     3961 cccatggcgt tgggcaggcc ggcgttgggg taggcggcga ccagggtgtc ggcgatgcgc
     4021 gacagctcgg cgatgtgcgg ccgcatcagg tcggccccga gcgcgcagtt gaggccgacg
     4081 gcgaacggct tcgcatggcg cacgctgttc cagaaggcct cgaccgtctg gcccgacagc
     4141 gtgcggcccg agcggtcggt gatcgtgccg ctgatccaga tcggcagcgc ctcgtgaccg
     4201 tcctcgtcgc gcaggtcgag gatcgccttg atggccgcct tgcagttcag ggtgtcggtg
     4261 atggtctcga tcaggaacag gtcgaccccg ccgtcgtaga ggcccctcac ctgctcgcga
     4321 taggcttcgt agacctggtc gaaggtcacc gtccgcgcgc cggggtcgtt cacgtccgag
     4381 gacatcgaca gcatgacgtt cagcggcccg atcgagccgg ccgcgaagcg cggcttgtcc
     4441 ggcgtcttct cggtccagcg atcggcggcg gcccgggcca gcttcgcccc ctcgaggttg
     4501 atgtcgtagg ccgcgctttc gaggccatag tcgcgctggg cgatccaggt cgcgctgaag
     4561 gtgttggatt cgctgatgtc ggccccggcg gcgaaatagg cgtcgtgcag ttcggcgatg
     4621 atgtcgggcc gcgtcaggca cagcacgtcg ttgttgccct tcagctgtcc attatggtcc
     4681 gcgaaccggt cgccgcggaa atccgcctcg gacaggccac gccgctggat catcacaccc
     4741 caggccccgt cgagcacgag gatgcgctgc ttcgcggcgg ccttcagggc ggcgatacgg
     4801 tcctggcgcg tgtcggggcg ggtcatcggg cggctccctg ctggcgagcc agctcggctt
     4861 cgaaggcatt tcgcgattcg gcgacgcggc ggccgagctc ggcggggtcg acgaacggac
     4921 tgggtttgcc cggcgccatg gccgcgcgct tggcggcgaa gccgaactgc tcggggtgcg
     4981 gcgcgaggaa gacatcggcc ttcatcgcgg ccagacggac gaagctggcg cggtagtcgg
     5041 ccacgatgcg cggatactgc ggcctggggg ccagccggtt gcccgccacc gtcgtgctgc
     5101 accagagcag ggccttgcgc acgacgccat cgatcttcac cggaaaggtc caggtcgtgc
     5161 agccgggcgt atggccgggt gtgatgttgg ccgtcagggt cacgccgccc agggaaaccg
     5221 tgtcgcgatc cttgagcacc gcgtcgacct tgaccggctt gaagcgaagc caggcgacat
     5281 cctcggagcc cggatagacg cccttctcga gcagcggctt gtctccggcg ctggccagca
     5341 gcgccgcgcc ggtgtcccgc ttcagctgcg cgaggcccgc ggcgtggtcg aagtgggcat
     5401 gggtgtttag caggatttcc acatcggaca gcttgaagcc gagcgccctg atgttggcct
     5461 cgacgccggc gacgttctgc tccatcggcc cgtcgatcag gatcagcccc ttcggcgtgg
     5521 cgatcagcca ggccgacagg ccctcgctgc cgacatagta gagattgtcg ccgaccttca
     5581 ccggcgcagt cggccgtgtc cactccggcg gcatcgactg ggcgatggcc acgccggcca
     5641 gcgcgacggc ggccagagcg gcgggaatcc agatccgccg tctcacgccg cggcctcctt
     5701 cggcgggatc ggggcctcgc gcaccccaag cacgcggcag atggcgtaga ccagatcggc
     5761 ccggttgagg gtgtagaagt ggaagtcgct gaagccttcc tcggccagcc gggcgcacat
     5821 ctcggcggcc accgaaccgg cgatcagccg acgggtctcg ggatcgtcgt ccagcccgtc
     5881 gaacaggccg cccagccagc ccggcagcgg gatgccgcag ggctcggcca ttcgctgcag
     5941 gcccttgaag ttactgaccg gcatgatccc cggcgaaatc gggatggtca ccccggcctt
     6001 gcggaccttg tccatgaacc gcaggaagac gtcggtgtcg aagaagaact gggtgatggc
     6061 ccgcgtcgcg cccgcgtcga ccttggcctt gagcacatcg atatcgtggt cgatgcccgc
     6121 gctctcggga tgcacctgcg gatagagtcc gaccgacacc tcgaacggcg ccacggccag
     6181 gatgccgcgc gtcagctccg tcgcgttgag atagccgtcc tcgcgcggaa cataggcccc
     6241 gccgatggcg cctggcgggt ccccgcgaag ggcgacgatg tggcggatgc cgctttccca
     6301 gtagtcgcgc accaccgcgt cgacctcctc gcgggaggcc ccgacgcagg tcaggtgggc
     6361 ggcgggcttc agcgtcgtct cgtcgagcat gcgcttgacc gtgcggtggg tccgttcgcg
     6421 ggtcgagccg ccggcgccgt aggtcaccga cacgaacgac gggttcagcg gctcgagccg
     6481 gcggatggcc gcccacaggc tttcctcggc ctgcggcgtc ttgggcggcg agaactcgaa
     6541 cgacacccgc acgccgctgt cgcggccgcc ggcccgggcc accggtccaa gagctgaggg
     6601 aggggtcatg cggcgttcct ttcggccagg gtgggccgac gggccgtcca gatcttcacg
     6661 gtcacgccgg cggtggtcgc cggcggcagg gcggcggcgg actccagggt cagcccggcg
     6721 gcttcgagcc agcggaccat ctcctcgtcc gaaaagccga gccgccggtg ttgatgcagt
     6781 tcgcgcagga actccagccc gtgcggcgcg aaatcgacga tcagcagccg cccgcgggcg
     6841 gccaccaggc gggcggcttc ggcgaccgcg gcggcgggat cgcccaggta gtgcagcacc
     6901 tggtggacgg tgaccagatc ggcgccggcg tcgggcaggc cggtgtcgaa gatatctccg
     6961 tggcgcaact cgcaggccgg caggcccgcc tcgccgacat tgacccgggc gatgttgagc
     7021 atctgctgcg acaggtccag gcccacggcc cgacgggcgc gcccgcccag cagggtgagc
     7081 atgcgaccgg tcccggcgcc cagatcgacc agcagcccga ccgcctcgtc gccgaccgcg
     7141 gcctggatgg ccgcctcgac ctcggcctcg gcgacgtgca acgagcggat ttcgtcccag
     7201 cgggccgcgt tgcgggcgaa ataggcggcc gcctccgacc ctcgctcggc ggccacccgc
     7261 gatagctggt ccgcgtcgcg cgacagggcc gcgtccgcgg ggtcgatcag gtcaagggcc
     7321 ctgtcggcga gggcgcggcc gcccccgcca gacgccaggc gatagaacac ccaggccccg
     7381 tcggggaacc gttcgaccag tcccgcctcg gccagcagct tcagatggcg tgagacccgc
     7441 ggctggctct gccccagcac agtgcacagt tcgagcaccg aaagttcctc gcgcgacaga
     7501 agggcgagga tgcgcagccg cgtcggctcg cccgctgcgc gcagtatctc gaccgcttct
     7561 cctgatgaca gcgccataag gatataaaga tatctttata tcctcaagtc aagcctcgcc
     7621 cggccgacgc agttcgcgcc gcccgggcca aacgtcaccc gatgcgggga ccgggcggcg
     7681 acgattcacc ggccagaaac catcgccgcc gccaagatgc ccggtatccc cacggaccaa
     7741 gaggcgcgca tcatgaagac cttcgcccgg cgccgcgtcc tggtcgtcga cgacaacgcc
     7801 catgcccgga cgttcctggc ccagatcctc ggagccgtcg atgtcgaggt cctgcaggcc
     7861 gacggcgcgg ctcaggccct gcgtgcgctg gcgacccagc ctgtcgatgc ggtcatcctc
     7921 gacatgttca tggcgccgat ggacggcatc gccctgaccc gcgagatccg cgcctcggac
     7981 tcgggtcagg tggccgcgct gccggtggtc atggcctcgg cccaggccag tcgcgaggtg
     8041 gtccgcgggg ccgtggcggc cggggtcagc ggcttcatcg ccaagccgtt cacccccgcc
     8101 gcggtgctgc gccaactggc cgtcgccctc gccgctccgg cccgccgggc ggtccccgcc
     8161 ccgagcggcc gaccgacacc cgtccg
//
LOCUS       DAORNX010000077         8123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6533_length_8123_cov_6.878285, whole genome shotgun sequence.
ACCESSION   DAORNX010000077 DAORNX010000000
VERSION     DAORNX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6533_length_8123_cov_6.878285"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..>1043
                     /gene="rnd"
                     /locus_tag="PLO65_05015"
     CDS             127..>1043
                     /gene="rnd"
                     /locus_tag="PLO65_05015"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRD27643.1"
                     /translation="MQPITTTAQLADFCKSLKGQPFIAVDTEFMRETTYWPKLCLIQV
                     AAPNGVEACIDPLADGLDLAPLLDILRDESVDKIFHAARQDVEIFNNLGAMPRPLFDT
                     QIAGMAAGFGEQIAYDALVRQMLKIELDKSSRFTDWARRPLSDNQLTYALADVTHLAE
                     LYPKLRARLERDGRLAWVEEEMAALTDPALYDVEPENAWKRLKPRKHTPKYLAVFKAV
                     AAWRERTAQQRDQPRGRILKDDAIDELAAQAPTDAAAMDRLRGVPKGFSGSKFGPDLL
                     EAIREALKDPDGYAPKIDRERGPQNAAAGA"
     assembly_gap    1044..1101
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1102..1306
                     /locus_tag="PLO65_05020"
     CDS             <1102..1306
                     /locus_tag="PLO65_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRD27644.1"
                     /translation="SKLLATVSDLEKIANDDNADVGALTGWRREAFGADALKLKRGEL
                     ALVLDGARVRVVEVRRAPKAAAG"
     gene            complement(1487..2050)
                     /locus_tag="PLO65_05025"
     CDS             complement(1487..2050)
                     /locus_tag="PLO65_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27645.1"
                     /translation="MTIFEYVMILLSVVISLGLARLLETHAQLLKRGDKVRWSPTYLA
                     WLLILLLSHIDLWASLWQVHEHARWNAADIGATLAAAALLFYASIFATPDINNEVRTD
                     LWTFHLANRRRYLGALLGYMIVGAWLNVTLMRETFSATNATAAAPAIVLMLVAIFIAN
                     RWIQRLMVALTLVLITLYFAQYLPAIG"
     gene            2167..3012
                     /locus_tag="PLO65_05030"
     CDS             2167..3012
                     /locus_tag="PLO65_05030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD27646.1"
                     /translation="MSRPERLTALIYDFDGTLARGNMQETSFIPSLGMTPAQFWNETV
                     RPRTLAADGDDILMYMQLMLQTAREQGVTVTRRMLRDHGKDVPLFEGIRDGSWFDRIN
                     AFGAHYGLTVQHYILSSGLQEMIEGCPIHDRFEHVFASHYVFDEGDVAVWPAVGINYT
                     TKTQYLFRINKGVHNNWEHERINRYMPDEDRPVPFERMIFIGDGDTDVPTMKMMHTKG
                     GHSIAAYDPRTEPRDQDKLHRLIADDRVNFVAAADYREGTAMDLIVKGILGRIAVRER
                     TMPTG"
     gene            3087..3473
                     /locus_tag="PLO65_05035"
     CDS             3087..3473
                     /locus_tag="PLO65_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27647.1"
                     /translation="MRLNQVTASARDLAASIAFYEALGLRLIVRNDHYARFECPDADG
                     GEPPTFSLHLDPETAGVTRTVIYFEDDDLDATVNRLKAGGLAFDSDPADQSWLWREAR
                     LRDPAGNAVCLYRAGRIRRFPPWRLA"
     gene            complement(3470..3940)
                     /locus_tag="PLO65_05040"
     CDS             complement(3470..3940)
                     /locus_tag="PLO65_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27648.1"
                     /translation="MRALSLTVLLLLAPVAARAQSLDDSPPVYVASVYDRLAKGEAFE
                     APEALYTPRLQALWKDMERDAGGEVGRLDANYWTNSQDWTISGVTIGSAYADGHDDDR
                     MIVVARFRNMDRNEVIQFYFERLGGRWRLDDVVSLAGEPWTLSLVLKYGWAGGN"
     gene            4150..4506
                     /locus_tag="PLO65_05045"
     CDS             4150..4506
                     /locus_tag="PLO65_05045"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRD27649.1"
                     /translation="MLLTMMKAKLHRATVTQADLDYEGSIAIDRDLLDQSGILPHEQV
                     DVLNITNGARFTTYAIEAPRGSKVIGVNGAAARLVQKGDKVIVVTYCQLPAEEARNYN
                     PNVVLLDDANHVKHAA"
     gene            4565..5041
                     /locus_tag="PLO65_05050"
     CDS             4565..5041
                     /locus_tag="PLO65_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27650.1"
                     /translation="MRTPFIVLTLAGAGLGLAACEPTGPRGIDAELLNQQVGRAVGDP
                     NTCVLLVEKGSGKVVWRYGDHSTCASAMPSCQGAAMLSPDGLAQLAASGEVRTLSCDS
                     APDGASRVGWASGPVVPSAGAKYGDLAYAAAMEGPSVLPGREIKVRLEAAFRKAGM"
     gene            complement(5184..5921)
                     /locus_tag="PLO65_05055"
     CDS             complement(5184..5921)
                     /locus_tag="PLO65_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRD27651.1"
                     /translation="MKALFRAAALGALIVAGSAGGALAQTAAPSAAGAVFNATTLSLS
                     AGGEVSRRPDMATITLGVQTDAPTAAEALRLNAVRMNQVIAAIKRGGVADRDIQTSGL
                     NLSPQYVYQENLPPRLTSYQASNQVTIIVRDLTLLGQAVDASVNAGASNVGGVAFGLQ
                     NSDAAEDAARLEAVKALQAKAALYAQATGYRVARLVTLSEGGGYSPPAPMPIMAMARM
                     DKAESTPVEAGELKVRIDITATFELVR"
     gene            6040..6115
                     /locus_tag="PLO65_05060"
     tRNA            6040..6115
                     /locus_tag="PLO65_05060"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6073..6075,aa:Ile,seq:cat)
     gene            complement(6125..7069)
                     /locus_tag="PLO65_05065"
     CDS             complement(6125..7069)
                     /locus_tag="PLO65_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD27652.1"
                     /translation="MSDPTLSADAPLAVATGIIDAARADPARQAQVKAFFDEATFTVS
                     YVVRDPGSTACAIIDSVLDFDPASGRTASRSADAVIDHVRAEGLEVVWQLETHAHADH
                     LSAAPYLQEALGGRLAIGDRIQRVQETFGALFNAGPDFARDGRQFDHLFGDGERFAIG
                     GLQAVALHVPGHTPACMAYVIGDAVFVGDTLFMPDYGTARCDFPGGDAATLYRSIQRL
                     LSLPADTRVFLCHDYKAPGRDRYLWETTIGEQRRSNVHVHEGVTQEAFVALREARDRT
                     LDMPRLILPSVQVNMRAGHLPEPEDNGVRYLKIPLNAV"
     gene            7148..7921
                     /locus_tag="PLO65_05070"
     CDS             7148..7921
                     /locus_tag="PLO65_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD27653.1"
                     /translation="MTLITILSALMSGAVVGALLALFGGGGSVLATPLLLYVVGVRDP
                     HVAIGTSAAAVAVNALINLVGHGREGRVKWPCALVFAAAGLAGSFLGSTLAKQVDGQR
                     LLLFFAMAMAAIALSMLRRPRSEGDPAVRITWRFTLRLAPLGLLTGLAAGFFGIGGGF
                     LIVPGLMAATGMTLANAAASSLLSVALFGAATSANYALSGLVDWPIAGLFILGGAVGG
                     WVGLRGAGLLAGRALLGRRLFAGLVLIVAGYVAWRAVTG"
     gene            complement(7918..>8123)
                     /locus_tag="PLO65_05075"
     CDS             complement(7918..>8123)
                     /locus_tag="PLO65_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HRD27654.1"
                     /translation="SLARAYLRPAPWLLLDEPTEGLDSATEAVVVAALDRRLSRTGQG
                     VIIVSHRPLPLTLCAETIRAVDR"
BASE COUNT         1264 a         2750 c         2807 g         1244 t
ORIGIN      
        1 aggccgttcg gccgatcggt catggggtgc tcggggaggc ttcgcggcaa cgattgagaa
       61 cgaaaatgag gcgcaggggc ctgcatctct tgcagactcg gccccgggcg gcgctatagc
      121 tacccgatgc agccgattac gaccaccgcc cagctggcgg atttctgcaa gtccctgaaa
      181 ggtcagccgt tcatcgccgt ggacaccgag ttcatgcgcg aaacgaccta ctggcccaag
      241 ctctgcctga tccaggtggc ggcgcccaac ggcgtcgagg cctgtatcga tcccctggcc
      301 gacggacttg atctcgcccc cctgctcgac attctccgcg acgagagcgt cgacaagata
      361 ttccacgccg cccgccagga cgtggagatc ttcaacaacc tgggcgccat gcccaggccg
      421 ctgttcgaca cccagatcgc cggcatggcc gcgggcttcg gcgaacagat cgcctatgac
      481 gccctggtgc ggcagatgct gaagatcgag ctcgacaagt cgagccgctt caccgactgg
      541 gcccgccggc cgctcagcga caaccagctg acctacgccc tggccgacgt gacccacctg
      601 gccgagctct atccgaagct gcgcgcgcgg ctggagaggg acggacggct ggcctgggtc
      661 gaggaggaga tggcggccct gaccgacccg gcgctctatg acgtcgagcc ggagaacgcc
      721 tggaagcggc tcaagccgcg caagcacacg ccgaagtacc tggccgtgtt caaggcggtg
      781 gccgcctggc gtgagcgcac ggcgcagcaa cgcgaccagc cgcgcggccg gattctcaag
      841 gacgacgcga tcgacgagct ggccgcccag gcgccgaccg acgccgccgc catggaccgg
      901 ctgcgcgggg tgcccaaggg cttctcgggc agcaagttcg gccccgacct gctcgaggcc
      961 atccgcgagg cgctcaagga ccctgacggc tacgccccca agatcgaccg cgagcgcggc
     1021 ccgcagaacg ccgccgccgg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn ngtccaagct gctggccacg gtgtctgacc tggagaagat
     1141 cgccaatgac gacaacgccg acgtcggcgc cctgaccggc tggcggcgcg aggctttcgg
     1201 ggccgacgcc ctcaagctca agcgcggcga gctggccctg gtgctggacg gcgcgcgcgt
     1261 gcgcgtcgtc gaggtgcgcc gcgcgccgaa ggcggccgct ggctaggttc aatcgacatt
     1321 cagcggccga aggccgttcc tgcaccgatt gtccccgcga aggcggggac ccaagcttcg
     1381 tatgcgtccg atgcggcgcc tcaaagcctg cctgattgct tgggttcccg ccttcgcgag
     1441 aacaggcgga ctttgaaatg ccccgcagac tgaatgtcga atggtcctag ccgatcgcgg
     1501 gcaggtactg ggcgaagtag agggtgatca ggaccagcgt cagggccacc atcaggcgct
     1561 ggatccagcg gttggcgatg aagatcgcga ccagcataag cacgatcgcg ggcgccgccg
     1621 ccgtggcgtt ggtggccgag aacgtctcgc gcatcagggt gacgttcagc cacgccccga
     1681 cgatcatgta gcccagcagc gcgccgaggt atcgccgccg gttggcgagg tgaaacgtcc
     1741 acaggtccgt gcggacctca ttgttgatgt cgggcgtggc gaagatcgag gcgtagaaga
     1801 gcagagccgc ggccgccagc gtggcgccga tgtcggccgc gttccaccgg gcgtgctcat
     1861 gcacctgcca gaggctggcc cacaggtcga tgtggctcag cagcaggatc agaagccagg
     1921 cgagataggt cggcgaccat cgtaccttgt cgccgcgctt cagcagctgg gcatgggtct
     1981 ccagcaggcg cgccagaccc agcgagatga ccacggacaa caggatcatc acgtattcga
     2041 agatggtcat gcgctgtacc ccggggacgg gcgagaggct ggcacggcca tcgccggtcg
     2101 tgaaggggcg tttcccaacc ggcgcggccg gcgctatgtg tctcgtccaa cgagacagga
     2161 acgcaaatgt cccgacccga acgcctgacc gccctgatct acgacttcga cggcaccctg
     2221 gcccgcggca acatgcagga gaccagcttc attccgtcgc tggggatgac gccggcgcag
     2281 ttctggaacg agacggtccg gccgcgcacg ctggccgccg atggcgacga catcctgatg
     2341 tacatgcagc tgatgctgca gacggcgcgc gagcaggggg tcacggtgac ccgccggatg
     2401 ttgcgcgacc acggcaagga cgtgccgctg ttcgagggca tccgcgacgg cagctggttc
     2461 gaccgcatca acgccttcgg cgcgcactac ggcctgacgg tgcagcacta catcctgtcc
     2521 agcggcctgc aggagatgat cgagggctgc ccgatccatg accgcttcga gcatgtgttc
     2581 gcctcgcact atgtgttcga cgagggcgat gtggcggtct ggccggccgt gggcatcaac
     2641 tacacgacca agacccagta cctgttccgc atcaacaagg gcgtgcacaa caactgggag
     2701 catgagcgga tcaaccgcta catgcccgac gaggaccggc ccgtgccgtt cgagcggatg
     2761 atcttcatcg gcgacggcga caccgatgtg ccgaccatga agatgatgca caccaagggc
     2821 ggccactcga tcgccgccta cgacccgcgc accgagccgc gcgaccagga caagctccac
     2881 cgcctgatcg ccgacgacag ggtcaacttc gtcgcggccg ccgactaccg cgagggcacg
     2941 gccatggacc tgatcgtcaa gggcatcctg ggccgtatcg ccgtgcgtga acggaccatg
     3001 ccgacggggt agggggcgat ccaggcctgg cgggccaagt ggatatggat ctggcgccgt
     3061 gtcgccgggt ttcctaccta ccccccatgc gcctcaacca ggtcaccgcc tcggcccgcg
     3121 atctcgccgc ctcgatcgcc ttctatgaag ccctcggatt gcggctgatc gtccgcaatg
     3181 atcactacgc ccggttcgaa tgcccggacg ccgacggcgg cgagccgccg acgttttcgc
     3241 tacacctcga tccggaaacg gcgggcgtga cccggacggt catctacttc gaggacgacg
     3301 acctcgacgc caccgtgaac cggctcaagg ccgggggcct ggcctttgac agcgatccgg
     3361 ccgaccagag ctggctatgg cgcgaggcgc ggctgcgcga tcccgccggc aacgccgtct
     3421 gcctctaccg ggccggccgg atccgccggt tcccaccctg gcggctggcc tagtttccgc
     3481 cggcccagcc gtacttgagc accagcgaca gcgtccaggg ctccccggcg agcgacacca
     3541 cgtcgtcgag cctccaccgg ccgccgagtc tttcgaagta gaactggatc acctcgttgc
     3601 ggtccatgtt gcgaaaccgc gccaccacga tcatgcggtc gtcgtcgtgg ccatcggcat
     3661 aggcgctgcc gatggtgacg ccggagatcg tccagtcctg ggaattggtc cagtagttgg
     3721 cgtccagccg gccgacctcg ccgccggcgt cccgttccat gtccttccac agggcctgca
     3781 ggcgcggggt gtagagcgct tcgggcgcct cgaacgcctc gcccttcgcc agccggtcgt
     3841 agacgctggc cacatagacg ggcggggaat cgtccagcga ctgggcccgg gcggcgaccg
     3901 gcgccagcag caagaggaca gtgagcgaca gggcgcgcat ggcgactccg ggaaggccga
     3961 gacggcgagg ctgcgccgcg cgcgcgccgg tgtcaaagcg ccgcccgccc gcgcgtccgc
     4021 cgaaagtgat cagcgttcat gatgcagcgc aattgaaccg gcggacctta gtcgttatat
     4081 gccaagcctc ccaacgggcc gtgtggattg tgcggtgccg cgattgtccc gagtagaagg
     4141 gaagacgcca tgctgctgac catgatgaag gccaagctgc accgcgccac ggtgacccag
     4201 gccgacctcg actacgaggg ctcgatcgcc atcgaccgcg atctgctcga ccagtccggc
     4261 atcctgccgc acgagcaggt cgatgtgctc aacatcacca acggcgcgcg cttcaccacc
     4321 tacgccatcg aggccccgcg cggcagcaag gtgatcgggg tcaatggcgc ggccgcacgc
     4381 ctcgtgcaga agggcgacaa ggtcatcgtc gtgacctact gccagctgcc ggccgaagag
     4441 gcccgcaact acaatccgaa cgtcgtgctg ctggacgacg ccaaccacgt caagcacgcc
     4501 gcctgatcca gcgcattgag tgacggcgcg ggatggcgca gggtctccgg cacaggagga
     4561 tcccatgcgc acgcctttca tcgtcctcac cctcgcgggg gccggtctcg gtctcgccgc
     4621 ctgtgaaccg accgggcccc ggggcatcga cgccgaactg ctgaaccagc aggtcggccg
     4681 cgccgtgggc gatcccaaca cctgcgtgct gctggtcgag aagggcagtg gcaaggtggt
     4741 ctggcgctat ggcgaccatt cgacctgcgc cagcgccatg ccatcctgcc agggcgcggc
     4801 gatgctcagt cccgacggcc tggcccagtt ggccgcgtcg ggggaggtgc ggaccctgag
     4861 ctgcgacagc gccccggacg gcgccagccg ggtcggctgg gcctccggtc cggtggttcc
     4921 ctcggccggc gcgaaatacg gcgacctggc ttacgccgcg gccatggaag gccccagcgt
     4981 cttgccgggc cgggaaatca aggtccggct cgaggcggcg ttccgcaagg cgggtatgtg
     5041 agggcagggc ctgggcgtag cgaataggga gtagcaaggg gcgggcttga agcggcggct
     5101 ggcgaagcgc ccgtgtcgcg gccgcctcgc cggtctgctc cctgctccct acgcccgtcg
     5161 ccgcgctccc gggcccccgc cgcctaccgc accagctcga acgtcgcggt gatgtcgatc
     5221 cgcaccttca gctcaccagc ctcgacgggc gtggactcgg ccttgtccat ccgggccatg
     5281 gccatgatcg gcatcggcgc gggcgggctg tagccgccac cttcgctgag ggtgaccagc
     5341 cgcgccacgc gatagcctgt ggcctgggcg tagagcgcgg ccttggcctg cagcgccttg
     5401 acggcctcga gccgggccgc gtcctccgcc gcgtcgctgt tctgcaggcc gaaggcgacg
     5461 ccgccgacat tgctggcgcc ggcgttgacc gaggcgtcga ccgcctggcc cagaagggtc
     5521 aggtcgcgca cgatgatggt cacctggttg gaggcctggt agctggtcag ccgcggcggc
     5581 aggttctcct ggtagacgta ctgcgggctc aggttgagcc cgctggtctg gatgtcgcgg
     5641 tcggcgacgc cgccgcgctt gatcgccgcg atcacctggt tcatccgcac ggcgttcagc
     5701 cgcagggcct cggcggcggt cggggcgtcg gtctgcacgc ccagggtgat ggtggccatg
     5761 tcgggcctgc ggctgacttc gccgccggcc gacaggctca gggtggtggc gttgaacacg
     5821 gcgccggccg ccgacggggc ggcggtctgg gcgagcgcgc cgcctgccga tccggcgacg
     5881 atcagggctc cgagggcggc ggcgcggaag agggctttca tggcgggtct ccggacgggc
     5941 ggggttgctg gcgcctgtcg cccggaccat agcctgatcc gacatgaacc ggacctttaa
     6001 gcggcgttcg gcccgtggta accgcgcaga cagccccggg ggcccgtagc tcaatggtta
     6061 gagccgaccg ctcataacgg tctggttgca ggttcgagtc ctgccgggcc taccatcgcg
     6121 ccggtcagac ggcgttgagc gggatcttca ggtaccgcac gccgttgtct tccggttcgg
     6181 gcagatggcc ggcgcgcatg ttgacctgga ccgacggcag gatcaggcgc ggcatgtcca
     6241 gcgtccggtc gcgcgcctcg cgcagggcga cgaacgcctc ctgcgtcacg ccctcatgaa
     6301 catggacgtt gctgcggcgc tgttcgccga tggtcgtttc ccacaggtac cggtcgcgtc
     6361 cgggcgcctt gtagtcatgg cagaggaaca cccgggtgtc cgccggcagg ctcagcagcc
     6421 gctggatcga ccggtacagc gtcgccgcgt cgccgcccgg aaagtcgcag cgggcggtgc
     6481 cgtagtcggg catgaacagg gtgtcgccga caaacaccgc gtccccgatc acataggcca
     6541 tgcaggcggg cgtgtgacct ggcacatgca gcgccacggc ctgcagcccg ccgatggcga
     6601 accgctctcc gtccccgaac agatggtcga actgccgacc gtcgcgggcg aagtcgggac
     6661 cggcgttgaa cagcgcgccg aacgtctcct gcaccctctg gatgcggtcg ccgatcgcca
     6721 gtcgcccgcc gagggcctcc tgcaggtagg gcgcggccga cagatggtcg gcgtgggcat
     6781 gggtttccag ctgccagacc acctcgagcc cttcggcccg gacgtggtcg atcaccgcgt
     6841 cggccgagcg ggacgccgtg cggccggacg ccggatcgaa gtcgagcacg ctgtcgatga
     6901 tggcgcaggc cgtggacccg ggatcgcgca ccacatagct gacggtgaag gtcgcctcgt
     6961 cgaagaaggc cttgacctgc gcctgccgcg cgggatcggc gcgggccgcg tcgatgatgc
     7021 cggtggccac ggccagcggg gcgtcggcgg acagggtcgg gtcggacatt ggaaaactcg
     7081 cgggttgcat cgttcgtcga taacatatat gcgaatatat gcatatacaa gccccggcgg
     7141 gatggccatg accctgatca caattctctc cgccctgatg agcggggccg tcgtcggcgc
     7201 cctgctggcg ttgttcggcg gtggcgggtc ggtgctggcc acgccgttgc tgctctacgt
     7261 cgtcggcgtg cgggacccgc acgtggcgat cggcacctcc gccgccgcgg tggcggtcaa
     7321 cgccctgatc aatcttgtcg ggcatggtcg ggaagggcgg gtcaaatggc cttgcgccct
     7381 ggtgttcgcg gccgccggcc tggccggatc gttcctcggc tcgaccctgg ccaagcaggt
     7441 cgatggtcag cggctgctgc tgttcttcgc catggccatg gcggccatcg ccctgtcgat
     7501 gctgcgccgc ccgcggtcgg aaggcgatcc ggcggtgcgc atcacctggc ggttcaccct
     7561 gcgcctcgcg cccctgggtc tgctgaccgg cctggcggcc ggtttcttcg gcatcggcgg
     7621 cggcttcctg atcgtgccgg gactgatggc ggcgaccggc atgaccctgg ccaacgcggc
     7681 cgcgtcgtcg ctgttgtcgg tggcgttgtt cggcgcggcg acctcggcca actacgccct
     7741 gtcgggcctg gtcgactggc cgatcgccgg cctgttcatc ctgggcggcg ccgtcggcgg
     7801 atgggtcgga cttcggggcg caggcctgct ggccggccgt gcgttgctgg gccgtcggct
     7861 gttcgccggc ctggtgctga tcgtggccgg ttacgtcgcc tggcgggccg tgaccggcta
     7921 acggtcaacc gcccggatcg tctccgcgca cagggtcagc ggcagtggcc ggtgactgac
     7981 gatgatgacg ccctggccgg tgcgggacag ccgccggtcg agcgcggcca ccaccacggc
     8041 ctcggtggcg ctgtcgaggc cctcggttgg ctcgtcgagc aacagccacg gcgccggcct
     8101 caggtaggct cgcgccagcg aca
//
LOCUS       DAORNX010000078         8090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6565_length_8090_cov_7.478034, whole genome shotgun sequence.
ACCESSION   DAORNX010000078 DAORNX010000000
VERSION     DAORNX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_8090_cov_7.478034"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PLO65_05080"
     CDS             <1..1258
                     /locus_tag="PLO65_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRD27655.1"
                     /translation="VVARGAGIPCVCGATDIRVDAEAGQFTVRGRTFREGDLITIDGA
                     TGEVMAGETPMIEPELGGDFDTLMSWADGIRRLKVRANAETPNDGRTARQFGAEGVGL
                     CRTEHMFFNEDRITAVREMILADDEQGRRAALATIAPFQKADFVELFTIMEGLPVTIR
                     LLDPPLHEFFPAHADDLKAVAETLGLSLDAIGQRLQVLHESNPMLGHRGCRLGISYPE
                     IYEMQVRAIMEAACEIARSGKPAPVPEIMHPLVGKGEEMAFLRKLTDRTARAVLEEQG
                     LDLAYQVGTMIELPRAALRAADLAAAGAEFFSFGTNDLTQTTFGISRDDAGKFLGAYL
                     EKGIFEQDPFVTLDQDGVGDLIRLAAERGRTVNPGLKLGICGEHGGDPASIAFCESVG
                     LDYVSCSPFRVPIARLAAAQAVLNRK"
     gene            1416..1973
                     /locus_tag="PLO65_05085"
     CDS             1416..1973
                     /locus_tag="PLO65_05085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27656.1"
                     /translation="MTRLVTPDREHLPAYVAALEQGWSPDNVLDKAAADAQLVRIAQD
                     ADAFLAGLDDPEARGGPIPQPDGSFTERLPGFHRWIWDDGFCGSIGFRWRRGTEDLPP
                     TCLGHVGYAVVPWKQGRGHATAALALILPQARAVGLRWIDLTTDPANLASQRVILANG
                     GALLERFVKPAVFGGAEGLRYRIPL"
     gene            complement(1980..2681)
                     /locus_tag="PLO65_05090"
     CDS             complement(1980..2681)
                     /locus_tag="PLO65_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HRD27657.1"
                     /translation="MSRLRTHAERHLVARIGWLRAAVLGANDGIVSTASLIVGVAAAA
                     GGRSEVLIAGMAGLVAGAMSMAAGEYVSVSSQSDTERADLARETAELASDPEAEHREL
                     AAIYVSRGLTPDLAAQVATQLMSGDALAAHARDELGISEITQARPIQAALTSAGTFSV
                     GAAMPLLMVVLVPPASLAVAVSVASLAFLALLGAIGAKAGGANLWKATARVTFWGALA
                     MAVTAGIGKLFGVMV"
     gene            complement(2678..2962)
                     /locus_tag="PLO65_05095"
     CDS             complement(2678..2962)
                     /locus_tag="PLO65_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HRD27658.1"
                     /translation="MASTLVETIGFAAAATSTLCWFPQSVRTLRTRDTAGISLISQAA
                     FTGGVLLWASYGVLIGSLPLILCNTIQLVPLAAILWIKVGNERSTRELSS"
     gene            complement(3060..3992)
                     /locus_tag="PLO65_05100"
     CDS             complement(3060..3992)
                     /locus_tag="PLO65_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27659.1"
                     /translation="MNLRHAVRVNIRLNLDVIRATRAALGGDLERSLIFAAISAANVA
                     ILEDDPALSLQLAHTSVPDPLRRPIRVQRIAESLGLPRETTRVKVRQLLAEGLLEETP
                     AGLLMSADTARSPRFEPMFGDYIAALARAIERLAAGGCVGLTASDRLAGPPLPATWGA
                     IRLVTQHILRGVVELRASVGPTSLIKAYLYLAMADGTAGHFSQGEVLRHADFDDPPPP
                     AARRTISASSLALSLDLPRETVRRNLQALAREGYLFQRSGGFGIPTPVSAPDLQRERE
                     VYQRSMADLSRLVRRLRQIDAITGQGAVIPSPAP"
     gene            complement(3989..4381)
                     /locus_tag="PLO65_05105"
     CDS             complement(3989..4381)
                     /locus_tag="PLO65_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27660.1"
                     /translation="MSNVVAYPAGGRRSPARGALRVNPLLAEIVDALAYFHGHAHRDA
                     VCNLIASRRSGAPAIASSVLRDEIRAAFSAHLDPVAGSGRQRSLIRLAFGEGSHRWSL
                     SEDGLRLLFDARAAMDPAPGRRPDLPEA"
     gene            4583..5833
                     /locus_tag="PLO65_05110"
     CDS             4583..5833
                     /locus_tag="PLO65_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRD27661.1"
                     /translation="MAAGSTTFDAAAETAKYLATLSPEAHARATAYTQGGHWLLLWGT
                     LVAILVSWIIIRTGVLPRIRDGAGKGGIGWLAALAVLPVWFVMDSLLSLPWALYTDWW
                     RQTQYGLTSQALGGWFADWGKGLGVGLVMTVILFSLIYWLMRLSPRRWWLWGGLVTSA
                     FLIFGMVIAPVLIEPLFNKYTPAPPGPVRETVVAMAQANGVPSDKIFIYNGSKQSNAY
                     TANVSGLFGSARVAMSDTMFKKGADIAEVRGVVGHEMGHYVHMHALWFAGVFSVVAML
                     GFWLANRLFPLAARLMGSGVETIADPAGLPVLMTVLSVLSLLGTPLINTVVRTAEADA
                     DNFSLQRVNEPDGLAKALVKTIEYRAATPGRWEEIIFYDHPSVGWRVRNAMDWKAAHL
                     KPEPATVNVDMIELPPADAGALTK"
     gene            complement(5941..7056)
                     /locus_tag="PLO65_05115"
     CDS             complement(5941..7056)
                     /locus_tag="PLO65_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRD27662.1"
                     /translation="MHQAVIAATGLYTPEQSISNTELVEAFNGYVERFNAANAGAIAA
                     GQLEALTPSSADFIEKASGIKARFVMNKSGILDPEIMRPVIPERPNEEISILAEIAVA
                     AAREAIARWGKPVSEIGAVLCAASNMQRAYPAMAIEVQQALGIEGFAFDMNVACSSAT
                     FGIKTAADFIAGGSAKAVLMVNPEICSGHLNFKDRDSHFIFGDVATAVIVEAADQATG
                     GWEILGTKLKTQFSNNIRNNFGFLNNAAPEGIGAKDKLFVQEGRKVFREVVPMVSQMI
                     VDHAAELGLDPTGLKRLWLHQANINMNEMIGRRVLGRDPTPEENVIILDTYANTSSAG
                     SIIAFHRASDDFAAGDTGLICSFGAGYSAGTVFVRKR"
     gene            7276..7770
                     /locus_tag="PLO65_05120"
     CDS             7276..7770
                     /locus_tag="PLO65_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRD27663.1"
                     /translation="MKSLMIAAATTALSVAAVPAVSQAQEAYGTIGYANVDADDANIG
                     AIQGRLGYRFNPYVGVEGEAAFGVNGDTIGGVDVDLQHEVGAYVVGFVPVTPRADLFA
                     RVGYTSSSFDTSLGDVDGDGVAWGVGGQYHFTDKDGVRLDWTRHDYDAGDADVWALAY
                     TRKF"
     gene            7882..>8090
                     /locus_tag="PLO65_05125"
     CDS             7882..>8090
                     /locus_tag="PLO65_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27664.1"
                     /translation="MNATIIQLAVSGALVGLMIAVAGWARIARPVAPLDEATARAVLA
                     AEFPDLTADLLWIAGDGAGAVARAGG"
BASE COUNT         1213 a         2788 c         2857 g         1232 t
ORIGIN      
        1 ggtggtggcg cgcggcgcgg ggattccctg cgtctgcggc gcgaccgaca tccgcgtcga
       61 cgccgaggcc ggccagttca ccgtgcgcgg ccggaccttc cgcgaaggcg acctgatcac
      121 catcgacggc gccacgggcg aggtgatggc cggcgagacg ccgatgatcg agcccgagct
      181 cggcggcgac ttcgacaccc tgatgagctg ggccgacggc atccgtcgcc tgaaggtccg
      241 ggccaacgcc gagaccccca acgacggccg cacggcccgc cagttcggcg ccgagggtgt
      301 cggcctgtgc cgcaccgagc acatgttctt caacgaggac cggatcaccg cggtgcgcga
      361 gatgatcctg gccgacgacg aacagggccg ccgcgccgcc ttggcgacca tcgccccgtt
      421 ccagaaggcc gacttcgtcg aactgttcac catcatggaa ggcctgccgg tgaccatccg
      481 cctgctcgac ccgccgctgc acgagttctt cccggcccac gcggacgacc tgaaggcggt
      541 ggccgagacg ctcggcctgt cgctcgacgc catcgggcag cggctgcagg tgttgcacga
      601 gtccaacccg atgctcggcc accgcggctg ccggctcggc atcagctatc ccgagatcta
      661 cgagatgcag gtccgggcga tcatggaggc ggcctgcgag atcgcccggt cgggcaagcc
      721 cgcccccgtg cccgagatca tgcacccgct ggtcggcaag ggcgaggaga tggcattcct
      781 gcgcaagctg accgaccgca cggcccgggc ggtgctggaa gaacaggggc tggacctggc
      841 ctaccaggtc ggcacgatga tcgagctgcc gcgcgcggcc ctgcgcgccg ccgacctcgc
      901 cgcggcgggc gccgagttct tcagcttcgg caccaacgac ctgacccaga ccaccttcgg
      961 catcagccgc gacgacgccg gcaagttcct cggcgcctat ctggaaaagg gcatcttcga
     1021 acaggacccg ttcgtcaccc tcgaccagga cggcgtcggc gacctgatcc ggctggcggc
     1081 cgaacggggc cgcacggtca atccgggtct caagctgggc atctgcggcg agcacggcgg
     1141 tgaccccgcg tcgatcgcct tctgcgagtc ggtgggtctc gactacgtca gctgctcccc
     1201 cttccgcgtg ccgatcgccc ggctggcggc cgcccaggcc gtgctgaaca ggaagtagga
     1261 ggcaggttgc gtggttcgac acgcgccttc ggcgctgctc accatgacgt gcaccgagct
     1321 acgtcatcct gagcggccgc gcggcggcgc atcgaaggac gcaggccgct gaaccgccac
     1381 cttgtcccga cctgccgggc tgctagcctt gcttgatgac ccgcctcgtc acccccgacc
     1441 gcgagcacct gccggcctac gtcgcggcgc tcgaacaggg ctggtcgcct gacaatgtgc
     1501 tggacaaggc cgccgccgac gcccagctgg tccggatcgc ccaggacgcc gacgcgtttc
     1561 tggccggcct cgacgatccg gaggcgcggg gcggtcccat cccgcagccg gacggcagct
     1621 tcaccgagcg cctgccggga tttcaccgct ggatctggga cgatggcttc tgcggttcga
     1681 tcggctttcg ctggcgccgc gggaccgagg atcttccgcc gacctgcctg ggccacgtcg
     1741 gctatgccgt cgtcccgtgg aagcagggcc ggggccacgc cacggccgct ctggcgctga
     1801 tcctgccgca ggcccgggcg gtcggcctgc gctggatcga cctgaccacc gacccggcca
     1861 acctggcctc gcagcgagtg atcctggcca acggcggcgc gctgctggag cggttcgtca
     1921 agccggctgt cttcggcggc gcggagggcc tgcgctaccg catcccgctg tagaccgcgc
     1981 tacaccatca ccccgaacag cttgccgatc ccggcggtga cggccatggc cagggcgccc
     2041 cagaaggtca cccgggcggt cgccttccac aggttcgcgc cgccggcctt cgccccgatc
     2101 gccccgagca gggccaggaa ggccagcgag gcgacggaca ccgccaccgc cagactggcc
     2161 ggcggaacca ggaccaccat cagcaggggc atggccgcgc cgaccgagaa ggtccccgca
     2221 gaggtcaggg ccgcctggat cggccgggcc tgggtgatct cggagatgcc cagctcgtcg
     2281 cgggcatggg cggccagggc gtcgcccgac atcagctggg tcgccacctg ggccgccagg
     2341 tccggcgtaa ggccgcgact gacatagatg gccgccagtt cgcggtgctc ggcctccgga
     2401 tcgctggcca gttcggccgt ctcccgcgcc aggtccgccc gttcggtgtc ggactgcgag
     2461 ctgaccgaga cgtattcgcc ggcggccatc gacatggccc ccgcgacgag gccggccatg
     2521 ccggcgatca gcacctcgct ccggccgccg gccgccgcgg cgacgccgac gatcaggctg
     2581 gcggttgaga cgatcccgtc attggccccc agcacggccg cgcgcagcca gccgatccgg
     2641 gccaccaggt ggcgttcggc gtgggttctc aggcggctca tgaggacagc tcccgggtag
     2701 agcgttcatt gccgaccttg atccacagga tcgccgccag cgggaccagc tggatggtgt
     2761 tgcacaggat cagcggcagg ctgccgatca gcacgccgta cgacgcccac agcaggacgc
     2821 cgccggtgaa ggccgcctgg gagatcagcg agatgccggc ggtgtcgcgg gtccgcaggg
     2881 tccggaccga ctggggaaac cagcacaggg tcgaggtcgc ggcggcggcg aaaccaatgg
     2941 tttccacgag ggtcgaggcc aaggatcggg tctccggacc gggacggccg atcccatgat
     3001 cggccgagtc ggcctgcctg tgtcatccct gtccaaaggc ggcgcggacg ggcgcgcctt
     3061 cagggcgccg gggagggaat cacggcgccc tggcccgtga tggcgtcgat ctggcgtagc
     3121 cgccgcacca gtcgactgag gtcggccatg ctgcgctgat agacctctcg ctcccgttgc
     3181 aggtccggcg cggagaccgg tgtcggaatg ccaaatccgc cggaccgctg gaaaagatag
     3241 ccctcgcggg cgagcgcctg gagattgcgg cgcacggtct cgcgcggcag gtccagcgac
     3301 agcgccaggc tgctggcgct gatcgtccgg cgggccgcgg gcggcggcgg atcgtcgaag
     3361 tcggcatgac ggagaacctc gccctggctg aaatggccgg cggtcccgtc ggccatggcg
     3421 aggtacagat aggccttgat caggctcgtc ggaccgaccg acgcccgcag ctcgacgacc
     3481 ccgcgaagaa tgtgctgggt gaccaggcgg atggcgcccc aggtcgcggg aagcggcggt
     3541 ccggcgagcc ggtcggacgc cgtcaggccg acgcacccgc cagcggccag ccgttcaatc
     3601 gcccgggcca gcgcggcaat gtagtcgccg aacatcggct cgaagcgggg cgagcgggcc
     3661 gtgtcagccg acatcagcag gccggcggga gtctcctcca gcaagccctc ggccagcagt
     3721 tgccggacct tcacgcgggt ggtttcccgg gggaggccga gcgactcggc gatccgctgg
     3781 acccggatgg gccgccggag ggggtcggga acactggtgt gggccagctg caggctgagc
     3841 gccgggtcat cctccagaat ggcgacattg gcggcggaga tcgccgcgaa gatcaggctg
     3901 cgttcgaggt ctccgccgag cgccgcccgt gtcgcccgga tgacgtccag gttcagccgg
     3961 atgttcaccc ggacagcgtg gcggaggttc atgcctcggg caggtccggg cggcggccgg
     4021 gggccggatc catggcggcg cgcgcgtcga acagcagtcg cagtccgtcc tcggacaggc
     4081 tccaccggtg gctgccttcg ccgaacgcca ggcggatcaa cgaccgctgt cgaccggagc
     4141 cggccaccgg gtcgagatgg gcggagaagg cggcgcggat ctcgtccctg aggacgctgg
     4201 aggcgatcgc gggcgcgccg gagcgccggg aggcgatcag attgcagacg gcgtcgcgat
     4261 gggcgtgccc atgaaagtac gccagggcgt cgacgatttc ggccaggagc gggttgaccc
     4321 ggagggcccc acgcgccggc gaccgtctgc ccccggctgg ataggcgacg acattcgaca
     4381 tcgagacgtc ccgtgggtaa cgatgttcac ctaacggcgg ggggcggcct gtccagtccg
     4441 ctggcgccag aaatgggcgc cccctcgccc gggcggacaa tatgccgcgg cgccgggccc
     4501 gccgtcgctg atcgcaattt cacctgacaa tggtcggcgt ccatggccat gctgccgcct
     4561 ggtttgggga ggagggcgcg ccatggccgc cggttcaacg acgttcgacg ccgcggcgga
     4621 gaccgcgaaa tacctggcga cgctgtcgcc cgaggcgcat gccagggcca cggcctacac
     4681 ccagggcggc cactggctgc tgctgtgggg gaccctggtc gctatcctgg tgtcctggat
     4741 cattatccgc acgggcgttc ttccccgcat ccgcgacggc gccggaaaag gcgggatcgg
     4801 ctggctggcg gcgctggccg tcctgccggt ctggttcgtg atggactcgc tgctcagcct
     4861 gccctgggcg ctctacaccg actggtggcg gcagacccag tacggcctga ccagccaggc
     4921 cctgggcggc tggttcgccg actggggcaa gggcctgggc gtcggcctgg tcatgacggt
     4981 catcctgttc agcctgatct actggctcat gcgcctgtca cccagacgct ggtggctgtg
     5041 gggcggcctg gtgacgagcg ccttcctgat cttcggcatg gtcatcgcac cggtgctgat
     5101 cgagccgctg ttcaacaaat acacgcccgc gccgcccggg ccggtgcgcg agacggtcgt
     5161 ggccatggcc caggccaacg gcgtgccgtc cgacaagatc ttcatctaca acggctcgaa
     5221 acagtcgaac gcctacacgg ccaatgtctc gggcctgttc ggctcggccc gcgtggccat
     5281 gagcgacacc atgttcaaga agggcgcgga catcgccgag gtgcgcggcg tggtcggcca
     5341 cgagatgggc cactatgtcc acatgcacgc cctgtggttc gcgggcgtgt tcagcgtcgt
     5401 cgccatgctg ggcttctggc tggcgaaccg gctgttcccg ctggccgccc gcctgatggg
     5461 gtcgggcgtg gaaaccatcg ccgatccggc cggcctgccc gtgctgatga cggtgctttc
     5521 ggtgctgagc cttctgggca cgccgttgat caacaccgtg gtccggaccg ccgaggccga
     5581 tgccgacaac ttctcgctcc agcgcgtcaa cgagcccgac ggcctggcca aggcgctggt
     5641 caagaccatc gagtaccgcg ccgccacgcc cggcagatgg gaggagatca tcttctacga
     5701 ccacccgtcg gtcggctggc gcgtgcgcaa cgccatggac tggaaggcgg cgcacctgaa
     5761 gcctgagccg gcgacggtga atgtggatat gattgagctt ccgccggcgg acgcgggtgc
     5821 gctgacgaag tagcattgcg tccttcgaca cggcccttcg ggcctgctca ggatgacgtc
     5881 ctcaggtgca cgtcatggtg agcagcgccc gaagggggcg tgtcgaacca cgcaggtcga
     5941 ctaccgcttt cgcacgaaca ccgtgcccgc cgaatagccc gcgccgaagc tgcagatcag
     6001 gcccgtgtcg ccggcggcga agtcgtcgct ggcccggtgg aaggcgatga tcgagccggc
     6061 cgaggaggtg ttggcgtagg tgtcgaggat gatcacgttc tcctcgggcg tcgggtcgcg
     6121 gcccagcacc cggcgtccga tcatctcgtt catgttgata ttggcctggt gcagccagag
     6181 gcgcttgagc ccggtcgggt cgaggcccag ctcggcggca tgatcgacga tcatctggct
     6241 gaccatcggc accacctcgc ggaacacctt gcggccctcc tgcacgaaca gcttgtcctt
     6301 cgccccgatc ccctccggcg cggcgttgtt gaggaagccg aagttgttgc ggatgttgtt
     6361 ggagaactgc gtcttcagct tcgtgccgag gatttcccag ccgccggtcg cctggtcggc
     6421 ggcctcgacg atcaccgccg tggccacgtc gccgaagatg aagtggctgt cgcggtcctt
     6481 gaagttcaaa tggcccgagc agatctccgg attgaccatc agcacggcct tcgccgatcc
     6541 gccggcgatg aagtcggcgg ccgtcttgat gccgaaggtg gcgctggaac aggccacgtt
     6601 catgtcgaag gcgaagccct cgatgcccag cgcctgctgc acctcgatgg ccatggccgg
     6661 gtaggcccgc tgcatgtttg aggcggcgca gagcacggcg ccgatctcgc tgaccggctt
     6721 gccccatctg gcgatcgcct cgcgggcggc ggccaccgcg atctcggcga ggatcgagat
     6781 ctcctcgttg ggccgctcgg gaatcaccgg ccgcatgatc tccgggtcga ggatgcctga
     6841 cttgttcatc acgaagcggg ccttgatgcc cgaggccttc tcgatgaagt cggcggagga
     6901 gggggtcagg gcctcgagct ggccggcggc gatcgccccg gcgttggccg cgttgaaccg
     6961 ttcgacgtag ccgttgaagg cctcgaccag ctcggtgttg gagatgctct gttcgggcgt
     7021 gtagagcccg gtggcggcga tgacggcctg atgcacgatt ggggtcccga cgatggtcgc
     7081 gcctgcctga tgcgcggcgc tgttctgttc gcggcgatag atagcacgcc cggcggccgc
     7141 gtcagcccat gtggccggac ggcaacgcta caaacccgcc tcattccgtc cgttgaaccg
     7201 ccgcggacgc gccgcgtttg atccctacga acagggcggc tggggagctg gaaaagcgtc
     7261 aaggagtcca agacgatgaa atctctcatg attgccgccg cgaccaccgc cctgtcggtc
     7321 gccgccgtcc ccgcggtctc gcaggcccag gaggcctacg gcacgatcgg ctacgccaat
     7381 gtcgatgccg atgacgccaa catcggcgcc atccagggtc gcctcggcta caggttcaat
     7441 ccctacgtcg gcgtcgaggg cgaagcggcc ttcggcgtca acggcgacac catcggcggt
     7501 gtcgatgtcg acctccagca tgaggtgggc gcctatgtcg tcggcttcgt gccggtgacc
     7561 ccgcgagctg acctgttcgc gcgcgtcggc tacaccagct cgagcttcga tacctcgctg
     7621 ggcgacgtgg acggcgatgg cgtcgcctgg ggagtcggcg gccagtatca cttcaccgac
     7681 aaggacggcg tgcgcctcga ctggacgcgt cacgactatg acgccggcga cgccgacgtc
     7741 tgggccctgg cctacacccg caagttctag gtccggccgc ttccgcccga cctgcaggcc
     7801 cctcccggcg acgggagggg ctttttggtt gacgccgcgc ccccggcgac cacactcccc
     7861 ggccggggag aaggcgcgca cgtgaacgca accatcatac agctggccgt gtccggggcc
     7921 ctggtcgggc tgatgatcgc cgtggccggc tgggcgcgca tcgcccggcc cgtcgcgccg
     7981 cttgacgagg ccaccgcccg cgcggtgctg gccgccgagt ttccggacct gacggctgac
     8041 ctgctgtgga ttgccggaga cggcgccggg gccgtcgcgc gggccggcgg
//
LOCUS       DAORNX010000079         8068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6586_length_8068_cov_6.938725, whole genome shotgun sequence.
ACCESSION   DAORNX010000079 DAORNX010000000
VERSION     DAORNX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_8068_cov_6.938725"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..127)
                     /locus_tag="PLO65_05130"
                     /pseudo
     CDS             complement(1..127)
                     /locus_tag="PLO65_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(239..436)
                     /locus_tag="PLO65_05135"
     CDS             complement(239..436)
                     /locus_tag="PLO65_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD27665.1"
                     /translation="MKNRLKVLRAERDWSQADLAERLEVSRQTINALETGKYDPSLPL
                     AFRIARLFGQPIEAIFDDGQA"
     gene            complement(433..831)
                     /locus_tag="PLO65_05140"
     CDS             complement(433..831)
                     /locus_tag="PLO65_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27666.1"
                     /translation="MNKHSPAGRRYLKRFIPAMVAYLVLIFGASYAFRDPGLTGPLAW
                     AIAVAPALPIVAVIAIMGLYIKEETDEFQRRILIESMLWGFGVTLTVTTVSGFLEMYV
                     HTPGLQSFWAFPIFCLAMGVSQLFVRRRYR"
     gene            1014..1496
                     /locus_tag="PLO65_05145"
     CDS             1014..1496
                     /locus_tag="PLO65_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27667.1"
                     /translation="MIQEIDADVAGRGLYVSNYLSPAGATQWPTLLREAARSGTDDTL
                     AGQLSINRCFKAQVERRKPKGGTTWVAVPNTAPMTLAEGQFNTYYMRALAIRAIAAGA
                     GLLVYRAKAVDAPRTNSEQMIGTVLDPQTVLDALRATLGVEPVIGIPLPNSGLCVRLR
                     "
     gene            1738..1968
                     /locus_tag="PLO65_05150"
     CDS             1738..1968
                     /locus_tag="PLO65_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27668.1"
                     /translation="MFKIGDSFSQKFDLKLSRLGPLIEPRPYEEGDDAKDERSNLADW
                     FRAKNCSQREPYATDYDEQNNLAPSRLEQPLS"
     gene            complement(1974..2123)
                     /locus_tag="PLO65_05155"
     CDS             complement(1974..2123)
                     /locus_tag="PLO65_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27669.1"
                     /translation="MTEPKHDTTQADKFRDLARELECDEDEGAFEATVKKVAKAPKPE
                     KADSE"
     gene            2122..2865
                     /locus_tag="PLO65_05160"
     CDS             2122..2865
                     /locus_tag="PLO65_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRD27670.1"
                     /translation="MALLPKPEIVAFIDESGDFELAKIDPNYPVCAQCALTCTVDEYL
                     AKAVPNLMNLKYHFFGNEAVVMHGHKIRKRSLPFDILKDDEIRAEFMDAVSWAIGQLE
                     GCLIVAAVHKPRHVGQYIHPTDPFFLSLQFLLERLYMHWVGKLVGGRRLLCVFEKRGP
                     KEDERTRNWFHQICDGDNYRAQRFPFDIDFRSKDDNVIGHQYADLVAYAACRFVETGD
                     GERRDWMAVKGKLRTVDGRYETHGLKVFP"
     gene            complement(3050..4018)
                     /locus_tag="PLO65_05165"
     CDS             complement(3050..4018)
                     /locus_tag="PLO65_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRD27671.1"
                     /translation="MSVLSKPYFHDEAAAFAFVEGILWGSEPVCPHCGCTGRVYALAN
                     VRGSKSKANPEGAIRHGLKKCGDCRKQFTVRVGTLFEESHLPMTKWLQAIYLLCSSKK
                     GISAKQLERTLETTYKTAWFLAHRIREAMRSGELAPFGQGGGSVEVDETYLGIDPDKP
                     KSRTTIRNMNMVMTLIDRQTGRARSLVVDHLNAATVTEILNANLAREARLITDEATSY
                     ITPGKAFADHKSVRHARKEYVSQADRTVHTNTVEGFYSIFKRGMRGVYQHASKKHLHR
                     YAAEFDFRYSNRIALGVDDTKRAERALQGVVGKRLTYRSTDSRTQA"
     gene            complement(4133..5149)
                     /locus_tag="PLO65_05170"
     CDS             complement(4133..5149)
                     /locus_tag="PLO65_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HRD27672.1"
                     /translation="MNDAHAAGILPCQSIEDLIAREAIGSLTPFDSDQVQPASLDLRL
                     GERCWRVRASFLPGRRRVLDRLPDVAMHELDLTRGAVLERGCVYIAEVQERLALPPGV
                     SARGNPKSSSGRVDVFVRLLTDHGPAFDDVAEGYTGPLYIEIAPQTFSVLVRAGTRLN
                     QLRLKRGTPVRLSSRDVGVDLSIGTVGFRGRRHAGVIDLDREDGHDPRDFWEMLESPK
                     GELLLDPNEFYILASKDDIEIPVLEAAEMTPIDPAVGEFRVHYAGFFDPGFGTDETHS
                     RGSKGVLEVRSHETPFLLEDGQTVARLVYEPLTARPTRLYGEDGSHYLRQGLKLSKHF
                     RPWK"
     gene            complement(5215..5424)
                     /locus_tag="PLO65_05175"
     CDS             complement(5215..5424)
                     /locus_tag="PLO65_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27673.1"
                     /translation="MLAMVFRVFAAILGVLGAINVVLLVLGEALARYSPVELHAVGTL
                     TEALMLFGVGAGIYVLSPAGGKAHH"
     gene            5660..6844
                     /gene="metZ"
                     /locus_tag="PLO65_05180"
     CDS             5660..6844
                     /gene="metZ"
                     /locus_tag="PLO65_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRD27674.1"
                     /translation="MAEDPHDWQPATRLIRGGVARSNYGEISEALYLTQSFAYESAGA
                     ADRRFSGDDPGFVYSRYANPTTQMFEERLALLEGAEYCRALASGMAAVHTALTGLLRA
                     GDHLVAGRALFGSCNWIVQTWLPRFGVETTLVDATDLGAWEAAMRPNTKVVLLESPAN
                     PLLEITDIAAVAKIAHAAGARVVVDNVFATPIFQKPLALGADVVVYSATKHIDGQGRV
                     LGGAILGGKALLEEAYRDIIRHTGPAISPFNAWILLKGLETLDLRVRRQTDSAAALAD
                     QIGASPKVRRVLYPGRADHPQAAVARAQMSGFSNLLAFDLGSREAAWRFLDALEIFDI
                     SNNLGDAKSMATHPSTTTHRAMPEANRLEIGLTEGYVRMSVGLEDVRDLARDVQRALD
                     AA"
     gene            6870..7301
                     /gene="apaG"
                     /locus_tag="PLO65_05185"
     CDS             6870..7301
                     /gene="apaG"
                     /locus_tag="PLO65_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRD27675.1"
                     /translation="MSRINRRGTPDSPVYEARTGDILVRVAPVYLPEESNPAGGRWIW
                     GYTVEIENHGRDTVTLVSRHWIITDGRNRIEEVRGAGVVGEQPVLGPREAFRYQSGCP
                     LGTPSGAMRGTYQMVTAAGEAFEVTIPEFSLHLPGARRRLN"
     gene            complement(7309..>8068)
                     /locus_tag="PLO65_05190"
     CDS             complement(7309..>8068)
                     /locus_tag="PLO65_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27676.1"
                     /translation="GGGVLGGALFGEVGVWAYLAFTGVLLFAAAFVAGAPDRPSRDLR
                     APPSDALGQLRAFTVALLDRDFGLVWVSKVLIMFAYSAATTFTLYMLQSYIQPALGAL
                     EAARITPLLGLAALPGTMVAMAVCGRWSDRIGRRKPFVIASSLLFAVALAVPLVSPTL
                     PALFVQAVLIGLALGCFMVVDQALMIDVLPDQAAAGRDLGIGILAINLGQAIAPAVAG
                     GIVAATGDYTLIWVVGVVTVLVATAAILPVKRAR"
BASE COUNT         1438 a         2632 c         2639 g         1359 t
ORIGIN      
        1 ggaccagatg cacgcggtag cggccctcga gccgggcggc cgtgtcgcgc cagaccgccg
       61 ccgacgaggt caggccgggg atcaggatca cgtcgggccc ctgcccgcgc acctcgacgg
      121 agaatcgcgt gggcccggcg tgcgccgcgc cggcgcccag cggcaggaac agacccagca
      181 gggcgaggac gcgtttcatt gacctgaaca tggcgagtct ccgtgacggg cgtgcggctc
      241 aggcctggcc gtcgtcgaaa atggcttcga tgggctggcc gaacagccgg gcgatgcgga
      301 aggccagcgg caggctgggg tcgtacttgc ccgtctccag cgcgttgatg gtctggcgcg
      361 agacctcgag ccgctcggcc aggtccgcct ggctccagtc gcgctcggcc cgcaggacct
      421 tgaggcggtt cttcaccggt agcgcctccg cacgaacagc tgcgagacgc ccatggccag
      481 gcagaagatc ggaaaggccc agaacgactg cagccccggc gtgtggacgt acatctccag
      541 gaagcccgag acggtggtca ccgtcagggt gacgccaaag ccccagagca tcgactcgat
      601 caggatgcgg cgctggaact cgtcggtctc ctccttgatg tagagtccca tgatggcgat
      661 caccgccacg atcggcaggg ccggcgccac ggcgatcgcc caggccagtg gccccgtcag
      721 gcccggatcg cggaaggcgt aggacgcgcc gaagatcagc acgagatagg cgaccatcgc
      781 cgggatgaag cgcttgaggt agcgccggcc ggccgggctg tgcttgttca cggaaagact
      841 ccttgtctgg atgacaagga tacttgtctc atccaggcat ggatcgtgtc aaggacgccg
      901 gcgattatgc ctttcgcaaa cccagcgact cagggtagca agagcgttcg ttttcaacgg
      961 gcgcgaacat ggctctacaa ttccatcacc tcgacgaaga gacgcgtggc ttgatgatcc
     1021 aagagatcga cgccgatgtg gctggtcgtg gtctctacgt aagcaactac ctttctcccg
     1081 caggcgctac tcagtggccg accctgctgc gtgaagcggc ccgctccggg accgacgaca
     1141 cactcgcggg ccaactctcg atcaacaggt gcttcaaagc ccaggttgag cgccgcaagc
     1201 ccaagggcgg tacgacgtgg gtggcagttc caaacaccgc gccgatgacc ttagccgaag
     1261 ggcagttcaa cacttactac atgagggctc tggccatacg ggcaatagcc gctggagcag
     1321 gcctgcttgt ctaccgggcc aaagctgttg atgctccacg gacaaattca gaacagatga
     1381 tcgggacggt gctagacccg cagaccgtgt tggatgcttt gcgggcaacg ttgggcgttg
     1441 agcccgtcat tggaatcccc ttgccgaact ctgggctgtg cgtccgtcta cgctaggacg
     1501 agcctgcctt ggtcgttaca ggtccatgaa aatagcaagt catccggttc catggcgtag
     1561 agttcagggg tgccgtttat tcccattcga taccgccggg tcagtgccgt aagctccgaa
     1621 aggaacaggg tcattaggga gccctcgcta ggctcggcct gtgcaatttc gctgatcatg
     1681 cggtatcata ataccgcagc aagggaatgt gaacccctgc ggttagtcgc cggcccggtg
     1741 ttcaagatcg gcgactcgtt ctctcaaaag ttcgatctca aactcagccg tctcggcccg
     1801 ctgatcgagc cgcgcccata tgaagaaggc gatgacgcca aagacgaacg gagcaatctt
     1861 gccgattggt tccgggccaa aaattgctct cagcgcgagc cctacgctac cgactacgac
     1921 gaacagaaca acctggcccc cagtcgtctt gaacagccac tttcctaggc tgctcactcg
     1981 ctgtctgcct tctcaggctt tggggcctta gccaccttct tcaccgtggc ctcaaaggcg
     2041 ccctcgtcct cgtcgcattc cagctcgcgg gcgagatcgc ggaatttgtc agcttgggtt
     2101 gtgtcgtgct ttggttcggt catggccctt cttcctaaac ctgagatcgt ggcgttcatc
     2161 gacgaatctg gtgacttcga actcgcaaag atagacccaa attaccccgt ctgcgcgcaa
     2221 tgcgcattga cctgcacggt tgacgaatat ctggcgaagg ccgtgccaaa cctcatgaat
     2281 ttgaaatacc acttcttcgg caatgaggct gtggtcatgc acggccacaa gatcagaaag
     2341 cgaagcctgc cgttcgacat cctgaaggat gacgaaatcc gggccgagtt tatggacgcg
     2401 gtgagttggg ccattgggca gctggagggt tgcctaatcg tggcggcggt tcacaagccc
     2461 cggcacgtcg gtcaatacat tcacccgacc gatccctttt tcctcagcct gcaattcctg
     2521 cttgagcggc tgtatatgca ctgggtcggc aagctggttg gggggcgccg gctgctttgc
     2581 gtgtttgaga agcgaggccc caaggaagac gagcggacca gaaactggtt tcatcaaatt
     2641 tgtgacggcg acaactaccg agcgcagcgc ttccccttcg acatcgattt tcggtccaag
     2701 gacgacaacg tcattggtca ccagtacgcc gacctcgtgg cctacgccgc ctgccgattt
     2761 gttgagactg gcgatggcga acgaagggat tggatggcag tgaagggcaa gctgagaacc
     2821 gttgacgggc ggtatgaaac ccacggcctc aaggtgttcc cctaatagca gaagggccgc
     2881 agtttcctgc gacccagcgc cgaccgccta cggcgaccca tttcgcctgc taggctatcg
     2941 aaatcgacga aaaagtcaag agaatcaatc tgctgctaac cctttgatgg agcgccgctt
     3001 tcttcgtcgc ttcgtcctcc aacgctggtt gcgcggcttc cgaagctttt taggcttggg
     3061 tccggctgtc agttgaccgg tatgtaaggc gcttgccgac cacgccttgc aacgcccgtt
     3121 cagcgcgctt ggtatcgtcc acgccaaggg cgatccggtt ggaataccgg aagtcgaact
     3181 ccgccgcgta gcggtgcagg tgcttcttgg aggcgtgttg gtagacgccc cgcatgccgc
     3241 gtttgaagat gctgtagaag ccctcgacgg tgttcgtgtg aacggtgcga tccgcctgcg
     3301 agacgtattc cttgcgggcg tgccggacgg acttgtgatc ggcgaacgcc ttgccgggcg
     3361 tgatgtagga agtcgcctcg tcagtgatca ggcgagcttc gcgagcgagg ttcgcgttca
     3421 ggatttccgt caccgtagcg gcgttcaggt gatccaccac gagcgacctt gcgcggcccg
     3481 tctgacggtc aatgagcgtc atcaccatgt tcatgttgcg gatcgtggtg cgcgacttgg
     3541 gcttgtcagg gtcgataccg agataggttt catcgacttc gaccgaaccg ccgccctgac
     3601 cgaacggggc cagctcgcca gagcgcatcg cttcacggat tcgatgggcc aggaaccacg
     3661 cagttttgta ggtggtttcc agagtgcgtt cgagctgctt ggcgctgata cccttcttcg
     3721 acgagcagag caggtagatc gcctgcaacc acttcgtcat gggcaggtgg ctttcctcga
     3781 acagcgtgcc gacgcgaaca gtgaactgct tacggcagtc gccgcacttc ttcaggccgt
     3841 ggcggatcgc gccttccggg ttcgccttgc tcttggagcc gcgcacgttg gccagcgcat
     3901 agacgcggcc cgtgcagccg cagtgcgggc aaaccggctc ggagccccaa aggatgccct
     3961 ccacaaaggc gaaggcggcg gcttcatcgt gaaagtaggg cttggacagg acggacatgg
     4021 ggtcaaccgg ttggaatcgt tcgttgattc aacctacctc attccgtggg tttgtcaaag
     4081 acataatcgc caggacgcca tacaaaaagt tttgtgggct tgtctgccgg cgctatttcc
     4141 agggccggaa gtgcttcgac agcttcagcc cctggcgcag atagtgcgag ccatcctcgc
     4201 cgtagagccg ggtcgggcgg gcggtcagcg gctcgtagac cagccgggcc acggtctggc
     4261 catcctcgag caggaacggc gtttcgtggc tgcgcacctc caggacgccc ttggagccgc
     4321 gcgagtgggt ctcgtccgtg ccgaagcccg ggtcgaagaa gccggcgtag tgaacacgga
     4381 actcgcccac cgccggatcg atcggggtca tctcggccgc ctcgagcacc gggatctcga
     4441 tgtcgtcctt ggaggccagg atgtagaact cgttggggtc gagcagcagc tcgcccttcg
     4501 gcgactccag catctcccag aagtcgcgcg ggtcgtggcc gtcttcccgg tccaggtcga
     4561 tgaccccggc gtggcgtcgg ccgcggaagc cgacggtgcc gatcgacagg tcgacgccga
     4621 cgtcgcggct ggacaggcgc accggcgtgc cgcgcttgag ccgcagctgg ttcagccggg
     4681 tgccggcgcg gaccagcacc gagaaggtct gcggcgcgat ctcgatgtac agcggaccgg
     4741 tgtagccctc ggccacgtcg tcgaaggccg ggccgtggtc ggtcagcagc cgcacgaaca
     4801 catcgacccg gccgctcgag ctcttgggat tgccgcgcgc ggatacgccc ggcggcaggg
     4861 ccagccgctc ctgcacctcg gcgatgtaga cgcagccgcg ctcgagcacg gccccgcggg
     4921 tcaggtcaag ctcgtgcatg gccacgtcgg gcaggcggtc caagacccgg cgtcgcccgg
     4981 gcaggaagga ggcccgcacg cgccagcagc gctcgcccag cctcaggtcg agactggccg
     5041 gctggacctg gtcggagtcg aacggggtga gcgatccgat ggcctcccgg gcgatcagat
     5101 cctcgatgga ttggcaggga agaatcccgg cggcgtgagc gtcattcatc gtcgggccat
     5161 tactcatggc ccggggcgac aaacgcaagg ccccgccggc gccgaccggc ggttttagtg
     5221 gtgcgccttg ccgcccgccg gcgagagcac atagatgccg gcccccaccc cgaacagcat
     5281 cagcgcctcg gtgagggtgc cgacggcgtg cagctcgacc ggcgaatacc gggccagggc
     5341 ctcgcccagc accagcagga ccacattgat cgcgccgagc acgccgagaa tggcggcgaa
     5401 tacgcggaac accattgcaa gcatcgaact tctcccccgt ctgtgcggtt gggccttaac
     5461 cttaacctga aaagcctgga ctgtgtcgag gttccgacga acgccgttcg cgcatgacgt
     5521 cggggctatt ggtcgaggcg ctgcgcctcc tctgccgccg gtatcgggga cacgtacctt
     5581 cggtacatgt cccctcgccc ttgaccttcc ccggcccttt cctgttgatg ccgcaacccc
     5641 tgacgagaaa gccgtagcca tggccgaaga cccgcacgac tggcagcccg cgacccgcct
     5701 gatccgcggc ggcgtcgccc gcagcaacta cggcgaaatc agcgaagccc tctatctgac
     5761 ccagagcttc gcctatgaaa gcgccggggc ggccgaccgg cgcttttccg gcgacgatcc
     5821 cggcttcgtc tattcgcgct acgccaaccc gacgacgcag atgttcgagg aacgcctggc
     5881 gctgctcgag ggggccgagt actgccgcgc cctggcctcg ggcatggcgg cggtgcacac
     5941 cgccctgacc ggcctgctgc gcgccggcga ccatctggtg gccggccggg cgctgttcgg
     6001 ctcgtgcaac tggatcgtcc agacctggct gccgcggttc ggcgtcgaga ccaccctggt
     6061 cgacgcgacg gacctggggg cctgggaggc ggccatgcgg ccgaacacga aggtcgtgct
     6121 gctcgagagc ccggccaacc cgctgctgga gatcaccgac atcgccgccg tggcgaagat
     6181 cgcccacgcc gccggggcca gggtcgtggt cgacaatgtg ttcgccacgc cgatcttcca
     6241 gaagccgctc gcgctgggcg ccgatgtggt ggtctattcg gcgaccaagc acatcgacgg
     6301 ccagggccgg gttctgggcg gggcgatcct cgggggcaag gccctgctcg aggaggccta
     6361 tcgcgacatc atccgccaca ccggcccggc gatctcgccg ttcaacgcct ggatcctgct
     6421 caaggggctg gagacgctcg acctgcgggt gcgccgccag accgacagcg ccgcggccct
     6481 ggccgaccag atcggcgcca gtccgaaggt ccgccgcgtg ctctatccgg gccgcgccga
     6541 ccatccgcag gcggccgtcg cccgggcgca gatgagcggc ttttccaacc tgctggcctt
     6601 cgacctggga tcgcgcgagg ccgcctggcg tttcctcgac gcgctggaga tcttcgacat
     6661 ctccaacaac cttggagacg ccaagtcgat ggccacccac ccgtccacca ccacccatcg
     6721 cgccatgcca gaggcgaatc gtctggagat cggcctgacc gagggctacg tgcgcatgtc
     6781 ggtgggcctg gaggatgtca gggacctggc ccgcgacgtc caaagggcgc tcgacgccgc
     6841 ctgagcatac gcctgccgag tccggggcca tgagtcgcat caaccgccgg gggaccccgg
     6901 acagtccggt ctacgaggcc cgcacggggg acatcctggt gcgggtggcg ccggtctatc
     6961 tgcccgagga atccaacccc gccggcggcc gctggatctg gggctacacc gtcgagatcg
     7021 agaaccacgg ccgcgacacc gtcacccttg tctcgcgaca ctggatcatc accgacggcc
     7081 gcaaccggat cgaggaggtg cgcggcgccg gggtggtcgg cgaacagccc gtgctcggcc
     7141 cccgcgaggc cttccgctac cagtccggct gcccgctggg gacgccgtcg ggcgccatgc
     7201 gcgggaccta ccagatggtc accgcggccg gcgaggcctt cgaggtgacg atcccggagt
     7261 tctcgctgca tctgccgggc gcgcggcggc ggctgaacta ggccctgctc accgcgcccg
     7321 cttgaccgga aggatggccg cggtcgcgac cagcaccgtc acaaccccga cgacccagat
     7381 gagggtgtag tcgcccgtcg ccgcgacgat tccgcccgcc accgccggcg cgatggcctg
     7441 ccccagattg atggccagga tgccgatgcc gaggtcgcgg cctgccgccg cctggtccgg
     7501 caggacgtcg atcatcagcg cctggtccac gaccatgaag cagccgagcg ccagcccgat
     7561 cagcaccgcc tgcacgaaca gcgccggcag ggtcggcgag accagcggca cggccagggc
     7621 gaccgcgaac agcagggagc tggcgatgac gaagggcttg cggcggccga tccggtcgga
     7681 ccagcggcca cacaccgcca tggccaccat cgtcccgggc agggcggcga gtcccagcag
     7741 gggcgtgatc ctggcggcct cgagggcgcc cagcgccggc tggatgtagc tctgcagcat
     7801 atagagggtg aaggtcgtcg ccgccgaata ggcgaacatg atcaatacct tggacaccca
     7861 gaccaggccg aagtcgcggt cgagcagggc cacggtgaag gcccgcagct ggccgagggc
     7921 gtcgctcggc ggcgcccgca gatcgcggga gggccggtcg ggcgccccgg cgacgaaggc
     7981 ggccgcgaac aggagcaccc ccgtgaaggc gagataggcc cagaccccga cctcgccgaa
     8041 cagggcgccg ccgaggacgc ccccgccg
//
LOCUS       DAORNX010000080         8051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6605_length_8051_cov_7.511756, whole genome shotgun sequence.
ACCESSION   DAORNX010000080 DAORNX010000000
VERSION     DAORNX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6605_length_8051_cov_7.511756"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLO65_05195"
     CDS             <1..257
                     /locus_tag="PLO65_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27677.1"
                     /translation="DAVRQNLAEYGQIMVIAARREVVHATRVLPLREADVSRPCPNGG
                     RQTRVCHGRRGGSRFEDGVRDEASPMAAPLHGRMPVKAGR"
     gene            complement(1001..1774)
                     /locus_tag="PLO65_05200"
     CDS             complement(1001..1774)
                     /locus_tag="PLO65_05200"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRD27678.1"
                     /translation="MTRKIAFDAIENFRDFGDYPVGDRRLKRGVLYRAAHQSEATEAD
                     LETMRALGLSVIVDLRRPNERERSPSRRWQGFDGLVIENDMGHEEEDPWHVFIRSSDL
                     SARAFTDYMVGYYREAPFVDRHVDLYSRYFHALSETDGAVLIHCAAGKDRTGILAALT
                     HHLAGVHEDDILADFLLTNDPHRFERRAPQFAAMLEELTGRRPTQDAIMTALGVEAHY
                     LETAFAAIHARHGSLDAYLEDALGVDAARREAIAHRILD"
     gene            1921..2799
                     /locus_tag="PLO65_05205"
     CDS             1921..2799
                     /locus_tag="PLO65_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD27679.1"
                     /translation="MLIIENLTHVYPNGTVALDEVNLTVEKGMFGLLGPNGAGKSTLM
                     RTVATLQSPTSGHVRFGGIDVLKDPEQLRRTLGYLPQDFGVYPRVSALDMLDHMAVLK
                     GISNAKDRRETVETLLHQTNLWSVRKKAIAGFSGGMRQRFGIAQALIGDPSLIIVDEP
                     TAGLDPEERNRFLNLLAEIGENVVVILSTHIVEDVSDLCPAMAIICDGRIVGQGAPSD
                     LVNGLKGRIWMKVIDKAELADHQARYRVISTRLFAGRTVIHIISDADPGDGFKPVQGD
                     LEDVYFSTLSTHRKAA"
     gene            2810..>3933
                     /locus_tag="PLO65_05210"
     CDS             2810..>3933
                     /locus_tag="PLO65_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRD27680.1"
                     /translation="MFSKIAGFEFRYQLRNPVFWVAAGIFFLLTFGAVTIEQIRIGGG
                     GGGIHKNAPFMIATMHLILSIFYMFVSTAFVANIIVRDDETGFGAILRSTRISRFDYL
                     YGRFTGAFAAVCLSFLAVPAAIVVGSFMPWVDRETLGPLTLEPFVFSYFALALPALLL
                     TSSMFFTLATVTRSLMWTYIGVIALLILWTVATIALDKPEYEKIAAFWEPFGAAAFGL
                     ATKYWTVADRNALTPALTGALLFNRVLAVGLSALFLTLAYALFRFQTGARSGKRRARA
                     QLATTAAPDAPPPLLKALPKARFDGSAAWAQLLARTRMDMGQVFGSPAYLVLLAMGLF
                     NSIGALWFTTDETGYGGQIWPVTRALIPPLVGSFSLIPMI"
     assembly_gap    3934..3973
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3974..6411
                     /locus_tag="PLO65_05215"
     CDS             <3974..6411
                     /locus_tag="PLO65_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRD27681.1"
                     /translation="ERERKTHEIVDSTPVPDWAFVGPKTLAISLVLLSTLLVSVAAAV
                     IIQALKGYTHFQLDAWALWYVLPQAVDWVLLAVLAVFLQTLSPHKFVGWGLMVIYLIT
                     TITFTNLGFEHNLYNYGGAPGVPLSDMNGQGKFWIGAWWFRLYWSAFAVLLVVLSYAL
                     WRRGTESRLWPRLRRLPRRLTGKAGLTFATGLAIFAASGLFIYVNTNVWNTYRTSIDD
                     EQWQADFEKTLLRYEHLPRPKIVAMKLDVDLRPHAPSATTWGSYVIENRTGAPLKEIH
                     VLFDRDLKVKALSIQGARPKKTFERFNYRIFALDTPMLPGDRRMVTFTTERSQRGFRN
                     RGNETRVVDNGTFINNSEIAPLLGMERQGLLRDRAKRRKYGLPPELRMPPLGDPASMQ
                     FNYLRHDADWVTSDITVTTDADQTPVAPGYRVSETVRDGRRTARFVTEAPILPFLSVQ
                     SARYTVSKETYKGIDLAVYYDAQHPWNIDRMKAGMKASLDYFQANFSPYQFRQVRFLE
                     FPAYADFAQSFANTIPWSENLFFIADYRDKSKIDMVTYIGAHEIGHQWWAHQIIGADQ
                     QGSTVLSETLAQYSALMVMKHMYGEDQIRKFLKFELDRYLRARGSELVEELPLYKVED
                     QGYIHYRKGSLVMYRLQREIGEAAVNRALRSLLAKFAFKGAPYPTSRDLVAALRAEAP
                     ADKQGLITDLFEKITLYDLKTRAMTVKKRADGRFDVTLTVEAKKLYADGKGRETPAPM
                     AETLDVGLFTAMPGDKGFDSSKVVTLVTRPIRTGTQTLTFTVDKAPKYGGVDPYNYLI
                     DRNSDDNVLKAGG"
     gene            complement(6440..7012)
                     /locus_tag="PLO65_05220"
     CDS             complement(6440..7012)
                     /locus_tag="PLO65_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HRD27682.1"
                     /translation="MNRTLLLAGAAAMALTLAACNKKPDEAAMGAPDANPAATIPTPS
                     DEAAAPDFVNKAAASDTFEVESSRIALDRSSNSEVKAFASMMVEAHTKSTEDLKAAIA
                     GSGLALTPPTTLPDDKASALQDLRSASAGDFDKKYMDAQVDGHQGALDLMTRYAQDGD
                     NPSLKAAAATMAPVIQQHLDKARALRDALK"
     gene            7093..>8051
                     /locus_tag="PLO65_05225"
     CDS             7093..>8051
                     /locus_tag="PLO65_05225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD27683.1"
                     /translation="MSSDKKPQAPGPLDGLTVIEMGTLIAGPFCGQILGDFGAEVIKI
                     EDPKAGDPMRQWGRSLPKGHSPWWPVIGRNKKSVTLNLRTGEGQAIARRLIGGADVLV
                     ENFRPGALEKWGLGYDSLSADNPGLVMARVSGFGQTGPYASRAGYALIGEAMGGLRYI
                     TGEPDRAPARAGISVGDSLAGLHAALGTMMALHARHRSGKGQVVDAAIYESVLSVMEN
                     LITEYGLTGYIRERSGSVLPGIAPSNAYPTRDGALVVIGANQDTLFKRLCEAMGRPEL
                     AQDPRYAGHAARGENQAALDALIGAWTATLDADALLALMESSGV"
BASE COUNT         1234 a         2742 c         2751 g         1284 t
ORIGIN      
        1 tcgacgccgt ccggcagaac ctcgccgagt acggccagat catggtcatc gccgcccggc
       61 gcgaggtcgt tcatgccact cgagtcctgc ccttgagaga ggccgacgtc tcgcggcctt
      121 gccccaacgg tggacgtcaa acgcgcgttt gtcacgggcg ccgtggcggc agccggttcg
      181 aggatggagt ccgcgatgag gcgtcgccca tggcggcgcc gcttcacggg cggatgcccg
      241 tgaaggcggg gaggtgacac ggcgcgctcc ggtctcaccc ggatgctgga ttgtgagcgt
      301 gtgagaaggg aaagtccgtc tcgcgccgcg aggtccgttt tccgggggcc gccggtgggc
      361 agcgccttta acgcttagcc ggcagaagcc tccggcgggc gggcggcggc gaagccgtcc
      421 ccggaccgtg agcgtaaccc ccgctcacct gtccccgctc gaaccccgtc ccgccgccgc
      481 ctcggatcgc ctggcccaga cgccctcccc gtgcagcggg acaggcagca gtatgggggg
      541 ggtggggagg ggcgagggga agattgtccg gcgcggcggg gctacgcggg cggagttgag
      601 ggatttcaag gggttggacg cggggatccg aaaccatccc ccggaccttc ctctcccgga
      661 gggagagggg gaccagccgg ggctggtgga gagggcggcg ccggtttcga gggggcaggg
      721 cgctcgcctc gccaacccgc ggcgcatccc tctccgaccc ggcttcgccg ggccacctct
      781 ccccgcgggg gagggcggat ggtgcgcgaa cgaccccagg gccaatcgac attcacgacc
      841 tggggccgct cttttaccga ttgtccccgc gaaggcgggg acccaggccc ggatggcggc
      901 cgaagcggcg cttcaggatc cgaaacatcg cttgggttcc cgccttcgcg ggaacgatcg
      961 gagcctttgg cagatcccgc gcgctggatg tcgattggtc ctagtcgagg atcctgtggg
     1021 cgatggcttc gcggcgggcg gcgtcgacgc cgagggcgtc ctcgaggtag gcgtcgagcg
     1081 agccgtgccg ggcgtggatc gcggcgaagg ccgtctccag atagtgggcc tcgacgccca
     1141 gggcggtcat gatcgcgtcc tgcgtcgggc ggcggccggt caactcctcg agcatggcgg
     1201 cgaactgcgg cgcgcggcgc tcgaaccggt gcggatcgtt ggtcagcagg aagtcggcga
     1261 ggatgtcgtc ctcatgcacc ccggccaggt ggtgagtcag ggccgccagg atgcccgtgc
     1321 ggtccttgcc cgccgcgcag tggatcagaa cggcgccgtc cgtttccgac agggcgtgga
     1381 agtagcggct gtagaggtcg acgtggcggt cgacgaacgg ggcctcgcga tagtagccga
     1441 ccatgtagtc ggtgaaggcc cgggcgctca ggtcggagct gcggatgaac acatgccagg
     1501 gatcctcctc ctcgtggccc atgtcgttct cgatcaccag gccgtcgaag ccctgccagc
     1561 ggcgactggg gctgcgttcg cgctcgttcg gccggcgcag gtcgacgatg accgacaggc
     1621 ccagggcccg catggtctcc aggtcggcct cggtggcctc ggactggtgg gcggcgcggt
     1681 acagcacgcc gcgcttcagg cggcgatcgc cgacggggta gtcgccgaaa tcacggaagt
     1741 tctcgatggc gtcgaaggcg atcttgcggg tcatgcacgg ttcctagcga aggtcggtga
     1801 cgatggcgag acgttgtgcg ccttaccgtt ctttcacttg ctccccgccg ccgcgcccgc
     1861 catgactcca ggcgggggag tgctgagcgt aaatttcaac accggggggt gggcttgagc
     1921 atgctgatta tcgagaacct gacgcacgtc tatccgaacg gaacggtggc gctcgacgag
     1981 gtcaacctga cggtcgagaa gggcatgttc ggcctgctcg gaccgaacgg ggccggcaag
     2041 tcgaccctga tgcgcacggt cgccaccctg cagtcgccga cgtccggcca cgtccggttc
     2101 ggcggcatcg acgtactcaa ggaccccgaa cagctgcgcc gcacgctcgg ctacctgccg
     2161 caggacttcg gcgtctatcc gcgggtctcg gccctcgaca tgctcgacca catggccgtg
     2221 ctcaagggca tctccaacgc gaaggatcgc cgggagacgg tcgagaccct gctccaccag
     2281 accaacctgt ggagcgtgcg caagaaggcc atcgccggct tctccggcgg catgcgccag
     2341 cgcttcggca tcgcccaggc cctgatcggc gacccgtcgc tgatcatcgt cgacgagccg
     2401 accgccggcc tcgaccccga ggagcgcaac cgcttcctca acctgctggc cgagatcggc
     2461 gagaacgtcg tggtcatcct ctcgacccac atcgtcgagg acgtgtccga cctgtgcccg
     2521 gccatggcga tcatctgcga cggccgcatc gtcggccaag gcgcgcccag cgacctggtc
     2581 aacggcctga agggtcgcat ctggatgaag gtcatcgaca aggccgagct ggccgaccac
     2641 caggcccgct accgcgtcat ctcgacccgg ctgttcgccg gccgcacggt catccacatc
     2701 atttccgacg ccgatccggg cgatggcttc aagccggtgc agggggacct ggaggacgtc
     2761 tacttctcca ccctctcgac gcaccgcaaa gccgcctgag ggccccggca tgttcagcaa
     2821 gatcgcgggg ttcgagttcc gctaccagct gcgcaacccg gtgttctggg tcgccgccgg
     2881 catcttcttc ctgctgacct tcggcgcggt caccatcgag cagatccgca tcggcggcgg
     2941 cggtggcggc atccacaaga acgcgccgtt catgatcgcg acgatgcatc tcatcctctc
     3001 gatcttctac atgttcgtct ccacggcctt cgtggccaac atcatcgtgc gggatgacga
     3061 gaccgggttc ggcgcgatcc tgcgctcgac gcggatttcc cggttcgatt acctctacgg
     3121 ccggttcacc ggcgccttcg cggccgtctg cctgtcattc ctggccgtgc cggcggcgat
     3181 cgtggtcggc agcttcatgc cctgggtgga ccgcgagacg ctcggcccgc tgacgctcga
     3241 accgttcgtc ttctcctact tcgccctcgc cctgcccgcg ctgctgctga cgtcctcgat
     3301 gttcttcacc ctggcgacgg tgacccgctc gctgatgtgg acctatatcg gcgtcatcgc
     3361 cctgctgatc ctttggaccg tggcgacgat cgccctggac aagcccgagt acgagaagat
     3421 cgccgccttc tgggagccgt tcggggccgc cgccttcggc ctggcgacca agtactggac
     3481 ggtcgccgac cgcaacgcgc tgaccccggc gctgaccggg gccctgctgt tcaaccgcgt
     3541 cctcgccgtg ggcctgtcag ccctgttcct gactctggcc tacgccctgt tccgcttcca
     3601 gacgggagcc cggtcgggca agcggcgcgc gcgcgcccag ctggcgacga ccgccgcccc
     3661 cgacgcgccg ccgccgttgc tgaaggcgct gcccaaggcg cggttcgacg gatcggcggc
     3721 ctgggcccag ctgctggccc gcacccgcat ggacatgggc caggtgttcg gcagtccggc
     3781 ctatctggtg ctgctggcga tgggcctgtt caactccatc ggcgccctgt ggttcactac
     3841 cgacgagacc ggctacggcg gccagatctg gccggtgacg cgcgcgctga tcccgccgct
     3901 ggtcggctcc ttcagcctca ttccgatgat catnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnngggaacg cgagcggaaa acccacgaga tcgtcgattc gacgcccgtg
     4021 cccgactggg ccttcgtcgg ccccaagacc ctggccatct cgctcgtgct gctgtcgacc
     4081 ctgctggtca gcgtcgccgc cgcggtgatc atccaggcgc tcaagggcta cacccacttc
     4141 cagctcgacg cctgggcgct gtggtacgtg ctgccgcagg ccgtcgactg ggtgctgctg
     4201 gccgtgctgg cggtgttcct gcagaccctt tcgccgcaca agttcgtcgg ctggggcctg
     4261 atggtgatct acctgattac caccatcacc ttcaccaacc tcggcttcga gcacaatctc
     4321 tacaactacg gcggggcgcc gggcgtgccg ctgtccgaca tgaacggcca gggcaagttc
     4381 tggatcggcg cctggtggtt ccgcctctac tggagcgcct tcgcggtcct gctggtcgtg
     4441 ctcagctacg ccctctggcg gcggggcacc gagagccggc tgtggccgcg cctgcggcgc
     4501 ctgccgcggc ggctgaccgg caaggccggg ctgacgttcg cgaccggcct ggcgatcttc
     4561 gccgcctcgg gcctgttcat ctacgtcaac accaacgtct ggaacaccta ccgcaccagc
     4621 atcgacgacg agcagtggca ggcggacttc gagaagaccc tgctgcgcta cgagcacctg
     4681 ccgcggccga agatcgtggc gatgaagctg gacgtcgacc tgcggccgca cgcgcccagc
     4741 gccacgacct ggggctcgta tgtgatcgag aaccgcaccg gcgcgccgtt gaaggagatt
     4801 cacgtcctgt tcgaccggga cctgaaggtg aaggccctgt cgatccaggg ggcccggccg
     4861 aagaagacct tcgagcggtt caactaccgg atcttcgcgc tcgacacccc gatgctgccc
     4921 ggcgaccggc ggatggtgac cttcaccacc gagcggtcgc aacgcgggtt ccgcaaccgg
     4981 ggcaacgaga cccgggtcgt cgacaacggc accttcatca acaacagcga gatcgcgccg
     5041 ctgctgggca tggagcgcca ggggctgctg cgggaccgcg ccaagcgccg caagtacggc
     5101 ctgccgccgg agctgcgcat gccgccgctg ggcgaccccg cctcgatgca gttcaactac
     5161 ctgcgtcacg acgccgactg ggtgacctcg gacatcaccg tcacgacgga cgccgaccag
     5221 actccggtcg cgccgggata ccgcgtctcg gaaacggtcc gagacggccg acgcacggcc
     5281 cgcttcgtca ccgaagcgcc gatcctgccg ttcctgtcgg tgcagtcggc gcgctacacg
     5341 gtcagcaagg agacctacaa gggcatcgat ctggccgtct attacgacgc ccagcatccg
     5401 tggaacatcg accggatgaa ggccgggatg aaggcgtcgc tggactattt ccaggccaac
     5461 ttcagcccct accagttccg ccaggtgcgg ttcctcgaat tcccggccta cgccgacttc
     5521 gcccagtcgt tcgccaacac catcccctgg tcggagaacc tgttcttcat cgccgactac
     5581 cgcgacaagt cgaagatcga catggtcacc tacatcgggg cccacgagat cggccaccag
     5641 tggtgggcgc accagatcat cggcgccgac cagcagggct cgaccgtgct gtcggagacg
     5701 ctggcgcaat acagcgccct gatggtcatg aagcacatgt acggcgagga ccagatccgc
     5761 aagttcctca agttcgagct cgatcgttac ctgcgcgcgc ggggcagcga gctggtcgag
     5821 gaactgccgc tctacaaggt cgaggaccag ggctacatcc actaccgcaa gggctcgctg
     5881 gtgatgtacc ggctgcagcg ggagatcggc gaggcggcgg tcaaccgggc cctgcggtcc
     5941 ctgctggcga agttcgcctt caagggcgcg ccctatccga cgtcgaggga cctggtcgcg
     6001 gccctgcgcg ccgaggcccc ggccgacaag cagggcctga tcaccgacct gttcgagaag
     6061 atcaccctct atgacctgaa gaccagggcc atgacggtga agaagcgggc cgacggccgc
     6121 tttgacgtga ccctgacggt cgaggcgaag aagctctacg ccgacggcaa gggcagggaa
     6181 acgcccgcgc cgatggccga gacgctcgac gtcggcctgt tcacggccat gccgggcgac
     6241 aaggggttcg attccagcaa ggtcgtgacc ctggtgaccc ggccgatccg gaccggaacc
     6301 cagaccctca ccttcaccgt cgacaaggcg ccgaagtacg gcggggtcga tccctacaac
     6361 tacctgatcg accgcaactc ggatgacaac gtgctgaagg cgggcggcta gcggttcgcg
     6421 tcgcccgccc tccgccggcc tacttcaggg cgtcgcgcag cgcccgggcc ttgtccaggt
     6481 gctgctggat gaccggggcc atggtggcgg cggcggcctt gagcgacggg ttgtcgccgt
     6541 cctgggcgta gcgggtcatc aggtcgagcg cgccctggtg gccgtcgacc tgggcgtcca
     6601 tgtacttctt gtcgaagtcg ccggccgagg ccgagcgcag gtcctgcagg gccgaggcct
     6661 tgtcgtcggg cagggtggtc ggcggggtga gggccaggcc ggagccggcg atggccgcct
     6721 tgaggtcctc ggtgctcttg gtgtgggcct cgaccatcat cgaggcgaag gccttgacct
     6781 cgctgttgct cgaccgatcc agcgcgatcc tgctcgactc gacctcgaac gtgtcgctgg
     6841 cggccgcctt gttgacgaag tcgggggccg cggcttcatc cgagggggtc gggatggtcg
     6901 cggcggggtt ggcgtccggg gcgcccatgg ccgcctcgtc cggcttcttg ttgcaggcgg
     6961 ccagggtcag ggccatggcg gcggcgccgg ccaggagaag ggtgcggttc atgtcggaga
     7021 ctccactgga ttgacaagct ggcccgccgc ccccaagcgg gccgggtgat gacaaaacct
     7081 cacgggggcg tgatgagttc cgacaaaaag ccgcaagccc ccgggccgct cgacggcctg
     7141 accgtgatcg agatggggac gctgatcgcc gggccgttct gcggccagat cctcggcgac
     7201 ttcggcgccg aggtgatcaa gatcgaggac ccgaaggccg gcgacccgat gcgccagtgg
     7261 ggccgcagcc tgcccaaggg ccattcgccc tggtggccgg tgatcggccg caacaagaag
     7321 tcggtgacgc tcaacctgcg aaccggcgag gggcaggcca tcgcccgcag gctgatcggc
     7381 ggggccgacg tgctggtcga gaacttcagg cccggcgcgc tggagaagtg gggcctcggt
     7441 tacgacagcc tgtcggccga caatccgggc ctggtgatgg cccgggtatc gggcttcggc
     7501 cagaccgggc cctacgcgtc gcgcgccggc tacgccctga tcggcgaggc catgggcggg
     7561 ctgcgctaca tcaccggcga gcccgaccgc gcccccgccc gggccggcat ttcggtcggc
     7621 gacagcctgg cgggactgca tgcggcgctc ggaaccatga tggccctgca cgcccgccat
     7681 cgcagcggca agggccaggt cgtcgacgcg gcgatctacg aaagcgtgct gtcggtgatg
     7741 gagaacctga tcaccgaata cggcctgacc ggctacatcc gcgagcggtc gggctcggtg
     7801 ctgcccggca tcgcgccgtc gaacgcctat ccgacgcggg acggggcgct ggtggtcatc
     7861 ggggccaacc aggacaccct gttcaagcgc ctgtgcgagg ccatggggcg gccggagctg
     7921 gcccaggatc cgcgctacgc cggccacgcc gcccgcggcg agaaccaggc cgcgctcgac
     7981 gccctgatcg gcgcctggac ggcgaccctc gacgccgacg cgttgctggc cctgatggag
     8041 tccagcggcg t
//
LOCUS       DAORNX010000081         8049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6607_length_8049_cov_6.727421, whole genome shotgun sequence.
ACCESSION   DAORNX010000081 DAORNX010000000
VERSION     DAORNX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_8049_cov_6.727421"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PLO65_05230"
     CDS             <1..1075
                     /locus_tag="PLO65_05230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRD27684.1"
                     /translation="AAVERAGLDPAKVEQIIMGCVLPAGLGQAPARQAGLGAGLSLGV
                     EATTVNKMCGSGMQAAIMAHDALAAGSVDLVVAGGMESMTGAPYLMTKHRGGARIGHD
                     TISDSMYLDGLEDAYEPGRLMGSFAEDSARAYQFTREDQDAYAIESLNRARAATESGA
                     FSAEITPVTVRTRKGEEVVGQDEQPMKADPSKIPGLKPAFAKDGTITAANASSISDGA
                     AALVMTRESVAKALGLPIVARVVAHAAHAQEPGLFTTAPVPAMRKALKKAGWTAEDVD
                     LWEVNEAFAVVAMIAMRELGIDRARLNVNGGATALGHPIGASGARILATLLSALQARG
                     GKRGMASLCIGGGEGTAMAVELV"
     gene            1206..1478
                     /locus_tag="PLO65_05235"
     CDS             1206..1478
                     /locus_tag="PLO65_05235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27685.1"
                     /translation="MTHVTDNPAAGRFELIEQGQIAYADYRRSPGRLVIDYVFSPPAL
                     RGAGTAGRLMQGVVAAARAENLKITPLCGYAAAWLKRHPETADLLA"
     gene            1531..2010
                     /locus_tag="PLO65_05240"
     CDS             1531..2010
                     /locus_tag="PLO65_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27686.1"
                     /translation="MFRIMTMGCALALNLTLAGAAGAQTYLSDWSAGDMRAVLTAAGA
                     TVTADGLHDDGNPYLSAKTPGGLKFTVSGRVCSGAPAARRCKGALLESRFTLASDAEV
                     ETRIKQLDFAAVSITNGGEGDLLVSRYLILDYGVNRQNLEINVSVFTGIAEDIWSKL"
     gene            2028..2510
                     /locus_tag="PLO65_05245"
     CDS             2028..2510
                     /locus_tag="PLO65_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27687.1"
                     /translation="MLKPFVLAGAVALALTVPGSAFAQSGVLSDWGVSDMKQALTGAG
                     ATITKEGTLDDGAPYIGATSTNGMKFVVYGTVCSGAPKRCKGANMTASFTLDSDAAVD
                     AREKKIDRSAVGVRNAGDNSLEVSRYLIFDNGVTRGNVQTNIEVFIEVAEDIWNGGGE
                     "
     gene            2598..3875
                     /locus_tag="PLO65_05250"
     CDS             2598..3875
                     /locus_tag="PLO65_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD27688.1"
                     /translation="MTASGFNSLDRDLRRRAEAVIPGGMYGHQSVGLLPDDYPQFFRS
                     GDGAHITDADGRRYLDLMCAYGPNLFGYANPQVDAAYVRQLGQGDALTGPTRLMVELA
                     EAFVAMVTHADWAMFCKNGTDATTMAMMTARQHTGRKTLVLAKGAYHGAAPWCTPRPA
                     GTTAEDKANQIFCAYNDIASLEAAVAKAGDDLAAIFAAPFRHDAFIDQAAPEIAYARR
                     ARELADQTGALLIVDDVRAGLRLARDCSWSAIGVQPDLSTWGKCIANGHPISALLGND
                     KARKAAASIYVTGSFWYAGAAMAAGLETLRLVRETDYLERITALGETLRAGLAERAAA
                     AGFGFRQTGPGVMPLFLFDDDPDLRKGFCWSSAMLAKGVYVHPWHNMFLCAAMTDADI
                     QFALDAAEQAFQTLKTQARDLPPVEKLAFLAAR"
     gene            3895..5322
                     /locus_tag="PLO65_05255"
     CDS             3895..5322
                     /locus_tag="PLO65_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD27689.1"
                     /translation="MGFEAYADYDGLGLADLVNRGEVSPAELVEAAIERIERHNPTLN
                     AVVHKAYDEARKVAAGPLPDGPFKGVPFLIKDLGAKVVGWPRTSGSNYADVPADSEDS
                     VLVRRYRESGVVLLGKTNTPEYGIPGVTQSARLGPCGNPWNPEHISGGSSGGAASATA
                     SGMVPLAHASDGLGSIRIPAANCGLVGMKITRDRTPICEVTDGAMGFSVHHVVSRTVR
                     DSAAMLDATDGPQPGDPFTHPRKERPFLEEVSRSPGKLRIAWSNETPSGRPMGDEMIA
                     ATQKTVETLKALGHEVFEMGLGIDYRLMYRAQGLVSASNFAATMMRRAEKFGREPEPG
                     ELGPLAQRGWDGGRRVTGQQALWGWQTLRLINIQILERFQAFDVFLTPTMSYPSPRID
                     WLDPLTVDVKEFDKRQAATYGTTPPFNFTGQPSLSLPLWQSEANLPLGMMFTGRFGDE
                     ATLYRLAGQLEKEIPWIGRKPPVWG"
     gene            5424..6386
                     /locus_tag="PLO65_05260"
     CDS             5424..6386
                     /locus_tag="PLO65_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRD27690.1"
                     /translation="MKAAVYYENGSPDVLKYEDVPDPVCHPKGVIIRVLAVSIEGGDT
                     LNRWRGPLVTRPHIVGYQASGEIVEVGSEVTHLKVGQKVATVDGFGSHAELRPVPARN
                     CWPIPDSLDPRHAAVIPVAFGTAHDCLFEFGRLKAGETVLVQAGASGVGLAAIQLAKR
                     AGATVLATASSDARLEKLKAYGLDHGINYTKDAVPEAVMKITDKKGVNLVVDSVGGPT
                     LQGSILSLGYRGRVSMVGQAGREPMVVDVGSLMGKNGSLSGVFLGAEIMTDRAHDMIQ
                     GLIEDAAKGEIKVVIDREFPLSEAAAAHAYIESRQAVGRVLLIP"
     gene            6601..7602
                     /locus_tag="PLO65_05265"
     CDS             6601..7602
                     /locus_tag="PLO65_05265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD27691.1"
                     /translation="MTPLSVLDLAPVVQGSTVQQALHNSRDLAQHAERWGYNRFWLAE
                     HHNMRGIASAATSVLIGHVAEGTSTIRVGSGGIMLPNHAPLQIAEQFGTLAALYGPRI
                     DLGLGRAPGTDQITARALRRTMVGDVDRFPADVVELMRYFQPAQPGQAVVATPGEGLE
                     VPIWMLGSSQFGAHLAAALGLPYAFASHFAPQEMETAVAAYRARFEPSAQRDKPYVML
                     ALNVFAADTDEEARLLFTSLQQAFVNLATGMPGRLPPPMEGYEARLPADAKARLASAL
                     RCSVVGSRETVRKGLAEFIERTGADELILTGQIHDHAARLRSFEIAAEVARELADAA"
     gene            complement(7696..>8049)
                     /locus_tag="PLO65_05270"
     CDS             complement(7696..>8049)
                     /locus_tag="PLO65_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD27692.1"
                     /translation="VAMVLFGPQIAGLYLGHGRAGDGAVVVLATQFLLVAAAFQVFDA
                     LQVVGAQALRGLKDARMPMVLAGVSYWLAGAPVCILLGVGLKMQGLGIWIGLAFGLAV
                     AAAAMTWRFWRITRV"
BASE COUNT         1280 a         2920 c         2704 g         1145 t
ORIGIN      
        1 cgccgccgtc gaacgggccg ggctcgatcc ggcgaaggtc gagcagatca tcatgggctg
       61 tgtcctgccg gccggccttg gccaggcccc cgcccgccag gccggcctcg gcgccgggct
      121 gtcgctcggg gtcgaggcga ccacggtcaa caagatgtgc ggcagcggca tgcaggcggc
      181 gatcatggcc catgacgccc tggcggccgg ctcggtcgac ctggttgtcg ccggcggcat
      241 ggagagcatg accggcgcgc cctatctgat gaccaagcac cgcggcggcg cccgcatcgg
      301 ccacgatacg atcagcgaca gtatgtatct cgacggtctg gaggacgcct atgagcccgg
      361 ccggctgatg ggctcgttcg cggaggacag cgcccgcgcc taccagttca cccgcgaaga
      421 ccaggacgcc tacgccatcg aaagcctgaa ccgggcccgg gccgcgaccg agtcgggggc
      481 cttctcggcc gagatcacgc cggtgacggt caggacccgc aagggcgagg aggtcgtcgg
      541 ccaggacgag caaccgatga aggccgaccc gtcgaagatc ccgggcctca agccggcctt
      601 cgcgaaggac ggcaccatca cggccgccaa cgccagctcg atcagcgacg gcgccgcggc
      661 cctggtgatg acccgcgaga gcgtggcgaa ggcgctcggc ctgccgatcg tcgcccgcgt
      721 cgtggcccac gcggcccatg cccaagagcc gggcctgttc accacggcgc cggttccggc
      781 gatgcgcaag gcgctgaaga aggccggctg gacggccgag gacgtcgacc tgtgggaggt
      841 caacgaggcc ttcgccgtgg tggccatgat cgccatgcgt gagctgggca tcgaccgggc
      901 ccggctcaac gtcaacggcg gcgccacggc gctggggcac ccgatcggcg cgtccggcgc
      961 gcgcatcctg gccaccctgc tctcggcgct gcaggcccgc ggcggcaagc gcggcatggc
     1021 cagcctgtgc atcggcggcg gcgagggcac ggcgatggcg gtggaactgg tttagggctt
     1081 ggggaacagg gagcggggag cagttccggc ttgcacgccg gccgttccgc gcgtcccttc
     1141 tgctactccc tactccctac tccctactcc ctactcccca tggtccactc caagcccagt
     1201 cccccatgac ccacgtaacc gacaaccccg ccgccggccg gtttgaactg atcgagcagg
     1261 ggcagatcgc ctacgccgac taccgtcgct cgcccggccg gctggtgatc gactatgtct
     1321 tctcgccccc ggccctgcgc ggcgccggca cggccgggcg actgatgcag ggcgtggtgg
     1381 ccgcggcccg cgccgagaac ctcaagatta cccccctttg cggttatgcg gccgcctggc
     1441 tcaagcgcca tcctgaaaca gcggacctgt tggcgtagca ttggtcctcg tcctagactg
     1501 ccgccgggga cgaagagcgg gatagggttg atgttcagga tcatgaccat gggctgcgcc
     1561 ctggcgctga acctgacgct ggccggcgcg gccggtgcgc agacctatct ctcggactgg
     1621 agcgcgggcg acatgcgcgc ggtgctcacc gccgccggcg ccacggtcac cgccgatggc
     1681 ctgcatgacg acggcaaccc ctatctctcg gcgaagacgc ccggcggcct gaaattcacc
     1741 gtcagcggtc gggtctgcag cggcgcgccg gccgcccggc gctgcaaggg cgccctgctg
     1801 gagtcccgtt tcaccctcgc ctcggacgcc gaggtcgaga cccggatcaa gcagctcgac
     1861 ttcgcggctg tctcgatcac caacggcggc gagggcgacc tgctggtctc ccgctacctg
     1921 atcctggact acggcgtgaa ccgccagaat ctggagatca acgtcagcgt cttcaccggg
     1981 atcgccgagg acatctggag caaactctag tgaacaggaa aaatcacatg ctgaaaccat
     2041 tcgtcctggc gggcgccgtg gccctcgccc tcacggtccc tgggagcgcc ttcgcccaga
     2101 gcggcgtgct atctgactgg ggcgtgtccg acatgaagca ggccctcacc ggcgccggcg
     2161 cgacgatcac caaggaaggg acgctggacg acggcgcgcc ctacatcggc gcgacctcga
     2221 ccaacggcat gaagttcgtg gtctacggca ccgtctgcag cggcgcgccc aagcgctgca
     2281 agggcgccaa catgaccgcc agcttcacgc tggactccga cgccgctgtc gatgcccggg
     2341 agaagaagat cgaccggtcg gcggtcggcg tgcgcaacgc cggcgacaac tcgctggaag
     2401 tctcgcgcta cctgatcttc gacaacggag tcacgcgcgg caacgtgcag accaacatcg
     2461 aggtgttcat cgaggtggcc gaggacatct ggaacggcgg cggcgaatag acagcctgtg
     2521 atcgcgatcc ggccgcgggg gcgactccgc ggccgtttca ttgctaacgt cctccctgga
     2581 cagggcggag acgacggatg acggccagcg gtttcaacag cctcgaccgc gatctgcgcc
     2641 ggcgggccga ggcggtgatt cccggcggca tgtacggcca ccagtcggtc ggcctgctgc
     2701 cggacgacta tccgcagttc ttccgctcgg gcgacggcgc ccacatcacc gacgcggacg
     2761 gccggcgcta cctcgacctg atgtgcgcct atggccccaa cctgttcggt tacgccaatc
     2821 cccaggtcga cgcggcctat gtgcgccagc tggggcaggg cgacgccctg accggtccaa
     2881 cccgcctgat ggtcgagctg gccgaggcct tcgtggccat ggtcacccac gccgactggg
     2941 cgatgttctg caagaacggc acggacgcca cgaccatggc gatgatgacc gcgcgccagc
     3001 acaccggccg caagacgctg gtgctcgcga agggcgccta tcacggggcc gcgccctggt
     3061 gcacgccgcg cccggccggc acgactgcag aggacaaggc caaccagatc ttctgcgcct
     3121 acaatgacat cgccagcctg gaggcggccg tcgcgaaggc tggcgacgac ctggccgcca
     3181 tcttcgccgc gccgttccgc catgacgcct tcatcgacca ggccgcgccg gagatcgcct
     3241 acgcccgccg cgcccgcgag ctggcggacc agaccggcgc cctgctgatc gtcgacgatg
     3301 tccgcgccgg cctgcgcctg gcccgcgact gcagctggtc ggcgatcggc gtgcagccgg
     3361 acctgtcgac ctggggcaaa tgcatcgcca acggccatcc gatctcggcc ctgctgggca
     3421 atgacaaggc gcgcaaggcg gccgcctcga tctatgtcac cggctcattc tggtatgccg
     3481 gcgcggccat ggcggcgggt ctggagaccc tgcggctggt gcgcgagacc gactatctgg
     3541 agcggatcac cgcgctcggc gaaaccctgc gcgcgggctt ggccgaacgc gccgccgccg
     3601 ccggcttcgg atttcgccag acgggccccg gcgtcatgcc gctgttcctg ttcgacgacg
     3661 acccggacct gcgcaagggc ttctgctggt ccagcgccat gctggccaag ggcgtctacg
     3721 tgcacccctg gcacaacatg ttcctctgcg cggcgatgac tgacgcggac atccagttcg
     3781 ccctcgacgc ggccgaacag gcgtttcaaa ctctgaagac gcaggcgcgc gacctgccgc
     3841 cggtggagaa gctcgccttc ctcgccgcgc gataagacgg caagggagat cgggatgggg
     3901 ttcgaggctt acgcggacta tgacggcctg gggctggccg atctggtgaa ccggggcgag
     3961 gtctcgccgg ccgagctggt cgaggcggcc atcgagcgga tcgagcgcca caacccgacc
     4021 ctcaacgccg tggtgcacaa ggcctatgac gaggcgcgca aggtcgccgc cgggccgctg
     4081 cccgacggtc cgttcaaggg cgtgccgttc ctgatcaagg acctcggcgc caaggtcgtc
     4141 ggctggccgc gcacctcggg ctccaactac gccgacgtgc ccgccgacag cgaggacagc
     4201 gtgctggtcc gccgctaccg cgagagcggc gtggtgttgc tcggcaagac caacacgccc
     4261 gaatacggca tcccgggcgt gacccagtcc gcgcggctgg ggccctgcgg caatccctgg
     4321 aatccggaac acatcagcgg cggctcgtcc ggcggggcgg ccagcgccac ggcctcgggc
     4381 atggtccccc tggcccacgc cagcgacggc ctgggctcga tccgcattcc ggcggccaac
     4441 tgtggtctgg tcggcatgaa gatcacccgc gaccgcaccc cgatctgcga ggtgaccgac
     4501 ggcgccatgg gcttctcggt ccaccatgtg gtcagccgca ccgtgcgcga cagcgccgcc
     4561 atgctcgacg ccaccgacgg accccagccg ggcgatccct tcacccaccc ccgcaaggag
     4621 cgcccgttcc ttgaggaggt cagccgcagc cccggcaagc tgcgcatcgc ctggtccaac
     4681 gaaaccccca gcggccggcc gatgggcgac gagatgatcg ccgcgacgca gaagacggtc
     4741 gagaccctca aggcgctcgg ccacgaggtg ttcgagatgg gcctcggcat cgactaccgc
     4801 ctgatgtacc gcgcccaggg cctggtctcg gcctcgaact tcgccgccac catgatgcgc
     4861 cgcgccgaga agttcggccg cgaaccggag ccgggcgaac tcggccccct ggcccagcgc
     4921 ggctgggacg gcggccgccg cgtcaccggc cagcaggcgc tgtggggctg gcagaccctg
     4981 cggctgatca acatccagat cctcgagcgc ttccaggcct tcgacgtctt cctgacgccc
     5041 acgatgagct atccctcgcc gcgcatcgac tggctcgacc ccctgaccgt ggacgtgaag
     5101 gagttcgaca agcgccaggc cgccacctac ggcaccaccc cgccgttcaa cttcaccggc
     5161 cagccgtcgc tgtcgctgcc gctgtggcag tcggaggcca acctgccgct cggcatgatg
     5221 ttcaccggcc gcttcggcga cgaggcgacg ctctatcgcc tcgccggcca gctggagaag
     5281 gaaatcccct ggatcggccg caagccgccg gtgtggggtt agccggggac atgtaccgcc
     5341 aggtacgtgt cccctaatcg agccatcgct gaatctggat tgggggacac gtaccttcgg
     5401 tacatgtccc cgaggagccc cgcatgaaag ccgccgtcta ctacgagaac ggatcccccg
     5461 atgtcctgaa gtacgaggat gtgccggatc ccgtctgcca tcccaagggc gtgattatcc
     5521 gggtgctggc ggtcagcatc gagggcggcg acaccctgaa ccgctggcgc gggccgctgg
     5581 tcaccaggcc gcacattgtc ggctaccagg cgtccggcga gatcgtcgag gtcgggtccg
     5641 aagtcaccca tctcaaggtc ggccagaagg tcgccaccgt cgacggcttt ggcagccacg
     5701 ccgagctccg ccccgtgccg gcccgcaact gctggccgat cccggacagc ctcgatccgc
     5761 gccacgccgc tgtcatcccc gtggccttcg gcacggccca cgactgcctg ttcgagttcg
     5821 gccgcctgaa ggccggcgag accgtgctgg tccaggccgg cgcctccggc gtgggcctgg
     5881 ccgccatcca gctggccaag cgggcagggg cgacggtgct ggccaccgcc tccagcgacg
     5941 cgcggctgga gaagctcaag gcgtatgggc tggaccacgg gatcaactac acgaaggacg
     6001 ccgtgcccga ggcggtgatg aagatcaccg acaagaaggg cgtgaacctg gtggtcgaca
     6061 gcgtcggcgg cccgaccctg cagggctcga tcctgtcgct cggctaccgc ggccgggtgt
     6121 cgatggtcgg ccaggccggc cgcgagccga tggtcgtcga cgtcggcagc ctgatgggca
     6181 agaacggctc cctcagcggc gtcttcctcg gcgccgagat catgaccgac cgcgcccacg
     6241 acatgatcca gggcctgatc gaggacgcgg cgaagggcga gatcaaggtg gtcatcgacc
     6301 gggagttccc actgtcggaa gccgcagccg cgcacgcgta catcgagagc cggcaagcgg
     6361 taggacgggt gctgttgatc ccgtgatccc tgacctctcc ctcttgggag agggggacca
     6421 tgcgaagcat ggtggagtgg gagtctcagg tggtcgccaa ctacccaccg ccagagccgc
     6481 ctcgcttcga atgctcctgc cgccaccgcg catcccactc caccagcctg cggctggtcc
     6541 ccctctccca ggagggagag gtaagcttcc gcattggcct cggttcccca tatgcccccc
     6601 atgaccccgc tctccgttct cgacctcgcc cccgtcgttc agggctccac cgtgcagcag
     6661 gcgctgcaca acagccggga cctggcgcag cacgcggagc ggtggggcta caaccggttc
     6721 tggctggccg agcatcacaa catgcgcggc atcgccagcg ccgcgaccag cgtgctgatc
     6781 ggccatgtcg ccgagggcac ctcgacgatc cgcgtcgggt cgggcgggat catgctgccc
     6841 aaccatgcgc cgctgcagat cgccgagcag ttcggcaccc tggcggcgct gtacggtccg
     6901 cgcatcgacc taggcctcgg ccgcgcgccg gggaccgacc agatcaccgc ccgcgccctg
     6961 cgccggacca tggtcggcga cgtcgaccgc ttcccggccg atgtcgtcga gctgatgcgc
     7021 tacttccagc cggcccagcc cggccaggcg gtggtggcga cgcccgggga ggggctggag
     7081 gtgccgatct ggatgctcgg ctccagccag ttcggggccc atctcgccgc cgcgctcggc
     7141 ctgccctacg ccttcgccag ccacttcgcg ccgcaggaga tggaaacggc ggtggccgcc
     7201 taccgcgcgc ggttcgagcc ctcggcccag cgcgacaagc cctatgtcat gctggccctc
     7261 aacgtcttcg ccgccgacac ggacgaggag gcccgcctgc tgttcacctc gctgcagcag
     7321 gctttcgtca acctcgccac cggcatgccc ggccgactgc cgccgccgat ggagggctat
     7381 gaggcgcgac ttcccgccga cgcgaaggcg cggctggcct cggccctgcg ctgttcggtg
     7441 gtcggctcgc gcgagacggt ccgcaagggc ctggccgagt tcatcgagcg gaccggcgct
     7501 gacgagctga tcctcaccgg ccagatccac gatcacgccg cccgcctgcg cagcttcgag
     7561 atcgcggcgg aggtggcccg cgagctggcc gacgcggcgt agcggtcagc gagcggggac
     7621 atgtaccgcc ttcggtacgt gtcccctaaa ccgtggcctc ccgattgggg gacacgtacc
     7681 ttcggtacat gtcccctaga cccgcgtgat cctccagaac cgccaggtca tcgccgccgc
     7741 ggccacggcc aggccgaagg ccaggccgat ccagatgccc aggccctgca tcttcagccc
     7801 cacgccgagc aggatgcaga ccggcgcccc ggccagccag tagctgaccc cggccaggac
     7861 catcggcatg cgcgcgtcct tcaggccgcg cagcgcctgg gcgccgacca cctgcagggc
     7921 gtcgaacacc tggaaggccg ccgcgaccag caggaactgc gtggccagca cgaccaccgc
     7981 cccgtcgccg gcccggccgt ggccgaggta gaggccggcg atctgcgggc cgaacagcac
     8041 catggccac
//
LOCUS       DAORNX010000082         8032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6623_length_8032_cov_7.792779, whole genome shotgun sequence.
ACCESSION   DAORNX010000082 DAORNX010000000
VERSION     DAORNX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6623_length_8032_cov_7.792779"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1137)
                     /locus_tag="PLO65_05275"
     CDS             complement(52..1137)
                     /locus_tag="PLO65_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD27693.1"
                     /translation="MAEAAAAPTAQDLNSGTKPVEERHRIDEARLAAWLEANVEGYAG
                     PLEVRQFKGGQSNPTYQLVTPTRRYVLRRKPPGKLLPSAHAVDREFKVISALGKTGFP
                     VARAYALCEDDDVIGTMFYVMDNVEGRILWDLTLPDYQPAERRPIYEAKVATLAALHN
                     TDYEAIGLGDFGRPGNYFSRQIDRWIKQYKASETQSIASMNRLMEWLPATPPEDDKTS
                     IVHGDYRLDNMILHPTEPRVVAVLDWELSTLGNPMADFSYLLMHWVMPGAERASLSGI
                     GDYKAWGIPTIDEVVEQYCGLTGRSGVPNLDWYFAYNLFRLAGICQGIVGRVRDGTAA
                     SAHAATMEARVPVLADAALSFAIKAGA"
     gene            complement(1218..1343)
                     /locus_tag="PLO65_05280"
     CDS             complement(1218..1343)
                     /locus_tag="PLO65_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27694.1"
                     /translation="MKFNLLRFVRCVLGRSHDYKNSRSRRGFATCQKCGHRKRWR"
     gene            complement(1437..>1937)
                     /locus_tag="PLO65_05285"
     CDS             complement(1437..>1937)
                     /locus_tag="PLO65_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRD27695.1"
                     /translation="AEGTPYRGVIYAGLMLTPGGPRLVEFNARFGDPECQVLMLRLES
                     DIVPYLAAAAGGTLASLPPPAWRDEAALCVVIAADGYPQSPIAGAIIRGADGPDRDGA
                     VIFHAGTTRDPDGTLRAAGGRVLNVCARAPRLAEARDRAYARIGQVDYPGGFFRSDIG
                     WRALST"
     assembly_gap    1938..2032
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2033..2705)
                     /locus_tag="PLO65_05290"
     CDS             complement(<2033..2705)
                     /locus_tag="PLO65_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRD27696.1"
                     /translation="MNILLVGSGGREHALAWKIKQSPLLTRLVAAPGNPGIAGLCEIR
                     PVPATDVDGLVALAREMAADLVVVGPESALEVGLADRLAQAGIPCFGPTAAAARLETS
                     KAFSKDFCARHNLPTAAYGVFETTQDAAAFLDGQSPPFVIKADGLAAGKGVVIAADRA
                     EADAAVADMLGGRFGQAGARVVIEEFMPGEEVSLFALCDGETAVPFGYAQDHKRAYDG
                     DLGPNT"
     gene            complement(2779..3468)
                     /gene="phoB"
                     /locus_tag="PLO65_05295"
     CDS             complement(2779..3468)
                     /gene="phoB"
                     /locus_tag="PLO65_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRD27697.1"
                     /translation="MTPTVLVVEDEDALATLLQYNLEKEGYRVVHAGDGEEALILVQE
                     ELPDLVVLDWMLPKVSGIEVCRRLRQRPESRNLPILMLTARGEESDRVRGLDTGADDY
                     VVKPFAMSELTARIRAVMRRIRPGLADDRVTCGDIVIDRVAHRVKRGGHEVHLGPTEF
                     RLLDHLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNGKADADPIRTV
                     RSAGYSLDLDG"
     gene            complement(3465..4157)
                     /gene="phoU"
                     /locus_tag="PLO65_05300"
     CDS             complement(3465..4157)
                     /gene="phoU"
                     /locus_tag="PLO65_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRD27698.1"
                     /translation="MNEHIVRSYEEELNQLAAEVVRMGGLAEAQVNDSIHAFARRDVP
                     LAQAMIGRDGKLDAMEADIERAAIRMIALRQPMANDLRRTMSAMKIAMNLERCGDLAK
                     NIGKRTLILAEAEPAGPVTRSIERMGRLVASRLKEVIDAYTASEVDRAVAVWTRDTEV
                     DEHYESLFRELLTYMMGDPRTITAGAHLLFVAKNLERIGDHATNIAEIVHFEITGREI
                     VSERPKLDTLTP"
     gene            complement(4166..4987)
                     /gene="pstB"
                     /locus_tag="PLO65_05305"
     CDS             complement(4166..4987)
                     /gene="pstB"
                     /locus_tag="PLO65_05305"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRD27699.1"
                     /translation="MTAQPPLIRTAAPHSLNAAPVNPAKIQVRDVSVHYGAKQALFDV
                     TLDIPGKAVTAFIGPSGCGKSTFLRCINRMNDTIPGCRVDGRIEMDGEDVNDSHIDPV
                     VLRAKVGMVFQKPNPFPKSIFENVAYGPRIHGIASSKSELEGIVEASLKKAGLWNEVS
                     DRLNAPGTGLSGGQQQRLVIARAIAVNPEVILMDEPCSALDPIATAKIEELIDDLRQQ
                     FCIVIVTHSMAQAARVSQRTAFFHMGRLIETGSTEEIFTNPRESRTQDYITGRIG"
     gene            complement(4993..6252)
                     /gene="pstA"
                     /locus_tag="PLO65_05310"
     CDS             complement(4993..6252)
                     /gene="pstA"
                     /locus_tag="PLO65_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRD27700.1"
                     /translation="MTDAAASRRLKKRHAAEARFRLYGRLAIIVALSFLVLLLGRIGL
                     QGYSTFTTNTVSLPVYLDPARIDRTDLEGSNYDYVVALALLKRMGETEDDLGLASADA
                     MALVSRDLGFQILDKVKADPGLIGKTITVTGPVKADADLYYKGELKRSTDEADRKLND
                     RQLGWLDRLKADGAVTSGFNVNFFTQADSTEPEQAGILGAVIGSAMMLLVTALIAVPM
                     GVMAAVWLEEFAPKNRWTDIIEVNINNLAAVPSIVYGLLGLAVFINGFGAPRGAPITG
                     GMVLALMALPTVIIATRSALKAVPPSIREAAWGVGASRTQTVFHHVLPQAMPGVMTGT
                     ILSLAHALGETAPLLMIGMIAFNPGLPQGFTSSASVLPVQVFIWENASERAFHERTAA
                     AIIVLLVFMIAMNAAAVILRRRFERRW"
     gene            complement(6245..7687)
                     /gene="pstC"
                     /locus_tag="PLO65_05315"
     CDS             complement(6245..7687)
                     /gene="pstC"
                     /locus_tag="PLO65_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRD27701.1"
                     /translation="MTYLIALALLAAFSAGVYLLGRRRAVALAGGRSASLHSLPGYHG
                     VWVALWAGVPAALIVILFAIFGGRLEEAALRADVPAAVAGLPAERQEVFWSDAAAVAR
                     GQSSSEIVYEGELRAALDAKAAEGRRISAVGLAAVLGLSGVLALCGFLLAFPRLSKDF
                     RARNRVEGWTNILLIACSTIAVMTTLGILLSLAWESYRFFESVPPLDFLLGTAWDPQI
                     AMRSDQVAAEGAFGAVPLFAGTFLIMLIAMAVAAPIGLYSAIYLSEYAGPATRATIKP
                     LLEILAGVPTVVYGFFAALTVGPTFRAFFNWIGGFLVGGPMDGLGQYLETVQNQMALT
                     AGVVMGIMLIPFVSSLSDDIINAVPQTLRDGSYAMGATRSETVKRVILPAALPGVVGA
                     LMLAVSRAIGETMIVTMAAGLQARLTANPLDTVTTVTVQIVTLLTGDQEFDSPKTLAA
                     FGLGLTLFTVTLALNIFALGIVRKYREQYD"
     assembly_gap    7729..7801
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7802..>8032)
                     /locus_tag="PLO65_05320"
     CDS             complement(<7802..>8032)
                     /locus_tag="PLO65_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27702.1"
                     /translation="YALLQVVDAGPGLPREALPRLTERFYRVEGQKSGDRSGTGLGLA
                     IVKHIVNRHRGGLSVESAEGRGATFSVWLPLAA"
BASE COUNT         1224 a         2572 c         2841 g         1227 t
ORIGIN      
        1 ccagcagggc cccggcggcc agggctgcgg cgcgcttgat ggcgacggtc atcaggcgcc
       61 cgccttgatg gcgaagctca gggcggcgtc ggcgagtacg ggcacgcggg cctccattgt
      121 tgcggcatgg gcgctggcgg cggtgccgtc gcgcacgcgg ccgacgatcc cctggcagat
      181 gccggccagt cggaacaggt tgtaggcgaa ataccagtcg aggttgggca cgcccgagcg
      241 tcccgtcagc ccgcagtact gctcgaccac ctcgtcgatc gtcggaatac cccaggcctt
      301 gtagtcaccg atcccggaca gggaggcgcg ctcggcgccg ggcatcaccc agtgcatgag
      361 caggtaggag aagtcggcca tcgggttgcc cagcgtcgac agctcccagt cgagcaccgc
      421 cacgaccctg ggttcggtcg ggtgcaggat catgttgtcc agccgatagt cgccgtgaac
      481 gatcgaggtc ttgtcatcct cgggcggggt ggccggcagc cactccatca gccggttcat
      541 cgaggcgatc gattgcgtct ccgatgcctt gtactgcttg atccagcggt cgatctggcg
      601 gctgaaatag ttgcccggac ggccgaaatc gcccaggccg atggcctcgt agtcggtgtt
      661 gtgcagggcg gccagggtgg ccaccttggc ctcgtaaatc ggccgccgct cggccggctg
      721 gtagtccgga agggtcaggt cccagaggat ccggccctcg acgttgtcca tcacgtagaa
      781 catcgtgccg atgacgtcgt cgtcctcgca gagggcgtag gcccgggcca ccgggaagcc
      841 ggtcttgccg agcgcggaga tgaccttgaa ctcgcggtcg acggcatgcg ccgacggcag
      901 cagcttgccc ggcggcttgc gacgcaggac gtagcgccgg gtcggcgtga cgagctggta
      961 ggtcggattg gactggccgc ccttgaactg gcggacctcg agggggccgg catagccctc
     1021 gacattggcc tcgagccagg cggcgagccg ggcttcgtcg atgcgatgcc gctcctcgac
     1081 cggcttggtg ccggagttga gatcctgagc ggtcggcgcg gcggcggcct cggccatgaa
     1141 cgtttccccg tattcgtacg cttgtttgag gcggcgagtt taggggttgt cggcggcggc
     1201 gcaaggacgc cttggcgtca gcgccatcgc ttgcgatgac cgcatttctg gcaggtggcg
     1261 aagccgcgcc ggctgcggct gttcttgtag tcgtgactgc gaccaaggac gcagcggacg
     1321 aaccgcagca gattgaattt catccaacgc cccctcaagc gcacgatctc aaccccagat
     1381 atgcgcagag gagtaggggc gattcaattg cccaaattga gcaggatgcc gacgattcag
     1441 gtactcaggg ctcgccaacc aatgtccgag cggaaaaagc cgcctggata gtctacctgg
     1501 ccgatccggg cgtaggcgcg gtcgcgggct tcggcgagac ggggcgccct ggcgcagaca
     1561 ttgagcaccc gtcccccggc ggcccgcagg gtcccgtccg ggtcgcgcgt cgtgccggca
     1621 tggaagatca ccgcgccgtc ccggtcgggc ccgtcggcgc cccggatgat cgccccggcg
     1681 atcgggctct ggggatagcc gtcggccgcg atgacgacgc acagcgccgc ctcgtcgcgc
     1741 caggccggcg gcggcaggga cgcgagcgtc ccgcccgcgg cggccgcgag gtaggggacg
     1801 atgtcggact ccagccgcag catcagcacc tggcactccg ggtcgccgaa ccgcgcgttg
     1861 aactccacca gccgcgggcc gcccggggtc agcatcaggc cggcatagat cactccgcga
     1921 tagggcgttc cctccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggtgttg
     2041 gggcccagat cgccgtcgta ggcgcgcttg tggtcctggg catagccgaa gggcacggcc
     2101 gtctcgccgt cgcagagggc gaacaggctg acctcctcgc cgggcatgaa ctcctcgatg
     2161 accacgcgcg cgccggcctg gccgaaccgg ccgcccagca tgtcggccac cgcggcgtca
     2221 gcctcggccc ggtcggcggc gatgaccacg cccttgccgg cggccaggcc gtcggccttg
     2281 atcacgaacg gtggcgactg gccgtcgaga aaggcggccg cgtcctgcgt cgtctcgaac
     2341 acgccatagg cggcggtcgg caggttgtgg cgcgcgcaga agtccttgct gaacgccttg
     2401 gaggtctcca gccgcgccgc cgcggcggtc gggccgaaac aggggatgcc ggcctgcgcc
     2461 aggcgatcgg ccaggccgac ctcaagcgcc gattcgggtc cgaccacgac caggtcggcg
     2521 gccatctcgc gcgccagggc gaccagcccg tcgacgtcgg tcgccgggac cggccggatt
     2581 tcgcaaaggc cagcgatgcc cgggttgccg ggcgccgcga ccagccgcgt cagcagcggc
     2641 gactgtttga tcttccaggc cagcgcgtgt tcacggccgc ccgatccgac gagaaggatg
     2701 ttcatgggcg ggttatgcgc gtgcggcgaa cagggtcaag cgggcgaggc ctctctcccc
     2761 ttgaaggggg gaggggcctc agccgtccag atccagcgaa tagccggccg agcgcacggt
     2821 gcggatcggg tcggcgtcgg ccttgccgtt gagcgccttg cgcaggcggc cgatgtggac
     2881 gtcgaccgtg cgcgcctcga catagacgtc cgagccccag accgcgtcga gcagctgctc
     2941 gcggctgaac acccgacccg gatgctgcat cagatggtcg agcaggcgga actcggtcgg
     3001 gcccaggtgg acttcgtggc cgccgcgctt cacccggtgg gcgacgcggt cgatgacgat
     3061 gtcgccgcag gtcacgcgat cgtcggccag gcccggacga atgcgccgca tcaccgcgcg
     3121 gatccgggcg gtcagctcgc tcatggcgaa cggcttgacg acatagtcgt cggccccggt
     3181 gtcgagcccg cgcacgcggt cgctctcctc gccacgggcg gtcagcatca ggatcggcag
     3241 gttgcggctc tccgggcgct ggcgcaggcg gcggcagacc tcgatgcccg acaccttggg
     3301 cagcatccag tcgagcacca ccaggtcggg cagctcctct tggaccagga tcagcgcctc
     3361 ctccccgtcg ccggcgtgga ccacgcggta gccttccttc tcgagattgt actgcagcag
     3421 ggtggctagg gcgtcctcgt cctcgaccac cagaaccgtg ggcgtcatgg cgtgagcgtg
     3481 tccagcttgg gccgttcgga gacgatctcc cggccggtga tctcgaaatg gacgatctcg
     3541 gcgatgttgg tggcgtggtc gccgatccgt tcgaggttct tggccacgaa caggaggtgg
     3601 gcgccggcgg tgatcgtgcg cggatcgccc atcatgtagg tcaacagttc gcggaacagg
     3661 ctttcgtagt gctcgtcgac ctcggtgtcg cgggtccaga cggctaccgc gcggtccacc
     3721 tccgaggccg tgtaggcgtc gatcacctcc ttgagtcgcg aggcgaccag ccggcccatc
     3781 cgttcgatcg aacgggtcac cggcccggcg ggttcggcct cggccaggat cagggtgcgc
     3841 ttgccgatgt tcttggccag gtccccgcag cgttccaggt tcatggcgat cttcatcgcc
     3901 gacatggtcc ggcgaaggtc gttggccatc ggctggcgca gggcgatcat gcggatggcg
     3961 gcgcgctcga tgtcggcctc catcgcgtcc agcttgccgt cgcgaccgat catcgcctgg
     4021 gccaggggga cgtcgcggcg ggcgaaggca tggatcgagt cgttgacctg ggcttcggcc
     4081 aggccgccca tgcggaccac ctccgcggcc agctggttca gttcttcttc gtaggatctg
     4141 acgatgtgtt cgttcatggc gcgcctcagc cgatccggcc ggtgatgtag tcctgggtcc
     4201 gcgactcgcg cggattggtg aagatctctt cggtcgagcc ggtctcgatc agccggccca
     4261 tgtggaagaa ggccgtgcgc tgcgagaccc gcgcggcctg ggccatcgag tgcgtgacga
     4321 tgacgatgca gaactgctgc cgcaggtcgt cgatcagctc ctcgatcttg gcggtggcga
     4381 tcgggtcgag ggccgagcag ggctcgtcca tcaggatcac ttcgggattc accgcgatgg
     4441 ccctggcgat gaccagccgc tgctgctgac cgccggacag accggtgccc ggcgcgttca
     4501 ggcggtcgga cacctcgttc cacaggccgg ccttcttcag cgaggcctcg acgatgcctt
     4561 ccagctcgct cttgctggag gcgatgccgt gaatgcgcgg gccgtaggcg acgttctcga
     4621 agatcgactt ggggaacggg ttgggcttct ggaacaccat tccgaccttg gcgcgcagga
     4681 ccaccgggtc gatgtgactg tcattcacgt cttcgccgtc catctcgatc cggccatcca
     4741 cgcggcagcc ggggatggtg tcgttcatcc ggttgatgca gcgcaggaag gtcgacttgc
     4801 cgcagccgga cgggccgatg aaggccgtca cggccttgcc cgggatgtcg agcgtgacgt
     4861 cgaacagggc ctgtttggcg ccgtagtgga cgctgacgtc ccgcacctgg atcttggccg
     4921 gattgacggg cgcggcgttg aggctgtgcg gcgcggccgt gcggatgaga ggcggttggg
     4981 cggtcatggg ggctaccatc tgcgctcgaa gcggcggcgc aggatcaccg cggccgcgtt
     5041 catcgcgatc atgaagacca gcagcacgat gatggctgcg gcggtgcgct cgtggaaagc
     5101 gcgttcggag gcgttttccc agatgaacac ctgcaccggc agcacgctgg cggaactggt
     5161 gaagccctgg ggcaggccgg ggttgaaggc gatcatgccg atcatcagca ggggcgccgt
     5221 ctcgcccagg gcgtgggcga gggacaggat cgtgccggtc atcaccccgg gcatggcctg
     5281 gggcaggacg tggtggaaca cggtctgggt gcgcgaggcg ccgacgcccc atgcggcctc
     5341 ccggatggag ggcggcacgg ccttgagcgc agagcgggtg gcgatgatca cggtcggcag
     5401 ggccatcagg gccagcacca tgccgcccgt gatcggcgcg ccgcgagggg cgccgaagcc
     5461 gttgatgaac accgccaggc cgagcaggcc gtagacgatc gacggaacgg ccgcgaggtt
     5521 gttgatgttg acctcgatga tgtcggtcca gcggttcttc ggcgcgaatt cctccagcca
     5581 gaccgcggcc atcaccccca tcggcaccgc gatcagggcg gtgaccagca gcatcatggc
     5641 cgagccgatc accgcgccga ggatgccggc ctgttccggc tcggtggaat cggcctgggt
     5701 gaagaagttg acgttgaagc cgctggtgac cgcgccgtcg gccttcagcc ggtcgagcca
     5761 gcccagctgg cgatcgttga gcttgcgatc ggcctcgtcg gtcgagcgtt tcagctcacc
     5821 cttgtagtag aggtccgcgt ccgccttgac cgggccggtc acggtgatcg tcttgccgat
     5881 caggcccgga tcggccttga ccttgtcgag gatctggaag cccaggtcgc ggctgaccag
     5941 ggccatggcg tccgcggacg ccagccccag gtcatcctcg gtctcgccca tccgcttgag
     6001 cagcgccaag gccacgacgt agtcgtagtt ggacccctcc agatcggtgc ggtcgatccg
     6061 cgccgggtcc aggtagaccg gcagggagac ggtgttggtg gtgaaggtcg aatagccctg
     6121 caggccgatg cggcccagca gcaggacgag gaacgacagc gccacgatga tcgccagccg
     6181 gccgtagagc cggaaccggg cctcggcggc gtggcgcttc ttcaggcgtc ttgaggcggc
     6241 ggcgtcagtc atattgttcg cggtacttcc gcacgatgcc cagggcgaag atgttgagcg
     6301 ccagggtgac ggtgaaaagg gtcaggccga ggccgaacgc ggccagggtc ttggggctgt
     6361 cgaattcctg gtccccggtc agcagggtga cgatctgcac ggtgacggtg gtcacggtgt
     6421 cgagcgggtt ggcggtcagc ctggcctgaa ggccggcggc catggtcacg atcatggtct
     6481 cgccgatggc gcgcgagacc gccagcatca gggcgccgac cacgccgggc agggcggccg
     6541 gcaggatgac ccgcttgacc gtctccgacc gggtcgcgcc catggcgtaa ctgccgtcgc
     6601 gcagggtttg cggcacggcg ttgatgatgt cgtccgacag ggacgagacg aacgggatca
     6661 gcatgatgcc catgaccacg cccgcggtca gggccatctg gttctggacg gtttccagat
     6721 actggccgag gccgtccatc ggtccgccga cgaggaaacc gccgatccag ttgaagaagg
     6781 cgcggaaggt cgggcccacg gtcagggcgg cgaagaagcc gtagaccacc gtcggcacgc
     6841 ccgcgagaat ctccagcagc ggcttgatgg tcgcccgcgt cgccggcccc gcgtactcgg
     6901 acagatagat cgccgagtag aggccgatcg gggcggcaac ggccatggcg atcagcatga
     6961 tcaggaaggt gccggcgaac agcggcacgg cgccgaacgc cccctcggcg gcgacctggt
     7021 cgctgcgcat ggcgatctgc ggatcccagg cggttccgag caggaaatcg agcggcggca
     7081 ccgattcgaa gaacctgtag ctctcccagg ccagggacag aaggatgccc agggtcgtca
     7141 tcaccgcgat ggttgagcag gcgatcaaca ggatgttggt ccagccctcg acccggtttc
     7201 gggcgcggaa atccttcgag aggcggggga aggccagcag gaagccgcac agggcgagca
     7261 cgcctgacag gcccagcacc gccgccagcc cgaccgcgct gatccggcgg ccctcggcgg
     7321 ccttggcgtc aagcgccgcg cggagctcac cctcgtagac gatctcgctg ctgctctgac
     7381 cccgggcgac cgcggcggcg tcgctccaga agacctcctg gcgctcggcc ggcaggccgg
     7441 cgaccgccgc cggaacatcg gcccgcaggg cggcctcctc gagacgtccg ccgaagatgg
     7501 cgaacaggat gacgatcagg gccgcgggaa ccccggccca cagcgccacc cagacgccgt
     7561 ggtagccggg cagggagtgc aggctggcgg acctgccgcc ggccagggcc accgcgcggc
     7621 gccggccgag caggtacacg ccggccgaga aggcggcgag cagggccagg gcgatcagat
     7681 aggtcatcgc gggtccgaaa agcgcatctg cggggttggc cggaacctnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 ncgcggccag cggcagccag actgaaaagg tcgcgccgcg gccttcggcg ctctcgaccg
     7861 agagcccgcc ccggtggcgg ttgacgatgt gcttgacgat ggccaggccc aggccggtgc
     7921 cggaacggtc gccgctcttc tggccctcga cccggtagaa gcgctcggtc agcctcggca
     7981 gggcctcgcg gggcaggccc ggtccggcgt cgacgacctg cagcagggcg ta
//
LOCUS       DAORNX010000083         8021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6635_length_8021_cov_7.025358, whole genome shotgun sequence.
ACCESSION   DAORNX010000083 DAORNX010000000
VERSION     DAORNX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_8021_cov_7.025358"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLO65_05325"
     CDS             complement(<1..247)
                     /locus_tag="PLO65_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27703.1"
                     /translation="MSADPLDRVAAIQWFARQPAARRDALVRSGRLTRRNAGEWMHGE
                     GDEETGLLAVVEGALKVYGQAPGGREALISLLPPGTVI"
     gene            325..1167
                     /locus_tag="PLO65_05330"
     CDS             325..1167
                     /locus_tag="PLO65_05330"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD27704.1"
                     /translation="MIDTQLRIDPLPGCGAQVKAVDLADLTDKEVDGLRQALFDHGVL
                     IFRGQSLTPEQHIALAERIAPIDVNRFFPAAPGYPVIAKVEKTTEQTTNIGGGWHTDH
                     SYDIAPAMGSVLVARELPPSGGDTLFADMYAAYETLDAATKAEIAGLRAVHGSDHVFG
                     EKGVYAQTDQPELAKGGVETPETVHPVVIKHPGSGKPVLYVNPTFTLRFEGMTREESM
                     PLLMKLYAHAMDTSRVHTLVWEPGTVAIWDNRATWHFAKNDYHGHYRLMHRITLAGCA
                     LEAA"
     gene            complement(1201..1779)
                     /locus_tag="PLO65_05335"
     CDS             complement(1201..1779)
                     /locus_tag="PLO65_05335"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRD27705.1"
                     /translation="MTSRRTVLAGLTVASLAGPALAGKPFTPLFNGADLDGWTPLGDA
                     NWTVEGGVLSADRGAMSFLVSRDSFRDFDLRAELWVSADANSGVFIRCSDRAVITPAT
                     AYEVNVFDARPDPTYGTGAIVDVAPVSPMQKAGGRWNLMEVRARGDRLWVVFNGVKTV
                     DGVRNAAHSEGPIALQYGSGVVKVRAVEVRAV"
     gene            complement(1855..2403)
                     /locus_tag="PLO65_05340"
     CDS             complement(1855..2403)
                     /locus_tag="PLO65_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27706.1"
                     /translation="MPVIGSERLAARWRIAVWGGAALLLFLPLVAMQFTTEVAWTGSD
                     FLVFGVMLTILCGGVELAVRMSRRRAYRAASGIALVAGFLLVWMNLAVGIIGSEENPL
                     NLMYGGVLVVGLLGALLARFRPAGMVRTMAAMAAAQVVVAAVAQAAGHFTWVLTAVFV
                     AAWLASAWLYRKAARDLDAAVT"
     gene            complement(2496..3134)
                     /locus_tag="PLO65_05345"
     CDS             complement(2496..3134)
                     /locus_tag="PLO65_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27707.1"
                     /translation="MMRLRLILALVLAPLASPAMAAKPATEYAGADGGYLAYAVGDIA
                     LGMHFSFPYRRVALPDGAPVSDWKGEIAPRLGGAIYLKVKNPDFEGRETGHVVVRRLP
                     PGEYLIENFAFYGSSPTGSAYAWSSAKPFAIRFRIEPGKATYIGSFMRAPSPGPGVVP
                     GIGPAGFFVIADRSARDLPIARPRLPGACEVVSQVTNVDAFGSAILRSREPD"
     gene            complement(3131..3826)
                     /locus_tag="PLO65_05350"
     CDS             complement(3131..3826)
                     /locus_tag="PLO65_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27708.1"
                     /translation="MLRPFADNLWIADGPVADVAGFRYPTRMAVMRLAGGGLFVWSPT
                     ALDDALKAEIDALGPVRHVVAPNALHHLHIDSWRQACLEARFTAPPGLRGKRPDLRFD
                     DDLADGSVHVWSADIDHVLVHGNLITTEVVFFHRASRTVLITDLIQQFSPGWFTGWRG
                     IVARLDLMTGPRPEVPRKFRNTFIDRRAAREAIRRVLAWPAERVLMAHAPPVESNGQA
                     FLRMTFEWLGVDS"
     gene            complement(3979..5241)
                     /gene="clpX"
                     /locus_tag="PLO65_05355"
     CDS             complement(3979..5241)
                     /gene="clpX"
                     /locus_tag="PLO65_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRD27709.1"
                     /translation="MTKAASGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREEHKIAFVKSKDGVPTPKEIREVLDDYVIGQDHAKKVLSVAVHNHYKRLNHAQ
                     KNNDVELAKSNIMLVGPTGSGKTLLAQTLARIIDVPFTMADATTLTEAGYVGEDVENI
                     VLKLLQSADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFIVGGAFAGLEKIISGRGAGTSIGFGAKVADPD
                     ERRTGEILRQVEPEDMLRFGLIPEFIGRLPVIATLEDLDEEALVKILTEPKNALIKQY
                     ARLFEMENVGLKFTDDALSAVARKAIARKTGARGLRSIMEAILLETMFELPSYDGVEE
                     VVVNGEVVEGRAQPLVIYAEKKAGGASA"
     gene            5596..6264
                     /locus_tag="PLO65_05360"
     CDS             5596..6264
                     /locus_tag="PLO65_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRD27710.1"
                     /translation="MGRRSLLAQGLSASRPVMPDPLEAPALVAFDFDGTLTVEDSFTA
                     FLKWRAGPLRWAFGCLRLTPAAIGYLFHRNRGRMKSAAIREFLKGTPREKLEAQARTF
                     AELAAPRLFRPDALMTWRRWRANGARTIIVSASPDLIVAPFARGLGCDRLIASAIAFD
                     RDDRVIGILAGPNCRGPEKVRRLREAYGEDVHLTAAYGDTSGDREMLRIADEKGYRVF
                     KGRP"
     gene            6324..6581
                     /locus_tag="PLO65_05365"
     CDS             6324..6581
                     /locus_tag="PLO65_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27711.1"
                     /translation="MKHAGPAALDQLEPLLAAIRALPGLKEKTRGVFYRGGRAFLHFH
                     EDSAGLFADVRPAGPDFERFRVETEAEQGAFVALVTVKLTA"
     gene            complement(6638..7303)
                     /locus_tag="PLO65_05370"
     CDS             complement(6638..7303)
                     /locus_tag="PLO65_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRD27712.1"
                     /translation="MSDYYRTISSSDPVQTALNLVPMVVEQTSRGERAFDIFSRLLKE
                     RIIFLTGPVEDGMSALICSQLLFLESENPKKEISMYINSPGGVVTSGLAIYDTMQYIK
                     SPVSTVCMGMAASMGSLLLCAGAAGQRIALPNARIMVHQPSGGFRGQASDIMRHAEDI
                     LATKKRLNEIYVKHTGRTYDEVETTLDRDHFMSAEDAKAWGIVDHVYESREAAEGAAG
                     EKS"
     gene            complement(7441..>8021)
                     /locus_tag="PLO65_05375"
     CDS             complement(7441..>8021)
                     /locus_tag="PLO65_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27713.1"
                     /translation="TDDSQALARSVVDSRPAAFARRERGMSRGDRALKAFVETPAPVR
                     WWHVTRTKTAEGESYTQGSGVRVRSASRLRATTRDDFDHVIILVDVRRIGVIRFEALA
                     DYITMIGLAQIEPDIDLGKTQSILGLFAARDRGAEPADGLTEWDAAYLLGLYGANRDV
                     VRGSRQEREIAQDMLRRLGPKAASAPAPPAGP"
BASE COUNT         1284 a         2781 c         2711 g         1245 t
ORIGIN      
        1 cgatcaccgt accgggcggc agcaggctga tcagcgcctc ccgcccgccc ggggcctggc
       61 cgtagacctt gagcgcgccc tcgaccacgg ccagcagccc ggtctcctcg tcgccctcgc
      121 cgtgcatcca ttcgccggcg ttccggcgcg tcaggcggcc cgagcggacc agcgcgtcgc
      181 ggcgcgcggc aggctggcgc gcgaaccact ggatggccgc gacgcgatcg agggggtcag
      241 cgctcatgtc accagtgtaa ctcccgtgac attccggacg ccagacctgt ggctcactgg
      301 cggcgacaaa ccgggagacg cgccatgatc gacacccaac ttcgcatcga ccccctgccc
      361 ggctgcggcg cgcaggtgaa ggccgtcgac ctggccgacc tgaccgacaa ggaggtcgac
      421 ggcctgcgcc aggccctgtt cgaccacggc gtgctcatct tccgtggcca gagcctgacc
      481 cccgaacaac atatcgcgct ggccgagcgg atcgccccga tcgacgtcaa tcgcttcttc
      541 ccggccgcgc ctggataccc tgtgatcgcc aaggtcgaga agaccaccga gcagaccacc
      601 aacatcggtg gcggctggca caccgaccac agctatgaca tcgcgccggc catgggctcg
      661 gtgctggtcg cccgcgagct gccgccgagc ggcggcgaca ccctgttcgc cgacatgtac
      721 gccgcctacg agacgctcga tgccgcgacc aaggccgaga tcgccggcct gcgggcggtg
      781 cacggctccg accacgtgtt cggcgagaag ggtgtatacg cccagaccga ccagcccgag
      841 ctggccaagg gcggggtcga gacgccggaa accgtccatc ctgtggtcat caaacacccc
      901 ggcagcggca agccggtgct gtatgtgaac cccaccttca ccctccgctt cgagggcatg
      961 acccgcgagg agagcatgcc gttgctgatg aagctctacg cccacgcgat ggacacgagc
     1021 cgcgtccaca ccctggtctg ggagcccggc acggtggcca tctgggacaa ccgcgcgacc
     1081 tggcatttcg ccaagaacga ctaccacggc cactaccggc tgatgcaccg catcaccctc
     1141 gccggctgcg cgctggaggc ggcgtaggcg cggagacagg cgcccggcgc ggatctcccg
     1201 tcagaccgcc cggacctcga ccgccctgac cttcaccaca cccgatccgt actgcagggc
     1261 gatcggccct tccgaatggg cggcgttccg gaccccgtca acggtcttca cgccgttgaa
     1321 gacgacccac aaccggtcgc cccgggcgcg aacctccatc aggttccagc ggccgccggc
     1381 cttctgcatc ggcgagaccg gcgcgacatc gacgatggcg ccggtgccgt aggtcgggtc
     1441 gggccgggcg tcgaacacat tgacttcgta ggccgtcgcc ggggtgatca ccgcgcggtc
     1501 gctgcagcgg atgaacacgc ccgagttcgc gtcggcgctg acccacagtt cggcgcgcag
     1561 gtcgaagtcg cggaaactgt cgcgcgacac caggaagctc atcgcccccc tgtcggcgga
     1621 caagaccccg ccctcgaccg tccagttggc gtcgccgaga ggcgtccagc cgtccagatc
     1681 cgcgccgttg aacaggggcg tgaacggctt gccggccagc gccggtccgg cgagcgaagc
     1741 gaccgtcagg ccggccagga cggtgcggcg gctggtcatg cggactcctg ttgaggcgcg
     1801 atcggcccag ttaggggcat cgccgggagt cgggcaaccg gcctccgcga tgagtcaggt
     1861 caccgcggcg tccaggtccc gggccgcctt gcggtacagc caggccgagg ccagccaggc
     1921 ggcgacgaac acggccgtca gcacccaggt gaaatgcccg gccgcctggg cgacggcggc
     1981 gaccaccacc tgcgcggccg ccatggccgc catggtccgg accatgccgg ccggacgaaa
     2041 gcgcgccagc agggcgccga gcaggccgac gacgagcacc ccgccgtaca tcaggttgag
     2101 cgggttctcc tcgctgccga tgatgcccac ggcgaggttc atccagacga gcaggaagcc
     2161 ggcgacaagg gcgatgccgg acgccgcgcg ataggcgcgg cgccgcgaca tccggacggc
     2221 cagctcgacg ccgccgcaga ggatcgtcag catgacgccg aagaccagga agtcggaacc
     2281 ggtccaggct acctcggtgg tgaactgcat ggcgaccagc ggcaggaaga ggagcagcgc
     2341 cgccccgccc cagaccgcga tccgccagcg agccgcgaga cgttcagaac cgatgacagg
     2401 catggcgaca tttctcccct tgagccccct gtatcgcatc gcgcgcgtgc agggccatgg
     2461 cggcaagctg tgtcgtttcg atgcagacgg cgccgtcagt ccggctcgcg gctgcgcaat
     2521 atcgcgctgc cgaaggcatc gacgttcgtg acctgggaga cgacctcgca ggcgccgggc
     2581 agccgcggcc tggcgatcgg caggtcacgc gccgatcggt cggcgatgac gaagaaaccg
     2641 gctggaccaa tgcccgggac gacacccggt ccgggcgacg gcgcccgcat gaagctgccg
     2701 atgtaggtcg ccttcccggg ctcgatgcgg aagcggatcg cgaacggctt cgccgacgac
     2761 cacgcatagg ccgatcccgt ggggctggag ccatagaagg cgaagttctc gatcaggtac
     2821 tcgcccggcg gcaggcggcg gacgacgaca tggccggtct cgcggccctc gaagtcgggg
     2881 ttcttgacct tgaggtagat ggcgccgcca aggcgcgggg caatctcgcc cttccagtca
     2941 ctgaccggcg cgccgtccgg aagcgccacg cggcggtagg ggaaactgaa gtgcatgccc
     3001 agggcgatgt cgccaacggc gtaggccagg tagccgccgt cggcgccggc gtactccgtg
     3061 gccggcttcg ccgccatcgc ggggctggcc agcggcgcga ggaccagggc caggatcagt
     3121 cggagccgca tcatgaatcc actcccaacc attcaaacgt catgcggagg aaagcctggc
     3181 cattcgactc cacgggcggc gcgtgggcca tcagcacccg ctcggcgggc caggccagaa
     3241 cgcgccggat ggcctcgcgc gccgcccgtc ggtcgatgaa ggtgttgcgg aacttgcgtg
     3301 ggacttccgg ccgcggcccg gtcatcaggt ccagccgggc gacgatcccg cgccagcccg
     3361 tgaaccagcc cgggctgaac tgctggatca ggtcggtgat cagcaccgtg cggctcgcgc
     3421 ggtggaaaaa caccacctcg gtggtgatca ggttgccgtg gaccaggaca tggtcgatgt
     3481 ccgccgacca gacatgaacc gagccgtcgg ccagatcgtc gtcgaaccgc aggtccggcc
     3541 gcttcccgcg caggccgggc ggcgcggtga accgtgcctc cagacaggcc tgccgccagc
     3601 tgtcgatatg caggtgatgc agggcgttcg gcgcgaccac atgacgaacc ggcccgagcg
     3661 cgtcgatctc ggccttgagg gcgtcatcaa gcgccgtcgg cgaccagacg aacagcccgc
     3721 ccccggccag ccgcatgacc gccattcgcg tcggatagcg gaacccggcc acatcggcga
     3781 cggggccatc ggcgatccac aggttatcgg cgaaagggcg cagcatgctg ttcccggaca
     3841 tcagggattc gcaggcatgc ccaggccggg ccggcttcca tggataggcc gccaagacaa
     3901 cgggcgcgga tcgctccgcg cccgtccatc attcgcaccg atcggggaca caggcctgcg
     3961 gtccatgtcc cttcaagatc acgcgctcgc gccgccggcc ttcttctcgg cgtagatcac
     4021 cagcggctgg gcgcggccct cgacgacctc gccgttgacc accacctcct cgacgccgtc
     4081 gtagctgggc agttcgaaca tcgtctccag caggatcgct tccatgatcg agcgcagtcc
     4141 acgcgccccg gtcttgcggg cgatcgcctt gcgggccacg gccgagaggg cgtcgtcggt
     4201 gaacttgagg ccgacgttct ccatctcgaa caggcgggcg tactgcttga tcagggcgtt
     4261 cttcggctcg gtgaggatct tgaccagggc ttcctcgtcg aggtcctcca gggtcgcgat
     4321 gaccggcaga cggccgatga actcggggat caggccgaag cgcagcatgt cctccggctc
     4381 gacctggcgc aggatttcgc cggtccggcg ctcgtccgga tcggccacct tggcgccgaa
     4441 gccgatcgag gtgcccgctc cgcggccgga aatgatcttc tccagtccag cgaaggcgcc
     4501 gccgacgatg aacaggatgt tggtcgtgtc gacctgcagg aactcctgct gcggatgctt
     4561 gcgcccgccc tgcggcggca cggaggcgac cgtgccctcc atgatcttca gcagcgcctg
     4621 ctgaacgccc tcgcccgaca cgtcgcgggt gatcgaggga ttgtccgact tgcggctgat
     4681 cttgtcgatt tcgtcgatgt agacgatgcc gcgctgggcc cgctcgacgt tgtagtccgc
     4741 cgactgcagc agcttgagca cgatgttctc gacgtcctcg ccgacatagc cggcttcggt
     4801 cagcgtcgtg gcgtcggcca tcgtgaacgg cacatcgatg attcgcgcca gggtctgggc
     4861 cagcagcgtc ttgcccgagc ccgtcgggcc cacgagcatg atgttcgact tggccagttc
     4921 gacgtcgttg ttcttctggg cgtggttcag gcgcttgtag tggttgtgca ccgcgacgga
     4981 gaggactttc ttggcgtgat cctgcccgat gacgtaatcg tccaggacct cccggatttc
     5041 cttgggagtc ggaacgccgt ccttggattt cacgaaggcg atcttgtgct cttcgcggat
     5101 gatatccatg caaagctcga cgcattcatc acagatgaac acggtcgggc cggcgatcag
     5161 cttgcgcacc tcatgctggc tctttccgca gaaagaacag tagagggtgc tcttcgagtc
     5221 gccgctggcg gcctttgtca ttgctcttct cacgctctga cgacgaaggc ccaagcccgg
     5281 cgccgcccac ccctatgtgg ctcgtcctgc ctctgaacat gcaggatacg cgccgcgggt
     5341 tttccaatcc ttgacaatcc ccgatccggc gttcggggac aagcgccctg acgccacagg
     5401 gcgttcggaa acgcccccgc gtcggcacgt cgattgcagt ctcgctctcg gcaacagatg
     5461 ggaattgacc cgctgtggga caagcgatcg agcgccgccg acctcccgcc gacgggcggg
     5521 acgatgtgac gccggtgtga catgtcccgg aatcgtgatc gaaacggccc tcgagaaggc
     5581 cggggttggg gaaacatggg acgcagatcg ctactggcgc aggggctcag cgcctcccgg
     5641 ccagtgatgc ctgacccgct cgaggcgccg gccctggtgg ccttcgactt cgacggcacc
     5701 ctgacggtcg aggacagctt caccgccttc ctgaaatggc gcgcggggcc gttgcgctgg
     5761 gccttcggct gcctgcgact gacgcctgcc gcgatcggct acctgttcca tcgcaaccgc
     5821 ggccgcatga agtccgcggc catccgcgaa tttctcaagg gaacgccgcg ggagaagctc
     5881 gaggcccagg cccgcacctt cgccgagctg gccgcgccgc gcctgtttcg tcccgacgcc
     5941 ctgatgacct ggcgtcgctg gcgggccaac ggcgcgcgca ccatcatcgt cagcgcctcg
     6001 cccgacctga tcgtggcgcc gttcgcccgg ggcctgggct gcgaccggct gatcgccagc
     6061 gccatcgcgt tcgacaggga tgaccgcgtg atcggcatcc tggccggccc gaattgccgc
     6121 ggccccgaga aggtccgccg cctgcgcgag gcctacggcg aggacgtcca tctgaccgcc
     6181 gcctatggcg acacgtccgg tgatcgcgag atgctgcgga tcgccgacga aaagggctat
     6241 cgggtgttca agggccggcc ctgatttcct gctgacagcc tgcggcatga gtgctgccat
     6301 gctgccggtc cggagatcgc gccatgaaac acgccggccc cgccgccctc gatcagcttg
     6361 aaccgctgct ggcggctatc cgcgccctgc ccggcctgaa ggagaagacc cgcggcgtgt
     6421 tctaccgcgg cggacgggcc ttcctgcact tccacgagga ctccgccggc ctgttcgccg
     6481 acgtgcggcc ggccgggccg gacttcgagc gcttccgggt ggagaccgag gcggagcaag
     6541 gcgccttcgt cgccttggtc accgtcaaac tcaccgcgta gcggatacaa aaaaggcccg
     6601 gcgcgagccg ggccttctct gttcgggacc gaatgtccta ggacttttcg cccgccgcgc
     6661 cctcggccgc ctcgcggctt tcgtagacgt ggtcgacgat gccccaggcc ttggcgtcct
     6721 cggcgctcat gaagtggtcg cggtcgaggg tcgtctcgac ctcgtcatag gtgcggccgg
     6781 tgtgcttcac atagatctcg ttgaggcgct tcttggtcgc caggatgtcc tcggcgtggc
     6841 gcatgatgtc gctggcctgg ccgcggaagc cgccggaggg ctggtggacc atgatgcggg
     6901 cgttgggcag ggcgatgcgc tggccggcgg cgccggcgca cagcagcagg ctgcccatcg
     6961 aggcggccat gcccatgcag accgtcgaca ccgggctctt gatgtattgc atggtgtcat
     7021 agatcgccag gccgctggtc accacgccgc ccggcgagtt gatgtacatg ctgatctcct
     7081 tcttcgggtt ctccgactcc aggaacagca gctggctgca gatcagggcg ctcatgccgt
     7141 cctcgaccgg accggtgagg aagatgatcc gctccttcag cagccgcgag aagatgtcga
     7201 acgcccgctc gccgcggctg gtctgttcga ccaccatggg cacgagattg agggcggtct
     7261 ggacagggtc cgacgacgaa atggtccggt agtaatcgct catgggcgcc ttcctctatg
     7321 cactggcccg tcgcgaatca tgccgcgcca tcgcctcccc ccggatatcg ggcggaggtt
     7381 aagaggatgc aagtgggcgc gacccgacgc tgcggcgcaa gtcggacggg atcgtggcgt
     7441 tcaggggccg gcgggcggcg cgggggcgct cgcggccttg gggccgagcc ggcgcagcat
     7501 gtcctgggcg atctcgcgtt cctggcggct gccgcggacg acgtcgcggt tggcgccata
     7561 gaggcccagc agataggcgg cgtcccattc ggtcaggccg tcggccggtt cggcgccccg
     7621 gtcccgggcg gcgaatagcc ccaggatcga ctgggtcttg cccaggtcga tgtcaggttc
     7681 gatctgcgcc agaccgatca tggtgatgta gtcggccagg gcctcgaagc ggatcacgcc
     7741 gatccgtcgc acatcgacca ggatgatcac gtggtcgaag tcgtcgcggg tggtcgcccg
     7801 caaccggctg gccgagcgga cacgcacgcc gctgccctgg gtgtagctct cgccctcggc
     7861 cgtcttggtg cgggtcacgt gccaccagcg caccggcgcg ggggtttcca cgaaggcctt
     7921 cagcgcccgg tcgccgcggc tcatgccccg ctcccggcgg gcgaaggcgg ccggccgtga
     7981 atccacgacc gaccgggcca gcgcctggga atcgtcggtg g
//
LOCUS       DAORNX010000084         7985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6669_length_7985_cov_7.030643, whole genome shotgun sequence.
ACCESSION   DAORNX010000084 DAORNX010000000
VERSION     DAORNX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6669_length_7985_cov_7.030643"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /locus_tag="PLO65_05380"
     CDS             <1..2266
                     /locus_tag="PLO65_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27714.1"
                     /translation="GLSLSPALALAAEGEAAVLTADADAAPIDLQGLDVYGEKADRPS
                     SPKYTAAPVDTPQTVTVVSERTVRDQNLLTLRDILQTVPGITFGAGEGGGGYGDSINL
                     RGYSANNDVTTDGLRDSAQYSRTDPFNLQQIEVINGANSVYAGAGSVGGSINLVSKTP
                     RMSDSGLLSGGVGTDGYGRLTLDANRVLGEVAAVRLNLMVHGNDAPGRDVERFSRWGI
                     APSITLGLTGPTQVTLSGFHQEDDNVPQYGVPYASNAFLNGPLPGVDPSSYYGYRNID
                     TQEIGVDSLTLRIVHDFSESLSVTSTARWQQVTQRTIVDPPQGTWCLASGFNAQTGAA
                     CATPGVYILSGPRGNLRDTTNSQLVSQTDFNMKFATGGWSHNLVLGVSFSNETYDLTT
                     GRLFFNDDGSAVVLPTMDIADPDNRYAGPVNFHTSAISDGELANQAIYVFDTVELNSH
                     WQFNGGLRVERNTGEFVSTPYTWAVGTPPTRIAGATTVAKNEETLVSWRVGLVYKPAP
                     NASLYIALGNTRTPSQATVNGGCSTTGANQNCNVDPEDGEVLEVGAKWDALDGRLSLT
                     AALFQNERSNIRLNSNDPSIPVQQLDGASRVRGATFGVAGQLTDRWTILANYTWLDSE
                     ILQNIASTALPPNDVDYTRGDPIPMTPEHAFNLWTTFMVTDTVMVGYGATYAGDYAFT
                     RANAAAPLLFSDAYWLHNAAVTWTVNDGVSVQLNVKNLTDEEYYTRVRSNSGFGWATP
                     GDARSAQVTLNYRF"
     gene            2294..2977
                     /locus_tag="PLO65_05385"
     CDS             2294..2977
                     /locus_tag="PLO65_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HRD27715.1"
                     /translation="MMLQIPGVLTREEVGRLRALIDRAEWIDGNVTAGFQSALAKQNR
                     QLAEGSPAAREAGATILAALERNPLFIAAALPARIYPPLFNRYGVGDGFGDHIDNSIR
                     RNRLDGAQVRTDLSATLFLTEAEDYDGGELTVDDTYGVHQVKLAAGDLILYPASSLHR
                     VEAITRGARVSSFFWIQSLVRDDARRALLFDMDMAIQRAGTTLGQGDPSIVSLTGAYH
                     NLLRMWAEP"
     gene            3036..5384
                     /locus_tag="PLO65_05390"
     CDS             3036..5384
                     /locus_tag="PLO65_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRD27716.1"
                     /translation="MAGGKTGNRSTRLRRAETAAARWFFDNALDMFAVVNREGVFTEI
                     NDAWERVTGWRREDLVGRTIFEFLHEDSYEEARDSRRRMKRDGQTVNQLRIRRKNGEW
                     MWIQGRSRLDPNDDMVGTLHDITEMVLQKEELEAARKTRSMLSEAAGIGTWSYEPHGD
                     RIEWSEDILALCGWDQADIDTTAKFHAIVDPVQKEMVNAVFARGVTTGQGATIEHRFL
                     TRDGRWLTMRATFRTERRGKVYALKGISQDVSELAEARDAAREGERRVQALMEQARMD
                     AWRQELALGAADAGAFEIDHAGKGFWASEQFFRLVGRPMTYDEIAAPVWSFVHRDDRG
                     LVFETNRRWARGEENISMEFRILLPDGQERWVRAFYRLDRPTRKGVGLVLDIDARKRQ
                     ELALVEAQRAAEAAAEAKASFLANMSHEIRTPMNGVLGVLHLLKGEEVSEAGRRLLDE
                     ALSCGEMLSTLLDDVIDFERIEAGRLELNDEPVDAGELARSVFRLLEPQATAKGLTLV
                     LEGDMDLGWVRSDPVRLRQCLFNLLGNAVKFTLKGGVTLRCARPAPDRLAFAVQDTGI
                     GISREAQGRLFERFHQADASTTRRFGGSGLGLTITRRLAEMMGGQVSFQSTPGLGSTF
                     RIEIAAPEAEAAGPVADPAGSPLEGLRLLVVEDNATNRMIAVRMLESLGASAQTAEDG
                     ASGVAAAAAGVFDLILMDIQMPDMDGLEACRRIRAQAGPSAMTPIIALTANVLSHQRD
                     TYLAAGMDGVVGKPISPAALLAEISRLAEDPTDAAPAESRVA"
     gene            5505..>6891
                     /locus_tag="PLO65_05395"
     CDS             5505..>6891
                     /locus_tag="PLO65_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD27717.1"
                     /translation="MASSAGTAFEWYDFFVFGTLASVIGKNFFAELGDTAGVLAALGL
                     FGAGFFFRPLGALIFGRIGDKAGRKGAFLITVILMGGATFAIGLLPTYATAGPLAPML
                     LILMRIIQGTALGGEYGGAAIYVAEHAPPGRRGAMTGWIQTSAAFGLLGALLVILAAR
                     TALGEEAFLAWGWRIPFLVSVGLLGVSIWMRLKLSESPSFQKLKDAGEESKHPFREAF
                     GQWKNLKLVLLALVALMFAQGAVWYTAFFYLPVFLERFLKVEPRTVNLLVMGATALSA
                     PLYVFFGWLSDKVGRKWVLWFGMTLALVAFFPAFHQMARYANPALAEAEQRTPVSVVA
                     DPAACSLQFDPVGKAVFTTSCDIAKSALANAGVSYVNTPAPAGTPATVRVGAVEVASV
                     EGAGLDKAALKAAKADFETRLKAALGAAGYPAGAERGRMNLPALFGVIMVFVVAATAL
                     YGPQASALVELF"
     assembly_gap    6892..6950
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6951..7104
                     /locus_tag="PLO65_05400"
     CDS             <6951..7104
                     /locus_tag="PLO65_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27718.1"
                     /translation="GGFVPFTAFAIVTATGDIYAGLWYPFVFTLISVVATLLLWPETR
                     GRDLDA"
     gene            complement(7101..7793)
                     /gene="modB"
                     /locus_tag="PLO65_05405"
     CDS             complement(7101..7793)
                     /gene="modB"
                     /locus_tag="PLO65_05405"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRD27719.1"
                     /translation="MTGLTPADLAAIGLSLKVAATAMLLSLPVAFSVALLLARSRFPG
                     RSLLDAAVHLPLVLPPVATGYVLLILLGRNGPLGRPLAEIGVVFAFDWTGAALAAAVM
                     AFPLMVRAIRLAIEAIDPEVEEVARTLGARPLLRLLRVTLPLAAPGLIAGAVLGFAKA
                     LGEFGATITFAAAIPGATLTLPTAIYQAVQQPGSEGRALTLCAVATVIAVAAVIAANA
                     FGRAVERPTGRG"
     gene            7874..7985
                     /locus_tag="PLO65_05410"
                     /pseudo
     CDS             7874..7985
                     /locus_tag="PLO65_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1238 a         2859 c         2696 g         1133 t
ORIGIN      
        1 cggattgtcg ctgtcaccgg ccctggccct ggccgccgag ggcgaggccg ccgtcctgac
       61 cgcggacgcc gacgccgcgc cgatcgacct gcagggtctc gacgtctatg gcgaaaaggc
      121 cgaccgcccg agctccccga agtacaccgc cgctccggtc gatacgccgc agaccgtgac
      181 ggtggtctcc gaacggaccg tgcgcgacca gaacctgctg accctgcgcg acatcctgca
      241 aacggtgccc ggcatcacct tcggggccgg cgagggcggc ggcggctatg gcgacagcat
      301 caacctgcgc ggctattcgg ccaacaacga cgtgaccacc gacggcctgc gcgacagcgc
      361 ccagtacagc cgaaccgacc ctttcaatct gcagcagatc gaggtgatca acggggccaa
      421 ctcggtctac gccggggccg gttcggtcgg cggctcgatc aatctcgtca gcaagacgcc
      481 gcgcatgtcc gacagcggcc tgctgtcggg gggcgtcggc acggacggct acggccggct
      541 gaccctcgac gccaaccggg tgctgggcga ggtcgcggcc gtgcggctca acctgatggt
      601 ccatggcaat gacgccccgg ggcgggacgt cgagcggttc agccgctggg ggatcgcccc
      661 gtcgatcacc ctgggcctga ccgggccgac ccaggtcacc ctctcgggct tccaccagga
      721 agacgacaac gtcccgcagt acggcgtgcc ctacgcctcg aacgccttcc tcaacggacc
      781 gctgccgggc gtcgatccgt cgagctacta tggctaccgc aacatcgata cccaggagat
      841 cggcgtcgac agcctgacac tgcggatcgt gcacgacttc agcgagagcc tgtcggtgac
      901 cagcacggcc cgctggcagc aggtcaccca gcggaccatc gtcgacccgc cgcaggggac
      961 ctggtgcctg gccagcggtt tcaacgccca gaccggcgcg gcctgcgcga cgcccggcgt
     1021 ctacatcctg agcggcccgc gcgggaacct gcgcgacacc accaacagcc agctggtcag
     1081 ccagaccgac ttcaacatga agttcgccac cggcggctgg tcgcacaatc tggtgctggg
     1141 cgtgtccttc tcgaacgaga cctatgacct gaccaccggc cggctgttct tcaacgacga
     1201 cggctcggcg gtggtcctgc cgaccatgga catcgccgac cccgacaatc gctacgccgg
     1261 cccggtcaac ttccacacca gcgcgatcag cgacggcgag ctggccaacc aggcgatcta
     1321 tgtcttcgat acggtcgagc tgaacagcca ctggcagttc aacggcggcc tgcgggtcga
     1381 gcgcaacacg ggcgagttcg tgtccacccc ctacacctgg gccgtcggaa cgccgccgac
     1441 gcggatcgcc ggcgcgacca ccgtcgcgaa gaacgaggag acgctggtct cctggcgggt
     1501 cgggctggtc tacaagcccg cgccgaacgc cagcctctac atcgccctgg gcaatacccg
     1561 cacgccttcc caggcgacgg tcaacggcgg ctgttcgacc accggcgcca accagaactg
     1621 caatgtcgac cccgaggacg gcgaggtgct ggaggtcggc gccaaatggg acgccctgga
     1681 cggacgcctg tcgctgaccg ccgccctgtt ccagaacgag cggagcaaca tccgcctgaa
     1741 ctcaaacgac ccgtcgatcc cggtgcagca gctcgacggc gcatcgcgcg tccggggcgc
     1801 gaccttcggc gtggccggtc agctgaccga ccgctggacc atcctggcca actacacctg
     1861 gctggacagc gagatcctgc agaacatcgc ctcgaccgcc ctgccgccca acgatgtcga
     1921 ctacacccgc ggcgacccga tcccgatgac gccggaacat gcgttcaacc tgtggaccac
     1981 cttcatggtc accgacacgg tcatggtcgg ctacggcgcg acctacgccg gcgactacgc
     2041 cttcacccgc gccaatgcgg ccgcccccct gctgttctcc gacgcctact ggctgcacaa
     2101 tgcggctgtg acctggacgg tgaacgacgg cgtcagcgtc cagctcaacg tgaagaacct
     2161 gaccgacgag gaatactata cccgcgtccg gtccaattcc ggcttcggct gggccacgcc
     2221 gggcgacgcc cggtcggccc aggtgacgct caactaccgc ttctaggggc cagccggccg
     2281 aggacgaccc gccatgatgc tgcagattcc cggcgtgctg acccgggagg aggtcggacg
     2341 gctgcgcgcc ctgatcgacc gggccgagtg gatcgacggc aatgttaccg ccggcttcca
     2401 gtccgcgctg gccaagcaga accggcagct ggccgaaggc tcgccggccg cgcgcgaggc
     2461 cggcgcgacc atcctggcgg cgctggagcg caatccgctg ttcatcgccg cggccctgcc
     2521 cgcgcgtatc tacccgccgc tgttcaaccg ctacggcgtc ggcgacgggt tcggcgacca
     2581 catcgacaat tccatccgtc gcaaccggct ggacggcgcg caggtgcgca ccgacctgtc
     2641 ggcgaccctg ttcctgaccg aggccgaaga ctacgacggg ggcgagctga cagtcgacga
     2701 cacctatggc gtccaccagg tcaagctcgc ggcgggcgac ctgatcctct atccggcctc
     2761 cagcctgcat cgggtcgagg cgatcacccg cggcgcacgg gtgtcgtcgt tcttctggat
     2821 ccagagcctg gtgcgcgacg acgcccggcg ggccctgctg ttcgacatgg acatggctat
     2881 ccagcgcgcc ggcacgaccc tgggccaggg cgatccctcg atcgtctcgc tgaccggcgc
     2941 ctaccacaac ctgctgcgca tgtgggccga gccttgagaa cctaacgttt acgggtcgcg
     3001 gccaatggtc ggaccgtaac gggcggaatc aaggcatggc cggcggcaag accggaaatc
     3061 gatcgaccag gctgcgccgc gctgaaaccg ccgccgcgcg atggttcttc gacaacgccc
     3121 tggacatgtt cgcggtcgtc aatcgcgagg gcgtgttcac cgagatcaac gacgcctggg
     3181 agcgggtcac cggctggcgg cgcgaggatc tggtcggcag gaccatcttc gaattcctgc
     3241 acgaggacag ctacgaggag gcgcgcgact cgcgccggcg catgaagcgc gacggccaga
     3301 ccgtcaacca gctgaggatc cgccgcaaga acggcgagtg gatgtggatc cagggccgct
     3361 cgcggctcga ccccaacgac gacatggtcg gcacgctgca cgacatcacc gagatggtgc
     3421 tgcagaagga ggagctggag gcggcccgca agacccgatc gatgctctcc gaggccgccg
     3481 gcatcggaac ctggagctac gagccgcacg gcgaccgcat cgaatggtcc gaggacatcc
     3541 tggccctctg cggctgggac caggccgaca tcgacaccac cgccaagttc cacgccatcg
     3601 tcgatccggt ccagaaggag atggtcaacg ccgtcttcgc ccgcggggtg acgaccggcc
     3661 agggcgcgac gatcgaacat cgtttcctga cccgcgacgg gcgctggctg accatgcgcg
     3721 cgaccttccg caccgagcgg cgcggcaagg tctacgccct gaagggcatt tcccaggacg
     3781 tcagcgagct ggccgaggcc cgcgacgcgg cccgcgaggg cgagcggcgg gtccaggccc
     3841 tgatggagca ggcgcggatg gacgcctggc gccaggagct cgcgctcggc gccgccgacg
     3901 ccggcgcctt cgagatcgac cacgcgggca agggcttctg ggccagcgag cagttcttcc
     3961 ggctggtcgg ccgtccgatg acctacgacg agatcgccgc cccggtgtgg tccttcgtgc
     4021 atcgcgacga ccgcggcctg gtgttcgaga ccaaccgtcg ctgggcgcgg ggcgaagaga
     4081 atatctcgat ggaattccgc atcctcctgc ccgacgggca ggagcgctgg gtgcgggcct
     4141 tctatcgact cgaccggccg acccgcaagg gcgtgggcct ggtgctcgac atcgacgccc
     4201 gcaagcgcca ggagctggcg ctggtcgagg cccagcgggc cgccgaggcc gccgccgagg
     4261 ccaaggccag cttcctcgcc aacatgagcc acgaaatccg cacgccgatg aacggcgtgc
     4321 tgggcgtgct gcatctgctc aagggcgagg aggtcagcga ggccggccgg cggctgctcg
     4381 acgaggcgct cagctgcggc gagatgctct cgaccctgct ggacgacgtg atcgatttcg
     4441 agcgcatcga ggccggtcgg ctcgagctga acgatgaacc ggtcgacgcc ggcgaactgg
     4501 cccgcagcgt gttccggctg ctggagccac aggcgacggc caagggcctg accctggtcc
     4561 tcgagggcga catggacctg ggctgggtcc gatccgatcc ggtgcggctg cggcaatgcc
     4621 tgttcaacct gctcggcaat gcggtgaagt tcaccctgaa gggcggggtg actctgcgct
     4681 gcgcccgccc tgcccccgat cgcctggcct tcgccgtcca ggacaccggc atcggcatct
     4741 cgcgggaagc ccaggggcgc ctgttcgagc ggttccacca ggccgacgcc tcgaccaccc
     4801 gccggttcgg cggctcgggc ctgggtctga cgatcacccg ccggctggcc gagatgatgg
     4861 gtggccaggt gtccttccag tcgacgcccg gcctcggctc caccttccgt atcgagatcg
     4921 ccgccccgga ggccgaggcc gccggtccgg tcgccgatcc ggccggatcg ccgctggaag
     4981 gcctgcggct gctggtggtc gaggacaacg ccaccaaccg gatgatcgcg gtcaggatgc
     5041 tcgagagcct cggcgcctcg gcccagacgg cggaggacgg ggcctcgggc gtcgccgccg
     5101 ccgccgcggg ggtgttcgac ctgatcctga tggacatcca gatgcccgac atggacgggc
     5161 tggaggcctg ccgacggatt cgcgcccagg ccggaccctc ggccatgacg ccgatcatcg
     5221 ccctgaccgc caatgtcctg tcgcaccagc gcgacaccta tctcgccgcc ggcatggacg
     5281 gcgtggtcgg caagccgatc tctcccgccg ccctgctggc cgagatctcc cgcctcgccg
     5341 aagaccccac cgacgcggcc cccgcggaaa gccgggtggc ctgaacatct tccgccgccg
     5401 cgcggttctg ctagaccttg cgccgtgacg tctgggggaa ctgcaggatg acgacggcga
     5461 cagcggcgcg gcaggatgcg cccgcctcga tgcgaacggt ggtcatggcc tcgtccgcgg
     5521 gtacggcctt cgagtggtat gacttcttcg tcttcggcac cctggcctcg gtcatcggca
     5581 agaacttctt cgccgagctc ggcgacaccg caggcgtgct ggcggccctg ggcctgttcg
     5641 gggccggctt cttcttccgg ccgctcgggg cgttgatctt cggccgcatc ggcgacaagg
     5701 ccggccgcaa gggcgccttc ctgatcaccg tcatcctgat gggcggcgcg accttcgcca
     5761 tcgggctcct gccgacctac gccaccgccg gaccgctcgc gccgatgctg ctgatcctga
     5821 tgcggatcat ccagggcacg gcgctgggcg gggagtacgg cggggcggcg atctacgtcg
     5881 ccgaacacgc gccgccgggc agacgcgggg cgatgaccgg ctggatccag acgtcggccg
     5941 ccttcggcct gctcggagcc ttgctggtga tcctggcggc ccgcacggcg ctgggcgagg
     6001 aggcgttcct ggcctggggc tggcgcatcc cgttcctcgt gtcggtcggt ctgctcgggg
     6061 tgtcgatctg gatgcggctg aagctgtcgg aaagcccgtc attccagaag ctcaaggacg
     6121 ccggcgagga gtcgaagcat ccgttccgcg aggccttcgg ccagtggaaa aacctcaagc
     6181 tggtgctgct ggcgctggtg gcgctgatgt tcgcccaggg cgccgtctgg tacactgcct
     6241 tcttctacct gccggtgttc ctcgagcggt tcctcaaggt cgagccgcgc accgtcaacc
     6301 tgctggtgat gggcgccacg gccctcagcg cgccgctcta tgtgttcttc ggctggctgt
     6361 ccgacaaggt cgggcgcaag tgggtgctgt ggttcggcat gaccctggcc ctggtcgcct
     6421 tcttcccggc cttccaccag atggcgcgct acgccaaccc ggccctcgcc gaggccgagc
     6481 agcgcacgcc ggtctcggtg gtcgccgacc cggccgcctg cagcctgcag ttcgaccccg
     6541 tcggcaaggc ggtgttcacc acctcctgcg acatcgccaa gagcgccctg gccaacgccg
     6601 gggtgtccta cgtcaacacg ccggcgccgg cgggaacccc ggccacggtc agggtcggcg
     6661 cggtcgaggt cgcctcggtc gagggcgccg gcctggacaa ggcggcgctc aaggcggcca
     6721 aggccgactt cgaaacccgc ctcaaggcgg cgctcggcgc ggcgggctat ccggccgggg
     6781 cggagcgcgg gcggatgaac ctgccggccc tgttcggggt gatcatggtc ttcgtggtgg
     6841 cggccaccgc cctttacggg ccccaggcgt cggctctggt cgagctgttc cnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggcggcttc
     6961 gtgccgttca ccgccttcgc catcgtcacc gccaccggcg atatctacgc cggcctctgg
     7021 taccccttcg tcttcaccct gatcagcgtg gtcgcgaccc tcctcctgtg gccggagacc
     7081 cgcggccggg acctggacgc ctagccccgc cccgtcggcc gctcgacggc ccggccgaag
     7141 gcgttggcgg cgatcaccgc ggccacggcg atcaccgtcg ccacggcgca gagcgtcagg
     7201 gcccggccct cgctccccgg ctgctgcacc gcctggtaga tggccgtcgg cagcgtcagg
     7261 gtggctcccg ggatcgccgc ggcgaaggtg atggtcgcgc cgaactcgcc gagcgccttg
     7321 gcgaagccca gcaccgctcc cgcgatcagg ccgggcgcgg ccagcgggag ggtgaccctc
     7381 agcaggcgga gcaggggccg ggcgccgagg gtccgggcga cctcctcgac ctccgggtca
     7441 atcgcctcga tcgccagccg gatggcgcgg accatcagcg gaaaggccat caccgccgcg
     7501 gccagcgccg cgccggtcca gtcgaaggcg aacaccaccc cgatctccgc cagcggccgc
     7561 cccagcggtc cgttgcgccc gagcaggatc agcaggacat agccggtggc cacgggcggc
     7621 agcaccagag gcaggtgcac cgccgcgtcc agcagcgatc ggccaggaaa ccggctgcgg
     7681 gccagcagca gggcgaccga gaaggccacc ggcagggaca gcagcatggc cgtcgccgcg
     7741 accttgagcg acaggccgat ggcggccagg tcggccggcg tcaggccggt catgcgtcgg
     7801 gcgcggcgag caggacctcg accgccaccc cggcttcggc gagcgcgcgg acctcgcgcg
     7861 ggtcgatcgg cccggtgacc agccggccgc gatcgaacgg ctgaccgcgg gattcgaggc
     7921 cgggctggcc caccagaccc tgctcggcgt caccggctcg ggcaagacct acacgatcgc
     7981 caacg
//
LOCUS       DAORNX010000085         7966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6691_length_7966_cov_7.317280, whole genome shotgun sequence.
ACCESSION   DAORNX010000085 DAORNX010000000
VERSION     DAORNX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6691_length_7966_cov_7.317280"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1565
                     /locus_tag="PLO65_05415"
     CDS             126..1565
                     /locus_tag="PLO65_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRD27720.1"
                     /translation="MSRFVQGADRQQPTLLPECLDDYVTEDNPVRVVDVFVDELDLKS
                     LGFEGMTPAATGRPAYHPAMLLKLYVYGYLNKVQSSRRLEREAQRNVELMWLTGRLAP
                     DHKTIADFRRENGSAIQAACAQFVVLCRQLGLFGAALVAIDGSKFKAVNTRDRNFTAY
                     KVKRRIEQVAEHIAGYLQDLDTADRHEGEAAEARSGRLVEKIDRLRAQMAMLKTMEAE
                     VEASPDGQVSLTDPDARSMATSGRGSGIVGYNVQSAVEGEHHLIVAHEVVMTGSDREQ
                     LASMASKARDAMGVEALDVLADRGYFSGEEILACETMGVTPFVPKPLTSGAKAVGRFG
                     KQDFVYLPDQDVYRCPAGALLPRHMTTVEKGMTLHRYWDRASCTGCRLKPQCTPSIER
                     RVTRWEHEAVIDALQARMDLAPRAMRTRRRLVEHPFGTIKAWMGHTHFLMKRLPNVKT
                     EISLHILAYNIKRVMAVLGTKPLMEAMRA"
     gene            complement(1707..2177)
                     /locus_tag="PLO65_05420"
     CDS             complement(1707..2177)
                     /locus_tag="PLO65_05420"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRD27721.1"
                     /translation="MGGSGGGGGYSRLGDVKGLEQKAKEALQGGRKNVFLSFSMKDID
                     EVNLVRAHAKNENSDIEFNDRSVREPYDSERAEYIRSRLKERINQSSTTVVYVSENTA
                     KSDWVKWEVETSLKLGKRVIAMYKGDQPPSNTPAWMKDPNITSVAWKNLAKELS"
     gene            complement(2177..2770)
                     /locus_tag="PLO65_05425"
     CDS             complement(2177..2770)
                     /locus_tag="PLO65_05425"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HRD27722.1"
                     /translation="MSVVTLVSGGLDSLLVACLAKEEGLTQFPLFIDYGQRALAQEYA
                     ACVKGMARMGLPPPVSASLSGYGQLIRSGLTDRGQRVYEDAYTPGRNLLFLLTGAAYA
                     AQVGADAVSIGLLHEDTSLFPDQTSDFLRSAEATLALAVAKPVRVLAPLAAFHKRDVV
                     QLAKEKGLAGSYSCHLGEAEPCGRCIACREFLFEEAG"
     gene            complement(2767..3480)
                     /locus_tag="PLO65_05430"
     CDS             complement(2767..3480)
                     /locus_tag="PLO65_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27723.1"
                     /translation="MIARHSPERICHAVSVVGAEIDRQLSGETPEPWSEDRLWTELAC
                     CVLSSQVPYSIAQLAAVRVEASGLLLEGHTSAEGLEGALAELLKRPFEIQGTLRRYRF
                     PDSRARQLAATAMKVRCHADGLAGLLAGFDEIESARDWFVTHAPGLGPKQASMFLRNV
                     GASYDLAVLDRHVINYMTIVGLTADAAPTRRMVDYRRDEIILRDHAAGFGLPVGFLDW
                     AVWIVMRVASSKVSKERLQ"
     gene            complement(3477..4886)
                     /locus_tag="PLO65_05435"
     CDS             complement(3477..4886)
                     /locus_tag="PLO65_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRD27724.1"
                     /translation="MADICIIYARSDASAIPPALEAVLKTRWSVWWDRHLVSGDYRAG
                     IEAEVTACEVLIPIWSTAATFNAHMHDELEIARKAGVAILPVRIHAADAPMSYGSLQA
                     TEMLGWDGKETLPQLNSLMGRVRAAIEERRPDERPQRLAMNGFVDLPAFFYSVSSHET
                     RLAPAAALRTLDMFGAETILVSAYDTGPNAPKSLIQTLERRRKRGATILLDSGNYERA
                     RRDDGDWKFETFETALRATPHDLAFAFDVLNPTGDIDKVAKAAIAAAKRTARLTTAPV
                     IPIVHLPRRPDDSYRTEMAGPVMRHVAQALAPAMIAIPERELGSGVFERARTMSIIRK
                     ALKGLGRYQPVHVLGAGNPISLALLSAAGADCFDGLEWCRYAFDAEHAQLHHFQLFEL
                     FSYQLAVAESPVAASAALDPAVSYTTKAVMHNLDFYNTWLKELRSAAQEEKALVAFLA
                     GLLPKGAIAQARAALPSVL"
     gene            complement(4968..6092)
                     /locus_tag="PLO65_05440"
     CDS             complement(4968..6092)
                     /locus_tag="PLO65_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRD27725.1"
                     /translation="MNAIIVAGGCYVETCEAPHWRAVYGSGGRAAHALVGQAPVILHT
                     YFPKARENDLYPLAVAGIEVKATDAASAIAFAYFHPLSDPAMAPERASITRQPALRVE
                     GEVVLRFGFVEGDAVVSGRRVVYDPQSVGDFAPYSQNGSSAETLALVLNADELRAATR
                     QDDLSVGARALLDAGAAVVLVKCGVHGAVVYAGDGVGTQIPAFWSDAVFKLGTGDVFS
                     AAFTHAWAHLRQDAAAAATVASRSVAHYAATRALPLPPAGTMHPGRAVTASPSAKVHL
                     IGSKTQLANRWLFEEASWRLGQLGADVRRWDGPPESQFNDGACLVLADQIGADDLAEI
                     AARSDPNRLILLSETGVSPPAHARFTTDFTTALYWACWAG"
     gene            complement(6108..7025)
                     /locus_tag="PLO65_05445"
     CDS             complement(6108..7025)
                     /locus_tag="PLO65_05445"
                     /inference="COORDINATES: protein motif:HMM:NF033610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HRD27726.1"
                     /translation="MGANFVYVRILGVAMEFPALYAVADRAAGRQQSLYFGLVFGEFT
                     ALLMVSCMSVNMVADSRYVFAYGVLLAGALTLLVLRTVLKPEQRWYQARALAESVKTT
                     AWKFAMGARPFKQDGEHRERLRRMLQELLDANRSLGSQFDGKTSAGDQITKDMEAIRA
                     LPHAERLTIYIRDRIDNQLGWYGRKSKINADTGRNFAIVLGGLYVAAFAIFMIRMEHP
                     DWIYAHPDPVILLGASVLGWMQIKRFNELGASYALTANEIGILKSRAPEIADDEALSL
                     FVDDAEEAFSREHTQWLARRSALGGDRGG"
     gene            complement(7205..7669)
                     /locus_tag="PLO65_05450"
     CDS             complement(7205..7669)
                     /locus_tag="PLO65_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRD27727.1"
                     /translation="MRTIDFSPLYRSVVGFDRLAAQLDAAATQDSAAGYPPYNIERTD
                     ENAYRIEIAVAGFRPDELTIEVKENLLTVTGRKAANDDSRKFLHRGLAERNFERRFQL
                     ADYVLVTDANLADGLLSIALRRELPEALKPRTIAIGTTTTAAPAQIKGKKAA"
BASE COUNT         1483 a         2520 c         2492 g         1471 t
ORIGIN      
        1 ccatggcgtg cgggaggtct acttcccttc ccgatgtgac tttccgcttt cgaccgaggc
       61 tgtgtgaaaa cgcggctgga tcgagcaaat cagggtagcc ttctggggtc agcaggaggt
      121 gggtcatgag ccgcttcgtt cagggtgcag accgccaaca gcccacgctg cttcccgaat
      181 gtctcgacga ctatgtgacc gaggataatc cagttcgggt ggtcgatgtg ttcgtcgacg
      241 agctggatct gaagagcctg ggctttgagg gtatgacgcc ggcggcgacg ggccggccgg
      301 cctatcaccc ggccatgctg ctgaagctct acgtctacgg ctatctgaac aaggtgcagt
      361 cgagccggcg gctggagcgg gaggcgcagc gcaacgtcga gctgatgtgg ctgacgggac
      421 ggctggcgcc ggaccacaag accatcgccg acttccgccg cgagaacggt tcggcgatcc
      481 aggcggcgtg cgcccagttc gttgtgctct gccgccagct tggcctgttc ggcgcggccc
      541 tggtcgccat cgatggctcc aagttcaagg cggtgaacac ccgcgaccgc aacttcaccg
      601 cctacaaggt gaagcggcgg atcgaacagg ttgccgagca catcgccggc tatctgcagg
      661 acctcgacac ggccgatcgc cacgagggcg aggcggccga ggcgcggtcc gggcgactgg
      721 tcgagaagat cgatcggctc cgcgcacaga tggcgatgct gaagaccatg gaggcggagg
      781 ttgaggcgtc gcctgacggc caggtctccc tgaccgaccc cgacgcccgc tccatggcga
      841 cgtcgggccg gggcagcggc atcgtcggct acaacgtcca gtccgccgtc gaaggcgagc
      901 atcacctgat cgtcgcccac gaggtggtta tgaccggctc agacagggag caactggcct
      961 cgatggcgag caaggccagg gacgctatgg gcgtggaggc gctcgatgtg ctggccgacc
     1021 gcggctactt ctccggagag gagatcctcg cctgcgaaac catgggcgtg acgcccttcg
     1081 tgcccaagcc cctgacctca ggcgccaagg cggtgggtcg cttcggcaag caggacttcg
     1141 tctatctgcc cgatcaggac gtctatcgct gcccggccgg cgcgctgctg ccgcgccaca
     1201 tgacgaccgt cgagaagggc atgaccctgc accgctactg ggaccgggcc agttgcaccg
     1261 gctgccgcct gaagccgcag tgcaccccca gcatcgagcg tcgggtcacc cgctgggagc
     1321 atgaggcggt gatcgatgcg ctgcaggccc ggatggacct ggccccgcgc gccatgcgca
     1381 cccgacgacg gctggttgaa cacccgttcg gcacgatcaa ggcctggatg ggccacaccc
     1441 acttcctgat gaagcggttg cccaacgtga agaccgagat cagcctccac atcctggcct
     1501 acaacatcaa gcgggtgatg gcggtgctgg gaacgaagcc gctgatggag gcgatgcggg
     1561 cctgacgaga tggtgtcccg cacatcgccg ccaactgcgc ccccgcccag ctgcacgcgt
     1621 ttttacacag cctcgactct tagccgacct tggagtggtc tgctttggga gaccagccct
     1681 gccgaatgtg gcagttggtc accagctcag gacaactcct tcgccaaatt cttccaggca
     1741 acgctcgtga tattaggatc tttcatccag gcgggcgtgt tcgagggcgg ctggtcacct
     1801 ttatacatgg cgattacacg ctttcccagt ttcaagctgg tctccacctc ccactttacc
     1861 caatcgcttt ttgcagtgtt ctctgacacg tagacaacgg tcgtcgagga ctggttgatg
     1921 cgctccttta gtcgcgaacg gatatattct gctctctcgc tgtcgtaggg ctctcgcacc
     1981 gagcggtcgt tgaactcgat atcgctgttc tcattcttgg catgagcgcg cacgaggttc
     2041 acctcgtcga tgtccttcat cgagaagctt aggaacacat ttttgcggcc gccttgcagc
     2101 gcctctttgg ctttctgttc aagtcccttg acgtctccga gacggctgta tccgccgccg
     2161 ccgccgctgc cacccatcag cccgcctcct cgaacagaaa ctcccgacac gcgatacatc
     2221 tgccgcacgg ctcggcctcg ccaagatgac atgaatagga accagccaat cccttctcct
     2281 tggccagctg caccacgtcg cgcttatgaa acgctgcaag cggcgccagt acgcgcactg
     2341 gcttggcaac cgccagtgca agcgtcgcct ccgcggatcg gaggaagtca cttgtctggt
     2401 cagggaatag gcttgtgtcc tcatggagca agccaatcga gactgcgtcg gctccgacct
     2461 gagccgcata ggcagcgccc gtgaggagaa acagcaggtt ccgcccggga gtataagcgt
     2521 cctcgtaaac tcgctgacct cggtcggtga ggccggacct tatgagctga ccgtagccgg
     2581 acaagctagc actcacgggc ggcggcaacc ccattcgtgc catgcccttg acgcaagccg
     2641 cgtactcctg agcaagagcg cgctggccgt agtcgatgaa tagcggaaac tgcgttagac
     2701 cttcctcttt ggctaggcag gcaactaaca gagaatccag gccaccggag acgagagtta
     2761 cgacactcac tgcagcctct ccttgctcac tttagaactc gcgacgcgca tcacgatcca
     2821 gacggcccaa tcgaggaacc cgaccggaag gccgaagccg gccgcatggt ctcgcaggat
     2881 aatctcgtca cgacggtagt cgaccatccg acgcgtcggg gcggcatctg ccgttaggcc
     2941 gactattgtc atgtagttga tcacgtgcct gtccaggacg gccaaatcgt agctggcgcc
     3001 tacattccga aggaacatgc tggcctgctt tggacccagc cccggcgcgt gcgtgacgaa
     3061 ccaatctcgc gcgctctcta tctcgtcaaa tccagcgagc aggcctgcga gcccgtcggc
     3121 atggcaacga accttcattg ccgtcgcggc cagttggcgg gcgcgactgt cggggaagcg
     3181 atatcggcgc agcgtgcctt ggatctcgaa gggccttttg agcaattcgg cgagcgcgcc
     3241 ctcgaggcct tcagcgcttg tatgcccttc caaaagcagt ccgcttgctt ccacgcggac
     3301 agcagccaat tgggcgatag aatagggaac ctggctgctg agcacacaac aggctagctc
     3361 ggtccatagg cggtcttcgc tccagggctc gggcgtctcg ccgctgagct gacggtcgat
     3421 ctcggcaccg accacgctaa cggcgtggca gattcgttcc gggctatgac gcgcgatcac
     3481 agtacactcg gcaatgcggc gcgggcctga gcaattgcgc ctttgggaag gagccccgcc
     3541 agaaaagcca caagcgcctt ctcctcctgc gcggcagatc gcagctcctt gagccaagtg
     3601 ttatagaaat cgaggttatg catcacggcc ttcgtcgtat agctgacggc tggatcaagc
     3661 gccgccgatg cggccaccgg agactcggct actgcaagtt ggtagctaaa caattcgaac
     3721 agctgaaaat ggtgcagctg tgcgtgttca gcgtcaaagg cgtatcgaca ccattccaac
     3781 ccatcgaagc agtctgcgcc ggcggcagaa agcagcgcca agctgatcgg gttgcccgcc
     3841 ccaagaacgt gcaccggctg atagcgacct agtcccttca gcgccttgcg aatgatcgac
     3901 atcgttcggg ctcgctcgaa tacgccagag cccaattcgc gctccgggat ggcgatcatc
     3961 gccggagcca aggcctgcgc gacgtgacgc attaccggcc ccgccatctc tgtccgatag
     4021 ctgtcgtcgg gtcggcgcgg cagatgcacg atgggaatga ccggcgccgt cgttaggcgg
     4081 gctgttcgtt tcgccgcagc gatcgcagct ttggcaacct tatcgatgtc gccggtcggg
     4141 ttgagtacgt cgaaggcgaa ggcgagatcg tgcggggtgg ctcgcaacgc cgtctcaaat
     4201 gtctcgaatt tccagtcccc gtcgtcccga cgcgccctct cgtagtttcc cgaatccagc
     4261 agaatcgttg cgcctctctt ccgtcgtctc tccaaggttt ggatcaggct ctttggcgcg
     4321 ttgggcccgg tatcatatgc ggagacaagg atggtctccg cgccaaacat gtcgagggtt
     4381 ctcaacgcgg cggcgggtgc gagacgggtt tcatgggacg agactgagta gaagaaggcg
     4441 gggagatcca cgaatccgtt catcgccagc ctttggggtc gctcgtccgg gcgccgctct
     4501 tcgatggcgg ccctgacgcg ccccatcaag ctgttgagct gcggcaaagt ttctttgccg
     4561 tcccagccaa gcatctcggt cgcctgcagg ctgccgtaac tcatcggcgc atccgctgca
     4621 tgaatgcgga cgggcaaaat ggcgacgccc gctttgcggg cgatctccaa ttcgtcgtgc
     4681 atatgagcat tgaacgtggc cgccgttgac cagataggga taagcacctc gcaggcagtg
     4741 acctccgctt cgatcccagc ccgatagtcg cccgaaacca gatgccggtc ccaccaaacc
     4801 gaccaacgcg tcttcaggac cgcttccagg gcgggcggga tcgcggacgc gtccgatctc
     4861 gcatagatga tgcagatatc cgccaagccg aatccgcccc gtctcacggc tgagacgcca
     4921 gcctcgcgca tggcgaactc gcgttcaagt agcggcgtgt tattagttta gccggcccag
     4981 caagcccagt agagggctgt tgtgaagtcc gtagtgaacc gcgcgtgcgc aggcggactg
     5041 acgccggttt cgcttagaag gattagccgg ttggggtcgg accgcgccgc aatctcagcg
     5101 agatcgtcag ccccaatctg atccgctagg acgagacagg caccgtcgtt gaattggctc
     5161 tctgggggac cgtcccaacg tcgcacgtcg gctccaagtt ggccaagacg ccacgacgcc
     5221 tcttcgaaca gccagcggtt ggctaactgt gtcttcgagc cgatcaggtg cacctttgca
     5281 ctcgggcttg cggtgactgc tcgaccaggg tgcatcgtcc ccgccggcgg caagggtagg
     5341 gcacgcgtgg ccgcatagtg agcgacgctt cgcgaagcaa cggtagcggc ggcggcggca
     5401 tcctgtcgga gatgcgccca agcgtgagtg aacgccgcgc tgaatacgtc gccggtgcca
     5461 agcttgaata ccgcgtcaga ccagaaggca gggatctgcg ttccgacccc atcgcccgca
     5521 tagacgacgg caccgtgaac accgcacttg acgagcacta ccgccgcccc agcgtctagc
     5581 agcgctcgtg cgccgacgct tagatcatct tgccgggtag cggcccgaag ttcgtctgcg
     5641 ttgaggacta gcgccagtgt ctctgccgat gatccatttt ggctatacgg cgcgaagtcc
     5701 cccacgctct gcggatcata gaccacccgc cgaccggaaa cgaccgcatc gccttcgacg
     5761 aacccaaagc gaagcactac ttccccttca acccggagcg ctggctgacg agtgatgctc
     5821 gcgcgttcgg gagccatcgc agggtccgaa agtggatgaa aataggcgaa cgctattgcg
     5881 ctcgcggcat cggtcgcttt cacctcgatt cccgcgaccg ccaacggata taggtcgttc
     5941 tcgcgcgcct tggggaagta ggtgtgtaga atgacgggag cttggccgac tagggcgtgc
     6001 gctgcgcgtc cgccggagcc gtagacagcg cgccaatgcg gtgcctcgca tgtttcgacg
     6061 taacatcctc cggcgacgat gatcgcgttc acgtcacggt ccgcaaatta gccgccacga
     6121 tccccgccca gggcagatcg cctcgcgagc cactgtgtgt gctctcgcga aaacgcctcc
     6181 tcggcgtcgt cgacaaacag cgacagggct tcatcatcgg cgatctccgg ggcgcgggac
     6241 ttcaagatgc caatctcatt cgcagtcaaa gcgtaggatg cacccagctc attgaagcgc
     6301 ttaatctgca tccagccgag cacggaggcg cctagcaaga tgacggggtc cgggtgcgca
     6361 tagatccagt cgggatgctc catacggatc ataaagatcg cgaaggccgc cacgtagagc
     6421 ccaccgagca cgatagcaaa gttgcggccc gtatccgcgt ttatctttga ctttcggccg
     6481 taccaaccca gttgattgtc gatgcgatca cggatgtaga ttgtcagccg ctccgcgtga
     6541 ggcagagcac ggatcgcctc catatctttc gtgatttggt cgccagcgct cgtcttgccg
     6601 tcaaactgcg agccgaggct acgattggcg tccagcagct cctgcagcat tcgccgcaag
     6661 cgctcgcggt gctctccatc ctgcttgaac ggccgggcac ccatagcgaa tttccaggca
     6721 gtggtcttaa ctgactccgc tagggcgcga gcctggtacc agcgctgctc aggtttcagg
     6781 acagttcgca ggactagaag ggtcagcgct ccggcgagaa gcacgccata ggcgaagacg
     6841 tagcgactat ccgcgaccat gttcacgctc atgcacgaca ccatgagcag ggcggtgaac
     6901 tcgccgaaca ccaagccgaa gtagagcgac tgctgacgtc cagcagcacg gtctgcaacg
     6961 gcgtagagtg caggaaattc catggctacc cccaagatgc ggacatacac gaagttcgcc
     7021 cccatcgcga aggtcttccg ctggcggggc taacgtctgc ttgcggcgct ggcctgaacg
     7081 tccgctcctg gcccaacccc gaccctgcca tcgcctccct cgccgcccca aagcgcccac
     7141 ggaaaagggc ggagcctcgc ggctccgccc ttccgaacga ctggggatcg ttctgctgga
     7201 acgcttacgc ggccttcttg cccttgatct gggcgggcgc ggcggtggtg gtcgtgccga
     7261 tggcgatcgt gcggggcttg agggcctccg gcagctcgcg cctgagggcg atcgacagca
     7321 ggccgtcggc caggttggcg tcggtcacca gcacgtagtc ggccagctgg aagcggcgct
     7381 cgaagttccg ctcggccagg ccgcggtgca ggaacttgcg gctgtcgtcg ttggcggcct
     7441 tgcggccggt cacggtcagg aggttctcct tgacctcgat ggtcagctcg tcgggccgga
     7501 agccggcgac ggcgatctcg atgcggtagg cgttctcgtc ggtccgctcg atgttgtagg
     7561 gcggatagcc ggcggcgctg tcctgggtgg cggcggcgtc aagctgcgcg gccagacggt
     7621 cgaagccgac gacggagcga tagaggggcg agaaatcgat cgtacgcatg ggtgcacatc
     7681 cttctttcaa agcaaggttg cggtttgtca tgcaggccgg tggcgagccc gaaagcctcg
     7741 tcgtgacctg ggaggcccgg acatccagcg cctccgtcac atgaggtggg tgagccgttc
     7801 cggcgtttca acccccgcct tgatcgaaag tcgaacgtcg ttacggtccc cgtctggcag
     7861 ggagacggat gatgctcgga cggcgtgagg tgatcacatt cggggcggcg gcgctggcgc
     7921 tggccgcgac cggggcgagg gcgcggaccg gcgaggatgc ggcgct
//
LOCUS       DAORNX010000086         7884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6787_length_7884_cov_6.133350, whole genome shotgun sequence.
ACCESSION   DAORNX010000086 DAORNX010000000
VERSION     DAORNX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_7884_cov_6.133350"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLO65_05455"
     CDS             complement(<1..194)
                     /locus_tag="PLO65_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD27728.1"
                     /translation="MNQLVRSLVIEDLTIVPLTPTIGAEVEGIDLARPLDDATVKALR
                     QALLDWKVLFFRDQDITTEQ"
     gene            278..886
                     /locus_tag="PLO65_05460"
     CDS             278..886
                     /locus_tag="PLO65_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27729.1"
                     /translation="MTAAAAPVRKKPRVDHAARRDEYVRVAARVLLEKGLHTATMRDI
                     ADAAGAAKVLFYRLFPSREALIEAVFEKVAGAIEDAYAAPWEGYGSMVARVLETARRE
                     PAAFLLVFRNCRASQPDWEARLRPLLARTSLPLFKPGPNAPAGAEDRAQKAAGALFGL
                     FLDSMVRWLEDSDGLTDEERIRWWGRIVRENRAAGREAYRLD"
     gene            946..2568
                     /locus_tag="PLO65_05465"
     CDS             946..2568
                     /locus_tag="PLO65_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRD27730.1"
                     /translation="MIPGLMQDVPLLTTAILTHAETAHGRVEIVSRTVEGRVHRYDYA
                     GLAGRARRLARALQDLGVKSGDRVASLAWNTHRHLELFYAAPGIGAVLNTVNPRLFDD
                     QIVYVLEHAESTVLFYDSTFAPLVERLADRFTTVKTFVLMADADHHDAGAVGALNYET
                     LVADQTDAAFPWPRLDENAGAFLCYTSGTTGDPKGVLYSHRAVVLHGLAAGLASAFGL
                     TAFDVVMPCSSLYHATAWGLPFICPINGCKLVLPAEKMDPASLQALIVGEGVTFSGGV
                     PTIWTMYLDWLAQTGQDVGELKRVIIGGSAVPRAMAETFKVKHGVQVLQIWGMTETCP
                     LGVVATPTPSIAALGEDQVDEIIWTRQGRLQFGIELKIMLEDGTEAPRDGETSGALMV
                     RGPWTIQRYFRKDADVTDAEGWFDTGDIATLDQHGFMRITDRAKDVIKTGGEWISSID
                     LENIAVACPGVKIAAVVGIPHPKWEERPLLVIEAHEGRTVTRDEVLDYLRPRIVKWWM
                     PDDVVFAAVPLTATGKIDKKTLRAAWNNHLLD"
     gene            2574..2867
                     /locus_tag="PLO65_05470"
     CDS             2574..2867
                     /locus_tag="PLO65_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27731.1"
                     /translation="MRSLRLMADYQCFPLWEASPGLVGNVDPATLPLSADLRADLLAW
                     AAAFDATLDLDDPARSGFTEAASRARFRLEGESLGQRLREELGPGWPVILDLD"
     gene            complement(2873..3547)
                     /locus_tag="PLO65_05475"
     CDS             complement(2873..3547)
                     /locus_tag="PLO65_05475"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRD27732.1"
                     /translation="MVLTPFAEIRARAAARKGGEAALAALLPTIKSADDLAAIPDDRW
                     LSMLSRCVFQAGFSWTVVEKKWPGTEDAFWGFSPARCRAMSDEEFDALLRDGRIIRNG
                     AKVRAVQNNAAFLMELAAGRGSAATVFARWPADDYVGLLDLLKTRGDRLGGGAGMMAM
                     RFMGRDGWVTSPDMVKALTREGVIDGAPDTKKARKAIQAAFSQWSAEAGTPFAHISRI
                     LAMSVD"
     gene            complement(3625..4143)
                     /locus_tag="PLO65_05480"
     CDS             complement(3625..4143)
                     /locus_tag="PLO65_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27733.1"
                     /translation="MALFAAGLPGGGATAGTYRCLGGAHVLSDGRCSDGSMAIYEATP
                     MVPDGVGGRQQPGPARPAPADLTGAWSTAVPGAVWQSPSAIPGWNTLHVSPGALAGLL
                     VIYPDGRYVWNAYGGKRGLWRPSGDPEYPVLLDDRAERRLWKVGPNLRRPGRIFIVES
                     NGYFYYEGWRAR"
     gene            4322..4657
                     /locus_tag="PLO65_05485"
     CDS             4322..4657
                     /locus_tag="PLO65_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27734.1"
                     /translation="MKIILSAALCVGLLVASPAAARSDSITFDLKNATRHHLTNLYIS
                     APSTNDWEEDILGDEVANPGETIEVTIDDDLEGCRYDLRADFSDGDSVQIAGVDLCEA
                     DGDTITISE"
     gene            complement(4675..4765)
                     /locus_tag="PLO65_05490"
     tRNA            complement(4675..4765)
                     /locus_tag="PLO65_05490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4729..4731),aa:Ser,seq:gct)
     gene            4888..6462
                     /locus_tag="PLO65_05495"
     CDS             4888..6462
                     /locus_tag="PLO65_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRD27735.1"
                     /translation="MTAPVPREEPQARPEEGWVDAVRALHRVLETQFGPDADTPPIED
                     PRDRRGPFRPDRLVPVRSRLELRHVTHTFRLIDIGAAVLSGLLAAHFMAVDGLLQLTL
                     AQALPLVVSVGLLVWGLRALDAYGFARSEGLSRHLLRVCAAFSLAGLPLALLDLVTGR
                     LPAVLPAWFLGGLISLALLHGLWWSIVMDWRRDGKLTPNIVIVGATAAARKLIETALA
                     TREVAVLGVFDDRLGRAPKDIAGVPVLGDTSDLLGHKIMPYVDRIVIAVNPSAQPRIR
                     QLIDRLRVLPNDVSLVLDAGGEDGRQRALSRIADAQLAQVSGEPADIRRAGAKRAQDL
                     VVGAAALLLGWPLMVAVAIAIKLDSPGPVFFRQRRHGFNNEEILVWKFRSMRAEAADF
                     AAVQQVRAGDDRVTRVGRFIRKTSLDELPQLFNVLAGEMSLVGPRPHAVGMKTGQTES
                     ARLVAEYAHRHRMKPGMTGWAAIHGSRGPVDTPELVRRRVALDIEYIERQSFWLDLYI
                     MAMTIPCLLGDQEAVR"
     gene            6467..>7556
                     /locus_tag="PLO65_05500"
     CDS             6467..>7556
                     /locus_tag="PLO65_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRD27736.1"
                     /translation="MFRRGLIGYLPANIVQGLVGLLTIVVFTRLLTPEQYGGYALALS
                     VMTLAHTTLFTWVEAAMARFQARAVARGDAADHLATLLRAFAVLSLVFPVLATLAVWL
                     TPMSPALRLAVAVGLGAIIFRSLIRLVQEHRRADGDVAAASALDMTQTAGGFAIGVLA
                     ITLGAGAAGPLIGMAAIAVIGLVFSLPKELALARGGRFERSRLADYASYGLPVSLSLI
                     LALVIATTDRFLLAAFLDERAVGVYHAGYSLANRTLDVMFIWLGMAGGPAMVAALERG
                     GGPALDATAREQAAFMILLTLPAAVGLALVAHPLAEVMVGGPLVEGAAKVTPWVAFGA
                     FFSGVTTYYLHQAFTLGRRTHLLFAAMII"
     assembly_gap    7557..7609
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7610..>7884
                     /locus_tag="PLO65_05505"
     CDS             <7610..>7884
                     /locus_tag="PLO65_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRD27737.1"
                     /translation="TGAMWSTAASYAVGAVMSWLLGRRAMPLPVPLDVIWRAGLACAV
                     MVLAVMRIPALGGLLELGLKAAVGGLVYAAVVLLLDAGGLRTRGAGLL"
BASE COUNT         1118 a         2821 c         2684 g         1208 t
ORIGIN      
        1 tgctgctcgg tggtgatgtc ctggtcgcgg aagaacagca ccttccagtc cagcagggcc
       61 tggcgcaggg ccttcacggt cgcgtcgtcc agcggccggg ccagatcgat gccctcgacc
      121 tccgcgccga tggtcggcgt cagcggaacg atggtcagat cttcgatcac gaggctgcgg
      181 acaagctggt tcacgccggt atcctcccgt ttcggccaga gtatgttacc aaggagtttc
      241 attcggcaag cgctgatcgt tccgctagac ctgagccatg accgcagccg ccgcgcccgt
      301 ccgcaagaag ccccgcgtcg accacgccgc gcgtcgcgac gaatacgtcc gcgtggcggc
      361 gcgcgtgctg ctcgaaaagg gcctgcacac ggcgaccatg cgcgacatcg ccgatgcggc
      421 cggcgcggcc aaggtgctgt tctaccggct gtttccgtcg cgcgaggcgt tgatcgaggc
      481 cgtattcgag aaggtcgccg gcgcgatcga ggacgcctac gccgcgccgt gggagggcta
      541 tgggtcgatg gtcgcccggg tgctcgagac cgcgcgccgc gagccggcgg ccttcctgct
      601 ggtgttccgc aactgccgcg ccagccagcc ggactgggag gcgcggctgc ggccgctgct
      661 ggcgcggacg tccctgcccc tgttcaagcc gggccccaac gccccggcgg gcgccgagga
      721 ccgggcccag aaggccgccg gggccctgtt cggcctgttc ctcgacagca tggtccggtg
      781 gctggaggac agcgacggcc tgaccgacga ggagcgcatc cgctggtggg gccgcatcgt
      841 gcgcgagaac cgcgccgccg ggcgcgaggc ctaccgtctg gattgacttg cacggcggcc
      901 agcgccgatc ctgaacgcca atcacaagac caagaggaaa cgcccgtgat ccccggattg
      961 atgcaggacg tcccgttgct gacgaccgcc atcctgaccc acgccgagac ggcgcacggc
     1021 cgggtggaga tcgtctcgcg caccgtggag ggccgcgtcc atcgctacga ctacgccggc
     1081 ctcgccggcc gcgcgcgacg gctcgcccgg gcgctgcagg acctgggcgt caagtccggc
     1141 gaccgggtcg cgagcctggc ctggaacacc caccgacacc tggaactatt ctacgccgcg
     1201 ccggggatcg gcgcggtgct caacacggtc aatccgcggc tgttcgacga ccagatcgtc
     1261 tatgtgctcg aacacgccga aagcacggtg ctgttctacg acagcacgtt cgcgcccctg
     1321 gtcgagcggc tggccgaccg gttcaccacc gtgaagacct tcgtgctgat ggccgacgcc
     1381 gatcaccacg acgccggcgc ggtcggcgcg ctgaactacg agaccctggt cgcggaccag
     1441 accgacgcgg cctttccctg gccgcggctc gacgagaacg ccggcgcctt cctctgctac
     1501 acctcgggga ccacggggga ccccaagggc gtgctctaca gccatcgcgc cgtggtgctc
     1561 cacgggctgg cggcgggcct ggccagcgcc ttcggcctga ccgccttcga cgtggtcatg
     1621 ccctgctcgt cgctctacca cgccacggcc tggggcctgc cgttcatctg cccgatcaac
     1681 ggctgcaagc tggtgctgcc ggcggaaaag atggacccgg ccagcctgca ggccctgatc
     1741 gtcggcgagg gcgtgacctt ttccggcggc gtgccgacca tctggaccat gtacctcgac
     1801 tggctggccc agaccggcca ggacgtcggc gagctcaagc gggtgatcat cggcggctcg
     1861 gccgtgccgc gcgccatggc cgagaccttc aaggtcaagc acggcgtgca ggtgctgcag
     1921 atctggggca tgaccgagac ctgcccgctg ggcgtggtcg ccacaccgac gccctcgatc
     1981 gccgcgctgg gcgaggacca ggtcgacgag atcatctgga cccgccaggg tcggctgcag
     2041 ttcggcatcg agttgaagat catgctcgag gacggaacgg aggcgccgcg cgacggcgag
     2101 accagcggcg ccctgatggt gcgcggcccc tggaccatcc agcgctactt ccgcaaggac
     2161 gccgacgtga cggacgccga gggctggttc gacaccggcg acatcgccac cctggaccag
     2221 cacggcttca tgcgcatcac cgaccgcgcc aaggacgtga tcaagaccgg cggcgaatgg
     2281 atcagctcga tcgacctgga gaacatcgcc gtcgcctgcc ccggggtgaa gatcgccgcc
     2341 gtggtcggca ttccccatcc caagtgggag gaacggccgc tgctggtgat cgaggcccac
     2401 gagggccgca cggtgacccg cgacgaggtg ctcgactacc tgcggccgcg gatcgtgaag
     2461 tggtggatgc cggatgacgt ggtcttcgcc gccgtgcccc tgaccgccac cggcaagatc
     2521 gacaagaaga ccctgcgggc ggcgtggaac aatcacctgc tggactgagc cgcatgcgat
     2581 cgctgcggct catggccgac taccagtgct ttcccctctg ggaggcgtcg cccgggctgg
     2641 tgggcaacgt cgacccggcg acccttccgc tgtcggcgga cctgcgcgcc gatctcctgg
     2701 cctgggccgc ggcgttcgac gccaccctcg acctcgacga ccccgcaagg tcgggcttca
     2761 ccgaggcggc gtcgcgggcg cgtttccggc tggaaggcga aagcctcggc cagcggttgc
     2821 gcgaggagct tggccccggc tggccggtga tcctcgatct ggactgaacg cctcagtcca
     2881 cgctcatcgc caggatccgg ctgatgtggg cgaacggcgt ccccgcctcg gccgaccact
     2941 ggctgaaggc cgcctggatc gccttgcggg ccttcttcgt gtccggcgcg ccgtcgatga
     3001 cgccctcgcg ggtcagcgcc ttgaccatgt ccggcgaggt cacccagccg tcgcggccca
     3061 tgaagcgcat ggccatcatg cccgcgccgc cgcccagccg gtcgccgcgc gtcttcagca
     3121 ggtcgagcag gccgacatag tcgtccgccg gccagcgcgc gaacaccgtc gccgccgagc
     3181 cccgcccggc cgccagctcc atcaggaatg cggcgttatt ctgcaccgcc cgcaccttgg
     3241 cgccgttgcg gatgatccgg ccgtccctga gcagggcgtc gaattcctcg tccgacatcg
     3301 cccggcaacg ggccggcgag aagccccaga aggcgtcctc cgtccccggc catttcttct
     3361 cgaccaccgt ccaggagaag ccggcctgga acacgcaacg gctgagcatc gacagccagc
     3421 ggtcgtcggg aatggccgcc aggtcatcgg cggacttgat cgtcggcagc agcgccgcca
     3481 gcgccgcctc gccgcccttg cgcgcggccg ccctggcgcg aatctccgcg aatggcgtca
     3541 agaccatgga catcctccgg cgcggcctgc ccgggaccgc cggagccatc ctgaggccgg
     3601 gcggtggctc gcgccagtcc tgcgctaacg ggcccgccag ccctcatagt agaagtagcc
     3661 gttgctctcg acgatgaata tccgtccggg ccggcgcagg ttgggaccga ccttccacag
     3721 gcgccgctcg gcccggtcgt cgagcagcac cggatattcc ggatcgcccg atggccgcca
     3781 gagaccccgc ttgccgccat aggcgttcca gacgtagcgg ccgtccggat agatgaccag
     3841 cagaccggcc agggcgcccg gcgagacatg cagggtgttc cagccgggaa tcgccgatgg
     3901 cgactgccag acggcgccag gcacggcggt cgaccaggcg ccggtcagat cggcgggcgc
     3961 cggcctcgcg ggcccgggct gctggcgacc cccgacgccg tcgggaacca tcggcgtggc
     4021 ctcgtagatc gccatcgagc cgtcggaaca tcggccgtca gacagcacgt gcgcgccgcc
     4081 cagacagcgg taggtcccgg cggtcgcgcc gccgcccggc agccccgcgg cgaaaagcgc
     4141 catgacgaga atctcgcgcc tgcccatctc cgacctcccc tgcctgttca gggtcttcag
     4201 aagaggtccc ctcgcccggc ggggcgttga cgcaggtcaa caggccgaaa tgatcgacag
     4261 agtctggacc atgtcctagc tatggcccgg tcagcgcttg gctgacgggg gctttccttc
     4321 catgaaaatc attctgtccg cggcgctttg cgtcggtctg ctcgtcgcca gcccggccgc
     4381 ggcccgatca gactcgatca ctttcgacct gaagaacgcg acgcgccacc acctgaccaa
     4441 cctctacatt tccgcgccgt cgaccaacga ctgggaagag gacatcctcg gcgacgaggt
     4501 cgccaacccc ggcgaaacca tcgaggtgac catcgacgac gatctggagg gctgcagata
     4561 cgacctgagg gccgacttct ccgacggcga ttcggtgcag atcgccgggg tcgacctgtg
     4621 cgaggccgac ggcgacacca tcacgatcag cgaataggga tcaccccggc gaggtggcgg
     4681 agacggtggg attcgaaccc acggtaccct tttggggtac gctcatttag caaacgagtc
     4741 ccttcggcca ctcgggcacg tctccgtccg ctcgcgcggg acgcctctca tatccaagtg
     4801 ccgcggcgtt cgcaacgacg gcgcgcacgg attgggtgaa ccctgttaac cggacgctca
     4861 gccggctgtg ccagactggt cgggatgatg accgctcctg tcccgcgcga agagccgcag
     4921 gcccgtcccg aagaaggctg ggttgacgcc gtgcgcgccc tgcaccgggt gctcgagacc
     4981 cagttcgggc cggacgccga caccccgccg atcgaggatc cgcgcgaccg gcgcggtccg
     5041 ttccggccgg accggctggt gccggtgcga tcgcggctcg aactgcggca cgtgacccat
     5101 accttccggc tgatcgacat cggcgcggcg gtgctgtccg gcctgctggc cgcccacttc
     5161 atggccgtcg acggcctgct gcagctgacc ctggcccagg cgctgccgct ggtcgtcagc
     5221 gtgggcctct tggtgtgggg actgcgggcg ctggacgcct acgggttcgc gcgcagcgag
     5281 ggtctgtcgc ggcacctgct gcgcgtctgc gccgccttca gcctcgccgg cctgccgctg
     5341 gccctgctgg acctggtcac gggacgcctg cccgcggtcc tgccggcatg gtttctcggc
     5401 ggcctgatca gcctggcgtt gttgcacggc ctgtggtggt cgatcgtcat ggactggcgg
     5461 cgcgacggca aactgacccc gaacatcgtc atcgtcgggg ccacggcggc ggccaggaag
     5521 ctgatcgaga cggcgctggc gacccgcgaa gtcgcggtgc tgggtgtctt cgacgaccgg
     5581 ctcggccggg cgccgaagga catcgccggc gtgccggtgc tgggcgacac ctccgacctg
     5641 ctcggtcaca agatcatgcc ctatgtcgac cgcatcgtga tcgcggtgaa tccctccgcc
     5701 cagccgcgaa ttcgccagct gatcgaccgg ctgagggtgc tgcccaacga cgtcagcctg
     5761 gtgctcgacg ccggtggcga ggatggacgc cagcgggccc tgtcgcggat cgccgacgcc
     5821 cagctggccc aggtctcggg cgagcccgcc gacatccgcc gggccggcgc caagcgcgcc
     5881 caggacctgg tggtcggggc cgcggccctg ctgctgggct ggccgctgat ggtcgcggtg
     5941 gccatcgcca tcaagctcga cagtccaggg ccggtgttct tccgccagcg ccgccacggc
     6001 ttcaacaacg aggagatcct cgtctggaaa ttccgctcga tgcgggccga ggcggccgat
     6061 ttcgccgccg tgcagcaggt tcgcgcgggc gacgaccgcg tcacccgggt gggccggttc
     6121 atccgcaaga ccagcctcga cgaactgccg cagctgttca acgtgctggc cggcgagatg
     6181 agcctggtcg gtccccgccc ccacgccgtc ggcatgaaga ccggccagac cgagtccgcc
     6241 cgcctggtgg ccgagtacgc ccaccgtcac cggatgaagc cgggcatgac cggctgggcc
     6301 gccatccatg gctcgcgcgg gcccgtcgac acgcccgagc tggtccgccg ccgcgtggcg
     6361 ctcgacatcg agtacatcga acgccagtcg ttctggctcg acctctacat catggccatg
     6421 accatcccct gcctgctcgg ggaccaggaa gcagtgcggt aggccgatgt ttcgacgcgg
     6481 cctgatcggc tacctgcccg ccaacatcgt ccagggcctg gtcggcctgc tgaccatcgt
     6541 ggtgttcacc cgcctgctga cgcccgagca gtacggaggc tacgcgctgg cgctgtcggt
     6601 gatgacactg gcccacacca ccctgttcac ctgggtcgag gcggccatgg cccggttcca
     6661 ggcccgcgcg gtggcccgcg gcgacgcggc cgaccatctg gcgacgctgc ttcgcgcctt
     6721 cgccgtgctg tccctggtgt ttccggtcct cgccaccctg gccgtctggc tgacgccgat
     6781 gagcccggcg ctgaggctgg ccgtggccgt gggcctcggc gcgatcatct tccgcagcct
     6841 gatccgcctt gttcaggagc accgccgcgc cgatggcgac gtggccgccg cctcggcgct
     6901 cgacatgacc cagaccgccg gcggcttcgc catcggggtg ctggccatca ccctcggcgc
     6961 cggcgcggcc ggtccgctga tcggcatggc cgcgatcgcc gtgatcggac tggtcttcag
     7021 cctgcccaag gaactggccc tggcgcgcgg cggccggttc gagcggtccc ggctggcgga
     7081 ctacgccagc tacggcctgc ccgtgtcgct gtcgctgatc ctggccctgg tcatcgccac
     7141 caccgaccgg ttcctgctgg cggcctttct cgatgagcgg gcggtcggcg tctatcacgc
     7201 gggctatagc ctggcgaacc ggacgctcga cgtgatgttc atctggctgg gcatggccgg
     7261 gggaccggcg atggtcgcgg cgctcgagcg cggcggggga ccggcgctcg acgccacggc
     7321 ccgcgagcag gccgccttca tgatcctgtt gaccctgccg gcggctgtcg gcctggccct
     7381 ggtcgcccac cccctggccg aggtgatggt tggcgggccg ctggtcgagg gcgccgcgaa
     7441 ggtgaccccg tgggtggcct tcggcgcctt cttctccggc gtgacgacct actacctgca
     7501 ccaggccttc acgctgggac gccggaccca tctgctgttc gcggcgatga tcattcnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccggggccat
     7621 gtggtcgacc gcggccagct acgcggtggg cgcggtcatg tcctggctgc tcggccgccg
     7681 ggccatgccc ctgcccgtgc cgctggacgt gatctggcgc gccggcctgg cctgcgccgt
     7741 catggtcctg gccgtgatgc ggattccggc gctgggcgga ctgctcgaac tgggactcaa
     7801 ggccgctgtc ggcgggctgg tctacgccgc cgtcgtcctg ctgctggacg cgggcggcct
     7861 gcgcacgcgc ggcgcaggcc tgct
//
LOCUS       DAORNX010000087         7878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6792_length_7878_cov_6.646683, whole genome shotgun sequence.
ACCESSION   DAORNX010000087 DAORNX010000000
VERSION     DAORNX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6792_length_7878_cov_6.646683"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1163)
                     /locus_tag="PLO65_05510"
     CDS             complement(63..1163)
                     /locus_tag="PLO65_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD27738.1"
                     /translation="MRRSEPVSWIPALDIYYVTRHADVSAILMDDATFLVGEENMLVY
                     DTFGRHMMTVDGAEQRRFRAGFRRTFTPRVIRERLEEKVTALVHGLIDGFAGGGAVEL
                     RAAFASRLPVQVMLAVFGLPAEDEPLLRRWYDAFERSLSNYRWDPAIRAEGRRCVEEF
                     KAHLQRRLAAASALPADSLLAALTQDDSEGRLSDDEILQNALIIFFGGISTVEALVLN
                     VFHALSRHESVFGRLRADPSVLPRVIEETARWSSPVQAVTRYVGQDVELHGVRIARGR
                     LVNCMLGSANRDEAVFANPDSFDIDRDDLGRHIAFATGPHVCLGSHLARAEARIAITA
                     LLARLPGLRVADVAELEGYEFRQPGHLTLAWD"
     gene            complement(1243..2178)
                     /locus_tag="PLO65_05515"
     CDS             complement(1243..2178)
                     /locus_tag="PLO65_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRD27739.1"
                     /translation="MRRADKVIITAAVTGSIHTPSMSPYLPITPDEIAADAIGAAEAG
                     AAILHLHARDPRDGRPTADPDVFMQFLPRIKQGTDAAINLTTGGAPGMTMEERLAAPH
                     RARPEITSLNMGTLNFGVFDSATKIKDWKYDWEKPFLEGSREGFQSNTFAQIERVIRD
                     LHHGYGTRFEYECYDVGHLYNLAHFVDRGLLKPPFFIQMIFGILGGIGADLDHLTHMR
                     NTADRLFGDDYYLSCFAVGRDQMKFLTTSAILGGSVRCGLEDSLVLSRGTLAKSSGEQ
                     VLKIRRIIEELGLEVATPAEARAMLQLKGADLVGF"
     gene            2294..3019
                     /locus_tag="PLO65_05520"
     CDS             2294..3019
                     /locus_tag="PLO65_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD27740.1"
                     /translation="MDGKARLARISRETQQERVFRELSRGLLAGVFAPGEVISIRALC
                     AQLGAGAMPVRESVQRLVGQGALEALPNRTLRIPEMSAEAFEDLMRLRAALEPMAAGL
                     AAESADAEDIERFHRLHVDLERAVRARNVSEALRLNTELHFAIYQAAGAPMLAAVIEG
                     LWLRLGPLLRVIFSSPDVLDSIFDQIWSRHSNLDQALANRNPAGAQAALAQIIALTDR
                     WFAHNHPFVDGEGPAAAKPRVDQ"
     gene            3016..3771
                     /locus_tag="PLO65_05525"
     CDS             3016..3771
                     /locus_tag="PLO65_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27741.1"
                     /translation="MKALVIGGGDSVGRASAEAMLARGDRVHILDVRAEAVAATLAAN
                     SSMSGAVADFGAPPAIERAMSEALGLLGGVDALVYTVGLPGPVAAIEDIALADWDRCW
                     AVNVTGLLMAIQLVTPSMKARGGGSIVAFSTSSTRTRLPRRTPYVVTKFALEGLVLNA
                     ARELGPDGIRVNAIQPGMIDNARMRGIIDRRATEEGVAPAEIEARFTRYISLRASVSL
                     TDLAESVLFLTSPAAGRITGELLAVSGNAEWED"
     gene            3880..>4857
                     /locus_tag="PLO65_05530"
     CDS             3880..>4857
                     /locus_tag="PLO65_05530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27742.1"
                     /translation="MKRHLPMVLAVLAIASGAAADPAPTSSCPRLDPVFSRWAGEDSP
                     GFAVAVVSDGRLVCEQAYGKADLASGAAITPDTRFNLASLTKPFVALAAVDVARAGAL
                     DLDAPLARVLPEAAPYYHSATTRQMLSHTAGLPDIATLIVLAGRSSAEPLENGQLRDL
                     IDRQDHLNFAPGARYLYSNSGYLLAAAVLERATGKPYAGIMADRVFRPAGMVAPQIYA
                     APGQAVAGAARSYAFDAGDQTWRPIAYASAAHGSTNLYASADDVARWAAWFLAAARAR
                     DPRVAEMTRPAPVTGGAASYGLGLELIAYRGRTLWMHTGSEAGYRLVLLM"
     assembly_gap    4858..5013
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5014..5539
                     /locus_tag="PLO65_05535"
     CDS             <5014..5539
                     /locus_tag="PLO65_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27743.1"
                     /translation="AAAFSGFYELEPGRVAQVAYADNRVFVAVDPLGVAAFDAVSATR
                     LLHRGSGVAVTFDRPGVGGFDRLRLSGLGPPSAGRRVAVATTDPTTLGRLAGRYGSTT
                     LGATYDVTAGAHGLSLILQGQTEPLPLTALADGKFVSAEAGATVEFLDDRTFLLSTWR
                     VNDIRFERLPQQAN"
     gene            5542..6699
                     /locus_tag="PLO65_05540"
     CDS             5542..6699
                     /locus_tag="PLO65_05540"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRD27744.1"
                     /translation="MQPDAIPLIPLTGDAYERGLTHGRVCADEIHQILADWRRHVAPA
                     AGSADAYREAFRSRTDFLKGFQDHAPHLIDELRGIAEGARVPFGQILDLQLLDEEWWF
                     RQLYSQTSAAHPHHCSALALRTTGGGVLVAQNFDGASWMEGYQRVFHHRDPVTGLDAL
                     IFSLPGILALNGINRHGVAVCVNSLVQLDGDPCGLPVVGVIRRLLEAPDQPAAVATLR
                     SVRHASGQTYTLGDSRTFGAFEVSRSSIVEVGGLPRLPAVCHTNHPLANPDTGLFQRA
                     TAGDTPERRLARTANSAARLAALEQGLGDSAAYSVDGVKALLASRRHPDHPLSRELGG
                     GGEVIDYTLACLVFEPGPPALMHLAAGPPSTTGFRTFICDSGVAPLARTLP"
     gene            6696..7829
                     /locus_tag="PLO65_05545"
     CDS             6696..7829
                     /locus_tag="PLO65_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRD27745.1"
                     /translation="MIASDQSFALLAVIALSAAFGYWIDSTPWGKRIPGILLAIVIPM
                     ALRTGGILPGDAPLYDTLGRFLIPLAVPLVLFQADFRRIYRLAGPMLLIFALGTIATL
                     AAVFLGVALFDLGPDAAAFAAVLAASDIGGSSNLIAVSQIVDWASPASLPVALAADAL
                     AGTTYWMIILALPMAPFLRRFFRSPIMDDVERDKDAPTMLSAPSSSAHMMVNFGASAL
                     IVALSTALAARLGAPHLSILVISGMALVVANLAPQFFRTDRSAEEAGMILIYLFMPVI
                     GALTDLRSLTSTSLNVVGFALTIVLTHLLLMGVIAKLFRFDIAEALIASCAGILGPPS
                     TVATAATFDWRALVVPGFMCAVLGNAIANFIGVAVYRAVQALP"
BASE COUNT         1109 a         2796 c         2563 g         1254 t
ORIGIN      
        1 ctcgacggcc cgatcccggg cttgaacagg tcgaagacgc tgacggtcat cgtgccggcg
       61 actcagtccc aggccagggt caggtggccc ggttggcgga actcgtagcc ctccagctcg
      121 gcgacatcgg cgacgcgcag cccgggcagc cgcgcgagca gggcggtgat ggcgatccgg
      181 gcctcggccc gcgcgagatg ggatccgaga cagacgtggg gacccgtggc gaaggcgatg
      241 tgccggccga gatcatcgcg gtcgatgtcg aagctgtcgg gattggcgaa caccgcttca
      301 tcccggttcg cgctgccgag catgcagttg accagccgcc ccctggcgat gcggaccccg
      361 tgcagctcga catcctgccc cacatagcgg gtcacggcct ggaccggcga ggaccagcgg
      421 gcggtctcct cgatgacccg gggcaggacc gacgggtcgg cgcgcaggcg tccgaacacg
      481 gactcatgcc gggacagggc gtgaaacacg ttcagcacga gcgcctcgac cgtcgaaatt
      541 cccccgaaga agatgatcag ggcgttctgc aggatctcat cgtcgctcag ccggccctcg
      601 gagtcgtcct gggtcagggc ggccagcagg ctgtcggccg gcaaggccga ggccgccgcg
      661 agccgccgct gcaggtgggc cttgaactcc tcgacgcatc ggcggccctc ggcccggatc
      721 gcggggtccc accggtagtt cgacaacgac cgctcgaagg cgtcgtacca ccggcgcaac
      781 agcggttcgt cctcggccgg cagtccgaaa accgcgagca tgacctggac cggcaggcgc
      841 gacgcgaacg ccgcgcgcaa ctccaccgcg ccgccaccgg cgaagccgtc gatgagaccg
      901 tgaaccaggg cggtgacctt ctcctccagc cgctcgcgga tcaccctggg cgtgaacgtc
      961 cggcggaagc cggcgcgaaa gcggcgctgc tcagcaccgt cgactgtcat catgtggcgg
     1021 ccgaaggtgt cgtagacgag catgttctcc tcgccgacga ggaaggtcgc gtcgtccatc
     1081 aggatcgcgc tcacgtcggc gtgccgcgtc acatagtaga tatcgagcgc cgggatccag
     1141 gacaccggct cggaccgtcg catgcgcgcg aacgtcggat gcggatcggc gctgaaggcg
     1201 gccaggtcaa tcgaggcgcc gacgggatca tctccccccg gatcagaacc cgacaaggtc
     1261 ggcccccttg agctgcagca tcgcgcgcgc ctccgccggg gtcgcgacct ccaggcccag
     1321 ctcttcgatg atccggcgga tcttgagcac ctgctcgccg gacgatttcg ccagcgtgcc
     1381 gcggctcagg accaggctgt cctcaaggcc gcagcggacg ctgccgccga ggatggcgct
     1441 ggtggtgagg aacttcatct ggtcgcgccc cacggcgaag caggacaggt agtagtcgtc
     1501 gccgaacagc cggtcggccg tgtttcgcat gtgcgtgaga tgatcgagat ccgcgccgat
     1561 cccgccgagg atgccgaaga tcatctggat gaagaacggc ggcttcagca gcccccgatc
     1621 gacgaaatgg gccaggttgt agaggtggcc gacgtcataa cattcatact cgaagcgcgt
     1681 gccgtagccg tggtgaagat cccggatgac ccgctcgatc tgggcgaagg tgttcgactg
     1741 gaacccctcg cgactgcctt cgaggaaggg tttttcccag tcgtacttcc agtccttgat
     1801 cttggtggcg gagtcaaaga cgccgaagtt gagcgtgccc atgttcagtg aagtgatctc
     1861 gggcctggcg cgatgcggcg ccgccagccg ctcctccatg gtcatgccgg gcgcgccgcc
     1921 ggtggtcagg ttgatggccg cgtcggtccc ctgtttgatg cgcggcagga attgcatgaa
     1981 cacgtcggga tcagctgtcg ggcggccgtc gcgcgggtcg cgggcatgca ggtgcaggat
     2041 ggccgccccg gcctcggccg caccgatggc gtccgccgcg atctcgtccg gcgtgatggg
     2101 caggtagggc gacatgctgg gcgtgtggat cgacccggtc acggccgccg tgatgatgac
     2161 cttgtccgcc ctgcgcatcc ccatctcctt ttggattatc gatttgatca aatcgatacg
     2221 attgacgcta tcccgcgcag ccgatcgagt ctaggcttga tctcgccccc gatttggaag
     2281 gacaggcccg ctcatggacg gaaaggcccg gctcgcgagg atttcccgcg aaacgcagca
     2341 ggagcgcgta ttcagggaac tgtcccgggg gctgctggcc ggggtcttcg cgccgggaga
     2401 agtcatctcc attcgggccc tctgcgccca gctcggcgcc ggggcgatgc cggtgcggga
     2461 gagcgtgcag cgcctggtcg gacagggcgc gctcgaggcg ctcccgaacc ggacgctgcg
     2521 tatcccggag atgtcggcgg aagccttcga ggacctgatg cggttacgcg ccgcgctcga
     2581 gccgatggcc gccggtctgg ccgccgagag cgccgacgcc gaggacatcg agcgcttcca
     2641 ccggctgcac gtcgacctcg aacgggcggt gcgcgcacgc aacgtcagcg aggcgctgcg
     2701 gctcaatacc gaactccact tcgccatcta ccaagcggcc ggcgcgccga tgctggcggc
     2761 ggtcatcgag ggcctctggc tgcgcctggg gccgctgttg cgcgtcatct tctcctctcc
     2821 cgacgtgctc gacagcatct tcgaccagat ctggagccgg cattcgaacc ttgatcaggc
     2881 cctcgccaac cggaacccgg ccggcgccca ggccgccctg gcgcagatca tcgccctgac
     2941 tgaccgctgg ttcgcccaca accacccgtt cgttgacggc gaaggtcccg ccgccgccaa
     3001 gccacgggtc gaccagtgaa ggcgctcgtc atcggcggcg gcgacagcgt cggacgcgcc
     3061 tcggccgagg ccatgctcgc gcggggcgat cgcgtgcata tcctcgatgt ccgcgccgag
     3121 gccgtcgccg cgaccctggc ggccaattcc tcgatgtcgg gcgcggtcgc cgactttggc
     3181 gcccccccgg ccatcgagcg ggcgatgtcg gaggctctgg gcctgctcgg gggcgttgac
     3241 gccctggtct acaccgtcgg actgcccggg ccggtcgcgg cgatcgagga catcgcgctc
     3301 gccgactggg accgctgctg ggccgtcaat gtcacgggcc tgctcatggc aatccagctc
     3361 gtgacacctt ccatgaaggc ccggggcggc gggtcgatcg tggccttctc gacaagctcc
     3421 acccgcaccc gtctgcccag gcggacgccc tatgtggtga ccaagttcgc gcttgagggg
     3481 ctcgtgctca acgcggcccg cgagctcggc ccggacggga tccgggtcaa cgccatccag
     3541 cccggcatga tcgacaacgc caggatgcgc ggcatcattg accgcagggc gaccgaagaa
     3601 ggcgtcgcgc ccgccgaaat cgaggcgcgc ttcacccgct acatctcgct tcgcgcatcg
     3661 gtctccctca ccgatctcgc cgagtcggtg ctgtttctca cctcgccggc ggccgggcgc
     3721 atcaccggcg aactgctggc ggtcagcggc aacgccgagt gggaggacta gggaccaccg
     3781 gttgccccat gatccacggg accccccagg ttcgattgac caagattgat tatgacatca
     3841 tagtcaattc cagacgacgc cgcgaccctg gagatcctcg tgaagcgaca cctgccgatg
     3901 gtcctggcgg tcctggcgat cgcctcaggc gcggcggccg atccggcgcc gacgagctca
     3961 tgcccccggc tggacccggt gttttcccga tgggccggcg aggattcgcc cggcttcgcc
     4021 gtggcggtgg tgtcggacgg ccggctcgtc tgtgaacagg cctatggcaa ggcggacctg
     4081 gcctccggcg ccgccatcac gccggacaca cggttcaatc tcgcttccct gaccaagccg
     4141 ttcgtcgcgc tggccgcggt cgatgtcgcg cgcgccgggg cgctcgatct ggatgctccc
     4201 ctcgcccggg tcctgccaga ggccgcgccc tactaccact cggcgacgac ccggcagatg
     4261 ctgtcgcata cggccggact ccccgacatc gccaccctca tcgtgctggc gggccggtca
     4321 tcggccgaac cgctcgagaa tggccagctg cgcgacctga tcgaccgcca ggaccacctg
     4381 aacttcgcgc ctggcgcccg ctacctgtac tcgaacagcg gctacctgct ggcggccgcc
     4441 gtgctggaac gggccaccgg caagccctac gccggcatca tggccgaccg cgtcttccgg
     4501 ccggccggga tggtcgcgcc gcagatctac gccgcgcccg gccaggccgt cgccggcgcg
     4561 gcccggtcct acgccttcga cgccggcgac cagacctggc ggccgatcgc ctatgcgtcg
     4621 gcggcccacg gcagcaccaa cctctatgcg tcggctgacg acgtcgcccg ctgggcggcc
     4681 tggttcctcg cggccgccag ggctcgcgat ccccgcgtcg cggagatgac ccggcccgcc
     4741 ccggtgacgg gcggagccgc ctcctatggc cttggcctgg agctcatcgc gtaccgcggg
     4801 cggacactct ggatgcatac cggctcggaa gccggctacc ggttggtgct gctgatgnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcggcc gccttcagcg gcttctacga
     5041 gctcgaaccc ggccgcgtcg cccaggtcgc ctatgccgac aaccgggtct tcgtggccgt
     5101 cgacccgctc ggggtcgcag ccttcgacgc cgtctccgcc acgcggctgc tgcaccgcgg
     5161 cagcggggtt gcggtcacgt tcgaccggcc gggcgtgggg ggcttcgacc gtctccggct
     5221 gtcgggcctc ggcccaccgt ccgcaggcag gcgggtggcc gtcgccacaa ccgaccccac
     5281 gaccctcggc cgcctcgccg ggcggtatgg ttcgaccacc ctcggcgcga cctacgacgt
     5341 gaccgccggc gcgcatggcc tgtccctgat cctgcagggc cagaccgagc cccttcccct
     5401 gaccgccctg gccgacggca agttcgtctc cgccgaggcc ggcgccacgg tcgagttcct
     5461 cgacgacagg acctttctcc tcagcacctg gcgggtgaac gacattcgct tcgagcgcct
     5521 gccgcagcag gcgaactagg catgcagccc gacgccattc cgctcattcc gctcaccggc
     5581 gacgcctacg agcgcggcct gacgcacggc cgtgtctgcg ccgacgaaat ccatcagatc
     5641 ctggcggact ggcgacgcca tgtcgccccc gctgcgggtt cggccgacgc ctaccgggag
     5701 gcgttccggt cccggaccga cttcctcaag ggcttccagg atcacgcccc gcatctgatc
     5761 gatgaactgc gcggcatcgc cgagggcgcg cgggttccct tcggacagat cctggacctg
     5821 cagttgctcg acgaggaatg gtggttccgg cagctctatt cgcaaacgtc agcggcgcat
     5881 ccgcaccatt gcagcgccct cgcgctccgc accaccggcg gcggcgtcct ggtggcgcag
     5941 aatttcgatg gcgcgagctg gatggagggc taccagcgcg tctttcatca tcgcgacccg
     6001 gtgaccggac ttgacgcgct aatcttctcg ctgccgggca tcctggcgct caacggcatc
     6061 aatcggcacg gggtggcggt gtgcgtcaat tccctggtcc agctcgatgg cgatccctgc
     6121 ggccttccgg tcgtcggcgt catccgccgc ctgctcgagg cgccggacca gccggcggcg
     6181 gtcgccacgc tgcgatcggt gcggcacgcc tcgggacaga cctacaccct cggagactcc
     6241 cggacgttcg gcgccttcga ggtctcgcga tcctcgatcg ttgaagtcgg cggccttcca
     6301 cggcttccgg cggtctgcca caccaaccat ccgctggcga acccggacac cggcctgttc
     6361 caacgcgcga ccgccggtga cacgccggag cggcggctcg cgaggaccgc gaactccgcc
     6421 gcgcgcctgg ccgccctcga gcagggcctg ggcgacagcg ccgcctattc ggtggacggc
     6481 gtgaaggcgc tgctggcgtc gcgccggcat cccgaccatc ccctctcccg ggagctgggc
     6541 ggcggcggcg aggtgatcga ctacaccctg gcctgcctcg tcttcgagcc cgggccgccc
     6601 gccctcatgc acctggccgc cgggccgccg agcacgacgg gcttccgcac cttcatctgc
     6661 gacagcggcg tcgcacccct ggcgaggacc ttgccgtgat cgcgtctgac cagtcgtttg
     6721 ccctcctcgc cgtgatcgcg ctgtcagccg cgttcggcta ctggatcgac agcaccccgt
     6781 ggggaaagcg gatccccggc atcctgctgg ccatcgtcat ccccatggcc ctgcgaaccg
     6841 gcgggatcct gcccggagac gcgccgctct atgacaccct gggtcgcttc ctgatcccgc
     6901 tggccgtgcc gctggtgctg ttccaggcgg actttcgccg catctaccgc ctggccgggc
     6961 cgatgctgct gatcttcgcc ctcgggacca tcgcaaccct cgcggcggtg tttctgggcg
     7021 tcgccctgtt cgacctgggc ccggacgccg cggccttcgc cgccgtgctg gcggccagcg
     7081 acatcggcgg gtcgtcgaac ctgatcgccg tgtcacagat cgtcgactgg gccagccccg
     7141 cctcgttgcc cgtggccctc gccgccgacg ccctggccgg cacgacctac tggatgatca
     7201 tcctcgcgtt gccgatggcc ccgttcctgc gccgcttctt ccggtcgccg atcatggacg
     7261 atgtcgagcg cgacaaggat gcgcccacca tgctcagcgc accctcatcg agcgcccaca
     7321 tgatggtcaa tttcggcgcc agcgcgctga tcgtcgccct gtcgaccgcg ctggccgcgc
     7381 gcctcggcgc gccgcacctg tcgatcctgg tgatttccgg catggcgctt gtggtcgcca
     7441 acctggcgcc gcagttcttc cgcaccgacc ggagcgccga ggaggcgggc atgatcctca
     7501 tctatctctt catgccggtg atcggcgctc tcaccgatct gcgctcgctg acctcgacgt
     7561 cgctcaatgt ggtcggtttc gcactgacca tcgtcctcac ccatctgctg ctgatgggcg
     7621 tcatcgcgaa gctgttcagg ttcgatatcg ccgaagccct catcgcgtcc tgcgcgggaa
     7681 tcctggggcc gccgagcact gtcgccacgg ccgcgacctt cgactggcgg gccctggtgg
     7741 ttcccggatt catgtgcgcg gttctgggca atgcgatcgc caacttcatc ggcgtggccg
     7801 tgtaccgcgc ggtccaggcg cttccctgag cagagctcgc gcgccccggg cgccccgggc
     7861 tccccgcccg ccggcggc
//
LOCUS       DAORNX010000088         7795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6906_length_7795_cov_6.540310, whole genome shotgun sequence.
ACCESSION   DAORNX010000088 DAORNX010000000
VERSION     DAORNX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_7795_cov_6.540310"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..558)
                     /locus_tag="PLO65_05550"
     CDS             complement(112..558)
                     /locus_tag="PLO65_05550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD27746.1"
                     /translation="MTEDTPPAPPEGFKPSARGPYTTHNGPFFHRLTDESFEHAFFAL
                     KRHCNSLGIVHGGMLSTFMDGVLANAVGRETGGASVTVHLSLDFLSMARAGDWVFGDA
                     RVTRRTRDLVFVEGRIRVGTREVMRAAGIFKPVNRRTDPAQSRPAP"
     gene            complement(555..1070)
                     /locus_tag="PLO65_05555"
     CDS             complement(555..1070)
                     /locus_tag="PLO65_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRD27747.1"
                     /translation="MVSKLRKAGRTVVLAAVMLTPLLAGACRAETAPPKDAPASVAAP
                     PVGTETLTIDTARGPVTFTVEIADDEAEREQGLMYRTSLGPNAGMLFEWTVPAQRAFW
                     MKNTYIPLDIIYIGFNGRILSIAAMAEPFSEEPIPSHGAALGVLEIAGGRAAELGIDI
                     GDQVHHRIFGP"
     gene            complement(1095..1712)
                     /locus_tag="PLO65_05560"
     CDS             complement(1095..1712)
                     /locus_tag="PLO65_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD27748.1"
                     /translation="MSGYDFEAQEPHEESVRINGRVKWFDAGKGYGFIVPDDPSQTGL
                     KDVLLHITSLRNAGRESALEGSVIICDVVRRTKGWQVSDIVDLDESAAPPPGERPQRR
                     FENESGSFRRDRMGGGFSGRADAPPRRTPPMRADGPLQRCTVKWFNRTKGYGFVVRDA
                     EPGDIFVHIETLRRSGLDDLQPGEGVMVRFAEGPKGLVVAEIAAS"
     gene            complement(1877..1951)
                     /locus_tag="PLO65_05565"
     tRNA            complement(1877..1951)
                     /locus_tag="PLO65_05565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1916..1918),aa:Glu,seq:ttc)
     gene            complement(2044..3846)
                     /gene="recJ"
                     /locus_tag="PLO65_05570"
     CDS             complement(2044..3846)
                     /gene="recJ"
                     /locus_tag="PLO65_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRD27749.1"
                     /translation="MADGSPEAGFLGIRRSLSGRAWRWRPADPALVRDHQLRHALSEP
                     LARALAARGVDAAGAADYLQPTLRAQFPDPSSFADMDLAARILVDAVETGRPTAVFAD
                     YDVDGASSAAQLVRWFRAMGRELAIYVPDRISEGYGPSPAAFRHLQDQGADLVVTVDC
                     GAAAHDALIAARDIGLDVVVIDHHLMRGDPPPAAALVNPNRPDDISGQGHLAAAGVTF
                     VLLAALNREARRRGLFEGRSEPDIRQWLDLAAMGAICDVTSLTGFNRAIAAQGLRTMS
                     GWANPGLKALLDVAGSQGPASTFHAGFILGPRINAGGRIGRSDLGARLLSTDDPDEAR
                     GLAEQLDALNASRKDVEKGVIDEAVLSIERDSNFDPDAPAIVVAGDGWHPGVVGIAAA
                     RLRERYRKPVVVIGVDRPSDTGKGSGRSQPGVNLGRAVQAAFDEGLLLAGGGHAMAAG
                     LTIRPSAIPDLRAFLCERLAGEMTLAAGLDAIEIDALVSPSGATRALHSEFQRLAPFG
                     PGNPEPVFAVADARVERPMMLKGGHVRCTLMDASGGRLKAVAWRAGETETGARIMAGG
                     GTMHVVGRLKPDDWQGRESVELEIEDVADPRMAL"
     gene            complement(3849..4799)
                     /gene="glpX"
                     /locus_tag="PLO65_05575"
     CDS             complement(3849..4799)
                     /gene="glpX"
                     /locus_tag="PLO65_05575"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRD27750.1"
                     /translation="MSSETLDRGLVLDAVRVTEAAAIAAWGLVGRGDEKEADQAAVDA
                     MRNALNDLAIDGEIVIGEGERDEAPMLYIGEKVGTGGPRIDIALDPLEGTTLTAKAMA
                     NALAVMAWAPAGTLLNAPDTYMDKIACGPGYAEGLIDLDASPADNARAMARAKGVDVT
                     EITVCVLDRPRHAEIIASLRSVGARVYLITDGDVAGVINTADPETGVDLYIGQGGAPE
                     GVLACAALKCVGGQFQGRLVFRNNDERSRAARWGITDLDRKYDLGEIVRADAIFAMTG
                     VTKGAMLDGVKMQGGFVHTHSLVMNSSTRTVREVRMKRPL"
     gene            complement(4853..6142)
                     /locus_tag="PLO65_05580"
     CDS             complement(4853..6142)
                     /locus_tag="PLO65_05580"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRD27751.1"
                     /translation="MTPRTWRIGVAGLGTVGGGLLQFLAERPDFAPAGGRAVITGVTA
                     RSRSRPRSIDISAIPWFDDPVALATSPDVDLFVELVGGSDGPAKAAVEAALKAGKPVV
                     TANKALIAEHGAELAVLAEAAGVPLLFEAAVMGGTPAVKMLREAMVGDDVVGVAGILN
                     GTCNFILSEMEQRGASFADVLSEAQRMGYAEADPTMDVGGFDAAHKVTILAALAFGCA
                     PNYAAAEIEGIDQVDLLDIRLAKDLGYRIRLIASADRTADGVAVRVHPSLVAQGHPLA
                     ETRGALNALFIEGTRIGRIFIQGPGAGAGPTAAAVAADIADVMTLPTRPVFQAPATGL
                     APFVAVAAARRVGKAYLRLLVKDQPGVIAAVSETLAECGVSIDSFLQKPVEDAGGVPI
                     VLTTHAIAESVLTDAINRIEKLPAVLERPRLLRIARI"
     gene            complement(6139..7359)
                     /locus_tag="PLO65_05585"
     CDS             complement(6139..7359)
                     /locus_tag="PLO65_05585"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRD27752.1"
                     /translation="MTTEFHRIRRLPPYVFEEVNRIKAKLRAEGTDIIDFGMGNPDMP
                     TPGHIVDKLIETARDPKAGRYSASKGIIGLRRAMAGYYGRRYGVKLNPDTEVVATLGS
                     KEGFANLANALTAPGDVIICPNPAYPIHAFGFIMAGGVIRHVPALSPEEYLSGIGRAV
                     KHSAPPPNILILSYPSNPTAQWVDLDFYNDAVQLARKHDLLIISDVAYSEIYFDDNPP
                     PSILQVEGAKDLAVEVNSLSKTYAMAGWRVGMVVGNERMCAALARVKSYLDYGAFLPI
                     QVAAAAALNGPQDCVAEIRGIYKSRRDTLVSSMKRAGWDIPAPPASMFAWAPIPEQYK
                     SVGSMQFSQLLVEEAGVAVAPGAGFGEYGEGYVRIGLVENEHRIRQAARNVKKFLAES
                     DRILSKAHNTVAAQ"
     gene            complement(7477..>7795)
                     /locus_tag="PLO65_05590"
     CDS             complement(7477..>7795)
                     /locus_tag="PLO65_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27753.1"
                     /translation="AQVETAIRNPGPWPTFAGIPEIPEDVRSSAAWRAAIADQQAEAE
                     ATRQAAADDTWSLTATEAFEARTRAEANPLGLHAPTDAEMAESEAYARALRKRATPPP
                     SPR"
BASE COUNT         1193 a         2685 c         2717 g         1200 t
ORIGIN      
        1 gtcgacaggc tggtccagac cttcccgttc gaccagatca acgccgcgat cgccgcccag
       61 caccacggcg agtgcgtcaa ggtggtcctg acgctgccgt aggccggcgg atcagggggc
      121 gggccgcgac tgcgccggat cggtccgcct gttgaccggc ttgaagatgc cggccgcgcg
      181 catcacctcg cgcgtcccca cccggatgcg cccctcgacg aacaccaggt cgcgcgtccg
      241 gcgcgtcacc cgcgcatcgc cgaacaccca gtcgccggcc cgggccatgg acaggaagtc
      301 cagcgacagg tgcacggtca ccgaggcgcc cccggtctcg cggccgacgg cgttggccag
      361 caccccgtcc atgaacgtcg acagcatgcc gccgtggacg atgccgaggc tgttgcagtg
      421 gcgcttgagc gcgaagaagg cgtgttcgaa cgactcgtcg gtcagacggt ggaagaacgg
      481 accgttgtgg gtggtgtagg ggccgcgcgc gctgggcttg aagccctccg gcggcgccgg
      541 aggcgtgtcc tcggtcacgg gccgaagatc cggtgatgga cctggtcgcc gatgtcgatg
      601 cccagctcgg ccgcccgtcc cccggcgatc tcgagcacgc ccagggccgc gccgtgcgag
      661 ggaatcggct cctcggaaaa gggctcggcc atcgccgcga tcgacaggat gcgaccgttg
      721 aacccgatgt agatgatgtc cagcgggatg taggtgttct tcatccagaa ggcgcgctgc
      781 gccggcacgg tccattcgaa cagcatgccg gcgttcgggc ccaggctcgt gcggtacatc
      841 agcccctgct cgcgctcggc ctcgtcgtcg gcgatctcga ccgtgaaggt caccggacca
      901 cgcgccgtat cgatggtcag ggtttcggtt ccgacgggcg gcgcggccac gctcgcgggc
      961 gcgtctttcg gcggcgccgt ttccgcccgg caggcgccgg ccagcaacgg ggtgagcatc
     1021 accgcggcca ggacaaccgt ccgaccggcc ttgcgcaact tcgataccac gggcgactcc
     1081 aggtgcgtcg ggcgtcagga cgccgcgatt tcagccacga ccagaccctt gggcccctcc
     1141 gcgaaacgca ccatcacgcc ttcgccgggc tgcaggtcat caaggccgct gcgacgcagc
     1201 gtctcgatgt gcacgaagat atcccccggc tcggcgtcgc gcaccacgaa accgtagccc
     1261 ttggtgcggt tgaaccattt gaccgtacag cgttgcaacg gcccgtcggc ccgcatcggc
     1321 ggggtccgtc ggggcggcgc atcggcgcgg cccgaaaagc cgccgcccat ccggtcgcgt
     1381 cgaaagctcc cgctttcgtt ttcaaaacgc cgttgcggcc gctcgcccgg cggcggcgcc
     1441 gcgctttcgt cgagatcgac aatgtcggac acctgccagc ccttggtccg gcgcacgacg
     1501 tcgcagatga tcaccgaacc ttcgagcgcc gattcccgac cggcgtttcg cagcgaagtt
     1561 atgtgcagga gcacatcctt caacccggtc tggctggggt cgtcgggaac aatgaacccg
     1621 tagcccttcc cagcatcaaa ccacttcaca cggccattga tccgcacgga ttcttcgtgc
     1681 ggctcctggg cctcgaaatc ataaccagac attccgcccc tctgccccgc cctaaagtcc
     1741 tttctggacg agataacaag tataacactg ttctcgtgga aggagagccg tcaatgaccg
     1801 aattcgcgca ggcgatgact cgggccagcg ccgcgacatt tggtcgcaat tcgggtgggg
     1861 cagtcactcg cggcgatggc acgccctagg ggagtcgaac ccctcttacc agattgaaaa
     1921 tctggggtcc taaccgatag acgaagggcg cccggtcgcg aggaagcggc tgtatattcg
     1981 cgactcccgc cacgcgcaac cccccacgcg gacgagaatt gggcccggga caaggttgtc
     2041 cgatcagagc gccatccggg ggtcggcgac gtcctcgatt tccagctcga cgctctcccg
     2101 cccttgccag tcgtcgggct tcagccggcc gacgacatgc atcgtcccgc ccccggccat
     2161 gatccgcgcg cccgtttccg tttcaccggc tcgccaggcg accgccttca ggcgtccgcc
     2221 ggaggcatcc atgagggtgc agcgcacatg gccgcccttg agcatcatcg gccgctcgac
     2281 gcgcgcatcg gcgacggcga acaccggctc gggattgccc ggtccgaacg gcgccaggcg
     2341 ctggaactcg ctgtgcagcg cgcgcgtggc cccggaaggg ctgaccaggg cgtcgatctc
     2401 gatggcgtcc agcccggccg cgagggtcat ctccccggcc agccgttcgc agaggaaggc
     2461 gcgcagatcg ggaatggccg aggggcgaat ggtcagtccg gcggccatgg cgtgcccgcc
     2521 accggccagc agcaggccct cgtcgaacgc cgcctgcacc gctcggccca ggttgacgcc
     2581 gggctgggag cggcccgagc ccttgcccgt gtccgacggc cggtcgacgc cgatgacgac
     2641 caccggcttg cggtagcgct cgcgcaggcg ggccgcggcg atgcccacca cgcccggatg
     2701 ccaaccgtca ccggccacca cgatggccgg cgcgtcgggg tcgaagttgc tgtcacgctc
     2761 gatggacagc acagcctcgt cgatcacgcc cttctcgacg tccttgcgcg aggcgttgag
     2821 cgcatcgagc tgttcggcca gccccctcgc ctcgtccgga tcgtcggtcg acagcagccg
     2881 cgcgcccagg tccgaccggc cgatgcggcc gccggcgttg attctcggtc cgaggatgaa
     2941 gccggcgtgg aacgtcgagg cagggccctg cgagccggcc acgtccagca gggccttgag
     3001 accgggattg gcccagcccg acatggtccg caggccctgg gcggcgatcg cccggttgaa
     3061 cccggtgagg ctggtcacgt cacatatggc gcccatggcc gccagatcga gccactggcg
     3121 gatgtcgggt tcgctccgcc cctcgaacag gccgcgccgc cgggcctcgc gatttagcgc
     3181 cgcaagcagc acgaaggtta cgccggcggc ggccagatgg ccctggccgg agatgtcgtc
     3241 cgggcggttg ggattgacca gggccgcggc cggcggtggg tcgccgcgca tcaggtgatg
     3301 atcgatgacc accacgtcca gcccgatgtc gcgggcggcg atcagggcgt cgtgggcggc
     3361 ggcgccacaa tcgaccgtga ccaccaggtc ggcgccctgg tcctgcagat ggcggaaggc
     3421 ggccgggctg ggcccgtagc cctcgctgat ccggtcgggg acgtagatcg ccagctcgcg
     3481 gcccatggcg cggaaccagc gcaccaactg ggcggcgctg gacgccccgt cgacgtcata
     3541 gtctgcgaat accgccgtcg gccgtccggt ctcgaccgcg tccaccagga tgcgggcggc
     3601 caggtccatg tcggcgaagc tcgagggatc gggaaactgg gcccggagcg tcggctgcag
     3661 atagtcggcc gccccggcgg cgtcgacgcc gcgggcggca agggcgcggg ccaggggttc
     3721 gctgagcgcg tggcgaagct ggtggtcgcg caccagcgcc gggtcggccg gtcgccagcg
     3781 ccacgcccgc cccgacaggg agcgacgaat gccgaggaag cctgcctcgg gcgatccgtc
     3841 ggccatgatc agagcggccg cttcatccgc acttcacgca ccgtgcgggt ggaggagttc
     3901 atgaccagcg agtgggtgtg gacgaatccg ccctgcatct tcacgccgtc gagcatggcg
     3961 cccttggtca cgccggtcat ggcgaagatc gcgtcggccc ggacgatctc gcccaggtcg
     4021 tatttgcggt ccaggtcggt gatgccccag cgggccgccc ggctgcgctc gtcgttgttg
     4081 cggaacacca gacggccctg gaactgaccg ccgacgcact tcagcgccgc gcaggccagc
     4141 acgccctcgg gcgcgccgcc ctggccgatg taaaggtcga caccggtctc gggatcggcc
     4201 gtgttgatca ccccggccac atcgccatcg gtgatcagat agacgcgcgc gccgaccgac
     4261 cgcaggctgg caatgatctc ggcgtggcgt ggccggtcca gcacgcagac cgtgatctcg
     4321 gtcacatcga cgcccttggc cctggccatg gcccgggcgt tgtcggcagg gctggcgtcg
     4381 aggtcgatca ggccctcggc gtagccgggg ccgcaggcga tcttgtccat ataggtgtcg
     4441 ggggcgttca gcagcgtgcc cgccggagcc caggccatca cggccagggc gttggccatg
     4501 gccttggcgg tcagggtcgt gccttcgagg ggatcgagcg cgatgtcgat gcgcggacca
     4561 ccggtgccga ccttctcacc gatgtagagc atcggggcct catcccgctc gccctcgccg
     4621 atgacgatct cgccgtcgat agccagatcg ttgagggcgt tgcgcatggc gtcgaccgcg
     4681 gcctggtcgg cttccttctc gtcgccccgg cccaccaggc cccaggcggc gatggcggcc
     4741 gcttcggtca cccggaccgc gtcaaggacc aggccccggt ccagcgtctc agaactcatg
     4801 aaagtcagtc tcccggcccg tgcagggcgt tctggcgatt gtcggcggat tctcaaatgc
     4861 gcgcgatgcg caacagccgc ggacgctcta gcacggcggg gagcttttcg atgcggttta
     4921 tcgcatcggt cagcaccgac tcggcgatgg cgtgggtcgt cagcacgatc ggcacgccgc
     4981 cggcgtcctc gaccggcttc tgcagaaaac tgtcgatcga cacgccgcat tcggcgaggg
     5041 tttccgacac cgcggcgatg actccgggct gatccttcac cagcaggcgc aggtaggcct
     5101 tgccgacgcg ccgggcggcc gccacggcga cgaacggcgc gagtccggtc gccggggcct
     5161 ggaacacggg ccgggtcggc agggtcatga catcggcgat gtcggcagcc acggcggccg
     5221 cggtcggtcc cgcgcccgcg ccgggcccct ggatgaagat ccggccgatc cgcgtgccct
     5281 cgatgaacag cgcgttgagc gcgccgcgcg tctcggccag cggatggccc tgggcgacca
     5341 gcgagggatg cacgcgaacg gccacgccgt cggcggtcct gtcggccgag gcgatcagcc
     5401 ggatgcgata gccaaggtcc ttggccagcc ggatgtcgag cagatcgacc tggtcgatgc
     5461 cctcgatctc ggcggcggcg tagttgggcg cgcagccgaa cgccagggcg gccaggatgg
     5521 tcaccttgtg ggccgcgtcg aagccgccca cgtccatggt cgggtcggcc tcggcgtaac
     5581 ccatgcgttg ggcctcgctg agcacgtcgg cgaacgaggc gccgcgttgt tccatctcgc
     5641 tgaggatgaa attgcaggtg ccgttgagaa tgccggccac gccgaccacg tcgtcgccga
     5701 ccatggcctc gcgcagcatc ttcaccgccg gcgtcccgcc catcaccgcc gcctcgaaca
     5761 gcaacggcac gccggccgcc tcggccagaa cggcgagttc cgcgccatgt tcggcgatca
     5821 aggccttgtt ggccgtgacc acgggcttgc cggccttcag cgcggcctcg acggcggctt
     5881 tggccgggcc gtcagagccg ccgaccagct cgacgaacag gtcgacgtca ggagacgtcg
     5941 ccagggccac cggatcgtcg aaccagggga tggccgagat gtcgatgctg cgcggccgcg
     6001 agcgtgaacg ggccgtcacg ccggtgatca ccgcccgacc cccggccggc gcgaagtcgg
     6061 gccgctcggc gaggaactgc aacaggccgc caccgacggt gcccaggccg gcgacaccga
     6121 tacgccaggt gcgcggggtc attgggcggc cacggtgttg tgggccttgg acaggatgcg
     6181 gtcggactcg gcgaggaact tcttcacgtt gcgggccgcc tggcggatgc ggtgctcgtt
     6241 ctcgacaagg ccgatgcgca catagccctc gccgtattcg ccaaagcccg cgcccggagc
     6301 cacggcgacc ccggcctcct ccaccagcag ttgggagaac tgcatcgagc cgaccgactt
     6361 gtactgctcg gggatcgggg cccaggcgaa catcgaggcc ggcggcgcgg gaatgtccca
     6421 gccggcccgc ttcatgctgc tgaccagggt gtcgcggcgg ctcttgtaga tgccgcggat
     6481 ctccgcgacg caatcctgcg ggccgttcag ggccgccgcg gcggccacct ggatcggcag
     6541 gaaggcgccg tagtcgaggt aggacttcac tcgcgccaaa gccgcgcaca tgcgttcatt
     6601 gccgaccacc atgcccacgc gccagcctgc catggcgtag gtcttcgaca ggctgttgac
     6661 ctcgacggcc aggtccttgg ccccctcgac ctgcaggatc gatggcggcg ggttgtcatc
     6721 gaaatagatc tccgaatagg cgacatcgga aatgatcagc aggtcgtgct tccgcgccag
     6781 ctgcacggcg tcattgtaga agtccagatc gacccactgg gccgtcgggt tggacggata
     6841 gctgaggatc aggatgttgg gcggcggcgc cgagtgcttc accgcccggc cgatacccga
     6901 caggtactcc tccggagaca gggccggcac atggcggatt acgccgccag ccatgatgaa
     6961 gccgaaggca tggatcggat aggccgggtt ggggcagata atcacgtcgc ccggcgccgt
     7021 cagggcgttg gcgaggttgg cgaacccctc cttcgacccg agcgtggcca cgacctcggt
     7081 gtcggggttc agcttcacgc catagcggcg gccgtagtag ccggccatgg cgcggcgaag
     7141 gccgatgatt cccttggagg cggaatagcg tccggccttg ggatcgcggg cggtttcgat
     7201 cagcttgtcg acgatgtggc cgggcgtggg catgtcggga ttgcccatgc cgaagtcgat
     7261 gatgtccgta ccctcggccc gcagcttggc cttgatccgg ttcacttcct cgaagacgta
     7321 gggcggcaga cgccggatac ggtggaattc ggtggtcata aatgggctct ctgcgccgtt
     7381 cgcggcgcgc tgggagcgaa tgtcgcgcat ggataacccc cgggaggggc gtctccaagc
     7441 aggttcgggg aaataaatgc ctccgccgcg ccggatttac ctcggcgacg gaggcggggt
     7501 agctcgcttg cgcagggcgc gggcataggc ctccgactct gccatttcgg cgtcggtcgg
     7561 tgcgtgcagg cccagggggt tggcctcggc gcgcgtccgg gcctcgaaag cctccgtggc
     7621 cgtcaggctc caggtgtcgt cggcggccgc ctgccgggtc gcctcggcct cggcctgctg
     7681 gtcggcgatg gccgcgcgcc aggcggccga ggagcgcaca tcctccggga tttcgggaat
     7741 gccggcgaaa gtcggccagg gtccgggatt gcggatcgcc gtctcgacct gggcc
//
LOCUS       DAORNX010000089         7770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6940_length_7770_cov_6.470642, whole genome shotgun sequence.
ACCESSION   DAORNX010000089 DAORNX010000000
VERSION     DAORNX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_7770_cov_6.470642"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..980
                     /locus_tag="PLO65_05595"
     CDS             30..980
                     /locus_tag="PLO65_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRD27754.1"
                     /translation="MSAFDAVAVGRRVLATEAEALQTLADQLGPPFVRAVEILVACKG
                     RIVCTGMGKSGHVARKIAATLASTGAPAMFVHPAEASHGDLGMIGQVVLALSKSGDVR
                     ELADVIAYAHRFAIPLIAMTANEGSQLGLAASLVLALPDAPEATGEVRAPTTSTTLQI
                     ALGDALAVALLERRGFTARDFHVFHPGGKLGAMLRTVGDLMHRLEEAPLARADITMPE
                     AIRVMSERRLGIVGVTDDDGALIGVITDGDLRRHFDGLADKTVGQVMTRNPKSVTAET
                     LAGDAARLMNDSRITVLFVVRDGKPVGVLHVHDVLSAGVV"
     gene            complement(1173..1742)
                     /locus_tag="PLO65_05600"
     CDS             complement(1173..1742)
                     /locus_tag="PLO65_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27755.1"
                     /translation="MKSLITAAVVAASLAAAPAWAADFQPAPNADYVLDLDTPDDSFS
                     LWRQSDLSGISAARAQVTFRRVGRKKAFSPGASIILANDQSEARLSFVVAPKGAVAIL
                     IASRGETKLGSELFVMPVEPGETFGLELDWTPEGKVVVRMATRAVKTLGGDGFERHEI
                     TMAGAPTRMEIVGVAAEIELKPLTLGSVR"
     gene            1812..3314
                     /locus_tag="PLO65_05605"
     CDS             1812..3314
                     /locus_tag="PLO65_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRD27756.1"
                     /translation="MPFLPRADLVPNTAAYENEPLVKATGFREYDARWLFGPDINLLG
                     VQALGLGLGTYIHELGQSKIVVGHDFRSYSASIKHALVIGLLEAGCQVHDIGLALSPT
                     AYYAQFDLDAPCVAMVTASHNENGWTGVKMGAARPLTFGPEEMGRLKEIVLAGAFVRR
                     DGGRLVREEGVAERFIADVARRVSITRPLKVVAACGNGTAGAFVPQALRDMGVAVVIE
                     MDCELDWTFPKYNPNPEDQEMLHAMARAVREHGADLALGFDGDGDRCGVVDDTGEEIY
                     ADKIGLMLARDLAPLNPGATFIVDVKSTGLYATDPVLAANGCTTVYWKTGHSYIKRKT
                     AELGALAGFEKSGHFFLNEPLGRGYDCGLTAAAAILGMLDRNPDKTLSDMRKALPVAW
                     TSLTMSPHCADEVKYDVVAAVVKEYEALAAAGGKILGRAISEVITVNGVRVALEDGSW
                     VLVRASSNKPEIVVVVESTQSDADMRALFREEVKPRLAKHPQVGAYNQEV"
     gene            3328..4629
                     /locus_tag="PLO65_05610"
     CDS             3328..4629
                     /locus_tag="PLO65_05610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRD27757.1"
                     /translation="MRVAMIGTGYVGLVSGACFADFGHDVCCIDKDAGKIARLERGEI
                     PIFEPGLDDLVARNVRAGRLSFAVEADQAVRDADAVFIAVGTPSRRGDGHADLSYVHA
                     AAEEIAHLMDGFTVVVTKSTVPVGTGDEIEAIIRKASPNADFAVVSNPEFLREGAAIE
                     DFKRPDRVVVGTEDERAKAVMNELYRPLFLNETPIVFTGRRTSELIKYAANAFLALKI
                     TFINEIADLCEAVGADVQQVARGIGLDKRIGAKFLNAGPGYGGSCFPKDTLALVRTAQ
                     DARTPVRLIETTVSINDARKKAMAGRVADVLDGDLTGKVIGVLGLTFKPNTDDMRDAP
                     SLDIVPALQAMGATVQAYDPEGHEARQLLRDVAFKDGPYEAVEGADAVVIITEWDQFR
                     ALDLDRMKLLMRAPNVVDLRNIYRPDDMRARGFRYSSVGRS"
     gene            4852..5520
                     /locus_tag="PLO65_05615"
     CDS             4852..5520
                     /locus_tag="PLO65_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HRD27758.1"
                     /translation="MWVLLGVAVVVAGFAARLNPLLIVATAAVVTGIAAGLDLVATLA
                     VLGKAFRDNRYISVVWLILPLIGLLEREGLQERAKALIGRLRSATAGRLLLIYMVIRQ
                     GTAAIGLTSLGGHAQMVRPLIAPMTEAAAETQAPDLAPPLRERVKAMAAATDNIGLFF
                     GEDIFIAIASILLIKGFLEENGIIVEPLHLSMWAIPTAVAALLIHGARLLLLDRRLAR
                     EAGR"
     gene            5517..6467
                     /locus_tag="PLO65_05620"
     CDS             5517..6467
                     /locus_tag="PLO65_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HRD27759.1"
                     /translation="MITLDVLYVLAGLLFAIYAVATVLDATNPKRFGSAAFWALFAAS
                     FLFGSYLPDVANGGILVAMALLAGFTLLGRGDHPQPVDKAQRAARWGNRLFIPALIPP
                     VVTLAGTLTLKGVAVGGQPLVDPKQVTLISLGAGIVLATAVALVMFRASPAGATREGR
                     RLMESVGWAGLLPQALAALGAVFAAGHVGDVIGELATTWLPLGAPLAAVVAYTVGMAL
                     FTAIMGNAFAAFPVMTAAIGLPIIVGKFGGDPAIMGAIGMLSGFCGTLTTPMAANFNI
                     VPAALLELTDRYGVIKAQLPTALLLLLINTVLMYVLVYRF"
     gene            6445..7464
                     /locus_tag="PLO65_05625"
     CDS             6445..7464
                     /locus_tag="PLO65_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HRD27760.1"
                     /translation="MSSSIASDARLTADLAERFAAIALGHVTREFPHKADHVFTGLQD
                     IGPHRTHHPIFYGSFDWHSCVHGYWTLATLLRLFPDTPSAPAIRGLFANSLTPSKVAV
                     ELDYLSRPMAAGFERPYGWAWLLKLAAELRAHDDPAAFNALAPLARAFSDRFKAFLPK
                     ATYPLRAGVHSNTAFALRLAMDYAESCGDPGLADLARATAIGWYAGDVDCQAWEPSGD
                     EFLSSALMEAECLRRMLSPDAFSAWFSAFLPRTAGRQPATLFSPATVSDRSDGKIAHL
                     DGLNLSRAWCWRGIASALAADDPARTVALEAAESHLASALPHVAGDYMGEHWLASFAL
                     LAMLE"
     gene            7547..>7770
                     /locus_tag="PLO65_05630"
     CDS             7547..>7770
                     /locus_tag="PLO65_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRD27761.1"
                     /translation="MPAPVVALETTHHVRDTCLCLHAQRAARVLARRFDDALRPVGLT
                     NGQFSLLMALNGQEPAPMGRIAALLGADRTT"
BASE COUNT         1128 a         2779 c         2667 g         1196 t
ORIGIN      
        1 ccggccgccg ccgcagcgga cgccgcgaag tgagcgcctt cgacgccgtc gcggtcggtc
       61 gccgggtgct ggccaccgag gccgaggcgc tgcagaccct ggccgaccag ctgggcccgc
      121 cgttcgtccg cgccgtcgag atcctcgtcg cctgcaaggg ccgcatcgtc tgcaccggca
      181 tgggcaagag cggccatgtg gcgcgcaaga tcgccgccac cctggcctcg accggcgcgc
      241 cggcgatgtt cgtccatccg gccgaggcca gccatggcga cctgggcatg atcggccagg
      301 tggtgctggc gctgagcaag tccggcgacg tgcgcgagct ggccgacgtg atcgcctacg
      361 cccatcgctt cgccatcccg ctgatcgcca tgacggccaa cgagggcagc cagctgggcc
      421 tcgccgcgag cctggtgctg gcgctgcccg atgcacccga ggcgaccggc gaggtgcgcg
      481 ccccgaccac ctcgacgacc ctgcagatcg ccctcggcga cgccctggcc gtggccctgc
      541 tcgagcggcg cggcttcacg gcgcgggact tccacgtctt ccaccccggc ggcaaactgg
      601 gggccatgct gcgcacggtc ggcgacctga tgcaccggct cgaggaagcg ccgctggccc
      661 gcgccgacat caccatgccc gaggcgatcc gggtgatgag cgagcggcgg ctgggcatcg
      721 tcggggtcac cgacgacgac ggcgccctga tcggggtgat caccgacggc gacctgcgcc
      781 gccacttcga cggcctggcc gacaagaccg tcggccaggt gatgacccgc aatcccaagt
      841 cggtcaccgc ggagaccctc gccggggacg ccgcccggct gatgaacgac agccgcatca
      901 ccgtgttgtt cgtggtcagg gatggcaagc cggtgggcgt gctgcacgtc cacgacgtgc
      961 tgagcgccgg ggtggtctga cccggccccc agggcccatc gacactcagc gatcgagagg
     1021 caatcaaacc cagcctgtcc ccgcgaaggc ggggacccag gccattgtgg atgcttcacc
     1081 ggcgcttcga gccttgaaac tccgcctggg tccccgcctt cgcgggggca atcgggagaa
     1141 ggacagcccc agcccctgaa tgtcgattcc gcctagcgga ccgagcccag ggtcagtggt
     1201 ttcagttcga tttcggcggc gacgcccacg atctccatcc gggtcggcgc gccggccatg
     1261 gtgatctcgt gccgctcgaa gccgtcgccg cccaacgtct tgacggccct ggtcgccatc
     1321 ctgacaacga ccttgccctc gggcgtccag tcgagctcca ggccgaaggt ctcgcccggc
     1381 tcaaccggca tcacgaacag ctcactgccg agtttggtct cgccgcggga agcgatcagg
     1441 atcgcgaccg caccctttgg cgccacgaca aaggacagcc gcgcctcgct ctggtcgttg
     1501 gcgaggatga tcgaggcgcc ggggctgaag gcctttttcc tgccgacccg ccggaaggtg
     1561 acctgcgcgc gcgccgcgct gatccctgac aggtcgctct gccgccagag cgagaagctg
     1621 tcgtccggcg tgtcgaggtc gaggacatag tcagcgttcg gggccggctg gaagtccgcc
     1681 gcccaggccg gggccgcggc caggctcgcc gcgaccacgg cggccgtgat cagcgatttc
     1741 ataactcccc cctcaccaag tcgtcactcg gacgttatag ggggcgcctt cgtcacgcga
     1801 gtaaaccctc gatgcccttc cttccccgcg ccgatctcgt ccccaacacc gccgcctatg
     1861 agaacgagcc gctcgtcaag gcgacgggct ttcgcgaata cgacgcgcgc tggctgttcg
     1921 ggccggacat caacctgctg ggcgtgcagg cgctcggcct ggggctgggg acctacatcc
     1981 acgagctggg ccagtcgaag atcgtcgtcg gccacgactt ccggtcctat tcggcctcga
     2041 tcaagcacgc cctggtcatc ggcctgctgg aagcgggctg ccaggtgcat gacatcggcc
     2101 tggccctgtc gccgacggcc tactacgccc agttcgacct cgacgcgccc tgcgtcgcca
     2161 tggtcaccgc cagccacaac gagaacggct ggaccggggt gaagatgggc gcggcgcggc
     2221 cgctgacctt cggtcccgag gagatgggcc ggctgaagga gatcgtcctg gccggcgcct
     2281 ttgtccgccg cgacggcggc aggctggtcc gcgaggaggg cgtggcggag cggttcatcg
     2341 ccgatgtcgc ccggcgcgtc tcgatcaccc ggccgctgaa ggtggtcgcc gcctgcggca
     2401 acggcacggc cggcgccttc gtgccccagg ccctgcgcga catgggcgtg gccgtggtca
     2461 tcgagatgga ctgcgagctc gactggacct tcccgaagta caatcccaat cccgaggacc
     2521 aggagatgct gcacgccatg gccagggccg tgcgcgagca tggcgcggac ctggccctgg
     2581 gcttcgacgg cgacggcgac cgctgtggcg tggtcgacga cacgggcgag gagatctacg
     2641 ccgacaagat cggcctgatg ctggcccggg atctcgctcc gctgaacccc ggcgcgacct
     2701 tcatcgtcga cgtgaagtcg accggcctct acgccaccga cccggtgctg gcggccaacg
     2761 gctgcaccac cgtctactgg aagaccggcc acagctatat caagcgcaag accgccgagc
     2821 tgggcgccct ggccgggttc gagaagagcg gccacttctt cctcaacgag ccgctgggcc
     2881 ggggctacga ctgcggcctg acggcggcgg ccgcgatcct cggcatgctc gaccgcaacc
     2941 cggacaagac gctttcggac atgcgcaagg cgctgccggt cgcctggacc tccctgacca
     3001 tgagcccgca ctgcgccgac gaggtgaagt acgacgtggt cgccgcggtg gtgaaggaat
     3061 acgaggccct ggccgccgcc ggcggcaaga tcctcggccg ggcgatcagc gaggtgatca
     3121 ccgtcaacgg cgtgcgcgtg gcgctggagg acggcagctg ggtgctggtc cgcgcctcgt
     3181 ccaacaagcc cgagatcgtc gtggtcgtcg aaagcacgca gtcggacgcc gacatgcgcg
     3241 cgctgttccg cgaggaggtg aagcccagac tggcgaaaca cccccaagtc ggggcataca
     3301 accaggaagt ctgaggaatc ctgatccatg cgcgtcgcca tgatcggcac cggctatgtc
     3361 ggcctggtgt ccggggcctg cttcgccgat ttcggccatg acgtctgctg catcgacaag
     3421 gatgccggca agatcgcgcg gctcgagcgc ggcgagatcc cgatcttcga gcccgggctc
     3481 gacgacctgg tcgcgcgcaa cgtgcgggcc ggccggctga gcttcgccgt cgaggcggac
     3541 caggccgtgc gcgacgccga cgcggtgttc atcgccgtgg gcaccccctc gcgacgcggc
     3601 gacggccatg ccgacctctc ctacgtccac gccgcggccg aggagatcgc ccatctgatg
     3661 gacggcttca ccgtggtggt gaccaagtcg accgtgccgg tgggcaccgg cgacgagatc
     3721 gaggcgatca tccgcaaggc cagccccaac gccgacttcg cggtggtgtc caatcccgag
     3781 ttcctgcgcg agggcgcggc gatcgaggac ttcaagcggc ccgaccgggt ggtcgtgggg
     3841 accgaggacg agcgcgcgaa ggccgtgatg aacgagctct accggccgct gttcctcaac
     3901 gagacgccga tcgtgttcac cggccggcgc accagcgagc taatcaagta cgcggccaac
     3961 gccttcctgg cgctgaagat caccttcatc aacgagatcg ccgacctgtg cgaggctgtc
     4021 ggcgccgacg tgcagcaggt ggcgcgcggc atcggcctgg acaagcgcat cggggccaag
     4081 ttcctcaacg ccggccccgg ctacggcggc agctgctttc ccaaggacac cctggccctt
     4141 gtgcgcacgg cccaggacgc caggaccccg gtgcggctga tcgagaccac cgtgtcgatc
     4201 aacgacgcgc gcaagaaggc catggccggg cgcgtcgcgg atgtgctcga cggcgacctg
     4261 accggcaagg tgatcggggt gctgggcctg accttcaagc ccaacaccga cgacatgcgc
     4321 gacgcgccga gcctcgacat cgtgccggcc ctgcaggcca tgggcgcgac cgtccaggcc
     4381 tatgaccccg agggccacga ggcccgccag ctgctgcgcg acgtggcctt caaggacggg
     4441 ccctacgagg ccgtcgaggg cgccgacgcg gtggtcatca tcaccgagtg ggaccagttc
     4501 cgcgccctcg acctggaccg gatgaagctg ctcatgcgcg cgcccaacgt ggtcgacttg
     4561 cgcaacatct accgccccga cgacatgcgg gcgcgcgggt tcaggtattc gagcgtcggg
     4621 cgttcctgag gccttcctct cccgattggg agagggggac cagccgaagg ctggtggtga
     4681 gggaggctcc ggggtgagcc gggacgtccg gatcgaccgc gcatgaccct gcaccaccgc
     4741 gcctccccct ccaccatgct ccgcatggtc cccctccccc aggtggggga ggattggcgt
     4801 tgagggtgat tccgggtcca ctctgcccgt gagacagagg agtatcgggc catgtgggtc
     4861 ttgctggggg tggcggtcgt cgtcgcgggg ttcgcggccc ggctcaatcc gttgttgatc
     4921 gtcgcgacgg ccgctgtcgt gacgggcatc gcggcggggc tggacttggt cgccaccctg
     4981 gcggtgctgg gcaaggcgtt ccgggacaac cgctacatca gcgtggtctg gctgatcctg
     5041 ccgctgatcg ggctgctcga gcgggaaggg ctgcaggagc gggccaaggc gctgatcggc
     5101 cggctcaggt cggccaccgc cgggcggctg ctgctgatct acatggtcat tcgccagggt
     5161 acggcggcga tcggcctgac ctcgctgggc ggccacgccc agatggtccg cccactgatc
     5221 gccccgatga ccgaggcggc cgccgagacc caggcccccg acctcgctcc gcccctgcgc
     5281 gagcgggtca aggccatggc cgccgccacc gacaacatcg gcctgttctt cggcgaggac
     5341 atcttcatcg ccatcgcctc gatcctgctg atcaagggct tcctcgagga gaacggcatc
     5401 atcgtcgagc cgctgcatct gtcgatgtgg gccatcccga cggccgtcgc ggccctgctg
     5461 atccacggcg cgcggttgct gctgctcgac cgccggctgg cccgggaggc cggccggtga
     5521 tcacgctcga tgtcctctat gtgctggccg gcctgctgtt cgcgatctac gccgtcgcga
     5581 cggtgctgga cgcgaccaac ccgaagcgct tcggctcggc ggccttctgg gcgctgttcg
     5641 cggccagctt cctcttcggc tcctacctgc ccgacgtggc caacggcggc atcctcgtgg
     5701 ccatggccct gctggccgga ttcaccctgc tgggccgggg cgatcatccc cagcccgtcg
     5761 acaaggcgca gcgcgcggcc cgctggggca atcgcctgtt cattccagcc ctgatcccgc
     5821 cggtggtgac gttggccggg acgctgacgc tgaagggggt ggcggtcggc ggccagccgc
     5881 tggtcgatcc caagcaggtc acgctgatct ccctcggcgc cggcatcgtg ctggccaccg
     5941 ccgtggccct ggtcatgttc cgcgccagcc ccgccggcgc cacgcgcgag ggccgccggc
     6001 tgatggagtc ggtcggctgg gccgggctcc tgccgcaagc cctcgccgcg ctgggagcgg
     6061 tgttcgccgc cggccatgtc ggcgacgtga tcggcgagct ggccacgacc tggctgccgc
     6121 tgggcgcgcc gctggccgcc gtggtcgcct acacggtcgg catggccctg ttcacggcca
     6181 tcatgggcaa cgccttcgcc gcctttccgg tgatgaccgc ggccatcggt ctgccgatca
     6241 tcgtggggaa attcggcggc gatccggcga tcatgggcgc gatcggcatg ctgtcgggct
     6301 tctgcggcac cctgaccacc ccgatggcgg ccaatttcaa cattgtcccg gcggcgctgc
     6361 tggagctgac cgaccgctac ggcgtgatca aggcccagct gccgaccgcc ctcctgctgc
     6421 tgctgatcaa cacggtgctg atgtatgtcc tcgtctatcg cttctgacgc ccgtctgacg
     6481 gcggacctgg ccgagcggtt cgccgccatc gcgctgggcc acgtcacccg cgagttcccg
     6541 cacaaggccg accatgtgtt caccggcctg caggacatcg gcccgcaccg gacccatcat
     6601 ccgatcttct acggcagctt cgactggcac agctgcgtgc acggctactg gaccctggcg
     6661 accctgctgc ggctgttccc cgacacgccg agcgcgccgg cgatccgcgg cctgttcgcg
     6721 aacagcctga cgccgtcgaa ggtcgccgtg gagctggact acctgtcgcg gccgatggcc
     6781 gccgggttcg agcgccccta cggctgggcc tggctgctga agctggcggc cgagctgcgg
     6841 gcgcatgacg atccggcggc gttcaacgcc ctcgcgccgc tggccagggc cttttcagac
     6901 cggttcaagg cctttctgcc caaggccacc tatccgctgc gggccggggt gcattcgaac
     6961 accgccttcg cgctgcggct ggcgatggac tacgccgaga gctgcggcga tccggggctg
     7021 gccgacctgg cccgcgcgac cgccatcggc tggtacgccg gggacgtcga ctgccaggcc
     7081 tgggaaccga gcggcgacga gttcctgtcg tcggccctga tggaggccga gtgcctgagg
     7141 cggatgctgt cccccgacgc gttcagcgcc tggttctccg ccttcctgcc gcgcaccgcg
     7201 ggccgccagc cggcgaccct gttcagcccg gccaccgtca gcgatcgcag cgacggaaag
     7261 atcgcccatc tcgacggcct gaacctcagc cgcgcctggt gctggcgggg aatcgcctcc
     7321 gcgctcgccg cggacgaccc ggcccggacg gtcgcgctgg aggcggccga gtcccacctg
     7381 gccagcgccc tgccccacgt ggccggcgac tacatgggcg agcactggct ggccagtttc
     7441 gccctgctgg cgatgctgga ataggggaca cgttcctgcg gtagatgtcc ccgtcagccc
     7501 gcctggctgg cttgactcta tgggttgata tcaacataac tacgccatgc ccgcgcccgt
     7561 cgtcgccctc gagaccaccc accacgtccg tgacacctgc ctgtgcctgc atgcgcagcg
     7621 cgccgcccgg gtgctggcgc ggcggttcga cgacgccctg cggccggtcg ggctgaccaa
     7681 cggccagttc tcgttgctga tggcgctgaa cggtcaggag cccgcgccga tggggcggat
     7741 cgccgccctg ttgggcgcgg accggaccac
//
LOCUS       DAORNX010000090         7729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6985_length_7729_cov_8.888976, whole genome shotgun sequence.
ACCESSION   DAORNX010000090 DAORNX010000000
VERSION     DAORNX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6985_length_7729_cov_8.888976"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLO65_05635"
     CDS             complement(<1..1037)
                     /locus_tag="PLO65_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD27762.1"
                     /translation="MIFDRPVPRRTPWLIEARALMVLAAPMAGSQLAQMAIMTTDVVL
                     LGRLSREALAAAAIGNTVFYFAWLIGSGPGMAVSPVVAQILGAWPGDRARVRATVRMG
                     LWVTMLAALPLMLVMLNARWILSHLGQDPELARGAGVFVSMLAIGLPFALGYQALRSF
                     TAALGKPTAALWVMLAAIGVNGFLGYGLIFGHFGLPKLGLLGSGMATAISSIFEFAAM
                     VVVIWLTPSLRAYRIFRRAHRPVPVRVRELIRLGMPISMTMIFEAMLFNTMTLVMGTF
                     GAIAVAAHQIALNFASITFMVPLGIGMASTVRVGLAAGRGDLAGARRAGVTAMVLAVG
                     FISVVAVAMVL"
     gene            complement(1353..2606)
                     /locus_tag="PLO65_05640"
     CDS             complement(1353..2606)
                     /locus_tag="PLO65_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD27763.1"
                     /translation="MSDGSVDLKQTAAEHAWSMPLSEIKVADPLLFQTDTMWPYFERL
                     RKEAPVHYQAEHEFGPYWSVTRYNDIMAVDTNHQVFSSEGGITLAEGSEDFRLPMFIA
                     MDPPKHDVQRKTVSPVVSPGNLQYLEPIIRERAAKILDDLPIGEEIDWVDKVSIELTT
                     MTLATLFDFPWEDRRKLTYWSDTATASKFSPFYKGEDHRRAALFECVEYFTRLWNERV
                     NAPPKNDLISMLCHGESTRNMDRMEYLGNLILLIVGGNDTTRNTITGSILALNQNPDQ
                     FAKLKADPSLIPSMVSETIRWQTPLAYMRRRALQDIELQGQKIRKGDKVLMWYVSGNR
                     DDAVIENPNSYIIDRERPRQHLSFGFGIHRCVGNRLAELQLRIIWEEILARFPDIQVT
                     GEPTRVASSFVKGYEHLPVVIPGRK"
     gene            complement(2724..4280)
                     /locus_tag="PLO65_05645"
     CDS             complement(2724..4280)
                     /locus_tag="PLO65_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRD27764.1"
                     /translation="MPSILEMIADDFGTVPDILRLNAAAHPDKVALIQDDRSMTWGEL
                     DAMMDRVAAGLQRDGVAHTQALSVCATTSLEYAALFLGGLRAGAAVAPIAPSSTPEQI
                     VAMVNDSGASLFFLDKGVAAEMGAALKDVSARLVALDGSDVGQAFYDWLPPAGARPAP
                     VRIDRKDPFNIIYSSGTTGTPKGIVQSHAMRWRHLAPREDGIGFGPDAVAFISTPLYS
                     NTTLVHVFPALAGGGTLVMLAKFDALKWLQAAQKHRVTHAMLVPVQYRRLLEHPDFDK
                     YDLSSFVMKFATSAPFPAELKKQAIERWPGGLIEYYGMTEGGGGFMLQAHLYPDKLHT
                     VGQVTPGAIIKMIDEEGAEVGPNEVGEIVGRSAATMSGYHNQPGKTAEAQWTDPKTGE
                     VYIRTGDVGRLDEDGFLTLMDRRKDMIISGGFNVYPSDLEQVILQHPAVAEAAVIAAP
                     SDAWGETPVAFVALKAGQAATADEIKTFTNGRVGKTQRLSDLVLIDSLPRSHIGKVLK
                     RELRDAYVAG"
     gene            complement(4400..6466)
                     /locus_tag="PLO65_05650"
     CDS             complement(4400..6466)
                     /locus_tag="PLO65_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD27765.1"
                     /translation="MAAINSVTELTRDGDIAIITLNSPPVNALSANVRDGLFEGFKAA
                     IADDAVKAIVLICAGRTFIAGADISEFGGASKGASLFDVQNMMENSPKAVIAAIHGTA
                     LGGGLEVALTAHYRVAVPSAKCGLPEVNLGLLPGAGGTQRLPRIVGAEKALEMMTSGA
                     HVPAKECLAMGLVDSLAEEGKLLEGAVAFARKVLDEKMPLKKVRDREVAGGSPEVFAA
                     FRKANARKFRGFNAPEANIKCVEASLLPFEEGLKVERQLFMELMTGAQSAAQRYVFFA
                     ERLANKIPDVPDDTAEIPVKKVGIIGAGTMGGGIAMNFLNVGIPVTIVETKQEALDRG
                     LGVIRKNYENTAKRGRLTMEQVEQRMGLLTGTLALEDLADSDLVIEAIFENMAVKKEV
                     FGKLDGIVKQGAILATNTSALNIDEIATAVKRPEAVIGLHFFSPANVMRLLEVVRADH
                     TSKEVIATSMKLAKKIGKVAVLVGVCPGFVGNRMLFQRQNQAQRLVMEGAMPWDVDRV
                     LYDFGFPMGPFAMSDLAGLDIGWNKEASKGETIRDQLCELDRRGQKTGAGYYDYDENR
                     NAIPSPVTEGIIKAFAAKQGLNQRDISDEEILERTIFPMINEGAKILEEGKAIRASDI
                     DIVWINGYGWPVYRGGPMHYADLVGLPKVVERLKHYEAQMGDFFKPSALLEKLAAEGK
                     GFKDLK"
     gene            6565..>7729
                     /locus_tag="PLO65_05655"
     CDS             6565..>7729
                     /locus_tag="PLO65_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27766.1"
                     /translation="MDLDFLPEHAEFRKEVRDWIEANYPAETRRKQEAGEHLGKEDIL
                     AWHRTLYKKGWLTPSWPVEYGGTGWDSVRKYIFSEELARAQTSQTSFGLGMIGPVLYT
                     FATPEQKAKFLPGTMNGDIWWCQGYSEPGAGSDLAGLSTRAERKTGEDGKDYYLVNGQ
                     KTWTTQGHFADWGFFLVRTDPTAKKQEGISFLLIDMKTPGITVRPIITIEGGHEVNDV
                     FLDNVKVPVENLIGEENKGWTVAKYLLGHERTGIAGVARSKRGIERLRELAAAELADD
                     AQPLIRDSDFRRKVAELEVDLAALEYTELRSLASESKGKGPGPEASLLKVKGTEVGQR
                     LTELTLEAAAHYGAPYARGMGDNIGPVGPDYAGKAAPTYFNMRKTSIFGGSNEI"
BASE COUNT         1257 a         2570 c         2635 g         1267 t
ORIGIN      
        1 aacagcacca tggccacggc gacgacgctg atgaacccga ccgccagcac catggccgtg
       61 acccccgcgc gccgggcgcc ggccaggtcg ccccggccag ccgccagccc cacccgcacg
      121 gtcgaggcca tgccgatgcc cagcggcacc atgaaggtga tcgaggcgaa gttcagcgcg
      181 atctggtgcg ccgccacggc gatggcgccg aaggtcccca tgaccagggt catggtgttg
      241 aacagcatgg cctcgaagat catcgtcatg ctgatcggca tgcccagccg gatcagctcc
      301 cgcacgcgga ccgggacggg ccgatgggcg cggcggaaga tgcggtaggc ccgcaggctc
      361 ggggtcagcc agatcaccac caccatggcg gcgaactcga agatgctcga gatcgccgtg
      421 gccatgcccg aaccgagcag gcccagcttc ggcaggccga agtggccgaa gatcaggcca
      481 tagccgagga agccgttgac cccgatcgcg gccagcatga cccacagggc cgccgtgggc
      541 ttgcccaggg cggcggtgaa gctgcgcagc gcctggtagc cgagggcgaa cggcaggccg
      601 atggccagca tcgagacgaa caccccggcc ccgcgcgcca gctccgggtc ctggcccagg
      661 tgcgagagga tccagcgcgc gttgagcatg accagcatca gcggcagggc ggccagcatg
      721 gtcacccaca gacccatgcg cacggtggcc cgcacgcgcg cccggtcgcc cggccaggcg
      781 ccgaggatct gggccacgac cggagagacg gccatgcccg gcccgctgcc gatcagccag
      841 gcgaaataga acacggtgtt gccgatcgcc gcggcggcca gggcctcgcg gctgagtcga
      901 cccagcagca ccacgtcggt ggtcatgatg gccatctggg ccagctgcga gccggccatc
      961 ggcgcggcca gcaccatcag cgcccgggcc tcgatgagcc agggcgttcg tcgtgggaca
     1021 gggcggtcga aaatcatgtc ggtcacggtc ggagactttc agataagccc gaacccgaac
     1081 gatgctggat ggcggaaacg aaaaatccct ccagagcggt cgggctcggg agggacgggc
     1141 gctggatcga aggtgcgatc taggcggcgt tttcctcgcg agcagacgcg gtgcaaccca
     1201 cgcccttggg cgtggagaag gccggtcgaa gggtcatgca ttgcgacgtc tgcatggatc
     1261 ctgctcgcga ttcgacgggc cccatgcccg tccggtggcc actcgttacc cgtgtaacgc
     1321 cgtcccgtca aatggcggcg ggagggcccc gatcatttgc ggccggggat gaccaccggc
     1381 agatgctcgt agcccttgac gaaggacgag gccacgcggg tcggctcacc cgtcacctgg
     1441 atgtcgggga agcgggccag gatttcctcc cagatgatcc gcagctgcag ttcggccagg
     1501 cggttgccga cgcagcggtg gatgccgaag ccgaacgaca ggtgctggcg cggccgctcc
     1561 cggtcgatga tgtagctgtt ggggttttcg atgaccgcgt catcgcggtt gcccgagaca
     1621 taccacatca ggaccttgtc gcccttgcgg atcttctggc cctgcagctc gatgtcctgc
     1681 agcgcccgcc ggcgcatgta ggccagcggc gtctgccagc ggatggtctc gctgaccatc
     1741 gacgggatca gcgacgggtc ggccttcagc ttggcgaact ggtcggggtt ctggttgagg
     1801 gccaggatgc tgccggtgat ggtgttgcgt gtcgtgtcgt tgccgccgac gatcagcagg
     1861 atcaggttcc cgaggtactc catgcggtcc atgttgcgcg tggattcgcc atggcagagc
     1921 atcgagatca gatcgttctt cggcggggcg ttgacccgct cgttccacag ccgggtgaag
     1981 tattcgacgc attcgaacag ggcggcccgg cggtgatcct cgcccttgta aaaggggctg
     2041 aacttgctgg cggtggcggt gtcggaccag taggtcagct tgcgccggtc ttcccacggg
     2101 aagtcgaaca gcgtggccag ggtcatggtc gtcagctcga tcgagacctt gtcgacccag
     2161 tcgatctcct cgccgatcgg caggtcgtcg aggatcttcg ccgcgcgttc gcggatgatc
     2221 ggctctaggt actgcaggtt gcccggcgag acgacggggc tgaccgtctt gcgctgcacg
     2281 tcgtgcttgg gcgggtccat ggcgatgaac atcggcaggc ggaagtcctc agacccctcg
     2341 gccagggtga tgccgccctc ggagctgaac acctggtggt tggtgtcgac cgccatgatg
     2401 tcgttgtacc gggtcaccga ccagtagggg ccgaactcgt gctccgcctg gtagtggacg
     2461 ggcgcctcct tccgcaggcg ctcgaagtag ggccacatgg tgtcggtctg gaacagcagc
     2521 gggtcggcga ccttgatctc cgacagcggc atgctccagg cgtgctcggc ggcggtctgc
     2581 ttcagatcca cggacccgtc gctcatgatc gttctccccg ttcgttttcg ccactttgac
     2641 ccttcccgcg cgtcagggcc agaggcgggg gtgcgcaaaa cggggagcga ggccgaccgc
     2701 cggcgggaac cgtcagccga ctcctatccc gccacgtagg cgtcgcgcag ctcgcgcttg
     2761 agcaccttgc cgatgtggct gcgcggcagg ctgtcgatca gcaccaggtc ggacaggcgc
     2821 tgggtcttgc cgaccctgcc gttggtgaag gtcttgatct cgtcggcggt cgccgcctgg
     2881 ccggccttga gggccacgaa ggccacgggg gtctcgcccc aggcgtccga cggcgcggcg
     2941 atcacggcgg cctcggccac cgccgggtgc tgcaagatca cctgctccag atcgctcgga
     3001 tagacgttga agccgcccga gatgatcatg tccttgcgac ggtccatcag ggtcaggaag
     3061 ccgtcctcgt cgagccggcc gacgtcgccg gtgcggatgt agacctcacc ggtcttcgga
     3121 tcggtccact gggcctcggc ggtcttgcca ggctggttat ggtagccgct catggtcgcc
     3181 gccgagcggc cgacgatctc gccgacctcg ttggggccga cctcggcgcc ctcctcgtcg
     3241 atcatcttga tgatcgcgcc gggcgtcacc tggccgacgg tgtgcagctt gtcgggatag
     3301 aggtgggcct gcagcatgaa gccgccgccg ccctcggtca tgccgtagta ttcgatcagc
     3361 ccgcccggcc agcgctcgat ggcctgcttc ttcagctcgg ccgggaacgg ggcgctggtg
     3421 gcgaacttca tcacgaagct ggacaggtcg tacttgtcga aatcggggtg ctcgagcagc
     3481 cggcggtact gcaccggcac cagcatggcg tgggtcacgc gatgcttctg ggcggcctgc
     3541 agccatttca gggcgtcgaa cttggccagc atcaccagcg tcccgccgcc ggccagggcc
     3601 gggaagacgt gcaccagggt ggtgttggag tagagcggcg tggagatgaa cgccaccgcg
     3661 tccggtccga agccgatgcc atcctcgcgg ggcgcgaggt gccgccagcg catggcatgg
     3721 ctctgcacga tgcccttggg cgtgccggtc gtgcccgacg aatagatgat gttgaaggga
     3781 tccttgcggt cgatccgcac cggcgcgggc ctcgcccccg ccggcggcaa ccagtcgtag
     3841 aaggcctgcc ccacgtcgga gccatccagc gcgaccagcc gggccgacac gtccttgagc
     3901 gccgcgccca tttcggcggc cacgcccttg tcgaggaaga acaggctggc gccggagtcg
     3961 ttgaccatcg ccacgatctg ttcgggcgtc gacgacggcg cgatcggcgc cacggccgct
     4021 ccggcgcgca gtccgccgag aaacagcgcc gcgtactcga gcgaggtcgt ggcgcagacc
     4081 gacagcgcct gggtatgcgc caccccgtcc cgctgcagcc ctgccgccac gcggtccatc
     4141 atcgcgtcca gctcgcccca ggtcatcgac cggtcgtcct ggatcagggc gaccttgtcc
     4201 ggatgcgcgg cggcgttcag ccgaaggatg tccggaaccg tgccgaagtc gtcggcgatc
     4261 atctcgagga tggagggcat ggggtagctc gatctggcgg cgagggggag ccaagggagt
     4321 aggtcgcagg gcgtagggag tagcaaatcg aggcgcggag acgccggtcg actactccct
     4381 actccctaac ggctacgccc tacttcaggt ccttgaaccc cttgccttcg gcggccagct
     4441 tctcgagcag agccgagggc ttgaagaagt cgcccatctg ggcctcgtag tgcttcagcc
     4501 gctcgaccac cttgggcagg ccgaccaggt cggcgtagtg catcggaccg ccgcggtaga
     4561 ccggccagcc gtagccgttg atccagacga tatcgatgtc cgaggcccgg atcgccttgc
     4621 cctcctcgag gatcttggcc ccctcgttga tcatcgggaa gatggtccgc tcgaggattt
     4681 cctcgtcgct gatgtcccgc tggttcaggc cctgcttggc ggcgaaggcc ttgatgatgc
     4741 cctcggtgac gggcgagggg atcgcgttgc ggttctcgtc gtagtcgtag tagccggcgc
     4801 cggtcttctg gccgcggcgg tcgagctcgc agagctggtc gcggatcgtc tcgcccttcg
     4861 aggcttcctt gttccagccg atgtccaggc cggccaggtc gctcatcgcg aacggcccca
     4921 tcgggaagcc gaagtcgtac agcacccggt cgacgtccca cggcatggcg ccttccatga
     4981 ccagacgctg ggcctggttc tggcgctgga acagcatgcg gttgccgacg aagccggggc
     5041 agaccccgac cagcacggcg accttgccga tcttcttggc cagcttcatc gaggtggcga
     5101 tgacctcctt ggaggtgtgg tcggcgcgga ccacttccag caggcgcatg acgttggccg
     5161 gcgagaagaa gtgcaggccg atgacggcct ccggacgctt caccgccgtg gcgatctcgt
     5221 cgatgttcag ggccgaggtg ttggtggcca ggatcgcgcc ctgcttgacg atgccgtcca
     5281 gcttgccgaa cacctccttc ttgaccgcca tgttctcgaa gatggcctcg atcaccaggt
     5341 cgctgtcggc caggtcctcg agcgccagcg tgccggtcag caggcccatg cgctgctcga
     5401 cctgttccat ggtcaggcga ccgcgcttgg cggtgttctc gtagttcttg cggatcacgc
     5461 ccaggccgcg gtcgagcgct tcctgcttgg tctcgacgat ggtcaccggg atgccgacgt
     5521 tgaggaagtt catggcgatg ccgccgccca tggtgccggc gccgatgatg ccgaccttct
     5581 tgaccgggat ctcggccgta tcgtcgggca cgtcgggaat cttgttggcc agccgctcgg
     5641 cgaagaacac gtagcgctgg gcggccgact gggccccggt catcagctcc atgaacagct
     5701 ggcgttcgac cttcagacct tcctcgaagg gcagcagcga ggcctcgacg cacttgatgt
     5761 tggcttccgg cgcgttgaag ccgcggaact tgcgggcgtt ggccttgcgg aaggcggcga
     5821 acacctccgg cgagccgccg gccacctcgc ggtcgcggac cttcttcagc ggcatcttct
     5881 cgtcgagcac cttgcgcgcg aaggccacgg cgccctcaag cagcttgccc tcctcggcca
     5941 ggctgtcgac caggcccatg gccaggcatt ccttggccgg cacgtgcgcg ccgctggtca
     6001 tcatctccag cgccttctcg gcgccgacga tgcgcggcag ccgctgggtg ccgccggcgc
     6061 ccggcagcag tccgaggttc acttccggca ggccgcactt ggccgaggga acggccacgc
     6121 gatagtgggc cgtcagggcc acttccaggc cgccgccgag cgccgtgccg tggatcgcgg
     6181 cgatcacggc cttggggctg ttttccatca tgttctggac gtcgaacagg ctggcgccct
     6241 tcgaggcccc gccgaactcg ctgatgtcgg cgccggcgat gaaggtgcgg cccgcgcaga
     6301 tcagcacgat ggccttgacc gcgtcgtcgg cgatggcggc cttgaagccc tcgaagagcc
     6361 cgtcgcggac attcgccgac agggcgttca ccggcggcga gttcagcgtg atgatggcga
     6421 tgtcgccatc gcgggtcagt tcggtgacgg aatttattgc ggccatgacg cctcacccct
     6481 atcgttcaat ttgaaacgct tgtttgagcg caaactgccg agccgggccg cgagagtcca
     6541 gcgcgcaaca cagggagaga caccgtggat ctcgatttcc ttcccgagca cgccgaattc
     6601 cgcaaggaag tgcgcgactg gatcgaagcc aactatccgg cggagacccg ccgcaagcag
     6661 gaggccggcg agcatcttgg caaggaggac atcctcgcct ggcaccgcac gctgtacaag
     6721 aagggctggc tgacgccgtc ctggccggtc gaatacggcg gcacgggctg ggattcggtc
     6781 cgcaagtaca tcttcagcga agagctggcc cgggcgcaga ccagccagac cagcttcggc
     6841 ctcgggatga tcggaccggt gctctacacc ttcgccacgc ccgagcagaa ggcgaagttc
     6901 ctgcccggca cgatgaacgg cgacatctgg tggtgccagg gctactccga gcccggcgcc
     6961 ggctcggacc tcgcgggcct gtcgacccgc gccgagcgca agaccggcga ggacggcaag
     7021 gactactacc tggtcaacgg ccagaagacc tggaccaccc agggccactt cgccgactgg
     7081 ggcttcttcc tcgtccgcac cgatccgacg gccaagaagc aggagggcat ctccttcctg
     7141 ctgatcgaca tgaagacccc cggcatcacc gtgcgcccga tcatcaccat cgagggcggc
     7201 cacgaggtca acgacgtctt cctcgacaat gtgaaggtgc ccgtcgagaa cctgatcggc
     7261 gaggagaaca agggctggac ggtggccaag tacctgctcg gccatgaacg caccggcatc
     7321 gccggcgtcg cccgctcaaa gcgcggcatc gagcgactgc gggagctggc cgccgccgaa
     7381 ctggccgacg acgcccagcc gctgatccgc gacagcgact tccgtcgcaa ggtggccgag
     7441 ctggaggtcg acctggccgc gctcgaatat accgagctga gaagcctggc ctcggagagc
     7501 aagggcaagg ggccaggccc ggaagcctcc ctgctgaagg tcaagggcac cgaggtcggc
     7561 cagcggctga ccgagttgac gctggaagcg gccgcccact acggcgcgcc ctacgcccgc
     7621 ggcatgggcg acaacatcgg cccggtcggc cccgactacg ccggcaaggc cgccccgacc
     7681 tacttcaaca tgcgcaagac ctcgatcttc ggcggctcca acgagatcc
//
LOCUS       DAORNX010000091         7724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_6993_length_7724_cov_7.135481, whole genome shotgun sequence.
ACCESSION   DAORNX010000091 DAORNX010000000
VERSION     DAORNX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6993_length_7724_cov_7.135481"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..667)
                     /locus_tag="PLO65_05660"
     CDS             complement(38..667)
                     /locus_tag="PLO65_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD27767.1"
                     /translation="MKLYDSLRAPNPRRVRWFLAEKGAEDQVEIIDVSIFKGEHRQPD
                     YIGKAGLANVPMLEMDDGTAITESVAICRYLESVFPEPNMFGRDPKETAIIEMWCRRT
                     EMMLATPLMMTVRHSHPALAALEKQIPEIAETNRAGATRTIRFFDRRLAESEFIAGDR
                     ITMADILAATAIDFSRMAKFAIPEEMANATRWYAAMMARPAALAGTVAA"
     gene            complement(691..2019)
                     /locus_tag="PLO65_05665"
     CDS             complement(691..2019)
                     /locus_tag="PLO65_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRD27768.1"
                     /translation="MFQPANAQSLIGVVFVLGLCWALSEDRKRFPLKLTIASLAIQVG
                     LVLVLFGLPRLDAAHNVYNPLYWLGAGAQGVLHAAGSAVEGLGTATRAGTSFVFGFLA
                     GGDQPFAVTGPGGVFIFAFQVMPVILVVCALSALLWHWKILKWITQGFGLMFQKTLGL
                     RGPIGLATAATIFMGQVEGPIFIRAYLDRLSRSELFMLISVGMACVSGSTMVAYATIL
                     KDVLPDAAAHVLTASIISAPAGVLLARILVPSDPTEADDSLLDADDKRYDSSVDALIK
                     GTNDGLTIVMNVGATLIVFVALIAMADLILAALPTLWGAPMSIERGLGAVFAPVAYAL
                     GLPWSEAPTGGLLLGAKLVLTEFTAFIRMSELPPEALSERSRMIMTYALCGFANIASV
                     GINVAGYGVLVPTRRHEVMAMVWKAMLAGFLATCLTASLVGLMPAALFGG"
     gene            complement(2116..4179)
                     /locus_tag="PLO65_05670"
     CDS             complement(2116..4179)
                     /locus_tag="PLO65_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRD27769.1"
                     /translation="MKPVFAALAVLVGLAGAAPALADEGMWTFDAFPAARVKAAYGVD
                     IDQRWLDRVQAAAVRIPGCSASVVSPDGLVLTNNHCVVDCLVALSDERNDFMKDGFLT
                     AARAEERTCPGMYGEILVSITDVTDRIQAATAGKSGADYGRARDAATIAAEAGVCQGA
                     TGSRCQTVSLYRGGRFAVYRYRRYNDLRLVFAPEFATAFFGGDPDNFNFPRYDMDAAF
                     LRLYDGGKPVRTPAHLTWNPAPPKAGEVTFVAGNPGSTERLLTMSQLETARDLAVPIG
                     QLQRAELRGRLIEFGRRGHEARRMVADPVFSIENSFKVSFGRQLALTDRAFMDGKRKA
                     EAELRARVAADPALAAEIGDPWAELEAVQAAYARDFLPYRQLEAEAGRGARLFDWART
                     LVRAAEERSKPSADRLPEFSETRLPIVERRLLAATKVEPELERLYMEFWLLKLREYLG
                     TDAATLDLMLGKESPEGLAGRLVSGSRLGDPAVRKALWDGGLEAVKASDDPMIQYALK
                     VDAAARAARKTWEADVTAPTNRAAERVARARFAVYGDSVYPDATFTLRLSYGKVAGWS
                     WRGRETGPVTTFGGLYARATGAEPYALAPRWAAAKDRLNPDTVFDFVTTNDIIGGNSG
                     SPVINARGEVIGAAFDGNIHSIAGAYGYDGTTNRTVVVSTAAITEALREVYGREALVK
                     ELLRK"
     gene            4356..5525
                     /locus_tag="PLO65_05675"
     CDS             4356..5525
                     /locus_tag="PLO65_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD27770.1"
                     /translation="MSLALATLIYEWRRYMAAVVALAFSGLLVLAQVGMFMGIGKAFT
                     AQIDRASADVMVLGPGAKALFNTGGASGLPRRLIPLVYSHPEVLQVAPLEGSGGRFMN
                     IVPTDPLNPGKTPANRKVEFVDVNIIDAIPGYVTVPTDYTNEMIEALRQPYAVAVDQT
                     SLKRLGVKKGDKALYNGKTIFIAVVTTDYPNIMQATLVMSRDTLRLLGEANTGPRSGP
                     LMVKIADPSRAEIVAAQLNKLGKGQFRAWTREELAEANSGVLFQEQFIGIMLGFSAFL
                     GIMIGVAITWQTLRGAIMANIKEFASLRALGVSMGSLRRIVIELSFWVGVAGIFASGL
                     LTWGVSLLAQSGGLPMAFPPSLIAIVSMFLLSIAIISGFLSLGVLKQSQPADLLR"
     gene            5534..6283
                     /locus_tag="PLO65_05680"
     CDS             5534..6283
                     /locus_tag="PLO65_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD27771.1"
                     /translation="MINNNGHKLGEKAIEARGLVKRFKTGRTFIEVLKSVDFDARHGD
                     VTMVMGPSGSGKSTLIAALSGLLRPEEGKVRALEADDLWKWSSGRIDRFRLDHCGFIF
                     QGFNLFPALTARQQVMIALHYQGASQAEARARAQAALEEVGMGPKVNQRPSELSGGEK
                     QRVAIARALAKNPNLIFADEPTSALDGENGQVVIRLLRRAATERGAAVICVTHDPRLE
                     AYADRVIHLEDGRILDDVRRTPDANPAPLSH"
     gene            6301..7296
                     /locus_tag="PLO65_05685"
     CDS             6301..7296
                     /locus_tag="PLO65_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD27772.1"
                     /translation="MFGFLRSRWFWVLTIVAAAVVGGGLFMKSQAEAKKKKIEAAASQ
                     TAPSPYAAIANGKTDVEGGIIQVAARRGGVVKAVYVQEGDRVVAGQVLARQEDDDPRL
                     AVARASAQVAQAQAQIGVMQVKLDAARREYARLEGLSDSNFVARQRLDQQNDQIRDWQ
                     AQIVAQRAAVATAVAALREAEYALELTVIRAPTNGRIVRRYANPGAGASTLNVSNMFD
                     LEPDTARIVRAEIVEADIPNVTDGQPVEIQPESDPGKVYVGKVLRRAAVFGARKLASD
                     DPTQRSDERVVEIVVSADGAPLLIGQRVLVKFMKPGFKAGEKRAAPKSDPAAKAG"
     gene            complement(7299..>7724)
                     /locus_tag="PLO65_05690"
     CDS             complement(7299..>7724)
                     /locus_tag="PLO65_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRD27773.1"
                     /translation="VEAQVLPERKAAVVERLRGEGFVVAMAGDGVNDAPALAAADVGL
                     AMGTGTDVAIESAGVTLLGGDLTGIARARRLSRATMRNIRQNLAFAFLYNAAGVPVAA
                     GVLYPVFGLVLSPQLAAAAMALSSVSVIANALRLRTVRL"
BASE COUNT         1236 a         2723 c         2609 g         1156 t
ORIGIN      
        1 gccaggacca gcgccgcggc cagcagcagc gcgcgcatca tgccgcgacc gtgccggcca
       61 gggccgcggg ccgggccatc atcgccgcgt accagcgcgt ggcgttggcc atctcctcgg
      121 ggatcgcgaa cttggccatg cgcgagaagt cgatcgccgt ggcggccaga atgtcggcca
      181 tggtgatccg gtcgccggcg atgaattcgc tctccgccag ccgtcggtcg aagaacctga
      241 tcgtgcgcgt cgcgccggcc cggttggtct cggcgatctc cgggatctgc ttttccagcg
      301 cggccagggc cggatggctg tggcgcacgg tcatcatcag cggcgtggcc agcatcatct
      361 cggtgcggcg gcaccacatc tcgatgatcg ccgtctcctt cggatcgcgg ccgaacatgt
      421 tgggctcggg aaacacgctt tccaggtatc ggcagatggc caccgactcg gtgatcgccg
      481 tgccgtcgtc catctccagc atcggcacgt tggcgaggcc cgccttgccg atatagtccg
      541 gctgcctgtg ctcgcccttg aagatgctca cgtcgatgat ctcgacctgg tcctcggcgc
      601 ccttctcggc caggaaccag cgcacccggc gagggttcgg cgcccgcagg ctgtcataga
      661 gtttcacctg tttcaccatc cttccgaagt tcatccgccg aacaacgccg ccggcatcag
      721 cccgaccagc gacgccgtca ggcaggtggc caggaagccg gccagcatgg ccttccacac
      781 catggccatc acttcatgcc gccgcgtcgg cacgagcacg ccgtaaccgg cgacgttgat
      841 tccgaccgaa gcgatattgg cgaaaccgca cagggcgtag gtcatgatca tccggctgcg
      901 ctcgctgagc gcctcgggcg gcagttcgct catcctgatg aaggccgtga actcggtcag
      961 caccagcttg gcgcccagca gcaggccgcc cgtcggcgcc tcgctccacg gcaggcccag
     1021 cgcataggcc accggcgcga acacagcgcc cagtccccgc tcgatgctca tcggcgcgcc
     1081 ccacaaggtc ggcagggcgg cgaggatcag gtcggccatg gcgatcaggg ccacgaacac
     1141 gatcagggtc gcgccgacgt tcatcacgat ggtcaggccg tcgttggtgc ccttgatcaa
     1201 ggcgtcgacc gagctgtcgt agcgcttgtc gtcggcgtcc agcaggctgt cgtcggcttc
     1261 ggtcgggtcg gacgggacga gaatgcgcgc cagcagcacg ccggccgggg cggagatgat
     1321 cgaggcggtc agcacatggg ccgcggcgtc gggcagcacg tccttcagga tcgtcgcata
     1381 ggccaccatg gtcgagccgc tgacgcaggc catgccgacc gagatcagca tgaacagctc
     1441 ggaccgcgac agccggtcga gataggcgcg gatgaagatc ggcccctcga cctggcccat
     1501 gaagatggtc gccgccgtcg ccaggccgat cggtccgcgc agcccgagcg tcttctggaa
     1561 catcaggccg aagccctggg tgatccattt gaggatcttc cagtgccaca gcagcgccga
     1621 cagggcgcag acgacgagga tcaccggcat gacctggaag gcgaagatga ataccccgcc
     1681 ggggccggtg accgcgaacg gctggtcgcc gccggcgagg aaaccgaaca cgaagctcgt
     1741 gcccgcgcgg gtcgccgttc ccagcccctc gaccgcgctg ccggccgcgt gcagcacccc
     1801 ctgggcgccg gcgcccagcc agtagagcgg attgtagaca ttgtgcgccg cgtccagtcg
     1861 cggcaggccg aacagcacga gcaccagccc cacctggatc gccagcgacg caatggtcag
     1921 cttcagggga aaccgcttgc gatcctcgga cagcgcccag cagaggccga gcacgaaaac
     1981 aacgccgatc aggctctggg cgttggcggg ctggaacatg aactccccct cggccgcgag
     2041 tcccctgccc gcggccaacg gtcgattgac tacgaaggcg ggcggccgcg caaggcgcct
     2101 gcttccgggc tgcccctact tccgcagcag ttccttcacc agggcctcgc gaccgtagac
     2161 ctcgcgcagc gcctcggtga tggcggcggt cgagacgacc accgtacggt tggtggtccc
     2221 gtcatagccg taggcgccgg cgatgctgtg gatgttgccg tcgaaggccg cgccgatcac
     2281 ctcgccccgg gcgttgatca ccggcgatcc ggagttgccg ccaatgatgt cgttggtggt
     2341 cacgaagtcg aacaccgtgt cagggttcag ccggtccttc gccgccgccc agcggggcgc
     2401 cagggcgtag ggctcggcgc cggtggcccg ggcgtagagc ccgccgaagg tggtcaccgg
     2461 cccggtctcg cgcccacgcc agctccagcc ggcgaccttg ccgtaggaca gccgcagggt
     2521 gaaggtcgcg tccgggtaga cactgtcgcc atagaccgcg aaccgcgccc gggcgacgcg
     2581 ctcggcggcg cggttggtcg gggcggtgac atcggcctcc caggtcttgc gggcggcccg
     2641 ggccgcggcg tcgaccttga gggcgtactg gatcatcgga tcgtccgacg ccttcacggc
     2701 ctccagaccg ccgtcccaaa gcgccttgcg caccgccgga tcgcccagcc ggctgccgct
     2761 caccagccgt ccagcaaggc cctcgggact ctccttgccc agcatcaggt cgagcgtcgc
     2821 ggcgtcggtt ccgagatatt cgcgcagctt gagcagccag aactccatat acagtcgctc
     2881 gagctccggc tcgactttcg tggccgccag cagtcgccgc tcgacgatcg gcaagcgtgt
     2941 ctcggaaaac tccggcagcc ggtcagcgga cggtttcgag cgttcctcgg cggctcgcac
     3001 gagggtgcgc gcccagtcga acagccgggc gccgcgtccg gcctcggcct ccagctggcg
     3061 gtacggcagg aagtcgcgcg cgtaggccgc ctgcaccgcc tcgagttcgg cccagggatc
     3121 gccgatctcc gccgccagcg ccggatcggc cgcgacccgc gcgcgcagct cggcctcggc
     3181 cttgcgcttg ccgtccatga aggcccggtc ggtcagggcc agctggcggc caaaggaaac
     3241 cttgaagctg ttctcgatgc tgaacacggg gtcggcgacc atccgccggg cctcgtgtcc
     3301 gcgtcgcccg aactcgatca ggcgtccgcg cagttccgct cgctgcagct ggccgatcgg
     3361 cacggcgaga tcgcgcgccg tctccagctg gctcattgtc aggagccgtt cggtggaccc
     3421 ggggttgcca gcgacgaagg tcacctcgcc tgccttcggc ggagccgggt tccaggtcag
     3481 gtgcgccggc gtcctcaccg gtttgccgcc gtcgtagagc cgcaggaagg cggcatccat
     3541 gtcgtagcga gggaagttga aattgtcggg atcgccgccg aagaaagcgg tcgcgaattc
     3601 cggcgcgaaa accagccgca gatcgttgta gcggcgatag cggtacacgg cgaaacggcc
     3661 gccgcggtac aggctgaccg tctggcaccg gctgccggtc gcgccctggc agaccccggc
     3721 ctcggccgcg atggtcgcgg cgtcgcgcgc cctgccatag tcggcgcccg acttgccggc
     3781 ggtggcggcc tggatgcggt cggtgacgtc agtgatcgag accaggatct cgccgtacat
     3841 gcccgggcag gtccgctcct ccgcgcgcgc ggcggtcagg aagccgtcct tcatgaagtc
     3901 attgcgctcg tcagacaggg cgacgaggca atcgaccacg caatggttgt tggtcaggac
     3961 cagcccgtcc ggcgagacca ccgacgcgga acagccggga atgcgcacgg cggcggcctg
     4021 gacgcggtcg agccaccgct ggtcgatgtc gaccccgtaa gcggccttga cccttgcggc
     4081 cggaaaggcg tcgaaggtcc acatgccctc gtcggcgagg gcgggagcgg cgcccgccag
     4141 tccgacgagg acggccaggg cggcaaaaac aggcttcatc gaaatcacgc gaaaaccaga
     4201 acaaaattga ccaacggcgg cggccataac gcgccgtgtc gcgcctggcc agcctttggc
     4261 gcaaggcctt accccgattt aacttggccg cgggggaact ccgttacaga accacagcgc
     4321 gttgaaatac gagcgctcgg ggagttgggt tcgtcatgtc gctggccttg gccacgctga
     4381 tctatgagtg gcgccggtac atggcggccg tggtggcgct ggccttctcg ggcctgctgg
     4441 tgctggccca ggtcggcatg ttcatgggca tcggcaaggc tttcacggcc cagatcgacc
     4501 gcgccagcgc cgacgtcatg gtgctgggtc cgggagccaa ggcgctgttc aacaccggcg
     4561 gcgcctcggg cctgccgcgc cgcctgatcc cgctggtcta cagccacccc gaggtgctgc
     4621 aggtggcgcc gctcgagggc agcggcggcc ggttcatgaa catcgtgccg accgatccgc
     4681 tcaatccggg caagacgccg gccaaccgca aggtcgagtt cgtcgacgtc aacatcatcg
     4741 acgccatccc cggctatgtg acggtcccca ccgactacac caacgagatg atcgaggcgc
     4801 tgcgccagcc ctacgcggtg gccgtcgacc agacctcgct caagcggctt ggcgtgaaga
     4861 agggcgacaa ggccctctac aacggcaaga ccatcttcat cgcggtggtg accaccgact
     4921 accccaacat catgcaggcg accctcgtga tgtcgcgcga caccctgcgc ctgctgggcg
     4981 aagccaatac cggcccgcga tccggaccgt tgatggtcaa gatcgccgac ccgtcccgcg
     5041 ccgagatcgt cgccgcccag ctcaacaagc tcggcaaggg ccagttccgc gcctggaccc
     5101 gcgaggagct ggccgaggcc aactccggcg tgctgttcca ggagcagttc atcggcatca
     5161 tgctcggctt ctcggccttc ctcggcatca tgatcggcgt ggccatcacc tggcagaccc
     5221 tgcgcggcgc gatcatggcc aatatcaagg agttcgcctc gctgcgggcg ctgggcgtca
     5281 gcatgggcag cctgcggcgg attgtcatcg aactgagctt ctgggttggc gtcgccggca
     5341 tcttcgcctc cggactgctg acctggggcg tctcgctgct ggcccagtcg ggcggcctgc
     5401 cgatggcttt cccgccgtcc ctgatcgcca ttgtgtcgat gttcctgctg tcgatcgcca
     5461 tcatctccgg cttcctgtcg ctcggcgttc tcaagcagag ccaacccgcg gacctgctgc
     5521 gatgagcccg tccatcatca acaacaacgg ccataagctc ggcgagaagg cgatcgaggc
     5581 ccgcgggctg gtcaagcggt tcaagaccgg ccggaccttc atcgaggtgc tcaagtcggt
     5641 ggatttcgac gcgcgccacg gcgacgtgac catggtcatg gggccgtcgg gctcgggcaa
     5701 gtcgacgctg atcgccgccc tgtcgggcct gctcaggccc gaggagggca aggtgcgggc
     5761 gctggaggcc gacgacctgt ggaagtggtc gtccggccgc atcgaccggt tccggctcga
     5821 ccactgcggc ttcatcttcc agggcttcaa cctgttcccc gccctgaccg cgcgccagca
     5881 ggtgatgatc gccctgcact accagggcgc ctcccaggcc gaggcccggg cccgcgccca
     5941 ggcggcgctc gaggaggtcg ggatgggtcc caaggtcaac cagcggccgt cggagctgtc
     6001 cggcggcgag aagcagcggg tggccatcgc ccgggccctg gccaagaacc ccaacctgat
     6061 cttcgccgac gagccgacct cggcgctcga cggcgagaac ggccaggtgg tcatccgcct
     6121 gctgcgccgg gccgccaccg agcgcggcgc ggcggtgatc tgcgtgaccc acgacccgcg
     6181 tcttgaggcg tacgccgacc gggtcatcca tctcgaagac ggccgcatcc tggacgacgt
     6241 tcggcgcacg ccggacgcca atccggcgcc gctcagccac tgactctgaa gggacgattc
     6301 atgttcggct ttctgcgtag ccgctggttc tgggtgttga cgatcgtggc cgcggccgtg
     6361 gtcggcggcg gcctgttcat gaagagccag gccgaggcca agaagaagaa gattgaggcg
     6421 gccgccagcc agactgcccc cagtccctac gcggccatcg ccaacggcaa gaccgatgtc
     6481 gagggcggca tcatccaggt cgccgcccgc cgcggcggcg tcgtcaaggc cgtctatgtg
     6541 caggaaggcg accgcgtcgt cgccggtcag gtcctggccc gccaggagga cgacgatccc
     6601 cgcctggccg tcgcccgcgc cagcgcccag gtggcccagg cccaggccca gatcggcgtc
     6661 atgcaggtca agctcgacgc cgcccgtcgc gaatacgcgc gtctcgaagg cctgtcggac
     6721 agcaacttcg tcgcccgcca gcggctcgac cagcagaatg accagatccg cgactggcag
     6781 gcccagatcg tcgcccagcg ggccgccgtg gccaccgccg tggccgccct gcgggaagcc
     6841 gagtatgctc tggaactgac cgtcatccgg gcgccgacca acggccgcat tgtccggcgc
     6901 tacgccaatc cgggcgccgg cgcctcgacc ctcaacgtct ccaacatgtt cgacctcgag
     6961 ccggacaccg cccgcattgt ccgcgccgag atcgtcgagg ccgacatccc caatgtcacc
     7021 gatggccagc cggtcgagat ccagccggag tcggatccgg gcaaggtcta tgtcggcaag
     7081 gtcctgcgcc gcgccgcggt gttcggggcg cgcaagctcg cctcggacga cccgacccag
     7141 cgcagcgacg agcgggtggt cgagatcgtc gtcagcgccg acggcgcccc cctgctgatc
     7201 ggccagcggg tgctggtcaa gttcatgaag cccggcttca aggcgggcga gaagcgcgcc
     7261 gcgccgaagt ccgacccggc ggccaaggcc gggtaggcct acagcctgac cgtccgcagc
     7321 cgcagggcgt tggcgatcac gctgaccgaa ctgagggcca tggccgccgc ggccagttgc
     7381 ggagacagca ccaggccgaa gaccgggtac agcacgccgg ccgcgaccgg cacgccggcg
     7441 gcgttgtaga ggaaggcgaa ggccaggttc tggcggatgt tgcgcatcgt cgcccgcgac
     7501 agccgccggg ctcgcgcgat accggtcagg tctcccccga gcagggtcac cccggcgctc
     7561 tcgatggcga cgtccgtgcc cgtgcccatg gccaggccaa cgtcggcggc cgccagggcc
     7621 ggggcgtcgt tgacgccgtc gccggccatg gcgaccacga agccttcgcc gcgcagccgc
     7681 tcgaccacgg ccgccttgcg ctcgggcagg acctgtgcct cgac
//
LOCUS       DAORNX010000092         7669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7052_length_7669_cov_8.972682, whole genome shotgun sequence.
ACCESSION   DAORNX010000092 DAORNX010000000
VERSION     DAORNX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7052_length_7669_cov_8.972682"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLO65_05695"
     CDS             complement(<1..493)
                     /locus_tag="PLO65_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD27774.1"
                     /translation="MNSDEYQSFDAVGLAALVARRDVSPSELLDTALERLDEINPAIN
                     AVIRVVEDQARTAIAEGLPQGPLTGVPFLIKDISQQMKGLPTCGGSRLFLDAAPAPAD
                     SALVAAYRRAGLVLFGKTNTPEFGAAATTEPVAFGATRNPWNLERTSGGSSGGAAAAV
                     AAGI"
     gene            complement(547..1407)
                     /locus_tag="PLO65_05700"
     CDS             complement(547..1407)
                     /locus_tag="PLO65_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD27775.1"
                     /translation="MTQTDIHPAGPVKSNGAAPLDQAAPLILVSREGGIAWLTLNRPD
                     AANSIDAPLAKAFRAAIAALDADESVRVLVLAGAGRMFCAGGDLTTFAGQGDGAARYV
                     EGLIHDLHAGLEQLASFHAPIVAVVHGAAAGAGLGLAMAADIVLADAGSRFVMAYTRA
                     GLTPDGGTSWMLPRLVGMRRALELTLTNRTLSAREALELGLVTEVTTETALAARARAV
                     ALELAAGPTAAYATARRLIRTALENDYAVQLGQEARSIVASFNTADGLEGVRAFRERR
                     TPVFPGAAKP"
     gene            complement(1404..2549)
                     /locus_tag="PLO65_05705"
     CDS             complement(1404..2549)
                     /locus_tag="PLO65_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27776.1"
                     /translation="MIARPAMAGALPFKPEHELFRASARRFLETECAPHRDAWEEAGM
                     APRSIWRRAGELGLLCFRAPEAYGGANADILFSVALMEEQVRAGVVGPMMSLHNDVVA
                     PYVERYGSEAQKARVLPRMASGEWIGAIAMTEPSGGSDLRGMTTRARRDGDDYIITGH
                     KTFISNGACADIVVLAAQTDGGISLFLIETPGLEGFTHGEPLEKLGQWSADTTELFFD
                     GARVPASCLLGEIEGRGFPQLMDRLVEERLMTAVGAVATMETAIAHTIAYTSDRKAFG
                     QRVIDFQNSRFRLAEAQTELVVCRVFLARCMRRFMAGDLSADEAAMLKLRTTDLQNRV
                     VDQCLQLHGGYGYMLEYPIARMWLDSRISRIYGGSNEIMKEIIGRTL"
     gene            complement(2564..3706)
                     /locus_tag="PLO65_05710"
     CDS             complement(2564..3706)
                     /locus_tag="PLO65_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27777.1"
                     /translation="MIESPYYTPEHEIFRESVRRFIERECLPNRKAWQKDLKVSRDVW
                     TRAGEAGLLCSKVAPEFGGPGGDLFHCMVIMEEQTRAGFNELAFYLHSDIIAPYIELY
                     GTEAQRRTWLPRMVTGEITSAIAMTEPGAGSDLQAIRTRAVREGDHYVLNGQKTFISN
                     AQLADIIVVAAKTDPEAGGRGVSLLILEPKDAKGFSLGRNLRKIGCKAQDTSELFFED
                     VRIPAENLLGGEEGRGFYQLMQRLPEERLIMAMSAVANMEFAVEEAVAYARQRMVFGK
                     PLIDHQDARFKLAECKTTVAFARTFLADCVDRFIEGTFDARDGAMAKWWTTERSCEVI
                     DDCLQVFGGYGYMKDFPISRRYADARLGRIAGGTNEIMKDLIGRSL"
     gene            complement(3789..6050)
                     /locus_tag="PLO65_05715"
     CDS             complement(3789..6050)
                     /locus_tag="PLO65_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD27778.1"
                     /translation="MNDKLSRQVARRAMLGIGVSAAALMAAPAFAQDAPVKTAPSNSG
                     DYTVESITITAQRRSEENIKVPVAVTALGAEELQDITANNVTDIAFKIPNVLMSSGSI
                     SPSISIRGVSSQSNYAAGFPPAVGVYVDEVYQGRDPTFNVSLNDIERIEVLRGPQGTL
                     YGKNTIGGAINLITGDPSNDFTAFGDVQFGNYDMSQFRATVGGAIIPDLLMVRLSVVH
                     AEREGYITNTFRNTELNDFSGNGARLVIGSQPNDRLKIRLSADYFKQVGSQAAETGPV
                     SFLVPLPIFATVPPQDPSDNVVQLNTDSFAKRELGGGSVRVDYDLDSVTLTSITAYRS
                     YMSDFEDDSDGIQLDGFNVGREENGENFSQELRLTSTGEGPFAWIGGFYFYAENTENN
                     RRIRTGPNFPQLLAGLTLPGYAERARTESRIEGTSWALFASGTYQLSEQLRLAGGLRY
                     TTENKDFTYHQYYTQTYGPGGPCGAGGLFIPCFAVNIPQRAESYNEGQLTGDISLSYD
                     YSDDQVFSIRYSRGFKAGGFNTDVISPPFNPADSLGFAPEFVDSYEAGYKSFWFDRRL
                     SLNVAAFYLSWKDKQEQIDLGTSFLVRNAASASNKGVEIELTARPTRYLTLDFNGAMQ
                     RAKYEEFPADPASEGKSFGLPEFTGSAGAQFIMPVNDAIEFYLRGDVIYRGESYVNTA
                     NTLANEATTRINGRIGFQSTTGGWGAYLWGKNIADEITPSGGTFRQVGIYGYTVRGAD
                     LGRTWGVELRKRF"
     gene            complement(6162..>7669)
                     /locus_tag="PLO65_05720"
     CDS             complement(6162..>7669)
                     /locus_tag="PLO65_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRD27779.1"
                     /translation="APAARPMMRTAAGVALGLASGGLATLAFPPFELWPLIFVAFVPM
                     VVAQHRILPKGLGPLAPGAAIGAYYAGQLSAGLSDGGVAWYIQLWPLYVGLLIAALCW
                     PGRHFHSRTAYRWLVLSFPVAWVGIDAARALSGVDVLAGTWGNPVYALYAQPWLLQPL
                     TVFGLFGLELLLLVINFAVAGLVIALLDRRRAPEGAPVLPMARALRAAGVVGALAVAW
                     TIMSLVQFQNHPATLRVGTVQANAPRDPEEELARNVAATRALAAQGAKLVVWREGMLK
                     FHPLRERTAELKALAAETGAHIALGYGLKQPDGLRLNEAVLFAPDGQVFGPYGKSHPG
                     RFAGDQSDTGGDYKVYDTALGKVATIICYDLDFTDTAREMARRGAGVIAVPSNDVPAI
                     ATTHYTHLVFRAIENRLPMAKADSLFDTAIVDPYGRLLARNVNPLSMAEARRVTATRE
                     LAAQTLIADVPLATGPTVTTRLGDWMGWLAIGAMLVAAGSQVVRRFRRRDA"
BASE COUNT         1203 a         2501 c         2699 g         1266 t
ORIGIN      
        1 cgatgcccgc cgcgaccgcc gcggccgcgc cgccggatga gccgccgctg gtccgctcca
       61 gattccaggg attgcgggtc gccccgaacg ccacgggttc ggtcgtcgca gcggcgccga
      121 actccggggt gttggtcttg ccgaacagga cgagaccggc ccgacggtag gccgcgacga
      181 gcgcgctgtc ggccggggct ggggcggcgt cgagaaacag gcgggaaccg ccgcaggtgg
      241 gcagtccctt catctgctgc gagatgtcct tgatcaggaa cgggactccg gtcagcgggc
      301 cctggggcag gccttcggcg atcgcggtcc gggcctggtc ctcgaccacc cggatcacgg
      361 cgttgatcgc gggattgatc tcgtccagcc gttcgagggc ggtgtcgagg agctccgagg
      421 gactgacatc gcggcgggcc accagcgccg cgaggccgac ggcgtcgaag gactggtact
      481 catcactgtt caacggtcac gctcctgggc gatgggcaag ggccggggcg gtcgaggcgg
      541 ccggtgtcag ggcttggcgg cgcccgggaa gacgggggtc cgccgctcgc ggaaggcccg
      601 cacgccctcc aggccgtcgg cggtgttgaa ggaggcgacg atcgaacgcg cttcctggcc
      661 gagctggacg gcgtagtcat tctccagggc ggtccggatc agtcgccgcg cggtggcgta
      721 ggcggccgtc ggaccggcgg ccagctccag cgccacggcg cgggcccggg ccgcgagcgc
      781 cgtttctgtc gtcacctccg tgaccaggcc cagctcgagg gcttccctgg cgctgagggt
      841 ccggttggtc agcgtcaact cgagcgcccg gcgcatgccg accagcctcg gcagcatcca
      901 gctggtgccc ccgtccggcg tcagcccggc ccgggtatag gccatgacga accggctgcc
      961 ggcgtcggcc agcacgatgt cggcggccat ggccaggccg aggcccgcgc ccgccgccgc
     1021 gccgtggacc acggccacga tcggcgcatg gaaagacgcc agctgctcca gccccgcatg
     1081 caggtcatgg atcagcccct cgacatacct ggccgcgcca tcgccctgac cggcgaatgt
     1141 ggtcaggtcg ccgccggcgc agaacatccg gcctgcgcca gccagcacca gcacccgcac
     1201 gctttcgtcc gcatcaaggg cggcgatggc cgccctgaaa gccttcgcca ggggggcgtc
     1261 gatcgagttg gcggcgtcgg gccgattcag ggtgagccag gcgatcccgc cttcgcggct
     1321 caccaggatc agcggcgccg cctggtccag cggcgccgcg ccgttggact tcaccgggcc
     1381 ggccgggtga atgtcggtct gggtcataga gttctaccga tgatttcttt catgatctcg
     1441 ttgctgccgc cgtagatcct ggaaatccgg ctatcgagcc acatccgggc gatcggatac
     1501 tcgagcatgt atccatagcc gccatgcagc tggaggcatt ggtcgacgac gcggttctgc
     1561 aggtcggtcg tgcgcagctt gagcatcgcg gcctcgtcgg ccgacaggtc tccggccatg
     1621 aacctgcgca tgcagcgggc caggaacacc cggcagacaa ccagctcggt ctgggcctcg
     1681 gccagccgga agcgactgtt ctggaagtcg ataacccgct gtccgaaggc cttgcggtcg
     1741 ctggtgtagg cgatggtgtg ggcaatggcg gtttccatcg tggccaccgc gccgacggcg
     1801 gtcatcagcc gctcctcgac cagccggtcc attagctgcg ggaagccgcg gccctcgatc
     1861 tcgccgagca ggcaggaggc cgggacccgc gcgccatcga agaacagctc ggtcgtgtcg
     1921 gccgaccact gacccagctt ctcgagcggc tcgccgtggg tgaacccttc gaggcccggt
     1981 gtttcgatca ggaacagact gatgccgcca tcggtctgcg ccgccagcac gacgatgtcg
     2041 gcgcaggccc cgttggagat aaaggtcttg tgcccggtaa tgatgtagtc gtcgccgtcg
     2101 cgccgcgccc gggtggtcat gccgcgcagg tcgctgccgc cagaaggctc ggtcatggcg
     2161 atggcgccga tccactcgcc gctggccatc ctgggcagca cgcgggcctt ctgggcctct
     2221 gacccgtacc gttcgacgta gggcgccacg acatcgttgt gcagcgacat catcggtccg
     2281 accacgccgg ccctgacctg ttcctccatc agggcgacgg agaacaggat gtcggcgttg
     2341 gcgccgccat aggcctccgg agcgcggaag cagagcagac ccagctctcc ggcccttcgc
     2401 cagatggaac gcggcgccat tccggcctct tcccacgcgt cgcgatgcgg cgcgcactcg
     2461 gtctccagga agcgcctggc gctggcccgg aacagttcat gttcgggctt gaagggcagg
     2521 gcgccggcca tggccggcct ggcgatcacg ggagagggcc tgttcacagg gacctgccga
     2581 tcagatcctt catgatctcg ttggtcccgc cggcgatgcg gcccaggcgc gcatcggcgt
     2641 agcggcggga gatcggaaag tccttcatgt agccgtatcc gccaaagacc tgcaggcagt
     2701 cgtcgatgac ctcgcagctg cgttcggtgg tccaccactt ggccatcgcg ccgtcgcggg
     2761 cgtcgaaggt tccctcgatg aagcggtcga cgcagtccgc cagaaaggtc ctggcgaagg
     2821 ccacggtcgt cttgcattcg gcgagcttga agcgcgcgtc ctggtggtcg atcagaggct
     2881 tgccgaacac catgcgctgc cgcgcgtagg cgacggcctc ctcgaccgcg aactccatgt
     2941 tggccacggc ggacatggcc atgatcagcc gttcctcggg caggcgctgc atgagctggt
     3001 agaagcctcg gccctcttcg ccgccgagca ggttctccgc cgggatgcgc acgtcctcga
     3061 agaacagttc ggacgtgtcc tgcgccttgc agccgatctt cctcagattt cgccccagac
     3121 tgaagccctt ggcgtccttc ggctcgagga tcagcaggct gacgccgcgg ccgccggcct
     3181 cggggtcggt cttggcggcg acgacgatga tgtcagcgag ctgagcgttg ctgataaagg
     3241 tcttctggcc gttgagcaca tagtggtcgc cctcgcgcac ggcgcgggtg cggatcgcct
     3301 gcaggtcgct gccggcgcct ggctcggtca tggcgatggc cgaggtgatc tcgccggtca
     3361 ccatgcgcgg cagccaggtc cggcgctgcg cctcggtgcc gtagagctcg atgtacggcg
     3421 cgatgatgtc gctgtgcaga tagaaggcca gctcgttgaa gccggctcgc gtctgctcct
     3481 ccatgatgac catgcagtgg aacaggtcgc ctccgggccc gccgaactcg ggcgcgacct
     3541 ttgaacagag aagcccggcc tcgccggccc tggtccagac gtcgcgcgaa accttcaggt
     3601 ccttttgcca ggccttccgg ttggggaggc actcacgctc gatgaaccgc cgcacgctct
     3661 cgcggaagat ctcatgctca ggggtgtagt agggggactc gatcacatga tctctccaga
     3721 cagacggacc cgcctgaatt tgagcactgc gggccgccgt ccgcgagagg gcggcggccc
     3781 catgcgcctc agaaccgctt gcgcagttcg acaccccagg tacgccccag atcggcgccg
     3841 cgaacggtgt agccgtagat gcccacctgg cggaacgtgc cgccgctcgg ggtgatctcg
     3901 tcggcgatgt tcttgcccca gagataggct ccccaaccgc cggtcgtgga ctggaagccg
     3961 atcctgccgt tgatccgggt ggtggcttca ttggccaggg tgttggccgt gttgacgtag
     4021 gattcgcccc gatagatcac gtcgccccga aggtagaact cgatcgcgtc gttgaccggc
     4081 atgatgaact gggcgccggc gctgccggtg aattcgggca gcccgaagga cttgccctcc
     4141 gacgcggggt cggcggggaa ttcctcgtac ttggcgcgct gcatggcgcc attgaaatca
     4201 agggtcaggt agcgggtcgg gcgcgccgtc aactcgatct cgacgccctt gttcgaggcg
     4261 ctggccgcgt tgcggaccag gaaggacgtg ccgaggtcga tctgctcctg cttgtccttc
     4321 cacgacaggt agaacgccgc gacgttgagg ctcaggcgcc ggtcgaacca gaacgacttg
     4381 tagccggcct cgtagctgtc gacgaactcg ggggcgaagc cgagcgaatc ggccggattg
     4441 aagggcgggg agatcacgtc ggtgttgaaa ccgcccgcct tgaacccgcg gctgtagcgg
     4501 atcgagaaga cctgatcgtc ggagtagtcg tagctcaggc tgatgtcgcc ggtcagctgc
     4561 ccttcgttgt agctctccgc gcgctgggga atgttcaccg cgaagcaggg aatgaacaac
     4621 ccgccggctc cgcaaggacc acccggcccg taggtctgcg tatagtactg atggtaggtg
     4681 aagtccttgt tctcggtcgt gtatcgaagg ccgccggcca gccgcagctg ctcggacagt
     4741 tgataggtgc ccgaggcgaa cagcgcccag gatgtgccct cgatccggga ctcggtccgg
     4801 gccctctccg cgtagccggg gagcgtgagg ccggcgagca gttgggggaa gttggggccg
     4861 gtccggatgc ggcggttgtt ctcggtgttt tccgcgtaga agtagaagcc gccgatccag
     4921 gcgaaggggc cctcgccggt cgaggtcagc cgaagttcct ggctgaagtt ctccccgttt
     4981 tcctcgcggc cgacgttgaa cccgtcgagc tggatgccat cggagtcgtc ctcgaagtcg
     5041 gacatatagc tgcgataggc agtgatcgat gtaagggtga cggagtccag gtcgtagtcg
     5101 acgcggaccg atccgccgcc aagttcgcgc ttggcgaagg agtcggtgtt gagctggacc
     5161 acgttgtcgg acgggtcctg cggaggcacg gtggcgaaga tcggcagggg cacgaggaag
     5221 ctgaccggac cggtctccgc tgcctggctc ccgacctgtt tgaaatagtc cgcgctgagt
     5281 cggatcttca ggcggtcatt gggttgcgag ccgatcacca gtctggcccc gttgcccgag
     5341 aagtcgttca gctcggtgtt gcggaaggtg ttggtgatgt agccttcgcg ttcggcgtgg
     5401 acgaccgaca ggcgcaccat gagaaggtca gggatgatgg ctccgccgac cgtggcccgg
     5461 aactgggaca tgtcatagtt gccgaactgc acgtccccga aggcggtgaa atcgttgctc
     5521 ggatcaccgg tgatcaggtt gatcgcgccg ccgatggtgt tcttgccgta gagggtgccc
     5581 tggggccccc gcagcacttc gatccgctcg atgtcgttga gcgatacgtt gaacgtcgga
     5641 tcgcggccct ggtagacctc gtcgacatag acgccgaccg cgggcggaaa accggccgcg
     5701 tagttgctct gcgaggacac gccgcggatg ctgatcgagg gcgagatcga gccggagctc
     5761 atcagcacgt tggggatctt gaaggcgatg tcggtgacgt tgttggcggt gatgtcctgg
     5821 agctcctcgg cgcccagggc cgtgaccgcg accggcacct tgatgttctc ttcggagcgg
     5881 cgctgcgccg tgatggtgat cgactcgacc gtatagtcgc cgctgttgga cggcgcggtc
     5941 ttgaccggag cgtcctgggc gaacgcgggc gcggccatca gggccgcggc cgaaacacct
     6001 attcccagca tggcgcgacg cgcgacctgg cgcgagagct tgtcgttcat gggtttcctc
     6061 cccccgtttg cggacagctg tcgcggcctt tccccaactt cagacgtggg gtgagccgtc
     6121 ctgtccgtcc ttgagacaca gtcccgtcgc aaacccgcca gtcacgcgtc gcgccgacgg
     6181 aaacggcgga cgacctgcga accggccgcc accagcatgg cgccgatggc cagccagccc
     6241 atccagtccc ccagccgggt ggtgacggtc gggcccgtgg ccaggggcac gtcggcgatc
     6301 agcgtctgcg cggccagttc gcgcgtggcg gtcacgcgtc tggcctccgc catgctcagc
     6361 ggattgacgt tgcgggccag caggcggccg tagggatcga caatggcggt gtcgaacagg
     6421 ctgtcggcct tggccatcgg caggcggttc tcgatcgccc ggaacaccag gtgtgtgtag
     6481 tgggtggtcg cgatcgccgg cacgtcattg gacggtacgg cgatgacgcc cgcgccgcga
     6541 cgggccatct cgcgggcggt gtcggtgaag tccaggtcgt agcagatgat ggttgcgacc
     6601 ttgcccaggg ctgtgtcgta gaccttgtag tcgcctccgg tgtcgctctg gtcccctgcg
     6661 aaccggccgg ggtgcgactt gccataaggg ccgaagacct ggccgtcggg cgcgaagagc
     6721 accgcttcat tgagccgcag gccgtcgggc tgcttcagac cgtagccgag cgcgatgtgg
     6781 gcgccagtct cggccgccag cgccttgagc tccgcggtcc gctcccgcag cggatgaaac
     6841 ttcagcatgc cttcgcgcca gacgaccagc ttcgccccct gcgcggccag ggcgcgggtg
     6901 gcggcgacat tgcgggccag ctcctcctcc ggatcccggg gggcgttggc ctggacggtt
     6961 ccgacccgca gggtggcggg atggttctgg aactggacca gcgacatgat cgtccaggcc
     7021 acagccaggg cgccgacgac tccggcggcg cgcagggccc gcgccattgg caggaccggc
     7081 gcaccctctg gcgcgcgtct gcgatcgagc agggcgatca ccagtccggc gaccgcgaag
     7141 ttgatcacca gcaacaacag ctccagcccg aacaggccga acaccgtcaa cggctgcagt
     7201 agccagggct gggcatagag ggcgtagacc ggattgcccc aggtgccggc cagcacgtcc
     7261 acgccggaca gggcccgtgc ggcgtcgatg ccgacccagg cgacggggaa ggacagcacc
     7321 agccagcggt aggcggtcct gctgtggaaa tggcggccgg gccagcacag ggccgcgatg
     7381 agcagaccga catagagcgg ccagagctgg atgtaccagg cgacgccccc gtcggagaga
     7441 ccagccgaca gctggccggc gtagtaggcg ccgatcgccg cgccgggcgc gagcggtccc
     7501 agacccttcg gcagaatccg gtgctgggcg accaccatcg gcacgaaggc cacgaagatc
     7561 agcggccaca gctcgaacgg cggaaaggcg agggtggcca ggcctccgct ggcgagccca
     7621 agggccacgc cggccgcggt gcgcatcatc gggcgcgccg ccggcgcgg
//
LOCUS       DAORNX010000093         7645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7085_length_7645_cov_6.957049, whole genome shotgun sequence.
ACCESSION   DAORNX010000093 DAORNX010000000
VERSION     DAORNX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_7645_cov_6.957049"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLO65_05725"
     CDS             <1..697
                     /locus_tag="PLO65_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27780.1"
                     /translation="TLAAEGANVAVCARNAEQVAEAVTALGGAGVKATGAAVDILDGE
                     ALKRWIEAAGAELGGIDILVSNAGAMAQGADPASWDQNFRLDVLGMVNAFDAARPFLL
                     KAAETHGDASFVIISSVSAAETDSASSYGPIKAALIHFAKGLARQYAPRHVRANVVSP
                     GTVYFEGGVWQTIETNLPEVFKGAMARNPMGRMATPQDIANAAVFLSSPLSSFTSGVN
                     LLVDGALSRRVNF"
     gene            749..1252
                     /locus_tag="PLO65_05730"
     CDS             749..1252
                     /locus_tag="PLO65_05730"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HRD27781.1"
                     /translation="MDKRQTSVSDPFSQQLVDQRRRRRSLTRRWAALSWSDRALLVEA
                     TAMVTFGALAVRLLPFRRVVTLASGRGGPPSGRSDWAGAAARVAWAVRAAGRRAPFRV
                     VCFQEGLAAHWMLRRRGAPSQLHYGAALDRARGLSSHVWVTVGETDVVGCELAGDYRL
                     LASFPPG"
     assembly_gap    1260..1360
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1361..2537
                     /locus_tag="PLO65_05735"
     CDS             <1361..2537
                     /locus_tag="PLO65_05735"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD27782.1"
                     /translation="ELRLLVACTAWPPGPARTTAITAAVDDPALDWERVLIAVDRHRV
                     AGFVHHGLTAAAAGGAPATREAIRGRAAGDALLNLRLLGEALRLVALLTEAAIPVQVL
                     KGPGLMVEAYGDLTVRQSRDLDLLVPLEHVRRAADICERAGYAPLPPTAHEPDPAMSL
                     WLAWKKDFVFLAPATGVLLELHFRPTYSPLLSDRLDLLAETREIAVAPGRTAPVPTRD
                     ALYAYLCVHGARHAWFRLKWLGDIAALTAGMPDPEIADLHAMASARGAGRASGQALLL
                     LEAVYGRSLAAALRRELLGDPRVARLFRFGLRAITDPREPSTVPGASLKFFLHGLLLD
                     NSWAFRMHETRAWLLLWPLIDAVPLPRPLWFLYLLLAGPLRLTEKVAGLMRIGDHRS"
     gene            2537..3508
                     /locus_tag="PLO65_05740"
     CDS             2537..3508
                     /locus_tag="PLO65_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27783.1"
                     /translation="MAPTPLPEHPLYEFVNVLVRSQMPLPELTTATPRAEDDGDRREV
                     IDIGFGPLPEALPDARTIAHGLQVAGDSVLLTVEDTARYLVRGGREIVIDPIPGGSER
                     NLRLFLLGSALGILCHQRGLLPLHANAIVVDGQAIAFAGHSGAGKSTLAAHFERAGYE
                     ILCDDVCALSFDGDGRPFAWPGLPRLKLWGDAAEAFGHHRDNLDQAIEGMDKYHVPLT
                     GRARARPVPFRRLYLLRQAEDGAGDVTRLRGRDAMETVMAQTYRGQYLDPMGLRARHF
                     GQCAALLAHAEVFTASRVWGYDVFDAEARRLERHLLDADPSDWGSRK"
     gene            3505..3807
                     /locus_tag="PLO65_05745"
     CDS             3505..3807
                     /locus_tag="PLO65_05745"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HRD27784.1"
                     /translation="MKTQSALAGGDIVVRDEGLLTAEVDGELVAMSIDRGVCYGLNSV
                     GSRIWALLAEPRSIDDLCRQLLSEFDVGPEDCRDQVMALLEDLRAEAMVTVSSAAG"
     gene            3910..4062
                     /locus_tag="PLO65_05750"
     CDS             3910..4062
                     /locus_tag="PLO65_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27785.1"
                     /translation="MDHDDYHATAPAAAPAMTDALAAWEKPLLRRLDAGDAEAIVIGV
                     GVDLGV"
     gene            4673..4831
                     /locus_tag="PLO65_05755"
     CDS             4673..4831
                     /locus_tag="PLO65_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27786.1"
                     /translation="MRDDDKGAIAADGHIEAATDLATWERPELRRLDAADAEAAVGFA
                     ATDAGIYS"
     gene            4938..6878
                     /locus_tag="PLO65_05760"
     CDS             4938..6878
                     /locus_tag="PLO65_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRD27787.1"
                     /translation="MSALAGLWNFDGAPTAGESCQRMLTAQAIYGPDAEAIRDLGDVA
                     LGRRLFITVPEDVHDRQPLAGGDGRFALVADLRLDNRDELARDLGIPAPDAARHSDAD
                     LLLAAWERWEDRVFEHLLGDYAFAIWDARERRLVLARDPCGARPLHFHRGAGFFAFAS
                     MPKGLHALPDIPYGPDEVRMAEFLALLPETGPRSFFKDIQRVESGQVVVATTSGTSSR
                     RHWTPSRKTLARGRADDYAEGVRHHLDRAVQVRLRRSAGPVGTHLSAGLDSAAVAATA
                     ARLLAPRGEGPVAFTSVPRKGYEGEPPPGRFGDETGLARATAALYPNMDHVLVRTGDR
                     GVMDDFDRNFFLFERPVVNACNQLWINAINAQAQKRGVRVLLTGAVGNLTLSYAGREL
                     LPELMAAGRWLRLFREGRAMVRAGEARWRGVLAQSLGPWIPQRLWLQLQRLAGGHVGD
                     LASYSMLSVDRQREIALDSRAADLGFDLAYRPQTSAFDARLGALRRVDSGNQNKGALA
                     GWGVDLRDPTGDQRLVEYCLSLPTDAFMEEGRPRALVRRALADRLPEVVLSERRKGLQ
                     AIDWHESMTASRDQIADEVARLAEVPAASTALNLPRMRQLVENWPTGNWNSRAVSTTY
                     RLALLRGVANGHFLRKASRSNA"
     gene            complement(6879..>7645)
                     /locus_tag="PLO65_05765"
     CDS             complement(6879..>7645)
                     /locus_tag="PLO65_05765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD27788.1"
                     /translation="IGSGTGLLAMMAARAGAAAVVTCEMNPAVADAATDIVALNGYAD
                     RVRVIAKRSTDLDPDADMGGRADVLVSEIVSNDLLREGALPVMEDAVARLLKPGGRMI
                     PESGRIRVALAHWAGLDRRRMSLVAGFDMTPFNRLARRPERLEVGDPELALRGPAADL
                     FEFDFSSGGPYRPGRATVNLVADGQAINGVAQWISLRMDAAGDYENQPAAGASSCWAC
                     LFHPFERELRPLAGTAMSIHGAHTRQALRIWSGGTA"
BASE COUNT         1069 a         2627 c         2686 g         1162 t
ORIGIN      
        1 gaccctggcg gcggaaggcg ccaacgtcgc cgtctgcgcc cgcaacgccg agcaggtcgc
       61 cgaggcggtg acggccctgg gcggcgccgg cgtcaaggcc acgggcgcgg cggtggacat
      121 cctcgacggc gaggcgctga aacggtggat cgaagccgcc ggcgccgaac tgggcggcat
      181 cgacatcctc gtgtccaacg ccggggccat ggcccagggc gccgacccgg cctcgtggga
      241 ccagaacttc cgcctcgacg tgctgggcat ggtcaacgcc ttcgacgccg cccggccgtt
      301 tctgctgaag gcggccgaaa cccatggcga cgcctcgttc gtgatcattt cctcggtgtc
      361 ggcggcggag accgacagcg ccagctccta cggcccgatc aaggccgcgt tgatccactt
      421 cgccaagggc ctggcccggc agtacgcgcc ccgccatgtg cgcgccaacg tcgtctcgcc
      481 cgggacggtc tacttcgagg gcggcgtctg gcagaccatc gagaccaacc tgcccgaggt
      541 gttcaagggc gcgatggccc gcaatcccat gggccgcatg gccacgccgc aggacatcgc
      601 caacgcggcc gtattcctga gcagcccgct gtcgagcttc accagcggcg tcaatctgct
      661 cgtcgacggc gccctcagcc gacgggtgaa cttctaggcc ggcggagcgg cccccgtcgg
      721 cgcggccatg ccggggagag aagtccagat ggacaagaga cagaccagcg tgagtgaccc
      781 attctcgcag caattggtcg accagcgtcg acgtcgacgc tcgctgaccc ggcgctgggc
      841 ggccctgtcc tggagcgatc gcgccctgct cgtcgaggcg acggccatgg tcacgttcgg
      901 ggcgctggcc gtgcgtctgc tgccgttcag acgggtcgtg accctggcgt cgggacgcgg
      961 gggtccgccg tccggccggt cggactgggc cggggcggcg gcccgtgtcg cctgggccgt
     1021 acgcgccgcc ggccggcgcg cgccctttcg cgtcgtgtgc tttcaggagg gactcgccgc
     1081 ccactggatg ctccggcggc gcggggcgcc ctcgcagctt cactatggcg cggcgctcga
     1141 tcgggcccgc gggctgtcgt cccatgtctg ggtcaccgtc ggcgagaccg atgtcgtcgg
     1201 gtgcgaactg gccggcgact atcgcctcct ggcctccttc cccccgggct gacggcaggn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggagctgaga ctgctggtcg
     1381 cctgcaccgc ctggccgccc ggccccgcga ggaccacggc catcacggcc gcggtcgacg
     1441 acccggcgct ggactgggag cgggtgctga tcgccgtgga ccgtcaccgg gtcgccggct
     1501 tcgtccatca tggcctgacc gccgccgcgg ctggaggcgc tccggcgaca cgcgaggcca
     1561 ttcgtggccg ggcggccggc gacgccctgc tgaacctgcg cctgctcggc gaggccctgc
     1621 gcctcgtcgc cctgctcacc gaggccgcga tcccggtcca ggtgctcaag gggccgggcc
     1681 tgatggtcga ggcctatggc gacctgaccg tgcgccagag tcgcgacctg gacctgctgg
     1741 ttccgctcga gcatgtccgg cgggcggcgg acatctgcga gcgggccggg tacgctcccc
     1801 tgccgccgac ggctcacgag ccggacccgg ccatgtcgct ctggctggcg tggaagaagg
     1861 acttcgtctt cctggccccg gccaccggcg tcctgctgga gctccatttc cggcccacct
     1921 acagccctct tctgtcggac cgactcgacc tgctggccga gacccgggag atcgccgtgg
     1981 ccccggggcg aacagcgccg gtgcccacgc gggacgccct ctatgcctac ctctgtgtgc
     2041 atggggcccg gcacgcctgg ttccggctga agtggctcgg tgacatcgcc gccctgacgg
     2101 cgggcatgcc tgacccggaa atcgccgacc tccacgccat ggccagcgcg cggggcgcgg
     2161 ggcgggcgtc gggccaggcg ctgctgttgc tggaggccgt ctatggacgg tccctggcgg
     2221 cggcgctgcg gcgcgaattg ctgggcgacc cccgggtggc gcggttgttc cgcttcgggc
     2281 tccgggcgat caccgacccg cgtgagccga gcaccgtccc cggcgcttcg ttgaagtttt
     2341 ttctgcacgg gctgttgcta gacaacagct gggcgtttcg catgcatgag acacgcgcgt
     2401 ggctgctgtt gtggccgctg atcgacgccg tgccgcttcc ccgcccgtta tggttcctgt
     2461 atcttctgct ggccggtccc ttgcggctca cagagaaggt agcgggcctg atgcggatcg
     2521 gcgatcatcg gtcgtgatgg cgccgactcc gctgcccgag catccgctct acgaattcgt
     2581 caatgtgctg gtgcgctcgc agatgccgct cccggaactg acgacggcga cgccgcgcgc
     2641 cgaagacgac ggcgaccggc gcgaggtcat cgacatcggg ttcggtccgc tgccggaggc
     2701 gctgccggac gcccggacga tcgcacacgg cctgcaggtg gccggcgaca gcgtgctcct
     2761 gacggtcgag gacactgccc gctatctggt cagggggggg cgggagatcg tcatcgaccc
     2821 gatccccggc ggttcggagc gtaacctgcg cctgttcctg ctcggatcgg cgctcgggat
     2881 cctctgccat cagcgcggcc tgttgccgtt gcacgccaac gccattgtcg tcgacggcca
     2941 ggcgatcgcc ttcgccggcc attccggcgc aggaaagtcg accctggcgg cccatttcga
     3001 gcgcgccggc tacgagatcc tttgcgacga cgtctgcgcc ctcagctttg acggagacgg
     3061 ccggccgttc gcctggcccg gcttgccgcg cttgaagctg tggggcgacg cggccgaggc
     3121 gttcggccat catcgcgaca acctcgacca ggccatcgag ggcatggaca agtaccatgt
     3181 gccgctgacc gggcgggcgc gggcgcggcc ggtgccgttc cgccgtctct acctgctgcg
     3241 gcaggccgag gacggcgccg gcgacgtcac gcggttgcgg ggacgcgacg cgatggagac
     3301 ggtgatggcc cagacctacc gtggccagta tctcgatccg atggggctga gggcgcgaca
     3361 cttcggccag tgcgccgccc tgttggcgca cgccgaggtt tttaccgcat cgcgggtgtg
     3421 gggttatgat gtgttcgacg cggaggcgcg gcggctcgaa cgccacctgc tcgacgccga
     3481 tccatcagat tggggaagcc ggaaatgaag acgcaatccg ccctcgccgg cggcgacatc
     3541 gtggtccgcg acgagggcct gctgaccgcg gaggtcgatg gcgagctcgt cgccatgagc
     3601 atcgaccggg gcgtctgcta cggcctcaac agcgtgggat cacggatctg ggcgctgctc
     3661 gccgagccgc ggtcgatcga cgacctctgc cgccagttgc tgtccgagtt cgatgtcggg
     3721 cccgaggatt gccgcgacca ggtgatggcg cttctcgagg atcttcgcgc cgaggccatg
     3781 gtcacggtga gttccgcggc gggctgacga cgggacagtc cgccgggcga actcgattgc
     3841 ggcccgctgg ggattgtgct gaggcattgc cgttgagggc cataaggccc ccgcgccgag
     3901 gggaaagcca tggatcacga tgactaccac gccaccgcgc ctgccgctgc gccggccatg
     3961 acggacgcgc tcgccgcgtg ggaaaagccg ctcctgcgca ggctcgacgc cggcgatgct
     4021 gaagccatcg tgatcggcgt gggtgttgat cttggggtct aatcctgaaa ctgcgcgcgg
     4081 cgcccgttga tcgttgatcg agaaacgcca aagtccggga ggggtcggtt cgccggattc
     4141 cggttcgggg gaatggccac ccggttcctc gggtttgagc gaccgtctgc gacgatcgcc
     4201 ggcagcgatt ccggaccggg gcggagccgc ccggcagccc tgattgcgat ccgcgacgga
     4261 ctgcgtccat acctgaaccg ggcgccggga ccgggggcgg cgcgcggggt tgcggtatcg
     4321 accgggcgag gcgcctgtgg ctaacttgac gcgccgaatc acacgccggg gagcgcaagg
     4381 tgcagtcata cgaagaagcc gggtcatcgc ccgggcttga ggtcaagccg acgacgatcc
     4441 gcgccccatg ggagtcgccc cacctgcgtc gtcttgacgc cggggatgcg gaagcccggg
     4501 ttcttcccga ttttccgctg cgccgacttc tgggagcctc ataggccgga gccggccggc
     4561 agccgcgccc gcgaattcgc cgacgaggcg agggccggtt gcagcaagga tttattatgt
     4621 tttgctgcca agttcaggct aggatcgatt caacctgtgt aaggagatga ccatgcgtga
     4681 cgacgacaag ggcgccatcg ccgcggacgg ccatatcgag gctgcgaccg atctggcgac
     4741 ctgggagcgc ccggaactgc ggcgcctcga cgccgccgac gccgaagccg ccgtgggctt
     4801 cgccgcgacg gatgccggca tctattcctg acgacgtccg gcccggcggt cgccgggcga
     4861 cagtccttca agcgcaagac ggctcgccgc cggcgggccg ttgttgcgcg tctgtatttg
     4921 tgagatgcgc cggatagatg tccgccctgg ccggtctctg gaacttcgac ggcgccccga
     4981 ccgcgggcga gtcctgccaa cggatgctga cggcccaggc gatctatggt cctgacgcgg
     5041 aagccattcg ggatctgggc gacgtcgccc tcggccgtcg cctcttcata accgttcccg
     5101 aggacgtcca cgaccgccag ccgctggccg gcggagacgg tcggttcgcg ctggtcgcgg
     5161 acctcaggct ggacaaccgc gacgaactcg cgcgcgacct cggcatcccg gcgcccgacg
     5221 cggcccggca ttctgacgca gacctgctgc tggcggcctg ggagcgctgg gaggacaggg
     5281 tcttcgagca cctgctgggg gactatgcct tcgcgatctg ggacgcccgc gagcgacggc
     5341 ttgtgctggc ccgggatccg tgcggcgcga ggccgctgca cttccatcgg ggggccggat
     5401 tcttcgcctt cgcctcgatg ccgaagggac ttcatgccct gcccgacatt ccctacgggc
     5461 ccgacgaggt gcgcatggcg gagtttctcg ccctgctgcc agaaacgggt ccgaggagct
     5521 tcttcaagga catccagcgg gtggagtccg gccaggtcgt ggtggccacg acctccggga
     5581 cgtcgagccg gcgacactgg acgccctcgc gaaagaccct ggcccggggc cgggcggacg
     5641 actacgccga gggcgtccgg caccatctcg accgggcggt ccaggtccgc ttgcgtcgga
     5701 gcgccggtcc ggtcggaacg catctgagcg caggtctgga cagcgcggcc gtcgcggcta
     5761 ccgcggcgag gctgctggcg ccccggggcg agggaccggt cgcgttcacg tcggttccac
     5821 gcaagggcta cgagggcgag ccgcccccgg ggcggttcgg ggacgaaacc ggtctggccc
     5881 gggccacggc ggcgctctac ccgaacatgg accatgtgct ggtccgaacc ggcgaccgcg
     5941 gcgtcatgga tgacttcgac cgcaacttct tcctgttcga gcggccggtg gtcaacgcct
     6001 gcaaccagct gtggatcaac gcgatcaacg cccaggcgca gaagcgggga gtccgggtgc
     6061 tgctgaccgg cgcggtcggc aacctgaccc tcagctacgc cggccgcgaa ctgctcccgg
     6121 aactgatggc ggcggggcgc tggctgcggc tgttccggga agggcgggcc atggtcaggg
     6181 ccggcgaggc ccgctggcgc ggcgtgctgg cccagagcct gggtccgtgg atcccgcagc
     6241 gactctggct gcaactgcag cggctcgccg gcggtcatgt cggcgacctg gccagctact
     6301 ccatgctcag tgtggatcgt cagcgcgaga tcgcgctgga cagccgggcc gctgaccttg
     6361 gcttcgacct ggcctaccgg ccacaaacct ccgccttcga cgcccggttg ggcgccctgc
     6421 ggcgcgtgga cagcggcaat cagaacaagg gggcgctggc aggctggggc gtggacctga
     6481 gggacccgac cggcgaccag cggctggtag aatactgcct gtccctgccg accgacgcct
     6541 tcatggagga aggacgaccg cgcgctctcg tccggcgggc cctggcggac cgtttgccgg
     6601 aggtcgtcct gtcggagcga cgcaagggcc tgcaggcgat cgactggcac gagagcatga
     6661 ccgcctcccg cgaccagatc gccgacgaag tcgcgcgcct cgcggaggtc ccggccgcgt
     6721 caacggcgct caacctgcca cggatgcgac agttggtcga aaactggccg accggaaact
     6781 ggaactcgcg ggccgtttcc acgacctatc ggctggcgct gctccgcggc gtcgccaacg
     6841 gccatttcct gcgcaaggcc tcgcgcagca acgcctgatc aggccgtgcc gccagaccag
     6901 atccgcaagg cctggcgggt gtgcgcgccg tggatggaca tcgccgttcc cgcgagcggg
     6961 cgcagttccc gttcgaacgg gtggaacagg caggcccagc atgacgacgc gccggcggcc
     7021 ggctggttct catagtctcc cgccgcgtcc atccgcaggg agatccactg cgccacgccg
     7081 ttgatggcct ggccatcggc gacgaggttc acggtcgccc gaccgggccg atagggcccg
     7141 ccggacgaga aatcgaattc aaacagatcc gcggccggcc ccctcagcgc caactccggg
     7201 tcgccgacct ccaggcgctc gggccggcgg gccaggcgat tgaagggcgt catgtcgaaa
     7261 ccggccacca ggctcatccg tcgccggtca agccccgccc agtgggccag cgccaccctt
     7321 atgcggcccg actccgggat catccggccg cccggcttga gcaaccgggc cacggcgtcc
     7381 tccatcaccg gcaacgcgcc ctcccgcaga aggtcgttgg aaacgatctc cgacaccagc
     7441 acatcggccc gtccccccat gtccgcgtcg ggatcgaggt cggtggagcg cttggcgatc
     7501 acccggacac ggtcggcgta gccgttgagc gccacgatgt ccgtggcggc gtcggccacg
     7561 gccggattca tctcgcaggt gaccaccgcc gccgcgccgg cccgggccgc catcatcgcc
     7621 agcaggccgg tgcccgagcc gatgt
//
LOCUS       DAORNX010000094         7589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7152_length_7589_cov_8.071410, whole genome shotgun sequence.
ACCESSION   DAORNX010000094 DAORNX010000000
VERSION     DAORNX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7152_length_7589_cov_8.071410"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..533
                     /gene="vsr"
                     /locus_tag="PLO65_05770"
     CDS             126..533
                     /gene="vsr"
                     /locus_tag="PLO65_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HRD27789.1"
                     /translation="MTDVYDSAKRSAVMRRVKSRDTTPEKTVRRLLTGLGARYRLHRR
                     DLPGRPDIVMAGRRLAIFVHGCFWHGHDCPRGGRVPRANRDYWLAKVGRNRERDIASR
                     AALEAAGWRVEAIWECEMKDEAALRERLARMLA"
     gene            complement(570..>792)
                     /locus_tag="PLO65_05775"
     CDS             complement(570..>792)
                     /locus_tag="PLO65_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD27790.1"
                     /translation="DMDGREATRRLRAQPGPNQAAPVIAITASATERDWEACRAAGMT
                     GHVAKPIEPAQLYAALDAALAEIEAREAA"
     assembly_gap    793..823
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<824..2291)
                     /locus_tag="PLO65_05780"
     CDS             complement(<824..2291)
                     /locus_tag="PLO65_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27791.1"
                     /translation="MEQTDRFGIRASATARRRHLKMRIVTALIVGAVLHFALGWWLTW
                     VWSAAYVGTQLLEAWATPRLLRNSVDTPAEAANIAVVLFFPGALVFGGLAVALWISVP
                     GYGPALGVAVIASAMINLIALSRGSRLAFAASATPYGLYLLVMPMLDYGRIAGPLLTT
                     MMIAVGLIMLNIIGAWISTEEARRAHQMATEEADRRRTEAEAAVEAKSAYLAMISHEL
                     RTPISAILAGAGELERTAPGQGRAQARLIRGAGQMMRGLLDDLLDLSKIEVGRMEVES
                     VAFDLRAVVCEALKMWRPEARHKGLRLRVEGAHTLPEWVQGDPTRIRQVLNNLISNAL
                     KFTAQGSITLRLAAEPVGDGDQRAISLAVADTGPGMSPAQVARLFTPFDQLDAGTARK
                     HGGSGLGLAISRELARLMGGELTAASAPGEGAVFTLNLTLPTIAAPAGASSPALVEAR
                     VLVIDDHAVNRQAIALVLAPLGITPETAASAEEGLERLA"
     gene            complement(2426..2896)
                     /locus_tag="PLO65_05785"
     CDS             complement(2426..2896)
                     /locus_tag="PLO65_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRD27792.1"
                     /translation="MAHVATIDWAGKPGEDFAAGHYSRAHDVIFDGGITVPGSASPSV
                     VRAPWSVEAAADPEEMLIAALSSCHMLTFLDKARRAGFVISRYRDEAEGVMRKTPEGR
                     IAVTRVALRPRITFEGRQPTDQELDALHHAAHEECFIANSVRTEVVVEPPPPPP"
     gene            complement(2896..3276)
                     /locus_tag="PLO65_05790"
     CDS             complement(2896..3276)
                     /locus_tag="PLO65_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27793.1"
                     /translation="MIDHLSIAVSDLVRSAAFYDAVLGPLGYRRLVDRPGTVGFGKRY
                     PEVWLNHRPGVTRADPDTGHHVCLRASSPEAVEAFHTAALAHGGADAGAPGPRQAAMT
                     TYIGAFILDPDGAKLEAACFPREG"
     gene            complement(3283..4446)
                     /locus_tag="PLO65_05795"
     CDS             complement(3283..4446)
                     /locus_tag="PLO65_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD27794.1"
                     /translation="MGVLNLDRTPSLAEEDIVIFEDSVGRFFDEKAPESRVAKWREDG
                     KVERLFWNEAAEAGLSCLSIPEEYGGMGGDYRHEVILMEQLGAKGVDGFGLSLHNAIV
                     APYILHCGTEEQKRRWLPKLASCEYVSAIAMTEPGAGSDLQGIKTTARLDGDAYVING
                     SKTFITNGQTANFIIVVCKTDPTQGARGTSLIVVETDNAPGFERGRNLDKLGQEAADT
                     SELFFNDVRVPAANLLGGGEGQGFFQLMGQLPQERLNIAVQAIATIERALALTIDYVK
                     QREAFGKKVIDFQNTQFVLAECKTEATVARVFVDHCIEAHLKGKLDAATASMAKYWLT
                     DLENKIIDRCLQLFGGFGYMAEYPIARMYRDSRVQRIYGGTNEIMKVLIARTL"
     gene            complement(4446..6236)
                     /locus_tag="PLO65_05800"
     CDS             complement(4446..6236)
                     /locus_tag="PLO65_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRD27795.1"
                     /translation="MAYQPPARDHVFLLRDVLEIDRYSSLPAFADASMDVVEQIIEEA
                     AKFTAGVLAPLNAVGDKQGCQWSPDFTVKTPAGFREAYQQLCAGGWTGLGSDPAYGGQ
                     GLPHVVNLSFSEMSSSANMAFSMYPGLAHGAYSAIHTGGSDAQKQTYLPKMVSGEWTG
                     TMNLTEPHCGTDLGLLRTKAVPEADGSYRISGQKIWISAGEHDLADNIVHLVLARVEG
                     APAGVKGISLFIVPKFLPNPDGSVGPRNEGVKCVGLEEKMGIHGNATCVMQYEDAKGW
                     LIGEENSGLKLMFVMMNEARIGVGLQGVAQGEAAYQAARDFAKDRLQGRSLTGPKNPE
                     GPADPIIVHPDVRRMLLDSRAVIEGGRAFLFWTALHGDLSHGHPDEAVRQKAGDYMGL
                     LTPVLKGYLTDKGFKACVDGMQVHGGSGFTEHFPASQYLRDCRIALIYEGTNGIQALD
                     LVGRKLAANGGRAVMSWFAEIDGFVEENAGDEKLKPFLDGLASAKAQLQDGTMWLMQN
                     GLQNPDNAGAASTDYMHLFGVTGLAYMWALMAKVSLAKIAAGDTDPFYASKVSVGQYF
                     LERILPDAGAHLAKLKTGSANMMSIPAEAF"
     gene            complement(6279..6761)
                     /locus_tag="PLO65_05805"
     CDS             complement(6279..6761)
                     /locus_tag="PLO65_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRD27796.1"
                     /translation="MPAELRRPERTFTIRQLCTEFKATPRALRFYEDKGLLTPARDGM
                     NRVYSYKDRARLILILRGKRVGLSLQEIREILELYGEHDEAMTQNAHSLKKFREKIVD
                     LEQQREDIEGAINELREACERLEKHLAAKRPDLLPKAADYDQMLRARLDGHPEMLGAK
                     "
     gene            6931..7257
                     /locus_tag="PLO65_05810"
     CDS             6931..7257
                     /locus_tag="PLO65_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27797.1"
                     /translation="MDSLPVASAVARALVTTESAVDEALVRAVALMRRMISGRRELGL
                     PVALTDPVMGRVSAAVNALGDAHRELVRAHGDLARIGAELGLGDLGFGPLVKPTGGPA
                     EPQPVD"
     gene            7261..>7589
                     /locus_tag="PLO65_05815"
     CDS             7261..>7589
                     /locus_tag="PLO65_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27798.1"
                     /translation="MALLLFALLLAIAATGALVRGGLTERRGAALFTSAWIVSLAAQW
                     VSGRAAPGLWLPVIDAAVLVGLAALAWRSPRPWPVYASGFQLLTLTGDIARWVETDLD
                     VRIHLTLL"
BASE COUNT         1062 a         2530 c         2691 g         1275 t
ORIGIN      
        1 gtctgggccg gcctgcggcg atggctgttc accccgctgt cgcgtgcgcc gggcgttcgc
       61 cagctcgggg cggcgatcct gaccggcacg gcgcggctgg gccgctggcc ggacgggacc
      121 cggccgtgac cgacgtctat gattccgcca agcgttcggc ggtgatgcgc cgggtgaaga
      181 gtcgcgacac gacgcccgag aagaccgtgc gccgcctcct gaccggactt ggcgcccgct
      241 accgcctgca ccgcagggac ctgcccggcc gacccgacat cgtcatggcc ggccggcggc
      301 tggcgatctt cgtccacggc tgcttctggc acggtcacga ctgtccgcgc ggcggccgcg
      361 tgcccagagc gaaccgtgac tactggctgg cgaaggtggg ccgcaaccgt gaacgggaca
      421 tcgccagccg cgcggcgctc gaggcggccg gctggcgggt cgaggcgatc tgggagtgcg
      481 agatgaagga cgaggccgcg ctgcgggagc ggctggcgcg gatgctggcc tgaggggacg
      541 cgtcccttcg gcacacatcc ccttcggagt caggccgcct cccgcgcctc gatctcggcc
      601 agcgcggcgt cgagcgcggc gtagagctgg gccggctcga tcggcttggc gacatgaccg
      661 gtcatgccgg ccgcccggca ggcttcccag tcccgctcgg tggccgaggc ggtgatggcg
      721 atcaccgggg cggcctggtt cgggccgggc tgcgcgcgca ggcgacgggt cgcctcgcgg
      781 ccgtccatgt cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggccag ccgctcgaga
      841 ccctcctccg ccgaggcggc ggtctccggc gtgatgccca ggggcgcgag aaccagcgcg
      901 atggcctggc ggttgacggc gtggtcgtcg atcaccagca cccgcgcctc gaccagggcg
      961 ggggacgagg cgccggccgg cgcggcgatg gtcgggaggg tcagattgag ggtgaacacc
     1021 gccccctccc cgggcgcgct ggcggcggtc agttcgccgc ccatcagccg ggcgagctcg
     1081 cggctgatgg ccaggcccag acccgagccg ccgtgcttgc gggccgtgcc cgcgtcgagc
     1141 tggtcgaagg gggtgaacag gcgggcgacc tgggccgggg acatccccgg gccggtgtcg
     1201 gccacggcca gggatatggc ccgctgatcg ccatcgccga ccggttcggc ggccaggcgc
     1261 agggtgatcg agccctgggc ggtgaatttc agggcgttcg agatcaggtt gttgagcacc
     1321 tggcgaatgc gcgtcggatc gccctgcacc cattccggca gggtatgggc gccctcgacc
     1381 cgcagccgca gtcccttgtg acgcgcctcg ggccgccaca tcttcagggc ctcgcagacg
     1441 accgcccgca ggtcgaaggc gacgctctcg acctccatcc ggccgacctc gatcttggac
     1501 aggtcgagca ggtcgtcgag caggccgcgc atcatctggc cggccccgcg gatcagccgg
     1561 gcctgggcgc ggccctgccc tggcgcggtg cgttccagct cgccggcccc ggcgaggatg
     1621 gcgctgatcg gcgtgcgcag ttcgtggctg atcatcgcca gataggccga cttggcctcg
     1681 acggcggcct cggcctcggt gcgccggcgg tcagcctcct cggtggccat ctggtgggcg
     1741 cgacgggcct cctcggtgct gatccaggcg ccgatgatgt tgagcatgat caggcccacc
     1801 gcgatcatca tggtggtcag cagcgggccg gcgatgcggc catagtcgag catcggcatg
     1861 accagcaggt acaggccata gggcgtcgcc gaggcggcga aggcgagccg cgagccgcga
     1921 ctcagggcga tcaggttgat catggcgctg gcgatcaccg ccacgccgag cgcgggaccg
     1981 tagccgggca cgctgatcca cagggcgacc gccagcccgc cgaacaccag ggcgccgggg
     2041 aagaacagca ccacggcgat gttggcggcc tcggccgggg tgtcgaccga attgcgcagc
     2101 agccggggcg tcgcccaggc ttccagcagc tgggtgccga cataggccgc cgaccagacc
     2161 caggtcagcc accagccgag cgcgaaatgc aggacggcgc cgacgatgag ggcggtgacg
     2221 atgcgcatct tgagatggcg gcggcgggcc gtcgccgacg cccggattcc gaacctgtcc
     2281 gtctgttcca tgccgttcat cgcgccgtcg ttccccggtc tttggcggac cttgcgggag
     2341 gtcttcctaa gactcagtga aagaacgacg gtctttcaga tggttagcag ggcgtgaacg
     2401 gcccggcgca aggggccgtc gcgcgctatg gcggcggcgg cggcggctcg acgacgacct
     2461 cggtcctgac cgagttcgcg atgaagcact cctcgtgcgc ggcatggtgc agggcgtcga
     2521 gttcctggtc cgtgggctga cgcccctcga aggtgatccg cgggcgcagc gcgacccgcg
     2581 tcacggcgat cctgccttcg ggcgtcttgc gcatcacgcc ttcggcctcg tcgcgatagc
     2641 ggctgatgac gaagccggcc cgccgcgcct tgtcgagaaa ggtcagcatg tgacaggacg
     2701 acagggccgc gatcagcatt tcctcgggat cggcggccgc ctcgaccgac cagggcgccc
     2761 ggaccacgga gggcgaggcc gagccgggca cggtgatgcc gccatcgaag atgacgtcat
     2821 gcgcgcggct gtagtggccg gccgcgaaat cctcgccggg cttgccggcc cagtcgatgg
     2881 tggcgacgtg ggccatcagc cctcccttgg gaaacaggcg gcttccagtt tggcgccgtc
     2941 ggggtcgaga atgaaggcgc cgatataggt cgtcatggcc gcctggcgcg gtcccggcgc
     3001 gccggcgtcg gccccgccat gggccagcgc ggccgtgtgg aaggcctcca ccgcttcagg
     3061 actcgaggct cgcaggcaga cgtggtgacc ggtgtccggg tcggcccggg tcacccccgg
     3121 gcgatgattg agccacacct cggggtagcg cttgccgaac ccgacggtcc ccggccgatc
     3181 gaccagccgg cggtagccga gcggacccag cacggcgtca tagaaggcgg cgcttctgac
     3241 cagatcactc accgcgatgg agagatggtc gatcatggac ccttacagcg tcctggcgat
     3301 cagcaccttc atgatctcgt tcgtgccgcc gtagatccgc tgcacgcggc tgtcgcggta
     3361 catgcgggcg atcgggtatt cggccatgta gccgaagccg ccgaacagct gcaggcagcg
     3421 gtcgatgatc ttgttctcga gatcggtcag ccagtatttg gccatcgagg ccgtcgccgc
     3481 gtccagcttg cccttcaggt gggcctcgat gcagtggtcg acgaacaccc gcgccaccgt
     3541 cgcctcggtc ttgcattcgg ccagcacgaa ctgggtgttc tggaagtcga tgaccttctt
     3601 gccgaaggcc tcgcgctgct tgacgtagtc gatggtcagg gccagcgccc gctcgatggt
     3661 ggcgatggcc tggacggcga tgttcagccg ctcctgcggc agctggccca tcagctggaa
     3721 gaagccctgg ccctcgccgc cgcccagcag gttggcggcc gggacccgca cgtcgttgaa
     3781 gaacagctcg gacgtgtcgg ccgcctcctg gcccagcttg tcgaggttgc ggccgcgctc
     3841 gaaccccggc gcgttgtcgg tctcgaccac gatcagcgag gtgccgcgcg cgccctgggt
     3901 cgggtcggtc ttgcagacga cgatgatgaa gttggccgtc tggccgttgg tgatgaaggt
     3961 cttggacccg ttgatgacgt aggcgtcgcc atcgagcctg gccgtggtct tgatgccctg
     4021 caggtcgctg ccggcgccgg gctcggtcat ggcgatggcg ctgacatatt cgcagctcgc
     4081 cagcttcggc agccagcggc gcttctgctc ctcggtgccg caatgcagga tgtagggggc
     4141 gacaatggcg ttgtgcagcg acaggccgaa cccatcgacg cccttggcgc ccagctgctc
     4201 catgaggatg acctcatggc ggtagtcgcc gcccatgccg ccgtattcct ccgggatcga
     4261 caggcaggaa aggccggcct cggccgcctc gttccagaac agccgctcga ccttgccgtc
     4321 ctcgcgccac ttcgcgacgc gcgattcggg ggccttctcg tcgaagaacc ggccgacgga
     4381 gtcctcgaag atgacaatgt cctcctcggc cagggagggc gtgcgatcga ggttgagaac
     4441 gcccatcaga aggcctccgc gggaatggac atcatgttgg ccgagccggt cttcagcttg
     4501 gccagatgcg cgcccgcgtc aggcaggatg cgctcaagga aatactgacc gaccgagacc
     4561 ttgctggcat agaaagggtc ggtgtcgccg gccgcgatct tcgcgagcga caccttggcc
     4621 atcagggccc acatgtaggc caggccggtg acgccgaaga ggtgcatgta gtcggtcgag
     4681 gcggccccgg cgttgtcggg gttctgcagg ccgttctgca tcagccacat cgtgccgtcc
     4741 tgcagctggg ccttggccga ggccaggccg tcgaggaacg gcttgagctt ttcgtcgccg
     4801 gcgttttcct cgacgaagcc gtcgatctcg gcgaaccagc tcatcaccgc ccggccgccg
     4861 ttggccgcca gcttgcggcc gacaaggtcg agcgcctgga tgccgttggt gccctcgtag
     4921 atcagggcga tacggcagtc gcgcagatac tggctggccg ggaaatgctc ggtgaagccg
     4981 ctgccgccgt gcacctgcat gccgtcgacg caggccttga agcccttgtc ggtcaggtag
     5041 cccttcagca ccggcgtcag cagacccatg tagtcgccgg ccttctggcg cacggcctcg
     5101 tcggggtggc cgtggctcag gtcgccgtgc agcgcggtcc agaacagaaa ggcgcggccg
     5161 ccctcgatga ccgcacggct gtcgagcagc atgcggcgca cgtcggggtg gacgatgatc
     5221 ggatcggccg gaccctccgg attcttcggc ccggtcaggg aacggccctg cagccggtcc
     5281 ttggcgaagt cgcgcgcggc ctggtaggcg gcttcgccct gggccacgcc ctgcaggccc
     5341 acgccgatgc gggcctcgtt catcatcacg aacatcagct tcaggccgct gttctcttcg
     5401 ccgatcagcc agcccttggc gtcctcgtac tgcatcacgc aggtggcgtt gccgtggatg
     5461 cccatcttct cctcgaggcc gacgcacttg acgccctcgt tgcgcggacc gaccgacccg
     5521 tcggggttgg gcaggaactt cggcacgatg aacaggctga tgcccttgac gccggccggc
     5581 gcgccctcga cgcgggcgag caccaggtgg acaatgttgt cagccaggtc gtgttcgccg
     5641 gcgctgatcc agatcttctg gccactgatg cggtagctgc cgtcggcctc ggggaccgcc
     5701 ttggtgcgca acaggcccag gtccgtgccg caatgcggct cggtcaggtt catggtgccg
     5761 gtccattcac cggagaccat cttgggcaga taggtctgct tctgcgcgtc gctgccgccc
     5821 gtgtggatcg ccgaataggc cccgtgggcc aggcccggat acatcgagaa ggccatgttg
     5881 gccgagctcg acatctcgct gaacgacagg ttcaccacgt gcggcaggcc ctggccgccg
     5941 taggccggat cggaccccag tcccgtccag ccgccggcgc acagctgctg gtaggcctcc
     6001 ctgaacccgg ccggggtctt gacggtgaag tcgggactcc actggcagcc ctgcttgtcg
     6061 cccacggcgt tgagcggcgc cagcaccccg gcggtgaact tcgccgcctc ctcgatgatc
     6121 tgttcgacga catccatcga ggcgtcggcg aaggcgggca ggctggaata gcggtcgatc
     6181 tccagcacgt cgcgcaggag aaacacgtga tcgcgagccg gcggctgata ggccatgggc
     6241 gctccgttgg gtcggggttc tggttgttcg gtgtcggcct atttggcgcc gagcatttcc
     6301 ggatggccgt cgagacgggc gcgcagcatc tggtcgtagt cggcggcctt gggcagcagg
     6361 tccggccgtt tggcggccag gtgcttttcg aggcgttcgc aggcttcccg cagctcgttg
     6421 atggcgccct cgatgtcctc gcgctgctgc tcgagatcga cgatcttttc ccggaatttc
     6481 ttcagcgaat gggcgttctg ggtcatcgcc tcgtcgtgct cgccgtagag ctcgaggatc
     6541 tcccggatct cctgcagcga caggcccacg cgcttgccgc gcaggatcag gatcagccgc
     6601 gcgcgatcct tgtaggaata cacccggttc atcccgtcgc gcgcgggggt cagcaggccc
     6661 ttgtcctcat agaagcgcag cgcccgcggc gtggctttga actccgtgca cagctgccgg
     6721 atggtgaagg tacgctcggg gcgacgcagt tcggctggca ttttttcctc ccgtgccacg
     6781 gtttgcgttt tttcgagccg tatatgccct ttacgtcaac gtcaacaatc gcaattgtgt
     6841 gatgtttact ccagattgta tccttgcgcc ttctctcgct ctgtgattgt tcttccctcc
     6901 gcgcgggtgg accagactgg ggagggtcag atggacagct tgccggtggc gtcagccgtc
     6961 gccagggcgt tggtcacaac ggaatcggcg gtggatgagg cgctcgtccg ggcggtcgcg
     7021 ctgatgcggc ggatgatcag cggccgccgc gagctcggcc tgcccgtggc cttgacggac
     7081 ccggtgatgg gccgggtgtc ggccgctgtg aacgccctgg gcgacgccca tcgggaactg
     7141 gttcgcgcgc acggcgacct ggcgcgcatc ggcgcggaac tcggtctggg cgacctcggc
     7201 ttcggcccgc tggtcaagcc gaccgggggg ccggcggagc cccagccggt cgactgagcc
     7261 gtggccctgc tgttgttcgc cctcctgctg gcgatcgccg cgaccggcgc cttggtcagg
     7321 ggtggactga cggaacggcg gggcgcggcg ctgttcacgt cggcctggat cgtgtcgctc
     7381 gccgcgcagt gggtttccgg gcgggccgcc cctggcctgt ggctgccggt gatcgacgcg
     7441 gcggtgctgg tcgggctcgc ggccctcgcc tggcgctcac cgaggccctg gcccgtctat
     7501 gccagcggct ttcagctgtt gaccctgacc ggcgacatcg cccgctgggt ggagaccgac
     7561 ctcgatgtcc ggatccatct gaccctgct
//
LOCUS       DAORNX010000095         7466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7314_length_7466_cov_7.517474, whole genome shotgun sequence.
ACCESSION   DAORNX010000095 DAORNX010000000
VERSION     DAORNX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7314_length_7466_cov_7.517474"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..721
                     /locus_tag="PLO65_05820"
     CDS             266..721
                     /locus_tag="PLO65_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HRD27799.1"
                     /translation="MHRRVFAIAMFSAFALPVGAQERPPPERQTLLDLAYALGESHAL
                     RQACEGESDQFWRDRMVRLTDTEAADAAFDGRLKQQFNSGFATRQTEFPSCSPASKRA
                     EQAVARKGQALAGRLSSITRVVRDLGSDAPVEESEAAGPDSVAAPPAPR"
     gene            867..1826
                     /locus_tag="PLO65_05825"
     CDS             867..1826
                     /locus_tag="PLO65_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD27800.1"
                     /translation="MSFAIFAVVLIVLALFLLVSVIKIVPQGREYTVERFGRYTRTLK
                     PGIAILTPFIEGIGRRVNMMEQVLDVPQQEVITKDNAVVKVDGIVFIQVMDAAAAAYR
                     VDNLTYAISQLCMTNLRTVVGSMELDEVLSQRDQINTRLLATIDHATNPWGVKATRIE
                     IKDLTPPPDITNAMARQMKAERERRAVITEADGEKQAAIARAEGAKQSAILQAEGRRE
                     AAFRDAEAREREAEAEAKATAFVSEAIARGDLNAINYFVAQKYVEAFASLAASPNSKM
                     IVVPADFAGITGAVAGIGELVKSLADNRPSSGGGGAAVPRTKA"
     gene            1837..2295
                     /locus_tag="PLO65_05830"
     CDS             1837..2295
                     /locus_tag="PLO65_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRD27801.1"
                     /translation="MTWLADLYVAHPFWIWAAIAALILAAEVATGTGWLLWPAACAAV
                     VAALTVLLKGDFTVELAVFAVLTIASTLLARRYLPRNASGEGPDINDNVGRLVGHVGT
                     AVGAFEHGRGRVFIDGKEWAAVTDEDAPPAADQAVKVIATDGGILKVKPH"
     gene            complement(2311..2757)
                     /locus_tag="PLO65_05835"
     CDS             complement(2311..2757)
                     /locus_tag="PLO65_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD27802.1"
                     /translation="MAAEPRDRPDVRVMTEIGIIAQLSTASLERTLPDGMSAAQFGVL
                     THFMRRGGTETPAQLARAFQVTKGAMTNTLQRLEAQGFVRIVGDETDGRRKLVTLTEA
                     GARAFNAGLGAMKPHLDAMRAAFTEDEFKAALPFLQALRIWLDENR"
     gene            complement(2757..3893)
                     /locus_tag="PLO65_05840"
     CDS             complement(2757..3893)
                     /locus_tag="PLO65_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27803.1"
                     /translation="MRRRDFIRILGGGAILAATACAPTESDPRAAWVNPGAGETDLRR
                     KALSWAILAPNPHNMQPWIVDLSVADEITLYIDKTRLLPVTDPFNRQITIGCGAFLEL
                     LVMALAEFGGVSATTDLFPRGEAHPLLDDRPLFRVRLASRGATRSPLFSQILNRRTNR
                     GPFTEREVDPSAAANLLGGFQPVGGADCTVTVTPERVARLRALVYRGAEIEAHTPSAH
                     RESVERTFIGARAVAEHPWGISLDTPMMTAMYSVGLLTQAKMATPGSIAFREALKFLK
                     TAADTSQSFVWITTATNTRAEQVAAGRAYVLANLSAAKFGLAMHPWSQGLQEYATQKP
                     VFDALHRELAPTGGRIQMLARIGYPKDDVPPAPRRGLAAQLRKT"
     gene            complement(3949..4395)
                     /gene="rnhA"
                     /locus_tag="PLO65_05845"
     CDS             complement(3949..4395)
                     /gene="rnhA"
                     /locus_tag="PLO65_05845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRD27804.1"
                     /translation="MTPKVLIYTDGACRGNPGPGGWGAILISGGREKELCGGERATTN
                     NRMELMAAIQALEALNKPCKVELHTDSQYMRNGIMTWIHGWKARGWKTADKSPVKNED
                     LWKRLDAARARHEVDWRWVKGHAGHDLNERADGLANRGMKEMLREA"
     gene            complement(4392..5351)
                     /gene="thrB"
                     /locus_tag="PLO65_05850"
     CDS             complement(4392..5351)
                     /gene="thrB"
                     /locus_tag="PLO65_05850"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRD27805.1"
                     /translation="MAVYTDISDDELAALLAEFDIGQAIAFKGIAEGVENSNFLLETD
                     RGRYILTLYERRVRADDLPYFLNLLTWLADHGFPSARPVPDRQGRTLKTVRDKPAAIV
                     EFLIGISVRRPTAAHCREAGAGLARLHLAGQGYAGRRENDLGQPAWAPMFAGLWAEAE
                     ALKPGLAETIRADLDYLGRDWPRGLPTGVVHADFFPDNVFFRDGRFAAAFDFYFACDD
                     AYAYDLAIALNAWCFEPDGSFNLTAGRAMLAGYQAHRPLGVAERAALPILAHGAAMRF
                     FLTRLNDWGATPAGALVRPKDPLEYERKLAVHRAGLALLGDHA"
     gene            complement(5508..6467)
                     /gene="ispH"
                     /locus_tag="PLO65_05855"
                     /gene_synonym="lytB"
     CDS             complement(5508..6467)
                     /gene="ispH"
                     /locus_tag="PLO65_05855"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRD27806.1"
                     /translation="MDDAAPTKPPLKVLLASPRGFCAGVDRAIQIVERAIEKYGAPVY
                     VRHEIVHNRHVVERLKALGAVFIEELDEAPTDRPVVFSAHGVPKSVPAEARSRKMLFL
                     DATCPLVSKVHVEAQKHYDAGREIVLIGHKGHPEVVGTMGQLPEGAVRLIEHVADAEV
                     FEPADAANVAFVTQTTLSVDDTADIVAVLQRRFPGIAAPHKEDICYATTNRQDAVKLL
                     ARDSDLLLVVGSRNSSNSVRLVEVGLRGGARNARLIDDAVGLDWAWLDGVATVGVTAG
                     ASAPEVLVQGVIDKLAGRYDLTVEDVAPTRETVTFKLPRVLTT"
     gene            6607..7308
                     /locus_tag="PLO65_05860"
     CDS             6607..7308
                     /locus_tag="PLO65_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HRD27807.1"
                     /translation="MRRTVNIGLLALFAAGSLAVASPVLAQGRPGGADDQRRQEEDAA
                     RKKKRDQEWNQPAAPLPQLRNAGPCPFVKVLYDAGRYIEFKDGKESAATVGWTGEIQG
                     VSAGCEYKDADPIKVKVELLFAMGRGPVAVGESKTYRYWVAVTQRNQAVIAKEYFDLP
                     VNFSAGEDRVFVRDSIANITIPRANADTSGANFEVLIGFDVTQQMAAFNRDGKRFRVN
                     AGAAAATPAGAPTTP"
     gene            7305..>7466
                     /locus_tag="PLO65_05865"
     CDS             7305..>7466
                     /locus_tag="PLO65_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27808.1"
                     /translation="MSDLKRSLGEAVAAAFVAQGLPAELGRVTASDRPDLADFQCNGA
                     LPAAKVAGRP"
BASE COUNT         1137 a         2547 c         2656 g         1126 t
ORIGIN      
        1 gcgccgccgt ctcgatggcg gcgtgaaggg ccagcgccag cgccagggcg acggccagga
       61 ccagcgcgct cgcgacccag ggcggaaggg cgcgggcgcg gtcggccagc tcggtgaaca
      121 ggaattccgg actcacggcc gggacggctt ggggacgaac gccatggtcg ggaaacagat
      181 tcccccgccc ggagttccgc ccgatcacct cgcccgtggc ttgcgcaccg gtgacagcgg
      241 ggcccgcttg ccgttacatc gggccatgca caggcgcgtc ttcgccatcg ccatgttcag
      301 cgccttcgcg ctgcccgttg gagcccagga gcggccgccg cccgagcggc agaccctgct
      361 cgacctcgcc tatgcgctgg gcgagagcca cgcgctgaga caggcctgtg aaggcgagag
      421 cgatcagttc tggcgcgacc gcatggtccg gctgaccgac accgaagccg ccgacgcggc
      481 cttcgacggc cggctgaaac agcagttcaa cagtggcttc gccacccgtc agacggagtt
      541 cccgtcctgc agcccggcca gcaagcgggc cgagcaggcc gtggcgcgca agggccaggc
      601 gctggccggc cggctcagca gcatcacccg cgtggtgcgc gatctgggct ccgacgcccc
      661 ggtcgaagaa tcggaagcgg ccggtccgga ttccgtggcg gcgccccccg cgccgcgcta
      721 gattcgcctc caggagcctt tctcgcgcag gtcatttcga tccgcgcgag agaaaaggct
      781 ccttgatttc tgcttgccgc gcttcctgat cgccaaaccg gcaaccacct tggcgggaag
      841 cgcggcgagt agtctggagg ggacaaatgt ccttcgcaat tttcgccgtc gtgctgatcg
      901 tgctggcgct gttcctgctg gtcagcgtga tcaagatcgt gccccagggg cgcgagtaca
      961 ccgtcgagcg gttcggccgc tacacccgca cgctcaagcc ggggatcgcc atcctgacgc
     1021 ccttcatcga ggggatcggc cgccgcgtga acatgatgga gcaggtgctc gacgttccgc
     1081 agcaggaggt catcaccaag gacaacgccg tggtgaaggt cgacggcatc gtcttcatcc
     1141 aggtgatgga cgcggccgcg gcggcctatc gcgtggacaa cctgacctat gccatcagcc
     1201 agctgtgcat gaccaacctg cgcacggtgg ttggctcgat ggagctggac gaggtgctca
     1261 gccagcgcga ccagatcaac acccgcctgc tggcgaccat cgaccacgcg accaacccct
     1321 ggggcgtcaa ggccacgcgc atcgagatca aggacctgac cccgccgccg gacatcacca
     1381 acgccatggc gcgccagatg aaggccgagc gcgaacggcg cgcggtgatc accgaggccg
     1441 atggcgagaa gcaggcggcc atcgcccgcg ccgaaggggc caagcagtcg gccatcctgc
     1501 aggccgaggg ccggcgcgag gccgcgttcc gcgacgccga ggcgcgcgag cgggaagccg
     1561 aggccgaggc caaggccacg gccttcgtgt ccgaggccat cgcccgcggc gacctcaacg
     1621 cgatcaacta cttcgtggcg cagaagtacg tcgaggcctt cgccagcctc gcggcgagcc
     1681 ccaactcgaa gatgatcgtc gtgccggccg acttcgccgg catcaccggc gccgtggccg
     1741 ggattggcga gctggtgaag tcgctggccg acaaccggcc gtcgtcgggc gggggcggcg
     1801 cggccgttcc ccgcaccaag gcctaggggg accgccatga cctggctggc tgacctctat
     1861 gtcgcccatc cgttctggat ctgggcggcg atcgcggcgc tgatcctggc ggccgaggtg
     1921 gccaccggca ccggctggct gctgtggccg gccgcctgcg cggccgtggt ggccgcgctg
     1981 actgtcctgc tgaagggcga ctttacggtc gagctggcgg ttttcgcggt gctgaccatc
     2041 gcctcgacgc tgctggcccg gcgctacctg ccgcggaacg cctcgggcga ggggccggac
     2101 atcaatgaca acgtcggccg gctggtcggg catgtcggca cggcggtcgg ggccttcgag
     2161 cacggccgcg gccgggtgtt catcgacggc aaggaatggg ccgcggtgac cgacgaggac
     2221 gcgcccccgg cggccgacca ggccgtcaag gtgatcgcga ccgatggcgg catcctgaag
     2281 gtgaagccgc actgaggcgt tcgctggacg ctaccggttc tcgtccagcc atatccgcag
     2341 cgcctgcagg aacggcaagg cggccttgaa ctcgtcctcg gtgaaggccg cgcgcatggc
     2401 gtccagatgc ggcttcatcg cgccgagtcc ggcgttgaac gcccgcgccc cggcctccgt
     2461 gagggtgacc agcttgcgcc ggccgtcggt ctcgtcgccg acgatcctga cgaagccctg
     2521 ggcctcgagt cgctgcaggg tgttggtcat cgcccccttg gtgacctgga acgcccgggc
     2581 cagttgggcc ggcgtctcgg tcccgccgcg acgcatgaag tgggtcaaca cgccgaactg
     2641 cgccgcgctc atgccgtccg gcagggtgcg ctcgagactg gccgtcgaca gctgggcgat
     2701 gatgccgatc tcggtcatca cccgcacatc ggggcgatcc ctgggttcgg cggccatcag
     2761 gtcttcctca gctgggcggc caggccccgg cgcggcgcgg gcgggacatc gtccttcggg
     2821 tagccgatcc gggccagcat ctggatgcgg ccgccggtcg gagccagctc cctgtgcagg
     2881 gcgtcgaaga ccggcttctg ggtggcgtat tcctgcaggc cctgactcca gggatgcatg
     2941 gccaggccga acttcgcggc ggacaggttg gccaggacgt aggcccgtcc ggcggcgacc
     3001 tgctcggcgc gggtgttggt ggcggtcgtg atccagacga agctctggct ggtgtcggcg
     3061 gcggtcttca ggaacttcag cgcttccctg aaggcgatcg agcccggcgt ggccatcttc
     3121 gcctgggtca gcaggccgac gctgtacatg gccgtcatca tcggggtgtc gagcgagatg
     3181 ccccacggat gttcggcgac cgccctggcg ccgatgaagg tccgctcgac gctctcgcga
     3241 tgggctgacg gggtatgggc ctcgatctcg gcgccccggt agaccagcgc gcgcaggcgg
     3301 gccacgcgtt cgggagtcac ggtgaccgtg cagtcggcgc cgccgaccgg ctgaaagccg
     3361 ccaagcaggt tggccgccgc cgatggatcg acctctcgct cggtgaacgg cccgcggttg
     3421 gttcggcggt tgaggatctg gctgaacagc ggcgaccggg tcgcgccccg cgaggcgagc
     3481 ctgacgcgga acagaggccg gtcgtcgagg agcggatgcg cctcgccccg gggaaacagg
     3541 tctgttgtcg ccgagacgcc gccaaactcc gccagcgcca tgacgagcaa ctcgaggaac
     3601 gcgccgcagc cgatggtgat ctgtcgattg aacgggtcgg tgaccggcag caggcgggtc
     3661 ttgtcgatgt agagcgtgat ctcgtcggcg accgacaggt cgacgatcca cggctgcatg
     3721 ttgtgcgggt tgggcgccag gatcgcccag gacagggcct tgcggcgcag gtcggtctcg
     3781 ccggcgcccg gattgaccca ggccgcgcgg gggtcgctct ccgtgggagc gcaggcggtg
     3841 gcggccagga tcgccccgcc tccgagaatt cggatgaagt cacgtcggcg catggcgaag
     3901 cccttaattc aacgtagaac cattcataat ggtttaatat cgaaccagtc aagcctcgcg
     3961 gagcatctcc ttcatgcccc ggttggccag gccgtcggcg cgctcgttca ggtcatggcc
     4021 ggcgtggccc ttgacccagc gccagtcgac ctcgtgtcgc gcccgggccg cgtcgagccg
     4081 cttccacagg tcctcgttct tcaccggact tttgtcggcc gtcttccagc cgcgcgcctt
     4141 ccagccgtgg atccaggtca tgatgccgtt gcgcatgtac tggctgtcag tgtgcagctc
     4201 gaccttgcag ggcttgttca gcgcctccag cgcctggatg gcggccatca gttccatgcg
     4261 gttgttggtc gtggcgcgct caccgccgca caactccttc tcccggccgc ccgagatcag
     4321 gatcgcgccc cagccgccgg gcccgggatt cccgcggcag gcgccgtccg tgtagatcag
     4381 caccttcggc gtcatgcgtg gtcgccgaga agggccaggc cggcgcggtg caccgcgagc
     4441 ttgcgctcgt actcgagcgg gtccttgggc cgcaccaggg cgccggccgg ggtcgcgccc
     4501 cagtcgttga ggcgagtgag gaaaaagcgc atggccgcgc cgtgagcgag gatcggcagg
     4561 gccgcccgct ccgcgacgcc gagcggccga tgcgcctggt agccggccag catggcgcga
     4621 ccggcggtca gattgaacga gccatcgggc tcgaagcacc aggcgttcag ggcgatggcc
     4681 aggtcgtagg cgtaggcatc gtcgcaggcg aagtagaagt cgaaggcggc ggcgaacctg
     4741 ccgtcgcgaa agaacacatt gtccgggaag aagtcggcgt ggacgacgcc ggtcggcagg
     4801 ccccgcggcc agtcgcggcc cagatagtcg aggtcggcgc ggatggtttc ggccaggccc
     4861 ggcttgagcg cctcggcctc ggcccagagg ccggcgaaca tgggcgccca ggccggctgg
     4921 cccaggtcat tctcgcgccg cccggcatag ccctggccgg ccaggtggag ccgcgccaag
     4981 ccggccccgg cctcgcggca gtgggcggcc gtcggccggc ggaccgagat gccgatcagg
     5041 aattcgacga tggccgccgg cttgtcgcgc accgtcttca gggtccggcc ctgccggtcc
     5101 ggcaccggcc gcgcgctggg gaagccgtgg tcggccagcc aggtcagcag gttgaggaaa
     5161 tagggcaggt cgtccgcgcg cacccgccgc tcgtaaaggg tcaggatgta gcggccgcga
     5221 tcggtctcga gcaggaagtt ggagttctcc accccctcgg cgatgccctt gaaggcgatc
     5281 gcctgaccga tgtcgaattc ggccagcagc gccgccagtt cgtcgtcgga gatgtcggtg
     5341 tagacggcca tgcgaggggg atgggcgacg gcgatcggca cgtcaagttg ggagacgccg
     5401 ggtggcgggc cggatgcgca atgcggatgc cgcggccggt cacggcgaag accggctacc
     5461 gggggtgacc cggcggcggt cccgaccggc cggccgcgcg ggatcgccta ggtcgtcagc
     5521 acacgcggca gcttgaaggt cacggtctcc cgcgtcggcg ccacgtcctc gaccgtcagg
     5581 tcgtagcggc cggccagctt gtcgatcacc ccctggacca gcacctcggg ggccgaggcg
     5641 ccggcggtga cgccgaccgt cgcgacgccg tcgagccagg cccagtcgag tccgacggcg
     5701 tcatcgatca gccgggcgtt cctcgccccg ccgcgcagcc cgacctcgac cagacgcacg
     5761 gaattggacg agttgcgcga ccccacgacc agcaacaggt cactgtcccg ggcgagcagc
     5821 ttcacggcgt cctggcggtt ggtggtggcg tagcagatgt cttccttgtg cggcgcggcg
     5881 atgccgggga agcgccgctg cagcaccgcg acgatgtcgg cggtgtcgtc gaccgacagg
     5941 gtggtctggg tgacgaaggc cacattggcg gcgtcggccg gctcgaagac ctccgcgtcg
     6001 gcgacgtgct cgatcaggcg caccgcgccc tcgggcagct gacccatggt gccgaccacc
     6061 tcgggatgac ccttgtgacc gatcagcacg atttcgcggc cggcgtcgta gtgcttctgc
     6121 gcctccacat ggaccttgga caccagcggg caggtggcgt cgagaaacag catcttgcgc
     6181 gaccgggcct cggccgggac agatttgggc acgccatggg ctgaaaacac caccggccga
     6241 tcggtcggcg cctcgtccag ctcctcgatg aagacggcgc ccagggcctt cagccgctcg
     6301 accacgtgtc tgttgtgcac gatctcgtgc cgcacataga ccggcgcgcc gtatttctcg
     6361 atcgcccgct cgacgatctg gatcgcccgg tcgacgccgg cgcagaagcc gcgcggactg
     6421 gccagcagga ccttcagggg cggctttgtc ggggcggcgt catccatggc ggcaaggtag
     6481 gcggtcgtgg tcactcccgc cagcggtgaa cccgcgttgc gaggccacgc atatgccttt
     6541 atcagactgc atctgctttt cggccggcac gcccgcgccg gttccctgct gttttccgga
     6601 cgtgacatgc gccgcactgt gaatattgga cttctcgccc tgttcgccgc cggctcgctg
     6661 gcggtcgcca gtccggtgct ggcccagggg cgtcccggcg gcgccgacga ccagcgccgg
     6721 caggaggagg acgcggcgcg caagaagaag cgggaccagg agtggaacca gccggccgca
     6781 ccgctgccgc agctgcgcaa cgccggaccc tgtcccttcg tgaaggtgct ttacgacgcc
     6841 gggcgctaca tcgaattcaa ggacggcaag gaaagcgccg ccaccgtcgg ctggaccggc
     6901 gagatccagg gcgtctcggc gggctgcgag tacaaggacg ccgacccgat caaggtcaag
     6961 gtcgagctgt tgttcgctat gggccggggc cctgtcgccg tgggcgagtc caagacctat
     7021 cgctactggg tcgccgtgac ccagcgcaac caggcggtca tcgccaagga atacttcgat
     7081 ctgccggtga acttctcggc cggcgaggac cgggtgttcg tgcgcgacag catcgccaac
     7141 atcaccattc cccgggccaa cgccgacacc agcggggcca acttcgaggt cctgatcggg
     7201 ttcgacgtga cgcagcagat ggcggccttc aaccgtgacg gcaagcgctt ccgggtcaac
     7261 gccggggccg ccgcggcgac gccggccgga gcccccacca ccccatgagc gacctgaaac
     7321 ggtccctcgg cgaagcggtc gctgccgcct tcgtcgccca gggacttcct gctgaactgg
     7381 gccgggtgac cgcatcggat cgcccggacc tggccgactt ccagtgcaac ggcgccctgc
     7441 cggcggccaa ggtcgcgggc cgcccg
//
LOCUS       DAORNX010000096         7462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7322_length_7462_cov_6.803159, whole genome shotgun sequence.
ACCESSION   DAORNX010000096 DAORNX010000000
VERSION     DAORNX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7322_length_7462_cov_6.803159"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>322)
                     /locus_tag="PLO65_05870"
     CDS             complement(<1..>322)
                     /locus_tag="PLO65_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRD27809.1"
                     /translation="AGVSGRQFFLDRGLGETRGVVTLDGRPERLLIQRDGDSEVAALG
                     ARSVARVRSVDKAIGMAFLDLPGGVEAIHSLRTDEPPPVRGAAVEVEIRTEPRQDKLA
                     KVRLI"
     assembly_gap    323..426
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<427..892)
                     /locus_tag="PLO65_05875"
     CDS             complement(<427..892)
                     /locus_tag="PLO65_05875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HRD27810.1"
                     /translation="MSGTKSPLVLASASPRRLELLRQVGIEPDHVDPADIDETPRRDE
                     TPRRLALRLAREKASAVASRHPHAFVVGADTLVAVGRRILNKPGTEAEARAMLALLSG
                     RAHKVLTGLSIVAPGGRSAERVVETRVRFKRLSDLETNAFIAAGDWRDAAGGY"
     gene            complement(889..1107)
                     /gene="infA"
                     /locus_tag="PLO65_05880"
     CDS             complement(889..1107)
                     /gene="infA"
                     /locus_tag="PLO65_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRD27811.1"
                     /translation="MAKEELLEFPGTVSELLPNATFRVKLENDHEIIAHTAGKMRKNR
                     IRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            complement(1199..1654)
                     /locus_tag="PLO65_05885"
     CDS             complement(1199..1654)
                     /locus_tag="PLO65_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRD27812.1"
                     /translation="MEGLPGAVLFACNFNRVRSPMAEALMRMQFGDRVFVDSVGLRRE
                     PGETAVDPFAAAVIDEIGGDLTDFRPKTFDDLEDESFDLVVSLTPEAQHRAVELARGR
                     AVDIEYWPTLDPTLADGTREQRLEAYRQVRDELARRIRARFGKASTFGG"
     gene            complement(1661..2137)
                     /locus_tag="PLO65_05890"
     CDS             complement(1661..2137)
                     /locus_tag="PLO65_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HRD27813.1"
                     /translation="MTDDRAIHRLRTIELDEDSLGAASRDQEQERQIAIFDLLEDNFF
                     RPEGATGGPYDLKIALVENRLALHITGPDLDVTHLLSLSPFRGVIRDYFMICESYYQA
                     IRNATPAQIEALDMGRRGLHNEGSTLLQARLEGKVKTDLDTARRLFTLICALHWRG"
     gene            complement(2160..3446)
                     /gene="hisD"
                     /locus_tag="PLO65_05895"
     CDS             complement(2160..3446)
                     /gene="hisD"
                     /locus_tag="PLO65_05895"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRD27814.1"
                     /translation="MRRFRSTDPDFTAAFRSFLNERRGSPADVDAAAAEVLENVKARG
                     LEAVLDYTRKFDRVDLTEATVRVTAEEIEAGAAACDPAVREAIAFAAARIRAYHTRQK
                     PADQQWTDEAGVELGWRWTPLEAVGVYVPGGRAAYPSTVLMNCVPAAVAGVDRIAMVT
                     PPGKLQPAVLAAAKEAGVTEIWRIGGAQAVAALAYGAGPVLPVDKIVGPGNAWVTAAK
                     RRLYGVVGIDALAGPSEIVVVADADNDPDWIAADLLSQAEHDPAAQSILITDDAAFAA
                     AVEAAVTRGLTTLATGEDAAASWRDHGAVIIAPLGESPALVDLIAPEHVEFCVAAPES
                     LADRVRHAGAIFLGRHTPEAIGDYVAGSNHVLPTSRAARFQSGLSVYDFLKRTSIVRC
                     DAAAFARLGPHTVALARAEGLPAHALSAALRLPSGD"
     gene            complement(3486..3839)
                     /locus_tag="PLO65_05900"
     CDS             complement(3486..3839)
                     /locus_tag="PLO65_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27815.1"
                     /translation="MRRSIPALTALLLAAVVSGAASAEQTDADLCRLERSTPRDVVDF
                     MQRWADCAHWGGEEAYDADRDRQIRRAVRELRCDRLDRDEAALRRKYAGRTAVLAALD
                     IGKDQLAWPECPRRQ"
     gene            complement(3852..4283)
                     /locus_tag="PLO65_05905"
     CDS             complement(3852..4283)
                     /locus_tag="PLO65_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HRD27816.1"
                     /translation="MASPAPLRLLAQDAEDLAVISAALQDAVAKIGDIAFEPRARRLT
                     VAFNRYRWERDGRTHERVRSGLQLGGVMAVRSRNLRRDSKGAVVELLALNFEPGEAPG
                     GAVVFTFAGGGDLRAEVECLEAVLADVSTPWPTPRTPTHGD"
     gene            complement(4286..5581)
                     /gene="murA"
                     /locus_tag="PLO65_05910"
     CDS             complement(4286..5581)
                     /gene="murA"
                     /locus_tag="PLO65_05910"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRD27817.1"
                     /translation="MDRIAIVGGARLHGEIPISGAKNSAIKLMTASLLTEEPLHLTNM
                     PRLADTRFLGTLLTRLGTMVDEREGPAGSETLLHTPEIVSSFAPYDLVRQMRASFNVL
                     GPLIARTGQAKVSLPGGCTIGARPVDLHLKALEALGAQIDLHEGYVYAQAPRGLKGAE
                     IEFPFVSVGATEHAMLTAVLASGETVLANAACEPEIVDLADCLNKMGARVEGAGTPTI
                     RITGVRKLGGATHAVITDRIEAGTYAVAAAMAGGEVRLTNTRADLIEALLLKIEEAGA
                     GVERTNDGVIIRRNGARLKAVDIDTGVYPGFATDLQAQFMALMTLAEGESVIREHIFE
                     NRFMHAPELARLGADITVSGGEARVRGVEYLEGAQVMATDLRASVSLVIAGLAARGET
                     VVGRVYHLDRGFERLEEKLGACGADVRRIKGDPAPEAED"
     gene            complement(5678..6052)
                     /locus_tag="PLO65_05915"
     CDS             complement(5678..6052)
                     /locus_tag="PLO65_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27818.1"
                     /translation="MTHQANCARLAPRPYREVRHLRAARPPQGEPFDVTVEISDVFRL
                     EDSRDWACAMRIVGLDPEHRAEHGRAFFGVDPMQAIEHALFIAHVELELATEKLGLAF
                     ADGGPYVPVTLFRRPGEAGHQA"
     gene            complement(6049..6183)
                     /locus_tag="PLO65_05920"
     CDS             complement(6049..6183)
                     /locus_tag="PLO65_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27819.1"
                     /translation="MKNPPQAPPPEKADREAKLAQALRANLRRRKAAPRDGARPKGDE
                     "
     gene            6278..6352
                     /locus_tag="PLO65_05925"
     tRNA            6278..6352
                     /locus_tag="PLO65_05925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6310..6312,aa:Thr,seq:ggt)
     gene            6378..6971
                     /locus_tag="PLO65_05930"
     CDS             6378..6971
                     /locus_tag="PLO65_05930"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD27820.1"
                     /translation="MTRPRKDGGFPPADMPARTVHSGWLDLHLVELTNPDGSTYDRYV
                     EDHGRAAAVLPYNPTTRMALLVTMPRAALRFAGGGDVLEAPAGLIDPGETAADCARRE
                     LVEEAGLEAGTLEPAGAIIPSPGISTEQIDLFLAPYQDSQRTGAGGGLDGENENITVV
                     ETPLDRLWALIDGDALSDAKTRVLVQTLRLRRPELFS"
     gene            complement(6964..7362)
                     /locus_tag="PLO65_05935"
     CDS             complement(6964..7362)
                     /locus_tag="PLO65_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRD27821.1"
                     /translation="MTWIAAYVAAAVSFLGLDALWLGLIARTLYQREFGALLLEKPNM
                     AAAAAFYALYLGGVVFFAVKPALDGGGWTRALLNGALFGLVAYATYDLTNLATLKGFP
                     IRVVAPDLAWGAAVTAIAALAGYAAAARVS"
BASE COUNT         1076 a         2598 c         2582 g         1102 t
ORIGIN      
        1 cgatcagccg caccttcgcc agcttgtcct gacgcggctc ggtgcgaatc tcgacctcga
       61 cggccgcgcc gcgcacgggt ggcggttcgt cggtccgcag ggaatggatc gcctcgacgc
      121 cgcccggcag gtcgaggaag gccatgccga tcgccttgtc cacgctgcgc acccgggcca
      181 cgctgcgggc gccgagggcg gccacctcgc tgtcgccgtc ccgctggatc agcaaccgtt
      241 cgggacgccc gtcgagcgtg accacgccgc gggtctcgcc caggccccga tcgagaaaga
      301 actgccggcc gctcacgccc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnntgta gccgccggcc gcgtcccgcc agtcgccggc ggcgatgaag gcgttcgtct
      481 ccagatcgct caggcgcttg aaccggacgc gcgtctcgac cacccgctcg gccgagcggc
      541 cgcccggcgc cacgatggac aggccggtca gcaccttgtg ggcgcggccc gacagcaggg
      601 ccagcatggc gcgggcctcg gcctcggttc cgggcttgtt gagaatccgc cggccgacgg
      661 cgaccagggt gtcggcgccg accacgaagg cgtgcggatg ccgcgaggcg acggcggacg
      721 ccttctcgcg ggcaaggcgc agggccagcc ggcgcggtgt ctcgtcgcgc cggggagttt
      781 cgtcgatgtc ggcggggtca acgtggtcgg gctcgatgcc gacctgcctg agaagctcaa
      841 gccgtctggg gctggcgctg gccaggacca gagggctttt cgtcccgctc acttgaagcg
      901 gtaggtgatg cgccccttgg tcaggtcgta gggcgtcatt tcgaccagca ccttgtcgcc
      961 ggccagaacg cggatgcggt tcttgcgcat cttgccggcg gtgtgggcga tgatctcgtg
     1021 atcgttttcc agtttcacac ggaacgtggc gttgggcagc agctcgctga ccgtgccggg
     1081 aaactccaga agttcttctt tagccatagg cctctcggat ggatcaaata agcaaacggc
     1141 ccgcgccgaa ctcgattcgg gtcgcgagcc gcggctccag accgccagta tagcgcgcct
     1201 atcccccgaa cgtcgatgcc ttgccgaaac gggcgcggat gcgacgggcc agctcgtccc
     1261 gcacctggcg ataggcctcc agccgctgct cacgcgtgcc gtccgccagc gtggggtcga
     1321 gggtcggcca gtattcgata tcgaccgccc tgccccgggc cagctcgacc gcgcgatgct
     1381 gcgcctcggg cgtcagggag accaccaggt cgaacgactc gtcctccagg tcatcgaaag
     1441 tcttgggtcg gaaatcggtc agatcgccgc cgatttcgtc aatcaccgcg gcggcgaacg
     1501 gatcgaccgc cgtctcgccc ggctcgcggc gcaggccgac gctgtcgacg aacacccggt
     1561 cgccgaactg catccgcatc agcgcctcgg ccatcggcga ccgcacgcga ttgaagttgc
     1621 aggcgaacag gaccgccccc ggaagcccct ccaccgccgg tcagccccgc cagtgcaggg
     1681 cgcagatcag ggtgaacagg cgccgggccg tgtccaggtc ggtcttcacc tttccctcga
     1741 gcctcgcctg cagcagcgtc gagccttcgt tgtgcaggcc gcgccggccc atatcgaggg
     1801 cctcgatctg cgccggcgtc gcgttgcgga tcgcctggta gtagctctcg cagatcatga
     1861 agtagtcgcg gatcacgccg cggaacggag acagcgacag caggtgcgtc acgtcgaggt
     1921 cgggaccggt gatgtgaagc gccaggcggt tttcgaccag ggcgatcttc aggtcatacg
     1981 gcccgccagt ggcgccctcc ggccggaaga agttgtcctc gagcaggtcg aagatcgcga
     2041 tctgacgttc ctgctcctga tcgcgcgagg ccgcgccgag ggaatcctcg tcgagctcga
     2101 tcgtccgtag acggtggatg gcgcggtcgt cggtcatgcg ggacggacag gttccccgtt
     2161 caatcgccgg aaggcaaccg cagcgcggcc gaaagcgcgt gggcgggcag gccctcggcc
     2221 cgcgcgaggg ccaccgtatg cgggcccagg cgggcgaagg cggcggcgtc gcaccgcacg
     2281 atcgaggtgc gcttgaggaa gtcatagacc gacaggcccg actggaagcg cgccgcgcgg
     2341 ctggtcggca gcacgtgatt ggacccggcc acatagtcgc cgatggcttc gggcgtgtgg
     2401 cggcccagga agatggcccc cgcgtgccgc acccggtcgg ccagggattc gggagcggcg
     2461 acgcagaact ccacgtgctc gggcgcgatc aggtcgacca gcgccgggct ctcgcccagc
     2521 ggggcgatga tcaccgcccc gtggtcgcgc caggaggcgg cggcgtcttc cccggtggcc
     2581 agggtcgtca ggccgcgcgt gacggcggcc tcgacggcgg cggcgaaggc cgcgtcatcg
     2641 gtgatcagga tcgactgggc ggccgggtcg tgctcggcct gcgacagcag atcggcggcg
     2701 atccagtcgg gatcgttgtc agcatcggcg acgacgacga tctcggacgg tccggcaagc
     2761 gcgtcgatgc cgaccacgcc gtacagccgg cgcttggcgg cggtgaccca ggcgttgccc
     2821 ggtccgacga tcttgtcgac cggcaggacg ggccctgccc cgtaggccag ggcggcgacc
     2881 gcctgggcgc cgccgatgcg ccagatctcg gtcacgcccg cctccttcgc cgcggccagc
     2941 accgctggct gcagctttcc cggcggggtc accatggcga tgcggtcgac gcccgcgaca
     3001 gcggcgggca cgcagttcat cagcaccgtc gagggatagg ccgcgcggcc gccggggaca
     3061 tagacgccaa ccgcctccag cggcgtccag cgccagccca gttcgactcc cgcctcgtcg
     3121 gtccactgct ggtcggcggg cttctggcgg gtgtggtaag cgcggatgcg cgcggcggcg
     3181 aaggcgatgg cttcgcgcac ggccgggtcg caggcggccg ccccggcctc gatctcttca
     3241 gcggtgaccc ggaccgtcgc ctcggtcagg tccacccggt cgaacttgcg ggtgtagtca
     3301 agcaccgcct ccagcccccg ggccttgacg ttctcgagca cctcggcggc ggcggcgtcg
     3361 acatcggccg gcgagcctcg ccgttcgttc aggaagctcc tgaaggcggc ggtgaaatcc
     3421 gggtctgtgg aacggaaacg acgcatgggc ggcttcatgg ccgttttcag cgcaaactca
     3481 agccttcatt gacgccgggg gcattccggc caggcaagct ggtccttgcc gatgtcgagc
     3541 gcggcgagca ctgcggtccg gccggcgtac ttgcgcctca gcgcggcctc gtcccgatcg
     3601 agccggtcgc agcgaagctc gcgaaccgcc cgccggatct gacggtcacg atccgcgtca
     3661 tacgcctctt ctccgcccca gtgcgcgcaa tccgcccagc gctgcatgaa gtccacgacg
     3721 tcccgcggcg tcgaacgctc cagtcggcag aggtctgcgt cggtctgctc ggccgaagcc
     3781 gcgccggaga cgacggccgc caggagcagc gcggtcagag cggggatcga tcgccgcatg
     3841 ccgcctctcc ttcagtcccc gtgcgtcggc gtccgcggcg tcggccaggg ggtcgagacg
     3901 tcggccagca cggcctcgag gcattccacc tcggccctca ggtcgccgcc gccggcgaag
     3961 gtgaacacca cggccccgcc cggcgcctcg cccggttcga agttcagggc cagaagctcg
     4021 accacggcgc ctttcgaatc ccgcctgagg ttgcggctgc gcaccgccat caccccgccc
     4081 agctgcaggc cgctgcgcac ccgctcatgg gtccggccgt cgcgctccca gcgatagcgg
     4141 ttgaaggcga cggtcagccg gcgcgccctc ggctcgaacg cgatgtcgcc gatcttcgcc
     4201 accgcgtcct gcagggcggc ggagatcacg gccaggtcct ctgcgtcctg ggccagcagg
     4261 cgaagcggcg cgggcgaggc catggtcagt cctcggcctc cggcgcgggg tcccccttga
     4321 tccggcgcac atcggcgccg caggcgccga gcttctcctc caggcgttcg aagccgcggt
     4381 cgaggtgata gacacgtccc acgaccgttt cgccgcgcgc cgcgaggccc gcgatgacca
     4441 ggctgaccga ggcccgcagg tcggtggcca tcacctgcgc gccttccagg tactcgacgc
     4501 cgcgcacgcg cgcctcgccg ccggagacgg tgatgtcggc ccccagccgc gccagttccg
     4561 gcgcgtgcat gaaccggttc tcgaagatgt gctcgcggat gacgctctcg ccctcggcca
     4621 gggtcatcag ggccatgaac tgggcctgca ggtcggtggc gaagccgggg tagactccgg
     4681 tgtcgatatc caccgccttc agccgcgcgc cgttgcggcg gatgatcacg ccgtcattgg
     4741 tgcgctcgac gccggccccg gcctcctcga tcttcagcag cagggcctcg atcaggtcgg
     4801 cgcgggtgtt ggtcaggcgc acctcgccgc cggccatggc cgcggccacc gcgtaggtgc
     4861 ccgcctcgat gcggtcggtg atcaccgcat gggtcgcgcc acccagcttc cggacgccgg
     4921 tgatgcggat ggtcggcgtg ccggccccct cgacccgggc gcccatcttg ttgaggcagt
     4981 cggccagatc gacgatctcg ggctcgcagg cggcgttggc cagaacggtc tcgccgcttg
     5041 ccagcacggc ggtcagcatg gcgtgttccg tggccccgac cgagacgaac ggaaactcga
     5101 tctccgcgcc cttcagaccg cgcggggcct gggcgtagac atagccttcg tgcaggtcga
     5161 tctgggcccc aagcgcctcc agcgccttga ggtgcaggtc gaccggccgc gcgccgatgg
     5221 tgcagccgcc cggcaggctg accttggcct ggccggtgcg ggcgatcagc gggcccagca
     5281 cattgaagct ggcgcgcatc tgccgcacca ggtcgtaggg cgcgaacgag gagacgatct
     5341 cgggcgtgtg cagcagggtc tcagacccgg ccggtccttc ccgctcgtcg accatggtcc
     5401 ccaggcgggt cagcagggtg ccaaggaacc gggtgtcagc cagccgcggc atgttggtca
     5461 ggtgaagcgg ctcttcggtg agcaggctgg cggtcatcag cttgatggcc gagttcttgg
     5521 cgccgctgat cggaatttct ccgtgcagcc gcgcgccgcc gacgatggcg atgcggtcca
     5581 ttcaggcccc tcgaaacgcc ggcagcctgt cgccccggcg gcttgtcaca tgtgagggcg
     5641 cggttctagc gggcgcaagc ccccgcgcaa gagtcactta tgcttgatgc ccggcctcgc
     5701 ccggacgacg gaacagcgtc acggggacgt acggaccgcc gtcggcgaac gccagcccca
     5761 gtttttccgt cgccagctcc agttcgacgt gcgcgatgaa cagcgcgtgc tcgatcgcct
     5821 gcatcggatc tacgccaaaa aaagcccgtc cgtgctcggc ccgatgctcg ggatccagcc
     5881 cgacgatccg catggcgcag gcccagtcgc ggctgtcctc gagccggaag acgtcggaga
     5941 tctcgacagt gacatcgaac ggttcgccct gtggaggccg ggccgcgcgc agatgacgga
     6001 cctcacggta gggcctgggc gccagccgcg cgcagttggc ctggtgtgtc attcgtcacc
     6061 cttgggcctg gcgccgtccc tgggcgcggc cttgcgccga cgcaggttgg cgcgcagggc
     6121 ctgggccagt ttcgcctccc ggtcggcctt ctcgggcggc ggcgcctgcg gtggattctt
     6181 catccggcaa gcagcgcatt gcacatggcg acgcgtcaag ccggattagg gcttcccctg
     6241 ccgcccgggt ttcgctatca ggcgcgcctc gtctttcgcc gccgtagctc agtggtagag
     6301 cgcatccttg gtaaggctga ggtcggcagt tcaatcctgc ccggcggcac catccctgaa
     6361 ccagagggaa gaagacgatg accagacccc gaaaagatgg cggtttccct ccggccgaca
     6421 tgccggcgcg gaccgttcat tcgggctggc tcgacctgca cctggtcgaa ctgaccaatc
     6481 ccgacgggtc gacctatgac cgatacgtcg aggatcacgg ccgcgccgcg gcggtcctgc
     6541 cctacaatcc gacgacccgc atggccctgc tggtgaccat gccgcgcgcc gccctgcgat
     6601 tcgccggcgg cggcgacgtg ctggaggccc cggccggcct gatcgatccc ggcgaaaccg
     6661 ccgccgactg cgcgcgtcgc gagctggtcg aggaggcggg gctggaggcc ggaacgcttg
     6721 agccggcggg ggcgatcatt ccttcaccgg gtatctcgac cgaacagatc gacctgtttc
     6781 tcgcccccta ccaggactcc cagcgcacgg gcgcgggcgg tggcctggac ggcgagaacg
     6841 agaacatcac cgtcgtcgag acgccgctcg atcggctttg ggcgctgatt gacggcgacg
     6901 ccctgtccga cgccaagacc cgcgtgctgg tgcagaccct gcggctgcgg cgaccggaac
     6961 tgttcagctg acccgcgcgg cagcggcgta gccggccagg gcggcgatgg cggtcacggc
     7021 ggcgccccag gcaaggtccg gcgccaccac ccggatcgga aagcccttga gggtggccag
     7081 gttggtcagg tcataggtgg cgtaggcgac caggccgaac agggcgccgt tcaggagcgc
     7141 ccgggtccag ccgccgccgt cgagcgccgg cttcaccgcg aagaacacca ccccgcccag
     7201 atagagggcg tagaaggcgg cggccgccgc catgttgggc ttctcgagca acagggcccc
     7261 gaattcccgc tggtagagcg tgcgggcgat cagccccagc cagagggcgt cgagccccag
     7321 gaagctgacg gcggcggcga cataggcggc gatccaggtc atggtcgggt ccctctcaga
     7381 cagctggatc tagtccgccg cgcggcgacg ccaaggccgg tcagccggcg ccgcgtcgcg
     7441 gcgtccggtc gccgaaccac ca
//
LOCUS       DAORNX010000097         7394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7411_length_7394_cov_8.415860, whole genome shotgun sequence.
ACCESSION   DAORNX010000097 DAORNX010000000
VERSION     DAORNX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_7394_cov_8.415860"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..764)
                     /locus_tag="PLO65_05940"
     CDS             complement(159..764)
                     /locus_tag="PLO65_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27822.1"
                     /translation="MVKVYFAPFARSLRILWTLEELNAPYEPVRVQFPPRQAQPDYLH
                     INPLGQIPTLIDGDSKLFESVGACEFVNETNGGDLAIAPGQPGRADYLQWLWYGEATL
                     MPPLGTMVRNTFGPEETRDAKALAEAKASLFERLALVEARLEGRQYLAGDRFTLADIS
                     VAYALNLGGMLRVAADYGPNTAAYFERMASRPAFKAAAAQA"
     gene            complement(844..2265)
                     /gene="rho"
                     /locus_tag="PLO65_05945"
     CDS             complement(844..2265)
                     /gene="rho"
                     /locus_tag="PLO65_05945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRD27823.1"
                     /translation="MSEDTHETPDAEVDTTVEVSTAAETAEAAAEAAEAAADGEGSEI
                     AAVVAELGLTRMSLQELKEKSPADLLALAEKLEVENANSMRKQDMMFAILKTVAEEGV
                     EISGSGTMEVLPDGFGFLRSPEANYLPGPDDIYVSPSQIRKFGIRTGDSVVGAIRAPR
                     EGERYFALTTVEQINFETPENVRHKVHFDNLTPLYPEERLKMEIEDPTLKDRSGRIID
                     IVAPLGKGQRCLIVAPPRVGKTVMLQNIAKSIAANHPECYLIVLLIDERPEEVTDMQR
                     TVKGEVISSTFDEPATRHVAVAEMVIEKAKRLVEHKRDVIILLDSVTRLGRAYNTVVP
                     SSGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNSEIILDRKVADKRIFPAIDILKSGTRKEELITPKDQLQKTYVLRRILNPMGAQDA
                     IEFLMDKLRQSKNNGDFFQSMNT"
     gene            complement(2508..2975)
                     /locus_tag="PLO65_05950"
     CDS             complement(2508..2975)
                     /locus_tag="PLO65_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRD27824.1"
                     /translation="MTDWLVAHFNLIRGLHIISVIAWMAGMLYLPRLYVYHVKAAPGS
                     ELDETFKVMELKLLRIIINPAMIATFTFGLLLIMADGSIRGWDFLLTPWMLTKLAGVT
                     FLSGWHGFLAAARRRFAANANTRPEKFWRMTNELPFLAAVAIVLSVTTKFLDH"
     gene            complement(2972..4006)
                     /gene="hemH"
                     /locus_tag="PLO65_05955"
     CDS             complement(2972..4006)
                     /gene="hemH"
                     /locus_tag="PLO65_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRD27825.1"
                     /translation="MSRKVAVVLFNLGGPDGPDAVRPFLFNLFNDPAIIGLPGILRTP
                     LAALISSRREASARANYAIMGGGSPLLPETRGQARALEAALADRAPDDEIRCFIAMRY
                     WKPLTEETAAEVAAWAPDEIVLLPLYPQYSTTTTGSSAQAWTRAYKGAGRMRTVCCYP
                     TLGGLVEAHARLIERAWAAANHAGPARLLFSAHGLPEKVIAGGDPYQAQIEATCAAVA
                     ARLGGTWDWKVCFQSRVGPMKWIGPSTDDEIRAASAAGLALVVTPIAFVSEHVETLVE
                     LDHEYGEMARSAGCPAYVRVPALGLEAGFIDSLADLALAALDRTGVEPAGGHACAGWS
                     KCPRTEGAAA"
     gene            complement(4003..5031)
                     /gene="hemE"
                     /locus_tag="PLO65_05960"
     CDS             complement(4003..5031)
                     /gene="hemE"
                     /locus_tag="PLO65_05960"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRD27826.1"
                     /translation="MTQPVIPSLLRVLDGETLERPPVWFMRQAGRYLPEYLALRATTP
                     DFISFCLNPEKAAEATLQPMRRFPFDAAIVFADILLLPQALGQEVWFEAGEGPRLGAL
                     PDLDTLAGQIEASTFKLLPVGETLTRVRAALEPDRALIGFAGGPWTVATYMIEGRSSD
                     RSKARTFAYEHPAEMDRLLDIIVQSTVRYLAMQVDAGAQVLKLFESWAEGLPEDLFER
                     LVCKPHRAILDGLAGLDVVAPVIGFPRGAGSLAQHYADTVGVDAVALDTAASAMLGRS
                     IQEKVPIQGALDPLLLRAGGAALDHRVDQLLEQWGDGPYIFNLGHGIILDTPIAHIER
                     VLKRITQE"
     gene            5544..6392
                     /locus_tag="PLO65_05965"
     CDS             5544..6392
                     /locus_tag="PLO65_05965"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRD27827.1"
                     /translation="MTATTRLATYFHIHLVSDSTGETLNAMARAVCARFTDILPIEHI
                     YALVRSPRQLDRALEEIAGSPGVVMHTIIDAVLRSALEEGCRRHDIPCIAALDPVVAA
                     MSRYLGAPISSRVGAQHALDTDYFNRMEALNYAMGHDDGQGGQDLEQADVVLVGVSRT
                     SKTPTCIYLAHRGVRAANHPLVPGAPLPPSLTSLKNTLIVGLIASPDRLIQIRRNRLL
                     SLNEQRESTYVDQDAVREEIIAARRLFERMGWPVIDVTRRSVEETAAAIINLLSGGRG
                     AVEVLG"
     gene            6389..6985
                     /locus_tag="PLO65_05970"
     CDS             6389..6985
                     /locus_tag="PLO65_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRD27828.1"
                     /translation="MSGVILASGSKARAQVLAGAGVAFEIAVAGVDEAAVKAGLLAQG
                     NGPRDVADALAELKAVRVSRRHPGVLVIGADQTLDLDGALFDKAESMAEARERLRHLR
                     GKTHKLHSAVVVARDGEVIWREVPAARLTMRPFTDAFLEAFLERGGESLLGSVGCYRL
                     EDDGVQLFSRIDGDYFTILGLPLMGLLDLLRRHGVLPL"
     gene            6982..>7394
                     /locus_tag="PLO65_05975"
     CDS             6982..>7394
                     /locus_tag="PLO65_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRD27829.1"
                     /translation="MSAITGAARVAGIIGRPVAHSLSPVLHNAWIEAAGLDAVYAPLA
                     PAADGFEALVTGLRGGVIVGVNVTIPFKEQALAIADDASDRARLAGAANLLLFHADGT
                     IFADNTDGLGMLAALAVQAPGFDPRAGPAVILGAGG"
BASE COUNT         1150 a         2466 c         2564 g         1214 t
ORIGIN      
        1 acagcccgcg tcgagcaccc gcaggccctc ccaggggccg gcgcgatcca gcagggcgcc
       61 cgtgaagggc gccatcgcct cggccagcac ggaaagccgc gcccgtcccg tttcgccacc
      121 ctcgatgatg tagctggacg acatgggcgc ggatccggtc aggcctgggc ggcggccgcc
      181 ttgaaggccg ggcggctggc catccgttcg aaataggcgg cggtattggg gccgtagtct
      241 gccgcaacgc gcagcatacc gcccaggttc agcgcatagg ccaccgagat gtcggccagg
      301 gtgaagcgat cgccggccag atactgccgc ccttcgaggc gggcctcgac cagggccagg
      361 cgttcgaaca ggctcgcctt cgcctcggcc agcgccttgg cgtcccgtgt ctcctcgggc
      421 ccgaaggtgt tgcgcaccat ggtcccgagc ggcggcatca gggtcgcctc gccataccag
      481 agccattgca gatagtcggc gcggcccggc tggccgggtg cgatggccag atcgccgccg
      541 ttggtctcgt tgacgaattc acaggcgccg accgactcga acagcttgga gtcgccgtcg
      601 atcagcgtcg ggatctggcc cagcggattg atgtgcagat agtcgggctg cgcctgcctg
      661 ggtggaaact gcacccgcac cggctcgtag ggcgcgttca gctcttccag ggtccagagg
      721 atgcgcagcg atcgcgcgaa cggcgcgaaa tagaccttca ccatgtgatc tctcccgttt
      781 tctcgtcggg aggggacgct acgccccttt ccccttgcgg ggaaaggggc gcggtcaatg
      841 tcgtcaggta ttcatcgact ggaagaaatc gccgttgttc ttcgactgac gcagcttgtc
      901 catcaggaac tcgatcgcgt cctgggcgcc catcgggttg agaatccggc gcaggacgta
      961 ggtcttctgc agctggtcct tcggggtgat cagctcttcc ttgcgagtgc ccgacttgag
     1021 gatgtcgatg gccgggaaga tgcgcttgtc ggcgaccttg cggtcgagaa tgatttccga
     1081 gttcccggtg cccttgaact cttcgaagat cacctcgtcc atccgcgagc ccgtgtcgat
     1141 cagggccgtg gcgatgatgg tcaggctgcc gccctcctcg acgttgcgcg cggcgccgaa
     1201 gaagcgcttg ggccgctgca gggcgttggc gtcgacaccg ccggtcagca ccttgcccga
     1261 cgaggggacc acggtgttgt aggcgcggcc cagccgggtg accgagtcca gcaggatgat
     1321 cacgtcgcgc ttgtgctcga ccaggcgctt ggccttttcg atgaccatct cggccacggc
     1381 cacgtggcgc gtggccggct cgtcgaaggt cgaggagatg acctcgccct tcaccgtgcg
     1441 ctgcatgtcg gtgacttctt ccggccgctc gtcgatcagc agcacgatca ggtagcattc
     1501 ggggtggttg gcggcgatcg acttggcgat gttctgcagc atcaccgtct tgccgacccg
     1561 cggcggggcg acgatcaggc agcgctggcc tttgccgagc ggggcgacga tgtcgatgat
     1621 ccggccggag cggtccttca gcgtcgggtc ctcgatttcc atcttcagcc gctcctcggg
     1681 gtagaggggc gtcaggttgt cgaaatggac cttgtgacgg acgttctcgg gcgtctcgaa
     1741 attgatctgc tcgacggtgg tcagggcgaa gtagcgctcg ccctcgcgcg gggcgcggat
     1801 ggcgcccacg acgctgtcgc cggtgcggat gccgaacttc cggatctgcg acgggctgac
     1861 gtagatatcg tcggggccgg gaaggtagtt ggcttcgggg ctgcgcagga agccgaagcc
     1921 gtcgggcagg acctccatgg tgccggatcc cgagatctcg acgccttcct cggcgacggt
     1981 cttgaggatc gcgaacatca tgtcctgctt gcgcatggag ttggcgttct cgacctcgag
     2041 cttttcggcc agggccagca ggtcggccgg ggatttctcc ttcagctcct gcagggacat
     2101 gcgggtcagg cccagttcgg ccacgaccgc ggcgatctcg gagccctcgc cgtcggcggc
     2161 tgcctccgcg gcctcggcgg cggcttcagc cgtttcggcg gcggtcgaga cctcgacggt
     2221 cgtgtcgact tcggcgtcgg gggtttcgtg ggtgtcttca gacatggata gactcggggc
     2281 gtgcgggcgc gggcgcccgt ctttcgaaac ttgatccgtt caggcccgcg aggacctcga
     2341 gcggcgatgg tctgcgccgg caagggcgcg gggtgttcgt tctggggggc cgtcccaggg
     2401 gcgcaccgtg atcgggcgcg ggatcggcga agaggcggga acgccgggtg ttacgcccga
     2461 cgtctgggag gaggggaacc acaaccccgc gtcggggtca agaagtccta gtggtcgaga
     2521 aacttggtcg tcaccgacag gacgatggcc acggccgcca ggaacggcag ctcgttggtc
     2581 atccgccaga acttctcggg ccgggtattg gcgttggcgg cgaacctgcg gcgcgcggcg
     2641 gcgaggaacc cgtgccagcc ggagaggaag gtcacgccgg ccagcttggt cagcatccag
     2701 ggcgtcagca gaaagtccca gccacgaatg ctgccgtcgg ccatgatcag cagcaggcca
     2761 aaggtgaagg tcgcgatcat cgccgggttg atgatgatgc gcagcagctt gagctccatc
     2821 accttgaagg tctcgtcgag ttcagacccc ggcgccgcct tcacatgata gacgtagagc
     2881 cgcggcaggt agagcatccc cgccatccag gcgatgacgc tgatgatatg cagcccacgg
     2941 atcagattga aatgagcgac cagccagtcg gtcatgccgc ggctccttcc gttctcggac
     3001 atttcgacca accggcgcag gcatgccctc cggcgggctc gacgccggtc cggtccaggg
     3061 cggccagggc caggtcggcc agactgtcga tgaatccggc ctccaggccc aacgccggga
     3121 cacgcacata ggcggggcaa ccggcggagc gcgccatctc gccgtactca tggtccagct
     3181 cgaccagggt ctccacgtgt tcggagacga atgcgatcgg cgtgacaacc agggccagcc
     3241 cggcggcgct ggcggcgcgg atctcgtcat cggtcgaggg gccgatccac ttcatcggcc
     3301 cgacgcggct ctggaagcag accttccagt cccaggtccc gcccagcctg gcggccacgg
     3361 ccgcgcaggt cgcctcgatc tgcgcctggt agggatcgcc tcccgcaatc accttctccg
     3421 gcagtccgtg ggccgaaaac agcagacggg cgggcccggc atggttcgcc gccgcccacg
     3481 cccgctcgat cagtcttgca tgggcttcga ccagtccgcc cagcgtggga tagcagcaga
     3541 ccgtccgcat ccggccggcg cccttgtagg ccctggtcca ggcctgggcc gacgatcccg
     3601 tggtggtggt cgagtactgc ggatagagcg gcagcagcac aatctcgtcg ggggcccagg
     3661 cggccacctc ggcggccgtc tcttcggtca gcggcttcca gtagcgcatg gcgatgaagc
     3721 agcggatctc gtcgtcgggc gcccggtcgg ccagtgcggc ttcgagcgcc cgcgcctgtc
     3781 ctcgggtttc cggcaacagc ggcgagcccc cgcccatgat ggcgtaattg gccctggccg
     3841 acgcctcgcg ccgactggag atcagggccg cgagcggcgt gcgcaggatg ccgggcaggc
     3901 cgatgatcgc cggatcgttg aacagattga acaggaacgg ccgcacggcg tccggcccgt
     3961 ccggcccgcc gaggttgaac agcaccacgg cgaccttgcg gctcattcct gggtgatccg
     4021 cttcagcacc cgctcgatat gggcgatcgg ggtgtccaga ataatgccgt ggccgagatt
     4081 gaagatgtac ggcccgtcac cccactgctc gagcagctgg tcgacccgat ggtccagggc
     4141 ggccccgccg gcgcgcagca gcagcggatc cagagcgccc tggatcggca ccttctcctg
     4201 gatgctgcgg ccgagcatcg ccgaggcggc ggtgtcgagc gccacggcgt ccacgcccac
     4261 cgtatcggca tagtgctgcg ccaggctgcc cgcgccgcgc gggaagccga tcaccggcgc
     4321 gaccacgtcc agcccggcca ggccatcgag gatcgcccgg tgcggcttgc acaccagccg
     4381 ctcgaacagg tcctccggca agccctcggc ccagctctcg aacagtttga gcacctgggc
     4441 gccggcgtcg acctgcatgg ccagatagcg caccgtcgac tggacaatga tgtccagcag
     4501 ccggtccatc tccgccgggt gctcgtaggc gaaggtgcgg gccttgctgc ggtccgagga
     4561 gcggccctcg atcatgtagg tggccacggt ccagggcccg ccggcaaagc cgatcagcgc
     4621 ccgatcgggc tccagcgcgg cgcgcacgcg ggtcagggtc tccccgacgg gcagcagctt
     4681 gaaggtcgag gcctcgatct ggcccgccag cgtgtccaga tcgggcaggg cgccgagcct
     4741 gggtccctcg ccggcctcga accagacctc ctggcccagc gcctggggca gcagcaggat
     4801 gtcggcgaac acaatggccg cgtcgaatgg aaaacgccgc atcggctgca gcgtggcttc
     4861 cgccgccttc tcgggattga ggcagaagct gatgaagtcc ggcgtcgtgg cccgcaaggc
     4921 cagatactcg gggagataac ggccggcctg gcgcatgaac cagacgggcg ggcgctccag
     4981 cgtctcgccg tcgagcacgc gcagcaggct tgggatcacg ggttgggtca tcgtgggtct
     5041 tgataggcca catggcccgg gaggcaagtc cacggctcct ctttcattcc ttaataagag
     5101 attaagaagg ttggggtgga agaggtaggg ccggggcgcc gcgtgcacaa acggcgagag
     5161 cggccggaga ttcagcagtc tggcccaggt catgcccgtg ctcttcccca accggaaaac
     5221 cgtcgtgtgg attgcgggga caaggcaggc agaccccgga aatccttaat gaaaggttaa
     5281 gacggttcag cggaaaacgg gccggaatcc ggtgttcgct agacccaggg gaaaacccgt
     5341 caggccgaga ccggtcacgg cgagctgtct ggggatggcc ataccaaaag ccacgccggt
     5401 catccccgcg gcgcccagcc cggccatacc gccgcgcggc cctgtgcatg gccggccaaa
     5461 cggcgccctt tcgggttaac ggttcgttaa cgacgtatcc acaggcttgg ttccgggccc
     5521 cgcggcgccc tatggatgag gcgatgaccg cgaccacccg cctggcgacc tatttccaca
     5581 tccatctcgt gtccgattcc acgggcgaga cgctcaacgc catggcccgc gcggtctgcg
     5641 cacggttcac cgacatcctg ccgatcgagc acatctacgc cctggtccgg tctccgaggc
     5701 agctggatcg cgcccttgag gagatcgccg gctcgccggg ggtggtgatg cacacgatca
     5761 tcgacgcggt cctgcggtcc gcgctcgagg aagggtgccg ccggcacgac attccctgca
     5821 tcgccgccct cgatcccgtg gtggcggcca tgtcgcgcta tctcggtgcg ccgatctcca
     5881 gccgcgtcgg ggcgcagcac gcgctggaca ccgactattt caaccggatg gaggcgctca
     5941 actacgccat gggccacgac gatggccagg gcggccagga cctggaacag gccgacgtcg
     6001 tgcttgtcgg ggtgtcacgc acctccaaga cgccgacctg catctacctt gcccatcggg
     6061 gcgtgcgcgc cgccaatcac cccctggtcc ccggcgcgcc gctgccgccg tcgctgacca
     6121 gcctgaagaa caccctgatc gtcggcctga tcgcttcgcc cgaccgactg atccagatcc
     6181 gccgcaaccg gttgctgtcg ctgaacgagc agcgtgaaag cacctacgtc gaccaggatg
     6241 cggtgcgcga ggagatcatc gccgcgcgcc gtctgttcga acgcatgggc tggccggtca
     6301 tcgacgtgac ccggcgctcg gtcgaggaaa cggccgcggc gatcatcaac ctgctgtcgg
     6361 gcggccgggg cgccgtcgag gtgctcggat gagcggcgtg atcctggctt cgggcagcaa
     6421 ggcgcgcgcc caggtgctgg ccggggccgg cgtggccttc gagattgccg tcgccggcgt
     6481 tgacgaagcc gccgtcaagg ccgggctgct ggcgcagggc aacggcccgc gcgacgtcgc
     6541 cgacgccctg gcggaactga aggccgttcg tgtttcgcgc agacatcctg gcgtcctggt
     6601 catcggcgcg gaccagacgc tcgatctcga cggcgccctg ttcgacaagg ccgagtccat
     6661 ggcggaggcg cgggaacggc tgcggcatct gcgcggcaag acccacaagc tgcattccgc
     6721 cgtggtcgtc gcccgggacg gcgaggtgat ctggcgggag gttcccgccg cccgcctgac
     6781 catgcgcccg ttcaccgacg ccttcctcga ggcctttctc gaaaggggcg gggagagcct
     6841 tctgggatcg gtcggctgct accggctgga ggatgacggg gtgcagctgt tctcgcgcat
     6901 cgacggcgac tatttcacca tcctcggcct gccgttgatg ggcctgctcg acctgctgcg
     6961 gcggcatgga gtgctgccgt tatgagcgcg atcacgggcg cggcgcgggt ggccgggatc
     7021 atcggccgcc ccgtcgccca ttcgctcagc cccgtgctgc acaacgcctg gatcgaggcc
     7081 gccggtctcg acgcggtcta tgcgccgctg gcccccgcgg cggacgggtt cgaggcgctg
     7141 gtcaccggcc tgcgcggcgg tgtgatcgtc ggcgtcaatg tcaccatacc cttcaaggag
     7201 caggcgctgg ccatcgccga cgacgccagc gaccgcgcgc gcctggccgg agccgccaac
     7261 ctgctgctgt ttcacgccga cgggaccatc ttcgccgaca ataccgatgg tctgggcatg
     7321 ctcgcggcgc tggcggtcca ggcgccgggc ttcgatcccc gggccggccc ggcggtgatc
     7381 ctcggggccg gcgg
//
LOCUS       DAORNX010000098         7338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7492_length_7338_cov_8.456680, whole genome shotgun sequence.
ACCESSION   DAORNX010000098 DAORNX010000000
VERSION     DAORNX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_7338_cov_8.456680"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1268
                     /locus_tag="PLO65_05980"
                     /pseudo
     CDS             1..1268
                     /locus_tag="PLO65_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671826.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE family protein"
     gene            1275..2789
                     /locus_tag="PLO65_05985"
     CDS             1275..2789
                     /locus_tag="PLO65_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRD27830.1"
                     /translation="MFGKRSDGSQAPERRAPPPAPEGAPIATRPQPAAGPRPDAPPAQ
                     NPATAAPGPQRPAAKPMKGLDQLRSAQSPTAQIVREQSDYYHATKTTIFNALLNTIDL
                     SQLAQLDQKSAAEEIKDIVAELVAIKNVQMSVAETEHLVQDIINDVLGYGPLEPLLSR
                     DDIADVMVNGANRVFIEVGGKVQLTNVRFRDNAQLMNICQRIVSQVGRRVDESSPICD
                     ARLPDGSRVNVIAPPLALDGPTLTIRKFKKDKLTMKDLVDFKSISPEGARVLGVIGAC
                     RCNIVISGGTGSGKTTLLNTMTAFIDPTERVITCEDAAELQLQQPHVVRLETRPPNLE
                     GQGMITMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREA
                     ISRMESMITMGGYGLPSKTIREMITGSVDVIIQAARLRDGSRRITHITEVVGLEGDVI
                     VTQDLFVYEMTGEDDNGRVQGKFRSTGIARPRFWDRARYYGLERELAEALDAAE"
     gene            2793..3773
                     /locus_tag="PLO65_05990"
     CDS             2793..3773
                     /locus_tag="PLO65_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD27831.1"
                     /translation="MLMIVVAALAFVTIAGLGFVFAGGDSERARATKRAQALVSGGTG
                     ATRVRNARAQANSPEARRKAILKTLKDQDRQQKKATLSIAARLQQAGLAFTEKQFWIA
                     SAVFGLVIGALSLPFGKSPLIALGLAFAAGLGLPRWIVGFIAKGRMKKFTEAFSDAID
                     IIVRGIKSGLPVHDCLKIIGKECPEPLAGEFRRLVENVGMGMAMDQALDKMYERMPTN
                     ELRFFAIVLAIQQKTGGNLAEALNNLSVVLRGRKLMREKIKALSSEAVASSFIIGSLP
                     PGVVTLISITSPKYMAPLFTDPRGHLMLLGAVFWMSLGIFVMRKMINFKF"
     gene            3784..4776
                     /locus_tag="PLO65_05995"
     CDS             3784..4776
                     /locus_tag="PLO65_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD27832.1"
                     /translation="MDLVQFLTDPDNLLTGFVAVVAFATIVTLVRPILFTSSLESRLK
                     SVSNRREELRRRSREEMAKRASGGSLRQTDEGLYKKVVDRLQLSRLLEDPKVAEKMAQ
                     AGFRGPRPISAFYFFRFLMPFVFAMAAAFYLFVVNDFGLPSMSRLTICVGALLLGYYA
                     PNVYISNVAQKRRESIVAAFPDSLDLLLICVESGMSIEAAIQKVSQEIGGTSIELAEE
                     LTLVSAELSYLPERRQAYEGLAKRTNHPGIKAVATAMIQAERYGTPLGAALRVMSKEN
                     RELRLSAAEKTAAALPAKLTVPMILFFLPVLFVMILGPAIITVQDTLAKQHSGG"
     gene            complement(4773..5993)
                     /locus_tag="PLO65_06000"
     CDS             complement(4773..5993)
                     /locus_tag="PLO65_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRD27833.1"
                     /translation="MNALTIDREGWTAAGDALLHDAIELRRAIHREPELGLHLPKTTE
                     KVLKALEGLPLEIRQGPSTTGVLAILRGSPNGRTVLLRGDMDALPLTEDTGLDFSSGT
                     PGAMHACGHDTHVAMLAGAARALCAKKDQLAGTVMFMFQPGEEGHHGARFMLDDGLID
                     PLPDAAFALHISPNMPTGVFASRTGALLASSDVIEIRVLGAGGHASMPHDAVDPIPVA
                     CEIVTALQTMVTRTISVFDPAVITIANVVAGTTNNIIPEDARLKGTLRTLSERARETA
                     HAGIRRVAEHVAAAHGCTAEVEIIRGFPVTVCSGREVDLAERTAKALYGEGAWMTMPN
                     PMMGAEDFAYVLQKVPGLMAFLGAAPVGVGGDYRSCCALHSNRMTLDEQVMARGIAMH
                     CAYAEAVLAGALAD"
     gene            6059..7039
                     /locus_tag="PLO65_06005"
     CDS             6059..7039
                     /locus_tag="PLO65_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRD27834.1"
                     /translation="MSTVTFADIEAAAARIAGVAVMTPLLESPALNERLGRRVLIKPE
                     TLQRVGAFKFRGAYNRLVQLSPDERARGVVAFSSGNHAQGVALAAKLLGVPAIIVMPH
                     DAPAKKVEATRGHGAEVRIYNRYTEDRGAIAAEIAATRGSTIVPPYDDPHIVAGQGTV
                     GLELMRQAKALGLTPDCVITPLGGGGLLSGVSLAVKHLSPDTAVVGVEPEGYDDVARS
                     LKSGVFETNSPEARTLCDALQTPTPGKITFEIMRRTVADVAVVTDAEVAEAMRLAFET
                     LALVVEPGGCVGLAALLAGKVRPAGAVTGLVLSGGNVDPGLYARVLAGEV"
     gene            7039..>7338
                     /locus_tag="PLO65_06010"
     CDS             7039..>7338
                     /locus_tag="PLO65_06010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRD27835.1"
                     /translation="MIAGFVPPQTPLLVVLRSALDHNLATMQSRCDRAGVRLRAHGKT
                     HKCSTLGRLQVAGGAVGLCAQTVGEAEAFVKGGVRDVLVNGVAVLRDGAHTGATPG"
BASE COUNT         1143 a         2607 c         2512 g         1076 t
ORIGIN      
        1 cgggtcggac ctggcggccc gccggacctg acggccgccg gcgacggcgc gatgggcgac
       61 gtcagcgtgc cgcgcatctc gatccatgtc ttctgcgccc gccccgatac ggccgagctg
      121 atcgagaagg ccgcggccga ccggcgcatg gcccgggcgg ccaccacggt gcagccgggc
      181 ggcatccgcg ccgccatcga gcagtaccag aaccagccga ccccctcgct ggtcatcgtg
      241 gagagccttg atccggcgcc ggaactgctc gcccagctgg accggctggc cgaggtctgc
      301 gacccgggca ccaaggtcat gaccatcggg tcggccaacg acatcgccct ctatcgcgag
      361 ctgatgcgcc gcggggtcag cgaatacctg gtgccgccgc tgcggccgct gcagatcatc
      421 cgcgcgatca ccgggctgta cgccgatccg gccgcgccct tcgccggccg ccagatcgcc
      481 gtggtcggcg cgcgcggcgg cgtcggggct tcgaccatcg cccacaatct cgcctggtcg
      541 ctggccgagc ggatgcaggc cggcacggtg ctggccgact tcgatctgcc gttcggcacg
      601 gccgggctca acttcaacca ggacccgcta cagggcatcg ccgacgccct gggccagcca
      661 gagcggctcg acccggtgct gatggaccgg atgatggcgc gggtcaccga ccgtctgtcg
      721 ctgttcgccg cgccggccac cctggagcag gatttcgaca tccatccgga cgccttcgag
      781 gaggtggccc agaagatccg cgccgccgct ccgttcgtgg tgctcgacct gccgcacgcc
      841 tggagcggct ggaagcgcaa gatgctgatc gcctccgacg acatggtcat cgtcgcctcg
      901 ccggatctcg cctcgctgcg caacgccaag aacatgatcg acctggtccg ccaggcgcgc
      961 ccgaacgacg ccccgccccg gctggtgctc aatcaggtcg gcgtgcccgg ccgcccggag
     1021 atcccggtca aggacttcgg cgaagcgctc ggcgtcgcgc cgtccgtggt cctgccgttc
     1081 gaacccaagg ccttcggcca ggcggccaat cacggccaga tgctgggcga ggtggcgccc
     1141 aagtccaagg cggccgaagg cattgaccag ctggcccggc tggtcgctcc ccgccacgtt
     1201 cctcccgccg cgatcccccg ccgccgcaga aatcgtcgct cctcggcggc ctgttcagaa
     1261 agaagtagcc cagcgtgttc ggcaaaaggt ccgatggcag tcaggctccg gagcgaaggg
     1321 cgcccccgcc cgcgcccgaa ggcgcgccca tcgccacgcg accccagccc gcggccggtc
     1381 ccaggcccga cgcccctcca gcccagaatc ccgccacggc ggcgcccgga ccgcagcgcc
     1441 cggccgccaa gccgatgaag gggctggacc agctgcgttc ggcccagagc ccgaccgcgc
     1501 agatcgtccg tgagcagagc gactactacc acgccaccaa gaccacgatc ttcaacgccc
     1561 tgctcaacac catcgacctg agccagctgg cccagctgga ccagaagtcg gcggccgagg
     1621 agatcaagga catcgtcgcc gagctggtgg cgatcaagaa tgtgcagatg tcggtcgccg
     1681 agaccgagca tctggtccag gacatcatca acgacgttct gggctacggg ccgctggagc
     1741 cgctgctgtc gcgcgacgac atcgccgacg tgatggtcaa cggggccaac cgggtgttca
     1801 tcgaggtcgg cggcaaggtg cagctgacca acgtgcgctt ccgcgacaac gcccagctga
     1861 tgaacatctg ccagcggatc gtcagccagg tcggccgccg tgtcgacgag agctcgccga
     1921 tctgcgacgc ccgactgccg gacggctccc gggtcaacgt catcgccccg ccgctggcgc
     1981 tggacggccc gacgctgacc attcgcaagt tcaagaagga caagctgacg atgaaggatc
     2041 tggtggactt caagtccatc agtccggaag gcgcgcgggt gctgggcgtg atcggcgcct
     2101 gccgctgcaa catcgtcatc tccggcggca cgggctcggg caagaccacc ctgctcaaca
     2161 ccatgaccgc cttcatcgac cccaccgagc gggtgatcac ctgcgaggac gcggccgagc
     2221 tgcagctgca gcagccgcat gtggtgcggc tggagacccg gccgccgaac ctcgagggcc
     2281 agggcatgat caccatgcgt gacctggtga agaactgcct gcgcatgcgg cccgagcgga
     2341 tcatcgtcgg cgaggtgcgc ggccccgagg ccttcgacct gctgcaggcg atgaacaccg
     2401 gccacgacgg ctcgatgggc acgctgcacg ccaacagccc ccgcgaagcc atcagccgga
     2461 tggaatcgat gatcaccatg ggcggctacg gcctgccctc gaagaccatc cgcgagatga
     2521 tcaccggctc ggtggatgtg atcatccagg ccgcccgcct gcgcgacggc agccgccgca
     2581 tcacccacat caccgaggtg gtcggcctcg agggcgacgt gatcgtcacc caggacctgt
     2641 tcgtctacga aatgaccggc gaggacgaca atggccgggt gcagggcaag ttccgctcca
     2701 ccggcatcgc ccggccgcgc ttctgggacc gcgcccgcta ctacggactt gagcgcgagc
     2761 tggccgaagc cctcgatgcg gcggagtagg cgatgctgat gatcgtcgtc gccgccctgg
     2821 cttttgtcac catcgccggc ctcggcttcg tgttcgcggg cggcgattcg gaacgcgcgc
     2881 gggcgaccaa gcgggcccag gccctggtca gcggcggaac cggcgcgacc cgggtgcgca
     2941 acgcccgggc ccaggccaat tcgccggagg cccgccgcaa ggccattctc aagaccctca
     3001 aggaccagga ccggcagcag aagaaggcca ccctgtcgat cgccgcgcgc ctgcagcagg
     3061 ccggcctggc cttcaccgaa aagcagttct ggatcgccag cgccgtgttc ggcctggtca
     3121 tcggcgccct gtccctgccg ttcggcaagt cgccgctgat cgcgctgggc ctggccttcg
     3181 ccgccggcct gggcctgccg cgctggatcg tcggcttcat cgccaagggg cggatgaaga
     3241 agttcaccga ggccttttcc gacgccatcg acatcatcgt ccggggcatc aagtccggcc
     3301 tgccggtgca tgactgtctg aagatcatcg gcaaggaatg ccccgagccg ctggccggcg
     3361 agttccgccg cctggtcgag aacgtcggca tgggcatggc catggatcag gccctggaca
     3421 agatgtacga gcgcatgccg accaacgagc tgcgcttctt cgccatcgtc ctggcgatcc
     3481 agcagaagac cggcggcaac ctggccgagg cgctgaacaa cctgtcggtc gtgctgcgcg
     3541 gccgcaagct gatgcgcgag aagatcaagg ccctgtcgtc ggaggcggtc gcctcgtcgt
     3601 tcatcatcgg cagcctgccg ccgggcgtgg tcaccctgat ctcgatcacc tcgcccaaat
     3661 acatggcgcc gctgttcacc gacccgcgcg gccacctgat gctgctcggc gcggtgttct
     3721 ggatgagcct tggcatcttc gtgatgcgca agatgatcaa cttcaagttc tgagggcgga
     3781 ccgatggatc tcgtccagtt cctcaccgac cccgacaatc tgctgacggg cttcgtcgcg
     3841 gtggtggcct tcgccaccat cgtcaccctg gtccggccga tcctgttcac ctcgagcctc
     3901 gaaagccgcc tcaagtcggt gtcaaaccgg cgcgaggagc tgcgcaggcg gtcgcgcgag
     3961 gagatggcca agcgcgcctc gggcggcagc ctgcggcaga cggacgaggg gctgtacaag
     4021 aaggtcgtcg accggctgca gctgtcgcgg ctgctggagg accccaaggt cgcggagaag
     4081 atggcccagg ccggcttccg cggcccgcgg ccgatcagcg ccttctactt cttccgcttc
     4141 ctgatgccgt tcgtcttcgc catggcggcg gccttctacc tgttcgtggt caacgacttc
     4201 ggcctgccga gcatgagccg gctgacgatc tgcgtcggcg ccctgctgct cggctactac
     4261 gcgcccaacg tctacatcag caacgtggcc cagaagcggc gcgaatcgat cgtcgcggcc
     4321 ttccccgact cgctcgacct gctgctgatc tgcgtcgaga gcggcatgtc gatcgaggcg
     4381 gcgatccaga aggtcagcca ggagatcggc ggcacctcga tcgagctggc cgaggaactg
     4441 accctggtca gcgccgagct cagctacctg cccgagcgcc gccaggccta cgagggcctg
     4501 gccaagcgga ccaaccatcc gggcatcaag gccgtggcca cggcgatgat ccaggccgag
     4561 cgctacggca cgccgctcgg cgcggcgctg cgggtgatgt ccaaggaaaa ccgcgagctg
     4621 cgcctgtcgg ccgccgagaa gacggccgcc gccctgccgg ccaagctgac cgtgccgatg
     4681 atcctgttct tcctgccggt gctgttcgtg atgatcctgg ggcccgcgat catcaccgtg
     4741 caggacaccc tggccaagca gcactcgggc ggctagtccg ccagcgcgcc cgccagcacc
     4801 gcctcggcat aggcgcagtg catggcgatg ccgcgcgcca tgacctgctc gtcgagggtc
     4861 atccggttgg agtgcagcgc gcagcaggac cggtagtcgc cgccgacccc gacgggcgcg
     4921 gcgcccagga aggccatcag gcccggaacc ttctgcagca cataggcgaa gtcctcggcg
     4981 cccatcatcg ggttgggcat ggtcatccag gcgccctcgc catagagggc cttggcggtc
     5041 cgctcggcga ggtcgacctc gcggccggag cagacggtga ccgggaagcc gcggatgatc
     5101 tccacctcgg ccgtgcagcc gtgggcggcg gcgacatgtt ctgcgacgcg acggatgccg
     5161 gcgtgggccg tctcccgcgc ccgctccgac agggtccgca gcgtgccctt caggcgcgcg
     5221 tcctcgggga tgatgttgtt ggtcgtgccg gcgacgacgt tggcgatggt gatgacggcc
     5281 gggtcgaaca ccgagatggt ccgggtgacc atcgtctgaa gcgcggtgac gatttcgcag
     5341 gcgaccggga tcgggtcgac ggcgtcgtgc ggcatcgagg cgtggccccc ggcgccgagc
     5401 acgcggatct cgatcacgtc ggacgaggcc agcaaagcgc ccgtccggct ggcgaacacc
     5461 ccggtcggca tgttgggcga gatgtgcagc gcgaaggcgg cgtcgggcag cgggtcgatc
     5521 agcccgtcgt cgagcatgaa gcgggccccg tggtggccct cctcgccggg ctggaacatg
     5581 aacatcaccg tcccggcgag ctggtccttc ttcgcgcaca gggcccgcgc cgcaccggcc
     5641 agcatggcca catgggtgtc atggccgcag gcgtgcatcg cgccgggcgt tcctgacgag
     5701 aagtccaggc cggtgtcctc ggtcagcggc agggcgtcca tgtcgccgcg cagcagcacg
     5761 gtgcggccgt tgggcgagcc gcgcaggatg gccagcacgc cggtggtcga cggtccctgg
     5821 cggatctcca gcggcagccc ctcgagcgcc tttagcacct tctccgtcgt cttcggcaga
     5881 tgcagcccca gctccggctc gcgatggatc gcgcggcgca gttcgatggc gtcgtgcagc
     5941 agcgcgtcgc cggcggcggt ccagccttcg cggtcgatgg tcagggcgtt cataaatcga
     6001 ctccggatgt gatcgcgcca acgtcgccct ttttgtcgca tcggtctagg acaggcgcat
     6061 gagcaccgtg accttcgccg atatcgaagc cgccgccgcc cggatcgcgg gcgtcgcagt
     6121 gatgacgccg ctgctggaaa gcccggccct caacgaacgc ctcggccgcc gggtgctgat
     6181 caagcccgag accctgcagc gggtcggcgc cttcaagttc cgcggcgcct acaaccggct
     6241 ggtccagctg tcgccggacg agcgggcgcg cggggtggtg gccttctcgt ccggcaacca
     6301 cgcccagggc gtggccctgg ccgcgaagct gctgggcgtc ccggcgatca tcgtcatgcc
     6361 gcacgacgcc ccggccaaga aggtcgaggc cacgcgcggc catggcgccg aggtgcgcat
     6421 ctacaatcgc tacaccgagg accgcggggc gatcgcggcc gagatcgccg ccacccgggg
     6481 ctcgaccatc gtgccgccgt acgacgaccc gcacatcgtc gcgggccagg gcacggtggg
     6541 actcgagctc atgcgccagg cgaaggcgct gggcctgacg cccgactgcg tgatcacccc
     6601 gctcggcggc ggcggcctgc tctcgggcgt cagcctggcg gtgaagcatc tgtcgccgga
     6661 cacggcggtg gtgggggtcg agccggaagg ctacgacgac gtggcgcggt cgctgaagtc
     6721 gggcgtcttc gaaaccaaca gccccgaagc ccgcaccctc tgcgacgccc tgcagactcc
     6781 gacgcccggg aagatcactt tcgaaatcat gcggcggacc gtcgccgacg tagccgtggt
     6841 cacagacgcc gaggtggccg aggcgatgcg gctggccttc gaaaccctgg ccctggtggt
     6901 cgagcccggc ggctgtgtcg gcctggccgc cctgctggcc ggcaaggtgc gaccggcggg
     6961 cgcggtcacc ggcctggtcc tgtcgggcgg caatgtcgat ccgggcctct atgcccgcgt
     7021 gctggcggga gaggtctgat gatcgccggc ttcgtcccgc cccagacgcc gctgctggtg
     7081 gtcctgcgct cggccctcga ccacaacctg gccaccatgc agagccgctg cgaccgggcc
     7141 ggcgtccggc tgcgggcgca cggcaagacc cacaaatgct cgaccctcgg ccggctgcag
     7201 gtggccggcg gggcggtcgg cctgtgcgcc cagacggtcg gcgaggccga ggccttcgtg
     7261 aagggcggcg tgcgcgacgt gctggtcaac ggcgtggccg tcctgcgcga cggcgcacat
     7321 acgggcgcga cgccgggg
//
LOCUS       DAORNX010000099         7331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7504_length_7331_cov_9.450522, whole genome shotgun sequence.
ACCESSION   DAORNX010000099 DAORNX010000000
VERSION     DAORNX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7504_length_7331_cov_9.450522"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLO65_06015"
     CDS             complement(<1..722)
                     /locus_tag="PLO65_06015"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRD27836.1"
                     /translation="MVQLALPKNSRIGKGRHYPAPQGAKRVKTFRIYRYDPEGSENPR
                     WDTYDVDVDACGPMLLDVLIHIKNTVDPTLTFRRSCREGVCGSCAMNIGGRNTLACTH
                     GWEDVPGKEIQISPLPSQPVVKDLVPDLTLFYAQYASIEPWLHTETPEPRTEWKQSVE
                     DRSKLDGLYECILCACCSTSCPSYWWNGEKYLGPATLLHAYRWLIDSRDEATGERLDA
                     LEDPFKLYRCHTIMNCAQVCPKG"
     gene            complement(730..2517)
                     /gene="sdhA"
                     /locus_tag="PLO65_06020"
     CDS             complement(730..2517)
                     /gene="sdhA"
                     /locus_tag="PLO65_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HRD27837.1"
                     /translation="MPAYEFIDHRFDVVVVGAGGSGLRAAVGCAQAGLKTACVTKVFP
                     TRSHTVAAQGGISASLGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYLTRNAPAAVYEL
                     EHWGVPFSRTPEGKIYQRAFGGMTRNYGEAPIQRTCAAADRTGHAMLHTMYGQALSKD
                     VEFFIEYFALDLIMEDGVCMGVTAWKLDDGTLHRFQAQKTVLATGGYGRAYLSATSAH
                     TCTGDGGGMALRAGLPLQDMEFVQFHPTGIFPAGCLITEGARGEGGYLTNSEGERFME
                     RYAPSVKDLAPRDMVSRAMTIEIREGRGVGPNKDHIFLHLDHLDPAVLHERLPGISET
                     AKVFAGVDVTRAPIPVLPTVHYNMGGLQTNYHGEVVTKTGDNADTIVPGLMAVGEAAC
                     VSVHGANRLGSNSLIDLVVFGRAVGMRCAEQLKPGATQPELKAGMTDGHLARFDRLRN
                     AAGPRPTADLRLEMQRAMQEDAAVFRTGESLKSGVERVNAVVAARKDIGVSDRGLVWN
                     SDLMETLEFDNLVCQAAVTVAGALNRTESRGAHAREDFPDRDDDQWMKHTLAWFDDAS
                     GKVVIDYRPVHSYTMSNDIAYIPPKARVY"
     gene            complement(2517..2918)
                     /gene="sdhD"
                     /locus_tag="PLO65_06025"
     CDS             complement(2517..2918)
                     /gene="sdhD"
                     /locus_tag="PLO65_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRD27838.1"
                     /translation="MTATGAGFRTPLSRARGLGSAKHGVGHFIADRVTGLALIPLSIW
                     AAFAGLKAAAGGYGAAVEWLAHPVNAVLLALFLVTGLVHLRLAIQVVIEDYIPGFVAK
                     SALLIANYLVCVLGAALGVFAILKVALSGAF"
     gene            complement(2915..3325)
                     /gene="sdhC"
                     /locus_tag="PLO65_06030"
     CDS             complement(2915..3325)
                     /gene="sdhC"
                     /locus_tag="PLO65_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRD27839.1"
                     /translation="MTEASSGVRERPLSPHLQVWRWHVTMLGSILHRACLIGLYVGAL
                     ILVGWAVALASGEAAYAAYVGLLGSPPGKLVLLGLTFALFFTAASSLRHLVWDTGRGF
                     TPKTADMITIAGIAFALVATAVVWVIAGQTGGLS"
     gene            complement(3401..4561)
                     /gene="zapE"
                     /locus_tag="PLO65_06035"
     CDS             complement(3401..4561)
                     /gene="zapE"
                     /locus_tag="PLO65_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRD27840.1"
                     /translation="MPLSVRAAYRERLSRGDIRPDAAQEQALVALSRLEGDLNALSEP
                     GFSSLFRKPKGARGLYLWGPVGRGKSMLMDLFFDSAPVAKKRRTHFHVFMAEVHAHID
                     AWRKGDAAARKARFGQSGPALGLAFGQARGGDDPIQPTAELIAEDARLLCFDELQVTD
                     IADAMILGRLFEALFAQGVTLVATSNRPPDDLYKDGLNRQLFLPFIAMLKERLEVVAV
                     RGPVDFRLDRLRGARTWLAPADPDNQREFDRLWTDMLDGAPEPGASLEVLGRRLHLPR
                     ASGGLLRASFASLCEHALGPQDYLAIADRFHTVFLEDVPRLTPARRDAAKRFNTLIDA
                     LYEAGAKLVALAAVEPEALYPEGDGSFEFERTVSRLQEMRSTDWLERVRDET"
     gene            4631..5995
                     /locus_tag="PLO65_06040"
     CDS             4631..5995
                     /locus_tag="PLO65_06040"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRD27841.1"
                     /translation="MKTIIEPFRIKAVEPIRMTTREERLKLIAEADHNLFALHSDDVL
                     IDLLTDSGTSAMSARQQAALMSGDESYAGSPSFFRFEAAVKQLMPFKHVIPTHQGRAA
                     EAILFSILGGPGRMIPSNTHFDTTRGNIESTGAEGHDLVIAEGLDPRSDHPFKGNMDL
                     DRLQAFLAEHGDAVPCVMLTVTNNAGGGQPVSLANIRAVARIAHAHRKPFIIDGCRFA
                     ENAYFIKMRETGQEGRSIKDIVGDMFAMADGMTMSAKKDAFANIGGWLALNDDDLAQA
                     ARNRLILTEGFPTYGGLAGRDLDAIAQGLTEIIDEDYLRYRLRSMAYIGERLEAMGVP
                     TVRPTGGHAVFIDARNMLPHIPPSQYPGQALAVAMYVEGGVRSCEIGTVMFGLRPDGT
                     EVEAPMDLVRMAFPRRVYTQSHADYIVEVFEEIVARKDSLRGMRIIWQPAAMRHFTAK
                     FAPL"
     gene            complement(6092..6724)
                     /locus_tag="PLO65_06045"
     CDS             complement(6092..6724)
                     /locus_tag="PLO65_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27842.1"
                     /translation="MKKLVVWFVLLAVVAVVAVVAYWQVDLRWRPRTITKHQAEIAKI
                     LDGAGWVSPGLTGPKLYMIGFRSCPNCIQAKAQLLPALQKAGVDTRVIMFARRDVNGL
                     AQSTPAERATVAELWLNRSWALMDRWDKAPIAAWTAEGLPPADGDMARTAVIDAGRGM
                     VDQLIPLLKDNGIGFGTPVLIWWSSDGVMHGCSCGSPASYRFVLKDLGAG"
     gene            6808..>7331
                     /gene="rsmI"
                     /locus_tag="PLO65_06050"
     CDS             6808..>7331
                     /gene="rsmI"
                     /locus_tag="PLO65_06050"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRD27843.1"
                     /translation="MSRHPPPPSLSEVPLEPGLYVVATPIGNLRDITLRALDVLAGCD
                     LLLAEDTRVTGKLLAAYGISKTMLRYDEHVAGRAWPRILAALAQGQSVALVSDAGTPL
                     VSDPGFRLVKDVIAAGHRVFPIPGPSAALAALSLAGLPTDRFLFAGFPPPKGGARRTF
                     LAEVADVHATLIFY"
BASE COUNT         1135 a         2517 c         2569 g         1110 t
ORIGIN      
        1 cccttggggc agacctgggc gcagttcatg atcgtgtggc agcgatagag cttgaacggg
       61 tcctcgagcg cgtcgagccg ctcgccggtg gcctcgtcgc ggctgtcgat cagccagcgg
      121 taggcgtgca gcagggtggc cggcccgagg tatttctcgc cgttccacca gtagctgggg
      181 caggaggtcg agcagcaggc gcagaggatg cactcgtaca gcccgtcgag cttcgaacga
      241 tcctcgaccg actgcttcca ctcggtgcgc ggctcggggg tctcggtgtg cagccacggc
      301 tcgatcgacg cgtactgggc gtagaacagg gtcaggtcgg gcaccaggtc cttgaccacc
      361 ggctggctgg gcagcggcga gatctggatt tccttgcccg ggacatcttc ccagccgtgc
      421 gtgcaggcca gggtgttgcg gccgccgatg ttcatcgcgc aggagccgca gacgccttcg
      481 cggcaggacc ggcggaaggt cagggtcggg tcgaccgtgt tcttgatgtg gatcagcacg
      541 tccagcagca tcgggccgca ggcgtcgacg tcgacgtcat aggtgtccca gcgcgggttc
      601 tccgaccctt ccggatcgta gcgatagatg cggaaggtct tgacccgttt ggccccctgc
      661 ggcgccgggt agtgacggcc cttgccgatg cgggagttct tgggaagcgc gagctgaacc
      721 atgccgtcct cagtaaaccc gcgccttggg cgggatgtag gcgatgtcat tcgacatcgt
      781 gtagctgtgc accggccggt aatcgatgac caccttgccg ctggcgtcgt cgaaccaggc
      841 aagggtgtgc ttcatccact ggtcgtcgtc gcggtcgggg aagtcctcgc gggcgtgggc
      901 gccgcggctc tcggtgcggt tcagggcccc ggcgacggtg acggcggcct ggcagacgag
      961 gttgtcgaac tccagcgtct ccatcaggtc gctgttccag accaggccac ggtcgctgac
     1021 accgatgtcc ttgcgggcgg cgaccacggc gttgacgcgc tcgactccgc tcttgagcga
     1081 ctcgccggtg cggaagacgg cggcgtcctc ctgcatggcg cgctgcatct ccagccgcag
     1141 gtcggccgtg ggacgcgggc cggcggcgtt gcggaggcgg tcgaagcggg ccaggtgacc
     1201 gtcggtcatg ccggccttga gttccggctg ggtcgccccc ggcttgagct gctcggcgca
     1261 gcgcatgccg accgcgcggc cgaacaccac caggtcgatc aggctgtttg aaccgaggcg
     1321 gttggccccg tgcaccgaca cgcaggcggc ctcgcccacg gccatcaggc ccggaacgat
     1381 ggtgtcggcg ttgtcgccgg tcttggtcac cacctcgccg tgatagttcg tctgcaggcc
     1441 gcccatgttg tagtggaccg tcggcaggac cgggatcggg gcccgggtca cgtcgacgcc
     1501 cgcgaacacc ttggccgtct cggaaatgcc cggcaggcgc tcgtgcagca cggcggggtc
     1561 gagatgatcc aggtgcagga agatgtggtc cttgttcggg cccacgccgc gaccctcgcg
     1621 gatctcgatg gtcatggcgc ggctgaccat gtcgcgcggg gccaggtcct tcacggacgg
     1681 cgcatagcgc tccatgaagc gctcgccctc ggaattggtc aggtagccgc cctcgccacg
     1741 ggcgccctcg gtgatcaggc agccggccgg gaagatgccg gtcgggtgga actgcacgaa
     1801 ctccatgtcc tgcagcggca ggccggcgcg cagggccatg ccgccgccat cgccggtgca
     1861 ggtgtgggcg ctggtcgccg acaggtaggc gcggccgtag ccgccggtgg ccagcacggt
     1921 cttctgggcc tggaaccggt gcagggtgcc gtcgtccagc ttccaggcgg tgacgcccat
     1981 gcagacgccg tcttccatga tcaggtcgag ggcgaagtac tcgatgaaga actcgacgtc
     2041 cttgctcagc gcctggccgt acatcgtgtg cagcatggcg tggccggtgc ggtcggccgc
     2101 cgcgcaggtc cgctggatcg gggcctcgcc gtagttgcgg gtcatgccgc caaaggcgcg
     2161 ctggtagatc ttgccttccg gcgtgcgcga gaagggcacg ccccagtgtt cgagctcgta
     2221 cacggcggcc ggggcgttgc gggtcaggta ttcgatggcg tcctggtcgc ccagccagtc
     2281 cgaccccttg acggtgtcgt acatgtgcca gcgccagtcg tcctcgccca tgttgcccag
     2341 cgacgccgag atgccgccct gggcggctac ggtgtgcgag cgggtcggga agaccttggt
     2401 tacgcaggcg gtcttcaggc cggcctgggc gcagccgacg gcggcgcgaa ggcccgagcc
     2461 cccggccccc accaccacga cgtcgaagcg gtggtcgatg aattcatagg ccggcatcag
     2521 aaggctccgg agagggccac cttgaggatg gcgaagaccc ccagagcggc gccgaggacg
     2581 cagacgagat agttggcgat gagcagcgcg gacttggcca cgaagcccgg gatgtagtcc
     2641 tcgatcacca cctggatggc gagccgcagg tggaccaggc cggtcaccag gaacagcgcg
     2701 agcagcacgg cgttgaccgg atgggccagc cattcgacgg cggccccgta gccgccggcg
     2761 gcggccttca gccccgcgaa ggcggcccag atcgacagcg ggatgagggc gaggccggtg
     2821 acccggtcgg cgatgaagtg acccacgccg tgcttggccg agccgaggcc gcgggcgcgt
     2881 gacagggggg tgcggaaacc ggcgccggta gcggtcacga caggcctccc gtctggccgg
     2941 cgatgaccca gaccaccgcc gtggcgacca gggcgaaggc gatgccggcg atggtgatca
     3001 tgtcggcggt cttcggcgtg aagccccggc cggtgtccca caccaggtgg cgcaggctgc
     3061 tcgcggcggt gaagaacagg gcgaaggtca ggcccagaag gaccagcttg ccgggcggcg
     3121 atcccagcag gccgacgtag gcggcgtagg ccgcctcacc cgacgccagg gccacagccc
     3181 accccacgag gatcaacgcg ccgacataaa ggccgatcag gcaggcgcgg tggagaatgg
     3241 agcccagcat ggtcacatgc cagcgccaca cctgcaggtg gggcgaaagg ggccgctcac
     3301 ggacccctga cgaggcttcg gtcatttggt tcccgacgat tgcttggctg cagggctatt
     3361 tacgccgcga acggggggtg tcaacgcgag caggggtttt tcaggtctcg tcccgcaccc
     3421 gctcgagcca gtcggtggag cgcatttcct gcaggcggga gacggtgcgc tcgaactcga
     3481 acgatccgtc gccctccgga tagagcgcct ccggctcgac ggcggccagg gcgaccagct
     3541 tggcccccgc ctcgtagagg gcgtcgatca gggtgttgaa ccgcttcgcc gcgtcccgcc
     3601 gggcgggcgt caggcgcggc acgtcctcga ggaacaccgt gtggaagcgg tcggcgatgg
     3661 ccagatagtc ctgcggcccc agggcgtgtt cgcacaggct ggcgaaactc gcccgcagca
     3721 ggccgccgct ggctctcggc agatgcagcc ggcggccgag aacctccagg ctggccccgg
     3781 gctcgggcgc gccgtcgagc atgtcggtcc acagccggtc gaattcccgc tggttgtccg
     3841 gatcggccgg ggccagccag gtccgggctc cccgcagccg gtcgagccgg aaatcaaccg
     3901 gcccccgcac ggccaccacc tccagccgct ccttgagcat ggcgatgaac ggcaggaaca
     3961 gctggcggtt caggccgtcc ttgtagagat cgtccggcgg ccggttggag gtggcgacca
     4021 gggtcacacc ctgggcgaac agcgcctcga acaaccggcc caggatcatc gcatcggcga
     4081 tgtcggtgac ctgcagttcg tcgaaacaga gcagacgcgc gtcctcggcg atcagctcgg
     4141 cggtcggctg gatcggatcg tcgccccccc gggcctggcc aaaggccagc ccgagggcag
     4201 gccccgactg gccgaagcgc gccttgcggg ccgccgcgtc gcccttgcgc caggcgtcga
     4261 tgtgggcgtg cacctcggcc atgaagacgt ggaagtgggt gcggcgcttc ttcgccaccg
     4321 gggcggagtc gaagaacagg tccatcagca tggacttgcc ccggccgacc ggtccccaga
     4381 gatagaggcc gcgcgcgccc ttcggcttgc ggaacagcga cgagaacccc ggctccgaca
     4441 aggcgttgag gtcgccctcg aggcgcgaca gcgcgaccag cgcctgctcc tgcgccgcgt
     4501 cgggcctgat gtcgccccgg gaaagacgct cgcggtaggc ggcgcggacg gataaaggca
     4561 tccccaaagc ggttagcgcg gcgcgccctc gccttcaatg ggtcgcgccc caacggatat
     4621 cgagtcaccc atgaaaacca tcattgaacc cttccgcatc aaggcggtcg aaccgatccg
     4681 catgacgacg cgcgaggaac ggctgaagct gatcgccgag gccgaccaca acctgttcgc
     4741 cctgcactcc gacgacgtgc tgatcgacct gctgaccgac agcgggacct cggccatgag
     4801 cgcccgccag caggcggcgc tcatgtcggg cgacgaaagc tacgccggca gcccgtcctt
     4861 cttccgcttc gaggcggcgg tgaagcagct gatgccgttc aaacatgtga tcccgaccca
     4921 ccagggccgg gcggccgagg cgatcctgtt ctcgatcctc ggcgggcccg gccggatgat
     4981 cccgtccaac acccatttcg acacgacgcg cggcaacatc gaatcgaccg gcgccgaagg
     5041 ccacgacctg gtcatcgccg aagggctcga tccgcgcagc gaccacccgt tcaagggcaa
     5101 catggacctc gaccggctgc aggccttcct ggccgagcac ggcgacgcgg ttccctgcgt
     5161 catgctgacg gtgaccaaca acgccggcgg cggccagccg gtcagcctgg ccaacatccg
     5221 cgccgtggcg cgcatcgccc atgcgcacag gaagccgttc atcatcgacg gctgccgctt
     5281 cgccgagaac gcctacttca tcaagatgcg cgagaccggc caggagggcc gctcgatcaa
     5341 ggacatcgtc ggcgacatgt tcgccatggc cgacggcatg accatgtcgg ccaagaagga
     5401 cgccttcgcc aacatcggcg gctggctggc gctgaacgac gacgacctgg cccaggccgc
     5461 gcgcaaccgg ctgatcctga ccgagggctt cccgacctac ggcggcctgg ccggccggga
     5521 tctcgacgcc atcgcccagg gcctgaccga gattatcgac gaggactacc tgcgctaccg
     5581 cctgcgcagc atggcctata tcggcgagcg gctggaggcg atgggggtgc cgaccgtccg
     5641 gccgaccggc ggccacgcgg tgttcatcga cgcccgcaac atgctgccgc acatcccgcc
     5701 gtcgcagtac cccggccagg cgctggcggt ggcgatgtac gtcgagggcg gcgtccgctc
     5761 ctgcgagatc ggcacggtga tgttcggcct gaggcccgac gggaccgagg tggaagcgcc
     5821 gatggacctg gtccggatgg ccttcccgcg ccgcgtctac acccagagcc acgccgacta
     5881 catcgttgag gtgttcgagg aaatcgtggc ccgcaaggac agcctgcgcg gcatgcgcat
     5941 catctggcag cccgccgcca tgcgccactt cacggcgaag ttcgcgccgc tgtagcacgg
     6001 gggtccgatt ggatgccccc caatcggccg ctcgagatct ttcagtgtcg cgcgcttgcc
     6061 gatcgccggt tcccgcgcat cgcaacgcgc tctagccggc gcccaggtcc ttcagcacga
     6121 agcggtagct cgccgggctg ccgcaggagc agccgtgcat cacgccgtcg ctcgaccacc
     6181 agatcagcac cggcgtgccg aagccgatgc cgttgtcctt cagcagggga atcaactggt
     6241 cgaccatgcc gcgccccgca tcgatcaccg cggtgcgcgc catgtcgccg tcggccgggg
     6301 gcaggccctc ggccgtccag gccgcgatcg gcgccttgtc ccagcggtcc atcagggccc
     6361 agctgcggtt gagccacagc tcggccaccg tcgcccgttc cgccggcgtc gactgggcca
     6421 ggccgttgac gtcgcggcgg gcgaacatga tcacccgcgt gtcgaccccg gccttctgta
     6481 gcgccggcag cagctgcgcc ttggcctgga tgcagttggg gcaggaccgg aagccgatca
     6541 tgtagagctt cggaccggtc agtccgggcg agacccagcc ggctccgtcg aggatcttcg
     6601 cgatctcggc ctgatgcttc gtgatggtcc tgggccgcca gcgcagatcg acctgccagt
     6661 aggcgaccac ggccacgacg gcgacgacgg cgagcagcac gaaccagacg acgagcttct
     6721 tcacggcgct tcctctcttg gttgccgtct tctatccctt gacggacgcg gtcgggcaag
     6781 gcgctctcgc gccgaccgga ggtcaccttg tcccgccacc cgccgccgcc gtcgctgtca
     6841 gaggttccgc tggagcccgg cctctatgtc gtggcgacgc cgatcggcaa cctgcgggac
     6901 atcaccctgc gggcgctgga cgtgctggcc ggctgcgacc tgctgctggc cgaggacacc
     6961 cgggtgaccg gcaagctgct ggccgcctac ggtatttcca agaccatgct gcgctatgac
     7021 gagcacgtcg ccgggcgcgc ctggccgaga atcctggccg cgctggcgca gggccagagc
     7081 gtggcgctgg tgtccgacgc cggcacgccg ctggtgtccg accccggctt ccggctggtg
     7141 aaggacgtca tcgccgccgg ccaccgcgtg ttcccgatcc ccggtccctc ggcggcgctg
     7201 gcggccctgt ccctggcggg cctgccgacg gaccggttcc tgttcgcggg cttcccgccg
     7261 ccgaagggcg gcgcgcggcg aaccttcctc gccgaggtgg ccgacgtcca cgccaccctg
     7321 atcttctacg a
//
LOCUS       DAORNX010000100         7319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7514_length_7319_cov_7.648678, whole genome shotgun sequence.
ACCESSION   DAORNX010000100 DAORNX010000000
VERSION     DAORNX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_7319_cov_7.648678"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLO65_06055"
     CDS             <1..885
                     /locus_tag="PLO65_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRD27844.1"
                     /translation="IALATAAPAAPLSPAQAALFQHGMLIAIIVLVGWISMVALDIAA
                     TLYMRGLRLDVEDNLLARKHVTQVRILRRVGATIIVLVTVAMALMTISGVRQWGVSLL
                     AAGGAAGIIVGLALQPLLTNLIAGLQIALTQPIRIDDAVIVEGEWGWVEEISATFVAV
                     RLWDLRRMVLPLTYFIQTPFQNWTRDSAALIGTVMLYVDYAAPVEAMRARLEAIVRST
                     PLWDGKVVALQVTDLRERVMEVRCLAGGRNAPQTFDLRCLVREQMIAFLKAEHPEALP
                     RDRLDLAAAAPSTDSRPG"
     gene            complement(882..1271)
                     /locus_tag="PLO65_06060"
     CDS             complement(882..1271)
                     /locus_tag="PLO65_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HRD27845.1"
                     /translation="MDQLTDALAASLELVADRCGDPTPLVYERLFAQQPGMRELFVGD
                     PAGAARGHMLQMVLDTLTGMADGEAWPANMIRAERINHDQIGVPFDVFATFFSVVHET
                     FREAGGEAWTPDMEAAWTGVLARAAGC"
     gene            complement(1259..2374)
                     /locus_tag="PLO65_06065"
     CDS             complement(1259..2374)
                     /locus_tag="PLO65_06065"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRD27846.1"
                     /translation="MPDDASKPAAKARTPPMKFGEGWLRDCWYFACQAHELKPGRLQR
                     FELLGEPVLLGRRRDGAVYALKDICPHRAAPLSAGKLVAETGGGESVECPYHGWRFGT
                     DGGCAAIPSLVESQDLDISRIRVRRYAAAESQGMVFVWFSSDPRFGMDSGDAPDSPPP
                     LFPGAVGRPAIVDHMDFDTHMDHAIVGLMDPTHAPYVHAAWWARSARRMKDKAKVFEP
                     SPEGFVMTRHATSSANRIYAILGGQPQTEITFRLPGYRWEHIQVGRFQVLALTCLTPI
                     TETKTRLTQLMWSDHPAMMLRWLIAPFMRKFIHQDGDMVNLQNMGLKYDPSLLWIDDA
                     DTQAKWYQQLKREWAASRAEGRPFVNPVKPATLRWIS"
     gene            complement(2554..4029)
                     /locus_tag="PLO65_06070"
     CDS             complement(2554..4029)
                     /locus_tag="PLO65_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HRD27847.1"
                     /translation="MKTWAKVALAGVGVAALLGGVVAVRTATFKPATQVDLSAVKIAA
                     ATPVDQAQAALHLSQAIRILTISHQNKAENDWAEWDRLHAWLATTYPAAHAAMTREIL
                     PNKALVYTWQGSDPSLAPIILMGHQDVVPVTAGTEADWKHPPFAGEIAEGAVWGRGSI
                     DDKGTLVMMFEAAEAMAAKGFKPKRTVIFVSGQDEEAGGTGAQAAAALLKSRGVKALF
                     VLDEGMAVVRDFPMVGRPVAVVGLGEKGYATLKIVAPATGGHSSAPPKATGVETLAKA
                     VLAVTSHPFPMKFEGPGADMIRAIAPYAGGVVKMAAANSWLFAPVLIGELSKSPASAA
                     TLHTTIAPTMLKGSPKENVLPQDATAWINYRIAPGQTAEEVMARAREVTRGLDVQLSW
                     EGGRAIDPSPVSSTTSDGWKILAALASDGGTIPVAPGLVTAGTDSRYMTGVAQDVYRF
                     APMHASLNEFGMIHGTNEHMSLENLKRMTDFYTRLIATAAG"
     gene            complement(4245..5810)
                     /locus_tag="PLO65_06075"
     CDS             complement(4245..5810)
                     /locus_tag="PLO65_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRD27848.1"
                     /translation="MSSPEQAPWWRGASVYQIYVRSFCDSNGDGQGDLPGVLAKLDYL
                     ADLGIDAIWLSPIHPSPNRDWGYDVADYEGVHPDYGTMADVEALLAAAHARGIRVILD
                     EVLAHTSDEHAWFRDSLKGGDRADWYVWADLKPDGTAPNNWLSAFGGPAWAYQPLRRK
                     AYHHKFLRQQPKLNWRNPDARAAALAVLDHWLTMGVDGFRLDVANAYLHDAALTDNPV
                     ETSDAGWRWSAAANFQKHWNDANLVENIEALADVRRTVERHADRFVFGEFSEEAGRCG
                     GFASAAEGLHAGYSFPLLHARKLGPDFIRRHFELLAEHPEHWPCVSFCNHDVPRTASR
                     FGDASPALAKLLLALLLALRGTVLLYQGEELGLPEADLTREQLRDPVGDLYWPAHKGR
                     DGCRTPMPWSSGRNLGFSNGTPWLPAAPEHAGLTVEAQAADDGSTLNFTRALLKARKA
                     SPALRLGEIRFLDLPAPLLGFDRIYRDERVRCLFNMSDAPVAAEAAGETLVQTGDIGG
                     GLFGPYAARIGAL"
     gene            5910..7022
                     /locus_tag="PLO65_06080"
     CDS             5910..7022
                     /locus_tag="PLO65_06080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD27849.1"
                     /translation="MSLLRRELLAALPAIAAAGCARAESTAPAGSLQRIVDETGVPAL
                     AGAVVTADGLPFLEAAGVRRMDAPGRVTGNDVWHLGSNTKAMTAALYGRLVEAGQARW
                     GATLPQLFPDLTPDPAWSATTIEQLMSHRAGATDHGAIDTAWLIASQKATNPVTEQRT
                     ALAAKVLAAPPAGRPGQFEYANLNFVLVGAAIERIVKTSWEAAITQRLFKPLGMTTAG
                     FGAPTGDQPRGHRRLPLGVGGLLPVDPSGISDNPPALGPAGTAHMSLTDYAKFIRLFL
                     TGGGDLLKPETIAHLTTPPAGADYALGWGVRTDRPWARGPVLAHEGSNTMWHMVALVA
                     PARGIAVLAASNAMMPDDRSAPRLLAKQLQERFAPA"
     gene            7067..>7319
                     /locus_tag="PLO65_06085"
     CDS             7067..>7319
                     /locus_tag="PLO65_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27850.1"
                     /translation="MTTTRLTGGSALPAIGLCLLAAMLEGFDIASMGVAAPKMIPALG
                     LSKPEAGWAFSASLLGLMIGAAVAGPLADQVFEPLLREGG"
BASE COUNT         1051 a         2542 c         2575 g         1151 t
ORIGIN      
        1 atcgccctgg ccaccgccgc gccggccgcg ccgttgtcgc cggcccaggc ggcgctgttc
       61 cagcatggca tgctgatcgc gatcatcgtt ctggtcggct ggatctcgat ggtggccctc
      121 gacatcgccg cgaccctcta catgcgcggg ctgcggctgg acgtggaaga caacctcctc
      181 gcccgcaagc atgtgaccca ggtgcgcatc cttcgccggg tgggcgcgac gatcatcgtc
      241 cttgtcaccg tggccatggc gctgatgacc atcagcggcg tgcggcagtg gggcgtcagc
      301 ctgctggcgg cgggcggcgc ggccgggatc atcgtcggcc tggcgctgca gccgctgctg
      361 accaacctga tcgccggcct gcagatcgcc ctgacccagc cgatccgcat cgacgatgcg
      421 gtcattgtcg aaggcgaatg gggctgggtc gaggagatca gcgccacctt cgtggccgtg
      481 cggctgtggg acctgcgccg gatggtgttg ccgctgacct acttcatcca gacgccgttc
      541 cagaactgga cccgcgattc tgcggccctg atcggaacgg tcatgctgta tgtcgactac
      601 gccgccccgg tcgaggcgat gcgggccagg ctggaagcga tcgtaaggtc gacgccgctg
      661 tgggacggca aggtggtcgc gctgcaggtg acggatttgc gcgagcgggt catggaagtc
      721 cgttgcctgg ccggcgggcg caatgcgcca cagaccttcg acctgcgctg tctggtgcgc
      781 gaacagatga tcgccttcct caaggccgag catcccgagg cgctgccacg cgatcggctg
      841 gacctcgccg cggccgcgcc gtccaccgac agccggcctg gctagcagcc cgccgcgcgc
      901 gccagcacgc cggtccacgc cgcttccatg tccggtgtcc aggcctcgcc gccggcttcg
      961 cgaaaggtct cgtggaccac ggagaagaag gtcgcgaaca cgtcgaacgg cacgccgatc
     1021 tggtcgtggt tgatccgctc ggcccggatc atgttggccg gccaggcctc gccgtcggcc
     1081 atgccggtca gggtgtcgag gaccatctgc agcatatggc cccgggccgc gcccgccggg
     1141 tcgccgacga acagttcccg catcccgggc tgctgggcga acagccgctc gtagaccagc
     1201 ggcgtgggat cgccgcagcg gtcggcgacc agttccaggc tggcggccag ggcgtcggtc
     1261 aactgatcca ccgcagtgtc gcgggcttga ccggattgac gaacggccgg ccctcggcgc
     1321 ggctggccgc ccattcccgc ttgagctgct ggtaccattt ggcctgggtg tcggcgtcgt
     1381 cgatccacag cagcgacggg tcgtacttca gccccatgtt ctgcaggttc accatgtcgc
     1441 cgtcctggtg gatgaacttg cgcatgaacg gggcgatcag ccaccgcagc atcatggccg
     1501 ggtggtccga ccacatcagc tgggtcagtc gcgttttcgt ctctgtgatc ggcgtcaggc
     1561 aggtcagggc cagcacctgg aagcggccga cctggatatg ctcccagcga tagccgggca
     1621 ggcggaaggt gatctcggtc tgcggctggc cgcccaggat ggcgtagatg cggttggccg
     1681 acgaggtcgc gtggcgggtc atcacgaacc cctccggcga gggttcgaac accttggcct
     1741 tgtccttcat ccgtcgggcc gaccgggccc accaggcggc gtggacatag ggcgcgtgcg
     1801 tcgggtccat cagcccgacg atggcgtggt ccatatgggt gtcgaaatcc atgtggtcga
     1861 cgatcgccgg tcggccgacg gcgccgggaa acaggggtgg agggctgtcg ggggcgtcgc
     1921 cgctgtccat gccgaagcgc gggtcggacg agaaccagac gaacaccatc ccctggctct
     1981 cggccgccgc atagcggcgc acgcggatcc ggctgatgtc gaggtcctgg ctctcgacca
     2041 gagacgggat cgccgcgcag ccgccgtccg tgccgaagcg ccagccgtga tacgggcatt
     2101 cgacgctctc gccgccgccc gtctcggcca cgagcttgcc cgccgacagc ggcgcggcgc
     2161 ggtgcgggca gatatccttc agcgcgtaga ccgccccgtc ccgccgccgg ccgagcagca
     2221 ccggctcgcc cagcagttcg aaccgctgca gcctgcccgg cttcagttca tgggcctggc
     2281 aggcgaaata ccagcagtct cgcagccagc cctcgccgaa cttcatcggc ggggtcctgg
     2341 ccttggcggc gggtttcgaa gcgtcgtcgg gcatggttct tcctacccct tctccccttg
     2401 cgggagaagg tggcccgcag ggcaggccgg atgaggggtc gatgcattga gccgcatcaa
     2461 ttcatgggca ggccaggtcg aaacagatcg cgcgacccct catccgtcag gctccgcctg
     2521 acaccttctc ccgcaagggg agaaggagcc atgctacccc gccgccgtcg cgatcagacg
     2581 ggtgtagaag tcggtcatcc gcttgaggtt ctccagcgac atgtgctcgt tggtgccgtg
     2641 gatcatgccg aactcgttca gcgaggcatg catcggggcg aagcgataga cgtcctgggc
     2701 cacgccggtc atgtagcggc tgtcggtgcc ggccgtgacc aggcccgggg cgaccgggat
     2761 cgtcccgccg tccgaggcca gggccgcgag gatcttccag ccgtcggagg tggtcgagga
     2821 caccggcgag gggtcgatcg cccggccgcc ttcccacgac agctgcacat ccaggccccg
     2881 ggtcacctcg cgggcccggg ccatcacctc ctcggcggtc tggccgggcg cgatgcggta
     2941 gttgatccag gcggtggcgt cctgcggcag cacgttctcc ttcggactgc ccttgagcat
     3001 ggtcggggcg atggtggtgt gcagcgtcgc ggcgctggcc gggctcttgg acagctcgcc
     3061 gatcagcacg ggcgcgaaca gccagctgtt ggccgcggcc atcttgacca cgccgccggc
     3121 atagggggcg atggcccgga tcatgtcggc gccgggaccc tcgaacttca tcggaaaggg
     3181 gtgggaggtg accgccagga ccgccttggc cagggtctcg accccggtcg ccttcggcgg
     3241 ggccgacgaa tggccgccgg tcgccggggc gacgatcttc agcgtcgcat agcccttctc
     3301 gccgaggccg acgaccgcca ccggccggcc gaccatcggg aagtcgcgca ccacggccat
     3361 gccctcgtcg agcacgaaca gcgccttgac tccgcgcgac ttgagcaggg ccgcggcggc
     3421 ctgggccccc gtgccgccgg cctcctcgtc ctggccgctg acgaagatca ccgtgcgttt
     3481 cggcttgaag cccttcgccg ccatcgcctc ggcggcctcg aacatcatca ccagggtgcc
     3541 cttgtcgtcg atcgagccgc ggccccagac cgcgccctcg gcgatctcgc cggcgaacgg
     3601 cgggtgcttc cagtcggctt cggtgccggc ggtcaccggc acgacgtcct ggtgacccat
     3661 gaggatgatt ggcgcgaggc tcgggtcaga gccctgccag gtgtagacca gcgccttgtt
     3721 gggcaggatc tcgcgggtca tggcggcgtg ggcggccgga taggtcgtcg ccagccaggc
     3781 atgcagccgg tcccattcgg cccagtcgtt ctcggccttg ttctggtggc tgatggtcag
     3841 gatccggatc gcctgcgaca ggtgcagggc ggcctgggcc tggtcgaccg gcgtcgcggc
     3901 cgcgatcttc acggccgaca gatcgacctg ggtggccggc ttgaaggtcg cggtgcgcac
     3961 cgccaccacc ccgccaagca acgccgccac gcccacgccc gccagcgcga ctttcgccca
     4021 ggtcttcatg cgatcctccc cgatccttgt tgcgggggag agtagagggg cgcccctgat
     4081 cccgccaacc cactttcctc tcccggaggg agagggggac cagccggagg ctggtggaga
     4141 gggatgcgcc ggtgtcggtg aaggatttca accaaggagc tcctccctct ccgacccggc
     4201 tccgccgggc cacctctccc tcggggagag gaaggatggc ggtgttagag ggcgccgatc
     4261 cgcgccgcgt agggcccgaa caggccgccg ccgatgtcgc cggtctggac cagcgtctcg
     4321 ccggccgcct cggccgcgac gggcgcatcg ctcatgttga acagacagcg cacccgctcg
     4381 tcgcggtaga tgcggtcaaa tcccagcagg ggcgccggca ggtcgagaaa ccggatctcg
     4441 cccagccgga gggccgggct ggccttgcgc gccttcaaca gggcgcgggt aaagttcagc
     4501 gttgaaccat catcggccgc ctgggcctcg accgtcagcc cggcatgctc cggcgcggcc
     4561 ggcagccagg gtgttccgtt ggagaaaccc aggttgcggc ccgaagacca cggcatcggc
     4621 gtcctgcagc cgtcgcggcc cttgtgcgcc ggccagtaga gatcgccgac cgggtcgcgc
     4681 agctgctcgc gggtcaggtc cgcctcgggc aggccgagct cctcgccctg gtagagcagc
     4741 accgtgcccc tgagcgccag cagcagggcc agcaacagct tcgccagcgc cggcgaggcg
     4801 tcgccgaacc ggctggccgt gcgcggcaca tcgtgattgc agaacgacac gcaaggccag
     4861 tgctccggat gctccgccag cagttcgaaa tgccggcgga tgaagtcggg acccagcttg
     4921 cgcgcgtgca gcagcgggaa gctgtagccg gcatgcaggc cctcggccgc gctcgcgaag
     4981 ccgccgcagc gcccggcctc ctcggagaac tcgccgaaca cgaagcggtc ggcatgccgc
     5041 tcgaccgtgc ggcgcacgtc cgccagggcc tcgatatttt ccaccagatt ggcgtcgttc
     5101 cagtgcttct ggaagttcgc cgccgcgctc cagcgccagc cggcgtcgct ggtctcgacc
     5161 ggattgtcgg tcagggccgc gtcgtggaga taggcgttgg cgacgtcgag ccggaagccg
     5221 tcgacgccca tggtcagcca gtggtcgagc acggccaggg ctgcggcccg ggcgtcgggg
     5281 ttgcgccagt tgagctttgg ctgctggcgc aggaacttgt ggtgataggc cttgcggcgc
     5341 agcggctggt aggcccaggc cggcccgccg aaggcgctga gccagttgtt cggcgccgtc
     5401 ccgtccggtt tcagatcggc ccagacgtac cagtcggccc tgtcgccgcc cttgaggctg
     5461 tcgcggaacc aggcgtgttc gtccgaggta tgggccagca cctcgtcgag gatcacccgg
     5521 atgccgcgcg cgtgcgccgc cgccagcaac gcctcgacgt cggccatcgt cccgtagtcg
     5581 ggatggacgc cctcatagtc cgccacgtca tagccccagt cgcggttcgg cgacggatgg
     5641 atcggcgaca gccagatcgc gtcgatcccg aggtcggcca gatagtcgag cttcgccagc
     5701 acgcccggca ggtcgccctg gccgtcgccg ttggagtcgc agaagctgcg gacgtagatc
     5761 tggtagacgc tcgcgccgcg ccaccagggc gcctgttcgg gactgctcat cgggcgagcc
     5821 tagcggaccg gcgcgggttg cggaaacgtc acttgcctcc ggcggcggtc gttgttcgat
     5881 agcagcccag ggtctggtga ggatcaacga tgagccttct tcgccgcgaa cttctggccg
     5941 cccttccggc gatcgcggcc gccggctgcg ccagggccga gtcgacggcg ccggcagggt
     6001 cgctgcagcg gatcgtcgac gagaccggcg ttccagcgct ggccggcgcg gtggtcaccg
     6061 ccgacggcct gccgttcctc gaagcggccg gcgttcggcg gatggatgcg cccggtcgcg
     6121 tgaccggcaa tgacgtctgg catctcgggt ccaacaccaa ggccatgaca gcggccctct
     6181 atggacggct ggtcgaggcg ggtcaggcgc ggtggggcgc gaccctgccg cagctgtttc
     6241 cggatctgac gcctgatccg gcctggtcgg ccacgaccat cgaacagctg atgagccatc
     6301 gcgcgggcgc gaccgatcac ggcgccatcg acacggcctg gctgatcgcg tcgcagaagg
     6361 cgaccaatcc ggtgaccgag cagcggacag ccctcgcggc gaaggtcctc gcggccccgc
     6421 cggcgggcag gcccgggcag ttcgaatacg ccaacctgaa cttcgtcctc gtcggcgcgg
     6481 cgatcgagcg gatcgtgaag accagctggg aggcggcgat cacgcaacgg ctgttcaagc
     6541 cgctgggcat gaccaccgcc ggcttcggcg caccgacagg cgaccagccc cgggggcacc
     6601 gccggctgcc gctgggcgtt ggcggcctgc tgcccgtcga cccgtccggg atctccgaca
     6661 acccgcccgc cctgggtccg gccgggaccg cgcatatgtc gctgaccgac tatgcgaagt
     6721 tcatccgcct gttcctgacc ggaggcggcg atctgctgaa gccggagacc atcgcccacc
     6781 tgacgacgcc gccggccggc gccgactatg cgctgggctg gggcgtgcgc accgaccgcc
     6841 catgggcgcg cggacctgtc ctcgcccacg agggctccaa caccatgtgg cacatggtgg
     6901 ctctcgtcgc cccggcgcgc ggcatcgccg ttctggcggc gtccaacgcc atgatgcccg
     6961 atgaccgcag cgcgccgcgc ttgctggcga aacagctgca ggaacgcttc gcgccggcct
     7021 gattctgttc caggctcacc tgtctgtcac gctctggggg agcgccatga ccaccacccg
     7081 tctgaccgga ggatcggccc tgccggccat cggcctgtgc ctgctggcgg cgatgctcga
     7141 gggattcgac atcgcctcga tgggcgtggc cgcgccgaag atgatcccgg cgctgggcct
     7201 gtcgaaaccc gaggccggct gggccttcag cgccagtctt ctgggcctga tgatcggggc
     7261 ggccgtggcc gggccgctgg ccgaccaggt gtttgagccg ctgctgcgcg aaggcggag
//
LOCUS       DAORNX010000101         7286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7565_length_7286_cov_8.150602, whole genome shotgun sequence.
ACCESSION   DAORNX010000101 DAORNX010000000
VERSION     DAORNX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7565_length_7286_cov_8.150602"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLO65_06090"
     CDS             complement(<1..409)
                     /locus_tag="PLO65_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRD27851.1"
                     /translation="MTTAYDYDFTAIDGSALPMTTFRDKVVLVVNVASKCGLTPQYDG
                     LEQLYRDHRDQGLVVLGVPCNQFAGQEPGTEGEIAEFCRTNFGVDFPMTGKVDVKGDD
                     AHPFYKWAVSQVGEQINGALAERGGGAPAGGFMK"
     gene            complement(529..1167)
                     /locus_tag="PLO65_06095"
     CDS             complement(529..1167)
                     /locus_tag="PLO65_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27852.1"
                     /translation="MSGLTIWTYDWAPEGPRGFVRDLRLRWACEEAGLDYRVQTIPFE
                     GRETNHLDRQPFGQVPFLTDGDLEVFESGAGLLHLARKSDRLMPQDPAGEAATLQWTV
                     AALNSVEMVTVPWWFLNICGDEVNGLTGWMSQRLDHLERVLGEREWLAADRFTVADLL
                     MADVLRVPKVRAFGDRPATEAYVARITARPAFRKAHADQMALFEAADAGRAG"
     gene            complement(1273..2172)
                     /locus_tag="PLO65_06100"
     CDS             complement(1273..2172)
                     /locus_tag="PLO65_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD27853.1"
                     /translation="MEKVLKAGVVGSGVFGGFHARKYAEMDGVELAGIFDIHPERCFA
                     MAEQHGVEAWGDLDDLLAVCDIVTVASPATTHAAAALACLRAGKPVYVEKPLATTLDD
                     ADSLIAEASKRGLVLACGHQERVVSRAMGLLDIPEKPLLLEAVRKGTPSIRSQDVSVV
                     LDLMIHDIDLALSLTDAEPLAVEAEGDGDAIRAEATFGDGFTAIFTAARNATARERTM
                     RIVYPSGVLEIDFVTRAFSNGTPFPLNEAFTETPAGRDPLGTSVAGFVAAVRGAAPRP
                     FVTGEEAARALDLALAVEQAAGF"
     gene            2266..3267
                     /locus_tag="PLO65_06105"
     CDS             2266..3267
                     /locus_tag="PLO65_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD27854.1"
                     /translation="MTVSREIRLKSRPVGTPTADNFELATVELPAPGPGEVQVRNSWM
                     TVDPYMRGRMNDVKSYSPPFQLGEAMQGGAVGEVIASNDPSLKVGDLVQSMFGWREAF
                     NAPAGAVQKLNTFGLPPQTFLGVAGMPGMTAWVGLLKIAALKDGDVVFVSGAAGAVGS
                     VVCQIARIKGHKVIGSAGGADKVAFLKSIGCDEVIDYKAETDLTAALMRAAPTGIDVY
                     FENVGGAHMDAALAAARPFARFALCGMISQYNATGEMYGVKNLIMAVGKSLKLEGFIV
                     SNHFEHLPQFLQDMSGWISDGKLKWTETVDEGIENAPGAFLKLFKGENTGKMLVKLG"
     gene            complement(3365..3712)
                     /locus_tag="PLO65_06110"
     CDS             complement(3365..3712)
                     /locus_tag="PLO65_06110"
                     /inference="COORDINATES: protein motif:HMM:NF023036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HRD27855.1"
                     /translation="MNKFAVVVSVAALALSACGQKPAADGDLKSAKARALIIPPGPTG
                     PVLSTTGTVVAVDGRTVTLDHEAVEGLAAGRTSFRTWADVIAGSPGEPGARVTAKIQK
                     LGDGWAVVEMTGR"
     gene            3831..4739
                     /locus_tag="PLO65_06115"
     CDS             3831..4739
                     /locus_tag="PLO65_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HRD27856.1"
                     /translation="MSRLFSAYVMVDWSAAAAPRTGKDSIWIGVIKRDIRFRPTFEAF
                     NPATRDEAMKMLGEVLADLRRRNERALVGFDFPLGYPEGTAKALALKSPDWSGLWAFL
                     AANVVDKASNVNNRFAVAAKMNRLMTDQAWPFWGCPANDAQRWLATTKPTALPDGLPP
                     QLRRAEKLTQGKGKAGAKTVWQVFGNGTVGSQAIVGIPRVKALADQLGDRGRVWPFQT
                     GWKTLTPADVDGLEAVFAEVYPPLAEPKPEPGEVPDRAQVRALCEHFLRLDEAGDLAA
                     AFGPKAAVSDDERAVVEGEEGWILGV"
     gene            4810..6048
                     /locus_tag="PLO65_06120"
     CDS             4810..6048
                     /locus_tag="PLO65_06120"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRD27857.1"
                     /translation="MTTATERYQSIDAVRGFAVLGILLMNIVGMGLPTFAYISPTYWG
                     GHEGANLWAWAINFVLTDGKMRGLFTMLFGASMLLIADRAEGRRPGPAATHYRRVWWL
                     FVIGMIHAYFLFFGDILVTYAIAGALIFPLRRLGPKALIGLGAAVLVALLAKDFAEIS
                     YLKAIQAAMAAGTTDASQISLWNEVQLLLNAPRAMGEEEVRLMRGGFLEALQARGQAA
                     WIVQSQFMPVEEIPEAVGQMLIGAGLFRTGFFTLGWSSRAYGALIAIGYLVGAPLTAW
                     LAWSMVRVDFEPVQRHWFEVLGAGPRPFIALAHASLLMLVVRAGALKGLMNRLEAAGR
                     MAFSNYLMTSIITSFVFCGYGLGLYGQLERAELYWVVLGVWAFILLWSRPWLARFHYG
                     PFEWLWRSLVMWKRQPFVKR"
     gene            complement(6133..>7101)
                     /locus_tag="PLO65_06125"
     CDS             complement(6133..>7101)
                     /locus_tag="PLO65_06125"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRD27858.1"
                     /translation="DNINGMEFTAQERTPDPERLLKAYAQSSATLNLLRAFAGGGYAD
                     LHNIHRWTLGFVADSPQGARYRELSDKISESLDFMAAIGVTPESHPGMKQVEFFTSHE
                     ALLLGFEEAMTRVDSTSGDWYDTSAHMLWIGERTRQLDGAHIEFARGVKNPIGMKVGP
                     TLEPDDLLRLIDTLNPANEPGRLTLIGRFGADRIAERLPRLMAATKKAGRSVVWATDP
                     MHGNTMKANTGYKTRPFDRILAEVKGFVDVAEAEGVHPGGVHLEMTGQNVTECTGGAR
                     GVLEGELGDRYHTHCDPRLNGEQALELAFLVAERLKTARDDLREAS"
     assembly_gap    7102..7111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7112..>7286)
                     /locus_tag="PLO65_06130"
     CDS             complement(<7112..>7286)
                     /locus_tag="PLO65_06130"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRD27859.1"
                     /translation="EFHADNIRDTFRLILQMAVVLTFAGGKPVVKVGRIAGQFAKPRS
                     EPIETIGGVTLPS"
BASE COUNT         1099 a         2504 c         2508 g         1165 t
ORIGIN      
        1 tcttcatgaa gccaccggct ggagcgccac caccccgctc ggccaaagcg ccgttgatct
       61 gctcgccgac ctgcgagacg gcccacttgt agaacgggtg ggcgtcgtcg cccttcacgt
      121 cgaccttgcc ggtcatcggg aaatcgacgc cgaagttggt gcggcagaac tcggcgatct
      181 cgccttcggt ccccggctcc tggccggcga actggttgca gggcacgccc agcaccacca
      241 gcccctggtc gcgatggtcg cgatagagtt gctccaggcc gtcatactgc ggggtcaggc
      301 cgcacttgct ggccacattg accaccagca ccaccttgtc ccggaaggtc gtcatcggca
      361 gggcagagcc gtcgatggcg gtgaagtcgt agtcgtaggc ggtggtcatg caatcagctc
      421 cgtggcgggc ggactatggc cgcccggcgg acgaaattcc aagtcatctt gttgcgaacg
      481 ccggtgcagg tcgccggagc cgcaccagga tgttggcggg cccgcccttc aacccgccct
      541 gccagcgtcc gccgcctcga acagcgccat ctggtcggcg tgggccttcc ggaaggcggg
      601 gcgggcggtg atccgggcga cataggcctc ggtcgccggc ctgtcgccga aggcgcggac
      661 cttcggaacg cgcagcacgt ccgccatcag cagatccgcc acagtgaacc ggtccgccgc
      721 gagccattcc cgctcaccca gcaccctctc gaggtgatcg aggcgctggc tcatccatcc
      781 tgtaagacca ttgacctcgt cgccgcagat gttcaggaac caccagggga cggtgaccat
      841 ctcgaccgaa ttgagcgccg caacggtcca ttgcagcgtc gccgcctccc ccgccggatc
      901 ctgcggcatc agcctgtcgc tcttgcgcgc cagatgcagc agcccggccc cgctttcgaa
      961 gacctccagg tccccgtcgg tcaggaaggg aacctggccg aacggttgac ggtcaagatg
     1021 attggtctcg cggccctcga acgggattgt ctggaccctg tagtcgaggc cggcctcctc
     1081 gcaggcccag cgcaacctga gatcccgcac gaaccctcgg ggtccctcgg gggcccagtc
     1141 gtaggtccag atggtcagtc cgctcatcgc cgagaacctt tccttgtcga gcggcccgat
     1201 ccgccggtgg gcctggtcaa acgccggggc ggtcgcccgc ggggcgaaag ccctgacagg
     1261 ttccgacggt cgtcagaagc ccgccgcctg ctccaccgcc agcgcgaggt ccagcgccct
     1321 cgccgcttcc tcgccggtga cgaacggcct cggcgccgcc ccccgcaccg cggcgacgaa
     1381 gccggcgacg ctggttccca gcgggtcgcg accagccgga gtctcggtga aggcctcgtt
     1441 caacggaaac ggggtgccgt tggagaaggc gcgggtgacg aagtcgatct ccagcacgcc
     1501 ggacggatag acgatgcgca tggtccgctc gcgggccgtg gcgttgcggg cggccgtgaa
     1561 aatggcggtg aagccgtcgc cgaaggtcgc ctcggcccgg atcgcgtccc cgtcgccctc
     1621 ggcctcaacg gccaggggct cggcgtcggt cagcgacagg gccagatcga tgtcatggat
     1681 catcaggtcg agcaccaccg agacgtcctg gctgcggatc gacggcgtgc ccttgcgcac
     1741 ggcctccagc agcaggggct tctcgggaat gtcgagcaga cccatcgccc gcgagacgac
     1801 ccgctcctga tggccgcagg ccagcaccag gccgcgcttg ctcgcctcgg cgatgaggct
     1861 gtccgcgtca tcgagcgtcg tcgccagggg tttctcgacg tagacgggct tgccggcccg
     1921 gagacaggcc agggcggcgg cggcgtgggt ggtggccggc gaggcgaccg tgacgatgtc
     1981 gcagacagcc agcaggtcgt cgaggtcgcc ccaggcctcg accccgtgct gctcggccat
     2041 cgcgaagcag cgctcgggat gaatgtcgaa gatgccggcc agttcgaccc cgtccatctc
     2101 cgcgtatttg cgcgcgtgga agccgccgaa cacgcccgac ccgaccactc cggccttgag
     2161 taccttttcc atgaggcggt tctagcgcgc ttcgacgcct tccgcgaggt gaggaaacgg
     2221 gctaaacgcc tgtttgaaat tacaccgtaa agcgggagga accccatgac cgtgtcgcgc
     2281 gagatccgtc tgaagagtcg ccctgtcggg acccccacgg ccgacaactt cgaactggcc
     2341 acggtggagc tgccggcgcc cggccccggc gaggtccagg tgcgcaacag ctggatgacc
     2401 gtcgatccct acatgcgcgg ccgcatgaac gacgtgaaga gctacagccc gccgttccag
     2461 ctgggtgaag ccatgcaggg cggcgcggtg ggcgaggtga tcgcgtccaa tgacccgtcc
     2521 ctcaaggtcg gcgacctggt ccagagcatg ttcggctggc gcgaggcctt caacgccccg
     2581 gccggcgcgg tgcagaagct gaacaccttc ggcctgccgc cgcagacctt cctgggtgtc
     2641 gccggcatgc ccggcatgac ggcgtgggtc ggcctgctga agatcgccgc cctgaaggac
     2701 ggcgacgtgg tgttcgtgtc cggcgccgcg ggcgcggtgg gctcggtcgt ctgccagatc
     2761 gccaggatca agggccacaa ggtcatcggc tcggccggcg gggccgacaa ggtcgccttc
     2821 ctcaagtcga tcggctgcga cgaggtgatc gactacaagg ccgagacgga cctgaccgcg
     2881 gccctgatgc gcgcggcccc gaccggtatc gacgtctatt tcgagaacgt cggcggggcc
     2941 cacatggacg cggcccttgc cgccgcccgt ccgttcgccc gcttcgcgct gtgcggcatg
     3001 atcagccagt acaacgcgac cggcgagatg tacggcgtga agaacctgat catggccgtg
     3061 ggcaagagcc tgaagcttga gggcttcatc gtctccaacc acttcgagca cctgccgcag
     3121 ttcctgcagg acatgtccgg ctggatcagc gacggcaagc tgaagtggac cgaaacggtc
     3181 gacgagggca tcgagaacgc gcccggcgcc ttcctcaagc tgttcaaggg cgagaacacc
     3241 ggcaagatgc tggtgaagct gggctgacgg ttcgggtgca cggggacatg taccgccttc
     3301 ggtacgtgtc ccctgcagct tcaagctgat ggggacacgc actgaagtgc atgtccccgg
     3361 gctgtcaccg cccggtcatc tccaccaccg cccagccgtc gccgagcttc tggatcttcg
     3421 ccgtgacgcg ggcgccgggt tcaccgggcg aaccggcgat cacgtcggcc caggtgcgga
     3481 agctcgtgcg gccggcggcg aggccctcga cggcttcatg gtccagggtg accgtccggc
     3541 cgtcgacggc gaccacggtg ccggtggtcg acaacaccgg gccggtcggt cccggcggga
     3601 tgatcagggc gcgcgccttg gcggacttga ggtcgccgtc ggcggcgggc ttctggccac
     3661 aggccgacag ggcgagcgcg gcgaccgaaa cgacaacggc gaacttgttc atgaccctgc
     3721 tccgtggacg gaccgtccga ttatcacgaa cgcggggaat ttgaagcccg cgcttcctcc
     3781 cgggggcccc gaccgctaaa agcccggcca atccttggag gagtgagccc gtgtcgcgcc
     3841 tgttcagcgc ctatgtgatg gtcgactgga gcgccgccgc cgcgccgcgg acgggcaagg
     3901 actccatctg gatcggcgtc atcaagcgcg atatccgctt ccgcccgacg ttcgaggcct
     3961 tcaacccggc gacccgcgac gaggctatga agatgctcgg cgaggtgctc gccgacctgc
     4021 gccgccgcaa tgaacgggcg ctggtcgggt tcgacttccc gctcggctac ccggaaggca
     4081 cggcgaaggc cctggccctg aagtcgccgg actggagcgg cctgtgggcc ttcctggccg
     4141 ccaacgtcgt cgacaaggcg agcaacgtga acaaccgctt cgccgtggcc gccaagatga
     4201 accggctgat gaccgaccag gcctggccgt tctggggctg cccggccaac gacgcgcagc
     4261 gctggctggc gacgaccaag cccaccgccc tccccgacgg cctgccgccg caactgcggc
     4321 gcgccgagaa actgacccag ggcaagggca aggccggggc caagaccgtc tggcaggtgt
     4381 tcggcaacgg cacggtcggc agccaggcca tcgtcggcat cccgcgggtc aaggcgctgg
     4441 ccgaccagct gggtgaccgg ggcagggtct ggccgttcca gaccggctgg aagaccctga
     4501 cgccggccga tgtcgacggg ctggaggcgg tcttcgccga ggtctatccg ccgctggccg
     4561 aaccaaagcc cgaaccgggc gaggtgcccg accgggccca ggtgcgcgcc ctgtgcgagc
     4621 atttcctccg actcgacgag gccggcgacc tggcggccgc cttcggcccg aaggccgccg
     4681 tcagcgacga cgagcgggcg gtggtcgagg gcgaagaggg ctggatcctc ggggtctgac
     4741 cttccggcgg attgacttcc gccgaccgct gttcaccctc cctctcacta tcgccggggg
     4801 aggcgcgccg tgaccaccgc taccgaacgc taccagtcga tcgacgccgt gcgcggcttc
     4861 gccgtgctgg gcatcctgct gatgaacatc gtcggcatgg gcctgccgac cttcgcctat
     4921 atcagcccga cctactgggg cggccacgag ggggccaacc tgtgggcctg ggcgatcaac
     4981 ttcgtcctga ccgacggcaa gatgcgcggc ctgttcacca tgctgttcgg ggccagcatg
     5041 ctgctgatcg ccgaccgggc cgaggggcgc aggccagggc ccgcggcgac ccactaccgc
     5101 cgggtctggt ggctgttcgt catcggcatg atccacgcct acttcctgtt cttcggcgac
     5161 atcctggtga cctacgccat cgccggggcg ctgatcttcc cgctgcggcg gctcgggccg
     5221 aaggcgctga tcggcctcgg cgcggccgtg ctggtcgcgc tgctggccaa ggatttcgcc
     5281 gagatcagct acctcaaggc catccaggcg gcgatggccg cgggcacgac cgacgcctcc
     5341 cagatctcgc tgtggaacga ggtgcagctg ctgctcaacg cgccgcgcgc catgggcgag
     5401 gaggaggtcc ggctgatgcg cggcggcttc ctcgaggcgc tgcaggcccg cggccaggcc
     5461 gcctggatcg tccagagcca gttcatgccg gtcgaggaga tccccgaggc ggtcggccag
     5521 atgctgatcg gcgccgggct gttccggacc gggttcttca ccctgggctg gtccagccgg
     5581 gcctatggcg cgctgatcgc gatcggctat ctggtcgggg cgccgctgac cgcctggctg
     5641 gcctggtcga tggtgcgggt cgacttcgag ccggtgcagc ggcactggtt cgaggtgctc
     5701 ggcgcggggc cccggccgtt catcgccctg gcccacgcca gcctgctgat gctggtcgtg
     5761 cgggcgggcg cgctgaaggg gctgatgaac cggctggagg ccgccggccg gatggccttc
     5821 agcaactacc tgatgacctc gatcatcacc tcgttcgtgt tctgcggcta cggcctgggg
     5881 ctctatggcc agctcgagcg ggcggagctg tactgggtgg tgctcggcgt gtgggccttc
     5941 atcctgctgt ggagcaggcc ctggctggcg cggttccact acggcccgtt cgagtggctg
     6001 tggcgctcgc tggtgatgtg gaagcgccag ccgttcgtga aacgataggg gacatgtacc
     6061 gcaggtacgt gtcccccaac cccgggatca gactttaggg gacacgtacc ttcggtacat
     6121 gtccccgcct gatcagctcg cctcgcgcag gtcgtcgcgc gccgtcttca gccgttcggc
     6181 caccaggaag gccagctcca gcgcctgctc gccgttcagg cgcgggtcgc agtgggtgtg
     6241 gtagcggtcg ccgagctcgc cctcgagcac gccgcgcgcg ccgcccgtgc attcggtgac
     6301 gttctggccg gtcatctcca ggtgcacgcc gccgggatgg acgccctcgg cctcggccac
     6361 gtcgacgaag cccttcacct cggcgaggat ccggtcgaac ggccgggtct tgtagccggt
     6421 gttggccttc atcgtgttgc cgtgcatcgg gtcggtggcc cagaccaccg agcggccggc
     6481 cttcttcgtc gccgccatca gccgcggcag ccgctcggcg atcctgtcgg cgccgaagcg
     6541 gccgatcaac gtcagccggc ccggctcgtt ggccggattg agcgtatcga tcagccgcag
     6601 caggtcgtcc ggctcgagcg tcgggccgac cttcatgccg atcgggttct tcaccccgcg
     6661 cgcgaactcg atgtgcgccc cgtccagctg ccgcgtgcgc tcgccgatcc acagcatgtg
     6721 ggccgaggtg tcgtaccagt cgcccgaggt cgaatcgacg cgcgtcatag cctcctcgaa
     6781 gcccagcagc agagcctcgt ggctggtgaa gaactccacc tgcttcatgc ccggatggct
     6841 ctcgggcgtg accccgatgg cggccatgaa gtcgaggctc tcgctgatct tgtccgacag
     6901 ctcgcggtag cgcgcgccct gcgggctgtc ggccacgaag cccagggtcc agcggtggat
     6961 gttgtgcaga tcggcgtagc cgccgccggc gaaggcccgc agcaggttca gcgtcgccga
     7021 cgactgggcg taggccttca gcagccgctc gggatcgggc gtgcgctcct gcgccgtgaa
     7081 ctccatgccg ttgatgttgt cnnnnnnnnn ntaggacggc agggtcaccc cgccgatggt
     7141 ctcgatcggc tcggagcgcg gcttggcgaa ctggccggcg atacggccga ccttcaccac
     7201 cggcttgccg ccggcgaagg tcagcaccac ggccatctgc aggatcaggc ggaaggtgtc
     7261 gcggatgttg tcggcgtgga attcct
//
LOCUS       DAORNX010000102         7265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7595_length_7265_cov_7.618585, whole genome shotgun sequence.
ACCESSION   DAORNX010000102 DAORNX010000000
VERSION     DAORNX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_7265_cov_7.618585"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1782
                     /locus_tag="PLO65_06135"
     CDS             202..1782
                     /locus_tag="PLO65_06135"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRD27860.1"
                     /translation="MELGVVELSRLQFALTAMYHFLFVPLTLGLSFLLVIMESIYVMT
                     GRPIWRTITRFWGTLFGINFVLGVATGLTMEFEFGTNWSYYSHYVGDVFGAPLAIEGL
                     MAFFLEATFVGLMFFGWERLSRRGHLLVTFMVALGSNLSALWILIANGWMQNPVGAVF
                     NPDTMRMEVSSFRDVLFNPVAQAKFVHTVSAGYVTAAAFVLGVSALMLLRGRWPRVAK
                     RSFAVAAAFGLASALSVVVLGDESGYALTDNQKMKLAALEAMWETEPAPAGLTAFGVP
                     DMASRTTKAEIKIPYVLGLIATRSLDKPVIGILPLVAIAETRIENGVKAYDALERLKA
                     APTDPAARADFELARGDLGYGLLLKRYVADPRMADRATIQRAAWDTVPNVPVMFWAFR
                     AMAGIGFLMIAVFGAAFVMVSLRRSQARWLLWLAVVVMPLPWIAAEAGWVLAEMGRQP
                     WAVEGVLPTFLGTSSLTAGQIWTTIIGFTVIYSALAVVEVGLMVRTIRRGPYAHHEDP
                     DQAEAVAPVADPAAEGAR"
     gene            1782..>2595
                     /gene="cydB"
                     /locus_tag="PLO65_06140"
     CDS             1782..>2595
                     /gene="cydB"
                     /locus_tag="PLO65_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRD27861.1"
                     /translation="MDIPIDYPTLRLIWWGLMGVLLIGFALTDGYDLGVTALLPFVAR
                     DDGERRMVINTVSAHWEGHQVWFILGGGAIFAAWPFVYAVSFSGFYLAMFAVLAALIL
                     RPVGFKYRSKRPSPLWRGSWDWALFVGGFAPALVFGVAVGNVLQGLPFRLDGDLRAFY
                     EGTFLGLFTPFTLVCGLLSVAMLVTHGAAWLAIRAEPGPVRDRARRFGAIAAAAVIVL
                     FAAGYAFVAFGGFGFRLDGPVDPAGPSNPLRAGAVAAAGGWLTNFRIWPWMWL"
     assembly_gap    2596..2605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2606..2937
                     /locus_tag="PLO65_06145"
     CDS             <2606..2937
                     /locus_tag="PLO65_06145"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRD27862.1"
                     /translation="ILGFGGAILALIGIRGGREALAFGGSSLAVLGGISTVGLSMFPF
                     ILPSSLDPGSSLTAWNASSSHMTLFVMLIVTVVFLPMVLAYTAWALRVMWGRSTIDAL
                     STNPDLY"
     gene            2952..3071
                     /gene="cydX"
                     /locus_tag="PLO65_06150"
     CDS             2952..3071
                     /gene="cydX"
                     /locus_tag="PLO65_06150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HRD27863.1"
                     /translation="MWYFTWVLGLGLAVAFGILNGMWHEFDLMESGDLGEDDV"
     gene            complement(3085..3417)
                     /locus_tag="PLO65_06155"
     CDS             complement(3085..3417)
                     /locus_tag="PLO65_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD27864.1"
                     /translation="MSTVLAAGPTSREALDLADSAHSAARLLKMMASEQRLLLLCRLV
                     DGEASVGDLATHVGLAQSATSQHLAKMRAEGLVATRRDAQTIYYRLADPAAMRVLDTL
                     CEIYRAQT"
     gene            complement(3506..6712)
                     /locus_tag="PLO65_06160"
     CDS             complement(3506..6712)
                     /locus_tag="PLO65_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD27865.1"
                     /translation="MNLGLSGRLTRATIRSPLTPLFLLAAIAVGLLALVSIPREEEPQ
                     ISVPMVDIMVAAPGLRAPDAVELVGKPLETIVKSIHDVEHVYTFADDDQVMVTARFKV
                     GVDPDAAAVRIHERIQANLDRIPAGIAEPLVQTRGINDVPSLVLTLSPKPGAAGQWSD
                     QALYELAGKLRTEVAKVDDVGLTFIVGGRPQEIRVEPDPARLAMHGVSLGGLMETIRQ
                     ANRAFPAGQVRNGGEAVSVTAGRTLSAATEIGLLALPSARGETVYVRDVATVLEAPRE
                     DQARAWRFARVGQDWSQAPAVSLAIAKRKGANAVVVSHAVLARVEALKGTLLPDSLSV
                     AVTRDYGETANEKANELLFHLGLATLSIVVLIGLAIGWREAAVTAVVIPTTILLTLFA
                     SNLMGYTINRVSLFALIFSIGILVDDAIVMIENIARHWAMNDGRSRIDAAVEAVAEVG
                     NPTVVATLTVVSALLPMLFVSGLMGPYMAPIPVNASAAMVFSFFVAVVIAPWLMVRFA
                     RKTLAAGGGHHGEGWLGAAYRRVAGGVIATRRSAWTFLIGVGVATLVACAMFATKTVT
                     VKLLPFDNKSELQVVVDLPEGTSLETTQRVLNDAAAITRTLEEVTAIDAYAGTASPFN
                     FNGLVRHYYQRNRPEMGDLSVALAPKDERKRSSHRVALDLRERLARLALPAGASIKVV
                     EAPPGPPVMATLLAEIYGPDATTRRAVAERVKAAFHAVPYIVDIDDSYGQPRPGLRLV
                     PDRSRLEALRVSDRDVFDSIGAALGGQVVGYAHRGEGRDPLEISVRLPQSARSWGEGL
                     AAMPVALSGDGRLVGLGEVVTASQEAGSTHVFRRDGRDVDMILGELAGAYEAPIYGML
                     AVDKAIKDGDWKNVPRPEIRLHGQPLDESRPTVLWDGEWEITWVTFRDMGAAFGVAIL
                     GIYVLVVAQFKSFRLPLVILTPIPLTLVGIVIGHILFRAPFTATSMIGFIALAGIIVR
                     NSILLVDFIRHSQGDGRPLREVLLEAGAIRFKPIVLTALAAMIGAAVILFDPIFQGLA
                     ISLLFGLASSTLLTVLVIPAIYVVLRDDTPASAPAGTS"
     gene            complement(6709..>7265)
                     /locus_tag="PLO65_06165"
     CDS             complement(6709..>7265)
                     /locus_tag="PLO65_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD27866.1"
                     /translation="RAASAELGAQGAILAPTSGRVLVADVPLGSVVMAGQSVARITAG
                     PPVVRIELPEGQARALKVGDEIRLSADDLGGAVTSGLVTQIYPSVTAGQVVADVTAAN
                     LPANLIGQRVRALVPIGQRQALVVPRRYVTTRYGIDYVRLVRSDASVSEVPVQTTAGP
                     SADAVEILSGIRAGDVLTPAGAAR"
BASE COUNT         1043 a         2452 c         2599 g         1161 t
ORIGIN      
        1 ggcctgcgcc aggccgagca gcgtcagcgc cagtcgcggc gcctcgccgg cccggcctcg
       61 gatcgccagg aaattgcggt cagccgcgag atcggaccgg ctcatccggc gacaaatgca
      121 taaatgcgca ttttggaata tattggaatt gcgcgcgggc ggcggcgggt gttgagtgcg
      181 gccgaacgag ggaactgagt catggaactg ggtgtcgtcg agctgtctcg gctgcagttc
      241 gccctgacgg cgatgtacca ctttctgttc gtgccgctca cgctcggact gtcgttcctg
      301 ctggtcatca tggagagcat ctatgtgatg accgggcggc cgatctggcg aacgatcacc
      361 cgattctggg ggacgctgtt cggtatcaat ttcgtgctgg gcgtcgccac cggcctgacc
      421 atggagttcg agttcgggac caactggtcc tactactccc actatgtcgg cgatgtcttc
      481 ggcgcgccgc tggccatcga ggggctgatg gccttcttcc tcgaggcgac cttcgtcggc
      541 ctgatgttct tcggctggga gcggctgtcg cgccgggggc acctgctggt gaccttcatg
      601 gtcgcgctgg ggtcgaacct gtcggccctg tggatcctga tcgccaacgg ctggatgcag
      661 aatccggtcg gggcggtgtt caatcccgac accatgcgga tggaggtcag cagcttccgc
      721 gacgtgctgt tcaatcccgt ggcccaggcc aagttcgtgc acaccgtcag cgcgggctac
      781 gtcacggcgg cggccttcgt gctcggtgtg tccgccctga tgctcctgcg gggacgctgg
      841 ccgcgggtgg cgaagcggtc gttcgccgtg gccgcggcgt tcggcctggc ctcggccctg
      901 tcggtcgtgg tgctcggcga cgagagcggc tacgccctga ccgacaacca gaagatgaag
      961 ctcgccgccc ttgaggcgat gtgggagacc gagccggcgc ccgccgggct gacggcgttc
     1021 ggggtgcccg acatggccag ccgcacgacg aaggccgaga tcaagattcc ctacgttctg
     1081 ggcctgatcg ccacccgcag cctcgacaag ccggtgatcg gcatcctgcc gctggtggcg
     1141 atcgccgaga cgcgcatcga gaacggcgtg aaggcctatg acgccctcga gcggctcaag
     1201 gccgcgccca ccgatccggc ggcccgggcc gatttcgaac tggcccgtgg ggacctcggc
     1261 tacggcctgc tgctcaagcg ctacgtggcc gacccccgca tggcggaccg cgcgacgatc
     1321 cagcgcgccg cctgggacac cgtgcccaac gtgccggtga tgttctgggc cttccgcgcg
     1381 atggccggca tcggcttcct gatgatcgcc gtgttcggcg ccgccttcgt gatggtctcg
     1441 ctgcggcgca gccaggcccg gtggctgctg tggctggcgg tcgtggtcat gcccctgccc
     1501 tggatcgcgg cggaggccgg ctgggttctg gccgagatgg gccgccagcc ctgggcggtg
     1561 gaaggcgtgc tgccgacctt cctggggacc tccagcctga cggcaggcca gatctggacg
     1621 acgatcatcg gcttcaccgt gatctacagc gccctggcgg tggtcgaggt cgggctgatg
     1681 gtccggacga tccgccgcgg cccctacgcg catcatgaag accccgacca ggccgaggcg
     1741 gtcgcgcccg tcgccgaccc cgccgcagag ggagcccgct gatggacatt ccgatcgact
     1801 acccgaccct gcggctcatc tggtggggcc tgatgggagt cctgctgatc ggcttcgccc
     1861 tgaccgacgg ctatgacctg ggcgtcaccg ccctgctgcc cttcgtggcc cgcgacgacg
     1921 gcgagcggcg gatggtgatc aatacggtca gcgcccactg ggaaggccac caggtgtggt
     1981 tcatcctcgg cggcggcgcg atcttcgccg cctggccgtt tgtctatgcg gtcagcttct
     2041 cgggcttcta tctggccatg ttcgccgtgc tcgcggcgct gatcctgcgg ccggtcgggt
     2101 tcaaataccg ctccaagcga ccgtcgcccc tctggcgcgg cagctgggac tgggcgctgt
     2161 tcgtcggcgg cttcgcgccg gccctggtgt tcggcgtcgc cgtgggcaat gtgctgcagg
     2221 gcctgccctt ccgcctcgac ggggacctgc gcgccttcta cgagggcacg ttccttggcc
     2281 tgttcacgcc gttcaccctg gtctgcggcc tgctgtcggt ggccatgctg gtgacgcacg
     2341 gcgcggcctg gctggcgatc agggccgagc caggcccggt gcgcgaccgg gcgcgccggt
     2401 tcggggccat cgcggcggcg gccgtgatcg tgctgttcgc ggccggttac gccttcgtcg
     2461 ccttcggtgg cttcggtttc cgcctagatg ggccggtcga tccggccgga ccatcgaacc
     2521 cgctgcgggc cggcgcggtg gccgccgccg gcggctggct gaccaacttc cggatctggc
     2581 cgtggatgtg gctggnnnnn nnnnncgatc ctcggcttcg gcggcgcgat cctggccctg
     2641 atcggcatcc gcggcgggcg cgaggcgctc gcgttcggcg ggtcatccct ggcggtgctg
     2701 ggggggatct cgacggtcgg cctgtcgatg ttcccgttca tcctgccgag cagcctcgac
     2761 cccgggtcca gcctgacggc ctggaacgcc tcgtccagcc acatgacgct gttcgtcatg
     2821 ctgatcgtga cggtggtctt cctgccgatg gtgctggcct acaccgcctg ggcgctcagg
     2881 gtgatgtggg ggcgttcgac catcgacgcc ctgtcgacca atcccgacct ctactgagct
     2941 ggagagcgat catgtggtac ttcacctggg tgctcggcct cggcctcgcg gtcgccttcg
     3001 gcatcctcaa cggcatgtgg cacgaattcg acctgatgga atccggcgat ctcggcgagg
     3061 acgacgtctg accggccgac tggctcaggt ctgcgcgcga tagatctcgc acagggtgtc
     3121 gagcacccgc atcgcggccg gatccgccag gcgatagtag atcgtctggg cgtcgcgccg
     3181 ggtggcgacc agcccctcgg cgcgcatctt ggccagatgc tgcgaggtcg ctgactgcgc
     3241 caggcccacg tgcgtcgcca ggtccccgac cgacgcctcg ccatcgacca gacggcacaa
     3301 cagcagcaac cgctgctcgc tggccatcat cttcagcaga cgggcggccg aatgggcgct
     3361 gtcggcgaga tccagcgcct cgcgggaggt ggggccggcg gccagaacgg tactcatctg
     3421 tctaatgtag aagatgcgca tggacgatca agggcgtccg gcggcgacag gcccggggtc
     3481 cgccaagggg cggaccccgt cccggtcagg acgtcccggc gggcgccgag gccggcgtgt
     3541 cgtcgcgcag gacgacgtag atcgccggga tgaccagcac ggtcagcagc gtcgaggagg
     3601 ccaggccgaa cagcagcgag atcgccagac cctggaagat cgggtcgaac aggatcaccg
     3661 cggccccgat catcgcggcc agggcggtca gcacgatcgg cttgaagcgg attgccccgg
     3721 cctcaagcag aacctcgcgc aacggccggc catcgccctg gctgtgccgg atgaagtcca
     3781 ccagcaggat cgagtttcgc acgatgatcc cggccagggc gatgaagccg atcatcgagg
     3841 tggcggtgaa cggtgcccgg aacaggatgt ggccgatgac gatgccgacc agggtcagcg
     3901 ggatcggcgt gaggatcacc agcggcaggc ggaagctctt gaactgggcg acgaccagca
     3961 catagatgcc caggatcgcc acgccgaagg ccgcgcccat gtcgcggaag gtcacccagg
     4021 tgatctccca ttcgccgtcc cacagcacgg tcggccgact ctcgtcgagc ggctggccat
     4081 ggaggcggat ttcgggccgc gggacgttct tccagtcgcc gtccttgatc gccttgtcga
     4141 cggccagcat gccgtagatc ggcgcttcat aggcgccggc cagctcgccg aggatcatgt
     4201 ccacgtcacg accgtcgcgg cggaagacgt gcgtcgagcc ggcttcctgg ctggcggtga
     4261 cgacctcgcc caggccgacc agccgcccgt cgccggacag ggccacgggc atggccgcca
     4321 ggccttcgcc ccaactgcgc gccgactgcg gcaaccgaac ggaaatctcc agcggatcgc
     4381 gcccctcgcc gcggtgggcg tagccgacca cctgaccgcc cagggcggcg ccgatggaat
     4441 cgaacacgtc gcgatcgctg acccgcagcg cctccaggcg gctgcggtcg gggaccaggc
     4501 gcaggccggg ccggggctgg ccatagctgt cgtcgatgtc gacgatgtag ggcaccgcat
     4561 ggaaggcggc cttgacccgc tcggccacgg cccggcgcgt cgtggcgtca gggccgtaga
     4621 tttcggccag cagggtcgcc atcaccggcg gcccgggcgg cgcctcgaca accttgatcg
     4681 acgcgccggc cggcagggcc agcctggcca gcctttcgcg caggtcgagg gccacgcggt
     4741 ggctcgaccg cttgcgctcg tccttgggcg ccagggcgac ggacaggtcg cccatctcgg
     4801 gcctgttgcg ctggtagtag tgccggacca ggccgttgaa gttgaacggc gaggcggtcc
     4861 cggcgtaggc gtcgatcgct gtgacctcct ccagcgtccg ggtgatggcg gccgcgtcgt
     4921 tgagcacccg ctgggtggtc tccagcgagg tcccctcggg aagatcgacg accacctgca
     4981 gctcggactt gttgtcgaac ggcagcagct tcacggtcac ggtcttggtc gcgaacatgg
     5041 cgcaggcgac cagcgtggcc acgcccaccc cgatcaggaa ggtccaggcg ctgcgacgtg
     5101 tggcgatcac gccgccggcc accctgcgat aggcggcgcc cagccagccc tcgccatgat
     5161 ggccgccgcc ggcggccagg gtcttgcgcg cgaaccgcac catcagccac ggggcgatga
     5221 ccacggcgac gaagaaggag aataccatcg ccgccgaggc gttgaccggg atcggggcca
     5281 tgtaggggcc catcaggccc gacacgaaca gcatcggcaa cagggccgag acgacggtca
     5341 gggtggccac cacggtcggg ttgccgacct cggcgaccgc ctcgacggcc gcgtcgatcc
     5401 ggcttcgtcc gtcgttcatg gcccagtgcc gggcgatgtt ctcgatcatc acgatggcgt
     5461 cgtcgaccag gatgccgatc gagaagatca gggcgaacag gctgacgcgg ttgatcgtgt
     5521 agcccatcag gttggaggcg aagagcgtca gcaggatggt cgtgggaatg accaccgccg
     5581 tcaccgccgc ttcgcgccag ccgatggcca ggccgatcag cacgacgatc gacaacgtcg
     5641 ccaggccgag gtggaacagc agttcgttgg ccttctcgtt ggccgtctcg ccatagtcac
     5701 gggtcaccgc gacgctcagg ctgtcgggca gcaacgtgcc cttgagcgct tcgacacggg
     5761 ccagcacggc gtgggacacg acaacggcgt tggccccctt gcgcttggcg atggccaggc
     5821 tgaccgccgg ggcctggctc cagtcctggc ccacccgggc gaaccgccag gcgcgggcct
     5881 gatcctcgcg cggcgcctcc aggacggtgg ccacgtcgcg cacatagacg gtctcgcccc
     5941 tggccgaggg cagggcgaga aggccgattt cggtggctgc ggacagggtc cggccggcgg
     6001 tcacgctgac cgcttcgccg ccgttgcgca cctggccggc cgggaaggcc cggttggcct
     6061 gacggatggt ctccatcaga ccgcccagcg agacgccgtg catcgccagg cgcgcggggt
     6121 ccggctcgac ccgtatctcc tgcggtcggc cgccgacgat gaaggtcagc ccgacgtcat
     6181 cgaccttggc gacctcggtg cgcagcttgc cggccagttc gtagagcgcc tggtcgctcc
     6241 attgtccggc ggcgccgggc ttgggcgaca gggtcaggac caggcttgga acgtcgttga
     6301 tgccgcgggt ctggaccagc ggctcggcga tgccggcggg tatccggtcg aggttggcct
     6361 ggatgcgctc atggatgcgc acggccgcgg cgtcggggtc gacgccgacc ttgaaccggg
     6421 cggtgaccat cacctggtcg tcgtcggcga aggtgtagac gtgttcgacg tcgtggatgc
     6481 tcttgacgat ggtctcgagc ggcttgccga ccagttcgac ggcgtctggc gcccgcaggc
     6541 cgggcgcggc gaccatgatg tcgaccatcg gcacgctgat ctgcggctcc tcctcccggg
     6601 ggatgctgac cagggccagc aggccgacgg cgatggcggc gagcaggaac aggggcgtca
     6661 ggggggaccg gatggtggcc cgcgtcagcc gaccggaaag gccgaggttc atcgcgccgc
     6721 tcccgccgga gtcagcacgt cgccggcccg gatgccggac aggatttcca ccgcgtcggc
     6781 cgacggcccg gcggtggtct gcaccgggac ctcggagacg gacgcgtcac tgcgcaccag
     6841 ccgtacatag tcgatgccat agcgggtggt cacatagcgg cgcggtacga caagggcctg
     6901 ccgctggccg atcggcacca gggcgcggac gcgctggccg atcaggttgg ccggcaggtt
     6961 ggcggcggtc acgtcggcga ccacctggcc ggcggtcacc gacggataga tctgcgtcac
     7021 caggccggag gtgacggcgc cgccgagatc atcggcggac agccggatct cgtcgccgac
     7081 cttcagcgcg cgggcctggc cctcgggcag ttcgatacgc acgaccggcg gacccgcggt
     7141 gatccgggcg accgactggc cggccatcac gaccgagccg agcggcacgt cggcgaccag
     7201 cacgcggccg gaggtcggcg ccaggatggc gccctgggcg ccgagctcgg cgctggcggc
     7261 gcgtt
//
LOCUS       DAORNX010000103         7227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7666_length_7227_cov_7.684746, whole genome shotgun sequence.
ACCESSION   DAORNX010000103 DAORNX010000000
VERSION     DAORNX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7666_length_7227_cov_7.684746"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..1807
                     /locus_tag="PLO65_06170"
     CDS             392..1807
                     /locus_tag="PLO65_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRD27867.1"
                     /translation="MYSAAGEVRDHYGSVHARLTTLSPEALADRQKTLERSFLLQGIT
                     FTVYGAETTTERIIPTDLVPRIIPSREWDHIEAGLIQRLKALNMFLDDIYGEQRILMD
                     GIVPRELVLGAPSYRREMQHLYVPHKAYANVCGSDLIRDQDGAFAVLEDNLRVPSGVS
                     YMLANRDAAKRTFPGTFRAANVRPVERYPDLLLATLKSMAPDWRPDPQVVVLTPGVYN
                     SAYYEHAYLARLMGAPLVEGRDLVVHENMVYMRTTTGLRRVDVIYRRVDDDFIDPLTF
                     RRDSGLGVAGLFNAYRAGNVVMCNAPGTGVADDKAVYAYVPDIIRYYLGEDAILPNIE
                     TFLCREPAQLNHVLANLDRLVVKAVGASGGYGMLVGPHASRKEREAFAEALKADPANY
                     IAQPTIQLSTAPCLIDGRIEPRHVDLRPFILSGEKIVVTPGALTRVALRRGSLVVNSS
                     QGGGSKDTWVLVDETSEGVRP"
     gene            1804..2748
                     /locus_tag="PLO65_06175"
     CDS             1804..2748
                     /locus_tag="PLO65_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRD27868.1"
                     /translation="MMLARVADSLYWIGRYLERAEHFSRLADVMLNATLDRSDAAAQT
                     AYIALSAVGDPDMARAARTYEAAKSLVLDRDDPNSVVISLSLARENARQVRDQITTET
                     WERLNLLYLRVTSDGAADAFESGSSAYIHDIIADLHLFKGAADATMSHGEGWRFLMLG
                     AYLERAQLIARLLEVCFGDGRDGHVTDRIALMSLLRMGCALEPYLRRYTAEIETRHIL
                     EFLVFDEEFPRSIRFATTRIEEHLVGVSRHVESGGLATPERLAGRLKARLQYADVDEL
                     ESVGASALLATVIGECARIHDAVFDTFVAYSLEMKLPA"
     gene            2748..3725
                     /locus_tag="PLO65_06180"
     CDS             2748..3725
                     /locus_tag="PLO65_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRD27869.1"
                     /translation="MLLEVRHVTQYHYERPVRESVMELRMQPQKSARQRLISFELDLD
                     PRAQLFSYVDSFGNAVFHFDVPQAHDTLRIESRSAVETQPDPILPDALDMGEWDRLRS
                     DFVRGECFDFLRPHGFATPSAALDTFVQEQNLDRLRHRDPLTALRALTEALYTAFDYA
                     PGVTTADSPIDLALGVGRGVCQDFAHIMIAICRSWGIPTRYVSGYLFTDRQHGDRSDP
                     DATHAWVEAFLPTLRWTGFDPTNNMLTGERHVAVATGRDYADVPPSLGVYKGEADSQL
                     SVAVAVARARAAIAEPEFLRMARPTFPGGRRRASDEARRRQEQQQQQQQ"
     gene            complement(3787..4308)
                     /locus_tag="PLO65_06185"
     CDS             complement(3787..4308)
                     /locus_tag="PLO65_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27870.1"
                     /translation="MAMDIPNFGVPDDTLVAVVLGALLATLGGFLAARFEAWLRRRER
                     ERDAALLLGEIVFTFVTVLKMTDETRRIGDPYGPITMQMLAGARREADAYERHRDRLP
                     DLTDPDLRLGMHRLMITLTMTLDGMLATAPVAGAPRPGLGGRRPRRCRPSATAPLTSS
                     WSRFLRSIRCSSV"
     gene            complement(4380..4454)
                     /locus_tag="PLO65_06190"
     tRNA            complement(4380..4454)
                     /locus_tag="PLO65_06190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4420..4422),aa:Lys,seq:ttt)
     gene            4570..5199
                     /locus_tag="PLO65_06195"
     CDS             4570..5199
                     /locus_tag="PLO65_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27871.1"
                     /translation="MRKLVVVLLSPLTGLGGCATAAAQPQPQPSSPPPAQVQTTDQIN
                     RGSVVGAVSAPMRDLNLLRSKIPPVLLEALEDPYRRPRPATCGELVAQLRPLNDALGA
                     DIDEPSTPDDRALIAKGRDVAGETALGMVASAAQDLIPLRGWVRKLSGAEQHDRLVRA
                     AITAGGIRRGYLKGLGEARGCNPPATPTHVKSEIPPPPPKKKGPRFPIR"
     gene            complement(5232..6341)
                     /gene="tgt"
                     /locus_tag="PLO65_06200"
     CDS             complement(5232..6341)
                     /gene="tgt"
                     /locus_tag="PLO65_06200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRD27872.1"
                     /translation="MATFPFDISATDGAARTGVLKTPRGDIRTPAFMPVGTAGTVKAL
                     TVDQVARTGADIILGNTYHLMLRPTAERVKRLGGLHRFMRWDKPILTDSGGFQVMSLS
                     GISKVTEEAVTFSSHLDGSKHVLTPERSMEIQADLLGSDIVMQLDECVAWPAEEARAR
                     QGMELSARWGARSKAAFGERDSQALFGIQQGSTFETLRRESSERLVETGFDGYALGGL
                     AVGEGHEAMCEVLDYAPAMLPADRPRYLMGVGKPIDLVEAVYRGVDMFDCVLPTRSGR
                     HGQAWTWSGPLNMKNARFAEDDTPLDETSACPASSGYSKAYLHHLVRSEEILGQVLLS
                     WHNIAFFQALTAAMRAAIAEGRFEAFRRDFRAGLQ"
     gene            complement(6341..>7227)
                     /gene="queA"
                     /locus_tag="PLO65_06205"
     CDS             complement(6341..>7227)
                     /gene="queA"
                     /locus_tag="PLO65_06205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRD27873.1"
                     /translation="REARADGGGDGHAVAVEATLHRRLSQNRWSAFMRPGKRLKVGDR
                     VRFGAATDRACEGASLDATLVAKGEGGEVELAFDLSGPDLDIAINSRGHMPLPPYIAA
                     RRPEDEQDRSDYQTIYAREDGSVAAPTAGLHFTPELLTRLAAAGVSQHFVTLHVGAGT
                     FLPVKVDDLADHRMHAEYGEVSAETAAALNAVRAAGGRIVCVGTTSLRLLESATDEDG
                     VINAWSGETAIFITPGYRFRAADGLMTNFHLPRSTLFMLVSAFSGYDTMKAAYAHAIA
                     TDYRFYSYGDSSLLWRAA"
BASE COUNT         1122 a         2582 c         2449 g         1074 t
ORIGIN      
        1 ggggcgtctg cgacaacgcg ccgcccgccg gcgcgaagag cgcggcgacg gccgaggcgg
       61 caagggcgaa gcgcgtcggc gccaaactgt cttcctctgt ctgcgccccg gcccccgttt
      121 agtcgggcgc ggcgcggctg tcacgccatc cagccctttg cacgggcggt gaaagttgca
      181 caaatgccgg gcagtcggag cttccggcgg cgactcgcgc cgaaacgggt ccggcagctt
      241 gcgaagagcg ttaggaatga accgtgaaga ggccgttcag gccgccaagg aaacggaccc
      301 tggcttgacc atcgcgagcg tgaggacagc cgacattggc cagggcgcat cgaccccggc
      361 cgcctacctg cccggccagg cgttcgacga gatgtattca gcggccggcg aggtgcgcga
      421 ccactacggc tcggtccacg cccgcctgac caccctgtcg ccggaggccc tggccgaccg
      481 gcagaagacg ctcgagcgct cgttcctgct gcagggcatc accttcaccg tctacggcgc
      541 cgagacaacc accgagcgga tcattcccac cgatctcgtc ccgcggatca ttccgtcgcg
      601 cgagtgggac catatcgagg ccgggctgat ccagcgcctg aaggcgctga acatgttcct
      661 cgacgacatc tacggcgagc agcggatcct gatggacggg atcgtgccac gcgagctggt
      721 gctgggggcc ccgtcctatc gccgcgagat gcagcacctg tatgtgccgc acaaggccta
      781 cgccaacgtc tgcgggtcgg acctgatccg cgaccaggac ggcgccttcg ccgtgctgga
      841 ggacaatctg cgggtgccgt cgggcgtctc ctacatgctg gccaaccgtg acgcggccaa
      901 gcggaccttc cccggcacct tccgcgcggc caacgtgcgg ccggtcgagc gctaccctga
      961 cctgttgctg gcgacgctca agagcatggc gcccgactgg cggcccgacc cgcaggtcgt
     1021 ggtgctgacg ccgggcgtct acaacagcgc ctattacgag cacgcctatc tcgcccggct
     1081 gatgggcgcg ccgctggtcg agggccgcga cctcgtggtg cacgagaaca tggtctacat
     1141 gcgcaccacc accggcctgc gccgggtgga cgtgatctac cgccgtgtcg acgacgactt
     1201 catcgacccc ctgaccttcc ggcgcgactc gggcctcggc gtagccgggc tgttcaacgc
     1261 ctaccgcgcc ggcaatgtgg tgatgtgcaa cgccccggga accggcgtgg ccgacgacaa
     1321 ggcggtctat gcctatgtcc cggacatcat tcgctactac ctgggcgagg acgccatcct
     1381 gcccaatatc gagaccttcc tctgccgcga gccggcgcag ctgaaccatg tcctggccaa
     1441 tctcgacagg ctggtggtca aggctgtcgg cgcctcgggc ggctacggca tgctggtggg
     1501 tccgcacgcc agccgcaagg agcgcgaggc cttcgccgag gccctgaagg ccgatccggc
     1561 caactacatc gcccagccga ccatccagct gtcgaccgcc ccctgcctga tcgacggccg
     1621 catcgagccc aggcacgtgg acctgcggcc gttcatcctg tcgggcgaaa agatcgtggt
     1681 gacgcccggc gccctgacgc gggtggcgtt gcggcgcggc tcgctggtgg tcaattccag
     1741 ccagggcggc gggtcgaagg atacctgggt gctggtcgac gagacctcgg agggcgtccg
     1801 gccatgatgc tcgcgcgggt cgccgacagt ctttactgga tcggccgcta tctggagcgg
     1861 gccgagcatt tctcgcgcct ggccgacgtg atgctcaacg ccacgctcga ccggtcggac
     1921 gcggcggccc agaccgccta catcgccctg tcggcggtcg gtgaccccga catggcccgg
     1981 gcggcgcgga cctacgaagc ggccaagtcg ctggtgctcg accgggacga tcccaattcg
     2041 gtggtcatct ccctgtcgct ggcccgggag aacgcccggc aggtgcgcga ccagatcacc
     2101 accgagacct gggagcggct caacctcctc tacctgcgcg tgaccagcga cggggccgcg
     2161 gacgccttcg agagcggctc gtccgcctat atccacgaca tcatcgccga cctgcacctg
     2221 ttcaagggcg ccgcggacgc caccatgagc cacggcgagg gctggcggtt cctgatgctg
     2281 ggggcctacc tcgagcgggc gcagctgatc gcccggctgc tggaggtctg tttcggcgac
     2341 gggcgcgacg gccacgtgac cgatcgtatc gccctgatga gcctgctgcg catgggctgc
     2401 gccctggagc cctatctgcg gcgctacacc gccgagatcg agacccggca cattctcgag
     2461 ttcctggtct tcgacgagga attcccccgc tcgatccgct tcgccaccac gcgcatcgag
     2521 gaacatctgg tcggggtgtc ccgccatgtc gaatccggtg ggctggccac gcccgaacgc
     2581 ctcgccggac ggctcaaggc gcggctgcag tacgccgatg tcgacgagct ggaatcggtt
     2641 ggagccagcg ccctgctggc cacggtgatc ggcgaatgcg cccgcatcca cgacgccgtc
     2701 ttcgacacct tcgtggccta ttccctcgag atgaagcttc cagcctgatg ttgttagaag
     2761 tccgccatgt cacccagtac cactacgagc gcccggtccg ggaaagcgtc atggaactgc
     2821 gcatgcagcc ccagaagagc gcccgccagc ggctgatcag cttcgagctg gacctcgatc
     2881 cgcgcgccca gctgttctcc tacgtcgaca gtttcggcaa cgcggtgttc cacttcgacg
     2941 tgccccaggc gcacgacacc ctgcgcatcg aatcgcgctc tgcggtcgag acccagcccg
     3001 acccgatcct gcccgacgcc ctcgacatgg gcgaatggga ccggctgcgc agcgatttcg
     3061 tgcgcggcga atgcttcgac ttcttgaggc cgcacggctt cgcgaccccg tcggcggcgc
     3121 tcgacacctt cgtgcaggaa cagaacctcg acaggctgcg ccatcgcgac ccgctgaccg
     3181 ccctgcgggc gctgacagaa gcgctctaca cagccttcga ctacgcgccg ggcgtgacca
     3241 cggccgacag tccgatcgac ctggcgctcg gcgtcggccg cggggtctgt caggacttcg
     3301 cccacatcat gatcgccatc tgccgcagct ggggcatccc gacccgctat gtctccggct
     3361 acctgttcac cgaccgccag cacggcgacc ggtcggaccc cgacgcgacc cacgcctggg
     3421 tcgaggcctt cctgccgacc ctgcgctgga ccggattcga cccgaccaac aacatgctca
     3481 ccggcgagcg ccatgtcgcc gtggccaccg gccgcgacta tgccgacgtt ccgccgtccc
     3541 tgggtgtcta caagggcgag gccgacagcc agctgtcggt ggccgtggcc gtggcccggg
     3601 cccgggcggc gatcgccgag cccgaattcc tgcgcatggc ccggccgacc tttcccggcg
     3661 gacggcggcg cgcgtcggac gaggcccgcc gacggcagga acagcagcag caacagcagc
     3721 aatagccggc cgatcaggcc cggacggccg cttcatggcc gtcgaaggac acgcgcgcca
     3781 gccggctcag acgcttgagc agcggatcga gcgcaggaac cgactccatg atgaagtcaa
     3841 aggcgctgtc gcgctgggcc tgcagcgcct gggccgccgc ccgcccagcc ctggtcgcgg
     3901 agcgccggcg accggagccg tggccagcat gccgtcgagc gtcatggtca gggtgatcat
     3961 cagcctgtgc atgcccagcc tcagatccgg gtcggtcagg tccggcagcc ggtcccgatg
     4021 gcgctcgtag gcgtccgcct cgcgccgcgc gccggcgagc atctgcatgg tgatcgggcc
     4081 gtagggatcg ccgatccggc gggtctcgtc ggtcatcttc aacacggtca cgaaggtgaa
     4141 gacgatctcg cccagcagca gggcggcgtc ccgctcgcgc tcgcgccgcc gcagccaggc
     4201 ctcgaaccgc gcggcgagga acccgcccag ggtcgccagc agcgcgccga gcacgaccgc
     4261 gaccagggta tcgtcgggta cgccgaagtt ggggatgtcc atggccatgg ccgacagtcc
     4321 gacaggatcg gcgacttgtc acggggcgcc ccgccccgcg ggccgcctgc cgtcacaatt
     4381 ggtaggccgg gtggggatcg aacccacgac cattcgatta aaagtcgaat gctctaccgc
     4441 tgagctaccg gcccgctcac gcggcgccgc gaggcgctcc cgaaagcggc gcggaccata
     4501 gtggcgggcc catcccgccg caagcgccga acgctttgcc tggcgcggcc tccgggttag
     4561 catccgggca tgcgcaagct cgtcgtcgtt ctcctctccc cgttgaccgg cctcggcggc
     4621 tgcgccaccg cggcggcgca gccgcaaccc cagccttcgt ccccgccccc cgcgcaggtg
     4681 cagaccaccg accagatcaa ccgcggcagc gtcgtcgggg ccgtcagcgc gccaatgcgc
     4741 gacctgaacc tgctgagatc gaagattccg ccggtgctgc tggaagcgct ggaggatccc
     4801 tacaggcggc cccggccagc cacctgcggc gagctggtcg cccagctccg accgctcaat
     4861 gatgcgctcg gagccgacat cgacgagccg tcgacgcccg atgaccgggc cctgatcgcc
     4921 aaggggcggg acgtcgccgg cgaaacggcg ctgggcatgg tcgcctcggc ggcccaggac
     4981 ctgatcccgc tgcggggctg ggtgcgcaag ctcagcggcg ccgaacagca cgaccggctg
     5041 gtgcgcgcgg ccatcacggc cggcggcatc cgtcgcggct atctgaaggg ccttggcgag
     5101 gcgagaggct gcaatccgcc ggccacgccg acccatgtaa agtcggaaat ccccccgccg
     5161 ccgccgaaga agaagggccc gcgcttcccg atccggtagc ggaggggaca cgtacctctg
     5221 gtacatgtcc cctattgcag gccggccctg aaatcccggc ggaaggcctc gaaacggcct
     5281 tcggcgatcg ccgcgcgcat agccgccgtc agggcctgga agaaggcgat gttgtgccag
     5341 ctcagcagca cctggccgag aatctcctcc gagcggacca ggtggtgcag gtaggccttc
     5401 gaatagccgc tgctggccgg gcaggcgctt gtctcgtcga gcggcgtgtc gtcctccgcg
     5461 aaacgggcgt tcttcatgtt cagcggcccc gaccaggtcc aggcctggcc gtggcggccc
     5521 gagcgggtcg gcaggacgca gtcgaacatg tcgacgccgc gatacaccgc ctcgaccagg
     5581 tcgatcggct tgcccacgcc catcaaatag cgcggccggt cggccggcag catggcaggg
     5641 gcgtaatcga gcacctcgca catcgcctca tgcccctccc cgaccgccag gccgccgagg
     5701 gcgtagccat caaagccggt ctcgaccagg cgctcggagg attcccggcg cagggtctcg
     5761 aacgtcgagc cctgctggat gccgaacagg gcctgggaat cgcgctcccc gaaggccgcc
     5821 ttcgagcgcg cgccccagcg ggccgacagc tccatgccct ggcgggcgcg ggcctcctcc
     5881 gccggccagg cgacgcattc gtccaactgc atgacgatgt cgctgcccag caggtcggcc
     5941 tggatctcca tcgaccgctc gggcgtcagg acatgcttgc tgccgtcgag gtggctggag
     6001 aaggtgaccg cctcctcggt gaccttgctg atgccgctca ggctcatcac ctggaagccg
     6061 ccgctgtcgg tcaggatcgg tttgtcccag cgcatgaagc ggtgcagccc gcccagccgc
     6121 ttcacccgct cggccgtcgg ccgcagcatc aggtgatagg tgttgccaag gatgatgtcg
     6181 gccccggtcc gggcgacctg gtcgacggtc agggccttga ccgtgcccgc cgtgccgacc
     6241 ggcatgaagg ccggtgtgcg gatgtcgccg cgcggcgtct tcaggacgcc ggtgcgggca
     6301 gcgccgtcgg tggcggagat gtcaaagggg aaggtggcca tcaggcggcg cgccatagca
     6361 ggcttgaatc gccgtaggaa tagaagcggt agtccgtggc gatggcgtgg gcgtaggcgg
     6421 ccttcatggt gtcgtagccg ctgaaggcgc tgacgagcat gaacagggtc gatcggggca
     6481 ggtggaaatt ggtcatcagg ccgtcggcgg cgcggaagcg gtagccgggt gtgatgaaga
     6541 tcgccgtctc gccggaccag gcgttgatca cgccgtcctc gtccgtcgcg ctttccagca
     6601 ggcgcagcga cgtggtgccg acgcagacga tccggccgcc ggccgcccgc acggcattga
     6661 gcgcggccgc cgtttccgcc gacacctcgc cgtattccgc atgcatccgg tggtcggcca
     6721 ggtcgtcgac cttgaccggc aggaacgtcc cggcccccac atgcagggtg acgaaatgct
     6781 gcgacacccc ggcggccgcc agccgcgtga gcagctccgg cgtgaaatgc aggcccgccg
     6841 tcggcgcggc gaccgagccg tcctcgcggg cgtagatcgt ctggtagtcg gagcggtcct
     6901 gctcgtcctc ggggcgcctg gccgcgatgt agggcggcag cggcatgtgg ccccggctgt
     6961 tgatggcgat gtcgagatcg ggtccggaca ggtcgaacgc cagctcgacc tcgccgccct
     7021 cgcccttggc gaccagggtc gcgtcgagcg aggccccttc gcaggcccga tcggtcgcgg
     7081 cgccgaagcg cacgcggtcg ccgaccttca gccgcttgcc gggccgcatg aaggcgctcc
     7141 agcggttctg ggacaggcgc ctgtgcagcg tggcctcgac cgccacggcg tgaccgtcgc
     7201 cgccgccgtc ggcgcgcgcc tcgcggc
//
LOCUS       DAORNX010000104         7216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7677_length_7216_cov_7.899595, whole genome shotgun sequence.
ACCESSION   DAORNX010000104 DAORNX010000000
VERSION     DAORNX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_7216_cov_7.899595"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLO65_06210"
     CDS             <1..408
                     /locus_tag="PLO65_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRD27874.1"
                     /translation="EARLASALAAARSRDAAAGRALTGPHRGDLAVIHAEKDRPAAEC
                     STGEQKALILNLILAQAARLFAAESAPAPVLLLDEVAAHLDPLRRAALADEIAALGLQ
                     AFLTGTDESLFDPFKGRALGVRVEASGLTVLDR"
     gene            409..2835
                     /gene="gyrB"
                     /locus_tag="PLO65_06215"
     CDS             409..2835
                     /gene="gyrB"
                     /locus_tag="PLO65_06215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRD27875.1"
                     /translation="MTDETEDHGAPQAHGEYDADSIKVLKGLDAVRKRPGMYIGDTDD
                     GSGLHHMVYEVVDNAIDEALAGHATRVEVILNADGSCTVTDDGRGIPVDMHEGEGVSA
                     AEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLELKIHRNGKAHQMRFERG
                     DAVSSLKITGDSPLRDNGKPLTGTSVTFYPSSTTFAFIEFDRKTLEHRLRELAFLNSG
                     VTIHFRDLRDAEPWEEVLHYEGGIEAFVKHLDKAKTALIGAPIAVRGVREKVEIDLAL
                     QWNDSYHEHMLCFTNNIPQKDGGTHLSAFRAALTRVITGYAESSGMARKEKVSVTGED
                     AREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGLVQEGLATWFEEHPAEARAIV
                     SKIAEAASAREAARKARELTRRKSALDITSLPGKLADCQERDPAKSELFIVEGDSAGG
                     SAKQARNRENQAVLPLRGKILNVERARFDKMLSSEQVGTLIMALGAGIGRDEFDIAKM
                     RYHRIVIMTDADVDGAHIRTLLLTFFYRQMPEVIERGYLYIAQPPLYKASKGKSSRYL
                     KDDAEYEAFLIDEGVEGAELDLSSGERMTGKDLLALVQTSRAAKANIERLSARAPSFA
                     IEQTALAGLMGERPDPAEAARRLNLYAQEGDGPWSGERGETGYVFSRVKRSVSEKIVL
                     EDALLQAADARRLAERAALLADTFQGQAVFRRKDRSTPVRGPLDLFAAVMDAGRKGLS
                     IQRYKGLGEMNPEQLWETTLDSEARTLLQVKVNHADDADDMFARLMGELVEPRRDFIQ
                     ANALDAEVDV"
     gene            complement(2832..3116)
                     /locus_tag="PLO65_06220"
     CDS             complement(2832..3116)
                     /locus_tag="PLO65_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27876.1"
                     /translation="MTDQPHDHETTPAARTATGLDATGLRASGAAATGATALGMAAIG
                     MGLLGALTVARMAIGRLTIGRLKVKTLMVKRLEVDELTVKRLKTEAPTKD"
     gene            complement(3194..>4045)
                     /locus_tag="PLO65_06225"
     CDS             complement(3194..>4045)
                     /locus_tag="PLO65_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRD27877.1"
                     /translation="GLPAEGITLYGSADMAWALRRIAGREVSEEVRQVQTRKVRQLYA
                     MLDGVSCRAAAVRRYFGEEGVEACGVCDLCLAPPGSVDATVAAQKALAAVHRLGGRFG
                     RGRLVDHLLGKTKDVHDSEAALSTYGVGKEFSPAGWRDLIDQLLFDGLLREDPNDGRP
                     LIGLGDGEGVKAVYRGERRIAVRRQPEGLDPSTRSGRPRKRTREAMETFAGDDLALFE
                     ALRAWRRDEAHEQHVPPYVIFGDRTLQDIVRLRPRSPDELLLATGVGDSKLKRYGAAV
                     LRVVKDN"
     assembly_gap    4046..4096
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4097..5025)
                     /locus_tag="PLO65_06230"
     CDS             complement(<4097..5025)
                     /locus_tag="PLO65_06230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRD27878.1"
                     /translation="MTDSLAPARDLLRRVFGHAEFRGLQAAVIAEILAGRSAMAVLPT
                     GGGKSLCYQIPAMLRPGLGLVVSPLIALMADQVEGLKQSGVAAERLDSSHSPDERAEV
                     WRRAERGELDLLYLSPEGLMLGATLERLRRIPLAIAAIDEAHCVSQWGHDFRPEYRML
                     GRLAEVFPDVPRLAVTATADARTRDDIRTELRLRDCAEFVDSFARPELALSAERKRGQ
                     GHGRVEELVLARPGRSGVVYTGSRDGTDRLAERLVAAGVPALAYHAGLERTQRTERLE
                     RFLEADAAVMVATIAFGMGVDKPDVRFVIHADPP"
     gene            5194..7143
                     /locus_tag="PLO65_06235"
     CDS             5194..7143
                     /locus_tag="PLO65_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRD27879.1"
                     /translation="MANQQGVKRTPLQAFLYWGAVVGVWGLIFLVAFFAVFATDLPDV
                     SKLNTVTRQPSISYLDRSGALIAVRGSQYAPPVDLDKLPDYVPKAFIAIEDRRFYYHP
                     GFDPIGISRAIVKDISCWCVAAGGSTITQQLARNLFLTLDQTPRRKAQELILAMWLEM
                     KFSKKQILALYLNRVYFGAGAYGIEAAAQRYFNKPASRLTLGEAALLAGMMKGPSRYS
                     PVSATERAARRATIVLDEMVRTRAITREQRAEAFRNPVRVSAVLANQRAQYFTDWVDA
                     QVRSLVGEPTEDLIVETTLDLPIQAAAESAVRSGVVGAKGQGVRQAALVAIDGEGRIR
                     AYVGGADYGESQFDRATSARRQAGSAFKPFVYLAAMEAGRTPAVMVVDEPVRIGSWEP
                     RNYTGRYLGAITLQTALAQSVNTVAARLANEVGTGNVAAAARRLGITSKIQLDPSMAL
                     GAVEVNPLEMAQSYAAFSNGGFLAKGYGIQRIRTANGRVLYDRNLEKRPRRSVIGSPA
                     LQYMNQMMREVVISGTGKRARVPNYDIAGKTGTTSDYRDAWFVGYTGGFVTAVWTGKD
                     DNTPMKGVTGGGAPAGIWRAFMAQALPRLKAQAIPGGYAPVEEPRDAIGDILSTLGMG
                     GDSGAPATPAPDARTSPRVPEEIPY"
BASE COUNT         1134 a         2559 c         2463 g         1009 t
ORIGIN      
        1 gaggcccggc tggcgtcagc cctggccgcc gcgcgcagcc gcgacgcggc cgccggcagg
       61 gccctgaccg gccctcaccg gggcgatctg gcggtgatcc acgcggaaaa ggaccggccc
      121 gccgccgaat gttcgaccgg cgagcagaaa gccttgattc tgaaccttat tctggctcag
      181 gccgcgcgac tttttgctgc ggaatcagcg ccggctcctg tattgttgct cgacgaagtc
      241 gccgcccatc tcgacccgct ccggcgggcc gcgctggcgg atgaaatcgc ggcgctcggc
      301 ctgcaggcat tcctgaccgg aacggacgag tcgctgttcg accccttcaa gggtcgggcc
      361 ctcggcgtcc gtgtggaagc ctcgggcctg accgttctgg accgctgaat gaccgacgaa
      421 accgaagacc acggcgcacc gcaggcgcac ggcgaatacg acgccgactc gatcaaggtg
      481 ctcaagggcc tggacgcggt gcgcaagcgc ccgggcatgt acatcggcga caccgacgac
      541 ggcagcggcc tgcaccacat ggtctacgag gtcgtcgaca acgccatcga cgaggccctg
      601 gccggtcacg cgaccagggt cgaggtgatc ctcaacgccg atggctcctg cacggtcacc
      661 gacgacggcc gcggcattcc cgtggacatg cacgagggcg agggcgtcag cgccgccgaa
      721 gtgatcatga cccagctgca cgcgggcggg aaattcgacc agaacagcta caaggtctcc
      781 ggcggcctgc acggcgtggg cgtctcggtg gtcaacgcgc tcagcgactg gctggagctg
      841 aagatccatc gcaacggcaa ggcgcaccag atgcgcttcg agcgcggcga cgcggtcagt
      901 tcgctgaaga tcaccggcga ctcgccgctg cgcgacaacg gcaagccgct gaccggcacc
      961 agcgtgacct tctatccgtc gtcgaccacc ttcgccttca tcgaattcga ccggaagaca
     1021 ctcgagcatc gcctgcgcga gctggccttc ctcaacagcg gggtgacgat ccacttccgc
     1081 gacctgcgcg acgccgagcc gtgggaggag gtgctccact acgagggcgg catcgaggcc
     1141 ttcgtcaaac atctggacaa ggccaagacc gcgctgatcg gcgccccgat cgccgtccgc
     1201 ggggtgcgcg agaaggtcga gatcgacctc gccctgcagt ggaacgacag ctaccacgag
     1261 cacatgctgt gcttcaccaa caacatcccg cagaaggatg gcggcacgca cctgtcggcc
     1321 ttccgcgcgg cgctgacgcg ggtgatcacc ggctatgccg agagctcggg catggccagg
     1381 aaggaaaagg tctcggtcac cggcgaggac gcgcgcgagg gcctgacctg cgtgctgtcg
     1441 gtcaaggtgc cggaccccaa gttcagctcc cagaccaagg acaagctggt ctcctccgag
     1501 gtccgcccgg cggtggaggg tctggtgcag gaggggctgg ccacctggtt cgaggaacat
     1561 ccggccgagg ccagggccat cgtgtcgaag atcgccgagg cggcctcggc ccgcgaagcc
     1621 gcccgcaagg cccgcgaact gacccggcgc aagagcgcgc tcgacatcac cagcctgccc
     1681 ggcaagctgg ccgactgcca ggaacgcgac ccggcgaagt ccgaactgtt catcgtcgag
     1741 ggtgactccg ccggcggctc ggccaagcag gcgcgcaacc gcgagaacca ggccgtgctg
     1801 cccctgcgcg gcaagatcct caatgtcgag cgggcccggt tcgacaagat gctgtcgtcc
     1861 gagcaggtcg gcaccctgat catggcgctc ggcgccggga tcggccgcga cgagttcgac
     1921 atcgccaaga tgcgctacca ccgcatcgtc atcatgaccg acgccgacgt cgacggggcg
     1981 catatccgca ccctgctgct gaccttcttc taccggcaga tgccggaggt gatcgagcgc
     2041 ggctacctct acatcgccca gccgccgctc tacaaagcct ccaagggcaa gtcctcgcgc
     2101 tatctgaagg acgacgccga gtacgaggcc ttcctgatcg acgagggcgt cgagggcgcc
     2161 gagctggacc tgtccagcgg cgagcggatg accggcaagg acctgctggc cctggtccag
     2221 accagccgcg cggccaaggc caacatcgag cggctgtcgg cccgcgcgcc gtcgttcgcc
     2281 atcgagcaga cggccctggc cggactgatg ggcgaacggc ccgatcccgc ggaagcggcc
     2341 cgccggctga acctctacgc ccaggagggc gacggcccgt ggagcggcga gcgcggcgag
     2401 accggctatg tgttcagccg ggtcaagcgc agcgtctcgg agaagatcgt gctcgaggac
     2461 gccctgctgc aggcggccga cgcccgccgt ctggccgagc gcgccgccct gctcgccgac
     2521 accttccagg gccaggccgt gttccgccgc aaggaccgga gtacgccggt gcgcggcccg
     2581 ctcgacctgt tcgcggccgt gatggacgcc ggtcgcaagg gtctgtcgat ccagcgctac
     2641 aagggcctgg gcgagatgaa ccccgagcag ctgtgggaga ccacgctcga ttccgaggcc
     2701 cgcacgctgc tgcaggtcaa ggtcaaccac gccgacgacg ccgacgacat gttcgcgcgg
     2761 ctgatgggcg aactggtcga gccgcgccgc gacttcatcc aggccaacgc cctcgacgcc
     2821 gaggtggacg tctagtcctt cgtcggcgcc tcggtcttca ggcgcttgac ggtcagctcg
     2881 tcgacctcga ggcgtttgac catgagggtc ttcaccttga gcctgccgat ggtcagccgg
     2941 ccgatggcca tgcgcgcgac ggtcagggcc ccgagcaggc ccatgccgat cgcggccatg
     3001 ccgagcgccg tggcgcccgt cgctgcggcg cccgaggccc gcaagccggt cgcgtccagc
     3061 cccgtcgcgg ttcgcgccgc cggggtcgtc tcgtggtcgt ggggctgatc ggtcatgcct
     3121 ggccctcctg tccggagggc atggaacagg ctggcgccgc gcggcgcctt gagcgggatc
     3181 aaaccgggcc ggtctagttg tccttgacca ccctgagcac cgccgcgccg tagcgcttca
     3241 gcttgctgtc gccgacgccg gtggccagca gcagttcgtc gggcgagcgg ggccgcagcc
     3301 ggacaatgtc ctgcagggtg cggtcgccga agatgacata gggcggcaca tgctgctcgt
     3361 gcgcctcgtc gcggcgccag gcgcgcaggg cctcgaacag ggccaggtcg tcgccggcga
     3421 atgtctccat cgcctctcgg gtccgcttgc gcggtcggcc agaccgggtc gagggatcca
     3481 ggccttccgg ctgccggcgc acggcgatgc gccgctcgcc gcggtagacc gccttgaccc
     3541 cttcgccgtc gcccagcccg atcagggggc ggccgtcgtt ggggtcctcc ctcagcaggc
     3601 cgtcaaacag cagctggtcg atcaggtcgc gccagccggc cggactgaac tccttgccga
     3661 cgccgtaggt cgacagggcc gcttcgctgt catgaacgtc tttcgtcttg cccaacaggt
     3721 ggtcgaccag ccggccgcgg ccgaagcggc cgccgagtcg gtggacggcg gccagggcct
     3781 tttgcgccgc caccgtggcg tcgaccgacc cgggcggggc caggcaaagg tcgcagacgc
     3841 cgcaagcctc gaccccctct tcgccgaaat aacggcgcac ggccgccgcg cggcaggaga
     3901 cgccgtcgag catggcgtag agctggcgca ccttgcgggt ctggacctgg cgcacctcct
     3961 cgctgacctc gcggccggcg atgcggcgca gggcccaggc catgtcggcc gagccgtaga
     4021 gggtgatgcc ctcggccggc aggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnggcg ggtcggcatg gatgacgaac cgcacgtccg gcttgtcgac
     4141 acccatgccg aaggcgatgg tcgccaccat caccgccgcg tcggcttcaa ggaaccgctc
     4201 cagccgctcc gtgcgctggg tgcgttcgag gccggcgtga taggccagcg ccggcacgcc
     4261 ggcggcgacc agccgttcgg ccaacctgtc cgtgccgtcc cgcgaaccgg tgtagaccac
     4321 gcccgaccgg ccgggacgcg ccagcaccag ttcctcgacc cggccgtggc cctgaccgcg
     4381 cttgcgctcg gccgacaacg ccagttccgg ccgggcgaag ctgtcgacga actcggcgca
     4441 gtcccgcagc cgcaactcgg tgcggatgtc gtcgcgcgtg cgggcgtctg cggtagccgt
     4501 caccgccagg cggggaacgt cgggaaacac ctcggccagc cggccgagca tgcggtattc
     4561 ggggcggaag tcgtggcccc actggctgac gcagtgggcc tcgtcgatcg cggcgatggc
     4621 cagcgggatg cgccgcagcc gctccagcgt cgcgcccagc atcagcccct ccggcgacag
     4681 gtagagcagg tccagctcgc cccgctcggc ccgacgccag acctccgccc gctcgtccgg
     4741 cgaatggctg gagtccagcc gctcggccgc cacgcccgac tgcttgaggc cttccacctg
     4801 gtcggccatc agggcgatca gcggcgagac caccaggccc aggcccggcc gcagcatggc
     4861 cgggatctgg tagcagaggc tcttgccgcc gcccgtcggc agcaccgcca tggcgctgcg
     4921 cccggccagg atctcggcga tcactgcggc ctgcagaccg cgaaactcgg catggccgaa
     4981 aacgcggcgc agaaggtcgc gggcgggggc gagggaatcg gtcaccgccc gcttatggcg
     5041 tctggtcgcg gccttgctaa ccccctgcgc gccgtctgca cggccgcgcc gcgaaaaggg
     5101 cgtgttcata tctgatcaat catggtggag ccttcgaggc tccggcgcta gaagtcggcg
     5161 ccaagggcac attcccggaa cagcgacggc gacatggcga accaacaggg cgtcaagcgg
     5221 acacccctac aggcgtttct ctactggggg gcggtggtcg gcgtctgggg gctgatcttc
     5281 ctggtcgcct tcttcgcggt gttcgccacc gacctgcctg acgtgtccaa gctgaacacc
     5341 gtcacccggc agccgtcgat ctcctacctc gaccggtccg gcgccctgat cgcggtgcgc
     5401 gggagccagt acgcgccgcc ggtcgatctc gacaagctgc cggactatgt gcccaaggcg
     5461 ttcatcgcca tcgaggaccg ccgcttctac taccaccccg gattcgaccc gatcggcatc
     5521 agccgggcga tcgtcaagga catcagctgc tggtgcgtcg cggccggcgg ctcgacgatc
     5581 acccagcagc tggcccgcaa cctgttcctg accctcgacc agacgccccg ccgcaaggcc
     5641 caggagctga tcctggcgat gtggctggag atgaagttca gcaagaagca gatcctcgcc
     5701 ctctacctga accgggtcta cttcggcgcc ggcgcctacg gcatcgaggc ggccgcgcag
     5761 cgctacttca acaagccggc ctcgcggctg accctgggcg aggcggcgct gctggccggg
     5821 atgatgaagg ggccttcgcg ctacagtccc gtttccgcta ccgaacgcgc cgcccgccgg
     5881 gcgaccatcg tgctcgacga gatggtccgc acccgggcga tcacccgcga gcagcgggcc
     5941 gaagcgttcc gcaatcccgt gcgggtctcg gcggtgctgg ccaaccagcg ggcgcagtat
     6001 ttcaccgact gggtcgacgc ccaggtgcgg tccctggtcg gcgagccgac ggaggacctg
     6061 atcgtcgaga ccaccctgga cctgccgatc caggccgccg ccgagtcggc ggtgcgctcg
     6121 ggcgtggtcg gggccaaggg ccagggtgtt cgccaggccg ccctggtggc catcgatggc
     6181 gagggtcgca tccgcgccta tgtcggcggc gccgactacg gcgagagcca gttcgaccgg
     6241 gccaccagcg cccgccgcca ggccggttcg gccttcaagc cgtttgtcta cctggccgcc
     6301 atggaggcgg gccgcacgcc agcggtgatg gtggtcgacg agccggtgcg tatcggcagc
     6361 tgggagccgc gcaactacac cggacgctac ctgggcgcca tcacattgca gacggccctg
     6421 gcccagtcgg tcaacaccgt cgcggcccga ctggccaacg aggtcggaac cggcaacgtc
     6481 gccgccgccg cccgtcggct gggcatcacc agcaagatcc agcttgaccc gtcgatggcg
     6541 ctgggcgccg tggaggtcaa tccgctcgag atggcgcagt cgtatgcggc cttttccaat
     6601 ggcggctttc tcgccaaggg ctacggcatc cagcgcatcc gcaccgccaa cgggcgcgtg
     6661 ctctatgacc gcaacctgga aaagcgcccg cgccggtcgg tgatcggctc gccggcgctg
     6721 cagtacatga accagatgat gcgcgaggtc gtgatcagcg gcaccggcaa gcgcgcgcga
     6781 gtgccgaact acgacatcgc cggcaagaca ggcaccacca gcgactaccg cgacgcctgg
     6841 ttcgtcggct acaccggcgg cttcgtcacc gccgtctgga ccggcaagga cgacaacacc
     6901 ccgatgaagg gagtcaccgg cggaggcgcg ccggcgggca tctggcgcgc cttcatggcg
     6961 caggccctgc cgcggctgaa ggcccaggcg atccccggcg gctatgcgcc ggtcgaggag
     7021 ccgcgcgacg ccatcggcga cattctctcg acgctcggca tgggcggaga ttccggcgcg
     7081 ccggccacgc cggctccgga cgcccgcact tcgccgcgcg tgccggagga gattccctac
     7141 tagaccgtca ggtccggccg acggcctgga gccgggcgcc ctcgcgcttc agccagcggc
     7201 ggtgcggcgc ggggtc
//
LOCUS       DAORNX010000105         7180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7723_length_7180_cov_6.820211, whole genome shotgun sequence.
ACCESSION   DAORNX010000105 DAORNX010000000
VERSION     DAORNX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_7180_cov_6.820211"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(765..4214)
                     /gene="dnaE"
                     /locus_tag="PLO65_06240"
     CDS             complement(765..4214)
                     /gene="dnaE"
                     /locus_tag="PLO65_06240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRD27880.1"
                     /translation="MAQDRAGGGADGGFVHLRVRSAYSLLEGAIKADAVGKMAADAGM
                     PAVAVTDRANLFGALEFSVTAKDAGVQPLVGCALPVTGIGEGRTERWARTPTIVLIAQ
                     NETGYLNLSALSSAAYLDSDGVAEPSVAWARVVEHAEGLILLSGGTDGPVDPLFAAGK
                     TRDGAKALAEMARVFGDRFYVELQRHGLPEQAAAEAGLVHWAYEHDTPLVATNDVYFA
                     KRDLYEAHDALLCISDGAFIGQDERRRVTAEHWFKPAADMRALFADLPEACDNSIDIA
                     RRCAFMVRKRDPILPGFPTTAGRDEGAELAHQAAEGLRNRLVGLELAAPEADYWARLE
                     REIGVIQAMGFPGYFLIVSDFIKWAKAQGIPVGPGRGSGAGSLVAWALTITDLDPLRF
                     GLLFERFLNPERVSMPDFDIDFCQERREEVIEYVQDKYGRGRVAQIITFGTLQARAVL
                     RDVGRVMQLPLGLVDRLAKMVPNNPANPVTLAQAINLEPRLRQARDEDPSVAQCLEVA
                     LRLEGLYRNASTHAAGVVIGDRPLVALTPLYKDPRSELPATQFNMKWVESAGLVKFDF
                     LGLKTLTVLDRAVKHLARRGAAIDLAKLPLDDAASYELMASGQTVGVFQLESQGMRDT
                     LRKMRPGTIEEITALISLYRPGPMDNIDLFIDTKFGRRPMDTMHPSLEEVLKETYGVI
                     VYQEQVMRIAQVLAGYSLGEADLLRRAMGKKKKEEMDQQKARFVSGAIARGVDGDKAG
                     AIFEHVEKFAGYGFNKSHAAAYALIAYQTAWLKANAPVEFLAGSMTLDLSNTDKLAVF
                     YQDARRFGITVRPPDINRSGADFEVEDGAVLYALGAVRNVGFAAMEHVVAVRAEGGPF
                     RDLFDFVERVDPRQVNRRALESLARAGAFDAIHPNRAQIVEAADVLIAHGQSLAADRA
                     SAQVSLFGGEQPDAARPRLPRTDPWDQIRRLDEELAAVGFYLTGHPLEDMVEVLRRRR
                     TTMLSDAIPQAEAGTEAFRMAGMVRRRQERAQQSGERFAFVSLSDPTGEYEVLFPPEA
                     LRKCRDILEPGKAVAIKVRAKARDGEVRFFGDDAEPIDKAIESVAASLRVHVSPQSME
                     IEALKKRLSVASAPRGGEVVLVAGLGAGREVEVKLPGFYNLDASLRGALKTAPGVAYL
                     EDV"
     gene            complement(4318..4407)
                     /gene="sidA"
                     /locus_tag="PLO65_06245"
     CDS             complement(4318..4407)
                     /gene="sidA"
                     /locus_tag="PLO65_06245"
                     /inference="COORDINATES: protein motif:HMM:NF033228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SidA"
                     /protein_id="HRD27881.1"
                     /translation="MVRIARESLALVSVAGFVWMMCSVAYAVA"
     gene            4492..4917
                     /locus_tag="PLO65_06250"
     CDS             4492..4917
                     /locus_tag="PLO65_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27882.1"
                     /translation="MDQRLSLITLGVADLAVARAFYGGGLGWTPLDPLPDVVFYQLPG
                     LALALFGREDLARDVGEPLPGPGGAFTLAINGRSPAEVDRTFARAVAAGARAVKPPRT
                     AAWGGYSGYFADPDGHLWETAYNPGCEITPRGETFFGGR"
     gene            complement(4880..5236)
                     /locus_tag="PLO65_06255"
     CDS             complement(4880..5236)
                     /locus_tag="PLO65_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27883.1"
                     /translation="MTRQIDFAGQSGVLYRYKALEEMRPVPPGGANFVYVKWDGDRPV
                     IVYAGETDSLHRSVFETWDEARSGYGATEIFARLNITGVVRRSEYDDLVAQHQPPMNT
                     GGESLSGRRKTSRPGA"
     gene            complement(5330..6022)
                     /locus_tag="PLO65_06260"
     CDS             complement(5330..6022)
                     /locus_tag="PLO65_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD27884.1"
                     /translation="MSEPVLSLRGVTRTYVTDAGELPVLKGVDLDVMPGEIVGLIGPS
                     GSGKSSLLHAAGLLERPDGGTVSVAGQDCTRLGDKPRTRMRLGTIGFVYQFHHLLPEF
                     TALENVALPAMIAGRSRAFARDRAETLLTRLGLAERLEHQPAQLSGGEQQRVAIARAL
                     TNEPRLLLADEPTGNLDPDTAASVFQALYDLARDTGVAALIATHNMELARYMDRVFAL
                     RDGHLEERTPGF"
     gene            complement(6015..>7180)
                     /locus_tag="PLO65_06265"
     CDS             complement(6015..>7180)
                     /locus_tag="PLO65_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRD27885.1"
                     /translation="IGITLAVTVLIVVMSVMNGFRTELLDRILGFNGHAYVTGAPLGS
                     PDRAAIVKRLKAVPGVRQVIPTVEGQAMVLGPSQIAGAIVRGVTPADLQATTLVSSNI
                     KPGSAEGFGQGDYGGELILVGERLAASLGVGPGDSVALISPTGAATAFGTSVTQKDYT
                     VGGIFSVGMSEYDQAFIYMPLQQAQLFFGRDDTVDAIEIMLDDPDSVAAIKPLLAAEA
                     GPGAIVTDWTQKNQSFFEALQVERAAMRLILMFIVAIAAMNIISGLVMLVKNKGRDIG
                     ILRTMGAGQGAILRIFFMSGAAVGVAGTVAGLLLGVVICTNIEAIQKAVEWATGANVF
                     NADVYFLTHVPAKIEWREVGIITGWSLFMSFVATLPPAWRASRIDPVEALRYE"
BASE COUNT         1114 a         2487 c         2458 g         1121 t
ORIGIN      
        1 agggagtagg gagtagggag cgcccttccc tgcccgttca gcagtccggc cttcggcaga
       61 aaggcgggaa agcccttacg accttgccga gattggagct ccgttgaccg gatgggtcaa
      121 ggacggcccc agcggggccg ccgctgcgcg gccgcgacgc ggtccttgag ccatccggtc
      181 aacggagcag actggcctgc aagacgtaag gggcggtgtg gtgcgggcca aaacgtccgc
      241 tcatccgcgc gaaagcgatg acccagggca tcgctgcctg cctagatgag cggttctcaa
      301 ggtgtgaagc agacctcgcg aagttccgcg ttgggtggga aacggacgcc tacttcttcg
      361 acttgacgac cttgatcaca aggtcgcaag gcgtgtcgga gagtgtgcat tgccacgctt
      421 cggccattcc gattactcgt tgaagccgaa ggcggattag gtcggtccca cgccgagcgg
      481 acacttcggg aaatgcctcc ctcactgcct cactgagcca cccctgcgga ttgctcctgc
      541 gaagctcggt ggttcgcgtc tccgatccga ccgtcatggt gtaggttaat agcgacatgc
      601 aagtcagcgc tgcgcgctgg cagttgttcg tcaacgtccg ctaagggtcg ataacgcact
      661 tcggcagttc acttcggcgg gcaagcgcca tttgaaccat gggcggatgt cccctgaaaa
      721 agcctgggtc ccggccttcg ccgggatgag cggaaatgga gagcctacac gtcctccagg
      781 tacgccaccc ccggcgcggt cttcagcgcg cctctcagtg acgcgtcgag attgtagaac
      841 cccggcagtt tgacctcgac ctcgcggccg gcgccgaggc ccgcgaccag cacaacctcc
      901 ccgccgcgcg gagccgaggc gaccgacagg cgcttcttca gcgcctcgat ctccatcgac
      961 tggggggaga catggacgcg caggctggcg gcgaccgact cgatggcctt gtcgatcggc
     1021 tcggcgtcgt cgccgaagaa gcgcacctcg ccgtcgcggg ccttggcccg caccttgatg
     1081 gccacggcct tgcccggttc gaggatgtcg cggcacttgc gcagggcctc cggcggaaac
     1141 agcacctcgt actcaccggt cggatcggac agcgagacaa aggcgaaccg ctcgccgctc
     1201 tgctgcgccc gctcctgccg gcgccggacc atgccggcca tgcggaaggc ctccgtcccg
     1261 gcctcggcct ggggaatggc gtcgctgagc atggtcgtgc gccgtcggcg cagcacctcg
     1321 accatgtcct cgagcggatg gccggtcagg tagaagccaa ccgccgcaag ttcctcgtcg
     1381 agtcggcgga tctggtccca cggatcggtg cgcggcagcc gcgggcgggc cgcgtcgggc
     1441 tgctcgccgc cgaacaggct gacctgggcg ctggcgcggt cggcggccag gctctggccg
     1501 tgggcgatga gcacgtcggc ggcctcgacg atctgggccc ggttggggtg gatggcgtcg
     1561 aaggccccgg cgcgggccag gctttccagc gcccgccggt tgacctgccg cggatcgacc
     1621 cgctcgacga agtcgaacag gtcgcggaac ggcccaccct cggcccgcac cgccaccaca
     1681 tgctccatgg ccgcgaagcc gacgttgcgc accgctccca gggcgtagag cacggccccg
     1741 tcctcgacct cgaagtccgc gcccgaccgg ttgatgtcgg gcggacggac ggtgatgccg
     1801 aacctgcgcg cgtcctggta gaacaccgcc agcttgtcgg tgttgctgag gtccagggtc
     1861 atcgagccgg cgaggaactc gaccggggcg ttcgccttca gccaggccgt ctggtaggcg
     1921 atcagcgcgt aggcggcggc gtgcgacttg ttgaagccgt agccggcgaa cttctcgaca
     1981 tgctcgaaga tggccccggc cttgtcgccg tcgaccccgc gcgcgatggc cccggagacg
     2041 aaccgcgcct tctgctggtc catctcctcc ttcttcttct tgcccatcgc ccgccgcagc
     2101 aggtcggcct cgcccaggct gtagccggcc aggacctggg cgatgcgcat cacctgttcc
     2161 tggtagacga tgacgccgta ggtctccttg agcacctcct ccagcgacgg atgcatggtg
     2221 tccatcgggc ggcgaccgaa cttcgtgtcg atgaacaggt cgatgttgtc catcgggccc
     2281 ggccggtaga gcgagatcag ggcggtgatc tcctcgatgg tgccggggcg catcttgcgc
     2341 agggtgtcgc gcatgccctg gctttccagc tggaacaccc cgacggtctg gcccgaggcc
     2401 atcagctcgt aggacgccgc atcgtccagc ggcagcttcg ccaggtcgat ggccgcgccg
     2461 cgccgggcca ggtgcttgac cgcccggtcg agcacggtga gcgtcttgag accgaggaag
     2521 tcgaacttca caaggcccgc ggactcgacc catttcatgt tgaactgggt cgccggcagc
     2581 tccgagcgcg ggtccttgta gaggggggtc agcgcgacca gcggccggtc gccgatgacc
     2641 accccggcgg cgtgggtcga ggcgttgcgg tagaggccct cgagccgcag ggcgacttcc
     2701 aggcactggg cgaccgacgg gtcctcgtcg cgggcctggc gcaaacgcgg ctcgagattg
     2761 atcgcctggg ccagggtcac cgggttggcc gggttgttgg gcaccatctt ggccaggcgg
     2821 tcgaccaggc ccagcggcag ctgcatcacc cgcccgacgt cgcgcagcac ggcgcgggcc
     2881 tgcagggtgc cgaaggtgat gatctgggcg acgcggccgc ggccgtactt gtcctgcacg
     2941 tattcgatca cctcctcccg ccgctcctgg cagaagtcga tatcgaagtc gggcatcgag
     3001 acccgctcgg gattgaggaa gcgttcaaac agcaggccga accgcagcgg gtccaggtcg
     3061 gtaatggtca gggcccaggc gaccagcgaa ccggcgcccg agccgcggcc cggaccgacc
     3121 gggatgccct gggccttggc ccatttgatg aagtccgaca cgatcaggaa gtaaccggga
     3181 aaacccatcg cctggatcac gccgatctcg cgctcgagac gggcccagta gtcggcctcc
     3241 ggcgcggcca gctccagccc gacgaggcgg ttgcgcagcc cctccgcggc ctggtgggcc
     3301 agctcggctc cctcgtcacg gccggccgtg gtcgggaagc cgggcaggat cgggtcgcgc
     3361 ttgcgaacca tgaaggcgca gcgccgggcg atgtcgatgc tgttgtcgca ggcctccggc
     3421 aggtcggcga acagcgcccg catgtccgcc gccggcttga accagtgctc ggcggtcacc
     3481 cgccgccgct cgtcctggcc gatgaaggcg ccgtcggaaa tgcacagcag ggcgtcgtgc
     3541 gcctcgtaca ggtcccgctt ggcgaaatag acatcgttgg tcgcgaccag cggcgtgtcg
     3601 tgctcatagg cccagtgaac cagcccggcc tcggccgccg cctgctcggg caggccgtgg
     3661 cgctgcagct cgacatagaa ccggtcgccg aacacccggg ccatctcggc cagggccttc
     3721 gccccgtccc gcgtctttcc cgcggcgaac agcgggtcga ccggcccgtc ggtcccgccg
     3781 ctgagcagga tcaggccctc ggcgtgctcg acgaccctgg cccaggccac gctcggctcg
     3841 gccacgccgt cgctgtcgag gtaggccgcc gacgacaggg ccgagagatt gagatagccg
     3901 gtttcgttct gggcgatcag cacgatcgtc ggcgtccgcg cccagcgctc ggtccgcccc
     3961 tcgccgatgc cggtcaccgg cagggcgcag ccgaccagcg gctggacccc ggcgtccttg
     4021 gcggtgaccg aaaactcgag agcgccgaac agattggccc ggtccgtcac ggccaccgcc
     4081 ggcatgccgg cgtcggcggc catcttgccg acggcgtccg ccttgatcgc cccttcaagc
     4141 agcgaatagg ccgaccggac ccgcagatga acgaatccgc cgtcggcgcc gccccctgcc
     4201 ctgtcctgcg ccatcgcgct gtcccttcag atcatcccgt ccgaccacac cggccagcca
     4261 acgaacgagt caccgcagcg accatcgccc gcccgacgct tccgatccag cgccgactta
     4321 ggctacggcg taggcgaccg aacacatcat ccacacaaat cccgccacgg agacgagagc
     4381 cagcgattca cgcgcgatcc ggaccattcg ggcctcctcg gaacaagttg cctgtttgtt
     4441 cttattcctg ttttgttctc actcgtcaac cgcaattcgg agatcgtcgt catggaccag
     4501 cgcctgagcc tgatcaccct gggcgtggcc gacctggccg tcgcccgggc cttctacggg
     4561 ggcggcctcg gctggacacc gctcgacccc ctgcccgacg tggtgttcta ccagctgccc
     4621 ggcctggccc tggccctgtt cggccgggaa gacctggcgc gggatgtcgg cgagcccctg
     4681 cccgggccgg gcggagcctt caccctggcg atcaacggcc gcagtccggc cgaggtcgac
     4741 cggaccttcg cccgggccgt cgccgccggc gcccgcgcgg tcaagccgcc gcggacggcg
     4801 gcctggggcg gctactcagg ctatttcgcc gatccggacg gccatttgtg ggagaccgcc
     4861 tacaaccccg gatgcgagat cacgccccgg ggcgagacgt ttttcggcgg ccgctgaggc
     4921 tctcgccgcc ggtgttcatc ggcggctgat gctgggccac caggtcgtcg tactcgctgc
     4981 gccgcacaac gccggtgata ttcagccggg cgaagatctc ggtcgcgccg tagcccgacc
     5041 gggcctcatc ccaggtctcg aacacgctgc gatggaggct gtcggtctcg ccggcgtaga
     5101 cgatcaccgg ccggtcgccg tcccatttca catagacgaa gttggccccg cccggcggga
     5161 ccggacgcat ctcctcgagc gccttgtagc gatagaggac cccggactgg ccggcgaaat
     5221 cgatctggcg ggtcaaacgc acgctcccct gccgctgcac atggaccgcc ttaaggcccg
     5281 tcgatctggg cgaaaacgcg gcgctgacga agccgtgatc gcggccgccc tagaaccccg
     5341 gcgtccgctc ttccaggtga ccgtccctca gggcgaagac gcggtccatg tagcgggcca
     5401 gctccatgtt gtgcgttgcg atcagggccg ccacacccgt gtcgcgggcc agatcgtaga
     5461 gggcctggaa caccgaggcg gcggtgtccg ggtcgaggtt gccggtcggc tcgtcggcca
     5521 gcagcagccg gggctcgttg gtcagggcgc gggcgatggc cacccgctgc tgttccccgc
     5581 ccgacagctg ggcaggctgg tgttccagcc gctcggccag gcccaggcgc gtcagaagcg
     5641 tctcggcccg gtcgcgggcg aaggcgcggc tgcgaccggc gatcatcgcc ggcagggcga
     5701 cgttctccag cgcggtgaac tccggcagca ggtggtgaaa ctggtagacg aagccgatcg
     5761 tgccgagccg catgcgggtg cgcggcttgt cgcccagccg ggtgcagtcc tggccggcga
     5821 cggagaccgt gccgccgtcc ggtcgctcga gcaggccggc ggcgtggagc agcgacgact
     5881 tgcccgagcc ggacgggccg atcagcccga cgatctcgcc gggcatgacg tccaggtcga
     5941 cgcccttgag caccggcagt tcgccggcgt cggtgacata ggtgcgggtc acccccctca
     6001 gggacaggac aggctcactc atagcgcagg gcctcgaccg gatcgatgcg cgaggcccgc
     6061 caggccggcg gcagggtggc gacaaagctc atgaacagcg accagccggt gatgatgccg
     6121 acctcgcgcc attcgatctt ggccggaacg tgggtcagga aatagacgtc ggcattgaac
     6181 acattggccc cggtggccca ttcgacggcc ttctggatcg cctcgatgtt ggtgcagatg
     6241 accacgccca gcagcaggcc ggcgaccgtg ccggctacgc cgaccgccgc gcccgacatg
     6301 aagaagatgc gcaggatggc gccctgtccc gcgcccatgg tgcgcaggat gccgatgtcg
     6361 cgccccttgt tcttcaccag catgaccagg ccggaaatga tgttcatcgc ggcgatggcg
     6421 acgatgaaca tcaggatcag ccgcatggcc gcccgttcga cctgcagggc ctcgaagaag
     6481 ctctggttct tctgggtcca gtcggtgacg atcgcgcccg ggccggcctc ggccgccagc
     6541 agcggcttga tcgcggcgac gctgtcgggg tcgtcgagca tgatctcgat ggcgtcgacg
     6601 gtatcgtcgc ggccgaagaa cagctgggcc tgctgcaacg gcatgtagat gaaggcctgg
     6661 tcgtactcgc tcatgccgac cgaaaagatg ccgccgaccg tgtagtcctt ctgggtcacg
     6721 ctggtgccga aggccgtggc cgcgccggtc ggcgaaatca gggccacgga gtcgccgggc
     6781 ccgacgccga ggctggcggc cagccgctcg ccgacgagga tcagctcgcc gccgtagtca
     6841 ccctggccga agccctcggc cgagcccggc ttgatgttgc tggacaccag ggtggtggcc
     6901 tggaggtcgg ccggggtgac gccgcgcacg atggcgccgg cgatctggct cgggcccagc
     6961 accatggcct ggccctcgac ggtcgggatc acctgtcgca cgccgggcac ggccttgagc
     7021 cgtttgacaa tggcggctcg gtcgggactg ccgagcggag ccccggtgac gtaggcatgg
     7081 ccattgaagc cgaggatgcg gtcgagcagc tcggtgcgga agccgttcat cacgctcatc
     7141 accacgatca gcaccgtcac cgccagggtg atgccgatga
//
LOCUS       DAORNX010000106         7179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7724_length_7179_cov_6.072572, whole genome shotgun sequence.
ACCESSION   DAORNX010000106 DAORNX010000000
VERSION     DAORNX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_7179_cov_6.072572"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2515)
                     /locus_tag="PLO65_06270"
     CDS             complement(29..2515)
                     /locus_tag="PLO65_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD27886.1"
                     /translation="MTIVAKPLARKLLAIVVVLSIAVTGLATAATFAFVQKASMQRQI
                     GNLQLYVKERTATEDRLFSDLVKVHADATDALQRRLGRINGPSLDARFDRLFPLRPDG
                     TRRSAPGLFDGETRGDGTYMYGMGAFLADGARISPQQKALFIAALQVVSLAGEAQHTR
                     YDNFYMFTPDNRLVMYGPDRDDRLIYYRQDAPATLDFRNEEMTELTRPRANPARVMKC
                     TKLRKLMSDPTGKALTTACMTPIDIDGVHVGAWGTTVTLDSYLMAAIGESIQGGENMI
                     VSADGELIAAPGLGKGGIVSPSELVREQQRRGIAPLVRRIRAEGGGSGVFQSGERVIA
                     YGHLKEPDWYFVMSLPTSEIAWSAARTASWVLLFGLIGIVVQALLLYRFIHREVVDPL
                     VTLSVAHARDGSARAPQVEARQDEIGGLARMLRGQRVDNDELFRSLEDRVAARTAELE
                     RANRAKSTFLANMSHELRTPLNGVIALTDQLAETADQTRRRELAGLVSSSGRLLEQVL
                     SDILDVSKIEAGQFRLSPAPFDLTGTVAAMADLHRASAEAKGLTLTWSIAEAARGVWL
                     GDSGRIAQILSNLLGNAVKFTDRGEVALSVDRHDGGLRFTVRDTGVGFDAEARERLFR
                     RFEQADDSITRRFGGTGLGLSICSALTGMMGGQIDATATPGEGAVFTVDLPLPRAELA
                     APETGAQPEVEVSLQGLRVLAAEDHPTNQKVLQIVLEPFGVDLTIVGNGEEALAAFGD
                     GYDAVLMDMQMPVMDGLTATRLIREREAASGAEPALIIMLTANAMDDHVEAARLAGAD
                     LHVAKPLRPAQLFAALARARQPREAVSAAA"
     gene            2712..3926
                     /locus_tag="PLO65_06275"
     CDS             2712..3926
                     /locus_tag="PLO65_06275"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRD27887.1"
                     /translation="MAKIKVANPVVDMDGDEMTRIIWKLIKDKLIFPYLDLELDYYDL
                     SIEHRDATNDQVTIDAAEATKRHGVAVKCATITPDEQRVEEFKLKKMWKSPNGTIRNI
                     LGGVIFREPIICQNVPRLVPGWTQPIIVGRHAFGDQYRATDFRFPGKGKLTIKFVGED
                     GKVIEHEVFDAPGSGVAMAMYNLDDSIRDFAHASFAYGLARKYPVYLSTKNTILKAYD
                     GRFKDIFQEVFDAEYAGKFKELGLTYEHRLIDDMVASALKWSGGYVWACKNYDGDVQS
                     DIVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYRQHQKGESTSTNSIASIFAW
                     TRGLEHRGKLDGNAELIRFAQTLEKVCVDTVESGFMTKDLALLVGDQQSWLTTEGFLD
                     KVDENLKAAMAA"
     gene            3927..4370
                     /locus_tag="PLO65_06280"
     CDS             3927..4370
                     /locus_tag="PLO65_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27888.1"
                     /translation="MAFTGTIRPLAPSDRTAWDGLWTGYLTFYKSSVAPEVTEATWAR
                     LMDPAAPMFALVAEDAAGVFGLVQCVLHASSWSTQPYCYLGDLFVDPGRRGTGAGRAL
                     IEAVYAEADRHGAARVYWNTHETNATARRLYDQVATRSGFIQYRR"
     gene            complement(4396..4779)
                     /locus_tag="PLO65_06285"
     CDS             complement(4396..4779)
                     /locus_tag="PLO65_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27889.1"
                     /translation="MIGYVTLGALDVEKSLPFWDAVFGAIGGERGFFSGGWAGYGPAG
                     GPQTVYICPPHDGQPARGGNGVMVALLAPSTDAVKAAHAAGLAQGGTDEGAPGPRPED
                     SKDFYGAYLRDAAGNKICVYAKGPF"
     gene            complement(5084..5650)
                     /locus_tag="PLO65_06290"
     CDS             complement(5084..5650)
                     /locus_tag="PLO65_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HRD27890.1"
                     /translation="MRFDLFKIVATAALIFALAFASAPWFAFRALRAATAAEDVQATA
                     ELVDFPAVRKSLTGQLAPAPATTSAEPPSIWQDPLGALKRAIEPLAPPEPKVDRYLTL
                     PGLSALTRGYQPGKAPPPANGVQPLEVKLKAAMTGPWPTVPYWDPNRARIAVRRPGQE
                     DKVTVFTFERRALFTWKLVHVQLPPGER"
     gene            complement(5898..6980)
                     /locus_tag="PLO65_06295"
     CDS             complement(5898..6980)
                     /locus_tag="PLO65_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HRD27891.1"
                     /translation="MRVTIGMAAALMAISSPATAREYRPAFHPDQLKAPATRAPNEVL
                     VLGTPHLSGLPDSFRPEQLEPLLDRLAAWRPTAISTENLSGLQCDSLRRYAYRYASTV
                     PDYCYDPTAAGLAAGLDVPAANAEVERLFAHWPDAPSPGERRRMALLLLAAGEPGSAV
                     VQWLRLPEAERLARDGLTEALVQALNRRVTRRNETELVAAALAARLGLERVWSVDDQS
                     AASETPPEDSDAFGQALMGAWDNPASKARKAADERLYAGLGEADGVLNLYRAFNDPAA
                     AMVVYQSDFGAALAEPSPGQFGRRYVGYWETRNLRMVANIRDVLGRYPGTRMLTIVGA
                     SHRGYYEAYLNLMHDVVLVDAATVLK"
BASE COUNT         1113 a         2537 c         2474 g         1055 t
ORIGIN      
        1 gggtgatcac gcgagcggcc ggctaggctc aggcggccgc gctgacggcc tcgcggggct
       61 gacgcgcccg ggcgagcgcg gcgaacagct gggccggcct cagcggcttg gccacgtgca
      121 ggtccgcccc ggccagcctg gcggcctcga catggtcgtc catcgcattg gcggtcagca
      181 tgatgatcag ggccggctcg gcgcccgagg ccgcctctcg ctcgcggatc aggcgggtag
      241 cggtcaggcc gtccatgacc ggcatctgca tgtccatcag caccgcgtca tagccgtcgc
      301 cgaacgccgc cagggcctcc tcgccgttgc cgacgatggt caggtcgacg ccgaacggct
      361 cgagcacgat ctgcagcacc ttctggttgg tcggatgatc ctcggccgcc agcacccgca
      421 ggccctgcag gctgacttcg acctcgggct gggcgcccgt ttcgggcgcg gccagctcgg
      481 cgcgcggcag cggcaggtcg acggtgaaca ccgcgccctc gcctggcgtc gccgtggcgt
      541 cgatctggcc gcccatcatg cctgtcaggg ccgaacagat cgacaggccc agccccgtgc
      601 cgccgaaccg gcgggtgatc gaatcgtcgg cctgttcgaa ccggcggaac agccgttcgc
      661 gcgcctcggc gtcgaaaccg actcccgtgt cgcggaccgt gaatcgcaga cccccgtcgt
      721 ggcggtcgac gctcagcgcc acctcgcccc ggtcggtgaa cttcaccgcg ttgccgagca
      781 ggttgctgag gatctgggcg atccggccgc tgtcgccgag ccagacgccg cgcgccgcct
      841 cggcgatcga ccaggtcagg gtcagcccct tggcctcggc cgaggcgcgg tgcagatcgg
      901 ccatcgccgc gaccgtgccg gtcaggtcga acggcgcggg cgacaggcgg aactgcccgg
      961 cctcgatctt cgacacgtcg aggatgtcgc tgagcacctg ttccagcagc cgcccggatg
     1021 aggagaccag gccggccagc tcgcgccgcc gcgtctggtc ggccgtctcc gccagctggt
     1081 cggtcagggc gatgacgccg ttcagcggcg tgcgcagctc gtgcgacatg ttggccagga
     1141 aggtcgactt ggcccggttg gcccgttcca gctccgccgt gcgggccgcg acgcgatcct
     1201 cgagcgagcg gaacagttcg tcgttgtcga cccgctggcc gcgcagcatg cgggcgaggc
     1261 cgccgatctc gtcctgccgc gcctcgacct ggggggccct ggcagagccg tcgcgggcgt
     1321 gcgcgaccga cagggtgacc agcggatcga ccacctcgcg gtggatgaac cggtagagca
     1381 gcagcgcctg gacgacgatg ccgatcaggc cgaacagcag cacccaggac gctgtccggg
     1441 ccgccgacca ggcgatctcc gacgtcggca gactcatcac gaaataccag tccggctcct
     1501 tgaggtggcc gtaggcgatc acccgttcgc cggactggaa cacgcccgat ccgccgccct
     1561 cggcgcggat ccgccggacc aggggcgcaa tgccgcggcg ctgctgctcg cgcaccagtt
     1621 ccgacggcga gacgatgccg cccttgccca ggcccggcgc ggcgatcagt tcgccgtcgg
     1681 cggagacgat catgttctcg ccgccctgga tcgactcgcc gatggcggcc atcaggtagg
     1741 agtccagcgt cacggtcgtg ccccaggccc cgacatggac cccgtcgatg tcgatcggcg
     1801 tcatgcaggc ggtggtcagg gccttgccgg tcgggtcgct catcagcttg cgcagcttgg
     1861 tgcatttcat cacccgcgcc ggattggccc ggggacgggt cagctcggtc atctcctcgt
     1921 tgcggaagtc gagcgtcgcc ggggcgtcct gacgatagta gatcagccgg tcgtcgcggt
     1981 cggggccgta catcaccagc ctgttgtcag gcgtgaacat gtagaagttg tcatagcgcg
     2041 tatgctgcgc ctcgccggcc agggacacga cctgcagcgc agcgatgaac agggccttct
     2101 gctgcggact gatccgcgcg ccatcggcga ggaaggcgcc catgccgtac atgtaggtgc
     2161 cgtcgccgcg ggtctcgccg tcgaacaggc ccggcgcgct gcgccgcgtt ccgtccggcc
     2221 tcaggggaaa cagccggtcg aagcgcgcgt cgagcgaagg cccgttgatc cgccccagac
     2281 gtcgctggag cgcatcggtc gcatcggcgt gaaccttgac caggtcagag aacagccggt
     2341 cctcggtggc ggtgcgctcc ttcacgtaga gctgcagatt gccgatctga cgctgcatcg
     2401 aggccttctg cacaaaggcg aaggtcgcgg cggtggccag acccgtcacg gcgatggaca
     2461 gcaccaccac gatggccagc agcttccgcg ccagcggttt ggcgacaatc gtcatggagc
     2521 caacggccct cccctcgatg ggacaggctt aacggccaga cctgaacagg ccgttgactg
     2581 catcgcgcgg ccgccgctgc cgccttcccc cgtcgcccgg caggcgctat agccctcacc
     2641 gcccgcgctc gcggcggccc tgtgcgccgc cgctttcccc gcagaagcgc gcccaacgga
     2701 gagacattcc catggccaag atcaaggtcg ccaatccggt cgtcgacatg gacggcgacg
     2761 agatgacccg catcatctgg aagctcatca aggacaagct gatcttcccc tacctcgacc
     2821 ttgagcttga ctactacgac ttgtcgatcg agcaccgcga cgccaccaac gaccaggtga
     2881 ccatcgacgc ggccgaggcc accaagcgcc acggcgtggc cgtcaagtgc gcgaccatca
     2941 cccccgacga gcagcgggtc gaggaattca agctcaagaa gatgtggaag agccccaacg
     3001 gcacgatccg caacatcctg ggcggggtga tcttccgcga gccgatcatc tgccagaacg
     3061 tgccccgcct ggtgccgggc tggacccagc cgatcatcgt cggccgccac gctttcggcg
     3121 accagtaccg tgcgacggac ttcaggttcc cgggcaaggg caagctgacg atcaagttcg
     3181 tcggcgagga cggcaaggtc atcgagcacg aggtgttcga cgccccgggc agcggcgtgg
     3241 ccatggccat gtacaacctc gacgactcga tccgcgactt cgcgcacgcc tcgttcgcct
     3301 acggcctcgc ccgcaaatac ccggtctacc tgtcgaccaa gaacaccatc ctcaaggcct
     3361 acgacgggcg cttcaaggac atcttccagg aggtgttcga cgcggaatac gccggcaagt
     3421 tcaaggaact gggcctgacc tacgagcacc ggctgatcga cgacatggtc gccagcgcgt
     3481 tgaagtggtc cggcggctat gtctgggcct gcaagaacta cgacggcgat gtgcagagcg
     3541 acatcgtcgc ccagggcttc ggctcgctgg gcctgatgac ctcggtgctg gtcacgccgg
     3601 acggcaagac catggaggcg gaagccgccc atggcaccgt cacccgccac taccgccagc
     3661 accagaaggg cgagtcgacc tcgaccaact cgatcgcctc gatcttcgcc tggacccggg
     3721 gcctcgagca ccgcggcaag ctcgacggca acgccgagct gatccggttc gcccagaccc
     3781 tggaaaaggt ctgcgtcgac accgtcgagt ccggcttcat gaccaaggac ctggccctgc
     3841 tggtcggcga ccagcagagc tggctgacca ccgaagggtt cctcgacaag gtcgacgaga
     3901 acctcaaggc ggcgatggcg gcctgattgg ccttcaccgg gaccatccgc ccgctcgcgc
     3961 cgtccgaccg gacggcgtgg gacgggctgt ggaccggcta cctgaccttc tataagtcga
     4021 gcgtcgctcc ggaggtgacc gaggcgacct gggcgcggct gatggatccc gccgcgccga
     4081 tgttcgccct ggtggccgaa gacgccgcgg gggtgttcgg cctggtccag tgcgtgctgc
     4141 acgcctcgag ctggtcgacg cagccctact gctatctcgg cgatctgttc gtcgaccccg
     4201 gtcgccgcgg gaccggcgcc ggccgggccc tgatcgaggc ggtctacgcc gaggccgacc
     4261 ggcacggcgc ggcccgggtc tactggaaca cgcacgagac caacgccacg gcccgccggc
     4321 tctacgacca ggtggcgacg cggagcggtt tcatccagta ccggcgctga cccgcgcgcg
     4381 gccgtgacgg ccgcctcaga acggtccctt ggcgtagacg cagatcttgt tgccggcggc
     4441 gtcccgcaga taggcgccgt agaagtcctt cgagtcttct ggccgcggcc ccggagcccc
     4501 ctcgtcggtc ccgccctggg ccaggcccgc ggcgtgggcg gccttcacgg cgtcggtcga
     4561 gggggccagc agggcgacca tgaccccgtt gccgccccgg gccggctggc cgtcgtgtgg
     4621 cgggcagatg tagacggtct gcggtccgcc cgcggggccg taaccggccc agccgccgct
     4681 gaagaagccg cgctcgccgc cgatggcgcc gaacaccgcg tcccagaagg gcagcgattt
     4741 ctcgacgtcg agcgcgccga gggtgacata tccgatcatg gtggtctctc ccgtttcgtg
     4801 gaccgacgtt accgtggcgg ttcgaacaaa acaagaacgc gcggccgatg gagagctggc
     4861 tgctgacagg acggtggcgg gagggggccg gggagtaggg agtagggagt agggagtagc
     4921 aagaggggcc aggcgccagg gaccagggac cagggactag gggaatggcg gccggtcagg
     4981 ccgggcgccc gccgcgacca agcccgacta ccccctactc cctactccct attccctact
     5041 ccctactccc tagcccctac tccctagccc ctggctactc cccctaccgc tcccccggcg
     5101 gcagttgcac atgcaccagc ttccaggtga acagggcccg gcgctcgaag gtgaacacgg
     5161 tgaccttgtc ctcctgcccc ggccgccgga cggcgatgcg ggcgcggttg ggatcccagt
     5221 agggcacggt cggccagggg cccgtcatcg ccgccttgag cttgacctcc agcggctgga
     5281 cgccgttggc cggcggcggc gccttgccgg gctggtagcc gcgggtcagg gccgacaggc
     5341 ccggcagggt caggtagcgg tcgaccttcg gctcgggcgg ggccagcggc tcgatggctc
     5401 gcttcagcgc gccgagcgga tcctgccaga tcgatggcgg ctcggccgag gtggttgccg
     5461 gggccggcgc cagctggccg gtcaggctct tgcgcacggc cgggaagtcg accagctcgg
     5521 ccgtcgcctg cacatcctcg gccgccgtcg ccgcccgcag cgcgcggaac gcgaaccagg
     5581 gcgcgctggc gaaggccagg gcgaagatca gcgccgccgt ggcgacaatc ttgaagaggt
     5641 cgaaccgcat ggggctgcac cgttaaccgt ggggaaccat caaagcacgg tccgggggcc
     5701 gtttggcaag ccgcgctcgc ccgtggcggg aacgacgtgg ccggccagaa gtccccggat
     5761 gtcgcgacat gtcccgggat tgccggggtc gggagtctgg ctcgcgagcc tctccccggc
     5821 ctttcctcca tcgcggagac cggtaacccc gatcgcgccc ggccatggcg ggcgagaggc
     5881 cggaacctcc gaggtcccta tttcaggacc gtggcggcgt ccacgagaac gacatcgtgc
     5941 atcaggttca gataggcttc gtagtagccc ctgtgggaag cgccgacgat cgtcagcatg
     6001 cgcgtgcccg gataccggcc cagcacgtcg cggatgttgg cgaccatgcg cagattgcgg
     6061 gtttcccagt agcccacata gcgtcgcccg aactgtccgg gcgacggttc ggcaagggcc
     6121 gcgccgaaat cggattggta gacgaccatc gccgccgcgg gatcgttgaa cgcgcgatag
     6181 aggttcagga ctccgtcggc ctcacccaga cccgcgtaga ggcgttcatc ggccgccttg
     6241 cgcgccttgc tcgcggggtt gtcccaggcg cccatgagcg cctgtccgaa ggcatcgctg
     6301 tcctcgggcg gcgtctcgct ggccgccgac tggtcgtcga cgctccacac gcgctccagc
     6361 cccagtcggg ccgcgagcgc cgcggcgacc agttcggttt cattgcgccg cgtcacccgc
     6421 cggttcaggg cctggaccag ggcctcggtc aggccatccc gggcgagccg ctcggcctcg
     6481 gggaggcgca gccactgcac gacggcggaa ccgggctcgc cggccgcgag gaggagaagc
     6541 gccatccgcc gtcgctctcc cggcgacggc gcgtctggcc aatgcgcgaa caggcgctcg
     6601 acctccgcgt tggcggccgg cacgtccagc ccggcggcca ggccggcggc cgtggggtcg
     6661 tagcagtagt ccggcactgt cgaggcgtag cgataggcgt agcgccgcag gctgtcgcac
     6721 tggagaccgg acaggttctc ggtcgagatg gcggtcggtc gccaggccgc cagccggtcc
     6781 agcaggggct cgagctgttc cggccggaag ctgtcgggca ggcctgaaag gtgcggcgtc
     6841 cccaggacca gcacctcatt gggcgctctc gtcgccggcg ccttcagctg gtcgggatga
     6901 aaggcgggtc ggtattcccg ggccgtcgcg ggcgagctga tcgccatcag cgccgccgcc
     6961 attccgatcg tcacacgcat tcgatacccg gccgctttga gcgccccgtc aacgggcggt
     7021 tcgacaagcc tgggcgtggc cacggctggc cacaagttaa accggcgtaa tcagaccccg
     7081 ggaacggccg cgcgctgagg ccgaccgtcc gaacccctgt ctccgcgccc ggcctatccc
     7141 agcgccgccc gcaccgccgc cacgccggcc gcggcctgg
//
LOCUS       DAORNX010000107         7047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7914_length_7047_cov_7.867849, whole genome shotgun sequence.
ACCESSION   DAORNX010000107 DAORNX010000000
VERSION     DAORNX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7914_length_7047_cov_7.867849"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLO65_06300"
     CDS             complement(<1..325)
                     /locus_tag="PLO65_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HRD27892.1"
                     /translation="MTDRPVPPRPGRGASRARLAEILRVDHAGELGAVHIYRGQQAVL
                     GKARGHERVAGQLAEMEGHEAVHLARFDALLNQHRVRPTLLAPLWRAAGFALGNAVGD
                     GNGTAT"
     gene            complement(318..836)
                     /locus_tag="PLO65_06305"
     CDS             complement(318..836)
                     /locus_tag="PLO65_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRD27893.1"
                     /translation="MPAALKDLIDRCLRHWPLVAALSSALMLAIAHAFETFGHLAPCT
                     LCLKAREVYWVALTVGLVAFAAQTLLKDDRARRLGSLILAAIFLFGVCLAVYHAGAEW
                     KFWPGPKTCASGALPQLTSLDAVLKGGSIKAPACDEAAWVFLGLSMAGWNALISLKLL
                     VYSLLAARLRRD"
     gene            complement(838..1434)
                     /locus_tag="PLO65_06310"
     CDS             complement(838..1434)
                     /locus_tag="PLO65_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRD27894.1"
                     /translation="MLRRLYDWVMGLAASRHAPSALFAVSFAESSFFPVPPDVMLAPM
                     VLARPEKAWRYAALCTLASVLGGCLGYAIGFYLQDAALWLMKLSGHEGGLEQFKCWYA
                     EWGVWVILIKGLTPIPYKLVTIASGLAQFAFPVFIAASVVTRGARFFLVAGVVRKFGP
                     AMLPVIERRLALFAGLAVALVVVGLVASHFLGSGTSTC"
     gene            1497..1886
                     /locus_tag="PLO65_06315"
     CDS             1497..1886
                     /locus_tag="PLO65_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD27895.1"
                     /translation="MSIRTASCACGALEARCDGEPDIVSLCSCRNCQRRTGSAFGIVA
                     VFPRAAVTVSGAAHPFSRPTDSGGETTFHFCPTCGSTVFWDRSARPEMICVAVGAFAD
                     PNFPMPVRTVYDEHRHPWIGFEAATRL"
     gene            1931..3016
                     /gene="hppD"
                     /locus_tag="PLO65_06320"
     CDS             1931..3016
                     /gene="hppD"
                     /locus_tag="PLO65_06320"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRD27896.1"
                     /translation="MNAQPKADLFDNPLGTDGFEFVEFTSPEPEKLKAIWELMGFTAV
                     GRHRSKNVLHFRQGDINFILNMEPSGQSADFRGAHGPSANAMAFRVRDAGKAYKLALE
                     RGATPAAPGKVGPMELNIPAIEGIGGTNLYLVDRYGAQSIYDVDFEPIPGAAEAAVKN
                     TVGLTYLDHLTHNVFRGNMKKWADFYERVFNFREIRYFDIEGKVTGLFSKAMTSPDGK
                     IRIPLNESQDEQSQIEEFLRDYKGEGIQHLAFGTDDIFHTVETLRERGVKFQDTPDSY
                     YELLDGRVVGHGENTQRLQADKILLDGAPTEGQGLLLQIFTENLIGPIFFEIIQRKGN
                     EGFGEGNFKALFESIELDQIRRGVVKA"
     gene            complement(3103..3318)
                     /locus_tag="PLO65_06325"
     CDS             complement(3103..3318)
                     /locus_tag="PLO65_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27897.1"
                     /translation="MSEISDIIENHLEPILASLVHIRTGLDTIKADMTSIKASVTGIE
                     FAMAGMNRSVDRGEQRLERIERRLGLP"
     gene            3342..4388
                     /locus_tag="PLO65_06330"
     CDS             3342..4388
                     /locus_tag="PLO65_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRD27898.1"
                     /translation="MSLQQRLLALVVTLALGVSAGPALARPWAIVIHGGAGVIERKDL
                     TPETEAAYRAAMARAVATGSAVLEKGGSSMDAVEAVIREFEDDPLFNAGRGAVFTAEG
                     TNSLDSAIMDGATLRAGAVAGVTRTRHPISLARAVMEKSKHVMLVGPGADAFSVSAGL
                     EQAEPAWFFTERRWAQLVKALPADGLPVPPRPQGAPAPHAFIAIPDDRKFGTVGVVAL
                     DAAGNVAAGTSTGGTTAKRWGRVGDSPIIGAGTYASNQSCAVSATGTGEYFIRLTVAR
                     EICALVQYQGMSLQKAADTVIQDRLTALGGDGGVIALAPDGQIAWSYNTSGMYRARAA
                     QGQPSVISIYKDEP"
     gene            4391..5524
                     /locus_tag="PLO65_06335"
     CDS             4391..5524
                     /locus_tag="PLO65_06335"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRD27899.1"
                     /translation="MANRNWIPVRGAEGLHSKQAHADLPEGTYEREMSKEGFFGPAAF
                     LYHRRPPTGWTSFEGPLRPRAYDLNGLNEAAPSPWDAAVVLHNAACEMRFWKLSAAMP
                     GLARNADGDQLLFVHAGSGELFCDFGRLAYEAGDYLYLPRGTMWRLSPAAPTAILTIQ
                     ATNGHFTLPDKGLLGPHALFDPALLVTPVMDEAFRAHQAEEGTYPVAIKKRGQVSVAT
                     YPWNPLDAVGWHGELAPVKINVRDFRPLNSHRYHLPPSVHTTFLADRFVVCTFAPRPF
                     ETDPGAIKIPFFHNNDDYDEVLFYHAGDFFSRDGIDAGMMTFHPSGFTHGPHPKALKN
                     MLVQTKPATDEYAVMIDTRDPLEVGEGAAAVENAAYVDSWKGA"
     gene            5521..5796
                     /locus_tag="PLO65_06340"
     CDS             5521..5796
                     /locus_tag="PLO65_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27900.1"
                     /translation="MSDAADPHLLAMAEDELGRALTIRWRDLAKITPWGDTFEGISPD
                     GRNVEVERNYLWASEPGGDILVEIAVFGGPSRYDAGARVSRLISKTA"
     gene            5831..6595
                     /locus_tag="PLO65_06345"
     CDS             5831..6595
                     /locus_tag="PLO65_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27901.1"
                     /translation="MTDHSEFPWGPQYEPGLLTPFGVIGWVVATALLLLLVLRMREGA
                     GGKAGDAHLQEMHKVILQAARYALKQDEYGILQGAKVLRRTISFYLGGLLMASEPIGK
                     QAKALALAMGEKDEKKDDKAKGGHGKDAHGKDAHGKAGHAAHAGHGGHAPAAEHAHAG
                     AAPGVAAHTVQGHSITVNVGGEGGGHHDEAHEEEEEKPLSLEEQLIACRKAVREFEAW
                     WSDKQARIAQLKAAQDALTIPRKPDPALIALFEEKK"
     gene            6592..>7047
                     /locus_tag="PLO65_06350"
     CDS             6592..>7047
                     /locus_tag="PLO65_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD27902.1"
                     /translation="MKLASLKGGRDGRLVVVSHDLAWCTSAEAIAPTLQAALDDWDRC
                     EPLLRGLAESLEHGALPRDRFHEHDAASPLPRAYQWADGSAYVNHVALVRQARAAEMP
                     ASFWTDPLMYQGGSDGFLGPRDPIPLKDEAWGCDLEGEVAVVTGDVPLGA"
BASE COUNT         1192 a         2432 c         2424 g          999 t
ORIGIN      
        1 aggtcgcggt gccgttgccg tcacccaccg cattgcccag cgcgaagccg gcggcgcgcc
       61 acaagggcgc cagcagggtc ggccgcacgc ggtgctggtt gagcagggcg tcgaaccgcg
      121 ccaggtgcac ggcctcgtgg ccttccatct cggccagctg tccggccacc cgctcatggc
      181 cgcgcgcctt gcccagcacg gcctgctggc cgcgatagat gtgcaccgcg ccgagttcgc
      241 cggcgtggtc gacgcgcagg atctcggcca ggcgggcccg gcttgcgccg cgaccgggac
      301 ggggcgggac agggcgatca gtcacggcga agcctcgcgg ccagaaggct gtagaccaga
      361 agcttgagcg aaatcagcgc gttccagccg gccatggaca ggccaaggaa cacccaggcg
      421 gcctcgtcgc aggccggcgc cttgatgctg ccgcccttca gcacggcgtc caggctggtc
      481 agctgcggca gggcgccgga cgcgcaggtc ttgggaccgg gccagaactt ccattcggcc
      541 ccggcgtgat agacggccag gcagacgccg aacaggaaga tggccgccag gatcaggctg
      601 cccagccggc gggcccggtc gtccttgagc agggtctggg ctgcgaaggc gaccaagccc
      661 accgtcaggg ccacccagta tacctcccgc gccttcaggc agagggtgca gggcgccagg
      721 tgaccgaagg tctcgaaggc atgggcgatg gccagcatca gcgccgacga cagggccgcg
      781 accagcggcc agtggcgcag gcagcggtcg atcaggtctt tcagcgcggc gggcatgtca
      841 gcaggtgctc gttccggacc cgaggaagtg gctggcgacc aatcctacca cgaccagggc
      901 gaccgcaagc ccggcgaaca gcgccagccg ccgctcgatc accggcagca tggccgggcc
      961 gaacttcctg accaccccgg cgacgaggaa gaaccgcgcg ccgcgggtca ccaccgaggc
     1021 ggcgatgaac acggggaagg cgaactgggc caggcccgag gcgatggtca ccagcttgta
     1081 ggggatcggc gtcaggccct tgatcaggat cacccagacg ccccactcgg cgtaccagca
     1141 cttgaactgc tccaggccgc cctcgtggcc cgacagcttc atcagccaaa gggcggcgtc
     1201 ctggaggtag aagccgatcg catagccgag gcagccgccg agcaccgagg ccagggtgca
     1261 cagggccgcg tagcgccagg ccttctctgg ccgggccagc accatcggcg ccagcatcac
     1321 gtccggcggc accggaaaga aggagctctc ggcgaacgag accgcgaaca gggcggaggg
     1381 ggcgtggcgc gaggcggcga gccccatcac ccagtcgtaa agtcggcgca gcatgcggct
     1441 gtgctatcag ggccgcgcgg ccttgtctcg cgcctgcacg gaaaagggac gccccgatgt
     1501 cgatacgcac cgcttcctgc gcctgcgggg ccctcgaggc ccgctgcgac ggcgagcccg
     1561 acatcgtctc cctgtgcagt tgccgcaact gccagaggcg cacgggctcg gccttcggca
     1621 tcgtggcggt cttcccgcgc gcggccgtga ccgtcagcgg ggcggcgcac ccgttctcgc
     1681 gcccgacgga cagcgggggc gaaaccacct tccacttctg cccgacctgc ggctcgacgg
     1741 tgttctggga ccgctcggcc cggccggaga tgatctgcgt cgccgtcggc gccttcgccg
     1801 acccgaactt cccgatgccg gttcgcaccg tctacgacga gcatcgccat ccgtggatcg
     1861 gattcgaggc ggccactcga ctctagtttc atttgttact atctctgccg accttcgaag
     1921 gagactcgcg atgaacgccc aacccaaggc cgacctgttc gacaacccgc tcggcaccga
     1981 cggcttcgag ttcgtcgagt tcacctcgcc cgagcccgag aagctcaagg cgatctggga
     2041 gctgatgggc ttcaccgcgg tcggcaggca ccgctccaag aacgtgctgc atttccgtca
     2101 gggcgacatc aacttcattc tcaacatgga gccgtccggc cagtcggccg acttccgcgg
     2161 cgcgcatggc ccgtcggcca acgccatggc cttccgcgtg cgtgacgccg gcaaggccta
     2221 taagctggcg ctcgaacgcg gcgccacgcc cgcggcgccc ggcaaggtcg ggccgatgga
     2281 gctgaacatc ccggccatcg agggcatcgg cgggaccaac ctgtatctgg tcgaccgcta
     2341 cggcgcgcag agcatctacg acgtcgactt cgaaccgatc ccgggcgcgg ccgaggcggc
     2401 ggttaagaac accgtcggcc tgacctacct ggaccacctg acccacaacg tgttccgcgg
     2461 caacatgaag aagtgggcgg acttctacga acgggtcttc aatttccgcg agatccgcta
     2521 cttcgacatc gagggcaagg tcacgggcct gttctccaag gcgatgacca gcccggacgg
     2581 caagatccgc attccgctca acgagagcca ggacgagcag agccagatcg aggagttcct
     2641 gcgcgactac aagggcgaag gcatccagca cctggccttc ggcacggatg acatcttcca
     2701 caccgtcgaa acgctgcgcg agcggggcgt gaagttccag gacacgcccg acagctacta
     2761 cgagctgctg gacggccggg tcgtcggcca tggcgagaac acccagcggc tgcaggccga
     2821 caagatcctg ctcgacggcg ctccgaccga gggccagggc ctgctgctgc agattttcac
     2881 ggaaaacctg atcggcccga tcttcttcga gatcatccag cggaagggca acgagggctt
     2941 cggcgaaggc aacttcaagg ccctgttcga gtcgatcgaa ctcgaccaga tccgccgcgg
     3001 cgtggtgaag gcgtagatct cgctcctctc ccgcgaggga gagggggacc atgcggagca
     3061 tggtggagag ggaagcgccg cggtgaaggg ctattcgagg tgtcagggca atcccaggcg
     3121 ccgttcgatt cgttcgaggc gctgctcccc gcggtccacg gaccggttca tgccggccat
     3181 ggcgaattcg atgccggtga cgctcgcttt gatgctcgtc atgtcggcct tgatcgtgtc
     3241 gagaccggtg cgaatgtgga ccaacgacgc gaggatcggt tcgaggtgat tttcgatgat
     3301 gtcgctgatc tcggacatgc cgtgagaatg gagccgaaaa gttgagcctg caacaaagac
     3361 ttctcgccct ggttgtcacg ttggcgctcg gcgtctcggc tggccccgcc ctcgccaggc
     3421 cctgggcgat cgtcatccac ggcggggcgg gggtcatcga gcgcaaggac ctgacgccgg
     3481 agaccgaggc cgcctatcgc gcggccatgg cccgggcggt cgccaccggt tcggctgtgc
     3541 tcgagaaggg cggctcgtcg atggacgcgg tcgaggcggt gatccgcgag ttcgaggacg
     3601 atccgctgtt caatgccggc cgcggcgcgg tgttcaccgc cgagggaacc aactcgctcg
     3661 attcggcgat catggacggc gcgacgctca gggccggcgc cgtcgccggc gtcacccgca
     3721 cccgccatcc gatcagcctg gcccgggcgg tgatggagaa gtcgaaacac gtgatgctgg
     3781 tcggacccgg cgccgacgcc ttctcggtct ccgctgggct ggagcaggcg gagccggcgt
     3841 ggttcttcac cgagcggcgc tgggcgcagc tggtcaaggc gctgcccgcc gacggcctgc
     3901 cggtcccacc ccggccgcag ggggcgccgg cgccgcacgc cttcatcgcc atcccggacg
     3961 accgcaagtt cggcacggtc ggggtcgtgg ccctcgacgc cgccggcaat gtcgccgccg
     4021 gcacctcgac gggcggcacc acggccaagc gctggggccg ggtgggcgac agcccgatca
     4081 tcggcgcggg cacctacgcc tccaaccagt cctgcgccgt ctccgccacg ggcacgggtg
     4141 agtatttcat ccgcctgacg gtggcccggg agatctgcgc cctggtgcaa taccagggca
     4201 tgagcctgca gaaggccgcc gacaccgtga tccaggaccg gctgacggcg ctgggcggcg
     4261 acggcggcgt gatcgccctg gcccccgacg gccagatcgc ctggagctac aacaccagcg
     4321 gcatgtaccg cgcccgcgcc gcccaggggc agccgagcgt gatttcgatc tacaaggacg
     4381 agccctgacc atggccaatc gaaactggat tcccgtccgc ggcgccgaag gccttcacag
     4441 taagcaggcc catgcggacc tgcccgaggg cacctacgag cgggagatgt ccaaggaggg
     4501 cttcttcggc ccggcggcct tcctctatca ccgcaggccg ccgaccggct ggaccagctt
     4561 cgaggggccg ctgcggccgc gcgcctatga cctgaacggc ctgaacgagg ccgcgccttc
     4621 gccgtgggac gcggccgtcg tgctgcacaa cgccgcctgc gagatgcgct tctggaagct
     4681 gtcggccgcg atgcccggtc tcgcccgcaa cgccgacggc gaccagctgc tgttcgtcca
     4741 cgccgggtcg ggcgagctgt tctgcgactt cggccgtctc gcctatgagg ccggcgacta
     4801 cctttacctg ccgcgcggga ccatgtggcg gctcagcccc gcggcgccga ccgcgatcct
     4861 gacgatccag gcgaccaacg gccatttcac cctgccggac aagggcctgc tgggcccgca
     4921 cgcgctgttc gacccggccc tgctggtcac cccggtcatg gatgaggcct tccgcgccca
     4981 ccaggcggag gaggggacct atcccgtggc gatcaagaag cgcggccagg tctcggtggc
     5041 gacctatccg tggaatccgc tcgacgcggt cggctggcac ggcgaactgg ccccggtgaa
     5101 gatcaacgtg cgcgacttcc gcccgctgaa cagccaccgc taccacctgc cgccgagcgt
     5161 ccacacgacc ttcctggccg accggttcgt ggtctgcacc ttcgccccgc gaccgttcga
     5221 gaccgacccg ggggcgatca agatcccctt cttccacaac aacgacgact acgacgaggt
     5281 gctgttctac cacgcgggcg acttcttcag ccgcgacggc atcgacgccg ggatgatgac
     5341 cttccacccg tccggcttca cccacggccc gcaccccaag gcgctgaaga acatgctggt
     5401 gcagaccaag ccggcgaccg acgaatacgc cgtgatgatc gacacccgcg atccgctcga
     5461 ggtcggcgag ggcgcggcgg cggtcgagaa cgccgcctat gtcgacagct ggaagggcgc
     5521 atgagcgacg ccgccgatcc ccaccttctc gccatggccg aggacgaatt gggacgcgcc
     5581 ctgaccatcc gctggcgcga tctggccaaa atcaccccat ggggcgacac cttcgaggga
     5641 atttcccctg acgggagaaa tgtcgaggtc gaacgaaact atctgtgggc gagcgagccc
     5701 ggaggggaca ttctggtcga gattgcggtg ttcggcgggc cgtcgcgcta cgatgcgggg
     5761 gccagggtca gccgcctgat ttcgaagacc gcctgaacgg cggcttggga gtcgtcgtag
     5821 gggtgcgaag atgacggatc acagtgaatt tccctggggg ccccagtacg agccgggcct
     5881 gctgacaccg ttcggcgtga tcggctgggt cgtggccacg gcgctgttgc tgctgctggt
     5941 cctgcggatg cgcgagggcg cgggcggcaa ggccggcgac gcccatctgc aggagatgca
     6001 caaggtcatc ctgcaggcgg cccgctacgc cctgaagcag gacgagtacg gcatcctgca
     6061 gggcgccaag gtgctgcgcc ggacgatcag cttctatctc ggcggcctgc tgatggccag
     6121 cgagccgatc ggcaagcagg ccaaggccct ggcgctggcg atgggcgaga aggacgagaa
     6181 gaaggacgac aaggccaagg gcggccacgg caaggacgct cacggcaagg acgctcacgg
     6241 caaggcgggc cacgcggccc acgccggcca tggcgggcat gccccggcgg ccgagcacgc
     6301 ccacgccggc gcggcgccgg gcgtggcggc ccacaccgtt cagggccaca gcatcaccgt
     6361 caacgtcggc ggcgagggcg gcggccatca cgacgaggcg cacgaggagg aggaggaaaa
     6421 gcctctcagc ctcgaggagc agctgatcgc ctgccgcaag gcggtgcgcg agttcgaggc
     6481 ctggtggagc gacaagcagg cccgcatcgc ccagctcaag gcggcccagg acgcgctgac
     6541 cattccccgc aagccggacc cggctctcat cgccctcttt gaagagaaaa aatgaaactt
     6601 gcgtccctca agggcggccg ggatggccgt ctcgtcgtcg tttcccacga tctggcctgg
     6661 tgtacgtcgg cggaggccat cgctccgacc ctgcaggcgg cgctcgacga ctgggaccgc
     6721 tgcgagccgc tgttgcgcgg cctggccgag agtctcgagc acggcgccct gcccagggac
     6781 cggttccacg agcacgacgc cgcctcgccg ctgccgcgcg cctaccagtg ggccgacggc
     6841 agcgcctatg tgaaccacgt ggccctggtg cggcaggccc gcgcggcgga gatgccggcc
     6901 agtttctgga ccgatccgct gatgtaccag ggcggctcgg acggcttcct cggcccgcgc
     6961 gacccgattc cgctgaagga cgaggcctgg ggctgcgacc tcgagggcga ggtggcggtg
     7021 gtcaccggcg acgtgccgct cggcgcc
//
LOCUS       DAORNX010000108         7046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7916_length_7046_cov_6.535260, whole genome shotgun sequence.
ACCESSION   DAORNX010000108 DAORNX010000000
VERSION     DAORNX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7916_length_7046_cov_6.535260"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLO65_06355"
     CDS             complement(<1..299)
                     /locus_tag="PLO65_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRD27903.1"
                     /translation="MRAFARLLDRLAFTASRNAKLRLVRDFLRDTPDPDRGWALAALT
                     GSLSFSAAKPAAIRKAVEARMDPVLFGWSYDYVGDLAETVALVWPARPGANREPEL"
     gene            complement(363..1085)
                     /locus_tag="PLO65_06360"
     CDS             complement(363..1085)
                     /locus_tag="PLO65_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27904.1"
                     /translation="MKTVRRDYRTLSSAEIVTTVGRLADRIGERFPDTGLAAAARQLN
                     AIARDTARRARGLSRPYLILRTLVLLVTASGVAVFARILKDVDWAGIAARRNDLALSQ
                     TLESSVNLIVLMGAGLWFLLTLEERWKRARTQTALHELRAFAHVVDMHQLTKDPTVLL
                     GGGGRTPSSPERRMTRFELTRYLEYCAEMLALTGKLSALYAGESHDHVVIAAASDVET
                     LCTDLGRKIWQKITILGDLAEN"
     gene            complement(1260..2438)
                     /locus_tag="PLO65_06365"
     CDS             complement(1260..2438)
                     /locus_tag="PLO65_06365"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD27905.1"
                     /translation="MKALFFASTALVLASAPVAAHAQDWKVDGSLGASLGTTSNVLAA
                     PSNETSDTVYGGSGKLTLSTDSDAGSFSMFGSFDLTHYADAEDENADDFAIGADGTVK
                     FTGGSLFAGASHALTTEDRRAVTARRDTRSPTESTVDSFDAGFTTRLGGVGFSARADY
                     VTSDFEDARHRITNAVIDQDFRDRGTWTETFRVTGSPDADVSLFAEVAFTQVDYDQQP
                     PASLHNRDSDGYTASVGVTFDLTGQLSGEVSIGWSGRSFDEPAFDDATDFVVDADLTW
                     TPTTDTSIELTAARSFQESTTPLSPIYVATSVGASISHNYTPDFRVAAYVGQEWDDHE
                     NRDRQDSTGSAGVSAAYSVAANAELSVAYDFSTQDSDGVHAIPGYDDGRWTVGFKIGF
                     "
     gene            complement(2714..3721)
                     /locus_tag="PLO65_06370"
     CDS             complement(2714..3721)
                     /locus_tag="PLO65_06370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HRD27906.1"
                     /translation="MLKPQALLHPTPAGLYCPPGDFHIDPVRPVARAVITHGHADHAR
                     AGHAQVAATRETLDIMAARYGEAFAGATRALAYGETIARDGVEITLVPAGHVLGSAQV
                     VVRWKGMTVVVSGDYKRRRDPTCPPFEPVPCDVFVTEATFGLPVFRHPDAADEVTRLL
                     RSVEQFPERTHLVGAYALGKAQRIVALLREAGHDRTIHVHGAMEALNGLYGRHGVELG
                     PLAPATGADGDLAGEIVICPPSALGDRWSRRFADPVSAFCSGWMRVRARARQRGVELP
                     LIVSDHADWDELTGTLAELAPGEVWITHGREEALLRWCELRGQPARALALVGYDEEDG
                     D"
     gene            3758..4234
                     /locus_tag="PLO65_06375"
     CDS             3758..4234
                     /locus_tag="PLO65_06375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD27907.1"
                     /translation="MSDHLDAMRNFVQAMNQGSPHAIALKMETVSLDADGAVLRVPYD
                     EKLIGDPDTGVIAGGVVTALLDHACGQAVHTGLTEFKTIATLDLRIDYMRPAEPGKDI
                     FAKASCYKVTRSVAFVRAVAYDVDPADPVAAAQAAFMLDSSGGRGFGANLKDRKPR"
     gene            4231..4653
                     /locus_tag="PLO65_06380"
     CDS             4231..4653
                     /locus_tag="PLO65_06380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD27908.1"
                     /translation="MSDPAESIDAMLARIPYARFLGLRIELAGDEMTAILPFGQHLIG
                     NPTLPAIHGGVLGAFMEMTALAQLLVKEGQARQPRVIDVSIEYLRSGRPLTTYARAEI
                     KKVGRRIANVHVEAWQDQRAAPIAALRGHFLVTPTERA"
     gene            5183..5860
                     /locus_tag="PLO65_06385"
     CDS             5183..5860
                     /locus_tag="PLO65_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD27909.1"
                     /translation="MQAPAAVIFDCDGVLVDSEILAIEVEITLLAECGLVYHPDDYRH
                     RFLGLNDAAFREALTRDCLDRTGRPLPDDFLRRAHDQRWDACQTRLTEVAGCGAAIAA
                     LNRPRAVASSSGAAFLREKLRLTGLLEAFDPHVYSADLVARAKPHPDIFLHAASALAT
                     DPARCLAIEDSVNGVRSALAAGMTVWGFAGGGHMDDGSSARLMEAGATRLVADWREAR
                     GLFAAFG"
     gene            complement(5909..6568)
                     /locus_tag="PLO65_06390"
     CDS             complement(5909..6568)
                     /locus_tag="PLO65_06390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD27910.1"
                     /translation="MQQIPDLPRPPLDDETIAGRTVDLERANLLRHGEDLWRAIGSRD
                     ELWGGIPSGPFESEAVFYDWLGSRLGREDQRAYAIIDRSDGGRAAAGLFFLLQVKPDM
                     GTCELGLVYGPSLSRTVAGTEAVYRLARYVMETNGYRRLEWRCGPDNLASVRAASRYG
                     FTYEGMLRQTYWLKGHNWDTRIYSILDSEWPAIGARFEAWLAPENFDAAGRQIKGLGE
                     I"
     gene            6677..>7046
                     /locus_tag="PLO65_06395"
     CDS             6677..>7046
                     /locus_tag="PLO65_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRD27911.1"
                     /translation="MLKSLAGRVLIALVLGLGAGAACHAIGDPTLDGLAGAVEAVGGL
                     WLNLLRMTIVPLVFCLLVTGIVSVADAAATGRLAARAILLFSVGMLVAAVYGTLAPQG
                     VLALWPVSPDGAAALRAGAGG"
BASE COUNT         1043 a         2485 c         2474 g         1044 t
ORIGIN      
        1 agctccggct cgcggttggc gccgggccgc gcgggccaga ccagggcgac cgtctcggcg
       61 aggtcgccga catagtcgta ggaccagccg aacaaaaccg ggtccatgcg ggcctcgacc
      121 gccttgcgga tggcggccgg cttggcggcg gagaagctca gcgagcccgt cagggcggcc
      181 agcgcccagc cgcggtccgg gtcgggcgtg tcgcgcagga agtcgcgcac cagccgcagc
      241 ttcgcgttgc gcgaggcggt gaaggcgagg cggtcgagca ggcgggcgaa ggcgcgcatg
      301 ggaagggaat gtagatcaag accaaggcgt ttcctccctc cccatgaagg tgacgcagag
      361 gctcaattct ccgccaggtc gccgagaatg gtgatcttct gccagatctt gcggccaaga
      421 tcggtgcaca gggtttcgac gtcgctggcg gcggcgatca ccacatggtc atgactctcg
      481 ccggcgtaga gggccgacag cttgccggtc agggccagca tctcggcgca gtattcgaga
      541 tagcgcgtca gctcgaaacg cgtcatgcgc cgctccggcg aggacggcgt ccggccgccg
      601 ccgccgagca gcaccgtcgg gtccttggtc agctggtgca tgtcgaccac atgggcgaac
      661 gcccgcagct cgtgcagcgc ggtctgggtg cgcgcccgtt tccagcgctc ctcgagggtc
      721 agcaggaacc acaggcccgc gcccatcagg acgatcaggt tgacgctcga ttcgagcgtc
      781 tgcgacaggg ccagatcatt gcgccgcgcg gcgatgccgg cccagtcgac gtccttgagg
      841 atccgggcga acacggccac cccgctggcc gtcaccagca gcaccagggt gcgcaggatc
      901 agataggggc gcgacaggcc tcgcgcccgc cgcgccgtgt cccgggcgat ggcgttgagc
      961 tgcctcgccg ccgcggccag gccggtgtca gggaagcgtt cgccgatgcg gtcggcgagc
     1021 cggccaacgg tcgtgacgat ctcggccgag ctgagcgtcc ggtagtcgcg ccgcaccgtc
     1081 ttcatgagac cgccccgtcc cgccgccaac cccggcggcc gggcgggagc agaccatgcg
     1141 gcgacggccc gcgccacccc cagcgcccga cggtggaaga tccgtcgccg gacacgggaa
     1201 gcggggcgcc gcgggccgcg tccggcgacg tcttccggaa tcgacgcggg gcgacgcccc
     1261 tagaagccga tcttgaagcc gaccgtccag cgtccgtcgt catagccggg gatggcgtgc
     1321 acgccgtccg agtcctgggt cgagaagtcg taggccacgc tgagctcggc gttcgccgcg
     1381 acgctgtagg cggccgagac gccggccgag ccggtcgaat cctggcggtc gcggttctcg
     1441 tgatcgtccc attcctgccc gacataggcc gccacgcgga agtcgggcgt gtagttgtgc
     1501 gagatgctcg cgccgaccga cgtggcgacg tagatcggcg acagcggcgt ggtgctttcc
     1561 tggaagctgc gggcggcggt cagttcgatc gaggtgtcag tcgtcggcgt ccaggtcagg
     1621 tcggcgtcga ccacgaagtc ggtcgcatcg tcgaaggccg gctcgtcgaa cgaccggccg
     1681 ctccagccga tgctgacctc gccgctcagc tggccggtga ggtcgaacgt caccccgacg
     1741 ctggcggtat agccgtcgga atcgcggttg tgcagcgagg ccggcggctg ctggtcatag
     1801 tcgacctggg tgaaggccac ctcggcgaac agcgacacat cggcgtccgg cgagccggtg
     1861 acccggaagg tctccgtcca ggttccccgg tcgcggaagt cctggtcgat gaccgcgttg
     1921 gtgatgcggt gacgggcgtc ctcgaagtcg gaggtgacat agtcggcgcg ggccgagaag
     1981 ccgacgccgc ccaggcgcgt ggtgaagccg gcgtcgaagc tgtcgacggt gctttcggtc
     2041 gggctgcgcg tgtcgcgacg ggcggtgacc gcccggcggt cctcggtggt cagggcgtgg
     2101 ctggcgccgg cgaacaggct gccgccggtg aatttcacgg tcccgtccgc gccgatcgca
     2161 aagtcatcgg cgttttcgtc ttcggcgtcg gcgtagtggg tcaggtcgaa gctgccgaac
     2221 atcgagaagc taccggcgtc gctatcggtg ctcagggtca gcttgcccga gccgccgtag
     2281 acggtatcgc tggtctcgtt cgagggcgcg gccagcacgt tgctggtcgt gcccagcgag
     2341 gcgccgagcg aaccgtcgac cttccagtcc tgggcatgcg ccgccaccgg cgccgacgcc
     2401 agaacaagcg ccgtcgaagc aaaaaacaag gccttcatgg tattccccaa cttgtcttgg
     2461 gcgccgctcg cgacgccacg ccttcgcggc gcctggacag gcgcgcgcag cccggaaaac
     2521 cggcgcagcc gcgcgaaagc cgagacatcc gccactcaga cgcgccggtc tcgccggctg
     2581 cgccacccca acgcggtcga cgctattcaa cattccggcg gcgcgaacag gacgcgccta
     2641 cccgatctgt ggaaacggcg gaaacccgga tatcgagacg cgcgagggcg acattcggga
     2701 cacagtggcc ggatcagtcc ccgtcttcct cgtcgtagcc gaccagggcc agggcccgcg
     2761 ccggctggcc gcgcagctcg caccagcgca gcagggcctc ctcgcggcca tgggtgatcc
     2821 acacctcccc gggggccagt tcggcgaggg ttccggtcag ttcgtcccag tcggcgtggt
     2881 cggaaacgat cagcggcagc tcgacgccgc gctgcctggc gcgggcccgc acgcgcatcc
     2941 agccggaaca gaaggcgctg accgggtcgg cgaaccgccg gctccagcgg tcgccgaggg
     3001 ccgacggcgg gcagatcacg atctctccgg cgaggtcgcc atcggccccc gtggccggcg
     3061 ccagcggtcc cagctcgacg ccgtgccggc catagaggcc gttgagcgcc tccatcgccc
     3121 cgtggacgtg gatggtccgg tcatgtccgg cctcgcgcag cagggcgacg atccgctggg
     3181 ccttgcccag cgcgtaggcc ccgaccagat gcgtgcgttc agggaactgc tcgaccgacc
     3241 ggagcagccg cgtcacctcg tcggcggcgt ccggatgacg gaagacgggc aggccgaagg
     3301 tcgcctcggt cacgaacacg tcgcagggca cgggctcgaa gggcgggcag gtcggatcgc
     3361 gccgccgctt gtagtcgccc gagacgacca ccgtcatgcc cttccagcgc acgaccacct
     3421 gggccgagcc cagcacgtgg ccggccggga ccagggtgat ctcgacgccg tcgcgcgcga
     3481 tggtctcgcc ataggccagc gcgcgggtgg cgcccgcgaa ggcctcgccg tagcgcgcgg
     3541 ccatgatgtc cagggtctcg cgtgtggccg ccacctgcgc gtggccggcg cgggcgtggt
     3601 cggcgtggcc atgggtgatc acggcccgcg ccacggggcg cacggggtcg atgtggaagt
     3661 cgcccggcgg acaatagagg cccgccgggg tcgggtgcag cagggcctgc ggtttcagca
     3721 tctggataag ccaacgcgcg acgtctaaac ccgagccatg agtgatcatc tcgacgccat
     3781 gcgcaacttc gtccaggcca tgaaccaggg cagcccgcac gccatcgccc tgaagatgga
     3841 gaccgtctcg ctcgacgccg acggggcggt cctgcgggtg ccctatgacg agaagctgat
     3901 cggcgatccc gacacgggcg tgatcgccgg cggtgtcgtc acggccctgc tcgaccacgc
     3961 ctgcggccag gccgtgcaca cgggcctgac cgagttcaag accatcgcca ccctcgacct
     4021 gcgcatcgac tacatgcggc ctgccgagcc ggggaaggac atcttcgcga aggccagctg
     4081 ctacaaggtg acccgctcgg tcgccttcgt gcgggccgtg gcctacgatg ttgatccggc
     4141 cgacccggtg gccgcggccc aggcggcctt catgctcgat tccagcggcg gccgcggctt
     4201 cggcgccaat ctgaaggacc gcaagccccg atgagcgatc cggccgaatc catcgacgcc
     4261 atgctggcgc gtattccgta cgcccggttc ctcggcctgc gcatcgagct ggccggcgac
     4321 gagatgaccg ccatcctgcc gttcggccag cacctgatcg gcaacccgac cctgccggcg
     4381 atccacggcg gggtgctggg cgccttcatg gagatgacgg cgctggccca gctgctggtc
     4441 aaggaaggcc aggcgcgcca gccgcgggtc atcgacgtgt cgatcgaata cctgcgctcg
     4501 ggccgccccc tgacgaccta cgcccgcgcc gagatcaaga aggtcggccg gcgcatcgcc
     4561 aacgtccatg tcgaggcctg gcaggaccag cgcgccgccc cgatcgccgc cctgcgcggc
     4621 cacttcctcg tgacgccgac ggagagggcg taggggttag ggagtaggga gtagggagta
     4681 gggggcaggg gctgacaggc cgccgcaacc gcgtgacacc gctcagctac ttcctactcc
     4741 ctaatccctg gccccgactt cccccgcccc atcattaccg ccatgtaatc accttacatt
     4801 cgcttcacat gcttaaatcg aagtcatgac tcatacttaa cgggtgttcc gccgaacact
     4861 tgcctatacc cctgatcacc ggacgcggac ttccgcggcc acacagatcc aaggggaatt
     4921 caacatgtcg accaagtccg ttcatcagtt cagcaacctc gccagcctgg ccctcgccgt
     4981 cctgccgctg gtcatcatcc tcgccgccgt gaacttcggc gtcgtcggtg tcgcgggcct
     5041 gtgatccgcg ccgcgccgct ccgccggacc tgagtcctcc ccccgggtcg ggtccggcgg
     5101 agcaaggcgg ccttacaggc cgcaaccaga gttgactgac aaaggccttc cgcatcggga
     5161 ggcccttttg ttatgcgcgg gcatgcaagc gcccgccgcc gtgatcttcg actgcgacgg
     5221 agtcctggtg gactccgaga tcctcgccat cgaggtggag atcaccctgc tggccgagtg
     5281 cggcctggtc taccatcccg acgactaccg ccaccggttc ctgggcctta acgacgcggc
     5341 gttccgcgag gccctgacgc gggactgcct ggatcgcacg ggccggccgc tgccggacga
     5401 tttcctgcgt cgcgcccacg accagcgctg ggacgcctgc cagacccgcc tgaccgaggt
     5461 cgccggctgc ggcgcggcca tcgcggcgct gaaccggccc agggccgtgg ccagctcctc
     5521 cggcgcggcc ttcctgcgcg agaagctgcg cctgaccggc ctgctcgagg ccttcgaccc
     5581 gcatgtctac tcggccgacc tcgtggccag ggccaagccg caccccgaca tcttcctgca
     5641 cgcggccagc gcgctggcga ccgacccggc gcgctgcctg gccatcgagg acagcgtcaa
     5701 cggcgtgcgc tcggcccttg ccgccggcat gaccgtctgg ggcttcgccg gcggcggtca
     5761 catggacgac ggatcgtcgg cccgcctgat ggaggcgggg gcgacgcgcc tggtggcgga
     5821 ctggcgggag gccagggggc tgttcgcggc gttcggctaa gcgtcgttgt catcccggat
     5881 cgccgccgag cagcttccgg gacggcagtc agatctcgcc cagccctttg atctgccgcc
     5941 ccgccgcgtc gaaattctcc ggcgccagcc aggcctcgaa ccgggcgccg atggccggcc
     6001 attcgctgtc gaggatcgaa tagatgcggg tgtcccagtt gtggcccttg agccagtagg
     6061 tctggcgcag catgccctcg taggtgaagc cgtagcgcga ggccgcccgc acggaggcga
     6121 ggttgtcggg gccgcagcgc cattccagcc ggcggtagcc gttggtctcc atcacgtagc
     6181 gggccaggcg atagaccgcc tcggtgccgg cgacggtgcg gctcagcgac gggccataga
     6241 ccaggcccag ctcgcaggtc cccatgtccg gcttgacctg cagcaggaag aacaggcccg
     6301 ccgccgcgcg accgccatcg ctcctgtcga tgatcgcgta ggcgcgctgg tcctcgcgcc
     6361 ccagccggct gcccagccag tcgtagaaga ccgcttcgga ctcgaacggg ccggacggaa
     6421 tgccgcccca gagctcgtcc cgcgagccga tcgcgcgcca caggtcttcg ccgtgccgca
     6481 gcaggttggc ccgttccagg tcgacggtgc ggccggcgat ggtctcgtcg tcgagcggcg
     6541 ggcggggaag atcgggaatc tgttgcatcg ggcgagccta gagccgccgc gccctcgtcg
     6601 gcaatcgctg cggcgacacc cttccaaatc cccggccggc ggctaggatc ggccgggcgc
     6661 tagccggagt cgcccgatgt tgaagtccct cgccggacgc gtgctgatcg ccctggtcct
     6721 tggcctcggc gcgggcgccg cctgccacgc catcggcgat ccgaccctgg acggcctggc
     6781 cggcgcggtc gaggcggtcg gaggcctgtg gctgaacctg ttgcgcatga ccatcgtgcc
     6841 gctggtcttc tgcctgctgg tcaccggcat cgtctcggtg gccgacgcag cggcgaccgg
     6901 ccggctggcg gcgcgggcca tcctgctgtt ctcggtcggc atgctggtcg ccgcggtcta
     6961 tggcaccctg gcgccccagg gcgtcctggc gctctggccg gtcagcccgg acggcgcggc
     7021 cgccctgcgc gccggcgcgg gcgggg
//
LOCUS       DAORNX010000109         7042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7926_length_7042_cov_6.695864, whole genome shotgun sequence.
ACCESSION   DAORNX010000109 DAORNX010000000
VERSION     DAORNX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7926_length_7042_cov_6.695864"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /gene="ggt"
                     /locus_tag="PLO65_06400"
     CDS             <1..1597
                     /gene="ggt"
                     /locus_tag="PLO65_06400"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD27912.1"
                     /translation="GAAATAHPLATLAAIDILRRGGSAVDAAIAANACLGLLEPISSG
                     IGGDCFVMLWDPKTKKVVGLNGSGRSPRGLTLETQRARSKNGHIASFGAISVSVPGAV
                     QAWWDLHGKFGKLPWKDLFGPAIGYADEGTPVTQNVAYYLAASFRRFNNPASNIEEVV
                     NFNKVWAAEGRTPREGELFRNPALASTYRAIAAGGRDAFYDGEIADRIEAYFKRIGGW
                     MTRADLAAHHSEWTTPLVTGYRGVDVYGLAPNSQGLSTLQLLNILETFDVKAMGFQSA
                     QAIHHAVEAKRLAYEDRARYFADPEFGRIPVEWLNSKAYAAERAKLIKPDAILNPIYP
                     GQAPSHGDTTYFTVADADGMMVSMIQSNYRGMGSGLSPDGLGFMFQDRGELFSLTDGH
                     PNVYAPGKRPFQTIIPGFAVRDGTPWLSFGVMGGDMQPQGQAQIISNMVDFGLDLQAA
                     GDSPRWHHEGGSEPTGEALGQPGLLRLETGVPEATKKALAALGWPLGASDGGFGGYQA
                     IERWPGRYAAATEMRKDGVALAY"
     gene            1609..1956
                     /locus_tag="PLO65_06405"
     CDS             1609..1956
                     /locus_tag="PLO65_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRD27913.1"
                     /translation="MTRDEFHAIVMAFPGVEEAPSYGQPSYKVNGKFLTRVRKDDASA
                     VIMEVSFDERDLLLEVEPETFHFTAHYKDYPCVLARLETLSPDQLKGFLERRWRKAAK
                     KAEVKAWEAAKVE"
     gene            complement(1943..2323)
                     /locus_tag="PLO65_06410"
     CDS             complement(1943..2323)
                     /locus_tag="PLO65_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD27914.1"
                     /translation="MSGLILDSNALIWWLEDAPRIRRSSAAERIATDPLVMVSIVTPW
                     ELWIKAASGRLGLPGRFNERLPDLGLEIISPTLRDARLATALPPIHKDPFDRMIVAQA
                     LNRDAAIVTSDRKLRDYGVDVILL"
     gene            complement(2320..2601)
                     /locus_tag="PLO65_06415"
     CDS             complement(2320..2601)
                     /locus_tag="PLO65_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRD27915.1"
                     /translation="MKQVNVHQAKTQLSQLLADVEAGEVIVIARAGKPVARLTAEPAE
                     RKVLRQPGAMKGEFVWDEEAWKAGDAEILEMFEESINAPLFPPEPEEAS"
     gene            2698..4227
                     /locus_tag="PLO65_06420"
     CDS             2698..4227
                     /locus_tag="PLO65_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRD27916.1"
                     /translation="MAYRSLRDFIARLEADGELVRVTEPVSTVLEMTEICTRLLATGG
                     PAVLFEKPVMEDGTISPMPCLANLFGTVKRVALGVTLGGEPRTTAGELREVGELLAFL
                     RQPEPPRGIKDAMELLPLAQTVMSMRPKTVKSAPVQQVVLKGDQVDLGKLPVQTCWPG
                     EPAPLITWPLVVTKGPSDSKEDDFNLGIYRMQVLGKDRAIMRWLAHRGGAQHYGRHKK
                     AGRRDPLPACAVIGADPGVILAAVTPVPDTLSEYQFAGLLRGSKVDLVPAKTVPLMVP
                     AQAEIVIEGHVMIDEFEDEGPYGDHTGYYNSVERFPVFRVSAITMRRDAIYHTTFTGR
                     PPDEPSVLGEALNEVFIPLFQQQFPEVVDFWLPPEGCSYRIAVVSMKKAYPGHAKRVM
                     LGVWSYLRQFMYTKWVIVVDDDIDARDWKDVMWALSTRMDPARDITVIENTPIDYLDF
                     ASPESGLGSKIGLDATNKLPPETHREWGEKIRMDQAVVDRVSDMWGKLGLPGDGKPIW
                     K"
     gene            4247..6049
                     /locus_tag="PLO65_06425"
     CDS             4247..6049
                     /locus_tag="PLO65_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRD27917.1"
                     /translation="MFLAAIAAAVACGGAAMAAPWETDFLPPAIAWHGKSERLIVKAD
                     DPWITPSEMTGLTATPSYDETVAWLKRLDAASPLIRMEVFGKTPEGRDLYVVIASKDP
                     DRLDADKPVVLAQAGIHSGEIDGKDAGLMLLRDIAFKGKDGLLDGANFVFVPIFSADA
                     HERTSPWSRPNQRGPVSQGWRTTAQNLNLNRDYGKLDAPEMQAMMGLINRFDPALYFD
                     LHVTDGIDYQYDVTFGFEGEDGTFTRSPAIAGWLNAALKPAAYAALKKAGHIPGPLVF
                     AVDDRAPRTGLFIGPTGLRLSNGYGDARHMPTILVENHSLKPYNQRVLGMYVLLESAL
                     KSVAADTAGLKAAIAADRARRPAEVLTNWTPSTTPVGTRAFLGVAYETYRSPVSGRDE
                     VRWLGRPERTPWQEPLFSATAGLRLTPPAAYWAPATRPEIIARLRLQGIQMETLAAPR
                     TVTLSMARLPAAKIAGRTNEGHVGMEPGEAVWETRTVTMPAGSVRVPTDQPLGELAVL
                     LLEPESDEGFLAWGFFPEILQRTEYIEGYVIAPLGQRMLDADPALKAEFEAKLAADPA
                     FAADPDARLGWFYERTPYFDDHYRLYPVGREMGH"
     gene            6125..6526
                     /locus_tag="PLO65_06430"
     CDS             6125..6526
                     /locus_tag="PLO65_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD27918.1"
                     /translation="MDVVNPGHASALWVGLHILLMLVLSLLVVRLRNKHKVFLGDEGI
                     PELAQAIRAFGNASEYVPPGLAGLIALDLSGATPLLIHLAGFILLVGRVVHAVGLSNS
                     VGTSIPRALGMTMTWCAYVFLVAALLISAVW"
     gene            6639..>7042
                     /locus_tag="PLO65_06435"
     CDS             6639..>7042
                     /locus_tag="PLO65_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRD27919.1"
                     /translation="MDETLLHEAVADALKAGADAAEAVFAERQSLSVNVRMGELEEVE
                     REESRDLGLRVFIGQRQAVVSGSDVSAEGRRKLIERVVAMARLAPEDPYAGFAPADRL
                     ARGPFADLDLFDAAEPSPETLEDQAREAEAAAR"
BASE COUNT         1120 a         2438 c         2403 g         1081 t
ORIGIN      
        1 cggggcggcg gcgacggccc atccgctggc caccctggcg gcgatcgaca tcctgcggcg
       61 cggcgggtcg gccgtcgacg cggccatcgc ggccaatgcc tgcctgggcc tcctcgaacc
      121 gatctcgagc ggcatcggcg gcgattgttt cgtcatgctg tgggacccga agacgaagaa
      181 ggtcgtcggc ctcaatggct cgggccgctc cccgcgcggc ctgacgctgg agacccagcg
      241 cgcgcggtcg aagaacggcc atatcgccag cttcggggcc atctcggtca gcgtgcccgg
      301 cgcggtccag gcctggtggg acctgcacgg caagttcggc aagctgccgt ggaaggacct
      361 gttcggcccg gccatcggct acgctgacga ggggacgccg gtcacccaga acgtcgccta
      421 ctacctggcc gcctcgttcc gccggttcaa caacccggcc tcgaacatcg aggaggtggt
      481 caacttcaac aaggtctggg cggccgaggg gcgcacgccg cgcgagggcg agctgttccg
      541 caatccggcc ctggcctcga cctatcgcgc gatcgccgcc ggcggccgcg acgccttcta
      601 cgacggcgag atcgccgatc gcatcgaggc ctacttcaag cgcatcggcg gctggatgac
      661 ccgcgccgac ctggccgctc accacagcga atggaccacc ccgctggtca ccggctaccg
      721 cggcgtcgac gtctacggcc tggcccccaa cagccaggga ctgtcgaccc tgcagctgct
      781 gaacatcctg gagacgttcg acgtcaaggc gatgggcttc cagtcggccc aggcgatcca
      841 ccatgcggtc gaggccaagc ggctggccta cgaggaccgc gcccgctact tcgccgaccc
      901 cgaattcggc aggatccccg tcgagtggct caactccaag gcctacgcgg cggagcgggc
      961 gaagctgatc aagccggacg cgatcctcaa cccgatctat ccgggccagg ccccgagcca
     1021 tggcgacacg acgtatttta ccgtcgccga cgctgacggc atgatggtct cgatgatcca
     1081 gtccaactac cgcggcatgg gcagcggcct gtcgccggac ggcctgggct tcatgttcca
     1141 ggaccggggc gagctgttct cgctgaccga cggccatccc aacgtctatg cgcccggcaa
     1201 gcggccgttc cagaccatca tcccgggctt cgcggtgcgg gacgggacgc cctggctgag
     1261 cttcggcgtg atgggcggcg acatgcagcc ccagggccag gcccagatca tcagcaacat
     1321 ggtcgatttc ggcctcgacc tgcaggcggc cggcgacagc ccgcgctggc accatgaggg
     1381 cggcagcgag ccgaccggcg aggcgctggg ccagcccggc ctgttgcggc tcgagaccgg
     1441 cgtgcccgag gcgacgaaaa aggccctggc cgccctcggc tggccgctcg gcgcgtcgga
     1501 cggcggcttc ggcggctacc aggcgatcga gcgctggccc ggccgctacg ccgccgccac
     1561 agagatgcgc aaggatggcg tggcgctggc ctactgacgg agcgcatcat gacccgcgac
     1621 gagttccacg ccatcgtcat ggccttcccc ggcgtcgagg aggcgcccag ctacggccag
     1681 ccgtcctaca aggtgaatgg caagttcctc acccgcgtgc gcaaggacga cgccagcgcg
     1741 gtgatcatgg aggtgtcgtt cgacgagcgc gacctgctgc tcgaggtcga gccggagacc
     1801 ttccacttca ccgcccatta caaggactac ccctgcgtgc tggcgcggct ggagacgctc
     1861 agtcccgacc agctgaaggg cttcctcgaa cgccgctggc gcaaggcggc gaagaaggcc
     1921 gaggtgaagg cctgggaggc ggctaaagta gaatgacgtc cacgccatag tcgcggagct
     1981 ttctgtcgct ggtgacaatc gccgcgtctc gattcagcgc ctgggccacg atcatgcggt
     2041 cgaacgggtc cttgtgaatc ggcggcaggg cggtggcgag ccgcgcgtcg cggagggtcg
     2101 gcgagatgat ctcgagaccg aggtcgggca gccgctcgtt gaatcgcccg ggcagcccta
     2161 gccgtccgga agcggccttg atccacagtt cccagggcgt gacgatgctg accatcacca
     2221 ggggatcggt ggcgatccgt tccgctgcgc tggatcgtct gatccgtggc gcgtcctcaa
     2281 gccaccagat cagggcgttg ctgtcgagga tcaggccgct caagacgcct cttcaggctc
     2341 gggcggaaac agcggcgcgt tgatcgactc ctcgaacatc tcgaggattt ccgcgtcgcc
     2401 ggccttccac gcctcttcat cccagacgaa ttctcccttc atcgcgccgg gctggcgaag
     2461 caccttgcgc tcagcgggct ccgccgtaag tcgcgcgacg ggcttgccgg ccctggcgat
     2521 cacgatgacc tcaccggcct ccacgtcggc gaggagctgg gagagctggg tcttggcctg
     2581 atgaacgttg acctgcttca tgtggactaa cttagtccac ttgggtcgaa acgccaaggc
     2641 gtgacacggc ggccccgccg ccctagcttc cgccgcgcgc aacgggagca ctgaaccttg
     2701 gcctatcggt cgctgcggga cttcatcgcc aggctggaag cggacggcga actggtgcgg
     2761 gtgaccgagc cggtgtcgac cgtgctggag atgaccgaga tctgcacccg gctgctggcc
     2821 accggcgggc cggcggtgtt gttcgaaaag ccggtgatgg aagacggcac gatctcgccg
     2881 atgccctgcc tggccaacct gttcggcacc gtgaagcggg tggcgctggg ggtgacgctc
     2941 ggcggcgagc cgcgcaccac ggcgggcgag ctgcgcgagg tcggcgagct gctggccttc
     3001 ctgcgccagc ccgagccgcc gcgtggcatc aaggacgcca tggagttgct gccgctggcc
     3061 cagaccgtga tgtcgatgcg gccgaagacc gtgaagtccg cgcccgtgca gcaggtggtg
     3121 ctcaagggcg accaggtcga cctcggcaag ctgccggtcc agacctgctg gccgggcgag
     3181 cccgcgccgc tgatcacctg gccgctggtg gtgacgaagg ggccgtcgga ctcgaaggag
     3241 gatgacttca acctcggcat ctaccgcatg caggtgctgg gcaaggaccg ggcgatcatg
     3301 cgctggctgg cccaccgcgg cggggcccag cactacggcc gccacaagaa ggccggtcgc
     3361 cgcgacccgc tgcccgcctg cgcggtgatc ggcgccgatc cgggcgtgat cctcgcggcc
     3421 gtgacgccgg tcccggacac cctgtcggaa taccagttcg ccggcctgct gcgcgggtcc
     3481 aaggtcgacc tcgtgccggc gaagaccgtg ccgctgatgg tcccggccca ggccgagatc
     3541 gtcatcgagg gccatgtgat gatcgacgag ttcgaggacg agggccccta cggcgaccac
     3601 accggctact acaacagcgt cgagcgcttc ccggtgttca gggtcagcgc gatcaccatg
     3661 cgcagggacg ccatctacca caccaccttc accggccggc cgccggacga gccgtcggtg
     3721 ctcggcgagg cgctcaacga ggtgttcatc ccgctgttcc agcagcagtt tcccgaggtg
     3781 gtcgacttct ggctgccgcc ggagggctgc agctaccgca tcgccgtggt gtcgatgaag
     3841 aaggcctacc ccggccacgc caagcgggtg atgctcggcg tctggagcta cctgcgccag
     3901 ttcatgtaca ccaagtgggt catcgtcgtg gatgacgaca tcgacgcccg cgactggaag
     3961 gacgtcatgt gggcgctcag cacccgcatg gatccggcgc gcgacatcac ggtgatcgag
     4021 aacaccccga tcgactacct cgacttcgcc agccccgaga gcggcctcgg cagcaagatc
     4081 ggcctcgatg cgaccaacaa gctgcccccc gagacccacc gcgaatgggg ggagaagatc
     4141 cgcatggacc aggcggtcgt cgaccgggtg tcggacatgt ggggcaaact gggcctgccc
     4201 ggggacggca agccgatctg gaaatgaggt tcgcgcgttg aacaggatgt tccttgcggc
     4261 gatcgccgcc gccgtggcct gtggaggcgc cgccatggcc gctccctggg agaccgactt
     4321 cctgccgccg gccattgcct ggcacggcaa gagcgagcgg ctgatcgtca aggcggacga
     4381 tccgtggatc acgccctcgg aaatgaccgg cctgacggcc acccccagct acgacgagac
     4441 cgtcgcctgg ctgaagcggc tcgacgccgc ctcgccgctg atccgcatgg aggtgttcgg
     4501 caagacgccg gaagggcgcg acctctacgt ggtcatcgcc agcaaggacc ctgaccggct
     4561 cgatgccgac aagccggtgg tgctggccca ggccggtatc cacagcggcg agatcgacgg
     4621 caaggacgcc ggcctgatgc tgctgcggga catcgccttc aagggcaagg acggcctgct
     4681 ggacggggcc aacttcgtct tcgtgccgat cttcagcgcc gacgcccatg agcgaaccag
     4741 tccctggagc cgccccaacc agcgtggccc ggttagccag ggctggcgga cgacggccca
     4801 gaacctcaat ctcaaccgcg actacggcaa gctcgacgcg ccggagatgc aggccatgat
     4861 gggcctgatc aaccgcttcg acccggcgct ctatttcgac ctgcatgtca ccgacggcat
     4921 cgactaccag tacgacgtga ccttcggctt cgagggcgag gatggaacct tcacccgctc
     4981 gcccgccatc gccggatggc tgaacgcggc cctcaagccg gcggcctatg cggcgttgaa
     5041 gaaggccggc cacatcccgg gaccgctggt gttcgccgtc gatgaccggg cgccgaggac
     5101 cggcctgttc atcgggccga cggggctgcg gctgtcgaac ggctatggcg acgcgcgcca
     5161 catgccgacg atccttgtcg agaaccactc cctcaagccc tacaaccagc gggttctggg
     5221 catgtacgtg ctgctggagt cggcgctgaa gtcggtggcg gccgacaccg ccggcctgaa
     5281 ggccgccatc gccgccgacc gggcccgccg gccggccgag gtgctgacca actggacccc
     5341 gtcgaccacg cccgtgggga cccgcgcctt cctcggcgtg gcttacgaga cctatcgctc
     5401 gccggtctcg ggccgcgatg aggtgcgctg gcttggccgg cccgagcgga cgccctggca
     5461 ggagccgctg ttctccgcca cggccgggtt gcggctgact ccgcccgccg cctactgggc
     5521 gcccgcgacc aggcccgaga tcatcgcccg cctgcgtctg cagggcatcc agatggaaac
     5581 cctggccgcg ccgaggacgg tgaccctgtc gatggcgcgc ctgccggccg cgaagatcgc
     5641 cggccggacc aacgaaggcc atgtcggcat ggagcctggc gaggcggtct gggagacccg
     5701 caccgtcacc atgcccgcgg gctccgtccg cgtaccgacc gaccagccgc tcggcgaact
     5761 ggccgtgctg ctgctggaac ccgagagcga cgagggcttc ctcgcctggg gcttcttccc
     5821 ggagatcctg cagcgcaccg agtatatcga gggctatgtc atcgcgccgc tgggccagcg
     5881 gatgctcgat gccgatccgg ccctgaaggc cgaattcgag gcgaagctgg ccgccgaccc
     5941 ggccttcgcc gccgatccgg acgcgcggct cggctggttc tatgaacgga cgccctattt
     6001 tgacgatcat taccggcttt atccggtggg acgcgaaatg ggccattgag cggccttgcc
     6061 ctcgacgggc aggcacggtt gattgcgccg cgctgaaacg gtgatttttg aagaaggacg
     6121 cgggatggac gtcgtcaatc cgggccacgc ctcggccctg tgggtcgggt tgcacatcct
     6181 gctgatgctg gtgctttcgc tgctggtcgt gcggctgcgc aacaagcaca aggtattcct
     6241 cggcgacgag ggcatcccgg aactggccca ggccatccgc gccttcggca atgccagtga
     6301 atacgtgccg ccgggcctgg ccgggctgat cgcgctggac ctgtcgggcg ccacgccgct
     6361 gctgatccat ctcgccggct tcatcctgct ggtcggcagg gtggtgcacg ccgtgggcct
     6421 gtcgaacagc gtcggcacct cgatcccccg ggcgctgggc atgaccatga cctggtgcgc
     6481 ctatgtcttc ctcgtcgccg ccctgctgat ttcggcggtg tggtgaagca gggacatgca
     6541 cttcagtgcg tgtccctttc cggccggagg ggacacgcac agaagtgcat gtcccctgag
     6601 ccttgccgcg acgcccgggc gtccccataa cccggggcat ggatgaaacc ctgttgcatg
     6661 aagcggtcgc cgacgccctg aaggccggcg ccgacgcggc cgaggctgtc ttcgccgaac
     6721 gccagtccct gtcggtcaac gtccggatgg gcgagctgga agaggtcgag cgcgaggaat
     6781 cgcgcgacct cggccttcgc gtgttcatcg gccagcgcca ggccgtggtg tccggctctg
     6841 atgtctcggc cgagggccgc cgcaagctga tcgagcgggt cgtggccatg gcccgcctcg
     6901 cgcccgagga tccctacgcc ggcttcgcgc cggccgaccg gctggcccgg gggccgtttg
     6961 ccgacctcga cctgttcgac gccgccgaac cctcgcccga gaccctcgag gaccaggccc
     7021 gcgaggccga ggccgccgcg cg
//
LOCUS       DAORNX010000110         7031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7938_length_7031_cov_6.669725, whole genome shotgun sequence.
ACCESSION   DAORNX010000110 DAORNX010000000
VERSION     DAORNX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7938_length_7031_cov_6.669725"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLO65_06440"
     CDS             <1..935
                     /locus_tag="PLO65_06440"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRD27920.1"
                     /translation="NLVAASFGLGLKAGDEIVLTQMEHHSNIVPWHLLRERKGVVLRF
                     APVREDGSLDLDGLKSLIGERTRLVAVTQMSNVLGTINPVRAIADLAHAAHAAGAAVL
                     VDGCQGAVHLAVDVKALDADFFVCTGHKLYGPTGVGVLYGKAERLAALPPYQAGGEMI
                     GTVTEARITYADPPMRFEAGTPPILEAIALGAALEWLAGFDREAVAAHERALYDRVVE
                     RLDGANWLRILGTTPDKGAILSFTVDGAHAHDIAQILDRQGVAVRAGTHCAEPLMARF
                     GVTSSARASFALYNTLAEADAFVDALVKARSFFV"
     gene            940..1302
                     /locus_tag="PLO65_06445"
     CDS             940..1302
                     /locus_tag="PLO65_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRD27921.1"
                     /translation="MTDTATPVTETPSAIPQAELDALTDQLVEKLKTVFDPEIPVDIY
                     ELGLIYKVDVSDDREVKIDMTLTAPGCPVAGEMPGWVEDAVMEIPGLKSCSVDLVFDP
                     PWDPSRMSDEAKLQLNMF"
     gene            1305..1700
                     /locus_tag="PLO65_06450"
     CDS             1305..1700
                     /locus_tag="PLO65_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRD27922.1"
                     /translation="METVQITPRPRRPRPKVVTLTDAAADRVKEIMARADKPYAGLRV
                     GVKNGGCAGSEYIIEYAEKADPLDEVVEDHGVTILIEPKAVLFLIGAEIDYEISRLSS
                     KFVFRNPNETDACGCGESVTIQPATDHEA"
     gene            1793..2215
                     /locus_tag="PLO65_06455"
     CDS             1793..2215
                     /locus_tag="PLO65_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27923.1"
                     /translation="MVHLIVSAAAAVILVAGAPADPFADVNPDHFEGLARDDLAAWSY
                     GWNTDKVSGSVVTVLMQARYNTPTPFNGNPTPVAYSIHKIQLDCAARTVTYLEGSNYT
                     ASGAFVGPGSPSAARPWTDGTTGFQAFAAEMCSSDWKR"
     gene            complement(2223..3017)
                     /locus_tag="PLO65_06460"
     CDS             complement(2223..3017)
                     /locus_tag="PLO65_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD27924.1"
                     /translation="MTFRNRLFNAYYWVLSIFYGLSAALLALAPGRAPMTAAIRLYTR
                     RMLWAMDRLAGIRIELKGQDRLPDGAFIIAAKHHSWGDGFVMFANVDNLSFVTGDHME
                     RFPLVGAILRKFGAIIVDNCGGPEARRALSESAAQVAAEGRRILIYPEGNLAKPGERF
                     RYRTGVYYMSRDFNLPVVPVATNLGCFWEQTSKIKRPGTATIEFLDPIAVGLDRTPFL
                     ERLESVIETRSQELIAMATGEPVRPSLLVPTPDEARKAAAATVAAA"
     gene            complement(3027..3587)
                     /locus_tag="PLO65_06465"
     CDS             complement(3027..3587)
                     /locus_tag="PLO65_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD27925.1"
                     /translation="MSAKPRIERAALEVFVRDGVDAATTRAIAAAAGVSEGALYRHYK
                     GKDELAVSLFMTTHRRLSSLIEASAAQAVGIRDKAAAIVAAYCQVADEDWLTFSFHLT
                     QIHRFVPVYQEDGRDPVTVTENVIKQAMIAAEIPPADPRVLAAMAIGVITQTAQNKVY
                     GRLGADPLSAHAGLMTAGVQALLFAR"
     gene            3669..4064
                     /locus_tag="PLO65_06470"
     CDS             3669..4064
                     /locus_tag="PLO65_06470"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRD27926.1"
                     /translation="MAVSRPFLDHLSELLAGLGPLTVKTMFGGASLQVDGAAFALVFG
                     ETLYLKVDDENRAAFEAEGAGPFVYAMKDGRKASLGYWSLPERALDDPDEAVRWARLG
                     VEAAFRAKRSKKKKGRATRDDIGPGPWDG"
     gene            complement(4093..4896)
                     /locus_tag="PLO65_06475"
     CDS             complement(4093..4896)
                     /locus_tag="PLO65_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase"
                     /protein_id="HRD27927.1"
                     /translation="MDYKKIKVELVDGVAVVTMSDPATMNAAGLDLAGELSHALSSIA
                     AGKVVARAVVLTGEGRGFCSGANLGGGGAAGRELDVDGKPDAGRALETVYNPLVSQMR
                     DLPVPLVTAVNGAAAGVGCSFALLGDLIVAAESAYFLQAFRRIGLVPDGGSTYLLPRA
                     VGRARAMEMALLGEKIGAAKALEWGMVNRVVPDAELRGAAIALASELANGPASLGAIR
                     RLIWDSLDGDWNGQLTAERKAQKIAGKTEDFIEGVSAFLQKRVAAFKGR"
     gene            complement(4971..6080)
                     /locus_tag="PLO65_06480"
     CDS             complement(4971..6080)
                     /locus_tag="PLO65_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27928.1"
                     /translation="MNTHYALQAFAQAAGGIFFLVFLLRAGVEVPFALLFQAGVVAAR
                     FMLRPAILPLAVRWGVKPLLIAGTLLTATDYPLLALVEGPGPYLVLLCAVSSLGYLLY
                     WLSFHAYFAALGEAADRGGQIGLREAIVALVGIVAPLLGALGLTTLGPTPTFWAVGLV
                     QALAIVPLIGAPNVSVPAAVPAAATPAWTGLGLMVGDGLFAATYHYVWLIALFLALGQ
                     SYSAYGGAAAAAAVVGAFSGLVLGRHVDSGHGRRSAILAYSAAALVVILRAASLGLPW
                     LAVLANAAGSLLVTIVTPVLMTPVYNLAKASPCVFRFHMATEGAWDVGCFLGCVAAAA
                     LAAAGLPLSVPLLLALAPAAMLALQLTRYYGRGPA"
     gene            complement(6307..>7031)
                     /locus_tag="PLO65_06485"
     CDS             complement(6307..>7031)
                     /locus_tag="PLO65_06485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRD27929.1"
                     /translation="NEAYGQTLAGASKGLTDAEDVGQIRAMVDSLSSATRRVQKENKS
                     LEKRLAESTSEVQRLREHLEQVRRDATTDGLTNLANRRAFDEELARACAEADETGSIL
                     TLAVLDIDHFKSFNDTWGHQTGDQVIRYVASVIGRLGATPRFSARYGGEEFAMIFPGE
                     EAALVEATLQEIREEVASRMLKRRSTNEDLGTVTISAGLATRRDGETGACVMERADEA
                     LYASKRTGRNRVTNGETLAAAA"
BASE COUNT         1121 a         2366 c         2491 g         1053 t
ORIGIN      
        1 tcaacctggt cgcggcctcg ttcggcctgg gcctgaaggc cggcgacgag atcgtcctca
       61 cccagatgga gcaccactcc aacatcgtgc cctggcatct gctgcgcgag cgcaagggcg
      121 tggtgctgcg gttcgcgccg gtgcgcgagg atggttcgct cgacctggac gggttgaagt
      181 ccctgatcgg cgaaaggacc aggctggtcg ccgtgaccca gatgtccaac gtgctgggaa
      241 cgatcaaccc ggtccgcgcc atcgccgacc tggcgcatgc ggcgcatgcg gcgggtgctg
      301 cggtgctggt cgacggctgc cagggcgcgg tgcacctggc cgtggatgtg aaggctctgg
      361 acgccgactt cttcgtctgc acgggccaca agctttacgg tccgaccggc gtgggcgtgc
      421 tctacggcaa ggccgaacgg ctggcggcct tgccacccta ccaggccggc ggcgagatga
      481 tcggcacggt gaccgaagcc cgcatcacct acgccgaccc accgatgcgg ttcgaggccg
      541 gcaccccgcc gatcctcgag gccatcgcgc tgggcgcggc gctggaatgg cttgcgggct
      601 tcgatcgcga ggccgtcgcc gcgcacgaac gcgccctcta cgaccgggtg gtcgagcggc
      661 tcgacggcgc caactggctg cgcatcctcg gcacgacgcc cgacaagggc gcgatcctca
      721 gcttcaccgt cgacggggcc cacgcccacg acatcgccca gatcctcgac cggcaggggg
      781 tggcggtccg cgccggaacc cactgcgccg agcccctgat ggcccgtttc ggcgtcacgt
      841 cgagcgcccg ggcctcgttc gccctatata acaccctggc cgaagccgac gcgttcgtgg
      901 acgcgctggt caaggcccgc agcttcttcg tctgagatca tgaccgacac agcgacgccc
      961 gtgaccgaaa cgccctccgc cattccgcag gcggagctcg acgcgctgac cgatcagctg
     1021 gtggagaagc tcaagaccgt gttcgacccg gagatcccgg tcgacatcta cgaactgggc
     1081 ctcatctaca aggtcgacgt ctcggacgat cgcgaggtga agatcgacat gaccctgacc
     1141 gcgcccggtt gcccggtggc gggggaaatg ccgggctggg tcgaggacgc ggtaatggaa
     1201 attcctggcc tgaagtcctg ctccgtcgac ctggtcttcg acccgccgtg ggacccctcg
     1261 cgcatgagcg acgaggccaa gctgcagctg aacatgttct gaccatggaa accgttcaaa
     1321 tcaccccccg tccgcgccgc ccgcgcccca aggtcgtcac cctgaccgac gccgcggccg
     1381 accgcgtcaa ggaaatcatg gcccgggccg acaagcccta cgccggcctg cgtgtgggcg
     1441 tgaagaacgg tggctgcgcc gggtccgaat acatcatcga gtatgccgaa aaggccgatc
     1501 ctctggacga ggtggtcgag gatcacggcg tgaccatcct gatcgagccc aaggccgtgc
     1561 tgttcctgat cggcgccgag atcgactacg agatcagccg gctgagttcg aagttcgtct
     1621 tccgcaatcc gaacgagacc gacgcctgcg gctgcggcga gagcgtcacc atccagcctg
     1681 cgaccgacca cgaggcctga ccgccgattt ccgcgatctg ggtaggtcgc gaattgtcac
     1741 ccccgcggcc cggtgaaatc gtcgcctctt cctgaggggg aggctgacaa caatggtcca
     1801 tctgattgtg tccgccgcgg cggcggtcat cctggtcgcc ggcgcgccgg ctgatccgtt
     1861 cgccgacgtc aatccagacc atttcgaagg gctggcgcgc gacgatctcg ccgcctggtc
     1921 ctacggctgg aacacagaca aggtgtcagg ttcggtcgta accgtgctga tgcaggcccg
     1981 ctacaacacg ccgacgccgt tcaacggcaa tccgacgccc gtggcctatt cgatccacaa
     2041 gatccagctc gactgcgccg cccggacggt gacctatctc gagggctcga actacacggc
     2101 cagcggggcc tttgtcggac ccggctcgcc cagcgccgcc cggccctgga cagacgggac
     2161 gaccggcttc caggcgttcg cggcggagat gtgctcgagc gactggaaga ggtaggccga
     2221 ggtcaggccg ccgccactgt cgccgccgcc gccttgcgcg cctcgtccgg cgtcgggacc
     2281 agcaggctgg gcctgacggg ctcgcccgtg gccatggcga tcaattcctg gctgcgggtc
     2341 tcgatgacgg actccaggcg ctccaggaac ggggtgcggt ccaggcccac cgcgatcggg
     2401 tcgaggaatt cgatggtggc ggtccccggg cgcttgatct tcgacgtctg ctcccagaag
     2461 cagcccaggt tggttgccac cggcaccacc ggcaggttga agtcgcggct catgtagtag
     2521 acgccggtgc ggtagcggaa ccgctcgcct ggcttcgcca ggttcccctc cggatagatc
     2581 aggatgcggc gaccctcggc ggccacctgg gcggcgcttt ccgacagggc cctgcgcgct
     2641 tcgggaccgc cgcagttgtc gacgatgatc gcgccgaact tgcgcaggat ggccccgacc
     2701 agcggaaacc gctccatgtg gtcgccggtc acgaacgaca ggttgtcgac gttggcgaac
     2761 atcacgaagc cgtcgcccca gctgtggtgc ttggccgcga tgatgaaggc tccgtcgggc
     2821 agtctgtcct ggcccttgag ctcgatcctg atgccggcca ggcggtccat cgcccacagc
     2881 atgcgacgcg tgtagaggcg gatcgcggcg gtcatcggcg cgcggccggg cgccagggcc
     2941 agcaatgcgg cgctgaggcc gtagaagatc gacagaaccc agtagtaggc gttgaacagc
     3001 cggttgcgga aggtcatcgg aagtccctag cgggcgaaga ggagggcctg gacgccggcg
     3061 gtcatcaggc cggcgtgggc ggagagagga tcggcgccca gacggccata gaccttgttc
     3121 tgggcggtct gggtgatcac accgatggcc atggcggcca gcacgcgcgg gtcggccggc
     3181 gggatctcgg cggcgatcat cgcctgcttg atgacgtttt cggtcacggt cacggggtcg
     3241 cggccgtctt cctggtagac cgggacgaag cggtggatct gggtcaggtg gaagctgaac
     3301 gtcagccagt cctcgtcggc cacctggcaa taggcggcga cgatggcggc ggccttgtcg
     3361 cgaatgccga ccgcctgcgc ggcgctggcc tcgatcaacg aagacagccg ccggtgggtg
     3421 gtcatgaaca ggctgacggc cagttcgtcc ttgcccttgt agtggcggta gagggcgcct
     3481 tcggaaaccc ctgccgccgc ggcgatggcg cgggtggtgg ccgcgtcgac gccatcgcga
     3541 acgaagactt ccagggcggc gcgctcgatg cgaggtttgg cggacattgg cgggcgactc
     3601 cgcgtgtgtg agcatttgct cacatcgcgg acagcttcgc aagtgaacaa tcactcacac
     3661 ggtgggccat ggccgtcagt cgcccgtttc tcgatcatct gtcggaactg ctggcggggc
     3721 tgggacccct gacggtgaag acgatgtttg gcggcgccag cctgcaggtc gatggcgcgg
     3781 ccttcgccct ggtgtttggc gagacgctgt atctgaaggt cgatgacgag aaccgcgccg
     3841 ccttcgaggc ggagggcgca gggccgttcg tctacgccat gaaggacgga cgcaaggcct
     3901 cgctgggcta ctggagcctg cccgagcgcg cgctggatga tccggacgag gccgtgcgct
     3961 gggctcggct gggcgtcgag gcggcgttcc gggcgaagag atcgaaaaag aagaagggcc
     4021 gggcgacgcg cgacgacatc gggcccggcc cctgggacgg gtgacggact tccttctccc
     4081 gccgggagag ggctaccgcc ccttgaacgc cgcgacgcgc ttttgcagga aggcgctgac
     4141 gccttcgatg aagtcctcgg tcttgccggc gatcttctgc gccttgcgtt cggcggtcag
     4201 ctggccgttc cagtcgccgt cgaggctgtc ccagatcagg cgtcggatgg cgcccagcga
     4261 cgccggcccg ttggccagtt ccgacgccag ggcgatcgcc gcgccgcgca gctcggcgtc
     4321 cggcaccacg cggttgacca tgccccattc gagcgccttg gccgcgccga tcttctcgcc
     4381 cagcagggcc atttccatgg cccgggcccg cccgacggcg cgcggcagca ggtaggtcga
     4441 gccgccgtcc ggcaccaggc cgatgcgccg gaaggcctgc aggaagtaag cgctctcggc
     4501 ggccacaatc aggtcgccca gcagggcgaa agagcagccg acgccggcgg cggcgccgtt
     4561 gacggccgtc accagcggca ccggcaggtc gcgcatctgg ctgaccaggg ggttgtagac
     4621 ggtttccagc gcccgaccgg cgtccggctt gccgtcgacg tccagctcgc ggcccgccgc
     4681 gccgccgccg ccgaggttgg cccccgagca aaagccgcgg ccttcgccgg tgagcaccac
     4741 ggcgcgggcg acgaccttgc cggcggcgat gctggagagg gcgtggctca gttcaccggc
     4801 caggtccagg ccggccgcgt tcatcgtcgc ggggtcagac atggtcacca cggcgacgcc
     4861 gtcgaccagc tcaaccttga tcttcttgta gtccacccga gcgtcctccg agggtttcgt
     4921 gtgtgggctg gctagtcccc ataacgccgg ggaacgccag tcaaaatttt tcacgccggg
     4981 ccgcgaccgt agtagcgggt cagctgcagg gccagcatcg cggccggcgc aagggccagc
     5041 agcagcggaa cggacagggg caggccggcc gccgccaggg ccgcggccgc aacgcagccg
     5101 aggaagcagc caacgtccca cgccccttcg gtggccatgt ggaagcggaa cacgcagggc
     5161 gaggccttgg ccaggttgta taccggcgtc atcagcaccg gcgtgacgat cgtcaccagc
     5221 agggaccctg cggcgttggc cagcaccgcg agccagggca ggcccaggct cgccgcgcgc
     5281 aggatgacga cgagcgccgc ggccgagtag gcgaggatcg ccgaacggcg gccgtgacca
     5341 ctgtcgacat gccggcccag caccagcccg ctgaacgcgc cgacgacggc tgccgcggcg
     5401 gccgcgccgc cgtaggccga gtagctttgg ccgagggcca ggaacagggc gatcagccag
     5461 acatagtggt aggtcgcggc gaacaggccg tcgccgacca tcagccccag gcctgtccag
     5521 gccggcgtcg ccgccgccgg aacggccgcg ggaaccgaga cgttgggcgc gccgatcaac
     5581 gggacgatgg ccagggcctg gaccaggccc acggcccaga aggtcggcgt tggtccgagc
     5641 gtggtcaggc ccagagcccc cagcagcggc gcgacgatgc cgaccagggc gacaatcgcc
     5701 tcgcgcagcc cgatctggcc gccccggtcg gcggcctcgc caagggccgc gaaataggcg
     5761 tggaagctga gccagtagag cagatagccg agcgatgaca cggcgcagag caggacaagg
     5821 tagggaccgg gcccctcgac cagggccagc agcggatagt cggtcgcggt cagcagcgtt
     5881 ccggcgatca gcagcggctt gacgccccag cgcacggcta gcggcaggat cgccggccgc
     5941 agcatgaagc gcgccgcaac gacgcccgcc tgaaacagca gggcgaaggg aacctcgacc
     6001 cccgccctca acaggaagac gaggaagaag atgccgccgg cggcctgggc gaaggcctgc
     6061 agcgcatagt gggtgttcac gcgcgtgatc gcgcttgtcg ttctggagga catggagatc
     6121 cgaagggcgg ggggtgaacg catcgacgcc ccgcgatcgg gcgcggggcg gcgggatgga
     6181 cggctagcct tggctagcgg tcagcgtttg cgttcacaca tgagcggcgc gctccttcgg
     6241 gccggaggct cgcacgggat cggggcgggg agcaagccgt cccgggaccc cggggtcggg
     6301 cggcgatcag gccgccgccg cgagggtctc gccgttggtc acgcgattgc ggccggtgcg
     6361 cttggaggcg tagagcgcct cgtcggcccg ttccatgacg caggcgccgg tttcgccgtc
     6421 gcgacgggtg gccaggcccg cggagatggt caccgtgccc aggtcctcgt tggtcgaccg
     6481 gcgcttgagc atgcgcgagg ccacttcctc gcggatctcc tgcagggtcg cctcgaccag
     6541 ggcggcctcc tcgccgggga agatcatcgc gaattcctcg ccgccgtagc gggccgagaa
     6601 gcgcggcgtg gcgcccaggc gtccgatcac gctggcgacg tagcggatca cctggtcgcc
     6661 ggtctggtga ccccaggtgt cgttgaagct cttgaagtgg tcgatatcca gcaccgccag
     6721 ggtcaggatc gagccggtct cgtccgcctc ggcgcaggcg cgcgccagct cctcgtcgaa
     6781 ggcgcgccga ttggccaggt tggtcaggcc gtccgtcgtg gcgtcgcgtc gaacctgctc
     6841 gagatgctcg cgcaggcgct gcacctcgct ggtcgactcg gcgaggcgct tttcgagcga
     6901 cttgttttcc ttctgcacgc gacgggtggc ggaagacagg gaatcgacca tcgccctgat
     6961 ctgccccacg tcctcggcgt cggtcaggcc cttgctggcg ccggccagcg tctggccata
     7021 ggcctcgttc g
//
LOCUS       DAORNX010000111         7021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_7954_length_7021_cov_7.292995, whole genome shotgun sequence.
ACCESSION   DAORNX010000111 DAORNX010000000
VERSION     DAORNX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7954_length_7021_cov_7.292995"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(997..1425)
                     /locus_tag="PLO65_06490"
     CDS             complement(997..1425)
                     /locus_tag="PLO65_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRD27930.1"
                     /translation="MLVSQILKDKGDLVFTVGPTETVGAVAALLHSRRVGAMVILDGE
                     TVVGIVSERDIVRMVAEEGGAALARPIDVCMTRDVVFATLSETVDSLLGRMTDRRIRH
                     LPVMRDGRLAGIISIGDLVKHKISEIEAEADGLKAYIAAG"
     gene            complement(1488..2159)
                     /locus_tag="PLO65_06495"
     CDS             complement(1488..2159)
                     /locus_tag="PLO65_06495"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRD27931.1"
                     /translation="MSESQDDRAPADAAPPEPAFNAPAISLVLVLALATAYGVQVLLL
                     SPQAVSALALSAPALADGRWWTLISHVFLHGGLVHLLMNIGAALAFGPAVARYFGRGA
                     RSAGVFILYFLVCGVAGGMGFLALNPDSAAQVIGASGAISGLWGGAARLVGRRRGLWG
                     LWQGPAPGQIIAVVALNLLIAVTGFAAGGLQIAWQAHIAGFVAGLLLIGPFGALARPS
                     DRDRR"
     gene            2283..2708
                     /gene="hfaA"
                     /locus_tag="PLO65_06500"
     CDS             2283..2708
                     /gene="hfaA"
                     /locus_tag="PLO65_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HRD27932.1"
                     /translation="MAAFPSKTLPAAGALALLAGLAVAPAALAQQMSSNAASFNAGYG
                     RVSGQENRGVDYGTRDSSGNRLIVDGIIQSGETQSAFSRIDGGVFGGSTGVGAGGSST
                     AIGNNLVVITQGNWNTVIVTSVQTNTGDVTAGSTANGGD"
     gene            2762..3721
                     /gene="hfaB"
                     /locus_tag="PLO65_06505"
     CDS             2762..3721
                     /gene="hfaB"
                     /locus_tag="PLO65_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaB"
                     /protein_id="HRD27933.1"
                     /translation="MTACATPVARPNGLYANPIGTAPVTANPTPYTDALVCVAQYARQ
                     YNLRAPRISVGRISDLTGKEEADGSGRKVTQGASMMAMTAFAKAGIPQVERYDTSVSE
                     LELKYANNKLISDGPTPGVDGPVDPRKIFSGQVAGSDFYVTGGITELNFNIRSTGADV
                     LAGDTDATGPKGSFRSRIFVMNIALDLRLVNTRTLEVVDVISYQKQIIGREVGIGLFD
                     FLNGNVFDISGGTGALEPVQLAVRALIERATVEMAANLYGMPGPQACMNVDPLGGGTT
                     GATGGFTPAYNNLGTNNAATREDPRRWNDGRDTGDRAPLRGRY"
     gene            3675..4892
                     /gene="hfaD"
                     /locus_tag="PLO65_06510"
     CDS             3675..4892
                     /gene="hfaD"
                     /locus_tag="PLO65_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchor protein HfaD"
                     /protein_id="HRD27934.1"
                     /translation="MAVIPAIALLCAAGTSHGQTAPGTVVNNQIQLGDVFATQVIDVV
                     SATEGVSAQTAATGNVLVGSTEGVDLNIRSNQTMAGSAQATTTVNATGELGDSSQINT
                     IAIGNALEAGTTQATMVGVLTQIATASPQVTARGMLEADTAAAGDVGLATTAASNSVS
                     LGLTNGSAGVLVNQTNGASVLADGGAIIGYVSGTADVSGMASGNTMSLIGTDSSAART
                     VVYQSNDAALTQASQFTAYGTVQTASTSATASGNVAATANEGPLLDLTATQINQAYVR
                     AQAESSAYEFGEGAATAYGVGNSVLAGNSGDAVVLENVQLNTGGGVEAIASFAGADGY
                     DGYARATAVGNDATGYACSACNGSMAVDSRQTNSADVGATATTTIGSGRVINSTSTAV
                     GNNASFYVTRPGS"
     gene            5119..5745
                     /locus_tag="PLO65_06515"
     CDS             5119..5745
                     /locus_tag="PLO65_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27935.1"
                     /translation="MLSPWPWVGALVVYLLFRAWYDNWRGPLTAAEVAAFMARVEAGP
                     GAAHNDPAIVRAFLEADDGREFFMFNLVKIATGEAPHPETGAPTPRARLMRRYTRTFI
                     PALLARGGHPALAARKIAGYVDAWKVGPDPGWSIIGYMRYRSRRDMIAMATHPKFQGI
                     HDFKIAGTAETFSFPTRPMLMLLAPPRLWVALVLLLAAAFAQIALLVP"
     gene            5760..6773
                     /locus_tag="PLO65_06520"
     CDS             5760..6773
                     /locus_tag="PLO65_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD27936.1"
                     /translation="MDPVALSGAPGSPYSRKMLAVLRYRRIPYRLFWPGREPADYPSP
                     KVRLLPTFYLPGEDGALTAVTDSTPLIRRFERDYSDRLLRPASPALAFLDSLLEDYGD
                     EWLTKAMFHYRWAYRADIDQAAGVLPSWRGTLDDEMLAAMGKAFSERQIGRLRYVGSN
                     PTTGPVIEASYVRLLDLLEDHLKQHGFLLGGRPGAGDFGLYGQLTQLAAFDPTPMALA
                     ASRAPRVMAWVQLVEDLCGLEPADSDWFDPADLPDTLKAILAETGRVYAPLMAANARA
                     VMAGAETVETEIDGHPWSQNTFPYQAKCLAWLREEYAALPAGDRALVDAALAGTGWAG
                     MFG"
BASE COUNT         1144 a         2554 c         2278 g         1045 t
ORIGIN      
        1 ccagcgttcg ctctgagcca ggatcaaact ctcaggttga gttgaccgct aacccatcgt
       61 ttccaatctc ggggggaaac ccaagatcgg gcgtctggtt ttgcatagta tcttgacgag
      121 ttcccattca cattggccgc cgcgccgagg cgtggcgacc gatattcatg gtgtcttcaa
      181 gagaccgcaa ttgtcagcgt atcgaggata gacttgctct atccgcaaaa cgccgccgcc
      241 tgcgtttctc tttccaaatc aacgatgtca aagacccgac cgggttgccc ctgcccaccg
      301 cttagcgccc ggtgtcggcg aggagcggcg tatctagtcg ctccgttttt ccgtgtcaac
      361 cggcttcttt cgaagcattc cggtttctcc ggtttccgtc gttccggaat gaaccggggc
      421 ggcgggagag gcgtctctaa atgaaagcgc ccaagcaagt caaccagtcc ttttgatcgg
      481 aaccgatttt cttgaccggg aaaaatcgga aactttccga cccttccagg aggcggcctc
      541 aatactgaga agcctcgttc caagtcaatc cgctctattg aaaaacagaa ccgatcttct
      601 tggggcgccg ggcggaaacc ttccgttccg gcgaggagcg gcgcttctag tcgaagcccc
      661 atccctccgt caaccgacca ttttcaattt ccgccaactt ccggccgaag ccgtccgcag
      721 gacgcaaacc gggctcgggg ccgccgaggg gcttccctct tgagtaaccg tcaccgagtc
      781 gattgaggct gcggacccta gcggccaaac cggcgaaaat caagagcctt tggggcccag
      841 ggccttcgcg gccagctccg ggaatccgag cgagcgcggc gtatatcggg tgggcggccc
      901 gactcgcaac ccccgtcgac gacatttccg ccgtcgggcg gtcgcaacgg cgccgcgccg
      961 ggtggaggcg cgacacccgc cagggcgagg ccggggctat ccggcggcga tgtaagcctt
     1021 caggccatcg gcctcggcct cgatttcgct gatcttgtgc ttcaccaggt cgccgatcga
     1081 gatgatcccg gccagccgcc cgtcgcgcat caccgggaga tgacggatcc gccggtccgt
     1141 catgcggccg agcagggaat cgacggtctc agatagcgtg gcgaagacca catcgcgcgt
     1201 catgcagacg tcgatcgggc gggccagggc cgccccgccc tcctccgcca ccatccgcac
     1261 gatgtcgcgc tcggacacga tgccgaccac cgtctcgcca tcgaggatca ccatggcgcc
     1321 gacgcgccgg ctgtgcagca gggccgcgac cgccccgacc gtctcggtcg ggccgacggt
     1381 gaagacgagg tcgcccttgt ccttgagaat ctgggaaacg agcaaagctg gcacctccct
     1441 ctttatatac cgcgtcggcc gcaggctctc gcaatacccg ccgggaatca acgccggtcg
     1501 cggtcacttg gccgtgcgag ggcgccgaat ggcccgatca gcagaagacc ggcgacaaat
     1561 ccggcgatgt gggcctgcca ggcgatctgc aggccgccgg cggcgaagcc ggtgacggcg
     1621 atcagcaggt tgagagccac cacggcgata atctggcccg gcgcggggcc ctgccacaga
     1681 ccccaaaggc cccgccggcg gccgaccagc cgggccgccc cgccccacag gccgctgatc
     1741 gcgcctgagg cgccgatgac ctgggcggcg ctgtcggggt tcagcgccag gaaccccatc
     1801 ccgccggcga cgccgcaaac cagaaagtac aggatgaaca ccccggcgga ccgggcgccg
     1861 cgcccgaaat accgggccac cgccggaccg aaggccaggg ccgcgccgat gttcatcagc
     1921 agatgaacaa ggccgccatg cagaaagacg tggctgatca gcgtccacca gcgtccgtcg
     1981 gccagggccg gggcggacag ggccagggcc gacaccgcct gcggcgacag cagaaggacc
     2041 tgtacgccat aggcggtcgc cagggccagg acgaggacca ggctgatcgc cggggcgttg
     2101 aaagccggtt ccggcggcgc ggcgtcggcg ggggctcggt cgtcctgcga ttcgctcact
     2161 cgaattcctt ccgccttaac caattcgcca agggttgggc cgcaggatgg gcctgtcctg
     2221 ttttggcacg cgcattgctt gtgcgttcgc gtgcccttct taccccgcgc ggagcatttt
     2281 ccatggcagc gtttccgtcc aagacccttc ccgcggcggg cgccctggcc ctgctggccg
     2341 ggctggcggt cgcgccggcc gccctggccc agcagatgag cagcaacgcc gcctcgttca
     2401 atgccggcta tggccgggtt tccggtcagg agaaccgcgg cgtcgactac ggaacgcggg
     2461 attcgtcggg caaccggctg atcgtcgacg gcatcatcca gagcggcgag acccagagcg
     2521 ccttctcgcg catcgacggc ggggtgttcg gcggctcgac cggcgtcggc gcgggcggca
     2581 gctcgacggc catcggcaac aatctggtgg tgatcaccca gggcaactgg aacacggtga
     2641 tcgtcacctc ggtccagacc aataccggcg acgtcaccgc cggctcgacg gcgaacgggg
     2701 gcgactgatg tccagagccg gggcgcgcac cgccgcgctg cttaccgcgg cctccttcgc
     2761 catgacggcc tgcgccacgc ccgtggccag gccgaacggc ctctacgcca acccgatcgg
     2821 caccgcgccg gtgaccgcca acccgacgcc gtacaccgac gcgctggtct gcgtcgccca
     2881 gtacgcgcgc cagtacaatc tgcgggcccc gcgcatctcg gtcggccgga tcagcgacct
     2941 gaccggcaag gaagaggccg acggctccgg ccgcaaggtc acccagggcg cctcgatgat
     3001 ggccatgacg gccttcgcca aggccggcat cccgcaggtc gagcgctacg acacctcggt
     3061 gtcggagctt gagctgaaat acgccaacaa caagctgatc agcgacgggc ccacccccgg
     3121 cgtcgacggc ccggtcgatc cgcgcaagat cttctcgggc caggtggcgg gatcggactt
     3181 ctacgtcacc ggcggcatca ccgagctgaa cttcaacatc cgctcgaccg gggccgacgt
     3241 gctggccggc gataccgacg ccaccgggcc caagggcagc ttccgcagcc gcatcttcgt
     3301 gatgaacatc gccctcgacc tgcggctggt gaacacccgc acgctcgagg tggtcgacgt
     3361 catctcctac cagaagcaga tcatcggccg tgaagtcggc atcggcctgt tcgacttcct
     3421 gaacggcaac gtgttcgaca tttcaggcgg cacgggcgcc ctcgagccgg tccagctggc
     3481 ggtgcgggcg ctgatcgagc gcgccacggt cgagatggcc gccaacctgt acggcatgcc
     3541 cggcccgcag gcctgcatga atgtcgatcc gctggggggc ggcaccaccg gcgcaacggg
     3601 cggcttcacg cccgcctaca acaatctggg aaccaacaat gccgcgaccc gcgaagaccc
     3661 tcgtcgctgg aacgatggcc gtgataccgg cgatcgcgct cctctgcgcg gccggtacta
     3721 gccacggcca gacggcgccc ggcacggtcg tcaacaacca gatccagctg ggcgacgtgt
     3781 tcgccaccca ggtgatcgac gtggtctcgg cgaccgaggg cgtcagcgcc cagacggccg
     3841 ccaccggcaa cgtgctggtc ggctccacgg agggcgtcga cctcaacatc cgctccaacc
     3901 agaccatggc cgggtcggcc caggccacga ccaccgtcaa cgccaccggc gagctgggcg
     3961 acagttcgca gatcaacacc atcgccatcg gcaacgccct cgaggccggc acgacccagg
     4021 ccaccatggt cggggtgttg acccagatcg ccaccgcctc gcctcaggtc accgcccgcg
     4081 gcatgctcga ggccgacacg gccgcggccg gcgacgtcgg actggccacc accgccgcgt
     4141 ccaacagcgt ctcgctgggc ctgaccaacg gctcggccgg cgtgctggtc aaccagacca
     4201 acggcgcctc ggtgctggcc gacggcggcg ccatcatcgg ctacgtcagc gggacggccg
     4261 acgtctccgg catggcctcc ggcaacacca tgagcctgat cgggaccgac tcctcagccg
     4321 cgcgcacggt ggtctaccag agcaacgacg ccgccctcac ccaggccagc cagttcaccg
     4381 cctacggcac ggtccagacc gccagcacca gcgccaccgc cagcggcaat gtggcggcca
     4441 ccgccaacga gggaccgctg ctggacctga ccgcgaccca gatcaaccag gcctatgtcc
     4501 gggcccaggc cgaaagcagc gcctacgagt tcggcgaggg cgcggccacc gcctacggcg
     4561 tgggcaactc cgtcctggcc ggcaacagcg gcgacgccgt ggtgctcgag aacgtgcagc
     4621 tcaacaccgg cggcggggtc gaggccatcg ccagcttcgc cggcgcggac ggctatgacg
     4681 gctacgcccg ggccaccgcc gtgggcaacg acgccaccgg ctacgcctgc tccgcctgca
     4741 acggctcgat ggccgtcgac agccgccaga ccaacagcgc cgacgtcggc gccacggcga
     4801 ccaccaccat cggatcgggc cgggtgatca actccacctc caccgccgtc ggcaacaacg
     4861 ccagcttcta tgtgacccgg ccgggatcct aggagtcttc gccgcctcac gcgtggactt
     4921 cgaattcggt ggcgttgggc gaccgtccac gaactggagc cgccccggcc gcggcgcgtc
     4981 tattgtcggc aattcgggaa cagtccgaac tcgcgcaccg cggcgccacg ccttcgcctg
     5041 ccccatttac ctctccggat atattggcat tatggatgcc aacttattcg aggccggtcg
     5101 cggcccggga ggattcgcat gctcagcccc tggccgtggg tcggcgcgct ggtcgtctat
     5161 ctgctgttcc gcgcctggta cgacaactgg cgcgggccgc tgacggccgc cgaggtcgcg
     5221 gccttcatgg ccagggtcga ggccggcccg ggcgcggcgc acaatgaccc ggccatcgtg
     5281 cgcgccttcc tcgaggccga cgacggccgc gagttcttca tgttcaacct ggtcaagatc
     5341 gccaccggcg aggcgccgca tcccgaaacc ggcgcgccga cgccgcgcgc gcggttgatg
     5401 cggcgctata cccgcacctt catccccgcc ctgctggccc gtggcggcca cccggcgctg
     5461 gccgcgcgca agatcgccgg ctatgtcgac gcctggaagg tcgggcccga ccccggctgg
     5521 tcgatcatcg gctacatgcg ctaccgcagc cggcgcgaca tgatcgccat ggccacccac
     5581 ccgaaattcc agggcatcca cgacttcaag atcgcaggca cggccgagac cttctcgttc
     5641 ccgacccggc cgatgctgat gctgctggcg ccgccgcggc tgtgggtggc gctggtgctg
     5701 ttgctggcgg ccgccttcgc ccagatcgcc ctgctcgtcc cctgacccgg agattcccga
     5761 tggaccctgt cgccctcagc ggcgcgcccg gctcgcccta cagccgcaag atgctggccg
     5821 tgctgcgcta ccggcgcatt ccctaccggc tgttctggcc cggccgcgag ccggcggact
     5881 atccctcgcc gaaggtgcgg ctgctgccga ccttctacct gccgggcgag gacggcgcgc
     5941 tgacggcggt gaccgactcg acgccgctga tccgccgctt cgagcgggac tacagcgatc
     6001 gactgctgcg cccggccagc ccggcgctgg ccttcctcga ctcgctgctc gaggactatg
     6061 gcgacgagtg gctgaccaag gcgatgttcc actatcgctg ggcctaccgg gccgacatcg
     6121 accaggcggc cggcgtgctg ccgtcctggc gcggcacgct cgacgacgag atgctggcgg
     6181 cgatgggcaa ggccttctcc gaacggcaga tcggccggct gcgctatgtc ggctcgaacc
     6241 cgaccaccgg gccggtgatc gaggccagct acgtgcggct gctcgacctg ctcgaggacc
     6301 atctgaaaca acacggcttc ctgctcggcg gccggcccgg ggcgggcgac ttcggtctct
     6361 acggccagct gacccagctg gcggcgttcg acccgacgcc gatggccctg gcggcgagcc
     6421 gcgctccgcg ggtgatggcc tgggtgcagc tggtcgagga cctgtgcggc ctggaaccgg
     6481 ccgacagcga ctggttcgac ccggccgacc tgcccgacac gctgaaggcc atcctggccg
     6541 agaccggccg cgtctacgcc ccgctgatgg cggccaacgc ccgggccgtg atggccggcg
     6601 ccgagaccgt cgagaccgag atcgacggcc atccctggag ccagaacacc ttcccctacc
     6661 aggccaagtg cctggcctgg ctgcgcgagg aatacgccgc cctgcccgcg ggcgaccggg
     6721 cgctggtcga cgcggccctt gccgggacgg ggtgggcggg gatgtttggg tagggcctta
     6781 ggggccaggg gccaggggcc agggggaatt ctccgtggcg accgacgtga cgagtcccta
     6841 gtccctagcc cctccctgcc cccgtcccgc cttgccttcg ccgcatcgcc cggcgaaggt
     6901 gcgctcaaat ccctccagcc ctgattgagc gtatccccgc atgcatcctc tcgtccgccc
     6961 ggccctcgtc gccgtgacgg ccctgtccct ggttggctgc gccggcgcgg cgggcaggct
     7021 g
//
LOCUS       DAORNX010000112         6985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8024_length_6985_cov_6.676190, whole genome shotgun sequence.
ACCESSION   DAORNX010000112 DAORNX010000000
VERSION     DAORNX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_6985_cov_6.676190"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLO65_06525"
     CDS             <1..333
                     /locus_tag="PLO65_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRD27937.1"
                     /translation="GRLLAEPTLKVFATLPCLAAWGPTAAVAVAQVEVGPTGADQTLW
                     VTDAPDIGRTIEALSRDGVAGGPIAQHGGLTLYALAGYYQPDDARLARAPGRLHGVIG
                     AAPEPFDL"
     gene            379..1509
                     /gene="hisC"
                     /locus_tag="PLO65_06530"
     CDS             379..1509
                     /gene="hisC"
                     /locus_tag="PLO65_06530"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRD27938.1"
                     /translation="MTAMPTDRFKSDRPVPKPGILDIIAYKPGKATAEGIAHPIKLSA
                     NENILGASPMAQAAYAEAAGKLNLYPDPRTTILREGIAARYGLEPERLLFGCGSDEVF
                     QLLCQTFLEPGDNMVQPEHGFAAWAIGARACGAEIRNAPEKDLHVDVDALLAQVDERT
                     RLVFIANPANPTGTWIPFSEIRRLHEALPPSVVLVLDGAYAEFQGGDGGREDFELARH
                     ENNIAVTRTFSKIYGLAALRVGWGYMPAEMTAAIDRIRLPFNVNIPAQLAAVAALGDE
                     DFKQRSIDLVETWRPWLTQQLSGLGLDVAPSAANFVLVGFPRTPGRTAAEAEAFLASK
                     GYLTRGVGNYGLPDHLRITIGLEAHNRAVVEALAEFMGRAGA"
     gene            1624..2556
                     /locus_tag="PLO65_06535"
     CDS             1624..2556
                     /locus_tag="PLO65_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HRD27939.1"
                     /translation="MPGLLFDQLTIVGCGLIGSSIARVAREKGVAGSIVVADPSDPVR
                     ERVAALGLADRVTGDIGDGVTGADLVIFATPVLAIGEAMAAAAGHLKPGAIVTDVGSV
                     KGVVAEALAAHAPAGVFVIPGHPIAGTEHSGPDAGFAELFDGRWTILTPLASDEPGYA
                     EAVAKLSAFWRACGAQVEVMDAAHHDLVLAITSHLPHLIAYNIVGTASDLEGVTEGEV
                     MKYSAGGFRDFTRIAASDPTMWRDIFVANKDAVLEVLGRFTEDLQALSRAIRWGEADK
                     LHDLFTRTRAIRRGVIALGQESPEPNFGRDRGKH"
     gene            complement(2553..3104)
                     /locus_tag="PLO65_06540"
     CDS             complement(2553..3104)
                     /locus_tag="PLO65_06540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD27940.1"
                     /translation="MMRQLLCAAVLALAAASTALAAPSGDQPWVSVTLDPDGESALIN
                     ASIDIPAPARKVWSAMTDCDATRAMIRSLVQCRVVRTGEGWDVREHITRGGPLWPSFR
                     YVFRSDYEAGRRIRFRKLDGNLKTFEGEWDLAPLSGGQATRVTYRTRMAAQIFAPPML
                     VRAGLRKDTPRVLEALRRVATGG"
     gene            3531..4748
                     /locus_tag="PLO65_06545"
     CDS             3531..4748
                     /locus_tag="PLO65_06545"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRD27941.1"
                     /translation="MSGFQYIESTGAPIKAWTQGVPIEESAMTQLRNVASLPFIHKHV
                     AVMPDVHWGMGATVGSVIPTVGAIIPAAVGVDIGCGMMAVRTSIRAEHLPDSLSALRS
                     AIEAAVPHGRTDNGGRNDIGAWQKEPPAVAAAKWAALEDGYKAVVDRHPKAAHPRGFG
                     HLGTLGTGNHFVELCLDEAGALWVMLHSGSRGVGNRFGTYFIERAKHDMRRWFINLPD
                     EDLAYFPEGSEGFIDYIRAVSWAQKYALANREVMMDQVLGVLRETFPDLATDEMAVNC
                     HHNYVSREKHFGKDVFVTRKGAVKAGLGDLGIIPGSMGARSFIVRGKGNPDSFCTCSH
                     GAGRAMSRAEAKRRFTVDDHVAATAGVECRKDAGVIDETPMAYKDIDAVMAAQADLVE
                     IVHTLRQVVCVKG"
     gene            4854..5669
                     /locus_tag="PLO65_06550"
     CDS             4854..5669
                     /locus_tag="PLO65_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD27942.1"
                     /translation="MTTSHPLDQATTLVPTGERRATGATHPAFAIMAGPFGGATAATL
                     LRAVLEDPACIGEPIALTVNFTGAIADGEFEVSWIEKRTGRTVQHWTVDLVQNGKVAA
                     TASVVCGRRTEAWTHRPATMPVAPPAADVAPLDMGTRLGWIQRYAFRFVEGAPGFGEP
                     PADGPRSAKTTCWLSDLPERTLDFLSLAALSDAFIVRIIQVRGKPGPMGTITLTTYFH
                     AGAEALAANGARPILGVADASVFHGGFADQQASLWRDDGVLLATSVQQSFYRD"
     gene            complement(5961..6209)
                     /locus_tag="PLO65_06555"
     CDS             complement(5961..6209)
                     /locus_tag="PLO65_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27943.1"
                     /translation="MTDLPGEPRSFLPGRPPASTLARQVRWHVGLSQQAFALTFRIDP
                     ERLGWIERGEVEPDPVLEAYLRVIDRDPGLVLAALSQR"
     gene            complement(6662..>6985)
                     /locus_tag="PLO65_06560"
     CDS             complement(6662..>6985)
                     /locus_tag="PLO65_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRD27944.1"
                     /translation="VRDGIDVPGRSLRLLVFDRVPWPRPDILHKARRARFGGKGYDDG
                     LARARIAQAFGRLIRRADDRGVFVMLDAAAPTRLFSSLPEGVELQRMGLVEAIEGVAA
                     FLGTA"
BASE COUNT         1103 a         2461 c         2437 g          984 t
ORIGIN      
        1 ggccggttgc tggccgagcc gacgctgaag gtgttcgcca ccctgccctg cctggccgcc
       61 tggggcccga ccgccgccgt ggccgtggcc caggtcgagg tcggcccgac cggcgccgac
      121 cagaccctgt gggtcaccga cgcccccgac atcggcagga ccatcgaggc cctgagccgc
      181 gacggcgtcg ccggcggtcc gatcgcccag cacggcggcc tgaccctcta tgcgctggcg
      241 ggctattacc agcccgacga cgccaggctg gcccgcgcgc cggggcggct gcatggcgtc
      301 atcggcgcgg cccccgaacc cttcgacctt tagccggtcc tcctgtaaga ggccggtccc
      361 tgaactggac ctccgccgat gaccgcgatg ccgaccgacc gtttcaaatc cgaccgcccc
      421 gttcccaagc cgggcatcct cgacatcatc gcctacaagc cgggcaaggc gacggccgag
      481 gggatcgccc atccgatcaa gctgtcggcc aacgagaaca tcctcggcgc ctcgccgatg
      541 gcccaggcgg cctacgccga ggcggccggc aagctgaacc tgtatcccga tccccgcacc
      601 accatcctgc gcgagggcat cgcggccagg tacggcctgg aacccgaacg gctgctgttc
      661 ggctgcggct ccgacgaggt gttccagctg ctctgccaga ccttcctcga gccgggcgac
      721 aacatggtcc agcccgagca cggcttcgcc gcctgggcaa tcggcgcgcg cgcctgcggg
      781 gccgagatcc gcaacgcccc ggagaaggac ctgcacgtcg acgtcgacgc cctgctggcc
      841 caggtcgacg agcgcacgcg gctggtgttc atcgccaacc cggccaaccc gaccggcacc
      901 tggatcccgt tcagcgagat ccgccgcctg cacgaggccc tgccgccctc ggtggttctg
      961 gtgctcgacg gcgcctacgc cgagttccag ggcggagacg gcggccgcga ggacttcgag
     1021 ctggcccgcc acgaaaacaa catcgccgtc acccggacct tctcgaagat ctacggcctg
     1081 gccgcgctgc gcgtgggctg gggctacatg ccggccgaga tgaccgccgc catcgaccgc
     1141 atccggctgc cgttcaacgt caatattccg gcccagctgg ccgcggtcgc ggcgctgggc
     1201 gacgaggact tcaagcagcg gtcgatcgac ctggtcgaga cctggcgtcc gtggctgacg
     1261 caacagctgt cgggccttgg cctggacgtc gccccctcgg cggccaactt cgtgctggtc
     1321 ggctttccgc ggacgccggg caggaccgcg gccgaggccg aggccttcct ggcctcgaag
     1381 ggctatctga cgcgcggcgt gggcaactac ggcctgcccg accacctgcg catcaccatc
     1441 ggcctggaag cgcacaaccg ggccgtggtc gaggccctcg ccgagttcat ggggagggcc
     1501 ggggcgtgat ggcgtggttc gacacgcgct ccgcgctgct caccatgacg tgcagagatg
     1561 ccgtccccgc tcgacgtcat cctgagcagc ggccgcaggc cgcgtgtcga aggacgcact
     1621 gagatgcccg gcctcctgtt cgaccagctg accatcgtcg gctgcggcct gatcgggtca
     1681 tcgatcgccc gcgtcgcgcg cgagaagggc gtggccgggt ccatcgtcgt cgccgacccg
     1741 tccgatcccg tgcgcgagcg cgtcgcggcg ctgggcctgg ccgaccgggt gaccggcgac
     1801 atcggcgacg gggtaaccgg cgcggacctg gtgatcttcg ccacccccgt gctggccata
     1861 ggcgaggcca tggccgccgc ggccggtcat ctgaagcccg gcgccatcgt caccgacgtc
     1921 ggttcggtca aaggcgtggt ggccgaggcc ctggcggccc acgcgccggc cggcgtgttc
     1981 gtcatccccg gccatccgat cgccggcacc gagcactcgg gacctgacgc cggcttcgcc
     2041 gagctgttcg acggccgctg gaccatcctg acgccgctgg ccagcgacga acccggctac
     2101 gccgaggcgg tggcgaagct gtcggccttc tggcgcgcct gcggcgccca ggtcgaggtg
     2161 atggacgcgg cccaccacga cctggtgctg gccatcacca gccacctgcc gcacctgatc
     2221 gcctacaaca tcgtcggcac ggcctcggac ctcgagggcg tcaccgaggg cgaggtgatg
     2281 aagtactcgg ccggcggctt ccgcgacttc acccgcatcg ccgccagcga cccgaccatg
     2341 tggcgcgaca tcttcgtggc caacaaggac gcggtgctgg aggtgctggg ccggttcacc
     2401 gaggacctgc aggcgctgag ccgggcgatc cgctggggcg aggccgacaa gctgcacgac
     2461 ctgttcaccc gcacccgggc catccgccgc ggcgtcatcg cgctgggcca ggagagcccc
     2521 gagccgaact tcggccggga tcgcgggaag cactaacccc ccgtcgccac ccggcgcagg
     2581 gcctcgagca cgcgcggtgt gtccttgcgc aggccggcgc ggaccagcat cggcggggcg
     2641 aagatctgcg cggccatgcg ggtgcggtag gtcacgcgcg tggcctggcc gccgctcagg
     2701 ggggccaggt cccactcgcc ctcgaaggtc ttcaggttac cgtccagctt gcggaagcgg
     2761 atgcggcggc ccgcctcgta gtctgaacga aacacatagc ggaagctcgg ccacagcggc
     2821 ccgccgcgcg tgatgtgctc gcgcacgtcc cagccctcgc cggtccggac cacgcggcac
     2881 tggaccaggc tgcggatcat cgcccgggtg gcgtcgcagt cggtcatggc gctccagacc
     2941 ttccgcgccg gagcggggat gtcgatggac gcattgatca acgccgactc gccgtccgga
     3001 tcgagcgtca ccgagaccca cggctggtcc ccggacggcg cggccagggc ggtcgacgcg
     3061 gccgcgaggg ccagaacggc ggcgcagagg agctggcgca tcatcgggtc tcccgctcgc
     3121 gccgcgaacg gcgcgtcgga cggcgggact agcgtgagtt tgcgacagct gcgcgactcc
     3181 ggcgcaccgt caacgcctgc ggctttcccg ccacaggcga ggggattgtc aaatacagcc
     3241 ttggcgcggc tcaaagaccc atgctatccg cctgccctct tgaggaagag gcccatggct
     3301 cactcgtcgc caatgatttc gcccgccagc aaactgctcg accgccaggt cgagggtctg
     3361 cgtgcatgtg cgtcggaggg acgggtgatc tgaggcttca agccctcaag aattgtccga
     3421 aggaacccct cctggcccgg ccggaggggt tctttcgttt caggaggacg ctttcgacca
     3481 tgacgccagg acgggctgtg cagaaaacgg tttgaaggaa ggagaacccc atgagcgggt
     3541 tccagtacat cgaaagcacg ggcgcgccga tcaaggcgtg gacccagggc gtgccgatcg
     3601 aagagtcggc catgacgcag ctgcgcaatg tcgccagcct gccgttcatc cacaagcacg
     3661 tggccgtcat gcccgacgtg cactggggca tgggcgcgac cgtcggttcg gtgatcccga
     3721 ccgtcggcgc gatcatcccg gcggcggtgg gcgtcgacat cggctgcggc atgatggcgg
     3781 tgcggacgtc aatccgcgcc gagcatctgc cggactcgct gtcggccctg cggtcggcga
     3841 tcgaagccgc ggtgccgcac ggccggaccg acaacggcgg ccgcaacgac atcggcgcct
     3901 ggcagaagga gcccccggcg gtggcggcgg ccaagtgggc cgcgctcgaa gacggctaca
     3961 aggccgtcgt cgaccggcac ccgaaggccg cgcatccgcg cggattcggc cacctgggta
     4021 cgcttggcac cggcaaccac ttcgtcgagc tctgcctcga cgaagcgggc gcgctgtggg
     4081 tcatgctgca ctccggttcg cggggcgtag gcaaccggtt cgggacctat ttcatcgaac
     4141 gggccaagca cgacatgcgg cggtggttca tcaacctgcc ggacgaagac ctcgcctatt
     4201 tcccggaagg gagcgagggc ttcatcgact acatccgggc ggtcagctgg gcgcagaagt
     4261 acgcgctggc gaaccgggaa gtgatgatgg atcaggtgct cggggtgttg cgggaaacct
     4321 tcccggacct cgcgacggac gaaatggcgg tgaactgcca ccacaactac gtcagccgcg
     4381 agaagcactt cggcaaggat gtgttcgtga cccggaaggg cgcggtgaag gcgggtctcg
     4441 gcgatctggg gatcatcccc ggatcgatgg gcgcccggtc gttcatcgtg cgcggcaagg
     4501 gcaacccgga ctcgttctgc acctgctcgc acggcgccgg ccgggccatg agccgggccg
     4561 aagccaagcg ccggttcacg gtcgacgacc atgtcgcggc cacggccggg gtcgagtgcc
     4621 gcaaggacgc gggcgtcatc gacgaaacgc cgatggccta caaggacatc gacgccgtca
     4681 tggccgccca ggccgacctg gtggagatcg tccacaccct gcgtcaggtg gtgtgcgtga
     4741 agggctgagg cggggacccg tcttccgaaa gggaggcggg tccttgccac catgcgggtc
     4801 ctttttgaaa actcatcttg cctgacgtgg cgtcgctggt aggaaatggc tccatgacca
     4861 cctcgcaccc cctcgatcag gccacgaccc ttgtcccgac cggcgagcgg cgggcgaccg
     4921 gcgcaaccca cccggccttt gccatcatgg ccgggccgtt cggcggagcg acagcggcga
     4981 cgctgctgcg ggcggtgctc gaggatccgg cctgtatcgg cgagccgatc gccctgacgg
     5041 tcaacttcac cggggccatc gccgacggtg agttcgaggt cagctggatc gagaagcgca
     5101 cgggccgcac ggtgcagcac tggaccgtcg acctggtgca gaacggcaag gtggcggcca
     5161 ccgcctcggt ggtctgcggt cgccggacgg aggcctggac ccatcggccg gcgaccatgc
     5221 cggtcgcgcc cccggccgcc gacgttgcgc ccctggacat gggaacgcgg ctgggctgga
     5281 tccagcgcta cgccttccgc ttcgtcgagg gcgcgccggg attcggcgaa ccgcccgccg
     5341 acggcccccg cagcgcgaag accacctgct ggctcagcga cctgcccgag cggacgctgg
     5401 atttcctgtc cctggccgcc ctcagcgacg ccttcatcgt gcgcatcatc caggtgcgcg
     5461 gaaagccggg gccgatgggc acgatcaccc tgacgaccta tttccacgcc ggcgcggagg
     5521 ccctggcggc gaatggcgcc cggccgatcc tcggcgtggc cgacgccagc gtgttccacg
     5581 gcggcttcgc cgaccagcag gccagcctgt ggcgcgacga cggcgtcctg ctggcgacca
     5641 gcgtgcagca gtcgttctat cgggactagg gccaatcgac attcatggcc cggggccgtc
     5701 ttttcaccga ttgtcccgcg aaggcgggga cccaagcccg tatggccgcc gaaacggcgc
     5761 gacaggaccc gaaacaccgc ttgggttccc gccttcgcgg gaactatcgg agccctcggc
     5821 ggctcccgcg cgctgaatgt cgattggacc tagacgggga ccggatgccc tgtccccccg
     5881 cgcgggagat cggggacagc tcatgacgtc ccgagggacg ccataagcag tccccgcgat
     5941 gaggggatca cccccgccga tcaacgctgc gacagcgccg ccagcacaag gcccgggtcg
     6001 cggtcgatga cgcgcagata ggcctccagc accggatcgg gctcgacctc gccgcgctcg
     6061 atccagccga gccgttcggg atcgatgcga aaggtcaggg cgaaggcctg ctgcgacagc
     6121 ccgacatgcc agcggacctg tcgcgcgagg gtggaggcgg gcgggcgacc gggcaggaag
     6181 gaccggggtt ccccgggaag atccgtcata tcagtcgcct ccgtcacgct actcaagccc
     6241 cgtgacaacg ggagtagcgc agaggcgttg caggaataag acggcgggga gtcaggacgg
     6301 gggtgacttc tgggtcatcg accgccgccc cctctcattc tcgcatccgc atggatgaag
     6361 gggaagacaa cgacctgatg acacctgccc caaaggcctt gatggaatgg gagcgcgagc
     6421 ggtcgtcttg gtcaaggacg gccctccggg ccgccgctgc gcggccggcg cagccggtcc
     6481 ttgagcaagc cgcccgtccg cgcaatgctc cgccaaggct gaagggcggg gagtgcggga
     6541 gctcggaagc ggattggctg aaggtccgct gagggtgggg agcagaagtt agcgccgcgc
     6601 cgcggagcgc cccctcgatg aagaccttgg ggcggatgtt ggcccggctc cctggtgagc
     6661 cctacgccgt ccccagaaac gccgccaccc cctcaatggc ctcgaccagc cccatccgct
     6721 gcaactccac cccctcgggc aggctgctga acaggcgggt cggggcggcg gcgtcgagca
     6781 tcacgaacac gcccctgtcg tcggcccggc ggatcaagcg gccgaaggcc tgggcgatgc
     6841 gggcgcgggc caggccgtcg tcataaccct tgccgccgaa gcgcgcgcgg cgggccttgt
     6901 gcaggatgtc cgggcgcggc cagggcacgc ggtcgaacac cagcaggcgc agcgagcggc
     6961 cgggcacgtc gatgccgtcg cgcac
//
LOCUS       DAORNX010000113         6956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8060_length_6956_cov_7.243733, whole genome shotgun sequence.
ACCESSION   DAORNX010000113 DAORNX010000000
VERSION     DAORNX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_6956_cov_7.243733"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>245
                     /locus_tag="PLO65_06565"
     CDS             <1..>245
                     /locus_tag="PLO65_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD27945.1"
                     /translation="SRLGAAVRLIGRVGADGMADEALALLRADGVDLSGVAVDPAAAT
                     GVALIGVDAEGENQIIVAPGANAAFTADLLPATLDGA"
     assembly_gap    246..399
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <400..713
                     /locus_tag="PLO65_06570"
     CDS             <400..713
                     /locus_tag="PLO65_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD27946.1"
                     /translation="VYGKRLHGLPGLVAVTWGSRGAGLFRDGARLATAEPPDVVAVDA
                     TGAGDAFVGALTVALLEGMGRAEALAFACAAGALAATRPGAQSSLPARAEVERVLQAR
                     H"
     gene            782..1705
                     /locus_tag="PLO65_06575"
     CDS             782..1705
                     /locus_tag="PLO65_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRD27947.1"
                     /translation="MTRSVPELSLRAFTEGDADQRRAFSDAMMRGLQDYGFFVLKDHG
                     VPLELLQRAYGEAEALFALPEATKRRYASGMRGYTPFGVEHAKDSGHPDLKEFWQVGH
                     DPSPESGADLEPNVWPAEAPGVRPAFQSLFSALNETGIVLLRALAPKLGLAVDYFDDK
                     VRYGTSLLRVLHYPPLAPDADPNAVRSAAHEDINFLTIMVAARGAGLELLDRDGTWLP
                     VVTEPTNLIVDSGDMLARMTNGVIPATTHRVVNPTGPNVSRYSMPFFMHPTLETSLEV
                     LPSCLGDGARFEPVTAGAFLDERLRAIGLKA"
     gene            complement(1719..3290)
                     /locus_tag="PLO65_06580"
     CDS             complement(1719..3290)
                     /locus_tag="PLO65_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD27948.1"
                     /translation="MTTPTPINPFAGMDARNLIDLQAASRGDHPCLIWEPFDGEGFTW
                     SYAGLAIAIRRFAAGLQARGVKPADRVLVHLDNCPESVIAWLGCAYAGAVAVTTNARS
                     SADEISYFASHSGAVGGITQPRFAALVREAAPTLGWLVVTEHDNGAPAEADTTGFEPF
                     AALDGDPATLADRPHDPMAHFAIQYTSGTTARPKAVLWTHANFLWGAKMCALHEDLRP
                     TDVHLVTLPLFHTNAQSYSVAASLWAGATMVLQPKFSASRFWPVSQKHGCTWASMVPF
                     CLKAIAEQPRPERHSYRFWGNGVCEPPTDALFGVKTIGWWGMTETITQGTVGSPHLKD
                     APMSMGRPSPGYRIHVLDEAGAPVKPGETGDLLVEGVRGVSLFLEYASDPEATAKAYT
                     VDGLFITGDRVRLGEDGFLYFADRSKDMLKVGGENVAASEIERVVASVAGVRECAVVG
                     RRHPMLDEVPVAFVIPVDAADATLPGRITEACAATLAGFKRPHEVRLVDSLPRSTLEK
                     IAKAELRAMLASERD"
     gene            3396..4046
                     /locus_tag="PLO65_06585"
     CDS             3396..4046
                     /locus_tag="PLO65_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD27949.1"
                     /translation="MIAQAEDSRTDRRRARTRAALLQAGQALFAAQSVDAVSIDEIVL
                     AADVAKGSFYNHFPDKEALAREIAVQVRAEVEAQVDLANRTVTDPALRMARAQAVVIR
                     FALRESEPARAMTRLFAGATLPDAPMNRGVRADIEAGLKAGCFHGLTVEAGVLLAMGA
                     ATAAVSGVLEPAGRLAADRLAGELIFGLLRGLGVADPVARDTSAAAVSDIFAEFQS"
     gene            4043..4969
                     /locus_tag="PLO65_06590"
     CDS             4043..4969
                     /locus_tag="PLO65_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27950.1"
                     /translation="MSLIKIEDIAHVRFRAPDLDEMQRYLEQFGLTLADRSETRLFMR
                     GRGGAPFLHATERGEPGFAAIGLRAESLADLEALAKAEGAPVEALDAPGGGKVVRLTD
                     PDGHIVEVVAGQAFSTPETFPPRLPWNGADGHARLRTVKRTGQGPANVFRLGHAVLGV
                     SNFRASEAWYKARFGFITSDEIALSPDFSIGAFLRCDRGDTPTDHHTLFLLQDPKGPG
                     FNHAAFEVRDLDDLMAGHDHLKAASRTPAWGVGRHILGSQVFDYWADPWGHVLEHWTD
                     GDLLTAADGSNVSSMQELLGMQWGPPPGPAFG"
     gene            4981..5826
                     /locus_tag="PLO65_06595"
     CDS             4981..5826
                     /locus_tag="PLO65_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD27951.1"
                     /translation="MKLATFTAGGAPELGAVVGDRIVPLNRAAPGLPGDMIGLIKAWP
                     AVETEVRRLADSGAGALALDSVRLLAPIPRPGKIWAIGLNYADHIAESGVETPPNQVW
                     FTKAGTAVTGPFDPVQVPTPFCDYEVEMVAVIGAGGRNIGKADAPAAVFGWCVGNDVT
                     ERMWQHRTPQWSLGKSFDTHAPFGPWITTADEIADPHALDLSCAINGETRQNSNTKHL
                     VFNVWDQIEHLSTAMTLEPGDVIFTGTPGGVGAAMDPRRFLKAGDVMRCEVEGLGAIE
                     AICEA"
     gene            5826..>6956
                     /locus_tag="PLO65_06600"
     CDS             5826..>6956
                     /locus_tag="PLO65_06600"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HRD27952.1"
                     /translation="MNRPYDCDVLIVGLGPVGAALAALLARYGVTTIAVERDTEVYPL
                     PRAAHFDHEIMRLFQQLGISGEIEAAARVAPTYEFRNAAGEVLMSYDLTGVVSPSGWA
                     ASYMFHQPSVETALRRLLAESPLVDARLQRTFREFVEADDGVNATIDGPDGTETVRAR
                     WLVGCDGAKGPVRDAIGVGLSDLNFDEPWLVIDALVSDVTRLPQTNLQICDPERPTTC
                     LQMSQGRHRWEFMLLPGEQPEDVLDDAVIRRLLAPFDCDDVVQIERKAVYRFHGRIAE
                     QWRKGRVLLAGDAAHQTPPFAGQGMCSGLRDAANLAWKLAAVIKSGADESLLDTYQTE
                     REPQVRGVIELAIAMGRVVCITDPAAAAQRDAGMLAQRAAGGA"
BASE COUNT          998 a         2397 c         2386 g         1021 t
ORIGIN      
        1 cagccggctg ggcgcggcgg tgcggctgat cggccgcgtg ggcgccgacg gcatggccga
       61 cgaagccctg gccctgctgc gggccgatgg cgtggacctg tcgggagtcg cggtcgatcc
      121 ggcggcggcc acgggcgtgg ccctgatcgg ggtcgacgcc gagggcgaga accagatcat
      181 cgtcgccccg ggggccaatg cggccttcac ggcggacctg ttgccggcca ccctcgacgg
      241 cgcccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggtctatggc aagcggctgc
      421 acggtctgcc tggcctggtg gcggtcacct ggggctcgcg cggagcgggc ctgttccgcg
      481 atggcgcccg gctggccacg gccgagccgc cggacgtggt cgcggttgac gcaacggggg
      541 cgggggatgc ctttgtcggc gcgttgaccg tcgccctgct cgagggcatg ggccgggccg
      601 aggccctcgc cttcgcctgc gccgccgggg ccctggccgc cacgcggcct ggcgcgcagt
      661 cgtccctgcc ggcacgggcg gaggtggaaa gggtgttaca ggctcgccac tagccgctca
      721 tcccggcatt cgccgggatg agcgggtttt agagttgaac gcgagggtct agcttgcgcc
      781 catgacccga tccgttcccg aactcagcct cagggccttc accgagggcg acgctgacca
      841 gcgccgggcc ttttccgacg cgatgatgcg cggtctgcag gactacggct tcttcgtgct
      901 gaaggaccat ggcgtgccgc tcgaactgct gcagcgggcc tatggcgagg ccgaggcgct
      961 gttcgccctg cccgaggcga ccaagcgccg gtatgccagc ggcatgcggg gctacacgcc
     1021 gttcggggtc gagcatgcga aggacagcgg ccatccggac ctcaaggagt tctggcaggt
     1081 cggtcacgat ccctcgccgg aatcgggcgc cgaccttgag cccaatgtct ggccggccga
     1141 ggcgccgggc gtccggccgg cgttccagag cctgttcagc gcgctcaacg agaccggcat
     1201 cgtgctgctg cgcgcgctgg cgccgaagct tggcctggcc gtcgactatt ttgacgacaa
     1261 ggtgcgctac ggcacctcgc tgctgcgcgt gctgcactac ccgccgctgg cgccggacgc
     1321 cgatccgaac gccgtgcgct ccgcggcgca cgaggacatc aactttctga ccatcatggt
     1381 cgccgcccgg ggcgcggggc tcgaactgct cgaccgcgac ggaacctggc tgccggtggt
     1441 caccgagccg accaatctga tcgtcgacag cggcgacatg ctggcccgca tgaccaacgg
     1501 cgtgattccg gcgacgaccc atcgggtggt caatccgacc ggtccgaacg tgagccgcta
     1561 ttcgatgccg ttcttcatgc atccgacgct ggagacctcg ctggaggtcc tgccgagttg
     1621 cctcggcgat ggcgcgaggt tcgagccggt gacggccggc gccttcctcg acgagcggct
     1681 gcgggcgatc gggctgaaag cctaggcgat cacggccgtc agtctctttc ggacgccagc
     1741 atcgccctga gctccgcctt ggcgatcttc tcgagggtcg agcgcggcag gctgtcgacc
     1801 agccgcacct cgtggggccg tttgaagccg gcgagcgtcg cggcgcaggc ctcggtgatc
     1861 cggccgggca gggtggcgtc ggccgcgtcg accggaatca cgaacgccac ggggacttcg
     1921 tcgagcatcg ggtggcggcg gccgaccacg gcgcactcgc gcaccccggc caccgaggcc
     1981 accacccgct cgatctccga cgccgcgaca ttctcgcccc cgaccttgag catgtccttg
     2041 ctgcggtcgg cgaaatacag gaagccgtcc tcgcccagcc gcacccggtc gccggtgatg
     2101 aacaggccgt cgaccgtgta ggccttcgcg gtcgcctcgg gatcgctggc gtactcgagg
     2161 aacagcgaca cgccgcgcac cccctcgacc agcaggtcgc cggtctcgcc cggcttcacc
     2221 ggggcgccgg cctcgtcgag cacatggatg cggtagccgg gcgagggtct gcccatggac
     2281 atcggcgcat ccttcaggtg cggactgccg acggttccct gggtgatggt ctcggtcatg
     2341 ccccaccagc cgatggtctt gacgccgaac agggcgtcgg tcggcggctc gcagacgccg
     2401 ttgccccaga agcggtagct gtgccgctcg ggtcggggct gctcggcgat ggccttgagg
     2461 cagaagggca ccatcgaggc ccaggtgcag ccgtgcttct gcgacaccgg ccagaaccgc
     2521 gaggccgaga acttcggctg cagcaccatg gtcgccccgg cccacagcga ggccgcgacc
     2581 gagtaggact gcgcgttggt gtggaacagc ggcagggtca ccaggtggac gtcggtcggc
     2641 cgcaggtcct cgtgcagcgc gcacatcttc gcgccccaca ggaagttggc gtgtgtccac
     2701 agcacggcct tgggccgggc cgtggtgccc gaggtgtact ggatcgcgaa atgggccatc
     2761 ggatcgtgcg gccggtcggc gagggttgcc gggtcgccgt ccagcgccgc gaatggctcg
     2821 aagcccgtcg tgtcggcctc ggcgggggcg ccattgtcgt gctcggtgac gaccagccag
     2881 ccgagggtcg gggcggcctc gcgcaccagg gccgcgaacc gcggctgggt gatcccgccc
     2941 accgcgccgc tgtggctcgc gaagtagctg atctcatcgg cgctggacct ggcgttggtg
     3001 gtcacggcga cggccccggc ataggcgcag ccgagccagg cgatgaccga ctccggacag
     3061 ttgtcgagat gcaccagcac ccggtcggcg ggcttcacgc ctcgcgcctg caggcccgcc
     3121 gcgaaccggc gaatcgcgat ggcgagcccg gcgtaggacc aggtgaagcc ctcaccgtcg
     3181 aacggctccc agatcaggca ggggtggtcg ccccgcgacg ccgcctgcaa gtcgatcagg
     3241 ttgcgggcgt ccatgccggc gaaggggttg atcggcgtgg gcgttgtcat cagcgtttcc
     3301 ccgagaagcc cctggcgaat gccccatcgg aggccggctt gcaattctga ctgattagtc
     3361 taatagtgac taaatagtca aatatgtgat gcgccatgat cgcccaggct gaagactcca
     3421 ggaccgaccg ccgccgcgcc cggactcgcg ccgccctgct ccaggcgggg caggccctgt
     3481 tcgcggccca gtcggtggac gcggtcagca tcgacgagat cgtgctggcc gctgacgtgg
     3541 ccaagggcag cttctacaac cacttccccg acaaggaggc cctggcccgg gagatcgccg
     3601 tgcaggtccg cgccgaggtc gaggcccagg tcgacctggc caaccggacc gtgaccgatc
     3661 cggccctgcg catggcccgg gcccaggccg tggtgatccg cttcgccctg cgcgaatccg
     3721 agccggcgcg ggccatgacc cgcctgttcg ccggcgcgac cctgccggac gccccgatga
     3781 atcgcggcgt gcgcgccgac atcgaggccg gtctgaaggc aggctgcttt cacggcctga
     3841 cggtcgaggc gggtgtgttg ctggccatgg gcgcggccac ggccgcggtc tctggcgtgc
     3901 tggagccagc gggccggctg gccgccgacc gactggcggg cgagttgatc ttcggcttgc
     3961 tgcgcggcct gggagtcgcc gaccccgtgg cccgggacac cagcgccgcc gccgtttccg
     4021 acatattcgc ggagtttcag tcatgagcct gatcaagatc gaagacatcg cccatgtgcg
     4081 cttccgcgcg cccgacctgg acgaaatgca gcgctatctc gaacagttcg gcctgactct
     4141 cgccgaccgt tcggagaccc gcctgttcat gcgcggccgg ggcggtgcgc ccttcctgca
     4201 cgccaccgaa cggggcgagc cggggttcgc cgcaataggc ttgcgggccg agagcctggc
     4261 cgatcttgaa gccctggcga aggctgaggg cgcgccggtc gaagctctcg acgcgccggg
     4321 cggtggcaag gtcgtgcgcc tgaccgatcc ggacggccac atcgtcgaag tcgtcgcggg
     4381 ccaggctttc agcacgcccg agaccttccc gccccggctg ccctggaacg gagccgacgg
     4441 ccacgcccgc ctgcgcacgg tcaagcgcac cggccagggc ccggccaacg tcttccgcct
     4501 cggccatgcg gtgctggggg tctccaactt ccgggcgtcg gaagcctggt acaaggcgcg
     4561 cttcggcttc atcacctccg atgagatcgc cctgtcgccg gacttctcga tcggcgcctt
     4621 cctgcgctgt gaccgcggcg acaccccgac cgaccaccac accctgttcc tgctgcagga
     4681 tcccaagggg cccggcttca accacgcggc cttcgaagtg cgcgacctgg acgacctgat
     4741 ggccggccac gaccatctga aggccgccag tcgcaccccg gcctggggcg tgggccgcca
     4801 tatcctcggc agccaggtgt tcgactactg ggccgatccc tggggccatg ttctggaaca
     4861 ctggactgac ggcgacctgc tgacggccgc cgacggatcc aatgtctcgt cgatgcagga
     4921 actgctcggc atgcaatggg ggccgccgcc cggcccggcc ttcggttagg agacgccgcc
     4981 atgaaactcg ccaccttcac cgccggcggc gcgccggagc tgggcgccgt tgtcggggac
     5041 aggatcgtcc ccctcaaccg cgccgcgccc ggcctgccgg gcgacatgat cggtctgatc
     5101 aaggcctggc cagccgtcga gacagaggtc cgtcgcctcg ccgacagcgg cgcgggcgcc
     5161 ctggcgctgg acagcgtgcg cctgctcgcc ccgatcccgc ggcctggcaa gatctgggcg
     5221 attggcctga actacgccga ccatatcgcc gagagcggcg tggagacccc gcccaaccag
     5281 gtctggttca ccaaggcggg caccgccgtg accggaccct ttgacccggt gcaggttccg
     5341 acccccttct gcgactatga ggtcgagatg gtcgcggtga tcggcgccgg cggccgcaac
     5401 atcggcaagg ccgacgcgcc cgccgccgtg ttcggctggt gtgtcggcaa cgacgtcacc
     5461 gagcggatgt ggcagcaccg cacgccgcag tggtcgctgg gcaagtcgtt cgacacccat
     5521 gcgccgttcg gcccctggat caccaccgcc gatgagatcg ccgacccgca cgccctggac
     5581 ctgagctgcg ccatcaacgg cgagacccgt cagaactcga acacgaagca cctcgtgttc
     5641 aacgtctggg accagatcga acacctctcg acagccatga cgctggagcc gggcgacgtg
     5701 atcttcaccg gcacccccgg cggcgtgggc gcggcgatgg acccgcgccg gttcctcaag
     5761 gccggcgatg tcatgcgctg cgaggtcgag ggcctgggcg ccatcgaagc aatctgcgag
     5821 gcgtgatgaa caggccctac gactgcgacg ttctgatcgt cggcctcggg cccgtcggcg
     5881 cggccctcgc ggcccttctg gcccgctacg gcgtcacgac gatcgcggtg gagcgcgaca
     5941 ccgaggtcta tcccctgccc agggccgccc acttcgacca cgagatcatg cggctgttcc
     6001 agcagctggg gatctcgggc gagatcgagg ccgccgcgcg cgtggcgccg acctacgagt
     6061 tccgcaacgc cgccggcgaa gtcctgatga gctacgacct caccggcgtg gtcagcccgt
     6121 cgggctgggc ggccagctac atgttccacc aacccagcgt ggaaacggcc ctgcgccgcc
     6181 tgctggcgga aagtccgctg gtggacgcgc ggctgcagcg caccttccgc gagttcgtcg
     6241 aagccgacga cggcgtgaac gcgaccatcg acgggcccga cggaaccgag acggtccgcg
     6301 cccggtggct ggtcggctgt gacggggcca agggcccggt gcgcgacgcg atcggggtcg
     6361 gcctgtccga tctcaatttc gatgagccct ggctggtgat cgacgcccta gtcagcgacg
     6421 tgacgcggct gccgcagacc aatctgcaga tctgtgatcc ggagcgaccc acgacctgcc
     6481 tgcagatgag ccagggccgc caccgttggg agttcatgct gctgcccggc gagcagcccg
     6541 aagacgtgct cgacgatgcc gtcatccgcc ggctgctggc cccgtttgac tgtgacgacg
     6601 tcgtccagat cgaacgcaag gccgtctacc gctttcacgg cagaatcgcc gaacagtggc
     6661 gcaagggccg ggtgttgctg gcgggggatg ccgcccacca gaccccgccg ttcgccggac
     6721 agggcatgtg ctcgggcctg cgcgacgcgg ccaacctggc gtggaagctg gccgccgtca
     6781 tcaaaagcgg cgccgacgag tcgctgctcg acacctacca gaccgagcgc gagccgcagg
     6841 tccgcggcgt catcgaactg gccatcgcca tgggtcgggt ggtctgcatc accgatcccg
     6901 ccgcggccgc ccagcgggac gccgggatgc tggcccagcg ggccgcgggc ggcgca
//
LOCUS       DAORNX010000114         6932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8090_length_6932_cov_8.000436, whole genome shotgun sequence.
ACCESSION   DAORNX010000114 DAORNX010000000
VERSION     DAORNX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8090_length_6932_cov_8.000436"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1910)
                     /locus_tag="PLO65_06605"
     CDS             complement(123..1910)
                     /locus_tag="PLO65_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD27953.1"
                     /translation="MSDQYEDEEVTRGRVLSNGQVMAFIAGFWLRRRWLLLGSAGGML
                     LAMAFELALPWAARGLVDAVTGAGRTAGGAWIAWSLFVGAYLAFSITRNLAFRFWNPL
                     AARNMEEMTNEGFRRVQAFSADWHADTFAGATVRRLSRAMWGYDSVSDAVVLWLGPAL
                     LLLVGQSVLIALQWPLVGVVALLVVFGFLWINLWVVKVYVRPANLRSNALDSRIGGAL
                     ADALGSNPTVKGFGAEAREEARFAGLTAEWRTAVQVTWGRFMDIWLLQNLLTMALQAV
                     MTGTLVWLWTKGRATPGDIAFGITTFMQMNGYLRNIGENVRMFQKGVDDVEDVANYAR
                     MTPQVADPADPQPFRAEEGEIVFDAVTFRYKSAEAPLYEGFSLRIAPGERVALVGPTG
                     AGKSTFVKLIQRLYDLNAGAIRIDGQDIATVAQGELRRAIAVVPQDPALFHRTIAENI
                     GYARPGATLEEIRLAAKRARADEFIDKLPKGYDTLVGERGVKLSGGERQRVAIARAFL
                     ADAPILVFDEATSSLDVETELSVQAAMEELMAGRTTIVIAHRLSTIRHADRILVFDRG
                     RIVEEGAHAELKARGGAYARLAAVAEGTG"
     gene            complement(2057..2338)
                     /locus_tag="PLO65_06610"
     CDS             complement(2057..2338)
                     /locus_tag="PLO65_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HRD27954.1"
                     /translation="MERPPPNRHRSPAARAALDGLGATLVAVGAVGVVTPGLPTTVFW
                     IGAVVCFLKTRPHAVRPLLRTPVVGPAIIGFLRWRPFGGGRKKGAGRRE"
     gene            2385..3314
                     /gene="ubiA"
                     /locus_tag="PLO65_06615"
     CDS             2385..3314
                     /gene="ubiA"
                     /locus_tag="PLO65_06615"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRD27955.1"
                     /translation="MSSILPDAAPTNWVDRHAPAGLRPWLKLGRFDRPTGIWLLMLPG
                     WMGIALAAAMRGKAPDLVLMLLFFIGAALMRAAGCAYNDIVDRDIDARVARTAGRPIP
                     AGQISVRQAWAFVIGCCLVSLLILLAMNWWTIGLGVASLALVAAYPFMKRITWWPQAW
                     LGLTFNWGALMGFAAVAGMPGWPAVLLYAGLVFWTLGYDTIYAVQDLEDDALAGVKSS
                     ALRLGEQAPRAVLLFYGVCFGLVLTGAWVGGLGPLFLPPAALFALHLSRQASRLDVAD
                     PAGALALFKSNSLAGLVLVVALAAGAWKPGAVF"
     gene            complement(3346..3696)
                     /locus_tag="PLO65_06620"
     CDS             complement(3346..3696)
                     /locus_tag="PLO65_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27956.1"
                     /translation="MRVRLFAAVLAAGILGAGAAGAASFNCARAATPDERAICDDRAL
                     NDQDVRMALLYDIVRRLVPMGSRGAIMDEQSRWLRQRRTCGPDRACLSRSYATRIARL
                     NRVLETRVYPNGPF"
     gene            3764..4726
                     /locus_tag="PLO65_06625"
     CDS             3764..4726
                     /locus_tag="PLO65_06625"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HRD27957.1"
                     /translation="MAVAARVKPKDFFTDEEWAPLAARSSWKGLWLVAHAWLTILAAG
                     ALFVIWPNPLTLLLAVMIIGARQLGLAILMHDGAHGCLHPNQKVNDWVGHWLCGAPTN
                     GDLRRYRDYHLQHHKFAQQQEDPDLGLSAPFPITRQSLWRKAVRDLTGQTFYKQRIKP
                     SLDAFRTGAKKGVPFGKTAKGVLKFWSPFLIFHLPVIAVLSACGLWWAWPVLWLLPMA
                     TWYPLITRLRNIAEHALVGVNQPDPLRHARTTLAGPVERLILAPYYVNYHCEHHMFMH
                     VPCWNLPKVHRLLKQKGVLEKMLTAPGYVAVLREAAGRLEAQPA"
     gene            complement(4714..5475)
                     /locus_tag="PLO65_06630"
     CDS             complement(4714..5475)
                     /locus_tag="PLO65_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HRD27958.1"
                     /translation="MTDGPPRKQATLYRMVMEKHVCPWGLKSRHLLHSKGYVIDDHWL
                     RTREETDAFKAGHKVETTPQTFIDGRRIGGYDDLRRFFGLKVRDPDQKTYTPVLAVFA
                     VAALMTLAASQAAFGTLLTLRAGEWFIAFSMCLLAMLKLRDLASFSTMFLGYDLLARR
                     WVPYGYVYPFAELGAGVLMIAGVLTWLAAPVALFIGVIGAVSVFKAVYIQKRDLKCAC
                     VGGDSNVPLGFVSLTENLMMIAMAVWMLVRMVQAG"
     gene            5575..6225
                     /locus_tag="PLO65_06635"
     CDS             5575..6225
                     /locus_tag="PLO65_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD27959.1"
                     /translation="MITDRRAFIVENTRLQAPPHAPELVLHLADEITPIWKLTEEALA
                     EIGLPPPFWAFAWAGGQALARYVLDNPDIVAGKRVVDFASGSGMVAIAAMKAGAASAL
                     AADIDVFCEAAVALNAAANGVVVDFTDLDLLDAPAPDWAEVILAGDICYEKPLAERVI
                     AWLTAARAAGKTVLIGDPGRTYFPRTGLTLLAEYQVPTTRELEDMEVKRTRVWTFP"
     gene            6275..6625
                     /locus_tag="PLO65_06640"
     CDS             6275..6625
                     /locus_tag="PLO65_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD27960.1"
                     /translation="MREDGKIDYVEFPADGIPAVKDFYSKAFGWTWVDYGPDYAAFGD
                     AAVDGGFNGDSTDAGRAAGPLVVLYAHDIEAMADRVTAAGGVIINPIYSFPGGRRFHF
                     TDPSGNELAVWSET"
BASE COUNT         1077 a         2450 c         2351 g         1054 t
ORIGIN      
        1 tccgggccac ccacgaacac ggtcctggaa acaaggccac ggcgctaccg ccacctggtc
       61 ggacggctca atgtgcctgg gtggcccgga caagccgggc catgacggtt gttttcaggg
      121 gcctaacctg tcccttcggc caccgcagcc agccgcgcat aggccccgcc ccgcgccttc
      181 agctcggcgt gggcgccttc ctcgacgatg cgccccctgt cgaacaccag gatgcggtcg
      241 gcgtggcgga tggtcgacag gcggtgggcg atgacgatcg tcgtgcgccc ggccatcagt
      301 tcctccatgg ccgcctggac ggacagctcc gtctcgacgt cgagcgaact ggtggcctcg
      361 tcgaacacca ggatcggggc gtcggccagg aaggcgcggg cgatggccac ccgctggcgc
      421 tcgccgccgc tgagcttgac gccgcgctcg ccgaccaggg tgtcgtagcc cttgggcagc
      481 ttgtcgatga actcatcggc gcgggcgcgc ttcgccgcga gccggatctc ctcgagcgtc
      541 gcgcccggcc gggcgtagcc gatgttctcg gcgatcgtac ggtggaacag ggccgggtcc
      601 tgcggcacca cggcgatcgc ccggcgcagt tcgccctggg ccacggtcgc gatgtcctgg
      661 ccatcgatgc ggatggcgcc ggcgttgagg tcgtatagcc gctggatcag cttgacgaag
      721 gtcgacttgc ccgcgcccgt cggcccgacc agcgccaccc gctctccggg ggcgatgcgc
      781 agggagaagc cctcgtacag cggcgcctcg gcgctcttgt accggaaggt cacggcgtcg
      841 aagacgatct ccccctcctc ggccctgaag ggttgcggat cggccgggtc ggcgacctgg
      901 ggcgtcatgc gggcatagtt ggccacgtcc tcgacgtcgt cgacgccctt ctggaacatg
      961 cgcacgttct cgccgatgtt ccgcaggtag ccattcatct gcatgaaggt ggtgatgccg
     1021 aaggcgatgt cgcccggcgt cgcgcggccc ttcgtccata gccagaccag ggtccctgtc
     1081 atcaccgcct gcagggccat ggtcagcagg ttctgcagca gccagatgtc catgaaccgg
     1141 ccccaggtga cctgcaccgc cgtgcgccat tcggcggtca ggccggcgaa tcgcgcctcc
     1201 tcgcgggctt cggcgccgaa gcccttgacg gtcggattgg agcccagggc gtcggccagg
     1261 gccccgccga tccgcgaatc cagcgcgttg gagcgcaggt tggccggccg cacatagacc
     1321 ttcacaaccc acaggttgat ccaaagaaag ccgaacacca cgagcagggc cacgacgccg
     1381 accagcggcc actgcagagc gatcaggact gactggccga ccagcagcag aagcgccggt
     1441 cccagccaca ggaccacggc gtcggacacc gaatcatagc cccacatggc ccgcgacagc
     1501 cgccgcacgg tggccccggc gaaggtgtcg gcgtgccagt cggccgagaa ggcctgaacc
     1561 cggcggaagc cctcgttggt catctcctcc atgttccgcg cggccagcgg gttccagaag
     1621 cggaaggcca ggttgcgggt aatggagaag gccagatagg cgccgacgaa caggctccag
     1681 gcgatccagg cgccaccggc ggtgcggccc gcgccggtca ccgcgtcgac caggccgcgc
     1741 gccgcccagg gcagggccag ctcgaaggcc atggccagca gcatcccgcc cgcggagccg
     1801 agcagcagcc atcgccgccg cagccagaag ccggcgatga aggccatgac ctggccgttg
     1861 gagagcaccc gtccccgggt gacctcctcg tcctcgtact gatcgctcat ggaaagacct
     1921 gtggtgcggt cttagtcccg aacggcgttt tgcgggaggt gcgagcgccg aattagcgtt
     1981 ggcgaggcgg gcgggtcaca ggaagtaggg gagtcgttcg acgggttcgc ggcgccggcg
     2041 ccgcgccgac ccattgctac tccctacgcc ctgcgccctt cttccggccg cccccgaacg
     2101 ggcgccagcg caggaagccg atgatcgccg gcccgaccac cggcgtgcgc agcagcggac
     2161 gcacggcgtg cggccgggtc ttcaggaaac agaccaccgc cccgatccag aagacggtgg
     2221 tcggcagtcc gggcgtgacg acgccgaccg cgcccaccgc cacgagagtc gcgccaaggc
     2281 cgtcgagcgc ggcccgggcg gcgggcgatc ggtggcggtt gggtggcgga cgctccatgg
     2341 cggcgactat tgcgggttcc gccgctttga gccaccacaa ccccatgtcc tcgatcctgc
     2401 ccgacgccgc gccgaccaac tgggtcgatc gccacgcccc cgccggcttg cgaccgtggc
     2461 tgaagctcgg gcggttcgac cggccgaccg gcatctggct gttgatgctg cccggctgga
     2521 tgggcatcgc cctggccgcc gccatgcggg gcaaggcgcc ggacctcgtg ttgatgttgc
     2581 tgttcttcat cggggcggcg ctgatgcggg cggcgggctg cgcctacaac gacatcgtcg
     2641 accgcgacat cgacgccagg gtcgcccgca ccgccggccg gccgatcccg gcgggccaga
     2701 tcagcgtcag acaggcctgg gccttcgtca tcggctgctg tctggtcagc ctgctgatcc
     2761 tgctggccat gaactggtgg accatcggcc ttggcgtcgc ctcgctggcg ctggtggcgg
     2821 cctatccgtt catgaagcgg atcacctggt ggccgcaggc ctggctgggc ctgaccttca
     2881 actggggcgc cctgatgggc ttcgcggccg tcgccggcat gcccggctgg ccggctgtgc
     2941 tgctctacgc cggcctggtg ttctggaccc tcggctacga caccatctac gcggtgcagg
     3001 acctggagga cgacgccctg gccggggtca agtcctcggc cctgcggctg ggagagcagg
     3061 cgccgcgggc ggtgctgctg ttctacgggg tctgcttcgg cctggtgctg acgggcgcct
     3121 gggtcggcgg gctgggaccg ttgttcctgc cgccggcggc cctgttcgcg ctgcacctgt
     3181 cgcggcaggc gtcgagactc gacgtcgccg acccggccgg ggccctggcc ctgttcaagt
     3241 ccaacagcct ggccgggctg gtgctggtgg tcgcgctggc ggccggagcc tggaagccgg
     3301 gcgcagtctt ctagaattct cccctctgcg aggggaggac caaggctaga acggcccgtt
     3361 cggatagacg cgcgtctcca gcacccggtt cagccgggcg atgcgcgtgg cgtaggaccg
     3421 cgaaagacag gcgcgatcgg ggccgcaggt ccgccgctgc cgcaaccagc gggactgttc
     3481 gtccatgatc gcgccgcgcg atcccatcgg gaccagacgc cgcacgatat cgtagagcag
     3541 ggccatgcgc acgtcctggt cgttgagcgc gcgatcgtcg cagatggcgc gctcgtcggg
     3601 cgtcgcggcc ctggcgcagt tgaaactggc ggcgccggcc gccccggcgc ccagaatgcc
     3661 ggccgccaga acggccgcga acaggcgaac acgcatggaa ccctccaggc cgtgacgcgg
     3721 cgtcatccta cacatcgcgg cgatgaggtg agataatcac ggcatggccg tcgccgcacg
     3781 cgtgaaaccg aaggacttct tcaccgacga ggagtgggcc cccctggccg cccgctcgtc
     3841 gtggaagggg ctgtggctgg tcgcccacgc ctggctgacc atcctggccg ccggcgcgct
     3901 gttcgtgatc tggcccaacc cgctgaccct cctgctggcg gtgatgatca tcggcgcgcg
     3961 gcagctgggc ctggccatcc tgatgcacga cggcgcccac ggctgcctgc acccgaacca
     4021 gaaggtcaat gactgggtcg gccactggct gtgcggcgcc ccgaccaacg gcgacctgcg
     4081 gcgctatcgc gactaccacc tgcagcacca caagttcgcc cagcagcagg aggacccgga
     4141 cctcggcctg tccgcgccgt tcccgatcac ccgccagtcc ctgtggcgca aggccgtccg
     4201 cgatctgacc ggccagacct tctacaagca gcgcatcaag ccgagcctcg acgcgttccg
     4261 caccggggcg aagaagggcg tccccttcgg caagaccgcg aagggcgtgc tgaagttctg
     4321 gtcgccgttc ctgatcttcc acctcccggt gatcgccgtg ctgtcggcgt gcggcctgtg
     4381 gtgggcctgg cccgtgcttt ggctgctgcc catggcgacc tggtatccgc tgatcacccg
     4441 cctgcggaac atcgccgagc acgccctggt cggggtgaac cagcccgacc cgttgcgcca
     4501 cgcccgcacc accctggccg gcccggtcga gcgcctgatc ctcgcgccct actacgtcaa
     4561 ctatcactgc gaacatcaca tgttcatgca cgtgccctgc tggaacctgc cgaaggtcca
     4621 caggctgctg aagcagaagg gcgtgctgga gaagatgctg accgcgccgg gctatgtggc
     4681 ggtgctgcgg gaggcggccg gccgccttga agctcagcct gcctgaacca tccggaccag
     4741 catccagacg gccatcgcga tcatcatcag gttttccgtc agcgagacga agcccagcgg
     4801 cacgttgctg tcgccgccga cgcaggcgca cttcaggtcg cgcttctgga tgtagacggc
     4861 cttgaacacc gacaccgccc cgatgacgcc gatgaacagc gcgaccggcg ctgccagcca
     4921 agtcaggacg ccggcgatca tcagcacgcc cgcgcccagt tcggcgaacg gatagacata
     4981 gccatacgga acccaccggc gcgccagcag gtcgtagccg aggaacatgg tcgagaagct
     5041 ggccaggtcg cggagcttga gcatggccag cagacacatg ctgaaggcga tgaaccattc
     5101 gccggcccga agggtcagaa gggtcccgaa cgcagcctgg ctcgccgcca gagtcatcag
     5161 cgcggcgacc gcgaacaccg ccaggacggg agtgtaggtc ttctggtccg ggtcacggac
     5221 cttcagcccg aagaaccggc gcaggtcgtc gtagccgccg atccgcctgc cgtcgatgaa
     5281 ggtctggggc gtggtctcaa ccttgtgccc agccttgaag gcgtcggtct cctcgcgggt
     5341 ccgcagccag tggtcatcga tcacatagcc cttgctgtgg agcaggtgtc tggacttcag
     5401 cccccacggg cagacgtgct tctccatcac catccggtag agggtggcct gcttccgagg
     5461 gggtccatcc gtcatcgtcg gtctcccttg ccctcgatcg ataacgcacg agtcggacga
     5521 ccagacccac gatagaacgt ggtcaacggg ccctatccgg gccaagacgc gcccatgatc
     5581 accgaccgcc gcgccttcat cgtcgagaac acccgcctcc aggcgccgcc ccatgcgccc
     5641 gagctggtgc tgcacctggc cgacgagatc accccgatct ggaaactgac cgaggaggcg
     5701 ctggccgaga tcggcctgcc gccgccgttc tgggccttcg cctgggccgg cgggcaggcg
     5761 ctcgcgcgct atgtcctgga caacccggac atcgtcgccg gcaagcgggt ggtggacttc
     5821 gcctccggct cgggaatggt cgccatcgcg gccatgaagg cgggggcggc ctcggcgctg
     5881 gcggcggaca tcgacgtctt ctgcgaggcg gcggtggcgc tgaacgcggc ggcgaacggc
     5941 gtggtggtgg atttcaccga tctggacctg ctggacgctc cggcgcccga ctgggccgag
     6001 gtgatcctcg ccggcgacat ctgctacgag aagccgctgg ccgagcgggt gatcgcctgg
     6061 ctgaccgcgg cccgggcggc cggcaagaca gtgctgatcg gcgatccggg ccgcacctat
     6121 tttcccagga ccggcctgac cctgctggcc gagtaccagg tgccgaccac ccgcgaactg
     6181 gaggacatgg aggtcaagcg cacccgcgtc tggaccttcc cttgaccgcg aggtcgggtt
     6241 gtggaacaaa acaggaactc aaggaggtcg cccgatgcgc gaagacggca agatcgacta
     6301 tgtggaattc cccgccgatg gcattccggc ggtgaaggac ttctacagca aggccttcgg
     6361 ctggacctgg gtcgactacg gaccggacta cgccgccttc ggcgacgcgg ccgtggacgg
     6421 cggcttcaac ggcgacagca ccgacgccgg ccgggcggcc gggccgctgg tggtgctcta
     6481 cgcccatgac atcgaggcca tggccgacag ggtgaccgcc gccggcggtg tgatcatcaa
     6541 cccgatctac agcttccccg gtggccggcg gttccacttc accgacccgt ccgggaacga
     6601 gctggcggtt tggagcgaga cgtagggagt agggagtagg gagtagggag tagttccgcc
     6661 tgctcacgcc gccgccgcgc ctgccctatg gctactccct accccctact ccctactccc
     6721 taacctctag ctccttcccc ccaccggcct atagtccccg cgcacagtgt ccccggggag
     6781 tcccgcccat gcgtcgtcgc acctttctcg ccgccctgcc ggccgccgct ttcgcgggtc
     6841 aggccgcggc ccagagccgg ccgaccaatc ccaacctgaa ccggcccgac gtgcacggcg
     6901 gcgaccgcat cgatggcgcg accttcgcca gc
//
LOCUS       DAORNX010000115         6926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8097_length_6926_cov_7.380148, whole genome shotgun sequence.
ACCESSION   DAORNX010000115 DAORNX010000000
VERSION     DAORNX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_6926_cov_7.380148"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1851)
                     /locus_tag="PLO65_06645"
     CDS             complement(100..1851)
                     /locus_tag="PLO65_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRD27961.1"
                     /translation="MTDAAQSEKPWPVMTVREAYAILTAPGMPTEMEEVVIRGIPTRV
                     WKNAPATIRDVVAIGRAHGDKVFLVHENERVTFEAFHRAVAAFAQALADRGVVKGDRV
                     AIVMRNLPEWPVAFYAAASLGAIVTPLNAWWTGPELEYGLTDSGAKVCVVDVERFDRL
                     SEHFPNCPDLRHVFVSRMTDEVAHPFVSKLEDAIGGPNDWATLPDAPLPTTPIEADDD
                     ATIFYTSGTTGKPKGALATQRNVNSNIFSALCAGARPFLRRGETPPAPDPTAPQKGSL
                     LSVPFFHATGCFAVLNPTLYAGGKLAMMRRWDPELAMQIIERERLTGAGGVPTIAWQL
                     IEHPARAKYDLSSIESMAYGGAPSAPELVRKIKEVWPKSHAGNGWGMTETSATATSNS
                     AEDYEHRPDSCGPPVPVTDLKIMTVEPPFTELPIGAVGELWCKGPQVVKGYWNKPEAT
                     AQTFVDGWVRTGDLARLDEEGFCFIIDRAKDMLIRGGENIYCIEVENVLYDHPAVMDA
                     ALVGIPHKTLGEEPGAVVTLKEGAHATEQELRAFVADRLAAFKVPVKVVFWHETLPRN
                     ANGKILKNELKKVFEAA"
     gene            2037..2534
                     /locus_tag="PLO65_06650"
     CDS             2037..2534
                     /locus_tag="PLO65_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD27962.1"
                     /translation="MTAFPLHTRATAPFEAKPFLEQAGQSIGYVPALLAQMAEAPALL
                     EGYAALSAIFRKSGLSPREQQLVLRTAAVALGSRYCTPAHVARAREAALDEDAIADAR
                     AGRPLVDRRLEALRRFTQVMVRAGRPQPVPRRRLHACQRPGSRAGRRAQDPVRQRRPD
                     GADPA"
     gene            2596..3045
                     /locus_tag="PLO65_06655"
     CDS             2596..3045
                     /locus_tag="PLO65_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267470936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HRD27963.1"
                     /translation="MKRLLLVLVLATAALPGAALAAKPPVYTAPFSQLAAGGYDVVAY
                     FTVGHPVKGDPKFATDWKGARWQFSSAANLAKFRASPASYAPQYGGYCAWAVANGYTA
                     KGDPLNWKIVGGKLYLNYNDEIQSRWVKDIPGFIRKGDGNWPTVLTK"
     gene            3054..3533
                     /locus_tag="PLO65_06660"
     CDS             3054..3533
                     /locus_tag="PLO65_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27964.1"
                     /translation="MNRVLKYLPDLMAVFMAVIFLDSLRYKFTDHPKTQVIFGLLDGW
                     ATGLGAPGLFGHTGLFSQYVIGSIELLASTLLIVGLHPRFKHLQAGGALAGLLVMTGA
                     VSFHLFTPLGTDPNHDGGGLFIAACTNLAFAVLLLAVFRRAALAELGRRVAAVLRPY"
     gene            complement(3616..6318)
                     /locus_tag="PLO65_06665"
     CDS             complement(3616..6318)
                     /locus_tag="PLO65_06665"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRD27965.1"
                     /translation="MLDKTFDPQTVEPRLYRAWEESGAFAPTDDPAAEPFSIVIPPPN
                     VTGSLHIGHALNNTLQDVLIRFQRMRGKAALWLPGTDHAGIATQMVVERQLAAAGNMG
                     RREMGREAFVAKVWDWKAESGGVIVNQLRRLGASCDWSRERFTLDEGLSAAVRKVFVT
                     LYKQGLIYRDKRLVNWDPQFQTAISDLEVEQKEVDGAYWHFAYPLENGVTYRYPVAFD
                     EDGKATEWETRDYIVVATTRPETMLGDTAVAVHPDDERYRDLVGKTVKLPIVGRSIPI
                     VADDYADPTKGSGAVKITPAHDFNDFEVGKRHGLEAISILDAFGRINDNAPEAYRGHD
                     RFVARKMVIEAFEHLELLKGIEKTRHMVPHGDRSGVVIEPFLTDQWYVDAETLARPAI
                     KAVEEGATVFEPANWDKTYFEWMRNIQPWCISRQLWWGHRIPAWFDDQGNVYVEETEE
                     AARAAAGGKALTQDEDVLDTWFSSGLWPFSTLGWPEKTADLARFYPTHTLVTGFDIIF
                     FWVARMMMMGLHFTGEVPFQRVVINALVRDASGAKMSKSKGNVMDPLELIDQYGCDAV
                     RFTLTAMSGQARDIKLSTQRIEGYRNFGTKLWNATRFAQMNGCMRVEGFDPAGVEQTI
                     NKWIRGEAVRTAAEVTRALDACAFSDAASALYRFIWNVYCDWYVELAKPILNGSDEAA
                     RAETQAMTAWVLDQCLLLLHPVMPFITEELWDKTAEGGPARDGFLMTARWPDLPEAWI
                     DSRAEAEIGWIVDLVTEVRSIRAEMNVPPGARVPLTLSGMSEETRARLARHRDLILTL
                     GRLGSAEEGEPPAGSAPFTLGEATAAIAIADFIDLAAERARLTKEIAGHAGDIERVNR
                     KLGNPDFLAKAKEEVIEENREKLAEAEAAKARLEAALGRLQSVG"
     gene            complement(6430..6894)
                     /locus_tag="PLO65_06670"
     CDS             complement(6430..6894)
                     /locus_tag="PLO65_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRD27966.1"
                     /translation="MEEILASIRRIISEDDAPPAEAPAAFEEPAVAMASAPTPAPVEP
                     DDDDVLELTETIEAADDIETVGDLDVYSAPKAAPEPAWEPEPEPEPVFAAAPEPAPAP
                     SYTTGSASISGGTTIEELVRSILEPKLQEWADQNLDGIVRSILREKLDRVFR"
BASE COUNT          983 a         2366 c         2399 g         1178 t
ORIGIN      
        1 ccctggaggc cgctcgcccc tgggccgggc gcaagccgcc ggtcagcgcg gggtagtcca
       61 ggcggggaca cgtactggcg gtacgtgtcc cctcgcccgc tacgccgcct cgaacacctt
      121 cttcagctcg ttcttgagga tcttgccgtt ggcgttgcgc ggcagggtct cgtgccagaa
      181 gaccaccttc accggcacct tgaaggcggc caggcggtcg gcgacgaagg cgcgcagttc
      241 ctgctccgtc gcgtgggcgc cctccttaag ggtgacgacc gcgccgggtt cctcgcccag
      301 cgtcttgtgc gggatgccga ccagggccgc gtccatcacc gcggggtggt cgtagaggac
      361 gttctcgacc tcgatgcagt agatgttctc gccgccgcgg atcagcatgt ccttggcccg
      421 gtcgatgatg aagcagaagc cttcctcgtc gagccgcgcc agatcgcccg tgcggaccca
      481 gccgtcgacg aaggtctggg ccgtggcttc cggcttgttc cagtagccct tcacgacctg
      541 cgggcccttg caccacagct cgccgaccgc accgatcggc agttcggtga acggcggctc
      601 gacggtcatg atcttcaggt cggtgaccgg caccggcggg ccgcagctgt ccgggcggtg
      661 ctcgtagtcc tcggccgagt tggaggtcgc cgtggcgctg gtctcggtca tgccccagcc
      721 gttgccggcg tgggacttgg gccagacctc cttgatcttg cgcaccagct cgggggccga
      781 gggggcgccg ccgtaggcca tgctctcgat cgagctgagg tcatacttcg cgcgcgccgg
      841 atgctcgatc agctgccagg cgatggtcgg cacgccgccg gcgccggtca gtcgctcgcg
      901 ctcgatgatc tgcatcgcca gctccggatc ccagcgccgc atcatcgcca gcttgccgcc
      961 ggcgtagagg gtcgggttga gcacggcgaa gcagccggtg gcgtggaaga agggcacgct
     1021 gagcagactg cccttctgcg gggccgtcgg atccggcgcg ggcggggtct cgccgcgtcg
     1081 caggaacggc cgggcgccgg cgcagagggc ggagaagatg ttcgagttga cgttgcgctg
     1141 cgtcgccagg gcgcccttgg gcttacccgt cgtgccggag gtgtagaaga tggtcgcgtc
     1201 gtcgtcggcc tcgatcgggg tcgtcggcag cggcgcgtcc ggcagggtcg cccagtcatt
     1261 ggggccgccg atcgcgtcct ccagcttgct gacgaacggg tgggcgacct cgtcggtcat
     1321 ccggctgacg aacacgtgcc gcaggtcggg acagttcggg aagtgctcgg agagacggtc
     1381 gaaccgctcg acatcgacga cgcagacctt ggcgcccgag tcggtcaggc cgtactcgag
     1441 ctccggtccc gtccaccagg cgttcagcgg cgtgacgatc gcgccaaggc tggccgcggc
     1501 gtagaaggcc accggccatt cgggcagatt gcgcatgacg atggccaccc ggtccccctt
     1561 gaccacgccc ctgtccgcca gcgcctgcgc gaaggccgcg accgcgcggt ggaaggcctc
     1621 gaaggtgacg cgctcgttct cgtgaaccag aaagaccttg tcgccgtgcg ctcggccgat
     1681 ggcgaccacg tcgcggatgg tcgccggggc gttcttccac acccgggtcg ggatgccgcg
     1741 gatgacgacc tcttccatct cggtgggcat gccgggcgcg gtgaggatgg cgtaggcctc
     1801 ccgcacggtc atcacgggcc acggcttctc ggactgggcg gcgtcggtca tgcgaattct
     1861 ccctgcaact ggctggacgt tcttggcgac gcttgggccg tccaaagcac agccggggcg
     1921 ggcttgcaaa cagctgtttg agaccccgtg gtcggcgcga tccatgccgg gcgtgcatgg
     1981 tgacgatgcc ggccgccggg attacgctcg tgacccaatt cgggcaggct cgggtcatga
     2041 ctgcgtttcc gctgcacacc cgcgccaccg ccccgttcga ggccaagccg ttcctcgaac
     2101 aggccggcca gtcgatcggc tacgtgcccg ccctgctggc ccagatggcc gaggctccgg
     2161 ccctgcttga aggctatgcc gccctgtcgg cgatcttccg caagagcggc ctttcgccgc
     2221 gcgaacagca gctggtgctg cggacggccg ccgtggcgct gggcagccgc tattgcacgc
     2281 cggcccacgt ggcccgggcg cgcgaagccg ccctggatga ggacgccatc gccgacgccc
     2341 gggccggccg gccgctcgtc gatcgccggc tggaggcgct gcggcgcttc acccaggtca
     2401 tggttcgggc cggacgacct cagcccgttc ctcgacgccg gcttcacgcc tgccaacgcc
     2461 ctggaagtcg tgctgggcgt cgggctcaag accctgtccg ccagcgccgc ccggatggcg
     2521 cagaccccgc ttgatccgcc gaaaatcgcc tacgccctga ccctgggtcg gcgtttggcc
     2581 tgaaggagac ccgccatgaa acgcctgttg ctcgtcctgg tcctcgccac cgccgccctc
     2641 cccggcgcgg cgctggccgc caagccgccg gtctacaccg cgcccttctc gcagctcgcc
     2701 gccggcggct acgacgtggt ggcctatttc accgtcggcc atccggtgaa gggcgacccg
     2761 aaattcgcca ccgactggaa aggcgcccgc tggcagttct cgtcggccgc caacctggcg
     2821 aagttcaggg ccagcccggc cagctatgcg ccgcaatacg gcggctactg cgcctgggcc
     2881 gtggccaacg gctacacggc caagggcgat ccgctgaact ggaagatcgt cggcggcaag
     2941 ctctacctca actacaatga cgagattcag tcccgctggg tcaaggacat ccccggtttc
     3001 atccgcaagg gcgacggcaa ctggccgacc gtcctgacca agtgagcgcc accatgaacc
     3061 gcgtgctgaa atacctgccc gacctgatgg ccgtgttcat ggccgtcatc ttcctcgaca
     3121 gcctgcgcta caagttcacc gaccatccca agacccaggt gatcttcggc ctgctcgacg
     3181 gctgggcgac cggcctgggg gcgccggggc tgttcggcca caccggactg ttctcgcagt
     3241 atgtcatcgg ctcgatcgaa ctactggctt cgacgctgct gattgtcggg ctgcatccca
     3301 ggttcaagca cctgcaggcc ggcggcgccc tggccgggct gctggtgatg accggcgccg
     3361 tcagcttcca cctgtttacg ccattgggga cggacccgaa ccatgacggc ggcggcctgt
     3421 tcatcgccgc ctgcaccaac ctggccttcg ccgtgctgct gctggccgtg ttcaggcgcg
     3481 cggcgctggc cgagctgggg cggcgggtgg cggcggtgct gcggccatac tgaagcgagc
     3541 acggggacat gcacttcagt gcgtgtcccc ttcagccgaa cagggacacg tactgacgta
     3601 catgtccctg agcccctacc ccacgctctg cagccgcccg agcgcggctt cgagccgggc
     3661 ctttgcggcc tcggcctcgg cgagcttctc gcggttctcc tcgatgacct cttccttggc
     3721 cttggccagg aagtcggggt tgccgagctt gcggttgacc cgctcgatgt cgccggcgtg
     3781 gccggcgatc tccttggtca ggcgcgcgcg ctcggcggcc aggtcgatga agtcggcgat
     3841 ggcgatggcg gccgtggcct cgcccagggt gaagggcgcc gatccggccg gcggctcgcc
     3901 ctcctcggcc gagccgagcc ggccgagcgt caggatcagg tcacgatggc gcgccagccg
     3961 cgcccgggtc tcctcgctca tgcccgacag ggtcaggggc acgcgggcgc ccggcggcac
     4021 gttcatctcg gcgcggatcg agcgcacctc ggtgaccagg tcgacgatcc agccgatctc
     4081 ggcctcggcc cgcgagtcga tccaggcctc cggcaggtcg ggccagcgcg ccgtcatcag
     4141 gaatccgtcg cgggccgggc cgccctcggc ggtcttgtcc cacagctctt cggtgatgaa
     4201 cggcatgacc gggtgcagca gcagcagaca ctggtcgagc acccaggcgg tcatggcctg
     4261 ggtctcggcc cgggccgcct cgtccgaccc gttcaggatc ggcttggcca actcgacata
     4321 ccagtcgcag tagacgttcc agatgaagcg gtagagcgcg ctggccgcgt cgctgaaggc
     4381 gcaggcgtcg agcgcgcgcg tgacctcggc ggccgtcctg accgcctcgc cgcggatcca
     4441 cttgttgatg gtctgctcga cgccggccgg atcgaagccc tcgacgcgca tgcagccgtt
     4501 catctgggcg aagcgggtgg cgttccacag cttggtgccg aaattacgat atccttcaat
     4561 gcgctgggtc gacagcttga tgtcacgcgc ctggcccgac atggccgtca gggtgaagcg
     4621 caccgcgtca cagccgtact ggtcgatgag ctccagcggg tccatgacat tgcccttgga
     4681 cttgctcatc ttggccccgg acgcgtcgcg caccagggcg ttgatgacca cccgctggaa
     4741 cggaacctcg ccggtgaaat gcaggcccat catcatcatc cgggcgaccc agaagaagat
     4801 gatgtcgaaa ccggtgacca gggtgtgggt cgggtagaac cgcgccaggt cggcggtctt
     4861 ctccggccag cccagcgtcg agaacggcca caggccggac gagaaccagg tgtccaggac
     4921 gtcctcgtcc tgcgtcagcg ccttcccgcc ggcggcggcg cgggccgcct cctcggtctc
     4981 ctcgacgtag acattgccct ggtcatcgaa ccaagccgga atccggtggc cccaccacag
     5041 ctggcggctg atgcaccagg gctggatgtt gcgcatccac tcgaagtagg tcttgtccca
     5101 gttggccggc tcgaagacgg tcgccccctc ctcaacggcc ttgatggcgg gcctggcgag
     5161 cgtctcggcg tcgacgtacc actggtcggt caggaagggc tcgatgacca cgcccgagcg
     5221 gtcgccgtgc ggcaccatgt ggcgggtctt ctcgatgccc ttgagaagct caaggtgttc
     5281 aaaggcttcg atgaccatct tgcgggcgac gaagcggtcg tgaccgcggt aggcctccgg
     5341 cgcgttgtca ttgatccggc cgaaggcgtc gaggatgctg atcgcttcca gcccgtggcg
     5401 cttgccgacc tcgaagtcgt tgaagtcatg cgcgggcgtg atcttcaccg cgcccgagcc
     5461 tttggtcggg tcggcgtagt cgtcggcgac gatcgggatc gagcggccga cgatcggcag
     5521 cttcacggtc ttgccgacca ggtcccggta gcgctcgtcg tccgggtgaa cggcgacggc
     5581 cgtgtcgccc agcatggtct ccggccgcgt ggtggcgacg acgatgtagt cgcgcgtctc
     5641 ccactcggtg gccttgccgt cctcgtcgaa ggcgactgga taccggtagg tgacgccgtt
     5701 ctcgagcgga taggcgaagt gccagtaggc gccgtcgacc tccttctgct cgacctcgag
     5761 gtcggaaatg gccgtctgga actgcgggtc ccagttcacc agccgcttgt cgcggtagat
     5821 caggccctgc ttgtagagcg tgacgaacac cttgcgcacg gcggcggaca ggccctcgtc
     5881 gagggtgaag cgctcgcgcg accagtcgca gctggcgccc aggcggcgca gctggttgac
     5941 gatcaccccg ccgctctcgg ccttccagtc ccagaccttg gcgacgaagg cctcgcggcc
     6001 catctcgcgg cggcccatgt tgcccgccgc ggccagctgg cgctcgacga ccatctgggt
     6061 ggcgatgccg gcgtggtcgg tgcccggcaa ccacagggcc gccttgccgc gcatgcgctg
     6121 gaagcggatc agcacgtcct gcagcgtgtt gttcagcgcg tggccgatat gcaggctgcc
     6181 cgtgacgttg ggcggcggga tgacgatgct gaacggctcg gcggccgggt cgtcggtggg
     6241 ggcgaacgcg ccggactctt cccacgccct gtagaggcgc ggttcaacgg tctgggggtc
     6301 gaaggtcttg tcgagcatgg aattggacac gcaaaagggc ggcgcgctgg ctgcgggccg
     6361 ccggtctcta tcaaaggtga tcgggatcgc gaagcggcaa ggccggcgtg gcgcgccggc
     6421 ctctatggcc tagcggaata ctcggtcgag cttttcgcgc aggatcgagc ggacgatgcc
     6481 gtccaggttc tggtcggccc attcctgcag cttgggttcc aggatgctgc ggaccagctc
     6541 ctcgatcgtg gtgccaccgg aaatcgacgc cgagcccgtc gtgtagcttg gggccggcgc
     6601 cggctccggc gccgccgcga agacgggttc aggttcaggt tcgggttccc aggcgggctc
     6661 cggcgcggcc ttcggcgcgc tgtagacatc aaggtccccg accgtctcga tatcgtcggc
     6721 ggcctcgatc gtctcggtca gctcgagaac atcgtcgtcg tcaggctcga ccggcgcggg
     6781 cgtgggcgcg gaggccatgg ccacggccgg ctcctcgaac gcggccggcg cctcggcggg
     6841 cggcgcgtcg tcctcggaga taatgcgccg gatggaggcg agaatctcct ccatcgtcgg
     6901 ttcctgggcg gtctgttcgg acatgg
//
LOCUS       DAORNX010000116         6901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8148_length_6901_cov_8.566900, whole genome shotgun sequence.
ACCESSION   DAORNX010000116 DAORNX010000000
VERSION     DAORNX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8148_length_6901_cov_8.566900"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLO65_06675"
     CDS             <1..286
                     /locus_tag="PLO65_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRD27967.1"
                     /translation="PDGDPRAAALLAAFEPPPEYLALQAVVERMTGEAANVDFAVTAL
                     CARFGLPADAPFALFAVARTAGWLAHAMEQVQTGTLIRPRARYIGESPPE"
     gene            complement(259..1446)
                     /locus_tag="PLO65_06680"
     CDS             complement(259..1446)
                     /locus_tag="PLO65_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HRD27968.1"
                     /translation="MTTKPAPDIGALVRAAEAELRLRNSTAALELLARAEAAAPADIP
                     VKMLKASALRLAGDLPGALAALEGVLTQDPYHFLALLSKGALLERLVGPRIAARFYRD
                     ILEAAPPEERIPPGLAAPIAHARKLVAEMSDDLADFLRQRVSTVKARHGNASLARFEE
                     ALDIYAGRRKPFVQQPLLMHFPQLPAIPFHDRAHFPWLPELEAATDMIRGELDVALDV
                     AHEDFAPYVAYPPGTPVGQWRDLNNSTNWRSLWLWKDGRRQDQACAICPGTAALLDTL
                     PMADQPGFAPTALFSALEAHTRIPPHTGSTNTRLLVHLPLILPGPAGFRVGNETRAWR
                     MGEAWVFDDSIEHEAWNDADALRVILIFDVWNPFLSEAERDLLTVMMNARNAYYSGGD
                     SPM"
     gene            complement(1581..6119)
                     /gene="gltB"
                     /locus_tag="PLO65_06685"
     CDS             complement(1581..6119)
                     /gene="gltB"
                     /locus_tag="PLO65_06685"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRD27969.1"
                     /translation="MSDALDLYLKNRQRLIDGHAYDPSSERDNCGVGLVCAIDGKPRR
                     EVVDLAIKALKAVWHRGAVDADGKTGDGAGILLSVPQDFFADQVRNTGHKLRPGAIAV
                     GQVFLPRTDLGAQEAARTIVESEALRFGFYIYGWRQVPVDTSVIGEKANATRPEIEQI
                     MLSPPSGMEGEALERALFLCRKRIEKKVAAAAINDFYICSLSARSLIYKGMFLAENID
                     DFYPDLRDERFAAAVAIFHQRYSTNTFPQWRLAQPFRMLAHNGEINTLKGNTNWMKSH
                     EIRMAAQAFGEFGDDVKPVIQPSGSDSAALDNTYEILVRAGRSAPMAKALLIPEAWAT
                     RSDEQMKPEHRALYSYCNAVMEPWDGPAALCATDGRWVVAGKDRNGLRPLRVAYTDDG
                     LVIMGSEAGMCGVEETRIVRKLNISPGRMIAIDLVEGRLYDEEAIIDRLAGAHPYTDW
                     LGNMVELESRIGPGPEPRRFAGEELARRQAAAGMTLEDLELVLAPMVEDGKEAIGSMG
                     DDTPLAVLSDNYRPLSHYFRQNFSQVTNPPIDPLRETGVMSLKTRFKNLGNILVEDEA
                     QTDVYVLESPVLTTGMYERLLGYIGDKALAVIDCTMPIPADEARPGDALRANLDRIRA
                     EAEDAALRGCATIVLTDEHSTAERVALPMILATGGVHAHLVGKGLRSYVSIIVRSAEC
                     MDTHYFAVLVGVGATAVNAWLSQESFQDRLDRGLLGEATLRDTCVNFKTAIEQGLLKI
                     ISKMGIAVISSYRGGYNFEAVGLSRSLVAEFFPGMPSRISGIGFAGLEKRAVELHRRA
                     WDPAFIALPVGGFYKARRSGEAHAFEARMIHTLQRACDTGDYETYRRFSEGMRTLPQI
                     QLRDLLDWRSDRKPLNPDEVESVNEIRKRFLTPGMSLGALGPEAHGALNIAMNRIGAK
                     SVSGEGGEDRARYRPLPNGDNPNSAVKQVASGRFGVTAEYLNECREIEIKVAQGAKPG
                     EGGQLPGFKVTEMIARLRHATPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDARVT
                     VKLVSMTGIGAIAAGVAKAKADVILISGHVGGTGASPQTSIKYAGSPWEMGLSEANQV
                     LTLNNLRHSVRLRTDGGIRTGRDVVIAAMLGAEEFGIGTASLIAMGCIMVRQCHSNTC
                     PVGVCTQDEALRARFTGTPDKVVNLFTFIAEEVRAILARLGFRSIQEIVGRTDLLTQV
                     SRGGEHLDDLDLNPLLVRADPGSNKPYCTVEGRNEVPDTLDAQIVRDAAPLLQRGEKM
                     QLTYTVRNTARAIGARISSHITRKFGMSALPSDHLTLELKGSAGQSLGAFAVRGLRIV
                     LTGEANDYVGKGLSGATIVVRPSSHLASPESNAIIGNTCLYGATAGKLFAAGQAGERF
                     AVRNSGAVTVVEGCGANGCEYMTGGAAVILGPTGGNFGAGMTGGMAYVLDLAGRFRGR
                     VNPDSVIVQRLASPHWEEHLRRLIAEHAHETGSAFAQGILRDWDRMRGQFWQVCPKEM
                     AGRLDHPLESEEAAFERA"
     gene            complement(6125..>6901)
                     /locus_tag="PLO65_06690"
     CDS             complement(6125..>6901)
                     /locus_tag="PLO65_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD27970.1"
                     /translation="TLPQLRERHDAVLLATGVYAARELEMPGAGSDGVVAALDYLIAS
                     NRKGMGDMVADFEAGTLNAADKDVVVVGGGDTAMDCVRTAVRQGARSVTCLYRRDREN
                     MPGSMREVTNAEEEGVVFEWLAAPRGLLGDADGVTGVRAIRMRLGPPDSSGRRAPEPV
                     DGSDFDLPARLVIKALGFDPEDLPATLGQPDLAISRWGTVKIDRRTQAANLDGVFAAG
                     DIARGASLVVWAIRDGRDAADSIHSYLRAKADSPALVAAE"
BASE COUNT         1016 a         2335 c         2456 g         1094 t
ORIGIN      
        1 cccggatggc gaccctcgcg cggccgccct gctggccgcc ttcgaaccgc cgccggagta
       61 cctcgccctc caggcggtgg tcgagcgcat gaccggcgag gccgccaacg tcgacttcgc
      121 ggtcaccgcc ctgtgcgcgc ggttcggcct gcccgccgac gccccgttcg ccctgttcgc
      181 ggtcgcgagg acggcgggct ggctggccca cgccatggag caggtccaga ccggcaccct
      241 gatccgcccc cgcgcccgct acatcggcga gtcgccgccg gaataatagg cgttgcgggc
      301 gttcatcatg accgtcagca agtcgcgctc cgcctcgctg aggaacggat tccagacatc
      361 gaagatcagg atcacgcgca gcgcgtctgc gtcgttccac gcctcgtgct cgatggagtc
      421 atcgaacacc catgcctcgc ccattcgcca ggcgcgcgtc tcgttgccga ctcgaaatcc
      481 tgccgggccc ggcaggatca gcggcagatg caccagcagg cgagtattgg tcgatcctgt
      541 gtgcggcggg atacgcgtat gggcctcaag cgccgaaaac agggcggttg gcgcaaatcc
      601 cggctggtcg gccatgggca acgtgtcgag cagggcggcc gtcccgggac agatcgcgca
      661 ggcctggtcc tgccggcgac cgtccttcca caaccaaagc gaccgccagt tcgtcgagtt
      721 gttcaggtcg cgccattggc cgaccggcgt cccgggtgga taggcgacgt acggcgcgaa
      781 atcctcgtgg gcgacgtcca gcgcgacgtc cagttcgccg cggatcatgt cggtggccgc
      841 ctccagctcc ggcaaccagg gaaagtgggc ccggtcatgg aacgggatcg cgggcagttg
      901 tgggaaatgc atcagaagcg gctgctggac gaacggcttg cgacgccccg catagatgtc
      961 gagagcctcc tcgaaacgcg ccagtgaggc gttgccgtgc cgcgccttca ccgtgctgac
     1021 ccgctggcgc aggaaatccg ccagatcgtc cgacatctcg gccacgagct tgcgggcgtg
     1081 ggcgatgggg gcggcaagac cgggcgggat ccgctcctcc ggcggcgcgg cttcaaggat
     1141 gtcgcgatag aaccgtgcgg cgatgcgcgg acccaccagc cgctccagca gcgctccctt
     1201 cgagagaagc gcaaggaagt gatagggatc ctgggtcagg acgccttcga gcgcggcaag
     1261 cgcgcccggc agatccccgg ccaggcgcag ggccgaggcc ttgagcatct tgacgggaat
     1321 gtcggcgggc gccgccgctt cggcgcgtgc gagcagctcc agcgcggccg tcgagttccg
     1381 cagccggagc tccgcctccg ccgccctgac cagcgcgcca atgtccggcg ccggcttcgt
     1441 tgtcatgtcg ccccctggac ggggccgttc gaagacccgc gttccgcgac aagctatggg
     1501 cagccttcag agcgatcgaa cggaaagatg acaaatcggt aatggtgcga ccacacgcct
     1561 cagcccaatt cgggtcggat ctacgcgcgt tcgaaggccg cttcctcgga ctccagcgga
     1621 tggtcgagcc ggccggccat ctccttgggg cagacctgcc agaactgccc gcgcatgcgg
     1681 tcccagtcgc gcaggatgcc ctgggcgaag gccgaaccgg tttcgtgggc gtgctcggcg
     1741 atcagccggc gcagatgctc ttcccagtgg ggcgaggcca gtcgctggac gatgacgctg
     1801 tcgggattga cccgccccct gaagcggccg gccaggtcca gcacataggc catgccgccg
     1861 gtcatgccgg cgccgaagtt gccgccggtg gggcccagga tcacggccgc gccgccggtc
     1921 atgtattcgc agccgttggc gccgcagccc tcgaccaccg tgacggcgcc ggaattgcgc
     1981 accgcgaacc gctcgcccgc ctggccggcg gcgaacagct tcccggccgt cgcgccgtag
     2041 aggcaggtgt tgccgatgat ggcgttggac tccggcgaag ccaggtgtga cgacggccgc
     2101 acgacgatgg tcgcgcccga cagacccttg ccgacgtagt cgttcgcctc gccggtcagc
     2161 acgatacgca ggccgcgcac ggcgaaggcg ccgagactct ggccggccga gcccttgagc
     2221 tcgagggtca gatggtccga cggcagggcg ctcatgccga acttgcgggt gatgtggctg
     2281 gagatgcgcg cgccgatcgc ccgggcggtg ttccgcacgg tgtaggtcag ctgcatcttc
     2341 tcgccgcgct ggagcagggg ggccgcgtcc cggacgatct gggcgtcgag cgtgtcgggc
     2401 acctcgttgc ggccctccac cgtgcagtag ggcttgttcg agcccgggtc ggcgcgaacc
     2461 agcagcgggt tgaggtcgag gtcgtcgaga tgttcgcccc cgcgggagac ctgggtcagc
     2521 aggtcggtgc ggccgacgat ctcctggatc gagcggaagc ccagccgggc gaggatggcg
     2581 cgcacctcct cggcgatgaa ggtgaacagg ttgaccacct tgtccggcgt tccggtgaag
     2641 cgcgcgcgaa gggcctcgtc ctgggtgcag acccccaccg ggcaggtgtt ggaatggcac
     2701 tgacggacca tgatgcagcc catagcgatc aggctggccg tgccgatgcc gaactcctcg
     2761 gcccccagca tggcggcgat gaccacgtcg cgaccggtgc ggatgccacc gtccgtccgc
     2821 aggcggacgg agtggcgcag gttgttgagg gtcagcacct ggttggcttc ggacaggccc
     2881 atctcccagg gcgagccggc gtatttgatc gaggtctgcg ggctggcgcc cgtgccgccc
     2941 acatggccgg agatcaggat cacgtcggcc ttggcctttg ccacgcccgc cgcgatggcg
     3001 ccgatgccgg tcatcgacac cagcttgacg gtgacccggg cgtccggatt gatctgcttg
     3061 agatcataga tgagctgggc gagatcctcg atcgagtaga tgtcgtgatg cggcggcggg
     3121 ctgatcagca tcaccccggg cgtggcgtgg cgcagccggg cgatcatctc ggtgaccttg
     3181 aagccaggca gctggccgcc ctcgccgggc ttggccccct gggcgacctt gatctcgatc
     3241 tcgcgacatt cgttgaggta ttcggcggtg acgccgaacc ggccggaggc cacctgcttg
     3301 accgcgctgt tgggattgtc gccgttgggc agcgggcgat agcgggcgcg atcctcgccg
     3361 ccctcgccgc tgaccgactt ggccccgatg cggttcatgg cgatgttcag cgcgccgtgc
     3421 gcctccgggc ccagggcgcc caggctcatg cccggggtca ggaaccgctt gcggatctcg
     3481 ttgacgctct cgacctcgtc cgggttcagc ggcttgcggt cgctgcgcca gtcgagcaga
     3541 tcgcgcagct ggatctgcgg cagggtgcgc atgccctcgg agaagcgccg gtaggtctcg
     3601 tagtcgccgg tgtcgcaggc gcgctgcagg gtgtggatca tgcgcgcctc gaaggcgtgc
     3661 gcctcgcccg agcggcgggc cttgtagaag ccgccgacag gcagggcgat gaaggccggg
     3721 tcccaggcgc gccggtgcag ctcgacggcg cgcttttcca ggcccgcgaa gccgatgccg
     3781 gaaatgcgcg agggcatgcc ggggaagaat tcggccacca gcgatcgcga caggccgacg
     3841 gcctcgaagt tgtagccgcc gcggtaggac gagatcaccg cgatgcccat cttggagatg
     3901 atcttcagca ggccctgctc gatggcggtc ttgaagttga cgcaggtgtc ccgcagcgtc
     3961 gcctcgccca gcaggccgcg gtcgagccgg tcctggaaac tctcctggct gagccaggcg
     4021 ttgaccgccg tggcgccgac cccgaccagc accgcgaaat aatgggtgtc catgcactcg
     4081 gccgagcgca cgatgatcga gacgtaggac cgcaggccct tgccaaccag atgggcgtga
     4141 accccgccgg tggccaggat catcggcagg gcgacccgct cggccgtgct gtgttcgtcg
     4201 gtcaggacga tggtggcgca gccgcgcagg gccgcgtcct cggcctcggc gcggatgcgg
     4261 tcgagattgg cgcgcagggc gtcgcccggc cgggcctcgt ccgccgggat cggcatggtg
     4321 cagtcgatca ccgccagcgc cttgtcgccg atgtagccca gcagccgctc gtacatgccg
     4381 gtggtcagca ccgggctctc gagcacatag acgtcggtct gggcctcgtc ctcgacaagg
     4441 atgttgccga ggttcttgaa gcgggtcttc aggctcatca ccccggtctc gcggagcggg
     4501 tcgatcggcg ggttggtcac ctggctgaag ttctgccgga aatagtggct cagcggacgg
     4561 tagttgtccg acagcacggc cagcggcgtg tcgtcgccca tcgagccgat cgcctccttg
     4621 ccgtcctcga ccatcggggc gagcaccagc tcgagatcct ccagcgtcat accggcggcg
     4681 gcctgacggc gggccagttc ctcgccggcg aaccgccgcg gctccgggcc ggggccgatg
     4741 cggctttcca gctcgaccat gttgccgagc cagtcggtgt agggatgggc gccggccagg
     4801 cgatcgatga tcgcctcctc gtcgtagagg cggccctcga ccaggtcgat ggcgatcatc
     4861 cgtccgggcg agatgttcag tttgcggacg atgcgggtct cctcgacgcc gcacatgccg
     4921 gcctcggagc ccatgatcac caggccgtcg tcggtatagg ccacacgcag cgggcgcagg
     4981 ccgttgcggt ccttgccggc cacgacccag cggccgtcgg tggcgcagag ggccgccggg
     5041 ccgtcccacg gctccatcac ggcgttgcag taggagtaga gcgcccggtg ctcgggcttc
     5101 atctgctcgt ctgaccgggt ggcccaggct tccggaatca gcagggcctt ggccatcggg
     5161 gccgaccggc cggcgcggac gagaatctcg taggtgttgt cgagcgccgc cgagtccgag
     5221 ccgctgggct ggatcaccgg cttcacgtcg tcgccgaatt ccccgaacgc ctgggcggcc
     5281 atgcggatct catggctctt catccagttg gtgttgccct tcagcgtgtt gatctcgccg
     5341 ttgtgggcca gcatccggaa cggctgggcc agccgccact gggggaaggt gttggtcgag
     5401 tagcgctggt ggaagatggc caccgccgcg gcgaaccgct cgtcgcgcag gtccggatag
     5461 aagtcgtcga tgttctcggc caggaacatg cccttgtaga tcagcgaccg ggccgacaac
     5521 gagcagatgt agaagtcgtt gatcgccgcg gcggcgacct ttttctcgat ccgcttgcgg
     5581 cacaggaaca gggcgcgctc cagcgcctcg ccttccatgc ccgacggcgg cgagagcatg
     5641 atctgctcga tctcgggccg ggtggcgttg gccttctcgc cgatcaccga ggtgtcgacc
     5701 ggcacctgac gccagccgta gatgtagaag ccgaagcgca gcgcctcgct ctcgacgatg
     5761 gtgcgggcgg cctcctgggc gccgaggtcc gtgcgcggca ggaagacctg gcccacggcg
     5821 atcgcccccg ggcgcagctt gtggccggtg ttgcgcacct ggtcggcgaa gaagtcctgc
     5881 ggcaccgaca gcaggatgcc ggccccgtcg ccggtcttgc cgtcggcgtc gacggcgccc
     5941 cggtgccaga cggccttgag cgccttgatg gcgaggtcga ccacctcgcg gcgcggcttg
     6001 ccgtcgatgg cgcagaccag gccgacgccg cagttgtcgc gctccgacga cggatcgtag
     6061 gcgtgaccat cgatcagccg ctggcggttc ttgagataga ggtcgagggc gtcgctcatg
     6121 tcggtcactc ggccgcgacg agggcgggag agtccgcctt cgcccgcagg tagctgtgga
     6181 tggaatcggc ggcgtcgcgg ccgtcgcgga tggcccagac gaccagcgag gcgccgcggg
     6241 cgatgtcgcc ggccgcgaac accccgtcga ggttggccgc ctgggtcctg cggtcgatct
     6301 tcaccgtgcc ccagcggctg atcgccaggt cgggctgacc gagggtggcc ggcaggtctt
     6361 ccgggtcaaa gccgagggcc ttgatcacca gccgggcggg caggtcgaag tccgaaccgt
     6421 cgaccggctc gggggcccgt cggcccgaac tgtcgggcgg gcccagccgc atgcggatcg
     6481 cgcgcacgcc cgtgacgccg tcggcgtcgc ccagcaggcc gcgcggcgcg gccagccatt
     6541 cgaagaccac gccttcctcc tcggcgttgg tcacctcccg catcgagccg ggcatgttct
     6601 cgcggtcgcg gcgatagagg caggtcaccg accgggcgcc ctgacgcacg gccgtgcgga
     6661 cgcagtccat cgccgtgtcg ccgccgccga caacgaccac gtccttgtcg gcggcgttga
     6721 gggtccccgc ctcgaagtcg gcgaccatgt cgcccatgcc cttgcggttg gaggcgatca
     6781 gatagtcgag cgcggcgacc acgccgtccg agccggcgcc cggcatttcc agttcgcggg
     6841 ccgcgtagac gcctgtcgcc agcagcaccg cgtcatgccg ttcgcgcaat tgcggcaggg
     6901 t
//
LOCUS       DAORNX010000117         6880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8164_length_6880_cov_7.982711, whole genome shotgun sequence.
ACCESSION   DAORNX010000117 DAORNX010000000
VERSION     DAORNX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_6880_cov_7.982711"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLO65_06695"
     CDS             complement(<1..483)
                     /locus_tag="PLO65_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27971.1"
                     /translation="MSYRSAGAAAAMALILAPVAAQATDFRVMGEDERAISVIEAAVK
                     TDSEGHRETGFFIGFSEPVGGPGGTQVIASTLLFDCPAGRYKVGLSRSFTAEMAPVQQ
                     TDGHYGWRTLVADSPFARAAAFACKGEALPKADGASVKAVVGAYLGRRAEAEAAAATA
                     G"
     gene            complement(548..1000)
                     /locus_tag="PLO65_06700"
     CDS             complement(548..1000)
                     /locus_tag="PLO65_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRD27972.1"
                     /translation="MSLEGAYDDGNIFAKIVRGEMPSVKVWEDDQVLAFMDVFPQARG
                     HVLVISKTSKARNLLDAEAKTIGRLIGAVQKTARAVKAALRPDGLVVTQFNGAPAGQT
                     VFHLHFHIIPRWDGVPLGRHGEGGMADMAELRLLADQIAAALDGPERS"
     gene            1058..1651
                     /locus_tag="PLO65_06705"
     CDS             1058..1651
                     /locus_tag="PLO65_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HRD27973.1"
                     /translation="MGAGSNGPGAEHLTDRQKKWFASVQASLERDTGKPLAEWVAIAR
                     TCPETTPRKRVEWLREHHGLGVNRIAHIPSEAFPPEAGWDSPEQLRANLWKDPASTAI
                     FEAVEAAVAGFPGLVTGQRKGFSAWSRNFQFAAVRPVKGGKARLGLCVTPGVDPRLVE
                     PKNEGWSERLKAAVTLDSPADVDGPLTALLRDAFDRS"
     gene            complement(1955..3106)
                     /locus_tag="PLO65_06710"
     CDS             complement(1955..3106)
                     /locus_tag="PLO65_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD27974.1"
                     /translation="MSLTPAVAIHRRISEIGRAAWDACAGADNPFVAFDFLDALEQSG
                     SVGDRTGWGPYHLTVADDAGQVAAAMPLYLKTHSQGEYVFDWSWADAYEKAGGRYYPK
                     LQCASPFSPVTGPRLLVRPDVDADEGRRMLLGGALTLCDKLGASSLHVTFPNETDWTF
                     LGNAGLLQRQDQQYHWFNRDYADFDAFLADLSSGRRKTVRRERRLAQEAVDEIVALTG
                     ADLTEDHWDAFYGFYMDTGGRKWGRPYLNRAFFSLLGERMADRVLLIMARRGGRWVAG
                     ALNLIGGDCLIGRNWGCVEDIPFLHFELCYYQAIEHAIRLGLPRVEAGAQGQHKIARG
                     YLPAAVYSAHWIADPALREPVRRYLDQERAAIRQEMDWLAEEYSPFKAV"
     gene            complement(3103..3876)
                     /locus_tag="PLO65_06715"
     CDS             complement(3103..3876)
                     /locus_tag="PLO65_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRD27975.1"
                     /translation="MKMKSRPEPGAFGEAWELLFSPPVAHRGLWTPGGAPENSLAAFQ
                     AACVAGYGIELDVQLTADGEAVVFHDYSLDRMTGEEGRLADRALHDLRDLRLAGTDET
                     IPTLAEVLVEVGHRALVHIELKTKFGEVGPLEKRVSEVLLDHHGPTCVIGFNPYSHAW
                     FADHHPQILRGLDSYDWKDGAASKLTPEIRQAYANLEHVELARPDFLALDAKMLPSPR
                     ADLFRARGMPIIAWTVRSAEEWERLGDHCDNLIFEGFEA"
     gene            complement(3873..4337)
                     /locus_tag="PLO65_06720"
     CDS             complement(3873..4337)
                     /locus_tag="PLO65_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD27976.1"
                     /translation="MSQVSHRLAAAGIVLPDPVAPIANYVPFVRAGDLVHISGQVSLD
                     AAGGITGIVGVDVDMATAVRAARLCGVNLLAQMRAACDGDLDRVRRVVKLGGFVQAGP
                     DFYDIPAVINGCSDVMVEAFGEAGRHARSAVGVYRLPRNFSVEVDAVVEIAR"
     gene            complement(4484..4921)
                     /gene="phaP"
                     /locus_tag="PLO65_06725"
     CDS             complement(4484..4921)
                     /gene="phaP"
                     /locus_tag="PLO65_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164441.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HRD27977.1"
                     /translation="MATGAETLKTTVEQFATAGNQAFKDNVEKSLATLNEVNAYSKKN
                     LEAVVASVTAATKGAEVLGAQAMAFSKKNIEDQVAAAKALAGAKSVQEAVELQTAWMK
                     TSLEGYMAEFAKWGETVSASVKDSVKPLNERVTATVEKLQAAK"
     gene            5205..6542
                     /locus_tag="PLO65_06730"
     CDS             5205..6542
                     /locus_tag="PLO65_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRD27978.1"
                     /translation="MFELARRLASAFALIAALATGLGAPTLATAQVPYLSIPASEPKY
                     AAIVVDANTGEVLFAKRADSPRYPASITKVMTLYLAFEAISEGRLKPTDLITVSAKAA
                     SQPPTKLGLAAGDKITVDDAMRAIAVKSANDMAVALAEHMGGTEQRFAALMTLRGQEL
                     GMTNTRYVNASGLPDSRQISTARDIAILSRAVMRDYPQYYSYFSLRSFTYRGVTMTNH
                     NRLMGQFWGMDGLKTGYTNASGFNLAASAVRDGKRLIAVVLGGRSTATRDDNVEDLLM
                     TGFDVLKRRSLGETITIAQTMFEPVVEGPIERPSYEQGDADQEGLRVVLTDGPASTTP
                     YVVQSRMAPVPDPVTPARKAAATPKKAAPPRKPRGQWAIQAGAFKSKSQARQQLSLIQ
                     RRFASAVHEGEAAIGNAVGGYFRVRFEGMTADAARAACKTLKAKKQACMVIAP"
     gene            complement(6630..>6880)
                     /locus_tag="PLO65_06735"
     CDS             complement(6630..>6880)
                     /locus_tag="PLO65_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRD27979.1"
                     /translation="VALAAAGLAMAVTARRGDLPTPKMDQADLAEAREILGVAANATR
                     EEIQAAYARLIRAVHPDAGGTSGLAARLNAARDRLLKK"
BASE COUNT         1065 a         2391 c         2346 g         1078 t
ORIGIN      
        1 cccggcggtt gccgccgccg cctcggcctc ggcccggcgg ccaaggtagg cgccgacgac
       61 cgccttgacg ctggccccgt cggcctttgg cagcgcctcg cccttgcagg cgaaggcggc
      121 ggcccgggcg aacggcgaat cggccaccag ggtccgccag ccgtagtgac catcggtctg
      181 ctgcaccggc gccatctccg ccgtgaagct gcgcgagagg ccgaccttgt agcgaccggc
      241 cgggcagtcg aagagcaggg tcgaggcgat cacctgggtc ccgccggggc cgccgacggg
      301 ctcggagaag ccgatgaaga agccggtctc gcggtggccc tcggagtcgg tcttgaccgc
      361 agcctcgatc acgctgatcg cccgctcatc ttcgcccatc acccggaagt cggtcgcctg
      421 ggccgcgacg ggcgccagga tcaacgccat cgcggcggcg gctcctgcgg atcggtagct
      481 catcggtcat ctccctggtc gagaacggct ggcccgtcgt cggtcctgtg tcgagactgg
      541 agccggatca tgacctttcc gggccatcca gcgcggcggc gatctggtcg gccaggagcc
      601 tgagctcggc catgtcagcc atgcccccct ccccgtgccg gccgagcggc acgccgtccc
      661 accgggggat tatgtggaaa tgcagatgga acaccgtctg gccggccggc gcgccattga
      721 actgcgtcac caccagcccg tcgggcctga gcgccgcctt cacggcccgc gcggtcttct
      781 gcaccgcccc gatcaggcgg ccgatggttt tggcttcggc gtcgagcagg ttgcgggcct
      841 tcgaggtctt cgagatcacc agcacatgcc ccctggcctg gggaaacacg tccatgaagg
      901 ccagcacctg gtcatcctcc cagaccttca cgctgggcat ttctccccgc acgatcttcg
      961 cgaagatgtt gccgtcgtcg taggcgccct cgaggctcat tgatgcgctc cctgcaggag
     1021 gtaatgtcct atcaaaggca gcgcggagcg caatcgcatg ggcgcgggaa gcaacggacc
     1081 gggcgcggaa catctcaccg accgccagaa gaagtggttc gcctcggtcc aggccagtct
     1141 ggagcgcgac acgggcaagc ccttggccga atgggtggcg atcgcgcgga cctgccccga
     1201 aaccacgccc cgcaaacgcg tcgagtggct gcgcgagcac catggcctgg gcgtcaaccg
     1261 tatcgcccac atcccgtcgg aggcgttccc gccggaagcg gggtgggact ctccggaaca
     1321 gctgcgcgcc aatctctgga aggatcccgc ctcgacggcc atcttcgagg cggtcgaggc
     1381 ggcggtggcg ggctttcccg gcctggtcac tgggcagcgc aaggggttct ccgcctggtc
     1441 gcggaacttc cagttcgccg ccgtgcgccc ggtcaagggc ggcaaggcgc ggctgggcct
     1501 gtgcgtgacg cccggcgtcg acccgcgtct ggtcgagccg aagaacgagg gctggtccga
     1561 gcggctgaag gcggcggtca ccctggactc gcctgcggat gtggacgggc cgctgacggc
     1621 gctgctcagg gacgcgttcg acaggtcctg agagctgggg ggcaggcttt cctgtccctt
     1681 gggactggaa ggcctgcccc ggtcccgctg cggcgccggg acagggcaag ccgtcccgag
     1741 ggacagccag ggccaatcga cagtcagcgc gcgggagccg ccaggggctc cgattgttcc
     1801 cgcggaagcg ggaacccaag cggtgtttcg ggtcctgaca cgctgtttcg gcggccaaac
     1861 gcgcttgggt ccccgccttc gcggggacag tcggtgagaa aacggcccca ggtcgtgagt
     1921 gtggcctggt cctcgtccag tccccgcgcc tcccctacac cgccttgaac ggcgaatact
     1981 cctccgcgag ccagtccatt tcctgccgga tcgccgcccg ttcctggtcg aggtagcggc
     2041 ggaccggctc gcgcagggcc gggtcggcga tccagtgggc ggaatagaca gccgccggca
     2101 gatagccgcg ggcgatcttg tgctggccct gcgccccggc ctcgacgcgc ggcaggccca
     2161 ggcggatcgc gtgttcgatg gcctggtagt agcagagctc gaaatgcagg aacgggatgt
     2221 cctcgacaca gccccagttg cggccgatca ggcagtcacc gccgatcagg ttcagggccc
     2281 cggccaccca gcgcccgccg cgccgggcca tgatcagcag cacccggtcg gccattcgct
     2341 cgccgagcag cgagaagaag gcgcggttca ggtagggccg gccccatttg cggccgccgg
     2401 tgtccatgta gaagccgtag aaggcgtccc agtggtcctc ggtcaggtcc gctccggtca
     2461 gggcgacgat ctcgtccacg gcctcctgcg ccagacgacg ctcgcgcctg acggtcttgc
     2521 gccggcccga cgacaggtcg gcgaggaagg cgtcgaagtc ggcgtagtcg cggttgaacc
     2581 agtggtactg ctgatcctgc cgctgcagca gcccggcgtt gccgagaaac gtccagtccg
     2641 tctcgttcgg aaaggtcacg tgcaggcttg aggcgccgag cttgtcgcag agggtcaggg
     2701 cgccgccgag caacatccgc cgcccctcgt ccgcatcgac gtcgggccgg accagcagcc
     2761 gggggccggt caccggcgag aacggcgagg cgcactggag cttgggataa tagcggccgc
     2821 cggccttctc gtaggcgtcg gcccacgacc agtcgaagac gtattcgccc tggctgtggg
     2881 tcttcagata gagcggcatg gcggccgcca cctggccggc gtcgtcggcc acggtgaggt
     2941 gatagggacc ccagcccgtg cggtcgccga ccgaaccgga ctgctccagc gcatcgagga
     3001 agtcgaaggc gacgaacggg ttatccgcac ccgcgcaggc gtcccaggcc gcacggccga
     3061 tctcgctgat gcggcggtga atggcgaccg ccggcgtcag gctcacgcct cgaagccctc
     3121 gaagatcaga ttatcgcaat ggtcgccgag ccgctcccac tcctcggccg aacggaccgt
     3181 ccaggcgatg atcggcatgc cccttgcgcg gaacaggtct gcgcgcggcg agggcagcat
     3241 cttcgcgtcc agcgccagga agtcgggccg ggcgagctcg acatgttcga gattggcgta
     3301 ggcctggcgg atctccggcg tcagcttgct ggccgccccg tccttccagt cgtagctgtc
     3361 gagcccgcgc aggatctgtg gatggtgatc ggcgaaccag gcgtggctgt aggggttgaa
     3421 accgatcacg caggtcggcc cgtggtgatc cagcagcacc tcgctgaccc gcttctccag
     3481 cggcccgacc tcgccgaact tcgtcttcag ctcgatgtgc acgagcgcgc ggtggccgac
     3541 ctcgaccagc acctcggcga gggtcggaat ggtctcgtcg gtcccggcca ggcgcaggtc
     3601 gcgcagatcg tgcagcgcgc ggtcggcgag ccgcccctcc tcgcccgtca tgcggtccag
     3661 gctgtagtcg tggaacacca ccgcctcacc gtcggccgtc agctgcacgt ccagctcgat
     3721 gccgtagccc gcgacgcagg cggcctggaa ggcggccagc gaattctcgg gcgcgccgcc
     3781 cggcgtccac aggccccgat gcgccacggg cgggctgaac agcagttccc aggcctcgcc
     3841 aaaggcgccc ggctcgggtc tggacttcat cttcatcggg cgatctccac cacggcgtcg
     3901 acctcgaccg agaagttgcg cggcaggcga tagacgccga cggccgagcg ggcatggcgg
     3961 ccggcttcgc cgaaggcctc gaccatcacg tcggagcagc cgttgatcac ggcggggatg
     4021 tcgtagaagt ccggtccggc ctggacgaag ccgcccagct tgaccactcg ccggacccgg
     4081 tcgaggtcgc cgtcgcaggc cgcgcgcatc tgcgccagca ggttgacgcc gcacaggcgg
     4141 gcggcgcgga cggcggtcgc catgtcgaca tcgacgccga cgattccggt gatgccgccg
     4201 gcagcgtcga gcgacacctg gccggaaata tggaccagat cccctgccct cacgaacggc
     4261 acgtagttgg cgatcggggc gacggggtcg ggcaggacga tgcccgcggc ggcaaggcga
     4321 tgggagactt gagacatggc gcgaagctag cgcgcccggg gcggcggcgg aagcgatccc
     4381 cggacgtaaa aaggcccggg tcgtcgccga cccgggcctt cacgcgaact tgaaccccgg
     4441 agggccttac tggggggagc tcgcccgccc ggcgtccagg ttcttacttc gcggcctgca
     4501 gcttctcgac ggtcgcggtg acgcgctcgt tcagcggctt gaccgagtcc ttgacggagg
     4561 cggaaacggt ttcgccccac ttggcgaact cggccatgta gccttccagc gaggtcttca
     4621 tccaggcggt ctgcagctcg acggcttcct gcacgctctt ggcgccggcc agggccttgg
     4681 cggccgcgac ctggtcttcg atgttcttct tggagaaggc catcgcctgg gcgcccagga
     4741 cttccgcgcc cttggtggcg gcggtgaccg aagcgacgac ggcttcgagg ttcttcttgg
     4801 aataggcgtt gacttcgttg agcgtcgcca gcgacttctc gacgttgtcc ttgaaggcct
     4861 ggttaccagc ggtggcgaac tgctcgaccg tggtcttgag ggtttcggcg ccagtggcca
     4921 tcgggagtct cctgtgtggt cggggaggac cgggatttgg gttcggcggc gactcaggtc
     4981 gctgctgatg ctccccatgt ctcatgttgc agtgcagcag tcaatgaatt tttgtgcggt
     5041 gcaacatgac cgtttcgcga cgcacaacgc cgggacttcc cctgcggcgg attcgctcaa
     5101 caggtgttta ctttggcgaa agacggaaca atgcatgcta gctgaatgcc gcagcgcagc
     5161 ttcggagccg ctctgtaaac cgtgactttt tcgacggacg ccgcatgttc gagctcgccc
     5221 gccgcctcgc ttccgcattc gccctgatcg ccgccctggc gaccggactc ggcgcgccga
     5281 cgctggccac cgcccaggtg ccttatctga gcattccggc ctcggagccg aaatacgcgg
     5341 ccatcgtggt ggacgccaac accggcgagg tgttgttcgc caagcgcgcc gacagcccgc
     5401 gctatccggc ctcgatcacc aaggtgatga ccctctacct ggccttcgag gcgatctctg
     5461 aaggccggct caagccgacc gacctgatca ccgtctcggc caaggccgcc tcgcagcccc
     5521 cgaccaagtt gggcctggcc gcgggcgaca agatcaccgt cgatgacgcc atgcgggcca
     5581 ttgcggtgaa gtcggccaac gacatggccg tcgccctggc cgagcatatg ggcggcaccg
     5641 agcagcgctt cgccgccctg atgaccctgc ggggccagga actcggcatg accaacacgc
     5701 ggtacgtcaa cgcctcgggc ctgcccgaca gccgacagat ctcgaccgcc cgcgacatcg
     5761 ccatcctgtc gcgcgcggtg atgcgcgact acccgcagta ctacagctac ttcagcctgc
     5821 gctcgttcac ctaccgcggg gtgaccatga ccaaccacaa ccggctgatg ggccagttct
     5881 ggggcatgga cgggctgaag accggctaca ccaacgcctc gggcttcaac ctggcggctt
     5941 cggcggtgcg cgacggcaag cgcctgatcg ccgtggtgct cggcggccgc tcgaccgcga
     6001 cccgcgacga caatgtcgaa gacctgctga tgaccggatt cgatgtgctc aagcgccggt
     6061 cgctcggcga aaccatcacc atcgcccaga ccatgttcga accggtggtc gagggcccga
     6121 tcgagcggcc gtcctacgag cagggcgacg ccgaccagga aggcctgcgg gtggtcctga
     6181 ccgacggccc cgcctccacc accccctatg tcgtgcagag ccgcatggcg ccggtgcccg
     6241 atcccgtgac tccggcccgc aaggcggcgg cgacgccgaa gaaggccgcc ccgcccagga
     6301 agccgcgcgg ccagtgggcc atccaggccg gggccttcaa gtccaagtca caggcccgtc
     6361 agcagctgag cctgatccag cgccggttcg cctcggccgt gcacgagggc gaggcggcca
     6421 tcggcaacgc cgtaggcggc tacttccgcg tccgtttcga aggcatgacc gccgacgccg
     6481 cccgcgccgc ctgcaagacc ctcaaggcca agaagcaggc ctgcatggtc atcgcgccct
     6541 gatcctcaag gggacatgta ccctaggtac gtgtccccga cttcctgacc ggtgggtttc
     6601 ggggacacgt acctgcggta catgtcccct tatttcttca gcaatcgatc ccgcgccgcg
     6661 ttgagcctgg ccgccaggcc gctcgtgccg cctgcgtcgg ggtgcacggc gcggatcagc
     6721 cgggcatagg cggcctggat ctcctcgcgc gtggcgttcg cggcgacgcc gagaatttcg
     6781 cgcgcctcgg ccaggtcggc ctggtccatc ttcggggtcg gcaggtcgcc gcgacgcgcc
     6841 gtgaccgcca tcgccaggcc ggcggcggcc agcgccacac
//
LOCUS       DAORNX010000118         6880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8165_length_6880_cov_6.427692, whole genome shotgun sequence.
ACCESSION   DAORNX010000118 DAORNX010000000
VERSION     DAORNX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8165_length_6880_cov_6.427692"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLO65_06740"
     CDS             <1..557
                     /locus_tag="PLO65_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27980.1"
                     /translation="IGPAFDAVEAALGPISILVNNAGVGGDGMAMDMSVETFDQTFAV
                     NVRGVYFGAVEAARRMIASGVAEAGEARIINIASIAAFNQLPGLSVYCASKAACASLT
                     KGLAREWARPRIAVNAICPGYIETEINADWFRTEGGQKQIKSFPRRRLMDEDVLDEAL
                     LMLAGRRAHFITGTLITIDDGQTL"
     gene            611..877
                     /locus_tag="PLO65_06745"
     CDS             611..877
                     /locus_tag="PLO65_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27981.1"
                     /translation="MADTKLTLQLDDKRFAALRKAAEEAGESVETFAERLIVRGLKHD
                     DWAISEARIENYERTGVAEDAETVFTHVREHLSRRLAERSAPVK"
     gene            1215..2342
                     /locus_tag="PLO65_06750"
     CDS             1215..2342
                     /locus_tag="PLO65_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD27982.1"
                     /translation="MGKGPLSGLKIVEFAGIGPGPFCGMLLSDLGADVVRVDRKGAGR
                     ASPADVTSRGRRSVALDLKSPDAIEAVLKLLESADGLIEGFRPGVMERLGLGPDVVLK
                     RNPKLVFGRMTGWGQTGPYAKAAGHDMNYIAITGALHAIGTSDKPVPPLNLVGDFGGG
                     ALYLAFGLMAGIISARETGKGQVIDCAMSDGAASLMAMFYGFKGAGIWKDERRSNLLD
                     GGAHFYDTYQCADGKWISIGSIEPQFYALLLEKTGITDPQFANQMSRDEWPELKAKLA
                     GVLATKTQAEWCAIMDATDICFAPVLTLDEAPRHPHNVARETFVTVDGVVQPAPAPRF
                     SATPGAIQGPPPKIGAHNDEALAQWGFSTAEIEALKASGAL"
     gene            2565..3497
                     /locus_tag="PLO65_06755"
     CDS             2565..3497
                     /locus_tag="PLO65_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRD27983.1"
                     /translation="MSTAKRPDPTVHLDLDPVHWARATIEVLGKALQRLWGRDVMLYV
                     GGVSFWIMLAVFPLLVIAVGLYGLLLTPEQVAHQAEVLARMLPSDARGMFQDELQRLA
                     HAPSAAVSTQSVIALFIGGYAAHRGYKALLAGLSFIHDEENQRGFVSFNVMALLVLIA
                     SLVLVGLVSVLFLGLRVASAAWNLRPLAGLNWLYSEWTWTSICMLLAMSLIYRFAMSR
                     EPVAWRASIAGGVASAVLALVASWLCAIYVEQIAHLGATYGSVGTVVVFLIWLSWNVN
                     ALFFGGALATEMEIALHTRKIKPVLKRRKRAPPR"
     gene            complement(3454..3741)
                     /locus_tag="PLO65_06760"
     CDS             complement(3454..3741)
                     /locus_tag="PLO65_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRD27984.1"
                     /translation="MTTSDANGPPLNAAPAGEVANEVLVDARGHRCPVPTLRLRRALE
                     TAGPGGVVRLLADDPMARIDVPHFANETGYTVVDRGDLTAGGLFFVVSRPA"
     gene            3740..4582
                     /locus_tag="PLO65_06765"
     CDS             3740..4582
                     /locus_tag="PLO65_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRD27985.1"
                     /translation="MAPRPTWQGHLRLSLVTCPVALYTATSPGGDVHFNLLHKDTHNR
                     IRMIPTDPELGPVERSDLVKGFEYEKDHYIVVTPDEIDAVRLESTRTIDIERFVDRDD
                     IDRLYWDSPYFLAPDGKLAVEAYTVIREAMRRTGKIALGRLVMHQRERLIAIEPQERG
                     LVAWSLRSRDEVRDADDVFGDIPAHKADAGMIAIAEKIIAQQEGPFDPSEFNDRYEDA
                     LRALIEEKQKGKGHKVTAAEPEDTNVVDLMDALRRSLGKKGATPARKAAPAKAKAAAR
                     KKAS"
     gene            complement(4626..>6880)
                     /gene="ligD"
                     /locus_tag="PLO65_06770"
     CDS             complement(4626..>6880)
                     /gene="ligD"
                     /locus_tag="PLO65_06770"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HRD27986.1"
                     /translation="KKGELKVVFAGERMQGGYVLVRIRNDRTGGKRSNWLLIKHRDDF
                     AREGDKDRLLRDTAFSVASGRTMEDIAAGRGKAPRPFITRVRRGAGAVWNGKGSPEAS
                     GEAEAARHDSPRAAKAARPKTAAALPGFVEPQLCKLVDRPPRGAGWAHEIKFDGYRMQ
                     LRVEGGAAALKTRKGLDWTAKFPEIAADAAGLSDSLVDGEIVALDADGSPDFAGLQAA
                     LSDGKTQDLVFFAFDLLFAAGEDLRALPLSERKARLQTLLDKGGGARIRYVEHFATGG
                     DAVLSSACRMSLEGIVSKRLSAPYRSGRGDTWRKSKCRAGHEVVIGGWTSNGGAFRSL
                     IAGVMRDGKLVHVGRIGTGFGRDKVARLLPRLKALETDRSPFAARLPRGHGDTHWVKP
                     RLVAEIEYAGFTGDGAIRQAAFKGLREDKPASEVEAETPAPAKTRLAAPQPTGAAGNV
                     VMGVTLSSPDKVLWPDAGDDRPVTKLDLARYYEAVADWMMPHIARRPCSTIRTPEGIE
                     GERFFQRHLGKGASALLSEVALAGDHKPYLVIERKEALAALAQTSTTELHPSNCRPDL
                     PEVPGRLVFDLDPDEGLGFADVIVAAKEVRERLEALGLVAFCKTTGGKGLHVVTPLAP
                     PRRGRLDWPAAKAFAHEVCLRMAADSPDRYVVNMSKKLRKGRIFLDYLRNDRLSTAVA
                     PLSPRARPGGTVSMPLTWTQVRTGLEPKRFTIRTAPAMVAKMTAWEDYADSERSLEDA
                     IKRLGGKRLV"
BASE COUNT         1078 a         2362 c         2369 g         1071 t
ORIGIN      
        1 ccatcggtcc ggcgttcgac gcggtggaag cggcgctggg gccgatctcg atcctggtca
       61 acaacgccgg cgtcggcggc gacggcatgg ccatggacat gagcgtcgag accttcgacc
      121 agaccttcgc ggtcaacgtg cggggcgtct atttcggggc cgtggaggcg gccaggcgga
      181 tgatcgccag cggcgtggcc gaggccggcg aggcgcggat catcaatatc gcctcgatcg
      241 ccgccttcaa ccagctgccg gggctgagcg tctactgcgc ctccaaggcc gcctgcgcct
      301 cgctgaccaa ggggctggcg cgcgagtggg cgcggccgcg catcgcggtc aacgccatct
      361 gccccggcta catcgagacc gagatcaatg ccgactggtt caggaccgag ggcggccaga
      421 agcagatcaa gagcttcccc cgtcgccggc tgatggacga ggacgtgctg gacgaggccc
      481 tgctgatgct ggccggccgc cgcgcgcact tcatcaccgg gacgctgatc accatcgacg
      541 acgggcagac gctgtaggtg caattatgag gagacgtttg ggcgccgctg caatctgatg
      601 gtgtatgtgg atggctgaca ccaagcttac gctgcaactc gacgacaagc gcttcgccgc
      661 cttgcgaaaa gcggccgagg aagcgggcga gtcggtcgag actttcgcgg agaggctgat
      721 cgtacgcggt ctcaagcacg atgactgggc gatctcggaa gcccggatcg aaaactacga
      781 gcgcacgggt gtcgctgagg acgcagagac ggtcttcacc catgtgcgcg agcatctcag
      841 ccggcggctg gctgaaaggt cggcgccggt caagtgaggc gacgggttcg cttcctgtcg
      901 agcgcccaca gagatttggt gcgtctcgcc gactttctgg cgggcgacga tcaggcgaga
      961 gccatggagg ccatggatgt cattctcgcc gggccgcgag tctggcggag tatgccgagc
     1021 gcggcgcacc ggtagagcca ccgagcgggc ggaagctcgt cgcgcccttc ggcaaggcgg
     1081 cctacatcat ccataacctc gtccatcccg acgaggtgat cgtcacccgc atcttccacc
     1141 gtcttgaaga ccggccgctt gcctgaccgt gcgtcagtgg ctttagctct cgcccaaacc
     1201 agaggaaaca caccatgggc aagggtccgc tttcgggtct gaagatcgtc gagttcgccg
     1261 gcatcgggcc gggaccgttc tgcggcatgc tgctgtcgga cctgggcgcc gatgtggtgc
     1321 gggtcgaccg caaaggcgcg ggccgtgcct cgccggccga cgtgaccagc cgcggccgcc
     1381 gctcggtggc gctggacctg aagtcgcccg atgcgatcga ggcggtgctc aagctgcttg
     1441 aaagcgccga cggcctgatc gagggcttcc gtcccggcgt catggagcga ctgggcctgg
     1501 gcccggacgt ggtgctcaag cgcaatccga agctggtgtt cggccgcatg accggctggg
     1561 gccagaccgg accctacgcc aaggccgccg gccacgacat gaactacatc gccatcaccg
     1621 gggcgctgca cgccatcggc acgagcgaca agcccgtgcc gccgctgaac ctggtcggcg
     1681 actttggcgg cggggcgctg tacctcgcgt tcggcctgat ggcggggatc atcagcgcgc
     1741 gcgagaccgg caagggccag gtcatcgact gcgccatgag cgacggcgcc gcctcgctga
     1801 tggccatgtt ctacggcttc aagggcgcgg gcatctggaa ggatgagcgg cgatcgaacc
     1861 tgctcgacgg cggcgcccac ttctacgaca cctaccagtg cgccgacggc aaatggatct
     1921 cgatcggctc gatcgagccg cagttctacg cgcttctgct tgaaaagacc ggcattaccg
     1981 atccgcagtt cgccaatcag atgagccgtg atgagtggcc ggaactgaag gccaagctgg
     2041 ccggtgtgct ggcgaccaag acccaggccg aatggtgcgc gatcatggac gccaccgaca
     2101 tctgcttcgc gcccgtgctg acgctcgacg aggccccgcg ccacccgcac aacgtggccc
     2161 gcgagacctt cgtcaccgtg gacggcgtgg ttcagccggc ccccgcgccg cgcttttcgg
     2221 ccacgccggg cgcgatccag gggccgccgc caaagatcgg ggcccataac gacgaggcgc
     2281 tcgcccagtg gggcttctcg accgccgaga tcgaggcgct gaaggcgtcg ggggcgctgt
     2341 agggtcacgg cgaacaacgc aggctgagaa cgctgttcag ccacggttca gccaggcgat
     2401 tccattctcg acctgtggcc ggctctcgcg ctctcccgtc cctcgaagcg agggtcggct
     2461 gcacaggacg aaggcaaggt catcctgtca ggcgcgtcgc ggtcaaacag ccgcggcgcg
     2521 cctttgacgt tgcgccggac ggctggcgcg ggcaggttcc gccgatgtcg accgccaagc
     2581 gtcccgaccc cacggtccac ctcgatctcg accctgtgca ttgggcgcgc gcgaccatcg
     2641 aagtgttggg caaggccctg cagcgactct ggggccgcga cgtgatgctc tatgtcggcg
     2701 gcgtgtcctt ctggatcatg ctggcggtgt ttccgctgct ggtgatagcc gtgggcctct
     2761 acggcctgct gctgacaccc gaacaggtgg ctcaccaggc ggaggtgctc gcgcgcatgc
     2821 tgccgtccga cgcgcggggc atgttccagg acgaactgca gcgactcgcc cacgccccgt
     2881 cggcggcggt gtcgacccag agcgtcatcg ccctgttcat cggcggctac gcggcccatc
     2941 gcggctacaa ggcgctgctc gccgggctgt cgttcatcca tgacgaggag aaccagcgtg
     3001 gcttcgtcag cttcaacgtg atggcgctgc tggtgctgat cgcctcgctg gtgctggtcg
     3061 ggctggtgtc ggtgctgttc ctcggcctgc gcgtggcgtc tgcggcctgg aacctcaggc
     3121 cgctggcggg gctgaactgg ctctacagcg aatggacctg gaccagcatc tgcatgctgc
     3181 tggccatgag cctgatctac cggttcgcca tgtcgcggga gccggtggcc tggcgggcgt
     3241 cgatcgccgg cggagtcgcc tcggcggtgc tggcgctggt cgcctcgtgg ctctgcgcca
     3301 tctacgtcga gcaaatcgcc cacctgggcg cgacctatgg ctccgtcggc acggtggtgg
     3361 tgttcctgat ctggctgagc tggaacgtca acgcgctgtt cttcggcgga gcgctggcga
     3421 ccgaaatgga aatcgccctg catacgcgga agatcaagcc ggtcttgaaa cgacgaaaaa
     3481 gagccccccc gcggtaagat cgccgcggtc cacgaccgtg taaccggtct cgttggcgaa
     3541 gtgcggcacg tcgatgcgcg ccatcgggtc gtccgccagc agccgcacga ccccgcccgg
     3601 cccggccgtc tcgagcgccc gcctcagccg cagggtcggc accgggcagc gatggccgcg
     3661 cgcgtccacc agcacctcgt tcgcaacctc gcccgcgggc gccgcgttca gcgggggtcc
     3721 attcgcatcg gaggtcgtca tggcgccgcg tccaacctgg cagggtcacc tgcgcctgtc
     3781 gctcgtgacc tgtccggtgg ctctctacac cgccaccagc cccgggggcg acgtgcattt
     3841 caacctgctg cacaaggaca cccacaaccg catccgcatg atcccgaccg atccggagct
     3901 cggcccggtc gagcggtcgg acctggtcaa gggcttcgaa tacgagaagg accactacat
     3961 cgtcgtcacc cccgacgaga tcgacgcggt ccggctggag agcacgcgga ccatcgatat
     4021 cgagcggttc gtcgatcgcg acgacatcga ccggctctac tgggacagtc cctatttcct
     4081 cgcgccggac ggcaagctgg ccgtcgaggc ctacaccgtc atccgcgaag ccatgcgcag
     4141 gaccggcaag atcgcgctcg gccggctggt gatgcaccag cgggagcggc tgatcgccat
     4201 cgagccgcag gaacggggac tggtcgcctg gagtctgcgc agccgcgacg aggtgcgcga
     4261 cgcggacgac gttttcggtg acattccggc gcacaaggcc gacgcgggca tgatcgccat
     4321 cgccgagaag atcatcgccc agcaggaagg gccgtttgat ccatccgaat tcaacgaccg
     4381 ctacgaggac gccctgcgcg cgctgatcga ggagaagcag aagggcaagg gccacaaggt
     4441 gactgccgcc gaacccgagg acaccaatgt ggtcgacctg atggacgccc tgcgccggag
     4501 cctgggcaag aagggcgcga cgcccgcgag gaaggccgcg cccgccaagg cgaaggcggc
     4561 cgcacgaaag aaggcctcct gatcctcgac acgcatctcc cgcgagggag aggcgtgtgg
     4621 tgaggctaga ccaaccgctt gccgccgagc cgcttgatgg cgtcctccag cgaccgctcg
     4681 ctgtccgcgt agtcctccca ggccgtcatc ttcgccacca tcgccggcgc cgtgcggatc
     4741 gtgaacctct tcggctccag acccgtccgc acctgggtcc aggtcagcgg catcgacacc
     4801 gtcccgcccg gccgcgcgcg gggcgacagg ggcgcgaccg ccgtgctgag ccggtcgttg
     4861 cgcaggtaat caaggaaaat ccgccccttg cgcagctttt ttgacatgtt cacgacgtag
     4921 cggtccgggc tgtcggccgc catccgcagg cagacctcgt gggcgaacgc cttggcggcg
     4981 ggccagtcca gccggccgcg gcggggcggg gcaaggggcg tgaccacatg gagccccttg
     5041 ccgccggtgg tcttgcagaa ggcgaccagg cccagcgcct cgagccgctc gcggacctcc
     5101 ttggccgcga cgatgacgtc ggcgaagccc agcccctcgt cgggatccag gtcgaacacc
     5161 agacggcccg ggacttcggg caggtcagga cggcagttgg agggatgcag ttcggtggtc
     5221 gaggtctggg ccagggcggc aagggcctcc ttccgttcga tgaccagata gggcttgtgg
     5281 tcaccggcca gggccacctc gctgagcagg gccgaggccc ccttgcccag gtgccgctgg
     5341 aagaaccgct cgccctcgat cccctccggc gtgcggatgg tcgaacaggg ccggcgggcg
     5401 atgtgcggca tcatccagtc ggccaccgcc tcgtagtagc gggcgagatc cagcttggtc
     5461 accggccggt cgtcgccggc gtcgggccag agcaccttgt ccggactgga cagggtgacc
     5521 cccatcacga cgttgccggc ggcgcccgtc ggctggggtg cggcgagcct ggtcttcgcc
     5581 ggagccggcg tctccgcctc gacctccgag gccggcttgt cctcgcgcag ccccttgaag
     5641 gcggcctggc ggatcgcgcc gtcgccggtg aagcccgcgt actcgatctc ggccaccagc
     5701 cgcggcttga cccagtgggt gtcgccatgg ccccgcggca gccgcgccgc gaacggcgag
     5761 cggtcggtct ccagcgcctt cagccgcggc agcagccggg cgaccttgtc gcggccgaag
     5821 cccgtgccga tccggccgac gtgcaccagc ttgccgtcgc gcatgacccc ggcgatcagc
     5881 gatcgaaagg ccccgccgtt gctggtccag ccgccgatga ccacctcgtg accagcgcgg
     5941 cacttggact tgcgccaggt gtcgccgcgg ccggaccggt agggggcgct gagcctcttt
     6001 gagacgatcc cctcgagcga catacggcag gcggaactca gcaccgcgtc gccgcctgtc
     6061 gcgaaatgct cgacatagcg gatgcgtgcg ccgcccccct tgtccagcag cgtctgaagg
     6121 cgcgccttgc gctcggagag gggcagggcc cgcaggtcct cgccggccgc gaacagcagg
     6181 tcgaaggcga agaacacgag gtcttgcgtc ttgccgtccg acagggccgc ctgaaggccg
     6241 gcgaagtcgg gcgagccgtc ggcgtcgagc gccacgatct cgccgtcgac caggctgtcg
     6301 gacaggccgg ccgcgtccgc ggcgatctcg gggaacttcg ccgtccagtc caggcccttg
     6361 cgggtcttca gcgccgccgc gccgccctcg acgcgcagct gcatgcgata gccgtcgaac
     6421 ttgatctcat gggcccagcc cgcgccccgc ggcggacgat cgaccagctt gcacagctga
     6481 ggctcgacga aaccgggcag ggcggcggcc gtcttcggcc gggcggcctt cgcggccctt
     6541 ggcgagtcgt gccgcgcggc ctcggcctcg ccgctggctt caggcgagcc cttgccgttc
     6601 cagacagcgc ccgcgccccg ccggaccctg gtgataaacg gcctgggcgc cttgcccctg
     6661 ccggccgcga tgtcctccat cgtgcggccc gaggcgaccg agaaggcggt gtccctgagg
     6721 agccggtcct tgtcgccctc gcgggcgaag tcgtcgcggt gtttgatcaa cagccagttg
     6781 gagcgctttc cgccggtcct gtcgttgcgg atgcgcacca gcacgtagcc gccctgcatg
     6841 cgctcgccgg cgaagacgac cttcagctca cccttcttca
//
LOCUS       DAORNX010000119         6809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8283_length_6809_cov_8.682114, whole genome shotgun sequence.
ACCESSION   DAORNX010000119 DAORNX010000000
VERSION     DAORNX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8283_length_6809_cov_8.682114"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PLO65_06775"
     CDS             complement(<1..154)
                     /locus_tag="PLO65_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD27987.1"
                     /translation="MISGLVVEDLAIRRGERLLFERLSFEARAGEAVALVGRNGAGKT
                     SLLRAVA"
     gene            356..3052
                     /gene="acnA"
                     /locus_tag="PLO65_06780"
     CDS             356..3052
                     /gene="acnA"
                     /locus_tag="PLO65_06780"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRD27988.1"
                     /translation="MASIDSLQTRRTLTVGGTSYVYYSLSAAEDAGLKGVSRLPVSMK
                     VLLENLLRNENGGDTSEADLKAVAAWVDNKGGVEHEISFRPARVLMQDFTGVPAVVDL
                     AAMRDAMNALGADPTKINPLIPVDLVIDHSVMVDFFGTAKSYGENVDREYERNIERYR
                     FLRWGSSAFNNFRVVPPGTGICHQVNLEYLAQTVWTAPDAGLEHEVAYPDTVVGTDSH
                     TTMVNGLSVLGWGVGGIEAEAAMLGQAIPMLIPEVIGFRLTGALPEGATATDLVLTVT
                     QMLRKKGVVGKFVEFFGSGLAHLTIEDQATIANMAPEYGATCGFFPISQSTIDYLRAT
                     NRDAQRVNLVEAYAKAQGLWFDPDAEEPVFSDVLELDLGQVQSSLAGPKRPQDRVLLN
                     DAAAAFETALADEFGKKDQTDRRVAVAGESFDVGHGDVVIAAITSCTNTSNPSVLIAA
                     GLLAKNAVAKGLTVKPWVKTSLAPGSQVVTDYLAKAGLTKHLDALGFNLVGYGCTTCI
                     GNSGPLPEAISAAVNEGDLVACSVLSGNRNFEGRVNPDVRANYLASPPLVVAYALAGS
                     MRIDLATQPLGKDKKGNDVFLKDIWPTSKDIADLQRKSVTSEMFAKRYGDVFKGDKHW
                     QAIKVEGGQTYAWDIGSTYVKNPPYFEGMSMTPAGVKDIIEARILGVFGDSITTDHIS
                     PAGSIKKTSPAGEYLLQHQVRFEDFNGYGSRRGNHEVMMRGTFANIRIRNRITPDIEG
                     GVTRHFPSGQVMPIYDAAMRYEAEGRPAVVFAGKEYGTGSSRDWAAKGAKLLGVRAVI
                     AETFERIHRSNLVQMGVLPLQFLSDGWQRLGLTGEEIVSIRGLQDLSPRKQLIVELYR
                     PSDGRIARFPVRCRIDGPTELEYFQNGGVLNYVLRNLAGGAA"
     gene            3203..3898
                     /locus_tag="PLO65_06785"
     CDS             3203..3898
                     /locus_tag="PLO65_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HRD27989.1"
                     /translation="MRKALFALVLLLLFPTASPALARPPVVVELFTAQGCSSCGAANA
                     LVGELAERPGVLALTFGVDYWDYLGWPDTFARPEFTERQRAYARRLALREVYTPQVVV
                     DGRAQASGVKDDAVEDLVAAALKTPSWPPDMMFMGERRVAVGSGPAPRGGADVWLVRY
                     DPRALEVTPRRGDNKGQTLVQRNVVREMVRLGAWRGRPTAYRIPSTTLEGLAMAVLVQ
                     APKGGKILAVLAK"
     gene            complement(4010..4744)
                     /locus_tag="PLO65_06790"
     CDS             complement(4010..4744)
                     /locus_tag="PLO65_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD27990.1"
                     /translation="MGRLEGRRAIVTGAASGIGRASAELFASEGARVLAVDRPGANLA
                     FEHPAIESLGRDISEDDAPAAIVSRAVDAFGGLDIVYNNAGVSGSTPVGETSDESFDR
                     QIAVNLRAAFRISREAVPHLVRSGVGRLIFTASIMARHTDNGLVAYSASKAGVVGMMR
                     TFALELGRHGVTANAILPGAIATGMTAASFAHDEIAALWAKKAALKRLGQPIDIARAA
                     LFLASDDGGFVTGQALGVDGGLMLRV"
     gene            5079..5414
                     /locus_tag="PLO65_06795"
     CDS             5079..5414
                     /locus_tag="PLO65_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HRD27991.1"
                     /translation="MAFDPTYSQPAAAVVFFERRELERLLRLYGRMVAAGEWRDYAIA
                     GLSESCVFSVFRRASEQPLYRIEKRPALARKQGQWAVLTQSGHILKRGHDLEQVLRVF
                     DKGRFTVVE"
     gene            complement(5446..5802)
                     /locus_tag="PLO65_06800"
     CDS             complement(5446..5802)
                     /locus_tag="PLO65_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27992.1"
                     /translation="MAESDDDRPRHTAADYYGFFLEELLEIIADGEAAGMAPAATDLL
                     RRAYGRFRIEFEQRYPSAGFKAPLEPPAASDSAAGDPVPWHVVAAIVDDAFASEADVD
                     AIELLNQAERLLRPRV"
     gene            complement(5922..6674)
                     /locus_tag="PLO65_06805"
     CDS             complement(5922..6674)
                     /locus_tag="PLO65_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRD27993.1"
                     /translation="MSDFERGTARSIPQAADMTMDAGLRAFMLGVYNKMALGLLLSAV
                     LAYATSSIPAVTSLLYVTTPTGALAGFTLLGQIVRFAPLAMVLVVMLGMRNMTARSSG
                     IYYWSIVTAIGAGLGIWLLIYTGASVFMTFLITATAFGALSLVGYTTKKDLSGFGSFL
                     IMGVWGLVIASLVNMFLHLPAIYWIVNVVGVLIFAGLTAYDTQRLKLTYYQVKGSGEG
                     MAVATNMGALSLYLDFINLFQFLLAFMGGRRS"
BASE COUNT         1128 a         2334 c         2305 g         1042 t
ORIGIN      
        1 cggccaccgc ccgcagcagg ctggtcttgc ccgcgccgtt gcggccgacc agggccacgg
       61 cctcgccggc ccgggcttcg aacgacaggc gctcgaagag cagccgctcg ccgcggcgta
      121 tggcgaggtc ctcgacgacc agtccggaaa tcatcgcggc gcctcgcgga ccgccgaaaa
      181 gtccctgacg cgggcatgga cgtatggcgc gaccgggcgt atgcagcgcg ccatcggccc
      241 cccgcgtgcg cgcggggaga tgcgccgcgc cgggggggac gctgtgagtc ctttccgagc
      301 gcgcgatccc ggaagtggtt ttcgggcggc cgagaacagg aaaggactcc cccacatggc
      361 gtctatcgat agcctgcaaa cccgccgcac cctgacggtc ggcggaacct cctacgtcta
      421 ctacagcctg agcgcggcgg aagacgccgg cctgaaaggc gtgagccggc tgcccgtgtc
      481 aatgaaggtt ctgctcgaga acctgctgcg caacgaaaac ggcggcgaca ccagcgaagc
      541 cgacctcaag gccgtggcgg cctgggtcga caacaagggc ggcgtcgaac acgagatcag
      601 cttccgcccg gcccgcgtgc tgatgcagga cttcaccggc gtgccggccg tcgtcgacct
      661 ggcggccatg cgcgacgcca tgaacgccct gggcgccgac ccgaccaaga tcaacccgct
      721 gatccccgtc gacctggtca tcgaccactc ggtgatggtc gacttcttcg gcacggccaa
      781 gagctatggc gagaacgtcg accgcgagta cgagcgcaac atcgagcgct atcgcttcct
      841 gcgctggggc tcttcggcgt tcaacaattt ccgcgtggtg cccccgggca ccggcatctg
      901 ccaccaggtg aacctcgaat acctggccca gaccgtctgg accgcgccgg acgccggcct
      961 cgaacatgag gtcgcctatc ccgacaccgt ggtcggcacc gactcccaca cgaccatggt
     1021 caacggcctg tcggtgctgg gctggggcgt cggcggcatc gaggccgagg cggccatgct
     1081 cggccaggcc attccgatgc tgatcccgga agtgatcggc ttccgcctga ccggcgccct
     1141 gccggaaggc gccaccgcca ccgacctggt gctgaccgtc acccagatgc tgcgcaagaa
     1201 gggcgtggtc ggcaagttcg tcgagttctt cggctcgggc ctggcccacc tgaccatcga
     1261 ggatcaggcg accatcgcca acatggcccc cgagtacggc gccacctgcg gcttcttccc
     1321 gatcagccag tcgaccatcg actacctgcg cgccaccaac cgcgacgccc agcgggtcaa
     1381 tctggtcgag gcctacgcca aggcccaggg cctgtggttc gatccggacg ccgaggaacc
     1441 ggtgttcagc gacgtgctcg agctcgacct ggggcaggtg cagtcctcgc tggctggtcc
     1501 gaagcggccg caggatcgcg tgctgctgaa tgacgccgcc gcggccttcg agaccgcgct
     1561 ggccgacgag ttcggcaaga aggaccagac cgaccgccgc gtcgcggtgg ccggcgagtc
     1621 cttcgacgtg ggccacggcg acgtggtcat cgccgccatc accagctgca ccaacacctc
     1681 caaccccagt gtcctgatcg ccgccggcct gctggccaag aacgcggtgg ccaagggcct
     1741 gaccgtcaag ccgtgggtca agacctcgct ggcccccggc tcgcaggtgg tcaccgacta
     1801 tctggccaag gccggcctga ccaagcacct cgacgccctg ggcttcaacc tggtcggcta
     1861 tggctgcacc acctgcatcg gcaactccgg cccgctgccg gaagcgatca gcgccgcggt
     1921 caacgagggc gacctggtcg cctgctcggt gctgtcgggc aaccgcaact tcgagggccg
     1981 ggtgaacccc gacgtgcggg ccaactacct ggccagcccg ccgctggtgg tggcctacgc
     2041 cctggccggt tcgatgcgca tcgacctggc gacccagccg ctcggcaagg acaagaaggg
     2101 caacgacgtc ttcctgaagg acatctggcc gacctcgaag gacatcgccg acctccagcg
     2161 caagtcggtc accagcgaga tgttcgccaa gcgctacggc gacgtgttca agggcgacaa
     2221 gcactggcag gcgatcaagg tcgagggcgg ccagacctac gcctgggaca tcggctcgac
     2281 ctatgtgaag aacccaccct atttcgaggg catgtcgatg acgccggccg gcgtgaagga
     2341 catcatcgag gcgcgcatcc tcggcgtctt cggcgactcc atcaccaccg accacatcag
     2401 cccggccgga tcgatcaaga agacctcgcc ggccggcgag tacctgctgc agcaccaggt
     2461 ccggttcgag gacttcaacg gctacggcag ccgccgcggc aaccacgaag tgatgatgcg
     2521 cgggaccttc gccaacatcc gcatccgcaa caggatcacg ccggacatcg agggcggggt
     2581 gaccaggcat ttcccgtccg gacaggtcat gccgatctat gacgccgcca tgcgctacga
     2641 agccgagggc cggcctgccg tggtcttcgc cggcaaggaa tacggcaccg gcagttcgcg
     2701 cgactgggcg gccaagggcg ccaagctgct cggcgtgcgc gccgtcatcg ccgagacgtt
     2761 cgagcgcatc caccgctcga acctcgtgca gatgggcgtg ctgccgctgc agttcctgtc
     2821 ggacggctgg cagcgcctgg gcctgaccgg cgaggagatc gtctcgatca ggggcctgca
     2881 ggacctgtcg ccgcgcaagc agctgatcgt cgagctctac cgcccgtcgg acggccgcat
     2941 cgcccgcttc ccggtgcgct gccgcatcga tggcccgacc gagctggagt atttccagaa
     3001 cggcggtgtg ctgaactacg tgctgcgcaa cctcgccggc ggcgcggcct gagcgacagg
     3061 ggacatgtac cgcaggtacg tgtcccctaa atcctgaaca cggtcttggg ggacacgtac
     3121 cggcttcgcc ggaacatgtc cccttcccgt tcacggcctc gtgaaacgcg tctccggtcc
     3181 ttctccgatt aagtgcggcg cgatgcgcaa agcccttttc gccctcgtcc tgctgctgtt
     3241 gtttccgacg gcgtcgccgg cgcttgccag gccgccggtg gtggttgaac tgttcaccgc
     3301 ccagggctgc tcgtcctgcg gcgcggccaa cgccctggtc ggcgagctcg cagagcggcc
     3361 cggcgtcctg gccctgacct tcggcgtcga ctactgggac tatctgggct ggcccgacac
     3421 cttcgcccgc cccgagttca ccgagcgcca gcgcgcctac gcccgcaggc tggcgctgcg
     3481 cgaggtctac acgccgcagg tggttgtcga cggccgggcg caagcgtccg gagtcaagga
     3541 cgacgcggtc gaggatctgg tggccgcggc cctgaagacg ccgtcctggc cgccggacat
     3601 gatgttcatg ggcgaacgcc gggtggcggt cggctcgggg cctgcgccgc gtggcggggc
     3661 cgacgtctgg ctggtccgct acgatccccg cgcgctggaa gtgacgccgc gtcgcggcga
     3721 taacaagggc cagaccctgg tccagcgcaa tgtcgtgcgc gagatggttc gactgggcgc
     3781 ctggcgcggc cggccgacgg cctaccggat tccgtcgacc accctggagg ggctggccat
     3841 ggccgtgctg gtccaggcgc cgaagggcgg gaagatcctc gccgtgctgg cgaagtagca
     3901 gccctgcgtc cttcgacacg gcccttcggg cctgctcagg atgacgtggc ggggttgtcg
     3961 ctgaaccacg tcatggtgag cagcgcgtag cgcgtgtcga accacgcctt cacacccgca
     4021 gcatcagccc gccgtcgacg ccgagggcct ggcccgtcac gaagccgccg tcgtcggacg
     4081 ccaggaacag cgccgccctc gcgatgtcga tcggctggcc caggcgcttg agcgccgcct
     4141 tcttggccca cagggcggcg atctcgtcat gggcgaaact ggcggcggtc atgcccgtgg
     4201 cgatggcccc gggcaggatg gcgttggccg tcacgccatg gcggcccagt tccagcgcga
     4261 aggtgcgcat catgcccacg accccggcct tggacgccga ataggccacc aggccattgt
     4321 cggtgtgccg cgccatgatc gaggcggtga agatcagccg cccgacgccc gacctgacca
     4381 ggtgcggcac ggcctcgcgg ctgatgcgga aggcggcccg caggttgacg gcgatctgcc
     4441 ggtcgaagga ctcgtcgctg gtctcgccaa ccggcgtcga gccggacacg ccggcgttgt
     4501 tgtagacgat gtcgaggccg ccgaaggcgt ccaccgcccg gctgacgatg gcggccggcg
     4561 cgtcgtcctc gctgatgtcc cgaccaaggc tctcgatggc ggggtgctcg aaggcgaggt
     4621 tggcgccggg ccggtcgacg gccagcaccc gggccccctc gctggcgaag agctcggcgc
     4681 tggcccggcc gatgccgctg gcggcgcccg tgacgatggc ccggcggcct tcgagtctgc
     4741 ccatggtcgt ttccgcccct gtcctgtgct gcggacaaaa tgaaaccccc gctgccggag
     4801 gggaaggcaa cgggggttca taggaggggc gttcaggtat cggccggccc gaaacgcgcg
     4861 atcccagggg ggctgggggg gctgttcagg atccgggacc agcgccgctt ccgaccggcc
     4921 gacgctgtta aactgggggc cgttaccggc gggcgcgcgt ccgaaataag gtcttttgga
     4981 ggcggttggt aaccggcggt ccacaatccc ggcgaccgtg gccgatccgt gacgcccctc
     5041 gccaaaccgc tcgattcagg tctagcctgc ggaccggcat ggcgttcgat ccgacatact
     5101 cccagcccgc cgccgcggtg gtctttttcg agcggcggga actggaacgg ctgctgcgac
     5161 tgtacggacg aatggtcgcc gcgggcgagt ggcgcgacta cgccatcgcg ggcctgtcgg
     5221 agagctgcgt cttcagcgtt ttccgccgcg cgtcggaaca gccgctctac cgcatcgaga
     5281 agcgcccggc cctggcccgc aagcagggcc agtgggcggt gctgacccag tcgggacaca
     5341 tcctcaagcg cggccacgac ctggagcagg tcctgcgggt cttcgacaag ggccggttca
     5401 ccgtcgtgga ataggcgttg cgggaaggtc gagcttggtc ccgagctaaa ctcgaggccg
     5461 gagcaaccgt tcggcttgat tcaacagctc aatggcgtcg acgtcggctt ctgatgcaaa
     5521 tgcgtcatcg acgatggccg ccacgacatg ccagggcact gggtcgccag ccgcgctgtc
     5581 acttgccgcc ggcggttcca gcggagcctt gaaacccgcg gacgggtatc gctgctcgaa
     5641 ctctatgcga aagcggccgt aggcgcgccg caacaggtcg gtggcggccg gcgccattcc
     5701 ggcagcctcc ccgtcggcga tgatctccaa caactcctcc agaaaaaagc cgtagtagtc
     5761 ggcagccgtg tggcgtggcc gatcatcatc gctctctgcc atgggcccgc gctgcctgag
     5821 ggttcgtgag accgcaatat cgcttcaagc gagcaaagct ccaaggaaaa ggccccgcca
     5881 gtttcccgac ggggcctgat cgtttgagcg ctgcgtcgcg gctagctgcg acggccgccc
     5941 atgaaggcga gcaggaactg gaacaggttg atgaagtcca gatacaggct cagcgcaccc
     6001 atgttggtgg ccacggccat cccctcgccg ctgcccttga cctggtagta ggtcagcttc
     6061 agccgctggg tgtcgtaggc ggtcaggccc gcgaagatca gcacaccgac cacgttgacg
     6121 atccagtaga tcgccggcag gtgcaggaac atgttgacca ggctggcgat gaccaggccc
     6181 cagacgccca tgatcaggaa gctgccgaag cccgacaggt ccttcttggt cgtgtagccg
     6241 accaggctca gggcgccgaa ggcggtggcc gtgatcagga aggtcatgaa caccgaggcg
     6301 ccggtgtaga tcagcagcca gatgcccagg cccgcgccga tcgccgtgac gatcgaccag
     6361 tagtagatgc ccgagctgcg cgcggtcatg ttgcgcatgc cgagcatgac caccagcacc
     6421 atggccagcg gcgcgaagcg cacgatctgt ccgagcaggg tgaagccggc cagggcgccg
     6481 gtcggggtgg tgacatacag caggcttgtc accgccggga tgctggaggt cgcgtaggcc
     6541 agcacggccg acagcagcag gcccagcgcc atcttgttgt aaacgccgag catgaaggcg
     6601 cggagacccg cgtccatcgt catgtcggcg gcctgcggga tcgagcgcgc cgtaccgcgt
     6661 tcgaagtcgc tcatggaatg aacccttttc atcgcgtccg agatggacgc gcatcgaata
     6721 tcgggtgtgc ggcagccggg cgcaagcggt gctcacgcct atgttttcaa ggctactccg
     6781 cgcgcagcac cggcgcgggg cgcttcgac
//
LOCUS       DAORNX010000120         6792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8313_length_6792_cov_6.599822, whole genome shotgun sequence.
ACCESSION   DAORNX010000120 DAORNX010000000
VERSION     DAORNX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_6792_cov_6.599822"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLO65_06810"
     CDS             complement(<1..201)
                     /locus_tag="PLO65_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD27994.1"
                     /translation="MNRLHRWLPLGAAMAMMIGLTGARSASWSDLDRDYVAFIHRQDP
                     LRAGGRGDREALTRWPDNAPAAI"
     gene            389..859
                     /locus_tag="PLO65_06815"
     CDS             389..859
                     /locus_tag="PLO65_06815"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRD27995.1"
                     /translation="MRLALFQPVIPQNVGACIRLSACFGVALHVIEPVGFRFDDAGMK
                     RAALDYGPLGHMTRHADWDAFQQDRSPGRLVLFTTRGATALDAFAFHPDDVLLFGSET
                     AGAPPAVHDAAGARVVIPLKPGARSLNLSVSAGIGLFEALRQTGGLSRHAGPGT"
     gene            complement(935..1612)
                     /locus_tag="PLO65_06820"
     CDS             complement(935..1612)
                     /locus_tag="PLO65_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRD27996.1"
                     /translation="MTFDEVVLGRRSIRGYKPEPVPRALIREVLELAMRAPSSLNTQP
                     WNFYVLAGDALDRIRAGNTERNLAGVPSSREFRSHGEYAGVHRERQIEIAKQLFAAMG
                     IERDNKEKRTDWVLRGFRQFDAPVSIVVTYDRSIHGGDIAPFDCGAVTNALVNAAWSR
                     GLGCVVNSQGIMQSPVVREHAGIPDDQVIMICVAMGWPEDSFPANAVVSRRKSVDEAA
                     VFVGFDD"
     gene            complement(1700..2605)
                     /locus_tag="PLO65_06825"
     CDS             complement(1700..2605)
                     /locus_tag="PLO65_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD27997.1"
                     /translation="MSEAVGVTIVVPAFNAAAHLDETLDSLLAQDHPRLQIIVVDDGS
                     TDDTAAIAAARGDRLTLLRQSNRGQSAALAAGWAAAAHGIIGYLSADDRLAPGAVGRC
                     ASALQARPDVVLVYPDYHVIDAASCRRATVSRADYDRRLLFGDVRCLPGPGALFRRAA
                     YEAAGPWRSDYRHVPDMEFFLRLALLGDFLHLPEALADYRLHDGSITGRPVPRTWADE
                     PLRMVDAFYSRQDLPPEIRAWRGRARANAGLLAARYHTKSGRWPTALIRIAGSMPDCP
                     PVVISRLRPAGLVRFARRALDGRRA"
     gene            complement(2598..3719)
                     /locus_tag="PLO65_06830"
     CDS             complement(2598..3719)
                     /locus_tag="PLO65_06830"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HRD27998.1"
                     /translation="MMTIIGTRPEIIRLSRVIARFDAHVEHLLVHTGQNHDYELNEVF
                     FSELGLRAPDIFLAAAGGNPAETIGQVIIAIDRVFETHRPDAVLILGDTNSSLAAIAA
                     KRRKIPIFHMEAGNRCFDARVPEEINRRIVDHIADINLPYSAIAREYLLREGLPPDQI
                     IVTGSPLREVLEHYRPGIEASDVVARLGLTPGRYFLVSAHREETVDPPACLARLLAIL
                     NDLAERWGDPVIVSTHPRMRRRLAELGASPHPGIRFERPFGLFDYGSLQMNARAVLSD
                     SGTITEESAILNFPALNLRETHERPEGSEEASVMLVGLNLARILQGLAILETQPRGEG
                     RLLALPADYRPTNVSDKILRLVVGYTDFVNRKVWRRTDV"
     gene            complement(4031..4966)
                     /locus_tag="PLO65_06835"
     CDS             complement(4031..4966)
                     /locus_tag="PLO65_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD27999.1"
                     /translation="MSGLAAGATGLAVRVGRERFNMQTASPRRILVLGATGMLGQTTV
                     RLLAAAPGLAVTGTVRSDAACGRFPEELRKTLIRDVDVRDATTLERVFRDIRPEVVIN
                     AVGVIKQRPECDDPRVAMPVNAELPHRLARLCAATGGRLIHLSTDCVFGGARGDYVED
                     DEPDAIDLYGRSKLMGEVVDGDAITIRTSLIGHELREGLGLLEWFLAQRGEVTGFTKA
                     IFSGLPVVELACVLRDHVIPRPGLRGLFHVAGPPISKDALLRLLARVYDHPVEIIADD
                     RVVIDRSLNATRFNRLTGYRPPPWPTLLRRLRDFG"
     gene            5080..6039
                     /locus_tag="PLO65_06840"
     CDS             5080..6039
                     /locus_tag="PLO65_06840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD28000.1"
                     /translation="MSWKHRIVDLLIAVWLPLPARVLRFLRYRFRYFPLSKAVMIRAG
                     VFPVIDHYHDAFYDPATLRDLRAPRPLPAIDFNLAAQIALLEALPEAPELAALPEAPT
                     GDRGFHLANNMFEHGDAELWYQVIGHFRPRRIIEVGSGHSTKIARLAIDRAALTDPDY
                     RCDHVCIDPFAPDWLDSLGPRVLRQKVEATSLDLFRQLERDDILFIDSSHVIRPQGDV
                     LFQYLHLLPVLAPGVIVHIHDVFSPRDYPDTWLRDRVYFWNEQYLVEAFLSHNTEWEI
                     LLAGNMLMHEAYDLLKSRCLHLDRDREPRSLYIRRLSPRVPGA"
     gene            6036..>6792
                     /locus_tag="PLO65_06845"
     CDS             6036..>6792
                     /locus_tag="PLO65_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28001.1"
                     /translation="MTPPLSAWSAIGQVLGPGRRAGLAGLLALMVFGMLLELAGVGLV
                     IPVIQLLSGPVPRSEATLVGHWASLVGARDHAGLVRVTMLCLVIFFAFKLACQALVAL
                     RLAAFRYDLQADIARRLYALYLAQPFGFHVRRNSAHLISTVSNEAHDAAQATHHGLAL
                     IAELLVICGLAVLLIIASPLAGIAVASLVAVAAWFSLRLTRTGLNAWGVRLRHHQALK
                     MKSLQQGLGGIKLIKLGGLEAAALDHFDGHNLAS"
BASE COUNT         1043 a         2338 c         2339 g         1072 t
ORIGIN      
        1 gatggcggcc ggcgcgttgt ccggccagcg ggtcagcgcc tcgcggtcgc cgcggccgcc
       61 ggcccgcagc ggatcctgtc gatggatgaa ggcgacatag tcccgatcga gatccgacca
      121 gctcgccgac cgcgcgccgg tcaggccgat catcatggcc atggcggcgc ccaggggcag
      181 ccagcggtgg agacggttca tcgaacggat tccggcggcg ggagtcgcaa gctttgtccg
      241 gcggcccggt cggcgcaagg gcggtgtcag gcgagggccg tcgcttccgc gagcagattg
      301 gggctggcca agatcgtcgc cccggcggaa agacgtcggt ctccgaccgt tcatgacccc
      361 gcccctcagc atcatgcctc ccagccggtt gcgcctcgcc ctgttccagc ccgtgattcc
      421 gcagaatgtc ggcgcctgca tccggctgtc ggcctgtttc ggcgtcgccc tgcacgtgat
      481 cgagccggtc ggcttccggt tcgacgacgc cgggatgaag cgcgcggcgc tcgactacgg
      541 cccgctcggg cacatgaccc ggcacgccga ctgggacgcc ttccagcagg accgatcgcc
      601 gggccggctg gtgctgttca cgacccgcgg cgcgacagcg ctcgacgcct tcgccttcca
      661 tcccgacgac gttctgctgt tcggcagcga gacggccggc gcgccgccgg cggtgcacga
      721 cgcggcgggc gcccgtgtgg tcattccgct gaagccaggg gcccgatcac tgaacctgtc
      781 ggtcagcgcc ggcatcggcc tgttcgaggc cctgcgccag acgggcgggc tgtcccggca
      841 cgccgggccc gggacctagg tcaaatcgac attcagcgcg cgggagtcgc caagcggcga
      901 gaagatggcc ccacgtcgtg aatgtcgatt ggttctagtc gtcgaacccg acgaacaccg
      961 ccgcctcgtc gaccgacttg cggcgggaca ccacggcgtt ggccggaaag ctgtcctcgg
     1021 gccatcccat ggcgacgcag atcatgatca cctggtcgtc ggggatgccg gcatgctcgc
     1081 ggaccacggg cgactgcatg atcccctggc tgttgaccac gcagcccagg ccccgcgacc
     1141 aggccgcgtt gaccagggcg ttggtcaccg cgccgcagtc gaacggcgcg atatcaccgc
     1201 cgtggatcga ccggtcatag gtgacgacga tcgacacggg agcgtcgaac tgacgaaagc
     1261 cacgcagcac ccagtcggtc cgcttctcct tgttgtcccg ctcgatgccc atggccgcga
     1321 acagctgctt ggcgatctcg atctggcgct cccggtggac cccggcgtac tcgccgtggc
     1381 tgcggaactc gcgggacgac ggaacgccgg ccagattgcg ctcggtgttg ccggcgcgga
     1441 tgcggtcaag cgcgtcgccg gccagcacat agaagttcca gggctgcgtg ttgagcgacg
     1501 acggcgcgcg catcgccagt tcgagcacct cgcggatcag cgccctgggc acgggttcgg
     1561 gcttgtagcc gcggatgctt cggcgtccga ggacgacctc atcaaacgtc atggtcgatc
     1621 ctgcgcctga cttcatcccg agacccccgt tgctgtcttg acgccgtggt acagcacagg
     1681 cggctcggcc gtaaactcgt caggcgcgtc ggccgtccag ggcgcgacgc gcgaaccgga
     1741 cgaggccggc ggggcgcaac cgcgagatga cgaccggggg acagtccggc atggagcccg
     1801 cgatacggat cagggcggtg ggccagcggc ccgacttggt gtgatagcgc gccgccagca
     1861 ggccggcgtt ggcgcgcgcc cgacctcgcc aggcgcggat ctcgggcggc agatcctgcc
     1921 ggctgtagaa ggcgtcgacc atgcgcagcg gctcgtccgc ccaggtccgg ggcacgggcc
     1981 gaccggtgat cgagccgtcg tgcagacggt agtcggccag ggcctccggc aggtgcagga
     2041 aatcacccag cagggccagc cgcaggaaga actccatgtc gggcacatgg cggtagtcgg
     2101 accgccaggg ccccgccgcc tcataggctg cccggcggaa cagcgcgccg ggaccgggca
     2161 ggcagcgaac atcgccgaac agcagccggc ggtcatagtc ggcccgactc actgtcgccc
     2221 gccgacacga agccgcgtcg atgacgtggt agtctggata gacgagcacg acgtcgggcc
     2281 gggcctggag ggcagaggcg cagcgaccga ccgcgcccgg agccagccgg tcgtccgcag
     2341 aaaggtagcc gatgatgccg tgggccgccg cggcccaccc ggcggccagg gcggcgctct
     2401 ggccgcgatt cgactggcga agaagcgtca gccggtcgcc gcgggccgcg gcgatggcgg
     2461 cggtgtcgtc ggtcgatccg tcgtccacga cgatgatctg aaggcgcgga tgatcctgcg
     2521 ccagcaggct gtcgagcgtc tcgtccaggt gggcggcggc attgaacgcc gggacgacga
     2581 tcgtgacgcc gacggcctca gacatcggtg cggcgccaga ccttgcggtt gacgaagtcc
     2641 gtatagccga cgaccagccg gaggatcttg tccgagacat tggtcgggcg gtagtcggcc
     2701 ggcagcgcca gcagacggcc ctcgccgcgt ggctgcgtct cgaggatggc caacccctgc
     2761 aggatgcgcg cgaggttcag cccgaccagc atcaccgacg cctcctccga gccttccggc
     2821 cgctcgtgcg tttcccgcag gttgagcgcc gggaagttga gaatggcgct ttcctccgtg
     2881 atcgtcccgc tgtccgacag gacggcccgg gcgttcatct gcaggctgcc gtagtcgaac
     2941 aggccgaacg gtcgctcgaa ccggataccg ggatgagggc tcgcgccgag ctcggccagg
     3001 cggcggcgca tgcgcggatg ggtcgagacg atgaccgggt cgccccagcg ctcggcaagg
     3061 tcgttgagga tggcgagcag ccgcgccagg caggccggcg gatcgaccgt ttcctcgcga
     3121 tgggcgctga ccaggaaata gcggccgggc gtcaggccaa gccgggcgac gacgtccgaa
     3181 gcctcgatgc cgggccggta gtgctcgagc acctctcgca ggggacttcc ggtgacgatg
     3241 atctggtccg gcggcaggcc ctcccgcagc agatactccc gcgcgatggc gctgtagggc
     3301 aggttgatgt cggcgatatg gtcgacgatc cggcgattga tttcctccgg cacccgcgca
     3361 tcgaaacagc gattaccggc ctccatgtgg aagatcggga tcttgcggcg cttggcggcg
     3421 atggcggcca ggctgctgtt ggtatcgccg agaatcagca cggcgtcggg ccggtgcgtc
     3481 tcgaagaccc ggtcgatggc gatgatgacc tggccgatcg tctcggcggg attgccgccg
     3541 gccgccgcca gaaagatgtc gggcgcgcgc aggcccagtt ccgaaaagaa cacctcgttc
     3601 agctcgtagt cgtgattctg ccccgtatga acgagcaggt gttcgacgtg agcgtcgaac
     3661 ctggcgatca cccgggacag ccggatgatc tccggccggg tgccgataat cgtcatcacc
     3721 ttgagcaacg ggatctccac acgcaggcgc ggtcagcggg cgccgcccga tggcgacggc
     3781 ctggcgaggg gcattgtcgt atgccccgtg gcgcgggggg aagcgccaaa tcctcgacct
     3841 gggcggccag ggccaatcga cattcagcgc gcgggcgccg ccaagggctc cgattgttcc
     3901 cgcgaaggcg ggaacccaag cggtgtttcg ggtcctgacg cgccgtttcg gcggcgacct
     3961 gggcttgggt ccccgccttc gcggggacaa tcggtgagaa aacgccccag gtcgtgaatg
     4021 tcgattggct ctagccgaag tcgcgcaagc gccgcagcag ggtcggccac ggcggcgggc
     4081 ggtagcccgt taggcgattg aaacgggttg cattgagcga ccggtcgatg accacccggt
     4141 catcggcgat gatctcgacc gggtggtcat agacgcgggc cagcagccgc agcagggcgt
     4201 ccttgctgat cggtggcccc gcgacgtgaa acagcccgcg aaggccggga cggggaatca
     4261 catgatcgcg cagcacgcag gccagctcga ccaccggaag tcccgagaag atcgccttcg
     4321 tgaagccggt gacctcaccc cgctgcgcga ggaaccattc cagcagtccc agtccctcgc
     4381 gaagttcatg gccgatcagt gacgtgcgga tggtgatcgc gtcgccgtcg accacctcgc
     4441 ccatgagctt gctgcgcccg tagaggtcga tggcgtcggg ctcgtcatcc tcgacatagt
     4501 cgccgcgcgc cccgccgaag acgcagtccg tgctcaggtg aatcagccgc ccgcccgtcg
     4561 cggcgcagag ccgtgcaagc cgatgcggca gctcggcgtt gaccggcatg gccacccggg
     4621 gatcgtcgca ctcgggtcgc tgcttgatga cgcccaccgc gttgatcacc acctcgggcc
     4681 ggatgtcccg gaacacccgc tccagggtcg tggcgtcccg gacgtcaacg tcgcggatca
     4741 gagtctttcg gagctcttcc gggaaccggc cgcaggcggc gtcggaccgg accgtcccgg
     4801 tgacggccag gccgggagcg gcggcgagca gacggaccgt ggtctgcccc agcatgcccg
     4861 tggcgccgag gaccaggatc ctgcggggac tggccgtctg catgttgaac cgttccctcc
     4921 cgacccgaac ggccagcccc gtcgccccgg cggccagacc ggacatgcgc ggggtagccg
     4981 ccgcggcgac catgccttat tcccgaagcg tcggccaaat gtcgcgaccg ccgccgcgtc
     5041 gggcggccat caagcggttc tgggatctgc gctcaagcca tgagttggaa acacagaatc
     5101 gtcgacctgc tgatcgcggt ctggctgccg ctgccggcgc gggtgctgcg attcctgagg
     5161 taccgcttcc ggtatttccc tctcagcaag gccgtgatga tcagggccgg ggtgtttccg
     5221 gtgatcgatc actatcacga cgccttctac gatccggcga cactgcggga tctgcgggcg
     5281 ccgcggccgc tgccggcgat cgatttcaat ctcgccgccc agatcgccct gctggaggcc
     5341 ctgcccgaag cgcctgaact ggccgccctg ccggaggcgc cgacgggcga tcgtggattt
     5401 cacctggcca acaacatgtt cgagcacggc gacgccgagc tctggtacca ggtcatcggt
     5461 catttccggc cgcgccggat catcgaggtc gggtcgggcc actccaccaa gatcgcccgc
     5521 ctcgccattg accgggccgc gctgaccgac cccgactacc gttgcgacca cgtctgcatc
     5581 gatccgttcg cgccggactg gctggactcg ctggggcctc gcgtgcttcg ccagaaggtg
     5641 gaagcaactt ccctggacct gttccggcag ctggaacgcg acgacatcct gttcatcgac
     5701 tcctcgcacg tgatccggcc gcaaggagac gtgctgttcc agtacctgca ccttcttccc
     5761 gtgctggcgc ccggcgtcat cgtccatatc cacgacgtct tctcgccccg cgactatccg
     5821 gacacctggc tgagggaccg ggtctacttc tggaacgagc agtacctggt cgaggccttc
     5881 ctctcccaca acacggaatg ggagatcctg ctggccggga acatgctgat gcacgaggcc
     5941 tacgacctcc tgaaatcccg gtgccttcac ctcgaccgcg accgggagcc gaggtcgctg
     6001 tatatccgtc gcctgtcgcc ccgggtcccc ggcgcgtgac gcccccgctg tcagcctggt
     6061 cggcgatcgg gcaggtcctg ggaccgggcc gccgggccgg cctggccggg ctcctggcgc
     6121 tgatggtctt cggcatgctg ctggagctgg ccggcgtcgg cctggtcatt cccgtgatcc
     6181 agcttctctc cgggcccgtt ccccgttctg aagcgacgct cgtcggccac tgggcgagcc
     6241 ttgtcggcgc ccgcgatcac gcgggcctgg tgcgcgtcac catgctgtgc ctcgtgatct
     6301 tcttcgcctt caagctggcc tgtcaggcgc tggtcgccct gcgcctcgcc gcctttcgct
     6361 acgacctcca ggcggacatc gcccgtcgcc tctacgccct ctatctggcg cagcctttcg
     6421 gtttccacgt ccggcgaaac tcggcgcacc tgatcagcac ggtgagcaac gaggcgcacg
     6481 acgcggccca ggcgacccac catggcctgg ccctgatcgc ggaactgctg gtgatctgcg
     6541 ggctcgcggt cctgctgatc atcgcctcgc cgctggccgg gatcgccgtc gcttccctcg
     6601 tcgccgttgc ggcctggttc agcctgcgcc tgacccgcac gggcctgaac gcctggggcg
     6661 tacgcctgcg tcatcaccag gccctgaaga tgaagagcct ccagcagggc ctgggtggaa
     6721 taaagctgat caagctgggc ggcctggagg ccgcggccct cgatcacttc gacgggcaca
     6781 acctggcgag ca
//
LOCUS       DAORNX010000121         6758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8361_length_6758_cov_6.958228, whole genome shotgun sequence.
ACCESSION   DAORNX010000121 DAORNX010000000
VERSION     DAORNX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8361_length_6758_cov_6.958228"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..455
                     /locus_tag="PLO65_06850"
     CDS             138..455
                     /locus_tag="PLO65_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRD28002.1"
                     /translation="MAHVTIYTRPFCGYCARALGLLGQKGADFTQISRADHVAAKRVV
                     ELRNHFRRRNIARGHEQPERVVHMANSFLAFRLAYAGEHIGGCDDMLALDREGKLDDL
                     LAA"
     gene            452..1273
                     /locus_tag="PLO65_06855"
     CDS             452..1273
                     /locus_tag="PLO65_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRD28003.1"
                     /translation="MTLRVGLVQTRTPATHAAALAHVVPLVREAAGQGATFILTPEGT
                     NILDRDRDRLLPQLAVLADDPVVGGLRALAAELGVWIDIGSALVRREDGKAANRQVLV
                     NPAGGIVATYDKLHMFDVDLPTGETARESATYEPGDRAVVADVDGVRMGLTICYDMRF
                     AALYRALALAGAKVMTIPAAFTRPTGEAHWEVLLRARAIETGSFVLAAAQGGFHEDGR
                     GTWGRSTIVAPWGEVIGKLDHDDPGVLIADLDLAEADKARAAIPALANARAFTGP"
     gene            1270..1704
                     /locus_tag="PLO65_06860"
     CDS             1270..1704
                     /locus_tag="PLO65_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRD28004.1"
                     /translation="MIRYALACEHGHAFEGWFGTSADFDDQAARGLLECPVCASRAVR
                     KQIMAPAVAGTKAQKSGADLPPAARQMMMEALGKVRAHVEENFDYVGDAFAREARAIH
                     EGQSEERGIYGEATPNEVKSLVEDGVPVAALPPAPPKKSEVN"
     gene            1722..2318
                     /locus_tag="PLO65_06865"
     CDS             1722..2318
                     /locus_tag="PLO65_06865"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRD28005.1"
                     /translation="MSEMMLVFEQVDALTDRLRRLNRPAADMLQPGIAPYDVEDAMMV
                     AGPPPEDLVAMYGRHDGVDLPVGRTLGDGHVIPGHYWMPVAEALEHYKGFAALADLWP
                     ASWFPILTDGGGGYLAVICDPESGDHGCVVEFLPGEDEHEIVFDSVSMMLATAVRCFD
                     EGAYRLKGGFLDEDFEQSGQIADLLNGSRRMVSATDYR"
     gene            complement(2343..2855)
                     /locus_tag="PLO65_06870"
     CDS             complement(2343..2855)
                     /locus_tag="PLO65_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28006.1"
                     /translation="MTIRTARLRLAPARHSDLLAVAAGEAALARALGVTIAPDWAGED
                     AMDAIGRSRDMLADDPDLAQWWMHFIIHEADNRLIGLGGFKGQPAGGQVEIGYEIAPD
                     YRGQGLATEAARGMVDHALAHPTVDHVLAHTLPVESASTTILRRLGLRFIQALTDPDD
                     GPIWRWRLDR"
     gene            complement(2852..3613)
                     /gene="ubiG"
                     /locus_tag="PLO65_06875"
     CDS             complement(2852..3613)
                     /gene="ubiG"
                     /locus_tag="PLO65_06875"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRD28007.1"
                     /translation="MTAPAAELRTSIDPDDVERFSRIAAEWWDPRGKFAPLHRFNPIR
                     LAFIREQALARFGRDGCLRRPFEGLRLLDIGCGGGLLSEPMSRLGFEVTGVDASERNI
                     GTASAHAAEQGLDIAYRASTAEQLLAERAGPFDVILNMEVIEHVADPGEFLRTTARLL
                     APGGLMIVATLNRTIKALALAKIGAEYVLRWVPAGTHDWNKFLTPDELRSFLEGHPFD
                     IEGPFGLAYNPLLGKWVRSPDADVNYLMTVTRPAR"
     gene            3788..5035
                     /locus_tag="PLO65_06880"
     CDS             3788..5035
                     /locus_tag="PLO65_06880"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRD28008.1"
                     /translation="MSRLVMKFGGTSVADLERIRRVGRLVAAEVAAGKQVAVVVSAMA
                     GKTNELVAWTDGAGAAAQGIPASDDEYDMVVASGEQVTAGLLAMTLRNMGLRAKSWLG
                     WQIPIITDEAHGKARIDEIPPEKLAAAMDAGEIAVIAGFQGVTRDGRIATLGRGGSDT
                     SAVAIAAALDAACDIYTDVDGVYTTDPRIENKARKLARISYEEMLEMASLGAKVLQTR
                     SVELAMSQHVPLRVLSSFVEPGEAPGQGTIVCDEEEIVEKRIVSGVAYSRDEAKISLF
                     GLPDTPGVSSKIFGRLADANVNVDMIVQSHARSEGAANMEFTVGKRDAGRTIEILRAA
                     QAEIGFEDAQLNEDVAKVSVIGVGMRSHAGVAKTMFTALAEKGVNIQVISTSEIKISV
                     LIDAAYTELAVRALHAAYGLDQL"
     gene            5182..>6758
                     /locus_tag="PLO65_06885"
     CDS             5182..>6758
                     /locus_tag="PLO65_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRD28009.1"
                     /translation="MVASSFAIRGPRSLLRQIREAMAGDAPAQTKLDMVVRIIAASMV
                     AEVCSIYLRRAGGELELFATEGLSRDAVHVTRLKSGEGLVGEIVRLGRPLNLSDAPHH
                     PAFAYRPETGEDPYHSFLGVPLLRGGRPIGVLVVQNRTERTYGEEEVEDLQIIAMVLA
                     EMVGAGELLSNEALKDVEIAPHKPERLKGARFADGLAYGTVVLHDHPVAPEQLLSDDA
                     TAEEARLGAAIAALQKQIDEMLEGHHGLVGAPYEVLETYRMFAHDRGWNRSLQEAVRS
                     GLTAEAAVERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRHLTGDGHVSRELPDD
                     AILVARNLGPADLLEYDRTRLKGLLLEEGSTASHAAIVARALDIPCVGRLAGLRDKVS
                     QGDAVIVDAETGEAFLRPRPDVVKALNTRLEVRAQRKAQFARLRDTPAFTKDGAKVTL
                     LMNAGLAVDLDILGETGAEGIGLFRTEFQFMVAEDMPRLDAQRSLYERVLDAADGMPV
                     TFRTLDLGGDKLLPYMEL"
BASE COUNT         1061 a         2361 c         2377 g          959 t
ORIGIN      
        1 ggcgccacgg cccacgcctg cgcgcgggcg ctgaaggccg cgggcgccgc cgccgtcgat
       61 ctggccgtca tcgccaaggt gcgcggcggg gcggacacca ctatatgact gtcagcatac
      121 gtcagcgttg agagaccatg gcccacgtca ccatctacac ccgccccttc tgcggctatt
      181 gcgcccgcgc cctgggcctg ctcggccaga agggcgccga cttcacccag atctcccgcg
      241 cggatcatgt agcagccaaa cgcgtagttg agcttcggaa ccatttccgt cggcgaaata
      301 ttgcacgcgg tcatgagcaa cccgagcgcg tcgtgcatat ggctaattcg ttcctcgcct
      361 tccgtcttgc ttacgcgggc gagcacatcg gcggctgcga cgacatgctg gccctcgacc
      421 gtgagggcaa gctggacgac ctgctggccg catgaccctg cgcgtcggcc tggtccagac
      481 ccgcacgccg gccacgcacg cggccgcgct ggcccatgtc gttccgcttg tgcgcgaggc
      541 ggccggccag ggcgcgacct tcatcctgac gccggagggg accaacatcc tcgaccgcga
      601 ccgggaccgg ctgctgcccc agctggcggt actggccgac gacccggttg tcggcggact
      661 gcgggcgctg gccgccgagc tcggcgtgtg gatcgacatc ggctcggcgc tggtccggcg
      721 cgaggatggc aaggccgcca accgccaggt gctggtcaac ccggccggcg ggatcgtggc
      781 gacctacgac aagctgcaca tgttcgacgt cgacctgccg accggcgaaa cggcgcggga
      841 atcggcgacc tacgaacccg gcgatcgcgc cgtcgtggcc gacgtcgacg gcgtccggat
      901 gggcctgacc atctgctacg acatgcgttt cgcggccctg taccgtgcgc tggcgctggc
      961 cggggcgaag gtcatgacca ttcccgccgc cttcacccgc ccgaccggcg aggcccattg
     1021 ggaagtcctg ctgcgcgcgc gggcgatcga gaccggcagt ttcgtgctcg cggcggccca
     1081 gggcggcttc cacgaggacg gccgcggcac ctggggccgt tcgaccatcg tcgcgccctg
     1141 gggcgaggtg atcggcaagc tggatcacga cgaccccggc gtgctgatcg ccgatctgga
     1201 cctggccgag gccgacaagg cccgcgcggc catccccgcc ctggccaacg cccgcgcgtt
     1261 caccggacca tgatcaggta cgccctcgcc tgcgagcatg gccacgcctt cgagggctgg
     1321 ttcgggacct ccgccgactt cgacgaccag gccgcccgcg gcctgctcga atgcccggtc
     1381 tgcgccagca gggcggtgcg caagcagatc atggccccgg ccgtcgccgg gaccaaggcg
     1441 cagaagagcg gcgccgacct gccgccggcc gcgcgccaga tgatgatgga ggcccttggc
     1501 aaggtccgcg cccatgtcga ggagaacttc gactacgtcg gcgacgcctt cgcccgcgag
     1561 gcccgcgcga tccacgaagg ccagtcggaa gaacgcggca tctacggcga ggccaccccg
     1621 aacgaggtga agagcctcgt cgaggacggc gtgccagtcg cggccctgcc gccggcgccg
     1681 ccgaaaaagt cggaggtgaa ctaaatgcgg cgacgtatcg gatgagcgag atgatgctgg
     1741 tgttcgagca ggtcgacgcc ctgaccgacc gcctgcgccg gctgaaccgg ccggccgccg
     1801 acatgctgca gccgggcatc gccccgtatg acgtcgagga cgcgatgatg gtcgccggcc
     1861 cgccgccgga ggacctggtc gcgatgtacg gtcgccacga cggggtcgac ctgccggtcg
     1921 gccgcacgct cggcgacggc catgtgattc ccggacacta ctggatgccc gtcgccgagg
     1981 cgctggaaca ctacaagggg ttcgcggcgc tcgccgacct gtggccggcc agctggttcc
     2041 cgatcctgac cgacggcgga ggcggctatc tggcggtcat ctgcgacccg gagtccggcg
     2101 accatggctg cgtggtcgaa ttcctgccgg gcgaggacga gcacgagatc gtcttcgaca
     2161 gcgtctcgat gatgctggcc accgcggtgc gctgcttcga cgagggcgcc taccggctca
     2221 agggcggctt tctggacgag gatttcgagc aatcgggcca gatcgccgat ctgttgaacg
     2281 ggtcgcggcg gatggtttcg gccacggatt accgctgaac tccggaatgc ggtcggtgag
     2341 gatcaacgat ccagccgcca gcgccagatc gggccgtcgt cgggatcggt cagggcctga
     2401 atgaaccgaa ggcccagccg gcgcaggatc gtcgtcgagg cgctctcgac cggcagggtg
     2461 tgggccagca catggtcgac cgtcggatgg gccagggcgt ggtcgaccat gccccgcgcc
     2521 gcctcggtgg ccagcccctg accgcggtag tccggggcga tctcgtaacc gatctcgacc
     2581 tggccgccgg cgggctggcc cttgaagccg cccagaccga tcagccggtt gtcggcctca
     2641 tgaatgatga aatgcatcca ccactgcgcc aggtccggat cgtcggccag catgtcgcgg
     2701 ctgcggccga tggcgtccat ggcgtcctct cccgcccagt cgggcgcgat ggtgacgccg
     2761 agcgccctgg ccagggccgc ctcgccggcc gccacggcca gcaggtcgct gtgccgcgcc
     2821 ggcgcgagcc tgagacgggc cgtgcggatg gtcatcgggc cgggcgggtc acggtcatca
     2881 ggtagttgac gtccgcgtcg ggcgagcgga cccacttgcc gagcagcgga ttgtaggcga
     2941 ggccgaacgg tccctcgatg tcgaacggat ggccctcgag gaacgagcgc agctcatccg
     3001 gcgtgaggaa cttgttccag tcgtgcgtgc cggcgggcac ccagcgcagc acgtattcgg
     3061 cgccgatctt ggccagggcc agggccttga tcgtgcgatt gagggtggcg acgatcatca
     3121 gtccgcccgg cgccagcagc ctggccgtgg tgcgcaggaa ctcgcccgga tcggcgacgt
     3181 gctcgatcac ctccatgttg aggatcacgt cgaacggccc ggccctttcg gccagcagct
     3241 gctcggccgt cgaggcgcgg taggcgatgt cgagcccctg ttcggccgcg tgggccgagg
     3301 ccgtgccgat gttgcgctcc gacgcgtcga cgccggtcac ctcgaagccc agccggctca
     3361 tcggctcgct gagcaggccg ccgccacagc cgatgtcgag cagcctgagc ccctcgaacg
     3421 gccggcgcag acagccgtcg cggccgaacc gcgccagcgc ctgctcgcgg atgaaggcca
     3481 gccggatcgg attgaacctg tgcagcggcg cgaacttgcc gcgcgggtcc caccactcgg
     3541 cggcgatgcg cgagaaccgt tcgacgtcgt cgggatcgat actggtgcgg agctctgcgg
     3601 cgggcgcggt catgcctcta tctagaacgc cgggggcggg ccgtcgacgg caagcatggc
     3661 ggtcgtgatc tggcgcattg cggcgccgcg aaagcctcgc tagaaggcga cctcttgcgc
     3721 aggcgcggcg ttccaaacgc tgcttcggca agaaaaaacc gccaaacgag ggggcgagcg
     3781 gcctgacgtg tcacggctgg tgatgaaatt cggcggcacc tcggtggccg acctggagcg
     3841 catccgccgc gtcgggcggc tggtcgcggc cgaggtcgcg gccggcaagc aggtcgccgt
     3901 ggtggtctcg gccatggccg gcaagaccaa tgaactggtc gcctggaccg acggcgccgg
     3961 cgcggccgcg cagggcattc cggcgagcga tgacgagtac gacatggtcg tcgccagcgg
     4021 cgaacaggtg accgccggcc tgctggccat gaccctgcgc aacatgggcc tgcgggcgaa
     4081 gtcgtggctc ggctggcaga tcccgatcat caccgacgag gcgcacggca aggcgcgcat
     4141 cgacgagatt ccgcccgaga agctggccgc cgccatggac gcaggcgaga tcgccgtgat
     4201 cgccggcttc cagggcgtca cccgcgacgg ccgcatcgcc accctcggcc ggggcggctc
     4261 ggacaccagc gccgtggcca tcgccgccgc cctggacgcg gcctgcgaca tctacaccga
     4321 cgtggacggg gtctacacca ccgacccgcg catcgagaac aaggcccgca agctggcccg
     4381 catctcctat gaggagatgc tggagatggc gtcgctgggc gccaaggtat tgcagacccg
     4441 atcggtcgag ctggccatgt cgcagcacgt gccgctgcgc gtcctgtcca gcttcgtcga
     4501 gccgggcgaa gcgcccggac agggcaccat cgtctgcgac gaggaggaga tcgtggaaaa
     4561 gcgcatcgtg agcggcgtgg cctacagccg cgacgaggcc aagatcagcc tgttcggcct
     4621 gccggacacg ccgggcgtct cctcgaagat cttcggccgg ctggccgacg ccaatgtcaa
     4681 cgtcgacatg atcgtccaga gccacgcccg cagcgagggc gcggccaaca tggagttcac
     4741 ggtcggcaag cgcgacgcgg gccggacgat cgagatcctg cgcgccgccc aggccgagat
     4801 cggcttcgag gacgcccagc tgaacgagga cgtggccaag gtctcggtca tcggcgtcgg
     4861 catgcgcagt cacgccggcg tggccaagac catgttcacc gccctggccg agaagggcgt
     4921 caacatccag gtcatctcca ccagcgagat caagatcagc gtgctgatcg acgccgccta
     4981 taccgagctg gccgtgcgcg cgctgcacgc cgcctacggc ctggaccagc tctagcctcc
     5041 ggccgggcgc ccgggtccgg cctggccgcg cgtgtcgtgc gctgattgtc gcgacttgac
     5101 gtcgtttgcg cggcgcgggc cactgttccg caacggaaat ctgctatagg cgccgttcag
     5161 gacgggagaa caccaacgcc gatggtcgcg tcgagcttcg ccatacgggg accacgcagt
     5221 ctgctccggc agatccgcga ggccatggcc ggcgacgcgc ccgcccagac caagctggac
     5281 atggtcgtgc ggatcatcgc cgcctcgatg gtggcggagg tctgttcgat ctatctgcgg
     5341 cgcgccggcg gcgagctgga actgttcgcc accgagggcc tgtcgcgcga cgccgtccac
     5401 gtcacccgcc tgaagtccgg cgagggcctg gtcggcgaga tcgtccgact cggccggccg
     5461 ctgaacctca gcgacgcccc gcatcacccg gccttcgcct accgtccgga aacgggcgag
     5521 gatccgtacc actccttcct cggcgtgccc ctgctgcgag gcggccggcc gatcggcgtg
     5581 ctggtggtgc agaaccgcac cgaacgcacc tacggcgagg aggaggtcga ggacctgcag
     5641 atcatcgcca tggtgctggc cgaaatggtc ggcgccggcg aactgctgag caacgaggcg
     5701 ctcaaggacg tcgagatcgc gccgcacaag cccgagcggc tcaagggcgc gcggttcgcg
     5761 gacggcctgg cctacggcac ggttgtgctg cacgatcatc cggtggcgcc cgaacagctg
     5821 ctgtcggacg acgccacggc cgaagaggcg cgtctcggcg ccgcgatcgc cgcgctgcag
     5881 aaacagatcg acgagatgct cgagggccat cacggcctgg tcggcgcgcc ctacgaggtg
     5941 ctcgagacct accgcatgtt cgcccatgac cgcggctgga accggtcgct gcaggaggcg
     6001 gtgcgctcgg gcctgacggc cgaggccgcc gtcgaacgcg tccgcagcga gcaccgcgcc
     6061 aggctgggcc aggcccgcga tccctatctg cgtgagcggc tgcatgacct ggaagatctc
     6121 aacgaccgcc tgctgcggca cctgaccggc gacggccacg tctcgcgcga gctgcccgac
     6181 gacgccatcc tggtggcgcg caacctgggc ccggccgacc tgctcgagta cgaccggacg
     6241 cgactgaagg gcctgctgct cgaggaggga tcgaccgcca gccacgcggc catcgtcgcc
     6301 cgggcgctgg acatcccctg cgtcggccgc ctcgccggcc tgcgcgacaa ggtctcgcag
     6361 ggcgacgcag tgatcgtcga cgccgagacc ggcgaggcct tcctgcggcc gcgcccggac
     6421 gtggtgaagg cgctgaatac ccggctcgag gtccgcgccc agcgcaaggc ccagttcgcc
     6481 cgcctgcgcg acacgccggc cttcaccaag gacggggcca aggtcaccct gctgatgaac
     6541 gcgggcctgg ccgtggacct cgacattctc ggcgagacgg gcgcggaagg catcggcctg
     6601 ttccgcaccg agttccagtt catggtcgcc gaggacatgc cgcgcctgga cgcccagcgg
     6661 tcgctctacg aaagggtgct cgacgcggcc gacggcatgc ccgtgacctt ccgcacgctc
     6721 gacctggggg gcgacaagct gctgccctac atggagct
//
LOCUS       DAORNX010000122         6723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8424_length_6723_cov_7.135423, whole genome shotgun sequence.
ACCESSION   DAORNX010000122 DAORNX010000000
VERSION     DAORNX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8424_length_6723_cov_7.135423"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLO65_06890"
     CDS             complement(<1..284)
                     /locus_tag="PLO65_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRD28010.1"
                     /translation="MIGRLRGVVAEIGEEEALIDVGGVGYVVRCGSRTLGRLPAIGDE
                     TVVHVEHQWSEAQGPRLYGFSTREERSVFVLLQAIQGVGPKAAMAVLDVLP"
     gene            complement(281..784)
                     /gene="ruvC"
                     /locus_tag="PLO65_06895"
     CDS             complement(281..784)
                     /gene="ruvC"
                     /locus_tag="PLO65_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRD28011.1"
                     /translation="MMNAIRILGLDPGLRRTGWGVIAVEGSRLTWVAHGVITSDDKAS
                     LAERLLSLFDGIDAVIAAHAPHEAAVEETFVNANPQSTLKLGQARAAAMIAPAKAGLP
                     VAEYAAREVKKCVVGTGAADKGQIAFMVARLLPTAGGPTQDAADALAVAITHAHSRRA
                     RALRQVA"
     gene            complement(991..1701)
                     /locus_tag="PLO65_06900"
     CDS             complement(991..1701)
                     /locus_tag="PLO65_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRD28012.1"
                     /translation="MDQFLNIALNPQYILPLVGALLAGGCIGFERGFRGQPAGFRTHS
                     LLALTSALLMVAANHQMMWIGASTPHDVIRIDPVRMAHGILTGVGFLGGGVIFREGFN
                     VHGLTTAASIWTTSCIGILFGIGFWEIAVSGTVLALLVLAGFRLIDQRLPQQSLADIT
                     IRYGRGAAPGAEELRERLRALGLKSAPLSERLLKKGQFIEHGATVSALNRSAIDRLAA
                     ELRADHKVIEFEIEPRNA"
     gene            1902..3326
                     /locus_tag="PLO65_06905"
     CDS             1902..3326
                     /locus_tag="PLO65_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD28013.1"
                     /translation="MAIGRTAIAGAVMAAALPALAGAQSTPAPRPDQLAFRAIYKELV
                     EINTTLSVGSCTAAAEAMATRLKAAGYADSDLHMIVDPAHPKEGNLVAVLPGTDPKAK
                     AVLLLAHIDVVEANRADWTRDPFTLVEEDGWFYARGATDDKAQASIWVDTMIRLRQEA
                     ARPRRTVKMALTCGEESEGALNGAELLATKYRDLIDAGFALNEGAGGLLGDDGKPVSL
                     SIQSGEKVYQDFTLSSTNPGGHSSRPLHDNAITHLAGALVRIGAYDFPVQLNETTRAY
                     FTAMAPLVEPETGAAMRALVADPTDAAAAAVIARNPTWNSMLRTTCVATQAAAGHAPN
                     ALPQRAEANVNCRIFPGVTVEAVQAQLTTLAADPKVTITPDATTRLTPPPPPLSPAIM
                     KPLRAEADKLWPGVPIVPFQSTGATDGKYLNSYGIPTYGLTGIFAGNENSGAHGLNER
                     VRVKSLYDARDFLYGLVKTYAAAK"
     gene            3338..4831
                     /locus_tag="PLO65_06910"
     CDS             3338..4831
                     /locus_tag="PLO65_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD28014.1"
                     /translation="MTFLRGLLATAVALSLTGAAGAQTATPPAVVRPDQLAFKALYKE
                     LVEINTTLSVGSCTRAAEAMGARLTAAGFPAADVQVVVNPDHPREGSLVAVLHGTDAK
                     AGPMLLLAHIDVVEANPADWTRDPFTLVEENGYFFARGASDDKAQAAIWVDSLIRLKQ
                     AGFRPRRDIKMALTCGEESEAYNGIEDLVTNHRPLVDAAFALNEGADGLLDENDKPVV
                     LEVQGGEKVYQDFTLTITNPGGHSSRPVKDNALTRLSAALVRIGAYDFPIAFNDATRG
                     YFTRMIPLVPADKGAAMKALVANPDDPAAQAVVTADPMWNSMLRTTCVATMVSAGHAP
                     NALPQRATANVNCRILPGVPVEAVRQQLMAIADDPDIAFTLAHEPSKASPPPPLTPFI
                     MGPIEKEAAKLWPGVPVVTILLTGATDGVHTNAAGIPTYGVSGIFGSSDGDGIHGLNE
                     RVRVKALYDGRDFLFALIKDYAMAKDGPAGGKQPGMLGRLMGRVKGG"
     gene            complement(4939..5697)
                     /locus_tag="PLO65_06915"
     CDS             complement(4939..5697)
                     /locus_tag="PLO65_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRD28015.1"
                     /translation="MAGHSKFKNIMHRKGRADAVRSKLFSKLSREITVAAKAGLPDPN
                     MNPRLRLAVANAKAESVPKDVIERAIKKSQMGDAADYTEIRYEGVAPGGVGVIVEVLT
                     DNKNRAAANVRTIFGKLGGNMGATGSVTFNFDRMGQIEYPIKAASEDAMMEAAIEAGA
                     ADVEADLGEDGSGHTVYTAFEDLNEVAQALEGQFGEASSTKIVWRPRSLVPVSGDQAA
                     TVMKLLEALEDDDDVQNVYSNADISDEDAASFAG"
     gene            complement(5765..6601)
                     /locus_tag="PLO65_06920"
     CDS             complement(5765..6601)
                     /locus_tag="PLO65_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRD28016.1"
                     /translation="MRFAFFGDVVGRSGRDGLADHLPGLRRRLGLEFVVINAENAASG
                     FGITENTARELFDAGADCLTLGNHSWDQKEALTYIVREPRLIRPANYPILSDTPGKGS
                     HLFQTQGGRTVFVVNLLGRVHMDAMDDPFAAADRELDKAPLGQVADAVILDMHCEATS
                     EKMAMGHYCDGRASLVVGTHTHVPTADSQILPGGTAYQTDAGACCDYDSVIGNQKEEP
                     LRRFTTKISQGRYQPASGPATVCGVFVETDDRTGLARRVEPIRIGGRLREHVPVVELA
                     TA"
     gene            complement(6602..>6723)
                     /locus_tag="PLO65_06925"
     CDS             complement(6602..>6723)
                     /locus_tag="PLO65_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRD28017.1"
                     /translation="AVGFAYSGQETPGLPREPHDQLLDGILTEAGYIAARKDF"
BASE COUNT         1026 a         2362 c         2329 g         1006 t
ORIGIN      
        1 ggcagcacgt cgagcacggc catggccgcc ttgggcccga cgccctggat ggcctgcagc
       61 agcacgaaca cgctgcgctc ctcgcgcgtg gaaaagccgt acaggcgcgg gccctgggcc
      121 tcgctccact ggtgctcgac atggaccacg gtctcgtcgc cgatcgccgg cagccggccc
      181 agggtgcgcg acccgcaacg cacgacatag ccgaccccgc cgacatcgat cagcgcctct
      241 tcctcgccga tctcggcgac gacgccgcgc agccgcccga tcatgccacc tgcctcaacg
      301 cgcgggcccg gcgggagtgg gcgtgggtga tggccacggc cagggcgtcg gcggcgtcct
      361 gggtcggacc gccggccgtc ggcaacagcc gggcgaccat gaaggcgatc tggcccttgt
      421 cggcggcgcc cgtgccgacg acgcatttct tgacctcgcg ggcggcgtat tcggccacgg
      481 gcagtccggc cttcgccggg gcgatcatcg ccgccgcgcg ggcctggccc agcttcaggg
      541 tcgactgcgg attggcgttg acgaaggtct cctcgacggc ggcctcgtgc ggcgcatggg
      601 cggcgatcac cgcgtcgatg ccgtcgaaca ggctcagcag ccgctcggcc agcgaggcct
      661 tgtcgtcgga ggtgatgacg ccatgggcga cccacgtcag gcgcgagccc tcgaccgcga
      721 tcacgcccca gccggtgcgc cgcaggcctg gatcgagccc gagaatccga atcgcgttca
      781 tcatgtgttc tactactgtg gcccagtggt gaacgaagcg caaaggctct catgttcgat
      841 gctccccgct tgcggggcgc tgtcgcgcgg agcgtgacta cgccgctgtg ccgccgctgc
      901 cggaatgacc ggaattcagg ttggggccgc tcccatccgc gccgccccct ccaccgcctc
      961 cccgcgatgc ggggaggatc agcctcaagt ctaggcgttc cgtggctcga tctcgaattc
     1021 gatgaccttg tggtcggcgc gcaactcggc ggccagccgg tcgatggcgc tgcggttgag
     1081 ggcgctgacg gtggcaccgt gctcgatgaa ctggcccttc ttcaacaggc gttcgctgag
     1141 cggagcggac ttgagcccca gcgcgcggag ccgctcgcgc agctcctcgg ctcccggcgc
     1201 cgcgccccga ccgtagcgga tggtgatgtc ggccagcgac tgctgcggca gccgctggtc
     1261 gatcagccgg aacccggcca gcaccagcag cgccagcacc gtgccgctca ccgcgatctc
     1321 ccagaagccg atgccgaaca ggatgccgat gcagctggtg gtccagatcg aggccgccgt
     1381 ggtcaggcca tggacgttga agccctcgcg aaagatcacc ccgccgccga ggaagccgac
     1441 gccggtcaaa atgccgtggg ccatgcgcac ggggtcgatg cggatcacgt cgtgcggcgt
     1501 cgaggcgccg atccacatca tctggtgatt ggccgccacc atcagcaggg ccgaggtcag
     1561 ggccagcagg ctgtgggtgc ggaagccggc aggctggccg cgaaagccgc gctcgaaacc
     1621 gatgcaaccg ccagccagaa gcgctccgac caggggcagg atatattggg gattcaacgc
     1681 gatgttcaga aactggtcca tcaaactcct ccgaacccgg aacaatgcgc tgattggcgg
     1741 aggatgcaaa tcgccgggga catgtacctc tcgtacgtgt cccctactca cctcgccggg
     1801 ataggggaca cgtaccgaag tcggtacatg tccccggcga cccttgcgcc gcccccgcgg
     1861 atgggtgtga aactggccgc tcagtcgagg gggggcgagt catggctatc gggcggacgg
     1921 cgatcgcggg tgcggtcatg gcggcggcct tgccggccct ggcgggggcc cagtcgaccc
     1981 ccgctcctcg ccccgaccag ctggcctttc gcgcgatcta caaggagctg gtggagatca
     2041 atacgaccct gtcggtgggc agctgcaccg ccgccgccga ggccatggcc acgcgcctga
     2101 aggcggccgg ctatgccgac agcgacctgc acatgatcgt cgacccggcc catccgaagg
     2161 aaggcaacct ggtcgccgtg ctgcccggga ccgacccgaa ggccaaggcc gtcctgctgc
     2221 tggcgcatat cgacgtggtc gaggccaacc gggccgactg gacccgtgac ccgttcaccc
     2281 tggtcgagga ggacggctgg ttctacgccc gcggcgccac cgacgacaaa gcccaggcct
     2341 cgatctgggt cgacaccatg atccgcctca ggcaggaggc cgcgcggccc cgccggaccg
     2401 tgaagatggc cctgacctgc ggcgaggaga gcgagggcgc cctgaacggc gccgagctgc
     2461 tggcgacgaa ataccgcgac ctgattgacg ccggtttcgc gctcaacgag ggcgccggcg
     2521 ggctgctggg cgatgacggc aagccggtgt cgctgtcgat ccagtcgggc gagaaggtct
     2581 accaggactt caccctgagc tcgaccaacc ccggcggtca ctccagtcgg ccgctgcatg
     2641 acaacgccat cacccatctg gccggagccc tggtgcggat cggcgcctat gacttcccgg
     2701 tgcagctgaa cgagacgacg cgggcctatt tcacggccat ggctccgctg gtcgagcccg
     2761 agacgggggc ggccatgcgc gccctggtcg ccgacccgac cgacgcggcg gccgccgccg
     2821 tgatcgcccg taatccgacc tggaactcga tgctgcggac cacctgcgtc gccacccagg
     2881 ccgccgccgg ccacgccccc aacgccctgc cccagcgggc cgaggccaac gtcaactgcc
     2941 gcatcttccc gggcgtgacg gtcgaggcgg tgcaggccca gctgaccacc ctggccgccg
     3001 acccgaaggt cacgatcacg cccgacgcga caacccggct gacgccgccg ccgccgcccc
     3061 tgtcgccggc catcatgaag cccctgcggg ccgaggccga caagctgtgg ccgggcgtgc
     3121 cgatcgtgcc gttccagtcg accggggcca cggacggcaa gtacctcaac agctacggca
     3181 tcccgaccta cggcctgacc ggcatcttcg cgggcaatga gaacagcggc gcgcacgggc
     3241 tgaacgagcg ggtccgggtc aagtcgctct atgacgcccg ggacttcctc tacggcctgg
     3301 tcaagaccta cgccgcggcg aaatagggac aatggcgatg actttcctgc gcggactcct
     3361 ggcgaccgcc gtcgccctgt cgctgaccgg cgccgccggg gcccagacgg cgacgccgcc
     3421 ggccgtggtc cggcccgacc agctggcctt caaggcgctc tacaaggagc tggtcgagat
     3481 caacaccacc ctgtcggtgg gcagctgcac cagggccgcc gaggccatgg gcgcccggtt
     3541 gacggccgcc ggcttcccgg ccgccgacgt gcaggtggtg gtcaaccccg accatccgcg
     3601 cgagggcagc ctggtggccg tgctgcacgg gaccgacgcg aaggccgggc cgatgctgct
     3661 gctggcccac atcgacgttg tcgaggccaa tccggccgac tggacccgcg atccgttcac
     3721 cctggtcgag gagaacggct atttcttcgc ccgcggcgcc tcggacgaca aggcccaggc
     3781 ggccatctgg gtcgacagcc tgatccggct gaagcaggcc gggttcaggc cgcgccggga
     3841 catcaagatg gccctgacct gcggcgagga gagcgaggcc tataatggca tcgaggatct
     3901 ggtgaccaac caccggccgc tggtcgacgc cgccttcgcg ctcaacgagg gggccgacgg
     3961 cctgctggac gagaacgaca agccggtggt gctcgaggtc cagggcggcg agaaggtcta
     4021 ccaggacttc accctgacga tcaccaatcc cggcggccat tcgagccggc cggtcaagga
     4081 caacgccctg acccggctgt cggcggccct ggtcaggatc ggcgcctacg atttcccgat
     4141 cgccttcaac gacgccacgc gcggctactt cacccgcatg atcccgctgg tcccggccga
     4201 caagggcgcg gcgatgaagg cgctggtggc caatccggac gacccggcgg cccaggctgt
     4261 ggtgacggcc gacccgatgt ggaacagcat gttgcggacg acctgcgtgg cgaccatggt
     4321 cagcgccgga cacgcgccca acgccctgcc gcagcgggcc acggccaacg tcaactgccg
     4381 catcctgccc ggcgtgccgg tcgaggcggt gcgccagcag ctgatggcca tcgccgacga
     4441 tccggacatc gccttcaccc tcgcccatga gccgagcaag gcctcgcctc cgccgccgct
     4501 gacgcccttc atcatggggc cgatcgagaa ggaggcggcg aagctgtggc cgggcgtgcc
     4561 ggtggtgacc atcctgctga ccggggccac cgacggggtg cacaccaacg ccgccggcat
     4621 tcccacctac ggcgtcagcg gcatcttcgg cagttcggac ggcgacggca tccacgggct
     4681 gaacgagcgc gtccgggtca aggcgctcta cgacggccgc gacttcctgt tcgccctgat
     4741 caaggactac gccatggcga aggacgggcc ggcgggcggg aagcagcccg gaatgctggg
     4801 gcggttgatg ggccgggtga agggcggctg acgcaggttg cgtggttcga cacgcgcttc
     4861 tcgctgctca ccatgacgtg gcgctcacgc acgtcatcct gagcaggccg cgcagcggcc
     4921 gtgtcgaagg acgcaccatc accccgcgaa ggacgccgcg tcctcgtcgc tgatgtccgc
     4981 gttggaatag acgttctgca cgtcgtcgtc gtcctccagc gcctcgagca gcttcatcac
     5041 cgtcgcggcc tggtcgccgc tgaccgggac gagcgacctg ggccgccaga cgatcttggt
     5101 cgaggacgcc tcgccgaact gaccttccag cgcctgggcg acctcattga ggtcctcgaa
     5161 ggcggtgtag acggtgtggc ccgagccgtc ctcgcccagg tcggcctcga cgtcggcggc
     5221 gccggcctcg atggccgcct ccatcatcgc gtcctcgctg gcggccttga tcgggtactc
     5281 gatctggccc atgcggtcga agttgaaggt caccgagccg gtggccccca tgttgccgcc
     5341 cagcttgccg aagatggtcc gcacgttggc cgcggcgcgg ttcttgttgt cggtcagcac
     5401 ctcgacgatg accccgacgc cgccgggcgc gacgccttcg tagcggatct cggtgtagtc
     5461 ggccgcgtcg cccatctgcg acttcttgat cgcccgctcg atgacgtcct tgggcaccga
     5521 ctcggccttg gcgttggcca cggccaggcg cagccgcggg ttcatgttcg ggtcgggcag
     5581 accggccttg gccgccacgg tgatttcccg cgacagcttg gagaacagct tggaccgcac
     5641 cgcatcggcg cgccccttgc ggtgcatgat gttcttgaac tttgagtggc cggccatggg
     5701 gcggtgtcgg tcccggtgtt gaaagggtgc gggcttttag cgtcggtgcg gggcggcgcc
     5761 taggtcacgc cgtcgccagc tccaccaccg gcacatgttc cctcagccgg ccgccgatgc
     5821 ggatcggttc gacgcggcgg gcgaggcccg tgcggtcgtc ggtctcgacg aacacgccgc
     5881 agaccgtcgc ggggccggac gcgggctggt agcggccctg gctgatcttg gtggtgaacc
     5941 ggcgcagcgg ctcttccttc tggttgccga tcacgctgtc gtagtcgcag caggccccgg
     6001 cgtcggtctg gtaggccgtg ccgccgggca ggatctggct gtcggccgtc ggcacatggg
     6061 tgtgggtgcc gaccaccagg ctggcgcggc cgtcgcagta gtggcccatg gccatcttct
     6121 cgctggtcgc ctcgcagtgc atgtcgagga tcaccgcgtc ggccacctgg cccagcggag
     6181 ccttgtccag ctcgcggtcg gcggcggcga acgggtcgtc catggcgtcc atgtgcaccc
     6241 ggcccagcag gttgacgacg aacaccgtcc ggccgccctg ggtctggaac aggtggctgc
     6301 ccttgcccgg ggtgtcggac aggatcgggt agttggccgg ccggatcagc ctgggctcgc
     6361 ggacgatgta ggtcagcgcc tccttctggt cccagctgtg gttgcccagc gtcaggcagt
     6421 cggcgccggc gtcgaacagc tcgcgggcgg tgttctcggt gatgccgaag ccggacgcgg
     6481 cgttctcggc gttgatcacc acgaactcga ggcccaggcg gcggcggagg cccggcagat
     6541 ggtcggccag gccgtcgcgg ccggagcggc cgacgacgtc gccgaagaag gcaaagcgca
     6601 ttcagaagtc ctttcgggct gcaatatacc cggcctcggt cagaatccca tcgagcagct
     6661 ggtcgtgcgg ctcgcggggc aggccggggg tctcctggcc gctgtaggca aagcccacgg
     6721 ccg
//
LOCUS       DAORNX010000123         6646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8555_length_6646_cov_7.206342, whole genome shotgun sequence.
ACCESSION   DAORNX010000123 DAORNX010000000
VERSION     DAORNX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8555_length_6646_cov_7.206342"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLO65_06930"
     CDS             <1..378
                     /locus_tag="PLO65_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRD28018.1"
                     /translation="VAGRLLRRVRDFAAADGAPLIDKGHAARALARLEVDEAGLDSLD
                     RRYMRALIENYGGGPAGVETLAYAIAEARDAVEDVIEPFLLQQGFIQRTPRGRVACGK
                     AYLHLGLQEPPAPKGTPGLFDEG"
     gene            1479..1949
                     /gene="ybgC"
                     /locus_tag="PLO65_06935"
     CDS             1479..1949
                     /gene="ybgC"
                     /locus_tag="PLO65_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRD28019.1"
                     /translation="MTTASPTTDFPTAGRFEGREHILPVRIYYEDTDFSGLVYHANYV
                     RYFERGRSDFLRLAGVSHTDLLERHDPAAFVVTRMEIDFKRPAKIDDALQVITTYDQA
                     RGARLQVSQRIVRDGELIAAARVSAACISLDGRPRKPPAGLVDALRPWFCKDEG"
     gene            2030..2728
                     /gene="tolQ"
                     /locus_tag="PLO65_06940"
     CDS             2030..2728
                     /gene="tolQ"
                     /locus_tag="PLO65_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRD28020.1"
                     /translation="MDAQTISADTFSIVTLWSNADWVVRLVMVGLALASLWSWAVIID
                     KLFRFGALNAEANRFEDKVGSGRPLEDVGQESAGSKHPLPKLLNAALREWKEARSRGA
                     IGDTQTALLIQRIDRVLDSNIARESQKVEDGLGSLAIVATASPFIGLFGTVWGIMHAF
                     QNIAIEKNTSLAVVAPAIAEALFATAIGLAAAIPAYIAYNKFSTDAGKFAARLEGFAD
                     DLSTAIQRRLAEKI"
     gene            2732..3241
                     /locus_tag="PLO65_06945"
     CDS             2732..3241
                     /locus_tag="PLO65_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HRD28021.1"
                     /translation="MALSSTDAFGAVGKRRSRRGRGRRGALSEINVTPLVDVMLVLLI
                     IFMISAPLLTSGVPIDLPKTEAGAMQDQSEPITLTIKADGALFVGENEIPFAALKPRL
                     QSMASAGYDKPIYVRADGKASYEIVARVMAALSTGGFSKINLITDTGGPSSGAALAPA
                     STAEGQPAP"
     assembly_gap    3401..3561
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3562..4119
                     /locus_tag="PLO65_06950"
     CDS             <3562..4119
                     /locus_tag="PLO65_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28022.1"
                     /translation="EPMPEPEPVPVAPPPPDAKPTPPVKTPPKPSPQPARKPSLDLDR
                     LMADVQGARRPPRAQTGGGGGTGKKDRTLAVGASRGPSAGAKGYLLTLGTDLGRRWNP
                     NCMVEGGADVNIKVSFIVSSNGRLLTDPVSSGKDAPPGSLIRAGSDAAVRAIRMTEPF
                     PNFPPELYGEKITVNFNANQVCANR"
     gene            4133..5434
                     /gene="tolB"
                     /locus_tag="PLO65_06955"
     CDS             4133..5434
                     /gene="tolB"
                     /locus_tag="PLO65_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRD28023.1"
                     /translation="MTPRIVLAALCAIFLAGAALPGVARADIEIDVNQGEVRPLPIAI
                     PAFSGAGRGAEMAQVIAGNLDRSGLFAPIDAAAFPERNLDIGIQPDFAGWKAINAQAL
                     VNGAITVEADGKLRVDFRLWDVFSGEQLLGLQFTSTPENWRRVAHKISDAVYERLTGE
                     KGYFDTRVVFVAESGPRGARVKRLMIMDQDGANPSSLTDGSYVVMTPRFSSTSQEITY
                     MALRPSGSSIYLFNIETARQETLGRFPGMVFAPRFSPDGGRVAFSVERGGNSDIWLMN
                     LATRGQVKLTSDPGIDTSPSFSPDGTRIAFNSDRGGSPQIYVMGADGSGARRISYGTG
                     RYMTPVWSPNGDFIAFTKQTGGTFHIGVMRPDGSDERILTTSYLDEGPTWAPNGRVLM
                     FFRETPGGSPRLWTVDITGRILRPAPYQGSGSDPAWSPLLN"
     gene            5563..6108
                     /gene="pal"
                     /locus_tag="PLO65_06960"
     CDS             5563..6108
                     /gene="pal"
                     /locus_tag="PLO65_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRD28024.1"
                     /translation="MSFDVKRAARLALIVAATASMAACASKPKPGPGPGPSAGPGPAG
                     PRGPGPQAPVDTRPLPGSVQDFVINVGDFVYFDLDRYDIRSDAMPVLDAQAAWLRQYP
                     NVRVRIEGNCDERGTREYNLALGARRANAVRDYLASRGVASSRIATLSWGKERPIDPG
                     SNEDAWARNRNGHTVIVEGAR"
     gene            6222..>6646
                     /locus_tag="PLO65_06965"
     CDS             6222..>6646
                     /locus_tag="PLO65_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRD28025.1"
                     /translation="MKRNTILATLAVLTVSLGAAAAWSQTPMPDPLDDRSVRRLDKME
                     KVVRELRSIVFQGRDTGKPVVVMPAETESQIEALTARVADLEETLTRLNGTNETLTFE
                     LEQARKALRASDATTRSLADRLAALEGRTAQIEAAAAPTP"
BASE COUNT         1034 a         2334 c         2131 g          986 t
ORIGIN      
        1 gtggccggcc ggctgctgcg ccgggtgcgc gacttcgccg ccgccgacgg ggccccgctg
       61 atcgacaagg gccacgcggc ccgggccctg gcgcggctgg aggtcgacga ggccggactc
      121 gacagcctcg accgccgcta catgcgcgcc ctgatcgaga actacggcgg cggcccggcg
      181 ggggtcgaga ccctggccta cgccatcgcc gaggcgcgcg acgcggtcga ggacgtcatc
      241 gagcccttcc tgctgcagca gggcttcatc cagcgcaccc cgcgcggccg ggtggcgtgc
      301 gggaaggcct atctgcacct gggcctgcag gaaccgccgg ctccgaaggg gacgccgggc
      361 ctgttcgacg aggggtagtg cgtccttcga cacggccctt cgggcctgct caggatgacg
      421 tgcagctgaa ccacgtcatg gtgagcagcg gcgaagccgc gtgtcgaacc acgcaacctg
      481 gcgccgcgcc gcgacaccct gcgtccttag acacggtccc gttgggccct gctcaggatg
      541 acgtgcagct gttccacgtc atggtgagca gcggcgaagc cgcgtgtcga accacgcaac
      601 ctggcgccgc gccgcgacac cctgcgtcct tagacacggt cccgttgggc cctgctcagg
      661 atgacgtgca gctgttccac gtcatggtga gcagcgccgc agccgcgtgt cgaaccaggc
      721 aatctggcgc cgcgcggtga caccctgcgt ccttcgacac ggcccttcgg gcctgctcag
      781 gatgacgtgc agctgttcca cgtcatggtg agcagcgccg caggcgcgtg tcgaaccacg
      841 caacgtgatt gcggtggggc gcgagacaac ctaaagatgt cctatgcggc tgcaaccatc
      901 ctggggctac atcctgctct gcgcagacgg cagctattac atcggctgca cgacaaatct
      961 tgaacaacgg ctgggccagc acagcgcagg ctcggttgcg ggctacacgt cgacgcgttt
     1021 gcctctccga tgcgtctaag tcgtcgagtt ccagtcgctt tacgacgcca tcgcctggga
     1081 acgtcgggtc aagcgctggt cgcgggcaaa gaagacggcg ttgatcgcac gggcctacga
     1141 ccggttgccc gatctggcgc gcagctacca gcatcatggt cgtcctcgct gaaccgaatt
     1201 gcgtccttcg acacgggccc cttgggccct gctcaggatg acgtgggtct gtgccgatct
     1261 ccctccacgt catggtgatc agcgcgaagc gcgtgtcgaa ccacgcaacc tggcgccgcg
     1321 ccgcgacacc ctgcgtcctt cgacacggct cttcgggcct gctcaggatg acgtggaggg
     1381 gtgggcgctg gcgggtgagc gggatgacga caccaccgca atccactacg aacgtcatcc
     1441 tgagcagcgc ctgaaaggcg cgcgtcgaag gatgcagtgt gaccaccgcc agcccgacca
     1501 ccgacttccc caccgccggc cgcttcgagg gtcgcgagca tattctgccc gtccgcatct
     1561 actacgagga caccgacttc agcgggctgg tctaccacgc caactacgtg cgctatttcg
     1621 agcgggggcg gagcgacttt ctgcggctgg cgggggtgtc gcacacggac ctgctggagc
     1681 ggcatgatcc ggcggccttt gtcgtcacgc ggatggagat cgacttcaag cggccggcga
     1741 agatcgacga cgccctgcag gtcataacga cctacgacca ggccaggggc gcgcggctgc
     1801 aggtcagcca gcgcattgtc cgcgacgggg agctgatcgc cgcggcccgg gtgtcggcgg
     1861 cctgcatcag tctcgatgga aggccgcgca agccgccggc gggacttgtc gatgcgctgc
     1921 ggccctggtt ttgcaaggat gagggttaag gcccgttaag ggctgcgcca tagtttcacc
     1981 aaagccgcac gcgtcaattg cggccagcct gcccggggga tgtgaccgca tggacgccca
     2041 gacgatttcg gccgacacct tttccattgt caccctgtgg agcaacgccg actgggtggt
     2101 gcgcctggtc atggtcggtc tggccctggc ctcgctgtgg tcctgggcgg tgatcatcga
     2161 caagctgttc cgtttcgggg cgctcaacgc cgaggccaac aggttcgagg acaaggtcgg
     2221 ttcgggccgg ccgctcgagg atgtcggcca ggagtccgcg ggatcgaagc atccgctgcc
     2281 caagctgctc aacgccgccc tgcgcgaatg gaaggaagcc cgttcgcgcg gcgccatcgg
     2341 cgacacccag acggccctgc tgatccagcg catcgatcgg gtgctcgaca gcaacatcgc
     2401 ccgtgagagc cagaaggtcg aggacggcct cggcagcctg gccatcgtcg ccacggcctc
     2461 gccgttcatc ggcctgttcg gcacggtctg gggcatcatg catgcgttcc agaacatcgc
     2521 catcgagaag aacacctcgc tggcggtcgt ggccccggcc atcgccgagg ccctgttcgc
     2581 cacggccatc ggcctggcgg cggccatccc ggcctacatc gcctacaaca agttctcgac
     2641 cgacgccggc aagttcgccg cccggctgga aggcttcgcc gacgacctgt cgacggccat
     2701 ccagcgccgc ctggcggaga agatctgagc catggccctg tcgtccacag acgccttcgg
     2761 cgcggtcggc aagcggcgct cgcggcgtgg ccgcggccgg cgtggcgcgc tcagcgagat
     2821 caacgtcacc ccgctggtcg acgtgatgct ggtgctgctg atcatcttca tgatcagcgc
     2881 cccgctgctg accagcggcg tgcccatcga tctgcccaag accgaggccg gcgcgatgca
     2941 ggaccagtcc gagccgatca ccctgaccat caaggccgac ggcgccctgt tcgtcggcga
     3001 gaacgagatt ccgttcgcgg cgctgaagcc gcggctgcag tcgatggcca gcgccggata
     3061 cgacaagccg atctatgtcc gcgccgacgg caaggcctcc tacgagatcg tcgcccgggt
     3121 gatggccgcc ctgtccaccg gcggcttcag caagatcaac ctgatcaccg acaccggcgg
     3181 acccagctcc ggcgcggcgc tcgcgcccgc gtccacggcc gagggccaac ccgcgccgtg
     3241 atggaccgtc tcctccgatc gccccggcga aggcgggggt cccggccctg ccgccgcctg
     3301 ggttccagcc tcggcggaag ccgtcggtcc gggagtgggt cgtgaaccgg cgcgagacca
     3361 ccagcatgtc gccggccctg ctggcctcgc tgctcatgca nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn ngagccgatg ccggagcccg aacccgttcc cgtcgccccg
     3601 ccgccgcccg acgccaagcc gacgccgccc gtcaagacgc cgcccaagcc gtcgccccag
     3661 ccggcccgca agccgtcgct cgatctcgat cggctgatgg ccgacgtgca gggcgcccga
     3721 cggccgcccc gggcccagac cggcggcggc ggcggaaccg gcaagaagga tcgcaccctg
     3781 gcggtcggcg ccagccgtgg cccgtccgcc ggcgccaagg gctatctgtt gacgctgggc
     3841 acggacctgg gccgccgctg gaatcccaac tgcatggtcg aggggggcgc cgacgtgaac
     3901 atcaaggtgt cgttcatcgt ctcgtccaac ggccggctgc tgaccgaccc ggtatcctcg
     3961 ggcaaggacg ccccgcccgg ttccctcatc cgggccggat ccgatgcggc cgttcgcgcc
     4021 atacgaatga cagaaccctt ccccaatttc ccgcccgagc tctacggtga gaagatcacc
     4081 gtcaatttca acgccaatca ggtctgcgcc aatcgctgac cggagcgcca cgatgacccc
     4141 cagaatcgtc ctcgccgccc tctgcgccat cttcctggcc ggcgccgccc tgcccggggt
     4201 cgcccgggcg gacatcgaga tcgacgtcaa ccagggcgag gtgcgcccct tgccgatcgc
     4261 cattcccgcc ttctcgggcg ccggccgcgg cgcggagatg gcccaggtca tcgccggcaa
     4321 tctcgaccgg tccggcctgt tcgcgccgat cgacgccgcc gccttccccg agcgcaacct
     4381 cgacatcggc atccagcccg acttcgccgg ctggaaggcg atcaacgccc aggccctggt
     4441 caacggcgcg atcaccgtcg aggccgacgg caagctgcgg gtcgacttcc gcctgtggga
     4501 cgtgttttcc ggcgagcagc tgctgggcct gcagttcacc tcgacgcccg agaactggcg
     4561 ccgcgtcgcg cacaagatca gcgacgcggt ctatgagcgg ctgaccggcg agaagggcta
     4621 tttcgacacc cgcgtggtgt tcgtcgccga gagcggcccg cgcggcgccc gcgtcaagcg
     4681 gctgatgatc atggaccagg acggggccaa tccctcgagc ctgaccgacg gcagctatgt
     4741 cgtgatgacg ccccgcttct cctcgaccag ccaggagatc acctacatgg cgctgcggcc
     4801 cagcggctcc agcatctatc tgttcaacat cgagacggcg cggcaggaaa ccctgggacg
     4861 gttccccggc atggtgttcg ccccgcggtt ctcccccgac ggcgggcgcg tggccttctc
     4921 ggtcgagcgc ggcggcaaca gcgacatctg gctgatgaac ctggccaccc ggggccaggt
     4981 caagctgacc tccgatcccg gcatcgacac ctcgcccagc ttctcgcccg acggaacccg
     5041 catcgccttc aattccgacc gcggcggcag cccgcagatc tacgtcatgg gcgcggacgg
     5101 ctcgggcgcg cggcgcattt cctacggaac cggtcgctac atgaccccgg tgtggagccc
     5161 gaacggcgac ttcatcgcct tcaccaagca gacgggcggc accttccaca tcggcgtgat
     5221 gcgtccggac ggctcggacg agcggatcct gaccaccagc tacctcgacg agggcccgac
     5281 ctgggccccc aacggccgcg tcctgatgtt cttccgtgaa acgcccggcg gatcgccgcg
     5341 tttatggacc gtcgacatca ccggccggat cctgcggccc gcgccctacc agggttcggg
     5401 ctcggatccg gcctggtctc cattgctcaa ctgatcggtg attgtgattg ttcggcctca
     5461 gactgacgcc attgtgaggc acagtcagag ttttcagtcg cgttgatcgg cattctggcg
     5521 tcactattgg cgcgatggat cgtttccgga ggaggaagcc gcatgagctt cgacgtcaag
     5581 cgcgccgcta gactggcgct tattgtcgcc gccaccgcct cgatggccgc ctgcgcctcg
     5641 aagcccaagc ctgggcccgg acctggacct tcggcgggcc ccggaccggc cggaccgcgc
     5701 ggccccgggc cgcaggcgcc ggtcgacacg cgccccctgc ccggctcggt gcaggacttc
     5761 gtgatcaacg tcggcgactt cgtctatttc gacctcgacc gctacgacat ccgctccgac
     5821 gccatgccgg tgctcgacgc ccaggccgcc tggctgcggc agtatccgaa cgtgcgggtg
     5881 cgcatcgagg gcaactgcga cgaacgcggc acccgcgaat acaacctggc cctcggcgct
     5941 cgccgcgcca acgccgtgcg tgactacctg gcgagccgcg gcgtcgcctc cagccgcatc
     6001 gccaccctgt cctggggcaa ggagcggccg atcgacccgg gttcgaacga ggatgcctgg
     6061 gcgcgcaacc gcaacggcca caccgtcatc gtcgagggcg cgcgatagcg cccgcctgac
     6121 aggtgaatgc cgggaggggg ccgcgcgggg ggcgcggtcc cctctttgca ttcaggacca
     6181 acgccgtaat ttcgacgccg caacagacgc atcatcgggc catgaagcga aacaccatcc
     6241 tcgccaccct cgccgtcctg accgtctccc tgggggccgc cgccgcctgg tcgcagacac
     6301 cgatgcccga ccctctggac gaccggtcgg tccgccggct ggacaagatg gagaaggtcg
     6361 tgcgcgagct gcggtcgatc gtcttccagg gccgcgacac cggaaagccg gtggtggtca
     6421 tgcccgccga gaccgaaagc cagatcgagg cgctgaccgc ccgggtcgcc gacctggaag
     6481 agaccctgac ccggctgaac gggacaaacg agaccctgac cttcgagctg gaacaggccc
     6541 gcaaggccct gcgcgcgtcg gacgccacga cacggtcgct ggccgaccgg ctggccgcgc
     6601 tcgaggggcg caccgcccag atcgaggccg cggccgcgcc gacgcc
//
LOCUS       DAORNX010000124         6454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8892_length_6454_cov_6.734646, whole genome shotgun sequence.
ACCESSION   DAORNX010000124 DAORNX010000000
VERSION     DAORNX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8892_length_6454_cov_6.734646"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLO65_06970"
     CDS             <1..882
                     /locus_tag="PLO65_06970"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD28026.1"
                     /translation="AQQIADGSASVVVAGGQESMSQSPHAANIRNGQKMGDLGFVDTM
                     IKDGLWDAFHGYHMGQTAENIAVRWQITREDQDRFAVTSQNRAEAAQKAGKFAGEIAP
                     VTIKGRKGDTVVDQDEYIRHGATYESMAGLRPAFTKDGTVTAANASGLNDGAAALVLM
                     SADEAKKRGLTPLARIASWASAGVEPDIMGTGPIPAMKKALDKAGWSVADLDLIESNE
                     AFAAQSLSVVRELGLDPAKTNVNGGAIAIGHPIGASGARILTTLLHEMKRSGAKKGAA
                     TLCVGGGMGVAMCVEAV"
     gene            1070..1795
                     /gene="phbB"
                     /locus_tag="PLO65_06975"
     CDS             1070..1795
                     /gene="phbB"
                     /locus_tag="PLO65_06975"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRD28027.1"
                     /translation="MSRVAFVTGGTRGIGKAIVERLKADGMKVAAGYSGNDKAAAETA
                     EELGVLVVKGNVGNFEDCARAVKIAEDALGPIDVLVNNAGITRDGFFHKMTYEQWGEV
                     IRVNMDSAFNMTRPVIEGMRERNWGRIVNISSINGQKGQVGQTNYSAAKAGLIGFTKA
                     LALENAKKGITVNVICPGYIDTDMVSAVPENVLAGIIAGIPVGRLGKGEEIADMVSYL
                     AGERAGFVTGATFTLNGGQYLAS"
     gene            1992..2765
                     /locus_tag="PLO65_06980"
     CDS             1992..2765
                     /locus_tag="PLO65_06980"
                     /inference="COORDINATES: protein motif:HMM:NF027577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HRD28028.1"
                     /translation="MASSKSQFPAVAAVAAATLMLALLCVAAPAFAQEGSLKDFLFGD
                     RRGESQRQAPPPPVARYVSETGEGFILDRSSERTLLRFENSPEIWVLQPQIGPRGDVI
                     YKNDIGQVLLRATRVGGLTLFTGKRPTGASASLAGASTPIRLKVIGPVDLYRILLASS
                     ARVSRASRHRISFDAEATPASSSLIADAAVLSVMAIERVAQRPEGQAMAARIQRVVIV
                     EGARPAVALRAGTLLIAVTPSLGVAGRPSSDRIANVTMK"
     gene            complement(2752..3183)
                     /locus_tag="PLO65_06985"
     CDS             complement(2752..3183)
                     /locus_tag="PLO65_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD28029.1"
                     /translation="MTAAEIVALLDLAPHPEGGWFRETFRDTAGPSGRGASTAIYFLL
                     EAGQESRWHRVDAAEVWHFHAGAPLRLSVGPAAMVLGADLSAGERPQIVVPAGVWQQA
                     RSLGAWTLVGCTVAPGFDFAGFEMAPDGWTPPGSATGPISS"
     gene            complement(3180..3932)
                     /gene="gloB"
                     /locus_tag="PLO65_06990"
     CDS             complement(3180..3932)
                     /gene="gloB"
                     /locus_tag="PLO65_06990"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRD28030.1"
                     /translation="MPLTVHQFPCLSDNYGFLVRDEATGQAACIDTPDAAAILRELKT
                     LGWTLTHILNTHWHPDHAGGNAEIKAATGCQVVGPAEVERLGTAPDRVVDEGDVVMLG
                     ETRFDVINTGGHTLGHITFHDAADRIAFVGDTLFALGCGRLFEGTPQQMWASLAKLTA
                     LPDSTAVYCAHEYTASNARFALSVDHDPALKARAEAIFAARERGDWTVPTTIGLEKAT
                     NPFLRAPLLRPEIADPAEAFGAVRAAKDSFRG"
     gene            4085..4873
                     /locus_tag="PLO65_06995"
     CDS             4085..4873
                     /locus_tag="PLO65_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD28031.1"
                     /translation="MRRDVLELRAFYASARGRAAREMVGRKVAEAWGDPRGLDLLGVG
                     YATPYLERFHERARRTVAVMPATQGVEVWPAGGRNLACLADETALPLANALFDRVLCV
                     HALEESDSATALMQEVCRVLAPSGRVIVAVAARNGLWSNAEGTPFGHGRPWSRRQLEQ
                     LLRDADLEPSGWTRALYAPPFAWTAGWAEGFEQIGAWLWPPFAGLILMEAVKQTFAVK
                     PRAARARARVFVPGTLVPVPPAPAGRASDTAMQQDPLAAEPVSP"
     gene            4915..5259
                     /locus_tag="PLO65_07000"
     CDS             4915..5259
                     /locus_tag="PLO65_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD28032.1"
                     /translation="MKMIIAVIKPSRLDAVLDGVTEAGASGLTVTEVRGYGRQKGKTE
                     VYRGAEYEVKLLPKVKLEIAAPDDVVPAVLDAIVRTANTGKIGDGKIFVLDLEQVLRI
                     RTGETGAAAIAG"
     gene            5420..>6454
                     /locus_tag="PLO65_07005"
     CDS             5420..>6454
                     /locus_tag="PLO65_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRD28033.1"
                     /translation="MADILRDGDTCWRTARTDALAFLVDGADYFAAVKAAMSAARRSI
                     WLLAWVFDPLTRLEPDRAGRSGDPRTADRLGLLLRRMAALNPALDVRILAWDMPFPIN
                     ASQLFAPQRAMAEFMGSKVRFRLDSTLPASACHHQKVLVIDGRLAFVSGGDLGADRWD
                     TCDHVDHDPRRRLPNLRSYPARHDVSVMLEGPAARDCAQLFADRWASAGAGHLDVPEA
                     DFSVESPWPPSVTPDLIGHAVSLVRTHPGWKHEPEVTEGLRLHLAAIASARRCIYLEN
                     QYLTSPTIVAALSRRLAEADGPEVVTVGPARSPSFFDRLAMDSARMAAIDALGAADVH
                     GRFRALTARTA"
BASE COUNT          983 a         2288 c         2261 g          922 t
ORIGIN      
        1 gcccagcaga tcgccgacgg ctcggccagc gtcgtcgtcg ccggcggcca ggagagcatg
       61 agccagtcgc cgcacgcggc caacatccgc aacggccaga agatgggcga cctgggcttc
      121 gtcgacacca tgatcaagga cggcctgtgg gacgccttcc acggctacca catgggccag
      181 acggccgaga atatcgccgt gcgctggcag atcacccgcg aggaccagga ccgtttcgcc
      241 gtcacctcgc agaaccgcgc cgaggcggcc cagaaggccg gcaagttcgc cggcgagatc
      301 gccccggtga cgatcaaggg ccgcaagggc gacaccgtgg tcgaccagga cgaatacatc
      361 cgccacggcg cgacctacga aagcatggcg ggcctgcgcc cggccttcac caaggacggc
      421 acggtcaccg cggccaacgc ctcgggcctc aatgacggcg ccgcggccct ggtgctgatg
      481 agcgccgacg aggcgaagaa gcgcggcctg accccgctgg cccgcatcgc cagctgggcg
      541 agcgccggcg tcgagcccga catcatgggc accgggccga tcccggcgat gaagaaggcc
      601 ctcgacaagg ccggctggtc cgtcgcggac ctggacctga tcgagtccaa cgaggccttc
      661 gccgcccagt cgctgtcggt ggtccgcgag ctgggcctcg acccggccaa gaccaacgtc
      721 aacggcgggg ccatcgccat cggccacccg atcggcgcct cgggcgcgcg catcctgacc
      781 acgctgctgc acgagatgaa gcggtccggc gcgaagaagg gcgccgcgac cctctgcgtc
      841 ggcggcggca tgggcgtggc catgtgcgtc gaggctgtct gaggactcga cccttctccc
      901 cttgcgggag aaggtgtcag gcggagcctg gcggatgagg ggtctcaccc cattcaatgg
      961 tcggaccggc gggtttgaag tggccttcaa cccctcatcc gaccgctaac gcggccacct
     1021 tctcccgcaa ggggagaagg aagacaaaaa aagagcaggg aagacgcgca tgagcagggt
     1081 tgcattcgtc acgggcggga cccgcggcat cggcaaggcg atcgtcgagc ggctgaaagc
     1141 cgacggcatg aaggtcgcgg ccggctattc gggcaatgac aaggccgccg ccgagaccgc
     1201 cgaggaactg ggcgtgctgg tggtcaaggg caatgtcggc aacttcgagg actgcgcccg
     1261 cgcggtgaag atcgccgagg acgcgctggg acccatcgac gtgctggtca acaacgccgg
     1321 catcacccgt gacggtttct tccacaagat gacctacgag cagtggggtg aggtcatccg
     1381 cgtgaacatg gacagcgcct tcaacatgac ccgcccggtc atcgagggca tgcgtgagcg
     1441 caactggggc cgcatcgtca acatcagctc gatcaacggc cagaagggcc aggtcgggca
     1501 gaccaactac tcggcggcca aggccggcct gatcggcttc accaaggccc tggccctcga
     1561 gaacgccaag aagggcatca cggtcaatgt gatctgcccg ggctacatcg acaccgacat
     1621 ggtctctgcc gtgccggaga acgtgctggc cggcatcatc gccggcatcc ccgtcggccg
     1681 ccttggcaag ggcgaggaga tcgccgacat ggtctcctac ctggccggcg aacgcgcggg
     1741 cttcgtgacc ggggcgacgt tcaccctgaa cggcgggcaa tatctggcga gctagggccc
     1801 gttcctctcc cgcgagggag agggcgacca cgcggagcgt ggggcagagg gaagggctgc
     1861 cgctcgacgc tggatcgcca ccgcgacgtc acgcaccaag gcgcgtggtc ctcgcaatcg
     1921 tccgtcgtcc gcttgcccac cgcctccggc ccgtccaaga atcgccccag tccccgtgca
     1981 tggctgaggg aatggcgagt tcgaaaagtc agtttcctgc agtcgcggcc gtcgccgcgg
     2041 cgacgctgat gctggcgctg ctgtgcgtgg cggcgccggc cttcgcgcag gaaggctccc
     2101 tgaaggattt cctgttcggc gacaggcgtg gtgagagcca gcgtcaggcg cccccgcccc
     2161 ccgtcgcccg ctacgtctcc gaaaccggcg agggcttcat cctcgaccgt tcgagcgagc
     2221 gtacgctgct gcgcttcgag aacagccccg agatctgggt gctgcagccg cagatcggcc
     2281 cgcgcggcga cgtgatctac aagaacgaca tcggccaggt gctgctgcgg gcgacccgcg
     2341 tcggcggcct gaccctgttc accggcaagc gcccgaccgg ggcctcggcc tcgctggcgg
     2401 gcgcctccac cccgatccgc ctcaaggtca tcgggccggt cgacctctac cgcatcctgc
     2461 tggcgtcgag cgcccgggtc agtcgcgcct cgcgccaccg gatcagtttc gacgccgagg
     2521 cgacgccggc ctcgtcatcg ctgatcgccg acgccgccgt gctgtcggtg atggcgatcg
     2581 agcgggtcgc gcaacgtccg gaaggccagg ccatggcggc ccggatccag cgggtggtga
     2641 tcgtcgaggg cgcccgtccg gcggtcgccc tgcgcgccgg caccctgctg atcgccgtca
     2701 cgcccagcct cggcgtggcc ggccggccgt cgtcggaccg catcgccaat gtcacgatga
     2761 aataggcccg gtcgccgatc ccgggggcgt ccagccatcc ggcgccatct cgaatccggc
     2821 gaagtcgaat cccggcgcca cggtgcagcc gaccagggtc caggccccga gagagcgagc
     2881 ctgttgccag acgccggccg gaaccacgat ctgtggccgt tcgccggccg aaaggtctgc
     2941 ccccagcacc atcgcggctg gtccgacgct cagacgcagc ggcgcgccgg cgtgaaaatg
     3001 ccagacttcg gccgcgtcca cccggtgcca gcggctctcc tgcccggcct cgagcaggaa
     3061 gtagatcgcc gtcgaggcgc cccgccccga cggaccggcc gtgtcgcgaa aggtctcgcg
     3121 gaaccagccc ccctcgggat ggggcgcgag gtccagcagg gcgacgatct ccgccgccgt
     3181 catccccgga agctgtcctt ggccgctcgc accgcgccga aggcctcggc cggatcggcg
     3241 atctcgggcc gcagcagcgg cgcgcgcagg aacgggttgg tggccttctc caagccgatg
     3301 gtcgtgggca ccgtccagtc gcccctctcg cgggccgcga agatcgcctc ggcccgggcc
     3361 ttgagcgcgg gatcgtgatc caccgacagc gcgaaccgcg cgttcgaagc cgtgtattca
     3421 tgggcgcagt agacggccgt gctgtccggc agggcggtca gtttcgccag gctggcccac
     3481 atctgctgcg gcgtgccctc gaacagccgg ccgcagccga gggcaaacag cgtgtcgccg
     3541 acgaaggcga tccggtcggc ggcgtcatga aaggtgatgt gccccagggt gtgcccgccg
     3601 gtgttgatga cgtcgaaccg ggtctcgccg agcatcacca catcgccctc gtcgaccacc
     3661 cggtccggcg cggtcccgag tcgctcgacc tcggccgggc cgaccacctg gcagccggtg
     3721 gccgccttga tctcggcgtt gccgcccgcg tggtccggat gccagtgggt gttgaggatg
     3781 tgcgtcaggg tccagcccag cgtcttcagc tcgcgcagga tggcggcggc gtctggcgtg
     3841 tcgatgcagg ccgcctggcc ggtggcctcg tcgcggacga gaaagccgta gttgtcggac
     3901 aggcagggaa actggtggac ggtgaggggc atgcggagtc ctcattgcgt ggttcgacac
     3961 gcgccttcgg cgctgctcac catgacgtgg tttagcggat tgcattagac tacgtcatcc
     4021 tgagcagccg cgcagcggcg tgtcgaagga cgtaaccccg tccaatgcca tcgaactgag
     4081 tcccatgcgc cgcgatgttc tggagctccg cgccttctac gcctcggccc gcgggcgggc
     4141 ggcgcgggaa atggtcggcc gcaaggtggc cgaggcctgg ggcgatccgc gcgggctgga
     4201 cctgctcggg gtcggctacg cgacccccta tctggaacgt tttcacgagc gcgcgcgccg
     4261 caccgtggcg gtgatgccgg cgacccaggg cgtcgaggtc tggcccgctg gcggtcgcaa
     4321 cctggcctgc ctcgccgacg agacggccct gcccctggcc aacgccctgt tcgaccgcgt
     4381 gctgtgtgtc cacgcgctcg aggagagcga ctccgccacg gccctgatgc aggaagtctg
     4441 ccgcgtgctg gccccgtccg gccgggtgat cgtcgcggtc gccgcccgca atggcctgtg
     4501 gtccaacgcc gagggcaccc cgttcggcca cggccggccg tggagccggc ggcagctgga
     4561 gcagctgctg cgcgacgccg acctggagcc ctcgggctgg acccgcgccc tctacgcccc
     4621 gccgttcgcc tggaccgccg gctgggccga gggattcgaa cagatcgggg cctggctttg
     4681 gccgccgttc gccggactga tcctgatgga ggcggtcaag cagaccttcg cggtgaagcc
     4741 ccgggcggcg cgcgcccggg cccgcgtgtt cgtcccggga accctggtgc ccgtcccgcc
     4801 ggcgccggcc gggcgggcgt ccgataccgc aatgcagcaa gaccccttgg cggccgagcc
     4861 tgtttcgccc tagcttgggc ttcagccccc gcccgcgcca tcggagtcgt cgccatgaaa
     4921 atgatcatcg ccgtcatcaa gccgagccgc ctggacgcgg tgctcgacgg ggtgaccgag
     4981 gcgggcgcct cgggcctgac cgtcaccgag gtgcgcggct acggccgcca gaagggcaag
     5041 accgaggtct atcgcggcgc cgagtacgag gtgaagctgc tgcccaaggt caagctggag
     5101 atcgccgccc ccgacgacgt tgtcccggcg gtgctcgacg ccatcgtgcg cacggccaac
     5161 accggcaaga tcggcgacgg caagatcttc gtcctcgacc tggaacaggt gctgcgcatc
     5221 cgcacgggcg agacgggcgc ggcggcgatc gcggggtagg gaatagggag tagcgagtag
     5281 ggagtagagg aacacagggg cgcggtgcga ccggcgggca agctctgcta ttccctagtc
     5341 cctattccct actccctccc cgcctcggcg ccctttcccg ccctcgcgcg ttgaagcgga
     5401 ggatcactga cggggcggca tggcggacat tctgcgggac ggcgacacct gctggcggac
     5461 agccaggacg gacgccttgg ccttcctggt cgacggcgcg gactatttcg cggccgtgaa
     5521 ggccgccatg agcgcggccc ggcgctcgat ctggctgctg gcctgggtgt tcgacccgct
     5581 gacccggctc gagccggacc gcgccggccg cagcggcgac ccgcgcaccg ccgaccgcct
     5641 gggccttctg ctgcggcgga tggcggcgct caatccggcc ctcgacgtgc gcatcctggc
     5701 ctgggacatg ccgttcccga tcaacgcctc gcagctgttc gccccgcagc gggcgatggc
     5761 cgagttcatg ggatcgaagg tcaggtttcg gctcgactcg accttgccgg ccagcgcctg
     5821 ccaccaccag aaggtgctgg tcatcgacgg gcgactggcc ttcgtcagcg gcggcgacct
     5881 cggcgccgac cgctgggaca cctgcgacca cgttgaccac gacccgcgcc gccgactgcc
     5941 caacctgcgc agctatccgg cccggcacga tgtctcggtg atgctcgagg gccctgccgc
     6001 gcgcgactgc gcccagctgt tcgccgaccg ctgggccagc gcgggcgcgg gccatctcga
     6061 cgtgccggag gcggatttct ccgtcgaatc gccgtggccg ccgtcggtca cgccggacct
     6121 gatcggtcat gccgtcagcc tggtgcgcac ccatccgggc tggaagcacg agccggaagt
     6181 gaccgagggc ctgcgcctgc atctggcggc gatcgcctcg gcgcgccgct gcatctatct
     6241 ggagaaccag tacctgacct cgccgacgat cgtcgcggcc ctgtcccggc ggctggccga
     6301 ggcggacgga ccggaggtgg tgacggtcgg cccggcccgc agcccgagct tcttcgaccg
     6361 gctggcgatg gacagcgccc gcatggccgc catcgacgcc ctcggcgcgg cggacgtcca
     6421 cgggcggttc cgcgccctga ccgcccggac ggcg
//
LOCUS       DAORNX010000125         6448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8904_length_6448_cov_5.692789, whole genome shotgun sequence.
ACCESSION   DAORNX010000125 DAORNX010000000
VERSION     DAORNX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_6448_cov_5.692789"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLO65_07010"
     CDS             <1..232
                     /locus_tag="PLO65_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28034.1"
                     /translation="TEQAALRGSVGAAPAPAAEEPPLTFPTPVLPASTPLPASDGGQC
                     RLTCSRDYYFCLAGEDDRCPQQWSRCVTGCDG"
     gene            318..1685
                     /locus_tag="PLO65_07015"
     CDS             318..1685
                     /locus_tag="PLO65_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD28035.1"
                     /translation="MADRLPKACIIGAGCSGFTTAKRLKDVGVPYDCFEVSDEIGGNW
                     YYKNPNGMSACYESLHIDTSKWRLAFEDFPVPADWPDFPHHAQLFQYFKDYVAHFGLR
                     ETITFNTAVTKARRNAEGLWEVTLSTGETRLYDVLFVCNGHHWSPRIPDYPGEFDGPA
                     FHSHAYRDPFDPVDMRGKNVVVVGMGNSAMDIASELAQRPIAKNLWVSARRGVWVLPK
                     YMNGKPADKTAMPHWMPRKLGLSLARKTIRKTLGNMEDYGLPRPDHEPLEAHPSVSGE
                     FLTRAGCGDIKFKPAIKALEGKQVRFADDTVEDVDAIVFATGYDMRFPFFDDPDLVPD
                     ADHQLPLFKRMMKPGIPNLFYMALAQPLPTLVNFAEQQTKLVAAYLTGRYLPPSEAEM
                     KAVTVRDEAIHKGQYYASKRHTIQVDFGVYVHDLMKEIARGEKRARAAGNPLPVKARA
                     EAVGA"
     gene            complement(1682..2344)
                     /locus_tag="PLO65_07020"
     CDS             complement(1682..2344)
                     /locus_tag="PLO65_07020"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28036.1"
                     /translation="MTGRTASTSSAPRRQSAAARRERARARPGEILNAARDIFSRKGF
                     EAARMDEVAAAAGVTKPAVYRYFPGKDRLIEALLEEDLAIPFRRLSDWVEGHDGSIED
                     LVAGFADRVGSLQGSGLTRGYLMLALDEGGRRPEIADFIRNQILAPGLVVLAGAFHRA
                     MARGELAAGHDPGFMARMFFAPFLQTSLGTVGYALPVGDEAERARYRAFHTAAFLRAF
                     RA"
     gene            2418..3071
                     /locus_tag="PLO65_07025"
     CDS             2418..3071
                     /locus_tag="PLO65_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD28037.1"
                     /translation="MTHSIDVFWSFRSPYSWLATPALVKMQADWDLDIRVRPVLPIAV
                     RIDGFFKRVNPLWPPYLARDVYRLGQMQGTPIRWPRPDPIVMDISTGEVQADQPYIHR
                     ITRMGQAAAEAGKGLAYIAEAGRVIWGGEIEGWHEGDHLARAAERAGLDPDALERTAR
                     DEADRLDAAIAANQQALEGAGHWGVPTMVFEGEPFFGQDRLDVLLWRLKQNGLQPRT"
     gene            3148..3771
                     /locus_tag="PLO65_07030"
     CDS             3148..3771
                     /locus_tag="PLO65_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28038.1"
                     /translation="MSHVAVLTSGKREQTKVANRRAILDAAREVFAELGYESATVRDI
                     IRRTGLASGTFYNYFRSREEVFEALADDGAARFRPILRACWEASNSFDGYLRAAIQAY
                     FDFLADEHESWQAHRPAGERHPRMTVQTPEMERVFEEVRDAFLMAIERGGAPRVDADY
                     LAAACIAVARQVGDQMLERRPIDTVAATDFATRLILGGLPALPRLAT"
     gene            3784..4830
                     /locus_tag="PLO65_07035"
     CDS             3784..4830
                     /locus_tag="PLO65_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28039.1"
                     /translation="MADPAVQKAILKLMLALPTPVLRMMSGGGVVWQGGRTLDPRFQF
                     LAAGARRMPPMSSLTPEEARAASAGGLAMMAGKPEPGVRTEALSIEGPGGPLRLRAYR
                     PADQDSAAPLMVFAHFGGGVIGDLETCDAFCGMLARIARTAVLSVDYRLAPEHRFPAG
                     LDDVLAAYRWGRDNATRFGAAPGVASIGGDSMGGNFAAIIAQDLKRAGEPQPALQLLI
                     YPAVDVASETQSMTTYADAYPLSRDTMNWFMGHYMGPDADPADPRLSPIKTEDLSGLA
                     PAIVVTAGFDPLTDQGECYAKRLKDAGVPVIYRCYDSLAHGFTAFTGAIPAADTACRE
                     IAGFVRQAAEGMLK"
     gene            4926..5909
                     /locus_tag="PLO65_07040"
     CDS             4926..5909
                     /locus_tag="PLO65_07040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRD28040.1"
                     /translation="MRALVVDSLGPDYAGVSLKDIPVPSPAAGEVLVAVKAASVNFPD
                     LLMTRGEYQLKPPLPFVGGMELAGEIVALGEGVEAFAVGDAVVGGAKLGGFAAFATLP
                     AATIRRKPDNLTFSQAAAIGAAYLTAWVALVRRARIEPGEWVLVHGAAGGVGLAAVDL
                     ARALGARVIAASASDEKLKIVADAYAPDAVVNVTGGFRDKVRAITGGAEADVIYDPVG
                     GEVFDESVRCIAFDGRLLVVGFTSGRIPTISVNMPLIKGFSVVGVRAGEYGRQFPDRG
                     RENMDAVWKLAAEGVIRPRVHAELPLERWREAFDLLADRKVVGKAILRPDL"
     gene            6019..6156
                     /gene="rpmH"
                     /locus_tag="PLO65_07045"
     CDS             6019..6156
                     /gene="rpmH"
                     /locus_tag="PLO65_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRD28041.1"
                     /translation="MSKRTFQPSRLVRKRRHGYRTRMATKSGQKIVARRRAKGRKRLT
                     A"
     gene            6206..>6448
                     /locus_tag="PLO65_07050"
     CDS             6206..>6448
                     /locus_tag="PLO65_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRD28042.1"
                     /translation="MDEKPNLERLKKRPDFLAAARAPSCARGAVVVQRRDRADGDPVI
                     RAGFTATKRVGGSVVRNRARRRLREAARLLLPQTGRP"
BASE COUNT          991 a         2302 c         2186 g          969 t
ORIGIN      
        1 gacggagcag gccgccctgc gcggatcggt cggcgcggcc ccggcgccgg cggccgagga
       61 gcctccgctg acgtttccga cgccggtcct gccggcctcg acgcccctcc ccgcgagcga
      121 tggcggccag tgccggctga cctgttcccg cgactactat ttctgcctcg ccggcgagga
      181 tgaccgttgt ccccagcagt ggagccggtg cgtcactggc tgcgacggat aggcgccagg
      241 gcgccgctgc gtcacggttg acgcgtacgt cactctggct aggctcgcct gaacatcgat
      301 aagtcaggga gatccgcgtg gccgaccgcc tgcccaaggc ctgtatcatt ggcgccggct
      361 gctccggctt caccaccgcc aagcgactca aggatgtcgg cgtcccgtac gactgcttcg
      421 aggtctccga cgagatcggc ggcaactggt actacaagaa ccccaatggc atgtcggcct
      481 gctacgagag cctgcatatc gacacctcga agtggcggct ggctttcgag gatttccccg
      541 tgccggcgga ctggcccgat ttcccgcacc acgcccagct gttccagtac ttcaaggatt
      601 atgtcgccca cttcggcctg cgcgagacga tcaccttcaa cacggcggtg acgaaggcgc
      661 ggcgcaacgc ggaaggcctg tgggaggtga ccctctcgac cggcgagacg cggctctacg
      721 acgtcctgtt cgtctgcaac ggacaccact ggagcccgcg cattcccgac tatccgggcg
      781 agttcgacgg tccggccttc cacagccacg cctatcgcga cccgttcgac ccggtcgaca
      841 tgcggggcaa gaacgtggtg gtcgtcggca tgggcaattc ggcgatggat atcgccagcg
      901 aactggccca gaggccgatc gcgaagaacc tgtgggtctc ggcccggcgc ggcgtctggg
      961 tgctgcccaa gtacatgaac ggcaaaccgg ccgacaagac cgccatgccc cactggatgc
     1021 cgcgcaagct gggcctgagc ctggcccgca agaccatccg caagaccctc ggcaacatgg
     1081 aagactacgg cctgccgagg cccgatcacg agccgctcga ggcccacccc tcggtcagcg
     1141 gcgagttcct cacccgcgcc ggatgcggcg acatcaagtt caagccggcg atcaaggcgc
     1201 tggagggcaa gcaggtccgg ttcgccgacg atacggtcga ggacgtcgac gcgatcgtct
     1261 tcgccaccgg ctacgacatg cgcttcccgt tcttcgacga tcccgatctg gtcccggacg
     1321 cagaccacca gctgccgctg ttcaagcgga tgatgaagcc gggcatcccc aacctgttct
     1381 acatggcgct ggcccagccg ctgccgacgc tggtcaattt cgccgaacag cagaccaagc
     1441 tggtcgcggc ctatctgacc ggccgatacc tgccaccgtc cgaggccgag atgaaggcgg
     1501 tcacggtccg cgacgaagcg atccacaagg gccagtacta tgcgtccaag cggcacacga
     1561 tccaggtcga cttcggcgtc tatgtccacg acctgatgaa ggagatcgcc cgcggcgaga
     1621 agcgcgcgag ggcggccggc aatccgctgc ccgtcaaggc gcgggcggag gcagtcggcg
     1681 cctaggcccg gaacgcccgc aggaacgccg ccgtgtggaa cgcgcggtag cgggcgcgct
     1741 cggcctcgtc gccgaccggc agggcgtagc ccaccgtgcc cagcgaggtc tgcaggaacg
     1801 gcgcgaagaa catccgcgcc atgaaacccg gatcatggcc ggcggccagc tcgccacgcg
     1861 ccatcgcccg atggaaggcg ccggccagca ccacgagacc cggcgcgagg atctggttgc
     1921 ggatgaagtc ggcgatctcc ggccggcgac caccctcgtc cagggccagc atcaggtagc
     1981 cgcgggtcag gccgctgccc tgcaggcttc cgaccctgtc ggcgaatccg gccaccagat
     2041 cctcgatgga cccgtcgtga ccctccaccc agtccgacag ccggcggaac ggaatcgcca
     2101 ggtcctcctc cagcagcgcc tcgatcagcc ggtccttgcc ggggaaatag cgatagaccg
     2161 ccggcttggt gaccccggcg gcggcggcca cctcgtccat ccgggcggct tcgaacccct
     2221 tgcgcgaaaa gatgtcccgg gcggcgttga ggatctcgcc cgggcgcgcc cgcgcccgtt
     2281 cgcgccgggc cgcggcggac tgccggcgcg gggctgagga cgtcgatgcg gttcggccgg
     2341 tcatggaatc tcccgtggat cgcatcctag cgttctgtat actcgtgggt aactaaagac
     2401 ttcccggaga agccgccatg acccacagca tcgatgtctt ctggtcgttc cgcagtccct
     2461 attcctggct ggcgacgccg gcgctggtga agatgcaggc ggactgggac ctggacatcc
     2521 gggtgcggcc ggtgctgccc atcgccgtgc gaatcgacgg cttcttcaag cgggtgaacc
     2581 cgctctggcc gccctatctg gcccgcgacg tctaccggct gggccagatg cagggcacgc
     2641 cgatccgctg gccccggccc gacccgatcg tcatggatat ttccacggga gaagtgcagg
     2701 ccgatcagcc ctacatccac cgcatcaccc gcatgggcca ggcggcggcc gaggccggca
     2761 agggactggc ctatatcgcc gaggccggcc gggtcatctg gggcggcgag atcgagggct
     2821 ggcacgaggg ggaccacctg gcccgggccg ccgagcgggc gggtctcgac cccgacgcgc
     2881 tcgagcgcac cgccagggac gaggctgacc ggctggatgc ggccatcgcc gccaaccagc
     2941 aggccctgga aggcgccggc cactggggcg tgccgaccat ggtcttcgag ggcgagccct
     3001 tcttcggaca ggaccggctg gatgtgcttc tgtggcggct gaaacagaat ggcctccagc
     3061 cccggacctg aatccaccgc acaggatatg acagggcgtc aacttccggc ttcccgccgg
     3121 gcgcggcgac ctgatagctt tgcttcgatg agtcacgtcg ccgttctgac atcgggaaaa
     3181 cgcgagcaga ccaaggtcgc caaccgacgg gcgatcctgg acgccgcgcg cgaggtgttc
     3241 gccgagctcg gctatgagtc ggccaccgtg cgcgacatca tccggcgcac cggcctggcg
     3301 tccggaacct tctacaacta cttccgctcc cgcgaggagg tcttcgaggc gctcgcggac
     3361 gacggcgcgg cgcggttccg gccgatcctg cgcgcctgct gggaagcgtc caacagcttc
     3421 gacggttacc tgcgcgcggc gatccaggcc tatttcgact ttctcgccga cgagcacgag
     3481 tcctggcagg cccatcgccc ggccggtgag cgccacccgc ggatgaccgt gcagaccccg
     3541 gagatggagc gggtgttcga ggaggtgcgc gacgccttcc tgatggccat cgagcggggc
     3601 ggagccccgc gggtggacgc cgactacctg gccgccgcct gcatcgccgt ggcgcgccag
     3661 gtcggcgacc agatgctcga gcggcggccg atcgacacgg tcgcggccac cgatttcgcg
     3721 accagactga tcctgggcgg attgcccgcc ctgccacgcc tggcgacctg accgagagac
     3781 cgcatggccg accccgccgt ccagaaggcc atcctgaagc tcatgctggc gttgccgacg
     3841 ccggtgcttc gcatgatgtc gggcggcggt gtcgtctggc agggcggtcg caccctcgat
     3901 ccgcggttcc agtttctcgc ggccggcgcc cggcgcatgc cgccgatgtc gtccctgacg
     3961 ccggaggaag cccgcgcggc cagcgccggc ggcctggcga tgatggccgg caaacccgag
     4021 cccggcgtgc gcaccgaagc cctgtcgatc gaggggccgg gcggccccct ccgcctgcgc
     4081 gcctatcgtc ccgccgacca ggattcggcg gccccgctga tggtgttcgc ccacttcggc
     4141 ggcggggtga tcggcgatct ggagacctgt gacgccttct gcggcatgct cgccagaatc
     4201 gcgcgaacgg cggtgctgtc cgtcgattac cggctggcgc ccgagcaccg tttcccggcc
     4261 ggcctcgacg acgtgctggc ggcctatcgc tgggggcgcg acaacgccac gcggttcggc
     4321 gcggccccgg gggtcgcctc gatcggcggc gactcgatgg gcggcaactt cgccgccatc
     4381 atcgcccagg acctcaagcg cgcgggcgag ccgcagccgg ccctgcagct gctcatctat
     4441 ccggccgtgg acgtggccag tgaaacccag tcgatgacca cctacgccga cgcctacccg
     4501 ctgtcgcgtg acaccatgaa ctggttcatg ggtcactaca tgggtccgga cgccgatccc
     4561 gccgacccgc ggctgtcccc gatcaagacc gaggatctgt cgggcctggc cccggcgatc
     4621 gtggtgaccg ccggcttcga tccgctgacc gaccagggcg aatgctacgc caagcggctg
     4681 aaggacgccg gcgtgccggt gatctatcgc tgctacgaca gcctggccca cggctttacc
     4741 gccttcaccg gcgcgatccc cgccgccgac accgcctgcc gcgagatcgc cggcttcgtc
     4801 cggcaggcgg ccgaggggat gttgaagtag ccgacctttc cttcgcccct tacgggagaa
     4861 gggatttcgg acgtgactcc cgcagcggaa acgcctcaca agtccgagcg aagccggaga
     4921 agacaatgcg cgcgctggtc gtcgacagcc tgggtccgga ctacgccggc gtctccctca
     4981 aggacatccc cgtcccatcg cccgccgccg gcgaggtgct ggtcgcggtc aaggccgcca
     5041 gcgtcaactt ccccgacctg ttgatgacga ggggcgagta ccagctcaag ccgcccctgc
     5101 cgttcgtcgg cggcatggag ctggccggcg agatcgtcgc cctgggcgag ggcgtcgagg
     5161 ctttcgcggt cggcgacgcg gtggtcggcg gggccaagct gggcgggttc gccgcgttcg
     5221 ccaccctgcc cgcggcgacc atccgccgaa agccggacaa cctgaccttt tcgcaggccg
     5281 cggccatcgg cgccgcctat ctgaccgcct gggtggccct ggtgcgccgg gcgcgcatcg
     5341 aacccggcga atgggtgctg gtgcacgggg ccgccggcgg ggtcggtctg gccgccgtcg
     5401 acctggccag ggccctgggc gcccgggtca tcgcggcctc ggcctccgac gagaagctga
     5461 agatcgtcgc cgacgcgtac gcgcccgacg ctgtggtcaa tgtgaccggc ggctttcgcg
     5521 acaaggtcag ggcgatcacc ggcggggccg aggccgacgt gatctacgac ccggtcggcg
     5581 gcgaggtgtt cgacgagagc gtccgctgca tcgccttcga cggccgactg ctggtcgtgg
     5641 gtttcacctc gggccggatt ccgacgatct cggtcaacat gccgctgatc aagggcttct
     5701 cggtggtcgg cgtgcgcgcc ggggaatatg gccgccagtt ccccgatcgc ggccgcgaga
     5761 acatggacgc ggtctggaag ttggccgccg agggcgtcat ccgcccgcgt gtgcacgccg
     5821 aactgccgct cgaacgctgg cgcgaggcgt tcgatctgct ggccgaccgc aaggtcgtcg
     5881 gcaaggccat cctgcggccg gatctgtagt cggacgcccc ttcgcttgac tcggccccgc
     5941 ccttccccta tccacccccg ctcatcgcga cgggtcccgc ctggcgcgcc tggacctttt
     6001 agacttcgga gctaacccgt gagcaagcgg acctttcagc cctcgcgtct cgtgcgcaag
     6061 cgccgtcacg gctaccgcac gcggatggcc accaagagcg gtcagaagat cgtcgcgcgt
     6121 cgtcgcgcca agggccgcaa gcgcctgacg gcctagtctt tgacgcgcgc gtccccatct
     6181 gcctcgggca gggggccggg acgagttgga cgagaagcca aaccttgaac gcctgaagaa
     6241 gcggcccgat ttcctcgcgg ccgcgcgggc gccgtcgtgc gcccgtggcg cggtggtcgt
     6301 gcagcgccgc gaccgcgccg atggcgaccc cgtcattcgc gccggcttca ccgcaaccaa
     6361 gcgggtcggc ggatcggtgg tgcgcaaccg cgcccgccgc cggctgcgcg aggccgcgcg
     6421 ccttctgctg ccccagaccg gacgcccg
//
LOCUS       DAORNX010000126         6396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8994_length_6396_cov_5.838669, whole genome shotgun sequence.
ACCESSION   DAORNX010000126 DAORNX010000000
VERSION     DAORNX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8994_length_6396_cov_5.838669"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..470
                     /locus_tag="PLO65_07055"
     CDS             147..470
                     /locus_tag="PLO65_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28043.1"
                     /translation="MRVAVLGLDDADFAAELATRAPPVNDLSGLDILIYGADDPEALA
                     RIGALTPALAERGALWIVSLKGRLLKVNDVEVMAAAKAVGLVDNKVCSFSDTRTALRF
                     TRRKG"
     gene            528..1421
                     /locus_tag="PLO65_07060"
     CDS             528..1421
                     /locus_tag="PLO65_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD28044.1"
                     /translation="MALRDFGILLFVCVVWAFNNIVSKIVVAQWGVPPLFYATVRFAL
                     VALVTLPWLWPRPRPTWRIVTVAMLMGAGNFALLFIGFKTASPSAASIVVQLGVPITT
                     VLSMLMLGEQVRWRRGLGMALTLAGAVVVMWHPTGMSMSFGLWLVAAAAFAGSLGAVM
                     MKQMEGIRPLQFQAWVGFSSFWPLAVLSAFTEQNQWSTAIDAGWDFLAAVVFSAIVVS
                     VLGHTFYYALIQKYEATLISPLTLMTPLFTIVMGVLITHDHFDGRMAIGAALALLGVL
                     IIALRPNRVMPLLMAIRNRAQ"
     gene            1418..2140
                     /locus_tag="PLO65_07065"
     CDS             1418..2140
                     /locus_tag="PLO65_07065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD28045.1"
                     /translation="MTLEFIDAPSPNFDARNKVPDMLVLHYTGMQTGEAALARLRDPE
                     AKVSSHYLVEEDGRIFRLVPEERRAWHAGVSFWKGEQGVNHASIGVEIVNPGHEFGYR
                     LFPDAQIAAVIALLSDIRTRWTIDDNRILGHSDVAPGRKQDPGELFPWKTLAEAGHGV
                     WAEADPAPGDPLSEGAEGVGVFALQAGLTRLGFDCAPSGQFDAQTKDIVQAFQRHWSP
                     HRWDGVADGRTRATLMAVLRAA"
     gene            2291..2685
                     /gene="rnpB"
                     /locus_tag="PLO65_07070"
     ncRNA           2291..2685
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLO65_07070"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2912..3379
                     /locus_tag="PLO65_07075"
     CDS             2912..3379
                     /locus_tag="PLO65_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRD28046.1"
                     /translation="MFLSTFEKQLDSKRRIVVPHEFRAAVGQPFDGVFCFPSIEADCL
                     EGGGKPLFDRYVALIEELPFGDGLRSALETSVYGGMARLTFDDAGRITLPPALCDMFG
                     LTDWVSVVGMGDRFQIWPREAFQAHRAAARETARTGLAQLRGAQRLRLSGAAE"
     gene            3376..4314
                     /gene="rsmH"
                     /locus_tag="PLO65_07080"
     CDS             3376..4314
                     /gene="rsmH"
                     /locus_tag="PLO65_07080"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRD28047.1"
                     /translation="MTGDPHRSVLLDEVVRVLRPGPGRIIVDGTFGAGGYARAFLDSG
                     AEVVAFDRDPTARRFAAPLEATGRFRLLEARFSEMDRHFAPSSVDGVALDIGVSSMQL
                     DEAERGFSFMRDGPLDMRMSDSGPTAADLVNTLEKDELARIIWLYGEERESRRIASFI
                     LRRRATQPFERTLDLAEVVEKALGGRRGAKTHPATKVFQALRIAVNEELAELEAGLAG
                     AEKILKTGGRLAVVTFHSLEDRIVKAFFTARSGNLPGGSRHAPPVAGGPEPTFTLPFK
                     GAMEPGEAELAVNPRARSAKLRAGVRTEAPAWRDAA"
     gene            4311..4676
                     /locus_tag="PLO65_07085"
     CDS             4311..4676
                     /locus_tag="PLO65_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HRD28048.1"
                     /translation="MISAVINHRIRGFRTVNILFSATLLVLAVGVNFAKTIAGRERNE
                     IGRVEADIGKERQRIRLLEAEVAHLEQPERLECLARGYLAMAPVAARQETSFDALTQV
                     AAHRPRTVQPVSTAAVAVP"
     gene            4673..>6396
                     /locus_tag="PLO65_07090"
     CDS             4673..>6396
                     /locus_tag="PLO65_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD28049.1"
                     /translation="MSIADLAPHRYPPVWRWIIERVWRVEHAFERAQADGRAENDTRI
                     RIFIVLAVFAVGFSVMAGGAVWSATLAHTGRSGGAIAGAPERADLVDRQGRLLAVNLV
                     HYSLSLETRDVWSKRETRQALLAALPQLTARQVDRALAAKKRAFLIGGLTPAERARVH
                     DLGLPGVIFEPEEGRVYPLGPLAAHLIGYTEKGGESLAGAERALQAEILKAGAAGKPV
                     ALSVDVRVQAALEEELAAAAGEFHPNGAVGIVTNVHTGEILALASWPDFDPNFQQRAT
                     PDQKLNRAAASVFEMGSTFKAFTVAIGLDTRVASPDSTYDARAPLKMGYRTIHDYHGT
                     NRILTLREVFNHSSNIGTALLAHAIGNDRLAHYFSAFGLTRRAQVELVESARPLTPRK
                     WDDDDIASVSFGHGINVSPLTLVQAMGAILNGGRMIPLTIRKAPPGYRPQGERVVSEA
                     TTLSMLQIMRGNVVEGTGRKADAPGLSVGGKTGTGEKYDPAVKGYSHYKQVGSFAAVF
                     PTDGPVEADRYFVLILLDEPKGGIRTGGWVAAPAVGRTIDRIAPFLGVQRHMPPASSG
                     PTLIAARAT"
BASE COUNT          933 a         2236 c         2205 g         1022 t
ORIGIN      
        1 cctgaagggg agcgcggccg aggccggcga tctggtgctg gccggcggcg ggcggttcgc
       61 gctgggcgag gcccaggcgg gcaagtgggc gcacgccatc gccaacccgc cgggccggcc
      121 tcgacaagct gggcgtgaag ccgggcatgc gcgtcgccgt cttgggcctc gacgacgcgg
      181 acttcgccgc cgagctcgcc acgcgtgcgc cgccggtgaa cgacctcagc ggcctggaca
      241 tcctgatcta cggcgccgac gatccagagg cgctcgcgcg gatcggcgcg ttgacgcccg
      301 ccctcgccga gcgcggcgcg ctgtggatcg tgtcactgaa gggcagactg ctgaaggtca
      361 acgacgtcga ggtcatggcc gccgccaagg ccgtcggcct ggtcgacaac aaggtctgct
      421 cgttctcgga tacgcgcacg gcgctgaggt tcacacggcg caagggctga acctcgccgt
      481 aggatttctc atcgctttcc gtcacgaaac gcggctaaag cccgtccatg gccctccgcg
      541 acttcggcat cctcctgttc gtctgcgtcg tctgggcgtt caacaacatc gtcagcaaga
      601 tcgtcgtggc gcagtggggc gtgccgccgc tgttctatgc gacggtccgg ttcgccttgg
      661 tggcgctggt cacgctgcca tggctgtggc cgaggcccag gccgacctgg cggatcgtca
      721 ccgtcgctat gctgatgggg gccggcaatt tcgcgctgct gttcatcggc ttcaagaccg
      781 ccagcccgtc ggcggcgtcg atcgtcgtcc agctgggcgt gccgatcacc acggtgctgt
      841 cgatgctgat gctcggcgag caggtgcgct ggcgtcgcgg cctgggcatg gcgctgaccc
      901 tggcgggcgc cgtggtggtc atgtggcatc cgaccggcat gagcatgtcg ttcggcctgt
      961 ggctggtcgc cgccgccgcc ttcgccggct cgctgggcgc ggtgatgatg aagcagatgg
     1021 agggcatcag gcccctgcag tttcaggcct gggtcgggtt ctcgtcattc tggccgctgg
     1081 ccgtgctgag cgcgttcacc gaacagaacc agtggtcgac ggccatcgac gccggctggg
     1141 acttcctggc cgccgtcgtg ttttccgcca ttgtggtctc ggtgctgggc cacaccttct
     1201 actacgcgct gatccagaag tacgaggcga cgctgatctc gccgctgacc ctcatgacgc
     1261 cgctgttcac catcgtcatg ggcgtgctga tcacccacga ccacttcgac ggacggatgg
     1321 ccatcggcgc cgccctcgcg ctactagggg tgctgatcat cgccctgagg cccaaccgcg
     1381 tgatgcccct gctcatggcc atccggaacc gcgcgcaatg acgctggagt tcatcgacgc
     1441 cccgtcgccc aacttcgacg cccgcaacaa ggtccccgac atgctggtgc tgcactacac
     1501 cggcatgcag accggcgagg cggcgctggc gcgcctccgg gacccggagg cgaaggtctc
     1561 gtcccactac ctggtcgagg aagacggccg cattttccgc ctcgtgccgg aggagcgccg
     1621 cgcctggcac gccggcgtct cgttctggaa gggcgagcag ggcgtcaacc acgcctcgat
     1681 cggcgtcgag atcgtcaatc cgggccatga gttcggctat cgcctgttcc ccgacgcgca
     1741 gatcgcggcg gtcatcgcgc ttctgagcga catccgcacc cgctggacga tcgacgacaa
     1801 tcgcatcctc ggccattcag acgtcgcgcc cggccgcaag caggatccgg gcgagctgtt
     1861 tccgtggaag acgctggccg aggccggcca cggcgtctgg gccgaagccg atcccgcgcc
     1921 gggcgacccg ctgtcggaag gcgcggaggg cgtcggcgtc ttcgcgcttc aggccgggct
     1981 cacgcgcctc ggtttcgact gcgcgccgtc gggccagttc gatgcgcaga cgaaggacat
     2041 cgtccaggcc ttccagcgcc actggtcccc gcaccgctgg gacggcgtcg ccgacggccg
     2101 aacgcgggcg acgctgatgg cggtgctgcg ggccgcgtag gggctctgcg tccttcgacg
     2161 cgccgcttcg cggcggctca ggatgacttg ctcagacggc gattctccac gtcatggtga
     2221 gcggcgccgg agacgcgcgt cgaaccacgc attgacgttc cgtccctcag cgatcatgtg
     2281 ttcgcccgga tcagacggcc gggcgatcgc ggcgcttcgg cgtcgaggaa agtccgggct
     2341 ccacggcgac aaggcggcgg gtaacgcccg cccggggtga cccgagggat agcgccacag
     2401 aaagcaaacc tcctccgctt cggcggcggg aagggtgaaa gggtggggta agagcccacc
     2461 gcggacgtgg cgacacggac ggcatggcaa gccccgcctg gagcaagacc gaatagggat
     2521 tccgcgcccg gtgtgtccac ggatactgcc gggcagggcc gtttccggcc cgaggcgtcc
     2581 gggttggtcg cgagaaccgt ccggcaacgg gcggtccaga ggaatgatcg tcgcgcgccc
     2641 gcaagggcgt tggcacagaa cccggcttac aggccgtctg atccgcccct gcccatcagg
     2701 cctgacaccg gctgtccggg ttatccccgc tgttattctc gtgttcagtg tatgttctgt
     2761 gaatagttta cagggattaa ccggtccccc tgcgacatag ttgtctttat gaagaaatgg
     2821 ccaaggtgtc attgcgtccc atttcatccc atgttaaccc aactgttgtc gccggaaggg
     2881 gttggcgacg tcgggccagg ggctgggcgc ggtgttcctc tcgaccttcg agaagcagct
     2941 ggacagcaag cggcgcatcg ttgtgccgca tgaatttcgc gccgccgtgg gccagccgtt
     3001 tgacggcgtg ttctgctttc cctccatcga ggccgactgt ctggaaggtg gcggaaagcc
     3061 gttgtttgat cgttacgtcg ccctgatcga ggagttgccc ttcggcgacg gcctgcggtc
     3121 ggcgctggaa accagcgtct acggcggtat ggcccgtctg accttcgacg acgcgggacg
     3181 gatcaccctg ccgccggccc tgtgcgacat gttcggcctg accgactggg tgtcggtcgt
     3241 tggcatgggc gaccggttcc agatctggcc gcgagaggcc ttccaggccc accgcgcggc
     3301 cgcccgcgag accgcccgca ccggcctggc gcagctgcgc ggggcccagc gcctgcgcct
     3361 gtcgggggcg gccgaatgac cggcgatccc catcgctccg tcctgctcga cgaggtcgtc
     3421 agggtgctgc ggcccggacc gggccggatc atcgtggacg gcaccttcgg cgcgggcggc
     3481 tatgcccgcg cctttctcga ttccggggcc gaggtcgtgg ccttcgaccg cgacccgacc
     3541 gcgcggcggt tcgccgcgcc cctcgaggcc accggccgct tccggctgct ggaggcgcgc
     3601 ttctcggaga tggaccggca tttcgcgcca tcgtcggtcg atggcgtcgc gctcgacatc
     3661 ggcgtgtcct cgatgcagct ggacgaggcc gagcgcggct tttccttcat gcgcgacggt
     3721 ccgctggaca tgcgcatgag cgacagcggg ccgaccgcgg ccgacctcgt caacacgctg
     3781 gagaaggacg agctggcccg gatcatctgg ctctatggcg aggagcgcga gagccgccgg
     3841 atcgcctcct tcatcctgcg ccgccgcgcg acacagccgt tcgaacggac gctggacctg
     3901 gccgaggtgg tcgagaaggc cctcggcggg cgtcgcgggg ccaagaccca cccggcgacc
     3961 aaggtgttcc aggccttgcg catcgcggtc aacgaggaac tggccgagct cgaggcgggc
     4021 ctggccggcg ccgaaaagat cctcaaaacg ggcggccggc tcgcggtcgt aaccttccat
     4081 tcgctagaag accgcatcgt gaaggccttc ttcaccgcgc gctccggcaa cctgcccggc
     4141 ggttcgcgtc acgccccgcc ggtggcgggc gggcccgagc cgaccttcac cctgccgttc
     4201 aagggcgcga tggagcccgg cgaggcggag ctggcggtca atccgcgcgc ccgctccgcc
     4261 aagttgcgcg ccggcgtgcg caccgaagcg ccggcctgga gggacgccgc gtgatcagcg
     4321 ccgtgatcaa ccatcgcatc cgcggctttc gcaccgtgaa catcctgttc tccgcgacgc
     4381 tcctggtgct cgccgtgggg gtcaatttcg ccaagaccat cgccggccgc gaacgcaacg
     4441 aaatcggccg ggtggaggcc gacatcggca aggaacgcca gcgcatccgg ctgctcgagg
     4501 ccgaggtggc gcacctggag cagcccgagc ggctggagtg cctcgcgcgc ggctacctgg
     4561 ccatggcccc ggtggcggcg cggcaggaga cgtccttcga tgccctgacc caggtcgcgg
     4621 cccaccggcc gagaaccgtg cagccggttt cgaccgcggc ggtggctgtc ccatgagcat
     4681 cgccgacctc gccccgcatc ggtatccgcc ggtctggcgc tggatcatcg aacgcgtctg
     4741 gcgggtcgag cacgccttcg agcgcgccca ggccgatggc cgggccgaga atgacacccg
     4801 catccggatc ttcatcgtgc tggcggtctt cgccgtcggg ttctccgtca tggccggcgg
     4861 cgccgtctgg tcggccacgc tggcccacac cggccggtcc ggcggcgcga tcgcgggcgc
     4921 ccccgagcgc gccgacctgg ttgaccggca aggccggttg ctggcggtca acctggtgca
     4981 ctattcgctg tcgctcgaga cgcgggacgt gtggagcaag cgcgagaccc gccaggccct
     5041 gctggccgcc ctgccgcagc tgaccgcccg gcaggtcgac cgggcgctgg ccgcgaagaa
     5101 gcgcgccttc ctgatcggcg gcctgacccc cgcggagcgc gcccgggtgc atgacctggg
     5161 gctgcccggc gtgatcttcg aaccggagga ggggcgggtc tatccgctcg gtccgctggc
     5221 ggcccatctg atcggctaca ccgaaaaggg tggcgaaagc ctcgccgggg ccgaacgggc
     5281 cctgcaggcg gaaatcctca aggccggcgc ggcgggcaag cccgtcgccc tgtcggtcga
     5341 tgtgcgcgtc caggcggccc tggaagagga actggcggcg gcggccggtg aattccaccc
     5401 caacggcgcc gtggggatcg tcaccaacgt tcacaccggc gaaatcctgg ccctcgccag
     5461 ctggcccgac ttcgatccca acttccagca gcgggcgacg ccggaccaga agctgaaccg
     5521 ggcggcggcc tcggtcttcg agatgggctc gacgttcaag gccttcaccg tggccatcgg
     5581 cctggacacc cgcgtggcca gcccggattc gacctacgac gcccgggcgc cgctgaagat
     5641 gggctatcgc accatccacg actatcacgg caccaatcgc atcctgaccc tgcgcgaggt
     5701 gttcaaccac tcctcgaaca tcggcaccgc cctgctggcg cacgccatcg gcaatgaccg
     5761 cctggcccac tatttctcgg ccttcggcct tacgcgccgc gcccaggtgg agctcgtcga
     5821 atccgcccgg ccgctgacgc cgcgcaagtg ggacgatgac gacatcgcca gcgtctcgtt
     5881 cggccacggc atcaacgtgt caccgctgac cctggtccag gcgatgggcg ccatcctcaa
     5941 tggcgggcgg atgatccccc tgacgatccg caaggcgccc cccggctatc ggccgcaggg
     6001 agagcgcgtg gtctccgagg cgaccaccct gtcgatgctg cagatcatgc gcggcaacgt
     6061 ggtcgagggc actggccgca aggccgacgc gcccggcctg tcggtcggcg gcaagaccgg
     6121 caccggcgag aagtacgatc cggcggtcaa gggctacagc cactacaaac aggtgggctc
     6181 cttcgccgcg gtgtttccga ccgacggacc ggtcgaggcc gaccggtact tcgtgttgat
     6241 cctgctggac gaacccaagg gcggcatccg caccggcggt tgggtcgcgg ctccggccgt
     6301 cggccgcacg atcgaccgca tcgccccctt cctcggcgtg cagcgccaca tgcccccggc
     6361 gtcgagcggg ccgaccctga tcgccgcccg cgccac
//
LOCUS       DAORNX010000127         6395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8996_length_6395_cov_8.301577, whole genome shotgun sequence.
ACCESSION   DAORNX010000127 DAORNX010000000
VERSION     DAORNX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8996_length_6395_cov_8.301577"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLO65_07095"
     CDS             <1..224
                     /locus_tag="PLO65_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRD28050.1"
                     /translation="TSVDPGDSRVRRVVLTPAGHARLAAALPIWTRTHAAVEAGLADG
                     DPDRLRGDLLTLAAAKPDAATPPSCSVPE"
     gene            229..879
                     /locus_tag="PLO65_07100"
     CDS             229..879
                     /locus_tag="PLO65_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD28051.1"
                     /translation="MTATVTAFKWVPPFAQGFVRDLRIRWALEEAGLPYAVELIGPET
                     TASEAYRQWQPFGQVPAWRDDEVEIFESGAILIHLAGKSEALAPQDPAGLARVTTWIV
                     SGLNSVEPFAQNLATLDSFHAGQPWVEGYRPVARGALEKRLTSLSKWLGDKDWLEGRF
                     TAADIIMVTVLRELVACGILKDFPRLDAYRQRGEDRPAFRKAMADQLATFREHEPA"
     gene            918..1259
                     /locus_tag="PLO65_07105"
     CDS             918..1259
                     /locus_tag="PLO65_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD28052.1"
                     /translation="MKTFLALYMGSMSADAASPADLSEETIAEGMAAWGAWMARHAAV
                     IVDTGGPLGKTLKAAKDGVTETRNAVAGYVIVKAESHADAAALFKGHPHFSIFPGESV
                     EVMERLPIPGS"
     gene            1325..1582
                     /locus_tag="PLO65_07110"
     CDS             1325..1582
                     /locus_tag="PLO65_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRD28053.1"
                     /translation="MKHWPVQDAKARFSELLRASLSEGPQMVTLRGQDAAVLVPVEEW
                     KRLQRSARPSLKDILLGDGPRFDLELEPRPTLTPRPVDLDD"
     gene            1588..1992
                     /locus_tag="PLO65_07115"
     CDS             1588..1992
                     /locus_tag="PLO65_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD28054.1"
                     /translation="MWLLDTNIVSEMRKARPHGGVLAWMSRHEQVDFFISAATVGEIQ
                     VGIERSRRANPAKAVEIEAWLDTFADDDRILVADGAVFRQWAKLMSGRSPGLTMDAMI
                     AATAIVHRLTVATRNVRDFETLGLKVENPFDG"
     gene            complement(1989..3341)
                     /gene="glmM"
                     /locus_tag="PLO65_07120"
     CDS             complement(1989..3341)
                     /gene="glmM"
                     /locus_tag="PLO65_07120"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRD28055.1"
                     /translation="MSKRTYFGTDGIRGQANSHPMTAEVALRVGLAAGKLFRRNHGRR
                     HLVVIGKDTRLSGYMIEPALVAGFTSMGMDVRLFGPLPTPAVAMMTRSMRADLGVMIS
                     ASHNAYTDNGIKLFGPDGYKLSDARELEIEAHMDEGLQEGLVAPGDLGRVRRVDDAQA
                     RYVEIAKASFPRQLNLNGLRVVIDCANGAAYKVAPEVLYELGAEVIPVGVSPNGANIN
                     ADCGSTHPAAMIAAVREYRADIGIALDGDADRVAICDETGRLVDGDQIMAIIAGAWAA
                     EDRLSGGGVVATVMSNLGLERFLRGRGLTLERTPVGDRYVMERMRGGGFNLGGEQSGH
                     VILSDYSTTGDGLMAGLQVLAVLVQAGRPMSALARQFEPVPQRMENVRFAKGGKPLDS
                     DRVKAAIAEAETRLNGSGRVLVRASGTEPLIRVMAEGDDEALVAALVSEIAGAVKAAA
                     "
     gene            3479..5299
                     /gene="glmS"
                     /locus_tag="PLO65_07125"
     CDS             3479..5299
                     /gene="glmS"
                     /locus_tag="PLO65_07125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRD28056.1"
                     /translation="MCGIIGIVGKAPVTDRILESLKRLEYRGYDSAGVAVQHGGLVQR
                     RRAQGKIRALEAVLAEQPLTATVGIGHTRWATHGAPSERNAHPQTGGRVTLVHNGIIE
                     NFAELKAELQAAGRTFESDTDTEVIAQLIDSCLAAGQAPLAAFKSSLDRLTGAYALAV
                     LIEGEDNFIMGARRGSPLVVGTGDGEMFLGSDALAVGPFTNRVTYLEEGDYVAIDHDR
                     AEIFDERGTPVDRPMKIVSASAALVEKGNYRHFMEKEIHDQPEGCQRTIAAYIDAVAG
                     RTAMPGGVDLAALERIQIVACGTSYIAGVVGKYLIEKLADLPVDVEIASEFRYREPAL
                     RPGALAIAMSQSGETADTLAALRHCKARGMKTAAVVNAQESTMAREVDVVWPIHCGPE
                     IGVASTKAFTAQVSVLTALAVAAARARGRIDADEEQRLVRVLLEAPRLIAESIGLEEA
                     VRTVAVDVAKARDVLYLGRGPMSALALEGALKLKEISYIHAEGYAAGELKHGPIALVD
                     EHMPIIILAPFDSYFEKSASNMSEVMARGGQVVFITDPDGAKHAPAGAKVVMTAPASD
                     PLISALVMAAPIQLLAYHVAVLKGADVDQPRNLAKSVTVE"
     gene            5311..6177
                     /locus_tag="PLO65_07130"
     CDS             5311..6177
                     /locus_tag="PLO65_07130"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRD28057.1"
                     /translation="MKPVRKAVLPVAGLGTRVLPGAKTTPKEMLNVVDRPILSYIIAE
                     GRAAGIEHFVFVTGRGKGAIEDYFDHHIELESQLEAKGKLDMLADIRGELPRPGEMSF
                     VRQMAPLGLGHAVWCARDVIGDEPFAVMLPDMLMDAGVPALKQAVEAYNTVGGNIIAV
                     EPCPPGEAHKYGIVALEAGDGRLNRMTGMVEKPAPGTEPSNLFISGRYILQPEIFSLL
                     AAQQKGAGGEIQLTDAMARLMAVESFHALEYDGTTYDCGDKIGLLRANVAFALKRPDL
                     ADAARAAIEKLL"
     gene            6221..>6395
                     /locus_tag="PLO65_07135"
     CDS             6221..>6395
                     /locus_tag="PLO65_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD28058.1"
                     /translation="MNHLLVFGYGFTGQALARRLRGEGWTVTAVVRTPETAAKARAQG
                     VAPIEVADRAGLVE"
BASE COUNT         1041 a         2243 c         2151 g          960 t
ORIGIN      
        1 agaccagcgt cgaccccggc gacagccggg tgcgccgggt cgtcctgacc ccggccggcc
       61 acgcgcggct ggcggccgcc ctgccgatct ggacccgcac ccacgctgcg gtggaggccg
      121 ggctcgccga tggcgatccc gaccgtctgc gcggcgacct gctgaccctg gccgcggcga
      181 agcccgacgc ggccacgccg ccttcctgta gcgtcccgga gtagactcat gaccgccacc
      241 gtcaccgctt tcaaatgggt tccgcccttc gcccagggct tcgtgcgcga cctgcgcatc
      301 cgctgggcgc tggaggaagc cggcctgccc tacgcggtcg aactgatcgg gccggagacc
      361 acggcgtcgg aggcctatcg ccagtggcag ccgttcggcc aggtgccggc ctggcgggac
      421 gacgaggtcg agattttcga gtccggcgcg atcctgatcc acctcgccgg caagtcggag
      481 gccctggcgc cgcaggatcc ggcggggctg gcgcgggtga ccacctggat cgtctcgggc
      541 ctgaactcgg tcgagccgtt cgcccagaac ctggcgacgc ttgattcctt ccatgcgggc
      601 cagccctggg tcgaaggcta ccggccggtg gcgcgaggcg ccctggagaa gcggctgacc
      661 tccctgagca agtggctcgg cgacaaggac tggctggagg gccggttcac cgccgccgac
      721 atcatcatgg tcaccgtgct gcgcgaactg gtcgcctgcg gaatcctgaa ggatttcccc
      781 cgcctcgacg cctaccgcca gcgcggcgag gaccgcccgg cctttcgcaa ggcgatggcc
      841 gaccagctgg cgacgttccg cgaacacgag ccggcctgac ggcccaccgg ccggcaaacg
      901 cacacaagga gacagccatg aagacgtttc tcgccctgta catgggttcg atgtccgccg
      961 acgcggcctc gccggccgat ctcagcgaag agaccatcgc cgagggcatg gccgcctggg
     1021 gcgcctggat ggcccggcac gccgccgtga tcgtcgacac cggcggcccc ctgggcaaga
     1081 ccctgaaggc cgcgaaggac ggcgtgaccg aaacccgtaa cgccgtggcc ggctacgtca
     1141 tcgtcaaggc cgaaagccac gccgacgccg cggccctgtt caagggtcac ccgcacttct
     1201 cgatcttccc cggagagtcc gtggaagtga tggagcggtt gccgatcccg ggaagctgac
     1261 gcctcgatcg tgcttgctgg ccagcgcaat actgactagt tatctgacta gtcaggagat
     1321 catcatgaag cactggcccg ttcaggacgc caaggctcgt ttcagcgaac tgctgcgcgc
     1381 cagcctcagc gagggtccgc agatggtcac cttgcgtggc caggacgcgg ccgtgctggt
     1441 gcctgtcgag gagtggaagc gcctgcaaag gagcgcccgg ccctcgctga aagacatcct
     1501 actgggcgac ggtcctcggt ttgaccttga gctcgaaccc cgccccaccc tgacgccccg
     1561 cccggtcgat ctggacgact gaccatcgtg tggttgctgg acaccaatat cgtctcggaa
     1621 atgcgcaagg cacgaccgca tggcggcgtg ctggcgtgga tgagccgcca cgagcaggtg
     1681 gacttcttca tctcagcagc gacggtcggc gaaatccagg tcggtattga acgctctcga
     1741 cgcgccaatc ccgcaaaggc cgtcgagata gaagcctggc tggacacatt cgccgacgac
     1801 gaccgcattc ttgtcgctga cggcgccgtc tttcgtcagt gggccaaact catgagtggt
     1861 cgatccccgg gactgaccat ggacgcgatg atcgcggcga cagccatcgt tcaccgccta
     1921 acggtcgcca cccgaaacgt gcgcgatttc gaaacgcttg gcctgaaggt cgagaacccg
     1981 ttcgacggtt aggccgccgc cttcaccgcc ccggcgatct cgctgaccag ggcggcgacg
     2041 agcgcctcgt cgtcgccttc ggccatcacc cggatcagcg gctcggtgcc cgaggcgcgg
     2101 accagcacgc ggcccgagcc gttcagcctg gtctcggcct cggctatggc ggccttgacc
     2161 ctgtcgctgt cgagcggctt gccgcccttg gcgaaacgga cgttctccat ccgctggggc
     2221 accggctcga actgccgcgc cagggcgctc atcggcctcc ccgcctggac cagcaccgcc
     2281 aagacctgca ggccggccat cagaccgtcg ccggtggtcg agtagtccga caggatgaca
     2341 tggcccgact gctcgccgcc gagattgaag ccgccgccac gcatccgctc catcacgtag
     2401 cgatcgccga ccggcgtgcg ctccagcgtc aggccgcgcc cccgcaggaa ccgctcgagc
     2461 ccgagattgg acatcaccgt ggcgaccacc ccgccgccgc tgagccggtc ctcggccgcc
     2521 caggcgccgg cgatgatggc catgatctgg tcgccgtcga ccagccggcc tgtctcgtcg
     2581 cagatcgcca cccggtcggc atcaccgtcc agggcgatgc cgatgtcggc gcgatactcg
     2641 cgcaccgcgg cgatcatcgc cgcaggatgg gttgagccgc agtcggcatt gatgttggcc
     2701 ccgttggggc tgacccccac cgggatcact tcggcgccca gttcgtagag cacctcgggc
     2761 gcgaccttgt aggcggcgcc gttggcgcaa tcgatgacca cgcgcaggcc gttgaggttc
     2821 agctggcgcg gaaagctggc cttggcgatc tcgacatagc gggcctgggc gtcatcgacg
     2881 cgccgcacgc ggcccaggtc gccgggcgcg accagaccct cctgcaggcc ctcgtccata
     2941 tgggcctcga tctccagctc gcgcgcgtcg ctgagcttgt agccgtcggg cccgaacagc
     3001 ttgatgccgt tgtcggtgta ggcgttgtgc gaggcgctga tcatgacgcc gaggtcggcc
     3061 cgcatcgatc gggtcatcat cgccaccgcg ggggtcggca gcgggccgaa cagccgcacg
     3121 tccatgccca tgctggtgaa gccggcgacc agggccggct cgatcatgta gcccgaaagg
     3181 cgggtgtcct tgccgatgac caccagatgg cggcggccgt ggttgcggcg gaacagcttg
     3241 ccggcggcca gccccacgcg cagggcgacc tcggcggtca tcgggtggct gttggcctgg
     3301 ccgcgaatgc cgtcggtgcc gaaataggtt cgcttgctca tggaagtccc cggtctcctg
     3361 ggccgcaata gtctgcaacg cggttttatg cggcccgcag gtcagccgat gttaaacgcc
     3421 gacacgataa aaattctcct gacttgatat cccttcggac ggaaacggcg aaaccggcat
     3481 gtgcggcatc atcggcatcg tgggcaaggc gcccgtcacg gaccgcattc tcgaaagcct
     3541 caagcggctc gagtatcgcg gttacgactc ggccggcgtg gccgttcagc acggcgggct
     3601 ggtgcagcgc cgacgcgccc agggcaagat ccgcgcgctg gaagccgtgc tggccgagca
     3661 gccgctgacg gccaccgtcg gcatcggcca tacccgctgg gcgacccacg gcgcgccgtc
     3721 cgagcgtaac gcccaccccc agacgggcgg acgggtgacc ctggtgcaca acggcatcat
     3781 cgagaacttc gccgagctga aggccgagct ccaggccgcc ggccggacgt tcgaaagcga
     3841 caccgacacc gaggtcatcg cccagctgat cgattcctgc ctggccgcgg gccaggcccc
     3901 gctggcggcg ttcaagtcgt cgctcgaccg cctgaccggc gcctacgccc tggccgtgct
     3961 gatcgagggc gaggacaatt tcatcatggg cgcccggcgc ggcagcccgc tggtcgtcgg
     4021 tacgggcgac ggcgagatgt tcctcgggtc agacgccctg gcggtcgggc cgttcaccaa
     4081 ccgggtgacc tatctggaag agggcgacta tgtcgccatc gaccacgacc gggccgagat
     4141 cttcgacgag cgcggcacgc cggtcgaccg gccgatgaag atcgtctccg cctccgccgc
     4201 cctggtcgag aagggcaact accggcactt catggaaaag gagatccatg accagccgga
     4261 gggctgccag cgcacgatcg ccgcctatat cgacgcggtg gcgggccgta cggcgatgcc
     4321 cggcggcgtc gacctggccg cgctggagcg gatccagatc gtcgcctgcg gcacctccta
     4381 catcgccggg gtggtcggca agtatctgat cgagaaactc gccgacctgc cggtggacgt
     4441 ggagatcgcc tcggagttcc gctaccgcga accggccttg cgcccgggcg ccctggcgat
     4501 cgccatgtcg cagtcgggcg agaccgcaga caccctggcc gccctgcgcc actgcaaggc
     4561 caggggcatg aagacggcgg ccgtggtcaa cgcgcaggaa tcgaccatgg cccgcgaggt
     4621 cgacgtggtc tggccgatcc actgcggccc ggagatcggc gtggcctcga ccaaggcctt
     4681 caccgcccag gtcagcgtgc tgaccgccct ggccgtggcc gccgcccggg cgcggggccg
     4741 gatcgacgcc gacgaggagc agaggctggt gcgggtgctg ctcgaggcgc cccggctgat
     4801 cgccgagtca atcgggctgg aagaggcggt gcggaccgtg gccgtcgacg tggccaaggc
     4861 tcgcgacgtg ctctatctcg gtcgcgggcc aatgtcggcg ttggctttgg aaggcgccct
     4921 gaagctcaag gaaatcagct atatccatgc agaaggttat gctgcgggcg aactgaagca
     4981 cggaccgatc gcgctggtcg acgagcacat gccgatcatc atcctggcgc cgttcgacag
     5041 ctatttcgaa aagtcggcca gcaacatgag cgaggtgatg gcccgcggcg gccaggtggt
     5101 gttcatcacc gaccccgacg gcgcgaaaca cgcccccgcc ggagccaagg tggtgatgac
     5161 cgcccccgcc agcgacccgc tgatttccgc cctcgtcatg gccgcgccaa tccagttgct
     5221 cgcctatcac gtagctgtcc tgaaaggggc cgatgtcgat cagccccgca acctcgccaa
     5281 atccgtgacc gtcgagtaga cctcccgccc atgaaacccg ttcgcaaagc cgtcctcccc
     5341 gtcgccggcc tcggcacccg cgtgctgccc ggcgccaaga ccacgcccaa ggagatgctc
     5401 aacgtcgtcg accggccgat cctgtcctac atcatcgccg agggccgggc cgccgggatc
     5461 gagcacttcg tgttcgtcac cggccgcggc aagggcgcca tcgaggacta tttcgatcac
     5521 cacatcgagc tggagagcca gctggaggcc aagggcaagc tcgacatgct ggccgacatc
     5581 cggggcgagc tgccccgccc gggcgagatg agcttcgtgc gccagatggc cccgctgggc
     5641 ctgggccacg ccgtctggtg cgcccgcgac gtgatcggcg acgagccgtt cgccgtcatg
     5701 ctgcccgaca tgctgatgga cgccggggtt ccggccctga agcaggcggt cgaggcctac
     5761 aacacggtcg gcggcaacat catcgccgtc gagccctgcc cccccggcga ggcgcacaag
     5821 tacggcatcg tggccctcga ggccggcgac ggccgcctga accgcatgac cggcatggtc
     5881 gagaagccgg ccccgggaac cgaaccctcg aacctgttca tttccggccg ctacatcctg
     5941 cagcccgaga tcttcagctt gctggccgcc cagcagaagg gcgccggcgg agagatccag
     6001 ctgaccgacg ccatggcccg gttgatggcc gtcgagagct tccacgcgct ggaatacgac
     6061 ggaacaacct acgactgcgg cgacaagatc ggcctgctcc gggccaatgt ggcgttcgcg
     6121 ctgaagcggc cggacctggc cgacgcggcg cgggcggcga tcgaaaagct gctgtgagcg
     6181 cctgtccttc tcccggcggg ggaaggtggg aggggggtcg atgaaccacc tcctcgtctt
     6241 cggctacggc ttcaccggcc aggcgctggc ccggcggctg cggggcgaag gctggaccgt
     6301 cacggccgtt gtccgcacgc cggaaaccgc cgccaaggcc cgcgcccagg gcgtcgcgcc
     6361 gatcgaggtg gccgaccggg cgggcctggt cgagg
//
LOCUS       DAORNX010000128         6395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_8997_length_6395_cov_7.093849, whole genome shotgun sequence.
ACCESSION   DAORNX010000128 DAORNX010000000
VERSION     DAORNX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8997_length_6395_cov_7.093849"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLO65_07140"
     CDS             complement(<1..906)
                     /locus_tag="PLO65_07140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD28059.1"
                     /translation="MTFLSVNPAIDRALVDQGYLEPTPVQLAVLEAEAEGRDLLVSAQ
                     TGSGKTVAFGMAMAPTLLGEAERFGETGAPVALIIAPTRELALQVSRELTWLYARTGA
                     VVVSCVGGMDARSEQRALNRGCHIVVGTPGRLRDHLERGHLDLSALKVAVLDEADEML
                     DLGFREDLEFILEATPEDRRTLLFSATIARDIAALARTYQRDAVRIDTTTRNAPHGDI
                     EYRAIRIAPNEIEHAVVNVLRHFDAGGALVFCATRDAVRHLHSSLRERGFSVVGLSGE
                     MGQRERNDALQSLRDRHARVCVATDV"
     gene            1547..2452
                     /gene="tcmP"
                     /locus_tag="PLO65_07145"
     CDS             1547..2452
                     /gene="tcmP"
                     /locus_tag="PLO65_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HRD28060.1"
                     /translation="MESIGDHEFGSGLTDLKLSVVAEYLHRYNMALSKQPFFQRWYFD
                     AFAGTGERTVRLSGSSGDLISEPVEGRIERRRGSARIALDTTPQFHRCIFMEQKKRHA
                     VALETLCSEAPYSSRETFVVRGDANRAIQDHIQTVNWKKTRAVMFLDPYGMNVEWRTL
                     EAIRRTEAIDLWYLVSLSGLYRQATHKGSALDEKKRAALTRMLGTSDWENAWYEEDRQ
                     RSLLSDVEPEATRRTASLDRMEAFVLQRLRSLFPMVLEPLRLKNDRNAPMFSLFFAMS
                     NPSPAAAKVARPIAQHILVQARTKR"
     gene            complement(2449..3195)
                     /locus_tag="PLO65_07150"
     CDS             complement(2449..3195)
                     /locus_tag="PLO65_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HRD28061.1"
                     /translation="MAETSIEWTDATWNPVAGCTILTAGCTNCYAMRMAARLEAMGVP
                     KYAGLTRKSGERYLWTGAISLDEATLDAPKAWRKPRLIFVNSMSDLFQEAVPVEFIRR
                     VWDTMAKTPQHTYQVLTKRPDRMARITPDLPLLPNVWLGTSVENAAVLDRLDHLRATP
                     AAVRFVSFEPLIGSVAGGDLQGIHWAIIGGESGPRARPMLEEWVDEIHEMAEDAGVTF
                     FFKQWGGRNKKAAGRTYRGRHWDALPTVTA"
     gene            complement(3461..5296)
                     /gene="typA"
                     /locus_tag="PLO65_07155"
     CDS             complement(3461..5296)
                     /gene="typA"
                     /locus_tag="PLO65_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRD28062.1"
                     /translation="MSLRNIAIIAHVDHGKTTLVDQLLAQSGVFRANEATTERAMDSN
                     DQERERGITILAKCTSVLWHGEAGDTRINIIDTPGHADFGGEVERILGMVDGCVLLVD
                     AEEGVMPQTKFVLGKALKMGLKPIVCLNKVDRPHADPDRVLNDAFDLFAIMGATDEQL
                     DFPHIYASGKNGWATLDMARPNDTLAPLFDLIVKHVPSPKAEARKAEPFQMLSVLIES
                     DPFLGRLLTGRIEAGKAVPGMAIKALSRDGKEIERGRITKVLAFRGLKRQPVDEGAEA
                     GDIVAIAGLSKATVADTLCALEVSEALPAQPIDPPTISMTVSVNDSPLAGREGDKVQS
                     RVIRDRLLKEAESNVAIKVSETAERDAYEVAGRGELQLGVLIESMRREGFEVSISRPR
                     VVFQTDPETGARLEPMEDIVIDVDDEFSGIVIEKLSARKAELKDMGPSGGGKTRLQLV
                     APSRSLIGYQGEFLTDTRGSGVLNRVFSHYEPYKGPIEQQRKGVLISNSDGETAAYAL
                     WNLEERGVMFVGGGEKTYQGMIIGENARSDDLDVNPMKAKQLTNVRASGKDEAIRLTP
                     PRRPTLEQAIAYIQEDELVEVTPKNIRLRKKVLNPSFRKRRVKDE"
     gene            complement(5715..6176)
                     /locus_tag="PLO65_07160"
     CDS             complement(5715..6176)
                     /locus_tag="PLO65_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28063.1"
                     /translation="MKSIAAVIALALLLSTRAEASELKGPGRFCGYSPIIDLLSGEKI
                     TTLEGGIHGGKFRWEGSFGVLEVYGIGWASRPTGTLIEVRKGRRPARFAERSVKDRYE
                     VAIWNGAHGAAYISSATPFSRDQLKAVRRVRLFEEGQIPSGCDLRTVFSWE"
     gene            complement(6224..>6395)
                     /locus_tag="PLO65_07165"
     CDS             complement(6224..>6395)
                     /locus_tag="PLO65_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRD28064.1"
                     /translation="YGRPASRTHALQIGISRALYLDETTLEPTAGLATLTADIGRLAL
                     ALASAWPALRPA"
BASE COUNT         1054 a         2046 c         2139 g         1156 t
ORIGIN      
        1 cacgtcggtg gcgacgcaga cgcgggcgtg gcggtcgcgc agcgactgca gggcgtcgtt
       61 gcgctcccgc tggcccatct cgcccgacag cccgaccacc gagaagccgc gctcgcgcag
      121 gctggagtgc aggtggcgca ccgcgtcgcg ggtggcgcag aacaccagcg ccccgccggc
      181 gtcgaagtgg cgcagcacat tgaccaccgc atgctcgatc tcgttcggcg cgatgcggat
      241 ggcgcggtac tcgatgtcgc cgtgcggcgc gttgcgggtg gtggtgtcga tgcgcacggc
      301 gtcccgctgg taggtgcggg ccagggcggc gatgtcccgg gcgatggtcg ccgagaacag
      361 cagcgtccgc cgatcttccg gcgtcgcctc gaggatgaac tcgaggtcct cgcggaagcc
      421 gagatcgagc atctcgtcgg cctcgtcgag caccgcgacc ttgagggccg acaggtcgag
      481 gtggccgcgc tcgaggtggt cgcgcaggcg gcccggcgta ccgaccacga tgtggcagcc
      541 gcggttcagc gcgcgctgct cgctgcgggc gtccatgccg ccgacgcagg agacgacgac
      601 cgcgccggtg cgggcgtaga gccaggtcag ctcgcggctg acctgcaggg ccagctcgcg
      661 ggtcggggcg atgatcaggg ccaccggcgc gccggtctcg ccgaaccgct cggcctcgcc
      721 cagcagggtc ggggccatgg ccatgccgaa ggccacggtc ttgcccgagc cggtctgggc
      781 cgacaccagc aggtcgcgcc cctcggcctc ggcctccagc acggccagct gcacgggggt
      841 gggttcgaga tagccctggt cgacgagggc gcgatcgatc gcggggttca cggacaaaaa
      901 ggtcatttcg gggtagatcc tgacggttgc gaaaacgcgt cggatgcccc ggggtctcgc
      961 cacggcgagg ggctgtgtca gcgttttatc tgcaagtgag cgcgggctca tacaggaaga
     1021 cctgtggaaa agccagtgtg ggggctcagc gggctaccct tccccctgga ggggggaagg
     1081 tggcctgcgg cgcaggccgg atgggggtgg cggtggatgc cgccagcctg aaagacgtca
     1141 tggtgagcag cacccgcagg gtgcgtgtcg aaccacgcaa tctgttcgtg cgtccttcga
     1201 cacggccctt cgggcctgct caggatgacg tgaagcgtgg attgccccgc cgccgccacc
     1261 cccatccccg acccttcccc ccctccaggg ggaagggaga gacccttcga tctcatgcag
     1321 gccagcctgc cggacgagcc gggttggtca aggggggggg ctccgggcca tcgcctccgg
     1381 cgacgcgaag cgcccttgag caacccggat cgcccggcga tcgtctcggc atgagatgaa
     1441 gggggtggtg gggcacagag agacaaccct tgctcactta tttggccccc gcaacaaatc
     1501 agggttctct gcatgccggc gagctaaacg gatctgggag tatcgcgtgg aaagtatcgg
     1561 cgaccacgaa ttcggcagcg ggctaaccga cctcaagctc tccgtggttg cggagtacct
     1621 gcaccggtac aacatggccc tctccaaaca gcccttcttt cagcgctggt atttcgacgc
     1681 atttgccgga acgggcgagc gaacggttcg actttcagga tcgagcggcg acctcataag
     1741 cgagcctgta gaggggcgga tcgagcgccg acggggatcg gcgcggatcg cgctcgacac
     1801 gacgccccaa ttccaccgct gcatcttcat ggagcagaag aagcgccacg ctgttgcctt
     1861 ggagacgctc tgctctgagg ccccatactc ctcgagggag accttcgtcg tgcgcggaga
     1921 cgccaacagg gcgatccagg atcacatcca gaccgtcaac tggaagaaga cgcgtgcggt
     1981 gatgtttctc gatccgtacg ggatgaacgt tgagtggcgg acgctagaag cgatccgtcg
     2041 caccgaggcc atcgaccttt ggtacctcgt atcgctctcc ggcctttaca ggcaggcgac
     2101 ccacaagggg tccgctctgg acgagaagaa gcgtgccgcc ctgacgcgga tgctcggtac
     2161 ttcagattgg gagaacgctt ggtatgaaga agatcgtcag cggagtctcc tctcagacgt
     2221 agaacccgag gcgacgagac gaacggctag cttggatcgc atggaagcgt tcgtcctaca
     2281 gcgcctcagg agccttttcc ccatggtgct ggaaccgcta cgcctgaaga acgaccgcaa
     2341 cgcgcccatg ttctccctgt tcttcgccat gtcgaacccg agcccggcag ccgccaaagt
     2401 ggcaaggccc atcgcccaac atattctcgt gcaggcgcga acgaagcgtt aggcggtcac
     2461 cgtcgggagc gcgtcccaat gccgaccgcg gtaggtccgc ccggccgcct tcttgttccg
     2521 gccgccccat tgcttgaaga agaacgtgac gccagcgtcc tcggccatct catgtatttc
     2581 gtcgacccat tcctcgagca tcggtcgggc gcgaggcccg ctttccccgc cgatgatggc
     2641 ccagtggatt ccctgtaggt cgccgcccgc aactgaaccg attagtggct cgaacgacac
     2701 gaacctgacg gccgctggcg tagcccgcaa atggtcaagt cggtccagaa cggctgcgtt
     2761 ctcgacactc gtgccgagcc agacgtttgg gagaagtggt aagtcaggcg tgatccgcgc
     2821 catgcgatcc gggcgcttcg tgagcacctg gtaggtgtgt tgcggcgtct tggccatggt
     2881 gtcccagacc cgccgtatga attcgaccgg aacggcctct tgaaacaggt cggacattga
     2941 gttgacgaag atcaaccgag gcttacgcca agccttcggc gcgtccaagg tggcctcgtc
     3001 gagcgagatc gcgccggtcc ataagtagcg ctcaccgctc tttcgcgtca ggcccgcgta
     3061 ctttggaact cccattgcct caagccgggc ggccatgcgc atcgcgtagc agttggtgca
     3121 gccggccgtc aggatcgtgc aaccggcaac cgggttccaa gtcgcatcgg tccactcaat
     3181 agaggtctct gccatgatct ctcctcaagg cgaggcttcg ggagtttgct taattgcgca
     3241 ctaacgaatg gtgcgccgga agaggtccaa gaactaacat ctgaaagcag gccttacgaa
     3301 ggtcagcttc gaatcagagt cggtcctaga caggatcagc ggcaaatgaa gggcaggccc
     3361 ttcttgttca ccttccaaac gaaaaaggcg ggccgcatct ctgcgccccg cttcaatcgt
     3421 tagccccacg gcctccctgt ttcggaaggc cctgaaaccc ctactcgtcc ttcacgcgcc
     3481 gcttccggaa gctcgggttc agcaccttct tccgcaaccg gatgttcttc ggcgtcacct
     3541 cgaccagctc gtcttcctgg atgtaggcga tggcctgttc cagggtcggg cggcgcgggg
     3601 gggtcaggcg gatggcttcg tccttgccgc tggcgcgcac gttggtcagc tgcttggcct
     3661 tcatcgggtt gacgtcgagg tcgtcggacc gcgcgttctc gccgatgatc atgccctggt
     3721 aggtcttctc gccgccgccg acgaacatca cgccgcgctc ctcgaggttc cacagggcgt
     3781 aggccgccgt ctcgccgtcg gagttggaga tcagcacgcc cttgcgctgc tgctcgatcg
     3841 ggcccttgta gggctcgtag tgactgaaca cgcggttcag cacgcccgag ccgcgggtgt
     3901 cggtgaggaa ctcgccctgg tagccgatca ggctgcgcga gggggcgacc agctgcaggc
     3961 gggtcttgcc gccgccggag gggcccatgt ccttgagctc ggccttgcgg gcgctgagct
     4021 tctcgatgac gatgccgctg aactcgtcgt cgacgtcgat gacgatgtct tccatcggct
     4081 ccaggcgggc gccggtctcc gggtcagtct ggaacaccac gcgcgggcgg ctgatcgaga
     4141 cctcgaagcc ctcgcggcgc atgctttcga tcagcacgcc cagctgcagc tcgccgcggc
     4201 cggcgacctc gtaggcgtcg cgctcggccg tttcgctgac cttgatggcc acgttgctct
     4261 cggcctcctt gagcaggcgg tcgcggatca cccgactctg caccttgtcg ccctcgcggc
     4321 cggccagcgg gctgtcgttg accgaaacgg tcatcgagat ggtcggcggg tcgatcggct
     4381 gggcggggag cgcctcgctg acctccaggg cgcacagggt gtcggccacg gtggccttgg
     4441 aaaggccggc gatggcgacg atgtcgccgg cctcagcgcc ctcgtcgacg ggctggcgct
     4501 tgaggccgcg gaaggccagc accttggtga tgcggccgcg ctcgatctcc ttgccgtcgc
     4561 gcgacagggc ctttatggcc atgccgggaa cggccttgcc ggcctcgatg cggccggtca
     4621 gcaggcggcc gaggaacgga tcgctctcga tcagcaccga cagcatctgg aacggctcgg
     4681 ccttgcgcgc ctcggccttc gggctgggga cgtgcttgac gatcaggtcg aacagcgggg
     4741 ccagggtgtc gttcggcctg gccatgtcga gcgtggccca gccgttcttg ccgctggcgt
     4801 agatgtgcgg gaagtcgagc tgctcgtcgg tggcgcccat gatggcgaac aggtcgaagg
     4861 cgtcgttcag cacgcggtcg ggatcggcgt gcggccggtc gaccttgttg aggcagacga
     4921 tcggcttgag gcccatcttc agcgccttgc cgagcacgaa cttggtctgc ggcatgacgc
     4981 cttcctcggc gtcgaccagc agcacgcagc cgtcgaccat gccgaggatc cgctcgacct
     5041 cgccgccgaa gtcggcgtgg ccgggggtgt cgatgatgtt gatccgcgtg tcgccggcct
     5101 cgccgtgcca cagcacgctg gtgcatttgg ccaggatggt gatgccgcgc tcgcgctcct
     5161 ggtcgttgga gtccatggcg cgctcggtgg tcgcctcgtt ggcgcggaac acgcccgact
     5221 gggccagcag ctggtcgacc agggtggtct tgccatggtc gacgtgggcg atgatggcga
     5281 tattgcgcag ggacatggga cgactttttc ggaaacggac gggcgggagc ggaaggcgcg
     5341 cgcttgtacg cgagccggcc gggtttagcc accccggggg agtgacgcgg aacggggcgc
     5401 cggcaaacgg ctgtttttat tcaccaaaga gtgaattgag gcgcccggcg ctcgcgcatt
     5461 gtgcatcgca caatgaggcg ggtttggcaa gaatgcccca agggaacagg agtttccgaa
     5521 gggtcgtcac gcgagaatcg catcatctcg catgaattgt ccttgccaat tgtgcggcgc
     5581 acactaaatt ggagttgtga ggcggctccg gcccctctgc ccttcctggg cgtttcctcc
     5641 ctattgaact gcggcggctt cggccgccgc ttttttttgg cccggggcct tggggggaca
     5701 gtgcgctcaa gcgtctattc ccaagagaaa acggtccgca gatcgcagcc cgaagggatt
     5761 tgcccttcct cgaacagcct tactcgcctt acggccttca attggtcgcg gctgaaaggt
     5821 gtcgctgagc tgatataggc agccccgtgg gcgccattcc agatggcgac ttcgtagcga
     5881 tccttgaccg agcgctcggc aaacctggcc ggccttcttc ccttccgaac ttcgatcagg
     5941 gttccagtcg ggcggctcgc ccagccgatc ccgtaaacct cgagcacgcc aaagctgccc
     6001 tcccatcgaa actttccgcc atgtatgccg ccctcgagcg tcgtgatttt ctcgcccgac
     6061 aacaggtcga ttatggggga atagccgcaa aatcgcccgg gtccctttag ttcgctggct
     6121 tcagctctcg tggagagaag cagggccagc gcgatcaccg ctgcgataga cttcatgctc
     6181 gtatctccga aggtgattga accgcttttt gaagtccaaa gcctcaggcc gggcgtagcg
     6241 cgggccaggc cgacgcaagc gccagcgcca gcctgccgat gtccgccgtc agcgtcgcga
     6301 gccccgcggt gggctccagc gtcgtctcgt ccagatagag cgcccggctg atcccgatct
     6361 gcaacgcatg ggtgcggctg gccggccggc cgtag
//
LOCUS       DAORNX010000129         6392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9000_length_6392_cov_5.469149, whole genome shotgun sequence.
ACCESSION   DAORNX010000129 DAORNX010000000
VERSION     DAORNX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9000_length_6392_cov_5.469149"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLO65_07170"
     CDS             <1..818
                     /locus_tag="PLO65_07170"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD28065.1"
                     /translation="ATAPRPAGGAPGHGSAAPIFVFGLPRSGTTLVERIITSHPDVIS
                     TGEPMDFAGLVRRFAGAPTRELIDGPTLRAVPPGDLSNLGAAYVEAIRARHPGGSRFT
                     DKTPLNFLFGGLIHAALPQARMVCLRRHPVDSCLAVYRQRFTTGSAYYDWVYSLEATA
                     RFYLAFDALVAHWRATLPADRFREVTYEDVVSDQEGQSRALLDWLGLPWDDRVLAFHE
                     NAAPVSTASAVQVRRQVYASSVGRWKRYGDRLRPLTDILVAGGVLSADEVDAG"
     gene            complement(977..2779)
                     /gene="sppA"
                     /locus_tag="PLO65_07175"
     CDS             complement(977..2779)
                     /gene="sppA"
                     /locus_tag="PLO65_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRD28066.1"
                     /translation="MKQFLLTMAGVFAGLVLFFVLIPFLVIGSMIGAASAPKDTTPAQ
                     AVLELDLRGGITDQDPHNPFAAFGGSGLSVVSVIETLRHAEKDGKVKAVFVRLPEGGM
                     PPAAADELRSAFLHFRRAGKPIWAHSQGLYPSGVITSTYMLGAATDALWMQPDSSFQA
                     VGLATEDLFFKRFFDKYGVKAEYEQRMEYKNAVNPYLYSDYTAAHRESETAWMGSIYR
                     TGVLTAAIDRKKEPVAFVRMIEAGPYSAQDAAARGLIDRVGQVREASDALIARVGGKA
                     KLVDFEDYMGHALRAERTSGALGGKPVIAVIGAEGPIMTGRGGSGNPFSGDETVWSDD
                     VAESFYDAIENKDVKAIVFRVSSPGGSDTASEQILSAVKAARKAGKPVVVSMGTYAAS
                     GGYWISSQANEIVAQPTTLTGSIGVYGGKFALGEALARFGIDVRSTAVGGDYASTFGT
                     ASSFTPDQRVAFARWMDHIYDGFITRVSEGRKLSPDRVRQIAKGRVWTGVQARQLQLV
                     DQLGGYYDAIDRAKALAGVKGEVRIRHIDAAGSPFEAFEKLFGVSSTSLRTLAAAGWV
                     LGDPRAAAVLDELATARLRSSPSGAAVLAPTPVR"
     gene            complement(2904..2980)
                     /locus_tag="PLO65_07180"
     tRNA            complement(2904..2980)
                     /locus_tag="PLO65_07180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2944..2946),aa:Pro,seq:cgg)
     gene            3126..3199
                     /locus_tag="PLO65_07185"
     tRNA            3126..3199
                     /locus_tag="PLO65_07185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3158..3160,aa:Gln,seq:ttg)
     gene            complement(3185..4402)
                     /locus_tag="PLO65_07190"
     CDS             complement(3185..4402)
                     /locus_tag="PLO65_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRD28067.1"
                     /translation="MRYVDQHPHGGFRIFGIEIRVRLSWLILALLLAWSLASGAFPEI
                     YQGLPGRSYWTMAVLAVAGLGLSIILHELAHSLVARAFGLPIDRITLFLLGGVAELRE
                     EPRTPWSELLMAAAGPAFSVVFGLALGVLSRGVPPETDTLGLAAALDFLASVNLVLAA
                     FNMAPAFPMDGGRVLRAVIWLVTGNPARATRIAAAIGGFLALALILAGVALALTGQIA
                     AGLWWVLIGLFLRTAAHASVSDLLAQRLLTGVPVREVMALGAGPLPGHMTVAEFVRRQ
                     AYAPPQSLRAVVKDGVAVGVIEAADVLATPRQTWPDRTLARTCRPIGEAVAVDADADA
                     FAVFERMKRDRRLRLLVLLRDRPVGVLSLQDLMQRLELARRFETDDAPGRSTRPGMAA
                     STSGASGKNWLGN"
     gene            complement(4484..4924)
                     /locus_tag="PLO65_07195"
     CDS             complement(4484..4924)
                     /locus_tag="PLO65_07195"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRD28068.1"
                     /translation="MTTGLPVFDTTVQETNRWLARVQAMLPASSRQQAYAATRAVLHV
                     LRDRLPMDAVLGLSAQMPMLMRGLFLEGWRPDAGPTAIRTPEAFRDAVADRLPAGFPR
                     SAEAITPAVFAVLADRIDPGETDKLRRHLPESLRALWPADHPVA"
     gene            complement(5119..5733)
                     /locus_tag="PLO65_07200"
     CDS             complement(5119..5733)
                     /locus_tag="PLO65_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD28069.1"
                     /translation="MARGQVIYDEGEPPGVMYRLEAGCVRLQVNGEDGDRQIVAFLFP
                     GELFGFCLDRRSTSAEAVSEVEVTRFMTRMVLEKSVARPDVVVELINRSNALFGVLAH
                     HVEKITHLPAHERLIWFFNWLLRHADDARAPGVIELPMNYRDIADFLSLKPETLSRAL
                     KQLEDTGYLVRRGQRTVVLRQACVALPLRAGDRLAATQADSATG"
     assembly_gap    6118..6134
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6135..>6392
                     /locus_tag="PLO65_07205"
     CDS             <6135..>6392
                     /locus_tag="PLO65_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28070.1"
                     /translation="VIFAHGSGSSRLSPRNQEVAGSLCDAGLATLLFDLLTEEEADDR
                     SNVFDIDLLAGRLVSAIDWARARPDLAALPVGLFGASTGAAA"
BASE COUNT          990 a         2228 c         2232 g          925 t
ORIGIN      
        1 gggccacagc gccgcggccc gcgggcgggg cgccgggtca cggctcagcc gcgccgatct
       61 tcgtcttcgg cctgccgcgc agtggcacca ccctggtcga acggatcatt accagccacc
      121 cggacgtcat ctccacgggc gagccgatgg acttcgccgg tctcgtccgt cgcttcgccg
      181 gggcgcccac gcgggaactg atcgacggcc cgaccctgcg ggcggttcca ccgggggacc
      241 tgtcgaacct cggggccgcc tatgtcgagg cgatccgggc ccgtcatccg ggcgggagcc
      301 ggttcaccga caagacgccg ctgaacttcc tctttggcgg cctgatccac gcggccttgc
      361 cgcaggcgcg gatggtctgc cttcgccggc atccggtcga cagttgcctg gcggtctatc
      421 gccagcggtt caccacgggc tccgcctatt acgactgggt ctattcgctc gaggcgacgg
      481 cgcggttcta tctggccttc gacgccctgg tggcgcactg gcgggcgacg ctgccggccg
      541 accgcttccg tgaggtgacc tacgaggacg ttgtgtccga tcaggaaggc cagagccggg
      601 ccctgctgga ctggctgggc ctgccctggg acgaccgggt gctggccttc cacgagaacg
      661 cggccccggt gtccaccgcc agcgcggtcc aggtccgccg gcaggtctac gccagttcgg
      721 tcggccgctg gaagcgctat ggcgaccgcc tcaggccgct gaccgacatc ctggtggccg
      781 gcggcgtact ctcggccgac gaggtcgacg cgggttaggg ccagtcgaca tcgcgcgcgg
      841 gagctgccag gggctccgat tgttcccgcg aaggcgggaa cccaagcggt gtttcgggtc
      901 ctgacgcgcc gtttaggcgg ccatcagggc ttgggtcccc gccttcgcgg ggacaatcgg
      961 tgaatgccgg ttggccttag cgcaccggcg tcggggccag cacggcggcg ccggacgggc
     1021 tggaccgcag gcgcgcggtc gccagctcgt cgaggaccgc cgcggcgcgg ggatcgccca
     1081 gcacccagcc ggccgccgcg agggtccgca gcgaggtcga gctgaccccg aacagcttct
     1141 cgaaggcctc gaacggcgag ccggcggcgt cgatgtggcg gatgcgcacc tcgcccttta
     1201 cgccggccag ggccttggcc cggtcgatgg cgtcatagta gccgcccagc tggtcgacca
     1261 gctgcagctg gcgcgcctgg accccggtcc agacccgtcc cttggcgatc tgccggaccc
     1321 ggtcgggcga cagcttgcga ccctcggaaa cccgggtgat gaacccgtcg tagatatggt
     1381 ccatccaccg ggcgaacgcg acccgctggt cgggggtgaa gctgctggcc gtgccgaagg
     1441 tgctggcgta gtcgccgccg acggccgtgg accgcacgtc gatgccgaac cgggccagcg
     1501 cctcgccgag ggcgaacttg ccgccgtaga cgccgatcga gccggtcagg gtggtcggct
     1561 gggcgacgat ttcgttggcc tgggagctga tccagtagcc gccggacgcc gcataggttc
     1621 ccatgctgac gaccaccggc ttgccggcct tgcgggccgc cttgacggcc gacaggatct
     1681 gttcggaggc cgtgtccgac ccgccggggg aggaaacgcg gaagacgatc gccttcacgt
     1741 ccttgttctc gatcgcgtcg tagaacgact cggccacgtc atcggaccag acggtctcat
     1801 cgccggagaa cggattcccg gatccgccgc ggccggtcat gatcgggccc tcggcgccaa
     1861 tcaccgcgat caccggcttg ccgcccaggg cgccgctcgt ccgttcggcg cgcagggcgt
     1921 ggcccatgta gtcctcgaaa tcgaccagct tggccttgcc gccgacgcgg gcgatgaggg
     1981 cgtccgacgc ctcgcgcacc tggccgacac ggtcgatcag cccgcgggcg gcggcgtcct
     2041 gcgccgaata gggcccggcc tcgatcatcc gcacgaaggc caccggctcc ttcttgcggt
     2101 cgatcgccgc ggtgagcacg ccggtgcggt agatcgagcc catccaggcc gtctccgact
     2161 cgcggtgggc ggcggtgtag tcgctgtaga gatacggatt gacggcgttc ttgtactcca
     2221 tgcgctgctc gtactcggcc ttgacgccgt acttgtcgaa gaagcgcttg aagaacaggt
     2281 cctcggtcgc cagaccgacg gcctggaagg agctgtcggg ctgcatccac agggcgtcgg
     2341 tcgcggcgcc cagcatgtag gtcgaggtga tcaccccgga cggatacagg ccctggctgt
     2401 gagcccagat cggcttgccg gcccggcgga agtgcaggaa ggccgaacgc agctcgtcgg
     2461 ccgcggccgg cggcatgccg ccttccggca ggcgcacgaa caccgccttg accttgccgt
     2521 ccttctcggc gtgccgcagc gtttcgatca ccgagaccac cgacaggccc gatccgccga
     2581 aggcggcgaa cggattgtgc ggatcctggt cggtgatccc gccgcgcagg tcgagctcca
     2641 gaaccgcctg ggccggggtg gtgtccttcg gcgcgctggc cgcgccgatc atcgacccga
     2701 tgaccagaaa gggaatgagc acgaagaaca acaccaggcc cgcgaacacg ccggccatgg
     2761 tcaggaggaa ctgcttcatc ggggaccccg gatggaaaac tcgcccacgc ttatatgggt
     2821 ccgcgtttgc gtcaaatgaa cacggggtca cactccccgc gaaatcggct cttgagaaag
     2881 gatttcgcgg ggcgcgggaa taatggtcgg agtgaaagga ttcgaacctt cgacccccgc
     2941 ctcccgaaga cggtgctcta ccagactgag ctacactccg acgtaaggac gcggcttata
     3001 gaggcgagtc tgaagctccg caagcggcca tcagaaagcc ttcacgcgcc gttgcatcgc
     3061 gtcctagcct gcgctatctg acgcgcctcg cgacgcccgg ctccccggag ccccgccgtt
     3121 cctgctgggg aatggtgtaa tggtaacaca gcggtttttg gtaccgtcat tctaggttcg
     3181 agtcctagtt ccccagccag ttcttccccg aagcgcctga tgtcgaagcc gccatgccgg
     3241 gccgcgtcga gcggccgggc gcgtcatcgg tttcgaaccg ccgggccagc tccagtcgct
     3301 gcatcagatc ctgcagcgac agcaccccga ccggcctgtc ccgcagcaga accagcagcc
     3361 gcagccggcg gtcgcgcttc atccgctcga acacggcgaa ggcgtcggca tcggcatcca
     3421 ccgcgacggc ctcgccgatc gggcggcagg tccgggccag cgtgcgatcg ggccaggtct
     3481 ggcggggcgt ggccagcacg tcggcagcct cgatcacgcc caccgccacg ccgtccttca
     3541 cgaccgcccg cagcgactgg ggcggggcgt aggcctggcg acggacgaac tcggcgaccg
     3601 tcatatgtcc gggcagcgga ccggctccca gggccatcac ctcgcgcacc ggaacgccgg
     3661 tcagcagccg ctgcgccaga aggtccgaca ccgaggcatg ggccgccgtg cgcaggaaca
     3721 ggccgatcag cacccaccac aggccggcgg cgatctggcc ggtcagcgcc agggccacgc
     3781 cggccagtat cagggccagg gccaggaagc cgccgatggc cgcggcgatg cgggtggccc
     3841 gggccggatt gccggtgacc agccagatca cggcccgcaa cacccggccg ccgtccatgg
     3901 gaaaggcggg cgccatgttg aacgccgcca gcaccagatt gacgctggcc aggaagtcca
     3961 gcgccgccgc caggcccagg gtgtcggtct cgggcgggac cccgcgtgac aggacgccca
     4021 gcgccaggcc gaagacgacg ctgaaggccg gtccggccgc cgccatcagc agttcggacc
     4081 atggggtgcg gggctcctcg cgcagttcgg cgacgccgcc cagcaggaac agggtaatgc
     4141 ggtcgatcgg caggccgaag gcccgggcca ccaggctgtg cgccagctcg tgcaggatga
     4201 tcgacaggcc cagcccggcg acggccagca ccgccatggt ccagtaggac ctgcccggca
     4261 ggccctggta gatctccggg aaggcgcccg aggccagcga ccaggcgagc agcagggcca
     4321 ggatcagcca gctgagccgc acgcgaatct cgatgccgaa tatccggaaa cccccgtggg
     4381 gatgctgatc gacgtagcgc atgtctggtc ctcgctgacg gagcggacct tcggccgggc
     4441 ggcgctggac gtcgttgatc tttctcaacc gggccgggcg gcctcaggcc acagggtggt
     4501 cagccggcca gagcgcgcga agggactcgg gcaggtggcg ccgcagcttg tccgtctcgc
     4561 ccggatcgat gcggtcagcc agcaccgcga acacggccgg ggtgatcgcc tcggccgacc
     4621 gggggaagcc cgccggcagg cggtcggcca cggcgtcgcg gaaggcctcg ggcgtccgga
     4681 tggcggtcgg ccccgcgtcc ggacgccagc cttcgaggaa caggccgcgc attagcatcg
     4741 gcatctgggc cgacaggccc agcaccgcgt ccatcggcag gcgatcccgc aacacatgga
     4801 gcacggcccg tgtggccgcg taggcctgtt gccgcgagct ggccggcagc atggcctgca
     4861 cccgggccag ccagcggttg gtttcctgga cggtggtgtc gaacaccgga aggccggttg
     4921 tcatggcgcg cactccctga acgtcgcggg cccgcaaggc aggcggcgtt gcacagacgg
     4981 aaggatggcc cggcgccgga ccggctccgg acggcctgac ggccgcgcga agcccgttcc
     5041 gtctatcagg tggccgcgca accgcgttga ccgggatcaa accgccgcgc gacacgccgt
     5101 cgcggcgcgt gggcgacctc agccggtcgc cgaatcagcc tgggtcgccg ccagccggtc
     5161 gccggctcgc agcggcaggg ccacgcacgc ctggcgcagc acgaccgttc tctgacccct
     5221 ccgcacgaga tagccggtgt cttcgagctg cttgagcgcg cggctcaggg tctcgggctt
     5281 gaggctgaga aagtcggcga tgtcgcgata gttcatcggc aactcgatca ccccgggggc
     5341 gcgcgcgtcg tcggcatgcc gcagcagcca gttgaagaac cagatcagcc gctcgtgggc
     5401 cggcaggtga gtgatcttct cgacgtgatg ggccaggacg ccaaacaggg cgtttgatcg
     5461 gttgatcaac tcgaccacga cgtcaggtcg cgcgacgctc ttctccagca ccatgcgggt
     5521 catgaaccgg gtgacctcga cctccgacac ggcctcggcc gaggtgctgc gtcggtcgag
     5581 acaaaagccg aacaactcgc cgggaaacag gaaggcgacg atctgccgat cgccatcctc
     5641 accgttcacc tgcagccgca cgcagccggc ctcgagccga tacatgacgc cgggtggttc
     5701 gccctcgtca tagatgacct ggccgcgcgc catgtggaga cgtcggccag gcaccccttc
     5761 gagcagggaa tcgagatgcg tcaagaatcc ccccagggcc acggcgccaa cacttcggca
     5821 ttggcaacgt cggcgcggcg cctgccttga aatagatcac ccgttgaccg cgcgagggcc
     5881 gcgccggcgc cccgaaacgg gggtcgtggc gtcgcagggc gtccgggttc atgcgccagg
     5941 gggacagatg tccggcgcgc cctccggcgg ccatcgggcc gccttgaccc tcgtcaaggc
     6001 gcgcggggcg ccgtcgaccg agggtgtcgg ctcgacagcc gtgagctccg cgcatggacc
     6061 agcagcagac ggtttccccg gtctcgatcg gaccctggcg tctggccggc gacctgannn
     6121 nnnnnnnnnn nnnngtgatc ttcgcccatg ggtcgggctc cagccggctg agcccgcgca
     6181 atcaggaggt cgccgggtcc ctgtgcgacg cgggcctggc cacgctgctg tttgacctcc
     6241 tgaccgagga ggaggccgac gaccggtcca atgtgttcga tatcgatctg ctggccggcc
     6301 gtctggtcag cgcgatcgac tgggcccggg cgcggcccga cctggccgcc cttccggtcg
     6361 gcctgtttgg cgccagcacc ggcgcggcag cg
//
LOCUS       DAORNX010000130         6367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9040_length_6367_cov_6.810837, whole genome shotgun sequence.
ACCESSION   DAORNX010000130 DAORNX010000000
VERSION     DAORNX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9040_length_6367_cov_6.810837"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLO65_07210"
     CDS             <1..325
                     /locus_tag="PLO65_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HRD28071.1"
                     /translation="LTARTRPSSWPAALPDLRSRLNALTVAEIGPPDDALLIAVLEAL
                     LREKHIRAAPDLYEFLLRRMERSVAAARAVVARLDEAAAEQGRPVNRALAREVLEATG
                     DLFDA"
     gene            complement(396..1028)
                     /locus_tag="PLO65_07215"
     CDS             complement(396..1028)
                     /locus_tag="PLO65_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28072.1"
                     /translation="MGSTSDALELTPAGGRRRPKGDKRARTRARLLEAARDLVREKGY
                     EQVTLQDVARRAGMTSGAIYGNFRNRADLFVTLGETYWAPVRPLFHPDMTFAERMQAL
                     AEATIAAIPDRQEAVVGRLTGMAHALTDEELRRRVLAVTTASFGAGSAWLREAAQGVD
                     LPMPPDQLVVVIHAMTEGLLFQRFLTPELVSDDVFRAAFAALAGTPPLPA"
     gene            1099..2316
                     /locus_tag="PLO65_07220"
     CDS             1099..2316
                     /locus_tag="PLO65_07220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD28073.1"
                     /translation="MTTQQAITTALQAYVDKGQLAGAATLVWRDGRGAQTSCVGWRDR
                     ETGAPLMRDTIFRIASMSKPVTSVAALMLVDEGRIALDEPIANWAPDFTDMRVLRSAD
                     GPLDETDPAARPITFEDLLTHRSGLTYGGVHAGPITPAYAAALGGDIDSHVAPDDWIA
                     GLAALPLIDQPGASFHYSCSTDLLGLLVARIDGAPLGDVLRRRIFEPLGMKDTGFLVP
                     RDQRHRRATMYGFDEAGRLEPRLTAAGQPFLPERPDDMRFESGGQGLWSTLDDYLAFA
                     RLFVGGGAVDGVRLLRPETVARMTSNHLTDDQRARAEMLGMPVFTAHGFGLGVAVVLD
                     PDNAAAVRCKGGLGTVGWPGAYGGWWQADPTDSSVMVFLAHNLLELDRLAMGVGLDVY
                     SAITEVHALGSIV"
     gene            2409..3473
                     /locus_tag="PLO65_07225"
     CDS             2409..3473
                     /locus_tag="PLO65_07225"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRD28074.1"
                     /translation="MPNALGYAATAPDSGMAPFRFDRRALRPDDVKIAILYCGVCHSD
                     LHQARNDWGGTMYPCCPGHEIVGRVTAVGPQVRKFREGDLAAVGCLVDSCMDCDQCRK
                     GEEQLCRNRSTGTYNGKDRITGETTYGGYSDHIVVREAFVLKVPDSLDVTRVAPLLCA
                     GITTYSPLKTWKAGPGTRLGVAGLGGLGHMAVKFGVAMGCEVTVITGSESKRADALAL
                     GAHKVLLSGDREAMRDARNGYDLIIDTIPVAHRLQPYLPLLDIDGSLVIVGAIDELPS
                     FHSGLLLGGRRRVSGSPIGGIAETQEMLDFCAAHGVLPDCEMIAMQDINHAYERMERS
                     DVKYRFVIDMASLGKEKAAA"
     gene            3900..6104
                     /locus_tag="PLO65_07230"
     CDS             3900..6104
                     /locus_tag="PLO65_07230"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRD28075.1"
                     /translation="MTDAAITEVDPPAQGPRRPPLDEALLASPDRFFNRELSWLAFNR
                     RVLEEAENPRHPLLERLRFLSISANNLDEFYMVRVAGLKGQVREGVRVVSQDGLSPSE
                     QLVKVNASAAELMADQQRIWRELRTALQAEGIRLLEARDIGGADRDLAEHIFLNHVFP
                     VLTPLAIDPAHPFPFIPNLGFSLALKLRRPDGKELYALVPVPTAVRRFWPLPSEHGRS
                     RKGEQRFVALESLLVLFIDHLFPGCVVEGRGLFRLIRDSDVEIEEEAEDLVREFEAQL
                     KRRRLGEVVRVKVQTAMPPDLRAFILDSLHCDPEDVVLIDGKLGLAQMSQLIPEGRPD
                     LKFRPFVPRFPERIRDHDGDCFAAIREKDILVHHPFESFDVVVQFLRQAARDPHVLAI
                     KQTLYRTSQDSPIVAALIEAAENGKNVTALVEIKARFDEENNLRWAKDLERAGVHVVF
                     GFVEYKTHAKLSLVVRREGETLRTYCHFGTGNYHPVTAKIYTDLSLFTTDPALGRDSA
                     RLFNFITGYAPPQRLEKLSYSPETLKTDLMNMIAAEAENALAGKPSGIWVKLNALVDP
                     EIIDALYRASQAGVRIELVVRGICCLRPGVPGLSDNIRVKSIVGRYLEHSRIVAFANG
                     KTMPSNRARVFISSADWMPRNLDRRVEALTPVDNPTVHQQVLQQIMVANLNDEAQSWT
                     LDSQGGYHRDPAWDRPGAFSAHEYFMTNPSLSGRGHKVRDLPQAFDHVGPRR"
     gene            6085..>6367
                     /locus_tag="PLO65_07235"
     CDS             6085..>6367
                     /locus_tag="PLO65_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28076.1"
                     /translation="MLARVASEASLDGRARAARDAAVIDVGSNSVRLVLYRLEGRAIW
                     TVYNEKVLAGLGRDLGATGRLSPGGVEAALSALRRFRAVLDATAPGDVFT"
BASE COUNT          963 a         2282 c         2150 g          972 t
ORIGIN      
        1 gctcaccgcc cgcacccgtc cgtcgagctg gcccgccgcc ctgcccgacc tgcgctcgcg
       61 gctgaacgcc ctgaccgtgg ccgagatcgg cccgccggac gacgccctgc tgatcgccgt
      121 gcttgaagcc ctgctgcgcg aaaagcatat ccgcgccgcc ccggacctct acgaattcct
      181 gctgcgccgg atggaacggt cggtcgccgc cgcccgcgcc gtggtcgccc ggctcgatga
      241 ggccgccgcc gaacagggcc gtcccgtcaa ccgcgccctc gcccgcgagg tgctggaggc
      301 gaccggggat ctgttcgacg cgtagccttg atcgcgcttc gcaccttgca gcgagcgacg
      361 gtccgacggg gcctgctcgg gggaaggcgc accgctcatg ccggcaaggg cggggtcccc
      421 gccagcgccg cgaacgccgc gcggaatacg tcgtcgctca ccagttccgg ggtcaggaag
      481 cgctggaaca gcagcccctc ggtcatggcg tggatcacca ccaccagctg gtcgggcggc
      541 atcggcaggt cgacgccctg cgccgcctcg cgcagccagg cggagcccgc gccgaagctc
      601 gccgtcgtca ccgccagcac ccgccgtcgc aactcttcgt cggtcagggc gtgggccatg
      661 ccggtcagtc ggcccaccac ggcctcctgg cggtccggga tcgccgcgat ggtcgcctcg
      721 gccagggcct gcattctttc ggcgaaggtc atgtccgggt gaaacagcgg cctgaccggc
      781 gcccagtagg tctcgcccag ggtgacgaac aggtcggcgc ggttgcggaa gttgccgtag
      841 atcgcgccgc tggtcatgcc cgcgcgccgg gccacgtcct gcagggtgac ctgctcgtag
      901 cccttctcgc gcaccaggtc ccgcgccgct tccagcagcc gcgcccgcgt acgcgcccgc
      961 ttgtcgccct tcggtcgccg ccgcccgccc gcgggcgtca gctcaagcgc atctgacgtg
     1021 gagcccattg cctaccgtag tcaggttatt ttataatttg actatatttt tttccggatt
     1081 ggcggacaag aggcggcgat gaccacccag caggcgatca ccacggccct tcaggcctat
     1141 gtcgacaagg gacagctggc cggcgccgcg acgcttgtct ggcgcgacgg caggggggcg
     1201 cagaccagct gcgtcggctg gcgggaccgg gaaacgggcg ctccgctgat gcgcgacacg
     1261 atctttcgca tcgcctcgat gtccaagccc gtgacctcgg tcgccgccct gatgcttgtc
     1321 gacgagggcc gtatcgcgct ggacgagccg atcgccaact gggccccgga tttcaccgac
     1381 atgcgcgtgc tgaggtcagc cgacggtccg ctcgacgaga ccgatccggc ggcgcggccg
     1441 atcaccttcg aggacctgct gactcaccgc tcgggcctga cctacggcgg ggttcacgcc
     1501 ggcccgatca ccccggccta cgcggcggcg ctgggcggcg acatcgacag tcacgtcgcg
     1561 cccgacgact ggatcgcggg cctggccgcc ctgcccctga tcgaccagcc gggcgcgtcg
     1621 ttccactaca gctgctccac cgacctgctc ggcctgctcg tcgcccggat cgacggcgcg
     1681 ccgctggggg acgtgctgcg ccgacgcatc ttcgagccgc tgggcatgaa ggacacggga
     1741 ttcctcgtgc cccgcgacca gcgacatcga cgggcgacga tgtacggctt cgacgaggcc
     1801 gggcggctgg agccgcgact gaccgcggcc ggccagcctt tcctgccgga gcgaccggac
     1861 gacatgcgct ttgaatcggg cggtcagggc ctgtggtcga cgctcgacga ctacctggcc
     1921 ttcgcccgac tgttcgtcgg cggtggcgcg gtcgacggcg tgcggctgct gcggcccgag
     1981 accgtcgcgc ggatgaccag caatcatctg accgatgacc agcgggcgcg ggccgagatg
     2041 ctcggcatgc cggtgttcac ggcccatggc ttcgggctgg gcgtggcggt ggtgctggac
     2101 ccggacaacg ccgcggcggt ccgctgcaag ggcggcctgg gcaccgtcgg ctggccgggc
     2161 gcctatggcg gctggtggca ggccgatccg acggacagct ctgtcatggt cttcctggcc
     2221 cacaacctgc tcgaactgga tcgtctggcc atgggcgtag gcctcgacgt ctatagcgcg
     2281 atcaccgagg tccatgcgct ggggtcaatc gtctagggcc gtgcgccgtg gcgggggcgg
     2341 cttttggtgt cccgagggac aggataggct gtcgcccgag acgccccgcc ccctccggaa
     2401 ccgctcccat gcccaacgcc ctcggttacg ccgccaccgc ccccgacagc ggcatggcgc
     2461 cgtttcgctt cgaccgccgg gccctgcgac ccgacgacgt gaagatcgcc atcctctact
     2521 gcggcgtctg ccactccgac ctgcaccagg cgcgcaacga ctggggcggc acgatgtacc
     2581 cctgctgccc gggccacgag atcgttggcc gggtgacggc cgtcgggccg caggtgcgca
     2641 agttccgcga aggcgacctg gccgccgtcg gctgcctcgt cgacagctgc atggactgcg
     2701 accagtgccg caagggcgag gagcagctgt gccgcaaccg ctcgaccggc acctacaacg
     2761 gcaaggaccg gatcaccggc gagacgacct acggcggcta cagcgaccac atcgtggtgc
     2821 gcgaggcctt cgtgctgaag gtccccgaca gcctcgacgt gacccgcgtg gcgccgctgc
     2881 tgtgcgccgg gatcacgaca tactcgccgc tgaagacctg gaaggccgga cccggcacgc
     2941 ggctcggcgt agcgggcctg ggcggcctgg gccacatggc ggtcaagttc ggcgtggcca
     3001 tgggctgcga ggtgacggtg atcaccggct cggagtccaa gcgggcggac gccttggcgc
     3061 tgggcgcgca caaggtgctg ctgtccggcg accgggaggc catgcgcgac gcgcgcaacg
     3121 gctacgacct gatcatcgac accatcccgg tggcccacag gctgcagccc tatctgccgc
     3181 tgctcgacat cgacggcagc ctggtgatcg tcggggcgat cgacgagctg ccgtcgttcc
     3241 actccgggct gctgctgggc ggccgccgcc gtgtgagcgg ctcgcccatc ggcggcatcg
     3301 ccgagacgca ggagatgctc gacttctgcg ccgcgcacgg cgtgctcccg gactgcgaga
     3361 tgatcgccat gcaggacatc aaccacgcct acgagcggat ggagcggtcg gacgtgaagt
     3421 accgcttcgt catcgacatg gcgtcgctgg ggaaggagaa ggcggcggcg tagccgagca
     3481 taaagaggtg actctgctca cacgcagctc gaccacggaa gcagcgctcg attcgcttgg
     3541 cggcgcactc cgcccgatgt cttgacggcc agtgttgctc cggtggtgac cttgttcgag
     3601 gaccgccgtc ggcgcccgcg tagcggcgac cccgccgggg tggtccttga ccgaggtcat
     3661 cgaccggagg gcatcattcc atcaaggcca agggggagat gtcatcggtt tgatgaggat
     3721 atgcgctcca cctgccgcca ccggcgcctc cctctccacc atgctccgca tggcccccct
     3781 ctccctcgcg ggcgaggatt ggggtcaccc aaccgtaacg ccctccgtgt aacacccctg
     3841 tcatgccctc ttgcgacacg ccccgcgcca agcgacaacc cgctcgacgc agaggcccca
     3901 tgaccgacgc cgccatcaca gaggttgatc cgcccgccca gggaccccgc cggccgcctc
     3961 tcgacgaggc cctgctggcc tcgccggacc ggttcttcaa ccgcgaactg agctggctgg
     4021 cgttcaaccg gcgtgtgctg gaagaggcag aaaacccgcg ccatccgctg cttgagcggc
     4081 tgcggttcct gtcgatcagc gccaacaatc tcgatgaatt ctacatggtc cgcgtggccg
     4141 gcctgaaggg ccaggtgcgg gagggcgtgc gcgtggtcag ccaggacggg ctgagtccgt
     4201 ctgaacagct ggtcaaggtc aacgcctcgg cggccgagct gatggccgac cagcagcgca
     4261 tctggcgcga gctgcgcacc gccctgcagg ccgagggcat ccggctgctc gaggcccgcg
     4321 atatcggcgg ggccgaccgc gaccttgccg agcacatctt cctcaaccac gtgtttccgg
     4381 tgctcacgcc gctggccatc gacccggccc acccgttccc gttcattccc aacctgggct
     4441 tctcgctggc gctgaagctg cgccggccgg acggcaagga gctctacgcc ctggtgcccg
     4501 tgcccacggc cgtgcggcgg ttctggccgc tgccatccga gcatggccgg tcgcgcaagg
     4561 gcgagcagcg gttcgttgcg ctggaaagcc tgctggtcct gtttatcgac cacctgttcc
     4621 ccggctgcgt cgtcgagggc cgcggcctgt tccgcctgat ccgcgacagc gacgtcgaga
     4681 tcgaggaaga ggccgaggat ctggtccgcg agttcgaggc gcagctgaag cgacggcggc
     4741 tcggcgaggt ggtgcgggtc aaggtgcaga cggccatgcc gccggacctg cgcgccttca
     4801 ttctcgacag cctgcactgc gaccccgagg acgtggtgct gatcgacggc aagctcggcc
     4861 tggcccagat gtcgcagctg atccccgagg ggcggcccga tctgaaattc aggcccttcg
     4921 tgccgcgctt ccccgagcga atccgcgacc acgacggtga ctgtttcgcc gccatccggg
     4981 agaaggacat cctcgttcac cacccgttcg agagtttcga cgtggtggtc cagttcctgc
     5041 gccaggccgc ccgcgacccg catgtgctgg cgatcaagca gacgctctac cgcaccagcc
     5101 aggacagccc gatcgtcgcg gccctgatcg aggcggccga gaacggcaag aacgtcaccg
     5161 ccctggtcga gatcaaggcg cgcttcgacg aggagaacaa cctgcgctgg gccaaggacc
     5221 tggagcgggc gggcgtgcac gtggtgttcg gcttcgtcga gtacaagacc cacgccaagc
     5281 tgagcctggt ggtccgccgc gagggcgaga ccctgcgcac ctactgccac ttcggcaccg
     5341 gcaactacca cccggtgacg gccaagatct acaccgacct cagcctgttc acgaccgacc
     5401 cggcgctggg gcgcgactcg gcgcggctgt tcaacttcat caccggatac gccccgccgc
     5461 agcggctcga gaagctgagc tacagccccg agaccctgaa aaccgacctg atgaacatga
     5521 tcgcggccga ggccgagaac gccctggccg gcaagccctc gggcatctgg gtcaagctca
     5581 acgccctggt cgacccggag atcatcgatg ccctttatcg cgccagccag gcgggcgtgc
     5641 gcatcgaact ggtcgtgcgc ggcatctgct gcctgcggcc gggcgtgccg ggcctcagtg
     5701 acaacatcag ggtcaagagc atcgtcggcc gctatctcga gcacagccgt atcgtggcct
     5761 tcgccaacgg caagaccatg ccgagcaaca gggccagggt gttcatcagc tcggccgact
     5821 ggatgccgcg caatctcgac cggcgggtcg aggccctcac cccggtggac aacccgaccg
     5881 ttcaccagca ggtgctgcag cagatcatgg tcgccaatct caacgacgag gcgcagagct
     5941 ggacgctgga cagccagggc ggctatcacc gcgatcccgc ctgggaccgg ccgggcgcct
     6001 tctccgccca tgagtatttc atgaccaacc ccagcctgtc gggccgtggc cacaaggtgc
     6061 gcgacctgcc gcaagcgttc gatcatgttg gcccgcgtcg ctagcgaggc gagcctggac
     6121 ggccgggcgc gcgccgcgcg cgacgccgcc gtcatcgacg taggctccaa ttccgtgcgc
     6181 ctggtgctct accgcctcga gggccgggcc atctggacgg tctacaacga gaaggtgctc
     6241 gccggtctcg gccgcgatct gggggccacc ggccggctgt cgccgggcgg ggtcgaggcg
     6301 gccctgtcgg ccctgcgccg gttccgcgcc gtgctggacg ccaccgcccc gggcgacgtg
     6361 ttcaccg
//
LOCUS       DAORNX010000131         6317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9142_length_6317_cov_12.714309, whole genome shotgun sequence.
ACCESSION   DAORNX010000131 DAORNX010000000
VERSION     DAORNX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_6317_cov_12.714309"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /gene="rpsH"
                     /locus_tag="PLO65_07240"
     CDS             complement(<1..228)
                     /gene="rpsH"
                     /locus_tag="PLO65_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRD28077.1"
                     /translation="MVNDPIGDMIARIKNAAMRKRAKVSTPASKMRARVLDVLAGEGY
                     IRGYSLVEKPGAFPEFEIELKYFDNEPVIVEI"
     gene            complement(244..549)
                     /gene="rpsN"
                     /locus_tag="PLO65_07245"
     CDS             complement(244..549)
                     /gene="rpsN"
                     /locus_tag="PLO65_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRD28078.1"
                     /translation="MAKKSAVNRNEMVKKLVKQYAAKRAALKAVANDETLPLEERFDA
                     RLKLAKLPRNSAANRIRNRCEVTGRPRAYYRKLKMSRIALRELGSAGLIPGLVKSSW"
     gene            complement(571..1128)
                     /gene="rplE"
                     /locus_tag="PLO65_07250"
     CDS             complement(571..1128)
                     /gene="rplE"
                     /locus_tag="PLO65_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRD28079.1"
                     /translation="MADQAYEPRMKTIYRERIRAAMKEQFGYSNEMQVPKLDKIVLNM
                     GIGEAVADSKKAAAAMKDLAAIAGQKPVGTKARLSVAQFKIREGMTIGAKVTLRADRM
                     YEFLDRLITIALPRVKDFRGLKPTSFDGRGNYAMGLKEHIVFPEINYDQIDQIWGMDI
                     IVCTTAKTDDEARALLKEFQFPFTA"
     gene            complement(1121..1435)
                     /gene="rplX"
                     /locus_tag="PLO65_07255"
     CDS             complement(1121..1435)
                     /gene="rplX"
                     /locus_tag="PLO65_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRD28080.1"
                     /translation="MAAKIKKGDKVVLLTGKDKGKTGAVVRVFPKEERVVVGGLNMVQ
                     RHTKPTQADPQGGIKHKEAPVHVSNVAIVDSNGKPTRVGFRMDGDKKVRFAKTTGEAI
                     NG"
     gene            complement(1435..1803)
                     /gene="rplN"
                     /locus_tag="PLO65_07260"
     CDS             complement(1435..1803)
                     /gene="rplN"
                     /locus_tag="PLO65_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRD28081.1"
                     /translation="MIQMQTNLEVADNSGARRVMCIKVLGGAKRRYAHVGDKIVVSVK
                     EAIPRGRVKKGDVLQAIVVRTSSAIKRKDGSVIRFDKNAAVIVNKQAEPIGTRIFGPV
                     PRELRAKNHMKIISLAPEVL"
     gene            complement(1824..2063)
                     /gene="rpsQ"
                     /locus_tag="PLO65_07265"
     CDS             complement(1824..2063)
                     /gene="rpsQ"
                     /locus_tag="PLO65_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRD28082.1"
                     /translation="MPKRILEGVVVSDKGDKTVVVKVERTFLHPLLKKTVRRSKKYHA
                     HDEANAHKAGEIVKIVECAPKSKLKTWEVLPKAVS"
     gene            complement(2074..2265)
                     /gene="rpmC"
                     /locus_tag="PLO65_07270"
     CDS             complement(2074..2265)
                     /gene="rpmC"
                     /locus_tag="PLO65_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRD28083.1"
                     /translation="MKIDEVRGMTPDQLAEALLNLKKEQFNLRFQAATGQVEKTHRVN
                     EIRKDIARIKTVLRAKKAA"
     gene            complement(2268..2699)
                     /gene="rplP"
                     /locus_tag="PLO65_07275"
     CDS             complement(2268..2699)
                     /gene="rplP"
                     /locus_tag="PLO65_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRD28084.1"
                     /translation="MLSPKKTKYRKAFKGRIHGTAKGGTLLNFGSYGLKAVEPERITA
                     RQIEAARRAITRHMKRQGRVWIRIFPDLPVTDKPAEVRMGKGKGAVDYWAARVHPGRI
                     MFEIDGVADDIARDALKLGAAKLPIKTRIVARIDSGLAAEH"
     gene            complement(2705..3463)
                     /gene="rpsC"
                     /locus_tag="PLO65_07280"
     CDS             complement(2705..3463)
                     /gene="rpsC"
                     /locus_tag="PLO65_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRD28085.1"
                     /translation="MGQKVNPVGLRLGVNRTWDSRWFADGKEYGRLLHEDIKVRRELK
                     KRLYQAGISRIIIERPHKKCRVTIYAARPGVIIGKKGADIDKLRKDIASMVGVEVHLN
                     IVEIRKPETDAQLVAENIAQQLERRVAFRRAMKRTMQSTMRLGAKGVRINVSGRLGGA
                     EIARMEWYREGRVPLHTLRADIDYGFAEAKTTYGIIGVKTWIFKGEVLDHDPMALDKR
                     LATESGPAGEGGGRDRDDRGPRGPRRDRGRGSEA"
     gene            complement(3463..3843)
                     /gene="rplV"
                     /locus_tag="PLO65_07285"
     CDS             complement(3463..3843)
                     /gene="rplV"
                     /locus_tag="PLO65_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRD28086.1"
                     /translation="MSKKAKARRLPATEVQAKLRTLRTSARKLNLVAQSIRGLKVQRA
                     LNELEFSHKRIAKDVRKALYSAISNAENNHNLDIDSLVVSEAFVGKNLVMKRFSARAR
                     GRSSRIEKPFSELTIVVRELGEAA"
     gene            complement(3845..4123)
                     /gene="rpsS"
                     /locus_tag="PLO65_07290"
     CDS             complement(3845..4123)
                     /gene="rpsS"
                     /locus_tag="PLO65_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRD28087.1"
                     /translation="MTRSVWKGPFVDGYLLKKAETAQASGRKDVIKTWSRRSTIMPQF
                     VGLVFGVHNGLKHVPVSVSEDMVGHKLGEFAPTRNFPGHAADKKAKRK"
     gene            complement(4127..4966)
                     /gene="rplB"
                     /locus_tag="PLO65_07295"
     CDS             complement(4127..4966)
                     /gene="rplB"
                     /locus_tag="PLO65_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRD28088.1"
                     /translation="MALKQYNPTSPGRRSLVLIDRSELHKGRPEKSLVEGLTKSGGRG
                     GGGRVAVRFRGGGAKRLYRMVDFKRRKWNIAATVERLEYDPNRTAFIALIKYADGELA
                     YILAPQRLKVGDEVIASEKVDVKPGNAAPLSGLPIGTIIHNVELKPMKGGQLARSAGA
                     YAQLVGRDGGYAQVRLGSGELRMVQDTCMATVGAVSNQDHMNQVLGKAGRVRHMGFRP
                     HVRGVAMNPVDHPHGGGEGRTSGGRHPVTPAGKPTKGAKTRTNKATDKFIIRSRHKAK
                     KGR"
     gene            complement(4970..5266)
                     /locus_tag="PLO65_07300"
     CDS             complement(4970..5266)
                     /locus_tag="PLO65_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRD28089.1"
                     /translation="MSATARHYDTILAPVITEKATYLSEQNKVVFRVADDATKDEIAA
                     AVEALFKVKVTKVNTLNVKGKTKRFRGRVGHRSDVKKAIVTLADGQSIDITTGL"
     gene            complement(5266..5904)
                     /gene="rplD"
                     /locus_tag="PLO65_07305"
     CDS             complement(5266..5904)
                     /gene="rplD"
                     /locus_tag="PLO65_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRD28090.1"
                     /translation="MKLDVIKLDGAKAGSVDLDDAIFGIDEIRGDILQRVVTWQLAKR
                     RSGNHKIQVRNEVSRTGKKMYKQKGTGGARHGSRRAAQFVGGAKAHGPVVRSHEFGLN
                     KKIRALALRHALSSKAKSGSLVVIDAAELADPKTAGLRATFDKMGLKNALIIAGPEVN
                     ANFKLAARNIPNIDVLPNAGLNVYDVLRRGTLVLTKSAVDGISARFAQKEAA"
     gene            complement(5904..>6317)
                     /gene="rplC"
                     /locus_tag="PLO65_07310"
     CDS             complement(5904..>6317)
                     /gene="rplC"
                     /locus_tag="PLO65_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRD28091.1"
                     /translation="KGFAGAMKRWNFGGMRATHGVSVSHRAHGSTGQRQDPGKTFAGK
                     KMAGHLGQETVTTMNLTVWKVDVERGLILIKGAVPGTEGSYVKVRDAIKKAAPADLPR
                     PGAFRKSGEAAAAAPAEEPQVEEAAAPEATQEGAE"
BASE COUNT          899 a         2014 c         2117 g         1287 t
ORIGIN      
        1 gatctcgacg atcacgggct cgttgtcgaa gtacttgagc tcgatctcga attcggggaa
       61 cgcgccgggc ttctcgacca gcgagtagcc gcggatgtag ccctcgccgg ccagcacgtc
      121 gaggacgcgc gcgcgcatct tcgaggccgg ggtggagact ttcgcccgct tgcgcatggc
      181 ggcgttcttg atgcgggcga tcatgtcgcc aatggggtcg ttgaccatct tacgatccct
      241 cccttaccag ctcgacttga cgaggccggg gatcagaccg gcggagccaa gctcacgcag
      301 ggcgatccgg ctcatcttca gcttgcgata gtaggcgcgc ggacgacccg tcacctcgca
      361 ccggttacgg atgcggttgg cggcggaatt ccgcggcagc ttcgccagct tcagccgggc
      421 atcgaaacgt tcttcgagcg gcagggtctc gtcattggcg acagccttca gcgcggcgcg
      481 cttggcggcg tactgcttga cgagcttctt gaccatctcg ttacggttga cggcgctttt
      541 cttggccatg agttctttcc ttcccgtccg ttacgcggtg aacgggaact ggaattcctt
      601 gagaagcgcg cgcgcttcgt cgtcggtctt cgcagtggtg cagacgatga tgtccatgcc
      661 ccagatctgg tcgatctggt cgtagttgat ctccgggaac acgatgtgtt ccttcaggcc
      721 catggcgtag ttgccacggc cgtcgaagga ggtcggcttg agaccccgga agtccttcac
      781 ccgcggcagg gcgatggtga tcaggcgatc gagaaactcg tacatgcggt cggcgcgcag
      841 ggtgaccttg gcgccgatgg tcatgccttc ccggatcttg aactgggcga cggacaggcg
      901 ggccttggtt ccgaccggct tctggccggc gatcgccgcc aggtccttca tcgccgcggc
      961 ggccttcttg gagtcggcca cggcctcgcc gatgcccatg ttcaggacaa tcttgtccag
     1021 cttgggaacc tgcatctcgt tggaatagcc gaactgttcc ttcatcgcgg cgcggatgcg
     1081 ctcgcgatag atggtcttca tccggggttc gtaagcctga tcagccattg atcgcctcgc
     1141 ccgtggtctt ggcgaaccgc accttcttgt cgccgtccat gcggaagccg acgcgagtgg
     1201 gtttgccgtt ggagtcgacg atggccacgt tcgacacgtg caccggcgct tccttgtgtt
     1261 tgatgccgcc ctgcgggtcc gcctgggtcg gcttggtgtg gcgctggacc atgttgagcc
     1321 cgccgacgac gacgcgctct tccttcggga agacgcgaac gacggcgccg gtcttgccct
     1381 tgtccttgcc ggtgaggagg acgaccttgt cgcccttctt gatcttggcc gccatcacag
     1441 cacctccggc gcgagggaga tgatcttcat gtggttcttg gcgcgcagtt cgcggggaac
     1501 cgggccgaag atccgcgtgc cgatcggctc cgcctgcttg ttgacgatca ccgccgcgtt
     1561 cttgtcgaag cggatgaccg agccgtcctt gcgcttgatg gccgaggacg tgcgaacgac
     1621 gatggcttgc agcacatcgc ccttcttcac gcggccgcgc ggaatggctt ccttgacgga
     1681 gacaacgatc ttgtccccga cgtgtgcgta gcggcgtttg gcgccgccaa gcaccttgat
     1741 gcacatgacc cggcgcgcgc ccgaattgtc ggcgacttcc aggttagtct gcatctggat
     1801 catggtttaa taccttccag aaactagctg acggccttgg gaaggacttc ccaggtcttc
     1861 agtttggact tcggcgcgca ctcgacgatc ttgacgatct cgcccgcctt gtgggcgttg
     1921 gcttcgtcgt gagcgtgata cttcttcgac cgacggacgg tcttcttcag cagcgggtgc
     1981 aggaaggtcc gctcgacctt caccaccacc gtcttgtcgc ccttgtcgga gacgaccacg
     2041 ccttcaagaa tacgtttggg catatcggtc tccttacgcg gccttcttgg cgcgcagcac
     2101 ggtcttgatc cgcgcgatat ccttgcggat ctcgttcacg cggtgggtct tctcgacctg
     2161 gccggtcgcc gcctggaagc gcaggttgaa ctgctccttc ttcaggttca gcagggcctc
     2221 ggccagctgg tcgggggtca tcccccggac ttcatcgatc ttcatcttca gtgctccgcg
     2281 gccaggccgg aatcgatccg ggcgacgatg cgcgtcttga tcggcagctt ggcggcgccg
     2341 agcttgagcg cgtcacgggc gatgtcgtcg gcgacgccgt cgatctcgaa catgatgcga
     2401 cccgggtgga cacgagcggc ccagtaatcg acggcgccct tgcccttgcc catccgcact
     2461 tcggccggct tgtcggtgac cggcaggtcc gggaagatgc ggatccagac gcgaccctgg
     2521 cgcttcatgt ggcgggtgat cgcgcggcgg gccgcctcga tctgacgcgc cgtgatgcgc
     2581 tccggctcaa cggccttgag gccgtaggag ccgaagttca gcagggtgcc gcccttggcg
     2641 gtcccgtgga tccggccctt gaaggccttc cggtacttgg ttttcttcgg tgacagcatg
     2701 acggttacgc ctcagatccg cgaccgcggt cgcggcgcgg gccgcggggt ccgcgatcgt
     2761 cacggtcacg accgccgcct tcgccggcgg ggccggattc ggtggccaga cgcttgtcca
     2821 gcgccatcgg gtcgtggtcc agcacctcgc ccttgaagat ccaggtcttc acgccgatga
     2881 tgccgtaggt ggtcttggct tccgcgaagc cgtagtcgat gtccgcgcgc agggtgtgca
     2941 gcggcacgcg accttcgcga taccattcca tccgcgcgat ctcggcgccg cccaggcggc
     3001 ccgacacgtt gatccggacg cccttggccc ccagacgcat ggtcgactgc atggtgcgct
     3061 tcatggcgcg acggaaggcg acccggcgct cgagctgctg ggcgatgttc tcggccacca
     3121 gctgggcgtc ggtctcgggc ttgcggatct cgacgatgtt caggtggacc tcgacgccga
     3181 ccatggacgc gatgtccttg cgcagcttgt cgatgtcggc gcccttcttg ccgatgatca
     3241 cgccagggcg cgcggcatag atggtcacgc ggcacttctt gtgcggacgc tcgatgatga
     3301 tccgcgagat gccggcctgg tagaggcgct tcttcagttc gcgacggacc ttgatgtcct
     3361 cgtgcagaag gcgaccgtat tccttgccgt cggcgaacca gcggctgtcc caggtgcggt
     3421 tgacgccgag ccggagcccg accggattga ctttctgacc catcaggcgg cctcacccag
     3481 ttcgcgaacc acgatggtga gctcgctgaa cggtttctcg atacgcgacg agcggccccg
     3541 cgcgcgggcg gagaagcgct tcatcaccag gttcttgccc acgaaggcct cggacacgac
     3601 cagcgagtcg atgtcgaggt tgtggttgtt ctcggcgttc gagatggcgc tgtagagcgc
     3661 cttgcggacg tccttggcga tgcgcttgtg gctgaactcg agttcgttca gcgcgcgctg
     3721 caccttcagg ccgcggatcg actgggccac gaggttgagc ttccgggccg aggtgcgcag
     3781 cgtgcgcagc ttggcctgga cctcggtcgc gggcagtcga cgggctttcg ctttcttcga
     3841 catgctactt cctcttggcc ttcttgtcgg ccgcgtgacc cgggaagttc cgcgtcgggg
     3901 cgaattcgcc gagcttgtgg ccgaccatgt cttccgacac ggagacgggc acgtgcttga
     3961 gaccgttgtg gacgccgaac accaggccga cgaactgcgg catgatggtc gagcggcgcg
     4021 accaggtctt gatcacatcc ttgcggccgg aggcttgcgc ggtttctgcc ttcttcagca
     4081 ggtagccgtc gacaaacggg cccttccaga ctgagcgggt catggcttaa cggcccttct
     4141 tcgctttgtg gcgcgaccgg atgatgaact tgtccgtggc cttgttggtg cgggtcttgg
     4201 cgcccttggt cggcttgccg gccggggtca ccgggtggcg gccgcccgag gtgcggcctt
     4261 cgccgccgcc gtgcgggtgg tcgaccgggt tcatggcgac gccgcgaacg tgcggacgga
     4321 agcccatgtg gcggacgcgg ccggccttgc ccaggacctg gttcatatgg tcctggttgg
     4381 agacggcgcc gaccgtggcc atgcaggtgt cctggaccat gcgcaactcg cccgatccga
     4441 ggcggacctg ggcgtagccg ccgtcacggc caaccagctg ggcgtaggcc ccggcggagc
     4501 gcgccagctg gccgcccttc atgggcttga gctcgacgtt gtggatgatc gtgccgatcg
     4561 gcaggccgct cagcggggcg gcgttgcccg gcttcacgtc gaccttctcg gacgcgatca
     4621 cctcgtcgcc gaccttgagc cgctgcgggg ccaggatgta ggccagctcg ccgtcggcgt
     4681 acttgatcag ggcgatgaag gccgtccggt tcggatcgta ctccaggcgc tcgaccgtcg
     4741 cggcgatgtt ccacttgcga cgcttgaagt cgaccatgcg gtagaggcgc ttggcgccgc
     4801 cgccacggaa gcggaccgcg acacggccac cgccgccacg accgcccgac ttggtcaggc
     4861 cttcgacgag cgacttttcc ggacggccct tgtggagctc gctgcggtcg atcagcacca
     4921 ggctgcgacg gcccggcgat gtcgggttgt actgcttcag agccatctct tagagccccg
     4981 tcgtgatgtc gatcgactgg ccatcggcca gcgtcacgat ggctttcttg acgtcggacc
     5041 gatgtccgac gcggccgcgg aagcgcttgg tcttgccctt gacattgagg gtgttgacct
     5101 tggtcacctt gaccttgaac agcgcttcga ccgcggcggc gatctcgtcc ttcgtcgcgt
     5161 cgtcggccac gcggaagacg accttgttct gttcggacag ataggtcgcc ttctcggtga
     5221 tcaccggggc gaggatggtg tcgtaatggc gggcggtggc tgacattacg cggcttcctt
     5281 ctgggcgaag cgggccgaga tgccgtcgac ggccgacttg gtcaggacca gggtcccgcg
     5341 ccgcagcacg tcgtagacgt tcaggccggc gttcggcagc acatcgatgt tcggaatgtt
     5401 gcgagccgcc agcttgaagt tggcgttcac ttccggcccg gcgatgatca gggcgttctt
     5461 cagacccatc ttgtcgaagg tggcgcgcag accggccgtc ttcggatcgg ccagctcggc
     5521 ggcgtcgatg accaccagcg agcccgactt ggccttggac gacagcgcat ggcgcagggc
     5581 cagggcgcgg atcttcttgt tcaggccgaa ctcgtggctg cggacgaccg ggccgtgagc
     5641 cttggcgccg ccgacgaact gggccgcccg gcgcgagccg tgacgggcgc cgccggtgcc
     5701 cttctgcttg tacatcttct tgcccgtacg agagacctcg ttgcggacct ggatcttgtg
     5761 gttgccggag cggcgcttgg ccagctgcca ggtgacgacg cgctgaagga tgtccccgcg
     5821 gatttcgtcg atgccgaaga tggcgtcgtc gagatcgacc gaaccggcct tggcgccgtc
     5881 aagcttgatg acgtcgagtt tcattattca gcgccctctt gcgtggcttc gggggccgcg
     5941 gcctcttcaa cctggggctc ttcggccgga gcggcggcgg cggcctcgcc cgacttgcgg
     6001 aaggcgcccg gacgcggcag gtcggccgga gcggccttct tgatcgcgtc gcgcaccttc
     6061 acataggagc cttcggtgcc ggggacggcg cccttgatca ggatcaggcc gcgctcgacg
     6121 tccaccttcc agaccgtgag gttcatggtg gtgacggttt cctgacccag atggccggcc
     6181 atcttcttgc cggcgaaggt cttgcccgga tcctgacgct ggccggtcga accgtgagcc
     6241 cggtgcgaga ccgagacacc gtgcgtggcg cgcatgccgc cgaagttcca gcgcttcatg
     6301 gcgccggcga agccctt
//
LOCUS       DAORNX010000132         6285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9210_length_6285_cov_7.257785, whole genome shotgun sequence.
ACCESSION   DAORNX010000132 DAORNX010000000
VERSION     DAORNX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_6285_cov_7.257785"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /gene="rpsR"
                     /locus_tag="PLO65_07315"
     CDS             complement(<1..176)
                     /gene="rpsR"
                     /locus_tag="PLO65_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRD28092.1"
                     /translation="MSDTDISPEAPAAAPAAGARRPFYRRRKVCPFSGAGAPKIDYKD
                     VKLLQRYVSERGKIV"
     gene            complement(191..586)
                     /gene="rpsF"
                     /locus_tag="PLO65_07320"
     CDS             complement(191..586)
                     /gene="rpsF"
                     /locus_tag="PLO65_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRD28093.1"
                     /translation="MALYEHVLIARQDISPQQAEQLNEDMKALIESLGGHVAKIEYWG
                     LRNLTYRIKKNRKGHYSLMAIDAPAPAVKEMERQLSINEDVLRYMTIRVEELDLELSP
                     VLARRDRDRSDRPERSERGDRPREDFAAA"
     gene            819..1352
                     /locus_tag="PLO65_07325"
     CDS             819..1352
                     /locus_tag="PLO65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HRD28094.1"
                     /translation="MNRTISACFCAVAILLPAGSPAAAAEGAAAPSRRALDCARRYSD
                     AMNMEETMAGMMTGMLPSILEQERRRSGASFTARQERLMGEAMTESIKAMVPEMMEQL
                     TPLMAVAFTEDELCALADFYASKEGQSVIAKMPAFTASSMAAMQDFIPRFERDMEMRF
                     CRKISCDPAKIPTPQPS"
     gene            complement(1356..1898)
                     /locus_tag="PLO65_07330"
     CDS             complement(1356..1898)
                     /locus_tag="PLO65_07330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD28095.1"
                     /translation="MKRLAAAALALSLVAAPAYAALKVGAVAPDFSTTAFKGGQASPF
                     RLSSTLKTGKVVVLYFFPSAYTSGCTAEAHEFAVATPEFAAMGASVIGVTAGRVEDLA
                     KFSREECRDAFPVAGVTKETIKAYDAVLAFKPDLSDRTTYVIAPDGKILLAYTALTDP
                     LSHIAKAKEAVAAWRRAHPK"
     gene            complement(1956..2921)
                     /locus_tag="PLO65_07335"
     CDS             complement(1956..2921)
                     /locus_tag="PLO65_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28096.1"
                     /translation="MKRFVCALIVALTFGGPAFAAPRPPESVRQAVQDRIDAAMAPDP
                     GDVDDSGEPYDPPDALAGPKMFKRVEVNNDGVADWRVNFEQAPNPSYFCGTGGCRQQI
                     FVSNPRGGFDLAMDTQVRLFKLRHKRGQTLLDVDFHGSVCGGFGVDDCPRGYVWSVAE
                     ARFVARVDPGAQAFLIGGPVWLTTPSRASLPSAVEAAFAERAARCAAAGRDYPYDEAY
                     LTEVGDLNADGRADWVVGGAYDGCAYREEAAAAPRFDLTVLVSGPDGYHSALNSDVTT
                     WGLDMADGKARFVTLEGDDCGLNGKDCPKTYWRWDGQALVRQPLP"
     gene            complement(3029..4102)
                     /locus_tag="PLO65_07340"
     CDS             complement(3029..4102)
                     /locus_tag="PLO65_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD28097.1"
                     /translation="MRAVVRRAGKLVCDTIEERDPGPGQVRVKTLACGICGSDLHALH
                     YLDHMVEMGRRAGAGGGMDPKQDIVFGHEFCAEVLEGGPGANRFKAGTRVVSMPVNMG
                     PEGFDAVGYSNKYPGGFAERMILTEALLLEVPNGLSTDHAALTEPMAVGAHAVASAAL
                     DSNSVSLVLGCGPVGLAVIAALKVRGHGPVIAADFSPRRRAAAEAMGADVVVDPAETG
                     PHTRWAEFGVPSTLDGMRMALFGGQTYGRPVVFECVGSPGVLQSVIEGCPPKAQIIVA
                     GVCMEPDRIEPSLAINKQLDLRFVLGYQPDEFAQTLSDIAEGRINVDPLITARVGLAG
                     VAQAFVDLGNPESNVKILVDPGL"
     gene            complement(4352..4981)
                     /locus_tag="PLO65_07345"
     CDS             complement(4352..4981)
                     /locus_tag="PLO65_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRD28098.1"
                     /translation="MPAYRLAIFDFDGTLGDTLGWMLDTSDALAEKFGYRKIDRSQLD
                     QLRHLSAREMMKVQKLPAVKLPAIAAHFQKLMTADAGRIAMFDGVPEMLRALHGAGVR
                     LAIVSSNSEENVRIILGPDLCGLVSMFNCGASMFGKAPKFRKVLKRLEVRPGEAISVG
                     DEIRDLDAAREVGLATGVVCWGYTAPDALKAQKPDHVFETVEAIAARLS"
     gene            5088..6032
                     /gene="fabD"
                     /locus_tag="PLO65_07350"
     CDS             5088..6032
                     /gene="fabD"
                     /locus_tag="PLO65_07350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRD28099.1"
                     /translation="MSLAFIFPGQGSQTVGMGAELAEAFASAREVFQEVDDALSQKLS
                     TLMREGPEADLTLTENAQPALMAVSMAVMRTLEREMGVPVEKAAFVAGHSLGEYAALA
                     AAGAISLADTARLLKLRGQAMQRAVPVGQGAMASLIGPKTDVALAEAAAAAGAEVGVC
                     VVANDNNNGNVVISGDRAGVDRAIEKAKELGARAIPLNVSAPFHCPLMQPAADEMAAA
                     LAGTTILAPRAPLVANVTARPTLDPEAIRRMLVEQVTGRVRWRESMEWLAGEGGVTRF
                     AEAGAGKVLSGMAKRIAPDAEATPLNTPADLEAFAKSL"
     gene            6154..>6285
                     /locus_tag="PLO65_07355"
     CDS             6154..>6285
                     /locus_tag="PLO65_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28100.1"
                     /translation="MFDLTGKTALVTGATGGIGGAIARALHAQGAHVVLSDTRETALE
                     "
BASE COUNT          986 a         2092 c         2235 g          972 t
ORIGIN      
        1 acgatcttgc cgcgctcgga aacgtagcgc tgcaggagct tgacgtcctt gtagtcgatc
       61 ttcggcgcgc cggcgcccga gaacgggcag accttgcggc ggcgatagaa cgggcggcgg
      121 gcgccggcgg ccggggcggc ggccggagct tcgggggaga tatcggtatc ggacatgatc
      181 agtttctccc tcaggccgcg gcgaagtctt cacgcgggcg gtcgccgcgc tcggagcgtt
      241 cggggcgatc gctgcgatcg cggtcgcggc gggcgagaac cggcgacagt tccaggtcga
      301 gttcctcgac gcggatggtc atgtagcgca gcacgtcttc gttgatcgac agctggcgct
      361 ccatttcctt gacggccggc gccggggcgt cgatggccat cagggaatag tgacccttgc
      421 ggttcttctt gatgcggtag gtgaggttgc gcaggcccca gtactcgatc ttggcgacgt
      481 ggccgccaag gctttcgatc agggccttca tgtcctcgtt gagttgctcg gcctgttggg
      541 gcgagatgtc ttgccgtgcg atcagcacgt gctcgtaaag agccatcagg ttcctcttcc
      601 gttgtggagg gcggcgcggc cggggaggga agtgtccccg gtcacgatgg ctctgtcgcg
      661 gcggccgggc gtttcccgac ctgaaaggct ccgcgagaga ccgccttccg tgaagggcgc
      721 gctagtaagc gagattggcg gtaaaggcaa gcgcgccgag ggattgaagt ccgtcctggg
      781 gttgtgccag cctgtcgccc tcaatcgggg ggctttcaat gaaccggacg atcagcgcct
      841 gcttctgcgc cgtcgcgatc ctgttgcctg cgggatcgcc cgctgcggcg gccgagggcg
      901 cggcggctcc ctcgaggcgg gcgcttgact gcgcccgtcg ctacagcgac gccatgaaca
      961 tggaagagac gatggccggg atgatgaccg gcatgctgcc gtcgatcctg gagcaggaac
     1021 gccgtcgaag cggcgccagc ttcaccgcca ggcaagagcg gctgatgggt gaggcgatga
     1081 ccgagagcat caaggcgatg gttccggaaa tgatggaaca gctgacgccg ttgatggcgg
     1141 tggcgttcac cgaggatgaa ctgtgcgcgc tggcggactt ctacgccagc aaggagggcc
     1201 agagcgtcat cgccaagatg ccggccttca ccgccagttc gatggcggcc atgcaggact
     1261 tcatcccgcg gttcgagcgc gacatggaga tgcggttctg ccgcaagatc agctgcgacc
     1321 cggcgaagat accgactccg cagccttcct gatccctact tcgggtgcgc cctgcgccag
     1381 gcggcgaccg cttccttggc cttggcgatg tgcgacagcg ggtcggtcag ggccgtgtag
     1441 gccagcagga tcttgccgtc gggcgcgatc acataggtgg tgcggtccga caggtccggc
     1501 ttgaacgcca ggacggcgtc gtaggccttg atggtctcct tggtcacccc ggccaccggg
     1561 aaggcgtcgc ggcattcttc cctggagaac ttggccaggt cctcgacccg accggcggtg
     1621 acgccgatca cgctggcgcc catggcggcg aattccggcg tggccacggc gaactcgtga
     1681 gcctccgccg tgcagcccga ggtgtaggcc gacgggaaga agtacagcac cacgaccttg
     1741 ccggtcttga gggtgctcga cagcctgaag ggcgaggcct gtccgccctt gaaggccgtg
     1801 gtgctgaagt cgggggccac ggcgccaacc ttcagcgcgg cataggccgg ggcggcgacg
     1861 agggacaggg cgagggcggc ggcggcaagg cgtttcatgg cggatctccc ggttgccgga
     1921 atagttaggc cgtccggcgt ccgcgtccag tgaacttagg ggagcggttg cctgaccagg
     1981 gcctgaccgt cccaccgcca gtaggttttc gggcagtcct tgccgttcag gccgcagtcg
     2041 tcgccctcca gcgtcacgaa gcgggccttg ccgtcggcca tgtccaggcc ccaggtggtg
     2101 acgtcgctgt tcagggcgct gtggtagccg tccgggccgc tgaccagcac agtcaggtcg
     2161 aagcgcggcg ccgccgcggc ttcttcccgg taggcgcagc cgtcgtatgc gccgccgacg
     2221 acccagtccg cccggccgtc ggcgttgaga tcgccgacct cggtcaggta agcctcgtca
     2281 tagggatagt cccgacccgc cgcggcgcat cgagccgccc gttcggcgaa cgcggcctcc
     2341 accgcggacg gcagggacgc ccgcgacggc gtcgtcagcc agacggggcc gccgatgagg
     2401 aaggcttgcg cccccggatc gacccgcgcg acgaaccggg cctccgcgac ggaccagaca
     2461 tagccgcgcg gacagtcatc gacgccgaac ccgccgcaga ccgacccgtg gaagtcgaca
     2521 tccagcaggg tctggccccg tttatggcgg agcttgaaca gccggacctg ggtatccatg
     2581 gccaggtcga agccgccccg gggattggac acgaaaatct gctgccgaca gccgccggtg
     2641 ccgcagaaat aggaggggtt gggcgcctgc tcgaagttga cgcgccagtc ggccacgccg
     2701 tcgttgttga cctcgacccg cttgaacatc ttcgggccgg ccagggcgtc aggcggatcg
     2761 tagggttcgc cgctgtcgtc cacgtcgccg ggatcgggcg ccatggcggc gtcgatccgg
     2821 tcctggacag cctgacgaac gctctcgggc ggccggggcg cggcgaaggc cggaccgcca
     2881 aaggtgagcg cgacaatcag tgcgcagacg aaccgcttca aagcctgatc cccgggtccg
     2941 atgaccggcc gcgcggcgaa tcccggcgac cggacaacag aatggtcgcg gcgggcatcg
     3001 gcgcaaaccc gcattcgggg ggcttcggct agagccccgg gtccaccagg atcttcacgt
     3061 tggactccgg attgcccagg tcgacgaagg cctgggcgac gccggcgagc cccaccctgg
     3121 cggtgatcag cgggtcgacg ttgatccgcc cctcggcgat atcgctgagc gtctgggcga
     3181 actcgtcggg ctggtatccg agcacgaacc gcagatcgag ttgcttgttg atcgccagcg
     3241 aaggctcgat ccggtccggc tccatgcaga ccccggcgac gatgatctgg gccttgggcg
     3301 ggcagccctc gatgacgctc tgcagcacgc cgggcgagcc gacgcactcg aacaccacgg
     3361 gccggccgta ggtctggccg ccgaacaggg ccatgcgcat gccgtcgagg gtcgagggca
     3421 cgccgaactc ggcccagcgg gtgtggggac ccgtttctgc cgggtcgacg accacgtcgg
     3481 cgcccatggc ctcggcggcg gcgcggcgac ggggcgagaa gtcggccgcg atgaccgggc
     3541 catggccgcg caccttcagg gcggcgatca ccgccaggcc gaccgggccg cagcccagca
     3601 ccagcgagac ggaattgctg tcgagcgcgg cgctggccac ggcgtgggcg cccacggcca
     3661 tcggctcggt caacgccgcg tggtcggtcg acaggccgtt gggcacttcc agcagcagcg
     3721 cctcggtcag gatcatccgc tcggcgaaac cgcccggata cttgttggaa tagccgacgg
     3781 cgtcgaaccc ttccgggccc atgttgaccg gcatagagac cacccgtgtt cccgccttga
     3841 agcggttcgc cccggggccg ccctcgagca cctcggcgca gaactcatgg ccgaagacga
     3901 tgtcctgctt gggatccatt ccgccgcccg cgccggcgcg gcggcccatc tcgaccatgt
     3961 ggtcgaggta gtgcagggcg tgcaggtccg acccgcagat gccgcaggcc agcgtcttga
     4021 cgcgcacctg gccggggccg ggatcgcgtt cctctatggt gtcgcagacc agtttcccgg
     4081 cgcgccgcac gactgcccgc atggtcgttc tccctgttgt tctgctgcag gctaggcgat
     4141 gccgcaacgg caacgccagc aggaacaaac gcccagccgg ccgacggcca accagcaggc
     4201 ctcccctata tccggctgtt ccggcgaagg cgggaaccca agccgtcctt caggtcgcga
     4261 ctggccccgt gagccgcggt caggggcttg gatccccgcc ttcgcgggga caatcggtga
     4321 ggaagtgcaa cgccagcgac aaccgcgccg atcaggagag ccgggcggcg atggcttcga
     4381 cggtctcgaa cacgtgatcg ggcttctgcg ccttcagggc gtccggggcg gtgtagcccc
     4441 agcagaccac gccggtggcc aggccgacct cgcgggcggc gtcgaggtcg cggatttcgt
     4501 cgccgacgct gatcgcctcc ccggggcgga cctccaggcg tttgagcacc ttgcgaaact
     4561 tgggggcctt gccgaacatc gaggcgccgc agttgaacat gctgaccagc ccgcacaggt
     4621 ccggccccag gatgatgcgg acgttctcct cggagttgga gctgacgatg gccagcctga
     4681 cgccggcccc gtgcagggcg cggagcatct cgggcacgcc gtcgaacatg gcgatgcggc
     4741 ccgcgtcggc ggtcatcagc ttctggaagt gggcggcgat cgccggcagc ttcaccgctg
     4801 gcagcttctg caccttcatc atctcgcggg cgctcagatg gcgcagctgg tccagctggc
     4861 tgcggtcgat cttccggtag ccgaatttct cggccagggc gtcggatgta tccagcatcc
     4921 agccaagggt gtcgcccagg gtgccgtcga agtcgaatat ggccagtctg taggcgggca
     4981 atgaaaatca tcccaatgcg gagcggacaa aaccgggaag gttgcccgtt gtcccccttt
     5041 cccctaaccg tgcgcgccgg attcgtcact ttttcggaga ggtcatcatg agcctcgcct
     5101 tcatctttcc gggccagggc agccagaccg tcggcatggg ggccgagctg gccgaggcct
     5161 tcgccagcgc ccgcgaggtg ttccaggagg tcgacgacgc cctgtcgcag aagctgtcga
     5221 cgctgatgcg cgaggggccc gaggccgatc tgaccctgac cgagaacgcc cagccggccc
     5281 ttatggcggt gagcatggcc gtcatgcgca cgctcgaaag ggaaatgggc gtgccggtcg
     5341 agaaggctgc cttcgtcgcc ggccattcgc tcggcgagta cgccgccctg gccgccgccg
     5401 gggcgattag cctggccgat acggcccggc tgctcaagct gcggggccag gccatgcagc
     5461 gggcggttcc ggtggggcag ggggccatgg cctcgctgat cgggccgaaa acggacgtcg
     5521 ccctggccga ggccgccgcc gcggcgggcg cggaggtcgg ggtctgcgtg gtggccaacg
     5581 acaacaacaa cggcaacgtg gtcatttccg gcgaccgcgc cggggtcgac cgggccatcg
     5641 agaaggcaaa ggaactgggc gcccgggcga ttccgctgaa cgtctccgcc ccgttccact
     5701 gcccgctgat gcagccggcc gctgacgaga tggccgcggc cctggccggc acgaccatcc
     5761 tcgccccgcg cgcgccgctc gtggccaacg tcacggcccg tccgacgctg gatccggagg
     5821 ccatccgccg catgctggtc gagcaggtca ccggacgggt gcgctggcgc gagagcatgg
     5881 aatggctggc cggggagggc ggcgttaccc gcttcgccga ggccggcgcg ggcaaggtgc
     5941 tgtccggcat ggccaagcgc atcgcgccgg acgccgaggc gaccccgctg aacacgccgg
     6001 ccgatctgga agcattcgcg aagagtctgt agcccccgat ccgctcatcc cggctgaggc
     6061 cgggacccag gcgttttccc aaggcgcccg gcgactgcgg atagcaccat aaacctgggt
     6121 cccggccttc gccgggatga gcgggagaag agaatgttcg acctcaccgg caagaccgcc
     6181 ctcgtcacgg gcgccacggg cggcatcggc ggcgccatcg cccgggccct gcacgcccag
     6241 ggcgcgcatg tcgtcctgtc cgacacacgc gagacggcgc tcgag
//
LOCUS       DAORNX010000133         6219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9349_length_6219_cov_6.666450, whole genome shotgun sequence.
ACCESSION   DAORNX010000133 DAORNX010000000
VERSION     DAORNX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9349_length_6219_cov_6.666450"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLO65_07360"
     CDS             complement(<1..582)
                     /locus_tag="PLO65_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRD28101.1"
                     /translation="MSDLTFNKIAGGLLATGLVIFGLRELTTIVFEQEPPEKAGYAVA
                     VAEDTGGGGPAAEVAPDWGTVIPVTNPEVGKAVSAKCASCHTFDAGGANGTGPNLFGV
                     LGRKPGSHAGFAYSPGMMEYAGAHPVWTWDELDQFVKAPQKHIAGTKMTFVGLKKQED
                     RIAMLAYLHTLGSTLPLPAPKPAAAPTEAPAPDA"
     gene            752..1489
                     /locus_tag="PLO65_07365"
     CDS             752..1489
                     /locus_tag="PLO65_07365"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRD28102.1"
                     /translation="MNPIVLIPARMAATRLPDKPLADIGGLPMIVRVLRQAEAAGVGP
                     VVIAAGDQAIVESVAAAGGRAVLTDPDLPSGSDRILAALAAVDPAGAHDVVINLQGDM
                     PFVAAGVVAACAGLLAREPGCDVATVVAPEASPADRSNPDVVKAVLAMAPDGARGRAL
                     YFTRSTLYGDGPVWRHVGIYGYRRAALERFTAAPPSLLERREKLEQLRGLEMGLSYWA
                     AVTDAAPISVDNPADLEEARAFAKANP"
     gene            1486..2334
                     /locus_tag="PLO65_07370"
     CDS             1486..2334
                     /locus_tag="PLO65_07370"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRD28103.1"
                     /translation="MSPLPKIAFQGEPGANSHEACRTYFPDHQAAPYPTFEEAFDAVK
                     SGACALGMIPVENSIAGRVADVHHLLPSSGLKIIGERFKPIRFQLMVNKGVTLDQVKT
                     VASMAIALHQCRGSLKKLGVTTEAVGDTAGAARALAQHPDPTRGAIAPALAAEIYGLD
                     ILLRDIEDERHNTTRFLVMTADKAPPPPPFTSRCITSFCFKVKNIPAALYKAMGGFAT
                     NGVNMTKLESYMEGGAFTATFFYAEVDGRPEDRGLAMALEELSFFSEKLEILGVFPAD
                     PFRDRV"
     gene            complement(2348..2893)
                     /locus_tag="PLO65_07375"
     CDS             complement(2348..2893)
                     /locus_tag="PLO65_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRD28104.1"
                     /translation="MACVVALASAGVAVAGDPFEDARYFMGVKDNAAAIRLIDSGQFD
                     INMQTPEGYTLLHYAADNGNLEMVRELIRRGADVNIRSASDSTPYDMAVGTMVQAEIA
                     RAGGRSGRSPAPSPIPQGTSARAASSTPATGKPASAPVDPRRKMCNARNYAASALCSD
                     STCKMREYRKWQTCLKTGSYY"
     gene            2892..3017
                     /locus_tag="PLO65_07380"
     CDS             2892..3017
                     /locus_tag="PLO65_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28105.1"
                     /translation="MTRNRIRTTNPCKLPGVPYRRSEGSSDGYPTQTLRITPFAV"
     gene            3052..3489
                     /locus_tag="PLO65_07385"
     CDS             3052..3489
                     /locus_tag="PLO65_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD28106.1"
                     /translation="MIQGIPYLQSVLAPVLALIVWSLLIWVWMYAKRIPAMSKAGVKP
                     QDARFPGSLSVLPDDARAAADNYNHLMEQPTIFYALAFVTYLLSLQSPATAGLAWAYV
                     GLRVVHSLVQITVNLVPVRFLVFALSTVVLMVWTGCVVLGVMG"
     gene            complement(3658..4587)
                     /gene="nudC"
                     /locus_tag="PLO65_07390"
     CDS             complement(3658..4587)
                     /gene="nudC"
                     /locus_tag="PLO65_07390"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRD28107.1"
                     /translation="MALSAISNVFSGNPLNRVSERRTDQAWLDGLLADAGSLALPLWN
                     GQPLTEKGGDGGTQLAYVPAHMGLELAGGWERLLFLGLWKETAVFAVDMEGGLDPCDG
                     PLAGLGAFKDLRAVATALPPAEAAITATAKGMFEWRRKHRHCAACGEQSEVAEGGWKR
                     VCISCNAEHFPRTDPVVIMLATFEGRCLLGRQAMWPKGMHSALAGFLEPGETIEEACA
                     RELMEEAGLTATRVHYHSTQPWPYPNSLMIGLIAEVDSADARPDQTELEAVRWFTREE
                     ARQLINGGFDGCFAPPPLAIAHQLVKSWAEGTD"
     gene            4754..4852
                     /gene="ffs"
                     /locus_tag="PLO65_07395"
     ncRNA           4754..4852
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLO65_07395"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4919..>6219
                     /locus_tag="PLO65_07400"
     CDS             4919..>6219
                     /locus_tag="PLO65_07400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRD28108.1"
                     /translation="MADSDISAEPPPWEDHLPERDPDTADIFGAPEPAPEVTPAPAPP
                     EGVAEGQAPGDGAAYTVLARKYRPRTFEDLIGQEAMVRTLTNAFASGRIAHAFMLTGV
                     RGVGKTTTARLLARALNFESDTVHQPSVDLSVDGRHCRAIVEGRHMDVLELDAASRTK
                     VDEMRELLDGVRYAPTEARYKVYVIDEVHMLSTAAFNALLKTLEEPPPHAKFIFATTE
                     IRKVPVTILSRCQRFDLRRVEPEVLVGHLGRIAEAEDARIDAEGLALISRAAEGSVRD
                     GLSLLDQAIVQAERGQVVSAAVVRDMLGLADRALTIALFEQIMGGQTAAALQQYRDLW
                     GFGVDPAQVMLDLLEHNHGASVAKALGPDALHLPKDQAARLAAIGATASAGTLSRVWQ
                     MLLRAYEEVRRAPDPSATVEMALIRLCYAADLPGPEEALKAL"
BASE COUNT          918 a         2208 c         2112 g          981 t
ORIGIN      
        1 ggcgtccggc gccggagcct cggtcggagc cgcggcgggc ttcggagccg gcagcggcag
       61 ggtcgagccc agcgtgtgca ggtaggccag catggcgatg cggtcttcct gcttcttcag
      121 gccgacgaac gtcatcttgg tgcccgcgat atgcttctgc ggcgccttca cgaactggtc
      181 cagctcatcc caggtccaga ccgggtgggc gccggcgtat tccatcatgc cgggcgaata
      241 ggcgaagccg gcatgcgacc cgggcttgcg gcccagcacg ccgaacaggt tcgggccggt
      301 gccgttggcg ccgccggcgt cgaaggtgtg gcaggaggcg cacttggccg agacggcctt
      361 gccgacctcg gggttggtca ccggaatcac cgtgccccag tcgggcgcga cctcggcggc
      421 cgggccaccg ccgcccgtgt cctcggccac cgccacggcg tagccggcct tttccggcgg
      481 ctcctgctcg aaaacgatgg tggtcagctc acgcaggccg aaaatgacga gtcccgtcgc
      541 cagcaatccg ccggcgatct tgttgaacgt cagatcgctc atgtccgcct gtctcagtcc
      601 tctagaccgc ccggcgcgcc gggcctgaaa cggcgccggg agaatccccc gacgcgcccc
      661 ttgttgcgcc gggctctctt acacgctacc gcccctcgcg caagccgggc tcaggggtcg
      721 gctggcccga attgatccac ggcgcagagt catgaacccg atcgtcctta tccccgcgcg
      781 catggcggcc acccgcctgc ccgacaaacc gctggccgat atcggcggcc tgccgatgat
      841 cgtgcgggtg ctgcggcagg cggaagcagc cggcgtcggc cccgtggtca tcgctgcggg
      901 cgaccaggcg atcgtcgagt ccgtcgccgc cgccggcggg agagccgtcc tgaccgaccc
      961 tgacctgccc tccggctccg accgcatcct ggcggccctt gcggcggtcg acccggccgg
     1021 cgcccatgac gtggtgatca acctgcaggg cgacatgccg ttcgtcgcgg ccggcgtggt
     1081 cgccgcctgc gccggcctgc tggcgcgcga acccggctgc gacgtggcca cggtggtcgc
     1141 gccggaggcg tctccggccg acagatcgaa ccccgacgtg gtcaaggccg tgctggcgat
     1201 ggcgcccgac ggggcccgcg gccgggcgct ctatttcacc cgctcgaccc tctacggcga
     1261 cgggcccgtc tggcggcatg tcggcatcta cggttatcgc cgcgcggcgc tggagcgatt
     1321 taccgccgct ccgccctcgc tcctcgaacg gcgcgagaag ctggagcagc tgcggggcct
     1381 ggagatgggc ctttcgtact gggccgccgt cacggacgcc gcgcccattt ccgtggacaa
     1441 ccccgccgac ctcgaggagg cgcgggcatt cgccaaggcc aatccatgag cccgcttccc
     1501 aagatcgcct tccagggcga acccggcgcc aacagccacg aggcctgccg gacctatttc
     1561 cccgaccacc aggccgctcc ctatccgacg ttcgaggagg ccttcgacgc ggtgaagtcc
     1621 ggggcctgcg ccctgggcat gatcccggtc gagaactcca tcgccggccg ggtggccgac
     1681 gtacatcatc tgctgccgag ttcgggcctg aagatcatcg gcgagcggtt caagccgatt
     1741 cgcttccagc tgatggtcaa caagggcgtg acgctggacc aggtgaagac cgtcgccagc
     1801 atggccatcg ccctgcacca gtgccgggga tcgctgaaga agctgggcgt gaccaccgag
     1861 gccgtcggcg acaccgccgg cgcggcccgg gccctggccc agcaccccga tccgacccgc
     1921 ggggccatcg ccccggcgct ggcggccgaa atctacggcc tcgacatcct gctgcgcgac
     1981 atcgaggacg aacggcacaa caccacccgt ttcctggtca tgaccgccga caaggccccg
     2041 cctccgccgc cgttcaccag ccgctgcatc accagcttct gcttcaaggt gaagaacatc
     2101 ccggcggccc tctacaaggc catgggcggg ttcgcgacca acggcgtcaa catgaccaag
     2161 ctggaaagct acatggaagg cggcgccttc acggcgacct tcttctacgc cgaggtcgac
     2221 ggtcggcccg aggaccgggg cctggccatg gcccttgagg agctgagctt cttctccgag
     2281 aagctcgaga tcctcggggt gtttccggcc gatccgttcc gggaccgggt ctgacctcca
     2341 gtcgcggtca gtagtagctg cccgtcttca ggcaggtctg ccatttgcgg tattcgcgca
     2401 tcttgcaggt gctgtcgctg cacagggccg aggctgcgta gttcctcgcg ttgcacatct
     2461 tgcgacgtgg atcgaccggg gcggaagcgg gtttgccggt ggcgggggtc gaagacgcgg
     2521 cgcgggccga agtcccttgc gggatcgggg atggcgcggg cgagcggccg cttcggccgc
     2581 cggccctggc gatctcggcc tggaccatcg tcccgaccgc catgtcgtac ggagtcgagt
     2641 ccgacgccga gcggatatta acgtcggcgc cccggcggat gagctcacgg accatctcga
     2701 gattgccgtt gtccgccgcg tagtgcagca gcgtgtagcc ttccggggtc tgcatgttga
     2761 tgtcgaactg gccgctgtcg atcagccgga tcgccgcggc gttgtccttc acgcccatga
     2821 aatagcgggc atcctcgaag ggatctcccg ccaccgcgac gccggccgag gccagcgcga
     2881 cgacacaggc catgacacgc aacagaatcc gcacgaccaa cccctgcaag ctgcctggcg
     2941 tcccctaccg ccgttcggaa ggatcgtctg acggctatcc gacacagacc cttcgcatca
     3001 cgccgttcgc ggtctagcct ccggcgcaat caaccgcctg gggaggcaac cgtgattcaa
     3061 ggcattcctt acctgcagtc cgtcctggcg ccggttctgg cgctgatcgt ctggtccctg
     3121 ctgatctggg tctggatgta cgccaagcgc atcccggcca tgagcaaggc cggggtcaaa
     3181 ccgcaggacg cgcgcttccc gggttcgctg agtgtgctgc ccgacgacgc ccgggcggcg
     3241 gccgacaact acaatcacct gatggaacag ccgacgatct tctatgcgct ggccttcgtg
     3301 acctatctgc tgagcctcca gagcccggcc accgccggcc tcgcctgggc ctatgtcggc
     3361 ctgcgcgtgg tgcacagtct ggtgcagatc acggtcaacc ttgtgccggt gcggttcctg
     3421 gtcttcgcgc tgtcgaccgt ggtgctgatg gtctggaccg ggtgtgtggt gctgggggtg
     3481 atggggtagc cctatttcct tcctcccctc gcagagggga gggggaccat gccgagcatg
     3541 gtggaggggc agcgccggcc aagccggaaa gaccggaatt caggataggg cccttcgctt
     3601 ttgcgccgcc cctccaccgc ctccggcggt ccccctcccc gcgagcgggg aggaaaacta
     3661 gtccgttccc tccgcccagc tcttgaccag ctggtgggcg atggccagcg ggggcggggc
     3721 gaagcagccg tcgaagccgc cgttgatcag ctgccgggcc tcctcgcggg tgaaccagcg
     3781 gacggcctcc agctcggtct ggtcgggccg ggcgtcggcg ctgtcgacct cggcgatcag
     3841 gccgatcatc agcgagttgg ggtagggcca gggctgggtc gagtggtagt ggacgcgcgt
     3901 cgccgtcagc ccggcctctt ccatcagctc gcgggcgcag gcctcctcga tggtctcgcc
     3961 gggctcgagg aaaccggcca gggccgaatg catgcccttg ggccacatcg cctggcgtcc
     4021 gagcaggcag cggccctcga acgtggccag catgatcacc accgggtcag tgcgtggaaa
     4081 gtgctcggcg ttgcaggaaa tgcagacccg cttccagccg ccctcggcga cctcgctctg
     4141 ttcgccgcag gcggcgcagt ggcgatgctt gcggcgccat tcgaacatgc ccttggcggt
     4201 cgccgtgatg gccgcctcgg ccgggggcag ggccgtggcc acggcccgca ggtccttgaa
     4261 cgcgcccaac cccgccaggg gcccgtcgca cggatcgagc ccgccttcca tgtcgacggc
     4321 gaacacggcc gtctccttcc acaggccgag gaacagcagc cgctcccagc cgccggccag
     4381 ttccaggccc atgtgcgccg gaacgtaggc cagctgggtc cccccgtccc cgcccttctc
     4441 cgtcagcggc tggccgttcc acagcggcag ggccagcgac ccggcgtcgg ccagcaggcc
     4501 gtcgagccaa gcctggtcgg tccgtcgttc gctgacacgg ttcagcgggt tgccggagaa
     4561 gacattggag atcgcggaga gagccatggc ggcttcgtgg cgcaaacggc ggtttcgggc
     4621 aagcactgac cggggacatg taccttcagt tacgtgtccc ccaccctggt tgctcgaaca
     4681 ggccacacgc accggaggtg catgtcacct gccggcgcct tgcatccgcc ccgccgatcc
     4741 acgatatagg ccttggagac cggcgatggg cggacgcctc gccaacctgg tcagggccgg
     4801 aaggcagcag ccacaacgag atttgctccg ggtcgttgtc cggtctccac ccttcccata
     4861 ggttcttttg ctgttctccg ccttcgcggg gatgagcggg tgtgagacga tagggtcgat
     4921 ggccgattcc gatatctccg ctgaaccgcc gccctgggag gaccacctcc ccgagcgcga
     4981 tcccgatacc gcggacatct tcggcgcgcc cgaaccggcc ccggaggtca cgccggcccc
     5041 ggccccgccg gaaggcgtcg ccgagggcca ggccccgggc gacggcgcgg cctacaccgt
     5101 gctggcccgc aaataccggc cgcgaacctt cgaggacctg atcggccagg aggccatggt
     5161 ccggaccctg accaacgcct tcgccagcgg ccgcatcgcc cacgccttca tgctcaccgg
     5221 ggtgcgcggg gtcggcaaaa ccacgaccgc ccgcctgctg gcccgggcgc tcaatttcga
     5281 gagcgacacc gtgcaccagc cgtcggtcga cctttccgtc gacggacgcc attgccgggc
     5341 gatcgtcgag ggccggcaca tggatgtgct ggagctggac gccgccagcc gcaccaaggt
     5401 cgacgagatg cgcgagctgc tcgacggggt gcgctacgcc ccgaccgagg cgcgctacaa
     5461 ggtctatgtc atcgacgagg tgcacatgct gtcgacggcg gcgttcaacg cgctgctgaa
     5521 gacgctggaa gagccgccgc cgcacgccaa gttcatcttc gccaccaccg agatccgcaa
     5581 ggtgccggtg accatccttt cccgttgcca gcgcttcgac ctgcgccgcg tcgagccgga
     5641 ggtgctggtg ggccacttgg gccgcatcgc cgaggccgag gacgcccgca tcgacgccga
     5701 gggcctggcg ctgatctccc gggcggccga gggctcggtg cgcgacggcc tgtcgctgct
     5761 cgaccaggcc atcgtccagg ccgaacgcgg gcaggtggtc agcgccgcgg tggttcgcga
     5821 catgctgggc ctggccgacc gggccctgac gatcgccctg ttcgagcaga tcatgggtgg
     5881 ccagaccgcc gcggccctgc agcagtatcg cgacctctgg gggttcggcg tcgacccggc
     5941 ccaggtgatg ctcgatctgc tcgagcacaa tcacggcgcc tcggtggcca aggcgctggg
     6001 gcccgacgcc ctgcacctgc cgaaggacca ggccgcgcgg ctggcggcga tcggggccac
     6061 ggcgtcggcc ggcaccctgt cgcgggtctg gcagatgctg ctcagggcct atgaggaggt
     6121 gcggcgggcg cccgatccct cggccacggt cgagatggcg ctgatccgcc tgtgctacgc
     6181 ggccgacctg ccgggaccgg aggaggcgct gaaggccct
//
LOCUS       DAORNX010000134         6196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9395_length_6196_cov_8.977040, whole genome shotgun sequence.
ACCESSION   DAORNX010000134 DAORNX010000000
VERSION     DAORNX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9395_length_6196_cov_8.977040"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLO65_07405"
     CDS             <1..1130
                     /locus_tag="PLO65_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28109.1"
                     /translation="EMADAGAAPAAPPLAGANALNPGISVVLNGNYVASSRDPDLARI
                     PGFALGDEAGLAPRGFSLAESEVTLAANVDPYLYANLTVAFDGQGEAGVEEAYIRTTA
                     LPAGLTLKGGRFFSGIGYLNERHAHNWSFIDMPLPYRAFLGGQYGDDGVQAVWLAPTA
                     MFLEIGAELFRGDSFPAGNGADRGAGTQTVFVHTGGDISDSSSWLGAASWIHTRADAR
                     ETSDPVAGIDVFSGDTDLGIISGVYKWAPGGNSTQRNLALSGEYFFGTRSGEFNGVPV
                     EADQNGWYVQGVYQFRPRWSFGLRYSTVEAGDVPLPLAGSTLDDLGVSSWSASALLEY
                     DSSEFGRFRLQYSRDESDQRPRDELFLQYTVVYGPHGAHRY"
     gene            1140..2042
                     /locus_tag="PLO65_07410"
     CDS             1140..2042
                     /locus_tag="PLO65_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRD28110.1"
                     /translation="MFRSLVLAAALALGVSTPTWAMNVFACEPEWAALASEIGGPKVT
                     TYTATTALQDPHQIQARPSLIAKARAADITVCTGSELEIGWLPQILTQAANKKIAPGS
                     AGSFEATRYVTLLERPARLDRAEGDIHAGGNPHIQSDPRVMLPVGKAMAERFAQIDPA
                     NAALYRARYAAFAVKWQAALQRWQARAAPLRGQPIAVQHKTWVYLETWLGLKRVVALE
                     PKPGVPASSGYLAQVLGVLQKTPVKMVIRSAYEDGRSSEFVSQRAHVPAVVLPYTIGG
                     SPGAKDLYSLYDDTLTRLLGALGK"
     gene            2039..2836
                     /locus_tag="PLO65_07415"
     CDS             2039..2836
                     /locus_tag="PLO65_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRD28111.1"
                     /translation="MTIGAIELSILLPALLAGLLVLSTHVPMGQQVLNRGIVFIDLAI
                     AQVAGLGVTIASSLGAEPQGWHVQLAACVAALIGALVLTWTEKKWPQVQEALIGVLFV
                     VAACVELLLLANNPHGGEHLKDLLVGQILWVQIPGLIPIAVFYAVALAIWFFLRHRLG
                     QIGFYVLFALVVTQSVQLVGVYLVFASLIVPALASRRFAPKLRLGVGYAVGVVGYVAG
                     LVVSSVLDLPTGAAIVVTLVASFVLAVIASLLVPAGLRAPPEPAPAH"
     gene            complement(2877..3728)
                     /locus_tag="PLO65_07420"
     CDS             complement(2877..3728)
                     /locus_tag="PLO65_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRD28112.1"
                     /translation="MPIAYPEILSLASEPQPFSWTDKDTMLYALGIGMGSDPMNEQEL
                     PFVYESGLKAVPTQATTVAWGAGPGIGKMGINFLLVVHGEQKVEFHKPLPTEASILAT
                     SRVIGAYDKGDKGAVIYNETVLADAKSGEKIATLTGSTFARGDGNFGGPSEGAPEPHE
                     VPTRAPDRSVDITTRPDQALIYRLSGDRNPLHSDPKIAAAAGFPRPILHGLCTYGITC
                     RAVLQEYCDFDPAKIKSHQVRFSSPVFPGETITVDLWKDGEVVSFEARVKSRGVTVVK
                     NGKTQLG"
     gene            3887..4690
                     /locus_tag="PLO65_07425"
     CDS             3887..4690
                     /locus_tag="PLO65_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HRD28113.1"
                     /translation="MEQTKAAIGPVSGNLLFYTSPEPLTKDVHSGLGVQRMEKPFGFA
                     AAGHVVPLTVAEFTFAAISYPIIFAGSEHMPLAVMGINSGENLFINDDGSFVVGGYVP
                     AYIRRYPFVLAADEAQQRMVVCIERNAAIFTDKAKAEMPLFDDAGEPSEYTQNAIKFC
                     EDFEGERRRTDSFVKLLLDLDLFEQRESIFNMPREDGTVQPVKLAEYFAVSEAKLNAL
                     PAEKLAELRDNGALEKIYNHLTSLIGWDRLVAVASERALRRSGETPANA"
     gene            complement(4727..5119)
                     /locus_tag="PLO65_07430"
     CDS             complement(4727..5119)
                     /locus_tag="PLO65_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HRD28114.1"
                     /translation="MPASFDEISIGQVVNLGAVAVDAAALEAFARAFVPGWGVERGAP
                     DAMIYALWSRLEEHASADWPQTKRLGVDALRWARNPPVGELLRGRMTVMGKDPVGEDK
                     GIVIAQHDLLDEAGRLVFSCLTRSVFAR"
     gene            complement(5124..6170)
                     /locus_tag="PLO65_07435"
     CDS             complement(5124..6170)
                     /locus_tag="PLO65_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD28115.1"
                     /translation="MIEHQPGRDDMPVNRQWILKRRPVGEIAPGDLEFVERPLRDLEP
                     GEVLVRNVYLSLDPTNRIWMSDQDQYLPPVEVGDVMRGGTIGVVEVSRSERFPAGTVV
                     NAGLSGWQSYSIAHEGMVSPVPALPGVPLTAYMSVLGATGLTAWFGMVDIGRPQPGET
                     VVVSAAAGAVGSVAGQIARLKGARVIGIAGGPEKCAWLTRDLGFDGAIDYRNEDVGAA
                     LDRLCPNGIDVNFENVGGAIMDAVIARMNNFSRMPLCGMISTYNATGPVPGPSDFARV
                     LMRRILIKGFIVIDYLPRAAEALAELAPWVLEGKIKWKAHAVDGLENAAEAVKLLFSG
                     AHDGKLLVRISAEP"
BASE COUNT          913 a         2206 c         2070 g         1007 t
ORIGIN      
        1 tggagatggc cgacgccggc gccgccccgg ccgccccgcc cctcgccggg gccaatgccc
       61 tcaaccccgg catctcggtc gtgctcaacg gcaactatgt cgcctcctcg agagaccccg
      121 acctggcccg cattccgggg ttcgccctcg gcgacgaggc cggcctggcc ccgcgcggct
      181 tctcgctggc ggagtcggag gtgaccctgg ccgccaacgt cgacccctat ctctacgcaa
      241 acctgaccgt cgccttcgac ggccagggcg aggccggggt cgaggaggcc tatatccgca
      301 ccacggccct gccggccggc ctgaccctca agggcggccg gttcttctcc ggaatcggct
      361 atctgaacga gcgccacgcg cacaactggt cgttcatcga catgccgctg ccctaccgcg
      421 ccttcctcgg cgggcagtat ggcgacgacg gggtgcaggc cgtctggctg gcgccgaccg
      481 ccatgttcct cgagatcggc gcggagctgt tccgcggcga cagcttcccc gccggcaacg
      541 gcgccgatcg cggcgcgggg acccagacgg tgttcgtcca cacgggcggc gacatcagcg
      601 actcctccag ctggctcggg gccgcatcct ggatccacac ccgggcggac gcccgcgaga
      661 ccagcgaccc tgtagcgggc atcgacgtgt tcagcggcga taccgacctt gggatcatct
      721 ccggcgtcta caaatgggcg ccgggcggca acagcacgca gcgcaacctg gcgctcagcg
      781 gcgagtattt cttcgggacc cggagcggcg aattcaacgg cgtgccggtc gaggccgacc
      841 agaacggctg gtatgtccag ggcgtctacc agttccggcc ccgctggagc tttggcctgc
      901 gctactcgac cgtggaggcg ggcgacgtcc cgctgccgct ggccggctcg accctcgacg
      961 acctgggcgt ctcgtcatgg agcgcctcgg ccctgctcga gtacgactcc agcgagttcg
     1021 gccgcttccg gctgcagtac tcccgcgacg aaagcgacca gcggccgcgc gacgaactct
     1081 tcctgcaata cacggtcgtc tacggccccc acggcgccca ccgctactaa ggagcttcca
     1141 tgttcagatc tcttgtcctc gcggcggcgc tggcgctcgg cgtgagcacg ccgacgtggg
     1201 ccatgaacgt ctttgcctgc gaacccgagt gggccgccct ggccagtgag atcggcggtc
     1261 ccaaggtgac cacctacacc gccaccacgg ccctgcagga cccgcaccag atccaggccc
     1321 ggcccagcct gatcgccaag gcccgcgccg ccgacatcac cgtctgcacc gggtcggagc
     1381 tggagatcgg ctggctgccg cagatcctca cccaggccgc caacaagaag atcgcgccgg
     1441 gttcggcggg atccttcgag gcgactcgct atgtcaccct gctcgagcgg cccgcccggc
     1501 tcgaccgcgc cgagggcgac atccacgcgg gcggcaatcc ccacatccag tccgatcccc
     1561 gggtcatgct gccggtcggc aaggcgatgg ccgagcggtt cgcccagatc gatccggcca
     1621 acgcggccct ctaccgcgcc cgctacgccg ccttcgccgt gaagtggcag gccgccctgc
     1681 agcgctggca ggcccgggcc gccccgctgc ggggccagcc gatcgccgtg cagcacaaga
     1741 cctgggtcta tctggagacc tggctgggcc tcaagcgggt ggtggcgctg gagcccaagc
     1801 cgggcgtgcc ggcctcgagc ggctatctcg cccaggtgct gggggtgctg cagaagacgc
     1861 cggtgaagat ggtcatccgc tcggcctatg aggacggccg ctcgtcggag ttcgtctcgc
     1921 agcgcgccca cgtcccggcg gtggtgttgc cctacaccat cggcggcagc cccggcgcca
     1981 aggacctcta cagcctctac gacgacaccc tgacccgtct gctgggagcg ctcggcaaat
     2041 gaccatcggc gcgatcgaac tgtccattct gctgccggcc ctgctggccg gcctgctggt
     2101 gctgtccacc cacgtgccga tgggccagca ggtgctgaat cgcggcatcg tgttcatcga
     2161 cctggccatc gcccaggtgg ctggcctggg cgtgaccatc gccagcagcc tgggcgcgga
     2221 gccgcagggc tggcacgtcc agctggcggc ctgcgtcgcc gccctgatcg gcgccctggt
     2281 gctgacctgg acggagaaga agtggccgca ggtccaggaa gccctgatcg gcgtgctgtt
     2341 cgtggtggcg gcctgcgtcg agctgctgct gctggccaac aacccgcacg gcggcgagca
     2401 tctgaaggat ctgctggtcg gccagatcct ctgggtgcag atccccggcc tgatcccgat
     2461 cgcggtgttc tacgcggtgg ccctggcgat ctggttcttc ctgcgccacc ggctcggaca
     2521 gatcggcttc tatgtgctgt tcgccctggt ggtcacccag tcggtgcaac tggtcggcgt
     2581 ctacctcgtg ttcgccagcc tgatcgtgcc ggctctcgcc tcgcggcggt tcgctccgaa
     2641 actgcggctg ggcgtcggct acgccgtagg cgtcgtcggc tatgtggcgg gactggtggt
     2701 gtcgtcggtg ctcgatctgc cgaccggcgc ggcgatcgtc gtcaccctgg tggccagctt
     2761 cgtcctcgcg gtgatcgcct cgctgctggt cccggccggc ctgcgggccc cgcccgagcc
     2821 cgcgccggct cactgacaga aggggacatg tacccgcggt acatatcccc tgcatcctat
     2881 cccagctgcg tcttgccgtt cttgaccacc gtgacgccac gcgacttcac ccgggcctcg
     2941 aacgagacga cttcgccgtc cttccacagg tcgacggtga tcgtctcgcc cgggaaaacg
     3001 gggctggaga agcgcacctg gtggctcttg atcttcgcgg gatcgaagtc gcagtactcc
     3061 tggagcacgg cgcggcaggt gatgccgtag gtgcaaaggc cgtgcaggat cgggcggggg
     3121 aagccggccg ccgcggcgat cttcggatcc gaatgcaacg gattgcggtc gccactcagc
     3181 cggtagatca gcgcctggtc gggccgggtc gtgatgtcca cggaccggtc cggcgcgcgc
     3241 gtcggcacct catgcggctc cggggcgcct tcggaaggcc cgccgaaatt cccgtcgccc
     3301 cgggcgaagg tcgagccggt cagggtggcg atcttctcgc cggacttcgc gtcggccagc
     3361 acggtctcgt tgtagatcac cgcgcccttg tcgcccttgt cataggcgcc gatcacccgg
     3421 ctggtggcca ggatgctggc ttcggtgggc aggggtttgt gaaattcgac cttctgctcg
     3481 ccgtgcacga ccagcaggaa gttgatgccc atcttgccaa tcccggggcc cgctccccag
     3541 gcgaccgtgg tggcctgggt cggaaccgcc ttcagcccgc tttcgtagac gaacggcagc
     3601 tcctgctcgt tcatcgggtc cgagcccatg ccgatcccca gggcgtagag catggtgtcc
     3661 ttgtcggtcc agctgaacgg ctggggctcg ctggcgagcg acagaatctc tgggtaggcg
     3721 atgggcatgg acggctccgg gggttttctt cttgtggtga ttgaagcgcg ttcgcccggg
     3781 ggaccgcaag acgggagctc ggattgccgg ggtccctttg atcggctaga agcgccggct
     3841 gatcttcatc gcggccgcat cccgtccgca gcttcaagag tctcgcatgg aacagaccaa
     3901 agccgccatc ggccccgtca gcggcaacct gcttttctac acctcgccgg agcccctgac
     3961 gaaggacgtg cacagcggcc tgggcgtgca gcggatggaa aagccgttcg gcttcgccgc
     4021 cgccggccat gtggtgcccc tgacggtcgc cgagttcacc ttcgcggcga tcagctaccc
     4081 gatcatcttc gccggctcag agcacatgcc gctggcggtg atgggcatca actccggcga
     4141 gaacctgttc atcaacgacg acggctcgtt cgtcgtcggc ggctatgtgc cggcctacat
     4201 ccgccgctac ccgttcgtgc tggcggctga cgaagcccag cagcggatgg tcgtctgcat
     4261 cgagcgcaac gcggcgatct tcaccgacaa ggcgaaggcc gagatgcccc tgttcgacga
     4321 cgccggcgag ccgtcggaat acacccagaa cgccatcaag ttctgcgagg acttcgaggg
     4381 cgagcgccgc cggaccgact cgttcgtcaa gctgctgctc gacctggatc tgttcgaaca
     4441 gcgcgagtcg atcttcaaca tgccccgcga ggacggcacc gttcagccgg tcaagctggc
     4501 cgagtacttc gccgtctccg aagccaagct caacgccctg ccggccgaga agctggccga
     4561 gctgcgtgac aacggcgcgc tggagaagat ctacaaccac ctgacctcgc tgatcggctg
     4621 ggaccgcctg gtggcggtgg ccagcgagcg cgccctgcgc cgctcgggcg agaccccggc
     4681 gaacgcctga tcgacccggg ccgcggtcgc ccccggcggt cgcggcctat cgggcgaaga
     4741 cgctccgcgt caggcaggaa aagaccaggc ggccggcctc gtcgagcagg tcgtgctggg
     4801 cgatgacgat gcccttgtcc tcgccgacag gatccttgcc catgaccgtc atgcggccgc
     4861 gcagaagctc cccgaccgga gggttgcggg cccagcgcag ggcgtcgacc cccaggcgct
     4921 tggtctgggg ccagtcggcc gaggcgtgct cctcgagccg cgaccagagg gcgtagatca
     4981 tcgcgtccgg cgcgccgcgc tcgacgcccc agcccgggac gaaggcgcgg gcgaaggctt
     5041 ccagggccgc cgcgtccacc gctaccgcgc caaggttgac cacctggccg atcgagatct
     5101 cgtcgaagct ggcgggcatc gcttcagggc tccgccgaga tgcgcacgag cagcttgccg
     5161 tcatgggcgc cgctgaacag cagcttgacc gcctcggcgg cgttttccag cccgtccacg
     5221 gcgtgggctt tccacttgat cttcccttcg agcacccagg gcgccagctc cgccaaggcc
     5281 tcggcggcgc gcggcaggta gtcgatgacg atgaagccct tgatcaggat ccgccgcatc
     5341 agcacccggg cgaagtcgct cggcccgggc acggggccgg tggcgttgta ggtgctgatc
     5401 atgccgcaca ggggcatgcg gctgaagttg ttcatgcggg cgatgacggc gtccatgatc
     5461 gccccgccga cgttctcgaa attgacgtcg ataccattgg ggcacagccg gtccagcgcc
     5521 gccccgacat cctcgttgcg gtagtcgatg gccccgtcga atccgaggtc gcgggtcagc
     5581 caggcgcact tctccggccc gccggcgatg ccgatgaccc gggcgccctt gagcctggcg
     5641 atctggccgg ccacggagcc gaccgccccg gcggcggcgg acacgaccac cgtctcgccc
     5701 ggctgcggcc ggccgatgtc gaccatgccg aaccaggccg tcagacctgt ggcgccaagc
     5761 acgctcatgt aggccgtcag cggcacgccg ggcagggcgg ggaccggact gaccatgccc
     5821 tcatgggcga tggagtagct ctgccagccc gacagcccgg cgttgaccac cgttcccgcc
     5881 ggaaaccgct ccgagcggct gacctcgacc acgccgatgg tcccgccgcg catcacatcc
     5941 ccgacctcga ccggcggcag gtactggtcc tggtcgctca tccagatgcg gttggtcggg
     6001 tccagcgaca gatagacgtt gcgcaccagg acctcaccgg gctccagatc gcgcagcggt
     6061 cgctcgacga attccaggtc ccccggggcg atctcgccga ccgggcgtcg cttgagaatc
     6121 cactggcgat tgaccggcat gtcgtccctc cctggctggt gttcgatcat ggcgaccccg
     6181 tcgccgttgt cgaggg
//
LOCUS       DAORNX010000135         6196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9396_length_6196_cov_7.452695, whole genome shotgun sequence.
ACCESSION   DAORNX010000135 DAORNX010000000
VERSION     DAORNX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9396_length_6196_cov_7.452695"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1072)
                     /locus_tag="PLO65_07440"
     CDS             complement(146..1072)
                     /locus_tag="PLO65_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRD28116.1"
                     /translation="MAHDDHGHHGHSHDHGHGHGHGHGHSHAPADFGRAFAIGAALNI
                     GFVAVEALAGLLTGSLALMADAGHNLSDVLGLLMAWGALVLARALPTSRRSYGLRKGT
                     ILASLANAGLLLLAVGAIVWEAIRRLGTPEPLSPGPVMIVAVIGVLINTATALMFMRG
                     QHDLNVRGAFLHMAADAAVSLGVVLAAAVIWLVPAWTWIDPLVGLVIAAVIVAGTWGL
                     LRDSLDLALDAAPRGVDPDAVRDWLAARPGVSTTETALTAHLTRPDNADGDAFLHDTA
                     LALSERFRIGHPTLQIETGAGGTCRLAPVDVV"
     gene            complement(1086..1787)
                     /locus_tag="PLO65_07445"
     CDS             complement(1086..1787)
                     /locus_tag="PLO65_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD28117.1"
                     /translation="MKAFFRSAFVQQTLASLFAAWLRLCYATVRWSRQDQARVEAVWA
                     TEGGAILCFWHAVIPLAPPSWPQSPHRQDMRALISRSADGEFIALTVQKIGFPAIRGS
                     SKKVTDAAKNKHGEQALRDMVKWVRSGGGVAITPDGPRGPAEVMQGGAPTLARMTGAP
                     VLFLGLAIRPCLRIGSWDRTIVPLPFGRGAMVWDGPVFASRSDDLEALAADWTGRLSA
                     AQRRAEALVDAAGRQ"
     gene            complement(1784..3559)
                     /locus_tag="PLO65_07450"
     CDS             complement(1784..3559)
                     /locus_tag="PLO65_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD28118.1"
                     /translation="MTAAAPAEMTDRQLAARVWRTYLRRRWRGVTLALLGAIGVAVFS
                     TLLAQMIEPAVNDLLGRSPKPGAIVIIPLTIIAYGLGRAASQIVQAMLVNVIGHGLVG
                     DIQQELFGRMVRADLARLRATHSGSFVSQVLYDAALIREAATSGVVNWTQNLLIVGGC
                     LVAMIDRDPVLAAGVALVGPFAGRLMRRFSRRSKKAAKGAMAATSDLSTALMESLDGV
                     RVVKMENREADEEARVAAVIDARQKFIIKGANARAQSAPVTELLTVFVTAAIFVYVGW
                     RHNMNPGDFMAFFALLGMAGQSLRQLANLQTVFAEGMTAARRLFLALDVEPEVRESVS
                     AHALVAGDGEVRLEDVGFAYDDGGAILDGVTIEARRGEMVALVGPSGGGKSTILSLIP
                     RFYDATSGRVTVDGSDVREVTIASLRARIAFVTQEPFLFDDTIRANIAYARPEATDAE
                     IEEAARRAAAHDFILEQPDGYDTVVGEAAARLSGGQRQRIAIARAFLKNAPILLLDEA
                     TSALDTESEAQVQEALDRLMAGRTTIVIAHRLSTVKHADRIYVIDRGRVVESGAHASL
                     VRRRGLYARLARAQNLDTAPEETPA"
     gene            3639..4307
                     /locus_tag="PLO65_07455"
     CDS             3639..4307
                     /locus_tag="PLO65_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HRD28119.1"
                     /translation="MSKRFDLSTGWGRFKAYWHSIWGDHAFLRVGFSNAHWIGPDMVR
                     AAQPWPYQLKYWKRDGIRTIVNLRGGFDTGFYVLEREACERLGLTMVDFTITSREVPR
                     RETVLGARDLFQSIEYPALMHCKSGADRAGIMSVFYRHFHLGEPIREAMKELGLRTLH
                     VKQGKTGVLDYVFERYLAEAEPKGISFIDWVQSDAYDRKQIKDDFKASWWGTLLTDRL
                     LRRE"
     gene            complement(4395..4631)
                     /locus_tag="PLO65_07460"
     CDS             complement(4395..4631)
                     /locus_tag="PLO65_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRD28120.1"
                     /translation="MPDRQLLHIVIGGELKDVQDVEFRDLSKVEFVGAYPNYAEAWKA
                     WKGKAQATVDNAHARYFIIHAHKLLDPNEDDDQH"
     gene            complement(4659..5444)
                     /locus_tag="PLO65_07465"
     CDS             complement(4659..5444)
                     /locus_tag="PLO65_07465"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRD28121.1"
                     /translation="MKDDLALIVETAREAGDLARRMKGEGLKIWSKTGGSPVTDADIA
                     VDQLLKARLEAARPDYGWLSEETADDAARLDRRRLFVVDPIDGTAAFLKNRPWWSVSI
                     AVVDAGRPTAGVVFAPELDETFTATAGGGAFLNDTAIRASEQARVEDCRMLGDAKMFA
                     HPAWREPWPPMTIETRNSIAYRMCLVADGRFDAALALSGKSEWDLAAADLICAEASAV
                     CTDHKGRGYRYNQPSPRLPSLISAAPALHPLILERVRHIDLPN"
     gene            complement(5441..>6196)
                     /locus_tag="PLO65_07470"
     CDS             complement(5441..>6196)
                     /locus_tag="PLO65_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRD28122.1"
                     /translation="RTVRHGADLPSPTAIGAEAGKRAVQRLGARKIDSITAPVIFENR
                     LAISLIGPLIGAISGPSVARGVSFLKDRLGQPLFSRGVQIVDDPHRVRGLGSSPFDDE
                     GVANRRTAIIEDGVLTTWLLNTATAKQLGMQTTGHASRGLAGPPGVSTTNLTLEPGER
                     DLAGLMADAGDGLLITSMFGPSLNANTGDWSVGCSGFWFEKGQIAYPVTEITVAGNLI
                     DIWGRLVPGSDLEVRGAANAPSLLVDAMPIAGR"
BASE COUNT          951 a         2122 c         2220 g          903 t
ORIGIN      
        1 ccgtggaccc gccatagacc acacttccga ggtacttgac gccggaggtg aggccgctga
       61 agctgaggct gaccgtgccg gattggccga gcacggccgt ggccgcgcgg tagagcatca
      121 gggatccggg cagcgccacg ccggctcaga ccacgtcgac gggcgcgagg cgacaggtcc
      181 cgccggcgcc ggtctcgatc tgcagggtcg gatggccgat gcggaagcgt tcggacaggg
      241 ccagggctgt gtcatgcagg aaggcgtcgc catcggcgtt gtccggccgc gtcaggtgcg
      301 cggtcagggc ggtctcggtg gtgctcacgc ccggccgggc ggccagccag tcgcgcaccg
      361 cgtcagggtc cacgccgcgc ggcgcggcgt ccagcgccag gtcgagcgag tcgcgcagca
      421 ggccccaggt ccccgcgacg atgaccgcgg cgatgaccag gccgaccagc gggtcgatcc
      481 aggtccaggc cggaaccaac cagatgaccg ccgcggccaa caccaccccc agcgagaccg
      541 ccgcgtcggc agccatgtgc aggaaggcgc cccgcacgtt gaggtcatgc tggccgcgca
      601 tgaacatcag cgccgtggcg gtgttgatga gcacgccgat gacggcgacg atcatcaccg
      661 gccccggcga cagcggctcg ggcgtcccca gccggcggat ggcctcccag acaatggctc
      721 cgaccgccag cagtaacagt ccggcgttgg cgagggaggc gaggatggtg cccttgcgca
      781 gcccgtagct gcggcgggac gtcggcaggg cgcgggccag aaccagggcc ccccaggcca
      841 tcagcaggcc cagcacgtcc gacaggttgt ggccggcgtc cgccatcagg gcgagcgacc
      901 cggtcagcag gccggcgagc gcctcgacgg cgacgaatcc gatgttgagc gccgcgccga
      961 tcgcgaaggc gcggccgaag tcggccggcg cgtggctgtg gccgtgcccg tgcccgtgcc
     1021 cgtggccgtg gtcatgcgaa tggccgtgat ggccgtgatc atcgtgcgcc atgggggtca
     1081 gagtctcact gccggcccgc cgcgtccacc agcgcctctg cccgacgctg ggccgcgctg
     1141 aggcgcccgg tccagtcggc ggccagggcc tcgagatcgt cggagcggct ggcgaagacg
     1201 gggccgtccc agaccatcgc cccccggccg aacggcagcg gcacgatggt ccggtcccag
     1261 ctgccgatcc gcaggcacgg ccggatcgcg agtccaagga agagcacggg cgcgccggtc
     1321 atcctggcca gggtcggggc gccgccctgc atcacctcgg ccgggccgcg cgggccatcc
     1381 ggcgtaatgg cgaccccgcc gccgctgcgg acccatttga ccatgtcgcg cagcgcctgc
     1441 tcgccatgct tgttcttggc cgcgtcggtg accttcttcg acgaaccgcg gatcgcggga
     1501 aagccgatct tctgaacggt cagcgcgatg aactcgccgt cggccgagcg ggaaatcagg
     1561 gcccgcatgt cctggcgatg cggggactgg ggccagctcg gtggggccag cggaatgacc
     1621 gcgtgccaga agcagagaat ggcgccgccc tcggtcgccc ataccgcctc gaccctggcc
     1681 tggtcctgcc ggctccagcg cacggtcgcg tagcacagcc tcagccaggc cgcgaacagg
     1741 ctggcgaggg tctgctgcac gaaggcggac cggaagaagg ccttcacgcg ggggtctcct
     1801 ccggcgctgt gtcgaggttc tgggcccggg ccagccgggc gtagaggccc cgcctgcgca
     1861 ccagggacgc atgggcgccc gattcgacga cgcggccgcg gtcgatgaca tagatgcgat
     1921 cggcgtgctt cacggtcgac agccggtgcg cgatgacaat ggtggtgcgg ccggccatca
     1981 gacggtcgag cgcctcctgg acctgcgcct cgctctcggt gtcgagggcg ctggtcgcct
     2041 cgtcgagcag caggatcggc gcgttcttga ggaaagccct ggcgatggcg atgcgctggc
     2101 gctggccgcc ggacaggcgg gccgccgcct ccccgaccac ggtgtcgtag ccatccggct
     2161 gctcgaggat gaagtcgtgg gcggcggccc gtcgggcggc ctcctcgatc tcggcgtccg
     2221 tcgcttcggg ccgggcgtag gcgatgttgg cgcggatcgt gtcgtcgaac aggaacggct
     2281 cctgggtgac gaaggcgatc cgggcgcgca gcgaggcgat ggtcacctcg cgcacgtccg
     2341 acccgtcgac ggtcacccgg ccgctggtgg cgtcatagaa gcgcgggatc agcgacagga
     2401 tggtgctctt gccgccaccc gatgggccga ccagcgcgac catctcgccg cggcgcgcct
     2461 cgatcgtcac gccatcgagg atcgcgccac cgtcgtcgta ggcgaaaccg acgtcctcca
     2521 gccgcacctc gccatcgccg gccaccaggg cgtgggccga taccgactcg cgcacctccg
     2581 gctcgacatc cagggccagg aacagtcggc gcgcggcggt catgccctcg gcgaagacgg
     2641 tctgcagatt ggccagctgc cgcagcgact ggccggccat gcccagcagg gcgaagaagg
     2701 ccatgaagtc gccggggttc atgttgtggc gccagccgac gtagacgaag atggccgccg
     2761 taacgaacac ggtgagcagt tcggtcacag gagcggactg ggcgcgggcg ttggccccct
     2821 tgatgatgaa cttctggcgc gcgtcgatca ccgccgcgac ccgcgcttcc tcgtccgctt
     2881 cccggttctc catcttgacg acgcgaacgc cgtcgaggct ctccatcagc gcggtcgaca
     2941 ggtcgctggt ggccgccatg gcgcccttgg cggccttctt ggagcgacgc gagaagcggc
     3001 gcatcaggcg gcccgcgaag ggcccgacca gggccacgcc ggcggccagc acggggtccc
     3061 ggtcgatcat ggccaccagg cagccaccga cgatcagcag attctgggtc cagttgacca
     3121 cgccgctcgt cgccgcctcg cggatcaggg cggcgtcgta gagcacctgg gacacgaagc
     3181 ttcccgaatg ggtcgcgcgc aggcgggcca ggtccgcgcg aaccatgcgg ccgaacagct
     3241 cctgctggat gtcgcccacc aggccatgtc cgatgacgtt gaccagcatc gcctggacga
     3301 tctgcgaagc cgcgcgaccc aggccatagg cgatgatggt cagggggatg atgacaatcg
     3361 ccccgggttt gggacttcga ccgagcaggt cgttcaccgc cggctcgatc atctgggcca
     3421 gcagcgtgct gaacaccgcc acgccgatgg cgcccagcaa cgccagggtg acgccccgcc
     3481 agcggcgccg gagataggtg cgccagacgc gggccgccag ctggcgatcg gtcatttcgg
     3541 ctggcgcggc ggcggtcatg gatgggggaa tcggccctga agacgtttgc gttcgacgcg
     3601 caggccacta cttaggacgc tcttgcaggg agcgccactt gtcgaagcgt ttcgaccttt
     3661 ccacgggctg gggccgcttc aaggcgtact ggcactcgat ctggggcgac catgccttcc
     3721 tgcgtgtcgg attctccaac gcccactgga tcgggcccga catggtccgg gccgcccagc
     3781 cctggcccta ccagctgaag tactggaagc gcgacggcat caggaccatc gtcaacctgc
     3841 gcggcggctt cgacacgggc ttctatgtgc tcgagcgcga ggcctgcgaa cggctgggcc
     3901 tgacgatggt cgacttcacc atcacctcgc gcgaggtccc ccgccgcgag accgtgctgg
     3961 gcgcgcgcga cctgttccag agcatcgagt atccggcgct gatgcactgc aagtccggcg
     4021 cggaccgggc cggcatcatg agcgtgttct accgccactt tcatctcggc gagccgatcc
     4081 gcgaggccat gaaggaactc ggcctgcgca ccctgcacgt caagcagggc aagaccggcg
     4141 tgctggacta tgtgttcgag cgctacctcg ccgaggccga gccgaagggg atcagcttca
     4201 tcgactgggt ccagagcgac gcctacgacc gcaagcagat caaggacgac ttcaaggcca
     4261 gctggtgggg aaccctgctg accgaccgcc tgctgcggcg ggaatagcgg gggacacgta
     4321 ccgcaggtac gtgtccccca atcgagctgc gggtttaggg ggcacgtacc tgcggtacat
     4381 gtcccctcga agtctcagtg ctggtcgtcg tcttcgttgg ggtcgagcag cttgtgggcg
     4441 tggatgatga aatagcgcgc gtgggcgttg tcgacggtcg cctgggcctt gcccttccag
     4501 gccttccagg cctcggcgta gttgggatag gcgccgacga actcgacctt gctcaggtcg
     4561 cggaactcga cgtcctggac gtccttcagt tcgccgccga tgacgatgtg cagcagttgt
     4621 ctgtccggca tgtgccgagg ccctttccgt agggaaaatc agttgggcag gtcgatatgc
     4681 cggacgcgct caaggatcaa cgggtgcaga gccggcgcgg cggagatcag gctgggcagg
     4741 cgggggctgg gctggttgta gcgatagccc cggcccttgt ggtcggtgca gaccgcgctg
     4801 gcctcggcgc agatcaggtc ggccgcggcc aggtcccatt cgctcttgcc gctcagcgcc
     4861 agggccgcgt cgaaccggcc gtcggcgacc aggcacatgc ggtaggcgat ggagttgcgc
     4921 gtctcgatgg tcatcggcgg ccagggctcg cgccaggcgg ggtgggcgaa catcttggcg
     4981 tcgccgagca tgcggcagtc ctcgacccga gcctgctcgc tggcgcgaat cgccgtgtcg
     5041 ttgaggaagg cgccgccgcc ggcggtggcg gtgaaggtct cgtccagctc cggcgcgaac
     5101 acgaccccgg ccgtcggccg gcccgcatcg accacggcga tcgacaccga ccaccagggc
     5161 cggttcttga gaaaggcggc cgtcccgtcg atcggatcga cgacgaacaa ccgccgccgg
     5221 tccagccggg ccgcatcgtc ggcggtctcc tccgacagcc agccatagtc gggccgcgcg
     5281 gcctccagcc tggccttgag cagctggtcg acggcgatat cggcgtcggt caccggcgag
     5341 ccgccggtct tggaccagat cttcaggccc tcgcccttca tgcgccgggc cagatcgccg
     5401 gcttcacgcg ctgtttcgac gatgagggcc aggtcgtcct tcatcggccg gcgatcggca
     5461 tggcgtcgac cagcaacgac ggggcgttgg ccgcgccgcg gacctccagg tccgagccag
     5521 gaaccagccg gccccagata tcgatcaggt tgccggccac ggtgatctcc gtcaccggat
     5581 aggcgatctg gcccttctcg aaccagaagc cggagcagcc caccgaccag tcgccggtgt
     5641 tggcgttgag cgaggggccg aacatcgagg tgatcagcag gccatcgccg gcgtcggcca
     5701 tcagcccggc cagatcgcgc tcgcccggct cgagcgtcag gttggtggtc gacacgcccg
     5761 gcggaccggc caggccgcgc gaggcgtggc ccgtggtctg catccccagc tgtttcgcgg
     5821 tggccgtgtt cagcagccag gtggtcagca ccccgtcctc gatgatcgcg gtgcgccggt
     5881 tggccacgcc ctcgtcgtcg aacggcgagg aacccaggcc gcgcacgcgg tgcggatcgt
     5941 cgacgatctg gacgccccgc gaaaacagcg gctgccccag ccggtccttg aggaagctga
     6001 cgccacgcgc cacggacggg cccgagatgg ccccgatcaa cggcccgatc agggagatgg
     6061 ccagccggtt ctcgaagatc accggggcgg tgatcgagtc gatcttgcgc gcgcccaggc
     6121 gctggacggc gcgcttgccg gcctcggcgc cgatggcggt cggcgagggc aggtcggccc
     6181 cgtggcggac ggtgcg
//
LOCUS       DAORNX010000136         6175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9430_length_6175_cov_6.977288, whole genome shotgun sequence.
ACCESSION   DAORNX010000136 DAORNX010000000
VERSION     DAORNX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9430_length_6175_cov_6.977288"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /gene="ispF"
                     /locus_tag="PLO65_07475"
     CDS             <1..658
                     /gene="ispF"
                     /locus_tag="PLO65_07475"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRD28123.1"
                     /translation="FRRDRLQAAYDAWTGAEPTDDAQVVEAAGGRVVVTPGDPRLMKL
                     TYPEDFAMAELLAGPGRAVRVGQGFDAHRWGPGEAVWLCGVRIDHDQTLVGHSDADAG
                     LHALTDALLGAIGEGDIGDHFPPTDPRWRGVSSDRFLDHAASLVAAKGGRIVNVDVTL
                     ICERPKIKPHRQAMRERLAAILDLPIDRVSVKATTTEGMGFTGRGEGLAAQAIAAVEM
                     "
     gene            complement(682..2931)
                     /locus_tag="PLO65_07480"
     CDS             complement(682..2931)
                     /locus_tag="PLO65_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRD28124.1"
                     /translation="MTDTIEARTTATTPTPPLFVVAVFSSAALVFMVQPMVAKLVLPL
                     LGGSPAVWNTSMAFFQIALLVGYGYAHVLQRVPQLRRQIGVHIAALVIAGLSLFPLSI
                     STLAGEPSSNHPALWLLGVLTLSIGLPFAILSATAPLVQAWHARVFRGEAGKEPWSLY
                     AASNLGSLLALLAYPVVMEPLMTLQAQRWSWSGGYWAFVLILTALAVLVWKRATLAPG
                     DIEAAPARLTPAPTWLRRATWVALAAIPSSLMLGVTAHLATDVASAPFLWVAPLALYL
                     VTFIIAFQTRPLISTGGALIFQGAAVAACAAVLQLFAAGAIELLVFVHLSCFFLTALV
                     CHQALVARRPDPEHLTEFYLCMSLGGVVGGSFNAFVAPLIFNTVLEYPLVLALSCLAR
                     PWGRGRLTYWQAAVLGTGVLGAILAVAAVPHVGQQDITVKGSLAAATVAVFLLRGRAL
                     YMFAMIVVLLVAADRVGDRADVIRTDRSFFGVLRQTRTTIPGLGGEVRMLAHGTTLHG
                     AQAQDPRYRCRPLVYYAPETPIGQVFTAAQAMQPALRIGAVGLGTGSVSAYVRPSDHL
                     TFFEIDPLVIRISRDSGAFTYIRDCATGAVDFVLGDARLTLARQPADSFDILLIDAFS
                     SDSVPAHLLTVEAVRGYLARLKPDGVLILHLSNRNLELRAPAMAVAKAAGGYALLQRH
                     NKDWQTPNMWESSEDALIIGRSAAALAPYEADERWQLSDPTLARPWTDDYTNLVGALW
                     GRLKERFER"
     gene            3014..3502
                     /locus_tag="PLO65_07485"
     CDS             3014..3502
                     /locus_tag="PLO65_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRD28125.1"
                     /translation="MFPLEVETLARLLIDEVRGRHLRVTTAESCTGGLVAAAICSIPG
                     ASDIFERGFVTYTNRAKQEMLGVPGDLIADFGEVSEPVARMMAEGALSHSHAHIAVAI
                     TGVAGPGGGSPMKPVGTVHLATARSTAGIRHRHELFEQDTRLGVQMAAVAVALQMMRD
                     AI"
     gene            complement(3618..4067)
                     /locus_tag="PLO65_07490"
     CDS             complement(3618..4067)
                     /locus_tag="PLO65_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD28126.1"
                     /translation="MKHRLTRILPYTPDQLFQLVGDVERYPEFVPWITALRVWNRQDP
                     GGGVTLLDAEAAVGFSFLRERFSTRVRRDAAARTIDVSLLSGPFRRLQNRWRFVEHPE
                     GVCVEFEIDFEFKLGFLDRLLHANFDHAVARLIQCFDDRARVLYGTR"
     gene            complement(4087..5046)
                     /gene="lipA"
                     /locus_tag="PLO65_07495"
     CDS             complement(4087..5046)
                     /gene="lipA"
                     /locus_tag="PLO65_07495"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRD28127.1"
                     /translation="MATVIDTLQARPRHPEKQNRPETPLLRKPEWLRVRAPGSAGYND
                     TRDIVRSHGLTTVCEEAACPNIGECWSQKHATMMIMGEICTRACSFCNVITGKPNALD
                     PTEPGRVAEAVATMGLKHVVITSVDRDDLDDGGAAHFAAVVHAIRAAAPDTTIEILTP
                     DFLRKDGAAEVVIDSAPDVFNHNLETVPRLYLSIRPGARYYNSLRLLERVKDRDPRRF
                     TKSGLMVGLGESKEEVMQVMDDMRSAGVDFLTIGQYLQPTRRHAAVDRFVHPDEFAAY
                     EAIARAKGFLMVSASPLTRSSHHAGEDFARLKAAREALDSAAR"
     gene            5274..>6175
                     /locus_tag="PLO65_07500"
     CDS             5274..>6175
                     /locus_tag="PLO65_07500"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28128.1"
                     /translation="MLRRATLLCLLLLAACGGGDGGAPFVESRIPPALGPRFWAPEGW
                     AWGLLKIGDAPAQRYGVSSTPVTPQAQILILTGYGESAEAWFETARDLNARGYSVWVL
                     ERAGQGGSERYVLPRDLGHAPQFRDDIAAVRTLSRMMAAGAPGRPLIVLGHSVGGLVA
                     VAAIEQGAPAEGLILSAPAFADDDLIDRGKAGLVKAGLGRLPAAWNAGWSRSGPDGRA
                     SGLTADPLRGGVQMAWQTANPDLRMGGPSLGWLASFRAASRGIEGDLAALETPTLMLA
                     GGLDRRVDAAAQIRVCRAMPHCRR"
BASE COUNT          947 a         2146 c         2228 g          854 t
ORIGIN      
        1 cttccgccgg gaccgcctgc aggccgccta tgacgcctgg accggcgccg agccgaccga
       61 cgacgcccag gtcgtcgagg ccgccggcgg ccgcgtggtc gtcacgcccg gcgatccgcg
      121 cctgatgaag ctgacctatc cggaggattt cgccatggcc gagctactgg ccggccccgg
      181 tcgcgccgtg cgcgtcggcc agggcttcga cgcccatcgc tgggggcccg gcgaggcggt
      241 ctggctctgc ggggtgcgga tcgaccatga ccagaccctc gtcgggcatt cggatgccga
      301 cgcggggctg cacgccctga ccgacgccct ccttggcgcc atcggcgagg gcgacatcgg
      361 cgaccacttc ccgccgaccg atccgcggtg gcgaggcgtg tcgagcgacc ggttcctcga
      421 ccatgcggca tcgctcgtgg cggccaaggg cggccggatc gtcaatgtcg atgtcacgct
      481 gatctgcgaa cggccgaaga tcaagccgca ccgccaggcc atgcgcgagc gactggcggc
      541 gatcctcgac cttccgatcg accgggtcag cgtcaaggcc acgaccaccg agggcatggg
      601 gttcacgggc cgtggcgagg ggctggccgc ccaggcgatc gcggcggtcg agatgtagca
      661 gggccaggcg gggccccggc cctatcgctc gaaccgctcc ttcagccgtc cccacagcgc
      721 cccgaccagg ttggtgtagt catcggtcca gggccgggcc agggtcggat cggacagctg
      781 ccagcgctcg tcggcctcgt agggggcgag ggccgcggcc gaccggccaa tgatcagggc
      841 gtcctccgac gattcccaca tgttcggcgt ctgccagtcc ttgttgtgcc gttgcagcag
      901 ggcgtagccg ccggccgcct tcgccacggc catggccggc gcgcggagct cgaggttgcg
      961 gttcgacagg tgcaggatca gcacgccgtc cggcttgagc cgggccaggt agccacgcac
     1021 cgcctcgacg gtcagcagat gggccggcac ggagtccgac gagaaggcgt cgatcagcag
     1081 aatgtcgaaa ctgtcggccg gctggcgggc gagggtcagc cgcgcgtcgc ccagcacgaa
     1141 gtcgacggcg ccggtggcgc agtcacgaat gtaggtgaag gcgccggagt cccgcgagat
     1201 gcggatcacc agcggatcga tctcgaagaa ggtcaggtgg tcgctcggcc gcacataggc
     1261 cgagaccgag ccggtgccca ggccgacggc gccgatgcgc agggccggct gcatcgcctg
     1321 ggcggcggtg aacacctggc cgatcggcgt ctccggcgcg tagtagacca gcggccggca
     1381 gcggtagcgt gggtcctgcg cctgggcccc gtgcagcgtc gtgccgtggg ccagcatgcg
     1441 gacctcgcca ccgaggcctg gaattgtggt gcgtgtctgg cgcagcacac cgaagaagct
     1501 gcgatcggtg cggatcacgt cggcccggtc gccgacgcgg tcagccgcga ccagcagcac
     1561 gacgatcatc gcgaacatgt agagagcccg cccgcgcagc aggaacaccg cgaccgtcgc
     1621 cgcagccaga gagcccttca ccgtgatgtc ctgctgcccg acatggggga cggcggcgac
     1681 cgccaggatc gcgcccagca cgccggtccc cagcacggcc gcctgccagt aggtcagccg
     1741 gccccggccc cagggcctgg ccaggcagga cagggccagc accagcggat actcgagcac
     1801 agtgttgaag atcagcggcg cgacgaaggc gttgaacgag ccgccgacca ccccgcccag
     1861 cgacatgcac agatagaact cggtcagatg ttcggggtcg ggccgccgcg cgaccagcgc
     1921 ctgatggcag accagggcgg tcaggaagaa gcagctcagg tggacgaaca ccagcagctc
     1981 gatcgcgccg gccgcgaaca gctgcagcac cgcggcgcag gcggccaccg ccgcgccctg
     2041 gaagatcagg gcgccgcccg tcgagatcag cggccgcgtc tggaaggcga tgatgaatgt
     2101 gacgagatag agcgccagcg gcgccaccca gaggaacgga gcggaggcga catcggtggc
     2161 cagatgggcg gtgacgccca gcatcaggct tgacggaatg gccgccagcg ccacccaggt
     2221 cgcgcgccgc agccaggtcg gcgcgggtgt cagccgcgcg ggggccgcct cgatgtcgcc
     2281 gggcgccagc gtggcccgtt tccacaccag gaccgccagc gccgtcagga tcagcacgaa
     2341 cgcccagtag ccgccgctcc agctccagcg ctgggcctgc agggtcatca gcggctccat
     2401 caccaccgga taggccagca gggccagcag gctgccgagg ttgctggcgg cgtagaggct
     2461 ccagggttcc ttgccggcct cgccgcggaa cacgcgggcg tgccaggcct gcaccagcgg
     2521 cgcggtggcc gacaggatgg cgaacggcag gccgatcgac agcgtcagca cgcccagcag
     2581 ccagagcgcc ggatggttcg acgatggctc gccggccagg gtcgagatgc tcagcggaaa
     2641 caggctcagg cccgcgatca ccagggcggc gatatgcacg ccgatctgcc gtcgcagctg
     2701 cggaacgcgt tgcaggacat gggcgtagcc gtagccgacc aacagggcga tctggaagaa
     2761 ggccatgctg gtgttccaca ccgccggcga gccgcccagc agcggcaaca ccagtttggc
     2821 caccatcggc tggaccatga acaccagcgc cgcgctgctg aagacggcga cgacgaacag
     2881 cggcggcgtc ggcgtcgtcg cggtcgtccg ggcctcgatc gtgtctgtca tggctcgaat
     2941 cggcttgtca gtcggaggtg ggcgatgttg ggctgcgtcc cgtcgttttg gccagccccc
     3001 ggagaccgct gttatgttcc ccctggaagt ggagaccctc gcccggctgc tgatcgacga
     3061 ggtgcgcggg cgtcacctgc gggtcaccac ggctgaaagc tgcacgggcg gcctggtcgc
     3121 cgccgcgatc tgctcgatcc ccggggcgtc ggacatcttc gagcgcggct tcgtgaccta
     3181 caccaaccgc gccaagcagg agatgctggg cgtccccggc gacctgatcg ccgacttcgg
     3241 cgaggtctcc gagccggtgg cgcggatgat ggccgagggc gccctctccc actcccacgc
     3301 ccatatcgcg gtggccatca ccggcgtggc cggcccgggc ggcggttcac cgatgaagcc
     3361 ggtcggcacg gtgcatctgg ccacggcccg cagcacggcc gggatccgtc accggcacga
     3421 gctgttcgag caggacaccc ggctgggcgt gcagatggcg gccgtggccg tggccctgca
     3481 gatgatgcgg gacgcgatct gaatgcgtcc tgcggcatgc cgcttcgcgg ctgcccagga
     3541 tgacgtgcag acgtgggcgg caatttccac gtcatggtga gcagcgccga aggcgcgtgt
     3601 cgaaccaggc gaacgggcta gcgagtcccg tacagcaccc gcgcccggtc atcgaagcac
     3661 tggatcagcc gggcgacggc atggtcgaag ttggcgtgca gcagccggtc gagaaagccc
     3721 agcttgaact cgaagtcgat ctcgaactcg acgcagacgc cttccggatg ctcgacgaac
     3781 cgccagcggt tctgcagccg ccggaacgga ccgctgagca ggctgacatc gatggtccgg
     3841 gcggccgcgt cgcgacgcac gcgggtcgag aatcgctccc tgaggaacga gaagcccacc
     3901 gccgcctcgg cgtccagcag ggtcacaccg ccgccggggt cctggcggtt ccatacgcgc
     3961 agggcggtga tccagggcac gaactccgga taccgctcca cgtcgcccac cagctggaac
     4021 agctggtccg gcgtgtaggg caggatgcgc gtgagcctgt gcttcaaaag gtcccccgac
     4081 ccccggctat cgggccgcgc tgtccagcgc ttccctggcc gccttgagcc gggcgaaatc
     4141 ctcgccggcg tggtgcgagg agcgggtcag ggggctggcc gagaccatca ggaagccctt
     4201 ggcccgggcg atcgcctcat aggccgcgaa ctcgtcggga tggacgaacc gatcgacggc
     4261 cgcatggcgg cgcgtcggct gcaggtactg gccgatggtc aggaaatcga cgccggcgga
     4321 gcgcatgtcg tccatcacct gcatgacctc ctccttgctc tcgcccagcc cgaccatcag
     4381 gcccgacttg gtgaagcggc gcgggtcgcg gtccttgacc cgctccagca ggcgcaggga
     4441 attgtagtag cgggcgcccg gtcggatcga caggtagagc cggggcacgg tctcgaggtt
     4501 gtggttgaac acgtccgggg cggagtcgat gaccacctcg gccgcgccgt ccttgcgcag
     4561 gaagtcgggg gtcaggatct cgatggtcgt gtccggcgcc gcggcgcgga tggcgtgaac
     4621 cacggcggcg aagtgagccg cgccgccgtc gtccaggtcg tcgcgatcga ccgaggtgat
     4681 gaccacatgc ttgagcccca tggtcgccac ggcctcggcg acccgccccg gctcggtggg
     4741 atccagggca ttgggcttgc cggtgatcac attgcagaac gagcaggccc gcgtgcagat
     4801 ctcgcccatg atcatcatgg tcgcgtgctt ctggctccag cactcgccga tgttcgggca
     4861 ggccgcctct tcgcagaccg tggtcaggcc gtgcgaacgg acgatgtcgc gcgtgtcgtt
     4921 gtaaccggcc gagccgggcg cgcgcacgcg cagccactcg ggcttgcgca gcagcggagt
     4981 ctcgggccgg ttttgcttct cgggatggcg cggacgcgcc tgcagggtgt cgataaccgt
     5041 ggccatggcg cctaaatcgg ctttccgggc cgccggatca agctcggagg gccggcaaaa
     5101 cctcgccatt gcccgattgt ccccgccagg gcgggacacg agccgaacgg ggctaccgcc
     5161 cgacctcgcc gactctgaac accgctcgcg cgggatgcac ggaacccgga acgacttccg
     5221 cctcagggga agggccttgc ggtggctcgt cgatccgccc gacagtcgct ccgatgctgc
     5281 gtcgcgccac cctcctctgc cttcttctgc tcgcggcctg cggcggcggg gacggtggcg
     5341 cgccgttcgt cgagagccgc attccgccgg ccctcggccc gcggttctgg gcgccggagg
     5401 gctgggcctg ggggctgctg aagatcggcg acgccccggc gcaacgctac ggcgtgtcct
     5461 ccacgccggt cacgccccag gcgcagatcc tgattctgac cggctacggc gagtcggcgg
     5521 aggcctggtt cgagacggcc cgcgacctga acgcccgcgg ctacagcgtc tgggtcctgg
     5581 agcgggccgg ccagggcggg tcggagcgtt atgtcctccc ccgggatctg ggccacgcgc
     5641 cgcagttccg cgacgacatc gcggcggtca ggaccctgtc gcggatgatg gccgccggcg
     5701 cgcccggccg gccgctgatc gtcctcggcc attccgtcgg cggactggtg gccgtcgccg
     5761 ccatcgaaca gggcgcgccg gccgaagggc tgatcctgtc ggctcccgcc ttcgccgacg
     5821 acgatctcat cgaccgcggc aaggccggtc tggtcaaggc cggccttggc cgcctgcccg
     5881 cggcctggaa cgccggctgg agccgttcgg gacccgatgg tcgggccagc ggcctgaccg
     5941 ccgacccgct gcgaggcggg gtgcagatgg cctggcagac ggccaatccg gacctgcgga
     6001 tgggtggccc cagcctcggc tggctggcca gcttccgcgc cgcctcccgg ggcatcgagg
     6061 gggacctggc ggcgctggaa acccccacgc tgatgctcgc cggtggtctc gaccggcggg
     6121 tggacgccgc ggcccagatc cgtgtctgcc gggccatgcc ccactgccgc cgcta
//
LOCUS       DAORNX010000137         6168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9444_length_6168_cov_7.099297, whole genome shotgun sequence.
ACCESSION   DAORNX010000137 DAORNX010000000
VERSION     DAORNX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_6168_cov_7.099297"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLO65_07505"
     CDS             <1..598
                     /locus_tag="PLO65_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD28129.1"
                     /translation="GAPEAAPVEALELPAMPVGAQNPYTIGASIARHMPEGAIISDDA
                     VTAGLPIFQATKTARPHDWLMLTGGAIGQGIPLAIGAAVACPDRKVLSLNGDGAAMYT
                     VQGLWTIAREQLDVTVVVFANHAYRILNIEMGRTGAGNPGPAAKKLLDLGDPRIDWVH
                     VASGLGLPAERCSTVEAFDEAFERAMNQTGPRFIEAAL"
     gene            complement(749..2092)
                     /locus_tag="PLO65_07510"
     CDS             complement(749..2092)
                     /locus_tag="PLO65_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28130.1"
                     /translation="MKIIEIGLAAALALFVAAPASTVMAAKKPAAAEGGVDAKSREKG
                     MAEAPPLVAAAGLSCTLTDARWIGADAKTKQGFYEVACNPGLGGVLVASKDATTPPSF
                     YPCLETGKPGPDGKPGPLTCKLPGNADPVQALAPFVAKSGVACTIDKARSIGASSKNT
                     FFEVACQGGYGYIMVASFPADANKDVKANTCLAYEPGGNLFCELTSRETQLAMVDQLA
                     AASGKNCTVTDKRYILSAVNGSMYFEVACSDGKGYVLERAANGSLARAIDCANAPGGA
                     ECTLTDAREAKTEQAGLYSRLAAKAGFPCKVEKYALFPSRGKPEEVVELKCSDRPDGG
                     VAVFDANGGGKVYNCIVAELQGYACSFTPKAASMVKVSNDLKALGKGSCVVSDARVIG
                     KTDTGDGFLEVACADGLPGWVLNYATGSDSPKEVLSCKQASGIGGGCKLPTNKSS"
     gene            2233..3303
                     /locus_tag="PLO65_07515"
     CDS             2233..3303
                     /locus_tag="PLO65_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD28131.1"
                     /translation="MRDGLEDTATEVRRPKLDYPFESGPTPGTGEAFDVAPGVKWLRM
                     PLGGPLQFINVWAIEDGDGWCVVDTGMQTRDTSQAWRTAFKDALGGRPVTRVIVTHLH
                     PDHIGLAGWMTRKFQCRLWMTRLEYLQCRMLVADTGREAPEDGMRFYKAAGWDEDALE
                     NYRARFGGFGKAIYQLPDSYRRLNDGEEFEIGGRTWRIVTGNGHSPDHACLYCPEARL
                     LISGDQVLPRISSNVSVFPTEPDADPLSDWMTSCAKVKREVPDEVLVLPAHNDPFHGL
                     HARLDHLVAGHERSLSRLEKALAEPKRAIDVFGSLFARPIGPELVGMATGESLAHINC
                     LIERGKAVAEADADGVIWYRAV"
     gene            3391..3822
                     /locus_tag="PLO65_07520"
     CDS             3391..3822
                     /locus_tag="PLO65_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD28132.1"
                     /translation="MTERPVFYALLKAAQKRVERWVEAEAAQIEGVTSAQAGLVLFLG
                     RSDGAAIGDAAEALDVAPSAMTGLVDRMTRAGYVERRPDPTDGRGQRLYLTPQGWSAR
                     ERAVALLDDLNHRMSEGLSEEDATAVARWLDSVATKFSRED"
     gene            3827..4795
                     /locus_tag="PLO65_07525"
     CDS             3827..4795
                     /locus_tag="PLO65_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28133.1"
                     /translation="MTDRITSPFGAFTPAREVVSGVDLSGKTAIVTGAATGIGIETAR
                     ALAEAGADVVIAARKPDLAAAAIADIDRTAPGRARFEMLDLSELASIRAFAARWSGKP
                     LHILINNAAVMACPLDRTHDGFEMQIGTNHFGHFLLGVLLAPALEAGAQAAGRPSRLV
                     ALSSIGHRRSDINWEDVNYVTRPYDKWESYGQAKTANSLFAVGFHKRFKDRGITANAV
                     MPGGIMTPLQRHMQHEEMVAMGWIDPETGKIRDGFKTPEQGASTSVWAAVGPELEGVG
                     GLYLENCAQALPWSKESPFTGVMPHALNPESAERLWALSVETVGAG"
     gene            4950..5807
                     /locus_tag="PLO65_07530"
     CDS             4950..5807
                     /locus_tag="PLO65_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28134.1"
                     /translation="MSLAGKTLFITGASRGIGLAIGLRAARDGANVAIAAKTAEPHPK
                     LPGTIYTAAEEIEKAGGRALPLIVDVREEASVYDAVERTVAAFGGIDICVNNASAISL
                     TPTEATDMKRYDLMHQINARGTFLTSRACIPHLKRAANPHVLALSPPLDLSPHWFGRH
                     VAYTVAKYNMSMFMLGMADEYRDDGIAFNGLWPRTGIATAAIQFALAGDEGMRHCRTP
                     EIMADAAHAIFCKPARDFTGQFLIDDTFLYGEGVRDFEKYRVDPTKPLMPDFFVPEAN
                     PAPPGVKIG"
     gene            5804..>6168
                     /locus_tag="PLO65_07535"
     CDS             5804..>6168
                     /locus_tag="PLO65_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28135.1"
                     /translation="MRLIVRPAHPGDAPVILGFIEALAEYEKLRHEADLTLWNVEVSF
                     FGVAPKVFCDIAELDGRPVGLAVWFYSYSTFRGRHGIWLEDLFVEEGARGHGAGRALL
                     AGLARRCRDEDLPRLDWSVL"
BASE COUNT          975 a         2094 c         2147 g          952 t
ORIGIN      
        1 gggggctccc gaggcggcgc cggtcgaagc gctcgagctg ccagccatgc cggtgggcgc
       61 ccagaacccc tacaccatcg gcgcctcgat cgcccggcac atgcccgagg gcgccatcat
      121 cagcgacgac gcggtgaccg ccggcctgcc gatcttccag gccacgaaga ccgcgcggcc
      181 gcacgactgg ctgatgctga ccggcggggc catcggccag ggcatcccgc tggccatcgg
      241 cgcggccgtg gcctgccccg accgcaaggt gctgagcctg aacggcgacg gcgcggccat
      301 gtacacggtg cagggcctgt ggacgatcgc ccgcgaacag ctggacgtga cggtcgtggt
      361 gttcgccaac cacgcctacc gcatcctcaa catcgagatg ggccgcaccg gcgccggcaa
      421 ccccggcccg gcggcgaaga agctgctcga cctgggcgat ccgcgcatcg actgggtcca
      481 tgtcgccagc ggcctgggcc tgccggccga gcgctgctcg acggtcgagg cgtttgacga
      541 ggcgttcgag cgggcgatga accagacggg cccgcggttc atcgaggcgg cgctctgacg
      601 aagcgacggg gacatgtacc gccttcggta cgtgtcccct gccgctcaag ctgatgggga
      661 cacgcactga agtgcatatc cccccgcaaa cgaaaaaggc ccgccggatc gctccggcgg
      721 gccttctggt atcagtcgcg agggccgatc agctggactt gttggtcggc agcttgcagc
      781 cgccgccgat gccggaagcc tgcttgcagc tgaggacttc cttggggctg tcggaacccg
      841 tggcgtaatt cagcacccag ccgggcaggc cgtcggcgca ggccacctcg aggaagccgt
      901 cgccggtgtc ggtcttgccg atcacccggg cgtcggacac cacgcaggag cccttgccga
      961 gcgccttcag gtcgttgctg accttgacca tcgaggcggc cttgggcgtg aacgagcagg
     1021 cgtagccctg cagttcggcg acgatgcagt tgtagacctt gccgccgccg ttcgcgtcga
     1081 acaccgccac gccgccgtcg gggcggtccg agcacttcag ctcgaccact tcctcgggct
     1141 taccgcgcga ggggaacagg gcgtacttct cgaccttgca ggggaagccg gccttggcgg
     1201 ccaggcggct gtagaggccg gcctgttcgg tcttggcctc gcgggcgtcg gtcagggtgc
     1261 attcggcgcc gccgggggcg ttggcgcagt cgatggcgcg ggccagcgaa ccgttggcgg
     1321 cgcgttcaag gacgtagccc ttgccgtcgg agcaggcgac ctcgaaatac atcgagccgt
     1381 tgacggccga gaggatgtag cgcttgtcgg tgacggtgca gttcttgccc gaggcggcgg
     1441 ccagctggtc gaccatggcc agctgggtct cgcggctggt cagttcgcag aacaggtttc
     1501 cgcccggctc gtaggccaga caggtgttgg ccttgacgtc cttgttggcg tcggccggga
     1561 aggaggccac cataatgtag ccatagccgc cctggcaggc gacctcgaag aaggtgttct
     1621 tgctcgaggc tccgatcgag cgagccttgt cgatggtgca ggccacgccg gacttggcga
     1681 cgaacggcgc cagggcctgg accggatcgg cgttgccggg aagcttgcag gtcagcgggc
     1741 cgggcttgcc gtcaggaccg ggcttgccgg tctcgaggca ggggtagaac gacggcgggg
     1801 tcgtggcgtc cttgctcgcg acgagcacgc cgcccaggcc gggattgcag gcgacctcgt
     1861 agaagccctg cttggtcttg gcgtccgcgc cgatccagcg ggcgtcggtc agcgtgcagc
     1921 tcaggcccgc ggccgcgacc aggggcggcg cctcggccat gcccttctcg cgggatttcg
     1981 cgtcgacccc gccctcggcg gcggccggct tcttggccgc catgaccgtg ctggcgggcg
     2041 ccgccacgaa caaggccaga gcggccgcca ggccgatctc gatgatcttc atttcatgca
     2101 ctcctggcag aatcctcgcg cggacttaaa cgcgcgcttt tttcgagggt caagatcgcg
     2161 caaccggtcg gcggaattgc ggcgcggggc tgaaccgccg atgatggccg cgttcatcag
     2221 gagtgcagca agttgcggga cggattggaa gatacggcga ccgaggtccg gcgaccgaaa
     2281 ctggactacc cgttcgagtc cgggccgacc ccgggaaccg gcgaggcgtt cgatgtcgct
     2341 ccgggcgtca agtggctgcg catgccgctc ggcgggccgc tgcagttcat caacgtctgg
     2401 gccattgagg acggcgacgg ctggtgcgtc gtcgataccg gcatgcagac ccgcgacacg
     2461 tcccaggcct ggcggacggc gttcaaggac gccctgggcg ggcggccagt cacgcgcgtg
     2521 atcgtcaccc atctgcaccc cgatcacatc ggccttgccg gctggatgac gcgcaagttt
     2581 cagtgccggc tgtggatgac gcggcttgaa tatctgcagt gccgaatgct cgtcgccgac
     2641 accggccgcg aggcgcccga ggatggcatg cgcttctaca aggcggccgg ctgggacgag
     2701 gatgcgctgg aaaactaccg cgcccgtttc ggcggtttcg gcaaggccat ctaccagctg
     2761 cccgacagct atcgccggct caatgacggc gaggaattcg agatcggcgg ccggacctgg
     2821 cggatcgtca ccggcaatgg tcattcgccc gaccacgcct gcctctactg tcccgaggcc
     2881 aggctgctga tttcgggcga ccaggtgctg ccgcgcatct cctccaatgt gtcggtgttt
     2941 ccgaccgaac ccgacgccga tccgctcagc gactggatga ccagctgcgc caaggtcaaa
     3001 cgcgaggtgc ccgacgaggt gctggtgctg ccggcccaca acgatccgtt ccacggcctc
     3061 catgcccggc tcgaccacct ggtcgccggg catgaacggt cgttgagccg gctggaaaag
     3121 gcgctggccg agccgaagcg ggccatcgac gtgttcggat cgctgttcgc ccgcccgatc
     3181 ggccccgaac tggtcggcat ggccaccggc gagagcctgg cccacatcaa ctgcctgatc
     3241 gagcgcggca aggccgtcgc cgaggccgac gccgacgggg tgatctggta ccgggcggtg
     3301 tgaggaccct tcctctccgc gaagcgggga ggatcatcta tttcacatgt gaacttgatg
     3361 tttcgcatgt gaagcatata ttggcgttcg atgaccgagc gccctgtctt ctacgccctg
     3421 ctgaaagccg cccagaagcg cgtcgagcgc tgggtggagg cagaggccgc gcagatcgaa
     3481 ggggtgacct cggcccaggc cgggctggtg ctgttccttg ggcggtcgga tggcgcggcc
     3541 atcggcgacg ccgccgaggc gctcgacgtg gccccttcgg cgatgaccgg gctggtcgat
     3601 cgcatgaccc gcgccggcta tgtcgagcga cggcccgacc cgaccgacgg ccgcggccag
     3661 cgcctgtatc tgactccgca gggctggtcc gcgcgcgagc gcgccgtggc cctgctcgat
     3721 gatctcaacc accgcatgag cgaaggtctg tcagaggagg acgccactgc cgtcgcccgc
     3781 tggctggact ctgtcgccac gaaattctcc agggaggact gaccccatga ccgatcgcat
     3841 cacctcgccc ttcggggcct tcacgccggc gcgcgaggtc gtctccggcg tcgacctgtc
     3901 cggcaagacc gcgatcgtca ccggcgcggc caccggcatc ggcatcgaga ccgcccgggc
     3961 cctggccgag gccggcgccg atgtggtcat cgccgcgcgc aagcccgatc tggccgccgc
     4021 cgccatcgcc gacatcgaca ggaccgcgcc cggaagagcc cggttcgaga tgctcgacct
     4081 gtctgaactg gcctcgatcc gcgccttcgc cgcgcgctgg agcggcaagc cgctgcatat
     4141 cctgatcaac aacgccgccg tcatggcctg cccgctggac cgcacccacg acgggttcga
     4201 gatgcagatc ggcaccaacc acttcggcca cttcctgctc ggcgtcctgc tggcgccggc
     4261 gctggaggcc ggggcgcagg cggcgggcag gccgtcgcgg ctcgtcgccc tgtcgtcgat
     4321 cggccaccgt cgctcggaca tcaactggga ggacgtcaac tacgtcaccc gcccctatga
     4381 caaatgggag agctacggcc aggccaagac cgccaacagc ctgttcgcgg tcggcttcca
     4441 caagcgcttc aaggatcgcg ggatcaccgc caacgcggtc atgcccggcg gcatcatgac
     4501 cccgctgcag cggcacatgc agcacgagga gatggtcgcc atgggctgga tcgacccgga
     4561 gacgggcaag atccgcgacg gcttcaagac cccggagcag ggcgcctcga ccagcgtctg
     4621 ggcggcggtc gggccggagc tggagggcgt cggcgggctg tacctggaaa actgcgccca
     4681 ggccttgccg tggagcaagg agtcgccgtt caccggcgtc atgccccatg ccctgaaccc
     4741 ggaaagcgcc gagcggctgt gggcgctgtc ggtggagacc gtcggcgcgg gttgaggctg
     4801 cgtccttcaa gacgcgatcc cgagggatcg ctcctcagga tgacgtatat tgtcgaggag
     4861 cgaggcctca gccgagcgtc tcgaaccgcg caccctccac acgatggagg gggagggata
     4921 ggatgtcgtc gaacccttga ggagcgacca tgagtctggc cggaaagacc ctgttcatca
     4981 ccggcgccag ccgcggcatc ggcctggcca tcggactgcg ggcggcgcgg gacggggcca
     5041 atgtggccat cgcggccaag accgccgagc cgcaccccaa gctgccgggc accatctaca
     5101 cggccgccga ggagatcgag aaggccggcg gcagggcgct gccgctgatc gtcgacgtgc
     5161 gcgaggaggc cagcgtctat gacgcggtgg agcgcacggt ggcggcgttc ggcggcatcg
     5221 acatctgcgt caacaacgcc tcggccatca gcctgacgcc gaccgaggcg accgatatga
     5281 agcgctacga cctgatgcac cagatcaacg cgcgcgggac cttcctgacg tccagggcct
     5341 gcatcccgca cctgaagcgg gcggccaatc cgcacgtgct ggcgctgtcg ccgccgctgg
     5401 acctgtcgcc gcactggttc ggccggcacg tggcctacac cgtggccaag tacaacatgt
     5461 cgatgttcat gctcggcatg gccgacgagt accgggacga cggcatcgcc ttcaacggcc
     5521 tgtggccgcg cacgggcatc gccacggccg cgatccagtt cgccctcgcc ggcgacgagg
     5581 gcatgcggca ctgccgcacg ccggagatca tggccgacgc ggcccacgcc atcttctgca
     5641 agccagcgcg cgacttcacc ggccagttcc tgatcgacga caccttcctc tatggagagg
     5701 gcgtgcgcga cttcgagaag taccgcgtcg atccgacgaa gccgctaatg cccgacttct
     5761 tcgtgccgga agccaatccg gccccgcccg gagtaaagat cggatgaggc tgatcgtccg
     5821 cccggcccac cccggcgacg cgccggtcat cctcggtttc atcgaggccc tggccgagta
     5881 cgaaaagctg cgccacgagg ccgacctgac gctgtggaac gtggaggtgt cgttcttcgg
     5941 cgtcgcgccg aaggtgttct gcgacatcgc cgagctggac ggaaggccgg tgggactggc
     6001 cgtctggttc tacagctatt cgacctttcg cggccgccac gggatctggc tcgaggacct
     6061 gttcgtcgag gagggggcgc ggggccacgg cgccggccgg gcgctgctgg ccgggctcgc
     6121 gcggcgctgc cgggacgagg acctgccgcg gctcgactgg tcggtgct
//
LOCUS       DAORNX010000138         6136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9515_length_6136_cov_7.647098, whole genome shotgun sequence.
ACCESSION   DAORNX010000138 DAORNX010000000
VERSION     DAORNX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9515_length_6136_cov_7.647098"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLO65_07540"
     CDS             <1..230
                     /locus_tag="PLO65_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28136.1"
                     /translation="IRRAIASDRPELLALACAAIGVDRGAFPTILEAVRDLNQGRPGG
                     GAEAGRRATGAFGPFDADIAGVAFRKAVSTV"
     gene            266..1045
                     /locus_tag="PLO65_07545"
     CDS             266..1045
                     /locus_tag="PLO65_07545"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRD28137.1"
                     /translation="MFNSKPHTSRVTFLASDRPEAQEARERLIARYGDAGDSAQVIVA
                     LGGDGFMLETLHGALADGKPIYGMNRGSVGFLMNEYSEDGLLERISAAERAVIHPLAM
                     VAIDSKRKQHRALAINEVSLLRQTRQTAKLRISIDGKVRMGELVCDGALVSTPAGSTA
                     YNLSAHGPIIPIDARVMALTPISAFRPRRWRGALLSHTARVTFEVLESDKRPVSAVAD
                     NVEVRDVLEVHIAEDRSVSLAMLFDAGRSLEERVLAEQFSA"
     gene            1144..1626
                     /locus_tag="PLO65_07550"
     CDS             1144..1626
                     /locus_tag="PLO65_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRD28138.1"
                     /translation="MSDNPTVQVIQAPNTLRLKVGGRLGGIDPAAIAKAEAALKSLSG
                     NFSEWLNDELVKLEAARQRIRAEGLNTETAEGLYLRAHDLKGLGATYEFPLVTRIAGS
                     LCKLIDDPDTRLDAPMFLVDAHIDGIKASVRGNIRSEDHPVGRKLVEELEDRVAKLAA
                     "
     gene            complement(1623..2756)
                     /locus_tag="PLO65_07555"
     CDS             complement(1623..2756)
                     /locus_tag="PLO65_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRD28139.1"
                     /translation="MMPPTYAVDRLIFTPDQVDLARSPLRQGLPLGSTFVLGAFNPGL
                     AVLPTGNLLMMVRVAEALSRPIEGRVIRAIRWTPEDGYHLASWPLVGVDTSDPRKFEL
                     TGHAVRLLALTSMSWLLPVELTPDGLTVVAVHYDRAIGPRASWQDYGVEDARISRIGG
                     TWFMTTCSVSAERHATALYTSGDALDWTLRGIVLDHQNKDMLLFEGLVDGAFWALTRP
                     LGSLYFAFPPESPYRGGPSINLATSPDALHWKPVEQGGIRPRKVSQASEKVGGGAQPV
                     LTPEGWLVLYHGVEAGGPVGTYRTFWALLDARDPTRVLRLEDQTAILEPAPALTAPLR
                     DRMYLDGVVFTTGLADGGDHWIVLSGEADLACRVTHVPKTALV"
     gene            complement(2756..3544)
                     /locus_tag="PLO65_07560"
     CDS             complement(2756..3544)
                     /locus_tag="PLO65_07560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28140.1"
                     /translation="MSESAAAEPRTFARRDVHTEEPSLAGKRILITGGTTGIGRAVAA
                     LLGSCGARTFIFGRHAPELRDALEHIRAAGGAAEGVIADQAGADDIDKVFSAVDEALG
                     GLDVAIINAGLGSEPLSQAAEARWRYVVETNLTGAIAVAQEAAKRLEGRDGQGQIVLV
                     GSISADAEGEGSPAYAATKGGIRSFARSLRNELAKKGVRVSLIEPGTVGTDMIEDGPA
                     EQRARIGGHQMLRAEDIAVAIRYVVSQPPRCDVTSLRIEPRLQG"
     gene            complement(3619..4398)
                     /locus_tag="PLO65_07565"
     CDS             complement(3619..4398)
                     /locus_tag="PLO65_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD28141.1"
                     /translation="MTEPVAIRPAATILLVRDDPTFEVLMVKRHHQIDFASGALVFPG
                     GKTHAGDHDEAWADHVTGWDAIPAEKRPLRIAAIREAYEEAAIIIANDAAGRPFTGDD
                     RAGAARESVSRDERPFLDLVRELDVKLDLAALTTFARWITPDMMPKRFDTWFYLVRAT
                     GEQLAVCDGYETVDAEWIRPAEALRLEQAGERTIIFPTRMNLKLLAEATSAQDAIDRA
                     AARTLVPVLPKVERREGGGVLVIPEDAGYGAVVEPLSNIRA"
     gene            complement(4439..5578)
                     /gene="spbR"
                     /locus_tag="PLO65_07570"
     CDS             complement(4439..5578)
                     /gene="spbR"
                     /locus_tag="PLO65_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pole-localized protein SpbR"
                     /protein_id="HRD28142.1"
                     /translation="MGTTRAALTDVDLRTLLKGATADERAVAAHKLCRTIDREALTDD
                     ERAMAGDILRVMAADAAELVRRALAVTLKASDVVPHDVALKLARDVESISLPMLNFSP
                     VFSDDDLAEIVRLGGPVRQLAIARRPSLSERVTSAIVEHGSETAVETACANDNAAFSE
                     RILQRALDRFEKSEQVLAAVAYRNALPLAVTERLITMVGDQVRDHLLNHHALSPEKAL
                     TIAMAATERATVDLVDQAGRAADPKAFAAHLHKAQRLSASLLLRSLAHGHMTFFECAI
                     AELAGVPHHRTWLMIHDAGPLGLRAIYDRAGLPARLFAAFRAGVDTWHAMEFDGGLRD
                     RERFQERMLQRFLTQPQTVARDDVDYLIDKMDRISRESRRAVDVA"
     gene            5721..>6136
                     /locus_tag="PLO65_07575"
     CDS             5721..>6136
                     /locus_tag="PLO65_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28143.1"
                     /translation="MFNLPDPARTPRGGESVTLQSLEAQRGLLPYALALFGVSLPIYV
                     WAGSFAPNSVWMAASFAVFAINWGVFYAVVNWLRTDESRDLVRRGRVQILAGLLWSGA
                     CAQIAAFAEGAGPAREALLMVSVAGAVACFFFTAPSL"
BASE COUNT          917 a         2214 c         2089 g          916 t
ORIGIN      
        1 acatccgccg ggcgatcgcc agcgatcgtc ccgaactgct cgccctggcc tgcgccgcca
       61 tcggcgtcga ccgcggcgcc tttccgacca ttctcgaggc cgtccgcgac ctgaaccagg
      121 gccggccggg cggcggcgcc gaggccggac gccgcgccac cggcgccttc ggcccgttcg
      181 acgcggatat cgccggcgtc gccttccgca aggccgtgtc caccgtttga caaggccggc
      241 ggcataaggc cttgctcgcc ggaccatgtt caactccaag ccccacacca gccgcgtcac
      301 cttcctcgcc agcgaccgcc ccgaggccca ggaggcgcgc gagcgcctga tcgcccgcta
      361 cggcgacgcc ggcgacagcg cccaggtgat cgtcgccctg ggcggcgacg gcttcatgct
      421 cgagacgctg cacggcgccc tcgccgacgg aaagccgatc tacggcatga accgcggctc
      481 ggtcggcttc ctgatgaacg aatacagcga ggacggcctg ctcgagcgga tcagcgccgc
      541 cgagcgcgcc gtcatccatc cgctggcgat ggtcgccatc gacagcaagc gcaagcagca
      601 ccgggccctg gcgatcaacg aggtgtcgct gctgcgccag acccgccaga cggccaagct
      661 gcggatctcc atcgacggca aggtgcgcat gggcgagctg gtctgcgacg gggcgctggt
      721 gtcgaccccg gccggctcga ccgcctacaa cctgtcggcg cacggcccga tcattccgat
      781 cgacgcgcgg gtcatggccc tgacgccgat cagcgccttc cggccgcgac gctggcgcgg
      841 ggccctgctg tcgcacaccg cccgggtgac cttcgaggtg ctggaatccg acaagcggcc
      901 ggtcagcgcc gtggccgaca acgtcgaggt ccgcgacgtg ctcgaggtcc acatcgccga
      961 ggaccggtcg gtcagcctgg cgatgctgtt cgacgccggg cgcagtctgg aggagcgcgt
     1021 tctggccgag cagttctccg cctgatgcga cgacgcgccg cgcggaccgg aaatgctcgt
     1081 ctcatccgtt gttaaccccg atcgactagc gtcctccggg tgaaacgcca gaggaggcga
     1141 tgcgtgagcg acaatcccac ggtccaggtc atccaggccc cgaacactct gcgcctcaag
     1201 gtgggcggcc ggctgggcgg gatcgacccc gccgccatcg ccaaggcgga agccgcgctc
     1261 aagagccttt cgggtaattt cagcgagtgg ctcaatgacg agctggtcaa gctcgaggcc
     1321 gcccggcagc gcattcgcgc cgaaggtctc aacaccgaga cggccgaggg tctgtatctg
     1381 cgcgcccatg acctcaaggg ccttggcgcc acatacgaat tcccgctggt cacccgcatc
     1441 gccggctcgc tgtgcaagct gatcgatgac ccggacaccc ggctggacgc cccgatgttc
     1501 ctggtcgacg cccatatcga cgggatcaag gcctcggtcc gcggcaacat ccgcagcgag
     1561 gatcatccgg tcggccgcaa gctggtcgag gaactcgaag accgggtcgc caagctcgcc
     1621 gcctagacca acgccgtctt cgggacatgc gtcacccggc aggccagatc ggcctcgccg
     1681 gacagcacga tccaatggtc gccgccatcg gcgagtccgg tcgtgaacac caccccatcc
     1741 aggtacatgc ggtctcgcag cggcgcggtc aaggccggtg cgggctcgag gatcgccgtc
     1801 tggtcctcga gccgcagaac gcgggtcgga tctcgcgcgt cgagcagggc ccagaaggtg
     1861 cgataggtcc ccacgggacc gcccgcctcg acgccgtgat agagcaccag ccagccttcg
     1921 ggcgtcagca ccggctgggc tccgccaccg accttctcgc tcgcctgcga gaccttccgg
     1981 gggcgaatgc cgccctgttc gaccggcttc cagtgcagcg cgtcaggcga cgtggccagg
     2041 ttgatcgaag ggccgccgcg atacgggctt tccggcggaa aggcaaaata cagactgccg
     2101 agcggccgcg tcagcgccca gaaggcgccg tcgaccagac cctcgaacag cagcatgtcc
     2161 ttgttctggt gatccagcac gatgccgcgc agggtccagt ctagcgcgtc gcccgaggtg
     2221 tagagggcgg tggcgtggcg ttcggcgctg acgctgcagg tggtcatgaa ccaggtcccg
     2281 ccaatccggc tgatgcgcgc gtcctccacg ccatagtcct gccaggacgc gcgcgggccg
     2341 atggcccggt cgtagtggac ggccaccacc gtcagcccgt caggggtcag ttcgaccggt
     2401 aacagccagg acatcgaggt cagggccagc aggcgaaccg catgcccggt cagttcgaac
     2461 ttccgcgggt cgctggtgtc gaccccgacc agcggccagc tggccagatg gtagccgtcc
     2521 tccggtgtcc agcggatcgc gcggatgacg cggccctcga tcggccggct cagcgcctcg
     2581 gccacgcgca ccatcatcag cagattgccc gtcggcagca cggccaggcc ggggttgaag
     2641 gcccccagca cgaaggtcga gccgagcggc agaccctggc gcaggggcga gcgggccagg
     2701 tccacctggt cgggggtgaa gatcaggcgg tccacggcgt aggtcggcgg catcatcagc
     2761 cctgcagccg cggctcgatg cgcagcgagg tcacgtcgca gcgaggcggc tggctgacca
     2821 cgtagcggat ggccacggcg atgtcctcgg cgcgcaacat ctggtggcca ccgatccggg
     2881 cgcgctgctc ggccggcccg tcctcgatca tgtccgtccc caccgtcccc ggctcgatca
     2941 ggctgacgcg gacccctttc ttcgccagtt cgttgcgcaa tgacctggcg aatgaacgga
     3001 tgccgccctt ggtcgccgcg taggccgggc tgccctcgcc ctccgcgtcg gcgctgatcg
     3061 agccaaccag cacgatctgc ccctggccgt cccggccctc gagccgcttc gcggcctcct
     3121 gagccacggc gatcgccccc gtgaggttcg tttccaccac atagcgccag cgggcctccg
     3181 cggcctgcga cagcggctcg ctgcccaggc cggcgttgat gatggcgaca tccagtccac
     3241 ccagcgcctc gtcgacggcg gaaaacacct tgtcgatgtc atcggccccg gcctggtcgg
     3301 cgatcacccc ctccgccgcg cccccggcgg cgcgaatgtg ctcgagcgcg tcacgcaact
     3361 ccggcgcatg gcggccaaag atgaaggtgc gtgccccgca ggatccgagc agcgccgcga
     3421 cggcgcggcc gatacccgtc gtgccgccgg tgatcaggat gcgcttgccc gccagcgacg
     3481 gctcttcagt gtgaacgtcg cgccgcgcga aggtgcgcgg ttcggcggcg gcggattcgg
     3541 acatcggcgg gctcccgggg gctgtccgcc ttcaacggct gcccccgccg ggccgttccg
     3601 ccgcccgtga cgcggggctc aggcccggat gttcgacagc ggctcgacca cggcgccgta
     3661 accggcgtct tccggaatga ccagcacgcc gccgccctcg cggcgttcga ccttcggcag
     3721 caccggcacc agggtgcggg cggcggcgcg gtcgatggcg tcctgggcgc tggtcgcctc
     3781 ggccagcagc ttcaggttca tgcgggtcgg aaagatgatc gtccgctcgc ccgcctgttc
     3841 aagacgcagg gcctcggcgg gccggatcca ctcggcgtcg accgtctcgt agccgtcgca
     3901 taccgccagc tgctcgcccg tggcccgcac aagatagaac caggtgtcga accgcttggg
     3961 catcatgtcg ggggtgatcc agcgggcgaa ggtcgtcagg gccgccaggt cgagcttcac
     4021 atccagctcg cgcacgaggt ccagaaacgg ccgctcgtcc ctcgacacgg attcgcgggc
     4081 ggcgccggcg cggtcgtcgc cggtgaaggg ccggccggcg gcgtcgttgg cgatgatgat
     4141 cgccgcctcc tcgtaggcct cgcgaatcgc cgcgatgcgc agcggacgct tctcggccgg
     4201 tatcgcatcc caacccgtca catggtcggc ccaggcctcg tcgtggtccc cggcgtgggt
     4261 cttgccgccc gggaacacca gcgcgcccga ggcgaaatcg atctggtggt gccgcttgac
     4321 catcaggacc tcgaaggtcg ggtcgtcacg gacgagcagg atggtggcgg cggggcgaat
     4381 ggcgacgggc tcggtcatga ggcgaaggat cgcgcctcaa acacccgttc ggcaagcctc
     4441 acgccacgtc aacggcccga cggctctcgc gcgagatacg gtccatcttg tcgatcagat
     4501 agtcgacgtc gtcccgggcc acggtctggg gctgggtcag gaaccgctgc aacatccgct
     4561 cctggaagcg ttcgcggtcg cgcagcccgc cgtcgaactc catcgcatgc caggtgtcga
     4621 ccccggcgcg gaacgcggcg aacagccgcg ccggcaggcc ggcccggtca tagatggcgc
     4681 gcaggcccag cggcccggcg tcgtggatca tcagccacgt gcggtgatgc ggcacgcccg
     4741 ccagctcggc gatggcgcac tcgaagaagg tcatgtggcc gtgcgccagc gagcgcaaca
     4801 gcagcgaggc ggacagccgc tgggccttgt gcaggtgggc ggcgaaggcc ttgggatcgg
     4861 cggcccggcc ggcctggtcg accaggtcga ccgtcgcccg ctcggtggcg gccatggcga
     4921 tggtcagcgc cttttccggc gacagcgcgt gatggttcag caggtgatcg cgcacctggt
     4981 cgccgaccat ggtgatcagg cgctcggtga ccgccagcgg cagggcgttg cgataggcga
     5041 ccgccgccag cacctgctcg gacttctcga accggtccag cgcccgctgc aggatgcgtt
     5101 cggagaaggc cgcgttgtcg ttggcgcagg cggtctcgac cgccgtttcc gagccgtgct
     5161 cgacaatcgc gcttgtgacc cgttccgaca ggctgggccg cctggcgatg gcgagctggc
     5221 gcaccggccc gccgagccgg acgatctcgg ccaggtcgtc gtcgctgaac accggcgaga
     5281 agttcagcat cggaagggag atcgactcga cgtccctggc cagcttcagg gcaacgtcat
     5341 gcgggaccac gtccgaggcc ttcagcgtca ccgccagggc ccggcgcacc agctcggcgg
     5401 cgtcggcggc catcacccgc aggatgtcgc cggccatggc ccgttcatcg tcggtcaggg
     5461 cctcgcggtc gatggtccgg cacagcttgt gcgcggccac cgcccgctcg tccgccgtcg
     5521 cccccttgag aagggtgcgc aggtccacat ccgtcagtgc tgcccgtgtc gtgcccatgt
     5581 cgaatcccgc ccggcgtcgt cgacgcggct gtgatcatga gttgttaaac ttaacgaagg
     5641 ctgaccgacc attaactacc tcccttcacc ccccgcaaac gcccctcgcc tagacttcgc
     5701 gcgccagaag aggggatgcc gtgttcaacc tgcccgaccc cgcccgcacg ccccgcggcg
     5761 gagagtccgt caccctccag tcgctcgagg cccagcgcgg cctgctgccc tatgccctgg
     5821 cgctgttcgg ggtcagcctg ccgatctacg tctgggccgg cagcttcgcg cccaacagtg
     5881 tgtggatggc cgccagcttc gcggtgttcg cgatcaactg gggcgtcttc tacgccgtgg
     5941 tgaactggct gcggaccgac gagagccgcg acctcgtccg gcgcggccgg gtgcagattc
     6001 tcgccggcct gctgtggtcg ggcgcctgcg cccagatcgc cgccttcgcc gagggcgccg
     6061 gaccggcgcg ggaggccctg ctgatggtct ccgtggccgg cgccgtggcc tgtttcttct
     6121 ttaccgcccc gagcct
//
LOCUS       DAORNX010000139         6092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9613_length_6092_cov_7.967037, whole genome shotgun sequence.
ACCESSION   DAORNX010000139 DAORNX010000000
VERSION     DAORNX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_6092_cov_7.967037"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..577)
                     /locus_tag="PLO65_07580"
     CDS             complement(110..577)
                     /locus_tag="PLO65_07580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD28144.1"
                     /translation="MTTDAENTLLLTTDTGTVTIKLRPDLAPGHVERIKELAREGFYD
                     GVVFHRVIPGFMAQGGDPTGTGSGGSKKPDLKAEFSNEKHLRGVCSMARTSYPHSANS
                     QFFICFDDATFLDGQYTVWGEVVDGMDHIDALPKGEPPRQPGRIVSMKVAADA"
     gene            complement(582..1415)
                     /locus_tag="PLO65_07585"
     CDS             complement(582..1415)
                     /locus_tag="PLO65_07585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD28145.1"
                     /translation="MRSTLLLVALLSLTAPAALARPAAPTAADWRTPDPQNVLVIDTS
                     KGRVVVELVPEVAPGHVERLRVLARKGAYDGRTFFRVIDRFMAQTGDPQDTGEGGSDL
                     PNLKAEFTFRRDAASGFVTAASPWGTEEGFIKALPVVSQAWSWSEMTQDGKVAAWGSY
                     CPGVVGMARDENPDSANSQFFLMRQPYLSLDKRYTAFGRVISGLEAIRAIKTGEPVAA
                     PQDRMDHVRVLADIPAAERPTVRVIDPAGPWFKAEIQRVRQLKGADFSLCDVNFPAEV
                     N"
     gene            complement(1427..2302)
                     /locus_tag="PLO65_07590"
     CDS             complement(1427..2302)
                     /locus_tag="PLO65_07590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD28146.1"
                     /translation="MKFAPTALAALLFAAAPALAARAAGPGVPEPSAAEWRTPDPETI
                     MVIDTNKGRMIVELSPWAAPVNAERVRLLARQGFYDGLTFFRVIDDFMAQTGDPRNDG
                     TGGSDALPDMAAEFTFRRGRDLPFTSFGRLPADNDTPSTTEVGFINGFAVRGAPQMQM
                     MASADGKVPGWPLFCAGVLGMARSTQPDTANSQFFIMRHTYPLLDGNYTVVGRVLGGP
                     EVVRAIKTGEPVPDPRDVMTRVRMLADIPAAERPKVQVLDTAGPAFKAMLEAARAAGA
                     GPAKACDIAVPVRIG"
     gene            complement(2408..2902)
                     /gene="coaD"
                     /locus_tag="PLO65_07595"
     CDS             complement(2408..2902)
                     /gene="coaD"
                     /locus_tag="PLO65_07595"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRD28147.1"
                     /translation="MARVGLYPGTFDPMTNGHLDIIGRAVKLVDKLVIGVAINEGKGP
                     LFSLEERVAIVRDETAHLRKIAEIEVIPFKGLLMHFAREVHAGIIVRGLRAVADFEYE
                     FQMTAMNQQLDREIETVFLMADPRHQAVASRLVKEIAILGGDITKFVTPGVATRLLAK
                     VGRE"
     gene            complement(2972..5713)
                     /gene="gyrA"
                     /locus_tag="PLO65_07600"
     CDS             complement(2972..5713)
                     /gene="gyrA"
                     /locus_tag="PLO65_07600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRD28148.1"
                     /translation="MTDETNTPPEDARRGAVSPIAIEDELKRSYLDYAMSVIVSRALP
                     DVRDGLKPVHRRILFAMNENGFTSNAAYRKSARTVGDVIGKYHPHGDVAVYMALVRMA
                     QPFSMGVMLIDGQGNFGSVDGDMPAAMRYTESRLTRAAEALLADLDKDTVDFQENYDG
                     SENEPTVLPARIPNLLVNGAGGIAVGMATNIPPHNLGEIVDACLAFIDNPDIPLDELL
                     DIVPGPDFPTGGELIGRTAARQALMTGRGSVIIRGVASVEELRKDREGIVITAIPFQV
                     NKSSLVEHIADLVRDKKLEGISDIRDESDRAGMRIVVELKRDAQGDVILNQLYRFTAL
                     QSSFSYNMLALNRGRPEQMGLREMITAFVEFREEVVVRRTRFELGKARDRGHVLVGLA
                     IAVANIDEFIRIIRSSVDPAEARERLCGQDWPTGDMMPLIELIADPRTVITGGDQVRL
                     SDEQARAILALTLSRLTGLGRDEIMGEARTLAGSIAELLELLSDRANIMAVVRGELVE
                     VREKFAIPRRTVFGEGGADMDDEDLIPREDMVITVTHGGYVKRTPLSTYRSQRHGGKG
                     RSGMSTKDEDAVTRVFSASTHAPVLFFSSGGKVYKLKVWRLPMGAPTSRGKAFVNLLP
                     IEPGEAITSILPLPEDEEQWDQLDVMFATRSGNVRRNKLSDFVQINRNGKIAMKLDEG
                     DSIVGVGLCTAANDVLLTTALGRAIRFEVGEVRVFAGRDSTGVRGIRLADGDEVLSMA
                     ILHGVDATPAERAAYLKYERARLRAESADDEDVATPADDEDEAEEAADLTPERIIALQ
                     AAEEYILTLSSEGIGKRSSAYDFRRTGRGGQGLLAQDLTRKGGRLVASFPVDQADEIL
                     LVTDAGQLIRCPVSGIRIAARNTQGVIVFRTAEDEHVVSVERLEASGEGVEDADSGE"
     gene            5968..6092
                     /locus_tag="PLO65_07605"
                     /pseudo
     CDS             5968..6092
                     /locus_tag="PLO65_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
BASE COUNT          896 a         2051 c         2128 g         1017 t
ORIGIN      
        1 cgcgtggttc gacacgcccg ctgcgcgggc tgctcaccac gacgtgcgcg gaatcacgtc
       61 atcctgagca gccccggcgc tgagccgggg cgcgtcgaag gacgcacgat cacgcgtccg
      121 cggcgacctt catcgagacg atacggcccg gctggcgggg cggctcgccc ttgggcaggg
      181 cgtcgatgtg gtccatcccg tccaccacct cgccccacac cgtgtactgg ccgtcgagga
      241 aggtggcgtc gtcgaaacag atgaagaact ggctgttggc cgagtgcgga tagctggtgc
      301 gcgccatcga gcagacgccg cgcaggtgct tttcgttgga gaactccgcc ttcaggtcag
      361 gcttcttcga accgcccgag ccggtgcccg tggggtcgcc gccctgggcc atgaagccgg
      421 ggatcacgcg gtggaagacc acgccgtcgt agaagccctc ccgcgccagt tccttgatcc
      481 gctcgacatg gccgggggcc aggtcggggc gcagcttgat ggtgaccgtg ccggtgtcgg
      541 tggtcagcag cagggtgttt tcggcgtcgg tcgtcatcgt ttcagttcac ttccgcagga
      601 aagttgacgt cgcagagcga gaaatccgcg cccttaagtt ggcgcacgcg ttgaatctcg
      661 gccttgaacc aggggcccgc tgggtcgatg acccggaccg tcggccgttc ggcggcgggt
      721 atatcggcca gaacgcgcac gtggtccatc cgatcctgcg gcgcggcgac cggttcgccg
      781 gtcttgatcg cgcggatcgc ttccagtccg ctgatcaccc ggccgaacgc cgtgtaacgc
      841 ttgtcgagcg acaggtaggg ctggcgcatc aggaagaact ggctgttggc gctgtcggga
      901 ttctcgtcgc gggccatgcc gaccacgccc gggcagtagc tgccccaggc cgcgaccttg
      961 ccgtcctggg tcatctcgct ccagctccag gcctggctga ccacgggcag cgccttgatg
     1021 aagccctcct cggtccccca cgggctggcg gcggtgacga agcccgaggc ggcgtcgcga
     1081 cggaaggtga actcggcctt caggttgggc aggtcgctgc ccccctcccc ggtgtcctgc
     1141 ggatcgcccg tctgggccat gaaccggtcg atcacgcgga agaaggtgcg gccgtcatag
     1201 gcgcccttgc gggccagcac gcgcaggcgc tcgacatggc ccggggccac ttccgggacc
     1261 agctcgacca ccacccggcc cttgctggtg tcgatcacca gcacgttctg cggatcgggt
     1321 gtgcgccagt cggcggccgt cggggccgcg ggtctcgcca gggccgccgg agcggtcagc
     1381 gacagcaggg cgacgagcag cagggtcgaa cgcatggcgg gtctcctcaa ccgatcctga
     1441 ccgggacggc gatgtcgcag gccttggccg gcccggcgcc ggcggcccgg gcggcctcga
     1501 gcatggcctt gaacgcggga ccggcggtgt cgagcacctg gaccttcggc cgctcggcgg
     1561 cggggatgtc ggccagcatg cgcacccggg tcatgacgtc gcgcggatcg ggaaccggct
     1621 cgccggtctt gatcgcccgc accacctcgg ggccgccgag cacacggccg acgacggtgt
     1681 agttgccgtc gagcagcgga taggtgtgcc gcatgatgaa gaactggctg ttggcggtgt
     1741 cgggctgggt cgaccgggcc atgcccagca cgccggcgca gaacaacggc cagccgggca
     1801 cctttccgtc ggccgaggcc atcatctgca tctgcggcgc gccccgcacc gcgaagccgt
     1861 tgatgaaccc gacctcggtc gtcgagggcg tgtcgttgtc ggccggcagg cgaccgaatg
     1921 acgtgaacgg caggtcgcgg ccacgccgga aggtgaactc ggccgccatg tccggcaggg
     1981 cgtccgatcc gccggtgccg tcgtttcgcg ggtcgcccgt ctgggccatg aagtcatcga
     2041 tcacccggaa gaaggtcagt ccgtcataga atccctgacg ggccagcagc ctgacgcgct
     2101 cggcattgac cggcgcggcc cagggcgaca gctcgacgat catgcgaccc ttgttggtgt
     2161 cgatgaccat gatggtttcg ggatcgggcg tgcgccactc ggcggccgag ggttccggaa
     2221 ccccgggccc ggcggcgcgg gcggccaggg ccggcgcggc ggcgaacagg agggcggcga
     2281 gcgcggtcgg cgcgaatttc atgcggtcac tctttcaaaa ggccccgtca gcccgcgtag
     2341 aacggcagag ggaccagaac aagtccgtac ggcgatcgcg gtgccggtcg gtcgcgaccg
     2401 accggcccta ttcccggccg accttggcga gcagtcgggt ggccacgccg ggcgtgacga
     2461 acttggtgat gtcgccgccg aggatggcga tctccttgac cagccgcgag gcgacggcct
     2521 ggtggcgcgg gtcggccatc aggaacaccg tctcgatctc ccggtcgagc tgctggttca
     2581 tggcggtcat ctggaattcg tattcgaagt cggccacggc ccgcaggcca cggacgatga
     2641 tgcccgcgtg gacctcgcgg gcaaagtgca tcaggaggcc cttgaacggg atcacctcga
     2701 tttcggcgat cttccgcagg tgggcggtct cgtcgcggac gatggccacc cgctcctcca
     2761 gggagaacag cgggcccttg ccttcgttaa tggccacgcc gatgaccagc ttgtccacca
     2821 gcttcacggc ccggccaatg atgtccagat gaccgttggt catcggatcg aacgtgcccg
     2881 gatacagtcc gactcgcgcc atcgccgccc ctttcgatgc tgcagcgcac cctagctcag
     2941 tagcgcgacg acgcaaggcg ggtcgggagg gctattctcc gctgtcggcg tcctcgacgc
     3001 cctcgccgga agcctccagc cgctccacgc tcacgacgtg ttcatcctcg gcggtgcgga
     3061 agacgatcac gccctgggtg ttgcgcgccg cgatgcggat tccgctgacc gggcagcgga
     3121 tcagctggcc cgcgtcggtg accagcagga tctcgtcggc ctgatcgacc gggaagctgg
     3181 cgaccaggcg gccgcccttg cgggtcaggt cctgcgccag cagtccctga ccgccgcggc
     3241 ccgtgcggcg gaaatcgtag gcgctgcttc gcttgccgat gccctcggac gacagggtga
     3301 ggatgtattc ctccgccgcc tggagtgcga tgatgcgctc gggagtgaga tcagcggctt
     3361 cctcggcctc gtcctcgtcg tccgccggcg tggcgacgtc ctcgtcatcg gcgctttcgg
     3421 ccctcaggcg cgcgcgctcg tacttgagat aggccgcgcg ttccgccggc gtcgcatcga
     3481 cgccgtgcag gatggccatg gacagcacct catcgccgtc cgccagcctg atgccgcgca
     3541 cgccggtgga gtcgcggccg gcgaacaccc gcacctcgcc cacctcgaac cggatcgccc
     3601 gaccgagcgc ggtggtcagc agcacgtcat tggcggccgt gcacaggccc acgccgacga
     3661 tgctgtcccc ctcgtccagc ttcatggcga tcttgccgtt gcggttgatc tgcacgaagt
     3721 cgctgagctt gttgcggcgg acgttgccgc tgcgggttgc gaacatcacg tccagctgat
     3781 cccactgctc ctcgtcctcg ggcagcggca ggatcgaggt gatggcttct cccggctcga
     3841 tcggcagcag gttgacaaac gccttgccgc gcgaggtcgg ggcgcccatc ggcaggcgcc
     3901 agaccttgag cttgtagacc ttgccgcccg agctgaagaa caggaccggg gcgtgggtcg
     3961 aggcggagaa cacgcgggtg accgcgtcct cgtccttggt cgacatgccc gagcggcctt
     4021 taccgccgtg gcgctggctg cggtaggtcg acagcggcgt gcgcttcaca tacccgccgt
     4081 gggtgacggt gatgaccatg tcctcgcgcg ggatcaggtc ctcgtcgtcc atgtccgcgc
     4141 cgccctcgcc gaagacggtg cggcgcggga tggcgaactt ctcgcgcacc tcgaccagct
     4201 ccccgcggac cacggccatg atgttggccc ggtcggacag cagctcaagc agttcggcga
     4261 tcgagccggc cagcgtgcgg gcctcgccca tgatctcgtc gcggccaagg ccggtcaggc
     4321 gcgacagcgt cagggccaga atggcgcggg cctgttcgtc ggacagtcga acctggtcgc
     4381 cgccggtgat caccgtgcgc ggatcggcga tcagctcgat cagcggcatc atgtcgccgg
     4441 tcggccagtc ctggccgcac aggcgctcgc gcgcctcggc cggatcgacc gaggagcgga
     4501 tgatgcggat gaattcgtcg atgttggcca cggcgatggc cagaccgacc agcacatggc
     4561 cgcggtcgcg ggccttgccc agctcgaagc gggtccggcg gacgaccacc tcttcgcgga
     4621 actccacgaa ggcggtgatc atctcgcgca ggcccatctg ctcgggccgt ccgcggttga
     4681 gcgccagcat gttgtagctg aagctgctct gcagggccgt gaagcgatag agctggttga
     4741 ggatcacgtc gccctgggcg tcgcgcttga gctcgacgac gatgcgcatc ccagcgcgat
     4801 cgctctcgtc gcggatgtcg ctgatgcctt cgagcttctt gtcgcggacc aggtcggcga
     4861 tgtgctcgac caggctggac ttgttcacct ggaacgggat cgccgtgatg acgatgccct
     4921 cgcggtcctt gcgcagctcc tcgacgctgg ccacgccgcg gatgatcacc gagccgcggc
     4981 cggtcatcag ggcctggcgg gcggcggtgc ggccgatcag ctcgccgccg gtcgggaagt
     5041 ccggccccgg cacgatgtcg agcagctcat cgagcggaat atcggggttg tcgatgaagg
     5101 ccaggcaggc gtcgacgatt tcgcccagat tgtgcggcgg aatattggtc gccatgccca
     5161 cggcgatgcc accggcgccg ttgaccagca ggttgggtat ccgcgccggc aacaccgtcg
     5221 gctcgttctc ggagccgtcg tagttttcct ggaagtcgac cgtgtccttg tcgagatccg
     5281 ccagcagcgc ctccgccgcc ctggtcaggc gggattcggt gtagcgcatg gccgccggca
     5341 tatcgccgtc gaccgagccg aagttgccct ggccgtcgat cagcatgacg cccatcgaga
     5401 acggctgggc catccgcacc agggccatgt agacggcgac gtcgccgtgc gggtgatact
     5461 tgccgatcac gtcgccgacg gtgcgggccg acttgcggta ggccgcattc gacgtgaagc
     5521 cgttctcgtt catcgcgaac aggatgcggc ggtgcaccgg cttgaggccg tcgcggacgt
     5581 ccggcagggc gcggctgacg atcacgctca tggcgtagtc gaggtagctg cgcttgagtt
     5641 cgtcctcgat ggcgatgggg cttacggccc cgcgccgggc gtcttccgga ggggtattgg
     5701 tttcgtcggt caagtgcggg aatttgctgt cagaatcgga caggaagcgt ccattcccta
     5761 gttaacattc gcatccgccc ggcgccatct ttcgggcggc tttttcgggc tgatggcgcg
     5821 tcgaaacccg tgatccgccg cgatattacc gatgctgcaa ggagcaattc gatgcgccgc
     5881 ctgatcctcc ccctcgccat cgccgccgtc ggcctcccgg ccgccgccgg acccgcgccg
     5941 gtcaccgtcc agctgaaggg accggccggc gaggacatgg gctcggccat gctgaccgag
     6001 gccccgcatg gcctggtgct gcgggtcgcg gccagggggc tgacgcccgg ctggcacggc
     6061 ctgcacttcc acgagaaggc cgactgctcg ag
//
LOCUS       DAORNX010000140         6090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9616_length_6090_cov_7.186081, whole genome shotgun sequence.
ACCESSION   DAORNX010000140 DAORNX010000000
VERSION     DAORNX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_6090_cov_7.186081"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLO65_07610"
     CDS             <1..786
                     /locus_tag="PLO65_07610"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRD28149.1"
                     /translation="GPSGRPPPAPAVRARTRYEGDFAYFRLVADLMAENPPPAKHVGM
                     LAMMRRIGLVPGKPFDPARLDPATRAGVLRAEKEGPAIIDLIRRTRGTPTASGWTANR
                     AALANDFDYPTRAGNALVGLMGNDPEEALYFNNFFGADGQALGGGRAYRLHFKAEDIP
                     RTDALGFWSLTLYDARRFQFVANPLDRYALGSRDDLVRNADGSVDIYVQPTAPGGARD
                     RNWLPSPASGDFKVTLRIYMPTPGMVAAYRQSSYLAPAVEPVE"
     gene            848..>1703
                     /locus_tag="PLO65_07615"
     CDS             848..>1703
                     /locus_tag="PLO65_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD28150.1"
                     /translation="MAAEPTSPAAPSAPGDSKALYVLLAVVFVNIAGFGVIIPLLPFY
                     AKSFGAPDWQVFVLFACYSVGNFFAEPLWGRLSDRIGRRPVLMMTIFGNALTYVLLAF
                     APNLWVACAIRLTGGVLTGNISTIQGYMADVTPPDRRAGRLGLMGAAFSLGFMTGPSI
                     GGLLAHADQGTIGFRPPLLFAATLALAAGVGITLFVRETRHAHKDAPKRGRLEGLGDA
                     AAHPVIWRTLLVTLISMAGFAGMEATFGLWAHAKFDWGPRQIGFCFFAIAITAAGAPG
                     FLTGFMARR"
     assembly_gap    1704..1722
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1723..2062
                     /locus_tag="PLO65_07620"
     CDS             <1723..2062
                     /locus_tag="PLO65_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD28151.1"
                     /translation="GLALIFAGFALQPLVHAWPLAVVGMVTVALGQSLTFPNIGAIIS
                     KSASPERQGEMMGLNTAAGALARIIGPLMAAPLFAGFGPSAPFAVAAGLCLPALFMAW
                     QVSKAVRRSA"
     assembly_gap    2077..2161
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2350..2550
                     /gene="rpmI"
                     /locus_tag="PLO65_07625"
     CDS             2350..2550
                     /gene="rpmI"
                     /locus_tag="PLO65_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRD28152.1"
                     /translation="MPKLKTKSGAKKRFKLTATGKLKAGVAGKRHRLISHNGKYIRQQ
                     RGTKVMSASDSKTIKTYLPYGL"
     gene            2567..2923
                     /gene="rplT"
                     /locus_tag="PLO65_07630"
     CDS             2567..2923
                     /gene="rplT"
                     /locus_tag="PLO65_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRD28153.1"
                     /translation="MARVKRGVTAHAKHKKVLEQAKGFYGRRKNTIRTAKAAVDKAGQ
                     YAYRDRKVRKRSFRSLWIQRINAAARLEGFTYSQFIHGLDQAGVEIDRKVLADIAGQD
                     PTAFKAIADKVRAALA"
     gene            3162..4274
                     /gene="pheS"
                     /locus_tag="PLO65_07635"
     CDS             3162..4274
                     /gene="pheS"
                     /locus_tag="PLO65_07635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRD28154.1"
                     /translation="MTDLTTLETDLTAQIAAAPDVAALEAVRVAALGKSGSISGLLKT
                     LGAMSPDERREAGPAINGLRDRVAAAIAGRKAALEAAELDARLAAETLDLTLPAPPRR
                     RGTVHPTMQVMDEMTAIFAEMGFAVAEGPDIETDFNNFTALNFPEKHPAREMHDTFFF
                     KPGPDGERKLLRTHTSPVQVRAMQRTNQPPPAPWIATAIEPPIRIVVPGRTYRCDSDA
                     THSPMFHQMEGLVIDKGIHMGHLKWTLETFIARFFEVEGVVSRFRPHHFPFTEPSAEM
                     DIGCDRSGGEIKIGTGSDWLEILGCGMVHPNVLRFCGIDPDVYQGFAFGLGIDRLGML
                     KYGMPDLRDMFEGDTRWLGHYGFSAFAAPNPATGLS"
     gene            4274..4666
                     /locus_tag="PLO65_07640"
     CDS             4274..4666
                     /locus_tag="PLO65_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28155.1"
                     /translation="MASHLENIPWTLAKDLNAKAFDKEVVILSDLYRAAIQNGQSFIE
                     NRSFTDCRLEGPAVVAVVGGVSFDSTDFGYTGGDVGRIVWRTASTTGVVGAIPFRNCQ
                     FKGCSFFATGFTGPEAFLQQILALKTVP"
     gene            4663..>6090
                     /gene="pheT"
                     /locus_tag="PLO65_07645"
     CDS             4663..>6090
                     /gene="pheT"
                     /locus_tag="PLO65_07645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRD28156.1"
                     /translation="MKFTLSWLREHLDTEATTARVVEAMTMAGLEVEHVIDPATKLAP
                     FTVARIAEAVQHPNADRLRVCQVDTVDGRKEIVCGAPNARAGLTTIYAPIGSYVPGLG
                     VTLVEKPVRGVVSNGMLCSAAEMETAEESDGILELDDSLPVGTPAAQALGLEAVIDFE
                     VTPNRPDWLGVAGIARDLSAAGLGPLRDLAVKPVPGQFPSPIAVRVDGQACRVFAGRL
                     IRGVKNGPSPAWLQARLKAIGLRPINALVDVTNLIAYDRARPLHVYDAARLVGTTIEA
                     RLGHHSDHGEERLIALDGKTYDLGPEMSVIADADGERPIGLGGVMGGESTGCSDDTTE
                     VFVESAWFDPIVTAQTGRATGITSDAQYRFARGVDPGFVVDGLELATRLILELCGGEP
                     SDIVVAGEPPAPPAPVAFDPDYVRQLSGLTIPADRSAQILGTLGFAIDRAGPVWTVTP
                     PTWRRDVEGKADLVEEVMRIHGVDEI"
BASE COUNT          905 a         2195 c         1972 g          914 t
ORIGIN      
        1 gggccgtcgg gcaggccgcc gccggcgccc gccgtccggg cgcggacgcg ctacgagggc
       61 gacttcgcct atttccggct ggtcgccgac ctgatggcgg agaacccgcc gccggccaaa
      121 catgtgggca tgctggccat gatgcggcgc atcggcctgg ttcccggcaa gccgttcgac
      181 ccggccaggc tcgatccggc cacccgggcc ggcgtcctgc gcgccgagaa ggaggggccg
      241 gcgatcatcg acctgatccg gcggacgcgc gggacgccga ccgcctccgg ctggaccgcc
      301 aaccgggccg cgctggccaa cgacttcgac tatccgacgc gcgccggcaa cgccctggtc
      361 gggttgatgg gcaacgatcc cgaagaggcg ctctacttca acaacttctt cggcgcggac
      421 ggccaggcgc tgggcggcgg ccgggcctac aggctgcatt tcaaggcgga agacatcccc
      481 cggaccgacg ccctgggctt ctggtccctg accctctacg acgcccgccg cttccagttc
      541 gtcgccaatc cgctcgatcg ctacgccctg ggcagccggg acgacctggt gcgcaacgcc
      601 gacggctcgg tcgacatcta tgtgcagccc accgccccgg gcggcgcgcg cgaccgcaac
      661 tggctgcctt ccccggcgag cggcgatttc aaggtgaccc tgcggatcta catgccgacg
      721 cccgggatgg tcgccgccta ccggcagtcg tcgtacctcg cgccggccgt cgaaccggtg
      781 gagtgaaggg tggcggcggc ggcgccattt gccgccatcg ccgcaacggc ctaaaccccc
      841 gcccctgatg gccgccgaac cgacatcccc cgccgccccg tcggcgcccg gcgacagcaa
      901 ggcgctctac gtcctgctgg cggtggtgtt cgtcaacatc gccgggttcg gggtgatcat
      961 tcccttgctg ccgttctacg ccaagtcgtt cggggccccg gactggcagg tgttcgtgct
     1021 gttcgcctgc tactcggtgg gcaacttctt cgccgagccg ctgtggggcc ggctgtcgga
     1081 ccgcatcggg cggcggccgg tgctgatgat gaccatcttc ggcaacgccc tgacctatgt
     1141 gctgctggcc ttcgcgccga acctgtgggt cgcctgcgcc atccggctga ccggcggggt
     1201 gctgaccggc aacatctcga ccatccaggg ctacatggcc gacgtgacgc cgccggaccg
     1261 ccgggccggg cgactggggc tgatgggcgc ggccttctcg ctcggcttca tgaccggccc
     1321 gtcgatcggc gggctgctgg cgcacgccga ccagggcacc atcggcttcc gcccgccgct
     1381 gctgttcgcc gccaccctgg ccctggcggc gggcgtcggc atcaccctgt tcgtgcgcga
     1441 gacgcgccac gcccacaagg acgcgcccaa gcgcggacgg ctggagggcc tgggcgacgc
     1501 cgcggcccat ccggtgatct ggcgcaccct gctggtgacg ctgatctcga tggccgggtt
     1561 cgccggcatg gaggcgacct tcggcctgtg ggcccacgcg aagttcgact ggggacccag
     1621 gcagatcggc ttctgcttct tcgccatcgc catcaccgcg gcgggcgccc cgggcttcct
     1681 gaccgggttc atggcccggc gggnnnnnnn nnnnnnnnnn nncgggctgg ccctgatctt
     1741 cgccggcttc gccctgcagc cgctggtcca tgcctggcca ctggccgtgg tcggcatggt
     1801 caccgtggcg ctcggccagt cgctgacctt tcccaacatc ggggcgatca tttccaagtc
     1861 cgcctcgccc gagcggcagg gcgagatgat ggggctgaac accgccgccg gcgccctggc
     1921 gcggatcatc ggcccgctga tggccgcgcc gctgttcgcc gggtttggcc cgagcgcgcc
     1981 gttcgccgtg gcggcgggcc tgtgcctgcc cgcgctgttc atggcctggc aggtcagcaa
     2041 ggcggtcagg cggtcggcat aacctcccct tcccccnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 ngtgttcctg ggtggcccga acaagtcggg ccatgacgga ggtggaattg ccgccagtcg
     2221 agccccgcgc tcttgccctc tcgccccctt tgccgtatta gccccgcctt tcccgaactg
     2281 ccgggccaaa ggcatgcctt ggcggttcat gatgcgtatc cagaggacag ggtttcggcc
     2341 ctgagcaaaa tgcccaaact gaagaccaag tcaggcgcca agaagcgctt caaactgacg
     2401 gccaccggca agctgaaggc cggcgtcgcc ggcaagcgcc accggctgat cagccacaac
     2461 ggcaagtaca tccgccagca gcgcggcacg aaggtcatga gcgcttcgga ttcgaagacg
     2521 atcaagacct acctgcccta cggcctgtaa gaaggagcgc tgaacaatgg ctcgcgtcaa
     2581 acggggcgtc accgcccacg ccaagcacaa gaaggttctt gaacaggcca agggtttcta
     2641 cggccgtcgc aagaacacca tccgcacggc caaggccgct gtcgacaagg ccggccagta
     2701 cgcctatcgc gaccgcaagg tccgcaagcg cagcttccgc agcctgtgga tccagcgcat
     2761 caacgccgcc gcgcgtctgg aaggcttcac ctactcgcag tttatccacg gccttgacca
     2821 ggccggggtc gagatcgacc gcaaggttct tgcggacatc gccggccagg atccgaccgc
     2881 gttcaaggcc atcgccgaca aggttcgcgc cgcgctggct tgaagtcttg ggacatagtc
     2941 cctggaactt gaaaggccct ccggtgtgag ccggggggcc ttttgcttgg cgcgggtcgt
     3001 acgtggttcg acacgcgctc cgcgctgctc accatgacgt ggactgtgaa ggtcacccct
     3061 caacgtcatc ctgagcaggc ccggagggcc gtgtcgaagg acgcaacaag catccacgcc
     3121 ccatttcgtc gccttcccga atgctctaga ccgaaccccg catgaccgat ctcaccaccc
     3181 tcgaaaccga cctcacggcg cagatcgccg ccgcgccgga tgtcgccgcg ctggaggctg
     3241 ttcgtgtcgc cgcgctgggc aagtccggtt cgatctcggg cctgctcaag accctgggcg
     3301 ccatgagccc cgatgagcgc cgcgaggccg ggccggcgat caatggcctg cgcgaccgcg
     3361 tcgccgccgc catcgccggg cgcaaggccg ccctcgaagc cgccgagctc gacgcccggc
     3421 tggccgccga gacgctcgac ctgaccctgc ccgccccgcc ccgccgccgg ggcacggtgc
     3481 atccgacgat gcaggtcatg gacgagatga ccgccatctt cgccgagatg ggcttcgctg
     3541 tcgccgaagg gcccgacatc gagaccgact tcaacaactt caccgccctg aacttccccg
     3601 agaagcatcc ggcgcgggag atgcacgaca ccttcttctt caagccgggt cccgacggcg
     3661 agcgcaagct gctgcgcacc cacaccagcc cggtgcaggt gcgggccatg cagcgcacca
     3721 accagccgcc gcccgcgccc tggatcgcca ccgccatcga gccgccgatc cgcatcgtcg
     3781 tgccgggccg cacctatcgc tgcgacagcg atgcgaccca ttcgccgatg ttccaccaga
     3841 tggaaggcct ggtgatcgac aagggcatcc acatgggcca cctgaagtgg accctggaga
     3901 ccttcatcgc ccgcttcttc gaggtcgagg gcgtggtcag ccggttccgg ccgcaccact
     3961 tcccgttcac cgagccgagc gccgaaatgg acatcggctg cgaccggtcg ggcggcgaga
     4021 tcaagatcgg cacgggcagc gactggctcg agatcctcgg ctgcggcatg gtccatccga
     4081 acgtgctgcg cttctgcggc atcgatccgg acgtctatca gggcttcgcc ttcggcctcg
     4141 gcatcgaccg cctgggcatg ctgaaatacg gcatgcccga tctgagagac atgttcgagg
     4201 gcgacacccg ctggctgggc cactacggct tctcggcctt cgccgccccc aaccccgcga
     4261 caggactgag ctgatggcct cccacctcga gaacattccc tggaccctgg ccaaggacct
     4321 caacgccaag gccttcgaca aggaagtcgt catcctgtcc gatctctatc gggcggcgat
     4381 ccagaacggc cagtcgttca tcgagaaccg cagcttcacc gactgtcggc tggaaggtcc
     4441 ggcggtggtg gcggtggtcg gcggggtcag cttcgacagc accgacttcg gctacaccgg
     4501 cggcgacgtc ggccggatcg tctggcgcac ggcctcgacg accggcgtgg tcggggcgat
     4561 tccgttccgc aattgccagt tcaagggctg ttcgttcttc gccaccggct tcaccggccc
     4621 ggaagccttc ctgcagcaga tcctcgccct gaagaccgtc ccatgaagtt caccctctcc
     4681 tggctccggg agcacctcga caccgaggcc acgacggcgc gggtggtcga ggccatgacc
     4741 atggccggcc tcgaggtcga gcacgtcatc gacccggcga cgaagctcgc ccccttcacc
     4801 gtggcgagga tcgccgaggc cgtgcagcac cccaacgccg accggctgcg ggtctgccag
     4861 gtcgacaccg tcgacggccg caaggagatc gtctgcggcg cgcccaacgc ccgggccggc
     4921 ctgacgacca tctacgcccc gatcggcagc tatgtgccgg gcctcggcgt gaccctggtc
     4981 gagaagcccg tgcgcggcgt ggtctccaac ggcatgctct gctcggccgc cgagatggag
     5041 accgccgagg agtccgacgg catcctcgag ctcgacgaca gcctgccggt cggaaccccg
     5101 gcggcgcagg cgctcggcct ggaagccgtg atcgacttcg aggtcacgcc caaccgcccc
     5161 gactggctcg gcgtcgcggg catcgcgcgc gacctgtccg ccgccggcct tggcccgctg
     5221 cgggacctgg ccgtgaaacc cgtgcccggc cagttcccct cgccgatcgc cgtcagggtc
     5281 gacggccagg cctgccgggt gttcgccggc cggctgatcc ggggcgtgaa gaacggcccc
     5341 tcccccgcct ggctgcaggc gcgactaaag gccatcggcc ttcgtccgat caacgccctg
     5401 gtcgatgtca ccaacctgat cgcctacgac cgcgcccggc cgttgcacgt ctatgacgcg
     5461 gccaggctgg tcgggacgac gatcgaggcg cggctgggcc accacagcga ccacggcgag
     5521 gagcggctga tcgcgctgga cggcaagacc tacgacctgg gcccggagat gagcgtcatc
     5581 gccgacgccg acggcgagcg gccgatcggc ctgggcgggg tgatgggcgg cgagagcacg
     5641 ggctgttcgg acgacaccac cgaggtgttc gtcgagagcg cctggttcga cccgatcgtc
     5701 acggcccaga ccggccgcgc caccggcatc acctccgacg cccagtaccg cttcgcgcgt
     5761 ggcgtcgacc ccggcttcgt cgttgacggg ctggagctgg ccacgcggct gatcctcgag
     5821 ctgtgcggcg gcgagccctc ggacatcgtg gtcgccggcg agccgccggc gccgcccgcg
     5881 ccggtggcct tcgaccccga ctatgtgcgc cagctgtcgg gcctgaccat cccggccgac
     5941 cggtcggcgc agattctcgg gaccctcggc ttcgccatcg accgggccgg ccccgtctgg
     6001 accgtcaccc cgccgacctg gcggcgcgac gtcgagggca aggccgacct ggtcgaggag
     6061 gtgatgcgca tccacggcgt cgacgagatc
//
LOCUS       DAORNX010000141         6088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9619_length_6088_cov_7.909000, whole genome shotgun sequence.
ACCESSION   DAORNX010000141 DAORNX010000000
VERSION     DAORNX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_6088_cov_7.909000"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLO65_07650"
     CDS             complement(<1..653)
                     /locus_tag="PLO65_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28157.1"
                     /translation="MSTDLFSLKGRTALVTGGSRGIGRMIATGFIAAGARVYISSRKA
                     GACDATAAELSAGGGVCISLPQDVSTVEGCGLLAQAYGKYETSLDILVNNAGAAWLAE
                     FDSFPEAGWDKVVDLNLKSPFFLTQALAPALRASATHERPAKVINIASIDGIRPNPQE
                     TYSYQASKAGLIHLTRRMAGRLIEDNIVVTAIAPGAFASDMNITARDQSEAVAARIPS
                     "
     gene            complement(681..1004)
                     /locus_tag="PLO65_07655"
     CDS             complement(681..1004)
                     /locus_tag="PLO65_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HRD28158.1"
                     /translation="MSQIDPATGRYASFEAFYPFYIHEHSNRTCRRIHVVGTGLVIAA
                     FVTALATRNPWWFAAMPLVGYGFAWVGHFVFEKNTPATFKYPLWSLMGDFRLFFETVT
                     GKRRW"
     gene            1124..1333
                     /locus_tag="PLO65_07660"
     CDS             1124..1333
                     /locus_tag="PLO65_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRD28159.1"
                     /translation="MQALPIAAAGLNDAAARFDASARRTAAAPLDDLVRETVERAEAK
                     AAVGANAAVIRTADEMTGTVLDMFA"
     gene            complement(1558..2016)
                     /locus_tag="PLO65_07665"
     CDS             complement(1558..2016)
                     /locus_tag="PLO65_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD28160.1"
                     /translation="MPSALTHATFCVERVYDAAPARVFHAFTDREARMRWFFKTDSWT
                     LHDHSGGELGVGRTESSRFSPPGADVIITNDSIYLDVAPNERVIFAYAMTLGGAPLSS
                     SLSTVEFRPEGKGTRIVFTEQGVYLDGNVDGRIEGTEGLLTTLGEDLARG"
     gene            complement(2029..2370)
                     /locus_tag="PLO65_07670"
     CDS             complement(2029..2370)
                     /locus_tag="PLO65_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD28161.1"
                     /translation="MLQQSPSLDPVFQALADPARRSMLERLTLGPASVSQLAEPLPMS
                     LAAVGQHIAVLEAAGLVKSQKVGRVRTCTLDTTVLTQAEKWINDRRVAVERQLDRLGE
                     YLAATKPADEA"
     gene            2500..2847
                     /locus_tag="PLO65_07675"
     CDS             2500..2847
                     /locus_tag="PLO65_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRD28162.1"
                     /translation="MAYIDANVLAVPTARKDAYIAFAREVDPLFRECGALSVVDAWGD
                     DVPEGKLTDYRRAVQATADETVVLGWITWPDKATRDAGWEKVMQDPRMQTFNPPFDGK
                     RMIFGGFEVVVEV"
     gene            2913..3206
                     /locus_tag="PLO65_07680"
     CDS             2913..3206
                     /locus_tag="PLO65_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD28163.1"
                     /translation="MEWEVRQSPAFKVWVLSLRDRTARAIILRRIDRIAVGNFGDVKA
                     MGGGVSELRVNHGPGYRIYFVRRGETLVVILCGGDKGSQSKDVARAKALAQEI"
     gene            3208..3414
                     /locus_tag="PLO65_07685"
     CDS             3208..3414
                     /locus_tag="PLO65_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28164.1"
                     /translation="METLPFDPAEYLDSEEAIAAYLADAQQDGPAAVSRAAVVVARAR
                     QVIEAERLRRGVGETAAPPFKSGE"
     gene            complement(3457..5409)
                     /gene="rpoD"
                     /locus_tag="PLO65_07690"
     CDS             complement(3457..5409)
                     /gene="rpoD"
                     /locus_tag="PLO65_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRD28165.1"
                     /translation="MSTTATEQTDAPEAPEKDGPLLDLTDAGVKKFIKQAKTRGYVTM
                     DELNKVLPSEEVTSEAIEDTLAMLSEMGVNVVEAEEDAVEGEGGEVVAAGETAIVTEA
                     ETKSTYDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEA
                     IMVWREELGTGRILLREVIDLEGTYGVMFPAAPVIVPTEDAEAAEPKEAVEGEEKPEG
                     EDDDDDFDDGAGPTVSAMEGELRESVMETLDAIAAEFDAFRRLQEKLVERRLKGGDLK
                     EDDRKAYEAASGAIVARLKTLKLNNNRIEALVDQLYAINRRLIALEGRLLRLADSYGI
                     SRAEFLKAYFGSELDPNWSEKVKALGVRWTKFSENDDSSIRDIRQEVAALATETGVPI
                     DDYRRIVQTVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLM
                     KAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQML
                     HEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAV
                     LPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERI
                     RQIEAKALRKLKHPSRSRKLRSFLDS"
     gene            complement(5483..>6088)
                     /locus_tag="PLO65_07695"
     CDS             complement(5483..>6088)
                     /locus_tag="PLO65_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRD28166.1"
                     /translation="QGVRTPFVPNRPWRPGGRPGQAFEAAYPTPEGKAAARRLATALD
                     PLAAALAKGALEDPHRLDDHLEAVEKAGFGDPALSALAAEIIRLRLDADHLDSGALAR
                     HLRERGFAGLLTDIDRAAAQAGAPFLKQDVTLATARSQWTHAFEVLNRLAALEAAIAS
                     AKGNLGGRSDMAALERLKAERDVLKRSVRSGSLWTDGETSP"
BASE COUNT          911 a         2011 c         2113 g         1053 t
ORIGIN      
        1 gacgggatgc gggcggcgac ggcctcggac tggtcccggg cggtgatgtt catgtcggag
       61 gcgaacgccc ccggcgcgat ggccgtgacg acgatgttgt cctcgatcag ccgtccggcc
      121 atgcgccgcg tcagatggat caggcccgcc ttgctggcct ggtagctgta ggtttcctgc
      181 gggttgggcc ggatgccgtc gatcgaggcg atgttgatca ccttggccgg ccgttcgtgc
      241 gtcgccgagg cgcgcagggc cggggccagc gcctgggtca ggaagaacgg cgacttgagg
      301 ttcaggtcca cgaccttgtc ccagcccgct tcgggaaagc tgtcgaactc ggccagccag
      361 gcggcgccgg cgttgttgac caggatgtcg aggctggttt cgtacttgcc gtaggcctgg
      421 gccagcaggc cgcagccctc gaccgtcgag acgtcctgcg gcaacgagat gcacaccccg
      481 cccccggccg acagctcggc cgccgtggcg tcgcaggcgc cggccttgcg cgaggagatg
      541 tagacccgcg cgccggcggc gatgaagccg gtcgcgatca tcctgccgat gccgcgcgag
      601 ccgccggtga ccagggccgt gcggcccttg agggagaaga ggtcggtgct catgggtcag
      661 tcctgtgagt tgggatggcg ctaccagcgc cgcttgccgg tgaccgtctc gaagaacagg
      721 cggaagtcgc ccatcaggct ccagagcggg tatttgaagg tggccggggt gttcttctcg
      781 aacacgaaat ggccgaccca ggcgaaaccg tagccgacca gcggcatggc cgcgaaccac
      841 caggggttcc gcgtcgcgag cgccgtcacg aaggccgcga tgaccaggcc cgttcccacc
      901 acatggatcc gccggcaggt ccggttcgaa tgctcgtgga tgtagaaggg gtagaaggcc
      961 tcgaacgagg cgtaccgtcc ggtcgcggga tcgatctggc tcatggggcc gaaaggtgcg
     1021 gcagggccgg tgctctggca agcggtccgt ttaccttgct tgccgacggc ccgttaagcg
     1081 cgccgggcgc aggctgtgcc gtcgccaacg ggagaaaccc gccatgcagg ccctgcccat
     1141 cgccgccgcc ggcctcaacg acgccgccgc ccgtttcgac gccagcgccc ggcgcacggc
     1201 cgccgcgccg ctcgatgacc tcgtccgcga aacggtcgag cgggccgagg ccaaggcggc
     1261 ggtcggggcc aatgccgcgg tcatccgcac cgccgacgag atgaccggga cggtgctgga
     1321 catgttcgcc tgattcaggc cgaccaggtt ctctcgcggc ggagatcgtc ctgatggctt
     1381 gatgagcatg cttgctccgg cgagaccttc gtcaaggacg ggcccgcagg gcccgccgct
     1441 gcgcggccgc gaagcggtcc ttgaccaagg tcgccgtccg gagcggacca ttccatcaag
     1501 gccttcaggg cgcatcagta tccgggcgta tggagtgtga gcacgcccgc gccaacgtca
     1561 cccccgcgcc aggtcctcgc ccagcgtcgt cagcaggccc tcggtcccct cgatccggcc
     1621 gtcgacgttg ccgtccagat agacgccctg ctcggtgaac acgatccgcg tgcccttgcc
     1681 ctccggcctg aactccaccg tcgacaggga cgaggacagc ggcgccccgc ccagggtcat
     1741 ggcgtaggcg aagatcaccc gctcgttggg cgcgacatcg agatagatgc tgtcgttggt
     1801 gatgatcaca tccgctcccg gcggcgagaa ccggctcgac tcggtcctgc cgacgcccag
     1861 ctcaccgcca ctgtggtcgt gcagcgtcca gctgtcggtc ttgaagaacc agcgcatccg
     1921 cgcctcgcgg tcggtgaagg cgtggaacac gcgggccggc gcggcgtcat agacccgctc
     1981 gacgcagaag gtggcgtggg tcagggcgga gggcatggcg gggtcctttc aggcttcgtc
     2041 ggccggcttc gtggcggcga gatactcgcc cagccggtcg agctggcgct cgacggcgac
     2101 ccggcggtcg ttgatccact tctcggcctg ggtcagcacc gtggtgtcga gggtgcaggt
     2161 ccgcacccgc ccgaccttct ggctcttcac cagcccggcc gcctcgagca cggcgatatg
     2221 ctggccgacg gcggcgaggc tcatcggcag gggctccgcc agctggctga ccgacgccgg
     2281 cccgagggtc agccgctcga gcatcgaccg ccgcgccgga tcggcgagcg cctggaagac
     2341 ggggtcgagc gagggtgatt gctgaagcat atgctttagt atttcggcgc gatgatcggc
     2401 gcaagcaaaa agtaaagctg tcgcttgagt gacgactgtc ggccgggact cggtcgcgat
     2461 gcgcgcgtta tcgtcacggc gaacacaagg gagaccgcca tggcctacat cgacgccaac
     2521 gttctcgccg tgccgacggc caggaaggac gcctacatcg ccttcgcccg cgaggtggat
     2581 ccgctgttca gggaatgtgg cgccctgagc gtggtcgacg cctggggtga tgacgtgccc
     2641 gagggcaaac tcaccgacta ccgccgcgcg gtccaggcca cggccgacga gaccgtggtg
     2701 ctgggctgga tcacctggcc cgacaaggcg acccgcgacg ccggctggga aaaggtcatg
     2761 caggacccgc gcatgcagac cttcaacccg ccgttcgacg gcaagcggat gatcttcggc
     2821 gggttcgagg tggtggtgga ggtttaacgt cgcgcggacg cattaagggc aaatgttgtt
     2881 gacacaccct tggattgcgc tttccgtgtc gcatggaatg ggaggtccgg caaagcccgg
     2941 cgttcaaggt ttgggtgctg tcactccggg accgtaccgc tcgagcgatt atcctccgca
     3001 ggattgatcg tatcgccgtt ggcaatttcg gcgacgtcaa agccatgggg ggcggcgtaa
     3061 gcgaactgcg ggtcaaccac ggcccgggct accggatcta cttcgttcgg cgcggcgaaa
     3121 ccctggtcgt gatattgtgc ggcggcgaca agggatccca gtcgaaggac gtcgctcgcg
     3181 ccaaagcgct ggcgcaggag atttagcgtg gaaacactgc ctttcgatcc ggccgaatac
     3241 cttgattcag aagaggcgat cgccgcctac ctcgccgacg cccagcaaga tggccctgcg
     3301 gcggtctcac gggcggccgt ggtcgtcgcc cgggcccgcc aggtgatcga ggcggagcgg
     3361 ttgcgccgag gcgtcggcga gacagccgcg cctccgttca agagcggcga atagcgcaac
     3421 gaaaaagggc ggctcccgcg agccgcccct tccgtcctaa ctgtccagga agctgcgcag
     3481 cttccggctc cggctcgggt gcttgagctt gcgcagggcc ttggcctcga tctggcggat
     3541 gcgttcgcgg gtgacgctga actgctggcc gacctcttcg agggtgtggt cggtgttcat
     3601 gccgatgccg aagcgcatgc gcagcacgcg ttcctcgcgc ggcgtcaggg acgccagcac
     3661 gcgggtggtg gtctcgcgca ggttcgactg gatcgccgcg tcgatcggca ggacggcgtt
     3721 cttgtcctcg atgaagtcgc ccaggtggct gtcttcctcg tcgccgatgg gcgtctcgag
     3781 gctgatcggc tccttggcga tcttgaggac cttgcgcacc ttctccagcg gcatggccag
     3841 cttctctgcc agctcctcgg gggtcggctc gcggccgatc tcgtgcagca tctggcggct
     3901 ggtccggacg atcttgttga tcgtctcgat catgtgcacc gggatgcgga tggtccgcgc
     3961 ctggtcggcg atcgagcggg tgatggcctg gcggatccac caggtggcgt aggtcgagaa
     4021 cttgtagccg cggcgatact cgaacttgtc gaccgccttc atcaggccga tgttgccttc
     4081 ctggatcagg tcgaggaact gcaggccgcg gttggtgtac ttcttggcga tggagatcac
     4141 caggcgcagg ttggcctcga ccatttcctt cttggcctga cgcgcctcgc gctcgccctt
     4201 ctgcacggtc tggacgatgc ggcggtagtc gtcgatcggc acgccggtct cggtggccag
     4261 ggcggccact tcctgacgga tgtcgcggat cgagctgtcg tcgttctcgc tgaacttggt
     4321 ccagcgcacg cccagcgcct tgaccttttc cgaccagttg ggatcgagct cggagccgaa
     4381 ataggccttg aggaactcgg cgcggctgat gccgtagctg tcggccaggc gcagcagccg
     4441 gccttccagc gcgatcaggc ggcggttgat cgcatagagc tggtcgacca gagcctcgat
     4501 gcggttgttg ttcagcttga gcgtcttcag gcgcgcgacg atggcgccgg aggcggcctc
     4561 gtaggccttg cggtcgtcct ccttgaggtc gccgcccttg agccggcgct cgaccagctt
     4621 ttcctgcagg cgacggaagg cgtcgaactc ggcggcgatg gcgtcgagcg tctccatgac
     4681 gctctcgcgc aactcgcctt ccatggcgct gacggtcgga ccggcgccgt cgtcgaagtc
     4741 gtcgtcgtcg tcctcgccct ccggcttctc ctcgccctcg acggcttcct tgggttcggc
     4801 cgcctcggcg tcctccgtcg gcacgatcac cggcgcggcc gggaacatca cgccgtaggt
     4861 gccttccagg tcgatgacct cacgcagcag gatccggccg gtgccgagct cctcgcgcca
     4921 gaccatgatc gcctcgaagg tcagggcgct ctcgcacagg ccgcggatca tcgtgtcgcg
     4981 accggcctcg atgcgcttgg cgatggcgat ctcgccctcg cgcgacagca gttccacgct
     5041 gcccatttcg cgcaggtaca tgcgcaccgg gtcatcggtg cgatcgtagg tcgacttggt
     5101 ctcggcctcg gtgacgatcg ccgtctcgcc ggccgcgacc acctcgccgc cctcgccctc
     5161 gaccgcgtcc tcctcggcct cgacgacgtt gacgcccatc tcgctgagca tggcgagggt
     5221 gtcctcgatg gcctcggagg tcacctcctc gctcggcagc accttgttca gctcgtccat
     5281 ggtgacgtag ccgcgggtct tggcctgctt gatgaatttc ttgaccccgg catcggtgag
     5341 gtcgaggagc gggccgtcct tctcgggggc ctcgggggcg tcggtctgct cggtggcggt
     5401 cgtgctcatc gcgatctccg gcgggccgcc cccggatcgg gcgggacgcc tcatgaaaca
     5461 gtcagggacg caacgacgcg tctcagggag aggtttcacc gtccgtccac agggatcccg
     5521 agcgaaccga tcgcttgagg acatcccgct cggccttcag ccgctccagc gccgccatgt
     5581 cggaacgccc cccaaggttc cccttggcgg acgcaatagc agcctcgagc gcagccagtc
     5641 ggttcagaac ctcaaaagca tgcgtccact gggacctggc ggtggcgagg gtgacatcct
     5701 gtttcaggaa gggcgcgcct gcttgagcag cggcccggtc gatgtcagtc agcagcccgg
     5761 caaatccacg ttcccgcaga tggcgtgcga gggccccgga gtcaaggtga tcggcgtcaa
     5821 gacgcaggcg gattatttcc gccgcgagcg ccgaaagggc cggatcgccg aagccggctt
     5881 tctcgacagc ctccagatga tcgtccagcc ggtgtgggtc ctcaagggcc cccttggcca
     5941 gggccgcggc cagcgggtcg agcgccgtgg ccagccgccg ggcggccgcc ttgccctccg
     6001 gcgtgggata ggcggcctcg aaggcctgac cggggcggcc gccgggcctc cacggccggt
     6061 tggggacaaa cggcgtgcgg acgccctg
//
LOCUS       DAORNX010000142         6069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9652_length_6069_cov_6.925840, whole genome shotgun sequence.
ACCESSION   DAORNX010000142 DAORNX010000000
VERSION     DAORNX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9652_length_6069_cov_6.925840"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLO65_07700"
     CDS             <1..628
                     /locus_tag="PLO65_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28167.1"
                     /translation="EPEPEPRPEAAAPSYTPPYPQPTPIVVGSSLALASGDPGFSLTP
                     APEVLAERVTDDALRADLPEPANEAGPGLFDVALSEMVLEGAPARPLVSEDYVRRLVR
                     EDEMAAMAEFDPAFAETPTEPARIAGLPLMIMAVSGMALFAGGVAVGFTAADGDGLLS
                     IRNLGWLLAVGGIGLTGGAIYVALNKFLGAPLDDEDEEVTEAESEPQA"
     gene            813..1976
                     /locus_tag="PLO65_07705"
     CDS             813..1976
                     /locus_tag="PLO65_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRD28168.1"
                     /translation="MTQTGPAMDFGLGETADAIRETTARFAADRIAPIAADIDRDNAF
                     PRGLWPQMGELGLHGITVEEEFGGLGLGYLEHVVAMEEVSRASASVGLSYGAHSNLCV
                     NQIRRWATPEQKRRYLPKLISGEHVGALAMSEAGSGSDVVSMKLRADRRGDRFVLNGT
                     KFWITNAPHADTLVVYAKSAPEEGSRGITAFLIEKGMKGFSVSRKLDKMGMRGSDTAE
                     LVFEDCEVPEENIMGPFNGGVGVLMSGLDYERAVLAAGPLGIMQACLDVVLPYVRDRK
                     QFGKAIGSFQLMQGKVADMYVALNSARAYVYAVARACDAGKTTRFDAAGAILMASENA
                     VRVANEAVQALGGAGYTREWPVERYVRDAKLYDIGAGTNEIRRFLIGRELVGG"
     gene            2163..2345
                     /locus_tag="PLO65_07710"
     CDS             2163..2345
                     /locus_tag="PLO65_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HRD28169.1"
                     /translation="MPAKSAAQQKAAGAALSAKRGDTPKSELRGASRSMMSMSEKELE
                     EFAHTRRKGKPEHVKR"
     gene            complement(2347..3489)
                     /locus_tag="PLO65_07715"
     CDS             complement(2347..3489)
                     /locus_tag="PLO65_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD28170.1"
                     /translation="MTLQATRRGLIAGAGAALAAPGLGLAAAADDGPLPEDGYYEVIE
                     QQNSDRLWVIRQARPFHLQPIGNVTVIDQVDGLVMVDCGGSPGSGRRIVNLIRRVSAK
                     PVKAVILTHWHGDHPLGLAEILKAWPGARTIATAATRAHLSEPATMNTPATFEAKANA
                     ALVSRYRGFADYCRQQSAAATTDADRAGWAAGGRLFNQYARDMDGAVTIAPREGFTDE
                     LVIDDRDQPVVATFLGRANTDGDAIVRVPRQGLLITGDLVVAPVPFAYGSYPSDWLAA
                     LKTLINMDWTRLVPGHGAPMNSSEHLLRVGALLEAAIAGAKGLPPGTTLADARAKLDF
                     SARGRWFTGGDPWLDRWFKSYWTDPVIASAWKEANGVPIVQSLGGD"
     gene            complement(3861..4316)
                     /locus_tag="PLO65_07720"
     CDS             complement(3861..4316)
                     /locus_tag="PLO65_07720"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HRD28171.1"
                     /translation="MKTIRILALIGLLGLAAAPALAREETTTILPGWWESTNKATMVI
                     SKTEVERRCITPADVDKILSGRINRHYTCTYPVKRVGDGRMYFQGTCVDKRGRKVNIT
                     ANGTYSATAFQLSADLSGKYGGIPLAASMSTSARRVGDTCLGKPLKIKG"
     gene            4410..5183
                     /locus_tag="PLO65_07725"
     CDS             4410..5183
                     /locus_tag="PLO65_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD28172.1"
                     /translation="MPSWDPDIYERYKAYRDRPALDLMVQIPDRRFREIWDLGCGTGE
                     HAALLALRHPGARVHGLDSSPDMLRQAKNRLSEVDWVLSDIAAFAPAAPPDLIFTNAA
                     LQWLPDHAALIPRLAGLLAPGGVFACQMPMAWETEHHRILREVAEEGPWADLTRGARR
                     INPTPPLDAYYDWLSGVCGQADLWTTTYLHALEGEDPVVDWMRGTALRPYLDVLAAEP
                     ALLEAFLDTFRARIAEAFPPRADGVTLFPFPRLFMVAVR"
     gene            complement(5191..>6069)
                     /locus_tag="PLO65_07730"
     CDS             complement(5191..>6069)
                     /locus_tag="PLO65_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD28173.1"
                     /translation="ARMARVLTGRSVGLVLSGGGARAYAHIGAIQALRERGVPIDFVG
                     GASMGAIVAAGVAMGWDEAEMDRRIRDAFVDSSPLDDVAFPLIAMTHGEKVRGRLREH
                     FGDSQIADLWLPFFCVSSNLTTGAYHLHRRGPLRHALRASIALPGVMPPAIDNNNVLV
                     DGAVMKNFPSDVMRATQLGPIVGVDVTRGRSITADDVARPSSVWRWLLSGDWRKGPPI
                     VALLMRAATVSTGRDLLAAREATDLLILPKVDGVDIRDWKAFEPAVDAGYRAAVAALD
                     ALERPVTEMRKARAKG"
BASE COUNT          930 a         2129 c         2104 g          906 t
ORIGIN      
        1 cgagcccgag cccgagcccc ggccggaggc cgcggccccg tcgtacacgc cgccctatcc
       61 gcagccgacg ccgatcgtgg tcggctcgag cctggccctg gccagtggcg accccggttt
      121 ctccctgacc ccggctcccg aggtgctcgc cgagcgggtg acggacgacg cgctgcgcgc
      181 cgacctgccc gagccggcca acgaagccgg ccccggcctg ttcgatgtcg cgctgtcgga
      241 gatggtgctc gaaggcgcgc ccgcccggcc gctggtcagc gaagactatg ttcgccggct
      301 cgtgcgcgag gacgagatgg cggcgatggc cgagttcgat cccgccttcg ccgagacccc
      361 gaccgagccg gcccgtatcg ccggcctgcc gttgatgatc atggcggtca gcggcatggc
      421 cctgttcgcg ggcggcgtcg cggtcggctt caccgccgcc gacggcgacg gcctgctgag
      481 catccgcaac ctgggctggc tgctggcggt gggcggcatt ggactgacgg gtggcgcgat
      541 ctatgtggcc ctcaacaaat tcctcggcgc cccgctggac gacgaggacg aggaagtgac
      601 cgaggcggag tcggagcctc aagcgtaacc cgcgtagcgt atgggaaccc gcgtacagta
      661 aggggatagc gttggcgccc cgcgagcctg ctcgcggggc gtcgtgcgtt ttcagcacgg
      721 ggacgtgcgc ttcagtgcgt gtccccttcc tgcgcgaggg ggcacgcact gaagtgcatg
      781 tccccgttca ttcatcgtgc tatttccccg ccatgaccca aaccggaccc gccatggatt
      841 tcggcctcgg cgaaaccgcc gacgccatcc gcgaaaccac cgcccgtttc gccgccgacc
      901 gcatcgcccc gatcgccgcg gacatcgacc gcgacaacgc ttttccgcgc ggactctggc
      961 cgcagatggg cgaactcggc ctgcacggca tcacggtcga ggaggagttc ggcggcctcg
     1021 gcctcggcta cctagagcac gtcgtggcga tggaggaggt gtcccgcgcc tcggcctcgg
     1081 tcggtctcag ctacggcgcc cactccaatc tctgcgtcaa ccagatccgc cgctgggcca
     1141 cgcccgagca gaagcgccgc tacctgccga aactgatcag tggcgagcat gtcggggccc
     1201 tggccatgag cgaggccggc tcgggctcgg acgtggtctc gatgaagttg cgcgccgatc
     1261 ggcgcggcga ccgcttcgtg ctcaacggga ccaagttctg gatcaccaac gcgccgcacg
     1321 ccgacaccct ggtggtctac gccaagtcgg cgccggagga gggcagccgc gggatcaccg
     1381 ccttcctgat cgagaagggc atgaagggct tcagcgtcag caggaagctc gacaagatgg
     1441 gcatgcgcgg cagcgacacg gccgagctgg tgttcgagga ctgcgaagtc ccggaagaga
     1501 acatcatggg cccgttcaac ggcggcgtcg gggtgctgat gagcggcctc gactatgaac
     1561 gcgccgtgct ggccgccggg ccgctgggca tcatgcaggc ctgcctcgac gtggtcctgc
     1621 cctatgtccg cgaccgcaag cagttcggca aggcgatcgg cagcttccag ctgatgcagg
     1681 gcaaggtcgc cgacatgtat gtcgcgctca actcggcccg cgcctacgtc tatgcggtgg
     1741 ccagggcctg cgatgccggc aagaccaccc ggttcgacgc cgccggcgcc atcctgatgg
     1801 ccagcgagaa cgccgtccgc gtggccaacg aggccgtcca ggccctcggc ggcgccggct
     1861 acacccgcga atggccggtc gaacgctatg tgcgcgacgc caagctctac gacatcggcg
     1921 cgggcaccaa cgagatcagg cggttcctga tcggcaggga gctggtcggg gggtagcccc
     1981 gactgtcgtc ccggacggcc cgcagggacg atccgggacc caggtcaagt cccggcagcc
     2041 ctgcttgaag ccgaatcggg gtccgggatc gcctccggcg tccgggatga cagccggagg
     2101 gaacgccatg ggcagactgt cgttgagttg ttcacggtcg gggcctgtga aggagccaga
     2161 ccatgcccgc caagtccgcc gcccagcaga aggccgccgg ggccgccctg tccgccaagc
     2221 ggggtgacac cccgaagtcc gagctcaggg gcgccagcag gtcgatgatg tcgatgagcg
     2281 agaaagagct cgaagagttc gcccacacca gacgcaaggg caagcccgag cacgtcaagc
     2341 ggtaggtcag tcgccgccca ggctctggac gatcggcacg ccgttggcct ccttccaggc
     2401 cgaggcgatg accggatcgg tccagtagct cttgaaccag cggtccagcc agggatcgcc
     2461 gccggtgaac caccgtcccc gtgcggaaaa atcgagcttc gcgcgggcgt cggccagggt
     2521 cgtcccgggc ggcaggcctt tggccccggc gatggccgct tccagcagcg cccccaccct
     2581 cagcaggtgc tcgctgctgt tcatcggcgc cccgtggccg gggacaagcc tggtccagtc
     2641 catgttgatc agggtcttga gcgccgcgag ccagtccgac gggtagctgc cataggcgaa
     2701 cggcacgggc gcgacgacca ggtcgccggt gatcagcagg ccctgccgcg gaacccgcac
     2761 gatcgcgtcg ccatcggtgt tggcccgacc caagaaggtc gccaccacag gctggtcgcg
     2821 gtcatcgatc accagctcgt cggtgaatcc ctcccggggg gcaatggtca ccgcgccgtc
     2881 catgtcgcgg gcgtactggt tgaacagccg cccgcccgcg gcccatccgg cccgatcggc
     2941 gtcggtggtc gcggcggcgg actgctggcg gcaatagtcg gcgaatcccc ggtagcgcga
     3001 gacgagggcg gcattggcct tcgcctcgaa ggtcgccggg gtgttcatgg tcgccggctc
     3061 cgacaggtgg gcgcgggtcg ccgccgtcgc gatggtccgc gcccctggcc aggccttgag
     3121 aatctcggcc agacccagcg gatggtcgcc atgccagtga gtcaggatca ccgccttgac
     3181 cggtttggcg ctgacgcggc ggatcagatt gacgatccgc cgtccggatc ccggcgagcc
     3241 gccgcagtcg accatcacca gcccgtcgac ctggtcgatg accgtgacat tgccgatcgg
     3301 ctggaggtgg aagggcctgg cctggcggat gacccagagc cggtcggagt tctgctgttc
     3361 gatgacctcg tagtagccgt cttcgggcag ggggccgtca tccgccgcgg cggcgaggcc
     3421 cagtcccggc gcggcgagcg ccgcgcccgc acccgcgatc aacccgcgtc tcgtcgcctg
     3481 caaggtcatg accgatcctc cccggggccc atgtcagcac gacaaccggg ccggatcgaa
     3541 tgaactcgcg tccacgattt gcccagccag acgcgcccgc ggacacgccc cggaacggac
     3601 ttccgggctc cagacatgcg aaaggccgcc ggggcgcccc ggcggccttt ccatcgtcat
     3661 gccgcggtcg gcggcgaaac gctaggcgct gatcaggcgc tgacctccgc cgaaacggat
     3721 ttcagggtca cgctctgcga ccgcgggttc atacgatgag acaccggatc acctcctttc
     3781 agctgttgaa acaggactca cactatgtgg ggcgtcttaa cgagtcgcca accctccgca
     3841 cgcgaggcgc gacaaattga tcagcccttg atcttcaacg gcttgcccag gcaggtgtcg
     3901 ccgacacgcc gggcgctcgt gctcatgctg gccgccagcg gaatgccgcc gtacttgccc
     3961 gacaggtcgg ccgacagctg gaaggcggtc gccgaatagg tgccgttggc tgtgatgttg
     4021 accttgcgcc cgcgcttgtc gacgcaggtg ccctggaaat acatccggcc gtcgcccacc
     4081 cgcttgaccg gataggtgca ggtgtagtgg cggttgatcc ggcccgagag gatcttgtcc
     4141 acgtcggccg gggtgatgca gcggcgctcg acctcggtct tgctgatgac catggtcgcc
     4201 ttgttggtgc tttcccacca gccgggcagg atggtggtgg tctcctcgcg cgccagcgcg
     4261 ggcgcggcgg ccaggccgag caggccgatc agggccagga tgcggatcgt cttcatgggt
     4321 ccagtctcgg gaatgagcgt ggcgtctttc tgacctaacc cgaatgaacg ggcgatgacc
     4381 accgcgtgca accggtgtag ggcaggatca tgcccagttg ggaccccgac atctacgagc
     4441 gctacaaggc gtaccgcgac cgacccgcgc tcgacctgat ggtgcagatt ccggaccgcc
     4501 gtttccgcga gatctgggac ctcggctgcg ggacgggcga gcacgcggcc ctgctggccc
     4561 tgcgccatcc gggggccagg gtgcatggcc tcgattcgag cccggacatg ctgcgccagg
     4621 cgaagaaccg cctgagcgag gtcgactggg tgctgtcgga catcgcggcc ttcgccccgg
     4681 ccgccccgcc cgacctgatc ttcaccaacg cggccctgca gtggctgccc gaccacgcgg
     4741 ccctgattcc gcgcctggcc ggtctgctgg cgcccggcgg ggtgttcgcc tgccagatgc
     4801 cgatggcctg ggagacagag caccaccgca tactgcgcga ggtggcggag gaggggccgt
     4861 gggcggacct gacccgcggc gcccgccgga tcaacccgac cccgccgctg gacgcctact
     4921 acgactggct gagcggtgtc tgcggccagg ccgatctgtg gaccaccacc tacctccacg
     4981 ccctcgaggg cgaggatccg gtggtcgact ggatgcgcgg cacggcgctg cggccctatc
     5041 tcgacgtgct ggccgcggaa ccggcgctgc tggaagcttt cctcgacacc ttccgcgcgc
     5101 gcatcgccga agccttcccg ccgcgcgccg acggggtgac actgttccca ttcccgcgcc
     5161 tgttcatggt ggcggtgcgc tagaagagtc tcaccccttc gcgcgcgcct tgcgcatctc
     5221 ggtcaccggc cgctccagcg cgtccagcgc ggcgacggcg gcgcggtagc cggcgtccac
     5281 ggccggctcg aaggccttcc agtcgcggat gtcgaccccg tcgaccttcg gcaggatcag
     5341 caggtcggtc gcctcgcggg cggcgagcag gtcgcgcccc gtcgataccg tcgccgcgcg
     5401 catcagcagg gccacgatcg gcggcccctt gcgccagtcc cccgagagca gccagcgcca
     5461 gaccgacgag ggccgcgcga cgtcgtcggc ggtgatgctg cggccgcggg tcacgtcgac
     5521 ccccacgatc ggccccagct gcgtcgcccg catcacgtcg gacgggaagt tcttcatcac
     5581 cgcgccgtcg accagcacgt tgttgttgtc gatcgccggc ggcatcactc cgggcagggc
     5641 gatcgaggcg cggagcgcgt gccgcagcgg gccgcgccgg tgcaggtgat aggcgccggt
     5701 ggtcaggttc gaggagacgc agaagaacgg cagccacagg tcggcgatct ggctgtcgcc
     5761 gaaatgctcg cgcagccggc cgcgcacctt ctcgccgtgg gtcatggcga tcagcgggaa
     5821 ggcgacgtcg tcgagcggcg aggagtcgac gaaggcgtcg cggatgcggc ggtccatctc
     5881 ggcttcgtcc cagcccatgg ccacgccggc ggcgacgatc gcccccatcg atgcgccgcc
     5941 gacaaagtcg atcggcacgc cgcgctcgcg caaggcctgg atggcgccga tgtgggcgta
     6001 ggcccgcgcg ccgcctccgg acagcaccag ccccaccgag cggccggtca gcacccgcgc
     6061 catgcgggc
//
LOCUS       DAORNX010000143         6056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9683_length_6056_cov_7.124646, whole genome shotgun sequence.
ACCESSION   DAORNX010000143 DAORNX010000000
VERSION     DAORNX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9683_length_6056_cov_7.124646"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLO65_07735"
     CDS             <1..527
                     /locus_tag="PLO65_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRD28174.1"
                     /translation="WHMLQLLTSLPALYVCNVDEASADKGNAFSDKVAERARADNAKS
                     VVISAQIESEIALLDPAERAEFLETLGLHEPGLNRLIREAYALLGLQSYFTVGPKEAR
                     AWTIEVGCTAPQAAGVIHNDFEKGFIRAETIAYDDFVAFKGEQGARDAGRMRSEGKEY
                     IVKDGDVMHFRFNV"
     gene            573..959
                     /locus_tag="PLO65_07740"
     CDS             573..959
                     /locus_tag="PLO65_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28175.1"
                     /translation="MTTDHADAIAAAEAVLDAFMAAFNARDLAAWEKTFNFPSVRLAS
                     NTLTIIEPGYHQPGMFDQGALSDWDHSAWERREVIHAGPDKVHFDTRFTRYRADGSVI
                     GGFDSIYVVTCEDGHWGVKARSSYAP"
     gene            959..1708
                     /locus_tag="PLO65_07745"
     CDS             959..1708
                     /locus_tag="PLO65_07745"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRD28176.1"
                     /translation="MSRRAELARMIDDARRVVVFTGAGISTESGIPDFRSPGGVWSKM
                     KPIQFPDFVAREDMRREAWTRVFNGSAGWVGRAPNAGHDAVARMVASGKAMSVITQNV
                     DNLHQDSGIADERIIELHGNASYATCLSCALRHELHHLRPGFVERGEIPACRSCGGIV
                     KTATISFGQPMPQDQMERAEEATLACDLFLVLGSSLVVFPAAGFPLLAKRNGAMLAIV
                     NREATDMDRYADLVLHDEIGAVMNEIVPALA"
     gene            1747..2478
                     /locus_tag="PLO65_07750"
     CDS             1747..2478
                     /locus_tag="PLO65_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28177.1"
                     /translation="MRQFNVPLFAFAALSLSLLASSPALAGESCHLEKGDSYRLTLSP
                     EITRQGSVLTVGALSAGGQQDTWGGRVALGCLKGWTVSDPGLATLSADHSTLTLAADA
                     PPGARVTLSARLAGKPVVAEITVVARDAVVLTGFWKEVGDADCPLGSPSIMELHFLPG
                     GKFELTWQPFERYVDYWGDYSFDPGTGAVTFRPTGGNHVPGDADWEGRATVGADGLLR
                     FSDLFFGSPSHGPRPPTTCIVFKAR"
     gene            2492..3175
                     /locus_tag="PLO65_07755"
     CDS             2492..3175
                     /locus_tag="PLO65_07755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD28178.1"
                     /translation="MADMITLQAAHDGFVFTALHGQPAGPRKGGVIVIQEIFGLDRFI
                     HEDVARWQARGYEVLAPSMYDRQQPGFVAEHDDAEAFQGAVKHAMANGPDNAMGDIQA
                     CIDFLRPRGPVFIVGYCYGGTMVWLAASRCTGLGAGSSYYGGQVAGMAALPLSCPVEL
                     HLGRKDSHIPCDEVKAKVQAAHPGVPVWIYENSGHGFNNDGRPESDLADAELARQRTV
                     ALFEANGAG"
     gene            3178..3852
                     /locus_tag="PLO65_07760"
     CDS             3178..3852
                     /locus_tag="PLO65_07760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD28179.1"
                     /translation="MGERIALATMGGKPFSAWRAAPGEARRGGLVVLHAIWGVTPHIR
                     AFCDGLAAQGYDVIAPSLLDGTNAFPEQDTDFSVLDERLALGETSGWGAACMDVVQAA
                     INALEGPVFLLGFCFGGTSAWLAACRCEGLKAVACFYGGRIIDFVGETPRCPTILHFG
                     ARDETIPTGDVAAITAAHPDLPAWFYDAGHAFVAPSGYHEDSARLAMLRTLQHFQRGG
                     GGKGEM"
     gene            complement(3932..4264)
                     /locus_tag="PLO65_07765"
     CDS             complement(3932..4264)
                     /locus_tag="PLO65_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28180.1"
                     /translation="MKKLILITTICAIPLLVSGAASAANNISWDDADAYCKKDRGENC
                     CAAADANGYPYVIGERQACTCGDALVANPSGAGGPAARMKKPTLAQRRAMVARNGLTW
                     VRRPVGPR"
     gene            complement(4311..4607)
                     /locus_tag="PLO65_07770"
     CDS             complement(4311..4607)
                     /locus_tag="PLO65_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28181.1"
                     /translation="MLALFVVGLSGGLAAAAPMSWDQADAFCKTSANGGPGCCAVAGD
                     DGYPKIRGDGPVCTCGELIVNGGKPLSVKERRKLARLYGERLPAARQSVPAAPR"
     gene            complement(4801..>5035)
                     /locus_tag="PLO65_07775"
     CDS             complement(4801..>5035)
                     /locus_tag="PLO65_07775"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRD28182.1"
                     /translation="LDQGEAAVEVGQILKVLQGNAAMARDLVRRLALALPVERPASPI
                     DTCLDGAIITAPDARDPAMVAKLDAVAGRVMAP"
     assembly_gap    5036..5250
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5251..5666)
                     /locus_tag="PLO65_07780"
     CDS             complement(<5251..5666)
                     /locus_tag="PLO65_07780"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRD28183.1"
                     /translation="MTGWTLGIIGGSGLYDMEGLEDRATLRLDSPFGEPSSALTTGRI
                     GATRLVFLPRHGPGHRIPPGDINARANIDVLKRAGCTDLLSLSAVGSLREDLPPGSFV
                     LVDQYIDRTVGRPSSFFGPGLTAHVSMADPVCPRLAG"
     gene            5894..>6056
                     /locus_tag="PLO65_07785"
     CDS             5894..>6056
                     /locus_tag="PLO65_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28184.1"
                     /translation="MLLAPGARAAEARCWFENGAVVIPAEIAGVAGDWLLDPSAPHTL
                     LHETRAQMEG"
BASE COUNT          867 a         2078 c         1997 g          899 t
ORIGIN      
        1 cctggcacat gctgcagctg ctgacctcgc tgccggcgct gtacgtctgc aacgtcgacg
       61 aggcctcggc cgacaagggc aacgccttct ccgacaaggt cgccgaacgg gccagggccg
      121 acaatgccaa gagcgtggtc atctcggccc agatcgagag cgagatcgcc ctgctcgatc
      181 cggccgaacg ggccgagttc ctcgagaccc tgggcctgca cgagccgggc ctcaaccgcc
      241 tgatccgcga ggcctacgcc ctgctcggcc tgcagagcta cttcacggtc ggccccaagg
      301 aagcccgcgc ctggaccatc gaggtcggct gcaccgcgcc ccaggcggcc ggggtgatcc
      361 acaacgactt cgaaaagggc ttcatccgcg ccgagaccat cgcctacgac gacttcgtcg
      421 ccttcaaggg cgaacagggc gcccgggacg ccggcaggat gcgctccgaa ggcaaggagt
      481 acatcgtcaa ggacggcgac gtgatgcact tccgcttcaa cgtctagcct ccgggccatg
      541 acgagtgggg gctggcgtat taggctcggt gcatgaccac cgatcacgcc gacgccatcg
      601 ccgccgctga agccgtgctg gacgctttca tggcggcgtt caacgcccgc gacctggccg
      661 cctgggagaa gaccttcaac ttccccagcg tgcgtctggc gtcgaacacg ctgacgatca
      721 tcgagcccgg ctatcaccag ccggggatgt tcgatcaggg cgcgctgagc gactgggacc
      781 actccgcctg ggaacggcgc gaggtgatcc acgcgggccc ggacaaggtc catttcgaca
      841 cccgcttcac ccgctaccgc gccgacggct cggtgatcgg cgggttcgat tcgatctatg
      901 tcgtgacctg cgaggacggc cactggggcg tgaaggcccg ttcgagctac gccccctgat
      961 gtcccggcgc gccgaactgg cccggatgat cgacgacgcc cggcgggtgg tggtgttcac
     1021 cggcgcgggc atctcgaccg agtccggcat ccccgacttc cgcagccccg gcggggtctg
     1081 gtcgaagatg aagccgatcc agtttcccga cttcgtcgcc cgcgaggaca tgcgccgcga
     1141 ggcctggacc cgggtgttca acggctcggc cggatgggtc ggccgcgccc cgaacgccgg
     1201 ccatgacgcc gtggcgcgga tggtcgcctc cggcaaggcg atgagcgtga tcacccagaa
     1261 cgtcgacaac ctgcaccagg acagcggcat cgccgacgag cggatcatcg agctgcacgg
     1321 caacgccagc tacgcgacct gcctcagctg cgccctgcgc cacgaactgc atcacctgcg
     1381 gcccggtttc gtcgagcgcg gcgagattcc ggcctgccgg tcctgcggcg gcatcgtcaa
     1441 gaccgcgacc atctcgttcg gccagccgat gccgcaggac cagatggagc gggccgagga
     1501 ggcgaccctg gcctgcgacc tgttcctggt gctgggctcg tcgctggtgg tgttcccggc
     1561 cgccggcttc ccgctgctgg ccaagcgcaa cggagccatg ctggcgatcg tcaaccgcga
     1621 ggcgaccgat atggaccgct acgccgacct ggtgctgcac gacgagatcg gcgcggtgat
     1681 gaacgagatc gtgccggcgc tcgcctgacg cttgtcggcg gccccgccag ctgtttcatt
     1741 cccgccatgc gccagttcaa cgttccgctt ttcgcgttcg ccgccctctc cctgtccttg
     1801 ctggcctcgt cgcccgccct cgccggggag tcctgtcatc tggagaaggg tgacagctac
     1861 cgcctgaccc tgtcgccgga aatcacccgt cagggttcgg tcctgaccgt cggcgccctg
     1921 tccgccgggg gccagcagga cacctggggc ggcagggtgg ccctgggctg cctgaagggc
     1981 tggacagtct ccgatcccgg gttggcgacc ctgtcggccg accattcgac gctgaccctc
     2041 gcggccgatg ctccgccggg ggccagggtg acgctgtcgg cccggctggc cggcaagccg
     2101 gtggtcgccg aaatcaccgt cgtcgccagg gacgccgtgg tcctgaccgg cttctggaag
     2161 gaggtcggcg acgccgactg tcccctcggc tccccctcga tcatggagct gcatttcctg
     2221 ccgggcggga agttcgaact gacctggcag cccttcgagc gctacgtcga ctactggggc
     2281 gactacagct tcgaccccgg aacgggcgcg gtcacgttca ggccgacggg gggcaaccac
     2341 gttcccgggg acgcggactg ggaaggccgg gccacggtcg gcgccgatgg cctgctgcgg
     2401 ttcagcgacc tgttcttcgg ctctccgagc catggaccga ggccgccgac aacctgcatc
     2461 gtcttcaagg cccgttagcc caggagtccc catggccgac atgatcaccc tccaggccgc
     2521 ccatgacggc ttcgtcttca ccgccctgca cggtcagccc gccgggccgc gcaagggcgg
     2581 agtgatcgtc atccaggaga tcttcggcct cgaccggttc atccacgagg acgtcgcccg
     2641 ctggcaggcc cgtggctacg aggtgctggc cccgtcgatg tacgaccgtc agcagccggg
     2701 cttcgtcgcc gagcacgacg acgccgaagc gttccagggc gccgtgaaac acgccatggc
     2761 caacggcccg gacaacgcca tgggcgacat ccaggcctgc atcgacttcc tcaggccgcg
     2821 cgggccggtg ttcatcgtcg gctactgcta cggcggcacg atggtctggc tggcggcgtc
     2881 gcgctgcacg ggtctcggcg ccggctccag ctactacggc ggccaggtgg ccggcatggc
     2941 cgccctgccc ctgtcctgcc ccgtggagct gcatctgggc cgcaaggaca gccacattcc
     3001 gtgcgacgag gtgaaggcga aggtccaggc cgcccacccc ggggtcccgg tgtggatcta
     3061 cgagaactcg ggccacggct tcaacaacga cggccggccc gagtccgacc tcgccgacgc
     3121 cgaactcgcc cgtcagcgca cggtggcgct gttcgaggcc aacggggccg gctgatcatg
     3181 ggcgagcgga tcgccctggc gacgatgggc ggcaagccct tctccgcctg gcgcgcagcg
     3241 cccggggagg cccggcgcgg cggactggtc gtgctgcacg ccatctgggg ggtgacgccc
     3301 catattcgcg ccttctgcga cggcctggcg gcccagggct atgacgtcat cgcccccagc
     3361 ctgctcgatg gaaccaacgc ctttccggaa caggacaccg atttctccgt ccttgatgag
     3421 cggctggcgc tgggcgagac ctcgggctgg ggcgcggcct gcatggatgt ggtccaggcc
     3481 gcgatcaacg ccctggaggg gccggtcttc ctgctgggtt tctgtttcgg cgggacgagc
     3541 gcctggctgg ccgcctgccg gtgcgagggg ctgaaggcgg tcgcctgctt ctacggcgga
     3601 cgcatcatcg acttcgtcgg cgagacgccc cgttgcccga cgatcctgca cttcggcgca
     3661 cgggacgaga ccatcccgac cggggatgtg gccgcgatca cagccgccca tccggacctg
     3721 cccgcctggt tctacgacgc cggccacgcc ttcgtggcgc cgagcggcta ccacgaggac
     3781 agcgccaggc tggcgatgct gcggacgttg cagcacttcc agcgcggcgg cggggggaaa
     3841 ggggagatgt gaagacgctc ccccggcctg gcaggatgcg gccgcgatgg atcgcggccg
     3901 catcccctcc ggggtccggc gcgggttgcg cctatctcgg gccgacgggt cgcctgaccc
     3961 aggtcaggcc gttgcgggcg accatcgccc tgcgctgggc cagggtgggc ttcttcatgc
     4021 gcgcggccgg gccgcccgcg cccgaaggat tggcgaccag cgcatcgccg caggtgcagg
     4081 cctgacgctc gccaatcacg taaggatacc cgttggcatc ggcagccgcg cagcagtttt
     4141 cgccacggtc cttcttgcag taggcgtccg cgtcgtccca gctgatgttg ttggcggcgg
     4201 aagccgcccc cgaaacgaga agcgggatcg cgcatatcgt ggtgatcagg atcaacttct
     4261 tcatcctggc ctccctggcc tgccggggct gtagattgtg tggcgcggca tcaccgcgga
     4321 gccgccggga ccgactgtct ggcggccggg agacgttccc catagagccg cgcgagcttc
     4381 ctgcgttcct tcacggagag gggcttgccg ccattgacga tcagctcgcc acaggtacag
     4441 acaggtccgt cgccgcggat cttcgggtag ccgtcgtcgc cggccacggc gcagcagccg
     4501 ggcccgccgt tggcggacgt cttgcaaaac gcgtccgcct gatcccacga cattggcgcc
     4561 gccgccgcca ggcctccgct gagccccaca acgaacagcg ccagcacaat tggcttcctc
     4621 atggtcgatt cctctcttga tgatgcggtc tagtgccgct tggctcccca tctgcgaaca
     4681 cgggggctga acgcaatgtg aacacagtcc gccgaccatt ggtatctgtg ttttccgacg
     4741 atcagcggcg ggcggcaggg ccgccggcga tgcggggcga cggggcggac cgcctcggag
     4801 tcagggcgcc attacccgcc ctgccacggc gtcgagcttc gccaccatcg cggggtcgcg
     4861 cgcgtccggg gccgtgatga tcgcgccgtc gaggcaggtg tcgatcggcg aggccggtcg
     4921 ctcgaccggc agggccaggg ccagccgccg gaccagatcg cgggccatgg cggcgttgcc
     4981 ctgcagcacc ttgaggatct gacccacctc gacggccgcc tcgccctgat caagannnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggccagcc gcgggcagac cggatcggcc
     5281 atcgagacat gggcggtcag gcccggcccg aagaagctcg acggccggcc gacggtgcga
     5341 tcgatatact ggtcgacgag gacgaagctg ccgggcggca ggtcctcgcg cagcgaaccc
     5401 acggccgaca gcgacagcag gtcggtgcag ccggcgcgct tcagcacgtc gatgttggcg
     5461 cgggcgttga tgtcgccggg cggaatgcgg tgtccgggcc catggcgggg caggaacacc
     5521 agacgggtcg cgccgatgcg gccggtggtc agggcgctcg acggctcccc gaaggggctg
     5581 tcgagccgca gcgtcgcgcg gtcctccagc ccctccatgt catagagccc cgagccgccg
     5641 atgatcccca gcgtccagcc cgtcatgcgc cgcccccctc tccccggatg aacatgaact
     5701 ccgacagatc gggcgggaag tccacggccg tctgccgcgg aacccgcccg gacagcacga
     5761 cgaccgccga cagccgccct tctcccgtcg aacctcgcaa cttagccatt ggtcacaacc
     5821 ggattcgcgg tccaggctgt cgtccgtgac ccatcgccct ctcctcgtcc ctgcgtttgc
     5881 cgcgggagtc gcgctgctgc tggcgcccgg cgcccgcgcg gccgaggcgc gctgctggtt
     5941 cgagaacggc gcggtggtga ttccggccga gatcgccggc gtggccgggg actggctgct
     6001 cgacccgtcg gcgcctcaca cgctgctgca cgagacgcgc gcgcagatgg agggcc
//
LOCUS       DAORNX010000144         6025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9738_length_6025_cov_7.759464, whole genome shotgun sequence.
ACCESSION   DAORNX010000144 DAORNX010000000
VERSION     DAORNX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9738_length_6025_cov_7.759464"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLO65_07790"
     CDS             complement(<1..1389)
                     /locus_tag="PLO65_07790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRD28185.1"
                     /translation="MTIALAHRDLSAFVTHDETDQGRVELLVTGARCAACMGKIERVT
                     AALPGVDAARFNLTTGKLSVRFAGRDGDPGLIIEALEKEGYPATPFDPGKAEASHDRE
                     GRALALALGVAAFGAGNAMMFTVPVWAGFFGQELDPATRQLMYWFAAAIATPCALFAG
                     QPFFRSAWASLRKGRANMDVPISIGVLLTLIVSFSETLQHGAHAYFDAAVTLLFLLLI
                     GRYLDHKLRARARSAARDLMALQAPVATRLEAGGVERAIPVGEVEVDDALVVVPGDRI
                     PVNALVEAGGSDLDNALITGETASVIVGPGSVVNAGALNLSGRLVLRVLARSEDSAVA
                     AIARLMEAGAQSRSNYVQLADKAAALYVPVVHTLAALTFAGGWALGLGPREALLRAAA
                     VLIVTCPCALGLAVPAVQIVASGRLFRKGVLVKTGAALERLAEVDHVVFDKTGVLTEG
                     RPMLLDGPPQLLA"
     gene            complement(1386..1874)
                     /locus_tag="PLO65_07795"
     CDS             complement(1386..1874)
                     /locus_tag="PLO65_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRD28186.1"
                     /translation="MTDLATTPRFTIKGWHVLAGLILFFGAVIAVDAVFISLAYRTFS
                     GEAASNPYETGLLYNRTLAQRRREAALGWSATIEEGRDDIVLVRVADRAGKPIDGLAV
                     SGALERPATIKGRRTVTFTAAGAGLYRANAAPLDGAWDLHVTIRGAGDAVFEADRRLV
                     RR"
     gene            complement(1871..3376)
                     /gene="ccoG"
                     /locus_tag="PLO65_07800"
     CDS             complement(1871..3376)
                     /gene="ccoG"
                     /locus_tag="PLO65_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRD28187.1"
                     /translation="MTVVIDRTTPPSAAKSPGPVSAAALARRKAGRGGGLYQKRAPIY
                     PKLVHGKWRTIKWAMLIATLAVYYVVPWLRWTRPGELPDQAVLVDFTGRRFYFFFIQL
                     WPQEVYFITGLLVMGALALFLVTALFGRLWCGYSCPQTVWTDLFIAVERLFEGDRNAR
                     MKLDASPMSFNKVWRKTGKHLAWLGIAFSTGGAWIFYFHDAPTLIRTFWLGQAPLTAY
                     ISCGLLTATTYGFAGAMREQVCTYMCPWPRIQGAMLDQHSLQVTYLKDRGEPRGPHKK
                     NETWEGRGDCVDCNQCVVVCPMGIDIRNGSQLECINCGLCVDACDEIMVRMGRPKGLI
                     AYDTDAAVVSRLEGRKPTYHPVRARTVYYALALTLVSAVMLWGFLQRSPVDLHVLRDR
                     NPTFVRLHDGSIRNGYTLKITNRSFGPQSFAVGLSGIRDATLKTPGESASTGLLTVTV
                     EPDEVRAVRVFVSAPARSLPATNLPVAFTIKSDVGTAEARTNFLSGEANAR"
     gene            complement(3385..4254)
                     /gene="ccoP"
                     /locus_tag="PLO65_07805"
     CDS             complement(3385..4254)
                     /gene="ccoP"
                     /locus_tag="PLO65_07805"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRD28188.1"
                     /translation="MTGDAREKDAISGVDMTGHEWDGIRELDNPLPRWWLWVFYACIA
                     FSIGYWVAMPAWPGLNTYTQGLLGKSDRREVARALEDLAVTRGAGGAKLKAAALAQIE
                     SDPQLQAYALQVGQSVFGDNCATCHGSGGGGGKGYANLRDDVWLWGGTLEDIQRTITY
                     GIRSGDPEARVSQMPAFGRDGMLQAAQIADLTEYVVALSGRPANASAVGRATPVFAEQ
                     CASCHGVAGKGDMALGAPDLTDADWLYGSDRAAISGQIQNGRGGVMPAWGHRFDPETI
                     KALAVYIHANSGQ"
     gene            complement(4251..4406)
                     /locus_tag="PLO65_07810"
     CDS             complement(4251..4406)
                     /locus_tag="PLO65_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRD28189.1"
                     /translation="MTYETVARIAQQGGLVYFALLFLGGCAYALWPKNGDEFARAARL
                     PLEEEEL"
     gene            complement(4420..5163)
                     /gene="ccoO"
                     /locus_tag="PLO65_07815"
     CDS             complement(4420..5163)
                     /gene="ccoO"
                     /locus_tag="PLO65_07815"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRD28190.1"
                     /translation="MSMWKHHGKLERHSLLLVIGIVLVVSVGGLVEIAPLFWIKNTIE
                     KVDGVRPYTPLELAGRDIYIREGCYLCHSQMIRPLRDEVERYGHYSLAAESMYDHPFQ
                     WGSKRTGPDLARVGGKYSDSWHRDHLIDPRSVVPESVMPPYAFLATKDLQTRDLQAKL
                     AAMTKVGVPYTQEQIDQTMTDLQAQADPFSSDAPALRARYGAKVVNRDFDGDPERLTE
                     MDALIAYLQMLGTLVDFSTYQAAAPANQR"
     gene            complement(5174..>6025)
                     /locus_tag="PLO65_07820"
     CDS             complement(5174..>6025)
                     /locus_tag="PLO65_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRD28191.1"
                     /translation="VQDALTQWWYGHNAVGFFLTAGFLAMMYYFVPKRIGRPVYSYRL
                     SIVHFWALIFIYIWAGPHHLHYTALPQWAQTLGMTFSIMLWMPSWGGMINGLMTLSGA
                     WDKLRTDPVVRMMVVAIAFYGMSTFEGPVMSIRAVNALSHYTDWTIGHVHSGALGWVG
                     FITFGAVYCLVPWLWKKDRLYSDKLVEWHFWIATTGIVLYICAMWVSGIMEGLMWREY
                     TPQGFLAWSFIETVSAKHVENIIRTIGGLMFLTGTLIMIYNLWRTVRLPSVETSDALS
                     APLPAAA"
BASE COUNT          987 a         1982 c         2154 g          902 t
ORIGIN      
        1 ggcgagaagc tgcggcggtc cgtcaagaag catcggccgc ccctcggtca ggacgccggt
       61 cttgtcgaac accacatggt cgacctcggc cagccgttcg agcgccgctc ctgtcttgac
      121 cagcacgccc ttccggaaca ggcggccgga agccacgatc tgcacggccg gcacggccag
      181 gcccagggcg caggggcagg tcacgatcag caccgcagcg gcgcgcagca acgcctcgcg
      241 ggggcccagg cccagcgccc agccgcccgc gaaggtcagg gccgccaggg tgtgaactac
      301 cggcacatag agggcggcgg ccttgtcggc gagctggaca tagttggacc ggctctgggc
      361 gccggcctcc atcagccggg cgatggcggc gacggccgag tcttccgagc gggccagcac
      421 gcgcaggacc aggcggcccg aaaggttcag cgcgccggcg ttgaccaccg agccggggcc
      481 gacgatgacg ctggcggtct cgcccgtgat cagggcgttg tcgagatcgg acccgcccgc
      541 ctcgaccagc gcgttgaccg ggatgcggtc gccggggacg accacgaggg cgtcgtccac
      601 ttcgacctcg ccaaccggaa tggcccgttc cacgccgccg gcctcgagcc gggtggccac
      661 gggcgcctgc agggccatca ggtcgcgggc ggcgctacgg gccctggctc gcagcttgtg
      721 gtccaggtag cggccgatca gcagcaggaa cagcagggtc acggccgcgt cgaagtaggc
      781 gtgggcgccg tgctgcaggg tctcggagaa gctgacgatc agggtcagca ggacgccgat
      841 cgagatcggc acgtccatgt tcgcccgccc cttgcgcagc gaggcccagg ccgaccggaa
      901 gaacggctgg ccggcgaaca gcgcgcaggg cgtcgcgatg gcggcggcga accagtacat
      961 cagctggcgg gtcgcggggt ccagttcctg gccgaagaag ccggcccaga ccgggacggt
     1021 gaacatcatc gcgttgcccg cgccgaaggc ggccacgccc agcgccaggg ccagggcgcg
     1081 gccctcgcga tcatggctgg cctcggcctt gccggggtcg aacggcgtcg ccggataacc
     1141 ctccttctcg agcgcctcga tgatcaggcc cgggtcgccg tcgcggcccg cgaaccggac
     1201 cgacagcttc ccggtcgtca ggttgaatcg cgccgcatcg acgccgggga gcgcggccgt
     1261 cacccgttcg atcttgccca tgcaggcggc gcagcgggcg ccggtgacca gcagctcgac
     1321 ccgtccctgg tcggtctcgt catgggtgac gaaggccgac agatcgcgat gggccagggc
     1381 gatggtcatc gccgcaccag ccggcgatcg gcctcgaaca ctgcgtcgcc ggctccacgg
     1441 atcgtcacgt gcagatccca ggcgccgtcc agcggtgcgg cgttggcccg gtacaggccg
     1501 gcgccggcgg cggtgaaggt caccgtgcgg cggcccttga tcgtggcggg gcgctccagc
     1561 gcgccgctga ccgcgaggcc gtcgatcggc ttgccggccc ggtcggccac gcggaccagc
     1621 acgatgtcgt cgcgcccttc ctcgatggtc gccgaccagc ccagggccgc ctctcgccgc
     1681 cgctgggcga gcgtgcggtt gtacagcaga ccggtctcgt agggattgct cgccgcctcg
     1741 ccggagaagg tgcgataggc caggctgatg aacaccgcgt cgacggcgat aacggcgccg
     1801 aagaacagga tcaggcccgc cagaacgtgc cagcccttga tggtgaaccg gggggtggtc
     1861 gccagatcgg tcatcgtgcg ttcgcctctc cggacaggaa gttggtgcgg gcctcggccg
     1921 tccccacgtc cgacttgatg gtgaaggcca ccggcaggtt ggtggcgggc agggaccgcg
     1981 ccggcgcact gacgaagacc cgcacggcgc ggacttcatc gggttcgacc gtgaccgtca
     2041 acagcccggt ggaggccgat tcgcccggcg tcttgagcgt cgcgtcgcgg atacccgaca
     2101 ggccgacagc gaaggactgg gggccgaagc tgcggttggt gatcttgagc gtgtagccgt
     2161 tgcggatcga gccgtcgtgc aggcgcacga aggtcggatt gcggtcgcgc aacacatgca
     2221 gatcgacggg agaccgctgc aggaagcccc agagcatgac cgcgctcacc agcgtcaggg
     2281 ccagggcgta gtagaccgtc cgggcccgga ccggatgata ggtcggcttg cggccctcga
     2341 gtcgcgagac cacggcggcg tccgtgtcat aggcgatcag gcccttcggc cggcccatcc
     2401 tgaccatgat ctcgtcgcag gcgtcgacgc acaggccgca gttgatgcac tccagctgcg
     2461 agccattgcg gatgtcgatg cccattgggc agaccacgac gcactggttg cagtcgacgc
     2521 agtcgccacg cccctcccag gtctcgttct tcttgtgcgg tccccgcggc tcgccgcgat
     2581 ccttcagata ggtgacctgc aacgagtgct ggtcgagcat ggcgccctgg atgcggggcc
     2641 aggggcacat gtaggtgcag acctgctcgc gcatggcgcc ggcgaaaccg taggtggtcg
     2701 cggtcagcag gccgcaggaa atgtacgcgg tcagcggcgc ctggccgagc cagaaggtgc
     2761 ggatcagcgt cggcgcgtcg tgaaaataga agatccaggc gcccccggtg gagaaggcga
     2821 tgcccagcca ggccaggtgc ttcccggtct tgcgccagac cttgttgaac gacatcggcg
     2881 aggcgtcgag cttcatccgc gcgttgcgat cgccctcgaa caggcgctcc acggcgatga
     2941 acaggtcggt ccagacggtc tgcggacagc tgtagccgca ccacaggcgg ccaaacaggg
     3001 ccgtgaccag gaacagcgcc aaggccccca tcaccagcag gccggtgatg aaatagacct
     3061 cctggggcca gagctggatg aagaagaagt agaaccggcg gccggtgaaa tcgaccagca
     3121 cggcctggtc cggcaattcg cccggacggg tccagcggag ccagggcacc acatagtaga
     3181 ccgccagggt ggcgatcagc atcgcccatt tgatggttcg ccactttccg tggaccagct
     3241 tgggatagat cggcgcgcgt ttctgataga gaccgccgcc ccggcccgcc ttgcggcggg
     3301 ccagagcggc ggcactgacc ggacccgggc ttttggccgc gctggggggc gtggtccggt
     3361 caattacaac ggtcatgacg gtaactactg acccgagttg gcgtggatgt agaccgcgag
     3421 ggccttgatg gtctcggggt cgaagcgatg gccccaggcc ggcatgacgc cgccgcggcc
     3481 attctggatc tggcccgaga tggccgcccg gtcggatccg tagagccagt cggcatcggt
     3541 caggtccggg gcgcccaggg ccatgtcgcc cttgccggcg acgccgtggc agctggcgca
     3601 ctgttcggcg aagaccggcg tggcccgacc gacggccgag gcattggccg gacgcccgga
     3661 cagggcgacc acatattcgg tcaggtcggc gatctgcgcc gcctgcagca tgccgtcgcg
     3721 gccgaaggcc ggcatctgcg acacccgcgc ctcgggatcc ccggagcgga tgccgtaggt
     3781 gatggtgcgc tggatgtcct ccagcgtgcc gccccacagc cagacgtcgt cgcgcaggtt
     3841 ggcgtagccc ttcccgccgc cgccgccgct gccgtggcag gtggcgcagt tgtcaccgaa
     3901 caccgactgc ccgacctgca gggcgtaggc ctgcagctgg ggatcgcttt cgatctgggc
     3961 cagggcggcc gccttgagct tggcgccgcc ggcgccgcgg gtgaccgcca ggtcctcgag
     4021 ggccctggcc acctcgcgcc ggtcggactt gccgagcagg ccctgggtgt aggtgttcag
     4081 gccgggccag gccggcatcg ccacccagta gccgatcgag aaggcgatgc aggcgtagaa
     4141 gacccacagc caccagcgcg gcagcggatt gtccagttcg cggataccgt cccattcgtg
     4201 gccggtcatg tcgacgccgc tgatggcgtc cttttcgcgg gcgtcacccg tcatagctct
     4261 tcctcctcga ggggcaggcg cgcggcgcgg gcgaactcgt cgccgttctt cggccagagg
     4321 gcgtaggcgc agcctcccag gaagagcagc gcgaagtaga ccagccctcc ctgttgggcg
     4381 atgcgcgcga cggtttcgta ggtcatcgtc aggcgctccc tagcgttggt tggcaggcgc
     4441 ggcggcctga taggtgctga agtcgaccag ggtgccgagc atctgcaggt aggcgatcag
     4501 ggcatccatt tcggtcagcc tttccgggtc gccgtcgaag tcgcggttga ccacctttgc
     4561 gccgtagcgg gcgcgcaggg ccggagcgtc ggacgagaac ggatcggcct gggcctgcag
     4621 gtcggtcatg gtctggtcga tctgctcctg ggtgtagggc acgccgacct tggtcatcgc
     4681 cgccagcttg gcctgcaggt cccgcgtctg caggtccttg gtggccagga aggcgtaggg
     4741 cggcatgacc gactcgggca ccaccgaccg cggatcgatc agatggtcgc ggtgccagct
     4801 gtcggagtac ttgcccccga cccgggccag gtcaggcccg gtccgcttcg agccccactg
     4861 gaaggggtgg tcgtacatgc tctccgcggc caggctgtag tggccgtagc gctcgacctc
     4921 gtcgcgcagg ggacggatca tctgcgagtg gcagagatag cagccctcgc ggatgtagat
     4981 gtcccgtccg gccagctcga gcggggtgta ggggcggacc ccgtcgacct tctcgatggt
     5041 gttcttgatc cagaacagcg gggcgatttc gaccaggccg ccgacagaga cgaccaggac
     5101 gatgccgatg acgagcagca gcgaatgccg ctcaagtttt ccgtgatgct tccacatgct
     5161 catggcgcgg tcctcaagcg gctgccggga gcggcgcgga gagcgcgtcg ctggtttcga
     5221 cggacggcag ccggaccgtg cgccacaggt tgtagatcat gatgagcgtg ccggtgagga
     5281 acatcaggcc gccgatggtg cggatgatgt tctcgacgtg cttggccgag accgtctcga
     5341 tgaagctcca ggcgaggaag ccctgcgggg tgtattcccg ccacatcagg ccttccatga
     5401 tgccggagac ccacatcgcg cagatgtaga gaacgatgcc cgtggtcgcg atccagaagt
     5461 gccactcgac gagcttgtcg gagtagagcc ggtccttctt ccacagccag ggcaccaggc
     5521 agtagacggc gccgaaggtg atgaagccga cccagccaag agcgccggag tgcacgtggc
     5581 cgatggtcca gtcggtgtag tggctgagcg cgttgaccgc ccggatcgac atcaccggac
     5641 cttcgaaggt cgacatgccg tagaaggcga tggccacgac catcatccgc acgaccgggt
     5701 cggtgcgcag cttgtcccac gcccccgaga gggtcatcag gccgttgatc atgccgcccc
     5761 agctgggcat ccacagcatg atcgagaagg tcatgccgag ggtctgcgcc cactgcggca
     5821 gcgccgtgta gtggaggtgg tgcggcccgg cccagatgta gatgaagatc agggcccaga
     5881 agtgcacgat cgacagacgg tagctgtaga ccggccggcc gatccgcttc ggcacgaagt
     5941 agtacatcat ggccaggaag ccggccgtca ggaagaagcc gaccgcgttg tggccgtacc
     6001 accactgggt cagggcgtcc tgcac
//
LOCUS       DAORNX010000145         6023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9744_length_6023_cov_7.716655, whole genome shotgun sequence.
ACCESSION   DAORNX010000145 DAORNX010000000
VERSION     DAORNX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9744_length_6023_cov_7.716655"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..625
                     /locus_tag="PLO65_07825"
     CDS             245..625
                     /locus_tag="PLO65_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28192.1"
                     /translation="MRPVLAAAVSAVLMALAGTAGAAEPGCFDPAGGESLADSRWTGT
                     SEWQGLAANPMTLHLRPDCTVEYVSRGVTYANGTWLQRGALIEWQANDRFAVYVGQVG
                     EGQIGGVMYNRRGQTGVWRFKRAD"
     gene            726..851
                     /gene="ykgO"
                     /locus_tag="PLO65_07830"
     CDS             726..851
                     /gene="ykgO"
                     /locus_tag="PLO65_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRD28193.1"
                     /translation="MKVRSSLKSLKNRHRDCKLVRRKGVLYVINKTDPRFKAKQG"
     gene            854..1468
                     /locus_tag="PLO65_07835"
     CDS             854..1468
                     /locus_tag="PLO65_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRD28194.1"
                     /translation="MRTPRGVLWDVGNVVVGWSPRRLYEKIFEEPAACDRFLSSVCTL
                     DWHIEHDRGVSFRDNAAPLIARHPELESEIRAWGERFVEMVGPVFADTEAAIHALAAR
                     GVPQFGLTNMPSEGWPAVQALSPAFGHFRDTVVSGDEGLIKPDPAIFRLASERAGMAP
                     EDLLFIDDSPANIAAARALGFHVHRFEDPAHLNSLLVGHGLLEA"
     gene            1529..1795
                     /locus_tag="PLO65_07840"
     CDS             1529..1795
                     /locus_tag="PLO65_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HRD28195.1"
                     /translation="MADDASTIDVLNSTAQTQLKTILERIERLEEDKAAVMADLKEVY
                     AEAKGNGFDVKIIRKVVRLRKQDRAKRQEEEALIDLYMSAIGEA"
     gene            1792..2109
                     /locus_tag="PLO65_07845"
     CDS             1792..2109
                     /locus_tag="PLO65_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28196.1"
                     /translation="MKRGAPDLKAQARRAALNALRRARRTADKAGVDLSDWEGEFLGS
                     VEDRLKTYGRAFGDPEKGAAGQALSVLQTVKLKEITAKARGEKSPMKRGAFGRRSPPA
                     KPE"
     gene            2114..2830
                     /locus_tag="PLO65_07850"
     CDS             2114..2830
                     /locus_tag="PLO65_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HRD28197.1"
                     /translation="MILRSVLTVLLTLAAGCAVPESVAPAAAGAACLLPARVTPAAAY
                     DPPADEIVADAPTAFYMLALTWAPEDCRANGDRPGYEIQCRDNAFGFVLHGLWPNGEG
                     GRHPRYCRPAPAISPATVRANACMTPSTVMQQHEWAAHGTCGWTTPEAYFGQAAALWR
                     AVRPPATPESAATAGALRDAFVAANPWIPRDGVYIKTVEGGRLMDVRLCYDLAYRPMA
                     CRGSLGARDDTLLTVTPRRP"
     gene            complement(2843..3871)
                     /locus_tag="PLO65_07855"
     CDS             complement(2843..3871)
                     /locus_tag="PLO65_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRD28198.1"
                     /translation="METAVDLAALAAWMDGQGLGAGPVEDATLLAGGTQNILLKFSRA
                     GRSYVLRRPPVHLRANSNETMRREARVLAAIAGTDVPHPGLIAACGDETVLGAAFYLM
                     EPVDGFNPTTGLEPLHAGSETIRRRMGLSLVEGIAALGALDYRARGLEGLGKPDNYLE
                     RQVARWKAQLESYGEFDGWTGPGEIPGVDRVGAWLDAHRPAGFQAGIIHGDYHLANVM
                     YRHDSGDLAAIVDWELTTIGDPLLDLGWLLATWPEGDDPDPANVSVRPWKGFPTAAEL
                     VEHYRARTTRDLAAIHWYGVLACYKLGIILEGTHARACAGKAPVATGDRLHAQTIGLF
                     ERALRWIG"
     gene            complement(3947..4069)
                     /locus_tag="PLO65_07860"
     CDS             complement(3947..4069)
                     /locus_tag="PLO65_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28199.1"
                     /translation="MARRVNIRPPQPRDARLKTLMRRMKLAAARVTVLPAAAAV"
     gene            complement(4069..5523)
                     /locus_tag="PLO65_07865"
     CDS             complement(4069..5523)
                     /locus_tag="PLO65_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRD28200.1"
                     /translation="MTKTVLVVDDDPTQRRLIQAVLEREGFAVAQAEDGDSALKHLAM
                     GQPADVVLLDLQMPGLNGQDALKEMRARGHMQPVIVLTAGGGVETVVKAMQAGAGDFF
                     VKPASPERIIVSIRNVLSMGEMRTEVDRLKKHASGRTTFADLIGDSAPMQMVKRLGER
                     AARSSIPILITGESGVGKEVIARAVHGASDRAGKPFVAVNCGAIPENLVESILFGHEK
                     GSFTGASEKHLGKFREADGGTLFLDEVGELPLDMQVKLLRALQESEIDPIGAKRSVRV
                     NVRIVSATNRDLSNAVAEGRFREDLFYRLNVYPIEAPSLRERREDIPALIEHFIARFN
                     VEEGKRVMGASPETLAILSGFDWPGNVRQLENAVYRAIVLSDSPHLQPHDFPAISGVK
                     VAPEAWSQPVDPPTAQQLIAEISKDAPVRILDGRGHVRPLEEVERDLIQLAIEIYAGH
                     MSEVARRLGIGRSTLYRKVREQGLDVEGGMEEAG"
     gene            complement(5635..>6023)
                     /locus_tag="PLO65_07870"
     CDS             complement(5635..>6023)
                     /locus_tag="PLO65_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD28201.1"
                     /translation="AHRRYAERHGLPRTIDALTGHPLIGFDRDDMARRSIAADGPMVS
                     RDAFAFRCDSDLAQLAALRAGFGIGGCQVGIAADDADLLPVLPDQVRFELEMWLAMHE
                     DLRPVARVRAMYDHLATELAAYARRR"
BASE COUNT          908 a         2128 c         2096 g          891 t
ORIGIN      
        1 cgcgggcgtg acgggcgcgc ccggcgccgg cggggcgccg ggcgaagcga tccccagcga
       61 cggctccagc gaggggtcgg cgcagccggc gacgatcagg gcgagaaatg aacgacgatc
      121 catcgggcca cgctacaggg gtgcggggct gcacctcaat cacaagcctg cgaataccat
      181 gccgagattg accgcatgcg gccggccacg ggataatccg tccagggatt gtgggggtct
      241 gatcatgcgt ccagtgctcg cggcggctgt ctcggcggtg ttgatggccc tggcgggcac
      301 ggccggcgcc gcggagccgg gctgtttcga cccggcgggc ggagaatccc tggccgacag
      361 ccgctggacc ggaacctccg aatggcaggg cctggcggcc aacccgatga ccctgcacct
      421 gcggcccgac tgcacggttg aatatgtctc caggggcgtc acctacgcca acggaacctg
      481 gctccagcgc ggcgccctga tcgagtggca ggccaacgac cgtttcgccg tctatgtcgg
      541 gcaggtcggc gagggccaga tcggcggggt gatgtacaac cgccggggcc agacgggcgt
      601 ctggcggttc aagcgcgcgg attgactcaa aacccgtcgc cgcctatagc cgcccgctct
      661 ggaacaaccg ggtttccgtc ggcgccgcgt ttcgcgcccg gatcacccgc aaacggtaga
      721 cggtcatgaa ggtcagaagc tcgctcaagt cgctgaagaa ccgccaccgc gactgcaagc
      781 tcgtgcgtcg caagggcgtg ctgtacgtga tcaacaagac cgacccgcgc ttcaaggcca
      841 aacagggctg agcatgcgca cgccccgggg cgtactctgg gacgtcggca atgtggtcgt
      901 gggctggagc ccgcggcgcc tctacgagaa gattttcgag gaaccggccg cctgcgaccg
      961 gttcctttcg tctgtctgca cgctcgactg gcacatcgaa catgatcggg gtgtgtcgtt
     1021 ccgggacaac gccgcgccgc tgatcgcccg ccatcccgag ctggagtcgg aaatccgcgc
     1081 ctggggcgag cgcttcgtcg agatggtcgg gccggtgttc gcggacaccg aggcggccat
     1141 ccacgccctg gccgcccgcg gcgtgccgca gttcggcctg accaacatgc ccagcgaggg
     1201 ctggccggcc gtccaggcct tgtcaccggc gttcggccat ttccgcgaca ccgtggtttc
     1261 cggcgacgag gggctgatca agcccgatcc ggccatcttc cgcctggcca gcgagcgggc
     1321 cggcatggcc ccggaagacc tgctgttcat cgacgactcg ccggccaaca tcgccgccgc
     1381 gcgcgcgctg ggcttccatg tccacaggtt cgaggacccg gcccacctca attcgctgct
     1441 ggtcggccac ggactgcttg aggcctgacc ccggggcggc tagaccgtcc gccttcccga
     1501 tccctccgtc ctgtgagagc ttctccccat ggccgacgac gcctccacca tcgacgtgct
     1561 caacagcacg gcccagacgc agctcaagac cattctcgag cgcatcgagc ggctggagga
     1621 ggacaaggcc gcggtgatgg cggacctcaa ggaagtctac gccgaggcca agggcaacgg
     1681 cttcgacgtc aagatcatcc gcaaggtggt gcgtctgcgc aaacaggacc gggccaagcg
     1741 ccaggaggaa gaggcgttga tcgacctcta catgtcggcg atcggagaag cttgaagcgc
     1801 ggcgcgccag atctgaaggc gcaggccagg cgggccgcgc tgaacgccct caggcgggcg
     1861 cgccgcacgg ccgacaaggc cggtgtcgac ctgtccgact gggagggcga attcctcggc
     1921 agcgtcgagg atcgcctgaa gacctacggc cgggccttcg gcgaccccga gaagggcgcc
     1981 gccggccagg ccctgtcggt gctgcagacc gtgaagctca aggagatcac ggccaaggcc
     2041 aggggagaga agtcgccgat gaaacgcggg gccttcggcc gccggtcccc gcccgccaag
     2101 ccggagtgac aggatgatcc tccggtccgt ccttacggtc ctgctgacgc tggccgccgg
     2161 ctgcgccgtt cccgaatccg tcgcgccggc cgccgccggc gccgcctgcc tgctgccggc
     2221 cagggtcact cccgccgcgg cctacgaccc gccggccgac gagatcgtcg ccgatgcgcc
     2281 gaccgccttc tacatgctgg ccctgacatg ggccccggaa gactgccggg ccaacggcga
     2341 ccggccgggc tacgagatcc agtgccggga caacgccttc ggcttcgtgc tgcatggcct
     2401 gtggcccaac ggcgagggcg ggcgccatcc gcgctactgc cggccggcgc ccgcgatcag
     2461 ccctgccacc gtccgggcca acgcctgcat gacgccctcg accgtgatgc agcaacacga
     2521 atgggccgcg cacggaacct gtggctggac gacgccggag gcctatttcg gccaggcggc
     2581 ggctctctgg cgggcggtgc gtccgcccgc gaccccggaa tcggccgcga ccgcgggcgc
     2641 cctgcgggat gcgttcgtgg cggccaatcc ctggatcccg cgggacggcg tctacatcaa
     2701 gacggtcgag ggcgggcggc tgatggatgt gcgcctgtgc tacgatctcg cctaccggcc
     2761 catggcctgc aggggctcgc tgggcgcgcg ggatgacacg cttctgaccg tcacaccccg
     2821 gcggccctga ccgggccgcc gctcacccga tccagcgcag cgcccgctcg aacaggccga
     2881 tggtctgggc gtgcaggcgg tcccctgtgg cgacaggcgc cttgcccgcg caggcccggg
     2941 cgtgggtgcc ctcgaggatg atgcccagct tgtagcaggc cagcacgccg taccagtgga
     3001 tcgccgccag atcccgtgtc gtgcgcgccc ggtagtgctc gaccagctcg gcggcggtcg
     3061 ggaaaccctt ccacggcctt accgagacat tggccggatc ggggtcatcg ccttccggcc
     3121 aggtggccag cagccagccc aggtcgagca ggggatcgcc gatggtggtc agctcccagt
     3181 cgacgatggc ggccaggtcg ccgctgtcat ggcggtacat gacattggcc aggtggtagt
     3241 cgccatggat gatcccggcc tggaaaccgg ccggccggtg cgcgtccagc caggccccga
     3301 cccggtcgac gccggggatt tcgccgggcc cggtccagcc gtcgaactcc ccgtaggact
     3361 ccagctgcgc cttccagcgc gcgacctgcc gctcaaggta gttgtcgggc ttgccgagcc
     3421 cctcgaggcc tcgggcgcga tagtcgagcg cgccgagggc ggcgatgccc tcgaccagcg
     3481 acaggcccat gcgtcgacgg atcgtctccg acccggcgtg cagcggttcc aggccggtgg
     3541 tcgggttgaa gccgtcgacc ggctccatca ggtagaaggc cgcgcccagc acggtctcgt
     3601 cgccgcaggc cgcgatcagg cccggatgcg gcacgtcggt cccggcgatc gccgccagca
     3661 cccgcgcctc gcgccgcatc gtctcgttgg aattggcccg cagatgaacc ggcgggcggc
     3721 gcagcacgta gctccgtccc gcgcggctga acttcagcag gatgttctgc gtaccgccgg
     3781 ccagcagcgt cgcgtcctcg accgggcccg cgcccaggcc ctgcccgtcc atccaggcgg
     3841 ccagcgccgc cagatccaca gccgtttcca tcccgccagt cagcgcgatt tccgccacgg
     3901 gaggcaaggc ccccgaacgg atacgccgcg ggaattgcgg gggttgctag acggccgccg
     3961 ccgccggcag gacagtcacc cgggccgccg ccagcttcat ccggcgcatc agggtcttca
     4021 gccgcgcatc acgaggctgg ggcggacgaa tgttcacgcg acgggccatc agccggcctc
     4081 ctccatgccg ccctcgacgt cgagaccctg ttcgcggacc ttgcggtaga gggtcgagcg
     4141 accgatgccc aggcggcggg cgacctcgct catgtggccg gcgtagattt cgatcgccag
     4201 ctggatcagg tcgcgctcga cctcctccag cgggcggaca tggccgcggc cgtcgaggat
     4261 gcgcaccggc gcgtccttgc tgatctcggc gatcagttgc tgggccgtcg gcgggtcgac
     4321 gggctgggac caggcctccg gcgcgacctt gacccccgag atggccggga agtcgtgcgg
     4381 ctgcaggtgc ggcgagtcgg acagcacgat ggcgcgatag accgcgttct ccagctggcg
     4441 gacgttgccg ggccagtcga agccgctgag gatggccagg gtctcgggcg aggcgcccat
     4501 gacgcgcttg ccctcctcga cattgaaccg ggcgatgaag tgctcgatca gggccgggat
     4561 gtcctcgcgg cgctcgcgca gggacggggc ctcgatcgga tagacgttga gccggtagaa
     4621 caggtcctcg cggaagcgtc cctcggccac ggcgttgctc aggtcgcgat tggtcgccga
     4681 gacgatgcgc acattgaccc gcaccgaccg cttggccccg atcgggtcga tctcgctctc
     4741 ctgcagcgcc cgcagcagct tgacctgcat gtccagcggc agctccccga cctcgtcgag
     4801 gaacagggtc ccgccgtccg cctcgcggaa cttgcccagg tgcttttccg acgcgccggt
     4861 gaagctcccc ttttcgtggc cgaacaggat cgattccacg aggttctcgg ggatcgcccc
     4921 gcagttcacc gccacgaacg gcttgccggc ccggtcgctg gcgccgtgca ccgcgcgggc
     4981 gatgacttcc ttgcccacgc cgctctcgcc ggtgatcagg atcgggatcg aggagcgggc
     5041 cgcccgctcg cccagccgct tgaccatctg catcggcgcg ctgtcgccga tcaggtctgc
     5101 gaaggtggtg cggcccgacg catgcttctt caggcggtcg acctcggtgc gcatctcgcc
     5161 catcgacagc acgttgcgga tcgagacgat gatccgctcg gggctggccg gcttgacgaa
     5221 gaagtcgccg gccccggcct gcatcgcctt gaccaccgtc tcgaccccgc cgccggcggt
     5281 cagcacgatc accggctgca tgtggccgcg ggcgcgcatc tccttgagcg cgtcctggcc
     5341 gttgagtccg ggcatctgca ggtcgagcag caccacgtcc gcgggctggc ccatggccag
     5401 gtgcttgagc gcgctgtcac cgtcctcggc ctgggcgacg gcgaagccct cgcgttccag
     5461 caccgcctgg atcagacgcc tttgcgtcgg gtcgtcgtcg acgaccagga ccgttttggt
     5521 catatgaaga tcacccaccg taccggcgtg cgcccgacat ggagcgccgc tcttgttgac
     5581 ccggattgaa acacgggccg gtaaagaccg ggtttcggag acctgagtcg ggggttaacg
     5641 gcggcgcgcg taggcggcca gttcggtggc cagatgatcg tacatcgccc gcacccgggc
     5701 gaccggccgc aggtcctcgt gcatggccag ccacatttcc agctcgaacc ggacctggtc
     5761 gggcagcacc ggcagcaggt cggcgtcgtc ggcggcgatg ccgacctggc agccgccgat
     5821 gccgaatccg gcgcgcagcg cggccagctg ggcaaggtcg ctgtcacacc ggaacgcgaa
     5881 ggcgtcgcgg ctgaccatcg gaccgtccgc ggcgatcgag cggcgggcca tgtcgtcgcg
     5941 gtcgaagccg atcagcggat ggcccgtcag cgcgtcgatg gtccgcggca ggccatgccg
     6001 ctcggcgtag cgacggtggg cga
//
LOCUS       DAORNX010000146         5995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9809_length_5995_cov_8.701010, whole genome shotgun sequence.
ACCESSION   DAORNX010000146 DAORNX010000000
VERSION     DAORNX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_5995_cov_8.701010"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PLO65_07875"
     CDS             complement(<1..174)
                     /locus_tag="PLO65_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD28202.1"
                     /translation="MKTIVVTSRKGGVGKTTVAVNMALAAFGGGARVLLADTDPQRSA
                     TDALKARARPGPAL"
     gene            375..1103
                     /locus_tag="PLO65_07880"
     CDS             375..1103
                     /locus_tag="PLO65_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRD28203.1"
                     /translation="MKRAFEFLMNMDARAWRTVVVAFLLFGGVGTVFLFAAPLLGLNG
                     EATVEHWLSAAQGPWALPVAISAFAVLAFIGVPQFVLIAAAVVAFGPWTGLTYSWIGT
                     LVSSSIGFWLGRAFGARLIRDVASEGLGRFMKLIRRNGFMASLIVRLVPAAPFIIVNM
                     AAGVARMRFRDFVGGTAIGIIPKIALTAFAGNSAVQFLKSGGLQHIVLIALAAVIWVG
                     GAWVARGWLRRREDAAEREDAAGE"
     gene            1177..3123
                     /locus_tag="PLO65_07885"
     CDS             1177..3123
                     /locus_tag="PLO65_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRD28204.1"
                     /translation="MRRLLAAVTAFLVLAGPAVAADAPSAATVKAQAALKKTLPFEDR
                     QDFDFASRGYLGTLADPVIHRADGAVVWDLSAYDFLQGDAPDTVNPSLWRHAQLLAKS
                     GLFQVSDTIWQVRGFDLSNITFIKGETGWIVIDPLTTAEVAKAAYALVTEKLGARPIV
                     AVIYTHSHTDHFGGVKGVISQADVDAGKVQVIAPQGFLEEAVSENVIAGVAMSRRATY
                     QFGLGLTAGPEGLVNSGIGKAVSKGTITLIAPTVSIDHTGQTLVVDGVKIEFQITPGT
                     EAPAEMNLYFPDLRILCMAENANVTMHNVLTPRGALVRDSRAWAAYLSESIRLFGDRT
                     DVMFTSHGWPRFGGEVVRDFLADHRDAYKYLHDQTVRLMNQGYVGEEIANRIALPPAL
                     AREWFNRGYYGNMSFNSRAVYQRYMGWYDANPAHLAVLPPADEAGRYVEAMGGADKVR
                     ALAQAAYDKGDYRWAATLLNNLVLSTPGDAAAKEALARTYDQMGYQAESALWRNMYLT
                     GADELRGGIKPALAAGSTDMIANLPSPMIFDLLAVRLDPAKAGDAELRILFVFTDRNE
                     RYLLQVRHGVLTAETAPEGVKADATLTLPRGLLMQSLFTGAPLAPKVASGEAKIAGNP
                     FALQKLVGWFDKPAGAFPIVTRPE"
     gene            3135..3785
                     /locus_tag="PLO65_07890"
     CDS             3135..3785
                     /locus_tag="PLO65_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD28205.1"
                     /translation="MMTLHYFPGSCSLASHIALEEAGADYRAVRVDLRAGQQRSDDYL
                     ALNPLGRVPVLVVDGVALVENAAILAFIAQRFPAAGLAPVDDPLAFARVQGFNLFLAS
                     TVHPAHAHGVRGYRWADDPAAIAAMKAKVPQVIGDCFDLIERDGLLGPWVMGEAYGIC
                     DPYLFTLARWLEEDGVDPARWPKVEAHRRRMAERPAVRKVLADEGAVEPLWRKTVA"
     gene            3963..5531
                     /locus_tag="PLO65_07895"
     CDS             3963..5531
                     /locus_tag="PLO65_07895"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRD28206.1"
                     /translation="MTAPVTAASRDRVIIFDTTMRDGEQSPGASMSHDEKLELAKILE
                     EMGVDVIEAGFPIASNGDFEAVRAIAQIVTESTVCGLARAAAADIDRCAEAIRPARRG
                     RIHTFISTSPQHRDYILKLSQDEVLEAVVQSVTRARNLCDDVEWSAQDATRTEPEFLY
                     RCVDAAIRAGARTINLPDTVGYSFPEEYAAMFRNVILNVPDSDRAIFSAHCHNDLGLA
                     VANSIAAVQGGARQVECAINGIGERAGNAALEEIVMALKVRGETLGFDSQVDPVHITR
                     ASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAETYEIMRPEDVGQGATNL
                     VMGKHSGRHAFREKLKALGYDLGQNALNEAFVRFKELADKKKHVFDDDLIALVDDALA
                     RGSERIRVVHLRVVAGTEGQSAELTLAVDDVEKKAEASGDGPVDAVFNAIHEIVPHKA
                     ALRLFQVHAVTEGTDAQAQVSVRLEEDGRIATGQAADTDTLTASAKAYVNALNNLFSR
                     KEKGRPDTAIASGF"
     gene            5541..>5995
                     /locus_tag="PLO65_07900"
     CDS             5541..>5995
                     /locus_tag="PLO65_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRD28207.1"
                     /translation="MLWIPVTVAAAAFQVARNAAQRSLLGGAGPWGATLVRFLFGLPF
                     SILFSSVALLLLPARLEVTPVTLAWAALGGAMQLGATAALLTAMHRSSFALGTAFQQS
                     GLPFAALLGLLFFGDHLSAGGWGGVLLASAGLLVLSWPRGGEAARDWTAA"
BASE COUNT          896 a         2131 c         2042 g          926 t
ORIGIN      
        1 cagggccggg ccagggcgcg cgcgcgcctt cagggcgtcg gtcgccgagc gctgcggatc
       61 ggtgtcggcc agcagcaccc gggcgccgcc gccgaaggcc gccagggcca tgttgaccgc
      121 caccgtcgtc ttgccgacac cgccctttct cgatgtcacg acaatagtct tcatccgtgt
      181 ctccggccgc gcgcggcggt gagtgtcacc gctgaccgtt tcaggcgcgt gacaggggtc
      241 aatggccggg gcgcccgatc tggggcggcg cgcgtcatct gcccgtggcg cgggcgtcga
      301 ccgcgccggg cgcccgccgc gaatgcgccg tgatcctgct tgaccctccc gacctgatcg
      361 gcgaggcctt ggcgatgaag cgcgccttcg agtttctcat gaacatggac gcccgggcgt
      421 ggcggaccgt ggtcgtcgcc ttcctgctgt tcggcggcgt gggcacggtg ttcctgttcg
      481 ccgcgccgct gctgggcctg aacggcgagg cgacggtgga gcactggctc agcgccgccc
      541 aggggccctg ggcgctgccg gtggccatca gcgccttcgc cgtgctggcc ttcatcgggg
      601 tgccgcagtt cgtgctgatc gccgccgccg tggtcgcctt cggaccctgg accggcctga
      661 cctacagctg gatcggcacc ctggtgtcct cgtcgatcgg cttctggctg gggcgggcgt
      721 tcggcgcgcg gctgatccgc gacgtggcca gcgaggggct tggccgattc atgaagctga
      781 tccggcgcaa cggcttcatg gccagcctga tcgtgcggct ggtgccggcc gcgccgttca
      841 tcatcgtcaa catggccgcc ggcgtggcgc gcatgcgctt ccgcgatttc gtcgggggca
      901 cggccatcgg catcattccc aagatcgccc tgaccgcctt cgccggcaat tcggcggtgc
      961 agttcctcaa gagcggcggg ctgcagcaca tcgttctgat cgccctggcg gcggtgatct
     1021 gggtcggcgg ggcctgggtg gcgcgcggct ggcttcgtcg ccgcgaggac gcggccgaac
     1081 gcgaggatgc cgccggggaa taattgtaac ggcggtgaca gtcattcgtc accgatccgc
     1141 taccaccgga cacaatcgat gatccgggag gattccatgc gcagactgct cgccgccgtt
     1201 acggcgttcc tcgtcctggc cggccccgcc gtcgccgcgg acgccccctc ggccgccacg
     1261 gtcaaggccc aggccgcgct gaagaagacc ctgccgttcg aggatcgcca ggacttcgac
     1321 ttcgcctcgc gcggctatct gggaaccctg gccgatccgg tgatccaccg cgccgacggc
     1381 gccgtggtct gggacctgtc ggcctacgac ttcctccagg gcgatgcgcc cgacaccgtc
     1441 aatcccagcc tgtggcggca cgcccagctg ctggcgaaga gcggcctgtt ccaggtcagc
     1501 gacacgatct ggcaggtgcg cggcttcgac ctctcgaaca tcaccttcat caagggcgag
     1561 accggctgga tcgtcatcga cccgctgacc acggccgagg tggcgaaggc ggcctacgcc
     1621 ctggtgaccg agaagctcgg cgcccggccg atcgtggcgg tcatctatac ccacagccac
     1681 accgaccact tcggcggggt caagggcgtc atcagccagg ccgacgtcga cgccggcaag
     1741 gtgcaggtca tcgcgccgca gggcttcctc gaggaggcgg tcagcgagaa cgtgatcgcc
     1801 ggcgtggcga tgagccgccg ggccacctac cagttcggcc tcggcctgac cgccgggccc
     1861 gagggcctgg tcaactccgg catcggcaag gccgtgtcca aggggaccat caccctgatc
     1921 gcgccgaccg tcagcatcga ccacacgggc cagaccctgg tcgtcgacgg ggtgaagatc
     1981 gagttccaga tcacgcccgg caccgaggcg ccggccgaga tgaacctcta cttccccgac
     2041 ctgcgcatcc tgtgcatggc cgagaacgcc aatgtcacca tgcacaacgt gctgacgccg
     2101 cgcggggcgc tggtgaggga ttcgcgcgcc tgggccgcct atctgtcgga gtcgatccgg
     2161 ctgttcggcg accgcacgga cgtgatgttc accagccacg gctggccacg cttcggcggc
     2221 gaggtcgtgc gcgacttcct cgccgaccat cgcgacgcct acaaatacct gcatgaccag
     2281 acggtgcggc tgatgaacca gggctatgtc ggcgaggaga tcgccaaccg catcgccctg
     2341 ccgccggccc tggcccgcga atggttcaac cgcggctact acggcaacat gagcttcaac
     2401 agccgggcgg tctatcagcg ctacatgggc tggtatgacg ccaatcccgc gcatctggcc
     2461 gtgctgccgc cggccgacga ggccgggcgc tatgtcgagg ccatgggcgg ggccgacaag
     2521 gtccgcgccc tggcccaggc cgcctacgac aagggcgact accgctgggc ggcgaccctg
     2581 ctcaacaacc tcgtgctgtc cacgcccggc gacgccgcgg cgaaggaggc cctggccagg
     2641 acttacgacc agatgggcta ccaggccgag agcgcgctgt ggcgcaacat gtacctgacc
     2701 ggcgccgacg aactgcgagg cgggatcaag ccggccctgg cggccgggtc gaccgacatg
     2761 atcgccaacc tgccgtcgcc gatgatcttc gatctgctgg cggtgcggct ggatccggcg
     2821 aaggccggcg acgccgaact tcgcatcctg ttcgtgttca ccgaccggaa cgagcgctac
     2881 ctcctgcagg tgcggcatgg cgtgctgacg gccgaaacgg cgccggaggg cgtcaaggcc
     2941 gacgcgaccc tgaccctgcc gcgcggcctg ctgatgcagt cgctgttcac cggcgccccg
     3001 ctggcgccga aggtcgcgtc gggcgaggcg aagatcgccg gcaatccgtt cgcgctgcag
     3061 aagctggtcg gctggttcga caagccggcg ggggccttcc cgatcgtcac acgaccggag
     3121 tagcctgcgg cgtcatgatg acgctgcact acttcccggg ctcctgctct ctcgccagcc
     3181 atatcgcgct ggaggaggcg ggggccgact atcgtgctgt gcgggtcgac ctgcgggccg
     3241 gccagcagcg gagcgacgac tatctggcgc tcaatccgct gggccgggtc ccggtgctgg
     3301 tggtcgatgg cgtggcgctg gtcgagaacg cggccatcct ggccttcatc gcccagcggt
     3361 tcccggcggc aggcctggcc cccgtcgacg atccgctggc gttcgcccgg gtgcagggtt
     3421 tcaacctgtt cctcgccagc accgtgcatc cggcccacgc ccacggcgtg cgcggctacc
     3481 gctgggccga cgacccggca gccatcgcgg ccatgaaggc caaggtcccg caagtcatcg
     3541 gcgactgctt cgacctgatc gagcgcgacg ggttgctcgg cccctgggtg atgggcgagg
     3601 cctacggcat ctgcgatccc tacctgttca ccctggcgcg ctggctggag gaggacgggg
     3661 tcgacccggc ccgctggccg aaggtcgagg cgcaccgccg acggatggcc gagcgcccgg
     3721 ccgtgcggaa ggttcttgcc gacgaggggg ctgtcgagcc gctctggcgc aagactgttg
     3781 cttgaatcgc cggattccgt tatccagaaa tcatgcaacg agatcgcgcc gatctgcttc
     3841 tactcgcgct ggggcttagc cgcccctggg cgtccgcctg accgctcgac cgcggcctgg
     3901 cgctcagggg cttattcgat ccgcaccctg atcccggcat ccgacctttg cagagtagac
     3961 ccatgaccgc ccccgtaacc gccgcctcgc gcgaccgcgt catcatcttc gacaccacca
     4021 tgcgcgacgg cgagcagtcg cccggcgcct cgatgagcca cgacgagaag ctcgaactgg
     4081 ccaagatcct cgaggagatg ggcgtcgacg tgatcgaggc cggcttcccg atcgcctcca
     4141 atggcgactt cgaggccgtc cgcgccatcg cccagatcgt caccgagagc accgtctgcg
     4201 gcctggccag ggcggccgcc gccgacatcg accgctgcgc cgaggcgatc cgtccggcca
     4261 ggcgcgggcg gatccacacc ttcatctcca ccagcccgca gcatcgcgac tacattctca
     4321 agctgtcgca ggacgaggtg ctggaggcgg tggtccagtc ggtcacccgc gcccgcaacc
     4381 tgtgcgacga cgtcgagtgg tcggcccagg acgcgacccg caccgagccg gagttcctct
     4441 accgctgcgt cgacgccgcg atccgcgccg gggcccgcac gatcaacctg cccgacacgg
     4501 tcggctacag cttccccgaa gagtacgccg caatgttcag gaatgtgatc ctgaacgtgc
     4561 cggactctga cagggcgatc ttctcggccc actgccacaa cgatctgggc ctggccgtgg
     4621 ccaactcgat cgcggccgtg cagggcgggg cgcgccaggt cgaatgcgcc atcaacggca
     4681 tcggcgaacg cgccggcaac gccgcgctgg aagagatcgt catggccctg aaggtgcgtg
     4741 gcgagaccct cggcttcgac agccaggtcg acccggtgca catcacccgc gccagccgct
     4801 acgtctcggc catcaccggc ttcccggtgc agttcaacaa ggccatcgtc ggcaagaacg
     4861 ccttcgcgca cgagagcggc atccatcagg atggcatgct caagaacgcc gagacctacg
     4921 aaatcatgcg gccggaggac gtggggcagg gcgccaccaa cctggtcatg ggcaagcact
     4981 cgggccgtca cgccttccgc gagaagctca aggccctggg ctacgacctg ggccagaacg
     5041 ccctcaacga ggccttcgtc cggttcaagg agctggccga caagaagaag cacgtcttcg
     5101 acgacgacct gatcgccctg gtcgacgacg ccctggcccg tggctccgaa cgcatccgcg
     5161 tcgtgcatct gcgcgtcgtg gccggcacgg aggggcagtc cgccgagctg accctggccg
     5221 tcgacgacgt ggagaagaag gccgaggcca gcggcgacgg tccggtcgac gcggtgttca
     5281 acgcgatcca cgagatcgtg ccgcacaagg cggcgctgcg cctgttccag gtgcatgcgg
     5341 tcaccgaggg caccgacgcc caggcccagg tctcggtgcg tctggaagag gacggccgca
     5401 tcgccaccgg ccaggccgcg gacacggaca cgctcaccgc cagcgccaag gcctatgtca
     5461 acgcgctgaa caacctcttc tcccgcaagg aaaaggggcg gcccgacacg gcgatcgcca
     5521 gcggattcta gcccgcttcc gtgctctgga tccccgtcac cgtcgcggcc gcggccttcc
     5581 aggtcgcgcg caacgcggcc caacgcagcc ttctgggcgg ggcggggccc tggggcgcga
     5641 ctctggttcg gttcctgttc ggcctgccat tcagcatcct gttctcgagc gtcgccctgc
     5701 tcctcctacc ggcccggctt gaggtgacgc ccgtcaccct ggcctgggcg gcgctcggcg
     5761 gggcgatgca gctgggggcc accgcagccc tgctgacggc catgcaccgc tcgagcttcg
     5821 ctctcggcac ggccttccag cagagcggcc tcccgttcgc ggcgctcctg gggctgctgt
     5881 tcttcggaga ccatctgagc gccggcggct ggggcggggt gctgctggcc agcgccgggt
     5941 tgctggtgct gtcctggccg cgtggcggcg aggcggcgcg cgactggacg gcggc
//
LOCUS       DAORNX010000147         5967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9868_length_5967_cov_8.635656, whole genome shotgun sequence.
ACCESSION   DAORNX010000147 DAORNX010000000
VERSION     DAORNX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9868_length_5967_cov_8.635656"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1707
                     /locus_tag="PLO65_07905"
                     /pseudo
     CDS             136..1707
                     /locus_tag="PLO65_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
     gene            1754..2374
                     /locus_tag="PLO65_07910"
     CDS             1754..2374
                     /locus_tag="PLO65_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRD28208.1"
                     /translation="MDIKPTRRALLIAAPALMLPVPALALTPADQGLVDKAVSYLQGL
                     PSARGRFVQTDPRGSVSQGVLYLQRPGRARFEYDAPSSLLVVSDGKVVSVADKRLKTV
                     NRYFLSQTPLKLFLADEIRLDRGVTVSRVTRLADGFEITAQDRAGKTRGQIVLTFADS
                     PMQLLGWTITDARNAETRVRLTSLVRAGDLDPGLFRAPFPRPATQR"
     gene            2610..3416
                     /locus_tag="PLO65_07915"
     CDS             2610..3416
                     /locus_tag="PLO65_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRD28209.1"
                     /translation="MRLRLATWNINSVRPRGDLIGRMVDEVAPDVLCLQEIKCQTGEF
                     PREMFEALGFTDFRVAGQKGWHGVAIASRLPITDAPPLDVCREGHARCVGAVVAGIEI
                     QNFYIPAGGDTPDPLANPKFAHKLDFFGRLTAEMRRRDPKAPLVVTGDLNVAPGEFDV
                     WNHRYMSKIVSHTPVEVEAMKALTDSLGFIDLTRAATPEPEKLASWWSYRAADFRASN
                     RGLRLDHILATPGLRQAAFHTGKAVSQIHDTVRGWDRPSDHAPVTADFMV"
     gene            complement(3417..3833)
                     /locus_tag="PLO65_07920"
     CDS             complement(3417..3833)
                     /locus_tag="PLO65_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD28210.1"
                     /translation="MKPSPILLDTCAAVWAAEPDGLSASAQAALAEADAAGVAVYVSP
                     ITAWELGMLVSRGRVALLEPPMRWFSGLLEAGVQLADLDLSTLVDSSFLPQSRLRDPA
                     DRIITATARAMGCRIMTRDRPILAYAAAGHVQAIAC"
     gene            complement(3830..4228)
                     /locus_tag="PLO65_07925"
     CDS             complement(3830..4228)
                     /locus_tag="PLO65_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28211.1"
                     /translation="MSKYAPLTTHLASLGVAEAPMTFAEIEAVTGHALPASKRYPAWW
                     SNNPSNNVMTRAWLAAGYQTERVDIAGERLVFRRISAASGAWPEVTPSAPVRDGTLLE
                     RIRARLGGTVTITPGADLTAPAGEIWDAER"
     gene            complement(4292..4978)
                     /locus_tag="PLO65_07930"
     CDS             complement(4292..4978)
                     /locus_tag="PLO65_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD28212.1"
                     /translation="MVQRKTLLIVDDDADLRGALAEQLQLHEEFAVVQAETAGEGARL
                     GKEARPDLILLDVDLPDMDGREACRLMRKGGVTSPIIMLTAAASDADTILGLDSGANG
                     YLTKPFRFGVLLAHIRSQLRSHEASEDAVFRIGPYEFRPASKLLVDDKGKKVRLTEKE
                     TNILKYLYRSGSKAVSREELLTEVWGYNAGVTTHTLETHVYRLRQKIEPDPANARLLL
                     TEAGGYRLQP"
     gene            complement(5069..5734)
                     /locus_tag="PLO65_07935"
     CDS             complement(5069..5734)
                     /locus_tag="PLO65_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRD28213.1"
                     /translation="MPSSEVAAARLSVLGRIADAARRAGRDPDDVTLVAVSKQQPDDR
                     IEAMLAAGQRIFGENRVQEAQGRWAGRREGLELRLIGPLQTNKVREAVALFDVIETVD
                     REKLARALAAEIARSGRAPRLYVQVNTGEEPQKAGVAPGAADAFLRLCRDLGLTIEGL
                     MCIPPEGEPPTAHFTLLGRIAARNGLHRLSMGMSADYELAVACGATSVRVGSALFGGR
                     MPR"
     gene            5725..>5967
                     /locus_tag="PLO65_07940"
     CDS             5725..>5967
                     /locus_tag="PLO65_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine monophosphate synthase"
                     /protein_id="HRD28214.1"
                     /translation="MTASDDLQRLSALAASLRPRFASEKPLPRLLFVTDPARTPDPGG
                     VAARLPPGAGVVYRPFGAPDALPRAVALRSIATRNGL"
BASE COUNT          939 a         2142 c         2007 g          879 t
ORIGIN      
        1 ccggccagcg tcgctacctc cgccggcaca gactcgagcg tctcctctcc cgccgcggcg
       61 cgcaaggcca gcgacagcgg cttgaggaat tcagcgtagg cgctgtcgtc gaacaagccg
      121 cccaaggccg cgccccgcaa cagcggccgc gaggtccgcg agcagcagaa aacctttgat
      181 ttcgtccagc ccggcggctt cgccctgccc gagctggcca tgctggccaa gccgaagccg
      241 cgggccggca acttcgacga ggaaggcctg cgccagaacg cccggctgct ggagagcgtg
      301 ctggcggagt tcggcgtgcg cggcgtcatc gaccagatcc gtccgggccc ggtcgtgacc
      361 ctctacgaat tggccccggc gccgggcgtc aagcacgcgc gggtggtggc cctgtcggac
      421 gacatcgccc gctcgatggg cgtggccgcc tgccgcgtct cggtggtcca gggccgcaac
      481 gccatcgccc tggaactacc caacgcccgg cgggagacgg tctatctgcg cgaccttctg
      541 gccagcgccg actacgagaa acagtcccag accctgccga tggcgctcgg cgagacgatc
      601 ggcggcgagc cctacatcgc cgacctggcc aagatgcccc acctgctgat cgccgggacc
      661 accggctcgg gcaagtcggt cggggtcaac gccatgatcc tgtcgatcct ctaccgcctg
      721 ccgcccgagc agtgccgctt catcatgatc gacccgaaga tgctcgagct gagcgtctac
      781 gacgggatcc cccacctgct ggcgccggtg gtgaccgact cgaagaaggc catcgtcgcg
      841 ctgaagtgga ccgtgcgcga gatggaggac cgctaccgcc gcatggccaa gatcggcgtg
      901 cggggcatcg ccgcctacaa cgagcgggcc agggaggcga tcgacaaggg cgagcacttc
      961 gagcgcaccg tccagacggg cttcgacgac gccggccggc cgatcttcga gagcgagaag
     1021 atccgccccg agcacatgcc ctatctggtc gtggtcatcg acgaggtggc cgacctgatg
     1081 atggtcgccg gcaaggatat cgagggcgcc gtgcagcggc tggcccagat ggcccgcgcc
     1141 gccggcatcc acctgatcat ggccacccag cggccgtcgg tggacgtgat caccggcacc
     1201 atcaaggcca acttcccgac ccgcatcagc ttccaggtca cctcgcgcat cgacagccgc
     1261 accatccttg gcgagcaggg cggcgagcag ctgctgggcc agggcgacat gctctacatg
     1321 gccggcggct cgggcggccg gctgatccgc ctgcacggcc cgttcgtcgc cgacgaggag
     1381 gtcgcggcgg tggccaggtt cctgcgggag cagggccagc cgcagtatct cgacgaagtc
     1441 accgccggcg ccgacgacga cgccgatgaa ggccagggcg gcctggcctt cggcgacgag
     1501 aacggggccg gcgacggcgg gctgtacgac aaggccgtgg ccgtggtcac ccgcgacggc
     1561 aaggcctcga ccagctacat ccagcggcga ctgcagatcg gctacaaccg cgccgcctcg
     1621 ctgatggaac ggatggaaca ggagggcgtt gtcggtcccg ccaatcacgc cggcaagcgc
     1681 gagatcctgg tggggcctcc cccgtaggga gtcctgccct gaaatcgccg ttccgccctg
     1741 ctagacggga atcatggaca tcaaaccgac ccgccgcgcc ctgctgatcg ccgccccggc
     1801 cctgatgttg ccggtcccgg cgctggcgct gacgccggcc gaccagggac tggtcgacaa
     1861 ggccgtcagc tacctgcagg gcctgccgtc ggcccgcggc cgcttcgtcc agaccgaccc
     1921 gcgcggctcg gtcagccagg gcgtgctgta cctgcagcgt ccgggccggg cccgctttga
     1981 atacgacgcc ccctccagcc tgctggtcgt gtccgacggc aaggtggtgt cggtggctga
     2041 caagcggctc aagaccgtca atcgctattt cctcagccag acaccgctga agctgtttct
     2101 cgccgacgag atccgcctcg atcgcggcgt gacggtcagc cgggtgacgc gcctggccga
     2161 cggtttcgag atcaccgccc aggaccgggc cggcaagacc cgtggacaga tcgtcctgac
     2221 cttcgccgac agtccgatgc agcttctcgg ctggacgatc accgacgccc gcaacgccga
     2281 gacgcgcgtc aggctgacca gcctggtgcg ggccggcgat ctcgacccgg gcctgttccg
     2341 cgcgcccttc ccgcggccgg cgacgcagcg ctgatcgatt gtgacacatt cgcaacaggc
     2401 aactgacttg cgattcccga ttgacgtttc ggtccacaag ttgcgataat atcactcatg
     2461 gcgattgcgc gcccgacctc gcgcagccgt gcgataccgt gccggaacct atcccctccc
     2521 ggcggcggca tgagagacca ccttttcacg cccgggcgct cgcgcgtccg ggcgtttttc
     2581 tttccgccga aacagcctat ctgccgtcca tgcgcctgcg tctcgccacc tggaacatca
     2641 actccgtccg cccgcgcggc gacctgatcg gccgcatggt cgacgaggtc gcgcccgacg
     2701 tcctgtgtct gcaggagatc aagtgccaga ccggcgagtt tccacgcgaa atgttcgagg
     2761 cgctgggctt caccgatttc cgcgtcgccg gccagaaggg ctggcacggc gtggcgatcg
     2821 cctcgcggtt gccgataacg gacgccccgc cgctcgacgt ctgtcgcgag ggccacgccc
     2881 gctgcgtcgg ggcggttgtc gccggcatcg agatccagaa cttctacatc ccggccggcg
     2941 gcgacacccc agacccgctg gcgaacccca agttcgccca caagctggac ttcttcggcc
     3001 gcctcaccgc cgagatgcgc cgccgcgacc cgaaggcgcc gctggtggtc accggcgacc
     3061 tcaacgtcgc gcccggcgag ttcgacgtct ggaaccaccg ctacatgtcg aagatcgtca
     3121 gccatacccc cgttgaggtc gaggccatga aggcgctgac cgactcgctg ggcttcatcg
     3181 acctgacccg cgcggcgacg ccggagccgg agaagctggc cagctggtgg agctaccgcg
     3241 cggccgactt ccgcgcctcc aaccgcggcc tgcgactgga ccacatcctg gccactcccg
     3301 ggctgcgcca ggccgccttc cacaccggca aggcggtcag ccagatccac gatacggtcc
     3361 ggggctggga ccggccgagc gatcacgccc cggtgacggc cgacttcatg gtctgatcag
     3421 caggcgatcg cctggacatg gccggcggcc gcataggcga ggatcggccg gtcgcgggtc
     3481 atgatccggc agcccatggc ccgggccgtg gcggtgatga tccggtcggc cggatcgcgc
     3541 agccgcgact ggggcaggaa cgaggagtcc accagcgtcg acaggtcgag atcggccagc
     3601 tggaccccgg cctcgagcag gccggaaaac cagcgcatcg gcggctccag cagcgccacc
     3661 cggccgcgtg acaccagcat gcccagctcc caggccgtga tcggcgagac gtagacggcg
     3721 acccccgccg cgtcggcctc ggcgagagcc gcctgggccg aggccgacag gccgtccggt
     3781 tcggccgccc agaccgccgc gcaggtgtcc agcaggatcg gcgagggctt caccgttcgg
     3841 catcccagat ctcgccggcc ggcgccgtca ggtcggcgcc cggcgtgatg gtcaccgtcc
     3901 cgcccagccg cgcgcggatt cgctccagca gcgtcccgtc gcggaccggc gcggaaggcg
     3961 taacctccgg ccaggccccg gaggccgcgg aaatccgccg aaacaccagc cgctcgcccg
     4021 cgatgtccac ccgttcggtc tggtagccgg ccgccagcca ggcccgggtc atgacattgt
     4081 tggacggatt gttgctccac caggccggat agcgcttcga tgccggcagg gcatggccgg
     4141 tgaccgcctc tatctcggca aaggtcatcg gcgcctcggc gacgcccagg gacgccagat
     4201 gggtcgtcaa aggggcgtat ttgctcatga cctggcctga atccggcaac tgtgtgcgga
     4261 gctagactag gtagacttag ttgtgtgtca actaaggttg cagacgatag ccaccggcct
     4321 cggtcaacag cagccgggcg ttggccgggt cgggctcgat cttctgccgc aaccggtaga
     4381 catgggtctc cagcgtgtgc gtggtgaccc cggcgttgta gccccacacc tcggtcagca
     4441 gctcttcacg ggaaaccgcc ttggatcccg agcgatagag gtacttgagg atgttggttt
     4501 ccttctcggt caaccggacc ttcttgccct tgtcgtcgac cagcagcttc gaggcgggcc
     4561 ggaactcata gggcccgatg cggaaaaccg cgtcctcgga ggcttcgtgg ctgcgcagct
     4621 ggctgcggat gtgggccagc agcacgccga agcggaaggg cttggtcaga tagccgttgg
     4681 ctccggaatc gagacccaga atggtgtccg cgtcgctggc cgcggcggtc agcatgatga
     4741 tcgggctggt caccccgccc ttgcgcatca gccggcaggc ctcacggcca tccatgtccg
     4801 gcaggtcgac gtcgagcaga atcaggtccg gccgggcttc cttgccgaga cgggcgccct
     4861 ccccggcggt ctcggcctgg acgacggcga attcctcgtg gagctggagc tgctcggcca
     4921 gggcgccgcg aagatcggcg tcgtcgtcga cgatcaggag ggtcttgcgt tgaaccatga
     4981 ggggaccatg caccgcttcg gcccctgagg ccaaccgccg gagggctttc accgcacaga
     5041 tagggtctcc ccgaggcgag cgcgaccctc agcgcggcat ccggccgcca aacagggccg
     5101 agccaaccct tacgctggtc gcgccgcagg ctaccgccag ctcgtagtcg gcgctcatgc
     5161 ccatggacag gcggtggagg ccgttgcggg cggcgatccg ccccagcagc gtgaagtgcg
     5221 ccgtcggcgg ctcgccctcc ggcgggatgc acatcagccc ctcgatggtc aggccaaggt
     5281 cacggcacag gcgcaggaag gcgtcggccg cgccgggcgc caccccggcc ttctgcggct
     5341 cctcgccggt attgacctgg acatagaggc gcggggcgcg gccggagcgc gcgatctcgg
     5401 cggccagggc ccgggcgagc ttctcccggt cgaccgtctc gatgacatca aacagcgcca
     5461 ccgcctcacg caccttgttg gtctgcaggg ggccgataag ccgcagctcg aggccctccc
     5521 ggcggccggc ccagcgcccc tgggcctcct gcacccggtt ctcgccgaat atccgctgtc
     5581 cggcggccag catggcctcg atccggtcgt caggctgttg cttggagacc gcgaccagag
     5641 tcacatcgtc agggtcacga ccggcgcgac gggcggcgtc ggcgatgcga cccaacacgg
     5701 aaaggcgcgc ggcggcgact tcggatgacg gcaagtgacg atctccagcg gctgtcggcc
     5761 cttgcggcgt ccttaaggcc ccgtttcgcc agcgaaaagc ccctgccgcg cctgctgttc
     5821 gtcaccgatc ccgcgcggac gcccgatccc ggcggggtgg cggcccggct gccgcctggc
     5881 gcgggggtcg tctaccggcc cttcggcgca ccggacgccc ttccccgggc cgtggccctg
     5941 cgatcgatcg cgacccgcaa cgggctg
//
LOCUS       DAORNX010000148         5945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_9909_length_5945_cov_6.912054, whole genome shotgun sequence.
ACCESSION   DAORNX010000148 DAORNX010000000
VERSION     DAORNX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_5945_cov_6.912054"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="flgA"
                     /locus_tag="PLO65_07945"
     CDS             <1..366
                     /gene="flgA"
                     /locus_tag="PLO65_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRD28215.1"
                     /translation="DMAWTKVAGAPADTPRDADAVIGMTARRPLREGAVVALRDVVAQ
                     QVIKPGDTVQVTWSDNGISLTLQAKALAAAAVGQSFNVQNPASKKIIEAVATAPGQAV
                     TGPDAQRLKAAGAAQYAAR"
     gene            379..1107
                     /gene="flgH"
                     /locus_tag="PLO65_07950"
     CDS             379..1107
                     /gene="flgH"
                     /locus_tag="PLO65_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRD28216.1"
                     /translation="MRLATLAVLAFAPLAACSTVKEAVSGPELAPIGYPAALVPQQQA
                     VLTPQTVSAQPASANSLWRAGARAFFVDQRASRVGDILTVQIDIDDSARTQNNTTTSR
                     TAGQTMGVPHLFGLESSLGKILPGGFDPSAMIETGSTSNKAGAGAVTRSEKISLTVAA
                     VVTGVLPNGNLIIQGTQEVMTNGELRRLTVSGIVRPEDISAANTVKHTQIAEARIAYG
                     GRGDISRVQRTPAGQAVVERFTPF"
     gene            1154..1615
                     /locus_tag="PLO65_07955"
     CDS             1154..1615
                     /locus_tag="PLO65_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28217.1"
                     /translation="MPRTVSILCAMAALALGSCVSRPVAPGFAWSYQNNAGEGPKLAY
                     GAPASDNIVLMMTCQPGSQRVDLSLLGGSPRAGLVLASGDARQRLAGDLVAAPGLGQM
                     IETSTHAASPPLARFARTGDLSLIDRGKTVRLDASPLERQGVSQFFEACAA"
     gene            complement(1625..3541)
                     /gene="dxs"
                     /locus_tag="PLO65_07960"
     CDS             complement(1625..3541)
                     /gene="dxs"
                     /locus_tag="PLO65_07960"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRD28218.1"
                     /translation="MPPVTPLLDKVSSPADIRGFSITDLKQLADEVRAETIDAVSVTG
                     GHLGAGLGVVELTVALHHVFETPRDIVIWDVGHQAYPHKILTGRRDRIRTLRMGGGLS
                     GFTKRAESEYDPFGAAHAATSISAALGFCAARDAKGETNKVVAVIGDGSMSAGMAYEA
                     MNNAAETTKQLIVILNDNDMSIAPPVGGMSAYLARMVSSGGYQSLRKLGKQVAGAFPK
                     PLQKALGKAEEYARGMVTGGTFFEELGFHYVGPIDGHDLDALTPVLKNARDFEGKPVL
                     VHVVTQKGKGYAPAENAADKYHGVVRFNVVTGEQAKAAAGPPGYTKVFAQALVKEAEK
                     DDRIVAVTAAMPSGTGLDIFGKAFPDRMFDVGIAEQHAVTFAAGIAADGMKPFAAIYS
                     TFLQRGYDQVVHDVAIQRLPVRFAMDRAGLVGADGPTHAGSFDIGFMGALPGMVLMAA
                     ADEVELARMVATAAAIDDRPSAFRYPRGEGLGLEMPASNEPLEIGRGRIVREGSAVAI
                     LSFGTRLSESLKAADLLAARGLTATVADARFAKPLDIELILRLAREHEALITVEEGAM
                     GGFGAFVLQALAEHGALDRGLKVRTLTLPDIFQDQDKPDLMYAQAGLDAEGIAAGALA
                     ALGVTNVTTAGRRA"
     gene            complement(3583..4464)
                     /locus_tag="PLO65_07965"
     CDS             complement(3583..4464)
                     /locus_tag="PLO65_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD28219.1"
                     /translation="MSLGRRIAETADVVTVALDALLPRADGPECRLTEAMRYAALGPG
                     KRLRPFFALETGKMFDLPERPVLRAACALECIHAYSLVHDDLPAMDDDDLRRGRPTVH
                     RQYDEATAILAGDALQTAAFEIMAHPDTHPDGNVRAELVRRLAHASGAQGMAGGQMID
                     LLGVREDLGGVARMQRLKTGALIAFAFEIPLIIAHAQDAERSALMAFAQDLGLAYQIV
                     DDLLDVEGDPENLGKAAGGKDAARGKTNYVTLLGIDAAKERVAHLAGQTRAHLDMFGE
                     RAATLRASVDFVLDRNS"
     gene            complement(4461..4715)
                     /locus_tag="PLO65_07970"
     CDS             complement(4461..4715)
                     /locus_tag="PLO65_07970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRD28220.1"
                     /translation="MTDAPAIDPAIAAMSFEEALAELEKIVQRLESGSAPLEESISIY
                     ERGAALKAHCEARLEQARLRVEKVVLGQGGPSGVEPAEFS"
     gene            complement(4770..5414)
                     /locus_tag="PLO65_07975"
     CDS             complement(4770..5414)
                     /locus_tag="PLO65_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD28221.1"
                     /translation="MVEVVAADGALTTSDTACPGAVILARHGEPDLSRRVLLNAEQYR
                     LWWGTYEGKGLRQGQTPPEALKRTASRAGAVIASTRPRSMETALAVCEGRAFARDPMF
                     IEAPLPPPPFPSWVRMSPRLWGFISRFWWWFFDHHGGEESRAEAEARADQAARLVADL
                     ARDGQDVLVVAHGFFNTMIGEALKRQGWRCTEDQGFRYWRARRFERIQDRRRIG"
     gene            complement(5473..>5945)
                     /locus_tag="PLO65_07980"
     CDS             complement(5473..>5945)
                     /locus_tag="PLO65_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRD28222.1"
                     /translation="GLARVAVVDFDVHHGNGTQAAFEHDPTLFLASIHQRPLYPGTGD
                     AGETGVGNIANGVVGPHAPRERWRKVFEGLMGQVDAFAPDLILVSAGFDAHVRDPLAS
                     QSLEAEDFAWATRAIVSVANGRAKGRVVSSLEGGYDLQALGRSALAHVQALQEG"
BASE COUNT          891 a         2131 c         2041 g          882 t
ORIGIN      
        1 gacatggcct ggaccaaggt cgccggcgct cccgccgaca cgccgcgcga cgccgacgcc
       61 gtcatcggca tgaccgcccg gcgtccgctg cgcgagggcg cggtcgtcgc cctgcgcgat
      121 gtcgtcgccc agcaggttat caagcccggc gacacagtcc aggtgacctg gtccgacaac
      181 ggcatcagcc tgaccctgca ggccaaggcg ctggccgccg ccgccgtcgg ccagagtttc
      241 aacgtccaga atcccgcctc gaagaaaatc atcgaggcgg tcgcgaccgc ccccggacag
      301 gccgtgaccg gtcccgatgc gcagcggctg aaggccgccg gcgccgccca gtacgccgcc
      361 cgctagagga ttcattccat gcgtctcgcc accctcgccg ttctcgcctt cgcgccgctc
      421 gccgcctgtt ccaccgtcaa ggaagcggtc agcgggccgg aactggcgcc gatcggctac
      481 cccgccgccc tcgtgcccca gcagcaggcc gtgctgacgc cccagacggt cagcgcccag
      541 ccggccagcg ccaattccct gtggcgggcc ggagcccggg ccttcttcgt cgaccagcgc
      601 gcctcgcgcg tcggcgacat cctgacggtg cagatcgaca tcgacgattc cgctcgcacg
      661 cagaacaaca ccaccacctc gcgcaccgcc ggccagacca tgggcgtgcc gcacctgttc
      721 gggctggaaa gctcgctggg caagatcctg ccgggcggct tcgacccctc ggcgatgatc
      781 gagaccggct cgaccagcaa caaggccggc gccggggcgg tgacccgctc ggagaagatc
      841 agcctgaccg tcgcggccgt cgtcaccggg gtcctgccca acggcaacct gatcatccag
      901 ggcacccagg aggtgatgac caacggcgaa ctgcgccggc tgaccgtctc gggcatcgtg
      961 cgtccggaag acatctcagc ggccaatacc gtcaagcaca cccagatcgc cgaggcccgc
     1021 atcgcctacg gcggccgggg cgacatcagc cgggtccagc gcacgccggc cggccaggcc
     1081 gtggtcgagc gcttcacccc cttctgaacc ggacggggcg ttgcgcgttt cctccagcgg
     1141 gaaggaggcg cgaatgcccc ggactgtgtc gattctctgc gcgatggcgg ccctcgccct
     1201 gggcagctgt gtttcacgcc cggtcgcgcc cgggttcgcc tggagctacc agaacaacgc
     1261 cggcgagggt ccgaagctgg cctacggcgc gccggcttcg gacaacatcg tgctgatgat
     1321 gacctgccag cccggctcgc aacgggtgga tctgtcgctg ctcggcggct cgccgcgggc
     1381 cggccttgtg ctggcgtcgg gcgacgcccg ccagagactg gccggcgact tggtcgccgc
     1441 gcccggactc gggcagatga tcgagacctc gacccatgcc gcctcgccgc ccctggcccg
     1501 cttcgcccgc accggcgacc tgtcgctgat cgaccgcggg aagacggtgc ggctggacgc
     1561 ctcgccgctc gagcgccagg gcgtcagcca gttcttcgaa gcctgcgccg cctagatttc
     1621 gggctcaggc ccgtctgccc gccgtggtca cattggtgac ccccagcgcc gccagggcgc
     1681 cggcggcgat gccctcggcg tcgaggccgg cctgggcgta catcaggtcc ggcttgtcct
     1741 ggtcctggaa gatgtccggc agggtcaggg tgcgcacctt cagcccgcgg tcgagcgcgc
     1801 cgtgctcggc gagcgcctgc agcacgaagg cgccgaagcc gcccatggcc ccctcctcca
     1861 cggtgatcag ggcttcgtgc tcgcgggcca gccgcaggat cagctcgatg tcgaggggct
     1921 tggcgaagcg ggcgtcggcg acggtcgcgg tcaggccgcg ggcggccagc aggtcggcgg
     1981 ccttgaggct ttccgaaagc cgcgtgccga atgacaggat ggccacggca gagccctcgc
     2041 ggacgatgcg gccccggccg atctccagcg gttcgttgct ggccggcatc tcgaggccca
     2101 gcccctcgcc acgcggatag cggaaggcgc tgggacggtc atcgatcgcc gccgcggtgg
     2161 ccaccatccg ggccagctcc acctcgtcgg cggcggccat caggaccatg cccggcaggg
     2221 cgcccatgaa gccgatgtcg aagctgcccg cgtgggtcgg accatcggcg ccgaccaggc
     2281 ccgcgcggtc catggcgaag cgcaccggca ggcgctggat ggccacgtcg tggaccacct
     2341 ggtcatagcc gcgctgcagg aaggtagagt aaatcgccgc gaacggcttc atgccgtcgg
     2401 ccgcgatgcc ggcggcgaag gtcaccgcgt gctgttcggc gatgccgacg tcgaacatgc
     2461 ggtcgggaaa ggccttgccg aagatgtcca ggccggtgcc ggacggcatg gcggcggtga
     2521 cggcgacgat acggtcgtcc ttctccgcct ccttgaccag cgcctgggcg aacaccttgg
     2581 tgtagccggg cggaccggcg gcggccttgg cctgctcgcc ggtgacgacg ttgaacctga
     2641 ccaccccgtg gtacttgtcg gccgcgttct cggccggggc gtagcccttc cccttctggg
     2701 tcacgacgtg aaccagcacc ggcttgccct cgaagtcccg ggcgttcttc agcaccgggg
     2761 tcagggcgtc caggtcgtgg ccgtcgatcg ggccgacgta gtggaagccg agctcctcga
     2821 agaaggtccc gccagtgacc atgccgcggg cgtattcctc ggccttgccg agggccttct
     2881 gcagcggctt ggggaaggcg ccggcgacct gtttgcccag cttgcgcagc gactggtagc
     2941 cgccgctcga gaccatgcgg gccagatagg cgctcatgcc gccgaccggc ggggcgatcg
     3001 acatgtcgtt gtcgttgagg atgacgatca gctgcttcgt ggtctcggcc gcgttgttca
     3061 tggcctcgta ggccatgccc gcgctcatcg agccgtcgcc gatgacggcc acgaccttgt
     3121 tggtctcccc cttcgcgtcg cgggcggcgc agaagcccag cgccgccgag atcgaggtcg
     3181 cggcatgggc cgcgccgaac gggtcgtatt cgctctcggc gcgcttggtg aagcccgaca
     3241 gcccgccgcc catgcgcagg gtgcggattc ggtcgcgccg gccagtgagg atcttgtgcg
     3301 gataggcctg gtggccgacg tcccagatga cgatgtcccg aggcgtttcg aacacgtggt
     3361 gcagggcgac ggtcagctcg acgacgccca gtccggcgcc cagatgtccg ccggtcaccg
     3421 agaccgcgtc aatggtttcg gcgcggactt cgtcggccag ttgcttcagg tcggtaattg
     3481 aaaagccgcg aatgtctgcg ggcgacgaaa ccttgtcgag cagcggcgtc acaggaggca
     3541 tgaaaatccc gtcgattacg aatggccgcg acgaaagcgc ggttacgagt tccggtccag
     3601 cacgaaatcc actgaagcgc gcagagttgc cgcccgttcg ccgaacatgt cgaggtgggc
     3661 gcgggtctgg ccggccagat gggccactct ttccttcgcc gcgtcgatcc ccagcagggt
     3721 tacatagttc gttttgcccc gcgccgcatc ctttccaccg gccgccttgc cgaggttctc
     3781 cggatcacct tcgacgtcca gcagatcgtc gacgatctgg taggccaggc cgagatcctg
     3841 tgcaaaagcc atcaaggccg atcgttccgc gtcctgggcg tgggcgatga tgagcgggat
     3901 ctcgaacgcg aacgcgatca gcgcgccggt cttcagccgc tgcatgcggg ccacccctcc
     3961 gaggtcctcg cgcacgccga gcaggtcgat catctggccg cccgccatgc cctgggcgcc
     4021 cgaggcgtgg gccagccggc gcaccagctc ggcccggaca ttgccgtccg gatgggtgtc
     4081 ggggtgggcc atgatctcga acgcggccgt ctgcagggcg tcgccggcga ggatggccgt
     4141 cgcctcgtca tactgacggt gcaccgtcgg ccggccgcga cgcaggtcgt catcgtccat
     4201 cgccggcagg tcgtcgtgca ccagactgta ggcgtggatg cattccaggg cgcaggccgc
     4261 gcgcagcacc ggccgctcgg gcagatcgaa catcttgccg gtctcaagcg cgaagaacgg
     4321 ccgcagccgc ttgcccggac cgagggccgc atagcgcatc gcctcggtca gccggcattc
     4381 ggggccgtcg gcccgcggca gcagtgcgtc gagcgccacg gtcaccacat cggcggtttc
     4441 ggcgatgcgc cggccgaggc tcacgagaac tcggccggct cgacgccgct cggcccaccc
     4501 tggcccagca ccaccttctc cacccgcagc cgcgcctgct ccagccgcgc ctcgcagtgg
     4561 gccttcagcg ccgcgccgcg ctcgtagatg gaaatcgact cctccagggg ggccgaccca
     4621 gattcgagcc gctggacgat cttttccagc tcggcgaggg cttcctcgaa gctcatggcg
     4681 gcgatggccg gatcgatggc gggcgcgtcg gtcatttcgt gttccaggag agtccggtcc
     4741 agcctagagg cgcgggaccg acggggcaat cagccgattc ggcggcgatc ctgaatccgt
     4801 tcgaagcgcc gggcgcgcca gtagcggaag ccctggtcct cggtgcagcg ccagccctgc
     4861 cgtttgaggg cctcgccgat catggtgttg aagaagccgt gggcgaccac cagcacgtcc
     4921 tgcccgtcgc gggccaggtc agcgaccagc cgggccgcct gatcggcgcg cgcctcggcc
     4981 tcggcgcgac tttcctcgcc gccatgatga tcgaagaacc accaccagaa acgggagatg
     5041 aagccccata gccggggcga catccgcacc caggacggaa acggcggcgg cggcagcggg
     5101 gcctcgatga acatcgggtc ccgggcgaag gcccggccct cgcagacggc cagggctgtc
     5161 tccatcgaac gggggcgggt cgaggcgatg acggcgccgg cccggctggc cgtccgtttc
     5221 agcgcttcag gcggcgtctg cccctggcgc agccccttgc cctcgtaggt cccccaccag
     5281 agacggtatt gctcggcgtt gaggagcacc cggcgcgaca ggtccggctc gccatggcgg
     5341 gcgagaatca ccgcgcccgg acaggcggtg tcagatgtcg tcaatgcacc gtcggcggcg
     5401 accacctcga ccatgtttcc ccggggctgg tgaaagacgc cgcacctgac gcggaccgtt
     5461 cgccggcgcc cgtcaaccct cctgcaacgc ctgcacatgc gccagggcgg aacgcccgag
     5521 cgcctgcaag tcgtagccac cctcgaggga cgacacaacc cggcccttcg cgcggccgtt
     5581 ggcgaccgaa acgatggcgc gggtggccca ggcgaaatcc tcggcctcca gcgactggct
     5641 ggccagcggg tcgcggacgt gggcgtcgaa ccctgccgag accaggatca ggtccggggc
     5701 gaaggcgtcg acctggccca tcaggccctc gaacaccttg cgccaccgct cccgcggcgc
     5761 atgcggccca accacgccgt tggcgatgtt accgacgccc gtctcgccgg cgtcgccggt
     5821 gcccggatag agcggtcgct ggtgaatcga ggccaggaac agggtcgggt cgtgttcgaa
     5881 ggccgcctgg gtgccattgc cgtggtggac gtcgaaatcg accaccgcca cccgcgccag
     5941 ccccg
//
LOCUS       DAORNX010000149         5898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10008_length_5898_cov_6.266130, whole genome shotgun sequence.
ACCESSION   DAORNX010000149 DAORNX010000000
VERSION     DAORNX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10008_length_5898_cov_6.266130"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLO65_07985"
     CDS             <1..631
                     /locus_tag="PLO65_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FlbA"
                     /protein_id="HRD28223.1"
                     /translation="GRLDLWAPMASVLRRFRRAVSAFPDAAGFLAPDPARVAYWRSQL
                     PAGPTVGLLWKSLIQAGARHRFYSPFDQWKPVLTVPGATFVNLQYGDCAGELEAARRE
                     LGVEIWTPPGIDLKQDLDEVAALCRALDLVVGPSNATSCLAGAAGVPLWLLSTPGAWP
                     MLGTDRYPWYPQARVFLPPTLGQWEPAMQAMGQALSQWLAAGTKAPGAG"
     gene            680..1105
                     /locus_tag="PLO65_07990"
     CDS             680..1105
                     /locus_tag="PLO65_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HRD28224.1"
                     /translation="MTPDMTFQSGSDAQDRAETLDETHLTKDGRSIANFDQIPDVFDA
                     TSADDDDDIDGYEDEEFDPDEVAATLQTARDDEGMDIPRDPAALAGGRSEDDRTDAPS
                     DPSEKVDEDADPHVDKQLDKGLKETFPASDPVSINPGAD"
     gene            1151..1297
                     /locus_tag="PLO65_07995"
     CDS             1151..1297
                     /locus_tag="PLO65_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28225.1"
                     /translation="MPDPGKPDAEDTVQTLDEALEEGLEETFPASDPPAALRREPAEP
                     PKPA"
     gene            1375..2247
                     /locus_tag="PLO65_08000"
     CDS             1375..2247
                     /locus_tag="PLO65_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRD28226.1"
                     /translation="MSSRFEGTSNYIATEDLKVAVNAAVALERPLLIKGEPGTGKTVL
                     AYEVAKAMGAPLITWHIKSTTKASQGLYEYDAVTRLRDSQLGEERVKDVRNYIKKGKL
                     WEAFESPVRPVLLIDEIDKADIEFPNDLLQELDRMEFYVYETDETVSAKVRPIIIITS
                     NNEKELPDAFLRRCFFHYIRFPEAEVMQEIVDVHFPGIKEKLVAEAMRIFYDMRKVPG
                     LKKKPSTSELLDWLKLLMVEDIDEAILREKDPTKLIPPLHGALLKNEQDVHLFERLAF
                     LARREGAGGSRPGQ"
     gene            2338..3105
                     /locus_tag="PLO65_08005"
     CDS             2338..3105
                     /locus_tag="PLO65_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28227.1"
                     /translation="MNRIALLAAGCAALALSACDHPDAARQRQARALKVISKLDCPET
                     QGHLTRTAQAADGLSCAYGAEGVEVTLQLVRLENGDAAGALSPIETELRGLMPRAAPD
                     AAEATAKAEADASPGEEVDIRLPGVTIKAGDAAANISVGGAEGAQINANDDGAEIRVQ
                     RNVEVNGKKVESSVRRRHKDDGVYARFILASDKATGGWSVVGYEARGPKGGPLVVATL
                     KAKRGHDADDDSFSDADALVRHNVGGRKSTGIVIGAD"
     gene            complement(3089..3463)
                     /locus_tag="PLO65_08010"
     CDS             complement(3089..3463)
                     /locus_tag="PLO65_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28228.1"
                     /translation="MTLSRIVMRLARNPGTEFADGDDHRGYSLVAPLQADGKLDVEGF
                     QAQRADCSVRRFAPDEEAVNGVLKRRGQTWYFDYDAADDTDDEPVHRLGEHRFAVGEY
                     VSIADEDGRMLAYKVTEVSPLR"
     gene            complement(3487..4299)
                     /locus_tag="PLO65_08015"
     CDS             complement(3487..4299)
                     /locus_tag="PLO65_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD28229.1"
                     /translation="MSWARIMAPLAGGAHDVDILKAAAAVAAPFKAEVAAVHAPADIA
                     DLMPWMGEGFMGGVQITAMDSLKEAAAEGERTARAAVEACGYANTRFMTLETPVWAGL
                     SMEGRLSDVVLFDTEAARGRGPLAEAFQQVVADEQRPTIVARPGIRADGVVVVAWDGG
                     KEASRAARTALPLLQKASRVVILAAPAASSRSFEPDRLKEFFGARGVAAEVELISESG
                     DAAHLLLAAAERLSASILVAGAFGHPRLQEYIFGGTTRTLLNNDGPSLFLSH"
     gene            4393..5064
                     /locus_tag="PLO65_08020"
     CDS             4393..5064
                     /locus_tag="PLO65_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HRD28230.1"
                     /translation="MIRNERVAKGLRGARSGNSTPRAWQRMLSGRRLDLLDPSPVDIE
                     IEDIAHGLARVARWNGQTIGEHGFSVAQHSIVVEELVAHIQPAVDPKWRLAALLHDAS
                     EYVIGDMISPFKAALGVSYKDFEERLEHAIHARFGLPAKTPVAIKKLIKQADRACAFF
                     EATQLAGFSHEEALAFFGAPPAGYRLTIEPQAPSEAQKRYVHRFHVLSEAAGFEPVQA
                     AFDTE"
     gene            5138..5377
                     /locus_tag="PLO65_08025"
     CDS             5138..5377
                     /locus_tag="PLO65_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HRD28231.1"
                     /translation="MTDIIYRIVPHDGGWAYTLDGVFSETFADRGAAEAAARRAAAEQ
                     ELPGETVGIRFEDETGAWREELSDGDDRPTTHVEG"
     gene            5501..>5898
                     /locus_tag="PLO65_08030"
     CDS             5501..>5898
                     /locus_tag="PLO65_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HRD28232.1"
                     /translation="MSQSVVIGLSAVIMAIRGGEAVVLTVRPHDAATDRVSTLAGLPF
                     GPFDPEAHRTFEIALRDFVTEQTRFDLGYVEQLYTFGNKGRDAPRAEIGAGAARVVSV
                     GYLALTPSAVDTDAPDSAWAPWTRFFPWED"
BASE COUNT          978 a         2096 c         1998 g          826 t
ORIGIN      
        1 tggccggctc gacctgtggg ccccgatggc ctcggtgctg cgccggttcc gccgcgcggt
       61 gtccgccttc cccgatgccg cgggcttcct ggcgcccgat ccggcgcggg tcgcctactg
      121 gcgctcgcag ctgccggccg ggccgacggt cggcctgctg tggaagagcc tgatccaggc
      181 cggggcccgc catcgcttct attccccgtt cgaccagtgg aagccggtgc tgaccgtgcc
      241 gggcgcgacc ttcgtcaacc tgcagtacgg cgactgcgct ggggagcttg aggccgcccg
      301 ccgcgagctg ggggtggaga tctggacgcc gcccggcatc gacctgaagc aggacctcga
      361 cgaggtggcc gccctgtgcc gcgcgctcga ccttgtcgtg ggcccgtcga acgccaccag
      421 ctgcctggcc ggcgcggccg gggttccgct gtggctgttg tcgacgcccg gcgcctggcc
      481 gatgctcggg accgaccgct atccctggta tccgcaggcg cgggtcttcc tgccgccgac
      541 gctgggccag tgggagccgg ccatgcaggc gatgggtcag gccctgtcgc aatggctggc
      601 ggcaggaacc aaggcccctg gcgcgggttg attccgggac cggcggggcg cgttcggccc
      661 cgcagccagg agccacccca tgacccccga catgaccttt caatccgggt ctgacgccca
      721 ggaccgggcg gagaccctgg acgagaccca cctgacgaag gacggccggt cgatcgccaa
      781 tttcgaccag atccccgacg tcttcgacgc caccagcgcg gacgacgacg atgacatcga
      841 cggctacgag gacgaggaat tcgaccccga cgaggtcgcc gcgacactgc agacggcgcg
      901 cgacgacgag ggcatggaca tccctcgcga tcccgcagcc ctggcgggtg gccgcagcga
      961 ggacgatcgg accgacgccc cgtccgatcc gtcggaaaag gtcgacgaag acgccgatcc
     1021 ccatgtcgac aagcagctcg acaaggggct caaggagacc ttccccgcca gcgacccggt
     1081 gtcgatcaac cccggcgccg attgagaccg cgaacgcggg cgcctacctg aggtgcgaca
     1141 ggaggcgccc atgcccgatc ccggaaaacc cgacgctgaa gacaccgtcc agacgctgga
     1201 cgaagcgctc gaggaagggc ttgaggagac ctttccggca agcgatccgc ccgcggccct
     1261 tcggcgcgag ccggccgagc cgccgaaacc ggcttgaatg tcgcgatttg cgctttaggc
     1321 tgcccctgaa ttgaacgttt gttcgaatgc gcgaaaacgc gcccagggga tgtcatgagc
     1381 tcgcgttttg aaggcacgtc caactacatc gccaccgagg acctgaaggt cgcggtgaac
     1441 gccgccgtgg cgctggaacg gcccctgctg atcaagggcg aacccggcac cggcaagacg
     1501 gtgctggcct atgaagtggc caaggcgatg ggcgccccgc tgatcacctg gcacatcaag
     1561 tcgaccacca aggccagcca gggcctgtac gaatacgacg ccgtcacccg cctgcgcgac
     1621 agccagctgg gcgaggagcg ggtcaaggac gtccgcaact acatcaagaa gggcaagctc
     1681 tgggaggcct tcgagagccc cgtgcgcccc gtgctgctga tcgacgagat cgacaaggcc
     1741 gacatcgagt tccccaacga cctcctgcag gagctcgacc ggatggaatt ctatgtctac
     1801 gagaccgacg agacggtctc ggccaaggtc cgcccgatca tcatcatcac ctcgaacaac
     1861 gagaaggaac tgccggacgc cttcctgcgc cgctgcttct tccactacat ccgcttcccc
     1921 gaggccgagg tgatgcagga gatcgtcgac gtccacttcc ccggcatcaa ggagaagctg
     1981 gtcgccgagg cgatgcgcat cttctacgac atgcgcaagg tgccgggcct gaagaagaag
     2041 ccctccacca gcgaactgct cgactggctc aagctgctga tggtcgagga catcgacgag
     2101 gcgatcctgc gcgagaagga tccgaccaag ctgatcccgc cgttgcacgg ggccctgctg
     2161 aagaacgaac aggacgtgca cctgttcgag cgcctcgctt tcctggcgcg gcgtgaaggc
     2221 gccggcggct cgcgtccggg acagtagcgg cccccgccgc cttaccgcga tttcactgga
     2281 ccgttccgcc gtcgcgccgc acccttgcat tgcaagagag cacacgaggg acaccccatg
     2341 aaccgcatcg ccctgctggc cgccggctgc gccgccctgg ccctgtccgc ctgcgaccat
     2401 ccggacgccg cacgccagcg acaggcccgg gccctgaagg tgatcagcaa gctggactgc
     2461 cccgagacgc agggccatct gacgcgcacc gcccaggcgg ccgacggcct ctcctgcgcc
     2521 tacggcgccg agggcgtcga ggtgacgctg cagctggtca ggctggagaa cggcgacgcg
     2581 gccggggccc tgtcgccgat cgagaccgaa ctgcgcggcc tgatgccgcg cgccgcgccg
     2641 gacgccgctg aagcaaccgc caaggccgag gctgacgcca gcccgggcga ggaagtcgac
     2701 atccgcctgc cgggcgtcac catcaaggcc ggcgacgcgg ccgccaacat cagcgtcggc
     2761 ggcgccgagg gcgcccagat caacgccaac gacgacggcg ccgagatccg cgtccagcgc
     2821 aacgtcgagg tcaacggcaa gaaggtcgaa tcctccgtcc gccgccgcca caaggacgac
     2881 ggcgtctacg cccggttcat cctggccagc gacaaggcca cgggcggatg gtcggtggtc
     2941 ggctacgaag cccgcgggcc caagggcggg ccgctggtcg tcgccaccct caaggccaag
     3001 cgcggccacg acgccgacga cgacagcttc tccgacgccg acgccctggt gcgccacaat
     3061 gtcggcggcc ggaagtcgac cggaatagtt atcggagcgg actgacctcg gtgaccttgt
     3121 aggccagcat gcggccgtcc tcatcggcga tggagacgta ctcgccgacc gcgaacctgt
     3181 gctcgcccag gcgatgtacg ggctcgtcgt cggtgtcgtc ggcggcgtcg tagtcaaaat
     3241 accaggtctg gccccgccgc ttgagcacac cgttgacggc ctcctcgtcg ggcgcgaacc
     3301 ggcgcacgga acagtcggcg cgctgggcct ggaagccctc gacatcaagc ttgccatcgg
     3361 cctgcagggg cgcgaccagg ctgtagccgc gatggtcgtc gccatcggcg aactcggtgc
     3421 cgggattgcg cgcaaggcgc atgacgatgc gcgaaagcgt cattgaggtc ctcgttttct
     3481 gtcaggtcag tgcgaaagga acagcgacgg tccgtcgttg ttcagcagcg tccgggtggt
     3541 gccgccgaag atgtattcct gcagccgcgg gtggccgaag gcgccggcca ccaggatact
     3601 ggccgacagg cgctcggccg cggccagcag cagatgcgcc gcgtcgcccg actcactgat
     3661 cagctcgacc tcggccgcca ccccgcgggc gccgaagaac tccttcagcc ggtcgggctc
     3721 gaagctgcgc gaggaagcgg ccggcgcggc gagaatcacc acccggctgg ccttctgcag
     3781 cagcggcagg gccgtgcggg cggcgcggct ggcctccttg ccgccgtccc aggcgaccac
     3841 cacgaccccg tcggcgcgga ttccgggccg ggcgacgatg gtcggacgtt gctcgtcggc
     3901 gacgacctgc tggaaggcct cggccagcgg cccgcggccg cgggcggcct cggtgtcgaa
     3961 cagcaccacg tcggacaggc gcccctccat ggacaggccg gcccagaccg gcgtctccag
     4021 ggtcatgaag cgggtattgg cgtagccgca agcctccaca gcggcgcggg cggtccgctc
     4081 accctcggcg gcggcctcct tgaggctgtc catggcggtg atctgcaccc cgcccatgaa
     4141 gccctcgccc atccacggca tcaggtcggc gatgtcggcg ggcgcgtgca cggcggcgac
     4201 ctcggccttg aacggcgccg ccacggcggc ggccgccttc aggatgtcca cgtcgtgcgc
     4261 cccgcccgca agcggcgcca tgatcctcgc ccagctcatc gaatgccctc cgttcaaacc
     4321 gaaccgtgat ctcaacacca cgcgcaaaca ttgatcttta tcaaccaatg cgccagtgga
     4381 agcgccctgc tgatgataag gaacgaacgg gtggcgaaag ggctgcgagg cgcgcgctcc
     4441 gggaatagca cgccgcgggc gtggcagaga atgctgtccg gccgacggct cgacctgctc
     4501 gacccctcgc ccgtcgatat cgagatcgag gacatcgccc acggcctggc ccgcgtggcg
     4561 cgctggaacg gccagaccat cggcgagcac ggcttctccg tcgcgcagca ttcgatcgtc
     4621 gtcgaggagc tggtggcgca catccagccg gccgtcgacc cgaagtggcg gctggcggcg
     4681 ctgctgcatg acgcctcgga gtacgtgatc ggcgacatga tcagcccgtt caaggcggcg
     4741 ctcggcgtca gctacaagga tttcgaggag cggctcgagc acgccatcca cgcccgcttc
     4801 ggcctgccgg cgaagacgcc cgtggcgatc aagaaactga tcaagcaggc cgaccgggcc
     4861 tgcgccttct tcgaggccac gcagctggcc ggcttcagcc acgaggaggc cctggccttc
     4921 ttcggcgcgc cgccggccgg ctatcgcctg accatcgaac cccaggcgcc gagcgaggcg
     4981 cagaagcgct acgtgcatcg cttccatgtg ctgtcggaag cggccgggtt cgagcccgtg
     5041 caggcggcgt tcgacaccga atagccggtt cggggcgctc cccgggcaac gggggccgcg
     5101 ccggcgcgtt gcagcagcag ccgcgcagga gccgaccatg accgacatca tctatcgcat
     5161 cgtcccccat gacggcggtt gggcctacac gctcgacggg gtgttttccg agaccttcgc
     5221 cgaccgcggc gcggccgagg ccgcggctcg tcgcgcggcg gccgagcagg aactgcccgg
     5281 cgagactgtc ggcatccgct tcgaggacga gaccggcgcc tggcgggagg agctgtccga
     5341 cggcgatgac cggccgacga cgcatgtgga gggataggga ctagagggac cggcgggatc
     5401 tgcgggaggc cgggtgacag cgatcagtcg acgcgaacac cgcgttcgac tactccctac
     5461 tcccctgatc ctgccccctg ccaccgtcct gccctcgccc atgtcccaat ccgtcgtcat
     5521 cggcctgtcc gccgtcatca tggccattcg cggcggcgag gcggtggtgc tgacggtgcg
     5581 cccgcacgac gccgcgaccg accgggtgtc gaccctcgcc ggcctgccgt tcgggccgtt
     5641 cgatccggag gcgcaccgca ccttcgagat cgccctgcgc gatttcgtca ccgaacagac
     5701 ccgcttcgac ctgggctatg tcgagcagct gtacaccttc ggcaacaagg gccgcgacgc
     5761 gccgcgggcc gagatcggcg ccggcgccgc gcgggtcgtg tcggtcggct acctggccct
     5821 gacgccctcg gccgtcgata cggacgcgcc ggacagcgcc tgggcgccct ggacgcggtt
     5881 ctttccgtgg gaagactg
//
LOCUS       DAORNX010000150         5859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10091_length_5859_cov_7.594935, whole genome shotgun sequence.
ACCESSION   DAORNX010000150 DAORNX010000000
VERSION     DAORNX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_5859_cov_7.594935"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLO65_08035"
     CDS             complement(<1..1390)
                     /locus_tag="PLO65_08035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD28233.1"
                     /translation="MAEDLDAIIIGAGFGGLTAAIKLKEAGFGRLVVLEKGDRIGGTW
                     RENTYPGACCDVPSRLYSLSFALNPDWTRAFSPQAEIRDYMERVAAQFGVVDRFRFNS
                     EVEKAVWDEAAGRWTVTVKGGETLSAPVLVSGLGQLNKPGYAGIPGRDRFKGDSWHSA
                     AWRHDIDLAGKTVGCIGAGASAIQYIPEIAKTAGKVIIFQRTPNYIVPRMDRAFSGFE
                     KWLYRTFPAIDRLNRWFIWQMMDLRFNAFKQGTKSAENFRKLALKYLDAVVTDPKLKA
                     KLTPDYPIGCKRILVSDDYYQTLVRPNVELVTEAVAEVTPGGVKTADGAEHACDVLVY
                     GTGFITTDFVSPVEIVGAGGRSLSAAWKDGAEAYLGVTVAGFPNLFMLYGPNTNLGHN
                     SIIVMIEAQVGYLVEAMKELKRRSARSLTVKDEAFKRFNVELQQDLTKTAWAGSCSSW
                     YKTASGKITNNWS"
     gene            1589..3619
                     /locus_tag="PLO65_08040"
     CDS             1589..3619
                     /locus_tag="PLO65_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRD28234.1"
                     /translation="MSSRKSGAVRAIGLVGPNGAGKTTLFEALLHAAGATDRQGVVGQ
                     AGAVGDGSPEAKQAGQSVEMNLAGFAFMDDHYALIDFPGSVEFRAECDLGLAALDLAI
                     VVIDPDPDKAVLIQPWLRELERLGIPRAFFVNKIDQARGHVDELLKALQPVSAIPLVA
                     RQLPIWKDDHVAGFIDLALERAFVYRAGKPSEQVEIPADLIDEEAAARFHMLEQMADF
                     DDGLMEILLEDGTPSPDTIFADLIAETRAAQIAPVFFGIAQQGNGVRRLLKALRHDAP
                     GPEAAAARLGLAGPGAYALKASHAGQAGKMVLARVFGSPLADGAELTLPDGARARVSG
                     LFSVQGQTTKKVSVVDVGDVVALGKLEGAHAGDLLSAGKTAMSARVAPAPRSPVFELA
                     IATRDRKDDVRLSGALQKLLEEDPGLRLSQDAALHEMRLGGQGETHLRVALDRLKRRY
                     QVEVVTHTPATPYRETIRKAVTQRGRHKKQSGGHGQFGDTLLEIRPLPRGEGFQFNDR
                     ITGGVVPKQWIPAVEQGVRDAMERGPLGFPVVDVAVTLIDGSFHAVDSSEMAFRTAGR
                     IGMQEGLAQAQPILLEPIEKLIIHAPNAATARITSSIANRRGRMLGFDARPGWPGWDS
                     IEVYMPHDERQDFILDLRSMTQGLGAFETAFDHMVELTGRKAEEVSQKSQAA"
     gene            complement(3650..4372)
                     /locus_tag="PLO65_08045"
     CDS             complement(3650..4372)
                     /locus_tag="PLO65_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD28235.1"
                     /translation="MTSPLPLKNRLLAALPPGEFALLSPQLMLVDLEKGRLICDPGDP
                     VETIYFPHDCVISLMTLMESGAAIESATIGREGAFGLPAALAPRQAMSRAIVQVPGRA
                     SRIAATALAEVCRRAPTLLTLIDRHGDALFGHAIQSVACNALHGVEARFCRWLLTCRD
                     RIDGDTVSLTQEFLADMLGVQRTTVTAIAGALQSRGLIRYRRGLVDILDRAGLEACAC
                     ECYDAVRQNYDRLLPEPRRARG"
     gene            4599..5645
                     /locus_tag="PLO65_08050"
     CDS             4599..5645
                     /locus_tag="PLO65_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD28236.1"
                     /translation="MKYRQLGRTGLYVSEICLGAMTFASEAAGMWGAIGAVDQRGVDD
                     MIGRALASGVNFIDTADVYSAGQSEQMVGAALRNLGVKRSDVVIATKCFGEMGRGPND
                     KGASRGHIMDAVKASLDRMQLDYIDLYQIHANDPVTPVEETLRALDDLVSQGLVRYAG
                     CSNWPAWKMMKAHGIADARGYARFETVQAYYSIAGRDLEREIVPMMQDQGMGLMVWSP
                     MAGGLLSGKFTRDGAGPNGARRVSFDFPPVNRDRAWDCVDAMKIIADARGVSVARIAL
                     AYVLAKPFVTTVIIGAKTVEQLDDNLAAAEIELTADEMAALDKASALPPEYPGWMLER
                     QGGRRVPEPFVKKG"
BASE COUNT          955 a         1956 c         2019 g          929 t
ORIGIN      
        1 tcgaccagtt gttggtgatc ttgcccgagg cggtcttgta ccagctggag caggacccgg
       61 cccaggcggt ctttgtcagg tcctgctgta gttcaacgtt gaaccgtttg aacgcttcat
      121 ccttcacggt cagcgatcgt gcgctgcggc gcttcagttc cttcatcgcc tcgaccagat
      181 agccgacctg ggcttcgatc atgacgatga tcgagttgtg gccgaggttg gtgttggggc
      241 catagagcat gaacaggttc gggaagcctg cgacggtgac gcccagatag gcctcggccc
      301 cgtccttcca ggccgcgctc aggctcctgc cgccggcgcc gacgatctcg accggcgaga
      361 cgaagtcggt ggtgatgaag ccggtgccgt agaccagcac gtcgcaggca tgctcggccc
      421 cgtcggccgt cttcaccccg cccggggtga cctccgcgac agcctcggtg accagctcga
      481 cattgggccg caccagggtc tggtagtagt cgtccgagac caggatgcgc ttgcagccga
      541 tcggatagtc gggcgtcagc ttggccttca gcttcgggtc ggtgaccacc gcgtcgaggt
      601 atttcagcgc cagcttgcgg aagttctcgg ctgacttcgt gccctgtttg aacgcgttga
      661 agcgcaggtc catcatctgc cagatgaacc agcgattgag ccggtcgatg gccgggaagg
      721 tccggtagag ccacttctcg aagccgctga acgcgcggtc catacgcggc acgatgtagt
      781 tgggcgttcg ctggaagatg atcaccttgc cggcggtctt ggcgatctcg gggatgtact
      841 ggatggcgct ggccccggcg ccgatgcagc cgacggtctt gccggccagg tcgatgtcgt
      901 gccgccaggc cgccgagtgc cagctgtcgc ccttgaacct gtcccggccg gggatgccgg
      961 cgtagccggg cttgttcagc tggcccaggc ccgacaccag caccggcgcc gacagggtct
     1021 cgccgccctt gacggtcacc gtccagcgcc cggcggcttc gtcccagaca gccttctcga
     1081 cctcgctgtt gaaccggaag cggtcgacga cgccgaactg cgcggccacg cgctccatgt
     1141 agtcgcggat ttcggcctgg ggcgagaagg cgcgggtcca gtcgggattg agcgcgaagg
     1201 acagggaata gagccgcgag ggcacgtcgc agcaggcgcc gggataggtg ttctcgcgcc
     1261 aggtgccgcc gatccggtcg cccttctcaa gcaccacgag gcgtccgaac ccggcttcct
     1321 tcagcttgat cgccgcggtc agtccgccga agcccgcccc gatgatgatg gcgtccagat
     1381 cctcggccat gccgccgcgt cctccctgct ttcacaaagt attgcgcagg ggcagggcgg
     1441 gggaaagcct gacccggggt cactttggct tgccctcgtc ccgcgccggg tgttcgatcg
     1501 gctcgtgggg cccgggtctc gcgccccctg aacctgaaac gacgcggcga ggccgcgcgt
     1561 ctcgccgccg aacggtggga gaatgcgcat gtcctcaagg aaatcaggcg cggttcgggc
     1621 gatcggtctg gtcggcccga acggcgccgg taaaacgacc ctgttcgagg cgctgctgca
     1681 cgcggccgga gccaccgacc gccagggtgt tgtcgggcag gccggggcgg tcggcgacgg
     1741 ctcgccggag gcgaaacagg ccggccagtc ggtcgagatg aacctggccg gcttcgcctt
     1801 catggacgac cactatgcgt tgatcgactt tccgggatcg gtcgaatttc gcgccgagtg
     1861 cgacctcggc ctggcggcgc tggacctggc gatcgtggtc atcgatcccg atccggacaa
     1921 ggccgtgcta atccagccct ggctgcggga actcgaacgc ctcgggattc cgcgcgcgtt
     1981 cttcgtcaac aagatcgatc aggcccgggg ccatgtggac gaactgctca aggcgttgca
     2041 gccggtcagc gccatcccgc tcgtcgcgcg acagttgccg atctggaagg acgaccatgt
     2101 cgcgggattc atcgacctgg cgctcgaacg ggccttcgtc taccgcgcgg gcaagccgtc
     2161 cgagcaggtc gagattccgg ccgacctgat cgacgaggag gcggccgccc gcttccacat
     2221 gctcgaacag atggccgact tcgacgacgg cctgatggaa atcctgcttg aggacggcac
     2281 gcccagcccc gacaccatct tcgccgacct gatcgccgag acgcgcgccg cccagatcgc
     2341 gccggtattc ttcggtatcg cgcagcaggg caacggcgtg cgccgcctgc tcaaggccct
     2401 gcgccacgac gcgcccgggc cggaggccgc ggccgcccgc ctgggactgg ccggacccgg
     2461 cgcctacgcc ctgaaggcct cgcacgcggg ccaggcaggc aagatggtct tggcgcgcgt
     2521 gtttggcagc ccgctggccg acggcgccga actgaccctg cctgacggcg cccgcgcccg
     2581 ggtcagcggc ctgttcagtg tccagggcca gacgacgaag aaggtcagcg tggtcgatgt
     2641 cggcgacgtc gtggcgctcg gcaagctgga aggcgcccac gctggcgacc ttctgtcggc
     2701 gggcaagacg gcgatgtcgg cccgggtcgc gcccgcgccg cgttcgccgg tgttcgaact
     2761 ggccatcgcc acccgtgacc gcaaggacga cgtgcgactg tcaggcgcgc tgcagaagct
     2821 gcttgaggag gatcccggcc ttcgcctgtc gcaggatgcg gccctccacg agatgcgcct
     2881 gggcggccag ggtgagaccc acctgcgcgt cgccctcgac cggctgaagc ggcggtacca
     2941 ggtcgaggtg gtgacccata ccccggccac gccctaccgc gagacgatcc gcaaggcggt
     3001 gacccagcga ggccggcaca agaagcagtc cggcggccac ggccagttcg gcgacaccct
     3061 gctggagatc cggccgctgc cgcgcggcga gggtttccag ttcaacgacc ggatcaccgg
     3121 cggcgtggtg cccaagcagt ggatcccggc cgtcgagcag ggcgtgcgcg acgccatgga
     3181 gcggggcccg ctcgggttcc cggtcgtgga tgtcgccgtg accctgatcg acggttcgtt
     3241 ccacgccgtc gactcgtcgg aaatggcctt ccgcacggcc ggccgcatcg gcatgcagga
     3301 ggggctggcc caggcccagc cgatcctgct cgagccgatc gagaagctga tcatccatgc
     3361 gcccaacgcg gcgaccgcgc ggatcacctc ctcgatcgcc aaccggcgag ggcggatgct
     3421 cggtttcgac gcccggcccg gctggcccgg ctgggacagc atcgaggtct acatgccgca
     3481 tgacgagcgg caggacttta tcctcgatct gcgctccatg acccaggggc tgggcgcgtt
     3541 cgagaccgcc ttcgaccaca tggttgaact gaccggccgc aaggccgagg aggtcagcca
     3601 gaaatcccag gcggcctgag cgtccggtgc cgtccgccgc gtccggtggt cagccccggg
     3661 cgcggcgggg ttcgggcagc aaccggtcgt agttctgtcg caccgcgtcg tagcattcgc
     3721 aggcgcaggc ctcgagcccg gcgcgatcca ggatgtcgac cagtccgcga cggtagcgga
     3781 tcaatccgcg cgattgcagc gcgccggcga tggccgtcac ggtggtgcgc tggacaccga
     3841 gcatgtcggc gagaaattcc tgcgtcaggc tgacggtatc gccatcgatc cggtcgcggc
     3901 aggtcagcag ccagcggcag aagcgcgcct cgacgccgtg cagggcgttg caggccacgg
     3961 actggatggc gtgaccgaac agcgcgtcgc cgtgccggtc gatcagggtc agcagggtcg
     4021 gtgcgcgccg gcagacctcg gccagggccg tcgcggcgat gcgggaggcg cgtcccggaa
     4081 cctggacgat ggcccgggac atggcctggc gcggggccag ggcggccggc aggccgaacg
     4141 cgccttcgcg gccgatggtc gccgactcga tggccgcccc gctctccatc aacgtcatca
     4201 gcgagatgac gcagtcatgg gggaagtata tggtctccac cggatcgccc ggatcgcaga
     4261 tcaggcggcc cttttccaga tcgaccagca tcagctgcgg cgacagcagg gcgaattccc
     4321 cgggcgggag ggcggccagc agccggttct tcaagggcag agggcttgtc atcacgactc
     4381 agaacgcaac gctacatcgg gggttgcaag ctataggtcg aggattcaaa gcactgtcgt
     4441 ttagccgaca taatcaagac atgattatat ctggcatcaa ttaaacaggc cgttgaagta
     4501 acagcgataa acggccaatg aaatctcacc tatgtcggaa tcccgacatt gaagacaatt
     4561 tcaaagcccg aaggggcgga gacaacggga ggatcgtcat gaaatacagg caattgggtc
     4621 ggacgggtct ctacgtctcc gagatctgtc tcggcgccat gaccttcgcc agcgaggccg
     4681 cgggtatgtg gggcgctatc ggcgcggtcg accagcgggg cgtcgacgac atgatcggcc
     4741 gggccctggc cagcggggtc aacttcatcg acaccgccga cgtctattcg gccggacagt
     4801 ccgagcagat ggtcggcgcg gcgctgcgca acctcggggt caagcgcagc gacgtggtca
     4861 tcgcgaccaa atgcttcggc gagatgggcc gggggccaaa tgacaagggc gcctcgcgcg
     4921 gccacatcat ggacgcggtc aaggccagcc tcgaccggat gcagctggac tacatcgacc
     4981 tctaccagat ccacgccaat gatccggtga ccccggtcga ggagaccctt cgggcgctgg
     5041 acgacctcgt gtcgcagggc ctggtccgct acgccggctg ctccaactgg ccggcctgga
     5101 agatgatgaa agcccacggc atcgccgacg cccgcggcta cgcccggttc gagacggtcc
     5161 aggcctacta ctcgatcgcc ggtcgcgacc tcgagcgcga gatcgtgccg atgatgcagg
     5221 accagggcat ggggctgatg gtctggtcgc caatggccgg cggtctgctg tcgggcaagt
     5281 tcacccgcga cggggccggc cccaacggcg cgcgccgggt cagcttcgac ttcccgccgg
     5341 tcaaccgtga tcgcgcctgg gactgcgttg acgccatgaa gattatcgcc gacgcccgcg
     5401 gcgtctcggt ggcccgtatc gccctcgcct atgtcctggc caagccgttt gtcaccacgg
     5461 tgatcatcgg ggccaaaacg gtcgagcagt tggacgacaa cctcgccgcc gccgaaatcg
     5521 agctcaccgc cgacgaaatg gccgcgctcg acaaggcctc ggccctgccg cccgagtacc
     5581 ccggctggat gctggagcgc cagggcggcc gccgcgtccc tgagccgttc gtgaagaagg
     5641 gctaggcgac ccgggagcag cgccgggtcc cgaccagact tgtcccgggc catgaggcgg
     5701 gcgcacctgc gccccctcgc aacaggagat acggatttgt cgcgatggcg tcacgccttg
     5761 ccctcgctcc tgcttgcggt cgccgtcgcc ggcgcgggct gtggccaggg cggcgcgccg
     5821 tccgacagga tcgcggcccg gtcagcggac ggggcgcgg
//
LOCUS       DAORNX010000151         5855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10100_length_5855_cov_6.906724, whole genome shotgun sequence.
ACCESSION   DAORNX010000151 DAORNX010000000
VERSION     DAORNX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10100_length_5855_cov_6.906724"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>225)
                     /locus_tag="PLO65_08055"
     CDS             complement(<1..>225)
                     /locus_tag="PLO65_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD28237.1"
                     /translation="ALLVLCQTHYRSGAVRDMAALTARAQAAGALVLWDLSHSTGVLE
                     VDLNGCNADLAIGCGYKYLNGGPGAPAFLFV"
     assembly_gap    226..386
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..703)
                     /locus_tag="PLO65_08060"
     CDS             complement(<387..703)
                     /locus_tag="PLO65_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28238.1"
                     /translation="MDSLDPVALDAADRLAGFRDAFARPGGLVYLAGNSLGPPPREAA
                     SRVARMVEREWGESLVRGWNSHDWFGAPARVGGKIAGLIGAHPDEVLVADSTSANLYK
                     LAVA"
     gene            882..1964
                     /locus_tag="PLO65_08065"
     CDS             882..1964
                     /locus_tag="PLO65_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD28239.1"
                     /translation="MQIKRSYLIAGLIVAAFLLFFIGGTVLRALAPKDRPAETAAAKA
                     SAAKADEPLVKIAVINEVQRPYVVSVRGRTDANRTVIVRAETAGPVAATPAREGSFVA
                     KGAILCQLSVDARQATLDQARAALKSRQLQREASERLAAQGYRSQTQVLQDQANLDAA
                     SASVRQAEVALEQVNVRAPFAGVFDSRDAEVGSYLAPGAPCGTMIQLDPLVIVADVSE
                     AEVGSIRVGAAARAMLVSGESLTGRVRSVGRNADPATRTYRVEIEARNPGLRVRSGLS
                     ADVKITVGAGPAMLVPAATLVLDSAGRQGVRFVGQDERVGFANVQVQDETAEGIWITG
                     LRGPARVIVAGQSYVSEGQKVRASAR"
     gene            1996..5247
                     /locus_tag="PLO65_08070"
     CDS             1996..5247
                     /locus_tag="PLO65_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD28240.1"
                     /translation="MIGALIDGAIKRRKVVLGIAAIASLFGLFAYMNLPRESSPNIPV
                     PFIAITVPYPGVSPEDSERLLVRPLERELQSLEGLKEMNAVAQQNAATVVLEFEVNFD
                     KDKVLQDVRAKVDLARGRFPPDAEEAIIQENNVNDDPVLGIMLSGAAPERALYQTARE
                     LQDRIESLEGVLRTDLGGGREELLEVTIDPVRMEAANVTTGELAQVIGRNNQLVPAGN
                     LQTQGGKFAVKVPGVIERPEDILALPIKKNGDRLITVGDIGEVRRTFKEPTRITRFNG
                     QPAFAIDVVKRSGANVLDTVAAVRKVVETEQKRWPETIKVDFTYDESEFINRGLVVLE
                     SGLITATLLVMVIIIASLGLRQGLMVGLAIPLCFMIAFLLLQAFGITLNQMVMFGLIL
                     AVGILVDGGIVVVEYADRKQAEGMPKQEAFAAAGKRMFWPVVNGTLTTLCAFIPFMFW
                     NSIAGKFMSFLPITLFMVLGASIFVALIFTPALGSIAGRKTGIDEEHLEEIHKSEHGD
                     PRRMAGFMGWYARVLWSSGHHPFRVTAIAMVVIVAIFMWFVKTPHRSEFFLDQDPEWA
                     TVYVKARGNLSVEAANALVQQVEARLVGVKGMESIYASAGQFNGFGFGGPPNDTIGRL
                     RIQFVDFEARHELGIRGQQVVDDIRKRVANIPGILTEVQLPQGGPPQGKDIQVELRSS
                     DPAALNRAADLIKAHLADDPQLIEQEDTRASPGIEWNLTVDRIAAGRYGVDVLQVGQA
                     IQFVTNGILVGRFRPDDAEDELDIRVRFPPDARNMAAFDRLKITTPQGPVPASYFVTR
                     TPAQQVTSIQRRDAQRLVIIKANTIDGVAANQKIAALRPWLEKAPIDPSVRWMFRGAD
                     EEGQKAAVFFMVAMLATLFMMSVILLWQFNSFYGVVVTLSAVILSTIGVFLGVQVNLL
                     HTFDYISVIMLGTGIIALAGVVVGHNIVLVDTYYQLRRQGFEADVAAMRAAVQRFRPV
                     MLTTLVTIVGLLPLMFQIHPNFRVGDIEYKSPGSEWWVQLSGAVVWGLSFATLLTLFL
                     TPAALAAPKTLARRFRNVWLALRRKQAWADRQRTDEEGRVADDGYQDYRKAAE"
     gene            complement(5254..>5855)
                     /locus_tag="PLO65_08075"
     CDS             complement(5254..>5855)
                     /locus_tag="PLO65_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD28241.1"
                     /translation="TLVRLALRLIDPQSGRVTIDGVDLKALGMASLRRAVALVPQDVA
                     LFNDTLEANIAFGRPDASSAEILAAAEAAELSGFIAGLPDGMATRVGERGLKLSGGER
                     QRVGLARALLADPRVLILDEATSALDSRTEAAIQETLRRARQGRTTLVVAHRLSTIAD
                     ADEILVLKKGRIVERGAHEALLAAGGEYATLWKRQTRGR"
BASE COUNT          890 a         2012 c         1935 g          857 t
ORIGIN      
        1 acgaagagga aggccggggc cccggggccc ccgttgagat atttgtagcc gcagccgatc
       61 gcgaggtcgg cgttgcagcc gttcaggtcg acctcgagca cgcccgtgct gtggctcagg
      121 tcccacagca ccagggcgcc ggcggcctgc gccctcgccg tcagggcggc catgtcgcgc
      181 accgcgccgc tgcgatagtg ggtctggcag agcaccagca gcgcgnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnngcga cggccagctt gtagagattg gccgaggtcg
      421 aatcggcgac cagcacctcg tccggatggg ccccgatcag cccggcgatc ttgccgccca
      481 cccgggcggg ggcgccgaac cagtcgtggc tgttccagcc gcgcacgagg ctttcgcccc
      541 attcacgctc gaccatgcgg gcgacgcgac tggccgcctc gcgcggcggc gggcccagcg
      601 aattgccggc cagatagacc agcccgcccg gccgcgcgaa ggcgtcgcga aagccggcca
      661 gccggtctgc ggcgtccagc gccacggggt cgagggagtc catgcgtgac ttatccgccg
      721 cacacgcccc gctgaccagc ggacgaaacg gaacgtttca ggaatttcat ttgcgggtca
      781 cccacatttt gaggataccg aacactgttc agattgcgcc cgggggctgc tacgaggcgc
      841 gcgtcgcgcg cccggcgccc gggttgcgcc cgggattcga gatgcagatc aaacgctcct
      901 acctcatcgc cggcctcatc gtggccgcct tcctgctgtt cttcatcggc gggacggtgc
      961 tgcgcgccct ggcccccaag gaccgcccgg cggaaaccgc ggcggccaag gcttcggcgg
     1021 ccaaggccga cgagccgctg gtcaagatcg ccgtgatcaa cgaggtccag cgaccctacg
     1081 tggtgtccgt ccgcggccgc accgacgcca accggaccgt gatcgtgcgg gcggagaccg
     1141 ccggcccggt ggccgcgacc ccggcccgcg aaggatcctt cgtcgccaag ggcgcgatcc
     1201 tctgccagct gtctgtcgat gcgcgccagg ccacgctcga ccaggcccgc gcggccctga
     1261 agtcgcgcca gttgcagcgc gaagcgtccg aacggctcgc cgcgcagggc taccgctcgc
     1321 agacccaggt gctgcaggac caggcgaacc tggacgcggc ctccgcgtcg gtgcgtcagg
     1381 ccgaggtggc gctggaacag gtcaatgtac gggcgccgtt cgccggcgtg ttcgacagcc
     1441 gggacgccga ggtgggctcc tacctcgccc ccggcgcgcc gtgcggcacg atgatccagc
     1501 tcgacccgct ggtcatcgtc gcggacgtat ccgaggccga ggtcggcagc attcgcgtcg
     1561 gggctgcggc gcgggccatg ctggtgtcgg gcgagagcct gaccggccgg gtccggtcgg
     1621 tcggccgcaa cgccgacccg gcgacccgca cctaccgcgt cgagatcgag gcgcggaacc
     1681 cgggcctgcg cgtgcggtcc ggcctgtccg ccgacgtcaa gatcaccgtc ggcgccgggc
     1741 cggccatgct ggttccggcc gccaccctgg tgctcgattc ggccggccgg cagggcgtcc
     1801 gcttcgtcgg ccaggacgag cgggtcggct tcgccaacgt ccaggtccag gacgaaacgg
     1861 ccgagggcat ctggatcacc ggactgcgcg gccccgcccg ggtcatcgtc gccggtcagt
     1921 cctacgtgtc tgaaggccag aaggttcgcg cgtcggcgcg ctgagcagcg cccggtcgcc
     1981 aggagataga gtgagatgat cggagccctg atcgacggcg caatcaagcg gcgcaaggtc
     2041 gtgctcggca tcgccgcgat cgcgtccctg ttcggcctgt tcgcctacat gaacctgccg
     2101 cgggaatcgt caccgaacat cccggtgccg ttcatcgcca tcacggtgcc ctaccccggg
     2161 gtaagtcccg aggactcaga gcggctgctg gtgcggccgt tggagcgcga actgcagtcc
     2221 ctcgagggcc tgaaggagat gaacgccgtc gcgcagcaga acgccgcgac cgtggtgctg
     2281 gagttcgagg tcaacttcga caaggacaag gtgctgcagg acgtccgcgc caaggtcgac
     2341 ctggcccgcg gccgtttccc gcccgacgcc gaagaagcga tcatccagga aaacaacgtc
     2401 aacgacgacc cggtgctggg catcatgctg tcgggcgccg cgcccgagcg cgccctctac
     2461 cagaccgccc gcgaactgca ggaccgtatc gagagcctcg agggcgtgct gcgcaccgac
     2521 ctcggcggcg gtcgcgagga actgctcgaa gtcaccatcg atcccgtccg gatggaggcg
     2581 gccaacgtca cgaccggcga gctggcgcag gtcatcggcc gcaacaacca gctggtgccc
     2641 gccggcaacc tgcagaccca gggcggcaag ttcgcggtga aggtgcccgg cgtcatcgag
     2701 aggcccgagg acatcctcgc cctgccgatc aagaagaacg gcgaccggct gatcacggtc
     2761 ggcgacatcg gcgaggtgcg gcggaccttc aaggaaccga cgcggatcac ccgcttcaac
     2821 ggccagccgg ccttcgccat cgacgtggtc aagcgctcgg gcgccaacgt gctcgacacc
     2881 gtggccgccg tgcgcaaggt agtcgagacc gagcagaagc gctggcccga gacgatcaag
     2941 gtcgacttca cctacgacga aagcgagttc atcaaccgcg gcctggtggt gctggagtcc
     3001 ggcctgatca ccgccaccct gctggtcatg gtgatcatca tcgccagcct gggcctgcgc
     3061 cagggcctga tggtcgggct ggcgatcccg ctgtgtttca tgatcgcctt cctgctgctg
     3121 caggccttcg gcatcaccct caaccagatg gtcatgttcg gcctgatcct ggccgtcggc
     3181 atcctggtcg acgggggcat cgtcgtcgtg gagtacgccg accggaagca ggccgagggc
     3241 atgcccaagc aggaggcctt cgcggccgcc ggcaagcgca tgttctggcc cgtggtcaac
     3301 ggcacgctga cgaccctgtg cgccttcatc ccgttcatgt tctggaactc gatcgccggg
     3361 aagttcatga gcttcctgcc gatcaccctg ttcatggtgc tcggcgcgtc gatcttcgtg
     3421 gccctgatct tcaccccggc cctgggctcg atcgccggcc gcaagacggg gatcgacgag
     3481 gagcacctgg aggagatcca caagtccgag cacggagatc cgcgcaggat ggccggcttc
     3541 atgggctggt acgcccgggt cctgtggtcg tcgggccacc atccgttccg cgtgaccgcc
     3601 atcgccatgg tggtgatcgt cgcgatcttc atgtggttcg tgaaaacgcc gcaccgcagc
     3661 gagttcttcc tcgaccagga cccggagtgg gccacggtct atgtcaaggc gcgcggtaac
     3721 ctgtccgtcg aggcggccaa cgcgctggtg cagcaggtcg aggcgcgcct ggtcggggtc
     3781 aagggcatgg agtcgatcta cgcctccgcc ggccagttca acggcttcgg cttcggcgga
     3841 ccgcccaacg acacgatcgg tcgcctgcgc atccagttcg tcgacttcga ggcgcggcac
     3901 gagctgggca tccgcggcca gcaggtcgtc gacgacatcc gcaagcgggt ggccaacatc
     3961 cccggcatcc tgaccgaggt gcaactgccc cagggcggac cgccgcaggg caaggacatc
     4021 caggtcgagc tgcgcagctc agatccggcg gcgctcaatc gcgcggcgga cctgatcaag
     4081 gcgcacctgg ccgacgatcc ccagctgatc gagcaggagg acacccgcgc ctcgcccggc
     4141 atcgaatgga acctgaccgt cgaccgcatc gccgccggcc gctacggcgt ggacgtgctg
     4201 caggtcggcc aggcgatcca gttcgtcacc aacggcattc tcgtcggccg cttccggccc
     4261 gacgacgccg aggacgagct ggacatccgc gtccgcttcc cgcccgacgc gcgcaacatg
     4321 gccgcattcg accggctcaa gatcaccacg ccgcagggtc cggtgccggc gagctatttc
     4381 gtcacccgca cacccgccca gcaggtgacc tcgatccagc gtcgcgacgc ccagcgcctg
     4441 gtgatcatca aggccaacac catcgacggc gtggccgcca accagaagat cgccgcgctc
     4501 aggccctggc tcgagaaggc gccgatcgac ccgtccgtcc gctggatgtt ccgcggcgcc
     4561 gacgaggaag gtcagaaggc ggctgtcttc ttcatggtcg ccatgctggc cacgctgttc
     4621 atgatgtcgg tcatcctgct gtggcagttc aacagcttct acggcgtggt ggtgacgctg
     4681 tcggccgtga tcctgtcgac catcggcgtg tttctgggcg tgcaggtcaa tctgctgcac
     4741 accttcgact acatctcggt gatcatgctg gggaccggca tcatcgccct ggccggggtg
     4801 gtcgtgggcc acaacatcgt gctggtcgac acctactacc agctgcggcg gcaaggcttc
     4861 gaggccgacg tggcggccat gcgggcggcc gtacagcggt tccggccggt catgctgacc
     4921 accctggtga ccatcgtcgg cctgctgccg ctgatgttcc agatccaccc gaacttccgc
     4981 gtcggcgata tcgagtacaa gtcgccgggt tcggaatggt gggtccagct gtcgggcgcc
     5041 gtcgtgtggg gcctgagctt cgccaccctg ctgaccctgt tcctcacgcc ggcagccctg
     5101 gccgcgccca agaccctggc ccggcgattc cgcaatgtct ggctggccct tcgccgcaag
     5161 caggcctggg ccgaccggca gcgcacggac gaggagggcc gcgtggccga cgacggctac
     5221 caggactacc gcaaggccgc cgagtagcgt ggtctaacgg ccgcgggtct ggcgcttcca
     5281 cagggtggcg tattcgccgc ccgccgccag cagcgcctcg tgcgcgccgc gctcgacgat
     5341 cctgcccttc ttgaggacca ggatctcgtc cgcgtcggcg atcgtcgaca ggcggtgggc
     5401 cacgaccagc gtcgtgcggc cctggcgggc gcgccgcagc gtctcctgga tggccgcctc
     5461 ggtgcgcgag tcgagcgcgc tggtcgcctc gtcgagaatc agcacgcgcg gatcagccag
     5521 cagcgcgcgg gccaggccga cgcgctggcg ctcgccgccc gacaacttga gcccccgctc
     5581 gccgaccctc gtggccatgc catcgggcag gccggcgatg aagcctgaca gctccgccgc
     5641 ctcggccgcc gcgaggatct ccgcggacga ggcgtccgga cggccgaacg cgatgttggc
     5701 ctccagggtg tcattgaaca gggccacgtc ctggggcacc agcgccacgg cccggcgcag
     5761 ggacgccatc ccgagggcct tcaggtcgac gccatcgatg gtcacccgcc cggactgcgg
     5821 atcgatcagc cgcagggcca gccgcaccag ggtgg
//
LOCUS       DAORNX010000152         5845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10126_length_5845_cov_7.270294, whole genome shotgun sequence.
ACCESSION   DAORNX010000152 DAORNX010000000
VERSION     DAORNX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10126_length_5845_cov_7.270294"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="PLO65_08080"
     CDS             <1..1903
                     /locus_tag="PLO65_08080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD28242.1"
                     /translation="VAPAPVVAGVPPLPPAELEAYVDGLVRDAMAADHIPGVTVSVVQ
                     NGQVVLMKGYGFANLGPARPVDPDRTLFRIASISKTFTWIAVMKEVEAGRMRLDAPIN
                     LYLPEKIRVPDQGFDRPVRIIDLMSHSPGFEDRALGQLFERDPDRVRPLQLYLRQERP
                     RRVREPGATPSYSNYGVALAGAAASWVSGKPFERLIESEITGPLGMARTTFRDPYPPR
                     KDLPAPMPAALAADLADGFLWTDAGWTRRPYEFTSQVAPAGGASTTAADMARYMMMML
                     NGGSLDGAQIYGPATAAGFARVQSRPAPGVAGFAHGLIEVQLPGGRVGWGHDGALLSF
                     RSNMMVAPDLGLGVFVATNSETGGRLTAQLAGRIVQRFYGPAPTLPVAGSPWLKQNAR
                     AFQGEFVSTRRSYRGLEQFIGLIDSRVRIAVTDDGYLRIRGRDSSSLWAPEGTGGRFR
                     EVGGWRRMAFVMTNGRATGYYPPSGAVMFQRVGFFGGQAWLVNLAMLAAIAALATLAG
                     LLMRDRKDHRETPMQRRASLIQTTQAILWLLAMGALAIWAGKAGDVAAIFFDWPGGWM
                     LTASSCAFVAAILSGVTLFLLPFIWRGGRRVESWTVGRKLRFTVTTLVYVGLSFVLAF
                     RGALVPWAS"
     gene            1989..2408
                     /locus_tag="PLO65_08085"
     CDS             1989..2408
                     /locus_tag="PLO65_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5367 family protein"
                     /protein_id="HRD28243.1"
                     /translation="MSKEDGLTFALAGLVAFLVSTAWYAAFGTGLLEKAFWFYAANAF
                     LVGGAVVFLFHVAARLRRLPRRRRPLAAAVFAAPGLLGAAIMLMLFKDIFPQWDPVSL
                     GRYGAFILLGDALLAGVALDRSRARAAGATTAASRAR"
     gene            complement(2383..2736)
                     /locus_tag="PLO65_08090"
     CDS             complement(2383..2736)
                     /locus_tag="PLO65_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HRD28244.1"
                     /translation="MTDDWTSHGKVRARESGGTLELVVDGLTTQAKYYKPLIYEFFRK
                     QWRGGRPAWGDFAVEIIMEYEGDPPWLDLDNLAKAILDSIKGHAFHDDSQVARLLVER
                     RQGARERITVRATRL"
     gene            2817..4208
                     /gene="fumC"
                     /locus_tag="PLO65_08095"
     CDS             2817..4208
                     /gene="fumC"
                     /locus_tag="PLO65_08095"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRD28245.1"
                     /translation="MTATRTETDTFGPIEVAADRYWGAQSQRSLGNFRIGWEKQPLPV
                     VRALGIVKRAAAETNLALGKLDPKIAETIVKAANEVIEGKLNDHFPLVVWQTGSGTQS
                     NMNANEVISNRAIEMLGGEMGSKKPVHPNDHVNMSQSSNDTYPTAMHIACAEQVVHDL
                     LPALKHLHAALKAKSEAWQGIIKIGRTHTQDATPLTLGQEFGGYAQQVANGIHRIEQT
                     LPQLMELAQGGTAVGTGLNAPVGFAEKVAERIAAITGLPFTTAPNKFEALAAHDAMVF
                     SHGAINTVAASLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQCEALT
                     MVCTQVFGNHAAITFAGASGHFELNVFNPVMAYNFLQSVRLVADAAISFTDNCVVGIE
                     PREDNIKRGLDNSLMLVTALNGKLGYDACAKIAKTAHKNGTTLREEAVGGGYLTDAEF
                     DEAVRPEKMISPG"
     gene            4250..5005
                     /locus_tag="PLO65_08100"
     CDS             4250..5005
                     /locus_tag="PLO65_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD28246.1"
                     /translation="MTGLVRPYWIAAEFRRDEGWDDGVYPFNLPVVNRTDRIDFHPRV
                     TFLVGENGSGKSTLIEALAVAWGFNPEGGSKDHRFATHDSHSSLHRHIRAIRDAMQPK
                     PHDGFFLRAESFFNVATYLEQAGGSRYGDRPLHQQSHGEAFFSLFENRLTGDGLYIMD
                     EPEAALSPQRQLSFLAKLHQLVLARSQLIIATHSPIILGYPDAWIYQASEHGLERIDY
                     EDTEHYSVTRGFLNRRKTFLKILMEEDEGPAPR"
     gene            complement(5041..5439)
                     /locus_tag="PLO65_08105"
     CDS             complement(5041..5439)
                     /locus_tag="PLO65_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRD28247.1"
                     /translation="MRLLLNILWFIFGGFVSGLLWLFGGLVLAVTIVGLPYTMAAWRI
                     AGFAFWPFGKEIVSREIVTGEHDLGTGPLGFLLNVIWFLFAGWYIALSHIVAAVLEAV
                     TIIGLPFAFKDLQLAGIAMAPVGKIVVEKR"
     gene            5464..>5845
                     /locus_tag="PLO65_08110"
     CDS             5464..>5845
                     /locus_tag="PLO65_08110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28248.1"
                     /translation="MSDVVIILRLFEGLPHGFDVMCAEAGGEGIRNMAMLRSEVGAPD
                     DPFVDPGALFAATVGGQLAGVGGVTVQTGLAEPAMRMRRLYVRPTFRRAGVARALAGA
                     MRGSAPQGEALAGKRLRANSTSAAS"
BASE COUNT          910 a         2064 c         1954 g          917 t
ORIGIN      
        1 cgtcgcgccc gcgccggtcg tcgccggcgt gccccccctg ccaccggcgg agctggaggc
       61 ctacgtcgac ggcctcgtgc gcgacgccat ggccgccgac cacattccgg gcgtcaccgt
      121 ctcggtggtc cagaacggcc aggtcgtgct gatgaagggc tacgggttcg ccaacctcgg
      181 tcccgcccgg ccggtcgatc ccgaccgcac cctgttccgc atcgcctcca tctccaagac
      241 cttcacctgg atcgcggtca tgaaggaggt cgaggccggc cgcatgcggc tcgacgcgcc
      301 gatcaacctc tacctgcccg agaagatccg ggttcccgac cagggtttcg accggcccgt
      361 ccggatcatc gacctgatga gccattcgcc aggcttcgag gaccgcgccc tgggccagtt
      421 gttcgagcgg gaccccgatc gcgtgcggcc gctgcagctc tacctgcgcc aggaacgccc
      481 gcgacgggtg cgcgagcccg gcgcgacgcc gtcctattcc aactacggcg tggccctggc
      541 gggggcggcg gcgtcctggg tatcgggcaa gccgttcgag cgcctgatcg agagcgagat
      601 cacaggcccc ctgggcatgg cccgcacgac gttccgggac ccctacccgc cccgcaagga
      661 cctgccggcg ccgatgccgg cagctctggc ggccgacctc gccgacggct ttctgtggac
      721 cgacgccggc tggacccgcc ggccctacga attcacctcc caggtcgccc ccgccggcgg
      781 cgcctcgacc acggcggccg acatggcccg ctacatgatg atgatgctca acggcggcag
      841 cctggacggc gcgcagatct acggtccggc gacggcggcc ggcttcgccc gcgtgcagtc
      901 gcggccggcg cccggggtgg ccggattcgc ccatggcctg atcgaggtcc agctgccggg
      961 cggtcgcgtt ggttggggtc atgacggcgc cctgctgtcg ttccgctcga acatgatggt
     1021 ggcgccggac ctgggccttg gcgtgttcgt ggcgaccaac tccgagacgg gcggccgcct
     1081 gaccgcacag ctcgccgggc ggatcgtcca gcggttctac ggtcccgccc ccaccctgcc
     1141 ggtcgccggc tcgccctggc tgaagcagaa cgcccgcgcc ttccagggcg agttcgtcag
     1201 cacgcgccgt tcctatcgcg gcctggaaca gttcatcgga ctgatcgaca gccgcgtccg
     1261 gatcgcggtg actgacgacg gctacctgcg aatccgcggc cgtgactcct cgtcgctctg
     1321 ggcgcccgag ggaaccggcg gccgtttccg cgaggtcggc ggctggcgtc ggatggcgtt
     1381 cgtcatgacc aacggccggg ccaccggcta ctatccgccg tccggcgcgg tgatgttcca
     1441 gcgcgtcggc ttcttcggcg gccaggcgtg gctggtcaat ctggccatgc tggccgcgat
     1501 cgccgccctg gcgaccctgg ccgggctgct gatgcgcgac cgcaaggatc accgcgagac
     1561 gccgatgcag cgtcgggcca gcctcatcca gacgacccag gcgatcctct ggctgctggc
     1621 catgggcgcc ctggcgatct gggccggaaa ggccggggac gtggcggcga tctttttcga
     1681 ctggcccggg ggctggatgc tgacagcctc gtcctgcgcc ttcgtggcgg ccatcctgtc
     1741 gggagtgacc ctgtttctgc tgccgttcat ctggcgcggc gggcggcggg tcgaaagctg
     1801 gaccgtgggc cgcaagctgc gtttcacggt caccaccctg gtctatgtcg gcctgtcgtt
     1861 cgtgctcgcc ttccgcggcg cgctcgtgcc ttgggcgagc tgacttttca gacccgcgac
     1921 catcggccac ctttctggcg ccgccctcgc atccgcagga tcgaaacggg tggttgaggg
     1981 tggttgtcgt gtcaaaagaa gacggtctga ccttcgctct ggcgggactt gtggcctttc
     2041 tggtctcgac cgcctggtac gcggctttcg ggacgggact gctggagaag gcgttctggt
     2101 tctacgccgc gaacgccttc cttgtcggcg gggccgtggt gttcctgttc catgtcgccg
     2161 cgcgactgcg ccgcctgccg cgtcgcaggc gtcctctggc ggcggccgtg ttcgccgccc
     2221 ccggcctgct cggggcggcg atcatgctga tgctgttcaa ggacatcttc ccgcaatggg
     2281 acccggtcag ccttggccgc tacggcgcct tcatcctgct cggcgacgcc ctgctggcgg
     2341 gggtggcgct ggaccggtca cgagcgcgcg ccgccggagc gactacagcc gcgtcgcgcg
     2401 cacggtgatc cgttcgcggg cgccctgacg gcgctcgacc agcaacctgg cgacctggct
     2461 gtcgtcatgg aaggcatggc ccttgatcga gtcgaggatc gccttggcca ggttgtcgag
     2521 atcgagccag ggcggatcgc cctcgtactc catgatgatt tcgacggcga agtcgcccca
     2581 ggccggccgc ccgcctcgcc actgcttgcg gaagaactcg tagatcagcg gcttgtagta
     2641 tttcgcctgg gtcgtcaggc cgtcgacgac cagctccagc gtgccaccgc tctcgcgggc
     2701 gcggaccttg ccatggcttg tccagtcgtc ggtcattggg gtccataacg ccttgcgcgc
     2761 ttgcagtctc gccctccggc gctaaaccgg cgctgcaagt ttcgccggga aagatcatga
     2821 ccgccacgcg caccgagacc gacaccttcg gccccatcga ggtcgccgcc gaccgctact
     2881 ggggcgccca gtcgcagcgc agcctgggca acttcaggat tggctgggaa aaacagcccc
     2941 tgcccgtcgt gcgggccctg ggcatcgtca agcgcgcggc cgccgagacc aatctggcgc
     3001 tgggcaagct ggacccgaaa atcgccgaga ccatcgtcaa ggcggccaac gaggtcatcg
     3061 agggcaagct gaacgatcac ttcccgctgg tggtctggca gaccggctcg ggcacccagt
     3121 cgaacatgaa cgccaacgag gtgatctcca accgcgccat cgagatgctg ggcggcgaga
     3181 tgggctccaa gaagccggtc cacccgaacg accacgtcaa catgagccag tcgtcgaacg
     3241 acacctatcc gacggccatg cacatcgcct gcgccgagca ggtggtccac gatctgctgc
     3301 cggccctcaa gcacctgcac gcggcgctga aggccaagag cgaggcctgg cagggcatca
     3361 tcaagatcgg ccgcacccac acccaggacg cgacgcccct gaccctgggc caggagttcg
     3421 gcggctacgc ccagcaggtc gccaatggca tccaccgcat cgagcagacc ctgccgcagc
     3481 tgatggagct ggcccagggc ggcacggccg tcggcacggg cctgaacgcg ccggtgggct
     3541 tcgccgagaa ggtcgccgag cgcatcgccg cgatcaccgg cctgccgttc accaccgccc
     3601 cgaacaagtt cgaggccctg gccgcccacg acgccatggt gttcagccac ggcgccatca
     3661 acacggtcgc cgccagcctg ttcaagatcg ccaacgacat ccgcttcctc ggctcgggcc
     3721 cgcgctcggg cctgggcgaa ctgagcctgc cggagaacga gccgggcagc tcgatcatgc
     3781 cgggcaaggt caatccgacc cagtgcgagg ccctgaccat ggtctgcacc caggtgttcg
     3841 gcaatcacgc cgccatcacc ttcgccggcg ccagcggcca cttcgagctg aacgtgttca
     3901 acccggtgat ggcctacaac ttcctgcagt cggtgcgcct ggtggccgac gcggcgatca
     3961 gcttcaccga caactgcgtc gtcggcatcg agccgcgcga ggacaacatc aagcgcggcc
     4021 tcgacaacag cctgatgctg gtcaccgccc tgaacggcaa gctcggctac gacgcatgcg
     4081 ccaagatcgc caagaccgcc cacaagaacg gcaccaccct gcgcgaggaa gccgtcggcg
     4141 gcggctacct gacggacgcg gagttcgacg aggccgtgcg gccggagaag atgatctcgc
     4201 cggggtgagc ccacttccgg ttgttccggg attgttctag aagaaggcca tgaccggcct
     4261 tgtccgcccc tactggatcg ccgccgagtt tcggcgcgac gagggctggg acgacggcgt
     4321 ctatcccttc aacctgcccg tggtgaacag gaccgaccgc atcgacttcc acccgcgcgt
     4381 gaccttcctg gtcggcgaga acggctcggg caagtcgacc ctgatcgagg cgctggccgt
     4441 cgcctggggc ttcaatccgg agggcgggtc gaaggaccac cgcttcgcca cgcacgacag
     4501 ccactcgagc ctgcaccggc acatccgggc gatccgcgac gcgatgcagc caaagcccca
     4561 cgacggcttc ttcctgcgcg cggagagctt cttcaacgtc gccacctatc tcgaacaggc
     4621 gggcggcagc cgctacggcg accggccgtt gcaccagcag tcgcacggcg aggccttctt
     4681 cagcctgttc gagaaccggc tgacgggcga cggcctgtac atcatggacg agccggaagc
     4741 ggcgctgtcg ccgcagcgcc agctgtcatt cctggccaag ctgcaccagc tggtgctggc
     4801 ccgctcgcag ctgatcatcg ccacccactc gccgatcatc ctcggctacc ccgacgcctg
     4861 gatctaccag gcctctgagc acgggcttga gcggatcgac tacgaggaca ccgagcacta
     4921 cagcgtcacc cggggcttcc tcaatcgtcg caagaccttc ctgaagatcc tgatggaaga
     4981 ggacgagggg ccggcgccgc gctgaccggg gacatgcact tcagtgcgtg tcgccgcctg
     5041 ctaccgcttc tccaccacga tcttgccgac cggggccatg gcgatgccgg ccagctgcag
     5101 gtccttgaac gcgaacggca ggccgatgat ggtgaccgcc tccaggacgg ccgccacgat
     5161 gtgcgacagg gcaatgtacc agccggcgaa caggaaccag atcacgttga gcaggaagcc
     5221 cagcggcccg gtgccaaggt cgtgctcgcc ggtgacgatc tcgcgcgaga cgatctcctt
     5281 gccgaacggc cagaaggcga agccggcgat gcgccaggcg gccatcgtgt acggcaggcc
     5341 gacaatcgtc acggccagca ccagaccgcc gaacagccac agcaggccgc tgacgaaccc
     5401 gccgaagatg aaccagagga tgttgagcag caggcgcatg gacggggttc cggaaacgac
     5461 gtgatgtctg acgtggtgat catcctaagg cttttcgagg ggctgccgca tggattcgat
     5521 gtcatgtgcg ccgaggcggg cggcgaggga attcgcaaca tggccatgtt gcgaagcgag
     5581 gtcggcgcgc cggacgatcc gttcgtcgac ccgggcgcgc tgttcgcggc taccgtgggg
     5641 gggcagctgg cgggcgtggg cggcgtcacg gtgcagaccg gactggccga acccgccatg
     5701 cgcatgcgtc gcctctacgt gcggcccacc ttccgccgcg cgggggtggc gcgggcgctg
     5761 gccggggcga tgcgcggcag tgcaccccag ggcgaggcgt tggccgggaa gcgcttgcgc
     5821 gcgaactcga cctccgcggc ttcgg
//
LOCUS       DAORNX010000153         5840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10136_length_5840_cov_7.001210, whole genome shotgun sequence.
ACCESSION   DAORNX010000153 DAORNX010000000
VERSION     DAORNX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_5840_cov_7.001210"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLO65_08115"
     CDS             <1..409
                     /locus_tag="PLO65_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRD28249.1"
                     /translation="SGILVESGQHAGGGLWVAVGIGVNLATPPEAPERPATALAEHMA
                     GPPPAPLEALAVLAESFERWLRLWRASGFAPVAEAWTRRAHGLGEVCTARLGHETVTG
                     TAEGLDADGALRLRLPDGMLRRITAGDVFFGNI"
     gene            410..1192
                     /locus_tag="PLO65_08120"
     CDS             410..1192
                     /locus_tag="PLO65_08120"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRD28250.1"
                     /translation="MLLAIEQGNTNTLFAVHDGETWIAQWRSATESTRTADEYAVWLS
                     QLFEMNGLSLKSLNGCIISSVVPQSIFNLRNLSRRYLHVEPLIIGENAELGVAVRIDK
                     PSEAGADRLVNAIGAHIAYPGPLVVIDSGTATTFDIVAADGGFEGGIIAPGVNLSMQA
                     LHDAAAKLPRIAIQRPEGNRVVGTDTVSAMQSGVFWGYIGLIEGLIQRIKAERGEHLT
                     VVATGGVVSLFEGATDMIDHFDPDLTIRGLLEIYRRNASPRA"
     gene            1192..2871
                     /locus_tag="PLO65_08125"
     CDS             1192..2871
                     /locus_tag="PLO65_08125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRD28251.1"
                     /translation="MKPRKNDELVFLPLGGSNEIGMNFNLYGYGPEDDRKWIVVDLGI
                     TFGDQTTPGVEVILPDPTFIEDYADDILGIVLTHAHEDHIGAVAWLWPRLKAPVYATP
                     FTAFLLREKLRDAGLLDEVDITEVPLSGRFALGPFELELITLTHSIPEPNGLAIRTPL
                     GVVLHTGDWKIDPDPMLGEVTDVEAIRRLGDEGVLAMVCDSTNVFVDGSAGSEADVRK
                     ALSDLIKPLTGKVAVACFASNVARMDSVIRAAEAAGRRVCLVGRSMHRMSAAAHSVGL
                     MGGVKDFISEKEARHFPQDKILYLCTGSQGEPRAALARIASGTHPHVNLGKGDHAIFS
                     SRVIPGNEVAIRNLQNHLADRGVRLCTERDHPGIHVSGHPCRDELAQMYAWARPQIAV
                     PTHGERRHLLEHAAFARDLQVPQTVSPRNGDMVRLAPGRAEIIDEVTAGRLYVDGGMV
                     TPENGEALKERRHAAFNGVLMVTIALDGKGRIVSGPQIRAVGLAGDADFPLEEALDDL
                     ALDAEQAVKTIPAEERAFDEIVESALSRVVKKGAFRIWERKPVVETTVLRI"
     gene            2930..3352
                     /gene="mce"
                     /locus_tag="PLO65_08130"
     CDS             2930..3352
                     /gene="mce"
                     /locus_tag="PLO65_08130"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRD28252.1"
                     /translation="MIGKLNHVGVATPSIDESLKMYRDLLGATSHTDKWAMPEQGVWV
                     CFVNLPNSQIELIEPYGEKSPIHGFLAKYPKGGQHHICFEVENIHEARDALVAKGATV
                     LGEPRIGAHGTLIIFVHPKDMGGMLVELMETPKEGHHA"
     gene            3349..3609
                     /locus_tag="PLO65_08135"
     CDS             3349..3609
                     /locus_tag="PLO65_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HRD28253.1"
                     /translation="MTLATGIAIYLTLWWTALFAILPLGNKTYAESGIVVKDGGDPGA
                     PVNPNLKRKFITTTWVSAVLWGVLWLVIRFGGLTLPSLPNNY"
     gene            3898..5226
                     /locus_tag="PLO65_08140"
     CDS             3898..5226
                     /locus_tag="PLO65_08140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRD28254.1"
                     /translation="MRLSRYFLPVLKEAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLP
                     LGLRVLKKIEQIVREEMDRAGAVELLMPTIQLADLWRESGRYEAYGDEMLRIVDRHKR
                     DLLYGPTAEEVVTDIFRAYVKSYKDLPLNLYNIQWKFRDERRPRFGVMRGREFLMKDA
                     YSFDLDEAGARAAYNRMFVAYLNLFARMGLKAVPMKADTGPIGGDLSHEFIVLAETGE
                     SQVFCHKDLVEMGAPGPDVDWFGDLQPLVDRRTALYAATEEMHDAAAFAAVPAADQLS
                     ARGIEVGHIFYFGQKYALPMKASVTGPDSQEHTVHMGSYGVGVSRLLGAIIEASHDEA
                     GIIWPESVAPFDVALINLRPGDEACDAACEQAYAAMTAAGKTALYDDTGERPGGKFAK
                     ADLIGLPWQLIIGPKGLADGVVEIKNRATGERSTAPLAEVLAGLTAKAAA"
     gene            5223..>5459
                     /locus_tag="PLO65_08145"
     CDS             5223..>5459
                     /locus_tag="PLO65_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HRD28255.1"
                     /translation="MSKPAGSVAAPVGLWERAVAGRYLRARRKHGGVALISLISFIGI
                     MLAVMVLIVVMSVMNGFRTELLDRILGFKGHAYVT"
     assembly_gap    5460..5599
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5600..>5840
                     /locus_tag="PLO65_08150"
     CDS             <5600..>5840
                     /locus_tag="PLO65_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28256.1"
                     /translation="LIFSVVMDVAGAAVIAGPHGFEDLCARRTLRMLGDRLAASLGVA
                     PGDSVTLISPAGSSTAFGTSVTQKDYTIGGIFSVG"
BASE COUNT          922 a         1963 c         1921 g          894 t
ORIGIN      
        1 cagcggcatc ctcgtggagt ccggccagca tgcgggcggc ggcctttggg tcgcggtcgg
       61 gatcggcgtc aacctggcga cgccgcccga ggcgccggag cgcccggcca cggcccttgc
      121 cgagcatatg gctgggccgc cccccgcgcc gctggaggcg ctcgccgtcc tggcggagtc
      181 gttcgagcgc tggctgcgtc tgtggcgcgc gtccgggttc gcgcccgtcg ccgaggcctg
      241 gacccggcgg gcccacggcc tgggcgaggt ttgcacggcc cgactgggtc atgagaccgt
      301 gaccggaacg gccgagggcc tcgacgccga cggcgcgctg cgtctgcgct tgccagatgg
      361 catgctgcgg cgcatcacgg ccggcgacgt ctttttcggg aacatctgaa tgctgctggc
      421 tatcgagcag ggcaacacca acaccctgtt cgccgtccat gacggcgaga cctggatcgc
      481 ccagtggcgg tcggcgaccg agagcacccg cacggccgac gaatacgccg tgtggctgtc
      541 gcagctgttc gagatgaacg ggctgagcct gaagagcctc aacggctgca tcatctccag
      601 cgtcgtgccg cagtcgatct tcaatctgcg caacctgtcg cggcgttacc tgcatgtgga
      661 gccgctgatc atcggcgaga acgccgagct gggcgtggcc gtgcgtatcg acaagccgtc
      721 cgaggcgggg gcggaccgtc tggtcaacgc catcggcgcc cacatcgcct atccggggcc
      781 gcttgtcgtc atcgacagcg gcacggccac caccttcgac atcgtcgcgg ccgacggcgg
      841 gttcgaaggc ggaattatcg cgcccggggt caatctctcg atgcaggcgc tgcacgacgc
      901 ggcggccaag ttgccgcgca tcgccatcca gcgacccgag ggcaaccgcg tggtcggcac
      961 cgacacggtc agcgccatgc aatcgggggt gttctggggc tacatcggcc tgatcgaggg
     1021 gctgatccag cggatcaagg cggaacgggg cgagcacctg accgttgtcg cgaccggagg
     1081 cgtcgtctcc ctgttcgaag gcgctaccga catgattgac cacttcgacc cggacctgac
     1141 tatccggggc ctgctggaaa tctaccggcg caacgcttcg ccgcgcgcct gatgaagccg
     1201 cgcaagaacg atgagctcgt cttcctgccg ctgggcgggt cgaacgagat cggcatgaac
     1261 ttcaacctct acggctacgg gccggaggat gatcgcaagt ggatcgtcgt cgacctgggc
     1321 atcaccttcg gcgaccagac cacgccgggc gtggaagtga tcctgccgga cccgactttc
     1381 atcgaggact acgccgacga catcctcggc atcgtgctca cccacgccca cgaggatcac
     1441 atcggcgcgg tcgcctggct gtggccccgg ctcaaggcgc cggtctacgc aacgccgttc
     1501 accgccttcc tgctgcgcga gaagctgcgc gacgcgggtc tcctcgacga ggtcgacatc
     1561 accgaggtcc cgctcagcgg caggttcgcg ctcggtccgt tcgagctcga gctgatcacc
     1621 ctgacccact cgattcccga accgaacggc ctggcgatcc gaacgccgct gggcgtggtg
     1681 ctgcacacgg gggactggaa gatcgacccc gatcccatgc tgggcgaggt taccgacgtt
     1741 gaggccatcc gcaggctcgg cgacgagggc gtgctggcca tggtctgcga cagcaccaat
     1801 gtgttcgtcg atggcagcgc cggttccgag gccgatgtcc gcaaggcgct gtcggacctg
     1861 atcaagcccc tgacgggcaa ggtggcggta gcctgcttcg cgtccaatgt cgcccgcatg
     1921 gacagcgtca tccgcgccgc ggaggctgcc ggccgacggg tctgcctggt cggccgctcg
     1981 atgcaccgga tgtccgccgc cgcccattcg gtcggcctga tgggcggggt caaggacttc
     2041 atcagcgaga aggaagccag gcacttcccg caggacaaga tcctctacct ctgcacgggc
     2101 agccagggcg agccgcgcgc ggccctggcc cggatcgcct cgggcacgca tccgcacgtc
     2161 aacctcggca agggcgacca cgcgatcttc tcctcgcggg tcattccggg caacgaggtg
     2221 gctatccgca acctgcagaa ccatctggcc gaccgcggcg tgcggctgtg caccgagcgg
     2281 gaccatccgg gcatccacgt ctcgggccac ccctgccgcg acgaactggc ccagatgtat
     2341 gcctgggccc ggccacagat cgccgtgccg acccatggcg agcgccgcca cctgctggag
     2401 cacgccgcct tcgcccgcga cctgcaggtc ccccagacgg tcagcccgcg caacggcgac
     2461 atggtgcggc tggcccccgg ccgggccgag atcatcgacg aggtcaccgc cggccggctg
     2521 tacgtcgacg gtggaatggt cacgccggag aacggcgagg ccctcaagga acgccgtcac
     2581 gccgccttca acggcgtgct gatggtcacc atcgcgctcg acggcaaggg gcggatcgtc
     2641 tccggtccgc agatccgcgc cgtcggcctg gccggcgacg ccgacttccc gctggaggag
     2701 gcgctcgacg acctggccct cgacgccgaa caggcggtca agacgatccc cgccgaggaa
     2761 cgcgccttcg acgagatcgt cgagagcgcc ctgtcccgcg tggtcaagaa gggcgccttc
     2821 cgcatctggg aacgcaagcc cgtggtggag acgacggtgc tgcggatctg acgtccttca
     2881 ccctccggga gaagggtgtt aggacgccgc tgctccagga gaccagttca tgatcggcaa
     2941 gctcaaccac gtcggggtcg ccacgccctc catcgacgag tcgctgaaga tgtatcgcga
     3001 cctgctgggc gcgacctcgc acaccgacaa atgggccatg ccggagcagg gcgtgtgggt
     3061 ctgcttcgtc aacctgccca acagccagat cgaactgatc gagccctatg gcgagaagag
     3121 cccgatccac ggcttcctgg ccaagtaccc gaaaggcggc cagcaccaca tctgcttcga
     3181 ggtcgagaac atccacgagg cgcgcgatgc gctggtggcc aagggcgcga ccgtgctcgg
     3241 cgaaccgcgc atcggcgccc acggcaccct gatcatcttc gtgcatccca aggacatggg
     3301 cggcatgctg gtcgaactga tggaaacccc caaggaaggt caccacgcat gaccctggcc
     3361 accggaatcg ccatctatct gaccctctgg tggacggcgc tgttcgccat cctgccgctg
     3421 ggcaacaaga cctacgcgga atccggcatc gtggtgaagg acggcgggga tcccggcgcg
     3481 ccggtcaatc ccaatctcaa gcgcaagttc attaccacca cctgggtgtc ggcggtgctg
     3541 tggggcgtgc tgtggctggt gatccggttc ggcggcctga ccctgccgag cctgccgaac
     3601 aactactgaa ttccgcccag gctttcggcg ccgggcctcg cgccgacgcg cggacgccta
     3661 aagtttgggc gatcaacggt cgccgggccg gacgcgagag ccgcgcgctt cgccgccgga
     3721 cgggcggcgc taggctggct tcatggaaga ccggtttccc ggtcttgctc cgtggcgttt
     3781 ttccccgacg atgacctgca aggccgccgg ttcccccggc ggccttcttt tcgccgcccg
     3841 cgttgccaat gcagggccgc agaggctaag tcagccccgg atattcaagg ggttcccatg
     3901 cgcctgtcgc gctatttcct gcctgtgctc aaagaggcgc cgtcggacgc ccagatcgtc
     3961 tcccaccagc tgatgctgcg cgccggcatg atccgccagg aggcggccgg catctacgcc
     4021 tggctgccgc tggggctgcg ggtgctgaag aagatcgagc agatcgtgcg cgaggagatg
     4081 gaccgcgccg gcgccgtcga gctgctgatg ccgaccatcc agctggccga cctttggcgc
     4141 gagagcggcc gctacgaggc ctatggcgac gaaatgctgc gcatcgtcga ccgccacaag
     4201 cgcgacctgc tctacggccc gaccgccgag gaggtggtga ccgacatctt ccgcgcctat
     4261 gtgaagagct acaaggacct gccgctgaac ctctacaaca tccagtggaa gttccgcgac
     4321 gagcgccggc cgcgcttcgg cgtgatgcgc ggacgcgagt tcctgatgaa ggacgcctac
     4381 agcttcgacc tcgatgaggc gggggcccgg gcggcctaca accgcatgtt cgtcgcctat
     4441 ctgaacctgt tcgcgcgcat gggcctcaag gccgtgccga tgaaggccga caccggtccg
     4501 atcggcggcg acctgagcca tgagttcatc gtcctggccg agaccggcga gagccaggtg
     4561 ttctgtcaca aggacctggt ggagatgggc gcgccgggcc cggacgtcga ctggttcggc
     4621 gacctgcagc cgctggtcga ccggcgcacg gcgctctacg ccgccaccga ggagatgcac
     4681 gacgcggcgg ccttcgccgc cgtgcccgcg gcggaccagc tttcggcgcg cgggatcgaa
     4741 gtcggccata ttttctactt tggccagaag tacgccttgc cgatgaaggc cagcgtcacc
     4801 ggcccggaca gccaggagca caccgtgcac atgggcagct acggcgtcgg cgtctcgcgc
     4861 ctgctcggcg cgatcatcga agccagccac gacgaggccg gcatcatctg gccggaaagc
     4921 gtcgcgccgt tcgacgtggc cctgatcaac ctgcggcccg gcgacgaggc gtgcgacgcg
     4981 gcctgcgaac aggcctatgc ggccatgacc gccgccggca agacagccct gtatgacgac
     5041 accggagaac gccctggcgg caagttcgcc aaggccgatc tgatcggcct tccctggcag
     5101 ttgatcatcg gccccaaggg cctggccgac ggcgtggtgg agatcaagaa ccgcgcgacc
     5161 ggcgaacgtt cgaccgcgcc gctggccgag gtgctggccg ggctgacggc gaaggccgcc
     5221 gcgtgagcaa gcccgccggc tccgtcgccg cgcccgtcgg gctgtgggag cgcgccgtcg
     5281 ccggccgcta cctgcgcgcc aggcgcaagc atggcggggt ggcgctgatc tcgctgatct
     5341 ccttcatcgg catcatgctg gccgtgatgg tgctcatcgt cgtgatgagc gtgatgaacg
     5401 gcttccgcac cgagttgctt gaccggatcc tgggcttcaa aggccacgcc tatgtcaccn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnna cctgatcttc tcggtcgtca tggacgtggc gggcgcggcg
     5641 gtgatcgccg ggccgcacgg tttcgaggat ttgtgcgcgc gccgcacctt acgcatgctg
     5701 ggcgaccggc tggcggccag cctcggagtc gcgcctggcg actccgtcac gctgatctcg
     5761 cccgcggggt cgtcgaccgc cttcggcacc agcgtgacgc agaaggacta cacgatcggc
     5821 ggcatcttct cggtcgggat
//
LOCUS       DAORNX010000154         5815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10197_length_5815_cov_6.339583, whole genome shotgun sequence.
ACCESSION   DAORNX010000154 DAORNX010000000
VERSION     DAORNX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_5815_cov_6.339583"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLO65_08155"
     CDS             complement(<1..574)
                     /locus_tag="PLO65_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRD28257.1"
                     /translation="MNTTADTFRRLHSGPDLLILANGWDAGSCRIIETLGAPAVATSS
                     AAVCWSHGYADGHHLPIELLVRTVAEITRAVSIPVTCDSEGGYADDPATVGDNIARII
                     DAGAVGINIEDGREAPEVLAAKIVAVRQAADRAGVALFINARTDVYLKKLVEPEAAVA
                     ETLRRAALYRDAGADGLFAPGVVIPAEISAI"
     gene            complement(635..922)
                     /locus_tag="PLO65_08160"
     CDS             complement(635..922)
                     /locus_tag="PLO65_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRD28258.1"
                     /translation="MARKVSAKTIKGTLPTATPPEAAAFPAPETVAVRSHRIACDGVG
                     GALGHPRVWLEMGAAGFVDCPYCDRRFVAVTAEGDEDERLAPGVYEGAAGH"
     gene            1027..1989
                     /locus_tag="PLO65_08165"
     CDS             1027..1989
                     /locus_tag="PLO65_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD28259.1"
                     /translation="MDLPDYAIEATGLVKTYAATKTTPEKLALNGIDLKIPRGSIFGL
                     LGPNGAGKSTFINILAGLTRKTAGEVKIWGRDIDTRPRDARAAIGVVPQELAADVFFT
                     PRESLEVQAGMYGVPRKERRTDELLAALGLSDKADAYVRQLSGGMKRRLMVAKAMVHN
                     PPVLILDEPTAGVDVELRRQLWNYVLGLNRQGVTIVLTTHYLEEAQELCDQIAIVNRG
                     EVAACEPTATLLQRLDTRNVVVTPEEPVATAPDLTGFETRLRSGGSFAVTYKTGQSSV
                     EQVLAAVRASGLHIKDIATEDPDLEDVFLALTYEAPGGLDPTKD"
     gene            complement(2124..2306)
                     /locus_tag="PLO65_08170"
     CDS             complement(2124..2306)
                     /locus_tag="PLO65_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRD28260.1"
                     /translation="MADAAPYKTWQCRTCGFLYDEAEGDAGEGLAPGTRWADIPDSWV
                     CPLCGTAKADFDMVEL"
     gene            complement(2309..2956)
                     /locus_tag="PLO65_08175"
     CDS             complement(2309..2956)
                     /locus_tag="PLO65_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28261.1"
                     /translation="MAQSAPVEHRTRLTQAERRDQSERGLLRAATEVIAERGVTAATF
                     DAIGTRAGYSRGLATQKFGSKQGLIEALIAHLHARQDEVLGALGVDAMPGLDAVLTYV
                     DLYLRDLALKGENRAYFMLLAGAVADLSALRAAFAASHERVERRLEAMVQRGQAQGAI
                     RRDLDADAAALMVGSLLLGLSIQCLIDPDMDLEPIRATSLATLRLSFAAPALPEQ"
     gene            3062..4336
                     /locus_tag="PLO65_08180"
     CDS             3062..4336
                     /locus_tag="PLO65_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD28262.1"
                     /translation="MMIPQDLANTIVDPKAYADGKRIDDAFRWLRANAPLAKAQPEGF
                     DPFWVVTRFADIQEIEKQNDLFHNGDLATVVTPIVMDQKVRQMTGGSPHLVRSLVQMD
                     NPDHMNYRRLTQAWFLPQNIRGLEARIREIARGFVDRMAAKGDRCDFARDVAFLYPLH
                     VIMEVLGVPEIDEPRMLKLTQELFGSADPELNRSGSAVTDADAGLAAVQATVMDFMTY
                     FNAMTEDRRANPRNDLASVIANGQIEGKPLGHLEAMSYYIIAATAGHDTTSNTTSGAM
                     WALAQNPDQFAKVKADLSLIPGLVEESIRWETPVKHFMRSATADAEVAGQKIARGDWI
                     MLSYPSGNRDEAVFKDPFRFDIERSPNKHVAFGYGAHVCLGQHLGRMEMRVLWEELLP
                     RLESVELDGEPKRMEAAFVCGPKSVPIRFRMH"
     gene            4417..5736
                     /locus_tag="PLO65_08185"
     CDS             4417..5736
                     /locus_tag="PLO65_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRD28263.1"
                     /translation="MLERLFRLKENGTAVRTEVLAGLTTFLTMAYIILVNPTILADAG
                     MPMAGAAAATCLAAAFGSITMGLVANYPIALAPGMGLNAYFTYTVVKGMGLPWETALG
                     CVFLSGVLFLLLTVSGVRRLIVGAIPPSLYAAIAAGIGLFIAFIGLKGAGIVVASPAT
                     TVTLGDLTTPTAGLALFGLVLTGALLAWRIRAAMLVGILATTALAWAVGAVHWTPVDY
                     SLEALTATAFRLDVPGALGVGGQGAALALVEIVVVFLFVDLFDNVGTLVAVTGRAGLI
                     RPDGTIPRLNRILLSDSAAAMVGAVAGTTTTTSYIESAAGVAAGGRTGLTAVVVGLLF
                     LVALVFAPFAQVIPAAATAPALILVGALMMAPLAAADWEDPLVAIPGFLTLVAIPLTF
                     SIANGLAFGIIAHAVLKLARGQARPADWMLYLLAALFTARFIWLAAG"
BASE COUNT          853 a         2105 c         1993 g          864 t
ORIGIN      
        1 cgatggcgct gatttcggcc gggatgacca cgcccggcgc gaacaggccg tcggcccccg
       61 cgtcgcgata gagggccgcg cggcgcagcg tctcggcgac ggccgcctcg ggctcgacca
      121 gcttcttgag atagacgtcc gtgcgcgcgt tgatgaacag cgccaccccg gcgcggtcgg
      181 cggcctggcg cacggcgacg atcttggccg ccagcacctc gggggcctcg cggccgtcct
      241 cgatgttgat gcccacggcg ccggcgtcga tgatccgggc gatgttgtcg ccgaccgtcg
      301 cgggatcgtc ggcgtagccg ccctcgctgt cgcaggtgac cgggatcgac accgcgcggg
      361 tgatctcggc cacggtccgg accagcagct cgatcggcag gtgatggccg tcggcgtagc
      421 cgtggctcca gcagacggcc gcgctggagg tggccacggc cggcgcgccg agggtctcga
      481 tgatgcggca gctgccggcg tcccagccgt tggcgaggat cagcaggtcg gggcctgaat
      541 gcaggcggcg gaaggtgtct gcggtcgtgt tcatggaccc agactaggcc gccggagccg
      601 atcggtcagc cccgccgatg gccggatccg gacctcaatg gccggccgcg ccttcgtaga
      661 cgcccggggc caggcgttcg tcctcatcgc cctcggcggt cacggccacg aagcggcggt
      721 cgcagtaggg gcagtcgacg aagccggccg cgcccatctc gagccagacg cgcggatggc
      781 ccagggcccc gccgacgccg tcgcaggcga tgcggtgcga gcgcacggcc acggtctcgg
      841 gggccgggaa ggccgcggcc tccgggggcg tggcggtggg cagggtcccc ttgatcgtct
      901 tcgccgaaac cttgcgcgcc atcattctct tcccattgcc gcccttggct acgcggcccg
      961 tggcgcatac ctatgcgagg ccccgccggg gcgtcaattg atccagagct ccccaagaaa
     1021 gcccgcatgg acctgcccga ctacgccatc gaggccaccg gcctggtgaa gacctacgcc
     1081 gccaccaaga ccacgcccga aaagctggcc ctcaacggca tcgacctgaa gataccgcgc
     1141 ggctcgatct tcggcctgct ggggcccaac ggggccggca aatcgacctt catcaacatt
     1201 ctcgccggcc tgacgcgcaa gaccgcgggc gaagtgaaga tctggggccg cgacatcgac
     1261 acgcggccgc gcgacgcccg cgccgccatc ggggttgtgc cgcaggagct ggccgccgac
     1321 gtgttcttca ccccgcgcga atcgctcgag gtccaggccg gcatgtacgg cgtgccgagg
     1381 aaggagcgcc gcaccgacga gctgctggcg gccctcgggc tcagcgacaa ggccgacgcc
     1441 tacgtccgcc agctgtccgg cggcatgaag cgccggctga tggtcgccaa ggcgatggtg
     1501 cacaacccgc cggtgctgat cctcgacgag ccgaccgccg gggtcgacgt cgagctgcgc
     1561 cggcaactgt ggaactacgt cctgggcctg aaccgccagg gcgtgaccat cgtcctgacc
     1621 acccactacc tcgaggaagc gcaggagctc tgcgaccaga tcgccatcgt caaccgcggc
     1681 gaggtggcgg cctgcgagcc gaccgcgacc ctgctgcagc ggctggacac ccgcaacgtg
     1741 gtggtgacgc ccgaggagcc ggtcgccacg gcccccgacc tgaccggctt cgagacccgg
     1801 ctgcgcagcg gcggctcgtt cgcggtgacc tacaagaccg gccagtcgag cgtcgagcag
     1861 gtgctggccg ccgtgcgcgc cagcggcctg cacatcaagg acatcgccac cgaggacccc
     1921 gacctagagg acgtcttcct ggccctgacc tacgaagccc ctggcggcct ggacccgacg
     1981 aaggactgag gggcgcgcgc gccggcctcg ggcggggaag gcctcgcaac ggtcctgccc
     2041 tgatgtcttg aaggccagag ggtcagtcta catccgcgca ggaatgcagg gcgggatggc
     2101 cgcgaggcat tccccccctg accctacagc tcgaccatgt cgaagtcggc cttggccgtg
     2161 ccgcacaacg ggcagaccca gctgtcggga atgtcggccc agcgcgtgcc gggggccagg
     2221 ccctcgcccg cgtcgccctc ggcctcgtcg tagaggaagc cgcaggtgcg gcactgccag
     2281 gtcttgtacg gcgcggcgtc ggccatggtc attgctccgg gagggcgggc gcggcgaagc
     2341 tgagccgcag ggtcgccagg ctggtggcgc ggatcggctc cagatccatg tccggatcga
     2401 tcaggcactg gatcgacagg ccgagcagca ggctgccgac catcagggcc gcggcgtcgg
     2461 cgtcgagatc gcgacggatc gcgccctgcg cctggccgcg ctggaccatc gcctcgagcc
     2521 ggcgctcgac ccgttcatgc gaggccgcga aggccgcgcg cagggccgac aggtcggcga
     2581 cggccccggc cagcagcatg aagtaggcgc ggttctcgcc cttcagcgcc aggtcgcgca
     2641 ggtacaggtc gacataggtc agcaccgcgt ccagcccggg catggcgtcg acgcccagcg
     2701 cccccagcac ctcgtcctgg cgcgcgtgca gatgggcgat cagggcttcg atcagaccct
     2761 gcttggagcc gaacttctgc gtcgccaggc cgcggctgta gccggcccgg gtcccgatcg
     2821 cgtcgaaggt ggccgccgtc acgccgcgct cggcgatcac ctcggtcgcg gcccgcagca
     2881 ggccgcgctc ggactggtcg cggcgctccg cctgggtcag gcgggttcgg tgttcgacgg
     2941 gcgcggactg agccatgtca attctctaac ttacttgttg gccgtatagc aagtgcggtg
     3001 ctagcctgtt ccggaagccg ccggtccacg ggcggcgcgg aaccgctcag gaagacatgc
     3061 gatgatgatt ccccaggacc tggccaacac cattgtcgat cccaaggcct acgccgacgg
     3121 caagcggatc gacgacgcct tccgctggct gcgggccaac gcgccgctcg cgaaggccca
     3181 gccggaaggc ttcgatccct tctgggtggt cacccgcttc gccgacatcc aggagatcga
     3241 gaagcagaat gacctgtttc acaacggcga cctggccacc gtggtgaccc ccatcgtgat
     3301 ggaccagaag gtccgtcaga tgaccggcgg ctcgccgcac ctggtccgct cgctggtcca
     3361 gatggacaac cccgaccaca tgaactaccg gcgcctgacc caggcctggt tcctgccgca
     3421 gaacatccgc ggcctcgagg cccgcatccg cgagatcgcc aggggcttcg tcgaccgcat
     3481 ggcggccaag ggcgaccgct gcgactttgc ccgcgacgtg gccttcctct acccgctgca
     3541 tgtgatcatg gaggtcctcg gcgtgccgga gatcgatgag ccgcgcatgc tcaagctgac
     3601 ccaggaactg ttcggcagcg ccgacccgga actgaaccgc agcggctcgg ccgtcaccga
     3661 cgccgatgcc ggccttgccg ccgtgcaggc gacggtgatg gacttcatga cctatttcaa
     3721 cgccatgacc gaggatcgcc gggccaatcc gcgcaatgat ctggccagcg tcatcgccaa
     3781 cggccagatc gagggcaagc cgctcggcca cctggaggcg atgagctact acatcatcgc
     3841 cgccaccgcc ggtcacgaca ccacctccaa caccacctcc ggcgccatgt gggccctggc
     3901 ccagaacccg gaccagttcg ccaaggtgaa ggccgacctg tcgctgatcc cgggcctggt
     3961 cgaggaatcg atccgctggg aaaccccggt gaagcacttc atgcgcagcg ccacggccga
     4021 cgccgaggtc gccggccaga agatcgccag gggcgactgg atcatgctca gctacccgtc
     4081 gggcaaccgc gacgaggcgg tgttcaagga cccgttcagg ttcgacatcg agcgctcgcc
     4141 caacaagcac gtggcgttcg gctacggcgc ccacgtctgt ctcggccaac acctcggccg
     4201 catggagatg cgcgtgctgt gggaggagct gctgccgcgc ctggagagtg tcgaactgga
     4261 cggcgagccg aagcgcatgg aggccgcctt cgtctgcgga cccaagtcgg tgccgatccg
     4321 cttcaggatg cactgacccc gcgcgggcgg cggctgtttg ccgccgcgcc ccgccgggcc
     4381 taggctggtc ccccggtacg gaagaggggc gggccgatgc tggagcgtct gttcaggctg
     4441 aaggaaaacg gcacggccgt gcgcaccgag gtgctggccg ggctgaccac cttcctgacc
     4501 atggcctaca tcatcctcgt caacccgacg atcctggccg acgccggcat gccgatggcc
     4561 ggggcggcgg cggccacctg cctggcggcg gcgttcggct cgatcaccat gggcctggtc
     4621 gccaactacc cgatcgcgct ggcgccgggc atggggctga acgcctactt cacctatacc
     4681 gtggtcaagg gcatgggcct gccgtgggag acggcgctgg gctgcgtgtt cctgtcgggc
     4741 gtgctgttcc tgctgctgac cgtctccggc gtgcggcggc tgatcgtcgg ggccatcccg
     4801 ccctcgctct acgccgccat cgccgccggc atcgggctgt tcatcgcctt catcgggctg
     4861 aagggcgcgg gcatcgtcgt ggccagtccg gcgaccacgg tgaccctcgg cgacctgacc
     4921 acccccacgg ccggcctggc cctgttcggc ctggtgctga ccggcgccct gctggcctgg
     4981 cgcatccgcg ccgccatgct ggtcggcatc ctcgccacca cggccctggc ctgggccgtc
     5041 ggcgccgtgc actggacgcc ggtcgactac agtctcgagg ccctgaccgc gaccgccttc
     5101 cgcctcgacg tccccggcgc cctgggcgtc ggcggccagg gcgcggccct ggccctggtc
     5161 gagatcgtcg tggtgttcct gttcgtcgac ctgttcgaca acgtcggcac cctggtggcg
     5221 gtgaccggtc gcgcggggct gatccggccg gacgggacca ttccgcggct caaccgcatc
     5281 ctgctgtccg attccgccgc ggcgatggtc ggcgccgtgg ccgggaccac caccaccacc
     5341 agctatatcg agagcgccgc gggcgtcgcc gccggcggcc gcacaggcct gacggcggtg
     5401 gtcgtgggcc tgctgttcct cgtcgccctg gtcttcgccc cgttcgccca ggtgatcccc
     5461 gcggcggcca ccgccccggc cctgatcctg gtcggagccc tgatgatggc cccgctggcg
     5521 gccgccgact gggaggatcc gctggtcgcc atccccggct tcctgaccct ggtcgccatc
     5581 ccgctgacct tctcgatcgc caacggcctg gctttcggca tcatcgccca tgcggtgctc
     5641 aagctggccc ggggccaggc ccgaccagcc gactggatgc tctacctgct ggcggcgctt
     5701 ttcacggccc ggttcatatg gctggcggcg ggctagcgcc gcgccgatct ttccgctatt
     5761 gacccgcctc cgccagcgat ccgcacccgt agctcagctg gatagagcgt tgccc
//
LOCUS       DAORNX010000155         5794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10232_length_5794_cov_7.590521, whole genome shotgun sequence.
ACCESSION   DAORNX010000155 DAORNX010000000
VERSION     DAORNX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_5794_cov_7.590521"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLO65_08190"
     CDS             <1..859
                     /locus_tag="PLO65_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28264.1"
                     /translation="SGRLRVVASGERRIAVTDYGPRSGKPVIVMHGAATGRRLPPAMV
                     VAMHARGLRPLVPQRPGYGLTDVADQPYLEQCASDMARLMDALRIDQARLLVRDSATP
                     AGLLFANQHPDRVASGVIVNPKWPLRALSHSAPRPTSLMAALGNLFVESPQLIGLVGE
                     ILRRQTRTEMLEKVMRQSLAHLPCDQETLREPGVLDALVQDAQGMFARSSMGFTSEHM
                     AFVTGWDLPETVGGARWTVAECGQLTLPDAMSVWGRLPNATFQVIPNAGLLVYFTHPE
                     VVADLVAGT"
     gene            1110..2075
                     /locus_tag="PLO65_08195"
     CDS             1110..2075
                     /locus_tag="PLO65_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRD28265.1"
                     /translation="MRKIVDGVVGLALAAMVMAPASPAMAQYGMDKKKPANAATPEMP
                     RCDRPLGVIAVQEPDNRWWTELGLSSPEVLIKLFASRSGCFRVVDRNAGLAMRGQERD
                     LAGSGDLQRGSNIGRGQVVAADFFLVPDIANSNSNSGGNAIGAIAGSVIPGGFGALAG
                     QIRTKKSEAQVIITVVDARTTEQLYVAEGTAKKTDIGFGVGGGALGSTGFGAAAGGGY
                     SNTEIGKVITAAYFNAFIELIHYAQSGAMPTGAEASGNAGQAAVVTTQSVPMRSGPSP
                     TASLVLSVAAGAILYPTGGRNGVWMEVDDEHGNRGWVSSAFVTAR"
     gene            complement(2142..3353)
                     /locus_tag="PLO65_08200"
     CDS             complement(2142..3353)
                     /locus_tag="PLO65_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28266.1"
                     /translation="MDLAFSDDDKAFQREVRDWIAENYDDDLRRQMSQSKNGYLDKAG
                     QVKWQKRLASKGWATTNWPVEYGGPGFTPSQAYIFNMEMSLAGVPTASPMGLKMVAPV
                     IMAFGTPEQKAQHLPPIINSDIWWCQGYSEPGSGSDLASLQMKAERDGDDYVLNGSKI
                     WTTHAQWADWMFCLVRTSFDGKPQNGISFLLLRMDSPGITVKALPTLDGPAEGQQEIN
                     QVFFDNVRVPVANRIGEEGKGWTYAKYLLEFERGNAYAPGLMNALQKVRKIASAELGD
                     SGEPLIRDRDFQRKLAEMEIRIEALNATELRIFSAMGSGQPVGPASSMLKCAGSEAQQ
                     AITELAVEAVGGYAAPFVQDTWAVTNAPRPGPDYAAPAAPSYFNYRKASIYAGSNEIQ
                     RNIMAKLVLGL"
     gene            complement(3359..3646)
                     /locus_tag="PLO65_08205"
     CDS             complement(3359..3646)
                     /locus_tag="PLO65_08205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRD28267.1"
                     /translation="MIGVVATLTAAEGKGPDLEKVFADLAAKVKANEPGCLMYQLTRS
                     RTEPNVYKVLEMYASQDALTAHGQTEYFKAAGPGIGACLGGRPSIEYLDAV"
     gene            complement(3849..4145)
                     /locus_tag="PLO65_08210"
     CDS             complement(3849..4145)
                     /locus_tag="PLO65_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28268.1"
                     /translation="MDKLNSAGGSRSAFKGLAWLAGALATCAALAVGAVLAVFAAATV
                     VLIALMSSALLALAGVAVRARRSVRTRQSGDTDIIEARNVGGHSWVAYGWNERR"
     gene            complement(4257..4952)
                     /locus_tag="PLO65_08215"
     CDS             complement(4257..4952)
                     /locus_tag="PLO65_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family molecular chaperone"
                     /protein_id="HRD28269.1"
                     /translation="MSFWRTIAGLAARRVDDAECPDCPPGPPGEDPAFTTAVTALGAK
                     LAKADGRADPDEFDAFSTVFQPDSGSERNVHRLYDLARQTTRGFESYARRLAKRYRSC
                     PQLLEDVLDGLFHIAGSDGAVTADELEYLETVSDLFGFSPLTFRRLKATHLGAGPDDP
                     YVILGVLPDAADEVVRAAWRTQVSSAHPDRARARGLPSEFIEVAETKTASINAAFDAV
                     MKERRELSLVGAV"
     gene            4988..>5794
                     /locus_tag="PLO65_08220"
     CDS             4988..>5794
                     /locus_tag="PLO65_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HRD28270.1"
                     /translation="MTTGGYQTSGWTFWIDRGGTFTDVIGRAPDGAEQTLKLLSASPS
                     YADAAVEAMRRLLGVEPDAPFPAARVAAIRMGTTVATNALLERKGARTLLVTSRGFGD
                     SVVIGDQSRPELFALDIIRPDPLYSGVVEAGERLAADGAVVAPLDVDTLAADLAWARA
                     DGFASVAIAFLHSDLNPDHERTAGEIAWAAGFDYVALSHEVSPLPRFTPRAETTVADA
                     YLTPVLRAYVDQVARAVGGAPLWFMTSAGALIRAQAFRGKHQGDAGFGGAG"
BASE COUNT          892 a         1969 c         2030 g          903 t
ORIGIN      
        1 gagcgggcgt ttgcgggtcg tggcgtctgg cgagcggcgc atcgccgtga ccgactatgg
       61 tccgcgcagc ggcaagccgg tgatcgtcat gcacggggcc gcgacgggcc gccggctgcc
      121 tccggcgatg gtcgtggcca tgcatgcgcg cggcctgcgc ccgctggtgc cgcagcggcc
      181 gggctacggc ctgacagacg tcgccgacca gccctatctg gagcaatgcg ccagcgacat
      241 ggcccggctc atggacgccc tgcgcatcga ccaggcccgc ctgctggttc gcgattccgc
      301 cacgccggcg ggcctcttgt tcgccaacca gcaccccgat cgcgtggcct ccggcgtaat
      361 cgtcaacccc aagtggccgc tccgggccct gtcgcacagc gcgccgcggc cgacctcgct
      421 catggcggcc ctcggcaatc tgttcgtcga gagtccgcag ctgatcggac tggtcggcga
      481 gatcctgcgc cgtcagaccc gcaccgagat gctcgaaaag gtcatgcgtc agtcgctggc
      541 gcacctgccc tgcgaccagg agaccctgcg cgagcccggc gtgctcgacg cgctggtcca
      601 ggacgcccag ggcatgttcg cccgctcgag catgggcttc acgtccgagc acatggcgtt
      661 tgtcaccggg tgggacctgc cggaaaccgt gggaggcgca cgctggaccg tggccgagtg
      721 cggacagctg acgcttcccg acgccatgtc ggtctggggc cggctgccca acgcgacctt
      781 ccaggtcatc cccaatgccg gcctgctggt ttatttcacc catccggagg tggtcgccga
      841 tctggtggcg gggacctgac gggtcccccc aaaaggggga ggccggcccg gcgaggtcgc
      901 cccagactgg cgaaatgcgt cgggcctctg gcgctccgcg attcgccgcg acacagtcct
      961 gtcgtcgcgc agtgagggcg acggcgtccg gacgatgaga acgaagaaca ggtcaggtcg
     1021 gggtcagatc aaggcgccgc cgggccggca tcacgtagcg acgggcgggg cgtaggcgcg
     1081 ccgaacggaa gacatctgga gggaagaaga tgcgcaagat agtggacggc gtcgtgggcc
     1141 tggccctggc cgccatggtc atggctccgg cgagcccggc catggcccag tacgggatgg
     1201 acaagaagaa gccggccaac gccgcgacgc ccgagatgcc gcggtgcgac cgcccgctcg
     1261 gcgtcatcgc cgtgcaggag cccgacaatc gctggtggac cgagctgggc ctgtcgagcc
     1321 ccgaggtcct gatcaagctg ttcgcctcac ggtcgggctg cttccgggtc gtcgaccgaa
     1381 acgccggcct ggcgatgcgc ggacaggaac gtgaccttgc cggctccggc gaccttcagc
     1441 gcggctccaa catcggtcgt ggccaggtgg tcgccgccga cttcttcctc gtgccggaca
     1501 tcgccaactc caattccaac agcggcggca acgccatcgg ggcgatcgcc ggcagcgtga
     1561 tccccggcgg cttcggcgcc ctggccggcc agatccggac caagaagtcc gaggcccagg
     1621 tgatcatcac cgtcgtcgac gcccgcacca ccgaacagct ctatgtcgcc gagggcacgg
     1681 ccaagaagac cgacatcggc ttcggcgtcg gcggcggcgc cctgggcagc acgggcttcg
     1741 gcgcggcggc cggcggcggc tacagcaaca ccgagatcgg caaggtcatc acggccgcct
     1801 acttcaacgc cttcatcgag ctgatccact acgcccagtc gggcgccatg ccgaccggcg
     1861 ccgaagcctc gggcaacgcc ggccaggcgg ccgtggtgac gacccagtcg gtgccgatgc
     1921 ggtcgggacc gagcccgacg gccagcctgg tgctgtcggt cgcggccggc gccatcctct
     1981 acccgaccgg cggccgcaac ggcgtctgga tggaagtgga tgacgaacac ggcaaccgtg
     2041 gctgggtgtc gtcggccttt gtgacggcgc gataacgcca caacgacgaa gggcggagcg
     2101 ccaggcgctc cgccccattt ctcagggtcc gccggacggg atcagagacc cagcaccagc
     2161 ttggccatga tgttgcgctg gatctcgttc gagccggcgt agatcgaggc cttgcgatag
     2221 ttgaagtagc tgggcgccgc cggcgcggcg tagtcgggcc cgggccgcgg cgcgttggtc
     2281 accgcccagg tgtcctggac gaagggcgcc gcatagccgc cgaccgcctc caccgccagt
     2341 tcggtgatgg cctgctgcgc ttcagagccg gcgcacttga gcatcgacga ggccggcccg
     2401 accggctgcc ccgaccccat ggccgagaaa atccgcagtt cggtggcgtt gagcgcctcg
     2461 atacggatct ccatctcggc cagcttgcgc tggaaatccc ggtcgcggat caatggctcg
     2521 ccggaatccc cgagctcggc cgaggcgatc ttgcgcacct tctgcagcgc gttcatcagg
     2581 cccggcgcgt aggcgttgcc gcgttcgaac tcgagcaggt atttggcgta ggtccagccc
     2641 tttccctcct cgccgatgcg gttggcgacc ggcacgcgga cattgtcgaa gaaaacctgg
     2701 ttgatctcct gctggccctc ggccggcccg tcgagggtcg gcagggcctt gaccgtgatg
     2761 cccggcgaat ccatccgcag caggagaaag ctgatgccgt tctgcggctt gccgtcgaac
     2821 gaggtgcgca cgaggcagaa catccagtcg gcccactggg cgtgggtggt ccagatcttc
     2881 gagccgttga ggacgtagtc gtcgccgtcg cgctcggcct tcatctgcag gctggcgagg
     2941 tcggacccgg agccgggctc ggaataaccc tggcaccacc agatgtcgga attgatgatc
     3001 ggtggcaggt gctgggcctt ctgttcgggc gtgccgaagg ccatgatcac cggcgcgacc
     3061 atcttcaggc ccatcggcga ggcggtcggc acgcccgcga ggctcatctc catgttgaag
     3121 atgtaggcct gtgacggggt gaaaccgggt ccgccgtact cgaccggcca gttggtcgtc
     3181 gcccagccct tcgaggccag gcgcttctgc catttcacct ggccggcctt gtcgagataa
     3241 ccgttcttcg actggctcat ctgccggcgc aggtcgtcgt cgtagttttc ggcgatccag
     3301 tcgcggacct cgcgctggaa ggccttgtcg tcatccgaga acgcgaggtc catggggatc
     3361 agaccgcgtc gagatattcg atcgagggac ggccgcccag gcaggcgccg atgcccggtc
     3421 cggcggcctt gaagtactcg gtctgaccat gcgctgtcag ggcgtcctgc gaggcgtaca
     3481 tctccagcac cttgtacacg ttgggctcgg tgcggctcct ggtcagctgg tacatcaggc
     3541 agccgggctc gttggccttc accttggccg cgaggtccgc gaacaccttc tccaggtcag
     3601 ggcccttgcc ctcggccgcc gtcaaggtcg ccactacgcc gatcatgttt ccgctcccga
     3661 acgtttgttt gaagcaggga caatggggcg gcattggcgc cggtgcaagc atgacgtgga
     3721 gggagggtgc gtggttcgac acgcgctttg cgccgctcac catgacgtgg ttcagcgtca
     3781 ggcccggcac gtcatcctga gcaggcccga agggccgtgt cgaaggacgc actatgtcga
     3841 aatggagcct agcgccgctc gttccagccg taggcgaccc aggagtggcc gccgacgttg
     3901 cgggcctcga tgatgtcggt gtcgccactc tggcgggtgc ggacgctgcg gcgggcgcgc
     3961 acggcgacgc cggccagggc cagcagggcg ctggacatca gggcgatgag gacgacggtc
     4021 gcggcggcga acaccgccag cacggcgccg accgccaggg ccgcgcaggt ggccagggcg
     4081 ccggccagcc aggccaggcc cttgaaggcg gaacgagaac cccccgcaga gttcagtttg
     4141 tccatcttgt cctcgacggg ccgcgagagg cgcggccacg gatcactatc ttgtcgttaa
     4201 ccgctgaacg tcaactgaac acgcgcgata cgggcgcaga aatcggtcct gcccgttcag
     4261 acggcgccga ccagcgacag ctcgcggcgc tccttcatga ccgcgtcgaa cgcggcgttg
     4321 atcgaggcgg tcttggtctc cgccacctcg atgaattcgc tgggcagccc gcgcgcgcgg
     4381 gcccggtcgg ggtgagctga agacacctgc gtgcgccagg cggcccgcac gacctcgtcg
     4441 gccgcgtccg gcaggactcc gaggatcacg tagggatcgt ccggccccgc gcccaggtgc
     4501 gtggccttga gccgccggaa ggtcagcggc gagaagccaa acaggtccga gaccgtctcg
     4561 aggtattcaa gctcgtcggc ggtcaccgcg ccgtctgagc ctgcgatatg gaacaggccg
     4621 tccagcacat cctcgagcaa ctgcgggcag ctgcggtagc gcttggccag ccgccgggcg
     4681 tagctttcaa agccccgcgt cgtctgccgg gccagatcat acaggcggtg gacgttgcgt
     4741 tccgaccccg agtcgggctg gaacacagtg gagaaggcgt cgaattcgtc ggggtcggcg
     4801 cgaccgtcgg ccttggccag cttggccccg agcgccgtaa cggcggtcgt gaacgcgggg
     4861 tcctcacccg gcggaccggg aggacagtcg gggcattcgg cgtcgtcgac gcgacgggcc
     4921 gccaagcctg ctatggtgcg ccagaaactc atgggatcga tttgcagctt atgatcgtgg
     4981 cgccggcatg acaacggggg gttaccagac gtcgggctgg acattctgga tcgatcgcgg
     5041 cggcaccttc accgacgtga tcggccgcgc gcccgatggc gcggaacaga cgctaaagct
     5101 tctgtcggcg tcgccctcct atgccgacgc ggccgtggag gccatgcgcc gcctgctggg
     5161 ggttgaaccc gacgcaccgt ttcccgccgc gcgggtcgcg gcgatccgca tgggcaccac
     5221 ggtggccacc aacgccctgc tggaacgcaa gggcgccagg acgctgctcg taaccagccg
     5281 gggcttcggc gactccgtgg tcatcggcga ccagtcgcgg ccagagctgt tcgccctcga
     5341 catcatcagg cccgaccccc tctacagcgg cgtcgtcgag gccggcgagc gactggcggc
     5401 ggacggcgcg gtcgttgcgc cgctggacgt cgacaccctg gcggcggacc tggcctgggc
     5461 gcgagccgac ggcttcgcca gcgttgcgat cgccttcctg cacagcgacc tgaatcccga
     5521 tcacgaacgg acggcgggcg agatcgcttg ggcggcaggg ttcgactatg tggccctgag
     5581 ccacgaggtc agccccctgc cccggttcac gccgcgcgcc gagacgacgg tggccgacgc
     5641 ctacctgacg ccggtgctgc gggcctatgt cgatcaggtc gcgcgggccg tcgggggcgc
     5701 gcccctgtgg ttcatgacct cggccggggc gctgatccgg gcgcaggcct tccgcggcaa
     5761 gcatcagggc gacgcgggct tcggcggcgc cggc
//
LOCUS       DAORNX010000156         5780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10256_length_5780_cov_7.481921, whole genome shotgun sequence.
ACCESSION   DAORNX010000156 DAORNX010000000
VERSION     DAORNX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10256_length_5780_cov_7.481921"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLO65_08225"
     CDS             complement(<1..189)
                     /locus_tag="PLO65_08225"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28271.1"
                     /translation="MGQVSGKTALVTGGSSGIGAACAETLAREGAKVLVTAGANGIGR
                     AIARAFAAEGARVHVCDVD"
     gene            complement(208..363)
                     /locus_tag="PLO65_08230"
     CDS             complement(208..363)
                     /locus_tag="PLO65_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD28272.1"
                     /translation="MPVSPDWNLYASFRGVMRHGSLSAAARALGLTQPTVGRHVAALE
                     AALRGQA"
     gene            570..>947
                     /locus_tag="PLO65_08235"
     CDS             570..>947
                     /locus_tag="PLO65_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28273.1"
                     /translation="MTGAEKFSVDPALPGSPLELYHMPFTHSLPGALAWSVGGALLAR
                     WALKTPWRASALVGAAVFSHWLLDFLVHRPDLELWFGGEKVGLGWWNWPVPEATLEMG
                     LLAAAGAWWAWRRAKEGLGVWPAA"
     assembly_gap    948..957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1178..2980
                     /locus_tag="PLO65_08240"
     CDS             1178..2980
                     /locus_tag="PLO65_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRD28274.1"
                     /translation="MNAPLKSPARDAAPPEWSLSDLYESRTDPRIEQDLARARRANDA
                     LVALKGRFVAGRADPGALGALIDEGISLYEEATNALWAVGAYASLAASTARDDPEWAR
                     FEGDLRTRASQIAAESLFFTLELNELEDAELEAALDAHLGAARWRPWIRRLRLSRPHE
                     LSPDLERLLIDRGPAVANWSRLYDETLAKLTAKVGREYLTLPEALNRLSDPSAARRKA
                     AANGLAEALHDRTPTMALVMNTLAFEKQVEDRWRRYSYPAQSRHIGNEVDAEAVEAMV
                     EAVTDGYAATSHRYYAAKAKVMGRRTLDYWDRNAPLDAAAPRQYGWDEAKGVVLESFS
                     ALAPKFADQARTFFASPWIDARPRPGKQSGAYSHPVTAEKHPYVFLNFMGERRDVLTL
                     AHELGHAVHQTLCAPLGTLLADTPLTLAETASIFGEGLVFERLLAEAAPADRRALLAG
                     KIEDGLNTVVRQIAFHQFETRFHDARQDGELSPDEIGALWLDVMGDSLGPAIKLNPGY
                     EHYWAYVSHFVHAPFYVYAYAFGDLLVRALMEKRREDPAGFAPLYETLLAAGGTKTYV
                     EALAPFGLDPREKSFWAAGMRDIARLVEEFDALT"
     gene            3050..3523
                     /locus_tag="PLO65_08245"
     CDS             3050..3523
                     /locus_tag="PLO65_08245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD28275.1"
                     /translation="MSDDLTEAQTGDIPPGYSLLNWTRGFGRQIGPLYEKYEAGGCTL
                     AFRVEEHHTNGMANCHGGMLMSFADMAWGRVISVQKSHFWVTVRLTCDFLSAAKLGDW
                     VEGSAEMIADEDSVFTVRGRIWSGDRTLMTGTGVFKALGPRPPRPGEKAYEAAHA"
     gene            3516..4454
                     /locus_tag="PLO65_08250"
     CDS             3516..4454
                     /locus_tag="PLO65_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28276.1"
                     /translation="MPDDQKPWPEFPAPLAAFNGDKPPAPAWFEAALADAPERSTFTF
                     EGADIELLTWGEIGKPGLLLLHGNGASADWWTFIAPWFAKDWRVAAFSWSGMGRSDWR
                     ETYTAEQFAQESFAAAEAAGLYAAGKPVFVAHSFGGFPTMLAASKYGDRLGAVVLVDS
                     MVRPPELEWRGPPKRVSPNRVYPTLTEALGRFRLAPPQGCENPYIADWIARNALKEVE
                     GGWTWRFDPFMWSSFMMEDRGPLLANITCPVAMMWGDRSRLAEPHILDYMRGLLPPGS
                     PQIAIPDADHHVMIDQPLAFVGALRGLLAGWPGERA"
     gene            4626..4856
                     /locus_tag="PLO65_08255"
     CDS             4626..4856
                     /locus_tag="PLO65_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HRD28277.1"
                     /translation="MASEYHRGEMDISEQTATYHAVMGMTKWGSLALAVAILMLTLWF
                     CTPAGFFGGLVPGLIVLVAGVALLREKKGAGH"
     gene            4990..>5780
                     /locus_tag="PLO65_08260"
     CDS             4990..>5780
                     /locus_tag="PLO65_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRD28278.1"
                     /translation="MPVIAVTRERRAGETRVAATPETVKKLIAAGWSLTVEAGAGTAA
                     SFPDADYAAAGATIARSLKDAVGKADVLFKVRGPEADEIAALKPGAMVVATLNPYQDR
                     ATLEGLAKAKASAFAMEFVPRITRAQVMDVLSSQANLAGYRAVIEASEAYGRAMPMMM
                     TAAGTVAAAKVFIMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVESLGAKFLAVE
                     DEEFRNAQTAGGYAKEMSKEYQAKQAELVSSHIAKQDIVITTALIP"
BASE COUNT          857 a         2106 c         1949 g          858 t
ORIGIN      
        1 atcgacgtcg cagacgtgca cccgtgcccc ctcggccgcg aaggcgcgtg cgatcgcccg
       61 tccgatgccg ttcgcgcccg ccgtcaccag caccttcgcg ccctcgcgcg ccaacgtctc
      121 ggcgcacgcc gcgccgatgc ccgaagaacc gcccgtcacc agcgcggtct tgcccgatac
      181 ctgtcccatc gttttccccg tttgttttca ggcctggccg cgcagcgccg cctcaagcgc
      241 cgccacatgc cggccgacgg tcggctgggt caggccgagc gcgcgcgccg ccgccgacag
      301 gctgccgtgg cgcatgaccc cgcgaaacga ggcgtagagg ttccagtcgg gcgaaacagg
      361 catgcaaata tgcatagcag accgagcatc atctacaatt gtcgaatatc ctgggcggag
      421 ggacaagggc ggtctcaaag cgaggagacc cccatgtttg tcggccacta cgccgccgcc
      481 cttgccgccc gcgcggccga accccgcggg ccgctgtgga gctatgtcgc cgcctgccag
      541 ctgctcgaca tcggctgggg cggctgatca tgaccggggc cgagaagttc agcgtcgacc
      601 cggccctgcc gggcagcccg ctcgagctct accacatgcc gttcacccac agcctgcccg
      661 gcgccctggc ctggtccgtc gggggagcct tgctggcgcg gtgggcgctg aagacgccgt
      721 ggcgcgcctc ggccctcgtc ggcgcggcgg tcttctcgca ctggctgctc gacttcctcg
      781 tccatcggcc cgatctcgag ctgtggttcg gcggcgagaa ggtcggcctg ggctggtgga
      841 actggcccgt gcccgaggcg acgctcgaga tgggcctgct ggccgcggcc ggcgcctggt
      901 gggcgtggcg gcgggcgaag gaggggctgg gcgtctggcc ggccgcgnnn nnnnnnntgt
      961 tcctgctgct gctgaccggc gtgcagatcg gctcgaaact ggccccgttc gggtccgacc
     1021 cggtccagac cggcgccatg gccatcgccg tctacctcgc cgtcgccgct gtcgcctggc
     1081 tggtcgagcc gcggacgcga ccggcctgat cgctctccgt ttccacgaat ggggagggag
     1141 gttgcgggcc ctgatccacc ggtccatatc cgccgccatg aacgcgccgc tcaagtcccc
     1201 cgcccgtgac gccgctccgc cggagtggag cctgtccgac ctctacgaaa gccggaccga
     1261 cccccggatc gagcaggacc ttgcccgcgc caggcgggcc aatgacgcgc tggtggcgct
     1321 caagggccgc tttgtcgccg gccgggccga tcccggcgcg ctgggcgccc tgatcgacga
     1381 ggggatcagc ctctacgaag aggcgaccaa cgccctctgg gcggtcggcg cctatgcctc
     1441 gctggccgcc tcgacggcgc gggacgatcc ggagtgggcc cggttcgagg gcgacctgcg
     1501 gacccgcgcg tcgcagatcg cggcggaaag cctgttcttc accctcgaac tcaacgagct
     1561 tgaggacgcc gagcttgagg cggccctgga cgcccacctg ggcgccgccc gctggcgacc
     1621 gtggatccgc cgcctgcgcc tttcccgccc gcacgagctg tcgccggacc tggagcggct
     1681 gctgatcgac cgcggcccgg ccgtggccaa ctggtcgcgg ctttatgacg agaccctggc
     1741 gaaactcacg gcgaaggtcg gccgcgaata cctgaccctg cccgaagcgc tgaaccggct
     1801 gtcggacccg tcggcggccc ggcgcaaggc ggccgccaat ggcctggccg aggcgctgca
     1861 cgaccgcacc ccgaccatgg ccctggtgat gaacaccctg gccttcgaga agcaggtcga
     1921 ggaccgctgg cgccgctaca gctaccccgc ccagtcgcgg cacatcggca acgaggtcga
     1981 cgccgaggcg gtcgaggcga tggtcgaggc cgtcaccgac ggctacgccg ccaccagcca
     2041 ccgctactac gccgccaagg cgaaggtcat gggccgcagg accctcgact actgggaccg
     2101 caacgccccg ctcgacgcgg ccgcgccgcg ccagtacggc tgggacgagg cgaagggcgt
     2161 agtgctggag agcttttcgg ccctggcccc caagttcgcc gaccaggcgc ggaccttctt
     2221 cgccagcccc tggatcgacg cccggccccg gcccggcaag cagtctggcg cctacagcca
     2281 tccggtgacg gccgagaagc acccctacgt gttcctcaac ttcatgggcg agcgacgcga
     2341 tgtgctgacc ctggcccacg agctgggcca cgccgtgcac cagacactgt gcgcgccgct
     2401 cggcaccctg ctggccgaca cgccgctgac cctggccgag acggcctcga tcttcggtga
     2461 ggggctggtg ttcgagcggc tgctggccga ggccgcgccg gccgaccgcc gcgccctgct
     2521 ggccggcaag atcgaggacg gcctcaacac cgtggtgcgc cagatcgcct tccaccagtt
     2581 cgagacccgg ttccacgacg cccgccagga cggcgagctg tcgcccgacg agatcggcgc
     2641 cctgtggctc gacgtgatgg gcgacagcct tggcccggcg atcaagctga atccgggcta
     2701 cgagcactac tgggcctatg tcagccactt cgtgcacgcg cccttctatg tctacgccta
     2761 cgccttcggc gacctcctgg tgcgggccct gatggagaag cgccgcgagg acccggccgg
     2821 cttcgcgccg ctctatgaga cgctgctcgc ggcgggtggc acgaagacct acgtcgaggc
     2881 cctcgccccg ttcggcctcg atccgcgcga gaagagcttc tgggccgccg gcatgcgcga
     2941 catcgcccgc ctggtcgagg agttcgatgc gctcacctag atcctccccg gagcgaagcg
     3001 agggggagga acggttggaa tgttttcaaa cgaccgttag acttccgcca tgtccgacga
     3061 tctcaccgaa gcccagacgg gcgacattcc gcccggctac agtctgctga actggacccg
     3121 cgggttcggc cggcagatcg ggccgctgta cgagaagtac gaggccggcg gctgcaccct
     3181 ggccttccgc gtcgaggagc atcacaccaa cggcatggcc aactgccacg gcggcatgct
     3241 gatgagcttc gccgacatgg cctgggggcg ggtgatctcg gtgcagaaat cgcacttctg
     3301 ggtcaccgtc cggctgacct gcgacttcct gtccgccgcc aagctcggcg actgggtcga
     3361 gggctcggcc gagatgatcg ccgacgagga ctcggtgttc accgtgcggg gccgcatctg
     3421 gtcgggcgac cgcacgctga tgaccggaac cggcgtgttc aaggcgctcg ggccgcgccc
     3481 gccgcgtccg ggcgagaagg cctacgaggc cgcgcatgcc tgacgatcag aagccctggc
     3541 cggaattccc ggcgccgctg gccgcgttca acggcgacaa gccgccggcc cccgcctggt
     3601 tcgaggcggc gctggccgat gcgcccgagc gctcgacctt caccttcgag ggcgcggaca
     3661 tcgagctgct gacctggggc gagatcggca agcccggcct gctgctgctg catggcaacg
     3721 gcgccagcgc cgactggtgg accttcatcg cgccctggtt cgcgaaggac tggcgggtgg
     3781 cggcgttcag ctggagcggc atgggccggt ccgactggcg cgagacctac acggccgagc
     3841 agttcgccca ggagtcgttc gccgcggccg aggccgccgg cctctacgcc gcgggcaagc
     3901 cggtgttcgt ggcccattcc ttcggcggct tcccgaccat gctggcggcc agcaagtacg
     3961 gcgaccggct gggggcggtg gtgctggtcg attccatggt ccgcccgccg gaactggaat
     4021 ggcgcggtcc gcccaagcgg gtcagcccca accgggtcta cccgaccctg accgaggcgc
     4081 tcggccgctt ccgcctggcc ccgccacagg gctgcgagaa cccctacatc gccgactgga
     4141 tcgcccggaa cgcgctgaag gaagtcgagg gcggctggac ctggcggttc gaccccttca
     4201 tgtggtcgtc cttcatgatg gaggaccgcg gcccgctgct ggccaacatc acctgcccgg
     4261 tggcgatgat gtggggcgac cgctcgcgcc tggccgagcc gcatatcctc gactacatgc
     4321 gtggccttct gccgcccggc tcgccgcaga tcgccatccc cgacgccgac catcacgtga
     4381 tgatcgacca gccgctggcc ttcgtcgggg cgctgcgggg gctgctggcg gggtggcccg
     4441 gagaaagggc gtagggcgta gggcgtaggg agtagttcac ccggttcccg ccgccgccac
     4501 cgcgtctgcc ctttgctact ccctactccc tactccctac tccccacgcc ctacgcccta
     4561 ttccgcccct tgagccctcg cccctcccat agtaatcccg ccccatcaga tcgcaggaca
     4621 caccgatggc ttccgaatat caccgcggcg agatggacat ctccgagcag acggcgacct
     4681 accatgccgt gatgggcatg acgaagtggg gctccctggc cctggccgtg gccatcctca
     4741 tgctgacgct gtggttctgc acgccggcgg gcttcttcgg cgggctggtt ccgggcctga
     4801 tcgtcctggt cgccggcgtc gccctgctgc gcgaaaagaa gggcgccggg cactaaagtc
     4861 cgttcgcaat ggtgcggcgc ggcatagttt ttcgcagcgc accatcggca ggctcgacaa
     4921 cgggcaagat catcccctaa acaggctccc tagggtcgcg actcacaacg cgtccggatg
     4981 gggagttcca tgccagtcat cgccgtcacc cgcgaacgtc gagccggtga aacgcgggtc
     5041 gccgcgactc ccgagacggt gaagaagctg atcgccgccg gctggtcgct gaccgtcgag
     5101 gccggcgccg gaaccgcggc ttccttcccc gacgccgact acgccgccgc cggcgcgacc
     5161 atcgccagat cgctgaagga cgccgtcggc aaggccgatg tgctgttcaa ggtgcgcggg
     5221 cccgaggccg acgagatcgc cgccctcaag cccggcgcca tggtcgtggc cacgctcaat
     5281 ccctaccagg accgcgccac gctcgagggc ctcgcgaagg cgaaggcctc ggccttcgcc
     5341 atggagttcg tgccgcggat cacccgggcc caggtgatgg acgtgctgtc ctcccaggcc
     5401 aacctcgcgg gctaccgcgc ggtgatcgag gcctcggaag cctacggccg ggccatgccg
     5461 atgatgatga ccgccgccgg cacggtggcc gcggccaagg tgttcatcat gggcgtcggg
     5521 gtcgcgggcc tgcaggccat cgccaccgcc cgccgtctgg gcgcggtggt caccgccacc
     5581 gacgtgcgcc cggccaccaa ggaacaggtc gagtcgctcg gcgccaagtt cctcgcggtc
     5641 gaggacgagg agttcaggaa cgcccagacc gccggcggct acgccaagga gatgtccaag
     5701 gagtaccagg ccaagcaggc cgagctggtc tccagccaca tcgccaagca ggacatcgtc
     5761 atcaccaccg ccctgatccc
//
LOCUS       DAORNX010000157         5766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10290_length_5766_cov_7.321485, whole genome shotgun sequence.
ACCESSION   DAORNX010000157 DAORNX010000000
VERSION     DAORNX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10290_length_5766_cov_7.321485"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLO65_08265"
     CDS             complement(<1..621)
                     /locus_tag="PLO65_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Localization factor PodJS"
                     /protein_id="HRD28279.1"
                     /translation="MTSGAPWSVKGIDPKAREIAKDLARRSGMTLGEWLNRMIVEGEP
                     VIEAGQDRPYDAPEPVILSTPRESAAPRFEVAGHPADEVGRVAEAVERLTARIEAAEQ
                     RSTLAITGIDQSVRGALSRLELAEREQIAVAARFEGAVEDLHSQYGGIGERLRRIELE
                     STGARSAEALRAIEGMLGKVAGQLYEGEARTRETLAGVEARIAAVEA"
     gene            767..970
                     /locus_tag="PLO65_08270"
     CDS             767..970
                     /locus_tag="PLO65_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28280.1"
                     /translation="MTVTTTLAITAAFLGLTAFAGWRGARPPDLLKGPRMIPWRMVMV
                     TSAAAVLILLVHLASLFGLHHDR"
     gene            complement(977..>1838)
                     /locus_tag="PLO65_08275"
     CDS             complement(977..>1838)
                     /locus_tag="PLO65_08275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28281.1"
                     /translation="AMDGPVGRASERMELLWRFRELADAHAARPALYGIGPEDLPDAV
                     ELGFSIQKIGEAAAVPLDGFSLAGRRREVLRRNWRKAAEGGATFEVVPPEAAAGLLPE
                     LKAVSDAWLALHAGGEKSFSLGGFVPRYVAEFPVAVVRVEGRIVAFATLWLTASRKAL
                     SMDLMRYGEAAPKNVMDFLFVELFAWGRDQGFQAFDFGAAPLAGLEDRPLAPIMSRVG
                     RLLFERGEEIYNFRGVRRYKDKYDPLWQPRYIAAPHKWQIPMLLADVGLLSSGGVAGL
                     TRRPRRGGEG"
     assembly_gap    1839..1944
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1945..2624)
                     /locus_tag="PLO65_08280"
     CDS             complement(<1945..2624)
                     /locus_tag="PLO65_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin resistance protein FmtC"
                     /protein_id="HRD28282.1"
                     /translation="MRLYGELKPAILAAAPTLAAIAAAASGVMLLASGATPSDPSRLV
                     ELLKVTPTALIEISHFLSSILGLVLILLAFGLRSRLDAAWTASLITLLAAALLAPFKG
                     FNWEEAAVLIACFLVILPLHNAFPRAAGLSRMEVTPGWLASAAAVLGGAALLGWWSFA
                     HADYAQQPVWRVMADQDLTRAIRSSGGAALALLLFGVWRLFAMPATPPVAGDDDPDFD
                     RVRAILRSA"
     gene            2772..3980
                     /locus_tag="PLO65_08285"
     CDS             2772..3980
                     /locus_tag="PLO65_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28283.1"
                     /translation="MADFGGGDLEAFRAEVRDWLAANYPASLNAPMDDESEAPWGGRR
                     FKPKNPDAKLWLDRMASKGWTAPMWPKAYGGGGLTREQNKVLESELRRIRARPALMSF
                     GIWMLGPVLLEYATEEQKQKFLPGIVKGEIRWCQGYSEPGAGSDLAGLQTRCEDKGDH
                     FLINGQKVWTSYADQADWIFCLVRTDTSKKHEGISFVLFDMTSEGVETRPIKLISGSS
                     PFCETFFTDVKVPKDQLVGKLNGGWEIAKRLLQYERQNISAGGFGGGAGIDVVEAAKT
                     HLGVDAEGRIADADLRTRIAAHKMDQHAFMLTIRRAEAEAKASKGPSAATSIIKYAAA
                     KLNQERTELTVEALGLQGLGWEGDSFSAGEIGAMRSMLRAKANSIEGGTSEINLNVVA
                     KRVLGLLDHQ"
     gene            3980..4588
                     /locus_tag="PLO65_08290"
     CDS             3980..4588
                     /locus_tag="PLO65_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRD28284.1"
                     /translation="MIEFWYEFASTYSYPAAMRIEALAEAAGVQVAWRPFLLGPLLHA
                     QQGMTDSPFNVVPVKGRYMWRDMERVCEEQGLPLTHPSQFPRNTLLAARVAVAGLEDG
                     WTPAFTRRVYEANFVADQDISKPEVLAPLLEEVGASPDAVFAAAQGDGVKARLKDHVE
                     QALGRGVFGSPSFITADGELFWGNDRLEQALAWAVHHEKATA"
     gene            4719..>5766
                     /locus_tag="PLO65_08295"
     CDS             4719..>5766
                     /locus_tag="PLO65_08295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28285.1"
                     /translation="MAVLNEEQTMLRDAAKSWAQESSPVTRLRALRDGKSGRSFDPAT
                     WAEMGQMGWAGVIVPEDYDGTAFGYLSLGLILEETGRTLVASPLLSTALIGAAALTLG
                     GSEAQKTAWLPKIAAGEIVATLAVDEKAHHAPEMTALKAEKAGDGWSLTGSKTFVLDG
                     DAADLIVVAARTGGQPGETDGITLFLVEGGAAGVTRKHLALADSRGAAEITFAGAKGE
                     VLGEVGKGWAILEPVLDRARAGLAAEMLGSALQAFEITLDYLKTRTQFGQVIGTFQSL
                     QHRAAKMFTDLETTRSCVEAALEQIDAGASDNRAAASLAKAKASELAHLVSNEMVQMH
                     GGIGMTDVHDAGLYL"
BASE COUNT          927 a         2011 c         1972 g          750 t
ORIGIN      
        1 ggcctcgacc gccgcgatcc gtgcctcgac gccggccagg gtctcacggg tgcgggcctc
       61 gccctcgtag agctgcccgg cgaccttgcc gagcatgccc tcgatcgcgc gcagggcctc
      121 ggcggagcgg gcgccggtcg actccagctc gatccggcgc agccgttcgc cgatgccgcc
      181 atactggctg tgcaggtctt ccacggcgcc ttcgaaacgc gccgccacgg cgatctgttc
      241 acgttccgcc agctccagcc gcgaaagggc gccgcggacc gactgatcga tgccggtaat
      301 ggccagggtc gagcgctgct cggcggcttc gatgcgggcg gtcagccgtt cgacggcctc
      361 ggcgacgcgg ccgacctcgt cggcgggatg gcccgcgacc tcgaaccggg gcgcggccga
      421 ctcgcgtggc gtcgaaagga tgaccggttc cggcgcgtcg tagggtcggt cctgcccggc
      481 ctcgatcacc ggctcgccct cgacgatcat ccggttcagc cattcgccga gcgtcatgcc
      541 ggagcgacgg gcgagatcct tggcgatctc ccgcgccttt gggtcgatac ccttcacgct
      601 ccagggcgcc cccgacgtca ttcgaatcgt gtctccggca ctcataaagg acgctaacca
      661 gccagagatt gtgtgtaaac gcagggttaa cgctaaatct agtgggtgtg acgattagct
      721 gtggacgaag cttgcaacgg ccccatccgg ccgccatctg gcccggatga ccgtgaccac
      781 gaccctggcg atcaccgccg ccttcctggg cctgaccgcc ttcgccggat ggcggggggc
      841 gcggccgccc gacctgctca aggggccgcg gatgatcccg tggcggatgg tcatggtcac
      901 ctcggccgcc gccgtgctga tcctgctggt gcatctggcc agcctgttcg gcctgcacca
      961 cgaccggtag ggccggtcag ccctcgccgc ctcgccgggg tcgccgcgtc agtccggcca
     1021 cgccgcccga cgacagcagg ccgacatcgg ccagcagcat cggaatctgc cacttgtgcg
     1081 gggcggcgat atagcgcggc tgccagagcg ggtcgtactt gtccttgtaa cgccgcacac
     1141 cgcggaaatt gtagatttcc tcgccccgct cgaacagcag ccggccgacc cgtgacatga
     1201 tcggcgccag cggccggtcc tcgaggccgg ccagcggcgc ggccccgaag tcgaacgcct
     1261 ggaagccctg gtcccggccc caggcgaaca gctcgacgaa caggaagtcc atcacgttct
     1321 tcggcgccgc ctcgccgtag cgcatcaggt ccatcgagag ggccttgcgc gaggcggtca
     1381 gccacagggt ggcgaaggcc acgatccgcc cctcgacccg caccacggcc accggaaact
     1441 cggccacata gcgcggcacg aagccaccga gggaaaagga cttctcaccg ccggcgtgga
     1501 gcgccagcca ggcgtcggaa accgccttga gctcgggcag caggccggcc gccgcctctg
     1561 gcggaacgac ctcgaacgtg gcgccgccct cggcggcctt gcgccagttg cgccgcagca
     1621 cctcgcggcg acgcccggcc agggagaagc cgtcgagcgg cacggccgcc gcctcgccga
     1681 tcttctggat cgagaagccc agctcgacgg cgtccggcag atcctccggc ccgatgccgt
     1741 agagggccgg gcgggcggca tgggcgtcgg ccagttcgcg gaagcgccag agcagttcca
     1801 tccgctcgct cgcccggccg accgggccgt ccatggcgnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnngcggag cgcaggatgg cgcggacacg gtcgaagtcg
     1981 gggtcgtcgt cgccggccac cggcggggtc gccggcatgg cgaacaggcg ccagaccccg
     2041 aacagcagca gcgccagcgc cgcaccgccc gacgaccgga tggcgcgggt caggtcctgg
     2101 tcggccatca cccgccagac cggctgctgg gcgtagtcgg cgtgggcgaa cgaccaccag
     2161 ccgagcaggg ccgcgccgcc cagcacggcc gcggcggagg cgagccagcc cggcgtcacc
     2221 tccatccgcg acaggccggc ggcccgcgga aaggcgttgt gcagcggcag gatgaccagg
     2281 aagcaggcga tcagcaccgc cgcctcttcc cagttgaacc ccttgaacgg cgccagcagg
     2341 gccgcggcca gcagggtgat caggctggcc gtccaggccg cgtccagccg cgagcgcagg
     2401 ccgaaggcca gcaggatcag cacgaggccg agaatcgagg agaggaagtg gctgatctcg
     2461 atcagcgccg tcggcgtgac cttgagcaac tcgaccaggc gggagggatc cgaaggggtt
     2521 gctccggacg ccagcagcat cacgcccgac gccgcggcgg cgatcgccgc cagggtcggg
     2581 gcggccgcca ggatggccgg cttcagttcg ccataaagcc gcatgcgatt cccaacgcct
     2641 gtccggccac ccttatagcc tgtccggcgg cgccgcgctc ggccgatgag tcgaacgggc
     2701 gtttgaaccg gcttgctccg gcgggcgcgc acgctaagct gcgccaaacg ggaattcacg
     2761 ggagaacggc catggcggat tttggcggcg gcgaccttga ggccttccgc gcggaggtgc
     2821 gcgactggct ggcggccaac tatccggcct cgctgaacgc gccgatggac gacgagtccg
     2881 aggcgccctg gggcggccgg cggttcaagc cgaagaaccc cgacgccaag ctgtggctcg
     2941 accggatggc gtcgaagggc tggaccgccc cgatgtggcc caaggcatac ggcggcggcg
     3001 ggctgacccg cgaacagaac aaggtgctcg agagcgagct gcgccgtatc cgcgcccgcc
     3061 cggccctgat gagcttcggc atctggatgc tcggcccggt gctgctcgaa tacgccacgg
     3121 aggaacagaa gcagaagttc ctgcccggca tcgtcaaggg cgagatccgc tggtgccagg
     3181 gctacagcga gccgggcgcc ggctcggacc tggccggcct gcagacccgc tgcgaggaca
     3241 agggcgacca cttcctgatc aacggccaga aggtctggac cagctacgcc gaccaggccg
     3301 actggatctt ctgcctggtg cgcaccgaca cctccaagaa gcacgagggc atctcgttcg
     3361 tgctgttcga catgaccagc gagggcgtcg agacccggcc aatcaagctg atcagcggca
     3421 gctcgccgtt ctgcgagacc ttcttcaccg acgtgaaggt tcccaaggac cagctggtcg
     3481 gcaagctcaa cggcggctgg gagatcgcca agcgcctgct gcagtacgag cgccagaaca
     3541 tctccgccgg cggcttcggc ggcggggccg gcatcgacgt ggtcgaggcg gccaagaccc
     3601 acctgggcgt cgatgccgag ggccgaatcg ccgacgccga cctgcgcacg cggatcgccg
     3661 cccacaagat ggaccagcac gccttcatgc tgaccatccg ccgggcggaa gccgaggcca
     3721 aggcgagcaa gggccccagc gccgccacct cgatcatcaa gtacgcggcc gccaagctga
     3781 accaggagcg caccgagttg accgtcgagg cgctgggcct gcagggcctg ggctgggaag
     3841 gcgacagctt ctcggccggc gagatcggcg ccatgcgctc gatgctgcgg gccaaggcca
     3901 actccatcga gggcggcacc agcgagatca atctcaacgt cgtcgccaag cgggtgctcg
     3961 gtctgctgga tcaccagtga tgatcgagtt ctggtacgag ttcgcctcga cctactccta
     4021 tccggcggcc atgcgcatcg aggcgctggc ggaggcggca ggcgtacagg tcgcctggcg
     4081 accattcctg ctgggcccgc tgctgcacgc gcagcagggc atgaccgaca gcccgttcaa
     4141 cgtcgtgccc gtgaagggcc ggtacatgtg gcgcgacatg gagcgggttt gcgaggaaca
     4201 gggcctgccc ctgacccatc ccagccagtt cccccgaaac accctgctgg ccgcgcgcgt
     4261 ggccgtggcc gggctcgagg acggctggac gccggccttc acccgccgcg tctacgaggc
     4321 caacttcgtc gccgaccagg acatctcgaa gcccgaggtg ctggcgccgc tcctcgagga
     4381 ggtcggcgcg tcgccggatg cagtcttcgc ggccgcgcag ggcgacggcg tcaaggcgcg
     4441 cctgaaggat cacgtcgaac aggccttggg ccgcggcgtg ttcggctcgc ccagcttcat
     4501 caccgccgac ggcgagctgt tctggggcaa cgaccgcctc gaacaggccc tcgcctgggc
     4561 ggtccatcac gagaaggcca cggcatgacc gtgcgtcctt cgacacggcc ccgcgggcct
     4621 gctcaggatg acgtgcatcc gagcccccaa ccccactacg tcatgctgag cagcggccag
     4681 cggccgcgtg tcgaagcacg caccaagagg atttactgat ggccgtcctg aacgaagaac
     4741 agaccatgct gcgcgacgcg gccaagagct gggcccagga gtcctcgccg gtgacgaggc
     4801 tgcgcgcgct gcgcgacggc aagtccgggc gcagcttcga ccccgccacc tgggccgaga
     4861 tgggccagat gggctgggcc ggggtgatcg tgccggagga ctacgacggc acggccttcg
     4921 gctacctgag cctggggctg atcctcgagg agaccggccg caccctggtc gcctcgccgc
     4981 tgctctcgac cgccctgatc ggcgccgcgg ccctgaccct gggcggttcc gaggcccaga
     5041 agacggcctg gctgccgaag atcgcggccg gcgagatcgt cgccaccctg gccgtcgacg
     5101 agaaggccca ccatgcgccg gagatgaccg cgctgaaggc cgagaaggcc ggcgacggct
     5161 ggagcctgac cggcagcaag accttcgtgc tcgacggcga cgccgccgac ctgatcgtcg
     5221 tggcggcgcg cacgggcggc cagcccggcg agaccgacgg catcaccctg ttcctcgtcg
     5281 agggcggcgc ggcgggcgtg acccgcaagc atctggccct ggccgacagc cgcggcgccg
     5341 cggagatcac cttcgccggc gcgaagggcg aggtgctggg cgaggtcggc aagggctggg
     5401 cgatcctcga gccggtgctc gaccgggccc gggcgggcct ggcggccgag atgctcggca
     5461 gcgcgctcca ggccttcgag atcaccctcg actacctgaa gacccgcacc cagttcggcc
     5521 aggtgatcgg caccttccaa agcctgcagc accgggcggc gaagatgttc accgacctgg
     5581 agaccacccg ttcctgcgtc gaggcggcgc ttgagcagat cgacgccggc gcgagcgaca
     5641 accgcgcggc cgcctccctg gccaaggcca aggccagcga gctggcccat ctggtcagca
     5701 acgagatggt gcagatgcat ggtggcatcg gcatgaccga cgtccacgac gccggcctct
     5761 acctca
//
LOCUS       DAORNX010000158         5763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10301_length_5763_cov_7.521198, whole genome shotgun sequence.
ACCESSION   DAORNX010000158 DAORNX010000000
VERSION     DAORNX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10301_length_5763_cov_7.521198"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>276)
                     /locus_tag="PLO65_08300"
     CDS             complement(<1..>276)
                     /locus_tag="PLO65_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HRD28286.1"
                     /translation="VFDFLGGAVRVAGQASHGALTVDDWTTVVPADGPQGAVDVYFRP
                     ETAAINGPDGPGIRARVTGLAHRGAAVRVELDLLGEPADLLLSGDRA"
     assembly_gap    277..307
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<308..972)
                     /locus_tag="PLO65_08305"
     CDS             complement(<308..972)
                     /locus_tag="PLO65_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD28287.1"
                     /translation="MSLTLTAVTKTFGRFPALRDVSFEVRPGEFLALLGPSGSGKTTL
                     LRILAGLERPDAGAIGFAGEDLLALPARERRVGMVFQHYALFRHMTVADNIAFGLSVR
                     PRATRPAAHEIARRVSDLLRLVQLEGLERRYPSQLSGGQRQRVALARALAIEPRLLLL
                     DEPFGALDARVRRDLRRWLRRVHDETGVTTLFVTHDQEEALDLADRVAILRSGALQEV
                     GSV"
     gene            complement(1038..1760)
                     /gene="phoU"
                     /locus_tag="PLO65_08310"
     CDS             complement(1038..1760)
                     /gene="phoU"
                     /locus_tag="PLO65_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRD28288.1"
                     /translation="MTEHTVKAYEGELTALETGVAEMSAQTLEQVRAAVAAITAHDSA
                     LARSTVDGDAHLDLMEARIEQQAVRLIALRQPMARDLRDILGAMKIAANLERCGDLAK
                     NIAKRALAVDGADFPDPSSGSIARMGRLVADRLGQVLAAQRTRDGSLALAVWRQDAEV
                     DQQYESLFRELLTYMMADPRMISPCAHLLFVAKNLERIGDHATNIAEAIHYQITGDPA
                     PGAGRPKWDLLVQGEARETGAP"
     gene            complement(1789..2595)
                     /gene="pstB"
                     /locus_tag="PLO65_08315"
     CDS             complement(1789..2595)
                     /gene="pstB"
                     /locus_tag="PLO65_08315"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRD28289.1"
                     /translation="MTDPNALDDDRHMVEPDPASVILRARDLNFFYGRTQALFDVDLP
                     VVRNQVTALIGPSGCGKSTLLRTFNRMYDLYPGQKASGEVTLDGEDLLALRDIARLRS
                     RVGMVFQKPTPFPMSIYDNVAFGVRLYRSAGRRELDAIVEQALRRAALWDEVKDKLKM
                     SGLSLSGGQQQRLCVARGIAVEPEILLLDEPASALDPISTAKLEETIDQLKKDFTIVI
                     VTHNLAQAARISDNTGFMYLGRMIEFGPTGDLFMKPSTRRAQDYVQGRFG"
     gene            complement(2592..3440)
                     /gene="pstA"
                     /locus_tag="PLO65_08320"
     CDS             complement(2592..3440)
                     /gene="pstA"
                     /locus_tag="PLO65_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRD28290.1"
                     /translation="MPLDRRLRRNLANGLFVAACGTATLVALLALGMIMASLAHQGLA
                     GLSVDIFTRSTPAPGSQGGLLNAITGSLMMCGLAMAIAVAVGLLAGTWLAEFGGKSAF
                     AHTVRFLNDVLLSAPSILIGLFVYELVVRPLHGFSGFAGAVALALIAAPVVTRTTEDI
                     LRLQPSALREAGAALGAARWITIRKIIWPGAGAGLLTGALLAFARISGETAPLLFTAL
                     NNQFLNLDMGKPMASLPAVIFQFALSAYDDWRSLAWTGALLIALAVLFVNIIGRVIAA
                     RGIYRP"
     gene            complement(3444..4394)
                     /gene="pstC"
                     /locus_tag="PLO65_08325"
     CDS             complement(3444..4394)
                     /gene="pstC"
                     /locus_tag="PLO65_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRD28291.1"
                     /translation="MTDLAATDRAAPRGAWFDPIFRFACLSAATLLLAALGGVVISLA
                     IGGWPALSRFGVDFFLTTTWNPVTEVYGAAGPIVGTLATSALSLAMALPVALGVAVFL
                     VEFCPRLLAQPIAIAVELLAGIPSIVYGMWGLFVLAPFFALHVQIPIIMTLPDDSFWG
                     KLLAGVPNGANIFTASLILAIMILPYMAAVFRELLLTVPAQVREAAYGVGCTPLEVVT
                     AVSFPYVRTGAVGVIMLGLGRALGETMAVTFIIGNSHDLPTSLFGSGSTIASTIANEF
                     TEATGEMHTAALTALGLVLFVITFAVLAIARALLIAQRED"
     gene            complement(4462..5502)
                     /gene="pstS"
                     /locus_tag="PLO65_08330"
     CDS             complement(4462..5502)
                     /gene="pstS"
                     /locus_tag="PLO65_08330"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRD28292.1"
                     /translation="MFKTIAVALGAALLAGTAAMAGDISGAGATFPAPVYAKWAEAYK
                     TATGIGLNYQAIGSGGGIKQIKARTVAFGASDKPLTPAELNAAGLMQFPTVMGGVVPV
                     VNLPGVKPGQLHLTGAVLADIYRGAITKWNAPQIAALNRGVKLPALPITVVHRSDGSG
                     TTFLFTSYLSMKSPAWKTSVGASDSVQWPTGLGGKGNDGVAAFVKQTIGSIGYVEYAY
                     AKQNGATFVLVQNRAGQYPAPVAANFAAAAAGADWTKAPGNYLLLLDEPGAKSWPITG
                     ATFILVYKKQTDAASGAAVLRFFDWAYKNGDAAAGALDYVPMPASVKALMRKQWAAQI
                     TGPDGKPVYVSR"
BASE COUNT          905 a         1902 c         2116 g          809 t
ORIGIN      
        1 tcgcccggtc gccggacagc agcaggtcgg ccggttcgcc cagcaggtcc agctccaccc
       61 gcacggcggc gccccggtgc gccaggcccg tgacccgcgc gcggattccc gggccgtcgg
      121 gtccattgat ggcggcggtc tccggccgga agtagacgtc gaccgccccc tgggggccgt
      181 cggcgggcac gacggtggtc cagtcgtcga cggtcagggc gccgtgactg gcctggccgg
      241 ccacccgcac cgcgccgccg aggaagtcga agacgannnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnntca acgcttccaa cttcctggag cgcgccgctg cgcaggatgg cgacccggtc
      361 ggcgaggtcg agcgcctctt cctggtcgtg ggtcacgaag agggtcgtca cgcccgtctc
      421 gtcgtgcacg cgacgcagcc agcggcgcag gtcacggcga acccgggcgt caagcgcgcc
      481 gaagggctcg tcgagcagca gcaggcgcgg ctcgatcgcc agggccctgg ccagggccac
      541 gcgctggcgc tgaccgccgg acagctgcga gggataacgg cgctccaggc cttccaactg
      601 gaccaaccgc agcaggtcgc tgacccgccg cgcgatctca tgcgcggccg ggcgggtggc
      661 gcgcggccgc acgctgaggc cgaaggcgat gttgtcggcc acggtcatgt ggcgaaacag
      721 ggcatagtgc tgaaacacca tgccgacccg ccgctcgcgc gcgggcaggg ccagcaggtc
      781 ctcgcccgcg aaaccgatgg ccccggcgtc gggccgctca agcccggcga ggatgcgcag
      841 cagcgtggtc ttgcccgagc cggacggacc gagcagggcg aggaactccc ccggccgcac
      901 ctcgaacgac acgtcccgca gggcgggaaa tcggccgaac gtcttggtta cggcggtgag
      961 ggtcagcgac atcggagctc gggcggtcgg cggcgggccg ggaacgcctc attcgagccg
     1021 ggatttcccc gccggcgtca aggggcgccg gtctccctgg cctcgccctg gaccagcagg
     1081 tcccacttcg gccgtccggc gcccggcgcc ggatcgccgg tgatctgata gtggatcgcc
     1141 tcggcgatgt tggtcgcgtg gtcgccgatg cgctcgaggt tcttggccac gaacagcagg
     1201 tgggcgcagg gcgagatcat ccgcgggtcg gccatcatgt aggtgagcag ttcgcggaac
     1261 aggctctcgt actgctggtc gacctcggcg tcctggcgcc agacggcgag ggcgagcgac
     1321 ccgtcgcgcg tgcgctgcgc ggcgagcacc tggcccaggc ggtcggcgac cagtcgaccc
     1381 atgcgggcga tcgacccgct gctcgggtcg ggaaagtcag cgccgtcgac cgccagcgcc
     1441 cgcttggcga tgttcttggc aaggtcgccg cagcgctcca ggttggcggc gatcttcatc
     1501 gccccgagaa tgtcgcgcag atcccgcgcc atcggctggc gcagggcgat cagccgcacc
     1561 gcctgctgtt cgatccgggc ctccatcagg tcgaggtggg cgtcgccgtc gaccgtcgag
     1621 cgggccagcg cggaatcatg ggcggtgatc gccgcgacag cggcccgcac ctgctcgagc
     1681 gtctgggcgc tcatctcggc gacgccggtc tccagcgccg tcagttcgcc ttcgtaggcc
     1741 ttgaccgtat gttccgtcat gccggttcac tccgctggga tggccgcgtc agccgaaacg
     1801 gccctggacg tagtcctggg cgcggcgggt cgacggcttc atgaacaggt cgccggtcgg
     1861 cccgaactcg atcatccggc ccaggtacat gaagccggtg ttgtccgaga tgcgggcggc
     1921 ctgggcgagg ttgtgggtca cgatgacgat cgtgaagtcc ttcttcagct gatcgatcgt
     1981 ttcctcgagc ttggccgtgg agatcggatc gagcgccgag gcgggctcgt cgagcagcag
     2041 gatctcgggc tcgacggcga tgccgcgggc cacgcagagc cgctgctgct ggccgcccga
     2101 caggctgagg ccggacattt tcagcttgtc cttgacctcg tcccacaggg cggcccggcg
     2161 cagggcctgt tcgacgatgg cgtcgagctc gcgacggccg gcgctgcggt agagccggac
     2221 gccgaaggcg acattgtcat agatcgacat cgggaagggc gtcggcttct ggaacaccat
     2281 gccgacccgc gaccgcagcc gcgcgatgtc gcgcagggcc agcaggtcct ccccgtcgag
     2341 cgtcacctcg cccgaggcct tctggccggg atagaggtcg tacatccggt tgaaggtccg
     2401 cagcagggtc gacttgccgc agcccgacgg gccgatcagc gcggtgacct ggttgcgaac
     2461 gaccggcagg tcgacgtcga acagcgcctg ggtccggccg tagaagaagt tcaggtcgcg
     2521 ggcgcgcagg atcacgctgg ccgggtccgg ctcgaccatg tggcgatcgt cgtcgagggc
     2581 gttggggtcg gtcatgggcg atagattccg cgggcggcta tgacccgccc gatgatgttc
     2641 acgaagagga cggccagcgc gatgagcagg gcgccggtcc aggccaggct gcgccagtcg
     2701 tcgtaggcgc tgagggcgaa ctggaagatc accgccggca ggctggccat cggcttgccc
     2761 atgtccaggt tgaggaactg gttgttcagg gcggtgaaca gcagcggcgc ggtttcgccg
     2821 ctgatgcgcg cgaaggccag cagggcgccg gtcagcaggc cggctccggc gccgggccag
     2881 atgatcttgc ggatggtgat ccaccgggcc gcgccaaggg cggcgccggc ctcgcgcagg
     2941 gccgagggct gcaggcgcag gatgtcctcg gtggtgcggg tcaccaccgg ggcggcgatc
     3001 agcgccaggg ccacggcgcc ggcgaaaccg gaaaagccgt gcagcggccg caccaccagc
     3061 tcgtagacga acaggccgat gaggatcgac ggcgccgaca gcagcacgtc gttgaggaac
     3121 cgcacggtgt gggcgaaggc cgacttgccg ccgaactcgg ccagccaggt tccggccagc
     3181 aggcccacgg ccacggcgat cgccatcgcc agcccgcaca tcatcagcga gcccgttatg
     3241 gcgttgagca gcccgccttg cgaccctggc gcgggggtcg agcgggtgaa gatgtcgacg
     3301 ctcaggccgg ccaggccctg atgcgccagc gaggccatga tcatgcccag cgcgagcagg
     3361 gcgacgaggg tcgccgtccc gcaggcggcg acgaacaggc cgttggcgag gttgcgacga
     3421 aggcggcggt ccagcggcat ggctcagtcc tcccgctggg cgataagcag cgcgcgggcg
     3481 atggccagca cggcgaaagt gatgacgaac agcaccaggc ccagggccgt cagcgcggcc
     3541 gtgtgcatct cgcccgtggc ctcggtgaac tcgttggcga tggtcgaggc gatggtcgag
     3601 ccgctgccga acagcgaggt cggcaggtca tgcgagttgc cgatgatgaa ggtcacggcc
     3661 atggtctcgc cgagcgcgcg gccaaggccc agcatgatca cccccaccgc gccggtgcgc
     3721 acgtagggaa agctgaccgc cgtgacgacc tccagcggcg tgcagccgac gccgtaggcg
     3781 gcttcgcgca cctgggcggg gaccgtcagc aacagctcac ggaacaccgc cgccatatag
     3841 ggcaggatca tgatcgccag gatcagcgac gcggtgaaga tgttggcccc gttggggacg
     3901 ccggccagga gtttgcccca gaacgagtcg tccggcagcg tcatgatgat cgggatctgc
     3961 acatgcaggg cgaagaacgg cgccagcacg aacagccccc acatgccgta gacgatcgac
     4021 ggaatgccgg cgagcagctc gacggcgatg gcgatcggct gggccagcag ccgggggcag
     4081 aactccacca ggaagaccgc cacgcccagg gcgacgggca gggccatggc cagcgacagg
     4141 gccgacgtcg ccagggtccc gacgatcggg ccggcggcgc cgtagacttc ggtcaccggg
     4201 ttccaggtgg tggtcaggaa gaagtcgacg ccgaacctgc tcagcgccgg ccagccgccg
     4261 atcgccagcg aaatcaccac cccgccgagg gccgccagca acagcgtcgc ggccgacagg
     4321 caggcgaagc ggaagatcgg gtcgaaccag gcccctctgg gcgcggcgcg gtcggtggct
     4381 gcaaggtcgg tcatcatgtc cggtcgcgaa aaggggcgga cgtccgaaga cgtccgccca
     4441 ccgcttcgtc ggggaggtcg atcagcggga aacgtagacc ggcttgccgt ccggcccggt
     4501 gatctgggcg gcccactgct tgcgcatcag ggccttcacg ctggcgggca tcggcacgta
     4561 gtcgagcgcg ccggcggccg cgtcgccgtt cttgtaggcc cagtcgaaga accgcagcac
     4621 cgcggcgccg gacgcggcgt cggtctgctt cttgtagacc aggatgaagg tggcgccggt
     4681 gatcggccag gatttggcgc ccggctcatc gagcagcagc aggtagttgc ccggggcctt
     4741 ggtccagtcg gcgccggcgg cggcggcggc gaagttggcc gcgaccgggg cgggatactg
     4801 gccggcccgg ttctggacca ggacgaacgt ggcgccgttc tgcttggcgt aggcgtattc
     4861 gacgtagccg atcgagccga tcgtctgctt gacgaaggcg gccacgccat cgttgccctt
     4921 gccgcccagt ccggtcggcc actgcaccga gtcgctggcg ccgacgctcg tcttccaggc
     4981 ggggctcttc atcgacaggt aggaggtgaa caggaaggtc gtgcccgagc cgtccgaacg
     5041 gtgaacgacg gtgatcggca gggcgggcag cttgacgccc ctgttgaggg cggcgatctg
     5101 cggggcgttc cacttggtga tggcgccgcg atagatgtcg gcgagcaccg cacccgtcag
     5161 gtgcagctgg cccggcttca cgcccggcag gttcaccacc ggaacgacgc cgcccatgac
     5221 ggtcggaaac tgcatcaggc cggccgcgtt gagctcggcg ggcgtgagcg gcttgtccga
     5281 ggcgccgaac gcgacggtcc gggccttgat ctgcttgatg ccgccgcccg aaccgatcgc
     5341 ctggtagttc aggccgatgc cggtcgcggt cttgtaggcc tcggcccact ttgcatagac
     5401 cggagcgggg aaggtggcgc cggcgccgga aatgtcgccg gccatggcgg cggtgcccgc
     5461 cagaagggcc gcgccgagcg cgaccgcgat tgtcttgaac atccgtcttc tccacgagcc
     5521 gcatgccgtc atgcggcctt cgcggagagg gcctagccac acaatgtaac gtgcatgtga
     5581 cgcactgtaa gacgagctta ctttgtggtc agactttttt gcatccgggc ctgctgcagg
     5641 gccaggctgc ggctggcttc gaggcgaagc cacatggcga accgggccgc cagatccgcc
     5701 tggcccgggc cggcgctcag cagcagatga cggccggtcg gaaccgcgaa gggcagggcg
     5761 acg
//
LOCUS       DAORNX010000159         5760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10309_length_5760_cov_6.807713, whole genome shotgun sequence.
ACCESSION   DAORNX010000159 DAORNX010000000
VERSION     DAORNX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10309_length_5760_cov_6.807713"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLO65_08335"
     CDS             complement(<1..738)
                     /locus_tag="PLO65_08335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRD28293.1"
                     /translation="MRLNAQDRRVWDAAATLEALGGAHVALWVWEPEEDRFRLYGAAR
                     QLGLGPLSPECSSAAFRALALPQDRAGAEELLRIQSPGSEIALRIRMRGGETCLWRGV
                     WLEEGVRASGVVAPETRFAASEFDSLTGLLDRPSFIARARERLTQPSRQTLVVADLDR
                     LRRLNEALGHDRADLVLAALGSRLAAAFGSGAIMARVGEDEFAVMTSGDPEPTERLRK
                     ALEQPLRIAGFDILPTLAIGAVEAEGGE"
     gene            complement(762..1355)
                     /locus_tag="PLO65_08340"
     CDS             complement(762..1355)
                     /locus_tag="PLO65_08340"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD28294.1"
                     /translation="MSLLEWISPEPGPALQGEGVRLRAPHRRDYAEWAALRQASRAFL
                     QPWEPVWSPDELSLAAYSRRLAAWRNGRDLDQMYAFFIFRETDEALVGGITLNNVRRG
                     VAQMGTVGYWSGQAFARQGHTLAALRVLIGFAFGPLALHRLEAACLPTNDASASLLLK
                     AGFTEEGYAKSYLKINGDWRDHRLFGLVPREGARGRP"
     gene            complement(1423..2688)
                     /locus_tag="PLO65_08345"
     CDS             complement(1423..2688)
                     /locus_tag="PLO65_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD28295.1"
                     /translation="MTGRLHTLPNGVRVVFDPMPGLETLALTVVAGRGARYEDETRSG
                     WSHLLEHMVFKGAGGRTARAIVEDVEAAGGHLNAATGYERTSFQTRTLAGGLPLAMRV
                     TADLVLRPTLDPGDLAREKQVVGQEIAEALDTPDDLVFELAQEQAFAGQSLGRPILGT
                     MKSLKAATPQALSDWRAALYAPDRLVVSVAGKVDEDDLLGLAEEAFGHLPDHGGAEPP
                     PAPAFTGGRAARLRKLEQAHVVLLLPAVGASDPDYFVLRLFAEALGGGMSSRLFQEAR
                     EKRGLAYAIDAYADTYVDAGMLGVYAGCAAANAAELARIAAGEIQALAASVGETELTR
                     AKAQLKASLFMGREHPLNRAEQAAGQTLMFGHLYTPAAISQAIDQVTAADFARLGERL
                     VADRRCVVSVLGSKTALAAPDAFERAVFG"
     gene            complement(2685..4094)
                     /gene="thrC"
                     /locus_tag="PLO65_08350"
     CDS             complement(2685..4094)
                     /gene="thrC"
                     /locus_tag="PLO65_08350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRD28296.1"
                     /translation="MKYVSTRTGSGPDAPAVGFLEAILSGLAPDGGLYVPETWPELTA
                     DEIAAFAGQPYHRVAAEILGRFTGGEIPHDALDEMCEEAYATFTHAATCPVKQLTPGM
                     FLVELFHGPSLAFKDVAMQLLGRLYDYALARHGRTMTVVCATSGDTGGAAVEAFRGRA
                     NARIVALFPEGRISEVQRRFMTTTGEANVACVSVDGTFDDCQAILKTMFGDDQFRSAV
                     DLSGVNSINFARIAAQAVYYFTAAVALGAPHRGVAFTVPTGNFGDAFAGWVARRMGLP
                     ITGILVATNSNDIVSRAFETGRYARGGVTPTQSPAMDIQVASNFERLYFESVRRDGVE
                     TGRAFRAFAETGVIDLPPQALSAMRETFTGAAVSEDDTARCMLSTLNETGELVDPHTA
                     VALAAARRASLPDPTTPMAILSTAHPAKFPEAVSAATGTTPDVPAAARGLADRAERYD
                     HLPADADAIMAYVRAFVTR"
     gene            complement(4091..4792)
                     /locus_tag="PLO65_08355"
     CDS             complement(4091..4792)
                     /locus_tag="PLO65_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HRD28297.1"
                     /translation="MTARRFPIGLTLATAVALAILCGLGVWQLKRLAWKTELLASIAA
                     AQAAPPRPIGQTPLTPAYRFRRVTLDCPGLAGAPFVELYAINSDGVAGRRLVSLCRPV
                     GARTQILVDRGFVADTVSARPPVQPSTAVVHLTGVLREGETPGAFTPPTRDGLFYVRD
                     IGQMARALGATRHPRVMILAETSSNPAWKALVPTPLPPDIPNRHLEYALTWFGLAGAL
                     VAVYAALLRRGLKAA"
     gene            complement(4782..5138)
                     /locus_tag="PLO65_08360"
     CDS             complement(4782..5138)
                     /locus_tag="PLO65_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRD28298.1"
                     /translation="MVNPYAAGAAGRCPNCGEGHLFEGFLRIAPTCEACGFDLKTADT
                     GDGPAVFVILIGGFLVAFGAVFTEIAFSPPIWLQLLVWLPLTAVVCGALLRPFKGLLI
                     AAQFTNKAREAGRDDR"
     gene            complement(5140..>5760)
                     /locus_tag="PLO65_08365"
     CDS             complement(5140..>5760)
                     /locus_tag="PLO65_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRD28299.1"
                     /translation="RYGMILFIASEVMFFVAWFWIFFEMVLFQGHRTLTPEIGNFEGV
                     AEAWKTWPPKGVELVPAFHLPLVNTLTLLLSGTTVTWAHHALQQGDRKAAKIGLFLTI
                     ALGVLFTSIQAYEYNHILHEKLFYSEEAANAGLYGSSFFMATGFHGFHVLIGTIFLTV
                     CFFRLLGGGFTPKQHFGFEAAAWYWHFVDVVWLFLFAFIYVIFGTA"
BASE COUNT          908 a         1943 c         2145 g          764 t
ORIGIN      
        1 ctccccgccc tcggcctcca cggcgccgat cgccagggtc ggcaggatgt cgaagccggc
       61 gatacgcagc ggctgttcga gggccttgcg caggcgctcg gtgggctcgg gatcgcccga
      121 ggtcatcacc gcgaactcat cctcgccgac ccgggccatg atggctcccg agccgaaggc
      181 ggcggccagg cgcgaaccca gcgcggccag caccaggtcg gcgcggtcgt ggccgagggc
      241 ctcgttgagt cgccgcagcc ggtcgagatc ggcgacgacc agcgtctggc ggctgggctg
      301 ggtcagccgc tcgcgggcgc gggcgatgaa actggggcgg tcgagcagac cggtcaggct
      361 gtcgaattcc gatgccgcga agcgggtctc gggggccacc acgcccgatg cgcggacgcc
      421 ttcctccagc cagaccccgc gccacaggca ggtctcaccg ccccgcatgc ggatgcgcag
      481 cgcgatttcc gatcccgggc tctggatgcg caggagctcc tcggcgccgg cgcgatcctg
      541 gggcagggcc agggcgcgga aagcggccga tgaacattcc ggggagagcg gccccaggcc
      601 gagttgccgc gcggcgccgt agaggcggaa gcggtcctcc tcgggctccc agacccaaag
      661 ggccacatgc gcgccgccga gcgcctccag cgtggcggcc gcgtcccaca ctcgtcgatc
      721 ctgggcgttg agcctcatga ccggttcacg ccggcttctc cttacgggcg cccccgagcg
      781 ccttcgcgcg ggaccaaccc gaacagccga tgatctcgcc aatcgccgtt aattttcaga
      841 taagacttgg cgtagccttc ctcggtgaag ccggccttca gcagcaggct ggcggaggcg
      901 tcgttggtcg gcaggcaggc ggcctccagg cggtgcaggg cgagcgggcc gaaggcaaac
      961 ccgatcagca ccctgagggc ggccagggtg tgcccctggc gggcgaaggc ctggcccgac
     1021 cagtagccga ccgtgcccat ctgggcgacg ccgcgacgga cgttgttgag ggtgatgccg
     1081 ccgaccagcg cctcatcggt ctcgcggaag atgaagaagg cgtacatctg gtccaggtcc
     1141 cgaccgttgc gccaggccgc cagccgtcgg gagtaggcgg ccaggctcag ctcgtctggc
     1201 gaccagaccg gctcccaggg ctgcaggaag gcgcgcgaag cctgccgcag cgccgcccat
     1261 tcggcgtagt cccggcggtg gggcgcgcgc aggcgcacac cctctccctg gagggcgggg
     1321 ccgggttcgg gcgagatcca ctcgaggagt gacatgggcg aagcgtggcg cgcatcgagg
     1381 acgcgggcaa gaggcgcctg ccgcctctcc gtggaggaag gtctagccga acaccgcccg
     1441 ttcgaacgcg tccggcgccg ccagcgccgt cttcgagccc agcaccgaca cgacacagcg
     1501 cctgtccgcc accagccgct cgccgagccg cgcgaagtcg gccgcggtca cctggtcgat
     1561 ggcctgggag atggcggccg gcgtgtagag gtgaccgaac atcagggtct ggcccgcggc
     1621 ctgttcggcg cggttgaggg ggtgttcgcg gcccatgaac aggctggcct tgagctgggc
     1681 cttggcccgc gtcagctcgg tctcgcccac gctggccgcc agcgcctgga tctcgccggc
     1741 ggcgatgcgc gccagttcgg cggcgttggc cgccgcgcaa ccggcgtaga cgccgagcat
     1801 gccggcgtcg acgtaggtgt cggcataggc gtcgatggcg taggccagcc cgcgcttctc
     1861 gcgcgcttcc tggaacagcc gcgaggacat gccgccgccg agggcctcgg cgaacagccg
     1921 caggacgaag tagtcgggat cagaggcgcc gacggcgggc agcaacagca ccacatgggc
     1981 ctgctccagc ttgcgcagcc tggccgcgcg gccgccggtg aaggccggcg cgggcggcgg
     2041 ctcggccccg ccatggtcgg gcaggtggcc aaaggcctct tccgccagac cgagcaggtc
     2101 atcctcgtcg acctttccgg cgacgctgac caccagccgg tcaggagcgt atagggcggc
     2161 gcgccagtcg gacagggcct gcggcgtggc ggccttcagc gacttcatcg tgcccaggat
     2221 cggccggccc agcgactggc ccgcgaacgc ctgttcctgg gccagttcga acaccaggtc
     2281 gtcgggcgtg tcgagcgcct cggcgatctc ctggccgacc acctgcttct cgcgcgccag
     2341 gtccccggga tcgagggtcg ggcgcagcac cagatcggcg gtgacgcgca tggccagcgg
     2401 caggccgccg gccagggtgc gggtctggaa gctggtccgc tcgtagccgg tggccgcatt
     2461 gagatggccg ccggccgcct cgacgtcctc gacgatggcc cgcgcggtcc ggccgccggc
     2521 ccccttgaac accatgtgct cgagcaggtg agaccagccg ctgcgggtct cgtcctcgta
     2581 gcgggcgccg cggccggcca ccacggtcag ggccagggtc tcgagcccgg gcatcgggtc
     2641 gaacaccacc cgaacgccgt tgggcagggt gtggaggcga cccgtcaccg ggtcacgaac
     2701 gcgcgcacat aggccatgat ggcgtccgcg tccgccggca gatggtcgta gcgctcggcc
     2761 cggtcggcca ggccgcgcgc ggcggccgga acatcgggcg tggtcccggt ggccgcgctg
     2821 acggcctcgg ggaacttggc cggatgggcc gtcgacagga tggccatcgg ggtcgtcgga
     2881 tcgggcagcg aggcgcgccg ggccgccgcc agggccacgg cggtgtgcgg atcgaccagt
     2941 tcgccggttt cgttcagcgt cgacagcatg cagcgcgcgg tatcgtcctc gctgaccgcc
     3001 gcgccggtga aggtctcgcg catggccgac agggcctggg gcggcaggtc gatgacgccg
     3061 gtctcggcga aggcgcggaa ggcgcggccg gtctcgaccc cgtcgcggcg gacgctttcg
     3121 aaatagaggc gctcgaaatt cgaggcgacc tggatgtcca tggccggcga ctgggtcggc
     3181 gtcaccccgc cgcgggcgta gcgaccggtc tcgaaggcgc gggagacgat gtcgttgctg
     3241 ttggtcgcca cgaggatgcc agtgatcggc aggcccatgc ggcgagccac ccagccggcg
     3301 aaggcgtcgc cgaagttgcc ggtcggcacg gtgaaggcca cgccccggtg cggcgcgccg
     3361 agcgccactg cggcggtgaa atagtagacg gcctgggcgg cgatgcgggc gaagttgatc
     3421 gagttgacgc ccgacaggtc cacggcgctg cggaactggt cgtcgccgaa catggtcttg
     3481 aggatcgcct ggcagtcgtc gaaggtgccg tcgaccgaga cacaggcgac gttggcctcg
     3541 ccggtcgtgg tcatgaagcg gcgctgaacc tcgctgatgc ggccctcggg aaacagcgcg
     3601 acgatgcggg cgttggcccg gccccggaac gcctcgaccg ccgccccgcc cgtgtcgccg
     3661 gacgtggcgc agaccacggt catggtccgg ccgtgacggg ccagggcgta gtcgtacagc
     3721 cgcccgagca gctgcatcgc cacgtccttg aaggccaggg acggaccgtg gaacagctcg
     3781 accaggaaca tcccgggcgt cagctgcttg accgggcagg tcgccgcgtg ggtgaaggtc
     3841 gcgtaggcct cctcgcacat ctcgtcgagc gcatcgtgcg ggatctcacc gcccgtgaag
     3901 cgacccagga tctccgcggc cacgcggtgg tagggctggc cggcgaaggc ggcgatctcg
     3961 tcggcggtca gctcgggcca ggtctcgggc acatacaggc ctccgtcggg ggccaggccc
     4021 gacaggatcg cctcgagaaa gccgacagcc ggtgcgtcgg ggccggagcc cgtccgtgtg
     4081 gaaacgtatt tcatgccgct ttcaatccgc gccggaggag cgccgcatag acggcgacca
     4141 gagcgcccgc aagaccgaac caggtcaggg cgtactccag gtgccggttg ggaatgtcgg
     4201 ggggcagggg cgtcggaacc agggctttcc aggccgggtt gctcgacgtc tcggccagga
     4261 tcatcacccg ggggtggcgg gtggcgccca gggcgcgggc catctggccg atgtcgcgca
     4321 cgtagaacag gccatcgcgc gtcggcgggg tgaaggcgcc gggggtctcg ccctcgcgaa
     4381 ggacgccggt cagatggacg acggcggtcg acggctggac cggggggcgg gcggagactg
     4441 tgtccgcgac gaatccgcga tcgacgagga tctgcgtccg cgcgccgacc ggacgacaga
     4501 gcgacaccag ccgccggccg gccacgccgt cgctgttgat ggcgtagagc tccacgaatg
     4561 gcgcgccggc caggcccgga cagtcaagcg tgacccgccg gaagcggtag gccggcgtca
     4621 ggggcgtctg gccgatcggc cggggaggcg cggcctgggc cgcggcgatg ctggccagca
     4681 gctcggtctt ccaggcgagg cgcttgagtt gccacacgcc cagcccgcag agaatcgcca
     4741 aggcgaccgc ggtggcgagg gtcaggccga tggggaagcg cctagcggtc atcccgcccg
     4801 gcctcccggg ccttgttggt gaactgggcg gcgatcagca gccccttgaa cggccgcagc
     4861 agcgcgccgc agaccaccgc ggtcagcggc agccagacca gcagctggag ccagatcggc
     4921 ggactgaagg cgatctcggt gaacaccgcg ccgaaggcga ccaggaagcc gccgatcaga
     4981 atcacgaaga cggccggacc gtctccggtg tcggccgtct tcagatcgaa gccgcaggct
     5041 tcgcaggtcg gggcgatccg gagaaagccc tcgaacagat gaccttcgcc gcagttgggg
     5101 cagcgacccg ccgcccccgc cgcgtacgga ttgaccatgt caggcggtcc cgaagatcac
     5161 gtagatgaag gcgaacagga acagccagac cacgtcgacg aagtgccagt accaggcggc
     5221 ggcctcgaag ccgaagtgct gcttgggggt gaacccgccg ccgagcaggc ggaagaagca
     5281 gaccgtcagg aagatggtcc cgatcagcac gtggaagccg tggaagccgg tcgccatgaa
     5341 gaaggacgaa ccgtacaggc cggcgttggc cgcctcctcc gaatagaaca gcttctcgtg
     5401 caggatgtgg ttgtactcgt aggcctggat cgaggtgaac agcacgccca gcgcgatggt
     5461 caggaacagg ccgatcttgg ccgccttgcg atcgccctgc tgcagcgcgt ggtgcgccca
     5521 ggtcaccgtg gtgcccgaca gcagcagggt cagggtgttg accagcggca ggtggaaggc
     5581 cgggaccagc tcgacgccct tgggcggcca ggtcttccag gcttcggcca cgccctcgaa
     5641 gttgccgatc tccggcgtca gggtgcggtg gccctggaac aggaccatct cgaagaagat
     5701 ccagaaccag gcgacgaaga acatcacttc cgaggcgatg aacaggatca tgccgtagcg
//
LOCUS       DAORNX010000160         5749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10331_length_5749_cov_7.131893, whole genome shotgun sequence.
ACCESSION   DAORNX010000160 DAORNX010000000
VERSION     DAORNX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_5749_cov_7.131893"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLO65_08370"
     CDS             <1..639
                     /locus_tag="PLO65_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28300.1"
                     /translation="GGGKPVKVDIRVIATSNRDLAQAVREGTFREDLLYRLNVVNLRL
                     PPLRERPGDIVALCDHFVKKYAAANGVPERPLSAEARRRLVSHRWPGNVRELENAMHR
                     AVLLSTGAEIEEFAVRLPDGQPMSPAPDAAMARNASMAADAVSRAFVGQTVAQVEQQL
                     ILDTLGHCLGNRTHAATILGISIRTLRNKLKEYSEAGVSVPAPQAGLGANAA"
     gene            733..2760
                     /gene="flhA"
                     /locus_tag="PLO65_08375"
     CDS             733..2760
                     /gene="flhA"
                     /locus_tag="PLO65_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRD28301.1"
                     /translation="MVRGEVGLAVGVIGIIILLILPIPAFMLDVLLSLSITSAVLILM
                     TALLIKKPLEFTAFPTVLLVTTLFRLGLNLASTRLILTHGHEGAHGAGQIIKAFGELM
                     MGGNYIIGVIVFAILVIVNFVVVTKGSGRIAEVAARFTLDSMPGKQMAIDADLSSGLI
                     DQDEAKKRRKDLEMESTFFGAMDGASKFVRGDAVAGLIIVAINIVGGFLIGVIQHKIP
                     AGQAATTYTLMTIGDGLVSQVPALIISIAAGFLVSKAGVEGSANKALVAQLAMNPISL
                     GMVSASAGVIALIPAMPKIPFLVLAIAAGVLAWRRTVKENEPEVVIETAPPAEQEEEP
                     ISHALAIDDVKIELGYGLLALINDLDGRKLTDQIRALRKTLAADFGFVMPPVRILDNM
                     RLATQGYAIRIKEMECGTGEVRIGSLMAMDPRGGQVDLPGDHVREPAFGLPATWIDES
                     LKEEATFRGYTIVDPATVLTTHLTEILKDNMADLLSYAEVQKLLKDLGPEQKKLADDV
                     VPAIVTGTSLQRVLQALLRERVSIRDLPTILEGVAEAAPHTASITQLVEAVRARLGRQ
                     LCWANRGDDGALPIVTLSPDWEVAFSEALVGQGEDKQLAMAPSKLQEFIRGVREAFER
                     AALAGDSPVLLTSPQIRPFVRSIIERFRGQTVVMSQNEVHPRARLKTVGQV"
     gene            2973..3599
                     /locus_tag="PLO65_08380"
     CDS             2973..3599
                     /locus_tag="PLO65_08380"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28302.1"
                     /translation="MEPTRERILFAAMALFSEKGFLSTSVADILREAGANSGSLYHFF
                     PTKQDVLLAVLEMYRDGIYPMLLAPAWEGVDDPIERVFALLAAYRGMLKNTACLYGCP
                     IGSIALELHEPDPPVREMLSVNFEGWVRHVKGCLDAAKARLPAGTDTHALATFVLTTM
                     EGGVMQSRTHRTLEAFDASVAGLRNYFTLLQAAAGAAAAPAARQETGT"
     gene            3596..4246
                     /locus_tag="PLO65_08385"
     CDS             3596..4246
                     /locus_tag="PLO65_08385"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD28303.1"
                     /translation="MKRLILALVSAAALATAVQAAEPAFPDWRQYRDVANSSYVTPDG
                     GRVLQLSVVVKATPAQVWRAFTTGEGYREWVAPVARIDLAVDGMIEASYNAAARIGDP
                     DNIRNRIVAYVPERMLSIRNVQAPRQLPYRELFPQISTTIEMEDLGQGRTRVMLTAVG
                     YGQGEGFDTLYRHFEWGNAYTLDALKTRFETGPVDWAALEAKRKAAAATTVVQGGN"
     gene            4248..4586
                     /locus_tag="PLO65_08390"
     CDS             4248..4586
                     /locus_tag="PLO65_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28304.1"
                     /translation="MNRKALINATVIGTVLQLIMVLAGHWIPAVANLFAIVGILISLV
                     AGLLYARAAKGGWADSLLGGVIAGAVCALLGIAVSWLLGDVTPIILVIGTLSSAVGGL
                     IGGAIGKLLK"
     gene            complement(4634..5449)
                     /locus_tag="PLO65_08395"
     CDS             complement(4634..5449)
                     /locus_tag="PLO65_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRD28305.1"
                     /translation="MSTQDRISVTIEGGVADVRLVRVDKMNALDDAMFSALIETGERL
                     KTEPGVRAVVLSGDGRAFCAGLDMGNFGKMASGERRGGDSLVNTGRTEGGSNRAQHAV
                     MVWREIPVPVIAAVHGVAFGGGFQLTLGADIRYVAADTRLSVMEIKWGLVPDMAGLVL
                     MRHLARDDIVRELTYTGRQFSGEEAVQYGFATRVCADPRAEALALAHEIAGKNPHAIR
                     GDKRLLNLAASGADQHAVLLSESVEQGALIGSPNQIEAVMATMEKRAAKFAEA"
BASE COUNT          893 a         2051 c         1902 g          903 t
ORIGIN      
        1 ggcggcggca agccggtcaa ggtcgacatc cgggtgatcg ccacctccaa ccgcgacctg
       61 gcccaggccg tgcgcgaggg gactttccgc gaggacctgc tctatcgcct gaacgtcgtg
      121 aacctgcgcc tgccgccgct gcgcgagcgg cccggcgaca tcgtcgccct gtgcgatcac
      181 ttcgtgaaga aatacgccgc cgccaacggc gtgcccgagc gcccgctgtc ggccgaggcg
      241 cgccgccggc tggtcagcca tcgctggccc ggcaacgtgc gcgagctcga gaacgccatg
      301 cacagggcgg tgctgctgtc gaccggcgcg gagatcgagg agttcgccgt ccgcctgccg
      361 gacggccagc cgatgtcgcc ggcgcccgac gcggcgatgg cgcgcaacgc ctcgatggcc
      421 gccgacgcgg tcagccgggc cttcgtcggc cagaccgtgg cccaggtcga gcagcagctg
      481 atcctcgaca ccctcggcca ttgcctgggc aaccgcaccc atgcggcgac gatcctcggc
      541 atctccatcc gcacgctgcg caacaagctg aaggaatatt ccgaggccgg cgtcagcgtg
      601 ccggcgccgc aagccggcct gggggcgaac gcggcctagg gcgtcagccc ctaggtccga
      661 tccagcatgg cggacatcgg actctcagga ctggtcggcc aaaagccctc ggcgggaagc
      721 gtctggggct ggatggtgcg cggcgaggtc ggcctggccg tcggcgtcat cggcatcatc
      781 atcctgctga tcctgccgat accggccttc atgctggacg tcctgctgtc gctgtcgatc
      841 accagcgccg tgctgatcct gatgacggcc ctgctgatca agaagccgct ggaattcacc
      901 gcctttccga cggtgctgct ggtcaccacc ctgttccggc ttggcctgaa cctcgcctcg
      961 acccgactga tcctgaccca tggccacgag ggcgcccacg gcgccggcca gatcatcaag
     1021 gccttcggcg agctgatgat gggtggcaac tacatcatcg gggtgatcgt cttcgccatc
     1081 ctggtgatcg tgaacttcgt cgtcgtcacc aagggctcgg ggcggatcgc cgaggtcgcc
     1141 gcccgcttca ccctcgattc catgccgggc aagcagatgg ccatcgacgc cgacctgtcg
     1201 tcgggcctca tcgaccagga cgaggcgaag aagcgccgca aggacctgga gatggagtcg
     1261 accttcttcg gggccatgga cggcgccagc aaattcgtcc gcggcgacgc cgtggccggc
     1321 ctgatcatcg tcgccatcaa catcgtcggc ggcttcctga tcggggtcat ccagcacaag
     1381 atcccggccg gccaggccgc cacgacctac accctgatga ccatcggcga cggcctggtc
     1441 agccaggtgc cggccctgat catctcgatc gccgccggct tcctggtgtc caaggccggg
     1501 gtcgagggat cggccaacaa ggcgctggtc gcccagctgg cgatgaaccc gatcagcctg
     1561 ggcatggtct cggcctcggc cggcgtgatc gccctgatcc cggcgatgcc gaagataccc
     1621 ttcctggtcc tggccatcgc cgccggcgtt ctggcctggc gacggacggt caaggaaaat
     1681 gagccggagg tggtcatcga gaccgcgccg ccggccgagc aggaagagga gcccatctcc
     1741 cacgccctgg ccatcgacga cgtcaagatc gagctcggct acggcctgct ggcgctgatc
     1801 aacgatctcg acgggcgcaa gctgaccgac cagatccgcg ccctgcgcaa gacgctggcg
     1861 gccgacttcg gcttcgtcat gccgcccgtg cgcatcctcg acaacatgcg cctggcgacc
     1921 cagggctacg ccatccggat caaggagatg gagtgcggga cgggcgaggt gcgcatcggc
     1981 tcgctgatgg ccatggaccc gcgcggcggc caggtcgacc tgccggggga ccatgtgcgc
     2041 gagccggcct ttggccttcc ggcgacctgg atcgacgaga gcctcaagga ggaggcgacc
     2101 ttccgcggct ataccatcgt cgatccggcc acggtgctga ccacccacct gaccgagatc
     2161 ctcaaggaca acatggccga cctgctgtcc tacgccgagg tgcagaagct gctgaaggac
     2221 ctcgggccgg aacagaagaa gctggccgac gacgtggtcc cggccatcgt caccggaacc
     2281 agcctgcagc gggtgctgca ggcgctgttg cgggagcggg tgtcgatccg cgacctgccg
     2341 accatcctcg agggcgtggc cgaggccgcg ccgcacaccg cctcgatcac ccagctggtc
     2401 gaggcggtcc gcgcgcgact cggccgccag ctgtgctggg ccaaccgcgg cgacgacggc
     2461 gccctgccga tcgtcaccct gtccccggac tgggaggtcg ccttcagcga ggccctcgtc
     2521 ggtcagggcg aggacaagca actggccatg gccccgagca agctgcagga gttcatccgc
     2581 ggcgtccgcg aggccttcga gcgcgcggcc cttgccggtg acagcccggt cctgctgacc
     2641 agcccgcaga tccgcccgtt cgtgcgatcg atcatcgaac gcttccgcgg ccagaccgtg
     2701 gtgatgagcc agaacgaggt ccatccgcgg gcgcggctga agacggtggg gcaggtctag
     2761 ggagtagggc gtagggatta gggagtaggg cgtcgttcga ccctgcccgc tggccgtctc
     2821 gcgcctcgcc cttgctactc cctactccct actccctact ccctactccc tactccctac
     2881 tccctactcc ctatttccca cgccccatgc gttccacttg atcgaccgtt caacccgtgc
     2941 ttacttgatc attcaatcaa gaggcccgtc ccttggaacc gacccgcgag cgcatcctgt
     3001 tcgccgctat ggcgctcttc tcggagaagg gcttcctgtc gacctcggtg gccgacatcc
     3061 tgcgcgaggc gggagccaat tccggcagcc tgtaccactt cttcccgacc aagcaggacg
     3121 tgctgctggc ggtgcttgag atgtaccgcg acggcatcta tccgatgttg ctggccccgg
     3181 cctgggaggg cgtcgacgac ccgatcgagc gggtgttcgc ccttctggcc gcctatcgcg
     3241 gcatgctgaa aaacaccgcc tgcctgtacg gctgcccgat cggctcgatc gcccttgaac
     3301 tgcacgagcc cgacccgccg gtgcgggaga tgctgtcggt caatttcgag ggctgggtcc
     3361 gccatgtgaa gggctgcctg gacgccgcca aggcgcggct ccccgccgga accgacaccc
     3421 acgccctggc caccttcgtg ctgaccacca tggaaggcgg ggtcatgcag tcgcgcaccc
     3481 accgcacgct ggaggccttc gacgcctcgg tcgcgggcct gcggaactat ttcaccttgc
     3541 tgcaggccgc cgccggggcg gccgccgcgc ccgcggcgcg ccaggagacc ggaacatgaa
     3601 acgcctgatc cttgcgctcg tcagcgccgc cgccctggcc accgcagttc aggcggccga
     3661 accggccttt cccgactggc gacagtaccg cgacgtggcc aacagctcct atgtcacgcc
     3721 cgacggcggg cgggtgttgc agctgtcagt ggtggtcaag gccacgccgg cccaggtgtg
     3781 gcgggccttc acaacgggcg agggctatcg cgagtgggtg gcgccggtgg cccggatcga
     3841 cctggccgtc gatggcatga tcgaggccag ctacaacgcc gcggcccgga tcggcgaccc
     3901 ggacaacatc aggaaccgca tcgtcgccta tgtgcccgag cggatgctgt cgatccgcaa
     3961 cgtccaggcg ccgcgccagc tgccctaccg cgagctgttc ccgcagatct cgacgactat
     4021 cgagatggag gatctcggcc agggccgcac ccgggtcatg ctgaccgccg tcggctatgg
     4081 ccagggcgag ggcttcgaca cgctgtaccg ccacttcgaa tggggcaacg cctacacgct
     4141 cgatgccctg aagacccgtt tcgagaccgg cccggtcgac tgggcggcgc tcgaggcgaa
     4201 acgcaaggcc gcggccgcca cgaccgtcgt tcaaggggga aactgacatg aaccgcaagg
     4261 cgcttatcaa tgcgaccgtg atcggcacgg tgcttcagct gatcatggtc ctggccggcc
     4321 actggattcc ggccgtggcc aacctgttcg ccatcgtcgg catcctgatc tcgctggtcg
     4381 ccggcctgct ctacgcccgc gcggcgaaag gcggctgggc cgacagcctg ctgggcggcg
     4441 tgatcgccgg cgcggtctgc gccctgctgg ggatcgccgt gtcctggctg ctcggcgacg
     4501 tgacgccgat catcctggtc atcggcacac tgagctccgc tgtcggcggg ctgatcggcg
     4561 gcgcgatcgg caagctgctg aagtaacgga cggggacatg cacttcagtg cgtgtcccca
     4621 tcggcttctg acgtcacgcc tccgcgaact tcgccgcgcg cttctccatc gtcgccatga
     4681 ccgcctcgat ctggttgggg gagccgatca aggcgccctg ctcgacggac tcggacagca
     4741 gcaccgcgtg ctggtcggcg cccgacgcgg ccaggttcag caggcgcttg tcgccgcgga
     4801 tggcgtgcgg gttcttgccc gcgatctcgt gcgccagcgc cagggcctcg gcgcgcgggt
     4861 cggcgcagac gcgggtggcg aagccgtact gcacggcctc ctcgccggaa aactgccggc
     4921 cggtgtaggt cagctcgcgg acaatgtcgt cgcgggccag atgacgcatc agcaccagcc
     4981 cggccatgtc gggcaccagg ccccatttga tctccatgac cgacagccgg gtgtccgccg
     5041 cgacgtagcg gatgtcggcc ccgagggtca gctggaagcc gccgccgaag gccacgccgt
     5101 gcaccgcagc gatcaccggc acggggatct cgcgccagac catcaccgca tgctgggcgc
     5161 ggttcgagcc tccctcggtg cgaccggtgt tgaccaggct gtcgcccccg cgccgctcgc
     5221 ccgaggccat cttgccgaaa ttgcccatgt ccaggccggc gcagaaggcg cgaccgtcgc
     5281 ccgacaggac aaccgcccgc acgccgggtt ctgtcttcag ccgctcgccc gtctcgatca
     5341 gggccgagaa catggcgtcg tccagcgcgt tcatcttgtc gacccgcacc aggcgtacgt
     5401 cggccacgcc gccctcgatg gtcaccgaaa tccggtcctg cgtgctcatg gccaaggcct
     5461 ccctgcgcgt tttccggaag gtatcgggcc gggcgcggcg gccgcaaggg cgtgacccag
     5521 cggaacctcg gtcgctcccg gcggttaatt ggcccggacg ttgggggaag cacggagcac
     5581 gaacccatga acagcaaatc cattctcctc ggcctcagcg ccatcggcag cctggttctg
     5641 gccggcggcg cggcggcgca ggcgcttccc ggcgtgaccg gccaggtcgg cgtgaccggc
     5701 caggtgaccg ccccggcccc gacggttccg accgacaggg tcaccggcg
//
LOCUS       DAORNX010000161         5689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10492_length_5689_cov_7.857118, whole genome shotgun sequence.
ACCESSION   DAORNX010000161 DAORNX010000000
VERSION     DAORNX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10492_length_5689_cov_7.857118"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..2078
                     /locus_tag="PLO65_08400"
     CDS             9..2078
                     /locus_tag="PLO65_08400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HRD28306.1"
                     /translation="MKRVLLSAAAALALTCALGATPVLARADASPTAQADITRAPRMG
                     PWGFDLSGMDRTVKPGDDFVGFSSGSYLSRLEIPADRTSWGSFNALRELSDARIKAVI
                     DTSAATVNATGEAQQIGGLYNSFMDEARVEALDAKPLAGDLAAIRAATTRDDLARIMG
                     ATNGGFGGSFFAAYISQDAKDPNTNVTYLTQAGLGLPNRDYYLDPKFTEKKTAYQAYV
                     AQLLTLAGWADPAKAAADIVALETRIAEASWPIAEQRDADKTYNPKTVAELQALAPGF
                     PWQAWLNGASLDKAGTVIVGEPTAFTKIAAIYADAPVATLQAWQAFHTADQAAPYLSK
                     RFVDANFEFRARTLSGQPEQRARWKRGVTLVSNNLGEAVGKLYVDTYFPAESKAKMEK
                     LVADLRVALKARIDRLDWMSPETKTRAQAKLASFTVKIGYPNKWRDYSALTIKPDDLY
                     GNVQRATAFEWAFNVAKLGKPVDRDEWGMFPQTVNAYYNPTLNEIVFPAAILQPPFFD
                     PDADPAVNYAGIGAVIGHEISHGFDDEGRKFDADGSLRDWWQPADAAKFEAQTKRLGA
                     QYSAFEPVPGAKVNGDLTMGENIGDLGGILIALDAYHLSLGGKPAPVIDGLTGDQRFF
                     LGFAQIWRSKYRDDAIRQQVVSDPHSPPVFRVIGTLRNVDAWYDAWGVKEGDKWYVKP
                     EDRVRIW"
     gene            2254..4371
                     /locus_tag="PLO65_08405"
     CDS             2254..4371
                     /locus_tag="PLO65_08405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HRD28307.1"
                     /translation="MKTFWMSAAAAAALTFAAAPAMAHEQHACLDAFCFNQSLFAAAD
                     TTGGGETVSLESPRYGAWGFDLSGMDRSVKPGDDFYMWASGAWNARTEIPSDRSRFGN
                     FDALSILSEARTRAIIEDSAAGRITGPDAVKIGAAYAAFMDEARAESLDIKPLEPELA
                     AIRAVRTRDDMTALMGLSNVSGYSAIFGLGIGDDAKDPAHYAVYAGTGGLGLPDRDYY
                     LDPKFGPKKAAYEAYVAQMLTMAGWPKPAEAAKAIVAFETRLAEASWTRAERRDRDKT
                     YNPMTLAELQAATPGFDWNRYLAGTELKGVNRYVVSTNTAFPKFAAIYSETPVDTLKA
                     WQAFHLVDGAAPLLSKRFVDANFEFRSKTLAGQPEQKPRWKRGVAYVNGMLGESVGKV
                     YVARYFPAESKAKMEALVADIRAALKTRIEHLAWMSPETKVKALEKLSKFGVKIGYPD
                     KWRDYAALDVRADDAYGNAIRAGAFEWRYDVARLNGPVDKGEWGMTPQTVNAYYSSIK
                     NEIVFPAAILQAPFFHPDADMAVNFGGIGGVIGHEISHGFDDQGRKSDGDGVLRDWWT
                     AEDAAKFKTQADRLGAQYSAFEPYPGAKVQGALTMGENIGDMGGLSLAYDAYLVSLGG
                     KPAPILDGFTGQQRVFLGWAQVWRQKAREDFVRQQVVTDPHSPAYYRVNGTIRNIDAW
                     YDAFGVKDGDKLYVAPADRVRIW"
     assembly_gap    4431..4452
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4472..5059)
                     /locus_tag="PLO65_08410"
     CDS             complement(4472..5059)
                     /locus_tag="PLO65_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD28308.1"
                     /translation="MTSNPEDGYQPSNVTNARGRAVVITGCSGGGKSALLAELARRGH
                     RVFPEAGRQIVKEQDWIGGDVLPWSAPAKFAELVLSRGLHQRITAADAGGLAFFDRSV
                     VEPLAALERLALPVPSHFRRAAEVCRYHETVFVAPPWPEIYRADAERRHGFEEALAEY
                     GPLTAAYARLGYRLVELPKTTVAARAAFVLQTLGA"
     gene            complement(5504..>5689)
                     /locus_tag="PLO65_08415"
     CDS             complement(5504..>5689)
                     /locus_tag="PLO65_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRD28309.1"
                     /translation="GLVARGARPRANGLLIAERDLLEAQTLLAAGGIGPVAASRPEHL
                     FEPDSAAATALATRIGL"
BASE COUNT          961 a         2031 c         1867 g          808 t
ORIGIN      
        1 accccttcat gaaacgcgtc ctgctttccg ccgccgccgc gctggccctg acctgcgccc
       61 tcggcgccac gcccgtcctg gcccgcgccg acgcctcgcc gaccgcccag gccgacatca
      121 cccgcgcgcc gcgcatgggc ccgtggggct tcgacctttc gggcatggat cgcaccgtga
      181 agcccggcga cgacttcgtc ggcttctcgt cggggtccta tctgtcgcgg ctggagattc
      241 cggccgaccg gaccagctgg ggttcgttca acgccctgcg cgaactgtcc gacgcccgca
      301 tcaaggcggt gatcgacacc tcggcggcga cggtcaacgc gaccggcgag gcgcagcaga
      361 tcggcggcct gtacaacagc ttcatggacg aggcccgcgt cgaggcgctg gacgccaaac
      421 cgctggccgg cgacctcgcg gcgatccgcg ccgccaccac gcgtgacgac ctggcccgga
      481 tcatgggcgc gaccaacggc ggcttcggcg gcagcttctt cgccgcctac atcagccagg
      541 acgccaagga ccccaacacc aacgtcactt acctgaccca ggcgggcctg ggcctgccca
      601 accgcgacta ctacctcgat ccgaagttca ccgagaagaa gacggcctac caggcctacg
      661 tcgcccagct gctgaccctg gccggctggg ccgacccggc gaaggcggcg gctgatatcg
      721 tggcgctgga gacccgcatc gccgaggcca gctggccgat cgccgagcaa cgcgacgccg
      781 acaagaccta caaccccaag accgtcgcgg agctgcaggc cctggccccc ggcttcccgt
      841 ggcaggcctg gctgaacggc gcgagcctcg acaaggcggg cacggtcatc gtcggcgaac
      901 cgacggcctt cacgaagatc gccgccatct atgcggacgc cccggtggcg accctgcagg
      961 cctggcaggc cttccacacc gccgaccagg ccgcgcccta tctgtcgaag cggttcgtcg
     1021 acgccaattt cgagttccgc gccaggacgc tgtcgggcca gcccgagcaa cgcgcgcgat
     1081 ggaagcgagg cgtgacgctg gtcagcaaca acctcggcga ggcggtcggc aagctgtatg
     1141 tcgacaccta cttcccggcc gagtccaagg ccaagatgga gaagctggtc gccgacctgc
     1201 gggtggcgct gaaggcccgc atcgacaggc tcgactggat gagcccggag accaagaccc
     1261 gcgcccaggc caagctggcc agcttcacgg tcaagatcgg ctacccaaac aagtggcgcg
     1321 actacagcgc cctgaccatc aagccggacg acctgtacgg caacgtgcag cgcgcgaccg
     1381 ccttcgaatg ggccttcaac gtggccaagc tcggcaagcc ggtcgaccgc gacgagtggg
     1441 gcatgttccc gcagacggtc aacgcctact acaacccgac gctcaacgag atcgtcttcc
     1501 cggccgccat cctgcagccg ccgttctttg atccggacgc cgatccggcg gtcaactacg
     1561 ccggcatcgg cgcggtgatc ggccacgaga tcagccacgg cttcgacgac gagggccgca
     1621 agttcgacgc cgacggttcc ctgcgcgact ggtggcagcc ggccgacgcg gccaagttcg
     1681 aggcccagac gaaacgcctc ggcgcgcagt actcggcctt tgagccggtg cccggcgcca
     1741 aggtgaacgg cgacctgacg atgggcgaga acatcggcga cctgggaggc atcctgatcg
     1801 ccctggacgc ctatcacctg tcgctcggcg gcaagccggc cccggtgatc gatggcctga
     1861 ccggcgacca acggttcttc ctcggcttcg cccagatctg gcgtagcaag taccgcgacg
     1921 acgccatccg ccagcaggtg gtctccgacc cgcactcgcc gccggtgttc cgcgtcatcg
     1981 gcaccctgcg caacgtcgac gcctggtacg acgcctgggg cgtcaaggaa ggcgacaagt
     2041 ggtacgtcaa gccggaagac cgcgtccgga tctggtagcg cccttccaca cgccccttcg
     2101 gggctgctca ggatgacgta gctcggggca cgtcatggtg agcagcgccc ggagggtgcg
     2161 tggcgaacca cgcatgaaca atctttaacc tcccctcgcc gcccgggacg tgcaatctcc
     2221 ccgcccatgt ctggttccgg ggatttttcc gtcatgaaga ccttctggat gagcgccgcc
     2281 gcggcggctg ccctcacctt cgccgccgcg ccggcgatgg cccacgagca gcacgcctgc
     2341 ctcgacgcct tctgtttcaa ccagtcgctg ttcgcggccg ccgacaccac gggcggcggc
     2401 gagacggtgt cgctggagag cccgcgttac ggcgcctggg gcttcgacct gagcggcatg
     2461 gaccgttcgg tgaagccggg cgacgacttc tacatgtggg ccagcggcgc ctggaacgcg
     2521 cgcaccgaga ttccgtcgga ccgctcgcgg ttcggcaact tcgacgccct gtcgatcctg
     2581 tccgaggccc gcacccgggc gatcatcgag gactccgcgg ccggtcgcat caccggtccc
     2641 gacgcggtca agatcggcgc ggcctatgcc gccttcatgg acgaggcgcg ggccgagagc
     2701 ctcgacatca agccgcttga gcccgagctg gccgccatcc gggcggtcag gacccgcgac
     2761 gacatgaccg ccctgatggg cctgtccaac gtctccggct acagcgccat ctttggcttg
     2821 ggcatcggcg acgacgccaa ggacccggcc cactacgcgg tctacgcggg caccggcggg
     2881 ctggggctgc cggaccggga ctattacctc gatcccaagt tcgggccgaa gaaggcggcc
     2941 tacgaggcct atgtcgccca gatgctgacc atggccggct ggccgaagcc ggccgaggcc
     3001 gccaaggcca tcgtggcctt cgaaacccgt ctggccgaag cgtcctggac gcgggccgag
     3061 cgccgcgacc gcgacaagac ctacaatccg atgaccctgg cggaactgca ggcggcgacg
     3121 ccgggcttcg actggaaccg ctacctggcc gggaccgagc tgaagggcgt caaccgctac
     3181 gtcgtcagca ccaacaccgc cttcccgaag ttcgcggcga tctattcgga aacgccggtc
     3241 gacacgctca aggcctggca ggccttccat ctggtcgacg gcgccgcgcc gctgctgtcg
     3301 aaacgcttcg tcgacgccaa cttcgagttc cgctcgaaga ccctcgccgg ccagcccgag
     3361 cagaagccgc gctggaagcg cggcgtcgcc tacgtcaacg gcatgctcgg ggagtcggtc
     3421 ggcaaggtct atgtcgcccg ctacttcccg gccgagtcca aggccaagat ggaggcgttg
     3481 gtcgccgaca tccgcgcggc gctgaagacc cgcatcgagc acctcgcctg gatgagcccc
     3541 gagaccaagg tcaaggcgct ggagaagctg tccaagttcg gggtcaagat cggctatccg
     3601 gacaagtggc gcgactacgc ggccctcgat gtccgcgccg acgacgccta tggcaacgcc
     3661 atccgcgccg gcgccttcga atggcgctac gatgtcgccc gcctcaacgg cccggtcgac
     3721 aagggcgaat ggggcatgac cccgcagacg gtcaacgcct actacagctc gatcaagaac
     3781 gagatcgtct tcccggccgc catcctgcag gcgccgttct tccatccgga cgcggacatg
     3841 gcggtcaact tcggcggcat cggcggagtg atcggccacg agatcagcca cggcttcgac
     3901 gaccagggcc gcaagagcga cggcgacggc gtgctgcgcg actggtggac cgccgaggac
     3961 gcggccaagt tcaagaccca ggcggaccgc ctcggcgccc agtactcggc cttcgagccc
     4021 tatccggggg ccaaggtcca gggcgccctg accatgggtg agaacatcgg cgacatgggc
     4081 ggcctgtcgc tggcctacga cgcctatctg gtgtcgctgg gcggcaagcc cgcgccgatc
     4141 ctcgacggct tcaccggcca gcagcgggtg ttcctcggct gggcccaggt ctggcgccag
     4201 aaggcccgcg aggacttcgt ccgccagcag gtggtcaccg atccgcactc gccggcctac
     4261 taccgcgtca acggcacgat ccggaacatc gacgcctggt acgacgcctt cggggtcaag
     4321 gatggcgaca agctgtacgt cgcgccggcc gaccgggtca ggatctggta ggaaccacgc
     4381 ggggacatgc acttcagtgc gtgtcccctg ccaaccaccg agagggggac nnnnnnnnnn
     4441 nnnnnnnnnn nnccccgcat ccgcgcccgc ctcacgcccc cagcgtctgc agcacgaagg
     4501 ccgcccgcgc cgcgaccgtg gtcttcggca gctcgaccag ccggtagccc agcctggcgt
     4561 aggccgccgt caggggaccg tattccgcca gggcctcctc gaacccgtgc cggcgctcgg
     4621 cgtctgcgcg gtagatctct ggccagggcg gggcgacgaa gaccgtctcg tggtagcggc
     4681 aaacctcagc ggcccgccgg aagtgcgacg gaaccggcag ggcgagccgc tcgagcgcgg
     4741 ccagcggctc gacgacgctg cggtcgaaga aggccaggcc gccagcgtcg gcggcggtga
     4801 tccgctgatg cagcccgcgc gacaggacca gctcggcgaa cttcgcgggc gccgaccagg
     4861 gcaggacgtc gccgccgatc cagtcctgct ccttgacgat ctgccgaccc gcctcgggga
     4921 acacccgatg gccgcgccgc gccagttccg ccagcagggc agatttgccc cctccggagc
     4981 agcccgtgat gaccacggcc cggccgcggg cgttggtgac gttcgacggc tgatagccgt
     5041 cctcggggtt tgaggtcatg acgtctcctg aggagtgtgg ggatgtcggg ggtgcgtggc
     5101 tcgacccgcg cccgccgggc gccgctcacc atgacgcgca cggggcgacg tcatcctgag
     5161 caaccacgaa gcggcgggtc gcagggcgca gggtattggg ggtcggagat ttttgacggc
     5221 cgcgccgaaa aacacagacc cgccgcgggg gatagtcgcg gcgggtccgg tttattcctc
     5281 gatcactcga tccaagcggg cgtttcctcc ccgaaacgcc gtgagggcag acctctctgg
     5341 gggtcgtgtc cctcgcgcaa catcaaaaga cggcaattac ctttccctgt caaggcagct
     5401 ggccggggga gcgcgcaaca aattacgccg aaatggcgaa aaacgggtcg tttcttggac
     5461 tgacgagtca aaagaaccgg aagttttgac tccatcgtca aaattataac ccgatccggg
     5521 tggccagcgc cgtggccgcc gccgagtccg gctcgaacag gtgctccggg cgactcgcgg
     5581 ccaccgggcc gattcccccg gcggccagca gtgtctgggc ctcgagcagg tcccgttcgg
     5641 cgatcagcag accgttggcc cggggccgcg ccccgcgggc caccagtcc
//
LOCUS       DAORNX010000162         5684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10512_length_5684_cov_7.779890, whole genome shotgun sequence.
ACCESSION   DAORNX010000162 DAORNX010000000
VERSION     DAORNX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_5684_cov_7.779890"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PLO65_08420"
     CDS             complement(<1..107)
                     /locus_tag="PLO65_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD28310.1"
                     /translation="MKIAFIGLGVMGFPMAGHLAKAGHEVSVFNRGPAKA"
     gene            308..1216
                     /locus_tag="PLO65_08425"
     CDS             308..1216
                     /locus_tag="PLO65_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRD28311.1"
                     /translation="MALGSDAPVMIKKVKKVVGGGHHGGAWKVAYADFVTAMMAFFLL
                     MWLINTTSPEQKRGIADYFAPASVAETTSGSGGILGGTALGDDGVKSSGSMATVEQMA
                     PEAPPQTKTTGQSDTSPGATDLESASEEALRQELQAREDAAFASAAQSLRQALQDMPE
                     LAELSKQILIDQTPEGLRIQLVDQDGRSMFKDGAAMPNDRARILLRAVSRIINQLPNR
                     ISISGHTSISPNGRTASSDWALSSARADVARQLLQGFGVNADRVYQVAGKANSDPLYP
                     DDPMLAGNRRISIILLREAPVLPPDR"
     gene            1307..2131
                     /locus_tag="PLO65_08430"
     CDS             1307..2131
                     /locus_tag="PLO65_08430"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRD28312.1"
                     /translation="MTHHFTSRRMRFFLTAPSPCPYLPGREERKVFAHLPASDGPAVN
                     DSLTQSGFRRSQHIAYRPACESCSACASARLPVMDYVFSRSERKTLARNSDLRRHLVE
                     SEATMEQFDLLRRYLLARHADGGMAEMTWPDYVAMVEDTAVRTHIIEYRGPSTDDGPG
                     DLIACVLIDVMSDGLSMVYSFYDPRLVKRSLGTFIILDHVVQAGLNGLAYVYLGYWVP
                     GSEKMAYKARFSPLEVLRPQGWMLISARDRAEAGESLARERRLRGPVVARLGSKAP"
     gene            2133..2600
                     /locus_tag="PLO65_08435"
     CDS             2133..2600
                     /locus_tag="PLO65_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28313.1"
                     /translation="MFDIREDDLSGGDVRALLAIHLAGMAENTPSGHVFALDLSGLRS
                     PEITVWTVRDDGALAGIGALKALGDGTGELKSMRTHPDHLRRGVAAALLDHILDEARA
                     RGYRRLSLETGVGESFEPALALYRRRGFGNGEAFADYRPSAFNQFLHLDLCGP"
     gene            complement(2597..3592)
                     /locus_tag="PLO65_08440"
     CDS             complement(2597..3592)
                     /locus_tag="PLO65_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRD28314.1"
                     /translation="MGAVGLYTHIQSNNLRSALLLAGFPVLLLGLTYALTLGLIGLGL
                     LPSTGSIDGDLAEAAGFMLAGAPPALIVTAVWFVVAYFGNQVIIDLATGARPATRQAE
                     PDLYNLLENLCISRGLPMPSLRIIESDALNAFATGLNEKQYSITVTRGLMTVLDRAEL
                     EAVLAHELTHVINRDVRTMVIASVFAGVITLVAEILFRSIRFGGLGGRRGDRKGAGLF
                     ILVAFVAAAVGYVLAIVIRMSLSRSREFVADAGSVELTKNPDAMISALRKIEGHSALP
                     APESVQGMFIENREEGIYALFATHPPISKRIEALVRFAGGHDSPNPIVAGPGPWG"
     gene            complement(3603..4160)
                     /locus_tag="PLO65_08445"
     CDS             complement(3603..4160)
                     /locus_tag="PLO65_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRD28315.1"
                     /translation="MTLIIILVVIVLVGLMAVGLYNRLVALNTRADQAFADIDVQLRQ
                     RHDLIPNLVETVKGYATHEKGTLEAVIAARNAATSAGSVADKVQAENALTGALRQMFA
                     VAEAYPELKANTNFLSLQSELSDVENKLAAARRFFNSAVAEFNAARLSFPAVLFAGMF
                     GFNARALFDLGEQRAAMDAAPEVKF"
     gene            complement(4193..5524)
                     /locus_tag="PLO65_08450"
     CDS             complement(4193..5524)
                     /locus_tag="PLO65_08450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD28316.1"
                     /translation="MIWRKSLTAAVAVLVLAGSAPATAAAPWSRKAAPAASAPAQGLP
                     TATPESVGFSSERLRRLHGVMDAFVDRGFAPGVATVVIRHGRIVDSHIYGKADLATGA
                     PLAQDTLWRMYSQTKPVTGVAMMILYEEGKWRLDEPVTKYVPEFAGLKVFKGMDKDGK
                     PILEPIARSATMRELMTHTAGFAYGLVDDNYVDKLYRDSLTSGWPNLTSAMTAISALP
                     LASQPGTEWKYSLAVDIQGYIVEKLSGQTLPDFMQSHIFGPLGMTDTAFWVPPAKQGR
                     LASLYMFDRETRKIVPADSFMVLPMDKVPTAPNGGGGLVSTMADYSRFAAMLAGGGEL
                     DGVRILSPSTIRLMASNHLTDATLATYQANNPGHGFGLDVDVIMDPAKAGTTAGKGSF
                     SWGGAAGTWFWVDPENDVVVLGFIHVLGGSFYRLDERSATLVYQALVDPAK"
BASE COUNT          900 a         1969 c         1933 g          882 t
ORIGIN      
        1 gccttggccg ggccgcggtt gaacacgctg acctcatgcc cggccttcgc cagatggccc
       61 gccatcggga agcccatgac cccaaggccg atgaaggcga ttttcatgtc cgaattctcc
      121 gttgcgtcct tcgacacggc cctgcgggcc tgctcaggat gacgtgcaga tgtgacgctt
      181 caagacccca cgtcatggtg agcagcgctc gaaagcgcgc gtgtcgaacc acgcaacctg
      241 ccggatcgca accccatctt aacgcccgcc cgccaccttc cgagtcacgg ttaagcgggg
      301 attcaccatg gccctgggca gcgacgcgcc cgtcatgatc aagaaggtca agaaggtcgt
      361 cggcggcgga caccacggcg gcgcctggaa ggtcgcctac gccgacttcg tgaccgccat
      421 gatggccttc ttcctgctga tgtggctgat caacaccacc agccccgagc agaagcgcgg
      481 catcgccgac tacttcgccc ccgccagcgt ggccgagact accagcggtt cgggcggcat
      541 tctcggcggc accgccctcg gcgacgacgg ggtcaagtcg tccggctcga tggccaccgt
      601 cgagcagatg gcccccgagg ctccgccgca gaccaagacg acaggccaga gcgacaccag
      661 ccccggcgcg accgatctgg agtcggccag cgaggaggcc ctgcgccagg aactgcaggc
      721 ccgcgaggac gccgccttcg cctcggccgc ccagtccctg cgccaggcgc tgcaggacat
      781 gcccgagctg gccgagctgt cgaagcagat cctcatcgac cagacgccgg agggcctgcg
      841 catccagctg gtcgaccagg acggccgctc gatgttcaag gacggcgcgg ccatgcccaa
      901 cgaccgcgcc cgcatcctgc tgcgcgcggt gtcgcggatc atcaaccagc tgcccaaccg
      961 gatctcgatc agcggccaca ccagcatcag cccgaacggc cgcaccgcct cgagcgactg
     1021 ggccctgtcc tcggcgcggg ccgatgtcgc ccgccagctg ctgcagggct tcggggtcaa
     1081 tgcggaccgg gtctaccagg tcgccggcaa ggccaattcc gatccgcttt acccggacga
     1141 tccgatgctc gcgggaaacc ggcgcatctc gatcatcctg ttacgcgaag cccctgtcct
     1201 tccccccgat cggtgaccca tgatcggtca aaccccgctt gcacggccgc gcgtcctgct
     1261 tcctaatcgt tacggggtag aggagagccg caaggcatag gggcccgtga cgcatcactt
     1321 cacatcgcga cggatgcgtt ttttcctcac ggccccctcg ccgtgcccct atcttccggg
     1381 ccgcgaggaa cgcaaggtct tcgcccatct gccggcctcg gacggtccgg cggtcaacga
     1441 cagcctgacc cagtcgggct tccgccgcag ccagcatatc gcctaccgcc cggcctgcga
     1501 atcctgctcg gcctgcgcct cggcgcgcct gccggtgatg gactacgtct tctcccgcag
     1561 cgagcggaag accctggcgc gcaacagcga cctgcgccgg catctggtcg agtccgaggc
     1621 gaccatggag cagttcgacc tgcttcgccg ctacctgctg gcccgccacg ccgacggcgg
     1681 catggccgag atgacctggc ccgactatgt cgccatggtc gaggacaccg cggtccgcac
     1741 ccacatcatc gagtatcgcg gcccgtcgac ggacgacggc ccgggcgacc tgatcgcctg
     1801 cgtgctgatc gacgtgatga gcgacggcct gagcatggtc tacagcttct atgacccgcg
     1861 gctggtcaag cgcagcctcg gcaccttcat catcctcgac catgtggtcc aggccgggct
     1921 caacgggctg gcctatgtct acctgggcta ctgggtcccg ggcagcgaga agatggccta
     1981 caaggcccgc ttctcgccac tggaggtgct gcggccgcag ggctggatgc tgatctccgc
     2041 ccgcgaccgc gccgaggccg gggagagtct ggcccgcgaa cgccgcctgc gcgggccggt
     2101 cgtggccagg ctgggcagca aggcgcccta gggtgttcga catccgcgag gacgacctct
     2161 ccggcgggga cgtgcgcgcc ttgctggcga tccatctcgc cggcatggcc gagaacactc
     2221 cgtccgggca tgtgttcgcg ctcgacctgt cgggcctcag gtcgcccgag atcacggtct
     2281 ggaccgtgcg ggacgacggc gcgctggccg gtatcggggc cctcaaggcg ctgggcgacg
     2341 gaaccggcga gctcaagtcg atgcgcaccc atcccgacca cctgcgccgg ggcgtcgccg
     2401 ccgccctgct cgaccacatc ctcgacgagg cgcgcgcccg cggctatcgg cgcctgagcc
     2461 tcgagacggg cgtgggagag agcttcgaac cggccctggc cctctatcgc cggcgcggct
     2521 tcggcaacgg cgaggccttc gccgactacc ggcccagcgc cttcaaccag ttcctgcatc
     2581 tcgacctctg tgggccctag ccccaggggc ccggcccggc cacgatcggg ttcgggctgt
     2641 catggccgcc cgcgaacctg accagcgcct cgatgcgctt gctgatcggc ggatgggtgg
     2701 cgaacagggc gtagatcccc tcctcccggt tctcgatgaa catgccctgg acagattccg
     2761 gcgccggcag ggcgctgtgg ccctcgatct tgcgcagggc ggagatcatc gcgtcggggt
     2821 tcttcgtcag ctcgaccgaa ccggcgtcgg cgacgaactc gcggctgcgc gacaggctca
     2881 tgcggataac gatggccagg acatagccga ccgccgccgc gacgaaggcg accaggatga
     2941 acaggcccgc ccccttgcgg tcgccgcgcc gtccgccgag cccaccgaag cggatcgagc
     3001 ggaacagaat ctcggccacc agggtgatca ccccggcgaa caccgaggcg atgaccatgg
     3061 tgcggacgtc gcgattgatc acatgggtca gctcgtgggc cagcaccgcc tccagctcgg
     3121 cccggtcgag caccgtcatc aggccgcggg tgacggtgat gctgtactgt ttctcgttca
     3181 ggccggtggc gaaggcgttg agcgcgtcgc tctcgatgat ccgcaggctc ggcatcggca
     3241 ggccgcgcga gatgcacagg ttttccagaa ggttatagag gtcgggctcc gcctggcggg
     3301 tcgccggacg ggcgccggtg gccagatcga tgatcacctg gttgccgaag taggcgacca
     3361 cgaaccagac ggccgtcacg atcagcgccg gcggcgcgcc ggccagcatg aacccggccg
     3421 cctcggccag gtcgccgtcg atcgaaccgg tcgagggcag caggccaagg ccgatcagtc
     3481 cgagcgtcag ggcgtaggtc aggccaagca gcagcacggg aaaccccgcc agcagcaggg
     3541 ccgagcgcag gttgttgctc tggatatggg tgtagaggcc gacggccccc atgacgcggt
     3601 tcctagaact tcacttcggg cgcggcgtcc atcgccgccc gttgctcgcc caggtcgaac
     3661 agtgcgcggg cgttgaagcc gaacatcccc gcgaacagga cggccgggaa cgacagccgc
     3721 gcggcgttga actcggccac cgcgctgttg aagaagcggc gggcggcggc cagcttgttc
     3781 tcgacgtccg acagctcgct ctgcaggctc aggaagttgg tgttggcctt gagctccgga
     3841 taggcctcgg cgacggcgaa catctgccgc agggcgccgg tcagggcgtt ctcggcctgg
     3901 accttgtcgg ccaccgagcc ggcgctggtc gccgcgttgc gggcggcgat gaccgcctcc
     3961 agcgtaccct tctcgtgcgt ggcgtagccc ttcacggtct cgacgaggtt ggggatcagg
     4021 tcgtggcgct gcctgagctg cacgtcgatg tcggcgaagg cctggtcggc gcgggtgttg
     4081 agcgcgacca gccggttgta cagcccgacc gccatcaagc cgaccaggac gatgacgacg
     4141 aggatgatga tcagggtcac gggcgcctcc ggttgtcccg cgaaggctag ccctacttcg
     4201 ccggatcgac cagcgcctgg tagaccagcg tcgcggaacg ctcgtccagg cggtagaagc
     4261 tgccgcccag cacatggatg aagccaagca ccacgacgtc gttttccgga tcgacccaga
     4321 accaggtgcc ggccgccccg ccccagctga acgagccctt gccggccgtc gtgccggcct
     4381 tcgccgggtc catgatgacg tcgacatcaa ggccgaagcc gtggccgggg ttgttcgcct
     4441 gataggtggc cagggtcgcg tcggtcaggt ggttggaggc catgagccgg atcgtcgacg
     4501 ggctgaggat acgcacgccg tcaagttcgc ccccgcccgc cagcatggcc gcgaaacggc
     4561 tgtagtcggc catggtcgag accaggccgc cgccgccgtt cggcgcggtg gggaccttgt
     4621 ccatcggcag caccatgaag ctgtcggccg ggacaatctt ccgcgtctcg cggtcgaaca
     4681 tgtacaggct ggccaggcgg ccctgcttgg cgggcgggac ccagaaggcg gtgtcggtca
     4741 tgccgagcgg gccgaagata tggctttgca tgaagtcggg cagggtctga cccgacagct
     4801 tctcgacgat gtagccctgg atatcgacgg ccagggagta tttccattcc gtgcccggct
     4861 ggctggccag cggaagggcc gaaatcgcgg tcatggcgct ggtgaggttc ggccagccgc
     4921 tggtcaggga gtcgcggtag agcttgtcga catagttgtc gtcgaccagg ccgtaggcga
     4981 agccggcggt gtgcgtcatc agctctcgca tggtcgccga ccgggcgatc ggctcgagga
     5041 tcggcttgcc gtccttgtcc atgcccttga acaccttgag gccggcgaac tcggggacgt
     5101 acttggtcac cggctcgtcg agacgccact tgccctcttc atagaggatc atcatcgcca
     5161 cgccggttac cggcttggtc tggctgtaca tccgccacag ggtgtcctgg gccagcggtg
     5221 cgccggtggc gaggtcggcc ttgccgtaga tgtggctgtc gacgatccgg ccgtggcgga
     5281 tgaccaccgt ggcgacgccg ggggcgaagc cgcgatcgac gaaggcgtcc atgacgccat
     5341 gcaggcggcg caggcgttcc gacgagaagc cgaccgactc gggcgtggcc gtgggcaggc
     5401 cctgggccgg agccgaggcg gccggcgcgg ccttgcggct ccagggcgcc gctgccgtcg
     5461 ccggcgccga accggccagc accagcacgg ccacagccgc ggtcagactc ttgcgccaga
     5521 tcatcattcc atcccccagt ccggcgctcg aacggccgtt tgacggcaag gtaacgcggg
     5581 ttcgcggcgg ggcaagacgg acgtggggct tcgggcaggg tttcgcgcgc cccgctgtca
     5641 tccagcgcgg tggggaggtg gtgcggcgcg ctccggtctg cccg
//
LOCUS       DAORNX010000163         5620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10650_length_5620_cov_7.154178, whole genome shotgun sequence.
ACCESSION   DAORNX010000163 DAORNX010000000
VERSION     DAORNX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10650_length_5620_cov_7.154178"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLO65_08455"
     CDS             complement(<1..266)
                     /locus_tag="PLO65_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28317.1"
                     /translation="MTYQDPTDLYGPPPDFRRDRLRAAFITSLVALATDMPAREIAAP
                     TRCSQAATRARQLAIYLAHVTLSWPLARVALAFGRDRTTAGAAVR"
     gene            437..1087
                     /locus_tag="PLO65_08460"
     CDS             437..1087
                     /locus_tag="PLO65_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28318.1"
                     /translation="MKFTLTYDGELPSGGNGSKVAKKAKWAIREQFHPQLVELWGLNP
                     ALKRLPLHSIVPKSGTFPWVEGHHFGEEGVLPPDFKMADDEIDLCALIERGADKYKPI
                     VRNSYALTCSLNILFLRKEAAGRVYQGGDLDNRIKTLLDALSVPHIYHPRPPHLAGVD
                     PMHCLLEDDALVTGLNVKSERLLSRPGTSENEVHLVVEVDVRVSDTRNYNAMFLGD"
     gene            1247..1564
                     /locus_tag="PLO65_08465"
     CDS             1247..1564
                     /locus_tag="PLO65_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28319.1"
                     /translation="MAFETTVNVEYKHRDGWHVFTSQDALGLYVASKDARTAYEDVPG
                     ALKMLMELDYSCQCEVSRAIPFEQFAEKVLRGNDESTSPPELRNESLHVRGCRNDSVV
                     GPL"
     gene            complement(1773..1961)
                     /locus_tag="PLO65_08470"
     CDS             complement(1773..1961)
                     /locus_tag="PLO65_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28320.1"
                     /translation="MKPKKPTLEDEDQSRRFLEAARALEADGGLNPTEGEAAFEQLLK
                     GAAPARLPEKKPDPDKAG"
     gene            complement(1964..2914)
                     /locus_tag="PLO65_08475"
     CDS             complement(1964..2914)
                     /locus_tag="PLO65_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRD28321.1"
                     /translation="MSNLSAPHFHNEEAAYAYVEARVWPQGAVCPHCGGYERIGKMGG
                     KSTRIGTYKCYQCRKPFTVKVGTIFEASHVPLRHWLQAMYLMAASKKGISANQIHRTL
                     GCTLKTAWFLAHRIREAMRSGDLAPFGEGGPVEMDETYQGNIKEPKTVRSNGTPFLGS
                     KRGRGPSNKRTIVALVERGGRVRSFHIERATKSSINEIILANVAREAVLYTDESRLYA
                     DAKDHVSEHASVRHSRGEYVVAAAHSNTIEGVFSIFKRGMRGVYQHCSEKHLHRYLAE
                     FDFRYCNRVALGVNDIERTDKLLSGVVGRRLTYQTARGQA"
     gene            complement(3099..3590)
                     /locus_tag="PLO65_08480"
     CDS             complement(3099..3590)
                     /locus_tag="PLO65_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRD28322.1"
                     /translation="MAAQLGASATAARAHEELFAYWASLRQAGRLPGRADIRPEDFKR
                     LLPTVSLIDIVEGPRRDYRLRLAGTGLYSVYGREITGRSLGDVYGAKAADYWRAELDK
                     VVEGRRPGVGCHSLAWRGAGHMSLLWLRLPLASDGRNVDMILGYDAVVGVHGDTSSGI
                     RAA"
     gene            complement(3793..4212)
                     /locus_tag="PLO65_08485"
     CDS             complement(3793..4212)
                     /locus_tag="PLO65_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRD28323.1"
                     /translation="MEDKSAIIEMTTDIVSAYVGNNSVAANDIPGLIQNVHKALTDVA
                     SGSVTVEVAPKEPAVPVRRSITPDHLVCLEDGRKFKSLKRHLRTKYNMSPEEYRAKWG
                     LPKDYPMVAPNYAKARSDLAKQMGLGQGGRQAAKKKR"
     gene            complement(4426..>5620)
                     /locus_tag="PLO65_08490"
     CDS             complement(4426..>5620)
                     /locus_tag="PLO65_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HRD28324.1"
                     /translation="DPAPAPAPARSRATLLKRLADVVCLPGSRVNAFERAMTADLLIE
                     LLREAAIDERVRIARRIANVSEIPTSLARLLMRDDVEVARALLENSATVGDGDLIDCV
                     RSATVEHRRLIAARRGLNEVVADELVRQGEPAVVETLLRNDLARLSHEAIETIVAGTR
                     DNPRHIPLLLRRGELRPSHAYVMFWWSDAEARRIILQRFAVSREVLQEAVSDVFAMAA
                     DEGWQDPLTRKALQFIERRQRNRAAIAKSPFESLDEAVMVASESMTREVAEEISYLSG
                     LKPMTGAKIFTDPGGEPLAILCKATGLPRNNLRLLWRALRRPETDSAGNLAPALERVL
                     TLFDTIAVDRAQTVLRYWNWSLSSALTPALIQAIREGDEQALDEFSQPQRAAMLALAG
                     DLRRR"
BASE COUNT          990 a         1786 c         1806 g         1038 t
ORIGIN      
        1 cgcaccgccg cgccggcggt ggtccggtcg cggccgaagg cgagcgcgac ccgggcgagc
       61 ggccaggaca gggtcacatg ggccaggtag atggccagct ggcgcgcccg ggtggcggcc
      121 tggctgcagc gggtcggcgc tgcgatctcc cgggccggca tgtcggtcgc cagggccacc
      181 aggctggtga tgaaggccgc ccgcagccgg tccctgcgga agtccggcgg cgggccatag
      241 agatcggtgg ggtcctgata ggtcatgatg tcctcgcgcc gcgggcggat ccgtcggctc
      301 ttcagcgctg attgtggcca gcaccggagc atggaggata atattggcgt tatgtgactt
      361 cccgtaccca tccttgccgt gaggcgggga cttgctaagc tcacgcagca ttggcttggc
      421 tggggggctt agcgtcatga agttcactct cacctatgac ggtgagcttc cgtctggcgg
      481 taacggctcc aaggtcgcca agaaggcgaa gtgggcgatc cgggaacaat tccatccgca
      541 actggtggag ctgtggggcc taaacccggc actgaagcgg ctgccccttc attctatcgt
      601 cccaaagtcc ggcacgttcc cgtgggttga gggtcaccac ttcggagaag agggcgttct
      661 accccccgac ttcaaaatgg ccgatgacga gattgatctc tgtgcgctga tcgagcgggg
      721 ggccgacaag tacaagccca tcgtccgaaa ttcatacgcc ctgacatgca gcctcaacat
      781 ccttttcttg aggaaggagg cggcggggcg ggtgtatcag ggcggcgacc tggataaccg
      841 gatcaagacg ctgctcgacg ctctctcagt tcctcacatc taccatccaa ggccgcccca
      901 cctcgccggg gtcgacccca tgcattgctt gctggaggac gatgccttgg tgacgggtct
      961 gaacgtgaag tcagaacgcc tcctgtcccg ccccggcacc tcggaaaatg aagtccacct
     1021 agtggttgag gtggacgttc gcgtctcaga tacgcgcaac tacaacgcta tgtttctcgg
     1081 tgattgatcc gttcccgtgg gacgcgagga aggggggcat aaagctcccg ttatgcgacg
     1141 cttgcgaatc ctgcgcgtta aatacatctt gacaactgtt cgcctcattc tgcgttaagg
     1201 tccccggggt ggcgcaaatg cgttttgttg cgcctaaagg tagaaaatgg cattcgaaac
     1261 aaccgttaac gtcgaataca agcaccgcga cgggtggcat gtgttcactt cacaggacgc
     1321 tctcggcctc tatgtcgcga gcaaagacgc ccgcaccgcc tacgaggatg ttccgggcgc
     1381 tctgaaaatg ctcatggaac tcgactattc ctgccagtgc gaagtttcgc gcgccattcc
     1441 gttcgagcaa ttcgcggaaa aggtcttgcg cgggaacgac gaatcaacgt cgcctcccga
     1501 gcttcgcaat gaatctctcc acgtgagggg ctgcaggaat gatagcgtcg tcggtcctct
     1561 ctgaggatag tttgaaggcc gccttggcgg cccgcgactg cgttcaaacc gagcacaaaa
     1621 cccagaccgg gacattttgg aagacccgac aggggctgca cttcctcgtg ccccgttcgc
     1681 ttgatggctt ctacgctgac tggatgctct gggacttgga gcgggtcatc gggaagatcg
     1741 aggcacagtc agcctttaac cggaagcagt agctacccgg ccttgtccgg gtcaggcttc
     1801 ttctcaggca acctcgccgg ggctgccccc ttcagcaact gctcaaaagc ggcttcgcct
     1861 tcggtggggt ttagtccgcc atcggcctct aatgcgcggg cggcttccag aaagcgccga
     1921 ctctgatcct cgtcctctag agtcggcttc ttcggtttca ttgtcaggcc tgcccacgag
     1981 ctgtttgata ggtcagccgc cgaccgacga caccggacag cagcttatca gtgcgttcga
     2041 tgtcgttcac gcccaaagca acgcggttgc aatagcggaa atcgaactcg gccagatacc
     2101 ggtgcaggtg cttttccgag cagtgctgat agacgccgcg catcccgcgt ttgaaaatgg
     2161 agaacacccc ttcgatggtg ttggagtggg cggcggcgac gacatattcg ccccggctgt
     2221 gccgcacgct ggcatgttcg gacacgtggt ccttggcgtc cgcgtacagg cggctttcgt
     2281 cggtatagag gacggcctcg cgcgccacgt tcgcgagaat gatctcattg atgctggact
     2341 tagtggcgcg ctcgatgtgg aacgagcgaa cccgtccgcc gcgctcaacg agagcgacga
     2401 tggtacgctt gttggacggg ccgcgcccgc gcttgctccc gaggaaggga gtgccattgg
     2461 agcgaaccgt cttcggctcc ttgatgttgc cttgataggt ctcgtccatc tcgaccgggc
     2521 caccttcgcc gaacggggcc aggtcgccgg aacgcatggc ttcgcggatg cggtgagcaa
     2581 gaaaccacgc ggttttcagg gtgcagccga gcgtccggtg gatctggttg gcgctgatgc
     2641 ccttcttcga cgccgccatg aggtacatgg cctgcaacca gtggcgcagg ggaacgtggc
     2701 tggcctcgaa gatcgtccct accttcacgg tgaagggctt gcggcactgg tagcacttgt
     2761 aagtgccgat ccgggtggac ttgccgccca tcttcccgat ccgctcatac cctccgcagt
     2821 gcgggcaaac agcgccctgc ggccagaccc gcgcctcaac gtaggcgtag gcggcttctt
     2881 cgttatggaa gtgcggggcg gacaggttgg acacggcgta actccttatg gcgcttacct
     2941 tacagcatca tttgggtacg tcaagtataa catcgccata atattcctat atccggtcgc
     3001 gcaataggaa aattgtcacg ccacctcaac cccttggttc gggctaggcc gcagtcgctg
     3061 acaggatccc gggacgaagc cggagcgcgc gtcgaacgtc aggcggcgcg gatgccgctg
     3121 ctggtgtcgc catggacgcc gacgaccgcg tcatagccga ggatcatgtc gacgtttcgc
     3181 ccgtcgctgg ctagcggcag cctcagccac agcagcgaca tgtggccggc cccgcgccag
     3241 gccaggctgt ggcagccgac gccaggccga cgcccctcga ccaccttgtc gagttccgcg
     3301 cgccagtagt ccgcggcctt ggccccatag acatcgccaa gcgaccgccc ggtgatttcc
     3361 cggccataga cgctgtagag ccccgttccc gccagtcgca gccggtaatc cctgcgcggc
     3421 ccttcaacga tgtcgatcag gctgaccgtg ggcagcaggc gcttgaaatc ctcgggacgg
     3481 atgtccgccc ggccgggaag acggccggcc tggcgcaacg aggcccagta agcgaacaac
     3541 tcttcatggg cgcgggcggc tgtcgccgaa gcgcccagtt gcgcggccat ctacagaatc
     3601 cttaacaacc ccttggaatc accggtgatt cactatcgct taaccggcga atcgggggaa
     3661 gcgaaaatcg cgcgtcgcgt caaagtttcg cggccggagt caagccggcc cgcccgcggg
     3721 ccgccggcgc aaacaaaagg ggcggcgagt ccggcccgcc gcccctgatg ccctgacgcc
     3781 cggagggcgc ggctagcgct tcttcttcgc cgcctggcgg ccgccctgcc ccaggcccat
     3841 ctgcttggcc agatccgacc gcgccttggc gtagttggga gcgaccatcg gatagtcctt
     3901 cggcaggccc cacttggcgc ggtattcctc ggggctcatg ttgtatttgg tgcgcaggtg
     3961 acgcttgagc gacttgaact tgcgaccatc ctccagacag acgagatgat cgggggtgat
     4021 cgagcgacgc accggcacgg ccggctcctt cggcgcgacc tcgaccgtca ccgaaccgct
     4081 ggcgacatcg gtcagcgcct tgtgaacgtt ctggatcagg cccggaatat cattcgcggc
     4141 cacggaattg ttgccgacgt atgcggacac gatgtccgtc gtcatctcga tgatcgcgga
     4201 tttgtcttcc atctgaccta gttccatcac agtgaagatt gggcgcgggg ggaatcgacg
     4261 cgcttcgatt caattcatcg ctgcatatct cgccctacgg gcgtgcacaa gttcttgcga
     4321 gcaagaaatc gacccacttt cggtcaggct tcgcaattga aacagcaaca cgatcaatca
     4381 acgagagcgg cttcactctc gcgtttctga ccggcgacag gaaagtcacc gacgacgcag
     4441 atcgccggcc agggccagca tggcggcccg ctggggttgc gaaaactcgt ccagcgcctg
     4501 ctcgtcaccc tcgcggatgg cctggatcag ggccggggtc agggccgagg acaacgacca
     4561 gttccagtag cgcagaaccg tctgggcgcg atcgacggcg atggtgtcga acagggtcag
     4621 cacccgctcg agcgccggcg ccaggttgcc ggccgagtcg gtctccggtc gccgcagggc
     4681 gcgccacaga agccgcaggt tgttgcgcgg caggccggtc gccttgcaca ggatggccag
     4741 cggctcgccg cccggatcgg tgaagatctt ggccccggtc atcggcttga ggccggacag
     4801 gtaggagatc tcctcggcca cctcgcgggt catgctttcg gaggcgacca tgaccgcctc
     4861 gtcgaggctt tcgaacggac tcttggcgat ggccgcgcgg ttgcgctggc gccgctcgat
     4921 gaactgcagc gccttgcggg tcagggggtc ctgccagccc tcgtccgccg ccatggcgaa
     4981 gacatcgctg accgcctcct gcagaacctc ccgcgatacg gcgaaacgct ggaggatgat
     5041 ccggcgggct tcggcgtccg accaccagaa catcacatag gcgtggctgg gccgcagttc
     5101 gccccggcgc agcagcagcg gaatgtggcg cgggttgtcg cgcgtgcccg cgacgatggt
     5161 ctcgatcgcc tcgtggctga ggcgggcgag gtcattgcgc agcagggtct cgaccaccgc
     5221 cggctcgccc tgccggacca gttcgtcggc gaccacctcg ttgagaccgc gccgggcggc
     5281 gatcagccgg cgatgttcga ccgtcgcgct gcggacgcag tcgatcaggt cgccgtcgcc
     5341 gacggtcgcg ctgttctcga gcagcgccct cgccacttcg acgtcgtcac gcatcaacag
     5401 ccgggcgagg ctggtgggaa tttcggagac gttggcgata cgccgcgcga tgcgcacgcg
     5461 ctcgtcgatc gccgcctcgc gcagcagctc gatcagcagg tcggcggtca tcgcccgctc
     5521 gaaggcgttg acccggctgc ccggcaggca gaccacgtcg gccagtcgct tcagcagggt
     5581 cgcccgcgac cgcgccggcg cgggggccgg cgcagggtcc
//
LOCUS       DAORNX010000164         5596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10716_length_5596_cov_7.830536, whole genome shotgun sequence.
ACCESSION   DAORNX010000164 DAORNX010000000
VERSION     DAORNX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10716_length_5596_cov_7.830536"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="PLO65_08495"
     CDS             <1..1628
                     /locus_tag="PLO65_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRD28325.1"
                     /translation="LGGGGVVGYVDADTGAHVWRAIPFAAPPVGDLRWRAPRPPAAWT
                     GSRTAVTAAPWCPQQLSALDGVDRARFGEIVGQEDCLYLDVYAPPMDAVAAGAARLPV
                     MMWIHGGSNTWGRAEQYDGSALAARFKVVVVVVQYRLGALGWMANAALREGGTLPEDA
                     SPNFGTLDQIRALEWIRQDIGAFGGDPGRVTIFGESAGGQNVAALLASPRARGLFQRA
                     IIQSGSFRSEPMAEAEGLTGDRPESGRAVARRIVGPGQAATGEALRKAPLKAVFAAYR
                     LPDGEHDPMRVIADGVVLPAGGIASVLDQPDAFNAVPVITGTNQDEMKLFNALNPTLV
                     TWVFGRLPRPRDPAYFAAVSRYPSRMWRANAVDGPAARMTEGGHAPVWAYRFDWDEEG
                     RVLVTDLGQLLGSGHSLEIPFVFGRFKLLGAFDRYAFTKGNAAGRETLSDAMMSYWVN
                     FAASGAPGRGVDGRLPEWTAWSSAADANRLMLFDSQAGGGVRMSADHESGERIVADLI
                     ADTTIRTTTQRCEILARVRRDNPELQAAVACPP"
     gene            complement(1651..5043)
                     /locus_tag="PLO65_08500"
     CDS             complement(1651..5043)
                     /locus_tag="PLO65_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HRD28326.1"
                     /translation="MARFRGQATSDWLAVAPTGPAAVIGSGGDVAPDYFLNADQRGGI
                     ESSTGKVSKTVAQAASQLSRSFASWNDFHGEDQYVGPASTVTFAFRSTAPGAMPDDTG
                     GFSRFNTAQINATLNALQSWSDVANIIFVRAGSGTAGEEAYSDEATILFGNYATGASG
                     AAAFAYFPSLGASGGDVWVNSTLSYNANPVAGNYGQLVLIHELGHAIGFEHPGDYDAG
                     EGVDITYAANAEYFEDSHQYTVMSYFTENFTGGNFGPSYPATVMLDDIAVAQKVYGAN
                     MSTRTGDTVYGFNSNAGRDWFTASSASTILVFAVWDAGGTDTFDFSGYGNTQTINLNQ
                     GAFSSVGTYSGGNLVGNVAIAMGAVIENAIGGTGVDTIIGNAAANRLTGGAGDDNLTG
                     GAGADVFVASFGGGADTIEDFTAGSDRIDVTAFGAYQSIVQSGADTIVTLSGGVSMIL
                     RSVTASSVTNASFIGLTGPSDPLDGYNRIDGTAAGETLDGTTASDQIYGGDGADTLNG
                     LGMPDWLEGGAGGDVLNGGDGIDTASYAGSGTGVYVRLFNNTAFGGDAGGDVLSSIEN
                     ASGSSFNDDLRGSSVANVLDGAAGDDYLEGQGGNDTLLGGLGADTLVGGAGGDTLNGG
                     DGIDTISYVGSSLAVYVRLFNNLTFGGDAQGDVISGFENIIGSSNNDDLRGGNDVNLL
                     DGGAGNDLLMGFAGADTLIGGAGADTLNGGDGIDTVSYVGSSLAVYVRLFNNLTFGGD
                     AQGDVISGFENIIGSSNNDDLRGGNDVNLLDGGAGNDLLMGFAGADTLIGGAGADTLD
                     GGDGLDTISYVGSAVGVFVRLFNNLTFGGDAQGDVISGFENIIGSSNNDDLRGGNDIN
                     VLDGGAGNDILMGFAGADTLIGGAGGDTLTGGDGLDTISYVGSSLAVYVRLFNNLTFG
                     GDAQGDVISGFENIIGSSNNDDLRGGNDVNLLDGGAGNDLLMGFAGADTLTGGLGNDS
                     LAGGAGADRFVFTGASGSDTITDFAAGVGAGDVIQLSTAIFTSFADVLANTADDGLGN
                     TVISKGGVTIKLTGVLESQLAADDFAFVAGALPAAPTAPASAPSSFDAMPALDSKAGV
                     DLWSAATIQDAQDGAEPGLGLGGPGFERPHLFAAPSDYDWVW"
     gene            5209..>5596
                     /locus_tag="PLO65_08505"
     CDS             5209..>5596
                     /locus_tag="PLO65_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRD28327.1"
                     /translation="MSPDLARLLLNLGLATIMMAITVLVHFWGLVVLVRLMGHTGRRL
                     RAHENSIGQAALLVFVVFGLIGLHTIEIWLYAIVYMELGALPTLESALYFSTVTFVTV
                     GYGDIVLGPAWRLLSAIEAANGVILVA"
BASE COUNT          829 a         1954 c         1884 g          929 t
ORIGIN      
        1 tcctcggcgg cggcggcgtg gtcggctatg tcgacgccga caccggcgcc catgtctggc
       61 gggccatccc gttcgccgcg ccacccgtgg gtgatctgcg ctggcgcgcg ccgcgaccgc
      121 ccgccgcctg gacaggatcg cggaccgccg tcaccgccgc accctggtgt ccgcaacagc
      181 tcagcgccct agacggcgtc gacagggcgc gcttcggcga gatcgtcggc caggaagact
      241 gcctctatct cgacgtctac gccccaccga tggacgccgt tgcggccggc gccgcgcgcc
      301 tgccggtgat gatgtggatc catggcggat cgaacacctg gggccgggcc gaacagtatg
      361 acggatctgc cctggccgcc cggttcaagg tcgtggtggt ggtcgtgcag tatcgcctcg
      421 gcgcccttgg gtggatggcc aacgccgcct tgcgggaagg cggaaccctg cccgaggacg
      481 cctcgccgaa cttcggcacg ctcgaccaga tccgcgccct cgagtggatt cgccaggaca
      541 tcggcgcctt tggcggcgat ccgggccggg tgaccatctt tggcgaaagc gccggcggcc
      601 agaacgtcgc ggccctgctc gccagccccc gggctcgcgg cctgttccag agggccatca
      661 tccagtccgg ctcgttccgt tcggaaccca tggccgaggc cgagggcctg acaggcgacc
      721 gcccggaatc gggccgcgcc gtggccaggc ggatcgtcgg tccgggccag gccgcgaccg
      781 gcgaagccct gcgcaaggcc ccgctcaagg ccgtcttcgc cgcctaccga ctgccggacg
      841 gcgaacatga tcccatgaga gtcatcgccg acggcgtggt gctgcccgcc ggcggcatcg
      901 cgtccgtgct ggaccagccc gacgccttca acgccgtgcc ggtgatcacc gggacaaacc
      961 aggacgagat gaagctgttc aatgcgctca atcccaccct ggtgacctgg gtgttcggac
     1021 ggctgccccg gcctcgcgat ccggcctact tcgcggcggt gtcccgctat ccgagccgga
     1081 tgtggcgggc caacgccgtc gacggtccgg cggcccgcat gacggagggg ggccacgccc
     1141 cggtgtgggc ctaccggttc gactgggacg aggaaggacg ggtgctcgtc accgatctgg
     1201 gtcagttgct cggctcaggc cacagcctcg agattccgtt cgtgttcgga cgcttcaagc
     1261 tgctgggcgc cttcgatcgc tacgccttca ccaagggcaa tgcggccggt cgcgagacgc
     1321 tgtccgacgc aatgatgagc tactgggtca atttcgccgc gtccggcgcg cctggccgcg
     1381 gcgtcgacgg ccgcctgccg gaatggacgg cctggtcgag cgccgcggac gccaaccgcc
     1441 tgatgctgtt cgacagccag gccggcggcg gcgtgcggat gagcgctgac cacgagtccg
     1501 gcgagcgcat cgtcgccgac ctgatcgccg acacgacgat cagaacgacg acgcagcgtt
     1561 gcgaaattct cgcccgggtg cgccgcgaca accccgaact gcaggccgcc gtcgcctgtc
     1621 ctccgtaaga cggcggcgac ccgcgcgggg ctaccagacc cagtcgtagt ccgagggcgc
     1681 agcgaacagg tgcggccgtt cgaagcccgg accgccgagg cccagaccag gctcggcgcc
     1741 gtcttgcgcg tcctggattg tcgcggcgct ccacaggtcg acgccggcct tggagtcaag
     1801 cgccggcatg gcgtcgaagg acgacggcgc cgaggccggc gccgtcggcg cggccggcag
     1861 ggcgccggcg acgaaggcga agtcgtcggc ggccagctgg ctttcgagca ccccggtcag
     1921 cttgatcgtc accccgccct tgctgatcac cgtgttgccc aggccgtcat cggcggtgtt
     1981 ggccagcacg tcggcgaagc tggtgaagat ggccgtgctg agctggatca cgtcccccgc
     2041 cccgaccccg gcggcgaagt cggtgatggt gtcactgccc gaggcgccgg tgaacacgaa
     2101 ccggtcggcg ccggccccgc cggccagact gtcgttgcca aggcctccgg tgagggtgtc
     2161 ggccccggca aagcccatca gcaggtcgtt gcccgcgccg ccgtccagca ggttgacgtc
     2221 gttgccgccc cgcaggtcgt cgttgttcga tgagccgatg atgttctcga agccgctgat
     2281 cacatcgccc tgggcgtctc cgccaaaggt caggttgttg aacagccgca catagaccgc
     2341 cagcgaggag ccgacgtagc tgatggtgtc caggccatcg ccgccggtca gggtgtcgcc
     2401 gcccgccccg ccgatcagcg tatcggctcc ggcgaagccc atcagaatgt cgttgcccgc
     2461 cccgccatcc agcacattga tgtcgttgcc gccccgcagg tcgtcgttgt tcgacgagcc
     2521 gatgatgttc tcgaagccgc tgatcacatc gccctgggcg tcaccgccaa aggtcaggtt
     2581 gttgaacagc cgcacaaaca cccccaccgc cgatccgacg tagctgatcg tgtcgagacc
     2641 atcaccgccg tccagcgtgt cggctccggc cccgccgatc agggtgtcgg cgccggcaaa
     2701 gcccatcagc aggtcgttgc ccgcgccgcc gtccagcagg ttgacgtcgt tgccgccccg
     2761 caggtcgtcg ttgttcgatg agccgatgat gttctcgaag ccgctgatca catcgccctg
     2821 ggcgtctccg ccaaaggtca ggttgttgaa cagccgcaca tagaccgcca gcgaggagcc
     2881 gacgtagctg acggtgtcga tcccgtcgcc cccgttcagc gtgtcggctc cggccccacc
     2941 gatcagggtg tcggcgccgg caaagcccat cagcaggtcg ttgcccgcgc cgccgtccag
     3001 caggttgacg tcgttgccgc cccgcaggtc gtcgttgttc gatgagccga tgatgttctc
     3061 gaagccgctg atcacatcgc cctgggcgtc tccgccaaag gtcaggttgt tgaacagccg
     3121 cacatagacc gccagcgagg agccgacgta gctgatggtg tcgatcccgt cgccgccgtt
     3181 cagcgtgtcg ccgcccgcgc cgccgaccag ggtgtcggcg ccaaggccgc ccagcagggt
     3241 gtcattgccg ccctgcccct cgaggtagtc gtcgcccgcg gcgccatcca gcacattggc
     3301 gacggacgag ccgcgcagat cgtcgttgaa ggacgagccg gacgcgttct cgatgctcga
     3361 caggacatcg ccgccggcgt cgccgccgaa ggccgtgttg ttgaacaacc gcacatagac
     3421 cccggtcccg gagccggcat agctggccgt gtcgatcccg tcgccgccgt tcaggacatc
     3481 gccgccggcc ccgccttcca gccagtcggg catacccagg ccgttgagcg tgtccgcgcc
     3541 gtcgccgccg tagatctggt cgctggccgt ggtgccgtcg agcgtctcgc cggccgcggt
     3601 gccgtcgatg cggttgtagc cgtcgagcgg atctgaaggg cccgtcaggc cgatgaagct
     3661 ggcgttggtc acggacgagg ccgtgaccga tcgcaggatc atgctcacgc cgcccgacag
     3721 ggtgacgatc gtgtcggccc cgctctggac gatgctctgg taggccccga aggcggtcac
     3781 atcgatcctg tcggagcccg ccgtaaagtc ttcgatggtg tcggcgccgc cgccgaacga
     3841 agcgacaaat acatcggccc ccgccccgcc ggtcaggttg tcgtcgccgg ccccgccggt
     3901 caggcggttg gccgcggcgt tgccgatgat ggtgtcaacg ccggtcccgc cgatcgcgtt
     3961 ctcgatcacc gcgcccatgg cgatggcgac attgcctaca aggttgccgc ccgaataggt
     4021 cccgacgctc gagaaggctc cctggttgag attgatcgtc tgggtgttgc cgtagcccga
     4081 gaagtcgaag gtatcggtcc caccggcgtc ccacaccgcg aacaccagga tggtgctcgc
     4141 cgaactggcc gtgaaccagt cgcgcccggc gttcgaattg aagccgtaga ccgtgtcgcc
     4201 ggtccgggtg gacatgttgg ccccgtagac cttctgggcg acggcgatgt catccagcat
     4261 gacggtcgcc ggatagctcg gcccgaagtt cccgccagtg aaattctcgg tgaagtaact
     4321 catcaccgtg tactggtgac tgtcctcaaa atattcggcg ttggccgcgt aggtgatgtc
     4381 gacgccctct ccggcgtcat agtcgcccgg gtgctcgaag ccgatcgcgt gacccagctc
     4441 gtgaatcaac accagctgcc cgtagttccc ggccacggga ttggcgttgt agctgagggt
     4501 gctgttgacc cacacgtcac cgccgctcgc gccgaggctg gggaagtagg cgaacgcggc
     4561 cgctccggac gctccggtgg cgtagtttcc gaacaggatg gtcgcctcgt cggaataggc
     4621 ctcttcaccc gccgtacccg atccggcccg gacaaagatg atattggcca cgtccgacca
     4681 ggactgcagg gcgttgagcg tcgcattgat ctgcgcggtg ttgaagcggc tgaacccgcc
     4741 cgtgtcgtcg ggcatggcgc ccggcgcggt cgaccggaag gcgaaggtca ccgtcgaagc
     4801 cggcccgacg tactggtcct cgccgtgaaa gtcgttccat gaggcgaacg agcggctgag
     4861 ctgcgacgcg gcctgggcga ctgtcttgga caccttgccg gtggaggatt cgatcccgcc
     4921 gcgctggtcg gcgttgagga agtagtccgg tgcgacgtct ccgcccgacc cgatcaccgc
     4981 cgccggcccg gtcggcgcga ccgcgagcca gtccgacgtc gcctgcccac gaaacctggc
     5041 catccccgta cgctccgctg cctactcacc gcgaccggac ggtaggcttt cggagggcgt
     5101 cggacaagcg ggcggtccgc cgcaggcggc cccgcgcggc gtggtcgcag gcccgtcgtg
     5161 cgattagggc gttccgcgcc gccatcgccg gtgtagggtc tggcggtcat gtcgcccgac
     5221 cttgcacgcc tgcttctgaa cctgggtctg gccaccatca tgatggccat caccgtgctg
     5281 gtgcacttct ggggcctggt cgtgctggtc cggctcatgg gccacaccgg ccgccggttg
     5341 cgggcgcacg agaactccat cggccaggcg gcgctgctgg tgttcgtagt gttcggcctg
     5401 atcggcctgc acaccatcga gatctggctc tacgccatcg tctacatgga gctcggggcc
     5461 ctgccgaccc tggaatcggc gctctatttt tcgaccgtga cctttgtcac cgtgggctac
     5521 ggcgacattg tcctcgggcc ggcctggcgg ctgctgagcg cgatcgaggc ggccaacggc
     5581 gtgatcctcg tcgcct
//
LOCUS       DAORNX010000165         5583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10747_length_5583_cov_7.382959, whole genome shotgun sequence.
ACCESSION   DAORNX010000165 DAORNX010000000
VERSION     DAORNX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10747_length_5583_cov_7.382959"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLO65_08510"
     CDS             <1..273
                     /locus_tag="PLO65_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28328.1"
                     /translation="IERDQADPQPRFGLGLALYFSSQGAGRILGPLLAGTARLAVVGV
                     GGLLLVRMQAPSWALFALVAAGMVTMGAVTAASVALTPWGPRLSRT"
     gene            complement(260..2056)
                     /locus_tag="PLO65_08515"
     CDS             complement(260..2056)
                     /locus_tag="PLO65_08515"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD28329.1"
                     /translation="MADDLGFDPQALREKYRIERDRRLREDANEQYREIKGDFAHYLE
                     DPYVEPGFTREPLTDEIEVVVIGGGFGGLLAGARLREAGVQDIRMIEKGGDFGGTWYW
                     NRYPGAACDVESYIYLPLLEELGFMPVEKYTRAPEILAHCRAIGEHFDLYRDVCFQTE
                     ITDMSWDAASSRWIIRTNRGDAMKAKFVIMANGPLHRPKLPGIPGVETFKGHSFHTSR
                     WDYAYTGGDSFGNLTGLKDKRVGVIGTGATAVQCVPHLGASAKELYVFQRTPSSIDVR
                     DNRPTDPAWAASLEPGWQQRRMDNFNNLVSGGFEKEDLVGDGWTDIIRNILLIGRRNR
                     EAGEAPMELQELMQLADFKKMEQIRARVDEIVQDPATAEALKPWYNQFCKRPCFHDDY
                     LPTFNRPNVTLVDTQGRGVERITENAVWVDGKAYEVDCLVYATGFEVGTSYARRAGYE
                     VHGRDGLTLTDKWKDGVATLHGMHVHGFPNLFIFSNSQSGFSVNYPHMLNAQAQHASW
                     IIAEAMKRQASTIEATRAAEDAWVQTIIDSALMRQKFQEECTPGYYNNEGRPSALAAR
                     NGPYGKGSIAFIKLMEDWRAAGALEGLELTSG"
     gene            complement(2121..3734)
                     /locus_tag="PLO65_08520"
     CDS             complement(2121..3734)
                     /locus_tag="PLO65_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD28330.1"
                     /translation="MAETFDYIIVGAGSAGCVLANRLSADPKTRVLVLEAGGRDDWIW
                     FHIPVGYLFSIGNPRADWMFETQAIPGLNGRSLKYPRGKVIGGSSAINAMIYMRGQAA
                     DYDGWRQLGLTGWGWDDVLPLFLAQEDHIAPPSEHHRSGGEWRVEHPRMRWTVLDAFA
                     EAAALEGIAPVADFNGGDNAGSGYFQVNQRAGVRWSAARGFLKPALRRSNLKLETGAR
                     VDRVLIENGRAVGVAWSRDGQAMEARAEGEVILSAGTVATPLLLERSGLGDGERLRSL
                     GVEPVRHLPGVGENLQDHLQIRPIYQVSGVPTMNALYASLWRRPLMALEYAALRRGPM
                     SMAPSQMGAFAKSSPDQVTANLQFHVQPLSLDRFGDPLHPFPAITISVCNLRPTSRGS
                     IHAGGPGADAPPVIQPNYLDTAEDGRVAVDSMRLVRRIVGQPPLARYGPREMKPGPAV
                     DDDAGLLAAARDLGTTIFHPVGTARMGLDSDPMAVVDERLRVTGVAGLRVADASVMPR
                     ITSGNTNSPTMMIAEKAARMIVEDRRQPS"
     gene            3890..5176
                     /locus_tag="PLO65_08525"
     CDS             3890..5176
                     /locus_tag="PLO65_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HRD28331.1"
                     /translation="MDRRSMLWGAAGAGLLAGSWPAMAAGQTGRLRRPVSSLSPNDPD
                     IEALRQAIPLLRRSGAWDAQVALHADMRHRHHSSWRFLPWHRLQLVWFERHVARASGK
                     ADFALPYWDWDDDRIPDLFFDDDVFYVPGREARPDETISEFMRRNGQRFTGRLSDDFA
                     TFFGRPRGLADPTDGDLGRRYYSGSGEWSGHNLIHGFVGGDMGRLDRSPNDPVFWMHH
                     ANIDRIWAAWQDRHSGQTYPRAWRDEMLGGFLDPDGRMVPPTPAAGTLDTATFGYRYQ
                     IDPTPPIVFAPAPGQPFKRQRRSTYSWSMQRMGPGSAFVEISRSLARGRAESATGYLE
                     LIPDPHAASMVTLTARDLSDGSEVFRDAVFLVPMGHAMGSQNYRMRMDSLWSGGDPAG
                     TVRLEIEAAPLVGRRAGDMPTTLVEFVLDADLVFTD"
     gene            5285..>5583
                     /locus_tag="PLO65_08530"
     CDS             5285..>5583
                     /locus_tag="PLO65_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28332.1"
                     /translation="MISLLLALAGAAAPQEGQATLQSCEMTASGWVCHYQLPPVTLIG
                     TPDKEPPTSVAPPTVTVTPPPVAPDPAGELERAEAARQAKLIANCADATWLSLCLP"
BASE COUNT          783 a         1968 c         1923 g          909 t
ORIGIN      
        1 atcgagcgcg accaggccga cccgcagccg cgcttcggcc tcggcctggc gctgtatttc
       61 tcttcgcagg gcgccggccg gatcctcggc cctcttctgg ccggcacggc ccgcctggcc
      121 gtggtcgggg tgggcgggct gttgctggtg cggatgcagg cgccgtcctg ggccctgttc
      181 gcgctggtgg cggcgggcat ggtcaccatg ggcgccgtga ccgccgcctc cgtggccttg
      241 acgccctggg gcccgcgcct cagccggacg tgagttccag cccctccagc gcgccggccg
      301 cgcgccagtc ctccatcagc ttgatgaagg cgatcgagcc cttgccatag gggccgttgc
      361 gggccgccag ggccgagggc ctgccctcgt tgttgtagta gcccggcgtg cattcctcct
      421 ggaacttctg ccgcatcagg gccgagtcga tgatcgtctg cacccaggcg tcctcggccg
      481 cccgggtggc ctcgatggtg ctggcctggc gcttcatggc ctcggcgatg atccacgagg
      541 catgctgcgc ctgggcgttg agcatgtgcg gatagttgac cgagaagccc gactggctgt
      601 tgctgaagat gaacaggttg gggaagccgt gcacgtgcat gccgtgcagg gtcgcgaccc
      661 cgtccttcca cttgtccgtc agggtcaggc cgtcgcggcc atgcacctcg tagcccgcgc
      721 gccgggcgta gctggtgccg acctcgaagc cggtggcgta gaccaggcag tcgacctcgt
      781 aggccttgcc gtcgacccac acggcgttct cggtgattcg ctcgacgccg cgcccctggg
      841 tgtcgaccag cgtcacgttg ggccggttga aggtcggcag gtagtcgtcg tggaagcagg
      901 gccgcttgca gaactggttg taccagggct tcagcgcctc ggccgtggcc gggtcctgga
      961 cgatctcgtc cacccgggcg cggatctgct ccatcttctt gaaatcggcc agctgcatca
     1021 gttcctgcag ctccatcggc gcctctccgg cctcgcggtt gcggcgaccg atcagcagga
     1081 tgttgcggat gatgtcggtc cagccgtcgc cgaccaggtc ctccttctcg aacccgcccg
     1141 agacgaggtt gttgaagttg tccatccggc gctgctgcca gcccggctcc agcgacgcgg
     1201 cccaggccgg gtcggtcggc cggttgtcgc gcacgtcgat cgacgagggc gtgcgctgga
     1261 agacgtagag ttccttcgcc gacgccccca ggtgcggcac gcactgcacg gccgtggcgc
     1321 cggtgccgat gaccccgacc cgcttgtcct tcaggccggt caggttcccg aacgagtcgc
     1381 cgccggtgta ggcgtagtcc cagcggctgg tgtggaagct gtgacccttg aaggtctcca
     1441 cgcccgggat gcccggcagc ttgggccggt gcagcgggcc gttggccatg atgacgaact
     1501 tcgccttcat ggcgtcgccg cggttggtgc ggatgatcca gcgcgaggac gccgcgtccc
     1561 agctcatgtc ggtgatttcg gtctggaagc agacgtcgcg gtagaggtcg aaatgctcgc
     1621 cgatggcccg gcagtgggcg aggatttccg gggcccgcgt atacttctcg accggcatga
     1681 agccgagttc ctcgagcagc ggcaggtaga tgtaggactc cacatcgcag gccgcgcccg
     1741 ggtagcggtt ccagtaccag gtgccgccga agtcgccgcc cttctcgatc atgcggatgt
     1801 cctggacgcc ggcctcgcgc agccgggccc cggccagcag gccgccgaag ccgccgccga
     1861 tgaccaccac ctcgatctcg tcggtcagcg gctcgcgcgt gaaaccgggc tcgacgtagg
     1921 ggtcctcgag gtagtgggcg aaatcgccct tgatctcgcg gtactgctcg ttggcgtcct
     1981 cgcgaagccg cctgtcgcgt tcgatgcggt acttttcgcg cagcgcctgc gggtcgaatc
     2041 cgagatcgtc ggccatctgt gtcctgccca ttccataccg gcggttcgaa aacagtcttc
     2101 accgcccgac gcgacggaaa tcaagagggc tgtcgtcgat cctcgacaat catccgcgca
     2161 gccttctcgg cgatcatcat cgtcggagag ttggtgttgc ccgaggtgat gcgcggcatg
     2221 accgaggcgt cggcgacgcg caggcccgcg acgccggtca cccgcaggcg ttcatcgacc
     2281 acggccatcg ggtcggaatc caaacccatc ctcgccgtgc cgaccggatg gaagatggtc
     2341 gtgccgaggt cccgggccgc cgccagcagg ccggcgtcgt cgtcgacggc cggacccggc
     2401 ttcatctccc gcgggccata gcgcgccagg ggcggctggc caacgatccg ccgcaccagt
     2461 cgcatcgagt cgacggccac ccggccgtcc tccgccgtgt cgaggtagtt gggctggatg
     2521 accggcggcg cgtccgcccc ggggccgccg gcatggatcg agccgcgact ggtcgggcgc
     2581 aggttgcaga cgctgatcgt gatcgccgga aacggatgca gcgggtcgcc gaagcggtcg
     2641 agcgacagcg gctggacatg gaactgcagg ttggccgtga cctggtcggg cgacgacttg
     2701 gcgaaggccc ccatctgcga cggcgccatc gacatcggcc cgcgccgcag ggcggcgtat
     2761 tccagcgcca tcagcggccg gcgccatagc gaggcgtaga gggcgttcat cgtcggcacg
     2821 ccgctgacct ggtagatcgg gcggatctgc aggtggtcct gcaggttctc gccgacgccg
     2881 ggcaggtggc ggacgggctc gacccccagc gatcgcaggc gttcgccatc acccaggccg
     2941 gaccgctcca gcagcaacgg cgtggccacg gtccccgcgg acaggatgac ctccccctcg
     3001 gcccgcgcct ccatcgcctg gccgtcgcgc gaccaggcca cgccgaccgc ccggccgttc
     3061 tcgatcagca cccggtcgac ccgcgcgccg gtctcgagct tgaggttcga tcgccgcagg
     3121 gccggcttga ggaagccgcg cgccgcgctc caccgcacgc cggcccgctg gttgacctgg
     3181 aaatagccgc tgcccgcgtt gtcgccgccg ttgaagtcgg ccaccggcgc gatgccttcc
     3241 agcgcggcgg cctcggcgaa ggcgtccagc acggtccagc gcatgcgcgg atgctcgacc
     3301 cgccactcgc cgccggaacg gtgatgctcc gacggcggcg cgatgtggtc ttcctgggcc
     3361 aggaacagcg gcagcacgtc gtcccagccc cagcccgtca ggcccagctg gcgccagccg
     3421 tcgtagtcgg ccgcctggcc gcgcatgtag atcatcgcgt tgatcgccga ggagccgccg
     3481 atcaccttgc cgcgcgggta cttcaggctg cggccgttca ggccggggat ggcctgggtc
     3541 tcgaacatcc agtcggcgcg cgggttgccg atcgagaaca ggtagccgac cggaatatgg
     3601 aaccagatcc agtcgtccct gccgccggcc tccagcacca gcacacgggt cttcggatcg
     3661 gccgacagcc ggttggccag cacgcagccg gccgacccgg cgccgacgat gatgtagtcg
     3721 aaggtctcgg ccattttcgt ccccacgggc ggacagatgc cacgattggc cctggcgcgc
     3781 atccgtcggc gggcaaggat ggccggtcag gcgatctcct cagacgcgat ggcgtttgac
     3841 atccgccgcc gcgactcggc gaatgatcca ggggggagcc tctatgacct tggatcgtcg
     3901 ttccatgctg tggggtgcgg ctggcgcggg cctgctggcg ggcagctggc cggccatggc
     3961 cgccggccag acgggccggc tgcgccggcc tgtgtcgagc ctctcgccca acgatcccga
     4021 catcgaggcg ctgcgccagg cgattccgct gctgcgacgg tcgggcgcct gggacgccca
     4081 ggtcgcgctc cacgccgaca tgcggcaccg gcaccactcc tcctggcggt tcctgccctg
     4141 gcatcgcctg cagctggtct ggttcgagcg ccatgtggcg cgcgccagcg gaaaggccga
     4201 tttcgccctg ccctactggg actgggacga cgatcgcatc ccggacctct ttttcgacga
     4261 tgatgtattt tacgtgcctg gccgcgaagc gcggcccgac gagaccatca gcgaattcat
     4321 gcgccgcaat ggccagaggt tcaccggccg gctgtcggac gacttcgcga ccttctttgg
     4381 ccgccccagg ggtctggccg acccgaccga cggcgacctt ggccggcgct actattcagg
     4441 gtcgggcgaa tggagcgggc ataacctgat ccacggcttc gtcggcggcg acatgggccg
     4501 tctcgatcgc tcgccgaacg atccggtctt ctggatgcac cacgccaaca tcgaccggat
     4561 ctgggcggcg tggcaggacc gccattccgg ccagacctat ccccgggcct ggcgcgacga
     4621 aatgctgggc ggattcctcg accccgacgg gcgaatggtt ccccccacgc cggccgccgg
     4681 cacgctggac accgccacgt tcggctatcg ctaccagatc gatccgacgc cgccgatcgt
     4741 cttcgccccg gcgcccggac agccattcaa gcgccaacgc cgaagcacct acagctggtc
     4801 catgcagcgg atggggcccg ggtcggcctt cgtcgagatc tcgcgcagcc ttgcccgcgg
     4861 tcgggccgaa agcgccaccg gctacctgga gctgatcccc gacccgcacg ccgcttccat
     4921 ggtgaccctg acggcgcggg acctgtcgga cggatcggag gtcttccgcg acgccgtgtt
     4981 cctcgtaccc atgggccacg ccatggggtc gcagaactat cgcatgcgga tggactcgct
     5041 gtggagcggc ggggatccgg ccgggaccgt gcggctggag atcgaggcgg cgccgctggt
     5101 cggccgccgc gccggcgaca tgccgaccac cctcgtggaa ttcgtcctcg acgcggacct
     5161 ggtgttcacc gactgaccgc cttcgcgccg gcgaggcggc gcgtggggtg taaacgtttg
     5221 ggccgctccc caatcggcga tatggtcgcc gtggcgaggg gatcgcaccg tcagaggttg
     5281 agccatgatt tcgctgttgc tggccctggc cggagccgcg gcgccccagg aggggcaggc
     5341 cacgctgcaa tcctgtgaaa tgaccgcgag tggctgggtc tgccactatc agctgccccc
     5401 ggtcaccctg atcgggactc ccgacaagga gcccccgacg agcgtcgcgc cccccaccgt
     5461 gaccgtgacg ccacccccgg tcgcgcccga tccggccggc gagctcgagc gggccgaggc
     5521 cgcgcgccag gcgaagctga tcgccaactg cgccgacgcg acctggctgt cgctgtgcct
     5581 gcc
//
LOCUS       DAORNX010000166         5581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10757_length_5581_cov_6.744662, whole genome shotgun sequence.
ACCESSION   DAORNX010000166 DAORNX010000000
VERSION     DAORNX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10757_length_5581_cov_6.744662"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1077)
                     /locus_tag="PLO65_08535"
     CDS             complement(169..1077)
                     /locus_tag="PLO65_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD28333.1"
                     /translation="MSAADMIPGSPTRAALLNNAVKGRSLWDDARRRLLRNKAAVTGM
                     AVLAAMVLVAVIGPALAPFHYDTVTKTDVWMAPMTGGHLLGTDSLGRDLLARLLAGLG
                     VSLAIGVVATSVSLVIGVLWGAVAGYLGGRIDEIMMRVVDTLYSLPFIFFVILLMVTF
                     GRNIFLIFLAIGAVEWLTMSRIVRGQTLSLKSKEFIEAARAAGLSSGQIIARHIVPNL
                     LGPVVVYVTLTIPAVIIAESFLSFLGLGVQPPMASLGTLIATGAQDMEMAPWLLIFPS
                     VTMVVTLMAFNFIGDGLRDALDPKDR"
     gene            complement(1074..1997)
                     /gene="oppB"
                     /locus_tag="PLO65_08540"
     CDS             complement(1074..1997)
                     /gene="oppB"
                     /locus_tag="PLO65_08540"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HRD28334.1"
                     /translation="MLRFLFRRLLVAIPTLLLVITVAFFMMRAAPGSPLVNERQLSPE
                     VEKAVMAKFGMDRPLTEQYVNYLKGVAVGDFGPSLKYRDKTVLEILQDNYRVSLTLGG
                     LSILLAALVGVSLGVIAALRQNRAADYVTMAVAVLGVCIPTFVTAPLLVLVFASKLGW
                     LPTAGWNDGALPNLVLPIVVLALPQIAIISRLTRAGMIEVLRSNYIRTARAKGLPESL
                     IVRKHALRAAIMPLVSYLGPAAAGLLTGSLVVEKIFNLPGLGKFFVNSALQRDYTVVM
                     GMVIVYAVLILVLNLIADVLYAALDPRVRLS"
     gene            2093..2500
                     /locus_tag="PLO65_08545"
     CDS             2093..2500
                     /locus_tag="PLO65_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HRD28335.1"
                     /translation="MTEFRRVTDDFSVSPQITPEAMAAAAAQGFTLVINNRPDGEVPD
                     QPTSAQMEAAARAAGMDYVHNPVTGSPTQDQVEAQRLAIEDADGPVLAFCRSGTRSIV
                     TWSLGQALSGARSRSELAALGRQAGYDLSGVLG"
     gene            complement(2529..2678)
                     /locus_tag="PLO65_08550"
     CDS             complement(2529..2678)
                     /locus_tag="PLO65_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28336.1"
                     /translation="MAADAAIFVSKRLQRAAFAFLCLVTAQLVLASCEPEPSAMAARV
                     TLGGV"
     gene            2831..3760
                     /locus_tag="PLO65_08555"
     CDS             2831..3760
                     /locus_tag="PLO65_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28337.1"
                     /translation="MAGTLWTSDFHFESGRVKVRTTGADLALDRALLGDVGLWLAYYL
                     PVRLKGLVTAVARPGPRIWFAPDAPRPWYLLGLAACWAGVRVASSPDKADAAVYFEDV
                     TVAAPPACDLPRRFNFACGDVSKSRVAAVFEQVFGYPLAVDPTTWRGLAVEKGEGNGA
                     HDGRIVQCPCPAQPGRVYQRVVDTVEDDHVVDLRTPCVGGAPVLTFIKRRPARDRFAN
                     YNASVSLHRPDELFTPQELAVIERFCAAMNLDWGGLDILRDRADGRLYIVDVNKTDMP
                     PLRLPWRQKMEAIARLAKAFLVMVDGATARGSA"
     gene            3757..4650
                     /locus_tag="PLO65_08560"
     CDS             3757..4650
                     /locus_tag="PLO65_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD28338.1"
                     /translation="MIPFVRDIRFDYGACDRLTPLIRRVVANNPGPFTFTGTGTYIIG
                     NGEVAVIDPGPDMAEHLDAILAATAGERISHIFVTHNHMDHSPLARPLAAVTGATIYA
                     CAMQGVATESEVRLEAGDDRVFTPDVGLCDGGVFKGGDWTLEAIPTPGHTSNHICYAL
                     IEENALFSGDHIMGWSTTVISPPDGDMGDYFASLERVKARDFATIWPTHGPPITATGP
                     FVQAYIDHRRAREAQILAALGSGHTRIKPMVADLYREVDARLHPAAAHSVMAHMIQLV
                     KEGRVVCDGPPGLDSDYRLAG"
     gene            complement(4777..>5226)
                     /locus_tag="PLO65_08565"
     CDS             complement(4777..>5226)
                     /locus_tag="PLO65_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRD28339.1"
                     /translation="RASRWKKKKTAPPAALKARGPDANPVDPSPVLPLGSQAYAGRRV
                     AEVNAETCPAARPVTVAISAAQAFAHARSAVERAGMSIITADAASGRIEATAVSLLYG
                     FKDDVVVRVRPAGAGARIDLRAVRRTGDSDLGANCRRIGTLVTAIAG"
     assembly_gap    5227..5331
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5332..>5581)
                     /locus_tag="PLO65_08570"
     CDS             complement(<5332..>5581)
                     /locus_tag="PLO65_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28340.1"
                     /translation="KAAGGGWAGRYLDFALLVALAPAAMWLFGALAAKFNLITPQQGF
                     EAMLVQGPTESLGWAPAIALVGVLTGLVGVLVAVFGGFS"
BASE COUNT          887 a         1929 c         1915 g          745 t
ORIGIN      
        1 gcggcacggc tcgacggtcg agattcgcac cgcgaccatg ggcagcttct tcatgaaggt
       61 cgatggccag ccgcagatcc gcgtgcaccg cgacaactga ggccgacggc cggcgggccg
      121 ggccggttcg ccgggccgcc cccggccggg tctggcgtcg cgcggccact agcggtcctt
      181 ggggtcgagc gcgtcccgca ggccgtcgcc gatgaagttg aaggccatca gggtcacgac
      241 catcgtcacc gacgggaaga tcagcagcca gggggccatc tccatgtcct gggcgccggt
      301 ggcgatcagg gtgccaagcg aggccatcgg cggctgcacg ccgaggccga ggaacgacag
      361 gaagctctcg gcgatgatca ccgccggaat ggtcagggtg acatagacca cgaccggacc
      421 cagcaggttg ggcacgatgt ggcgcgcgat gatctggccc gacgacaggc ccgcggcgcg
      481 ggcggcctcg atgaattcct tgctcttcag cgacagggtc tggccgcgca cgatgcgcga
      541 catggtcagc cactcgaccg cgccgatggc caggaagatc aggaagatgt tccggccgaa
      601 cgtcaccatc agcaggatga cgaagaagat gaacggcagg gaatagaggg tgtcgaccac
      661 ccgcatcatg atctcgtcga tacggccgcc cagatagccg gccaccgcgc cccacagcac
      721 gccgatgacc agcgacacgc tggtggccac cacgccgatg gccagcgaca cgcccagccc
      781 ggccagcagg cgcgccagca ggtcccggcc cagcgagtcg gtccccagca ggtggccgcc
      841 ggtcatcggc gccatccaga cgtcggtctt ggtcacggtg tcgtagtgga acggcgccag
      901 ggccggcccg atcacggcca ccaggaccat cgccgcaagc accgccatgc ccgtgacggc
      961 cgccttgttg cgcagcagac ggcggcgggc gtcgtcccac agcgagcggc ccttgacggc
     1021 attgttcagc agggccgccc gggtcggcga gccggggatc atgtccgcgg cgctcatgac
     1081 agccgcaccc gcgggtcgag cgccgcgtag agcacgtcgg cgatcaggtt cagcaccagg
     1141 atcagcacgg cgtagacgat gaccatcccc atcaccacgg tgtagtcgcg ctgcagggcg
     1201 ctgttgacga agaacttgcc caggcccggc aggttgaaga tcttctcgac caccagcgag
     1261 ccggtcagca ggcccgcggc ggccgggccg aggtagctga ccagcggcat gatcgccgcg
     1321 cgcagggcgt gcttgcgcac gatcaggctc tccggcaggc ccttggcccg ggcggtgcgg
     1381 atgtagttgg agcgcagcac ctcgatcatg cccgcccggg tcaggcgcga gatgatggcg
     1441 atctgcggca gcgccagcac gacgatcggc agcaccaggt tgggcagggc cccgtcgttc
     1501 cagccggccg tcggcagcca gccgagcttc gaggcgaaca ccagcaccag cagcggggcg
     1561 gtgacgaagg tggggataca gacgcccagc accgccaccg ccatggtgac atagtcggcc
     1621 gcgcggttct ggcgcagggc ggcgatgact cccagcgaca ccccgaccag cgccgccagc
     1681 aggatcgaca gcccgcccag cgtcaggctg accctgtagt tgtcctgcag gatctcgagc
     1741 acggtcttgt cgcggtattt cagcgacggg ccgaaatcgc cgacggccac gcccttgaga
     1801 tagttgacgt attgttcggt cagcggccga tccatgccga acttggccat caccgccttc
     1861 tcgacctcgg gcgaaagctg ccgctcgttg accagcggac tgcccggcgc cgcccgcatc
     1921 atgaagaagg cgacggtgat gaccagcaga agcgtgggga tggccacgag caaccggcgg
     1981 aacaggaaac gcagcatcgt tggtcactcg cgcggccggg ccgcaaaggc gttgcgaaac
     2041 ttccatccgg ccccgatgtt gtcaacgaaa ccccagccgg gatgaaccag acatgaccga
     2101 attccgccgc gtgaccgatg acttctccgt cagcccgcag atcacgcccg aggccatggc
     2161 cgcagccgcg gcccagggct tcaccctggt gatcaacaat cgtcccgacg gcgaagtccc
     2221 cgaccagccg accagcgcgc agatggaggc cgccgcccgg gccgccggca tggactatgt
     2281 ccacaacccg gtgaccggca gtccgaccca ggaccaggtc gaggcgcagc ggctggcgat
     2341 cgaggacgcc gacggcccgg tgctggcctt ctgtcgctcg ggcacccgat cgatcgtcac
     2401 ctggtcgctg ggccaggcgc tgtcgggcgc gcggtcgcgc agcgaactgg cggcgctcgg
     2461 gcgccaggcg gggtacgatc tgtcgggggt gctggggtag gtttcgcgcc gggcggcgag
     2521 gatcgccgtc agaccccgcc cagggtcacc cgggcagcca tcgccgaggg ctccggctcg
     2581 cagctggcga ggaccagttg cgccgtgacg agacagagaa aggcgaacgc cgccctctgg
     2641 agccgttttg agacaaagat cgccgcatcc gccgccattt tggcgtcctc ccgcactgcc
     2701 atgcgggacg cagcgcaaga gcggcgccgc gcggccccat cacggggccg tcacgggcac
     2761 gtcgcttgcc tgtgatcgaa actccattag ttaacggcgt cggtgccgcg ggggcggcga
     2821 aagggattga atggccggga cgctgtggac gtccgatttc cacttcgaga gtgggcgggt
     2881 gaaggtgcgg accacgggcg cggacctggc gctggaccgc gccctgctgg gcgacgtcgg
     2941 cctgtggctg gcctattacc tgcccgtgcg gctgaagggg ctggtgaccg ccgtcgcgcg
     3001 gcccgggccg cggatctggt tcgcccccga cgcgccccgg ccgtggtacc tgctgggcct
     3061 ggccgcctgc tgggccgggg tgcgcgtcgc ctcgtcgccg gacaaggccg acgcggccgt
     3121 ctatttcgag gacgtgaccg tcgccgcccc gccagcctgt gacctgcccc ggcggttcaa
     3181 cttcgcctgc ggcgacgttt ccaagagccg cgtcgcggcg gtgttcgagc aggtgttcgg
     3241 ctatccgctg gccgtcgatc cgacgacctg gcgggggctt gccgtggaaa agggcgaggg
     3301 caacggcgcg catgacgggc ggatcgtcca gtgtccctgc cccgcccagc ccgggcgcgt
     3361 ctaccagcgc gtggtcgaca ccgtcgagga cgaccatgtc gtcgacctgc gcacgccctg
     3421 cgtcggcggc gcgccggtgc tgaccttcat caagcggcgg ccggcgcgcg accggttcgc
     3481 caactacaac gcctcggtct cgctgcatcg ccccgacgag ctgttcacgc cgcaggagct
     3541 ggccgtcatc gaacggttct gcgcggccat gaacctcgac tggggcggac tggacatcct
     3601 gcgcgaccgg gccgacggcc ggctgtatat cgtcgacgtc aacaagacgg acatgcctcc
     3661 actccggctg ccctggcggc agaagatgga agcgatagcg agactggcga aggcgtttct
     3721 ggttatggtt gacggcgcaa cggcaagggg gagcgcatga tcccgttcgt ccgcgacatc
     3781 aggttcgact acggcgcctg cgaccggctc acgccgctga tccggcgcgt ggtggccaac
     3841 aatcccggac cgttcacctt caccgggacc ggcacctaca tcatcggcaa cggcgaggtc
     3901 gcggtgatcg atcccggccc cgacatggcc gagcacctcg acgcgatcct ggcggccacg
     3961 gccggcgagc ggatcagcca catcttcgtc acccacaacc acatggacca ttcgccgctg
     4021 gcccggccac tggcggcggt caccggcgcg accatctacg cctgcgccat gcagggcgtg
     4081 gcgaccgagt ccgaggtgag gctcgaggcc ggcgacgacc gggtgttcac gcccgatgtc
     4141 ggcctgtgcg acggcggggt cttcaagggc ggcgactgga cgctggaggc gatccccacg
     4201 ccgggccaca cctccaacca catctgctac gcgctgatcg aggagaacgc gctgttctcg
     4261 ggggaccaca tcatgggctg gtcgaccacc gtgatcagcc cccccgacgg cgacatgggc
     4321 gactatttcg ccagcctcga acgggtcaag gcgcgcgact tcgcgaccat ctggccgacg
     4381 catggtccgc cgatcaccgc gaccgggccg ttcgtccagg cctacatcga ccaccgccgc
     4441 gcgcgcgagg cgcagatcct cgcggccctg ggctccggtc acacgcggat caagccgatg
     4501 gtcgccgacc tgtaccgcga ggtcgacgcg cggctgcacc cggccgccgc ccattcggtg
     4561 atggcccaca tgatccagct ggtgaaggag ggccgcgtcg tctgcgacgg cccgcccggc
     4621 ctagacagcg actaccgcct ggcggggtga caccccgcgg ctcccctccc ccgggaggcg
     4681 gccagatgga gacgggttat ccccgaatgg ccctcctcct cctccatcgg gggaggaaga
     4741 gagtccatcg cgcccggccg ccgggatggg gcgaccctat cccgcgatcg ccgtaaccag
     4801 ggtcccgatg cgtcggcagt tggcgcccag gtcgctgtct ccggtgcggc gcacggcgcg
     4861 caggtcgatg cgcgcgccgg cccccgcggg gcggacgcgc accaccacgt cgtccttgaa
     4921 gccgtagagc aggctgaccg ccgtcgcctc gatgcgaccg gaagcggcgt cggcggtgat
     4981 gatcgacatg ccggcccgct cgacggccga gcgggcatgg gcgaaggcct gggcggccga
     5041 gatggccacc gtcaccggtc gcgcggccgg acaggtctcc gcgttgacct cggccacccg
     5101 tcggccggcg taggcctgcg agccgagggg caggaccggc gaggggtcga cgggattggc
     5161 gtcgggaccc ctggccttca gcgcggcggg cggtgcggtc tttttctttt tccagcgact
     5221 cgcgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagaagcc
     5341 gccgaacacc gccaccagca cgccgaccag gcccgtcagc accccgacca gggcgatcgc
     5401 cggcgcccag ccgagcgact cggtcggccc ctggaccagc atcgcctcga agccctgctg
     5461 cggggtgatc aggttgaact tggcggccag ggcgccgaac agccacatcg ccgccggcgc
     5521 cagggcgacc agcagggcga aatcgagata gcggccggcc cagccgccgc cggcggcctt
     5581 c
//
LOCUS       DAORNX010000167         5507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10958_length_5507_cov_7.086207, whole genome shotgun sequence.
ACCESSION   DAORNX010000167 DAORNX010000000
VERSION     DAORNX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_5507_cov_7.086207"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLO65_08575"
     CDS             complement(<1..631)
                     /locus_tag="PLO65_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRD28341.1"
                     /translation="MRWILRGLLTIVGLMLGVAATVAAADRLTAPPRPALTPLIEVAR
                     NYDVVIRRDRFGVPHILGKTDADAAFGLAFAHAEDDFETIQTVALATRGTLAARDGPK
                     AAPGDYIVSLLGVWNTVNARYDRDLPPDVKAVLQAYADGINYYAARNPGKVAPGLLPL
                     TGKDIAAGFVFKTPFFYGLDGELQRLTRATSDGLPKGSNGVAVAPSRSSD"
     gene            856..2703
                     /locus_tag="PLO65_08580"
     CDS             856..2703
                     /locus_tag="PLO65_08580"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRD28342.1"
                     /translation="MGGETTVPGLDQAPTRHARLLAWVRDVAALTKPDRVHWCDGSDE
                     EWVALTDQLVEAGTLKRLDPVKRPNSFFAASDPRDVARVESRTFICSANEADAGPTNN
                     WLDPEEMRADLNNLFDGCMRGRTMYVVPFSMGPIGSPISALGVEITDSAYVAISMRVM
                     TRMGKQALDALGDDGFFVPALHSVGAPLEPGQTDVPWPCNEIKYIVHFPESREIWSYG
                     SGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILKLTNPRGEVRFVAAAFPSACGK
                     TNLAMLQPTIDGWKAETIGDDICWMRFGEDGRLYAINPEAGFFGVAPGTGMGTNRNAI
                     EALHQNTVFTNVALTDDGDVWWEGLTDDKPARLTDWRGRPWTPESPEPAAHPNARFTV
                     PAGQCPVIASEWDDPRGVPISAILFGGRRASAVPLVTEAFDWAHGVFMGSTVASEGTA
                     AAENKVGELRRDPFAMLPFCGYNMGDYFGHWLKVGGNADAAKLPRIFMVNWFRKDENG
                     RFVWPGYGENSRVLKWIFDRLEGEAEAVDSPIGRVPAPGSLDTVGLGLTEAQIRLLKT
                     VDVDIWREEASLIPAFYEKFGDRMPAELWSQLKALKARLEAAARVVEPA"
     gene            complement(2887..4824)
                     /gene="mdoH"
                     /locus_tag="PLO65_08585"
     CDS             complement(2887..4824)
                     /gene="mdoH"
                     /locus_tag="PLO65_08585"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRD28343.1"
                     /translation="MDGTSNLAIRYDAPAYAPSYASAPEREHLPTPAPLAMPRQPLID
                     GGVMSSRIPGTSPTDIARRRVILFTATLLLTALASFEPVRMYAKDGFLPLEIIGLALF
                     EVLITAISCWFCSAAIGLTLLLIRGDRDELSFAAVAPRPTVRTALLMPLYNEDAQASF
                     GRLAQIERSLSRLNASQAFDIFVLSDSTDERAATSEWAAFQIFRLQSSCRVYYRRRRE
                     NTERKVGNLSDWVRRFGAAYSHMIVLDADSTMAGETILHLVDAMERHPGIGLIQTTPT
                     IVEGKTLFARASQFGVRLYGRVASAGLAWWSGSEGSYWGHNAIVRVRAFADCAGLPVL
                     PGRKPFGGPLMSHDVVEAGLLRRGGWGVHVTPALSGSHEETPPSLLEFMKRERRWCQG
                     NLQHIALLRAPGLHWMSRFQLIVGLLAYLASPIWLLSLAAGLLIQVQNKADWGSWWYF
                     LHPEVTPALLAGLLTTGMLLGPKLMGVALVLSRPAERRAFGGTRTLLKGVAAEMGLSA
                     LTAPIYMIGNTRAVFEILLGKDAGWVSQNRDADGLSLVDAKVAFRNEFFAGLFFLVSL
                     AMRPDLLLWIWPIFVPMLFAGRIAAWTSSRAAGDRARASGLLLTPEEIAAAGRVAAPH
                     SGAEVVPMPVRAVDPMAIAAE"
     gene            complement(5070..>5507)
                     /locus_tag="PLO65_08590"
     CDS             complement(5070..>5507)
                     /locus_tag="PLO65_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28344.1"
                     /translation="GIAGIGFGLATLALGGPRAPAALTALAFVLAAVGLVVSSLYHWP
                     DPRHLAINLGLGIQLAPLFLLWGLARADGVSRLRWFLAAVFVLMAVLTVITKHLVFPG
                     TVNPQNVGWWERAFAVVLVGWVGVAAFLLERRLIALAAEGDGS"
BASE COUNT          908 a         1863 c         1958 g          778 t
ORIGIN      
        1 cgtccgacga acgcgacggg gccacggcca ccccgttgga gcccttgggc aggccatcgg
       61 atgtggccct ggtgagccgc tgcagctcgc cgtccaggcc gtagaagaac ggagtcttga
      121 acacgaagcc ggcggcgatg tccttgccgg taagcggcag caggcccggc gcgaccttcc
      181 cgggattgcg ggcggcgtag tagttgatcc cgtcggcgta ggcttgcagc acggccttca
      241 cgtccggcgg caggtcgcgg tcatagcggg cgttgaccgt gttccacacg cccagcaggc
      301 tgacgatgta gtcgccgggc gcggccttcg gcccgtcgcg ggcggcgagg gtcccgcgcg
      361 tggccagggc gaccgtctgg atggtctcga agtcgtcctc ggcatgggcg aaggccaggc
      421 cgaaggcggc gtcggcgtcg gtcttgccca ggatgtgcgg cacgccgaac cggtcgcgcc
      481 ggatgaccac gtcgtagttg cgggcgacct cgatcagcgg cgtcagggcg gggcggggcg
      541 gcgccgtcaa ccggtcggcg gcggccacgg tggccgccac gcccagcatc aggccgacga
      601 tcgtcagcag accccgcaag atccagcgca tgcccgtttc ccccgtgccg ccgcgatcct
      661 agcgcggcgg cgcgcgaaat ccatgccgcg gctggttttg ccgccgccat cggcgcggct
      721 gcaaatcacc cgcatgggcg tttcggcttg cgacaacgcc tcccggcggg ctaggcaggt
      781 ggcagaaaag tcgatgaccc cagccgcgga agacggaccg ggcgacgacg tctgtagttc
      841 aggaggacgc cggtgatggg cggcgagacc acggttccag gccttgatca ggcccccacc
      901 aggcatgcgc gcctgctggc ttgggtgcgc gatgtcgccg cgctgaccaa gcccgatcgc
      961 gtccactggt gcgacggctc cgacgaggag tgggtcgccc tgaccgatca actggtcgag
     1021 gccggcaccc tcaagcgcct tgacccggtc aagcgcccca acagcttctt cgccgcttcc
     1081 gatccgcgcg acgtggcccg cgtcgagagc cgcaccttca tctgctcggc caacgaggcc
     1141 gacgccgggc cgaccaacaa ctggctcgac ccggaggaaa tgcgggcgga cctgaacaac
     1201 ctgttcgacg gctgcatgcg cggccgcacc atgtatgtcg tgccgttcag catgggcccg
     1261 atcggctccc cgatcagcgc cctgggtgtc gagatcaccg acagcgccta tgtggcgatc
     1321 tcgatgcggg tcatgacccg catgggcaag caggcgctgg acgcgctggg cgacgacggc
     1381 ttcttcgtgc cggccctgca ctcggtcggc gcgccgctcg agccgggcca gaccgacgtc
     1441 ccctggccgt gcaacgagat caagtacatc gtccacttcc cggaaagccg cgagatctgg
     1501 tcgtacggat ccggctacgg cggcaacgcg ctgctgggca agaagtgcta cgccctccgc
     1561 atcgccagcg taatggcccg cgacgagggg tggctggccg agcacatgct gatcctcaag
     1621 ctgaccaacc ccaggggcga ggtccgcttc gtggccgccg ccttccccag cgcgtgcggc
     1681 aagaccaatc tggccatgct ccagccgacg atcgacggct ggaaggccga gaccatcggt
     1741 gacgacatct gctggatgcg attcggcgag gacggccggc tctacgccat caatccggag
     1801 gccggcttct tcggcgtggc gccggggacc gggatgggca ccaaccgcaa cgccatcgag
     1861 gcgctgcacc agaacaccgt gttcaccaac gttgccctga ccgacgacgg cgacgtctgg
     1921 tgggaaggcc tcaccgatga caagcccgcg cgcctgaccg actggcgcgg ccggccctgg
     1981 accccggaaa gcccggagcc ggcggcccat cccaacgccc gcttcaccgt gcccgccggc
     2041 cagtgcccgg tcatcgccag cgagtgggac gatccccgcg gcgtgccgat cagcgccatc
     2101 ctgttcggtg gccggcgcgc gagcgccgtg ccgctggtca ccgaggcctt cgactgggcc
     2161 cacggcgtgt tcatgggctc gaccgtcgcc tccgaaggca cggcggcggc cgagaacaag
     2221 gtcggtgagt tgcgccgcga cccgttcgcc atgctgccgt tctgcggcta caacatgggc
     2281 gactatttcg gccactggct gaaggtcggc ggcaacgccg acgccgcgaa gctgccgcgc
     2341 atcttcatgg tcaactggtt ccgcaaggac gagaacggcc gcttcgtctg gcctggctac
     2401 ggcgagaaca gccgggttct gaagtggatc ttcgaccggc ttgaaggcga ggccgaggcg
     2461 gtcgattcgc cgatcggccg cgtgccggcg ccgggctcgc ttgataccgt gggcctgggc
     2521 ctgaccgagg cccagatcag gctgctcaag accgtcgatg tcgacatctg gcgcgaggaa
     2581 gcctcgctca tcccggcctt ctacgagaag ttcggcgacc ggatgccggc ggaactgtgg
     2641 agccagctca aggcgctcaa ggcgcgcctg gaggccgccg cacgggttgt cgaaccggcc
     2701 tgagcggctt cccagaagcg ctggttggtc tgtcgcgata acgcagacct tccggccctg
     2761 cttttcggtc ttcggcggtc gaaggcgagg cccgaaagtc aggccggccg actgatctgt
     2821 cggaagcttg tcggacccgc cgctgcgaga tgatcggcgc ggctttccag tctgccggta
     2881 gggcgattac tccgccgcga tcgccatcgg gtcgacggcg cggaccggca tgggcaccac
     2941 ttccgcgccg ctgtggggcg cggcaacgcg gccggcggcg gcgatctcct cgggagtcag
     3001 cagcaggccg ctggctcggg ccctgtcgcc ggcggcgcgg ctggaggtcc aggcggcgat
     3061 ccgcccggcg aacagcatcg gcacgaagat cggccagatc cacagcagca ggtccggccg
     3121 catggccagg gacacgagga agaacaggcc ggcgaagaac tcgttgcgga acgcgacctt
     3181 cgcgtcgacc aggctgagcc cgtcggcgtc gcggttctgg ctgacccagc cggcgtcctt
     3241 gcccagcagg atctcgaaca ccgcccgggt gttgccgatc atgtagatcg gcgcggtcag
     3301 ggccgacaga cccatttccg cggcgacgcc cttcagcagg gttcgcgttc ccccgaaggc
     3361 gcggcgttcg gccgggcggc tcaggacgag ggccacgccc atcagcttgg gacccagcag
     3421 catgcccgtg gtcaacagcc ccgccagcag ggccggggtg acctcgggat ggaggaaata
     3481 ccaccacgag ccccagtcgg ccttgttctg gacctggatg agcaggccgg cggccaggga
     3541 cagcagccag atcggggagg ccagataggc caacaggccg acgatcagct ggaagcggct
     3601 catccagtgc aggcccggcg cgcgcagcag ggcgatgtgc tgcaggttgc cctgacacca
     3661 gcgacgctcg cgcttcatga actccagcag gctgggcggg gtttcctcgt ggctgcccga
     3721 cagggccgga gtgacatgca cgccccagcc gccgcgacga agcaggccgg cctcgaccac
     3781 gtcgtggctc atcagcggcc cgccgaacgg cttgcgtccc ggcagcaccg gcaggccggc
     3841 gcagtcggcg aaggcgcgga cgcggacaat ggcgttgtgg ccccagtagc tgccttccga
     3901 cccggaccac caggccaggc ccgccgaggc gacgcggccg tagaggcgca cgccgaactg
     3961 tgaggcgcgg gcgaacaggg tcttgccctc gacgatcgtc ggcgtggtct ggatcaggcc
     4021 gatgcccgga tggcgttcca tggcgtcgac caggtgcagg atggtctcac ccgccatggt
     4081 gctgtctgcg tcgagcacga tcatgtggct gtaggcggcg ccgaagcggc gaacccagtc
     4141 cgacaggttg ccgaccttgc gctcggtgtt ctcgcggcgg cggcggtagt agacccggca
     4201 actggactgc aggcggaaga tctggaacgc cgcccactca ctggtcgcgg cccgctcgtc
     4261 ggtgctgtcg ctgagcacga agatgtcgaa cgcctggctg gcgttgagcc gcgacagcga
     4321 acgttcgatc tgcgccagcc ggccgaacga ggcctgggcg tcctcgttgt acaggggcat
     4381 cagcagggcg gtgcggacgg tcgggcgggg cgccacggcg gcgaacgaca gctcgtcgcg
     4441 gtcgccgcgg atcagcaaca gggtcaggcc gatcgccgcg ctgcagaacc agcaggagat
     4501 ggccgtgatc aggacctcga acagcgccag accgatgatc tcgagcggaa ggaagccgtc
     4561 cttggcgtac atccgcacgg gctcgaacga ggccagggcg gtcagcagca gggtggcggt
     4621 gaacaggatg acgcggcgac gggcgatatc ggtcggcgag gtccccggga tgcgggacga
     4681 catcacgccg ccgtcgatca gcggctgtcg cggcatggcc agcggcgccg gcgtcggcag
     4741 gtgctcgcgt tccggcgccg acgcatacga cggggcgtaa gcgggagcgt cgtaccggat
     4801 cgccagattg ctcgtgccgt ccatgaaggg ccgccgtgat cgagagggaa tccggagcca
     4861 gatggcctcg gcgttgcgat cacgcttatg ttatgctgcg gcgcacccta gcaaggggtt
     4921 ttgtcggaaa cctgtgcaga aaacgcgatt tttccgtgcg gcgccgcgat ttttgtcgtg
     4981 atcagttcgt gatcgcgcgc cgttcacgca aatgagaggc gggcgcgacg atcatcggac
     5041 cgaccgatgg acgactcgcc ggatgagggt cagcttccgt cgccttccgc cgccagcgcg
     5101 atgagtcggc gctcgagcag gaaggccgcc acgccgaccc agccgaccag caccacggcg
     5161 aaggcccgct cccaccagcc gacgttctgc ggattgaccg tccccggaaa caccaggtgc
     5221 ttggtgatca ccgtcagcac cgccatcagc acgaacacgg cggcgaggaa ccagcgcaga
     5281 cgcgaaacac cgtcggcccg ggcaaggccc cagagcagaa acagcggagc cagctgaatg
     5341 ccgagtccga gattgatcgc caggtggcgc gggtccggcc agtgatagag gctggagacc
     5401 accaggccca cggcggccag cacgaaggcc agggcggtca gggccgccgg cgcgcggggc
     5461 ccgcccaggg ccagggtcgc caggccgaag ccgatgccgg cgatgcc
//
LOCUS       DAORNX010000168         5505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_10960_length_5505_cov_8.132110, whole genome shotgun sequence.
ACCESSION   DAORNX010000168 DAORNX010000000
VERSION     DAORNX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_5505_cov_8.132110"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLO65_08595"
     CDS             <1..758
                     /locus_tag="PLO65_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRD28345.1"
                     /translation="ADAAAAPAPAPEAPAAAPAPKKESHITPVTMFMDAKAVVKVVML
                     GLLLASVFSWTLLVTKLLEFGSLNRKSDRFVEAFRGAKSIGDMGRIALSDEFEGNPLA
                     DMTAAAASEVELSRQAGLAIGGAHRDTTIMRAQTAVSAVQSSLSKRLSSGMQFLASVG
                     SSGPFIGLFGTVYGIMNSFIGIAESNTTNLAVVAPGIAEALLATGIGLFAAIPAVIFY
                     NYFQTRISSYGVRSDGFNAELMNNISRQLDKGA"
     gene            765..1217
                     /locus_tag="PLO65_08600"
     CDS             765..1217
                     /locus_tag="PLO65_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRD28346.1"
                     /translation="MAAKLSGSGGGSRYNVEQNSEINVTPFVDVMLVLLIIFMVAAPL
                     ASVSIEVKLPTAVAKPRPNPPTPIYISIQPGGAVFIGDNPTDLASMGDDLRKQIRVRN
                     PSKERIFIRADKAVRYGDFMQVMNTLQDNGFYSVALIGQDDSLPGNGG"
     gene            1221..1940
                     /locus_tag="PLO65_08605"
     CDS             1221..1940
                     /locus_tag="PLO65_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRD28347.1"
                     /translation="MAEHENDAPHRVHNPFDAPAPKNGGGFMVGLALALALNAGLIFW
                     VAKSRFEIKEKLFEDEKLDTQLIKPPPPPPPPPPPPPPSEKPPPPAKLQPRPPAIVPP
                     DVAPPPPLQVPPVEKKDRVETTAPPVIAPPSPPTPPRPSVISNPDWARRPSGDDIARF
                     YPDRAQRLEKEGRATLLCRVKANGTLEGCEVVSEDPADLGFGDAALKLSRLFKMRPQT
                     RDGAPVDGASVRIPLVFKLPQ"
     gene            complement(2041..2931)
                     /locus_tag="PLO65_08610"
     CDS             complement(2041..2931)
                     /locus_tag="PLO65_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28348.1"
                     /translation="MSEIRTRTLQANGLSFVIDEAGEGENVALFLHGFPESKHSWRHQ
                     LPLLASLGWRAVAVDQRGYGDSSRPRGVAAYDIEHLMADAGAVFDALGAKRRLLIAHD
                     WGAIVAWCFAMDRVRDLEGLVIMNVPHPAVFQQVVRASPAQRRRSWYVAFFQIPWLPE
                     KVLTARGAEAIGRAFSDMAVDKSRFSAADLDHYRKNALRPGAATAMVNWYRAGARNGL
                     KKWAPDRAPVIDVPTLMIWGEEDAALGIELTEGYEPYVKDFTLQRLPGVSHWVQQEAP
                     EAVNARLEAWLRAKGLQGTA"
     gene            3023..3099
                     /locus_tag="PLO65_08615"
     tRNA            3023..3099
                     /locus_tag="PLO65_08615"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3057..3059,aa:His,seq:gtg)
     gene            3186..4130
                     /locus_tag="PLO65_08620"
     CDS             3186..4130
                     /locus_tag="PLO65_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRD28349.1"
                     /translation="MIDNRITRLLGVQYPIVQAPMGWIARARLASAVSNAGGLGIIET
                     SSGRLDEVREEVKKMRGLTDKPFGVNIAQAFVRDPDIVSFVVDQGVKFVTTSAGDPNK
                     YCGPLKEAGLTVFHVVPSLSAALKALAAGVDGLVVEGGEGGGFKNPREVALMVLLPLI
                     ASRTDKPIIAAGGMMDGRTMAAAFALGAEGVQMGTRMVSAAESPVHQNWKDAIVNAAE
                     TDTVFLNRFGPGPALRALRTGKTTEMEINPPAAGAMPEMARALELYFGGDMEASIALS
                     GQVAGRIDSVRPVADIIADTVREFHEVCAGLGAKYGKA"
     gene            4270..>5505
                     /locus_tag="PLO65_08625"
     CDS             4270..>5505
                     /locus_tag="PLO65_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HRD28350.1"
                     /translation="MGRGWNRALGALTIAGALALAACGPGGGSAAKPNTTYQETLIQQ
                     LIDAKPGDVITIPAGVYAIDRGLSLDVDGVTIRGAGMDRTILSFKGQAAGAEGLLVNA
                     SNFTIEDLAIEDAKGDGLKVNEGENIIIRRVRAEWTGGPKTSNGAYGLYPVQVRNLLI
                     EDSVAIGASDAGIYVGQSNNVVVRRNRAEFNVAGIEIENTQNADVYDNLAINNTGGIL
                     VFNMPNLKVSGFATRVFRNRVEGNNTGNFGRKGSAVASVPAGTGVIINANDQVEIFDN
                     DIGGNNTANVIISSYFSTGYMSKEGVAPNFDPWPESIFVHDNRFTPGGAKPDGLDLQA
                     LRVAMFGVGGRLPDVLWDGYVDVKKFKGGKVDPALAICVDNGAAQTLNADGPHRNKSP
                     ALAGEALRCRLAPLPAISVA"
BASE COUNT          915 a         1888 c         1825 g          877 t
ORIGIN      
        1 ccgctgacgc cgccgctgcg cccgcccccg cgccggaggc gccggccgcg gctcccgcgc
       61 ccaagaagga atcgcacatc acgcccgtca ccatgttcat ggacgccaag gcggtcgtga
      121 aggtggtcat gctgggcctg ctgctggcct cggtcttctc ctggaccctg ctggtcacca
      181 agctgctcga gttcggttcg ctgaaccgca agtcggaccg cttcgtggaa gccttccgcg
      241 gcgccaagtc catcggcgac atgggccgta tcgccctgtc cgacgagttc gaaggcaacc
      301 cgctggccga catgaccgcc gcggcggctt ccgaagtgga gctttcgcgt caggccggcc
      361 tggccatcgg cggcgcccac cgcgacacca cgatcatgcg cgcgcagacc gccgtgtccg
      421 ccgttcagtc gtcgctgtcc aagcgcctgt cgagcgggat gcagttcctc gcctcggtcg
      481 gttcgtccgg tccgttcatc ggtctgttcg gcaccgtcta cgggatcatg aactcgttca
      541 tcggcatcgc cgaatcgaac acgaccaacc tggccgtcgt ggcccccggc atcgccgagg
      601 ccctgctcgc caccggtatc ggtctgttcg ccgctatccc cgcggttatc ttctacaact
      661 acttccagac gcggatttcg tcgtatgggg tgcggtcgga tggtttcaac gccgagttga
      721 tgaacaacat ctcgcggcag ctcgacaaag gggcttaagc acagatggcc gccaaactct
      781 cgggctccgg cggcggcagc aggtacaatg tagagcagaa cagcgaaatc aatgtgacgc
      841 ccttcgtgga cgtcatgctg gtcctcctga tcatcttcat ggtggctgcg ccgctggcat
      901 cggtctcgat cgaggtaaag ctgcccacag cggtggccaa gcctcgaccg aacccgccaa
      961 cgccgattta catttcgatc cagccgggcg gggccgtctt cattggcgac aacccgaccg
     1021 acctggcctc gatgggcgat gatcttcgga agcagattcg cgttcggaac ccctcgaagg
     1081 agcggatctt catccgcgcc gacaaggctg tccgctacgg cgacttcatg caggtgatga
     1141 acacgttgca ggacaatggc ttctacagcg tggcgctcat cggccaggat gacagccttc
     1201 cgggcaatgg tgggtgacgc atggcagagc atgaaaacga cgctccgcac cgcgtgcaca
     1261 accccttcga cgctccggcg ccgaagaacg gtggcgggtt catggtcgga ctcgcgctgg
     1321 ccctggcgct caatgcggga ctgatcttct gggtcgccaa gtctcgtttc gagatcaagg
     1381 agaagctgtt cgaggacgag aagctggaca cccagctgat caagccgcct ccgccgccgc
     1441 ctccgccgcc gccgccgcct ccgccctcgg agaagccgcc gcccccggcc aagctgcagc
     1501 cgcgtcctcc ggccatcgtg ccgcctgacg tcgcgccgcc gccgccgctg caggtgccgc
     1561 cggtggagaa gaaggaccgc gtcgagacca cggctccgcc cgtgatcgcg ccgccctcgc
     1621 ctccgactcc gccgcggccg tcggtcatca gcaacccgga ctgggcccgt cggcccagcg
     1681 gggacgacat cgcccgcttc tatccggacc gcgcccagcg gctggagaag gaagggcgag
     1741 cgacgctcct ctgccgggtc aaggcgaacg gcacgctgga aggctgcgag gtggtttcgg
     1801 aagatcccgc cgacctcggc ttcggcgacg cggccctcaa gctgtcgcgt ctgttcaaga
     1861 tgaggccgca gacgcgcgat ggcgcgccgg tggatggggc ttcggtccgt atcccgctgg
     1921 tgttcaagct gccgcaatag gttgtgtatc caacccggat gcgaacgccc cggagggaaa
     1981 cctccggggc gttttgctgt gtgcggccac tcaaaatcgg ggtcgtcggg cggggacagc
     2041 tcaagcggtt ccctggagcc ccttcgcccg cagccatgcc tccagccggg cgttgaccgc
     2101 ctccggcgct tcctgctgga cccaatgcga cacgccgggc aggcgctgca gggtgaagtc
     2161 cttcacatag ggctcgtagc cctcggtcag ctcgatcccc agggcggcgt cctcctcgcc
     2221 ccagatcatc agggtcggga cgtcgatcac tggcgccctg tccggcgccc acttcttcag
     2281 gccgttccgg gccccggcgc gataccagtt gaccatggcc gtcgcggcgc cgggcctgag
     2341 cgcgttcttg cggtagtggt cgaggtcggc cgcgctgaag cggctcttgt ccaccgccat
     2401 gtcgctgaag gcccggccga tggcctccgc gccccgggcg gtcagcacct tttcgggcaa
     2461 ccaggggatc tggaagaagg cgacgtacca ggacctgcgc cgctgggcag gactggcgcg
     2521 gacaacctgc tggaagaccg ccgggtgcgg cacgttcatg atcaccaggc cctcgaggtc
     2581 gcgcacccgg tccatggcga agcaccaggc gacgatcgcg ccccagtcgt gggcgatcaa
     2641 cagccgtcgc ttcgccccca gggcgtcgaa caccgccccc gcgtcggcca tcaggtgctc
     2701 gatgtcgtag gcagccacgc cccggggtcg acttgagtcg ccgtagccgc gctggtcgac
     2761 ggccaccgcc cgccagccga ggctcgccag cagcggcagt tgatgacgcc agctgtgctt
     2821 gctctcggga aagccgtgca ggaacagggc gacgttttcg ccttcgccgg cctcgtcgat
     2881 gacgaacgac aggccgttgg cctgcagggt gcgggttctg atctcgctca tcgggcaagg
     2941 gtggcggccg tgcggcttgt gcgcaagcgc cgcgcttgat cgaacggccc ggagcgacta
     3001 aacccgccct tcgcgaaagc gcgccgcctt agctcagttg gttagagcgc cggtttgtgg
     3061 agccggaggt cctcagttcg agcctgagag gcggtaccat cgcaaggcct gaccggggcg
     3121 ctccggccac acttccaaac aactgtttga gtctgtagcg tcgacccaac cctggggaga
     3181 cgaccatgat cgacaaccgc atcacccgac tgctcggcgt ccagtatccg atcgtgcagg
     3241 ccccgatggg ctggatcgcg cgggcccggc tagcgtcggc ggtgtccaac gccggcgggc
     3301 tcggcatcat cgagacctcc tccggccgcc tcgacgaggt ccgcgaggaa gtgaagaaga
     3361 tgcgcggcct gaccgacaag ccgttcgggg tgaacatcgc ccaggccttc gtgcgcgacc
     3421 ccgacatcgt cagcttcgtc gtcgaccagg gcgtgaagtt cgtcaccacc tccgccggcg
     3481 atccgaacaa gtactgcggt ccgctcaagg aagccggcct gaccgtgttc cacgtggtgc
     3541 cctcgctgtc ggcggccctc aaggcgctgg cggccggcgt tgatggcctg gtcgtcgaag
     3601 gcggcgaggg cggtggcttc aagaacccgc gcgaggtggc gctgatggtg cttctgccat
     3661 tgatcgccag ccggaccgat aagccgatca tcgccgccgg cggcatgatg gacggccgaa
     3721 ccatggccgc cgccttcgcc ctcggcgccg agggcgtgca gatgggtacg cgcatggtct
     3781 cggccgccga gtcgccggtg caccagaact ggaaggacgc gatcgtcaac gcggccgaaa
     3841 cggacacggt gtttctcaac cgcttcggtc cggggccggc gctgcgggcg ctgcgcacgg
     3901 gcaagaccac cgagatggag atcaatcccc cggccgccgg cgccatgccc gagatggcca
     3961 gggcgctgga gctctatttc ggcggcgaca tggaagcctc catcgccctg tccggccagg
     4021 tggcgggccg catcgacagc gtccgcccgg tggccgacat catcgccgac acggttcgcg
     4081 aattccacga ggtctgcgct gggctgggcg cgaagtacgg caaggcgtag ccgccctttc
     4141 cttcgtcatg gcccgaacaa gtcgggccat gacgtctgtg gggtgattgc ccctctctgt
     4201 cccccatgcg aggttcccgt cggaaccggg gccgccagag tcccggccgg acggaaaggg
     4261 agacgatcca tggggcgggg ctggaatcgc gcgctgggcg cattgaccat cgcgggggcg
     4321 ctggccctgg cggcctgcgg gccgggtggc ggttcggcgg ccaagcccaa caccacctac
     4381 caggaaaccc tgatccagca gttgatcgac gccaagccag gcgacgtgat caccattccc
     4441 gccggggtct acgccatcga ccgcggcctg agtctcgacg ttgacggcgt cacgatccgc
     4501 ggcgcgggca tggacaggac cattctgagc ttcaagggtc aggcggccgg ggccgagggg
     4561 ctgctggtca acgccagcaa cttcaccatc gaggacctgg ccatcgagga cgccaagggc
     4621 gacggcctga aggtcaacga aggcgagaac atcatcatcc ggcgggtgcg cgccgaatgg
     4681 acgggcggcc cgaagaccag caacggggcc tacggcctct atcccgtaca ggtgcgcaac
     4741 ctgctgatcg aggacagcgt ggccatcggc gcgtcggacg cgggcatcta cgtcggccag
     4801 tcgaacaatg tcgtcgtgcg ccgcaaccgg gccgagttca atgtcgccgg catcgagatc
     4861 gagaacaccc agaacgccga cgtctatgac aacctggcga tcaacaacac cggcggcatc
     4921 ctggtgttca acatgcccaa cctgaaggtt tcgggctttg ccacccgcgt gttccgcaac
     4981 cgggtcgagg gcaacaacac cggcaacttc ggccgcaagg gctcggccgt ggcctcggtg
     5041 ccggcgggca ccggggtgat catcaacgcc aacgaccagg tagagatctt cgacaacgac
     5101 atcggcggca acaacaccgc caatgtgatc atctccagct acttctcgac cggctacatg
     5161 agcaaggaag gggtggcccc gaacttcgac ccctggccgg agtcgatctt cgtccacgac
     5221 aaccgcttca cgccgggcgg ggccaagccc gacggactcg acctgcaggc cttgcgggtg
     5281 gcgatgttcg gcgtcggcgg ccggctgccc gacgtgctgt gggacggcta tgtcgatgtg
     5341 aagaagttca agggcggcaa ggtcgatccg gcgctggcga tctgcgtcga caacggcgcg
     5401 gcccagaccc tcaacgccga cgggccgcac aggaacaagt cgccggccct ggcgggcgag
     5461 gcgctgcgct gccgcctcgc gccgctcccg gcgatcagcg tggcg
//
LOCUS       DAORNX010000169         5488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11004_length_5488_cov_6.812258, whole genome shotgun sequence.
ACCESSION   DAORNX010000169 DAORNX010000000
VERSION     DAORNX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_5488_cov_6.812258"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLO65_08630"
     CDS             complement(<1..271)
                     /locus_tag="PLO65_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRD28351.1"
                     /translation="MTDPALAAQKATLRMEMRNRRKGLLVEHPEADWMAADRAGEMLA
                     ALGVRRPGVLGIYKALGAELDPRPLGDLLIKQGWRLALPAVESEDG"
     gene            complement(644..970)
                     /locus_tag="PLO65_08635"
     CDS             complement(644..970)
                     /locus_tag="PLO65_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRD28352.1"
                     /translation="MAQVTVQVNGKPYSVGCEDGQERHLVEIARLFDEQVRQVSQDVG
                     NLGETRLFLMGALLLADEMADLKLRLQHIQGELARTQTEQAKIEVKAVAALENAAKRI
                     EKLAAG"
     gene            complement(976..1266)
                     /locus_tag="PLO65_08640"
     CDS             complement(976..1266)
                     /locus_tag="PLO65_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HRD28353.1"
                     /translation="MTGEAEGSALDLAVRRLERAMAMLESRMSDRMAEAGAEAGGLFD
                     QDRARLAAELDAARARERALEEAGAQASQALGRAIEEIRAALGDEPANGTEG"
     gene            1392..3356
                     /gene="tkt"
                     /locus_tag="PLO65_08645"
     CDS             1392..3356
                     /gene="tkt"
                     /locus_tag="PLO65_08645"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519108.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRD28354.1"
                     /translation="MSVSPVRMADAIRVLSMDAVHAARSGHQGAPMGLADVATVLWTK
                     FLRYDASRPDWADRDRFVLSAGHASMLIYSLLHLSGFKSVSMDDIRAFRQWGSTTAGH
                     PEYGHTAGVETTTGPLGQGIATAVGMAMAERHMNARYGDDLVDHRTWVIAGDGCLMEG
                     VSHEAISIAGRLKLNRLTVLFDDNNTTIDGAATISETGDQMARFKAAGWAVKAVDGHD
                     HGKIAAALRWATKQDRPTMIAVRTRILKGAGDKEGDVHGHGYSLFDEEAIVARKAMGW
                     PLPPFELPDDIVAAWRKAGRKGGSQRRKWEARLKASDRRADFTRAMKGDLPADAFAGL
                     DAHIGKMLAEPVVNATRVHSGAALATLTPAIEDMVGGSADLTGSNNTYVKGTAPFDAP
                     DYAGRYVHYGVREHGMAAAMNGMALHGGVIPYSGTFLAFADYSRAAIRLGALMGVRVI
                     HVMTHDSIGLGEDGPTHQPVEHVASLRAMPNLLVFRPADAVEAAECWRLALEAKTTPS
                     VMALSRQKTGVVRTTGGNLSARGAYEAAAADGAARVTIFASGSEVPIALAARDLLQAD
                     GVPTRVVSTPCWELFEQQTARYRADTIGKAPVRVAVEAGVKFGWERFIGEDGVFVGMA
                     GFGASAPYERLYKEFGITAEAVAAAAKAKL"
     gene            3531..4727
                     /gene="purT"
                     /locus_tag="PLO65_08650"
     CDS             3531..4727
                     /gene="purT"
                     /locus_tag="PLO65_08650"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRD28355.1"
                     /translation="MKLGTPLSPSATKVMLLGCGELGKEVAIELQRLGVEVIGVDRYA
                     DAPAMQVAHRSHVIAMTDPAALRAVIEAERPHLVVPEIEAIATDELARIEAEGLATVI
                     PTARAAQLTMNREGIRRLAAEELGLPTSPYAFADSREALEAGAQAVGYPCFVKPVMSS
                     SGKGQSYVECAAGIGAAWDYALSAGRVETALVIVEGRIDFDFEITLLTVRSLGADGQI
                     RTSFCAPVGHRQVKGDYVESWQPQAMSAAALASAQDIAGKVTAALGGRGLFGVELFVK
                     GDQVWFSEVSPRPHDTGLVTLATQHLSEFALHARAILGLPVDVSLREPGASAVIYGGM
                     EAVGVAYEGVAEALSGPGVDLRLFGKPEAFERRRMGVALARAADTDTARANAAAAAGK
                     VKVVRA"
     gene            5019..>5488
                     /locus_tag="PLO65_08655"
     CDS             5019..>5488
                     /locus_tag="PLO65_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HRD28356.1"
                     /translation="MTLRVAINGFGRIGRLVLRSIAEHNRTDIEVVAINDLGPVETNA
                     HLFRYDSVHGRFPGTVTTGEDWIDIGRGKIKVTAIRNPAELPHKDLGVDIAFECTGLF
                     TSKEKASAHLAAGAKRVLVSAPAEGADKTIVFKVNHETLTADDIVVSNGSCTTNA"
BASE COUNT          845 a         1966 c         1875 g          802 t
ORIGIN      
        1 gcccgtcctc gctctcgacg gccggcaggg ccagccgcca gccctgcttg atcagcaggt
       61 cgccgagcgg gcgggggtcg agctcggcgc ccagggcctt gtagatcccc agcaccccgg
      121 ggcggcgtac gcccagcgcc gccagcatct cgccggcccg gtcggccgcc atccagtcgg
      181 cctcgggatg ctcgacgagc aggcccttgc gccggttgcg catctccatg cggagggtgg
      241 ctttctgggc ggcgagggcg gggtcggtca ctttggtgcg tccttcgaca cggcccttcg
      301 ggcctgctca ggatgacgta gctctgtgca cgtcatggtg agcagcagcc gcagactgcg
      361 tgtcgaacca cgcaccatgc aatgcggaaa tagggtggcg gcgccgcgag agccgtgaag
      421 gtcggttcta tcccctcgac ctggttgacc aggtgggcgc cgagtgcccg accccacggg
      481 gaaggacagg gtcagctccc tcagagagaa ttaaggtcgc tgggatgtgt tgccttcgac
      541 gagagccgca gcaggacccg ccgccggtca aggtagggct tcgagcgggc aaagggaagg
      601 ccggccctgg ataccttctc ccgcaagggg agaaggagca aaactacccc gccgccagct
      661 tctcgatgcg cttggcggcg ttctccagcg ccgccacggc cttgacctcg atcttggcct
      721 gttcggtctg ggtccgggcc agttcgccct ggatgtgctg cagccgcagc ttgaggtcgg
      781 ccatctcgtc ggccagcagc agggcgccca tcaggaacag ccgcgtctcg ccgagattac
      841 cgacgtcctg gctgacctgg cggacctgtt cgtcgaacag ccgggcgatc tcgaccagat
      901 gacgttcctg gccgtcctcg cagccgacgc tgtagggctt gccattaacc tgcacggtga
      961 cctgggccat ggcggtcagc cttccgttcc gttggcgggc tcatcgccca gcgcggcgcg
     1021 tatctcctcg atggcgcgac ccagcgcctg cgacgcctgg gccccggcct cttccagcgc
     1081 ccgctcgcgg gcccgggcgg cgtccagttc ggcggccagg cgtgcgcggt cctggtcgaa
     1141 cagaccgccg gcctcggcgc ccgcctcggc catcctgtcg gacatgcggc tctcgagcat
     1201 cgccatcgcg cgctccagcc ggcgcacggc cagatcgagg gcgcttccct cggcctctcc
     1261 cgtcatcatc gcggctccct gatcgaagtc ctttgtatag accgcgccgg ggcgcgacag
     1321 gaaggcgggt tgaaacgatc ctttccacgt gcgaacccgc cccagtccca cgcgagtcga
     1381 gagagccgaa catgtccgtc agcccggtca ggatggccga cgccatccgc gttctttcca
     1441 tggacgccgt ccatgccgcc aggtccggcc accagggcgc cccgatgggc ctggccgacg
     1501 tggccacggt gctgtggacg aagttcctgc gctacgacgc cagccggccg gactgggccg
     1561 accgcgaccg gttcgtgctg tcggccggcc acgcctcgat gctgatctac agcctgctgc
     1621 acctgtcggg cttcaagtcg gtcagcatgg atgacatccg cgccttccgt cagtggggca
     1681 gcaccacggc cgggcatccc gaatacgggc acaccgccgg ggtcgagacc accaccggcc
     1741 cgctgggcca gggcatcgcc acggccgtcg gcatggccat ggccgagcgc cacatgaacg
     1801 cccgctacgg cgacgacctg gtcgaccacc gcacctgggt catcgccggc gatggctgcc
     1861 tgatggaggg cgtcagccac gaggccatct cgatcgccgg ccggctgaag ctgaacaggc
     1921 tgaccgtgct gttcgacgac aacaacacca ccatcgacgg ggcggccacc atctccgaga
     1981 ccggcgacca gatggcccgc ttcaaggcgg ccggctgggc ggtcaaggcg gtcgacggcc
     2041 acgaccacgg caagatcgcc gcggccctgc gctgggcgac gaaacaggac cggccgacga
     2101 tgatcgcggt ccgcacccgg atcctcaagg gcgccggtga caaggaaggc gacgtccacg
     2161 gccacggcta ttcgctgttc gacgaggagg ccatcgtcgc gcgcaaggcg atgggctggc
     2221 cgcttccgcc gttcgaactg cccgacgaca tcgtcgccgc ctggcgcaag gccggccgca
     2281 agggcggctc gcaacgccgc aagtgggagg cccgcctcaa ggccagcgac cgacgcgccg
     2341 acttcacgcg ggccatgaag ggcgatctgc cggccgacgc cttcgccggc ctcgacgccc
     2401 atatcggcaa gatgctggcc gagccggtgg tcaatgccac gcgcgtccat tccggcgcgg
     2461 ccctcgccac gctgaccccg gcgatcgagg acatggtcgg cggctcggcc gacctgaccg
     2521 gttcgaacaa cacctacgtc aagggcacgg ccccgttcga cgcgcccgac tacgccggcc
     2581 gctacgtcca ctacggcgtg cgcgagcacg gcatggccgc ggcgatgaac ggcatggcgc
     2641 tgcatggcgg ggtgatcccc tacagcggca ccttcctggc cttcgccgac tacagccgcg
     2701 cggccatccg cctgggcgcc ctgatgggcg tgcgggtgat ccatgtgatg acccacgact
     2761 cgatcggcct gggcgaggac ggcccgaccc accagccggt cgagcatgtc gccagcctgc
     2821 gggccatgcc caacctgctg gtgttccgcc cggccgacgc ggtcgaggcc gccgagtgct
     2881 ggcgtctggc gctcgaggcg aaaaccaccc cgtcggtgat ggccctgtcg cggcagaaga
     2941 ccggcgtggt ccgcaccacg ggcggcaacc tgtcggccag gggcgcctat gaggccgccg
     3001 ccgccgacgg cgcggcccgg gtgacgatct tcgcgtccgg ctcggaggtt ccgatcgcgc
     3061 tcgccgcccg tgacctgctg caggccgacg gcgtgccgac ccgggtggtc tcgaccccct
     3121 gctgggagct gttcgagcag cagacggcca ggtaccgcgc cgacaccatc ggcaaggccc
     3181 cggtgcgggt ggctgtcgag gccggcgtga agttcggctg ggagcggttc atcggcgagg
     3241 acggcgtatt tgtcggcatg gccggcttcg gcgcctcggc gccctacgag cgactgtaca
     3301 aggaattcgg catcaccgcc gaggccgtcg cggcggcggc gaaggcgaag ctctgacacc
     3361 ctgcgtcctt cggcacgccg cttcgcgctt gttcaggatg acgtagactt tccacgtcat
     3421 ggtgagcagc gcccgcaggg cgcgtgtcga accatgcaca ctggcagttt gaggggacag
     3481 cttaagctgt ccccggttac gcccccgatt acgccaacag ggatcaagtc atgaaactcg
     3541 gcacccccct cagcccgtcg gccacgaagg tcatgctcct cggctgcggg gagctcggca
     3601 aggaagtggc catcgagctg cagcggctgg gcgtcgaggt gatcggtgtc gaccgctacg
     3661 ccgacgcccc ggccatgcag gtggcccacc gcagccatgt catcgccatg accgaccccg
     3721 ccgccctgcg cgcggtgatc gaggccgagc ggccacacct ggtcgtgccc gagatcgagg
     3781 ccatcgccac cgacgagctg gcccgcatcg aggccgaggg cctggcgacg gtgatcccca
     3841 cggcccgggc cgcccagctg acgatgaacc gtgagggcat ccgccggctg gcggccgaag
     3901 agctcggcct gccgaccagc ccctacgcct ttgccgacag ccgcgaggcg ctggaggccg
     3961 gcgcgcaggc ggtcggctat ccctgcttcg tcaagccggt gatgagcagt tccggcaagg
     4021 gccagtccta cgtcgagtgc gcggccggca tcggcgcggc ctgggactac gccctgtcgg
     4081 ccgggcgggt cgagacggcg ctggtgatcg tcgaggggcg catcgacttc gacttcgaga
     4141 tcaccctgct gacggtccgc tcgctcggcg ccgacggcca gatccggacc tccttctgcg
     4201 cgccggtcgg ccaccgccag gtcaagggcg actatgtcga gagctggcag ccgcaggcga
     4261 tgagcgccgc cgccctggcc tcggcccagg acatcgccgg caaggtcacc gccgcgctcg
     4321 gcggccgggg cctgttcggc gtcgagctgt tcgtgaaggg cgaccaggtg tggttctccg
     4381 aggtcagccc gcgaccgcac gacacgggcc tggtcaccct ggccacccag catctgagcg
     4441 agttcgcgct gcacgcccgc gccatcctcg gcctgcccgt cgacgtcagc ctgcgcgaac
     4501 cgggcgccag cgcggtgatc tatggcggca tggaggctgt cggggtcgcc tatgagggcg
     4561 tggccgaggc gctgtccggc ccgggcgtcg acctgcgcct gttcggcaag cccgaggcct
     4621 tcgagcgccg ccgcatgggc gtggcgctgg cgagggccgc cgacaccgac acggcgcgag
     4681 cgaacgcggc ggcggcggcg ggcaaggtga aggtggttcg tgcctgaaca atcctcccct
     4741 cgtcagaggg gaggggaacc acccggagga tggtggaggg gcggaaccgt gctctccgga
     4801 cggaccggaa ttcaggatgg ggccgctcgc atcagcactg cccctccacc gcgctccgcg
     4861 cggtccccct ccccgcttcg cggagaggaa tggtgaagat cgtcacaaac cttcaccgga
     4921 tgtgaccgtc gcaaatcctc tcgcggggtt gactccgcgg cccctgaccg caaaacccgc
     4981 gcccaatcag gaaccttgcc gccgggagcg cttcactcat gacccttcgc gtcgccatca
     5041 acggatttgg ccggatcggc cgtctggttc tgcgctcgat cgccgagcac aaccgcacgg
     5101 acatcgaggt cgtggcgatc aacgacctgg gtccggtgga aaccaacgcc cacctgttcc
     5161 gctatgacag cgttcatggc cgcttccccg gcacggtgac gaccggcgag gactggatcg
     5221 acatcggccg cggcaagatc aaggtcacgg cgatccgcaa tccggccgag ctgccgcaca
     5281 aggacttggg cgtcgacatc gccttcgaat gcacgggcct gttcacctcg aaggaaaagg
     5341 cctccgccca cctggccgcc ggcgcgaagc gggtgctggt ttcggccccg gccgagggcg
     5401 ccgacaagac catcgtcttc aaggtcaacc acgagaccct gaccgccgac gacatcgtcg
     5461 tctccaacgg cagctgcacc accaacgc
//
LOCUS       DAORNX010000170         5471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11050_length_5471_cov_6.929099, whole genome shotgun sequence.
ACCESSION   DAORNX010000170 DAORNX010000000
VERSION     DAORNX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11050_length_5471_cov_6.929099"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLO65_08660"
     CDS             complement(<1..474)
                     /locus_tag="PLO65_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRD28357.1"
                     /translation="MPDSGARPRDPPGVVHRALKHDSAERHVSGRALYIDDIPEPRDL
                     LHVALGMSARAHARIVSLDLTPVRAAPGVVDVVCAQDIPGTNDVSPVAGDDPLFADGE
                     VLYVGQSLFAVAAETVDQARAAARLAVVTYEDLPADLTIADARAAGSTLEPAQTMR"
     gene            complement(467..1879)
                     /gene="xdhA"
                     /locus_tag="PLO65_08665"
     CDS             complement(467..1879)
                     /gene="xdhA"
                     /locus_tag="PLO65_08665"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRD28358.1"
                     /translation="MRDVVRFVLDGEGRELRDIDPTTTVLNLLRRDLRRTGTKEGCAE
                     GDCGACTVALGELDGDRVKWRAVNACILFAPMLDGRALKTVESLGGDHPVQTALVEHH
                     GSQCGFCTPGFVMSLWARGREDNPPPVADAIAGNLCRCTGYGPILAAGEAAGTRAEDD
                     PVAALQALSGDEPLSLTHDGRRWFAPRALSDLAGLVEAHPDAVILAGATDVGLWVTKQ
                     HRDLQTVISIGDVAELKTITETSGGVRIGAGVRYSDALATLAGLHRDLGELVRRLGSV
                     QVRNSGTIGGNIANGSPIGDMPPALIALGATLVLRKGGERRETPLEDYFLGYGKQDRR
                     PGEFVEAVIVPRPREGMLYRVFKLSKRFDQDISAVCAGFALTIKDDVVQNARIAFGGM
                     AATPKRALACEAALTGQPWTGATIERATQALEREFAPIDDVRASAVYRMTAAQNLLRK
                     LFAETGGATIDVLAAEPVDA"
     gene            complement(1882..2223)
                     /gene="uraH"
                     /locus_tag="PLO65_08670"
     CDS             complement(1882..2223)
                     /gene="uraH"
                     /locus_tag="PLO65_08670"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRD28359.1"
                     /translation="MTGLTTHVLDTTHGRPAAGVALRLFRDGQLMVETRTNADGRCDA
                     PLLTGETIHRGHYRLEFAIGAYFRGRGLDLPEPSFLEIVAIEFGIAHPADHYHVPLLV
                     SPYGYSTYRGS"
     gene            2359..3267
                     /gene="puuE"
                     /locus_tag="PLO65_08675"
     CDS             2359..3267
                     /gene="puuE"
                     /locus_tag="PLO65_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HRD28360.1"
                     /translation="MSDGYPRDLIGYGPNPPHADWPGGARIAIQVVLNYEEGGEAAVL
                     HGDAAAETFLSDMIFPAPVQGARHMSMESLYEYGARAGVWRLLRLFERYDLPLTVFGV
                     AMAMERNPAVVEAFLQAGHEIASHGWRWINYQAVPEAVEREHMARAIEVHARLTGERP
                     LGWYAGRTSPNTRRLAAEEGGFLYDADDYSDDLPFWSTQTATPHLIVPYTLEANDMRF
                     SGTGLNTGEQFFSYLRDAFDVLYAEGEERPRMMSIGLHCRLAGRPGKMAGLERFLKHA
                     LSHQDVWFARRIDIARHWRERHPHAR"
     gene            3257..3739
                     /gene="uraD"
                     /locus_tag="PLO65_08680"
     CDS             3257..3739
                     /gene="uraD"
                     /locus_tag="PLO65_08680"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HRD28361.1"
                     /translation="MPGREGFIERFGGVYEDSPWVAEAVWPGAGALRSARSMATAMAT
                     VVDSAPRERKLALIRAHPQLAGRARMADASVREQSGAGLDQCTPDEFAAFQRLNAAYN
                     DRFGFPFIIAVKGLGRRDILDAFESRLANDPDAEFAEALRQIHRIAAFRLAELIEDAP
                     "
     gene            3736..4722
                     /gene="alc"
                     /locus_tag="PLO65_08685"
     CDS             3736..4722
                     /gene="alc"
                     /locus_tag="PLO65_08685"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HRD28362.1"
                     /translation="MSHPFAGLIDLAQPRLGSAVVYATDDFFADKARLIDPAEPVFIP
                     GKYDDNGKWMDGWESRRKRVAGHDWAIVRLGAPGEIAGFEVDTRHFTGNYPPRASIEA
                     CLSDAGIPPDDAEWTTLVPVMDLNGNDRRLVKAEARGVWTHVRLSIYPDGGVARLRVF
                     GTIHKDWREVPPDAVLDLLAMEHGGRGIIANDEHFGRVENLTAPGRGRDMGDGWETRR
                     RREPGHDWAILELGHAGTIEEITVDTAHFKGNYPDRCAIQASATATGSPADIARQAET
                     WPVLLPETKLQPDHVHVFTREILPHAPVRFVRLNIYPDGGVSRLRLKGRPAY"
     gene            complement(4716..5060)
                     /locus_tag="PLO65_08690"
     CDS             complement(4716..5060)
                     /locus_tag="PLO65_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28363.1"
                     /translation="MKTLAALALAAALGLAGPALAASPEEAEATLRTTISQIQAGTPD
                     YDAMVPALADAVKRNPDAPAQLAALGPVTTVVATSQADPFTYDVTFQNGAVLHWTLSI
                     NADGKIQGLLVQ"
     gene            5192..>5471
                     /locus_tag="PLO65_08695"
     CDS             5192..>5471
                     /locus_tag="PLO65_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD28364.1"
                     /translation="MTQETAQASGTLATALAHAAGLLTQDPALAEAQAREILRVLPTH
                     PQAILLLAAALRRQGGAEAALTVLEPVIRAEPNWPPVHQEAGLALADLG"
BASE COUNT          816 a         1902 c         1970 g          783 t
ORIGIN      
        1 gcgcatggtc tgggccggtt caagggtcga tccggccgcg cgggcgtcgg cgatggtcag
       61 gtcggccggc aggtcctcgt aggtcacgac cgccagccgg gcggcggcgc gggcttggtc
      121 gacggtctcg gcagccacgg cgaacagcga ctggccgacg tagagaacct ccccgtcggc
      181 gaacagggga tcgtccccgg ccaccgggct gacgtcgttg gtcccgggga tgtcctgcgc
      241 gcagaccacg tcgaccacgc ccggcgcggc gcggacgggg gtcaggtcca gcgagacgat
      301 gcgggcatgg gcccgcgcgc tcatgcccag cgcaacgtgc agcaggtccc gcggctcggg
      361 aatgtcgtcg atgtagaggg cgcggccgga gacgtggcgc tcggcgctgt catgcttcaa
      421 cgcccggtgg acgacgccgg gcgggtcgcg ggggcgggcg ccggagtcag gcatcgaccg
      481 gctccgcggc cagcacgtcg atcgtcgcgc cgccggtttc ggcgaacagc ttgcgcagca
      541 ggttctgcgc cgccgtcatc cgatagacgg ccgaggcgcg aacgtcgtcg atcggcgcga
      601 actcgcgctc cagcgcctgg gtggcccgtt cgatggtggc cccggtccag ggttgaccgg
      661 tgagcgccgc ctcgcaggcc agggcccgtt tcggcgtcgc cgccatgccg ccgaaggcga
      721 tgcgggcatt ctgtacgacg tcgtccttta tcgtcagggc gaagccggcg cagacggcgg
      781 agatgtcctg gtcgaaccgt ttggacagct tgaacacgcg atagagcatg ccttcgcggg
      841 ggcgcgggac gatcaccgcc tcgacgaact cgcccggccg ccggtcctgc ttgccgtagc
      901 cgaggaagta gtcctccagc ggcgtctcgc ggcgctcacc gcccttgcgc aacaccaggg
      961 tggcgcccag cgcgatcagg gccggcggca tgtcgccgat cggcgagccg ttggcgatgt
     1021 tgccgccgat cgtgccgctg ttgcgcacct gcaccgagcc cagccggcgg accagttcgc
     1081 cgaggtcgcg gtgcagtccg gcgagggtcg ccagcgcgtc gctgtagcgg acgcctgcgc
     1141 cgatgcggac accgccggat gtttccgtga tggtcttcag ctcggcgacg tcaccgatcg
     1201 agatgacggt ctgcaggtcg cggtgttgct tggtcaccca caggccgaca tcggtcgcgc
     1261 cggccaggat caccgcgtcg ggatgggcct cgacgagacc cgccagatcg ctgagcgccc
     1321 tgggcgcgaa ccagcggcgg ccgtcatggg tcagagacaa cggctcgtcg ccggacagcg
     1381 cctgaagcgc cgcgaccggg tcgtcttccg cccgggttcc ggccgcctcg ccggccgcca
     1441 ggatcgggcc gtagcccgtg cagcggcaca gattgccggc gatcgcgtcc gccaccggcg
     1501 gcggattatc ctcgcgcccc ctcgcccaaa gggacatcac gaaccccggg gtgcagaagc
     1561 cgcactgcga gccgtggtgc tcgaccaggg ccgtctgcac cggatggtcg ccgccgagac
     1621 tctcgaccgt cttcagcgcc cggccgtcga gcatcggcgc gaacaggatg caggcgttga
     1681 cggcccgcca tttcacgcgg tcgccgtcca gttcgccgag cgccacggtg caggcgccgc
     1741 agtcgccctc ggcgcaaccc tccttggtcc cggtccgacg caggtcacga cgcagcaggt
     1801 tgagcaccgt cgtcgtcggg tcgatgtcgc gcagctcccg cccctcgcca tcgagcacga
     1861 atcggacgac gtcgcgcacg ctcagctccc ccggtaggtc gagtagccgt agggcgaaac
     1921 cagcagcggc acatgatagt ggtcggccgg gtgggcgatg ccgaactcga tcgccacgat
     1981 ttcgaggaac gacggttcgg gcaggtcgag acccctgccg cgaaaatagg ccccgatcgc
     2041 gaactcaagg cggtagtggc cgcgatggat cgtctcgccg gtcagcagcg gcgcgtcgca
     2101 gcggccatcg gcgttggtgc gggtctcgac catcagctgg ccgtcgcgga acaggcgcag
     2161 ggcgacgccc gcggccggcc ggccatgggt cgtgtccaga acgtgggtcg tcagtccggt
     2221 catggtcgct cctgggccgc agggcgcggg ggagccggtc agaatctccc ccgatcgcgt
     2281 cgaatgcaac cttcaccgtc ggacaaggtc tcgtaaacct tgacgatccg cctgcgacgg
     2341 gcctgctatg gccgtcccat gagcgacggc tatcccagag acctgatcgg ctacggcccc
     2401 aatccgcctc acgcggactg gccgggcggc gcgcgcatcg cgatccaggt cgtgctcaac
     2461 tacgaggagg gcggcgaggc cgccgtgctg cacggcgacg ccgcggccga gaccttcctg
     2521 tccgacatga tcttccccgc gccggtccag ggcgcgcggc acatgagcat ggagtcgctc
     2581 tacgaatacg gcgcgcgggc cggggtgtgg cggctgctgc ggctgttcga gcgctacgac
     2641 ctgccgctga cggtgtttgg cgtggccatg gcgatggaac gcaacccggc cgtggtcgag
     2701 gccttcctgc aggcggggca cgagatcgcc agtcacggct ggcgctggat caactaccag
     2761 gccgtccccg aggcggtcga acgcgagcac atggcccggg cgatcgaggt ccacgcccgc
     2821 ctgaccggcg agcggccgct gggctggtac gcgggccgca ccagtcccaa cacccgccgg
     2881 ctggcggccg aggagggcgg cttcctctat gacgccgacg actattcgga cgacctgccg
     2941 ttctggtcga cgcagacggc cacgccgcac ctgatcgtgc cctacacgct ggaggccaac
     3001 gacatgcgct tttccggcac gggactgaac accggggagc agttcttcag ctacctgcgc
     3061 gacgccttcg acgtgctcta cgccgagggt gaggagcggc cgcggatgat gtcgatcggc
     3121 ctgcactgcc ggctggccgg gcgacccggc aagatggccg gactggagcg gttcctgaaa
     3181 catgccctgt cgcaccagga tgtctggttc gcccggcgca tcgacatcgc ccgtcactgg
     3241 cgggagcggc atccgcatgc ccggtaggga aggcttcatc gagcgcttcg gcggggtcta
     3301 cgaggattcg ccgtgggtcg ccgaggcggt ctggcccggc gcgggcgcgc tgcggtcggc
     3361 gcggtcgatg gccacggcga tggccacggt ggtcgactcg gcgccgcgcg agcggaagct
     3421 ggccctgatc cgcgcccacc cccagctggc cgggcgggct cgcatggcgg acgcctcggt
     3481 gcgcgagcag tccggggccg gcctggacca gtgcacgccc gacgagttcg ccgccttcca
     3541 gcggctcaac gcggcctaca acgaccgctt cggctttccc ttcatcatcg cggtgaaggg
     3601 cctgggccgt cgcgacatcc tcgacgcgtt cgaatcccgt ctggccaacg atccagacgc
     3661 ggagttcgcc gaggccctgc gccagatcca ccgtatcgcc gccttccggc tggccgagct
     3721 gatcgaggac gcaccgtgag ccatcccttc gccggcctga tcgacctggc ccagccgcgg
     3781 ctgggaagcg cggtggtcta cgccaccgac gacttcttcg ccgacaaggc ccggctgatc
     3841 gacccggcgg agccggtgtt catccccggc aagtacgacg acaacggcaa atggatggac
     3901 ggctgggaga gtcgccgcaa gcgcgtcgcc ggccacgact gggcgatcgt cagactgggg
     3961 gcgccaggcg agatcgccgg cttcgaggtc gacactcgcc atttcaccgg aaactacccg
     4021 ccgagggcgt cgatcgaggc ctgcctgtcg gacgccggaa tcccgcccga cgacgcggaa
     4081 tggacgacgc tggtccccgt catggatctg aacggaaatg accgacgcct ggtgaaggcc
     4141 gaggcccgcg gcgtctggac ccacgtgcgg ctgagcattt atcccgacgg tggcgtggcg
     4201 cggctgcggg tgttcgggac catccacaag gactggcgcg aggtcccgcc ggacgccgtg
     4261 ctcgacctgc tggcgatgga gcacggcggt cgcgggatca tcgccaacga cgagcacttc
     4321 ggccgggtcg agaacctcac cgcgccgggc cggggccggg acatgggcga cggatgggaa
     4381 acccgtcgcc gccgcgaacc gggccacgac tgggcgatcc tcgagctcgg ccatgccgga
     4441 acgatcgagg agatcaccgt cgacaccgcc cacttcaagg gcaactaccc cgaccgctgc
     4501 gcgatccagg cgtcggccac ggccacaggc tcgccggcgg acatcgcccg gcaggcggag
     4561 acctggccgg tgctgctgcc cgagacgaag ctccagcccg accacgttca tgtcttcacg
     4621 cgcgagatcc tgccgcacgc accggtccgg ttcgtgcggc tgaacatcta ccccgacggc
     4681 ggcgtcagcc ggctgcggct caagggccgg ccggcctact gaaccagcag gccctggatc
     4741 ttgccgtcgg cgttgatcga cagggtccag tgcagcaccg cgccattctg gaaggtgacg
     4801 tcataggtga acgggtcggc ctggctggtg gcgacgaccg tggtcaccgg gcccagggcc
     4861 gccagctgcg ccggagcgtc ggggttccgt ttcaccgcgt cggcgagcgc cggaaccatg
     4921 gcgtcatagt ccggcgttcc ggcctggatc tgggagatgg tggtccgcaa ggtcgcttcc
     4981 gcctcttccg gcgaggcggc gagcgccggg ccggcgagac caagggcggc ggcgagggcg
     5041 agggcggcga gggtcttcat gagggcgctc ctgaacagcg atgaacgcca taggccagcc
     5101 gggtgaccgg cgcagcttac cttttggtca tgcggggaag gctcaagatg cgggtcgaca
     5161 gggacgctga acctgtgcac ggcttggcgc gatgacgcag gaaaccgccc aagcctcagg
     5221 cacgctggcc acagccctgg cccatgccgc cggccttctg acccaggatc cggcgctggc
     5281 cgaggcccag gcgcgcgaaa tcctgcgggt cctgccgacg cacccgcagg cgatcctgct
     5341 gctggcggcc gcgttgcggc gtcagggcgg cgccgaggcg gccctgaccg tgctcgagcc
     5401 ggtgatccgg gccgagccga actggccgcc cgtacatcag gaggccggtc tggcgctggc
     5461 cgatctcggc c
//
LOCUS       DAORNX010000171         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11080_length_5461_cov_6.246393, whole genome shotgun sequence.
ACCESSION   DAORNX010000171 DAORNX010000000
VERSION     DAORNX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11080_length_5461_cov_6.246393"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLO65_08700"
     CDS             <1..396
                     /locus_tag="PLO65_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28365.1"
                     /translation="AAAGAVRSQQRPDCVRVPAVVDRASMPLALGGLPAILVAQRGVR
                     TPSARAPARHHLAAWWRKVMSDGSEQAIDDEELRAILLHNAAEELEHAAMALEWLRRN
                     DPQVDENLREYLFKDGSITGHEEQATGKA"
     gene            459..>867
                     /locus_tag="PLO65_08705"
     CDS             459..>867
                     /locus_tag="PLO65_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRD28366.1"
                     /translation="MVEIAPCEAGSRLDKTLADRLPELSRGRVQALMAAGMVTRDGAA
                     VTDASGKAGAGLYRVLIPPPESTQVVAEAIPLTVLFEDAHLIVVDKPAGMAAHPGPGT
                     PGGTLVNALLHHCGASLSGIGGVARPGIVHRLDK"
     assembly_gap    868..933
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <934..1415
                     /locus_tag="PLO65_08710"
     CDS             <934..1415
                     /locus_tag="PLO65_08710"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRD28367.1"
                     /translation="SHDIDRLYIALTRGAPSPAKGTITTQLGRSQHDRKKMAILRTGG
                     REAITHYRVERAFGPQARPLAARVSCRLETGRTHQIRVHLASKGAPCLGDPTYGSGPP
                     APVVREAMAVAGLRRQALHAAVLGFVHPVTGETMRFETPLPEDMRRLEEALSRFPSH"
     gene            1684..2571
                     /gene="rpoH"
                     /locus_tag="PLO65_08715"
     CDS             1684..2571
                     /gene="rpoH"
                     /locus_tag="PLO65_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRD28368.1"
                     /translation="MAATSLAVMSPEGGLSRYLTEIRKFPMLAKDEEFMLAKAWKEHE
                     DSEAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPEKGFRLAT
                     YAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKAEISAFEEGDLRPEHVKT
                     IATKLGVLDEEVISMNRRLSGGDASLNAPMRADGESEWQDWLADDTAVSQETQVAEAE
                     ESSLRMGLLQEAMAELSDRERHILTERRLKDDPTTLEELAAEYGVSRERVRQIEVRAF
                     EKLQKTMRAAALERNLVEA"
     gene            complement(2572..3369)
                     /locus_tag="PLO65_08720"
     CDS             complement(2572..3369)
                     /locus_tag="PLO65_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28369.1"
                     /translation="MFTADDKLQQRLTPHLQRVLIVDPQPAAVKLLGELLRSVTPGQI
                     ATAASDQKAIQLAQAVDPQIIFIEHSGPSLDGARLARAIRRSDLLCRKAPIIMVTGEA
                     TAQAILGARDAGVHEFLRKPFTIKDLLRRLEAVALKPRDWVEAVQYVGPDRRRFNSGE
                     YAGPRKRKSDARKTPDQARVLQALKIVRAAAGALDSDWAQARRALLAQAQDLQKAAVA
                     VGDMKLMTAAVDLGRQLSAEGPVNRADLDGAIAALVALMPRDEALAA"
     gene            complement(3447..4733)
                     /locus_tag="PLO65_08725"
     CDS             complement(3447..4733)
                     /locus_tag="PLO65_08725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRD28370.1"
                     /translation="MANVTVVGAQWGDEGKGKIVDWLSNRADVVVRFQGGHNAGHTLV
                     VDGKVYKLALLPSGVVQGKLSVIGNGVVVDPWHLLSEISKIADQGVAITPDLLVLADN
                     ACLILPVHRDLDQAREAASTQKIGTTGRGIGPAYEDKVGRRAIRVADLADPDALEAKI
                     DRLLSHHGALRRGLGLPEADGKALLAELLAIAPKILPYAQPAWRLLDQAYKAGRRILF
                     EGAQGALLDVDHGTYPFVTSSNTVAGQAAAGSGMGPRGPGYVLGIVKAYTTRVGEGPF
                     AAELDDEVGRHLATVGREVGVNTGRPRRCGWFDAVLVRQSVAINGIAGIALTKLDVLD
                     GLRTLKICVGYEINGKTFDYLPAGMCDQAAARPIYEEMEGWIDSTAGARSFKDLNASA
                     IKYVRRIEELIGAPVALLSTSPERDDTILMRDPFQG"
     gene            complement(4885..>5085)
                     /locus_tag="PLO65_08730"
     CDS             complement(4885..>5085)
                     /locus_tag="PLO65_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadD"
                     /protein_id="HRD28371.1"
                     /translation="RADARVRQNLALVIGLQGRLPEAEQLVRQDLPPDQAANNIAWLK
                     AATARAPTGEGRSWSAVTGGGS"
     assembly_gap    5086..5255
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5256..>5461)
                     /locus_tag="PLO65_08735"
     CDS             complement(<5256..>5461)
                     /locus_tag="PLO65_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein TadD"
                     /protein_id="HRD28372.1"
                     /translation="VALRQLGRYPEAADAAGQVLVIEPDNTEALLEAARAQIGRGQGF
                     YAIEPARHAQALAPRDWRPVSLLA"
BASE COUNT          798 a         1796 c         1873 g          758 t
ORIGIN      
        1 gctgccgcgg gcgccgtgcg cagccagcag cgtccggatt gcgttcgcgt tccggcggtt
       61 gtggatcgag cgtcgatgcc gttagcactc ggtgggttgc cggcgatcct tgttgcgcag
      121 cggggcgtgc gaaccccgtc ggcgcgtgcg ccagctagac atcatctcgc cgcgtggtgg
      181 cgcaaggtca tgtcggacgg gtcggagcag gccatcgacg acgaggaact gcgggccatc
      241 ctgctgcaca acgcggccga ggagctcgag cacgccgcca tggcgctgga atggctgcgc
      301 cgcaacgatc cgcaggtcga cgagaacctg cgcgaatacc tgttcaagga cggctcgatc
      361 acgggccacg aggaacaggc gaccggcaag gcgtgaccga cgccgccgac ctttccgagg
      421 acgacgaggc gcttgagccg gaccagcccg gcgagcgggt ggtcgagatc gcgccgtgcg
      481 aggccgggtc gcggctggac aagaccctgg ccgaccggtt gccggagctg tcgcgcggcc
      541 gggtgcaggc gctgatggcg gccgggatgg tcacgcgcga cggggcggca gtgaccgacg
      601 cctcgggcaa ggccggcgcg ggtctctacc gcgtcctgat cccgccgccg gagtcgacgc
      661 aggtcgtggc cgaggcgatc ccgctgacgg tgctgttcga ggacgcgcat ctgatcgtgg
      721 tcgacaagcc ggccgggatg gccgcccacc cgggtccggg aacgccgggc gggaccctgg
      781 tcaacgccct gctgcatcac tgcggggcca gtctgtcagg catcggcggc gtggcccggc
      841 ccggcatcgt ccaccggctc gacaaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccagcca cgacatcgac cggctgtaca
      961 tcgccctgac ccgcggcgcg ccgagcccgg cgaaaggcac gatcacgacg cagctgggcc
     1021 gcagccagca tgaccgcaag aagatggcca tcctcaggac cggcgggcgc gaggcgatca
     1081 cccattatcg cgtcgaacgc gccttcggac cgcaggcccg gccgctggcc gcgcgggtgt
     1141 cgtgccggct ggagaccggc cgcacccacc agatccgcgt gcatctggcc agcaagggcg
     1201 ccccgtgcct gggcgacccg acctacggct cgggcccgcc ggccccggtc gtccgcgagg
     1261 ccatggccgt cgccggcctt cgtcgacagg cgctgcacgc agccgtgttg gggttcgtgc
     1321 atccggtcac cggcgagacg atgcggttcg agacgcccct gccggaggat atgcggcggc
     1381 tggaggaggc gttgagccga tttccttctc actgagggag aaggagacgg cgccgaaatc
     1441 gcgggacttc cgggccgcat cctgccggga caggacgaga catttcggga cacgcccgcc
     1501 ccacggcctg tggatttttt ctgacaactc actgacacaa tctcgatacc cgagcgccac
     1561 agtcggggat tacaaattcc tcactggcct tcaaacggct ggaccggctt cctatctcgg
     1621 agctcaaggg ggcggatgat ggcgcttgcg ccgcgcccgc acgcagaaaa ggggatgtaa
     1681 ccaatggccg ccacgtctct cgccgtcatg tcgccggaag gcgggctgtc gcgttacctg
     1741 accgaaatcc gcaagttccc gatgctcgcc aaggacgagg agttcatgct cgccaaggcc
     1801 tggaaggagc acgaggattc cgaggccgcc cacaagctcg tcaccagcca cctgcgcctg
     1861 gtggccaaga tcgccatggg ttaccgcggc tacggcctgc cgatcgggga agtgatctcc
     1921 gaaggcaacg tcggcctgat gcaggcggtc aagaagttcg agccggagaa gggtttccgc
     1981 ctggccacct acgccatgtg gtggatccgc gcctcgatcc aggaatacat cctgcgcagc
     2041 tggtcgctcg tgaagatggg caccacggcg gcgcagaaga agctgttctt caacctgcgc
     2101 aaggccaagg ccgagatcag cgccttcgag gaaggcgacc tgcgtcccga gcatgtgaag
     2161 accatcgcga ccaagctggg cgtgctggac gaggaggtca tctcgatgaa ccgccgcctg
     2221 tccggcggcg acgcctcgct gaacgcgccg atgcgcgccg atggcgagag cgagtggcag
     2281 gactggctgg ccgacgacac cgccgtcagc caggagaccc aggtcgccga ggccgaggag
     2341 agcagcctgc gcatgggcct gctgcaggag gccatggccg agctgtcgga ccgcgagcgg
     2401 cacattctca ccgagcgccg gctgaaggac gacccgacca cgctcgagga actggccgcc
     2461 gaatacggcg tcagccgcga gcgggtgcgc cagatcgagg tgcgggcgtt cgagaagttg
     2521 cagaagacga tgcgcgcggc tgccctcgag cggaacctgg tcgaggcctg atcaggccgc
     2581 cagggcctcg tcccggggca tcagggccac cagggcggcg atggcgccgt cgaggtcggc
     2641 ccggttgacc ggtccctctg ccgacagctg acggccgaga tcaaccgccg ccgtcatcag
     2701 cttcatgtcg ccgaccgcga cggcggcctt ctgcagatcc tgggcctggg ccaacagcgc
     2761 ccggcgggcc tgggcccagt cgctgtccag cgccccggcc gcggcccgga cgatcttcag
     2821 cgcctgcagc actcgcgcct gatcgggcgt cttgcgcgcg tcggacttgc gcttgcgcgg
     2881 tccggcgtat tcgcccgagt tgaaccgccg ccggtcgggc ccgacgtact gcacggcctc
     2941 gacccagtcg cgcggcttga gcgccacggc ctcgagtcgc cgcagcaggt ccttgatggt
     3001 gaagggcttg cgcaggaact cgtgaacccc cgcgtcgcgg gcgccgagga tcgcctgggc
     3061 cgtcgcctcg ccggtgacca tgatgatcgg ggccttgcgg cacagcaggt cgctgcggcg
     3121 aatcgcgcga gccagcctcg ccccgtcgag gctggggccg gaatgttcga tgaagatgat
     3181 ctgcgggtcg accgcctggg ccagctgtat ggccttctgg tcgctggcgg cggtggcgat
     3241 ctggccgggc gtgaccgagc gcagcagttc gccgagcagc ttcaccgccg ccggctgcgg
     3301 atcgacgatc agcacccgct gcaggtgcgg cgtcagacgt tgctgcagct tgtcgtcggc
     3361 ggtgaacaag gtgtgctccg ggcccctctc caggggcgcc agaatgggac gaaccggtaa
     3421 agaacggctt gacggcccgg gctcaactat ccctgaaacg ggtcacgcat caggatggtg
     3481 tcgtcccgct ccggactcgt cgagagcagg gcgaccggcg cgccgatcag ttcctcgatc
     3541 cgccggacgt acttgatggc gctggcgttg aggtccttga acgagcgggc cccggcggtg
     3601 ctgtcgatcc agccctccat ctcctcgtag atcggcctgg ccgccgcctg gtcgcacatg
     3661 cccgcgggca ggtagtcgaa ggtcttgccg ttgatctcgt agccgacgca gatcttgagg
     3721 gtcctgagac cgtccagcac gtccagcttg gtcagggcga tgccggcgat gccgttgatg
     3781 gccaccgact ggcgcaccag caccgcatcg aaccagccgc agcgtcgcgg ccggccggtg
     3841 ttgaccccga cctcgcgacc gacggtcgcc aggtgcctgc cgacctcgtc gtcgagctcg
     3901 gccgcgaacg ggccctcgcc gacccgggtg gtgtaggcct tgacgatgcc cagcacatag
     3961 cccggaccgc gcggccccat gcccgacccg gccgccgcct ggccggccac cgtgttcgac
     4021 gaggtcacaa acggataggt gccgtggtcg acgtcgagca gggcgccctg ggcgccctcg
     4081 aacaggatgc ggcggccggc cttgtaggcc tggtcgagca ggcgccaggc cggctgggcg
     4141 tagggcagga tcttcggcgc gatcgccagc agctcggcca gcagggcctt gccgtcggcc
     4201 tcgggcaggc ccaggccccg gcgcagcgcg ccgtggtgcg acagcagccg gtcgatcttg
     4261 gcttcgagcg catcgggatc ggccagatcg gcgacgcgga tcgcccggcg tccgaccttg
     4321 tcctcgtagg ccggaccgat gccgcggccg gtggtgccga tcttctgggt cgaggcggcc
     4381 tcacgggcct ggtcaagatc acggtgcacg ggcaggatca ggcaggcgtt gtcggccagc
     4441 accagcaggt cgggcgtgat ggccacgccc tggtctgcga tcttggaaat ttccgacagc
     4501 aggtgccacg ggtcgacgac cacgccgttg ccgatcaccg acagcttgcc ctggaccacc
     4561 ccggacggca gcagggccag cttgtagacc ttgccgtcca cgaccagggt gtgaccggcg
     4621 ttgtggccgc cctggaaccg cacgaccacg tcggcgcggt tgctcagcca gtcgacgatc
     4681 ttgcccttgc cctcgtcacc ccactgggcg ccgacaacgg tcacattggc catggatacg
     4741 atcctcccgc ctgccgggtc gatccagcgc gcccttcgac aggctcctcg gcgcgtggac
     4801 tgggatcagt tgtagggccg cgcgaggcgg ccgcggcggc gtatagcgag cggggcgggc
     4861 gacgtccacc ccccgcggcg gtgatcagga gccgccgccg gtgaccgcgc tccaggacct
     4921 gccctcgccc gtgggggccc gggcggtggc ggccttgagc caggcgatgt tgttggccgc
     4981 ctggtccggc ggcaggtcct ggcggaccaa ctgctcggcc tcgggcaggc gcccctgcag
     5041 gccgatgacc agcgccaggt tctggcgcac ccgggcgtcg gcccgnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcc agcaacgaca cgggccgcca
     5281 gtcgcgcggg gcgagggcct gggcatgccg ggccggctcg atggcgtaga agccctgtcc
     5341 cctgccgatc tgggcgcggg cggcttcgag cagggcctcg gtattgtccg gctcgatcac
     5401 cagcacctgg ccggccgcgt cggcggcctc gggatagcgg cccagctggc gcagggcgac
     5461 a
//
LOCUS       DAORNX010000172         5391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11271_length_5391_cov_5.195465, whole genome shotgun sequence.
ACCESSION   DAORNX010000172 DAORNX010000000
VERSION     DAORNX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11271_length_5391_cov_5.195465"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLO65_08740"
     CDS             <1..423
                     /locus_tag="PLO65_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD28373.1"
                     /translation="DLAVAGESARFAVSGINVGLFCSTPAVPLSRAVGSKVALDMLFT
                     GRFISAAEALAHGLVSRVAPDADLDAAVDALTGEIAAKSPVAVRTGKAMFARQAALPL
                     DQAYAYAAGVMAGNMMAEDAGEGIDAFIGKRKPAWSGR"
     gene            complement(566..1180)
                     /locus_tag="PLO65_08745"
     CDS             complement(566..1180)
                     /locus_tag="PLO65_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRD28374.1"
                     /translation="MSEVQPAKPRPDLLKWAIRGAALIGVAAVLYVIVSASIKPKSDT
                     DLRQFAKGGLVKLVVPAAPTPAPTAGFTDADGKAMTLADFKGQIVVVNLWATWCAPCR
                     LEMPTLAKLQAAYQTQPVQVVAISVDRDADLNLARADIARNAPLKLYRDPGYKFAFGM
                     KPAAQGFPTTVIYDKQGRERARLSGGADWNGKEARALIETLLKE"
     gene            1181..2590
                     /gene="argH"
                     /locus_tag="PLO65_08750"
     CDS             1181..2590
                     /gene="argH"
                     /locus_tag="PLO65_08750"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRD28375.1"
                     /translation="MTDKSSNRAGAGQAMWGGRFSASPNALMQAINVSIGFDRRLAAQ
                     DIAGSQAHAAMLAKTGVISSEDAAQIQSGLSAIVAEMEAGTFPFRDEFEDIHMNVEAR
                     LRELIGPVAGRLHTARSRNDQVALDFRLWVRDACDRSAAQLVALQTALLAKAEAHAAV
                     LMPGFTHLQPAQPVTFGHHLMAYVEMFGRDAARFRDARIRMNECPLGAAALAGSPFPI
                     DRQMTAAALGFDRPTANSLDSVSARDFALEALSAASICATHLSRLAEEIVLWMTPQFG
                     FITLSDAFTTGSSIMPQKKNPDAAELIRAKVGRILGSLSSLTVVMKGLPLAYSKDMQE
                     DKVPTFEAFDALELSLLAMTGMIADLQPNVDRMAAAAGAGFSTATDLADWLVRELDLP
                     FRDAHHITGAAVKRAEQLGVDLADLPLVELQALNQGIGEDVFTVLTPAASSASRVSYG
                     GTAPDQVRSQITRWKDILR"
     gene            2587..2784
                     /locus_tag="PLO65_08755"
     CDS             2587..2784
                     /locus_tag="PLO65_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28376.1"
                     /translation="MKTKAQILSLALAGLALAGCGRLGELERPGPGAEPAKSGTTGTV
                     MDPATDGRTARDAPIPGSNPQ"
     gene            3000..4271
                     /gene="lysA"
                     /locus_tag="PLO65_08760"
     CDS             3000..4271
                     /gene="lysA"
                     /locus_tag="PLO65_08760"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRD28377.1"
                     /translation="MNHFEVRGGELHCEDTPLSRIAEVVGTPVYVYSRATLERHFTVF
                     RQALVEAGLGDPLIAYAVKANSNVAVLRVLGELGAGADTVSEGEIRRALAAGIPGERI
                     VFSGVGKTRGEIAFALTAGVSEINVESEPELTLVAEVAGSLGLRAPVVFRVNPDVAAG
                     GHAKIATGKAENKFGVSFDEAARLYANAANHPHLKPLGVACHIGSQITDLAPFEAAFT
                     RMRRLVEALRGEGLTVERLDLGGGLGVPYFDMPDPPGPEAYAAMVGRVMAGLEDVRFA
                     FEPGRVIAANAGVLLAGVTHLNVRPEGRRFLVLDAAMNDLIRPAMYEAFHDIRPVRAT
                     DATPVPCDVVGPVCETGDTFTRDRLLPPLAAGDLVAFMSAGAYGAAMASEYNSRPLVP
                     EVLVDGDRFAVIRRRPTYDEMLALETPAQWR"
     gene            4277..>5391
                     /locus_tag="PLO65_08765"
     CDS             4277..>5391
                     /locus_tag="PLO65_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HRD28378.1"
                     /translation="MTLRPFAALALAALLAAPALAQTPGVVSPDRLAAHVKALADPAL
                     AGRAPGGPGEPGTIDYLVGQFKALGLEPAGDNGGWTQEVALNRFVVPGDAILSLTVGG
                     GTRPLVQTREIMAATQRPVDRVTIDKAPLVFVGYGVSAPERGWDDFKGVDLKGKIAVF
                     LINDPDFEAGPGDAVAGKFGGQAATYYARWSYKYEEAVRRGAIGALIVHETAGAAYPW
                     TTVVASNGESFDVVRDDPAAARLLLQGWIQRDVAVDLFRKAGLDFEAEKLRARTAAFR
                     PVTLAGASFSADYPLKVDRLVSRNVLARIPGATHPDESIMFSAHWDAFGLGPADATGD
                     TVRHGAADDAIGVAGVIELADGRKLGFQIKQGNMHGNS"
BASE COUNT          771 a         1916 c         1906 g          798 t
ORIGIN      
        1 gacctggccg tggccgggga gagcgcgcgg ttcgccgtct cgggaatcaa tgtcggcctg
       61 ttctgctcga cgccggccgt acccctgtcg cgcgcggtgg gctcgaaggt cgcgctggac
      121 atgctgttca ccggccggtt catcagcgcc gccgaggccc tcgcccacgg cctggtcagc
      181 cgcgtcgcgc cggacgcgga cctcgacgcc gccgtcgacg ccctgaccgg cgagatcgcc
      241 gccaagagcc ctgtcgccgt gcgcaccggc aaggccatgt tcgcccgcca ggccgccctg
      301 ccgctggacc aggcctatgc ctacgccgcc ggggtgatgg ccggaaacat gatggccgag
      361 gacgccggcg agggcatcga cgcgttcatc ggcaagcgca agccggcgtg gagtgggcgg
      421 tagccggggg acatgttcgg attgcgcagc aatccgtacg tgtccccatc catccgcgcg
      481 taaatgatca ccgcccgcgc tcgcttgggg acacgtacgc atttgcgggg caaatgcgaa
      541 catgtccccc gcgtccagct cccccctact ccttcagcag cgtctcgatc agggcccggg
      601 cttccttgcc gttccagtcg gcgccgccgc tcaggcgggc gcgctcgcgg ccctgtttgt
      661 cgtagatgac cgtcgtcggg aagccctgcg ccgccggctt catgccgaag gcgaacttgt
      721 agccgggatc gcggtagagc ttgagcggcg cgttgcgggc gatgtcggcc ctggccagat
      781 tgaggtcggc gtcgcggtcg acgctgatgg ccacgacctg caccggctgg gtctggtagg
      841 cggcctgcag cttcgccagc gtcggcatct ccagccggca tggcgcgcac caggtggccc
      901 acaggttgac caccacgatc tggcccttga agtcggccag ggtcatggcc ttgccgtcgg
      961 cgtcggtgaa gcccgccgtc ggggcgggcg tcggcgcggc gggcaccacc agcttgacca
     1021 gcccgccctt cgcgaattgt cgcaagtccg tgtcgctctt gggtttgatg gaggcgctga
     1081 cgatgacgta tagaaccgcc gcgacgccga tcagggcggc gccccggatg gcccacttca
     1141 acaggtcggg cctgggtttc gccggctgga cttcgctcat atgactgaca aatcctcgaa
     1201 tagggccggt gctggacagg cgatgtgggg cggtcggttc tcggcgagtc ccaacgccct
     1261 gatgcaggcg atcaacgtct ccatcggctt cgaccggcgc ctggcggccc aggatattgc
     1321 cgggtcgcag gcccacgccg ccatgctggc gaagactggc gtcatatcta gcgaggacgc
     1381 ggcgcaaatc cagagcggcc tttcggccat cgtcgccgag atggaagccg ggaccttccc
     1441 gttccgcgac gagttcgagg acatccacat gaatgtggag gcgcggctgc gcgagctgat
     1501 cgggccggtg gccggccggc tgcacaccgc ccgctcgcgc aacgaccagg tggcgctcga
     1561 tttccggctc tgggtgcggg acgcctgcga ccggtcggcg gcccagctgg tcgcgctgca
     1621 gacggcgctg ctggccaagg ccgaagcgca cgccgccgtg ctgatgccgg gcttcaccca
     1681 cctgcagccg gcccagccgg tgacgttcgg ccaccatctg atggcctatg tcgagatgtt
     1741 cggtcgcgac gccgcccggt tcagggacgc gcgcatccgg atgaacgaat gcccgctggg
     1801 cgccgccgcc ctcgccggct cgccgttccc gatcgaccgg cagatgaccg ccgcagcgct
     1861 gggcttcgac cggccgacgg ccaattcgct ggacagcgtg tcagcccgcg acttcgccct
     1921 ggaggccctg tcggcggcca gcatctgcgc cacccacctg tcgcgactgg ccgaagagat
     1981 cgtgctgtgg atgacgccgc agttcggctt catcaccctc agcgacgcct tcaccaccgg
     2041 cagctcgatc atgccgcaga agaagaaccc cgacgcggcc gagctgatcc gggccaaggt
     2101 cgggcggatt ctcggctcgc tgagcagcct gaccgtggtc atgaagggcc tgccgctggc
     2161 ctattcgaag gacatgcagg aggacaaggt cccgaccttc gaggcgttcg acgcgctgga
     2221 gctcagcctg ctggcgatga ccggcatgat cgccgacctg cagccgaacg tggaccggat
     2281 ggccgccgcc gccggggcgg gcttctcgac cgccacggac ctggccgact ggctggtgcg
     2341 cgaactcgac ctgccgttcc gcgacgccca ccacatcacc ggcgcggcgg tgaagcgcgc
     2401 cgagcagctg ggcgtcgacc tggccgacct gccgctggtc gagctgcagg cgctgaacca
     2461 gggcatcggc gaggacgttt tcaccgtgct gacccctgcc gcctcgtcgg caagccgcgt
     2521 aagctacggc ggaaccgcgc cggaccaggt ccggtcccag atcacgcgct ggaaggacat
     2581 cctgcgatga agaccaaggc ccagatcctg tctctggccc tcgcgggcct ggcgctggcc
     2641 ggctgcggcc ggctgggcga gctcgaacga ccgggcccgg gcgccgagcc ggcgaaatcc
     2701 ggcacgaccg gcacggtgat ggatccggcg accgacggcc ggacggcgcg cgacgcgccg
     2761 atccccggct ccaatccgca gtagaaagcc actctgttgt cggctgacgc ttcacgcatg
     2821 gatcccgcac ccactcaact gtcatcccgg acgtccgaag gacgatccgg gacccagatt
     2881 gaatcccaac cgatgtgtgt gaggccgaat ctgggtcccg gctccgcgcc tcgctccggc
     2941 cgggatgaca gtgccggcgt tgatggccgg accgacttcg aacccggatt ggaccaggcg
     3001 tgaaccattt cgaggtccgg ggcggcgagc tccattgcga ggacacgccc ctctcccgga
     3061 tcgcggaggt cgtggggacg cccgtctatg tctattcgcg cgcgaccctg gagcggcact
     3121 tcaccgtgtt ccgtcaggcc ctcgtcgagg ccggcctcgg cgacccgctg atcgcctatg
     3181 cggtcaaggc caacagcaac gtggccgtgc tgcgggtgct gggcgagctg ggcgcgggcg
     3241 ccgacaccgt gtccgagggc gagatccgcc gggcgctggc cgccggcatc ccgggcgagc
     3301 ggatcgtctt ctccggcgtc ggcaagaccc gcggcgagat cgccttcgcc ctgacggccg
     3361 gggtgtcgga gatcaacgtc gagtccgagc cggagctgac gctggtcgcc gaggtggccg
     3421 ggagcctcgg cctgcgggcg ccggtcgtgt tccgggtcaa tcccgacgtc gccgccggcg
     3481 gccacgccaa gatcgccacc ggcaaggccg agaacaagtt cggcgtgtcg ttcgacgagg
     3541 ccgcgcgact ctacgccaac gccgccaacc atccgcacct caagccgctc ggcgtggcct
     3601 gccacatcgg cagccagatc accgacctgg ccccgttcga ggcggccttc acgcgcatgc
     3661 gccgcctggt cgaggccctg cgcggcgagg gcctgaccgt cgagcggctc gacctcgggg
     3721 gcgggctggg cgtgccctat ttcgacatgc ccgacccgcc agggccggag gcctatgcgg
     3781 cgatggtggg gcgggtcatg gccgggctgg aggacgtgcg cttcgccttc gagccgggcc
     3841 gggtgatcgc ggccaacgcc ggggtgctgc tggccggggt gacccatctg aacgtgcggc
     3901 cggaaggccg gcggttcctg gtgctcgacg cggcgatgaa cgacctgatc cggcccgcga
     3961 tgtacgaggc gttccacgac atccggccgg tccgggccac cgacgcgacg ccggtcccct
     4021 gcgacgtggt cgggccggtc tgcgagacgg gggacacctt cacgcgcgac cggcttctgc
     4081 cgccgctggc ggccggcgac ctggtcgcct tcatgagcgc cggggcctac ggcgcggcca
     4141 tggccagcga gtacaattcc cgccccctgg ttcccgaggt gctggtcgat ggcgaccggt
     4201 tcgcggtgat ccggcggcgg ccgacctatg atgaaatgct ggcgctcgaa acgccggcgc
     4261 agtggcggtg acccctatga cccttcgacc tttcgcggcc ctggccctgg ccgctctgct
     4321 ggcggcgccg gccctggccc agacgccggg cgtggtctcg cccgaccggc tggccgccca
     4381 cgtcaaggcc ctggccgacc cggccctggc cggccgcgcg cccggtggtc cgggcgagcc
     4441 ggggacgatc gactacctgg tcggccagtt caaggcgctg gggctcgaac ccgccggcga
     4501 caacggcggc tggacccagg aggtggccct caaccgcttc gtcgtgcccg gcgacgcgat
     4561 cctcagcctg accgtcggtg gcgggacccg gccgctggtc cagacccgcg agatcatggc
     4621 cgccacccag cggccggtgg accgggtgac catcgacaag gccccgctgg tgttcgtcgg
     4681 ctacggcgtg agcgcgcccg agcgcggctg ggacgacttc aagggcgtcg acctgaaggg
     4741 caagatcgcc gtcttcctga tcaacgaccc cgacttcgag gccgggcccg gcgacgcggt
     4801 cgcgggcaag ttcggcggcc aggcggcgac ctactacgcc cgctggagct acaagtacga
     4861 ggaagcggtt cggcgggggg ccatcggcgc cctgatcgtg cacgagacgg ccggagcggc
     4921 ctatccctgg accacggtgg tggcctccaa cggcgagagc ttcgacgtcg tgcgcgatga
     4981 cccggccgcc gcgcgcctgc tgctgcaggg ctggatccag cgcgacgtgg ccgtcgacct
     5041 gttccgcaag gccgggctcg acttcgaggc cgagaagctg cgcgcccgca cggccgcctt
     5101 ccggcccgtg accctggccg gggcgagctt ctcggccgac tatccgctca aggtcgaccg
     5161 gctcgtcagc cgcaatgtgc tggccaggat tcccggcgcg acccaccccg acgagagcat
     5221 catgttcagc gcccactggg acgccttcgg cctcggcccg gccgacgcga ccggcgacac
     5281 cgtgcggcac ggcgcggccg acgacgcgat cggcgtggcc ggcgtcatcg agctggccga
     5341 tgggcgcaag ctgggctttc agatcaagca ggggaacatg catgggaact c
//
LOCUS       DAORNX010000173         5385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11287_length_5385_cov_6.760225, whole genome shotgun sequence.
ACCESSION   DAORNX010000173 DAORNX010000000
VERSION     DAORNX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_5385_cov_6.760225"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLO65_08770"
     CDS             complement(<1..400)
                     /locus_tag="PLO65_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRD28379.1"
                     /translation="MALDARDATDRVDQLIVLTDRLTALMAQQCLAFEQRRPQDAAAQ
                     MEELSKLANIYRHESTRVRQAPTLIEAAPLEQRMRLMQATEAFDAVLARQGRALEAAR
                     TVTEGLVRAIAEEVASQRTTGAGYGPAATGK"
     gene            complement(415..732)
                     /locus_tag="PLO65_08775"
     CDS             complement(415..732)
                     /locus_tag="PLO65_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRD28380.1"
                     /translation="MAAPVALPADILLGQAQAATASTADLARKRKSEEAARSFEASFL
                     STMLQPMFEGLSTEAPFGGGQGEAMFKSFMTDAIAKQTAKRGGLGIGDMVQREMLKLQ
                     GLS"
     gene            complement(732..1838)
                     /locus_tag="PLO65_08780"
     CDS             complement(732..1838)
                     /locus_tag="PLO65_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRD28381.1"
                     /translation="MRRSIVGSLLLAAALMVAGPSFASSRIKDVVAFEGVRENQLTGY
                     GLVVGLNGTGDNLRNAPMTRQSLEAMFERLGVNVRDTNLNTKNVAVVMVTARLPPFAA
                     AGSAIDVTVSAAGDAKSLQGGTLLVTALMGADGQVYAVAQGTVQTGSVSASGASGSSV
                     SKGVPTAGRIAGGATIERETGFQMGQMSEMRMTLRNPDFTTAQRIATVINGRFPGTAV
                     ADNPTIVTLRPPPGQSMVGFMTAVETLEIEPDAPAKVIVDEVSGVIVMGEAVRISTVA
                     IAQGNLTISVQETPQVSQPQPFSRGGETVVTSQSNVAVDEERGRQLITLRNGASLSSL
                     VAGLNALGVTPRDMISILQAIKAAGALQADIEVM"
     gene            2040..2477
                     /locus_tag="PLO65_08785"
     CDS             2040..2477
                     /locus_tag="PLO65_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HRD28382.1"
                     /translation="MKVSGSSGVESLGSARAAGRPAQGQGGFSLPVIGGAGAAPDVGR
                     SAGVGGVSSVDALIALQDVGGPLERRRRAVGRAGRILDVLDEVKVALIGGEVSGRDLD
                     RLMRAVREERMTTDDGPLEGLLNEIETRAAVELAKLEQARVAA"
     gene            2574..3002
                     /gene="dksA"
                     /locus_tag="PLO65_08790"
     CDS             2574..3002
                     /gene="dksA"
                     /locus_tag="PLO65_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRD28383.1"
                     /translation="MATATVLAEQVDYRPSEDEPFMNERQLEYFKQKLLNWKDEILRE
                     SRETVTHLQKETENHPDLADRATSETDRALELRTRDRQRKLISKIDEALRRIEDGSYG
                     YCEETGEPITLARLEARPVATLSLEAQERHERRERVHRDD"
     gene            3160..4500
                     /gene="gltX"
                     /locus_tag="PLO65_08795"
     CDS             3160..4500
                     /gene="gltX"
                     /locus_tag="PLO65_08795"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRD28384.1"
                     /translation="MTVRVRFAPSPTGRLHVGNIRTGLINWLFARSQGGQFVLRIDDT
                     DIERSTLEYEQGIETDLTWLGMVWDEKHKQSARFPLYREAMERLKASGRLYPAYETGE
                     ELDRRRKVQLSRGQPPIYDRAALKLTAAERATLEADGRRPHWRFRLEGKRVRWHDLAR
                     GDCEVDTASMSDPVLIREDGAFLYTLPSVVDDIDMAITHVIRGEDHVTNTGAQIEIFE
                     ALGAKAPHFAHTTLLVGADGEGLSKRLGSMSIMDLRESGYEPLAVVSHLARIGTSDPL
                     EPGESLDALAAAFTFGKMARHPGRYDPADLSRLNAAVLHAMPYAEAQPRLANLGADLG
                     EAFWLAIRSNLGLFSGVSNWVRVVSGAVDPLIAPEDAAVVAAAGELLPAALDGGTWSA
                     WTTAVKERTGAKGRGLFMPLRRALTGLDHGPEMGPLLPLIGRDKAARRLAGETA"
     gene            complement(4526..5131)
                     /locus_tag="PLO65_08800"
     CDS             complement(4526..5131)
                     /locus_tag="PLO65_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRD28385.1"
                     /translation="MSSLSAPVDSVCVYCGSSNDADPAFLAAAYAIGGDIARAGLKFV
                     YGGGGVGLMGASARGAHDAGGRVLGIIPDFLQDREQPFSDVETIVVHSMHERKMMMFE
                     RADSFAVLPGGIGTLEEIVELLSWQRLGLHAKPVVFYNPRGFWDPLFELFHHTIRERL
                     SPPEFARAWRSVDVVEDLVPAILAMGEDSGGGADPRTSDLT"
BASE COUNT          830 a         1790 c         1921 g          844 t
ORIGIN      
        1 ccttgccggt ggcggccggc ccgtagccgg ccccggtggt gcgctgagag gcgacctcct
       61 cggcgatggc ccggaccagg ccctcggtga cggtgcgggc cgcctccagg gcgcgcccct
      121 ggcgggccag cacggcgtcg aacgcctcgg tcgcctgcat cagccgcatg cgctgctcga
      181 gcggcgccgc ctcgatcagg gtcggcgcct ggcggacgcg ggtcgactca tgccggtaga
      241 tgttggccag cttcgacagc tcctccatct gcgccgcggc gtcctgcggc cggcgctgct
      301 cgaaggccag acactgctgg gccatcaggg cggtcaggcg gtcggtgagg acgatcagct
      361 ggtcaacgcg atcggtcgcg tcgcgggcgt cgagggccat gggaggacgt ctttctaact
      421 gagaccttgg agcttgagca tttcgcgctg gaccatgtcg ccgatcccca ggccgccgcg
      481 cttggcggtc tgtttggcga tggcgtccgt catgaaggac ttgaacatcg cctcgccctg
      541 gccgccaccg aacggcgcct cggtcgacag cccttcgaac atcggctgca gcatggtcga
      601 caggaacgag gcctcgaatg agcgggcggc ctcctcgctc ttgcgcttgc gcgccaggtc
      661 ggcggtcgag gccgtcgcgg cctgggcctg gcccaggagg atgtcggcgg gaagggcgac
      721 gggagcggcc atcacatcac ctcgatgtcg gcttgcaggg cgccggcggc cttgatcgcc
      781 tgcaggatgg agatcatgtc gcgcggcgta acgcccaggg cgttcaggcc ggccacgagg
      841 ctcgacaggg acgcgccgtt gcgcagggtg atcagctggc ggccccgttc ctcgtcgacg
      901 gcgacattgg actggctggt gacgaccgtc tcgccgcccc ggctgaacgg ctggggctgg
      961 ctgacctggg gcgtttcctg cacggagatg gtcaggttgc cctgggcgat ggccacggtg
     1021 gagatgcgca ccgcctcgcc catcacgatc acgccgctga cctcgtcgac gatcaccttg
     1081 gcgggcgcgt cgggttcgat ttcgagggtt tccacggccg tcatgaagcc gaccatgctt
     1141 tgtccgggcg gcgggcgcag ggtgacgatg gtcgggttgt cggccaccgc cgtgccggga
     1201 aaccggccgt tgatcaccgt ggcgatccgc tgggcggtgg tgaagtccgg attgcgcagg
     1261 gtcatgcgca tctcggacat ctggcccatc tggaagcccg tctcccgctc gatggtcgcg
     1321 ccgccggcga tccggccggc cgtgggcacg cccttgctga ccgaagagcc tgacgcgccc
     1381 gaggccgaga ccgagccggt ctgaacggtg ccctgggcca ccgcatagac ctggccgtcg
     1441 gcgcccatca gggcggtgac cagcagtgtg ccgccctgca ggctcttggc gtcgccggcg
     1501 gccgagacgg tgacgtcgat ggccgagccg gccgcggcga acggcggcag cctggccgtg
     1561 accatgacca ccgcgacgtt ctttgtgttg agattggtgt cgcggacgtt gaccccgagc
     1621 cgttcgaaca tcgcctcgag gctctggcgg gtcattggcg cgttgcgcag gttgtcgccg
     1681 gtgccgttaa gtccaaccac caggccgtag ccggtcagct ggttctcgcg cacgccctcg
     1741 aaggcgacga cgtccttgat ccgcgaactc gcgaaactcg ggccggcgac catcaacgcc
     1801 gccgccagca acagactgcc tactatcgat cgacgcatct cgttcccgcc gcacgctgca
     1861 ctgcccgcgc atccatgcga gatccgcgcc agataaaact gccgcaaaag caataggtta
     1921 tcggcccctc ccccgtcctg gcagggtgtc gcggcagaat ttgcccggca ttaaccatgc
     1981 tgcaagcctg agcgcggaca gtcggcgctc aggatcggtt ggagcggcgt agcgcgttca
     2041 tgaaagtctc gggatccagc ggtgtcgaat cgctcggaag cgccagggcg gcgggtcgtc
     2101 cggcgcaggg acagggcggc ttctcgctgc cggtcatcgg tggcgccggc gctgcgccgg
     2161 acgtggggcg cagcgccgga gtcggaggcg tcagctcggt cgacgccctg atcgctctcc
     2221 aggatgtcgg ggggccgctc gagcgccgtc ggcgcgcggt cggccgggcc ggccgcattc
     2281 tcgatgtgct cgacgaggtg aaggtcgccc tgatcggcgg agaggtgtct ggccgcgacc
     2341 tggaccgtct gatgcgggcg gtgcgcgagg agcgcatgac gaccgacgac gggccgctgg
     2401 agggcctgct caacgagatc gaaacccgcg cggcggtcga actggcgaag ctcgaacagg
     2461 cgagggtcgc ggcatgaacg cccgcccaac gcgagcttca ttgatcgttg cagagctttt
     2521 gttataaggc cccgctcttg tgagcgtggc gtaaaggggg cgtgaggcgt caaatggcga
     2581 cggctacggt tctggctgaa caggtcgact atcgtccttc cgaggacgag cctttcatga
     2641 acgagcgtca gctcgagtat ttcaagcaaa agctgctgaa ctggaaggat gagatccttc
     2701 gcgaatctcg cgagacggtg acacatctcc agaaggagac cgagaatcat ccggacctgg
     2761 ccgatcgcgc cacgtcggag accgaccggg ctctggaact tcgcacccgt gaccggcagc
     2821 ggaagcttat ctcgaagatc gatgaggccc tgcggcgaat cgaggacggg tcctacggct
     2881 actgcgagga gacgggcgag cccatcaccc tcgcccggct ggaagcccgc ccggtggcga
     2941 ctctgagcct cgaggcccag gagcggcacg agcgccgcga acgggtccat cgcgacgact
     3001 gatctgcagg ggacctgtac cgaaggtgcg agtccccgat tttcgaagcg gcgaattttg
     3061 gggacacgta ccttcggtac atgtcccctc cgtcctctca tcttgacttc gagcctccca
     3121 gagccgacct agccccgctc tgagaaggac gtccatccca tgaccgtaag ggtccgtttc
     3181 gcgccgtcgc ccaccgggcg gctgcatgtc ggcaacattc gcaccggcct gatcaactgg
     3241 ctgttcgcgc ggtcgcaggg cgggcagttc gtgctgcgca tcgacgatac ggacatcgag
     3301 cgctcgaccc tcgaatacga gcagggcatc gagaccgacc tgacctggct tggcatggtc
     3361 tgggacgaga agcacaagca gtcggcgcgc ttcccgctct atcgcgaggc gatggagcgg
     3421 ctgaaagcca gcggccgcct gtatccggcc tacgagaccg gcgaggagct cgaccgccgc
     3481 cgcaaggtgc agctgtcgcg cggccagccg ccgatctacg accgcgccgc gctcaaactg
     3541 accgccgccg agcgggcgac gctggaggcc gatggccgcc ggccgcactg gcggttccgg
     3601 ctcgagggca agcgggtcag gtggcacgat ctggcccgcg gcgactgcga ggtcgatacc
     3661 gcctcgatgt cggaccccgt gctgatccgc gaggacggcg ccttcctcta caccctgccg
     3721 tcggtggtcg atgatatcga catggccatc acccacgtga tccgcggcga ggatcacgtg
     3781 accaatacgg gcgcgcagat cgagatcttc gaggccctgg gcgcgaaggc gccgcacttc
     3841 gcccacacca ccctgctcgt cggggcggac ggcgagggcc tgtccaagcg gctgggctcg
     3901 atgtcgatca tggacctgcg cgagagcggc tacgagcccc tggccgtggt ttcgcatctg
     3961 gcgcggatcg gcacctccga cccccttgaa cccggcgaga gtctggatgc cctggccgcg
     4021 gccttcacct tcggcaagat ggcgcgtcat ccgggccgct acgatccggc cgacctgtca
     4081 cggctgaacg cggcggtgct gcacgccatg ccctacgccg aggcgcagcc gcggctggcc
     4141 aacctgggcg cggacctggg cgaggccttc tggctggcga tccgttcgaa cctgggcctg
     4201 ttctccgggg tctcgaactg ggtccgggtg gtcagcggcg cggtcgaccc gctgatcgcg
     4261 cccgaggacg ccgcggtcgt cgcggcggcc ggtgaactgc tgcccgcagc cctcgacggc
     4321 gggacctgga gcgcctggac cacggcggtg aaggagcgga ccggggccaa ggggcgtggt
     4381 ctgttcatgc cgctgcgccg ggccctgacg ggcctggacc atgggcccga gatggggcca
     4441 ttgctgccgc tgatcgggcg ggacaaggcc gcgcggcgtc tggccggcga aaccgcctga
     4501 ggtcgggggc cgcggccccc gccggtcagg tcagatcgct cgtgcgcggg tccgccccgc
     4561 cgccggagtc ctcgcccatg gcgaggatgg cgggcaccag gtcctcgacg acgtcaacgg
     4621 accgccacgc ccgcgcgaac tccggcggcg acagccgttc gcgaatagtg tggtggaaca
     4681 gctcgaacag cggatcccag aagccgcggg gattgtagaa cacgaccggc ttggcgtgca
     4741 ggcccagccg ctgccaggac agcagttcga cgatctcctc cagcgtccct atgccgcccg
     4801 gcaacacggc gaacgagtcg gcgcgctcga acatcatcat cttccgctcg tgcatggagt
     4861 ggacgacgat cgtttcgacg tcactgaagg gctgttcgcg atcctgcagg aagtcgggaa
     4921 tgatgcccag gacgcgaccg cccgcgtcgt gggccccgcg cgcgctggcg cccatcaacc
     4981 cgaccccgcc gccgccatag acgaacttca gccccgcgcg ggcgatatcg ccgccaatgg
     5041 cgtaggcggc ggcgaggaag gcggggtcgg cgtcgttgga ggaaccgcag tagacgcaga
     5101 cggaatccac gggggcggac aggctggaca tcgccgcggg gctccaggga tgaaaaaggc
     5161 gcgctcggtg gcgcgccgtg tgactatgat ccgcatgatc gggtgttgag cgcggggttg
     5221 caagagtcgt gtcggatcgg ttcagcgtca tggcgggtct cgtcccgctt ctgttcctcg
     5281 ccggctgcgg cgacggggcg cagccggtct ggaagggcga ggcgtcgaag accgcggcgg
     5341 ttgccggcta cctggcgccg ccgctcgtgc tggcgacgcg aaccg
//
LOCUS       DAORNX010000174         5358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11366_length_5358_cov_8.209504, whole genome shotgun sequence.
ACCESSION   DAORNX010000174 DAORNX010000000
VERSION     DAORNX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11366_length_5358_cov_8.209504"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLO65_08805"
     CDS             complement(<1..275)
                     /locus_tag="PLO65_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRD28386.1"
                     /translation="MAGLTAARTVVVLGSTGSIGVSTLDLFDKSGCEVDILALTAGRN
                     VERLAQQALRWRPRIAVIEQDSLLGELRERLSGSGIATAGGAAAVEEA"
     gene            complement(263..1090)
                     /locus_tag="PLO65_08810"
     CDS             complement(263..1090)
                     /locus_tag="PLO65_08810"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRD28387.1"
                     /translation="MTSSPPARRFDWNNLFVRVASASVLIPAVIVALWFGGWAWLLLV
                     SVGVALLANEWGVMSAPVAPARIATAVTVGVLAAVFAMYFHQPTLAWGLVVVGAALAA
                     LVARGTAERPADAAYGVIYIAPCAIALLWLRGEEHGRSWATMLFAVTWSADICAFLVG
                     SALKGPKLWPRFSPNKTWSGFFGGLVGAMIAAVLVATFSDATLVWYAAGLVGLAGGLA
                     TMAGDLWESALKRRFGVKDSGDLIPGHGGLLDRVDGLMFASVVIAGARLIDHWGWPA"
     gene            complement(1068..1802)
                     /gene="uppS"
                     /locus_tag="PLO65_08815"
     CDS             complement(1068..1802)
                     /gene="uppS"
                     /locus_tag="PLO65_08815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRD28388.1"
                     /translation="MTPTDGPLHVAIIMDGNGRWAKQRGLPRTFGHREGVKALKRTVE
                     AASGLGVGTLTVFGFSTENWRRPMAEVSELMSLLKAYVESDLDRLVREGVKVRVIGRR
                     TGLKPDVLAIIDRAEARTADNTSFLLQVAFNYGGRADIADAARRFAEAVEQGRARAAD
                     LDETLFEQFLSTADTPAPDLIVRTSGERRISNFLLWECAYAELVFQDVYWPDYGPDHL
                     KAAIAEFRARERRYGGAASDDVLAAG"
     gene            complement(1851..2417)
                     /gene="frr"
                     /locus_tag="PLO65_08820"
     CDS             complement(1851..2417)
                     /gene="frr"
                     /locus_tag="PLO65_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRD28389.1"
                     /translation="MAAGEKPVLSNYKSRMEKAAQALKEEFASLRTGRASASLLDQVM
                     VEAYGNTVPLNQVGSVSVPEPRSISVSVWDRGLVVSVEKAIRNSGLGFNPVVEGQNLR
                     VPIPPLTEERRRDLVKIAGKYAEQQKIAVRNVRRDAMDDLKKAEKDGTITQDEHRRMD
                     GEVQKMTDEAVKRIDETLKTKEQEIMQV"
     gene            complement(2438..3160)
                     /gene="pyrH"
                     /locus_tag="PLO65_08825"
     CDS             complement(2438..3160)
                     /gene="pyrH"
                     /locus_tag="PLO65_08825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRD28390.1"
                     /translation="MPTKTYKRILVKVSGEVLMGDSAFGIDMGTVEAVARDLKQVADS
                     GVELCLVIGGGNIFRGLAGAAKGMERASADYMGMLATVMNALAMQDALERIGVSTRVQ
                     SAIPMPTVCEPLIRRRAERHLEKGRVVIFAAGTGNPFFTTDSGAALRAAEMNCDALFK
                     GTSVDGVYTADPKKDKSAQRFDRLTYHDVLSRDLKVMDASAISLMRENGIPIVVFSIR
                     ERGNLMKVLRGEGVHTVISEAA"
     gene            complement(3261..4199)
                     /gene="tsf"
                     /locus_tag="PLO65_08830"
     CDS             complement(3261..4199)
                     /gene="tsf"
                     /locus_tag="PLO65_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRD28391.1"
                     /translation="MAEITAALVKDLREKTGVGMMDCKKALQENNGDMDASIDWLRAK
                     GLSKAAKKADRAAAEGLIAIATRKDGAGMSAAVIELNAETDFVARNELFQTAARKTAL
                     AALDVAGGVEDIVAATTADGEVIGDMVTQLIATIGENMMVRRSASFTVSPGAIGAYVH
                     NAVAPDLGKIGVLVALEGAGDQAALQELGRKIAMHVAATNPMSLSTDDLDQANVERER
                     AVFTEQALASGKPESVVEKMVEGRIRKFYEEVVLLKQAFVMNPDQTIEQLVEATGKEL
                     GAPIKVVGFVRLALGEGVEKEVSDFAAEVASMTGGN"
     gene            complement(4299..5189)
                     /gene="rpsB"
                     /locus_tag="PLO65_08835"
     CDS             complement(4299..5189)
                     /gene="rpsB"
                     /locus_tag="PLO65_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRD28392.1"
                     /translation="MALPEFSMRQLLEAGAHFGHQTHRWNPKMDRYIFGSRSNIHIID
                     LSQSIPLLHQALVKVREVAAAGGRVLFVGTKRQASDPVATAAKRCAQYYVNHRWLGGT
                     LTNWRTISGSISRLRELEGVLEGDGGGRSKKELLNLTRERDKLELSLGGIKNMGGIPD
                     IMFVIDTNKEAIAILEARKLNIPVIAILDTNCDPDGITYPIPGNDDAARAIQLYCDLI
                     ADSVLDGMAAGVVASGVDIGASEAPVEPALAREMTPEPAPAAAAAEPEAVAAPEPAPA
                     EAQAATEAALDAVPEEPAAS"
BASE COUNT          812 a         1833 c         1814 g          899 t
ORIGIN      
        1 gcttcctcga ccgccgcggc cccgccggcg gtggcgatgc cgctgccgct cagccgttcg
       61 cgcagctctc cgagcagcga gtcctgctcg atcaccgcga ttcgcggacg ccagcgcagc
      121 gcctgctggg ccagccgctc gacattgcgc ccggccgtca gggccaggat gtcgacctcg
      181 cagcccgact tgtcgaacag gtcgagggtc gaaaccccga tcgatccggt cgagccgaga
      241 acgacaacgg tgcgggcggc ggtcaagccg gccagcccca atgatcgatc aaccgagccc
      301 cggcgatgac caccgaagcg aacatcaggc cgtcgacacg gtccagcagg cccccatgtc
      361 ccggaatcag gtcgcctgaa tccttcacgc caaaccgccg tttcaaggcc gactcccaaa
      421 gatcgccagc catcgtggcc aggccgccgg ccaggccgac gaggccggcc gcataccaga
      481 ccagggtcgc atcggaaaac gtcgcgacca ggaccgcggc gatcatcgca ccgacgagcc
      541 cgccaaagaa gcccgaccag gtcttgttcg gcgaaaagcg cggccagagc ttgggcccct
      601 tgagcgcact gccgaccaga aaggcgcaga tgtcggccga ccaggtcacc gcgaacagca
      661 tggtcgccca gctgcgaccg tgctcctcgc cccgcagcca cagcagggcg atggcgcagg
      721 gcgcgatgta gatgacgcca taggcggcgt cggcgggccg ctcggccgtg ccccgggcga
      781 cgagagccgc cagggccgcc ccgaccacga ccagccccca cgccagggtg ggctggtgga
      841 agtacatggc gaacaccgcc gccaggacgc cgaccgtcac ggccgtggcg atcctcgccg
      901 gcgccaccgg ggcgctcatc acgccccatt cgttggccag cagggccacg ccgacggaga
      961 ccagcagcag ccaggcccag ccgccgaacc acagggcgac gatgaccgcc ggaatcagca
     1021 cgctggccga ggcgacgcgg acgaacaggt tgttccagtc gaatcgccta gccggcggcg
     1081 aggacgtcat cggacgcggc tccgccataa cgccgctcac gggcccggaa ttcggcgatc
     1141 gccgccttga ggtggtcggg accgtagtcc ggccagtaga cgtcctggaa caccagttcg
     1201 gcgtaggcgc attcccagag caggaagttg gagatccgcc gttcgccgct ggtgcggacg
     1261 atcaggtccg gcgcgggcgt gtcggcggtc gacaggaatt gctcgaacag cgtctcgtcg
     1321 agatcggcgg cccgcgccct gccctgctcg accgcctcgg cgaaccggcg tgccgcgtcg
     1381 gcgatatccg cccgcccacc gtagttgaag gcgacctgca acagaaaact ggtgttgtcg
     1441 gccgtgcggg cctcggcccg gtcaatgatg gccaggacat ccggtttgag gcccgtgcgg
     1501 cggccgatca cccggacctt gacgccttcc cgaaccagac ggtcgaggtc gctctccaca
     1561 taggccttga gcaggctcat cagttcggag acctcggcca tcggccggcg ccagttctcc
     1621 gtggagaagc cgaacacggt cagggtgccg acgccgaggc cggacgcggc ctcaaccgtg
     1681 cgcttgaggg ccttgacgcc ctcccggtga ccgaaagtgc gtggcaggcc gcgttgtttg
     1741 gcccagcgcc cgttgccatc catgatgatg gcgacatgca gcggcccgtc cgtcggcgtc
     1801 atcgccactc cctcatgtcc gtcgcgcgcc atccccgcga gacgtacagc ctaaacctgc
     1861 atgatctctt gttctttggt tttcaaggtc tcgtcgatgc gcttgacggc ctcgtcggtc
     1921 atcttctgaa cctcaccgtc catccggcgg tgctcgtcct gggtgatggt cccgtccttc
     1981 tcggccttct tcaggtcgtc catcgcgtcg cgacgcacgt tgcggacagc gatcttctgc
     2041 tgttccgcgt atttgccggc gatcttgacc aggtcccggc ggcgctcctc ggtcagcggc
     2101 gggatcggca cccgcaggtt ctgcccctcg acgaccggat tgaagcccag gccggagttg
     2161 cggatggcct tctcgaccga gaccaccagg ccgcgatccc agacgctgac gctgatcgaa
     2221 cgcggctcgg gcacgctaac cgagccgacc tggttcagcg gcacggtgtt gccgtaggcc
     2281 tcgaccatca cctggtcgag caggctggcg gaggcgcggc cggtgcgcag gctggcgaac
     2341 tcctccttca gagcctgggc ggccttctcc atccggctct tgtagttgct cagcacgggt
     2401 ttctcgccgg cggccatgtc caggctccct cttcttccta tgcagcttcg gagatcacgg
     2461 tgtgaacgcc ctcgccgcgc aacaccttca tcagattgcc ccgctcgcgg atcgagaaga
     2521 ccacgatcgg aatgccgttc tcgcgcatca ggctgatcgc cgaggcgtcc atgaccttca
     2581 ggtcgcgcga caggacatcg tgataggtca gccggtcaaa ccgctgggcc gacttgtcct
     2641 tcttcggatc ggccgtgtag acgccgtcca cgctggtgcc cttgaacagg gcgtcgcagt
     2701 tcatttccgc cgcccgcagc gccgcgccgc tgtcggtggt gaagaacgga ttgccggtgc
     2761 cggcggcgaa gatcaccacc cggccctttt cgagatggcg ttcggcgcgg cggcggatca
     2821 gcggttcgca gacggtcggc atgggaatgg ccgactgcac gcgggtcgag acgccgatgc
     2881 gctcgagcgc gtcctgcatg gccagggcgt tcatcaccgt ggcgagcatg cccatgtagt
     2941 cggcgctggc ccgctccatg cccttggccg cgccggccag gccccggaag atgttgccgc
     3001 cgccgatgac gaggcacagc tcgacgccgc tgtcggcgac ctgcttgagg tcacgggcca
     3061 cggcctcgac ggtgcccata tcgatgccga aggcggagtc gcccatcagc acctcgccgg
     3121 acaccttcac caggattcgc ttgtaggtct tggtcggcat gggtttccgg gcggcatgcg
     3181 agtcagttgg cggtcaacat aaaccaaggg cgcccccagc gttagccgga ggcgccctct
     3241 tcagaacgtc agcagcggga tcagttcccg ccggtcatcg aggcgacttc ggcggcgaag
     3301 tcgctgactt ccttctcgac gccctcgccc agggccaggc gcacgaagcc gaccaccttg
     3361 atcggcgcgc ccagctcctt gccggtggcc tcgaccagct gttcgatggt ctggtccggg
     3421 ttcatgacga aggcctgctt gagcagcacg acttcctcgt agaacttgcg gatccggccc
     3481 tcgaccatct tctcgaccac cgactcgggc ttgcccgagg ccagcgcctg ctcggtgaac
     3541 acggcgcgtt cgcgttcgac gttggcctgg tccaggtcgt cggtcgacag cgacatcgga
     3601 ttggtcgcgg ccacgtgcat ggcgatcttg cggccgagct cctgcagggc cgcctggtcg
     3661 ccggcgcctt ccagcgcgac gagcacgccg atcttgccga ggtccggcgc gacggcgttg
     3721 tgcacatagg cgccgatggc gcccggcgag acggtgaagc tggccgagcg gcgcaccatc
     3781 atgttctcgc cgatcgtggc gatcagctgg gtgaccatgt cgccgatgac ctcgccgtcg
     3841 gccgtggtgg ccgcgacgat gtcctcgacg ccgccggcga cgtcgagcgc cgccagggcg
     3901 gtcttgcgcg cggccgtctg gaacagctcg ttgcgggcga cgaagtcggt ctcggcgttc
     3961 agctcgatga ccgcggcgct catgcccgcg ccgtccttgc gggtggcgat ggcgatcagg
     4021 ccctcggccg ccgcgcggtc ggccttcttg gccgccttgc tgaggccctt ggcgcgcagc
     4081 cagtcgatcg aggcgtccat gtcgccattg ttttcctgca aggccttctt gcagtccatc
     4141 atgccgacgc cggtcttttc gcgcaggtcc ttgaccagcg ccgcggtgat ttccgccatg
     4201 tcatgtcctc ctgagttcgg acttataggg gtgcggccgg gcgttaccgc ccggctgcga
     4261 agggtgtgtg aagctcaagg tccccgtgcg ggcgacgatc agctggcggc gggctcttcc
     4321 ggcacggcgt cgagcgccgc ttcggtggcc gcctgggctt cggccggagc cggttccggg
     4381 gccgcgacgg cctccggttc agcggcggcg gcggcgggag cgggctccgg cgtcatctct
     4441 cgggcgagcg cgggctcgac cggggcttcc gaggcgccga tgtcgacgcc gctggccacc
     4501 acgccggcgg ccatgccgtc gagcacggag tcggcgatca ggtcgcagta cagctggatg
     4561 gcgcgggccg cgtcatcgtt gcccgggatc ggataggtga tgccgtcagg atcgcagttg
     4621 gtgtccagga tcgcgatgac cgggatgttc agcttgcggg cctcgaggat ggcgatcgct
     4681 tccttgttgg tgtcgatcac gaacatgatg tcggggatgc cgcccatgtt cttgatgccg
     4741 cccaggctca gctcgagctt gtcgcgctcg cgggtcaggt tcagcagttc cttcttggaa
     4801 cggccgccgc cgtcgccctc gagcacgcct tccagctcgc gcaggcgcga gatcgagccg
     4861 gagatggtgc gccagttggt cagggtgccg ccgagccagc ggtgattgac atagtactgg
     4921 gcgcagcgct tggcggccgt ggccaccggg tccgacgcct ggcgcttggt gccgacgaac
     4981 agcacccggc cgcccgcggc cgcgacttcg cggaccttga cgagggcctg gtgcagcagc
     5041 gggatcgact gcgacagatc gatgatgtgg atgttcgagc gcgagccgaa gatgtagcgg
     5101 tccatcttcg ggttccagcg gtgcgtctgg tggccgaagt gggcgcccgc ttcaagcagc
     5161 tgacgcatgg agaactcggg gagagccatg gggtcttgtc ctttttccgg ttatcgcctc
     5221 cgcggacaaa cccctgatct ctcagggacc ggaacggatt ggacgggatg tctccccggc
     5281 cgacccgaac gtccgcgtgc gtgatgaggc gcgcatgtag gggatggagg gcggaaatgc
     5341 aagcccgggt ggggctgg
//
LOCUS       DAORNX010000175         5330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11449_length_5330_cov_6.194502, whole genome shotgun sequence.
ACCESSION   DAORNX010000175 DAORNX010000000
VERSION     DAORNX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11449_length_5330_cov_6.194502"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>334)
                     /locus_tag="PLO65_08840"
     CDS             complement(<1..>334)
                     /locus_tag="PLO65_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRD28393.1"
                     /translation="GQIAGPRVVEHMVDAVLSFEGERGYPFRILRGAKNRFGATDEIG
                     VFEMGDAGLTEVINPSALFLNAGGERAAGAAVFAGIEGSRPVLVEFQALVAPSAYGTP
                     RRAVVGWDS"
     assembly_gap    335..409
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<410..988)
                     /locus_tag="PLO65_08845"
     CDS             complement(<410..988)
                     /locus_tag="PLO65_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HRD28394.1"
                     /translation="MARDGAVYVCQACGAAQSKWAGQCPACSAWNSLVEEVTSRPPGA
                     LTPTKGPRSRGLTFEGLQSDSPAPPRIVTGVDEFDRVCGGGVVPGSAILVGGDPGVGK
                     STLLLQVVAQAALRGVACAYISGEEAIEQVRSRAGRMGLADAPVRLAAETSLRDVLDG
                     LKKERFDLVVIDSIQTMWSDAHEAGPGSVTQVR"
     gene            1150..1776
                     /locus_tag="PLO65_08850"
     CDS             1150..1776
                     /locus_tag="PLO65_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRD28395.1"
                     /translation="MSVVDGGGSSSGLAQRVQDILLRPAPTWEAIDAEPATVKGLYQG
                     YVMVLAAIGPVCGFLGGLLFHSFIFAPFTLVAALAGYVLGLAGVYVMALVIDGLAPSF
                     GGTSNRIQAFKVAAYSGTAAWVAGVFQLVPMLSFLGIVGLYSLYLLYVGLPRLMKVSA
                     DKAMVYTLVVVVLMIVIYAVIAMVVSMITLMGAAGAIGAAAIGAAAYN"
     gene            complement(1773..>2409)
                     /locus_tag="PLO65_08855"
     CDS             complement(1773..>2409)
                     /locus_tag="PLO65_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRD28396.1"
                     /translation="GVMLVMSHLACAGQPDHPMNAVQLARFRQARALFPGAPASLASS
                     GGIFLGRDYAFDQVRPGISLFGGGPHDRADGRIAAVATLEAAILQLRRVPAGETIGYG
                     AAFTAERELQVAILGAGYADGVPWSANPKGAVWFEGARRRMVGRISMDMIAVDLTGCE
                     TARPGALVEILGANLPVDDAAAAAGTTSYELLTRLSTRAKRRWIGAEPPAA"
     assembly_gap    2410..2461
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2462..2826)
                     /locus_tag="PLO65_08860"
                     /pseudo
     CDS             complement(2462..2826)
                     /locus_tag="PLO65_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919534.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
     gene            complement(2908..4395)
                     /locus_tag="PLO65_08865"
     CDS             complement(2908..4395)
                     /locus_tag="PLO65_08865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRD28397.1"
                     /translation="MALVPALDLRPVHDDPPLAVAPSNIEAEQALLGALLYDNAAYER
                     LSDRLQARHFYEPFHQRLFAALETHIRKGQLAEPILLADEFKKDPAFEELGGVRYLAD
                     LVDRAPPAANAPDYARAIYDLALRRDLIRIGGEITVEAQAHDADVSARDQIEAAEQKL
                     YTLAESGTSSTGFIPFADALTGAVQMAAEAYSRDGGLAGVSTGLTDLDKQLGGLHPSD
                     LLILAGRPSMGKTALATNIAFHVARNYAWEPQPDGTRKTVNGGVVAFFSLEMSAEQLA
                     MRLLADVSGVSSDRLRKGEIDASEFGRVRDGAIEVQEAPLFIDATGGLSISKLTARAR
                     RLKRQHGLDLIVVDYLQLVTTDGSKGDNRVQEVSAITGGLKALAKELGVPVIALSQLS
                     RQVENREDKRPQLSDLRESGSIEQDADVVMFVYREAYYLSRAEPREGTPEHMTWQEDL
                     EKINNIAEVIIGKQRHGPIGTVKLHFHGDTTKFGNLAREGRFDVR"
     gene            complement(4575..5159)
                     /gene="rplI"
                     /locus_tag="PLO65_08870"
     CDS             complement(4575..5159)
                     /gene="rplI"
                     /locus_tag="PLO65_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRD28398.1"
                     /translation="MKVILLERVEGRGALGDVVTVKDGFARNYLLPRHKALRATAANM
                     KVFDAQRAEIEARNAKAKDAAGEAGQALDGTSYILIRQAGETGQLYGSVSGRDVADMV
                     NAEGGKIDRSMVVLDKPIKTLGVHPVKVKLHAEVTITVNINIARSADEAERQARGENV
                     IASQFDEDAALAAESAADLLEGGAGSQEGFGDDR"
     gene            complement(5173..>5330)
                     /gene="rpsR"
                     /locus_tag="PLO65_08875"
     CDS             complement(5173..>5330)
                     /gene="rpsR"
                     /locus_tag="PLO65_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRD28399.1"
                     /translation="YKDVKLLQRYVSERGKIVPSRITAVSAKKQRELARAIKRARFLA
                     LLPYVVK"
BASE COUNT          712 a         1768 c         1879 g          844 t
ORIGIN      
        1 ccgagtccca gccgacaacg gcccggcgcg gggtgccgta ggccgacggg gccaccagcg
       61 cctggaactc caccagcacg gggcggctgc cctcgatgcc ggcgaacacc gccgccccgg
      121 ccgcgcgctc gccgccggcg ttgaggaaca gggccgacgg gttgatcacc tcggtcagac
      181 ccgcgtcgcc catctcgaac acgccgatct cgtcggtcgc gccgaagcgg ttcttggccc
      241 cgcgcaggat gcggaacgga tagccgcgct cgccctcgaa gctcagcact gcgtcgacca
      301 tgtgctcgac gacacggggg ccggcgatct ggccnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgtacctggg
      421 tcaccgagcc gggtccggcc tcgtgggcgt cgctccacat ggtctggatc gagtcgatca
      481 ccaccaggtc gaaccgctcc ttcttcagcc cgtccagcac gtcgcgcagc gaggtctctg
      541 ccgccagcct gaccggggcg tcggccaggc ccatgcggcc ggcgcggctg cgcacctgct
      601 cgatcgcctc ctcgccggag atgtaggcgc aggcgacgcc gcgcagcgcg gcctgggcga
      661 ccacctgcag caggagggtc gacttgccga ccccggggtc gccgccgacc aggatggcgc
      721 tgcccggcac cacgccgccg ccgcagacgc ggtcgaactc gtcgacgccg gtgacgatgc
      781 gcggcggggc agggctgtcg ctctgcaggc cctcgaaggt caggccgcgc gacctgggac
      841 ccttggttgg ggtcagggcg ccgggcggcc gcgaggtgac ctcttcgaca agactgttcc
      901 aggcgctgca ggccgggcac tggccggccc acttcgactg ggccgcgccg caggcctggc
      961 agacatagac ggcgccgtcg cgggccatgc ggtctcctga ttcgtggatc ggccgcacaa
     1021 taccggcaaa cgccggcccc cgcgcggccg ttcggcggca gtccgttcgc ggaaccgtgt
     1081 ttcgccatcg ccgccgagtt cgctaggctc gcctgtcagg ggagtcagga tcaacccacg
     1141 gagggtgtca tgagcgttgt cgatggaggc ggaagttcgt cggggcttgc ccagcgtgtg
     1201 caggacatcc tgctgaggcc cgccccgacc tgggaggcga tcgacgccga gccggcgacg
     1261 gtcaagggcc tgtaccaggg ctatgtgatg gtcctggcgg ccattgggcc ggtgtgcggc
     1321 ttcctgggag gcctgttgtt ccacagcttc atcttcgccc ccttcaccct ggtggccgcc
     1381 ctggcgggct acgtcctggg gttggcgggc gtctatgtca tggccctggt gatcgatggc
     1441 ctggccccca gcttcggcgg gacgagcaac cgcatccagg cgttcaaggt cgccgcctac
     1501 agcggcaccg cggcctgggt ggcgggggtc ttccagctgg tgccgatgct gtccttcctc
     1561 ggtatcgtcg gcctctacag cctctatctg ctctacgtcg gcctgccgcg gctgatgaag
     1621 gtctcggctg acaaggccat ggtctacacc ctggtcgtgg tggtgctgat gatcgtgatc
     1681 tacgccgtta tcgccatggt ggtcagcatg atcaccctga tgggcgcggc cggggcgatc
     1741 ggcgcggcgg cgatcggggc ggccgcctac aactaggcgg ccgggggctc tgcgccgatc
     1801 cagcgccgtt tggcgcgggt cgaaaggcgg gtgagcagct cgtaggatgt cgtgccggcc
     1861 gccgccgccg catcgtcgac cggcaggttg gcgccgagaa tctcgaccag cgcgccgggc
     1921 cgggccgttt cgcagccggt caggtcgacg gcgatcatgt ccatcgaaat gcgcccgacc
     1981 atccggcggc gcgcgccctc gaaccacacc gcgcccttcg gattggcgct ccagggcacg
     2041 ccgtcggcgt agccggcccc gaggatggcg acctgcagct cgcgttcggc cgtgaaggcc
     2101 gccccgtagc caatcgtctc accggccgga acgcggcgca gctgcaggat tgccgcctca
     2161 agcgtggcca ccgcggcgat gcggccgtcg gcccgatcgt gcggcccgcc gccgaacagg
     2221 ctgatgcccg gccgcacctg gtcaaaggcg tagtcccggc cgaggaagat gccgcccgag
     2281 ctggcgaggc tggccggcgc gccggggaac agtgcccggg cctggcggaa gcgggcgagc
     2341 tgaacggcgt tcatcgggtg gtctggctgg ccggcgcagg ccaggtggct catcaccagc
     2401 atcacgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 natgccggtg tcgatgtgga tcgccgccgg gccgtcccag gcgccggcct ggtcgaggct
     2521 gttgatcacc ggcgtcagcc ccgccgcgcg cagtgtctcc tgcgatcccg gcgtcgggcc
     2581 atcgaggacg tagatccggg cctcacggcc gccgagcgct cgtcgcagga ccacgccttc
     2641 ttcaagccgg gcgacgtgaa agctgcgcgc gccctcgcgc cagagccgca gggcggcctc
     2701 cggggcgcca atgccgtagc cgtcggcctt gatggccggc gccgcctcgc agccggccat
     2761 gtcccgcagc gccgcgtagt tgcgggccag ggcgtcgaga tcgatggtca gccgggcgac
     2821 ggtcatcgcc tgcggctaag gggcgaggtg gggcgggtca agccggcttg cgccaatcct
     2881 caccgggggc ggcgaaggaa tgagatttca ccgcacgtcg aaccggccct cgcgggcgag
     2941 gttgccgaac ttggtcgtgt cgccgtggaa gtgcagcttc accgtgccga tcggaccgtg
     3001 acgctgcttg ccgatgatca cctcggcgat gttgttgatc ttctccaggt cttcctgcca
     3061 ggtcatgtgc tcgggcgtgc cctcgcgcgg ttcggcgcgg ctcaggtagt aggcctcgcg
     3121 atagacgaac atgaccacgt cggcgtcctg ctcgatcgag cccgattccc gaaggtcgct
     3181 gagttgtggc cgcttgtcct cgcggttctc gacctgacgc gacagctggg acagggcgat
     3241 gacgggcacg cccagctcct tggccagcgc cttgaggccg ccggtgatgg ccgacacctc
     3301 ctgcacccgg ttgtcgccct tggagccgtc ggtggtcacc agctggaggt agtcgaccac
     3361 gatcaggtcc aggccgtgct ggcgcttcag gcggcgggcc cgggcggtca gcttggagat
     3421 cgacaggccg ccggtggcgt cgatgaacag cggcgcttcc tgcacctcga tggccccgtc
     3481 gcggacgcgg ccgaactcac tggcgtcgat ctcgcccttg cgcaggcgat ccgacgagac
     3541 gcccgacaca tcggccagca gacgcatggc cagctgctcg gcgctcattt ccagagagaa
     3601 gaaggccacc accccgccgt tgaccgtttt gcgcgtgccg tcgggttgcg gctcccaggc
     3661 atagttgcgg gcgacgtgga aggcgatgtt ggtggccagg gcggtcttcc ccatcgaggg
     3721 gcggccggcg aggatcaaca ggtccgaagg gtgaaggccg cccagctgct tgtcgaggtc
     3781 ggtcaggccg gtcgagaccc ccgccaggcc gccgtcgcgg ctgtaggcct ccgcggccat
     3841 ctgcacggcc ccggtcaggg cgtcggcgaa cgggatgaac ccggtcgagg acgtcccgct
     3901 ctcggccagg gtgtagagct tctgttcggc cgcctcgatc tggtcgcggg cggaaacgtc
     3961 ggcgtcgtgg gcctgggcct cgacggtgat ctcgccgccg atgcggatca gatcgcggcg
     4021 cagggccagg tcgtagatcg cgcgggcgta gtcgggagcg ttggccgccg gcggggcgcg
     4081 gtcgacgagg tcggcgaggt agcgcacccc gcccagctcc tcgaacgccg gatccttctt
     4141 gaactcgtcg gccagcagga tcggctcggc cagctggccc ttgcggatgt gcgtctccag
     4201 cgccgcgaac agccgctggt ggaacggttc gtagaagtgt cgcgcctgca gccggtcgct
     4261 cagccgttca taggcggcgt tgtcgtacag cagcgcgccc agcagcgcct gttcggcctc
     4321 gatgttcgac ggcgccacgg cgagcggcgg gtcatcatgg acgggacgga gatcaagcgc
     4381 gggaacgaga gccatggcct taggttaacg aagtcgctcg ggggccgcgc ggaccgatcc
     4441 gtcgcacccg cgattcatct gtgaggttca gtgtatgttc gcatggatgg ccgcggccgc
     4501 gcgtcacatt cgcggcacga aaaagggcgg cccggacaac cgggccgccc ttgtatttct
     4561 ggctcgcggg agacctagcg gtcgtcgccg aagccttctt gcgagccggc gccaccctcg
     4621 agcaggtcgg ccgccgactc ggccgccagg gccgcgtctt cgtcgaactg cgaagcgatg
     4681 acgttctcgc ccctggcctg gcgttcggct tcatcggccg agcgcgcgat gttgatgttc
     4741 accgtgatgg tgacctcggc atgcagcttc accttcaccg ggtggacgcc cagggtcttg
     4801 atcggcttgt ccaggacgac catcgagcgg tcgatcttgc cgccttcggc gttgaccatg
     4861 tcggccacgt cgcggccgga gaccgagccg tacagctggc cggtctcgcc cgcctgacgg
     4921 atcaggatgt aggacgtgcc gtcgagcgcc tggccggctt cgccggcggc gtccttggcc
     4981 ttggcgttgc gggcctcgat ctcggcgcgc tgggcgtcga agaccttcat gttggccgcg
     5041 gtggcgcgga gcgccttgtg ccggggcagg aggtagttgc gcgcgaagcc gtccttgacg
     5101 gtgacgacat cgccgagggc gccgcggccc tccacgcgtt cgagcaggat aactttcatc
     5161 ggtccggttc ccttacttca cgacgtaggg gagcagggcg aggaagcggg cgcgcttgat
     5221 ggcgcgggcc agctcacgct gcttcttggc cgagacggcg gtgatgcgcg aggggacgat
     5281 cttgccgcgc tcggaaacgt agcgctgcag gagcttgacg tccttgtagt
//
LOCUS       DAORNX010000176         5319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11472_length_5319_cov_9.251520, whole genome shotgun sequence.
ACCESSION   DAORNX010000176 DAORNX010000000
VERSION     DAORNX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11472_length_5319_cov_9.251520"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2179)
                     /gene="fusA"
                     /locus_tag="PLO65_08880"
     CDS             complement(101..2179)
                     /gene="fusA"
                     /locus_tag="PLO65_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRD28400.1"
                     /translation="MPRTTPIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAMWNGKRLNIIDTPGHVDFTIEVERSLRVLDGC
                     VAVLDGNQGVEPQTETVWRQADRYDVPRIVFVNKMDKTGADFDMCLATIRDRLGVKAV
                     PIQLPIGSEASLKGVIDLIRMKAVVWESEGLGANYHDEEIPADLKAKAEEARHYLVEN
                     AVELDDDAMEAYLGGEEPSEDVLKRCIRKAVLSGAFYPILCGSAFKNKGVQPLLDAVV
                     DYLPSPVDIPPTKGIDFKTEAEVERMASDDEPLSVLAFKIMDDPFVGSLTFCRVYSGK
                     IETGMGLLNSSRDKRERVGRMLLMHSNNREDIKEAYAGDIIALAGLKDTRTGDTLCDP
                     IKSPVILERMTFPEPVIEIAIEPKSKADQEKLGVALGKMVAEDPSFTVHTDQESGQTI
                     MKGMGELHLDIKVDILKRTYKVDANIGAPQVAYRESLGRKVDIDYTHKKQTGGTGQFA
                     RIKVTFEPGEPGSGFQFESSIVGGAVPKEFIPGVIKGLESSKENGLLAGFPVIDFKAT
                     LTDGAYHDVDSSVLAFEIAARAAFRELKEKGSPKLLEPIMKVEVLTPDEYLGDVIGDL
                     NSRRGQIQGTDQRGNAQVVTAFVPLANMFGYIGNLRGMSQGRAQFTMQYDHYEAVPQH
                     VADEVIKKYA"
     gene            complement(2263..2736)
                     /gene="rpsG"
                     /locus_tag="PLO65_08885"
     CDS             complement(2263..2736)
                     /gene="rpsG"
                     /locus_tag="PLO65_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRD28401.1"
                     /translation="MSRRRRAEKRQVLPDPKFGDLIVTKFMNYVMYEGKKAVAENIIY
                     GAFDILEAKRKDQGPLETFHSALDNVAPAIEVRSRRVGGATYQVPVEVRPDRRRALAI
                     RWLVTAARKRGENTMTEKLAAELLDASNNRGTAVKKREDTHKMAEANRAFSHYRW"
     gene            complement(2749..3120)
                     /gene="rpsL"
                     /locus_tag="PLO65_08890"
     CDS             complement(2749..3120)
                     /gene="rpsL"
                     /locus_tag="PLO65_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRD28402.1"
                     /translation="MPTVNQLIRKPRQPKPTRNKVPALKGCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTTGIEAVCYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGVL
                     DTQGVKDRRQRRSKYGAKRPK"
     gene            complement(3390..>5319)
                     /locus_tag="PLO65_08895"
     CDS             complement(3390..>5319)
                     /locus_tag="PLO65_08895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD28403.1"
                     /translation="APPPLSAFARLPAIEQAVISPDGARVALLGGPPGERTLFLAPVD
                     GQESVTVKLGQAWIRSIHWVGNDYLVVNFSMLDKGTNYSNGQSYAYQLNRNVVIDTQG
                     KLVASLLANTQWSRYAVSQPVLATLEGTRPSVVLLGLDLAVGARAGGVGTLIQATGND
                     LTATLWRVDVASGDASVVEKGNPWTAGWDLDIAGEPRARWDYDGDTDELSYFVRAKGK
                     AGWTALGPLEAGGAIPDYLGYSDPDDSVYLLDHDEDGVRLVRRGLTDGSVTPVPLNAP
                     ASDLRLRWDPRRIIPVAVVTESDRTSYQWLDSQLGAVQGKLSRAFAGRNVALASWSAD
                     RTRVVVRVDSPTSPPAWFLFDTRTNQASSLGEAYPELAGATLGTTSWITYKARDGLEI
                     PAYLTLPPGLAAGARPPLIVMPHGGPAARDSFEFDWWVQALATRGYAVLQPQFRGSAG
                     FGADFEDAGHRQWGGKMQEDLIDGVVALGQRGVVDAGRVCIVGASYGGYAAMFSAAVY
                     PSVYRCGVSVNGVSDLALLIGAKSRAFGRDTALVNALETMMGDPRNDPEIFRLASPTA
                     RVTRQAAPILLIHGEEDTTVPIQQSRSMQRALKANGRPADLVVLAGDDHHLSSTASRT
                     LMLESMFAFLDRWLPVTP"
BASE COUNT          775 a         1710 c         1856 g          978 t
ORIGIN      
        1 tgccgatgtt gcagtgcggc ttggtgcgtt cgaacttttc cttggccatt ttcttagctc
       61 ctggtccccc aatcggggtc cttacgtttg gggggtgggg ttaggcgtac ttcttgatca
      121 cttcgtcggc gacgtgttgc ggcaccgctt cgtagtggtc gtattgcatg gtgaactggg
      181 cgcggccctg cgacatgccc cgcaggttgc cgatgtagcc gaacatgttg gccagcggca
      241 cgaaggcggt cacgacctgg gcgttgccgc gctggtcggt gccctggatc tggccacggc
      301 ggctgttcag gtcgccgatg acgtcgccca gatattcgtc cggggtcaga acctcgacct
      361 tcatgatcgg ctcgagcagc ttgggcgagc ccttttcctt cagttcgcgg aacgcggcgc
      421 gggccgcgat ttcgaacgcc aggacgctgg agtcgacgtc gtgataggcc ccgtcggtca
      481 gggtcgcctt gaagtcgatc accgggaagc cggccagcag gccgttttcc ttggaagact
      541 cgaggccctt gatcacgccc gggatgaact ccttgggcac cgcgccgccg acgatggacg
      601 actcgaactg gaagcccgag cccggctcgc ccggctcgaa ggtcaccttg atgcgggcga
      661 actggcccgt accgccggtc tgcttcttgt gcgtgtagtc gatgtcgacc ttgcggccga
      721 ggctttcacg ataggccacc tgcggcgcgc cgatgttggc gtcaaccttg taggtccgct
      781 tcaggatgtc gaccttgatg tcgaggtgca gctcgcccat gcccttcatg atcgtctggc
      841 ccgactcctg gtcggtgtgg acggtgaagg acgggtcctc ggcgaccatc ttgccgagcg
      901 cgacgcccag cttttcctgg tcggccttgg acttcggctc gatggcgatc tcgatgacgg
      961 gttccgggaa cgtcatgcgc tccaggatca ccggcgactt gatcgggtcg cagagggtgt
     1021 cgcccgtgcg ggtgtccttc aggccagcca gggcgatgat gtcgccggcg taggcttcct
     1081 tgatgtcctc gcggttgttc gagtgcatga gcagcatgcg gccgacccgc tcgcgcttgt
     1141 cacgcgagga gttgagcagg cccatgccgg tctcgatctt gcccgagtag acgcggcaga
     1201 aggtcagcga accgacgaac gggtcgtcca tgatcttgaa cgccagcacc gacagcggct
     1261 cgtcgtccga ggccatgcgc tcgacttcgg cttcggtctt gaagtcgatg cccttggtcg
     1321 gagggatgtc caccggcgag ggaaggtagt cgacgacggc gtcgagcagg ggctggacgc
     1381 ccttgttctt gaaggccgag ccgcagagga tcggatagaa ggcgccgctc agcacggcct
     1441 tgcggatgca gcgcttcagc acgtcttccg aaggctcttc gccgcccagg taggcttcca
     1501 tggcgtcgtc gtcgagctcg acggcgtttt cgaccaggta gtggcgggct tcttccgcct
     1561 tggccttcag gtccgccggg atctcctcgt cgtggtagtt ggcgcccagg ccttcgcttt
     1621 cccagaccac cgccttcatg cggatcaggt cgatcacgcc cttgaggctc gcttccgacc
     1681 cgatcggcag ctggatcggc acggccttga cgcccagacg gtcgcggatg gtggccaggc
     1741 acatgtcgaa gtcggcgccg gtcttgtcca tcttgttgac gaacacgatg cgcggcacgt
     1801 cgtagcggtc ggcctgacgc cagaccgttt cggtctgcgg ctcgacgccc tggttgccgt
     1861 ccagaacggc cacgcagccg tccagcacgc gcagcgagcg ctcgacctcg atggtgaagt
     1921 cgacgtgccc gggggtgtcg atgatgttca ggcgcttgcc attccacatg gcggtcgtcg
     1981 cggccgacgt gatcgtgatg ccgcgctcct gctcctgctc catccagtcc atggtcgcgg
     2041 cgccgtcgtg gacttcgccg atcttatggg acttgccggt gtagtagagg atgcgttccg
     2101 tcgtcgtcgt cttgcccgcg tcgatgtggg ccatgattcc gaagttacgg tagtcttcga
     2161 tcggagttgt gcggggcata tcggtctgcc tgtcggccta taaaactgag cgcgggcggt
     2221 tttggagaac cgcccgcgcc ctggaaggtt gtgggctggg ggttaccagc ggtagtgcga
     2281 gaaggcccgg ttggcttcgg ccatcttgtg cgtgtcttcg cgcttcttga ccgcggtgcc
     2341 gcggttgttc gatgcgtcca gcagctcggc ggccagcttc tcggtcatgg tgttctcgcc
     2401 acgcttgcgc gcggcggtca ccagccagcg gatggccagg gcgcgacggc ggtccggacg
     2461 gacttccacg ggcacctggt aggtcgcgcc gccgacccgg cgggaccgga cctcgatggc
     2521 gggggcgacg ttgtcgagag ccgagtggaa ggtttccagg gggccctggt ccttgcgctt
     2581 ggcttccagg atgtcgaagg cgccgtagat gatgttctcg gcgacggctt tcttaccttc
     2641 gtacatcacg tagttcatga acttcgtgac aatcaggtcc ccgaacttgg gatcgggcag
     2701 aacctgacgc ttctctgcgc ggcggcggcg ggacattctt gctcttcctt acttcggacg
     2761 cttcgcgccg tacttcgaac ggcgttggcg acggtccttg acgccttgcg tgtcgagcac
     2821 gccgcgcagg atgtgataac ggacgccggg aaggtccttc acgcggccgc cgcggatgag
     2881 caccaccgag tgctcctgca gattgtggcc ttcgcccggg atgtagcaca cggcttcgat
     2941 gccggtggtc agacggacct tggcgacctt acgcagagcc gagttcggct tcttcggggt
     3001 cgtcgtgtag acgcgcgtgc agacgccgcg acgttggggg cagcccttga gggccggcac
     3061 cttgttccgc gtgggcttcg gctggcgagg cttgcggatg agctggttga ctgtaggcat
     3121 tcgctctttt cgtgctcgtg gaggcgtgtg cctttcggcc gaggcgcctc ggggtccttg
     3181 ttgatcgttc cggcgttccg gacagccctt ccgaacgcga aaactcgccg gcacgcgctc
     3241 tcatgcgttc cggcgggcct ggcggtcctt ggcgggacat ccgctgatcg ggacagaccc
     3301 ggatggaccg gccccgaaag aggggcggta aatactggcg aagggcccca aggtcaaggc
     3361 tttggaaagg gttgcagcgc ggccgccgtt cagggcgtca cgggcagcca gcgatcgagg
     3421 aaggcgaaca tcgactccag catcagggtg cggctggcgg tgctcgacag gtggtggtcg
     3481 tcgccggcca gaaccacgag gtccgccggc cggccgttcg ccttcagcgc ccgctgcatc
     3541 gaccgcgact gctggatcgg cacggtggtg tcctcctcac cgtggatcag caggataggc
     3601 gcggcctgcc gggtgacccg ggccgtcggc gaggccagac ggaagatctc cgggtcgttg
     3661 cgcgggtcgc ccatcatggt ctcgagggca ttgaccaggg cggtgtcacg gccgaaggcg
     3721 cgcgacttgg ccccgatcag cagcgccagg tcggagaccc cgttgaccga cacgccacag
     3781 cggtagaccg acggatagac ggcggcgctg aacatcgctg cgtagccgcc gtagctggcc
     3841 ccgacgatgc agacgcggcc ggcgtcgacc acgccgcgtt gacccagcgc cacaacgcca
     3901 tcgatcaggt cctcctgcat ctttccgccc cactgcctgt ggccggcgtc ctcgaagtcg
     3961 gcgccgaacc ccgcggagcc gcggaactgc ggctgcagca cggcgtagcc ccgggtcgcc
     4021 agggcctgga cccaccagtc gaactcgaag ctgtcgcggg ccgccggacc accgtgcggc
     4081 atgacgatca gcggcggccg ggcgccggcc gcgaggcccg gcggcagcgt cagataggcc
     4141 gggatctcca gaccgtcacg ggccttgtag gtgatccagc tggtggtccc cagggtcgcg
     4201 ccagccagtt cgggataggc ctcgccgagc gacgacgcct ggttggtgcg ggtgtcgaac
     4261 aggaaccagg ccggcggact ggtcggcgag tcgaccctca ccacgacccg ggtgcggtcg
     4321 gccgaccagc tggccagggc cacgttacgc ccggcgaagg cccgcgacag cttgccctgg
     4381 acggcgccca gctgggaatc cagccactgg taggaggtcc ggtccgattc cgtgacgacg
     4441 gccaccggga tgatccggcg cggatcccac cgcagccgca ggtcgctggc cggggcgttc
     4501 agcggcacgg gagtgaccga cccgtccgtc agtccgcggc gcaccagacg cacgccgtcc
     4561 tcgtcgtggt cgagcaggta gaccgagtca tcgggatcgg aatagccgag gtagtccggg
     4621 atggcgccgc ccgcctcgag cggccccagc gcggtccagc ccgccttgcc cttggcccgg
     4681 acgaagtagc tcagttcgtc ggtgtcgccg tcgtagtccc accgcgcgcg aggttcgccg
     4741 gcgatgtcga ggtcccagcc cgccgtccag ggattgccct tttcgacgac cgaggcgtca
     4801 ccgctggcca cgtcgacccg ccacagcgtc gcggtcaggt cgttgccggt ggcctggatc
     4861 agggtgccca cgccgcccgc tcgcgcgcca acggccaggt cgaggcccag cagcaccacg
     4921 gacgggcgag tcccctccag ggtcgccagc accggctggg acactgcgta gcgggaccac
     4981 tgggtgttgg cgagcagcga ggcgaccagc ttgccctgcg tgtcgatcac gacattgcgg
     5041 ttgagctggt aggcgtacga ctggccgttg gaatagttgg tgcccttgtc gagcatggag
     5101 aagttgacca ccagatagtc attgccgacc cagtggatgc tgcggatcca ggcctggccc
     5161 agcttgacgg tcaccgactc ctgcccgtcg acgggcgcga ggaacagggt ccgctcaccg
     5221 ggagggccgc ccagcagggc cacccgcgcc ccgtcgggcg agatcaccgc ctgctcgatc
     5281 gccggcaggc gggcgaaggc cgacaggggc ggcggggcg
//
LOCUS       DAORNX010000177         5298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11537_length_5298_cov_7.666794, whole genome shotgun sequence.
ACCESSION   DAORNX010000177 DAORNX010000000
VERSION     DAORNX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_5298_cov_7.666794"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLO65_08900"
     CDS             <1..1318
                     /locus_tag="PLO65_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD28404.1"
                     /translation="VSGREVMIVANDATVKGGTYYPMTVKKHLRAQEVALQNRLPCVY
                     LVDSGGANLPHQSEVFPDRDHFGRIFYNQARMSAEGIAQIACVMGSCTAGGAYVPAMS
                     DETVIVRGAGDESQGTIFLAGPPLVKAATGEVISATDLGGADVHGRRSGVVDHVAAND
                     EHALEIVRSIVGNLNTVKRVDMDVREPKAPLFDPAELYGVVPADVRAPYDVHEVIARI
                     VDGSEFDEFKPLYGTTLVTGFARIWGYPVAILANNGVLFSESALKAAHFIELACKRRI
                     PLVFLQNISGFMVGGKYEAGGIAKDGAKMVTAVASANVPKFTVLIGGSFGAGNYGMCG
                     RAYSPRFLFTWPNSRISVMGGEQAASVLATVHRDADKWTKDEEEAFKAPVRQKYEDEG
                     NPYHATSRMWDDGIIDPAQTRDVLGLAISASLNAPIPETTFGVFRM"
     gene            1493..2332
                     /locus_tag="PLO65_08905"
     CDS             1493..2332
                     /locus_tag="PLO65_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD28405.1"
                     /translation="MTNPIADPLVEVPDTDALSSLVELDASPSGVAVVTINRADKKNA
                     FNAEVIAALAQHFETLRDADGVRIVFLRGAGGAFSAGADLDWMKSAAGFTRDENHEDA
                     MALAEMLLALRELPQLTVALVDGPAFGGGAGLVAACDYAVATKGAKFAFSEVKLGLVA
                     ATISPHVVDAIGPRRAKSLFATGRMFDAAFAEKIGLVDELVAGAADLDDVMERMADEI
                     MACAPGAVAKSKALVDDVAGVPIDDELLDLTARLIADQRTSEEGQEGVAAFLEKRKPG
                     WAL"
     gene            2351..3289
                     /locus_tag="PLO65_08910"
     CDS             2351..3289
                     /locus_tag="PLO65_08910"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRD28406.1"
                     /translation="MSHELEAAAADAAGGFFSRKPRHSLPVGTPCPNCETPLEGPWCH
                     QCGQKGEDFHRSIGHLVTEAFEGLTHFDGRFWQTMPDLAWRPGRLTRAYLDGRRAPQI
                     PPFRLFLVVVLLVFFAGGLGPSRTNVITAKQGDGQVLSAPGIKIGVTENTPPPPFEID
                     TGQGAFDHWMESHVRAALQNPDAFGHSLHAWGHRLAVLALPIAAALLGLLFALPSRRF
                     YLFDHLIFSMHSLSFQGLLLSLVFVINLAPGKVGDILLLAMPVHLFLHMRGTYGTGVF
                     GTLGRMALLFIGSFIGAGVIMVILMFLGLNDVAAAH"
     gene            3331..5232
                     /locus_tag="PLO65_08915"
     CDS             3331..5232
                     /locus_tag="PLO65_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRD28407.1"
                     /translation="MLKSLLIANRGEIARRIIRTARRLGVRTVAVYSEADADSPHVAE
                     ADEAVLIGPAPARESYLVVEKILAAAKQTGAEAIHPGYGFLSENAAFAQAVKDAGLVW
                     VGPPPAAINAMGLKDAAKSLMIAAGVPCTPGYLGDDQSEARLETEAAAIGYPVLIKAV
                     AGGGGKGMRKVDAAGDFLAALASCRREAAASFGDDRVLIEKYVTRPRHIEVQVFGDRH
                     GNVVHLFERDCSLQRRHQKVIEEAPAPGMDAATRAAVCDAAVKAARAVNYEGAGTVEF
                     IADASEGLRADRIWFMEMNTRLQVEHPVTEMITGQDLVEWQLRVASGEPLPLMQDQIT
                     LTGWAMEARLYAENPATGFLPSTGPLTHFRLPEEGIRVDSAVEEGGEVSPHYDPMIAK
                     LIAHGGTREAAAEALAEACASVEVWPVKTNAAFLARCASHPDFVAGDVDTGFIERRLE
                     ALLGDGRPSAVVAGVAATALVELQSRDEEPTPWSALDLAAGFRLNGPPRRSMTLICGA
                     TELEGALLDVDLHPEDLLVLDENEVVVFEYGETFAFRRPTADAVAHGGESDGAVISPM
                     PGKVVSVSVKAGDTVSKGQTLLVLEAMKMEHALAAPFDGVVAELTAVAGGQVSEGVVL
                     ARLEAEVRE"
BASE COUNT          829 a         1820 c         1855 g          794 t
ORIGIN      
        1 cgtgtccggc cgcgaggtga tgatcgtggc caacgacgcc acggtgaagg gcggcaccta
       61 ctacccgatg acggtcaaga agcacctgcg ggcgcaggag gtcgcgctgc agaaccggct
      121 gccctgcgtc tatctggtgg acagcggcgg ggccaacctg ccgcaccagt ccgaggtctt
      181 ccccgaccgc gaccacttcg gccgcatctt ctacaaccag gcccggatga gcgccgaggg
      241 catcgcccag atcgcctgcg tgatgggcag ctgcaccgcc ggcggggcct atgtcccggc
      301 catgagcgac gagacggtca tcgtccgcgg cgcgggcgac gagagccagg gcaccatctt
      361 cctcgccggc cccccgctgg tgaaggccgc cacgggcgag gtcatcagcg ccacggatct
      421 cggcggcgcc gacgtgcacg gccgccgcag cggcgtggtc gaccatgtcg ccgccaatga
      481 cgagcacgcg ctggagatcg tccgctcgat cgtcggcaac ctgaacaccg tcaagcgcgt
      541 cgacatggat gtgcgtgaac cgaaggcccc gctgttcgac ccggccgagc tctacggcgt
      601 ggtcccggcc gacgtgcgcg cgccctacga cgtgcacgag gtcatcgccc gcatcgtcga
      661 tggcagcgag ttcgacgagt tcaagccgct ctatggaacc acgctggtca cgggcttcgc
      721 gcgcatctgg ggctacccgg tggccattct ggccaacaac ggcgtgctgt tcagcgaaag
      781 cgccctgaag gcggcccact tcatcgagct ggcctgcaag cgcaggatcc cgctggtgtt
      841 cctgcagaac atctccggct tcatggtcgg cgggaagtac gaggccggcg gcatcgccaa
      901 ggacggggcc aagatggtca ccgccgtcgc cagcgccaac gtgcccaagt tcaccgtgct
      961 gatcggcggc agcttcgggg ccggcaacta cggcatgtgc ggccgggcct attcgccccg
     1021 cttcctgttc acctggccca acagccgcat cagcgtgatg ggcggcgagc aggccgccag
     1081 cgtgctggcc accgtccacc gcgacgccga caaatggacc aaggacgagg aagaggcctt
     1141 caaggctccc gtccggcaga agtacgagga cgagggcaac ccctaccacg ccacctcgcg
     1201 gatgtgggac gacggcatca tcgacccggc ccagacgaga gacgtcctcg gactggccat
     1261 ttccgcaagc ttgaatgccc ccatcccgga gacgactttt ggcgtattca ggatgtagcg
     1321 acgccccctc tcccggaggg agggggcggg gcccgacgca acgccgcggg agggtgaggg
     1381 gttacggcct taccgaacgc ccgccgacgc cttacaccct catcctccca cgcccgtcgg
     1441 gcgcgggccc ctccttctcc ctccgggaga agggacttca ggacaggccc tcatgaccaa
     1501 tcccatcgcc gatcccctcg tcgaagtgcc cgacaccgac gccctcagct cgctggtcga
     1561 gctcgacgcc agcccgtcag gcgtggcggt cgtcacgatc aaccgggcgg acaagaagaa
     1621 cgccttcaac gccgaggtca tcgcggccct ggcgcagcat ttcgagaccc ttcgcgacgc
     1681 cgacggcgtg cgcatcgtgt tcctgcgggg ggcgggcggg gcgttcagcg ccggcgccga
     1741 cctcgactgg atgaagtccg ccgccggctt cacccgcgac gagaaccatg aggacgccat
     1801 ggccctggcc gagatgctgc tggccctgcg cgagctgccg cagctgacgg tggccctggt
     1861 cgacggcccg gccttcggcg gcggcgccgg cctggtcgcg gcctgcgact acgcggtggc
     1921 gacgaagggc gcgaagttcg ccttctccga ggtcaagctg ggcctggtcg cggcgaccat
     1981 cagcccgcat gtggtcgacg ccatcggccc gcgccgggcc aagagcctgt tcgccaccgg
     2041 ccggatgttc gacgccgcct tcgccgagaa gatcggcctg gtcgacgaac tggtcgccgg
     2101 ggccgccgac ctcgacgacg tcatggagcg catggccgac gagatcatgg cctgcgcccc
     2161 cggcgccgtg gccaagtcca aggccctggt cgacgacgtc gccggcgtgc cgatcgatga
     2221 cgagctgctc gatctgacgg cccggctgat cgccgaccag cgcaccagcg aggaaggtca
     2281 ggagggcgtc gccgccttcc tcgaaaagcg caaacccggc tgggcgctct aggaccaggg
     2341 gagagggggc gtgtctcacg agctcgaagc ggcggcggcc gacgcggcgg gcgggttctt
     2401 ttcccgcaag ccccggcaca gcctgccggt gggaacgccc tgtcccaact gcgagacgcc
     2461 gctggagggg ccctggtgcc accagtgcgg ccagaagggc gaggacttcc atcgctcgat
     2521 aggtcatctg gtgaccgagg cgttcgaggg cctgacccat ttcgacggcc ggttctggca
     2581 gaccatgccg gacctggcct ggcggccggg caggctgacc cgcgcctatc tcgacggccg
     2641 gcgggcgccg cagataccgc cgttccgtct gttcctggtc gtggtcctgc tggtgttctt
     2701 cgccggaggg cttggaccgt cccggaccaa tgtcattacc gccaagcagg gcgatggcca
     2761 ggtcctgtcg gccccgggca tcaagatcgg ggtgactgaa aacaccccgc cgccgccgtt
     2821 cgagatcgat accggccagg gcgcgttcga ccactggatg gagtcgcatg tccgggcggc
     2881 gctgcagaac cccgacgcct tcggtcacag cctgcatgcc tggggtcacc ggctggcggt
     2941 cctggccctg ccgatcgcgg cggccttgct cggcctgctg ttcgcccttc ccagccgacg
     3001 gttctatctg ttcgaccacc tgatcttttc gatgcacagc ctgtcgttcc agggattgct
     3061 gctgagtctg gtgttcgtga tcaacctggc cccgggcaag gtcggcgaca tcctcctgct
     3121 ggccatgccg gtgcatctgt tcctgcacat gcgcggaacc tacgggacgg gcgtgttcgg
     3181 cactctcggg cggatggccc tgctgttcat cggctcgttc atcggcgcgg gtgtgatcat
     3241 ggtcatcctg atgttcctgg gcctcaacga cgtggccgcg gcgcattgag cgcggttcgc
     3301 attcgccggc cattccgcta ggagaggggc atgctgaaat ccctgctcat cgccaaccgg
     3361 ggcgagatcg cccgtcggat catccgcacg gcccgccggc tgggcgtgcg cacggtcgcc
     3421 gtctattccg aggccgacgc cgactcgccg catgtcgccg aggctgacga ggcggtgctg
     3481 atcggcccgg ccccggcgcg cgaaagctat ctggtcgtcg agaagatcct cgccgccgcg
     3541 aaacagacgg gcgcggaggc gatccacccc ggctacggct tcctgtccga gaacgccgcc
     3601 ttcgcccagg cggtcaagga tgcgggcctg gtctgggtcg gcccgccgcc ggccgcgatc
     3661 aacgccatgg gcctgaagga cgcggccaag agcctgatga tcgccgccgg ggtgccctgc
     3721 acgcccggct acctcggcga cgaccagagc gaggcgcggc tggagacgga ggccgcggcc
     3781 atcggctatc ccgtgctgat caaggccgtg gccggcggcg gcggcaaggg catgcgcaag
     3841 gtcgacgcgg ccggcgactt cctggccgcc cttgccagct gccgccgcga ggccgccgcc
     3901 agcttcggcg acgaccgggt gctgatcgag aaatacgtca cccgcccgcg tcacatcgag
     3961 gtgcaggtgt tcggcgaccg gcacggcaac gtcgtccacc tgttcgagcg cgactgctcc
     4021 ctgcagcggc gccaccagaa ggtcatcgag gaggcccccg cacccggcat ggacgccgcc
     4081 acccgcgcgg ccgtctgcga cgcggcggtc aaggccgcga gggccgtcaa ctacgagggc
     4141 gccggcacgg tcgagttcat cgccgacgcc agcgagggcc tgcgcgccga ccgcatctgg
     4201 ttcatggaga tgaacacccg gctgcaggtc gagcacccgg tgaccgaaat gatcaccggc
     4261 caggacctgg tcgaatggca gctgcgcgtc gccagcggcg aaccgctgcc gctgatgcag
     4321 gaccagatca ccctgaccgg ctgggccatg gaggcgcggc tctatgcgga gaacccggcg
     4381 acgggtttcc tgccgagcac ggggccgctg acgcatttcc ggctgccgga agagggtatc
     4441 cgagtcgaca gtgcggtcga ggaaggcggc gaggtctcgc ctcactacga cccgatgatc
     4501 gccaagctga tcgcgcacgg cggcacgcgg gaagcggccg ccgaggcgct cgccgaggcc
     4561 tgcgcctcgg ttgaagtctg gccggtgaag accaacgccg ccttccttgc gaggtgcgcg
     4621 agccatccgg actttgtcgc gggcgacgtc gatacgggct tcatcgaacg gcggctggag
     4681 gccctgctgg gcgatggccg accgtcggcg gtcgtggccg gcgtcgccgc cacggcgctg
     4741 gtcgagttac agtcccgcga cgaggagccg acgccctggt cggcgctgga ccttgccgcc
     4801 ggtttccgcc tgaacggacc gccgcgccgt agcatgacgc tgatctgcgg cgccacggag
     4861 cttgaggggg cattgctgga cgtcgacctc catcccgagg accttcttgt cctcgacgag
     4921 aacgaggtcg tggtgttcga atacggcgag accttcgcgt ttcgccgccc taccgccgac
     4981 gccgtcgccc atggcggcga gtccgatggc gcggtcatct cgcccatgcc gggcaaggtc
     5041 gtctcggtct cggtcaaggc cggcgacacg gtcagcaaag gccagaccct gctggtgctt
     5101 gaggcgatga agatggaaca cgccctggcc gcgccgttcg acggcgtggt cgccgagctg
     5161 accgccgtcg ccggcgggca ggtcagcgag ggggtggtgc tggcgaggct ggaggccgag
     5221 gtcagggagt agggagtagg gagtagggag tagggagtag ggagtaggga gtagggagta
     5281 gggagtaggg agtaggga
//
LOCUS       DAORNX010000178         5278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11592_length_5278_cov_3.649052, whole genome shotgun sequence.
ACCESSION   DAORNX010000178 DAORNX010000000
VERSION     DAORNX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11592_length_5278_cov_3.649052"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>934
                     /locus_tag="PLO65_08920"
     CDS             <1..>934
                     /locus_tag="PLO65_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD28408.1"
                     /translation="MTPWGFREHLRATRPDALNRGFVVVQEGRFVGVGTPATLLTARR
                     APAPATQDPSALLIQRMSGEVLRHLEGVADFTTRLARQNLPPDAQACVRAIGETGDDL
                     TSLMRRAVDLHEANLGVLTFHPHPCALRTFMDTVDARWKGRAAASGLTLLTAYDGDPE
                     DVADLDEARLLQVFDALIFRALSETRRGAIEATLKVRPTADGLILEGRVRDAGGEVSP
                     RRLARIFEPVMPTDDQTDGLAVSLGMALASRITAAAGGVIRAEPNPGAGVTVVFEFIA
                     RQARAAAAPGPAEGAPAQAAHILIVDDNATNRMVA"
     assembly_gap    935..1164
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1267..1749)
                     /locus_tag="PLO65_08925"
     CDS             complement(1267..1749)
                     /locus_tag="PLO65_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28409.1"
                     /translation="MTAPVILAATALAGCATLEIDRNAGRSGAPFHDVRPYLVISQAD
                     DCSRTASVLSLPDMTTTYFVRPRPGLGSSTLSATFANGQLTTFNQEASVDPTGLINTL
                     AGLVGLPGGEGAARGGGEKGVESAGGDRGGRPAACPVATWIFVAGPDGALTPAMPPIK
                     "
     gene            complement(1873..1949)
                     /locus_tag="PLO65_08930"
     tRNA            complement(1873..1949)
                     /locus_tag="PLO65_08930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1913..1915),aa:Met,seq:cat)
     gene            2072..3100
                     /locus_tag="PLO65_08935"
     CDS             2072..3100
                     /locus_tag="PLO65_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD28410.1"
                     /translation="MSVLHLLGSGGDGGAETYFVDLVAALHRAGVAEACAIRRHPARA
                     DALAAAGVVAQSFGFGGPLDLLTRPRVAALGRRLDTRVMVAWMNRAARHTPAGPWTRI
                     GRLGGYYSLKYYRGFDHLVGNTRDIVDWVIGQGWPASQASYIPNFAGGPDDSAPVARA
                     SLDTPEGVPLLLGMGRLHDAKAHDVSLKALTQVPEAWLWIAGAGALEDKLKAMAVALG
                     VADRVRFLGWRQDAAALYRAADVCVFPSRFEPLGNVVIQAWANGLPVVAANSQGPGAL
                     VRDGEDGFLVPIEDADALADRVKAVLADPMLRIKLIQNGHARVEAEFAEAAVVAEWRH
                     LFSRLGEG"
     gene            3100..4935
                     /locus_tag="PLO65_08940"
     CDS             3100..4935
                     /locus_tag="PLO65_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRD28411.1"
                     /translation="MCGIAGILGVTDPEAAERDVRAMIERIAHRGPNGIRVESGPGWC
                     VAHARLSIIDLEGGWQPLHAAGSTIIGNGEIYNYLELAEEFQLGPKLATGSDFEPLLH
                     LYAQEGEKAFERLRGMYAFCLIGGDGRTWLVRDGFGIKPLYTDQTETGFRFASEPRAL
                     TMDGRQAPPSASQVEQLLSLGYLIDGPIGSWADTQAPGFATQRLPNGDLGDMLVIDWQ
                     SAGPSPRSAHPREGGDPGVFDDIGSRRRGSEKTWVPAFAGMSGEEELLAALDAVLEDS
                     VKVHQRSDVPYGLFLSGGIDSTAIAALMSRLNERPVTAYTCGFDAPEARDERGQAERV
                     ARALNLDWRETTFGEEDFWRIAPQVANFLDDPTTDYACLPTYKLAEAARGTLTVVLSG
                     EGGDELFGGYGRYRRALRPGWLGGRAAEPQTDAAFLKDKGAGVLARWREASEPPAGLT
                     RLQQAQYADIVTWLPNDLLLKLDRMLMAHGLEGRTPFLDKEVAAFAFNLPDRMKVRGK
                     YGKYILRKWLERHCPAAEPWAKKKGFTVPVASWIAPRAGDLGPRIADVKAVAEVCDVE
                     AVRAVFTDPAQAHNRWPLMFFALWSRIHLEGAEPSAALASLLGDA"
     gene            4947..>5278
                     /locus_tag="PLO65_08945"
     CDS             4947..>5278
                     /locus_tag="PLO65_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRD28412.1"
                     /translation="MRPAPLVLAALILAACGGQPSGAQTAGQPLETRPPNAEGQTPAF
                     PGQTRAPLVSAGVTFDVVTVATGLDHPWAMAWLPDGRMLVTERAGRLRIVAKDGTLSP
                     PVEGLPEV"
BASE COUNT          722 a         1798 c         1793 g          735 t
ORIGIN      
        1 atgaccccct ggggctttcg cgaacatctt cgggcgaccc gccccgacgc cctgaaccgg
       61 ggcttcgtcg tcgtccagga gggacgcttc gtcggcgtcg gcacgccggc gacgctgctc
      121 actgcccgcc gcgccccggc gccggcgacg caggatccgt ccgccctgct gatccagcgg
      181 atgagcggcg aggtgctgcg ccacctcgag ggcgtggccg atttcaccac ccgcctggcg
      241 cgccagaacc tgccgccgga cgcccaggcc tgcgtgcggg cgatcggcga gaccggcgac
      301 gacctgacct cgctgatgcg ccgcgccgtc gacctgcacg aggccaacct gggggtgctg
      361 accttccacc cgcatccctg cgcgctgcgc accttcatgg acaccgtcga tgcgcgctgg
      421 aaaggccgcg ccgccgccag cggcctgacc ctgctgaccg cctacgacgg cgaccccgag
      481 gatgtcgccg atctcgacga ggcgcgcctg ctgcaggtgt tcgacgccct gatcttccgc
      541 gccctgagcg aaacgcgacg cggggccatc gaggccacgc tcaaggtgcg gccgaccgcc
      601 gacggcctga tcctcgaggg ccgtgtccgc gacgccggcg gcgaggtcag cccccgacgc
      661 ctggcgcgca tcttcgaacc ggtgatgccg accgacgacc agaccgacgg cctggcggtg
      721 agcctgggca tggccctggc cagccgcatc accgccgccg ccggcggggt tatccgcgcc
      781 gagcccaacc ccggcgcggg cgtgaccgtg gtgttcgagt tcatcgcccg tcaggccagg
      841 gccgccgccg cgccgggccc cgccgaaggc gcgccggccc aggccgccca catcctgatt
      901 gtcgacgaca acgccaccaa ccgcatggtg gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnngtcgaa aagccgatca aggccgagcg catgctggcc
     1201 gcgttgcagg ccgccctcga cggggcggac gaccagtcgg ccgcggcctg aacgcccggg
     1261 aacgtcctat ttgatcggcg gcatggccgg cgtcagggcg ccgtccggtc cggccacgaa
     1321 gatccatgtc gccacgggac aggccgcggg gcggccgccc cggtcgccgc cggccgattc
     1381 gacgcccttc tcgccgcccc ctcgggccgc gccctccccg cccggcagcc cgaccaggcc
     1441 ggccagggtg ttgatcaggc ccgtgggatc gacgctggct tcctggttga aggtcgtcag
     1501 ctggccattg gcgaaggtcg cgctcagcgt gctgctcccc agccctggcc ggggacggac
     1561 gaaataggtc gtggtcatgt ccggcaggga caggacgctg gcggtccggc tgcagtcgtc
     1621 ggcctggctg atcaccagat agggacggac atcgtggaac ggcgccccgc tcctgcccgc
     1681 attccggtcg atctccagag tggcgcagcc ggccagggcg gtcgcggcca gaatcacagg
     1741 cgcggtcaag attcgcatcg ccttccccca tgccgcgacg gcggcccgcc accatccctt
     1801 ggcgcatgga ggaattcaag atcgcgggct gtcgttcgcc gggcgggatc gagtcgaacc
     1861 ggcggttccg ggtggtagct gggggcggga tcgaaccgcc gacctgtggg ttatgaatcc
     1921 accgctctaa ccatctgagc tacccagccg cacgcgacgg aacatcagtc cgggaccgcg
     1981 agaggcggct tatacgggtg ccggcgtttc gcttcaagag catcacatcc gccttgggaa
     2041 acggcgtccc gaccgctagg gtcgccgccg catgagcgtg cttcatctgc ttgggtccgg
     2101 gggcgacggc ggcgccgaga cctacttcgt cgacctcgtc gcggccctgc atcgcgcggg
     2161 ggtggccgag gcctgtgcga tccgtcgcca tccggcccgg gccgacgccc tggccgccgc
     2221 gggggtggtc gcgcagagct tcgggttcgg cggtccgctc gaccttctca cccgtccgcg
     2281 cgtggcggcg ctgggtcgcc ggctggacac gcgggtgatg gtcgcctgga tgaaccgcgc
     2341 ggcccggcat acgccggccg ggccctggac ccgcatcggc cggcttgggg gctactacag
     2401 cctgaaatac tatcgcggct tcgaccacct ggtcggcaac acccgcgaca tcgtcgactg
     2461 ggtgatcggt cagggctggc cggcgagcca ggccagctac atccccaatt tcgccggcgg
     2521 accggacgac tccgcgccgg tggcccgcgc cagcctggac acgccggagg gcgtgccgct
     2581 gctgctcggc atgggccggc tgcacgacgc caaggcccat gacgtcagcc tgaaggcgct
     2641 gacgcaggtc ccagaggcct ggctgtggat cgccggcgcc ggggcgctgg aggacaagct
     2701 gaaggcgatg gccgtcgcgc tgggcgtcgc cgaccgcgtg cgcttcctcg gctggcggca
     2761 ggacgcggcg gccctctacc gtgcggccga cgtctgcgtg ttcccctcgc ggttcgagcc
     2821 gctcggcaat gtggtcatcc aggcctgggc caacggcctg ccggtcgtcg cggccaacag
     2881 ccaggggccg ggcgccctgg tccgcgatgg cgaggacggc ttcctcgttc cgatcgagga
     2941 cgccgacgcc ctggccgacc gggtcaaggc cgtgctggcc gacccgatgc tgcgcatcaa
     3001 gctgatccag aacggccacg cgcgggtcga ggccgagttc gccgaggccg cggtggtcgc
     3061 cgaatggcgc cacctgttct cgcggctggg cgaaggctga tgtgcggcat cgccggcatc
     3121 ctgggcgtga ctgaccctga agccgccgag cgcgacgttc gggcgatgat cgagcggatc
     3181 gcccaccgtg gtcccaacgg catccgggtg gagagcgggc ccggctggtg cgtggcccac
     3241 gcgcggctgt cgatcatcga cctggagggc ggctggcagc cgctgcacgc ggccggctcg
     3301 acgatcatcg gcaacggcga gatctacaac tacctggagc tggcggagga attccagctg
     3361 gggccgaaac tggcgacagg gtctgacttc gagcccctgc tgcacctcta cgcccaggag
     3421 ggcgagaagg ccttcgagcg gctgcggggg atgtacgcgt tttgcctgat cggcggggac
     3481 ggacggacct ggctggtcag ggacgggttc gggatcaagc cgctgtacac ggaccagacc
     3541 gagaccggct tccgatttgc ctccgaaccc agagcgctga cgatggatgg ccgccaggcg
     3601 ccgccgtccg ccagtcaggt cgaacagcta ttgtcgctcg gctacctcat cgacgggccg
     3661 attggatcct gggcggatac tcaggctccg ggtttcgcga ctcagcgcct gccaaatggc
     3721 gatcttggcg acatgctggt tatcgattgg cagtccgctg gcccctcccc ccgttccgct
     3781 catccccgcg aaggcgggga cccaggcgtt tttgatgaca tcggctcacg tcggcggggt
     3841 tctgaaaaaa cctgggtccc cgccttcgcg gggatgagcg gagaagaaga attgctggcc
     3901 gcgttggacg ccgttctcga agacagcgtg aaggtccacc agcggtcgga cgtgccctac
     3961 ggcctgttcc tgtcgggcgg gatcgacagc acggccatcg ccgcgctgat gagtcggctc
     4021 aacgagcggc cagtgaccgc ctacacctgc ggtttcgatg ccccggaggc ccgcgatgag
     4081 cgtggccagg cggaacgggt cgcgcgcgcg ctgaatctcg actggcgcga gacgaccttc
     4141 ggcgaggaag acttctggcg gatcgcgccg caggtggcga acttcctcga tgatccgacg
     4201 accgactacg cctgcctgcc gacctacaag ctcgccgagg cggccagggg cacgctgacg
     4261 gtggtgctca gcggcgaggg cggggacgag ctgttcggcg gctacggccg atatcgccgg
     4321 gcgctgcggc ccgggtggct tggcgggcgg gcggcggagc cgcagaccga cgcggccttc
     4381 ctgaaggaca aaggggcggg ggtcctcgcc cgctggcggg aggcgtcgga gccacccgcc
     4441 ggcctcaccc gcctgcagca ggcccagtac gccgacatcg tcacctggct gcccaatgat
     4501 ctgctgctga agctcgaccg gatgctgatg gcccatggcc tcgagggccg cacaccgttc
     4561 ctcgacaagg aggtcgcggc cttcgcgttc aacctgccgg accggatgaa ggtgcgcggc
     4621 aaatacggca aatacatcct gcgcaaatgg ctggagcggc actgccccgc cgccgagccc
     4681 tgggcgaaaa agaaggggtt caccgtcccg gtggccagct ggatcgcgcc gcgcgccggg
     4741 gacctgggcc cccgcatcgc cgatgtgaag gccgtggccg aggtctgtga tgtcgaggcc
     4801 gtgcgcgcgg tgttcaccga cccggcccag gcccacaacc gctggccgct gatgttcttc
     4861 gccctctggt cgcggattca tctggagggg gcggaaccgt ccgctgccct cgcctctttg
     4921 ctcggcgacg cctgaggaga ttcgccatgc gccccgcccc gctcgtcctc gccgccctga
     4981 tcctcgccgc ctgcggcgga caaccgtccg gggcccagac cgccggccag ccgctggaga
     5041 cccggccgcc caacgccgaa gggcagacac cggctttccc gggccagacc cgcgcgccgc
     5101 tggtcagcgc cggtgtgacc ttcgacgtcg tgaccgtagc gaccgggctg gatcacccat
     5161 gggccatggc ctggctgccc gacggccgta tgctggtcac cgaacgcgcc ggccggctgc
     5221 gcatcgtggc gaaggacggg accctgtcgc cgcctgtcga gggcctgccg gaggtcga
//
LOCUS       DAORNX010000179         5259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11638_length_5259_cov_7.227517, whole genome shotgun sequence.
ACCESSION   DAORNX010000179 DAORNX010000000
VERSION     DAORNX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_5259_cov_7.227517"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..839)
                     /locus_tag="PLO65_08950"
     CDS             complement(231..839)
                     /locus_tag="PLO65_08950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRD28413.1"
                     /translation="MARTGPDFLLEQACVGPVCGVDEAGRGPWAGPVSAAAVILDPRK
                     APRGLDDSKKLSPERRQFLEVEIKARAVAWCVAFATVEEIDRLNILQATGLAMRRAVE
                     GLAVAPAFALVDGNYRFNLPCEIRTVVGGDGLSASIAAASILAKTARDRLMVELDAVH
                     PGYGFASHKGYHAPVHVEALRTLGPSPIHRMTWAPIRELLGN"
     gene            complement(839..1426)
                     /locus_tag="PLO65_08955"
     CDS             complement(839..1426)
                     /locus_tag="PLO65_08955"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRD28414.1"
                     /translation="MTDSLTRCAWPGATDAQYLAYHDEEWGVPEYDSRALWEKLVLDG
                     FQAGLAWITILRKRDAFRLAFDNFDPEKVARYGEADRARLMADAGIVRSNAKIDAAIG
                     GAKIWLEMRDNGEDFSEFLWSFVDDKPVQNRWTHTKQVPVTSPEAEAMSKALKKRGFK
                     FVGPVIVYAFMQAVGMVNDHLEDCFRHGPVRDMAR"
     gene            complement(1471..2352)
                     /locus_tag="PLO65_08960"
     CDS             complement(1471..2352)
                     /locus_tag="PLO65_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRD28415.1"
                     /translation="MNDAPTHALLRDRALVSVTGPDWRSFLQGLLSQDVEGLAVGELR
                     FGALLSPQGRLLFDLFILGRQDGCWLDVASDRREALIARLAMYRLRAKVEIAALDGVV
                     SALFSDHSREGGNPSDRSTAAEQAWVPASAGTNGYCQDPRLPALGLRGYGVVAPPGER
                     ADAAAYDRHRLALGVPDTADFGEDSTYPIEADFDLLNGIDFKKGCFVGQETTSRMKRR
                     GQIRSRMLPITFDGPPPAFGAEILNGELRAGQVLSGVPGRAMALLRLDRLEGPTLTAD
                     GRTVSVGRPAWLESPES"
     gene            2413..3396
                     /locus_tag="PLO65_08965"
     CDS             2413..3396
                     /locus_tag="PLO65_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRD28416.1"
                     /translation="MAAKPFPILFIGPSRIGDAVLASGLIRKLADEAPGARFTIAVGP
                     AAAPLFAQTPGLDALIVMEKRKGLGHWFSLWNRVRHTRWSLVVDMRGSAISSFLRRDR
                     RAVRRKPVGEPMHKVIEAASLLTLQDDPPAPFLYTSDEIETRATELLGEGGPILAMGP
                     AANWLGKTWPIERFARVAIELLDEGGPMAGGRLLILGGPDDARYVEPLARALPRDRVI
                     DLTGQADLLTAYACLKRARLFVGNDSGLMHLAAAAGAPTIGLFGPSDDRLYAPWGPHT
                     RVVRGPREFEQFKAIDPGLNQTIGHMMDLPVDWVVEAAKALLEATAPARDL"
     gene            3506..4840
                     /locus_tag="PLO65_08970"
     CDS             3506..4840
                     /locus_tag="PLO65_08970"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRD28417.1"
                     /translation="MTQTYDLIIRGGEVVNHAGRGVTDIGIRNGKFAFVGDLSQASAE
                     AVFDAAGLTVLPGVIDSQVHFREPGLEWKEDLETGANAAVLGGVTAVFEMPNTEPTTT
                     DPDALADKLRRASGRMSCDHAFYVGGTHDNARFLGELERLPGCCGIKVFMGASTGTLL
                     IKDDAGVKEVLRHINRRAAFHSEDEYRLEERRPLARTGDWTSHPEVRDAESAIRSTRR
                     LVGLARETGKRIHVLHVTTAEEIEFLALNRDVASVELTPQHLTLEGPEAYERLKGYAQ
                     MNPPIREARHRAALWVGLSTGVADVMGSDHAPHTAEEKARPYPASPSGMPGVQTLVPI
                     MLTHVAEGRLTLERFVDLTSHGPNRVFGLAEKGRIAEGFDADLTLVDLKARRIIRHAD
                     QASRVGWTPFDGFEAKGWPVATIVRGITVMRDDEIVAHGQGRPVRFGETLSA"
     gene            4918..>5259
                     /locus_tag="PLO65_08975"
     CDS             4918..>5259
                     /locus_tag="PLO65_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HRD28418.1"
                     /translation="MDGNTQLTTAPFRDARYAPRRLDIERRPDGTLVLANPTPVDGTF
                     TTTLDALVHWGAAAPERVWLAERAGDGWRTVDYATGLRQVSALAGGLKGLGLKRGDCL
                     LILARNTVDHAL"
BASE COUNT          794 a         1820 c         1885 g          760 t
ORIGIN      
        1 tcggcggcgg gtcggaggag atcatgcgcg acctggcggc ccggcagatg gggatatgag
       61 accccattcc ctccccggag cgtagcgagg gggaggggga ccgccggagg cggtggaggg
      121 gcggcgggaa cgcgagcggc cccatcctga attcaggtca ttccggagag ggcggcgcag
      181 cccctccacc acgctccgcg tggtcccccc tccccgactt cgcggggagg ttagttgccg
      241 agcaactccc tgatcggcgc ccaggtcatg cggtggatcg ggctcgggcc gagcgtccgc
      301 agggcctcga catggaccgg cgcgtgatag cccttgtggc tggcgaaacc gtagccggga
      361 tggaccgcgt ccaactcgac catcagccgg tcgcgggcgg tcttggcgag gatcgaggcc
      421 gcggcgatcg aggcgctgag gccgtcgccg ccgaccaccg tcctgatctc gcagggcagg
      481 ttgaagcgat agttgccgtc caccagggcg aaggccgggg ccaccgccag gccctcgacc
      541 gcgcggcgca tggccaggcc cgtggcctgc aggatgttca ggcgatcgat ctcctcgacc
      601 gtggcgaagg ccacgcacca ggccacggcc cgggccttga tctcgacctc gaggaactgg
      661 cgcctttcgg ggctgagttt cttggagtcg tcgagccccc ggggggcctt gcgcggatcg
      721 aggatcaccg ccgctgcgct gaccggtccc gcccaggggc cgcggccggc ctcatcgacg
      781 ccgcagaccg ggccgacaca ggcttgctca agcaggaagt cggggccggt cctcgccatc
      841 agcgcgccat atcgcggaca gggccgtggc ggaagcagtc ctcgaggtgg tcgttgacca
      901 tgccgacggc ctgcatgaag gcgtagacga tcaccgggcc gacgaacttg aagccgcgct
      961 tcttcagcgc cttgctcatg gcctcggcct caggcgaggt caccggcacc tgtttcgtgt
     1021 gcgtccagcg gttctggacc ggcttgtcgt cgacgaagct ccagaggaat tcgctgaagt
     1081 cctcgccgtt gtcgcgcatc tccagccaga tcttcgcccc gccgatggcc gcgtcgatct
     1141 tggcgttcga gcgcacgatg ccggcgtcgg ccatcagccg ggcgcggtcc gcctcgccgt
     1201 agcgggcgac cttttccggg tcgaagttgt cgaacgccag gcggaaggcg tcacgcttgc
     1261 gcaggatggt gatccaggcg agcccggcct gaaacccgtc cagcacaagc ttctcccaga
     1321 gggcgcggga atcgtattcc ggcacgcccc actcctcgtc gtggtaggcg aggtattgcg
     1381 cgtccgtggc cccgggccag gcgcagcggg tcagtgagtc ggtcatcggg tcagtctagc
     1441 ggctgatcag cgacacggca gccggtccgc tcacgactcc ggggattcaa gccacgccgg
     1501 ccgcccgacg ctcaccgtcc gtccgtccgc ggtcagtgtg gggccctcca ggcgatcgag
     1561 ccgcagcagg gccatggccc ggcccgggac gcccgacagc acctgacccg cgcgcagttc
     1621 gccgttgagg atctcggcgc cgaaggccgg cggggggccg tcgaaggtga tcggcagcat
     1681 gcggctcctg atttggccgc gtcgtttcat ccggctggtg gtctcttggc cgacgaaaca
     1741 gcccttcttg aagtcgatgc cgttcagaag gtcgaaatcg gcctcgatcg gataggtcga
     1801 gtcctcgccg aagtcggccg tgtcgggcac gccgagggcg aggcggtggc ggtcgtaggc
     1861 ggcggcgtcc gcgcgctcgc ccggcggcgc gacaacgccg tagccgcgca ggccgagggc
     1921 gggcaggcgg gggtcctgac aatatccatt cgtccccgcg gaggcgggga cccaggcctg
     1981 ctcagcggcg gtggaccgat cgcttgggtt cccgccttcg cgggaatggt cggagaagag
     2041 ggcagagacc accccgtcca gcgccgcgat ctcgaccttc gcccgcagcc ggtacatcgc
     2101 cagccgggcg atcagggctt cgcgccggtc ggacgccacg tcgagccagc agccgtcctg
     2161 gcggccgagg atgaacaggt cgaacagcag ccggccctgg ggcgagagca gggcgccgaa
     2221 gcgcagttcg ccaacggcca ggccttcgac atcctggctc aacaggccct gcaggaagga
     2281 ccgccagtcg ggcccggtga cggagaccag ggcgcggtcg cgcagcaggg cgtgggtcgg
     2341 ggcgtcgttc atcgtcccga tgtggccttt cgcgcgctgt aacgccagtg tccgccagtc
     2401 taaggtgcgc ggatggcggc taagcccttc ccgattctgt tcattggccc gagccggatc
     2461 ggcgacgccg tgctggcgtc cggcctgatc aggaagctcg ccgatgaggc gccgggggcg
     2521 cggttcacca tcgccgtggg gccggcggcc gcgccgctgt tcgcgcagac gccgggactc
     2581 gacgccctga tcgtcatgga gaagcgcaag ggtctcggcc actggttttc gctgtggaac
     2641 cgggtgcgcc acacgcgctg gagcctggtc gtcgacatgc gcggttcggc gatcagcagc
     2701 tttctgaggc gtgaccgccg ggcggtgcgc cgcaagcctg tcggcgagcc catgcacaag
     2761 gtgatcgagg cggccagcct gctcacgctc caggacgatc cgccggcgcc cttcctctac
     2821 acctccgacg agatcgagac gcgcgcgacc gagttgctcg gcgagggcgg tccgatcctg
     2881 gccatgggcc cggcggccaa ctggctgggc aagacctggc cgatcgagcg gttcgcccgg
     2941 gtggccatcg aactgctcga cgagggcgga ccgatggccg gcggccggct gttgatcctc
     3001 gggggaccgg acgatgcgcg ctacgtcgag ccgctggccc gcgccctgcc gcgcgaccgg
     3061 gtcatcgacc tgaccggcca ggccgacctg ctgaccgcct acgcctgtct caagcgggcg
     3121 cggctgttcg tcggcaacga ttcagggctg atgcatctgg ccgccgccgc cggcgcgccg
     3181 accatcggcc tgttcggccc atccgacgac cggctctacg cgccctgggg gccgcacacc
     3241 cgcgtcgtgc gcgggccgcg ggagttcgag cagttcaagg ccatcgatcc cggcctcaac
     3301 cagaccatcg gccacatgat ggacctgccc gtcgactggg tggtcgaggc ggcgaaggcg
     3361 ctgcttgaag ctaccgcgcc ggcgcgcgac ctctaggatc cgctcatccc ggcgaaggcc
     3421 gggacccagg ctttttctgg aaagcgcggc gccaaacgct aaaacacctg ggtcccggcc
     3481 ttcgccggga tgagcggaat tcgaaatgac ccagacctac gacctgatca tccgcggcgg
     3541 cgaggtggtg aaccacgcgg ggcgcggcgt caccgacatc ggcatccgca acggcaagtt
     3601 cgcgttcgtc ggcgacctgt cccaggcctc ggcggaggcg gtgttcgacg ccgcgggcct
     3661 gaccgtgttg cccggggtga tcgacagcca ggtgcatttc cgcgagccgg ggctggagtg
     3721 gaaggaagac ctcgagaccg gagccaacgc cgccgtgctg ggcggggtca ccgccgtgtt
     3781 cgagatgccc aataccgaac cgaccaccac cgacccggac gccctggccg acaagctccg
     3841 gcgcgcgtcc gggcggatga gctgcgacca cgccttctat gtcggcggca cgcacgacaa
     3901 cgcgcggttc ctcggcgaac tggagcggct gcccggctgc tgcggcatca aggtgttcat
     3961 gggcgcctcg accggcaccc tgctgatcaa ggacgacgcg ggggtcaagg aggtgctgcg
     4021 ccatatcaac cgccgcgcgg ccttccactc cgaggatgaa taccggctcg aggagcgtcg
     4081 cccgctggcc cggaccggcg actggaccag ccacccggag gtgcgcgacg ccgagagcgc
     4141 gatccgctcg acccggcggc tcgtcggcct ggcccgcgag accggcaagc gcattcatgt
     4201 gctgcatgtg accacggcgg aagagatcga attcctggcc ctgaaccggg acgtggccag
     4261 cgtcgaactg acgccgcaac acctgaccct cgaggggccc gaggcctatg agcggctcaa
     4321 gggctacgcc cagatgaacc cgccgatccg cgaggcgcgt caccgcgccg ccctgtgggt
     4381 cggcctgtcg accggggtgg ccgacgtcat gggctccgac cacgcgcccc ataccgccga
     4441 ggagaaggcg cggccctatc cggcctcgcc gtcgggcatg cccggggtgc agaccctggt
     4501 gccgatcatg ctgacccacg tggccgaggg ccggctgacg ctggagcggt tcgtcgacct
     4561 gaccagccat ggccccaacc gggtgttcgg cctggccgag aagggccgca tcgccgaggg
     4621 cttcgacgcc gacctgaccc tcgtcgacct caaggcccgc cgcattatcc gccacgccga
     4681 ccaggccagc cgggtcggct ggactccgtt cgacggcttc gaggcgaaag gctggcccgt
     4741 ggcgaccatc gtgcgcggca tcacggtcat gcgcgatgac gagatcgtgg cgcacgggca
     4801 aggacgcccg gtgcggttcg gggagacgct ttcggcctaa cgagatatgc gagaagcctc
     4861 cccagcggag ctggggagga ttgatttgtc gcgccatggc gtccgataac cgcgcgcatg
     4921 gacggcaaca cccagctcac caccgccccc tttcgtgacg cccgctacgc gccgcgccgg
     4981 ctcgacatcg agcggcggcc ggacggcacg ctcgtgctgg ccaatccgac gccggtcgac
     5041 gggaccttca ccaccacgct cgacgccctt gtccactggg gcgcggccgc gccggagcgg
     5101 gtctggctgg cggagcgggc cggggacggc tggcggacgg tcgactacgc cacggggctg
     5161 aggcaggtct cggcgctggc cggcgggctg aaagggctgg ggctgaagcg tggcgactgc
     5221 ctgcttatcc tggcccgcaa cacggtcgac cacgcgctg
//
LOCUS       DAORNX010000180         5229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11728_length_5229_cov_7.980093, whole genome shotgun sequence.
ACCESSION   DAORNX010000180 DAORNX010000000
VERSION     DAORNX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11728_length_5229_cov_7.980093"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLO65_08980"
     CDS             <1..221
                     /locus_tag="PLO65_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28419.1"
                     /translation="RPAATPAERAPRNLAPARLRALSLDGRLYEEATAALAPDVDPAL
                     AGALGTDLWSRWRLRLDIGRGRLVLRPK"
     gene            complement(276..1070)
                     /locus_tag="PLO65_08985"
     CDS             complement(276..1070)
                     /locus_tag="PLO65_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRD28420.1"
                     /translation="MLRKVTLLAAALLLVGGPALASSTAHEPEPVAFSFEGPFGQFDA
                     AQLQRGYKVYREVCAACHSMNLLSFRNLGDRGGPFYDPKYPNANDNPVVKALATDTQI
                     GDLDSETGDAIQRPGVPADRFPAPFPNVIAAKASNGGLAPPDLSLMAKAREGGPEYIA
                     SLLQGFGPPEHGVTLAPGQHYNRYFPGGVIGMAPPLAPDKVTFDDGTKSTVRQQAEDV
                     AAFLMWAAEPKMEERKQMGFSVLIFLVLFSGLLYASYRRVWRNESH"
     gene            complement(1077..2360)
                     /locus_tag="PLO65_08990"
     CDS             complement(1077..2360)
                     /locus_tag="PLO65_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HRD28421.1"
                     /translation="MSGHSTYQPNNGFSRWLDARLPVGRLVWDSFVDYPTPRNLNYWW
                     TFGGILSLCLGVQLITGIVLAMHYTPSAAAAFTSVEHIMRDVNYGWLLRYMHANGASM
                     FFIAVYIHMLRGVYYGSYKAPREVLWLLGCVIYLLMMATAFMGYVLPWGQMSFHGAVV
                     ITNLLGAFPMVGDAITTLLWGGFAVDEPTLKRFFSLHYLLPFMIAGVVVLHVWALHVV
                     GQNNPTGVEPKSKADTLPFTPYATVKDGFAMSLFLILFAVFVFYMPNALGHADNYIPA
                     NPLVTPSHIVPEWYFLPFYAILRAIPDKLMGVLAMFGAIACIFALPWLDTSKVKSMRY
                     RPQAKLYFFIFLVACIILGFCGAKLPDDPVIPGLKTFMLVDSDLNSFVWLSRISTLYY
                     FAYFLVVMPLLGLFEKPLPTPETISSPVLGGDKKD"
     gene            complement(2366..2914)
                     /gene="petA"
                     /locus_tag="PLO65_08995"
     CDS             complement(2366..2914)
                     /gene="petA"
                     /locus_tag="PLO65_08995"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRD28422.1"
                     /translation="MAHTAPDATTAVAGDDPSRRDFIHIAAITAAAGGGIAAIWPFVA
                     QMNPAADTLALASVEYDLTKIEPAQQVTINWRGKPLFIRNRTPEQIAAAVKDDHADLR
                     DPQTDAERHKEGKAQWLVLIGSCTHLGCVPTVGGGDWGGWFCPCHGSHYDTSGRIRKG
                     PAPKNLAVPEYAFLSDTKVKIG"
     gene            3147..4019
                     /gene="hemF"
                     /locus_tag="PLO65_09000"
     CDS             3147..4019
                     /gene="hemF"
                     /locus_tag="PLO65_09000"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRD28423.1"
                     /translation="MTDAALLDDRKARARAWFESLRDRILDAFEALEDEAPSDLFPGA
                     PGRFVRKPWVREAGGGGVMSMMHGRLFEKVGVHTSMVFGTFTPEMAANMPGAKEDPRF
                     FATGVSLIAHMASPRVPAVHMNTRFICTTRGWFGGGGDLTPLLAFQRDQAFPDTVAFH
                     AAMKAACDAHDPAWHAKYKAWCDEYFFLPHRNEPRGTGGIFYDHHDSGDWDRDFAFTR
                     DVGEAFLDIYPKIVRGRMAEPWTAEEREEQLVRRGRYVEFNLLYDRGTTFGLKTGGNV
                     ESILSSMPPAVKWP"
     gene            4270..4983
                     /locus_tag="PLO65_09005"
     CDS             4270..4983
                     /locus_tag="PLO65_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28424.1"
                     /translation="MIDISEPAQRGAFDLYGPIHKGLRMAHANLLIRLGAAELEDRRA
                     LGGLLAHLRDHLDLCAEHLRHEQTVIHTALAPFAPGAVAEIEVLHRAHLAGLPDLHGR
                     IDALETAAPAGLRKAGRALYLAFSRFVASDLTQMAHEEEVLLPLLQAAFDDAELIALK
                     TRIVLSMTPDVGAGMLRMIIPAARHRERLSLLRAVGAAAQQDIFEALLREVVRPSLAD
                     REWRRLADELLSDVLVEAA"
     gene            complement(5017..>5229)
                     /locus_tag="PLO65_09010"
     CDS             complement(5017..>5229)
                     /locus_tag="PLO65_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRD28425.1"
                     /translation="WRALIPLADTWTPPAFPLTGEDVVNAGVPRGPMVGQVLREIEDW
                     WIDLDFIDDKLAAVERLKAVAQGMAY"
BASE COUNT          846 a         1735 c         1830 g          818 t
ORIGIN      
        1 tgcgccccgc ggcaaccccg gccgagcggg cgccgcgcaa tctggccccc gcgcggctgc
       61 gggccctgtc cctcgacggc cggctctacg aggaggccac ggcggccctg gcgccggatg
      121 tcgatcccgc cctggccggg gccctgggca cggacctgtg gtcacgctgg cgcctgcgtc
      181 tggatatcgg ccggggccgg ctggtgctcc gccccaaatg acgaaggccc gggagcgatc
      241 ccgggccttc aatccgtact gggtcggtgg agcgcctagt ggctctcgtt ccgccacacc
      301 cgccggtagc tggcgtagag caggcccgag aacaggacca ggaagatcag caccgagaag
      361 cccatctgct tgcgctcctc catcttcggc tcggcggccc acatcaggaa ggcggccacg
      421 tcctcggcct gctgccggac cgtcgacttc gtgccgtcgt caaacgtcac cttgtccggc
      481 gccaggggcg gggccatgcc gataacccca ccggggaaat acctgttgta gtgctgcccc
      541 ggagccaggg tcacgccgtg ttccggcgga ccgaagccct gcagcaggct ggcgatgtat
      601 tccgggcccc cttcgcgcgc cttggccatc agcgacaggt cgggcggggc gaggccgccg
      661 ttgctggcct tggccgcgat cacgttcggg aacggcgcgg ggaagcggtc ggccggcacg
      721 ccgggccgct ggatcgcgtc gccggtctcg ctgtcgagat cgccgatctg ggtgtccgtc
      781 gccagggcct ttacgaccgg gttgtcgttg gcgttcgggt acttcgggtc atagaacggc
      841 ccgccccggt cgccgagatt gcggaacgac agcaggttca tgctgtggca ggccgcgcag
      901 acctcgcggt agaccttgta gccccgctgg agctgggcgg cgtcgaactg gccgaacggc
      961 ccctcgaagc tgaaggcgac cggctcgggc tcgtgagccg tgctggaggc gagggccggt
     1021 ccgccgacga ggagcagcgc cgccgcgagc agtgtgactt tgcgcagcat cggggctcag
     1081 tccttcttgt cgccgccgag aaccggcgag gagatggttt caggcgtcgg cagcggcttc
     1141 tcgaacaggc ccagcagcgg catcaccacc aggaagtagg cgaagtagta gagcgtcgag
     1201 atgcgcgaca gccagacgaa gctgttgaga tcggaatcga ccagcatgaa ggtcttcagg
     1261 ccggggatca ccgggtcgtc cggcagcttg gcgccgcaga agccgaggat gatgcaggcc
     1321 accaggaaga tgaagaagta cagcttggcc tggggccggt agcgcatcga cttgaccttg
     1381 gacgtgtcga gccacggcag ggcgaagatg caggcgatgg cgccgaacat cgccagcacg
     1441 cccatcagct tgtccggaat ggcccgcagg atcgcgtaga acggcaggaa gtaccattcg
     1501 ggcacgatgt gagacggggt caccagcggg ttggccggga tgtagttgtc cgcgtggccc
     1561 agggcgttgg gcatgtagaa cacgaacacc gcgaagagga tcaggaacag gctcatcgcg
     1621 aagccgtcct tcaccgtcgc gtagggcgtg aagggcaggg tgtcggcctt cgacttgggc
     1681 tcgacgccgg tcgggttgtt ctggccgacc acgtgcaggg cccagacgtg cagcacgacc
     1741 acgccggcga tcatgaacgg cagcaggtag tgcagcgaga agaagcgctt cagcgtcggc
     1801 tcgtcgacgg cgaagccgcc ccagagcagg gtggtgatgg cgtcgccgac catcgggaag
     1861 gcgcccagca ggttggtgat caccaccgcg ccgtggaagc tcatctgtcc ccagggcagg
     1921 acgtaaccca tgaaggcggt ggccatcatc agcaggtaga tcacgcagcc gagcagccag
     1981 agcacctcgc gcggggcctt gtacgacccg tagtacacgc cgcgcagcat gtggatgtag
     2041 acggcgatga agaacatcga ggcgccgttg gcgtgcatgt agcgcagcag ccagccgtag
     2101 ttcacgtcgc gcatgatgtg ctcgacggag gtgaaggcgg cggcggcgct gggcgtgtag
     2161 tgcatcgcca gcacgatgcc ggtgatcagc tggacgccca ggcacagcga caggatgccg
     2221 ccgaaggtcc accagtagtt caggttccgc ggcgtcggat agtcgacgaa gctgtcccag
     2281 accagacggc cgacgggcag gcgcgcgtca agccagcgag agaaaccgtt gttcggttgg
     2341 taggtcgaat gtccgctcat ctaaatcagc cgatcttgac cttggtgtcg gagaggaagg
     2401 cgtattcggg caccgcgagg ttcttcggcg ccgggccttt gcggatacga ccggaggtgt
     2461 cgtagtgcga gccgtggcag gggcagaacc agccgcccca gtccccgccg ccgaccgtcg
     2521 gcacgcagcc gaggtgggtg caggagccga tcagcaccag ccactgggcc ttgccctcct
     2581 tgtggcgctc ggcgtcggtc tgcgggtcgc gcaggtcggc atggtcgtcc ttgaccgccg
     2641 cggcgatctg ctcgggcgtc cggttgcgga tgaacagcgg cttgccgcgc cagttgatgg
     2701 tcacctgctg ggccggttcg atcttggtca gatcgtattc gacgctggcc agggccaggg
     2761 tgtcggcggc gggattcatc tgggcgacga atggccagat cgcggcgatg ccgccgccgg
     2821 cggcggcggt gatcgcggcg atatggatga agtcgcgcct ggagggatcg tctccggcga
     2881 ccgcggtcgt tgcgtcaggg gcggtgtggg ccacctttag atcacccttc ataaaggtcg
     2941 cgccgacagg gcgcggctcg caaatgctcg cggaacatcc tcactgtcgc gcgcggctgg
     3001 acccataatg ggttgagccc cgccccggtg atgccccgtg gcaggaccga ttagtgcgcg
     3061 tctcgtcctt tttcaaccga ccatgtcggg aatatggata tccgggccgc atggacgcgc
     3121 cgatgagagg ctgtaaaagc cggttcatga ccgacgcagc ccttctcgac gaccgcaaag
     3181 cccgggcccg ggcatggttc gaatcacttc gcgaccggat tctcgacgcc ttcgaggcgc
     3241 tcgaggacga agccccgtcc gacctgtttc ccggcgctcc ggggcggttc gtgcgcaaac
     3301 cctgggtccg cgaggccggc ggcggcggcg tgatgtcgat gatgcacggc cggctgttcg
     3361 agaaggtcgg ggtgcatacc tcgatggtgt tcgggacctt cactccggaa atggccgcca
     3421 acatgcccgg cgcgaaggag gatccgcggt tcttcgccac aggggtcagc ctgatcgccc
     3481 acatggccag cccgcgcgtg ccggccgtgc acatgaacac ccgcttcatc tgcacgacca
     3541 ggggctggtt cggcggcggc ggcgacctga cgcccctgct ggccttccag cgcgaccagg
     3601 ccttccccga caccgtcgcc ttccacgccg ccatgaaggc cgcctgcgac gcccacgacc
     3661 ccgcctggca cgcgaaatac aaggcctggt gcgacgagta tttcttcctg ccgcaccgca
     3721 atgagccgcg cggcacgggc ggcatcttct acgatcacca cgacagcggc gactgggacc
     3781 gcgacttcgc cttcacgcgc gacgtcggcg aggccttcct cgacatctat ccgaagatcg
     3841 tccgcggccg catggccgag ccctggacgg cggaggagcg tgaggaacag ctggtccgcc
     3901 gcggccgcta cgtcgagttc aacctgctct atgaccgcgg cacgaccttc ggcctgaaga
     3961 ccggcggcaa cgtcgagtcg atcctcagct cgatgccgcc ggcggtgaag tggccgtagg
     4021 cgactgaagg accgagctcc gcggagccgt cggaacatta cgggacttta tgttgaacgc
     4081 cgttatcgcg gcgcatattg gcgtttctga cagtcttggt tcgccccgtg gcgacgactg
     4141 aacatgacga aacggcaatg aaatgaaact atactacgaa tatacctcgc ctgtccgtcg
     4201 cggccgccgt cggccggaca ccgcccccac ccgcgcccgc gcgcgggcgg cctgaggcgc
     4261 gagggggcga tgatcgacat ctccgagccg gcgcagaggg gcgccttcga cctctatggc
     4321 ccgatccaca agggcctgcg gatggcgcac gccaacctgc tgatccggct gggcgcggcg
     4381 gagctcgagg accgccgggc cctgggcggc ctgctggctc atctgcgcga ccatctggac
     4441 ctgtgcgccg agcacctgcg ccacgaacag acggtgatcc acaccgccct cgccccgttc
     4501 gcgcccggcg ccgtggccga gatcgaggtc ctgcatcggg cccacctcgc cggcctgccg
     4561 gacctgcacg gccggatcga cgcgctggag accgctgctc cggccggcct gcgcaaggct
     4621 ggccgcgccc tctacctggc cttctcccgc ttcgtcgcct cggacctgac ccagatggcc
     4681 cacgaggaag aggtgctgct gccgctgctc caggccgcgt tcgacgacgc cgagttgatc
     4741 gcgctgaaga cccgcatcgt gctcagcatg acccctgacg tcggcgcggg catgctgcgg
     4801 atgatcatcc cggcggcgcg ccaccgcgag cggctttcac tgctgcgggc ggtgggggcg
     4861 gcggcccagc aggacatctt cgaggccctg ctgcgcgagg tcgtccggcc cagcctggcc
     4921 gaccgggagt ggcgccgcct ggcggatgaa ctgctgagcg acgtgctggt cgaggcggcc
     4981 tgaccgcgga attgcgccgc tcccggcggg cgttgctcag taggccatgc cctgggcgac
     5041 ggccttgagt cgctccaccg ccgccagctt gtcgtcgatg aagtcgagat cgatccacca
     5101 gtcctcgatc tcgcgcagca cctggccgac catcggcccg cgcggcacgc cggcgttgac
     5161 cacgtcctcg cccgtcaggg ggaaggccgg cggcgtccag gtgtcggcca gcgggatcag
     5221 ggcccgcca
//
LOCUS       DAORNX010000181         5209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11794_length_5209_cov_8.144936, whole genome shotgun sequence.
ACCESSION   DAORNX010000181 DAORNX010000000
VERSION     DAORNX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11794_length_5209_cov_8.144936"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLO65_09015"
     CDS             complement(<1..956)
                     /locus_tag="PLO65_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRD28426.1"
                     /translation="MLPIEEILPRLGEALADGSCAVLVAPPGAGKTTRVPLFLKDQPW
                     SAGGKLIVLEPRRLAARAAAARMASSLGEAVGETIGFRVRLQSKVSARTRIEVVTEGV
                     FTRMIQDDPGLDGVAAVLFDEFHERSLDADLGLALARDTQQLLREDLRLLVMSATLDG
                     AAVARLLGDAPVIESQGRAFPVETRHVGRNPALRLEEQVGRAVERALAEESGGILVFL
                     PGQGEILRTRDWLAERMRRTDVDLAPLYGAMDPADQDRAISASPPGRRKVVLATSIAE
                     TSLTIEGVRVVIDAGLSRVPRYDPASGLTRLETVRVSRAAAD"
     gene            1089..2660
                     /locus_tag="PLO65_09020"
     CDS             1089..2660
                     /locus_tag="PLO65_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRD28427.1"
                     /translation="MSADAAPRGNRLFLKKSIEQVQHESNTHQLKRTLGAWNLVLLGI
                     GCIIGAGIYVMTGNAAANFAGPAVMLSFIVAFFACIFAGLCYAELASTMPVAGSAYTY
                     SYTTLGEVFAWVMGWLLVLEYGVAASTVAAGWSGNVVSLLHNFGMEIPPMLTTSFIQA
                     VPIEGGMTFIQSGGANLLGALGILAVTGLLVLGVSESATVNNIIVAIKVGVLLAFILM
                     GIGFINFENWVPFIPENEGGFKYGLPGIFRAASVIFFAYVGFEAVSTAAAEAKNPQKD
                     IPVGILGSLLVCTLIYMMVAAVLTGVVPFRELGVAAPIALAVDRMGVGWFSILIKVGA
                     VAGLTSVMLILTYGQSRVFYAMAKDGLLPRVFSTLHTKFRTPWIGTIILGMLIAVAAA
                     ALPIEILGDLVSLGTATAFLIVCVSVMHLRRSRPDLVRPFTAPFGVITPLLGIFFALV
                     MIAPLMLDIYAKAMAGDMIPATLLGGYLGLGALIYIFYGFWNSRLAKGLPQHEDSAGP
                     GPMQAQVHGVGDDHA"
     gene            2856..3566
                     /locus_tag="PLO65_09025"
     CDS             2856..3566
                     /locus_tag="PLO65_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD28428.1"
                     /translation="MSSSPIKAFIAPVTPLQQNCTVVWCEKTKKAAVIDPGGEIPRLL
                     AAIAQQGLTVEKIWITHGHLDHAGGTAELAAATGAPIEGPHRDDQFWIDQIPESAARY
                     GIPDAKSFTPDRWLADGDTVQLGEATFEVIHCPGHTPGHVVFFNREARFAQVGDVLFK
                     GSIGRTDFPRGNHQQLIDSITLKLWPLGDDVAFVPGHGPMSTFGFERKANPFVSDIAL
                     GVEAGSLPEMDGVPKRYS"
     gene            3885..4160
                     /locus_tag="PLO65_09030"
     CDS             3885..4160
                     /locus_tag="PLO65_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28429.1"
                     /translation="MRFDSVAGAPDPTITVFQATRVVKETDGLRIESFWGAEGPTPNV
                     VMTLTRNAELLTVKKSALFGDWYYIRCAQVGPLVYRPPQSPGGRRRP"
     gene            4224..>4825
                     /locus_tag="PLO65_09035"
     CDS             4224..>4825
                     /locus_tag="PLO65_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28430.1"
                     /translation="MDKTAHSVVEEPGARILIVDDDPGIRDVIADFLTRHGYRVDQAS
                     DARSMELALQREPADLVVLDVMMPGEDGLSICRRLSGAAGGPGIVMLSAMGEETDRIV
                     GLELGADDYLPKPCNPRELLARVRAVLRRRQEPRQSDGPVGATCEFAGWRLDLVRREL
                     RSPQGVIVNLSSGEFSLLRAFVERPQRVLTRDQLLDLARGP"
     assembly_gap    4826..4995
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4996..>5209
                     /locus_tag="PLO65_09040"
     CDS             <4996..>5209
                     /locus_tag="PLO65_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HRD28431.1"
                     /translation="FVQVLALIIVSQIAAHAVSTAVIFSLPPPPPEIYRLSDVARALR
                     TPGVTAVPDARPLVVRVQARPPTERPM"
BASE COUNT          762 a         1776 c         1690 g          811 t
ORIGIN      
        1 tggtcggcgg cggcgcggga gacgcggaca gtctccagcc gggtcaggcc gctggccgga
       61 tcatagcgcg gcacgcggga caggccggcg tcgatgacca cccgcacgcc ctcgatggtc
      121 aggctggtct cggcgatcga ggtggccagc accaccttgc ggcgacccgg cggcgaggcg
      181 ctgatggcgc gatcctggtc ggcggggtcc atcgcgccgt agagcggggc caggtcgaca
      241 tcggtccggc gcattcgctc ggccagccag tcgcgcgtgc gcagaatctc gccctggcct
      301 ggaaggaaga cgagaatgcc gcccgattcc tcggcgagcg cgcgctcgac ggcccggccg
      361 acctgttcct ccaaccgaag cgcggggtta cggccgacgt ggcgggtctc gacgggaaag
      421 gcgcgaccct ggctctcgat caccggcgcg tcgcccagca gccgcgccac cgcggcgccg
      481 tcgagcgtcg ccgacatcac caggaggcgc aggtcctcgc gcagcagctg ctgcgtatcg
      541 cgcgccaggg ccaggccgag gtcggcgtcg aggctgcgtt cgtggaactc gtcgaacaac
      601 acagccgcga cgccgtcgag gcccggatcg tcctggatca tgcgggtgaa gacaccctcg
      661 gtgaccacct cgatgcgcgt ccgggccgaa accttcgact gcagccgcac ccggaagcca
      721 atggtctccc cgaccgcttc gccaaggctc gacgccatgc gggccgcagc cgcccgagcc
      781 gccagccggc gaggctccag cacgatcagc ttgccgcccg ccgaccacgg ctgatccttg
      841 aggaacaagg ggacgcgcgt ggtcttgccg gcgcccggcg gggccaccag cacggcgcac
      901 gacccatcgg ccagcgcctc gcccaggcgg ggcaggattt cctcaatcgg cagcatcggg
      961 gcgcagtcct agtgcgtcag gagggcctcg tcaccgtaag ccgttgcgcg agacggcttt
     1021 gctgctaacg tccccgacct gttagccgcg ccgacggggg tcgtcggcca ttgggggagc
     1081 gagacgatat gtccgcagat gccgcgccac gcgggaaccg actgtttctc aagaagtcca
     1141 tcgagcaggt ccagcacgag tccaacaccc accagctgaa gcgcaccctc ggggcgtgga
     1201 acctcgtgct gctgggcatc ggctgcatca tcggcgcggg catctatgtg atgaccggca
     1261 acgccgcggc caacttcgcc ggcccggcgg tcatgctgtc gttcatcgtc gccttcttcg
     1321 cctgtatctt cgccggcctc tgctacgccg agctggcctc gaccatgccg gtggccggat
     1381 cagcctacac ctacagctac acgaccctgg gtgaggtgtt cgcctgggtg atgggctggc
     1441 tgctggtgct ggaatacggg gtggcggcgt cgaccgtcgc ggccggctgg tccggcaacg
     1501 tggtcagcct gctgcacaac ttcggcatgg aaatcccgcc gatgctgacg acctcgttca
     1561 tccaggccgt gccgatcgaa ggcggcatga cgttcatcca gagcggcggc gccaacctgc
     1621 tgggcgcgct cggcatcctg gcggtgaccg gtctgctggt gctgggcgtc tcggaatcgg
     1681 cgaccgtcaa caacatcatc gtggcgatca aggtcggcgt gctgctggcc ttcatcctga
     1741 tgggcatcgg cttcatcaac ttcgagaact gggtgccgtt cattccggag aacgagggcg
     1801 gcttcaaata cggcctcccc ggcatcttcc gcgccgcctc ggtgatcttc ttcgcctacg
     1861 tcggattcga ggccgtctcg accgccgcgg ccgaggccaa gaacccgcag aaggacattc
     1921 cggtcggcat cctcggttcg ctgctggtct gcaccctgat ctacatgatg gtcgccgccg
     1981 tactgaccgg cgtcgtgccg ttccgcgaac tgggcgtcgc cgccccgatc gccctggccg
     2041 tcgaccgcat gggcgtgggc tggttctcca tcctgatcaa ggtcggcgcc gtcgccggcc
     2101 tgacctcggt gatgctgatc ctgacctacg gccagagccg ggtgttctac gccatggcca
     2161 aggacggtct gctgccgcgg gtgttctcga cgctgcacac gaagttccgc acgccctgga
     2221 tcggcaccat catcctgggc atgctgatcg ccgtcgccgc cgcggccctg ccgatcgaga
     2281 tcctcggcga ccttgtcagc cttggcaccg ccacggcctt cctgatcgtc tgcgtcagcg
     2341 tcatgcacct gcgccggtcg cggcccgacc tggtgcggcc gttcaccgcg ccgttcggcg
     2401 tcatcacgcc gctgctgggc atcttcttcg ccctggtgat gatcgcgccg ttgatgctcg
     2461 acatctacgc caaggccatg gccggggata tgatccccgc caccctgctg ggcggctatc
     2521 tcggcctggg cgcgctgatc tacatcttct acggcttctg gaactcgcgg ctcgccaagg
     2581 gtctcccgca gcatgaggac agcgctggac ccggtccgat gcaggcccag gttcacggcg
     2641 tcggcgacga ccacgcctga tccagagggg acatgcactt cagtgcgtgc ccccgtcagc
     2701 ttgaagcggc aggggacacg taccgaaggc ggtacatgtc cccggggaaa aatcggaaag
     2761 gcccgggagc gatcccgggc ctttttcgta cccgcccctt tattccggac gcctgaacac
     2821 cgatcatgtg accgtccgcc gtcgcaggag ctttcgtgtc ctcttccccg atcaaggcct
     2881 tcatcgcccc cgtcacgccg ttgcagcaga actgcacggt ggtctggtgc gagaagacga
     2941 agaaggccgc cgtcatcgat ccgggcggcg agatcccgcg gctgctggcc gcaatcgccc
     3001 agcagggtct gaccgtcgag aagatctgga tcactcacgg ccacctcgac cacgccggcg
     3061 gcacggccga gctggccgcc gccaccggcg cgccgatcga gggacctcac cgcgacgacc
     3121 agttctggat cgaccagatt cccgagagcg ccgcgcgcta cggcatcccc gacgcgaaga
     3181 gcttcacccc cgaccgctgg ctggccgacg gcgacaccgt gcaactgggc gaggccacgt
     3241 tcgaggtcat ccactgcccg ggccacaccc ccggtcacgt cgtgttcttc aaccgcgagg
     3301 cgcgcttcgc ccaggtcggc gacgtgctgt tcaagggctc gatcggccgg accgactttc
     3361 cccgcggcaa tcaccagcag ctgatcgact cgatcaccct caagctctgg ccgctgggcg
     3421 acgacgtcgc cttcgtgccc ggccacggcc cgatgagcac cttcggcttc gagcgcaagg
     3481 ccaacccgtt cgtctcggac atcgcgctgg gcgtcgaggc cggcagcctg ccggagatgg
     3541 acggcgtgcc caagcgctac agctgagccg cggcacggcg atccagcccc cattttgggg
     3601 tatacgcccg ttcaccgcgt atgctgtcct tccccccggg ctcacacatg ggaggcatgg
     3661 tgatgagagg gatggcgttg gcggttgccg tttccggcct ggtaacggcc acggcgacta
     3721 cggcggcgga gccgtccgtc accgatctgc tggccggcac ccaatgggtc tggctcggtc
     3781 cgaatcaatc cctggccaag atccggcctg aagtcgtcac ccgagcccga aaccagggct
     3841 gcgacacgcc cacgatgtca ttccggcgga gcggcacaca gctggtgcgg ttcgacagcg
     3901 tcgccggggc tccggacccg accatcacgg tcttccaggc aacgcgggtg gtgaaggaga
     3961 cggacggcct gcggatcgag agcttctggg gggcggaggg gccgacgccc aacgtggtaa
     4021 tgaccctgac ccgcaatgca gagctgctga cggtcaagaa aagcgcgctg ttcggcgact
     4081 ggtactacat ccgctgcgcc caggtcggcc cgttggtcta caggccgcca cagagtcccg
     4141 gcggtcgtcg tcgcccctga tattgcaggg ctgaaatccg gctgccatgt ctgtccgtca
     4201 tagcgcgggg catggagctc gtgatggaca agacagccca cagcgtcgtc gaggagcccg
     4261 gcgcccgcat cctgatcgtc gacgacgacc cgggcatacg ggacgtgatc gccgacttcc
     4321 tgacccgcca tggctaccgc gtcgaccagg cgtccgacgc caggtcgatg gagctggccc
     4381 tgcagcggga gcccgccgac ctggtcgtgc tggacgtgat gatgccgggc gaggacggcc
     4441 tgtcgatctg ccgccgcctg tctggtgcgg cgggcggacc gggcatcgtc atgctgtcgg
     4501 ccatgggcga ggagaccgac cgcatcgtcg gcctcgaact gggggccgac gattacctgc
     4561 ccaagccctg caacccgcgc gaactgctgg cccgggtgcg ggcggtcctg cgccgccggc
     4621 aggagccgcg ccagagcgac gggccggtcg gcgccacctg cgagttcgcc ggatggcggc
     4681 tggacctggt gcgtcgcgag ctgcggtcgc cgcagggcgt gatcgtcaac ctgtcgagcg
     4741 gggaattctc gctgctgcgc gccttcgtcg aacggccgca gcgggtgctg acgcgcgacc
     4801 agctgctgga cctggcccgc ggcccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnngttcg tccaggtcct ggcgctgatc atcgtcagcc agatcgcggc
     5041 ccacgcggtg tcgacggcgg tgatcttcag cctgccgccg ccgccgcccg agatctaccg
     5101 gctgtcggat gtggccaggg ccctgcgcac gccgggcgtc actgccgtgc ccgacgcccg
     5161 gccgctggtg gtgcgggtcc aggccaggcc ccccaccgag cggccgatg
//
LOCUS       DAORNX010000182         5204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11816_length_5204_cov_9.038260, whole genome shotgun sequence.
ACCESSION   DAORNX010000182 DAORNX010000000
VERSION     DAORNX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11816_length_5204_cov_9.038260"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLO65_09045"
     CDS             complement(<1..648)
                     /locus_tag="PLO65_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRD28432.1"
                     /translation="MAILSKRPDVERFLKSPDKAFRAAVIYGRDRGGVRERADRLARA
                     VTAHPDDPFDVALLTEDDVDEGRLANELSAISMMGGRRLVRVRFSTEKIGPDRMVAEA
                     LAGHVEGRFNPDAFFLVEAGAIDRSSPLRKAAEKAAACATIPCYEDEPGDVARLVREA
                     LAADRVGLDAGALEMFVARMPRERGVARQEIERLARLFVERFDVTGIRLTGGEPTV"
     gene            complement(700..1197)
                     /gene="lptE"
                     /locus_tag="PLO65_09050"
     CDS             complement(700..1197)
                     /gene="lptE"
                     /locus_tag="PLO65_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRD28433.1"
                     /translation="MQARLVAIALLLLSPGLAGCGFTPLYAAPGVSSGLSSIESVAPD
                     GRTGFLLRQALDDVLARDPSRPPTWRLTMDLKEVRTPRGRRVDSTASRYELVLIADWK
                     LVSVATGAVVLEGDTRTEATFDRADQPYSAIVANQDAQSRAASELARKIQLQLADWIA
                     RTGGR"
     gene            complement(1202..3775)
                     /gene="leuS"
                     /locus_tag="PLO65_09055"
     CDS             complement(1202..3775)
                     /gene="leuS"
                     /locus_tag="PLO65_09055"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRD28434.1"
                     /translation="MARYNPKDIEPKWRAAWADADVFRVPNDDPRPKYYMLEMFPYPS
                     GNIHMGHVRNYAMGDVVARFRRAQGYAVLHPMGWDAFGMPAENAAMERGIHPKGWTYD
                     NIASMREQLKQLGLSVDWSREFATCDPEYYGKQQAWFLKLLARGLVYRKEATVNWDPV
                     DMTVLANEQVIEGRGWRSGAVVEKKKLTQWFLRITDYADDLIEGLATLDRWPEKVRTM
                     QENWIGRSTGLRMTFDFAGATPAGLADGLEVYTTRPDTLFGASFLGIAPDHPLAESLS
                     AGDPRVAAFIAECRRGGTSEAEIESAEKLGFDTGLRVVHPFIAGETLPVWIANFILMD
                     YGTGAIFGCPAHDQRDLDFARKYGLPVKPVVLPGDAHPATFMVGSEAYTGPGKIFNSG
                     FLDGLTIEQAKAEAVSRIEAMGKGKGATVYRLRDWGVSRQRYWGCPIPVIHCKACGVV
                     PAPADQLPIELPDDVTFDKPGNPLLRHPTWRFTRCPTCGGAAERETDTLDTFVDSSWY
                     FARFTSPHASDPIDKAAADRWLPVDQYIGGIEHAVLHLLYARFVTRALKDEGLVSVAE
                     PFAGLFTQGMLTHETYARQSGEWVDPSQVVLETAGAARRARHAATGETLLIGDIEKMS
                     KSKKNVVAPEEISAQYGIDASRLFILSDSPPDRDVQWTTGGVEGASRFVQRVWSEFDS
                     EAGGGDAPVDPVRSEELRRATHKIIKAVTESIDQFKFNSGIARLYEFLNLLKGFPAAD
                     SSVAAARAEALSALARLIAPYTPHLAEECWLRMGGEGMIVDAPWPEYDPALTVDSELL
                     LPVQVNGKRRGEIRVAADANEAQVRDKALADEAVSVHLAGLTVRKVIVVPGRIVNIVA
                     G"
     gene            complement(3800..4315)
                     /locus_tag="PLO65_09060"
     CDS             complement(3800..4315)
                     /locus_tag="PLO65_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HRD28435.1"
                     /translation="MAFARRPWMRTVSVGVLALGLVGVSACGSTGGARTFQTQEEEGF
                     HPFGLGRGGPPKGGAGAPAQLGVNGYLWRATLDTLSFMPLASADPYGGVVITDWYVNP
                     EKPDERFKATVYILDTRLRADGLNVTVFKQIGDGAGGWTDAPTSAQTETDIENAILTR
                     ARQLRLANISG"
     gene            4602..5015
                     /locus_tag="PLO65_09065"
     CDS             4602..5015
                     /locus_tag="PLO65_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28436.1"
                     /translation="MAKVRSIAVMAALALTGAATTALAQSAPARPRVQPAPDAFVVRG
                     DFNLRDAQFGPQTGRRTLVWDAKKGRWGLMLDISPRMGADAQARDLEAGAFFRVTPQL
                     RVGGAVGVGSQVTPERKPGAGEEVPRVRLETAFKF"
     gene            complement(5012..>5204)
                     /locus_tag="PLO65_09070"
     CDS             complement(5012..>5204)
                     /locus_tag="PLO65_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRD28437.1"
                     /translation="VSPVFASRSPSAGRPMGVEAFAALVRAAPLPVYALGGVNARTAA
                     RLTESGAQGLAMVEGLAGA"
BASE COUNT          797 a         1786 c         1812 g          809 t
ORIGIN      
        1 caccgtgggc tcgccgccgg tgagccggat gccggtgacg tcgaaccgtt cgacgaacag
       61 gcgggccagg cgctcgatct cctgccgggc tacgccgcgt tcgcggggca tgcgggcgac
      121 gaacatctcc agggcgccgg cgtcgaggcc gacccggtcc gccgccaggg cctcgcggac
      181 cagccgggcc acatcgccgg gctcgtcctc gtaacagggg atggtggcgc aggccgcggc
      241 cttctcggcc gccttgcgca gcggcgagct gcggtcgatc gcgccggcct cgacgaggaa
      301 gaaggcgtcg ggattgaagc gaccctcgac atgacccgcc agggcttccg cgaccatacg
      361 gtccggtccg atcttctccg tcgagaagcg cacccggacc agccgccggc cacccatcat
      421 cgagatggcg ctgagctcgt tggccagccg cccctcgtcg acatcgtcct cggtcagcag
      481 ggcgacatcg aacggatcgt ccggatgggc ggtgaccgcc cgggccagcc ggtcggcccg
      541 ctctcgcacc ccgccgcggt cgcggccata gatgacggcg gcgcggaagg ccttgtccgg
      601 cgacttcagg aagcgttcga catcgggccg tttcgagagg atggccatgt cacgccttct
      661 ggcggagctg ggggcggacc tgccgcacgg gccgcgggcc taccggccgc cggttcgggc
      721 gatccagtcc gccagctgca gctggatctt gcgggccagc tccgaggcgg cgcggctctg
      781 ggcgtcctgg ttggcgacga tggccgaata gggctggtcc gcccggtcga aggtcgcctc
      841 ggtgcgggta tcgccctcca ggaccaccgc gccggtggcg acgctgacca gcttccagtc
      901 ggcgatcagc acaagctcgt agcgactggc cgtggagtcg acccgccgcc cgcggggggt
      961 gcggacttcc ttcaggtcca tggtcaaccg ccaggtcggc ggcctggacg ggtcgcgcgc
     1021 cagcacgtcg tcgagggcct ggcgcagcag gaaacccgtc cggccatcag gcgccacgct
     1081 ctcgatcgac gacaggccag acgaaacgcc gggcgcggcg tagagcggcg tgaagccgca
     1141 accggcgagg ccgggcgaga gcaacagcag ggcgatggcg acgaggcggg cctgcatcgc
     1201 cctatcccgc cacgatgttg acgatacggc cgggcaccac gatcaccttt cgcacggtca
     1261 ggcccgcaag gtggaccgac acggcctcgt ccgccagagc cttgtcgcgc acctgcgcct
     1321 cgttcgcgtc agccgccacg cggatctcgc cgcggcgctt gccattgacc tggaccggca
     1381 gcagcagttc ggaatcgacg gtcagggctg gatcgtactc gggccagggg gcgtcgacga
     1441 tcatcccctc gccgcccatc cgcagccagc attcctcggc caggtgcggg gtgtagggtg
     1501 cgatgagccg ggccagcgcc gagagcgctt cggccctggc cgccgctacg ctgctgtcgg
     1561 cggcgggaaa gcccttgaga aggttcagga attcatacag ccgggcgatg ccgctgttga
     1621 acttgaactg gtcgatcgat tctgtgaccg ccttgatgat cttgtgcgtc gcacggcgca
     1681 gctcctcgga gcgcaccggg tcgaccggcg cgtcgccgcc gccggcctcg ctgtcgaact
     1741 ccgaccagac ccgctggacg aagcggctgg cgccctcgac gccgccggtg gtccattgca
     1801 cgtcgcgatc gggcgggctg tcggacagga tgaacagccg agaggcgtcg atgccgtact
     1861 gggcggagat ctcctccggc gccacgacgt ttttcttcga cttcgacatc ttctcgatgt
     1921 cgccgatcag cagggtttcc ccggttgcgg cgtgccgggc ccggcgcgcc gcgcccgccg
     1981 tctccagcac cacctgcgag gggtcgaccc attcgccgct ctgtcgcgca taggtttcgt
     2041 gggtcagcat gccctgggtg aacaggcccg cgaacggctc ggcgaccgaa accagcccct
     2101 cgtccttcag ggcgcgggtg acgaaccggg cgtacagcag gtgcaggacg gcgtgctcga
     2161 tgccgccgat gtactggtcc acgggcagcc aacggtccgc cgcagccttg tcgatcgggt
     2221 cgctggcgtg cgggctggtg aagcgggcga agtaccagct cgaatccacg aaggtgtcga
     2281 gcgtgtcggt ttcacgctcg gcggcgcccc cgcaggtcgg acagcgggtg aagcgccagg
     2341 tcgggtgccg cagcagcgga ttgcccggct tgtcgaaggt cacgtcatcc ggcaactcga
     2401 tcggcaactg atcggccggc gcggggacca cgccacaagc cttgcagtgg atcaccggga
     2461 tcggacagcc ccagtagcgc tggcgcgaga cgccccagtc gcgcagacgg taaaccgtcg
     2521 cgcccttgcc cttgcccatg gcctcgatgc gggacaccgc ctcggccttg gcctgttcga
     2581 tggtcaggcc gtcgagaaac ccggaattga agatctttcc ggggccggtg taggcctccg
     2641 acccgaccat gaaggtggcc ggatgggcgt cgccgggcag caccaccggc ttgaccggca
     2701 ggccatactt gcgggcgaaa tccaggtccc gctggtcgtg ggccgggcag ccgaagatgg
     2761 cgcccgtgcc gtagtccatc aggatgaagt tggcgatcca caccggcagg gtctcgccgg
     2821 cgatgaacgg atggaccacc ctcaggcccg tgtcgaagcc cagcttttct gcgctctcga
     2881 tctccgcctc ggaggtcccg ccgcggcggc attcggcgat gaacgcggcc actctggggt
     2941 cgccggcgga cagggattcg gcaagcggat ggtcgggggc gatgcccaga aagcttgccc
     3001 cgaacagggt gtcggggcgg gtcgtgtaga cttccagccc gtccgccagg ccagccggcg
     3061 tggcgccggc gaagtcgaag gtcatgcgca gaccggtcga ccgaccgatc cagttttcct
     3121 gcatggtccg gaccttttcc ggccagcggt cgagggtcgc cagaccctcg atcaggtcat
     3181 cggcgtagtc cgtgatccgc aggaaccact gggtgagctt cttcttctcg accaccgcgc
     3241 ccgaacgcca gccgcggccc tcgatgacct gctcgttggc cagcacggtc atgtccaccg
     3301 ggtcccagtt gaccgtggcc tccttgcggt agaccaggcc gcgcgccagc agcttcagga
     3361 accaggcctg ctgcttgcca tagtattccg ggtcgcaggt ggcgaactcg cggctccagt
     3421 cgaccgacag acccagctgc ttcagctgct cgcgcatcga ggcgatgttg tcgtaggtcc
     3481 agcccttggg gtggatgccg cgctccatcg ccgcgttctc ggccggcatg ccgaaggcgt
     3541 cccagcccat cgggtgcagc accgcataac cctgggccct gcggaatcgg gccacgacat
     3601 cgcccatggc gtagttgcgc acatggccca tgtggatgtt tcccgaagga taggggaaca
     3661 tctccagcat gtagtacttc gggcgcggat cgtcgttcgg gacacggaaa acatccgcgt
     3721 ccgcccaggc ggcgcgccac ttgggctcaa tgtccttcgg attgtatctg gccattcggc
     3781 gagggtgcga tgtccgggat cagccggaga tgttggccag gcgcagctgg cgcgcccggg
     3841 tgaggatggc gttctcgatg tcggtctcgg tctgcgccga ggtcggcgca tcggtccagc
     3901 cgcccgcgcc gtcgccgatc tgcttgaaca ccgtgacatt caggccgtcg gccctaagtc
     3961 tcgtgtcgag aatgtagacg gtcgccttga agcgttcgtc gggcttttcc ggattgacgt
     4021 accagtcggt gatcacgacg ccgccgtacg ggtccgccga ggccagcggc atgaacgaca
     4081 gggtgtccag cgtggcccgc cacaggtagc cgttgacgcc cagctgggcc ggcgcgccgg
     4141 cgccgccctt gggaggaccg ccgcggccga gtccgaaagg atggaaacct tcttcttcct
     4201 gggtctggaa agtccgggcg ccgcccgtcg agccgcaggc ggacacgccc acgagaccca
     4261 gggccagaac cccgacggag acggtccgca tccacggcct gcgcgcaaac gccatattct
     4321 ctcgctccac tcgttccggc cgtcgcttcc tcctggaaag cgtatcggcg ttcacccccg
     4381 tgcccatgaa gtgcgtctac aacatggcct cgtcgccgcc aaacgggaat cgcgtgtctc
     4441 tcgcgccaca ggcgaggcag gaatcggcgg agtcggccca ggactcaccc cagggtctgt
     4501 tgaccccacc gcaagagggc ctttaatcgc gatcaaggtc cacccatctt caatactgaa
     4561 gctggattgg acagatcggc tgggggagcc ggaacggcgg aatggcgaag gttcggagca
     4621 tcgcggtgat ggcggcgctg gccctgacag gggcggcgac gacggcgctt gcgcagagtg
     4681 ctccggcccg gccgcgggtg caaccagcgc ccgacgcctt cgttgtccgc ggcgatttca
     4741 atctgaggga cgcccagttc gggccccaga cgggtcgccg cacgctggtg tgggacgcca
     4801 agaagggccg gtggggcctg atgctcgaca tctcgccgcg catgggcgcc gacgcccagg
     4861 cccgcgacct cgaggccgga gccttcttcc gggtcacgcc gcagttgcgg gtcggcggcg
     4921 cggttggcgt cgggtcccag gtgacgccgg agcgcaagcc cggcgccggc gaagaggtgc
     4981 cccgcgtgcg ccttgagaca gccttcaagt tctaggcgcc ggccagacct tccaccatgg
     5041 ccagtccttg cgcccccgac tccgtcagcc gggcggccgt tcgcgcattg acgccaccca
     5101 gggcgtagac ggggagcggc gcggcgcgca ccagggcggc gaaggcctcc acccccatgg
     5161 ggcgaccggc tgacggactg cggctggcga acaccggcga gacc
//
LOCUS       DAORNX010000183         5203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11820_length_5203_cov_7.570124, whole genome shotgun sequence.
ACCESSION   DAORNX010000183 DAORNX010000000
VERSION     DAORNX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11820_length_5203_cov_7.570124"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLO65_09075"
     CDS             <1..250
                     /locus_tag="PLO65_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28438.1"
                     /translation="EPDALVRVLNVVTLLQARMRRLEAEPAEGGLSIVLEVDDHGDHR
                     AEALARRLEACPAVKRLTFGWRGQPPEQGQVILTQGTG"
     gene            274..807
                     /locus_tag="PLO65_09080"
     CDS             274..807
                     /locus_tag="PLO65_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRD28439.1"
                     /translation="MTKPAVERFLERGLFASRWLMAPMYVGLVIALLALIVIFAQELI
                     HAVPPILSGSAHAEDAILLALSLIDLSLAGNLVLIVIFSGYENFVSKLDIGETEDRPS
                     WMGTVDFSGLKMKLVASIVAISAIALLKAFLEASEGAGSIDATRMMWLVVIHLTFVVS
                     GVLLALMDWLASLTDKH"
     gene            complement(810..1391)
                     /locus_tag="PLO65_09085"
     CDS             complement(810..1391)
                     /locus_tag="PLO65_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HRD28440.1"
                     /translation="MDRRTLILSGLAAGVSGAASAALAQTTDPADGPRTYTAEEIINA
                     TSDFLGVTAEAAGAAIERIFKQNGRPTAYIAGEEGSAAITLGARYGRGLLYMKEQPTQ
                     EVYWQGPSIGFDVGGNASRVFTLCYNLHYPDMIYRRFPGVEGSAYMVAGLGVNYQRAD
                     GITLAPIRAGVGLRLGANVGYLAYSRKRNILPF"
     gene            complement(1475..4576)
                     /locus_tag="PLO65_09090"
     CDS             complement(1475..4576)
                     /locus_tag="PLO65_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD28441.1"
                     /translation="MRNISSWSIRNPIPVIMLFIMLTLAGLIGFNAMRINNNPDVDFP
                     LIQVVAARPGAAPSEMETQVTRLIEDSISGLSGVRHTRSTVTDGVSVTMIEFELSTDT
                     EKATNDVRNAMGILRADLPQDMQEPTVKLWDITGDPLITYVVRSGSMSPEQLSWFVDN
                     TVSRRLLAVSGVGQVNRLGGVEREIRIDLDPDRLASFGVTAAQVSQALVNVNNDLPGG
                     RVTVGGAERSIRTLGAAGSVQELADTRVPVSGGSVRLGDLGRVEDNWTEPRSRARYDG
                     QEVVTFNFLRSRDASEVKTAERVRKEIAAIDAAHPELTIEQINANVEFAEESYIASLE
                     AVGLGALLAVIVVWLFLRDWRATLIAAVAMPLSLIPTFAVLEPLGQSLNMITLLALSL
                     TIGILVDDAIVEIENIVRHMRGGKAPYPAAMEAADEIGLAVVATTLTIVAVFAPVGFM
                     PGVIGQFFKAFALAACVSVLFSLVVARMLTPLMGAYLLKHKPHEDADPPWMSWYLGRL
                     KWALANRWKVVAMGGVVFVATGALTPLVPVEVQPSVDFGRAAFSVELPPGSTLDQTDD
                     VVARVTRELRRQPEVTHVYAQVGGGNVNAATVTAELVDKGRRDVTQQEFTRKMVDAFK
                     IVPGARIGVPTQLGGGPTDGSVYSYAIQSDDGETLSAAARRIEREMRSVPGLANVINT
                     AAIARPEILVTPRADEAARLGVSAGALSQAIRVATIGDVDQLLPKYNLGDRQVPIRLM
                     LSENARGDITVLENLRVPTASGGSVPLNAVADVTYNAGPSQVDRLDRSRVATITAELN
                     GITSGQAALAVNALPSVKNLPEGVKQGMTGDIEFISEMIIGFAGAFITGLVLMYAVLA
                     LLFKSFTHPITIMAALPLAIGGAFVGLLVMGSSFSISTLIGILMLMGIAAKNSILLVE
                     YALEAIKNGMGRYEALMDAAHKRARPILMTTVAMGAGMAPTALGWGADVEFRQPMAVA
                     VVGGLITSTLLSLLYIPAIFTIIDDMRGWGHRRLDRMFASQKVLKPTTQARARPATPE
                     QQG"
     gene            complement(4586..>5203)
                     /locus_tag="PLO65_09095"
     CDS             complement(4586..>5203)
                     /locus_tag="PLO65_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD28442.1"
                     /translation="AQVSAAEAGLSEIRTRLGQAVVRAPVSGLVASRKVVQGQIVSPG
                     TELFRIVRDGRLELNAEVPETQLAQVRAGMPATVSSDQLGQVPGRVRIVTPQVDSRSR
                     VGVARIALSAAGGFRPGMFAKGEINVGEQPAVTAPSIAVVYRDNKPGVFVVDAQSAVR
                     FRPIVVGARTKDSVEAASGLAAGEKIVVQGAGFLADGDRVRVVSK"
BASE COUNT          785 a         1753 c         1846 g          819 t
ORIGIN      
        1 cgagcccgac gctctggtgc gggtgctcaa cgtggtcacc ctgcttcagg cgcggatgcg
       61 gcggctggag gcggaaccgg ccgagggcgg gctgtcgatc gtgctcgagg tcgacgatca
      121 tggcgatcac agggccgagg cgctggcgcg gcggctggag gcctgcccgg cggtgaaacg
      181 gctgacgttc ggctggcgcg gccagccccc ggaacaggga caggttatcc tgacccaagg
      241 gacaggataa cctgtctcca aagagggatt cgcatgacca agcctgccgt cgaacggttt
      301 ctggagcgcg gcctgttcgc ctcgcgctgg ctgatggcgc cgatgtatgt cgggctggtc
      361 atcgccctgc tggcgttgat cgtgatcttc gcccaggagc tgatccacgc cgtgccgccg
      421 atcctgtccg ggtcggccca cgccgaggac gccatcctgc tggccctgtc gctgatcgac
      481 ctgtcgctgg ccggcaacct ggtgctgatc gtgatcttct cgggctacga gaacttcgtc
      541 tccaagctcg acatcggcga gacggaggac cgcccgtcat ggatgggcac ggtcgacttt
      601 tccggcctga agatgaagct ggtggcctcg atcgtggcta tctcggccat cgcgctgctg
      661 aaggcctttc tcgaggccag cgaaggcgcc gggtcgatcg acgcgacccg gatgatgtgg
      721 ctggtggtca tccacctgac cttcgtggtc tccggcgtgc tgctggccct gatggactgg
      781 ctggccagcc tgaccgacaa gcactgaggt cagaacggca ggatgttgcg cttgcggctg
      841 taggccaggt agccgacgtt cgcgccgagg cgcaggccga cgccggcgcg gatcggggcc
      901 agggtgatgc cgtcggcccg ctggtagttc acgccgagac ccgcgaccat gtaggccgag
      961 ccctcgaccc ccgggaaccg gcggtagatc atgtccgggt aatgcaggtt gtagcagagg
     1021 gtgaagaccc ggctggcgtt gccgccgacg tcgaagccga tcgacgggcc ctgccagtag
     1081 acctcctgcg tcggctgctc cttcatgtac agcagcccgc ggccgtagcg ggcgcccagg
     1141 gtgatggcgg ccgagccttc ctcgccggcg atgtaggccg tcggacggcc gttctgcttg
     1201 aagatccgct cgatcgccgc gccggcggcc tcggccgtca caccgaggaa gtccgaggtg
     1261 gcgttgatga tctcctcggc ggtgtaggtc cgcggaccgt cggccggatc ggtggtctgc
     1321 gccagggcgg ccgaggcggc gccgctgacg cccgcggcga ggccggagag gatgagggtg
     1381 cggcggtcca ttgcggtcgc tccgagtcgg ctggaaacgg ggtcagactc ccactccaat
     1441 acgaccggtt tgcggcgcga aggttatcgc gaggttaacc ctgttgctcc ggcgtcgccg
     1501 gccgcgcccg cgcttgcgtc gtcggtttga gcaccttctg gctggcgaac atccggtcca
     1561 gccggcgatg gccccagccc cgcatgtcgt cgatgatcgt gaagatcgcc gggatgtaga
     1621 gcagcgacag cagggtcgag gtgatcaggc cgccgacgac ggccacggcc atcggctgac
     1681 ggaactccac gtcggccccc cagccgaggg ccgtgggcgc catgcccgcg cccatcgcca
     1741 cggtggtcat caggatcggc cgggcccgct tgtgggcggc gtccatcagg gcctcgtagc
     1801 ggcccatgcc gttcttgatc gcctcgaggg cgtactccac cagcaggatc gagttcttcg
     1861 ccgcaatgcc catcagcatc aggatgccga tcagcgtcga gatcgagaag ctgctgccca
     1921 tgaccagcag cccgacgaac gccccgccga tggccagcgg caaggcggcc atgatggtga
     1981 tcggatgggt gaagcttttg aacagcaggg ccagcacggc gtacatcagc accaggccgg
     2041 tgatgaaggc gccggcgaag ccgatgatca tctcgctgat gaactcgatg tcgcccgtca
     2101 tgccctgctt gacgccttcc ggcaggttct tgaccgaggg cagcgcgttg acggccagcg
     2161 cggcctggcc gctcgtgatg ccgttgagtt cggcggtgat ggtcgcgacc cgcgaccggt
     2221 cgaggcggtc gacctgcgag gggccggcgt tgtaggtcac gtcggcgacc gcgttcagcg
     2281 ggaccgaacc gccgctggcg gtcggcaccc gcaggttctc gagcaccgtg atgtctccgc
     2341 gcgcgttttc gctcagcatc aaccggatcg gcacctggcg gtcgccgaga ttgtacttgg
     2401 gcagaagctg gtcgacatcg ccgatggtgg ccacgcggat cgcctgcgac agcgcgccgg
     2461 ccgagacgcc caggcgagcg gcctcgtcgg ctcgcggcgt gaccaggatc tcgggccgcg
     2521 cgatcgcggc cgtgttgatc acgttggcca gtccgggaac cgagcgcatc tcgcgttcga
     2581 tcctgcgggc cgcggcggac agcgtctcgc cgtcgtcgct ctggatggcg taggaataga
     2641 cgctgccgtc ggtcggaccg ccgccgagct gggtcggcac cccgatccgc gcgcccggca
     2701 cgatcttgaa ggcgtcgacc atcttgcgcg tgaattcctg ctgggtgacg tcgcgccggc
     2761 ccttgtccac cagttcagcg gtcacggtgg cggcgttgac gttgcctccc cccacctggg
     2821 cgtagacgtg ggtcacctcc ggctgcctgc ggagctcgcg ggtcacgcgg gcgacgacgt
     2881 catcggtctg gtccagggtc gaacccggcg gcaactcgac cgagaaggcg gcgcggccga
     2941 agtcgaccga cggctgcacc tccaccggca ccagcggcgt cagcgcgccg gtggccacga
     3001 acaccacgcc gcccatggcg acgaccttcc agcggttggc cagcgcccac ttcagtcgcc
     3061 ccagatacca gctcatccac ggcggatcgg catcctcgtg cggcttgtgc ttgagcagat
     3121 aggcgcccat cagcggcgtc agcatccggg cgaccaccag cgagaacagc accgagacgc
     3181 aggccgccag ggcaaaggcc ttgaagaact ggccgatgac gcccggcatg aagccgaccg
     3241 gcgcgaacac cgcgacgatg gtcagggtgg tggccacgac ggccaggccg atctcgtcgg
     3301 ccgcctccat ggccgccggg tacggagcct tgccgccacg catgtggcgg acgatgttct
     3361 cgatctccac gatggcgtcg tcgaccagga tgccgatggt cagcgacagc gccagcagcg
     3421 tgatcatgtt gagcgactgg cccaggggct cgagcaccgc gaacgtcggg atcagcgaca
     3481 gcggcatggc caccgccgcg atcagggtcg cgcgccagtc acgcaggaac agccacacca
     3541 cgatgaccgc cagcagcgcg cccaggccca ccgcttcgag cgaggcgatg tagctttcct
     3601 cggcgaactc cacgttggcg ttgatctgct cgatggtcag ctcggggtgg gccgcgtcga
     3661 tcgccgcgat ctccttgcgg acccgctcgg cggtcttcac ctcgctggcg tcgcgcgagc
     3721 gcaggaagtt gaaggtcacc acctcctggc catcgtagcg tgcgcgcgac cggggctcgg
     3781 tccagttgtc ctcgacccgg ccgaggtcgc ccagtcggac gctgccgccg gagacgggca
     3841 cgcgggtgtc ggccagctcc tgcaccgacc cggcggcgcc cagggtgcgg atcgagcgct
     3901 cggcgccgcc gacggtcacg cgaccgccgg ggaggtcgtt gttcacattg accagcgcct
     3961 ggctgacctg cgccgccgtg acgccgaacg aggcaagacg gtcgggatcg aggtcgatgc
     4021 ggatctcgcg ctcgacgccg cccagacggt tgacctgacc cacgccggac acggccagca
     4081 gccgtcggct gacggtgttg tcgacgaacc acgacagctg ttcggggctc atcgagccgg
     4141 agcggacgac gtaggtgatc agcggatcgc cggtgatgtc ccacagcttg acggtcggct
     4201 cctgcatgtc ctgcggcagg tcggcgcgca ggatgcccat ggcgttgcgg acgtcgttgg
     4261 tggccttctc ggtgtcggtc gagagctcga actcgatcat cgtcacggac acgccgtccg
     4321 tgaccgtcga ccgcgtgtgc cgcaccccgc tgaggccgga aatcgagtcc tcgatcagcc
     4381 gcgtgacctg ggtctccatc tcgctggggg cggcgccggg gcgggcggcg acgacctgga
     4441 tcaacgggaa gtcgacgtcg gggttgttgt tgatccgcat ggcgttgaag ccgatcaggc
     4501 cggccagggt cagcatgatg aacagcatga tgaccgggat cggattccgg atcgaccagg
     4561 aggagatgtt gcgcatggcc cgcgcctact tgctgacgac gcggacgcgg tcgccgtcgg
     4621 cgaggaaacc cgcgccctgc acgacgatct tctcgcccgc ggccaggccg gaggctgcct
     4681 cgacgctgtc cttcgtcctg gccccgacca cgatgggtcg gaaccggacc gccgactggg
     4741 cgtcgaccac gaagacgccc ggcttgttgt cgcggtagac gaccgcgatc gagggcgccg
     4801 tcaccgccgg ctgttcgccg acgttgatct cgcccttggc gaacatcccg ggccggaagc
     4861 cgccggcggc ggacagcgcg atgcgggcga cgcccacgcg gctgcggctg tcgacctggg
     4921 gcgtcacgat gcgcaccctt cccgggacct ggccgagctg gtcggagctg acggtggccg
     4981 gcatgccggc gcggacctgg gccagctggg tctccggcac ctcggcgttg agttcaagcc
     5041 ggccgtcgcg gacgatccgg aacagctcgg tcccgggcga gacgatctgg ccctgcacca
     5101 ccttgcgcga ggcgaccagg cccgagaccg gcgcccggac cacggcctgg cccagccggg
     5161 tgcggatttc cgacagcccg gcctcggcgg ccgagacctg ggc
//
LOCUS       DAORNX010000184         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11826_length_5202_cov_9.522829, whole genome shotgun sequence.
ACCESSION   DAORNX010000184 DAORNX010000000
VERSION     DAORNX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_5202_cov_9.522829"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1664)
                     /locus_tag="PLO65_09100"
     CDS             complement(180..1664)
                     /locus_tag="PLO65_09100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRD28443.1"
                     /translation="MSGILSLVTFLPLAGVAAILALRFLGQPGEARDTAGKWIALATT
                     LATFALSVKLTLDFDPANAGFQFLEEIPWFAGLDYRMGVDGISVLFVLLTAFLLPLCI
                     IASWKTIEKRVLEYMIAFLVLETLVIGVFCALDLIVFYLFFEAGLVPMFLIIGIWGGK
                     NRVYAAFKFFLYTLLGSVLMLAAILAMIGISGTSSIPELMTYRFDPAIQTWLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFMRFSLPMFPSASELFT
                     PLVFGLSVIAIIYTSLVAFRQTDIKKLIAYSSVAHMGFVTMGIFSGNVQGEQGAIFQM
                     LSHGVISGALFLCVGVVYDRMHTREIAFYGGLVNRMPIYAAVFMLFTMGNVGLPGTSG
                     FVGEILTMAGAYGASTWTALFAATGVILSAVYALSLYRRVVFGEITNPALAQISDLTW
                     RELVVFAPLILSTLYLGVQPDVVFNITGPSVDALVGAWRAAIAG"
     gene            complement(1668..3710)
                     /gene="nuoL"
                     /locus_tag="PLO65_09105"
     CDS             complement(1668..3710)
                     /gene="nuoL"
                     /locus_tag="PLO65_09105"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRD28444.1"
                     /translation="MQQLVTVLLFAPLLGALVAGLFGRRIGALASQSVTTGLLLLACG
                     LSWYTFSQWTWGGLEPFTVSLAPFINVGDFQSAWSIRIDGLSAVMLVVVTTVSALVHL
                     YSWGYMAEDESRPRFFAYLSLFTFAMLALVTAADFMQLFFGWEGVGLASYLLIGFWFK
                     KPTANAASIKAFVVNRVGDFGFALGIMTVFWAYGTIQFAELFPMIADSASKTWTFAGQ
                     SWPLVDLACVLLFIGAMGKSAQFFLHTWLPDAMEGPTPVSALIHAATMVTAGVYMVCL
                     LSPMFEYAPMARNVVTLIGAITALFAATVGLAQNDIKRVIAYSTCSQLGYMFFAAGVG
                     AYQAAMFHLFTHAFFKALLFLGAGSVIHGMHHEQDMRKMGGLWKHLPVTYAVMTIGTL
                     AITGFGIPGLDLGFAGFYSKDTIIEASWAAGRENGFAWFAFIISLLAAGLTAFYSWRL
                     AFMTFHGTAKWGEQAAHGHDDHHAHAAHDDHAHDDHGHGHSPHESPLSMLVPLLVLAI
                     GALAAGYVFVENFVGEAQVEFWRGAIFNGPHNHVLHAMHEAPPWVIWAPLVVSAIGFA
                     GAWWVYIAREGMGARIAARRGPVWSFLYNKWFFDELYDATFVRLARFLGDLFWKGGDQ
                     KLIDGLGPDGVSAVSLAVGKRTGRLQSGYVYHYAFVMLLGVAGLLSFALYAFSRGS"
     gene            complement(3714..4019)
                     /gene="nuoK"
                     /locus_tag="PLO65_09110"
     CDS             complement(3714..4019)
                     /gene="nuoK"
                     /locus_tag="PLO65_09110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRD28445.1"
                     /translation="MIGLTNYLTVAAILFTIGVFGIFVNRKNVIIILMSIELILLAVN
                     INLVAFSVFLHDVVGQIFAMFVLTVAAAEAAVGLAILVTFFRNRGDIAVDDASVMKG"
     gene            complement(4016..4636)
                     /locus_tag="PLO65_09115"
     CDS             complement(4016..4636)
                     /locus_tag="PLO65_09115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRD28446.1"
                     /translation="MALQAIAFYIMAATTISAALAVVFARNPVHSVLFLIAAFFSAAG
                     LFVLMGAEFLAMLLVVVYVGAVAVLFLFVVMMLDVDFTQLREGFSRYLPVGGLVAGIL
                     TVEMIIVATTVASQGAASASPGPDASAGTDVTNAETIGRVLYTDYVYFFQAAGLVLLV
                     AMIGAIVLTLRHKPGVKRQNIAAQNARTPATGMTIVDIKPGEGISE"
     gene            complement(4720..>5202)
                     /gene="nuoI"
                     /locus_tag="PLO65_09120"
     CDS             complement(4720..>5202)
                     /gene="nuoI"
                     /locus_tag="PLO65_09120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRD28447.1"
                     /translation="RIGQAIKGAALLDFVGAFGLAMKYMIAPKKTVIYPNERGPQSPR
                     FRGEHALRRYPSGEERCIACKLCEAVCPAQAIYIEAEPRADGSRRTTRYDIDMVKCIY
                     CGLCQEACPVDAIVEGPNTEFATETREELYYDKERLLDNGDRWEREIAKNLELDAAYR
                     "
BASE COUNT         1034 a         1668 c         1768 g          732 t
ORIGIN      
        1 ggcccggctc gccggtcacg gccgcgacgc cggcggccgc cagcaccagc atggccgcca
       61 ggtcgaacac cggtccgccc ttgccgcgga aggccccgac catcagcagc agcaagccgc
      121 cgaccgcgag gatcagttca ggccggacga ggttcagatc ggcgagggtc atcggcggct
      181 cacccggcga tcgcggcgcg ccaggcgccg accagggcgt caaccgacgg cccggtgatg
      241 ttgaagacga cgtcgggctg cacgcccagg tacagggtcg acaggatcag cggcgcgaac
      301 accaccagct cgcgccaggt caggtcgctg atctgggcca gggccggatt ggtgatctcg
      361 ccgaacacca cccgccgata gaggctcagg gcgtagacgg ccgacaggat tacgcccgtg
      421 gccgcgaaca gcgccgtcca ggtcgaggcg ccgtaggccc cggccatggt caggatctcg
      481 ccgacgaagc cgctggtgcc cggcaggccg acgttgccca tggtgaacag catgaatacc
      541 gcggcataga tcggcatgcg gttcacaagg ccgccgtaga acgcgatctc gcgagtgtgc
      601 atgcggtcgt agaccacgcc gacacagagg aacagcgcac cggagatgac cccgtggctg
      661 agcatctgga agatggcccc ctgctcgccc tgcacgttgc cgctgaagat gcccatggtc
      721 acgaagccca tgtgggcgac cgacgaatag gcgatcagct tcttgatgtc ggtctgccgg
      781 aaggcgacca gcgaggtata tatgatggcg atcaccgaca ggccgaacac cagcggggtg
      841 aacagctcgc tggcgctggg gaacatcggc aggctgaagc gcatgaagcc gtaaccgccc
      901 atcttcagca ggatgccggc gaggatcacg gagcccgccg tgggcgcctc gacgtgggcg
      961 tcaggcagcc aggtgtgcac cggccacatc ggcatcttga ccgcgaagct ggcgaagaag
     1021 gccagccaca gccaggtctg gatggccggg tcgaagcggt aagtcatcag ttcagggatc
     1081 gagctggtgc ccgaaatgcc gatcatggcg aggatcgccg ccagcatcag gaccgaaccc
     1141 agcagcgtgt agaggaagaa cttgaacgcc gcgtagaccc ggttcttgcc gccccagatg
     1201 ccgatgatca ggaacatcgg caccaggccc gcttcgaaga acaggtagaa gacgatcagg
     1261 tccagcgcgc agaacacccc gatgaccagg gtctcgagga ccaggaaggc gatcatgtac
     1321 tcgaggacgc gcttctcgat cgtcttccag ctggcgatga tgcacagcgg cagcaggaag
     1381 gccgtcagca gcacgaacag caccgagatg ccgtccacgc ccatgcggta gtcgagaccc
     1441 gcgaaccacg gaatctcctc caggaactgg aagccggcgt tggccgggtc gaagtcgagc
     1501 gtcagtttca ccgacagggc gaaggtcgcc agagtggtcg ccagggcgat ccactttccg
     1561 gccgtgtcac gggcctcgcc cggctggccg aggaagcgca gcgccaggat ggcggcgacg
     1621 ccggccagcg gaaggaaggt gacaagggag aggatgccgc tcatcgatca gctcccccgc
     1681 gagaaggcgt agagcgcgaa ggacaacaga ccggcgaccc ccagcagcat gacaaaggcg
     1741 tagtggtaga cgtagcccga ctgcagccgg ccggtgcgct tgccgaccgc cagcgagacc
     1801 gccgaaacgc cgtccgggcc gaggccatcg atcagcttct ggtcaccgcc cttccagaac
     1861 aggtcgccca ggaaacgggc cagccgcacg aaggtcgcgt cgtagagttc gtcgaagaac
     1921 cacttgttgt agaggaagct ccagaccggg ccgcgacggg cggcgatccg agcgcccatg
     1981 ccctcgcggg cgatatagac ccaccaggct ccggcgaagc cgatcgccga gaccacgagc
     2041 ggcgcccaga tgacccacgg cggcgcctcg tgcatggcgt gcagcacgtg gttgtgagga
     2101 ccgttgaaga tcgcaccgcg ccagaactcg acctgcgcct cgccgacgaa gttctcgacg
     2161 aagacgtagc cggccgccag ggcgccgatc gccagcacca gcagcggaac cagcatcgac
     2221 agggggctct cgtgcgggct gtggccatgg ccatggtcgt cgtgagcatg gtcatcatgc
     2281 gccgcgtggg catggtgatc gtcgtggccg tgggccgcct gctcgcccca cttggccgtg
     2341 ccatggaagg tcatgaaggc caggcgccag ctgtagaagg cggtcaggcc ggcggccagc
     2401 aggctgatga tgaaggcgaa ccaggcgaag ccgttctcgc ggccggcggc ccacgacgcc
     2461 tcgatgatgg tgtccttgga gtagaagccc gcgaagccca ggtcgaggcc gggaatgccg
     2521 aagccggtga tcgccagggt gccgatggtc atcaccgcat aggtcaccgg caggtgcttc
     2581 cacagcccgc ccatcttccg catgtcctgc tcgtggtgca tgccgtggat caccgagccg
     2641 gcgccgagga acagcagggc cttgaagaag gcgtgggtga acaggtggaa catcgccgcc
     2701 tggtaggccc cgacgccggc ggcgaagaac atgtagccga gctgcgaaca ggtcgaatag
     2761 gcgatgaccc gcttgatgtc gttctgggcc aggccgaccg tcgccgcgaa cagggcggta
     2821 atcgccccga tcagggtcac gacgttgcgc gccatcgggg cgtactcgaa catcggcgac
     2881 agcaggcaga ccatgtagac gccggcggtg accatggtcg ccgcgtggat cagggccgag
     2941 accggcgtcg ggccttccat ggcgtcgggc agccaggtgt ggaggaagaa ctgcgccgac
     3001 ttgcccatgg cgccgatgaa cagcagcacg caggccaggt cgaccagcgg ccagctctgg
     3061 ccggcgaagg tccaggtctt cgaggcgctg tccgcgatca tcgggaacag ctcggcgaac
     3121 tggatggtgc cgtaggccca gaacaccgtc atgatgccca gggcgaagcc gaagtcgccg
     3181 acgcggttga ccacgaaggc cttgatcgag gcggcgttgg ccgtcggttt cttgaaccag
     3241 aaaccgatca gcagatagga cgccaggccc accccttccc agccgaagaa cagctgcatg
     3301 aagtcggcgg ccgtcaccag cgccagcatg gcgaaggtga acagcgacag gtaggcgaag
     3361 aaccgcggcc ggctctcgtc ctcggccatg tagccccagc tgtagagatg cacgagggcc
     3421 gagacggtgg tcaccaccac cagcatgacc gcgctcagcc cgtcgatgcg gatggaccag
     3481 gcagactgga aatcgccgac attgatgaac ggggccagtg acacggtgaa cggctcgagg
     3541 ccgccccagg tccactggct gaaggtgtac caactgaggc cgcaggccag cagcagcagg
     3601 cccgtggtga cgctctgcga cgccagcgcg ccgatgcggc gaccgaaaag gccggcgacc
     3661 agggcgccca gcagcggagc gaagagaaga acggtgacga gctgttgcaa gggtcagccc
     3721 ttcatcacgg aggcgtcgtc cacggcgatg tcgccgcggt tgcggaagaa ggtcaccagg
     3781 atggccaggc cgacggcggc ctcggccgcg gcgacggtca gcacgaacat ggcgaagatc
     3841 tgcccgacca cgtcgtgcag gaagacgctg aaggcgacca ggttgatgtt caccgccagc
     3901 aggatcagct cgatcgacat caggataatg atgacgttct tgcggttcac gaagatgccg
     3961 aagacgccga tcgtgaacag gatcgcggcg acggtcaggt agttggtcag accaatcatt
     4021 cgctgatccc ctcgccgggc ttgatgtcga cgatcgtcat gccggtggcc ggcgtgcgcg
     4081 cgttctgggc tgcgatgttc tggcgcttga ccccgggctt gtggcgcagg gtcagcacga
     4141 tggctccgat catcgccacc agcagcacca gaccggccgc ctggaagaaa tagacgtagt
     4201 cggtgtagag cacccggccg atggtctcgg cgttggtcac atccgtcccg gcggaggcgt
     4261 cgggtcctgg agaggccgat gccgcgccct ggctggccac cgtggtggcg acgatgatca
     4321 tctcgacggt caggatgccg gcgaccagcc cgccgaccgg caggtagcgc gaaaaaccct
     4381 cgcgcagctg ggtgaagtcc acgtccagca tcatcacgac gaacaggaac agcaccgcca
     4441 ccgcgccgac gtagaccacg accagcagca tggcgaggaa ctcggcgccc atcagcacga
     4501 acagtcccgc cgcgctgaag aaagccgcga tcaggaacag caccgagtgc acgggattgc
     4561 gcgcgaagac cacggcgagt gccgccgaaa tggtcgtcgc cgccatgatg tagaaggcta
     4621 tcgcctgcag agccatctgg gcccctgctc cctcttcaac ccttgcgaaa gaggcggcgc
     4681 agatgagctc cgccgcctcc ggtgaatcgc cgcctcccct tatcggtagg cagcgtccag
     4741 ttccagattc ttcgcgatct cgcgctccca gcggtcgccg ttatccagca gccgctcctt
     4801 gtcgtagtag agctcttccc gcgtctcggt ggcgaattcg gtattcggcc cctcgacaat
     4861 ggcgtcgacc ggacaggcct cctgacagag gccgcagtag atgcacttga ccatgtcgat
     4921 gtcgtagcgg gtcgtacgcc ggctgccgtc ggcccgcggt tcggcctcga tatagatggc
     4981 ctgggccggg cagacggctt cgcaaagctt gcaggcgatg cagcgttcct ctcccgaggg
     5041 atagcggcgc agggcgtgct cgccccggaa acgcggcgac tgcggaccgc gctcgttggg
     5101 atagatgacc gtcttcttcg gcgcgatcat gtacttcatg gccagtccga aggcgcccac
     5161 gaagtccagc agggccgcgc ctttgatggc ctgtccgatg cg
//
LOCUS       DAORNX010000185         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11827_length_5202_cov_6.881290, whole genome shotgun sequence.
ACCESSION   DAORNX010000185 DAORNX010000000
VERSION     DAORNX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11827_length_5202_cov_6.881290"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLO65_09125"
     CDS             <1..464
                     /locus_tag="PLO65_09125"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRD28448.1"
                     /translation="YFKSKDDILFECHRIGIDAIINGDKPMPTAAESSGRERLVEFLR
                     RYVRMVVDDFGTCLVMTGTNALEPENQGTVIEGRRQIDSMLREILDDGVRDGSLDCPD
                     PKLTAMFVFGAMNWIPRWYHLDGTLTVDELTERLTAFVLRAIGPARVNAPA"
     gene            519..1703
                     /locus_tag="PLO65_09130"
     CDS             519..1703
                     /locus_tag="PLO65_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HRD28449.1"
                     /translation="MSMQRVVVAGVGMTAFVKPGASAPYDEMARDAGRMALADAGIDY
                     AAIQQVYAGYVYGDSTCGQAAAYQLGMTGAPVINVNNNCATGSTALFLARQAVASGAA
                     DCVLALGFEQMRRGALVDVWEDRRSPLAPFIEAMAGARGEADVPLAFYLFGGAGSEYQ
                     ERYGVRPETFAKVTVKARRHAVHNDRAIFRTEVSVEEVLASPRLYGPLTRLQACPPTC
                     GAAAAVICSEAYARKHGINADVVIAGQAMTTDFPTALNSGSAVQIIGYDMAAAAARQA
                     YEMAGIGPGDAQVAEMHDCFTANEVVSYEALGLAPAGGAEKFIEDGDNTYGGRIVVNP
                     SGGLISKGHPLGATGLAQCTELVQQLRGTCGPRQVEGARTAIQHNLGLGGACVVTVYQ
                     RA"
     gene            1889..3295
                     /locus_tag="PLO65_09135"
     CDS             1889..3295
                     /locus_tag="PLO65_09135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD28450.1"
                     /translation="MSRLIPVLALGLLAAACDRGPPAPILSIASGSVTHSVCTAEFVS
                     GRGAADLRRQEFSGLYGLALLLTRPKIDRAARTVTGRGPFGATAMAVVRPGLGCILLS
                     GRTPEDVKAFRADGPTGPAPGPAAVWPAGERVDLTAPIPGVDRAKLDLAMDTAFGRCP
                     KGNDPFTRAIVVVHGGRIVAERYGKGFDAASRPYGASMSKSVAATLLGLRIAEGRMAL
                     DGVALPPGPGRPDPVSNRDLVTMTAGLAFNEDYDADSDVNRMLMMEPDMAAAAARHPA
                     YARPGAVWKYSSGATNILMRSLRDSFAGDDQAYWNYPRKALFDPLGMRSAIFQTDASG
                     TLVGSSFMWATPRDWARLGLLYLNRGRWNGRQLLPGSWVDYVRTPSPPTAALKGDDHL
                     TYGAGFWLDGADGVAPAPVYAMGGWAFQKVRIEPARDLVIVRMGHTLKDCPSASIEPI
                     AALFPKVAVSPARPTARS"
     gene            3415..4464
                     /locus_tag="PLO65_09140"
     CDS             3415..4464
                     /locus_tag="PLO65_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD28451.1"
                     /translation="MSPSPLLGTPVAPMSESEAEALALDIFGVAGSARPLTSERDQNF
                     LLTTPDAGRLVLKIANPAEDPAVTRFQAAALAHVAEVDPALPTPRVVRTREGEISTLL
                     TRPGEGPRVVRMLTFLPGTMVHEVKSTPALRRNIGAAHARLGLALAGFDHPAADHDLP
                     WDMKRAGRLAELRDSIPEPSRRALVDEAMDRFRTRVEPRLGDLRSQVVHNDLNQHNLV
                     VDPDHPDRVAGILDFGDMVRAPLACDVAVAAAYMLSDGDDPLEGAAPYVAGYHAVSPL
                     RPEEADLLADMVATRLLMTVLITGWRAEVHPHNRAYILRNNGPAWSRLEKLMARDRGA
                     GDDPLRSACTGGTLS"
     gene            4461..>5202
                     /locus_tag="PLO65_09145"
     CDS             4461..>5202
                     /locus_tag="PLO65_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD28452.1"
                     /translation="MTMVNAFGGDAAPDLPPAERALIERRARVLGPAYRLFYQRPVHI
                     VRGEGVWLYDPAGAAYLDVYNNVASTGHCHPHVVAALSRQAATLNTHTRYLNETVVDY
                     AERLTALFPEALSQVMFTCTGSEANDLALRVARARTGATGVIVTATAYHGVTAAVAEA
                     SPSLGDGVPLGVNVRAVPPPDGSDNGERFAAGVAAAIADLAAHGQGVAALLADTLFSS
                     DGVVSDPPGFLKPAVAAVRRAGGLFIADE"
BASE COUNT          756 a         1867 c         1815 g          764 t
ORIGIN      
        1 attatttcaa gagcaaggac gacatcctct tcgagtgcca ccgcatcggc atcgatgcga
       61 tcatcaacgg cgacaagccg atgccgacgg cggccgagag ctcaggacgc gagcggctgg
      121 tcgagtttct gcgccgctac gtgcgaatgg tcgtcgacga ctttggcacc tgtcttgtga
      181 tgaccggcac caacgccctg gagccggaaa accaggggac ggtcatcgag ggccgccggc
      241 agatcgactc gatgctgcgc gagattctcg atgacggcgt gcgggacggc agcctcgact
      301 gccccgatcc caagctgacg gcgatgttcg tgttcggcgc catgaactgg attccgcggt
      361 ggtaccacct cgacggcacc ctgaccgtcg atgagctgac cgagcgcctg accgccttcg
      421 tgttgcgggc catcggtccg gcccgggtga acgctccggc gtagcggaag accggctgct
      481 gcgtgtctcc acccctgacg ggaagcgcgg ggtatcttat gagcatgcag cgagtggtgg
      541 tggccggcgt cggcatgacg gccttcgtca agccgggggc ctctgctccc tatgacgaga
      601 tggcgcggga cgccgggcgg atggccctgg ccgatgccgg gatcgactat gccgccattc
      661 agcaggtcta cgccggctat gtctacggcg actcgacctg tggccaggcg gcggcctacc
      721 agctgggcat gaccggggcg ccggtgatca acgtcaacaa caactgcgcg accggctcga
      781 ccgcgctgtt cctggcccgt caggcggtgg cgtcgggcgc ggccgactgc gtgctggcgc
      841 tgggcttcga acagatgcgc cgcggcgccc tggtcgacgt ctgggaggac cgtcgcagcc
      901 cactcgcgcc gttcatcgag gccatggccg gcgcccgggg cgaggccgat gtcccgctgg
      961 ccttctatct gttcggcggg gcagggtccg aataccagga acgctatggc gtccggccgg
     1021 agaccttcgc caaggtgacc gtcaaggcgc gtcgccatgc ggtgcacaat gacagggcga
     1081 tcttccgcac cgaggtgtcg gtcgaggagg tgctggcctc gccgcgcctg tatggtccgc
     1141 tgacccggct ccaggcctgc cccccgacct gcggcgcggc ggcggcggtc atctgcagcg
     1201 aggcctacgc ccgcaagcac ggcatcaatg cggatgtggt catcgccggc caggcgatga
     1261 ccaccgattt ccccaccgcg ctcaactcag gcagcgcggt gcagatcatc ggctacgaca
     1321 tggccgccgc cgccgcacgc caggcctacg agatggccgg catcggaccg ggggacgccc
     1381 aggtcgccga gatgcacgac tgcttcaccg ccaacgaggt ggtcagctac gaggcgcttg
     1441 gcctggctcc cgccggcggc gcggagaagt tcatcgagga cggcgacaat acctacggcg
     1501 gccgcatcgt ggtcaatccg tcgggcggcc tcatttccaa gggccatccg ctcggcgcca
     1561 cgggcctggc ccagtgcacg gaactggtcc agcagttgcg cgggacctgt ggcccgcgcc
     1621 aggtcgaggg cgcccgcacg gccatccagc acaacctcgg cctgggcggc gcctgtgtcg
     1681 tgacggtcta tcagcgagcc tgagcggtgc tcgggaccga agggatcggt cgcacgctcg
     1741 agccctgccc cggcgcgtcg aggctggccg gggcatgccc gacggtgcgg ccgctgcggt
     1801 tgatcacttc cgcgcaccac atcaacagcg agatcctggt cgccgccgac gggatgttga
     1861 tgagacccgg cccaacggag gaacacccat gtcccgactg attccggtgc tcgcgctcgg
     1921 cctgctggcg gcggcctgcg accgtgggcc gcccgcgccg atcctctcga tcgcatcggg
     1981 ctcggtcacc cattccgtct gcacggccga gttcgtctcc gggcgcggcg ccgctgacct
     2041 gaggcgccag gagttctcgg gcctctacgg tctcgcgctg ctgctgacgc gtccgaagat
     2101 cgaccgggcg gcgcggaccg tcaccgggcg cgggccgttc ggcgcgaccg ccatggcggt
     2161 ggtccggcca gggctgggct gcatcctgct cagcggccgg acgcccgagg atgtgaaggc
     2221 gttcagggct gacggaccga ccggcccggc tcccggtccc gccgccgtct ggcccgcggg
     2281 cgagcgcgtc gatctgaccg cgccgatccc cggagtcgac cgggcgaagc tggacctggc
     2341 catggacacc gccttcggac gctgtcccaa ggggaacgac cccttcaccc gggcgatcgt
     2401 tgtggtccac gggggccgca tcgtcgccga gcgctacggc aagggcttcg acgccgccag
     2461 ccggccctat ggcgcctcca tgtccaaaag cgtggcggcg acgctgctgg gcctgcgcat
     2521 cgccgagggc cggatggcgc tcgacggcgt cgcgctgccg ccgggacccg ggcggccgga
     2581 tccggtcagc aaccgcgacc tggtcaccat gaccgcggga ctggcgttca acgaggacta
     2641 cgacgccgat tccgacgtca accgcatgct gatgatggag ccggacatgg ccgccgccgc
     2701 cgcccgccac ccggcctacg ccaggcccgg cgcggtgtgg aaatactcca gcggcgccac
     2761 caatatcctg atgcgctccc tgcgcgacag cttcgccggc gacgaccagg cctactggaa
     2821 ctatccgcgc aaggcgctgt tcgacccgct cggcatgcgg tcggcgatct tccagacgga
     2881 tgcgtccggc accctggtgg ggtcgagctt catgtgggcg acgccaaggg actgggcgcg
     2941 acttggcctg ctctatctca atcgcggacg gtggaacggc aggcagctgt tgcccgggag
     3001 ctgggtcgac tacgtgcgca ccccgtcccc gccgacggcg gcgctgaagg gcgacgatca
     3061 cctgacctac ggggccggct tctggctcga tggcgccgac ggcgtcgcgc cggcgccggt
     3121 ctatgcgatg ggcggctggg cgttccagaa ggtgcgcatc gagccggcgc gtgaccttgt
     3181 cattgtgcga atgggccaca cgctcaagga ctgtccgagc gccagcatcg agccgatcgc
     3241 ggccctgttt ccgaaagtcg cggtcagccc ggcccgacca acggccaggt catgacgatc
     3301 ccgtcagcct ccctggccgg agagtggccc gaagccggcc cgggcgacag cacgccgttg
     3361 ccggggccgc ccatcgaaca cagagtaggg acgacgagtc cgaaggatcc cgccatgtcg
     3421 ccaagcccct tgctgggaac tccggtcgcg ccgatgtcgg aatcggaggc cgaggccctg
     3481 gcgctcgaca tcttcggcgt ggccggatcg gcccggccgc tgaccagcga gcgcgaccag
     3541 aacttcctcc tgaccacgcc cgacgccggg cggctcgtgc tgaagatcgc caatcccgcc
     3601 gaggacccgg cggtgacccg gttccaggcc gccgccctgg cccatgtggc cgaggtcgac
     3661 ccggccctgc cgaccccccg cgtggtccgc acccgcgagg gcgagatcag caccctcctg
     3721 acccgccccg gcgaggggcc gcgcgtcgtg cgcatgctga ccttcctgcc cgggacgatg
     3781 gtgcatgagg tgaaatcgac gccggccctg cgacgcaaca tcggggccgc gcacgcgcgc
     3841 ctcggcctcg ccctggccgg cttcgaccat ccggccgccg accatgacct gccgtgggac
     3901 atgaagcgcg ctggtcggct ggccgagctg cgcgactcga ttcccgagcc gtcccggcgc
     3961 gccctcgtcg acgaggccat ggaccgtttc aggacccgcg tcgaaccgcg actcggcgac
     4021 ctgcgcagcc aggtggttca caacgatctc aaccagcaca acctggtggt cgatcccgac
     4081 catcccgacc gggtcgccgg catcctcgac ttcggcgaca tggtccgggc gccgctggcc
     4141 tgcgacgtgg ccgtagcggc cgcctacatg ctgagcgacg gcgacgatcc gctggagggc
     4201 gccgcgccct atgttgcggg ctatcacgcc gtctcgcccc tgcggccgga ggaggccgac
     4261 ctgctggcgg acatggtcgc gacacgcctg ctgatgaccg tgctgatcac cggctggcgg
     4321 gccgaggtcc atccgcataa ccgcgcctat atcctgcgca acaacggccc ggcctggtcg
     4381 cggctggaaa agctgatggc ccgtgaccgt ggcgcgggcg acgatccgct gcgatcggcc
     4441 tgcacggggg gaacgctgtc atgaccatgg tcaacgcctt cggcggcgac gccgcccccg
     4501 acctgccgcc cgccgaacgc gccctgatcg agcggcgcgc cagggtgctg ggcccggcct
     4561 atcgtctgtt ctaccagcgg ccggtccaca tcgtgcgcgg ggagggggtc tggctctacg
     4621 atcccgccgg cgcggcctat ctcgacgtct acaacaacgt cgcctcgacc ggccattgcc
     4681 acccgcatgt ggtggccgct ctgtcgcgcc aggcggcgac cctgaacacc cacacccgct
     4741 atctcaacga gacggtggtt gactacgccg agcggctgac ggcgctgttc cccgaggccc
     4801 tgagccaggt gatgttcacc tgcacgggca gcgaggccaa cgacctggcc ctgcgtgtcg
     4861 cccgggcccg gacgggcgcg acgggcgtga tcgtcaccgc caccgcctat cacggcgtca
     4921 ccgccgcggt ggccgaagcc tcgccctcgc tgggcgacgg cgtgccgctg ggggtcaatg
     4981 tccgggccgt gccgccgccg gacgggtcgg acaacggcga gcggttcgcg gccggggtgg
     5041 cggcggccat cgctgacctc gccgcccacg gacagggagt cgccgccctg ctggcggaca
     5101 ccctcttctc aagcgacggg gtggtgtccg atccgcccgg attcctgaag ccggccgtgg
     5161 cggccgtgcg gcgggccggc ggcctgttca tcgccgacga gg
//
LOCUS       DAORNX010000186         5199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11833_length_5199_cov_8.492224, whole genome shotgun sequence.
ACCESSION   DAORNX010000186 DAORNX010000000
VERSION     DAORNX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11833_length_5199_cov_8.492224"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="eno"
                     /locus_tag="PLO65_09150"
     CDS             complement(<1..514)
                     /gene="eno"
                     /locus_tag="PLO65_09150"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517168.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD28453.1"
                     /translation="MTEIVDIIAREILDSRGNPTVEVDVILEDGSFGRAAVPSGASTG
                     AHEAVEKRDGDKNRYGGKGVLDAVAAVNGEIYDALGGVDAEDQRRIDSLLIELDGTKN
                     KARLGANAILGVSLATAKAAAVSADLPLYKYVGGVSARVLPVPMMNIINGGAHADNPI
                     DIQEFMILPTG"
     gene            776..1552
                     /locus_tag="PLO65_09155"
     CDS             776..1552
                     /locus_tag="PLO65_09155"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD28454.1"
                     /translation="MLLLVEKQDGIAVVTLNRPEAMNALSKAMRSALYKAMVELDADP
                     EVSVVILTGAGERAFTAGLDLKELGSDPKAMSAANAEGADENPVKAIELCRKPVIGAI
                     NGVAITGGFEVALACDVLIASENARFADTHARVGIMPGWGLSQKLSRLIGIYRAKELS
                     LTGNFLDATTACAWGLVNRVVPAADLLTVARKLAADMATIPVETLTTYKRIIDGGYAL
                     AYGDSLKFEAEKSTANNRLVTPEAVEARRAGIQARARTQG"
     gene            complement(1549..2484)
                     /locus_tag="PLO65_09160"
     CDS             complement(1549..2484)
                     /locus_tag="PLO65_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRD28455.1"
                     /translation="MLSRRQLALALAGALTPAPVFAQDAPPPIDPEETIGAGEDRYDR
                     LTIPVRIGDSGPFEFVVDTGADRTVLSQEVADQLGLPRGRDVMVQGITGSELTPTVKA
                     PPINLGRIVLNGLDLPVLPRQRLGVDGLLGVDALQKRRLVMDFRERRLEILPASGAFR
                     PGSTSRETYVPARDKFGRLVVIDANANNAPVAAFIDSGAGMSIANRALAESIRSRGGW
                     RGEGRTVPLYGVTSHQATGELRLLDSVRMGGLRFTDIPVLISDLDLFEQWGMATRPTI
                     LLGVNVLRLFSRVEMDYGRRRVMFRVAADPQIWLA"
     gene            complement(2610..2792)
                     /gene="rpmF"
                     /locus_tag="PLO65_09165"
     CDS             complement(2610..2792)
                     /gene="rpmF"
                     /locus_tag="PLO65_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRD28456.1"
                     /translation="MAVPKRKVSPSRRNMRRSHHALGANSYVEDKDTGELRRPHHVDL
                     KTGMYGGRQVITPKED"
     gene            complement(2904..3446)
                     /locus_tag="PLO65_09170"
     CDS             complement(2904..3446)
                     /locus_tag="PLO65_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRD28457.1"
                     /translation="MRTCGSCNLCCKVLEVRSLAKPAGQWCEHAIRGRGCGIHGRHPA
                     DCRAFTCGWLQSSDLGPEWKPEICRFVLRDESDERRLCVDVDPGFPDAWKKPAYYPLI
                     KRWSGAVRDRTGCVLVYVGARTIVVFPEEDIDLGPLEPAPVLVVGYLKTRDVRRPLVR
                     HMVDGAVAAEWRGAPVTPGR"
     gene            complement(3448..5097)
                     /locus_tag="PLO65_09175"
     CDS             complement(3448..5097)
                     /locus_tag="PLO65_09175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRD28458.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVNATRADNITTGQIYKTIIEKE
                     RRGDYLGATVQVIPHVTNEIKDFVLAPAVDENGAEADFVLVEIGGTVGDIEGLPFFEA
                     IRQLGQELPRGSSCFVHLTLLPFIKTAGEMKTKPTQHSVKELRSIGIQPDILLCRCEQ
                     PIPADEKRKIGQFCNVRPSAVIQAMDSASIYAVPLDYHVQGLDTEVLDVFGITDAPAP
                     DLSRWQTISETLTNPDGEVTIAVVGKYTVLKDAYKSLIEALSHGGVANRVKVNLDWVE
                     SETFENQDGAAASRLEGVHGILVPGGFGERGSEGKILAAEFARVHNVPYFGICFGMQM
                     AVIEALRNVAGVAGASSTEFGPTAEPVVGLMTEWTQGNQRIERRAGDDLGGTMRLGAY
                     EAVLSPGSRVADIYGSTDIFERHRHRYEVNIGYREQMESAGLMLTGCSPDGILPEIVE
                     RADHPWFVGVQFHPELKSRPFAPHPLFASFIAAAKEQSRLV"
BASE COUNT          828 a         1753 c         1806 g          812 t
ORIGIN      
        1 cgccggtcgg caggatcatg aactcctgga tgtcgatcgg attgtcggcg tgggcccccc
       61 cgttgatgat gttcatcatc ggcacgggca gcacccgggc cgagacgccg ccgacgtact
      121 tgtagagcgg caggtcggcg ctgacggcgg cggccttggc cgtggccagg gagacgccga
      181 ggatggcgtt ggcgcccagg cgggccttgt tcttcgtgcc gtccagctcg atcagcaggc
      241 tgtcgatgcg acgctggtcc tcggcgtcga cgccgcccag ggcgtcgtag atctcgccgt
      301 tgaccgcggc gaccgcgtcc agcacgccct tgccgccgta gcggttcttg tccccgtcgc
      361 gcttctcgac cgcctcgtgg gcgcccgtgg aggcgcccga cggcaccgcc gcccggccga
      421 acgaaccgtc ctccaggatc acgtcgacct cgaccgtcgg gttgccgcga ctgtcgagga
      481 tttcgcgggc gatgatgtcg acgatctcgg tcatgggagc tccggcgggc aaggtcttga
      541 aaaggcgcgc cccgattagc gccctgaccg tggaatcgca actgaagggc cgttgcgtgg
      601 ttcgacacgc acccttcggg tgctgctcac catgacgtgg aatttggcgg cccacccctg
      661 cacgtcatcc tgagcaggcc caaagggccg tgtcgaagga tgcatcctgc aacgcggccg
      721 gcttgactgc ccctccgtcg cccggtcatg cccagaccaa acggagaacc accccatgct
      781 gctgctggtc gagaaacagg acgggatcgc cgtcgtcacc ctgaaccgcc ccgaggcgat
      841 gaacgccctc agcaaggcga tgcgcagcgc gctgtacaag gccatggtcg agctggacgc
      901 cgatccggag gtcagcgtgg tgatcctgac cggcgcgggc gagcgggcct tcaccgccgg
      961 cctggacctc aaggagctgg gctccgaccc gaaggcgatg agcgcggcca acgccgaggg
     1021 ggccgacgag aacccggtca aggccatcga actgtgccgc aagccggtga tcggggcgat
     1081 caacggggtg gccatcacgg gcggcttcga ggtggccctg gcctgcgatg tgctgatcgc
     1141 ctcggagaac gcccgcttcg ccgacaccca tgcgcgggtc ggcatcatgc cgggctgggg
     1201 cctgtcgcag aagctgtcgc gcctgatcgg catctaccgg gccaaggagc tctccctgac
     1261 cggcaacttc ctcgacgcga cgaccgcctg tgcgtggggc ctggtcaacc gggtcgtgcc
     1321 ggcggcggac ctgttgaccg tggcgaggaa actggccgcc gacatggcca ccatccccgt
     1381 cgagaccctg accacctaca agcggatcat cgacggcggc tatgccctgg cctacggcga
     1441 cagcctgaag ttcgaggccg aaaagtccac cgccaacaac cggctggtca cgcccgaagc
     1501 cgtcgaggcc cgtcgcgccg gcattcaggc gcgggcgcgg acccagggct aggccagcca
     1561 gatctgcggg tcggccgcga cgcggaacat cacccgccgc cggccgtaat ccatctccac
     1621 ccgggagaac agccgcagca cgttgacccc cagcaggatc gtcgggcggg tcgccatgcc
     1681 ccactgctcg aacaggtcca ggtcgctgat cagcaccggg atgtcggtga aacgcaggcc
     1741 gcccatgcgg accgaatcga gcaggcgcag ctccccggtg gcctggtggc tggtcacgcc
     1801 gtagagcggc accgtccggc cttcgccgcg ccagccgccg cgggagcgga tcgactcggc
     1861 cagggcccgg ttggcgatgc tcatgcccgc gccggaatcg atgaaggcgg cgacgggagc
     1921 attgttggcg ttggcgtcga tcaccaccag ccgaccgaac ttgtcgcggg ccggaacata
     1981 ggtctcgcgg ctggtcgagc cgggccggaa ggcgccggag gccggaagga tctccagccg
     2041 tcgctcgcga aagtccatca ccagccgccg cttctgcagg gcgtccaccc ccagcagacc
     2101 atcgacgccg agccgctggc gcggcagcac cggcaggtcc agcccgttga ggacgatccg
     2161 gccgaggttg atcggcggcg ccttgaccgt cggggtcagt tccgagccgg tgatgccctg
     2221 gaccatcacg tccctgcccc gcggcaggcc cagctggtcg gccacttcct gcgacaggac
     2281 ggtgcgatcc gcgccggtat cgaccacgaa ttcaaagggg ccgctgtcac cgatccggac
     2341 gggaatggtc aggcgatcgt agcgatcctc gccggccccg atggtctctt cgggatcaat
     2401 gggcggcggg gcgtcctggg cgaagaccgg ggcgggcgtc aaggcgccgg ccagggccag
     2461 agcaagttgt cgacggctca gcatggggtc ctccccccgt attttcgggg aggatagcac
     2521 gggccggaac ggcggtcgcc caaatgcgaa cggcgcccgg aaggatcccc cgggcgccgt
     2581 cgaagtccgt cagaccgtcg ctgaagcgac tagtcttcct tgggcgtgat gacctggcgg
     2641 ccgccgtaca tgccggtctt cagatcgaca tggtgcgggc ggcgcagctc gccggtgtcc
     2701 ttgtcctcga cgtagctgtt ggcgcccagg gcgtggtgcg accggcgcat gttgcgacgc
     2761 gacggagaga ctttgcgttt gggaacggcc atcggataac tctgctcgct gagcatgaaa
     2821 acggtgacgg cggccgtcgg gccgccgcgt gaggcggggt atatgcccca agagttcctc
     2881 gagttcaagc cgctgcgtcg gtctcagcgg cctggcgtga cgggagcccc ccgccattcg
     2941 gcggcgaccg ctccgtcgac catgtgacgc accagcgggc ggcggacgtc gcgggtcttc
     3001 agatagccga cgaccagcac gggcgccggc tcaagcggcc ccaggtcgat atcctcctcg
     3061 ggaaacacca cgatggtcct ggcgccgaca tagaccagca cgcagccggt ccggtcgcgg
     3121 accgcccccg accaccgctt gatgagcgga tagtaggccg gcttcttcca ggcgtccgga
     3181 aagccggggt cgacgtcgac gcacagccgg cgttcgtccg attcgtcgcg caggacgaaa
     3241 cgacagatct cgggcttcca ctcggggccc aggtcggacg actgcagcca gccgcaggtg
     3301 aaggcgcgac agtcggccgg gtggcgcccg tggatgccgc agccccggcc cctgatcgcg
     3361 tgttcacacc actggcccgc cggcttggcc agcgaccgga cctccaggac cttgcagcag
     3421 aggttgcagg atccgcaggt ccgcatccta gaccagccgg ctctgctcct tggccgccgc
     3481 gatgaagctg gcgaacaggg ggtgcggggc gaacgggcgg ctcttcagct ccggatggaa
     3541 ctgcaccccg acgaaccagg ggtggtcggc ccgctcgaca atctcgggca ggatgccgtc
     3601 aggcgaacag cccgtcagca tcaggccggc cgactccatc tgctcgcgat agccgatatt
     3661 gacctcgtag cgatggcggt gccgttcgaa gatgtcggtc gagccgtaga tgtcggccac
     3721 ccgggagccg ggcgacagca cggcttcata ggcccccagg cgcatggtgc cacccaggtc
     3781 gtcgccggcg cggcgctcga tccgctggtt gccctgggtc cattcggtca tcagcccgac
     3841 aaccggctcc gccgtggggc cgaattcggt ggaggacgcg ccggcgacgc cggccacgtt
     3901 ccgcagcgcc tcgatgaccg ccatctgcat gccgaaacag atgccgaaat agggcacgtt
     3961 gtgaacgcgg gcgaactcgg ccgcgagaat cttgccctcg ctgccccgct cgccgaatcc
     4021 gccgggcacg aggatgccgt gcacgccctc gagccggctg gccgcggcgc cgtcctggtt
     4081 ctcgaacgtc tcgctctcga cccagtcgag gttgaccttg acccggttgg cgacgccgcc
     4141 gtggctgagc gcctcgatca gggatttgta ggcgtccttg agcaccgtgt acttgcccac
     4201 cacggcgatg gtcacctcgc cgtcgggatt ggtcagggtc tcgctgatgg tctgccagcg
     4261 cgacaggtcc ggcgccggcg cgtcggtgat gccgaagaca tccagcacct cggtgtccag
     4321 cccctgcaca tggtagtcga gcggcacggc gtagatcgag gccgagtcca tggcctggat
     4381 cacggcgctg ggccgcacgt tgcagaactg gccgatcttg cgcttctcgt cggccgggat
     4441 cggctgctcg cagcggcaca gcaggatgtc cggctggatg ccgatcgaac gcagctcctt
     4501 gaccgagtgc tgggtcggct tggtcttcat ctcgccggcc gtcttgatga acggcagcag
     4561 ggtcaggtgg acgaagcagc tgcttccgcg cggcagttcc tggccgagct ggcggatcgc
     4621 ctcgaagaat ggcaggccct cgatgtcgcc gaccgtgccg ccgatctcga ccagcacgaa
     4681 atcggcctcg gcgccgttct cgtcgaccgc cggcgccagg acgaaatcct tgatctcgtt
     4741 ggtgacgtgc ggaatcacct ggaccgtcgc gccgagatag tcgccgcgcc gctccttttc
     4801 gatgatggtc ttgtaaatct ggccggtggt gatgttgtcg gcgcgggtgg cgttgacgcc
     4861 ggtgaagcgc tcgtagtggc ccaggtcgag gtcggtctct gcgccgtcgt cggttacgaa
     4921 gacctcgccg tgctgatagg ggctcatcgt ccccggatcg acgttcagat aggggtcgag
     4981 cttgcgcagg cggaccttgt agccacgcgc ctggaggagc gcgccaaggg cggcggacgc
     5041 gagacctttt cccagggagg agaccacgcc gccggtgatg aaaatgtacc gggccatggg
     5101 aatccagaat agcgccgatt cggggtcggc gcctgctcca tcgacggggt gacgaaacta
     5161 gttggcgggc ggggcgtccg acggagcgac gccgccgag
//
LOCUS       DAORNX010000187         5182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11884_length_5182_cov_6.458748, whole genome shotgun sequence.
ACCESSION   DAORNX010000187 DAORNX010000000
VERSION     DAORNX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11884_length_5182_cov_6.458748"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLO65_09180"
     CDS             complement(<1..308)
                     /locus_tag="PLO65_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HRD28459.1"
                     /translation="MTDAAAARSLLSAAAVRERAHEMLAIALDGGLTDWRVNLDRLPH
                     TADVVASVIRDQYPTLAVPFHARWRHFVVGGRDLWAELAAQADWPDAKAKARAAFDLA
                     "
     gene            complement(404..1660)
                     /locus_tag="PLO65_09185"
     CDS             complement(404..1660)
                     /locus_tag="PLO65_09185"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF005536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRD28460.1"
                     /translation="MSVNRQGHIRLTSHPGSGSPGRFPLAWGAPTARERGPVVATVNA
                     GNDRNAIGAHGGAYSIYRSLAIASGALNPLVRPDLTDTAPVVDVGPFPQWADPHRIVS
                     LDPWGHRIQQDFAAEVAEGVDIRPSIAITKARLTLPEIGEAIDAGRLRIDGTVVHETR
                     DVAVTKAALDPVWYLPGIAERFEVDETTLRRTLFEQTGGMYPELVTRPDIKVFLPPIG
                     GITLYIFGDPAAIADPARRLTCRVHDECNGSDVFGSDICTCRPYLIHGIEEAVLEAQN
                     GGAGLIVYNRKEGRALGEVTKFLVYNARKRQPGGDQAATYFERTECVAGVQDARFQQL
                     MPDVLHWLGIKHIDRFVSMSNMKHDALVEGGITIGERVPIPAWLVPDDASVEMEAKKA
                     AGYFTPDGAPTAEDLKKTVGRDLGEF"
     gene            complement(1792..1986)
                     /locus_tag="PLO65_09190"
     CDS             complement(1792..1986)
                     /locus_tag="PLO65_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28461.1"
                     /translation="MRTYAIYIRDVRYSVPSLLLVEAAAEERVADIARQKLEESPHHL
                     AVEVVEGDTVLFRFQRADGE"
     gene            2106..3116
                     /locus_tag="PLO65_09195"
     CDS             2106..3116
                     /locus_tag="PLO65_09195"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRD28462.1"
                     /translation="MTDTIEDFIRGLPKAELHLHIEGSLEPEQMFEFGRRNRVDMPFN
                     SVEEVRAAYAFSNLQDFLDIYYQGAAVLRTEQDFHDMAMAYFRRLHADGGVHAEIFFD
                     PQTHTDRGLPFSVAIEGLLSGMKAAEAELGVTSGLIMCFLRHLDEDSAMATLKASEPW
                     LDRLVGVGLDSSEVGHPPSKFQRVFRAAKERGLTLSAHAGEEGPPAYVWEALDLLKVD
                     RIDHGNRALEDPALVQRLVAGEMTLTVCPLSNLKLCVVNDLTAHPMKRMLDLGLKATC
                     NSDDPAYFGGYIGENFIQTARALDLSRDEIVTLAKNSFTGSFLDEERVAHHLASIDAH
                     AA"
     gene            complement(3198..4004)
                     /locus_tag="PLO65_09200"
     CDS             complement(3198..4004)
                     /locus_tag="PLO65_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28463.1"
                     /translation="MKTRTIVLTLTSVLALSAGAAIAQNVPTWEEYEAQQRAYEQQQG
                     AYQQQREDYQDRQQAYADQRRAYETKRAQYLRDRAAYDRRYGVGAYERRYGAWRWDPG
                     PYAYQPYDSRYPYDAGYYGRDVSARVDVDRYRNSPCEQRRSGNTAAGLIIGALAGAAI
                     GSNVAATDVQTEGAVLGAVVGGGLGASIGRDSARCDDRGYWYSRDETIPYREARYYRD
                     RRARSGRYEYSYYERRGCRLAPAPAEYAGRVEYRYVRVCPDSRGRYRITR"
     gene            complement(4170..>4788)
                     /locus_tag="PLO65_09205"
     CDS             complement(4170..>4788)
                     /locus_tag="PLO65_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28464.1"
                     /translation="TWRTAGLCGTGSLDFQVKDVRVPKARSVALQSDPPTVNTPLGRF
                     PVFGMLALGVTAVALGNARAALLTAGSAAQTKKASGSQRALAERNVVQADFARAVARL
                     SAARAHYFETIAVLWAAVQGGSEAPTEARGRLRLACAHAVEVSADVCKVAYDMMGGAA
                     VYLENDLQRRFRDAHVITHHAMVAPAVYELAGRVLLGLPTRADQL"
     assembly_gap    4789..4887
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4888..>5182)
                     /locus_tag="PLO65_09210"
     CDS             complement(<4888..>5182)
                     /locus_tag="PLO65_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28465.1"
                     /translation="PASLGGHDTPPAQIALALETLAQADASAAWCLMIGATTGALANR
                     LPAGVAREVFGDPGVIAAGVFAPMGQAVDDGDCWKVTGRWQWGSGSQNANWIAG"
BASE COUNT          742 a         1711 c         1836 g          794 t
ORIGIN      
        1 atcgcaaggt caaacgccgc gcgcgctttc gccttcgcgt ccggccagtc ggcctgcgcg
       61 gccagttcgg cccacaggtc gcgcccgccg accacgaagt gtcgccagcg tgcgtggaag
      121 gggaccgcga gggtcggata ctggtcgcga atgaccgagg ccacgacgtc cgccgtgtgc
      181 ggcaggcggt cgaggttcac ccgccagtcc gtcaggcccc cgtccagggc gatggccagc
      241 atctcatgcg cccgctcccg cacggcggcc gcggagagga gggaacgggc ggcggcggcg
      301 tcagtcatgg gaacacccgg ggacatgtac cgaaggtacg tgtcccctaa tcgagcagaa
      361 ccgtttgggg gacacgtacc ttcggtacat gtccccggag gcctcagaac tccccaagat
      421 cgcgtcccac ggtcttcttc agatcttccg ccgtcggcgc gccgtcgggg gtgaagtagc
      481 cggcggcctt cttggcttcc atctcgacgc tggcgtcgtc gggcaccagc caggccggga
      541 tcggcacgcg ttcgccgatg gtgatgccgc cctcgaccag ggcgtcgtgc ttcatgttgg
      601 acatcgagac gaagcggtcg atgtgcttga tccccagcca gtgcagaacg tcgggcatca
      661 gctgctggaa gcgggcgtcc tggaccccgg ccacgcactc ggtgcgctcg aagtaggtgg
      721 ccgcctggtc gccgccgggc tggcgcttgc gggcgttgta gacgaggaac ttggtcacct
      781 cgcccagcgc ccggccttcc ttgcgattgt agacgatgag gccggcgccg ccgttctggg
      841 cctcgagcac ggcctcctcg atgccgtgga tcaggtaggg gcggcaggtg cagatgtcgg
      901 agccgaacac gtcgctgccg ttgcactcgt catgcacgcg gcaggtcagg cggcgggccg
      961 ggtcggcgat cgcggccggg tcgccgaaga tatacagggt gatgccgccg atcggaggca
     1021 ggaacacctt gatgtccggc cgggtgacca gttcggggta catgccgccg gtctgttcga
     1081 acagggtgcg gcgcagggtg gtctcgtcga cctcgaaccg ctcggcgatg ccgggcagat
     1141 accagaccgg gtcgagggcc gccttggtca cggccacgtc gcgggtttcg tggaccacgg
     1201 tcccgtcgat cctgagccgg cccgcgtcga tcgcctcgcc gatctcgggc agggtcagcc
     1261 gggccttggt gatggcgatg gacggccgga tgtcgacgcc ctcggccacc tcggcggcga
     1321 agtcctgctg gatgcggtga ccccaggggt cgagcgagac gatccggtgc gggtcggccc
     1381 actgcgggaa cgggccgaca tcgaccaccg gcgcggtgtc ggtcaggtcg ggccggacca
     1441 gcgggttcag cgcgcccgag gcgatcgcca ggctgcgata gatggaatag gcgccgccat
     1501 gggcgccgat ggcgttgcgg tcgttgccgg cgttgacggt ggcgacgacc gggccccgct
     1561 cgcgagcggt cggggcgccc caggccaggg ggaaacggcc gggcgagcct gaccccgggt
     1621 gggaggtcag gcggatgtgg ccttgacggt ttacggacat attcagcttg cgcgcggtcg
     1681 gcgcctccag actcgaaaag gcccctcccg gttttcgccg gcgaggagcc tttgagcgag
     1741 gatagtgctc cggggatttc gacttggcaa gccggtgcga gtgcgaggcg attactcgcc
     1801 gtcggcgcgc tgaaagcgga acagcacggt atcgccctcc accacctcga cggcgaggtg
     1861 atgcggcgac tcttcgagct tctggcgcgc gatatcggcg acccgctcct cggccgccgc
     1921 ctcgacgagc aacagcgacg gaaccgaata ccggacgtcg cgaatataga tggcgtaggt
     1981 ccgcacgccc cgaccccctg tcgcgatccc ggatcacgct aacacggccg gccgcgctcg
     2041 tttgtcggct cgccgacgcc gtagccgcct tgacccggaa ggccggccgc cgtgagatgg
     2101 cggccatgac cgatacgatc gaagacttca ttcgcggcct gccgaaggcc gaactgcacc
     2161 tgcatatcga gggcagcctc gagccggagc agatgttcga gttcggccgg cgcaaccgcg
     2221 tcgacatgcc gttcaactcg gtcgaggagg tgcgcgccgc ctacgccttc tccaacctgc
     2281 aggacttcct cgacatctac taccagggcg cggcggtgct gcggaccgag caggacttcc
     2341 acgacatggc catggcctat ttccgccggc tgcacgccga cggcggggtg catgcggaaa
     2401 tcttcttcga cccgcagacg cacacggatc gcggcctgcc gttctcggtc gcgatcgagg
     2461 gcctgctgtc ggggatgaag gcggcggagg cggagctggg cgtcacctcg ggcctgatca
     2521 tgtgcttcct gcgccacctc gacgaggact cggcgatggc gacgctgaag gcttccgagc
     2581 cctggctgga ccggcttgtg ggagtcggtc tggattcctc cgaggtcggc catccgccgt
     2641 cgaagttcca gcgggtgttc agggcggcga aggagcgcgg cctgacgctg agcgcccacg
     2701 ccggcgagga gggtccgccg gcgtacgtgt gggaagcgct ggacctgctc aaggtcgacc
     2761 gcatcgacca cggcaaccgg gcgctggagg acccggctct ggtgcagcgg ctggtggccg
     2821 gggagatgac cctgacggtc tgcccgctgt cgaacctgaa gctctgtgtc gtgaacgacc
     2881 tgacggcaca tccgatgaaa cggatgctgg acctggggtt gaaggccacc tgcaacagcg
     2941 acgaccccgc ctatttcggc ggctacatcg gcgagaattt cattcagacc gcgcgggcgc
     3001 tggacctctc gcgggacgag atcgtgacgc tggcgaagaa cagcttcacc ggctcgttcc
     3061 tcgacgagga gcgggtcgcg catcatctgg cgtcgatcga cgcccatgcg gcgtagggga
     3121 catgtaccca aggtccatgt cccccaatcg ggcgcgcggt tttggggaca cgtaccttcc
     3181 gcacatgtcc cctcaaccta ccgggtgatg cggtagcggc cacggctgtc gggacagacg
     3241 cggacgtagc ggtattccac ccggccggcg tattcggccg gggccggagc cagacggcaa
     3301 ccgcggcgct cgtagtagct gtactcgtag cggccgctgc gggcgcggcg atcccggtag
     3361 tagcgcgcct cgcggtaggg aatggtctcg tcccggctgt accagtagcc gcgatcgtcg
     3421 cagcgggcgc tgtcgcggcc gatgctggcg ccgaggccgc cgccgaccac ggcgccgagc
     3481 accgcgcctt cggtctgcac gtcggtcgcc gccacgttcg agccgatcgc cgccccggcc
     3541 agggcgccga tgatcaggcc cgcggcggtg ttgccgctgc gacgctgttc gcagggactg
     3601 ttgcgataac ggtcgacgtc gacccgcgcc gagacgtcgc ggccgtagta gccggcgtcg
     3661 tagggatagc ggctgtcgta gggctggtag gcgtaggggc ccggatccca gcgccaggcg
     3721 ccgtagcgcc gttcgtaggc gcccacgcca tagcggcggt cgtaggcggc gcggtcgcgc
     3781 aggtactggg cccgcttggt ctcataggcc cgccgctggt cggcataggc ctgctggcgg
     3841 tcctggtagt cttcccgctg ctgctggtag gcgccctgct gctgctcgta ggcgcgctgc
     3901 tgggcctcgt attcctccca ggtcggcacg ttctgggcga tggcggcgcc cgcgctcagg
     3961 gccagcacgc tggtcagggt aaggacgatg gttctggtct tcatgtcgtc gctccgtttc
     4021 ctctcaaggc ggagcgaaca cccgggctgg tgaccggccg acgctctcgc gagcgcccac
     4081 ccccgatacg cctgactgaa cgcgggtcgc gttaaccggt tgcgttggga caggggacat
     4141 atgccgacgc cggtacgtgt ccccccggcc taaagctggt cggcccgggt cggcaggccc
     4201 agcagcaccc ttccggccag ctcatagacg gcgggcgcga ccatggcgtg gtgggtgatg
     4261 acgtgggcgt cgcgaaagcg gcgctgcagg tcgttctcga gatagacggc cgcgccgccc
     4321 atcatgtcgt atgcgacctt gcagacatcg gcgctgacct cgacggcatg ggcgcaggca
     4381 aggcgcagcc ggccgcgagc ctcggtgggc gcctccgacc ctccctggac ggccgcccac
     4441 aacacggcga tggtctcgaa gtagtgggcc cgggcggccg acagccgggc cacggccctg
     4501 gcgaagtcgg cctgcaccac gttgcgctcg gccagcgcgc gttgcgagcc cgaggccttc
     4561 ttcgtctggg ccgccgagcc ggcggtcagc agggccgccc gggcgttgcc cagggccacc
     4621 gcggtgacgc ccagcgccag catgccgaac accgggaaac ggcccagcgg cgtgttgacc
     4681 gtcggtggat cggactgcag cgccacgctg cgcgccttgg gcacgcgcac gtccttgacc
     4741 tggaagtcga gactgcccgt tccgcacagg cccgccgtgc gccaggtgnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc ggcgatccag ttggcattct gcgagccgct
     4921 gccccattgc cagcggccgg tgaccttcca gcaatcgccg tcgtcgaccg cctggcccat
     4981 cggggcgaac acgccggcgg cgatcacgcc gggatcgccg aacacctcgc gcgccacgcc
     5041 ggccggcagg cggttggcca gggcccctgt ggtggcgccg atcatcaggc accaggcggc
     5101 cgaggcgtcg gcctgggcca gggtctccag ggccagcgcg atctgcgccg gcggcgtgtc
     5161 gtggccgccg aggctggccg gc
//
LOCUS       DAORNX010000188         5171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11914_length_5171_cov_7.780102, whole genome shotgun sequence.
ACCESSION   DAORNX010000188 DAORNX010000000
VERSION     DAORNX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11914_length_5171_cov_7.780102"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="PLO65_09215"
     CDS             <1..96
                     /locus_tag="PLO65_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28466.1"
                     /translation="SKDRIEQAKADWRAGRMKLPDLDDGEFIPLP"
     gene            190..351
                     /locus_tag="PLO65_09220"
     CDS             190..351
                     /locus_tag="PLO65_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28467.1"
                     /translation="MVLQGLGPCLIVSLLRASREREPDSDWLLYGPADPEVVEAPAVE
                     APALLLAAE"
     gene            complement(361..993)
                     /gene="pgsA"
                     /locus_tag="PLO65_09225"
     CDS             complement(361..993)
                     /gene="pgsA"
                     /locus_tag="PLO65_09225"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRD28468.1"
                     /translation="MKHLPNLITGLRLVLTLVVFLAMLGLSSQPFVMWAVGKGLDPQL
                     VQTGLYFTAFWGFAVAALTDFLDGWLARRLQIESTLGAILDPIADKVLLAGAVVGLAT
                     LGSWMVACAGGLMLFREFAVSALRETLAPRGLKLPVTLLAKWKTTLQLLALGGELFVG
                     GWIAWGLPRDPALAQKASMAADILLWLAVAVTLWTGAEYARAARKALKAA"
     gene            complement(1075..2946)
                     /gene="uvrC"
                     /locus_tag="PLO65_09230"
     CDS             complement(1075..2946)
                     /gene="uvrC"
                     /locus_tag="PLO65_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRD28469.1"
                     /translation="MSLRVTSASDIPLTGAALIADHARRLPDAPGVYRMIGEDGEVLY
                     VGKARSLKKRVIQYAQGRFHTNRIAHMVDLTRAMEFVSTRTETDALLLEINLIKQMKP
                     RFNVLLRDDKSFPEIVIRRDHAAPQLRKHRGAHTIKGDYFGPFASAGAVNRTLNTLQK
                     AFLLRSCSDSVYESRTRPCMLHQIRRCAAPCTGLIALDDYAKLVEESEAFLRGRSRAV
                     MQRLSEQMQAASDDLEFERAARLRDRIRALSSVAQETQVNPETVEEADVFALFAEGGQ
                     ACVQVFFFRAGQNWGNRAYFPRVDKSDSDAEVMSAFIGQFYDDRPLPRSILVNVQPFE
                     HALLGEAFSMKAGRRVEIARPQRGEKKQLVDQAVNNAREALGRRMAESSAQGKLLAGV
                     AEAFGLEAPPERIEVYDNSHIQGTNAVGGMIVAGPEGFRKNQYRKFNIKGTELTPGDD
                     YGMMKEVLRRRFARLVKEEEEGDDSARPDLVLIDGGKGQLDAVLEVMADLGVDDIPVV
                     GVAKGPDRDAGLERMFMPGKTPFMLEPKSPVLYYLQRLRDEAHRFAIGTHRTKRSMEM
                     KRNPLDEIDGVGPGRKRALLHAFGSAREVGKATVGDLMKVDGVSEALAQRIHDHFRK"
     gene            complement(3149..3943)
                     /locus_tag="PLO65_09235"
     CDS             complement(3149..3943)
                     /locus_tag="PLO65_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD28470.1"
                     /translation="MTSAPFETLLTDTPAEGVLLVTLNRPTAGNSLSSQMAEELLALW
                     RDLADPGATRVVILTGAGEKIFCAGADLKERDGMTDAQWQAQHVLFETMRDALVALPV
                     PVIAAVNGAAYGGGFEIALNCDFIHAADTARFALPEVKLGIMPGLGGVQNLVRAAGER
                     RAREILLTGAPFTAAQGLAWGVVNAVWNPAGLIPEAVDLAKAIAANAPLSVRNIRRVA
                     RETAVLSGAEAVAVELAAYNELTPTEDRREGVAAWGQKRKADWKGR"
     gene            complement(4320..4958)
                     /gene="upp"
                     /locus_tag="PLO65_09240"
     CDS             complement(4320..4958)
                     /gene="upp"
                     /locus_tag="PLO65_09240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRD28471.1"
                     /translation="MRKFSGVTVVDHPLVQHKLSRMRDRATSTKTFRELMREISTLLC
                     YEVTRDLALTERAVETPLETTTAPEIAGKKLVFAPILRAGEGMLEGMLTLVPSARVAH
                     IGLYRDPGTRAAVEYYLKVPETLAERQVIVMNPLLATGVTARAAVDRLKEEGARDLRF
                     VCILTCPDGIEALRGDHPDVPIWTAGVDRGLDDRGFILPGLGDAGDRLYGTR"
     gene            complement(4958..>5171)
                     /locus_tag="PLO65_09245"
     CDS             complement(4958..>5171)
                     /locus_tag="PLO65_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HRD28472.1"
                     /translation="PLVVGWRAMTVALLDRIAPLVRERLGVAEADFPLARVLEGGTWA
                     AGRVLAKDRREGGGPPLTITSDGTVF"
BASE COUNT          750 a         1760 c         1871 g          790 t
ORIGIN      
        1 tcgaaagacc gcatcgagca ggccaaggcc gactggcggg ccggccggat gaagctgccg
       61 gacctggacg acggggagtt cattccgctg ccctaggaac gcctcccatc ccctcatgcg
      121 gagggaggga cttgctaccg ccgcacttgc gagtcattct caattgagtg tgcaatcgtg
      181 gaggcgagga tggttctgca aggcttgggc ccctgcctga tcgtcagcct cctgcgggct
      241 tcgagggagc gggagcctga cagcgactgg ctgctctacg ggccggccga tcccgaagtc
      301 gtcgaggcgc ccgcggtcga ggcgccggcc ctgctactgg cggcggagta gtcccgggac
      361 ctacgccgcc ttcagcgcct tccgcgcggc ccgggcatac tccgcgccgg tccacagggt
      421 gacggccacg gccagccaga gcaggatgtc ggcggccatc gaggccttct gggccagggc
      481 cggatcgcgg ggcaggcccc aggcgatcca gccgccgacg aacagctcgc cgcccagggc
      541 cagcagctgc agggtggtct tccacttggc cagcagcgtc accggcagct tgaggccccg
      601 cggcgccagg gtctcgcgca gggcgctgac agcgaactcg cggaacagca taagcccgcc
      661 ggcgcaggcc accatccacg agcccagggt ggccagcccg accacggcgc cggccagcag
      721 caccttgtcg gcgatcggat cgaggatcgc cccgagcgtg ctctcgatct gcagcctgcg
      781 cgccagccag ccgtcgagga aatcggtcag ggcggcgacg gcgaagcccc agaaggcggt
      841 gaagtagagg ccggtctgga ccagctgggg atcaaggccc ttgccgaccg cccacatcac
      901 gaacggctgc gaggacaggc ccagcatggc caggaagacc accagggtca ggaccagccg
      961 caggccggtg atcaggttgg gaaggtgttt catagggctt tggtagaggg ttttggcggc
     1021 ggggccaaat cccgtggcca atcctccccg gcttcgcgcg gagggttgcc acggtcactt
     1081 ccggaagtgg tcgtggatcc gttgcgccag ggcctcgctg acgccgtcga ccttcatcag
     1141 gtcgccgacc gtggccttgc cgacctcgcg ggccgagccg aaggcgtgca gcagcgcgcg
     1201 cttgcggccg gggccgacgc cgtcgatttc gtcgagcggg ttcctcttca tctccatcga
     1261 ccgcttcgtg cggtgtgtgc cgatggcgaa acggtgggcc tcgtcgcgca gccgctgcag
     1321 ataatacagc accggacttt tcggctcgag catgaagggc gtcttgcccg gcatgaacat
     1381 ccgctcgagc cccgcgtcgc ggtcggggcc cttggcgacg ccgaccaccg ggatgtcgtc
     1441 gacgccgagg tcggccatca cctcaagcac cgcgtccagc tggcccttgc cgccgtcgat
     1501 cagcaccagg tcggggcggg cgctgtcgtc gccctcctct tcctccttga ccaggcgggc
     1561 gaagcggcgg cgcagcacct ccttcatcat gccgtagtcg tcgccggggg tcagctcggt
     1621 ccccttgatg ttgaacttgc gatactggtt cttgcggaag ccctcgggcc cggcgacgat
     1681 catcccgccg accgcgttgg tcccctggat gtggctgttg tcatagacct cgatccgctc
     1741 gggcggggct tccagcccga aggcctcggc caccccggcc agcagcttgc cctgcgccga
     1801 gctctcggcc atccgccggc cgagcgcctc gcgcgcgttg ttgaccgcct ggtcgaccag
     1861 ctgcttcttc tcgccgcgct gcggtcgggc gatctcgacc cggcggccgg ccttcatcga
     1921 gaaggcctcg ccaagcagcg cgtgctcgaa tggctggaca ttgaccagga tcgaccgcgg
     1981 cagcggccgg tcgtcgtaga actggccgat gaaggcgctc atgacctccg cgtcgctgtc
     2041 ggacttgtcg acgcgcggga agtaggcgcg gttgccccag ttctggccgg cgcggaagaa
     2101 gaacacctgc acgcaggcct gaccgccctc ggcgaacagg gcgaagacgt cggcctcctc
     2161 gaccgtctcg ggattgacct gggtttcctg ggccaccgac gacagggcgc ggatgcggtc
     2221 gcgcaggcgg gccgcgcgct cgaactccag gtcgtcggag gcggcctgca tctgctccga
     2281 caggcgctgc atcaccgccc ggctcctgcc gcgcaggaag gcctcggact cctcgaccag
     2341 cttcgcgtag tcgtcgagcg cgatcaggcc cgtgcagggc gcggcgcagc ggcggatctg
     2401 gtgcagcatg cagggccggg tgcggctctc gtagacgctg tcggaacagg accgcagcag
     2461 gaaggccttc tgcagggtgt tgagcgtgcg gttgaccgcc ccggccgagg cgaacggccc
     2521 gaagtagtcg cccttgatgg tgtgcgcgcc acgatgcttg cgcagttgcg gcgcggcgtg
     2581 atcgcggcgg atgacgatct cggggaagct cttgtcgtcg cgcagcagca cgttgaagcg
     2641 cggcttcatc tgcttgatca ggttgatctc gagcagcagg gcgtcggtct cggtgcgggt
     2701 cgagacgaac tccatggccc gggtcaggtc gaccatgtgg gcgatgcggt tggtgtggaa
     2761 ccgcccctgg gcgtactgga tcacccgctt cttcagcgag cgcgccttgc cgacatagag
     2821 cacctccccg tcctcgccga tcatccggta gacgccaggc gcgtcgggca gacgccgagc
     2881 gtggtcggcg atcagggcgg cgccggtgag ggggatgtcg gaggcggagg tcacgcggag
     2941 agatataggg gaatcgggcg gggggagtga gggggtgttg gcagcaagct ctatcctctc
     3001 ccgaagggag agggggacca tccggaggat ggtggagagg aagaggctgg tcgtggcggg
     3061 tgatgcccgc ggccctcagc cgcctgctcc tccctctccg accctgcgct gccgggccac
     3121 ctctccctcc gggagaggaa agacgcgctc accgcccctt ccagtccgcc ttccgcttct
     3181 gcccccaggc cgccacgccc tcgcgacggt cctcggtcgg ggtgagttcg ttgtaggcgg
     3241 ccagttccac cgccaccgcc tccgcgccgg acagcacggc cgtctcccgc gcgacccggc
     3301 ggatgttgcg caccgacagc ggggcgttgg cggcgatggc cttggccagg tcgacggctt
     3361 cggggatcag gccggcgggg ttccagacgg cgttgaccac gccccacgcc aggccctggg
     3421 ccgccgtgaa cggcgcgccg gtcagcagga tttcgcgggc gcggcgttcg ccggcggcgc
     3481 ggaccaggtt ctgcacgcca ccgagcccgg gcatgatgcc gagcttgacc tcgggcaggg
     3541 cgaaccgcgc cgtgtcggcg gcatggatga agtcgcagtt gagggcgatc tcgaagccgc
     3601 cgccgtaggc cgcgccgttg accgccgcga tgaccgggac cggcagggcg accagggcgt
     3661 cgcgcatggt ctcgaacagg acgtgctggg cctgccattg cgcgtcggtc atgccgtcgc
     3721 gctccttcag gtccgcgccg gcgcagaaga tcttctcgcc cgcgcccgtg aggatgacga
     3781 cgcgggtggc gccggggtcg gcgagatcgc gccacagggc gagcagttcc tccgccatct
     3841 ggctggacag gctgttgccg gccgtggggc ggttgagggt gacgagcagg acgccctcgg
     3901 cgggggtgtc ggtcagcagg gtctcgaagg gggcggaggt catggggaag ggatagcccc
     3961 cgggttccat tcaggccaga tgacaatagc cctttggcct tgatggaatt gtccgctccg
     4021 ggcgccggcc ttcgtcagag accgcttcgc ggccgcgcag cggcggcccg cagggccgtc
     4081 cttgaccaag gtcgtcgccg gagcaagctg gccgtcaaga catcagggcg cggtctgccc
     4141 gcgactttcc tctccctcct gggagtgggg gaccacccgg agtgtggtgg agggggaggc
     4201 cgggtggcgg cggagagaat ccggatttcg cggcgacctg cggcgcatcc ctctccgacc
     4261 cggctccgcc gggccacctc tcccaacagg gagaggaaga aggcagcgct gattcgtcgc
     4321 tatcttgtcc cgtacagccg gtccccggcg tcgcccaggc ccggcaggat gaacccccgg
     4381 tcgtccagcc cgcggtccac gcccgccgtc cagatcggca cgtccgggtg gtcgccgcgc
     4441 agggcctcga tgccgtccgg gcaggtcagg atgcagacga accgcaggtc cctggccccc
     4501 tcctccttca gcctgtcgac cgcggcgcgg gcggtgacgc cggtggccag cagggggttc
     4561 atgacgatga cctggcgctc ggcgagggtc tcgggaacct tgaggtagta ctcgaccgcc
     4621 gcccgggtgc ccggatcgcg gtagagacca atgtgggcca cgcgggccga gggcacgagg
     4681 gtcagcatgc cctcgagcat gccctccccg gcccgcagga tcggggcgaa gaccagcttc
     4741 ttgccggcga tctccggggc ggtggtggtc tccagcggcg tctcgaccgc gcgctcggtc
     4801 agggccaggt cgcgggtcac ctcgtagcag agcagggtcg agatctcgcg catcagttcg
     4861 cggaaggtct tggtcgaggt cgcgcggtcg cgcatgcggc tcagcttgtg ctgcaccagc
     4921 gggtggtcga cgacggtcac cccggagaac ttgcgcatca gaacacggtc ccgtcgctgg
     4981 tgatggtcag gggcggaccg ccgccctcgc gccggtcctt cgcaagaacc cggccagccg
     5041 cccaggtgcc gccctcgagc acgcgggcca gcgggaagtc ggcctcggcc acgccgagcc
     5101 gttcgcgcac cagcggggcg atgcggtcga gcagggccac cgtcatcgcc cgccagccga
     5161 ccaccagcgg g
//
LOCUS       DAORNX010000189         5166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11935_length_5166_cov_9.194874, whole genome shotgun sequence.
ACCESSION   DAORNX010000189 DAORNX010000000
VERSION     DAORNX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_5166_cov_9.194874"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLO65_09250"
     CDS             complement(<1..1228)
                     /locus_tag="PLO65_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD28473.1"
                     /translation="MDLISETVVEGRPGPSAGRAYPVLDGVNLTDIGRFSAGQPWADF
                     ARMRREAPVMWHAEPNGGPGFWAVTRHADVMAVDSDAETFSSQRGGILMAIGKPEQRH
                     SMLFRASMDAMINMDAPRHMQLRREHMPYFTPGYLRGLTDRVKAEVTRLLDAMAAKGE
                     CDLVEDFSAVLPLFTLCEILGVPPEDRPRFLKWMHYLEMAQNLATQQAAADMAPTLEL
                     MQFIADFNANVEEMFEYGRTMLHKRRLDPQQDLMSAIARAQVDGELLADEFLDGSWLL
                     IVFAGNDTTRNSLSGTMRLLTEFPDQKARLIADPALLPGAVNEFIRMVSPVIYMRRTA
                     TRDTQIAGQAIAEGEKVIMYYGAANRDESLFPDPDRLDVARANADRHIAFGYGPHVCI
                     GKRVAQIQLEEAYRQIL"
     gene            1414..3084
                     /locus_tag="PLO65_09255"
     CDS             1414..3084
                     /locus_tag="PLO65_09255"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HRD28474.1"
                     /translation="MSETIEADYIIIGAGSAGCVLANRLSADGKTRVLLLEAGGDDRP
                     TKNPGQFFSNLMIHVPVGYSQTLKDPKVNWLYSTEPDPGTGDRVHVWPRGKVLGGSSS
                     INGLLYIRGQDADYDNWRQLGCEGWSWDDVLPYFLRSQHQERDAGPLHGKGGPLNVSD
                     VTTTHPVSDAVIDACEQAGIPRRADLNGGDQEGVSYYQLTVKNGQRCSAAVAYLHPVM
                     GRPNLRVETNALASKVLFEGRRAVGVAFTQNGVKRRAKAAKEVILSGGAVNSPQLLQL
                     SGVGPGALLREHGIEVVADRPGVGENLQDHYVMTQTYRLKPEVVSVNELTKGARFVGE
                     AMKFLFQRKGLLTLSAAHIAVFCKSRPDLAHPDTQFHILPATMDTEKLMLEQKMELEG
                     QPGLTIAPCQLRPESRGHIRIKSADPSAYPAIVANYLSDPLDQEVAVASLKWGRRIAG
                     QPALAPYLAHEMMPGPDIQSDAALLEYARAAGTTIYHPVGTCQMGAGPQAVVDAQLRV
                     HGVEGLRVVDASIMPRLVSGNTNAPTIMIAEKAADMILGKAAPSALAA"
     gene            3124..4665
                     /locus_tag="PLO65_09260"
     CDS             3124..4665
                     /locus_tag="PLO65_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRD28475.1"
                     /translation="MHPIHHARNTPDKVAYIMAGSGESVTYRQLDERANQGAHLFRSL
                     GLKRGDSVALFMDNSPRYYEILFATERAGLYITCISSKLTAGEIEYIVGDCGAKVLIT
                     GRSLAPVAVEAAARLPGVKLYMCEGVVEPFESFEEARAAFPTTMIDDPSPGMTMLYSS
                     GTTGRPKGVRRPLPEGEVGAAPGPLMMLLVGLYGSSPDSVYLSPAPLYHAAPLGWSAG
                     MLQLGGTVVVMEKFDPEQALKYIEQYKVTVAQWVPTHFVRMLKLPEDVRMKYDVSSLQ
                     AVFHAAAPCPVPVKEQMIGWWGPIIHEYYAGTEGNGLTAINSEEWLTHKGSVGKGLTA
                     EVKICDEDGNPLPPRQEGGVYFAGGGVYEYHNDPKKTAEAMNKHGWTTLGDVGWLDEE
                     GFLYLTDRKSFMIITGGVNVYPQEIENLLVTHPKVADVAVVGAPDEEMGEKVVAVVQP
                     ASWADAGDDLAAELNEFVRANLSGVKRPRRIDFMEQLPRHDTGKIYKRLIRDAYWADK
                     TAKIG"
     gene            4709..>5166
                     /locus_tag="PLO65_09265"
     CDS             4709..>5166
                     /locus_tag="PLO65_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HRD28476.1"
                     /translation="MKSIVRIGGAGGFLGDSSVAAPQLLRGGNLDYMILDYLAEATMS
                     SLGQLRAARPDQGYARDFTEWVWKDNLRALKAQGVKIVTNAGGLNPRACRARMEELAA
                     AAGLSFRIAVVEGDDLAGRLGELAGDGLTEMFTGAPFPDPAKVFSANAYFG"
BASE COUNT          858 a         1772 c         1769 g          767 t
ORIGIN      
        1 cgaggatctg gcggtaggcc tcctccagct ggatctgcgc cacgcgcttg ccgatgcaga
       61 cgtgggggcc gtagccgaag gcgatgtgcc ggtcggcgtt ggcccgcgcg acgtcgagcc
      121 ggtcggggtc ggggaacagg ctctcgtcgc ggttggcggc gccatagtac atgatcacct
      181 tctcgccctc ggcgatggcc tggccggcga tctgcgtgtc ccgggtggcc gtgcggcgca
      241 tgtagatcac cgggctgacc atgcggatga actcgttgac cgcgccgggc agcagggccg
      301 gatcggcgat cagccgcgcc ttctggtccg ggaactcggt caacagccgc atcgtgcccg
      361 acagcgagtt gcgggtcgtg tcgttgccgg cgaagacgat cagcagccac gaaccgtcga
      421 ggaactcgtc ggccagcagc tcgccgtcga cctgggcccg ggcgatggcg ctcatcaggt
      481 cctgctgggg gtcgagtcgg cgcttgtgca gcatcgtgcg gccatactcg aacatctctt
      541 ccacgttggc gttgaagtcg gcgatgaact gcatcagctc cagtgtcggc gccatgtccg
      601 cggcggcctg ctgcgtggcc aggttctggg ccatctcgag atagtgcatc cacttgagga
      661 accgcggccg gtcttccggc ggcacgccga ggatctcgca cagggtgaac agcggcagga
      721 cggccgagaa gtcctcgacc aggtcgcatt cgcccttggc ggccatggcg tcgagcaggc
      781 gcgtgacctc ggccttcacc cggtcggtca gaccgcgcag gtagcccggc gtgaaatacg
      841 gcatgtgctc gcggcgcagc tgcatgtggc gcggcgcgtc catgttgatc atcgcgtcca
      901 tcgaggcgcg gaacagcatg gagtggcgct gctcgggctt gccgatggcc atcaggatgc
      961 cgccgcgctg cgacgagaag gtctcggcgt cggagtcgac ggccatcacg tcggcgtggc
     1021 gcgtgaccgc ccagaagccg ggtccgccgt tgggctcggc gtgccacatc accggcgcct
     1081 ctcgacgcat gcgggcgaag tcggcccacg gctggcccgc cgaaaaacgg ccgatgtcgg
     1141 tcaggttcac gccatcgagg accggatagg cgcggcccgc ggagggaccg ggccgcccct
     1201 cgaccaccgt ttcgctgatc aggtccatgc cgcgtgctcc cgcattgtcg ctgtcggggc
     1261 gaccataggc gagcgccttc gcggggcaat cgtcacgtcg cgtcagcttc cgccacggcg
     1321 ccctttccag atggcctgcc gcgcacgtaa agtgatcacc gatcaggcgc gcgccagacg
     1381 cgccggtcgt cgaggacagc agggagcggc cgcgtgagcg agaccatcga ggcggactac
     1441 atcatcatcg gcgccggcag cgccggctgc gtgctggcca accggctcag cgccgatggc
     1501 aagaccaggg tcctgctgct cgaggccggc ggcgacgacc ggccgacgaa gaacccgggc
     1561 cagttcttct ccaacctgat gatccatgtg ccggtcggtt actcgcagac gctcaaggac
     1621 cccaaggtca actggctgta ttccaccgag ccggatcccg gcaccggcga tcgcgtgcac
     1681 gtctggccac gcggcaaggt gctcggcggc tcctcctcga tcaacggcct gctctacatc
     1741 cgcggacagg acgccgatta tgacaactgg cgccagctgg gctgcgaagg ctggagctgg
     1801 gacgacgtcc tgccctactt cctccgctcg cagcaccagg agcgcgacgc cggtcccttg
     1861 cacggcaagg gggggccact gaatgtctcg gatgtcacca ccacccatcc ggtgtccgac
     1921 gccgtcatcg acgcctgcga acaggccggc attccccgcc gcgcggacct gaacgggggc
     1981 gaccaggaag gcgtgagcta ctaccagctg acggtgaaga acgggcagcg ctgctcggcc
     2041 gccgtggcct accttcatcc ggtcatgggc cgcccgaacc tgcgggtcga gaccaacgcc
     2101 ctggcctcga aggtgctgtt cgagggcagg cgcgcggtcg gcgtcgcctt cacccagaac
     2161 ggggtcaagc gcagggcgaa ggccgcgaag gaggtcatcc tgtcgggcgg cgcggtcaac
     2221 tcgccgcagc tgctgcagct gtcgggcgtc ggcccgggcg ccctgttgcg cgaacacggc
     2281 atcgaggtgg tcgccgaccg gccgggcgtc ggcgagaacc tgcaggacca ctacgtcatg
     2341 acccagacct accgcctcaa gccggaggtg gtgtcggtca acgaactcac caagggcgcc
     2401 cgcttcgtcg gcgaggcgat gaagttcctg ttccagcgca agggtctgct gaccctgtcg
     2461 gcggcccata tcgcggtgtt ctgcaagtcc cggccggacc tggcgcaccc cgacacccag
     2521 ttccacatcc tgccggcgac catggacacc gaaaagctga tgctggagca gaagatggag
     2581 ctggagggcc agcccggcct gaccatcgcc ccctgccagc tgcggcctga aagccgcggc
     2641 cacatccgca tcaagtcggc cgacccgtcg gcctatccgg ccatcgtcgc caactatctc
     2701 agcgatccgc tggaccagga ggtggccgtc gcgtcgctga aatggggccg caggatcgcc
     2761 ggacagccgg cgctggcgcc ctatctcgcc cacgagatga tgccggggcc ggacatccag
     2821 tccgatgcgg cgttgctgga atacgcccgg gcggccggca cgacgatcta tcaccccgtc
     2881 ggcacctgcc agatgggcgc cggtccgcag gcggtggtcg acgcccagct ccgggtccac
     2941 ggggtcgagg gcctgcgggt cgtcgacgcc tcgatcatgc cccggctggt cagcggcaac
     3001 accaacgccc cgacgatcat gatcgccgaa aaggccgccg acatgatcct cggcaaggcc
     3061 gcgccgtcgg ccctcgccgc ctgacacctg ccgtttcaac cccagtcgaa cagtctgagc
     3121 cccatgcacc cgatccacca cgctcgaaac acgcccgaca aggtcgccta catcatggcc
     3181 gggtcgggag aatcggtgac ctaccgccag ctcgacgaac gggccaacca gggcgcccac
     3241 ctgttccgct cgctggggct gaagcggggc gactcggtcg ccctcttcat ggacaacagc
     3301 ccgcgctact acgagatcct gttcgccaca gagcgggccg gactctacat cacctgcatc
     3361 tcgtccaagc tgacggccgg cgagatcgag tacatcgtcg gcgactgcgg cgcgaaggtg
     3421 ctgatcaccg gccggagcct ggcccccgtg gccgtcgagg ccgccgcccg gctgcccggc
     3481 gtaaagctgt acatgtgcga gggcgtggtc gagccgttcg agagcttcga ggaggcccgc
     3541 gcggccttcc ccacgacgat gatcgacgat ccgtcgccgg gcatgaccat gctgtattcg
     3601 tcaggcacca ccggccgtcc caagggcgtg cggcgtcccc tgccggaagg cgaggtcggc
     3661 gccgctcccg gaccgctgat gatgctgctg gtcggcctgt acggctcgag cccggacagc
     3721 gtctacctgt cgcccgcgcc gctgtatcac gccgcgccgc tgggctggtc ggccggcatg
     3781 ctgcagctgg gcggcaccgt cgtggtgatg gagaaattcg acccggagca ggccctgaaa
     3841 tacatcgagc agtacaaggt gacggtcgcc cagtgggtgc cgacgcactt cgtgcgcatg
     3901 ctcaagctgc ccgaggacgt gcgcatgaag tacgacgtct cctcgctgca ggcggtgttc
     3961 cacgccgccg ctccctgccc cgtgccggtc aaggaacaga tgatcggctg gtggggtccg
     4021 atcatccacg agtactacgc cggcaccgag ggcaacggcc tgaccgcgat caactccgag
     4081 gagtggctca cccacaaggg ctcggtcggc aagggcctga ccgccgaggt gaagatctgc
     4141 gacgaggacg gcaaccccct gccgccgcgc caggagggcg gcgtctactt cgccggcggc
     4201 ggggtctacg aataccacaa tgacccgaag aagaccgccg aggccatgaa caagcacggc
     4261 tggaccaccc tgggcgacgt gggctggctg gacgaggagg gcttcctcta cctgaccgac
     4321 cgcaagagct tcatgatcat taccggcggc gtgaacgtct atccgcagga gatcgagaac
     4381 ctgctggtga cccatcccaa ggtggccgac gtcgccgtcg taggggcgcc ggacgaggag
     4441 atgggcgaga aggtggtggc ggtcgttcaa ccggccagct gggccgacgc cggcgacgac
     4501 ctggcggccg agctgaacga gttcgtgcgc gccaacctca gcggcgtgaa acggccccga
     4561 cggatcgact tcatggagca gctgccgcgc catgacacgg gcaagatcta caagcgcctg
     4621 atccgcgacg cctactgggc cgacaagacc gccaagatcg gctagcggcc ccgacgacgg
     4681 cccggagtcc gggcgaggac gtgatgtgat gaagagcatc gtacggatcg gcggcgccgg
     4741 gggcttcctc ggcgactcgt ccgtcgccgc cccgcagctg ctgcgcggcg ggaacctcga
     4801 ctacatgatc ctcgactacc tggccgaggc gacgatgtcg tcgctgggcc agctgcgcgc
     4861 ggcccggcca gaccagggct acgcccgcga cttcaccgaa tgggtctgga aggacaacct
     4921 gcgagcgctg aaggcgcagg gtgtgaagat cgtcaccaac gccggcggac tcaatccgcg
     4981 cgcctgccgc gcaaggatgg aggagctggc cgccgccgcg gggctgtcgt tccgcatcgc
     5041 cgtggtcgag ggcgacgacc tggccggacg gctcggtgag ctggcgggcg acggcctgac
     5101 cgagatgttc accggcgcgc ccttccccga tccggcgaag gtgttcagcg ccaacgccta
     5161 tttcgg
//
LOCUS       DAORNX010000190         5157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11957_length_5157_cov_9.390631, whole genome shotgun sequence.
ACCESSION   DAORNX010000190 DAORNX010000000
VERSION     DAORNX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11957_length_5157_cov_9.390631"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..703)
                     /locus_tag="PLO65_09270"
     CDS             complement(227..703)
                     /locus_tag="PLO65_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HRD28477.1"
                     /translation="MANPELGAKQICPNCQSKFYDLGRRPAACPKCGETFDPEEAVRN
                     RRVRARSSVPDYEDAEEKPVKAVDPDADGFEEEPDQTPEIDDEAAAEPIETDEESDDP
                     VVAPAPADDLGVDFAEDEELADEEADGVPFLEDEDEDDFPEDEIDGLPGEDDAEDR"
     gene            819..2138
                     /gene="aroA"
                     /locus_tag="PLO65_09275"
     CDS             819..2138
                     /gene="aroA"
                     /locus_tag="PLO65_09275"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRD28478.1"
                     /translation="MTAAVLSARPGAPLVGTVRVPGDKSISHRALILGAMASGRTTIE
                     GLLEGEDVLATARAMRAFGATVTRMGEGAWTVEGRGAFSAPAETIDCGNAGTGVRLIM
                     GAAAGYPVTATFTGDASLRKRPMGRVLEPLGQMGARFEARDGGRLPLTLTGGGLGGIV
                     YRLPMASAQVKSAVLLAGLNAGGDVTVIEPEATRDHTERMLRAFGAHVTVRDEADGRR
                     IHLAAGQRLVGTHVVVPGDPSSAAFPVVAALITPGSAVTVENVMLNPLRAGLFDTLIE
                     MGADLTIVNRREAGGELVGDLVARHSALRGVIVPPARAPSMIDEYPVLAVAAAFATGE
                     TVMRGVGEMRVKESDRIALMAAGLEACGVDVEEEPEGFIVRGSGQPPRGGGLVETHGD
                     HRIAMSHLVLGLAAGAAVRVDEPGMIATSFPDFAPMMRRLGGDISEA"
     gene            2142..2792
                     /gene="cmk"
                     /locus_tag="PLO65_09280"
     CDS             2142..2792
                     /gene="cmk"
                     /locus_tag="PLO65_09280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRD28479.1"
                     /translation="MGFTIAVDGPAASGKGTIAVRLGALYGYPVLDSGLLYRAVGMKV
                     LAEGGDLSDEDAATAAARSLEPSELESPIVRTRAAGEAASRVAIYTPVRRVLRDFQRT
                     FAEQEPGAVIDGRDIGTVIAPDAPAKLYVTATPEARAERRWKQLVGQGEAVTYEDILA
                     DIHTRDARDSARADSPMVAAPDAVLLDTTEMTIDAAFDAARRIVEAARSRWETARG"
     gene            2917..4629
                     /gene="rpsA"
                     /locus_tag="PLO65_09285"
     CDS             2917..4629
                     /gene="rpsA"
                     /locus_tag="PLO65_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRD28480.1"
                     /translation="MADDLSLNPSRDDFAALLDASMGGAGDFAEGTVINGKVVGIEKD
                     FAIIDVGLKTEGRILIKEFGVDEDGKATLKVGDTVEVFLERLENALGEAVLSREKAKR
                     EEAWTRLEVVFAKQEPVMGSIVGRVKGGFTVDLGGASAFLPGSQVDIRPVRDVGPLMG
                     KEQPFAILKMDRPRGNIVVSRRAILEEARAEQRTELVSQLQEGEIREGVVKNITDYGA
                     FVDLGGIDGLLHVTDMSWKRVSHPSQVLAVGDTVKVQIIKINPDTQRISLGMKQLQSD
                     PWDGVEAKYPVGAKYTGRITNITDYGAFVELEAGVEGLVHVSEMSWTKKNVHPGKIVS
                     TSQEVDVIVLDVDSSKRRVSLGLKQAMANPWDAFLAAHPIGSTVEGEVKNATEFGLFV
                     GLDGDIDGMVHLSDLDWNASGEEAMAKFKKGDMIKAKVLDVDVEKERISLGVKQLAGD
                     PLVGDTFRKGQTVTVTVTEVTTGGIEVKFGEEDAPMTAFIRKSDLSRDRQEQRPERYA
                     VGDRVDAQVTNIDKAARRVSVSIKALEMAEEKEAIEQFGSSDSGASLGDILGAALREK
                     ASKD"
     gene            complement(4654..>5157)
                     /locus_tag="PLO65_09290"
     CDS             complement(4654..>5157)
                     /locus_tag="PLO65_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28481.1"
                     /translation="LILPLSWTSLRNARARRKLMRLAAAEQVRLRHLVLCEVTGLETG
                     TPQAALREAAGQLQPIFRGVLARATPNRRVIRDLTDCGFTGATVEAAELGDAEDPAAM
                     LRTMLALQKIGPGVLVHAVRSVAGLTAVRAAGASWASLDIVPGALESARLAAIAKTAA
                     GGTPTAA"
BASE COUNT          814 a         1814 c         1782 g          747 t
ORIGIN      
        1 ccgctgacct cttgaatgcc attcaagcgc tctaccagct gagctatagc cccgaggtct
       61 cgggtttccc gcatccggcg ggccggcggc gagggcgcgg ttctaggccg tcatccctcg
      121 ccgatcaaga gccgctgagc ggaatttccg cccggcgtgg ttccggcccg gatgaaatca
      181 tccgaaccgg ttcgcggacc ccgaaacagg gggcccgccg ccggtcctaa cggtcctcgg
      241 cgtcgtcctc gccgggcagg ccgtcgatct cgtcctccgg gaagtcgtcc tcgtcctcat
      301 cctcgaggaa gggcacgccg tcagcctcct cgtcggccag ttcctcgtcc tcggcgaagt
      361 cgacgcccag gtcatcggcg ggggcggggg ccacgacggg gtcgtcggac tcctcgtcgg
      421 tctcgatcgg ttcggccgcg gcctcgtcgt cgatttccgg cgtctgatcg ggctcttcct
      481 cgaagccatc ggcgtcggga tcgacggcct tgaccggctt ttcctcggcg tcctcgtagt
      541 cgggcacgga cgagcgggcg cgcacccgac gattacggac ggcttcttcc ggatcgaagg
      601 tctcgccgca tttcgggcag gcggccgggc ggcggcccag atcgtagaat tttgactgac
      661 agttggggca aatctgtttt gcgcccagtt caggattggc caaggggcga tcctcatagg
      721 tcttgctgcg gaagcggcgc gtcccttgcc acggggcggg ggcgctgtca aaagcaatcc
      781 ccgcccttcc ccaagccttt caggagcttg cgcccggcat gaccgccgcc gtgctttctg
      841 cccgccccgg cgcgcccctt gtcgggaccg tcagagtccc cggcgacaag tcgatttccc
      901 accgggcgtt gatcctcggc gcgatggcca gcgggcgtac gacgatcgag gggctgctcg
      961 agggcgagga tgtgctggca accgccaggg ccatgcgggc cttcggcgcg acggtcaccc
     1021 gcatgggcga aggggcctgg accgtcgagg gccgcggcgc cttttccgcg ccggcggaga
     1081 ccatcgactg cggcaacgcc ggcaccggcg ttcggctgat catgggcgcc gcggccggct
     1141 atccggtcac cgcgaccttc accggggacg cctcgctgag gaagcggccg atgggccggg
     1201 tgctggagcc gctgggccag atgggcgcgc ggttcgaggc ccgcgacggg ggccggctgc
     1261 cgctgaccct gaccggcggc ggactgggcg gaatcgtcta ccggctgccg atggcctcgg
     1321 cccaggtgaa gtcggccgta ctgctcgccg gcctgaacgc cgggggcgac gtcacggtga
     1381 tcgagccgga agcgacccgc gaccacaccg aacggatgct gcgggccttc ggcgcccatg
     1441 tgacggtgcg ggacgaggcg gacggacggc gcattcacct ggcggccggc cagcggctgg
     1501 tcgggaccca tgtggtcgtg cccggcgacc cgtcgtcggc ggcctttccg gtggtggcgg
     1561 cattgatcac gcccggctcg gcggtcacgg tcgagaatgt gatgctcaat ccgctgcgcg
     1621 cgggcctgtt cgacaccctg atcgagatgg gcgccgacct gaccatcgtg aaccggcgcg
     1681 aagcgggcgg cgaactggtc ggcgacctgg tcgcgcgaca ctcggccctg cgcggcgtga
     1741 tcgtgccgcc cgcgagggcc ccgagcatga tcgacgagta tccggtcctc gccgtcgccg
     1801 cggccttcgc caccggcgag acggtgatgc gcggcgtcgg cgagatgcgg gtcaaggaga
     1861 gcgatcgcat cgccctgatg gcggcgggcc ttgaggcctg cggcgtcgat gtcgaggagg
     1921 agccggaggg cttcatcgtc cgcgggtccg gccagccccc gcgcggcggc ggcctcgtcg
     1981 agacccacgg cgaccaccgc atcgccatga gccatctggt gctgggtctg gccgccggcg
     2041 cggcggtgcg ggtcgatgag cccggtatga tcgccaccag ctttcccgac ttcgcgccga
     2101 tgatgcgccg tctgggcggc gacatctcgg aggcctgaac catgggtttc accatcgccg
     2161 tcgacgggcc cgcggcctcg ggcaagggca ccatcgcggt caggctgggc gcgctgtacg
     2221 gctatccggt gctggattcc ggcctgctct atcgcgcggt cgggatgaag gtgctggccg
     2281 agggcggcga cctgtccgac gaagacgccg ccacggccgc ggcccgctcc ctggaaccgt
     2341 ccgagctcga gagtccgatc gtccgcaccc gcgccgccgg cgaggcggcc agccgggtgg
     2401 ccatctacac gccggtgcgc cgggtgctgc gcgacttcca gcggaccttc gccgaacagg
     2461 agcccggcgc ggtgatcgac gggcgcgaca tcggcacggt gatcgccccg gacgccccgg
     2521 ccaaactcta cgtcaccgcc acgcccgagg cccgcgccga gcgtcggtgg aagcaactgg
     2581 tcggccaggg cgaggcggtg acctatgagg acatcctcgc cgacatccac acccgcgacg
     2641 cccgcgattc cgcccgcgcg gattccccga tggtcgccgc gcccgacgcg gtcttgctcg
     2701 acaccaccga aatgactata gacgcggcct tcgatgcggc ccgccgtatc gtcgaggcgg
     2761 cgcgcagccg gtgggaaacc gccagaggct aatccaagcg cgctccactc gaccggccgc
     2821 gggcgctcaa acccacctga aacccatgga ccgcccggac tccgccggca tcccgccgcc
     2881 tgtccgcgat ccgcaaccaa gagactgaaa ccccaaatgg ctgacgatct gagcctgaac
     2941 cccagccgcg atgacttcgc ggccctcctc gacgcctcga tgggcggcgc cggcgacttc
     3001 gccgaaggca ccgtcatcaa cggcaaggtc gttggcatcg agaaggactt cgccatcatc
     3061 gacgtcggtc tgaagaccga aggccgcatc ctgatcaagg aattcggcgt cgacgaggac
     3121 ggcaaggcga ccctgaaggt cggcgacacg gtcgaggtgt tcctcgagcg tctcgagaac
     3181 gccctgggcg aggccgtgct gagccgcgag aaggccaagc gcgaagaagc ctggacccgc
     3241 ctggaagtgg tgttcgccaa gcaggagccg gtcatgggct cgatcgtcgg ccgcgtgaag
     3301 ggcggcttca ccgtcgacct cggcggcgcc agcgccttcc tgccgggctc gcaggtcgac
     3361 atccgcccgg tgcgcgacgt cggcccgctg atgggcaagg agcagccctt cgccatcctc
     3421 aagatggacc gtcctcgcgg caacatcgtc gtctcgcgtc gcgccatcct ggaagaagcc
     3481 cgcgccgagc agcgcaccga gctggtcagc cagctgcaag agggcgagat ccgcgaaggc
     3541 gtggtcaaga acatcaccga ctacggcgcg ttcgtggacc tgggcggcat cgatggcctg
     3601 ctgcacgtca ccgacatgag ctggaagcgc gtctcgcacc ccagccaggt cctggcggtc
     3661 ggcgacaccg tcaaggtgca gatcatcaag atcaacccgg acacgcagcg catcagcctc
     3721 ggcatgaagc agctgcagtc cgatccgtgg gatggcgtcg aagccaagta cccggtcggc
     3781 gccaagtaca ccggccgcat caccaacatc accgactacg gcgcgttcgt tgaactggaa
     3841 gccggcgtcg agggcctggt gcacgtctcg gaaatgtcct ggaccaagaa gaacgtccac
     3901 ccgggcaaga tcgtctccac ctcgcaggaa gtggacgtca tcgtgctcga cgtcgacagc
     3961 tccaagcgcc gcgtctcgct cggcctcaag caggccatgg ccaacccgtg ggatgccttc
     4021 ctggccgccc atccgatcgg ctcgaccgtc gagggcgaag tcaagaacgc caccgaattc
     4081 ggcctgttcg tgggcctcga cggcgacatc gacggcatgg tccacctgtc cgacctcgac
     4141 tggaacgcgt ctggcgaaga agccatggcc aagttcaaga agggcgacat gatcaaggcc
     4201 aaggtgctcg acgtcgacgt cgagaaggag cgcatcagcc tgggcgtgaa gcagctggcc
     4261 ggcgacccgc tggtcggcga caccttccgc aagggccaga ccgtgaccgt caccgtgacc
     4321 gaggtcacca ccggcggcat cgaggtcaag ttcggcgagg aagacgcgcc gatgacggcc
     4381 ttcatccgca agtcggacct gtcgcgcgac cgccaggagc agcgccccga gcgctacgcc
     4441 gtcggcgacc gcgtcgacgc ccaggtgacc aacatcgaca aggccgcccg ccgggtgtcc
     4501 gtctcgatca aggcgctgga aatggccgaa gagaaggaag ccatcgagca gttcggctcc
     4561 tcggattccg gagcctcgct gggcgacatc ctcggcgcgg ccctgcgtga gaaggcctcg
     4621 aaggactaac cttcgacgcc catcggcaag acgtcaggcg gcggtcgggg ttcccccggc
     4681 cgccgttttg gcgattgccg ccagcctggc cgactccagg gccccgggga cgatgtccag
     4741 gctggcccag ctggccccgg ccgcacgcac cgccgtcagc ccggccaccg accgcacggc
     4801 atggacaaga acgccgggcc cgatcttctg cagcgccagc atcgtgcgca gcatggccgc
     4861 cggatcctcg gcatcgccga gctcggcggc ctcgacggtc gcgccggtga agccgcagtc
     4921 ggtcaggtcc cggatcaccc ggcggttggg cgtggcccgc gcgagcacgc cgcgaaagat
     4981 cggctgcagc tgtcccgccg cctcccgcag ggccgcctgc ggcgtccccg tctccagccc
     5041 cgtgacctcg cacaggacca gatggcgcag ccggacctgc tccgccgcgg ccaggcgcat
     5101 cagcttgcgg cgggcccggg cgttgcgcag cgacgtccag gagaggggca ggatgag
//
LOCUS       DAORNX010000191         5153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11969_length_5153_cov_5.886818, whole genome shotgun sequence.
ACCESSION   DAORNX010000191 DAORNX010000000
VERSION     DAORNX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_5153_cov_5.886818"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLO65_09295"
     CDS             <1..665
                     /locus_tag="PLO65_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRD28482.1"
                     /translation="SPPVRNVIVDMPGTGQAAVTLARRGIARSDPRYYAGMVANTVLG
                     GGYSARLNQEIRVKRGLAYGAGSSLSARRIVGGFTASTQTKNESAAEVVTLIRGELAR
                     MATVPATPAELAARKSVLVGDFGRDLGTTEGLAGVLGGLAAYDIDLGELNRFTGKVEA
                     VTADEVSAFAADVFDPSKASTIVVGDVKLFGEAIKTAAPDAQVIPLDQLDLDSPTLAK
                     GP"
     gene            809..1990
                     /locus_tag="PLO65_09300"
     CDS             809..1990
                     /locus_tag="PLO65_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD28483.1"
                     /translation="MTAQIEPFHAIAVSRLAHELKAQGRSIIHMEFGQPSTGAPKAAI
                     AAAHHVLDTDGMGYWESPALRARIARHYRETYGVEVEEGRIILTCGASPALVLALLAA
                     FSPGDRVALARPGYVAYRNTLKALRMEPVEIACGAESRFQLTAAHLAALDPAPAGVII
                     ASPANPTGTIIAADELAAIAEVCRARGIRIVSDEIYHGLSYVEPCRSILEFEPTTLVV
                     NSFSKYFSMVAWRLGWLLAPRDLVASARAYVGNLFLTAPSLSQHAGLAAMDATVELEG
                     HVEVYRRNRALLLEALPQLGLREIAPPDGAFYIYASVAHLTDDSLAFCMDLLRDTGVA
                     VASGVDFDPVEGRRFIRISFAVSTPEVEEALRRLTPWFAARTQGTCTAGTCPPSPSSL
                     D"
     gene            complement(2025..2549)
                     /locus_tag="PLO65_09305"
     CDS             complement(2025..2549)
                     /locus_tag="PLO65_09305"
                     /inference="COORDINATES: protein motif:HMM:NF020909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HRD28484.1"
                     /translation="MTISLYDITVGQYLQIVDAAAGFLERGLKHCADEGANPDDLVGK
                     RLAGDMLPLAFQVSSIAHHSIGAIEAVKSGVFKATGGPAMPETYAGLVKIVADTQTAL
                     RALTPDEVNALAGKDVTFEFGPTKMPFTAEGFLLSFSLPNFYFHASTAYDVLRANGVP
                     LGKRYFLGQLKLKS"
     gene            complement(2634..3548)
                     /locus_tag="PLO65_09310"
     CDS             complement(2634..3548)
                     /locus_tag="PLO65_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD28485.1"
                     /translation="MNLSAVDLNLLPPLEALLRRRNVTRAAADAGLSQPAMSRALGRL
                     RELLDDALLVRQGGGFVLTPFAEGLVPQLAAALEEVQGLFSRPAFDPRAETRTVRLVA
                     SDIHTVLLVPPLMARLAVEAPGVTLRIEGYGPELPLRMTSGEVDLTFAVTGAHLPPGA
                     VSAPIARDSMVVAMRRGHPASGRPLTLAGYARLGHVGVSLFGDGQSMIDSVLAAEGLE
                     RRIALTTPSFVAALATVAAGDGVTAVSRTLAARFVDALDLHLAAAPFPGADYEMTLVW
                     ANHRSGDALLAWLRDQISEIAGEVFAAD"
     gene            3636..4265
                     /locus_tag="PLO65_09315"
     CDS             3636..4265
                     /locus_tag="PLO65_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRD28486.1"
                     /translation="MSVNAASPVAPRMGWPFWIVLITSLLIALASVRYLVPGAPGGAP
                     AIMANAFTRYGVLTVHAGFAMVALTIGPFQFLRGLRTRRPRLHRWMGRTYVLACLVAG
                     AAGLLLAFGATTGPVSTAGFGLLAVLWLYTTTRAWTSARGRRFDEHRRWMIRSFALTF
                     AAVTLRLYLPFAFFSPFGYDDTYRAISFLCWVPNLLFAEWYLRARPQVP"
     gene            complement(4253..4642)
                     /locus_tag="PLO65_09320"
     CDS             complement(4253..4642)
                     /locus_tag="PLO65_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28487.1"
                     /translation="MDWQNTAFIAAGAVGGITAAIHGVLIERLMARPFDTILAGDRRV
                     SLVIRRLVAPLLHYSTISWLLGGLVLIAAAVCPFSPDARLAVGLMVGSHFLYGALGNA
                     WATRGRHPGWMLMALAVGLIAWALYGT"
     gene            4718..>5153
                     /locus_tag="PLO65_09325"
     CDS             4718..>5153
                     /locus_tag="PLO65_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28488.1"
                     /translation="MSDRLTKADWIRHGLLTLARHGPGGLKVGPMATALKVSRGSFYW
                     HFEDIGDFRAQLLQGWREVSTDQIIQDLDGTAAPDRLKRLMRRAFGMRLTLERAVRAW
                     GAEDRNVAAAVAAVDARRVGHIAGLLATSGVDAERAGQRATFL"
BASE COUNT          755 a         1852 c         1794 g          752 t
ORIGIN      
        1 ccagcccgcc ggtgcgcaac gtgatcgtcg acatgcccgg caccggccag gcggccgtca
       61 ccctggcccg tcgcggcatc gcccgcagcg acccgcgcta ctacgccggc atggtcgcca
      121 acacggtgct cggcggcggc tactcggcgc ggctgaacca ggagatccgc gtcaagcgcg
      181 gcctggccta cggcgccggc tccagcctct cggcccggcg catcgtcggc ggcttcaccg
      241 cctcgaccca gaccaagaac gagtcggcgg ccgaggtggt caccctgatc cgtggcgagc
      301 tggcccggat ggcgaccgtc ccggccacgc cggccgagct ggcggcgcgc aagtcggtgc
      361 tggtcggcga cttcgggcgg gacctgggca ccaccgaggg cctggccgga gtgctcggcg
      421 gcctcgcggc ctacgacatc gacctgggcg agctgaaccg tttcaccggc aaggtcgagg
      481 cggtcaccgc cgatgaggtc tcggcgttcg ccgccgacgt cttcgacccg tcgaaggcca
      541 gcaccatcgt ggtcggcgac gtcaagctgt tcggcgaggc gatcaagacg gcggcccccg
      601 acgcccaggt catcccgctg gaccagctgg acctcgacag cccgaccctg gcgaaggggc
      661 cgtagcgctt gcctggttcg acacgcaccc tgcgggtgct gctcaccatg acgtgtgccg
      721 aaatacgtca tcctgagcag gcccgcaggg ccgtgtcgaa ggacgcaccg tgcttgcatc
      781 ggggcgcgtt ccacgctcag acccactcat gaccgcgcag atcgaaccgt tccacgccat
      841 cgccgtcagc cggctggccc atgagctgaa ggcccagggg cggtcgatca tccacatgga
      901 gttcggccag ccctcgacgg gcgcgccgaa ggcggccatc gccgcggccc accatgtgct
      961 cgacaccgac ggcatgggct actgggagag cccggccctg cgcgcccgca tcgcgcgcca
     1021 ctatcgcgag acctacggcg tcgaggtcga ggaaggccgc atcatcctga cctgcggcgc
     1081 ctcgccggcg ctggtgctgg cgctgctggc ggccttctcg cccggcgacc gggtggcgct
     1141 ggcccggccg ggctacgtcg cctaccgcaa caccctcaag gccctgcgca tggagccggt
     1201 ggagatcgcc tgcggggccg agagccgctt ccagctgacc gcggcccacc tcgccgcgct
     1261 cgacccggcg ccggccgggg tgatcatcgc cagcccggcc aacccgaccg gcacgatcat
     1321 cgccgccgac gagctggcgg ccatcgccga ggtctgccgg gcgcgcggaa tccgcatcgt
     1381 ctcggacgag atctatcacg gcctcagcta cgtcgaaccg tgccgctcga tcctcgagtt
     1441 cgagccgacc accctggtgg tcaacagctt cagcaaatac ttcagcatgg tcgcctggcg
     1501 gctgggctgg ctgctggccc cgcgcgacct ggtggccagc gcccgcgcct atgtcggcaa
     1561 cctgttcctg accgccccgt cgctgagcca gcacgcggga ctggccgcca tggacgccac
     1621 cgttgagctg gaaggccacg tcgaggtcta tcgccgcaac cgcgccctgc tgctggaggc
     1681 cctgccgcag ctgggcctgc gcgagatcgc cccgccagac ggggcgttct acatctacgc
     1741 cagcgtcgcc cacctgaccg acgacagcct ggcgttctgc atggaccttt tgcgcgacac
     1801 cggggtggcg gtcgcgtccg gcgtcgactt cgacccggtc gaggggcgca ggttcatccg
     1861 catcagcttc gcggtgtcga cgccggaggt cgaggaggcg ctgcgccgcc tgacgccctg
     1921 gttcgcggcg cggacacagg ggacatgcac cgcaggtacg tgtcccccaa gcccgtcatc
     1981 gctcgattag gggacacgta ccgtcggtac atgtccccgc tccatcagct cttaagcttc
     2041 agctgaccca ggaagtagcg cttgcccagc ggcacgccgt tggcgcgcag cacgtcgtag
     2101 gctgtcgagg cgtggaagta gaagttgggc agggagaagg acagaaggaa gccctcggcg
     2161 gtgaacggca tcttcgtcgg gccgaactcg aaggtcacgt ccttgccggc cagggcgttg
     2221 acctcgtcgg gcgtgagggc ccgcagcgcc gtctgggtgt cggcgacgat cttgaccagg
     2281 ccggcgtagg tctcgggcat ggccgggccg ccggtggcct tgaacacgcc cgacttcacc
     2341 gcctcgatgg cgccgatgga atgatgggcg atcgagctga cctggaacgc cagcggcagc
     2401 atgtcgccgg ccagccgctt gccgaccagg tcgtcggggt tggccccctc gtccgcgcag
     2461 tgcttcaggc cccgctcgag gaagcccgcg gcggcgtcga cgatctgcag atactggccg
     2521 acggtgatgt cgtagagcga aatggtcatg gcggtcgcgc ctccccggta cgttgttttg
     2581 tgaaacggtt ggggagattg cccagcgcgc gggccggcgg caatccccaa ccgtcaatcg
     2641 gccgcgaaga cctctccggc gatctcgctg atctggtccc gcaaccaggc cagcagggcg
     2701 tcgccgctcc ggtgattggc ccagaccagg gtcatctcgt agtccgcgcc gggaaagggc
     2761 gcggccgcca gatgcaggtc gagggcgtcg acgaaccggg ccgccagggt tcgcgacacg
     2821 gcggtgacgc cgtctccggc ggccaccgtc gccagggcgg cgacaaagct cggtgtggtc
     2881 agcgcgatgc ggcgctccag cccctcggcc gcgaggacgg aatcgatcat cgactggccg
     2941 tcgccgaaca ggctgacccc gacgtgaccg agccgggcgt acccggccag ggtcaacggg
     3001 cggccggacg ccggatgccc ccggcgcatg gccacgacca tgctgtcgcg ggcgatcggc
     3061 gcactgaccg cgccgggcgg cagatgggcg ccggtgaccg cgaaggtcag gtcgacctcg
     3121 ccgctcgtca tccgcagcgg cagttcggga ccgtagccct cgatgcgcaa cgtcaccccg
     3181 ggcgcctcga ccgccagccg cgccatcagt ggcgggacca gcagcaccgt gtggatgtcg
     3241 ctggccacca ggcggaccgt ccgggtctcg gcccggggat cgaaggccgg ccggctgaac
     3301 aggccctgca cctcttccag cgccgccgcc agttgcggaa ccaggccctc ggcgaagggg
     3361 gtcagcacga acccgccgcc ctggcggacc agcagggcat cgtccaacag ctcgcgcagc
     3421 cgcccgagcg cccggctcat ggccggctgg ctgagtcccg cgtcggccgc cgcccgggtg
     3481 acgttgcgcc gccgcagcaa ggcctccagc ggcggcagca ggttcaggtc gacggcggac
     3541 aaattcacct cacgcatagc cgagatacaa actatgcatt ttttgtatgc gcaagccggt
     3601 ggcagggtta cgcctcgatc accggggagc ccgccatgtc cgtcaacgcc gcgtcgcccg
     3661 tcgccccgag gatgggctgg ccgttctgga tcgtgctgat cacctcgctg ctgatcgccc
     3721 tggcgtcggt gcgctacctg gttcccggcg cgccgggcgg ggccccggcc atcatggcga
     3781 acgccttcac gcggtacggc gtcctgaccg tccacgccgg gttcgcgatg gtcgccctga
     3841 cgatcgggcc gttccagttc ctgcgcggcc tgaggacccg ccggccgcgg ctgcaccgct
     3901 ggatgggccg gacctatgtg ctggcctgcc tggtcgcggg cgccgcggga ctgctgctgg
     3961 ccttcggggc gaccaccggc ccggtctcga cggccggctt cggcctgctg gcggtgctct
     4021 ggctgtacac cacgaccagg gcctggacct ccgcgcgcgg ccgccggttc gacgagcacc
     4081 gccgctggat gatccgcagc ttcgccctga ccttcgcggc ggtgaccctg cgcctctacc
     4141 tgcccttcgc gttcttctcg ccgttcggct acgacgacac ctaccgcgcg atcagcttcc
     4201 tctgctgggt cccgaacctg ctgttcgccg aatggtacct gcgggcgcgg cctcaggtcc
     4261 cgtagagagc ccaggcgatc agcccgaccg ccagcgccat cagcatccag ccgggatggc
     4321 gaccccgggt ggcccaggca ttgccgagcg cgccgtagag gaaatggctg ccgaccatca
     4381 ggcccaccgc cagccgggcg tccgggctga acgggcagac tgccgccgcg atcaggacca
     4441 gcccgccgag cagccagctg atcgtgctgt agtggagcag gggtgcgacc agccggcgga
     4501 tgaccagcga cacccgccgg tcgccggcga ggatcgtgtc gaaaggccgg gccatcaggc
     4561 gctcgatgag aacgccgtga atcgccgctg tgatgccgcc gaccgcgccg gctgcaatga
     4621 acgccgtgtt ttgccaatcc atgaccatac actcctgtat ggatttcgta tcggcgcgtc
     4681 tctggtccga gtcaatacac ctgtgtatgg ttcacacatg agcgaccgcc tgacgaaagc
     4741 cgactggatc cgccacggcc tgctgaccct ggccagacac ggcccgggcg ggctgaaggt
     4801 cgggcccatg gcgacggcgc tgaaggtctc gcgcggcagc ttctactggc acttcgagga
     4861 catcggcgac ttccgggccc agctgctgca aggctggcgg gaggtgtcca ccgaccagat
     4921 catccaggac ctcgacggga ccgccgcgcc ggaccggctg aagcggctga tgcgccgggc
     4981 gttcggcatg cggctgaccc tggagcgggc cgtgcgggcc tggggggcgg aggaccgtaa
     5041 tgtggcggcg gccgtggcgg ccgtcgacgc ccggcgggtc gggcatatcg cgggcttgct
     5101 ggcgacgtcg ggcgtggacg ccgagcgggc ggggcaacgg gcgaccttcc tct
//
LOCUS       DAORNX010000192         5148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_11984_length_5148_cov_7.554487, whole genome shotgun sequence.
ACCESSION   DAORNX010000192 DAORNX010000000
VERSION     DAORNX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11984_length_5148_cov_7.554487"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLO65_09330"
     CDS             complement(<1..497)
                     /locus_tag="PLO65_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRD28489.1"
                     /translation="MRLAFLGTPDFAVQSLAALLAAGHEVVCVYSQPPAPRGRGQVLQ
                     PSPVHAFAQAHGLPVRTPASMKTAEEVAAFQALDLDAAVVVAFGQILVKAVLDAPRLG
                     CFNVHASLLPRWRGAAPIQRAIMAGDAVTGVQVMRMSEGLDEGAVLATETVRIGPLDT
                     AGTLH"
     gene            600..914
                     /locus_tag="PLO65_09335"
     CDS             600..914
                     /locus_tag="PLO65_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HRD28490.1"
                     /translation="MRTHVIVAIAAVGLALGACSKQDRQEVGEGAAAVGGEVKEAAGE
                     IAHDPDVKEAGAAIGAAGAEAADAVKDTAGEIQEGVADASADARREAEEQRAASGDTS
                     RK"
     gene            complement(1035..1583)
                     /gene="def"
                     /locus_tag="PLO65_09340"
     CDS             complement(1035..1583)
                     /gene="def"
                     /locus_tag="PLO65_09340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRD28491.1"
                     /translation="MSIRRILTVDNAADLAVLKQVSAPVEGGVTDELRALMDDMLETM
                     YAAPGIGLAAVQVGDPRQIIVMDLGDREGVDEGDETGRRNPRYLINPEILWSSEEMAS
                     YEEGCLSVPEVFDDVQRPARVKIRYLNYAGEVIEEDAAGLFAVCVQHEMDHLKGVLFI
                     DHLSRLKRERAIKSVKKAARAA"
     gene            complement(1589..2860)
                     /gene="rmuC"
                     /locus_tag="PLO65_09345"
     CDS             complement(1589..2860)
                     /gene="rmuC"
                     /locus_tag="PLO65_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRD28492.1"
                     /translation="MNDPTVIAIAAAAVFAIAFLGVLVLWLRERGQSVKLATELAGAI
                     ERARLQEDARATMGDYLRAQAEQTATATAEALLKRTDETFRNREALAQQRLDAQLKPV
                     AETLARFEAQVTAVEKARAEETGGLKAQITALMEASTATQAEARKLSAALRRGAGVQG
                     RWGEQTLRNVLDAAGLSAQYDFVEQFSLDTEEGRRRPDVKVRMPAGAVFAIDAKCSLN
                     AFMDAQESTDEAARDAALTRHAQSVRGHMQGLSSKAYWDQFGDERSPDFVAMFVPGDG
                     FLAAALDRMPDLMTEAMDRRVLLVTPTTLFALCKAVAYGWRVEEQAANADKIAALGRE
                     LYKRLSVMGGHAASVGKALEAAVGRYNQFVGSLETQVLTQARRFEDFKVDHEGKDIVQ
                     LEPVETAVRSLNKLAAADAPALTAPDGAPTS"
     gene            complement(2928..3530)
                     /gene="recR"
                     /locus_tag="PLO65_09350"
     CDS             complement(2928..3530)
                     /gene="recR"
                     /locus_tag="PLO65_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRD28493.1"
                     /translation="MASTAGPEIERLIALLGKLPGLGPRSARRAALALLKRREQLLDP
                     LTDAMVEARAKVRTCSTCGSLDTSDPCAICADRARDGGLLCVVEEVGSVWALERASAF
                     RGRYHVLGGLLSALDGMGPDKLRVAELLNRAAGGEVREVILALPATVDGQTTAHYLAE
                     RLAPSGVPVTMLARGVPVGGDLDWLDDGTIAQALRARRPA"
     gene            complement(3714..4523)
                     /locus_tag="PLO65_09355"
     CDS             complement(3714..4523)
                     /locus_tag="PLO65_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28494.1"
                     /translation="MSRANGRKAIFITGAASGMGLETARLFAEKGWYVGGVDVNTDGL
                     RALEQELGWDNCLVRELDVTDRDAYRDTLAAFVEAAGGRLDVIFNNAGIGRGGPFEDM
                     SWEDAMAVININLVAVLSGIHLAIPYLKATPGSLCFSTSSSSAIFGMANIAVYSATKH
                     AVKGFTEALSVELKAAGVRVADTLPGLIDTPILPDEAKAGAPTEGMWRLTPPREVAEA
                     VWSAYHTDKIHWYVPGELEAFDQLQTAQPEMIRDQMVQAGLFNQAAENPPQ"
     gene            complement(4684..>5148)
                     /locus_tag="PLO65_09360"
     CDS             complement(4684..>5148)
                     /locus_tag="PLO65_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRD28495.1"
                     /translation="QARVAAPLPRAAPQTTAALASFDDVLKLIQAKRDITLLMDVQQY
                     VRPISFRPGAIEFEPAPRAPSDLTRRLSSRLKEWTGQTWLVATAGGGGAESAWERQKR
                     EEKEARDQILADPFVMSVMDAFPGAELLGVRQLAVPQGEAVSVNPDEDVEED"
BASE COUNT          760 a         1796 c         1832 g          760 t
ORIGIN      
        1 tcgtgcagcg tcccggccgt gtcgagcggg ccgatgcgca cggtctcggt cgccagcacc
       61 gcgccttcgt cgaggccctc gctcatgcgc atgacctgca cgccggtgac cgcgtcgccg
      121 gccatgatcg cgcgctggat cggcgcggcc ccgcgccagc gcggcagcag cgaggcgtgg
      181 acgttgaaac agcccagccg cggggcgtcg aggaccgcct tcacgagaat ctggccgaag
      241 gcgaccacca cggccgcgtc gaggtccagc gcctggaagg cggcgacctc ctccgccgtc
      301 ttcatcgacg cgggcgttcg cacgggcagg ccatgagcct gcgcgaaggc gtgcaccggc
      361 gagggctgca acacctgtcc gcgcccgcgc ggcgcgggcg gctgggaata gacgcagacc
      421 acctcgtgcc cggccgcgag aagggcggcg aggctctgga cggcgaaatc gggggttcca
      481 aggaaggcca ggcgcatcgg cggggtttag cggggccggc ggccctgcga aaggcgccgg
      541 gaaccggcgg cttcacagat cgttgatcga tggaacgaaa ccgggaaggg aaacctctca
      601 tgcgcacgca tgtcatcgtc gccatcgccg ccgtcggtct ggccctggga gcctgctcca
      661 aacaggaccg ccaggaggtc ggcgagggcg ctgccgcggt gggcggcgag gtcaaggaag
      721 cggccgggga gatcgcccat gatccggacg taaaggaagc cggcgcggcc atcggcgccg
      781 ccggggccga agcggctgac gcggtcaagg acacggccgg cgagatccag gagggtgtcg
      841 ccgacgcctc cgccgacgcc aggcgcgagg ccgaggagca acgggcggcg agcggcgaca
      901 cgagccgcaa gtagacccat tccgctcaac ccggggaagg cccgggccca gatcaaaggg
      961 tcgcgcggcc agccggcaat catcgagcct tgtcgtctcg gtcccggcct tcgccgggat
     1021 gagcggaaac tggattacgc cgcccgggcg gccttcttga cgctcttgat cgcccgttcg
     1081 cgcttcaggc gcgacagatg gtcgatgaac agcacgccct tgaggtggtc catctcatgc
     1141 tggacgcaga ccgcgaacag gccggcggcg tcctcctcga tgacctcgcc ggcgtagttg
     1201 agatagcgga tcttcacccg cgccggccgc tggacgtcgt cgaacacttc cgggaccgac
     1261 aggcagcctt cctcgtagga ggccatctcc tcggaggacc agaggatttc cggattgatc
     1321 aggtagcgtg gattgcgccg gccggtctcg tcaccctcgt cgacgccctc gcggtcgccc
     1381 aggtccatga cgatgatctg ccgcggatcg ccgacctgca cggcggccag gccgatgccg
     1441 ggggccgcgt acatcgtctc cagcatgtcg tccatcagcg cgcgcagctc atcggtcacc
     1501 ccgccctcga ccggggcgga gacctgcttg agcacggcca gatcggcggc gttgtcgacg
     1561 gtgaggatgc ggcggatgga catttcgctc aggaggtagg ggcgccgtcc ggagccgtca
     1621 aggcgggggc gtccgcggcg gccagcttgt tcagcgaccg cacggcggtc tcgaccggct
     1681 ccagctggac gatgtccttg ccctcatggt cgaccttgaa gtcctcgaat cgccgggcct
     1741 gggtcagcac ctgggtctcc agcgagccga cgaactggtt gtagcggccg acggcggcct
     1801 ccagcgcctt gccgacgctc gcggcgtgac cgcccatcac cgacaggcgc ttgtagagct
     1861 cgcggccgag ggcggcgatc ttgtcggcgt tggccgcctg ctcctcgaca cgccagccgt
     1921 aggcgacggc cttgcagagg gcgaacagcg tggtcggggt gaccagcagc acccgcctgt
     1981 ccatggcctc ggtcatcagg tcgggcatgc ggtcgagggc ggcggcgaga aaaccgtcgc
     2041 cgggcacgaa catggccacg aagtcgggcg accgctcgtc gccgaactgg tcccagtacg
     2101 ccttggacga cagcccctgc atgtggccgc gcacgctctg ggcgtggcgg gtcagggccg
     2161 cgtcccgcgc cgcctcgtcg gtggactcct gggcgtccat gaaggcgttc agggagcatt
     2221 tggcgtcgat ggcgaacacg gccccggccg gcatcctgac cttcacgtcg ggccggcggc
     2281 ggccttcctc ggtgtcgagg ctgaactgct cgacgaagtc gtactgggcc gacaggccgg
     2341 ccgcgtcgag cacgttgcgc agggtctgtt cgccccagcg accctgtacc ccggcgcccc
     2401 gccgaagggc ggccgacagc ttgcgcgcct cggcctgggt ggcggtggac gcctccatca
     2461 gggccgtgat ctgggccttc aggcccccgg tctcctcggc gcgcgccttc tcgacggcgg
     2521 tcacctgggc ctcgaacctg gccagggtct cggcgaccgg cttgagctgg gcgtccagcc
     2581 gctgctgggc gagggcttcg cggttgcgga aggtctcgtc ggtgcgcttg agcagggcct
     2641 cggcggtggc cgtggcggtc tgctcggcct gggcccgcag atagtcgccc atcgtcgccc
     2701 gcgcgtcctc ctgcagccgc gcccgctcga tggccccggc cagctcggtg gccagcttca
     2761 ccgactgtcc ccgctcacgc agccacagga ccagcacgcc caggaaggcg atggcgaaaa
     2821 ctgcggcggc ggcaatggcg atgacggtgg gatcgttcat gctccgttcc tagcgggaat
     2881 cgaccccacg gccaatcctc cccgcgaagt cggggaggac aatgggctca cgcgggccgt
     2941 ctggcccgca gcgcctgggc gatggtgccg tcgtcgagcc agtcgaggtc gccgcccacg
     3001 gggacgcccc gcgccagcat ggttaccggc acgcccgacg gcgccagtcg ctcggcgagg
     3061 tagtgggccg tggtctggcc gtcgaccgtg gcgggcaggg cgaggatcac ctcgcgcacc
     3121 tcgccgcccg ccgcgcggtt cagcagttcg gcgacgcgca gcttgtccgg ccccatgccg
     3181 tcgagcgccg acagcagtcc gcccagcacg tgatagcggc cgcgaaacgc gctggcccgc
     3241 tcgagcgccc agaccgagcc gacctcctcg accacgcaca gcaggccgcc gtcgcgggcc
     3301 ctgtcggcgc agatggcgca gggatcggag gtgtcgagcg agccgcaggt cgaacaggtg
     3361 cgcaccttcg cccgagcctc gaccatggcg tcggtcagcg ggtcgagcag ctgttcacgc
     3421 cgtttcagca acgccagcgc cgcccgccgc gccgacctgg gccccagccc cggcagcttg
     3481 cccagcaggg cgatcagtcg ctcgatctcg ggtccggcgg tggaggccat ggattcctct
     3541 aacccaatcc tccccgcgga gcgaagcgaa acggggaggg ggaccgccgg aggcggtgga
     3601 ggggcagcgc cgacgcaagc ggcccaatcc tgaattccgg tctttccggc ggctacagcg
     3661 ccgccccctc caccatgctc cgcatggtcc ccctctccag ggcggtcttc ggcctactgg
     3721 ggaggatttt cggcagcctg gttgaacagc cccgcctgga ccatctggtc gcggatcatt
     3781 tccggctggg cggtctgcag ctggtcgaag gcttcgagct cgcccggcac gtaccaatgg
     3841 atcttgtcgg tatggtaggc ggaccagacc gcctcggcga cctcgcgcgg cggggtcagg
     3901 cgccacatgc cctcggtcgg ggcgcccgcc ttggcctcat cgggcagaat tggcgtgtcg
     3961 atcaggcccg gcagggtgtc ggcgacccgc acgccggcgg ccttcagctc gaccgagagc
     4021 gcctcggtga agcccttcac cgcatgtttg gtcgccgaat agacggcgat gttggccatg
     4081 ccgaagatcg ccgagctcga cgaggtcgag aagcacagcg aacccggggt ggccttcagg
     4141 tagggaatgg ccaggtggat gccgctgagc acggccacca ggttgatgtt gatcaccgcc
     4201 atggcgtcct cccaggacat gtcctcgaac ggaccgcccc ggccgatgcc ggcgttgttg
     4261 aatatcacgt cgagccggcc gcccgccgcc tcgacgaagg ccgcgagcgt atcgcggtag
     4321 gcgtcgcggt cggtgacgtc gagctcgcgg accaggcagt tgtcccagcc cagttcctgc
     4381 tccagcgcgc gcaggccgtc ggtgttgacg tcgacgccgc cgacgtacca gcccttctcc
     4441 gcgaacagcc gcgccgtctc caggcccatg cccgaggcag cgcccgtgat gaagatcgcc
     4501 ttgcggccgt tggccctgga catggttcaa ctcccttgat ggccagcgca tctgatggcg
     4561 cgggtccatg gtgcgtggtt cgacacgcgc ttcgcgctgc tcaccatgac gtggggggcg
     4621 acatctcaga tccacgtcat cctgagcagg gccgaaggcc cgtgtcgaag gacgcaggga
     4681 gggtcagtcc tcctcgacgt cctcgtcggg attgacgcta accgcttccc cctgcggaac
     4741 cgcaagctgt cgcacgccaa gcagctcagc gccggggaag gcgtccatca ccgacatcac
     4801 gaacgggtcg gcgagaatct ggtcacgggc ttccttctcc tcgcgcttct gccgttccca
     4861 ggcgctttcg gccccgccgc cgccggcggt ggccaccagc caggtctggc cggtccattc
     4921 cttgaggcgc gacgacagcc gccgggtcag atccgacggc gcgcgcggcg cgggttcgaa
     4981 ctcgatggcc ccggggcgga agctgatcgg ccggacgtac tgctgcacgt ccatcagcag
     5041 ggtgatgtcg cgcttggcct ggatcagctt caacacgtcg tcgaaggagg cgagggccgc
     5101 ggtggtctgg ggcgcggccc ggggcagggg cgcggcgacc cgggcctg
//
LOCUS       DAORNX010000193         5140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12002_length_5140_cov_7.820846, whole genome shotgun sequence.
ACCESSION   DAORNX010000193 DAORNX010000000
VERSION     DAORNX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12002_length_5140_cov_7.820846"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="PLO65_09365"
     CDS             complement(<1..1503)
                     /locus_tag="PLO65_09365"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRD28496.1"
                     /translation="MLQRMGLGLALAASVMLAGCAVDKAPAARHVLPTDVTPVHYDIT
                     VRPDATALTFTGSVGADVQVARATDTVTLNAADLTFGKVRLDGAEASPRVAYDADAQT
                     ATLTFAQPLSAGPHRLAIDYAGKINRQAFGLFSVDYDTAQGPRRLLATQFESPDARRF
                     APMWDEPSLKATFRLTVEAPAADMVVSNMPVQAAAPIEGGLTRTTFAVSPKMSSYLLF
                     LAVGDFERVSRKVGDVDVGVVVKRGSTGEAGFALDAAEKLLPFYDDYFGTPYPLPKLD
                     LVAVPGGAAAFSAMENWGAILYFEKAVLVDARVSSEADRQNAFTTIAHEMAHQWFGDL
                     VTMSWWDDIWLNEGFASWMETKATAAFHPEWSVWLQDMAARDGAMNLDARSATHPVVQ
                     DVADARDTAFDTISYQKGQAVIRMVEAWVGEDHFREALRAYMKKHAYGNTVSDDLWSA
                     VEAASGQPIRRIAADFTGQPGVPLIRVTSLGSAWRLAQRRFGVDAPSRQPL"
     gene            1562..2167
                     /locus_tag="PLO65_09370"
     CDS             1562..2167
                     /locus_tag="PLO65_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRD28497.1"
                     /translation="MKDLSGFTLLPGRLDPAAQEALVETVLAAARGAPFHRQVTPGGK
                     AMSVEMTGLGPLSWVTDARGYRYEARHPLTGRPWPPMPPVLQELWAELVDPAVPADAC
                     LVNLYRESARMALHRDEDEADFRFPVLSVSLGDTAIFRLGGPRRTDPTRSVRLASGDV
                     CLLGGDSRLAFHGVDRIIPGSSRLVPGGGRINLTLRRARSG"
     gene            2237..3124
                     /locus_tag="PLO65_09375"
     CDS             2237..3124
                     /locus_tag="PLO65_09375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD28498.1"
                     /translation="MLNLTRPEGSEPRDFDLSRRALPGLFFAGFAAAAVSASAEPIHT
                     DAAGLIAETVLVPAGDRDIPAYVARPDGRGRRPVVLVVSEVFGIHEYIRDVCRRLAKL
                     GYVAIAPDFFVRYGDPAPLTDFAAIRGIVAAATDAQVMGDIDATTRWLSAQAFVNRRR
                     MAITGYCWGGAQVWLACERFRAFRCGVAWYGRLSRPAAGQFLAEPERQWPIELAGYLK
                     APVLGLYGGRDQGIPAADIEAMRARLAEFRKRGSEIIVYPDAQHGFHADYRASYDATA
                     AQDGWRRMIDFFHENGVRP"
     gene            complement(3225..4658)
                     /gene="dnaA"
                     /locus_tag="PLO65_09380"
     CDS             complement(3225..4658)
                     /gene="dnaA"
                     /locus_tag="PLO65_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRD28499.1"
                     /translation="MTSMGGRMMSSQTPAGAVWTKACLALRRELGEAAFGSWLAQAAL
                     REIADGRIILVTPTGVARDWIRRNAWRRIGELWAEHDPAGRSLDLKSRMEFEADTGGL
                     DIIAEAIEVMDPVATDAPTIAPTPGLRTGRITGLQDRFTFDSFVPGPANEFAHAVARR
                     VASWADGHFNPVVIHSGYGFGKTHLLNAMAWEAMRAAPEKRVVYLSAERFLSTFVRAV
                     MDRSTALFKEELRAADLLLIDDVHFVAGKQSTQEELFHTLTALMEDGRRVVFSADRPP
                     AQITEMDARLRSHLSAGLVCGIEPADRTLRMGILERKLIALGRQGGFTASARPEVMQF
                     LADRFTDSVRELEGALNTLVARMGAQVGGLSLDEAQAILRPHLRGAEKRITIDDIQKA
                     TSEHFGLRQADLVSERRNRSVARPRQAAMWLAKQLTTRSLPDIGRRFGGRDHTTVLHA
                     VRRIEALKAEDPQLSRDLEALTRKLRG"
BASE COUNT          760 a         1767 c         1835 g          778 t
ORIGIN      
        1 gagcggctgg cgcgacgggg cgtcgacgcc gaagcgccgc tgggccagcc gccaggccga
       61 gccgaggctg gtcaccctga tcagcggcac cccgggctgg ccggtgaagt cggcggcgat
      121 gcggcggatg ggctggcccg aggcggcttc caccgcgctc cacaggtcgt cgctgaccgt
      181 gttgccgtag gcgtgcttct tcatgtaggc gcgcagggcc tcgcggaagt ggtcctcgcc
      241 gacccaggcc tcgaccatgc ggatgaccgc ctggcccttc tggtagctga tggtgtcgaa
      301 ggccgtgtct cgggcgtcgg cgacatcctg gacgacggga tgggtggccg accgggcgtc
      361 gaggttcatc gccccgtcgc gcgcggccat gtcctgcagc cagaccgacc actcgggatg
      421 gaaggccgcc gtggccttgg tctccatcca ggaggcgaag ccctcgttga gccagatgtc
      481 gtcccaccag ctcatcgtca ccaggtcgcc gaaccactgg tgggccattt cgtgggcgat
      541 ggtggtgaag gcgttctggc ggtcggcctc gctggagacc cgggcgtcga ccagcacggc
      601 cttctcgaaa tagaggatcg ccccccagtt ttccatggcc gagaaggccg ccgcgccgcc
      661 gggcacggcc accaggtcca gcttgggcag gggatagggc gtgccgaaat agtcgtcgta
      721 gaagggcagc agcttctcgg ccgcgtcgag ggcgaagccc gcctcgccgg tcgagccgcg
      781 cttgaccacc accccgacgt cgacgtcgcc gaccttgcgg ctgacccgct cgaaatcgcc
      841 caccgccagg aacagcagat aggaactcat cttcggcgag accgcgaagg tggttcgggt
      901 caggccgccc tcgatcggcg cggccgcctg gacgggcatg ttggacacga ccatgtcggc
      961 ggccggggcc tcgacggtca gccggaaggt cgccttcagc gacggctcgt cccacatcgg
     1021 cgcgaaccgc cgcgcgtcgg gggattcgaa ctgggtggcc agcagccgcc gcggcccctg
     1081 ggccgtgtcg tagtcgaccg agaacaggcc gaacgcctgc cggttgatct tgccggcgta
     1141 gtcgatcgcc agccggtgcg ggccggcgga caggggctgg gcgaaggtca gggtggcggt
     1201 ctgggcgtcg gcgtcatagg cgacccgggg cgaggcctcg gcgccgtcga gccgcacctt
     1261 gccgaaggtc aggtcggcgg cgttgagggt caccgtgtcg gtggcccgcg ccacctggac
     1321 atcggcgccg accgagccgg tgaaggtcag ggcggtcgcg tcgggccgca cggtgatgtc
     1381 gtagtggacg ggcgtgacgt ccgtgggcag cacatggcgc gcggccgggg ccttgtccac
     1441 ggcgcagccc gccagcatga ccgacgccgc cagcgccagg cccagtccca tgcgttgcaa
     1501 caccgaaagt ctccccttgc cgcacggccg gaggttagac tgatcccgtg cggactgaat
     1561 catgaaggat ttgtcaggtt tcaccctgct gccggggcgg ctggacccgg ccgcccagga
     1621 ggcgctcgtc gagaccgtgc tggcagccgc gcgcggggcg cccttccatc gccaagtgac
     1681 gcccggcggc aaggcgatga gcgtggagat gacgggcctg gggccgctga gctgggtcac
     1741 ggacgcgcgc ggctatcgct acgaggcccg ccacccgctg acgggccggc cctggccgcc
     1801 gatgccgccc gtgctgcagg aactctgggc cgagctcgtc gacccggccg tccccgctga
     1861 cgcctgcctg gtcaatctgt atcgcgagag cgcgcggatg gccctgcacc gcgacgagga
     1921 cgaggccgac ttccgcttcc cggttctgtc ggtctccctg ggcgacacgg cgatcttccg
     1981 ccttggcggc ccccggcgca ccgatccgac gcgttcggtg cggctggcct ccggcgacgt
     2041 ctgcctgctg ggcggggact cgcggctggc gttccacggc gtggaccgga tcatccccgg
     2101 ctcgtcgcga ctggtgccag gcggcggccg gatcaatctg acgctgcgaa gagcgcggag
     2161 cggctgagcg gctttccagc gcgacgactc cggcgttagg ctagcttatg gcctgcccaa
     2221 aaccggaaac cccgccatgc tgaacctcac ccgtcccgaa gggtcggagc cgcgtgactt
     2281 cgacctgtcg cgccgcgccc tgcccgggct gttcttcgcc ggcttcgcgg ccgccgcggt
     2341 gtcagcgtcc gccgagccga tccacaccga cgccgccggg ctgatcgccg agacggtgct
     2401 ggtcccggcc ggggatcgcg acattcccgc ctacgtcgcc cgtcccgacg ggcgcgggcg
     2461 gcggccggtc gtgctggtgg tgtcggaggt gttcggcatc cacgaataca tccgcgacgt
     2521 ctgccggcgg ctggcgaagc ttggctatgt agccatcgcg ccggacttct tcgtacgcta
     2581 cggcgacccc gcgccgctga ccgacttcgc cgccatccgc ggcatcgtcg cggcggccac
     2641 cgacgcccag gtgatgggcg acatcgacgc gaccacgcgg tggctttcgg cccaggcctt
     2701 cgtcaaccgg cgcaggatgg ccatcaccgg ctactgctgg ggcggcgccc aggtctggct
     2761 ggcctgcgag cggttccggg cctttcgctg cggcgtggcc tggtacggcc ggctgtcgcg
     2821 gccggcggcc ggccagttcc tcgccgagcc cgagcggcag tggccaatcg agctggccgg
     2881 ctacctcaag gcgccggtgc tgggcctcta tggcggcagg gaccagggca tccccgcggc
     2941 cgacatcgag gccatgcggg ccaggctggc cgagttccgc aaacgcggca gcgagatcat
     3001 cgtctatccc gacgcccagc acggcttcca cgccgactat cgggccagct acgacgcgac
     3061 cgccgcccag gacggctggc gacggatgat cgacttcttc catgagaatg gcgtgaggcc
     3121 gtaggggccg gggacatgta ccgcaggtac gtgtccccta atccggattc acaggcagct
     3181 cgattggggg acacgtaccg gcttcgccgg ctacatgtcc ccgcctaccc ccgcagcttg
     3241 cgcgtcaggg cttcgaggtc gcgcgacagc tgcggatcct cggccttgag cgcctcgatg
     3301 cggcgcacgg cgtgcaggac ggtggtgtgg tcgcgaccgc cgaagcgccg gccgatgtcc
     3361 ggcagcgagc gggtggtcag ctgcttggcc agccacatgg ccgcctgacg ggggcgggcg
     3421 accgagcggt tgcggcgctc cgagacgaga tcggcctggc gcaggccgaa gtgctccgac
     3481 gtggccttct ggatgtcgtc gatggtgatg cgcttctccg cgccgcgcag gtgcggccgc
     3541 aggatggcct gggcctcgtc cagcgacagg ccgccgacct gggcgcccat gcgggccacc
     3601 agggtgttca gggcgccttc cagctcacgc acgctgtcgg tgaagcggtc agcgaggaac
     3661 tgcatcacct cgggcctggc cgaggcggtg aacccgccct ggcggcccag ggcgatcagc
     3721 ttgcgttcga ggatgcccat gcgcagcgtg cggtcggccg gctcgatgcc gcagaccagg
     3781 cccgccgaca ggtggctgcg caggcgcgcg tccatctcgg tgatctgggc cggcggccgg
     3841 tcggccgaga acaccacccg gcggccgtcc tccatcaggg cggtcagggt gtggaacagc
     3901 tcttcctggg tcgactgctt gccggccacg aaatgcacgt cgtcgatcag cagcaggtcg
     3961 gcggcgcgca gctcttcctt gaacagggcc gtcgagcgat ccatcaccgc ccgcacgaag
     4021 gtcgacagga agcgctcggc gctcagatag acgacgcgct tttccggggc cgcccgcatg
     4081 gcctcccagg ccatggcgtt gagcaggtgg gtcttgccga agccgtagcc gctgtggatc
     4141 accaccgggt tgaagtggcc gtcggcccag ctggccaccc ggcgggcgac ggcgtgggcg
     4201 aactcgttgg ccggaccggg cacgaagctg tcgaaggtga agcgatcctg caggcccgtg
     4261 atccgtcccg tccgcagccc cggcgtcggc gcgatggtcg gggcgtcggt ggcgaccggg
     4321 tccatcactt cgatggcctc ggcgatgatg tccaggccgc cggtgtcggc ctcgaattcc
     4381 atgcgcgact tcaggtcgag gctgcggccg gccgggtcgt gctcggccca cagctcgccg
     4441 atgcggcgcc aggcgttgcg gcggatccag tcgcgggcga cgccggtggg ggtgacgagg
     4501 atgatccggc cgtcggcgat ctcgcgcagg gcggcctggg ccagccagga gccgaacgcc
     4561 gcctctccga gctcccgccg aagcgccaga caagccttcg tccagaccgc gcctgcagga
     4621 gtctgcgagg acatcattcg cccgcccatg gaagtcatca gccccacctg ttggttactc
     4681 tttccgaacc cgcgaaaccc ctccgccccc acgacggaga agtcataaac tcaacgcaga
     4741 caaggactta accggcggat gcacgacatc cgccgccgcc tttcgcatac ccaaaattgg
     4801 aaatggacca agcgtcgagg cttcacactt agcggcccaa aaaaccgtgg gtcaaacgtg
     4861 aaatttgagt ccggacgcgg atatttctgt tgactcacga gggcccctcg cgtggcaagg
     4921 gaatccgtta acgacccctc cgggaggtgt gagaattcgc cgcacacggg aaacgcgaac
     4981 atgaaaaacc ggccggcgcg agaggcgcct gccggttgat caagctgctg aaatataagg
     5041 cgaacggcgt tagccgaccg gtgtctcgcg agccgaaatc aggccgccga cagcttcttc
     5101 agctgggcgt tcaggcgcga gaccttgcgc gagccggtgt
//
LOCUS       DAORNX010000194         5085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12202_length_5085_cov_8.436581, whole genome shotgun sequence.
ACCESSION   DAORNX010000194 DAORNX010000000
VERSION     DAORNX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12202_length_5085_cov_8.436581"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /gene="gluQRS"
                     /locus_tag="PLO65_09385"
     CDS             complement(<1..649)
                     /gene="gluQRS"
                     /locus_tag="PLO65_09385"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRD28500.1"
                     /translation="MSASFVTRFAPSPTGRLHRGHAFSALTAFTAAQRARGRFLLRIE
                     DIDVTRCRPEHEAAILDDLAWLGLDWEQPVRRQSEHLADYEAALESLRARCLVYRCFR
                     TRKDLAQAALSAPHGAESPFRGGPIHPNEEDRWLSEGKPFAWRLSLDAAREALGGFHR
                     LRFQERGEGPNGEHGRIEARPERAGDVVLARKDVGVAYHLACVVDDALQEITEVIR"
     gene            complement(698..1120)
                     /locus_tag="PLO65_09390"
     CDS             complement(698..1120)
                     /locus_tag="PLO65_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRD28501.1"
                     /translation="MLISEVMTANPQVARPSDTVADVARRMNATDAGAIPVVEDGQIR
                     GIITDRDIVLRVVGEQRSFDTRVEEVMSTDVQTCREDAPIADVIFQMTDKQIRRILIV
                     DGADRLTGIVSLGDVAQEASARKAGAVLEDISEAPANN"
     gene            complement(1194..1823)
                     /locus_tag="PLO65_09395"
     CDS             complement(1194..1823)
                     /locus_tag="PLO65_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28502.1"
                     /translation="MREPATAEARPYHHGDLRRALVDAARRILENEGPSALSLRAVAR
                     EAGVSPAAPYHHFKDKGELLDAVAHVGWDMLGESMTGARARAANLGALMTEIGVSYVQ
                     FSQQHPALYRVMYDCSRDKDMLPEGMQDNEDSAYCMVRDTFRKVAGPEVKDDDIELAT
                     IAAWCAAHGLAEMSSFKQFDPLKSALGGEEAFLRGVLGHLGMFQASRPA"
     gene            complement(2220..3401)
                     /locus_tag="PLO65_09400"
     CDS             complement(2220..3401)
                     /locus_tag="PLO65_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HRD28503.1"
                     /translation="MRSDLGMGIVLVVAGLLLVGCLRPPQAPAPPGITLTPLRSISAL
                     EARVLLRLAGVKGLPVRYAVDCYRMEYVVTGPDGRPARLSGLLALPRGVAASRLVSFQ
                     HGTATTRSAVPSQPDGTGLAAAILFAGNGYALVAPDYPGMGVSPGKHPYYVAESIGPS
                     VVAMIDAARRLDGVPDAPVFLSGFSEGGWASVVALKILEARGERVLGAAPVAGPYDVR
                     RISIPAAMKGGSPSHSLYLAYAAWGQAGYYGNRLDSLLTPEYAATVERLFAGAKPKAI
                     LAGLPADPRLMFSPAFLALYDRGEPHWYLDTFAAAGVADLTPVTPVRLYYGSQDRDVI
                     PEEARAAERGMRSRGADVTAVDVGPVGHDASMLAAAPLIFAWLRQLEVAETMTGTGSP
                     G"
     gene            3628..4203
                     /locus_tag="PLO65_09405"
     CDS             3628..4203
                     /locus_tag="PLO65_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRD28504.1"
                     /translation="MTPHRAQVLKAPPSGWPALVLNADYRPLSYYPLSLWPWQEVIKA
                     VFLDRVEVISTYDKMVHSPSFEMRLPSVVCLKSYVAQDRPPAFTRFNLFLRDSFSCQY
                     CGAGSDLTFDHVIPRSRGGRTTWENIVTACARCNLTKGGRTPKEAHMHPHRFPRRPSM
                     HELQDHGRRFPPGFLHESWLDYLYWDIELEA"
     gene            4273..4617
                     /locus_tag="PLO65_09410"
     CDS             4273..4617
                     /locus_tag="PLO65_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRD28505.1"
                     /translation="MSASQSRSVERRHGGRARALLSGKLCFEDATKTIDCVIRNITPG
                     GAMVETPTPQLVPGRLQLMQVSEGIVWDADVAWRRGARIGLVLHDRHDLRESTDKQLR
                     VLRAIWGQMALR"
     gene            complement(4626..4766)
                     /locus_tag="PLO65_09415"
     CDS             complement(4626..4766)
                     /locus_tag="PLO65_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28506.1"
                     /translation="MPDPAPPTPPDPAPPYEPPPPEVDPGLPGEPSPAPAYDPPEPPT
                     VA"
     gene            complement(4800..>5085)
                     /locus_tag="PLO65_09420"
     CDS             complement(4800..>5085)
                     /locus_tag="PLO65_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HRD28507.1"
                     /translation="FALGAGTALLGDKAAHACTEAVETVIEGHYAGQIAELADRDPAL
                     AAELTKFRDEELAHRDIARDEGAREAPGYPLLAAVIRAGCKAAIRISEKV"
BASE COUNT          754 a         1771 c         1767 g          793 t
ORIGIN      
        1 cgcggatcac ctcggtgatc tcctgcaggg cgtcgtcgac gacacaggcc aggtgatagg
       61 cgacgccgac atccttgcgc gccagcacca cgtcgccggc ccgctcgggt cgggcctcga
      121 tgcggccgtg ctcgccgttc ggcccctcgc cccgttcctg gaaccgcagc cggtggaagc
      181 cgccgagcgc ctcgcgcgcc gcgtcgagcg acagccgcca ggcgaacggc ttgccctccg
      241 acagccagcg gtcctcctca ttgggatgga tcggcccgcc gcggaagggc gactcggcgc
      301 cgtgtggcgc gctcagcgcc gcctgggcca ggtccttgcg ggtcctgaaa cagcgataga
      361 cgaggcaccg cgcccgcagg ctttcgagcg ccgcctcata gtcggccagg tgctcggatt
      421 gccggcgcac gggctgttcc cagtcgagcc ccagccaggc cagatcgtcg aggatcgccg
      481 cctcgtgctc gggccggcag cgagtcacgt cgatgtcctc gatgcgcagc agaaagcggc
      541 ccctggccct ctgcgccgcc gtgaaggcgg tcagggccga gaaggcatgg ccgcgatgca
      601 gccggcccgt gggggacggg gcgaaacggg tgacgaagga ggcgctcacc ggccgtctgt
      661 agaccggcgc gcgccgcttc gcatcagtcg tgcgtcatca gttgttggcg ggagcctcgg
      721 aaatgtcctc gagcaccgcg ccggccttgc gggccgaggc ctcctgggcg acgtcgccga
      781 gcgaaacgat gccggtcagc cggtcggcgc cgtcgacgat caggatgcgg cggatctgct
      841 tgtcggtcat ctggaagatg acatcggcga tcggggcgtc ctcgcggcag gtctggacat
      901 cggtgctcat cacctcctcg acccgggtgt cgaagctgcg ctgctcgcca accacgcgca
      961 gcacgatgtc gcggtcggtg atgatgccgc gaatctggcc gtcctcgacg accgggatgg
     1021 cgccggcgtc ggtggcgttc atgcggcggg ccacatcggc gacggtgtcg gagggccggg
     1081 cgacctgcgg gttggcggtc atcacttcgg agatcagcat ggggatactc tcggcgactg
     1141 ggggtccagc acgcctaacg gccggaaccc gccgccgttc cccgcgtccg ggctcaggcg
     1201 ggccttgacg cctggaacat gcccagatgg ccgagcacgc cgcgcaggaa ggcttcctcg
     1261 ccgccaagcg cgctcttcag cggatcgaac tgcttgaagc tgctcatttc cgccaggccg
     1321 tgggcggcgc accaggcggc gatggtggcc agctcgatgt cgtcgtcctt gacctcgggc
     1381 ccggccacct tgcggaaggt gtcgcggacc atgcagtagg cgctgtcctc gttgtcctgc
     1441 atgccttcag gcaacatgtc cttgtcgcgc gagcagtcgt acatcacgcg atacagggcc
     1501 ggatgctgct gtgagaactg cacgtaggac acgccgatct cggtcatcag ggcgccgagg
     1561 ttggcggcgc gcgccctggc cccggtcatg gactcgccca gcatgtccca gcccacatgg
     1621 gccacggcgt cgagcagctc gcccttgtcc ttgaagtggt gatagggcgc ggccggactg
     1681 accccggcct cccgcgccac cgcccgcagc gacagggcgc tgggcccttc gttctcgaga
     1741 atccggcgag cggcgtccac gagggcgcgc ctcagatcgc cgtgatgata gggccgggcc
     1801 tcggcggttg caggttctct catggggctc acgttaaatc tctatccgaa cgctgtaaag
     1861 atcatcttga cgccgttcag atcggtgtta tttaagcacc gttcagatcg aacggccccc
     1921 gctcccccgg gggttcaaac tggggggctc cgtccagaac aggggcccaa acttaaggac
     1981 tgtgtgtcat gaccatctcc aagctcatca gcttcgaacg cttcttcgac cgctcggcgg
     2041 tcgtcttctt cctggtgctg ggcgccgccg ccgccggcgc gaccgccttc gccggggcgt
     2101 aacccccgcg acgacccttc gatcccgttt cgtccccccg atcccccggg gtggacggcg
     2161 ggaccgaacc tgacaagggc cgcatcgcgc ggcccttttt ctttgccttg aggtcaggct
     2221 cagccgggcg atcctgtccc ggtcatcgtc tcggccactt ccagctgacg cagccaggcg
     2281 aagatcaggg gggcggcggc cagcatcgag gcgtcgtgcc ccaccgggcc cacatcgacg
     2341 gccgtcacat cggccccccg gctgcgcatg ccccgctcgg cggctcgggc ttcctcgggg
     2401 ataacgtccc ggtcctgcga tccgtaatag agtcgcaccg gcgtgaccgg cgtcagatcg
     2461 gcgacccccg cggcggcgaa ggtgtccaga taccagtggg gctcgccgcg atcgtagagc
     2521 gccaggaagg cggggctgaa catcagcctg ggatcggccg gcaggccggc gaggatcgcc
     2581 ttgggcttgg ctccggcaaa cagccgctcc accgtggcgg cgtattccgg cgtcagcagg
     2641 ctgtcgagcc ggttgccgta gtagcccgcc tggccccagg cggcgtaggc cagatagagc
     2701 gagtgcgagg gcgacccgcc cttcatcgcc gccgggatcg agatgcgtcg aacgtcgtac
     2761 ggaccggcga caggggccgc gcccaggacc cgttcgccgc gcgcctcgag gatcttcagg
     2821 gcgacgacgc tggcccagcc gccctcggag aagcctgaca ggaacaccgg cgcgtccggg
     2881 acgccgtcga gacgtcgggc ggcgtcgatc atcgcgacca ccgagggccc gatcgattca
     2941 gcgacgtagt agggatgttt gccgggcgag acgcccatgc ccggatagtc gggagccacc
     3001 agcgcatagc cgttgcccgc gaacaggatc gccgccgcca ggcccgtgcc gtcgggttgt
     3061 gacgggacgg cggagcgggt cgtggcggtg ccgtgctgaa agctcacaag acggctggcc
     3121 gcgacgccgc ggggcagggc cagcaggccg ctcaggcgcg ccggccggcc gtcggggccg
     3181 gtgacgacgt attccatccg gtagcagtcg acggcatagc ggacgggcag acccttcacc
     3241 ccggccaggc gaagcaggac cctggcctcc agggcggaaa tcgatctgag cggggtcagc
     3301 gttatccctg ggggcgccgg cgcctgtggc ggtcgcagac atccgaccag cagcagccct
     3361 gcgacgacca gaacgatccc catccccagg tccgagcgca tcgccggctc cccgcgttgt
     3421 ggcggcccac accctaggcg accgggcagc ccaaatccac aatggtcgcg gcggcggcgt
     3481 tctgtgtctt ggccccgtca cgaaacaggg gctatcctgc gagtcgcgtt cgggggtgtt
     3541 ggcgatcccc gaggaggcgc ggaaagcgag gcaggtcttc aggtccgtcg cgtattcaag
     3601 tcccgagccg tcgtgggatg gtcgcccatg acgccgcata gagcccaggt gctcaaggcg
     3661 ccgccgtccg gctggcccgc cctggttctg aacgccgact accggccgct cagctactat
     3721 cccctgtcgc tctggccctg gcaggaagtc atcaaggcgg tgttcctcga tcgggtcgag
     3781 gtcatctcca cctatgacaa gatggtccac agcccgtctt tcgagatgcg gctgcccagc
     3841 gtggtctgcc tcaagtccta cgtcgcccag gaccgacctc cggccttcac ccgcttcaac
     3901 ctgttcctgc gcgacagctt cagctgccag tactgcggcg cgggctcgga cctgaccttc
     3961 gatcatgtca ttccgcgctc gcggggcggg cgcacgacct gggagaacat cgtcaccgcc
     4021 tgcgcgcgct gcaacctgac caagggcggc cgcacgccga aggaggcgca catgcatccg
     4081 caccgcttcc cgcgccgccc ctcgatgcat gaactgcagg accatggccg ccgtttcccg
     4141 cccgggttcc tgcacgaaag ctggctcgac tacctgtact gggacatcga actggaggcg
     4201 tgatcgtcca ggcggtcagg cgaccgtggt ccggaaagac cttggaaacc tcggcgcgat
     4261 aggctcgcgc ccatgtccgc ttcccagtcc agatccgttg aacgccgcca cggcgggcgc
     4321 gcccgcgcgc tcctgtcggg caagctctgc ttcgaagacg ccaccaagac catcgactgc
     4381 gtcatccgca acatcacgcc gggcggcgcg atggtcgaga cgccgacgcc gcagctggtt
     4441 ccgggccgtc tgcagctgat gcaggtcagc gagggcatcg tctgggacgc ggacgtggcc
     4501 tggcggcgcg gcgcgcggat cggcctggtg ctgcacgatc gtcatgacct gcgcgagtcg
     4561 accgacaagc agctgcgtgt gctgcgcgcc atctgggggc agatggccct gcgatagccc
     4621 atccgtcagg cgacggtcgg cggctctggc ggatcatagg ccggcgccgg gctcggctcc
     4681 ccgggcagac ccggatcgac ttcgggcggg ggcggctcgt agggcggcgc gggatcgggc
     4741 ggagtcggcg gagcgggatc gggcatgccc cctcaatccc cgaacgcgcg ctgcggttcc
     4801 tagaccttct cgctgatgcg gatcgcggcc ttgcagccgg cgcggatcac ggcggccagc
     4861 agcggatagc cgggcgcctc gcgcgcgccc tcgtcgcggg cgatgtcgcg gtgggccagc
     4921 tcctcgtcgc ggaatttcgt cagctcggcg gccagcgcgg gatcgcggtc ggccagttcg
     4981 gcgatctggc cggcgtaatg accctcgatc accgtctcga ccgcctcggt gcaggcgtgg
     5041 gcggccttgt cgccgagcag ggcggttccg gcgccgagcg cgaag
//
LOCUS       DAORNX010000195         5080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12225_length_5080_cov_7.125771, whole genome shotgun sequence.
ACCESSION   DAORNX010000195 DAORNX010000000
VERSION     DAORNX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12225_length_5080_cov_7.125771"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLO65_09425"
     CDS             complement(<1..989)
                     /locus_tag="PLO65_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28508.1"
                     /translation="MLSVKSIHPSLLSPQELAAWRALVAAQPAFGSPLLGPDFALAVG
                     AVREDARVAVYRAGETIVGFLPHHRRPGGLARPLGAPLSDYHGLIAGPDFGLTGARAL
                     ELAGLSAYRFTGLVDPHGMFQDAAAGAASGYAITLTISPEAYLESLRAVSPKRFKNWR
                     RLDNKMERELGPVRIEASDEPAVYEQIMDWKREQLIRTGVADFLRPDWTHRLLAGFLA
                     ATGPLRGLTLVLWAGDRIAAGHFGVTQNGWYHPWVASTNPDLGEFSPGQTFLMRAIAA
                     MPALGLTTYDLGPSHDHYKRHYAAPSQAVGEGTAVAATAAGRLAGGRERIWTLA"
     gene            complement(1068..1565)
                     /locus_tag="PLO65_09430"
     CDS             complement(1068..1565)
                     /locus_tag="PLO65_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRD28509.1"
                     /translation="MAEAFNDLTLSADREARYAEVAAEIASVLKGEPNLTARMATVAS
                     MLAASFDDYFWTGFYVVDPDRTDELVVGPYQGTLGCLRIAFGRGVCGAAAASGETQLV
                     PDVEAFPGHIACDSRSRSEVVVPVFGPDGRLIAVFDVDSDRPAAFDETDARWLERILA
                     DVFSR"
     gene            1604..2014
                     /locus_tag="PLO65_09435"
     CDS             1604..2014
                     /locus_tag="PLO65_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRD28510.1"
                     /translation="MAYWLVKSEPDVFSWDDLVAAGPKGEPWNGVRNHTAKLNMMAMR
                     LGDGVFFYHSQAGKEIVGVAEVVREAYPDPTDAKGVFQCVDLRAVEPLARPFTLAQAK
                     ADPALAAMSLVTSFRLSVQPVTAAEWAHILAAAR"
     gene            complement(2097..3335)
                     /locus_tag="PLO65_09440"
     CDS             complement(2097..3335)
                     /locus_tag="PLO65_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD28511.1"
                     /translation="MSEAWIIDAVRTPRGIGKYGKGALSEMHPQQLGATVLKALAERN
                     KINTAEVDDVIWGTSSQRGPAAGDLGRMSALDAGYDIKSSGVTLDRFCGSGITTVNFA
                     ASTIMAGMSDMVVAGGCEMMSMPNRRSPEDGPVTMDQGNLRLRARHPQSHQGICADSI
                     ATLEGISREALDALALVSQQRAAVAIAENRFAKSLIPVYKEDGTLALDHEEFPRPQTT
                     AEGLAALKPSFEALADVPLDKDGTTFRKLITAVYPDLDLKFFHHAGNSSGVVDGAAAL
                     LLASPAYAKANGMTPRARVVAMANMGDSPTLMLNAPVPAAKKVLAKAGLTVEDIDLWE
                     INEAFSVVAEKFIRDLKLDRSKVNVNGGAMALGHPIGATGSILIGTIVDELERTGKRY
                     GLVTMCAAGGMAPAIIVERM"
     gene            complement(3377..4159)
                     /locus_tag="PLO65_09445"
     CDS             complement(3377..4159)
                     /locus_tag="PLO65_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28512.1"
                     /translation="MKLDSTISAVITGGASGLGAATARRLAADGVRVAIFDLNEELGE
                     ALARELGGVFCKVNVASGEEVDAAFAKARAAIGQERILVNCAGVGGGAKTVSRDKQTG
                     EVKEYPLDNFERILQINLVGTFRCITKSAAGMLTLDPLPDGDRGAIVNTASVAAEDGQ
                     IGQVAYTASKAGIVGMTLVIARDLSNEAIRVNTILPGIFDTPLLGRAPEHVKAGLAAQ
                     VPFPRRLGDPNEYAHLAHTMITNGYFNGEDVRLDGAIRMTPR"
     gene            complement(4290..4487)
                     /locus_tag="PLO65_09450"
     CDS             complement(4290..4487)
                     /locus_tag="PLO65_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRD28513.1"
                     /translation="MKLNEGAVDRVLRIIVGLVVLSLAFVGPKTAWGYLGAIPLLTGI
                     VGFCPLYAVLGINTCKAPRAK"
     gene            complement(4554..4757)
                     /locus_tag="PLO65_09455"
     CDS             complement(4554..4757)
                     /locus_tag="PLO65_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRD28514.1"
                     /translation="MSIDRAVFVFAGFVVLASIALGHFVSPWWLLLGVFAGLNMIQAA
                     FTGFCPAAMVFKKLGARPGAAFH"
     gene            complement(4779..>5080)
                     /locus_tag="PLO65_09460"
     CDS             complement(4779..>5080)
                     /locus_tag="PLO65_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRD28515.1"
                     /translation="HREATRGAAVLVDVWAPWCGPCRAMAPQFEAAAAVLEPGVRLLK
                     LNADAEPATTAELGVSGIPALILYRDGQIVARQAGAMTKDQIVAWTTQALAQAPA"
BASE COUNT          760 a         1681 c         1871 g          768 t
ORIGIN      
        1 gcgagcgtcc agatccgttc gcgcccaccc gccagccggc cggccgccgt cgccgcgacc
       61 gcggtgccct cccccacggc ctgactcggc gccgcgtagt ggcgcttgta gtgatcgtgg
      121 ctggggccga ggtcgtaggt cgtcagcccg agggccggca tggccgcgat ggcccgcatg
      181 aggaaggtct ggccgggcga gaactcgccc aggtcgggat tggtcgaggc cacccatgga
      241 tggtaccagc cgttctgggt cacgccgaag tggcccgcgg cgatccggtc gccggcccac
      301 aggaccaggg tcaggccgcg cagcggaccg gtggcggcga ggaagccggc cagcagccga
      361 tgggtccagt cggggcgcag gaagtcggcc accccggtgc gaatcagctg ctcgcgcttc
      421 cagtccatga tctgttcata gaccgccggt tcgtcgctgg cttcgatgcg cacgggcccc
      481 agttcgcgtt ccatcttgtt gtcgaggcgg cgccagttct tgaagcgctt gggcgagacg
      541 gcgcgcaggc tctcgagata ggcctccggc gagatcgtca gggtgatggc gtagcccgag
      601 gcggcgccgg ccgccgcatc ctggaacatc ccgtgcgggt cgaccaggcc ggtgaagcgg
      661 taggccgaca gccccgccag ctccagcgcc cgcgcgcccg tcaggccgaa atcgggtccg
      721 gcgatcaggc catgatagtc ggacagcggc gcgccaagcg gacgggccag gccgcccggc
      781 cggcggtggt ggggaaggaa tccgacgatg gtctctccgg cgcgatacac cgccacccgg
      841 gcgtcctcgc gcacggcgcc gacggccagg gcgaagtcgg gacccagcaa cgggctgccg
      901 aacgccggct gcgccgcgac cagcgcacgc caggcggcca gctcctgagg cgacaggagg
      961 gaggggtgga tcgacttgac gctcagcatc ggcagcggct cattctgggg acaacccttg
     1021 tcccggtaag ggattttcgc ccccacccca gcaattcgcg ggccgcttca gcgactgaat
     1081 acgtcggcca gaatacgctc cagccagcgc gcatcggtct cgtcgaacgc ggccggtcgg
     1141 tcggaatcga cgtcgaacac cgcgatcagc cgcccgtcgg gtccgaagac cgggaccacc
     1201 acctcgctcc gcgagcggct gtcgcaggcg atgtgtcccg gaaaggcctc cacgtccggc
     1261 accagctggg tctcgccgct ggccgccgcc gcgccgcaga cgccgcggcc gaaggcgatg
     1321 cgcaggcaac cgagcgtgcc ctggtaggga ccgacgacca gttcgtccgt cctgtcggga
     1381 tcgacgacgt agaagccggt ccagaaatag tcgtcgaagc ttgccgccag catcgaggcc
     1441 accgtggcca tccgggcggt caggttcggc tcgcccttga gaacggaggc gatctccgcg
     1501 gcgacctcgg cgtagcgggc ctcgcggtcg gcggacaggg tcaggtcgtt gaaggcttcg
     1561 gccatgtcgg ggatataggg gatcggcggc gaaggagcga gcgatggcgt actggctggt
     1621 gaagtccgaa ccggacgtct tctcgtggga tgacctggtc gccgcgggac cgaagggtga
     1681 gccgtggaac ggcgtgcgca accacaccgc caagctgaac atgatggcca tgcggctcgg
     1741 cgacggggtg ttcttctatc actcccaggc cggcaaggag atcgtcggcg tcgccgaggt
     1801 ggttcgcgag gcctatcccg atccgaccga cgccaagggt gtgttccagt gcgtcgacct
     1861 gcgggccgtc gaaccgctgg cgcggccgtt caccctggcc caggccaagg ccgacccggc
     1921 cctggcggcc atgtcgctgg tgacctcgtt ccggctgtcg gtgcagccgg tgaccgcggc
     1981 cgaatgggcg cacattctgg ccgcggccag gtagcgaaac ggggacgcat gatcggcgtc
     2041 cccgtcccgt ctccacctcg atgcaggcag gggcgacgcg agcgccgccc gccgttctac
     2101 atgcgctcga cgatgatcgc cggggccatg ccgccagcgg cgcacatggt caccaggccg
     2161 tagcgcttgc cggtccgctc gagctcgtcg acgatcgtgc cgatgaggat cgagccggtg
     2221 gcgccgatcg ggtggcccag cgccatggcg ccgccgttga cgttgacctt ggagcggtcg
     2281 agcttcaggt cgcggatgaa cttctcggcc acgaccgaga aggcctcgtt gatttcccac
     2341 aggtcgatgt cctcgaccgt caggccggcc ttggccagga ccttcttcgc cgccggcacc
     2401 ggggcgttga gcatcagggt ggggctgtcg cccatgttgg ccatggccac cacgcgggcg
     2461 cggggcgtca tgccattggc cttggcgtag gccggcgagg ccagcagcag ggccgccgcg
     2521 ccgtccacga cgcccgagct gttgccggcg tggtggaaga atttcaggtc gaggtccgga
     2581 tagaccgcgg tgatcagctt gcggaaggtg gtgccatcct tgtcgagcgg cacgtcggcc
     2641 agggcctcga acgagggctt cagggccgcg aggccttcgg cggtcgtctg cggacggggg
     2701 aactcctcat ggtcgagggc caaggttccg tcttccttgt agacggggat caggctcttg
     2761 gcgaagcggt tctccgcgat ggccacggcg gcgcgctgct ggctgaccag ggcgagggcg
     2821 tcgagggctt cgcggctgat gccttccagg gtggcgatgg agtcggcgca gatgccctgg
     2881 tgcgactggg gatgcctggc gcgcagacgc aggttgccct ggtccatggt caccgggcca
     2941 tcctccggcg agcgccggtt gggcatcgac atcatctcgc aaccgccggc cacgaccatg
     3001 tcggacatgc cggccatgat cgtcgaggcg gcgaagttca cggtggtgat gcccgagcca
     3061 cagaagcggt cgagggtcac gccgctggac ttgatgtcat agccggcgtc gagcgccgac
     3121 atgcggccga ggtcgcccgc ggccgggccc cgctgggagc tggtgcccca gatcacgtcg
     3181 tcgacttccg cggtgttgat cttgttgcgc tcggccagcg ccttgaggac ggtcgcgccg
     3241 agctgctgcg ggtgcatctc ggacagggcg cccttgccgt atttgccgat gccgcgcggg
     3301 gtgcggacgg cgtcgatgat ccaggcttca ctcatggtcg tgttccttcg gaggggatgg
     3361 gtctgaaagt cggaagtcag cggggggtca tgcgaatggc gccgtcgaga cggacgtcct
     3421 cgccgttgaa atagccattg gtgatcatgg tgtgggccag gtgggcgtac tcgttcggat
     3481 cgccgaggcg gcgcgggaat ggcacctggg cggccaggcc ggccttcaca tgctcggggg
     3541 cgcggcccag cagcggggtg tcgaagatgc ccggcaggat ggtgttgacc cggatggcct
     3601 cgttcgagag gtcccgggcg atgaccaggg tcatgccgac gatgccggcc ttggaggcgg
     3661 tgtaggccac ctggccgatc tggccgtcct cggccgccac cgaggcggtg ttgacgatcg
     3721 ccccgcgatc gccgtccggc agcggatcca gcgtcagcat gccggcggcg ctcttggtga
     3781 tgcagcggaa ggtgccgacc aggttgatct gcagaatgcg ctcgaagttg tcgagcgggt
     3841 actccttgac ctcgccggtc tgcttgtcgc gcgagacggt cttggccccg ccgccgaccc
     3901 cggcgcagtt gaccaggatg cgctcctggc cgatcgccgc ccgcgccttg gcgaaggcgg
     3961 cgtcgacctc ctcgccggag gccacgttga ccttgcagaa gacgccgccc agctccctgg
     4021 ccagggcctc gccgagctcc tcgttgaggt cgaagatggc cacccggact ccgtcggcgg
     4081 ccagccgccg ggcggtcgcc gcgccgaggc cggacgcgcc gccggtgatg acggcggaga
     4141 tggttgaatc gagcttcatg atgcgggtct cccgggcgtg ggccgtctgc gcgacgcgac
     4201 gatcaccaca aaccagcgtc ccgttgcgga aggcaagttc aatggccgcg acgcccgccg
     4261 tcgccgcagc ccgggcccgc ggccagaggc tatttggccc ggggcgcctt gcaggtgttg
     4321 atccccagca cggcgtagag cgggcagaag ccgacgatgc ccgtgagcag cgggatcgcg
     4381 cccagatagc cccaggccgt cttcggcccg acgaaggcca gcgacagcac gaccaggccg
     4441 acgatgatgc ggaggacgcg atccaccgcg ccttcattga gtttcatgag tcatctcctg
     4501 cgggtccacg gcccatgccg ggacgggttg gacggcgggc ggacgggccc gtatcagtgg
     4561 aaggccgcgc ccgggcgggc cccgagtttc ttgaacacca tcgccgccgg gcagaagccg
     4621 gtgaaggcgg cctggatcat gttgagcccg gcgaagacgc ccagcagcag ccaccagggg
     4681 ctgacgaaat ggcccagcgc gatgctggcc agaaccacga agccggcgaa gacaaagacg
     4741 gcgcggtcga tagacatgac aatggtctcc tttgcgggtc aggccggggc ctgggcaagc
     4801 gcctgggtcg tccaggcgac gatctggtcc ttggtcatgg ccccggcctg acgcgcgacg
     4861 atctggccgt cgcggtacag gatcagggcc gggatgcccg agacgccgag ttcggcggtg
     4921 gtcgccggtt cggcgtcggc gttgagcttg agcaggcgca ccccgggctc gagcaccgcg
     4981 gcggcggcct cgaactgggg cgccatcgcc cggcagggtc cgcaccaggg ggcccagacg
     5041 tcgaccagca ccgccgcgcc gcgggtcgcc tcgcggtggg
//
LOCUS       DAORNX010000196         5032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12375_length_5032_cov_6.040788, whole genome shotgun sequence.
ACCESSION   DAORNX010000196 DAORNX010000000
VERSION     DAORNX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12375_length_5032_cov_6.040788"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLO65_09465"
     CDS             <1..416
                     /locus_tag="PLO65_09465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRD28516.1"
                     /translation="GQLDEAAPLYERVLALEPRCRVTGVTNPWLLIASHIKPWRSCAT
                     AAERLDGANGLMLTPDVDRLFDRGIIGFSDEGAVLVSPALVEADLAAMGLAGLAARCV
                     GPFTAAQAGYLDWHRRWVFVGGDLRTDSPLEPLME"
     gene            complement(548..1306)
                     /locus_tag="PLO65_09470"
     CDS             complement(548..1306)
                     /locus_tag="PLO65_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD28517.1"
                     /translation="MNPAYRDFEFDLPEALLQRLVQVLDDLEPAPLTQLGLEGVPDAQ
                     GVYQLFKDGVLVYVGKTDSEAGLRRRLSRHAQKILHRIGLSPEQVSYKAVRIYVFTAI
                     DLETQLIRHYAGKGGTAWNNSGFGANDPGRKRDHTRVKSSNFDAIYPIDIDRPLDVSF
                     ADCTSAAQALSRAKLAIPYNLRFESGHARRPHEDLLNAPLSLEDQVFSLRGLLAAVAR
                     SLPAGWQATALPGYVLLYKEQDDTYPDGQVLART"
     gene            complement(1303..2466)
                     /locus_tag="PLO65_09475"
     CDS             complement(1303..2466)
                     /locus_tag="PLO65_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRD28518.1"
                     /translation="MKSIELFAGAGGLGMGLHQAGFHPAKVVEWDRYCCDTIRENQTH
                     EISLVRDWPLVEGDVRHVDFREFEGKVDLVSGGPPCQPFSLGGKHKAYNDARDMFPQA
                     IRAVRETRPRAFVFENVKGLTRTAFRNYFEYIRLQLMHPEIVARADEDWSDHLARLED
                     HEVHGSRSGLHYRLLARVLNAANYGVPQKRERVVFVGFRDDLDLEWHFPTETHSADSL
                     LWDQFGSDSYWDRHRVATADRPIDPAAVARADRLGGRPMTCAWRTVRDAIHDLPDPET
                     QPALAKRIANHRFQPGARSYAGHTGSPLDEPAKTLKAGVHGVPGGENMLTRPDGSVRY
                     FSVRESARLQTFPDSYVFHGSWSETMRQLGNAVPVRLAEVIGKDVARRLQFAA"
     gene            complement(2463..2876)
                     /locus_tag="PLO65_09480"
     CDS             complement(2463..2876)
                     /locus_tag="PLO65_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRD28519.1"
                     /translation="MDTMTPIERSERMSRVRGKGSKAELLVRSLVHRMGYRFRLHGAK
                     LPGKPDLVFQGRRKVIFVHGCFWHRHPDPDCKLARMPKSRQDFWRPKLEGNRARDLRQ
                     IAELQALGWSVLEIWECQLRDPSFLENEIRTFLDS"
     gene            complement(2881..3798)
                     /locus_tag="PLO65_09485"
     CDS             complement(2881..3798)
                     /locus_tag="PLO65_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRD28520.1"
                     /translation="MAKGVFDTKAESGYDDDITRRYHFPTKRNYVEAAEAVVGDWILY
                     REPQRNGGRRAYIAAAFVERIEPDPGRVGHAYAIIGEFIPFDPPVPFAAGGTYWEASL
                     RLIDDPNRIGASLQNKSVRVIDDEDFARIVDAGMAETLAPENAIRVGLGFSEGEPPPF
                     NFDGLLDGSARRVEQILMNKKIRDANFRRQVCQAYDDRCAVTGLRIINGGGRSEVQAA
                     HIWSVGSGGPDVVQNGLALSGTVHWLFDRHLISLTDDYRLLVSHNKVPGELRSLFEKQ
                     LDRIHLPANPKLWPHPGYIQRHREAFHAF"
     gene            complement(3877..4275)
                     /locus_tag="PLO65_09490"
     CDS             complement(3877..4275)
                     /locus_tag="PLO65_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD28521.1"
                     /translation="MTSNPGARFVATGIGVSDLGRSADFYTRVLGMTVQQTFKLDHMD
                     EIMVGYEGKGASLVLMHWTDGSPRNYRDNPIKIVLYVPDATAAVEAIRAEGLEVVREP
                     AASPGMGGALIAFGKDPDGYLVELIQRAAR"
     gene            complement(4349..4573)
                     /locus_tag="PLO65_09495"
     CDS             complement(4349..4573)
                     /locus_tag="PLO65_09495"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRD28522.1"
                     /translation="MTLRYDPEADAVYIKLADGDYDESEEVSPGVILDFTKDGQVMGI
                     EILGASKTLAPGAWSKAAPRDAGQDIQAAE"
     gene            complement(4570..4842)
                     /locus_tag="PLO65_09500"
     CDS             complement(4570..4842)
                     /locus_tag="PLO65_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRD28523.1"
                     /translation="MKPIRWMKHARDNLRDRGLSPAWAEAAIRAPDWTAPDPNDPAVE
                     RRFKVIAEMDGRVIRVPCVETADEIRALTVLLDRGARRALRRKARS"
     gene            complement(4902..>5032)
                     /locus_tag="PLO65_09505"
     CDS             complement(4902..>5032)
                     /locus_tag="PLO65_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28524.1"
                     /translation="GDASRKRKLLDKQKAGKKRMRQFGKVEIPQEAFIAALKMDDN"
BASE COUNT          884 a         1609 c         1607 g          932 t
ORIGIN      
        1 aggggcagct cgacgaggcc gcgccgctgt acgagcgcgt gctggcgctg gagccgcgct
       61 gccgcgtcac gggcgtgacc aatccctggc tgctgatcgc cagccacatc aagccctggc
      121 gcagctgcgc cacggccgcc gagcgcctcg acggcgccaa cggcctgatg ctgaccccgg
      181 acgtcgaccg gctgttcgac cgggggataa tcggattctc ggatgagggg gctgtgctgg
      241 tctcgccggc tctcgtcgag gcggacctgg ccgcgatggg gctggcgggg ctggcggcgc
      301 ggtgcgtggg cccgttcacg gccgcccagg cgggatacct cgactggcat cggcggtggg
      361 tgttcgtggg cggcgatctc cggacggact cccccctgga gcccctgatg gaatgatccg
      421 ctccggaccg ccggcctcgt caaggaccgc ctccggcggc cgcgccgcgg cgggccctgc
      481 gggcccgtcc ctgaccaggc cgacggccgg agcatgctgg ccgtcaagac gtcaggggga
      541 ggggtggcta ggtccgggcg agaacttgac cgtccgggta ggtgtcatct tgctccttat
      601 agagcaacac gtagccagga agagcggtcg cctgccagcc tgccggaaga cttcgggcga
      661 cggcagccaa gagtcccctc aatgagaaaa cctgatcttc tagactcaag ggagcgttga
      721 gcaggtcctc atggggccgc cgtgcatgcc ccgattcaaa ccgaagattg taggggatcg
      781 ccagcttcgc ccggctcaga gcctgcgcgg cggaggtgca atccgcgaac gacacgtcga
      841 gaggacggtc aatgtcgatc gggtagatgg cgtcaaaatt gctcgacttc acacgagtat
      901 ggtcgcgctt tcgtcccgga tcatttgcgc caaaaccgct gttgttccaa gcggtcccgc
      961 ccttgcccgc atagtgccgg atcagttggg tctctaagtc gatcgcagtg aatacgtaga
     1021 tccgaacggc cttgtaggac acctgttcgg gcgagagccc aatgcgatga agaattttct
     1081 gagcatgccg ggaaagccgc ctgcgcaggc cagcttcact atcggtcttg ccgacgtaga
     1141 cgagcactcc atccttgaac aactggtaca cgccttgagc gtcggggacg ccttccaagc
     1201 ccaactgcgt caggggagcc ggctcaagat catccaacac ttggacaaga cgctggagca
     1261 gcgcctcggg caggtcgaat tcgaagtcgc gataagcagg gttcacgcgg cgaactgtaa
     1321 ccgccgcgcg acgtccttgc caatcacctc cgccagacgc accggaacgg cgttgccgag
     1381 ctggcgcatc gtttccgacc aggagccgtg gaacacgtag ctgtcgggga aagtctggag
     1441 ccgcgcgctt tcgcggacgg agaagtatcg aacactgccg tcgggccgcg tcagcatgtt
     1501 ctcgccgccc ggcacgccat gaacgcccgc cttcagcgtc ttggccggct cgtcgagcgg
     1561 actgcctgtg tggccggcgt agctgcgggc tccgggctgg aaccgatggt tggcgatccg
     1621 tttggccagc gctggttgcg tctcggggtc gggcaggtcg tggatggcgt cacgcacggt
     1681 acgccaggcg caggtcatgg gccttccgcc caggcgatcc gcccgcgcca cggcggccgg
     1741 atcaattggg cgatccgccg tggcgaccct atgccggtcc caatagctgt cgctgccgaa
     1801 ttgatcccag agcaggctat cggcgctgtg ggtctcggtc gggaagtgcc attccagatc
     1861 caggtcgtcg cggaagccga cgaacacgac ccgctctcgc ttctgcggaa cgccgtagtt
     1921 ggcggcgttc agcactcgag ccaacagccg gtagtgcagc cccgatcttg agccgtggac
     1981 ctcgtgatcc tcaaggcgcg cgaggtggtc ggaccagtcc tcgtcggcgc gcgcgacgat
     2041 ttctgggtgc atcagttgaa gccggatgta ttcgaaatag ttgcggaacg cggttcgggt
     2101 caggcccttg acgttctcga acacgaaggc ccgcggccga gtctcgcgca cggcgcggat
     2161 cgcctgcggg aacatgtcgc gcgcgtcgtt gtaggccttg tgcttgccgc ctagcgagaa
     2221 cggttggcag ggcgggccgc cagagaccag gtccaccttt ccctcgaact cgcggaagtc
     2281 gacgtggcga acatccccct cgaccaatgg ccaatcgcgc acgagcgaga tttcgtgggt
     2341 ctggttctcg cggatcgtgt cgcagcagta gcgatcccat tccacgacct tggccggatg
     2401 gaagcccgcc tgatgcaggc ccatgccgag cccgccggcg cccgcgaaga gttcgatcga
     2461 cttcatgagt ccaagaatgt tctaatttcg ttctctagaa aagaggggtc tcgaagttgg
     2521 cactcccaaa tctccagaac cgaccatccg agggcctgga gctcggcgat ctggcgcagg
     2581 tcgcgcgcgc gattcccctc cagcttcggc cgccagaagt cctgccgtga tttgggcatc
     2641 cgcgccagct tgcagtcggg gtccgggtgc cgatgccaga agcagccgtg cacgaagatc
     2701 accttccgcc gcccctgaaa cacgaggtcc ggcttgccgg gcagtttcgc gccgtggaga
     2761 cggaagcggt agcccatgcg gtggacgagc gaacggacga gaagctcagc cttgctgccc
     2821 ttgccgcgaa ctcgcgacat ccgctcgctg cgttcaatgg gagtcatagt gtccatggaa
     2881 tcagaatgcg tggaatgctt ctcgatgccg ttgaatgtac ccgggatggg gccacagttt
     2941 gggatttgca ggcaggtgga ttcgatccaa ctgcttttca aataggcttc tcagctctcc
     3001 agggaccttg ttatgtgaaa cgagcaaccg atagtcatcg gtcagggaaa tcaggtgtcg
     3061 atcgaacaac caatgcacgg ttcccgaaag cgcgagccca ttttggacga cgtcgggtcc
     3121 gccgctgcct acggaccaaa tatgtgccgc ctggacctct gagcggccac cgccgttgat
     3181 gatccgcagc ccggtcacgg cacatcgatc atcgtaggct tggcatactt gtcggcgaaa
     3241 gttcgcgtcc cgaatcttct tgttcatcag gatttgctcg acgcgccgag cgcttccgtc
     3301 cagcagccca tcgaaattga acggcggcgg ttcgccttcg ctgaagccca gacccacgcg
     3361 gatcgcgttc tcgggggcaa gcgtttcggc catgcccgcg tctacgatgc gcgcgaaatc
     3421 ctcgtcgtcg atcacccgaa cggatttgtt ctgtaggcta gcgccgatgc ggtttggatc
     3481 atcaatcaaa cgcagcgatg cttcccaata ggtcccgccg gcggcaaacg gcactggtgg
     3541 atcgaaggga atgaactcgc cgatgatcgc ataagcgtgg cctacgcgcc caggatcagg
     3601 ctcaatgcgt tcaacaaagg cagcggcaat atacgcccgg cgaccaccgt ttcgttgcgg
     3661 ctctcgatag agaatccaat cgccgacgac cgcttctgca gcctcgacat agtttcgctt
     3721 cgtcgggaaa tgatacctgc gcgtgatgtc gtcgtcgtag ccagattcag ctttggtatc
     3781 gaagacaccc tttgccaatg cctcgttccc aacaacgagc ccctccagac gagctgctca
     3841 tcatctcatc atgaagagat tccggcgagc gtccatctac cgcgccgccc tctggatcaa
     3901 ctccaccaga tacccgtccg ggtccttgcc gaacgcgatc agggcgccgc ccatgccggg
     3961 cgaagccgcc ggttcgcgca cgacctccag gccctcggcc cggatcgcct cgaccgccgc
     4021 cgtcgcgtcg ggcacgtaga ggacgatctt gatcgggttg tcgcgatagt tgcgcggcga
     4081 gccgtcggtc cagtgcatca gcaccagtga cgcgcccttg ccctcatagc cgaccatgat
     4141 ctcgtccata tggtcgagct tgaaggtctg ctgcaccgtc atcccgagca cgcgggtgta
     4201 gaagtcggcc gagcggccca ggtcggacac gccgatgccg gtagcgacga agcgggcgcc
     4261 cgggttgctg gtcatggtgt gcgttccccc ttttgcggag agggtagggc ggggtgaagc
     4321 gcaattgaac ccttcgccgg ggcgggggct actccgccgc ctgaatgtcc tggcctgcat
     4381 cccgcggcgc ggcctttgac caggcgccgg gcgcgagcgt cttgctggcg ccaaggatct
     4441 cgatgcccat gacctgaccg tccttggtga aatcgaggat cacgccgggc gagacttcct
     4501 cactctcgtc gtagtcgccg tccgccagct tgatgtagac ggcgtcggct tcggggtcgt
     4561 agcgcagggt catgatcgcg cctttcgtcg gagcgcccgc cgggcgccgc gatccaacaa
     4621 taccgtaagc gcccggattt cgtcagcggt ttcaacgcag ggcacgcgga tcacgcgtcc
     4681 gtccatctcg gcgatgacct tgaaccggcg ctccacggcg gggtcgttcg ggtcgggcgc
     4741 ggtccagtcc ggtgcgcgga tcgccgcttc ggcccacgcc ggcgacagcc cgcgatcacg
     4801 cagattgtcc cgcgcgtgct tcatccagcg gatcggcttc accgtcgaag tgaagcacca
     4861 ggatcgctaa aaatcaacca caaggtgcaa attgtcgaag ttcaattatc gtccatcttc
     4921 agcgcggcga taaacgcttc ctgcgggatc tcgaccttgc cgaactggcg catccgcttc
     4981 ttgccggcct tctgcttgtc cagcagcttg cgcttgcggc tggcgtcgcc gc
//
LOCUS       DAORNX010000197         5025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12396_length_5025_cov_7.195372, whole genome shotgun sequence.
ACCESSION   DAORNX010000197 DAORNX010000000
VERSION     DAORNX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12396_length_5025_cov_7.195372"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLO65_09510"
     CDS             <1..783
                     /locus_tag="PLO65_09510"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRD28525.1"
                     /translation="DRIDEDLKGLHCLRHGRFGLFSFDGRDHGWRDGRPLRGWVEARL
                     AETGVPAGAARIRLLTFPRVLGFVFNPISLFYVESAVGALEAVIYEVNSTFGQTHAYV
                     APARGMGRQRQVAEKRLFVSPFYGVEGTYRFDVSPPGSTLDLSIVKATDGHPEFTATL
                     KLQRRPMTDRRLLALFVRMPLMTLKVVAAIHWEALRLVLKGIPLISRPPAPDRGASRA
                     TLTSRATGENDVTLSGTERSADDVHGPDTRARRTGRRAAGPA"
     gene            719..1960
                     /locus_tag="PLO65_09515"
     CDS             719..1960
                     /locus_tag="PLO65_09515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRD28526.1"
                     /translation="MTFTDLTRGPGGLAAEPPALRSAPAVFRTAVRICADNWRHGRLT
                     WVLPDGTEIPCRGPEPGPEARLVVRDYRFIRRCLAAGSIGFAEGFMADEWDTPDLSAL
                     LTGFALNFDRLQALVQGNPVVRAINFISHLMRRNDRRGSRKNIHAHYDLGNHFYARWL
                     DSSMTYSSAVFERPGEPLAEAQKHKYAALARSIGLQPGQSVLEIGCGWGGFAEFAAAE
                     VGAKVTGITISREQYDFAKKRLFEQGLAEKADIRLVDYRDVDGRFDRVASIEMFEAVG
                     EQYWPTYFAKIRDVLSPGGRAGLQIITIKDELFDVYRRHADFIQKYIFPGGMLPSEAA
                     LRRQVDSAGLVWDDAPRYGQHYADTLAEWGHRFEAAWDDIRRLGFDERFRKMWRFYLS
                     YCEAGFRTGRTNVIQLALSKV"
     gene            2206..3219
                     /locus_tag="PLO65_09520"
     CDS             2206..3219
                     /locus_tag="PLO65_09520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRD28527.1"
                     /translation="MSAAASVLDLIGNTPLIRLRAASDATGCEILGKAEFLNPGQSVK
                     DRAALGIIRDAEAKGLLRPGGRIVEGTAGNTGIGLAMVASALGYNTTIVIPRTQSQEK
                     KDAIRLAGADLVEVDAVPYSNPDNYVRWSGRLAEELNRNEPKGAIWANQFDNVANRRA
                     HEATTGPEIWDQTGGEVDGFICAVGSGGTLAGVAAALRARNPDVAIGLADPHGAALYS
                     YFTTGELKAEGSSITEGIGQGRITANLEGLAVDHAFRIADEEMLRVIFDLAEHEGLVL
                     GGSSGVNVAGAMRLARALGPGHTIVTVLCDYGSRYQSRLFNPTFLRERGLPVPGWLQG
                     PDA"
     gene            3219..4064
                     /gene="sseA"
                     /locus_tag="PLO65_09525"
     CDS             3219..4064
                     /gene="sseA"
                     /locus_tag="PLO65_09525"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HRD28528.1"
                     /translation="MSDPLVSTVWLADHLQAPDVRIIDASWYFPQEGRDARAEYALAH
                     IPGAVFFDIDDIADDATDLPHMLPSGVKFASRVRKLGLGDGSRMVVYDQLGLRSAARV
                     WWTFRAMGHLDVVVLDGGLPKWIAEGRPLSDIPSPPRERHFTPRYSADLVRDLNQVKK
                     ALETGREQLVDARPAGRFTGAAPEPRPGLRGGHMPGARNVPASDLIAPDGTLKKASQL
                     EDIFEKAGVDINRPVITTCGSGITAAILALALARLGKDRAAVYDGSWAEWGARDDVPV
                     VTGPA"
     gene            complement(4061..>5025)
                     /locus_tag="PLO65_09530"
     CDS             complement(4061..>5025)
                     /locus_tag="PLO65_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD28529.1"
                     /translation="ASRTLAAVKARGAVRCGVNPGLSGFAFADTQGRWRGFDVDFCRA
                     LAAAVLGDASRVVFVPLTNEARFVALKAGRVDLLSRNTSWTFTRDAGEGVDFAGVSYY
                     DGQGFLAPREFELQSAAELSGAKVCVQSGSTSELNLRDWFTARSINWMPVLSPTLERA
                     RQNYEQGICDVLSADISALASARATLADQNAHVLLPDVISKEPLGPAVRQGDPGWTDV
                     VRWTLNALVLAEELGVTADNAEQMRKQSASPEARRLLGAEPGYGRMLGLDDAWAYRAI
                     RAAGAYNEIFDRNLGRDSALKLERGQNALWNAARPGLLYAPPMR"
BASE COUNT          779 a         1784 c         1679 g          783 t
ORIGIN      
        1 gaccggatcg acgaggacct gaagggcctt cactgcctgc gccacggccg gttcggcctg
       61 ttctcgttcg acggtcgcga ccatggctgg cgcgacggcc ggcccctgcg cggctgggtc
      121 gaggcccggc tggccgagac cggtgttccc gccggcgcgg cccgcatccg gctgctgacc
      181 tttccccgcg tgctgggctt cgtgttcaac ccgatctcgt tgttctacgt cgagtccgcg
      241 gtcggcgcgc tcgaggcggt gatctacgag gtcaacagca ccttcggtca gacccatgcc
      301 tatgtcgcgc ccgcccgcgg catgggccgg cagcgccagg tggcggagaa gcgcctgttc
      361 gtctcgccct tctacggcgt cgaggggacc taccgcttcg acgtgtcgcc gcccggatcg
      421 acgctggacc tgtccatcgt caaggcgacg gacggccatc ctgagtttac ggcgacgctc
      481 aaactccagc gtcgaccgat gaccgaccgc cggttgctgg cgcttttcgt ccgcatgccg
      541 ctgatgaccc tgaaggttgt tgcggcaatc cactgggagg cgctgcgcct tgtattgaaa
      601 gggattccgc tgatctcccg accgccggcg cccgaccgcg gcgcgagccg cgccaccctc
      661 acctctcgcg cgacgggaga aaacgatgtt acgctcagcg ggacggaaag gagcgccgat
      721 gacgttcacg gacctgacac gcgggccagg cggactggcc gccgagccgc cggccctgcg
      781 tagcgcgccc gcggtgttcc ggaccgccgt ccggatctgc gccgacaact ggcggcatgg
      841 ccgcctgacc tgggtgcttc ccgacggaac cgaaatccct tgccggggcc cggaacccgg
      901 gcctgaagcc cgcctcgtcg tccgcgacta ccggttcatt cgccgttgcc tggccgccgg
      961 ttccatcggc ttcgccgagg gcttcatggc cgacgaatgg gacacgccgg acctgtcggc
     1021 cctgctgacc ggattcgccc tgaacttcga ccggctgcag gccttggtgc agggcaatcc
     1081 ggtggtccgg gcgatcaact tcatcagcca tctgatgcga cgcaacgatc gccgcgggtc
     1141 acgcaagaac attcacgccc actacgacct gggcaaccac ttctacgccc gctggctcga
     1201 ctcgagcatg acctacagct cggccgtctt tgaacggccc ggcgaaccgc tggcggaggc
     1261 ccagaagcac aagtatgccg ccctggcccg gtcgatcggc ctgcagcccg gccagagcgt
     1321 gctcgagatc ggctgcggct ggggcggctt cgccgaattc gccgcggccg aggtcggggc
     1381 gaaggtgact ggcattacca tttcgcgcga acagtatgat ttcgcgaaga aacggctgtt
     1441 cgagcagggg ctggccgaga aggccgacat acggcttgtc gactatcggg acgtcgatgg
     1501 ccgcttcgac cgcgtcgcct cgatcgagat gttcgaggcg gtcggagagc agtactggcc
     1561 gacctatttc gccaagatcc gcgatgtcct ctcgccaggc ggccgcgccg gcctgcagat
     1621 catcaccatc aaggacgagc tgttcgacgt ctatcgccgc cacgccgact tcatccagaa
     1681 gtacattttc ccgggcggca tgctgcccag cgaggcggcg cttcggcggc aggtcgacag
     1741 cgccggcctg gtctgggacg acgcgccgcg ctacggccag cactacgccg acacccttgc
     1801 cgaatggggt caccgcttcg aggccgcctg ggacgacatc cgccggctcg gcttcgacga
     1861 gcgcttccgc aagatgtggc gcttctacct cagctactgc gaagccggct ttcgcaccgg
     1921 ccggaccaat gtgatccagc tggcgctgtc gaaggtctga cgggcgccga gggcgaacgg
     1981 cagggcgtag ggagtagggc ggcgaagtcg ctgggcagtc gcgcgccacc agttcctggc
     2041 tgcctggccc cggggcgctg gactactccc tactccctac tccctactcc ctatccctca
     2101 agccctcccc agggccgcta tcagatattc tggggttgcg atcgacgcgg ggcgcatgat
     2161 gtaggctggg tcatcgggcc gctcgtggcc gtcggaatcc atgccatgtc cgctgccgcc
     2221 agcgtcctgg acctgatcgg aaacactccg ctgatccggc tgagggccgc cagcgacgcc
     2281 accggctgcg agattctcgg caaggccgag ttcctcaatc ccggccagtc ggtcaaggac
     2341 cgggccgccc tgggcatcat ccgcgacgcc gaggcgaagg gacttctccg gcccgggggc
     2401 cgcatcgtcg agggcacggc cggcaacacc ggcatcggcc tggcgatggt cgccagcgcc
     2461 ctgggctaca acacaaccat cgtcatcccg cgcacccaga gccaggagaa gaaggacgcc
     2521 atccgcctgg ccggcgccga tctggtcgag gtcgacgcgg tcccctactc caatcccgac
     2581 aactacgtcc gctggtccgg ccgactggcc gaagagctga accgcaacga accaaagggc
     2641 gcgatctggg ccaaccagtt cgacaatgtg gccaaccgcc gcgcgcacga ggcgacaacc
     2701 ggacccgaga tctgggacca gaccggcgga gaggtcgacg gcttcatctg cgcggtcggc
     2761 tcgggtggca ccctggccgg cgtggccgcg gccctgcggg cgcgcaatcc cgacgtggcc
     2821 atcggcctgg ccgaccctca cggcgcggcg ctctacagct acttcaccac cggcgagctg
     2881 aaggccgagg gctcgtcgat caccgaaggc atcggccagg gccggatcac cgccaatctc
     2941 gagggcctgg ccgttgatca cgccttccgc atcgccgacg aggagatgtt gcgggtgatc
     3001 ttcgacctgg ccgagcacga gggcctggtg ctgggcggct cgtcgggagt caatgtcgcc
     3061 ggcgccatgc gactggcccg cgcactgggc cccggccaca ccattgtgac ggtgttgtgc
     3121 gactacggat cgcgctatca gagcagactg ttcaatccga cgttcctcag ggagcgcggc
     3181 ctgccggtcc ccggctggct ccagggaccc gatgcctgat gtctgacccg ctcgtctcca
     3241 ccgtctggct cgccgaccat ctccaggcgc ccgatgtgag gatcatcgac gccagctggt
     3301 atttcccgca ggaaggccgc gacgccaggg cggagtacgc cctggcccac attccgggcg
     3361 cggtgttctt cgacatcgac gacatcgccg atgacgccac agacctgccg cacatgctgc
     3421 ccagcggggt caagttcgcc tcgcgggtcc gcaagctggg cctgggcgac gggtcccgca
     3481 tggtggtcta cgaccagctc ggcctgcgct cggcggcgcg cgtctggtgg acgttcaggg
     3541 ccatgggcca cctcgacgtg gtcgtgctcg acggcgggct gcccaagtgg atcgccgagg
     3601 ggcggccgct gtccgacatt ccctcaccgc cgcgggagcg tcacttcacg ccgcgctaca
     3661 gcgccgatct cgtgcgagac ctgaaccagg tgaagaaggc cctcgagacg ggccgcgagc
     3721 agctggtcga cgcccggccc gccggccgct tcaccggggc ggcgcccgag ccgcgccccg
     3781 gcctgcgtgg cggccatatg cccggcgccc gcaatgtgcc ggccagcgat ctgatcgccc
     3841 ccgacggtac gttgaaaaag gcgtcgcaac ttgaagatat ctttgagaaa gccggcgtgg
     3901 acatcaaccg gccagtgatc accacctgcg gctcgggcat taccgccgcc attctcgccc
     3961 tggcccttgc ccgcctcggc aaggaccggg ccgcggtcta cgacggttcc tgggccgaat
     4021 ggggtgcgcg cgacgacgtc ccggtggtca ccgggcccgc ctaacgcatc ggcggcgcgt
     4081 acagcagccc gggtcgggcg gcgttccaca gggcgttctg gccgcgctcg agcttgagcg
     4141 ccgagtcccg gcccagattg cgatcgaata tctcattgta ggcgcccgcg gcccggatcg
     4201 cgcgataggc ccaggcgtcg tcgagaccga gcatccggcc gtagcccggc tccgccccca
     4261 gcaggcggcg cgcctcgggc gaggcggact gcttgcgcat ctgttcggcg ttgtcggccg
     4321 ttacgcccag ctcctcggcc aggaccaggg cgttgagcgt ccagcgcacc acgtcggtcc
     4381 agccgggatc gccctggcgc accgctggtc ccaacggctc ctttgaaatg acgtcgggca
     4441 gcagcacatg ggcgttctga tcggccaggg tggctcgggc cgaggccagg gccgagatgt
     4501 cggctgacag gacatcgcag atgccctgct cgtagttctg ccgcgcccgc tccagcgtcg
     4561 gcgacaagac cggcatccag ttgatcgacc gggccgtgaa ccagtcgcgc aggttcagtt
     4621 cgctggtcga gcccgactgg acgcagacct tcgccccgga tagttccgcg gcgctctgca
     4681 gctcgaactc gcgtggagcc aggaagccct ggccgtcata gtagctgacg ccggcgaaat
     4741 cgacgccctc gcccgcgtcg cgggtgaagg tccagctggt gttgcgcgac agcagatcga
     4801 cccgtccggc cttgagggcg acaaaccgcg cctcattggt caggggcacg aacaccaccc
     4861 ggctggcgtc gccgagcacc gccgccgcca gcgcccggca gaagtcgacg tcaaacccgc
     4921 gccatcggcc ctgggtgtcg gcgaaggcga agccggacag gcccgggttc acgccgcacc
     4981 ggacggcgcc gcgcgccttg accgccgcga gagtccggct ggccg
//
LOCUS       DAORNX010000198         5008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12465_length_5008_cov_5.676963, whole genome shotgun sequence.
ACCESSION   DAORNX010000198 DAORNX010000000
VERSION     DAORNX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12465_length_5008_cov_5.676963"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="queE"
                     /locus_tag="PLO65_09535"
     CDS             <1..525
                     /gene="queE"
                     /locus_tag="PLO65_09535"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HRD28530.1"
                     /translation="REQDRARAVCTFCDTDFIGTDGEGGGKFATPAALAAAIDAAWTG
                     PGHDKLVVCTGGEPLLQLDPPLIAALRGRGFMIAVETNGTLAAPPGIDWICVSPKADA
                     PVVQTTGQELKLVYPQPGVDPARFADMAFERFYLQPMDSADREANTAAAVAYCLAHPR
                     WRLSVQTHKYLGLP"
     gene            complement(721..933)
                     /locus_tag="PLO65_09540"
     CDS             complement(721..933)
                     /locus_tag="PLO65_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28531.1"
                     /translation="MVPRFIPALVLLVAAVGLQGCVATVVGATAGAVVGVGAAAVVTT
                     AKVGGAVVGAAIPDGDKKDGKKKRDR"
     gene            complement(992..1501)
                     /locus_tag="PLO65_09545"
     CDS             complement(992..1501)
                     /locus_tag="PLO65_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28532.1"
                     /translation="MRFELIKILLVDDNHHMRVLLTEILRAIGVRHVFEASDGAEALQ
                     LMRTHQVDVVMTDLAMQPLDGIDFVRLLRNSPDSPNQMCPVIMITGHSTSRRVGEARD
                     AGVNEFLAKPVTARGVIERIGEIVEHPRPFIRTGDYFGPERRRKNEAHFAGPWRRSTD
                     PARDEPPAA"
     gene            complement(1654..2349)
                     /locus_tag="PLO65_09550"
     CDS             complement(1654..2349)
                     /locus_tag="PLO65_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRD28533.1"
                     /translation="MIDRRPFEQLGHADHGWLNARHHFSFANWYDPARMGWGALRVWN
                     DDEIAPKSGFPPHPHNDMEIITYVRTGAITHQDSLGNKGRTGAGDVQVMSAGSGIRHA
                     EYNLEDETTTLFQLWIETRERGAPPSWGQKPFPKADRSGKFVALASGFGDEGALPIRT
                     DARVLGATLKAGESTTYPLGDGRHGYLVAATGQVDVNGVTLNPRDGAAIRDEPLLTIV
                     AREDAELVLVDAA"
     gene            2509..2997
                     /locus_tag="PLO65_09555"
     CDS             2509..2997
                     /locus_tag="PLO65_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRD28534.1"
                     /translation="MQTTPDLDDGRQDFDFLVGRWTIRHRRLKARLAGCADWVEFDGT
                     CAMRKVLGGLGNMDDNGLELPDGPYAAVTLRRFDPALRRWSIWWLDARHPDIEPPVHG
                     RFEGGVGEFLGDDVFDGRPIKVRFLWSAITPTSCEWRQAFSEDGGKTWETNWEMAFTR
                     AA"
     gene            complement(3400..4074)
                     /locus_tag="PLO65_09560"
     CDS             complement(3400..4074)
                     /locus_tag="PLO65_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28535.1"
                     /translation="MTPSALLARLSAQEDSYVERKSQGIKPQDIRKAVTAFANSLPDD
                     QHGIIFVGIGDKGSIEGCDNPDAVQKRVREAADECYPPIAPQCFALKVEGKDVVAIVV
                     SASGDRPHFAGPAYVRQGSESVKASKRLYEEMIDSRHSTVSAIVRLKDRPITVYGLGH
                     RLGKLERVAANYLETAECRVMACDRHTVTLQRVSDGWNFYEAVNRIELSTDGKMKRPA
                     LIVKGL"
     gene            complement(4174..>5008)
                     /gene="aroC"
                     /locus_tag="PLO65_09565"
     CDS             complement(4174..>5008)
                     /gene="aroC"
                     /locus_tag="PLO65_09565"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRD28536.1"
                     /translation="RSKDYSEIARAFRPGHADWPYFAKYGVRDYRGGGRSSARETAAR
                     VAAGAVARKVIAGVTVRGALVQIGPHAVDPDRWDWAQTRENGFWCPDAGMVPVWEEHL
                     EKIRKAGSSTGAVVEVEATGVPAGWGAPLYGKLDAELAAAMMSINAAKGVEIGAGFAS
                     AALTGEENADQMRMGNDGVSFLSNKAGGVLGGISSGQPVVVRVAFKPTSSILTPRETV
                     NEAGEDVELRTKGRHDPCVGIRAVPVVEAMMACVLADAFLRHRGQTGGGAFAPGGDGR
                     G"
BASE COUNT          774 a         1715 c         1683 g          836 t
ORIGIN      
        1 cgcgagcagg accgggccag ggccgtctgc accttctgcg acacggattt catcggcacg
       61 gacggcgagg gcggcggcaa gttcgccacg ccggcggcgc tggccgccgc gatcgacgcc
      121 gcctggaccg gccccggcca cgacaagctg gtcgtctgca cgggcggcga gcccctgctg
      181 cagctcgatc cgccgctgat cgcggcgctg cgcgggcggg gcttcatgat cgcggtggag
      241 accaacggaa ccctggcggc cccgccgggg atcgactgga tctgcgtcag ccccaaggct
      301 gacgcgccgg tggtccagac caccgggcag gagctcaagc tggtctatcc ccagccgggc
      361 gtcgatccgg cgcgcttcgc cgacatggcc ttcgaacggt tctatctgca gcccatggac
      421 tctgccgacc gcgaggccaa cacggcggcg gcggtggcct attgcctggc ccatccgcgc
      481 tggcggctga gcgtgcagac gcacaagtat ctcggcctgc cctgaggcgt ccgggtctcg
      541 ggccaatcga cgttcagggc gcgggagccg ccaagggctc cgatggttcc cgcggaggcg
      601 ggaacccaag cggtgtttcg ggtcctgacg cgccgtttcg gcggcgacca aggcttgggt
      661 ccccgccttc gcggggacaa tcggtaggaa agcggccccg gatcgtgatg tcgattggcc
      721 ttaacggtcc cgcttcttct tgccgtcctt cttgtcgccg tcgggaatcg cggcgccgac
      781 caccgcgccg ccgaccttgg cggtggtgac cacggcggcc gcgccgacgc cgacgacggc
      841 gccggccgtg gcgcccacga ccgtagccac acagccctgc aggccgacgg cggcgaccag
      901 caggacaagg gcgggaatga agcgcgggac catggacgac ctccggtgac cgacatccgg
      961 taacggacaa tcgtcgcgga aagatgaccg gtcaggcggc cggcggctcg tcgcgggccg
     1021 gatcggtgct gcgccgccag ggtccggcga aatgagcctc gttcttgcgc cgtcgctccg
     1081 ggccgaaata gtcgccggtg cggatgaacg ggcggggatg ctcgacgatc tcgccgatgc
     1141 gctcgatcac gccgcgggcc gtgacgggtt tggcgaggaa ctcgttgacc ccggcgtcgc
     1201 gcgcctcgcc gacccggcgc gaggtcgaat ggccggtgat catgatcacc ggacacatct
     1261 ggttggggct gtcgggcgaa ttgcgcagca gccggacgaa gtcgatgccg tccagcggct
     1321 gcatcgccag gtcggtcatc accacgtcga cctggtgggt gcgcatcagc tgcagcgcct
     1381 cggccccgtc gctggcctcg aacacgtgcc gcacgccgat cgcgcgcagg atctcggtca
     1441 acagcacccg catgtggtgg ttgtcatcga ccagcaggat cttgatcagt tcgaagcgca
     1501 cgggctgacg gctgttccag gccccggtcg gtagtcatgc gcggaatcgc gccggccccg
     1561 atccgggatt tgcgactcgt aggagccgag ttcttgcccg gtcaaactaa aaaagtgaac
     1621 aggttcgacg acttgccccg gcatgggcgg cgatcaggcg gcgtcgacca gcaccagttc
     1681 ggcgtcctcc cgcgccacga tggtcagtaa cggctcgtcg cggatcgccg cgccgtcgcg
     1741 cgggttgagg gtcacgccat tgacgtccac ctgaccggtc gcggccacca gatagccgtg
     1801 gcgaccatcg cccaggggat aggtcgtcga ctcgccggcc ttcagcgtcg cccccagaac
     1861 gcgcgcgtcg gtgcggatcg gcagggcgcc ctcgtcgccg aagccgctgg ccagggccac
     1921 gaacttcccc gaccggtcgg ccttcggaaa cggcttctgg ccccaggacg gcggcgcgcc
     1981 gcgctcacgc gtctcgatcc acagctggaa cagcgtcgtg gtctcgtcct cgagattgta
     2041 ttccgcgtgg cggatgccgc tcccggcgct catcacctgc acgtcgccgg cgccggtgcg
     2101 gcccttgttg cccaggctgt cctggtgggt gatagcgccg gtgcggacat aggtgatgat
     2161 ctccatgtcg ttgtgcgggt gcggcggaaa gccggacttg ggcgcgatct cgtcgtcgtt
     2221 ccacacgcgc agggcgcccc agcccatgcg ggcgggatcg taccagttgg cgaaactgaa
     2281 atggtgccgg gcgttgagcc agccgtggtc ggcgtggccg agctgctcga aaggcctgcg
     2341 gtcaatcatg ggtcaaatct ccctttcgcg gggagagata ggggcggggc cggataattg
     2401 caactgtctc ggtgtcagaa tgccgccgcc gggcgccatg ctgacaggtc ctggcgaggg
     2461 cgatggctat gggtccccaa cgcatcagcg cggcaaggga tcacggccat gcagaccacc
     2521 cccgacctcg atgacggccg ccaggacttc gacttcctgg tcggccgctg gaccatccgt
     2581 caccggcggc tgaaggcccg gctggcgggc tgcgcggact gggtcgaatt cgacgggacc
     2641 tgcgcgatgc ggaaggtgct cggcgggctc ggcaacatgg acgacaacgg gctggagctg
     2701 ccggatggcc cctacgcggc ggtcacgctg cgccggttcg acccggccct tcgccgctgg
     2761 tcgatctggt ggctggacgc ccgccatccg gacatcgagc cgccggtcca tggccggttc
     2821 gagggcggcg tcggcgaatt cctcggcgac gacgtcttcg acggccgccc gatcaaggtc
     2881 cgcttcctct ggtcggccat caccccgacc agctgcgaat ggcgccaggc cttctcggag
     2941 gacggcggga agacgtggga aaccaactgg gagatggcgt tcacgcgggc ggcgtagggg
     3001 aaggggcagg gtcaagctgt ccctgctgcc cttgggcccc acccctgtca tcccggacgg
     3061 ccgtctcgcc gatccgggac ccagaacaaa gtccagccga atctgggtcc cggctctccg
     3121 ctccgctgcg gccgggatga cagtttgaaa ggcaggcaga atggccacaa ccgaccctga
     3181 tctcatggcg agagtttcgc cggtctcccc agcctgctca agggcgcttc gcgtcgcgga
     3241 gcgacaggcc cgcagggcct gcccttgacc aggctggaga ggccggcacg ctggcccgca
     3301 agagatcgac ggcggggtgt cgaccgccgt ctggtcggag ggtcgagacg tggccccggg
     3361 ggtgggaagc cgccaatccc cctgactccg actcttgttc taaaggcctt tgacgatcag
     3421 cgcgggacgc ttcatttttc cgtcggtact gagttcaatt cggttcactg cctcgtagaa
     3481 gttccaaccg tcgcttaccc tttgaagagt gactgtgtgt cggtcacacg ccatcactct
     3541 gcattcagcc gtttccaggt aattggccgc gacgcgctca agcttcccca gtcgatgccc
     3601 taacccgtat actgtgattg gccggtcctt gagccgcaca attgctgata ctgtactgtg
     3661 tcgagagtct atcatctctt cgtagagacg ctttgacgcc ttcacgctct ctgagccttg
     3721 ccgcacgtag gccgggcctg cgaagtgtgg cctgtcacca ctggcgctga cgactatggc
     3781 gacgacgtcc tttccttcca ccttcagggc aaaacactgc ggagcgatag ggggatagca
     3841 ctcgtcggca gcttcccgta ctcgcttctg cactgcgtca gggttatcgc atccttcgat
     3901 gctgccttta tcaccaatac cgacaaagat gatgccatgc tggtcgtcgg gcaatgagtt
     3961 cgcgaaggcc gttaccgcct tacgaatgtc ttggggcttt atgccttggg acttgcgctc
     4021 gacataacta tcttcttggg cgctcaagcg cgccaataag gcactcggcg tcatttggcc
     4081 ctcattaggc gttgtagcta aacataccct aatttagccg cgcacgaatg tccggcacgg
     4141 gtcgcaggcc aacccgtcct cgccggcccc gctctacccc cgcccgtcgc cccccggcgc
     4201 gaacgccccg ccacccgtct ggccgcggtg ccgcaggaac gcgtccgcca gcacacaggc
     4261 catcatcgcc tcgaccaccg gcacggcgcg gatacccaca cacgggtcat gccggccctt
     4321 cgtccgcagt tcgacgtcct cgccggcctc gttgacggtc tcgcgcgggg tgaggatcga
     4381 ggaggtcggc ttgaaggcca cccgcaccac cacgggctgg ccggaggaga tgccgcccag
     4441 cacgccgccg gccttgttcg acagaaagct gacgccgtca ttgcccatgc gcatctggtc
     4501 ggcgttctcc tcgccggtca gggcggcgga ggcgaacccg gcgccgatct ccacgccctt
     4561 ggcggcgttg atcgacatca tcgcggcggc cagttcggcg tcgagcttgc cgtacagcgg
     4621 cgcgccccag ccagcgggga cgcccgtggc ctcgacctcg acgacggcgc cggttgacga
     4681 gccggccttg cggatctttt ccaggtgttc ttcccacacc ggaaccatgc cggcgtcggg
     4741 acaccagaag ccgttctcgc gggtctgggc ccagtcccag cggtcggggt cgaccgcgtg
     4801 cggaccaatc tgcaccaggg ccccgcgcac ggtgacgccg gcgatcacct tgcgcgccac
     4861 ggccccggcg gccacccgcg cggccgtctc gcgggccgag gaccggcccc cgccgcgata
     4921 gtcgcgcacg ccgtacttgg cgaaataggg ccagtcggca tggccgggcc ggaaggcccg
     4981 ggcgatctcg gaatagtcct tcgaccgc
//
LOCUS       DAORNX010000199         5004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12478_length_5004_cov_5.765811, whole genome shotgun sequence.
ACCESSION   DAORNX010000199 DAORNX010000000
VERSION     DAORNX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12478_length_5004_cov_5.765811"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLO65_09570"
     CDS             complement(<1..623)
                     /locus_tag="PLO65_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD28537.1"
                     /translation="MQATAAVARAPGADFTLEAVELDEPRADEILVRVAAVGVCHTDL
                     VAREGAMPFSLPAVLGHEGAGVVERVGAAVTKVAPGDRVAMTFRSCGQCDRCRSGDPA
                     YCYSMPALNYIGMRPDGSKAIRQGETAVSSNFFGQSSFATYALAYERNVLKLPDDLPF
                     PLAAPLGCGVQTGAGGVMLALACPTGSSLLITGGGAVGLSAVMGAAI"
     gene            complement(635..1720)
                     /locus_tag="PLO65_09575"
     CDS             complement(635..1720)
                     /locus_tag="PLO65_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD28538.1"
                     /translation="MTEPSIAATVDEIDPDWMTAALRASGALPRGRVTGLTVTPVGNG
                     LVGDSFRLALTYDGAPPSAPASVVGKFAAADPASRASGAAFNLYGREVGFYRELASTV
                     GIITPKTHFAGIDPQTAAFTLIFEDFGPARAGDQLTGCSVADARTAMLEAAALHAPRW
                     NDPALRDIEWLAAGADMYPAIIAALPGVIAAYRERYDGVLEADYMALVDRLPQIMGLF
                     QGAAEGPFTIQHGDFRLDNILFDIQGGTRRMGTLDWQTLAIGPGMTDVTYFLSAGLSP
                     TLRRAHERDLVAAYHDELRRLGVTDYSPDQCWTDYRRMAVHGVFMGVFSAIAVERTER
                     GDALFLTMTRGACAQALDHDTFDLWSH"
     gene            1929..3164
                     /locus_tag="PLO65_09580"
     CDS             1929..3164
                     /locus_tag="PLO65_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD28539.1"
                     /translation="MTDKPLPSIVELTPLNPAYQSDPHAVLDDLRARCPAHRDAVSGS
                     LLLTRYADVRAVVNNRDLWRDPIRAEEGAIMQRRFLERAPEGVPRTHVTSILTLDDPD
                     HARIRQPLAQALYARVAKFRPQVEALVDETLDRLEGEREFDLMDRFCVPIPIDAIARI
                     LGVDPGRLAEFRQWSEGVIQGLNPFRTPEQTAIMEQASAALTDYFTQLMEDRRRSPRD
                     DLISDMVGQQAAGAALDDAELRLNLQALLVGGNLTTTDLIGNTVRNLLLNPSELAKLR
                     ADPGIVNAVVEEALRYEPPVDMTGRLASEDMEAGGCPLHPGQSITVSLRAANRDPDVF
                     EDPHRFDVSRKHRPHVAFGGGAHICIGAPLARLETGVALVRLFERFPTLRLADPGAEP
                     SWRALPFFRGMEHLPVLVD"
     gene            3238..3314
                     /locus_tag="PLO65_09585"
     tRNA            3238..3314
                     /locus_tag="PLO65_09585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3272..3274,aa:Pro,seq:tgg)
     gene            3345..3650
                     /locus_tag="PLO65_09590"
     CDS             3345..3650
                     /locus_tag="PLO65_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HRD28540.1"
                     /translation="MLARIYRPAKTAMQSGKAKTRDWLLEFEPAAAKRTDPLTGWTQS
                     TDMNGQVRLTFDTREEAVAFAQKHGIAFQLFEPKEPKKILKAYADNFATNRRQSWTH"
     gene            3808..3884
                     /locus_tag="PLO65_09595"
     tRNA            3808..3884
                     /locus_tag="PLO65_09595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3842..3844,aa:Arg,seq:tct)
     gene            complement(3887..>4466)
                     /locus_tag="PLO65_09600"
     CDS             complement(3887..>4466)
                     /locus_tag="PLO65_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRD28541.1"
                     /translation="DLSVQALTKYVCGHSDVFMGSVATTDAGIGARIAASVQDHGWAV
                     SPDDAYQALRGLRTLATRLDRHGESGVAVARWLANQPEVLSVLHPALPDDPGHRLWTR
                     DFSGACGLFGFVLRPAPPERVEAFLDALKLFGLGFSWGGFESLAINCDPQLKVRTLPV
                     ALGGPLLRLHVGLEAVDDLTADLRTGLDALAA"
     assembly_gap    4467..4573
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4574..4996)
                     /locus_tag="PLO65_09605"
     CDS             complement(<4574..4996)
                     /locus_tag="PLO65_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRD28542.1"
                     /translation="MTVGPPVQRGSTVLLPGARALYDGTLGYGRAGLAAQFALMDALA
                     ELEGAAAVQLFPSGLAALTAGIMAVLEAGDHLLVVDSVYKPVRQFCDKVLRRFGVTVD
                     YYDPRLDAAGLLSLAGPKTRLVVLESPGSLTFEMQDVEA"
BASE COUNT          778 a         1721 c         1718 g          680 t
ORIGIN      
        1 tggatcgccg cgcccatcac cgccgacagg cccaccgcgc cgccgccggt gatcagcaga
       61 ctcgagcccg tcgggcaggc gagggccagc atcacgccgc cggcgccggt ctggacgccg
      121 cagcccagcg gcgccgccag ggggaacggc aggtcgtcag gcagtttcag gacgttgcgc
      181 tcataggcga gggcgtaggt cgcgaacgac gactggccga agaagttgga cgagacggcc
      241 gtctcgccct ggcggatcgc cttgctgcca tccgggcgca tgccgatgta gttcagcgcc
      301 ggcatggagt agcagtacgc cggatcgccg ctccggcagc gatcgcactg gccgcaggag
      361 cgaaaggtca tggcgacccg atcgcccggc gccaccttgg tgaccgccgc cccgacccgc
      421 tcgaccacgc ccgcgccctc gtgtccgagt acggccggca gggagaaggg catcgcgcct
      481 tcacgggcca ccaggtcggt gtggcagacg ccgacggcgg cgacgcgaac gaggatctcg
      541 tcggcccgcg gctcgtcgag ctcgacggct tcgagggtga agtcggcgcc cggagcccgg
      601 gccacggcgg cggtcgcctg catggaagtg cctttcagtg tgaccagagg tcgaaggtgt
      661 cgtggtcgag cgcctgggcg caggcgccgc gggtcatggt caggaacagg gcgtcgcccc
      721 gctcggtgcg ctccacggcg atggccgaaa agacccccat gaacacgccg tgcacggcca
      781 tccggcgata gtcggtccag cactgatccg ggctatagtc cgtcacgccc agccggcgca
      841 gctcgtcatg ataggcggcc accaggtcgc gctcgtgggc gcggcgcagc gtcggcgaca
      901 ggccggcgct gaggaagtag gtgacgtcgg tcatgccggg gccgatcgcc agggtctgcc
      961 agtcgagcgt gcccatgcgg cgggttccgc cctggatgtc gaacaggatg ttgtcgagcc
     1021 ggaagtcgcc atgctggatg gtgaacggtc cctccgccgc cccctggaac aggcccatga
     1081 tctgcggaag ccggtcgacc agcgccatgt agtcggcctc cagcacgccg tcgtagcgtt
     1141 cgcggtaggc ggcgatgacg ccgggcaggg cggcgatgat cgccggatac atgtcggcgc
     1201 ccgccgcaag ccattcgatg tccctgagcg cggggtcgtt ccaccgcgga gcgtgcagcg
     1261 ccgcggcctc gagcatcgcg gtccgggcgt cggcgaccga acagccggtc agctggtcgc
     1321 cggcccgcgc cgggccgaaa tcctcgaaga tcaacgtgaa ggcggcggtc tgcgggtcga
     1381 tgccggcgaa atgggtcttc ggcgtgatga tgccgacggt cgaggccagt tcgcggtaga
     1441 agccgacctc gcggccatag aggttgaaag ccgctccgga ggcgcggctg gcggggtcgg
     1501 cggcggcgaa cttgcccacc acgctggcgg gggcggaggg cggcgcgccg tcataggtca
     1561 gggccaggcg gaagctgtcg ccgaccaggc cgttgccgac gggcgtaacg gtcaggccgg
     1621 tcacccggcc ccgtggcagg gcgcccgaag cccgcaacgc cgccgtcatc cagtcggggt
     1681 cgatctcgtc gacggtggcg gcgatgctcg gctcggtcat gtcgcattcc ccggccgccg
     1741 gcggatgttg gctccgcctg gcgacggctg gtccctgtgc aaggcctaga acatccgttc
     1801 gcggacggga accctgacgc cacggaaccg gggcgggatc agggattgcc gcagggtcag
     1861 gctttacggc ccggcatcct tggccgcatc ctgcccgcca ggctttccgc ctgtcccgga
     1921 ccctcgtgat gactgacaag ccgctgccct caatcgtcga gctgacgccg ctgaatccgg
     1981 cctaccagag cgacccccat gcggtgctcg atgacctgcg cgcccgctgc ccggcccatc
     2041 gcgacgcggt gtcgggcagt ctgttgctga cccgctacgc cgacgtgcgg gcggtggtga
     2101 acaaccggga cctctggcgt gacccgatcc gggccgagga gggcgccatc atgcagcggc
     2161 ggttcctcga acgggcgccc gagggcgtgc cccgcaccca tgtcaccagc atcctgacgc
     2221 tcgatgaccc cgaccacgcc cgcatccgcc agccgctggc ccaggctctc tacgcccggg
     2281 tggccaagtt ccgtccgcag gtcgaggcgc tggtcgacga gaccctcgac cggctggaag
     2341 gcgagcgcga attcgacctg atggaccggt tctgcgtgcc gatcccgatc gacgcgatcg
     2401 cccgcattct cggcgtcgac cccggccggc tggccgagtt tcggcaatgg tccgaaggcg
     2461 tgatccaggg tctcaaccct ttccgcacgc ccgagcagac agcgatcatg gagcaggcct
     2521 cggccgccct gaccgattac ttcacgcagc ttatggagga ccgccgccgg tcgccgcgcg
     2581 acgacctgat cagcgacatg gtcggccagc aggccgccgg cgcggcgctt gatgacgcag
     2641 agctgcgact gaacctgcag gcgctgctgg tcggggggaa cctgacgacc accgacctga
     2701 tcggcaacac cgtgcgcaac ctgctgctca acccctcgga actggccaag ctgcgggccg
     2761 accccggcat cgtcaacgcc gtggtcgagg aggccctgcg ctacgagccg ccggtcgaca
     2821 tgaccggtcg cctggcgtcg gaggacatgg aggcgggcgg ctgcccgctg catccggggc
     2881 agtcgatcac cgtctccctg cgcgcggcaa accgcgatcc ggacgtgttc gaggatccgc
     2941 accgcttcga cgtcagccgc aagcaccggc cgcatgtggc cttcggtggc ggcgcccata
     3001 tctgcatcgg ggcgccgctg gcccggcttg agacgggcgt ggccctggtc aggctgttcg
     3061 agcggtttcc gaccctgcgg ctcgccgatc cgggggccga gccgtcctgg cgggcgctgc
     3121 cgttcttccg cggcatggag cacctgccgg tgctcgtcga ctgagccggc cgcgcgtcgc
     3181 gcgctatccg tttgctccgg ccgtcgaacc tttgtagatc aagcccttcc gtagagacgg
     3241 ggtgtggcgc agcctggtag cgcatctgct ttgggagcag agggtcggag gttcgaatcc
     3301 tctcgccccg accacggaac gagatcgaac gaacgaggcc cgacatgctc gcccgcatct
     3361 accgcccggc caagaccgcc atgcagtccg gcaaggcgaa gacccgtgac tggctgctcg
     3421 agttcgagcc cgccgcggcc aagcgcaccg atcccctgac cggctggacc cagtcgaccg
     3481 acatgaacgg ccaggtgcgg ctgaccttcg acacccgcga ggaggccgtg gccttcgccc
     3541 agaagcacgg catcgccttc cagctgttcg agcccaagga gccgaagaag atcctcaagg
     3601 cctacgcgga caacttcgcg accaaccgac gccagtcctg gacacactga ccgcgctatc
     3661 gtgcgtcctt cgacacggcc cttcaggcct gcccgggacg acgtggaggg gtggccttca
     3721 gattccacgt cgtggaaaca gcgcgaagcg cgtgtccagc cacgctggct gttcccgccc
     3781 tccgaacatc gctaaaccac gcaacgagac cccatagctc aaccggatag agcacctgcc
     3841 ttctaagcag ggggttgcag gttcgagtcc tgctggggtc gccaggtcag gcggcgaggg
     3901 cgtccaagcc ggtgcgcagg tcggcggtca ggtcatcgac ggcttccagc ccgacgtgca
     3961 ggcggagcaa cgggccgccg agggccacgg gaagggtgcg gaccttcagc tgcgggtcgc
     4021 agttgatcgc caggctctcg aaaccgcccc aggaaaagcc caggccgaac agcttcaacg
     4081 catcgaggaa ggcctcgact cgttccggcg gggccggccg cagcacgaag ccgaacaggc
     4141 cgcaggcgcc gctgaagtcg cgcgtccaga gtcggtggcc cggatcgtcg ggcaaggccg
     4201 ggtgcagcac cgacagcacc tccggctggt tcgccagcca gcgggccacg gccacgccgc
     4261 tctcgccatg gcggtcgagc cgcgtcgcca gggtgcgcag gccgcgcagg gcctggtagg
     4321 cgtcgtcggg cgacaccgcc cagccgtgat cctgcaccga ggccgcaatc cgcgcgccga
     4381 tccctgcgtc cgtcgtggcc accgacccca tgaacacgtc ggaatggccg cagacatact
     4441 ttgtcagggc ctggacgctg aggtcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnggcctcc acgtcctgca tctcgaaggt cagggagccc ggagattcca
     4621 gcacgacaag ccgcgtcttc ggcccggcga gggacagcag gccggccgcg tccaggcgcg
     4681 ggtcatagta gtccacggtc acgccgaagc ggcgcagcac cttgtcgcag aactggcgca
     4741 ccggcttgta gacactgtcg acgacgagca gatgatcgcc ggcctcgagc acggccatga
     4801 taccggcggt cagggccgcg aggcccgagg gaaacagctg cacggccgcc gccccctcga
     4861 gctccgccag ggcgtccatc agggcgaact gcgcggccag gcccgcccgt ccgtagccga
     4921 gcgtgccatc gtacagggcc cgggcgccgg gcagcagcac ggtcgagccg cgctggaccg
     4981 gcggtccgac ggtcagggcc ggca
//
LOCUS       DAORNX010000200         4993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12510_length_4993_cov_7.430539, whole genome shotgun sequence.
ACCESSION   DAORNX010000200 DAORNX010000000
VERSION     DAORNX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12510_length_4993_cov_7.430539"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..998)
                     /locus_tag="PLO65_09610"
     CDS             complement(198..998)
                     /locus_tag="PLO65_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HRD28543.1"
                     /translation="MRTGFLVILGLLAACLARPAAAASAPNSPFNNWAAIVVAGDWRA
                     HDGGPTEAFDNARRDVAATLVGLGFRPETMTQFSTRPERYGDTRPERLTAQSMLDALK
                     ASAAAAPAGCLVYYTSHGAPQGTVLDVMGKMYMFSPAEMAALVDEACPNRPTIVIISA
                     CYSGVFVPYLKGAQRMVMTAARPDRSSFGCSSEDKYPFFDACFLESVGAVRNFASLPP
                     AIQGCVSRREGQMRLTPPSEPQVTIDAGLRLRLGMMPFPSGAQGVQSP"
     gene            complement(1003..1470)
                     /locus_tag="PLO65_09615"
     CDS             complement(1003..1470)
                     /locus_tag="PLO65_09615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD28544.1"
                     /translation="MTDVAAEQPQVLTDEAILARFLNAKRPPPGSQLLGFRMLRVSQA
                     DREVEVAFMATEQLTNPMKQIQGGYLCAMLDECMSVACMVASGMTSVAPTVEMKTSFF
                     RPALPGPITGVGRVVRWGKTIAFTEGELRDAEGRTLARATGTAMPTPFTRYKG"
     gene            complement(1478..1969)
                     /locus_tag="PLO65_09620"
     CDS             complement(1478..1969)
                     /locus_tag="PLO65_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HRD28545.1"
                     /translation="MAQQQKPPEDLMNYEALAQDALRGVVKAALKVAAAPGGIPGDHH
                     FYITFRTRAPGVSGPPDLLGKYPDEMTIVLQHQYWDLAPGETFFTVTLKFGGSPKKLS
                     VPYAAVTRFFDPHVQFGLQFEPPPLDVDAVTLDEPAPAPAPAAPPAAEGPKVVSLDQF
                     RKK"
     gene            complement(2075..2438)
                     /gene="ssrA"
                     /locus_tag="PLO65_09625"
     tmRNA           complement(2075..2438)
                     /gene="ssrA"
                     /locus_tag="PLO65_09625"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            2524..3186
                     /locus_tag="PLO65_09630"
     CDS             2524..3186
                     /locus_tag="PLO65_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRD28546.1"
                     /translation="MNKFIAEFIGTLVLVLFGCGAAVLGGDHVGQLGIALAFGFAIVA
                     MAYGIGPISGCHVNPAVSLAAFVAGRMSVRDMGVYWVAQFLGALAGAAILMVLAREGA
                     ASGLGRNGWGPDYLGEYSMQAALIFEIVFTAIFVIVILGVTGDKGHGAFAGLAIGITL
                     AVIHIVGIQVTGVSVNPARSFGPAVLVGGEALSQLWLFVVAPAIGAAIGGALFRFNLL
                     KA"
     gene            complement(3167..3817)
                     /locus_tag="PLO65_09635"
     CDS             complement(3167..3817)
                     /locus_tag="PLO65_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28547.1"
                     /translation="MDPLLYRLWSLPPLQTQLVRRGDLVLEIWARPTGGVRQAVIRVT
                     VRGDGKAFVQGRAGLGCCTPRIGRLVAFDEPLPADRIAAFRTLVDHPMWSQPRLTEVE
                     EPDGSASPLCIDGVAWDLTLVTARGSRNLHRACLDEEVGSVADALAPAVAAALGQDPR
                     FDLVFPRGAAFSAERAAYANLLERGGRLKPARADRPQPPMAPVPADPEPVATPSAD"
     gene            complement(4023..4574)
                     /gene="ilvN"
                     /locus_tag="PLO65_09640"
     CDS             complement(4023..4574)
                     /gene="ilvN"
                     /locus_tag="PLO65_09640"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRD28548.1"
                     /translation="MTTDPQPASAYDLVHADEVENLATFALLVDNEAGVLHRVVGLFA
                     ARGYNIESLTVAETDRKAHTSRITIVTRGAPHVLDQIEAQLSKVISVRRVHDVTRDPR
                     GIERELALVKVRGTGTDRVEALRVSDIFRAKVVDTTSESFVFEITGAPSKVDSFVELM
                     RPLGLVELSRTGVLSIERGAEGM"
     gene            complement(4583..>4993)
                     /locus_tag="PLO65_09645"
     CDS             complement(4583..>4993)
                     /locus_tag="PLO65_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD28549.1"
                     /translation="ASIQMCIQELSTAIQYDLPVKIFILNNEWMGMVRQWQQLLHGER
                     YSHSYSESLPDFVKLAEAYGAVGIRCSDPAELDAKILEMIDSPRPVVFDCRVEKGENC
                     LPMIPSGKAHNEMILPDDGADIGTVIDDAGRRLV"
BASE COUNT          762 a         1614 c         1818 g          799 t
ORIGIN      
        1 ccgccactgg cgcaggcgcg ggcgcctggg ccgtgctggc ggccggcagc ccgccgacga
       61 gaagggataa cgcagccgcg agtgcggctc tggctggcga tcgcaaggca tttccccggg
      121 gacagtcgtt cacggtgttc tagggcgccg gaagcggcgg gcaaaggtcg gtctgcgccc
      181 gccggtcgtg gagtccgtca gggtgactgg acgccctggg cgccgctggg aaatggcatc
      241 atgcccaggc gcagtcgcag gccggcgtcg atcgtcacct gcggctcgga cggcggggtc
      301 agccgcatct gcccctcgcg ccgggacacg cagccctgga tcgccggggg cagggacgcg
      361 aaattgcgca ccgcgccgac ggattcgagg aagcaggcgt cgaagaacgg atacttgtct
      421 tccgagctgc agccgaacga actgcggtcg gggcgggcgg cggtcatgac catgcgttgg
      481 gcgcccttca ggtagggcac gaagacgccc gagtagcagg cggagatgat cacgatcgtc
      541 ggccggttcg ggcaggcctc gtcgaccagg gcggccatct cggcgggcga gaacatgtac
      601 atcttgccca tcacgtccag caccgttccc tgcggcgcgc cgtgcgaggt gtagtagacg
      661 aggcatccgg ccggcgcggc cgccgccgag gccttgagcg cgtccagcat cgattgggcg
      721 gtcagccgtt cgggccgggt gtcgccatag cgttccggcc gggtcgagaa ctgggtcatg
      781 gtctcgggcc ggaaaccgag gccgaccagg gtcgcggcga cgtcgcggcg ggcgttgtcg
      841 aaggcctccg tcggaccgcc gtcatgggcg cgccagtcgc cggccacgac gatagccgcc
      901 cagttgttga acggactgtt gggcgcgctg gcggcggcag cgggacgagc gaggcaggcg
      961 gccagcaatc cgaggatcac gaggaagcct gtccgcatgt ccctagccct tgtaccgggt
     1021 gaagggcgtc ggcatcgccg tgccggtggc cctggccagg gtgcgcccct cggcgtcgcg
     1081 caactcgccc tctgtgaagg cgatggtctt gccccagcgg accacccggc ccacgccggt
     1141 gatcggcccg ggcagggcgg ggcgaaagaa gctggtcttc atctccaccg tcggcgcaac
     1201 gctggtcatg cccgaggcga ccatgcaggc cacgctcatg cattcatcga gcatggcgca
     1261 gaggtagccg ccctggatct gcttcatcgg gttggtcagc tgttcggtgg ccatgaaggc
     1321 gacctcgacc tcccggtcgg cctggctcac ccgcagcatg cgaaagccca gcagctgcga
     1381 gcccggcgga ggccgctttg cgttgagaaa ccgggccaga atggcctcgt cggtgagaac
     1441 ctgcggctgc tccgcggcga cgtcggtcac ctggccctca cttcttgcgg aactggtcga
     1501 gcgagacgac cttgggcccc tcggccgcgg gcggtgcggc cggcgccggg gccggcgcgg
     1561 gctcgtccag ggtgacggcg tcgacgtcga ggggcggcgg ctcgaactgc aggccgaact
     1621 gaacatgcgg gtcgaagaac cgcgtcacgg cggcgtaggg cacggagagc ttcttgggcg
     1681 agccgccgaa cttcagcgtc acggtgaaga aggtctcgcc cggcgccagg tcccagtact
     1741 ggtgttgcag gacgatggtc atttcgtcgg gatacttgcc cagcagatcg ggcggacccg
     1801 acacgcccgg ggcgcgggtg cggaaggtga tgtagaagtg gtggtcgccg gggatgccgc
     1861 cgggcgccgc cgccaccttg agggcggcct tgacgacgcc gcgcagggcg tcctgggcca
     1921 gcgcctcata gttcatcagg tcttcgggcg gtttctgctg ctgggccatg actcaaacgc
     1981 ctgcgtggac gcaaagccta gcctgtcgag gcggacccga aaagcggcgc ccccggcaaa
     2041 tggcggaatt tccggattcc catgtggaag gaaaggtggc ggggttctgt tggcaggccc
     2101 ccgccgggcc ccgcctgagg atctgaaccc ccaggagttt aggtcgagtg cgctagcacc
     2161 gcattacgcg gcgatcgcat actcttcagc gaagttatcg ttcgcagtta gtttagcggc
     2221 ccgaaacggt gagccacgcc gggacaaagc aacgccttta gacgtctgtc gatgctgatc
     2281 ggccccctgc ttccggcgat aacccgaaga aggaaatggt ggagccgccg ggaatcgcac
     2341 ccgggtccag tccgcttatt acgcgcgcgt ttatgtccat agtccgggca agcccgaaca
     2401 ggtccaatat agggacgaat ggttgcgggg ggaaggaggc cgcgacaggg acttcccgga
     2461 ctcagccaat tgggccagcc tcgccgaaca ccgctcgatg aagcggggga cgggagaagc
     2521 tccatgaaca agttcattgc cgagttcatc ggcacgcttg tgttggtatt gttcggctgc
     2581 ggtgcggcgg tattgggtgg agatcacgtc gggcagctgg gcatcgccct ggcgttcggc
     2641 ttcgccatcg tggccatggc ctacggcatc ggcccgatct cgggctgtca cgtcaatccg
     2701 gcggtcagcc tggccgcctt tgtcgccggc cggatgtcgg tcagggacat gggtgtgtac
     2761 tgggtggccc agtttctcgg cgctctggcc ggcgcggcca tcctgatggt tctggccagg
     2821 gaaggcgcgg ccagcggtct gggccgcaac ggctgggggc cggactacct gggtgagtat
     2881 tccatgcagg cggcgctgat cttcgagatc gtgttcaccg ccatcttcgt catcgtcatt
     2941 ctgggtgtca ccggcgacaa gggacatggc gcgttcgcgg gcctggccat cggtatcacc
     3001 ctggcggtga tccacatcgt cggcatccag gtcaccggcg tatcggtcaa tccggcgcgg
     3061 agcttcggcc cggccgtgct ggtcggcggc gaggcgttgt cgcagctgtg gctgttcgtg
     3121 gtcgccccgg ccatcggggc ggcgatcggc ggggcgctgt tccggttcaa tctgctgaag
     3181 gcgtagcgac cggctccgga tccgcgggga ccggggccat gggcggctgg gggcggtccg
     3241 cacgggcagg cttcagtcgt ccgccccgct ccaggaggtt cgcgtaggcg gcccgttcag
     3301 cgctgaacgc cgcgcctcgg ggaaacacca gatcgaaccg cgggtcctga ccgagggcgg
     3361 cggcgacggc tggcgcgagc gcgtcggcca ccgaaccgac ctcttcatcg aggcaggcgc
     3421 gatgcagatt ccggctgccc ctggcggtga ccagggtcag gtcccaggcg acgccgtcga
     3481 tgcacagggg cgacgccgag ccgtcaggct cctccacctc ggtcagccgc ggctgcgacc
     3541 acatcgggtg atcgaccaac gtccggaagg ccgcgatacg gtcggccggc agcggctcgt
     3601 caaacgccac cagccggccg atgcgcggcg tgcaacagcc caggccggcc cggccctgga
     3661 caaaggcctt gccgtcgcct cggacggtca cccggatcac ggcctggcga acgcctcccg
     3721 tgggccgggc ccagatttcc agcaccaggt cgccgcgccg gaccagctgt gtctgcagcg
     3781 ggggcaggct ccacaggcgg tagaggaggg gatccacccc gttgtcgatc ggcggcggac
     3841 gctcgccccg gcctgcccgg cgacctccca gcggatcggc ggcctcgtcc gggaccggcc
     3901 agggctccgt ccaccagagc gcgggatccg gcggcggaag ggtttccgga gcgaccggcg
     3961 gcggggcgcc ggcgagccag agcagggcga tcagcagggc gggcggcagg ttcgggtctc
     4021 ccctacatgc cctcggcgcc tcgctcgatc gagaggacgc cggtacggga cagttccaca
     4081 agtccgagcg gccgcatcag ttccacgaag ctgtcgacct ttgacggcgc gccggtgatc
     4141 tcgaacacga aactctcgga cgtggtgtcg accaccttgg cgcggaagat gtccgacacg
     4201 cgcaaggcct cgacgcggtc agtcccggtc ccgcgcacct tgaccagggc cagttcccgc
     4261 tcgatgccgc gcgggtcgcg ggtgacgtca tgcacccggc gcacgctgat caccttcgac
     4321 agctgcgcct cgatctggtc gaggacatgc ggcgcgccgc gggtgacgat ggtgatccgg
     4381 ctggtgtgcg ccttgcggtc ggtttcggcg acggtcaggc tctcgatatt gtagccgcgc
     4441 gcagcgaaca gcccgacgac gcggtgcagc actccggcct cgttgtcgac cagcagggcg
     4501 aaggtggcga ggttctcgac ctcgtcggcg tggacaaggt cgtaggccga ggcgggctgg
     4561 ggatcggtgg tcatggtccc tcctacacca gccgccggcc ggcgtcgtcg atgaccgtgc
     4621 cgatgtcggc gccgtcgtcg ggcaggatca tctcgttgtg ggccttgccc gacgggatca
     4681 tcggcaggca gttctcgccc ttctcgacgc ggcagtcgaa gaccaccggg cgggggctgt
     4741 cgatcatctc gaggatcttc gcgtccagct cggccggatc gctgcagcgg atgccgacgg
     4801 cgccataggc ctcggccagt ttgacgaagt cgggcaggct ttcggaatag ctgtgcgaat
     4861 agcgctcgcc gtgcagcagc tgctgccact ggcggaccat ccccatccat tcgttgttga
     4921 ggatgaagat cttgaccggc aggtcgtact ggatcgccgt cgacagctcc tggatgcaca
     4981 tctggatcga ggc
//
LOCUS       DAORNX010000201         4983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12541_length_4983_cov_7.154830, whole genome shotgun sequence.
ACCESSION   DAORNX010000201 DAORNX010000000
VERSION     DAORNX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12541_length_4983_cov_7.154830"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1582
                     /locus_tag="PLO65_09650"
     CDS             221..1582
                     /locus_tag="PLO65_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD28550.1"
                     /translation="MGSETPPGRIRARTIAAFSMATIPVGALTTPLLVHLPPHYAGLL
                     GLPLAAVGGIFFFVKVLAIFFDPAVGMFMDRTRTRIGRYRFWLVVAAPVLMLGVWMLF
                     SAQAGVGKAFLFGWLVVLYIGYSLIVLAQSAWGAVLATSYNERSRVYAWAHVVSTVGA
                     VSVLLLPSLLHLPEDGVVPMMGLMIVLSIPVTTLIVALGVPEPMVPRRAASDRLSLRD
                     FPALALRSTMRRLLLADLLFTLGPAITSPLYLFFMRQARGYSLVEANLMLLLFILAAI
                     IASPLWALAAYRFGKHRTLMAAAAAYSLAQAAIYFIPGKTFWLMAPSMFLAGGILAAL
                     SFLIRAMVADVGDEVRLETGKDRVGLLYALVSSTAKVGTASAVLITFAILDWVGFNAA
                     PGAANTPEAMRGLVLTFAIAPVILVMGGAFAVRGYSLSRERHAAIRSELDAKDAVLAE
                     SST"
     gene            complement(1579..>2638)
                     /locus_tag="PLO65_09655"
     CDS             complement(1579..>2638)
                     /locus_tag="PLO65_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRD28551.1"
                     /translation="RALDAIIDPRSGKGLAAAGLVQGLVLREGRAGFMLEVPAADAAR
                     YAPIREAAERLLAGLPGIEKAQVVLTASAPQGVTRQRKGARVAGDPKAELRPRKEVKR
                     PDNVKRVIAVASGKGGVGKSTVAVNLACAFAGLGLKVGLLDADVYGPSAPRMMGVDGE
                     PLFIDGKLQPLQAHGVTVMSIGFLVDEGSPMIWRGPMASSAVRQMVNDVRWATPEAPL
                     DILVIDMPPGTGDIHLTLVENFAVDGVVIVSTPQEIALIDARRAVGMFQKTGAKILGV
                     IENMAFFADPSTGAPIPIFGTGGARAEAEQLGVPLLAEIPIEIALRQAGDEGAPLTAS
                     DGHSAAVEAFRAAARALL"
     assembly_gap    2639..2789
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2790..3922
                     /gene="hflK"
                     /locus_tag="PLO65_09660"
     CDS             <2790..3922
                     /gene="hflK"
                     /locus_tag="PLO65_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRD28552.1"
                     /translation="WNDNATPGPWGSPPPSGGKGEPERPERGGPPPRGPGRGPGGTPP
                     DLNDLLRRVEAFLGGLTGGGGGGGQVPLRRMAPLAGGGLAALYVLAGVIVVGPREEAV
                     VTTFGAWSRSYGPGIGYHLPLVERSQKLDVTTLRTLSVGGDTGGGDTLMLTGDENIVD
                     LSFSVTWRVSNPGRYLFNVSDPEATLSAVAESAMREVVGRSELDAIISRSRGQIQIEV
                     AALMQRILDSYGSGIIIDGVQISDANPPKEVVDAFRDVNNASQNAESLANQARGTAAQ
                     VRNQAIGYRAQVVREAAGEAARFNQVYAQYKLAPGVTRDRLYIETMERVLSKTNKVII
                     DSKGASAPLILPPDVFRPRVAAPTAGAVTTPALPQRVSGDGQ"
     gene            3919..4776
                     /locus_tag="PLO65_09665"
     CDS             3919..4776
                     /locus_tag="PLO65_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRD28553.1"
                     /translation="MNNPRLTLYGIVAAVALIVLSQTFYIVDQTHQALVVRLGEVVRT
                     VNASPDDGPGLKVKVPFLESRLLFDKRNQPIQVVRQEILTANQERLMVDAFVRYRIND
                     PVRFYTGLGDDRTAKDRLNSVVNASLRQVLGSVTSEDIISRRRAALTRQVRDNLANRT
                     TRSRLGLQVIDVRIRSADLPEANQEAVFDRMKTARQQEAARIRAEGAQRQREIVATAT
                     QEAETIRGEADAERAKIFASSFGQDPSFAAYYRSLQAYEASLGQGTTLVLSPDSAFFR
                     YFSKGPSGQ"
     gene            complement(4821..>4983)
                     /locus_tag="PLO65_09670"
     CDS             complement(4821..>4983)
                     /locus_tag="PLO65_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517555.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRD28554.1"
                     /translation="DGANDLAMIEAAGLGVAYRAKPVVAARADARVDHADLTALLYFQ
                     GYRAEDFAA"
BASE COUNT          675 a         1777 c         1621 g          759 t
ORIGIN      
        1 ccaaggccta tttcgccggc ggcgagcagg gccagaagtt cttccgcaag atggccggcg
       61 gctgaacctg ccgcacaata tcgaatgcgg tcgccggttc cggcggccgt cacaagggga
      121 caggcgtcga cgcgcctgtc ctctttttta tttgctcttc cccagcggca tgatccgatc
      181 ctctccgtga cgcccgccag aggcgcacgg gagagacacc ttgggttccg aaacaccgcc
      241 gggccgcatc cgcgcccgca cgatcgccgc cttttcgatg gcgaccattc ccgtcggagc
      301 cctgacgacg ccgctgctgg ttcacctgcc gccccactac gccggactgc tcggcctgcc
      361 gctggccgcg gttggcggga tcttcttctt cgtcaaggtg ctggcgatct tcttcgaccc
      421 tgcggtcggc atgttcatgg accggacccg cacgcggatc ggtcgctacc gcttctggct
      481 ggtcgtcgcc gccccggttc tgatgctcgg ggtatggatg ctgttcagcg cccaggccgg
      541 cgtgggcaag gccttcctgt tcggctggct ggtggtgctc tacatcggct attccctgat
      601 cgtgctcgcc cagtcggcct ggggcgcggt gctggcgacc agctacaacg agcgatcgcg
      661 ggtctacgcc tgggcccatg tggtcagcac cgtgggcgcg gtgtcggtgc tgctgctgcc
      721 gtccctgctg cacctgcccg aggacggggt ggtgccgatg atggggctga tgatcgtgct
      781 gtcgatcccg gtcaccaccc tgatcgtcgc cctcggcgtg cccgagccga tggtcccccg
      841 ccgcgccgcc agcgatcgcc tctccctgcg cgacttcccg gcgctcgccc tgcgttcgac
      901 catgcgccgg ctgctcctcg ccgacctgct gttcacgctc ggcccggcga tcacctcgcc
      961 gctgtatctg ttcttcatgc gccaggcgcg cggctacagc ctcgtcgagg ccaacctgat
     1021 gctgctgctg ttcatcctgg ccgcgatcat cgcttcgccg ctgtgggcgc tggcggccta
     1081 ccgcttcggc aagcaccgta ccctgatggc cgcggcggcc gcctattcgc tggcccaggc
     1141 ggcgatctac ttcatcccgg gcaagacctt ctggctgatg gcgccgagca tgttcctcgc
     1201 cggcggcatt ctcgccgcgc tgagcttcct gatccgggcg atggtcgccg acgtcgggga
     1261 cgaggtgcgg ctggagaccg gcaaggaccg cgtcggcctg ctctatgcgc tggtctccag
     1321 caccgccaag gtcggcaccg cctcggcggt gctgatcacc ttcgccatcc tcgactgggt
     1381 cgggttcaac gccgcccccg gagccgccaa cacgccggag gccatgcgcg gcctggtgct
     1441 gaccttcgcc atcgccccgg tgatcctggt catgggcggc gccttcgccg tgcgcggcta
     1501 cagtctgagc cgcgagcggc atgcggcgat tcgttccgaa ctcgacgcga aggacgccgt
     1561 gctggcggaa tcctcgacct agagcagcgc ccgcgccgcc gccctgaagg cctcgaccgc
     1621 ggcgctgtgg ccgtccgagg cggtcagtgg cgcgccttcg tcgccggcct ggcgcagggc
     1681 gatctcgatg gggatctcgg ccagcagggg gacgcccaac tgctccgcct cggcccgcgc
     1741 cccgcccgtg ccgaagatcg ggatcggcgc gccggtcgac gggtcggcga agaaggccat
     1801 gttctcgatc acgccgagga tcttcgcccc ggttttctgg aacatgccca cggcccggcg
     1861 ggcgtcgatc agggcgatct cctgcggcgt ggagacgatc accaccccgt cgacggcgaa
     1921 gttctcgacc agggtcaggt ggatgtcgcc ggtgcccggc ggcatgtcga tgaccaggat
     1981 gtccagcggc gcctcgggtg tggcccagcg cacgtcgttg accatctggc gcacggcgga
     2041 cgaggccatc ggtccccgcc agatcatcgg tgagccctcg tcgacgagga aaccgatcga
     2101 catcaccgtg acgccgtggg cctgcaacgg ctgcagcttg ccgtcgataa acagcggctc
     2161 gccgtcgacg cccatcatcc gcggcgccga gggaccgtag acgtcggcgt cgagcagccc
     2221 gaccttcagg cccagcccgg cgaaggcgca ggccaggttg accgccaccg tcgacttgcc
     2281 gaccccgccc ttgccgctgg cgacggcgat cacgcgtttg acgttatcgg gccgtttgac
     2341 ttccttgcgc ggccggagtt cggccttcgg atcgccggcg acccgcgccc ccttgcgctg
     2401 gcgcgtgacg ccctgggggg ccgaggcggt cagcaccacc tgggcctttt cgattcccgg
     2461 caggccggcc agcagcctct cggcggcctc gcggatcggg gcgtagcggg cggcgtccgc
     2521 ggccggaacc tcgagcatga agccggcccg gccctcgcgc aggaccaggc cctgtaccag
     2581 gccggcggcg gctagaccct tgccggagcg ggggtcgatg atggcgtcga gcgcgcgann
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctggaatgac aacgccaccc ccggcccctg
     2821 gggctcgccg cccccgtccg gaggcaaggg tgagcccgaa cgtcccgagc gtggcggtcc
     2881 gccgccgcgc ggacccggac gcggccccgg cgggacaccc cctgatctca acgatctgct
     2941 gcgccgggtc gaggccttcc tcggcggcct gaccggcgga ggcggcgggg gcgggcaggt
     3001 gcccctgcgc cggatggccc cgctggccgg cggcggtctg gcggccctct atgtgctggc
     3061 cggcgtcatc gtggtcggtc cgcgcgagga ggcggtggtc accaccttcg gcgcctggag
     3121 ccgcagctac ggtcccggca tcggctacca tctgccgctc gtcgaacgat cgcagaagct
     3181 ggacgtcacc accctgcgca ccctgagcgt cggcggcgac accggcggtg gcgacaccct
     3241 gatgctgacc ggcgacgaga acatcgtcga cctgagcttt tcggtgacct ggcgggtctc
     3301 caaccctggc cgctacctgt tcaacgtgag cgatccggag gcgaccctct cggcggtggc
     3361 tgaaagcgcc atgcgcgagg tcgtggggcg gagcgagctc gacgccatca tcagccgcag
     3421 tcgcgggcag atccagatcg aggtcgccgc cctgatgcag cgcatcctcg acagctacgg
     3481 ctccggcatc atcatcgacg gcgtccagat cagcgacgcc aatccgccga aggaggttgt
     3541 cgacgccttc cgcgatgtga acaacgccag ccagaacgcc gagtcgctgg ccaaccaggc
     3601 ccgcggcacc gccgcccagg tgcgcaacca ggcgatcggc taccgcgccc aggtggtgcg
     3661 tgaggcggcc ggtgaggcgg cccggttcaa ccaggtctac gcccaataca agctggcccc
     3721 cggcgtcacc cgcgaccggc tctacatcga gaccatggag cgggtgctgt cgaagaccaa
     3781 caaggtgatc atcgacagca agggcgccag cgccccgctc atcctgccgc cggatgtctt
     3841 ccggccccgc gtggcggcgc cgaccgccgg cgcggtgacg accccggccc tgccccagcg
     3901 cgtttcggga gacggccaat gaacaatccc cgcctcaccc tctacggcat cgtcgccgcc
     3961 gtcgcgctga tcgtgctgag ccagaccttc tacatcgtcg accagacgca ccaggccctg
     4021 gtcgtccggc tcggcgaggt ggtccgcacg gtcaacgcct cgcccgacga cggccccggc
     4081 ctgaaggtca aggtcccgtt cctcgagtcc cgcctgctgt tcgacaagcg caaccagccc
     4141 atccaggtgg tccggcagga aatcctgacc gccaaccagg aacggctgat ggtcgacgcc
     4201 ttcgtgcgct atcgcatcaa cgacccggtg cgcttctaca ccggcctggg cgacgaccgg
     4261 acggccaagg accggctgaa ctcggtggtc aacgcctcgc tccgccaggt gctgggctcg
     4321 gtcacgtcgg aggacatcat ctcgcgccgc cgcgccgccc tgacccgtca ggtgcgcgac
     4381 aacctcgcca accggaccac ccgctcgcgc ctgggcctgc aggtcatcga cgtgcgcatc
     4441 cgcagcgccg acctgccgga ggccaaccag gaagccgtat tcgaccggat gaagacggcc
     4501 cgccagcagg aggcggcccg catccgcgcc gagggcgccc agcgccagcg cgaaatcgtc
     4561 gccacggcca cgcaggaggc cgagacgatc cgcggcgagg ccgacgccga acgggcgaag
     4621 atcttcgcct ccagcttcgg ccaggacccg tccttcgcgg cctactatcg ttcgttgcag
     4681 gcctatgagg cgtcgctcgg ccagggcacg accctggtcc tgtcgcccga cagcgccttc
     4741 ttccgctact tcagcaaggg cccgtccggg cagtagcgtc agggggacat gtaccgcctt
     4801 cggtacgtgt cccttccggg ctacgccgcg aaatcctcgg cccggtagcc ctggaagtac
     4861 agcagggccg tcaggtcagc gtggtcgacg cgggcgtcgg cccgggcggc gacgaccggc
     4921 ttggcgcggt aggcgacgcc cagccccgcg gcctcgatca tcgccaggtc gttggcgccg
     4981 tcg
//
LOCUS       DAORNX010000202         4961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12615_length_4961_cov_7.595393, whole genome shotgun sequence.
ACCESSION   DAORNX010000202 DAORNX010000000
VERSION     DAORNX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12615_length_4961_cov_7.595393"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /gene="metG"
                     /locus_tag="PLO65_09675"
     CDS             complement(<1..1458)
                     /gene="metG"
                     /locus_tag="PLO65_09675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRD28555.1"
                     /translation="MARILITSALPYINGIKHLGNLAGSMLPADVFARFQRARGVETL
                     YICATDEHGTPAELAAAAAGQDVAAYCAEQHDLQKTIGARFGLSWDWFGRSSNAPNHR
                     LTQHFCEALENNGLIEERTDKMVYSIDDRRFLPDRYVEGTCPRCGFDKARGDQCDNCG
                     ELLDPVDLINPYSVISGSRNIEVRDTRHLYLLQTKIEGKIRAWVDSRAEGWPALTRGI
                     AYKHLDEGLIDRGITRDLAWGIPVVRDGKPRPGFENKVFYVWFDAPIEYIGATVEWSE
                     ATGADWRRWWRTDAGATDVDYVQFMGKDNVAFHTVSFPATIIGSGEPWKQVDRLKAFN
                     WLNWYGGKFSTSGRRGVFMDAALELLPADLWRWYLTANAPEGSDTAFTWEQFQSAANK
                     DLADVLGNFINRIVRFNESKFGPAVPAGGEPGPREQQLYADVAAQLAVLTAELEAIEV
                     RKAAQAVRALWVLGNEYLQEAAPWTAFKTDVDRAAV"
     gene            1596..2018
                     /locus_tag="PLO65_09680"
     CDS             1596..2018
                     /locus_tag="PLO65_09680"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRD28556.1"
                     /translation="MKGINWIGVIAAVVASQIIGTAWYGYLFAAQWTQLSGLSAESME
                     AAGGLTYAFGALQNLVVAVGLAWLGVKTGAVGWAGGAKTGLFACVFFALATYALRFIY
                     GDDNPGLIPIDGGYMLIQYLVSGALVCGLRIGKAPAAA"
     gene            2131..3240
                     /locus_tag="PLO65_09685"
     CDS             2131..3240
                     /locus_tag="PLO65_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD28557.1"
                     /translation="MTGQTRRWTAGVLGAAALALAACGGGGDKTPTLRIYNWSDYIDP
                     ALLDEFTKETGIKVVYDTFDSNEVLETKVLTGGTGYDIVAPSNHNVPRYVTAGALQAL
                     DFSKLPNRKNLWPDLMARMEPFDPGGKYTVPYMWGTMGIGYNTAEIAKRLPGVALDSW
                     DVVFKPENLAKLKDCGVYFLDASEDMYAVTLNYLGKDPNSRNVADYEAATNLLISLRP
                     YVTKIHSSEYINALANGDACIAIGYSGDILQAATRAEEAKNGVEISYVIPREGSQVWF
                     DVFAIPADAPNKDAAYKFLDYMLRPAVIARASNYTEYANANLPATPLVEESLRTNPNV
                     YPTPEVFGRLFVTTTKDQALLQQVNRLWTKVMTGR"
     gene            3237..4430
                     /locus_tag="PLO65_09690"
     CDS             3237..4430
                     /locus_tag="PLO65_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD28558.1"
                     /translation="MSRPPASKAPRGEPGQRRNIGAVRSSFAPWNDPNATPLVRFEGV
                     TKRFGDHAAVNDVSLDIYPGEFFALLGPSGCGKTTLMRMLAGFETLDAGRITLNGEDL
                     AGAPPHRRPVNMMFQSYALFPHLTVEQNIAFGLKQEGMPKKEIAARVAEMLALVKLEP
                     FARRRPDQLSGGQKQRVALARAVARKPRMLLLDEPLGALDKKLREETQFELMDLQVQL
                     GVTFLIVTHDQEEAMVMADRIAVMRDGRIVQIGRPEEVYEAPNSRYVAEFLGDVNLFE
                     TTVEAIEHPMAHLNLVESDAGFHVLDDDCPGPGSTVWLAVRPEKMRISLDPPAAGARN
                     VLAGEIWDIGYTGDWTNYIVELPGGKLVRVARANARRFVDRPIDWDDKVWVSFDPDAG
                     VVLTS"
     gene            4552..>4961
                     /locus_tag="PLO65_09695"
     CDS             4552..>4961
                     /locus_tag="PLO65_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HRD28559.1"
                     /translation="MSRPRSRAAILPAVWLGLFFAFPFLLVLRLSLSDTALAIPPYTP
                     QIVWANGLEGIRAFLAALDGEMYRRLVSERLYVDAYLSSLRFAATGTVLALLVGYPMA
                     YGMARSRPGVRSVLLMLVILPFWTSFLIRVYAWVG"
BASE COUNT          790 a         1694 c         1672 g          805 t
ORIGIN      
        1 caccgcggcg cggtcgacgt cggtcttgaa cgccgtccag ggggcggctt cctgcagata
       61 ttcgttgccc agcacccaca gggcgcggac agcctgggcc gccttgcgca cctcgatggc
      121 ctcgagctcg gcggtcagca cggccagctg ggcggcgacg tcggcgtaga gctgctgctc
      181 gcgcgggccc ggctcgccgc cggccgggac ggccgggccg aacttgctct cgttgaagcg
      241 cacgatgcgg ttgatgaagt tgcccagcac gtctgccagg tccttgttcg ccgccgactg
      301 gaactgttcc caggtgaagg ccgtgtctga cccttccggc gcattggccg tgaggtacca
      361 gcgccacagg tccgcaggca gcagctccag cgccgcgtcc atgaacacgc cgcgtcggcc
      421 gctggtggag aacttgccgc cgtaccagtt gagccagttg aacgccttga gccggtcgac
      481 ctgcttccac ggctcgcccg agccgatgat cgtcgccggg aaactgaccg tgtggaaggc
      541 cacattgtcc ttgcccatga actggacgta gtcgacatcg gtcgcgcccg cgtcggtgcg
      601 ccaccagcgc ctccagtcgg cgccggtcgc ctcggaccat tcgacggtgg cgccgatgta
      661 ttcgatcggc gcgtcgaacc agacgtagaa caccttgttc tcgaagcccg ggcgcggctt
      721 gccgtccctg accaccggga tgccccaggc caggtcacgg gtgatgccgc ggtcgatcag
      781 accctcgtcg aggtgcttgt aggcgatgcc acgcgtcagg gccggccagc cctcggccct
      841 ggaatcgacc caggcgcgga tctttccctc gatcttcgtc tgcagcaggt agaggtggcg
      901 cgtgtcgcgc acctcgatat tccgcgagcc gctgatcacc gaatagggat tgatgaggtc
      961 gacggggtcg agcaactcgc cgcagttgtc gcactggtcg ccgcgcgcct tgtcgaagcc
     1021 gcagcgcggg caggtcccct cgacgtagcg atccggcagg aagcgcctgt cgtcgatcga
     1081 gtagaccatc ttgtcggtcc gctcctcgat caggccgttg ttctccagcg cctcgcagaa
     1141 atgctgcgtc aggcggtgat tgggcgcatt cgagctgcgg ccgaaccagt cccagctcag
     1201 gccgaaacgg gcgccgatgg tcttctgaag gtcgtgctgt tcggcgcaat aggcggccac
     1261 gtcctgaccc gccgcggccg cggccagttc ggccggcgtg ccatgctcgt cggtggcgca
     1321 gatgtacagg gtctcgacgc cccgcgcccg ctggaagcgc gcgaacacgt ccgccggcag
     1381 catagagccc gccaggttgc ccaggtgctt gatcccgttg atgtacggca gggcggaggt
     1441 gatgaggatg cgggccatgg tcggcggcta tagccgcccc tcccccgctt ggaaagtttg
     1501 gcgactcgca gaacggcgtt accctggcgt caaccgggcg agcggggcgc ccggagagcg
     1561 gctcggcccc gcgacgacat ggggagaccg tcgtcatgaa aggcatcaac tggatcggcg
     1621 tgatcgccgc ggtcgtggcc agccagatca tcggcacggc ctggtacgga tacctgttcg
     1681 ccgcccaatg gacgcagctg agcggcctgt ccgccgaaag catggaggcc gccggcggcc
     1741 tgacctacgc ctttggcgcg ctgcagaacc tcgtcgtcgc cgtgggcctg gcctggctgg
     1801 gcgtgaagac cggcgcggtc ggctgggccg gcggcgcgaa gaccggcctg ttcgcctgcg
     1861 tgttcttcgc cctggccacc tacgcgctgc ggttcatcta tggcgacgac aatccgggcc
     1921 tgatcccgat cgacggcggc tacatgctga tccagtacct ggtcagcggc gcgctggtct
     1981 gcgggctgcg gatcggaaag gcgccggccg ccgcgtgagg cgatggccga tcgggcggga
     2041 cgcccgttcg gcacatgtcc ccttcctccc cggacgccca aggccctata aggatttcca
     2101 gccttgtggg gagacttggg gatgtccggt atgacgggtc agacgcggcg gtggaccgcc
     2161 ggggttctgg gcgccgcggc gctcgcgctg gcggcttgcg ggggcggcgg ggacaagacg
     2221 ccgaccctgc gcatctacaa ctggtcggat tacatcgacc cggcgctgct ggacgagttc
     2281 accaaggaga ccggaatcaa ggtcgtctac gacaccttcg actccaacga ggtgctcgag
     2341 acaaaggtgc tgaccggcgg caccggctat gacatcgtcg cgccgtcgaa ccacaacgtg
     2401 ccgcgctatg tcaccgccgg cgccctgcag gccctcgact tcagcaagct gccgaaccgc
     2461 aagaacctgt ggcccgacct gatggcccgg atggagccgt tcgatcccgg cggcaaatac
     2521 accgtgccct acatgtgggg caccatgggc atcggctaca acaccgcgga gatcgccaaa
     2581 cgcctgcccg gcgtggccct cgacagttgg gacgtggtgt tcaagccgga gaacctcgcg
     2641 aagctgaagg actgcggcgt ctacttcctc gacgcgtccg aggacatgta cgcggtcacg
     2701 ctgaactacc tgggcaagga tcccaattcg cgcaatgtcg ccgactacga ggccgcgacc
     2761 aacctgctga tcagcctgcg gccctacgtc accaagatcc attcgtcgga gtacatcaac
     2821 gccctggcca acggcgacgc ctgcatcgcc atcggctaca gcggcgacat cctgcaggcc
     2881 gccacccggg ccgaggaggc caagaacggc gtcgaaatca gctatgtcat tccccgggag
     2941 ggcagccagg tctggttcga cgtgttcgcc attccggccg acgcccccaa caaggacgcc
     3001 gcctacaagt tcctcgacta catgctgcgg ccggcggtga tcgcccgggc gtcgaactat
     3061 accgagtacg ccaacgccaa tctgccggcg acaccgctgg tcgaggagag cctgcgcacc
     3121 aaccccaacg tctatccgac gccggaggtg ttcggccggc tgttcgtcac caccaccaag
     3181 gaccaggccc tgctgcagca ggtcaaccgg ctctggacga aggtgatgac cgggcgatga
     3241 gccggccgcc ggcttcgaag gccccgcgcg gcgagccggg ccagcgccgc aacatcggcg
     3301 ccgtgcgctc gagcttcgcg ccgtggaacg accccaacgc cacgccgctc gtgcggttcg
     3361 agggggtgac caagcggttc ggcgaccacg ccgcggtgaa tgacgtctcg ctggacatct
     3421 atccgggaga gttcttcgcc ctgctcggac cgtccggctg cggcaagacc accctgatgc
     3481 ggatgctggc cgggttcgag acgctcgacg ccggccgcat caccctgaac ggcgaagacc
     3541 tggccggcgc gcccccgcac aggcggccgg tcaacatgat gttccagagc tacgcccttt
     3601 tcccgcacct gaccgtcgag cagaacatcg ccttcggcct caagcaggag gggatgccga
     3661 aaaaggagat cgccgcccgc gtcgccgaga tgctggccct ggtcaagctg gagccgttcg
     3721 ccaggcgccg cccggaccag ctgtcgggcg ggcagaagca gcgggtggcc ctcgcccgcg
     3781 ccgtggcccg caagccgcgc atgctgctgc tggacgagcc gctcggcgcg ctggacaaga
     3841 agctgcgcga ggaaacccag ttcgagctga tggacctgca ggtccagctg ggcgtgacct
     3901 tcctgatcgt gacccacgac caggaggagg ccatggtcat ggccgaccgc atcgcggtga
     3961 tgcgcgacgg caggattgtc cagatcgggc ggcccgagga ggtctacgag gcgcccaaca
     4021 gccgttatgt cgccgagttc ctcggcgatg tgaacctgtt cgagaccacc gtcgaagcca
     4081 tcgagcatcc gatggcgcac ctgaacctcg tcgagtccga cgcgggcttc catgtgctcg
     4141 acgacgactg ccccgggccc ggatcgaccg tctggctggc cgttcggccc gagaagatgc
     4201 gcatcagcct cgatcccccc gccgccggcg cccgcaacgt gctggccggt gagatctggg
     4261 acatcggcta caccggcgac tggaccaact acatcgtcga actgcccggc gggaagctgg
     4321 tgcgggtggc ccgggccaac gcgcggcgtt tcgtcgaccg gccgatcgac tgggacgaca
     4381 aggtgtgggt cagtttcgac cccgacgccg gcgtggtact gacctcgtga cgggcgtggc
     4441 atccagcatg ttgcgtggtt cgacacgcac cctgcgggtg ctgctcacca tgacgtgcac
     4501 agagcgacgt catcctgagc agcgcgaagc gcgtgtcgaa ggacgcagct cgtgagccgc
     4561 ccgaggtccc gcgcggccat cctcccggct gtctggctgg gcctgttctt cgccttcccg
     4621 ttcctgctgg tgctgcggct gtcgctgtcg gacacggccc tggccattcc gccctacacg
     4681 ccgcagatcg tctgggccaa cgggctggag ggcatccgcg ccttcctcgc cgcgctcgat
     4741 ggcgagatgt accgacggct ggtgtcggag cggctctatg tcgacgccta cctgtcgagc
     4801 ctgcgcttcg cggccacggg cacggtgctg gcgctgctgg tcgggtatcc gatggcctat
     4861 ggcatggcca ggagccggcc gggcgtgcgc agcgtgctgc tgatgctggt catcctgccg
     4921 ttctggacca gcttcctgat ccgggtctac gcctgggtcg g
//
LOCUS       DAORNX010000203         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12719_length_4933_cov_7.584461, whole genome shotgun sequence.
ACCESSION   DAORNX010000203 DAORNX010000000
VERSION     DAORNX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12719_length_4933_cov_7.584461"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLO65_09700"
     CDS             <1..925
                     /locus_tag="PLO65_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28560.1"
                     /translation="GAGTGTLPNASWTFRPLQGVKARSGVAGLEPRTLAAEEDERFAL
                     ALLDQGAIALERAEFAAEAADADALRRSDRLRSALLNSVSHDLRTPLSTVLGAVTTLI
                     EFGKSMQPKVRTDLLLSIREEAERLNRYVGDLLDMTRLEGGALNTRKDWTDVRDVLAA
                     AIGRVERRLEGRRLDRDFPAELSMVMADPSLLEQALVNILENAIAYSPDGSVIETAAY
                     EDRGNVVISIEDEGRGIPTAELERVFERFRRMEEATDRGKGAGLGLAIAKGFVEAMGG
                     RIAAASPIHDGRGTRILISLRKETVTPGHML"
     gene            922..1608
                     /locus_tag="PLO65_09705"
     CDS             922..1608
                     /locus_tag="PLO65_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28561.1"
                     /translation="MTAYRPRILVIDDEPQIHRFLGPALDASGYEHVRADDAASGLRE
                     IARKPPDAVVLDLGLPDMDGKEALARARAFYGGPILILSARDRETEKIDALDAGADDY
                     VEKPFGVGELLARLRVAMRHRLQRDGAEPVIRSGDVEIDLVNRRIVRAGEPVRLSPKE
                     YDLLACLAGGAGRVLTHRQILTAVWGPAHEEDVQYLRVFVGQLRQKLEADPAAPQIIL
                     TEPGVGYRFG"
     gene            complement(1625..2353)
                     /locus_tag="PLO65_09710"
     CDS             complement(1625..2353)
                     /locus_tag="PLO65_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRD28562.1"
                     /translation="MRPIGLQLYTLRKPFAADPLGTLSRIREIGYDAVEFAAPLDADF
                     AAFAARMKDIGLDCPSAHVGLADMAERPDAVLAMAGTLGCRYLVMPYVMPDQRDWGAV
                     IATLGAFARRARDEGLRVAYHHHDFEFETTDGVRPFDQLVGETDPDLVDFELDVYWLK
                     KGGEDPETFIEKMTGRVKLIHLKDMAPDGGMADVGAGTLDFAALLQAADSAGAQHLFV
                     EHDFPPAPWWPSVEASLGHLRTLD"
     gene            complement(2358..4049)
                     /locus_tag="PLO65_09715"
     CDS             complement(2358..4049)
                     /locus_tag="PLO65_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRD28563.1"
                     /translation="MFDAIVVGSGITGGWAAKELCERGLKVLMLERGPDVPHGPGYSH
                     DLAASLRRRDDQIPQDERDLHYPYHVGVSYALFNSNKAFWASDHDHPYETAPGKPYRW
                     IRGYQLGGRSLTWGRQSYRWAPQDFLSNLQDGHGVDWPIRYEDLEPWYDHVEAFAGIS
                     GDYDGLDQLPDGDFLPAFPFNDVELAAKARIEADYPTRRMIMGRTAHLSRVAKAQMDL
                     GRKRCEQHMRCYHGCPLGSYFSTQAATLPAALNTGNLTVLTDRVVESVIHDPATGRVT
                     GVRTVNRQDKTGETFEARIVFLNASAINTAALLLNSRSEAFPRGLANGSDQVGRNLMD
                     HVSTTSIVGKMPGFGDVYDQRDRPTGIYVPRYGNVTEADKPYLRGFGMQGGAARSRRS
                     EGAIQGFASGDEDDGLRGWHFSLLPFGEVLPDPDNRVTLSATRKDSWGIPVPIIEAAH
                     GPNEYAMMRAAARDASEMLRAAGCVDITPWEEAAEQLTPPGDRIHEMGTARMGRDPAT
                     SVLNAWNQTHEVANLFVTDGSVMTSSASVNPSLTYMAVTARAANHAADLLEQGGL"
     gene            complement(4049..4819)
                     /locus_tag="PLO65_09720"
     CDS             complement(4049..4819)
                     /locus_tag="PLO65_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRD28564.1"
                     /translation="MIRQSFAWWSFTLGREIEPETLLREAAAAGAQGVEMLPESLWPV
                     AQDVGLSIVTLTGHTLERGFNDRSLHAALADEVRRNIDTAAAGGCEAVIVFSGDRIGD
                     GADQPAIAACVEGLAPVVAHAEAAGVRLLLELLNSKVDHPGHQCDRTAFGAAVVRGVE
                     SPALRLLYDGYHMQLMEGDLMRTIRANIDLIGHVHTAGAPGRRDLDDRQEINWTAIAG
                     LLRHLDYAHWVGHEFVPRGEPVPALRAAISQFGLKGAA"
BASE COUNT          753 a         1724 c         1708 g          748 t
ORIGIN      
        1 cggcgccggc accggcaccc tgcccaacgc cagctggact ttccgcccgc tgcagggggt
       61 caaggcccgg tcgggcgtcg ccgggctcga accccggacc ctggccgccg aggaggacga
      121 acggttcgcc ctggccctgc tcgaccaggg cgccatcgcg ctggagcgcg ccgagttcgc
      181 cgccgaagcc gccgacgccg acgccctgcg ccggtcggac aggctgcgct cggccctgct
      241 caactcggtc agccatgacc tgcgcacgcc gctgtcgacg gtgctgggcg cggtcaccac
      301 cctgatcgag ttcggcaagt cgatgcagcc gaaggtccgc accgaccttc tgctttccat
      361 tcgcgaggag gccgagcggc tgaaccgcta cgtcggcgac ctgctcgaca tgacccggct
      421 cgagggcggc gcgctcaaca cccgcaagga ctggaccgat gtgcgcgacg tgttggccgc
      481 cgccatcggc cgggtcgagc gtcggctcga gggacgcagg ctggaccgcg attttcccgc
      541 cgaactgtcg atggtcatgg ccgacccgtc gcttctggaa caggcgctgg tcaacatcct
      601 cgagaacgcc atcgcctaca gtcctgacgg gtcggtcatc gagacggcgg cctacgagga
      661 ccggggcaat gtggtgatct cgatcgagga cgaagggcgg ggcattccga ccgccgaact
      721 ggagcgggtg ttcgagcgat tccggcggat ggaggaggcc accgaccgcg gcaagggcgc
      781 aggcctcggc ctggccatcg ccaagggctt cgtcgaggcc atgggcggtc gcatcgcggc
      841 ggccagcccg atccacgatg gacgcggcac ccgcatactg atcagcctgc gcaaggaaac
      901 cgtcaccccg ggccacatgc tttgaccgcc taccgccccc gaatcctcgt catcgacgac
      961 gagccgcaga ttcaccgctt cctcggtccg gcgctcgacg cctcgggcta tgagcatgtc
     1021 cgcgccgacg acgcggcgag cggcctgcgc gagatcgccc gcaagccgcc cgacgcggtg
     1081 gtgctcgacc tgggtctgcc cgacatggac ggcaaggagg ccctggccag ggcgcgcgcc
     1141 ttctacggcg gcccgattct gatcctgtcg gcgcgcgacc gcgagaccga aaagatcgac
     1201 gccctcgacg ccggcgccga tgactatgtc gaaaagccgt tcggcgttgg cgagctgctg
     1261 gcccgcctgc gcgtggccat gcgccaccgg ctgcagcggg acggcgccga accggtcatc
     1321 cgctcaggcg atgtcgagat cgacctggtc aaccgccgta tcgtccgggc cggcgagccg
     1381 gtccgcctct cgcccaagga gtacgacctg ctggcctgcc tggcgggcgg cgccggccgg
     1441 gtcctgaccc accgccagat cctgaccgcg gtctgggggc cggcgcacga ggaggacgtg
     1501 cagtatctgc gcgtgttcgt cggccagctg cgccagaagc tggaggcgga ccccgccgcc
     1561 ccgcagatca tcctgaccga gccgggtgtc ggctaccggt tcggctgaac ggacgccgac
     1621 gccgtcagtc cagtgtccgc agatgcccca ggctggcctc cacgctgggc caccaggggg
     1681 ccggcgggaa atcgtgttcg acaaacagat gctgggcgcc ggcggagtcc gcggcctgga
     1741 gcagggccgc gaaatcgagg gttccagcgc cgacgtcggc catccctccg tcgggggcca
     1801 tatccttgag atggatgagc ttcacccgcc cggtcatctt ctcgatgaag gtctccggat
     1861 cctcgccgcc ctttttcaac cagtagacgt cgagctcgaa gtcgacgagg tccgggtcgg
     1921 tctcgcccac cagctggtcg aacggccgga cgccgtcggt cgtctcgaac tcgaagtcgt
     1981 ggtggtggta ggcgacgcgc aggccctcgt cgcgggcgcg ccgggcgaag gcgccgaggg
     2041 tcgcgatgac cgcgccccag tcacgctggt cgggcatcac atagggcatg accaggtagc
     2101 gacagcccag ggtcccggcc atcgccagca cggcgtcggg ccgctcggcc atgtcggcaa
     2161 ggcccacatg ggcagaagga cagtcgagcc cgatgtcctt catccgcgcc gcgaaggccg
     2221 cgaaatcggc atcgagcggc gcggcgaact cgaccgcatc gtagccgatc tcgcgaatgc
     2281 gcgagagcgt gcccagcgga tccgcagcga acggcttgcg cagggtgtag agctgaaggc
     2341 cgatcggtcg catgggctta caagcctccc tgttcgagta gatccgccgc gtggttcgcc
     2401 gcccgggcgg tcacggccat ataggtcagc gacgggttca ccgaggcgct ggaggtcatc
     2461 accgagccgt ccgtgacgaa caggttggcg acctcatgcg tctggttcca ggcgttcagc
     2521 accgaggtcg ccggatcgcg gcccatgcgc gcggtgccca tctcgtggat gcggtcgcca
     2581 ggcggggtca gctgctcggc ggcctcctcc cagggggtaa tgtcgacaca gccggcggcc
     2641 cgcagcatct ccgacgcgtc ccgcgccgcc gcccgcatca tcgcgtattc attaggcccg
     2701 tgggccgcct cgatgatcgg caccgggatc ccccagctgt ccttgcgcgt ggccgacagg
     2761 gtgacgcggt tgtcgggatc gggcagcacc tcgccgaacg gcagcagcga gaagtgccaa
     2821 ccgcgcagcc cgtcatcctc atcgccggac gcgaaaccct ggatggcccc ttcgctgcgc
     2881 cgcgacctgg cggcgccgcc ctgcatgccg aagccgcgca ggtagggctt gtccgcctcg
     2941 gtgacattgc catagcgcgg gacgtagatg ccggtgggcc ggtcacgctg gtcgtagacg
     3001 tcgccgaacc ccggcatctt gccgacgatc gacgtggtgc tgacgtgatc catcaggttg
     3061 cggccgacct ggtcgctgcc gttggccagg ccgcgcggga aggcttcgga ccttgaattc
     3121 aacaacagcg cggcggtgtt gatcgccgag gcgttcagga agacgatccg cgcctcgaac
     3181 gtctcgccgg tcttgtcctg gcggttgacg gtcctgacgc cggtcacccg gcccgtcgcc
     3241 ggatcatgga tgacgctttc gacgacgcgg tcggtcagca ccgtcaggtt gcccgtgttg
     3301 agggcggcgg gcagggtcgc ggcctgggtc gagaaatagc tgcccagcgg acagccgtga
     3361 tagcagcgca tatgctgttc gcagcgcttt cgccccaggt ccatctgggc cttggcgacc
     3421 cgactgaggt gcgccgtccg ccccatgatc atccgccgcg tcgggtagtc ggcctcgatc
     3481 ctggcctttg cggccagctc cacgtcgttg aaggggaagg ctggcaggaa gtcgccgtcg
     3541 ggcagctggt cgaggccgtc gtagtctccg ctgatccctg cgaaggcttc gacatggtcg
     3601 taccagggct caaggtcctc gtagcggatc ggccagtcga cgccgtgtcc gtcctgcagg
     3661 ttggacagga agtcctgcgg cgcccagcgg taggattgcc gcccccaggt cagcgaccgc
     3721 ccgcccagct gataaccccg gatccagcga tagggcttgc cgggggccgt ttcgtagggg
     3781 tggtcgtgat cactggccca gaaggccttg ttggagttga acagggcgta ggagaccccg
     3841 acgtggtagg ggtagtgcag gtcccgctcg tcctgcggga tctggtcgtc ccgccggcgc
     3901 agggaggcgg ccaggtcgtg gctgtagccc ggcccgtgcg gcacgtcggg accgcgttcg
     3961 agcatcagga ccttgaggcc gcgctcgcag agctccttgg ccgcccagcc gccggtgatc
     4021 ccggagccga cgacgatggc gtcgaacatc acgccgctcc cttcaacccg aactgcgaga
     4081 tggccgcccg caacgccggg acaggctcgc cacggggcac gaattcatgt ccgacccaat
     4141 gcgcgtagtc cagatgccgc agcagcccgg cgatggccgt ccagttgatt tcctggcgat
     4201 cgtccagatc acggcgaccg ggcgccccgg cggtatggac gtggccgatc agatcgatgt
     4261 tggcgcggat cgtccgcatc agatcgccct ccatcagctg catgtgatag ccgtcgtaga
     4321 gcagccgcag ggcgggcgac tccacgcccc ggaccaccgc cgccccgaag gccgtgcgat
     4381 cgcactggtg gccgggatgg tcgaccttgc tgttgagcag ctccagcaac agccgcaccc
     4441 ccgccgcctc ggcatgagcc acgacgggcg cgagaccctc gacgcaggcg gcgatcgccg
     4501 gttggtccgc gccgtcgccg atgcggtcac ccgagaagac gatcaccgcc tcgcatccgc
     4561 cggccgcggc cgtgtcgatg ttgcggcgga cctcgtcggc cagggccgcg tgcagcgacc
     4621 ggtcgttgaa cccgcgctcc agcgtgtggc cggtgagggt gacgatggac aggcccacgt
     4681 cctgggccac aggccagagc gactctggca acatctcgac cccttgggcg ccggccgcgg
     4741 ccgcctcgcg cagcaaagtc tcgggttcga tttcgcggcc gagggtgaag gaccaccagg
     4801 cgaagctctg ccggatcatt cgcccgcccc gctcaatgat gtccaacggc cgttggccgc
     4861 cgcgctacgc accgccgcct cgatgaatcg cacgccctcg accccggccg tgacgccggg
     4921 caggtcggtc gcg
//
LOCUS       DAORNX010000204         4926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12751_length_4926_cov_7.227058, whole genome shotgun sequence.
ACCESSION   DAORNX010000204 DAORNX010000000
VERSION     DAORNX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_4926_cov_7.227058"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PLO65_09725"
     CDS             complement(<1..566)
                     /locus_tag="PLO65_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRD28565.1"
                     /translation="MSIEILMPALSPTMEEGTLAKWHVKAGDTVKAGDVIAEIETDKA
                     TMEVEAVDEGVVEALLVDAGTEGVKVNAPIARLAGEGDDASFDTPAAPAAQDDVKGGP
                     APVAKAESTPPRHPEQAQGAVSKDATAAPAPKLNGSGGERVFASPLARRLAQAAGIDL
                     KSLKGSGPHGRVIKADVDAARGKAPAAAP"
     gene            complement(567..968)
                     /locus_tag="PLO65_09730"
     CDS             complement(567..968)
                     /locus_tag="PLO65_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD28566.1"
                     /translation="MTAAFISHTGGCHCGAVRFEADLPAVVEAQTCNCSMCEKVGFIH
                     IIVPESRFRLTAGADQITTYSFNTGVAQHTFCKVCGVKSFYRPRSNPDGWSVNARCLD
                     TTEGLDLRIEAFDGRNWEVHAAALAHLSKED"
     gene            complement(1080..2483)
                     /locus_tag="PLO65_09735"
     CDS             complement(1080..2483)
                     /locus_tag="PLO65_09735"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRD28567.1"
                     /translation="MSSVEILMPALSPTMEEGTLAKWHVKEGDTVKAGDVIAEIETDK
                     ATMEVEAVDEGVVGAILVPAGTENVKVNTPIARLGEGASFDTPAPQAAQDDVEGRSTP
                     AAKIDATPPRHGEQGAAAPVSNHAPTVELQDPEIPAGTKMVRITVRDALRDAMAEEMR
                     RDGDVFLMGEEVAQYQGAYKVSRELLQEFGDKRVIDTPITEHGFAGLGVGAAMAGLKP
                     IVEFMTWNFGMQAIDQIINSAAKTLYMSGGQIRAQIVFRGPNGAASRVGAQHSQDYSA
                     WYAQVPGLKVVAPYDAADAKGLLKAAIRDPNPVVFLEHEMMYGVEFDIPEVEDWVVPI
                     GKAKVRREGTDVTITAHSRMVGLALKAAEALAAEGINAEVIDLRTLRPLDHETILESV
                     RKTNRLVSAEEGWGPMGVGAEIVARVVEHAFDYLDAPPLRVHQEDVPLPYAANLEALS
                     LPGPDKIVKAVKAVCYR"
     gene            complement(2480..3496)
                     /gene="pdhA"
                     /locus_tag="PLO65_09740"
     CDS             complement(2480..3496)
                     /gene="pdhA"
                     /locus_tag="PLO65_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRD28568.1"
                     /translation="MARTPKAESVSKGTSVAAGKARLLKHYRDMLLIRRFEERAGQLY
                     GMGLIGGFCHLYIGQEAIAVGMEAIRKPGDQIITGYRDHGHMLAAGMDPNEVMAELTG
                     RRGGSSKGKGGSMHMFSTAAGFYGGHGIVGAQVSLGTGLALADKYRGDGAVSFTYFGD
                     GAANQGQVYESFNMAELWSLPVVYVVENNQYAMGTSIERSSSETRLHKRGVSFNIPGE
                     EVDGMDVEAVIAAGAKAAEHARSGKGPYILEMKTYRYRGHSMSDPAKYRTKEEVDEVK
                     KTRDPIEHTRERLVALKVTDDEFKAIDSEVKTIVAAAAEFARTSPEPDPSELYTDVYL
                     EAAQ"
     gene            complement(3574..3870)
                     /locus_tag="PLO65_09745"
     CDS             complement(3574..3870)
                     /locus_tag="PLO65_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRD28569.1"
                     /translation="MFQRLQPLLPTFALLGLIVYFVFHGLTGERGLLLGSQRRALLAE
                     RTRELADLHSRRVDLEARARLLRDGSLSADLLEERARSLLGFADPKDYVIRTKP"
     gene            complement(4105..>4926)
                     /locus_tag="PLO65_09750"
     CDS             complement(4105..>4926)
                     /locus_tag="PLO65_09750"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD28570.1"
                     /translation="AHADNPIDIQEFMILPTGAATFSEGLRMGAEIFHGLKKALKDAG
                     HNTNVGDEGGFAPNIGSAEEALAFIVKAGEAAGFRAGEDFVLGLDVAATEFFKNGKYV
                     LEGEGKTVDPAGMVAYLEALVGKFPIVTIEDGCSEDDFEGWGLLTAALGKKIQLVGDD
                     LFVTNPERLSMGIDKGLANSILVKVNQIGTLSETLDAVDMAHRAGYTAVMSHRSGETE
                     DSTIADLAVATNCGQIKTGSLARSDRLAKYNQLLRIEEQLDDQAIYAGRRAIKQI"
BASE COUNT          717 a         1634 c         1689 g          886 t
ORIGIN      
        1 ggcgcggcgg cgggcgcctt gccgcgggcg gcgtcgacgt cggccttgat cacccggcca
       61 tgcgggccgg agcccttgag gctcttgaga tcaatgccgg cggcctgggc caggcggcgg
      121 gccagcggcg aggcgaacac gcgctcgccg ccggaaccgt tgagtttggg agccggcgcc
      181 gccgttgcgt ccttcgacac ggcgccttgc gcctgctcag gatgacgtgg aggggtgctt
      241 tcggctttgg cgacgggggc aggccctccc tttacgtcat cctgagcagc cggcgcagcc
      301 ggcgtgtcga aggacgcatc atcaccctcc ccggccagcc tggcgatagg cgcgttgacc
      361 ttcacgccct cggtgccggc gtcgaccagc agggcctcga ccacgccctc gtcgaccgcc
      421 tcgacctcca tggtcgcctt gtcggtttcg atctcggcga tcacgtcgcc ggctttcacc
      481 gtgtcgcccg ccttcacgtg ccatttggcc agcgtcccct cctccatggt cggggacagg
      541 gcgggcatga ggatctcgat ggacattcag tcttccttac tgagatgcgc cagggcggcc
      601 gcgtggacct cccagttgcg gccatcgaag gcttcgatgc gcagatcgag gccttcggtg
      661 gtgtcgaggc agcgggcgtt gaccgaccag ccgtccggat tggagcgggg ccggtagaag
      721 ctcttcacgc cgcagacctt gcagaaggtg tgctgcgcga cgccggtgtt gaagctgtag
      781 gtcgtgatct ggtcggcgcc ggccgtgagg cggaaccggc tctccggcac gatgatgtgg
      841 atgaagccga ccttctcgca catcgagcag ttgcaggtct gggcctcgac gacggccggc
      901 aggtccgcct cgaagcggac agcgccgcag tggcagccgc cggtgtgtga gatgaacgcg
      961 gcggtcattc taacctccga tcatccccgc gaaggcgggg acccaggcga ggtcagcggc
     1021 ggccgaggga ccgcttggat tcccgccttc gcgggaacaa tcggttcgag agcggcacat
     1081 caccggtaac aaaccgcttt caccgccttc acgatcttgt ccggaccggg cagcgacagg
     1141 gcttccagat tggccgcata gggcagcggg acgtcttcct ggtgaacccg cagcggcggc
     1201 gcatcgaggt agtcgaaggc gtgctcgacg acccgggcga cgatctcggc gccgacgccc
     1261 atcgggcccc agccttcctc ggcgctgaca aggcggttgg tcttgcgcac gctctcgagg
     1321 atggtctcgt ggtcgagcgg acgcagggtg cgcagatcga tgacctcggc gttgatgcct
     1381 tcggcggcca gcgcctcggc ggccttcagc gccagcccga ccatccggct gtgggcggtg
     1441 atggtcacat ccgtgccctc gcggcggacc ttcgccttgc cgatgggcac gacccagtcc
     1501 tcgacctcgg ggatgtcgaa ctccaccccg tacatcatct cgtgttcgag gaagacgacc
     1561 gggttcgggt cgcggatggc ggccttgagc aggcccttgg cgtcggccgc gtcgtagggc
     1621 gcgacaacct tcaggccggg gacctgggcg taccaggcgc tgtagtcctg gctgtgctgg
     1681 gcgccgacgc gactggccgc gccgttgggg ccgcggaaga cgatctgcgc gcgaatctgg
     1741 ccgccggaca tgtagagggt cttggcggcg gagttgatga tctggtcgat ggcctgcatg
     1801 ccgaagttcc aggtcatgaa ctcgacgatc ggcttgaggc ccgccatggc cgcgccgacg
     1861 ccgaggccgg cgaagccgtg ctcggtgatc ggggtgtcga tcacccgctt gtcgccgaac
     1921 tcctgcagca gctcccggct gaccttgtag gcgccctggt actgggcgac ctcctcgccc
     1981 atcaggaaca catcgccgtc gcggcgcatc tcctcggcca tggcgtcgcg cagggcgtcg
     2041 cggaccgtga tgcggaccat cttggtcccg gcgggaattt cggggtcctg aagctcgacc
     2101 gttggtgcgt ggttcgacac gggcgctgcc gcgccctgct caccatgacg tggaggggtg
     2161 gcgtcaattt tggcggcggg agttgaccta ccctctacgt catcctgagc agcctgcgga
     2221 gcaggcgtgt cgaaggacgc accctccccc agccgcgcga tcggcgtgtt gaccttcacg
     2281 ttctcggtgc cggcgggcac gaggatggcg ccgacgacgc cctcgtcgac ggcctcgacc
     2341 tccatggtcg ccttgtcggt ctcgatttcg gcgatcacgt cgccggcctt gaccgtgtcg
     2401 ccctctttca cgtgccactt ggcgagggtg ccctcctcca tggtcgggga gagggccggc
     2461 atgagaatct cgaccgaact cactgcgcgg cctccaggta gacgtcggtg tacagctcgg
     2521 acgggtccgg ctcggggctg gtgcgggcga actcggcggc ggccgcgacg atggtcttca
     2581 cctccgagtc gatggccttg aattcgtcgt ccgtgacctt cagggcgacc aggcgttcgc
     2641 gtgtatgttc aatgggatcg cgggtcttct tgacctcgtc gacctcctcc ttggtgcggt
     2701 atttggccgg gtcgctcatc gagtggccgc ggtagcggta ggtcttcatc tcgaggatgt
     2761 agggcccctt gccgctgcgg gcgtgctcgg cggccttggc gccggcggcg atgaccgcct
     2821 cgacatccat gccgtcgacc tcctcgccgg gaatgttgaa cgacaccccg cgcttgtgca
     2881 gccgggtttc cgaactcgac cgctcgatcg aggtacccat ggcgtactgg ttgttctcga
     2941 cgacgtagac gaccggcagg ctccacagct cggccatgtt gaagctctcg tagacctggc
     3001 cctggttggc cgcgccgtcg ccgaagtagg tgaagctgac cgcgccgtcg ccgcggtact
     3061 tgtcggccag cgccagaccc gtgccaaggc tgacctgggc cccgacgatg ccatggccgc
     3121 cgtagaagcc cgcggcggtc gagaacatgt gcatcgagcc gcccttgccc ttcgacgagc
     3181 cgccgcgacg gcccgtcagc tcggccatca cctcgttcgg gtccatgccg gcggccagca
     3241 tgtggccgtg gtcgcgatat cctgtgatga tctggtcgcc gggcttgcgg atcgcctcca
     3301 tgcccacggc gatcgcttcc tggccgatgt agaggtggca gaagccgccg atcaggccca
     3361 tgccgtacag ctggccggcc cgctcctcga agcggcggat caagagcatg tcccggtagt
     3421 gcttgagcag ccgggccttg ccggccgcga cggacgtccc tttcgaaacg ctttcggcct
     3481 taggcgtccg cgccatcgac ttctcccttg aaccgtatca tcgcggccag atcgcccctc
     3541 gcttcgcagc cgggacgaaa gattcgaccc ggatcagggc ttggtgcgga tcacatagtc
     3601 tttcggatcg gcgaagccca gcaaggaacg cgcacgctcc tcgagaaggt ccgccgacag
     3661 gctgccgtca cgtaatagcc gggcccgggc ctcgaggtcc accctgcggc tgtgcagatc
     3721 ggcgagctcg cgcgtccttt ccgcgagcag cgcccggcgt tgcgacccga gcagcaaccc
     3781 acgctccccg gtcaggccgt ggaacacgaa atagacgatc agcccgagca gggcgaaggt
     3841 cggaagaagg ggctgcaggc gttggaacac gctggcgaat ccgggcgatg gtgcgaggcc
     3901 agcttgggtc gggcgtggtt aagggggcgc taatcttcca taccttgctc cccacgtgtg
     3961 gggagctgtc aggcggaagc tgactgaggg gcggcgccgg cgtcaggcgg gcgagtcaga
     4021 acgcggacaa tcaccacgct ccgcgccgcc ccctccacca cgctccgcgt ggtccccctc
     4081 cccggcttcg cggggaggat tgacctagat ttgtttgatc gcccgccggc cggcgtagat
     4141 cgcctggtcg tccagctgtt cctcgatgcg cagcagctgg ttgtacttgg ccagccggtc
     4201 ggagcgggcc aaggaaccgg tcttgatctg cccgcagttg gtggcgacgg cgaggtcggc
     4261 gatggtcgag tcctcggtct cgcccgaacg gtggctcatc accgccgtgt agccggcgcg
     4321 atgggccata tcgacggcgt cgagcgtctc actcagcgtg ccgatctggt tgaccttgac
     4381 caggatcgag ttggcgaggc ccttgtcgat gcccatcgac aggcgctcgg ggttggtcac
     4441 gaacaggtcg tcgccgacca gctggatctt cttgccgagg gcggccgtga gcaggcccca
     4501 gccctcgaag tcgtcttccg agcagccgtc ctcgatggtc acgatcggga acttgccgac
     4561 cagggcctcg agataggcga ccatgccggc cggatcgacg gttttgcctt caccttcaag
     4621 cacatatttg ccgttcttga agaactcggt ggccgccacg tcgagaccga gcacgaaatc
     4681 ctcgccggcg cggaagccgg ccgcctcgcc cgccttgacg atgaaggcca gcgcctcctc
     4741 ggccgagccg atgttcgggg cgaagccgcc ctcgtcgccg acgttggtgt tgtggccggc
     4801 gtccttgagg gccttcttca ggccgtggaa gatctccgcc cccatgcgca ggccttcgga
     4861 gaaggtcgcc gcgccggtcg gcaggatcat gaactcctgg atgtcgatcg gattgtcggc
     4921 gtgggc
//
LOCUS       DAORNX010000205         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12815_length_4907_cov_8.684872, whole genome shotgun sequence.
ACCESSION   DAORNX010000205 DAORNX010000000
VERSION     DAORNX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12815_length_4907_cov_8.684872"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLO65_09755"
     CDS             <1..1638
                     /locus_tag="PLO65_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28571.1"
                     /translation="VGSLIAAPGVRSASLIDHQGRILAVQRAPGAGEGAETTVRTPVM
                     VAGQAVGSLITTVEAPRLSALLPRYLALTFALFFGAAGIAMFVANALARRVIRPVSQL
                     SEAMGEVAESGRFTPVAEDADDDLFRSLAQSFNRLLAKLDANDRELRQTMAELVDARD
                     MANTANILKSQFLANMSHEIRTPLNGVLAMADVMARGELDDRQRERLGIIRESGELLL
                     SVINDVLDLSKIEAGRLELAEHAFSLDEVAEGAVSAFSVLAAQKSLRFGMTVDAEVAG
                     WWLGDSDRIRQVLSNLLSNAVKFTVEGAVAARFSASPSGGLRLTVSDTGIGIAGDKLP
                     TLFEKFIQADNSTTRRFGGTGLGLAICRELAQLMGGSISARSIEGEGSTFTVDLPLQR
                     AAAPAEEATPDSALEALEERKLRILAAEDNATNQKVLQAVLEPLDVHLHIVPDGKQAV
                     DAWRRGGFDLILMDVQMPVMDGVEAARTIRAAEAAEGGPRIPILALTANALVHQVESY
                     LAAGMDGHVAKPIELTRLYDAIETTLANAAAARSKAA"
     gene            1756..2166
                     /locus_tag="PLO65_09760"
     CDS             1756..2166
                     /locus_tag="PLO65_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28572.1"
                     /translation="MKKLLIPLLAASTLAGAALPAAAAPWMNINQRQAQLDQRIDMGV
                     RNGSLTRREAMNLRAEFRGIANLEARYRRTGGLQAWERRDLDRRFNALSARIRYERHD
                     RDYRGRPGPGPAYDTDRDGHPDRVDRFPRDPRRW"
     gene            complement(2236..3048)
                     /locus_tag="PLO65_09765"
     CDS             complement(2236..3048)
                     /locus_tag="PLO65_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRD28573.1"
                     /translation="MRQWTVDAFASGPFRGNPACVVEPMAAWRDAAWMQALAAENNQA
                     ETAFLLTTDEPARFGLRWFTPALEVPLCGHATLAAAHVLFAELGVAADRITFDTQSGP
                     LAVKRLGERYEMDFPASVPKRTGVPEGLVEALGATPAEVWAGSYLVAVFDSEAAVRAL
                     KPDLAALEKIAGEATGGRGNLSAAALADAGSDYDVVSRFFAPGSGIPEDPATGSAHCI
                     LSPLFAGRLGRADLRFHQAYPGRGGDLDCEHRGERVLLRGKAVTVIDSVLRL"
     gene            3092..3547
                     /locus_tag="PLO65_09770"
     CDS             3092..3547
                     /locus_tag="PLO65_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28574.1"
                     /translation="MTFELNGGKLLGLFAPIWALLALSSLVNPAAWVADPANVALAAG
                     IPFAASGLLWAVGRRRWRLELTPTALIHHTLGRTETFDWARMGPVGVSRVIGLDLVSP
                     STLHFSYAGEGLSGVAGRLVGRRLLCVFGDGSAGRLLRQIEDYRRLHGV"
     gene            3557..4012
                     /locus_tag="PLO65_09775"
     CDS             3557..4012
                     /locus_tag="PLO65_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28575.1"
                     /translation="MPVETRTETGYLVSDAPARFDLPLAHRWISEQSYWGKHIPYLTF
                     ATAVRNSLTFAAYAPDGAMAAMARVVTDRATFGWLCDVFVDEAHRGRGLGKALMACVK
                     AHPDLQGLRRLHLATADAHELYRQFGFTELTGVDRWMEIRDPDLYTRGR"
     gene            complement(4097..4675)
                     /locus_tag="PLO65_09780"
     CDS             complement(4097..4675)
                     /locus_tag="PLO65_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD28576.1"
                     /translation="MSQSLEHRLEDILRAAPSMMTVLEHARDLDLPDWLIVSGAVYQR
                     VWNHLTGREPDYGVRDYDVVYFDPDTSWDAEDVFIRRAAALFPAPLNDLVEVRNQARV
                     HLWFEDHFGEPYEPLSSSAEALSRFVAPAFGVGVRLESDDSLTIRAPFGLEDLFAMTL
                     RPNPNRPLARGWTKTTASARARWPEVTVVEVV"
     gene            complement(4672..>4907)
                     /locus_tag="PLO65_09785"
     CDS             complement(4672..>4907)
                     /locus_tag="PLO65_09785"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRD28577.1"
                     /translation="MTADGETSFEGERIDTRHTHGTGCTLASACAVGLAQGLPPGAAV
                     ARAWDYVHQAMLRAPGFGAGHGPLDHGWPMRGQ"
BASE COUNT          728 a         1763 c         1701 g          715 t
ORIGIN      
        1 gtcggatcgc tgatcgccgc gcccggcgtg cgctcggcca gcctgatcga tcaccagggc
       61 cggatcctcg ccgtgcagcg cgccccgggc gccggcgaag gcgccgagac gaccgtgcgc
      121 acccccgtca tggtggcggg tcaggccgtc ggatcgctga tcaccaccgt cgaggcgccg
      181 cggctgtcgg ccctgttgcc gcgttacctg gccctgacct tcgccctgtt cttcggcgcg
      241 gcgggaattg cgatgttcgt cgccaacgcg ctggcgcgac gcgtgatccg gccggtcagc
      301 cagctgtcgg aggccatggg ggaggtggcc gagagcggcc gcttcacgcc ggtggccgag
      361 gacgccgacg acgacctgtt ccgcagcctg gcccagagct tcaaccggct gctggccaag
      421 ctcgacgcca acgaccgcga gctgcgccag acgatggccg agctggtcga cgcccgcgac
      481 atggccaaca cggccaacat cctcaagtcg cagttcctcg ccaacatgag ccacgagatc
      541 cgcacgccgc tgaacggcgt gctggcgatg gcggacgtca tggcgcgcgg cgagctcgat
      601 gatcgccagc gcgaacggct gggaatcatt cgcgagtccg gcgaactgct gctcagcgtg
      661 atcaacgacg tgcttgacct gtcgaagatc gaggccggcc ggctcgaact ggcggagcac
      721 gccttctcac tggacgaggt ggccgagggc gcggtcagcg ccttctccgt gctggccgcg
      781 cagaagtcgc tgcgcttcgg catgaccgtc gacgccgagg tggccggctg gtggctcgga
      841 gactccgatc gcatccggca ggtgctcagc aacctgctgt cgaacgcggt caagttcacg
      901 gtcgaaggcg cggtagccgc ccgcttctcg gcctcgccga gcggcgggct gcggctgacc
      961 gtgtcggaca ccggcatcgg catcgccggc gacaagctgc cgaccctgtt cgagaaattc
     1021 atccaggccg acaactcgac cacgcgccgc ttcggcggga ccggcctggg cctggccatt
     1081 tgccgcgaac tggcccagct gatgggcggt tcgatctccg cccgcagcat cgagggcgag
     1141 ggctcgacct tcaccgtcga tcttccattg cagcgcgccg cagcgccggc cgaggaggcg
     1201 acgccggact cggcccttga ggcgcttgag gaacgcaagc tgcgcatcct cgccgccgag
     1261 gacaacgcca ccaaccagaa ggtgctgcag gccgtgctgg agccgctcga cgtgcacctg
     1321 cacattgtgc cggacggcaa gcaggccgtc gacgcctggc gccggggcgg cttcgacctg
     1381 atcctgatgg acgtgcagat gccggtcatg gacggggtcg aggcggcgcg aacgatccgc
     1441 gcggcggagg ccgccgaggg cggaccgcgg atcccgatcc tggccctgac ggcgaacgcc
     1501 ctggtgcacc aggtcgagtc ctacctggcc gccggcatgg acggacacgt cgccaagccg
     1561 atcgagctga cccgcctcta cgacgcgatc gagacgaccc tggccaacgc ggccgcggcg
     1621 cgctccaagg cggcctgacg gccgatcgaa tcctgaacgt cagacaacga gcggctcaga
     1681 accgtttcag gtcccacgtg ttttcttgtg gccatggtcg tcgccaaggc ggcttgagca
     1741 acgaggaact ccccgatgaa aaagcttctg atccccctcc tggcggcctc gactctggcc
     1801 ggcgccgccc tccccgccgc cgctgcgccg tggatgaaca tcaaccagcg tcaggcccag
     1861 ctcgaccagc gcatcgatat gggcgtccgc aacggctccc tgacccgccg cgaggcgatg
     1921 aacctgcgcg ccgagttccg cggcatcgcc aatctcgagg cccgctatcg ccggaccggc
     1981 ggcctgcagg cctgggagcg tcgcgacctc gaccgtcgct tcaacgccct gagcgccagg
     2041 atccgctacg agcggcacga tcgtgactac cgcggccgtc cgggtcccgg cccggcctat
     2101 gacaccgacc gcgacggtca tcccgaccgg gtcgaccggt tcccccgcga cccgcgccgc
     2161 tggtaggctc ccccggacca gcgataacgc gaaggaacgc cccggcttct ccccgagccg
     2221 gggcgttttt cgtagtcaga gccgcaggac gctgtcgatc accgtgacgg ccttgccgcg
     2281 cagcagaacc cgctcgccgc ggtgctcgca gtcgaggtcg ccgccgcggc ccggataggc
     2341 ctggtggaac cgcaggtcgg cgcggcccag cctgcccgcg aacagaggtg agaggatgca
     2401 gtgggccgag cccgtggccg gatcctcggg gatgcccgag ccgggcgcga agaagcggct
     2461 gaccacatca tagtccgaac ccgcgtccgc cagcgccgcc gccgacaggt tgccgcgtcc
     2521 gcccgtggcc tcgccggcga tcttttccag cgccgccaga tcgggcttca gcgccctgac
     2581 cgccgcctcg ctgtcgaaca ccgccaccag atagctgccg gcccagacct ccgccggcgt
     2641 cgcgccgagc gcctcgacca ggccttcggg cacgccggtc cgcttcggaa cgctggccgg
     2701 aaagtccatc tcgtagcgct cgccgagacg cttgacggcc aggggacccg actgcgtgtc
     2761 gaaggtgatc cggtcggccg cgacgcccaa ctcggcgaac agcacatggg ccgcggccag
     2821 ggtcgcgtga ccgcacagcg gcacctccag ggccggggtg aaccagcgca ggccgaaccg
     2881 cgctggctcg tccgtggtca gcagaaaggc cgtctcggcc tggttgttct ccgccgccag
     2941 ggcctgcatc caggccgcat cgcgccaggc ggccatcggc tcgacgacgc aggcgggatt
     3001 gccccggaag gggccggagg cgaacgcgtc gacagtccac tggcgcatga gggattcccc
     3061 ggataacggc aaaacgcgat aggtgggcgt catgacgttt gaactcaatg gcggcaagct
     3121 tctggggctg ttcgcgccga tctgggcgct gctggcgctg tcctcgctgg tcaatccggc
     3181 cgcctgggtc gccgatcccg caaacgtcgc cctggccgcg gggattccgt tcgcggcgtc
     3241 cgggctgctg tgggcggtcg gccggcggcg ctggcgactg gagctgacgc ccacggccct
     3301 gatccaccac acgctcgggc gcacggagac gttcgactgg gcgcggatgg ggccggtggg
     3361 cgtgagccgc gtcatcggcc tcgacctggt gtcgccctcg accctgcatt tcagctatgc
     3421 cggcgagggc ctgtcgggcg tcgcgggtcg gctggtcggc cgccggctgc tgtgcgtctt
     3481 cggcgacggc agcgccggca ggctgctgcg ccagatcgag gactaccgcc gcctgcacgg
     3541 cgtctgagga ggagccatgc ccgtcgaaac ccgcaccgag accggctatc tcgtcagcga
     3601 cgcccccgcc cggttcgacc tgccgctggc ccatcgctgg atcagcgagc agtcctactg
     3661 gggcaagcat atcccctacc tgaccttcgc cacggcggtc cgcaacagcc tgaccttcgc
     3721 ggcttacgcg cccgacggcg ccatggcggc catggcccgg gtggtcaccg accgggcgac
     3781 cttcggctgg ctgtgcgacg tgttcgtcga cgaggcccat cggggccggg gtctcggcaa
     3841 ggcgctgatg gcctgtgtga aggcccatcc ggacctgcag ggcctgcgcc gcctgcatct
     3901 ggccaccgcc gacgctcatg agctctaccg tcagttcggc ttcaccgaac tgaccggcgt
     3961 cgatcgctgg atggagatcc gcgatccgga cctctacacg cgcgggcggt gatggacggg
     4021 gacatgtacc gccttcggta cgtgtcccca gcagcttgca gcggcagggg acacgcactg
     4081 aagtgcatgt ccccgctcag acgacttcca ccaccgtcac ctccggccag cgcgcgcggg
     4141 ccgaggcggt ggtcttggtc cagcccctgg ccagcggccg gttggggttc ggccgcaggg
     4201 tcatggcgaa cagatcctcc agcccgaacg gggcccggat ggtcaggctg tcgtcggact
     4261 ccagccgcac cccgacgccg aacgcgggag ccacgaaccg gctcagcgcc tcggcgctgc
     4321 tcgacagcgg ttcgtagggc tcgccgaagt ggtcctcgaa ccacaggtgc acacgggcct
     4381 ggttgcgcac ctccacgagg tcgttgagcg gcgccgggaa cagggccgcg gcgcgcctga
     4441 tgaacacatc ctctgcgtcc cagctggtgt cggggtcgaa atagaccacg tcgtagtcgc
     4501 gcacgccgta gtcgggctcg cggccggtca ggtggttcca cacccgctgg tagacggcgc
     4561 cggagacgat cagccagtcg ggcaggtcca ggtcgcgcgc gtgctcgagc accgtcatca
     4621 tcgagggcgc cgcgcgcagg atgtcttcca gccggtgttc gaggctttgg ctcattgtcc
     4681 cctcatcggc cagccatgat cgagcggccc gtggccggcg ccgaagccgg gcgcgcgcag
     4741 catggcctgg tggacataat cccacgcccg ggccacggcg gcgcccggcg gcaggccctg
     4801 ggcgaggccg acggcgcagg cgctggccag ggtgcagccc gtgccgtggg tgtgtcgcgt
     4861 gtcgatccgc tcgccctcga agctggtctc gccgtcggcg gtcatca
//
LOCUS       DAORNX010000206         4896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12847_length_4896_cov_6.105144, whole genome shotgun sequence.
ACCESSION   DAORNX010000206 DAORNX010000000
VERSION     DAORNX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12847_length_4896_cov_6.105144"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLO65_09790"
     CDS             complement(<1..192)
                     /locus_tag="PLO65_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRD28578.1"
                     /translation="MVDLSAEMAELWASLGGTSPGRARAVQFVAARRGEGTSTVAREF
                     AAYAASRAGRTVWLVDLDLF"
     gene            complement(253..1755)
                     /locus_tag="PLO65_09795"
     CDS             complement(253..1755)
                     /locus_tag="PLO65_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRD28579.1"
                     /translation="MSSQSAWRTIPHDPPAAAGWASRPRYAPSDFITLLWRERGLMLG
                     VFLVILALGVVFAMTLKKAYSAESSILIQLGQEYVYEPRAGDAGRGAVPDTDSLVQSE
                     VEILQSGQLRERVLRRIGLGVVYPDLAEKYAAASPADKAVLMAKAIDSMGRKLSVGSA
                     PANPVVRVSFEHDDPVMAARVVNTLLEEYLVYRRSVLIHPTSPVFERQRVMFEEQLQQ
                     ADAAYQDFLTTNDIGDFAAQKTAATQLIGQIEAQRYAAEVTLQDRQARLAVLDGQLAQ
                     VTPEIGLYRDIDASASSRLAALRLQREEMLSRYRPEADPVRDIEAQIAQLEAGVSAGR
                     TATDGARRIGVNPVHQTLQTERIQTAAEIAALQQSLSAYAAQAAKATEQLQRLATLEP
                     QFQALTMDRDVLQSSVRDFTVRERQDQASRQIASESNDNIRIVDRATPATKGKSLRKP
                     VAMLAFLFGAFSALCAGLVRMFLRPGLPTRASAQRTLDLPVLGTAPVKLR"
     gene            1843..2622
                     /locus_tag="PLO65_09800"
     CDS             1843..2622
                     /locus_tag="PLO65_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRD28580.1"
                     /translation="MRRTAPPRVALMLTAALAVGSPAQAQWWPFGGARGDRADPPPPR
                     PAAPSFPNIDYADWSDREPDYRLYPGDQIDITVPSAPELSKQAVVVQPDGRVSMPLIA
                     PVMAADRTVPELEASLGRAYASQLLRPQVSVTIKAATPLKVFVGGEVGAPGVYDMAGD
                     INSLQAVIQAGGFKPSAKTDKVVIIRRGQGGQAMMRTVNLESAMKAPGGADFVPLRRF
                     DIVYVPRSNISEAGLWVQQWFRDLSPVQFGFSYALNGQNVN"
     gene            complement(2630..2905)
                     /locus_tag="PLO65_09805"
     CDS             complement(2630..2905)
                     /locus_tag="PLO65_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HRD28581.1"
                     /translation="MLASVEIEAPATMPLPGPDSSGDELFRMGLLYSTGQGGAPLDYV
                     SAHMLFNLAAMRGSVEAKIYRKELSAEMDPADVADAQRQARQWLAQG"
     gene            complement(3077..3814)
                     /locus_tag="PLO65_09810"
     CDS             complement(3077..3814)
                     /locus_tag="PLO65_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD28582.1"
                     /translation="MTDNANRLLGALRAADLERLSPRLTACDFEPGHVLHRAGDAVRY
                     AWFPCGASLATFVVTLEPGVIVEAAMVGREGAIGGIVSHGRLPAYADVQVLHGGPFLR
                     IDAAGLEDIKGQSPAVANLLARYADCLLAQMLQSVACNAGHTITQRTAKWLTAAIDRT
                     GDHVVPLRQDQLADMLGVGRSFVNRVLASLRESGVLTTRRGSLLIDDMTALKALACPC
                     NDRLRDHFEAVLAGVYPDPADPEAAPS"
     gene            3957..>4896
                     /locus_tag="PLO65_09815"
     CDS             3957..>4896
                     /locus_tag="PLO65_09815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD28583.1"
                     /translation="MPRPAEPPARWWPLLDGRRVGEIIPRRQPGLAACLGLTFACVGA
                     AGLFRLTIDPLVGQQVALATFLAPAALCAFMLGFGWGTLAAALGGGVALLLLVRGSGD
                     GPAVGDLWSLSAFALIATIIVSLMALARSALLTARAERANTEAVNERLQLVSQELSHR
                     IQNIFAVVAGMVTLSVREHPAATAYAEDLLARVRALGSAHDLVRPHGPGGRQADCAMT
                     FSALLDVLFLAYRAAGAERIVVSGADMRLEPAAATPVALLFHELATNSLKYGALSRAD
                     GGVSVHATRRGEDVEIVWTETGGPPLQAPPRHSGFGS"
BASE COUNT          729 a         1724 c         1700 g          743 t
ORIGIN      
        1 gaaaaggtcc agatcgacca gccagaccgt gcgcccagcg cggctggcgg cataggcggc
       61 gaactcgcgg gccacggtcg aggtcccctc cccgcggcga gcggcgacga actgcacggc
      121 gcgcgcgcgg ccgggcgacg tcccgccgag cgacgcccac aactccgcca tctcggcgct
      181 caaatccacc atcgctcgaa tcgccgcgcg cccaatccca tgcagggtag actattgtcg
      241 ggcccctgag acctagcgca gcttgaccgg cgcggttccg agcacaggaa ggtcgagggt
      301 gcgctgggcg gaagcccgcg tcggcagccc cggccgcagg aacatcctca ccaggccggc
      361 gcagagcgcg gagaaggccc cgaacaggaa ggccagcatg gccaccggct tgcgcaggct
      421 cttgcccttg gtcgcgggcg tggcccggtc aacaatgcgg atgttgtcgt tgctctccga
      481 ggcgatctgc cgcgacgcct ggtcctgccg ctcgcgcacc gtgaagtctc gcacgctgct
      541 ttgcagcacg tcgcgatcca tggtcagggc ctggaactgc ggctcgaggg tggcgaggcg
      601 ctgcagctgt tcggtggcct tcgccgcctg ggcggcatag gccgacagcg attgctgcag
      661 ggcggcgatc tcggcggccg tctggatgcg ttcggtctgc agcgtctggt ggaccggatt
      721 gaccccgatg cggcgggcgc cgtcggtcgc ggtccggccg gcgctgaccc ccgcctccag
      781 ctgggcgatc tgggcctcga tgtcccgcac cgggtcggcc tctggccggt agcgcgacag
      841 catctcctcc cgctgcagcc ggagggcggc caggcgggac gaggccgagg cgtcaatgtc
      901 gcggtacagg ccgatctcgg gcgtgacctg cgccagctgg ccgtcgagca ccgccagacg
      961 ggcctggcga tcctgcagcg tgacttcggc cgcgtagcgc tgggcctcga tctgcccgat
     1021 cagctgggtc gcggcggtct tctgggccgc gaagtcgccg atgtcattgg tggtcaggaa
     1081 gtcctggtag gccgcgtccg cctgctgcag ctgttcctcg aacatgaccc gctggcgttc
     1141 gaacaccggc gaggtcggat ggatcagcac cgagcgccgg tagaccaggt actcctcgag
     1201 cagggtattg accacgcggg ccgccatcac cggatcgtcg tgctcgaacg agacccgcac
     1261 cacgggattg gccggggcgg agccgaccga cagtttgcga cccatggagt cgatggcctt
     1321 ggccatcagc accgccttgt cggccggcga cgccgccgcg tatttttccg ccaggtcggg
     1381 atagaccacg cccaggccga tacgacggag cacccgctcg cgaagctggc cgctctgcag
     1441 gatctcgacc tcggactgca ccagggaatc cgtgtccggc accgcgccgc gccccgcgtc
     1501 cccggcgcgc ggctcgtaga catactcctg gcccagctgg atcaggatgc tggattcggc
     1561 cgaataggcc ttcttgagcg tcatggcgaa gacgacgccc agggccagga tgaccaggaa
     1621 cacccccagc ataagcccgc gttcgcgcca cagcagggtg atgaagtcgc tcggggcata
     1681 gcgcggccgg gacgcccagc cggcggccgc cggcgggtcg tgtggtatgg tcctccaggc
     1741 gctttgcgag ctcatgcgaa tccgggactt tctggccttg tcccggaccc tcgccggagc
     1801 gcgattaaca ctccgttacc cattctcgtt aacggttgga tcatgcgtag aacggccccg
     1861 ccccgcgtcg ccctgatgct gaccgccgcc ctggccgtcg gatcgccggc gcaggcccag
     1921 tggtggccct tcggcggcgc gagaggggac cgggccgatc cgccgccgcc ccgacccgcg
     1981 gccccgagct ttcccaacat cgactatgcc gactggagcg atcgggagcc tgactaccgg
     2041 ctctatcctg gcgaccagat cgacatcacc gtcccgtcgg cgcctgaatt gtcgaagcag
     2101 gccgttgtcg tccagccgga cggccgggtg tccatgcccc tgatcgcgcc ggtgatggcc
     2161 gcggaccgga ccgtgccgga actcgaggcg tcgctcggcc gggcctacgc cagccagctg
     2221 ttgcggccgc aggtctcggt gacgatcaag gcggccacgc cgctgaaggt gttcgtcggg
     2281 ggcgaggtgg gcgcgccggg cgtctatgac atggccggcg acatcaacag cctgcaggcg
     2341 gtgatccagg ccggcggctt caagcccagc gccaagaccg acaaggtggt gatcatccgc
     2401 cgcggccagg gcgggcaggc gatgatgcgg acggtcaatc tcgagtcggc gatgaaggcg
     2461 ccggggggcg cggacttcgt gcccctgcgc cggttcgaca tcgtctacgt gccccggagc
     2521 aacatctccg aggccggcct gtgggtccag cagtggttcc gcgacctgtc cccggtgcag
     2581 ttcgggttca gctacgcgct caacggccag aacgtgaact agggtccggt cagccctggg
     2641 ccagccactg gcgggcctgg cgctgggcgt cggcgacgtc ggccggatcc atctcggcgc
     2701 tcagttcctt gcggtagatc ttggcctcga ccgaaccgcg catggccgcc aggttgaaca
     2761 gcatgtgggc cgaaacgtag tcgagcggcg ctccgccctg accggtcgag taaagcagac
     2821 ccatgcggaa cagctcgtcg cccgaggagt ccgggcccgg cagcggcatg gtcgcgggag
     2881 cttcgatttc gacgctcgca agcatggtct tcttcccttc aaatcccacc ccggacggtt
     2941 ctgtccggag ccgatgagag cagaagacta ccgggcggct gaacataggg ttaaccgcgc
     3001 aaatcctttg tattcaaggg cgaatacagg attcgttcat ccccgttact gcccgtaaaa
     3061 ctgtgtcgcc ggcaggttaa gacggggccg cctcgggatc ggccggatcg ggatagacac
     3121 cggccagaac ggcctcgaaa tggtcccgca ggcggtcatt gcagggacag gcgagcgcct
     3181 tgagcgcggt catgtcgtcg atcagcaagg agccgcgtcg cgtggtcagc acgccgctct
     3241 cgcgcagcga cgccagcacg cggttgacga aactcctgcc cacgccaagc atgtccgcca
     3301 gctgatcctg gcgcaggggc acgacatggt cccccgtgcg atcgatggcc gccgtcagcc
     3361 acttcgccgt ccgctgggtg atggtgtggc cggcgttgca ggccaccgac tggagcatct
     3421 gggccagcaa acagtcggcg tatcgggcca gaaggttggc gaccgccggg gactgaccct
     3481 tgatgtcctc cagacccgcc gcgtcgatgc gcaggaaggg accgccgtgc agaacctgga
     3541 catcggcata ggccggcagg cggccgtgcg agacgatgcc gccgatggcc ccttcccggc
     3601 cgaccatggc ggcctcgacg atcaccccgg gctccagcgt gaccacgaag gtggccagcg
     3661 acgcgccgca gggaaaccag gcgtagcgca ccgcatcccc ggcccggtgc agcacatggc
     3721 cgggttcgaa gtcgcaggcc gtcagacgcg gcgacagccg ttccagatcg gccgcccgca
     3781 gcgcgcccag cagccggttg gcgttgtccg tcatggcggt ctccagcatc ccgaggacag
     3841 caacgcccgg gacggcgtta tgttcactag tggacggacc ggtcgctcgc ggcgtgctac
     3901 cccgcgctcc taacgggctt ccttgccggt cgctgcgtcg agagaattcc acccccatgc
     3961 cgcgccccgc cgaaccgccc gcacgctggt ggcctctcct cgacgggcgc cgggtcggcg
     4021 agatcattcc ccggcgccag ccgggcctcg cggcctgcct cggcctcaca ttcgcctgcg
     4081 tcggcgccgc cggcctgttc cgcctgacga tcgatcccct ggtcggacag caggtcgccc
     4141 tggcgacctt cctggcgccg gccgccctgt gcgcattcat gctcggcttc ggctggggga
     4201 cgctggccgc ggccctcggc ggcggcgtcg ccctgctgct gctggtcagg ggcagcggcg
     4261 acgggccggc cgtgggagac ctctggtcgc tgagcgcgtt cgccctgatc gcgacgataa
     4321 tcgtcagcct catggccctc gcccggtcgg ccctgctgac ggcgcgcgcg gagcgggcca
     4381 acaccgaggc ggtcaatgag cggctgcagc tggtcagcca ggaactgagc caccggatcc
     4441 agaacatctt cgccgtcgtc gccggcatgg tgacgctgtc ggtgcgcgag cacccggccg
     4501 cgaccgccta cgccgaagac cttctggccc gcgtgcgcgc cctgggcagc gcccatgacc
     4561 tggtgcggcc gcacgggccc ggcggccgcc aggctgactg cgcgatgacc ttcagcgccc
     4621 tgctggatgt cctgtttctg gcctaccggg cggccggggc cgagcggatc gtcgtctccg
     4681 gcgccgatat gcggctggag ccggccgcgg ccacgcccgt ggcgctgctg ttccacgaac
     4741 tggcgaccaa ttccctcaag tacggcgccc tgtcgcgggc cgacggcggg gtgagcgtcc
     4801 acgccacccg ccggggcgag gacgtggaga tcgtctggac cgaaaccggc gggccgccgc
     4861 tgcaggcgcc gccgcgccat tcgggcttcg gcagcc
//
LOCUS       DAORNX010000207         4874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12928_length_4874_cov_8.085080, whole genome shotgun sequence.
ACCESSION   DAORNX010000207 DAORNX010000000
VERSION     DAORNX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12928_length_4874_cov_8.085080"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLO65_09820"
     CDS             <1..254
                     /locus_tag="PLO65_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRD28584.1"
                     /translation="PDPVALETYVRTLAAAAPNAEGVDIYGPADAPLAMVRGRRRKRF
                     LVRADRTVDLQGFLAAWRARARPPGSVRVVIDVDPYSFL"
     gene            complement(416..1213)
                     /locus_tag="PLO65_09825"
     CDS             complement(416..1213)
                     /locus_tag="PLO65_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRD28585.1"
                     /translation="MDSLLQLAADPSAWAALVTLVVMEIVLGIDNLVFISILSNKLPE
                     AQRPRARSIGLSLALLMRIGLLFGIAWLVALTQPVFDLGIKGPLNEDGHAAFETAFSW
                     RDLILITGGLFLLWKATKEIHHSVDPAPTGDVMDARNTAISNFGAAIVQILMLDLVFS
                     IDSILTAVGMTHHVPIMIIAVTIAVAAMFFAAGPLADFIHKNPTIVMLALGFLLMIGA
                     VLIADGFGVHVPKGYIYAAMAFSGLVEGLNMIARRARSKAPPAETLH"
     gene            1371..2462
                     /locus_tag="PLO65_09830"
     CDS             1371..2462
                     /locus_tag="PLO65_09830"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD28586.1"
                     /translation="MQDAANIRPLTSLRFFAALWVVLFHYWPKLDVAITPALVGKGYL
                     GVELFFVLSGFILCHVYLSAFGEGRFRYGAFLWNRLARVYPLHLATLAGMIALGVVAG
                     LAGVAIDSGILRWDALPANLMLVQAWGFAPAAAFNHPSWSISAEWFAYLSFPAFGLLA
                     WTMRARPWLYAAGTLAAIAILYPAFQVLTGESLTQATIAWGALRIVPCFALGCAMHSL
                     WRSGAVRSARMAAIGALLSGAALLAAAILGAPDMVTVATFGGLILSLAGLAQHGSTFG
                     SQAVFVYLGEISYSTYMICVPWKIIFVNAAARVLNLADEKLPWPVWIVFIVALVPLSA
                     ASYHLVEKPARERMKLMADSWRARRAVAA"
     gene            2536..3225
                     /locus_tag="PLO65_09835"
     CDS             2536..3225
                     /locus_tag="PLO65_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRD28587.1"
                     /translation="MRQRTKTVLGSLAAAVVISLAPTANAMADIYWQCVPFARLLSGI
                     QIYGDARTWWGQAVGKYDTGRLPRMGSVLCFKPTGRMQLGHVAVVSRLVTDRVIQITH
                     ANWSQIGGARGQIEKDVTVIDVSPVGDWSMVKVWYDPIRDLGTSVYPTYGFIYQNGQA
                     QQLAQTDPRYWSAQGVAMARTAATQTASTVGPIPSPLGIINQAADATDRIAALIAAAT
                     GQAPAQEDTAD"
     gene            3295..4254
                     /locus_tag="PLO65_09840"
     CDS             3295..4254
                     /locus_tag="PLO65_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRD28588.1"
                     /translation="MLRVLRCGGGGLQPVEAGPDWVLPEDVAWIDLTRPERDEELIAE
                     KALGVLLPTREEMAEIEVSSRLYQEDGASFMTAFVLVGADGDDPALEPVTFVLARGRL
                     ITIRYVEPRAFVAYAAQAERDADFCSGALEAFLGLLDALVDRIADILEKTSADVEVIS
                     GEIFRPSQRAPFKLILRRLGRSQSIAAKARSSLVSLARLLSFAALTEAIADSKEHRDR
                     LRSLQRDVQSLTDHSAYLTENVTFLLDSAVGLINTDQNEIMKVFSVWAVILMPPTLIG
                     SVYGMNFTHMPELGWVGGYPMALGLMVLTMIAPIWWFRRKGWL"
     gene            4333..>4874
                     /locus_tag="PLO65_09845"
     CDS             4333..>4874
                     /locus_tag="PLO65_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRD28589.1"
                     /translation="MSAAPSLIRALDFSAVKAVVEAHDRYLGGRSGGKRANLTYVDLS
                     NMDLGGCDLADADFTGARLATTNLNGANLKRANMFGCDLREADLRAADLSQADLRGAC
                     LRGANLAGANLAGCDLREGQIALQGGAQAFRILKHERKAGELDYAVLQGANLAGAQMT
                     GAHAISADFTEANLSGACLNG"
BASE COUNT          742 a         1710 c         1599 g          823 t
ORIGIN      
        1 gccccgaccc cgtggcgctg gagacctatg tgcggaccct ggccgccgcc gcgccaaatg
       61 ccgagggcgt cgacatctac ggcccggccg acgcgccgct ggccatggtc aggggccgcc
      121 gccgcaaacg gttcctcgtg cgggccgacc gcaccgtcga cctgcagggc ttcctcgccg
      181 cctggcgggc ccgcgctcgt ccgcccggct cggtgcgggt ggtgatcgac gtcgacccct
      241 acagcttcct gtagggcgtg ggtcacctgt cccatccact gtcatcccgg aagccgcgca
      301 gcggctatcc gggacccaga tcaagtctca tccgcctcac cgtgaagccg aacctgggtc
      361 ccggctctcc gctacgctac ggccgggatg acagtgaaag gtctgggcag ccttcctagt
      421 gcagggtttc ggccgggggc gccttcgacc gcgccctgcg ggcgatcatg ttcagaccct
      481 cgaccaggcc cgagaaggcc atggcggcgt agatgtaacc cttgggcaca tgcacgccga
      541 agccgtcggc gatcagcacc gccccgatca tcagcaggaa gcccagggcc agcatgacga
      601 tggtcgggtt cttgtggatg aagtcggcca gcgggccggc cgcgaagaac atcgccgcca
      661 cggcgatggt cacggcgatg atcatgatcg gcacgtgatg ggtcatgccg accgccgtca
      721 ggatcgagtc gatcgagaac accaggtcga gcatcaggat ctgcacgatc gccgcgccga
      781 agttgctgat cgcggtgttc ctggcgtcca tcacgtcgcc cgtcggagcc gggtcgaccg
      841 agtggtggat ctccttggtc gccttccaca gcaggaacag gccgcccgtg atcaggatca
      901 gatcacgcca ggagaaggcc gtctcgaagg ccgcatggcc gtcctcgttc agcgggccct
      961 tgatgcccag gtcgaacacc ggctgggtca gggccaccag ccaggcgatg ccgaacagca
     1021 ggccgatgcg catcagcagg gcgagcgaca ggccgatgct tcgggcgcgc ggccgctgcg
     1081 cctcgggcag cttgttcgac aggatggaga tgaacacgag gttgtcgatg ccgagcacga
     1141 tctccatcac caccagggtg accagcgccg cccaggctga ggggtcggcg gcaagttgca
     1201 gcaagctgtc catggatcgg tcggcgcctt tcacgtctgg tgaaccggaa cagcgttaca
     1261 tagcccgcct cgcgcccggt tgaaccggtt ttgcacaatt cttctggcgc gcgacttgca
     1321 ctcgctccgc aggcgtcccc gtcgggcgcc caactgggac aggaacagcc atgcaggacg
     1381 ccgccaacat caggcctctg acctccctgc gattcttcgc cgcgctgtgg gtcgtgctct
     1441 tccactactg gcccaagctg gacgtcgcga tcaccccggc gctggtcggc aagggctatc
     1501 tgggcgtgga gctgttcttc gtcctgtccg gcttcatcct ctgccacgtc tacctgtcgg
     1561 ctttcggcga gggccggttc cgctacgggg ccttcctgtg gaaccgcctg gcgcgggtct
     1621 atccgctgca tctggcgacc ctcgccggca tgatcgccct gggcgtggtc gccggcctcg
     1681 cgggcgtggc catcgactcg ggaatcctcc gctgggacgc cctgccggcc aacctgatgc
     1741 tggtgcaggc ctggggcttc gcgccggcgg cggccttcaa ccacccgtcc tggtcgatct
     1801 ccgccgaatg gttcgcctac ctgagtttcc ccgccttcgg cctgctggcc tggacgatgc
     1861 gcgcgcggcc ctggctctat gccgccggga ccctggcggc gatcgccatc ctctatccgg
     1921 ccttccaggt cctgaccggc gagtccctga cccaggcgac gatcgcctgg ggcgcgctgc
     1981 gaatcgtgcc ctgcttcgcc ctgggctgcg cgatgcacag cctgtggcgc tcgggcgcgg
     2041 tgcggagcgc gcgaatggcc gccatcggcg cccttctttc aggcgccgcc ctgctcgccg
     2101 ccgccattct tggggcgccg gacatggtca ccgtggccac cttcggcggc ctgatcctga
     2161 gcctggccgg cctcgcccag cacggttcga ctttcggatc gcaggccgtc ttcgtgtatc
     2221 tcggagagat cagctattcg acctacatga tctgcgtgcc gtggaagatc atcttcgtga
     2281 atgcagccgc gcgagttctg aatctggccg atgaaaagct gccctggccc gtgtggattg
     2341 tcttcatcgt cgccctcgtt ccactgtcgg ccgcgtccta tcacctggtc gagaagccgg
     2401 cccgcgagcg catgaaactg atggccgact catggcgtgc gcggcgtgct gtcgcagcgt
     2461 gagttgaaaa gactcttttc attcgggcgt gagataggta tcccctcacc ttcagtgtcg
     2521 ggggagacct cctgaatgcg tcaacggacc aaaaccgtcc ttggttccct ggccgccgcg
     2581 gtggtgatca gccttgcgcc cacggcgaac gccatggctg acatctactg gcagtgcgtg
     2641 ccgttcgccc gactcctgtc gggcatccag atctatggcg acgccaggac ctggtggggc
     2701 caggccgtgg gcaagtacga caccggtcgt ctgccgcgga tggggtccgt tctctgcttc
     2761 aagccgaccg gccgcatgca gctgggccac gtggccgtgg tcagccggct ggtcaccgac
     2821 cgcgtaatcc agatcaccca cgccaactgg tcgcagatcg gcggcgcgcg cgggcagatc
     2881 gaaaaggacg tgaccgtcat cgacgtgtcg ccggtcggcg actggagcat ggtcaaggtc
     2941 tggtacgatc cgatccgcga cctgggcacc tcggtctatc cgacctacgg gttcatctat
     3001 cagaacggcc aggcccagca gctggcccag accgatccgc gctactggag cgcccagggc
     3061 gtggccatgg cccgcaccgc cgcgacccag acggcgtcga cggtcggacc gatcccctcg
     3121 ccgctgggca tcatcaacca ggccgccgat gcgaccgacc ggatcgccgc cctgatcgcg
     3181 gccgccaccg gccaggcgcc ggcccaggaa gacaccgccg actgaaccgg cggcggcggt
     3241 tgacgccgtc cgcgacccgc tccattcgga aggcgggtcc ggaggattca cgtcatgctc
     3301 agggtgcttc gttgcggcgg aggcgggctg cagcctgtcg aggcaggccc cgactgggtc
     3361 ctccccgagg acgtcgcctg gatcgacctg acccggcccg agcgggacga ggagctgatc
     3421 gccgagaagg cgctcggcgt cctgttgccg acccgcgagg aaatggccga gatcgaggtc
     3481 tcgtcccgtc tctatcagga ggacggggcc agcttcatga ccgccttcgt gctggtgggc
     3541 gccgatggcg acgatccggc gctcgagcct gtcaccttcg tgctggcgcg gggcaggctg
     3601 atcaccatcc gctatgtcga gccccgcgcc ttcgtcgcct acgccgccca ggcagagcgc
     3661 gacgcggact tctgctcggg cgcgcttgag gccttcctcg ggctgctcga cgccctcgtc
     3721 gaccggatcg ccgacattct tgaaaagacc tccgccgacg tcgaggtgat ctccggcgag
     3781 attttccgcc cgtcgcagcg ggcgcccttc aagctgatcc ttcggcggct gggccgcagt
     3841 cagtcgatcg ccgccaaggc gcgcagcagt ctggtcagcc tggcccggct gctgtccttc
     3901 gccgccctga cggaggccat cgccgacagc aaggagcacc gcgatcgcct gcgctcgctg
     3961 cagcgtgacg tgcagtcgct caccgaccac tcggcctatc tgacggagaa cgtcaccttc
     4021 ctgctcgatt cggcggtcgg cctgatcaac accgaccaga acgagatcat gaaggtcttc
     4081 tcggtctggg ccgtgatcct catgccgccg accctgatcg gctcggtcta cggcatgaac
     4141 ttcacacaca tgcccgagct gggctgggtc ggcggctatc cgatggccct cgggctgatg
     4201 gtgctgacca tgatcgcccc gatctggtgg ttccggcgca agggctggct ctgaaggggc
     4261 cgccggcggc ggaaagaacc gttcattaag ccggcgcgcg cgatgattcc ggaatctcgg
     4321 aaccagggtc agatgtccgc cgcgccaagc ctaatccgcg ccctcgattt cagcgccgtc
     4381 aaggcggtgg tcgaggcgca tgaccgctac ctcggcggcc ggagcggcgg caaacgcgcc
     4441 aacctgacct atgtcgacct gtcgaacatg gatctcggcg gctgcgacct ggccgacgcc
     4501 gacttcaccg gcgcccgcct ggccacgacc aacctgaacg gggccaacct caaacgggcg
     4561 aacatgttcg gctgcgacct gcgggaggcg gacctgcgcg ccgcggacct gagccaggcc
     4621 gatctgcgcg gcgcctgcct gcgcggcgcc aacctggccg gcgccaacct ggccggctgc
     4681 gatctgcgcg agggccagat cgccctgcag gggggcgccc aggcgttcag gatcctcaag
     4741 cacgagcgca aggccggcga gctggactac gccgtgcttc agggcgccaa tctggccggc
     4801 gcccagatga ccggcgccca cgccatttcc gccgacttca ccgaggccaa cctgtcgggc
     4861 gcctgcctca acgg
//
LOCUS       DAORNX010000208         4855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_12999_length_4855_cov_8.649792, whole genome shotgun sequence.
ACCESSION   DAORNX010000208 DAORNX010000000
VERSION     DAORNX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12999_length_4855_cov_8.649792"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO65_09850"
     CDS             complement(<1..216)
                     /locus_tag="PLO65_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD28590.1"
                     /translation="MTEQTPLFAVYGAPPHEVIALTADAVQVSPLVPGAARLEDLAEG
                     ALAGIALLAPPGTVERRYAMALALKALS"
     gene            complement(389..868)
                     /gene="greA"
                     /locus_tag="PLO65_09855"
     CDS             complement(389..868)
                     /gene="greA"
                     /locus_tag="PLO65_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRD28591.1"
                     /translation="MSVAFTKEEDYESTAADLPDRPISPHPNLVTPQGLALIEEALAE
                     ARAAMTSAKSGGDVSADRTAMARATRDLRYWSSRRASAQLVERGEDDGTLRFGDTVTF
                     EREDGRRQTFRIVGEDEADPARGSVSWISPLGQALLGKAVGDEAVVAGAAVEIVAIG"
     gene            1067..1633
                     /locus_tag="PLO65_09860"
     CDS             1067..1633
                     /locus_tag="PLO65_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRD28592.1"
                     /translation="MHAARPLANDLFSSLLFAALIAMNVDRQVATLVAIAFGVGHVAL
                     WLLLHKPVAPLQWASLALVLTFGAASLLFHDARFLMAKPTLIYLILTAMMLKRGWMLR
                     YMPSVAVGHGEGPMIVFGYVWAGLMALTGIANLVIAVRFPEIWPAFLAIFPTASKVAL
                     FAVQFATVRHLTIRSVRAGRAPTGAEVA"
     gene            1691..2596
                     /locus_tag="PLO65_09865"
     CDS             1691..2596
                     /locus_tag="PLO65_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28593.1"
                     /translation="MTFKPDDLMYRPGLMNGERILITGGGTGLGKVMAEACLMLGATV
                     YICGRRGNVLEETAKELMAAHGGKVVGIPCDIRVPEAIKDMIDTIWADGGALTGLVNN
                     AAGNFISRTEDLSPRGFDAIANIVFRGSFFVTLECGKRWIAEGRKASVISILTTWVWN
                     GGPFTVPSAMSKAALNVMTQSLAVEWGPKGIRFNAIAPGPFPTEGMSARLNPGQQQSA
                     YENMSGNPMNRVGEMAELANLAVYLLSKGSEYVNGQTIAIDGGQYNASGGNFSRLSEW
                     GDDQWQAVKDMIRATNDKDRAQRTV"
     gene            2593..3075
                     /locus_tag="PLO65_09870"
     CDS             2593..3075
                     /locus_tag="PLO65_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD28594.1"
                     /translation="MSHPRPRLTVRLLLVSPAGRLLLMRFEDPDNAGGGWCTIGGGVD
                     DGETLEQAAWRELREETGLTEMVLGPRVWTRELDLIVEGEPRRLFEHYFLARAPHEEV
                     VDDHWTELERRVVTAMRWWHPHEIAASDEVFFPGDLTLRLPAVLAGDIPAEPVRLNSW
                     "
     gene            complement(3079..4626)
                     /locus_tag="PLO65_09875"
     CDS             complement(3079..4626)
                     /locus_tag="PLO65_09875"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD28595.1"
                     /translation="MTSQPDPNRPDGTDFLRGGGELGALIRAHDWAATPLGPPGTWSQ
                     PLKTAVRLLLSTGHPMFLWWGPDLIQFYNDAYRRSIGPERHPGAVGQRGRDCWQEIWP
                     IIGPQIEQVMAGGEPTWHENALVPITRNGRLEEVYWTYSYGPVHDEAAPNGVGGVLVV
                     CTETTEAVLADRRRAETVTRWRRLFDQAPSFMCILGGPDHVFEFVNDAHRALFGSHDW
                     IDRPIREAFPELAGQGVYETLDAVYATGERRVFEAAPLRFRGINGGPATLRYLDFVCA
                     PIIGDDGAVVGIFWQGVDITARKDAETHLRLMVNELNHRVKNSLATVQAIAAQTLHGD
                     AVPDHIRNALTERLIALARAHDVLTDEKWVGAGLSDIAARAAAPHLSADHGDRFVIEG
                     PPVHLPPRSAIAVALALHELATNAAKYGALSVPEGRVRIDWTTAAGEAGDTHLRMTWR
                     ESGGPPVSPPRARGFGSRLIERGLATELRGRVRIDYPPSGVICTADAIIPAPDVIDWD
                     IRANLEA"
BASE COUNT          729 a         1689 c         1676 g          761 t
ORIGIN      
        1 cgagagcgcc ttcagcgcca gggccatggc gtagcgccgc tcgaccgtgc cgggcggcgc
       61 gaggagggca atgccggcca gggcgccttc ggccagatcc tcgaggcgcg cggcgcccgg
      121 aaccaggggc gagacctgca cggcgtccgc ggtcagcgcg atcacctcgt gcggcggggc
      181 gccatagacg gcgaagaggg gcgtttgttc agtcacgccg tccctatagc gatgcaacgc
      241 gctttcgcca tgccactttt ggccgggtgt tttcctgggt ccttcgacac ggcccttcgg
      301 gcctgctcag gatgacgtga aactgggcca cgccgcccct ccacgtcgtg gtgagcagcg
      361 cgaagcgcgt gtcgaaccac gcaacaggtc aaccgatcgc gacaatctcc accgccgcgc
      421 ccgccacgac cgcctcgtcg cccacggcct tacccagcag cgcctgaccc agcggggata
      481 tccacgacac actgccccgc gccggatcgg cctcgtcctc gccgacgatg cgaaaggtct
      541 gccgacggcc gtcctcgcgc tcgaatgtga cggtgtcgcc gaagcgcagg gtcccgtcgt
      601 cctcgccgcg ttcgaccaac tgggcgctgg ccctccgcga cgaccagtag cgcagatccc
      661 gcgtcgcccg cgccatggcc gtgcgatccg cgctgacgtc gccgccggac ttggcggacg
      721 tcatcgccgc ccgcgcttcg gcgagggctt cctcgataag ggcgagccct tgcggcgtga
      781 cgagattggg gtgaggcgag atcggccggt ccggcagatc cgccgccgtc gactcgtagt
      841 cctcttcctt ggtgaaggcg acgctcatgg ccccagacta gcggatgttg gccgacagcg
      901 ggcccaggat tgtccggacg gggttaaggc cattggcgaa gcgccaaacg caggccgaat
      961 ttcgcggacc gccgcgcagc cttcgcgaac agactcaccg gccgcagacc gccacgccta
     1021 gtcttgcgtg atcagatcca accggaccct tctcatgcga aacttcatgc acgccgcccg
     1081 cccgctggcg aacgacctct tctccagcct gctgttcgcg gcgctcatcg ccatgaacgt
     1141 ggaccggcag gtggcaaccc tggtggcgat cgccttcggc gtcggccacg tcgccctgtg
     1201 gctgctgctg cacaagcccg tcgcgcccct gcaatgggcc agtctggcgc tggtgctgac
     1261 cttcggcgcg gccagcctgc tgtttcatga tgcccgcttc ctgatggcca agccgaccct
     1321 gatctatctg atcctgacgg cgatgatgct gaagcggggc tggatgctgc gctacatgcc
     1381 atccgtggcc gtcggacacg gcgagggccc gatgatcgtc ttcggctatg tctgggccgg
     1441 gctgatggcg ctgaccggga tcgccaacct ggtgatcgcg gtgcggttcc ccgagatctg
     1501 gcccgccttc ctggcgatct tcccgaccgc ctcgaaggtc gcgctgttcg cggtgcagtt
     1561 cgccacggtg cgtcacctga cgatccgcag cgtccgggcg gggcgggccc cgaccggtgc
     1621 ggaggtcgcc tgaccccttg accgccctac cgtaaggcgg gcccctctgt cgcaaacagg
     1681 gagaaccgcc atgaccttca agcccgacga cctgatgtac cgccccggcc tgatgaacgg
     1741 ggagcggatc ctgatcaccg gcggcggcac ggggctgggc aaggtgatgg ccgaggcctg
     1801 cctgatgctg ggcgcgaccg tctacatctg cggccggcgc ggcaatgtgc tggaggagac
     1861 ggcgaaggaa ctgatggccg cccacggggg caaggtcgtc ggcatcccct gcgacatccg
     1921 cgtgcccgag gcgatcaagg acatgatcga tacgatctgg gctgacggcg gggcgctgac
     1981 gggcctggtc aacaacgcgg cgggcaactt catcagccgc accgaggatc tgtcgccgcg
     2041 cggcttcgac gccatcgcca acatcgtctt ccgcggctcg ttcttcgtca ccctcgaatg
     2101 cggcaagcgc tggatcgccg aaggccgcaa ggcttccgtc atttcgatcc tgaccacctg
     2161 ggtgtggaac ggcgggccgt tcacggtgcc cagcgccatg tccaaggccg cgctcaacgt
     2221 catgacccag tccctggccg tcgaatgggg ccccaagggc attcgtttca acgccatcgc
     2281 gcccgggccc ttcccgactg agggcatgag cgcgcgcctg aacccgggcc agcagcagag
     2341 cgcctacgag aacatgagcg gcaacccgat gaaccgggtc ggggagatgg ccgagctggc
     2401 caacctcgcg gtctatctgc tgtcgaaggg gtcggagtat gtgaacggcc agaccatcgc
     2461 catcgacggc ggccagtaca acgccagcgg cggcaacttc tcgcggctca gcgaatgggg
     2521 cgacgaccag tggcaggcgg tgaaggacat gatacgggcg accaacgaca aggaccgggc
     2581 gcagcgaacc gtatgagcca tccgagaccg cggctgaccg tccgactcct gctggtttcg
     2641 ccggcggggc ggctgctgtt gatgcggttc gaggatccgg acaacgccgg cgggggctgg
     2701 tgcaccatcg gcggcggggt cgacgacggc gagacgctcg agcaggccgc ctggcgcgag
     2761 ctgcgcgagg agaccggcct gaccgagatg gtgctgggtc caagggtctg gacccgcgag
     2821 ctcgacctga tcgtcgaggg cgagccgcgg cggctgttcg agcactattt cctcgcccgg
     2881 gcgcctcatg aagaggtggt cgacgaccac tggaccgagc tcgaacggcg ggtggtgacg
     2941 gccatgcgct ggtggcatcc gcacgagatc gccgccagcg acgaggtgtt ctttcccggt
     3001 gacctgacgc ttcggcttcc ggcggtcctg gccggcgata ttccggccga gccggtccgg
     3061 ctcaacagct ggtgagggtc acgcctcgag gttggcgcgg atatcccagt cgatgacgtc
     3121 cggcgccggg atgatcgcat cggcggtgca gatcacgccg gagggcggat agtcgatacg
     3181 gaccctgccc cgcagctccg tggccagccc gcgctcgatc agtcggctgc cgaagccccg
     3241 cgcccggggc ggggacaccg gcggtccgcc gctctcccgc caggtcatcc gcagatgggt
     3301 gtcgcccgcc tcgcccgccg cggtggtcca gtcgatgcgc acccgcccct ccgggaccga
     3361 caaggcgccg tacttggcgg cgttggtcgc cagttcatgc agcgccagcg ccacggcgat
     3421 cgcgcttcgc ggcggcaggt gaaccggcgg cccctcgatg acgaaccggt cgccgtgatc
     3481 ggccgacaga tggggcgccg cggcccgggc ggcgatgtcg ctcagccccg cgccgaccca
     3541 tttctcgtcg gtcagcacgt catgcgcccg cgccagggcg atcagccgtt cggtgagggc
     3601 gttgcggatg tgatcgggca cggcgtcgcc atgcagcgtc tgggccgcga tcgcctggac
     3661 cgtggccagg gagttcttca cccggtggtt cagctcgttg accatcagcc gcaggtgggt
     3721 ctcggcgtcc ttgcgggcgg tgatgtccac gccctgccag aagatgccga caacggcccc
     3781 gtcgtcgccg atgatcggcg cgcagacgaa gtctaggtag cggagcgtcg cgggacctcc
     3841 gttgatcccg cggaagcgca gcggcgcggc ctcgaagacc ctgcgctcgc cggtggcgta
     3901 gaccgcgtcg agggtttcgt agacaccctg gccggcaagc tcggggaacg cttcgcgtat
     3961 cggtctgtcg atccagtcat ggctgccgaa cagcgccctg tgggcgtcgt tgacgaactc
     4021 gaagacatgg tcgggcccgc cgagaatgca catgaagctg ggcgcctggt cgaacagccg
     4081 gcgccagcgg gtcaccgtct cggcgcgcct tcggtcggcc agcaccgcct cggtcgtttc
     4141 ggtgcagacc accagcacgc cgccgacgcc gttgggcgcg gcttcgtcgt gcaccgggcc
     4201 atagctgtag gtccagtaga cctcctcgag ccggccgttg cgggtgatcg gcaccagggc
     4261 gttctcgtgc caggtcggct cgccgccggc catgacctgt tcgatctgcg ggccgatgat
     4321 cggccagatt tcctgccagc agtcgcggcc gcgctggccc accgcgcccg gatgccgctc
     4381 cggaccgatc gatcgacgat aggcgtcgtt gtagaactgg atcaggtcgg gcccccacca
     4441 caggaacatc ggatggccgg tcgacagcag caggcggacg gcggtcttca ggggctggga
     4501 ccaggtcccg ggcggcccca gcggcgtagc ggcccagtcg tgcgcgcgga tcagggcccc
     4561 cagttcgccg ccccctctga gaaagtccgt cccgtcggga cggttcgggt ctggctgcga
     4621 agtcatagag tcccgcccca cgccgctggc cgcggctctt gacctgcaga accttagacc
     4681 ggcgcgattg ttccggaaac gccgccttgt ggcgaccgtc atggttaatc aggccttaac
     4741 cggcgcggcg ccagggtcgg gccatgcgcc gtctcgccgc ctgcctggcc ctcagcctcg
     4801 tcctcgccgc cccggcggcg gcgcagcagg gccgcatcgc cagcggccag gcctc
//
LOCUS       DAORNX010000209         4849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13027_length_4849_cov_6.426158, whole genome shotgun sequence.
ACCESSION   DAORNX010000209 DAORNX010000000
VERSION     DAORNX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13027_length_4849_cov_6.426158"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLO65_09880"
     CDS             complement(<1..751)
                     /locus_tag="PLO65_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28596.1"
                     /translation="MTKTTHTLAVLAAGTFAVAGLGAPAPALAQYRTPPAQSLGDSCR
                     DVQTLNSGYVTAVCRDTQGRYRWSSIYYPYCRSDLSNRNGVLACLGAEATVGGYVEQR
                     DSSDSSPVGAILGAIAGALLGGGDQALYAPGARYPSWGEQGYGDPRYDPRFGEQGWGY
                     GARGWVPINQRRAWLERRIARGQQQGSLTRAETTSLRRELTRLERLEMRYRGGGLSNS
                     ERADLDRRFDALAARIRAERRDEDAGWTGINQ"
     gene            complement(1035..1607)
                     /gene="rsmD"
                     /locus_tag="PLO65_09885"
     CDS             complement(1035..1607)
                     /gene="rsmD"
                     /locus_tag="PLO65_09885"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRD28597.1"
                     /translation="MRIVSGAHRGRALKTPPGDATRPTSDRARQAVFNILEHAPWAPE
                     LHGARVIDLFAGSGALGFEAISRGAGFCLFVETDDAARGAIRENIDALGLFGATRIHR
                     RDATDLGSRPAAMGEPFDLALLDPPYRRGLADKALDGLAAGGWLKPGAIAMVERASDE
                     PTPAPRGFASLDIRDYGAARVQFLRFDGPA"
     gene            complement(1613..2254)
                     /locus_tag="PLO65_09890"
     CDS             complement(1613..2254)
                     /locus_tag="PLO65_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD28598.1"
                     /translation="MGWLRWLLLLLVARPIARFLTGADVSGREHLPLKGPAIVAANHN
                     SHMDTLLLLSIFPSRAVMRVRPAAASDYFLRDPVIGWFSRNLIGIVPIARGRAGTGED
                     VLAPAREALAAGDIIVVFPEGTRGDASDEMAALKSGVARLAEAFPEAPVTPVWIQGAG
                     RVLPKGEVIPVPLNCAVLIGEPVRWQGGRTAFMEDLRAALLELKEQAPPLRWS"
     gene            complement(2254..3225)
                     /locus_tag="PLO65_09895"
     CDS             complement(2254..3225)
                     /locus_tag="PLO65_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRD28599.1"
                     /translation="MNVIDRILAFHAAQPLVLLWGLGGIIGLLVLGSVVAAVLTLARP
                     QKDYRELRQRMSSWWVMIALIAAALLLGWQATLGLFAVVSFIALREFLSLAPTRREDR
                     LIILACYLAIPVSYAFVAADIYGIYLVVIPVYAFLTLPWLMAVIGQTKGYLSSVALFH
                     WGLMTCVYNIGYAAFLMRTPAEEAPAGAAGLVFFLLVATEFNDVAQYVWGKLFGRHKI
                     IPKVSPNKTWEGFLGGWATTAALIWFVGPLFMPLGGVGLATMALVVPVAGFAGDVTMS
                     AIKRDIGVKDTSHLIPGHGGILDRIDSLTFTAPLYFHLLAWFSLEKF"
     gene            3432..3818
                     /locus_tag="PLO65_09900"
     CDS             3432..3818
                     /locus_tag="PLO65_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28600.1"
                     /translation="MQRLMTSSLVHVAFGFLLMGGWAYFANRAHGWQGAWAPALAQGV
                     VSGALTGGLKKTLEALDGRIAGPLAFVIPPSVTAGSIIALLVLVHRLIGTPELLATIA
                     FPWSVSTLYAIVYNAGLVRARRRSAT"
     gene            3815..4489
                     /locus_tag="PLO65_09905"
     CDS             3815..4489
                     /locus_tag="PLO65_09905"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD28601.1"
                     /translation="MSDSLENRRPLKTRSKAWAGALAGAMGEARVSPDLISLSSVIFA
                     LLGGALLLAAGLEESRVLRGLMLVMAAVMVQLRLLANMLDGMVAVEHGLGSSAGPIWN
                     ELPDRIADALFLVGAGYAAWGLGVTAGVWLGWTAALLAVLTAYVRELGRGLGFPADFA
                     GPMAKPHRMFALTVTCLIAALEPLWGGRGQVMMIGLTIIVLGTVVTLVSRTRTLARRL
                     EAQRAK"
BASE COUNT          681 a         1688 c         1776 g          704 t
ORIGIN      
        1 gctggttgat gccggtccag ccggcgtcct cgtcgcgacg ctcggcgcgg atacgggccg
       61 ccagggcgtc aaaccgccgg tcgaggtcgg cgcgttcgct gttcgacaag ccgccgccgc
      121 ggtagcgcat ttcaagccgc tcgaggcggg tcagttcgcg gcgcaggctg gtggtctcgg
      181 cccgggtcag agatccctgc tgctggccgc gcgcgatgcg gcgctccagc caggcgcgcc
      241 gctggttgat cggcacccag ccccgggcgc cgtagcccca gccctgttcg ccgaagcgcg
      301 ggtcgtagcg cgggtcgccg tagccctgtt cgccccacga cgggtagcgt gcgcccgggg
      361 cgtagagtgc ctggtcaccg ccgcccagaa gggcgccggc gatggcgccg agaatcgccc
      421 ccacgggcga gctgtccgac gagtcgcgtt gttcgacgta gccgccgacc gtggcttcag
      481 cgccgaggca ggcgagcacg ccgttgcggt tggacaggtc gctgcgacag taggggtaat
      541 agatcgagct ccagcggtag cggccctggg tgtcgcggca gacggcggtc acatagccgc
      601 tgttcaacgt ctgcacgtcg cggcaggagt cgcccaggct ctgggctggc ggcgtccggt
      661 actgggccag ggccggggcc ggagcaccca ggcccgcgac ggcgaacgtc ccggcggcga
      721 gcacagccag ggtgtgggtc gtcttggtca tcgatctctc ccgagaaggc gagcgccggc
      781 gcgcggcgac gcgcgcggcg acgtggcatg gcgtggaact gtctgggaaa cgacccgccg
      841 gccgttaagt tgcgtgcaat caagaccggg cccggagcgg gcgcccgggt cactgtactg
      901 acgcggggcg cgcggcttcc ggttccgtcc gccggccggg ggcgccgacg cgccgttcga
      961 cctgcgccgt ctgtcagggc ggacctggcc ggaaggacgg gttcctgcct gcactgtgca
     1021 ggcggacggc ctggtcaggc agggccgtcg aaccgcagga actgcacccg cgccgcgccg
     1081 tagtcgcgga tgtccaggga agcgaaaccg cggggcgcgg gagtcggctc gtcgctcgcc
     1141 cgctcgacca tcgcgatcgc gcccggcttc agccagccgc cggcggccag gccgtccagg
     1201 gccttgtccg ccagccccct gcgatagggc gggtcgagga gggccaggtc gaacggctcg
     1261 cccatcgccg ccggccgcga tccgaggtcg gtcgcgtcgc gacggtggat gcgtgtggcg
     1321 ccgaacaggc ccagcgcgtc gatattctcg cggatcgcgc cgcgcgcggc gtcgtcggtc
     1381 tcgacgaaca gacagaagcc ggccccgcgc gagatggcct cgaagcccag cgcgcctgac
     1441 cccgcgaaca ggtcgatgac ccgggcgccg tggagctccg gggcccaggg cgcatgctcg
     1501 aggatgttga acaccgcctg ccgcgcccgg tcgctggtcg ggcgggtggc gtcaccgggc
     1561 ggggtcttca gggcgcgccc gcgatgggcg ccggatacga tgcgcaaggc cttcagctcc
     1621 agcgaagcgg cggggcctgc tccttcagct caaggagggc cgcgcggagg tcttccataa
     1681 acgcggttcg tcccccctgc caacggaccg gctcgccgat cagcaccgcg caattcagcg
     1741 gcacggggat cacctcgccc ttgggcagca cgcggccggc gccctggatc cacaccggcg
     1801 tgacgggcgc ctccggaaac gcctccgcca gccgggccac gccgctcttg agcgcggcca
     1861 tttcgtcgct ggcgtcgccg cgggtgcctt cgggaaacac cacgatgatg tcgccggccg
     1921 cgagcgcctc gcgggccggg gccagcacgt cctcgccggt cccggccctg ccccgggcga
     1981 tcggcacgat gccgatcagg ttgcgcgaga accagccgat caccgggtcg cgcaggaagt
     2041 agtccgaggc cgccgccggc cgcacgcgca tcaccgcgcg gctggggaag atgctcagca
     2101 gcagcagggt gtccatgtgg ctgttgtggt tggccgcgac gatggccggg cccttcagcg
     2161 gcaggtgctc gcgcccgctc acatcggcgc cggtcaggaa ccgcgcgatc ggccgggcga
     2221 ccagcagcag caacagccag cgcagccagc ccatcagaac ttctccagcg agaaccaggc
     2281 cagcaggtgg aaatacagcg gcgcggtgaa ggtcaggctg tcgatccggt cgaggatgcc
     2341 gccgtggccg gggatcaggt gcgaggtgtc cttgaccccg atatcgcgct tgatcgcgct
     2401 cattgtcaca tcgcccgcga aacccgccac gggcaccaca agagccatgg tcgccagtcc
     2461 gaccccgccc agcggcatga acagcggccc gacgaaccag atcagcgccg ccgtcgtcgc
     2521 ccagccgccg aggaagccct cccaggtctt gttgggactg accttcggaa tgatcttgtg
     2581 gcgcccgaac agcttgcccc agacgtactg cgccacgtcg ttgaactcgg tggcgaccag
     2641 caggaagaac accagacccg ccgcgccggc cggcgcttcc tccgccggcg tgcgcatcag
     2701 gaaggccgcg tagccgatgt tgtagacgca ggtcatcagc ccccagtgga acagggcgac
     2761 gctcgacaga tagcctttcg tctggccgat caccgccatc agccagggca gggtcagaaa
     2821 ggcgtagacc gggatgacca ccaggtagat gccgtagatg tcggccgcca cgaaggcgta
     2881 gctgaccggg atcgccaggt agcaggcgag gatgatcagc cggtcctcgc gccgggtcgg
     2941 agccagcgac aggaattcgc gcagggcgat gaagctgacc accgcgaaca ggcccagcgt
     3001 cgcctgccag cccagcagca gggccgcggc gatcagggcg atcatcaccc accagctcga
     3061 catgcgctgg cgcagctcgc ggtagtcctt ctgcggccgg gcgagcgtca ggacggcggc
     3121 gacgacgctg cccagcacca gcaggccgat gatcccgccc aggccccaca gcaggaccag
     3181 cggctgcgcg gcgtggaagg ccagaatccg gtcgatgacg ttcattcaca gatcctggcc
     3241 gggcgaggtc gccgacggaa gccttgcgcg ggcatccctg catggttcga cacgcgctct
     3301 tcgagcgctg ctcgccatga cgtacacaat cgcacgtcat cctgagcagc cgcgaagcgg
     3361 cgtgtcgaag gacgcaggac agcactttca gggaccttgc cgcgcgcagc gatccggtca
     3421 aggctccagg aatgcagagg ttgatgacgt cgagcctggt ccatgtcgcg ttcggcttcc
     3481 tgctgatggg cggctgggcc tacttcgcca atcgcgccca tggctggcag ggggcctggg
     3541 cgccggccct ggcccagggg gtcgtctccg gcgccttgac cggagggctg aagaagacgc
     3601 tggaggccct cgacgggcgg atcgcggggc cgctggcgtt cgtgattccg ccgtccgtga
     3661 cggcaggatc gatcatcgcc ctgctggtgc tggtccaccg cctgatcggc acgcccgagc
     3721 tgctcgcgac catcgccttt ccgtggagcg tctcgacgct ctacgccatc gtctacaacg
     3781 ccggcctggt cagggcccgc cgccggagcg ccacatgagc gacagtctcg agaaccggcg
     3841 gccgctgaag acccgctcga aggcctgggc cggggcgctg gcaggcgcga tgggcgaggc
     3901 cagggtcagt ccggacctga tctccctgtc ttcggtgatc ttcgcgctgt tgggcggggc
     3961 gttgctgctg gccgccgggc tggaggagag tcgcgtcctg cgcggcctga tgctggtcat
     4021 ggcggcggtc atggtgcagc tgcggctgct ggccaacatg ctcgacggca tggtcgcggt
     4081 ggagcacggg ctggggtcga gcgcggggcc gatctggaac gagctgcccg accggatcgc
     4141 cgacgccctg ttcctcgtcg gcgccggcta cgcggcctgg ggcctgggag tgacggccgg
     4201 cgtctggctg ggctggacgg ccgccctgct ggccgtgctg accgcctatg tgcgcgagct
     4261 cggccgtggc ctgggctttc cggccgactt cgccggcccg atggccaagc cgcatcggat
     4321 gttcgccctg acggtgacct gcctgatcgc ggcgctcgag ccgctgtggg gcgggcgggg
     4381 gcaggtgatg atgatcgggc tgacgattat cgtgctcggg acggtcgtca cgctggtcag
     4441 ccgcacccgg accctggcgc ggcggctgga agcgcagcgg gcgaaatagc acggggacat
     4501 gcacttcagc gcgtgtcccc tcggggcggc tagcgcttct ttcccggccc gccggcgcga
     4561 gcccctttgg gccctccggt cggacggggg ccacgcgggc ccgttggctt cggggcgccc
     4621 gacggcctgg cgaaggatcg cggattcgcc gccccctcgg gtcgcgcccc ctttggtccc
     4681 tccgaccagt gcgtcttcgc cttgccggcc ggggcggcct tcttcggggt gcgcaggccg
     4741 ccgggcttct tcggcctggc cttcgcccaa cccggcttct tcggctggcg ctcgcccttc
     4801 cgttcgccgg tttcctggcc ggggcgcggc acggtgcggt gggcccggg
//
LOCUS       DAORNX010000210         4846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13041_length_4846_cov_8.307243, whole genome shotgun sequence.
ACCESSION   DAORNX010000210 DAORNX010000000
VERSION     DAORNX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13041_length_4846_cov_8.307243"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="yihA"
                     /locus_tag="PLO65_09910"
     CDS             <1..504
                     /gene="yihA"
                     /locus_tag="PLO65_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRD28602.1"
                     /translation="RSNVGKSSLINALVGQKYLARASNEPGRTREINFFLLDGRLRLV
                     DLPGYGFARVSRTSKDRFQNLGRQYLRGRPNLKRVYLLIDARHGLKEPDTEALDALDT
                     AAVSYQIVLTKADKIKPAEVEAVVARTLTQIAKRPAAFPRVLATSSEKGAGIPILRAE
                     IKATCGI"
     gene            complement(610..1881)
                     /locus_tag="PLO65_09915"
     CDS             complement(610..1881)
                     /locus_tag="PLO65_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD28603.1"
                     /translation="MRRVLAAFTLALAMSSPLAAQAKTVAVTAARLLDVATGKYVEQP
                     LVVITDGRITAVGTQATMTVPADAEKIDLPGQTLLPGLIDMHVHLDSSPKYGGYAGLA
                     FTDSFWAVVGTANAAATLNAGFTSVRNVGADRYNDVALMQAIDEGIVPGPRITPAGYA
                     LGATGGHCDSTYFPPSVDLTSPGVFDGPDEARKLVRKMKKYGAKVIKICATGGVFSND
                     AVGIQQLTVEEIKAIVDEAHMDGMKVAAHAHGPEGIRAAILGGVDTIEHASLVDDEGI
                     KLALKYGAAFGMDIYNTDYTQAEGRANGVRESELKKDHDIGEAQRQNFCKAVKAGVTQ
                     IYSTDAGIYPHGLNARQFSVMVRYCATPLQAIQAATINAAAALGQAGDVGQVAVGRWG
                     DLIAVQGDPLADVSVLEHVTFVMKGGAVVRR"
     gene            2026..3114
                     /locus_tag="PLO65_09920"
     CDS             2026..3114
                     /locus_tag="PLO65_09920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28604.1"
                     /translation="MRIDVVRPWELTACAVERWAALQAADPALDSPFLSPYWPRSVAA
                     AQRQDGGERGLRVAVVGGMADPKGFIAARVGAFTAIPAGAPMCDYQGLVAEPGLTLDP
                     RRIAEAFGVGRFDFSHMLEDQALFAPLARGREDSFVIDVSDGYAAYEAERRGAGVSVL
                     KDTDKKRRKVERELGPVTFAAFSRSRSDFEQLVVWKRGQWRATGQTDLFAAGWPLHLV
                     RGLFASRDPDYGGILFTLHIGDQLAAAQLDLRGGRNIHSWIIGHDPAFERYSPGMLLF
                     QDILRWMDQVPYSRLDLGAGDYRFKRELANRRIGVTHGFIGAPSAAALMRETAYAVRR
                     AAESLPLGRVSQLPGKAMRRLDLIRGLR"
     gene            3145..4347
                     /locus_tag="PLO65_09925"
     CDS             3145..4347
                     /locus_tag="PLO65_09925"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRD28605.1"
                     /translation="MTLTLQDIKAAAGRLAGHVERTPCRYSRTLSEITGAQVWIKFEN
                     LQFTAAYKERGALNKLLLLSEEERARGVIAASAGNHAQGLAYHGARLGVPVTIVMPKG
                     TPFVKVEHTRQHGANVVIFGDTYDEAAAHAQQLREERGLTFVHPFDDYDVMAGQGTIA
                     LEMLEDVPDLEILPVPIGGGGLISGVATAAKAINPNVRVVGCEPAMFPSFTARMRGLN
                     AEAGGQTIAEGIAVKEVGTRTYAIARPLIDDVLLLEEPYIERAIALYCNVEKTIAEGA
                     GAASLAALLAYPERFRGKKCGLILCGGNIDTRLLASVLTRELVRAQRLVSLRIIGDDR
                     PGLLATVSRVIGEMGGNIIEVAHNRLALDVPAKGAEFDIMIETRDAQHTQEIMDALRE
                     SGYPPRAV"
     gene            4358..>4846
                     /locus_tag="PLO65_09930"
     CDS             4358..>4846
                     /locus_tag="PLO65_09930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRD28606.1"
                     /translation="MPKRALIAAAVACLALAGCARKEADISLIQANAAQSAAFLEKTA
                     KEPGVQKLPSGVLYKVVSSGPADGVSPRREDEVKVHYEGKLVSGDVFDSSFQRGAPAV
                     MRLGGLIPAWIEALQKMKPGDEWILYVPPALGYGEQGAGPIPPNSVLIFRIQLIGVLP
                     AAG"
BASE COUNT          724 a         1727 c         1665 g          730 t
ORIGIN      
        1 cgctccaacg tcggcaagtc gagcctgatc aacgccctgg tcggccagaa gtacctggcc
       61 cgggcgtcga acgagccggg ccggacgcgg gagatcaact tcttcctgct cgacgggcgg
      121 ctgcggctgg tcgacctgcc cggctacggc ttcgcccgcg tctcgcgcac ctcgaaggac
      181 aggttccaga acctcggccg ccagtacctg cgcggccggc cgaacctcaa gcgggtctat
      241 ctgctgatcg acgcgcgtca cggcctcaag gaaccggaca ccgaggcgct ggacgcgctc
      301 gacacggcgg ctgtgtccta ccagatcgtc ctgaccaagg ccgacaagat caagccggcc
      361 gaggtcgagg ccgtggtcgc gcgcaccctg acgcagatcg ccaaacgccc ggcggccttc
      421 ccccgggtgc tggccacgtc ctcggagaag ggtgcgggca tcccgatcct gcgcgccgag
      481 ataaaggcga cctgcgggat ctgattgcgt ccttcgacac ggccgctacg cggcctgctc
      541 aggatgacgt gatctgttcc acgtcatggc gagcagcgcc gaaggcgcgt gtcgaaccac
      601 gcaccgtcgc taccgccgca ccaccgcgcc gcccttcatc acgaaggtca cgtgctcgag
      661 cacgctcacg tcggccagcg ggtcgccctg gacggcgatc aggtcgcccc agcggccgac
      721 cgccacctgg cccacgtcgc cggcctggcc cagcgcggcc gcggcgttga tcgtcgcggc
      781 ctggatggcc tgcagcgggg tggcgcagta gcgcaccatc accgagaact gccgggcgtt
      841 caggccatgc ggatagatgc cggcgtcggt cgagtagatc tgcgtcaccc cggccttcac
      901 ggccttgcag aagttctgcc gctgggcctc gccgatgtcg tggtccttct tgagctcgct
      961 ctcgcgcacg ccgttggccc ggccctcggc ctgggtgtag tcggtgttgt agatgtccat
     1021 gccgaaggcg gcgccgtact tcagcgccag cttgatgccc tcgtcatcga cgaggctggc
     1081 gtgttcgatg gtgtcgaccc cgccgagaat cgcggcgcgg atgccttcgg ggccgtgggc
     1141 gtgagcggcg accttcatgc cgtccatgtg cgcctcgtcg acgatcgcct tgatctcctc
     1201 gacggtcagc tgctggatgc ccacggcgtc attggagaac accccgcccg tggcgcagat
     1261 cttgatcacc ttcgccccgt acttcttcat cttgcggacc agcttgcgcg cctcgtcggg
     1321 gccgtcgaag acgcccggcg aggtcaggtc caccgagggc gggaagtagg tcgaatcgca
     1381 atgcccgccc gtggccccaa gggcgtagcc ggccggggtg atgcgcgggc cggggacgat
     1441 cccctcgtcg atcgcctgca tcagcgccac gtcgttgtag cggtcggccc cgacgttgcg
     1501 cacggaggta aagccggcgt tcagcgtcgc ggcggcgttg gccgtgccga cgacggccca
     1561 gaagctgtca gtgaaggcca ggccggcgta gccgccgtac ttcggcgagg agtcgaggtg
     1621 gacgtgcatg tcgatcaggc cgggcagcag ggtctggccg ggcaggtcga tcttttccgc
     1681 atcggcgggg acggtcatcg tcgcctgggt cccgacagcg gtgatgcggc cgtcggtgat
     1741 caccaccagc ggctgttcga cgtattttcc ggtggccaca tcgagcaggc gcgcggcggt
     1801 cacggccacg gtcttggcct gggccgccag cggcgaggac atggcgagag ccagggtgaa
     1861 ggcggccagg acacggcgca tgatgtgagt tccccgagga tgaccggtcc gtcttagcct
     1921 cgcttcggcc cgtctcgcca gtgtgaagtc ggcatcgacc taaaccctcc tgcaagggtt
     1981 ggcggctaga gtccgctgcg gcccagacag gcggggagtg taaaggtgcg tatcgacgtc
     2041 gtcagaccct gggagctgac cgcctgcgcc gtcgagcgat gggcggccct gcaggcggcc
     2101 gacccggcgc tcgacagtcc cttcctgtcg ccctactggc cccggtcggt ggcggccgcc
     2161 cagcgccagg acggcggcga gcgcggcctg cgcgtcgccg ttgtcggggg catggcggat
     2221 ccgaagggat tcatcgcggc ccgcgtcggg gccttcaccg ccatccccgc cggcgcgccg
     2281 atgtgcgact accagggcct ggtggccgag cccggcctga ccctcgaccc gcgccggatc
     2341 gccgaggcct tcggcgtcgg ccggttcgat ttcagccaca tgctggagga ccaggccctg
     2401 ttcgcgccac tggcgcgggg tcgcgaggac tccttcgtca tcgacgtctc cgacggctat
     2461 gccgcctacg aggccgagcg ccgcggcgcc ggcgtctcgg tcctcaagga caccgacaag
     2521 aagcgccgga aggtcgagcg cgaactgggc ccggtgacct tcgccgcctt ctcgcgctca
     2581 cggtccgatt tcgagcagct cgtcgtctgg aagcggggac agtggcgcgc cacgggccag
     2641 accgacctgt tcgccgccgg ctggccgctg catcttgtgc gcggcctgtt cgccagccgc
     2701 gacccggact acggcggcat cctgttcacc ctgcacatcg gcgaccagct ggccgccgcc
     2761 cagctcgacc tgcgcggcgg gcgcaacatc cacagctgga tcatcggcca cgatcccgcc
     2821 ttcgaacgct attcgcccgg catgctgctg ttccaggaca tcctgcgctg gatggaccag
     2881 gtcccctaca gccgcctcga cctgggggcc ggcgactacc gcttcaagcg cgagctggcc
     2941 aaccggcgga tcggcgtcac ccacggcttc atcggcgccc cgtcggcggc ggccctgatg
     3001 cgcgagacgg cctacgccgt gcggcgggcg gccgagtccc tgccgctggg ccgggtgtcg
     3061 caattgccag gaaaggccat gcggcggctt gacctcatac ggggtttgcg gtgaaggctg
     3121 gcgcccccgc ccgctgaaat ccccatgacc ctcaccctgc aagacatcaa ggccgccgcc
     3181 ggccgcctgg ccggccacgt cgaacgcacg ccctgccgct actcgcgcac cctgtcggag
     3241 atcaccggcg cccaggtgtg gatcaagttc gagaacctgc agttcacggc cgcctacaag
     3301 gagcgcggcg cgctcaacaa gctgctgctg ctcagcgagg aggagcgcgc ccgcggcgtg
     3361 atcgccgcca gcgccggcaa ccacgcccag ggcctggcct atcacggcgc ccgtctcggg
     3421 gtgccggtga ccatcgtcat gcccaagggc acgccgttcg tgaaggtcga gcacacccgc
     3481 cagcacggcg ccaacgtggt catcttcggc gacacctacg acgaggcggc ggcccatgcg
     3541 cagcagctgc gcgaggagcg cggcctgacc ttcgtccacc cgttcgacga ctacgacgtg
     3601 atggccggcc aggggaccat cgccctggag atgctcgagg acgtgcccga cctggaaatc
     3661 ctgccggtgc cgatcggcgg cggcggtctg atcagcggcg tggccaccgc ggccaaggcg
     3721 atcaatccca acgtccgggt cgtcggctgc gagccggcca tgttccccag cttcaccgcc
     3781 cgcatgcgcg gcctgaacgc cgaggccggc ggccagacga tcgccgaggg catcgcggtg
     3841 aaggaggtcg ggaccaggac ctacgccatc gcccggccgc tgatcgatga cgttttgctg
     3901 ctcgaggagc cctacatcga gcgggcgatc gccctctact gcaatgtcga gaagaccatc
     3961 gccgagggcg ccggcgccgc ctccctggcc gccttgctgg cctatcccga gcggttccgg
     4021 ggcaagaaat gcggcctgat cctctgcggc ggcaatatcg acacccgcct gctggccagc
     4081 gtgctgaccc gcgagctggt gcgggcccag cggctggtct ccctgcgcat catcggcgac
     4141 gaccgcccgg gcctgctggc cacggtcagc cgggtgatcg gcgagatggg cggcaacatc
     4201 atcgaggtgg cccacaatcg cctggccctg gacgtgccgg ccaagggggc ggagttcgac
     4261 atcatgatcg agacccgcga cgcccagcac acccaggaaa tcatggacgc cctgcgcgag
     4321 agcggttacc cgccgcgcgc ggtctaggag acagacaatg ccgaaacgcg ccctgatcgc
     4381 ggccgccgtc gcctgcctgg ccctcgccgg ctgcgcgaga aaggaagccg acatctcgct
     4441 gatccaggcc aacgcggccc agtcggcggc cttccttgag aagacggcca aggagccggg
     4501 cgtgcagaag ctgccgtccg gcgtgctcta caaggtggtc agctcgggcc cggccgacgg
     4561 cgtctcgccg cgccgcgagg acgaggtcaa ggtccactac gagggcaagc tggtctcggg
     4621 cgacgtcttc gacagctcgt tccagcgcgg cgccccggcc gtgatgcggc tgggcggcct
     4681 gatcccggcc tggatcgagg cgctgcagaa gatgaagccc ggcgacgaat ggatcctcta
     4741 cgtcccgccg gcgctcggct acggcgagca gggcgcgggc ccgatcccgc ccaacagcgt
     4801 gctgatcttc cgcatccagc tgatcggcgt cctgccggcg gcgggc
//
LOCUS       DAORNX010000211         4827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13110_length_4827_cov_7.615046, whole genome shotgun sequence.
ACCESSION   DAORNX010000211 DAORNX010000000
VERSION     DAORNX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13110_length_4827_cov_7.615046"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLO65_09935"
     CDS             complement(<1..862)
                     /locus_tag="PLO65_09935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRD28607.1"
                     /translation="MPAQESSQQHDRVAKVLARAGVASRREVERLISEGRVAINGETL
                     TTPAVKVEPGDIVTVDGEVVGGAEATRLFRYHKPVGLLTSHNDPAGRPTVFETLPEGL
                     PRVISIGRLDINSEGLLLLTNDGALSRALELPSSGWVRRYRARAFGYTTQEKLDKLKD
                     GITVEGVKYGAIDAHLDKATGDRDGARNVWITVTLTEGKNREVRRVLEAIGLKVNRLL
                     RLSYGPFSLGTLAAGEVEEVGPRVIRELLADFVPAANMPTGNAVQFKPALSAGRRSGV
                     EDGVGGAAPAA"
     gene            973..1413
                     /locus_tag="PLO65_09940"
     CDS             973..1413
                     /locus_tag="PLO65_09940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRD28608.1"
                     /translation="MRMALDEARAAAARGETPVGAVILDPATGAVLARAGNGPIGAHD
                     PTAHAEIAAIRQAAAALGNYRLTGLTLVVTLEPCAMCAGAISHARLGRVVFGAEDPKG
                     GAVVHGPRFFDQPTCHWRPQVTGGVLGEESAALLRDFFRTRRGK"
     gene            1466..1921
                     /locus_tag="PLO65_09945"
     CDS             1466..1921
                     /locus_tag="PLO65_09945"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRD28609.1"
                     /translation="MKHPLLALLLLSATPAFAGETAAVADLAWMAGDWAQESDGGTVR
                     ETWLPPLDGAMAGVTQTLRPGRAPVTEFATITTGPAGVTFTAYVGGQPATPFVLRSGK
                     PGEAVFENPDHDFPQRVIYRRCDADLCARIEGTVGGQVRGMDWRYRRLP"
     gene            complement(1925..2257)
                     /locus_tag="PLO65_09950"
     CDS             complement(1925..2257)
                     /locus_tag="PLO65_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28610.1"
                     /translation="MTDPLAIHVDPDDLIALAMELRDEPVSLVVLDGDEASTALLAAL
                     NPADLRSVLAGRLMVEGLDGLLTVITHDGVRLVEPQADAPPPPRLAFDGMGVGRPESG
                     AEPLRLAG"
     gene            complement(2525..3121)
                     /locus_tag="PLO65_09955"
     CDS             complement(2525..3121)
                     /locus_tag="PLO65_09955"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD28611.1"
                     /translation="MNLKAKVLKGRHVRLEPITAALRDELRGAIDCDPASWEIMSTNG
                     CGEGFADWWGALQGETERGERIGYAIRRLSDKRVVGTSSFLNIRRIHKGVEIGATFLH
                     PDVRSGPVNPESKRLMLAQAFDAGAIRVEFMIDERNARSEAAVLKLGATKEGTLRNHK
                     ITWTGHVRDTAVLSICDFDWPAVRDRLDFRLRESFSPA"
     gene            3132..3746
                     /locus_tag="PLO65_09960"
     CDS             3132..3746
                     /locus_tag="PLO65_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28612.1"
                     /translation="MPKAATVGNSLRKINVAPLKIYVLYKKNDVPHGAATMPYDTSDK
                     SLRRQATFLQAATVMIVAATVLNYLRTGEVIPFLFALFGTGLTQVEIDGLLKGLALAL
                     PGFFYAGAVLVAGGIFGRIATGELFSARNSAALGAVGGWLSMGAIVAMIVTPVVVDWL
                     DLGALRLRTEPTDMLIAVIGGLIQILGFVHTRATALRSELDQII"
     gene            3759..4277
                     /locus_tag="PLO65_09965"
     CDS             3759..4277
                     /locus_tag="PLO65_09965"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRD28613.1"
                     /translation="MSNAKLADQAKTFEVIAWIAVVVLSLQFLVFLNLLTLIGPIADA
                     IRLGDTTGLRDLFQPVLYRLVDVLPALIYLGGVWAAARIFGRVAKGELFSRANSAGLA
                     EVGSSLLWGAAASAVIVPWIRSWIAGDYGFSGIHLEIETWVIAVIGGAILVLGKMMAQ
                     AGRLQSELSEII"
     gene            4281..4517
                     /locus_tag="PLO65_09970"
     CDS             4281..4517
                     /locus_tag="PLO65_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD28614.1"
                     /translation="MPVIVTLDIMLARRKMKARDLAQRIGLSETQLSLLRTGKVRGMR
                     FDTLSKLCAVLECTPGELLDYRYDEGDLAVADPE"
     gene            4619..>4827
                     /locus_tag="PLO65_09975"
     CDS             4619..>4827
                     /locus_tag="PLO65_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28615.1"
                     /translation="MDFDISPKQRQFLDRVVAFMDEHIAPAVPRYNEEMNVIGDARWK
                     VVQVVEELKEKAKAAGLWNFFMPPH"
BASE COUNT          714 a         1666 c         1652 g          795 t
ORIGIN      
        1 gggccgccgg agccgcccca ccgacgccgt cctcgacccc gctgcgacgg ccggccgaca
       61 gggccggctt gaactgcacg gcgttgccgg tcggcatgtt ggccgccgga acgaagtcgg
      121 ccagcagttc gcggatcacc cgcggcccga cctcctcgac ctcgccggcc gccagggttc
      181 ccagcgagaa cgggccatag ctcagccgca gcagccggtt gaccttgagc ccgatggcct
      241 cgagcacgcg gcgcacctcg cggttcttgc cctcggtcag ggtgaccgtg atccagacat
      301 tgcgcgcgcc gtcgcgatcg ccggtcgcct tgtcgagatg ggcgtcgatg gcgccgtatt
      361 tcacgccttc gacggtgatg ccgtccttca gcttgtcgag cttctcctgg gtcgtgtagc
      421 cgaacgcccg ggcgcggtag cgccggaccc agccgctcga cggcagctcc agcgcccgcg
      481 acagggcccc gtcgttggtc aacagcagca ggccttcgga attgatgtcc agccggccga
      541 tcgagatgac ccgcggcagc ccctcgggca gggtctcgaa cacggtcggc cggcccgccg
      601 ggtcgttgtg gctggtcagc agcccgaccg gcttgtgata gcggaacagc cgggtggcct
      661 cggccccgcc gaccacctcg ccgtcgaccg tgacgatgtc gcccggctcg accttgaccg
      721 ccggggtggt cagggtctcg ccgttgatcg ccacccggcc ctcggagatc agccgttcga
      781 cttcgcgccg cgaggcgacg ccggcccggg ccagcacctt ggccacgcga tcgtgctgtt
      841 gggaagattc ctgtgcaggc atgcgcgccg ctccgttaga aggacctgtg tcgcctcccg
      901 gcgatacctg ccccttctta agcacggatc gacggttccc gccacgtgag cgtccccagc
      961 gacatcgacg ccatgcggat ggccctggac gaggcgcgcg ccgcggccgc ccgcggcgag
     1021 actccggtcg gggcggtgat cctcgatccg gccacgggcg cggtgctggc gagggccggc
     1081 aacggaccga tcggggccca tgatccgacc gcccacgccg agatcgccgc catccgccag
     1141 gccgccgccg cgctcggcaa ctatcggctg accggcctga cactggtggt caccctggag
     1201 ccctgcgcga tgtgtgcggg ggcgatcagc cacgcccgtc tgggtcgggt ggtgttcggc
     1261 gccgaggatc ccaagggcgg ggccgtggtc catggcccgc ggttcttcga ccagccgacc
     1321 tgtcactggc ggccgcaggt gaccggcggc gtgctgggcg aggagagcgc cgcgctgctg
     1381 cgggacttct ttcggacgcg gcgagggaag tgacgacgcg ccggtcctga ggcaggctgg
     1441 cggtcccgga cccggagtcc ccgtcatgaa acatcctctt ctcgccctgc ttctgctgtc
     1501 cgccacgcca gcgttcgccg gagagacggc cgcggtcgcg gacctggcct ggatggccgg
     1561 cgactgggcg caggaatccg acggcgggac ggtccgcgag acatggctgc cgccgcttga
     1621 cggggccatg gccggcgtga cccagaccct tcgccctggc cgcgcgccgg tgacggagtt
     1681 cgccaccatc acaaccggtc cggccggcgt gacctttacc gcctatgtcg gcggccagcc
     1741 ggcgacgcca ttcgtgctcc ggtccggcaa gccgggcgag gcggtgttcg agaacccgga
     1801 ccacgacttc ccgcagcggg tcatctatcg ccggtgcgac gccgacctgt gcgcgcgcat
     1861 cgaagggacc gttggtggtc aggtccgggg gatggactgg cgctatcgac gcctgccctg
     1921 aggttcagcc ggcaaggcgc agcggttcgg ctccggattc cggccgtccg acgcccattc
     1981 cgtcgaaagc cagccgcggc ggaggcggtg cgtccgcctg aggctccacg agccgaacgc
     2041 cgtcgtgcgt gatcaccgtc agcaaaccgt ccagcccttc gaccatcagc cgcccggcga
     2101 gcaccgaccg cagatcggcc gggttcagcg ccgccagcaa cgccgtcgag gcctcgtcgc
     2161 cgtcgagcac gacgagcgac accggctcgt cccgcaattc catcgccagg gcgatcaggt
     2221 cgtccggatc gacatggatg gccagcggat cggtcaacag gaaggcgccg ggggccacct
     2281 cgccaaaccg ccgggggcgg gaccatcggc cgtacaggta ggcggcgacg acgcgcctca
     2341 ggccgggcgg cgggctgtcg gcgccgaccg gcgcgcggtc gggatcggcg aggatgcgaa
     2401 ggcaggcgag tgcggtcatg gccgcatctt cccgcgcagg ttgttaccag aattccgccg
     2461 gcccgcgtgt caagttgacg cacaaggccc ggttgcggcg acgggtcgca ggcggcgacg
     2521 ccggtcaggc cggactgaac gactccctca gccggaagtc gaggcggtcg cgcacggccg
     2581 gccagtcgaa gtcgcagatc gacagcacgg ccgtgtcgcg cacatggccg gtccaggtga
     2641 tcttgtgatt gcgcagcgtg ccttccttgg tcgcgccgag cttgaggacg gccgcctcgc
     2701 tgcgggcgtt gcgctcgtcg atcatgaatt ccacgcggat cgcgccggcg tcgaaggcct
     2761 gggccagcat cagccgcttg gactcgggat tgaccggccc tgaccgcacg tcggggtgca
     2821 ggaaggtcgc gccgatctcc acgcccttgt ggatccgacg gatgttcaga aagctcgagg
     2881 tgccgaccac ccgcttgtcc gaaagccggc ggatggcgta gccgatccgc tcgccgcgct
     2941 cggtctcgcc ctgcagcgcg ccccaccagt cggcgaaacc ctctccgcag ccgttggtcg
     3001 acatgatttc ccacgaggcg ggatcgcagt cgatggctcc gcgcagttcg tcgcgcaacg
     3061 cggcggtgat cggttcgagc cgcacatggc ggcctttcag gactttggcc ttgaggttca
     3121 tggcagccag cttgcccaag gccgcgacgg ttggaaacag cctgcggaaa attaatgtgg
     3181 cgccgttgaa aatttatgtc ttatataaaa agaacgacgt gccacatgga gcggccacaa
     3241 tgccgtacga cacctctgac aagtcccttc gtcggcaggc caccttcctg caggcggcca
     3301 cggtcatgat cgtggcggcc accgttctga actacctgcg caccggcgag gtgatcccct
     3361 tcctgttcgc cctgtttgga accggcctta cccaggtgga gatcgacggt cttctcaagg
     3421 ggctggccct ggcgttgccc ggcttcttct atgccggcgc ggtcctggtc gcgggcggca
     3481 tcttcggccg gatcgccacg ggcgagctct tcagcgcgcg caacagtgcg gcgctgggcg
     3541 ccgtcggcgg ctggctgtcg atgggcgcaa tcgtcgccat gatcgtgacg ccggtcgtgg
     3601 tcgactggct cgatctcggc gccctgcgct tgcggacaga gccgacggac atgctgatcg
     3661 cggtgatcgg cggcctgatc cagatcctcg gcttcgtcca cacccgggcg accgcgctgc
     3721 ggtccgaact cgaccagatc atctaggggg aattgtccat gtccaacgcc aagctggccg
     3781 atcaggccaa gacctttgaa gtcatcgcct ggatcgccgt cgtggtcctg tcgcttcagt
     3841 tcctggtgtt cctgaacctg ttgaccctga tcggaccgat cgccgacgcg atccggctcg
     3901 gcgatacgac cgggctgaga gacctgttcc agccggtgct gtaccggctg gtcgacgtcc
     3961 tcccggcgct gatctatctc gggggtgtct gggcggccgc ccggatcttt ggccgggtgg
     4021 ccaagggcga gctgttctcc cgcgcgaatt ccgcgggcct ggccgaggtc ggctcctccc
     4081 tgctgtgggg cgcggcggcc agcgccgtga tcgtgccctg gatccgcagc tggatcgccg
     4141 gtgactacgg attcagcggc atccatctgg aaatcgagac ctgggtcatc gccgtcatcg
     4201 gcggcgccat cctggttctc ggcaagatga tggcccaggc cggccgcctg cagtccgagc
     4261 tgagcgagat catctgacct gtgcccgtca tcgtcaccct cgacatcatg ctggcccgtc
     4321 gcaagatgaa ggcccgcgac ctggcccagc gtatcgggct gtcggagacc cagctcagcc
     4381 tgctgcggac cggcaaggtg cgggggatgc ggttcgacac cctgtccaag ctctgcgccg
     4441 tgctggagtg cacgccgggc gagttgctcg actaccggta tgatgagggt gacctggcgg
     4501 tggccgatcc tgaatagtcc tccccgcgac gcggggagga ttggtttgaa gcttgcccct
     4561 cgcgtcaccc gccgttacct tgcgctcaaa caaccgtttg aaagggcagg gccgacccat
     4621 ggacttcgat atctccccca agcagcgcca gttcctcgac cgcgtggtcg cgttcatgga
     4681 cgagcacatc gcgcccgccg tcccgcgcta caatgaagag atgaacgtca tcggcgacgc
     4741 ccgctggaag gtggtccagg tcgttgagga gttgaaggaa aaggccaagg ccgccggcct
     4801 gtggaacttc ttcatgccgc cgcacag
//
LOCUS       DAORNX010000212         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13235_length_4791_cov_6.085938, whole genome shotgun sequence.
ACCESSION   DAORNX010000212 DAORNX010000000
VERSION     DAORNX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_4791_cov_6.085938"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1714
                     /gene="aceB"
                     /locus_tag="PLO65_09980"
     CDS             128..1714
                     /gene="aceB"
                     /locus_tag="PLO65_09980"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRD28616.1"
                     /translation="MAAGFTFTEPLTEADREILTDEALAFLGELVDRFAARRDELLAA
                     RKAWQARIDAGGLPGFRPETASVREGDWKVGALPADLLDRRVEITGPAEAKMVINALN
                     SDVKLFMADFEDALSPTWANVIGGQRALRDAIRRTLTWDDPANGKHYALGDRLATLVA
                     RVRGLHLDEKHILRNGRKIPGALVDFGLYFHHNHAALTAIGSGPYLYVPKLEHFEEAR
                     WWNDVFVFAQQRHGVPVGTIKATMLIETLPAVFEMDEILFELRDHAAALNCGRWDYIF
                     SYIKTLRRHGDRLLPDRTSVSMDKPFLDAYSRLLIRTCHRRGALAMGGMSAFIPRKDP
                     DENAQVLERVRADKAREAANGHDGSWIAHPGLSDVVNAVYGAAFAPGRTNQLEVSRAN
                     DAPVTEADLLAPPPGPFTEAGLRHNQRVAVQYIEAWLGGSGAVAIYGLMEDAATAEIS
                     RASIWQWIRNGVTLDDGTPVTAELFERTFVEELAVVRAEVGEARWASGRWDDAADLMR
                     ELCLQEEFAEFLTLSAYERL"
     gene            complement(2075..2560)
                     /locus_tag="PLO65_09985"
     CDS             complement(2075..2560)
                     /locus_tag="PLO65_09985"
                     /inference="COORDINATES: protein motif:HMM:NF009932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRD28617.1"
                     /translation="MTPRPLTADAFAPYGEVISAREGVEQYPINHGATTRFNALAHAD
                     AATDGGAPILSIFRSTPLPEPVLKIMERHPLGSQAFMPLNGRPYLVAVAPAGDLDPAK
                     IEVFLASGDQGVNYAAGTWHHYSLALGEVSDFLVVDRQGPGANLDEVELSETDQIVVV
                     L"
     gene            complement(2569..3750)
                     /locus_tag="PLO65_09990"
     CDS             complement(2569..3750)
                     /locus_tag="PLO65_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRD28618.1"
                     /translation="MIADLLAWLNLGVRWLHVIAGIAWIGSSFYFMWLDARLKADATT
                     PPGVQGDLWAVHGGGFYHKRKYLSVPPDMPDELHWFKWEAYTTWLSGFALLGVIYYLG
                     APTYLIDPGKVAMAPWQAVAIGLAFLAGGWILYDALCRSPLGQRLTLFGLVWFLILTA
                     AAWALGHIFSNRGAFIHLGAIIGTVMAANVFMVIIPNQRKIVAAMLAGEPVDPALGKR
                     GKMRSVHNNYMTLPVLFLMVSNHYPMVIDHPLNWLLVAGIGLSGVTIRHFFNRRNAGA
                     SQPMILVGGALIFLLVMLVALATRPKAAEVAGVVPFREVQLIVRTHCIACHSASPTNP
                     AFASAPNGVAYDTPEEIRRYAPRIYERAVATDSMPLGNETGMTKAERDRLGAWIRAGA
                     K"
     gene            complement(3747..>4327)
                     /gene="xdhC"
                     /locus_tag="PLO65_09995"
     CDS             complement(3747..>4327)
                     /gene="xdhC"
                     /locus_tag="PLO65_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRD28619.1"
                     /translation="RGGLDEIDQWPGALLVGPDGAPLPGRRPRLGEGDAIIETVDARR
                     PRVLLFGAGHVGQAIARALAPLPFVLDWRDSRDEAAGPGVAVHGEAHLVDLAAAAGPG
                     DHVLILTHDHDLDYRLTHAVLTADKVRYCGLIGSDTKRARFLRRLRDDGVSEVAIARL
                     TCPIGLPGLKSKAPEVIAVAVAAQLLQMIETP"
     assembly_gap    4328..4565
                     /estimated_length=238
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4566..>4791)
                     /locus_tag="PLO65_10000"
     CDS             complement(<4566..>4791)
                     /locus_tag="PLO65_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRD28620.1"
                     /translation="PREAGARMLVGERAQSGTIGGGALEHRATDQARRMLAADRGWAL
                     QDYPLGPFLKQCCGGHVRLLLERLDGDSEGW"
BASE COUNT          711 a         1580 c         1589 g          673 t
ORIGIN      
        1 gtcatctccc actggatcgg tccccaggtc gcgcccgcga gatcggcgtt gaacggcatg
       61 gatggctccc cggcttgtcg cccctgtgcg caggggtcta tcaccgtcga ccattcagga
      121 gcgagacatg gccgcgggct tcaccttcac cgagccgctc accgaggcgg accgcgagat
      181 cctcaccgac gaggcgctcg ccttcctcgg cgagctggtc gaccgcttcg ccgcgcggcg
      241 cgacgagctg ctggctgccc gcaaggcctg gcaggcgcgg atcgacgccg gcggcctgcc
      301 gggctttcgt cccgagaccg cctccgtccg tgagggcgac tggaaggtcg gggcgctgcc
      361 ggccgacctg ctcgaccgcc gggtcgagat caccggtccg gccgaggcca agatggtcat
      421 caacgccctg aacagcgacg tgaagctgtt catggccgac ttcgaggacg ccctgtcgcc
      481 gacctgggcc aatgtcatcg gcggccaacg ggccctgcgc gacgccatcc gcagaaccct
      541 gacctgggac gaccccgcga acggcaagca ctatgcgctg ggcgacaggc tcgcgaccct
      601 ggtcgcgcgg gtgcgcggcc ttcatctcga cgaaaagcac atcctgcgga acggccggaa
      661 aatccccggc gcgctcgtcg atttcggcct ttacttccat cacaatcatg cggccctgac
      721 ggccatcggc agcgggccat acctctacgt gcccaagctc gagcacttcg aggaagcgcg
      781 ctggtggaac gacgtgttcg tcttcgccca gcagcggcac ggcgtcccgg tcggcacgat
      841 caaggcgacc atgctgatcg agaccctgcc ggcggtgttc gagatggacg agatcctgtt
      901 cgagctgagg gaccacgcgg cggcgctcaa ctgcggccgc tgggactaca tcttcagcta
      961 catcaagacc ctgcgccggc acggcgaccg gttgctgccg gaccgcacct cggtgagcat
     1021 ggacaagccg ttcctcgacg cctactcgcg cctgctgatc cgcacctgcc accgccgggg
     1081 cgccctggcc atgggcggca tgagcgcctt catcccgcgc aaggatccgg acgagaacgc
     1141 ccaggtgctg gaacgcgtgc gggccgacaa ggcgcgcgag gcggccaacg gccatgacgg
     1201 gtcgtggatc gcgcacccgg ggctgtctga cgtggtcaac gccgtctacg gggccgcctt
     1261 cgcgccgggc cggaccaacc agctggaggt ctccagagcc aacgacgcgc cggttaccga
     1321 ggccgacctg ctggcgccgc cgccgggccc gttcaccgag gccggcctgc gccacaacca
     1381 gcgggtcgcg gtgcagtata tcgaggcctg gctcggcggc tcgggcgcgg tggccatcta
     1441 cggcctgatg gaggacgcgg cgacggcgga gatcagccgc gcctcgatct ggcagtggat
     1501 cagaaacggg gtgacgctgg acgacggaac gccggtgacg gccgagctct tcgaacgcac
     1561 cttcgtcgag gaactggccg tggtccgcgc ggaggtcggc gaggcccgct gggcctccgg
     1621 ccgctgggac gacgcggcgg acctgatgcg cgagctttgc ctgcaggagg agttcgcgga
     1681 gtttttgaca ctgagtgcgt acgagcggct gtagccgggc tggccctcca attgacctcc
     1741 cccagtaggg ggagggggac catgcgaagc atggtggaga gggaggcgcg gtggcggacg
     1801 gcttcacact tcgctctcac cgaccggatg atttcggccc tttggccttg atggaatgat
     1861 gcgctccggg cggtcgtcct ggtcaaggac gggcccttcg gggtcgccgc tgcgcggccg
     1921 cgaagcggtc cttgagcagg ccgtccgccg gagcaacact ggccgtcaag acatccgggc
     1981 aggtctgccg cgacgtcgcg tcggccttcc tgccagcacg gcgcctccca ctccaccacc
     2041 cttcgggtgg tccccctctc ccaagtggga gaggttagag gacgacgaca atctggtcgg
     2101 tttcagaaag ctccacctcg tccaggttcg cacccggccc ttgccgatcc accaccagga
     2161 agtcgctcac ctcgcctagc gccagcgagt agtggtgcca tgtcccggcc gcgtagttca
     2221 cgccctggtc gccgctcgcg aggaacacct cgatcttcgc cgggtcgaga tcgccggccg
     2281 gggccacggc caccaggtag ggccggccgt tcaggggcat gaaggcctga ctgccgagcg
     2341 ggtggcgctc catgatcttc agcaccggct cgggcagggg cgtcgagcgg aagatcgaca
     2401 ggatcggcgc gccaccgtcg gtcgcggcgt cggcatgggc cagagcgttg aaccgcgtcg
     2461 tggcgccatg gttgatcgga tactgctcga ccccttcgcg cgcggagatg acctcgccgt
     2521 agggcgcgaa ggcgtccgcc gtcaggggcc ggggcgtcac gcgccgcatc acttcgcgcc
     2581 cgcccggatc caggcgccga gccggtcgcg ctccgccttc gtcatgcccg tctcattgcc
     2641 cagcggcatg ctgtcggtgg ccaccgcgcg ctcgtagatg cgcggggcgt agcggcggat
     2701 ttcctcgggc gtgtcatagg ccacgccgtt gggcgcgctg gcgaaggccg ggttggtcgg
     2761 gctggccgag tggcaggcga tgcagtgggt ccgcacgatg agctgcacct cccggaacgg
     2821 cacgaccccc gccacctcgg ccgccttcgg cctggtcgcc aacgccacca gcatcaccag
     2881 caggaagatc agcgcgccgc cgacgaggat catcggctga ctggcgccgg cgttccggcg
     2941 gttgaaaaag tgacggatgg tcacgccgct gaggccgatg ccggcgacca gcagccagtt
     3001 cagcggatgg tcgatgacca tcggatagtg gttgctgacc atcaggaaca gcacgggcag
     3061 ggtcatgtag ttgttgtgca ccgagcgcat cttgccgcgc ttgccgagcg ctgggtcgac
     3121 cggctcgccg gccagcatgg ccgcgacgat cttcctctgg ttgggaatga tcaccatgaa
     3181 gacgttggcc gccatcaccg tgccgatgat cgcgcccaga tggatgaagg ccccgcggtt
     3241 gctgaagatg tggccaagcg cccaggcggc ggctgtcagg atcaggaacc agaccaggcc
     3301 gaacagcgtg aggcgctggc ccagcggcga ccggcagagg gcgtcataga ggatccagcc
     3361 gccggcgagg aaggccaggc cgatggccac ggcctgccac ggcgccatcg ccaccttgcc
     3421 aggatcgatc aggtacgtcg gcgcgcccag gtagtagatc acccccagca gcgcgaagcc
     3481 gctgagccag gtggtgtagg cctcccattt gaaccagtgc agctcgtccg gcatgtccgg
     3541 cgggaccgag aggtatttgc gcttgtgata gaagccgccg ccgtggacgg cccacaggtc
     3601 gccctgaacc cccggcggcg tcgtcgcgtc ggccttcagc cgggcgtcga gccacatgaa
     3661 gtagaacgac gagccgatcc aggcgatgcc ggcgatgaca tgcagccagc gcacgccgag
     3721 attcagccag gccagaaggt ccgcgatcat ggcgtctcga tcatttgaag aagttgggcg
     3781 gccacggcca cggcgatcac ctccggcgcc ttgctcttca ggcctggcag gccgatcggg
     3841 caggtcaggc gggcgatggc gacctcgctg acgccgtcgt cgcgcaggcg gcgcaggaag
     3901 cgggcgcgct tggtgtcgga gccgatcagg ccgcagtatc tgaccttgtc ggccgtcagc
     3961 acggcatggg tcagacggta gtccaggtcg tggtcgtggg tcaggatcag cacatggtcg
     4021 ccgggcccgg ccgccgcggc gaggtcgacc agatgagcct cgccatgcac cgccacgccc
     4081 gggccggcgg cctcgtcgcg gctgtcacgc cagtcgagga cgaagggcag gggcgcgagg
     4141 gcccgggcga tggcctggcc gacatggccg gcgccgaaaa ggaggacgcg gggacgccgg
     4201 gcgtcgacgg tttcgatgat ggcgtcgcct tcgccgagcc gcggccggcg gcccggcagc
     4261 ggcgcgccgt ccggaccgac gagcagggcg cccggccatt gatcgatttc atccaatcct
     4321 ccccgcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnccagc cttcgctgtc cccgtccagc cgctccagca gcagccgcac atggccgccg
     4621 cagcactgtt tcaggaacgg ccccagcggg tagtcctgca aggcccatcc ccggtccgct
     4681 gccagcatcc gccgcgcctg gtccgtcgcc cggtgctcca gcgccccgcc gccgatggtg
     4741 ccgctctgcg cccgctcccc caccagcatc cgcgccccgg cctcgcgcgg g
//
LOCUS       DAORNX010000213         4777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13269_length_4777_cov_7.740364, whole genome shotgun sequence.
ACCESSION   DAORNX010000213 DAORNX010000000
VERSION     DAORNX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13269_length_4777_cov_7.740364"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..107)
                     /locus_tag="PLO65_10005"
                     /pseudo
     CDS             complement(45..107)
                     /locus_tag="PLO65_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(490..2544)
                     /locus_tag="PLO65_10010"
     CDS             complement(490..2544)
                     /locus_tag="PLO65_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28621.1"
                     /translation="MTLIATGGPLSTILDAIARYVEAEHPRMLCSILLLDDAGERLMF
                     GAGSSLPDFYNAAIDGIAIGPGVGSCGTAAYTNACVVVADIQADPLWADFRELAGQAG
                     LASCWSEPIRGRDGQVLGTFAMYHRDVRVPAEDDFRSIEAAAHLAAIAIERKRSDDAL
                     AASEARHRLFADNATDLIMLSDREGRLTYISPSVARVTGYLPEMLVGRLIEDMVHPED
                     WPAMKAIGDRLYAGEFPGDTPRVDYRARHKDGRWLWFETRTGLIRDPATGEPTGTLDV
                     AREVTARKTLEAELRERTREAEAATEAKSNFLANMSHEIRTPLTAILGFSGLLRRLPG
                     LPGEAGQHLQRITTAGEALLSVVNDVLDFSRLEAGQIAMDPQPFDPAAVVRETVDLVA
                     GQAAAKGLGLSVDLDAELPAAVRADSARLRQILLNLLGNALKFTQAGDVRVTVRHRPD
                     EGGQLRIEVADTGPGVPADRLDRLFQRFSQVDGSISRQHGGSGLGLAICLGLTELMGG
                     RIGVDSVEGEGSRFWFTIAAPPAAGHAGAGPAPDAVETMPAARLLLVDDVAINRQLVR
                     AILAPFGHLITEAGGGAEAVEVARLSDFDLILMDVQMPGMDGLSATREIRAEAGPNRH
                     TPIIAFSANVLADQLAACRAAGMNDHVAKPIDLAELVAKVALWTAEAGLEKAAADYVR
                     LA"
     gene            complement(2794..3936)
                     /locus_tag="PLO65_10015"
     CDS             complement(2794..3936)
                     /locus_tag="PLO65_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28622.1"
                     /translation="MDFNFTEEQSMLRDTVTSFLQDRYDFDKRRKMITSEAGRDPAIW
                     EAFANELGILGAPFSEELGGLGGGAIENMIVMEEFGKALVVEPYLGTVVIGGGFLKHS
                     GYAGAADLIGGIIEGKTIIAFAYAEPQGRYTWSDLKTTAKKDGSGYVLNGHKAVVTGA
                     PWATHLIVTARTGGGQRDASGVSVFIVDKNAKGVVTRDYPTVDGQRASEVYFENVSVG
                     ADALIGAEGAGLPLVEKVLDEATAAACAEAVGAMRQLHTGTLEYAKQRKQFGVPIASF
                     QVLQHRMVDMFMNVEQSVSMTYMATIKLNDDAERAKAVSAAKVQIGKACKFVGQNAIQ
                     IHGGMGMTDELAIGHYFKRATMIEGLYGSVDHHLRRYEGLSFGKAA"
     gene            complement(3974..>4777)
                     /locus_tag="PLO65_10020"
     CDS             complement(3974..>4777)
                     /locus_tag="PLO65_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28623.1"
                     /translation="GSDLASLKTKAERITGDDGKEYYLVNGQKTWTTLAQHADWGFFL
                     VRTNPDAKIQEGISFLLIDMKSPGIEVRRITTLEGGHEVNEVWLENVKVPVENRVYEE
                     NQGWTCAKFLLAHERSGIAGVARSKRGVEKIREMASTELGDDGKPLIQDQDFKRKVAE
                     LEIDLTALEFTELRTLASESAGKGPGPESSLLKIKGTEIQQRLTELALEASGHYGAPY
                     LRDLGGNARIGPDHAVGRAGAYFNTRKTSIYGGSNEIQRNIIAKMVLGL"
BASE COUNT          694 a         1635 c         1627 g          821 t
ORIGIN      
        1 ggcctcgccg gagaccggcc gccgcccggg ttcctcggtc gcggcacaag catcaacccc
       61 agcagtccga atgacagcat ggcgtggtcc atcttcgata tcgtcatgtc ggcgtctcct
      121 cgagccgtga gccgaaagag acgcggagcg gccttggggg tctgtcgtgg cgatgccctc
      181 ggggtcgtca gcgcgtcgag atcaagcctg cgccggccga gtcgcgggac gcaagaaaat
      241 taatccgctt tcggactatt tccgtatgcg gattatcggg cattcttttt tcttttcagt
      301 attttactgg ccaatcaccg acggttgatg acctgtatat ccgtcaccct gggcgccgtc
      361 gccccgccat ggagttcgac cgtgaccgcg ggaacgacaa ccggccccgc gtcccctcgc
      421 ccggacggtc cggccgggcg accgccagtc tcgaaccgag gcggcgccga ggcccctggc
      481 gaccggcgac tacgccaggc ggacgtaatc cgcggccgcc ttctcgagac cggcctcggc
      541 agtccacagg gcgaccttgg cgaccagttc cgccaggtcg atcggcttgg cgacgtgatc
      601 gttcataccg gcggcccggc aggccgccag ctggtcggcc agcacattgg cgctgaaggc
      661 gatgatcggc gtgtgccggt tgggtccagc ctcggcccgg atctcccggg tcgcgctcag
      721 gccatccatg ccgggcatct gcacgtccat caggatcagg tcgaagtccg agaggcgcgc
      781 gacctcgacc gcttccgcgc cgcccccggc ctcggtgatc aggtgtccga acggcgccag
      841 gatggcccgc accagctgcc ggttgatcgc gacgtcgtcg accagcagca ggcgggccgc
      901 cggcatggtc tcgaccgcgt ccggcgctgg ccccgcgccg gcatggccgg cggcgggcgg
      961 ggcggcgatg gtgaaccaga accgcgaccc ctcgccctcg acactgtcga cgccgatccg
     1021 gccgcccatc aactcggtca ggcccaggca gatcgccagc cccagccccg acccgccatg
     1081 ctgcctgctg atcgagccgt cgacctgcga aaaccgctgg aacagccggt ccaggcggtc
     1141 ggccggcacg cccggccccg tgtcggcgac ctcgatgcgc agctgtccgc cctcatcggg
     1201 ccggtgccga accgtcaccc ggacatcgcc cgcctgggtg aacttcaggg cgttgccgag
     1261 cagattgagc aggatctggc gcagccgtgc gctgtcggcc ctgaccgcgg cgggcagctc
     1321 ggcgtccagg tcgacggaca gtcccagccc cttcgccgcc gcctgcccgg ccacgaggtc
     1381 gacagtctcg cggacgacgg ccgccgggtc gaacggctgg ggatccatcg cgatctgtcc
     1441 ggcctcaagc cgggagaagt cgagcacgtc attgaccacc gaaagcaggg cctcgcccgc
     1501 cgtggtgatc cgctgcagat gctgccccgc ctcgcccggc aggccgggca atcgccggag
     1561 caatcccgaa aagccgagaa tcgccgtcaa cggcgtgcgg atctcgtgac tcatgttggc
     1621 caggaagttc gacttggcct cggtcgccgc ctcggcctcg cgggtccgct cgcgcagttc
     1681 ggcctcgagc gtcttgcgcg ccgtcacttc gcgggcgaca tccagcgtgc cggtcggctc
     1741 acccgtcgcg ggatcgcgga tcaggccggt gcgggtctcg aaccacaacc agcgcccgtc
     1801 cttgtggcgc gcgcgatagt ccacccgcgg cgtgtcgccg ggaaactcgc cggcgtacag
     1861 gcggtcgccg atggccttca tcgccggcca gtcttcgggg tggaccatgt cctcgatcag
     1921 ccgcccgacc agcatttccg gcagatagcc cgtcaccctg gccacggacg gcgagatgta
     1981 ggtcaggcgt ccctcgcgat cactgagcat gatcaggtcg gtggcgttgt cggcgaacag
     2041 gcggtggcgg gcctcgctgg ccgccagggc gtcgtcggac cgcttgcgct cgatggcgat
     2101 ggccgccaga tgcgcggccg cctcgatcga gcggaagtcg tcctccgcag gaacccggac
     2161 gtcgcgatga tacatggcga aggtgccgag cacctggcca tcgcgccccc ggatcggctc
     2221 ggaccagcag gacgccagtc cggcctggcc ggccagctcc ctgaagtcgg cccacagcgg
     2281 gtccgcctgg atgtccgcga ccacgacgca ggcgttggtg taggccgccg tgccgcagga
     2341 tcccacgccg ggcccgatgg caatgccgtc gatggccgcg ttgtagaagt ccggcaggct
     2401 cgatccggcg ccgaacatca gccgctcgcc ggcgtcgtcg agcagcagga tgctgcacag
     2461 catgcgcgga tgctcggcct cgacatagcg ggcgatcgcg tcgaggatcg tcgacagcgg
     2521 cccccccgtc gcgatcagcg tcatggtccg cgtcagggtc gcggtcctcg attcacgatc
     2581 gacggcggac tgccgcgccg tctctgctgc gagctgatcg accatgggag cacggaaccg
     2641 ccgcgtcctt cgatgaggcc gcgtggccaa tctggaggca cagcgatttc agattggtga
     2701 atcaccgttt tccgaccgcg gcagggccga cagacaaagg gcggagcctt ccggccccgc
     2761 cccctggttc ccgtgaatgc gcttgccggc gcgctaggcc gccttgccga agctcaagcc
     2821 ctcgtagcgg cgcaggtgat ggtcgaccga gccgtagagg ccctcgatca tcgtggcgcg
     2881 cttgaagtag tggccgatgg ccagttcgtc ggtcatgccc atgccgccgt ggatctggat
     2941 ggcgttctgg ccgacgaact tgcaggcctt gccgatctgc accttggcgg cgctgacggc
     3001 cttggcccgc tcggcgtcgt cgttgagctt gatggtggcc atgtaggtca tcgacaccga
     3061 ctgctcgaca ttcatgaaca tgtcgaccat gcggtgctgc agcacctgga agctggcgat
     3121 cggcacgccg aactgcttgc gctgcttggc gtattcgagg gtgccggtgt gcagctggcg
     3181 catggcgccg accgcttcgg cgcaggccgc ggcggtggct tcgtccagca ccttctcgac
     3241 cagcggcagg cccgcgcctt ccgcgccgat cagggcgtcg gcgccgaccg agacgttctc
     3301 gaaatagact tccgaggcgc gctggccgtc gacggtcgga tagtcgcggg tgaccacgcc
     3361 cttggcgttc ttgtcgacga tgaacaccga cacgcccgag gcgtcgcgct ggccgccgcc
     3421 ggtgcgggcg gtgacgatca ggtgggtggc ccagggggcg cccgtgacga ccgccttgtg
     3481 gccgttcagg acatagccgg agccgtcctt cttggccgtg gtcttcaggt ccgaccaggt
     3541 gtagcggccc tgcggctcgg cataggcgaa ggcgatgatc gtcttgccct cgatgatgcc
     3601 gccgatcagg tcggccgcgc cggcgtagcc cgagtgcttg aggaagccgc cgccgatcac
     3661 caccgtgccg aggtagggct cgacgacgag cgccttaccg aactcctcca tgacgatcat
     3721 gttctcgatc gcgccgccgc ccaggccgcc cagctcttcg gagaaaggcg cgccgaggat
     3781 gcccagctcg ttggcgaagg cctcccagat ggccggatcg cggccggctt ccgacgtgat
     3841 catcttgcga cgcttgtcga agtcgtagcg gtcctgaagg aagctggtga ccgtgtcacg
     3901 cagcatcgac tgttcttcgg tgaagttgaa atccatgggt cgtccctgat cgtgttctgg
     3961 tttggggcga ggcctagagg cccagcacca tcttggcgat gatgttgcgc tggatctcgt
     4021 tggatccacc gtagatcgag gtcttgcggg tgttgaagta ggcgccggcg cggccgacgg
     4081 cgtggtcggg gccgatgcgg gcgttgccgc ccagatcgcg caggtagggc gcgccgtagt
     4141 ggccggacgc ctccagcgcc agctcggtca ggcgctgctg gatctcggtg cccttgatct
     4201 tgagcaggct ggattccggc ccgggtccct tgccggcgct ctcgctggcc agggtgcgca
     4261 gttcggtgaa ctccagcgcc gtcagatcga tctcaagctc ggcgaccttg cgcttgaaat
     4321 cctggtcctg gatcagcggc ttgccgtcgt cgcccagctc cgtcgaggcc atctcgcgga
     4381 tcttctcgac gccgcgcttg gagcgtgcca cgccggcaat gccggagcgt tcgtgggcca
     4441 gcaggaactt ggcgcaggtc cagccctggt tctcctcgta gacgcggttc tcgaccggca
     4501 ccttgacgtt ctcgagccag acctcgttga cctcgtggcc gccttccagg gtcgtgatgc
     4561 gccgcacctc gatgcccggg ctcttcatgt cgatcagcag gaaggagatg ccttcctgga
     4621 tcttggcgtc ggggttggtg cggaccagga agaagcccca gtcggcgtgc tgggccaggg
     4681 tggtccaggt cttctggccg ttgaccaggt agtattcctt gccgtcgtcg ccggtgatcc
     4741 gctcggcctt ggtcttcagc gaggccaggt cggagcc
//
LOCUS       DAORNX010000214         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13429_length_4738_cov_7.177664, whole genome shotgun sequence.
ACCESSION   DAORNX010000214 DAORNX010000000
VERSION     DAORNX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_4738_cov_7.177664"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PLO65_10025"
     CDS             complement(<1..137)
                     /locus_tag="PLO65_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD28624.1"
                     /translation="MSRPPEFLPLSDAAVRAVAGPGGRHAALIEDAFKVLLETPGGGV
                     SL"
     gene            complement(134..1477)
                     /gene="miaB"
                     /locus_tag="PLO65_10030"
     CDS             complement(134..1477)
                     /gene="miaB"
                     /locus_tag="PLO65_10030"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRD28625.1"
                     /translation="MTDAAPQKRLYIKTYGCQMNVYDSERMADVLRPLGYAVTDEPEG
                     ADLVVLNTCHIREKATEKVYSELGQIKLMKSRKAQAGGAMTIAVAGCVAQAEGEEIMR
                     RQPAVDLVVGPQAYHQLPELIARAHRATGERLSADFAADDKFDALPKDRHVTGVTAFL
                     TVQEGCDKFCTFCVVPYTRGGEWSRPADDVVAEAEVLAARGVREVTLLGQNVNAYDGC
                     DGGLAGLARRLAKVEGLDRIRYTTSHPRDMDEALIAAHGELPELMPFLHLPVQSGSDR
                     ILKAMNRAHTAESYIRLIERIRAARPDMAISGDFIVGFPGERDSDFEATLNLVREVGY
                     ASAFSFKYSRRPGTPASAMPGQVATAVMDERLQRLNALLQEQQEAFNRSVVGRVEPVL
                     IEKAGRHPGQVGGRSPWLQPVHIEGPERLIGQILPVRIDGATRNSLAGSLVLEAV"
     gene            complement(1556..3961)
                     /locus_tag="PLO65_10035"
     CDS             complement(1556..3961)
                     /locus_tag="PLO65_10035"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HRD28626.1"
                     /translation="MTSGPGRIAIRLLGAAMILIGLVLSVGGAWLAALGGSPYYLVAG
                     LGIIISAVLMLRGSLTGVWVYLAVYGLTWIWAIWEVGLAGWPLVPRVVAPTVLLVLVI
                     ACVPVLRPTPAGRRGGLAGLAATIALAIVAGIGVGIANRAPQALPLPDAGLPTPADAS
                     PLPVGADWAAYGGTYGGRRYSPLTQINAQNVGRLKRVWLSHTGDMPSAAARDKYGAET
                     TPLKVGDTLYLCSAKNILIALDPVTGRTRWRHDPQVADKYIPYTAACRGVSYYAVPGA
                     AADEPCGARIFEGTLDGRIIAVDAHTGRSCAGFGHRGEVRITDGMGAVTPGMVSITSA
                     PTIVRGVVVTGHQVLDGQKRYAPSGVIQGYDAVTGELRWAWDMVRPDRTDAPPPGEVY
                     TRGTPNMWSTATGDEALGLVYLPLGNSAADYWSGLRRPEEKAYSSSLVALDVTTGKPV
                     WSFQTVHNDVWDYDLGSPGTLVDFPTAGGATPAIILPSKQGDIYILDRRTGQPLTPVS
                     ERRVPGGGVEPEQRSPTQPYSGYHTLRRPDLLERDMWGMSPIDQMICRIQFRRASYDG
                     FYTAPTSDRRWIEYPGYNGGSDWGGVAVDPRRGVIVANYNDTPNYNRLVPRAEADAKG
                     WTPRGDARGGSMGHGAEGAGDPQAGTPYAITVNAGWRMPATGLMCKEPPYGGIRAIDL
                     RTGRTLWDRPLGTARRNGPFGIPSMLPINIGTPNNGGALVTAGNLVFVAAATDNLIRA
                     IDIRTGETVWSDVLPAGGQATPMTYEAGGRQFVVIMAGGHHFMETPIGDEVVAYALPG
                     SGT"
     gene            complement(4088..4495)
                     /locus_tag="PLO65_10040"
     CDS             complement(4088..4495)
                     /locus_tag="PLO65_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRD28627.1"
                     /translation="MDRLEKLCVEKGMRMTDQRRVIARVLSGAADHPDVEELYRRAHE
                     IDPHISIATVYRTVRLFEESGIIERHDFRDGRSRYEEAPDVHHDHLIDMKSGKVVEFV
                     DQEIEALQQAIARKLGYRLVDHRLELYGVPLED"
     gene            complement(4539..>4738)
                     /locus_tag="PLO65_10045"
     CDS             complement(4539..>4738)
                     /locus_tag="PLO65_10045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28628.1"
                     /translation="GRVLVEAAAATAAARGADSLFLEVSADNAAALALYAAAGFEPAG
                     LRKGYYADGADALVLRRTLNS"
BASE COUNT          676 a         1635 c         1707 g          720 t
ORIGIN      
        1 agcgacacgc cgccgccggg cgtctcgagc aggaccttga aggcgtcctc gatcagggcc
       61 gcgtgacggc cgccggggcc ggccacggcg cgcacggcgg cgtccgacag cgggaggaat
      121 tcgggcgggc ggctcatacc gcttccagaa ccagtgagcc ggccaagctg ttccgcgtgg
      181 cgccgtcgat gcgcaccggc aagatctggc cgatcagccg ctcgggcccc tcgatgtgca
      241 cgggctggag ccacgggctg cgaccgccga cctggcccgg atggcggccg gccttctcga
      301 tcagcaccgg ctcgacgcgg ccgaccaccg agcggttgaa ggcctcctgc tgctcctgca
      361 gcagggcgtt gagccgctgc agccgttcgt ccatcaccgc ggtcgccacc tggcccggca
      421 tggcgctggc cggggtgccg ggccggcggc tgtacttgaa cgagaaggcg ctggcgtagc
      481 cgacctcgcg cacgaggttc agggtcgcct cgaaatcgct gtcccgctcg ccggggaagc
      541 cgacgatgaa gtcgccgctg atggccatgt cgggccgggc ggcgcggatc cgctcgatca
      601 gccggatata gctctcggcc gtgtgggcgc ggttcatcgc cttgaggatc cggtcgctgc
      661 ccgactgcac cggcaggtgc aggaagggca tcagctcggg caactcgcca tgggccgcga
      721 tcagggcctc gtccatgtcg cgcgggtggc tggtggtgta gcggatgcgg tcgaggccct
      781 cgaccttcgc cagccgccgc gccaggcccg ccagcccgcc gtcgcagccg tcataggcgt
      841 tgacgttctg gcccagcagg gtgacctcgc gcacgcccct ggccgccagc acctccgcct
      901 cggcgaccac gtcgtcggcc ggccgcgacc actcgccgcc gcgggtgtag ggcaccacgc
      961 agaaggtgca gaacttgtcg cagccctcct gcacggtgag aaacgcggtc acgcccgtga
     1021 catggcgatc cttcggcagg gcgtcgaact tgtcgtcggc cgcgaagtcg gcgctgagcc
     1081 gttcgccggt ggcgcggtgg gcccgggcga tcagctcggg cagctggtga taggcctgcg
     1141 gtccgacgac cagatccacg gccggctgac ggcgcatgat ctcctcgccc tcggcctggg
     1201 ccacgcagcc ggcgacggcg atggtcatgg cgccgcccgc ctgcgccttg cggctcttca
     1261 tcagcttgat ctggccgagc tcggaataga ccttctcggt ggccttttcg cggatatggc
     1321 aggtgttgag caccaccagg tccgccccct cgggctcgtc ggtcacggca tagcccagcg
     1381 ggcgcaggac atcggccatg cgctcgctgt catagacgtt catctgacag ccgtaggtct
     1441 tgatgtagag gcgcttctgc ggcgcggcgt cggtcatcga gcggttatgg ggtcgcggtc
     1501 atcgtcgtgc aacctccgcc cgtcccggac cggcgagggc gggggcccgc cgcgatcagg
     1561 tccccgatcc cggcagggcg taggcgacca cctcgtcgcc gatcggcgtc tccatgaaat
     1621 ggtgcccacc ggccatgatg accacgaact ggcgtccgcc cgcctcatag gtcatcggcg
     1681 tcgcctgccc gccggccggc agcacgtcac tccagaccgt ctcgcccgtg cggatgtcga
     1741 tggcgcggat caggttgtcg gtcgccgcgg cgacgaacac caggttgccg gccgtcacca
     1801 gcgcgccgcc gttgttgggc gtgccgatgt tgatcggcaa catcgacggg atgccgaacg
     1861 gcccgttgcg tcgcgccgtg ccgagcggac ggtcccagag ggtgcgaccg gtgcgcaggt
     1921 cgatcgcgcg gattccgccg tagggcggct ccttgcacat caggcccgtc gccggcatgc
     1981 gccatccggc gttgacggtg atggcgtagg gtgtcccggc ctggggatcc ccggcgccct
     2041 cggcgccgtg acccatgctg ccgccgcgag cgtcgcctcg cggggtccag cctttcgcat
     2101 cggcttcggc gcgcggcacc aggcggttgt agttgggggt gtcgttgtag ttggcgacga
     2161 tgacgccgcg ccggggatcg acggccacgc cgccccagtc cgagccgccg ttgtagcccg
     2221 gatactcgat ccagcgccgg tcgctggtcg gggcggtgta gaagccgtcg tagctggccc
     2281 ggcggaactg gatgcggcag atcatctggt cgatcggcga cataccccac atgtcgcgct
     2341 cgagcagatc gggccgccgc agggtgtggt agccggaata gggctgggtt ggggaccgct
     2401 gctccggctc gacgccgccg ccgggaacgc gacgctcgct gaccggggtc aggggctgtc
     2461 ccgtgcgcct gtcgagaatg tagatgtcgc cctgcttgct cggcaggatg atcgccgggg
     2521 tcgcgcctcc ggctgtcggg aaatcaacca gcgtgccggg ggagcccagg tcgtagtccc
     2581 agacgtcgtt gtgcacggtc tggaacgacc agacaggctt gcccgtcgtg acgtcgaggg
     2641 cgaccagcga gctggaatag gccttctctt cgggtcgccg cagcccgctc cagtagtcgg
     2701 cggcggaatt tcccagcggc aggtacacaa ggccaagggc ctcgtcgccg gtcgccgtgc
     2761 tccacatgtt gggggtgccg cgcgtgtaga cctcgccggg cggcggcgcg tccgtgcgat
     2821 ccgggcgaac catgtcccag gcccagcgca actcgccggt caccgcgtcg tagccctgga
     2881 tcacgcccga gggcgcgtag cgcttctgcc cgtcgaggac ctggtggccg gtgaccacca
     2941 cgccccggac gatggtcggc gcagaggtga tcgacaccat gccgggggtg acggcgccca
     3001 tgccatcggt gatgcgcacc tcgccgcggt gaccgaatcc ggcgcacgag cgcccggtgt
     3061 gggcgtcgac ggcgatgatc cggccgtcca gcgtgccctc gaagatgcgg gcgccgcacg
     3121 gttcatccgc cgccgccccg gggacggcgt agtaggagac gccccggcaa gccgccgtat
     3181 aggggatgta cttgtccgcg acctgcgggt cgtggcgcca gcgggtcctg ccggtcaccg
     3241 ggtccagcgc gatcaggatg ttcttggcgc tgcagagata cagggtgtcg ccgaccttca
     3301 gcggggtggt ctcggcgccg tacttgtccc tggcggcggc gctcggcatg tcgccggtgt
     3361 ggctgagcca cacgcgcttc agccgcccga cattttgagc gttgatctgg gtcagcggcg
     3421 aatagcgtcg gccgccgtag gtcccgccat aggcggccca gtcggccccg accggcagcg
     3481 gcgaggcgtc ggccggcgtc ggcaaccccg cgtccggcag cggcagggcc tgcggcgcgc
     3541 gattggcgat gcccacgcca atgcccgcaa caatggccaa ggcgatggtg gccgccagtc
     3601 cggccagtcc accgcgccgc cccgccgggg tcggcctgag gacgggaaca caggcgatga
     3661 cgaggaccag cagcacggtg ggggcgacga cccggggaac cagcggccag ccggcgagcc
     3721 caacctccca gatcgcccag atccaggtca ggccgtagac ggccagatag acccacacgc
     3781 cggtcagcga cccgcggagc atcagcaccg ccgagatgat gatgccgagg ccggcgacca
     3841 ggtagtaggg agacccgccg agcgccgcga gccaggctcc gcccactgac aagacaagac
     3901 cgatcaggat catggcggcg ccgaggagac ggatcgcgat ccgccccggc ccggaagtca
     3961 tattggtcac agcaccctct ggcccgatca atcgggcagg ctcgcgttat ccccgcctta
     4021 cggttgggta accgacagag ggtgtggccg gttcccgatt tccccgtgaa ggacggtccg
     4081 gccgggccta gtcttcgagc ggcaccccgt agagctccag ccggtgatca accagccggt
     4141 agccgagctt gcgggcgatg gcctgctgca gggcctcgat ctcctggtcg acgaactcga
     4201 cgaccttgcc gctcttcatg tcgatcaggt ggtcgtggtg gacgtccggg gcttcctcgt
     4261 agcggctgcg gccgtcgcgg aaatcatgcc gctcgatgat gcccgactcc tcgaacagcc
     4321 gcaccgtgcg gtagaccgtg gcgatggaga tgtgcgggtc gatctcgtga gcgcggcggt
     4381 agagctcctc cacgtcagga tgatcggcgg cgccggacag cacgcgggcg atcacgcgcc
     4441 gctggtcggt catccgcatc cctttttcga cgcagagctt ttcgaggcgg tccacggggt
     4501 tctcctgacg agtcgcgggc aggataggcc cgccgggctc agctgttaag cgtgcgccgc
     4561 aggaccaagg cgtcggcccc gtccgcgtag tagcccttgc gcagtccggc cggctcgaac
     4621 ccggcggctg cgtaaagcgc gagcgccgcg gcgttgtcgg cggacacctc gagaaacagg
     4681 ctgtcggccc cgcgcgcggc ggccgtggcg gcggcggcct cgaccaggac gcggccga
//
LOCUS       DAORNX010000215         4722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13488_length_4722_cov_7.177844, whole genome shotgun sequence.
ACCESSION   DAORNX010000215 DAORNX010000000
VERSION     DAORNX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13488_length_4722_cov_7.177844"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..862)
                     /locus_tag="PLO65_10050"
                     /pseudo
     CDS             complement(1..862)
                     /locus_tag="PLO65_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515935.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
     gene            complement(1074..1418)
                     /locus_tag="PLO65_10055"
     CDS             complement(1074..1418)
                     /locus_tag="PLO65_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRD28629.1"
                     /translation="MTRIYKLLTRPEWLAASDAGVFTGSPVDLADGFIHFSTAAQAPE
                     TGRRYFSGVEGLVAVAFEAEALGVALKWEPSRGGDLFPHLYGPLPTALAVEAMPVELD
                     ADGVPLLAGLKA"
     gene            complement(1415..1924)
                     /locus_tag="PLO65_10060"
     CDS             complement(1415..1924)
                     /locus_tag="PLO65_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD28630.1"
                     /translation="MKRLILMRHGKAERSSPGGDFERALTDRGQADAALMGRLLLDEG
                     LVPDVGLVSAARRTRQTWEQVSLSFPAAAVRPLGGLYHADPGRILFEAEAAGVEHDTV
                     MVVGHNPGIHALTVALLNEGGAGSSLVSRAESRFPTSTVAAFTFDPAGRPVYDGLFYA
                     ADHGGGGGE"
     gene            2005..3156
                     /locus_tag="PLO65_10065"
     CDS             2005..3156
                     /locus_tag="PLO65_10065"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRD28631.1"
                     /translation="MTLEASSEIEQHGGVWRFPADQPLLLDSGGRIEGLEVAWTSYGR
                     LNADRSNAVLICHALTLDQHVAATHPVTGKPGWWTRLVGPGRPLDPARHYILCANVVG
                     GCMGTTGPGSINPVTGRPWGLTFPVVTIADMVRAQAMLVEALGIDTLLAVVGGSMGGM
                     QVLQWAASYPERMFSAVCVAAAARHSAQNIALHEVGRQAIMADPDWRGGDYALHGTRP
                     EKGLAVARMAAHITYLSEAALQRKFGRELQRDGLSWGFDADFQVESYLRHQGASFVDR
                     FDANSYLYITRAMDYFDLAAAHGGVLAEAFRGARHVRFCILSFTSDWLYPTAESRHIV
                     RALNAAGARASFVEIESDKGHDAFLLDEPVMDSALAGFLDAVGTARGLT"
     gene            3153..3812
                     /gene="metW"
                     /locus_tag="PLO65_10070"
     CDS             3153..3812
                     /gene="metW"
                     /locus_tag="PLO65_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRD28632.1"
                     /translation="MTAVREDFREILRLVRPGARVLDVGCGGGELLEMLARDKQVDGR
                     GVEISTQGVSACLARGLAVMQGDADRDLDQFPAGAFDYAVLSQTLQATRNPRQVLSEL
                     LRIADRAVVSFPNFGHWRVRWSLLSRGRMPETRSLPEPWWSTPNIHLCTLADFNALVA
                     DLDVRIEACAALSEGRPARPMNPTSLLENWRAEAALFLLARRNAEPAEPIDAPRDLFG
                     G"
     gene            3824..4576
                     /locus_tag="PLO65_10075"
     CDS             3824..4576
                     /locus_tag="PLO65_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRD28633.1"
                     /translation="MTYNVHRCVGVDRKLNVERVAEVIAACRPDIVALQELDVGRART
                     RGVDQAHRLAELLQMRAHFNAALTVEEERYGDAILTALPERLVKVGPLPGLPRLRGLE
                     PRGALWVAVDLDGRELQVINTHLGLVPREQQVQVAELLGRRWMGAEGFESPAVLLGDF
                     NATPFSQTYRMLTAVLRDAQDGGPKAATATFPSGFPMLRIDHVFVSSEIRVRRVESPF
                     DPRARAASDHLPLVVEIEVPAARAEQSATAGP"
BASE COUNT          656 a         1670 c         1742 g          654 t
ORIGIN      
        1 tccgcccgcg ccgatcagga ccaggccgcg gccggcgccc gcctcgtgga accggcccag
       61 catgcgggtc gacagatcca tcagcggcgg acccgacagg ccgccggctt cggcgcgcag
      121 gggcgagcgc aggccggccg gccgggagat tgtcgtgttg gagacgatga tcccctgcag
      181 gccgtaggcc gccaccgtct cgacgatggc ctcgatctcg tcgtcctcga ggtcgggggc
      241 gaccttgagg aacacgggac agtcgccggc gagggcgcgg cgggtccagc cgtccgagca
      301 gctcctcgag ggcggcgcgc gtctgcaggc cacgcaggcc gggggtgttg ggcgaggaga
      361 tgttgaccgt gaaccagtcc gccaggcccc acaggcggcg caggcccagc acatagtcgc
      421 caatccggtc ggcggagtcc ttgttggctc cgatattggc gccgaccggc cccacgcgcc
      481 gccgggccag gcgggcggcg aacgggccga gaccggcgtt gttgaagccc atgcggttga
      541 tcaccgcccg atcctcggtc agccggaaca gccggggccg cgggttgccg gcctgggcca
      601 ggggcgtgac cgtgccgcat tcgacgaagc cgaagccggc ccgcagcatg gccgccggga
      661 cctcggcgtt cttgtcgaag ccggcggcca ggccgatcgg cgaggccagc gggattccgg
      721 cgagcgtcgt ggtcaggatc gggtcggggg gcgtccggtc gacggggccg aggccgagcc
      781 tcaggcccag gatcgtggcg ccgtgggcgt cctcgggatc gagggcatgc agggcgcggg
      841 cgagggtgtc gtggagggtc atgcgccaac accctgtctt ctcccttccc cctggagggg
      901 ggaagggccg gggatggggg tggaggtctg tccacgccag cggtgtgcgc gggcggagcc
      961 ttctcctgtt tcaccgcacc ggctgaacct gcgccacccc catccctgac ccttcccccc
     1021 tccagggaga aggggacgac ggtcggaagg cgacgtacct gcttctggcg ccatcacgcc
     1081 ttcaaccccg ccaggagcgg aaccccgtcc gcgtcaagct ccaccggcat ggcctccacg
     1141 gccagcgcgg tcggcagcgg gccgtagagg tgcgggaaca ggtcgccgcc gcgcgacggc
     1201 tcccacttca gggccacgcc cagggcttcg gcctcgaagg ccacggccac caggccctcg
     1261 acgccggaga agtagcggcg gccggtctcc ggggcctggg cggcggtgga gaagtggatg
     1321 aaaccgtcgg ccagatcgac cggcgatccg gtgaacacgc cggcgtccga ggcggccagc
     1381 cattctggac gggtgagcag cttgtagatc cgcgtcactc gccgccgcct ccgccatggt
     1441 cggccgcgta gaacaggccg tcgtagaccg gccggccggc cggatcgaag gtgaaggccg
     1501 cgaccgtcga ggtcgggaac cggctctccg cccgggacac cagcgacgac ccggcgccgc
     1561 cttcgttcag cagggccacg gtcagggcgt ggatgccggg gttgtggccg accaccatga
     1621 ccgtgtcgtg ttcgaccccg gcggcctcgg cctcgaacag gatgcgaccg gggtcggcat
     1681 gatacaggcc gcccagcggc cggaccgccg ccgcggggaa ggacagcgag acctgctccc
     1741 aggtctggcg ggtgcggcgg gcggcggaga ccaggccgac gtcgggaacc agcccctcgt
     1801 cgagcagcag gcggcccatc agggcggcgt cggcctggcc gcggtcggtc agggcgcgtt
     1861 cgaagtcgcc gccggggctg gagcgctccg ccttgccgtg gcgcatgagg atcaggcgtt
     1921 tcattgcgcc cctcataccc gccgccgtgg tgcgccgaaa ggcgccggtt gacagtcgcc
     1981 gccggcggcg gtccaaggcg cgatatgacc cttgaggcga gcagcgagat cgagcagcac
     2041 ggcggcgtct ggcggttccc agccgaccag cccctgctgc tcgattccgg cgggcggatc
     2101 gaggggctgg aggtcgcctg gaccagctac ggccggctca acgccgaccg gtccaacgcc
     2161 gtcctgatct gccacgccct gaccctggac cagcatgtcg ccgccaccca tccggtgacc
     2221 ggcaagcccg gctggtggac ccgtctggtc gggcccgggc ggccgctcga tccggcgcgc
     2281 cactacatcc tgtgcgccaa cgtcgtcggc ggctgcatgg gcacgaccgg accgggctcg
     2341 atcaacccgg tcaccgggcg accctggggc ctgaccttcc cggtggtgac catcgccgac
     2401 atggtccggg cccaggccat gctggtcgag gcgctgggaa tcgacacgct tctggccgtg
     2461 gtcgggggat cgatgggcgg catgcaggtg ctgcaatggg cggccagcta tcccgagcgg
     2521 atgttcagcg ccgtctgcgt cgcggccgcc gcccggcaca gcgcccagaa catcgccctg
     2581 cacgaggtcg gccggcaggc gatcatggcc gatccggact ggcgcggcgg cgactacgcc
     2641 ctgcacggga cccggcccga gaaggggctg gccgtcgccc gcatggccgc gcacatcacc
     2701 tatctgtccg aagcggccct gcagcggaag ttcggccgcg aactgcagcg cgacgggctg
     2761 agctggggtt tcgacgccga tttccaggtc gagagctacc ttcggcacca gggcgcgtcc
     2821 ttcgtcgacc ggttcgacgc caacagctat ctctacatca cccgggcgat ggactatttc
     2881 gacctcgcgg ccgcgcacgg cggggtgctg gccgaggcgt tccgcggcgc ccggcatgtc
     2941 cgcttctgca tcctgtcgtt cacctccgac tggctctatc cgacggcgga aagccggcac
     3001 atcgtgcgcg cgctcaatgc cgccggcgcg cgggccagct ttgtcgagat cgagagcgac
     3061 aagggccatg acgccttcct gctcgacgaa ccggtgatgg actcggcgct cgccggtttc
     3121 ctcgacgcgg tcggaaccgc caggggcctg acatgaccgc cgtgcgcgag gacttccgcg
     3181 agatcctgcg gctggtccgc cccggcgccc gcgtgctcga tgtcggctgc ggcggcggtg
     3241 agctgctcga gatgctggcc cgcgacaagc aggtcgacgg ccgcggcgtc gagatcagca
     3301 cccagggcgt atccgcctgt ctggcgcggg gcctggcggt gatgcagggc gacgccgatc
     3361 gcgacctcga ccagtttccg gccggcgcct ttgactacgc cgtgctgtcc cagaccctgc
     3421 aggccacgcg caatccgcgc caggtgctca gcgagctgct tcggatcgcc gaccgggcgg
     3481 tggtcagctt ccccaacttc ggccactggc gggtgcgctg gtcgctgttg agccggggcc
     3541 ggatgcccga gacccggtcg ctgcccgagc cctggtggtc gacgcccaac atccacctgt
     3601 gcaccctggc cgacttcaac gccctggtgg ccgacctcga cgtgcgcatc gaggcctgcg
     3661 ccgccctgtc cgaaggccgg ccggcccggc cgatgaaccc gaccagcctg ctcgagaact
     3721 ggcgggccga ggccgcgctg ttcctgctgg cgcgccgtaa cgccgagccg gcggaaccga
     3781 tcgacgcgcc ccgcgatctc ttcggcggat gaccattcgc ctgatgacct acaacgtcca
     3841 ccgttgcgtg ggggtggacc gcaagctcaa tgtcgagcgg gtggccgagg tgatcgccgc
     3901 ctgccgcccg gacatcgtcg ccctgcagga gctggacgtg ggccgggccc gcacgcgcgg
     3961 cgtcgaccaa gcgcaccggc tggcggagct gttgcagatg cgcgcccact tcaacgccgc
     4021 cctgacggtc gaggaggagc gctacggcga cgccatcctg accgccctgc ccgagaggct
     4081 ggtcaaggtc ggccctctgc cgggcctgcc gcggctgcgc gggctggagc cgcgcggcgc
     4141 cctgtgggtg gcggtcgacc tggacgggcg ggagctgcag gtgatcaaca cccatctggg
     4201 cctggtgccg cgcgaacagc aggtccaggt cgccgagctg ctcggccgtc gctggatggg
     4261 ggccgagggc ttcgagagcc cggcggtgct gctgggcgac tttaacgcca cgccgttttc
     4321 ccagacctat cgcatgctga cggccgtgct gcgcgacgcc caggacggcg ggccgaaggc
     4381 ggccacggcg acctttccct cgggctttcc gatgctgcgc atcgaccatg tcttcgtgag
     4441 cagcgagatc agggtcaggc gcgtcgagtc gcccttcgac ccgcgggcgc gggcggcgtc
     4501 ggatcatctg ccgctggtgg tggagatcga ggtcccggcg gcgcgggcgg agcaatcagc
     4561 gacagctggt ccctgagacg gcggcggcgc gcccacggcc gccaggcgtc ggagggcgcc
     4621 accggatcgc cgaggctcca ggcgctgatg aaccgctcga acggacccag cggccgggtc
     4681 tcgaccggcc gcagccgtcg tggggcgccg tcgagcaggt cg
//
LOCUS       DAORNX010000216         4714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13516_length_4714_cov_7.138656, whole genome shotgun sequence.
ACCESSION   DAORNX010000216 DAORNX010000000
VERSION     DAORNX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13516_length_4714_cov_7.138656"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1138
                     /locus_tag="PLO65_10080"
     CDS             50..1138
                     /locus_tag="PLO65_10080"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD28634.1"
                     /translation="MALTPGDPAPHFRAPSPGNPAYGFNTVGGRFVALAFLPPVATPT
                     TAAALNAVRSRRRLFDDERLCFFGVISDPAAIARARDEVPGIRWFHDPGGEIARIYEI
                     EAPGWVIVDPSLRVLFTAPLDQSDAVLARLAALPAIDDHAGAPLHAPVLIAPRIFEPA
                     FCRVLIDAYEAVGGTPSGFMREVDGRTVVASDPAHKRRSDVTIEDEGLRQAARARINR
                     RLVPLIRQAFQYAPTRMERYLVARYDADGGGWFRPHRDNTTRGTAHRQFAVSINLDAE
                     AHEGGDLRFPEFGSRTYRPPTGGAVVFSCSLLHEATPVTAGRRYAFLPFLYDEAGAQL
                     REANAAFVDPSIGGYRADVEAGTDRDQS"
     gene            complement(1129..2040)
                     /locus_tag="PLO65_10085"
     CDS             complement(1129..2040)
                     /locus_tag="PLO65_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD28635.1"
                     /translation="MTSPRSPFAASEWAAIAAIVLIWGVNNAAAKLATDVLPPFLVGG
                     VRFAIALLVLIPFLRPPFPPLRQVGPILLLLGPLHFGLIYYGFSIVKNLSPLVVSLQL
                     WIPMTAFFAWRMLGETMPRAGLAGMVVAFLGVAWMSLDPHAAGDLPGILIGVLASAMW
                     ALGTVWVRRSPAVTPLKMQALTSLVGAPALLAASFLFEDHVVARATTASWMIWATVVW
                     AALASTVGASALLFWLVQRREPGRVTPWFLLTPLVSCGIGIGLMGDHLTPQLALGGGA
                     TLVGVALVALSERRAAAVAASAASAQL"
     gene            2160..2438
                     /locus_tag="PLO65_10090"
     CDS             2160..2438
                     /locus_tag="PLO65_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRD28636.1"
                     /translation="MKQVTISELRRNLAQYLDDAVATGEPLVIRRKHGRLVLRSEPAS
                     SEQVSRAERMKAYFAAGSDLTPETLTIEEARQLTLAYSLPSDLPPAAD"
     gene            2435..2809
                     /locus_tag="PLO65_10095"
     CDS             2435..2809
                     /locus_tag="PLO65_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRD28637.1"
                     /translation="MIHLDTHAAIWLAEANERRLSRTARRLIDRQPLAISPAVVLELE
                     VLSEIGRLQNPPDAILTRLYDQADVSLSVTTFADVVAAATTFAWTRDVFDRLIVANAI
                     ADGARLLTADQHILDKFRDAVW"
     gene            complement(2829..3209)
                     /locus_tag="PLO65_10100"
     CDS             complement(2829..3209)
                     /locus_tag="PLO65_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28638.1"
                     /translation="MLLFFISSHHLTLVRSLVATRNRSGRWDVQAIDEERPGLVGSGL
                     TATRLPPWSLSIEDSRRLDRLLAGRSLYAEPVRQGSGPDQLGVGATFVSIEITTPSAR
                     RTSMLAGKWAGRTGDIVRLLSPKD"
     gene            complement(3392..3871)
                     /gene="ssb"
                     /locus_tag="PLO65_10105"
     CDS             complement(3392..3871)
                     /gene="ssb"
                     /locus_tag="PLO65_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRD28639.1"
                     /translation="MAGSVNKVILVGNLGKDPEIRTLNSGDRVANLSLATSETWRDRT
                     SGERKERTEWHRVVIFNDNLVKVAEQYLRKGSTVYIEGALQTRKWTDQQGVEKYSTEV
                     VLQKFRGELTMLGGRGDGGGAMRDEGGDFGGYSSGDRAQPSGPKESFSADLDDEIPF"
     gene            complement(4039..4419)
                     /locus_tag="PLO65_10110"
     CDS             complement(4039..4419)
                     /locus_tag="PLO65_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28640.1"
                     /translation="MSRVLAIAALAAAVLLGGCQETVEAPLDRGVCYAIGSTPEGQIK
                     FNVVARNIPNIENCAAQLEGMRLRFLRLGGQTRELSGAYQGNFIFLRREGIFRSQRWE
                     GNQYLMLVRSGDGRLVIPGAVQTE"
     gene            complement(4437..>4714)
                     /locus_tag="PLO65_10115"
     CDS             complement(4437..>4714)
                     /locus_tag="PLO65_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28641.1"
                     /translation="TQTTPPPADGDACWRYAGGGWSKVGSMDLNSCVQTLYAGQCEQR
                     GGAAYGRWGEQTLRLVRDRVEISSDNRRFRTLVEQGPNCSIPPVGQN"
BASE COUNT          757 a         1670 c         1567 g          720 t
ORIGIN      
        1 cgcaggcccg ggccttgccc cgcctgccgg gccttgccat cgtcaggcca tggccctgac
       61 ccccggcgac cccgcgcctc atttccgcgc gcccagcccc ggcaatccgg cctacggctt
      121 caacaccgtg ggcggacggt tcgtcgccct cgccttcctg ccgccggtcg cgacgccgac
      181 gaccgcggcc gccctgaacg cggtgcgatc ccgccgccgg ctgttcgacg atgagcgact
      241 gtgcttcttc ggcgtgatca gcgacccggc cgccatcgcc cgggcgcggg acgaggtccc
      301 gggcattcgc tggtttcacg atcccggcgg ggagatcgcg cggatctacg agatcgaggc
      361 gccgggctgg gtgatcgtcg acccctcgct gagggtgctg ttcacggccc cgctcgacca
      421 gtccgacgcc gtgctcgcgc ggctggccgc cctgccggcc atcgacgacc atgccggtgc
      481 gccgctgcac gctccggtgc tgatcgcccc gcggatcttc gagccggcct tctgccgcgt
      541 gctgatcgac gcctacgagg ccgtcggcgg gactccatcg gggttcatgc gagaggtcga
      601 cggccgcacc gtcgtggcca gcgacccggc gcacaagcgc cggtccgacg tgacgatcga
      661 ggacgagggc ctgcgccagg ccgcgcgcgc gcgcatcaac cggcggctgg ttccgctgat
      721 ccgccaggcc ttccagtacg cgccgacgcg catggagcgc tatctggtgg cccgctacga
      781 cgccgacggc ggcggctggt ttcgcccgca tcgcgacaac accacccgcg gcaccgccca
      841 tcggcagttc gccgtttcga tcaacctcga cgcggaggcc catgagggtg gcgacctgcg
      901 ctttcccgag ttcggcagcc gcacctatcg ccccccgacc ggcggggcgg tggtgttttc
      961 ctgttcgctg ctgcacgagg ccacgccggt gaccgccggc cggcggtacg ccttcctccc
     1021 gttcctctat gacgaggccg gggcgcagct gcgcgaagcc aacgccgctt tcgtcgatcc
     1081 gtcgatcggc ggctaccgcg cggatgtcga ggccgggacc gatcgcgatc agagctgagc
     1141 ggacgcggcc gaagcggcca cggccgcggc gcgccgctcg gacagggcca ccagggccac
     1201 gccgacgagg gtcgctccgc cccccagcgc cagctggggc gtcagatggt cgcccatcag
     1261 accgatgccg atgccgcagg acaccagcgg cgtgagcagg aaccacggcg tcacccgacc
     1321 gggctcgcgc cgctggacca gccagaacag cagggccgac gcgccgaccg tcgaggccag
     1381 ggcggcccag accaccgttg cccagatcat ccagctggcc gtcgtcgcgc gcgccacgac
     1441 atggtcctcg aacaggaagg acgcggccag cagcgccggg gcgccgacca gcgaggtcag
     1501 ggcctgcatc ttcagcgggg tgacggcggg cgaccggcgc acccagaccg tgcccagggc
     1561 ccacatggcg ctggccagca cgccgatcag gatgcccggc aggtcgccgg cggcgtgggg
     1621 atcgagactc atccaggcca cgccgaggaa ggccaccacc atgcccgcca gccccgcccg
     1681 cggcatggtc tcgcccagca tccgccaggc gaagaaggcg gtcatgggga tccacagctg
     1741 cagcgacacc accagcgggc tgaggttctt cacgatcgag aagccgtagt agatcaggcc
     1801 gaagtgcagc ggccccagca gcagcaggat tggcccgacc tggcgcagcg gcgggaacgg
     1861 cggccgcaga aaggggatca gcaccagcag ggcgatggcg aaccgcacgc cgccgacgag
     1921 gaacggcggc agcacgtcgg tggccagctt ggccgcggcg ttgttgacgc cccagatcag
     1981 cacgatggcc gcgatggcgg cccattcgga tgcggcgaag ggagagcggg gcgaggtcac
     2041 cgcgccctcc tcgcacggaa agccgtcgcg atggaacctc ggtggcggtc acagggggtt
     2101 acggggtgtg agcgggaatc ggcattgaca cgtacaaatg gcgaggccat tttgtacaaa
     2161 tgaagcaggt cactatttcg gagcttcggc gcaatctcgc ccagtatctg gacgacgcgg
     2221 tcgcgacagg cgagccgctg gtcattcgcc gcaagcatgg gcgccttgtg ttgcggtccg
     2281 aaccagcgag cagcgagcag gtctcgcgcg ccgagcgcat gaaagcctat ttcgcggctg
     2341 gctcggacct gactcctgaa accctgacca tcgaggaggc acgtcaattg accctggcct
     2401 actccctgcc gtccgacctg cctccggcgg ccgattgatc catctcgaca cccacgccgc
     2461 catctggctg gcggaggcca atgagcggcg cctgtcgcgc acggcccgcc gtctcatcga
     2521 tcgacaaccg ctcgcgattt ccccggccgt ggtgctggaa ctggaggtcc tttcggagat
     2581 tggccgactg cagaacccac cggacgcgat cctgacccgg ctctacgatc aagcggacgt
     2641 ctcgctatcg gtgaccacct ttgctgatgt ggtggcggcg gcgacgacct tcgcctggac
     2701 ccgcgacgtg tttgatcgac tgatcgttgc caacgccata gccgatggcg cccgcttgct
     2761 gaccgccgac cagcacatcc tggacaaatt tcgggatgct gtctggtgag tggactctga
     2821 ggtcgacgtc agtccttggg gctgagtagt ctcacgatgt ctcccgttcg ccccgcccac
     2881 tttcccgcga gcattgatgt gcgcctggct gaaggcgtcg tgatctcaat cgaaacgaac
     2941 gtggcgccga cacccagttg gtcgggcccg ctgccctgcc gaacgggctc ggcgtaaagg
     3001 ctgcgaccag caagcagccg gtccagtcga cgcgagtctt caatcgacag gctccagggc
     3061 ggcagccggg ttgccgtgag gccagagccc accagaccag ggcgttcctc gtcgatggcc
     3121 tgaacatccc agcgcccact gcgattgcgc gtcgcaacga gactacgcac tagcgtcaga
     3181 tggtggcttg aaatgaagaa gagcagcacg cgggtttcgc caaccgggac cgtaagcccc
     3241 tggcgagccc aaatgctctc gtgttccggc agcgcgctcg gcactgccgt agtggcttct
     3301 cggccgccag gcgcggcggc cgctcctcct gacagaaaga ggagcagccc aacgactgcc
     3361 agagccgccc atctcgccat cattcgtagc tctagaacgg gatctcgtcg tccaggtcgg
     3421 cgctgaagct ctccttcggg ccgctgggct gggcgcggtc gccgctggaa tagccgccga
     3481 agtcaccacc ctcgtcgcgc atcgccccgc cgccgtcgcc gcggccgccg agcatggtca
     3541 gctcaccacg gaacttctgc agcaccacct cggtggagta tttctcgacg ccctgctggt
     3601 cggtccattt gcgcgtctgc agcgcgccct cgatgtaaac cgtcgagccc ttcctgaggt
     3661 actgctcggc gaccttcacc aggttgtcgt tgaagatcac cacccggtgc cattcggtgc
     3721 gctccttgcg ctcgccgctg gtccggtcgc gccaggtctc cgaggtcgcc agactgagat
     3781 tggccacccg gtcgcccgag ttgagcgtgc ggatctccgg atccttgccc agattgccga
     3841 ccagaatgac cttgttgacg ctgcccgcca tggaagtgct ccttcaatcc tcacgcccgg
     3901 cgaccaggac ccgcgccgga atctgacgcc caaactagag ggcgttaacc acttgcgaaa
     3961 cccatgggtt catactttgt tctcacgcgc aagcgcttat gcacagcaaa tcgccgccgc
     4021 ctgaaacgcg cgaggccgct attcggtctg gacggcgccg ggaatgacca ggcggccgtc
     4081 gccgctgcgc accagcatca ggtactggtt cccctcccag cgctgggagc ggaagatgcc
     4141 ctcgcggcgc aggaagatga agttgccctg gtaggcgccc gacagctcgc gggtctggcc
     4201 gccgaggcgc aggaagcgca gccgcatgcc ctcgagctgc gcagcgcagt tctcgatgtt
     4261 ggggatgttg cgggccacca cgttgaactt gatctgtccc tcgggggtcg agccgatcgc
     4321 gtagcagacg ccccggtcca gcggcgcctc gacggtttcc tgacagccgc ccagcaggac
     4381 ggcggcggcg agcgcggcga tggcgagaac cctggacatg acggcgatac cctcgactag
     4441 ttctggccga ccggcggaat ggagcagttc ggcccctgct cgaccagggt gcggaagcgg
     4501 cggttgtccg aagagatctc gacccggtca cggaccagac gcagggtctg ctcgccccac
     4561 cgaccgtagg ccgcgccgcc ccgctgttcg cactggccgg catagagcgt ctggacgcag
     4621 ctgttgagat ccatcgaacc gaccttcgac cagccgccgc cggcgtagcg ccagcaggcg
     4681 tcgccgtcgg cgggcggcgg cgtggtctgg gtcg
//
LOCUS       DAORNX010000217         4706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13551_length_4706_cov_6.947968, whole genome shotgun sequence.
ACCESSION   DAORNX010000217 DAORNX010000000
VERSION     DAORNX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13551_length_4706_cov_6.947968"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLO65_10120"
     CDS             complement(<1..259)
                     /locus_tag="PLO65_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HRD28642.1"
                     /translation="MPRDTNDTPQTPAGLVYTDSSDPGLVRCKTRGGFVYRDAAGRPV
                     RDEATLERIRRLAAPPAWSQVWICADPRGHIQAVGRDARGRK"
     gene            308..1135
                     /gene="rlmJ"
                     /locus_tag="PLO65_10125"
     CDS             308..1135
                     /gene="rlmJ"
                     /locus_tag="PLO65_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRD28643.1"
                     /translation="MNYRHAFHAGNFADLVKHATLTRLLDALAADPAPLTVIDTHAGA
                     GGYDLEGELARRTGEAAQGVFRLMSADDAPRAFERLRAAVRAGNPAGGVRFYPGSPSL
                     IAAALRKGDRYVACELRDDDVALLDQTLAPYPGAKAVQTDGFAMAEREAGKGGRLFVL
                     IDPPFERGDDYARIAATLTSVLLLDPDACVAVWLPLKDLETFDAFLRSVEPACDDLLV
                     AEARLKPLTDPMKMNGCAMVIANPPRDVEDSLAGICDWVVGRLGEPGGRAEVWRTGR"
     gene            1212..1784
                     /locus_tag="PLO65_10130"
     CDS             1212..1784
                     /locus_tag="PLO65_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28644.1"
                     /translation="MDKPAFPTVETEKMLRLPVGAISPLWLMFAGAATVGAAWYWSRT
                     FFRPTNLEALFEAPEAAAETTEDLAEALVEAGVELARTEIEAVEAALETVVEIVDEAV
                     EAAPEPVAEVLAALAPEPVALPESDDLTVLTGIGPKLAAALAERGVTRFAQIAAWSEA
                     DTARFDKDLKLMGRIGREAWIAQARRLAEG"
     gene            complement(1781..2761)
                     /gene="meaB"
                     /locus_tag="PLO65_10135"
     CDS             complement(1781..2761)
                     /gene="meaB"
                     /locus_tag="PLO65_10135"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRD28645.1"
                     /translation="MSSTPDIASLQRGLLAGDRAALARAITLVESRRADHQAAARALL
                     EQVMPHTGGSQRIGVTGVPGAGKSTTIEALGCNLTAAGHRVAVLAVDPSSSRTGGSIL
                     GDKTRMERLSVDPDAFIRPSPSGGTLGGVARKTREAMLLCEAAGFDVVIVETVGVGQS
                     ETVVADMVDMFVALLIPGGGDELQGIKKGLIEIADLIVINKADADPQRAERSAREYRA
                     ALHILTPASPDWTPPVITASGLNNEGLDRLWAQIERHREVMGANGERATRRAAQNARW
                     MWAMVRDRLEEAFRDHPDVAAQAHDLERQVRAGRLPASAAADRLLKAFGL"
     gene            complement(2771..3088)
                     /locus_tag="PLO65_10140"
     CDS             complement(2771..3088)
                     /locus_tag="PLO65_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRD28646.1"
                     /translation="MLITTTPFIEGRPVKEYKGAIYAQSILGANVVVDLLGAIRDFIG
                     GHSRSYERVLARAREDAMKNLEKEAEKLGANCIIAVDLDYNTVGPQAQMLMVSVSGTA
                     VVI"
     gene            3287..3763
                     /locus_tag="PLO65_10145"
     CDS             3287..3763
                     /locus_tag="PLO65_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRD28647.1"
                     /translation="MFLARSHLDLHNIKNSIDRTKKMSDGIVKIGPFGFGLDALLGFV
                     PWAGGLYSGGAGFVLCIDAVRARAAPMVVAEMALILAVDTLAPFIPKAGGVIDALFTG
                     HKWAADLLLRHMDETIYFEGSREQAQGSSEYRDLMARVRAGKEKRRVVFLGKSPKA"
     gene            3814..4434
                     /locus_tag="PLO65_10150"
     CDS             3814..4434
                     /locus_tag="PLO65_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRD28648.1"
                     /translation="MSRDRAHSAWKSAESVKKLSDRVIGIGPFGVGLDGILTWIPGAG
                     IVYSAGASAYLLYLAMSSGASLGTLAKMAGYLGLDMLVSDFPIPGVSDLFDLLWQGHL
                     LAANSLQKDIEARHGVPADRIAAVEKARRRRGRGLGWIVLLLLVALGYVVWRSDFNET
                     LHLGWWPAFKAASLPLVGYSVSLPLAAGVLLALIFVTNIVNRTRRA"
     gene            4495..>4706
                     /locus_tag="PLO65_10155"
     CDS             4495..>4706
                     /locus_tag="PLO65_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28649.1"
                     /translation="MSDKQPYVRPPSAESLGAAPGRDRLTGRRILVVGGGQQTLDAAT
                     DPVGNGRAMARLFAREGATVAVADRSR"
BASE COUNT          703 a         1627 c         1637 g          739 t
ORIGIN      
        1 gcttgcggcc tcgggcgtcg cgaccgacgg cctggatgtg cccgcgcggg tcggcgcaga
       61 tccacacctg ggaccaggcg ggcggcgccg cgaggcggcg gatccgctcc agcgtggcct
      121 catcccgcac cggccgcccg gccgcgtcgc gatagacgaa accgccgcgg gtcttgcatc
      181 gcaccaggcc ggggtcgctg ctgtcggtat agaccaggcc ggcaggcgtc tgcggcgtat
      241 cgttcgtgtc gcgcggcatg gctcgccaac gtggcgggaa acgccggagt tccgctacag
      301 aagcgagatg aactatcgcc acgcctttca cgccggaaac ttcgccgatc tcgtcaagca
      361 cgcgaccttg acgcggctgc tcgacgccct ggccgccgac cccgcgcccc tgaccgtcat
      421 cgacacccat gccggcgcgg gcggctatga cctcgaaggc gagctggccc gtcggacagg
      481 ggaggcggcg cagggggttt tccgcctgat gtcagccgat gacgcacccc gggcgttcga
      541 acgcctcagg gcggcggtcc gcgcgggcaa tccggccggc ggcgtgcggt tctatccggg
      601 atcgccaagc ctgatagcgg ccgccctgcg caagggcgac cgctatgtcg cctgcgaact
      661 gcgcgacgac gacgtcgccc tgctggatca gacactcgcg ccatatccgg gcgcgaaggc
      721 cgtgcagacc gacggcttcg ccatggccga gcgggaggcg ggcaagggcg ggcggctgtt
      781 cgtcctgatc gacccgccgt tcgagcgtgg cgacgactac gcacggatcg ccgcgaccct
      841 gacctcggtg ctgctgctcg atccggacgc ctgtgtcgcg gtctggcttc ccctcaagga
      901 tctcgagacc ttcgacgcct tcctgcggtc agtggagccg gcctgcgacg acctgctcgt
      961 cgccgaggcc cggctcaagc cgttgaccga tccgatgaag atgaacggct gcgcgatggt
     1021 catcgccaat ccgccgcgag acgtcgagga ctccctggcc ggcatctgcg actgggtggt
     1081 cggcagactc ggcgagcccg gtggccgggc ggaggtctgg cgcacggggc ggtaacaaac
     1141 atttcgcact tgcgaaacgg aattccatga tatgttgcgc tgcacaattg gccgcgccac
     1201 ggagatcgct catggacaag cccgccttcc cgaccgtcga aaccgaaaag atgttgcgcc
     1261 tcccggtcgg cgcgatctcg ccgttgtggc tgatgttcgc cggcgcggcg accgtcgggg
     1321 ccgcctggta ctggagccgg accttcttca ggccgaccaa tctcgaggcc ctgttcgagg
     1381 cgccggaagc cgccgccgaa accactgaag acctggccga agcgctggtc gaggccgggg
     1441 tcgaactggc ccggaccgag atcgaagccg tcgaggcggc gctggaaacg gtcgtcgaga
     1501 ttgtcgacga agccgtcgaa gccgcgcctg agccggtggc cgaggtgctc gcggccctcg
     1561 cgcccgagcc ggtcgcgctg cccgaatccg acgacctgac ggtgttgacc ggcatcggcc
     1621 ccaagctggc cgccgccctg gccgaacggg gcgtgacccg cttcgcccag atcgcggcct
     1681 ggagcgaggc cgataccgcc cggttcgaca aggacctgaa gctgatgggc cggatcggcc
     1741 gcgaagcctg gatcgcgcag gcgcgtcgcc tcgccgaagg ctagagcccg aacgccttga
     1801 gcagccggtc ggcggcggcg gaggccggca ggcggccggc gcgcacctga cgctcgaggt
     1861 cgtgcgcctg ggcggccaca tccggatggt cgcggaaggc ttcttccagc cgatcccgga
     1921 ccatcgccca catccagcgg gcgttctgcg cggcccgtcg cgtcgcccgt tcgccgttgg
     1981 ctcccatgac ctcgcggtgg cgctcgatct gggcccagag cctgtcgagg ccctcattgt
     2041 tcaggcccga ggcggtgatc accggcggtg tccagtcggg cgaggccggg gtcaggatgt
     2101 gcagcgccgc gcggtattcc cgcgccgacc gttcggcccg ctgcggatcg gcgtcggcct
     2161 tgttgatcac gatcaggtcg gcgatctcga tcagtccctt cttgatcccc tgcagttcgt
     2221 ctccaccgcc cgggatcagc agggcgacga acatgtcgac catgtccgcg accaccgtct
     2281 ccgactggcc gacccctacg gtctcgacga tcaccacgtc gaagccggcg gcctcgcaca
     2341 gcagcatggc ctcgcgggtc ttgcgggcga cgccgccgag cgtcccgccg gagggcgagg
     2401 ggcggatgaa ggcgtcggga tcgaccgaca gccgctccat gcgcgtcttg tcgccgagga
     2461 tcgatccacc cgtgcgactg gatgagggat ccaccgccag caccgccacc cgatggccgg
     2521 ccgcggtcag gttgcagccg agggcctcga tggtggtcga cttgccggcg ccgggcacgc
     2581 cggtgacccc gatgcgctgg gacccgccgg tatgcggcat gacctgctcg agcaacgccc
     2641 tggcggcggc ctgatggtcc gcgcggcgac tctcgaccag ggtgatggcg cgcgccagcg
     2701 cggcgcggtc accggccagc aggccgcgtt ggagcgaagc gatgtccggg gtcgagctca
     2761 cgggcccggt ctagatgacg acggccgttc ccgaaaccga aaccatcagc atctgcgcct
     2821 gtggcccgac ggtgttgtag tccaggtcga cggcgatgat gcagttggcg ccgagcttct
     2881 ccgcttcctt ctcgagattc ttcatcgcgt cctcgcgggc gcgcgccaga acccgctcgt
     2941 aggagcgcga atgcccgccg atgaaatcac ggatggcgcc cagcaggtcg acaacgacgt
     3001 tcgcgcccag aatcgactgg gcgtagatcg cgcccttgta ttccttcaca ggccgaccct
     3061 cgatgaacgg ggtggtcgtg atcagcatct gcgactcccg gcaaacgacc cccgactatt
     3121 aaggctaaac gcccggaacg gcgagggttc tcctgacagg ggaaaatgga gcccaaaccg
     3181 gtgcgccgtt gcgcgaaaag cacgccgccg cccttcctga gcgaacacgc ttattgcaga
     3241 gtggccccct gcgacggcgt tccctgtgcc gtcgggagac gccgtgatgt tcctcgcccg
     3301 atcgcacctc gatctgcaca acatcaagaa cagcatcgac cggacgaaga agatgtccga
     3361 cggcatcgtc aagatcggcc ccttcggctt cggtctggat gcgttgctcg gcttcgtgcc
     3421 ctgggccggc ggcctctaca gcggcggggc cggcttcgtg ctgtgcatcg acgccgttcg
     3481 cgcgagggcc gcgccgatgg tcgtggccga gatggccctg atcctggccg tcgacaccct
     3541 ggccccgttc attcccaagg ccggtggcgt catcgacgcc ctgttcaccg gtcacaaatg
     3601 ggccgccgac ctgctgctgc gccacatgga cgagacgatc tatttcgagg gcagccgcga
     3661 gcaggcccag ggctcgtcgg aataccgcga cctgatggcc cgggtccgcg ccggcaagga
     3721 gaagcgtcgc gtggtgttcc tcggcaagtc accgaaggcc tgaccggatt tccactggcg
     3781 gaaccttggt cggccgactt aggttagcgt ctcatgagca gggaccgcgc ccacagcgcc
     3841 tggaagtcgg ccgagtcggt caagaaactg tccgaccggg tgatcggtat cggcccgttc
     3901 ggcgtcggcc tcgacggcat cctcacctgg atcccgggcg caggcatcgt ctacagcgcc
     3961 ggggcgtccg cctacctgct gtacctggcg atgagttcgg gggcctccct gggcaccctg
     4021 gccaagatgg cgggctacct gggcctggac atgctggtgt cggatttccc gatcccgggg
     4081 gtatcggacc tgttcgacct gctctggcag ggccatctgc tggccgccaa ttccctgcag
     4141 aaggacatcg aggcgcgcca cggcgtgccg gccgaccgga tcgccgccgt cgagaaggcg
     4201 cgccgcaggc gcggccgggg tctcggctgg atcgtgctgc tgctgctggt cgcgctgggc
     4261 tacgtggtct ggcgttcgga cttcaacgag accctgcatc tgggctggtg gccggcattc
     4321 aaggcggcca gccttccgct ggtcggctat tcggtgagcc tgccgctggc cgcgggcgtg
     4381 ctgctggcct tgatcttcgt gaccaacatc gtcaaccgga cccgccgcgc ctgaccgccg
     4441 gacggtcctg cggatgcggc ctggtccagg cccagataga accggagtcg ttgcgtgagc
     4501 gacaagcaac cctatgtgcg gccgcccagc gcggagtccc tcggcgcggc gccggggcgg
     4561 gaccggctga ccggtcgacg gatcctggtc gtgggcggcg ggcagcagac cctcgacgcc
     4621 gcgaccgacc cggtcggcaa cggccgggcc atggcgaggc tgttcgcgcg cgagggtgcg
     4681 actgtcgcgg tcgccgatcg cagccg
//
LOCUS       DAORNX010000218         4702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13572_length_4702_cov_8.772972, whole genome shotgun sequence.
ACCESSION   DAORNX010000218 DAORNX010000000
VERSION     DAORNX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13572_length_4702_cov_8.772972"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLO65_10160"
     CDS             <1..619
                     /locus_tag="PLO65_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRD28650.1"
                     /translation="AEVGAAIAGEVPWVKSWRTTGPSLDGPLADFEGLTPDLRAAFDA
                     DMARAARDGFCFRFDAWRLSDDILAGKRRGGVLAPLEAVHDFLNGEAFLGFVRTLTGE
                     PRCAYCDVMVSRYRAGDFLSAHDDDIVGKKRLFAYVLNFTPVWRADWGGVLLFLDEDS
                     HVAEGYTPTFNALNLFSVPQAHAVSYVTPFAAGPRLSITGWVRAR"
     gene            619..1542
                     /locus_tag="PLO65_10165"
     CDS             619..1542
                     /locus_tag="PLO65_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD28651.1"
                     /translation="MPEEPPPVSPLAERPRLAPNVRGALWMLASALGFTVMTTLIKFL
                     GADYPAALQTFYRQAAGLLVLAPLILRDWRGAFHTTRPGILFFRSAVGTLAMIFSFYA
                     FQKLPLAEANALSFTRTLWMVPLAIFVLREKTGPLRIGAAMVGFVGVLVMLRPGASDH
                     GFGLGQLASLASAAMFAMTITGMKVMTRDHSPFTLLVWSAVLGLVFSVPPAMFVWRWP
                     EPFDLLLLAAMGVFGTITQACYIKGMQIGDAGAMAPIDYSRLVFTTATGFFIFHEIPG
                     WATIIGAAIVVASTLFITFREQQQARQAQKG"
     gene            complement(1530..2186)
                     /locus_tag="PLO65_10170"
     CDS             complement(1530..2186)
                     /locus_tag="PLO65_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRD28652.1"
                     /translation="MEQTSSVLMAVSGLAALGYGLGMAGGAPSLQRTVVKSAAVGALA
                     VLAFLSDAPWLLAAALLLCALGDAFLAGDPRRWLPAGMAAFLLGHILLIFLFQETRDP
                     ALEMTPAQLVGAVGVGLAAVAMLAWLWRSLGPMRAAVTLYVIAIAVMVGSSFLLPAFY
                     WPAMVGAVAFMASDAILAGDLFRGAKLAASPRLTGLAIWFLYYGGQALIAWAFLRQPF
                     "
     gene            complement(2207..2611)
                     /locus_tag="PLO65_10175"
     CDS             complement(2207..2611)
                     /locus_tag="PLO65_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRD28653.1"
                     /translation="MSAESKPSPDVNPAIDPARALTPAVIKVNERRVAQGFWPKMRRV
                     AARIPFASEALSVWWCARDPETPAAAKGLMLAALAYFVLPTDAIPDILAGIGFTDDAA
                     VFAALMAVVGKNIKPRHREAAQAWLRKMTADD"
     gene            2665..3453
                     /locus_tag="PLO65_10180"
     CDS             2665..3453
                     /locus_tag="PLO65_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28654.1"
                     /translation="MKLDSSISAVVTGGASGLGEATVRALVAQGVKVAIFDLDRQADR
                     GAALEKELGVTFCAVNVTSDADVDAGFVKARAANGQERILVNCAGTGNAAKTASRDKN
                     TGEIKHFPLAHFDMIIQINLVGTFRCIAKSAAGMLTLDPLPDGDRGAIVNTASVAAED
                     GQIGQAAYSASKGGVVGMTLPIARDLSSEGIRVNTILPGIFNTPLLNAAPEAVKAALG
                     AQVPFPKRLGQAEEYASLALEMIRNGYFNGEDVRLDGAIRMAPR"
     gene            complement(3561..4136)
                     /locus_tag="PLO65_10185"
     CDS             complement(3561..4136)
                     /locus_tag="PLO65_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28655.1"
                     /translation="MTFKAAAIAAALVATAGLSACATATPYQPNIRGQAVSGGFSETR
                     LEADRFRVTFAGNSLTSRETVERYLLYRAAELTVAQGADWFSLVERQTDRKARTYVDP
                     DPYGRFGPYGHWRPYWSYYGPRWGWRAWDPWYGDPFWADRIDVRTVERFEATAEIVVG
                     RGPRPANDPRAFDAREVLANLGPTIVRPTPG"
     gene            complement(4219..>4702)
                     /locus_tag="PLO65_10190"
     CDS             complement(4219..>4702)
                     /locus_tag="PLO65_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD28656.1"
                     /translation="NAATLVWFAGAALGLSALMAAFPAAFRLLAIAGGLYVAWLGISS
                     IRSGLRGGGGPVDRPGGGPVRSAFLDGFSVQISNPKALLFFTAVLPPFLDLSRPLAGQ
                     LPMYAAATIGMDVIAMSAYALGGAALAGRMTEPRFRRAFAFVVGGLLVAVSILILSRR
                     "
BASE COUNT          732 a         1688 c         1611 g          671 t
ORIGIN      
        1 ggccgaggtc ggggcggcga tcgccggcga ggtgccctgg gtcaagagct ggcgcaccac
       61 cggtccgagc ctcgacggcc cgctggccga cttcgagggc ctgacgccgg acctgcgggc
      121 ggccttcgac gccgacatgg cccgggcggc gagggacggc ttctgcttcc gcttcgacgc
      181 ctggcgactg agcgacgaca tcctcgccgg caagcgccgc ggcggggtgc tggcgccgct
      241 cgaggcggtc catgacttcc tcaacggcga ggccttcttg ggattcgtcc ggaccctgac
      301 cggcgagccg cgctgcgcct attgcgacgt gatggtctcg cgctaccggg ccggggactt
      361 cctgtcggcc catgacgacg acatcgtcgg caagaagcgg ctgttcgcct atgtgctgaa
      421 cttcaccccg gtgtggcgcg ccgactgggg cggggtgctg ctgttcctcg acgaggacag
      481 ccatgtcgcc gagggctaca ccccgacctt caacgccctg aacctgttca gcgtgcccca
      541 ggcccatgcg gtgtcctatg tgaccccgtt tgcggccggg ccgcggctgt cgatcaccgg
      601 ctgggtccgg gcccgctgat gcccgaggaa ccaccccccg tttcgccgct ggccgagagg
      661 ccgaggctgg cccccaacgt ccggggcgcc ctgtggatgc tggcctcggc gctcggcttc
      721 acggtgatga ccaccctgat caagttcctc ggcgccgact atccggccgc gctgcagacc
      781 ttttatcgcc aggccgccgg gctgctggtg ctggcgccgc tgatcctgcg cgactggcgc
      841 ggcgccttcc acacgacgcg gccgggtatc ctgttcttcc gctcggcggt cggcaccctg
      901 gcgatgatct tcagcttcta cgccttccag aagctgccgc tggcggaggc caacgccctg
      961 tcgttcaccc gcaccctgtg gatggtgccg ttggcgatct tcgtgctgcg ggaaaagacc
     1021 ggtccgctgc gcatcggcgc ggcgatggtc ggcttcgtcg gcgtgctggt gatgctgcgg
     1081 cccggcgcgt cagaccacgg cttcggcctg ggacagctgg cctcgctggc ttcggcggcg
     1141 atgttcgcca tgaccatcac cggcatgaag gtgatgaccc gtgaccactc gccgttcacc
     1201 ctgctggtct ggtcggcggt gctgggcctg gtcttctcgg tcccgccggc gatgttcgtc
     1261 tggcgctggc ccgaaccgtt cgacctgctg ctgctggcgg ccatgggcgt gttcggcacc
     1321 atcacccagg cctgctacat caagggcatg cagatcggcg acgccggagc catggcgccg
     1381 atcgactaca gccggctggt gttcaccacc gccaccggct tcttcatctt ccacgagatc
     1441 ccgggctggg cgacgatcat cggcgcggcg atcgtcgtcg catcgaccct gttcatcacc
     1501 ttccgcgaac agcagcaggc ccgccaggct cagaagggct gacgcaggaa ggcccaggcg
     1561 atcagggcct ggccgccgta atacaggaac cagatcgcca ggccggtcag gcgaggcgag
     1621 gcggccagtt tggcgccgcg aaacaggtcg ccggcgagga tagcgtccga ggccatgaag
     1681 gccaccgccc cgaccatcgc cggccagtaa aaggccggca gcaggaaact tgacccgacc
     1741 atcaccgcga tggcgatgac atagagggtc accgccgccc gcatcggtcc aagcgatcgc
     1801 cacagccagg ccagcatggc caccgcggcc agacccacgc ccacggcgcc gaccagctgc
     1861 gccggcgtca tctcgagcgc cgggtcgcgc gtctcctgga acaggaagat cagcaggatg
     1921 tggccgagca gaaaggccgc catcccggcc ggcagccatc gccgggggtc gcccgccagg
     1981 aaggcgtcgc cgagcgcgca gagcagcagg gccgccgcca gcagccaggg cgcgtccgac
     2041 aggaaggcca gcaccgccag ggcgccgacg gcggcgctct tgaccaccgt ccgctgcagc
     2101 gacggcgcgc cgccggccat gcccaggccg tagcccaggg cggcgagccc cgagaccgcc
     2161 atcaggacgc tgctggtctg ttccatggcc cccgtcagcc gctgcgtcag tcgtccgccg
     2221 tcattttccg cagccaggcc tgggcggcct cgcggtgacg cggcttgatg ttcttgccga
     2281 ccaccgccat cagggcggcg aacacggcgg cgtcatcggt gaacccgatc ccggcgagga
     2341 tatcgggaat cgcgtcggtc ggcaggacga aataggccag cgccgccagc atcagcccct
     2401 tggcggccgc cggcgtctcg ggatcgcggg cgcaccacca gacggacagg gcctccgagg
     2461 cgaaggggat gcgcgcggcg acacgccgca tcttgggcca gaagccctgt gcgacgcgac
     2521 gttcgttgac cttgatcacg gccggcgtca gcgcccgcgc cggatcgatg gccgggttga
     2581 cgtcgggtga cggcttggat tcggcgctca tcgggctaaa cccctcttcg aactgtattc
     2641 aacacaagga tcggggagcc gtccatgaag cttgattcct ccatttccgc cgtcgtcacc
     2701 ggcggtgcgt ccggcctcgg cgaggccact gtccgcgccc tggtcgccca gggcgtcaag
     2761 gtcgccatct tcgacctcga tcgccaggcc gatcgcggcg ccgcgctcga gaaggagctg
     2821 ggcgtgacct tctgcgccgt caacgtaacc agcgacgccg acgtcgacgc cggcttcgtc
     2881 aaggcccgcg cggccaatgg ccaggagcgt atcctggtca actgcgccgg caccggcaac
     2941 gccgccaaga ccgccagccg cgacaagaac accggcgaaa tcaagcactt cccgctggcc
     3001 cacttcgaca tgatcatcca gatcaacctg gtcgggacct tccgctgcat cgccaagtcg
     3061 gcggccggca tgctgaccct ggacccgctg ccggacggcg atcgcggcgc catcgtcaac
     3121 accgcctcgg tggcggccga ggacggccag atcggccagg cggcctattc ggcctccaag
     3181 ggcggcgtcg tcggcatgac cctgccgatc gcccgcgacc tgtccagcga aggcatccgc
     3241 gtcaacacca tcctgccggg catcttcaac accccgctgc tgaacgccgc gccggaagcg
     3301 gtcaaggccg ccctgggcgc ccaggtgccc ttccccaagc ggctcggcca ggctgaagag
     3361 tacgcctcgc tggctctgga aatgatccgc aacggctact tcaacggcga ggacgtccgt
     3421 ctggacggcg cgatccggat ggcgccgcgg taggactccg cgtggttcga cacgcgatcc
     3481 tccggatcgc tgctcaccat gacgtgtctt cgggcccgtc aacctgagca ggccgcccgg
     3541 cggccgtgtc gaagaacgca ctatcccggc gtcggccgca cgatggtcgg accgaggttg
     3601 gccagcacct cccgcgcgtc gaacgcccgt gggtcgtttg cgggcctggg cccacggccg
     3661 accacgatct cggccgtggc ctcgaatcgc tcgaccgtgc gcacgtcgat ccggtccgcc
     3721 cagaacggat cgccatacca gggatcccag gcgcgccagc cccagcgggg gccgtagtag
     3781 ctccagtagg gccgccaatg gccataggga ccgaaccggc cataagggtc gggatcgacg
     3841 taggtgcgcg ccttgcggtc ggtctggcgc tcgaccagcg agaaccagtc cgcgccctgg
     3901 gcgacggtca gttcggcggc gcgatagagc agatagcgct cgaccgtttc gcgcgaggtc
     3961 aggctgttgc cggcgaaggt cacccggaag cgatccgcct ccagccgggt ctccgaaaag
     4021 ccgccggaca ccgcctggcc gcggatattg ggttggtagg gggttgccgt ggcgcaggcc
     4081 gaaaggcccg ccgtcgcgac cagcgccgcc gcgatcgcgg ccgccttgaa ggtcatcgtc
     4141 gttcgctcca gcacagatcc atcgtctgcc cacaacacat ggcgcccccg accggttgcg
     4201 ccaagggggc gcgccccgtc agcggcggct caggatgagg atcgaaacgg cgacgagcag
     4261 gccgccgacg acgaaggcga aggcccggcg gaagcgcggc tcggtcatcc ggcccgccag
     4321 ggccgcgccg cccagggcat aggcggacat ggcgatcaca tccatgccga tggtggcggc
     4381 ggcgtacatc ggcagctgcc cggccagcgg gcgcgagagg tcgaggaacg gcggcaggac
     4441 cgcggtgaag aacagcagcg ccttgggatt ggagatctgc accgagaagc cgtcgaggaa
     4501 ggccgaccgc acgggcccgc cgccgggccg gtcgaccggt ccgccgccgc ccctgagccc
     4561 cgaccggatc gagctgatgc cgagccaggc gacgtacagc ccgccggcga tggccagcag
     4621 ccggaaggcg gccggaaacg ccgccatcag cgcgctgagg cccagggcgg cgccggcgaa
     4681 ccacaccagg gtcgccgcgt tc
//
LOCUS       DAORNX010000219         4688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13619_length_4688_cov_7.876970, whole genome shotgun sequence.
ACCESSION   DAORNX010000219 DAORNX010000000
VERSION     DAORNX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13619_length_4688_cov_7.876970"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..355
                     /locus_tag="PLO65_10195"
     CDS             101..355
                     /locus_tag="PLO65_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HRD28657.1"
                     /translation="MAPDRVETYAPGMAHRVVGYSEEEAVISDTRYLKEIDEGGPVNG
                     PAPDLALHYRGLAPTAGLPQTTMAACPPHVALEPNRKAAA"
     gene            359..781
                     /locus_tag="PLO65_10200"
     CDS             359..781
                     /locus_tag="PLO65_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRD28658.1"
                     /translation="MEPRPRIGPGVAVGVTEAMIADLVHAFYGRVRQDPVLGPIFDRA
                     IGDGWDEHLARLCDFWSSVLLMTGRFKGAPMAAHARLPEIEAPHFRRWLALFRETAGQ
                     GCPPEAAALFVEKAETIARSLQMGLAVSRGELPPVRAE"
     gene            complement(904..2313)
                     /gene="glnA"
                     /locus_tag="PLO65_10205"
     CDS             complement(904..2313)
                     /gene="glnA"
                     /locus_tag="PLO65_10205"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRD28659.1"
                     /translation="MAKAKDILAMIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDF
                     LEDGTMFDGSSIAGWKAINESDMKLRPDLDTAYIDPFYQQTTLCLFCDVVNPDTGTPY
                     NRDPRSMAKTALTYVKASGIGDTVFFGPEAEFFIFDDVRWSTAPHDTGYSFDSIELPV
                     NTSKAYPEGNMGHRPGPKGGYFPVNPVDSAQDLRGEMLAVMGELGMKPEKHHHEVAPA
                     QHELGLKFDTMVTMADRLQLYKYVIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIW
                     GDGKPLFAGDQYAGLSQMCLWYIGGIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLA
                     YSSRNRSASIRIPHVDSPKAKRIECRFPDPMGNPYLTFTALLMAGLDGIENRIDPGAP
                     ADKNLYDLPPREQKKIPEVCGSLREALENLDKDRAFLKKGGVMDDDFIDSYIELKMEE
                     VMRLQLHPHPVEFDMYYKC"
     gene            complement(2362..2700)
                     /locus_tag="PLO65_10210"
     CDS             complement(2362..2700)
                     /locus_tag="PLO65_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD28660.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQDLGVQGMTVLEAKGYGRQKGHTE
                     LYRGAEYVVDFLPKIKVEVVVSDDQLAGALEAIQNAARTGRIGDGKIFVSEISDVIRI
                     RTGEAGSAAI"
     gene            2919..4463
                     /locus_tag="PLO65_10215"
     CDS             2919..4463
                     /locus_tag="PLO65_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRD28661.1"
                     /translation="MRDDTPVIIGAGQFTYRGPADTSPSPLDLLKIAAERAVADAGLE
                     AGVLAQLDGVAVVGFTIDAPGALSQLPFPRLKNPPASLARAYGADPRYAIYSHMGGNS
                     PQQLVNTLCERIARGENELTIAIGAEFLGSLMKRLKGGAGFAGWEDPFAIEDEPARIG
                     DPRPGTTPQENAHGLNRPINVYPMFENALRARDGRSIEDHQKRLGRLFAPFSQVASTN
                     PHAWFPTERSESELITVTDQNRMISFPYPKYLNAIMEVDQSAGVIIASVRKARELGVP
                     ESKWVFLHGCADASDLWYPSDRQDFHSSPAMRLTGKRAFEMARIGPDDLDYIDLYSCF
                     PIAVEVGAEELGLSLDDPRGLTVTGGLPYMGGPGNNYAMHSIATMIGKLRDRPGSWGL
                     CTANGWYLTKQSTGIYSTTPVEGMWEREDPAVIQRQIDALPHPPLVEHPEGPATIEAY
                     TVVHTRDGYHMGIVFGRDSHDRRFIAVTPDDEATLRDLEAREGVGRTGVVGRHPDGKR
                     NLFTPD"
     gene            4554..>4688
                     /locus_tag="PLO65_10220"
     CDS             4554..>4688
                     /locus_tag="PLO65_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD28662.1"
                     /translation="MRTYMIRHGKPSATWGDQDDDPGLDETGQAQARAAAAILLALPE
                     H"
BASE COUNT          752 a         1558 c         1590 g          788 t
ORIGIN      
        1 gccgccgggg tcagcgtcgc ggccgaaggg gcgtgcgggc cggcctgagg ccgcgccggg
       61 gacgcccgac cccgggaaaa cccgtagggg gaaaccccta atggccccgg accgggtaga
      121 gacctatgcc ccgggcatgg cgcaccgcgt cgtcggctat agcgaggaag aggcggtgat
      181 cagcgacacc cgctatctca aggaaatcga cgagggcggg ccggtgaacg gtcccgcgcc
      241 ggatctggcc ctccactacc ggggcctggc cccgacggcc ggcttgccgc agaccaccat
      301 ggctgcctgt ccgccgcacg tggcgctcga acccaaccgg aaggccgcgg cctgaccgat
      361 ggagccacgt ccccgtatcg gacccggcgt cgcggtcggc gtcaccgagg cgatgatcgc
      421 cgacctggtg cacgccttct acggccgggt tcgccaggac cctgtgctgg gtccgatttt
      481 cgaccgggcg atcggcgacg gctgggatga gcacctggcc aggctgtgtg acttctggtc
      541 gtcggtgctg ctgatgaccg gccggttcaa gggcgcgccc atggccgccc atgcccggct
      601 gccggaaatc gaggcgccgc atttccgccg atggctggcc ctgtttcgcg agacggccgg
      661 ccaaggctgc ccgcccgagg cggcggcgct gttcgtcgag aaggcggaga ccatcgcccg
      721 cagcctgcag atgggcctgg ccgtcagccg cggcgagctg ccccccgtcc gggccgaatg
      781 acaaaagggc cggagatcgc tctccagccc ttccgggggg accatgtacc gtaggtacgt
      841 gtccccgata tgtgcgcccc cgggtttcgg ggacacgtac cttcggtaca tgtcccctct
      901 acgctagcac ttgtaataca tgtcgaattc gaccgggtgc gggtgcagct gcagccgcat
      961 cacctcttcc atcttcagct cgatgtagct gtcgatgaag tcgtcatcca tgacgccgcc
     1021 cttcttgagg aaggcgcggt ccttgtcgag gttctcgagc gcctcacgca ggctgccgca
     1081 gacttccggg atcttcttct gctcgcgggg cggcaggtcg tagaggttct tgtcggccgg
     1141 cgcgcccggg tcgatgcggt tctcgatccc gtccagcccc gccatcagca gggcggtgaa
     1201 ggtcaggtag gggttgccca tgggatcggg gaagcggcac tcgatacgct tggccttggg
     1261 gctgtcgacg tgcgggatgc ggatcgaggc cgagcggttg cggctggagt aggccagctt
     1321 caccggcgcc tcgtagcccg gcaccaggcg cttgtagctg ttggtcgtcg agttggagaa
     1381 ggcgttgatc gccttggcat gcttgatgat gccgccgatg taccacaggc acatctgcga
     1441 caggccggcg tactggtcgc cggcgaacag cggcttgccg tcgccccaga tcgactggtg
     1501 aacgtgcatg cccgagccgt tgtcgccgaa catcggcttg gccatgaagg tggcggtctt
     1561 gccgtaggcg tgtgccacgt tgtggatcac gtacttatac agctgcagcc ggtcggccat
     1621 ggtcaccatg gtgtcgaact tcaggcccag ttcgtgctgc gccggggcga cctcgtggtg
     1681 gtgcttctcg ggcttcatgc ccagctcgcc catcaccgcc agcatctcgc cgcgcaggtc
     1741 ctgggcgctg tccaccgggt tgaccgggaa gtagccgccc ttggggccgg gccggtggcc
     1801 catgttgcct tcgggatagg ccttggaggt gttcaccggc agttcgatcg agtcgaagct
     1861 gtagccggtg tcgtgcgggg ccgtggacca gcgcacgtca tcgaagatga agaactccgc
     1921 ttccgggccg aagaacaccg tgtcgccgat gcccgaggcc ttcacatagg tgagggcggt
     1981 cttggccatc gagcgcgggt cgcgattgta gggcgtgccc gtgtccgggt tcaccacgtc
     2041 gcagaacagg cacagggtcg tctgctggta gaacgggtcg atgtaggcgg tgtcgaggtc
     2101 cggacgcagc ttcatgtcgg actcgttgat cgccttccag ccggcgatcg acgaaccatc
     2161 gaacatcgtg ccgtcctcga ggaaatcctc atcgaccaga tcaatgtcga aggtgacgtg
     2221 ctgcatcttg ccgcgaatgt cggtgaagcg gacgtcgacg tatttgacgt ccttctcctt
     2281 gatcatcgcc agaatgtctt tggccttggc catgtgccct atcccctgag ctgatggtgc
     2341 ggaacttgtg gttggttgtc gctagatggc ggctgatccg gcttcgccgg tgcggatgcg
     2401 gatgacgtcg gagatctccg agacgaaaat cttgccgtcg ccgatgcggc cggtgcgggc
     2461 ggcgttctgg atcgcctcca gggcgccggc cagctggtca tcggacacca ccacctcgac
     2521 cttgatcttg ggcaggaagt cgacgacgta ctcggcgccg cgatagagct cggtatggcc
     2581 cttctggcgg ccgtagccct tggcctccag cacggtcatg ccctggacgc cgaggtcctg
     2641 cagggcctcc ttcacctcat cgagcttgaa cggcttgatg atcgcttcga tcttcttcat
     2701 gcgggcctct gtggcgcggt tggcgcgacg gagcacgggg ccttgcagcg gttcaaggcc
     2761 cgtcgctccc gacgcgcgtt gcagcgcagc gtaaaggatg gggcgcccat caaacaggcg
     2821 cccggcgccc aagacaaggg cagggcgccc aaaggccttg gttgccgccg gggcagggcg
     2881 ctaccgaggc ggaaatgcag gcggaaggga acggaacgat gcgtgacgac acaccggtga
     2941 tcatcggagc gggtcaattc acctatcgcg gtccggccga cacctcgccg tcgccgctcg
     3001 atctgctcaa gatcgcggcg gaacgggcgg tcgccgacgc ggggctggag gccggcgtgc
     3061 tggcgcagct cgacggcgtg gcggtggtcg gcttcaccat cgacgctccc ggcgccttgt
     3121 cgcagttgcc gtttccgcgc ctgaagaacc ccccggccag cctcgcccgg gcctatggcg
     3181 ccgatccgcg ctacgccatc tactcccaca tgggcggcaa cagtccccag cagctggtca
     3241 acaccctctg cgagcggatc gcccgcggcg agaacgagct gaccatcgcc atcggcgcgg
     3301 agttcctcgg ttcattgatg aagcggctca agggcggggc cggcttcgcc ggctgggagg
     3361 accccttcgc catcgaggac gagcccgcca ggatcggcga tccccggccc ggcacgacgc
     3421 cgcaggagaa cgcccacggc ctgaaccggc cgatcaacgt ctacccgatg ttcgagaacg
     3481 ccctgcgggc ccgcgacggc cggtcgatcg aagatcacca gaagcgcctg ggcaggttgt
     3541 tcgcgccgtt cagccaggtc gcctcgacca atccccacgc ctggttcccg accgagcgca
     3601 gcgaatcgga gctgatcacg gtcaccgacc agaaccgcat gatcagcttt ccctatccga
     3661 agtacctcaa cgccatcatg gaggtcgacc agtcggccgg ggtgatcatc gccagcgtcc
     3721 gcaaggcgcg cgagctgggc gttcccgaga gcaagtgggt gttcctgcat ggctgcgccg
     3781 acgccagcga cctgtggtac ccgtcggacc gccaggactt ccattccagc ccggccatgc
     3841 gcctgaccgg caaacgggcc ttcgagatgg cccgcatcgg ccctgacgac ctcgactaca
     3901 tcgacctcta ttcttgcttc ccgatcgcgg tcgaggtggg cgccgaggaa ctgggcctgt
     3961 cgctggacga cccgcgcggc ctgaccgtga ccggcgggct gccgtacatg ggcgggccgg
     4021 gcaacaacta cgccatgcac tcgatcgcca cgatgatcgg caagctgcgc gaccgcccgg
     4081 gcagctgggg cctgtgcacg gccaacggct ggtacctgac caagcagtcg accggcatct
     4141 attcgaccac gccggtcgaa gggatgtggg agcgcgagga tccggcggtc atccagcgcc
     4201 agatcgacgc cctgccgcat ccgccgctgg tcgagcaccc ggaaggcccg gcgacgatcg
     4261 aggcctacac ggtggtccac acccgcgacg gctatcacat gggtatcgtc ttcggccgcg
     4321 acagccacga ccgccgcttc atcgcggtca cgccggacga cgaggcgacc ctcagggatc
     4381 ttgaggcccg cgagggcgtc ggccggaccg gcgtggtcgg gcgtcatccg gacgggaagc
     4441 gcaacctgtt cacgccggat tgacgagcag ggacatgcac ttcagtgcgt gtcccttgca
     4501 gcgagacccg gggacacgca ctgaagtgca tgtccccctc ggaagaggcc agaatgcgca
     4561 cctacatgat ccgccacggc aagccttcgg ccacctgggg cgaccaggac gacgatcctg
     4621 gcctcgacga gaccggccag gcccaggcgc gggccgccgc ggcgatcctg ctggccctgc
     4681 cagagcat
//
LOCUS       DAORNX010000220         4675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13667_length_4675_cov_7.645671, whole genome shotgun sequence.
ACCESSION   DAORNX010000220 DAORNX010000000
VERSION     DAORNX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13667_length_4675_cov_7.645671"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLO65_10225"
     CDS             <1..910
                     /locus_tag="PLO65_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD28663.1"
                     /translation="ELLRRAELAVEAAKSAGRGGGAAYGRAMESDGLSQLALESDLRG
                     AVARGEVVPFFQPVVRLSDGALSGFEALCRWRHPRRGLIPPDDFLPMAEELGMMAELG
                     AHMLRASARQLAAWRQSHPAAGNLTVSVNLSTGEIHREGLVSDVTGLIAKHRLPPGSL
                     KLEITESDIMRDPEGAAAVLRALRGGGAGLALDDFGTGFSSLSYLTRLPFDTLKIDRY
                     FVRTMGANEGSAKIVRSVIKLGQDLNLEVVAEGVENAIMARQLLDLGCDYGQGFGYAP
                     ALSPQEAEVYLNESYVDGAAPVRRQG"
     gene            complement(1024..1602)
                     /locus_tag="PLO65_10230"
     CDS             complement(1024..1602)
                     /locus_tag="PLO65_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRD28664.1"
                     /translation="MTEPSEADFLSGRLLIAMPGIGDPRFERAVVFLCAHDAEHAIGL
                     AVNRPVDGLTLESLFERIGVDPPVRRVADEPVLMGGPVERERGFVLHTDDYLLDGISL
                     PVTRGLALTTTRDVLEAMGGEAAPRRAILALGYAGWGPGQLEREVRENVWLVCDPDEA
                     LIFGDDHDHKWSRALEKLGISAAMLSEGGGRA"
     gene            1713..2195
                     /locus_tag="PLO65_10235"
     CDS             1713..2195
                     /locus_tag="PLO65_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD28665.1"
                     /translation="MTIKAGDKLPDATFMTSGPEGPRPITTDEIFKGKTVAVLALPGA
                     FTPTCSAKHLPGFKEHAAEFRAKGVDTIACISVNDVFVMKAWGADQGVGDDILMLADG
                     NGDFTKAIGLEMDGSKFGMGLRSQRYSMIVKDGVVDTLNVEAGGEFKVSSADYMLAQL
                     "
     gene            complement(2293..3966)
                     /locus_tag="PLO65_10240"
     CDS             complement(2293..3966)
                     /locus_tag="PLO65_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD28666.1"
                     /translation="MTYRPPLRDMTAALKAAGFDRLGDAFPDADAETAWAVLEAAGEF
                     AAGVIAPLNRQGDLSGATYANGRVTAAPGFADAYRQFAAGGWNSLSAEADFGGQGLPK
                     TLEIAVFEMIQAASLAFGLCPMLTQGAIEALAAHGTDSQKARFLPRLVSGEWTGTMNL
                     TEPQAGSDLAALTTRAEPDGEGGWRLTGQKIFITWGEHDVADNIVHLVLARLPDAPEG
                     SRGISLFVCPKRLLNADGTAGAPNAVRPVGVEHKLGIHGSPTCVMAFEGAKAELVGDP
                     HQGLAAMFTMMNAARLQVGAQGVGIAERAWQQALAFSLERRQGRSAWTGENNAPIHDH
                     PDVRRMLMLMRAKILAARAICLSTAVAADLSRHAATEDERTTAKLREELLTPIAKAWS
                     TDLGVEAASLGVQIHGGMGFIEETGAAQHYRDARILPIYEGTNGIQAIDLVGRKLTLG
                     GGAALASLLADIRETAAGEPRLAAGADALEEAATWLSARKGTPDALAGATGFLKLAGD
                     VTGGWMLAKMAASDAAYAGVSRFYGEQILATVPGQVAGVVQGAAVLEVE"
     gene            4093..>4675
                     /locus_tag="PLO65_10245"
     CDS             4093..>4675
                     /locus_tag="PLO65_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRD28667.1"
                     /translation="MFRPFSLALALVLMTAPMALAAPSTDPADMPAGTYAVDKTHASL
                     IARVRHMGLADYTVRFTGFDASYDWDPKSPQTARVEATFQAASLDTGEPKINGEFAEK
                     FLDAKANPTVRFVSTSIVQTSPGKGTMTGDLTLRGVTKPVTLDVVFNGYVSGMAGQRS
                     GFSARGVIKRSEFGSTFLLNPPLAFVGDEVELIL"
BASE COUNT          687 a         1675 c         1586 g          727 t
ORIGIN      
        1 cgaactgctg cgccgggcgg agctggcggt cgaggcggcc aagagcgcgg gtcgcggcgg
       61 cggcgcggcc tatggccggg cgatggagag cgatggcctg tcgcagctgg ccctggaatc
      121 ggacctgcgc ggcgcggtcg cccgcggcga ggtggtgccc ttcttccagc ccgtcgtgcg
      181 gctgtccgac ggggccctgt cgggcttcga ggcgctgtgt cgctggcgtc atcctcgccg
      241 cggcctgatc ccgcccgacg atttcctgcc gatggccgag gaactgggca tgatggccga
      301 gctgggcgcg cacatgctgc gcgcctcggc ccggcagttg gccgcctggc gtcagagcca
      361 cccggccgcc ggcaacctga cggtcagcgt caacctgtcg accggcgaga tccatcgcga
      421 ggggctggtg tccgacgtca ccggcctgat cgccaagcat cgtctgcccc ccggctcgct
      481 gaagctggag atcaccgaga gcgacatcat gcgcgacccg gagggcgcgg ccgcggtgtt
      541 gcgggcgctg cgtggcggcg gcgccggcct ggcgctggac gacttcggca ccggcttctc
      601 gtcgctcagc tacctgaccc gcctgccgtt cgacacgctg aagatcgacc gctacttcgt
      661 gcgcaccatg ggcgccaacg agggctcggc caagatcgtc cggtcggtga tcaagctcgg
      721 ccaggacctc aacctcgagg tcgtcgccga gggcgtcgag aacgccatca tggcccgcca
      781 gctgctcgac ctgggctgcg actatggcca gggcttcggc tacgccccgg ccctctcgcc
      841 gcaggaggcg gaggtctatc tcaacgagag ctacgtcgac ggggcggcgc cggtgcgccg
      901 gcaggggtag ctcccttcct ctccgcggag cggagagggg gaccatgcgg agcatggtgg
      961 agaggcggcg ccgaggcccg ccggaacgat cgtcgctgcg ctccggggag gatcgcgccg
     1021 tcgctatgcc cgaccgccac cctccgacag catcgccgcc gagatcccca gcttctccag
     1081 ggcccgcgac catttgtggt cgtggtcgtc gccgaagatc agggcctcgt cgggatcaca
     1141 gaccagccag acgttctcgc gcacctcacg ctccagctgg cccgggcccc agccggcata
     1201 gccgagcgcc aggatggccc ggcgcggagc cgcctcaccg cccatggctt caagcacgtc
     1261 gcgggtcgtg gtcagggcca ggccccgggt caccggcagg ctgattccgt cgagcagata
     1321 gtcgtcggta tgcagcacga agccgcgctc gcgttccacc ggcccgccca tcagcaccgg
     1381 ctcgtcggcg acgcggcgca ccggcgggtc gacaccgatt cgctcgaaca gcgattccag
     1441 ggtcaggcca tccaccggac gattgaccgc cagcccgatc gcatgttcgg cgtcatgggc
     1501 gcagagaaac accacggccc gttcgaaccg ggggtcgccg atgcccggca tggcgatcag
     1561 gagacggccg gacaggaagt ctgcttccga aggctcggtc atgcgttgat gatcggcatt
     1621 gatggcgccg gattcaagtc gcccgcttgg cgaaggggtc cgtcgcctct atttgcacca
     1681 gcaaccgcgg ttccgcgaca gggagagacc caatgacgat caaagccggt gacaagttgc
     1741 ccgacgccac cttcatgacc tcgggacccg aggggccccg tccgatcacc accgacgaga
     1801 tcttcaaggg caagaccgtc gccgtcctgg ccctgccggg cgccttcacc ccgacctgct
     1861 cggccaagca tctgcccggc ttcaaggagc acgccgccga attccgcgcc aagggcgtcg
     1921 acaccatcgc ctgcatctcg gtcaacgacg tgttcgtgat gaaggcctgg ggcgcggacc
     1981 agggcgtggg cgacgacatc ctcatgctgg ccgacggcaa cggcgacttc accaaggcca
     2041 tcggcctgga gatggacggc agcaagttcg gcatgggcct gcgctcgcag cgctactcga
     2101 tgatcgtcaa ggacggcgtg gtcgacacgc tgaacgtcga ggccggcggc gaattcaagg
     2161 tctcgtcggc cgactacatg ctggcgcagc tgtagggttc tgatcgggga catgtgccga
     2221 aggcacgtgt ccccccaacc cctgtgacag ctctattggg ggacacggac cttcggtacg
     2281 tgtccccttc tgctattcca cttccagcac cgcagcgccc tggaccaccc cggccacctg
     2341 gcccgggacc gtggccagaa tctgctcgcc gtagaagcgc gagactccgg cgtaggccgc
     2401 gtcggacgcc gccatcttcg ccagcatcca accgcccgtc acatcgcccg ccagtttcag
     2461 gaaccccgtc gcgcccgcca gcgcgtcggg cgtgcccttg cgggccgaca gccaggtggc
     2521 ggcttcttcc agagcatccg caccggccgc caggcggggc tcgcccgccg cggtctcgcg
     2581 gatgtcggcc agcaatgagg ccagcgccgc gccgccgccg agggtcagtt tgcggccgac
     2641 caggtcgatc gcctggatgc cattggtccc ctcgtagatc ggcaggatgc gggcgtcgcg
     2701 atagtgctgc gccgcgccgg tctcctcgat gaagcccatg ccgccatgaa tctgcacccc
     2761 caggctggcg gcctcgacgc cgaggtcggt cgaccaggcc ttggcgatcg gagtcagcag
     2821 ttcctcacgc agcttggccg ttgtgcgttc gtcctcggtc gccgcgtggc gcgacagatc
     2881 cgcggcgacc gccgtcgaca ggcagatggc ccgggcggcg aggatcttcg cccgcatcag
     2941 catcagcatg cggcggacgt ccggatggtc atggatcggg gcgttgttct cgccggtcca
     3001 ggccgaccgc ccctgtcgcc gctccagcga gaaggccaac gcctgctgcc aggcccgctc
     3061 ggcgatgccg acgccctggg cgccaacctg cagccgggcg gcgttcatca tggtgaacat
     3121 cgccgccagg ccctggtgcg ggtcgccgac cagctcggcc ttcgccccct cgaaggccat
     3181 cacgcaggtc ggtgagccgt ggatgcccag cttgtgttcg acgccgactg gccgcaccgc
     3241 gttgggcgcg ccggccgttc cgtcggcgtt cagcagtcgc ttggggcaga cgaacagcga
     3301 gatgccgcgg ctgccctccg gcgcgtcggg caggcgggcg agcaccagat ggacgatgtt
     3361 gtcggcgacg tcgtgctcgc cccaggtgat gaagatcttc tgcccggtca ggcgccagcc
     3421 gccctcgccg tccggctcgg cccgggtggt cagggcggcc aggtccgagc cggcctgcgg
     3481 ctcggtcagg ttcatcgtgc cggtccattc gccgctgacc agccggggca ggaaacgcgc
     3541 cttctggctg tccgtaccgt gggcggccag cgcctcgatc gccccctggg tcagcatcgg
     3601 gcacagaccg aaggccaggc tcgcggcctg gatcatttcg aagaccgcga tctcgagcgt
     3661 cttgggaagg ccctgaccgc cgaagtcggc ctccgcggac aggctgttcc agccgcccgc
     3721 ggcgaactgg cgataggcgt cggcgaagcc gggcgccgcg gtcacccgcc cgttggcgta
     3781 ggtcgcgccg ctcaggtcgc cctggcggtt cagcggcgcg atcacccccg cggcgaactc
     3841 gcccgcagcc tcgagcacgg cccaggccgt ctcggcgtcg gcgtccggga aggcatcgcc
     3901 cagccggtcg aagccggccg ccttcagcgc ggcggtcatg tcgcggagtg gcggacgata
     3961 ggtcatggag cgtctccctg tgccggagcg ttgtaggacg ccggtcgccg tttcgtgaag
     4021 cctcggcctt tcctccgccc cactccggac ctatgtagtt ctgcaacaca accggtcaca
     4081 atcgaggtcg ccatgttccg cccgttctca ctcgccctgg cccttgtcct gatgaccgcc
     4141 ccgatggcgc tggcggcgcc ctccacggac cccgccgaca tgcccgccgg gacctatgcg
     4201 gtggacaaga cccacgccag cctgatcgcc cgtgtccggc atatgggcct ggcggactac
     4261 acggtccgtt tcaccggctt tgacgcgtct tacgactggg acccgaagtc gccgcagacg
     4321 gcccgggtcg aggcgacctt ccaggccgcc tccctcgaca ccggcgagcc gaagatcaat
     4381 ggggagttcg ccgagaagtt tctcgacgcc aaggccaacc cgaccgtccg cttcgtttcc
     4441 acctcgatcg tccagaccag cccgggcaag gggaccatga ccggcgacct gacccttcgc
     4501 ggcgtgacga aaccggtgac gctcgacgtg gtcttcaacg gctacgtctc gggcatggcc
     4561 ggccagcgct cgggcttttc ggcgcggggt gtgatcaagc ggtccgagtt cggatcgacc
     4621 ttcctgctca acccgccgct ggcctttgtc ggcgacgagg tcgagttgat cctcg
//
LOCUS       DAORNX010000221         4674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13673_length_4674_cov_7.552284, whole genome shotgun sequence.
ACCESSION   DAORNX010000221 DAORNX010000000
VERSION     DAORNX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_4674_cov_7.552284"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLO65_10250"
     CDS             complement(<1..488)
                     /locus_tag="PLO65_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD28668.1"
                     /translation="MAVASEGGLVERRFMREAMRAPLLDVDREQALARRWRDEADDAA
                     LQELTGAYMRLVIAMAGKFRRYGLPMADLVQEGAVGLMQAADRFDPDRGVRFSTYAGW
                     WVRAAMQDYVLRNWSIVRTGTTASAKSLFFKLRGLRARLGDLEGRLTAEARAGIAAAL
                     GV"
     gene            630..1070
                     /locus_tag="PLO65_10255"
     CDS             630..1070
                     /locus_tag="PLO65_10255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD28669.1"
                     /translation="MKQIRTEAVISAPPAKVWAVLTDFDRYHEWNPLNIAAQGEARAG
                     GKVRTTFLNPAGKPGATVTQTVRLTTFEPYTALAWTGVVPLLFHGTHLFLLEPAPEGT
                     KLIHGENMSGLIAMTFSAEQLERDFRPHYVAVNEALAKRVAAVG"
     gene            complement(1098..1532)
                     /locus_tag="PLO65_10260"
     CDS             complement(1098..1532)
                     /locus_tag="PLO65_10260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRD28670.1"
                     /translation="MKPPAWRQDRALYPHSVRVQTRYHDEDRLGHVNNIAVAAYYDEA
                     RSQLMRRTFALTNGLTGVRIVTADVRVSYMGEVFHPGDVEVASGILRIGNSSWEIGQT
                     MFQNDRCVGVATTVLVQASEAGAGTIVPELRAALEQLMVVQP"
     gene            complement(1606..2202)
                     /locus_tag="PLO65_10265"
     CDS             complement(1606..2202)
                     /locus_tag="PLO65_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRD28671.1"
                     /translation="MTLPASPSSARNREPILAQLRRWLPAAGTVLEVASGLGEHAAWF
                     AEALPGITWQPTDRSEDALEILRARREAAGLANLAPPRVLNAAEPDSWPLDRADAVVC
                     INMVHISPWAATQGLMAGAARVLPVGGPLILYGPYIEAGVDTAPSNLAFDADLKRRDP
                     AWGLRDLAAVTALAAEHGLTLAGRVAMPANNLCLLLRR"
     gene            2288..2893
                     /locus_tag="PLO65_10270"
     CDS             2288..2893
                     /locus_tag="PLO65_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRD28672.1"
                     /translation="MPRQARTVIPALPHHVVQRGNRRQPTFFRPADYASYLTIAGDQF
                     RAAGVEVLAYCLMPNHVHLIATPRDEMGLAIAVGRTHLKYTRYINARETWTGCLWQGR
                     FQSSPMDDAYFRRCLRYVGLNPVRAGLVERAADWPWSSVQAHLGVRADRLITPGAVNA
                     VLDEEGDGFFEVDLRGDDLARLRADLREGRPLAEAGQRPRD"
     gene            complement(2999..3502)
                     /locus_tag="PLO65_10275"
     CDS             complement(2999..3502)
                     /locus_tag="PLO65_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRD28673.1"
                     /translation="MSKSGLAFSVDDHVVYPAHGVGTIRAVETQEVAGMSLEVYVITF
                     DHEKMTLRVPTKKAVTSGLRPLAEGNVVSKALTTLKGRARVKRTMWSRRAQEYEAKIN
                     SGDLISIAEVVRDLHRAENQPEQSYSERQLYESALDRMAREVAAIEKIDRDAAVGILT
                     KSLQKAA"
     gene            complement(3807..4148)
                     /locus_tag="PLO65_10280"
     CDS             complement(3807..4148)
                     /locus_tag="PLO65_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRD28674.1"
                     /translation="MTYIVTDACIKCKFMDCVEVCPVDCFYEGENFLVISPDECIDCG
                     VCEPECPVDAIKPDTEDEPDGKWLKINSEYARVWPNITLKGEPPADAEAFERETGKFE
                     KYFSEKPGKGS"
     gene            complement(4297..4581)
                     /locus_tag="PLO65_10285"
     CDS             complement(4297..4581)
                     /locus_tag="PLO65_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRD28675.1"
                     /translation="MSDESCRADVWLWRARFFKTRSMAARFVDEGRVRFTRAGVAGRL
                     DKASRALKRGDSLVFAVGGRVIAVRLEAFGERRGPPAEARALYAVIEAGP"
BASE COUNT          745 a         1593 c         1590 g          746 t
ORIGIN      
        1 tcgacgccga gggcggcggc gatgccggcc cgcgcctcgg cggtcagtcg cccctcgagg
       61 tcgcccagcc gggcgcgcag gccgcgcagc ttgaagaaca gactcttggc cgaggcggtg
      121 gtcccggtgc gcacgatcga ccagttgcgc agcacatagt cctgcatcgc cgcccgcacc
      181 caccatccgg cataggtgga aaaccgcacc ccgcggtcgg ggtcgaaccg gtcggccgcc
      241 tgcatcagcc cgaccgcgcc ctcctgcacc aggtcggcca tcggcaggcc atagcggcgg
      301 aacttgccgg ccatggcgat gaccagccgc atataggcgc cggtcagctc ctgcagggcc
      361 gcgtcgtcgg cctcgtcgcg ccagcgccgc gccagggcct gctcgcggtc cacgtccagc
      421 aatggcgcgc gcatcgcctc ccgcatgaag cgccgctcga ccagtccgcc ttccgatgcg
      481 accgccatgg cctgtctcct ttgaacggcg atacgcgggc agggtaaccg tggatcacct
      541 gccgccgtgg agaaggaggc atgctcccga cgcgcgcgcg ccgcggcgga cggttgatcc
      601 cgccgccggg ctccggcagg ctcgccgcca tgaaacagat ccgcaccgag gccgtcatct
      661 ccgcgccgcc ggcgaaggtc tgggccgtgc tgaccgactt cgaccgctat cacgagtgga
      721 acccgctcaa catcgcggcg cagggcgagg cccgggccgg cggcaaggtc cgcacgacct
      781 tcctcaatcc ggcgggcaaa cccggcgcga cggtgaccca gacggtgcgc ctgaccacct
      841 ttgagcccta cacggccctg gcctggaccg gcgtcgtgcc gctgctgttc cacggcacgc
      901 acctgttcct gctggagccc gcgcccgagg ggacgaagct gattcacggc gagaacatga
      961 gcggcctgat cgccatgacc ttctcagccg agcagctcga gcgcgacttc cggccccatt
     1021 atgtggcggt caacgaggcg ctggccaaac gggtggcggc ggtcgggtag gggacatgta
     1081 ccgacggtac gggtctccta cggctgaacc accatcagct gctccagcgc ggcgcgcagt
     1141 tcggggacga tcgtccccgc gccggcctcc gaggcctgga ccagcaccgt ggtggcgacc
     1201 ccgacgcagc ggtcgttctg gaacatggtc tggccgatct cccacgaaga attgccgatc
     1261 cgcaggatgc cggaggcgac ctccacatcg ccggggtgga acacctcgcc catgtagctg
     1321 acccgcacgt cggcggtgac gatccgcacc ccggtcaggc cgttcgtcag cgcgaaggtg
     1381 cgccgcatca gctgcgagcg cgcctcgtcg taataggccg ccacggcgat gttgttgacg
     1441 tgccccagcc ggtcctcgtc gtgatagcgg gtctgcaccc ggacgctgtg cggatagagg
     1501 gcgcggtcct ggcgccaggc gggtggtttc atcggcgggg actcgggtct gtgtgatgct
     1561 tcgccaagac tggacgggcg ccgttggggc agtccactgg cagcgttacc gccgcagcag
     1621 caggcagagg ttgttggccg gcatggcgac ccgcccggcg agggtcaggc cgtgttcggc
     1681 ggccagcgcc gtgacggcgg ccaggtcccg caggccccag gcgggatcac ggcgcttgag
     1741 gtccgcgtcg aaggcgaggt tgctcggcgc ggtgtcgacc ccggcctcga tgtacgggcc
     1801 atagaggatc agcggcccac cgacgggcag cacccgcgcc gccccggcca tcagcccctg
     1861 cgtcgccgcc cagggcgaga tgtggaccat gttgatgcag acgaccgcgt cggcccggtc
     1921 gagcggccag ctgtccggct cggcggcgtt gaggacccgc ggcggggcga ggttggccag
     1981 gccagccgcc tcgcgccgcg ctctcaggat ctccagcgcg tcctcagacc ggtcggtcgg
     2041 ctgccaggtg atccccggca aggcctcggc gaaccaggcg gcgtgctcgc ccagtccgct
     2101 ggccacctcg agcaccgtcc cggcggccgg cagccagcgg cgcagctgcg cgaggatcgg
     2161 ctcgcgattg cgggcggagg acggggaggc gggcagggtc atcggggaca tgtacctgca
     2221 aggtacgtgt cccccaatca aaatatctga gagattgtaa attgagatat gcacgttatg
     2281 gtaagtgatg ccgcgtcagg cccgcaccgt cattcccgcg ttacctcacc acgtggtgca
     2341 gcgtgggaac cgtcgccaac ccaccttctt ccggccggct gactacgcgt cctacctgac
     2401 gattgccggt gaccagttca gagcggcggg tgttgaagtc ctggcctact gtctgatgcc
     2461 caaccacgtg catctgatcg ccacgcctcg ggacgagatg ggcctggcaa tcgccgtcgg
     2521 ccggacgcac ctcaaataca cccggtatat caacgcgagg gagacttgga ccgggtgtct
     2581 ctggcaaggg cgcttccagt cgtcacccat ggatgacgcc tacttccggc gctgcctgag
     2641 atacgttggc ctcaatccgg ttcgcgcggg tctcgtcgag agggcggcgg actggccctg
     2701 gtcgagtgtt caggcgcatc tcggcgtcag ggcggatcgc ctgattaccc cgggcgcggt
     2761 caacgcggtg ttggatgagg agggcgacgg gttctttgaa gtcgacctgc ggggggatga
     2821 tctcgctcgc ctccgagccg accttcggga ggggagacca cttgccgaag caggccaaag
     2881 gccgcgggat taggggacac gtaccttcgg tacatgtccc cgacatcacg ccccgcgcaa
     2941 agcaaaacgc ccggcgtcgc cgccgggcgt ctcacgaaat cacagcacct gagaacgcct
     3001 aggcggcttt ctgcagggac ttggtcagga tgccgacggc ggcgtcgcgg tcgatctttt
     3061 cgatcgcggc gacttcgcgg gccatgcggt ccagcgccga ttcatagagc tgccgctccg
     3121 aataggactg ttccggctgg ttctcggcgc ggtgcaggtc gcgcaccacc tcggcgatcg
     3181 agatcaggtc gccggaattg atcttggctt cgtattcctg ggcgcggcgc gaccacatgg
     3241 tgcgcttgac ccgggcgcgg cccttgaggg tcgtcagcgc cttggacacc acattgccct
     3301 cggcgagcgg acgcaggccc gaggtgacgg ccttcttggt cggcacccgc agggtcatct
     3361 tctcgtggtc gaaggtgatg acatagacct cgagcgacat gccggcgact tcctgggtct
     3421 cgaccgcgcg gatcgtgccc accccgtggg cggggtagac aacatggtcg tcgaccgaaa
     3481 acgccagacc agacttgctc atatgttcgt ccttctcttg caccctgtcc gcgggggtcc
     3541 tccccgcggg ccgcagcccg gagacagcca gaacgagacg aaaactcctc cgaccgcggc
     3601 gtaaaccgcg gggggatttc gtttcagtgt ggacagacct ctaggacacc ctcgccttca
     3661 atcaggcgcg ccgttcactt gcggctgttc ttgaaccaag gcgcgacggc ggacccgtca
     3721 cgtcagacag gaccctatca caaaattgag cctaaagaaa gagttgcgaa tacagtcggc
     3781 gcccgtcggc cttccgctcc ggcgtctcag gagcccttgc cgggcttctc gctgaagtac
     3841 ttctcgaact tgccggtctc gcgttcgaac gcctcggcgt ccgccggcgg ttcgcccttg
     3901 agcgtgatgt tgggccacac ccgggcgtat tcggaattga tcttcagcca cttgccgtcg
     3961 ggctcgtcct cggtgtccgg cttgatcgcg tcgaccgggc attcgggttc gcagacgccg
     4021 cagtcgatgc attcgtcggg gctgatcacc aggaagttct cgccctcgta gaagcagtcg
     4081 accgggcaga cctccacgca gtccatgaac ttacatttga tgcaggcgtc ggtgacgata
     4141 taggtcatcg gtaatcttgg cggtccagac ggttgggtgc gggcgccttt tcaaaggcgg
     4201 gggccggctt gtcaatgcgg cgaggtctct cagtggccgc aggaaaactc aagatggcgc
     4261 ggcagatttg atctggcgca gatcgccggg accgggctac ggccccgcct cgatcacggc
     4321 atagagcgcc cgggcctcgg cggggggacc gcgccgctct ccaaacgcct ccaggcgaac
     4381 cgcgatcacc cggccgccca cggcgaagac cagggagtcg ccacgcttca gcgcccgcga
     4441 ggccttgtcg agtcggccgg cgaccccggc gcgggtgaac cgcacccgcc cttcgtcgac
     4501 gaaacgggcc gccatcgagc gcgtcttgaa gaagcgcgcg cgccacagcc agacatcggc
     4561 gcgacaggac tcgtcgctca tgatgcggcg ggcttgcgcc ggcggggccg gcgccgacgg
     4621 ggcggcgcga ccttcagccc ggccagggcg gcgaacggcg aatgggccgg cggc
//
LOCUS       DAORNX010000222         4653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13761_length_4653_cov_6.824924, whole genome shotgun sequence.
ACCESSION   DAORNX010000222 DAORNX010000000
VERSION     DAORNX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13761_length_4653_cov_6.824924"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLO65_10290"
     CDS             complement(<1..294)
                     /locus_tag="PLO65_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRD28676.1"
                     /translation="MAFDTPQARRTFAIVGGGFSGALLALKLTAARPDWRVALVDPSS
                     RPGRGLAYGACAPSHLLNVPVARMEAGLTPPFSDWLSGRPEMAEALAESDGAMS"
     gene            559..1176
                     /gene="rpsD"
                     /locus_tag="PLO65_10295"
     CDS             559..1176
                     /gene="rpsD"
                     /locus_tag="PLO65_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRD28677.1"
                     /translation="MSKRHSAKYKIDRRMGENIWGRPKSPVNSRSYGPGQHGQRRKSK
                     VSDFGLQLRAKQKLKGYYGNLTEKQFSRTYTEAARRKGNTAENLVGLLEARLDAVVYR
                     AKFVPTVFAARQFVNHGHVLVNGKRVNIASYRVKAGDVVSVREKSRNMALVLEALGSS
                     ERDTPDYITVDARHMAATFVRAPELAEVPYPVKMEPNLVVEFYAS"
     gene            1467..1841
                     /locus_tag="PLO65_10300"
     CDS             1467..1841
                     /locus_tag="PLO65_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28678.1"
                     /translation="MNRRVLLIAGALLLAGCATARVAPPEGAGFELLEPLYTVSTDDR
                     VLAIRVASNGCTKKEDFAFFVEKTPAGARVAFGRKTLDRCKSFAQGRVELMFTWEELG
                     LDGRAGVFVANPVVAWTGPGAL"
     gene            complement(1838..2245)
                     /locus_tag="PLO65_10305"
     CDS             complement(1838..2245)
                     /locus_tag="PLO65_10305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRD28679.1"
                     /translation="MLEPPAGRQVFTLPFHPKPSDIDENGHVNNVVYLGWVQDIAIAH
                     WRSRAPVEEAARWAWVVLRHEIDYRRPLMPGEDAVGRTWVGERRGPRFDRFVRIDGPD
                     GEMCAQTHSEWVLVDAVSKRPARVPAWMEEMFS"
     gene            complement(2248..2589)
                     /gene="grxD"
                     /locus_tag="PLO65_10310"
     CDS             complement(2248..2589)
                     /gene="grxD"
                     /locus_tag="PLO65_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRD28680.1"
                     /translation="MTDAAVANPAHDFIARTVTEHPVVLFMKGEPEQPRCGFSAQVVQ
                     ILDHLGVDFVGVDVLQSEVLRDGIKVYSDWPTIPQLYVKGEFVGGCDIVREMFQDGEL
                     KTLLESQGLLS"
     gene            complement(2649..3497)
                     /locus_tag="PLO65_10315"
     CDS             complement(2649..3497)
                     /locus_tag="PLO65_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28681.1"
                     /translation="MRYRPYGNSGHVVSAVSVLLDSAVRHSARDWRNLITAAMDCGIN
                     AFELAGDAPALIEGAAEALADIERNLLFIAWRPTSLPAEPAATVEALLTRTGLGYLDL
                     MNFDDVPPPSEVLARLKQGRWVRSFGLTSDDEDTDLLISSGLFDALTTAYSPVSGWRD
                     RNRLKAAAERDMAVIARDIWPEAMQPRSRLFKIPHLGRRANPLAGIGGYNFLETTPGW
                     SAEEICLAYVLTEPAVTTVRFQIDRIDRLVRLAEVSDRDLPTGVAAQIEMARFSSDGS
                     RIGARG"
     gene            complement(3501..3737)
                     /locus_tag="PLO65_10320"
     CDS             complement(3501..3737)
                     /locus_tag="PLO65_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HRD28682.1"
                     /translation="MPMPAADLESRLRTAFPGAEIVITDLAGDGDHYKARIVSPAFRG
                     LSRVRQHQLVNKALADVLGGALHALALETAAPAD"
     gene            3883..4524
                     /locus_tag="PLO65_10325"
     CDS             3883..4524
                     /locus_tag="PLO65_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28683.1"
                     /translation="MRVLLGLLLLLLLGGCNRVHSDHPLFFADDTADATHLRDGLWVL
                     EDSDSADCRFDARKPVTRWPDCADWMLVRGGEVLGYDPPGKDDATGAGAWTSLSVVLS
                     AGDPPILQVGMTEDGKVDYQFFGLEPTERAAGSITAFASWPVLCGPPPPADAMTGGKR
                     RYVSLEPLPGLTVIDESSCTADAAAAVRNAAGPSRAWAGDDAGGARWIRDTYP"
BASE COUNT          755 a         1585 c         1606 g          707 t
ORIGIN      
        1 gctcatcgcc ccgtcgctct cggccagggc ctcggccatt tcgggccgcc cgctcagcca
       61 gtcgctgaag ggcggggtca ggccggcctc catcctggcg accggcacat tgagcaggtg
      121 ggaaggggcg caggcgccgt aggccaggcc gcgtcccggc cggctcgacg gatcgaccag
      181 cgccacgcgc cagtccggcc gcgcggcggt caacttcagc gccagcagcg cgccactgaa
      241 ccccccgccg acgatggcga aggtcctgcg ggcttgaggt gtgtcgaatg ccatcggccc
      301 cgaggattcg aagattgccc gggggcatct aagggcagtt ggcgagtcgg ggggagggga
      361 gagttttggc tcccccgttg acgggaccga ggtgtagacg ttgactcccc gccacccctc
      421 catcataagc gcgccttcca acgccgccgg cttgccggac ggtgctggat ttcatgacgg
      481 gtgacacgaa gccgccgttg aaatcgcgcc tccactcacg ggggcgccgg cccgggtcga
      541 actgaagaag agctatttat gtccaagcgc cacagcgcca agtacaagat cgaccgccgg
      601 atgggcgaga acatctgggg tcgtccgaag tccccggtga acagccgctc ctacggcccc
      661 ggccagcatg gccagcgccg caagtccaag gtctccgatt tcggcctgca gctgcgcgcc
      721 aagcagaagc tgaagggcta ctacggcaac ctgaccgaaa agcagttcag ccgcacctac
      781 accgaagccg cgcgccgcaa gggcaacacg gccgagaacc tggtcggcct gctggaagcc
      841 cgcctcgacg cggtggtcta ccgcgccaag ttcgtgccga ccgtcttcgc cgcccgtcag
      901 ttcgtcaacc acggccacgt gctggtcaac ggcaagcgcg tcaacatcgc ctcctaccgc
      961 gtgaaggccg gcgacgtcgt cagcgtccgc gagaagtcgc gcaacatggc cctggtgctc
     1021 gaagccctgg gctcgtccga gcgtgacacc cccgactaca tcaccgtgga cgcccgccac
     1081 atggcggcga ccttcgtccg cgccccggaa ctggccgaag tgccctatcc ggtgaagatg
     1141 gagccgaacc tggtcgtcga attctacgcc tcgtaggatt gtcgccagac aaagttgcga
     1201 agggccccgg agcaatccgg ggcccttttg ctatggcgcg gccggcatct aaatctggtg
     1261 agggagagcc ctgaaggcct tgatggaatg gtccgctccg gacgtgacct tggtcaagga
     1321 ccgcttcgcg gccgcgcagc ggcgggccct gcgggcccgt ccttgacgaa ggtcccgccg
     1381 gagcaagaat gctcatcaag acatcaggtc tcatccgcct gttgcgaagt ctggacaagc
     1441 gcggcccgtc cgtctctcct ccccccatga accgccgcgt ccttctcatc gccggagccc
     1501 tgctgctcgc cggctgcgcc acggcccgcg tcgcgccgcc ggagggggcg gggttcgagc
     1561 tgctggagcc gctctacacc gtgagcacgg acgaccgggt gctggccatc cgtgtggcct
     1621 ccaacggctg tacgaagaag gaggatttcg ccttcttcgt cgagaagacg ccggccgggg
     1681 cgcgggtggc cttcggccgc aagacgctcg accggtgcaa gagcttcgcc cagggccggg
     1741 tcgaactgat gttcacctgg gaagagctgg gcctggacgg ccgtgccggc gtgttcgtgg
     1801 ccaatccggt ggtcgcctgg accggcccgg gcgcgctcta gctgaacatc tcttccatcc
     1861 acgccggcac ccgcgccggg cgcttgctga cggcgtcgac cagcacccac tccgaatgcg
     1921 tctgggcgca catctcgcca tcggggccgt cgatgcgcac gaaacggtcg aaacgggggc
     1981 cgcggcgctc gccgacccag gtgcggccga cagcgtcctc gccgggcatc agcgggcggc
     2041 gatagtcgat ctcgtggcgc agcacgaccc aagcccagcg cgcggcctcc tcgaccggag
     2101 cccgcgaacg ccagtgggcg atggcgatgt cctgcaccca gccgagatag accacgttgt
     2161 tcacatggcc gttctcgtcg atgtccgacg gcttgggatg aaacggcagg gtgaagacct
     2221 gccggcccgc gggcggctcg agcagtttca ggagagcaga ccctgggatt ccagcagcgt
     2281 cttcagctca ccgtcctgga acatctcgcg cacgatatcg cagccgccga cgaattcgcc
     2341 cttcacatac agctgcggaa tggtcggcca gtcgctgtag accttgatcc cgtcgcgcag
     2401 gacctcgctc tgcagcacat cgactccgac gaagtcgacg cccaggtggt cgaggatctg
     2461 cacgacctgg gccgagaagc cgcagcgcgg ctgttccggc tcgcccttca tgaacagcac
     2521 gaccgggtgt tcggtcacgg tgcgggcgat gaaatcgtgg gcgggattgg cgacagcggc
     2581 gtcggtcacg gcaggggcct ttgggcttgg aaattcacct tatcagctag gcgcccggcc
     2641 cggtccggtc aaccccgcgc gccgatccgc gagccgtccg acgagaaccg cgccatctcg
     2701 atctgggccg cgacgcccgt gggcaggtcg cggtcggaga cctccgccag ccggaccagc
     2761 cggtcgatac ggtcaatctg gaaccgcacg gtggtcacgg ccggctcggt cagcacatag
     2821 gccaggcaga tttcctcggc cgaccatccg ggggtcgtct cgaggaagtt gtagccgccg
     2881 atgccggcca gcgggttggc gcggcgcccg aggtgcggaa tcttgaacag ccgcgacctg
     2941 ggctgcatcg cctcgggcca gatgtcgcgg gcgatcacgg ccatgtcgcg ctcggccgcc
     3001 gccttcagcc ggttgcggtc gcgccagccc gacacggggc tgtaggccgt cgtcagggcg
     3061 tcgaacaggc cgctggagat cagcaggtcg gtgtcctcgt cgtcgctggt caggccgaac
     3121 gagcgcaccc agcgtccctg cttcagtcgc gccagcacct ccgacggggg cgggacgtcg
     3181 tcgaaattca tcaggtcgag atagccaagg ccggtccggg tcagcagggc ctccacggtc
     3241 gcggcgggtt cggcgggcag gctggtcggc cgccaggcga tgaacagcag gttgcgctcg
     3301 atgtcggcca gcgcctccgc cgcgccctcg atgagagcgg gcgcgtcgcc ggcgagttcg
     3361 aaggcgttga tgccgcagtc catggccgcg gtgatcaggt tgcgccagtc gcgggccgaa
     3421 tggcgaacag cgctgtcgag cagcacggac accgccgaca ccacatgacc ggaattgccg
     3481 tagggacggt agcgcatggg tcagtcggcc ggagcggcgg tttcgagcgc cagggcgtgc
     3541 agggcgccgc ccagcacatc ggccagcgcc ttgttgacca gctggtgctg gcgaacccgg
     3601 gacaggccgc ggaaggcggg cgagacgatg cgcgccttgt agtggtcgcc gtcgccggcc
     3661 aggtctgtga tcacgatctc ggccccggga aacgcggtgc gcaggcgaga ctcgagatcg
     3721 gcggcgggca tcggcacggg caggtctccg gaaatcagcc catttcaatg ggccagaatg
     3781 cttaacaacg gctggacggg cgccggcgct gcatcggaat ttgacgtgcg gccggtccgg
     3841 atcggcgcca tgctcacgcg tccgtgatcg gagggcgtcg ccatgagagt cctgctgggt
     3901 ctgcttctcc tgctgttgct gggtggctgc aaccgtgtgc acagcgacca tccgctgttc
     3961 ttcgccgacg acacggccga tgccacccac ctgagggacg ggctgtgggt gctcgaggac
     4021 agcgacagcg ccgactgccg cttcgacgcg cgcaagccgg taacccgctg gccggactgc
     4081 gccgactgga tgctggtgcg cggcggcgag gtgctgggct acgacccgcc cggcaaggac
     4141 gatgcgacgg gggccggcgc atggaccagc ctgtcggtcg tgctgtcggc cggcgatccg
     4201 ccgatcctgc aggtcggcat gaccgaggac ggcaaggtcg actaccagtt cttcggcctt
     4261 gagccgacgg aaagggcggc cgggtccatc acggccttcg ccagctggcc ggtcctgtgc
     4321 ggtccaccgc cgccggccga cgccatgacg ggcggcaagc gtcgctatgt gtcgctggag
     4381 cccttgccgg gcctgacggt gatcgacgag agcagctgca cggccgacgc cgccgccgcc
     4441 gtccgcaacg ccgccggccc cagccgcgcc tgggccggcg atgacgccgg cggcgcccgc
     4501 tggatccgcg acacctaccc ttaagcgtca gtcctcgatc gccgaataga ccaggctctt
     4561 gagctggccg cggaagttga acgtcgccga gggcatcaac tgggtcatga agaccaccga
     4621 gatgtcctcc ttcgggtcgg cccagaagat cgt
//
LOCUS       DAORNX010000223         4646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13783_length_4646_cov_6.532346, whole genome shotgun sequence.
ACCESSION   DAORNX010000223 DAORNX010000000
VERSION     DAORNX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_4646_cov_6.532346"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1223
                     /locus_tag="PLO65_10330"
     CDS             243..1223
                     /locus_tag="PLO65_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD28684.1"
                     /translation="MPFQSFTSVAEDAPALLWRGDANGRCVYLNRALRAFWGVPDDGV
                     ETFTWSSTLLAEDSDKVFGPFSDGMARRAPFRCEGRYRRADGEIRILETVAQPRFEDG
                     VFVGMVGVNTDVTEQRAAQAMLEASEARQRLLVHEMSHRVKNILATVLSIATMTSRSA
                     GSIGAFREAFEARLLALSKTHDLLTGKGGAHADLREVLEAELRPYSDLGGRRLSLEGP
                     SVPLDASSAVNLALIIHELATNAAKYGAYAADGALDVVWRRDPDGRVALDWRESGGPP
                     AEPPTTTGFGSRLIHGLVKGELAGDFAADYRTTGLVARLRFLPSQSPGSL"
     gene            1227..1883
                     /locus_tag="PLO65_10335"
     CDS             1227..1883
                     /locus_tag="PLO65_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRD28685.1"
                     /translation="MDTSATHYPTSAAKCADLVVHIVGLAFALFGGGILLGLAVARGA
                     PGQIGSVAVYAVGMALMLGCSLAYNFGKAPWRPLLRRFDHAGIFLMIAGSYTPFTTQS
                     LTGAWAIGMTAGVWTLALLGVAGKLFLPGLGKKFWVALYLALGWAVLVALKPMIDSVS
                     WVALLLLGIGGLLYSTGVIFYLMKTFHFRRAVWHGHVVAAAAVQYAAVLVGVVLAGAH
                     "
     gene            complement(1918..3753)
                     /locus_tag="PLO65_10340"
     CDS             complement(1918..3753)
                     /locus_tag="PLO65_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD28686.1"
                     /translation="MTRVSWTARGLSLITALWVTAAILASPARALADDTLTCAPAPFG
                     EKRVALVIGNAAYAAGNALTTPGSDATAVAAALCAHGFDVGRAWIDQRGPDLRTLLAD
                     ITERAQGADQVVVYYSGHGIEWKDGYSRFLGVDWDGSGEPPDIVAISITQIKQALADS
                     GARAKIIIIDACRNGEPPLETLKRAYSGGDVTPPAAGLQTITLYSTGPGKTSLSEAEG
                     GHSLFTGPLLRRLAEPGWPWLRLFNQVMLDVGATGDVRLPQSIGLIDDRLAPGVLTEQ
                     VRLNASDTARPAERPTHFAQLVTDARGHFVYWRLDPSASTPTPTDPGERYFLAALQAA
                     SRGGADAMVEAVGLLRRAADAGNVEARTNLAYHHATGRGVAVDITTAERLWREAAGLG
                     SAVAMDSLGVLERSVGDDGSSPFCDRSTGPTDWTEKDIAAVGWFQKAHDLGLAAATTD
                     LAFMKIMGRGTPRDCPGGRALLQAAAAADDPSGVGYLATALERGWGGPKDAAAARANF
                     VRARDLGDPDAMAWWAMDVLKTATGAQRRDAIALLERAAATGAPGAQYSAATWIEQFE
                     PCRLAEAATLYRLAAQGGYPSAYGRLAQIERLQAERGGSLACPPL"
     gene            complement(3767..4351)
                     /locus_tag="PLO65_10345"
     CDS             complement(3767..4351)
                     /locus_tag="PLO65_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28687.1"
                     /translation="MRIVRKIAFILALTAAQMTAVAPAMAAPVTIRFSTTEANSGESL
                     AAKLDSAMAQKAEKIVVVISPFTQTLPDPVAVWLKKIEQSGGEIYVRSLTMGLLIDII
                     GIVLPHVVVILREIVDNWLDSLGYGDPAKNYNAMIVTDKTGDGAATCFVRVEFYLRNS
                     AAWAAERAKGKPLSEASVSTEGTPAGTTQACPAG"
     gene            complement(4473..4548)
                     /locus_tag="PLO65_10350"
     tRNA            complement(4473..4548)
                     /locus_tag="PLO65_10350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4513..4515),aa:Thr,seq:cgt)
BASE COUNT          667 a         1657 c         1601 g          721 t
ORIGIN      
        1 cgtgacagcg acggcgtctg ggccccggcc tggtacgacg cggtcgaggc ctcgaccggc
       61 tttggccccc cgcgcgccga ggccggcccg gacgacctcg acggcgacct gcgccgcatc
      121 gccgacgcgg cgcgaccgca ctacgaacga ctggccgcct tccgcctctg accgtcggcc
      181 tccggcttga cggccgctcc ccattctgtg tttctgcgca ccgataaagg gcggggccgg
      241 acgtgccatt ccagagcttc acctccgtcg ccgaggacgc gcccgcgctg ctgtggcgcg
      301 gcgacgcgaa cggccgctgc gtctatctga accgcgccct gcgcgccttc tggggcgtcc
      361 cggacgacgg cgtggaaacc ttcacctggt cgtcgaccct gctcgccgag gacagcgaca
      421 aggtgttcgg cccgttcagc gacggcatgg cccgccgggc gccgttccgc tgcgagggcc
      481 gctaccgccg cgccgatggc gagatccgca ttctcgagac cgtcgcccag ccgcgcttcg
      541 aggatggcgt gttcgtcggc atggtcgggg tcaacaccga cgtgaccgag caacgcgccg
      601 cccaggcgat gctcgaagcc agcgaagccc gccagcggct gctggtccac gagatgagcc
      661 accgggtgaa gaacatcctc gccacggtgc tgtcgatcgc caccatgacc agccgctcgg
      721 ccggttcgat cggagccttc cgcgaggcgt tcgaggcgcg cctgctggcc ctgtccaaga
      781 cccacgacct gctgaccggc aagggcggcg cccacgccga tctgcgcgag gtgctggagg
      841 ccgagctgcg gccgtacagc gacctcggcg ggcgacggct gtcgctggag gggccgtctg
      901 tgcccctcga cgcctcgtcg gcggtcaacc tggccctgat catccacgag ctggcgacca
      961 acgccgccaa gtacggcgcc tatgccgccg acggcgcgct cgacgtcgtc tggcgccgcg
     1021 accccgacgg ccgcgtcgcg ctcgactggc gcgaatcggg cggccccccg gccgaaccgc
     1081 cgacgacgac gggcttcggc agccgcctga tccacgggct ggtcaagggc gaactggccg
     1141 gcgacttcgc ggcggactac cgaacaaccg gacttgtggc ccggctgcgc tttctgccgt
     1201 cacaatcgcc cggtagtctc tagggcatgg acacctccgc gacccactat ccgacgtcgg
     1261 ccgccaagtg cgcggatctg gtcgtgcata tcgtcggcct tgccttcgcc ctgttcggcg
     1321 gcgggatcct gctcggcctg gccgtggccc gcggcgcgcc ggggcagatc ggctcggtgg
     1381 cggtctacgc cgtgggcatg gcgctgatgc tcggctgctc gctggcctat aatttcggca
     1441 aggccccctg gcgaccgctg ctgcgacggt tcgaccatgc cggcatcttc ctgatgatcg
     1501 ccggcagcta cacgcccttc acgacccaga gcctgaccgg cgcctgggcc atcggcatga
     1561 ccgccggggt ctggacgctg gccctgctgg gcgtggcggg aaagctgttc ctgcccggcc
     1621 tgggaaagaa gttctgggtc gccctgtacc tcgccctcgg ctgggccgtg ctggtggccc
     1681 tcaagccgat gatcgacagc gtgtcctggg tcgccctgct gctgctgggc atcggcgggc
     1741 tgctctattc gaccggggta atcttctacc tgatgaagac gttccatttc cgccgggccg
     1801 tctggcacgg ccatgtggtc gccgcggcag ccgtgcagta cgccgccgtc ctggtcggcg
     1861 tggtgctggc cggcgcgcac taggccggcg acttccgccg cgcggacgcc gggcagacta
     1921 aagcggcgga caggccagac ttccgccccg ctcggcctga agccgctcga tctgcgcaag
     1981 ccgcccgtag gctgacgggt agccaccttg ggcggccagg cgatacaggg tcgccgcctc
     2041 ggccagccga cagggttcga attgttcgat ccaggtcgcg gccgagtact gggcgccggg
     2101 cgccccggtc gccgccgccc gttccagaag agcgatcgcg tcccgtcgct gggcgcccgt
     2161 ggcggtcttc aggacgtcca tagcccacca ggccatggcg tccgggtcgc ccaggtctcg
     2221 cgccctgacg aaattcgctc gcgccgccgc ggcgtccttg ggcccgcccc agccccgttc
     2281 aagcgccgtc gcgagatagc cgaccccgct gggatcgtcg gccgcggccg cagcctgcaa
     2341 cagggcccgc cccccggggc agtcgcgcgg cgttcctcgc cccatgatct tcatgaaggc
     2401 caggtcggtc gtcgcggcgg cgaggcctag atcatgcgcc ttctggaacc agccaacggc
     2461 cgcgatatcc ttctccgtcc agtccgtcgg accggtcgag cgatcgcaga aggggctgga
     2521 gccgtcgtcg ccgacggacc gttccagcac gcccagactg tccatggcaa ccgccgatcc
     2581 gagcccggcg gcctcgcgcc acagtcgttc cgccgtcgtg atgtcgaccg ccaccccacg
     2641 acccgtggcg tggtggtagg ccaggttggt ccgggcctcg acattgccgg cgtcggccgc
     2701 ccggcggagc agtcccacgg cctcgaccat tgcgtcggcg cccccgcggg acgcggcttg
     2761 caacgccgcc agaaaatagc gctcgcccgg gtcggtcggc gttggcgtgc tggccgaggg
     2821 atccaggcgc cagtagacaa aatggccacg cgcatcggtg accagctggg caaagtgggt
     2881 cggtcgctcg gcgggccgcg ccgtgtcgga cgcattcaga cggacctgtt cggtcagcac
     2941 accgggcgcc agccgatcgt cgatcagacc gatcgattgc gggagtcgca cgtccccggt
     3001 cgcgccaaca tcgagcatca cctggttgaa cagccgcagc cagggccaac ccggctcggc
     3061 gagccgccgc agcagcgggc ctgtgaacag cgaatggccg ccctcggcct ctgacaggct
     3121 cgtcttgccc ggccccgttg aatacagggt gatggtctgg agcccggcgg cgggcggcgt
     3181 cacgtcgccc ccgctatagg cccgcttgag tgtctccagc ggcggttcgc cgttgcggca
     3241 ggcgtcgatg atgatgatct tggcccgcgc gccactgtcg gccagggcct gcttgatctg
     3301 ggtgatcgag atcgcgacga tgtcaggcgg ctcgccggac ccgtcccagt cgacgcccag
     3361 gaaccggctg tagccgtcct tccactcgat gccatggccg gaatagtaga ccacgacctg
     3421 atcggcgccc tgggcccgtt cggttatgtc ggccagcaac gtcctgaggt ccgggccgcg
     3481 ctggtcgatc caggcgcgtc cgacgtcgaa gccgtgggcg cagagggctg cggcgacggc
     3541 agtcgcgtcg gacccgggcg tggtcagggc gttaccggcg gcataggccg cattgccgat
     3601 gaccagcgcg acccgctttt cgccgaacgg cgccggggcg caggtcaggg tatcgtcggc
     3661 cagcgcccgc gccgggcttg cgaggatcgc cgccgtgacc cacagcgccg tgatcagcga
     3721 caggcctcgg gcggtccacg aaacacgcgt cacgccggtc gctccactag cctgccgggc
     3781 aggcctgggt ggtcccagcc ggcgtgccct cggtcgacac cgaggcctcc gagaggggct
     3841 tgcccttggc ccgttcggcg gcccaggcgg cgctgttcct cagatagaat tcaacgcgca
     3901 cgaagcaggt cgccgcgccg tcaccggttt tatcggtcac gatcatggcg ttgtagttct
     3961 tcgccgggtc gccgtagccg agcgaatcca gccagttgtc gacaatttcc cgcaggatga
     4021 ccacgacgtg gggcaggacg atgccgatga tgtcgatcag caggcccatg gtcaggctgc
     4081 ggacatagat ctcgccgccg gactgctcga tcttcttcag ccagacggcg acaggatcgg
     4141 gcagcgtctg ggtgaagggc gagatcacga cgacgatctt ctcggccttc tgggccatgg
     4201 cgctgtcgag cttggccgcg agactctcgc ccgagttggc ctccgtggtg gagaaccgga
     4261 ttgtgacagg cgccgccatc gccggcgcga ccgccgtcat ctgggccgca gtcaacgcga
     4321 gaatgaaggc gatcttccgg acgatccgca tggcttcccc cccccaggag ctgaaagaac
     4381 gtcggtattt ggcggcgccg caaccgccgc gtcaatgccg accgccgccc cggggcggcg
     4441 gaaactgacg aaacacggat cggcgaagga aatggtgccg cttaggtgac tcgaacacct
     4501 gaccccatca ttacgaatga tgtgctctac cagctgagct aaagcggccc cggcggggga
     4561 tggccccgga aggagcgcct atttagcggc gcgagtcggg cttgccaagc gtcgccggcg
     4621 acgcggcggc gtggcgggcg gcggac
//
LOCUS       DAORNX010000224         4645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13789_length_4645_cov_6.746841, whole genome shotgun sequence.
ACCESSION   DAORNX010000224 DAORNX010000000
VERSION     DAORNX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_4645_cov_6.746841"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PLO65_10355"
     CDS             complement(<1..115)
                     /locus_tag="PLO65_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28688.1"
                     /translation="MSDIVIVSAARTPVGSFNGALSSLPAHELGKIAIQAAV"
     gene            337..990
                     /gene="phaR"
                     /locus_tag="PLO65_10360"
     CDS             337..990
                     /gene="phaR"
                     /locus_tag="PLO65_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRD28689.1"
                     /translation="MSEKPQTGENAAGDKSGGDRVVIKKYANRRLYNTASSSYVTLEH
                     LSDMVKEGVDFVVYDAKTGEDITRPVLTQIIFEEENRGGGQNLLPIQFLRQLIGFYGN
                     QMQAFVPSFLEMSLANFAKQQEQFRSQFGAMTPGAGLSGAGLGAYEEQIRQNMALFDR
                     AMKMFSPFGGYGSRAEESEAAARPAEAPAAGGDEALSDLKRQMAAMQAQLEKLASKG"
     gene            1026..1358
                     /locus_tag="PLO65_10365"
     CDS             1026..1358
                     /locus_tag="PLO65_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28690.1"
                     /translation="MTGSVDPVRPFERRQTRRRAEDLSRPAGADGGTNLPAVIEPAHA
                     EDLADPPPAEGYSTFAAQMYGQTGQKRGLRGGQPVIDQARAAYLETEYSGPDDRRPPK
                     GLLKKTEI"
     gene            complement(1407..1715)
                     /locus_tag="PLO65_10370"
     CDS             complement(1407..1715)
                     /locus_tag="PLO65_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28691.1"
                     /translation="MALFFDQSWFDARLADRGLSRDALAAVTGMSAADLALVWKDQRE
                     LSAAEVAVLAELLGVTGAEIAHRAGISTPVPGADPLARIAALERRVTALEAEVARLKG
                     "
     gene            complement(1850..2284)
                     /locus_tag="PLO65_10375"
     CDS             complement(1850..2284)
                     /locus_tag="PLO65_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28692.1"
                     /translation="MFAAFPLLALPVLVYNLVALTLRGGFGSQGASGKFTEPLFTVAM
                     TSGAPWPVSLSDLLLAGALVVLFIELLKSTTSRRIAIINHSLSMILFIVCLVEFLLAP
                     AFATSTFFLLTVMVLLDVLAGFIVTIVAARRDIDLAGGMDGN"
     gene            complement(2363..2533)
                     /locus_tag="PLO65_10380"
     CDS             complement(2363..2533)
                     /locus_tag="PLO65_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28693.1"
                     /translation="MEPFDLSDAGVVIGLVGLSIGVYCLYLKYRIDQLDRIIAEKEAA
                     LNAPEQPAATPR"
     gene            complement(2520..2756)
                     /locus_tag="PLO65_10385"
     CDS             complement(2520..2756)
                     /locus_tag="PLO65_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28694.1"
                     /translation="MVFAEFDRRARWTFAQRMKLGMQMLTTFAYASLGISVADSVLKG
                     SALNIGNLAAFAFGLVCAFLALHLVPQGERDGAF"
     gene            complement(2800..3615)
                     /locus_tag="PLO65_10390"
     CDS             complement(2800..3615)
                     /locus_tag="PLO65_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD28695.1"
                     /translation="MTEKTPAPRRKAASAAKAAAKTVATSAPKAAPATPDFSVVVPVF
                     DEGGAAPALAREIAGAFKGANFEMVFVNDASRDNTLELLKALKAELPMLRVLSHRRNA
                     GQSRSIRTGILAARAPIVVTLDGDGQNDPADAPRLAEALKAGPPELAMIGGERVKRQD
                     SAAKKIASRFGNGVRNWLLKDGATDTGCGLKAFRREAFLRLPYFDHIHRYLPALMIRE
                     GYQIAFQPVNHRHRETGVSKYTNFGRLKASASDLLGVMWLQSRARNPGGADEV"
     gene            3671..>4645
                     /locus_tag="PLO65_10395"
     CDS             3671..>4645
                     /locus_tag="PLO65_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRD28696.1"
                     /translation="MTFESRLDHWSRGWRGPLLAALIALVAGLPGVFAVPPLDRDEAR
                     FAQATAQMLETDDYVSINYQDQPRDKKPVGIHWLQALSVAVLSDASDRDIWPYRIPSL
                     LGAMLAAAACAWGAAAFFGSRAGLLAGAMMGASFLLSTEAGIAKTDAVLAGTTTLALA
                     AMGRLWFAHRGGPPAGWKTRLLFWLGIGLAALVKGPVGPLVALMAALALWASERKIDW
                     VRSLGWSWGPLLVLAIVGPWAWAITVASDGAFWGAAIGGDLAPKLVGGHERHGGLPGY
                     YLLLSPMLTFPGSLLLPAGLALGWTARREPGVRFALCWLIPTWLMFELL"
BASE COUNT          743 a         1546 c         1635 g          721 t
ORIGIN      
        1 cgacggcggc ctggatggcg atcttgccca gttcgtgggc cggcaggctc gacagggccc
       61 cgttgaacga cccgacaggc gtgcgggcgg ccgaaacaat aacgatgtcg ctcatggaat
      121 tctctccgtc gttccctgcg cggcgctccg gcggctacga gcccggagcg ctcaattttt
      181 gttgcgaggc cgcaccttgc atagaccggc ccggttcgtc acgccctcat tttgcagcgc
      241 aatatgctgc gcagcaaaac ggccctttcg tcatgccttt gcatccgcga tagtgcggcg
      301 ccaaaaagcg cgtgaagcgt tcttcaggga atggaaatgt ccgagaagcc ccagaccggc
      361 gaaaatgccg ccggagacaa aagcggcggc gatcgcgtgg tgatcaagaa atacgcgaac
      421 cgtcgcctct acaacaccgc gtcgagttcg tacgtcacgc tcgagcacct ctcggacatg
      481 gtcaaggaag gcgtcgattt cgtcgtctat gacgccaaga ccggcgagga catcacccgt
      541 ccggtgctga cgcagatcat cttcgaggaa gagaatcgcg gcggcggcca gaacctgctg
      601 ccgatccagt tcctgcgcca gctgatcggc ttctacggca accagatgca ggccttcgtg
      661 cccagcttcc tggagatgag cctggccaac ttcgccaagc agcaggaaca gttccgcagc
      721 cagttcgggg cgatgacgcc gggcgccggg ctcagcggcg cgggcctggg cgcctacgag
      781 gagcagatcc gccagaacat ggcgctgttc gaccgggcga tgaagatgtt ctcgccgttc
      841 ggcggctatg gaagccgcgc cgaggagagt gaagccgcgg cccggcccgc cgaggcgccg
      901 gcggctggcg gtgacgaggc cctgagcgac ctgaagcggc agatggccgc catgcaggcc
      961 cagctggaaa agctggcctc caagggctga ccacttcttt accaacggtg gcctaggctg
     1021 gcgacatgac cggctccgtt gatcccgtcc gcccgttcga acgccgccag acccgtcggc
     1081 gcgccgaaga tctgtcccgt ccggccgggg ccgacggcgg gacgaatctc ccggccgtga
     1141 tcgagccggc ccacgccgag gacctggccg atccgccgcc ggccgagggg tattcgacct
     1201 tcgccgccca gatgtacggc cagaccggcc agaagcgcgg cctgcgcggc ggccagccgg
     1261 tgatcgacca ggcgcgggcc gcctatctcg aaaccgaata ctccggcccg gacgaccgcc
     1321 gtccgcccaa gggactgttg aagaagaccg agatctagac ggccggcgcc attgctctcc
     1381 tgcgcgggag agggatgagg aagagcctag cccttcagcc tggcgacctc ggcttccagg
     1441 gccgtcaccc gccgctccag cgccgcgatg cgcgccagcg ggtcggcgcc cgggaccggc
     1501 gtcgagatcc cggcgcggtg ggcgatttcc gctccggtga cgccgagcag ctcggcaagg
     1561 acggccacct cggcggccga cagctcgcgc tggtccttcc agaccagggc cagatcggcc
     1621 gcgctcatgc ccgtcacggc cgccagggcg tcgcgggaca ggccacggtc agcgaggcgg
     1681 gcgtcaaacc aggactggtc gaagaacagg gccatgcgcg gccagagtgg cgccggtctt
     1741 gcttaaccag cggtgaatcg accgctggag ccgcccgtgt ctctcttcgc catccgtctg
     1801 ctggacgtcg ccgtcgtgac cttcatcttc actgtcctga cctgaccggt cagttgccgt
     1861 ccatgccgcc ggcgagatcg atgtcgcggc gggcggcgac gatggtgacg atgaagccgg
     1921 ccagcacgtc gagcaggacc atcaccgtca gcaggaagaa ggtcgaggtg gcgaaggccg
     1981 gggccagcag gaactcgacc aggcagacga tgaacaggat catcgacagc gagtggttga
     2041 tgatcgcgat ccgccggctg gtggtcgact tcagcagttc gatgaacagc accaccaggg
     2101 cgccggccag cagcaggtcc gacagactga ccggccaggg cgcgcccgag gtcatggcca
     2161 cggtgaacag gggctcggtg aacttgccgc tggcgccctg ggatccgaag ccgccccgca
     2221 gcgtcagggc gacgagattg tagaccagga ccggcagggc cagcagcgga aacgcggcga
     2281 acatgggcag atctccccaa ccccggataa cccggttcgc aacggttaac gtctttccgc
     2341 ccgggggtga agcgggctcg cctcagcggg gcgtggcggc cggctgctcg ggagcgttca
     2401 gcgccgcttc cttctcggcg atgatgcggt cgagctggtc aatcctgtat ttcaggtaca
     2461 ggcagtagac gccgatcgag aggcccacga gcccgatcac aacgccggcg tccgagagat
     2521 caaaaggctc catcgcgttc tccctgaggc acaagatgga gcgcgaggaa ggcgcaaacc
     2581 agaccgaacg cgaatgcggc cagattgccg atgttgagcg cagagccttt cagcacggaa
     2641 tcggcgaccg atatgcccag agaggcgtag gcgaacgtcg tgagcatctg catgcccagc
     2701 ttcattcgct gcgcgaaagt ccatcgcgct cgccggtcaa attccgcgaa caccatgctc
     2761 catctcccga cggaacataa ccggaacaac cgtcgattgt caaacctcgt cggcgccgcc
     2821 ggggttgcgg gcgcgggact gcagccacat cacgccgagc aggtcgctcg ccgaggcctt
     2881 gagccggccg aaattggtgt atttcgacac gccggtctcg cggtggcggt ggttcaccgg
     2941 ctggaaggcg atctggtagc cctcgcggat catcaacgcc ggcaggtagc ggtggatgtg
     3001 atcgaaatag ggcaggcgca ggaaggcctc gcggcggaag gccttcaggc cgcagccggt
     3061 gtcggtcgcc ccgtccttca gcagccagtt gcgcacgccg ttgccgaagc gcgaggcgat
     3121 cttcttggcc gcgctgtcct ggcgcttgac ccgttcgccg ccgatcatcg ccagctccgg
     3181 cgggccggcc ttcagcgcct cggccagccg tggggcgtcg gccgggtcgt tctggccgtc
     3241 gccgtcgagg gtgacgacga tcggggcccg cgcggccagg atgccggtgc ggatcgagcg
     3301 gctctggccg gcgttcctgc ggtgcgacag gacccgcagc atcggcagtt cggccttcag
     3361 cgccttgagc agctccaggg tgttgtcgcg gctggcgtcg ttgacgaaca ccatctcgaa
     3421 gttcgcgccc ttgaaggcgc cggcgatctc gcgggcgagg gccggggcgg cgccgccctc
     3481 gtcgaacacc ggcacgacga cggagaagtc gggcgtcgcc ggcgcggcct tcggggccga
     3541 cgttgcgacg gtcttcgcgg cggctttcgc ggcggaggcg gccttgcggc gcggcgcggg
     3601 agtcttttcg gtcatggccc cctatagccg aaaagacggc gcaggggagg atcgtgctat
     3661 cagacggtcc atgacgttcg aatcccgcct cgaccactgg agccgcggct ggcgcgggcc
     3721 gctgctggcc gcgctgatcg cccttgtcgc cggactgccc ggcgtgttcg ccgtgccgcc
     3781 gctggaccgg gacgaagccc gtttcgccca ggccacggcg cagatgctcg agaccgacga
     3841 ctacgtctcg atcaactacc aggaccagcc gcgcgacaag aagcctgtcg gcatccactg
     3901 gctgcaggcg ctcagcgtgg cggtcctgtc cgacgcgtcg gacagggata tctggcccta
     3961 ccgcataccc tccctgctcg gggcgatgct tgcggcggcg gcctgcgcct ggggcgcggc
     4021 ggccttcttc ggctccaggg ccggcctgct ggccggggcg atgatggggg ccagtttcct
     4081 gctgtcgacc gaggccggca tcgccaagac cgatgcggtg ctggccggga ccaccaccct
     4141 ggcgctggcg gccatgggac ggctgtggtt cgcgcatcgc ggcggacctc cggccggctg
     4201 gaagacgcga ttgctgttct ggctgggcat cggcctggcg gccctggtca aggggccggt
     4261 cgggccgctg gtggcgctga tggccgccct ggccctgtgg gcctcggagc ggaagatcga
     4321 ctgggtccgg tcgctcggct ggagctgggg gccgctgctg gtgctggcca tcgtcgggcc
     4381 gtgggcctgg gcgatcaccg tggccagcga cggggccttc tggggcgcgg cgatcggcgg
     4441 cgacctggcg cccaagctgg tcggcggcca tgagcggcat gggggcctgc ccggctacta
     4501 tctgctgctg tcgccgatgc tgacctttcc cggcagcctg ctgctgcctg ccgggctggc
     4561 gctgggctgg accgcgcggc gcgagcccgg ggtgcgcttc gccctgtgct ggctgatccc
     4621 cacctggctg atgttcgagc tgctg
//
LOCUS       DAORNX010000225         4622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13869_length_4622_cov_5.270637, whole genome shotgun sequence.
ACCESSION   DAORNX010000225 DAORNX010000000
VERSION     DAORNX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13869_length_4622_cov_5.270637"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..222
                     /locus_tag="PLO65_10400"
                     /pseudo
     CDS             148..222
                     /locus_tag="PLO65_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            219..1136
                     /locus_tag="PLO65_10405"
     CDS             219..1136
                     /locus_tag="PLO65_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28697.1"
                     /translation="MTVIRDWTPDKAHAFGKEVLTFSHDLHSRPMFDDDGLADLLDRY
                     PRERLGVFTMGEDPRDWRTWRRGVAGALPGRTLLAMAREGRIWLNLRGASDHLPDYAA
                     LAEEVFAEKEARVPGLRTMKRDVGVLISSPNAQVFYHLDVPMVSLWQIRGVKRVWAYP
                     VEAPYVGAEALEAIVLRETAEQFAFDPAWDAGAAMTELTPGRMLTWPQNAPHRIENGP
                     MLNVSLSMEFMTPAALLRANVIYANGLLRRRLGADPAIQPGFSPAGLAKVAAARAAKA
                     LKLQKAHERVLPVTFNLETEVPGLAKRAA"
     gene            1200..1730
                     /locus_tag="PLO65_10410"
     CDS             1200..1730
                     /locus_tag="PLO65_10410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRD28698.1"
                     /translation="MTGMTTARTIDLTGEAAADARALRDAFGCFTTGVTIVTTLDGGA
                     PAGFTANSFASVSLDPPLALICVGAEASCLPALNTSGVFVVNVLHVDQQDLARQFARR
                     GCDRFEGVAFDRWHTGAPVLPGCMANFECRTHAVLDAGDHRIIIGRILKVHHDADHEP
                     LVFLRGGYRKVHTDQG"
     gene            1988..3160
                     /locus_tag="PLO65_10415"
     CDS             1988..3160
                     /locus_tag="PLO65_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD28699.1"
                     /translation="MGEAYIVAAGRTAGGRKGGRLSGVHPADLAGEVLKALVARTGAD
                     PALVEDVIMGCVGQAGEQAVNIARNAVLASGFPESVPATSVDRQCGSSQQSLHFAAQA
                     VMSGAMDCVIAAGVESMSRVPMGTPSILALKAGMGSYMSPDIQARYPNIQFSQFAGAE
                     MIAKKYGFTKDQLDEWAYGSHQKAIAATKAGHFDKEIVPVEITTAEGAKEQHVVDEGI
                     RFDASLEGIKGVKLLQEGGTVTAATSSQICDGASGLLVVNEAGLKRLGVEPLARVHHM
                     TVTAGDPVVMLEEPVFATQKALKKAGMKISDIDLYEVNEAFAPVPLAWLQGNDADPDR
                     LNISGGAIALGHPLGGSGAKLMATLIHNLRRTGKRYGLQTMCEGGGLANVTIVEAL"
     gene            complement(3385..>4374)
                     /locus_tag="PLO65_10420"
     CDS             complement(3385..>4374)
                     /locus_tag="PLO65_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HRD28700.1"
                     /translation="SAQGPLLADLTGDGRPELRAVFLKGLNSPFGVALVGDTLYVANT
                     DSIMAFPYATGATAITAAGAKIVDLPANDPNPHWARNILASPDGTKLFVTVGSDSNIA
                     DGGIEAEQDRANILELDLATKALRVFASGLRNPNGLAWEPVSGALWTTVNERDMLGDD
                     LVPDYMTSVKDGGHYGWPWSYYGQHVDDRVQPQRPDMVARAIVPDYALGAHTASLGLA
                     FWPAPPTGPGMPECDERGCVPASYGLNLRYAGGALVGQHGSWNRKPVSGYKVVYVPFK
                     DGKPAGAAQDFLTGFLDSKGQARGRPVGVAFDHTGAALVADDVGNVVWRVSAK"
     assembly_gap    4375..4384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4385..>4622)
                     /locus_tag="PLO65_10425"
     CDS             complement(<4385..>4622)
                     /locus_tag="PLO65_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HRD28701.1"
                     /translation="GWPAGGAPSAPAGFTVTRYAEGLAHPRWLYVLPNGDVLVVESNS
                     PPRKKTSLTDWVAAAVFKRAGADAPSANRITLLRD"
BASE COUNT          711 a         1640 c         1559 g          702 t
ORIGIN      
        1 cgaaggcgcc cacgaagtcc agcagggccg cgcctttgat ggcctgtccg atgcgctgca
       61 tcatggggtg gccgccttcc tgggctgaat ctcgcagata tagttggaca gctgggccat
      121 atcgccgccg gcgtccttgg gccgcaccgc gccgcccgcg gactcggcaa gaccttctgg
      181 ttcggtccct tcaagatcaa tttctatgga cgcagcgcat gaccgtcatt cgcgactgga
      241 ccccggacaa ggcccacgcc ttcggcaagg aggtgctgac cttcagccac gacctgcaca
      301 gccggccgat gttcgacgac gacggcctgg ccgacctgct cgaccgctat ccgcgtgagc
      361 ggctcggcgt gttcaccatg ggcgaggacc cgcgcgactg gcgcacctgg cgccgcggcg
      421 tggctggagc cctgccgggg cgcaccctgc tggccatggc ccgcgaaggc cgcatctggc
      481 tgaacctgcg cggcgccagc gaccaccttc cggactacgc cgccctggcc gaggaggtgt
      541 tcgccgagaa ggaggcccgg gtcccgggcc tgcgcacgat gaagcgcgac gtcggcgtgc
      601 tgatcagctc gccgaacgcc caggtcttct accatctgga cgtgccgatg gtgtcgctgt
      661 ggcagatccg cggcgtcaag cgggtctggg cctatccggt cgaggcgccc tacgtcgggg
      721 ccgaggcgct ggaggccatc gtgctgcgcg agacggccga gcagttcgcg ttcgatccgg
      781 cgtgggacgc gggcgcggcg atgaccgaac tgacccccgg ccgcatgctg acctggccgc
      841 agaacgcgcc ccaccggatc gagaacgggc cgatgctcaa cgtctccctg tcgatggagt
      901 tcatgacccc cgcggcgctg cttcgggcca atgtgatcta cgccaacggc ctgctgcgcc
      961 ggcggctggg cgccgacccg gccatccagc ccggcttttc gccggccggc ctggccaagg
     1021 tcgccgccgc ccgcgcggcc aaggcgctga agctgcaaaa ggcccacgaa cgcgtgctgc
     1081 cggtgacgtt caatctggag acggaagtgc cgggactggc gaagcgggcg gcttagtcac
     1141 gaccctcgcg aggcaaggtg gcggccttct cccttgcccc gcctcaccga tcgctccaaa
     1201 tgaccgggat gaccaccgcc cgcacgatcg acctgactgg cgaggccgcc gccgatgcgc
     1261 gggccctgcg cgacgccttc ggctgcttca ccaccggggt gaccatcgtc acgacgctcg
     1321 atggcggggc gccggccggc ttcaccgcca acagcttcgc ttcggtgtcg ctcgacccgc
     1381 cgctggcgct gatctgcgtc ggcgccgagg ccagttgcct gccggccctc aacaccagcg
     1441 gcgtcttcgt ggtcaatgtc ctgcatgtcg accagcagga tctggcccgc cagttcgcgc
     1501 gtcggggctg cgaccgtttc gagggcgtgg ccttcgaccg gtggcacacg ggcgcgcccg
     1561 tcctgcccgg ctgcatggcc aatttcgagt gtcgcaccca tgcggtcctc gacgccggcg
     1621 accaccgcat catcatcggt cgcatcctga aggtgcacca cgacgccgac cacgagccgc
     1681 tggttttcct gcgcggcggc taccggaagg tgcatacgga ccaggggtag ggcgtaggga
     1741 gtagggagta gggagtaggg agtagggagt agcaggaggg actagtggct agggactagt
     1801 aggtccacgc gttcgccgcc acctcgctca actactccct actccctact ccctaacccc
     1861 taacccctaa cccctaaccc ctggcccctg gtcccttccc cctgacgctg ggtcatactt
     1921 ggcgcgggac atccgaaggc ttaagtgacg ttcacgtaaa cgaaaccctc caaccgggag
     1981 ataccccatg ggtgaagcct atatcgtcgc cgccggccgc accgcgggcg gtcgcaaggg
     2041 tggccgtctg agcggggtcc accccgccga cctggccggc gaggtcctca aggccctggt
     2101 cgcgcgcacc ggcgccgatc cggccctggt cgaggacgtg atcatgggct gcgtgggcca
     2161 ggccggcgaa caggcggtca acatcgcccg caacgccgtg ctcgccagcg gcttcccgga
     2221 aagcgtgccg gctacctcgg tcgaccgtca gtgcggctcg tcgcagcagt cgctgcattt
     2281 cgccgcccag gccgtcatgt ccggcgccat ggactgcgtc atcgccgccg gcgtcgagag
     2341 catgagccgc gtgccgatgg gcaccccgtc gatcctggcc ctcaaggccg gcatgggcag
     2401 ctacatgagc ccggacatcc aggcccgcta tccgaacatc cagttcagcc agttcgccgg
     2461 cgccgagatg atcgccaaga agtacggctt caccaaggac cagctcgacg agtgggccta
     2521 cggcagccac cagaaggcca tcgccgccac caaggccggt cacttcgaca aggaaatcgt
     2581 gcccgtcgag atcaccaccg ccgagggcgc caaggagcag catgtcgtcg acgagggcat
     2641 ccgcttcgac gccagccttg aaggcatcaa gggcgtgaag ctgctgcagg aaggcggcac
     2701 ggtcacggcc gccacctcga gccagatctg cgacggcgcc tcgggcctgc tggtggtcaa
     2761 cgaggccggc ctcaagcgtc tcggcgtcga gccgctggcc cgcgtgcacc atatgaccgt
     2821 caccgccggc gaccccgtcg tgatgctgga agagccggtg ttcgccaccc agaaggcgct
     2881 caagaaggcc ggcatgaaga tcagcgacat cgacctgtac gaggtcaacg aggccttcgc
     2941 gccggtgccg ctggcctggc tgcagggcaa cgacgctgat ccggacaggc tgaacatctc
     3001 gggcggcgcc atcgcgctgg gccacccgct cggcggctcg ggcgccaagc tgatggccac
     3061 cctgatccac aacctgcgtc gcaccggcaa gcgctacggc ctgcagacca tgtgcgaagg
     3121 cggcggcctg gccaacgtga cgatcgtcga ggcgctctga tcgcgccctg accgtctgct
     3181 gaaaagggcc gcccaccgga aggtgggcgg tcccgttttt cttgttcctc tccgcgaagc
     3241 ggggaggggg accatgcgga gcatggtgga ggggccggcg ccgaggcgag cggtcccctt
     3301 ctgaattcag gtccttccgg cgccgtcggc gcagccccct ccaccagcct ccggctggtc
     3361 cccctcacct ctacgagggg aggatcattt cgcgctcacc cgccacacga cattgccgac
     3421 gtcatcggcc accagcgccg cgccggtgtg gtcgaaggcg acgccgaccg ggcggccgcg
     3481 cgcctggccc ttgctgtcga ggaagccggt caggaagtcc tgcgccgcgc cggccggctt
     3541 gccgtccttg aagggcacat agaccacctt gtagccgctg accggcttgc ggttccacga
     3601 gccgtgctgg ccgaccaggg cgccgccggc gtaacgcagg ttcaacccgt aggatgccgg
     3661 cacgcagccg cgctcgtcgc actcgggcat gcccggaccc gtcgggggcg ccggccagaa
     3721 ggccagcccc agcgacgccg tatgggctcc cagcgcgtag tcggggacga tggccctggc
     3781 caccatgtcc ggccgctgcg gctgcacccg gtcgtcgaca tgctggccgt agtagctcca
     3841 cggccagcca tagtggccgc cgtccttcac gctggtcatg tagtcgggga ccaggtcgtc
     3901 gccgagcatg tcgcgctcgt tgaccgtggt ccacagggcg ccgctgaccg gctcccaggc
     3961 caggccgttg ggattgcgca ggccgctggc gaacacccgc agggccttcg tcgccaggtc
     4021 cagctcaagg atgttggccc ggtcttgctc cgcctcgatg ccgccgtcgg cgatattgga
     4081 atccgagccg acggtcacaa acagtttcgt cccgtccggg ctggccagga tgttgcgcgc
     4141 ccagtgcgga ttgggatcgt tggccggcag gtcgacgatc ttcgcccccg ccgccgtgat
     4201 cgccgtcgcg cccgtcgcat agggaaaggc catgatgctg tcggtgttgg cgacatagag
     4261 ggtgtcgccg accagggcga cgccgaacgg cgagttgagg cccttcagga acaccgcgcg
     4321 cagttccggc cggccgtcgc cggtaaggtc cgctagcaac gggccctgtg cactnnnnnn
     4381 nnnncgtcgc gcagcagggt gatgcgattg gcgctcggcg cgtcggcccc ggcgcgcttg
     4441 aacaccgccg cggcgaccca gtcggtcagg ctggtcttct tgcgcggcgg cgagttgctc
     4501 tcgaccacca gcacgtcgcc attgggcagg acgtacagcc agcgcggatg ggccaggccc
     4561 tcggcgtacc gcgtcacggt gaagccggcg ggcgccgacg gggcgccgcc ggccggccag
     4621 cc
//
LOCUS       DAORNX010000226         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13884_length_4618_cov_7.906640, whole genome shotgun sequence.
ACCESSION   DAORNX010000226 DAORNX010000000
VERSION     DAORNX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13884_length_4618_cov_7.906640"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLO65_10430"
     CDS             <1..310
                     /locus_tag="PLO65_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRD28702.1"
                     /translation="EGEGGPAAVRAMGLHLARLRRILTLAQNGASLAEAAKASGVFWK
                     NEREMLRQARAWTLEHLLALHPEVLAADLACKTAGSPDHLLAERLALTIAGRARRLGL
                     "
     gene            complement(437..1318)
                     /locus_tag="PLO65_10435"
     CDS             complement(437..1318)
                     /locus_tag="PLO65_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRD28703.1"
                     /translation="MAEGRRGLGRGLSALLGESDAENPAATDAAAGAREIPIELIRRN
                     PDQPRRRFGEAEIAELADSIREKGVLQPVLVRPAPDAPGEYQLVAGERRWRAAQQAGL
                     KTMPALVRELDDQQVLEIAIIENVQRADLGPIEESLSYRALMDRFGRTQEEVARVVGK
                     SRPHVANALRLLSLPPEVQALLADGQLTAGHARALVGSLNPLAFAEEVIARGLNVRDT
                     EALVKRGAPEKKSAGPGGVGKVTRDHDTAALESDLSEVLGLEVEIRDRGGVGELRVRY
                     ATLEQLDDLCRRLTRGV"
     gene            complement(1336..2124)
                     /locus_tag="PLO65_10440"
     CDS             complement(1336..2124)
                     /locus_tag="PLO65_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD28704.1"
                     /translation="MRVLAIANQKGGVGKTTTAINLGTALAAVGERVLLIDADPQGNA
                     STGLGVPRQQRRVTLYDVMMGDTPITEAAIHTALPGLDLIASDADLSGVELELGQQAR
                     RSYRLRDAMERLRAAGSYSYVLIDCPPSLNLLTVNAMTASDAVFVPLQCEFFALEGLT
                     QLMRTVDLVRGSLNPALEIQGVVLTMYDRRNSLSEQVARDVRSHFGDKVYETVIPRNV
                     RVSEAPSFGKPVLIYDLKCAGSQAYLKLAREVVGRERRRRLAAA"
     gene            complement(2138..2791)
                     /gene="rsmG"
                     /locus_tag="PLO65_10445"
     CDS             complement(2138..2791)
                     /gene="rsmG"
                     /locus_tag="PLO65_10445"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRD28705.1"
                     /translation="MSDAPAPPEAVTAAQDAADFQALTGATDAQMADLERFRALLEAG
                     NAVMNLVGPATLPDFWNRHAWDSAQLLRLAPDARSWADLGAGAGFPGLILAILLKGTP
                     GARVQLVESMAKRCRFLDQVARDLALPVQIHNARAEDLSLTVDIVTARACAPMVRLLG
                     YARPYFQRGARGLFLKGQDVVTELNEAASSWTIKARLVPSLSDARGQIVDVESLARA"
     gene            complement(2766..>2956)
                     /locus_tag="PLO65_10450"
     CDS             complement(2766..>2956)
                     /locus_tag="PLO65_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRD28706.1"
                     /translation="RDEALRLPGDLDYGAVGGLSNEIRDKLTAIRPLTLGQAARIEGV
                     TPGALTALLAHVRRPRAA"
     assembly_gap    2957..2993
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2994..>4618)
                     /gene="mnmG"
                     /locus_tag="PLO65_10455"
     CDS             complement(<2994..>4618)
                     /gene="mnmG"
                     /locus_tag="PLO65_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRD28707.1"
                     /translation="AWDVIVIGGGHAGCEAAAASARFGARTLLLTHKLETIGEMSCNP
                     AIGGLGKGHLVREIDALDGLMGRMADKAGIQFRMLNRSKGPAVRGPRSQIDRRLYREA
                     MQAELAGQVNLEIIADAVEDLIVEDGVLTGVVGGSGAIYSAPRVVLTTGTFLKGVIHL
                     GDKRIPAGRVGDAPAIGLADRLYSLGLDMGRLKTGTPARLVGSTIAWDRLEMQAADET
                     PVPFSFLNRDITVPQIACGVTWTNAETHRIIADRLTESAVYGGRATGRGPRYCPSIED
                     KVVRFAEKTSHQIFLEPEGLDDDTVYPNGVSTSVSEETQLLFLRTIAGLEQVEVRRFG
                     YAIEYDYVDPRELFPTLEVKRLPGLYLAGQINGTTGYEEAGAQGLVAGLNAARAASGA
                     DPATFARDEAYIGVLIDDLVTRGVTEPYRMFTSRAEFRLTLRADNADQRLTGRGLALG
                     CVGPARAAVFHVKQTALAEARALARSLTLTPAAAEKAGLAVKADGQRRDVLQLLAYPD
                     IDLDRLALIWPELAAWTPEIREQIEIDAAYAGY"
BASE COUNT          681 a         1625 c         1583 g          692 t
ORIGIN      
        1 cgaaggggag ggcggaccgg ccgccgtccg ggccatgggc ctgcacctgg cccggctgcg
       61 ccgcatcctg accctggcgc agaacggcgc ctccctggcc gaggcggcca aggcctccgg
      121 agtgttctgg aagaacgagc gcgagatgct gcgtcaggcc cgcgcctgga cgctcgaaca
      181 tctgctggcc ctgcatcccg aagtgctggc cgccgacctt gcctgcaaga ccgccggatc
      241 gccggatcat ctgttggcgg agcgtctggc gctgacgatc gccggtcggg cccgtcgtct
      301 cggcctctga cgcctgcgcg cgcgagacgc cgaaaatttc ccgcattaat tcaacgactt
      361 aaaaagtcga atttgaaaat tgcctctgtg gcgaaataag ccgcgttcgg cgcaccgggt
      421 tacggctccg tgcggctcac actcccctcg tcagccggcg acagaggtcg tccagctgct
      481 cgagggtggc gtagcgaacc cgtagctcgc cgacgccgcc acggtcacgg atctcgacct
      541 cgaggccgag cacttccgag aggtcgctct cgagcgcggc ggtgtcgtgg tcgcgcgtga
      601 ccttgcccac gccgcccggc ccggcggact tcttctccgg cgcgccccgt ttcaccagcg
      661 cctcggtgtc acggacgttg agtccccggg cgatgacctc ctcggcgaag gccagcgggt
      721 tgagcgaccc gaccagcgcc cgcgcatggc cggcggtcag ctggccatcg gcgagcaggg
      781 cctgcacctc gggcggcagg ctcagcaacc gcaacgcgtt ggccacatgc ggccggctct
      841 tgccgaccac ccgcgcgacc tcctcctggg tgcgaccgaa acggtccatc agggcgcggt
      901 agctgagcga ctcctcgatc ggacccaggt cggcgcgctg gacgttttcg atgatggcga
      961 tctcgagcac ctgctggtcg tcgagctcgc gcaccagggc cggcatggtc ttgaggccgg
     1021 cctgctgcgc cgcccgccat cggcgctcgc cggcgaccag ctggtactcc ccgggcgcgt
     1081 ccggcgcggg ccggaccaga accggctgca acacgccctt ttcgcggatc gaatcggcca
     1141 gttcggcaat ctcggcctca ccgaaccgtc ggcgcggctg gtcggggttg cgccggatca
     1201 gctcgatcgg gatctcccgc gcgcccgcgg cggcgtcggt cgcggccgga ttctccgcgt
     1261 cggactcgcc cagcagggcg gacagacccc gacccagacc cctgcgacct tcggccatgg
     1321 acacgttggc tccgttcatg cggcggcgag gcggcgccgg cgttcgcgcc cgacgacttc
     1381 gcgggccagt ttcagatagg cttgcgatcc ggcgcacttc agatcgtaga tcagcaccgg
     1441 cttgccgaag gacggcgcct cggacacccg cacattgcgc ggtatgaccg tctcatagac
     1501 cttgtcgccg aagtggctcc gcacgtcgcg cgcgacctgt tccgacaggc tgttgcggcg
     1561 atcgtacatg gtcaggacca cgccctggat ctccagcgcc ggattcaggc tgccgcggac
     1621 cagatcgacc gtacgcatca gctgggtcag gccctccagc gcgaagaact cgcactgcag
     1681 cggcacgaac accgcgtccg aggccgtcat ggcgttgacg gtcagcaggt tcagcgaggg
     1741 cgggcagtcg atcagcacat agctgtagct ccccgccgcc cgcaggcgct ccatggcgtc
     1801 gcgcaggcgg taggaccggc gcgcctgctg gcccagctcg agctcgacac ccgacaggtc
     1861 ggcgtccgag gcgatcaggt ccaggccggg cagggcggtg tgaatcgccg cctcggtgat
     1921 cggggtatcg cccatcatca cgtcatagag cgtcacccgg cgctgctggc gcggcacgcc
     1981 caggccggtg gaggcgttgc cctgggggtc cgcgtcgatc agcagcaccc gctcgccgac
     2041 ggccgccagg gccgtgccga ggttgatcgc cgtggtggtc ttgcccacgc cccccttctg
     2101 gttggcaatg gccaggacgc gcatggactt ttggctgtca ggcacgggcc agggactcca
     2161 catccacaat ctgtccccgg gcatcgctca ggctggggac gagccgggcc ttgatcgtcc
     2221 aggacgacgc ggcctcgttc agttccgtta ccacatcctg tcccttgagg aacaatccgc
     2281 gggccccgcg ctggaagtag ggccgggcat acccgagcag tcgcaccatc ggcgcgcaag
     2341 cccgggcggt gacgatatcc acagtcagtg acaggtcttc cgcacgcgcg ttgtgaatct
     2401 ggaccggcag ggccaggtcg cgcgccacct ggtccaggaa gcggcagcgc ttggccatgc
     2461 tttccaccag ttggaccctg gcccccgggg tccccttgag caggatcgcc agaatcaggc
     2521 ccgggaagcc tgcgccggcc ccgagatcag cccaggaacg ggcgtcaggc gccagccgca
     2581 gcagctgggc gctgtcccag gcgtgacggt tccagaagtc gggcagggtc gcggggccga
     2641 cgaggttcat caccgcgttg ccggcctcca gcagcgcgcg aaaccgttcg aggtcggcca
     2701 tctgggcgtc ggtggcgccg gtcagggcct ggaaatcggc ggcgtcctgc gccgcggtga
     2761 ccgcctcagg cggcgcgggg gcgtcggaca tgggcgagca gggcggtcag ggcgccgggc
     2821 gtaacgccct cgatgcgggc cgcctggccg agggtcaggg ggcgaatggc ggtcagcttg
     2881 tcgcggatct cgttcgacag cccgccgacc gcgccatagt cgaggtcgcc gggcaaccgc
     2941 agcgcctcat cgcggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatagcc
     3001 ggcataggcc gcgtcgatct cgatctgctc gcggatttcc ggggtccagg cggccagctc
     3061 gggccagatc agcgccagcc ggtccaggtc gatgtcggga taggcgagca gctgcagcac
     3121 gtcgcgccgc tgaccgtcag ccttgacggc gaggccggcc ttctcggccg cggccggcgt
     3181 cagggtcagc gaccgggcca gggcgcgggc ctcggcgagc gccgtttgtt tcacgtgaaa
     3241 cacggcggcc cgcgccggcc ccacgcaacc cagcgccaag ccgcgcccgg tcagccgctg
     3301 gtcagcattg tcggcccgca aggtcaggcg gaactcggcc cggctggtga acatgcgata
     3361 gggctcggtg acccctcgcg tgaccaggtc gtcgatcagc acgccaatat aggcctcgtc
     3421 gcgcgcgaac gtcgccgggt ccgcgcccga agccgcacgg gccgcgttga gacccgccac
     3481 cagaccctgc gccccggcct cttcatagcc cgtggtgccg ttgatctggc cagcgaggta
     3541 gagacccggc agccgcttga cctccagggt cgggaacagc tcacgcggat cgacatagtc
     3601 gtactcgatc gcatagccga accggcggac ctcgacctgt tccaggccgg cgatggtgcg
     3661 caggaacagc agctgggtct cctcagagac cgaggtcgag acgccgttgg gatagaccgt
     3721 gtcgtcgtcc agcccctcgg gctcaaggaa gatctggtgg ctggtcttct cggcgaagcg
     3781 gacgaccttg tcctcgatcg aggggcagta gcgggggcca cgcccggtgg cccggccgcc
     3841 gtagacggcc gactccgtca ggcggtcggc gatgatccgg tgcgtttccg cattggtcca
     3901 ggtcacgccg cacgcgatct gcggcacggt gatgtcgcgg ttgaggaagg agaaggggac
     3961 cggcgtctcg tccgcggcct gcatctccag ccggtcccag gcaatggtcg aaccaaccag
     4021 ccgcgccgga gtgccggttt tcagtcggcc catgtcgagg cccaggctgt acagacggtc
     4081 ggccaggccg atggccggcg catcgccgac ccggccggcc ggaatgcgct tgtccccgag
     4141 gtggatcacg cccttgagga aggtgccggt ggtcagcacc accctcgggg cgctgtagat
     4201 cgcgccggac cccccgacca cgcctgtcag cacgccatcc tcgacgatca ggtcctcgac
     4261 cgcgtcggcg atgatctcga ggttgacctg tcccgccagc tcggcctgca tcgcctcgcg
     4321 atagagccgc cggtcgatct gcgaccgcgg cccccgcacc gcgggcccct tggagcggtt
     4381 gagcatccgg aactggatgc cggccttgtc ggccatgcgg cccatcaggc catccagcgc
     4441 gtcgatctcc cgcaccagat ggcccttgcc caggccgccg atggccgggt tgcagctcat
     4501 ctcgccgatg gtctcgagct tgtgcgtcag cagcagggtg cgcgccccga accgggccga
     4561 ggcggccgcg gcctcgcagc cggcatggcc gccgccgatc acgatcacat cccaggcg
//
LOCUS       DAORNX010000227         4602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13949_length_4602_cov_9.058940, whole genome shotgun sequence.
ACCESSION   DAORNX010000227 DAORNX010000000
VERSION     DAORNX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13949_length_4602_cov_9.058940"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="PLO65_10460"
     CDS             complement(<1..98)
                     /locus_tag="PLO65_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28708.1"
                     /translation="MPLVSDGYRRYALTLLLVIYTLNFLDRQVVNIL"
     gene            269..895
                     /locus_tag="PLO65_10465"
     CDS             269..895
                     /locus_tag="PLO65_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD28709.1"
                     /translation="MEIVIGTRKWSTWSLRPWLALKRTGAPFTETLVPLRELETSTAA
                     ILEHSPSGLVPVLKDGDLTVWDSLAICEYLAERFPEAKLWPADPADRALARAAAAEMH
                     SGFASLRGECPMDLTLVSSVDVSEATQANVRRLVDLWSGLLARSGGPFLLGGWSIADA
                     FFTPVATRFRSYGVRPSDYGDTGEAGRYVERLLETPEFQAWEKAALAE"
     gene            996..1919
                     /gene="msrP"
                     /locus_tag="PLO65_10470"
     CDS             996..1919
                     /gene="msrP"
                     /locus_tag="PLO65_10470"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRD28710.1"
                     /translation="MLIRHGGDLTENDVTPHALYLRRREFLGLGAAGMGLALAGEAAA
                     IPLKGGPSPLSTSETKTSKEDVTGYNNFYEFGVDKDDPARNAGGLKISPWTVKVDGEC
                     ARPGTVDLASFLRGNPLQERIYRHRCVEGWSMVIPWLGVPLADLLKRYQPTSKAKYVA
                     FETLYRPSEMPGQKRRVLDWPYREGLRIDEAMNPLTLMAVGLYGETLLNQNGAPMRLV
                     VPWKYGFKSIKSIVRISFTETQPRTAWNMSAPQEYGFYSNVNPEVDHPRWSQATERRI
                     GEFRRRPTLMFNGYGEQVASLYRGMDLKKFY"
     gene            1919..2515
                     /locus_tag="PLO65_10475"
     CDS             1919..2515
                     /locus_tag="PLO65_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HRD28711.1"
                     /translation="MSRKTVDAIALWAVSLACLLPLLWLGWRTYQGDLGANPIETLIR
                     QLGVWGLRMLIVGLTITPLARILKQPRLIRLRRPIGLFAFGYILLHLSTYIGVDQFFD
                     WQAIWKDIVKRPFITIGMAAFLLLIPLAATSFNAAIRRLGPLNWRRLHRLIYLIVPMG
                     VVHYYLLVKADHRPPLVYGAIVLLLLGWRAVVAFRARI"
     gene            complement(2512..3204)
                     /gene="radC"
                     /locus_tag="PLO65_10480"
     CDS             complement(2512..3204)
                     /gene="radC"
                     /locus_tag="PLO65_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRD28712.1"
                     /translation="MSQTGSTSQAHHLGHRERLRERALKSLDALPDYELLELFLFRTF
                     PRGEVKPLAKALLTRFGALGGVLTASPEDLKTVPGVGEAAALDLRLLGEATLRVGRET
                     IRRRTVIGSWSALLAYVKVALANEPREQFRVLFLDNKNQLIADEVMNRGTVDHAPVYP
                     REVVRRALELSASAVILVHNHPSGDPTPSRADIDMTRQVVEAGRSLKISVHDHLVVGR
                     EGVASFKALGLF"
     gene            3317..3601
                     /locus_tag="PLO65_10485"
     CDS             3317..3601
                     /locus_tag="PLO65_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRD28713.1"
                     /translation="MTGITYDPAKREATLGARGLDFDHALLVFDGPTVDSLDERFDYG
                     EIRRVTVGLLVGRMVIVVWTPRDDARHIISMRKANAREQRRFGHRFEEDR"
     gene            3585..3839
                     /locus_tag="PLO65_10490"
     CDS             3585..3839
                     /locus_tag="PLO65_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRD28714.1"
                     /translation="MKKIDETEIDEAAYAEIPELSDEWFEKAVPHRGGVPVRRGRPQS
                     ESPKKQVTLRLDADVLDTFRSSGPGWQGRLNAALRKAAGV"
     gene            complement(3836..>4602)
                     /gene="map"
                     /locus_tag="PLO65_10495"
     CDS             complement(3836..>4602)
                     /gene="map"
                     /locus_tag="PLO65_10495"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRD28715.1"
                     /translation="EGFEGMRKAGRLAAECLDMLIPHVVPGVTTGELDRLSREFVLDN
                     GGLPACLGYKGYMHTVCISTNHVVCHGIPGDRTLREGDIANIDVTVIVDGWHGDTSRM
                     YGVGEVSPRARRLVDVTYEGLARGLAVIRPGATFGDIGHAIQKYVESQRCSVVRDFCG
                     HGLGKVFHDSPNVLHFGRPGEGPVLREGMFFTVEPMVNLGRPQVKVLSDGWTAVTRDK
                     SLSAQCEHSCGVTADGVEIFTASPTGQFKPAIRGEG"
BASE COUNT          750 a         1511 c         1552 g          789 t
ORIGIN      
        1 aggatgttga ccacctgccg gtcgaggaag ttcagcgtgt agatgaccag cagcagggtc
       61 agggcgtagc gacggtagcc gtcgctgacc agcgggatgg cgtcaggcgg ggactcggga
      121 gcgacagact cctctgccgg cgcgaccgcg tcggtcatct gaacgctcct ccctgtcaaa
      181 cgcttgttct atttgttgcc ccaccttacg tgcgggtcag tctgtgttgg cagtcacgga
      241 tgcgtgatcg caaaatggga gaccgaaaat ggaaatcgtc atcggtacga ggaaatggtc
      301 gacctggtcg ttgcggccct ggctggcgct caagcgcacg ggcgcgccgt tcaccgagac
      361 gctggtcccg ttgcgcgagc tcgagacctc gaccgccgcg atcctcgagc attctccgtc
      421 gggcctggtg ccggtgctga aggacggtga cctgacggtg tgggactccc tggccatctg
      481 cgaatatctg gccgaacgct tccccgaggc gaagctttgg ccggccgacc cggccgaccg
      541 cgcgctggcg cgggccgcgg cggcggagat gcacagcggc ttcgcctcgt tgcgcgggga
      601 gtgcccgatg gatctgaccc tcgtgtccag cgtcgatgtc tccgaagcca cccaggccaa
      661 tgtgcgccgg ctggtggacc tttggtcggg cctgctggcg cggtcgggcg ggcccttcct
      721 gctcggcggc tggagcatcg ccgacgcctt cttcacgccc gtggcgacgc gcttccgcag
      781 ctatggcgtc aggcccagcg attacggcga cacgggcgag gcgggccgtt acgtcgagcg
      841 gctgctcgag acgcccgagt tccaggcctg ggagaaggcg gccctggcgg aatgatcacg
      901 ccccggcgcc cgtgagttga ctcgcgcgtt accgggaggc gtgatccacg ggcggcgcga
      961 agacgtagca ctgtgcgggg gcgcgggaga cgaccatgct gatccgtcac ggaggcgatc
     1021 tgaccgaaaa cgacgtcacg ccgcacgccc tctatctgcg acgccgcgaa ttcctgggcc
     1081 tcggcgcggc cggcatgggg ctggccctgg ccggcgaggc cgccgccatt cccctgaagg
     1141 gcggtccgag tcccttgtcg acgtccgaga cgaagacctc gaaggaggac gtcaccggct
     1201 acaacaattt ctacgaattc ggcgtcgaca aggacgaccc cgcccgcaac gccggaggcc
     1261 tgaagatcag cccctggacc gtgaaggtcg acggggagtg cgccaggccg ggcacagtcg
     1321 atctggccag cttcctgcgc ggcaacccgc tgcaggagcg gatctatcgt catcgctgcg
     1381 tcgagggctg gtcgatggtg atcccctggc tgggcgtgcc gctggccgat ctgctcaagc
     1441 gctaccagcc aaccagcaag gcgaagtacg tcgccttcga gaccctttac cggccgtccg
     1501 agatgccggg ccagaagcgc cgtgtgctcg actggccgta ccgcgagggc ctgcgcatcg
     1561 atgaggcgat gaacccgctg accctgatgg ccgtcggcct ctatggcgag accctgctga
     1621 accagaacgg cgcgccgatg cggctggtcg tgccgtggaa atacggcttc aagagcatca
     1681 agagcatcgt ccgcatcagc ttcaccgaga cccagccgcg cacggcctgg aacatgtcgg
     1741 cgccgcagga gtacggcttc tattcgaacg tgaaccctga ggtggaccac ccgcgttggt
     1801 cccaggcgac ggagcggcgc atcggcgagt tccgccgccg cccgacgctg atgttcaacg
     1861 gctatggcga acaggtggcc tcgctctacc gcggcatgga cctgaagaag ttctactgat
     1921 gagccgcaag accgtggacg cgatcgccct gtgggcggtc tcgctggcct gcctgctgcc
     1981 gctgctgtgg ctgggctggc ggacatacca gggcgatctg ggcgccaatc cgatcgagac
     2041 gcttatccgc cagctgggcg tctggggcct gcggatgctg atcgtcggcc tgaccatcac
     2101 gccgctggcc cggattctca aacagccgcg cctgatccgc ctgcgccggc cgatcggcct
     2161 gttcgccttc ggctacatcc tgcttcatct gtcgacctat atcggcgtgg accagttctt
     2221 cgactggcag gcgatctgga aggacatcgt caagcggccg ttcatcacca tcggcatggc
     2281 ggccttcctg ctgctgatcc cgctggccgc gacctcgttc aacgcggcga tccgccggct
     2341 cgggccgctc aactggcgcc ggctgcaccg gctgatctac ctgatcgtgc cgatgggggt
     2401 ggtccactat taccttctcg tgaaggccga ccaccgcccg ccgctggtct atggcgcgat
     2461 cgtgctgctg ctgctgggct ggcgggcagt cgtggccttc cgcgcaagga tctagaacag
     2521 tcccagcgcc ttgaagctgg ccacgccctc gcggccgacg acgaggtggt catggaccga
     2581 gatcttcagg ctgcggccgg cctcgaccac ctgtcgggtc atgtcgatgt cggcccggct
     2641 cggggtcggg tctccgctcg gatggttatg caccaggatc accgcgctgg ccgacagctc
     2701 cagcgcccgc cgcaccacct cgcgcggata gacgggcgcg tggtcgacgg tgccgcggtt
     2761 catcacctcg tcggcgatca gctggttctt gttgtcgaga aacaggacgc ggaactgctc
     2821 gcgcggctca ttggccaggg cgaccttcac ataggccagc aaggcgctcc aggagccgat
     2881 caccgtgcgc ctgcggatcg tctcgcggcc aacgcgcagg gtggcctctc caagcagccg
     2941 aaggtccaga gccgcggctt cgccgacgcc cgggacggtc ttcaggtcct ccggtgaggc
     3001 cgtcagcacg ccgcccagag ccccgaaccg cgtcagcagc gctttggcca gcggcttgac
     3061 ctcgccgcgc ggaaaggtgc ggaacaggaa cagctcgagc agctcatagt cgggcagggc
     3121 gtcgaggctc ttgagcgctc gctcgcgcag gcgttcgcga tggccgaggt ggtgagcctg
     3181 ggacgttgat ccggtctgtg acaagcgacc ccgcgcccct ggccctaacc tcccgagatc
     3241 atgcaatact cggatgagtt gtgcaaacgt cgtcaatcac gcaggattga cttccgattt
     3301 aatgtagtta cattaaatga cagggatcac ctacgatcct gccaagcgcg aggcgacgct
     3361 cggcgcgcgc ggcctggatt tcgatcatgc acttctggtg ttcgacggac caaccgtcga
     3421 ttcgctggat gagcgtttcg actatggtga gataagaagg gtcaccgtcg ggctgctggt
     3481 tggtcggatg gtgattgtcg tctggacgcc gcgcgatgat gcccgccaca tcatttctat
     3541 gaggaaggcc aatgccaggg agcagagacg tttcgggcat cgatttgaag aagatcgatg
     3601 agaccgaaat cgacgaggcg gcctacgctg aaattcccga attgtccgat gaatggttcg
     3661 agaaggcggt gccgcaccgt ggaggcgttc ccgtccgccg gggacgtccg caatcggagt
     3721 cgcccaagaa gcaggtgacc cttcgcctcg acgcggatgt gctcgatacc ttccggagca
     3781 gcggccctgg ctggcagggc cggctcaacg ccgccctgcg caaggccgcc ggcgtctagc
     3841 cttcgccccg gatcgccggc ttgaactggc cggtcgggga ggcggtgaag atctccacgc
     3901 cgtcggccgt cacgccgcag ctgtgctcgc actgggccga cagggacttg tcgcgggtca
     3961 cggcggtcca gccgtcgctg agcaccttca cctgcggccg gccgaggttc accatcggct
     4021 cgacggtgaa gaacatgccc tcgcgcaaga cggggccttc gcccggccgg ccgaagtgca
     4081 gcacgttggg actgtcgtgg aataccttgc ccaggccgtg gccgcagaag tcgcgcacca
     4141 cggagcagcg ctggctctcg acgtatttct ggatggcgtg gccaatgtcg ccgaacgtcg
     4201 cgcccggcct gatcacggcc aggccgcggg cgagaccctc gtaggtcaca tcgaccagac
     4261 ggcgcgcgcg cggcgagacc tcgcccacgc cgtacatgcg gctcgtgtcg ccatgccagc
     4321 cgtcgacaat cacggtcacg tcgatgttgg cgatgtcgcc ctcgcgcagc gtccggtcgc
     4381 cggggatgcc gtggcagacc acgtggttgg tcgagatgca cacggtgtgc atgtagccct
     4441 tgtagccaag gcaggcgggc aggccgccat tgtcgagcac gaactcgcgc gacagccggt
     4501 ccagttctcc cgtggtcacc cccggcacga cgtgcgggat cagcatgtcg aggcattcgg
     4561 cggccagccg gccggccttg cgcatgccct cgaagccctc gg
//
LOCUS       DAORNX010000228         4601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13955_length_4601_cov_6.128245, whole genome shotgun sequence.
ACCESSION   DAORNX010000228 DAORNX010000000
VERSION     DAORNX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13955_length_4601_cov_6.128245"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLO65_10500"
     CDS             <1..700
                     /locus_tag="PLO65_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRD28716.1"
                     /translation="LVAEDRLEVEADGKVRGRRLLKLDRLTVEDRRIDRPDPDMIARA
                     LLDEVRRKGLAILPMDAAAILRARLAFLGEPLTDTALIAGLEDWLAPLLKGRTSLATL
                     SAGQVADAIRGLVPWDVQRRLDAETPLRFTAPTGNSFAIDYAAEGGPKVEVRVGELYG
                     LSAHPTVAGTPLTFSLLSPGHKPIQTTKDIPGFWKGSWSDVRKDMRGRYPKHVWPEDP
                     AAAAPVTRAKLRGT"
     gene            960..2633
                     /locus_tag="PLO65_10505"
     CDS             960..2633
                     /locus_tag="PLO65_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD28717.1"
                     /translation="MAAAGGEGVWNRRGPARETPAGTQVLVNARILTIDSAFSTAEAL
                     AHRDGRIVAVGARADVMAQAGEEATILDCGGRTVLPGFIEPHMHFFPIAILGRYPDVG
                     PTVCVDAEAVIDRIGALSAAAPAGDWLVARQFDPSLQAGAERITRHTLDQVAPDRPVF
                     LFNASLHIGYCNSRALEIAGITAATADPPGAAYGRDLDGRPNGVLQGQATIFSVLGHN
                     MTAMVVDDVPEACLRVCAKANQVGVTTFCDQASGGFQGRREMAAFHAFAASGHMTTRL
                     RYSLIQGGHETWDEQGCRPGEGDAMARATGWKIVSDGSNQGRTGLQREPYFDGVGGTG
                     IAYVEPDALREIVVKRALQGWQVTVHANGDLAIDRALDAFDAAYAAGASRELRFRIEH
                     CSVLHDEQIARIADLGVSPSFLIGHVHYWGQAFRDEIFGPDKANLLCRAGSCGRAGIR
                     WTVHSDDTVTEMGPIRLIDNAVNRTLWREPGGVLNPAERVSVEQALIALTRDAAWQCH
                     SEHEIGTLEVGKFADFVVLDRDPLAIPPEEVGSVRVLETWVGGRQVYAA"
     gene            complement(2638..3426)
                     /locus_tag="PLO65_10510"
     CDS             complement(2638..3426)
                     /locus_tag="PLO65_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD28718.1"
                     /translation="MSRVVTSHTNPTVKAVRALHMRKAREETGRFLAEGLKIVTEAIA
                     LGFAPKILMFGQDGGDHPLLVKAIGATGMAGGDVIEVNREILEKVARRDNPQSVLAVF
                     EQKYTPVGSLEPRSAGVWVALHRVRDPGNLGTIIRTADAAGCGGVILVGECCDPWSVE
                     AVRATMGSIFAVPIVKMTEAEFIAWRGGWPGSVVGTLLTATVDHRAADYQRPTLILMG
                     NEQAGLTPELAALCDVNVKIPMRGRADSLNLAVATGIMIYAAGD"
     gene            complement(3423..>4244)
                     /locus_tag="PLO65_10515"
     CDS             complement(3423..>4244)
                     /locus_tag="PLO65_10515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD28719.1"
                     /translation="VDSGGGRKLERYGPYHVVRPEPQCLWTPRLPAARWEAADAVFDP
                     TDEDEAGRWRFKGPPVDSWELGWGDVKFLGRFTNFRHLAFFPEQAANWAWLDQRQRAA
                     GRPLKMLNLFGYTGVASLVAAAAGAQVTHVDASKQSVQRARENAALSGIETVRWIVED
                     ARRFVQREDRRGNRYDGIILDPPKFGRGPDGEVWRLFEDLPEMVRLCAGLLSDDASFL
                     ILNAYAARISGASMAGLVAEATANRAGRIDWGELALVETAGDRQVGLSFFARWSA"
     assembly_gap    4245..4288
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4320..>4601)
                     /locus_tag="PLO65_10520"
     CDS             complement(4320..>4601)
                     /locus_tag="PLO65_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28720.1"
                     /translation="LVTGDVTLLSAPDGSLLAVGWACATVAGVVGLRRARAPLRLRDM
                     LFLPLYWPLQSLAAAHAVTQLLHRPFHWDKTPHAARTGQGAARAGIARG"
BASE COUNT          659 a         1614 c         1613 g          671 t
ORIGIN      
        1 gctcgtcgcc gaggaccggc tggaggtcga ggccgacggc aaggtgcgcg ggcgtcggct
       61 gttgaagctc gaccgattga ccgtcgaaga tcggcggatc gacaggcccg acccggacat
      121 gatcgcccgg gccctgctcg acgaggtgcg ccgcaagggc ctcgccatcc tgccgatgga
      181 cgccgccgca atcctccgcg cgcggctggc cttcctcggc gagcctctga ccgacacggc
      241 gctgatcgcc ggactggagg actggctcgc tccgttgctc aagggaagga ccagcctggc
      301 cacgctatcg gccggccagg tcgccgacgc catccgcggc ctggtcccct gggatgtcca
      361 gcgtcgcctc gacgccgaga cgccgctgcg cttcaccgcc ccgaccggca acagcttcgc
      421 gatcgactac gccgccgagg gcgggccgaa ggtcgaggtc cgggtcggcg agctctacgg
      481 cctgtcggcg catccgacgg tcgccggaac gccgctgacc ttcagcctgc tgtcgcccgg
      541 tcacaagccg atccagacga cgaaggatat tcccggcttc tggaaggggt cctggtccga
      601 tgtccgcaag gacatgcgcg gtcgctatcc caaacatgtc tggcccgagg acccggccgc
      661 ggcggcgccg gtcacccgcg ccaagctgag ggggacatag gggacaccgc ccctcggtcc
      721 ctgtctcctg tcactgcgga acctgatctg ggtgcacgcg gcgctggcgc cggtcgccgc
      781 tcgtggccat ggtgcgcccg ttcgatcggg aggatctggt ggacagtggt gtgagggagc
      841 ggtcgccggg ctcgcgcggc ctgtcgtatc gaccctttca ggtccgggat ccacgtctgc
      901 atggctgtcc ctgttgcgcg cccatcctca gggaccttgg ccagctgtcg ctgcccttca
      961 tggccgcggc gggcggcgaa ggcgtctgga accgacgggg ccccgcgcgc gagactcccg
     1021 cggggacgca ggtgctggtc aacgcgagga tcctgaccat cgacagcgcc ttttcaaccg
     1081 ccgaggccct ggcgcaccgc gacgggcgga tcgtcgccgt gggcgcgcgc gccgacgtca
     1141 tggcgcaggc cggagaggag gcgacgatcc tcgactgcgg cggtcgaacg gtcctgccgg
     1201 ggttcatcga gccccacatg cacttcttcc cgatcgccat cctcggccgc tatcccgacg
     1261 tcggccccac cgtctgcgtc gacgccgagg cggtgatcga ccggatcggc gcgctgtcgg
     1321 ccgcggcgcc ggcgggggac tggcttgtcg cgcgccagtt cgatccgtcc ctgcaggcgg
     1381 gcgccgaacg gatcacgcgt cacaccctcg accaggtcgc gcctgaccgg ccggtgttcc
     1441 tgttcaacgc ttccctgcac atcggctact gcaacagtcg cgctctggag atcgccggca
     1501 tcacggccgc aacggccgat ccgccgggcg cggcctatgg ccgcgacctc gacggccgtc
     1561 cgaacggggt gctgcagggc caggcgacga ttttcagcgt attgggccac aacatgacgg
     1621 ccatggtggt cgatgacgtg cccgaggctt gcctgcgcgt ctgcgccaag gccaaccagg
     1681 tcggggtgac gaccttttgc gaccaggcct ccgggggctt tcaggggcga cgggaaatgg
     1741 ccgccttcca cgccttcgcc gcctcgggcc acatgacgac ccgcctgcgc tactcattga
     1801 tccagggcgg ccacgagacc tgggacgaac agggttgccg gccgggggaa ggcgacgcca
     1861 tggcccgcgc caccggctgg aagatcgttt ccgacgggtc caaccagggc cgcacggggc
     1921 tgcagcgcga gccctacttc gacggcgtcg ggggaaccgg catcgcctac gtcgagcccg
     1981 acgccctgcg cgaaatcgtc gtcaaacgcg ccctgcaggg ctggcaggtc acggtccacg
     2041 ccaatggcga cctggccatc gaccgggcgc tggacgcctt tgacgccgcc tacgccgccg
     2101 gggcttcgcg ggagctgcgt ttccgcatcg agcattgttc ggtgctgcac gatgagcaga
     2161 ttgcccgcat cgccgacctg ggcgtcagcc ccagcttcct gatcggtcac gtccactact
     2221 ggggccaggc attccgcgac gagatcttcg ggccggacaa ggccaacctg ctgtgccgcg
     2281 ccggctcctg cggccgggcc gggatccgct ggacggtcca ctccgacgac acggtcaccg
     2341 agatgggacc catcaggctg atcgacaacg cggtgaaccg gaccctgtgg cgcgagccgg
     2401 gcggcgtgtt gaatccggcc gagcgggtca gcgtcgaaca ggcgctcatc gccctcaccc
     2461 gcgacgcggc ctggcagtgc cattcggaac acgagatcgg cacactggag gtcggcaagt
     2521 tcgcggactt cgtcgtgctg gaccgcgatc ccctcgccat tcctccggag gaggtcggct
     2581 cggtgcgggt gctggagacc tgggtcggcg gccggcaggt ctacgcggcg tgaggcttca
     2641 gtcccccgcc gcgtagatca tgataccggt cgccaccgcc aggttcaggc tgtcggctcg
     2701 gccgcgcatg gggatcttga cgttgacgtc gcacagcgcc gccagctccg gcgtcaggcc
     2761 ggcctgttcg ttgcccatca ggatcagcgt cggccgctgg tagtcggccg cgcggtggtc
     2821 gaccgtcgcg gtcagcaggg tgccgaccac cgagccgggc cagccgccgc gccaggcgat
     2881 gaactcggcc tcggtcatct tcacgatcgg cacggcgaag atcgagccca tggtcgcgcg
     2941 caccgcctcg accgaccagg gatcgcagca ctcgccaacc aggatcaccc cgccgcagcc
     3001 ggccgcgtcg gccgtgcgga tgatggttcc aaggttgccg ggatcgcgga ccctgtgcag
     3061 cgcgacccag acgcccgccg atcgcggttc cagcgacccg acgggggtgt acttctgctc
     3121 gaacaccgcc aggacggact ggggattgtc ccgtcgggcg accttctcga gaatttcgcg
     3181 gttgacctcg atgacgtcgc cgccggccat gccggtcgcg ccgatggcct tgaccagcag
     3241 cgggtggtcg ccgccgtcct ggccgaacat caggatcttc ggcgcgaagc ccagcgcgat
     3301 cgcctcggtg acgatcttga ggccttcggc gaggaaccgg ccggtctcct cgcgggcctt
     3361 gcgcatatgc agcgcccgga ccgccttgac cgtcgggttg gtgtgggagg tgacgacccg
     3421 gctcatgccg accaccgggc gaagaaggag aggcccacct gccggtcgcc ggccgtctcg
     3481 accagcgcca gctcgcccca gtcgatgcgg ccggcgcgat tggctgtcgc ttcggccacc
     3541 agcccggcca tcgaggcccc cgagatgcgc gcggcgtagg cgttcaggat caggaacgag
     3601 gcgtcgtccg agaggaggcc cgcgcacagc cggaccatct ccggcaggtc ctcgaacagt
     3661 cgccagacct cgccgtcggg cccgcgcccg aacttgggcg gatcgagaat gatcccgtcg
     3721 tagcggttgc cccggcgatc ctcgcgctgg acgaagcggc gggcgtcctc gacgatccag
     3781 cgcacggtct cgatgccgga cagggcggcg ttctcgcgcg cccgctgtac cgactgcttg
     3841 ctggcgtcga cgtgggtgac ctgggcgccg gccgccgccg cgaccagact ggcgacgccg
     3901 gtgtagccga acaggttgag catcttcagc ggccggccgg cggcgcgctg gcgctggtcg
     3961 agccaggccc agttggccgc ctgctcgggg aaaaaggcca ggtggcggaa gttggtgaaa
     4021 cggccgagaa acttcacatc gccccagccg agctcccagc tgtcgaccgg cggccccttg
     4081 aaccgccagc ggccggcttc gtcctcgtcg gtcgggtcga acaccgcgtc ggcggcttcc
     4141 cagcgggcgg cgggcaggcg cggggtccac aggcactgcg gctcgggccg gaccacgtga
     4201 taggggccgt agcgctcgag cttgcggccg ccgccgctgt cgacnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cgcgggcgtt tcggcgatgg tcgggccggt
     4321 catccccggg cgataccggc tcgggcggcg ccctgtccag tgcgggccgc gtgcggggtc
     4381 ttgtcccagt gaaacggacg atggagcaac tgcgtcaccg catgggccgc cgccagcgac
     4441 tggagcggcc agtagagtgg caggaagagc atgtcgcgca gccgcagcgg cgccctggcg
     4501 cggcgcaggc cgacgacacc ggcgaccgtc gcgcaggccc agccgaccgc cagcaatgat
     4561 ccgtcgggcg cgctgagcag ggtgacgtcg ccggtgacca g
//
LOCUS       DAORNX010000229         4588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_13993_length_4588_cov_6.661813, whole genome shotgun sequence.
ACCESSION   DAORNX010000229 DAORNX010000000
VERSION     DAORNX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13993_length_4588_cov_6.661813"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLO65_10525"
     CDS             <1..483
                     /locus_tag="PLO65_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HRD28721.1"
                     /translation="DPLALVGLAAAAAVAAGALPEREPHPGAYHAFDALSGAFAISDI
                     WPAVFVRFEAGLLQELGFGLDLSRCASTGATDDLVWVSPRTGRAVSRAAGEPYADRLL
                     PLPGFLLGSQGGLAEGDIRAGFDLTGHFLAQFVFGPLNRPLPPARVWLLERLADAGML
                     "
     gene            561..1718
                     /locus_tag="PLO65_10530"
     CDS             561..1718
                     /locus_tag="PLO65_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRD28722.1"
                     /translation="MPSPAPIALAERQTRLAALRTLMDAAGVQAVLLGATASLRYFTG
                     LVWHASERLCGAIVHLDGRLEYVCPAFEREKVAGIVGVPGEIITWEEHENPYALVADR
                     LGPAGRLAVDDQIALFMFLGLRKVLGDERLADAGPLIHRLRRSKSPAEIALMQRAKAI
                     TIEVHRRAWASLTPGVRASEVTRYIDQQHRALNGGTGSSFCIVSFGEDTALPHGGETD
                     RALAPGDMVLIDTGTTLDGYNSDITRSYVFGEATPRVRRIWDIEKEAQAAAFAAATLG
                     RPCENVDDAARAVLVRHGLGPDYKLPGTPHRTGHGIGLDIHESPNLVRGDATPLEVGM
                     CFSNEPMIVVPGAFGVRLEDHFHMTADGPAWFTKPQFSLDDPFGDVGHFEP"
     gene            1912..4146
                     /gene="parC"
                     /locus_tag="PLO65_10535"
     CDS             1912..4146
                     /gene="parC"
                     /locus_tag="PLO65_10535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRD28723.1"
                     /translation="MTLHTPPPGDGDRILEEPLTEALSRRYLAYALSTISSRALPDVR
                     DGLKPVHRRLMYAMSNMRLSPEAAARKCAKVVGEVMGNFHPHGDQSIYDALVRLAQDF
                     AQRYQLVDGQGNFGNIDGDNAAAMRYTECKMTPAAMLLLDGIDEDAVDFRPTYDGQDE
                     EPVVLPSGFPNLLANGSSGIAVGMATSIPPHNAAELIDACLLLLKRPEATTQDLMGHV
                     LGPDFPTGGVIVEPAASLLETYETGRGGVRVRARWEREDQARGGWQIVVTEIPYQVKK
                     AELIEALADLIESKKAPLLGDVRDESAEDIRLVLEPKSRNVEPEVLMESLFRLSDLET
                     RFPVNMNVLDARGTPGVMGLKQALLAFLAHRREVLVRRARFRLGKIEARLHVLDGLLI
                     AFLNLDEVIRIVRYEDEPKQKLIAAFALTDIQADAILNTRLRQLAKLEEMELRREHAE
                     LAEERDGIVAMLADEKLQWKLVGEGLKDVRKVLGPDTTVGRRRSELAEAPAVSIEASV
                     EAFVVREPITVILSDKGWIRAAKGRIEDPSELKFKEGDKLGFIVPAETTDKLLIFASD
                     GRFFTLDCSKLPGARGHGEPLRLMVDIDDRVKIIDVFPHRPGRKRVLASKAGYGFILP
                     EEEALAFRKAGKQVLNGEALVSLEAAGDRLAVIGDNGKILVFPLDELPEMPRGKGVKL
                     QSYREGGLRDALVFAAATGAAWTDAAGRTRDWPEWKDWEGRRSAAGKLAPRGFPTSKR
                     FRPK"
     assembly_gap    4328..4370
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          698 a         1631 c         1542 g          674 t
ORIGIN      
        1 gacccgctgg cgctggtggg cctcgccgcc gccgccgccg tggccgccgg ggccctgccc
       61 gagcgcgagc cgcatccggg ggcctaccac gcgttcgacg ccctttccgg cgccttcgcg
      121 atctccgaca tctggccggc cgtgttcgtg cgcttcgaag ccggcctgct gcaggagctg
      181 ggcttcggcc tcgatctcag cagatgcgcc tcgaccggcg caactgacga tctggtctgg
      241 gtcagtccgc gcacggggcg ggccgtcagc cgcgccgcgg gcgaacccta cgccgaccgt
      301 ctgctgccgc tgccgggctt cctgctcggc tcgcaaggcg gcctggccga gggtgatatc
      361 cgcgccgggt tcgacctgac cggccatttc ctcgcccagt tcgtgttcgg cccgctcaac
      421 cgccccttgc cgccggcccg tgtctggctg ctcgagcggc tggccgacgc gggcatgctg
      481 tagcgagtcc gccggtcccc gccttcgcgg ggacaatcgg tgagagatgc cgctagactg
      541 cccgccaagg agtccccgcc atgccaagtc ccgccccgat cgccctggcc gagcgccaga
      601 cccgtctcgc cgccctgcgc accctgatgg acgccgccgg ggtccaggcc gtgctgctgg
      661 gggccacagc cagcctgcgg tacttcaccg gcctggtctg gcacgcctcg gagcggctgt
      721 gcggcgccat cgtccacctc gacggccgcc tcgagtacgt ctgcccggcc ttcgagcgcg
      781 agaaggtcgc cggcatcgtc ggcgtcccgg gcgagatcat cacctgggag gagcacgaaa
      841 atccctacgc actcgtcgcc gaccgcctcg gtcccgccgg acggctggcc gtcgacgacc
      901 agatcgccct gttcatgttc cttggcctgc gcaaggttct cggtgatgag cggctggccg
      961 acgccgggcc gttgattcat cgcctgcgcc gcagcaagtc gccggccgag atcgccctga
     1021 tgcaacgggc caaggccatc accatcgagg ttcaccgccg cgcctgggcg agtctgacgc
     1081 cgggcgtgcg ggcgtcggag gtgacccgct acatcgacca gcagcaccgc gccctcaacg
     1141 gcgggaccgg gtccagcttc tgcatcgtct cgttcggcga ggacaccgcc ctgccgcacg
     1201 gcggggagac cgaccgggcg ctggccccgg gtgacatggt gctgatcgac accggcacga
     1261 cgctcgacgg ctacaacagc gacatcaccc gctcctacgt gttcggcgag gcgacgccgc
     1321 gcgtgcggcg catctgggac atcgagaagg aagcccaggc cgccgccttc gccgccgcca
     1381 ccctcggccg gccctgcgag aatgtcgacg acgccgcccg cgccgtcctc gttcgacacg
     1441 ggctgggccc cgactacaag ctgcccggca cgccgcaccg gacgggccac ggcatcggcc
     1501 tggacatcca cgagtcgccc aacctcgtgc gcggcgacgc gacgccgctg gaggtcggca
     1561 tgtgcttctc caacgagccg atgatcgtcg tgccgggcgc cttcggcgtg cggctggagg
     1621 accacttcca catgaccgcc gacgggccgg cgtggttcac caaaccgcag ttcagtctgg
     1681 acgacccgtt cggggatgtg gggcatttcg agccgtagac ttacccctct cccggcggga
     1741 gagggatggg agggtgaggg gctacggttt ggcccggccg gcgccgtaaa ccctcatccg
     1801 acctgctccg caggccacct tctccctgcg ggagaaggaa gcaatcgacg cggcgacaac
     1861 gcccccgccg ccacgctaaa catcagaacc acctgagttt ccgattcgag catgaccctc
     1921 cacacacccc cgcccggtga cggcgaccgc attcttgaag agccgctgac cgaggcgctg
     1981 tcgcggcgct atctggccta cgccctgtcg accatcagct cgcgggcgct gcccgacgtg
     2041 cgcgacgggc tcaagcccgt gcaccggcgg ctgatgtacg ccatgagcaa catgcggctg
     2101 agccccgagg ccgcggcgcg caaatgcgcc aaggtggtcg gggaggtgat gggcaacttc
     2161 cacccgcacg gcgaccagtc gatctatgac gccctggttc gtctggccca ggacttcgcc
     2221 cagcgctacc agctggtcga cggccagggg aacttcggca acatcgacgg cgataacgcc
     2281 gcggccatgc gctacaccga gtgcaagatg acgccggcgg ccatgctgct gctggacgga
     2341 atcgacgagg acgcggtcga cttcaggccg acctacgacg gacaggacga ggagccggtc
     2401 gttctgccct cgggctttcc caacctgctg gccaacggca gctcgggcat cgccgtgggc
     2461 atggccacct cgatcccgcc gcacaacgcc gccgagctga tcgacgcctg cctgctgttg
     2521 ctcaagcggc ccgaggcgac cacgcaggac ctgatgggcc acgtgctggg ccccgatttc
     2581 ccgaccggcg gcgtaatcgt cgagccggcg gcctcgctgc tggagaccta cgagaccggc
     2641 cgcggtggcg tgcgggtgcg cgcgcgctgg gaaagggaag accaggcccg tggcggctgg
     2701 cagatcgtcg tcaccgagat cccctaccag gtcaagaagg ccgagctgat cgaggccctg
     2761 gccgacctga tcgaaagcaa gaaggccccg ctgctcggcg acgtgcgcga cgaaagcgcc
     2821 gaggatatcc gcctggtgct cgagccgaag agccgcaacg tcgagcccga ggtcctgatg
     2881 gagagcctgt tccggctctc ggacctcgag actcgtttcc cggtcaacat gaatgtgctc
     2941 gacgcccggg gcacgcccgg cgtcatgggc ctgaagcagg cgctgctggc cttcctcgcc
     3001 caccgccgcg aggtgctggt gcgccgggcg cgcttccggc tgggcaagat cgaggcccgg
     3061 ctccacgttc tcgacggcct cttgatcgcc ttcctcaatc tcgacgaggt gatccgcatc
     3121 gtccgctacg aggacgagcc gaagcagaag ctgatcgccg ccttcgccct gaccgacatc
     3181 caggccgacg ccatcctcaa cacccgcctg cgccagctgg cgaaactgga agagatggag
     3241 ctgcgccgcg agcatgccga gctggccgag gagcgcgacg gcatcgtcgc catgctggcc
     3301 gatgagaagc tgcagtggaa gctggtcggc gaggggctca aggacgtccg caaggtgctc
     3361 ggccccgaca ccacggtcgg ccggcgccgc tccgagctcg cggaggcgcc ggcggtgtcg
     3421 atcgaggcct cggtcgaggc cttcgtggtg cgcgaaccga tcaccgtcat cctgtcggac
     3481 aagggctgga tccgcgccgc caagggccgc atcgaggatc catccgaact gaagttcaag
     3541 gaaggcgaca agctgggctt cattgttcca gcggaaacaa cggacaagct gctgatcttc
     3601 gcgtccgatg gccggttctt caccctcgat tgcagcaagc tgcctggcgc ccgcggccac
     3661 ggcgagccgc tgcggctgat ggtcgacatc gacgaccggg tgaagatcat cgacgtcttc
     3721 ccgcaccggc cgggccgcaa gcgcgtgctg gccagcaagg ccggctacgg cttcatcctg
     3781 cccgaggagg aggcgctggc cttccgcaag gcgggcaagc aggtgctcaa cggcgaggcg
     3841 ctggtgtcgc tggaagcggc cggcgaccgt ctggcggtga tcggcgacaa cggcaagatc
     3901 ctcgtctttc cgctcgacga actgccggag atgccgcgcg gcaagggcgt caagctgcag
     3961 agctatcgcg agggcgggct gcgcgacgcc ctggtgttcg ccgcggcgac aggggcggcc
     4021 tggaccgacg cggccggccg cacccgcgac tggcccgaat ggaaggactg ggaaggccgt
     4081 cgcagcgccg ccggcaagct ggccccgcgc ggcttcccga ccagcaagcg gttccggccg
     4141 aagtagcggc gttcctcccc gcgtggcggg gagggggacc acgcggagcg cggtggaggc
     4201 gccggcgctc ctgtgcgcgg caattgcctg aagtccgctc ttggctcttc ctctccccca
     4261 gggggaggtg gcccggcgga gccgggtcgg agagggagga gccgctgttt gacgcgttct
     4321 tcccgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctgctccgc
     4381 atggtccccc tctccctccg ggagaggatg tccgagggcc ttattgattt cccgctatcc
     4441 aactgcctga atttccccgc atctgtcgcc gatgtccgac cggacaaaac aatctgtcca
     4501 tcaccccgtg aacctgcccc catactcgcg tcatccccgc cgcacgggga gggcgcacgg
     4561 ccagcgacct tgcggcggcg gggatgga
//
LOCUS       DAORNX010000230         4569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14064_length_4569_cov_8.877492, whole genome shotgun sequence.
ACCESSION   DAORNX010000230 DAORNX010000000
VERSION     DAORNX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14064_length_4569_cov_8.877492"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /gene="fliF"
                     /locus_tag="PLO65_10540"
     CDS             complement(<1..1424)
                     /gene="fliF"
                     /locus_tag="PLO65_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRD28724.1"
                     /translation="MSALRGFGLGRLAALAGIGVGLAAALVALTMNIGTQPKALLYSN
                     LDLKEASAVVQALEQAGVKYEAQGDGSTILVPRDQVASTRLMIASKGLVSSGSIGYEI
                     FDQGATLGQTDFVQQLNRQRALEGELARTIHSLDGVTSVRVHLNLPRRQLFEEEAQPA
                     SASVTIGIGGRKPSEDQVQAVQNLVAGAVPGLKAEQVAVIDQHGKTLSAGGDGSMAGK
                     MADDRKSEVERRLAARIKELVEGVVGPGRARVEVTADIDMARVTEQKETFDPDGQVVR
                     SEQTGEEQASENEPDSANTVSAAGNIPGAAGGAAGGLTSSRTGRTDSTTNYEISKTTT
                     TEVREPGTVKKISVAVAVDGVTAVGKDGKPGAYTPRSEAEMQRLDELVKAAVGFDSER
                     GDTVRVTNVRFAREAMDLDGVTAKGGLLSGFDKNDIMRAAELGVLAILGILLTLFGVR
                     PLLKGLTNGGGQRLPALAGAAGGGA"
     gene            complement(1623..1904)
                     /locus_tag="PLO65_10545"
     CDS             complement(1623..1904)
                     /locus_tag="PLO65_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HRD28725.1"
                     /translation="MLQQQRVNNRGEKYVIGPTGAPLTLSDLPPANTERWVIRRKAEV
                     VAAVRGGLLSLDEACDRYRLTNEEFLTWQKSIDRHGLAGLRTTRLQQYR"
     gene            complement(2070..3476)
                     /locus_tag="PLO65_10550"
     CDS             complement(2070..3476)
                     /locus_tag="PLO65_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRD28726.1"
                     /translation="MSINSAMLAGVTGLVSNSSALAAISDNIANVNTVGYKRSQANFQ
                     TLVTSRAAGASYAAGGVTAQTRRFITTQGLPTQTTSATDLSISGQGFFVATEKAEDLQ
                     ASDVRSFTRAGSFQLDNLGYLRNDAGLYLQGWLVDDEGVITTDPSDITRLKSINVSSV
                     GGAAEQTTRATVNANLQSSQALSADVASYNAATNSMAMYDPDAGTGVRPDFSIQVPVS
                     DSKGGKRTVQVDFLKSANPNEWYAEVRAVPASDVETGTGLTNGQLATGLVVFTPDGRL
                     DSTATTLFPDPANPILTFGASADAAPGAGDFKWAGSLGVGAQEIRLDLGGAAGGLTQF
                     DSQSLVQSVATNGTAFGNLTNIEIDEQGFVTAIFDNGISRQIAQVAIATFPNADGLIP
                     VSGNAYRVSQQSGTYNFKTPGSGGAGFISPSTLEASTVDLSSEFTGLITTQRAYSASS
                     KIITTADQMLEELLNIKR"
     gene            complement(3509..4186)
                     /locus_tag="PLO65_10555"
     CDS             complement(3509..4186)
                     /locus_tag="PLO65_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRD28727.1"
                     /translation="MTTTIDPTATAANASVNEKLSSSRNRLADSEQTFLALLTTQLKN
                     QDPLSPLDSNEFTAQIVQMTGVEQQLMTNDLLKMLVGMSDGGLAGSVDLIGKTVTAAS
                     DEAVMTDGAASWTYDLDRNASSVKYEVLNSQGTTVWSRTDAAVAGGEHTLDWDGVTTD
                     GQNLPDGGTYTLRVTATGSGGETINATPRLTGVATAVQTIDGQTVVSIGKLKVPVSSI
                     TGVQSTT"
     gene            complement(4195..>4569)
                     /locus_tag="PLO65_10560"
     CDS             complement(4195..>4569)
                     /locus_tag="PLO65_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRD28728.1"
                     /translation="DLQLDPHGLGKVDVAIEIDRNGKLTAALSFDSPQAAADLRGRAG
                     ELRQALEQAGFDVSENSLTFDAAGQGAGSGGREAGQQQDRAWNGRAFQRVQSSLDDAD
                     LTLATVPANPTRRTRSGVDIRI"
BASE COUNT          641 a         1471 c         1642 g          815 t
ORIGIN      
        1 gcgccgccgc cggcggcccc ggccagggcc ggcagacgct ggccgccgcc gttggtcagg
       61 cccttgagca acggccgcac tccgaacagc gtcagcagga tgccgaggat ggccaggacg
      121 cccagctcgg ccgcccgcat gatgtcgttc ttgtcgaagc cgctcagcag gccgcccttg
      181 gccgtgacgc cgtccaggtc catcgcctcg cgggcgaagc ggacattggt cacccgcacg
      241 gtgtcgccgc gttcggagtc gaagccgacg gcggccttca ccagctcgtc gaggcgttgc
      301 atctcggcct cggagcgcgg cgtgtaggcg ccgggcttgc cgtccttgcc gacggcggtg
      361 acgccatcga cggcgacggc gaccgagatc ttcttgaccg tgcccggctc gcgcacctcg
      421 gtggtggtgg tcttgctgat ctcgtagttg gttgtggaat cggtccggcc cgtgcgggac
      481 gacgtcaggc cgcccgcggc cccccccgcc gcgcccggga tgttgcccgc ggccgagact
      541 gtgttggccg agtcgggctc gttctcggag gcctgctcct cgccggtctg ttcggagcgg
      601 acgacctggc cgtcgggatc gaacgtttcc ttctgctcgg tgacgcgggc catgtcgatg
      661 tcggcggtga cctcgacgcg cgcgcggccc gggccgacga cgccctcgac cagttccttg
      721 atgcgggcag cgaggcggcg ctcgacctcg ctcttgcggt cgtcggccat cttgccggcc
      781 atcgagccgt cgccgccggc ggacagcgtc ttgccatgct ggtcgatcac cgcgacctgc
      841 tcggccttga ggcccggcac ggccccggcg acgagattct ggaccgcctg gacctggtcc
      901 tcggacggct tgcggccgcc gatgccgatc gtcaccgagg ccgaggccgg ctgggcctcc
      961 tcctcgaaga gctgccggcg cggcaggttc aggtgcaccc ggaccgaggt cacgccgtcg
     1021 aggctgtgga tggtccgcgc cagctcgccc tcaagcgccc gctggcggtt cagctgctgg
     1081 acgaagtcgg tctgtccgag ggtcgcgccc tggtcgaaga tttcgtagcc gatcgagccc
     1141 gagctcacga ggcccttgct ggcgatcatc agccgggtcg aggcgacctg gtcgcgcggc
     1201 accaggatgg tcgagccgtc cccctgggcc tcgtacttca cgccggcctg ctcaagcgcc
     1261 tggacgacgg ccgaggcttc cttgagatcg aggtttgaat agagcagcgc cttgggctgc
     1321 gtcccgatgt tcattgtcag ggccacgagg gcggcggcaa gcccgacccc gatgccggcc
     1381 agcgccgcga gacggccgag gccgaaccca cgcaaggcgc tcatgaactg ttccaaccga
     1441 gcctgccccc aacgcattgg accaacggcg ccgacaggac cggaccgcta ggcaggaatt
     1501 accgggtgcg tggtaaacgg ggccttaagg tccgcgccgc cgcccctgaa cgacgacggc
     1561 cgcgcccggt ctggacgcgg ccgcctttac gcgagccatt ctggaagggg gggaggaagg
     1621 gctcagcgat actgttgcag tcgggtcgtc cgcaggccgg cgaggccatg gcggtcgatg
     1681 gacttctgcc aggtcaggaa ttcctcgttg gtcagcctgt agcgatcaca ggcctcatcg
     1741 aggctgagca agcccccgcg caccgccgcc acgacctccg ccttgcgtcg gatcacccaa
     1801 cgctcggtgt tggccggtgg caggtcggac agcgtcagcg gcgcaccggt cggaccgatc
     1861 acgtactttt cgcccctgtt gttcacgcgc tgctgttgaa gcatggctct aggcctctct
     1921 caccatcact gttgaggaga accgtacgcc agcggcgata acggcgcgtg aatcgcgata
     1981 gtaaaaacgc gggtaaccac tatcgctccc aagttaattt cggtgtttca tcctgaaaca
     2041 ctcgaattaa aacaagctaa acttcaagtc taacgcttga tgttcagcag ctcttccagc
     2101 atctgatcgg ccgtggtgat gatctttgac gaggcggagt aggcccgttg cgtggtgatc
     2161 aggcccgtga actcgctgga gaggtcgacg gtcgaggctt caagcgtcga cggcgagatg
     2221 aagcccgccc cgccgctgcc gggggtcttg aagttgtagg ttccgctctg ctggctgacc
     2281 cggtaggcgt tgccgctgac cgggatcagg ccgtcggcgt tggggaaggt ggcgatggcc
     2341 acctgggcga tctggcgcga gatgccgttg tcgaagatgg cggtgacgaa gccctgttcg
     2401 tcgatctcga tgttggtcag gttgccgaag gcggtgccgt tggtggccac ggactggacc
     2461 agcgactggc tgtcgaactg ggtcaggccg cccgccgcgc cgccgagatc gaggcggatc
     2521 tcctgggcgc cgacgccaag gctgccggcc catttgaagt cgcccgcgcc gggcgcggcg
     2581 tcggccgagg cgccgaaggt caggatcggg ttggccgggt ccgggaagag ggtggtggcc
     2641 gtgctgtcga gtcggccatc gggggtgaac accaccaggc cggtggccag ctgaccgttg
     2701 gtcaggccgg ttccggtttc gacgtcgctg gccgggacgg cccgcacttc ggcgtaccat
     2761 tcgttgggat tggcgctctt gaggaagtcg acctggaccg tccgcttgcc gcccttggag
     2821 tcggacaccg gaacctggat cgagaagtcc gggcggaccc ccgtcccggc gtcggggtcg
     2881 tacatcgcca tcgaattggt cgcggcgttg taggaggcga cgtcggccga cagcgcctgg
     2941 ctcgactgca ggttggcgtt gaccgtggcc cgcgtggtct gttcggccgc gccgccgacc
     3001 gacgagacgt tgatcgactt cagccgggtg atgtccgacg gatcggtggt aatgacccct
     3061 tcgtcatcga ccagccagcc ctgcaggtac agcccggcgt cgttgcgcag gtagcccagg
     3121 ttgtcgagct ggaacgagcc ggcgcgggtg aacgagcgga catcgctggc ctgcaggtcc
     3181 tcggccttct cggtggcgac gaagaagccc tgccccgaga tcgacaggtc ggtcgccgag
     3241 gtggtctggg tgggcaggcc ctgggtggtg atgaagcggc gggtctgggc ggtgacgccc
     3301 ccggccgcgt acgaggcgcc ggccgcgcgc gaggtcacca gggtctggaa attggcctgg
     3361 cttcgcttgt agccgacggt gttgacgttg gcgatgttgt cggaaatcgc cgcgagggcg
     3421 gacgagttcg acaccaggcc ggtcacgccg gcgagcatgg cgctgttgat gctcatggga
     3481 ggctatcctt ccttgtggct ggccaggatc acgtcgtgga ctgcacgccg gtgatcgaag
     3541 acacggggac tttgagtttg ccgatgctga cgacggtctg accgtcgatg gtctggacgg
     3601 cggtggcgac gccggtcaga cggggagtgg cgttgatcgt ctcgccgccg gagccggtgg
     3661 cggtgaccct gagggtgtag gttccgccgt cgggcaggtt ctggccgtcg gtggtgacgc
     3721 cgtcccagtc gagggtgtgt tcgcccccgg cgacggcggc gtctgtgcgg ctccagaccg
     3781 tggtcccctg gctgttcagc acctcgtact tcacgctcga ggcgttgcgg tcgaggtcgt
     3841 aggtccagct ggcggcgccg tcggtcatga cggcctcgtc gctggcggcg gtgaccgtct
     3901 tgccgatcag gtcgaccgat cctgccaggc cgccatcgct catgccgacc agcatcttca
     3961 gcaggtcgtt ggtcatgagc tgctgctcga ccccggtcat ctgcacgatc tgggcggtga
     4021 actcgttgga atcgagcggc gacagcggat cctggttctt cagctgggtg gtcagcaggg
     4081 ccaggaaggt ctgttcgctg tcggcgaggc gattgcgcga gctgctcagc ttttcgttga
     4141 ccgaggcgtt cgcggccgtg gctgtgggat cgatcgtggt ggtcatggcc tgcctcagat
     4201 ccggatatcg acgccggacc gggtccggcg cgtggggttg gcggggaccg tcgcgagcgt
     4261 caggtcggcg tcgtcgaggc tgctctggac ccgctggaag gcgcggccgt tccaggcccg
     4321 gtcttgctgc tgtccggcct cgcgaccgcc ggagcccgcg ccctgaccgg ccgcgtcgaa
     4381 ggtcaggctg ttctccgaca catcgaatcc ggcctgctcg agcgcctgac gcagctcgcc
     4441 ggcccggccg cgcaggtcgg cggcggcctg cggcgagtcg aacgacaggg cggcggtcaa
     4501 cttgccattg cggtcgatct cgatggccac atcgaccttg cccaggccat gcggatccag
     4561 ctgcaggtc
//
LOCUS       DAORNX010000231         4534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14219_length_4534_cov_7.582050, whole genome shotgun sequence.
ACCESSION   DAORNX010000231 DAORNX010000000
VERSION     DAORNX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14219_length_4534_cov_7.582050"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLO65_10565"
     CDS             complement(<1..894)
                     /locus_tag="PLO65_10565"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRD28729.1"
                     /translation="MSDHLRAHDAALERLRLLHPKLIDLSLGRMHRLCAALGDPQRRL
                     PPVVHVAGTNGKGSTVAVLRAIAEAAGLRVHVFTSPHLVRFAERIRLAGTLITDAHLA
                     EVLERVEQANAGEAITFFEITTAAAFQAFSEVEADLCLLEVGLGGSLDATNVLERPAV
                     SVIAPVDHDHREFLGDTIAGIAGEKAGIIKRGAPVVVGRQSEEAFAVIEARALALGAP
                     LLAMGVDFDAWSERARLVVQSQERLLDLPAPALHGPHQIDNAGLAVMAALQLREARID
                     EAAIGRGVASAVWPARFQRLAV"
     gene            complement(1012..1932)
                     /gene="accD"
                     /locus_tag="PLO65_10570"
     CDS             complement(1012..1932)
                     /gene="accD"
                     /locus_tag="PLO65_10570"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRD28730.1"
                     /translation="MAMADPKNDKPTRGNARERQGWLARIAPGVRGAFVKRETPENLW
                     VKCPDTGEMIYRPDLEAAWWVTPAGRHMRIGPELRFKYTFDDGAYEALPTPAVPEDPL
                     HFSDGKPYKDRLVAARKATGESDAMAIGAGRIGGTPAVVLVQDFAFMGGSLGMAAGEA
                     FIAAARAAIAREAPLVVYTAAGGARMQEGTLSLMQMARTTLAIQELKRARLPYIVVLT
                     DPTTGGVTASYAMLGDIHLAEPGALIGFAGPRVIEQTIRETLPPGFQRAEYLVEKGMV
                     DRVVARHELPVVLGSILKTLMMGRNGQKAA"
     gene            complement(1937..2767)
                     /gene="trpA"
                     /locus_tag="PLO65_10575"
     CDS             complement(1937..2767)
                     /gene="trpA"
                     /locus_tag="PLO65_10575"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRD28731.1"
                     /translation="MTNRLTPRFAALATEDRAAFVAYVMAGDPDVATSFEILSRLPAA
                     GADIIELGMPFSDPMAEGPTIQRAAQRSLAAKTRMADVLALVARFRQQDNATPIVLMG
                     YLNPVLFHGYEAFAADAARAGVDGMIIVDCPPEEADPLADALDAVGVSLIRLAAPTTD
                     DVRLPAVVRRTSGFLYYVSVAGVTGVKEAVAASVAEPVRRLRAASGLPVAVGFGIRTP
                     ERAAEVARVADGVVVGSALVDEIEGALLAGSNENVTERVLSKAAQLAKAVRLARVRDA
                     "
     gene            complement(2764..3999)
                     /gene="trpB"
                     /locus_tag="PLO65_10580"
     CDS             complement(2764..3999)
                     /gene="trpB"
                     /locus_tag="PLO65_10580"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRD28732.1"
                     /translation="MNAPSSMTTGKPNDWSAFPDAQGRFGDYGGRFVPETLMPLVLEL
                     EKAYAAADADPDFHGELDGFLKTYVGRPNPLYFAERLTAHIGGAKIYFKREELNHTGA
                     HKINNCMGQILLAMRMGKTRIIAETGAGQHGVASATVCARFGLPCIVYMGAVDVERQK
                     PNVFRMNLLGAEVRPVTSGAGTLKDAMNEAMRDWVTNVEDTYYMIGTAAGPHPYPAMV
                     RDFQSVIGKEIRAQILEAEGRLPDALVACVGGGSNAIGTFHPFLGDEGVKIFGVEASG
                     HGLETDKHAASLTGGRPGVLHGNKTYLLQDDDGQILDAHSISAGLDYPGIGPEHSWLH
                     DVGRARYLTCTDDEALAAFQLCAQLEGIIPALESAHAIAKLPEVAKEVGKDGIVVMTL
                     SGRGDKDVNTVAGALGMSI"
     gene            complement(4012..>4534)
                     /locus_tag="PLO65_10585"
     CDS             complement(4012..>4534)
                     /locus_tag="PLO65_10585"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRD28733.1"
                     /translation="TGDAARLAPPARGKAAVVAVTVDPDDDLLDELMATLKPDLVQLH
                     GKETPARARAVAARTGAGVIKALPVSEPADLDAGRVFETVVEHLMFDARPPADAAMPG
                     GLGSPFDWTILAGRRFARPYLLAGGLDPWNVQQALRLSDAPIVDVSSGVERGPGIKDP
                     GLISAFLEAVRRA"
BASE COUNT          674 a         1553 c         1631 g          676 t
ORIGIN      
        1 gaccgccagg cgctggaagc gggccggcca gaccgccgag gccacgcccc gcccgatcgc
       61 cgcctcgtcg atgcgcgcct cgcgcagctg cagggcggcc atgaccgcaa ggccggcgtt
      121 gtcgatctga tgcggtccgt ggagggccgg ggcgggcagg tccagcaacc gctcctggct
      181 ctgcaccacc agccgcgcgc gttcggacca ggcgtcgaag tccacgccca tggccagcag
      241 gggcgcgccg agcgccaggg cgcgggcctc gatcaccgcg aacgcctctt ccgactgccg
      301 gccgaccacc accggcgcgc cacgcttgat gatgccggcc ttctcgccgg cgatgccggc
      361 gatggtgtcg ccgaggaact cgcggtgatc atggtcgacc ggggcgatca cgctgaccgc
      421 cggccgttcg agcacgttgg tcgcgtcgag ggatccgccg agacccactt ccagcaggca
      481 gagatcggcc tcgacctcgg agaaggcctg gaaggcggcg gccgtggtga tctcgaagaa
      541 ggtgatcgcc tcgccggcgt tggcctgttc cacccgttcc agcacctcgg ccagatgggc
      601 gtcggtgatc agggtccccg ccagccggat gcgctcggcg aaccgcacca gatggggcga
      661 ggtgaagacg tgcacgcgca ggccggccgc ctcggcgatg gcgcgcagga cggcgaccgt
      721 cgatcccttg ccgttggtgc cggcgacatg aaccaccggc ggcagcctgc gctggggatc
      781 gccgagcgcc gcgcacaggc ggtgcatccg ccccagcgac aggtcgatca gcttgggatg
      841 cagcaggcga agccgctcaa gcgcggcgtc atgggctcga aggtggtctg acacgaaatc
      901 cgctcacccc ggcgaaggca gggacccagg tttgtttgtg ttcagttctg cccgcaccat
      961 ggcttcgcaa aagcctgggt tccggccttc gccggaatga gcggaaattt gctacgccgc
     1021 cttctggccg ttccgcccca tcatcagggt cttgaggatc gagcccagca ccaccggtag
     1081 ctcgtggcgg gcgaccacgc gatcgaccat gcccttttcc accaggtatt cggcgcgctg
     1141 gaagccgggg ggcagggtct cgcggatggt ctgttcgatc acgcgcgggc cggcgaagcc
     1201 gatcagggcg cccggctcgg ccaggtggat gtcgccgagc atggcgtagc tggcggtgac
     1261 gccgcccgtc gtcggatcgg tcagcacgac gatgtagggc agccgggcgc gcttgagctc
     1321 ctggatcgcc agggtcgtgc gggccatctg catcagggac agggtgccct cctgcatccg
     1381 ggccccgccc gctgcggtgt agacgaccag cggggcctcg cgcgcgatcg ccgcgcgggc
     1441 ggcggcgatg aaggcctcgc ccgcggccat gcccagcgag ccgcccatga aggcgaagtc
     1501 ctggaccagc accacggcgg gggtcccgcc aatgcggccg gcgccgatgg ccatggcgtc
     1561 ggattcgccg gtcgccttgc gggcggccac gaggcgatcc ttgtagggct tgccgtcgga
     1621 gaaatggagc gggtcctcgg gcacggccgg cgtcggcagg gcctcgtagg cgccgtcgtc
     1681 gaacgtgtac ttgaagcgca gttccgggcc gatccgcata tgccggccgg cgggggtcac
     1741 ccaccaggcg gcttccaggt cggggcggta gatcatttcc ccggtgtcgg ggcatttgac
     1801 ccagaggttc tcgggcgtct cgcgcttgac gaaggcgccg cggacgccag gggcgatccg
     1861 ggcgagccag ccctggcgct cgcgcgcgtt gcctcgggtc ggtttgtcgt tcttggggtc
     1921 agccatcgcc atcgcttcaa gcatccctga ctcgcgccaa gcgtacagcc ttagccagct
     1981 gcgccgcctt ggaaagaact ctttcagtca cgttttcgtt cgacccggcc agcagggcgc
     2041 cttcgatttc atcgaccagg gccgatccga cgacgacgcc gtcagccacc cgggcgactt
     2101 cggccgcccg ctcaggggtg cggatgccga acccgaccgc cacgggcagt cccgacgccg
     2161 cgcgcagccg gcgcacgggc tcggcgacgg aggcggcgac ggcctccttc acgccggtca
     2221 ccccggcgac ggatacataa tagagaaagc ccgaagtgcg ccggaccacg gccggcaggc
     2281 ggacgtcgtc ggtggtcggg gcggccagcc ggatcagcga caccccgacc gcgtcgaggg
     2341 cgtcggccag cggatcggcc tcctcaggcg ggcagtcgac gatgatcatg ccgtccaccc
     2401 cggcccgggc cgcatcggcg gcaaaggcct cgtagccgtg gaacagcacg gggttgaggt
     2461 agcccatcag gacgatcggg gtggcgttgt cctgctggcg gaagcgggcg accagggcca
     2521 gcacgtcggc catcctggtc ttcgccgcca gcgaccgctg cgcagcgcgc tggatcgtcg
     2581 gcccctcggc catcgggtcg gagaagggca tgcccagctc gatgatgtcg gcgccggcgg
     2641 ccggcaacct cgacaggatc tcgaacgacg tggccacgtc gggatcgccg gccatgacat
     2701 aggcgacgaa ggcggcgcgg tcctccgtgg cgagagccgc gaagcgcggg gtcagtctgt
     2761 tggtcaaatc gacattccca gcgcgccggc gacggtgttg acgtccttgt cgccccggcc
     2821 cgacagggtc atgaccacga tcccgtcctt gccgacctct ttcgccacct cgggcagctt
     2881 ggcgatggcg tgggcgctct ccagggcggg gatgatcccc tccagctggg cgcagagctg
     2941 gaacgccgcc agggcctcgt catcggtgca ggtcaggtag cgcgcccggc cgacgtcgtg
     3001 cagccagctg tgctccgggc cgatgcccgg atagtcgagg ccggcgctga tcgagtgggc
     3061 gtcgaggatc tggccgtcgt cgtcctgcaa caggtaggtc ttgttgccat gcaggacgcc
     3121 ggggcggccg ccggtcaggg atgcggcgtg cttgtcggtc tccaggccgt gacccgaggc
     3181 ctcgacgccg aaaatcttca ccccctcgtc gccgaggaag ggatggaagg tgccgatggc
     3241 gttggacccg ccgccgacgc aggcgaccag ggcgtcgggc aggcggccct cggcctcgag
     3301 gatctgcgcg cggatttcct tgccgatcac cgactggaag tcgcggacca tggccggata
     3361 ggggtgcggg ccggcggccg tgccgatcat gtagtaggtg tcctcgacgt tggtcaccca
     3421 gtcgcgcatg gcctcgttca tggcgtcctt gagtgtgccg gcgcccgagg tgaccggccg
     3481 cacctcggcg cccagcaggt tcatgcggaa gacattgggc ttctgccgct ctacgtcgac
     3541 cgcgcccatg tagacgatgc agggcaggcc gaaacgggcg cagacggtgg cgctggccac
     3601 gccgtgctgg cccgcgccgg tctcggcgat gatgcgggtc ttgcccatgc gcatggccag
     3661 caggatctgg cccatgcagt tgttgatctt gtgcgcgccg gtgtgattca gctcctcgcg
     3721 cttgaagtag atcttcgccc cgccgatgtg ggcggtcagc cgttcggcga aatacagcgg
     3781 gttcggccgg ccgacatagg tcttcaggaa gccatcgagc tcgccatgga agtccgggtc
     3841 ggcgtctgcc gcggcgtagg ccttctccag ctcgagcacg agcggcatca gggtttcggg
     3901 cacaaaacgg ccgccgtagt cgccgaagcg cccctgggcg tccggaaagg cggaccagtc
     3961 gttgggcttg ccagtcgtca tcgaagaagg ggcgttcacg gcgtcagaca ctcaggcgcg
     4021 gcgcaccgcc tccagaaagg ccgaaatcag gccggggtcc tttatccccg ggccgcgctc
     4081 cacgccagag gaaacgtcca cgatcggggc gtccgacagc cgcagggcct gctggacatt
     4141 ccacggatcg aggccgcccg cgaggaggta gggccgggcg aaccggcgtc cagccagaat
     4201 cgtccagtcg aagggcgacc ccaggccgcc gggcatggca gcgtcggccg gcggcctggc
     4261 gtcgaacatc agatgctcga ccaccgtctc gaacacacgg ccggcgtcga ggtcggccgg
     4321 ttccgagacc ggcagggcct tgatcacgcc ggcccccgtg cgggcggcca ccgcccgggc
     4381 gcgcgccggc gtctccttgc cgtgcagttg aaccagatca ggcttcagcg tcgccatcag
     4441 ttcgtcgagc aggtcatcat cgggatcgac ggtcaccgcc acgaccgccg ccttgccgcg
     4501 cgccggcggg gccagccggg cggcgtcgcc ggtc
//
LOCUS       DAORNX010000232         4530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14235_length_4530_cov_8.052291, whole genome shotgun sequence.
ACCESSION   DAORNX010000232 DAORNX010000000
VERSION     DAORNX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_4530_cov_8.052291"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLO65_10590"
     CDS             <1..264
                     /locus_tag="PLO65_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28734.1"
                     /translation="GVLESQLAADDFAFVAGALPAAPTAPASALSSVDAMPALDSRAG
                     VDLWSAATIQDAQGDAEPGLGLGGPGFERLHLFAAPSDYDWVW"
     gene            392..>4530
                     /locus_tag="PLO65_10595"
     CDS             392..>4530
                     /locus_tag="PLO65_10595"
                     /inference="COORDINATES: protein motif:HMM:NF020137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD28735.1"
                     /translation="MYRLQNELMSSVWTWNGLDDRAFGAGERGPGQAGAVSERFVAVM
                     TSSGADTPVGPAGDAYVRIPLPAGDPIAQFKAAAPGVTDASAAASPGVIPQFDQMRPL
                     QDLLHPELNNANGAGRTELITQTYNAGLGRYEFTGVQETDAVLIGSRWTPTTLTYSFP
                     TSGDFYADQGYGAGAEPAELVVFNAAQQAATRYAFGLISAYTNLTFVEVTESATTHAD
                     FRLSQTGLDSVGSAYANFPSSSAQAGDVWFGMTGQPFYDTPAPGNWGQATIMHELGHA
                     LGLKHGHDNYTSYDLAGGGYLDAPPGGGARYGTVALPSEHDGQSWSLMTYRSSPGAPI
                     DFQGDGFNQPQTYMQDEIAALQHLYGANFNTQSGNTVYTWNPTTGEQSINGVGQGAPT
                     GNIILSTLWDGNGVDTYDLSNYSTNLSINLEPGEFSTFSSSQLANHRAYSGGNAPAAG
                     NVANARLFQGDTRSLIENANGGTGDDTLIGNQAANTLTGNNGADTLIGNVGNDTLIGG
                     AGNDALYGDGKASAPSGVVIGDGVVTKASGLGNVSTATALDITSEFSLAANGDILNAT
                     TVPHVTISGTGDGNKDYYKIVLTAGAVITLDVDHSVGIDSFIRLLDGNGNVLAFDDDS
                     ATTEGASGSASSLDSFLSYTVATGGTYYIEIGNYATSTTNGPIDAGDTYDVQVSVADL
                     PSVGSGSGDDTLIGGAGGDSLNGGDGIDTISYVGSALAVYVRLFNNTAFGGDAQGDVI
                     SGFENITGSSNNDDLRGSTGANVLDGGAGNDLLIGFAGADTLIGGDGIDTVSYVGSSS
                     AVFVRLFNNTAFGGDAQGDVISGFENITGSSNNDDLRGSTGANVLDGGAGNDLLIGFA
                     GADTLTGGDGIDTLSYVGSATAVTVNLLTNIVSGGDAQGDVISGFENLTGSSNNDVLT
                     GNAGANVLDGGLGGDTLIGGDGIDTISYVGSSAGVSVRLFNNTAFGGDAQGDVISGFE
                     NITGSSNNDDLRGSTGANVLDGGAGNDLLIGFAGADTLIGGDGLDTISYAGSSQAVFV
                     RLFNNTAFGGDAQGDVISGFENITGSSNNDDLRGSTGANVLDGGAGDDLLIGFAGADT
                     LTGGDGIDTVSYVGSSAAVFVRLFNNLAFGGDAQGDVISGFENIIGSSNNDDLRGGND
                     INVLDGGAGNDILMGFAGADTLIGGAGGDTLTGGDGIDTVSYVGSSAGVFVRLFNNLT
                     FGGDAQGDVISGFENIIGSSNNDDLRGGNDINVLDGGAGNDILMGFAGADTLIGGAGG
                     DTLTGGDGLDTISYVGSAAAVTVNLLTNTVSGGDAAGDVISEIENITGSSNNDILTGN
                     AGANVLNGGAGGDTLTGGSGLDTISYVGSSAGVFVRLFNNLAFGGDAQGDVISGFENI
                     IG"
BASE COUNT          800 a         1523 c         1492 g          715 t
ORIGIN      
        1 ggggtgctcg aaagccagct ggccgccgac gacttcgctt tcgtcgccgg cgccctgccg
       61 gccgcgccga cggcgccggc ctcggcgctg tcgtccgtcg acgccatgcc ggcgcttgac
      121 tccagggccg gcgtcgacct gtggagcgcc gcgacaatcc aggacgcgca aggcgacgcc
      181 gagcctggtc tgggcctcgg cggcccgggc tttgaacggc tgcacctgtt cgccgcgccc
      241 tcggactacg actgggtctg gtagtcgacc tccgcttcaa gcggaagtga tgctcgtgtc
      301 cggaacaaag gccttggccc ccgtcacgct ccgcgcctaa catcgacctt tgttatccca
      361 aaattccttc cacgaccgca ggggcctggc catgtaccgt ctgcaaaacg aactgatgtc
      421 gtcagtctgg acctggaacg gcctcgatga ccgcgccttc ggggccggtg agcgcggccc
      481 cggccaggcc ggggccgtga gcgaacgctt cgtcgccgtc atgaccagtt cgggcgccga
      541 tacgcccgtc ggccccgccg gcgacgccta tgtccggatt ccccttcccg cgggggaccc
      601 catcgcccag ttcaaggccg ctgctcccgg cgtgaccgac gcctcggccg cggcctcgcc
      661 cggtgtcatt ccgcaattcg accagatgcg tccgctgcag gacctgctgc atccggagct
      721 caacaacgcc aacggcgccg gccgcaccga actgatcacc cagacctaca acgccggcct
      781 ggggcgctac gagttcaccg gcgtgcagga gaccgacgcg gtgctgatcg gctcgcgctg
      841 gacccccacg accctgacct acagcttccc gaccagcggc gacttctatg ccgaccaggg
      901 ttacggcgcc ggcgccgagc cagccgaact ggtggtgttc aacgccgccc agcaggccgc
      961 gacccggtat gcgttcggcc tgatttcagc ctacaccaac ctgaccttcg tcgaggtcac
     1021 cgagtcggcc acgacccatg ccgatttccg cctgagccag acgggcctgg acagcgtcgg
     1081 ttccgcctac gccaacttcc cgtccagctc cgcccaggcc ggcgatgtct ggttcggcat
     1141 gaccggccag ccgttctacg acacgcccgc cccgggcaac tggggccagg cgaccatcat
     1201 gcacgagctc ggccacgccc tgggcctgaa gcacggacat gacaactaca cgtcctatga
     1261 cctggccggc ggcggttatc tcgacgcgcc tccgggcggc ggcgcccgct acggcaccgt
     1321 ggcgctcccg tcggagcacg atggccagtc ctggtcgctg atgacctacc gttcgtcgcc
     1381 cggggccccg atcgacttcc agggcgacgg gttcaaccag ccgcagacct acatgcagga
     1441 cgagatcgcg gcgctgcaac acctctacgg ggccaacttc aacacccaga gcggcaacac
     1501 ggtctacacc tggaacccga ccacgggcga gcagtcgatc aatggcgtcg gccagggcgc
     1561 gccgacgggc aacatcattc tctcgaccct gtgggacggc aacggcgtcg atacctacga
     1621 cctgtcgaac tacagcacca acctgagcat caatctcgag ccgggcgagt tctcgacctt
     1681 ctcgtcgagc cagctggcca accaccgggc ctacagcggc ggcaacgcgc ccgcggccgg
     1741 caacgtcgcc aatgcccgcc tgttccaggg cgacacccgc tcgctgatcg agaacgcgaa
     1801 cggcggaacc ggcgacgaca ccctgatcgg caaccaggcg gccaacaccc ttacgggcaa
     1861 caacggcgcc gacaccctga tcggcaacgt cggcaatgac accctgatcg gcggagccgg
     1921 caacgacgcc ctctacggcg acggcaaggc ctccgccccg tccggggtgg tcatcggcga
     1981 cggcgttgtg accaaggcct ctggcctcgg caatgtcagc acggcgacgg cgctcgacat
     2041 caccagcgag ttcagcctgg cggccaacgg cgacatcctc aacgcgacca ccgtgcccca
     2101 tgtcaccatc agcggaaccg gcgacggcaa caaggactac tacaagatcg tcctgaccgc
     2161 gggcgcggtg atcaccctgg acgtcgacca ctcggtcggg atcgacagct tcatccgcct
     2221 gctcgatggc aacggcaacg ttctggcgtt cgatgacgac agcgcgacca ccgagggggc
     2281 cagcggatcg gcctccagcc tggattcatt cctgagctac acggtcgcca cgggcgggac
     2341 ctactatatc gagatcggca actacgccac ctcgacgacc aatggcccga tcgacgccgg
     2401 cgacacctat gacgtccagg tttcggtcgc cgacctgccc tcggtcggca gcggatcggg
     2461 cgatgacacc ctgatcggcg gagcgggcgg cgactcgctc aatggcggcg acgggatcga
     2521 caccatcagc tacgtcggct cggccctggc ggtctatgtg cggctgttca acaacaccgc
     2581 cttcggcggc gacgcccagg gcgatgtgat cagcggcttc gaaaacatca ccggctcgtc
     2641 gaacaatgac gacctgcggg gctcgaccgg ggccaatgtt ctggacggcg gggctggaaa
     2701 cgatttgctg atcggctttg ccggggccga cacgctgatc ggcggtgatg gcatcgacac
     2761 ggtgagctac gtcgggtcgt cgtcggcggt gttcgtgcgg ctgttcaaca acaccgcctt
     2821 tggcggtgac gcccagggcg atgtgatcag cggcttcgaa aacatcaccg gctcgtcgaa
     2881 caatgacgac ctgcggggct cgaccggggc caatgttctg gacggcgggg ctggaaacga
     2941 tttgctgatc ggctttgccg gggccgacac cctgaccggc ggcgacggca tcgacacgct
     3001 gagctacgtc gggtcggcaa cggcggtcac agtcaacctg ctaaccaaca tagtgtcggg
     3061 cggtgacgcc cagggcgatg tgatcagcgg cttcgagaac ctcaccggct cgtcgaacaa
     3121 cgacgtcctg accggcaacg ccggggccaa cgtgctcgat ggcggtcttg gcggcgacac
     3181 actgatcggc ggcgacggga tcgacaccat cagctacgtc gggtcgtcgg cgggggtgtc
     3241 tgtacggctg ttcaacaaca ccgcttttgg cggggacgcc cagggcgatg tgatcagcgg
     3301 cttcgagaac atcaccggct cgtcgaacaa tgacgacctg cggggctcga cgggcgccaa
     3361 tgttctggac ggcggggctg gaaacgattt gctgatcggc tttgccggag ccgacaccct
     3421 gatcggcggt gatggcctgg ataccatcag ctacgccggc tcgtcacagg cggtgttcgt
     3481 acggctgttc aacaacaccg cctttggcgg tgacgcccag ggcgatgtga tcagcggctt
     3541 cgaaaacatc accggctcgt cgaacaatga cgacctgcgg ggctcgaccg gggccaatgt
     3601 tctggacggc ggggctggag acgatttgct gatcggcttt gccggagccg acaccctgac
     3661 cgggggcgac gggatcgaca cggtgagcta cgtcgggtcg tcggcggcgg tgtttgtgcg
     3721 gctgttcaac aacctggcct ttggcggaga cgcccagggc gatgtgatca gcggcttcga
     3781 gaacatcatc ggctcgtcga acaacgacga cctgcggggc ggcaacgaca tcaatgtgct
     3841 ggatggcggg gcgggcaacg acattctgat gggcttcgcc ggagccgata cgctgatcgg
     3901 cggggcgggc ggcgacaccc tgaccggggg cgacgggatc gacacggtga gctacgtcgg
     3961 gtcgtcggcg ggggtgtttg tgcggctgtt caacaacctt acctttggcg gtgacgccca
     4021 gggcgatgtg atcagcggct tcgagaacat catcggctcg tcgaacaacg acgacctgcg
     4081 gggcggcaac gacatcaatg tgctggatgg cggggcgggc aacgacattc tgatgggctt
     4141 cgccggagcc gatacgctga tcggcggggc gggcggcgac accctgaccg gcggcgatgg
     4201 cctggacacc atcagctacg tcggatcggc ggcggcggtc acggtcaacc tgctgaccaa
     4261 cacggtgtca ggcggggatg cggcggggga tgtgatctcg gagatcgaga acatcaccgg
     4321 ctcgtcgaac aacgacatcc tgaccggcaa tgccggagcc aatgtgctca atggcggggc
     4381 tggcggcgac accctgaccg gcggcagcgg cctggacacc atcagctacg tcgggtcgtc
     4441 ggcgggggtg tttgtgcggc tgttcaacaa cctggccttt ggcggtgacg cccagggcga
     4501 tgtgatcagc ggcttcgaga acatcatcgg
//
LOCUS       DAORNX010000233         4517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14278_length_4517_cov_7.276782, whole genome shotgun sequence.
ACCESSION   DAORNX010000233 DAORNX010000000
VERSION     DAORNX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_4517_cov_7.276782"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..>444)
                     /locus_tag="PLO65_10600"
     CDS             complement(97..>444)
                     /locus_tag="PLO65_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD28736.1"
                     /translation="SYFLSKLVGSARARELYFTADVITGAEAARLGVVNRAVPAADLA
                     REAAALARRLADLPTVAVGYMKKNLAIAEHGTLSETMDAEAIHMVRTMMTEDHKAASL
                     AFVEKRPPVFRGR"
     assembly_gap    445..680
                     /estimated_length=236
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<681..900)
                     /locus_tag="PLO65_10605"
     CDS             complement(<681..900)
                     /locus_tag="PLO65_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD28737.1"
                     /translation="MTDQLIETFEDGIATLTMNRPQARNALTGEMLAALSAAAPRLAL
                     DPNVRAVVITGAGGAFCAGGDVKGFVADK"
     gene            1095..2354
                     /locus_tag="PLO65_10610"
     CDS             1095..2354
                     /locus_tag="PLO65_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28738.1"
                     /translation="MNKIVDIRPDAEAELNNLAMSGSAKPLMEAVKRHIAENVEPITE
                     EFFRLGETRKDRWSWAPGQLELLETAKDKAKASGLWNFFLPESEIGRGLTNLDYAYIA
                     VELGKSPLASESLNCSAPDTGNMEVLERVGTPEQKERWLKPLLNGEIRSCYAMTEPGV
                     ASSDAKNIATRAELVGDQWVINGEKYYISGAGDPRCKIMICMVKTSPHAPAQFQQSQI
                     LIPMDTPGVEIIGPMHVFGHDHAPRGHMHLKLNNVRVPKENILLGEGRGFEISQVRLG
                     PGRIHHCMRSIGKAERALDLMVKRATTRVAFGKPLIQLGKNLELVSRARIEIEAMRLM
                     VLKAAKAMDVLGNREARVWVSMVKAMVPEKVCEIIDQAIQIHGATGLSEWTPLAELYT
                     DVRHLRFADGPDEVHHMVVGRAELSQH"
     gene            complement(2395..3267)
                     /locus_tag="PLO65_10615"
     CDS             complement(2395..3267)
                     /locus_tag="PLO65_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28739.1"
                     /translation="MRDVSGKTAFITGGASGIGLAMAEAFGSAGMSVMLADIEAAALA
                     EAVRGLEERQIRAAGVLCDVAHRAALQAAAEKTIETFGKVHVVCNNAGVGAGGPIGEV
                     AAADWDWVMEVNLMGVVYGMETFLPHLRAHGEGGYVVNTASMAGLISPPGMEPYCASK
                     YAVVALSEGWAAQLAPENIGVGVLCPGFVRTNIDRSRRNRPDRFGGPEAPENLAASEF
                     VQGGIDPSRVGLRVLEAIRNDERYIFTHPGMRAAVQERFARILTGFDQADASQALRGL
                     EEPAFPVEGMSPPQ"
     gene            complement(3279..>4517)
                     /locus_tag="PLO65_10620"
     CDS             complement(3279..>4517)
                     /locus_tag="PLO65_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HRD28740.1"
                     /translation="VVRRAIEVVDGAAGLPAAQSPLMLPFDTLPASVDAATRAALRAE
                     GVLIVETRVKPAQRRLVTFLRDSYLPRARPALGVSTLPGGAAYYAFVVRRETTTDMTP
                     AAIHQLGLAEVARIRAAMQAVMDEAGFVGDPRAFSDHLRADPASYAPSAEVYAEKAGE
                     IAKRIDYLLPRYFGVLPRLTYGVRAKPAALESTSDGYLAGSPEAGQAGMVVYSATAAP
                     HEPLYNLPAWLLHEGVPGHHLQIALAQENTTLPEYRRNDDITAYVEGWALYAERLGEE
                     MGVYRTPQERFGRLSMEMWRACRLVMDTGLHAMGWTRDQAAACLKDNTAMTEAAIYGE
                     VDRYIGWPGQALGYKIGELRITAMRRQAETALGARFDLRAFHDVLLGAGALPMDLLQA
                     RIDAWVAGRPPASPAIANAS"
BASE COUNT          666 a         1498 c         1491 g          626 t
ORIGIN      
        1 tggcccggcc gatgccgctg gcggcgccgg tgatgacgat gatgcggtcc ttgagatcca
       61 tggcgcggcc cctccccggg ctgattgcag gtctggttat cggccgcgga acacgggcgg
      121 gcgcttctcg acgaaggcca gggacgcggc cttgtggtcc tcggtcatca tggtgcggac
      181 catgtggatc gcctcggcgt ccatggtctc cgacagcgtg ccatgctcgg cgatggcgag
      241 gttcttcttc atgtagccga cggccaccgt cggcagatcg gccaggcgac gggcgagagc
      301 cgcggcctcg cgggccaggt cggcggccgg cacggcgcgg ttgaccacgc ccagccgcgc
      361 cgcctcggcg ccggtgatca catcggcggt gaaatagagc tcccgcgccc tggcggaacc
      421 gaccagcttg ctgaggaagt agctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn ccttgtccgc cacgaagccc ttcacgtcgc cgccagcgca
      721 gaaggcgccg cccgcgccgg tgatgaccac ggcgcggaca ttcgggtcga gcgccagccg
      781 cggcgcggcc gccgaaagcg cggccagcat ctcgccggtc agggcgttgc gcgcctgcgg
      841 ccggttcatt gtcagggtgg cgatgccgtc ctcgaaggtc tcgatcagct ggtcggtcat
      901 ggtcgtcgtc cttgttcggc ggcgcgggcg tcgggatcga tcgccagcct agatcagggt
      961 ccggaggcgg gccaccgcga gatttgcgtt gaccccgcgg cgcccccctt ccagggtgcc
     1021 ggtcaaacag ttgtttgacg cagagtcggg cgcgccgcaa tcggcgtgtt gcccgccgct
     1081 cccgggagac cgccatgaac aagatcgtcg acatccgtcc cgacgccgag gccgagctga
     1141 acaacctggc catgtcgggc tcggccaagc ccctgatgga ggccgtcaag cgccacatcg
     1201 ccgagaacgt cgagccgatc accgaggagt tcttccggct gggcgagacc cgcaaggatc
     1261 gctggagctg ggcgccggga cagctggagc tgctcgaaac cgccaaggac aaggccaagg
     1321 cctccggcct gtggaacttc ttcctgcccg agagcgagat cggccgcggc ctgaccaacc
     1381 tcgactacgc ctatatcgcc gtagagctgg gcaagagccc gctggcgtcg gagagcctca
     1441 actgctcggc gcccgacacc ggcaacatgg aagtgctcga gcgggtcggc acgcccgagc
     1501 agaaggagcg ctggctcaag ccgctgctga acggcgagat ccgctcctgc tacgccatga
     1561 ccgagccggg cgtggcctcg tccgacgcca agaacatcgc cacccgggcc gagctggtcg
     1621 gcgaccaatg ggtcatcaac ggcgagaaat actacatctc cggcgccggc gatccgcgct
     1681 gcaagatcat gatctgcatg gtcaagacca gtccgcacgc gcctgcgcag ttccagcagt
     1741 cgcagattct gatcccgatg gacacgccgg gggtcgagat catcggaccg atgcatgtgt
     1801 tcggccatga ccacgcgccg cgaggccata tgcacctgaa gctgaacaac gtccgggtgc
     1861 cgaaggagaa catcctgctc ggcgagggtc gcggcttcga gatcagccag gttcggttgg
     1921 gtccggggcg gatccaccac tgcatgcgct cgatcggcaa ggccgaacgg gccctcgatc
     1981 tgatggtcaa gcgggccacc acccgtgtcg ccttcggcaa gccgttgatc cagctgggca
     2041 agaacctgga gctggtcagc cgcgcccgca tcgagatcga ggccatgcgg ctgatggtgc
     2101 tgaaggcggc caaggccatg gacgtgctgg gcaaccgcga ggcccgcgtc tgggtcagca
     2161 tggtcaaggc catggtgcct gagaaggtct gcgagatcat cgaccaggcg atccagatcc
     2221 acggcgccac cggcctgtcg gaatggacgc cgctggccga gctctacacc gacgtgcgcc
     2281 acctgcgctt cgccgatggt ccggacgagg tgcaccacat ggtggtcggc cgggccgagc
     2341 tgagccagca ctgatcagcc gggcccgcgc ttccctgacc cgaagggcgg gccctcactg
     2401 cggcggcgac atgccctcga ccggaaacgc cggctcctcc aggccccgca gcgcctggct
     2461 ggcgtcggcc tggtcgaagc cggtcaggat gcgggcgaaa cgctcctgca ccgcggcgcg
     2521 catgcccgga tgggtgaaga tgtagcgctc gtcgttccgg atcgcctcca gcacccggag
     2581 gccgacccgc gacgggtcaa tgccgccctg gacgaactcc gacgccgcca ggttttccgg
     2641 cgcctccggg ccgccaaaac ggtcgggccg gttgcgtcgg gaacgatcga tgttggtcct
     2701 gacaaagccc ggacagagga cgcccacgcc gatgttctcc ggcgccagct gggcggccca
     2761 gccctccgac agggcgacga ccgcgtactt gctggcgcaa tagggctcca tgccgggagg
     2821 cgagatcaga ccggccatcg aggcggtatt gaccacgtaa ccaccctcgc cgtgggcccg
     2881 caggtgcggc aggaaggtct ccatgccgta gaccacgccc atcagattga cctccatcac
     2941 ccagtcccag tccgccgccg cgacctcgcc gatcggcccg ccggccccga cgccggcgtt
     3001 gttgcagacc acgtggacct tgccgaaggt ctcgatcgtc ttctcggcgg ctgcctgcag
     3061 ggcggcgcgg tgggcgacat cacacagcac gcccgccgcg cggatctgtc gttcctccag
     3121 gcctcgcacc gcctcggcca gcgccgccgc ctcgatgtcg gccagcatca ccgacatgcc
     3181 cgcagacccg aaggcctcgg ccatggccag gccgatgccg ctggccccgc cggtgatgaa
     3241 ggccgtcttg ccgctgacgt cacgcatggg atttcccttc aggatgcatt ggctatcgcc
     3301 ggcgacgccg gaggccgccc cgccacccag gcgtcgatgc gcgcctgcag caggtccatc
     3361 ggcagggccc ccgcgccaag caggacgtca tggaaggcgc gcaggtcgaa gcgggcgccc
     3421 agggccgttt ccgcctgccg gcgcatggcc gtgatgcgaa gctcgccgat cttgtagccc
     3481 agcgcctggc ccggccagcc gatgtagcgg tcgacctcgc catagatggc cgcctcggtc
     3541 atggccgtgt tgtccttcag acaggcggcc gcctggtcgc gcgtccagcc catggcgtgg
     3601 aggcccgtgt ccatcaccag gcggcaggcg cgccacatct ccatggacag gcggccgaac
     3661 cgttcctgcg gcgtgcggta gacgcccatc tcctcgccca gccgttcggc gtagagcgcc
     3721 cagccctcga cataggccgt gatgtcgtcg ttgcggcgat actcgggcag ggtcgtgttt
     3781 tcctgggcca gggcgatctg caggtggtgg cccggcacgc cctcgtgcag cagccaggcc
     3841 ggcaggttgt agagcggctc gtgcggcgcg gccgtggcgc tgtagaccac catgccggcc
     3901 tgaccggctt ccgggctgcc cgccagatag ccgtcggagg tgctttccag cgccgccggc
     3961 ttggcccgca cgccgtaggt caggcgcggc aggacgccga aatagcgcgg caggaggtag
     4021 tcgatgcgct tggcgatctc gccggccttc tcggcataga cctcggccga cggggcgtag
     4081 gaggccggat cggcccgcag atggtcggag aaggcgcgtg ggtcgccgac gaagcccgct
     4141 tcgtccatca ccgcctgcat cgccgcgcgg atgcgggcga cctcggcgag accgagttgg
     4201 tggatcgcgg ccggcgtcat gtcggtggtc gtctcgcgcc ggacgacgaa ggcgtagtag
     4261 gccgcgccgc ccggcagagt cgacaccccg agcgcggggc gggcccgtgg caggtagctg
     4321 tcccgcagga aggtcacgag tcggcgctgg gccggcttga cccgggtctc gacgatcagc
     4381 acgccctcgg cgcgcagggc ggcgcgcgtc gcggcgtcga cggacgccgg cagcgtgtcg
     4441 aacggcagca tcagcgggct ctgcgccgcg ggcagacccg ccgccccgtc gaccacctcg
     4501 atcgcccggc ggacgac
//
LOCUS       DAORNX010000234         4512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14300_length_4512_cov_7.220103, whole genome shotgun sequence.
ACCESSION   DAORNX010000234 DAORNX010000000
VERSION     DAORNX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14300_length_4512_cov_7.220103"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>232
                     /locus_tag="PLO65_10625"
     CDS             <1..>232
                     /locus_tag="PLO65_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRD28741.1"
                     /translation="ALARLLDGVLLDLAPVALDAGFLGPRAADWLGAAAKGGPEAPLA
                     FHMDPLSAFAEAGVSPGPVESHIVSAATVGARL"
     assembly_gap    233..355
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            384..>664
                     /locus_tag="PLO65_10630"
     CDS             384..>664
                     /locus_tag="PLO65_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRD28742.1"
                     /translation="MEDAFGRIALGVSIDGEYFTGVAKVRAVRALWARITGACGVAIP
                     ARIEARSSRRMLTALDPWTNLIRLTAAGFAAAVGGANTIVLGAFTDALGL"
     assembly_gap    665..735
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <736..1084
                     /locus_tag="PLO65_10635"
     CDS             <736..1084
                     /locus_tag="PLO65_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRD28743.1"
                     /translation="AGGAWYLDQLTDDLARAAWTRFQAIEAGGGIVAALSSGVIAADV
                     AASRQATETAVREGGVKLLGVTVFRNADARPVEVEAAEPVAVSGPEIRLPGPDSLCPP
                     LAPARLSAAFEEA"
     gene            1088..3235
                     /gene="scpA"
                     /locus_tag="PLO65_10640"
     CDS             1088..3235
                     /gene="scpA"
                     /locus_tag="PLO65_10640"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRD28744.1"
                     /translation="MASFPNFAELAFEEAATSAPAAEGHVWETPEGIPVSAAYDADDV
                     AGLDFTGGYPGIEPFVRGPYPTMYATNPWTVRQYAGFSTAEDSNAFYRRNLAAGQMGL
                     SVAFDLATHRGYDSDHERVKGDVGMAGVAIDSILDMRTLFDGIPLDKMSVSMTMNGAV
                     LPILALYIVAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFSYTS
                     SKMPKFNSISISGYHMQEAGATADLELAYTLADGIEYVRAGIAAGMTVDQFAPRLSFF
                     WAIGMNYFMEVAKMRAARLLWARLMKREFDPKDSRSLSLRTHSQTSGWSLAAQDVFNN
                     VARTCVEAMAAVNGQTQSLHTNSLDEALALPTDFSARISRNTQLFLQMESGTTRVADP
                     WGGSYYVERLTHDLAARALEHIDEVEKLGGMAKAIEQGLPKLRIEEAAARTQARIDSG
                     RQSVVGVNRYKPEVADDIPVLKVDNTAVRNAQLEKLKRLKSERDEAETLAALAALEAG
                     ARADGNLLELAVNAARAKATVGEISYALEKVFGRHRADIKSIQGVYVSEAGSNAAVAR
                     AKAMVEAFEQADGRRPRILIAKMGQDGHDRGQKVIATAFADLGFDVDIGPLFQTPAEA
                     ARQAVENDVHVVGASSLAAGHLTLAPELIAELGKAGRPDIMTVIGGVIPPQDFQALTD
                     AGVVAIFPPGTVIAEAAIDLLEKLNRQLGYDQA"
     gene            3249..4205
                     /locus_tag="PLO65_10645"
     CDS             3249..4205
                     /locus_tag="PLO65_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28745.1"
                     /translation="MKFRPFTMPDEIGSEPDPDLEQYFTLYRDELFDGFVGHGLVSIR
                     QDWPPDLIGKIAAAVVSNNLGIGLDHAYKRYCPPENYRRNDDPEVPKLDAQFERDFDN
                     LCFALNESYAFEKADDAPEQDFFSAFGILRARGMLDAAHALAQQGYLSETLILVRGSL
                     EILAWAAEASVFQEDYRPLELTTQFAITKSKATIPGLAQIYGTLSKFAHWHPDTHLAF
                     LLGRSGRTAVRQRSVEHKAAALLAVASGQLTVAITFLANMQRWYPTHDMSDVRAAASQ
                     MAQSGDAIVSLMRVRQSPQRLVADLARPWSLLKANSHLVSVS"
     gene            complement(4198..>4512)
                     /locus_tag="PLO65_10650"
     CDS             complement(4198..>4512)
                     /locus_tag="PLO65_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28746.1"
                     /translation="LVAGSRLPAYDASKAALGGLMRHVALEGSRKGVRANIVAPGLVD
                     TPLGRRATQGRPSRGTAPQFFGRQATGWEIAYAALFFMSDEAVYVTGQTLAVDSGLTG
                     IS"
BASE COUNT          739 a         1505 c         1388 g          686 t
ORIGIN      
        1 cgccctggcc cggctgctcg acggcgtgct gctggaccta gctccggtgg cgctggacgc
       61 cggcttcctc ggtccccgcg ccgccgactg gctcggggcg gcggccaagg gtgggcccga
      121 agcccccctc gccttccaca tggacccgct gtcggccttc gccgaggccg gcgtctcccc
      181 cggaccggtc gagagccata tcgtctcggc cgccaccgtc ggggcccggc tcnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaggg
      361 cgctggtacg ggccggtctg acgatggagg acgccttcgg gcgcatcgcc ttgggcgtct
      421 ccatcgacgg cgaatatttc accggcgtgg ccaaggtccg cgccgtgcgg gcgctgtggg
      481 cccggattac cggcgcctgc ggcgtcgcga tcccggcgcg tatcgaggcc cggtcgtcgc
      541 ggcgcatgct gacggcgctc gatccctgga ccaacctgat ccgcctgacg gccgccggct
      601 tcgcggccgc ggtcggcggc gccaacacca tcgttctggg cgccttcacc gacgcgctgg
      661 gcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnggccg gcggcgcctg gtatctcgac cagctgaccg acgacctcgc
      781 ccgcgccgcc tggacccgct tccaggccat cgaggccggc ggcgggatcg tcgccgctct
      841 ctcttcgggt gtgatcgccg cggatgtcgc cgccagtcgt caggccacgg agacggccgt
      901 ccgcgagggc ggggtcaagc tgctcggggt caccgtgttc cgcaacgccg acgcccggcc
      961 ggtcgaggtc gaggcggcgg agccggtcgc cgtgtccggc ccggagatcc ggctgcccgg
     1021 gccggacagc ctctgtccgc cgctggctcc ggcccgcctg tccgccgcct tcgaggaggc
     1081 ctgatccatg gccagcttcc ccaacttcgc cgaactcgcc ttcgaagagg ccgcgacctc
     1141 ggccccggcc gccgaaggcc atgtctggga aacgccggaa ggcatacccg tgtccgcggc
     1201 ctatgacgcc gacgacgtgg ccggcctgga tttcaccggc ggctatccgg gcatcgagcc
     1261 gttcgttcgc ggcccctacc cgaccatgta cgcgaccaat ccctggacgg tgcggcagta
     1321 cgcgggcttc tcgaccgccg aggattccaa cgccttctat cgccgcaacc tggcggccgg
     1381 gcagatgggc ctgtcggtgg ccttcgacct ggccacccac cgcggctacg acagcgacca
     1441 tgagcgggtg aagggcgacg tcggcatggc cggcgtggcg atcgacagca tccttgacat
     1501 gcgcacgctg ttcgacggca tcccgctcga caagatgagc gtgtcgatga ccatgaacgg
     1561 cgcggtgctg ccgatcctgg cgctctacat cgtagccgcc gaggaacagg gcgttccgcc
     1621 ggagaagctc tccggcacga tccagaacga cattctcaag gagttcatgg tccggaacac
     1681 ctacatctat ccgcccttgc cctcgatgcg gatcatttca gacatctttt catacacttc
     1741 gagcaagatg ccgaagttca actcgatctc gatttccggc taccacatgc aggaagccgg
     1801 ggcgacggcg gacctcgagc tggcctacac cctggctgac gggatcgagt acgtccgggc
     1861 cggcatcgcc gccgggatga ccgtcgacca gttcgcgccg cgcctgtcgt tcttctgggc
     1921 catcggcatg aactacttca tggaagtggc caagatgcgc gccgcgcgcc tgctctgggc
     1981 gcggttgatg aagcgggagt tcgacccgaa ggacagtcgc tcgctcagcc tgcgcaccca
     2041 cagccagacc agcggctggt cgctggcggc ccaggatgtg ttcaacaacg tcgcccgcac
     2101 ctgcgtcgag gccatggccg cggtcaacgg ccagacccag agcctgcaca ccaacagcct
     2161 cgacgaggcc ctggccctgc cgaccgactt ctcggcccgc atcagccgca acacccagct
     2221 gttcctgcag atggagagcg gcacgacccg cgtcgccgac ccctggggcg gcagctacta
     2281 cgtcgagcgg ctgacgcatg atctggccgc ccgggcgctg gagcatatcg acgaggtcga
     2341 aaagctcggc ggcatggcca aggccattga gcaggggctg cccaagctgc gcatcgagga
     2401 ggccgccgcc cgcacccagg cgcgcatcga cagcggccgc cagagcgtgg ttggggtcaa
     2461 tcgctacaag cccgaggtcg ccgacgacat cccggtgctc aaggtcgata acacggccgt
     2521 gcgcaacgcc cagctggaaa aactgaagag actcaagtcc gaacgcgacg aagctgaaac
     2581 gctggctgcg ctcgccgcgc tggaagccgg ggcccgcgcc gacggcaacc tgctggagct
     2641 ggccgtcaac gccgcccggg ccaaggccac cgtcggcgag atcagctatg ctctggaaaa
     2701 ggtcttcggc cgccaccggg cggacatcaa gtcaatccag ggagtctatg tgtccgaggc
     2761 cggatccaac gccgccgtcg cccgcgccaa ggccatggtc gaggccttcg aacaggccga
     2821 cggccgccgc ccgcgcatcc tgatcgccaa gatgggacag gacggccacg accgcggcca
     2881 gaaggtcatc gccacggcct tcgccgacct cggcttcgac gtcgacatcg gccccctgtt
     2941 ccagaccccc gccgaggccg cacgccaggc ggtggagaac gacgtgcatg tggtcggggc
     3001 gtcatcgctg gccgccggcc acctgaccct ggccccggag ctgatcgccg aactgggcaa
     3061 ggccggccgg ccggacatca tgaccgtcat cggcggcgtg atcccgccgc aggacttcca
     3121 ggccctgacg gacgccgggg tggtggccat cttcccgccc ggcaccgtga tcgccgaggc
     3181 ggccatcgac ctgctggaga agctgaaccg gcagttgggg tacgatcagg cgtagagagg
     3241 tcgctcggat gaagttccgc ccgttcacca tgccagatga aataggtagc gagccggacc
     3301 cagacctcga gcagtacttt acgctctaca gagatgagct cttcgacggc ttcgtgggcc
     3361 acggcctcgt gagcattcgt caggactggc cgcctgatct aatcggcaaa attgccgctg
     3421 ccgtggtttc aaataacctc gggataggtc tcgatcatgc ctacaagcgg tactgtcccc
     3481 ccgaaaacta taggcggaat gatgatccag aagtgccgaa gctggacgca cagttcgaac
     3541 gcgatttcga caacctgtgc tttgcgctga acgagagcta tgcttttgaa aaggcggacg
     3601 atgcaccgga gcaggacttc ttttccgcct tcggcatcct tcgagcgcga ggcatgctgg
     3661 acgccgccca cgccctggcc caacagggct atctttccga aacgctaatc ttggtacgcg
     3721 gctcgctgga aattcttgcc tgggccgcgg aagcaagcgt ttttcaagaa gactatcgac
     3781 cgctcgaact gacgacccag tttgccatta ccaaatcgaa ggccacgatc ccgggtctgg
     3841 cacaaatcta tggcaccctc tcaaaattcg cacactggca tccggacaca cacctcgcct
     3901 tcctgctggg aagaagcggg agaaccgccg tccgtcaaag gtcggtggaa cacaaggcag
     3961 ctgcgctgtt ggctgtcgca tccggccaac tgacggttgc gattaccttc ttggcgaaca
     4021 tgcagcgctg gtacccgacc catgacatgt ctgatgtacg ggcggcagct agccaaatgg
     4081 cgcagtcagg agacgcaatc gtctcgttga tgcgcgtacg gcagtcgcct caaaggctcg
     4141 tggccgatct cgcgcgacca tggagccttt tgaaggccaa cagccatctg gtctcagtta
     4201 gctgatcccc gtcagccctg aatccaccgc cagggtctgg cccgtcacat agaccgcttc
     4261 atccgacatg aagaacagcg ccgcataggc gatttcccag ccggtggcct ggcggccgaa
     4321 gaactgcggc gcggttccgc ggctcgggcg accctgggtg gcgcggcggc cgagcggggt
     4381 gtcgaccagg ccgggcgcca cgatgttggc ccgaacgccc ttgcggctgc cttccagcgc
     4441 cacatgccgc atcagcccgc ccagcgccgc cttcgaggcg tcgtaggccg gcaggcgcga
     4501 gcccgcgaca ag
//
LOCUS       DAORNX010000235         4504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14339_length_4504_cov_6.506631, whole genome shotgun sequence.
ACCESSION   DAORNX010000235 DAORNX010000000
VERSION     DAORNX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14339_length_4504_cov_6.506631"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..953)
                     /locus_tag="PLO65_10655"
     CDS             complement(168..953)
                     /locus_tag="PLO65_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28747.1"
                     /translation="MSTATTEQALLACIRVPKSGSKSLSRIVAEAFPDRARFYLPDTL
                     RREALISRWQAFRLWRSQTQNLSRHWRVLSLPAALAKIDAAAGPGDLLIGGHFDQPTV
                     QAGLSRPVRSIVLLRDPAERARSDYNYARQGYLKKKPWQRFDAHLTAQAAGRYDFEGF
                     LDYQLEHPAVFGDLACAFVGWREGAPIESLTPTIWCWGVLERSEAFAGRMSQLVGRSL
                     TFGVSNATRQVEQLDISVAARRRIERLYARDLELYARCRQDYG"
     gene            complement(953..1780)
                     /locus_tag="PLO65_10660"
     CDS             complement(953..1780)
                     /locus_tag="PLO65_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRD28748.1"
                     /translation="MTPDDIKLIVALCRQRAGLNIDPTRTYLIESRLAPLARREAYGS
                     VSELIAGLRARREEPLIWALVESMCSSETAFFRDRTPFQQFRDEILPTLSRMRGPEPI
                     RIWSAGCSTGQEAYSLAMLAEAGEGLEPGTRIEIYGSDLSERCLEKAQAGIYNQFEVQ
                     RGLPIRLLVDHFDKVEDSWRLSPRIRGRVRWRRINLAADLSAVGRFDIIFCRNVLSAM
                     DGATRPRVLEGLAGALPADGFLVLGKDESPLGLTDAFRPITGREALFARNPAFRVAA"
     gene            complement(1777..2142)
                     /locus_tag="PLO65_10665"
     CDS             complement(1777..2142)
                     /locus_tag="PLO65_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28749.1"
                     /translation="MKTCLLVDDSRVIRKVARRIVEELGYEVAEASDGLEALAWCRAA
                     MPDVILLDWNMPVMNGLDFLRQLRSEPGGDAPRVVFCTVETDPARIREALAAGACEYI
                     MKPFDGDIIQAKFAEAGLA"
     gene            complement(2421..3101)
                     /locus_tag="PLO65_10670"
     CDS             complement(2421..3101)
                     /locus_tag="PLO65_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HRD28750.1"
                     /translation="MTDPDPTDRPPPAPEDLLPEAAELAAQIAARLCHDFISPASAIV
                     SGLDLLEDPTAQDMREDAMDLIGQSARKLADLLQFSRVAFGASASAETFDPRELETLA
                     RGIYAHVRPELDWKVEAASLNKAAARILLNLCQIAAGALPIGGTAVARVAVADGRAAI
                     SLEATGPRARLKPEVLRGLRGEPRGDAVGGAWVQAYYLHLIVKAAGGQLAAESGEDQV
                     TLAAWAPA"
     gene            3167..3355
                     /locus_tag="PLO65_10675"
     CDS             3167..3355
                     /locus_tag="PLO65_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HRD28751.1"
                     /translation="MDPFIEPGALVRHPDREDWGLGQVQSVTGRRVTVNFDNAGKQAI
                     DASVVRLVFVADDPGARR"
     gene            3352..4125
                     /gene="cysQ"
                     /locus_tag="PLO65_10680"
     CDS             3352..4125
                     /gene="cysQ"
                     /locus_tag="PLO65_10680"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRD28752.1"
                     /translation="MSDADTGLILADIVEEAGRLILPYWSHGGTVAHTSKADMSPVTI
                     ADQEGEKLIVARLKERFPDIPVVAEEHASEFGTPDAIGDRFFLVDPVDGTKAFMRGDP
                     AFTVNIGLVAGRRPVAGAVCAPASGETWYTTAAGAMKRPTDGGAASPVHVRPWPKGAA
                     IALISHTMKPETADRLAAEYGFDLRVAMDSSVKLCRIAEGAADIYPRHGPTSEWDICA
                     AQAVLEAAGGSVTGYQGADFLYGKADKRFLNEWFVARGG"
     gene            complement(4129..>4504)
                     /locus_tag="PLO65_10685"
     CDS             complement(4129..>4504)
                     /locus_tag="PLO65_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRD28753.1"
                     /translation="LFLGDGHALQGDGEITGQGLETSLDVTFRVELIRGQTLGQVWME
                     NDEYVMVSGIDNGLDAALASATAGLARWLRDHYGLADSEIAALLGAAVKYDIAEIVDS
                     RPHVVARISKAVLATLPAAGTK"
BASE COUNT          682 a         1564 c         1618 g          640 t
ORIGIN      
        1 ttccagccgc tccagcggcg gcatgaacac atagagatga ccgtcgcgcg gttcgaggga
       61 acctggaagc tgtcgcagaa caagccggcg gaggcggtcg cgcgggtcgc ctcggcgctg
      121 gagcgccgtc cggacgccgc ggcgcgcgat gtcgcgcggc ggatgcgtca gccgtagtcc
      181 tggcggcagc gggcgtagag ctccaggtcc cgggcgtaga gccgctcgat ccgccgccgc
      241 gccgccaccg agatgtcgag ctgctcgacc tggcgggtgg cgttggagac gccgaaggtc
      301 aacgaccggc cgaccagctg cgacatgcgc ccggcgaagg cctccgaccg ctccagcacg
      361 ccccagcacc agatcgtcgg cgtcaggctc tcgatcggcg cgccctcgcg ccagccgacg
      421 aaggcgcagg ccaggtcgcc gaacaccgcc gggtgctcga gctggtagtc gaggaagccc
      481 tcgaagtcat agcggccggc ggcctgggcc gtcaggtgcg cgtcgaaccg ctgccagggc
      541 ttcttcttca gatagccctg ccgggcgtag ttgtagtccg accgggcccg ctcggccggg
      601 tcgcgcagca gcacgatgct cctgaccggc cgggacaggc cggcctggac ggtgggctgg
      661 tcgaaatgac cgccgatcag caggtcgccg ggcccggcgg ccgcgtcgat cttcgccagg
      721 gccgcgggca gggacagcac ccgccagtgt ctagacaggt tctgggtctg ggagcgccac
      781 agacggaagg cctgccagcg gctgatcagc gcctcgcgtc gcagggtgtc gggcaggtag
      841 aagcgggcgc ggtcgggaaa ggcctcggcg acgatgcgcg acaggctctt ggagccgctc
      901 ttgggcaccc ggatgcaggc cagcagggcc tgctccgtgg tcgcggtcga catcaggcgg
      961 cgacccggaa ggccggattg cgggcgaaca gcgcctcgcg gccggtgatc ggccggaagg
     1021 cgtcggtcag gccgagcggg ctttcatcct tgcccagcac gaggaagccg tcggccggca
     1081 gggccccggc gaggccctcc aacacccgcg gccgggtcgc cccgtccatc gccgacagca
     1141 cgttgcggca gaaaatgatg tcgaaccggc cgaccgccga caggtcggcc gccaggttga
     1201 tccggcgcca gcgcacccgc ccgcgaatcc gcggcgacag ccgccagctg tcctcgacct
     1261 tgtcgaaatg atcgaccagc agccggatcg gcaggccccg ctggacctcg aactggttgt
     1321 agatgcccgc ctgggccttc tcgaggcagc gttccgacag gtcggagccg tagatctcga
     1381 tacgggttcc gggctccagc ccctcgccgg cctcggccag catggccagg ctgtaggcct
     1441 cctggccggt cgagcagccg gcgctccaga tgcggatcgg ctcggggccg cgcatgcgcg
     1501 acagggtcgg caggatctcg tcgcggaact gctggaacgg cgtgcgatcg cggaagaagg
     1561 cggtctccga cgagcacatc gactcgacca gggcccagat cagcggctcc tcgcggcgcg
     1621 cgcgcaggcc ggcgatcagt tcgctcacgg agccgtaggc ctcgcgccgg gccaacggcg
     1681 ccaggcggct ctcgatcagg taggtgcggg tcggatcgat gttcaggccc gcgcgctggc
     1741 ggcaaagggc gacgatgagc ttgatgtcgt ccggcgtcat gccaggcccg cctcggcgaa
     1801 cttcgcctgg atgatgtcgc cgtcgaaggg cttcatgatg tattcgcagg cgcccgcggc
     1861 aagggcctcg cgaatgcgcg caggatcggt ctcgacggtg cagaacacca cccggggcgc
     1921 atcgcccccg ggctcggagc gcagctggcg caggaaatcc aggccgttca tcaccggcat
     1981 gttccagtcg agcaggatga cgtcgggcat ggccgcccga caccaggcga gcgcctccag
     2041 tccgtcggac gcctcggcca cctcgtagcc gagctcctcg acgatccggc gggcgacctt
     2101 gcggatcacc cggctgtcat caaccagtag acaggttttc aaggcgcgag cgctccttcc
     2161 ccgtccgagt tgtcgccggt gtcggttaac gggtcgtgag aggacgcagt gaagatcggg
     2221 aatatcggcg ctccccaccc ttcgtgggga gctgtcaggc ggagcctgac tgaggggcgg
     2281 cgccggaggc ggcgatcccg gcctgaacgc cgatcctggc cgccagtcgt attcagggct
     2341 ttcccgcgcc gctggcgcag ccccaacacc acgctccgcg cggtccgcct ccccgcgatg
     2401 cggggaggaa tgtctggatc ctacgccggc gcccaggccg ccagggtgac ctggtcctcg
     2461 ccgctctcgg cggcgagctg gccgccggcg gccttgacga tcaggtgcag atagtaggcc
     2521 tggacccagg ctccgccgac cgcgtcgccg cgcggttcgc ccctgaggcc gcgcagcacc
     2581 tcgggcttga gcctggcgcg gggcccggtc gcctcgaggg aaatcgcggc gcggccgtcg
     2641 gccacggcca cccgggccac ggccgttccg ccgatcggca gggcgccggc ggcgatctga
     2701 cacaggttga gcaggatgcg ggcggcggcc ttgttcagcg acgccgcctc gaccttccag
     2761 tcgagctccg gccggacgtg ggcgtagatg ccccgcgcaa gggtctccag ctcgcgcggg
     2821 tcgaaggtct ccgccgaggc cgaggcgccg aaggcgacgc ggctgaattg cagcaggtcg
     2881 gccagcttgc gggcgctctg gccgatcagg tccatggcgt cctcgcgcat gtcctgggcc
     2941 gtcgggtctt ccagcagatc caggcccgag acgatggcgc tggccgggct gatgaagtcg
     3001 tggcacagcc gcgccgcgat ctgggcggcc agctcggcgg cctcgggcag caggtcctcg
     3061 ggggcgggcg gcgggcggtc ggtggggtcg ggatcggtca tggccggcga aattgacctg
     3121 atttgaagcg ttggcaaatg gggccggggc ccctaacgaa gcggccatgg accccttcat
     3181 cgagcccggc gccctggtgc ggcatcccga tcgcgaggac tggggtctgg ggcaggtgca
     3241 gtcggtgacc ggtcggcgcg tcaccgtcaa tttcgacaac gccggcaaac aggccatcga
     3301 tgcgtcggtc gtgcgcctgg ttttcgtggc cgacgatcct ggagcccgcc gatgagcgac
     3361 gctgatactg gactcatcct ggcggacatt gtcgaggagg ccggacgcct gatcctgccc
     3421 tactggtcgc acggcggcac cgtggcccac accagcaagg ccgacatgag cccggtgacc
     3481 atcgccgacc aggagggcga aaagctgatc gtcgcccgcc tgaaggaacg ctttcccgac
     3541 atccccgtcg tcgccgagga gcatgccagc gagttcggca cgccagacgc catcggcgat
     3601 cgcttcttcc tcgtcgaccc ggtcgacggc accaaggcct tcatgcgcgg cgacccggcc
     3661 ttcacggtca acatcggcct ggtggccggc cgccgcccgg tcgccggcgc ggtctgcgcc
     3721 cccgcctcgg gcgagacctg gtacacgacc gccgccgggg cgatgaagcg cccgaccgac
     3781 ggcggagcgg cgtcaccggt gcacgtgcgg ccctggccga agggcgccgc gatcgccctg
     3841 atcagccata cgatgaagcc ggagaccgcc gacaggctgg ccgccgaata cggcttcgac
     3901 ctgcgggtgg cgatggacag ctcggtcaag ctgtgccgca tcgccgaggg cgccgccgac
     3961 atctatccgc gccacggtcc gacctccgag tgggacatct gcgccgccca ggcggtgctg
     4021 gaggccgccg gcggatcggt aaccggctac cagggcgccg acttcctcta tggcaaggcc
     4081 gacaagcgct ttctcaacga gtggttcgtc gcccgcgggg gataggctct acttcgttcc
     4141 ggccgcggga agcgtcgcca gcaccgcctt cgagatccgc gcgaccacat ggggccggga
     4201 atcgactatc tcggcgatgt cgtacttcac cgccgcgccc agcaaggcgg cgatttcgga
     4261 atcggccagg ccgtagtgat ccctgagcca gcgggccagg ccggcggtgg ccgaggccag
     4321 ggccgcgtcg agtccgttgt cgatgcccga caccattacg tactcatcgt tctccatcca
     4381 gacctggccg agagtctggc cccggatcag ctcgacgcgg aaggtcacgt ccagggaggt
     4441 ctccaggccc tgaccggtga tttcaccatc gccctgcagg gcgtggccgt cgccgaggaa
     4501 cagc
//
LOCUS       DAORNX010000236         4495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14370_length_4495_cov_6.096847, whole genome shotgun sequence.
ACCESSION   DAORNX010000236 DAORNX010000000
VERSION     DAORNX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_4495_cov_6.096847"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>207
                     /locus_tag="PLO65_10690"
     CDS             <1..>207
                     /locus_tag="PLO65_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD28754.1"
                     /translation="RAADAAVARGERRPLLGVPMTVKEANWVAGLETTWGMEMWRDWK
                     APVDGVGIARLKAAGAVILGKSNVP"
     assembly_gap    208..454
                     /estimated_length=247
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <455..1211
                     /locus_tag="PLO65_10695"
     CDS             <455..1211
                     /locus_tag="PLO65_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD28755.1"
                     /translation="GVEGVDVPVAVVGPMARTAADLDLALGVLAGPDDSEAVGYSLAF
                     PPPRRQRLADYRVLILDHHPLAATDNEIGAALNGLGDRLEALGARVDRQTELLPDLVD
                     AQEVYGAILNTAMTMGQPGARPPSAHEWMTLLTRQLMIRRQWARLFEGFDVMLAPALG
                     TLAFPHDDSPDMGARTLLLNGEKTPYFSQIAWSGLATLGHLPATAAPIGLSAGGLPIG
                     IQIIGPYLEDRTTIAFAGLLEREFGGFVAPPGF"
     gene            complement(1291..2052)
                     /locus_tag="PLO65_10700"
     CDS             complement(1291..2052)
                     /locus_tag="PLO65_10700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRD28756.1"
                     /translation="MAWTRTYDEAEPQRVNRWLAQSGVCSRREAEALIGRGLVSIDGE
                     VVGDAGRKILPGQTLTLADAATGALSSSLTLMFHKPVGIVSSQPEGDQVPAVRLITRK
                     TVWGPCTIFPDRDSRLAPVGRLDMDSRGLLILSEDGVVAKAVIGPDSGLEKEYRVQVA
                     GQITQDKIERLRYGLMLDGRQLKQAQVNLQGKQTLSFVLKEGRNRQIRRMCDLVGLEV
                     VDLLRTRIGPITLGALPEGRWRPLAPEERAALIKG"
     gene            complement(2070..2465)
                     /locus_tag="PLO65_10705"
     CDS             complement(2070..2465)
                     /locus_tag="PLO65_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD28757.1"
                     /translation="MESLASSKAGLWTGRVMSTLFVLFLLMDSVIKLIPIQPVITTMT
                     EMQLPVSMARPIGIIELASLALYLWPRTAVLGAVLFMGVFGGAIASHWRLGDPLFSHT
                     LFGVYLDILMWGGLWLRDAALRSLFPYRR"
     gene            complement(2488..2892)
                     /locus_tag="PLO65_10710"
     CDS             complement(2488..2892)
                     /locus_tag="PLO65_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD28758.1"
                     /translation="MTDQAPPTKGVIPYLNVVGAAEAADLYRAAFGAIEHRRMPADDG
                     KRLMHCHLEINGGSLMLSDCFPESGYEHQPSHSFTMQLVVDDIDAWFQRAVDAGLTVT
                     NPVTLMFWGDRWGSLTDRFGVHWAMNQPTEQA"
     gene            complement(2909..3373)
                     /locus_tag="PLO65_10715"
     CDS             complement(2909..3373)
                     /locus_tag="PLO65_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD28759.1"
                     /translation="MTVAADHELVLEREFDAPPEAVYRCWTDPDLIPRWFVPRPWTIS
                     KVEQDLRPGGSSSVTMKDPEGNEFPNPGVYLEVVPNRRLVFTDAFVGDWVPAGPFMVA
                     IVEFAPLEGGRTRYRATARHWTAEAKAQHEQMGFLEGWGQCADQLAELLATL"
     gene            complement(3455..>4495)
                     /locus_tag="PLO65_10720"
     CDS             complement(3455..>4495)
                     /locus_tag="PLO65_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRD28760.1"
                     /translation="GARRRAKQTALPDEAVLSDIDDAETPLAERLDGADYRDDVLRLM
                     FICCHPDLPATQQIALALRIVSGLSVKQIARAFLVGESAMEQRITRAKAKIAGAGVAF
                     ETPGPAERSERVAAVAAMIYLVFNEGYSAGGRQDDRARLADEAIRLARLLLRLYQTEP
                     EIMGLTALLLLQHARAAARFDAGGEIVLLDDQDRSLWDRRLIGEGLALVDKAMRHRRP
                     GPYQIQAAIAALHARAARAEDTDWAGIAALYAALERQTPSPVITLNRAVAVSRTEGAE
                     AALDLIAPLEARLSGYFHFYGVRGALLLDLGREAEARTAFNQAIALANTPAEAAHIRR
                     HLDRLRPSDEVH"
BASE COUNT          623 a         1488 c         1496 g          641 t
ORIGIN      
        1 agggccgccg acgccgctgt cgcccggggc gagcgccggc cgctgctcgg ggtgccgatg
       61 acggtcaagg aggccaactg ggtcgccggc ctcgagacca cctggggcat ggagatgtgg
      121 cgcgactgga aggcccccgt tgacggggtc ggcatcgccc gcctgaaggc cgccggggcg
      181 gtgatccttg gcaagagtaa cgtgccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggggt cgagggggtt gacgtgccgg
      481 tggccgtggt cgggccgatg gcccgcacgg cggccgacct cgacctggcc ctgggcgtgc
      541 tcgccggacc cgacgacagc gaggccgtcg gctacagcct ggccttcccg ccgccgcggc
      601 gccagcggct ggccgactac cgggtgctga tcctcgacca ccatccgctg gcggccaccg
      661 acaacgagat cggcgcggcc ctgaacggcc ttggcgaccg cctcgaggcg cttggcgcac
      721 gcgtcgaccg gcagaccgag ctgctgcccg atctcgtcga cgcccaggag gtctacgggg
      781 ccatcctcaa cacggccatg accatgggcc agcctggcgc ccggccgccc agcgcccatg
      841 aatggatgac cctgctgacg cgccagctga tgatccgccg ccagtgggcg aggctgttcg
      901 aggggttcga cgtgatgctc gcgccggcgc tcggaaccct ggccttcccg cacgacgaca
      961 gccccgacat gggcgcccgg accctgctgc tgaacggcga gaagaccccc tatttcagcc
     1021 agatcgcctg gtccgggctg gccaccctcg ggcacctgcc ggccaccgcc gcgccgatcg
     1081 ggctgagcgc gggcggactg ccgatcggaa tccagatcat cggcccctat ctcgaggacc
     1141 gcacgaccat cgccttcgcg ggtctgctgg agcgagagtt cggcgggttc gtggcgccgc
     1201 cggggttcta ggggacatgt accgccttcg gtacgtgtcc ccgaaattcg caggggtctg
     1261 acggggacac gtaccttcgg tacatgtccc ctaacccttg atcagcgccg ctctttcctc
     1321 cggcgccagc ggccgccagc ggccttcggg cagggcgccc agggtgatgg ggccgatgcg
     1381 ggtgcgcagc aggtcgacga cctccagccc gaccaggtcg cacatccggc gaatctggcg
     1441 gttgcggccc tccttgagca cgaaactgag ggtttgcttg ccctgcaggt tgacctgcgc
     1501 ctgcttcagc tgccgtccgt cgagcatcag gccgtagcgg agccgctcga tcttgtcctg
     1561 ggtgatctga ccggcgacct ggacgcggta ttccttctcc aggccggagt cggggccgat
     1621 caccgccttg gcgaccaccc cgtcctccga caggatcagc aggccgcgac tgtccatgtc
     1681 gagccggcca accggcgcca ggcggctgtc gcggtcggga aagatggtgc acggacccca
     1741 gacggtcttg cgggtgatca gccggacggc cggaacctgg tcgccctcgg gctgggacga
     1801 cacgatcccc accggcttgt ggaacatcag cgtcaggctc gacgacaggg cgccggtggc
     1861 cgcatcggcg agggtcaggg tctggccggg caggatcttg cgcccggcgt ccccgaccac
     1921 ctcgccgtcg atgctgacca gccccctgcc gatcagcgcc tcggcctcgc gccgtgaaca
     1981 gacgccgctc tgcgccagcc aacggttgac ccgctggggc tcggcttcgt cataggtgcg
     2041 ggtccaggcc atgaggcctg actaggcgat cagcgccggt acgggaagag cgaccgcagg
     2101 gcggcgtcgc gcagccacag accgccccac atcaggatgt ccagatagac cccgaacagg
     2161 gtgtggctga acagcgggtc gccgagccgc cagtggctgg cgatggcccc accgaacacg
     2221 cccatgaaca gcacagcgcc cagcacggcc gtacgcggcc agagatacag cgccaggctg
     2281 gccagttcga tgatcccgat cggccgggcc atgctcaccg gcagctgcat ctcggtcatg
     2341 gtcgtgatga ccggctggat ggggatcagc ttgatcacac tgtccatcag caggaacagc
     2401 acgaacaggg tgctcatcac ccggccggtc cacaggcccg ccttcgagct cgcgaggctt
     2461 tccattgtcg ttcccccttc tggtcgctca ggcctgctcc gtgggctggt tcatcgccca
     2521 gtggacgccg aaccggtccg tcagcgagcc ccaacggtcg ccccagaaca tcagcgtcac
     2581 cggattggtc accgtcaggc cggcatcgac ggcccgctgg aaccaggcgt cgatgtcatc
     2641 gaccaccagc tgcatcgtga agctgtgcga gggctggtgc tcgtagccgc tctcggggaa
     2701 acagtcgctg agcatcagcg agccgccgtt gatctccaga tggcagtgca tcagccgctt
     2761 gccgtcgtcg gccggcatcc ggcgatgctc gatcgcgccg aaggccgccc tgtagaggtc
     2821 cgccgcctcc gcggcgccga cgacgttgag ataggggatc acgcccttcg tgggcggtgc
     2881 ttgatcggtc attacggtgg tcccttcgtc agagcgtggc gagcagttcg gcgagctggt
     2941 cggcgcactg gccccaaccc tcgagaaagc ccatctgctc gtgctgggcc ttggcttccg
     3001 ccgtccagtg gcgggcggtg gccctgtacc gggtgcggcc gccctcgagg ggcgcgaatt
     3061 cgacgatggc gaccatgaac ggcccggccg gaacccagtc gccgacaaag gcgtcggtga
     3121 acaccagtcg gcggttcgga accacctcga ggtagacgcc ggggttcgga aactcgttgc
     3181 cctccgggtc cttcatggtc accgacgagg agccgcccgg gcgcaggtcc tgctcgacct
     3241 tcgagatcgt ccacggccgg ggaacgaacc agcgcgggat caggtcggga tcggtccagc
     3301 aacggtagac cgcctcgggc ggcgcatcga actcccgctc cagcacaagt tcatggtctg
     3361 cggcgacggt catcggcggt ctcccttctt ctggatggct ccgtccgaag gacgaacggg
     3421 ccgtcgcgac gccgacaggg ccggcgactt ttgttcagtg aacttcgtcg gacggcctca
     3481 gccggtcgag atgccgacgg atgtgggccg cctcggcggg ggtgttcgcc agggcgatgg
     3541 cctggttgaa cgccgtgcgg gcctcggcct cgcggcccag gtcgagcagc agggcgccgc
     3601 gcacgccata gaagtggaag tagccggaca ggcgcgcctc cagcggggcg atcaggtcca
     3661 gcgccgcctc cgccccctcg gtccggctga ccgccacggc gcggttgagc gtgatcaccg
     3721 gggagggggt ctgtcgctca agcgccgcgt agagggccgc gatgccggcc cagtcagtgt
     3781 cttcggccct cgccgcgcgg gcgtgcaggg cggcgatggc ggcctggatc tggtagggtc
     3841 ccggccggcg gtggcgcatg gccttgtcga ccagggccag accttcgccg atcagccggc
     3901 ggtcccacag cgatcggtcc tggtcgtcca gcagcacgat ctcgcccccg gcgtcgaacc
     3961 gggcggcggc gcgggcgtgc tgcagcagca gcagggcggt caggcccatg atctcgggct
     4021 cggtctggta gagccgcagc agcagccggg ccaggcggat ggcctcgtcg gccagccgcg
     4081 cgcggtcgtc ctgccgccca ccggccgaat agccctcgtt gaacaccagg tagatcatcg
     4141 ccgccacggc cgcgacgcgt tcggaccgct cggccgggcc gggggtttcg aaggccacgc
     4201 ccgcgccggc gatcttcgcc ttggcccggg tgatccgctg ctccattgcg ctttcgccca
     4261 ccaggaaggc gcgggcgatc tgcttcaccg acaggcccga gacgatgcgc agggccagcg
     4321 cgatctgctg ggtggccggc aggtccgggt ggcagcagat gaacatcagc cgcagcacgt
     4381 cgtcgcggta gtcggcgccg tcgagccgct cggccagcgg cgtctcggcg tcgtcgatgt
     4441 cggacagcac cgcctcgtcg ggcagggccg tctgcttcgc gcgccggcgg gcccc
//
LOCUS       DAORNX010000237         4479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14449_length_4479_cov_6.216094, whole genome shotgun sequence.
ACCESSION   DAORNX010000237 DAORNX010000000
VERSION     DAORNX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14449_length_4479_cov_6.216094"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="fliI"
                     /locus_tag="PLO65_10725"
     CDS             complement(<1..500)
                     /gene="fliI"
                     /locus_tag="PLO65_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518508.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HRD28761.1"
                     /translation="MRSLIAAVERIEPLSVSCRVAAVNGLLIEARGGLTHLAVGARAE
                     IERRNAPALAAEVVGFRETRALLMPFGPVEGIAPGAEIRIQPDGAMVRPHKAWLGRII
                     DAFGNPIDGKGPLPQGASPYSLRAPPPPAHARGRVGERLDLGVRSMNVFTTTCRGQRL
                     GIFAGSG"
     gene            complement(608..1009)
                     /locus_tag="PLO65_10730"
     CDS             complement(608..1009)
                     /locus_tag="PLO65_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28762.1"
                     /translation="MTQVSLPRLYALRAAYLLMSVGLGLTIWPGILHPEKPWPLMYGV
                     VQCVLAAVSLLALLGLRHPLKMLPLLLFEMIWKGLWLGLVGIPAWRAGGMSAGTAETL
                     FACGMGVIFPIVIPWGHVWATYVKAPGDRWR"
     gene            complement(1053..1280)
                     /locus_tag="PLO65_10735"
     CDS             complement(1053..1280)
                     /locus_tag="PLO65_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28763.1"
                     /translation="MSTPIYIFTALLPFVTALLIFGMRYVAQIQQAKARLAGDEAYHR
                     LAQKVATDQAAALAAIETRLAAIEKILRDVG"
     gene            complement(1292..1624)
                     /locus_tag="PLO65_10740"
     CDS             complement(1292..1624)
                     /locus_tag="PLO65_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD28764.1"
                     /translation="MIENPPSPTGDQLLAMLAALASPHRLRIIAALRAGGRNYISRLA
                     RDLGISRPLLHLHLQKLEDAGLVTSQLELSADGKALNFFEVTDFALQLTPEAIVRATA
                     SLTSKPET"
     gene            complement(1762..2142)
                     /locus_tag="PLO65_10745"
     CDS             complement(1762..2142)
                     /locus_tag="PLO65_10745"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD28765.1"
                     /translation="MPTGSCLCGAVSFEVAGELPAPDACHCSRCRKTSGHFWASTDLP
                     ESAVTIRGAGQVTWYPSSEKVKRGFCSTCGSALFWKPVHHPWIAIAMGAFDAPTGTRL
                     GKHIFVADKGDYYDIGDGLPQNQS"
     gene            2269..3303
                     /locus_tag="PLO65_10750"
     CDS             2269..3303
                     /locus_tag="PLO65_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD28766.1"
                     /translation="MRPSPRFFSSSEAARRLGVSAKALRLYEQRGLVVPGRTPAGWRA
                     WSQDDLECAADIAALRALGFSLDQVARVVTGDPRGLEPALAAHQAVLEARFGELVATL
                     ETVRAMREDLARGQTPTVRDLVALAKSAGPPALAFDLPWPWDGERFELRDIRPLTWIV
                     GPLGSGKTRLAMRLAEALPGAVFLGLDRRVAGRDSIAATTKQAPALRGRIERALAWLV
                     EDGATPSDDLGVLLACVEAGGSSPLVIDMIEQGLDHPTQTALAAYLRRRGPGARPLFA
                     MTRSSAILDLAAVGVDEAILLCPANHSPPMLVSPHAGAPGHEAVATCLGSPRVRARTA
                     GMVAVRPAAA"
     gene            complement(3305..3796)
                     /locus_tag="PLO65_10755"
     CDS             complement(3305..3796)
                     /locus_tag="PLO65_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28767.1"
                     /translation="MGRTADYSHQSCGIAATLEVVGDPWTLLILRDAFRGVRRFEQWQ
                     ERLGVARNVLAARLKSLVAHGVLEPRRYSERPPRQEYLLTAKGQALRPVLLTMADWGN
                     RQVYGDQAPVRFIHDHCGHAFTPRLVCEHCGEGLIPGDLRQTQINEDAPTVGEMLALP
                     DKA"
     gene            4003..4437
                     /locus_tag="PLO65_10760"
     CDS             4003..4437
                     /locus_tag="PLO65_10760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD28768.1"
                     /translation="MIPPDGFEPFTRPSPLIDPWRPLYMLAAPDRVVLGLFVREAHTN
                     SRDTAHGGLLAAIADQAMGMSCAARLKARGVAITALWTSSLTVDYLDVARPGQWLAFD
                     TGFCRPGRTICHAEADITADGATVARARGAFRVSLDRTEAAG"
BASE COUNT          669 a         1522 c         1590 g          698 t
ORIGIN      
        1 cccgagccgg cgaagatgcc caggcgctgg ccgcggcagg tggtggtgaa gacgttcatc
       61 gaccggacgc ccaggtccag ccgttcgccg acccggccgc gggcgtgggc cggcggcggc
      121 ggcgcgcgca ggctgtaggg cgacgcgccc tggggcaggg ggcccttgcc atcgatcgga
      181 ttgccgaagg cgtcgatgat ccggccgagc caggccttgt gcggcctcac catggcgccg
      241 tcgggctgga tgcggatctc ggccccgggc gcgatgccct cgaccggacc gaacggcatc
      301 agcagggcgc gggtttcgcg aaagccgacc acctcggcgg ccagggcggg cgcattgcgg
      361 cgctcgatct cggcccgggc cccgaccgca agatgggtca ggccgccgcg ggcttcgatc
      421 agcaggccgt tcaccgccgc cacgcggcag gacacggaca gcggctcgat acgctcgacg
      481 gcggcgatca gactgcgcaa cgcgatggcc ccgggaaacc cacggcctca ctgtggcaag
      541 gttgctttaa ccataagtga aaagccgaag gggacatgta ccgccttcgg tacatgtccc
      601 cggcccgcta ccgccaccga tcgcccggcg ccttcacata ggtcgcccac acatggcccc
      661 aggggatcac gatcgggaag atcaccccca tgccgcaggc gaacagggtt tcggccgtgc
      721 cggcgctcat gccgccggcc cgccaggcgg ggatgccgac caggcccagc cacaggccct
      781 tccagatcat ctcgaacagc agcagcggca gcatcttcag cggatggcgc agtcccagca
      841 gcgccagcag cgacaccgcc gccagcacgc actggaccac gccgtacatc aacggccagg
      901 gcttttcggg gtgcaggatg cccggccaga tggtcaggcc gaggccgacc gacatcaaca
      961 gataggcggc ccggagggcg tagaggcgcg gcagcgagac ctgggtcatg ccggaaagtc
     1021 cttgcggtga tgtgaggggg agggggtggc ggtcagccga cgtccctgag gatcttctcg
     1081 atcgccgcca ggcgggtctc gatggcggcc agggcggcgg cctggtcggt ggcgaccttc
     1141 tgggcaagcc tgtgataggc ctcatcgcca gcgagccggg ccttcgcctg ctgaatctgg
     1201 gcgacatagc gcatgccgaa gatcagcagc gccgtcacga acggcagcag ggcggtgaag
     1261 atgtagatcg gcgtggacat cggggtcccc cttatgtctc gggtttgctg gtcagggagg
     1321 ccgtcgcgcg gacgatcgcc tccggtgtga gctgcagggc gaagtcggtg acttcgaaga
     1381 agttgagcgc cttgccgtcg gcggacagtt cgagctggct ggtgaccagc cccgcgtcct
     1441 cgagcttctg cagatgcagg tggagcaagg gccggctgat gcccaggtcg cgcgccagcc
     1501 ggctgatgta gttgcgcccg ccggcccgca gcgccgcgat gatccgcagc cggtgcgggc
     1561 tggccagagc cgccagcatg gccagcagct ggtctcccgt cggggatggc ggattctcta
     1621 tcatatgtca gaaatatctg acgcgtgtca gggagtcaag cggcggggac atgtgccgcc
     1681 ggggacatgt gccgaaggta cgtgttctct ggttccaggt cgctcgattg ggggacacgt
     1741 acccaagagg gtacatgtcc cctagctctg gttctgcggc aggccgtcgc cgatgtcgta
     1801 gtagtcgccc ttgtcggcga cgaagatgtg cttgcccagc cgggtcccgg tcggggcgtc
     1861 gaaggcgccc atggcgatcg cgatccacgg gtgatggacc ggcttccaga acagggccga
     1921 gccgcaggtc gaacagaacc cgcgcttcac cttctccgac gacgggtacc aggtgacctg
     1981 tccggccccg cggatcgtca cggccgactc cggcaggtcc gtcgaggccc agaagtgccc
     2041 cgacgtcttg cggcaccgcg agcagtgaca ggcgtccggc gccggcagtt cgccggccac
     2101 ctcgaacgac acggcgccac agaggcagga tccggtaggc atggcggagg ctcccgagcg
     2161 ttcgtggatc ggcaggctcg cagatatccc cggggcgatc cagatgcttg acccggcccc
     2221 tgggttcgcc tcgatagacg ggctggcctg ccagcagcga ggtgagtcat gagaccttct
     2281 ccccggtttt tcagttcgtc cgaggccgct cgccggctcg gcgtgtcggc caaggccctt
     2341 cgcctgtacg aacagcgcgg actggtcgtt ccgggccgga ccccggccgg ctggcgggcc
     2401 tggagtcagg atgatctcga atgcgccgcc gacatcgccg ccttgcgggc gctgggattc
     2461 agcctcgacc aggtggcgcg ggtcgtcacc ggcgacccaa gggggctgga gccggcgctg
     2521 gccgcccatc aggccgtgct tgaggcccgc tttggcgagc ttgtcgcgac cctggagacc
     2581 gtccgcgcca tgcgcgagga cctggcgcgc ggccagacgc cgaccgtgcg cgacctggtc
     2641 gccctggcga agtccgccgg tccgcccgcc ctggccttcg acctgccctg gccgtgggat
     2701 ggcgagcggt tcgagctccg cgacatccgg cccctgacct ggatcgtcgg gccgctgggc
     2761 agcggcaaga cgcggctggc gatgcgcttg gccgaggcct tgcctggcgc tgtgttcctc
     2821 ggtctcgacc gacgggtcgc tgggcgggac tccatcgccg cgacgacgaa acaggcgcct
     2881 gcgctgcgtg gccggatcga gcgggcgctg gcctggctgg tcgaggacgg ggccacgccg
     2941 tcggacgacc tgggcgtgct tctggcctgt gtggaggcag gcgggtcgtc gccgctggtg
     3001 atcgacatga tcgagcaggg gctggatcac ccgacccaga cggccctggc cgcctatttg
     3061 cgtcgacggg ggccgggggc gcggccgttg ttcgcgatga cccgttcctc ggcgatcctc
     3121 gatctcgccg cggttggcgt cgacgaggcg atcctgctct gcccggccaa tcacagtccg
     3181 ccgatgctgg tgtcgccgca cgcgggagcg cctggccacg aggccgtggc cacctgcctg
     3241 ggctcgccca gggtccgggc ccgcacggcg ggaatggtgg ccgtccggcc ggcggcggcc
     3301 tgagtcaggc cttgtcgggc aacgccagca tctcgccgac ggtcggggcg tcctcattga
     3361 tctgcgtctg acgcaggtcg ccggggatca ggccctcgcc gcaatgctcg cagaccagcc
     3421 tgggggtgaa ggcgtggccg caatgatcat ggatgaaccg taccggcgcc tggtcgccat
     3481 agacctgccg gttgccccag tcggccatgg tcagcagcac cggtcgcagg gcctgaccct
     3541 ttgccgtcag caggtactcc tggcgcggcg gacgttcgga atagcgccgg ggctccagca
     3601 cgccatgcgc caccaggctc ttgagccgcg cggccagcac gttgcgcgcc acgcccagcc
     3661 gctcctgcca ctgctcgaag cggcgcacgc cgcgaaatgc gtcgcgcagg atcaacaggg
     3721 tccagggatc gccgacgacc tcaagggtgg cggcgatgcc gcagctctgg tgggaataat
     3781 ctgctgtccg gcccatggtt ccagagtgga gtttccccat agggaaagca agagggttgt
     3841 ctttcgcgac gaacctgaat aggttgcgtt tagcaacgca cttcctgact tgcctcctgg
     3901 agacggcgcc tcggaacgcc gacgccgtct cccctttttc cgcggcgctt gatatttcaa
     3961 acaatcgttt caaactatcg tttgcaacga ggagcgcccg ccatgatccc acccgatggg
     4021 ttcgagccgt tcacccgtcc cagtccgctg atcgatccct ggcggccgct ctatatgctg
     4081 gcggctcccg atcgggtggt gctgggattg ttcgtccgcg aggcgcacac caactcgcgg
     4141 gacaccgctc acggcggcct tctggcggcc atcgccgacc aggccatggg catgtcctgc
     4201 gccgcacggc tcaaggcccg gggcgtagcc atcaccgccc tctggaccag ttccctgacg
     4261 gtcgactacc tggacgtggc ccgacccggt cagtggctgg ccttcgacac cgggttctgc
     4321 cggccggggc gcacgatctg ccatgccgaa gccgacatta ccgccgacgg cgccaccgtg
     4381 gcccgggcca ggggcgcctt ccgggtgagc ctcgaccgga ccgaggccgc cggctagggc
     4441 ttcggggtga cagcggccgc tgcggccgcc gcgtcggcc
//
LOCUS       DAORNX010000238         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14553_length_4459_cov_7.848320, whole genome shotgun sequence.
ACCESSION   DAORNX010000238 DAORNX010000000
VERSION     DAORNX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14553_length_4459_cov_7.848320"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1286)
                     /locus_tag="PLO65_10765"
     CDS             complement(141..1286)
                     /locus_tag="PLO65_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRD28769.1"
                     /translation="MADFSSFAGRPVPDWYAEPQLGIFIHWGMASVPAFAPAGRSISD
                     LFVSDYDNAFVLGPYAEWYDNALRDPTSPTAAYHRERWGDAPYSAFREPFEQAAEAFD
                     ADAWADLFQAAGAKYVVFVTKHHDGFCLWPTGIDNPNRPGWSSKRDFVGELGEAVRAR
                     GMRYGLYYSGGLDWTFRPSLIRNMGEMFACVPTDEAYSAYALAQARELIDRYRPSVLW
                     NDIAWPNVEDLPGLFAHYYATVPDGVINDRWMASKALFESLRDPANCAAFNAMVKARI
                     AEVGVHGGSTSSPPHCDFRTVEYTTGDHMVGKGKWESTRGLGLAFGYNAAEATDDYMN
                     GPQLLELYREVTAGGGNLLINVGPRADATIPAVQREPLLALGAALRA"
     gene            complement(1353..2108)
                     /locus_tag="PLO65_10770"
     CDS             complement(1353..2108)
                     /locus_tag="PLO65_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRD28770.1"
                     /translation="MAAYRVPHPRRPHIERHARRQTLAARVIGFMLLWGLVGLMVFTA
                     DRAIPREHLPWKTLAIIDPLGAATRIKAARAGDDPAACRAILVQGGIEYAEVPATAGA
                     CGLSDTVRLRSGLAPLSPTDAPMTCKEALAFSIWERQVVQAVAFDELGQAVTGIDHYG
                     SYACRRQYGRTEGPISEHASANAIDVAAFRLADGTVISVARDWADPGPRGRFLHRVRD
                     GSCRIFLTTLSPDYNLEHHDHFHLDMGGWPKCG"
     gene            complement(2504..3055)
                     /locus_tag="PLO65_10775"
     CDS             complement(2504..3055)
                     /locus_tag="PLO65_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD28771.1"
                     /translation="MALVPFADIDALPPKAKDYWDKLAAKLNIFRMLANADTMMEPAL
                     RFGGAILSKQQLGADLRELVILLVAELEGGEYEWNQHVPIGLRCGCSQAQIDALAARR
                     LDDASFNAKERALLVFAAEVIEKVKASEASLAEAQKHFSPKEIVEIILTCGFYMTMAR
                     LTETTRTDIDPPAGSVVVDALQR"
     gene            complement(3056..3370)
                     /locus_tag="PLO65_10780"
     CDS             complement(3056..3370)
                     /locus_tag="PLO65_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRD28772.1"
                     /translation="MLEATSRIRHHVEGVVKADFLNGSTSFDAVAMNIVVIGESVVHL
                     PPDLKAGEPETPWRQIVASRNLVAHGYPELDPDVIWTIVATQLDDLDAVTKRMIARLE
                     GL"
     gene            complement(3363..3695)
                     /locus_tag="PLO65_10785"
     CDS             complement(3363..3695)
                     /locus_tag="PLO65_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD28773.1"
                     /translation="MTLDEALEKLRGAKPLLDRYGVARIGVFGSVARGEAGPDSDVDV
                     LVEFDPARVPGWEYFGLSDELAAVLKRPVDVVTARSLKRLMRDRVLGEVVYAQGRARR
                     PDAPSGYA"
     gene            3767..>4459
                     /locus_tag="PLO65_10790"
     CDS             3767..>4459
                     /locus_tag="PLO65_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD28774.1"
                     /translation="MVSSTDPSEPAGWRDLLTPALASRFAFLCLGIWLNAADALVTVT
                     LTPTIARDIGGFQYFGWAVAAFLLASIVAGAVSGRVSMTLGLRPATALAGGAYAIGCA
                     VSALAPDFLTFVAGRLVQGLGAGAVVALCYVAINALFPEKLWVRVYGAIAGVWGVATL
                     IGPLIGGVFAQGGVWRDAFWVFGAQGLIFVVATFLLVKKSTPSPQARARLPGAQLAVL
                     TAGVTMIAAAGAV"
BASE COUNT          668 a         1505 c         1551 g          735 t
ORIGIN      
        1 tgccgatgcc gcgcgagctg ccggtgatga tcgcgacctt gccggtcaga tcgaaaagcg
       61 tgccctcagc ggccatgacg tctctccctt aaacgcttgt ttgaagttgg cccgcaacgt
      121 ggcggcaagc cgacccagcg tcaagcgcgg agcgccgccc ccagggccag caggggctcc
      181 ctctggacag cggggatcgt cgcgtcggcg cgtgggccca cattgatcag caggttgccc
      241 ccgccggcgg tcacctcgcg gtacagctcc agcagctgcg ggccgttcat gtagtcgtcg
      301 gtcgcctcgg cggcgttgta gccgaaggcg aggccaagac cgcgcgtgga ctcccacttg
      361 cccttgccga ccatgtggtc gccggtggtg tattcgacgg tgcggaagtc gcagtgcggc
      421 ggcgaggagg tgctgccgcc gtgcaccccg acctcggcga tgcgcgcctt caccatcgcg
      481 ttgaacgcgg cgcagttggc cggatcgcga aggctctcga acagcgcctt cgacgccatc
      541 cagcggtcat tgatcacccc gtccggcacc gtcgcgtagt agtgggcgaa caggccgggc
      601 agatcctcga cattgggcca ggcgatatcg ttccacagca ccgaggggcg atagcgatcg
      661 atcagctccc gggcctgggc cagggcgtag gcgctgtagg cctcgtcggt gggcacgcag
      721 gcgaacatct cgcccatgtt gcggatcagg ctcggccgga aggtccaatc caggccgccg
      781 gagtagtaga gaccgtagcg catgccgcgg gcgcggaccg cctcccccag ctcgccgacg
      841 aagtcgcgtt tcgaagacca gccggggcgg ttcggattgt cgatcccggt cggccagagg
      901 cagaagccgt cgtggtgctt ggtgacgaac accacatatt ttgccccggc ggcctggaaa
      961 aggtctgccc aggcgtcggc gtcaaaagcc tcggcggcct gctcgaacgg ctcgcggaag
     1021 gcgctgtagg gggcgtcgcc ccaccgttcc cggtggtagg cggcggtcgg actggtcggg
     1081 tcacgcaggg cgttgtcgta ccactcggca tacgggccca gaacgaaggc gttgtcgtag
     1141 tcgctgacga acaggtcgct gatcgaacgg cctgcgggag cgaaggccgg gaccgaggcc
     1201 atgccccagt ggatgaagat cccgagttgc ggctcggcgt accagtcagg gaccggccgt
     1261 cccgcgaagc tgctgaagtc cgccatgttt ctccccacgc ggccgtgtcc ggcggcaagg
     1321 gtctcgcggc ctcccctagg gaaggcaacg cctcacccgc acttcggcca gccgcccatg
     1381 tccagatgga agtggtcgtg gtgctcaagg ttgtagtcgg ggctgagcgt ggtcaggaag
     1441 atgcggcagc tcccgtcacg cacccggtgc aggaagcggc ccctgggtcc gggatcggcc
     1501 cagtcgcggg cgacggagat caccgttccg tcggccagcc ggaaggccgc cacgtcgatg
     1561 gcgttggcgc tggcgtgctc gctgatcggc ccctcggtcc ggccatactg ccggcggcag
     1621 gcgtagctgc catagtgatc gatgccggtc acggcctggc cgagttcgtc gaacgccacc
     1681 gcctggacca cctggcgctc ccagatgctg aaggcgagcg cttccttgca ggtcatcggc
     1741 gcgtcggtcg gcgagagcgg cgccaggccc gagcgcagcc gcaccgtgtc gctcaggccg
     1801 caggcgccgg cggtcgccgg gacctcggcg tattcgatgc cgccctggac cagaatggcg
     1861 cggcaggcag ccgggtcgtc gccggccctg gccgccttga tccgggtcgc cgcgccaagc
     1921 ggatcgatga tcgccagggt cttccatggc aggtgttcgc gcgggatggc ccggtcggcc
     1981 gtgaacacca tcagcccgac caggccccac agcagcatga agccgatcac ccgcgccgcc
     2041 agggtctggc gtcgggcgtg cctctcgatg tgaggccggc gaggatgcgg aacgcgatag
     2101 gcggccatgg gtgtctatgg tgaacgaggt tcgccggtcc gtgggtcagg aaaatggcga
     2161 ggcggcccat agcacagatt ggcgcccgcc gaacgggcaa cgtccacagc gcgcggtctg
     2221 accgccttgg tcatggagag ccctgaaggc cttgatggaa tgatgcgctc cggacagcgg
     2281 gcctggtcaa ggacgacccc gacggggtcg ccgcttcgcg gccgccaaag gcggtccttg
     2341 agcaggaccg cgccggcgca atactggccg tcaagacatc agggacccgt ccgccatttg
     2401 tggcaggggg actccgcgcc ggcccggccg ccaccggcgc atccctctcc accagccttc
     2461 ggctggtccc ccctccccct actgggggag gaaggtcggt tggttaccgc tgcagcgcat
     2521 ccaccaccac gctgccggcc ggcgggtcga tatcggtgcg ggtggtctcg gtcagccggg
     2581 ccatggtcat gtagaagcca caggtcagga tgatctcgac gatctccttg gggctgaagt
     2641 gcttctgcgc ctcggcgagc gacgcctcgc tggccttgac cttctcgatc acctcggcgg
     2701 cgaagaccag cagggccctt tccttcgcat tgaagctcgc gtcgtccagc cggcgggccg
     2761 ccagggcgtc gatctgggcc tggctgcagc cgcagcgcag gccgatcggc acgtgctggt
     2821 tccactcgta ctcgccgccc tcgagctcgg cgaccagcag aatgaccagt tcgcgcaggt
     2881 cggcgcccag ctgttgcttg ctgaggatcg cgccgccgaa gcgcagggcc ggctccatca
     2941 tcgtgtcggc gttggccagc atgcggaaga tgttcagctt ggccgccagc ttgtcccaat
     3001 agtctttcgc cttgggcggc agggcgtcga tgtcggcgaa ggggacgagg gccattcaaa
     3061 gtccttccag gcgggcgatc atgcgtttgg tcacggcgtc gaggtcatcg agttgggtcg
     3121 cgacgattgt ccagatgaca tccggatcaa gctcgggata gccgtgcgcg accagatttc
     3181 ggctcgcgac gatctggcgc cagggcgtct ccggttcccc ggccttcagg tcaggcggca
     3241 gatggacgac agattcgccg atgacgacaa tgttcatcgc gacggcgtcg aagctcgtag
     3301 acccattcag gaaatcggcc ttcacgacgc cctcgacgtg gtggcgaatg cgcgaggtcg
     3361 cctcaagcat atcccgaagg cgcatccggg cgtcgcgcgc gaccttgggc atagacgact
     3421 tcgcccagga cgcggtcgcg catcaggcgc tttagcgatc tggccgtgac gacatccacg
     3481 ggccgcttga gcacagccgc cagttcgtcc gagagaccga aatattccca gcctggaacc
     3541 cgcgcgggat cgaactccac cagcacatcc acatcgctgt ccggtccggc ttcgccgcga
     3601 gccaccgagc cgaacacgcc gatccgcgca acgccgtacc ggtcgagcag cggcttcgcg
     3661 ccgcgaagtt tctcgagggc ttcgtcgagg gtcatgatgg gatcatagca cagatggcgc
     3721 ccccgcactt ggcggaacga tgatccgggt ccatcaacgg cgctctatgg tttcatcgac
     3781 agacccctcc gaacccgccg gctggcgcga cctgctgacc ccggccctgg cgtcgcggtt
     3841 cgcgtttctg tgcctgggca tctggctcaa cgccgccgac gccctggtga ccgtcaccct
     3901 gaccccgacc atcgccaggg acatcggcgg cttccagtat ttcggctggg cggtggccgc
     3961 cttcctgctg gcctcgatcg tcgccggggc ggtgtcgggg cgggtgtcga tgaccctggg
     4021 cctgcgcccg gccacggcgc tcgcgggcgg ggcctatgcg atcggttgcg cggtcagcgc
     4081 cctggccccg gacttcctca ccttcgtcgc cggccggctg gttcagggcc tcggcgccgg
     4141 ggccgtggtg gccctctgct acgtcgccat caacgccctg ttccccgaga agctgtgggt
     4201 gcgggtctat ggcgccatcg ccggagtctg gggcgtggcg accctgatcg gcccgctgat
     4261 cggcggggtg ttcgcccagg gcggggtatg gcgcgacgcc ttctgggtct tcggcgcgca
     4321 gggcctgatc ttcgtcgtcg ccaccttcct gctggtgaag aagtccaccc cgtcgccgca
     4381 ggcccgagcg cgcctgccag gcgcccagct ggccgtgctg acggcgggcg tgacgatgat
     4441 cgccgcggcg ggcgcggtc
//
LOCUS       DAORNX010000239         4455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14567_length_4455_cov_7.783182, whole genome shotgun sequence.
ACCESSION   DAORNX010000239 DAORNX010000000
VERSION     DAORNX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14567_length_4455_cov_7.783182"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLO65_10795"
     CDS             <1..469
                     /locus_tag="PLO65_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRD28775.1"
                     /translation="AVSGPIGDGWLGLRAARGELADADLAMRYRLPEPRLDLRALLLD
                     QARAAADISDGLLADAGHIARASGCGVSIDLGRLPLSAGAMAWLSGQPDSRAARLALA
                     TGGDDYQIVCALDPGRATPPQLTVVGVFGAGQGVTATFDGVAVPVGRLGWTHD"
     gene            505..945
                     /locus_tag="PLO65_10800"
     CDS             505..945
                     /locus_tag="PLO65_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28776.1"
                     /translation="MRRRHLLATLPLALAAAGAATASEPAKKEGGGQYVDLSPVALPI
                     VVNNRLVNYVFVHVRINLSASADAPKIREKEPWFRDALVRVGHRTPFTKATDYTVIDE
                     ARMKAALMREAAAIVPARNLLSISLISQTPKQRSGLPRPPAAPR"
     gene            1120..3282
                     /locus_tag="PLO65_10805"
     CDS             1120..3282
                     /locus_tag="PLO65_10805"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRD28777.1"
                     /translation="MGIDFLVLAIAAGLAAVIYGIVQTLGLLRKSAGNARMQEIAAAI
                     QEGAQAYLNRQYTTIGIVGVVVIAVLAWFFKSWEQPVGFALGAILSGLAGFVGMLISV
                     RANVRTAQAASESLGKGLNVAFTSGAITGMLVAGLALLGVAGYYYVLTRVMGLDPTSR
                     PVIDSLVTLGFGASLISIFARLGGGIFTKGADVGGDMVGKVEAGIPEDDPRNAATIAD
                     NVGDNVGDCAGMAADLFETYAVTTVATMVLAAIFFRGDAALVSAMMTLPLAICAVGIV
                     TSILGSFAVRLGKSQNIMGALYQGLIVTGLVSAAALYFLFPHFVSAPIEFTPFGGAAK
                     TYGAMELFWCGFTGLVITALIVVITEYYTGTGFRPVKSVSQASVSGHGTNVIQGLAMS
                     LEACAAPAIVIIAGIIVTYGLAGLFGIAIAVTAMLSLAGFIVALDAFGPVTDNAGGIA
                     EMAGLPPEVRVTTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTEDLKFFTAEGL
                     GKGGFFDGLGAVTFDLSNPYVVVGLLFGGLLPFLFGGMSMTAVGRAAQAVVVEVRRQF
                     RENPGIMTGDVKPEYGKAVDILTKAAIREMIVPSLLPVASPVVLFFVINAIAGKINAF
                     ASLGAMLMGVIVTGLFVAISMTSGGGAWDNAKKVIEEGFTDSDGVKHGKGSEAHKAAV
                     TGDTVGDPYKDTSGPAVNPMIKIANIVALLLLAVLAHG"
     gene            complement(3382..3876)
                     /gene="bamE"
                     /locus_tag="PLO65_10810"
     CDS             complement(3382..3876)
                     /gene="bamE"
                     /locus_tag="PLO65_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRD28778.1"
                     /translation="MFRKAALAAASAVVLMTTGACMPMTTYSGFQAIEVNPADIKAGE
                     DTKSTVMGKLGSPSARSTFDPNVWFYMSQVTDRVAFYQPRVAQRNIYAVTFDAATEQV
                     TAVNAYTLRDGKVFAYNSRETPTRGRELSILEQLLGNVGRGGMLPRDDEEGVPGSRPG
                     DRRE"
     gene            3989..>4455
                     /locus_tag="PLO65_10815"
     CDS             3989..>4455
                     /locus_tag="PLO65_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HRD28779.1"
                     /translation="MLQRLFRPSPAKLAGEALYAAAAGQARRPDFYRTGGVADTREGR
                     FELYTLHVILLVERLRGETGVAAEGRQALFDRYVQGLDDAFRELGVGDLSVAKRVKKL
                     GQAFYGRLKAYEAAVADLPDQTALLEVLGRTVLEGRPQTAGDLAAYVVAARAAL"
BASE COUNT          630 a         1568 c         1515 g          742 t
ORIGIN      
        1 ggcggtcagc ggcccgatcg gcgacggctg gctgggcctg cgggcggcgc gcggggaact
       61 cgccgacgcc gacctggcga tgcgctaccg cctgccggaa ccccgcctgg acctgagggc
      121 gctgctgctc gaccaggcgc gggccgcggc ggacatttcg gacggcctgc tggctgatgc
      181 gggccatatc gccagggcca gcggctgcgg cgtgagcatc gatctgggcc gattgccgct
      241 gtcggccggc gcgatggcgt ggttgtccgg tcagcccgat tcccgggccg cccggctggc
      301 gctggccacc ggcggcgatg actaccagat tgtctgcgcc ctggacccgg gacgggcgac
      361 tccgccgcag ctgaccgtgg tcggcgtctt cggggcgggg cagggcgtga cagcgacctt
      421 cgacggcgtc gcggtcccgg tcggacgtct cggctggacc cacgattaac cggctcttca
      481 ccacggcggc gtgacaagcg ggtcatgcgt cgccgtcatc tcctcgccac cctgcctctg
      541 gctctcgccg ccgccggcgc ggcgaccgcc agcgagccgg cgaagaagga agggggcggc
      601 cagtatgtcg acctgtcccc ggtggccctg ccgatcgtgg tcaacaaccg gctggtcaac
      661 tacgtcttcg tccacgtccg catcaacctc agcgcgtcgg ccgacgcccc gaagatccgc
      721 gagaaggaac cctggttccg ggacgccctg gtccgcgtcg gacaccgcac gccgttcacc
      781 aaggcgaccg actacacggt gatcgacgag gcccgcatga aagccgccct gatgcgcgag
      841 gccgcggcca tcgtgcccgc ccgcaatctg ctctcgatca gcctgatttc acaaacgccg
      901 aagcagcgca gcggcctgcc gcgtccgccg gccgcccccc gttagtttcc ccaaacggaa
      961 caggcggttg cttcgaccgc cggcatttgg caaagacggg cgtctttcgc aaagggggcg
     1021 ccgtctgacg gcgtctattc aaagcagatc ggggggaccg gacgtcggcc agcggccgcg
     1081 cgggaacctt cggggctata acaaggggct cttggaacta tgggcatcga ctttctcgta
     1141 ctggcgatag ccgccggtct ggccgccgtg atttacggca tcgtccagac cctcggcctg
     1201 ttgcgcaaat ccgccggcaa cgcgcggatg caggaaatcg ccgcggccat tcaggaaggc
     1261 gctcaggcct atctgaaccg gcagtacacc acgatcggca tcgtcggggt ggtggtgatc
     1321 gcggtcctgg cctggttctt caagagctgg gagcagccgg tcggcttcgc gctgggcgcg
     1381 atcctgtcgg gcctggccgg cttcgtcggc atgctgatct cggtgcgcgc caacgtccgc
     1441 acggcccagg ccgcgtcgga aagcctcggc aagggcctca acgtcgcctt cacctccggc
     1501 gccatcaccg gcatgctcgt ggccggcctc gccctgctgg gcgtggcggg ctactactac
     1561 gtgctgaccc gcgtcatggg cctggacccg accagccgcc cggtgatcga ctcgctcgtc
     1621 accctgggct tcggcgcctc gctgatctcg atcttcgccc gtctcggcgg cggcatcttc
     1681 accaagggcg ccgacgtcgg cggcgacatg gtcggcaagg ttgaagccgg catccccgag
     1741 gacgatcccc gcaacgcggc caccatcgcc gacaacgtcg gcgacaacgt cggcgactgc
     1801 gccggcatgg ccgccgacct gttcgagacc tacgcggtga ccacggtcgc caccatggtc
     1861 ctggccgcca tcttcttccg cggcgacgcg gcgctggttt cggcgatgat gaccctgccg
     1921 ctggcgatct gcgccgtcgg catcgtcacc tcgatcctcg gctcgttcgc cgtgcgcctc
     1981 ggcaagagcc agaacatcat gggcgccctg taccagggcc tgatcgtcac cggcctggtc
     2041 tcggccgccg ccctctactt cctgttcccg cacttcgtgt ccgccccgat cgagttcacg
     2101 ccgttcggcg gcgcggcgaa gacctacggc gcgatggagc tgttctggtg cggcttcacc
     2161 ggcctggtga tcaccgccct gatcgtggtc atcaccgaat actacaccgg cacgggcttc
     2221 cggccggtga agtcggtctc gcaggcctcg gtctccggcc acggcaccaa cgtgatccag
     2281 ggcctggcca tgtcgctgga ggcctgcgcg gccccggcca tcgtgatcat cgccggcatc
     2341 atcgtcacct acggcctggc cggcctgttc ggcatcgcca tcgccgtgac cgccatgctg
     2401 tcgctcgccg gcttcatcgt cgccctcgac gccttcggcc cggtcaccga caacgccggc
     2461 ggcatcgccg agatggccgg cctgcccccg gaagtgcgcg tcaccaccga cgccctggat
     2521 gccgtgggca acaccaccaa ggctgtcacc aagggctacg ccattggctc ggccggcctc
     2581 ggcgccctgg tgctgttcgc cgcctacacc gaggacctga agttcttcac ggccgagggt
     2641 ctgggcaagg gcggcttctt cgacggtctg ggcgcggtca ccttcgacct ctccaacccc
     2701 tacgtcgtgg tcggcttgct gttcggcggc ctgctgccgt tcctgttcgg cggcatgtcg
     2761 atgaccgccg tcggtcgcgc ggcccaggcc gtggtggtcg aggtccgtcg ccagttccgc
     2821 gagaaccccg gcatcatgac cggcgacgtc aagcccgagt acggcaaggc cgtggacatc
     2881 ctgaccaagg ccgcgatccg ggaaatgatc gtcccctcgc tgctgccggt cgcctcgccg
     2941 gtggtgctgt tcttcgtgat caacgccatc gccggcaaga tcaacgcctt cgccagcctc
     3001 ggcgccatgc tgatgggcgt catcgtcacc ggcctgttcg tggccatctc gatgacctcg
     3061 ggcggcggcg cctgggacaa cgccaagaag gtcatcgagg aaggcttcac cgacagcgat
     3121 ggcgtcaagc acggcaaggg ctctgaagcc cacaaggcgg ccgtgaccgg cgacaccgtc
     3181 ggcgaccctt acaaggacac ctccggtccg gcggtgaacc cgatgatcaa gatcgccaac
     3241 atcgtggcac tgctgctgct ggccgtgctg gcccacggct aggtcttccg gggagccggt
     3301 tcgccggctc cccgcgcagc cagaaaaaac gccccggaga gcgatctccg gggcgttgct
     3361 gtctgcggcc gggggcggcg gctattcgcg gcgatcgccg ggtcggctgc cgggaacgcc
     3421 ctcttcgtcg tcgcggggca gcatgccgcc gcgcccgacg ttgccgagga gctgttcaag
     3481 gatgctcagt tcgcggccgc gggtcggggt ttcccggctg ttataggcga ataccttgcc
     3541 gtcgcgcagg gtgtaggcgt tcaccgccgt cacctgctcg gtggccgcgt caaaggtcac
     3601 ggcgtagatg ttccgctggg ctacgcgtgg ctggtagaag gccacccgat cggtcacctg
     3661 gctcatgtag aaccagacgt tcggatcgaa ggtcgacctg gccgaggggg agccgagctt
     3721 gcccatcacg gtcgacttgg tgtcctcgcc cgccttgatg tcggccgggt tcacctcgat
     3781 ggcctggaag ccggaatagg tggtcatcgg catgcaggcg ccggtcgtca tcaggacgac
     3841 ggcggaggcg gcggcgagtg cggctttacg aaacatgtcg gctccgggtg cgcgctgtgg
     3901 gcgctggtct ttgacctatc gcccgcgggg gcttagttca atgcccgctt taggctcccc
     3961 tggcggcgaa atcgaggcga taccgacgat gctacagcgg ctgttccggc ccagtccggc
     4021 gaaactggcg ggcgaggcgc tctacgcggc cgcggctggc caggcgcgcc gacccgattt
     4081 ctaccgcacc ggcggggtcg ccgacacgcg cgagggccgc ttcgaactct acaccctgca
     4141 tgtcatcctg ctcgtcgagc gcctcagggg cgagaccggc gtcgccgccg aggggcggca
     4201 ggcgctgttc gatcgctatg tgcaggggct cgacgacgcc ttccgcgaac tgggcgtcgg
     4261 cgacctgtcg gtcgccaagc gggtcaagaa gcttggccag gcgttctacg ggcgactgaa
     4321 ggcctacgag gcggccgtcg ccgacctgcc ggaccagacc gcgctgctgg aggtgctggg
     4381 gcgcaccgtg ctcgaggggc ggccgcagac ggccggcgat ctggccgcct atgtcgtcgc
     4441 ggcgcgcgcc gccct
//
LOCUS       DAORNX010000240         4435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14667_length_4435_cov_7.989269, whole genome shotgun sequence.
ACCESSION   DAORNX010000240 DAORNX010000000
VERSION     DAORNX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14667_length_4435_cov_7.989269"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLO65_10820"
     CDS             complement(<1..250)
                     /locus_tag="PLO65_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28780.1"
                     /translation="MTPRRAGRLALSVIATACLASAGPALAQSAEPASVRSLSENAPP
                     PPPKPMEGVEAAEALNADINARNAEAAARDRAAAEDYAR"
     gene            424..2091
                     /gene="ettA"
                     /locus_tag="PLO65_10825"
     CDS             424..2091
                     /gene="ettA"
                     /locus_tag="PLO65_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRD28781.1"
                     /translation="MAAQYIFQMQGLTKAYPGGKKVFENIWLSFYPDAKIGVVGVNGS
                     GKSTLLKIMAGLDKEFSGEARAADGVKMGYLEQEPHLDPDKTVWENVIAWCEEKQIVD
                     RYNAIAAELGENYTDELMEEMTALQEKIDAGDLWDIDSKVEMAMDALRCPPNDSGVTK
                     LSGGEKRRVALARLLLSKPDMLLLDEPTNHLDAESVAWLQHHLEAFPGCVILVTHDRY
                     FLNQVTSWTLELDRGKGVPYEGNYSGWLEQKQKRVVQEQSESEARQRALTKELEWVRS
                     NAKARQTKSKARLAAYEKMVSEQENARGAQTHAVIQIPPGPRLGNVVLEVEGLEKDYG
                     DRCLFKDLSFRLPPNGIVGVIGPNGAGKSTLFKLITGQEAPDNGKIRLGETVKLAYVD
                     QSRDDLNPNDTVWQAISGGTDVMLVGKREINSRAYVGSFNFKGGDQQKKVGQLSGGER
                     NRVHLAKTLATGGNVILLDEPTNDLDIETLQNLEEALEEFAGCAVVISHDRWFLDRLC
                     THILAFEGDSHVEWFEGNFEMYEEDKKRRLGADSLIPHRIKFQKFTR"
     gene            complement(2081..3028)
                     /locus_tag="PLO65_10830"
     CDS             complement(2081..3028)
                     /locus_tag="PLO65_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28782.1"
                     /translation="MIEDSGVEGLRRQQRLIFRCVTGFLLDLVGRQIRRHDGDFTRAV
                     VYMAAIQASRPPSGGDTDIRPFSVRAIAQSMGLPYETTRRKIAELEAAGLARRLGDRG
                     YVVSPATFEGAAYRVDGEATWRTLRRVIIDLRALQFDFDQFAGGSGLAAARHLDPDDL
                     TDAANVLVNDFLLRVLEGGVEPHGSMLDAVIFATMLLANAELLTHDPQLAWTYAGAET
                     PPPDNLRRPATIIGIARPLGLTHETVRRHVRRHRERGWVRRVTGGYLITQDRIQAPDV
                     LQSGLLISHCFLQLMRSLRQLGVDPAAIEAVPGPGRPTA"
     gene            3192..4148
                     /locus_tag="PLO65_10835"
     CDS             3192..4148
                     /locus_tag="PLO65_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRD28783.1"
                     /translation="MPDTSPPPVVLISHEMLLPLQAALEPDYRVLRLWDYPDRLAFLE
                     GPGLEVQAIVHAGDIRLSRDLLSEMPRLGLIACVSVGYDGVDVAWCRAQGVAVTHARG
                     LNAEDVADHAVGAFLAAWRGIVAGDRIVRQGRWTPAERMRPRPGLTGRKAGIIGLGHI
                     GEAVARRLEPFGMIVSWWGPNPKDSRWPMAAGLTALAADSDALFVCCRGDAANHHLIS
                     RGVIEALGPRGCLVNVARGSVVDEDALIAALRDGRLGMAALDVFETEPTPAERWADVP
                     NTVLTPHTAGGTLESIPRMVAQTLENLRRFFHGEPLLSPVQA"
BASE COUNT          739 a         1496 c         1575 g          625 t
ORIGIN      
        1 ggcgggcgta gtcctcggcc gcggcgcggt cgcgggcggc ggcttcggcg ttgcgggcgt
       61 tgatgtcggc gttgagggcc tcggccgcct cgacgccctc catcggtttc ggtggaggcg
      121 gcggcgcatt ctcgctgagc gaccgaacgc tggcaggttc ggcggactgg gccagggccg
      181 ggccggcgct cgcgaggcag gcggtggcga tcacggacag ggccaggcgg ccggcgcgac
      241 gcggtgtcat ggcgattccc cgacggactg caatggccac atcctgccac agcctgggtc
      301 gccgaagcca ccgcggacac ctcacgcggg tttggttccc ttgcagccgt tgcctttcga
      361 ccccccggtc gctacatccc gcgccgaccc ccattccgat cagacgagag cgagcgaccc
      421 cccatggccg cgcaatacat tttccagatg cagggcctga ccaaggccta cccgggcggc
      481 aagaaggtgt tcgagaacat ctggctgtcg ttctacccgg acgccaagat cggcgtggtc
      541 ggggtcaacg gctcgggcaa gtcgaccttg ctgaagatca tggccgggct ggacaaggaa
      601 ttcagcggcg aggccagggc cgccgacggc gtgaagatgg gctacctcga gcaggagccg
      661 catctcgatc cggacaagac cgtctgggag aatgtcatcg cctggtgcga ggaaaagcag
      721 atcgtcgacc gctacaacgc gatcgccgcc gagctgggcg aaaactatac cgacgagctg
      781 atggaggaga tgaccgccct ccaggagaag atcgacgccg gcgacctgtg ggacatcgac
      841 agcaaggtcg agatggccat ggacgccctg cgctgtccgc cgaatgacag cggcgtgacc
      901 aagctgtcgg gcggcgagaa gcgccgcgtg gccctggccc ggctgctgct cagcaagccg
      961 gacatgctgc tgctcgacga accgaccaac cacctggacg ccgagtcggt ggcctggctg
     1021 cagcaccacc tggaagcctt ccccggctgc gtcatcctgg tgacccacga ccgctacttc
     1081 ctcaaccagg tgaccagctg gacgctcgag ctcgatcgcg gcaagggcgt gccctacgag
     1141 ggcaactact ccggctggct ggagcagaag cagaagcggg tggtgcagga gcagtcggag
     1201 tcggaggccc ggcaaagggc cctgaccaag gaactggaat gggtccgctc caacgccaag
     1261 gcccgccaga ccaagtccaa ggcccgtctg gccgcctatg agaagatggt ctccgagcag
     1321 gagaacgccc gcggggccca gacccacgcg gtgatccaga tcccgcccgg cccgcgcctg
     1381 ggcaacgtgg tgctcgaggt cgagggcctg gagaaggact acggcgaccg ctgcctgttc
     1441 aaggacctgt cgttccgcct gccgcccaac ggcatcgtcg gggtgatcgg ccccaatggc
     1501 gcgggcaagt cgacgctgtt caagctgatc accggccagg aggcgccgga caacggcaag
     1561 atccgcctcg gcgagacggt caagctggcc tacgtcgacc agagccgcga cgacctcaac
     1621 ccgaacgaca ccgtctggca ggcgatcagc ggcggcaccg acgtgatgct tgtcggcaag
     1681 cgcgagatca acagccgcgc ctatgtcggc agcttcaact tcaagggcgg cgaccagcag
     1741 aagaaggtcg gccagctgtc gggcggggag cgcaaccgcg tccacctggc caagaccctg
     1801 gccaccggcg gcaatgtcat cctgctcgac gaaccgacca acgacctgga catcgagacg
     1861 ctgcagaacc tcgaggaggc gctggaagag ttcgccggct gcgccgtggt catcagccac
     1921 gaccgctggt tcctcgaccg cctctgcacc cacatcctgg ccttcgaagg cgacagccat
     1981 gtcgagtggt tcgagggcaa cttcgagatg tacgaggaag acaagaagcg ccgcctcggc
     2041 gccgacagcc tgattccgca ccggatcaag ttccagaagt tcacgcggta gggcgcccgg
     2101 gaccggggac ggcctcgatc gccgcggggt cgacgccgag ctgacgcagg gaccgcatca
     2161 gctgcaggaa gcagtgcgag atcagcaggc ccgactgcag cacgtccggc gcctgtatcc
     2221 ggtcctgggt gatcaggtag ccgccggtga cccgcctgac ccagccgcgt tcgcgatgcc
     2281 ggcgaacatg gcggcgcacc gtctcgtggg tgaggcccag cgggcgggcg atgccgatga
     2341 tggtcgctgg tcgccgcagg ttgtccggcg gcggggtctc ggcgcccgcg taggtccagg
     2401 ccagctgcgg gtcatgggtc agcaactcgg cattggccag cagcatggtg gcgaagatca
     2461 ccgcgtcgag catcgaaccg tgcggttcga caccgccctc cagcacccgc agcaggaaat
     2521 cgttgaccag cacgttggcc gcatcggtca ggtcgtcggg gtcgagatgg cgggcggcgg
     2581 ccaggcccga gccgccggcg aactggtcga aatcgaactg cagcgcgcga aggtcgatga
     2641 ttacccgccg cagcgtccgc caggtcgcct cgccatccac gcgataggcc gccccttcga
     2701 acgtcgccgg cgacacgacg tagccccggt cgccgagccg gcgggccagg ccggccgctt
     2761 ccagctcggc gatcttgcga cgggtggtct cgtagggcag gcccatcgac tgggcgatgg
     2821 cgcgcacgct gaacgggcgg atatcggtgt caccgccgga tggcggccgg ctggcctgga
     2881 tcgcggccat gtagaccacc gcgcgcgtga aatccccgtc gtgccggcgg atctgccggc
     2941 cgaccaggtc gagcaggaag ccggtcacac aacggaagat tagccgctgc tgtcgccgca
     3001 ggccctcaac ccccgaatcc tcgatcattg gcgcgtcgtc cccgtcgaca ggaaatcccc
     3061 attctggcga aacacaaggc gacatatgtc ggcgggcgat cgcgcggccg ggcgacaacg
     3121 tgggcggtag tcccgcgccg gctcacgacc tcgtcggttg acgcgcggcg gggcgcgggc
     3181 agacttggcc catgcccgac acttcgcccc cgcccgtggt cctgatctcg cacgagatgt
     3241 tgctgccgct gcaggcggcg ctcgagcccg actatcgcgt cctgaggctg tgggactacc
     3301 cggatcgact ggcgtttctc gagggccccg gtcttgaggt gcaggccatt gtccacgccg
     3361 gcgacatccg cctgtcgcgc gacctgctgt cggaaatgcc caggctgggg ctgatcgcct
     3421 gcgtcagtgt cggctatgac ggggtggacg tcgcctggtg tcgcgcccag ggggtggcgg
     3481 tgacccacgc gcgaggcctg aacgccgagg acgtcgccga ccacgccgtc ggcgcctttc
     3541 tcgccgcctg gcgcggcatc gtcgccggag acaggatcgt gcgacagggg cgctggaccc
     3601 cggccgagcg gatgcggccg cggccgggcc tgacgggccg caaggccggc atcatcgggc
     3661 tcggccatat cggcgaggcg gtggcccggc gactcgagcc gttcggcatg atcgtctcct
     3721 ggtgggggcc gaacccgaag gacagccgct ggccgatggc ggccgggctg accgccctgg
     3781 cggcggacag cgacgccctg ttcgtctgtt gtcgcgggga cgcggccaac catcacctga
     3841 tctcacgggg ggtgatcgag gcgctcgggc cgcgcggctg tctggtcaat gtcgcgcgcg
     3901 gttcggtggt cgacgaggac gccctgatcg ccgccctgcg ggacgggcgg ctgggcatgg
     3961 cggcgttgga tgtcttcgag accgagccga cgccggccga acgctgggcc gacgtgccca
     4021 acaccgtcct gactccgcac acggccggcg gaacgctcga gagcattccc cggatggtcg
     4081 cccagaccct ggagaacctg cggcgcttct tccacgggga gccgttgctg tcgccggttc
     4141 aggcctgaga cagccacacc gccaggccgg cgaccagcag gccggcggcc aggacgagcg
     4201 cgacccggcg tcggcgcggg gacttgatga acagaccgag catggtcaga cctcccgtcg
     4261 gcgccatggc gcgccgtcgg cgccgacagt atcaaagtga tatcactttg accagagccc
     4321 tggcgcgtcc ggtccgctac aggtaggcca tggcctggga gtcgtcgtcc ccgtccggag
     4381 gcgattgcgg ggcgggggcg gcgacacgcg cgcggcccgg catggccagg gcggc
//
LOCUS       DAORNX010000241         4426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14712_length_4426_cov_5.239533, whole genome shotgun sequence.
ACCESSION   DAORNX010000241 DAORNX010000000
VERSION     DAORNX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14712_length_4426_cov_5.239533"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..349
                     /locus_tag="PLO65_10840"
     CDS             83..349
                     /locus_tag="PLO65_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28784.1"
                     /translation="MQADRRDDQDRWTSINQRQAVLDARIDAGVRDGSLTRAEAARLR
                     ADFNDLARMEADYRRDGLTNAERADLDRRFDLLSARIRADRRDR"
     gene            complement(363..638)
                     /locus_tag="PLO65_10845"
     CDS             complement(363..638)
                     /locus_tag="PLO65_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HRD28785.1"
                     /translation="MNISKPQQRTLHALAQGGAITLVRDDRGGLVAAECFNRDGWLLS
                     DCDLAVFKALKRRRFIASRNGGPYRITREGLLNLRAQVDNRVAGRAW"
     gene            897..1667
                     /locus_tag="PLO65_10850"
     CDS             897..1667
                     /locus_tag="PLO65_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD28786.1"
                     /translation="MNRYTVRQVARMSGVSVRALHHYDQIGLLRPASVGENGYRYYGR
                     EELLRLQQILFHRELEFPLEAIRAVLAAPDFDRLEALRRHRTRLAAEARRYRRLLRTL
                     DETLAALEGDATMDDKTLYQGFAPEKQADHEAWLVDRYGDRAQAEIDHGKTVMKGWKA
                     EDFAGFAAEVEAIEAALADAMADGLPPDSAAVIAIVRRHHAWVARSWKAPPTRDAYLG
                     LGQMYGDHPDFRSRYEDRAAGLLEYLQAAMTAFADAHL"
     gene            1826..1984
                     /locus_tag="PLO65_10855"
     CDS             1826..1984
                     /locus_tag="PLO65_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28787.1"
                     /translation="MAKATSVRVPEFIPAEPDLLSPAVKQVLRVLLAVGSTIMLVGFV
                     VATFVAVF"
     gene            complement(2078..3916)
                     /gene="mutL"
                     /locus_tag="PLO65_10860"
     CDS             complement(2078..3916)
                     /gene="mutL"
                     /locus_tag="PLO65_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRD28788.1"
                     /translation="MPIRRLPPETVNRIAAGEVVERPASAVKELVENALDAGASRVEV
                     EADGGGLTRILVCDDGSGLSPDELSLAVERHATSKLCPDADGTWDLLHISTLGFRGEA
                     LPSIGSVARLSISSRARGAADAHAILVEGGATGEVGPAAFAGDHGARVEVRDLFYATP
                     ARLKFMKSARAEQMAIAEELKRQAMAHEAVGFTLDLDGRRALRLTAEHPGPDGRLARL
                     SAVLGREFQDNALAIDQEREGIRLTGFAGLPTYSRGNAAHQYLFVNGRPVRDRLLQGA
                     LRGAYADYLARDRHPTAALYIELDPTFVDVNVHPAKAEVRFRDPAMVRGLIIGALRHA
                     LAGAGHRASTTVASAALAGFRPQGYQPGPSPAGFSAWQQGGWTPRAPAPVLPGMSEAT
                     ARVESVDAWSEPTAPSHGQPAAVIDPVDYPLGAARAQVHETYVVAQTRDGMVIVDQHA
                     AHERLVYERMKREMESGGVVRQTLLLPEVVDLDPAEADRVMARADELAALGLVIEAFG
                     PGAVLVRETPALLGDTDAAGLIRDIADDLAENGQALALKERLQEVCSTMACHGSVRAG
                     RRLTAAEMNALLREMEATPHSGQCNHGRPTYVELKLADIERLFGRR"
     assembly_gap    4060..4082
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4099..>4426)
                     /locus_tag="PLO65_10865"
     CDS             complement(4099..>4426)
                     /locus_tag="PLO65_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HRD28789.1"
                     /translation="GQAVAAQRTDGERLLVARAGAEKADPLIRYVVDDEFGDLAHKDK
                     SFADRVMFWRKDAPTGQAVASSDGSAPPVVVDAAAEEARLRAITGGKTVIISREKASW
                     VKLPGL"
BASE COUNT          663 a         1548 c         1570 g          622 t
ORIGIN      
        1 ggaggccgac tatcgccgcg acggcctgac caacgccgaa cgcgccgatc tcgaccgccg
       61 cttcgaccag ctcagcgccc ggatccaggc agaccgtcgt gacgaccagg accgctggac
      121 cagcatcaac cagcgccagg ccgtcctcga cgcccgcatc gacgccggcg tccgcgacgg
      181 cagcctgacc cgcgctgagg ccgcccggct gcgcgccgat ttcaacgacc tggcccggat
      241 ggaggccgac taccgccgcg acggcctgac caacgccgag cgcgccgacc tcgaccgccg
      301 cttcgacctg ctcagcgccc gcatccgggc cgaccgtcgc gaccggtagt ccggaggcgt
      361 cgtcaccagg cgcgaccggc gacccggttg tcgacctggg cccggagatt cagcagcccc
      421 tcccgcgtaa tgcggtaggg gccgccgttc cggctggcga tgaagcggcg cctcttcagg
      481 gccttgaaga ccgcgaggtc gcagtcgctc agcagccagc catcgcggtt gaagcattcg
      541 gcggcgacaa ggccgccacg gtcgtcgcgc acaagggtga tcgcgccgcc ctgggcaagc
      601 gcgtgcagcg tgcgctgctg cggtttggaa atgttcacct ggaagtccgt gtcgaggcat
      661 gtgacgtcgc gctccggcgg tggccgaagc gggcggatca gcgggcgttc gacggaccgg
      721 ttccggccgt caggcccgcg cctcgatggc aaggggtgac atggtcgcct caggttgaac
      781 ggtatgaggc tatcggcaga gacccggact ttcaagacgg ggacatgttc cccatcaacg
      841 cccttgaccc tcacgtcccg tgagacctca tacccccgtc catcggcgag gacggcatga
      901 acaggtacac ggtcaggcag gtggcgagga tgtcgggcgt ctcggtccgc gcgttgcacc
      961 actacgacca gatcggcctg ctccggccgg ccagcgtcgg ggagaacggc taccgctact
     1021 atggccgcga ggagttgctg cgcctgcagc agatcctgtt ccaccgggaa ctcgaatttc
     1081 cgctggaggc cattcgggcg gtgctggcgg cgcccgactt tgaccggctg gaggccctgc
     1141 gccgccaccg gacacggctc gcggcggaag cccggcgcta tcgccgactc ctgcgcacgc
     1201 tggatgaaac cctcgccgcc ctcgaaggag acgcgacaat ggacgacaag acactctacc
     1261 agggcttcgc gcccgagaag caggccgacc acgaggcctg gctggtcgat cgctacggcg
     1321 accgggccca ggccgagatc gatcacggca agacggtgat gaagggctgg aaggccgagg
     1381 atttcgccgg cttcgcggcc gaggtcgaag ccatcgaggc cgccctggcg gacgccatgg
     1441 cggacggcct gccgccggac agcgcggcgg tgatcgcgat cgtgcgtcgt catcatgcct
     1501 gggtggcgcg cagctggaag gccccgccga cccgtgacgc ctacctcggc ctggggcaga
     1561 tgtacggcga ccatcccgac ttccggtcgc gctacgagga tcgggcggcc gggcttcttg
     1621 agtatctgca ggccgcgatg acggccttcg cggacgcgca cctctagcct ggagggaggc
     1681 gtgagccggg cgcggcgcac gcctcccgtc cttcagaatt gacggcccgg gcgattgact
     1741 tggatcaagc gggcgaggac gatggcccta cagagggtgt ggtttaacgc gccccgcgag
     1801 gaaacgaccc ccaaggaggc cctccatggc caaggcgacc agcgtccgcg ttcccgaatt
     1861 cattcccgcc gaacccgatc tgctgtcgcc ggcggtgaaa caggtgctgc gggtgctcct
     1921 tgccgtgggc agcaccatca tgctggtcgg tttcgtcgtg gcgaccttcg tcgcggtgtt
     1981 ctagctgaag gggacatgta ccgtcttcgg tacgtgtccc ccaatcggtt ctgctcggac
     2041 aggggacacg taccgaaggc ggtacatgtc cccaagccta ccgccgcccg aacagccgct
     2101 cgatatcggc cagcttgagt tcgacatagg tcgggcggcc gtgattgcac tggccgctgt
     2161 gcggcgtcgc ctccatctcg cgcagcaggg cgttcatctc ggcggccgtc agccggcggc
     2221 cggcgcggac cgagccgtgg caggccatgg tcgaacagac ctcctgcaga cgctccttca
     2281 gggccagggc ctggccgttc tcggccaggt cgtcggcgat gtcgcggatg agtcccgccg
     2341 cgtcggtgtc gcccagcagc gcgggcgtct cgcgcaccag cacggcgccg ggaccaaagg
     2401 cctcgatcac cagcccgagc gccgccagtt cgtcggcgcg ggccatgacc cggtcggcct
     2461 cggccgggtc gagatcgacc acctccggaa ggagcagcgt ctggcggacc acgccaccgg
     2521 actccatctc gcgcttcatc cgctcgtaga ccagccgttc gtgcgccgcg tgctggtcga
     2581 cgatgaccat gccgtcgcgg gtctgggcca cgacataggt ctcgtgcacc tgggcccggg
     2641 cggcgccgag gggatagtcg acaggatcga tgacggcggc gggctggcca tgggacggcg
     2701 cggtcggctc ggaccaggcg tccacggact cgacccgggc cgtggcctcg gacatccccg
     2761 gcaggaccgg cgccggcgcg cggggcgtcc agccgccctg ctgccacgcc gagaagccgg
     2821 ccggcgacgg gcccggctga tagccctgcg gccggaagcc cgccagggcc gccgaggcca
     2881 ccgtggtcga ggcccggtga ccggccccgg ccagggcgtg gcgcagggcg ccgatgatca
     2941 ggccgcgcac catcgccgga tcgcggaagc gcacctcggc cttggccggg tggacattga
     3001 cgtcgacgaa ggtcggatcg agctcgatgt acagcgccgc cgtcggatgg cgatcgcggg
     3061 ccaggtagtc ggcgtaggct ccgcgaaggg cgccctgcag cagccggtca cgtacgggcc
     3121 ggccgttgac gaacaggtac tggtgggcgg cgttgccgcg cgaataggtc ggcaggccag
     3181 cgaaaccggt caggcgaatc ccctcgcgct cctggtcgat ggccagggcg ttgtcctgga
     3241 actcccggcc gagcaccgcc gacagtcggg ccagacgacc gtccggaccc ggatgctcgg
     3301 cggtcagccg cagggcgcgg cggccatcga gatcgagcgt gaagccgacc gcctcatggg
     3361 ccatggcctg gcgcttcagc tcctcggcga tggccatctg ctcggcgcgg gccgacttca
     3421 tgaacttgag ccgggccggg gtggcgtaga acaggtcgcg gacctcgacc cgcgccccgt
     3481 ggtcgccggc gaaggcggcc ggaccaacct cgcccgtggc gccgccctcg acgagaatgg
     3541 cgtgggcgtc tgccgcgccg cgcgcgcggc tggagatgct caaccgcgcc accgagccga
     3601 tcgacggcag cgcctcgccg cgaaagccga gcgtcgagat gtgcagcagg tcccaggtcc
     3661 cgtcggcgtc ggggcacagc ttggaggtgg cgtggcgttc gacggccagc gacagctcgt
     3721 ccggcgacag gcccgagccg tcgtcgcaga cgaggatccg cgtcagcccg ccgccgtcgg
     3781 cctcgacctc gacccggctg gcgcccgcgt cgagggcgtt ctcgaccagc tccttgaccg
     3841 cgctggccgg ccgctcgacc acctcgccgg cggcgatgcg gttgacggtc tccggaggca
     3901 ggcgacggat gggcatggcg ccagtgtagg acgattcagc gggggaatcc ttctcccctt
     3961 gatctccgcg cccgtcatgg ctcggcttgt ccgggccacc caggcacggg gaaccacgag
     4021 aaaggggcgc gaccggcgcc gcgccccctc ttgatgatcn nnnnnnnnnn nnnnnnnnnn
     4081 nngggccatg acggatttct acaggccggg cagcttcacc cagctggcct tctcgcgcga
     4141 gatgatcacc gtcttgccgc cggtgatggc gcgcaggcgg gcctcctcgg cggcggcgtc
     4201 gacgaccacc ggcggggccg agccgtcgct cgaggcgaca gcctggccgg tcggggcgtc
     4261 cttgcgccag aacatcacgc gatcggcaaa ggacttgtcc ttgtgcgcca ggtcgccgaa
     4321 ttcgtcatcg acgacgtagc ggatcaacgg atcggccttc tcggcgccgg cgcgggcgac
     4381 gagcaggcgc tcgccgtcgg tgcgctgcgc ggcgacggcc tggccg
//
LOCUS       DAORNX010000242         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14744_length_4419_cov_9.281622, whole genome shotgun sequence.
ACCESSION   DAORNX010000242 DAORNX010000000
VERSION     DAORNX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14744_length_4419_cov_9.281622"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLO65_10870"
     CDS             complement(<1..716)
                     /locus_tag="PLO65_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRD28790.1"
                     /translation="MSFLTRRIAIDREVGHVEGHGLKPTLGWPHLVALGVGAIVGTGI
                     YTLTGEAAGLAGPGAMLSFLIAGAVCAAAALCYAEMATLMPRAGSAYTYSYAVMGETV
                     AWIVGWSLILEYTVVCAAVSVGWAGYAGALILSWWPDAPQALLKGPEAGGVVNLPAVV
                     IALLVAGLLALGTRESARVNFVLVIVKLVALAGFIVLALPAFNVAHFTPFMPQGFWAH
                     EVDGVKMGVMGAASIIFFAF"
     gene            841..2646
                     /locus_tag="PLO65_10875"
     CDS             841..2646
                     /locus_tag="PLO65_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HRD28791.1"
                     /translation="MLRGTILTAALAATLGLSGVQARADETATVAVEVPTHPGRSPLD
                     ALVSDYEAWNAGQDPISAGANGDAGARSQLPDFSLAADQKRKASLVAFQARLGAIPAT
                     GLSADDALNRAFLERLIGNQIAAIDFDTGRLAFSSDNSLDSYFGYLASSSPMTSRADA
                     ENWLKRLAGAGTYYDDQIANSRRGVKTGWVQPRPIAEIALTQARKAAAAPAEGDQAVT
                     VLDQLPASIPAAEREGYRARAIALVKTVLRPAQQRYVTFMETEYVPASRSTLGAASMP
                     GGKAYYAWLVRRYTTTDLTPDEVHQIGLKEVARIRAEMDAVRAEAGFKGTHEEFLTFL
                     RTDPQFYVATREELLEKASTIAKRIDDQLPRFFATLPRLTYGVREVPREIEVGYTTGR
                     YFPGSPANGVAGGYMVNTSALDQRGLYELPALTLHEAVPGHHLQIALGQELEGVPAFR
                     RDADMTAFTEGWGLYSEKLGLEMGIYRTPYEKFGRLSYEMWRACRLVADTGIHWMGWS
                     REQARKCFTENSALSPLNIEIELDRYISWPGQATAYKIGEMKILELRHRAEAALGERF
                     NIRRFHDAVLLAGPLPMDLLEVRIDQWIAAEKAKG"
     gene            complement(2727..2966)
                     /locus_tag="PLO65_10880"
     CDS             complement(2727..2966)
                     /locus_tag="PLO65_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HRD28792.1"
                     /translation="MKLFTDHPHSVGETYGEHLRVASSFGFAMIGAGLASVLHGLCPW
                     LFQSTGSRTIKRLYHRLTGRGPAEAGYGDWEGAGV"
     gene            complement(3123..3755)
                     /locus_tag="PLO65_10885"
     CDS             complement(3123..3755)
                     /locus_tag="PLO65_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD28793.1"
                     /translation="MSEVQSLQRDTPKSRRTRARILDEAVRVIAGQGYAATTNAAVAE
                     AAGITRGAMLYHFPTRESLLEAAIDHIQTQRAALFRDAADSLPPGADVTEHAIETYWD
                     LLHRTPFVAFAELEAASRTDPLVREMLSPSMAEFDRAQIGDHFLKMLHAGAGPRFQAS
                     RDLARFMLEGMARAQVTYDREERIERLLAVIKRATHMLNRKGGVTDLWPE"
     gene            3870..>4419
                     /locus_tag="PLO65_10890"
     CDS             3870..>4419
                     /locus_tag="PLO65_10890"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRD28794.1"
                     /translation="MSQGEPNFSCRSVFISDVHLGTRGCQAEMLLDFIRHLECDHLYL
                     VGDIIDGWKLKGGWHWPQSHNDVVQKVLRMARKGVQVTYVPGNHDERARDFCGVHFGG
                     VVVARDAIHETADGRRFLVVHGDEFDGIVQHARWLAFLGDWSYRTLLLLNTLVARARR
                     RMGLGYWSFSAYLKVKVKNALQF"
BASE COUNT          714 a         1509 c         1538 g          658 t
ORIGIN      
        1 tagaaggcga agaagatgat cgaggcggcg cccatcaccc ccatcttcac gccgtcgacc
       61 tcgtgggccc agaagccctg cggcatgaac ggcgtgaagt gggcgacatt gaacgcgggc
      121 agggccagga cgatgaagcc ggccagggcg accagcttga cgatgaccag cacgaagttg
      181 acccgggcgc tctcgcgcgt gccgagggcc agcaggccgg ccaccagcag ggcgatgacc
      241 accgccggca aattgacgac gccgccggcc tcggggccct tgagcagagc ctggggcgcg
      301 tccggccacc agctcaggat cagcgctccg gcgtagccgg cccagccgac cgaaacggcc
      361 gcgcagacca cggtgtattc gaggatcagg ctccagccga cgatccaggc gacggtctcg
      421 cccatcaccg cgtagctgta ggtgtaggcg ctgccggcgc ggggcatcag ggtcgccatc
      481 tcggcgtagc acagggccgc cgccgcgcag accgccccgg cgatcaggaa gctgagcatc
      541 gcgccgggtc cggccaggcc cgcggcctcg ccggtcaggg tgtagatgcc ggtgccgacg
      601 atggccccga cgccgagcgc caccagatgc ggccagccga gcgtcggctt tagcccgtgg
      661 ccttcgacat ggccgacttc acgatcgatg gcgatgcgcc gcgtcaggaa gctcatgagc
      721 ccctcccccc gagtcaaggc gacgaccatg gcccgctccg ggcccgttcg ccaagccccc
      781 ggcttgcggg cgcgctcgcg gggcgccagc ttgtcggccg ccgatgaggg gatgagtgcg
      841 atgcttcgtg gaacgatact gacggcggcc ctggccgcga cgctgggcct gtccggcgtc
      901 caggcgcggg cggatgagac cgcgactgtc gccgtggagg ttccgaccca tcccggccgg
      961 tcgccgctgg acgccctggt cagcgactac gaggcctgga acgccgggca ggatccgatc
     1021 tccgccggcg ccaacggcga cgccggagcg cgcagccagt tgccggactt ctccctggcc
     1081 gcggaccaga agcgcaaggc gtccctggtc gccttccagg cccggctggg ggccatcccg
     1141 gcgacgggcc tgtccgccga cgacgccctc aaccgcgcct ttctcgaacg gctgatcggc
     1201 aaccagatcg cggccatcga tttcgacacc ggccgcctgg ccttctcgag cgacaattcc
     1261 ctcgactcct atttcggtta tctggccagc agttcgccga tgacctcgcg ggccgacgcc
     1321 gaaaactggc tcaagcggct ggcgggggcc gggacctact acgacgacca gatcgccaac
     1381 tcccggcgcg gggtgaagac cggctgggtg caaccccgcc cgatcgccga gatcgccctg
     1441 acccaggccc gcaaggccgc ggcggccccg gccgagggcg atcaggccgt gaccgtgctc
     1501 gaccagctgc cggcctcgat cccggcggcg gagcgggagg gctaccgcgc ccgcgccatc
     1561 gccctggtga agaccgtcct gcgcccggcc cagcagcgct acgtgacctt catggagacg
     1621 gaatacgttc cggccagccg ctccaccctg ggcgccgcca gcatgcccgg gggcaaggcc
     1681 tactacgcct ggctggtgcg gcgctacacg accaccgacc tgaccccgga cgaggtccac
     1741 cagatcggcc tgaaggaagt cgcccgcatc cgcgcggaga tggacgccgt ccgcgccgag
     1801 gccgggttca agggcacgca cgaggaattc ctgaccttcc tgcgcaccga cccgcagttc
     1861 tacgtcgcca cccgggagga actgctggag aaggccagca ccatcgccaa gcggatcgac
     1921 gaccagttgc cgcgcttctt cgccaccctg ccccggttga cctacggcgt acgcgaggtg
     1981 ccgcgcgaga tcgaggtcgg ctacacgacc ggccgctatt tcccgggcag tccggccaac
     2041 ggcgtcgccg gcggttacat ggtcaacacc tcggccctcg accagcgcgg tctttacgag
     2101 ctgccggcgc tgacgctgca cgaggccgtg cccgggcacc atctgcagat cgccctgggc
     2161 caggagctgg agggggtgcc ggccttccgc cgcgacgccg acatgaccgc cttcaccgag
     2221 ggatggggcc tctattcgga gaagctgggc ctggagatgg gcatctaccg gacgccctac
     2281 gagaagttcg gccggctcag ctacgagatg tggcgcgcct gccggctggt cgccgacacc
     2341 ggcatccact ggatgggctg gtcgcgggaa caggcccgca agtgcttcac cgagaacagc
     2401 gccctgtcgc cgctgaacat cgagatcgaa ctggaccgct acatctcctg gccgggccag
     2461 gccacggcct acaagatcgg cgagatgaag atcctcgagc tgcgccacag ggcggaggcg
     2521 gcgctgggcg agcggttcaa tatccgccgg ttccacgacg cggtgctgct ggccgggccg
     2581 ctgccgatgg acctgctgga agtccgcatc gaccagtgga tcgcggcgga gaaggccaag
     2641 gggtagggac atgcacttca gtgcgtgtcc cttgcagctt caagcggctg gggacaccta
     2701 cctgcggtac atgtccccga tcggctctag acccccgccc cttcccagtc gccgtagccg
     2761 gcctcggccg ggccgcggcc ggtcaggcgg tggtacagcc gcttgatcgt gcggctgccg
     2821 gtggactgga acagccaggg gcagaggccg tggagcaccg aggcgaggcc cgcgccgatc
     2881 atcgcgaagc cgaagctgga ggcgaccctg aggtgttccc cgtaggtctc gccgacggaa
     2941 tggggatggt cggtgaacag tttcactgcg gtggcccttt ccgctgaact gaccgaggtg
     3001 tcgtccgtgt cgcgggggcg ggtcaagccg gggacatgta ccgaaggtac gtgtccccta
     3061 atcctgatca caggcgcggc tcgtttaggg gacacgtacc tgaaggtaca tgtccccgac
     3121 gtttactcgg gccagaggtc ggtcacgccg cccttgcggt tgagcatgtg ggtggcgcgc
     3181 ttgatcaccg ccagcagccg ctcgatgcgc tcctcccggt cgtaggtcac ctgggcccgg
     3241 gccatgccct cgagcatgaa gcgggcgagg tcgcggctgg cctggaagcg cgggccggcg
     3301 ccggcgtgca gcatcttgag aaagtggtcg ccgatctggg cccggtcgaa ctcggccatc
     3361 gaggggctga gcatctcgcg gaccagcggg tcggtgcgtg acgccgcctc cagttcggcg
     3421 aaggccacga acggcgtgcg gtgcagcagg tcccagtagg tctcgatcgc atgctcggtc
     3481 acgtcggcgc cgggcggcag gctgtcggcg gcgtcgcgga acagggccgc gcgctgggtc
     3541 tggatatgat cgatcgccgc ctcgagcagg ctttcgcggg tcgggaaatg gtagagcatc
     3601 gccccccgcg tgatgcccgc cgcctcggcc acggcggcgt tggtggtggc cgcgtagccc
     3661 tgcccggcga tgaccctgac cgcctcgtcg aggatacgcg cgcgggtccg tcgggacttg
     3721 ggggtatcac gctgcaggga ctggacctcg gacatgccgc tcatctaggg gcggcccggt
     3781 caggccgcaa cggacgagat gtcgcttcac gagactgaca cgcaatcagc ctagcagcgt
     3841 cccagtcacc gcctgtttca ggagcaggga tgagccaggg cgagccgaac ttcagctgcc
     3901 ggagcgtgtt catcagcgac gtccatctcg gcacccgtgg atgccaggcg gagatgctgc
     3961 tggacttcat ccgccacctg gaatgcgacc atctctacct ggtcggcgac atcatcgacg
     4021 gctggaagct gaagggcggc tggcactggc cgcaaagcca caacgacgtg gtgcagaagg
     4081 ttctgcggat ggcccggaag ggggtccagg tgacctatgt gccgggcaat cacgacgagc
     4141 gggcccgtga cttctgcggc gtgcatttcg gcggggtggt ggtggcccgc gatgcgatcc
     4201 acgagaccgc cgacggccgc cgattcctgg tcgtgcatgg cgacgagttc gacggcatcg
     4261 tgcaacacgc ccgctggctc gccttcctcg gcgattggtc ctatagaacc ctccttctgc
     4321 tgaacacatt ggtcgcccgc gcacggcgac ggatgggttt gggttactgg agcttttcgg
     4381 cctatctgaa ggtcaaggtg aagaacgccc tgcagttca
//
LOCUS       DAORNX010000243         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14757_length_4417_cov_8.111188, whole genome shotgun sequence.
ACCESSION   DAORNX010000243 DAORNX010000000
VERSION     DAORNX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14757_length_4417_cov_8.111188"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="argS"
                     /locus_tag="PLO65_10895"
     CDS             <1..1671
                     /gene="argS"
                     /locus_tag="PLO65_10895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRD28795.1"
                     /translation="IAAAVVEALSGHPLLQSVEIAGLGFINLRVSARALSDRAREIAA
                     DPRTGAGEVAEPRRVMVDYGGPNVAKPMHVGHLRASIIGESVKRLYRFRGDEVLGDAH
                     FGDWGFQMGLLIVAACDEDSFVRTLMERLAQTPRDFSAADEKQVMDALGEHIDLPMLD
                     RLYPEAAGRAKTDVDYRDRARRATAELQGGRFGYRLLWKHFVRVSRVALEREFHALGI
                     DFDLWKGESDVDSLIEPMVRELETKGLLADDQGARVIHLARPGETRKKKRPDGTVYEA
                     PSPDPLLVVSSEGSAMYGTTDLATVLDRRRSFDPHLILYCVDQRQADHFEQVFRAACL
                     AGYASEGSLEHIGFGTMNGPDGKPFKTREGGVLKLHDLIEMTRAKARERLHEAGLGSE
                     LDPAEFEQTAHKVAVAALKFADLSNFRGTSYIFDLDRFSSFEGKTGPYLLYQAVRIKS
                     LLRRAEGDRLRPGPITVAEPAERELAMLLDAFDAALAEAYDKKAPNFVAEHAFKLAQS
                     FSRFYAACPILQAGDDAKAASRLALAVTTLKQLELALDLLGIETPERM"
     gene            1716..1940
                     /locus_tag="PLO65_10900"
     CDS             1716..1940
                     /locus_tag="PLO65_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRD28796.1"
                     /translation="MSERYQVHAHWDAEAEVWVSTSNIPGLHVEADTLGEFMELVETL
                     APEMLADNLGIKGPVPVEVLANRTLDLVVA"
     gene            2005..2199
                     /locus_tag="PLO65_10905"
     CDS             2005..2199
                     /locus_tag="PLO65_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRD28797.1"
                     /translation="MPHDFYPDLVRILKGAGWHRYSGGKGSHEKWRHPASGKTLVVPR
                     TKSRHTANDILKDAGLPKAF"
     gene            2237..>4417
                     /locus_tag="PLO65_10910"
     CDS             2237..>4417
                     /locus_tag="PLO65_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRD28798.1"
                     /translation="MSGLRVFGIVLALCGVMVGPMAAASGGDRPIAKAVEGLVRQDGL
                     IPVFVDTRAGKIMLRLPPPDADGVQGRYLYQTYLRAGLGSNPVGLDRSAPGDTQVLVF
                     RRAGGKVLVQFENYSFVGEHGSADERQAVRESFAISTVWSTDVAAQDADGAVLIDVSG
                     FLTRDAMGVARQLKTSDQGEFKLAADLSYADTGAVAAFPRNIELEAVQTFTSDAPGPE
                     VRGILPDPRNVTLTLHHSLVALPEPGFEPRFSDPRVGAIDHPVSDFTTPLGQPMVLQL
                     AHRFRLEKTDPTAARSPVKQPIVFYIDRSAPEPVRSALMDGAKWWAQAFDAAGFIDAF
                     KVEVLPAGVSPLDARYNVVNWVHRETRGWSYGHSVVDPRTGEIVKGDVLLGSQRVRQD
                     RIIFEGLVGADKTGQGGPNDPIQVALARLRQLATHEIGHAIGLQHNFAASTYGRASVM
                     DYPAPLITIRDGQLDLSDAYATGAGAWDKFAVTWLYGTVAPGQAGRAELSSMVDRALA
                     GGLRFVSDDDARSPDTAQPLGAVWDNGADPVAELRHLMEVRRVALSRFGLASLPVGEP
                     VAELRRRIVPVYLFHRYQIDATVKLVGGVDFTYGARGDGHEASSPVPADRQRQAIDAL
                     LAAVNPAELDLGDGLLDLLTASQATTVAKQTSVELFQEGPVFDLAAAADVVAGQVFGD
                     LFAPARLNRLVEARRRDPSQPGVEELLARSVAAVSMPASGRQAEL"
BASE COUNT          674 a         1591 c         1494 g          658 t
ORIGIN      
        1 atcgccgccg cggtggtcga ggccctgtcg ggtcatcccc tgctgcagtc ggtagagatc
       61 gccggcctcg gcttcatcaa cctgcgggtc agcgcccggg cgctgtccga ccgcgcccgc
      121 gaaatcgccg ccgacccgcg caccggcgcc ggcgaggtcg ccgagccacg ccgcgtcatg
      181 gtcgactacg gcggccccaa cgtggccaag ccgatgcatg tcggccacct gcgcgcctcg
      241 atcatcggcg aatccgtcaa gcggctctat cgcttccgcg gcgacgaggt tctgggcgac
      301 gcccacttcg gcgactgggg cttccagatg ggcctgctga tcgtggcggc ctgcgacgag
      361 gacagcttcg tccgcaccct gatggagcgc ctggcgcaga ccccgcgcga cttcagcgcc
      421 gccgacgaga agcaggtgat ggacgccctg ggcgaacaca tcgacctgcc gatgctcgac
      481 cgcctgtatc cggaggccgc cggccgggcc aagaccgacg ttgactatcg cgacagggcc
      541 cgccgcgcga ccgccgagct gcagggaggg cgcttcggct atcgtctgct gtggaagcat
      601 ttcgtccggg tcagccgtgt ggcgctggag cgcgagttcc acgccctggg catcgatttc
      661 gacctgtgga agggcgagag cgacgttgac agcctgatcg agccgatggt ccgcgagctg
      721 gagaccaagg gcctgctggc cgacgaccag ggcgcccggg tgatccatct ggcccggccc
      781 ggcgagaccc ggaagaagaa gcggccggac ggcacggtct acgaggcgcc gagccccgat
      841 ccgctgctgg tggtctcctc ggaaggctcg gcgatgtacg gcacgaccga cctggccacc
      901 gtgctggacc ggcgccggtc gttcgacccg cacctgatcc tctactgcgt cgaccagcgc
      961 caggccgatc acttcgagca ggtgttccgc gccgcctgcc tcgccggcta cgcgagcgaa
     1021 gggtcgctcg agcacatcgg cttcggcacc atgaacgggc ccgacggcaa gccgttcaag
     1081 acccgcgagg gcggagtcct gaagcttcac gacctgatcg agatgacccg cgccaaggcc
     1141 cgcgaacggc tgcacgaggc gggcctcggc agcgagcttg atccggccga gttcgagcag
     1201 acagcgcaca aggtggccgt ggccgccctg aagttcgccg acctgtcgaa cttccgcggc
     1261 accagctaca tctttgacct cgaccgcttc tccagcttcg agggcaagac cggcccctac
     1321 ctgctgtacc aggcggtgcg catcaaatcg ctgctgcggc gggccgaggg cgaccgcctg
     1381 cggcccggcc ccatcacggt cgccgagccc gccgagcgcg aactggccat gctgctggac
     1441 gcgttcgatg ccgccctggc cgaggcctac gacaagaagg cccccaactt cgtcgccgaa
     1501 cacgccttca agctggccca gagcttctcg cgcttctacg ccgcctgccc gatcctgcag
     1561 gccggcgacg acgccaaggc ggcctcgcgc ctggccctgg ccgtcaccac gctgaagcag
     1621 ctcgagctgg cgctggacct gctgggcatc gagacgccgg agcggatgta ggcggcggac
     1681 tagccgtttt ccgcgcagag gcgcatgatg cagccatgag cgagcgttat caggttcacg
     1741 cacactggga tgccgaggcc gaggtctggg tatcgacgag caatattccc ggactgcatg
     1801 tcgaggccga cacactgggc gagttcatgg agctggttga gacgctggct ccggagatgc
     1861 tggccgacaa cctcggcatc aaaggtccag tgcccgtcga ggtgctggcg aaccggacgt
     1921 tggacctcgt cgtagcctga atttatcgaa cgcccgggtc ggttgtgttt aaatggaagc
     1981 acaactgatc ctcggttcgc cccgatgcca cacgacttct atcccgacct tgtgcgcatc
     2041 ctgaagggag ccggctggca ccgctactcc ggcggcaagg gcagccatga gaagtggcgc
     2101 caccccgcat caggaaagac gctcgtcgtt ccacgcacca agagccgcca caccgcgaac
     2161 gacattctga aggatgcggg cctccccaag gccttctgat cgcgccatag tcgccccctg
     2221 cctgggggag ttcagcgtgt cgggattgcg ggttttcggg attgttctcg ccctgtgcgg
     2281 cgtcatggtc gggccgatgg ccgcggcgag cgggggcgac aggccgatcg ccaaggcggt
     2341 tgagggcctg gtccgccagg acggcctgat ccccgtgttc gtcgacacca gggccggcaa
     2401 gatcatgctg cgcctgccgc cgcccgacgc cgacggagtg cagggccgtt acctctacca
     2461 gacctatctt cgcgccggcc tcggctcgaa cccggtcggc ctcgaccgct cggcgcccgg
     2521 cgacacccag gtgctggtgt tccgccgggc gggcggaaag gtcctcgtcc agttcgagaa
     2581 ctacagcttc gtcggcgagc atggctcggc cgacgagcgc caggcggtcc gcgagtcctt
     2641 cgccatctcg accgtctggt cgaccgacgt ggcggcgcag gacgctgacg gcgcggtgct
     2701 gatcgatgtc tccggctttc tgacgcgcga cgccatgggc gtcgcccgcc agctcaagac
     2761 cagcgaccag ggcgagttca agctggccgc cgacctcagc tacgccgaca ccggggcggt
     2821 cgccgccttc ccgcgcaaca tcgagctgga ggccgtccag accttcacca gcgatgcgcc
     2881 ggggccggag gtgcgcggca tcctgcccga cccgcgcaat gtcaccctga ccctgcacca
     2941 ttcgctggtc gccctgcccg agccggggtt cgagccccgc ttcagcgatc cgcgcgtcgg
     3001 ggccatcgac catccggtca gcgacttcac caccccgctc ggccagccga tggtgctgca
     3061 gctcgcgcac cgcttccggc tggagaagac cgacccgacg gccgcccgct cgccggtcaa
     3121 acagccgatc gtgttctaca tcgaccgcag cgcgcccgag ccggtccgct cggccctgat
     3181 ggacggggcc aaatggtggg cccaggcgtt cgacgccgcc ggcttcatcg acgccttcaa
     3241 ggtcgaggtg ctgccggccg gggtcagccc gctggacgcc cgatacaatg tggtcaactg
     3301 ggtccaccgc gagacgcgcg gctggtccta cggccattcg gtggtcgacc cgcgcacggg
     3361 ggagatcgtc aagggcgatg tgctgctggg ctcgcagcga gtgcgccagg accggatcat
     3421 cttcgaaggg ctggtcggcg ccgacaagac cggccagggc ggtcccaacg atccgatcca
     3481 ggtcgccctg gcccggctgc gccagctggc gacgcacgag atcggccacg ccatcggcct
     3541 gcagcacaat ttcgcggcca gcacctatgg ccgcgcctcg gtgatggact atcccgcgcc
     3601 gctgatcacc atccgcgacg gccaactcga cctgtcggac gcctacgcca ccggggccgg
     3661 cgcctgggac aaattcgccg tcacctggct gtacggcacg gtcgcgcccg gccaggcggg
     3721 tcgggcggaa ctgtcgtcga tggtcgaccg ggccctggcc ggcggcctgc ggttcgtctc
     3781 cgacgacgac gcccgctcgc ccgacacggc ccagccgctc ggggccgtgt gggacaacgg
     3841 cgccgatccg gtggccgagc tgcggcatct gatggaggtc cggcgcgtcg ccctgtcacg
     3901 cttcggcctg gccagcctgc cggtcggcga accggtggcc gagctgcgcc ggcgcatcgt
     3961 gccggtctac ctgttccacc gctaccagat cgacgccacg gtcaagctgg tcggcggggt
     4021 ggacttcacc tacggcgccc gcggcgacgg ccacgaggcc tccagccccg ttccagccga
     4081 tcgccagcgc caggccattg acgccctgct ggccgccgtc aacccggccg agctcgacct
     4141 cggcgacggc ctgctcgacc tgctgaccgc cagccaggcg accacggtgg ccaaacagac
     4201 cagcgtcgaa ctgttccagg aagggccggt gttcgacctc gccgccgccg ccgacgtggt
     4261 cgccggccag gtgttcggcg acctgttcgc cccggcccgc ctgaaccggc tggtcgaggc
     4321 gcggcgtcgt gatccgtcgc agcccggcgt ggaggagctg ctggccagga gcgtcgccgc
     4381 ggtcagcatg ccggcgtccg gacgccaggc cgagctg
//
LOCUS       DAORNX010000244         4403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14834_length_4403_cov_6.710902, whole genome shotgun sequence.
ACCESSION   DAORNX010000244 DAORNX010000000
VERSION     DAORNX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14834_length_4403_cov_6.710902"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLO65_10915"
     CDS             <1..288
                     /locus_tag="PLO65_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28799.1"
                     /translation="NEPFWRVDIKGTDIVLTRPDAAAATATNAGLAATESRAIWTAQA
                     GATPVTVTVSAGDCSDGMSDLKYGYSAQVVWGGETLKGCAFPTAAEPREGQ"
     gene            complement(292..423)
                     /locus_tag="PLO65_10920"
     CDS             complement(292..423)
                     /locus_tag="PLO65_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28800.1"
                     /translation="MAVKAVFAAATVLAIGIAMLLPVPNNRSEAHTSSIPTMEPGRG"
     gene            complement(610..1785)
                     /locus_tag="PLO65_10925"
     CDS             complement(610..1785)
                     /locus_tag="PLO65_10925"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRD28801.1"
                     /translation="MREAVIVSYARTGLAKAGRGGFNATPPMSMAAHAIKHAVAKSGV
                     ELDFVEDCYLGNNAHGAANIGRLAALLAGMPVTTGGVTINRFCSSGLQAIAMGANYIR
                     ADGGDCVVAGGVESISIPGGGMGGSGTVDPKLLEIAPAIFMAMIDTADIVAKRYNISR
                     EYQDEYSLQSQQRMAAAQAANLFADEIVPMATQMKVVNKETKEESMVDYVVTKDECNR
                     PETTLEGLASLQPVKGPGNFVTAGNASQLSDGAAAVVIMEAKEAERRGLEPLGRFVSW
                     AAAGCEADEMGIGPVFAVPKLLARQGLKVDDIDLWELNEAFASQCLYSRDKLGIDPAK
                     YNVNGGSIAIGHPFGMTGARCTGHLLQEGRRRKAKWGVVTMCIGGGQGGAGLFEIYS"
     gene            1970..3712
                     /locus_tag="PLO65_10930"
     CDS             1970..3712
                     /locus_tag="PLO65_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD28802.1"
                     /translation="MTERAFDLVIRNGAVIDGSGVPAFTADVGVKDGLIAAIGPGLPP
                     GREEIDAAGRIVTPGFVDIHTHYDGQATWDERLQPSSWHGVTTAVMGNCGVGFAPCRP
                     EDHDRLIRLMEGVEDIPFPVLTEGLPWTWESFPQYLDAVGARRFDIDIAAQLPHAALR
                     VYVMGERGANREPATAEDIAAMATIARAAVEAGALGFSTSRTLNHRTSDGQPTPTLTA
                     GEDELTGIALGLKAAGKGVLQFVSDFQNPAEEFAMFRRLVEASGRPLSFSLAQSARGK
                     DAWKLLLAALSDAVDSGLPMKAQVAGRPVGVLFGLELTLNPFSQHETYREIAHLPLAQ
                     RVAHLSDPAFRARLLSEQLDPKGPFAGSALRAWDNMFLMTEAPEYEPTQDQTVKALAA
                     GRGLWPDEVALDHMLSNGGRGMLYHPFLNYADGSLDPSFAMLSHRDTVPGLSDGGAHV
                     GMICDGSFPTSNLTHWTRDRTRGPKLALEEVIRMQTRDTAAAVGLLDRGQIAVGLRAD
                     LNVIDYDGLTLHAPGVAYDLPAGGRRLVQRADGYVATIVAGQITYRDGEPTGALPGRL
                     VRGARSAPVAMAAE"
     gene            complement(3831..4268)
                     /locus_tag="PLO65_10935"
     CDS             complement(3831..4268)
                     /locus_tag="PLO65_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD28803.1"
                     /translation="MTGSIDPTRPQFDAFKALPRDEPIHMLNLVRVRALADYPEGHPN
                     HGKGMTGLEAYRAYGRESAHIFKRVGGRQVWAGRPDVVVTGPTDERWDIAFIAEYPGG
                     GAFLAMVTDPEYREVVKHRQAGVEDSRLIRMAPITELGEGFGE"
BASE COUNT          660 a         1471 c         1591 g          681 t
ORIGIN      
        1 aacgagccgt tctggcgggt cgacatcaag ggaaccgata tcgtgctgac gcgccccgac
       61 gccgcggccg cgacggcgac caacgcgggc ctggcggcca cggagagccg ggcgatctgg
      121 acggcgcagg ccggcgcgac cccggtcacg gtgaccgtgt cggcgggcga ctgctccgac
      181 ggcatgagcg acctgaagta tggctattcg gcccaggtgg tctggggtgg agaaaccctg
      241 aagggctgtg ccttcccgac ggcggccgag ccgcgcgaag ggcaatagcg tctagccgcg
      301 tcccggttcc atcgtcggga tcgacgaggt gtgggcttcc gagcggttgt tcggcaccgg
      361 cagaagcatg gcgatgccga tggccagaac ggtggcggcg gcgaagacgg ccttgacggc
      421 catggtcgac tctcctgtgg gcgcgacacg gacataattg cgcccacaga cttaacggcc
      481 gatgaaccaa ccggttgccg cccaatacag aacggccccc ggggaggtcc cgggggccgc
      541 tgcatcaagt agatcgaggc agagatcgaa accacgtccc gactttccgg gccgcggtcc
      601 agatcacgct cagctgtaga tttcgaacag gccggcgccg ccctggccgc cgccgatgca
      661 catcgtcacc acgccccact tggccttgcg gcgccggcct tcctgcagca ggtggccggt
      721 gcagcgcgcg ccggtcatgc cgaaggggtg gccgatggcg atcgagccgc cgttgacgtt
      781 gtacttggcc ggatcgatgc cgagcttgtc gcggctgtag aggcactggc tggcgaaggc
      841 ctcgttgagc tcccacaggt cgatgtcgtc gaccttgaga ccctggcggg ccagcagctt
      901 gggcaccgcg aacaccgggc cgatgcccat ctcgtcggct tcgcagccgg cggcggccca
      961 ggacacgaag cggccgagcg gctcgaggcc acggcgttcg gcttccttgg cttccatgat
     1021 cacgaccgcg gcggcgccgt ccgacagctg gctggcgttg cccgcggtga cgaagttgcc
     1081 cgggcccttg accggctgca gcgaggcgag gccttcaagg gtggtctccg gacggttgca
     1141 ctcgtccttg gtgaccacgt agtcgaccat ggactcctcc ttggtctcct tgttgacgac
     1201 cttcatctgg gtcgccatcg gcacgatctc gtcggcgaac aggttggcgg cctgggccgc
     1261 ggccatgcgc tgctgcgact gcagcgaata ctcgtcctgg tattcgcggc tgatgttgta
     1321 gcgcttggcc acgatgtcgg cggtgtcgat catggccatg aagatggccg gggcgatctc
     1381 cagcagcttg ggatcgaccg tgccggagcc gcccatgccg ccgccgggga tggagatgct
     1441 ctccacgccg ccggccacga cgcagtcgcc gccatccgcg cggatgtagt tggcgcccat
     1501 ggcgatggcc tgcaggcccg aggagcagaa gcggttgatc gtcacgccgc cggtggtcac
     1561 cggcatgccg gccagcaggg cggccagtcg gccgatattg gccgcgccat gggcgttgtt
     1621 gcccaggtag cagtcctcga cgaagtccag ctcgacgccg gacttggcca cggcgtgctt
     1681 gatggcgtgc gcggccatcg acatcggcgg ggtggcgttg aacccgccgc gacccgcctt
     1741 ggcgaggccg gtgcgggcgt aggacacgat aacggcttcg cgcatggcgt tttccctttg
     1801 ctctcaaaca acagtttgaa ttgtcgccgg cgaccctagg cgaagcgaat cgaaaggaaa
     1861 agttcacgcg cgcgtaaacc ggccattccg gggccgggat gtcgcatgcg gcttgacgtt
     1921 cgcgtcaccc ggcccgacga tcctgatcaa cgataaggga cggaaagaca tgaccgagcg
     1981 ggcatttgat ctggtcatcc ggaacggggc ggtgatcgac ggttccggcg tgccggcctt
     2041 cacggccgac gtcggggtca aggacgggct gatcgccgct atcgggccgg gcctgccgcc
     2101 ggggcgggag gagatcgacg ccgccggccg gatcgtgacc ccgggattcg tcgacatcca
     2161 tacccactac gatggccagg cgacctggga cgaacggctg cagccctcgt cctggcacgg
     2221 cgtgaccacg gcggtgatgg gcaactgcgg cgtcggcttc gcgccctgcc ggccggagga
     2281 ccacgaccgg ctgatccggc tgatggaggg ggtcgaggac attcccttcc cggtcctgac
     2341 cgagggcctg ccgtggacct gggagagttt cccgcagtat ctcgacgcgg tcggcgcccg
     2401 gcggttcgac atcgacatcg ccgcccagct gccgcacgcg gcgctgcggg tctatgtgat
     2461 gggcgagcgc ggggccaacc gcgagccggc gacggcggag gacatcgcgg ccatggcgac
     2521 catcgcccgg gccgccgtcg aggccggggc gctcggcttt tccacctcgc ggaccctcaa
     2581 ccaccgcacc agcgacggcc agccgacccc gaccctgacg gccggcgagg acgagctgac
     2641 cggcatcgcg ctcggcctca aggcggccgg caagggcgtg ctgcagttcg tgtccgactt
     2701 ccagaacccg gccgaggagt tcgccatgtt ccgccggctg gtcgaggcgt cgggccggcc
     2761 gctgagcttc tccctggccc agagcgcgcg cggcaaggac gcctggaagc tgctgctggc
     2821 ggccctgtcg gatgcggtcg actccggcct gccgatgaag gcccaggtgg ccggccgtcc
     2881 cgtgggggtg ctgttcgggc tggaactgac gctcaacccg ttcagccagc acgagaccta
     2941 cagggaaatc gcccacctgc cgctggccca gcgggtggcg catctgtccg atccggcctt
     3001 ccgcgcgcga ctgctcagcg aacagctcga cccgaagggc ccgttcgccg gcagcgccct
     3061 gcgcgcctgg gacaacatgt tcctgatgac cgaggcgccc gaatacgagc cgacccagga
     3121 ccagacggtc aaggcgctgg ccgccggccg gggcctgtgg ccggacgagg tggcgctgga
     3181 ccacatgctg tcgaacggcg ggcggggcat gctctaccat ccgttcctca actacgccga
     3241 tggttcgctg gaccccagtt tcgccatgct gagtcatcgc gacacggtgc cgggcctcag
     3301 cgacggcggg gcccatgtcg ggatgatctg cgatggcagc ttcccgacct cgaacctgac
     3361 ccactggacc cgcgaccgca cgcgcgggcc gaagctggcg ctggaggagg tcattcgcat
     3421 gcagacgcgc gacacggccg cggcggtcgg cctgctggac cggggccaga tcgccgtggg
     3481 gctgcgggcc gacctcaatg tcatcgacta cgacggcctg accctgcacg cgccgggcgt
     3541 tgcctacgac ctgccggccg gcgggcggcg gctggtgcag cgggccgacg gctatgtcgc
     3601 gacgatcgtg gccggccaga tcacctaccg tgacggcgag cccacggggg ccctgccggg
     3661 gcggctggtg cgcggcgcgc ggtcggcgcc ggtggcgatg gcggcggagt agggtccaac
     3721 cccgcgtcct tcgacacggc gcttcgcgcc tgctcaggat gacgtggagg gggggcaaaa
     3781 tccacgtcgg ggtgagcagc gctcgaagag cgcgtgtcca accacgcgct ctactccccg
     3841 aacccctcgc cgagctcggt gatcggggcc atgcggatca ggcggctgtc ctcgacgccg
     3901 gcctggcggt gcttgaccac ctcgcggtac tcgggatcgg tgaccatggc caggaacgcc
     3961 ccgccgccag ggtattcggc gatgaaggcg atgtcccagc gctcgtcggt cggtccggtg
     4021 acgaccacgt cgggccgccc ggcccagacc tgccggccgc cgacccgctt gaagatgtgc
     4081 gcgctttcac ggccgtaggc gcggtaggcc tccaggccgg tcatgccctt gccgtggttg
     4141 ggatggccct cgggatagtc ggccagggcg cgaacccgca cgaggttgag catgtggatc
     4201 ggttcgtcgc ggggcagggc cttgaaggcg tcgaactgcg ggcgggtggg gtcgatggag
     4261 ccggtcatgg ggggagtgtt caccggcgaa ggcgacttgg ctagtcggtc gtcattcctg
     4321 gcaggccgcg ccctgatgtc ttgatgagca gttttgctcc ggcgggacct tcgtcaagga
     4381 cgggcccgca gggcccgccg ctg
//
LOCUS       DAORNX010000245         4401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14840_length_4401_cov_7.991947, whole genome shotgun sequence.
ACCESSION   DAORNX010000245 DAORNX010000000
VERSION     DAORNX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14840_length_4401_cov_7.991947"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLO65_10940"
     CDS             complement(<1..244)
                     /locus_tag="PLO65_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28804.1"
                     /translation="MTHFLSRTALVALLGAAATACASTPAYPTVEGVSPGTGPQPARP
                     AYPVQPPAQTDEMGDPVPDAAPSTPVDAGVLPPAGPP"
     gene            complement(346..1002)
                     /locus_tag="PLO65_10945"
     CDS             complement(346..1002)
                     /locus_tag="PLO65_10945"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRD28805.1"
                     /translation="MTDEPETPQTRMARLILALRSQGVTDARVLDAIERTPRELFTPD
                     LFKERAFEDSALPIACGQTISQPFIVGLMTQALTLGPRDRVLEIGTGSGYQAAVLSRL
                     ARLVYTVERYRTLMKEAEERFRTLGLTNVITRFGDGGEGWAEQAPFDRILVTAAAPEE
                     PKALLAQLKPGGVLVAPIGKGPVQSLRRYAGDGQGGFKVELLGDVRFVPLLDGVAREL
                     "
     gene            complement(1002..1784)
                     /gene="surE"
                     /locus_tag="PLO65_10950"
     CDS             complement(1002..1784)
                     /gene="surE"
                     /locus_tag="PLO65_10950"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRD28806.1"
                     /translation="MRILLTNDDGIHAEGLRSLETIARALTDDVWIVAPEYEQSGASR
                     ALTLADPIRVRREAERRWAVAGTPTDCVMLGVRELIEGAAPDLVLSGVNRGQNIAEDV
                     TLSGTVAGAIEGMALGIPSIALSQAMLAFHDEVIPFWETAEAFGPGVVRRLLEVGWPA
                     DVVMNVNFPSRPPGDVTEIEVTRQGFRDTHIRTFDKRTDLRGRDYYWMGFSGQMSRPA
                     EGTDLKAIYDGKISITPLHIDLTHRESVNNLKGLIGGVPPKA"
     gene            complement(1784..2029)
                     /locus_tag="PLO65_10955"
     CDS             complement(1784..2029)
                     /locus_tag="PLO65_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28807.1"
                     /translation="MLRTWDIVIVSPDFEDEPIRGKRGHICSLVDADGFAVLIHDLER
                     VWCLGHDDVTATGEQLPEAERPGGRSIRVNQKGEIVG"
     gene            complement(2029..3309)
                     /gene="serS"
                     /locus_tag="PLO65_10960"
     CDS             complement(2029..3309)
                     /gene="serS"
                     /locus_tag="PLO65_10960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRD28808.1"
                     /translation="MHDIKAIRDNPAFYDRGWASRGLGPQTPTLLALDRDLRAAQTAV
                     QTAQSRRNEASKLIGQAKAQKDETRAAELMAQVEALKAEMAEAAEAEVRLGKDLRDLL
                     AALPNIPDAVVPPGEDETGNLEQDRHGDPRRMNNARDHVDIGAGLGGMDFEAAARMSG
                     SRFVVLKKGVARLERAIGQFMLDLQTGTNGYTEVSPPVLVKSDALFGTGQLPKFEEDL
                     FRTTGEHWLIPTAEVSLTNLVREQITDEEELPLRVTALTHCFRSEAGSAGRDTRGMIR
                     QHQFQKVELVAITTPEQSAAEHDHMVASAQMVLEKLGLAYRTMLLCAGDMGFTARKTF
                     DLEVWLPSQNTFREISSISNCGDFQARRMDARCQKRGEKGTRFVHTLNGSGLAVGRTL
                     VAVLENYQDEGGRVFVPEVLRPYMGGLTHLEPEA"
     gene            complement(3415..4308)
                     /gene="tatC"
                     /locus_tag="PLO65_10965"
     CDS             complement(3415..4308)
                     /gene="tatC"
                     /locus_tag="PLO65_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRD28809.1"
                     /translation="MSDDLTPDEAEIEASRAPLLDHLVELRRRLIVCVAALIVGFGVC
                     FAFADNIYTFLLEPFVKAGALIAAQHAGLKHGPFDLIEVMVGLKEAPDLNKKTLELIF
                     TAPLEFFFTKMKMAGFGAVVLTFPILAWQLYAFVAPGLYRKERRAFLPFLAASPVLFT
                     MGAALVYYIMLPFVMWFSLSQQVTTATVNVALVPKVSDYLTLVTTLLLAFGLCFQLPV
                     VLSLAGLAGLVNSKLLREGRRYAIVVVFVIAAIFTPPDPISQLMLAIPICLLYEASIW
                     CVWLIERRRKRDEAGTDIVVA"
BASE COUNT          683 a         1496 c         1538 g          684 t
ORIGIN      
        1 ccggcgggcc cgccggcgga aggacgccag cgtcaaccgg cgtcgacggc gcggcgtcag
       61 ggaccgggtc gcccatctca tcggtctggg ccgggggctg caccggatag gcgggacgcg
      121 ccggctgcgg acccgtgccg ggactgaccc cctccaccgt cggataggcc ggcgtcgagg
      181 cgcaggcggt cgccgccgcg ccgagcaggg cgacaagggc ggtgcgggag agaaaatgcg
      241 tcatcgccgg tccttcggta aaccccgatt gtgtcgtccc agccgtctta aacctgcgtt
      301 aaccgcagcc tcgaagccag ttacgatatt gtcagagcca ggggctcaga gttctcttgc
      361 cacgccgtcc agcagcggca cgaagcgcac atcgccgagc agctcgacct tgaatccgcc
      421 ctgcccgtcg ccggcgtagc ggcgcaggct ctgcaccggg cccttgccga tcggcgcgac
      481 cagaacgccg cccggcttga gctgggcgag cagggccttc ggctcctcgg gcgcggcggc
      541 ggtgaccagg atgcggtcga acggagcctg ctcggcccag ccctcgccac cgtcgccgaa
      601 gcgggtgatg acattggtca ggccgagcgt gcggaaccgc tcctcggcct ccttcatcag
      661 ggtgcgatag cgctcgaccg tatagaccag ccgcgccagc cgcgagagaa ccgccgcctg
      721 gtagcccgaa ccggtgccga tctcgagcac ccggtcgcgc ggacccaggg tcagggcctg
      781 ggtcatcaga ccgacgatga atggctggct gatcgtctgg ccgcaggcga tcggcagggc
      841 cgagtcctcg aacgcccgct ccttgaacag gtccggcgtg aacagttcgc gcggcgtgcg
      901 ctcgatggcg tcgagcacgc gagcgtctgt caccccctgg gaccggaggg cgaggatcag
      961 gcgcgccatg cgcgtctgcg gggtctctgg ctcgtcggtc atcaggcctt cggcgggacg
     1021 cctccaatga ggcccttgag gttgttcacc gattcccggt gcgtcaggtc gatgtgcaac
     1081 ggcgtgatcg agattttgcc gtcgtagatc gctttcaggt cggttccctc ggccggccgg
     1141 gacatctgcc cggaaaagcc catccagtag tagtcgcggc cgcgcaggtc ggtgcgcttg
     1201 tcgaaggtgc ggatgtgggt gtcgcggaaa ccctggcggg tcacctcgat ctcggtgacg
     1261 tcgcccggcg gccgcgaggg aaagttgacg ttcatcacca cgtcggccgg ccagcccacc
     1321 tcaagcaggc ggcggacgac gcccgggccg aacgcctcgg cggtctccca gaacggaatg
     1381 acctcgtcat gaaaggccag catggcctgg ctcagcgcga tcgagggaat gcccagggcc
     1441 atgccctcga tggcccccgc gaccgtaccc gacagggtca cgtcctcggc gatgttctgg
     1501 ccgcggttga cgcccgacag caccaggtct ggggccgcgc cctcgatcag ttcgcgcacg
     1561 cccagcatga cgcagtcggt gggcgtgccg gccacggccc acctgcgctc ggcctcgcgg
     1621 cgcacccgga tcggatcggc cagggtcagg gcccggctgg cgcccgactg ctcgtattcc
     1681 ggcgcgacga tccagacgtc atctgtcagg gcccgggcga tggtctccag cgaccggagc
     1741 ccctcggcat ggatgccgtc gtcattggtc agcaggatgc gcatcagccg acaatctcgc
     1801 ccttctggtt gacgcggatc gaccggccgc ccggtcgttc ggcctcgggc aactgctcgc
     1861 ccgtcgcggt cacgtcgtcg tggcccaggc accagacgcg ctcgagatca tggatcagca
     1921 cggcgaaacc gtccgcgtcc accagcgaac agatgtggcc gcgcttgccg cggatcggct
     1981 catcctcgaa gtcgggcgag acgatcacga tgtcccaggt gcgcagcatc aggcttccgg
     2041 ttccagatgc gtgagcccgc ccatgtaggg ccgcaggacc tcggggacga acacccggcc
     2101 gccctcgtcc tggtagttct caagcaccgc caccagggtc cggccgacgg ccaggcccga
     2161 tccgttcagg gtgtggacga accgcgtgcc cttctcgccg cgcttctggc agcgcgcgtc
     2221 catccggcgg gcctggaagt cgccgcagtt ggagatcgag ctgatctcgc ggaaggtgtt
     2281 ctgcgacggc agccagacct ccaggtcgaa ggtcttgcgg gcggtgaagc ccatgtcgcc
     2341 ggcgcacagc agcatggtgc ggtaggccag gcccagtttc tccagcacca tctgggcgct
     2401 ggcgaccatg tggtcgtgct cggccgccga ctgctcgggc gtggtgatcg ccaccagctc
     2461 gaccttctgg aactggtgct gacggatcat gccgcgcgtg tcgcgccccg ccgaaccggc
     2521 ttccgagcgg aagcagtggg tcagggcggt gacgcggagg ggcagttcct cctcgtcggt
     2581 gatctgctcg cggacgaggt tggtcagcga cacctcggcg gtggggatga gccagtgctc
     2641 gcccgtcgtg cggaacaggt cttcctcgaa tttcggtagc tggccggtcc cgaaaagcgc
     2701 gtccgacttg accagcactg gcgggctcac ctcggtgtag ccgttggtcc cggtctgcag
     2761 gtcgagcatg aactggccga tggcccgctc gagccgggcg acgcccttct tcagcacgac
     2821 gaagcgcgag ccgctcatcc gcgcggcggc ctcgaaatcc atgccgccca ggcccgcgcc
     2881 gatgtcgacg tggtcgcggg cgttgttcat ccggcgcggg tcgccatgac gatcctgctc
     2941 gaggttgccg gtctcgtcct cgcccggcgg cacgaccgcg tcggggatgt tcggcagggc
     3001 ggccagcagg tcgcgcaggt ccttgcccag ccggacctcg gcctcggccg cctcggccat
     3061 ctcggccttc agggcctcga cctgggccat cagctcggcg gcccgggtct cgtccttttg
     3121 cgccttggcc tggccgatca gcttgctggc ctcgttgcgg cgcgactggg cggtctgcac
     3181 ggccgtctgg gcggcgcgca ggtcccggtc gagcgccagc agggtcggcg tctgcggccc
     3241 aaggccacgg gacgcccagc cgcggtcgta gaacgcgggg ttgtcgcgaa tggctttgat
     3301 atcgtgcatg ggaagaacgc cggttgcggt gaggcgcttt ctctaacggg cgtcgggggg
     3361 atttagaagc cctgccgcca ctgcatctcc ctctcgggag agggggagga aagactacgc
     3421 caccacgata tccgtgcccg cctcgtcccg cttccggcgg cgctcgatca gccacacgca
     3481 ccagatcgag gcctcgtaga gcaggcagat cgggatcgcc agcatcagct ggctgatcgg
     3541 gtcgggcggg gtgaagatgg cggcgatgac gaacaccacc acgatggcgt agcggcgccc
     3601 ctcgcgcagg agcttgctgt tgaccagccc cgccagcccc gccagcgaca gcaccaccgg
     3661 cagctggaag cacaggccaa aggccagcag cagcgtagtg accagcgtca ggtagtcgga
     3721 caccttgggc accagggcga cattgaccgt cgcggtggtc acctgctggc tcagggagaa
     3781 ccacatcacg aacggcagca tgatgtagta gaccagcgcc gcacccatgg tgaacagcac
     3841 cggcgaggcg gccaggaacg gcaggaaggc ccgtcgttcc ttgcgataaa ggcccggagc
     3901 cacgaaggcg tacagctgcc aggccaggat cgggaaggtc agcaccacgg ccccgaagcc
     3961 ggccatcttc atcttggtga agaagaactc cagcggcgcg gtgaagatca gctcaagcgt
     4021 cttcttgttg aggtccggcg cctccttgag gccgaccatc acctcgatga ggtcgaacgg
     4081 gccgtgcttg agacccgcat gctgggccgc gatcagggcc ccggccttga cgaacggctc
     4141 cagcaggaag gtgtagatgt tgtcggcgaa ggcgaagcag acgccgaacc cgacgatcag
     4201 ggccgcgacg cagacgatca ggcggcggcg cagctcgacc agatggtcca gcagcggcgc
     4261 gcgcgaggcc tcgatctcgg cctcgtccgg tgtcaggtcg tcgctcacga cggcgtctcc
     4321 gccttcttgc gcggggcccg cggcttgcgg gcccttgggg cggcctcggc gggcgggtcg
     4381 ccggcgggcg aagccgcttt c
//
LOCUS       DAORNX010000246         4390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14877_length_4390_cov_6.288120, whole genome shotgun sequence.
ACCESSION   DAORNX010000246 DAORNX010000000
VERSION     DAORNX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14877_length_4390_cov_6.288120"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLO65_10970"
     CDS             complement(<1..400)
                     /locus_tag="PLO65_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRD28810.1"
                     /translation="MSIDLPAPMAAAFRACRPHFIGIAIFSGLLNLLFLTPSIYMLQV
                     YDRVVPTGGLLTLAFITVAVAFALATLAALDALRARLLVRASLRLDRMLAGPLLERLM
                     ARAGPGAPVLGMREFDILRQALSGPAALGFL"
     gene            complement(482..775)
                     /locus_tag="PLO65_10975"
     CDS             complement(482..775)
                     /locus_tag="PLO65_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28811.1"
                     /translation="MIDLPGPQQPGRVAAAGDAEPPPGLVEVAIDGMLRNPQAAGDFL
                     GVQVLGDQPETLPLARGQALDRRWVVLLPHDRRGKSPARLSSIPLVNPATTRH"
     gene            870..1190
                     /locus_tag="PLO65_10980"
     CDS             870..1190
                     /locus_tag="PLO65_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28812.1"
                     /translation="MTEIRDRDEAAFDIGTPPIIEPATEPELWNAAADMPVWTWSQLE
                     DALAELAASRQQHDMIENLVSATRKQARYLPSGHILKEILCIAWVIADETFRGGREEG
                     SSMT"
     gene            1406..1762
                     /locus_tag="PLO65_10985"
     CDS             1406..1762
                     /locus_tag="PLO65_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28813.1"
                     /translation="MQRKIMGHRIAAQLIAAEAAIDGALSAVATLTAMLPTARIESRL
                     SAVVGQGVFDRSSQTILALTEARRGIVETHRELSGVQHQIGLGGVALGGEDKPDETTP
                     PKEMRLRTVTSGRTAA"
     gene            1835..2254
                     /locus_tag="PLO65_10990"
     CDS             1835..2254
                     /locus_tag="PLO65_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28814.1"
                     /translation="MTPASQIGAIVMALVCLFAGWAGGRPQRVVAAITFAAWVLSAAI
                     QDRSYRHPQYATFVFDAALLVVFVWLALLWKRGWLNWVAAFQSLTMATHIAAMLDRRI
                     WPIASITAYMIWSYLTLAAVGWGGVQGLLERRRARRG"
     gene            complement(2251..>2518)
                     /locus_tag="PLO65_10995"
     CDS             complement(2251..>2518)
                     /locus_tag="PLO65_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28815.1"
                     /translation="IDPNNPPPGALAPRAGDTAPAVPPSAQPAAEKGADATGQIALWA
                     FLTMLLGAVGAIAGSRYGARRHGWEARMKMTDDPAVRVSPGGVI"
     assembly_gap    2519..2528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2529..2997)
                     /locus_tag="PLO65_11000"
     CDS             complement(<2529..2997)
                     /locus_tag="PLO65_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28816.1"
                     /translation="MTDIQPTAYEPHPLHTLVSWPAVIAGAVVAVAVGAMLNLLGVAI
                     GAAALNPFDLSRDDAQAFSVGAGVWVALANAAALFAGAMLASRAAKYSDHHRGLLQGL
                     SVWAVAFLMAIFIAGSTAAGGLTAVLSGVADEADVDVAAAPAPPVYYEKGGPMV"
     gene            complement(3078..3887)
                     /locus_tag="PLO65_11005"
     CDS             complement(3078..3887)
                     /locus_tag="PLO65_11005"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004857.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD28817.1"
                     /translation="MSYQDILYQVEDGVAVVTLNRPDRLNAWTGRMHVEVKAAMSAAT
                     DDPAVKVIILTGAGRGFCAGADMNALQDIGSGEREAAPDEPFDPSAPEDFRKTYSYFP
                     SVPKPIIGAINGACAGLGMVMALYTDIRFAADTAVFTTAFSRRGLIAEHGISWLLPRL
                     VGFAQAADLLFSARRVDAAEAERIGLVNRIMPADGFMDTVMAYARMLATEVSPRSMRV
                     MKREIWAAQFQGLGEAIETANADMQASFASADFKEGVAHFVEKRAPAFTGL"
     gene            3948..>4390
                     /locus_tag="PLO65_11010"
     CDS             3948..>4390
                     /locus_tag="PLO65_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD28818.1"
                     /translation="MKGVAFGRTTYETLLCDRPAAGVARITLNRPAAMNAYSFGMTQE
                     LQEAIASFRDDDALKVLVLTGAGERAFCTGGDISGNDPQHAAMVAAAPMGHGREMREG
                     MQAVVLALRRLDKPSIAMVRGYAVAGGLALACACDFRFAAGTARLG"
BASE COUNT          670 a         1536 c         1556 g          618 t
ORIGIN      
        1 cgagaaagcc cagcgccgcc ggacccgaca gggcctgacg caggatgtcg aactcgcgca
       61 tgcccagcac cggggcgccg gggcccgcgc gggccatcag gcgctcgagc agcggcccgg
      121 cgagcattct gtccagccgc aggctcgcgc ggaccagcag ccgggcgcgc agcgcgtcga
      181 gagccgccag cgtggccagg gcgaaggcca cggccacggt gatgaacgcc agggtcagca
      241 ggccgccggt cggaacgacg cggtcgtaga cctgcagcat gtagatcgac ggtgtcagga
      301 acagcaggtt gagcaacccg ctgaagatcg cgatgccgat gaaatgaggc cgacaggctc
      361 ggaatgccgc ggccatcggc gcaggcagat cgatactcaa gaacgtcacg ggcggccctg
      421 catgggattg cgcctgtcgc gcgagccgag cttaagccac gcggccagat cggccaatgc
      481 gtcaatgacg tgtggtcgca gggttaacaa gggggatgga cgaaagcctc gccgggcttt
      541 tacctctgcg gtcatggggg agcagcacga cccaccggcg atcgagcgcc tgaccccgcg
      601 cgagcgggag tgtctccggc tggtcgccga gcacctgcac tccaaggaaa tcgcccgccg
      661 cctgcggatt tcgcagcata ccgtcgatgg ccacctcaac gaggcccggc ggcggctcgg
      721 cgtcgccagc cgccgcgacg cggcccggtt gctgcgggcc tgggaggtcg atcatccccc
      781 cctcaatggt ttagggggcg ccccgccggg gatggtcctg ctgcccgatg acccgtcagc
      841 cttgtcgcag ttgttgcggg aggcggacga tgaccgagat cagggacagg gacgaggcgg
      901 ccttcgatat tgggacgccg ccgatcatcg agcccgcgac ggagcccgag ctctggaacg
      961 cggcggcgga catgccggtc tggacctgga gccagctgga ggacgccctg gccgaactgg
     1021 cggcgagccg tcaacagcac gacatgatcg agaacctggt ctcggcgacg cgcaagcagg
     1081 cccggtacct cccctcgggg cacattctca aggagatcct ctgcatcgcc tgggtgatcg
     1141 ccgacgagac gttccggggc ggccgggagg agggttcctc gatgacctag cgcccttccg
     1201 ccagatcgcg ttcatcggcg ccgcggccgt cctcgccgga ctgttgctca gcggcgtgat
     1261 catcgccagc caccagttcg cctacgtcat ccagcagctg ttcgccggct gggactgaac
     1321 tctccgggcc agcgcccgcc tattcctgaa aacccaagca caaccggcgc gtcggcattc
     1381 ccggcgcgga cgggaggagt ctgtcatgca acgcaagatc atgggccacc ggatcgccgc
     1441 gcagttgatc gcggccgaag cggccatcga cggcgccctg tcggccgtcg ccacgctgac
     1501 cgccatgctg cccacggccc gcatcgagtc gcgactctcg gccgtcgtcg gacagggcgt
     1561 gttcgaccgc tcgagccaga ccatcctggc cctgaccgag gcccgtcgcg gcatcgtcga
     1621 gacccatcgc gaactgtccg gggtgcagca ccagatcggc ctgggcgggg tggccctggg
     1681 cggggaggac aagcccgacg agaccacgcc gcccaaggaa atgcggctca ggaccgtgac
     1741 gtcgggccgc accgccgcct gacaccctta actcgccgcc caaatcgggg tgtgatggcc
     1801 ggagctaacc gcagggtgtg ttccggccat gttcttgact cccgcctccc agatcggggc
     1861 catcgtcatg gcgctggtct gcctgttcgc gggctgggcc ggcgggcggc cgcagcgcgt
     1921 cgtcgccgcc atcaccttcg ccgcctgggt gctgtccgcg gccatccagg accgcagcta
     1981 ccgccatccg cagtacgcca ccttcgtgtt cgacgcggcc ctgctggtcg tcttcgtctg
     2041 gctggccctg ctgtggaagc gcggctggct caactgggtc gccgccttcc agagcctgac
     2101 catggcgacc cacatcgccg ccatgctcga caggcgcatc tggccgatcg cctcgatcac
     2161 cgcctacatg atctggagct acctgactct cgccgccgtg ggctggggtg gcgtccaggg
     2221 cctgctggag cgacgccggg cccggcgcgg ctagatcacg ccgcccggcg aaacccggac
     2281 cgccggatcg tcggtcatct tcatccgcgc ctcccagcca tggcgacggg cgccgtagcg
     2341 cgagcccgcg atcgcgccga cggccccgag cagcatggtg agaaaggccc acagggcgat
     2401 ctggccggtg gcgtcggcgc ccttttcggc agcgggctgg gccgaaggcg gaacggccgg
     2461 agcggtgtcg cccgcacgcg gggccagcgc gcccggcggg gggttgttcg ggtcgatcnn
     2521 nnnnnnnngc accatcggcc cgcccttctc gtaatagacc ggcggcgcgg gcgccgcggc
     2581 gacatcgacg tccgcctcgt cggcgacccc gctgagcacc gcggtcagcc ctccggcggc
     2641 ggtcgatccg gcgatgaaga tggccatcag gaaggccacc gcccagaccg acaggccctg
     2701 cagcagaccg cgatgatggt cgctgtactt ggccgcgcgg ctggccagca tggccccggc
     2761 gaacagcgcc gccgcgttgg ccagggcgac ccacaccccg gcgccgacgc tgaacgcctg
     2821 ggcgtcgtcg cggctgagat cgaatggatt gagcgccgcg gcgccgatgg cgaccccgag
     2881 caggttgagc atggcgccga ccgcgacggc gacgaccgcc ccggcgatca cggccggcca
     2941 ggacacgagc gtatgcagcg ggtggggttc ataggcggtg ggctggatgt cggtcacggc
     3001 gtctgcctcc tgcgcggacg tcgatgcgcg tcctcgcagg cgacaacgtt cggcgaaccg
     3061 gatcgttcgc ctgggcgcta gaggccggtg aaggccgggg cgcgcttctc gacgaaatgg
     3121 gccacgcctt ccttgaagtc ggccgaggcg aagctggcct gcatgtcggc gttggccgtc
     3181 tcgatggcct cgccgaggcc ctggaactgc gccgcccaga tctcccgctt catcacccgc
     3241 attgaacggg gcgagacctc ggtggccagc atccgggcgt aggccatcac cgtgtccatg
     3301 aagccgtcgg ccggcatgat ccggttgacc aggccgatcc gctcggcctc cgccgcatcg
     3361 acccggcgcg ccgagaacag caggtcggcg gcctgggcga agccgaccag ccggggcagc
     3421 agccagctga tgccgtgttc ggcgatcagc ccgcgccgcg agaaggcggt ggtgaacacg
     3481 gccgtgtccg ccgcgaagcg gatgtcggtg tagagggcca tgaccatgcc caggcccgcg
     3541 caggcgccgt tgatcgcccc gatgatcggc ttgggcacgc tggggaagta agaataggtc
     3601 ttgcggaagt cctccggcgc cgacggatcg aacggctcgt ccggcgcggc ctcgcgctcg
     3661 ccggagccga tgtcctgcag cgcgttcatg tcggccccgg cgcagaagcc gcggcccgcg
     3721 ccggtgagga tgatcacctt cacggccgga tcgtcggtcg ccgcgctcat ggccgccttg
     3781 acctcgacat gcatccggcc ggtccaggcg ttgagccggt cgggccggtt cagcgtgacc
     3841 acggcgacgc cgtcctcgac ctgatacaga atgtcctgat agctcatgcc ggcctcccgt
     3901 tccgtgtcgg cgggaagcct agctcaagcg atacgggagt cgaggcgatg aagggcgtgg
     3961 cgttcggacg gaccacctac gagaccctgc tctgcgaccg gccggcggcg ggcgtggcgc
     4021 ggatcaccct gaaccggccg gcggcgatga acgcctacag cttcggcatg acccaggagc
     4081 tgcaggaggc gatcgccagc ttccgcgacg acgacgccct gaaggtcctg gtgctgaccg
     4141 gcgccggcga gcgggccttc tgcaccggcg gcgacatctc ggggaacgat ccgcagcacg
     4201 ccgccatggt ggccgcggcg ccgatgggcc atggccgcga gatgcgcgag ggcatgcagg
     4261 cggtggtgct ggccctgcgc cggctggaca agccctcgat cgccatggtg cgcggctacg
     4321 cggtggccgg cggcctggcc ctggcctgcg cctgcgactt ccgtttcgcc gccgggaccg
     4381 cccgcctggg
//
LOCUS       DAORNX010000247         4382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14916_length_4382_cov_6.603420, whole genome shotgun sequence.
ACCESSION   DAORNX010000247 DAORNX010000000
VERSION     DAORNX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14916_length_4382_cov_6.603420"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLO65_11015"
     CDS             <1..639
                     /locus_tag="PLO65_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HRD28819.1"
                     /translation="GQPLIWRQLERLKAARTLTRIVVATSCEAADDPLAAWLVSQGQA
                     VFRGAPADLLDRFARCAVSAGPAAHVVRIKGDAPFVDPRVIDEAVRMARVSGADYVSN
                     REPASYPRGMEVEVIATPALVAAAAEPREIRARVSPTAFIRANPGRFSQASFSHHRDL
                     SRLDWRVKTAADLAFARSIYGALHAADPAFGMADVLDLVGGRQDLARFSYAA"
     gene            complement(623..2449)
                     /locus_tag="PLO65_11020"
     CDS             complement(623..2449)
                     /locus_tag="PLO65_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD28820.1"
                     /translation="MKRLLLTTTALLALTTTARAETASPVPVAELIVTATRLETGIDA
                     APDARVITADDIIASQATFAGDILETIPGLSLSRNGDFGGVTYVRMRGAGADKTLVLI
                     DGVVQNDPSSPNGGFDFSSLDLADVERIEVLSGPLGSLWGSDAIGGAIAFTTRETDGW
                     RASVEAGSFGVARATVAAGVADDRRALGLTAAAYAGDGVSKAAVGTEADGFDSWTVGA
                     NARLDLTDRVRIDGRLRYNEAKVALDGYDAFFVFGDTADRSESRSWTGFARARADGLL
                     GLDQTLSVSLYDLVRDSISAFPSSYDAQRTTWRWTGGRGAPGDVFAFLVGLERDDTEA
                     SISTGATSDLGVTSAFGVVRLSPVAPLTVTASLRHDAPDAYDGKITARLAGVLALDHG
                     LLLSASIGQGFKTPTISQTVCDFCFPPGPSTGLRPETAEGWDAALSWRSADDRFYARV
                     TGYRLEVKDQISYGVGRYVNIDRTRTTGVEVELDADLTDRVALRASYAFSDAVDESTG
                     AELIRAPEQSGSLSLLWTGDRLRAALAVRAEGEQADSDPSTFAPARRGGFVVANLSGS
                     WALSDRIDLTARVTNLTDEAYQQSLGYGEAGRGLFVGFRLRN"
     regulatory      complement(2587..2789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2969..4081)
                     /gene="ald"
                     /locus_tag="PLO65_11025"
     CDS             complement(2969..4081)
                     /gene="ald"
                     /locus_tag="PLO65_11025"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRD28821.1"
                     /translation="MRVGVPAEIKPGEHRVGLTPTAVREYVAHGHQVLVEAGAGQGAG
                     YADDAFRKAGATIVADADAVFSGADMIVKVKEPQKVEWERLEPRHILFTYLHLAPDPA
                     QTEGLLASGCAAIAYETVTDARGGLPLLAPMSEVAGRIAVFSAAETLLKHNGGMGLLL
                     CGVPGVSPARVAVLGGGVVGMNAARMAMGLNAEVVVLERSIPRMRELDDIFMGRVLTR
                     YSTLDAVEDEILKADVVIGAVLTAGAAAPKLVKREHLSRMKPGSVLVDVSIDQGGCFE
                     TSHPTTHAEPTYVVDGVVHYCVANMPGAAPRTSSEALGNATLPFGLALADHGLDALKR
                     DRHLAKGLNVLKGELTHPAVAEALGKPFVDPYGAWA"
BASE COUNT          615 a         1560 c         1558 g          649 t
ORIGIN      
        1 ggccagccgc tgatctggcg tcagctggaa cgcctcaagg ccgcccggac cctgacccgc
       61 atcgtcgtgg ccaccagctg cgaggcggcc gacgatccgc tcgccgcctg gctcgtgtcc
      121 cagggccagg ccgtgttccg cggcgctccg gccgacctgc tcgaccgctt cgcccgctgc
      181 gccgtctcgg ccggcccggc cgcccacgtc gtgcgcatca agggcgacgc gccgttcgtc
      241 gacccccgcg tgatcgacga ggccgtgcgc atggccaggg tcagcggggc cgactacgtc
      301 tccaaccgcg agccggccag ctatccgcgt ggaatggagg tcgaggtgat cgccaccccc
      361 gcccttgtcg ccgccgcggc cgagccgcgc gagattcgcg cccgggtgtc gccgaccgcc
      421 ttcatccgcg ccaacccggg tcgcttctcg caggcgagct tcagtcatca ccgcgatctg
      481 tcgaggctcg actggcgggt gaagacggcc gccgacctgg ccttcgcccg gtcgatctac
      541 ggcgccctgc acgccgccga cccggctttc ggcatggccg atgtgctcga cctggtcggc
      601 gggcgccagg atctggcgcg gttcagttac gcagcctgaa gccgacgaac aggccgcggc
      661 ccgcctcgcc gtagccgagc gactgctggt aggcctcgtc ggtgaggttc gtcacgcggg
      721 ccgtcaggtc gatcctgtcg ctgagggccc agctgcccga caggttcgcc accacgaacc
      781 cgccgcgccg cgcgggcgcg aaggtcgagg ggtctgaatc ggcctgctcg ccctcggccc
      841 gcaccgccag cgcggctctg agccggtcgc ccgtccacag cagacttagc gatccggact
      901 gctccggcgc ccggatcagt tcggcgcctg tcgactcgtc gaccgcgtcg ctgaaggcgt
      961 aggacgccct gagcgccacg cgatcggtca ggtcggcgtc cagttcgacc tcgacccccg
     1021 tggttctcgt gcggtcgatg ttcacatagc ggccgacgcc gtagctgatc tggtccttca
     1081 cctcgagccg gtagccggtg acccgggcgt agaacctgtc gtcggccgat cgccaggaca
     1141 gggccgcgtc ccagccctcg gcggtctccg gcctgagccc cgtcgacggc ccgggcggga
     1201 agcagaagtc gcagacggtc tggctgatgg tcggggtctt gaacccctgg ccgatgctgg
     1261 ccgacagcag caggccgtgg tccagcgcca gaaccccggc cagccgggcc gtgatcttgc
     1321 cgtcgtaggc gtccggcgcg tcgtggcgca ggcttgcggt gacggtcaga ggcgcgacgg
     1381 gggagagccg caccacgccg aaggccgacg tcaccccgag gtccgacgtg gcgcccgtgc
     1441 tgatcgaggc ctcggtgtcg tcacgctcga gtccgacgag gaaggcgaag acatcgcccg
     1501 gcgcaccccg gccgccggtc cagcgccagg tcgtccgctg cgcatcgtag ctggatggaa
     1561 aggccgagat gctgtcgcgg accaggtcgt agaggctgac gctgagcgtc tggtccagac
     1621 ccagcagacc gtcggcgcga gcgcgggcga aaccggtcca gctgcggctc tccgagcggt
     1681 cggctgtgtc gccgaagacg aagaaggcgt cgtagccgtc cagggcgacc ttcgcctcgt
     1741 tgtagcgcag ccggccgtcg atccgtacac ggtcggtcag gtccagccgg gcgttggcgc
     1801 cgacggtcca gctgtcgaat ccgtcggcct ccgtgccgac cgccgccttg gaaaccccgt
     1861 cgccggcgta ggcggcggcg gtaaggccca gggcgcggcg atcatcggcg acaccggcgg
     1921 cgacggtcgc ccgcgccacg ccgaacgacc cggcctcgac ggaggcgcgc cagccgtcgg
     1981 tctcgcgcgt ggtgaaggcg atcgcaccgc cgatcgcgtc cgagccccac agcgacccca
     2041 gcggccccga cagcacctcg atccgctcga cgtcggccag gtcgaggctg gagaagtcga
     2101 agccgccgtt cggactggac gggtcgttct ggaccacgcc gtcgatcagc acgagggtct
     2161 tgtcggcgcc ggcgccgcgc atgcgcacat aggtgacgcc gccgaagtcg ccgttgcggc
     2221 tgagcgacag gcccgggatg gtctccagaa tgtcgcccgc gaaggtcgcc tggctggcga
     2281 tgatgtcgtc ggccgtgatg acccgggcgt ccggggccgc gtcgatgccg gtttcgaggc
     2341 gcgtggcggt gacgatcagc tcggcgacgg ggaccggcga ggcggtctcc gcgcgggctg
     2401 tcgtggtcag ggccagcagg gcggtggtgg tcagcagaag gcgcttcatg gcgcaggtcc
     2461 tcacgactgt gagcccgcgc ggcgccgcgt ggcgaaggtg tccaaagcga acgtcgacgc
     2521 gccggccgac ggtcgcttca acgggaaggc aggccttccc cgcgccacca gcctggaccc
     2581 gggctgcgga cgagcgacgg cggcggcagg tctcctggct cgcgggtctg caggtcgcgt
     2641 cctcgccttc ccggtttccc agtggcgtcc ccttcgtttg aagaggcgcg gacgcggccc
     2701 tcgccgctga cagttgcggg cacagccggg gcttctcacc cctgttccct cttggccccg
     2761 atatggggac cgcctgggcg cgttatggtt gggtgacgtg ctgtgcgcaa gaacccttct
     2821 ccccttgcgg gagaaggtgt cgtcgaagcc gacggatgag ggggctcacg aacaggcgcc
     2881 gaaggtcagg tcggatgaaa tgttcaaccc ctcatccgac ccgcttcgcg ggccaccttc
     2941 tcccgcaagg ggagaagggt tccgtcgcct aggcccacgc cccgtacgga tccacgaacg
     3001 gcttgccgag cgcctcggcc acggccgggt gggtcagctc gcccttgagg acgttgaggc
     3061 ccttggccag gtgcctgtcg cgcttgagcg cgtcgaggcc gtggtcggcc agggccaggc
     3121 cgaacggcag ggtcgcgttg ccaagcgcct ccgacgaggt gcgcggggcg gcgccgggca
     3181 tgttggccac gcagtagtgg accacgccgt cgaccacata ggtcggctcg gcgtgggtcg
     3241 tcgggtggct ggtctcgaag cagccgccct ggtcgatcga gacgtcgacc agcacgctgc
     3301 cgggcttcat ccgcgacagg tgctcgcgct tgaccagctt gggcgccgcc gcgccggccg
     3361 tcagcaccgc gccgatgacc acgtcggcct tgaggatctc gtcctcgacc gcgtcgagcg
     3421 tcgagtagcg ggtcagcacc cggcccatga agatgtcgtc gagctcgcgc atgcgcggga
     3481 tcgaccgctc cagcaccacg acctcggcgt tgaggcccat ggccatgcgg gcggcgttca
     3541 tgccgaccac gcccccgccg agcaccgcca ctcgcgccgg cgacacgccg ggcacgccgc
     3601 agagcaggag gcccatgccg ccattgtgct tgagcagggt ttcggcggcc gagaagacgg
     3661 cgatgcggcc ggccacttcc gacatgggcg ccagcagggg caggccgccg cgggcgtcgg
     3721 tgaccgtctc gtaggcgatc gccgcgcagc cggacgccag cagtccctcg gtctgggcgg
     3781 ggtcgggcgc cagatgcagg taggtgaaca ggatgtggcg cggctcgagc cgctcccact
     3841 cgaccttttg cggctccttg accttcacga tcatgtcggc gccggagaac accgcgtcgg
     3901 cgtcggcgac gatggtcgcg ccggccttgc ggaaggcgtc gtcggcgtag ccggcgccct
     3961 ggccggcgcc ggcctcgacg aggacctgat ggccgtgggc cacgtactcg cgaacagccg
     4021 tgggggtcag acccacccga tgctcgccag gcttgatctc tgccggaact ccaacgcgca
     4081 tggtcatttc cttcccaacg gatttggcgc ggcaaatgtc ctgcgaatga cgtgaggtca
     4141 agcacgatct tgccaacgct cgcctgacgc gggagccgac tcgtgtgcgg attgacccgc
     4201 tggcaggctt gggctcacat cgttctgcag aaacactcag gcccgggaag ggctcccatg
     4261 acgccgtccg acgaaacccc tcacgatccc gcgcccgaat cggcgcccga cgcgccgctg
     4321 ctggtgatgc cgagcgacca tgtgatcgac gacgtcgccg ccttccacgc cgcgatcgcg
     4381 gc
//
LOCUS       DAORNX010000248         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14982_length_4367_cov_8.996985, whole genome shotgun sequence.
ACCESSION   DAORNX010000248 DAORNX010000000
VERSION     DAORNX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14982_length_4367_cov_8.996985"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /gene="lpdA"
                     /locus_tag="PLO65_11030"
     CDS             complement(<1..1157)
                     /gene="lpdA"
                     /locus_tag="PLO65_11030"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRD28822.1"
                     /translation="MAQYDVVIIGGGPGGYNAAIRAGQLGLKVACVEGRETLGGTCLN
                     VGCMPSKALLHASELYAAATGGEFAKLGIEVKPKLNLAQMMAQKDESVTALTKGIEFL
                     FKKNKVEWIKGWGRLDGPGKVVVKAVDGAETTLEAKNIIVATGSEPTPLPGVAVDNKR
                     IVDSTGALSLGEVPGHLVVIGAGVIGLELGSVWRRLGAEVTVVEYLDRVCPGMDEETA
                     KTFQRSLTKQGFKFQLGQKVTGATASASGVEITMEPVKGGETQTLAANYVLVAIGRRP
                     YTEGLGLETVGVVPDKRGMISNDHFRTGVGGVWVIGDVTSGPMLAHKAEDEGVACVEL
                     IAGKAGHVNYDLIPGVIYTAPEVATVGKTEEELKAAGVAYKVGKFPFTANSR"
     gene            complement(1245..1676)
                     /locus_tag="PLO65_11035"
     CDS             complement(1245..1676)
                     /locus_tag="PLO65_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28823.1"
                     /translation="MADGALDGLGWIARAVGLFYVLGGLFALRAARTELFLDDAIARI
                     TLSKPVPDAADRLRVATMVAIATLTVVSGALLVLLLKAALYAMLANAVLQGAYLAWAR
                     SALPPTDGASALGRRRTSNAFVVWLVMTALATLLFERGVLT"
     gene            complement(1683..2093)
                     /locus_tag="PLO65_11040"
     CDS             complement(1683..2093)
                     /locus_tag="PLO65_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28824.1"
                     /translation="MKILRWVLGALVLAYAGVCGLMAGMNALHKLGKLTTVPAEMQRM
                     VPLWDATPWWQLTIWGAVVLLLLVSAWRLFTGGRALGVFVLAIAADVATWWFMHNLPA
                     YQTVFTRAELQYDYYIIGGMAAAAVLIWMTERGK"
     gene            complement(2354..2785)
                     /locus_tag="PLO65_11045"
     CDS             complement(2354..2785)
                     /locus_tag="PLO65_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28825.1"
                     /translation="MALVSVARYADLQEARIAASRLDSAGIMSLVPDANLGSTNFFLG
                     QAIGGYRLCVIDEDLQAARDVLTGCRTENPDALNWRDHPDAVTAAPSSIFWAVVDPTA
                     GGWAWGSLRRRFSLPAAIVLVLSLAMIVTLVALTALSGAGP"
     gene            complement(2862..3272)
                     /locus_tag="PLO65_11050"
     CDS             complement(2862..3272)
                     /locus_tag="PLO65_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD28826.1"
                     /translation="MTISLDSSAVIDLINNRSEHTRQRFATAMASDEPIVISSIVIHE
                     VMFGALISRRPEEQKGVLESLIHPFSIVPLTIQDAEAAARVRKALRQAGKIIGHMDIL
                     VAGQALGRGWTVVTSNARDFRRVEGLKVIDWSAA"
     gene            complement(3269..3571)
                     /locus_tag="PLO65_11055"
     CDS             complement(3269..3571)
                     /locus_tag="PLO65_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD28827.1"
                     /translation="MPGFEEEGLPWRADASGARAKLFTHGGSQAVRLPKAFRFEGTEV
                     SVRREGDKVILEPVKPRYPRSPEEWTAFWARLDAMRGDDLIELPEDPPPLDRDFGW"
     gene            complement(3628..>4367)
                     /gene="odhB"
                     /locus_tag="PLO65_11060"
     CDS             complement(3628..>4367)
                     /gene="odhB"
                     /locus_tag="PLO65_11060"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRD28828.1"
                     /translation="AAPAAPAAPRAIHEREDRVKMTRLRQTIARRLKEAQNNAAMLTT
                     FNEVDMSAVMALRNQYKDSFEKKHGVKLGFMSFFVKACVAALQAVPDVNAEIDGTDIV
                     YKNHYDIGIAVGTEKGLVVPVVRDAEAMGLADIEKAIGALGRKARDGHLAIEDLQGGT
                     FSITNGGIYGSLMSTPILNAPQSGILGMHAIKDRPMVVGGQVVVRPMMYLALSYDHRV
                     VDGQGAVTFLVKVKEAIEDPQRLLLDV"
BASE COUNT          690 a         1511 c         1462 g          704 t
ORIGIN      
        1 cggctgttgg cggtgaacgg gaacttgccg accttgtagg cgacgcccgc ggccttcagc
       61 tcctcctcgg tcttgccgac cgtggcgact tccggggcgg tgtagatgac gccggggatc
      121 aggtcgtagt tcacatggcc ggccttgccc gcgatcagct cgacgcaggc cacgccctcg
      181 tcctcggcct tgtgcgccag catcggaccg ctggtcacgt cgccgatgac ccagaccccg
      241 ccgacgccgg tccggaagtg gtcgttgctg atcatgccgc gcttgtcggg gacgacgcct
      301 acggtctcca ggccgaggcc ttcagtgtag ggacgccggc cgatggccac gagcacgtag
      361 ttggccgcca gggtctgggt ctcgccgccc ttgaccggct ccatggtgat ctcgacgccg
      421 ctggcgctgg ccgtcgcgcc ggtgaccttc tggcccagct ggaacttgaa gccctgcttg
      481 gtcaggctgc gctggaaggt cttggcggtt tcctcgtcca tccccgggca gacgcggtcg
      541 aggtattcga ccaccgtcac ctcggcgccg aggcggcgcc agacgctgcc gagctcgagg
      601 ccgatcaccc cggccccgat caccaccaga tggccgggga cctcgcccag cgacaaggcg
      661 ccggtcgagt cgacgatgcg cttgttgtcg accgcgacac cgggcaacgg ggtcggctcg
      721 gaccccgtgg cgacgatgat gttcttcgcc tcgagcgtgg tctcggcccc gtcgacggcc
      781 ttcacgacca ccttgccggg tccgtcgagg cggccccagc ccttgatcca ctcgaccttg
      841 ttcttcttga acaggaactc gatgcccttg gtcagggccg tcacgctctc gtccttctgg
      901 gccatcatct gggccaggtt gagcttcggc ttgacctcga tgccgagctt ggcgaactcg
      961 ccgcccgtcg ccgccgcata gagctcggag gcgtgcaaca gcgccttgct gggcatgcag
     1021 ccgacgttca ggcaggtgcc gccgagggtc tcgcgcccct cgacgcaggc caccttcagc
     1081 cccagctggc cggcccggat cgccgcgttg tagccgccgg ggccgccccc gatgatgacg
     1141 acgtcgtatt gggccatggg aaggagccgc ctgtctgctg tgtgcgcatg aaccatatgg
     1201 ggcgcgctcg ccccctgcgc gaggcggttt aaggcgccgg gcccctaggt caacacgccc
     1261 cgctcaaaaa gcagcgtcgc cagggcggtc atgaccagcc agacgacgaa ggcgttgctc
     1321 gtgcgccgtc gccccagggc tgacgcgccg tccgtcggcg gcagggctga gcgcgcccag
     1381 gcgaggtagg cgccctgcaa cacggcattc gccagcatgg cgtagagcgc cgccttgagc
     1441 aggaggacga gaagcgcgcc gctgaccacg gtcagggtcg cgatcgcgac catggtcgcg
     1501 acgcgcaggc gatcggcggc gtctggaacg ggcttcgaca gggtaatccg ggcgatcgcg
     1561 tcgtcgagga acagctccgt gcgcgccgcc cgaagggcga acagcccacc caggacgtag
     1621 aacaggccga cggcgcgcgc gatccagccg agaccatcga gcgcgccgtc ggccatgccg
     1681 gtctacttcc cccgttcggt catccagatc aggacggccg ccgcggccat ccccccgatg
     1741 atgtagtagt cgtactgcaa ttcggccctg gtgaagaccg tctggtaggc cggcaggttg
     1801 tgcatgaacc accaggtcgc gacgtccgcg gcgatggcca gcacgaacac gccgagcgcc
     1861 cggccgcccg tgaacagccg ccaggccgag accagcagca gcagcacgac cgcgccccag
     1921 atggtcagct gccaccaggg cgtcgcgtcc cacagcggaa ccatccgctg catctcggcc
     1981 gggaccgtgg tcagcttgcc gagtttgtgg agggcgttca tgcccgccat caggccgcac
     2041 acgccggcat aggccagcac gagcgcgcca agcacccaac gcagtatctt catgtcgctt
     2101 gctcccccga gcatcggccg ctgaaggacg gcccgcgggg ggaaggctgg acgcaagttt
     2161 acgccacgtc aaatgcgctc gcctgtcagc ctctgttgtc gcgggtcctg cgttgcgccc
     2221 ggattccctt gggccatgga cgcgcgacga cagaaaggcc ggatggaagc gtggcttcga
     2281 cccggccgct atgacgatcg cccgctgccc cgcggcggac cgtcgatgat cgggaggctg
     2341 tggtcagggt cgctcatggg cccgcgccgg acagggcggt cagggcgacc agagtcacga
     2401 tcatggccag gctcaggacc aggacgatcg cggccggcag gctgaaccgg cgcctgaggc
     2461 tgccccaggc ccagccgccg gccgtcgggt cgacgacggc ccagaagatc gacgacgggg
     2521 ccgccgtgac ggcgtcagga tgatcccgcc agttgagcgc gtccgggttc tccgtccgac
     2581 agcccgtcag gacgtcgcgg gcggcctgga ggtcttcatc gatcacgcac aggcgatagc
     2641 cgccgatggc ctggccgaga aagaagttgg tcgagcccag attggcgtcg gggacgagcg
     2701 acataatccc ggccgagtcg agccgactgg ccgcgatccg ggcttcctgc agatccgcgt
     2761 agcgggcgac cgaaacgagc gccatatgcc ctccgcctct gaaaccggag gatgacccga
     2821 gaaggacccc accgtccagc agcagccgga acggccagga ctcaggcggc ggaccagtcg
     2881 ataaccttga ggccttcgac ccggcgaaag tcgcgcgcat tggatgtgac cactgtccag
     2941 ccccgcccga gcgcctggcc ggcgaccagg atatccatgt ggccgatgat cttccccgcc
     3001 tgtcgcaagg ccttccgcac ccgcgcggcc gcctccgcgt cctggatcgt caggggcacg
     3061 attgagaagg gatggatcag ggattccaga acccccttct gctcctccgg gcggcggctg
     3121 atcagggccc cgaacatgac ttcgtggatc acgatgctcg atatgacgat gggctcatcc
     3181 gatgccatgg cggtcgcgaa gcgctggcgt gtgtgttcag atcgattgtt gatcaggtcg
     3241 atcacggcgc tgctatcgag actgatcgtc accagccgaa atcccggtcc agaggcggag
     3301 gatcctccgg cagttcgatc aggtcatcgc cccgcatggc gtcaagccgg gcccagaacg
     3361 ccgtccactc ctccggcgac ctgggatagc gcggcttcac cggttcaagg atgaccttgt
     3421 caccctcgcg gcgcacgctg acctcggttc cttcgaaccg gaaggccttg ggcagccgca
     3481 cggcctgact gcccccgtgc gtgaacagct tggcgcgggc gccggacgca tcggcgcgcc
     3541 agggaagtcc ctcttcctcg aaaccaggca tcgcgaacct cgtatctaca atctgtagat
     3601 acgcccgcga gccgtcgcca gcaaggatca gacgtccagc aacagccgtt gcgggtcctc
     3661 gatggcttcc ttgaccttga ccaggaaggt caccgcgccc tggccgtcga ccacgcggtg
     3721 gtcgtagctg agcgccaggt acatcatcgg ccggaccacg acctggccgc ccacgaccat
     3781 cggccggtcc ttgatcgcgt gcatgcccag gatgccggac tgcggcgcgt tgaggatcgg
     3841 ggtcgacatc agcgagccgt agatgccgcc gttggtgatc gagaaggtgc cgccctgcag
     3901 atcctcgatg gccaggtggc cgtcgcgggc cttcctgccg agcgcgccga tggccttttc
     3961 gatgtccgcc aggcccatgg cctcggcgtc gcgcaccacg ggaaccacca ggcccttctc
     4021 ggtgccgacc gcgatgccga tgtcgtagtg gttcttgtag acgatgtccg tgccgtcgat
     4081 ctcggcgttg acgtcgggca ccgcctgcag ggccgcgacg caggccttca cgaagaagct
     4141 catgaagccg agcttcacgc cgtgcttctt ctcgaagctg tccttgtact ggttgcgcag
     4201 ggccatgacc gcgctcatgt cgacctcgtt gaaggtcgtc agcatggcgg cgttgttctg
     4261 ggcttccttc agccggcgcg cgatggtctg gcgcaggcgg gtcatcttca cccggtcctc
     4321 gcgctcgtgg atcgcgcgcg gcgcggcggg ggccgccggg gcggccg
//
LOCUS       DAORNX010000249         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_14989_length_4366_cov_7.598701, whole genome shotgun sequence.
ACCESSION   DAORNX010000249 DAORNX010000000
VERSION     DAORNX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14989_length_4366_cov_7.598701"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PLO65_11065"
     CDS             complement(<1..141)
                     /locus_tag="PLO65_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD28829.1"
                     /translation="MTERIHHDGVLVVGAGLAGLTAAMAAAPRKALVLSPTPLNQGCS
                     SAW"
     gene            complement(138..338)
                     /locus_tag="PLO65_11070"
     CDS             complement(138..338)
                     /locus_tag="PLO65_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28830.1"
                     /translation="MIAISKLGVAYMVKATPPEVTKAWGSLLPMNARNVVEELIRHGC
                     SLQDAFDAMIVADPTRAALELR"
     gene            complement(338..1423)
                     /gene="nadA"
                     /locus_tag="PLO65_11075"
     CDS             complement(338..1423)
                     /gene="nadA"
                     /locus_tag="PLO65_11075"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRD28831.1"
                     /translation="MADGFAQFDFTPEVEAATAPLWEKVKSRVSPLEWRVHAPLIAEI
                     NRLKREKNAVILAHNYMTPEIFHGVGDYVGDSLGLAREAARSDARIIVQAGVHFMAET
                     SKVLCPDKTVLIPDMRAGCSLASSITGADVRLIKQRYPGLPVVTYVNTTADVKAETDI
                     CCTSANAVQVVEWAAKEWGVDRVILIPDEFLARNVAAQTAVKIIAWQGRCEVHERFNA
                     RDIADLRAAYPGAEVLAHPECPEEVLAASDFAGSTAAMIDYVVDRQPKQVVLLTECSM
                     ASNIAAEAPGTEFLGPCNLCPHMKRITLANIHEALVREQFEVTVEPAMAARARQAVQR
                     MIDLPPPATPARYDLVKARHHVDVELI"
     gene            complement(1630..4014)
                     /locus_tag="PLO65_11080"
     CDS             complement(1630..4014)
                     /locus_tag="PLO65_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRD28832.1"
                     /translation="MPASESARPDDDREPLFWRAIPLHQPPWPQALLVTAAALAVASG
                     ARLATLGFPAGWGVSSTYFPAFIVATLFAGPAWGWGSLAVATAVGISSLWNPLATPSQ
                     QGTLILFALSGSLTVMVASGLRTALVRLRREAVARGEAEAMLQLAQEAGGLGLWDWDL
                     EVGKARWSPGVFHNLGIAPRTDPPGYQDLLDRVHPDDVDRLRAANAEAIREGRYYQID
                     YRAVGEDGQERWLHARGQVHRDAAGRAVRIVGYNLDVTARYRAAEQLRESEARFRSLA
                     DSAPALLWISRLGGAREFVNSAYVAFLGGGYEEALAVDWRTRLHPDDLQRILREQVAG
                     ESSMQPFSLEARYRRADGAWRWLRSFSQPRIDPAGEFAGFGGIAFDVTEAKQVEADLQ
                     HINELLEDRVQQVLAERDLAQAALIQSQKLEALGQLTGGVAHDFNNLLTVIIGALDIV
                     QRHPEDTGRTARLGKAALDAAQRGERLTRQLLAFSRRQPLRPEVAAIDGLLRDSETLF
                     RGALGERFMFDMALNAGEATVSIDPAQFDAAVMNLLVNARDASPGGGAVLVSTAVVRR
                     PESDGLPAGEYVAISVQDTGEGMDEATRARVFEPFFSTKPVGKGTGLGLSQVYGFVRQ
                     SGGEVSIVSAPGGGTTITLLLPVSAVAASSDDAGAPASPRPLTSLNVLLVEDDPDVAV
                     LAEAMLRELGHDVRLAADPAQALELLARDPRFTLLLTDIVMPGDMSGLDLARAAATAR
                     PGLPVLLSSGYTGDVLTSAEEAPWPLLRKPYTLDGLTGAIHEAIEAVARRYVNG"
BASE COUNT          654 a         1519 c         1559 g          634 t
ORIGIN      
        1 ccaggccgag gagcagccct ggttcaacgg cgtcggcgac agcaccagcg ccttgcgcgg
       61 cgcggccgcc atggcggcgg tcaggccagc caggcccgcg ccgacaacca ggaccccgtc
      121 gtgatggata cgctcggtca tctcagctcc agggcggcgc gggtcgggtc ggcgacgatc
      181 atggcgtcga acgcgtcctg caggctgcag ccgtggcgga tcagttcctc cacgacattg
      241 cgggcgttca tcggcagcag gctgccccag gcctttgtca cttccggcgg ggtcgccttg
      301 accatgtagg cgacgccgag cttgctgatg gcgatcatca gatcaactcc acgtcgacgt
      361 gatggcgggc cttgaccagg tcgtaacgcg ccggcgtggc gggcgggggc aggtcgatca
      421 tccgctgaac ggcctgacgg gcgcgcgcgg ccatggccgg ctcgacggtg acctcgaact
      481 gctccctgac cagcgcctcg tggatgttgg ccagggtgat ccgcttcatg tgcgggcaca
      541 ggttgcaggg gccgaggaac tcggtccccg gcgcctcggc ggcgatgttg ctggccatcg
      601 agcattcggt cagcagcacg acctgcttcg gctggcggtc gacgacatag tcgatcatcg
      661 ccgccgtcga gcccgcgaag tcgctggcgg ccagcacctc ctccgggcat tccggatggg
      721 ccagcacctc ggcgccggga taggcggcgc gcaggtcggc gatgtcccgg gcgttgaacc
      781 gctcgtgcac ctcgcagcga ccctgccagg cgatgatctt caccgcggtc tgggccgcca
      841 cattgcgggc gaggaactcg tcggggatca ggatcacccg gtcgacgccc cattccttcg
      901 cggcccattc gaccacctgc acggcgttgg cgctggtgca gcagatgtcc gtctcggcct
      961 tcacgtcggc ggtggtgttg acgtatgtga ccaccggcag gcccggatag cgctgcttga
     1021 tcagccgcac gtcggcgccg gtgatcgagc tggccaggct gcagccggcg cgcatgtcgg
     1081 ggatcagcac ggtcttgtcg gggcacagca ccttgctggt ctcggccatg aagtgcacac
     1141 cggcctggac aatgatcctg gcgtccgagc gggccgcctc gcgggccagg ccgaggctgt
     1201 cgccgacata gtcgccgacg ccgtggaaga tttccggcgt catgtagttg tgggccagga
     1261 tgaccgcgtt cttctcgcgc ttgaggcggt tgatctcggc gatcaacggc gcgtggacgc
     1321 gccattcgag gggcgagacg cggctcttca ccttttccca cagcggggcg gtggcggctt
     1381 ccacctcggg ggtgaagtcg aactgggcga aaccgtcggc catctcgatc ctccttatgc
     1441 tcaaactgag catttcaggg gcctgtaaaa tgccggaagc ggacgaacca ccccggcggc
     1501 gcgcgactta tgctataagt gagcataaga ggcaagagag atatagaccc gcgatgcagg
     1561 aggcgcaacc gctatcggat ggccaacccc gggccttcct ccatcgagga ggaaaggagt
     1621 ccgtcgggcc tatccgttca cgtaccgcct ggccaccgcc tcaatggcct cgtggatcgc
     1681 cccggtcagg ccgtcgaggg tgtatggctt gcgcaacagc ggccagggcg cttcctcggc
     1741 cgaggtcagc acatcgccgg tatagcccga actgagcagg accggcaggc ctggccgggc
     1801 ggtcgcggcg gcccgggcca ggtccagtcc ggacatgtct ccgggcatga cgatgtcggt
     1861 cagcagcagg gtgaagcgcg ggtcccgcgc caggagctcc agcgcctggg cgggatcggc
     1921 ggccagccgg acgtcgtgcc cgagctcccg caacatggcc tcggcgagca ccgccacgtc
     1981 ggggtcgtcc tcgaccagca ggacgttcag actggtcagc ggtcgcggtg aggccggggc
     2041 gccggcgtcg tcgctcgacg ccgcgacggc ggacacgggc aacaacaggg tgatggtcgt
     2101 gccgccaccc ggggcgctga cgatcgacac ctcgccgccg ctctggcgca caaagccata
     2161 gacctgggac aggcccaggc cggtgccctt gccgaccggc ttggtgctga agaagggctc
     2221 gaagacgcgg gcccgcgtcg cctcgtccat cccctcgccg gtgtcctgga cgctgatggc
     2281 cacatactcg cccgcgggca gcccgtcgct ctccgggcga cgaaccacgg ccgtcgagac
     2341 aagcaccgcg ccgccgccgg gagacgcatc gcgggcgttg accagcaggt tcatcaccgc
     2401 cgcgtcgaac tgcgccggat cgatgctgac cgtcgcctcg cccgcgttca gggccatgtc
     2461 gaacatgaac cgctcgccga gcgcgccgcg gaacagggtc tcgctgtcgc gcaggaggcc
     2521 gtcgatggcc gccacctccg gccgcagcgg ctggcgacgg gagaaggcca gcagctggcg
     2581 ggtcagccgc tcgccgcgct gggccgcgtc gagcgccgcc ttgcccagcc gggcggtgcg
     2641 cccggtgtcc tccgggtggc gctggacgat gtccagggcg ccgatgatca ccgtcagcag
     2701 gttgttgaag tcgtgggcca cgccgccggt cagctggccg agggcctcga gcttctgcga
     2761 ctggatcagg gccgcctggg ccaggtcgcg ctcggccagc acctgctgga cgcggtcctc
     2821 gagcagttcg ttgatatgct gcaggtcggc ctcgacctgc ttggcctcgg tgacgtcaaa
     2881 ggcgatgccg ccgaagccgg cgaactcgcc ggccgggtcg attcgcggct gggagaagga
     2941 gcgcagccag cgccacgcgc cgtcggcccg gcgatagcgg gcctccagcg agaacggctg
     3001 catcgaactc tcgccggcca cctgttcccg caggatgcgc tgcaggtcat cggggtgcag
     3061 gcgcgtgcgc cagtcgacgg ccagggcctc ctcgtaaccg ccgcccagga aggccacata
     3121 ggcgctgttg acgaactccc gcgccccgcc gagccgcgaa atccacagca gcgccggggc
     3181 gctgtcggcg aggctgcgga agcgcgcctc gctctcgcgc agctgctcgg cggcgcggta
     3241 gcgggcggtg acgtccagat tgtagcccac gatgcggacg gcgcggcccg ccgcgtcgcg
     3301 atggacctgc ccccgggcgt gcagccaccg ctcctggcca tcctcgccga ccgcccggta
     3361 gtcgatctgg tagtaccgcc cctcgcggat cgcctcggcg ttggccgccc gcagccggtc
     3421 gacatcgtcg ggatggaccc ggtcgagcag gtcctgatag ccgggcgggt cggtgcgggg
     3481 tgcgatgccg aggttgtgga acacgccggg cgaccagcga gccttgccga cctccaggtc
     3541 ccagtcccac agcccgaggc cgcccgcttc ctgggccagc tgcagcatcg cctcggcctc
     3601 gccccgggcg accgcctcgc ggcgcagccg gaccagcgcc gtgcgaaggc cgctggccac
     3661 catcaccgtc aacgaccccg agagggcgaa caggatcagc gtgccttgct gcgacggcgt
     3721 ggccagcggg ttccacagcg aactgatccc cacggccgtg gccaccgcca gcgagcccca
     3781 gccccaggct ggtccggcga acagggtggc gacgatgaag gccggaaaat aggtcgagga
     3841 cacgccccag ccggcgggga atcccagcgt cgccagcctt gcccccgagg cgaccgccag
     3901 cgccgccgcg gtgaccagca gcgcctgcgg ccagggcggc tgatgcagcg gaatggcgcg
     3961 ccagaacagg ggttcccggt catcgtcggg gcgggcagac tcgctcgcgg gcatgcgtcc
     4021 tctctcaggc cgaggcgccc cgacgagccg cagcatcttg cctcgatcat gaacgcgctt
     4081 aactgttcgg cgcaacagcg ggatgatgcg gtcggggtcg ccctggcgtt catcgcgctt
     4141 tggaaaccgg gaccgggggc ctatgggatc gtctccggcg cgggacggcg tcgaggagca
     4201 gggcacggtc gaaccatgac ggaccggaag accgcgtgag aagtcccctg cgccatttcg
     4261 gccggcagct gctcgtcatg ctggccgccg cgggcctgtg cgcctgcgtg accgactacc
     4321 gccacgccgc ggggaccggc cggaccggcg gcgtagcggc ggtggc
//
LOCUS       DAORNX010000250         4343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15072_length_4343_cov_7.413013, whole genome shotgun sequence.
ACCESSION   DAORNX010000250 DAORNX010000000
VERSION     DAORNX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15072_length_4343_cov_7.413013"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLO65_11085"
     CDS             <1..694
                     /locus_tag="PLO65_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD28833.1"
                     /translation="AEPPGAVHDDLLDRLLAVRDAETGQPLSDAEVRDQCATMLFAGF
                     ETTSRLLFWASYLLALDGAEQARLRAEVRAFPPDRVTSLEDLQHWPRLKMSLLEALRL
                     YPPVPIIMRQVVEPVVLCGESLGKGCLVWISPWVMHRHTAWWDNPTAFMPERFAGKAQ
                     PWISEPAYLPFGAGPRICIGAAFALAEASIVLATVLERFEVSLESDRPVMPVGGVTTA
                     PDHEPWFRVEAS"
     gene            complement(729..1778)
                     /locus_tag="PLO65_11090"
     CDS             complement(729..1778)
                     /locus_tag="PLO65_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD28834.1"
                     /translation="MKTPRGFFKPLAIGAPAPLRELPVRVERMIHFVPAHLEKVRAKV
                     PEIAPTVDVILGNLEDAIPAEAKDAARAGFIEMANATDFAAMGVGLWTRINCLNSPWH
                     LDDVQDIVAGVGDRLDVMMVPKVEGAWDIHYMDQLLALLEARHGLKKPILLHAILETA
                     EGVNNVEAICAASPRMQGISLGPADLAASRQMKTTRVGGGHPFYKVLEDPRDDGAPRA
                     SAQQDIWHYTFARMVDACAAHGIKPFYGPFGDISDGEACEQQFRNAFLLGCAGAWSLH
                     PNQIAIAKRVFSPDPGEVAFAKRILEGMPDGTGVVMLDGKMQDDATWKQAKVMVDCAR
                     QIAAKDPEYAALYGL"
     gene            complement(1842..2858)
                     /locus_tag="PLO65_11095"
     CDS             complement(1842..2858)
                     /locus_tag="PLO65_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRD28835.1"
                     /translation="MQTDLEALGHIDKLVLFNIGKTQVTSGGLIVALLTIVAALVAAR
                     LVGLTLQRVRARATRGGAALYIVEKLSVYGLVVFGAIAGISTLGVDLSSLAVFGGALG
                     VGVGLGLQGVVKEFVSGIVLIFDESLRVGDYIELESGQRGVIHEIGARATRIRNNDSV
                     DILIPNSQLIQGTVTNWTLRGHTRRIRIPFRAAYGVDKEKVRDAVLEAAMAVPFTLPD
                     SGAFRNQVWLVGFGDSALNFELVVWPRLEAVKRPNAMQAAYTWAIDDALRKAGIEAPF
                     PQQDLRIRTLFGEEGEAALKTLGLEPAAQRAGRARRSRASSNDAAEDLLRPDEEPDSP
                     PRKP"
     gene            complement(2981..3202)
                     /locus_tag="PLO65_11100"
     CDS             complement(2981..3202)
                     /locus_tag="PLO65_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRD28836.1"
                     /translation="MAMETRDSNGALLADGDSVTLIKDLKVKGSGGVTLKRGTLVKKI
                     RLTGDPDEIEANVDKVKGLVLRTEFVKKA"
     gene            complement(3275..4024)
                     /locus_tag="PLO65_11105"
     CDS             complement(3275..4024)
                     /locus_tag="PLO65_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRD28837.1"
                     /translation="MAWGDPLKRGDKGADVIELQMRLAGFRGTLPDGDYGPGTELQVV
                     TFQRDVMAMTRPSGAADAATLAAIDAFGAAHPVDFRQLACRCGVCGGFGRGLFKGRYR
                     GEVKAEVWHRYEYPGIHRMLLWAYRAAMVYSAPKGWRLTVNSGYRCSEDNRQHGRSST
                     NHHGKAIDIDILDAPSKMIDRERCNALRGLLVEKANAQVGWSAANRKALEPADIAPTW
                     VHYDVRSYSSRYLADRYFVKTQAELDAAPVV"
     gene            complement(4087..>4343)
                     /locus_tag="PLO65_11110"
     CDS             complement(4087..>4343)
                     /locus_tag="PLO65_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD28838.1"
                     /translation="LTARRFSQLLEDAAAGAGIDPARVSPHVLRHAFATHLLEGGADL
                     RVVQTLLGHSDIATTQIYTHVAGERLAEVVRSKHPLARKP"
BASE COUNT          594 a         1514 c         1524 g          711 t
ORIGIN      
        1 cgccgagccc cccggcgccg tccacgacga cctgctcgac cggctgctgg ccgtacgcga
       61 cgcggagacc ggccagcccc tgtccgacgc cgaggtgcgc gaccagtgcg cgaccatgct
      121 gttcgccggc ttcgagacca cctcgcgcct gctgttctgg gccagctacc tgctggcgct
      181 cgacggcgcc gaacaggccc gcctgcgcgc cgaggtccgc gccttcccgc ccgatcgcgt
      241 gacgtcgctg gaggatctgc agcactggcc gcggctgaag atgtcgctgc tcgaggccct
      301 gcggctttac ccgccggtgc cgatcatcat gcgccaggtg gtcgagccgg tcgtgctgtg
      361 cggcgagagc ctgggcaagg gctgcctggt ctggatcagc ccgtgggtca tgcacaggca
      421 cacggcgtgg tgggacaatc cgaccgcctt catgcccgag cgcttcgcgg gcaaggccca
      481 gccgtggatc agcgagccgg cctacctgcc gttcggcgcc gggccgcgca tctgcatcgg
      541 cgcggcgttc gccctggccg aggcgtcgat cgtgctggcg acggtgctgg agcggttcga
      601 ggtttcgctg gagagcgacc gtccggtgat gcccgtcggc ggcgtgacca ccgcgccgga
      661 ccacgagccg tggttccggg tggaggcgag ctgaggggac acgtaccttt ggtacatgtc
      721 cccttctgtc acagcccgta gagcgccgcg tattccgggt ccttggcggc gatctgcctg
      781 gcgcagtcga ccatcacctt cgcctgcttc caggtggcgt cgtcctgcat cttgccgtcg
      841 agcatgacca cgccggtgcc gtcgggcatg ccctcgagga tgcgtttggc gaaggccacc
      901 tcgcccgggt cggggctgaa cacccgcttg gcgatggcga tctggttcgg atgcaggctc
      961 caggccccgg cgcagccgag caggaaggcg ttgcggaact gctgctcgca ggcctcgccg
     1021 tcggaaatgt cgccgaacgg gccgtagaac ggcttgatgc catgggcggc gcaggcgtcg
     1081 accatcctgg cgaaggtgta gtgccagatg tcctgctggg cgctcgcgcg tggagcgcca
     1141 tcatctcgcg ggtcttccag caccttgtag aacgggtggc cgccgccgac gcgggtggtc
     1201 ttcatctgcc ggctggcggc caggtcggcc gggcccaggc tgatgccctg catgcgcggg
     1261 ctggcggcgc agatggcctc gacgttgttg acgccttccg ccgtctcgag gatggcgtgc
     1321 agcaggatcg gcttcttcag cccgtgcctc gcctccagca gcgccagcag ctggtccatg
     1381 tagtggatgt cccaggcccc ctcgaccttg gggaccatca tcacgtcgag ccggtcgccg
     1441 acgccggcga cgatgtcctg cacgtcgtcc aggtgccagg gcgagttgag gcagttgatg
     1501 cgcgtccaca ggcccacgcc catggcggcg aagtcggtgg cgttggccat ctcgatgaag
     1561 cccgcgcgcg ccgcatcctt cgcctccgcc gggatcgcat cctcaaggtt gccgaggatc
     1621 acgtcgacgg tcggagcgat ttcgggaacc ttggcgcgga ccttttccag gtgggcgggg
     1681 acgaagtgga tcatccgctc gacccgcacc ggcagctcgc gcagcggcgc cggggcgccg
     1741 atggccagtg gcttgaaaaa cccgcgcggc gtcttcatgg cgtcatcccc gtccctgctt
     1801 ttcgcaattg cgagatgttt tgcggcggcg ccgcgaggcc gtcaaggctt gcggggaggg
     1861 ctgtccggct cctcgtccgg gcgcagaagg tcctcggcgg cgtcgttgct cgaggcgcgg
     1921 gagcggcgcg cccggccagc gcgctgcgcg gccggctcca gccccagggt cttcagcgcg
     1981 gcctcgccct cctcgccgaa cagcgtgcgg atgcgcaggt cctgctgcgg gaagggcgct
     2041 tcgatgccgg ccttgcgcag ggcgtcgtcg atcgcccagg tgtaggccgc ctgcatggcg
     2101 ttgggccgct tgaccgcctc gagcctgggc cacaccacga gttcgaagtt cagcgccgag
     2161 tcgccgaagc cgaccagcca gacctggttc cggaaggcgc cgctgtcggg cagggtgaag
     2221 ggcacggcca tggccgcctc cagcaccgcg tcccgcacct tctccttgtc gacgccatag
     2281 gccgcgcgga acgggatgcg gatgcgccgg gtgtggccgc gcagggtcca gttggtcacc
     2341 gtgccctgga tcagctggct gttggggatc aggatgtcga cgctgtcgtt gttgcggatg
     2401 cgggtcgcgc gggcgccgat ttcgtggatc acgccgcgct gaccgctttc cagctcgatg
     2461 tagtccccga cccgcaggga ctcgtcgaag atcagcacga tgccggagac gaactccttg
     2521 accaccccct gcaggccgag accgacgccg acgcccaggg cgccgccgaa caccgccagg
     2581 gaggacaggt cgacgccgag ggtcgagatc cccgcgatcg cgccgaacac gaccaggccg
     2641 tacaccgaca gcttctcgac gatgtagagg gccgccccgc cccgggtcgc cctggcccgc
     2701 acccgctgaa gcgtcagccc gaccagccgc gcggccacca gggcggcgac gatggtcaac
     2761 agcgccacga tcaggccgcc ggacgtcacc tgggtcttgc cgatgttgaa caacaccagc
     2821 ttgtcgatat ggccgagggc ttccaggtcg gtctgcacgc gcgctccctt cgggggccgt
     2881 accttgcggc gggttcccgg cgaaggccgg tgtccggggt cggctacggt atcgtgacca
     2941 tagcgcgccg gcccggcgtg ccggcaaggc cgttcagcct ctaggccttc ttgacgaact
     3001 cggtgcgcag cacgaggccc ttcaccttgt cgacgttcgc ctcgatctcg tcgggatcgc
     3061 cggtcaggcg gatcttcttg accagggtcc cgcgcttgag ggtcacgccg cccgagccct
     3121 tgaccttgag atccttgatc agggtgacgc tgtcgccgtc ggcaaggagg gcgccgttgc
     3181 tgtcgcgggt ttccatggcc aggtcctttc gactcggaaa agcctgtcct tagacccccg
     3241 acccgcgccg gccaaactca agctgggccg ccccctacac caccggcgcc gcgtcgagct
     3301 ccgcctgcgt cttcacgaaa tagcggtcgg ccagatagcg gctgctgtag ctgcgcacgt
     3361 cgtagtgcac ccaggtcggg gcgatgtccg cgggctccag cgccttgcgg ttggcggcgg
     3421 accagccgac ctgggcgttg gccttctcca ccagcaggcc gcgcagggcg ttgcagcgct
     3481 cgcggtcgat catcttcgac ggcgcgtcga ggatgtcgat atcgatggcc ttgccgtgat
     3541 ggttggtcga ggaccggcca tgctggcggt tgtcctccga gcagcggtag ccggagttga
     3601 ccgtcagcct ccagcccttc ggcgcgctgt agaccatcgc cgcgcgatag gcccacagca
     3661 gcatccgatg gatgcccgga tactcgtagc ggtgccagac ctcggccttc acctcgcccc
     3721 tgtaccggcc cttgaacagg ccacgtccga agccgccgca gacgccgcac cggcaggcca
     3781 gttgccggaa gtcgacggga tgggcggcgc cgaaggcgtc gatggccgcg agggtggcgg
     3841 cgtcggccgc gccggagggc cgggtcatcg ccatcacgtc gcgctggaag gtcaccacct
     3901 gcagctccgt gcccgggccg tagtcgccat ccggcagggt tccgcgaaag cccgccagcc
     3961 gcatctgcag ctcgatcacg tcggcgccct tgtcgccgcg cttcagggga tcgccccagg
     4021 ccatgccggc ctccctcgcc cgttgagcga acgacgatac ctcaaccttt gcggctgtaa
     4081 aggatgctag ggcttgcgcg cgagcgggtg cttgctccgc accacctcgg ccagccgctc
     4141 gccggcgacg tgggtgtaga tctgggtggt ggcgatgtcg ctgtgcccga gcagggtctg
     4201 gaccacgcgc aggtcggcgc cgccctcgag caggtgggtg gcgaaggcgt ggcgcagcac
     4261 gtgcgggctg acccgcgccg ggtcgatgcc ggccccggcg gcggcatcct ccagcagctg
     4321 cgagaaccgc cgggcggtca gcc
//
LOCUS       DAORNX010000251         4337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15102_length_4337_cov_8.616067, whole genome shotgun sequence.
ACCESSION   DAORNX010000251 DAORNX010000000
VERSION     DAORNX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15102_length_4337_cov_8.616067"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLO65_11115"
     CDS             complement(<1..313)
                     /locus_tag="PLO65_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRD28839.1"
                     /translation="MAHGAVKHDYHLVNPSPWPLVGSFAAMVMMIGAVIWMKGLFGID
                     KGTPWIALLGLGLVLLTMVGWWIDVTREANAGDHTPVVSIGLRYGMILFIASEVMFFV
                     AW"
     gene            complement(372..971)
                     /locus_tag="PLO65_11120"
     CDS             complement(372..971)
                     /locus_tag="PLO65_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518887.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRD28840.1"
                     /translation="MRSPTSDADRRRAGRNARLAIICGVVFAGMVGAAYASVPLYKAF
                     CQLTGFDGTVRKAEAAPDRVLDRKLTIRFDANVRDLPWTFTPSQVSQELKIGDTGLAF
                     YKVTNNGKTPLTGRASYNVIPESAGLYFQKLECFCFTDQTIAPGATVEFPVVYFVDPK
                     FADDFETRGAQEVTLSYTFFPSTEKKTQAALGGSAGARL"
     gene            complement(952..1116)
                     /locus_tag="PLO65_11125"
     CDS             complement(952..1116)
                     /locus_tag="PLO65_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28841.1"
                     /translation="MTPEPIERPKYDVEAFRKARAGRNIALGLGLVAFVIIVFVVTVI
                     RMGANAQPHF"
     gene            complement(1113..2108)
                     /gene="cyoE"
                     /locus_tag="PLO65_11130"
     CDS             complement(1113..2108)
                     /gene="cyoE"
                     /locus_tag="PLO65_11130"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRD28842.1"
                     /translation="MKPSPMTQPAALKARAAVPALWTDYLQLLKPRVMSLVVFTALTG
                     LICAPTAINPVLGAVAVLCIAVGAGASGALNMWFDADIDARMRRTRGRPVPAGKVQGG
                     EALALGVALSLFSVMLMGMTTNWLAAGMLAFTILFYAVIYTMWLKRSTAQNIVIGGLA
                     GALPPAIGWAAASGDAPLNAWLLVLIIFMWTPPHFWALSLYTAEDYARAGVPMMPVVK
                     GAASTRRQILLYSLALIPVCLAPAATGLGGPVYLAVAALGGLVFLLLAWRLFRSKAGD
                     APDERIEGEDRLYAVRAGARDARNLFAFSILYLALLFAALLGEHLLGVAPLELLS"
     gene            complement(2174..3826)
                     /gene="ctaD"
                     /locus_tag="PLO65_11135"
     CDS             complement(2174..3826)
                     /gene="ctaD"
                     /locus_tag="PLO65_11135"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRD28843.1"
                     /translation="MAHAAAHDDHEAEHIPGFFTRWFFSTNHKDIGTLYIIFAIMAGL
                     VGGALSGLIRWELAEPGIQIFGPNSAVSLLGLIDQSHHGYNAVVTAHALIMIFFMVMP
                     AMIGGFGNWFVPIMIGAPDMAFPRMNNISFWLLVAAWVLLLTSMFVDGGPGHGFGGGW
                     TIYPPLSTSGHTGPSMDLAIFSLHLAGASSILGAINFITTIFNMRAPGMTLHRMPLFV
                     WSILVTVFLLLLSLPVLAGAITMLLTDRNFGTHFFDPAGGGDPVMFQHLFWFFGHPEV
                     YILILPGFGMISHIVSTFSKKPVFGYLAMAYAMVAIGFVGFIVWAHHMYTVGMGINLR
                     AYFVAATMVIAVPTGVKIFSWIATMWGGSLDFKTPMLWAMGFIFLFTVGGVTGVVLAN
                     AGIDYSLHDTYYVVAHFHYVLSLGAVFAIFAGFFYWFEKMWGVKYNEFLGCVQFWIMF
                     IGVNLVFFPQHFLGLQGMPRRYVDYPDAFSLWNKVSSIGYVITAVGVVVFLVILLEAA
                     IRRRKAEANPWGEGATTLEWTLSSPPPFHQFNEPPVIEADHH"
     gene            complement(3844..>4337)
                     /locus_tag="PLO65_11140"
     CDS             complement(3844..>4337)
                     /locus_tag="PLO65_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRD28844.1"
                     /translation="YEYPDQKIPEYISAMLPEDELAKRGLPHSLYRLAATEPMVVPVG
                     KVVRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFTAEKVGTYYGQCSELCGVDHA
                     FMPIEIKVVSQADFDAWVASKAPAAAPTPVAAPATDGAAPALTPTAAPAVPVAGVAPA
                     TAN"
BASE COUNT          802 a         1363 c         1544 g          628 t
ORIGIN      
        1 accaggcgac gaagaacatc acttccgagg cgatgaacag gatcatgccg tagcgcaggc
       61 cgatcgagac gaccggggtg tgatccccgg cgttggcctc gcgggtcacg tcgatccacc
      121 agccgaccat ggtcagcagg accaggccga gacccagcag cgcgatccag ggcgtgccct
      181 tgtcgatgcc gaacaggccc ttcatccaga tgaccgcgcc gatcatcatc accatcgcgg
      241 cgaaggatcc gaccagcggc caggggctgg gattgaccag atggtagtcg tgtttgacgg
      301 cgccgtgggc catgatcgtg cctgaacctc ttcttccccc ggaagcggcg ggccgctccc
      361 tcttcagggt gctatagcct cgccccggcg cttccgccaa gagccgcttg ggtcttcttc
      421 tccgttgagg ggaagaaggt gtaggaaaga gtgacttcct gggccccgcg ggtctcgaaa
      481 tcatccgcga acttggggtc cacgaaatag accacgggga attcgacggt cgccccggga
      541 gcgatggtct ggtcggtgaa gcagaagcac tcgagcttct ggaaatacag cccggccgat
      601 tcggggatca cgttgtagct ggcccggccc gtcagcggcg tcttgccgtt gttggtcacc
      661 ttgtagaagg ccagtcccgt gtcgccgatc ttcagctcct ggctgacctg gctgggcgtg
      721 aaggtccagg gcaggtcgcg gacgttggcg tcgaaccgga tggtcagctt gcggtcgagc
      781 acccggtccg gcgcggcctc ggccttgcgc acggtgccgt cgaagccggt cagctggcag
      841 aaggccttgt agagcggcac ggaggcgtag gccgccccga ccatgccggc gaacacgaca
      901 ccgcagatga tggccaggcg ggcgttgcgg cccgccctgc ggcgatcggc gtcagaagtg
      961 gggctgcgca ttggctccca tccggatgac ggtgacgacg aacacgatga tcacgaaggc
     1021 cacaaggccc aggcccaggg cgatgttgcg gccggcgcgc gccttgcgga acgcctcgac
     1081 gtcgtatttc ggccgttcga tcggctcggg cgtcatgaca gcagctccag tggcgcgacg
     1141 cccagcaggt gctcgcccag cagggcggcg aacagcaggg cgagatacag gatcgagaag
     1201 gcgaacaggt tgcgggcgtc ccgcgccccg gcgcggacgg cgtacagccg gtcctcgccc
     1261 tcgattcgct cgtctggcgc gtcgccggcc ttcgaccgga acagccgcca ggccagcagc
     1321 aggaagacca ggccgccgag cgccgccacc gccagataga ccggcccgcc caggcccgtc
     1381 gcggccgggg cgagacagac cgggatcagg gccagcgaat agaggaggat ctgccgccgg
     1441 gtcgaggccg cgcccttgac caccggcatc atcggaacgc cggccctagc gtagtcctcc
     1501 gcggtgtaga gggacagggc ccagaagtgc ggcggcgtcc acatgaagat gatcagcacc
     1561 agcagccagg cgttcagcgg cgcgtcgccc gaggcggcgg cccagccgat ggccggcggc
     1621 agggcgccgg cgagaccgcc gatgacgatg ttctgcgccg tcgagcgctt gagccacatc
     1681 gtgtagatca cggcgtagaa caggatggtg aaggccagca tgccggccgc cagccagttg
     1741 gtcgtcatgc ccatcagcat gaccgagaac agcgacaggg ccacgcccag ggccagggcc
     1801 tcgccgccct gcaccttgcc ggccggaacc ggccggccgc gggtgcggcg catgcgcgcg
     1861 tcgatgtcgg cgtcgaacca catgttcagc gcgcccgacg cgccggcccc gacggcgatg
     1921 cagagcaccg ccacggcgcc gagcaccggg tttatcgccg tcggcgcgca gatcagtccg
     1981 gtcagggcgg tgaacaccac cagcgacatc acccgcggct tgagaagctg caggtagtcc
     2041 gtccacagcg ccgggacggc ggcgcgggcc ttgagggcgg cgggctgggt catgggtgat
     2101 ggcttcataa gggaagggcg cgcccgggtc caaccctgac gcgcccctcc aggtgcttcc
     2161 gatggacgcg gacctagtgg tggtccgcct cgatcaccgg cggttcgttg aactggtgga
     2221 acggcggcgg cgaggacagg gtccactcca gcgtcgtggc gccttcgccc cagggattgg
     2281 cctcggcctt gcggcgacgg atggccgcct ccagcaggat gaccaggaac acgaccacgc
     2341 cgacggcggt gatcacgtag ccgatcgagg agaccttgtt ccacagcgag aaggcgtcgg
     2401 gatagtcaac gtagcggcgc ggcatgccct gcagcccgag gaagtgctgc gggaagaaca
     2461 ccaggttcac accgatgaac atgatccaga actgcacgca gcccaggaac tcgttgtact
     2521 tcacgcccca catcttctcg aaccagtaga agaagccggc gaagatggcg aacaccgcgc
     2581 ccaggctgag cacgtagtgg aagtgggcca ccacgtagta ggtgtcatgc aggctgtagt
     2641 cgatgccggc gttggccagc accacgccgg tgacgccgcc gacggtgaac aggaagatga
     2701 agcccatcgc ccagagcatc ggcgtcttga agtcgagcga gccgccccac atcgtcgcga
     2761 tccagctgaa gatcttcacc ccggtcggga cggcgatgac catcgtggcg gccacgaaat
     2821 aggcccgcag gttgatgccc atgcccacgg tgtacatgtg gtgggcccag acgatgaagc
     2881 cgacgaagcc gatggcgacc atggcgtagg ccatggcgag gtagccgaac accggcttct
     2941 tggagaaggt cgagacgatg tggctgatca tgccgaagcc cggcaggatc aggatgtaca
     3001 cttccgggtg accgaagaac cagaacaggt gctggaacat caccggatcg ccgccgccgg
     3061 cggggtcgaa gaagtgggtg ccgaagttgc ggtcggtcag cagcatggtg atggcgcccg
     3121 ccagaaccgg cagcgacagc agcagcagga agacggtgac caggatcgac cagacgaaca
     3181 gcggcatgcg gtgcagggtc atgcccggcg cgcgcatgtt gaagatggtg gtgatgaagt
     3241 tgatcgcgcc gaggatcgag ctggcgcccg ccaggtggag cgagaagatg gccaggtcca
     3301 tcgacgggcc ggtatgtccg gaggtcgaca gcggcgggta gatcgtccag ccgccaccga
     3361 agccgtggcc cggaccgccg tcgacgaaca tcgaggtcag cagcaggacc caggcggcga
     3421 ccagcagcca gaacgagatg ttgttcatcc gcgggaaggc catgtccggc gcgccgatca
     3481 tgatcggcac gaaccagttg ccgaagccgc cgatcatcgc cggcatgacc atgaagaaga
     3541 tcatgatcag ggcgtgggcg gtgaccacgg cgttgtagcc gtggtggctc tggtcgatca
     3601 ggcccagcag gctgacggcc gagttggggc cgaagatctg gatgccgggt tcagccagct
     3661 cccagcggat gagacccgac agggcgccgc ccaccagtcc cgccatgatg gcgaagatga
     3721 tgtacagcgt gccgatgtcc ttgtggttcg tcgagaagaa ccagcgggtg aagaagccgg
     3781 ggatgtgctc ggcttcgtga tcgtcgtgag cggcggcgtg agccatcggt ctgtatcctg
     3841 aaatcagttc gcggtcgccg gcgcgacgcc ggcgacgggg acggccggag ccgccgtggg
     3901 ggtcagggcg ggtgcggcgc catcggtcgc cggcgcggcg acgggtgtcg gcgcggcggc
     3961 gggagccttg gaagccaccc aggcgtcgaa gtcggcctgg ctgaccacct tgatctcgat
     4021 gggcatgaag gcgtggtcga cgccgcagag ctccgagcac tggccgtagt aggtgcccac
     4081 cttctcggcc gtgaaccagg tctcgttcag gcgtcccgga atggcgtcgg tcttgagacc
     4141 gaaggccggc agggcgaagg cgtggatcac gtcggcgccg gtgacctgga cccggacgac
     4201 cttgccgacc ggcacgacca tcggttccgt cgcggccagg cgatacaggc tgtgcggcag
     4261 gccgcgcttg gccagctcgt cctcgggaag catggccgag atgtactcgg ggatcttctg
     4321 gtcagggtac tcgtaac
//
LOCUS       DAORNX010000252         4305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15235_length_4305_cov_6.123059, whole genome shotgun sequence.
ACCESSION   DAORNX010000252 DAORNX010000000
VERSION     DAORNX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15235_length_4305_cov_6.123059"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..553
                     /locus_tag="PLO65_11145"
     CDS             83..553
                     /locus_tag="PLO65_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD28845.1"
                     /translation="MPLPEIYAAHRVVPAKGFADLKVGDRYPLPSRTLGDGNFVAFQA
                     VSLDNHPIHYDAEYCQALGHPGPLAHGLQVLCFTAAGAGLFPHEIGDALLGMIEVHAK
                     VLKSAYPGDTLYPALEITALTPQRTTGIVRMKATVTNQKGEVVLDGEHVYLLKL"
     gene            577..1848
                     /locus_tag="PLO65_11150"
     CDS             577..1848
                     /locus_tag="PLO65_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRD28846.1"
                     /translation="MTVPPPRDRRPSLLGRLGPGLISGAADDDPSGVATYSQAGAAFG
                     FNMLWNVVLAYPLLAGIQQIVARIGRVTGHGLAANIKRVFPAWMLALLVLAVLAANTI
                     NIAADIAAMGEAAALVAPGVDKFVYVAVFGLGCVLIEVFMSYKRYVKVLKWLTLSLLA
                     YAGIVFTVKIPWAEVAWRTLVPELALNPATLTMVVALFGTTISPYMFFWQSSQEVEDL
                     GHLPERRSLLGTPDLAASELRRIRLDTWAGMAFSNLIAFFIILTTAVTLHANGVTTID
                     TAAQAAQALRPIAGDLAFALFSLGIIGTGMLAVPVLAGAAAYAIAESFGWPMGLNRKL
                     GQAPGFYFIIAAATLGGVLMTFTRIDPIKALFWSAVINGLVAVPIMVVAMIIASRKSV
                     MGPFVIGRTLKILGWVATGVMAVAGVGMFLA"
     gene            1901..2701
                     /locus_tag="PLO65_11155"
     CDS             1901..2701
                     /locus_tag="PLO65_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28847.1"
                     /translation="MGADGRKCIFITGAASGIGLATAKRFAREGWFVGLADIDTAGLK
                     AAQVALGAGACSVHKLDVRDRAAWKKELAAFARATDGRLDVLLNNAGIANYGWFEDQP
                     DDEIDRQIDINIKGVSNGARAGLPLLIGTPGSTLVNVASCAGLFGAPKMAMYCATKFA
                     VKGLSEALDIEFSRHGVAVRCIMPWFVDTPILQAGSQGSNEKIADAIRAGGSEVYTVE
                     EAADVIWRSLQGKDVHWIVGKAGKQLRFATRFMPGSVRKQLKASMDQP"
     gene            complement(2664..>3122)
                     /locus_tag="PLO65_11160"
     CDS             complement(2664..>3122)
                     /locus_tag="PLO65_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRD28848.1"
                     /translation="RVAARALSGGVMTEVGNATHFHTIRVSPGWGPHLVRVGQVGLHV
                     FYRFGGRGGAPGAFDRTPARSGPEMGDRPIYASLGSPAEPIPYAALAPSTEAVFIAAT
                     AQAPTEITVSDKGIGGAGGKPSEPASAPRREAASEPVAVKAGPSTPSTAS"
     assembly_gap    3123..3157
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3158..3766)
                     /locus_tag="PLO65_11165"
     CDS             complement(<3158..3766)
                     /locus_tag="PLO65_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRD28849.1"
                     /translation="MSVALTGTLVGSACGAIVGGAYLMGGMAQAAVQHQRVSQIADVA
                     SGSYSDASLERMVDGMDPAALAVAQRHDPFTSAGGAQRDRQAALFAARLERRASAARP
                     SGEGLLLRASFGGPFNPAAEPFRLNGALEDSRDLECLTQAVYYEARGETPAGQAAVAQ
                     VVLNRARHPAFPKTVCGVVFQGAYRGGVCQFSFACDGSMHRRR"
BASE COUNT          652 a         1512 c         1454 g          652 t
ORIGIN      
        1 tccctactcc ctactcccta ctccctactc cctactccct actccctact ccctagcccc
       61 taatccccag cccctcagag ccatgcccct ccccgagatc tacgccgccc accgtgtcgt
      121 tcccgccaag gggttcgcgg acctgaaggt cggcgaccgt taccccctgc cgtcccgcac
      181 cctgggcgac ggcaatttcg tcgccttcca ggcggtcagc ctggacaacc atccgatcca
      241 ctatgacgcc gaatactgcc aggcgctggg ccatccggga ccgctcgccc acgggctgca
      301 ggtattgtgc ttcaccgccg ccggcgccgg cctgttcccg cacgagatcg gcgacgccct
      361 gctggggatg atcgaggttc acgcgaaggt cctcaagagc gcctatccgg gcgacaccct
      421 ctatcccgct ctggagatca ccgcgctgac gccgcaacgg acgaccggca tcgtccggat
      481 gaaagccacc gtcaccaacc agaagggcga ggtcgttctg gacggcgaac acgtctatct
      541 gctcaagctg taggccgaac ctcccccgag agacacatga ccgttccccc gccccgcgac
      601 cgcaggccca gcctgctcgg ccggctcggt ccgggcctga tcagcggcgc cgccgacgac
      661 gatccgagcg gcgtggccac ctattcccag gccggagccg cgttcggctt caacatgctg
      721 tggaacgtgg tgctggccta tccgctgctg gccggcatcc agcagatcgt cgcccgcatc
      781 ggcagggtca ccggccacgg cctggcggcc aacatcaagc gggtctttcc cgcctggatg
      841 ctggcgctgc tggtgctggc ggtgctggcg gccaacacga tcaatatcgc cgccgacatc
      901 gcggcgatgg gcgaagccgc cgccctggtg gcgccgggcg tggacaagtt cgtctacgtc
      961 gccgtgttcg ggctcggctg cgtgctgatc gaggtgttca tgagctacaa gcgctacgtc
     1021 aaggtgctca agtggctgac gctgtcgctt ctggcctacg ccgggatcgt gttcacggtg
     1081 aagatcccct gggccgaggt ggcctggcgc accctggttc cggaactggc cctgaacccg
     1141 gccaccctga ccatggtcgt ggcgctgttc ggcacgacca tcagccccta catgttcttc
     1201 tggcagagtt cgcaggaggt cgaggacctc ggccacctgc ccgagcgccg atcgctgctg
     1261 ggcacgcccg acctggccgc gtccgaactg cgccgcatcc ggctcgacac ctgggcgggc
     1321 atggccttct cgaacctgat cgccttcttc atcatcctga ccacggctgt gacgctgcac
     1381 gccaacggcg tgaccaccat cgacaccgcc gcccaggccg cccaggccct gcgacccatc
     1441 gccggggacc tggccttcgc gctgttcagc ctggggatca tcggcacggg aatgctggcg
     1501 gtgccggtgc tggcaggggc ggcggcctat gccatcgcgg agtcgttcgg ttggccgatg
     1561 ggcctgaacc gcaagctcgg ccaggcgccg ggcttctact tcatcatcgc cgcggccacc
     1621 ctcggcgggg tgttgatgac cttcacccgg atcgatccga tcaaggccct gttctggagc
     1681 gcggtgatca acggcctggt cgcggtgccg attatggtcg tggccatgat catcgcctcg
     1741 cgcaagtcgg tgatggggcc gttcgtcatc ggccggacgc tgaagatcct gggctgggtc
     1801 gccaccgggg tcatggccgt cgcgggcgtg ggcatgttcc tggcttgacc gccccggcgg
     1861 caaccggatt gtctggccga aagtcacggg gagaccgacc atgggcgccg acggccgcaa
     1921 gtgcatcttc atcaccggcg ccgccagcgg catcggcctg gccacggcga agcgcttcgc
     1981 ccgggagggg tggttcgtcg gcctggccga catcgacacc gccggcctga aggcggccca
     2041 ggtcgcgctc ggcgcgggcg cctgcagcgt ccacaagctc gatgtccgcg accgcgcggc
     2101 ctggaagaag gagctggccg ccttcgccag ggcgaccgac ggccggctgg acgtgctgct
     2161 caacaacgcc ggcatcgcca actacggctg gttcgaggac cagcccgacg acgaaatcga
     2221 ccggcagatc gacatcaata tcaagggcgt gtccaacggc gcccgggccg gtctgccgct
     2281 gctgatcggt acgcccggat cgacgctcgt caatgtcgcg tcctgcgccg gcctgttcgg
     2341 cgcgccgaag atggccatgt actgcgccac caagttcgcg gtgaagggtc tgtcggaggc
     2401 gctcgacatc gagttcagcc gccacggcgt ggcggtgcgc tgcatcatgc cctggttcgt
     2461 cgacacgccg atcctgcagg cgggctcgca gggcagcaac gagaagatcg ccgacgccat
     2521 ccgcgcgggc ggctcggagg tctacaccgt cgaggaggcc gccgacgtga tctggcgctc
     2581 gctccagggc aaggacgtgc actggatcgt cggcaaggcc ggcaagcagc tgcgtttcgc
     2641 cacccgcttc atgcccggtt cggtcaggaa gcagttgaag gcgtcgatgg accagccttg
     2701 acggcgaccg gctccgaagc cgcctcgcgg cgcggcgccg aggccggctc ggacggcttg
     2761 ccgccggccc cgccgatgcc cttgtccgag acggtgatct cggtcggggc ctgggccgtg
     2821 gcggcgatga acaccgcctc ggtcgagggc gccagggccg cgtaggggat cggttcggcg
     2881 ggcgagccga ggctggcgta gatcggacga tcgcccatct ccggacccga ccgcgccggg
     2941 gtgcgatcga aggcgcccgg cgcgccgccg cgaccgccga agcggtagaa cacatgcagc
     3001 ccgacctggc cgacccgcac cagatgcggt ccccagcccg gcgagacccg gatggtgtgg
     3061 aaatgggtgg cgttgccgac ctcggtcatc accccgcccg acagggcgcg ggcggcgacg
     3121 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg tcgacggtgc atcgagccgt
     3181 cgcaggcgaa gctgaactgg cagaccccgc cccggtaggc gccctggaag accacgccac
     3241 agaccgtctt ggggaaggcc ggatggcggg cccggttgag caccacctgg gcgacagcgg
     3301 cctgaccggc cggcgtctcg ccacgggcct cgtaatagac ggcctgggtc aggcattcga
     3361 gatcacggga atcctccagc gccccgttca ggcggaaggg ttcggcggcg gggttgaatg
     3421 gcccgccgaa gctggcccgc agcagcaggc cctcgcccga tgggcgggcg gcggaggcgc
     3481 ggcgctcgag ccgggcggcg aacagggccg cctgccggtc gcgctgggcg ccgccggcgc
     3541 tggtgaaggg gtcgtgacgc tgggccacgg cgagggccgc cggatccatg ccgtcgacca
     3601 tgcgttcaag ggaggcgtcg gaatagctgc ccgaggcgac gtcggcgatc tgcgacacgc
     3661 gctggtgctg gacggcggcc tgggccatgc cgcccataag gtaggcgccg ccgacgatcg
     3721 cgccgcaggc cgagcccacc agcgttccgg tcaaagcgac gctcatcagc gcgcgcacgg
     3781 gtgcgcgcgt ctggattcgg gatgacaaga gtttcgtgtc ctgtgcgacg agggcggatt
     3841 gccccccgac aatcaccctg aaagacgcca gcgcggcctg acaggatggg gacttaaccg
     3901 gttcacgaaa tggtcgaagt ccgacttgtg gccggtctta tggcccaaaa gcacgcgatt
     3961 cgcaagagaa tgttgcattg cagcatgaaa cactggccgg cggacccggc ccggctggcg
     4021 agtccccttg tttcacaacg actcccgggc gagtcgaaaa ggccggtgcg gcgccgcgaa
     4081 acggtcggca tcggcggcgg gaacctcgcc cttccccagc cgttcgaccc acaggccgcc
     4141 cgttcgggcg atccggagat atcgccatga gccttcgttc gacgcttccc ctcgccctcc
     4201 tggttctcgc gggcgcgggt ctggccggtt gcggccacaa catgcagcaa cgcgccgcca
     4261 ccggcgcgat cgccggcgcc gtcgtcgccg ggccgccggg cgccg
//
LOCUS       DAORNX010000253         4296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15280_length_4296_cov_6.976892, whole genome shotgun sequence.
ACCESSION   DAORNX010000253 DAORNX010000000
VERSION     DAORNX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15280_length_4296_cov_6.976892"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..755
                     /locus_tag="PLO65_11170"
     CDS             36..755
                     /locus_tag="PLO65_11170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRD28850.1"
                     /translation="MALTSPRARVWLDDELVGTASQAGYVFLGFDRDAGPTATVTVET
                     ELGATTYAMTIAPGEFDVQRIGGLPRDQVTPTDPALLERIRAEAAMKATGYASRIDGD
                     AFHNGFVMPLKTWRLSARFGGQRILNGVPQTPHFGSDLAAPQGSPIMAPAAGVVVLSN
                     PAMHYEGGLTMIDHGQGLISVYLHQSRIDVRAGDTVATGQVIGAVGMTGRATGPHLCW
                     RMKWRGRNLDPMLLVGLKPPA"
     gene            846..1340
                     /locus_tag="PLO65_11175"
     CDS             846..1340
                     /locus_tag="PLO65_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28851.1"
                     /translation="MDSGLGALKVLLVDDNQHMRAIVATILKGVGIREVREARDGAEG
                     LQALREWPADIAIIDFRMHPIDGVEFTRMVRNSADSKNPYLPIIMMTGFADRPRVCEA
                     RDAGVTELVVKPVTARSIIDRITAVVFHPRPFVRTEDYFGPRRRGIDDVNDGAAPGPA
                     QAQA"
     gene            complement(1467..2396)
                     /locus_tag="PLO65_11180"
     CDS             complement(1467..2396)
                     /locus_tag="PLO65_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD28852.1"
                     /translation="MADKRVTFGQDLVWRLEAIGFDLFTFLFRLLPVDAASWLGGALA
                     RLIGPLTRTHRLADQNLRLAFPEKDAAWRRRILAEQWECNGRTVAEFPMMDRLLPSTG
                     RVEVVNGERLAEIAAGGKPVVFVSGHFSNWEVMPAVIVNSGINCEITYRAANNPYVDR
                     RIRDSRFRYGVRLFAPKGGDGAREMLEALGKGVSVALMNDQKFNRGGVAAPFFGRPVM
                     AAPGPTRLALRFDTVLQPMCVQRTKGARFRVVVYEPIVLERTADRTADIEAGVRRVTA
                     FVEARVRERPGEWFWVHRRWPNEAYAELEATGG"
     gene            2567..2953
                     /locus_tag="PLO65_11185"
     CDS             2567..2953
                     /locus_tag="PLO65_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD28853.1"
                     /translation="MGNDIDVHLGKRLRRRRRLLGLTQQQLAGTVGVRFQQIQKYECG
                     ANRISASRLWQLSEALEVPVGYFYDGLSDADRRELASESNGEGGEMFARKETLDLIRA
                     YYQLGERPRRRLLDLAKSLNGETDAA"
     gene            3148..3960
                     /gene="hisN"
                     /locus_tag="PLO65_11190"
     CDS             3148..3960
                     /gene="hisN"
                     /locus_tag="PLO65_11190"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRD28854.1"
                     /translation="MTLSADRLAEFDAFLIELNRASAGVILPLFRADHGLENKLHGGF
                     DPVTEADKGAERAIRALIAHRYPDHGVIGEEYGEDRPDAEFVWVLDPVDGTRAFIAGL
                     PLWTTLIGLRHEGRPVLGSIGQPYIGELYIGGAATGSRLMSRGREAPLRVRACPKLTD
                     AIICTTDPDILTGPELGAWTQVRAAARLARLGLDAYGYAMVAAGTMDLALDTGLKVWD
                     VEAVIAVVEGAGGVCAHWDGSSAMSHGGKFAFAGDRACLDEALVALKRAARD"
     gene            complement(4031..>4296)
                     /locus_tag="PLO65_11195"
     CDS             complement(4031..>4296)
                     /locus_tag="PLO65_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28855.1"
                     /translation="WGWLDFAFSASAWLKRAPGVAAIKIPVLVLGAEEERLVDNADQR
                     EIVARIPNGRWLEVAGSYHEILQETDAIRAVFWREFDAVAAAL"
BASE COUNT          670 a         1526 c         1466 g          634 t
ORIGIN      
        1 tggtcaccgg acgctatcgc cagggcggct gcgccatcgc cctgaccagc ccccgggccc
       61 gcgtctggct cgacgacgaa ctggtcggga cagcctcgca ggccggctac gtgttcctcg
      121 gcttcgatcg cgacgccggc ccgacggcga ccgtgaccgt cgagaccgag ctgggcgcga
      181 ccacctacgc catgaccatc gcccccggcg agttcgacgt gcagcgcatc ggcggcctgc
      241 cgcgcgacca ggtcaccccg accgatccgg ccctgctcga gcggatccgc gccgaggcgg
      301 cgatgaaggc gaccggctac gccagccgca tcgacggcga cgccttccac aacggtttcg
      361 tcatgccgct caagacctgg cggctgagcg cgcgattcgg cggccagagg atcctcaacg
      421 gcgttccgca gacgccgcat ttcggatccg acctggccgc gccccagggg tcgccgatca
      481 tggctccggc cgccggggtg gtggtgctgt ccaaccccgc gatgcactac gagggcgggc
      541 tgacgatgat cgaccacggc cagggcctga tcagcgtcta cctgcaccag tcgcgcatcg
      601 atgtgcgggc cggcgacacg gtcgccaccg gccaggtcat cggcgccgtc gggatgaccg
      661 ggcgggccac cgggccgcac ctgtgctggc ggatgaagtg gcggggccgc aatctcgacc
      721 cgatgctgct ggtcgggctc aagccgccgg cctgatggta accaatctta agggtgcgga
      781 gccctattca aaccggaatc agaacaacgc gccggccatg aaccggcgca gggatcgagg
      841 gctccatgga ttcgggattg ggcgcgttga aagtgctgct ggttgacgac aaccagcaca
      901 tgcgcgccat cgtggccacg atcctcaagg gcgtgggcat ccgcgaggtc cgcgaagccc
      961 gcgacggcgc cgaggggctg caggccctgc gcgaatggcc cgccgacatc gccatcatcg
     1021 acttccgcat gcacccgatc gacggcgtcg agttcacccg catggtgcgc aattcggccg
     1081 acagcaagaa cccctatctg ccgatcatca tgatgaccgg cttcgccgac cgtccgcgcg
     1141 tctgcgaggc gcgcgacgcc ggcgtgaccg agctggtggt caagccggtg acggcccggt
     1201 cgatcatcga ccgcatcacc gccgtcgtct tccacccccg gccgttcgtc cgcaccgagg
     1261 actatttcgg cccgcgtcgg cgcggcatcg acgacgtcaa cgacggcgcc gccccgggtc
     1321 ccgcccaggc ccaggcctag gatcaatcga cagtcagccc ttgagggctt tgagaacccg
     1381 actggcgccg cgaaggcggg aacctccagg cttttcgtgg tcgcgcgttt tacccgatta
     1441 ccggttccca ccagtcggaa cgcgctctag ccgccggtcg cctcgagctc ggcataggcc
     1501 tcgttgggcc agcgccggtg cacccagaac cattcccccg gccgctcgcg cacgcgcgcc
     1561 tcgacgaagg cggtgacgcg ccgcacgccg gcctcgatgt cggccgtgcg gtcggccgtg
     1621 cgctcaagca cgataggctc gtagaccacc acccggaacc gcgcgccctt cgtgcgctgc
     1681 acgcacatcg gctgcagcac agtgtcgaag cgcagggcca ggcgggtcgg gccgggcgcg
     1741 gccatcaccg gccggccgaa gaagggcgcg gccaccccgc cgcggttgaa cttctggtcg
     1801 ttcatcaggg cgacggagac gcccttgcca agggcctcga gcatctcgcg cgcgccgtcg
     1861 ccgcccttgg gcgcgaacag ccgcacgcca tagcggaagc ggctgtcgcg aatgcgcctg
     1921 tcgacatagg ggttgttggc cgcgcgatag gtgatctcgc agttgatccc cgaattgacg
     1981 atcacggccg gcatcacctc ccagttggag aagtggcccg agacgaacac caccggcttg
     2041 ccgccggcgg cgatctcggc cagccgctcg ccgttcacca cctcgacccg gccggtcgag
     2101 ggcagcaggc ggtccatcat cgggaattcc gccaccgtgc ggccgttgca ttcccactgc
     2161 tcggcgagga tccgccggcg ccaggccgcg tccttttcgg gaaaggccag ccgcaggttc
     2221 tggtcggcca gcctgtgggt cctggtcagc ggcccgatca gccgggccag cgcgccgccc
     2281 agccacgatg ccgcatcgac aggcagcagg cggaacagga aggtgaacag atcgaagccg
     2341 atggcttcga gccgccagac caggtcctgt ccgaatgtaa cgcgcttgtc agccatgtcc
     2401 gttacctagc gtgcttcgag ccccggtggg aatcgcgccc gacgtgatct cccggcagga
     2461 gcttatccac agggccggat cgcttggcgc gcgacttgcg gaattgaagc ctgaccgttc
     2521 cgtttcggaa cagtcgtcct gaacaacaga acgggggcga cagcccatgg gcaacgacat
     2581 cgacgttcat ctcggcaagc ggcttcggcg ccgtcgccgt cttctcggcc tgacccagca
     2641 gcagctggcc ggcacggtcg gcgtgcgctt ccagcagatc cagaaatacg aatgcggcgc
     2701 caaccgcatc tccgcctcgc ggctgtggca gctgtccgag gccctcgagg tcccggtcgg
     2761 ctacttctac gacggcctgt ccgacgccga ccggcgcgag ctggccagcg agagcaacgg
     2821 cgaagggggc gagatgttcg cccgcaagga aaccctcgac ctgatccggg cctactacca
     2881 gctcggcgag cggccccgtc gccgcctgct cgacctggcc aagtcgctca acggcgagac
     2941 cgacgcggcc tgagattgcg tggttcgaca cgcgcttcgc gctgctcacc atgacgtgca
     3001 ggaatgtacg tcatcctggg ccggcccgac gggccgtgtc gaaggacgca ctccgcccga
     3061 gcccccgttg gggacagaca cctcagggtg tctgtccctt cggcggcggg tcttgttgag
     3121 ggacccgacc cccgctaacc gtggggcatg accctttccg ctgaccgtct ggccgagttc
     3181 gacgccttcc tgatcgagct gaaccgcgcg tcggccggcg tgatcctgcc gctgttccgg
     3241 gccgaccatg gcctggagaa caagctgcac ggcgggttcg acccggtcac cgaggccgac
     3301 aagggcgccg agcgggcgat ccgggcgctg atcgcgcacc gttatccaga ccacggggtg
     3361 atcggcgagg aatacggcga ggaccggccg gacgccgagt tcgtctgggt gctggatccc
     3421 gtcgacggca cgcgcgcctt catcgccggc ctgccgctgt ggaccaccct gatcggcctg
     3481 cgccacgagg gccggcccgt gctgggctcc atcggccagc cctatatcgg cgagctgtac
     3541 atcggcggcg ccgcgaccgg ctcgcggctg atgagccgcg gacgcgaggc gccgctgcgc
     3601 gtgcgcgcct gcccgaaact gaccgacgcg atcatctgca ccaccgatcc cgacatcctg
     3661 accggccccg agctcggcgc ctggacccag gtgcgggcgg ccgcgcgcct cgcccggctg
     3721 gggctggacg cctacggcta cgccatggtc gcggccggga ccatggacct ggcgctcgat
     3781 accggcctga aggtctggga cgtcgaggcg gtcatcgccg tggtcgaggg ggccggcggg
     3841 gtctgcgctc actgggacgg atcgtcggcg atgtcgcatg gcggcaagtt cgccttcgcg
     3901 ggcgatcggg cctgcctcga cgaggcgctg gtggcgctga aacgggcggc gcgggactag
     3961 cagagggaca tgcacttcag tgcgtgtccc cggcggcttc aggggacacg cactgaagtg
     4021 catgtccccg tcagagcgcg gccgcgaccg cgtcgaactc gcgccagaac accgcgcgga
     4081 tggcgtcggt ttcctggagg atctcgtggt agctgcccgc gacctcgagc cagcggccgt
     4141 tggggatgcg ggcgacgatc tcgcgctggt cggcgttgtc gacgaggcgt tcttcctcgg
     4201 cgcccagcac gagaaccggg atcttgatcg ccgccacgcc tggcgcgcgc ttgagccagg
     4261 ccgaggcgga gaaggcgaag tccagccagc cccagg
//
LOCUS       DAORNX010000254         4287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15320_length_4287_cov_5.795132, whole genome shotgun sequence.
ACCESSION   DAORNX010000254 DAORNX010000000
VERSION     DAORNX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15320_length_4287_cov_5.795132"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2102
                     /gene="ppsA"
                     /locus_tag="PLO65_11200"
     CDS             <1..2102
                     /gene="ppsA"
                     /locus_tag="PLO65_11200"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRD28856.1"
                     /translation="AAIAAAFDALRRERGPGLTVAVRSSATAEDLPTASFAGQHDSFL
                     HITDAASVVEACRRCFASLFTDRAIVYRINNGFDHLKVALSVAVMTMVNSDQAESGVA
                     FTLDTETGYRDVVFITGAYGLGENIVQGRVGPDEFYVHKPTYRQGYRAVLRRSLGAKE
                     TRLEHRRGDPSGALRSRPVPRRDRERFCLEDAAVLDLAGAAILIEDHFSRHAGAAVPM
                     DIEWGRDAGDGRLYILQARPETVSARRAAADYETYRLDGRAAVLTTGKAVGEKIAVGR
                     VRLIRGAADLKAFKPGEVLVAAATSPDWLPVMKAAAAIVTDQGGRTCHAAIVARELGV
                     PAVVGVGDATDRLVDGQTVTVSCAEGEVGRVYDGEVAFSVTRTALGELPRADTAIMVN
                     LAQPDLAFRTGLLPAAGVGLARLEFIIGEQIGIHPMAAAHPDQVSSARDRAIIARRSR
                     GETSPAEAFVGKLAEGVGVIAAAFHPRPVIVRLSDFKTNEYARLTGGRAFEPVEENPM
                     LGFRGAARYAHPRYADGFALECAALRRVRETLGLTNVKVMVPFCRRVEEAEQVLAAMA
                     DHGLKRGENGLEVYVMCEIPNNVLRIDAFAALFDGFSIGSNDLTQLVLGVDRDSETVA
                     FDFDERDPGVLEMLRMAVEGARRNHRPVGICGEAPATFPEIADFLVRLGIDSISVNPA
                     SLPATLEIVSRSESRRP"
     gene            2396..3118
                     /locus_tag="PLO65_11205"
     CDS             2396..3118
                     /locus_tag="PLO65_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD28857.1"
                     /translation="MTTPSGPRIVFEARGLTKVYRTGEVEVQALRGLDLDLYAGEFAV
                     FLGPSGSGKSTLFNILGGLDRATGGSARFEGLELTTLDERALTRYRRRNVGFVFQFYN
                     LVPSLTARENVALVTEIADNPMSPDEALEMVGLTDRAEHFPAQLSGGEQQRVAIARAI
                     AKRPEVLLCDEPTGALDLATGVRVLEALAFVNETLGATTAVITHNTSIREMAHRVLYF
                     GDGRVSRVEQNAARKAARDLSW"
     gene            3115..>4287
                     /locus_tag="PLO65_11210"
     CDS             3115..>4287
                     /locus_tag="PLO65_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD28858.1"
                     /translation="MTVLDRKLLRDIRRLWAQALAIALVMASGVATLAIGDGAYRSIH
                     ETRAAYYDRGGFADVFAQARRAPEGLAREIAALPGVAVAETRIVQGALLDLPDLAEPA
                     SAVLVSLPGGRQPRLNRLSLRSGRLPESAAIDEAVVSEAFARAHGFHAGDRFDAIMNG
                     RRRSLRIVGVALSPEYVYAIGPGDIVPDDRRFGVVWMPRDALEAAFDLDGAFNSVSLK
                     IWPGANEADVIRRLDDLLAPYGGAGAYGRRDQSSNSYLDSELQQLNAMRRVLPPIFLL
                     VSAYLINMTLSRLLSLEREQIGLFKAVGYADWPIALHYLKLALLISAIGVAIGLAAGA
                     WLGHGVTRLYAEFFHFPFLVFVQDGRTWILAIVTAGGAALLGAVRAVGAIVRLAPAT"
BASE COUNT          597 a         1537 c         1498 g          655 t
ORIGIN      
        1 cggcggcgat cgccgccgcc ttcgacgcgc tgcggcgcga gcggggcccg ggcctgacgg
       61 tggccgtgcg cagctccgcc acggccgagg acctgcccac ggccagcttc gccggacagc
      121 atgacagctt cctgcacatc accgacgcgg cctcggtcgt cgaggcctgc cgccgctgct
      181 tcgcctcgct gttcaccgac cgggccatcg tctaccggat caacaacggg ttcgatcacc
      241 tgaaggtcgc gctgtcggtg gcggtgatga caatggtcaa ttccgaccag gctgaaagcg
      301 gggtggcctt caccctcgac accgagaccg gctaccgcga cgtggtgttc atcaccggcg
      361 cctacggcct gggcgagaac atcgttcagg gccgtgtcgg acccgacgag ttctatgtcc
      421 acaaacctac ctaccggcag ggctaccgcg ccgtgctgcg ccgctcgctg ggcgccaagg
      481 agacccgcct ggaacatcgg cgcggtgatc cgtccggcgc gctgcggtcc cggcccgttc
      541 cgcgtcgtga ccgcgagcgg ttctgcctcg aggacgccgc cgtgctcgac ctggccgggg
      601 cggcgatcct gatcgaggat catttctccc gccatgcggg cgcggccgtg ccgatggaca
      661 tcgagtgggg gcgcgacgcc ggggacggcc ggctctacat cctgcaggcc cgcccggaga
      721 ccgtctctgc ccggcgagcc gccgccgact acgagaccta ccgccttgac ggccgggccg
      781 ccgtgctgac cacgggcaag gcggtcgggg agaagatcgc ggtcggccgg gtgcggctga
      841 tccgcggcgc cgccgacctg aaggccttca agccgggcga ggtgctggtc gccgccgcga
      901 ccagtcccga ctggctgccg gtgatgaagg cggccgccgc catcgtcacc gaccagggcg
      961 gccgaacctg ccacgcggcc atcgtcgccc gcgagctggg cgtgccggcg gtggttggcg
     1021 tcggcgatgc gaccgaccgg ctggtcgacg gccagacggt gaccgtcagc tgcgccgagg
     1081 gcgaggtcgg ccgcgtctat gatggcgaag tcgccttctc ggtcacgcgc acggcgctgg
     1141 gcgagctgcc acgcgccgat acggcgatca tggtcaatct ggcccagccc gatctcgcct
     1201 ttcgcaccgg cctgctgccc gccgccggcg tcggcctggc gcgactggag ttcatcatcg
     1261 gagagcagat cggcatccac ccgatggcgg cggcccatcc cgaccaggtc agctcggcgc
     1321 gggaccgggc gatcatcgcc aggcggtcgc ggggcgagac ctcgccggcc gaggccttcg
     1381 tgggcaaact ggccgagggc gtcggggtga tcgcggccgc cttccacccg cgaccggtca
     1441 tcgtgcggct gtccgacttc aagaccaatg agtacgcccg gctgaccggc ggccgcgcct
     1501 tcgagccggt ggaggaaaac ccgatgctcg ggttccgcgg cgcggcgcgc tacgcccacc
     1561 cgcgctacgc cgatggcttc gccctggagt gcgcggcgct caggcgggtg cgcgagaccc
     1621 tgggcctgac gaacgtgaag gtcatggtgc ccttctgccg ccgggtcgag gaggccgagc
     1681 aggtgctggc ggcgatggcc gaccatggcc tgaagcgcgg cgagaacggg ctggaggtct
     1741 acgtcatgtg cgagatcccc aacaacgtgc tgcggatcga cgccttcgcc gccctgttcg
     1801 acggcttctc gatcggctcc aacgacctga cccagctggt gctcggggtc gaccgggatt
     1861 ccgagaccgt ggccttcgac ttcgacgagc gcgatcccgg cgtgctggag atgctgcgga
     1921 tggccgtcga gggcgcgcgc cgcaatcatc gacccgtcgg catctgcggc gaggcgccgg
     1981 ccaccttccc cgagatcgcc gacttcctcg tccggctcgg catcgactcg atcagcgtca
     2041 accccgccag tctgcccgca acactggaga tcgtcagtcg cagcgaatcc cgaagacctt
     2101 gacccggtca atggcggccg ggacgcagca tgcatcctgt cacccgggcg gcgactctcc
     2161 ctcaggaagg cgacgcccgc ggagatgacc cccatctccg cgacgcttgg agatctggtg
     2221 cgcgaaatca tcccccctcc cccacgcgca ccgggcgggt gccccggcct ggacccgcgg
     2281 cgcccggtgt cgctccggcg acccgcgccg cgggcatccg tccacggccc ttgttcctga
     2341 tcaaatcgtc ccggcggccg aacgggcatt ctgcctctcc aggatggagc ggaccgtgac
     2401 gacaccgtcg ggacccagga tcgtcttcga ggcccggggc ctgacaaagg tctatcggac
     2461 cggggaggtc gaggtgcagg ccctgcgcgg cctcgacctg gatctctatg ccggcgagtt
     2521 cgccgtcttc ctgggaccgt ccggcagcgg caagtcgacc ctgttcaaca tcctcggcgg
     2581 gctcgaccgc gccaccggcg gctcggcccg gttcgagggg ctcgaactga ccacgctcga
     2641 tgaacgcgcg ctgacgcggt atcggcggcg caacgtcggc ttcgtcttcc agttctacaa
     2701 tctcgtgccg agcctgacgg cgcgggaaaa cgtcgccctc gtcaccgaga tcgccgacaa
     2761 cccgatgtcg ccggacgagg cgctcgagat ggtcggcctg accgatcgcg ccgagcattt
     2821 tcccgcccag ctgtcgggcg gcgaacagca gagggtggcc atcgcccggg ccatcgccaa
     2881 acgccccgag gtgctgctgt gcgacgagcc gaccggggcg ctcgatctgg ccaccggcgt
     2941 gcgcgtcctc gaggcgctgg ccttcgtcaa tgagacgctc ggcgcgacga cggcggtcat
     3001 cacccacaac acctcgatcc gcgaaatggc ccatcgcgtg ctctatttcg gcgacggccg
     3061 ggtcagccgg gtcgaacaga acgccgcccg gaaggcggcg cgggacctca gctggtgacc
     3121 gtgctcgacc gcaagctgct gcgcgacatc cgccgactgt gggcccaggc cctggcgatc
     3181 gccctggtga tggcctccgg cgtcgcgact ctggcgatcg gagacggcgc ctaccggtcg
     3241 atccatgaga cccgcgccgc ctactatgac cggggcggct tcgccgacgt gttcgcccag
     3301 gcgcgacgcg ccccggaggg actggcgcgg gagatcgccg ccctgcccgg cgtggcggtc
     3361 gccgagaccc gcatcgtcca gggcgccctg cttgacctgc ccgatctggc tgagccggcg
     3421 tcggcggtgc tggtctcgct gcccggcggc cgccagccgc ggctgaaccg gctcagcctg
     3481 cggtcgggtc gcctgcctga gtcagcggcg atcgacgagg cggtggtcag cgaggccttc
     3541 gcccgggcgc atggcttcca cgcaggtgac cgcttcgatg cgatcatgaa cgggcgcagg
     3601 cgatcgctca ggatcgtggg cgtcgccctg tcgcctgaat acgtctacgc catagggccg
     3661 ggcgacatcg tgcccgacga ccggcgattc ggtgtggtct ggatgccgcg tgacgcgctg
     3721 gaggcggcgt tcgacctcga tggcgccttc aacagcgtca gcctgaagat ctggcccggc
     3781 gccaacgagg ccgacgtgat ccggcggctc gacgacctgc tcgcgcccta cggcggcgcc
     3841 ggggcctatg gccgccggga ccagtcctcg aacagctatc tcgacagtga gctgcagcag
     3901 ctaaacgcca tgcggcgggt gctgccgccg atcttcctgc tggtgtcggc ctatctgatc
     3961 aacatgaccc tgtcgcggct gctgagcctg gagcgggagc agatcggact gttcaaggcg
     4021 gtcggctacg ccgactggcc gatcgccctg cattatctca agctggccct gctgatctcc
     4081 gccatcggcg tggcgatcgg gctggcggcc ggcgcctggc tcggtcacgg cgtgacccgg
     4141 ctctacgcgg agttcttcca cttcccgttc ctggtgttcg tgcaggacgg gcgaacctgg
     4201 atcctggcca tcgtcaccgc ggggggcgcg gccctgctgg gcgctgtgcg cgcggtcggg
     4261 gccatcgttc gcctggcgcc ggcgacc
//
LOCUS       DAORNX010000255         4256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15468_length_4256_cov_6.368960, whole genome shotgun sequence.
ACCESSION   DAORNX010000255 DAORNX010000000
VERSION     DAORNX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15468_length_4256_cov_6.368960"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLO65_11215"
     CDS             <1..875
                     /locus_tag="PLO65_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD28859.1"
                     /translation="SRTLIVPISASQDTAGPMTRTVRDAADMLTVMAGGDPADPATAE
                     ADAHRSDYAAGLRTDALKGVRIGVMRYAAGFHRETDAVFEQALAALRAQGAILVEISD
                     FPNRDKIGADELVVLLTELKAGLNAYLASTDPLKVRTRTLAEIIAFNRANADREMPLF
                     GQELFEQAEKTAGLADPAYLKARDEARRLAGPEGIDALLTKHDVAALVGPTLGPAWLI
                     DPVLKDRYVGGGAGGAAAVAGYPHLTVPMGQVGGLPVGLSFVGPKWSEARLLAYGFAF
                     EQATHARRPPAMGD"
     gene            complement(943..2433)
                     /locus_tag="PLO65_11220"
     CDS             complement(943..2433)
                     /locus_tag="PLO65_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28860.1"
                     /translation="MKTLTRFLTAAGAALIVAAAASPSEAFPDAAAGIDAQIRPNFGG
                     LITPPLRPRWRPDRWRPRGYRGYRPGRGGYYPPERVEMSLTVDCNAPAPYDSGYQAPN
                     GPSAGQGQYNDPGYGPDDPDGGQYAPTPVADALQRLADGGTLYISGTCRETVWIEHPV
                     TIAGRGLSIFDEGAPQVATIAPPEGASCIKVAPGVQGVELRDLVLTNDKGGRAACVEA
                     WDSKVALVRTTVRYWGDTAAVFASGGTLILQESFIDARTWDAAVVADAGVIKISRTRI
                     LAEETGVDLTPGVGQSLIDQTGILGRDAALPAGNGIMVRGQRSGSGALKVNNTVVCGW
                     RNGLHLDRGSIVEVNRSRFCRNTVGVVSDGDLTLLESAINGREVGVYAATGRATIKRN
                     RFYGWSSRPIWIEQGVVFDFEPNYAYFNDDCWKRSWEQGVYCQRSGGLPPTLRDESGF
                     TNPYRDWWEADAYDRGYMRDGPPAAQPYPTRPAPPAKPRWGSRRGS"
     gene            complement(2430..3902)
                     /locus_tag="PLO65_11225"
     CDS             complement(2430..3902)
                     /locus_tag="PLO65_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD28861.1"
                     /translation="MRDASDADGRPFGAAQGPLIRGASVAALVAAVVIPLVGCDTGAE
                     SFFGKPGDTACPRPQIPTGVAPQFNTQQLEIRTLRRRGFTGDFFSQLELARRYEGQRA
                     ADKNLEDPVEAAVWYAVALSNGDGYAPISAYGRRSPQGEQKAIARYDDCRAVERGIAY
                     RALDRLLSQMSSEEQEKVRSRVIYILSTEGAGGYRTLARMHDNFFGPFGEPSDNPQAA
                     AARGKPYKPGTPQVLNLFPRNDVDAYLYNYLAVQTGDVGAYVMLKDFERSSAQRAAAG
                     QYVEAKAKRWIPPYEFYPPESPSSGVPHSDESDQIGDAQQVALARVRELPFVHIGEAL
                     AYLRSTPRTVTNEDALYPQEVQDFQARIGRPQTGVLAPIEKVRIIQYAAVNGSPKAQL
                     VLAVMYAEGVGVPRDYARSFHWFAEADRQGSGEAKYAMSTYFSLGVAGVADQDKAKAV
                     VYQIDSALSGFKPSVGRLQQVLAQVSRSPRQAPRDRDYAR"
BASE COUNT          621 a         1482 c         1514 g          639 t
ORIGIN      
        1 tctcccggac cttgatcgtg ccgatcagcg ccagccagga caccgccggc ccgatgaccc
       61 gcacggtgcg cgacgccgcc gacatgctga cggtgatggc cggcggcgac ccggccgacc
      121 cggccacggc cgaggccgac gcccaccgga gcgactacgc cgccggactg cggaccgatg
      181 ccctgaaggg tgtgcgcatc ggggtcatgc gctacgccgc cggcttccat cgcgagacgg
      241 acgccgtctt cgaacaggcc ctggcggccc tcagggcgca gggtgcgatc ctcgtcgaga
      301 tttccgactt tcccaaccgc gacaagatcg gagcggacga actggtggtc ctgctgaccg
      361 aactgaaggc ggggctgaac gcctatctgg cctcgaccga cccgctgaag gtcaggaccc
      421 gcaccctggc ggagatcatc gccttcaacc gcgccaacgc cgaccgggaa atgccgctgt
      481 ttggccagga gctgtttgaa caggcggaga agaccgccgg cctcgccgat ccggcgtacc
      541 tcaaggcgcg cgacgaggcg cgccgtctgg ctggtccgga gggcatcgac gcgctgctga
      601 cgaaacatga cgtggccgcg ctcgtcggcc cgacgctggg cccggcctgg ctgatcgatc
      661 cggtgctcaa ggaccgctac gtcgggggcg gcgccggcgg cgcggcggcg gtggcgggct
      721 atccgcatct gaccgtgccg atgggccagg tcggtggtct gccggttggc ctgagcttcg
      781 tcggcccgaa atggagcgag gcgcggttgc tggcctatgg cttcgccttc gagcaggcga
      841 cccacgcgcg ccggccgccg gcgatgggcg actagcagcc gggtgctgcg ccggcgccct
      901 ccgcgggccg aaacgcccta cgccccctgc gccctgcgtc ccctagcttc cccggcggct
      961 gccccagcgg ggcttggccg gaggagccgg acgggtcgga tagggctggg cggctggcgg
     1021 accgtcacgc atatagccgc ggtcgtaggc gtcggcctcc caccagtcgc gataggggtt
     1081 ggtgaagccg ctttcgtcgc gcagggtcgg cggcaggccg ccgctgcgct ggcagtagac
     1141 gccctgctcc cagctgcgct tccagcagtc gtcgttgaaa taggcgtagt tgggctcgaa
     1201 atcgaacacc acgccctgtt cgatccagat cggccggctg ctccagccgt agaaccggtt
     1261 gcgcttgatg gtcgcccggc cggtggcggc gtagacgccg acctcgcggc cgttgatcgc
     1321 cgattccagc agggtcaggt cgccgtcgga gaccacgccc acggtgttgc ggcagaagcg
     1381 gctgcgattg acctcgacga tcgagccgcg atcgaggtgc aggccgttgc gccagccgca
     1441 gaccacggtg ttgttgacct tcagggcccc cgagccgctg cgctggccgc ggaccatgat
     1501 gccgttgccg gcgggcaggg ccgcgtcgcg gccaaggatg ccggtctggt cgatcaggct
     1561 ctggccgacg ccgggtgtca ggtcgacgcc ggtctcctcg gccaggatgc gcgtgcggga
     1621 gatcttgatg acccccgcgt cagcgaccac cgccgcgtcc caggtgcggg cgtcgatgaa
     1681 gctctcctgc aggatcaggg tgccgcccga ggcgaacacc gccgcggtgt ccccccagta
     1741 gcggaccgtc gtccgcacca gcgccacctt ggagtcccaa gcctcgacgc aggcggcgcg
     1801 cccacccttg tcgttggtca gcacgagatc gcgcaactcg acgccctgga cgccgggagc
     1861 gaccttgatg cacgaggcgc cttcgggcgg cgcaatggtc gccacctgcg gcgcgccctc
     1921 gtcaaagatc gacaggccgc ggccggcgat tgtcaccgga tgctcgatcc agacggtctc
     1981 gcgacaggtg ccgctgatgt acagcgtgcc gccgtcggcc agccgctgca gcgcgtcggc
     2041 gaccggcgtc ggcgcatact ggccgccgtc tggatcgtcg ggtccgtagc cggggtcgtt
     2101 gtactggccc tgccccgcgg aaggaccatt gggggcctgg tagccggaat cgtagggagc
     2161 cggggcgttg cagtcgaccg tcaggctcat ctccacccgt tccggcggat agtagccgcc
     2221 acgcccgggg cgatagccgc gataaccgcg cgggcgccag cggtcaggcc gccagcgtgg
     2281 cctcagcggc ggcgtgatca ggccgccgaa gttcggccgg atctgggcgt cgatgcccgc
     2341 ggccgcgtcc ggaaaggcct cggacgggct ggcggccgcc gccacgatca gggccgctcc
     2401 cgcggcggtc agaaagcggg tgagcgtctt catcgtgcgt agtccctgtc gcggggcgcc
     2461 tgccggggcg agcgggacac ctgggccagc acctgctgga ggcgtcccac cgacggcttg
     2521 aagcccgaaa gcgcggagtc gatctggtag acgaccgcct tggccttgtc ctggtcggcc
     2581 acccccgcga cgccgagcga gaagtaggtg gacatggcgt acttggcctc gcccgaaccc
     2641 tgacggtcgg cctcggcgaa ccagtggaag gagcgggcgt agtcgcgcgg cacgccgacc
     2701 ccctccgcat acatcaccgc cagcaccagc tgcgccttgg gcgacccgtt gacggcggcg
     2761 tactggatga tccggacctt ttcgatcggc gccagcaccc cggtttgcgg ccggccgatg
     2821 cgcgcctgga agtcctgcac ctcctgcgga tagagggcgt cctcgttggt cacggtgcgc
     2881 ggcgtcgagc gcagataggc cagcgcctcg ccgatgtgga cgaacggcaa ctcgcgcacc
     2941 cgggccaggg cgacctgctg ggcgtcgccg atctggtcgc tctcgtcgga gtgcggcacg
     3001 cccgaggacg gcgattccgg cggatagaac tcgtagggcg gaatccagcg tttggccttg
     3061 gcctcgacat actggccggc ggcggcgcgc tgggccgagg accgctcgaa gtccttgagc
     3121 atgacgtagg cgccgacgtc gccggtctgc acggcgaggt aattgtagag ataggcgtcg
     3181 acgtcgttgc gcggaaacag gttcagcacc tgcggcgtgc cgggcttgta cggcttgccg
     3241 cgggccgccg cggcctgtgg attgtcggac ggctcgccga acggcccgaa gaagttgtca
     3301 tgcatccgcg ccagggtccg gtagccgccc gccccttcgg tcgagaggat atagatcacg
     3361 cggctgcgga ccttctcctg ctcctcggag gacatctgcg acagaagccg gtcgagcgcg
     3421 cgataggcga tgccgcgttc gaccgcgcgg cagtcgtcgt agcgggcgat ggccttctgt
     3481 tcgccctgcg gcgagcgtcg gccataggcg gagatcggcg cgtagccgtc gccgttagac
     3541 agggccacgg cgtaccagac ggccgcctcg accggatcct cgaggttctt gtcggccgcc
     3601 cgctggcctt cgtagcgacg ggccagctcg agctgcgaga agaagtcgcc ggtgaacccg
     3661 cgacggcgca gggtgcgaat ctcgagttgc tgggtgttga actggggcgc cacgccggtc
     3721 ggaatctgcg gccttggaca ggccgtatcg cccggcttgc cgaaaaagct ctcggcgccg
     3781 gtgtcgcagc cgacgagcgg aatcaccacc gcggcgacga gcgccgcaac cgacgcgccg
     3841 cggatcagcg ggccttgcgc cgccccgaac ggacgaccgt cggcgtcgct ggcatcccgc
     3901 atactgtcac ccccacccag ctcgccgtta accattcggc aaccgcgact cttcgctggg
     3961 ctccccctgg agtcaagctc gccagcgagg cgtcgacgac atcaatcccc gtcccggccg
     4021 tcggagtgaa ccatgttcgc ccgccacgcg aagttattta ccctgtcgcg cgcggcgcgc
     4081 ctcgccgacg atcggttttc gcccagcttt ggccgcagag ccggagcgat catcgttcag
     4141 ggaccaggag tcaggggaat cagatgcgcc tgtcgaggac cgtccgggaa ctgaccaccg
     4201 tcgcggcgct cgcggccttg accgcggcct cggcgccgcc gttccagacg gccgcc
//
LOCUS       DAORNX010000256         4248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15511_length_4248_cov_7.007870, whole genome shotgun sequence.
ACCESSION   DAORNX010000256 DAORNX010000000
VERSION     DAORNX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15511_length_4248_cov_7.007870"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..807
                     /gene="pdxH"
                     /locus_tag="PLO65_11230"
     CDS             139..807
                     /gene="pdxH"
                     /locus_tag="PLO65_11230"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRD28862.1"
                     /translation="MTDKSDLIPPSPDEADYVRQVTEAEPLPLLPEEDPIALFETWLK
                     AAHQSEVNDGNAMALATVDADGLPDVRMVLLKDVDADGFVFYTNLESAKGRQLAAKPA
                     AALLFHWKSLRRQVRVRGAVTPVSEAEADAYFASRARHSQIGAWASDQSRPMTEPLQL
                     GRKVAEAGLRFGLGKVPRPPHWSGFRLAPRTIEFWRDRPFRLHERLVFERQGAGWTTR
                     RLFP"
     gene            complement(788..1423)
                     /locus_tag="PLO65_11235"
     CDS             complement(788..1423)
                     /locus_tag="PLO65_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28863.1"
                     /translation="MATSAFGETRSERKRKLILAAARDVFVREGFAHTGMEQVARGAG
                     VSTATLYAHFPGKADLFRVVVEESIADIAARVRRSAQTDGDARTRLSKFGQAYAEFYC
                     TPTSRATFRMVIAERRRFPDLADHFQERGRAELGGTLLEIIDGLAADGEIAVEKPCWA
                     AGQLQGMIEHATLILGLVAGDEVMPVRSIETIVAEAVETFLARYQVRERAA"
     gene            complement(1493..2176)
                     /gene="udk"
                     /locus_tag="PLO65_11240"
     CDS             complement(1493..2176)
                     /gene="udk"
                     /locus_tag="PLO65_11240"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRD28864.1"
                     /translation="MSDPSSAPPAAPLLIAVAGGSGSGKSTLAAALEARLPAGTVTLV
                     IEDAYYGDHGGKPGFDAARFDFDDVSAKDHALLARHLAALRAGETVQAPVYCFETHRR
                     LAPAATIRPAPLIVVEGAHLLCTPDLAALFDLRVFVDTPADVRFIRRLMRDQAQRGRT
                     ADSVIQQYLATVRPAHERLIEPSRTRADIVIADTRGAVIPDDPTEFDRLLAPVLAHPL
                     LNAYAAYSN"
     gene            2314..>4248
                     /locus_tag="PLO65_11245"
     CDS             2314..>4248
                     /locus_tag="PLO65_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRD28865.1"
                     /translation="MQGLMQQSALTVDTILDHARRWHGDREVVSRSVEGPIVRETYAQ
                     MHDRARRVSNALLGLGIRLGDRVATLAWNSGRHMETWYGIMGIGAVCHTLNPRLHPDQ
                     IAWIIRHAEDRIIFVDLTFVPILEQILADCPSVEHVIVFTDSHHMTPFQIHGVPTLNW
                     KGAHTYETLVEGSTPDVAWGGFDENTACGLCYTSGTTGDPKGVLYSHRSNYIHTLVGL
                     QRDVLGVSALDTVLPVVPMFHANAWGIAFAGPAVGAKLVMPGSKMDGESIHELLETEG
                     VTFSAAVPTVWQMLLQHLDKTGGKLTTLKRVVIGGSACPEAIIRGFHDRYGVDVLHAW
                     GMTETSPLGTLGTANARIAALPYDQQMTYKLKQGRPPLGIDLKLTDDEGRELPHDART
                     FGNLKVKGPFVVREYFKGAGGNILDRDGYFDTGDVATIDPEGFMQITDRAKDVIKSGG
                     EWISTIDIENIAMGHPKVALAAVIGMPHPKWDERPLLLIKLKDGETATKEEMLRFLEG
                     KIAKWWMPDDVAFVDDIPLGATGKIDKKLIRERMKDYVLPATPVLAAAVVAEKAAPDP
                     VLRAPEAPAGKSEAPTSVAAAVGAEEANSARVEETTVAPPQPAAPADQASHAWSASEP
                     EVDGPVPPPLSGMPDMTPIGA"
BASE COUNT          698 a         1494 c         1441 g          615 t
ORIGIN      
        1 cggtgcgacc ccgagccgtg cgcgcgccga tcgcgcggtc atgctgcgcc cgtccccggt
       61 catcgccggc agggtgaacg gacagggtca atggtcagtt aagctgaaac cacgcgcgcg
      121 ccgcgctata ggaggcggat gaccgacaag tccgacctga tcccgccatc gcccgacgaa
      181 gccgactacg tgcggcaggt gaccgaggcc gagcctctgc ccctgttgcc cgaggaggac
      241 ccgatcgccc tgttcgagac ctggctcaag gcggcccacc agtcggaggt caacgacggc
      301 aacgccatgg ccctggccac ggtcgacgcc gacggcctgc cggacgtgcg catggtcctg
      361 ctcaaggacg tcgacgccga cggcttcgtt ttctacacca acctcgaaag cgccaagggt
      421 cgccagctgg cggccaaacc ggccgcggcc ctgctgttcc actggaagtc cctgcgccgc
      481 caggtgcggg tgcgcggcgc ggtcacgccg gtcagcgagg cggaagccga cgcctacttc
      541 gcgtcccgcg cccgtcacag ccagatcggc gcctgggcct ccgaccagtc tcgtcccatg
      601 accgagccgc tgcagctggg ccggaaggtc gccgaggccg gcctgcgctt cggcctgggc
      661 aaggtgcctc ggccgcccca ctggtcgggc ttccgtctcg cgccgcggac catcgagttc
      721 tggcgcgacc ggccgttccg cctgcatgag cggctggtgt tcgagcgcca gggcgcgggc
      781 tggacgacta ggcggctctt tccctgacct ggtagcgggc gaggaaggtc tcgacggcct
      841 cggcgacgat cgtctcgatc gacctgacgg gcatcacctc gtcgccggcg accaggccga
      901 ggatcagggt ggcgtgttcg atcatgccct gcagctggcc ggcggcccag cacggctttt
      961 cgacggcgat ttcaccgtcg gcggcgaggc cgtcgatgat ctccagcagc gtgcccccca
     1021 gttcggcccg gccccgctcc tggaaatggt cggccaggtc agggaaccgc cgtcgctcgg
     1081 cgatgaccat ccggaacgtc gcccgcgagg tcggggtgca gtagaattcg gcgtaggcct
     1141 gaccgaattt cgacagccgc gtccgggcgt cgccgtcggt ctgggctgac cgacgaacgc
     1201 gggcggcgat gtcggcgatg gactcctcga ccaccacccg gaacaggtcc gccttgccgg
     1261 gaaagtgcgc atagagggtg gccgtcgaga caccggcgcc cctggcgacc tgctccatgc
     1321 ctgtatgggc gaaaccctcg cggacaaaga catcccgggc ggccgcgaga atgagtttgc
     1381 gcttccgttc cgaccgggtc tccccaaacg ctgacgtcgc catgccacct cgaaatacgc
     1441 tgcccggact cagcctatag gcgaaaaaca gggagccgca aggatttcag cctcaatttg
     1501 agtaggcggc gtaggcgttg agcaggggat gggccagcac cggcgccaga agacgatcga
     1561 actcggtcgg atcatcgggg atcaccgcgc cgcgggtgtc ggcgatgacg atgtcggcgc
     1621 gggtcctgga cggctcgatc agccgttcgt gagccggccg cacggtggcg agatactgct
     1681 ggatgaccga gtcggccgtc cttccccgct gggcctggtc gcgcatcagc cggcggatga
     1741 accgcacgtc cgccggcgtg tcgacgaaca cgcgcaggtc gaacagcgcc gccaggtccg
     1801 gcgtgcacag cagatgggcg ccctcgacga cgatcaacgg cgccggcctg atcgtcgcgg
     1861 cgggcgccag gcggcggtgc gtctcgaagc agtagaccgg cgcctggacg gtctcgccgg
     1921 cgcgcagggc ggccaggtgc cgggccagca gggcatggtc cttggcggag acgtcgtcga
     1981 agtcgaaccg cgccgcgtcg aaacccggct tgccgccgtg gtcgccatag taggcgtcct
     2041 cgatgaccag ggtcacggtt ccggccggca gccgcgcctc cagcgccgcg gccagggtgc
     2101 tcttgcccga ccccgagccg ccggcgacgg cgatcagcag gggggcggcg ggcggggctg
     2161 acgacgggtc gctcatgccg cctatcggcc acaaaccgac gggtttgcca attgtcgcca
     2221 tggtgaattt tctacccctg cgagccccgc gttgcccgcg cggcctcgcc ccgctagggt
     2281 tccgcccaca aggaaaaacg ggggagagcg gccatgcagg ggctgatgca gcagtcggcg
     2341 ttgaccgtcg acacaatcct ggatcacgcc aggcgctggc acggcgatcg cgaggtcgtc
     2401 agccggtcgg tcgaggggcc gatcgtgcgc gagacctacg cccagatgca cgaccgggcc
     2461 cggcgcgtct ccaacgccct gctcggtctg ggcatccgcc ttggcgaccg ggtcgccacc
     2521 ctggcctgga actcgggccg gcacatggag acctggtacg gcatcatggg catcggggcg
     2581 gtctgccaca cgctcaatcc gcgcctgcac ccggaccaga tcgcctggat catccgccac
     2641 gccgaggacc ggatcatctt cgtcgacctg accttcgtgc cgatcctcga acagatcctc
     2701 gccgactgtc cgtcggtcga gcatgtgatc gtcttcaccg acagccatca catgacgccg
     2761 ttccagatcc atggcgtgcc gacgctgaac tggaagggcg cccacaccta cgagaccctg
     2821 gtcgagggca gcaccccgga cgtcgcctgg ggcggtttcg acgagaacac cgcctgcggc
     2881 ctctgctaca cttccggaac gacgggcgac cccaaggggg tgctctattc ccaccgctcc
     2941 aactatatcc acaccctcgt cggcctgcag cgcgacgtgc tcggcgtgtc ggcgctcgat
     3001 acggtcctgc cggtggtgcc gatgttccac gccaacgcct ggggcatcgc cttcgccggc
     3061 ccggccgtcg gcgccaagct ggtgatgccc ggttcgaaga tggacggcga gtcgatccac
     3121 gaactgctgg aaaccgaggg cgtcaccttc tccgccgccg tgccgaccgt ctggcagatg
     3181 ctgctgcagc atctcgacaa gaccggcggc aagctgacca cgctcaagcg ggtggtgatc
     3241 ggcggttcgg cctgcccgga ggcgatcatc cgcggcttcc acgaccgcta tggcgtcgac
     3301 gtgctgcacg cctggggcat gaccgagacc tcgccgctcg gcaccctcgg caccgccaac
     3361 gcccgtatcg ccgccctgcc gtacgaccag cagatgacct acaagctgaa acagggccgg
     3421 ccgccgctcg gcatcgacct gaagctgacc gatgatgagg gccgagagct gccgcacgac
     3481 gcccgcacgt tcggcaacct gaaggtcaag ggcccgttcg tggtccgcga gtacttcaag
     3541 ggcgccggcg gcaacatcct cgaccgcgac ggctatttcg acaccggcga cgtcgccacg
     3601 atcgatcccg aaggcttcat gcagatcacc gaccgcgcca aggacgtgat caagtccggc
     3661 ggcgaatgga tcagcaccat cgacatcgag aacatcgcca tgggccaccc caaggtggcg
     3721 ctggcggcgg tcatcggcat gccgcatccg aagtgggacg agcggccgct gctgctgatc
     3781 aagctcaagg acggcgagac ggcgaccaag gaagagatgc tgcgctttct cgagggcaag
     3841 atcgccaaat ggtggatgcc cgacgacgtg gccttcgtgg acgacatccc gctgggcgcc
     3901 accggcaaga tcgacaagaa gctgatccgc gaacggatga aggactacgt cctgcccgcc
     3961 acgcctgtcc tggccgccgc cgtggtggcg gagaaggcgg cgcctgaccc ggtgctgcgg
     4021 gccccggagg cccccgcggg aaaatctgag gccccgacca gcgtggctgc tgctgttggg
     4081 gccgaggaag cgaactccgc cagggtcgaa gagaccaccg tcgcgccgcc gcaaccggcc
     4141 gcgcccgccg accaggcctc gcacgcctgg tcggcctccg aacccgaggt cgacggcccc
     4201 gtgccgccgc cgctgtcggg catgcccgac atgacgccga tcggggcg
//
LOCUS       DAORNX010000257         4244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15530_length_4244_cov_8.754118, whole genome shotgun sequence.
ACCESSION   DAORNX010000257 DAORNX010000000
VERSION     DAORNX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15530_length_4244_cov_8.754118"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..3150)
                     /locus_tag="PLO65_11250"
     CDS             complement(298..3150)
                     /locus_tag="PLO65_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD28866.1"
                     /translation="MSESLRLRSMLIASVSAAAMLGVAAPAFAQDTTVGEVVVTAQKK
                     EEAIQDVPIAVSAFSAESLEKAKIDGGPNLVLAVPNVNFSKGNFTGYNFQIRGVGSKL
                     VAGSGDAGTGIHLNNAPLTANNLFESEFFDVERVEVLRGPQGTLYGRNATGGVVNLIS
                     AKPTDDFAAMFRAEAGNFGTRKVRGMVNVPLGDMMALRVAGTMTQRDGYGRNLTTGND
                     IDDRDLWNTRITLAVNPSDTFRTYLMWDHFEEDDARSRIGKQLCVKDLGPGSVGGVGY
                     SGAFFGLIGQIQRGFFSQGCSAAPVNDPNILGTPNSQATLGGLFGTLYGFTTGDAFAG
                     QMQDPDIRNIQSAFDPIYRAKTDIVQFNISWDLTDELQLTALTGYSKNELYTRQDYNR
                     IVPNTQFNATPNPVNAYAAFGPVYTGVYAALFNPTAQPYGTVQDPQVGAQSRFTTFDI
                     SGGESTNWSQELRLQSNFDGPFNFNAGVIYIDFKSDGDYYVMGNTLTAATQVENALPA
                     VASLLGCSSATPCYIDPNADPTRTGRNYYDNTGAYHLKSRAAFGELYWQLFDNFKITG
                     ALRYTHDMKSVENRPVALFVPGGPGIPLSTTTPYLTADFEEVTGRFGFDWKPEVGFTD
                     ETLVYLFYSRGYKAGGVNPACSAVVGCPPPTFDPEFVDAIELGTKNTLLGGAMVLNLT
                     GFKYDYIGYQVSKIVNRTSVNENIDAEIKGLELETVWSPMDGLRLNMNIGYLQTEITG
                     GTSIDTFNRTQGNPLLTLVKSSAASNCVVTTANAALALTVSNATNNPFALLGVCTPVT
                     TATAGTPLLPGGPLAVGTNAFGGLISDGVEVDLTGNELPNAPHYTLSLGAQYNWEFGG
                     GWEGTIRGDYYKQTETFSRIYNSAPDRLDSWENFNATFTVSNYESGWSLEVYGKNLMD
                     DSPLTDTYLTDDSSGLFRNGFYLEPRTYGVAVSKTF"
     gene            3525..>4244
                     /gene="queC"
                     /locus_tag="PLO65_11255"
     CDS             3525..>4244
                     /gene="queC"
                     /locus_tag="PLO65_11255"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRD28867.1"
                     /translation="MPPIRPSADGALVLFSGGQDSAVCLAWALDRYGRVETVGFDYGQ
                     RHIVEMEARQRVRAGLRAARPDWAARLGEDHVLDLRGFGAVAESALTSDRAIEMTARG
                     LPSTFVPGRNLVFLVYAAALADRRGLGALVGGMCETDYSGYPDCRRDTIDAMQQALNL
                     GMEQAFRVETPLMRLTKAQTWQLAAALGGESLVSLIVEESHTCYRGERGDLHPWGHGC
                     GACPACELRAAGWDAWVAAGRP"
BASE COUNT          629 a         1325 c         1520 g          770 t
ORIGIN      
        1 caggatgacg tcgcgcgcga ccaggcccgc cggctggtcg ccatcgacga cagccaggac
       61 ggcggcgtcc ggctcggccg cgaaacgcgc ggcggcctgg gcgcaggtcg acccggccgg
      121 gatcggcgcg cggcggtcag cgaaggtggc gaaggtgttc atcagggccg cacgcgaaga
      181 tcaggacgat ccccaccatg accgaatgcg cttgcagaga ggttaagccc tcaatggaca
      241 aagaaaaacg gcggggacca gggtccccgc cgttccgctc ttcactggcc tttcggctta
      301 gaaggtcttg ctcaccgcca caccgtaggt gcggggctcg aggtagaagc cgttgcggaa
      361 cagacccgac gagtcgtcgg tcaggtaggt gtccgtcagc ggggagtcgt ccatcaggtt
      421 cttgccgtag acttccagcg accagccgct ttcgtagttg gacacggtga aggtcgcgtt
      481 gaagttctcc cagctgtcca ggcggtcagg cgcggagttg tagatgcgcg agaacgtctc
      541 ggtctgcttg tagtagtcgc cgcggatcgt gccttcccag ccgccgccga actcccagtt
      601 gtactgggcg ccgagggaca gggtgtagtg cggcgcgttg ggcagctcgt tgcccgtcag
      661 gtcgacttcc acaccgtcgc tgatcaggcc gccgaaggcg ttggtgccga ccgcgagcgg
      721 accgcccggc agcagcggcg taccggcggt ggccgtggtc accggggtgc agacgccgag
      781 cagggcgaac gggttgttgg tcgcgttgga gaccgtcagg gccagcgcgg cgttcgccgt
      841 ggtgaccacg cagttggacg ccgcgctcga cttcaccagc gtcaggagcg gattgccctg
      901 ggtccggttg aaggtgtcga tcgaggtgcc gccggtgatc tcggtctgca ggtagccgat
      961 gttcatgttc agccgcaggc cgtccatcgg cgaccagacg gtttccagtt cgagaccctt
     1021 gatctcggcg tcgatgttct cgttcaccga ggtccggttc acgatcttcg acacctggta
     1081 gccgatgtag tcgtacttga agcccgtgag gttcagcacc atggcgccgc ccagcagggt
     1141 gttcttggtg cccagctcga tcgcgtcgac gaactccgga tcgaaggtgg gcggcgggca
     1201 gccgaccacg gccgagcagg ccgggttgac gccgccggcc ttatagccgc gcgagtagaa
     1261 caggtagacc agggtctcgt ccgtgaagcc cacttcaggc ttccagtcga agccgaagcg
     1321 accggtcact tcttcgaagt cggcggtcag gtacggcgtc gtggtgctga gcgggatgcc
     1381 cgggccgccc ggaacgaaca gggccaccgg acggttctcg accgacttca tgtcatgggt
     1441 gtagcgcagg gcgcccgtga tcttgaagtt gtcgaacagc tgccagtaga gctcgccgaa
     1501 ggccgcgcgg gacttcaggt gataggcgcc ggtgttgtcg tagtagttgc ggccggtgcg
     1561 ggtcggatcg gcgttcgggt cgatgtagca gggcgtggcg ctcgagcagc cgaggaggct
     1621 ggccacggcc ggcagggcgt tttcgacctg ggtggcggcg gtgagggtgt tgcccatcac
     1681 atagtagtcg ccgtcagact tgaagtcgat gtagatcacg ccggcgttga agttgaacgg
     1741 cccgtcgaag ttcgactgca ggcgcagttc ctgcgaccag ttggtgcttt cgccgcccga
     1801 gatgtcgaac gtcgtgaagc ggctctgggc gccgacctgc gggtcctgga ccgtgccgta
     1861 gggctgggcc gtcgggttga acagggccgc gtagacgccc gtgtagaccg ggccgaaggc
     1921 ggcataggcg ttgaccgggt tcggcgtcgc gttgaactgg gtgttcggca cgatccggtt
     1981 gtagtcctgg cgggtgtaca gctcgttctt gctgtagccg gtcagggcgg tcagctgcag
     2041 ttcgtcggtc aggtcccacg agatgttgaa ctgaacgatg tcggtcttgg cccggtagat
     2101 cgggtcgaag gccgactgga tgttgcggat atccgggtcc tgcatctggc cggcgaaggc
     2161 gtcgccggtg gtgaagccgt acagggtgcc gaacaggccg cccagggtgg cctggctgtt
     2221 gggggtgccc aggatgttcg ggtcattgac cggggcggcc gaacagccct ggctgaagaa
     2281 gccgcgctgg atctggccga tgaggccgaa gaacgctccg gaatagccga cgccgccgac
     2341 gctgccgggg ccaaggtcct tcacgcacag ctgcttgccg atacgcgagc gggcatcgtc
     2401 ttcctcgaag tggtcccaca tcaggtaggt gcggaacgtg tcgctcgggt tgaccgccag
     2461 cgtgatgcgg gtgttccaca ggtcgcggtc gtcgatgtcg ttgccggtcg tcaggttgcg
     2521 gccgtagccg tcccgctggg tcatcgtgcc agcgacgcgc agggccatca tgtcgcccag
     2581 cggcacgttg accatgccgc ggaccttgcg ggtgccgaag ttgccggcct cggcgcggaa
     2641 catcgccgcg aagtcgtcgg tcggcttggc ggagatcagg ttgaccacgc cgccggtggc
     2701 gttgcggccg tacagggtgc cttgcggacc gcgcagcact tccacgcgct cgacgtcgaa
     2761 gaattccgac tcgaacaggt tgttggcggt gagcggggcg ttgttcaggt ggatgccggt
     2821 gccggcgtcg cccgatccgg cgaccagctt ggagccgacg ccgcggatct ggaagttgta
     2881 gccggtgaag ttccccttgg agaagttcac gttcggcacg gccagaacca ggttcggacc
     2941 accgtcgatc ttggccttct cgaggctttc agcgctgaag gccgagacgg cgatcggcac
     3001 gtcctggatc gcctcttcct tcttctgagc ggtcacgacg acctcgccga ccgtcgtgtc
     3061 ctgggcgaaa gcgggtgcgg cgacgccaag catggcggcg gccgacacgc tggcgatgag
     3121 catactccgc aggcgcagag attcggacat tcgtaaccct ccccggacgg cgcgacccct
     3181 tatggggggt tcggcggcgt cgttgtttcc tcatgtatcg cgcgattagt taccgccggg
     3241 gtcgcgcgac acattccccc gcagcttctt cgaattgcat acgggcgttt gatgtgtgtc
     3301 aaccatcgcc gtcacgttac cgccgtcaag ttgccgcttt tcaccttggt gttgccgccc
     3361 tgcttcgcct tgtgacgctt gggaaacgac ttcttctcgt catgagggag ccgctgcgcg
     3421 tcaaagaggc gcacaacggc ccggcggacg tttcggcggc ggctccggtc gtgtacagct
     3481 cgcgccgttc caccccgccc ccgatcccgc cgcgaagccg ccagatgcct ccgattcgtc
     3541 cgtccgccga tggtgcgctc gttctgttct cgggcgggca ggattcggcc gtctgcctgg
     3601 cctgggcgct ggaccgctac gggcgcgtcg agacggtcgg attcgactac ggccagcgcc
     3661 acatcgtcga gatggaagcc cgtcagcggg tgcgggcggg tctgcgggcg gcgcgtccgg
     3721 actgggccgc gcgactcggc gaggatcacg tgctcgatct gcgcggcttc ggggcggtgg
     3781 ccgagtccgc cctgaccagc gatcgcgcca tcgagatgac cgcccgcggc ctgccctcga
     3841 ccttcgtgcc cgggcgcaat ctggtgttcc tcgtctatgc cgcggccctg gccgaccggc
     3901 gcggtctcgg cgcgctcgtc ggcggcatgt gcgagaccga ctactccggc tatcccgatt
     3961 gccgccgcga cacgatcgac gcgatgcagc aggccctcaa cctcggcatg gagcaggcct
     4021 tccgcgtcga gacgccgctg atgcggctga ccaaggcgca gacctggcag ctggccgcgg
     4081 ccctgggcgg cgagtcgctg gtgtcgctga tcgtcgagga aagccacacc tgctaccgcg
     4141 gcgagcgggg cgacctgcat ccctggggcc acggctgcgg cgcctgtccc gcctgcgagc
     4201 tgcgcgcggc cggctgggac gcctgggtcg ccgcgggccg tccg
//
LOCUS       DAORNX010000258         4239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15552_length_4239_cov_6.395076, whole genome shotgun sequence.
ACCESSION   DAORNX010000258 DAORNX010000000
VERSION     DAORNX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15552_length_4239_cov_6.395076"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>593)
                     /locus_tag="PLO65_11260"
     CDS             complement(80..>593)
                     /locus_tag="PLO65_11260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD28868.1"
                     /translation="IIAAAARLRAEGREDFIVVFAGDDQGRTDYREEILAAIAAAGLE
                     DCVKVVGHCDDMPAAFLLADVALLPTLKPESFGRTAVEPQVMERPVIVSDHGGARETV
                     VDGETGWRAPPGDVAAWARAMKTAIEAGGERRRAMGELGAARARRLYSVDAMCDATLE
                     VYARVLEARA"
     assembly_gap    594..620
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<621..1258)
                     /locus_tag="PLO65_11265"
     CDS             complement(<621..1258)
                     /locus_tag="PLO65_11265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD28869.1"
                     /translation="MTTLPPNFTLLQVTPELDTGGAEQTTIDVARAVIAAGGKALVAT
                     RGGRMTSRLLADGGRLVEMPVQSKNPLVMLGNAARLIDLIRSEKVSLVHARSRAPAFS
                     ALWAAHAAGVPFIATYHGVYKANNGLKRWYNAVMTRGDLVIANSDYTRDHVLAEHHLD
                     PARIVTIPRGVDLARFDPRTVSRDRVEALRTAWGLPADRSRLVFLLGGRLTR"
     gene            1457..2206
                     /locus_tag="PLO65_11270"
     CDS             1457..2206
                     /locus_tag="PLO65_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28870.1"
                     /translation="MKEETGFLDRGDGQQLAWRRVAGRGPTVVWLGGFMSDMTGTKAQ
                     ALADRALAQGRAFLRFDYLGHGQSSGRFRDGTITRWRADALAAIAALTEGPLVLVGSS
                     MGGWISCLVAAEIPERLHAMVLIAPAADFTEALMRPGLPPEALAAIERDGEWIRPSLY
                     EPTGYPITRGLLEDGARWSILGAPVPVDAPVRILQGREDPDVPWLHALALANAIRSED
                     VVFTLIKDGDHRLSREQDIARLIATVAEAGG"
     gene            2281..2541
                     /locus_tag="PLO65_11275"
     CDS             2281..2541
                     /locus_tag="PLO65_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28871.1"
                     /translation="MSDPQPLSPEQEAVRDKIVAAGEGWGSTEQQVDIAVRAELGDTP
                     VYDLRFPRGGGGYVGDTEYIPPDGGPPITIPGDGASAYDCFW"
     gene            2548..3045
                     /locus_tag="PLO65_11280"
     CDS             2548..3045
                     /locus_tag="PLO65_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28872.1"
                     /translation="MSMRVGLLALALASIAAACGPGAEAACNVDGARYALRGSPAFTA
                     SFRQVPGSRSAHDLIFDVRSAKTGRTFSFRINRGNGYGEATLSPTRGGMSGQVELYTL
                     NDAAEFTDYFGEVEGPAPRQLLAPKLGPVLWYQADALTGASNSARERMPRAFFDRVSC
                     GPAAR"
     gene            complement(3050..3913)
                     /locus_tag="PLO65_11285"
     CDS             complement(3050..3913)
                     /locus_tag="PLO65_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD28873.1"
                     /translation="MTTRALVLGGGGPVGIAWETGLIAGLAEAGAHVHHADWILGTSA
                     GSVCGALLATGHTPEAMLRAEFANAERAKEAQAKRQGKAPDLTPLMSVMARRPPQGEM
                     PPELMLELGQLSLNGQTVSEEVFIAGFGPIAEEQPWPQRYACTAVDTATGEFVVWSSE
                     TAAPLGRGVASSCSVPAIFPAITIDGRRYMDGGMRSGTNADLAKDHGKVIVVAVMPPA
                     FAPMMLPRLEAEMAVIRNHGGEALLLVPDAACGEAFGGNLMDSGNREAIARAGFAQGQ
                     AEAARVRAFWG"
     gene            complement(3910..>4239)
                     /locus_tag="PLO65_11290"
     CDS             complement(3910..>4239)
                     /locus_tag="PLO65_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28874.1"
                     /translation="RIHVDDLAAGLAASIARPRAGASYNLCDDEPAPSHQVNAFAARL
                     LGLPPPAPVAFEDAGLSPAARRFWSENKRVSNSLAKAELGWRPLYPTYREGLTAIHAA
                     ESGGSDR"
BASE COUNT          613 a         1481 c         1479 g          639 t
ORIGIN      
        1 cgcttcatgg cggccagcga cagcacgaac tcggcgatcg agcccgcctg gatcaccaga
       61 accctcttga tctcgcgcgt cacgctctcg cctccagaac gcgtgcatag acctcaagcg
      121 tggcgtcgca catggcgtcg accgaataaa gtcgtctcgc gcgggccgcg cccagttcgc
      181 ccatcgcgcg acgccgctcg ccgccggcct cgatggccgt cttcatggcg cgggcccagg
      241 ccgcgacatc accgggcggg gcgcgccagc cggtctcgcc gtcgacgacc gtttcgcggg
      301 cgccgccgtg gtcggacacg atcaccggcc gctccatgac ctgcggctcg accgccgtgc
      361 ggccgaacga ctcgggcttg agcgtcggca gcaaggccac gtcggccaga aggaaggcgg
      421 ccggcatgtc gtcgcagtga ccgaccacct tcacgcagtc ctccagcccc gccgcggcga
      481 tggcggcgag aatctcctcg cggtagtcgg tgcggccctg gtcgtcgccg gcgaagacga
      541 cgatgaaatc ctcgcggccc tcggctcgca gccgcgccgc cgccgcgatg atcnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn atgcgggtca gccgcccgcc gagcaggaac accagcctcg
      661 accggtccgc cggcagcccc caggccgtcc gcagcgcctc gacccggtcg cgggacacgg
      721 tccgcgggtc gaagcgggcc aggtccacgc cgcgcgggat ggtgacgatg cgcgccgggt
      781 ccaggtggtg ctcggccagc acatggtcgc gcgtgtagtc ggagttggcg atcaccaggt
      841 cgccgcgggt catgaccgcg ttgtaccagc gcttgaggcc gttattggcc ttgtagaccc
      901 cgtggtaggt ggcgatgaac ggcacgccgg cggcgtgggc cgcccacagg gcgctgaagg
      961 ccggcgcgcg cgagcgggca tgcaccaggc tgaccttctc gctgcggatc aggtcgatca
     1021 gccgcgcggc gttgcccagc atgaccagcg ggttcttcga ctgcaccggc atctcgacca
     1081 gccggccgcc atcggccagc aggcggctgg tcatccggcc gccgcgcgtg gcgaccagcg
     1141 ccttgccgcc cgcggcgatc accgcgcgag cgacatcgat ggtcgtctgt tcggcgcccc
     1201 cggtgtcgag ctcgggggtt acctgcagca gcgtaaaatt gggcggcaag gtcgtcaccg
     1261 cccttgcata ccctgtaacg ccgtcaggta aacacacccc gccctcacac agccgtcatg
     1321 gcccggcttg ttcgggccac ccgctccctt cgtcatggtc ggacttgttc cgaccaccca
     1381 tgcgtgatga gcctgggggt gaagcgccgt gttcacgggc gtcggaacac gtcggacgat
     1441 gacggaagct ggattgatga aagaagaaac gggttttctc gatcggggcg acggccagca
     1501 gctggcctgg cggcgcgtcg ccgggcgcgg gccgaccgtg gtgtggctgg gcggtttcat
     1561 gtcggacatg accgggacca aggcccaggc cctggcggac cgggccttgg cgcagggccg
     1621 cgccttcctg cgcttcgact atctcggcca cggccagtcg agcgggcgct ttcgcgacgg
     1681 cacgatcacc cgctggcggg ccgacgccct ggcggcgatc gcggccttga ccgaaggacc
     1741 gctggtgctg gtcggttctt cgatgggcgg ctggatcagc tgcctggtcg ccgcggagat
     1801 tcccgagcgc cttcacgcca tggtgctgat cgccccggcc gccgacttca ccgaggcgct
     1861 gatgaggccg ggcctcccgc cggaggccct cgcggcgatc gaacgggacg gggagtggat
     1921 caggccgtcg ctgtatgagc cgaccggcta tccgatcacc cgcggcctgc ttgaggacgg
     1981 ggcccgctgg tcgatcctcg gcgcgccggt cccggtcgac gcgccggtgc gcatcctgca
     2041 gggccgcgag gacccggatg tgccgtggct gcacgcgctg gcgctggcca acgcgatccg
     2101 gagcgaggac gtggtcttca ccctgatcaa ggacggcgac catcggctca gccgggagca
     2161 ggacatcgcg cggctgatcg caacggtggc ggaggctggc ggctaggccg aaattgttgt
     2221 tggcagcagg cggcgataca ccgtcaggat gtattttcat ggaagcagcc ggcgccatcc
     2281 atgagcgatc cgcagccact aagtccggag caggaggccg tgcgcgacaa gatcgtcgca
     2341 gcgggtgagg ggtggggctc caccgaacag caggtcgaca tcgcagtgcg ggccgaactc
     2401 ggcgacacgc ccgtctacga tttgcgcttc ccccgagggg gaggcggcta tgtcggcgac
     2461 accgagtata ttccgcccga tggaggcccc ccgatcacaa tccccgggga cggcgcgtcc
     2521 gcctacgact gtttctggtg attgggcatg agcatgcggg tcggactact cgccttggcg
     2581 ctggcttcaa tcgccgcggc ctgtgggccc ggggccgaag ccgcctgcaa tgtcgatggc
     2641 gcccgctatg cgctacgcgg cagcccggcg ttcacggcgt cgttccgtca ggtccccggc
     2701 tcccggtcag cgcacgatct gatcttcgat gtccgctctg caaagaccgg ccgaacattc
     2761 agcttccgga tcaatcgcgg aaacggctac ggcgaggcga cgctctcgcc gacgcgcggc
     2821 gggatgtccg gccaggtcga actctacacg ctgaacgacg cggccgaatt caccgattac
     2881 ttcggggagg tcgaggggcc ggcgccgagg cagttgctgg cgccgaaact ggggccggtc
     2941 ctctggtatc aagccgatgc gttgaccggc gcatccaaca gcgcgcgaga acggatgccc
     3001 cgcgccttct tcgatcgcgt gtcctgcggt ccggcggcgc gttagactcc tagccccaga
     3061 acgctcgcac ccgcgcggct tccgcctggc cctgggcgaa gcccgcccgc gcgatggctt
     3121 cccggttgcc gctgtccatc agattgccgc cgaacgcctc gccgcaggcc gcgtcgggga
     3181 ccagcagcag cgcctcgccg ccgtgattgc ggatcacggc catctcggcc tcgagccggg
     3241 gcagcatcat cggcgcgaag gccggcggca tgaccgccac gacgatcacc ttgccgtggt
     3301 ccttcgcgag atcggcgttg gtccccgagc gcatgccccc gtccatgtag cggcggccgt
     3361 cgatggtgat ggccgggaag atcgccggca ccgagcagga cgaggcgacg ccccggccga
     3421 gcggcgcggc ggtttcgctc gaccagacca cgaattcgcc ggtcgccgtg tccacagccg
     3481 tgcaggcata gcgctgcggc cagggctgtt cctcggcgat cggcccaaag ccggcgatga
     3541 acacctcttc gctgacggtc tgaccgttca gcgacagctg gcccagttcc agcatcagct
     3601 ctggcggcat ctcgccctgc ggcggccggc gagccatgac gctcatcagc ggcgtcaggt
     3661 cgggcgcctt gccctgcctc ttcgcctggg cttccttcgc ccgctccgcg ttggcgaact
     3721 cggcgcggag catggcctcc ggcgtatggc cggttgccag caacgcgccg cagaccgagc
     3781 cggccgaggt gccgagaatc cagtcggcgt gatggacatg ggcgccggcc tcggccaggc
     3841 ccgcgatcag cccggtctcc caggcgatgc ccaccggccc gccgccgccc agcaccagag
     3901 cccgtgtcgt catcggtcgc ttcctccgga ctcggcggcg tggatcgccg tcaggccttc
     3961 acgataggtc ggatagagcg gacgccaacc cagctcggcc ttggccaggc tgttggagac
     4021 gcgcttgttc tcgctccaga atcgtcgggc tgccgggctc aggccggcgt cctcgaacgc
     4081 caccggcgcc ggtggcggca ggcccagcag gcgggcggcg aaggcgttga cctgatgcga
     4141 gggggccggc tcgtcgtcgc agagattgta gctggcgccg gcgcgcggcc gggcgatcga
     4201 ggcggccagg cccgcggcga gatcgtcgac atggatgcg
//
LOCUS       DAORNX010000259         4237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15561_length_4237_cov_8.780488, whole genome shotgun sequence.
ACCESSION   DAORNX010000259 DAORNX010000000
VERSION     DAORNX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15561_length_4237_cov_8.780488"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PLO65_11295"
     CDS             complement(<1..1086)
                     /locus_tag="PLO65_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRD28875.1"
                     /translation="MLEPVTIEALNALGACLADAAGPNPPPAFDTWIDPLPDDADPAL
                     AAHIRHQALLRCYQARYVVLPETAPDGDLLERLDRHHGEDRMAALNAARPRLEAELIG
                     PLWDEANAAAGDGDALAYVDRLLPELRAVASDPFLDALAADEHRRDHYRNFLLQSSAD
                     LLAEASASALGVIGEFGAPQSALFRILIDEFGYGAHDHKHSVLFRATLRSFGLDDAYN
                     GYSRLFDSNTLMLHNAIHWLFQNPRNVFRQVGFLLFAETAYQRSTLFHARHLGRFHPE
                     AEARYFAEHAHIDLHHTRMVVEEVVRPFLDRFGPGPGREIIAGAELTRAVFARSGAGQ
                     LALIRAFAATADAAWGLGPFAGPPVTPA"
     gene            complement(1079..1729)
                     /locus_tag="PLO65_11300"
     CDS             complement(1079..1729)
                     /locus_tag="PLO65_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRD28876.1"
                     /translation="MVEITTLSELTIDGKLSLGKSASSKDLFLYSSDELKAWFHGQRA
                     ANDAIMVGANTVRIDDPELTVRHAPGRNPLRVIPSSDGRLPPGCTLLNDGVPTLVAVS
                     RRATPEAVAALRSHDHVEVVVCGEDQVDLEALMDQLDGRGVKTMIAEGGSELLSSLFK
                     AELVSRIIIKHIPVIAGAPDAPTYLNGATISLSRWRLEDLFIKGGVGVSVYRPADA"
     gene            1882..2805
                     /locus_tag="PLO65_11305"
     CDS             1882..2805
                     /locus_tag="PLO65_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD28877.1"
                     /translation="MQPIISIAGLSKTYAGGFQALKGVDLDIRPGEIFALLGPNGAGK
                     TTLIGVVCGIVNATAGTVIADGHDIVRDYRAARTKIGLVPQELSTDAFETVIATVKFS
                     RGLFGKPPNPAYIEKILRDLSLWDKKDNRIMTLSGGMKRRVMIAKALSHEPKILFLDE
                     PSAGVDVELRRDMWQMVRALRDSGVTIILTTHYIEEAEEMADRIGVINKGELILVEDK
                     DVLRRKLGKKQLTIQLREPLTALPAGLANDALSLSADGLDLTWSFDAQAEETGIAALL
                     KRLGEHGIDFKDLRTEESSLEEIFVNLVRAG"
     gene            2805..3566
                     /locus_tag="PLO65_11310"
     CDS             2805..3566
                     /locus_tag="PLO65_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD28878.1"
                     /translation="MNLHGVKAIYRFELARWFRTLMQSILAPVISTSLYFVVFGSAIG
                     SRMTTIDGVSYGAFIVPGLIMLSILTESISNASFGIYMPKWSGTIYELLSSPVSWIET
                     VMGYVGAAATKSVLLGLLILVTARLFVPFEIVHPVWMLGFLVLTAVTFSLFGFIIGLW
                     ADDFQKLQIVPTLIVTPLTFLGGSFYSISMLPPVWQTVTLFNPVVYLISGFRWAFYGK
                     ADVNVGVSVAATVGFMIACLIAIRWFFRTGYKMKA"
     gene            3573..>4237
                     /locus_tag="PLO65_11315"
     CDS             3573..>4237
                     /locus_tag="PLO65_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRD28879.1"
                     /translation="MSSDLFAPLTFAHGPAMKNRLMLAPLTNTQSHADGRMSDEEFRW
                     LTMRAEGGFGLTMTCASHVQRNGQGFPGQMGCWSDDHIEGLTRLAAAIKANDSLAVVQ
                     IHHAGMRSPAELIGEAPVCPSDNDEFKARALTAGEVEQLRDDFIAAAVRAKTAGFDGV
                     ELHGAHGYIIGQFLSPEINLRTDQWGGSPENRARLAIEILQGIRAQCGEGFQIGLRLS
                     PER"
BASE COUNT          707 a         1418 c         1425 g          687 t
ORIGIN      
        1 ggccggcgtc acgggcggac cggcgaatgg gccgaggccc caggcggcgt ccgctgtcgc
       61 ggcgaaggcc ctgatcagcg ccagctggcc ggcgccggac cgggcgaaca cggcccgggt
      121 cagttccgcg ccggcgatga tctcgcggcc gggaccgggg ccgaagcggt cgaggaacgg
      181 cctcaccacc tcctcgacga ccatccgggt gtgatgcagg tcgatgtgcg catgctcggc
      241 gaaatagcgg gcctcggcct cggggtgaaa gcgccccaga tggcgggcgt ggaacagggt
      301 cgagcgctgg taggcggttt cggcgaacaa caggaagccc acctgtcgga agacgttgcg
      361 cgggttctgg aacagccagt ggatggcgtt gtgcagcatg agcgtgttgc tgtcgaacag
      421 acgcgaatag ccgttgtagg cgtcgtcgag accgaacgat cgcagggtcg cgcggaacag
      481 cacgctgtgc ttgtggtcgt gcgcgccata gccgaactcg tcgatcagga tgcggaacag
      541 ggccgactgc ggcgcgccga attcgccgat cacccccagc gccgaggccg aggcctcggc
      601 cagcaggtcg gccgacgact gaagcaggaa gttgcgatag tggtcgcgcc ggtgctcgtc
      661 cgcggccagg gcgtcgagaa acgggtcgga cgcgacggcc cgcagttcgg gcagcaggcg
      721 atcgacatag gccagggcgt cgccgtcgcc ggccgcggcg ttcgcctcgt cccatagcgg
      781 cccgatcaac tcggcctcga ggcgcggcct cgcggcgttc agcgccgcca tgcgatcctc
      841 gccatgatgg cggtcaagcc gctcgagaag gtcgccatcc ggcgccgtct ccggcagcac
      901 cacatagcgc gcctggtagc agcgcagcag cgcctggtgc cggatgtgcg ccgcgagggc
      961 cgggtcggcg tcgtctggca ggggatcgat ccaggtgtca aaggccggcg gcgggttcgg
     1021 tcccgccgcg tcggcgaggc atgcgccgag cgcgttgagc gcctcgatcg tgaccggctc
     1081 aagcatcggc gggccggtag accgacaccc cgacgccgcc cttgatgaac agatcctcga
     1141 gccgccagcg cgacagggag atcgtcgcgc cgttgaggta ggtcggggcg tccggcgcgc
     1201 cggcgatgac cgggatatgc ttgatgatga tccgcgagac gagctcggcc ttgaacaggc
     1261 tggacagcag ctcgctgccc ccctcggcga tcatcgtctt caccccgcgg ccgtccagct
     1321 gatccatcag ggcttcaaga tcgacctggt cttcgccgca gacgacgacc tcgacatgat
     1381 cgtgcgagcg cagggccgcg accgcctcgg gcgtcgcccg ccgcgacacc gcgaccagcg
     1441 tcggcacgcc gtcgttgagc agggtgcagc cgggtggcag ccgaccatcc gatgacggga
     1501 tgacccgcag tggattacgg ccgggcgcgt ggcgaacggt cagttccgga tcgtcgatcc
     1561 gcacggtatt ggccccgacc atgatggcgt cgttggccgc ccgctggcca tggaaccagg
     1621 ccttcagctc gtcactcgaa tagaggaaca gatccttgct ggaggcggat ttgcccagcg
     1681 acagcttgcc gtcgatggtc agctcggaca gcgtcgtgat ctcgaccatg gtccccccgt
     1741 cgacatcgca cgatgtcatc ttgccgcgtt ttcgcccgtt cgagaacgcc gggccgacga
     1801 ttgggttcaa acagatgcag gccaggactg gtcaggtccg gcgaggcccc ttatctgcgg
     1861 aaccgcgaca ggggagcttg cgtgcagcca atcatttcca tcgccggtct ttccaagacc
     1921 tacgccggcg gattccaggc cctcaagggc gtcgatctcg acatccgccc gggggagatc
     1981 ttcgccctgc tgggacccaa cggggccggc aagacgacgc tgatcggggt ggtctgcggc
     2041 attgtcaacg ccaccgccgg caccgtcatc gcggacggtc atgacatcgt tcgcgactac
     2101 cgcgcggccc gcaccaagat cgggctggtg ccgcaggaac tgtcgaccga cgcgttcgag
     2161 acggtcatcg ccacggtcaa gttcagccga ggcctgttcg gcaagccgcc caatccggcc
     2221 tacatcgaga agatccttcg cgacctcagc ctgtgggaca agaaggacaa caggatcatg
     2281 accctgtccg gcggcatgaa gcgccgggtg atgatcgcca aggcgctcag ccacgagccg
     2341 aagatcctgt tcctcgacga gccctcggca ggggtcgacg tcgagctgcg ccgcgacatg
     2401 tggcagatgg tccgcgccct gcgcgacagc ggcgtgacca tcatcctgac cacccactac
     2461 atcgaggagg ccgaggagat ggccgaccgg atcggggtga tcaacaaggg cgagctgatc
     2521 ctggtcgagg acaaggacgt gctgaggcgc aagctcggca agaagcagct gacgatccag
     2581 ctgcgcgagc cgctgacggc cctgccggcc ggtctggcga acgacgcgct cagcctttcg
     2641 gcggacgggc tggacctgac ctggagcttc gacgcccagg ccgaggaaac cggcatcgcc
     2701 gccctgctca agcggctggg cgagcacggc atcgacttca aggatctgcg aaccgaggaa
     2761 agctcgctgg aagagatctt cgtcaacctg gtgagggcgg gatgatcaac cttcatggcg
     2821 tgaaggccat ctaccgcttc gaactggccc gctggttccg caccctgatg cagtcgatcc
     2881 tggcgccggt catctcgacc tcgctgtatt tcgtcgtctt cggctcggcc atcggcagcc
     2941 ggatgaccac catcgacgga gtcagctacg gcgccttcat cgtgccgggt ctgatcatgc
     3001 tgtcgatcct gaccgagagc atctccaacg ccagtttcgg catctacatg cccaaatggt
     3061 cggggacgat ctacgagttg ctgtcatcgc cggtcagctg gatcgagacg gtcatgggct
     3121 acgtcggggc ggcggcgacc aagtcggtgc tgctcggcct tttgatcctg gtcacggcgc
     3181 ggctgttcgt gccgttcgag attgtccatc ccgtctggat gctgggcttc ctcgtgctca
     3241 ccgccgtcac cttcagcctg ttcggcttca tcatcggcct gtgggccgat gacttccaga
     3301 agctgcagat cgtgccgacc ctgatcgtta cgccgctgac cttcctgggc ggcagcttct
     3361 attcgatcag catgctgccg ccggtctggc agaccgtcac cctgttcaac ccggtggtct
     3421 atctgatcag cggcttccgc tgggccttct acggcaaggc cgacgtcaac gtcggcgtca
     3481 gcgtcgccgc cacggtcggc ttcatgatcg cctgcctgat cgccatccgc tggttcttcc
     3541 gcaccggcta caagatgaaa gcctgacctc gcatgtcctc tgacctgttc gccccgctga
     3601 ccttcgccca cggcccggcg atgaagaacc gcctgatgct ggcgccgctg accaacaccc
     3661 agagccatgc ggacggccgc atgtcggacg aggagttccg ctggctgacc atgcgcgccg
     3721 agggcggctt cggcctgacc atgacctgcg cctcgcacgt gcagcgcaac ggccagggct
     3781 ttccgggcca gatgggttgc tggtcggacg accatatcga ggggctgacc cgcctggccg
     3841 cggcgatcaa ggcgaacgac agcctggccg tggtccagat ccaccacgcc ggcatgcgct
     3901 cgccggccga gctgatcggc gaggcgccgg tctgcccgtc cgacaacgac gagttcaagg
     3961 cccgcgccct gacggccggg gaggtcgaac agctgcgcga tgacttcatc gccgccgccg
     4021 tgcgcgcgaa gacggccggc ttcgacggcg tcgagctgca cggggcccac ggctacatca
     4081 tcggccagtt tctctcgccc gagatcaacc tgcgcacgga ccaatggggc ggcagccccg
     4141 agaaccgcgc ccgcctggcg atcgagatcc tccagggcat ccgcgcgcaa tgcggcgagg
     4201 gcttccagat cggcctgcgc ctgtcgcccg agcggtt
//
LOCUS       DAORNX010000260         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15578_length_4234_cov_7.964824, whole genome shotgun sequence.
ACCESSION   DAORNX010000260 DAORNX010000000
VERSION     DAORNX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15578_length_4234_cov_7.964824"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PLO65_11320"
     CDS             <1..152
                     /locus_tag="PLO65_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28880.1"
                     /translation="LTARGWTRAIRLARTIADLEGAGSVGRIHVAEALIYRRVGAEAG
                     ANAGA"
     gene            223..1755
                     /locus_tag="PLO65_11325"
     CDS             223..1755
                     /locus_tag="PLO65_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD28881.1"
                     /translation="MAPRQAVLGMEQASFHHGAKPVFENVSFLLDDARTALVGENGAG
                     KSTLLKCLAGELELDRGQVLKSRGLRVASLPQDTPAALADLSVREALRLSLDRIGAGD
                     DDWRIDVLLDEIGVSFETAEQRFGELSGGWQRLLLIAGAARLEEPDILVLDEPTNHLD
                     LANIGILERWLTESFALPMLIVSHDRAFLDRVTDRTLFLRSDGMHAFKSRFSVAREEL
                     LRRDAAAAAHRRLEEKEVKRLEAAAARYKVWAVKNPELNKRKAAIETRIARLEADRTQ
                     TFVARDRRLELSDGEIEAKVALRLTGLMVKTPDAGRTLLTIDRLTVAAGDRIAILGAN
                     GAGKSTLLTALAAAFDPELEHYDGQAAVRFNPATRLAYFDQAMRGLPLGSSLLDYVAG
                     APDATEKEAVRLLAQAGFPFVRIGDPIALLSHGERSRLMFLRLKLERPNLYLLDEPTN
                     HLDIEGQEALEAELEEAGVSCLFVSHDRYFTRTAATRFLEIRKGRLVEVEGPDAFFDA
                     QG"
     gene            1985..3109
                     /locus_tag="PLO65_11330"
     CDS             1985..3109
                     /locus_tag="PLO65_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRD28882.1"
                     /translation="MSTAVQLLVGTRKGAWIYRSDAARETWTVDGPHFLGNIVNHLVL
                     DPRDGRTLLMAARTGHLGPTIFRSTDGGASWTEASRPPAFPKSADGEPARAVDHSFWV
                     EPGHADEPGVWWAGTSPPGLFVSRDNGDSWEGVSGFNDHPMYWKWCPADGGTPDGALL
                     NQIVIDPRDPAHMYIATSTGGVFESLDRGGSWTPLNRNVEANFFPDPYPEYGQDAHYI
                     ALAPTMPDRLWQQNHCGVYRLDRPSETWERIGKAMPAGIGDIGFTITPHPRDADTAWV
                     FPMDGTEVWPRTSPGGRPAVYRTRDAGASWERQDRGFPREQGWFTVKRQAFCADRADP
                     LGLYLGATNGELWASINEGGDWRCIARHLPEIYSVVAAPL"
     gene            3109..3372
                     /locus_tag="PLO65_11335"
     CDS             3109..3372
                     /locus_tag="PLO65_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRD28883.1"
                     /translation="MARVFIPSQLQSYSGGLSLVEAPGATVEAVLRALDSRFPGIRFR
                     VIDEQDRVRPHMRLFVGSERTLDIRDPLGESDELLIFGALSGG"
     gene            3377..3754
                     /locus_tag="PLO65_11340"
     CDS             3377..3754
                     /locus_tag="PLO65_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28884.1"
                     /translation="MRIWKLVFMVAALFNFAVGLPMLLAPEAMLAQLGQPVPDDLLMT
                     RLASLLIVVLGIGYGMVARDPAANRPILWLGVLGKAPIPLLVWLSGGAAALASSAFML
                     SLGDLAFAALFLVFLARTPKSAA"
     gene            3790..>4234
                     /locus_tag="PLO65_11345"
     CDS             3790..>4234
                     /locus_tag="PLO65_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRD28885.1"
                     /translation="MPDNRAITPEQLATLSHEFRTPLNGVLGMARLLDGTRLTAEQKS
                     YVAALRESGDHLLALVNDVLDLARLGAGKIELHPAPTDIAALLRQVAELMSPRAHEKG
                     IEIAWACDGALPPVMADEGRLRQVLLNFAGNAVKFTESGGVLLTAG"
BASE COUNT          612 a         1497 c         1494 g          631 t
ORIGIN      
        1 acctgaccgc gcgcggctgg acccgggcca ttcgcctggc ccgcaccatc gccgatctcg
       61 agggcgcggg cagcgtcggt cgcatccatg tcgccgaggc gctgatctat cgtcgggtgg
      121 gcgcggaagc aggggcgaac gccggcgcct gaccgccggt ggcgcgggcc gcccgcgtgg
      181 gttacccaga gcacgcccgt cgcgggcgag ggggatcggg ccatggcgcc acgtcaggcc
      241 gtgctcggca tggaacaggc cagttttcac cacggggcca agcccgtgtt cgagaacgtg
      301 tccttcctgc tcgacgacgc ccgcacggcc ctggtcggcg agaacggggc cggcaagtcg
      361 accctgctca aatgcctggc cggcgagctg gagctggaca gggggcaggt gctgaagtcc
      421 cgcggcctcc gggtcgcttc gctgccgcag gacacgcccg cagccctggc cgacctgagc
      481 gtacgcgagg cgctgcggct gtcgctggac aggatcggcg cgggcgacga cgactggcgc
      541 atcgatgtgc tgctcgatga gatcggcgtg tcgttcgaga cggcggagca gcgcttcggc
      601 gagctgtcgg gcggctggca gcggctgctg ctgatcgccg gggcggcgcg gctggaagag
      661 cccgacatcc tcgtcctcga cgagccgacc aaccacctgg acctggccaa catcggcatc
      721 ctcgagcgat ggctgacgga gtccttcgcg ctgccgatgc tgatcgtcag ccacgaccgc
      781 gcgttcctcg accgcgtcac cgaccgcacg ctgttcttgc ggtccgacgg catgcacgcc
      841 ttcaagtcgc gcttttcggt ggcccgcgaa gagttgctgc gccgcgacgc ggcggcggcc
      901 gcccaccgca ggctggagga gaaggaggtc aaacggctgg aggcggcggc ggcccggtac
      961 aaggtctggg cggtcaagaa ccccgagctg aacaagcgca aggcggcgat cgagacgcgc
     1021 atcgccaggc tggaggcgga ccgcacccag accttcgtcg cccgtgaccg gcggctggaa
     1081 ctgtccgatg gcgagatcga ggcaaaggtc gcgctgcggc tgacgggcct gatggtgaag
     1141 acgcccgacg ccggccgcac cctgctgacc atcgaccggc tgaccgtcgc ggccggcgac
     1201 cgcatcgcca tcctgggggc caacggggcg ggcaagtcga cgctgctgac ggcgctggct
     1261 gcggccttcg atcccgagct cgaacactat gacggccagg ccgccgtgcg cttcaacccc
     1321 gcgacccggc tggcgtactt cgaccaggcg atgcgaggtc tgccattggg atcgagtctg
     1381 ctggactacg tcgcgggcgc ccccgacgcg acggagaagg aggctgtgcg gctgctggcc
     1441 caggccggct ttccgttcgt gcgcatcggc gacccgatcg ccctcctcag tcatggcgag
     1501 cggtcgcggc tgatgttcct gcggctgaag ctcgagcggc ccaacctcta cctgctcgac
     1561 gaaccgacca accatctcga catcgagggc caggaggcgc tggaagcgga gctggaagag
     1621 gcgggggtgt cctgcctgtt cgtgtcccac gaccgctact tcacccgcac ggccgcgacc
     1681 cgcttcctcg agatccgcaa gggccggctg gtcgaggtgg agggcccgga cgccttcttc
     1741 gacgcgcagg ggtaggggcc cggcagcgga cggcctcacg ggtggcggac cggccttgat
     1801 gtcttgatga gcattttggc tccggcggaa ccttgcccaa ggaccgcctt cggcggccgc
     1861 gcagcggcgg gccctggacg ggccgtcctt gaccaggggc ccgaccggag cgaaccattc
     1921 catcaagacc ttcaggggcc cctgtcatcg ggtcgctgca ggcaggcgga gttggaggga
     1981 cgccatgtcc accgcggttc agcttctcgt cggcacgcgc aagggcgcct ggatctaccg
     2041 cagcgacgcc gcccgggaga cctggacggt cgacgggccg catttcctcg gcaacatcgt
     2101 caaccatctg gtgctcgatc cgcgtgacgg ccgaacgctg ttgatggcgg ccaggaccgg
     2161 ccacctcggc ccgacgatct tccgctccac cgacggcggc gcgagctgga cggaagcctc
     2221 gcgcccgccg gccttcccga agtccgccga cggcgaaccg gcaagggccg tcgaccacag
     2281 cttctgggtc gagccgggcc atgccgacga gcccggcgtc tggtgggccg ggacctcgcc
     2341 gcccggcctg ttcgtcagcc gcgacaacgg cgacagctgg gagggtgttt ccggcttcaa
     2401 cgatcacccg atgtactgga aatggtgtcc cgcggacggc ggcacgcccg acggggccct
     2461 gctcaaccag atcgtcatcg acccgcgcga cccggcccac atgtacatcg ccacctcgac
     2521 cggcggggtg ttcgagagcc tcgaccgcgg tggctcctgg acgccgctga acaggaacgt
     2581 ggaggcgaac ttcttccccg atccatatcc ggagtacggc caggacgccc actacatcgc
     2641 cctggccccg accatgcccg acaggctctg gcagcagaac cactgcggcg tctaccgcct
     2701 cgaccggcca tcggagacct gggagcggat cggcaaggcc atgccggccg ggatcggcga
     2761 catcggcttc accatcaccc cgcacccgcg cgacgcagac acggcctggg tgttcccgat
     2821 ggacggcacc gaggtctggc cgcgcacgag tccgggcggg cggcccgccg tctaccgcac
     2881 ccgcgacgcc ggagcgagct gggagcggca ggaccgagga ttcccgcgcg agcagggctg
     2941 gttcaccgtc aagcgccagg ccttctgcgc cgaccgcgcc gatccgctgg gcctgtatct
     3001 gggcgcgacc aatggcgagc tgtgggccag tatcaacgag ggcggcgact ggcgctgcat
     3061 cgcccgccat ctgccggaaa tctattcggt ggtcgcggcg ccgctctgat ggcccgggtc
     3121 ttcatcccct cgcagctgca gtcctattcg ggtggcctgt ccctggtgga ggctcccggc
     3181 gccacggtcg aggcggtgct gcgggcgctc gacagccggt ttcccggcat ccgcttcagg
     3241 gtcatcgacg agcaggaccg ggtgcggccg cacatgcggc tgttcgtcgg ctccgagcgc
     3301 acgctcgaca tccgcgaccc tcttggcgaa agtgacgagt tgctgatttt cggcgcgctc
     3361 agcgggggct agggccatgc ggatctggaa gctggtgttc atggtcgcgg cgctgttcaa
     3421 cttcgccgtc ggcctgccga tgctgctggc gccggaggcg atgctggccc aactgggtca
     3481 gccggtcccc gatgacctgc tgatgacccg gctggcctcg ctgctgatcg tggtgctggg
     3541 gatcggctac ggtatggtcg cccgtgaccc cgccgccaac cggccgattc tctggctggg
     3601 cgtgctcggc aaggcgccga tccccctgct ggtctggctc agcggcggcg cggcggccct
     3661 ggccagcagc gccttcatgc tgtcgctcgg cgacctggcc ttcgccgccc tgttcctggt
     3721 cttcctcgcc cggacgccga agtccgccgc ttgaccgccc gttaagccgc gccggctagt
     3781 ctggcgccca tgccggacaa ccgcgccatc acgcctgagc agctggccac cctgagccac
     3841 gagttccgca cgccgctgaa cggcgtgctg ggcatggcgc ggctgctcga cggcacccgg
     3901 ctcaccgccg agcagaaatc ctatgtcgcc gccctgcgcg agagcggcga ccacctgctg
     3961 gccctggtca acgatgtgct cgacctggcc cggctggggg ccggcaagat cgagctgcat
     4021 cccgcgccga ccgacatcgc cgccctgctg cggcaggtcg ccgagctgat gagcccgcgg
     4081 gcccacgaga aggggatcga gatcgcctgg gcctgtgacg gcgccctgcc gccggtgatg
     4141 gccgacgagg gccggctgcg ccaggtgctg ctcaacttcg ccggcaacgc ggtcaagttc
     4201 accgagtccg ggggcgtgct gctgaccgcc gggc
//
LOCUS       DAORNX010000261         4226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15623_length_4226_cov_9.171901, whole genome shotgun sequence.
ACCESSION   DAORNX010000261 DAORNX010000000
VERSION     DAORNX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15623_length_4226_cov_9.171901"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLO65_11350"
     CDS             complement(<1..390)
                     /locus_tag="PLO65_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRD28886.1"
                     /translation="MRHHLFAVCAATLLSTAYAAEPFALKSTDFAHKGKLSDKHVFAG
                     FGCTGGNTSPSLAWSNPPAGTRSFALMVHDPDAPTGGSGWWHWVVLDIPATARQLPAG
                     AGKADGSALPAGASQWKTDFGGPGWGGP"
     gene            complement(348..815)
                     /locus_tag="PLO65_11355"
     CDS             complement(348..815)
                     /locus_tag="PLO65_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28887.1"
                     /translation="MFVLVHVSSSVPDLDQASPFYDAVMAALGVAQFYDRPDAIGYGV
                     RYDSIEDFHSCLAIHASANANVDDKRHWCFKATTRQQLIEFHRAGLADCGRDNGAPAL
                     RHYHPTYFGAFLHDPFGNRIEAVGYRAELNLPQPSKGKEACDTTCSPFAPPRC"
     gene            complement(854..1726)
                     /locus_tag="PLO65_11360"
     CDS             complement(854..1726)
                     /locus_tag="PLO65_11360"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28888.1"
                     /translation="METLGFIGTGVMGFPMARNLARHFGQLVVWNRSPGKSAALVPFG
                     AQVAASPGELLDRCRIVIMMLATEDAIDDVLRQAGADLPGSLSGRIVVHMGTTSPAYS
                     AALNERIRPAGGQYVEAPVSGSRLPAENGELVGMLAGDPDAVAEVRPWLAPVCKETFV
                     CGPVPSALQMKLAVNLFLITLVSGLVESHHFAREIGLDTALHRAILDAGPMASAVSKM
                     KLEKLVADDFSVQASIADVQKNSRLVAEEARRGGIVSPLIEECYRLFSETLAGGHGGL
                     DMIGVLRAIEGRKR"
     gene            complement(1839..2762)
                     /locus_tag="PLO65_11365"
     CDS             complement(1839..2762)
                     /locus_tag="PLO65_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD28889.1"
                     /translation="MTLMDPHGYGSNNQRIPPITGLMAFESVARNGSFSKAADELSIT
                     QSAVSHRISQLESLLGASLLIRIGHSVSLAPAGKELLPYVREGLGCLRDGLAKISTEG
                     KPSLKLSLAPAVAANWLIQRLSGFPRKHPDINLEINVTSKNINLRSGEADVAIRCGDG
                     RWDGLDAIELIPLNAVAVCSPAYKKAHPWLRRLEDLERATLLRHNFISWKDWFARLGT
                     EVKLPASGLSFSEVSLLITAAECSQGVALVFDALVERHIQDGTLVQAVPQSLVSERSY
                     YIITPTGAPRSDALQAFIDWLLTLKPEPAAR"
     gene            complement(2759..3712)
                     /locus_tag="PLO65_11370"
     CDS             complement(2759..3712)
                     /locus_tag="PLO65_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD28890.1"
                     /translation="MRPAFLRRLTGNDALAGIAAGLAVAVITAIWSVATRFAVTTSLP
                     PHDVTLIRFSVAAFFLWPVFLGSLRRYRQTRPAALLLMLGGAGVPFMWTAATGLTCAP
                     VTHIATLMIVMMPIFVTLISWLVFRETLAGRQLAGVATVVAGVVLISGSSLFLNRQAG
                     EWVGDLMFLGASCLFATFTVTQRRSGLTPWQATALVNVGSTILFLPVHLALPNSGIPN
                     ASLQELAIQALAQGIGMALLGLYFYTVAVGRLGAQRAAIFLALVPPFSTVLGMVFLDE
                     QPSAIALGGIVLVMVGVFAVVFCARQNGNGMVESALQRRHG"
     gene            complement(3775..>4226)
                     /locus_tag="PLO65_11375"
     CDS             complement(3775..>4226)
                     /locus_tag="PLO65_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28891.1"
                     /translation="RVCSGNQPLTERGRHMPCASIILCGKKPDPATIAALHQEMATII
                     ERDLEATKALIAVRIVHDQPGTWSVAGKPPDAAVEGTGAYIDIHVAEDAVDEARMAAA
                     MKAIQECTTTLLGKNVLPPYVVFTRVPIAAWGFKGKSIQQIRAAMAA"
BASE COUNT          693 a         1225 c         1625 g          683 t
ORIGIN      
        1 ggggccgccc cagccggggc cgccgaagtc ggtcttccac tggctggcac cggccggcag
       61 cgccgagcca tcggccttgc cggcaccggc gggcagttgc cgggcggtgg cggggatgtc
      121 gaggaccacc cagtgccacc agccgctgcc gccggtcggg gcgtcggggt catgcaccat
      181 cagcgcgaag ctcctggtgc cggccggcgg gttgctccag gcaagggacg gtgaggtgtt
      241 gccgccggtg cagccaaagc cggcgaagac gtgcttgtcg ctcagcttgc ccttgtgggc
      301 gaagtcggtg ctcttgagcg cgaatggctc ggcggcgtac gcggtgctca gcagcgtggc
      361 ggcgcaaacg gcgaacaggt ggtgtcgcat gcttcttttc ctttgctggg ttgggggagg
      421 tttaattctg cccgatagcc gaccgcttcg atgcggttgc caaacgggtc gtggaggaag
      481 gcgccgaagt aggtggggtg gtagtggcgc agggcgggcg cgccgttgtc gcggccgcag
      541 tcggccagcc cggcgcggtg gaattcgatg agctgttggc gggtggtggc cttgaagcac
      601 cagtggcgct tgtcgtcgac attggcgttg gccgaggcat ggatcgccag gcagctgtgg
      661 aaatcctcga tgctgtcgta gcggacgccg tagccgatgg catccgggcg gtcgtaaaac
      721 tgcgcgacgc ccagcgcggc catcaccgca tcgtagaagg gactcgcctg gtcgaggtcg
      781 ggaacggagc tggaaacgtg gacgagtacg aacatggggg cctcgggaaa ggatggcgga
      841 tgacggaatt cgttcagcgc ttgcgcccct cgatggcgcg cagcacgccg atcatgtcca
      901 gcccgccgtg gccgccggcc agggtttcgc tgaacagccg gtagcattcc tcgatcagcg
      961 ggctgacgat gccgccgcgc cgggcttcct cggcgacgag gcggctgttc ttctgcacgt
     1021 cggcaatcga ggcctgcact gagaaatcgt cggcgacgag cttttccagc ttcattttcg
     1081 acaccgcgct ggccatcggg ccggcatcga ggatggcacg gtgcagcgcg gtgtcgaggc
     1141 cgatttcgcg agcgaagtgg tgcgactcga caagaccgct gaccagggtg atcaggaaga
     1201 gattgaccgc cagcttcatc tgcagcgccg acggcaccgg gccgcagacg aaggtttcct
     1261 tgcacaccgg ggcgagccac gggcgcacct cggccacggc gtcggggtcg ccggccagca
     1321 tgccgaccag ctcgccgttt tcggcgggca ggcgggagcc ggagacgggc gcctcgacat
     1381 actggccgcc ggcggggcgg atgcgttcgt tcagtgcggc ggaataggcc ggcgacgtcg
     1441 tacccatgtg caccacgatg cggccgctca gcgagccggg gaggtcggcg ccggcctggc
     1501 gcaggacgtc gtcgatggcg tcttcggtcg ccagcatcat gatcacgatg cggcagcgat
     1561 caaggagttc gccggggctg gccgcgacct gcgcgccaaa cgggacgagc gccgccgatt
     1621 tcccgggcga gcgattccag acgacgagtt gcccgaagtg ccgggccaga ttgcgcgcca
     1681 tgggaaagcc catgacgccg gtgccgatga agccgagcgt ttccattttt ccacctcgcg
     1741 atgtgtgcat tggtcaggcc atcatagggg cgtggaaaat cacggcgatg cgacttgctc
     1801 atgggccgga tcgaaatcct tcatggcccg gccgggtgtc agcgtgcggc gggttccggc
     1861 ttcagggtga gcagccagtc gatgaaggct tgcagcgcgt cgctacgcgg ggcgccggtg
     1921 ggcgtgatga tgtagtagga gcgttccgag acgaggcttt gcggcacggc ctggacgagt
     1981 gtgccgtcct ggatatggcg ctcgacgagg gcgtcgaaaa cgagggcaac gccctgcgag
     2041 cattcggcgg cggtgatgag cagcgagact tcgctgaacg acaggccgga ggcggggagc
     2101 ttgacctcgg tgccgagccg ggcgaaccag tccttccagg agatgaagtt gtggcgcagc
     2161 aacgtggcgc gttcgaggtc ttcgaggcgc cgcagccacg ggtgcgcctt tttgtaggcc
     2221 gggctgcata cggcgactgc gttcagcggg atcagttcga tggcgtcgag gccgtcccag
     2281 cgcccgtcac cgcagcggat cgcgacatcg gcctcgccgg agcgcagatt gatgtttttg
     2341 gacgtgacgt tgatttccag gttgatgtcc ggatgcttgc gggggaagcc ggacaggcgc
     2401 tggatcagcc agttggcggc gacggcgggg gcgaggctga gcttgaggct gggcttgccc
     2461 tcggtggaaa ttttcgccag cccgtcgcgc aggcagccca gcccttcgcg gacgtagggc
     2521 agcagttcct tgccggcggg tgccaggctg accgagtggc cgatgcggat gagcagcgag
     2581 gcgccgagca gggattcgag ctggctgatg cggtggctga cggcgctctg ggtgatcgac
     2641 agttcgtcgg cggccttgga gaagctgccg ttgcgggcca ccgattcgaa ggccatcagg
     2701 ccggtgatcg ggggaatgcg ctggttgttg ctgccgtatc cgtgggggtc catgagggtc
     2761 atccgtgtcg ccgctggagc gcagactcta ccatgccatt gccgttctgc cgggcacaga
     2821 agaccacggc gaagacgccg accatgacca gaacgatgcc gcccagggcg atcgccgaag
     2881 gctgctcgtc gagaaagacc atgccgagga cggtggagaa gggtggcacc agcgccagga
     2941 agatggctgc acgctgcgcg ccgaggcggc cgacggcgac ggtgtagaaa tagaggccga
     3001 gcagggccat gccgatgccc tgggcgaggg cctggatggc gagttcctgc aggctggcgt
     3061 tggggatgcc cgagttcggc aacgccagat ggacgggcag gaatagaatg gtcgagccga
     3121 cattgaccag cgcggtggcc tgccaggggg tgaggcctga gcggcgctgg gtgacggtga
     3181 aggtggcgaa caggcagctt gcgccgagga acatcaggtc gccgacccat tcgccggcct
     3241 gccggttgag gaagagggaa ctgccggaga tcagcacgac accggccacg acggtggcga
     3301 ccccggccag ctgccgtccg gccagtgttt cgcggaagac cagccaggaa atgagggtca
     3361 cgaagatcgg catcatgacg atcatcagcg tggcgatgtg ggtgaccgga gcgcaggtca
     3421 ggccggtggc ggcggtccac atgaagggca cgccggcgcc gccgagcatg agcaggaggg
     3481 cggcgggccg cgtctgccgg tagcggcgca ggctgccgag aaagaccggc cacaggaaga
     3541 aggcggccac cgagaagcgg atgagcgtga cgtcgtgcgg cggcagcgag gtggtgaccg
     3601 cgaagcgggt ggcgaccgac cagatggcgg tgatgacggc gacggccagg ccggccgcga
     3661 tgccggcgag ggcgtcgttg ccggtgaggc ggcgcaggaa ggcagggcgc atgggcggat
     3721 tctggagagt gggcgtgccg gccagcggag gcgtctggct ggccggggag tcgatcaggc
     3781 cgccatcgcc gcgcggatct gctggatgga cttgcccttg aagccccagg ccgcgatggg
     3841 cacgcgggtg aacaccacgt agggcggcag gacgttcttg ccgagcagcg tggtggtgca
     3901 ttcctggatg gccttcatgg cggccgccat cctggcttca tcgacggcat cctcggcgac
     3961 gtggatgtcg atgtaggcgc cggtgccttc gacggccgca tcgggcggct tgccggcaac
     4021 cgaccaggtg ccgggttggt catgcacgat gcgaacggcg atcagggcct tggtcgcctc
     4081 caggtcgcgt tcgatgatag tcgccatttc ctggtgcagg gcggcaatgg tggctgggtc
     4141 gggcttcttg ccgcaaagga tgatgcttgc gcagggcatg tgtctacctc gttctgtcaa
     4201 gggctgattc cctgagcaga ctctag
//
LOCUS       DAORNX010000262         4182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15861_length_4182_cov_7.967773, whole genome shotgun sequence.
ACCESSION   DAORNX010000262 DAORNX010000000
VERSION     DAORNX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15861_length_4182_cov_7.967773"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLO65_11380"
     CDS             <1..802
                     /locus_tag="PLO65_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRD28892.1"
                     /translation="GSRAEFAARKTTRVIDGQSITGWFTEDFTLAEIKTLRARERLPQ
                     LRPANTAYDGQEAIPTFEEVVDIARAAGVGVYPEMKHPRHFASIGLPFEQRMADALKA
                     ANLNSRTASVFVQCFEVGPLKTLRALTKARLVQLVDSEGAPADRPDLRYGDMVTAEGL
                     RAIAAYADGVGPDKSYVVPTDGQTLRPPTDLVTNAHAAGLQVHPWTVRAENYFLPVSL
                     QRGAADSTRPRQHGDVGAVFKALYDAGVDGLFSDFPGLAVAARKEFVA"
     gene            799..2085
                     /locus_tag="PLO65_11385"
     CDS             799..2085
                     /locus_tag="PLO65_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28893.1"
                     /translation="MIRIALAAVAACFLFAAAPAQARSPLDPLAERYVKLSLEIGEKE
                     DGYIDAYYGPPEWQAAAKVAPRDLPALRAEVASLSSAVAHIKPGWLVGDEQRRRLFLL
                     AQLRAADTRLRMMGGEKLSFEDEAEGLFGVRPELKPLSAYDPVLREIEALVPGEGPLW
                     QRVDSFSDRFIVPADRLRAVMDAAIAECRKRTMAHIALPTDERFTLEFVTGKSWSGYN
                     WYKGDATSLIQVNTDLPVRISRAVDLGCHEGYPGHHVLNMLLEERLSKGKGWVEFTVY
                     PLFSPQSLIAEGSANYGIELAFPGAEKAAFEAATLYPLAGLDPKDAARYDALLEAQKA
                     LAGARFTIASDYLSGKITKDQAIALTQTYGLVSAKRAAQTIAFTDQYRSYVINYGLGL
                     DMVRAYVEGAGADQDARWKAMEWVISGPTLPGDLLR"
     gene            complement(2206..2718)
                     /locus_tag="PLO65_11390"
     CDS             complement(2206..2718)
                     /locus_tag="PLO65_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRD28894.1"
                     /translation="MAYFSTKTYGGERGLSCAMRAWTAESHCNLLHGYSLGFRFTFAA
                     EQLDHRGWVVDFGKGGFGPIREWLHATFDHTTLVAKDDPHFAEFVRLRDLGLCDMRVL
                     PAVSCERIARFVFDHAQPMIEAATRGRCWIVSVECFEHGANSAIYQNPETILRQVSAE
                     ALAAIVARDE"
     gene            complement(2792..>4182)
                     /locus_tag="PLO65_11395"
     CDS             complement(2792..>4182)
                     /locus_tag="PLO65_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD28895.1"
                     /translation="RMTTDVTIIENLVGSAASIALRNILGLIGATVMLAFVSPKLTLF
                     VMLGAPLLIVPLFLFGRRVRSLSTKTQDRFAQAVGFAGESLDALDTVQAFGREAMADK
                     RFGDAVETAFRTSLSRVFARSLMTLVVIGLVFGGIGLLLWTAAHAVVNHTMSPGQLIQ
                     FLALSVFAGGSVGALGEVWGVVQQASGAMQRIFELLASKTLIAAPAHPRPLPVPARGE
                     ITFDNVTFAYPGRPDLPALHGFDLQVKPGETVALVGPSGAGKSTVLRLLLRFYDPQVG
                     RVLLDDVDLRDADPAEVRARMALVSQDASLFSGSALDNIRFGREDATLEEIQAAARAA
                     EADGFIRALPEGYDTPVGERAKTLSGGQKQRLAIARSLVRNAPILLLDEATSALDAEN
                     ERLVQKALEGAMGDRTTLVIAHRLATVLRADRIVVMDEGRVVEQGAHAQLSKAGGLYA
                     NLARLQFGVEAA"
BASE COUNT          660 a         1448 c         1471 g          603 t
ORIGIN      
        1 cggctcgcgc gccgaattcg cggcccgcaa gacgaccagg gtcatcgacg gccagtccat
       61 cacgggctgg ttcaccgagg acttcaccct ggccgagatc aagaccctgc gggcgcgcga
      121 gcgcctgccg cagctgcgcc cggccaacac ggcctatgac ggccaggagg cgatcccgac
      181 cttcgaggag gtggtcgaca tcgcgcgggc ggccggcgtc ggcgtctatc cggagatgaa
      241 gcacccgcgc catttcgctt ccatcggcct cccgttcgag cagcgcatgg ccgacgccct
      301 gaaggccgcc aatctcaatt cccgcaccgc cagcgtgttc gtgcagtgct tcgaggtcgg
      361 accgctcaag accttgcgcg ccctgacgaa ggcgaggctg gtccagctgg tcgattccga
      421 gggcgcgccc gccgacaggc cggacctccg gtacggcgac atggtcacgg ccgagggcct
      481 cagggccatc gccgcctatg ccgacggcgt gggcccggac aagtcatacg tcgtgcccac
      541 cgacggccag accctgcggc cgccgaccga tctggtgacc aacgcccacg ccgccgggct
      601 gcaggtccat ccctggaccg tgcgggcgga gaactatttc ctgccggtca gcctgcagcg
      661 cggcgccgcc gattcaaccc ggccgcgcca gcacggcgat gtcggcgccg tcttcaaggc
      721 gctatatgac gccggggtcg acggtctgtt cagtgatttc ccgggcctcg ccgtcgccgc
      781 ccggaaggag tttgtcgcat gatccgtatc gcccttgccg ccgtcgccgc ctgcttcctg
      841 ttcgccgccg caccggccca ggccaggtcg ccgctcgacc cgctggccga acgctatgtg
      901 aagctgagcc tcgagatcgg cgagaaggaa gacggctaca tcgacgccta ctacggtccg
      961 cccgaatggc aggccgccgc gaaggtcgcg ccgcgcgacc tgccggcgct gcgcgcggag
     1021 gtggccagcc tctcttccgc ggtcgcccac atcaagcccg gctggctggt gggcgacgag
     1081 cagcgccgcc ggctgttcct gctggcccag ctgagggccg ccgacacccg cctgcgcatg
     1141 atgggtggcg aaaagctcag tttcgaggac gaggccgagg ggctgttcgg cgtgcggccg
     1201 gagctcaagc cactgtcggc ctacgacccg gtgctcaggg agatcgaagc cctggtcccc
     1261 ggtgaggggc cgctgtggca gcgggtcgac agcttcagcg accgcttcat cgtgccggcc
     1321 gacaggctgc gggcggtcat ggacgcggcc atcgccgagt gccgcaaacg cacgatggcc
     1381 cacatcgccc tgccgaccga cgagcggttc acgctcgagt tcgtcaccgg caagtcctgg
     1441 tcgggctaca actggtacaa gggcgacgcg accagtctga tccaggtgaa caccgacctg
     1501 ccggtccgca tcagccgggc ggtcgacctg ggctgccacg agggctaccc cggccaccat
     1561 gtgctgaaca tgctgctcga ggagcggctc agcaaaggca agggctgggt cgagttcacc
     1621 gtctatccgc tgttctcgcc ccagtcgctg atcgccgagg gcagcgccaa ctacggcatc
     1681 gagctggcct tcccgggcgc cgaaaaggcg gccttcgagg cggcgacgct ctatccgctg
     1741 gccgggctcg atccgaagga cgcggcccgc tacgacgccc tgctggaggc gcagaaggcc
     1801 ctggccggcg cccgcttcac catcgccagc gactacctgt cggggaagat caccaaggac
     1861 caggccatcg ccctgaccca gacctacggc ctggtctcgg cgaagcgcgc ggcccagacc
     1921 atcgccttca ccgaccagta ccgaagctat gtgatcaact acggcctggg gctggacatg
     1981 gtgcgggcct atgtcgaggg cgccggtgcg gaccaggacg cccgctggaa ggccatggaa
     2041 tgggtgatca gcgggccgac cctgccgggc gatctgctgc ggtagggggc attgcgtcct
     2101 tcgacacggc ccttcgggcc tgctcaggat gacgtgcgct tgtgtacgtc atggtgagca
     2161 gccccggacc ttctccgggg cgtgtcgaac cacgcaatgc tctccctact cgtcccgcgc
     2221 cacgatcgcc gccagggcct cggcggagac ctgccgcagg atggtctcgg ggttctggta
     2281 gatggcgctg ttggcgccgt gctcgaagca ttcgaccgag acgatccagc agcgccccct
     2341 ggtggccgcc tcaatcatcg gctgcgcgtg atcgaacacg aacctggcga tccgctcgca
     2401 gctgacggcc ggcagcaccc gcatgtcgca caggccaagg tcgcgcagcc gcacgaactc
     2461 cgcaaagtgc gggtcgtcct tcgccaccag ggtggtgtgg tcgaaggtcg cgtgcagcca
     2521 ctcgcggatc ggcccgaagc cgcccttgcc gaagtcgacc acccagccgc gatggtcgag
     2581 ttgttcggcc gcgaaagtga acctgaaacc gagcgaatag ccgtgcagca ggttgcagtg
     2641 gctctccgcc gtccaggccc gcatggcgca ggacaggccg cgttcgccgc cataggtctt
     2701 ggtcgagaag taggccatcg gatcaaacgg tctggagtca ggggcgtcgc ggccgggttt
     2761 acgccccggc ggcgacgggg gccagacctc gctacgccgc ctcgaccccg aactgcagcc
     2821 gcgccaggtt ggcgtagagg ccgccggcct tgctgagctg ggcgtgggcg ccctgttcga
     2881 cgacgcggcc ctcgtccatg accacgatgc ggtcggcgcg caggacggtc gccaggcggt
     2941 gggcgatgac cagggtggtg cggtcgccca tggcgccttc gagggccttc tggaccagcc
     3001 gctcgttctc ggcgtcgagc gcgctggtgg cctcgtcgag cagcaggatc ggggcgttgc
     3061 ggaccagcga gcgggcgatg gccagccgct gcttctggcc gccggagagg gtcttggccc
     3121 gttcgccgac cggggtgtcg tagccctcgg gcagggcccg gatgaagccg tcggcctcgg
     3181 cggcgcgggc ggccgcctgg atctcctcca gcgtggcgtc ctcgcggccg aaacggatgt
     3241 tgtcgagcgc cgaaccggag aacagcgagg cgtcctgcga caccagcgcc atgcgcgcgc
     3301 gaacctcggc ggggtcggcg tcccgtagat cgacgtcgtc gagcagaacg cgcccgacct
     3361 gggggtcgta gaaacgcagc agtagccgca gcacggtgct cttgccggcg cccgacgggc
     3421 cgaccagggc gacggtttcg ccgggcttca cctgcaggtc gaagccgtgc agggccggca
     3481 ggtcgggtcg gccgggatag gcgaaggtga cgttgtcgaa ggtgatctcg ccgcgcgccg
     3541 ggacgggcag gggcctggga tgggcggggg cggcgatcag ggtcttcgac gccagcagtt
     3601 cgaagatccg ctgcatcgcc cctgacgcct gctgcaccac gccccagacc tcgcccaggg
     3661 cgccgacgga cccgccggcg aacaccgaca gggcgagaaa ctggatcagc tggcccggcg
     3721 acatggtgtg attgaccacc gcgtgcgcgg cggtccacag cagcaggccg atgccgccga
     3781 acaccaggcc gatgaccacc agggtcatca gcgagcgggc gaagacgcgg ctcagggagg
     3841 tgcggaaggc ggtctcgacc gcgtcgccga agcgcttgtc ggccatggcc tcgcgaccga
     3901 acgcctggac cgtgtccagc gcgtcgaggc tctcgccggc gaagcccacg gcctgggcga
     3961 accggtcctg ggtcttggtc gacaggctgc gcacccggcg accgaacagg aacagcggca
     4021 cgatgagcag gggcgcgccg agcatgacga acagggttag cttgggcgat acgaacgcca
     4081 acatcaccgt cgcgccgatc aggccgagga tgttgcgcag cgcgatcgag gccgccgagc
     4141 cgacgaggtt ctcgatgatc gtcacgtcgg tggtcatccg cg
//
LOCUS       DAORNX010000263         4176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15891_length_4176_cov_5.860713, whole genome shotgun sequence.
ACCESSION   DAORNX010000263 DAORNX010000000
VERSION     DAORNX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15891_length_4176_cov_5.860713"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..734
                     /locus_tag="PLO65_11400"
     CDS             255..734
                     /locus_tag="PLO65_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HRD28896.1"
                     /translation="MMPRKPFWEIKSLAEMSPAEWESLCDGCGLCCLVRFEDEETGEV
                     IPTRVHCRLFDSRSCTCTDYENRKRQVPDCIKLTPGNIEQLAWMPASCAYRRLDEGKP
                     LPAWHPLITGDPESVHRAGVSIRDQTLNEDLLDEPEDALDYAAWDLNLDKSDEPYEA"
     gene            complement(722..2458)
                     /locus_tag="PLO65_11405"
     CDS             complement(722..2458)
                     /locus_tag="PLO65_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28897.1"
                     /translation="MTRQEDYVDLGAALSMRTRSTVVRLAITAVIGVLLWTMTDWRPA
                     PAWWAAYAVLQLLLIRVSPTGSRPLRLYGLSLLSYAVAGLPAWHLWTHAGDLGVAAGT
                     MYLSGMLVQLIISALGARRLFWASAAPLIAYLVLIPPFAFGAEGLTASACAVLLVGYM
                     TVVWLGQQRAIGALGEARRQAVALQREAEAASQAKTDFLAAMSHELRTPMNAVLGAAD
                     LLGRTDLTEEQRGHVAMLSDGGSILMQVLNDVLDLAKIEAGKLDIDPANADIHDFVRR
                     CAALWAPRAQDKNLVFEKVITPGTPQWVVVDVTRTGQIVFNLISNALKFTEAGRVGLT
                     LAAEETGPGLIDLILTVSDTGIGMSPEVQQRLFNAFEQADGSTSRRFGGTGLGLSISQ
                     RLAGMMGGAIRAQSVEGQGSTFTLRLPCRVGEAVVAAPGSGPVDPDADRPAARILVAE
                     DNPSNQRVIELFLRPLGAEVTLVADGRQALEALDSASFDLVLMDMQMPVMDGLEATRR
                     LRAGGGRNAAVPVLALTANVMESHRKACAEAGMNGHIAKPIDARLLLTAVLSAFDAPA
                     AATAPAMEVQAS"
     gene            2768..>4176
                     /locus_tag="PLO65_11410"
     CDS             2768..>4176
                     /locus_tag="PLO65_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD28898.1"
                     /translation="MAAPILALKDVRLADGATMLFDGVDLALEPGVRASLVGRNGAGK
                     STLLRMLAGQVQPDAGDRFASPGARLVFVPQEPVIAGATLLDFATAGGAAPHEAEAEL
                     MAFGLDPAKGARGLSGGEIRRAALARGFAEQPDVLLLDEPTNHLDIFAIQTLEERLAA
                     TRCAALIISHDRAFLERVTRRCFWPESRRVRRLDKGFAAFDDWSEQILQAEADEFRRL
                     NKALEREQHWLERGVTGRRARNEGRRRRLMAMRQQKATVLKEARGQLSMSLAAGDASG
                     KRVAEARGLSKAFGERIIVKGFSTRIQRGDRVAIVGPNGAGKTTLVKLLLGELPPDAG
                     EVKLGTGLQIAYIDQARGELKEGQTLWDVLTDGGGDQIMVRGAPRHVAGYAKEFLFQD
                     KQLRQPVASLSGGERNRLLLARALARPANLLVLDEPTNDLDMETLDLLEEMLADFDGT
                     LVLVSHDRDFIDRLASSTIA"
BASE COUNT          652 a         1455 c         1452 g          617 t
ORIGIN      
        1 gaccggctgg ccgccctctt ccacgaagac ccgtcccgcg gacggatcgc gcggcgccgg
       61 cgcggcgcct ttggggcctt gggggccttc tctggcttgg gtttcttgcc tggccgcggc
      121 gcttcgggtt cgggttcggg ctccggccgc tgttcgggcg gcggatcgaa actgtacggc
      181 ggaagggacc agtcgtcgct caaagctgtg ccgtcgcgct tgggttactg cgttcgaggg
      241 tttaggacgc aagcatcatg ccgcgcaaac ccttctggga gatcaagtct ctcgccgaga
      301 tgagtcccgc cgagtgggag agcctatgcg acggctgcgg cctttgctgc ctggttcgct
      361 tcgaggatga agaaaccggc gaggtgatcc cgacacgggt gcactgccgg ctgttcgatt
      421 cccgcagctg cacctgcacc gactacgaaa accgcaagcg gcaggtgccc gactgcatca
      481 agctgacgcc cggcaatatc gagcaactgg cctggatgcc ggccagctgc gcctatcgcc
      541 ggctcgacga gggcaagccc ctgccggcct ggcaccccct gatcaccggc gatcctgaaa
      601 gcgtgcaccg cgcgggcgtc tcgatccgcg accagacgct caacgaggac ctgctcgacg
      661 agcccgagga cgcgctcgac tacgcggcct gggatctgaa cctcgacaag agcgacgagc
      721 cctacgaggc ctgaacctcc atcgccgggg cggtcgccgc ggccggggcg tcgaacgcgc
      781 tgagcacggc ggtcagcaac agccgggcgt cgatcggctt ggcgatgtgg ccgttcatgc
      841 cggcctcggc gcaggccttg cggtggctct ccatgacatt ggcggtcagg gccagcacgg
      901 gcacggcggc attgcgaccg ccgcccgcgc gcagtcgccg cgtggcttcc agcccgtcca
      961 tcaccggcat ctgcatgtcc atcagcacca ggtcgaacga cgcgctgtcc aacgcctcaa
     1021 gggcctggcg gccatcggcg acaagggtca cttcggcgcc cagcggccgg aggaacagtt
     1081 cgatgacccg ctggttcgac ggattgtcct cggccaccag gatgcgcgcc gcggggcggt
     1141 cggcgtccgg atcgacggga ccggatccgg gagcggcgac cacggcctcg ccgacccggc
     1201 acggcaggcg cagggtgaag gtcgagccct gtccctcgac cgactgggcc cggatcgcgc
     1261 cacccatcat cccggccagt cgctggctga tcgacaggcc caggccggtg ccgccgaacc
     1321 tgcgactggt cgagccatcc gcctgttcga aggcgttgaa caggcgctgc tggacctcgg
     1381 gcgacatgcc gatgccggtg tcgctgaccg tcaggatcag gtcgatcaag cccggaccgg
     1441 tctcctccgc cgccagggtg aggccgaccc gacccgcctc ggtgaacttc agcgcgttgg
     1501 agatcaggtt gaacacgatc tggccggtgc gggtgacatc gaccacgacc cactgcggcg
     1561 tgcccggcgt gatcaccttc tcgaagacga gattcttgtc ctgggcgcgc ggagcccaga
     1621 gggcggcgca gcggcggacg aaatcgtgga tgtcggcgtt ggccgggtcg atgtcgagct
     1681 tgccggcctc gatcttggcc aggtcgagca cgtcgttgag cacctgcatc aggatcgagc
     1741 caccgtcgct gagcatcgcg acatggccac gctgctcctc ggtcagatcg gttcggccca
     1801 gcaggtcggc ggcgccgaga accgcattca tcggcgtgcg caactcgtgg ctcatggcgg
     1861 cgaggaagtc ggtcttggcc tggctggcgg cctcggcctc gcgctgcagg gccacggcct
     1921 gccgccgggc ctcgccgagc gcgccgatgg cccgctgctg ccccagccag acaaccgtca
     1981 tgtagccgac cagcaggacc gcgcaggccg aggcggtcag gccctcggcg ccgaaggcga
     2041 atggcgggat gaggaccaga taggcgatca acggcgcggc gctggcccag aacagccttc
     2101 gggcgccgag cgccgagatg atcagctgca ccagcatgcc gctcaggtac atggtgccgg
     2161 cggccacgcc cagatcgccg gcatgggtcc acaggtgcca ggccggcagg ccggcgaccg
     2221 cataggacag cagggacagg ccatagagcc gcagcggccg cgaccctgtt ggcgagaccc
     2281 ggatcaggag cagctgcagg acggcgtagg cggcccacca ggccggcgcc ggccgccagt
     2341 ccgtcatggt ccatagaagg accccgatga ccgcggtgat ggccagccgg acgacggtcg
     2401 accgggtgcg catcgacagc gccgctccca gatcgacata gtcttcctgc ctggtcatca
     2461 gattccccca cccgttcctg ctgtggcagt cgtccgttaa ggatgagttt ttggcttcca
     2521 gtatttgcag aatatcgata cgcgaattta cttactcatt acgggttatg cgccagcgtt
     2581 actcaattcg ggtcgtccgc cccgccgaaa atcggacaag gttcggcaac ggggctggcc
     2641 gccgcgtggc ggcagcccgg ccatgtcgac cggcgcaggg cttccccggc cctccccccc
     2701 ccttgccccc gggggtcgat tggggggata gaccccctcc gccttcatcc ctcgccgaaa
     2761 ccgccccatg gccgccccga ttctcgccct caaagacgtg cgtctcgccg acggcgcgac
     2821 catgctgttc gatggcgtgg acctggcgct cgaaccgggg gtgcgcgcct cgctggtcgg
     2881 ccgcaacggg gcgggcaagt cgacgctgtt gcgcatgctg gccggccagg tccagccgga
     2941 cgcgggcgat cgtttcgcca gcccgggcgc ccgcctggtg ttcgtgccgc aggagccggt
     3001 gatcgccggc gcgaccctgc tggacttcgc caccgccggc ggcgcggcgc cgcacgaggc
     3061 cgaggcggaa ctgatggcct tcggtctcga tccggccaag ggcgcgcggg ggctgtcagg
     3121 cggcgagatc cgccgcgcgg ccctggcccg ggggttcgcc gaacagcccg acgtcctgtt
     3181 gctcgacgag ccgaccaacc acctggacat cttcgccatc cagacgctcg aggaacggct
     3241 ggccgcgacg cgctgcgcgg ccctgatcat cagccacgac cgggccttcc tcgagcgggt
     3301 cacccgccgc tgcttctggc ccgagagccg cagggttcgc cggctggaca agggtttcgc
     3361 ggccttcgac gactggtcag agcagatcct ccaggccgag gccgacgaat tccgccgcct
     3421 gaacaaggcg ctggagcgtg agcagcactg gctggaacgc ggcgtcaccg gccggcgcgc
     3481 gcgcaacgag ggccgccgtc gccggctgat ggccatgcgc cagcagaagg ccacggtgct
     3541 caaggaggcg cgcggccagc tcagcatgag cctggccgcc ggcgacgcct ccggcaagcg
     3601 ggtcgccgag gcgcggggac tttcgaaggc ctttggcgag cggatcatcg tcaagggctt
     3661 ttcgacccgc atccagcgcg gcgaccgggt ggccatcgtc ggccccaacg gggcgggcaa
     3721 gaccaccctg gtcaagctgc tgctgggcga gctacccccg gacgccggcg aggtaaagct
     3781 ggggacaggc ctgcagatcg cctacatcga ccaggcgcgg ggtgagttga aggagggcca
     3841 gaccctctgg gacgtcctga cggacggcgg cggcgaccag ataatggtcc gcggcgcccc
     3901 gcgccacgtg gccggctacg ccaaggaatt cctgtttcag gacaagcagt tgcgccagcc
     3961 ggtcgcgagc ctgtcgggcg gcgagcgcaa ccgcctgctg ctggcccggg cgctggcccg
     4021 gccggccaac ctgctggtgc tcgacgagcc gaccaacgat ctcgacatgg agacgctgga
     4081 cctgctcgag gagatgctgg ccgacttcga cggcacgctt gtcctggtca gccacgaccg
     4141 cgacttcatc gaccggctgg ccagctcgac catcgc
//
LOCUS       DAORNX010000264         4166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_15957_length_4166_cov_4.993189, whole genome shotgun sequence.
ACCESSION   DAORNX010000264 DAORNX010000000
VERSION     DAORNX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15957_length_4166_cov_4.993189"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLO65_11415"
     CDS             <1..229
                     /locus_tag="PLO65_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRD28899.1"
                     /translation="GVTAGIDFALTLAAEIAGDDFAQGLQLALEYAPAPPFNAGRPET
                     APPHVLERITAFNKAAMPMRVGAAEEAAARL"
     gene            complement(431..1192)
                     /gene="ygiD"
                     /locus_tag="PLO65_11420"
     CDS             complement(431..1192)
                     /gene="ygiD"
                     /locus_tag="PLO65_11420"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRD28900.1"
                     /translation="MPVLFLGHGSPMNAIEDNPWRRGWAELAARLPRPSAVLCVSAHW
                     ETHGAAAVSAAEWPATIHDFGGFPQALFDVQYRAPGDPALATRVAALLAPDPVVQHPT
                     RGLDHGAWSVLAPMYPDADIPIVQLSLDRARSNAWHYHAGRRLAPLRDEGVLIVGSGD
                     IVHNLRDVDFRSGATPPWAPRFNDTAKALIEAGDHGPLIDWPALGPDAAASINSAEHY
                     LPLLYTLGAAGAGEPVSFFNDDVFAGISMTSVRWG"
     gene            1297..2424
                     /locus_tag="PLO65_11425"
     CDS             1297..2424
                     /locus_tag="PLO65_11425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD28901.1"
                     /translation="MTRDLSHSGASRPGQGRGGALDALRFVAALLIVLYHYGADAPSA
                     LASLHPVFARGHLATDFFLMLSGFVLGRAYGAQILGGRIGLPAFLRKRITRVWPGQLI
                     VLAAMAAVVGLALLVGLQPQNPEHYRPTAMLMQLFLVQAWGVAGGDGWNQQSWSLSAL
                     VVCYALFPLLWRAVSRVSSPVALFAFGLATVMAGDLACRALFNHSIYDLGYSLGAVRA
                     VPMFLLGVCIARIVEQGAPSVPAARALAVLALAGLVLLQVAGRFDLPSIVAIALIVLS
                     FGRLPVARPSALIEQGAKLSFALFITHALTGLVWFGALSVLRDRMALAPWMDWALWAG
                     SVPAALVVAGLFHYLVDEPLQAWLNRRPAPAPARAEAPSAA"
     gene            complement(2432..3187)
                     /locus_tag="PLO65_11430"
     CDS             complement(2432..3187)
                     /locus_tag="PLO65_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28902.1"
                     /translation="MHIDLTGKTVLVTGASRGIGAAIATAMAASGARVAVHYSVSETG
                     AAAVAAECGPDARTFGADLGDPADCVALWDAVTAAYGRVDVLVNNAGVAAPVDDPSID
                     HWLKVWNRTHDVNLRACALLCHLACEHFQAEGGGRIINISSRAAFRGDQPDYIAYAAS
                     KGGMVALTRSIARGFGKAGIVAFNVAPGFTRTAMAQDFIDQYGEDYATSDIALSRLTE
                     PKDVAPTVVFLASGLADHATGATIDINAASYVH"
     gene            complement(3243..4088)
                     /gene="nadC"
                     /locus_tag="PLO65_11435"
     CDS             complement(3243..4088)
                     /gene="nadC"
                     /locus_tag="PLO65_11435"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRD28903.1"
                     /translation="MIAPLPDLLIDPVVRAALAEDLGRAGDITALACVDADARLEATF
                     AARKAGTIAGLACARLAILALDPEARFRARVADGDPVKAGTVLAKVSANARALLSAER
                     TALNLLGRLSGVATLTRTYVDAVAGTKARITDTRKTTPGLRALEKYAVRCGGALNHRF
                     GLDDAILIKDNHVAACGGVRQAIDRARAFAGHLVRVECEVDSLDQLAEALDAGPDVVM
                     LDNFSLKNLRIAVGMTAGRVVLEASGGVTLDTVRDIAETGVDVISVGALTHSASVLDI
                     GLDAV"
BASE COUNT          533 a         1487 c         1517 g          629 t
ORIGIN      
        1 cggggtcacg gccggcatcg acttcgcgct gaccctggcc gccgagatcg ccggcgacga
       61 cttcgcccag ggcctgcagc tggcgctcga atacgccccg gccccgccgt tcaacgccgg
      121 acggcccgag accgcgccgc cgcacgtgct cgagcggatc acggcgttca acaaggcggc
      181 gatgccgatg cgggtcggcg cggcggaaga agcggcggcg cggctttagg gcccggaaat
      241 cctccccgca tcgcggggag ggggaccgcg cggcgcgcgg tggaggggcg gaacccggcg
      301 gcgaccgaca atcctgaata cgcctgtctg cccaggccgg tcgtcagggc cttaccgcga
      361 ggtccggcga cgcccctcag tcaggctccg cctgacagct ccccgcgagc ggggagaacc
      421 gtcctggacc tcagccccag cgcacactgg tcatcgatat ccccgcgaac acatcgtcat
      481 tgaagaagct aaccggctct cccgcgccgg ccgcgccgag cgtgtagagc aggggcaggt
      541 agtgctcggc gctgttgatc gaggcggccg cgtcggggcc cagcgccggc cagtcgatca
      601 gcgggccatg gtcgccggcc tcgatcagcg ccttggccgt gtcgttgaaa cgcggcgccc
      661 agggtggcgt cgcgccgctg cggaagtcga cgtcgcgcag attgtgcacg atgtcgccgc
      721 tgccgacgat cagcacgccc tcgtcgcgta gcggcgccag ccgccggccg gcgtggtagt
      781 gccaggcgtt ggacctggcc cggtcgaggc tgagctggac gatcggtatg tcggcgtccg
      841 gatacatcgg cgccagcacg ctccaggcgc cgtggtcgag gccgcgggtc gggtgctgca
      901 cgaccggatc gggcgccagc agcgcggcga cccgggttgc cagggccgga tcgcccggtg
      961 cccggtactg aacgtcgaac agcgcctggg ggaagccgcc gaagtcgtgg atcgtcgccg
     1021 gccattccgc cgccgagacc gccgccgcac cgtgggtttc ccagtgcgcc gacacgcaca
     1081 ggacagcgct cggccggggc aggcgcgccg ccagctccgc ccagccccga cgccagggat
     1141 tgtcctcgat cgcattcatc ggcgacccgt ggccgaggaa caggaccggc atgcgttcgg
     1201 ccgccacgat tcacctttta tgcaaccaac tccgatacag ataatccccg gccccgttgt
     1261 tgcaagcggc ggcgcgaaga ggccggagac ccgcgcgtga cccgagatct gtcccattcc
     1321 ggagccagcc gacccggcca gggtcgcggc ggggcgctcg acgccctgcg gttcgtcgcc
     1381 gccctgctga tcgtgctgta ccactatggc gcggacgccc cgtcggcgct ggcctcgctg
     1441 catccggtgt tcgcccgcgg ccacctggcg accgacttct tcctgatgct gtcgggcttc
     1501 gtgctcggcc gggcgtatgg agcgcagatt ctcggtggcc ggatcggcct gccggcgttc
     1561 ctccgcaagc ggatcacccg cgtctggcca ggtcagctga tcgtgctggc ggcgatggcg
     1621 gcggtggtcg gcctggccct gctggtcggc ctgcagccgc agaatccgga acactaccgc
     1681 cccacggcga tgctgatgca gctgttcctg gtccaggcct ggggcgtggc ggggggcgac
     1741 ggctggaacc agcagagctg gtcgctgtcg gcgctggtcg tctgctacgc cctgttcccg
     1801 ctgctgtggc gcgcggtcag ccgggtgtcc tcgccggtcg ccctgttcgc cttcggcctg
     1861 gcgacggtga tggccggcga cctggcctgc cgggcgctgt tcaaccattc gatctacgac
     1921 ctcggctaca gcctcggcgc ggtccgggcc gtgccgatgt tcctgctcgg cgtctgtatc
     1981 gcgcgcatcg tcgagcaggg cgccccgtcg gtcccggcgg cgcgggcgct ggccgtgctg
     2041 gcgctggcgg gcctggtcct gctgcaggtc gccggccgct tcgacctgcc gagcatcgtc
     2101 gccatcgccc tgatcgtgct gtcgttcggc cgcctgccgg tggcccgccc ctcggcgctg
     2161 atcgagcagg gcgccaagct gtcgttcgcc ctgttcatca cccacgccct gaccgggctg
     2221 gtctggttcg gcgcgctgtc ggtgctgcgc gacaggatgg ccctggcccc gtggatggac
     2281 tgggcgctgt gggccgggtc ggtccccgcc gccctggtag tggccgggct gttccactac
     2341 ctcgtcgacg agccgctgca ggcctggctc aaccgccggc ccgcgccagc ccccgcccgg
     2401 gcggaggccc cctcggccgc ctgacgccgg cctaatggac gtaggacgcc gcgttgatgt
     2461 cgatggtcgc gccggtggcg tggtcggcca ggccgctggc gaggaacacc acggtcgggg
     2521 cgacgtcctt cggctcggtc agccgggaca gggcgatgtc gctggtcgcg tagtcctcac
     2581 cgtactggtc gatgaagtcc tgggccatgg ccgtgcgggt gaagccgggc gcgacattga
     2641 aggcgacgat cccggccttg ccgaaccccc gcgcgatcga gcgggtcagg gcgaccatgc
     2701 cgcccttgga ggcggcgtag gcgatgtagt ccggctggtc gccgcggaac gccgcccgcg
     2761 agctgatgtt gatgatccgc ccgccgccct cggcctggaa atgctcgcag gccaggtggc
     2821 agagcagggc gcaggcgcgc aggttgacgt cgtgggtgcg gttccagacc ttcagccagt
     2881 ggtcgatcga cgggtcgtcg accggcgcgg ccacgccggc gttgttgacc agcacatcga
     2941 cccggccata ggcggcggtc accgcgtccc acagggccac gcagtcggcg gggtcaccga
     3001 gatccgcgcc gaaggttcgc gcgtcgggtc cgcactcggc cgccaccgcc gcggccccgg
     3061 tctccgagac cgagtaatgc acggcgaccc tggcgccgga cgcggccatc gccgtggcga
     3121 tcgccgcccc gatgccccgg ctggccccgg ttacgagcac ggtcttgccg gtcaggtcga
     3181 tgtgcatggg tgggcccctt caattcgcgc tgatcctccc cgccacggtt ggggaggaaa
     3241 tctcaaacgg cgtccagccc tatgtccagc acgctcgccg aatgggtcag ggccccgacg
     3301 ctgatcacgt ccacgccggt ctcggcgatg tcgcggacgg tgtcgagggt cacgccgccg
     3361 gacgcttcca gcacgacccg gccggcggtc atgcccacgg cgatgcgcag gttcttcagg
     3421 ctgaaattgt cgagcatgac tacgtccggc ccggcgtcga gcgcctcggc cagctggtcg
     3481 aggctgtcga cctcgcactc gaccctgacc aggtggccgg cgaaggcgcg cgcgcggtcg
     3541 atagcctgac gcaccccgcc gcaggcggcc acatggttgt ccttgatcag gatggcgtca
     3601 tccaggccga agcggtggtt cagcgccccg ccgcaacgga cggcgtactt ctccagcgcc
     3661 cgcaggcccg gcgtggtctt gcgggtgtcg gtgatgcggg ccttcgtgcc ggccacggcg
     3721 tcgacatagg tccgggtcag ggtggcgacg ccgctcagcc ggcccagcag gttcagcgcc
     3781 gtgcgttccg ctgacagcag ggcgcgggcg ttggcggaga ccttggccag caccgtcccg
     3841 gccttcaccg gatcgccatc cgccaccctg gccctgaacc tcgcctccgg atcgagagcc
     3901 aggatcgcca gccgcgcgca ggcaagcccc gcgatggtcc cggccttgcg ggcggcgaag
     3961 gtcgcctcca gacgggcgtc ggcgtcgacg caggccagcg cggtgatgtc gccggcccgt
     4021 cccaggtcct cggccagggc ggcgcggacg acggggtcga tcagcaggtc gggcagcggg
     4081 gcgatcattc ggcggcgacc ttgagggcct gctggcccgg gtgggtgacg aaggtgcggg
     4141 tcggcgggtc cttggccagc gggaag
//
LOCUS       DAORNX010000265         4126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16164_length_4126_cov_9.764923, whole genome shotgun sequence.
ACCESSION   DAORNX010000265 DAORNX010000000
VERSION     DAORNX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16164_length_4126_cov_9.764923"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLO65_11440"
     CDS             complement(<1..671)
                     /locus_tag="PLO65_11440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28904.1"
                     /translation="MDFALDPKLVAYLAELDEFIETTIMPLQAQDDNDRFFDHRREHA
                     RTDWDNGGLPRPEWEELLAKARKLADDAGHYRFAWPREMGGKDGSQLWMAVIREHLAA
                     KGLGLFNDLQNEHSIVGNNPFVLMFKEFATPEQVARDGDKLLNGTMRAAFGLTEPNHG
                     SDATFMETTGVREEKDGKPGWRIKGEKMWTTGMHVVTHVMVFARTSGKDGDALGITCF
                     IVPRE"
     gene            809..1225
                     /locus_tag="PLO65_11445"
     CDS             809..1225
                     /locus_tag="PLO65_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28905.1"
                     /translation="MILIIAAAILGAAVGLFIQPRLFALAVALSVSGAMHLVLSYLGG
                     LAAKAPDRQVLAERLALITSADVHGIWPVMAAAGAGSILAAVLWSMTQKQSTDLFWFL
                     PDSGDRRGGIRSMALVEDRDQVHEQARRKLDSMLER"
     gene            1426..1734
                     /locus_tag="PLO65_11450"
     CDS             1426..1734
                     /locus_tag="PLO65_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28906.1"
                     /translation="MKLRMLTAAAVLSLSFAGAAWADGKVTASLKTPVAAKTKIVAGG
                     AVFTCEGASCVALTAPSRSATTAGCKALAKEVGALTAYGSDRKPLQGADLEACNAAAN
                     "
     gene            1861..2112
                     /locus_tag="PLO65_11455"
     CDS             1861..2112
                     /locus_tag="PLO65_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28907.1"
                     /translation="MTDTPSLAAALERALIAAAADGRDVVPVSLTLDYGVAGDPSALA
                     ICARVDRATRSLVFVHGEARLPDGRRAAAASGVFRVRGG"
     gene            2294..2848
                     /locus_tag="PLO65_11460"
     CDS             2294..2848
                     /locus_tag="PLO65_11460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRD28908.1"
                     /translation="MADDTKATEVGQRYARALFELAEEAGATAAVEADLVSLRKAWGE
                     SADLRRLLTSPTFTSDEKGLGLAAVAEKARLNPVTRKFIGLLSANRRTGALVEIIDAF
                     RALSAARRGVIAADVVTALPLTAAQSKGLAAALRQALGKDPEISTRVDPAILGGIKVR
                     VGSRLFDASLKSKLDSLKFALKRA"
     gene            2854..>4126
                     /gene="atpA"
                     /locus_tag="PLO65_11465"
     CDS             2854..>4126
                     /gene="atpA"
                     /locus_tag="PLO65_11465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRD28909.1"
                     /translation="MDIRAAEISAILKSQIASFGEEADVSDVGSVLSVGDGIARVYGL
                     DKVQAGEMVEFPKAGVKGMALNLERDNVGVVIFGQDQQIKEGDEVRRLGEIVDVPVGR
                     GLLGRVVNPLGEPIDGKGPITNVAERRRVDVKAPGIIPRKSVHEPVQTGLKAIDTLIP
                     IGRGQRELIIGDRQTGKTAVAIDTILNQKAVNASGNEGEKLYCIYVAIGQKRSTVAQI
                     VKTLEEHGALDYTIVVAATASEPAPLQFLAPFSGCAMGEWFRDNGMHAVIIYDDLSKQ
                     AVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNEDNGSGSLTALPVIETQAN
                     DVSAYIPTNVISITDGQIFLETDLFFQGIRPAVNVGISVSRVGSSAQIKAMKTVAGPI
                     KGELAQYREMAAFAKFGSDLDAATQRQLARGE"
BASE COUNT          642 a         1443 c         1347 g          694 t
ORIGIN      
        1 tgctcgcggg ggacgatgaa acaggtgatg cccagggcgt cgccgtcctt gccgctggtg
       61 cgggcgaaga ccatcacgtg ggtgaccacg tgcatgccgg tggtccacat cttctcgccc
      121 ttgatgcgcc agccgggctt gccgtccttc tcctcgcgga cccccgtggt ttccatgaag
      181 gtggcgtcgc tgccgtggtt gggctcggtc aggccgaagg ccgcccgcat cgtgccgttg
      241 agcagcttgt cgccgtcgcg ggcgacctgc tcgggcgtgg cgaactcctt gaacatcagc
      301 acgaagggat tgttgccgac gatcgagtgc tcgttctgca ggtcgttgaa caggcccaga
      361 cccttggccg caaggtgctc gcggatcacc gccatccata gctggctgcc gtccttgccg
      421 cccatctccc ttggccaggc gaaccggtag tggccggcgt cgtcggccag cttgcgggcc
      481 ttggccagca gttcctccca ctcggggcgc ggcaggccgc cattgtccca gtcggtccgg
      541 gcgtgctcgc gccgatggtc gaagaaccgg tcgttatcgt cctgcgcctg cagcggcatg
      601 atggtcgtct cgatgaactc gtcgagctcg gcgagatagg cgaccagctt ggggtcaagg
      661 gcgaagtcca tggcgcgctc ccggaagtct cttgtggctt ttcgccagta tgcgccgcct
      721 tacgtgcgcg gcaaccgtga cccgacggaa gctcgcggac ttcgggccgt cagcgtttcg
      781 ttcacctcgg gggaggatgc tggcgcccat gatcctgatc atcgccgcag cgattctggg
      841 tgcggccgtc gggcttttca ttcagccccg gctgttcgcc ctcgccgtcg ccctgtccgt
      901 ctcgggcgcc atgcacctgg tgctgagcta tctgggcggg ctggcggcca aggcgcctga
      961 tcgccaggtg ctggccgagc ggctggcgct catcaccagc gccgatgttc acggcatctg
     1021 gccggtgatg gccgcggcag gcgccggctc gatcctggcg gcggtgctct ggtcgatgac
     1081 ccagaagcag tccaccgacc ttttctggtt cctgccggac agcggcgacc ggcggggcgg
     1141 aattcgctcc atggccctgg tcgaggatcg ggaccaggtg cacgaacagg cccgtcgcaa
     1201 gctggattcc atgctcgagc gttgagagcg attctcagaa aaatctaagc actgtgaaga
     1261 ttccgcttgc gccactttta tgatcgttga tatctaagca atgcttagtt cgtttcgcgt
     1321 cgtcggtggg gataccggcg gcagttcagc ggggcgggcg gggccagtac cgatccgagc
     1381 ttgaaacaac gatcaccacc ccgaattacg ccggagggcg aaaccatgaa actccgtatg
     1441 ctgacggctg ccgccgtcct ctccctgtcg tttgccggcg cggcctgggc cgacggcaag
     1501 gtcaccgctt cgctgaagac gcctgtcgcg gccaagacca agatcgtggc cggcggcgcc
     1561 gtgttcacct gcgagggcgc cagctgtgtc gcgctcaccg cgccgtcgcg ctccgccacc
     1621 acggcgggct gcaaggccct cgccaaggaa gtgggcgccc tgaccgccta tggcagcgac
     1681 cgcaagccgc tccagggcgc cgacctggaa gcctgcaacg ccgcggccaa ctagtctttg
     1741 ctgccccggg cggcccgccg tccggtttgt ttcctcgagc aactcgccag tggcgcaggg
     1801 cggcggtttc gaccgccgcc ctttttcttt gtcacgggcg acggtaaggc agccgccgac
     1861 atgaccgaca cgccttccct cgccgcggcg cttgaacgcg ccctgatcgc ggccgccgcc
     1921 gatggccgcg acgttgttcc ggtatccctg acgctcgact acggcgtcgc gggcgatccc
     1981 tccgccctcg ccatatgcgc gcgcgtcgac cgggccaccc gcagcctggt gttcgtccac
     2041 ggtgaggcgc gcctgcccga cgggcgtcgc gccgcggccg cctccggcgt atttcgggta
     2101 aggggcggct agcttagggc cgcccgcgcc gttgcgatgc aacagaacgc atgctatcag
     2161 gcgcgcggct tcgggccgga ggacattcac ggcccgccgg ctcatgtgct tttccaagca
     2221 ctttcaagac tttagcgccg cgccaagggg catacccagg acgcggtgcg cgacgcaccg
     2281 gacggttgaa caagtggcgg acgacaccaa ggcgacggaa gtaggccagc gctacgcgcg
     2341 agccctgttt gaactggccg aggaggccgg tgcgacggcg gcggtggagg cggacctggt
     2401 cagtctcagg aaggcctggg gcgaaagcgc cgacctgcgc cgtctactga cctcgccgac
     2461 cttcacatcc gacgaaaagg gcctcggcct ggccgccgtg gccgagaagg cccggctgaa
     2521 cccggtcacc cgcaagttca tcggcctgct gtcggccaac cgtcgcaccg gcgccctggt
     2581 cgagatcatc gacgccttcc gcgccctttc ggccgcccgg cgtggcgtca tcgccgccga
     2641 cgtggtcacg gcgctgccgc tgaccgccgc ccagagcaag gggctcgccg ccgccctgcg
     2701 tcaggccctg ggcaaggacc ctgaaatctc gacccgtgtc gatcccgcca ttctcggcgg
     2761 catcaaggtg cgcgtcggtt cccgcctgtt cgatgcttcg ctgaagtcga agctcgactc
     2821 cctgaaattc gccctcaaga gagcgtaaga cctatggata tccgcgccgc cgaaatttcg
     2881 gccatcctca agtcgcagat cgccagcttc ggcgaggaag ccgacgtctc cgacgtcggg
     2941 tccgtgctga gcgtcggcga cgggatcgcc cgcgtctacg gactggacaa ggtccaggcc
     3001 ggcgaaatgg tcgaattccc caaggccggg gtcaagggca tggccctgaa cctcgagcgc
     3061 gacaacgtcg gggtcgtgat cttcggccag gaccagcaga tcaaggaagg cgacgaagtc
     3121 cgccgtctgg gcgagatcgt cgacgttccg gtcggacggg gcctgctggg ccgcgtcgtc
     3181 aacccgctgg gcgagccgat cgacggcaag ggcccgatca ccaacgtggc cgagcgccgc
     3241 cgggtcgacg tcaaggctcc gggcatcatc ccgcgcaagt cggtgcatga gccggtgcag
     3301 acgggcctca aggccatcga caccctgatc ccgatcggcc gcggccagcg cgagctgatc
     3361 atcggcgacc gtcagaccgg caagaccgcc gtggcgatcg acaccatcct caaccagaag
     3421 gccgtcaacg cctcgggcaa cgagggcgag aagctctact gcatctacgt cgccatcggc
     3481 cagaagcgct cgaccgtcgc ccagatcgtc aagaccctcg aggagcatgg cgcgctcgac
     3541 tacaccatcg tcgtcgccgc gaccgcctcc gagccggccc cgctgcagtt cctggcgccc
     3601 ttctcgggct gcgccatggg cgagtggttc cgcgacaacg gcatgcacgc cgtcatcatc
     3661 tatgacgacc tttccaagca ggccgtggcc tatcgccaga tgtccctgct gctgcgccgt
     3721 ccgccgggcc gcgaagccta tccgggcgac gtcttctacc tgcacagccg cctgctcgag
     3781 cgggccgcca agctgaacga ggacaacggc tccggctcgc tgaccgccct gccggtcatc
     3841 gagacccagg ccaacgacgt gtcggcctac attccgacca acgtgatctc gatcaccgac
     3901 ggccagatct tcctcgagac cgacctgttc ttccagggca tccgcccggc cgtgaacgtc
     3961 ggcatctcgg tgtcgcgcgt gggctcctcg gcccagatca aggccatgaa gaccgtcgcc
     4021 ggcccgatca agggcgagct cgcccagtac cgcgagatgg ccgccttcgc caagttcggc
     4081 tccgacctcg acgccgccac ccagcgccag ctggcccgcg gcgagc
//
LOCUS       DAORNX010000266         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16178_length_4124_cov_6.289998, whole genome shotgun sequence.
ACCESSION   DAORNX010000266 DAORNX010000000
VERSION     DAORNX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16178_length_4124_cov_6.289998"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..77
                     /locus_tag="PLO65_11470"
     CDS             <1..77
                     /locus_tag="PLO65_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28910.1"
                     /translation="QGLRFAIREGGRTVGAGVVAKIVE"
     gene            331..1785
                     /gene="zwf"
                     /locus_tag="PLO65_11475"
     CDS             331..1785
                     /gene="zwf"
                     /locus_tag="PLO65_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRD28911.1"
                     /translation="MPQAAPVLVLLGGAGDLAMRMLLPSLYHLELDHLLPAGLRIIAV
                     GRAPGDADSYRAEVKADMERRDLFDTDGWDRLSARLDYCAADAGQAEGARTLAGRIGQ
                     HGELVFFYALSPSLFAPVTRALQAAGLTGERTRLVLEKPIGRDLESSRQINAAIAAAV
                     PESRVFRIDHYLGKETVQNLIALRFANTLFEPLWTNVSIDHVQITVAETHGVGDRWPY
                     YDDYGAIRDMLQNHMLQLLCLVAMEPPSDLEPDAVRNEKVKVLRSLRPFTRASAASDS
                     VRGQYGDGVVDGRAVESYESEVGRASSTETFVALTAWIDNWRWAGVPFFLRTGKRMAE
                     RCTEIVIQFKPVPHSIFGGAAVGDLQANRLVIALQPDEDIALTIMNKRPGLTQEVMRL
                     QPLPLSLSLAKALGEEGGRRRIAYERLLLDALRGDPTLFVRNDEVEAAWRFVDGVADA
                     WVEAGMAPRPYPAGSWGPGGAFALIERSGRSWNE"
     gene            1778..2467
                     /gene="pgl"
                     /locus_tag="PLO65_11480"
     CDS             1778..2467
                     /gene="pgl"
                     /locus_tag="PLO65_11480"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRD28912.1"
                     /translation="MNSRPVMEVCETPEAAALAAAEAIADWLAAGLAENDHARFVATG
                     GATPGPVYDLLSTLPLPWDQVSVTLSDERWVRPTSPESNERQLRARLLVGPAAAARFI
                     PLWSDAPTAEEAVEAAEAALEDMFPADVILLGMGEDGHIASLFPGSPALEEGLDPFGG
                     SLVVAVPAGDPAPAIERLSLTLHALRQAFLTIVLIRGEAKRRILEDRDDLPIHALFRA
                     TDMPVRVIWSP"
     gene            2472..>3797
                     /locus_tag="PLO65_11485"
     CDS             2472..>3797
                     /locus_tag="PLO65_11485"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD28913.1"
                     /translation="METPVNPVVLEVTARIVERSRPTRTAYLAHIDAARAGDPGRARL
                     SCANWAHAFAGADPKDRLRALDPTAPNLGIVSAYNDMLSAHQPLERYPALIKAAAREV
                     GATAQFAGGVPAMCDGVTQGRPGMELSLFSRDVIAMATAVALTHDAFDGGLFLGVCDK
                     IVPGLAIGALTFGHLPAIFVPAGPMTSGLPNAEKARIRALYAEGKATREELLAAEQAS
                     YHGPGTCTFYGTANSNQMLMEMMGLHLPGSAFIHPGTPLRDALVAAAAKRCAAIGPRS
                     NDWTPSGHVIDEKAVVNGLVGLMATGGSTNHVLHMVAVARAAGILLRLEDFDDLSRAT
                     PLLARVYPNGSADVNQFHAAGGIAFVVRELLRAGLVHDDVLTVAGRGLHRYAREPHLD
                     GEALAWRDGATQSLDRAILRPADDPFEREGGLRRLAGVLGQAVIKVSAVK"
     assembly_gap    3798..3846
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3847..>4124
                     /locus_tag="PLO65_11490"
     CDS             <3847..>4124
                     /locus_tag="PLO65_11490"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD28914.1"
                     /translation="DALQEAFRRGALDRDFVAVVRFQGPAANGMPELHALTPALSVLQ
                     DRGHRVALVTDGRMSGASGKVPAAIHVTPEAAVGGPLARVRDGDMIRL"
BASE COUNT          570 a         1472 c         1434 g          599 t
ORIGIN      
        1 accagggcct gcgcttcgcc atccgcgaag gcggccgcac cgtcggcgcc ggcgtggtgg
       61 ccaagatcgt cgagtaggat ctacctgcgt ccttcgacac gccgctgcgc ggctgctcag
      121 gatgacgggc gctggcgtac gtcatggtga gcagcgaccg ccggtcgcgt gtcgaaccac
      181 gcaacacccg atcaggcccc ccggcggaaa cgccgggggg ttttttcttt gcgcgccccc
      241 cgcgcccgaa acctgaaccc tccccgcctt caaacgctga cgtagcgtcg cgcccccgct
      301 aagacagggg gtgtccgcga aggagccgtc atgccgcaag ccgcccccgt tctggtcctt
      361 ctcggcggcg cgggcgatct ggcgatgcgg atgctgctgc cgtcgctgta tcaccttgag
      421 ctcgaccacc ttctgccggc cggcctgcgg atcatcgcgg tcggccgggc gcccggggac
      481 gcagacagct atcgcgccga ggtgaaggcc gatatggaaa ggcgcgacct gttcgacacc
      541 gacggctggg accggctcag cgcccgcctc gactactgcg ccgccgacgc gggccaggcc
      601 gagggggcca ggacgctggc cgggcggatc ggccagcacg gcgagcttgt gttcttctac
      661 gccctgtcgc ccagcctgtt cgcgccggtc acccgggcgc tgcaggcggc gggcctgacc
      721 ggcgagcgca cccggctggt gctggaaaag ccgatcggcc gcgatctgga aagctcgcgc
      781 cagatcaacg ccgccatcgc cgccgccgtg cccgagagtc gcgtgttccg catcgaccac
      841 tacctgggca aggagacggt gcagaacctg atcgccctgc gtttcgccaa caccctgttc
      901 gagccgctgt ggaccaacgt cagcatcgac catgtgcaga tcaccgtggc cgagacccac
      961 ggcgtgggcg accgctggcc ctattacgac gactatggcg ccatccgcga catgctgcag
     1021 aaccacatgc tgcagctgct ctgcctggtc gccatggagc cgccgtcgga cctcgagccg
     1081 gacgcggtgc gcaacgaaaa ggtcaaggtg ctgcgctccc tgcggccgtt cacccgcgcc
     1141 tcggcggcca gcgacagcgt gcgggggcag tacggagacg gtgtggtcga tggccgggcc
     1201 gtcgaaagct atgagtccga agtcggccgg gcgtcctcga ccgagacctt cgtcgccctg
     1261 acggcctgga tcgacaactg gcgctgggcc ggggtgccct tcttcctgcg caccggcaag
     1321 cgcatggccg agcgctgcac ggaaatcgtc atccagttca agccggtgcc ccattcgatc
     1381 ttcggcggcg ccgccgtggg ggatctgcag gccaatcggc tggtcatcgc gctgcaaccc
     1441 gacgaggaca tcgccctgac gatcatgaac aagcgaccgg gcctgaccca ggaggtcatg
     1501 cgcctgcagc ccctgccgct gtcgctgagc ctggccaagg ccctcggcga ggagggcggc
     1561 cggcggcgca tcgcctacga gcggctgctg ctcgacgccc tgcgcggcga cccgacgctg
     1621 ttcgttcgca atgacgaggt cgaggcggcc tggcggttcg tcgacggcgt ggcggacgcc
     1681 tgggtcgagg ccggcatggc gccgaggcct tatccggccg ggtcctgggg cccaggcggc
     1741 gccttcgccc tgatcgagcg gtcgggacgg tcctggaatg aatagccggc cggtcatgga
     1801 ggtctgcgag acgccggagg cggccgccct ggccgccgcc gaggccatcg ccgactggct
     1861 ggccgcaggc ctggccgaga acgaccacgc ccgtttcgtc gccaccggcg gcgcgacccc
     1921 cggaccggtc tacgatctgc tgtcgaccct gccgctgccg tgggaccagg tcagcgtgac
     1981 cctgtcggac gagcgctggg tccggccgac ctcgcctgag tccaacgagc gccagctgcg
     2041 cgcgcgactg ctggtcggac cggcggcggc cgcccggttc ataccgctat ggtccgacgc
     2101 gccgaccgcc gaggaggccg tcgaggcggc cgaggccgcg ctggaggata tgttcccggc
     2161 ggacgtcatc ctgctcggca tgggcgagga tggccatatc gcctcgctgt ttcccggcag
     2221 cccggcgctg gaggaggggc tcgatccgtt cggcggcagc ctggtggtcg ccgtgccggc
     2281 cggcgacccg gcgccggcca tcgaacgcct gagcctgacg ctccacgccc tgcggcaggc
     2341 ctttctgacg atcgtgttga tacggggcga ggcgaaacgc cgaatcctcg aggatcggga
     2401 cgacctgccg atccacgcgt tgttcagggc gaccgacatg cccgtgcgcg tgatctggtc
     2461 gccctgacac catggagacg cccgtgaacc ccgtcgttct ggaggtcacc gcccgcatcg
     2521 tcgagcgcag ccgtccgacc cgcaccgcct atctggcgca catcgacgcg gcccgcgccg
     2581 gcgacccggg ccgtgccagg ctcagctgcg ccaactgggc acacgccttc gccggggccg
     2641 atccgaagga ccgcctgcgg gcgctcgatc cgaccgcccc caacctcggg atcgtctcgg
     2701 cctacaacga catgctcagc gcccaccagc cgctcgagcg ctatccggcc ctgatcaagg
     2761 ccgcggcccg cgaggtcggg gccacggccc agttcgccgg tggcgtgccg gccatgtgcg
     2821 acggcgtcac ccagggccgg cccggtatgg agctctcgct gttcagccgc gacgtgatcg
     2881 ccatggccac ggccgtcgcc ctgactcatg acgccttcga cgggggcctg tttctcggcg
     2941 tctgcgacaa gatcgtgcct ggcctcgcca tcggggccct gaccttcggc catctgccgg
     3001 cgatcttcgt cccggccggg ccgatgacct ctggcctgcc caacgccgag aaggccagga
     3061 tccgcgccct ctacgccgag ggcaaggcga cccgcgagga actgctggcc gccgagcagg
     3121 ccagctacca cggcccgggc acctgcacct tctacggcac ggccaattcc aaccagatgc
     3181 tgatggagat gatgggcctg cacctgccgg gctcggcctt catccatccg ggcacgccgc
     3241 tgcgcgacgc cctggtcgcg gcggccgcga agcgttgcgc ggcgatcggg ccccgctcga
     3301 acgactggac gccatcgggc cacgtcatcg acgagaaggc ggtggtcaac ggcctggtcg
     3361 gactgatggc cacgggcggc tcgaccaatc acgtgctgca catggtcgcc gtggcccggg
     3421 cggccggtat tctgctgcgg ctcgaggatt tcgacgacct gtcccgggcg acgccgctgc
     3481 tggcgcgcgt ctatcctaac ggctcggccg atgtgaacca gttccatgcc gccggcggca
     3541 tagccttcgt ggtgcgcgag ctgttgcggg cgggcctggt ccacgacgat gtgctgaccg
     3601 tcgccggccg cggcctgcat cgctacgccc gggagccgca tctggacggc gaggcgctgg
     3661 cctggcgcga cggggccacg caaagcctcg accgggcgat cctgcgaccc gccgacgatc
     3721 cgttcgagcg cgagggtggg ctgaggcggc tggccggcgt cctgggccag gcggtcatca
     3781 aggtctcggc ggtgaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnggac gcgctgcagg aggccttccg gcgcggcgcg ctggaccggg acttcgtcgc
     3901 cgtcgtccgg ttccagggcc ccgcggccaa cggcatgccc gaacttcacg cgctgacgcc
     3961 ggccctgtct gtgctgcagg accgcggcca ccgggtggcg ctggtcaccg acggccggat
     4021 gtccggcgcg tcgggcaagg tgccggcggc catccatgtg acgccggagg cggctgtcgg
     4081 cggcccgctg gccagggttc gggacggcga catgatccgc ctgg
//
LOCUS       DAORNX010000267         4123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16182_length_4123_cov_9.091691, whole genome shotgun sequence.
ACCESSION   DAORNX010000267 DAORNX010000000
VERSION     DAORNX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16182_length_4123_cov_9.091691"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLO65_11495"
     CDS             complement(<1..349)
                     /locus_tag="PLO65_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HRD28915.1"
                     /translation="MSLLKQPTFAERQQAAAEAKKAMLAKFKPKPAVVDPNFVHREER
                     LAAEREAARAARELAKEEARKAAEARAEAQRLAEAKAAEERRLALLNDEEAQLALKRE
                     ERKARKAEAKAEAR"
     gene            471..863
                     /locus_tag="PLO65_11500"
     CDS             471..863
                     /locus_tag="PLO65_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD28916.1"
                     /translation="MTPAPIQKWHAYLETLDAAALKDLLDEDVVFESPVVHTPQAGRP
                     ITMKYLLGAAAVLNNDSFHYEHEWFSPTGAVLEFVTEIDGIRINGVDIIHWNEAGRIT
                     RFKVMVRPLKAINLLHQRMGAMLASMAG"
     gene            complement(860..1315)
                     /locus_tag="PLO65_11505"
     CDS             complement(860..1315)
                     /locus_tag="PLO65_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD28917.1"
                     /translation="MRIVDFEPRHAPAWRALNEAWIGRHFAIEPADEVVLADPVGQVV
                     GPGGHIFMAEDADGRAIGCVGLKAMEGGGYEVIKMTVAEAARGTGLGRDLMRACIDRA
                     KELGAPRLYLETNSSLGPALGLYRAMGFVDLPRHDTPYARCDVWMALEL"
     gene            1376..2536
                     /locus_tag="PLO65_11510"
     CDS             1376..2536
                     /locus_tag="PLO65_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRD28918.1"
                     /translation="MVHPVYADLPTTIFEVMSGLARETGAINLGQGFPDEPGPEAVRR
                     RAAEEALTGYNQYPPMRGLPELRRAAADHYARFQGLDLDWQTEVTVTSGATEALAAAF
                     LSLVSPGDEVLLFQPLYDAYAPMVRRAGGVPRLATLAPPHWRITRDMLEAAFTPKTRM
                     VVLNNPVNPAAVIFPGEDLALLAEFCVAHDAIAVCDEVWEQIVFDGRTFRSMMSFPGM
                     RERTVKIGSAGKMFALTGWKVGFLCAAPALTRGLAGAHQFLTFTTPPPLQAAVAWGLD
                     NCADWFATMPGQLQASRDRLAAGLTREGFTVLPAAGTYFLNVDLAASGVTEDDRAFCL
                     RAVRDHGVAAIPVSAFYEQDAPPHIIRLCFAKRDETLDAGVERLAAARAAAC"
     gene            complement(2538..3116)
                     /locus_tag="PLO65_11515"
     CDS             complement(2538..3116)
                     /locus_tag="PLO65_11515"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD28919.1"
                     /translation="MPEADIATLLAFFKAMANESRLRIIGLVAERERSVQELAELLNL
                     KEPTVSHHLAALKALDLLHVRVEGVTHWHRLRPEGLHDFNRALFDQKDLTALAKPEQT
                     WEEKVIANFLLPDGTLKTLPASRRKRWVLLKWQMSGFEEGRRYAEKEVNAILQRRHPD
                     VATIRREMIGWKMLAREDNVYWRLPEDQWEEA"
     gene            complement(3368..>4123)
                     /locus_tag="PLO65_11520"
     CDS             complement(3368..>4123)
                     /locus_tag="PLO65_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD28920.1"
                     /translation="FDLTGKVAIITGSSRGIGKAIAERMAQHGAKVTISSRKAGPCDE
                     VAAEINGTYGAGTAIAVPANISSKEDLQRLVDETRKAFGKVDVVVCNAASNPYYGPLA
                     GIADDQFRKILDNNIISNHWLINMVIPEMIERKDGAVIIVSSIGGLRGSPVIGAYNIS
                     KAADFQLARNLAHEYGKHGIRVNCIAPGLIKTDFAKALWDNPETLKRSTETVPLRRIG
                     EPDEIAGAAVFLASAAGTFMTGQAVVVDGGATI"
BASE COUNT          593 a         1402 c         1419 g          709 t
ORIGIN      
        1 cgcgggcctc ggccttggct tcggccttgc gggccttgcg ttcctcgcgc ttgagcgcga
       61 gctgggcttc ctcgtcgttg agcagggcca ggcggcgctc ctcggcggcc ttcgcctcgg
      121 cgaggcgctg ggcctcggcc ctggcctcgg ccgccttgcg ggcctcctcc ttggccagtt
      181 cgcgggccgc gcgagcagcc tcgcgctcgg cggccaaccg ctcctcgcgg tggacgaagt
      241 tggggtcgac gacggcgggc ttcggcttga acttggccag catggccttc ttggcttcgg
      301 ccgcggcctg ttggcgctcg gcgaaggtcg gttgcttcag cagactcata ggatctttcg
      361 gcgattttac acgggggctt caaagcgaaa attccgccgc gaggcaagcc cggtttgacc
      421 ccttcgtcag cccctgtggt agaaccggct caccatcccc ggaggaaacc atgacgcccg
      481 cgccgatcca gaagtggcac gcctatctcg agacgctcga cgccgcggcg ctcaaggatc
      541 tgctggacga ggacgtcgtc ttcgaatcgc ccgtggtcca cacgccccag gccggccggc
      601 cgatcaccat gaaatacctg cttggggcgg cggcggtgct caacaacgac agcttccact
      661 acgagcacga atggttctcc ccgacgggcg ccgtgctgga gttcgtcacc gagatcgacg
      721 gcatccgcat caacggcgtc gacatcatcc attggaacga ggccggcagg atcacccgct
      781 tcaaggtcat ggtgcggccg ctgaaggcga tcaacctgct gcaccagagg atgggcgcca
      841 tgctcgccag catggccggc tagagctcca gcgccatcca gacgtcgcag cgggcgtagg
      901 gcgtgtcgtg ccgcggcagg tcgacgaagc ccatggcgcg gtagaggccg agggccgggc
      961 ccaggctgct gttggtctcc aggtagagcc tgggcgcgcc cagctccttc gctcggtcga
     1021 tgcaggcgcg catcaggtcg cggcccaggc ccgtgccgcg ggcagcctcg gccacggtca
     1081 tcttgatcac ctcgtagccg ccaccctcca tggccttcag gcccacgcag ccgatggcgc
     1141 ggccgtccgc atcctcggcc atgaagatgt gaccgcccgg cccgacgacc tggccgaccg
     1201 gatcggccag cacgacctcg tcggccggct cgatcgcgaa gtgccggccg atccaggcct
     1261 cgttcagcgc ccgccaggcc ggcgcatggc ggggttcgaa atcgacgatg cgcatggcat
     1321 gagtttggga actgccgcag catcccgcaa gccggtcgcg caaggagccc cagtcttggt
     1381 tcatcccgtc tacgccgacc tgccgacgac gatcttcgag gtgatgagcg gcctcgcccg
     1441 cgagaccggg gcgatcaacc tgggccaggg ctttcccgac gaaccgggtc ccgaggcggt
     1501 gcgtcgccgc gcggccgagg aggcgctgac cggctacaac cagtatccgc cgatgcgcgg
     1561 cctgcccgag ctgcggcggg cggccgccga ccactatgcg cggttccagg gcctggacct
     1621 cgactggcag accgaggtga cggtgacttc cggcgccacc gaggccctgg ccgcggcctt
     1681 cctgagcctg gtctcgccgg gcgacgaggt gctgctgttc cagccgctct atgacgccta
     1741 cgccccgatg gtccgccgcg ccggcggcgt tccgcggctg gccaccctgg cgccgccaca
     1801 ctggcgcatc acgcgcgaca tgctcgaagc ggctttcacg ccgaagaccc gcatggtggt
     1861 gctgaacaat ccggtcaatc cggcggcggt gatcttccct ggcgaggacc tggccctgct
     1921 ggccgagttc tgcgtggccc acgatgcgat cgccgtctgc gacgaggtct gggagcagat
     1981 cgtcttcgac ggccggacct tccgctcgat gatgagcttc cccggcatgc gcgagcggac
     2041 ggtcaagatc ggttcggccg gaaagatgtt cgccctgacc ggctggaagg tcggcttcct
     2101 gtgcgccgcg ccggccctga ccagggggct ggccggggcg caccagttcc tgaccttcac
     2161 cacccccccg ccgttgcagg ccgccgtcgc ctggggcctg gacaactgcg cggactggtt
     2221 cgcgaccatg ccgggccagc ttcaggcgtc gcgggaccgg ctggcggccg gactgacacg
     2281 ggaggggttc accgtgttgc cggcggcggg gacctatttc ctcaacgtcg atctggcggc
     2341 gtcgggggtc accgaagatg accgggcctt ctgcctgagg gcagtcaggg accacggcgt
     2401 ggcggcgatc cccgtgtcgg ccttctacga acaggacgcg ccgccccaca tcatccgcct
     2461 gtgcttcgcg aagcgcgacg aaacccttga cgcgggcgtg gagcggctgg cggcggcgcg
     2521 cgccgcggcc tgctaggtca cgcctcttcc cactggtcct ccggcaggcg ccagtacacg
     2581 ttgtcctcgc gggccagcat tttccagccg atcatctcgc ggcgaatggt ggcgacgtcg
     2641 gggtggcggc gctggaggat ggcgttgacc tccttctccg catagcggcg gccttcctcg
     2701 aagcccgaca tctgccactt gagcagcacc cagcgcttgc ggcggctggc cggcagggtc
     2761 ttcagcgtgc cgtcaggcag gaggaagttg gcgatgacct tctcctccca ggtctgctcc
     2821 ggcttcgcca gggcggtcag gtccttctgg tcgaacaggg cgcggttgaa gtcgtgcagg
     2881 ccttcggggc gcagcctgtg ccagtgggtg accccctcta cccgcacatg cagcaaatcc
     2941 agcgccttga gggccgccag gtggtgggag accgtgggct ccttcaggtt cagcagctcg
     3001 gccagctcct gcacgctgcg ctcgcgctcg gcgacgaggc cgatgatgcg cagacggctc
     3061 tcattggcca tggccttgaa gaaggccagc agggtggcga tgtcggcttc gggcacgggc
     3121 gcagatccat tgatctgcgt ctaattagat gatcatcaat gaatcttcaa gccggatgta
     3181 gagtcgccct ctggccttga tggaatgatg cgctccggac gtcggacctg gtcaaggacg
     3241 acccccttcg gggtcgccgc tgcgcggccg ccgaaggcgg tccttgagca gggccgtcgc
     3301 cggagcaaga ctggccttca agacatcagg gctggtccgc ccctggagcg gtccgtccga
     3361 gcgctgccta gatcgtcgcg ccgccatcca cgacgaccgc ctggccggtc atgaacgtcc
     3421 ccgccgccga ggccaggaac accgccgcgc cggcgatctc gtccggttcg ccgatgcggc
     3481 gcagcggcac ggtctcggtc gagcgcttga gggtctcggg attgtcccac agggccttgg
     3541 cgaagtcggt cttgatcagg cccggcgcga tgcagttcac gcggatgccg tgcttgccgt
     3601 actcatgggc caggttgcgg gccagctgga agtcggccgc cttggagatg ttgtaggccc
     3661 cgatcaccgg gctgccgcgc aggccgccga tcgaggagac gatgatcacc gccccgtcct
     3721 tccgctcgat catctccggg atgaccatgt tgatgagcca gtggttggag atgatgttgt
     3781 tgtcgagaat cttgcggaac tggtcgtcgg cgatgccggc cagcggtccg tagtaggggt
     3841 tggacgcggc gttgcagacg acgacatcga ccttgccgaa ggccttgcgc gtctcgtcga
     3901 ccagccgttg caggtcttcc ttggacgaga tgttggccgg gacggcgatg gccgtgcctg
     3961 cgccgtacgt gccgttgatc tcggcggcga cctcgtcgca ggggccggcc ttgcgcgagg
     4021 agatggtcac ctttgcgcca tgctgggcca tgcgttcggc gatcgccttg ccgatgccgc
     4081 gcgagctgcc ggtgatgatc gcgaccttgc cggtcagatc gaa
//
LOCUS       DAORNX010000268         4115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16231_length_4115_cov_6.078818, whole genome shotgun sequence.
ACCESSION   DAORNX010000268 DAORNX010000000
VERSION     DAORNX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16231_length_4115_cov_6.078818"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4115)
                     /locus_tag="PLO65_11525"
     CDS             complement(<1..>4115)
                     /locus_tag="PLO65_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRD28921.1"
                     /translation="LAAGFGGGLVTASVLGGGFGGKGGGPAGSVASGPTEKPSLWSMF
                     GKPRAAGAPRRGIPKPDGFAVWKTRLDTSKADPLACIEMTRPLDPGKAYADYVLVSPD
                     LGGTPAVSVNQKNRAELCIGGLGLTERRVTLLKGLPGAGGQTLAANADVDFTFGEKPP
                     YVGFAGDGVILPREDADGVGIETVNVTRLSIEVWRVADRNLVRTSISAPDPTGEGDWP
                     GDYGDDSPNDEGRVVWKGDVAVRGGAGERVTTVFPLGAVLKDMQPGGYVIKARDASSR
                     PTAADDQDEESEGVQPAQARRWIVFTDMAMIAYDGAESLDVVVRSLKSARVLPGTRVA
                     LVAKNGETLADARTDSAGRVRFLRPLLEGDGALHPRMVMAYGAQGDLAVLDLDRSPVD
                     LSKQGMGGRNAPAADDDETGGRVADSEIDGFLYADRGIYRPGETVHLSALIRDLQSRA
                     VKDRKGVLIIRRPSGVEFKRVAFDRAPTGAVTADVVLPRSAPRGRWKASLEIEGVKAA
                     AGEMAFSVEDFAPQRLAVSTEGRETTPLAAGETRQVSVMARFLYGAAGAGLQTQGEAR
                     IMADPNPFPAFKGYEWGDQQTTFAEKFIELGSSVTDGEGRAVLSVAASEAGDTTAPLK
                     ATVTASVFEPGGRPVRESLFLKVRTRPVYLGVKVEQTDAAGGSAAPVTLDFIAVNAQG
                     ERIAAPGASYTLIAENWDYDWFQQDGRWQWRRTSRDVVVQKGALNIGAGGAARIARRL
                     GWGDYRVVVEGPGGAKSVIRFAAGWGAPAKDAEAPDFVRISAGTGVHAQGDSVTLTIK
                     APYAGEAQVAVATDRVIDFKTFSVGPNGGSVTLKTDAAWGGGAYVLVSVIQPRDPAAT
                     PKPRRALGLMYVPLDPKGRKLAVELGTPVKLNSRAPVEVPVTVRGLGLGQRARVTVAA
                     VDEGILRLTRFDAPDPVKWYFGKRALTLDYRDDYGRLLDPNLGAPANVNFGADQLGGE
                     GLTVTPIKSVALWSGVVETGLDGKARIKLPAADFNGELRVMAVAWTDEAVGSAAKAMT
                     VREAVIADLNLPRFLAPGDRPFATLELHNVEGAAGGYTAETSSSGGVVMTFRKLFQLA
                     LGQRVAERIPFLAPTRSGVGKVGFKVAGPNFSTAKDYPIQTRLGWSPITRTTVELQRP
                     GEAFTPPAQLLSGLAAGDVSLQVSYSPFRGFDPAPIAVALSRYPYGCTEQVVSVAYPL
                     LFARELAADPKKRSSSAALNDAVGRLLDRQTLDGAFGLWRVGDGEADAWLGAYATDFL
                     WEARLRGAPVPEAAMERALGAMRQISRPEGWASVSYRLEYPESWYRNEAESKKATERM
                     RSRASAYALYVLAKAGRGDLARLRWWHDVHMKDDDSPLARAQVGAGLALMG"
BASE COUNT          555 a         1522 c         1461 g          577 t
ORIGIN      
        1 cgcccatcag ggccagcccc gcgccgacct gggcccgggc caggggcgag tcgtcgtcct
       61 tcatatggac gtcgtgccac cagcgcagcc gggccaggtc gccgcggccg gccttggcca
      121 gaacatacag ggcgtaggcc gaggcgcggc tgcgcatccg ctcggtggcc ttcttcgact
      181 cggcctcgtt gcgataccag ctttccggat actcgaggcg gtaggacacg ctggcccagc
      241 cctccggccg gctgatctgc cgcatcgcgc caagcgcccg ctccatcgcc gcctcgggca
      301 cgggcgcgcc gcgcagccgg gcttcccaca ggaagtcggt ggcgtaggcg cccagccagg
      361 cgtcggcctc gccgtcgccg acccgccaca ggccgaaggc cccgtcgagg gtctggcgat
      421 cgagcagccg gccgaccgcg tcgttcaacg ccgccgagga cgagcgcttc ttcggatcgg
      481 ccgccagttc gcgggcgaac agcagcggat aggcgaccga gaccacctgt tcggtgcagc
      541 catagggata gcgcgacagg gccacggcga tcggcgccgg gtcgaagccg cggaacggcg
      601 agtagctgac ctgcaggctg acatcgcccg ccgccagacc cgacagcagc tgcgccggcg
      661 gcgtgaaggc ctcgcccggc cgctgcagct cgaccgtcgt gcgggtgatc gggctccagc
      721 ccaggcgggt ctggatcgga tagtccttgg ccgtcgagaa gttcggcccg gcgaccttga
      781 agccgacctt gccgacaccc gagcgggtcg gcgccaggaa cgggatccgt tcggcgaccc
      841 gctggccgag ggccagctgg aacagcttcc tgaacgtcat caccacgccg cccgaggaac
      901 tggtctcggc cgtgtagccg ccggcggcgc cctcgacgtt gtgcagctcg agcgtcgcga
      961 acggccgatc gcccggcgcc aggaagcgcg gcaggttgag gtcggcgatc accgcctcgc
     1021 gcaccgtcat ggccttggcg gccgagccga cggcctcgtc cgtccaggcc acggccatga
     1081 cccgcagctc gccgttgaag tcggcggccg gcagcttgat gcgcgccttg ccgtcgagcc
     1141 cggtctcgac cacgcccgac cacagggcca ccgacttgat cggggtcacg gtcaggccct
     1201 cgccgccgag ctggtcggcg ccgaagttga cgttggccgg cgccccgagg ttcggatcga
     1261 gcaggcggcc gtagtcgtca cggtagtcga gcgtcagggc gcgcttgccg aagtaccatt
     1321 tcaccggatc gggcgcgtcg aagcgggtca ggcgcaggat gccctcgtcg accgccgcga
     1381 cggtcacgcg ggcgcgctgg ccgaggccca ggccccggac ggtgaccggc acctcgacgg
     1441 gcgcgcggct gttgagtttc accggcgtgc ccagctcgac ggccagcttg cggcccttcg
     1501 gatccagcgg aacatacatc aggccgagcg cgcgcctcgg cttgggcgtg gccgccgggt
     1561 cccggggctg gatcacgctg accagcacat aggcgccgcc gccccaggcc gcgtccgtct
     1621 ttagggtcac cgatccgccg ttggggccga ccgagaaggt cttgaagtcg atgacccggt
     1681 cggtggccac ggccacctgg gcctcgcccg cgtagggcgc cttgatggtc agggtgacgg
     1741 agtcgccctg ggcgtggacc ccggtcccgg cgctgatgcg gacgaagtcc ggcgcctcgg
     1801 cgtccttggc cggggcgccc cagccggcgg cgaagcggat cacgctcttg gccccgccgg
     1861 ggccctcgac gaccacgcgg tagtcgcccc agccgagtcg ccgggcgatc cgcgccgcgc
     1921 cgccggcgcc gatgttcagg gcgcccttct ggaccaccac gtcgcggctg gtgcgccgcc
     1981 actgccagcg gccgtcctgc tggaaccagt cgtagtccca gttttcggcg atcagggtat
     2041 agctcgcccc gggggcggcg atccgctcgc cctgcgcgtt gacggcgatg aagtcgagcg
     2101 tcaccggggc cgcggacccg ccggcggcgt cggtctgttc aaccttcacc ccgaggtaga
     2161 cggggcgagt gcggactttg agaaacagac tctcgcgcac gggacggccg cccggttcga
     2221 acaccgacgc ggtcaccgtg gccttcaacg gcgcggtggt gtcgccggcc tcggacgcgg
     2281 cgacggacag caccgcccgg ccctcgccgt cggtcaccga cgagcccagt tcgatgaact
     2341 tctcggcgaa cgtcgtctgc tggtcgcccc actcatagcc cttgaaggcc gggaaggggt
     2401 tcggatcggc catgatccgc gcctcgccct gggtctgcag accggcgccg gccgcgccat
     2461 agaggaagcg ggccatgacg gagacctggc gagtctcgcc ggccgccagt ggcgtggtct
     2521 cccgtccctc ggtgctaacg gccagccgct gcggcgcgaa atcctcgacc gagaaggcca
     2581 tctccccggc cgccgccttg acgccctcga tctcgagcga cgccttccac cgtccgcggg
     2641 gcgcgctcct gggcaggacg acgtcggcgg tcacggcgcc ggtgggcgcg cggtcgaagg
     2701 ccacccgttt gaactcgacg cccgagggcc ggcggatgat cagcacgccc ttgcggtcct
     2761 tgaccgccct cgactgcaga tcgcggatca gggccgacag atggaccgtc tcgccgggac
     2821 ggtagatgcc ccggtccgca tagaggaagc cgtcgatctc gctgtcggcc acccggccgc
     2881 cggtctcgtc gtcgtcggcg gcgggcgcgt tgcggccgcc catgccctgt ttcgacaggt
     2941 cgaccggcga gcggtcgagg tcgagcacgg ccagatcgcc ctgggcgccg taggccatga
     3001 ccatcctggg atgcagggct ccgtcgccct cgagcaaggg tcgcaggaac cggacgcggc
     3061 cggcgctgtc ggtgcgggcg tcggcgaggg tctcgccatt cttggccacc agggccaccc
     3121 gcgtgcccgg cagcacccgc gcgctcttca gcgaccggac caccacatcg agcgactcgg
     3181 cgccgtcgta ggcgatcatc gccatgtcgg tgaacacgat ccagcgtcgc gcctgggcgg
     3241 gctgcacgcc ttcgctttcc tcgtcctggt cgtcggcggc cgtgggcctg gacgaggcgt
     3301 cgcgcgcctt gatcacatag ccgccgggct gcatgtcctt gagcaccgcg ccgagcggga
     3361 agaccgtggt cacccgctcg ccggcgccgc cgcgcacggc cacgtcgccc ttccagacca
     3421 cccggccctc gtcgttggga ctgtcgtcgc catagtcgcc cggccagtcg ccctcgccgg
     3481 tcgggtccgg cgcgctgatc gaggtgcgca ccaggttgcg gtcggcgacg cgccagacct
     3541 cgatcgacag ccgggtgacg ttgaccgtct cgatgccgac gccgtcggcg tcctcgcgcg
     3601 gcaggatcac gccgtcgccg gcgaagccga catagggcgg cttctcgccg aaggtgaaat
     3661 cgacgtcggc attggccgcc agggtctggc cgcccgcgcc cggcaggccc ttgagcaggg
     3721 tcacgcgccg ttcggtcagg cccaggccgc cgatgcacag ctcggccctg ttcttctggt
     3781 tgacgctgac cgccggcgtg ccgccgaggt cgggcgagac cagcacatag tccgcatagg
     3841 ccttgcccgg gtcgagcggc cgggtcatct cgatacaggc cagcggatcg gccttgctgg
     3901 tgtcgagccg cgtcttccag acggcgaatc cgtccggctt ggggatgccc cggcggggcg
     3961 cgccggcggc gcggggcttg ccgaacatcg accacagcga cggcttctcg gtcgggcccg
     4021 aggcgaccga accggccggt cccccgccct tgcccccgaa gccgccgccg agcaccgagg
     4081 cggtcaccag acccccgccg aaccccgccg ccagg
//
LOCUS       DAORNX010000269         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16276_length_4108_cov_7.819640, whole genome shotgun sequence.
ACCESSION   DAORNX010000269 DAORNX010000000
VERSION     DAORNX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_4108_cov_7.819640"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    220..336
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(371..1450)
                     /locus_tag="PLO65_11530"
     CDS             complement(371..1450)
                     /locus_tag="PLO65_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD28922.1"
                     /translation="MAFGAETIIQGDCIEQLRKLPDKSVDLVFADPPYNLQLGGDLLR
                     PDNSKVDAVDDHWDQFESFRAYDDFTRAWMAECQRVLKDDGALWVIGSYHNIFRVGTV
                     LQDLGFWILNDVVWRKSNPMPNFKGTRFANAHETLIWAAKSRGAKRYTFNYDAMKMAN
                     DEVQMRSDWTLPLCTGEERIKGADGHKAHPTQKPEALLYRVILTSTRPGDVILDPFFG
                     VGTTGAAAKRLGRRFIGIERESEYIAHAKERIRKVVPVSPEDLEVMGSKKSEPRVPFG
                     AVVEAGLLRPGDTVWCPKGQRAAKVRADGSLAVGDLTGSIHKVGAMIQSQPACNGWTY
                     WHFKSDKGLAPIDVLRSKVRAEMAA"
     gene            1613..1972
                     /locus_tag="PLO65_11535"
     CDS             1613..1972
                     /locus_tag="PLO65_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HRD28923.1"
                     /translation="MPYVDGFILAVKKNRIDDYKTMAALGGEVWMELGALSYVECFAD
                     DTPYGELTSFPRAVQAEDDEIVVFSWITYESREKRDEINAKVMADPRLQQDLATMPFD
                     GKRMIFGGFQSFIARGG"
     gene            complement(1969..2832)
                     /locus_tag="PLO65_11540"
     CDS             complement(1969..2832)
                     /locus_tag="PLO65_11540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD28924.1"
                     /translation="MCDDDIHVGLLVDPAVSRRTFGLMTLAAAGAAGSARAAVPVVET
                     DVMVKTPDGEADAAFFYPEGNGKWPAVLLWTDVAALRPAFREMGRRLAGEGYAVLVPN
                     VYYRSKRAPVISAGFDFNKPEDRAMLMAMRATLTPEAVDRDAAAYVAFLDARPQTDLG
                     RKVGTQGYCMGGALAFRTAAVAPGRVAAVGSFHGGGLYTDKPDSPHLLIARTRAEYLV
                     LIAGNDHQQDPTIEGKLRSAFGDANRPARVEVYESVKHGWCVPGSAVYDEPAAEKAWT
                     ELLAMYRRALT"
     gene            2943..>4108
                     /locus_tag="PLO65_11545"
     CDS             2943..>4108
                     /locus_tag="PLO65_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28925.1"
                     /translation="MTSPLAPLDDSRTSPFYEPHHRAWRDTLRRWVEAEIMPFVNDWD
                     EAGEFPRELYRKASEIGLLRLGWPEEYGGVATDPFMSIVTSQEMARHGAGGVNASLMS
                     HGIGLPPIRRMGSQEMKDRIIPQVLSGEKISALAITEPTGGSDVANLKTTARRDGDEW
                     VVNGSKMYITSGMRADFYTVAVRTGGEGAGGVSLLLIEREREGFSRQPLKKQGWWASD
                     TAQLFFDDVRVPADNLIGQENMGFSGIMQNFNGERIGMTAGAIGFSRVCLEDAIDWAR
                     ERKTFGKRLADHQVIRHKLADMYKRINATQAWLETLAWRIQNGESPIAEIALLKVQAT
                     ETMEYCAREAMQIMGGAGYMRGSRVERIYREVRVNAIGGGSEEIMRDLAARQMG"
BASE COUNT          652 a         1320 c         1381 g          638 t
ORIGIN      
        1 caacccgcgg cgcatcccac tccaccatgc tgcgcatggt ccccctctcc caagtgggag
       61 aggaaagtgt gaggcgcacc tcgccctgat gtcttgacgg ccagtgttgc tccggcgggg
      121 tcttggtcaa ggacgacccc tgcggggtcg ccgctgcgcg gccgcgaagc ggtccttgag
      181 caagggcccg tccggagcgg accattccat caaggccttn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg acgagcgcga ggatgacgga
      361 atgaggagaa ttaagccgcc atctccgccc ggaccttgct cctcagcaca tcgatcggcg
      421 ccaggccctt gtcagacttg aaatgccagt aggtccagcc gttgcaggcg ggctgggact
      481 ggatcatggc gccgaccttg tggatcgagc cggtcaggtc gccgacggcc aggctgccgt
      541 cggcgcggac cttggccgcg cgctggccct tggggcacca gacggtgtcg cccgggcgca
      601 gcaggccggc ctcgacgacg gcgccgaacg gcacccgcgg ctcggacttc ttgctgccca
      661 tcacctcaag gtcttccggc gacaccggca cgaccttgcg gatgcgctcc ttcgcatggg
      721 cgatgtattc gctctcgcgc tcgatgccga tgaaccggcg gcccagccgc ttggccgccg
      781 cgccggtagt gccgacgccg aagaacgggt cgaggatcac gtcgccgggg cgggtcgagg
      841 tcaggatcac gcggtagagc agggcctcag gcttctgcgt cggatgggcc ttgtggccgt
      901 cggcgccctt gatgcgctct tcgcccgtgc acagcggcag ggtccagtcg gagcgcatct
      961 gcacctcgtc gttggccatc ttcatggcgt cgtagttgaa ggtgtagcgc ttggcgccgc
     1021 ggctcttggc cgcccagatc agggtctcgt gggcgttggc gaagcgggtg cccttgaagt
     1081 tcggcatcgg gttggacttg cgccagacca cgtcgttgag gatccagaag ccgaggtcct
     1141 gcagcaccgt gccgacgcgg aagatgttgt ggtagctgcc gatgacccac agggcgccgt
     1201 cgtccttgag gacgcgctgg cactcggcca tccaggcgcg ggtgaagtcg tcgtaggccc
     1261 tgaagctctc gaactggtcc cagtggtcgt cgaccgcgtc gaccttggaa ttgtccggcc
     1321 gcagcaggtc gccgccgagc tgcagattgt agggcgggtc ggcgaacacc agatcgaccg
     1381 acttgtccgg caacttgcgc aactgctcga tgcaatcgcc ctggatgatg gtttccgcgc
     1441 cgaacgccat gtctgaacaa gcccctgaac ccgagagagt ccaaggaatc tggcggcgtt
     1501 acggttaagg gggcgttcgt cgacgtggtt agcgggagat taacaggggg tgtgtcggtt
     1561 cctgtcgccc gcgttcgtcc tgtggtcagg tagcgcccaa aggagagacc ccatgcccta
     1621 tgtcgatgga tttatcctcg cggttaagaa gaaccggatc gatgactaca agaccatggc
     1681 cgcgctgggc ggcgaggtct ggatggagct gggcgcgctc agctacgtcg agtgcttcgc
     1741 cgacgacacg ccctacggcg agctgacgtc ctttcctcgc gcggtgcagg cggaggacga
     1801 cgagatcgtc gtcttctcct ggatcaccta cgagtcccgc gaaaagcgcg acgagatcaa
     1861 cgccaaggtc atggccgatc cgcgcctgca acaggatctt gccaccatgc cgttcgacgg
     1921 caagcggatg atcttcggcg gattccagag cttcatcgcg cgcggcggct aggtcagcgc
     1981 ccgccggtac atcgccagca attcggtcca ggccttctcg gccgccggct cgtcgtagac
     2041 ggcgctgccg ggcacgcacc agccgtgctt gacggactcg tagacctcga ccctggccgg
     2101 gcggttcgcg tcgccaaagg ctgaccgcag tttgccctcg atggtcggat cctgctgatg
     2161 gtcgttgccg gcgatcagca ccaggtattc ggcgcgggtc ctggcgatca acaggtgggg
     2221 gctgtcgggc ttgtcggtgt agaggccgcc gccgtggaaa cttcccaccg ccgcgacccg
     2281 gcccggcgcc acggcggccg tgcggaacgc cagcgcgccg cccatgcaat agccctgtgt
     2341 gccgaccttc cttccgaggt cggtctgggg ccgggcgtcg agaaaggcca cataggcggc
     2401 ggcgtcgcgg tccaccgcct cgggcgtcag cgtcgcccgc atggccatca gcatggcccg
     2461 gtcctcgggc ttgttgaagt cgaagcccgc cgaaatcacc ggggcccgct tgctgcggta
     2521 atagacgttg ggaaccagca ccgcgtagcc ttcgccggcc aggcgccggc ccatctcgcg
     2581 aaacgccggc cgcagggcgg cgacgtcggt ccacagcagc accgccggcc acttgccgtt
     2641 gccctcggga tagaagaacg ccgcgtcggc ctcgccgtcg ggcgtcttca ccatcacatc
     2701 ggtctcgacc accgggacgg cggcgcgcgc ggacccggct gcgccggcgg cggccagggt
     2761 catcagtcca aaggtccggc gcgacacggc cggatcgacc agcaggccga cgtgaatgtc
     2821 gtcatcacac atgggtaggc tcctgtcggg ggcggtcgag gtctctcgac tgccgctgcg
     2881 gaatggctga caccatcgtc tcaaagcgct cgaacgagaa gcgttgccgg atcggagaca
     2941 tcatgaccag cccgctcgcc ccgctcgacg acagccgcac cagcccgttc tacgagccgc
     3001 accaccgcgc ctggcgcgac accctgcggc gctgggtcga ggccgagatc atgcccttcg
     3061 tcaacgactg ggacgaggcc ggcgagttcc cgcgcgaact gtaccgcaag gcctcggaga
     3121 tcggcctgct gcggctgggc tggcccgagg agtacggcgg cgtggccacc gacccgttca
     3181 tgtcgatcgt caccagccag gagatggccc gccatggcgc gggcggggtc aacgccagcc
     3241 tgatgagcca cggcatcggc ctgccgccga tccgccgcat gggcagccag gagatgaagg
     3301 accggatcat cccgcaggtg ctgtcgggcg agaagatctc cgccctggcc atcaccgagc
     3361 cgaccggcgg ctccgacgtg gccaacctga agaccaccgc ccgccgcgac ggcgatgagt
     3421 gggtggtcaa cggctccaag atgtacatca ccagcggcat gcgggccgac ttctacaccg
     3481 tcgccgtgcg cacgggtggg gagggggccg gcggggtctc cctgctgctg atcgagcggg
     3541 agcgcgaggg tttcagccgc cagccgctga agaagcaggg ctggtgggcg tccgacacgg
     3601 cccagctgtt cttcgacgat gtgcgggtgc cggccgacaa cctgatcggc caggagaaca
     3661 tgggcttctc cggcatcatg cagaacttca acggcgagcg gatcggcatg accgccgggg
     3721 cgatcggctt ctcgcgggtc tgtctcgagg acgccatcga ctgggcccgc gagcgcaaga
     3781 cgttcggcaa gcggctcgcc gaccaccagg tgatccgcca caagctggcc gacatgtaca
     3841 agcgcatcaa cgccacccag gcctggctgg agaccctggc ctggcggatc cagaacggcg
     3901 agtcgccgat cgccgagatc gccctgctca aggtccaggc caccgagacc atggagtact
     3961 gcgcccgcga ggcgatgcag atcatgggcg gggcgggcta catgcgtggg tcgcgggtcg
     4021 agcggatcta tcgcgaggtg cgggtcaacg cgatcggcgg cgggtcggag gagatcatgc
     4081 gcgacctggc ggcccggcag atggggat
//
LOCUS       DAORNX010000270         4104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16293_length_4104_cov_7.764633, whole genome shotgun sequence.
ACCESSION   DAORNX010000270 DAORNX010000000
VERSION     DAORNX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16293_length_4104_cov_7.764633"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /gene="polA"
                     /locus_tag="PLO65_11550"
     CDS             <1..1934
                     /gene="polA"
                     /locus_tag="PLO65_11550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRD28926.1"
                     /translation="MGAPRPAPAGGYSAAERPAAAPVETEHFDTSRYVCVRDLPTLQA
                     WVDRAREIGVVAFDTETDALGSAVAGLCGVSLAVAPGEACYIPLGHCEKDEGLFGGEG
                     GADLAQIPLDQALAVLRPLLEDPAVLKVAQNAKYDIAVLSRYGIQVAPIDDTMLISSV
                     LEAGLHGHGMDELSELWLGHKPIPFKQVCGTGKSEISFRHVALEPATAYAAEDADVTL
                     RLWRLLRPRLAREGLLTVYETTERPLPAVLAQMENHGVKVDPDLLRGLSHEFSMRMVD
                     LEAKAHELAGRPFNLGSPKQIGDILFGELGLEGGKKTATGQWATDVDVLERLAASHDL
                     PRTLLDWRQLSKLKGTYTDNLIAAINAKTGRVHTSYSLAAANTGRLASTDPNLQNIPI
                     RTEEGRKIRKAFIAEPGNVLISADYSQIELRLLAHVGDIPQLKRAFKEGLDIHAMTAS
                     EMFDTPIEGMPADVRRRAKAINFGIVYGISAFGLANQLGIDQSEAGAYIKTYFERFPG
                     IREYMDRMRAEVREKAYVTTLFGRKINIPDIRGKAVGMRQFAERAAINAPLQGAAADI
                     IRRAMAHMPSALTEAGLSSRMLLQVHDELVFEASLAEADETMRVAKAVMEKAALPAVD
                     LSVPLVVEARAAANWDEAH"
     gene            2499..3452
                     /locus_tag="PLO65_11555"
     CDS             2499..3452
                     /locus_tag="PLO65_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRD28927.1"
                     /translation="MPSLSRAPVAPVRDWYCDSAHWEQFTPREGDVVIATAPKVGTTW
                     TQQIVSLLVFQDPEPRHLGSLSPWLDCRFQIPLPVALQLLEAQTHKRFVKSHLPMSAL
                     PLWDEVKYIHVARDGRDACMSYLNHINGYTPLVWERLDATGEADPLLPGPKPRPPRDT
                     RGFFNHWIEKGGTRRAELMADAFFEIGRSFWAERMRPNVLLLHYNDMKADLDGEMRRI
                     AAFLDIAVDETVWPDLVAAAEFSAMQRNGDTLMAGVDVAFDGGHRTFLNKGTNGRWQG
                     ELTDADLALYRARVEAELSPGLAHWLEHGRRVAGDPRAAAD"
     gene            complement(3474..3869)
                     /locus_tag="PLO65_11560"
     CDS             complement(3474..3869)
                     /locus_tag="PLO65_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD28928.1"
                     /translation="MKGFVADIEDLTEDNRDFRRVLYTGKHLQLVLMALKPGEDIGEE
                     VHADHDQFFRIEDGEGEVWIDGERTPIRSDDAVIVPAGARHNVINTGDEPLKLYTIYG
                     PPDHRDGVIHATKAAAEAGEEHFDGRTTE"
BASE COUNT          649 a         1475 c         1381 g          599 t
ORIGIN      
        1 agatgggcgc gccgaggccc gccccggccg ggggttacag cgcggccgag cgcccggcgg
       61 cagcgccggt cgagaccgag catttcgaca ccagccggta cgtctgcgtg cgtgacctgc
      121 cgacactgca ggcctgggtc gatcgcgcgc gagagatcgg cgtggtcgcc ttcgacaccg
      181 agaccgacgc gctcggctcg gccgtcgcgg gtctctgcgg cgtgtcgctg gccgtggcgc
      241 cgggcgaggc ctgctacatc ccgctcggcc attgcgagaa ggacgagggg ctgttcggcg
      301 gcgagggcgg cgccgacctg gcgcagatcc ccctcgacca ggccctcgcc gtcctcaggc
      361 cgctgctgga ggacccggcg gtgctgaagg tggcgcagaa cgccaagtac gacatcgccg
      421 tgctcagccg ctacggcatc caggtcgcgc cgatcgacga caccatgctg atcagctcgg
      481 tgctcgaggc cggcctgcac gggcatggca tggacgagct cagcgagctg tggctcggcc
      541 acaagccgat cccgttcaag caggtctgcg gcacgggcaa gagcgagatc agcttcaggc
      601 acgtcgcgct ggagccggcc accgcctacg cggccgagga cgccgacgtg acccttcggc
      661 tttggcggct cctgcgcccg cggctggccc gcgagggcct gctgaccgtc tacgagacca
      721 ccgagcggcc cctgcccgcg gtgctggccc agatggagaa ccacggcgtc aaggtcgacc
      781 ccgacctgct gcgcggcctg agccatgagt tctccatgcg catggtcgac ctcgaggcga
      841 aggcgcacga gctggccggg cggccgttca acctgggcag ccccaagcag atcggcgaca
      901 tcctgttcgg cgagctcggc ctggagggcg gcaagaagac cgccaccggc cagtgggcca
      961 ccgacgtgga cgtgctcgag cggctggccg ccagccatga cctgccccgc accctgctcg
     1021 actggcgcca gctgtcgaag ctcaagggca cctacaccga caacctgatc gccgcgatca
     1081 acgcgaagac gggccgggtg cacacctcct acagcctggc ggcggccaac accggccgcc
     1141 tggcctcgac cgacccgaac ctgcagaaca tcccgatccg caccgaggaa ggccgcaaga
     1201 tccgcaaggc cttcatcgcc gagccgggca acgtgctgat cagcgccgac tacagccaga
     1261 tcgagctgcg gctgctggcc catgtcggcg acattcccca gctcaagcgg gccttcaagg
     1321 aggggctgga catccacgcc atgaccgcct cggagatgtt cgacaccccg atcgagggca
     1381 tgccggcgga cgtgcgccgc cgggccaagg cgatcaactt cggcatcgtc tacggcatca
     1441 gcgcctttgg cctggccaac cagctgggca tcgaccagtc ggaggccggg gcctacatca
     1501 agacctattt cgagcgcttc ccgggcatcc gcgagtacat ggaccggatg cgcgccgagg
     1561 tgcgcgagaa ggcctatgtg accaccctgt tcggccgcaa gatcaacatc ccggacatcc
     1621 gcggcaaggc cgtcggcatg cgccagttcg ccgagcgcgc ggcgatcaac gcgcctctac
     1681 agggggcggc ggcggacatt atccggcgcg ccatggcgca tatgccatcg gccctgaccg
     1741 aggccgggct gtcgtcacgc atgctgctgc aggtgcacga cgaactggtg ttcgaggcgt
     1801 ccctggccga ggccgacgag accatgcggg tggcgaaggc ggtgatggaa aaggccgccc
     1861 tgccggcggt cgacctgtcg gtcccgctgg tggtcgaggc ccgcgccgcg gccaactggg
     1921 acgaggcgca ttagggccaa tcgacattca gcgatcaaga ggccatcaaa ccccgattgt
     1981 ccccgcgaag gggaccatgc gaagcatggt ggagggagag gcgctggcgc tcgccgacca
     2041 ctgcgtctcc ctctccgacc cggattcgcc gggccacctc tccctccggg agaggacggc
     2101 cgaacgccca caccccgccc tgaatgccat ggcggcgagc ttgctccggt cttcggacgg
     2161 gtcaaggacc gtttcgcggc cgcgcagcgc cgggcccgcc gggcccgtcc ttgacgcgac
     2221 cggagaccgg agctcccatt ccacctaggc cttgagggct ttcccggcat ccaggaggac
     2281 gcccgtctca ctgtcatccc ggaaggcgcg cagcggctat ccgggaccca gattgaatcc
     2341 cacccgtcgt ccgtgaagcc gactctgggt cccggctctc cgcgcttcgc gctgcggccg
     2401 ggatgaccgt gagatgggtg gcttgacccc aaggcccctt cgcctaacgt cgctcgccac
     2461 aggccgccgc agcggcccgt ccggggggac acacagccat gccgagcctc agccgcgcgc
     2521 ccgtcgcgcc cgttcgcgac tggtattgcg actccgccca ctgggagcag ttcaccccgc
     2581 gcgagggcga tgtggtgatc gccaccgcgc ccaaggtcgg caccacctgg acgcagcaga
     2641 tcgtcagcct gctggtgttc caggaccccg agccgcgtca tctgggctcg ctgtcgccgt
     2701 ggctggactg ccgcttccag ataccgctgc cggtggcgct gcagctgctg gaggcccaga
     2761 cccacaagcg cttcgtcaag tcccacctgc cgatgagcgc cctgccgctg tgggatgagg
     2821 tgaaatacat ccacgtggcc cgcgacgggc gcgacgcctg catgtcctac ctgaaccaca
     2881 tcaacggcta cacgccgctc gtctgggaaa ggctcgacgc cacgggcgag gccgatcccc
     2941 tgctgcccgg tcccaagccc aggccgcccc gcgacacgcg cggcttcttc aaccactgga
     3001 tcgagaaagg cggcacgcgc agggccgagc tgatggccga cgccttcttc gagatcggac
     3061 ggtcgttctg ggccgagcgg atgcggccca atgtgctgct gctgcactac aacgacatga
     3121 aggccgacct cgacggggag atgcgccgca tcgcggcctt cctcgacatt gccgttgacg
     3181 agacggtctg gcccgacctc gtcgcggcgg ccgaattcag cgccatgcag cgcaacggcg
     3241 acaccctgat ggccggcgtc gacgtggcct tcgacggcgg ccaccggacc ttcctcaaca
     3301 agggcaccaa cggccgctgg cagggcgaac tgaccgacgc ggacctggcc ctctaccggg
     3361 ctcgcgtcga ggcggagctg tcgcctggcc tggcccactg gctcgagcac gggcggcggg
     3421 tcgccggcga cccgcgcgcc gcggccgact gaccccgtcg gtccgggccg cgcctactcg
     3481 gtcgtccggc cgtcgaaatg ctcctcgccg gcctcggccg ccgccttggt cgcgtggatc
     3541 acgccgtccc gatgatcggg cgggccatag atcgtgtaga gcttgagcgg ctcgtcgccg
     3601 gtgttgatca cgttgtgccg ggcgccggcg ggcacgatga cggcgtcatc gctgcggatc
     3661 ggggtgcgtt ccccgtcgat ccagacctcg ccctcgccgt cctcgatgcg gaaaaactgg
     3721 tcgtggtcgg catggacctc ctcgccgatg tcctcgccgg gcttgagcgc catcagcacc
     3781 agttgcaggt gcttgccggt gtagagcacg cgccggaagt cccggttgtc ctcggtcagg
     3841 tcctcgatgt ccgcgacaaa gcccttcatg ggtcgtcctt tccatggctc ctggcgccgc
     3901 cgtcgtggcg tcggcctgag aacgcatgag acgccggaac cgatcggccg gccttgctat
     3961 ggatcaagag cgacagggtt gcgccccgcg aatcgggaaa ttccgcgccg gcccggcctc
     4021 ggccgcgacc gctcctggcg atcaaatcct ggtggagccg aggggaatcg aacccctgac
     4081 ctcctcattg cgaacgaggc gctc
//
LOCUS       DAORNX010000271         4084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16397_length_4084_cov_7.457434, whole genome shotgun sequence.
ACCESSION   DAORNX010000271 DAORNX010000000
VERSION     DAORNX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16397_length_4084_cov_7.457434"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /gene="uvrA"
                     /locus_tag="PLO65_11565"
     CDS             complement(<1..2342)
                     /gene="uvrA"
                     /locus_tag="PLO65_11565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRD28929.1"
                     /translation="MADHQNFIRVRGAREHNLKNVSVDIPRGELVVITGLSGSGKSSL
                     AFDTIYAEGQRRYVESLSAYARQFLELMSKPDVDLIEGLSPAISIEQKTTSRNPRSTV
                     GTVTEIHDYMRLLWARVGVPYSPATGLPIESQTVSQMVDKLTALPEGERLYLLAPVVR
                     GRKGEYRKEIAEWRKLGFQRLKIDGVLYPIEDAPELDKKFKHDIDVVVDRLVTKTGLE
                     QRYADSIEQALRLADGIAVAEWADVAEGEAEPRRILFSEKFACPVSGFTISEIEPRLF
                     SFNNPFGACPTCDGLGAKLAFDADLVVPDKDKTLHKGAVSPWAKGPSPLYTQTLQALA
                     RHYGFSMDLPWHALPEQARQVVLYGTGSEKIHFIYDDNARKYEVNKPFEGVLPNLERR
                     WRETDSSWVREELGRYQSDTPCDVCHGARLKPEALAVKVGERNIAEVSALAIRQAREW
                     FEELEPRLTDKQMEIARRILKEINDRLRFLVDVGLDYLNLSRGSGSLSGGESQRIRLA
                     SQIGSGLTGVLYVLDEPSIGLHQRDNTRLLQSLRGLRDLGNSVLVVEHDEEAILTADY
                     VIDMGPAAGIHGGHVVAQGKPEDILTNGNSVTGQYLSGMREIPVPEDRRPISTKNVLR
                     VVGATGNNLKSVTAEIPVGTFTCITGVSGGGKSTFTIETLYKAAARRLNNASDAPAPY
                     ERIEGLEHFDKVIDIDQSPIGRTPRSNPATYTGAFQPIRDWFSQLPESKARGYGPGRF
                     SFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCDVCKGKRY"
     gene            2574..>4084
                     /locus_tag="PLO65_11570"
     CDS             2574..>4084
                     /locus_tag="PLO65_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD28930.1"
                     /translation="MPRFAPTTRLETHEVFNQPPAFAGRNLFDSDLALREAAIHEGGD
                     WVASPLTALGRDAGSEAVLELGDLANRFPPELVTFDRYGRRIDEARFHPSYHQLMGLA
                     MDHHIHDIAWTAKRPGGHVAHAGLLALMTEAEAGVLCPVSMTYAAVPALRHQPDVTGP
                     WLEKLIGGTYDGPLAPIAGKRGITVGMAMTEKQGGSDVRANTTRAHPLGGGLYRLVGH
                     KWFCSAPMSDGFLTLAYAEGGLSCFLVPRIAPDGSRNGIQVMRLKDKLGNKSNASSEI
                     EYHDATAWLLGEEGKGVSVIIDMVHHTRLDTMAGTLGIMRRPLAEAVNHVTGRRVFQK
                     TLADQPAMRAVIADLGLEYEAAAVMTMRVARAFDGGEEGDRAFARLAVALGKYWLTKR
                     CPNFVYECMECHGGVGYVEETPLPRYFRESPLNAIWEGSGNVIALDVLRTLRKEPAAM
                     DSFVAEVALARGADARLDAAAARLADRFAGPVGEGDVRRLVEEDSSKPRYIQTV"
BASE COUNT          599 a         1391 c         1424 g          670 t
ORIGIN      
        1 ttgtagcgct tgcccttgca gacgtcgcag gtgacgtaga cgtcgggcag gaagtgcatc
       61 tcgatcttga tcagcccgtc gccctggcag gcctcgcagc ggccgccctt gacgttgaag
      121 ctgaaccggc ccgggccgta gccgcgcgcc ttgctctccg gcagctggct gaaccagtcg
      181 cggatcggct ggaaggcgcc ggtgtaggtc gccggattcg agcgcggcgt gcgtccgatc
      241 gggctctggt cgatgtcgat gaccttgtcg aaatgctcca gcccctcgat ccgctcgtag
      301 ggcgccgggg cgtcgctggc gttgttcagc cgccgggccg cggccttgta gagggtctcg
      361 atggtgaagg tcgacttgcc gccgccgctc acgccggtga tgcaggtgaa ggtcccgacc
      421 gggatctctg cggtcacgct cttgagattg ttgccggtcg ccccgaccac gcgcagcacg
      481 ttcttcgtcg agatcggccg ccggtcctcc ggcaccggaa tctcgcgcat gcccgacagg
      541 tactggccgg tgacgctgtt gccgttggtc aggatgtcct cgggcttgcc ctgggcgacg
      601 acgtggccgc catggatgcc ggccgccggc cccatgtcga tgacatagtc ggcggtcagg
      661 atcgcctcct cgtcatgctc gacgacgagc accgagttgc cgaggtcgcg cagtccgcgc
      721 agcgactgca gcagccgcgt gttgtcgcgc tggtgcaggc cgatcgacgg ctcgtcgagc
      781 acgtagagca cgccggtcag gcccgagccg atctggctgg ccagccggat tcgctggctc
      841 tccccgccgc tcagcgaacc cgaaccgcgc gacaggttca gatagtccag gccgacgtcg
      901 acgaggaacc gcaggcgatc gttgatctcc ttcaggatcc gccgggcgat ctccatctgc
      961 ttgtcggtca gccggggttc gagttcctcg aaccattcgc gggcctggcg gatggccagg
     1021 gcgctgacct cggcgatgtt gcgctcgccg accttgacgg ccagggcctc tggcttgagg
     1081 cgggcgccgt ggcagacgtc gcacggcgtg tcggactgat agcggccgag ctcctcgcgc
     1141 acccagctgg agtccgtctc gcgccagcgc cgctcaaggt tgggcagcac gccctcgaac
     1201 ggcttgttga cctcgtactt gcgggcgttg tcgtcgtaga tgaagtggat cttctcgctg
     1261 cccgtgccgt acagcacgac ctgccgggcc tgctcgggca gggcgtgcca cggcaggtcc
     1321 atcgagaagc cgtaatggcg cgccagcgcc tgcagggtct gggtgtagag cggcgacggg
     1381 cccttggccc agggtgacac cgcccccttg tgcagggtct tgtccttgtc cggcaccacc
     1441 aggtcggcgt cgaacgccag cttggccccc aggccgtcac aggtcgggca ggcgccgaac
     1501 ggattgttga acgagaacag ccgcggctcg atctcgctga tggtgaagcc gctgaccggg
     1561 caggcgaact tctcggaaaa caggatccgc cgcggctcgg cctcgccctc ggcgacatcg
     1621 gcccattcgg ccaccgcgat gccgtcggcc aggcgcaggg cctgttcgat gctgtcggcg
     1681 tagcgttgct caaggccggt cttggtcacc agccggtcca cgaccacgtc gatgtcgtgc
     1741 ttgaacttct tgtccagttc gggggcgtcc tcgatcgggt agagcacgcc gtcgatcttc
     1801 agccgctgga agccgagctt gcgccactcg gcgatctcct tgcggtactc gcccttgcgg
     1861 ccgcgcacga cgggggccag caggtacagc cgctcgccct cgggcagggc ggtcagcttg
     1921 tcgaccatct ggctgacggt ctggctctcg atcggcaggc cggtggccgg cgaatagggc
     1981 actccgaccc gcgcccacag caggcgcatg tagtcgtgga tctcggtgac cgtgccgacc
     2041 gtcgagcgcg gattgcggct ggtggtcttc tgctcgatcg agatggccgg cgagaggccc
     2101 tcgatcaggt cgacgtccgg cttgctcatc agctcgagga actggcgggc gtaggccgac
     2161 aggctttcga cgtagcggcg ctggccctcg gcgtagatcg tgtcgaacgc cagcgacgac
     2221 ttgcccgagc ccgacaggcc ggtgatcacc accagctcgc cgcgcgggat gtcgacgctg
     2281 acgttcttga gattgtgctc gcgcgcgccg cgcacgcgga tgaagttctg atggtcggcc
     2341 atggtcaccg ggaggaacgc tcggccggcc tgttcggacg cggcgcaaca gccatatgta
     2401 gtgcgcagag tcgcctttaa cacaagaaca atgtgagaac gcgggggttc ccccgccgtc
     2461 ctgctgacat ggactgtcag caggacggcg gttgcggcgg cgcgcttgac gcgcatcatg
     2521 gcgcaccggc gcgtcccggc agcccctgac gcgccgctcg ccggaggacc attatgcccc
     2581 gcttcgcccc gaccacccgg ctcgagaccc acgaggtgtt caaccagccg ccggcgttcg
     2641 cggggcgcaa cctgttcgac agcgacctgg cccttcgcga ggcggcaatc cacgagggcg
     2701 gggactgggt ggcctcgccg ctgactgcgc tcggccgtga cgccggttcc gaagcggtgc
     2761 tggagctcgg cgacctggcc aaccgtttcc cgcccgagct ggtgaccttc gaccgctacg
     2821 gccggcggat cgacgaggcg cggttccatc cgagctacca ccagctcatg ggcctggcga
     2881 tggatcacca catccatgac atcgcctgga ccgccaaacg gcccggcggc cacgtcgccc
     2941 atgccgggct gctggccctg atgaccgagg ccgaggccgg ggtgctgtgc ccggtcagca
     3001 tgacctacgc ggccgtgccg gccctgcgcc atcagccgga cgtcacgggg ccgtggctcg
     3061 agaagctcat tggcggaacc tacgacggcc ccctcgcgcc gatcgccggc aagcgcggga
     3121 tcacggtcgg catggccatg accgagaagc agggcggcag cgacgtgagg gccaacacca
     3181 cccgcgccca cccgctgggc ggcgggctct accgccttgt cggccacaaa tggttctgct
     3241 cggccccgat gagcgatggc ttcctgaccc tggcctatgc cgagggcggc ctgagctgct
     3301 tccttgtgcc ccgcatcgcc ccggacgggt cgcgtaacgg catccaggtc atgcggctga
     3361 aggacaagct gggcaacaag tccaacgcct cgagcgagat cgagtaccac gacgccacgg
     3421 cctggctgct gggcgaagag ggcaagggcg tgtcggtgat catcgacatg gtccaccaca
     3481 cccggctgga caccatggcc gggacgctcg gcatcatgcg ccggccgctg gcagaggcgg
     3541 tcaaccatgt caccggccgg cgcgtgttcc agaagaccct ggccgaccag ccggccatgc
     3601 gggcggtgat cgccgacctg gggctggagt acgaggccgc cgccgtgatg accatgcgcg
     3661 tggcccgggc cttcgacggc ggggaggagg gcgaccgggc cttcgcccgc ctggccgtgg
     3721 cgctgggcaa gtactggctg accaagcgct gcccgaactt cgtctacgag tgcatggagt
     3781 gccacggcgg cgtcggctac gtcgaggaga cgcccctgcc gcgctatttc cgcgagagcc
     3841 ccttgaacgc catctgggag ggctcgggca acgtcatcgc gctggatgtg ctgcgcaccc
     3901 tgcgcaagga acccgccgcg atggacagct tcgttgccga ggtggccctg gcgcgcggcg
     3961 cggacgcccg gctggacgcc gccgccgcgc ggttggccga tcgtttcgcc ggcccggtcg
     4021 gggagggcga cgtgcgccgg ctggtcgagg aggactcatc gaagccgcgc tacattcaaa
     4081 ccgt
//
LOCUS       DAORNX010000272         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16413_length_4081_cov_7.151018, whole genome shotgun sequence.
ACCESSION   DAORNX010000272 DAORNX010000000
VERSION     DAORNX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16413_length_4081_cov_7.151018"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLO65_11575"
     CDS             <1..295
                     /locus_tag="PLO65_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD28931.1"
                     /translation="PASLSLGACAFDIERGELSRDGSPIRLTEAEVELMRRLARSANA
                     PVDRLELARDTADVTGRAVDVQVTRLRRKIESDPKNPRYLQTVRGVGYRLAPD"
     gene            295..1644
                     /locus_tag="PLO65_11580"
     CDS             295..1644
                     /locus_tag="PLO65_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD28932.1"
                     /translation="MPRLRALKIFIPRQIKRWLPTSLFGRSLLIIILPIALMQIAVTW
                     VFFDAHWQTVTSRLSDSLAGDISWAVESYQDDPSPESLARVAERAQDSMSLSIGLSPV
                     RTLPPRRPTSVFGALDRSLDRALSEHLETPYWFDTVRFPGYVDIRVKVGDQVMRIIAP
                     SDRAWATQGGIFVLWMTVATLLLTAIAILFIRNQVRAIERLASAAEAFGKGGDVADFK
                     PYGAREVRQAAQAFLAMKSRIQRHLEQRTAMLASVSHDLRTPLTRLKLELALAEPSRR
                     VEAMKTDLSEMEHMIDEYLAFARGEGGEAVEMTVLRDLIGEVSEGAARAGAEVAIDVD
                     AALVLAVRPNALKRALSNLVMNAAAHGEHIVVSARQTPAGGAEIIVDDDGPGIPPEQY
                     EEAFRPFNRLDESRNQNEKGVGLGLAIARDVARSHGGEMTLDRSPAGGLRAVIRLPG"
     gene            complement(1641..2480)
                     /locus_tag="PLO65_11585"
     CDS             complement(1641..2480)
                     /locus_tag="PLO65_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD28933.1"
                     /translation="MSCREAAGVPAGSPRISVAGEQIAVCREGRGVPVVCLTAIAHGG
                     GDFDAFAARMKSRFEIIRIDWPGHGRSGDAAAAPSARRYADLLAELLPALAVEHPILI
                     GNSIGGAAAILHAERHPVRGLVICNAGGLVAVDATVKRFCSIFAGFFAAGERRAGWFG
                     PAFAAYYRLLVLPSRSAAAQRRRIIASGYEVAGQLRLAWESFGRAEADIRDVAGRLDA
                     PVWVAWSRGDRIIPLGRCRPAIKAMKRAVLTTFAGGHAPFLEQPDAFAEAFVDFTDAL
                     RAA"
     gene            2548..3096
                     /locus_tag="PLO65_11590"
     CDS             2548..3096
                     /locus_tag="PLO65_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD28934.1"
                     /translation="MRPGKSPSSTREQLIVAAAAEFGEHGFGGTDTNRIARRAGFAPQ
                     TFYRWFKDKTEIFLAVYRAWEEAERETMTDLLRRGAGETEQIEAALADHRDHRVFRRS
                     LRALSLTDDAVRLARAQSRRRQIERIGGSPETAAVLLLQLERLADALAEGELADMGLD
                     DAVARQCLEDLLRQLRSNGKRG"
     gene            complement(3093..3884)
                     /gene="proC"
                     /locus_tag="PLO65_11595"
     CDS             complement(3093..3884)
                     /gene="proC"
                     /locus_tag="PLO65_11595"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRD28935.1"
                     /translation="MSRVLLLGAGRMGGALVSGWMFGGSVSVETLMIRDPFPGAEAKE
                     AVEQGARLNPADVDLMRADTVVLAVKPQLWRDAAAAVEPWLAHDAVIISVVAGVGLQD
                     LREAFGSRFVARVMPTIAVAVSQGTATVYSDDDGARDRAHLLFEGVAQVVDVDDEGLM
                     HAATAVSGSGPAYLYAFVEALEAAGLQAGLSADAAVTLARSTITGAASLMSLSDASPA
                     ELRRQVTSPGGTTQAALEVLMGEGGLQDLLDEAVAAAVRRSKELG"
     gene            complement(3897..>4081)
                     /locus_tag="PLO65_11600"
     CDS             complement(3897..>4081)
                     /locus_tag="PLO65_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28936.1"
                     /translation="RHALSLPGGERPTLAAAASMIDAAVEAADRFYPAFDFLLAGAKT
                     PDEAMAACMFETVGEA"
BASE COUNT          661 a         1441 c         1409 g          570 t
ORIGIN      
        1 ccccgccagc ctgtcgctgg gcgcctgcgc cttcgacatc gagcggggcg agctgtcgcg
       61 ggacgggtcg ccgatccgcc tgaccgaggc ggaggtcgag ctgatgcgcc gcctggcccg
      121 ctcggccaac gccccggtcg accggctgga gctcgcgcgc gataccgccg acgtgaccgg
      181 ccgcgccgtc gatgtccagg tcacccggct gcggcgcaag atcgagtccg atccgaaaaa
      241 cccccgctat ctgcagaccg tgcgcggcgt cggctaccgg ctggcgcccg actgatgccc
      301 cggctgcgcg cgctcaagat cttcatcccg cgccagatca agcggtggct gccgacgagc
      361 ctgttcggcc gcagcctgct gatcatcatc ctgccgatcg ccctgatgca gatcgccgtc
      421 acctgggtgt tcttcgacgc ccactggcag acggtgacca gccgactgtc ggacagcctg
      481 gccggcgaca tttcctgggc ggtcgagagc tatcaggacg atcccagccc cgagtccctg
      541 gcccgggtgg ccgaacgggc ccaggactcg atgtctctgt cgatcggcct ctcgcccgtc
      601 cggaccctgc cgccgcgccg gccgacgtcg gtgttcggcg ccctcgaccg gtcgctcgac
      661 cgggctctgt cggagcatct ggagacgccc tactggttcg atacggtccg ctttccgggc
      721 tatgtcgaca tccgcgtgaa ggtcggcgac caggtgatgc ggatcatcgc gccctccgac
      781 cgggcctggg ccacccaggg cggcatcttc gtgctgtgga tgaccgtcgc gaccctgctg
      841 ctgacggcca tcgccatcct gttcatccgc aaccaggtgc gggccatcga acggctggcc
      901 agcgcggcgg aggcgttcgg caagggtggc gatgtcgccg acttcaaacc ctatggcgcg
      961 cgcgaggtgc ggcaggcggc gcaggctttc ctggcgatga agagccgcat ccagcgccac
     1021 ctcgaacagc gcacggccat gctggcgtcg gtcagccacg acctgcgcac cccgctgacc
     1081 cgactgaagc tggaactggc cctggccgag ccgtccaggc gggtcgaggc gatgaagacc
     1141 gacctgtccg agatggagca tatgatcgac gagtacctcg ccttcgcccg cggcgagggc
     1201 ggcgaggcgg tcgagatgac ggtgctgcgc gacctgatcg gcgaggtcag cgagggcgcc
     1261 gcgcgggccg gggccgaggt ggcgatcgat gtcgacgccg cgctggtgct ggcggtgcgg
     1321 cccaacgcgc tgaagcgggc cctttcgaac ctggtgatga acgccgcggc gcacggcgag
     1381 cacatcgtcg tttccgcgcg ccagaccccg gccggcggcg ccgagatcat cgtcgacgac
     1441 gacggccccg gcatcccgcc cgagcagtac gaggaagcct ttcgcccgtt caaccggctc
     1501 gacgagtcgc gcaaccagaa cgaaaagggt gtcggcctgg gcctggccat cgcgcgcgac
     1561 gtggcgcgca gccacggcgg ggagatgacc ctagaccgct cgccggcggg cgggctgagg
     1621 gcggtgatcc gcctcccggg ctaggctgcg cgcagggcgt cggtgaagtc gacaaacgcc
     1681 tcggcaaagg cgtcaggctg ttcaagaaag ggcgcgtggc cgccggcaaa ggtcgtgagg
     1741 accgcccgct tcatcgcctt gatcgccggg cggcagcggc cgaggggaat gatccggtcc
     1801 cctcggctcc aggccaccca gaccggcgcg tcgagccgcc cggccacgtc gcggatgtcg
     1861 gcctcggccc tgccgaaact ctcccaggcc agtcgaagct ggccggccac ctcgtagccg
     1921 ctggcgatga tccgacggcg ctgcgcggct gccgaccgcg agggcaggac cagcagccgg
     1981 tagtaggccg cgaaggccgg accgaaccat ccggcccgcc gctcgccggc cgcgaagaag
     2041 ccggcgaaga tcgagcagaa ccgcttcacg gtcgcgtcca ccgccacgag gccgccagcg
     2101 ttgcagatca ccagcccgcg tacgggatgc cgctctgcat ggaggatggc cgccgccccg
     2161 ccgatagagt tgccgatgag aatcggatgt tcgaccgcga gggccggcag cagctccgcg
     2221 agaaggtccg cgtaccgtcg cgcgctcggc gccgccgccg cgtcgccaga ccgaccatgg
     2281 cctggccagt cgatgcggat gatctcgaac cgcgacttca tccgcgcggc gaaagcgtcg
     2341 aagtcgcctc ctccgtgggc gatggcggtc agacagacga cagggacgcc gcgcccctcg
     2401 cgacagaccg cgatctgctc tcccgcgaca ctgatccgtg gcgaccccgc agggaccccg
     2461 gcggcttctc gacaggacat ggagctctcc aagtgattag aatatcactt gtgatttaga
     2521 tatcacttcg gagtcaagga ggctctgatg cgcccgggaa agtcaccgtc gtccacacgg
     2581 gaacagctga tcgtcgcggc ggcggcggaa ttcggcgagc acggctttgg cggcaccgac
     2641 acaaatcgca tagcccgccg cgccggcttc gcgccccaga ccttctatcg ctggttcaag
     2701 gacaagacgg agatcttcct ggccgtctac agggcctggg aggaagcgga acgcgagacg
     2761 atgaccgacc tcctgcggcg cggcgcgggc gagacggaac agatcgaggc cgcgctggcc
     2821 gatcatcgcg atcatcgcgt cttccggcgc agcctgcggg cgttgtcgct gacggacgac
     2881 gcggtcaggc tcgccagagc tcaaagccgt cgacggcaga tcgagcggat tggcggttca
     2941 ccggagacgg cggcggtgct gctgcttcaa ctggagaggc tggcggacgc cctcgccgaa
     3001 ggcgaactgg ctgatatggg tctggatgac gctgtggcga ggcagtgcct ggaggacctg
     3061 ctccggcagc tgaggagcaa cggaaaacgc ggctagccca gttctttcga ccgccgcacg
     3121 gccgccgcca ccgcttcatc gagcaggtcc tgcaggccgc cctcgcccat cagcacctcg
     3181 agcgccgcct gggtcgtgcc gccgggtgag gtgacctgcc gtcggagctc cgcgggcgag
     3241 gcatcgctca ggctcatcag ggacgcggcg ccggtaatgg tcgagcgagc cagggtgacg
     3301 gcggcgtcgg ccgacaaacc tgcctggagt ccggctgctt cgagcgcctc gacgaaggcg
     3361 tagagatagg ccgggccgga ccccgacacg gccgtcgcgg catgcatcag gccttcgtca
     3421 tcgacgtcca ccacctgggc cacgccctcg aacagcagat gcgcccggtc gcgcgcgccg
     3481 tcatcgtcgg aataaacggt cgctgttccc tgcgagacgg ccaccgcgat ggtcggcatg
     3541 acgcgggcca cgaaacggct cccgaaggct tcgcgaaggt cctgaaggcc aacgccggcg
     3601 accacggaga tgatcaccgc gtcgtgggcc agccatggct caaccgccgc cgccgcatcc
     3661 cgccagagct ggggcttgac cgccagcacc accgtgtcgg cccgcatcag gtcgacgtcc
     3721 gccggattta gcctggcgcc ctgttcaacg gcttccttgg cctcggcgcc gggaaaagga
     3781 tccctgatca tcagggtctc gaccgacacg gacccgccga acatccagcc ggacaccagc
     3841 gcgccgccca tccgtccggc gccgagcagg agaacgcgtg acatgccaga acagccctag
     3901 gcttcgccga cggtctcgaa catgcaggcg gccatggcct cgtccggggt cttggcgccg
     3961 gccagcagga agtcgaacgc cggatagaac cggtcggccg cctccaccgc cgcgtcgatc
     4021 atcgacgccg ccgccgccag ggtcgggcgc tcgcccccgg gcaggctcag ggcgtggcgg
     4081 a
//
LOCUS       DAORNX010000273         4045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16602_length_4045_cov_7.776942, whole genome shotgun sequence.
ACCESSION   DAORNX010000273 DAORNX010000000
VERSION     DAORNX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16602_length_4045_cov_7.776942"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..657)
                     /locus_tag="PLO65_11605"
     CDS             complement(10..657)
                     /locus_tag="PLO65_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRD28937.1"
                     /translation="MDEDLDALVRRVDPDRWLSSRFVADAAARADLVALYALDHELVR
                     IPQVVSDPLMGEIRMTWWREGLEEIADGRRPRSHPVLRAIAASALPTLALAALAEARL
                     GDLDGPKTGEAALAHADATEGLLMALAARRLSPTATADHVRQAARAVALAKTDASAAL
                     VALRASRTELPALPVAAFPAVAHAALVGRHARGGAPGDLEKRLRILLAVLTGRIG"
     gene            complement(723..1328)
                     /locus_tag="PLO65_11610"
     CDS             complement(723..1328)
                     /locus_tag="PLO65_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRD28938.1"
                     /translation="MAGLLSNFRNTIILSVLLALVMIFVYFQHHGALDVTFWQAVLRW
                     AHTFSGILWIGLLYYFNFVQIRVMPDIPAELKPAVSKHIAPEALFWFRWAALATWVFG
                     VILAWNRGFLVEAFTLGASESFAVPSHIMIGIGMWLATIMFINVWGVIWPNQKIALGM
                     KEADADAKAKAAKTAMQFSRINTLLSLPMLTAMTMNQTLFG"
     gene            complement(1411..1764)
                     /locus_tag="PLO65_11615"
     CDS             complement(1411..1764)
                     /locus_tag="PLO65_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HRD28939.1"
                     /translation="MRQPPSIDAWGGGGFRVSGDWRPGSLLILDDVARPWSASVLADL
                     TPEDFAPAFAATGVVEFVLLGTGLNPGLPPRAVRDAIRAAGLGLEFMSTEAAARSYNV
                     LASEGRRMAAALLAV"
     gene            complement(1773..2750)
                     /gene="secF"
                     /locus_tag="PLO65_11620"
     CDS             complement(1773..2750)
                     /gene="secF"
                     /locus_tag="PLO65_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRD28940.1"
                     /translation="MSFWPLIKILPVKTNFRFVDFAKYAGALSIVATIASLALVLLPF
                     KPPCGGLTCGIDFKGGTVLEFTTYPKPVDLAAVRAAVSRQDLGDVGVQAFGEGANAGV
                     MVRFQNPPGKNANAVKSEVRGAIQQALGEVRFTNDATVGAKVSGELFTSGILALLVAI
                     ALMLVYIWFRFELQFGLGAVVALFHDVILTFGLIAFTKMEFSLTTIAAILTIIGYSMN
                     DTVVVFDRLRENLRKYKRMPLREVIDLSINETLSRTVITGLTAILALGGLAVFGGEAL
                     FGFSVIMMFGIALGTYSSIYVAAPVILLWGVKRGEEEATPIKFGAASRP"
     gene            complement(2762..>4045)
                     /gene="secD"
                     /locus_tag="PLO65_11625"
     CDS             complement(2762..>4045)
                     /gene="secD"
                     /locus_tag="PLO65_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRD28941.1"
                     /translation="DQAQVANAYNILSRLIQLTKTGKPDLKVTRDGDQRIGLAFSETA
                     LAEQASGAVDQSIEIIRRRVDELGTREPAITRQGTNRIVLQAPGESDPEKLKAVIGQT
                     AKLTFQMVDTTVSLQEAQAGRLPPGSEILPSTDRYAPAYVVRRRVDVSGDMLTNAAQV
                     NDPQTGEVQVSFRFNSQGARRFAEVTSRNIGKPFAIILDGKVISAPRINSAITGGSGV
                     ITGSYTVESANELAILLRAGALPAPLNVEEQRTVGAELGADAVEAGKISTIVGFTAIL
                     VFMLLAYGGLFGGISVIALLVNGLLIIAAMSATQATLTLPGIAGLILTLAVAVDANVL
                     IYERMRDEVRAGRPLVTAMDAGFSRAMETIIDANLTTLLAALIMFFFGAGPVRGFAWT
                     LSIGVFTSVFTAVVVTQLLLALWFRYARPKTLPIA"
BASE COUNT          694 a         1354 c         1405 g          592 t
ORIGIN      
        1 ccccggccgc tagccgatcc ggccggtcag cacggcgagc aggatgcgca gccgcttctc
       61 gaggtccccg ggcgcgccgc cgcgcgcatg gcgtccgacc agggctgcat gggccacggc
      121 ggggaaggcc gcgaccggca gggcaggcag ctccgtccgc gacgccctga gcgccaccag
      181 ggcggccgac gcgtcggtct tcgccagggc gaccgctcga gccgcctgtc ggacgtggtc
      241 agcggtcgcc gtgggtgaca ggcggcgggc cgccagggcc atcagcaggc cttcggtcgc
      301 gtcggcatgg gccagggcgg cctcgcctgt cttcgggccg tccagatcgc ccagtcgggc
      361 ctcagcgagc gcggccagcg ccagggtcgg tagcgcgctc gccgcgatcg cccgcaaaac
      421 ggggtgagac cggggccgtc ggccgtcggc gatctcctcc agaccttccc gccaccaggt
      481 catgcggatc tcgcccatca gcggatcgct gacgacctgc gggatgcgca ccagttcatg
      541 gtcgagggcg tagagggcga ccagatccgc gcgcgcggcc gcgtcggcca cgaagcggct
      601 ggacagccag cggtcggggt cgacccggcg gacaagggcg tcgagatctt cgtccatggc
      661 cttcaaacga agaggggccc gccgtcgggc aggcccctcc cctgtttcgt gctctcggcg
      721 gtctagccga acagcgtctg gttcatcgtc atcgcggtca gcatcggcag gctcagcagc
      781 gtgttgatgc gcgagaactg catcgccgtc ttcgcggcct tggccttggc gtccgcgtcc
      841 gcttccttca tgcccagcgc gatcttctgg ttcggccaga tcacgcccca gacgttgatg
      901 aacatgatcg tcgccagcca catgccgatg ccgatcatga tgtggctcgg gaccgcgaag
      961 ctctcgctgg cgccaagcgt gaaggcctcg accaggaagc cccggttcca ggcgagaatc
     1021 acgccgaaca cccaggtcgc cagggcggcc cagcggaacc agaacagcgc ctcgggcgcg
     1081 atgtgcttgg acacggccgg cttcagctcg gccggaatgt cgggcatcac ccggatctgg
     1141 acgaagttga agtaatacag caggccgatc cagaggatgc cgctgaaggt gtgggcccag
     1201 cgcagcacgg cctgccagaa ggtcacgtcc agcgcgccat ggtgctggaa gtagacaaag
     1261 atcatgacca gcgcgagaag cacgctgaga atgatggtgt tgcggaagtt ggagagaagt
     1321 ccggcgatca tcgaaaatgc ccctcagtag agtcctaccc aggcggtcag ctataggccg
     1381 cttcgccccg cccggtaaac agtctggcga tcacaccgca agcagggcgg ccgccatgcg
     1441 gcggccttcc gaagccagca cattgtagct ccgggccgcg gcctcggtgg acatgaactc
     1501 cagacccagc ccggcggccc ggatcgcgtc gcgcacggcc cgcggcggca ggcccgggtt
     1561 cagtccggtt ccgagcagaa cgaactcgac gacgccggtc gcggcgaacg ccggcgcgaa
     1621 gtcctcgggc gtcaggtccg ccagcaccga ggcgctccag ggcctggcca catcgtcgag
     1681 gatcagcagc gaaccgggcc gccagtcacc cgacacccga aatccgccgc cgccccaggc
     1741 gtcgatggac ggcggctggc gcaccgccgc gatcagggcc gcgaggccgc gccgaacttg
     1801 atcggcgtgg cctcttcctc gccgcgcttg acgccccaca gcaggatcac cggcgcggcg
     1861 acatagatcg acgaataggt gccaagggcg atgccgaaca tcatgatgac ggagaaaccg
     1921 aacagcgcct cgccaccgaa caccgccagg ccgccgaggg ccaggatcgc cgtcaggccg
     1981 gtgatcacgg tacgcgacag ggtctcgttg atcgacagat cgatcacctc ccgcagcggc
     2041 atcctcttgt acttgcgcag gttttcccgc agccggtcga acaccaccac cgtgtcgttc
     2101 atcgaatagc cgataatggt caggatcgcg gcgatggtcg tgaggctgaa ctccatcttg
     2161 gtgaaggcga tcaggccgaa ggtcaggatc acgtcgtgga acagggccac aaccgcgccc
     2221 aggccgaact gcagttcgaa gcggaaccag atgtagacca gcatcagcgc gatcgcgacc
     2281 agcagggcca ggatgccgct ggtgaacagc tcgcccgaga ccttggcgcc gacggtggcg
     2341 tcattggtga agcgcacctc gcccagcgcc tgctggatgg cgccgcggac ctccgacttg
     2401 accgcgttgg cgttcttgcc gggcggattc tggaagcgga ccatcacgcc ggcgttggcg
     2461 ccttcgccga aggcctggac cccgacgtcg ccgaggtcct gccggctcac ggccgcgcgg
     2521 acggccgcca ggtcgaccgg cttgggatag gtggtgaact ccagcaccgt gccacccttg
     2581 aagtcgatgc cgcaggtcag ccccccgcag ggcggcttga acggcagcag gaccagggcc
     2641 agcgaggcga tcgtcgcgac gatcgacagg gcgccggcat acttggcgaa atcgacgaac
     2701 cggaagttcg tcttcaccgg caggatcttg atcaaaggcc agaaggacat gtcggcgaac
     2761 ctcaagcgat cggcagcgtc ttgggacgcg cgtagcggaa ccacagggcg agcaggagct
     2821 gggtcaccac gaccgccgtg aacaccgagg tgaagacgcc gatcgacagc gtccaggcga
     2881 agccccggac cgggcccgcg ccaaagaaga acatgatcag cgccgccagc agggtcgtca
     2941 ggttggcgtc gatgatcgtc tccatggccc gcgagaagcc cgcgtccatg gcggtgacca
     3001 gtggcctgcc ggcccgcacc tcgtcccgca tgcgttcgta gatcagcacg ttggcgtcga
     3061 ccgccacggc cagggtcagg atcaggccgg cgatgcccgg cagggtcaga gtcgcctggg
     3121 tcgcgctcat cgccgcgatg atcagcaggc cgttgaccag cagggcgatg accgaaatgc
     3181 cgccgaacag accgccgtag gccagcaaca tgaacaccag gatggccgtg aagccgacga
     3241 tggtggagat cttgcccgcc tcgaccgcgt ccgcgcccag ctcggcgccg acggtgcgct
     3301 gctcctcgac gttcagcggg gccggcaggg cgccggcgcg gagcaggatg gccagctcgt
     3361 tggccgattc gaccgtgtaa ctgccggtga tcacgccgct gccgccggtg atcgccgaat
     3421 tgatccgcgg ggcggagatg accttgccgt cgaggatgat ggcgaaaggc ttgccgatgt
     3481 tgcggctggt cacctcggcg aagcggcggg cgccctggct gttgaagcgg aacgacacct
     3541 ggacctcgcc ggtctggggg tcgttgacct gggcggcgtt ggtcagcatg tcgcccgaga
     3601 cgtccacccg gcgacgcacg acataggccg gggcgtagcg gtcggtgctg ggcaggattt
     3661 ccgaacccgg aggcagccgg ccggcctggg cttcctgcag cgagacggtg gtgtcgacca
     3721 tctggaaggt cagcttggcc gtctggccga tcaccgcctt gagcttttcc ggatcgcttt
     3781 cgcccggggc ctgcaggacg atgcggttgg tgccctggcg ggtgatcgcc ggctcacgcg
     3841 tgcccagttc gtcaacccgc cggcggatga tctcgatcga ctggtcgacc gcgccggagg
     3901 cctgttcggc gagggccgtc tccgagaagg ccagtccgat ccgctggtcg ccgtcgcggg
     3961 tgaccttgag gtccggcttg ccggtcttgg tcagctggat aagccgcgac aggatgttgt
     4021 aggcgttggc cacctgggcc tggtc
//
LOCUS       DAORNX010000274         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16644_length_4038_cov_6.624906, whole genome shotgun sequence.
ACCESSION   DAORNX010000274 DAORNX010000000
VERSION     DAORNX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16644_length_4038_cov_6.624906"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLO65_11630"
     CDS             complement(<1..172)
                     /locus_tag="PLO65_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRD28942.1"
                     /translation="MAIKTRFTELFGVEHPIAQGGMQWVGKAELVSAVANAGALGFIT
                     ALTQPTPEDLAKE"
     gene            complement(294..713)
                     /locus_tag="PLO65_11635"
     CDS             complement(294..713)
                     /locus_tag="PLO65_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRD28943.1"
                     /translation="MTRALAVAAALALLAPGSALAVDASAIEGRYTPALQACLDSPDG
                     ASTAGTVQCIGAELEVQDAALNAAWRALVADMTPDQKAGLQKAQRAWIAFRDADCAAR
                     YSPDWGSMSTLDANFCVLRRTVERTLELETFRDDGSQ"
     gene            complement(710..1672)
                     /locus_tag="PLO65_11640"
     CDS             complement(710..1672)
                     /locus_tag="PLO65_11640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRD28944.1"
                     /translation="MVQHYLEFERPIAELEGKIEELSRLSDTAGAGSFDSEIEALRAR
                     VGELRRESYARLDAWQKTQVARHPERPHFVNFVEGLIEEFVELRGDRKFADDQAIIGG
                     LGRFRGQPVVVMGHEKGHDTTTRLKHNFGMARPEGYRKAVRLMDMAEQFNMPVISFVD
                     TAGAYPGLGAEERGQAEAIARCTERCLTLSTPMIATITGEGGSGGAIALAGANRVLIL
                     EHSIYSVISPEGAASILWRDGARARDAADQMKITAQDLIRTGVVDRIIEEPAGGAHSD
                     PEAAIRAVGDAVEEELRNLSNMDAEALRAQRAERFYAIGREGLA"
     gene            1910..2695
                     /locus_tag="PLO65_11645"
     CDS             1910..2695
                     /locus_tag="PLO65_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD28945.1"
                     /translation="MGKLDGRVAIITGAASGIGRAAAQLFAEAGATVVLADVSDKVGE
                     TLELVTGAGGKGVALVGDVATPEVVNKAIEAAEMLGGVDIMFANAGVSGGLPGLFELT
                     PEHWTEVLRINTIGVFLAVQAAARLMVARGKGSIICTASVAGIRSGAGGMPYSASKAG
                     VISIVQTGANQLAGTGVRINAVCPGLTETGMTQPVFDRARERGTEGKIGQLNPLRRAA
                     VPEEIARVALFLASDDASYVNGQFIAVDGGLSSSHPVVPGKMA"
     gene            complement(2970..3776)
                     /locus_tag="PLO65_11650"
     CDS             complement(2970..3776)
                     /locus_tag="PLO65_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD28946.1"
                     /translation="MTTPFFDLRGSHNPHRWHMPLTPPICVGPPEHLFMYGGIGLAAS
                     ISALEQTCGRPVVWATAQYLSYARPPSVVDFDVWISNAGKYTTQARVSAHVGETEVVS
                     VLAALGARPDTRDYQWPRMPDIPPPEDCDPVMLWSDDGDNLNRRIERRAPQGRYDLKN
                     RRGEISEDGRMQMWLRTVEDVPVDAAMLAVFADYVPSGFGHALGVWAGGNSLDNTLRV
                     LKIVPTRWVMVEISISGIHAGVGHGAMNLFAETGELMAVASQTVIVRVRE"
BASE COUNT          595 a         1408 c         1426 g          609 t
ORIGIN      
        1 tttccttggc caggtcttcc ggcgtcggct gggtcagggc ggtgatgaag cccagggccc
       61 ccgcgttggc cacggccgag accagctcgg ccttgccgac ccactgcatg ccgccctggg
      121 cgatggggtg ctcgacgcca aacaactcgg tgaagcgggt cttgatggcc atgggggttc
      181 ctctcccgaa catctgtttg agtcacactg agccatgtca ccccgcgtca ggcaaggggg
      241 tgggggccgg cgaatttcag ggtgtttcgg tgggaattct gggtcgcggc cggctattgg
      301 gagccgtcgt ccctgaacgt ctccagctcg agggttcgtt caaccgtccg gcgcaggacg
      361 cagaagttgg cgtcgagggt cgacatcgat ccccagtcgg ggctatagcg ggcggcgcag
      421 tccgcgtcgc gaaaggcgat ccacgcgcgc tgggccttct gcagtccggc cttctggtcg
      481 ggcgtcatgt cggcgaccag cgcccgccag gcggcgttca gcgcggcgtc ctggacctcg
      541 agttcggcgc cgatgcactg gaccgtgccg gccgtcgagg ctccgtccgg cgagtcgaga
      601 caggcctgca gggcgggcgt gtagcgccct tcgatcgccg atgcgtccac cgccagcgcc
      661 gagcccgggg cgaggagggc cagcgccgcc gcgacggcca gggcgcgcgt cacgccaggc
      721 cctcgcggcc tatcgcgtag aaccgttcgg cccgctgggc gcgcagggcc tcggcgtcca
      781 tgttggacag gttccggagc tcctcctcga ccgcgtcgcc gacggcgcgg atggccgcct
      841 cggggtcgga atgggccccg ccggccggct cctcgatgat ccggtcgacc actccggtcc
      901 tgatcaggtc ctgggcggtg atcttcatct ggtcggcggc gtcccgcgcc cgggccccgt
      961 cgcgccagag gatcgaggcc gcgccctcgg gactgatcac cgaatagatc gaatgctcga
     1021 gaatcagcac ccggttggcg ccggccaggg cgatggcccc gcccgatccg ccctcgccgg
     1081 tgatggtggc gatcatcggc gtcgataggg tcaggcagcg ctcggtgcag cgggcgatgg
     1141 cctcggcctg gccgcgctcc tcggccccca ggcccggata ggcgccggcc gtgtcgacga
     1201 agctgatcac cggcatgttg aactgctcgg ccatgtccat cagccgcacc gccttgcggt
     1261 agccttcggg gcgggccatg ccgaaattgt gcttcaggcg ggtggtggtg tcgtggccct
     1321 tctcgtggcc catcaccacc acgggctgac cgcgaaagcg cccgaggccg ccgatgatcg
     1381 cctggtcgtc ggcgaacttg cggtcgccgc gcaactcgac gaactcctcg atcaggccct
     1441 cgacgaagtt gacgaaatgc ggccgctcgg ggtggcgcgc gacctgggtc ttctgccagg
     1501 cgtccagccg cgcataggac tcgcggcgca gttcgccgac ccgcgcccgc agcgcctcga
     1561 tctcgctgtc gaacgaaccc gccccggccg tgtcggaaag ccgcgacagc tcctcgatct
     1621 tgccttccag ctcggcgatc ggacgctcga actcgagata gtgctggacc atgaatcgcc
     1681 taacgcggcc gcccctgaaa ggtcgcgaaa ctagaggctg gcgggaggag gggcaaggcg
     1741 ggcgttggag cgtagggagt agggagtagg gagtagggag tagcggcagg gggcgcgcgg
     1801 cgccggcggc gggaacaggt cgaactactc cctactccct acgccctatt ccctaatccc
     1861 ctccccccgt gcatgttcgg tcaaacaacc gtttgaagga aacaccacca tgggcaagct
     1921 cgacggccgc gtggccatca tcaccggggc ggccagcggc atcggccgcg cggcggcgca
     1981 gctgttcgcc gaggcgggcg cgacggtggt gctggcggat gtgtccgaca aggtgggcga
     2041 gacgctcgaa ctggtgaccg gcgccggcgg caagggcgtg gccctggtcg gcgacgtcgc
     2101 cacccccgaa gtggtcaaca aggctatcga ggcggccgag atgctcggcg gggtcgacat
     2161 catgttcgcc aacgccggcg tgtccggcgg cctgccgggc ctgttcgaac tgacccccga
     2221 acactggacc gaggtgcttc gcatcaacac catcggcgtg ttcctcgccg tccaggccgc
     2281 cgcccggctg atggtcgcgc gcggcaaggg ctcgatcatc tgcacggcca gcgtggccgg
     2341 catccgctct ggcgccggcg gcatgcccta cagcgcctcc aaggccgggg tgatcagcat
     2401 cgtccagacc ggggccaacc agctcgccgg caccggcgtg cggatcaacg ccgtctgccc
     2461 cggcctgacc gagaccggca tgacccagcc ggtgttcgac cgcgcccgcg agcggggcac
     2521 cgagggcaag atcggccagc tcaatcccct gcgccgcgcc gccgtcccgg aggaaatcgc
     2581 ccgcgtggcc ctgttcctgg cctcggacga cgccagctac gtcaacggcc agttcatcgc
     2641 cgtcgacggc ggcctgtcga gctcgcaccc ggtggtgccg ggaaagatgg cctagctcca
     2701 cactcccatc aggtgtccgt tgccggccga gtgatgactg gccctttggc cttgatggaa
     2761 tgatgcgctc cggacggtcg tccagggcca gaagcgcgag atggaggtcc ggccgttcta
     2821 cggggccgag gacctcgcgg cggcccgcga cgggcagcgg ggcaggctcg gcgtcgcctg
     2881 agacggctgg accggcccct acgccagcgg cgcgatctgc gccggaaggg gccggccatc
     2941 aggcctcagc gaaagtggcc gcggccgccc tactcccgca cccgcacgat caccgtctgg
     3001 ctggccacgg ccatcagctc gccggtctcg gcgaacaggt tcatggcgcc gtggccgacg
     3061 ccggcgtgga tgccgctgat cgaaatctcg accatcaccc agcgggtcgg cacgatcttc
     3121 agcacccgca gggtgttgtc gaggctgttg ccgccggccc agacgcccag ggcgtggccg
     3181 aagcccgacg ggacgtagtc ggcgaacacg gccagcatgg cggcgtcgac gggcacgtcc
     3241 tcgacggtcc gcagccacat ctgcatgcgg ccgtcctcgc tgatctcccc ccggcggttc
     3301 ttcaggtcgt agcgaccctg cggcgcgcgg cgctcgatgc gccggttgag attgtcgccg
     3361 tcatcggacc acagcatcac cgggtcgcaa tcctccggcg gcgggatgtc gggcattctc
     3421 ggccactggt agtcgcgcgt gtccggccgg gcgccgagcg cggccagcac gctgaccacc
     3481 tcggtctcgc cgacatgggc gctgacccgg gcctgggtgg tgtacttgcc ggcgttggag
     3541 atccagacgt cgaaatcgac caccgacggc ggccgggcat aggacaggta ctgggccgtg
     3601 gcccagacca ccggccggcc gcaggtctgc tccagcgccg agatcgaggc ggccaggccg
     3661 atgccgccgt acatgaacag gtgctcgggc ggcccgacgc agatcggcgg cgtcaacggc
     3721 atgtgccagc ggtgcggatt gtgcgacccg cgcaggtcga agaagggggt ggtcatggag
     3781 ggcggtgtag ccgggagcgc ccttcgaagg ctagggcgta gggagtaggg agtagccaga
     3841 gggatgcgcg cggcgccaac ggtgcgagca ggtcgaactc ctccctactc cctactccct
     3901 actccctact ccctacgccc tactccctcg ccgcaaccgc agtgctaagg tccagcgcat
     3961 ggacgccccc gcccgcccga tcatcccgcc cgcgccctat gtgcacccgc aggacctgtc
     4021 gacgccgagg ctggtggt
//
LOCUS       DAORNX010000275         3988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16916_length_3988_cov_9.389525, whole genome shotgun sequence.
ACCESSION   DAORNX010000275 DAORNX010000000
VERSION     DAORNX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16916_length_3988_cov_9.389525"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLO65_11655"
     CDS             complement(<1..247)
                     /locus_tag="PLO65_11655"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRD28947.1"
                     /translation="MITLILVVSLGLSLAMMLVWAFQRAKRNGGWTDVGWTLATGAAG
                     VAYALWPTGAETTARQWLCAGLISLWSLRLGLHLARRV"
     gene            complement(305..381)
                     /locus_tag="PLO65_11660"
     tRNA            complement(305..381)
                     /locus_tag="PLO65_11660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Val,seq:tac)
     gene            complement(482..757)
                     /locus_tag="PLO65_11665"
     CDS             complement(482..757)
                     /locus_tag="PLO65_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRD28948.1"
                     /translation="MTKAELVAAIADGAGLNKTQAAAALQAFVDSVTASLKSGNDVRL
                     VGFGTFKAVNRAAGTARNPRTGETVKRPASKTARFQVGEGLKSTLNG"
     gene            complement(912..3311)
                     /gene="lon"
                     /locus_tag="PLO65_11670"
     CDS             complement(912..3311)
                     /gene="lon"
                     /locus_tag="PLO65_11670"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRD28949.1"
                     /translation="MTDIRTLPVLPLRDIVVFPHMVVPLFVGRDKSVRALEEVMRGDK
                     QILLATQKNSADDDPATDDIYDVGVVATVLQLLKLPDGTVKVLVEGKHRAAVIKFTGR
                     DDFYEAETALIEEDGGEEHEAEALSRAVAEQFENYVKLNKKIPPEALASIPQITEPGK
                     LADSISAHLSVKIGDKQQLLEIFNVVKRLEKVFALMEGEISVLQVEKKIRSRVKRQME
                     KTQREYYLNEQMKAIQRELGDQDDGRDELIELEKRIKKTRLSKEARAKAEAELKKLRN
                     MSPMSAESTVVRNYLDWLLSIPWGKAKPKKIDLARAEAILEEDHYGLEKVKERILEYL
                     AVQARTNALKGPILCLVGPPGVGKTSLGKSIAKATGREFVRLSLGGVRDEAEIRGHRR
                     TYIGSMPGKVIQSMKKAKAVDPFFLLDEIDKMGSDYRGDPSSALLEVLDPAQNSTFAD
                     HYLEVDYDLSQTMFVTTANSLNMPQPLMDRMEIIRIPGYTEDEKVEIAKRHVLPKLIK
                     DHGLAPAEFVVPDEAIRDLIRYYTREAGVRSLERELGNLARKVVRDLAREGVASITID
                     DERLAKYAGVKKYRYGETDAEDQVGIVTGLAWTEFGGDILTIEAIRMSGKGRMSITGN
                     LKDVMKESISAANSYVRSRAPAFGIKPSVFEKTDIHVHVPDGATPKDGPSAGAAMATA
                     MVSVLTGIPIRKDIAMTGEITLRGRVTAIGGLKEKLLAALRSGVKTVLIPQENEKDLA
                     DVPENVKRDVEIIPVSTIDQVLALALTGPLTPIEWTDADESIAATAKADEAVDDAVIT
                     H"
     gene            complement(3512..3946)
                     /locus_tag="PLO65_11675"
     CDS             complement(3512..3946)
                     /locus_tag="PLO65_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD28950.1"
                     /translation="MPQGAALTEAVRARDAEFFAVFFTGCDPARVESMLTPDFEMFHD
                     RGGFIADGAAGFVADYAQACEARKAPDAWRSRRELVPASLRVEPIPGYGAFEEGDHLF
                     YERQGDGPERLAGRAHFVQVWRLAPDGWRLARVFSYSHEAAE"
BASE COUNT          591 a         1366 c         1324 g          707 t
ORIGIN      
        1 cgacccgccg ggccaggtga aggcccagcc gcagggacca gaggctgatc aggccggcgc
       61 acagccactg ccgcgccgtg gtctccgccc cggtcggcca gagggcgtag gcgacgccgg
      121 cggctccggt ggccagggtc cagccgacgt cggtccagcc gccgttccgc ttcgcccgct
      181 ggaaggccca gacgagcatc atggccagcg acaggcccag cgaaacgacg aggatcaggg
      241 tgatcatgag gggggatacg cagcggcccg cgaattggat cgccgctccc cttcccccgg
      301 agcctggtgg gcggcgaggg gttcgaaccc ccgaccctct cggtgtaaac gagatgctct
      361 gaccagctga gctagccgcc cgtcacgcgg gtcatagccg tcccgcgcca tctcgaaaag
      421 caaggaggcg gccgccttgc cggcgaccgc ctcccgcgat gtctcgacct tgaagcggcg
      481 tttagccgtt cagggtgctc ttcaggcctt cgccgacctg gaagcgggcg gtcttggacg
      541 ccgggcgctt cacggtttcg ccggtgcgcg ggttgcgcgc ggtgccggcg gcccgattga
      601 cggccttgaa ggtgccaaag ccgaccaggc ggacgtcgtt gccgctcttg agcgacgcgg
      661 tcaccgagtc gacgaacgcc tgcagcgccg ccgcggcctg cgttttattg agacccgcgc
      721 catcggcgat ggcggcgacc agttcggcct tggtcattga attctcccag ccaagtgttg
      781 tgcggcgcgg cgttcccccc aagggcgagc cgttcgcctg caagggatta tggcggatgg
      841 cgcccctagg tcaaacgaaa ggccgcccgg gaatcacccg ggcggcccac attatcgttg
      901 ataacctagc ttcagtgggt gatcaccgcg tcatcgacgg cctcatcggc cttcgcagtc
      961 gcagcaatcg actcatcggc atccgtccac tcgatcggcg tcagagggcc ggtcagggcc
     1021 agggcgagca cctgatcgat ggtcgacacc gggatgatct ccacgtcgcg cttcacgttc
     1081 tcgggcacgt cggccaggtc cttctcgttc tcctgcggga tcagcaccgt cttcacgccc
     1141 gatcgcaggg ccgccagcag cttctccttc aggccgccga tggccgtgac ccgaccgcgc
     1201 agggtgatct cgccggtcat ggcgatgtcc ttgcggatcg gaatgccggt cagcaccgag
     1261 accatcgccg tggccatggc cgcgccggcc gaggggccgt ccttgggcgt cgcgccgtct
     1321 ggcacgtgca catggatgtc ggtcttctcg aacaccgagg gcttgatgcc gaaggccggc
     1381 gcccgcgagc ggacgtagct gttggcggcc gagatcgact ccttcatcac gtccttgagg
     1441 ttgccggtga tcgacatccg gcccttgccc gacatgcgga tggcctcgat ggtcaggatg
     1501 tcgccgccga actcggtcca ggccaggccg gtgacgatgc cgacctggtc ctccgcgtcc
     1561 gtctcgccat agcggtactt cttgaccccg gcgtacttgg ccaggcgttc gtcatcgatg
     1621 gtgatcgagg cgacgccctc gcgcgccagg tcgcggacga ccttgcgggc caggttgccc
     1681 agttcccgct ccagcgaccg cacgccggct tcccgggtgt agtagcggat caggtcgcgg
     1741 atcgcctcgt cgggcacgac gaactcggcg ggcgcaaggc cgtggtcctt gatcagcttg
     1801 ggcagcacgt ggcgcttggc gatctcgacc ttctcgtcct cggtgtaacc ggggatgcgg
     1861 atgatctcca tccggtccat cagcggctga ggcatgttca ggctgttggc cgtcgtgacg
     1921 aacatcgtct gcgacaggtc gtagtcgacc tcgagatagt gatcggcgaa ggtcgagttc
     1981 tgcgccgggt cgagcacctc cagaagggcc gacgacgggt cgccgcgata gtcgctgccc
     2041 atcttgtcga tctcgtcgag caggaagaac ggatcgaccg ccttggcctt cttcatcgac
     2101 tggatgacct tgccgggcat cgaaccgatg taggtgcggc ggtgaccgcg gatttcggcc
     2161 tcatcgcgca cgccgccgag cgacagccgc acgaactcac ggccggtcgc cttggcgatc
     2221 gacttgccca gcgaggtctt gccgacgccg ggagggccga cgaggcacag gatcggcccc
     2281 ttgagcgcgt tggtccgggc ctggacggcc agatactcga ggatacgttc cttgaccttc
     2341 tccaggccgt agtggtcctc ctcgaggatg gcctcggcgc gggccaggtc gatcttcttc
     2401 ggcttggcct tgccccacgg gatcgacagc agccagtcga ggtagttgcg gaccaccgtg
     2461 ctctcggccg acatcgggct catattgcgc agcttcttca gctccgcttc cgccttggcg
     2521 cgggcctcct tgctgaggcg cgtcttcttg atgcgctttt ccagctcgat gagttcgtcg
     2581 cgaccgtcgt cctggtcgcc cagctcgcgc tggatcgcct tcatctgctc gttgaggtag
     2641 tactcgcgct gggtcttctc catctggcgc ttgacgcgcg agcggatctt cttctcgacc
     2701 tgcaggacgc tgatctcgcc ctccatcagg gcgaacacct tctccagccg cttcacgacg
     2761 ttgaagattt ccagcagttg ctgcttgtcg ccgatcttca ccgacaggtg ggccgagatg
     2821 ctgtcggcca gcttgcccgg ctcggtgatc tgcgggatcg aggccagggc ctccggcgga
     2881 atcttcttgt tcagcttgac gtagttctcg aactgctcgg ccaccgcgcg cgagagggcc
     2941 tcggcctcgt gctcctcgcc gccgtcctcc tcgatcagtg cggtctcggc ctcgtagaag
     3001 tcgtcgcggc cggtgaactt gatcacggcg gcgcggtgct tgccctcgac cagcaccttg
     3061 accgtgccgt ccggcagctt gagcagctgc agcacggtgg cgaccacccc gacgtcatag
     3121 atgtcgtcgg tggcgggatc gtcgtcggcc gagttcttct gggtggccag caggatctgc
     3181 ttgtcgccgc gcatcacctc ttcgagcgcg cgcacggact tgtcgcgccc cacgaacagg
     3241 ggaacgacca tatggggaaa caccacgatg tcccgcagcg gcaacaccgg aagcgtacgg
     3301 atatcggtca taccattctc ctccgcgcgg ccctgcaacg tcggcgccgc gcaggacgcc
     3361 ctacactggg cggcctttgt gaatgatatg tggccgcttc gccgcgccgt tcaagcacgg
     3421 gcggcgccgc gcggcggagg gacgcaggga aaagcacggt tttccacggg atttggcggc
     3481 ccgtcaccgg aggcccgacc ggaaacggcc cctactccgc cgcctcatgg ctgtagctga
     3541 acaccctcgc cagccgccag ccgtccgggg ccagcctcca gacctggacg aagtgggccc
     3601 ttcccgccag cctttcggga ccatcgccct gccgttcgta gaacaggtgg tcgccctcct
     3661 cgaacgcgcc atagccgggg atcggctcga cccgcaggct ggccgggacc agctcgcgcc
     3721 gcgagcgcca ggcgtccggc gcctttcgcg cctcgcaggc ctgcgcatag tcggcgacaa
     3781 aaccggcggc cccgtcggcg atgaagccgc cccggtcgtg gaacatctcg aagtcgggcg
     3841 tcagcatcga ctcgacccgg gccggatcgc agccggtgaa gaacacggcg aagaactcgg
     3901 cgtcccgcgc ccggacggct tcggtcagcg ccgcgccctg cggcaggacc ggtccggcgg
     3961 ggggcgtctg ggcggcggcg gggacggc
//
LOCUS       DAORNX010000276         3986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_16922_length_3986_cov_8.077843, whole genome shotgun sequence.
ACCESSION   DAORNX010000276 DAORNX010000000
VERSION     DAORNX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_3986_cov_8.077843"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1542
                     /locus_tag="PLO65_11680"
     CDS             217..1542
                     /locus_tag="PLO65_11680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRD28951.1"
                     /translation="MSIQARILIVARDDALAGPLAEGLDRLGWRTITARGPYAAIAAL
                     SDLAIEAAIVDVASGGHDALTLARRLKAACAPRRLPVIAIGDPDPQLDSYAFDLTLAP
                     PLHPAQAVLRLESLVRMAVAEEEFELRLETFAERGRRLDLPEPKADPYRVLAVGEPAP
                     QFLALSNALTRSGADVVGAFTAFTAFDYLHERDFDSVVLWAGDNSAEALSIAAGMRRN
                     TRLYHIPALLYLRAESYVTTSEAFHRGVSDVASPETPETETAKRVIELSRSYRRAVSI
                     RKALERARSSGLMDAATGLFTRDLFAGHLVRLAHASRVRNRPMSVCVLRLAERPETTA
                     ARAGGYLDRAIPQIGSMIGRLVRVEDTAARLGPEVFALALPATPQSAGRAAAERIAAV
                     IACTAFEAGDGKPPFTVDFDIGIAQLEPGENAARALERAASRALQREAS"
     gene            1563..2195
                     /locus_tag="PLO65_11685"
     CDS             1563..2195
                     /locus_tag="PLO65_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRD28952.1"
                     /translation="MQIDFIADVVCPWCYLGWRRLQKALALRPDLQATVTWRPYQLDP
                     TLPDEGVDRKAYYAAKFPDPERREAGAKVLNAAAAEDGIRWNLADIPVSPNTNAAHRL
                     IRWAQGEGLQEPVVEGLLAAYFSELKDIGDPVVLADIGAAAGMDRLRLLQAFSEGVDK
                     DTVAREHFQAYQAGVSGVPFMIFDGKLAVSGAEPPERLVKAIDKARELAA"
     gene            2192..2716
                     /locus_tag="PLO65_11690"
     CDS             2192..2716
                     /locus_tag="PLO65_11690"
                     /inference="COORDINATES: protein motif:HMM:NF018951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 family protein"
                     /protein_id="HRD28953.1"
                     /translation="MIDLKDPNVVGPLIGVAVLIVVMGFRFMRANTARPLKLEFMWIM
                     PAYLLAISAFLLWQMPPHGIEWLWLALAFGAGAALGWQRGRMMAIAIDPETHTLSQTT
                     SRAALVFLLALVAVRFGLRSVLTGEASALHLSLAFVTDVFVIFAVGLLGVTRLEMFLR
                     ARRLLTEARAAKAA"
     gene            complement(2718..>3986)
                     /locus_tag="PLO65_11695"
     CDS             complement(2718..>3986)
                     /locus_tag="PLO65_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRD28954.1"
                     /translation="VDEEQHFGVKHKEKLKELRADVHMLTLTATPIPRTLQMALSGIR
                     EMSIIATPPVDRLAVRTYITPWDPVTLREALLREKYRGGQAYFVVPRIKDLTDVERFL
                     REQVPEVKFVVGHGQMSPTQLEEVMSAFYDGQYDVLLSTTIVESGLDIPTANTLVIHR
                     ADMFGLAQLYQLRGRVGRAKARAYAYMTTPAEKQLTLSAEKRLKVLQSLDSLGAGFQL
                     ASHDLDIRGGGNLLGDEQSGHIKEIGVELYQQMLEDAVAELRQRQGAEALADRGWSPL
                     INTGAAVMIPEAYVPDLNVRLALYRRLSEAEQSQDREALAAELIDRFGPIPPEASSLL
                     KVVAIKGLCREANVAKIDVGPKGAVASFRDDHFANPMALVKFIQANQIVWKLRPDQKI
                     VVKGEWDTPAQRLDAAERILTTLAKLAKAG"
BASE COUNT          552 a         1409 c         1362 g          663 t
ORIGIN      
        1 gaaagcacat gggcgaagcg ccgtccgagc ccgctcgagg cgccggtgac gacggcgacg
       61 cgtccggcca ggggtttctc gctcatcatc gtctcctgcg gcgctgtatc gctatgccgt
      121 cccctcgcct ctgctatggt gcgacgttgc gaatcttgac gcgcagcgca tagccggcgg
      181 gggcccggcg gcgtcaaggg cgggagttca gacggcttgt caattcaggc ccgtattttg
      241 atcgtcgcgc gggacgacgc gctggcagga ccgttggccg aggggctcga ccgtctgggc
      301 tggcgcacga tcacggcccg ggggccctac gctgccatcg ccgcgctcag cgacctggcc
      361 attgaggcgg ccatcgtcga cgtcgccagc ggcggccatg acgccctgac cctggcccga
      421 cggctgaagg ccgcctgcgc gccgcggcgg ctgccggtga tcgccatcgg cgatcccgac
      481 ccgcagctgg acagctacgc cttcgacctg accctggctc cgccgctgca tccggcccag
      541 gccgtgctgc gactggagtc gctcgtgcgc atggccgtgg ccgaggaaga gttcgagctg
      601 cggctggaga ccttcgccga gcgcggtcgc cgcctcgacc tgcccgagcc caaggccgat
      661 ccctatcgcg ttctggccgt cggcgagccc gcgccgcagt tcctggccct cagcaacgca
      721 ttgacgcgca gcggcgcgga cgtggtcggc gccttcaccg ccttcaccgc cttcgattat
      781 ctgcacgaac gggacttcga ctcggtggtg ctgtgggccg gtgacaacag cgccgaggcg
      841 ctcagcatcg ccgccgggat gcgccgcaac acgcgcctct atcacattcc cgccctgctc
      901 tacctgcggg ccgagagcta tgtgacgacg tccgaggcct tccatcgcgg ggtatcggac
      961 gtggcctcgc ccgagacgcc ggaaaccgaa accgccaagc gggtgatcga actgtcgcgc
     1021 agctaccggc gcgcggtctc gatccgcaag gcgctcgagc gcgcgcgcag cagcggcctc
     1081 atggacgcgg ccaccggcct gttcacccgc gacctgttcg ccggacatct ggttcggctg
     1141 gcccacgcct cgcgcgtccg caaccgcccg atgagcgtct gtgtgcttcg cctggcagag
     1201 cggcccgaga ccacggcggc ccgcgccggc ggctatcttg atcgggcgat cccgcagatc
     1261 ggctcgatga tcggccgcct cgtacgggtc gaggacacgg ccgcccgtct gggcccggag
     1321 gtcttcgccc tggccctgcc cgccacgccg cagtcggccg gtcgcgcggc ggcggagcgg
     1381 atcgccgcgg tcatcgcctg caccgccttc gaggccggcg acggcaagcc gccgttcacg
     1441 gtcgatttcg atatcggtat cgcccagctt gaaccgggcg agaacgccgc ccgggcgctg
     1501 gagcgggccg cgagccgcgc gctgcagcgc gaagccagct aacctcaacg ggagaccccg
     1561 ccatgcagat cgacttcatc gccgacgtcg tctgcccctg gtgctacctc ggctggcggc
     1621 gactgcagaa ggccctcgcc ctgcgaccgg acctgcaggc gacggtgacc tggcggccct
     1681 accagctgga cccgacgctt ccggacgaag gcgttgaccg caaggcctac tatgccgcca
     1741 agtttcccga cccggagcgc cgcgaggccg gcgcgaaggt gctcaacgcc gcggcggcgg
     1801 aagacggcat ccgctggaac ctggccgata ttcccgtctc gcccaacacc aacgccgccc
     1861 atcggctgat ccgctgggcc cagggcgagg gacttcagga gccggtggtc gagggactcc
     1921 tggcggccta cttcagcgaa ctgaaggaca tcggcgatcc ggtcgtgctg gccgatatcg
     1981 gcgcggcggc gggcatggac cggctgcggc tgctgcaggc cttctccgaa ggtgtcgaca
     2041 aggacacggt cgcccgcgaa cattttcagg cctaccaggc cggggtgtcc ggcgtgccgt
     2101 tcatgatctt cgacggcaag ctggccgttt cgggcgccga gccgcccgag cgcctggtca
     2161 aggccatcga caaggcgcgg gagctggcgg cgtgatcgat ctgaaggacc ccaatgtcgt
     2221 cggcccgctg atcggggtcg cggtgctgat cgtggtgatg ggctttcgtt tcatgcgtgc
     2281 caatacggcc aggccgctga aactcgaatt catgtggatc atgccggcct accttctcgc
     2341 catatccgcc tttctgctct ggcagatgcc gccgcacggg atcgaatggc tgtggctggc
     2401 cctcgccttc ggcgcgggcg cggcgctcgg ctggcagaga ggccgcatga tggccatcgc
     2461 catcgacccg gaaacccata ccctaagcca gaccacctcg cgcgcggcgc tggtcttcct
     2521 gctggctctg gtggccgtgc gattcggcct gcgctcggtc ctgaccggcg aggcctcggc
     2581 cctccatctg tcgctggcgt tcgtcaccga cgtcttcgtg atcttcgcgg tcgggttgct
     2641 cggcgtgacc cggctggaga tgttcctccg ggcgcggcgg ttgctgaccg aggcgcgggc
     2701 ggcgaaggcg gcctgactca gcccgccttc gccagcttcg ccagcgtcgt cagaatccgc
     2761 tcggcggcgt ccagccgctg ggccggcgtg tcccactcgc ccttgacgac aatcttctgg
     2821 tcgggccgca gcttccacac gatctggttg gcctggatga acttcaccag cgccatcggg
     2881 ttggcgaaat ggtcgtcgcg gaagctggcc acggcgccct tggggccgac gtcgatcttg
     2941 gccacgttgg cctcgcggca caggcccttg atggccacga ccttgagcag gctgctggcc
     3001 tccggcggta tgggaccaaa gcggtcgatc agctcggcgg ccagggcctc gcggtcctgc
     3061 gactgctcgg cctccgacag gcggcggtag agggcgaggc gcacgttcag gtccggcaca
     3121 taggcctcgg ggatcatcac cgccgcgccg gtgttgatca gcggcgacca gccgcggtcg
     3181 gccagggcct ccgcgccctg cctctggcgc agctcggcca ccgcgtcctc aagcatctgc
     3241 tggtagagct cgacgccgat ctccttgatg tggccgctct gctcgtcgcc gagcaggttg
     3301 ccgccgccgc ggatgtcgag gtcgtgactg gccagctgga agccggcccc caggctgtcg
     3361 agcgactgca gcaccttgag ccgcttctcg gccgacaggg tcagctgctt ctcggccggc
     3421 gtggtcatgt aggcgtaggc cctcgccttg gcccggccca cccggccgcg cagctggtag
     3481 agctgggcca ggccgaacat gtcggcgcgg tggatgacca gggtgttcgc cgtcggaatg
     3541 tccaggccgc tctcgacgat cgtggtcgac aacagcacgt cgtactgccc gtcgtagaag
     3601 gcgctcatca cctcctcgag ctgggtcggg ctcatctggc cgtggccgac gacgaacttc
     3661 acctccggga cctgttcgcg caggaaccgc tcgacgtcgg tcaggtcctt gatcctcggc
     3721 acgacgaaat aggcctgccc gccgcggtac ttctcccgca gcagggcctc tcgcagggtc
     3781 accggatccc aaggcgtgat gtaggtgcgc accgccaggc ggtcgaccgg cggggtggcg
     3841 atgatgctca tctcgcggat gccgctgagc gccatctgca gggtgcgcgg gatcggcgtg
     3901 gcggtcagcg tcagcatgtg cacgtcggcg cgaagctcct tgagcttctc cttgtgcttg
     3961 accccgaagt gctgctcctc gtcgac
//
LOCUS       DAORNX010000277         3973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17002_length_3973_cov_6.440786, whole genome shotgun sequence.
ACCESSION   DAORNX010000277 DAORNX010000000
VERSION     DAORNX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17002_length_3973_cov_6.440786"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLO65_11700"
     CDS             complement(<1..1171)
                     /locus_tag="PLO65_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD28955.1"
                     /translation="MSELTSLTLKAALDGLKSKAFSATEITKAHIAAVEAARPLNAFV
                     LETPDKALDMAAASDARIARGEARPLDGAPLGIKDLFCTQGVRSTAASKILGNFIPPY
                     ESTVTANLFGDGAVMLGKLNMDEFAMGSSNETSAFGNVINPWKSATGGNRALTPGGSS
                     GGSAAAVAADICLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQA
                     GPIAKTVEDAAILLTSMAGHDPKDSTSLDVETPDWAPFVGQSVKGLRVGVPKEYRVDG
                     MPAEIEKLWQDGIDWLKHAGAEIVEVSLPHTKYALPAYYIVAPAEASSNLARYDGMRY
                     GFRAEGDNLTEIYENTRAQGFGNEVKRRILIGTYVLSAGYYDAYYLKAQKVRRRIA"
     gene            complement(1172..1459)
                     /gene="gatC"
                     /locus_tag="PLO65_11705"
     CDS             complement(1172..1459)
                     /gene="gatC"
                     /locus_tag="PLO65_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRD28956.1"
                     /translation="MAIDAATVRKVARLARIAEPEARLESLASELNGILAWIEQLNEV
                     DTDGVEPMTSAVAMAAPLREDVVTDGGDAAKVLANAPKSVDGFFVVPKVVE"
     gene            1685..2152
                     /gene="ruvX"
                     /locus_tag="PLO65_11710"
     CDS             1685..2152
                     /gene="ruvX"
                     /locus_tag="PLO65_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRD28957.1"
                     /translation="MPVLDLLDLPAALPPYAPIVGLDPGEKTIGVAVSDVTRTVASPL
                     ETIEKVKFTQDAARLFQLMESRGAMGIVIGLPMNMDGTEGARAQSNRALARNLLRLKD
                     IPIAFWDERLSTAAVTRVLIDEHDVNRKRRAEVVDKMAAAWILQGALERLRNG"
     gene            complement(2240..3241)
                     /locus_tag="PLO65_11715"
     CDS             complement(2240..3241)
                     /locus_tag="PLO65_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD28958.1"
                     /translation="MTANRQIVLAQLPGADHLGPQHFRLNVGEAGTPGEGEVLLKILY
                     VSLDAANRAWMQGATYRAAIEAGDVMAGGALAEVVESKTAHLAPGDLVFADTGWQEYA
                     VLPAKRLAKIPRMEPLTHLLSVYGVAGLTAYHGLLECGAPKAGETVVVSAAAGSVGSI
                     VGQIARIRGCRVIGIAGGKAKGRLLVDELGFDAVIDYKAENVRKALREAAPNGVDVYF
                     DNTGGDILEAALFNMNTHGRIACCGAVSQYDGAAPPHGPRGIPGLIVTKRLTMRGFIV
                     SDFADKDAQALKDLQAWVAAGSLKVREDVLEGLESLPAALVGLLAGENVGKRMVKVA"
     gene            3413..>3973
                     /locus_tag="PLO65_11720"
     CDS             3413..>3973
                     /locus_tag="PLO65_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRD28959.1"
                     /translation="MSAILLGVLPVFAMIALGWGLKASRFIPEVSWPPIERITYFVFY
                     PGFLMPAVWKADFGSLSAGPLAIGAVGGMAAVALAVLLARPLIRLPDASYTSVFQGAL
                     RWNTFVFLPLAALVFGKAGAGLAAMIMGALIPAINVICVLVMSRWGEGQGGGWRMAAR
                     GLAQNPVLWGCAVGAVFNLLHVPKLPV"
BASE COUNT          560 a         1347 c         1407 g          659 t
ORIGIN      
        1 cggcgatgcg ccggcggacc ttctgggcct tgaggtagta ggcgtcgtag tagccggcgc
       61 tgagcacata ggtgccgatc aggatgcggc gcttgacctc gttcccgaag ccctgggcgc
      121 gggtgttctc gtaaatctcg gtcaggttgt cgccctcggc ccggaagccg tagcgcatgc
      181 cgtcgtagcg ggcgaggttc gaggacgcct cggccggggc cacgatgtag taggccggca
      241 gagcgtactt cgtatgcggc agcgagacct cgacgatctc ggcgccggcg tgcttcagcc
      301 agtcgatgcc gtcctgccag agcttctcga tctcggccgg catgccgtcg acgcggtatt
      361 ccttcggcac cccgacgcgc aggcccttga ccgactggcc gacgaagggg gcccagtcgg
      421 gggtctcgac gtccaggctg gtggagtcct tcggatcatg gccggccatg ctggtcagca
      481 ggatggccgc gtcctcgacg gtcttggcga tcggcccggc ctggtcgagc gagctggcga
      541 aggcgaccac gccccagcgc gagcagcggc cgtaggtcgg cttgatgccg accgtgccgg
      601 tgaaggccgc cggctggcgg atcgagccgc cggtgtcggt cgccgtggcg cccaggcaga
      661 tatcggccgc gaccgccgcc gccgagccgc ccgacgagcc gccgggggtc agggcgcggt
      721 tgccgccggt ggccgacttc cacgggttga tcacattgcc gaaggcgctg gtctcgttcg
      781 acgagcccat ggcgaactcg tccatgttca gcttgccgag catcacggcg ccgtcgccga
      841 acaggttggc cgtgaccgtc gattcatagg gcgggatgaa gttgccgaga atcttcgagg
      901 cggccgtcga gcggacaccc tgcgtgcaga acaggtcctt gatgcccagc ggcgcgccgt
      961 ccagcggccg tgcctcgccg cgggcgatgc gggcgtcgga ggcggcggcc atgtcgaggg
     1021 ccttgtccgg cgtctccagg acaaaggcgt tcagcggccg cgcggcctcg accgccgcga
     1081 tgtgggcctt ggtgatctcg gtggccgaga aggccttgct tttcaggccg tcgagggcgg
     1141 ccttcagggt gagagaggta agctcggaca tctactcgac caccttcggc acaacgaaga
     1201 agccgtcgac cgacttcggc gcattggcca gcaccttcgc cgcgtcgccg ccgtcggtga
     1261 ccacgtcctc gcgcagcggc gcggccatgg ccacggccga ggtcatcggc tcgaccccgt
     1321 cggtgtcgac ctcgttgagc tgctcgatcc acgcgaggat gccgttcagt tccgaggcca
     1381 gcgactccag tcgcgcctcc ggttcggcga tgcgggcgag ccgggcgacc ttgcggacgg
     1441 tcgcggcgtc gatggccatc gtggcctcct ttgtttcgaa cgtgcgggtt agccgccgga
     1501 agggcctgtt tgcaagaact gtcatcctcg aagggtccca atgcccccac agctgtcatc
     1561 ccggccgcag cgaagcgtag agccgggacc cggattcggc tgaacttcga tctgggtccc
     1621 ggatcgcctg cggcgtccgg gatgaccgta tgaggtgggc ctcgaagaca gtatgagagc
     1681 ggacatgccc gtgctcgacc tgctcgacct gcccgccgcc ctgccaccct acgcgccgat
     1741 cgtgggcctg gatccgggcg agaagaccat cggcgtggcc gtctcggatg tcacccgtac
     1801 cgtcgccagc ccgctggaga ccatcgagaa ggtcaagttt acccaggacg ccgcgcggct
     1861 gttccagctg atggagagcc gcggcgcgat gggcatcgtc atcggcctgc cgatgaacat
     1921 ggacggcacc gagggcgccc gcgcccagtc caaccgggcc ctcgcgcgca acctgctgcg
     1981 gctgaaggac atcccgatcg ctttctggga cgagcggctg tcgaccgccg ccgtgacccg
     2041 ggtgctgatc gacgagcacg acgtgaaccg caagcggcgc gccgaggtgg tcgacaagat
     2101 ggcggcggcc tggatcctcc agggggcgct ggagcggttg cggaacggct gaacggggac
     2161 atgcacttca gtgcgtgtcc cccgccgctg gaagctgccg gggacacgta ccgaaggcgg
     2221 tacatgtccc cacgtcgttc taggccacct tcaccattcg cttgccgaca ttctctcccg
     2281 ccagcaggcc gaccagcgcg gcgggcaggc tttccaggcc ctccagcacg tcctcgcgga
     2341 ccttcagcga gccggcggcg acccaggcct ggaggtcctt gagcgcctgg gcgtccttgt
     2401 cggcgaaatc gctgacgata aaaccgcgca tggtcagccg cttggtgacg atcaggccgg
     2461 ggatgccgcg cgggccgtgc ggcggggcgg cgccgtcgta ctgcgagacc gcgccgcagc
     2521 aggcgatgcg gccgtgggtg ttcatgttga acagcgccgc ttccaggatg tcgccgccgg
     2581 tgttgtcgaa atagacgtcg accccgttcg gggcggcctc gcgcagggcc ttgcggacgt
     2641 tctcggcctt gtagtcgatc accgcgtcga acccgagctc gtcgaccagc agtcggccct
     2701 tggccttgcc gccggcgatg ccgatcaccc ggcagcccct gatcctggcg atctggccga
     2761 cgatcgagcc cactgacccg gcggcggccg agaccaccac cgtctccccg gccttgggcg
     2821 cgccgcactc cagcaggcca tggtaggcgg tcagcccggc gacgccatag acgctgagca
     2881 gatgggtcag cggctccatg cgcgggatct tcgccaaccg ctttgcgggg agcaccgcgt
     2941 actcctgcca gccggtgtcg gcgaacacga gatcgccggg cgccagatgc gcggtttttg
     3001 actcgaccac ctccgccagc gccccgccgg ccatcacgtc gccggcctcg atggccgccc
     3061 gataggtcgc cccctgcatc caggcccggt tggcggcgtc gaggctgacg tagagaatct
     3121 tcagcagaac ctcgccctcg cccggcgtcc cggcctcgcc cacgttcagg cggaagtgct
     3181 gcgggccgag gtggtcggcc ccgggcagct gcgcgagcac gatctggcga ttggcggtca
     3241 tggcggcgtt tccctggact gttgcgggca gtgaacaccg gcccggcgcg gcgggaaagc
     3301 cattgcgtcc ttcgacacgc ccgctatgcg ggctgctcag gatgacgtgc atcggcgcac
     3361 gtcatggtga gcagcgcgtg tcgaaccacg cattcaaggt ccggcttcgt caatgagcgc
     3421 catcctcctc ggggtcctgc ccgtcttcgc catgatcgcc ctgggctggg ggctgaaggc
     3481 ctcgaggttc attcccgagg tcagctggcc gccgatcgag cggatcacct atttcgtctt
     3541 ttacccgggc ttcctgatgc cggcggtgtg gaaggccgac ttcggcagcc tgtcggccgg
     3601 gccgctggcg atcggggcgg tcggcggcat ggccgcggtg gcgctggccg tgctgctggc
     3661 caggcctctg atccgcctgc ccgacgccag ctacaccagc gtgttccagg gcgccctgcg
     3721 gtggaacacc ttcgtcttcc tgccgctggc ggcgctggtc ttcggcaagg cgggcgctgg
     3781 actggcggcg atgatcatgg gggccctgat cccggccatc aacgtcatct gcgtgctggt
     3841 catgagccgc tggggcgagg gccagggcgg cggctggcgc atggccgcgc gcggcctggc
     3901 gcagaacccg gtgctgtggg gctgcgcggt gggggcggtg ttcaacctcc tgcatgttcc
     3961 gaagctgccg gtg
//
LOCUS       DAORNX010000278         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17019_length_3971_cov_7.118999, whole genome shotgun sequence.
ACCESSION   DAORNX010000278 DAORNX010000000
VERSION     DAORNX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17019_length_3971_cov_7.118999"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLO65_11725"
     CDS             complement(<1..215)
                     /locus_tag="PLO65_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28960.1"
                     /translation="MVSSAQRVSNHAPCPGFAASPCGGLAMAINGEPAVLRASGAMWL
                     PAWRALVVADLHFEKGSAYARRGQLLPP"
     gene            complement(280..2814)
                     /locus_tag="PLO65_11730"
     CDS             complement(280..2814)
                     /locus_tag="PLO65_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRD28961.1"
                     /translation="MDGALSPLLPPRFGDWFASRGWAVREHQLAMIDRARAGRDALLI
                     APTGGGKTLAGFLPSLIELSERPPANGPRGIHTLYLSPLKALAVDVERNLLSPITEMG
                     LDIVAESRTGDTGVSRKQRQKVRPPDMLLTTPEQLALLLAWEGARGLFEDLRCVIIDE
                     IHTLWPSKRGDLLALGLSRLQALAPRMRRVGLSATVDDPDLIREWLSSHQQPIAPAEA
                     GAQAMPAGFPTYGLGPRLRGDNRVDLVRGPPGPSPVVDVLIAEGGVPWAGHTAQHAMG
                     QVYEIIKRHRTTLIFVNTRFQAEFAFQELWKLNDDSLPIALHHGSLAAEQRRRVEAAM
                     ARGELRAVVCTSTLDLGIDWGDVDLVIQLAAPKGAARMVQRIGRANHRLDEPSKALFV
                     PANRFEVLECQAAIEAIAENALDGDPPRTGALDVLAQHVMGCACSEPFDLLALYDEVR
                     GAGPYRDLAWEDFEAVVEFVSTGGYALKTYDRFRRIVRNQDGLWTARNAETVRRHRMN
                     VGAIVSPAMLSVRLTGRGGRPGRKLGEMEEGFLEMLSPGDTFIFGGQVWRLHAVTGVD
                     VLVTPAPHVDPKMPSWGSSKFAISTFLAGKVRRMMSDDTSWHAFPDEVREWLEAQRLH
                     SALPRPDEMLVETFPQGKRHFLTCYPFDGRHAHTTLAMLLTRRLDRLGIGPLGFVCND
                     YSMAIWSLRPMDGLDLDALFEEDMLGDDLEAWLDESFLMKRAFKGVALISGLIERRMP
                     GHEKSGRQVTFSTDLIYDVLRRHQPDHLLLRCARSDAATGLVEVGRVAQMLGRIRGHI
                     RHMSLGHVSPFAVPMFMELGRERAPGGASEMMLAEAAEALIAEAMG"
     gene            complement(2820..3485)
                     /locus_tag="PLO65_11735"
     CDS             complement(2820..3485)
                     /locus_tag="PLO65_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HRD28962.1"
                     /translation="MTLSRATTPPLLTAHWRPAVAAIVGVGAGLTAAGLPLLDRHPLL
                     IGWDAGALTYIALLWGLFLTADEKAIRDRAGRQDDGAGIVMLVVMAAIGASLGGIVMA
                     LLNPDPDHRAVTPILVAATLALGWGLMHSVFVPHYAHRHFAAVARNKDAGVGFPGEPP
                     RSYLDFAYMAFTIGATFQVSDNTVGSTRLRNLVTAHALAAYVYNTAILTVGISLLANA
                     VSS"
     gene            3607..>3971
                     /locus_tag="PLO65_11740"
     CDS             3607..>3971
                     /locus_tag="PLO65_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD28963.1"
                     /translation="MRLWWCVPAVLAGFLFAAPVMAAESGRLDNPVAGSPAPTTPADD
                     DLVALRRTAAHGDAEAMYDLAMVYRDGEGTPKDLKKALYWFRKSAARGKAEAMNELGC
                     AYSRGAGVALDYSAALGWFR"
BASE COUNT          603 a         1408 c         1403 g          557 t
ORIGIN      
        1 ggcggcagca gctggccgcg ccgggcgtag gccgagccct tctcgaaatg caggtcggcg
       61 acgaccagcg cccgccaggc cggcaaccac atcgcgccgg acgcgcgcag gacggccggc
      121 tcgccgttga tcgccatggc cagcccgccg cagggggagg cggcgaagcc ggggcacggt
      181 gcgtggttcg acacgcgctg cgcgctgctc accatgacgt acacccttgc acgtcatcct
      241 gagcaggccc gtaggaccgt gtcgaaggac gcgcgatcct catcccatcg cctccgcgat
      301 cagcgcttcg gccgcctcgg ccagcatcat ctcgctcgcc ccgccgggcg cgcgctcgcg
      361 gcccagctcc atgaacatcg gcacggcgaa cggcgagaca tggcccagac tcatgtgacg
      421 gatatgaccc ctgatacgac ccagcatctg cgccacccgg ccgacctcga ccagcccggt
      481 cgccgcgtcc gaccgggcgc agcgcagcag caggtggtcg ggctggtggc gacgcagcac
      541 gtcatagatc aggtcggtgg agaaggtcac ctgccggccc gacttctcgt gccccggcat
      601 gcgtcgctcg atcaggcccg agatcagcgc cacgcccttg aacgcccgct tcatcaggaa
      661 gctctcgtcc agccaggcct cgaggtcgtc gccgagcatg tcctcctcga acagggcgtc
      721 cagatcgagc ccgtccatcg gccgcagcga ccagatggcc atcgaatagt cgttgcagac
      781 gaaccccagc ggcccgatgc ccagccggtc cagtcggcgc gtcagcagca tggccagggt
      841 cgtgtgcgca tgccgcccgt cgaacggata acaggtcagg aagtgacgct tgccctgcgg
      901 aaaggtctcg accagcatct cgtcgggccg gggcagggcg gaatggagcc gctgggcctc
      961 cagccactcg cgcacctcgt cgggaaacgc gtgccagctg gtgtcgtcgc tcatcatccg
     1021 ccgcaccttg ccggccagga aggtcgagat ggcgaacttg ctgctgcccc agctgggcat
     1081 cttcgggtcg acgtgcgggg cgggcgtgac cagcacgtcg acgccggtca ccgcgtgcag
     1141 ccgccagacc tgaccgccga agatgaaggt gtcgccgggc gacagcatct cgaggaagcc
     1201 ctcctccatc tcgcccagct tgcgtccggg ccggccgcca cggccggtca gccgcacgct
     1261 gagcatggcc ggcgagacga tcgccccgac gttcatgcga tggcggcgca cggtctcggc
     1321 gttgcgggcg gtccacaggc cgtcctggtt cctgacgatg cggcggaacc ggtcataggt
     1381 cttcagggcg tagccgccgg tcgagacgaa ctcgacgacc gcctcgaagt cctcccaggc
     1441 caggtcgcga taggggccgg cgccgcgcac ctcgtcatac agggccagca ggtcgaaggg
     1501 ctcggaacag gcgcagccca tgacatgctg cgccagcacg tcgagggccc cggtgcgcgg
     1561 cgggtcgccg tcgagcgcgt tctcggcgat ggcctcgatg gccgcctggc attcgagcac
     1621 ctcgaagcgg ttggccggca cgaacagcgc cttcgacggc tcgtccagcc ggtggttggc
     1681 ccggccgatg cgctggacca tgcgagcggc ccccttcggg gcggccagct ggatgaccag
     1741 gtcgacgtcg ccccagtcga tgcccaggtc cagggtcgag gtgcagacaa cggccctcag
     1801 ttcgccgcgc gccatggccg cctcgacccg ccgccgctgc tcggccgcca ggctgccgtg
     1861 gtgcagcgcg atcggcaggc tgtcgtcgtt cagcttccac agttcctgga aggcgaactc
     1921 ggcctggaag cgggtgttga cgaagatcag cgtggttcga tgccgcttga tgatctcgta
     1981 gacctgcccc atcgcgtgct gcgccgtgtg accggcccag gggacgccgc cctcggcgat
     2041 cagcacgtcg accacggggg aggggccagg cgggccacgg accaggtcaa cccgattgtc
     2101 cccgcgaagg cggggaccca agccgtaggt cgggaaaccc gccggcatgg cttgggctcc
     2161 cgcctccgcg ggagcgatcg gttgttggtg gctggaaagc cactcgcgga tcaggtccgg
     2221 atcgtccacc gtggccgaca gcccgacccg ccgcatgcgc ggcgccagcg cctgcagccg
     2281 gctcagcccc agcgccagca ggtcgccgcg cttggacggc cacagggtgt ggatctcgtc
     2341 gatgatcacg cagcggaggt cctcgaacag cccccgcgca ccctcccagg ccagcagcag
     2401 ggccagctgc tccggcgtgg tcagcagcat gtccggcggc cggaccttct gccgctgctt
     2461 gcgcgagacg ccggtgtcgc cggtgcggga ctcggcgacg atgtccaggc ccatctcggt
     2521 gatcgggctg agcaggttgc gctcgacatc cacggccagg gccttcagcg gggagagata
     2581 cagggtgtgg atgccgcgcg gaccattggc cggcggccgt tcgctgagct cgatcaggct
     2641 gggcaggaag ccggccaggg tcttgccgcc gccggtcggg gcgatcagca gggcgtcgcg
     2701 tccggcccgg gcccggtcga tcatcgccag ctggtgctcg cgcacggccc acccccgact
     2761 ggcgaaccag tcgccgaacc ggggcggcag cagcggggac agggcgccgt ccatggcgat
     2821 caggaactga ccgcgttcgc cagcaggctg atgcccacgg tcaggatggc ggtgttgtag
     2881 acataggcgg ccagggcatg agccgtgacc aggttgcgca gccgcgtgct gccgaccgtg
     2941 ttgtccgaca cctggaaggt cgccccgatg gtgaaggcca tgtaggcgaa atcgagatag
     3001 ctgcgcggcg gctcgcccgg aaagccgacg ccggcgtcct tgttccgcgc gacggccgcg
     3061 aaatggcgat gggcgtagtg cggcacgaac accgagtgca tgagacccca gccgagcgcc
     3121 agggtcgcgg ccacgaggat cggcgtcacc gccctgtggt cggggtcagg gttcagcagg
     3181 gccatgacga tcccgcccag gctggccccg atggccgcca tgaccaccag catgacgatc
     3241 ccggccccgt cgtcctgtcg cccggcccgg tcgcggatgg ccttctcgtc ggctgtcagg
     3301 aacaggcccc agagcagggc gatgtaggtc agggcccccg catcccagcc gatcagcagc
     3361 gggtggcggt caagcagcgg caggcctgcc gccgtcaggc cagccccaac cccgacaatg
     3421 gccgcgaccg ccgggcgcca gtgggcggtc agcagcggcg gcgtcgtggc gcgggacagg
     3481 gtcatcgacg caaccgacca tcccggcggc ccggcgtcaa tgttcccttt ctgtttcggc
     3541 ggtcggcgga cttgacgggc cgcgccgcct cgcgttccac tgccgcgggg atcaaggggc
     3601 tccataatgc gtctctggtg gtgtgttccc gctgttctgg cgggtttcct gttcgccgcg
     3661 ccggtcatgg cggccgagag cgggcggctc gacaacccgg tcgccggttc cccggccccg
     3721 accaccccgg ccgatgacga cctcgtcgcc ctgcgccgga ccgcggccca tggcgacgcc
     3781 gaggcgatgt acgacctggc catggtctac cgcgacggcg aggggacgcc gaaggacctg
     3841 aagaaggccc tctactggtt ccggaagtcg gccgcgcggg gcaaggcgga ggccatgaac
     3901 gagctcggct gcgcctactc caggggcgcc ggcgtggcgc tcgactacag cgcggccctc
     3961 ggctggttcc g
//
LOCUS       DAORNX010000279         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17024_length_3970_cov_6.061047, whole genome shotgun sequence.
ACCESSION   DAORNX010000279 DAORNX010000000
VERSION     DAORNX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_3970_cov_6.061047"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..286
                     /locus_tag="PLO65_11745"
     CDS             17..286
                     /locus_tag="PLO65_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRD28964.1"
                     /translation="MPGRRGGNSLGDGWAPREDDAREGVRGGRLVPLESVIRNIARST
                     PGRLLDAGLEDQGGRPIYRVRWQAANGQRIDFIVDARSGAIIGRH"
     gene            294..>461
                     /locus_tag="PLO65_11750"
     CDS             294..>461
                     /locus_tag="PLO65_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD28965.1"
                     /translation="MRILLVEDDPDLSRQLKLALGDAGYAVDHAADGEEAHYLGEGEP
                     YDAVILDLGLPK"
     assembly_gap    462..569
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <570..975
                     /locus_tag="PLO65_11755"
     CDS             <570..975
                     /locus_tag="PLO65_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD28966.1"
                     /translation="DAGADDYLTKPFHTEELLARLRALLRRSAGHATPNLSCGGLRLD
                     PRSARASVNGEPLRLTSLEYRLLHYMIMHQGRVISRTELVEHLYDQDFDRDSNTIEVF
                     IGRVRKKIGPDRIETVRGLGYRLTPLPGEPTE"
     gene            978..2357
                     /locus_tag="PLO65_11760"
     CDS             978..2357
                     /locus_tag="PLO65_11760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD28967.1"
                     /translation="MPGMGRRSLVGRLVLLAAGWSLAVLIVTGLSLTAFFQQAALSRF
                     DLGLVDLIEGLNAGVSVEDGRVIAPALTDARALRVYSGRYWQIAEPGPNNTIKVIVPS
                     RSLFDRELSIPGDVVKQIAATPAVQVFYGADGPLDQPLRATAMQVKLAGRAAPVILMA
                     AEDRSPVDREARRFAALTAGALVLMGAGILAAVFIQVRVGLQPLFRLRGEVADVRKGK
                     IERIVGQYPSELEPLATELNALVAHNQDVVERQRTHVGNLAHALKTPISVMLTEAQQQ
                     PGQLSEVVSRQAEAMRGHVEHHLRRARAAARSQTHGERTLVEPVIDELAVTLERIFLD
                     KGVEIDWRCPDDLCFRGERQDLQEMAGNIMENAGKWSKGRVRVAAVALSPERLAITVE
                     DDGPGLPADRREEVLKRGARLDESAPGSGLGLAIVDELARAYGGALILGDSSMGGLKV
                     ELSLPRAET"
     gene            2415..3773
                     /locus_tag="PLO65_11765"
     CDS             2415..3773
                     /locus_tag="PLO65_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28968.1"
                     /translation="MNLVAGLMGELSPQKIEMLRTLVSASPDELVRTLEHAISADGVT
                     GPLAAIGAMVEAEAAERALRFTVLAPVAPLFGSSSDGRPAYPRSALKLLWTALDEEAP
                     DLMRAAADACHYLDPDEASPEVFDQLCELAAEGLATGASDSYAAAAAACDAVRPGGAA
                     EIALCLRMAPIVRPILQRLGDWVQRMTDERKATARLAYRDATALAEGAGPLLFEMLAA
                     HLRQPAMILRVISAVMDHPGERYMAGSELARFGERALGEVEGQLDRLRGMKPGSGVAA
                     GREAGAAVQRALDAVAELEQSVQLSREGPWGQRLTKLKQSLAAAVEARLREIDEAAAH
                     ALPVQKLRYSAKLIRTAPKLSDPPDEPAINWAMGLLSFADAVRPCAPDGGFGALRTKV
                     LETLGKRIDQYVEDLLEQLRAGELDDAERAREFLGVAANLLALARDDSSAAIVRRRAA
                     AA"
BASE COUNT          567 a         1352 c         1389 g          554 t
ORIGIN      
        1 gggcggagac cgtccgatgc ccggccggcg tggcggcaat tccctcggcg acggctgggc
       61 gccccgcgag gacgacgccc gcgagggcgt gcgcggcggc cggctggtgc cgcttgaaag
      121 cgtgatccgc aacatcgccc gctcgactcc ggggcggctg ctcgacgccg gtctcgagga
      181 ccagggcggg cggccgatct accgcgtgcg ctggcaggcg gccaacggac aacgaatcga
      241 cttcatcgtc gacgcccgct cgggcgcgat catcggcaga cattgagggc cccatgcgca
      301 tcctgctcgt cgaggatgat cccgacctgt cgcggcagct gaagctggcc cttggcgacg
      361 ccggctacgc cgtggatcac gccgccgacg gcgaggaggc ccattatctg ggcgagggcg
      421 agccctatga cgcggtgatc ctcgacctgg gcctgcccaa gnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gacgccgggg ccgacgacta cctgaccaag
      601 cccttccaca ccgaggaact gctggcgcgg ctgcgggcgc tgctgcggcg gtcggccggc
      661 catgcgacgc ccaacctgag ctgcggcggc ctgcgcctcg acccccgttc ggcccgggcc
      721 agcgtcaacg gcgagcccct ccggctgacg tcgctggaat accgcctgct gcactacatg
      781 atcatgcacc agggccgggt gatcagccgg accgagctgg tcgagcacct gtacgaccag
      841 gacttcgacc gcgactcgaa caccatcgag gtgttcatcg gtcgggtgcg caagaagatc
      901 gggcccgatc gcatcgagac cgtccgggga ctgggctacc ggctgacgcc gctgcccgga
      961 gagcccaccg agtgaagctg cccggcatgg gccgccgttc gctggtcgga cggctggtgt
     1021 tgctggccgc cggctggagc ctggccgtcc tgatcgtgac cggcctgtcc ctgaccgcct
     1081 tcttccagca ggccgccctg agccggttcg acctggggct ggtcgacctg atcgaggggc
     1141 tcaacgccgg cgtcagcgtc gaggacggcc gggtgatcgc cccggccctg accgacgccc
     1201 gtgcgctgcg ggtctattcc ggccgctact ggcagatcgc cgagcccgga ccgaacaaca
     1261 ccatcaaggt catcgtgccg tcgcgatccc tgttcgaccg cgagctgagc attcccgggg
     1321 atgtcgtgaa gcagatcgcc gcgacccccg cggtgcaggt gttctacggc gccgatggcc
     1381 cgctcgatca gccgctgcgg gccaccgcca tgcaggtcaa gctggccggg cgggcggcgc
     1441 ccgtgatcct gatggccgcc gaggaccgat cgccggtcga ccgggaggcc cggcggttcg
     1501 ccgccctgac cgcgggggcg ctggtgctca tgggcgccgg catcctggcg gccgtgttca
     1561 tccaggtgcg ggtcggcctg cagccgctgt tccggctgcg cggcgaggtc gccgatgtcc
     1621 gcaagggcaa gatcgaacgc atcgtcggcc agtacccgtc cgagctcgag ccgctggcca
     1681 ccgagctgaa cgccctggtg gcgcacaacc aggatgtcgt cgaacggcag cggacccacg
     1741 tcggcaacct ggcccatgcc ctgaagacgc caatctcggt catgctgacc gaggcgcagc
     1801 agcagccggg tcagctatcc gaggtcgtct cgcggcaggc cgaggccatg cgggggcacg
     1861 tcgagcacca tctgcgccgg gcgcgggccg cggcccggtc ccagacccac ggcgaacgga
     1921 ccctggtcga gcccgtgatc gacgaactgg ccgtgaccct cgaacggatc ttcctcgaca
     1981 agggcgtcga gatcgactgg cgctgtccgg acgacctgtg cttccgcggc gagcggcagg
     2041 acctgcagga gatggccggc aacatcatgg aaaacgccgg caagtggtcg aaggggcggg
     2101 tccgggtcgc cgccgtcgcg ctgtcacccg agcggctggc gatcaccgtg gaggacgacg
     2161 gccccggcct gccagccgac cgtcgggagg aggtgctcaa gcgcggggcg cgtctggacg
     2221 agagcgcgcc cggttccggt ctcggtctgg cgatcgtcga cgaactggcc cgcgcctatg
     2281 gcggcgccct gattctcggc gattcgagca tgggcggatt gaaggtcgag ctgtcgctgc
     2341 cgcgcgccga aacctgaaac aaccatttcg ggaactgtta acccaaatca gtgacggtaa
     2401 gcccgggttc ttgaatgaac ctggtggcag ggctgatggg cgaactctct ccccagaaga
     2461 tcgagatgct caggacgctg gtttcggcct cgccggacga gcttgtccgc accctggagc
     2521 atgcgatcag cgccgacggc gtaaccggtc ccctggccgc catcggcgcc atggtcgagg
     2581 ccgaagccgc cgaacgggcg ctgcggttca ccgtgcttgc cccggtcgcc ccgctgttcg
     2641 gatcgtcgtc cgatggacgc ccggcctatc cccgatcggc gctgaagctc ctctggaccg
     2701 ccctcgacga ggaagcgccc gacctcatgc gggccgcggc ggacgcctgc cactacctgg
     2761 atcccgacga ggcgtcgccc gaggtcttcg accagctgtg cgaactggcc gccgagggac
     2821 tcgccaccgg cgcctcggat tcctacgccg ccgccgccgc cgcctgtgac gccgtccggc
     2881 ccgggggcgc ggccgagatc gcgctctgcc tgcggatggc gccgatcgtg cgacccatcc
     2941 tccagcgcct cggcgactgg gttcagcgga tgaccgatga acgcaaggcg accgcgcggc
     3001 tggcctaccg ggacgcgact gccctggccg agggcgccgg gccgctgctg ttcgagatgc
     3061 tggccgccca cctcaggcag ccggcgatga tcctgagggt catttcggcg gtgatggacc
     3121 atcccggcga gcgctacatg gccggctctg aactggcccg gttcggcgag cgcgcgcttg
     3181 gcgaagtcga aggccagctt gaccgcctgc ggggcatgaa gcccgggtcg ggcgtggcgg
     3241 ccggccggga ggccggggcg gccgtgcagc gggccctcga tgccgtggcg gagctggagc
     3301 aatcggtcca gctgtcgcgc gagggaccgt ggggacagcg gctgacgaag ctgaagcagt
     3361 cccttgccgc cgccgtcgag gcgcggctga gggagatcga cgaggcggca gcgcacgccc
     3421 tgccggttca gaagctgcgc tacagcgcga agctgattcg caccgcgccg aagctcagcg
     3481 atccgccaga cgagccggcg atcaactggg ccatgggcct gctgtcgttc gccgacgccg
     3541 tgcgcccctg cgcgcccgat ggcggcttcg gcgccttgcg gaccaaggtg ctggaaaccc
     3601 ttggaaaacg catcgaccaa tatgtcgagg acctgcttga gcagttgcgg gccggcgaac
     3661 tcgatgacgc cgagcgcgcc cgggaattcc tcggtgtggc cgccaatctc ctggccctgg
     3721 cgcgggacga cagcagcgcc gccatcgtgc gccggagggc tgcggcggcg taacgcggcg
     3781 gcgccttgat ccaaatcctc gcgcggcggc gaatgatccg ctaacacgcc gccatgttcg
     3841 cattctggat cgccgcagcg gccctttcag cggcggccgg cgggttgatg ctgcgcggcg
     3901 cgcggctggc cctggcccgc gcggcgggcg agggcgccgc gttgccgagg gcgacggcga
     3961 tattccgcag
//
LOCUS       DAORNX010000280         3947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17160_length_3947_cov_8.145427, whole genome shotgun sequence.
ACCESSION   DAORNX010000280 DAORNX010000000
VERSION     DAORNX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17160_length_3947_cov_8.145427"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLO65_11770"
     CDS             complement(<1..133)
                     /locus_tag="PLO65_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HRD28969.1"
                     /translation="MRRALLTALCGALIATPALAGPVSLKADVADADGRVTLGDLFDG
                     "
     gene            complement(135..923)
                     /gene="flgG"
                     /locus_tag="PLO65_11775"
     CDS             complement(135..923)
                     /gene="flgG"
                     /locus_tag="PLO65_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRD28970.1"
                     /translation="MQALRTAATGMAAQQLNVEVISNNIANMNTVGFKRQRAEFQDLL
                     YQSIERAGAQSSDQGTVVPTGVQIGGGVKAGSVYRITGQGSMTNTTNTYDLGIQGRGY
                     LQVLLPSGETAYTRAGNLSLNDQGQLVTEDGYQIQPSITIPQDAVDVTISKTGQVQVT
                     KSGAAAPEVAGQIELATFVNEAGLEAMGDNLYMESGASGPATTGAPGSTGFGQLLQGY
                     TEASNVDAVSEITALIVAQRAYEMNSKVISTADEMLQAANQVKS"
     gene            complement(938..1672)
                     /gene="flgF"
                     /locus_tag="PLO65_11780"
     CDS             complement(938..1672)
                     /gene="flgF"
                     /locus_tag="PLO65_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRD28971.1"
                     /translation="MDNAMYVALSRQMTLRRELDIVANNIANVDTTGFKVEGTMVKTD
                     PATGAQMVDGPASLKFVIDDGVTRDFTQGALRQTNGPLDVAIEGQGFFKVQTAAGERY
                     TRDGRFTLSPEGKVTTQAGDPVVGESGEITVNPELGPISIAADGTVSQGAERIGKLDV
                     VTFDDLSGLSKTGDNLFRNTSNLQPQPSTAARVRQGMLEGSNVNSILQVTRLIEISRA
                     YESMARTIENSAELSRRSVERLGRVA"
     gene            1887..2498
                     /locus_tag="PLO65_11785"
     CDS             1887..2498
                     /locus_tag="PLO65_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRD28972.1"
                     /translation="MAKDKVKEAEAAPEGEGAVEGEGAASGKKKPPLKLLIIGGVAAV
                     LVLGGGGAGAFIFLKPKPEAAAGEHGAKPEKKPKKKPKAEHGGGAAEAGAPTVKEGPE
                     GVVFYTLPDIVVNMQTADGRPTFLKLKLTFELPDEEAVEVLNPNMPRLQDMFQTFLRE
                     LRPEDLQGSQGSYQLRMEILRRVNLVVAPTKVNAVLIEEMLIN"
     gene            2525..3646
                     /gene="fliM"
                     /locus_tag="PLO65_11790"
     CDS             2525..3646
                     /gene="fliM"
                     /locus_tag="PLO65_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRD28973.1"
                     /translation="MADETEDQAAMAQWAQENAPPATDDATPAGGDDWGAPAHDDTLI
                     GSERILNQDEIDSLLGFDLSDDDAAERTGIRAIINSALVSYERLPMLEIVFDRLVRLM
                     TTSLRNFTSDNVEVSLDNISSIRFGDYLNSIPLPAILSVFRAEELDNYGLLTVDSNLI
                     YSIVDVLLGGRRGTAAMRIEGRPYTTIERVLVQRMVEVVLADAKAAFEPLTPVNFVLD
                     RLETNPRFAAIARPANAAILVKLRIDMEDRGGRIELLLPYATLEPIRKMLLQQFMGEK
                     FGRDNIWEGHLATELWTTQMEVRAVLDEQQVGLSKVLDLKVGDTFMLNATADSLVELR
                     AGAIPLTRGRMGRRNHHIAVRCEAPLTPAAKQAVQKRLT"
     gene            3643..>3947
                     /locus_tag="PLO65_11795"
     CDS             3643..>3947
                     /locus_tag="PLO65_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HRD28974.1"
                     /translation="MSVAAIIMNLVLSGLLLAALAFGWRLERRLRALKDSHDSFARAV
                     ADLDVAAARAEQGLADLRAATDEAAETLSDRIEAARALSARLERGVSASSIPAPQTEL
                     "
BASE COUNT          623 a         1326 c         1358 g          640 t
ORIGIN      
        1 ccccgtcgaa caggtcgccc agggttaccc ggccgtcggc gtcggcgacg tcggccttca
       61 agctgaccgg cccggccagc gccggcgtgg cgatcagcgc cccgcacagg gcggtcagga
      121 gggcgcggcg catcctacga cttcacctgg ttggccgcct gcagcatctc gtcggcggtg
      181 gagatgacct tggagttcat ctcgtaggcc cgctgggcga cgattagggc ggtgatttcg
      241 ctgacggcgt caacgttcga ggcttcggta tagccctgca gaagctggcc gaagccggtc
      301 gagccgggcg cgccggtggt cgccgggccg gaggccccgc tctccatgta gaggttatcg
      361 cccatggcct cgaggccggc ctcgttgacg aaggtggcca gctcgatctg cccggccact
      421 tccggcgcgg ccgcgccgga cttggtcacc tggacctgcc cggtcttgga gatggtcacg
      481 tcgacggcgt cctgcgggat cgtgatcgac ggctggatct ggtagccgtc ctcggtcacc
      541 agttggccct ggtcgttgag cgacagattg ccggcgcggg tgtaggcggt ctcgcccgac
      601 ggcagcagca cctgcagata gccccgtccc tggatgccca ggtcgtaggt gttggtggtg
      661 ttggtcatcg agccctggcc ggtgatgcgg tagacgctgc cggccttgac cccgccgccg
      721 atctgcaccc cggtcgggac caccgtgccc tggtcggacg actgcgcgcc ggcccgctcg
      781 atcgactggt agagcaggtc ctggaattcc gcccgctgac gcttgaagcc aacggtgttc
      841 atgttggcga tgttgttcga gatgacctcg acgttcagct gctgggcggc catgccggtg
      901 gcggcggtgc gtagagcctg catcaggcgt tactccctca agcgacgcgc cccagccgct
      961 cgacggagcg gcgggacagt tcggcggaat tctcgatggt ccgggccatg ctctcgtagg
     1021 cgcggctgat ttcgatcagg cgggtgacct gcaggatcga attgacgttg ctgccctcca
     1081 gcatgccctg gcggacccgc gcggcggtcg acggctgggg ctgcaggttg gaggtgttgc
     1141 gaaacaggtt gtcgccggtc ttcgacaggc ccgacaggtc gtcgaaggtg accacgtcga
     1201 gcttgccgat gcgctcggcc ccctggctga ccgtgccgtc ggcggcgatg ctgatcgggc
     1261 ccagttccgg attgacggtg atctcgccgc tttcgccgac caccggatcg ccggcctggg
     1321 tcgtgacctt gccttcgggg ctgagggtga accggccgtc gcgggtgtag cgctcgccgg
     1381 ccgctgtctg gaccttgaag aagccctggc cctcaatggc cacgtccagc gggccgttgg
     1441 tctggcgcag cgcgccctgg gtgaagtcgc gggtcacgcc gtcgtcgatg acgaatttca
     1501 ggctggccgg gccgtcgacc atctgcgccc cggtggcggg gtcggtcttg accatcgtgc
     1561 cctcgacctt gaagccggtc gtgtcgacgt tggcgatgtt gttggcgacg atgtccagct
     1621 cgcggcgcag ggtcatctgg cgcgacaggg cgacgtacat cgcgttgtcc aagacgggcc
     1681 tccccgggcg aaatcgtcgc cgattgcgac ccttgccgcc ctgcaacctt cgggccagtc
     1741 gaaaaggcca atgatttcag aagggagagt ggttaacgcc ggccacccgg cggttcttgc
     1801 cgggcgtatt cgaccgccct tcaaaaccga tccttaacca acgtgaacca ttgaggagga
     1861 ggcagggatt ctaaggagcc gcgcgcgtgg ccaaggacaa ggtcaaggaa gccgaggccg
     1921 cccccgaagg ggagggcgcg gttgagggcg agggcgcggc gtccggcaag aagaagccgc
     1981 cgctgaagct gctgatcatc ggcggcgtcg ccgcggtgct ggtcctcggc ggcggcgggg
     2041 cgggggcctt catcttcctc aagcccaagc ccgaggcggc cgccggggaa catggcgcca
     2101 aaccggagaa gaagcccaag aaaaagccca aggccgagca tggcgggggc gccgccgagg
     2161 ccggcgcgcc gaccgtcaag gagggccccg aaggcgtggt gttctacacc ctgcccgaca
     2221 tcgtggtgaa catgcagacg gccgacggcc gcccgacctt cctcaagctc aagctgacct
     2281 tcgagctgcc ggacgaggag gcggtcgagg tgctcaatcc gaacatgccc aggctgcagg
     2341 acatgttcca gaccttcctg cgcgagcttc gcccggaaga ccttcagggc agccagggca
     2401 gctaccagct gcgcatggag atcctccgcc gggtgaacct cgtggtcgcc cccaccaagg
     2461 tcaacgccgt cctgatcgag gagatgctga tcaactaggc tccggcccgg ttgacgcgta
     2521 acgcatggct gacgagaccg aagatcaggc cgcgatggcc cagtgggccc aggagaacgc
     2581 gccgccggcg accgacgacg ccacgccggc cggcggcgac gactggggtg cgccggctca
     2641 cgacgacacc ctgatcggct cagagcggat cctgaaccag gacgagatcg acagcctgct
     2701 gggcttcgat ctcagcgatg acgacgccgc cgaacgcacg ggcatccggg cgatcatcaa
     2761 ctcggccctg gtgtcctacg agcgtctgcc gatgctcgag atcgtcttcg accggctggt
     2821 ccggctgatg accacgtccc tgcgcaactt caccagcgac aacgtcgagg tcagcctcga
     2881 caacatcagc tcgatccgct ttggcgacta tctcaactcg atcccgctgc cggcgatcct
     2941 gtcggtgttc cgcgccgagg agctggacaa ctacggcctg ctgactgtcg attccaacct
     3001 gatctactcg atcgtcgacg tgctgctggg cggccgccgg ggcactgcgg cgatgcgcat
     3061 cgagggccgg ccctacacca ccatcgagcg ggtgctggtg cagcgcatgg tcgaggtcgt
     3121 gctggctgac gccaaggccg ccttcgagcc gctgactccg gtcaacttcg tgctcgaccg
     3181 gctggagacc aatccgcgct tcgcggccat cgcccggccg gccaacgccg ccatcctggt
     3241 caagctgcgc atcgacatgg aagaccgcgg cggccgcatc gagctgctgc tgccttatgc
     3301 gaccctcgag cccatccgca aaatgctgct gcagcagttc atgggcgaga agttcggccg
     3361 cgacaacatc tgggaaggcc acctggccac cgagctgtgg accacccaga tggaggtccg
     3421 cgccgtgctc gacgagcagc aggtcgggct gtcgaaggtg ctcgacctca aggtcggcga
     3481 caccttcatg ctcaacgcca ccgccgacag cctggtcgaa ctgcgggccg gcgccattcc
     3541 cctgacgcgc ggccgcatgg gccgtcgcaa ccaccacatc gccgtgcgct gcgaggcgcc
     3601 gttgacgccg gccgccaagc aggccgtgca gaagagactc acatgagcgt cgccgccatc
     3661 atcatgaacc tggtcctgtc cggtctgctg ctggccgccc tggccttcgg ctggcggctg
     3721 gagcgccggc tcagggcgct caaggacagc catgacagct tcgccagggc ggtggccgac
     3781 ctcgacgtcg cggccgcccg ggcagagcag ggcctggccg acctgcgcgc ggcgaccgac
     3841 gaggcggccg agaccctgtc cgaccggatc gaggcggcca gggccctgtc ggcccggctc
     3901 gagcgcggcg tttccgcttc ctcaatcccg gcgccgcaga ctgagct
//
LOCUS       DAORNX010000281         3947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17161_length_3947_cov_5.558325, whole genome shotgun sequence.
ACCESSION   DAORNX010000281 DAORNX010000000
VERSION     DAORNX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17161_length_3947_cov_5.558325"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>210
                     /locus_tag="PLO65_11800"
     CDS             <1..>210
                     /locus_tag="PLO65_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRD28975.1"
                     /translation="ALSLAGAPGIRIVNRTLDRAEALASDVGAGVQAMTGQGALADAN
                     LIVNATSLGLGGGPGPEVDLAAAAV"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <221..436
                     /locus_tag="PLO65_11805"
     CDS             <221..436
                     /locus_tag="PLO65_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRD28976.1"
                     /translation="APGSAVVLDMVYKPLRTEFLRRAAALGLRTVDGLEMLIRQAVPS
                     FEAFFGQSPPADVDVRALALRAVGDPA"
     gene            433..1053
                     /gene="coaE"
                     /locus_tag="PLO65_11810"
     CDS             433..1053
                     /gene="coaE"
                     /locus_tag="PLO65_11810"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289001.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRD28977.1"
                     /translation="MITVGLTGSIGMGKSTTAAMFAAEGVPVYDADAEVHALYARGGA
                     AVAPLEAAFPGVVVDGAVDRTLLSQRVVGKPDELRRLEAIVHPLVGESRVGFFQKAVA
                     DGADIVVLDIPLLFETGGEKRMDAVVVVSAPADIQRQRVLARPGMDEAKLDAILARQT
                     ADAEKRARAHFVIDTSKGLDDARRQVRDVLTRLRAGPLDAIHEPGQ"
     gene            1061..1762
                     /gene="dnaQ"
                     /locus_tag="PLO65_11815"
     CDS             1061..1762
                     /gene="dnaQ"
                     /locus_tag="PLO65_11815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRD28978.1"
                     /translation="MARHIVLDTETTGLDHRTGHRLIEIACVEVVDYMPTGKSFHEYV
                     HPDRDIDPDAERVHGISLASLHGKPRFPEIVERFLEFVGEDPIVAHNAPFDRGFVNAE
                     LSRCGLAPTPDHQWIDTLALAKKRFPGMYNSLDALCKRMKISLAARDKHGALIDAELL
                     AAVYLELQGGKERALDLHAAPAIRTIGPSTLAAPYGARPRPLAPRSTEDERAAHALFV
                     HGTLKEKAVWLKFGL"
     gene            complement(1766..2263)
                     /gene="secB"
                     /locus_tag="PLO65_11820"
     CDS             complement(1766..2263)
                     /gene="secB"
                     /locus_tag="PLO65_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRD28979.1"
                     /translation="MSDTSTTPPAENGQDISQPGFRILAQFVRDLSFENPLAPESLRS
                     DSQGLQPQIDIGVEMSARGRPDGLFEVDLKLSAQAKRGPSVAFHVELLYGGLFQVTGV
                     PDSEIEAVLMIECPRYLFPFARRLISDVTAEGGFPPFQLDPIDFAGVYAARKAQGESG
                     FTGAA"
     gene            2401..3048
                     /gene="timA"
                     /locus_tag="PLO65_11825"
     CDS             2401..3048
                     /gene="timA"
                     /locus_tag="PLO65_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-related membrane protein TimA"
                     /protein_id="HRD28980.1"
                     /translation="MPINDVSHPGPQVRFLELLIFAFFAAVVLYQLYAVLGRRIGRQP
                     EDLPAAGPAADGERPAVTDAPEAERVDVSGLAAVRARDPSFDVAHFLSGARQAYEMIV
                     IAFAAGDHATLKNLLAPAVAESFEAAIATREAEGRTERVEFLQPPRADLERIEVDGGV
                     ARAVVRFLSEFRSRTKGPEGEAVDDRRTAELWTFERNLNSRDPNWTLVHVDAAEA"
     gene            3213..>3947
                     /locus_tag="PLO65_11830"
     CDS             3213..>3947
                     /locus_tag="PLO65_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRD28981.1"
                     /translation="MPGWDAEDHAAALAAFRATCNVARDVAASAVCRRARALGPLDDA
                     GARRFLEANFRPEPLAESGLLTAYFAPVYEASPVRRGAFTAPVRARPADLVITDLSEI
                     DAGAPSGQTLVGRLEDGRMEPYPDRATIERRGVGRVLAWMRPEDLFFLQIQGSGVLTF
                     PDGSRRRAAFAAHNGHPFAGIARPMREKGLLRDSDTSGDAIRAWLAAHRGREADAIMR
                     LNPRYVFFTLTDDDGRDPAGAAGVPLP"
BASE COUNT          555 a         1452 c         1340 g          590 t
ORIGIN      
        1 ggcgctgtcc ctcgccggcg cgccggggat ccgtatcgtc aaccgcaccc tcgaccgcgc
       61 cgaggccctg gccagcgatg tcggcgctgg cgtccaggcc atgaccgggc agggcgcgct
      121 cgccgacgcc aacctgattg tcaacgccac ctcgctgggc ctgggcggcg ggccgggtcc
      181 cgaggtcgac ctggccgccg ccgcggttga nnnnnnnnnn gcccccggat cggccgtggt
      241 gctcgacatg gtctacaagc cgctgcgcac cgaattcctg cggcgtgcgg ccgctctggg
      301 tctgcgcacc gtcgatgggc tcgagatgtt gatccgccag gcggttccga gcttcgaggc
      361 cttcttcggc cagtcgccgc ccgcggacgt cgatgtccgc gccctggccc tccgggctgt
      421 aggagacccc gcatgatcac cgtcggcctg accggctcca tcggcatggg caaatcgacg
      481 accgcggcga tgttcgcggc cgagggcgtg ccggtctatg acgccgatgc cgaggtccac
      541 gccctctatg cccgcggcgg ggcggccgtg gcgccgctgg aggccgcctt ccccggcgtg
      601 gtcgttgacg gcgcggtcga ccggaccctg ctcagccagc gtgtcgtggg caaacccgac
      661 gagctcaggc ggctggaggc cattgtccac cctctggtcg gtgaaagccg cgtcggcttc
      721 ttccagaagg ccgtggccga cggcgccgac atcgtcgtcc tcgacatccc gctgttgttc
      781 gagaccgggg gcgagaaacg catggacgcc gtcgtggtgg tctcggcccc ggccgacatc
      841 cagcgccaga gggtgcttgc caggcccggc atggacgagg ccaagctcga cgccatcctc
      901 gcccgccaga cggccgacgc cgagaaacgc gcccgcgccc acttcgtcat cgacaccagc
      961 aaggggctcg atgacgcccg ccggcaggtc cgcgacgtgc tgacgcggct gcgcgccgga
     1021 ccgctggacg ccatccacga accgggccaa tgagaaggcc atggcgcgtc acatcgtcct
     1081 cgacaccgag accacgggcc tcgaccaccg caccggccac cggctgatcg aaatcgcctg
     1141 tgtcgaggtg gtcgactaca tgccgaccgg caagtcgttc cacgagtatg tccatccgga
     1201 ccgcgacatc gatccggacg ccgagcgggt gcacggcatc agcctggcct cgctgcacgg
     1261 caagccgcgc ttccccgaga tcgtcgaacg gttcctggaa ttcgtcggcg aggatccgat
     1321 cgtggcgcac aacgccccgt tcgaccgggg cttcgtcaac gccgagctgt cgcgctgcgg
     1381 cctggcgccg acgcccgacc accagtggat cgacaccctg gccctggcca agaagcgctt
     1441 tcccgggatg tacaactccc tggacgcgct ctgtaagcgg atgaagatct cgctggccgc
     1501 gcgcgacaag cacggggccc tgatcgacgc cgagctgctg gccgcggtct atctcgagct
     1561 gcagggcggc aaggagcggg cgctcgacct gcatgccgct cccgccatcc gcaccatcgg
     1621 cccctcgacc ctggccgcgc cctatggcgc gcgcccccgg ccgctcgcgc cccgctcaac
     1681 ggaagacgag cgggcggcgc acgcgctgtt cgtgcacggc actctcaagg aaaaggctgt
     1741 ctggctgaaa ttcggcctgt agccgtcagg cggcgccggt gaagccgctt tcgccctgcg
     1801 ccttgcgcgc ggcatagacg cccgcgaagt cgatcgggtc cagctggaac ggcgggaagc
     1861 cgccctcggc ggtgacatcc gagatcagcc ggcgcgcgaa cgggaacaga tagcgcgggc
     1921 attcgatcat cagcaccgcc tcgatctcgc tgtcgggcac gccggtgacc tggaacaggc
     1981 cgccgtagag cagctcgaca tggaaggcca cggacgggcc gcgcttggcc tgggccgaca
     2041 gcttcaggtc cacctcgaac aggccgtcgg gacggccgcg cgcgctcatc tcgactccga
     2101 tgtcgatctg cggctgcagg ccctggctgt ccgaccggag cgattccggc gccagggggt
     2161 tctcgaatga caggtcgcga acgaactggg cgagaatgcg gaacccgggc tgggagatgt
     2221 cctggccgtt ctcggcgggc ggggtggtgg acgtatcgct catgaggaga tcgtgttcct
     2281 ggacatcttc ggacggccgc ccttcgtcag cgggcgggac atgatgaagg cgcgctgcta
     2341 tcacgcagcc ccagcccgcg caacgccggg ccccttacgc gggcgtcagc ctcgcctata
     2401 ttgcccatca acgacgtcag ccatcccggt ccccaagtga gatttctgga actcctcatc
     2461 ttcgccttct tcgccgccgt cgtgctgtac cagctctacg cggtgctcgg ccgccgcatc
     2521 ggccgtcagc cggaggacct acccgcggcg ggccccgccg ccgacggcga gcggccggcg
     2581 gtcaccgatg cgcccgaggc cgagcgggtg gacgtgtcgg gcctggcggc ggtccgggcc
     2641 cgcgacccgt cgttcgacgt ggcccatttc ctctccggcg cccgtcaggc ctatgagatg
     2701 atcgtcatcg ccttcgccgc cggcgaccat gcgaccctga agaacctgct cgccccggcc
     2761 gtggcggaga gtttcgaggc cgccatcgcc acgcgtgagg cggaaggtcg caccgagcgg
     2821 gtcgagttcc tgcagccccc ccgcgccgat ctcgagcgca tcgaggtcga cggcggcgtg
     2881 gcccgcgccg tcgtgcggtt cctgtccgag ttccgctccc gcaccaaggg ccccgagggt
     2941 gaggcggtcg acgatcgccg caccgccgaa ctgtggacat tcgaacgcaa tcttaacagc
     3001 cgcgacccca actggacgct tgttcacgtg gacgccgcgg aggcgtaatg cgatttcgag
     3061 ctatcggcgt cctgtgcgcg ctggcccttg cgggctgcgc cacgccgaag gcgggcccgc
     3121 cgctggtcgc cgtttccccc cctgctcctc cgcccgcggc cgaaacaccg gccgttccgc
     3181 cggcgccccc gcaggcttcg ggtttcgcgg ggttgcccgg ttgggacgcc gaggatcacg
     3241 ccgcggccct ggcagccttc agggcgacct gcaatgtcgc gcgggacgtc gcggcgagcg
     3301 cggtctgccg cagggccagg gcgctcggcc cgctcgacga cgccggcgcg cgccggtttc
     3361 tggaagccaa cttccggcca gaaccgctgg ccgagagtgg cctgctgacc gcctatttcg
     3421 ccccggtcta cgaggcctcg cccgtgcgac gcggcgcctt caccgcgccg gtccgggccc
     3481 ggcccgcgga ccttgtgatc accgacctgt ccgagatcga cgccggcgct ccctccggcc
     3541 agaccctggt cggacggctg gaggacggcc gcatggagcc ctatcccgat cgcgccacga
     3601 tcgaacgcag gggcgtgggc cgggtgctcg cctggatgcg gccggaagac ctgttcttcc
     3661 tgcagatcca gggttcaggc gtgctgacct tcccggacgg ttcgcgccgc cgcgccgcct
     3721 tcgcggccca caacggacat ccgttcgccg gcatcgccag gcccatgcgc gagaagggcc
     3781 tgctcaggga ctccgacacc tccggcgacg cgatccgggc ctggctggcc gctcatcgcg
     3841 gccgggaggc tgacgcgatc atgcggctca atccgcgcta tgtcttcttc accctgaccg
     3901 acgacgacgg ccgcgatcca gcgggcgccg ccggtgtgcc gctgccg
//
LOCUS       DAORNX010000282         3928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17267_length_3928_cov_9.113607, whole genome shotgun sequence.
ACCESSION   DAORNX010000282 DAORNX010000000
VERSION     DAORNX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17267_length_3928_cov_9.113607"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLO65_11835"
     CDS             complement(<1..195)
                     /locus_tag="PLO65_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRD28982.1"
                     /translation="MALITTVAVLLAACTTFMIQQWAVSRQETKAVSAAMNAVVAQIA
                     APALARGDEAAARRAVGSLIA"
     gene            408..749
                     /locus_tag="PLO65_11840"
     CDS             408..749
                     /locus_tag="PLO65_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28983.1"
                     /translation="MEKAFVVQRVARKLFATERSLDATMTETAELMAEFVKARQDLKL
                     SAVVGDAATAKVIEALAALGEARTAIVTAHAELDDVRLRIGVRTKMAGWEDKPPESDQ
                     PKNHQDLRQVS"
     gene            812..1246
                     /locus_tag="PLO65_11845"
     CDS             812..1246
                     /locus_tag="PLO65_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28984.1"
                     /translation="MYHSPLPLIGWGATLLVCALAWWRGDKAERYGSLIIVVAGLAVF
                     LSHSSLPAPAASLSLLIVDAGMAAGFLLLAIRYASLWLGSAMLLQAGQFSLHAFYLVG
                     DLEHDRFYAVANNLITVGILICILVGTGISWRRRSRTGSQTA"
     gene            complement(1234..2139)
                     /locus_tag="PLO65_11850"
     CDS             complement(1234..2139)
                     /locus_tag="PLO65_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD28985.1"
                     /translation="MQSRARMDWVVLGVLVIIWGSAFAGLKIAVGGIHPFWVSAIRLW
                     VATGTLWIISAMRGEQLPPAGIGRDSPWPAYAMLGLVGFALPFFMFSTAAKSLPSAVN
                     AICNGGSPVFTVVMAHLFLQDERLTPRRLLGVGLGFAGLMALVWPRLSSGASLEAGAL
                     GLAIGAAVFYGASNIMARKAPQVSSLTGGLMMCLTGAVFATAGALALSGLPSPPPMGP
                     LLVTVVLGVFSSGLGTVGWVWLIQRRGAVFTSMTVYLLPIWAMVLGIGLMGERPGWSA
                     FAALALILAGVGLTTARPKAAVQAV"
     gene            2316..3491
                     /locus_tag="PLO65_11855"
     CDS             2316..3491
                     /locus_tag="PLO65_11855"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRD28986.1"
                     /translation="MTTTPQKPDLRPARPEFSSGPCAKRPGWTPQSLEDAVLGRSHRG
                     KPGKAKLKLAIDHTRAVLGVPADYLVGIVPGSDTGAVEMAMWSLLGPRPVQLLAFESF
                     GKDWVTDVTKQLKLPDVEVLDAPYGQLPDLTRVRPDADLVFTWNGTTSGVRVPNADFI
                     AADREGVVICDATSAAFAQDLDWAKLDVVTFSWQKALGGEGAHGMLILSPRAVARMES
                     YTPPWPMPKLFRMTKGGKVSLDIFEGATINTPSMLCVEDYLDALDWADAQGGLAGLRR
                     RADANFAVIEAWVARTPWVDFLAEAPETRSNTSVCLKVTDPAIAALPAEAQADVAKRL
                     ASMLEKAGVAYDVGAYRDAPPGLRIWCGCTIETADLEALTPWLDWAFASVSAELAAA"
     gene            3592..>3928
                     /locus_tag="PLO65_11860"
     CDS             3592..>3928
                     /locus_tag="PLO65_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRD28987.1"
                     /translation="MAPRVLIADKLSPAAVEIFRNRGVDFDIKTGLTKDELIAVIGDY
                     DGIAIRSGAKLDKDVIAAAHKLQVIARAGIGVDNVDIPAATAKGVVVMNTPFGNSITT
                     AEHAIAMMFA"
BASE COUNT          674 a         1415 c         1255 g          584 t
ORIGIN      
        1 ggcgatcagc gatccgacgg cgcgccgcgc ggcggcttcg tcgccgcggg ccagggccgg
       61 ggcggcgatc tgggccacca cggcgttcat cgctgcgctg accgccttgg tctcctgtcg
      121 tgagaccgcc cactgctgga tcatgaacgt ggtgcaggcc gccagcagca ccgccactgt
      181 cgtgatcagc gccacgcttg ccacgcgagc ctggaacggt ccgtggctcg aggcctgtga
      241 tgtgtccgtc agctggctca tccgcgcgaa aaccacccct aagcgggccc ttatcgcgca
      301 atctccctaa cgcttcgctt ccgcgacaat ttaaccatct atttacctag ccagaaatta
      361 aggtttcgtt taccttgatt acgcctaccg gttgatggag ggttcaggtg gaaaaagcat
      421 tcgtagtcca acgcgtggcc aggaaactgt tcgcgaccga gcgttcgctc gacgcgacga
      481 tgaccgaaac cgcggaattg atggcggagt tcgtgaaggc ccgtcaggac ctcaaactct
      541 ccgcggtggt cggcgacgcc gccacggcca aggttatcga ggccctcgcg gcgctgggcg
      601 aagcccgcac cgccatcgtc acggcgcacg cggaactcga cgacgtccgg ctgcgcatcg
      661 gcgtgcgcac gaagatggcc ggctgggaag acaagccgcc ggagtccgac cagccgaaga
      721 accatcagga ccttcgccag gtcagctgac cggccaaggt tgcgatagtt taactctctt
      781 gcgcctctaa ctccgtgtag aggcgcaaga gatgtatcac tcaccactcc cattgatcgg
      841 ctggggcgcc acgctgctgg tgtgcgccct agcgtggtgg agaggtgaca aggccgaacg
      901 atacggctcg ctgatcatcg tggtcgccgg cctggcggtt ttcctctcgc actccagtct
      961 tcctgcgccg gccgcgagcc tgtcgctgct gatcgtcgat gcgggcatgg cggcagggtt
     1021 cctgctactc gccattcgct atgcgagcct gtggctggga tcggcgatgc tcctgcaggc
     1081 cggtcaattc agcctgcatg cgttctatct ggtcggagac cttgagcacg accggttcta
     1141 cgccgtggcc aacaacctga tcaccgtcgg cattctcatc tgcattctcg tcggcacggg
     1201 catatcctgg cgccgccggt cgagaaccgg gtctcagacc gcctgaacgg cggctttcgg
     1261 ccgggcggtg gtcaggccga ccccggcgag gatcagggcc agcgccgcga acgcgctcca
     1321 gcccggccgc tcgcccatca gcccgatccc cagcaccatg gcccagatcg gcagcagata
     1381 gacggtcatg ctggtgaaca ccgcgccgcg ccgctgaatc agccagaccc agcccaccgt
     1441 gcccaggcca ctggagaaga cgccgagcac cacggtgacc agcagcggtc ccatcggcgg
     1501 cggcgacggg aggccgctca acgccagcgc gcccgccgtg gcgaacaccg ccccggtcag
     1561 gcacatcatc aggccgccgg tgagcgaact gacctgtggc gccttgcggg ccatgatgtt
     1621 ggacgcgccg tagaagaccg ccgccccgat cgccagcccc agggcgccgg cctccagcga
     1681 ggcgcccgag gacaggcgcg gccagaccag ggccatcagc ccggcgaatc cgaggccgac
     1741 gcccagcagc cgtcggggag tcagccgctc gtcctgcagg aagagatgcg ccatcaccac
     1801 ggtgaacacc ggggagccac cattgcagat ggcgttgacg gcgctcggca ggctcttcgc
     1861 cgccgtcgag aacatgaaga acggcaaggc gaatccgacc aggccgagca tggcgtaggc
     1921 cggccagggc gagtcgcggc cgatgccggc cggcgggagc tgctcgccgc gcatggcgct
     1981 gatgatccac agcgtcccgg tggcgaccca caaccggatg gccgagaccc aaaaggggtg
     2041 gatgccgccc acggcgatct tcagcccggc gaaggccgag ccccagatga ttaccaggac
     2101 ccccagcacc acccagtcca tgcgcgctcg agactgcacc cggttccccc cgccagaccc
     2161 cggacctaag gccgcgtgcg gcgacacgcc agcgcttcct gtccgtggca aaagaaaatc
     2221 tcctcttgtg cgacgcagcg aaatcgggtt cttcgacagc gggccacgcc ctcccaacgg
     2281 ggcgcagtcc atctgcaagg atgggagaca tcgcagtgac cacgactccg cagaagccgg
     2341 atttgcgccc ggcgcgcccc gaattctctt caggcccctg cgccaagaga cccggctgga
     2401 ccccacaatc cctcgaagac gccgtccttg gacggtcgca tcgcggcaag cccggcaagg
     2461 cgaagctgaa gctggcgatc gaccacaccc gcgcggtgct cggcgtgccg gccgactatc
     2521 tggtcggcat cgtgcccggc tccgacaccg gcgccgtcga gatggcgatg tggtcgctgc
     2581 tgggccccag gcccgtgcag ctgctggcct tcgaatcgtt cggcaaggac tgggtcaccg
     2641 atgtcaccaa gcagctgaag ctgcccgacg tcgaggtgct cgacgccccc tatggccagc
     2701 tgccggacct gacgcgcgtc cgccccgacg ccgacctggt gttcacctgg aacggcacca
     2761 ccagcggcgt gcgcgtgccg aacgccgact tcatcgccgc cgaccgcgag ggcgtggtga
     2821 tctgcgacgc gaccagcgcc gccttcgccc aggacctcga ctgggcgaaa ctcgatgtgg
     2881 tgaccttctc ctggcagaag gcgctcggcg gcgagggcgc gcacgggatg ctgatcctgt
     2941 cgccgcgcgc cgtggcccgg atggagagct acacgccgcc gtggccgatg cccaagctgt
     3001 tccgcatgac caagggcggc aaggtcagcc tcgacatctt cgagggcgcg acgatcaaca
     3061 ccccctcgat gctctgcgtc gaggactatc tcgacgcgct ggactgggcc gacgcccagg
     3121 gcggactggc cggactgcgc cggcgggccg acgccaactt cgcggtcatc gaggcctggg
     3181 tcgcccgcac gccgtgggtc gacttcctgg ccgaggcgcc cgagacgcgc tccaacacca
     3241 gcgtctgtct caaggtcacc gatccggcca tcgccgccct gccggccgag gcccaggccg
     3301 acgtcgccaa gaggctggcc tcgatgctcg agaaggcagg cgtcgcctac gacgtcggtg
     3361 cctaccgcga cgccccgccg ggtctgcgca tctggtgcgg ctgcacgatc gagacggccg
     3421 atctcgaggc cctgacgcca tggctcgact gggccttcgc gtccgtctcc gccgaactgg
     3481 ccgccgcctg atccctcgtc cccggcccgc ccggccgggg acgccccctc cccttcgcat
     3541 tgagccggga cgaccgcgac gcggcgaccg cgctccagag gaacacagcc catggctccc
     3601 cgcgttctca tcgctgacaa gctcagcccc gccgccgtcg agatcttcag gaatcgcggc
     3661 gtcgacttcg acatcaagac cggcctgacc aaggacgaac tgatcgccgt catcggcgac
     3721 tacgacggca tcgccatccg ctcgggcgcc aagctcgaca aggacgtgat cgccgccgcc
     3781 cacaagctgc aggtcatcgc ccgcgccggc atcggggtcg acaatgtcga tatcccggcc
     3841 gccaccgcca agggcgtggt ggtgatgaac actccgttcg gcaactcgat caccaccgcc
     3901 gagcacgcca tcgccatgat gttcgccc
//
LOCUS       DAORNX010000283         3909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17380_length_3909_cov_8.358848, whole genome shotgun sequence.
ACCESSION   DAORNX010000283 DAORNX010000000
VERSION     DAORNX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17380_length_3909_cov_8.358848"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..2051)
                     /gene="acs"
                     /locus_tag="PLO65_11865"
     CDS             complement(111..2051)
                     /gene="acs"
                     /locus_tag="PLO65_11865"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRD28988.1"
                     /translation="MSDGQVFPVPQAWAERARMDAKAYEAACARVESDPDGFWREVAG
                     RLDWVKPFTEVKDVSWNREDFRIRWFADGVLNVSVNCLDRHLPTRADDVAFIWEGDDP
                     ADSKKITYREAHEEVCRMANVLKAHGVKKGDRITIYLPMIPEAAYAMLACSRIGAVHS
                     VVFGGFSPDSLAGRILDCDSRVVITADEGVRGGRIVPLKMNVDLALTQCPDVETVLVI
                     RRTGAKWLEHNGRDVAYEPERERVSNVCPPEPMNAEDPLFILYTSGSTGKPKGVLHTT
                     GGYLVWASWTHELVFDYRPGEIFWCTADVGWVTGHSYIVYGPLANGATSLIFEGVPNY
                     PTTSRFWEVIDKHQVEIFYTAPTALRALMREGEEPVKKTSRKSLRLLGSVGEPINPEA
                     WLWYHRVVGEGRCPIVDTWWQTETGACLVSPLPGATPLKPGSASKPLPGVKLQLVDAE
                     GLVLEGATSGNLCLTDSWPGQMRTVYGDHQRFIDTYFTTYPGKYFTGDGCRRDEDGYY
                     WITGRVDDVINVSGHRMGTAEIESALVSHHDVAEAAVVGFPHDIKGQGIYAYVTLNAD
                     TTPSEELRAALVKWVRREIGPFAAPDAIQWAPGLPKTRSGKIMRRILRKVAENDLSNL
                     GDTSTLADPAVVDDLVANRITR"
     gene            2135..2884
                     /locus_tag="PLO65_11870"
     CDS             2135..2884
                     /locus_tag="PLO65_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRD28989.1"
                     /translation="MLLAKSTWPEIEAFLQRSTSVVVPIGSNEQHGPTGLLGTDWMCP
                     EIIAHEAQKGRDDLLVAPTFNIGMAQHHLGFPGTISLRPSTFMAAIGDWCRSLAGHGF
                     TKIYFLNGHGGNTHTIEAAFSELYAEASFAGRPRGFACKLKNWWELSGVNGLASRQFP
                     VGHGSHATPSEIAVTQWAYPDAIKAASYAPQIAPTGPIREALDFRARYADGRMGSDPA
                     QATPEKGGELVALAARGLIEDLAAFGAEIAP"
     gene            complement(3035..3394)
                     /locus_tag="PLO65_11875"
     CDS             complement(3035..3394)
                     /locus_tag="PLO65_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRD28990.1"
                     /translation="MEGLNPARPAAGVLLCRLDEIPDPGSRAFHFREDDKLFLGFVVR
                     RGERLYGFVDSCPHTGQPLAGPTGRYATRENDLILCSGHGALFRIGDGMCTSGPCEGQ
                     RLAVWAVEVREGQVVTA"
     gene            complement(3382..3672)
                     /locus_tag="PLO65_11880"
     CDS             complement(3382..3672)
                     /locus_tag="PLO65_11880"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD28991.1"
                     /translation="MPLFIVQWKDRVPEGPAIRAATREAHLAWAAAQPLKIKLGGPFL
                     TADEGMAGSLIIVEAPDLAAAQAFHADDPYTRAGLYESSSVEPWRVTIPWKD"
     gene            complement(3682..>3909)
                     /locus_tag="PLO65_11885"
     CDS             complement(3682..>3909)
                     /locus_tag="PLO65_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28992.1"
                     /translation="QRQAEAQRRQALEQALAGKLTVAEGVASAPAVRALARRLGVETP
                     ISEAVAAILAGEVGVDDAILGLLSRPLKAER"
BASE COUNT          584 a         1354 c         1361 g          610 t
ORIGIN      
        1 aggcgtcggg cgcccagtcg gcccgggccg cgccggcggc gagcgccagg cccgtcggca
       61 ggaagacggc gaggcgcttc agccagcgga tcgccccggc ggccatgccg tcaccgcgtg
      121 atgcggttgg cgaccagatc gtcgaccacc gccggatcgg cgagggtcga ggtgtcgccg
      181 aggttcgaca gatcgttctc ggccaccttg cgcaggatgc ggcgcatgat cttccccgag
      241 cgcgtcttgg gcagaccggg ggcccactgg atggcgtccg gcgcggcgaa cgggccgatc
      301 tctctgcgga cccatttcac cagcgccgcc cgcagctcct ccgacggcgt cgtgtcggcg
      361 ttcagggtga cataggcgta gatgccctgg cccttgatgt cgtgcgggaa gccgaccacg
      421 gcggcttcgg ccacatcatg gtgtgagacc agggccgact cgatctcggc cgtgcccatg
      481 cggtggcccg agacgttgat cacgtcgtcg acccggccgg tgatccagta gtagccgtcc
      541 tcgtcgcgcc ggcagccgtc gccggtgaag tacttgccgg gataggtggt gaaataggtg
      601 tcgatgaagc gctggtggtc gccatagacc gtgcgcatct ggcccggcca gctgtcggtc
      661 aggcaaaggt tgccgctggt cgcgccctcg agcaccaggc cctcggcgtc gaccagctgc
      721 agcttcaccc ccggcagggg cttgctggcc gagccgggct tgaggggcgt ggcgccgggc
      781 agggggctga ccaggcaggc gcccgtctcg gtctgccacc aggtgtcgac gatcgggcag
      841 cggccctcgc cgaccacgcg gtggtaccac agccaggcct ccggattgat cggctcgccg
      901 acgctgccga gcaggcgcag gctcttgcgc gaggtcttct tcaccggctc ctcgccctcg
      961 cgcatcagcg cccgcaaggc cgtgggggcg gtgtagaaga tctccacctg atgcttgtcg
     1021 ataacctccc agaaacggct ggtggtcgga tagttgggca ccccctcgaa gatcaggctg
     1081 gtcgcaccgt tcgccagcgg gccgtagacg atgtagctgt ggccggtgac ccagcccacg
     1141 tccgcggtgc accagaagat ctcgccgggc cggtagtcga acaccagttc gtgggtccag
     1201 ctggcccaga cgagatagcc gcccgtggtg tgcagcaccc ccttgggctt gccggtcgag
     1261 cccgaggtat agaggatgaa cagcggatcc tcggcgttca tcggctcggg cgggcagacg
     1321 ttggacaccc gctcccgctc gggctcgtag gccacatcgc ggccgttgtg ttcgagccat
     1381 ttggccccgg tgcggcggat gaccagcacc gtctccacgt ccgggcactg ggtcagggcc
     1441 agatcgacgt tcatcttcag cggcacgatc cgcccgcccc gcacgccttc gtcggcggtg
     1501 atcaccaccc ggctgtcgca atcgagaatc cggccggcca ggctgtcggg ggagaagccg
     1561 ccaaagacca cggagtggac cgcgccgatg cgtgaacagg ccagcatggc gtaggccgcc
     1621 tcggggatca tcggcaggta gatcgtgatc cggtcgccct tcttcacgcc atgcgccttg
     1681 aggacgttgg ccatgcggca cacttcctcg tgcgcctcgc gataggtgat cttcttcgag
     1741 tctgcgggat cgtcgccttc ccagatgaag gccacgtcgt cggcgcgggt cggcagatgg
     1801 cgatcgaggc agttgaccga gacgttcagc accccgtcgg cgaaccacct gatacggaaa
     1861 tcctcgcggt tccaggagac gtccttgacc tcggtgaacg gcttgaccca gtcgagccgg
     1921 ccggcgacct cccgccagaa accgtccggg tcgctctcca cccgggcgca ggcggcctcg
     1981 taggctttcg cgtccatgcg ggcgcgctcg gcccaggcct gtggaaccgg aaacacctgc
     2041 ccgtcgctca cgtccgtctc ccttgccttc ttgttgggcg cgagtatgcg gatggcggtc
     2101 ggcggcgcaa ggatgccgct gcgggagttt tccaatgttg ctcgccaaat ccacctggcc
     2161 cgagatcgag gccttcctcc agcggtcgac cagcgtggtc gtgccgatcg gctcgaacga
     2221 gcagcacggg cccacgggcc tgctcggcac cgactggatg tgtccggaga tcatcgccca
     2281 cgaggcccag aagggccgcg atgacctgct ggtcgccccg acgttcaata tcggcatggc
     2341 ccagcatcac ctgggcttcc cgggcaccat cagcctgcgg ccctcgacct tcatggcggc
     2401 gatcggcgac tggtgccgct cgctggccgg gcatggcttc acgaagatct atttcctcaa
     2461 tggccacggc ggcaacacgc acaccatcga ggcggcgttc agcgaactgt acgccgaggc
     2521 cagcttcgcc ggcagaccgc gcggcttcgc ctgcaagctc aagaactggt gggagctcag
     2581 cggcgtgaac ggcctggcca gccgccagtt tccggtcggc cacggcagcc acgccacccc
     2641 gtcggagatc gccgtgaccc agtgggccta cccggacgcg atcaaggccg ccagctacgc
     2701 gccgcagatc gccccgaccg gcccgatccg cgaggccctc gacttccgcg cccgctacgc
     2761 cgacggccgc atgggctcgg acccggccca ggcgacgccg gaaaagggcg gcgagctggt
     2821 cgccctggcc gcgagggggc tgatcgagga cttggccgcg ttcggggcgg agatcgcgcc
     2881 gtagaccctt ctccccttgc gggagaaggt gtcaggcgga gcctgacgga tgaggggtct
     2941 tgccggtggc tctggaggtc aagcggatgg gggcatcaac ccctcttccg acccgcttcg
     3001 cgggccacct tctcccgcaa ggggagaagg accctcaagc cgtgaccacc tgcccctcac
     3061 gcacctcgac cgcccacacc gccagccgct gcccctcgca cggcccgctc gtgcacatcc
     3121 cgtccccgat cctgaacagc gcgccgtggc cggagcagag gatcaggtcg ttctcgcgcg
     3181 tcgcgtaccg gccggtggga ccggccaggg gctgtccggt gtgcgggcag ctgtcgacaa
     3241 agccgtagag ccgctcgccc cgccggacca cgaagccgag gaacagcttg tcgtcctccc
     3301 ggaagtggaa tgcccgcgag ccgggatcgg ggatttcgtc gagccggcag agcaggacgc
     3361 cggcggccgg tctggccggg ttcagtcctt ccacgggatg gtgacccgcc agggctcgac
     3421 gctggagctc tcgtagagcc cggccctggt atagggatcg tcggcgtgga aggcctgcgc
     3481 ggcggcgagg tccggggcct cgacgatgat cagcgagccg gccatgccct cgtcggcggt
     3541 gaggaacggc ccgcccagct tgatcttcag cggctgggcc gcggcccagg ccagatgcgc
     3601 ctcgcgggtg gcggcgcgga tcgccgggcc ctcgggaacc ctgtctttcc actggacgat
     3661 gaagagcggc atggatctgt cctaacgttc ggctttgagg gggcgcgaga gcaggccgag
     3721 gatggcatcg tcaacgccga cctcccccgc gagaatggcg gcgacggctt cactgatcgg
     3781 tgtctcgacg cccagccggc gggccagggc gcggacggcg ggggccgagg cgacgccctc
     3841 ggcgacggtc agcttgccgg ccagggcctg ctccagcgcc tgacgtcgct gcgcttcggc
     3901 ctggcgctg
//
LOCUS       DAORNX010000284         3902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17431_length_3902_cov_7.021835, whole genome shotgun sequence.
ACCESSION   DAORNX010000284 DAORNX010000000
VERSION     DAORNX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17431_length_3902_cov_7.021835"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..133
                     /gene="rrf"
                     /locus_tag="PLO65_11890"
     rRNA            19..133
                     /gene="rrf"
                     /locus_tag="PLO65_11890"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            204..280
                     /locus_tag="PLO65_11895"
     tRNA            204..280
                     /locus_tag="PLO65_11895"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Met,seq:cat)
     gene            345..896
                     /locus_tag="PLO65_11900"
     CDS             345..896
                     /locus_tag="PLO65_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRD28993.1"
                     /translation="MSPASGTVIAVSRGQRHGLGKLNHRVIRLVAGLGVEGDVHAGVT
                     VRHRSRVARDPAQPNLRQVHLIHAELFDDLLTQGYSVTPGAMGENITTRDVDLLGLPA
                     GAVLCLGHAAAVEVTGLRNPCTQLDGLQTGLMRACQGRGANGEPVRKAGVMGVVITGG
                     EVHPGDGIVVRLPDGPQRALKPV"
     gene            1159..1959
                     /locus_tag="PLO65_11905"
     CDS             1159..1959
                     /locus_tag="PLO65_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD28994.1"
                     /translation="MSFITANGLDLWVERKGEGEPLLFISGSGGDLRNKPGPLDGPLA
                     RHFEMVAWDQRGLGQSDKPDGPYSMADYADDAAALLDELDWASANVVGVSFGGMVAQE
                     LAIRHPGRIRKLVLCCTSPGGEGGASYPLHTLIGMDPADQARKMIPISDTRHDAAWQA
                     DHPGAYQMMFDLRTSDPWAHEPRRAIGAELQLLARKDHDTWGRLPSIAAPTLVCGGRY
                     DGIALPQTQQRMAGRIPGAQLRMFEGGHMFLIQDRAAFPAIIDFLQAP"
     gene            1956..3548
                     /locus_tag="PLO65_11910"
     CDS             1956..3548
                     /locus_tag="PLO65_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD28995.1"
                     /translation="MTDAIIIGGGHNGLVCAFYLARAGLKVTVLERRDVVGGAAVTEE
                     FHPGFRNSVASYTVSLLNPRVIADLDLPRHGLKVINRKVSNFLPTRDGGYLATGGGRT
                     KEEVAKFSVRDAAALDAYNARLEAIADVLRELVLQSPPNMTQGSLAEAIPQLLKSAVL
                     GKRISNLDVTGRRDLLAMFSQSAGDWLDGWFESDPIKAVLGFDGIVGAFASPYTPGTA
                     YVLLHHVFGEVNGEKGAWGHAVGGMGAITQAMAAACREKGVEIRTGVPVAEVLTEQGR
                     AVGVVTDAGETLRARRVVANIHPQLLFQKLIDPALLPADFNERITRYRSGSGTFRMNV
                     ALSALPRFTCKPEPGDHLTGGIIIAPDLAYMERAYADARQHGWSKEPIVEMLIPSVVD
                     DSLAPAGQHVASLFCQHVQPVLSGGRSWDDHREEVADLMIDTVDKWAPGFKASVLGRQ
                     INSPLDLERTFGLVNGDIMHGVLSLDQLFSARPVLGHGAYRGPLKGLYLCGAGTHPGG
                     GVTGAPGHNAAREILRDARRRA"
     assembly_gap    3565..3578
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          609 a         1352 c         1294 g          633 t
ORIGIN      
        1 tcacatccct tccgccgacc tggtggctat gccgggggtt ccccacccga tcccattccg
       61 aactcggtcg ttaagtcccc cagggccaat ggtacttcgt ctcaaggcgc gggagagtag
      121 gtcgccgcca ggtccgccga agggatgcga atgatgatgt ccgccacttg acccttcata
      181 acgattactt acgtccggat tgccgcggga tggagcagcc cggtagctcg tcaggctcat
      241 aacctgaagg tcgcaggttc aaatcctgct cccgcaacca aattcaacaa acccgtcgga
      301 cccctgtctg gcgggttttg tcgtttccgg gctatgcctg tcgcatgagc ccagcgtccg
      361 gaaccgtcat tgccgtcagt cgcggccagc gccacgggct cggcaagctg aaccaccggg
      421 ttatccgcct ggtcgccggc ctgggggtcg agggcgatgt ccatgcaggc gtgacggtca
      481 ggcaccgctc ccgcgtggcc cgcgatccgg cccagcccaa cctgcgccag gtccacctga
      541 tccatgccga gctgttcgac gacctgctga cgcagggcta ttcggtcacg cccggcgcca
      601 tgggcgagaa catcaccacc cgcgacgtcg acctgctggg cctgcccgcg ggcgccgtgc
      661 tgtgtctggg ccacgccgcc gcggtcgagg tcaccggcct gcgcaacccc tgcacccagc
      721 tggatggcct gcagaccggc ctgatgcggg cctgccaggg ccgcggcgcc aacggcgagc
      781 cggtgcgcaa ggcgggggtg atgggggtgg tgattaccgg cggggaggtc catccgggtg
      841 acgggatcgt ggtccggctg cccgatgggc cgcagagggc gctcaagccg gtctgagcac
      901 catcaggtcg acacgacggt ccagccctga tgtcttgacg gccattcttg ctccggaggg
      961 agacctgctc aaggaccgct tcgcggccgc agagcggcgg ccccgcaggg gccgtccttg
     1021 accaggactc ccgtccggag cgcatcattc catcaaggtc ttcagggcca tttgtcgtcc
     1081 gggcgccttg ggcggtgtcg cgaacgacac tttccgtcgc cccctttccc ccgcggcccc
     1141 gccgtgagag atcaggccat gagcttcatc acggccaatg gcctcgatct gtgggtcgaa
     1201 cggaagggcg agggcgagcc cctgctgttc atttccggca gcggcggcga cctgcgcaac
     1261 aagccgggcc cgctcgatgg cccgctggcc cgtcatttcg agatggtcgc ctgggaccag
     1321 cgcggtctcg gccagagcga caagcccgac ggcccctaca gcatggccga ctacgccgat
     1381 gacgccgccg ccctgctcga tgagctggac tgggcctcgg ccaacgtcgt cggcgtgtcg
     1441 ttcggcggca tggtcgccca ggagctggcc atccggcatc cagggcgtat ccgcaagctg
     1501 gtgctctgct gcacctcgcc gggcggcgag ggcggggcgt cctacccgct gcacaccctg
     1561 atcggcatgg atccggcgga ccaggcccgc aagatgatcc ccatttccga cacgcgccac
     1621 gacgccgcct ggcaggccga ccatcccggg gcctaccaga tgatgtttga cctgcggacc
     1681 agcgatccct gggcgcacga gccgcggcgg gccatagggg cggagctgca gctgctcgcc
     1741 cgcaaggacc acgatacctg gggccggctg ccgtcgatcg ccgccccgac cctggtctgt
     1801 gggggccggt acgacggcat cgcgcttcca cagacccagc agcgcatggc cgggcgcatc
     1861 ccgggcgcgc agctgcgcat gttcgagggc ggccacatgt tcctgatcca ggaccgcgcc
     1921 gcctttcccg ccatcatcga cttcctgcag gccccatgac cgacgccatc atcatcggag
     1981 ggggtcacaa cggcctcgtc tgcgctttct atctggcccg ggcggggctg aaggtcaccg
     2041 tgctcgagcg ccgcgatgtg gtcggcgggg cggcggtgac cgaggaattc caccccggtt
     2101 tccgcaattc cgtggccagc tacacggtca gcctgctaaa tcccagggtc atcgccgacc
     2161 tggacctgcc ccgtcacggg ctgaaggtga tcaatcgcaa ggtctccaac ttcctgccca
     2221 cccgcgacgg cggctatctc gccaccggcg gcggccgcac gaaggaggag gtcgcgaaat
     2281 tctcggtgag ggacgccgcg gccctcgacg cctacaacgc ccggcttgag gcgatcgccg
     2341 acgtgctgcg cgaactggtg ctgcaaagcc cgcccaacat gacccagggc agcctggccg
     2401 aggcgatccc gcagctgctc aagagcgccg tgctgggcaa gcgcatctcc aacctcgacg
     2461 tcaccggccg gcgcgacctg ctggcgatgt tttcccagtc ggccggcgac tggctggacg
     2521 gctggttcga gagcgacccg atcaaggcgg tgctgggctt cgacggcatc gtcggcgcct
     2581 tcgccagtcc ctatacgccc ggtacggcct acgtcctgct gcaccatgtg ttcggggagg
     2641 tgaacggcga gaagggggcc tggggccacg ccgtcggcgg catgggcgcg atcacccagg
     2701 cgatggccgc cgcctgtcgc gaaaagggcg tagagatccg caccggagtc ccggtcgctg
     2761 aggtgctgac cgaacagggg cgggccgtgg gcgttgtcac cgacgccggt gaaacgctcc
     2821 gcgccaggcg ggtcgtcgcc aacattcacc cccagctgct gttccagaag ctcatcgacc
     2881 ccgccctgct gccggcggac ttcaacgagc ggatcacccg ctatcgttcg ggatccggca
     2941 ccttccgcat gaatgtggcc ttgagcgcgc tgccacgatt cacctgcaag ccggagcccg
     3001 gtgatcacct gaccggtggc atcatcattg cgccggacct ggcctacatg gagcgcgcct
     3061 acgccgacgc gcgccagcac ggatggtcga aggaaccgat tgtcgagatg ttgatccctt
     3121 cggtggtcga cgacagtctg gccccggccg gccagcacgt ggccagcctg ttttgccagc
     3181 acgtgcagcc ggtcctgtcc ggcggccgca gctgggacga ccaccgcgag gaggtcgccg
     3241 acctgatgat cgacacggtc gacaaatggg ccccggggtt caaggcctcg gtgctcggtc
     3301 gccagatcaa cagtccgctc gacctcgagc gcaccttcgg tctggtcaat ggcgacatca
     3361 tgcacggcgt gctgagcctg gaccagctgt tcagcgcccg gcctgtgttg gggcatggcg
     3421 cctatcgcgg ccccctgaag ggcctctacc tttgcggggc ggggacccat cccggcggcg
     3481 gcgtcaccgg cgcgcccggc cacaacgcgg cgcgggaaat cctgcgggac gcgcgccgtc
     3541 gcgcctgacg atgcggcgcg ggggnnnnnn nnnnnnnngc cggttgctct cgcgggcgtt
     3601 gttgttgcga gctgttcgaa cccggtcgtt agtcacaaaa caggcaatat ctggccacgg
     3661 accggacaat tcgtcgcggt tactgactca tgtctgtttt cggcctccgc ccccctttca
     3721 ggtaacgccg ttcggcgcca taggcttgcg ccgcgtccgg aagcagtact tcggaccggc
     3781 atagaggacg agcatgttcg agaccctgtc tggcggcgag acgttccggc gcgaggtcgc
     3841 caccgaagtc gaatacctga cgaaaatgca cggggccgac gcgctgaagg tcgcccgcga
     3901 ga
//
LOCUS       DAORNX010000285         3898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17453_length_3898_cov_7.645329, whole genome shotgun sequence.
ACCESSION   DAORNX010000285 DAORNX010000000
VERSION     DAORNX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17453_length_3898_cov_7.645329"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1852
                     /gene="murJ"
                     /locus_tag="PLO65_11915"
     CDS             236..1852
                     /gene="murJ"
                     /locus_tag="PLO65_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRD28996.1"
                     /translation="MKPAKGGLIRSSMVFSSLTLVSRFMGLARDLFITARLGASSTPA
                     ADAFNTAQSFPNLFRRIFAEGAFTAAFVPTYAKVLAAEGQEAADNLATDALATLAAIT
                     LVLTVAFQLAMPWLMLAINPGFGFNSEKYRLAVVLTQISMPYLPCMAIVALLSGVLNA
                     GGRFALSAAAPTVLNAVILIAVIPPRDAIGSAYAATWAIFAAGLVQAGLLYWGARRTG
                     AKIRPTIPRLTPEIRALIAVAIPGAVAASVTQINIFLSGIIASQVNGARTWLSVADRF
                     YQLPLGLVGVAIGVALLPSLSRAIGLGDKVEAQRTTDQAIVFAMALTLPAAAVLMAMP
                     HYLIDGLFQRGEFSPYDASETAKALFHYGWGVPAFVLARVLSPVFFARHDTRAPMRFA
                     LISVGVNIGLGLVLFYTIGFEGIAAATAFASWLNVAQMVHALRRRGDWTPSPAAWSKL
                     ARIAMASGLLGAAMFAAQLFRAGLEAPLAGVRVIGLGPKEITVLALCLAGAALYPLLL
                     LGSGGVTLAEIREALRRKPGAAAAPPSDLP"
     gene            1996..3027
                     /gene="trpS"
                     /locus_tag="PLO65_11920"
     CDS             1996..3027
                     /gene="trpS"
                     /locus_tag="PLO65_11920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRD28997.1"
                     /translation="MTDQPPAPYAGPQRVLSGVQPSGALHLGNYLGALVKFARLQHEQ
                     ECLFCVVDLHAITVWQDPAQLTAQTREIAAAYIAAGVDPAKAGIFPQSAVGAHAELAW
                     ILQCVARLGWLDRMTQFKEKSGKHKERSSVGLYTYPVLQAADILVYKATHVPVGEDQK
                     QHLELTRDIAAKFNHDFDRPGFFPLPDGLIEGPGARIMSLRDGTAKMSKSDPSDNSRI
                     NLTDDADAIAQKIRKAKTDPEPLPESLDDLKARPEADNLVGIYGALAGLTRAQVLAEF
                     GGKGFGVFKPALADLAVEKLGPVSDRMRRLMADPAEIDRVLAAGADKAREVAAPTLDE
                     VKRIVGFWR"
     gene            3143..3652
                     /locus_tag="PLO65_11925"
     CDS             3143..3652
                     /locus_tag="PLO65_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD28998.1"
                     /translation="MLSTASSATVAPMSQRKFLVIADDSPESGAALRYACRRARSTGG
                     HVCLLRVIEPAVFEHWSGVREEMERQERVEAEMVLQKLAETVVAVTGKAPEFLIKHAE
                     NTKSAIRSVVANDPDIKILVLAASTGGKGPGPLVASLARDGVNWGARKLPVTVVPGDL
                     TDEEIADLA"
     gene            3649..>3898
                     /locus_tag="PLO65_11930"
     CDS             3649..>3898
                     /locus_tag="PLO65_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD28999.1"
                     /translation="MSGEPGFTPSSPLTRKPRKRSWLFAWVLLALVAGMTLIVLAGPR
                     VSPAAWRAAGAAYGVLLAFMAVRQVWLIRRDLRSRRHRD"
BASE COUNT          548 a         1426 c         1322 g          602 t
ORIGIN      
        1 ctcaaggcgg cgctgatgga ggtgctcgag gccgaggacg gcggccagac cccgcgcggc
       61 cggccgaggc tcgagcgggc gcgggccagc gtcggccggt agtcgtcctt ccgacccgtt
      121 tcgcggcgag ggccgagatt ccatggagtt tccgcgcacg tcgccgccgc tccttgaccg
      181 ttccccccgg ccagcgcact aacgccgggt gagcgtcgag cagccgtcca gacccgtgaa
      241 acctgcgaag ggcggcctga tccgctcgtc gatggtgttt tccagcctga ccctggtcag
      301 ccgcttcatg ggcctggccc gcgacctgtt catcaccgcc cggctgggcg ccagctcgac
      361 cccggcggcc gacgccttca acaccgccca gtccttcccc aacctgttcc ggcgcatctt
      421 cgccgagggg gcgttcaccg ccgccttcgt gccgacctac gccaaggtgc tggcggccga
      481 ggggcaggag gcggcggaca atctcgcgac cgacgccctg gcgaccctgg cggcgatcac
      541 cctggttctg accgtcgcct tccagctggc gatgccgtgg ctgatgctgg cgatcaaccc
      601 ggggttcggc ttcaacagcg agaagtacag gctggcggtg gtgctgaccc agatcagcat
      661 gccctacctg ccctgcatgg ccatcgtcgc cctgctgtcc ggagtgctca acgccggcgg
      721 ccggttcgcc ctgtcggccg ccgcgccgac ggtgctcaac gcggtgatcc tgatcgcggt
      781 gattccgccg cgcgacgcca tcggctcggc ctatgcggca acctgggcga tcttcgccgc
      841 cggcctggtc caggcgggcc tgctgtactg gggcgcgcga cgcacggggg cgaagatccg
      901 gccgacgatc ccgcgcctga cgcccgagat cagggccctg atcgccgtgg ccatcccggg
      961 cgcggtggcc gccagcgtca cccagatcaa catcttcctg tccggcatca tcgccagcca
     1021 ggtcaacggc gcccgcacct ggctgtcggt cgccgaccgg ttctaccagc tgccgctggg
     1081 cctggtcggg gtggccatcg gcgtggccct gctgccgtcg ctgtcgcggg ccatcggcct
     1141 gggcgacaag gtcgaggcgc agaggaccac cgaccaggcg atcgtcttcg ccatggccct
     1201 gaccctgccg gcggcggccg tgctcatggc catgccgcac tacctgatcg acggcctgtt
     1261 ccagcgcggc gagttcagcc cctacgacgc cagcgagacg gccaaggccc tgttccacta
     1321 cggctggggc gtgccggcct tcgtgctggc gcgcgtgctg tcgccggtgt tcttcgcgcg
     1381 gcacgacacc cgggcgccga tgcgcttcgc cctgatctcg gtcggggtca acatcggcct
     1441 gggcctggtc ctgttctaca ccatcggctt cgagggcatc gccgcggcga cggccttcgc
     1501 ctcctggctc aacgtcgccc agatggtcca tgccctgcgt cggcgtggcg actggacccc
     1561 gtcaccggcc gcctggagca aactggcccg catcgccatg gccagcggcc tgctgggcgc
     1621 ggccatgttc gccgcccagc tgttccgggc cggccttgaa gcgcccctcg ccggcgtcag
     1681 ggtcattggc ctgggcccca aggaaatcac cgtcctggcg ctctgcctgg ccggcgcggc
     1741 cctttatccg ctgcttctgc tcggcagcgg cggcgtcacc ctggccgaga tccgcgaagc
     1801 cctgcggcgc aagcccgggg ccgccgccgc gccgccgtcg gacttgccct gatcgccgcc
     1861 ctgccgtatc tggccgtcat ggtcctcgcg gcacataccc cgcgccgatg gcgcagcgtc
     1921 tgatcgacac ccgctgagct tcgcgcggcg acctgtccgc gcgtccatcg acctgacatt
     1981 caagaagacc gatccatgac cgatcagcct cccgccccct atgccggacc gcaacgcgtg
     2041 ctgtccggcg tccagccgtc cggcgccctg cacctgggca actatctcgg cgccctggtg
     2101 aagttcgccc ggctgcagca cgaacaggag tgcctgttct gcgtcgtcga cctgcacgcc
     2161 atcacggtgt ggcaggaccc ggcccagctg acggcccaga cgcgcgagat cgccgcggcc
     2221 tacatcgccg ccggggtcga tccggcgaag gccggcatct tcccgcagtc ggccgtcggc
     2281 gcccatgccg agctggcctg gatcctccag tgcgtcgccc ggctcggctg gctcgaccgg
     2341 atgacccagt tcaaggagaa gagcggcaag cacaaggagc gcagctcggt cggcctctac
     2401 acctatccgg tgctccaggc ggccgacatc ctggtctaca aggcgaccca tgtgccggtc
     2461 ggcgaggatc agaagcagca cctcgaactg acccgcgaca tcgccgccaa gttcaaccac
     2521 gacttcgacc ggccgggctt cttcccgctg cccgacgggc tgatcgaggg gccgggcgcg
     2581 cggatcatga gccttcgcga cgggacggcg aagatgagca agtccgaccc gtcggacaac
     2641 tcgcgcatca acctgaccga cgacgccgac gccatcgccc agaagatccg caaggccaag
     2701 accgatcccg agcccctgcc cgagtcgctg gacgacctga aggcgcggcc ggaagccgac
     2761 aatctcgtcg gcatctacgg ggcgctggcc gggttgaccc gggcccaggt gctggccgag
     2821 ttcggcggca agggatttgg cgtcttcaaa ccggccctgg ccgaccttgc ggtggagaaa
     2881 ctcggtccgg tcagcgaccg catgcgccgg ctgatggccg acccggccga gatcgaccgc
     2941 gtgctggcgg ccggggcgga caaggcccgc gaggtggccg cccccacgct ggacgaggtc
     3001 aagaggatcg tcggcttctg gcggtaatca ccttgcgtcc ttcgacacgc gcttcgcgct
     3061 gctcaggatg acgtgcattt gtatacgtca tggcgagcag cgcccgaagg gtgcgtgtcg
     3121 aaccacgcaa tgacggagcc gcttgctctc gaccgcatcg tcagccaccg tcgctccgat
     3181 gagccagcgc aagttcctcg tcatcgccga tgacagcccg gagtcggggg cggcgctgcg
     3241 ctacgcctgc cggcgggcgc ggtcgaccgg cggacacgtc tgcctgctgc gggtcatcga
     3301 gccggcggtg ttcgaacact ggtcgggcgt gcgcgaggag atggagcggc aggagcgggt
     3361 cgaggccgag atggtcctgc agaagctggc cgagacggtc gtggccgtca ccggcaaggc
     3421 ccccgagttc ctgatcaagc atgccgagaa caccaagtcg gcgatccggt cggtggtggc
     3481 caatgacccc gacatcaaga tcctcgtcct ggccgcctcg accggcggca agggcccggg
     3541 cccgctggtc gcctcgctgg cgcgggacgg cgtcaactgg ggcgcgcgca agctgccggt
     3601 caccgtggtg ccgggcgatc tgaccgacga ggagatcgcg gacctggcat gagcggggaa
     3661 ccgggcttca ccccaagttc gccgctgacc cgcaagccgc gcaaacgaag ctggctgttc
     3721 gcctgggtcc tgctcgcgct cgtggccggc atgaccctga tcgtcctggc cggtccgcgc
     3781 gtgtccccgg ccgcgtggcg ggccgcgggc gcggcctacg gcgtcctgct ggccttcatg
     3841 gccgtcagac aggtctggct catccggcgg gacctgcgat cgcggcgtca tcgcgacc
//
LOCUS       DAORNX010000286         3895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17472_length_3895_cov_7.661198, whole genome shotgun sequence.
ACCESSION   DAORNX010000286 DAORNX010000000
VERSION     DAORNX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17472_length_3895_cov_7.661198"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLO65_11935"
     CDS             complement(<1..235)
                     /locus_tag="PLO65_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRD29000.1"
                     /translation="MNLFGRSTFSHGDRIVAHGQPVRLSVNARARRISLRVDRVRREA
                     IATAPSARRLPEAVAFARERADWIADQLADLPAV"
     gene            complement(279..1529)
                     /gene="phaZ"
                     /locus_tag="PLO65_11940"
     CDS             complement(279..1529)
                     /gene="phaZ"
                     /locus_tag="PLO65_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRD29001.1"
                     /translation="MLYALHEATYYAASPMRAAARATRDWWSSPLNPASNTSFGRKLS
                     AGADLYANVTRRYGKPDWMIDEITVNDHRVRVRPTVVWESPWCRLVQFDRDMADMRRA
                     GKLQLDPAVLIVAPMSGHYATLLRGTVEAFAPDHAVFITDWSNARDVPMMEGRFDFDD
                     YIDHIRMMLEALGHRAHVVAVCQPGPPVLAAAALMAEDENPYRPRTMTFMGSPIDARL
                     SPTVTNRLAEERPFTWFQSNMIYTVPPPYPGVGRRVYPGFVQLASFMSMNMERHQEAH
                     RRYFDHLVSGDGDSADKHRAFYDEYLAVMDLTEEFYLQTIDKVFQSYALPKGELTHRG
                     RLVRPAEISDIGLMTVEGELDDISGIGQTQAAHDLCPNIPADMKLDHVQQGVGHYGVF
                     NGRRFREEIFPRVQAFIRQCDPDF"
     gene            complement(1601..2194)
                     /locus_tag="PLO65_11945"
     CDS             complement(1601..2194)
                     /locus_tag="PLO65_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRD29002.1"
                     /translation="MSRQRLLLIAACALGLVLLGVIALGAGLFRPAPSAALIGGPFQM
                     VNQDGKPVDESLLKGKWSVVFFGYTACPDICPGTMQALRGAVDLLGPKGKDVQIVFVS
                     VDAGRDTPVRLRDWLDVQSLPPGTVGLTGTPQQVAAMARTYRVVYEIKGEGTDYLVNH
                     STAAYLMTPRGRFDRVLAYGLTPEQMADQIRRAMRGD"
     gene            2248..3687
                     /locus_tag="PLO65_11950"
     CDS             2248..3687
                     /locus_tag="PLO65_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HRD29003.1"
                     /translation="MTQTPSPAGLIQEEPGLDRPGRPAAQGWDALWPAGVPRRGRLRV
                     RTLTNLRWVAVAGQTVTVAVVAYGLKLPTPVAACFAVIALSAWLNVLVTLAASGQRLT
                     TDNEAAAHIAFDILQLSGLLYLTGGILNPFSLLLIGPATLAAATLPLRHAVLLASMAI
                     AASVFLAVTALPLPSPFAEPYAPPLLNRVGAVLARILGIISTAAYAWYAAQEAARMEL
                     ALDTAHTVLAREQRLSALGALAAAAAHELGTPLATISIVAKEMARNTEGSLREDADLL
                     ISQAERCRDILRRLTRAPEAADAVHERMTLLQVVQESIAPHARRPDVRVEAVVSGPHG
                     ETAPDIWRLAEVLHALTAFVENAVDFARSEILVTARFDARSIAIEVRDDGPGFAPDIL
                     VKLGEPYVTSRPGAEAMRSGHVGMGLGFFIAKTLLERTGATVDFRNGRQGGAIITARW
                     RREAIEAPVDHDAFSQNSGLEDPAYAATQ"
     gene            3731..>3895
                     /locus_tag="PLO65_11955"
     CDS             3731..>3895
                     /locus_tag="PLO65_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HRD29004.1"
                     /translation="MTDLATLIAALEDRSLLVLDDDAALRTRLGRALEQRGFEVRTAG
                     GVTEALEAVRA"
BASE COUNT          576 a         1327 c         1380 g          612 t
ORIGIN      
        1 tgacggcggg caggtcggcc aactggtcgg cgatccagtc ggcccgctcg cgggcgaagg
       61 ccaccgcctc gggcagccgg cgggcgctgg gcgctgtggc gatcgcctcg cgcctgaccc
      121 ggtcgaccct caggctgatc cggcgggcgc gcgcgttgac cgacaggcgc accggctggc
      181 cgtgcgccac gatgcgatcg ccatggctga aggtggaacg gccgaacaga ttcatcaacc
      241 cgtgcttagg ccgggccgcg cgtcacgaaa agcccgtttc agaaatcggg atcgcactgg
      301 cggatgaagg cctgcacccg gggaaagatc tcctcgcgga agcgacggcc gttgaacacg
      361 ccgtagtggc cgacgccctg ctggacatgg tccagcttca tgtcggccgg aatgttcggg
      421 cacaggtcgt gggccgcctg ggtctggccg atgccggaga tgtcgtccag ttcgccctcg
      481 accgtcatca ggccgatgtc ggagatttcc gcgggccgca ccagccgccc gcgatgggtc
      541 agctcgccct tcggcagggc gtaggactgg aacaccttgt cgatggtctg gaggtagaat
      601 tcctcggtca gatccatcac cgccaggtat tcgtcataga aggcgcggtg cttgtcggcg
      661 ctgtcgccgt cgcccgagac cagatggtcg aagtagcgac ggtgggcctc ctggtgtcgc
      721 tccatgttca tcgacatgaa gctggccagc tgcacgaagc ccggatagac gcgccggccg
      781 acgcccggat aaggcggcgg cacggtgtag atcatgttcg actggaacca ggtgaacggc
      841 cgttcctcgg ccagccggtt ggtcacggtc ggggacaggc gcgcgtcgat cggcgaaccc
      901 atgaaggtca tggtccgggg ccggtagggg ttttcgtcct cggccatcag ggccgccgcg
      961 gcgagcaccg gcgggccggg ctggcagacg gcgaccacgt gcgcccggtg gccgagggct
     1021 tcgagcatca tccggatgtg gtcgatgtag tcgtcgaagt cgaaccggcc ctccatcatc
     1081 ggcacgtcgc gggcgttcga ccagtcggtg atgaacaccg cgtggtcggg cgcgaaggcc
     1141 tccacggtgc cgcgcagcag ggtcgcgtag tggcccgaca tcggcgcgac gatcagcacc
     1201 gccggatcga gctggagctt gccggcccgg cgcatgtcgg ccatgtcgcg gtcgaactgg
     1261 acgagccggc accaggggct ttcccagacc acggtcgggc ggacccggac cctgtgatcg
     1321 ttgacggtga tctcgtcgat catccagtcg ggcttgccgt agcggcgcgt gacgttggcg
     1381 tacaggtcgg cgccggccga cagcttgcgg ccgaacgacg tgttgctggc gggattgagc
     1441 ggcgaagacc accagtcgcg ggtcgcgcgc gcggcggcgc gcattggcga tgcggcgtag
     1501 taggttgcct cgtgcagggc gtagagcatc gaacctcgtt cgttgtgcag cgcagcatag
     1561 cacggcgcgc cttaacgaag tccagtccct tttggagggc ttagtcgccg cgcatggccc
     1621 ggcggatctg gtccgccatc tgttcgggcg tcaggccgta ggcgagcacc cggtcgaacc
     1681 ggccgcgcgg cgtcatcagg taggcggccg tcgagtgatt gaccaggtag tcggtcccct
     1741 cacccttgat ttcgtagacc acgcgatagg ttcgggccat ggcggccacc tgctgcggcg
     1801 tgcccgtcag gccgacggtc ccgggcggca ggctctggac atccagccag tcgcgcagcc
     1861 ggaccggcgt gtcgcgaccg gcgtcgaccg aaacgaacac gatctgcaca tccttgcctt
     1921 tcggaccgag cagatcgacg gcgccgcgca gcgcctgcat ggtgcctggg cagatgtcgg
     1981 ggcaggcggt gtagccgaag aagaccacgc tccacttgcc cttgagcagg ctctcgtcga
     2041 ccggcttgcc gtcctggttg accatctgga acgggccgcc gatgagggcc gccgacggcg
     2101 ccggccggaa caggcccgcg cccagggcga tgactccgag caggaccagg cccagggcgc
     2161 aggcggcgat cagcaacaga cgttgacgcg acaaggattt ctccatcggg gctcgccttg
     2221 ggccgctaac gggaccactc tggcgacatg acgcagaccc cgtcaccggc cggcttgatc
     2281 caggaggagc ccggcctcga taggcccggt cgcccggcgg cgcaagggtg ggacgccctc
     2341 tggccggccg gcgtgccgcg tcgcgggcgg ctgcgggtcc ggaccctgac aaaccttcgc
     2401 tgggtcgcgg tggccggaca gaccgtcacc gtggcggtcg tggcctatgg cctgaagctg
     2461 ccgacgcccg tggccgcctg cttcgcggtc atcgccctgt cggcctggct caacgtgctg
     2521 gtgaccctcg ccgcctccgg gcagcggctg accacggaca atgaggcggc cgcccatatc
     2581 gccttcgaca tcctccagct gtcgggcctg ctctatctga cgggcggcat cctcaatccc
     2641 ttctccctgc tgctgatcgg cccggcgacc ctggcggcgg cgaccctgcc gcttcgccat
     2701 gccgtcctgc tggcctccat ggcgatcgcc gccagcgtct tcctcgccgt cacggccctg
     2761 cccctgccgt caccgttcgc cgaaccctat gcgccgccgc tgctcaaccg ggtcggcgcg
     2821 gtgctggcgc ggatcctggg catcatctcc accgccgcct acgcctggta tgcggcgcag
     2881 gaggcggcgc ggatggaact ggcgctcgac accgcgcaca ccgtgctggc gcgggaacag
     2941 cggctgtcgg ccctgggcgc gctggcggcc gccgcggcgc acgagctggg cacgccgctg
     3001 gcgacgatct cgatcgtggc caaggagatg gcccgcaata ccgagggatc gctacgcgag
     3061 gacgccgacc tgctgatctc ccaggcggag cgctgtcgcg acatcctgcg gcggctgacc
     3121 cgggcgcccg aggcggccga cgcggtgcac gagcgcatga ccctgctgca ggtggtccag
     3181 gaatcgatcg cgccgcacgc ccgtcggccc gatgtgcgcg tcgaggcggt cgtgtctggc
     3241 ccgcatggcg agacagctcc cgacatctgg cggctggcgg aggtgcttca cgccctgacg
     3301 gcctttgtcg agaacgcggt cgacttcgcc cggtcggaaa ttctggtgac ggcccgcttc
     3361 gatgcgcgct ccatcgcgat cgaggtgcgc gacgacgggc cgggtttcgc ccccgacatc
     3421 ctggtcaagc tgggcgagcc ctatgtcacc agccgcccgg gcgcggaggc gatgcgcagc
     3481 ggccatgtcg gcatgggcct ggggttcttt atcgccaaga cgctccttga gcggaccggc
     3541 gcgacggtgg atttccgcaa cgggcggcag ggcggcgcga tcatcaccgc gcgctggcgc
     3601 cgcgaggcga tcgaggcgcc agtcgatcac gacgccttct cgcagaattc aggacttgaa
     3661 gaccccgctt acgctgcgac acaatgacgc gaatttcaac tgccgcgcga acgacgacga
     3721 ggacgttttg gtgaccgatc ttgcaaccct catcgccgcc cttgaagacc gcagcctgct
     3781 ggtgctcgac gatgacgcgg cgcttcgcac ccgcctggga cgggccctgg agcagcgcgg
     3841 cttcgaggtc cgcacggccg gcggcgtcac cgaggccctc gaggccgtcc gggcc
//
LOCUS       DAORNX010000287         3888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17518_length_3888_cov_6.402296, whole genome shotgun sequence.
ACCESSION   DAORNX010000287 DAORNX010000000
VERSION     DAORNX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17518_length_3888_cov_6.402296"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLO65_11960"
     CDS             complement(<1..764)
                     /locus_tag="PLO65_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRD29005.1"
                     /translation="MPSAAARRQYSGPALFSFGFRPFFLGAALWAVLAVPLWLWVHLG
                     GPAGATPVDRDWHVHEMLFGYVGGVIAGFLLTAVPNWTGRLPVTGAPLVLLFGLWLAG
                     RVAMLVATVPPAAAMVDAAFLVVFAGVVWREVLAGRNWRNLPVAVMVSLLALANLTFH
                     ARLLWPDLGPPAERGAIAMIALLIAFIGGRVVPSFTRNWMMQARLTPLPAAEDRLDRL
                     GVIVAAAGLAAWIAFPDHPLAGVGLCLGGAATLARLA"
     gene            complement(873..3104)
                     /locus_tag="PLO65_11965"
     CDS             complement(873..3104)
                     /locus_tag="PLO65_11965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRD29006.1"
                     /translation="MAFDTALAGEIWSSKYRFTPQGGDAESAVEDTWRRVARAVARAE
                     DRELREPVEAEFYDALRDHRFLPAGRIIAGAGTARTVTLFNCFVMGRIPDSLGGIFEH
                     VREAALTLQQGGGVGMDFSSIRPAGAFVSGVAADASGPLSFMDVWDAMCRTIMSAGQR
                     RGAMMGCLRIDHPDIEAFIDAKREPGRLRNFNLSVLVSDAFMAALQAGADWPLTFGGR
                     VHRVLPAAELWARLMRATYDCAEPGVIFIDRVNAANNLAHCEEILASNPCGEQMLPAY
                     GACLLGSINLARLVRTPFEAGATIDEDELGRLTHTAVRLLDNVIDISRFPLEAQAAEA
                     VSKRRLGLGVTGLANALAFCGQRYGDPAAADLTRRWLGVIKREAYRTSARLAAEKGVF
                     PLYDASMLDRPNLASLDDETRELIRAHGLRNGCLTSIAPTGTTSILAGNVSSGIEPVF
                     AYAYGRKVRQPDGTTREEPVEDYALTVWKALHGGAPPPPELFVSAQSLTPADHLRMQA
                     AAQTMVDSSISKTVNCPEDIVFDDFSGIYVEGYRLGCKGLTTYRPNAITGSVLSVAAP
                     AAPPPPRVAEPQLTPRPAALDGMTYKIKWPESDHAVYVTLNDTVDGDGRRPFEIFVNS
                     RNMDHYAWTVGLTRMISAVFRRGGDVSFVAEELKAVFDPRGGAWLDGRYVPSLLAAIG
                     GVVEQHMTGLAPVLSDEDSVMAASPESTAKRLACCPRCGAAALVRKEGCDTCLDCGHS
                     KCG"
     gene            3294..3806
                     /locus_tag="PLO65_11970"
     CDS             3294..3806
                     /locus_tag="PLO65_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRD29007.1"
                     /translation="MPLEALAAPARTLEEAWAEAGPAALIDHIIRRYHDVHRDQLPAA
                     IAMARRVEATHYSHPRCPRGLADHLAVMTDHLLSHQYREEAVLFPMMLSPEHPMVRFP
                     IARMTEEHRDIDEQLLRLAVLTTDFSPPDDACATWRALYRACHELERDLREHVWLENE
                     VLFPAFLAAG"
BASE COUNT          631 a         1372 c         1378 g          507 t
ORIGIN      
        1 gccagccgcg ccagagtcgc ggccccgccg aggcagagcc cgacgccggc gaggggatgg
       61 tcggggaagg cgatccaggc cgccaggccg gcggccgcga cgatcacccc gaggcggtcg
      121 agacggtcct cggccgccgg caggggcgtc agcctggcct gcatcatcca gttgcgcgtg
      181 aagctcggaa cgacccgacc gccgatgaag gcgatcagca gggcgatcat cgcgatcgcg
      241 ccgcgctcgg ccggcggacc caggtccggc cacagcagac gggcgtgaaa ggtcaggttg
      301 gccagggcca gcaggctgac catcaccgcc accggcaggt tgcgccagtt gcggccggcc
      361 agcacctcac gccagaccac gcccgcgaac accaccaaga aggcggcgtc gaccatggcg
      421 gcggccggcg ggacggtcgc cacgagcatc gccacgcgtc cggccagcca aaggccgaac
      481 agcaacacca gcggcgcgcc ggtcaccggc agacggcccg tccagttggg cacggcggtc
      541 agcaggaagc cggcgatgac cccgccgacg tagccgaaca gcatctcatg cacatgccag
      601 tcgcgatcga ccggcgtcgc gccggccggg ccgcccagat ggacccacag ccatagcggc
      661 acggccagca cggcccacag cgcggcgccg agaaagaacg gccggaagcc gaagctgaac
      721 agcgccggcc cggaatactg gcggcgggcg gcggcggacg gcatgacggc gactcacagg
      781 agcgggatcg gacccctccc cttaggccct ccggagccgg cccgccttga gccgcgtgcg
      841 cccgcgactt gtcgtcgatc aagcgggcgg tcctagccgc atttcgagtg gccgcaatcg
      901 agacaggtgt cgcagccctc cttgcgcacc agcgccgccg cgccacagcg cggacagcag
      961 gcgaggcgtt tggccgtaga ctccggcgac gcggccatca cactgtcctc gtcgctcaga
     1021 accggcgcca ggccggtcat atgctgctcg acgaccccgc cgatggccgc cagcagcgag
     1081 gggacatagc gcccgtcgag ccaggccccg ccgcgcggat cgaacaccgc cttgagctcc
     1141 tcggccacga acgacacgtc tccgccccgg cggaacacgg ccgagatcat ccgcgtcagg
     1201 cccacggtcc aggcgtagtg gtccatgttg cgcgagttga cgaagatctc gaacggccgc
     1261 cggccatcgc cgtcaaccgt gtcgttgagc gtgacgtaga cggcgtggtc gctctccggc
     1321 catttgatct tgtaggtcat gccgtcgagg gcggccgggc ggggcgtcag ctgcggttcc
     1381 gcgaccctcg gcggcggcgg ggcggccggc gcggcgacgc tgagcaccga gcctgtgatc
     1441 gcgttcggtc ggtaggtcgt caggcccttg cagcccagcc ggtagccttc gacgtagatc
     1501 cccgagaagt cgtcgaaaac gatatcctcg gggcagttga ccgtcttgga gatcgagctg
     1561 tcgaccatgg tctgggccgc cgcctgcatg cgcaggtgat cggccggcgt cagggactgg
     1621 gcgctgacga acagctccgg cggcggtggg gccccgccgt gcagcgcctt ccacaccgtc
     1681 agggcgtagt cctcgaccgg ctcctcgcgc gtcgtaccat ccggctggcg gaccttgcgg
     1741 ccgtaggcat aggcgaacac cggctcgatg ccggacgaga cgttgccggc gaggatcgag
     1801 gtcgtgccgg tcggggcgat cgaggtcagg cagccgttgc gcaggccgtg cgcgcgaatc
     1861 agctcacggg tctcgtcatc gagcgaggcc aggttgggcc ggtcgagcat ggaggcgtca
     1921 tagagcggga agacgccctt ctcggccgcc agccgggcgg aggtgcgata ggcctcgcgc
     1981 ttgatcacgc caagccatcg ccgggtcagg tcggccgccg ccgggtcgcc gtaacgctgg
     2041 ccgcagaagg ccagggcgtt ggccaggccg gtgacgccaa ggcccaggcg gcgcttggac
     2101 accgcttcgg ccgcctgcgc ctcgagcgga aaacgggaga tgtcgatgac gttgtcgagc
     2161 agccgcacgg cggtgtgggt caatcggccc agttcgtcct cgtcgatcgt cgcgccggcc
     2221 tcgaacgggg tcctgaccag ccgggccagg ttgatcgagc ccagcaggca ggcgccgtag
     2281 gcgggcagca tctgctcgcc gcacggattg ctggccagga tctcctcgca atgggcgagg
     2341 ttgttggccg cgttgacccg atcgatgaag atcacccccg gctcggcgca gtcgtaggtc
     2401 gcccgcatca gccgcgccca cagttcggcg gcgggcagca cccggtgcac gcgcccgccg
     2461 aaggtcagcg gccagtccgc gccggcctgc agcgcggcca tgaaggcgtc gctgaccagc
     2521 accgaaaggt tgaagttgcg cagccgcccg ggctcccgct tggcgtcgat gaaggcctcg
     2581 atgtcgggat ggtcgatgcg caggcagccc atcatcgcgc cgcgccgctg tccggccgac
     2641 atgatggtgc ggcacatggc gtcccagacg tccatgaagc tgagcgggcc cgaggcgtcg
     2701 gccgcgacgc cggagacgaa cgctccggcc ggccggatcg aggagaagtc catgcccacg
     2761 ccgccgccct gctgcagggt cagggcggcc tcccggacat gctcgaagat gccgcccaga
     2821 ctgtcgggta tccggcccat gacgaaacag ttgaacaggg tcaccgtccg cgccgttccg
     2881 gccccggcga tgatgcggcc agccggcagg aagcgatgat cgcgcagcgc gtcatagaat
     2941 tccgcctcga ccggttcacg aagctcgcga tcctcggcgc gcgccacggc ccgggccacc
     3001 cgtcgccagg tgtcctcgac ggcggactcg gcgtctccgc cctggggggt gaatcgatat
     3061 ttgctggacc agatctcgcc ggccagggcc gtgtcgaacg ccatgacgaa cctcgtgagg
     3121 gagaaggggc ggcaggctcc ccgactcgct gcggcgcgcg aaagggcgct ggccctgtcg
     3181 cagcggcccc ggatcggcaa gccgcgcctt gccaatggtc aagcggcgac gccgcccgct
     3241 tcaaggcgcg tggccgcgcg ccggactaag ctgggagcaa aggagaccgc gacatgcccc
     3301 tcgaagccct cgccgcgccg gcccgcaccc tggaggaagc ctgggccgaa gccggcccgg
     3361 cggcgctgat cgaccacatc atccggcgat atcacgacgt ccaccgtgac cagcttccgg
     3421 cggccatcgc catggcccgg cgcgtcgagg cgacgcacta cagtcatcct cgctgcccgc
     3481 gcgggctcgc ggaccatctg gcggtcatga ccgaccacct cctgtcgcat cagtaccggg
     3541 aggaagcggt gctgtttccg atgatgctgt cgccagagca tccgatggtc cgcttcccga
     3601 tcgcccggat gaccgaggaa catcgcgaca tcgacgaaca gctgttgcgg ctggcggtgc
     3661 tgacgaccga tttctcgccg ccggacgacg cctgcgcgac ctggcgggcc ctctaccgag
     3721 cctgtcacga acttgaacgc gacctgcgcg aacatgtgtg gctggagaac gaggtcctgt
     3781 tcccggcgtt cctggcggcc ggctagaggt cgaggctgtc gaccagatcg cccgcggcgg
     3841 ccggcgccgc gccaggacga cggcgaagca gcaggtccga ggcggcca
//
LOCUS       DAORNX010000288         3886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17535_length_3886_cov_7.832420, whole genome shotgun sequence.
ACCESSION   DAORNX010000288 DAORNX010000000
VERSION     DAORNX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17535_length_3886_cov_7.832420"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLO65_11975"
     CDS             complement(<1..465)
                     /locus_tag="PLO65_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29008.1"
                     /translation="MALSGMTGFARVEGAEGAWSWAVEARSVNGRNLEVRFRGPPGFD
                     LLEKGVRDGAQARFQRGQINVGLQARRSESPVAVSVNHDQLDRYLATVDPLVRSGRVQ
                     PPTADGLLALRGVLEAVDAEDDPQVRAAVEQAMASTLLAALDALRASRLAEGA"
     gene            complement(466..1434)
                     /gene="mltG"
                     /locus_tag="PLO65_11980"
     CDS             complement(466..1434)
                     /gene="mltG"
                     /locus_tag="PLO65_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRD29009.1"
                     /translation="MASSAFATLFIALVVFGLWAVWSYRGPGPETRDGRPATVMLRKG
                     AGLNEIAASLEGARIVRSGSIFLAAAQLTGAARSLKAGEYEFPSGAPMSRVLDDIRAG
                     RIVRHQVTIPEGTTSEMAVEILNRESALTGVAPTPPEGAILPETYDFQRGEDRAAVLQ
                     RMMDARDRLLATLWSKRKAGLPISTPEEAVILASIVEKETGVASERPQVASIFVNRLR
                     KGMRLESDPTIIYGITKGRPLGRRILLSELKAPTPYNTYAIEGLPPTPIANPGRASLA
                     AVLDPPETDYLFFVADGTGGHAFAATYEEHQRNVEKWRAWRARTGR"
     gene            complement(1488..2768)
                     /gene="fabF"
                     /locus_tag="PLO65_11985"
     CDS             complement(1488..2768)
                     /gene="fabF"
                     /locus_tag="PLO65_11985"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRD29010.1"
                     /translation="MRRVVVTGLGLLTPLGHGVEATWKGILAGKSGAGRITTFDPEDY
                     ACQVACEIPRVDGRGGGGPDVAGSFNPDDTMAPKDQRKVDDFILYAMAAADEAVRDAG
                     LTEVDEETALRTGVIIGSGIGGLATIARTALELAEKGPRRISPFFIPSALINLASGQV
                     SIRYGFKGPNHSVVTACATGAHAIGDATRLIKYGDADIMIAGGAEASICPIGVAGFIA
                     CRAMATDFNDRPESASRPYDKDRGGFVMGEGAGILVLEEYEHARARGAKIYAEVVGYG
                     LSGDAYHITAPSEDGDGGFRAMTAALKDSGLQASDIDYVNAHGTSTMADGLELGAVTR
                     LLGDHAKNVAMSSTKSMIGHLLGAAGAVEGIFSILALRDQIAPPTINLDNPEFDTLID
                     LVPHKAKPMAIDVAMSNSFGFGGTNACVIFKKAP"
     gene            complement(2885..3124)
                     /locus_tag="PLO65_11990"
     CDS             complement(2885..3124)
                     /locus_tag="PLO65_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRD29011.1"
                     /translation="MSDILERVRKIVIEHLDADPEKVTEKASFIDDLGADSLDNVELV
                     MAFEEEFDIEIPDDAAEHIQTVGDAVKFISEKVGG"
     gene            complement(3247..>3886)
                     /gene="fabG"
                     /locus_tag="PLO65_11995"
     CDS             complement(3247..>3886)
                     /gene="fabG"
                     /locus_tag="PLO65_11995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRD29012.1"
                     /translation="LSGTRETALEALAAELGGRTSAVTANLSDPASVDGLLAAAEAAS
                     GLPIDILVANAGITRDGLMLRMKDEDWEQVIKVNLESYFRLSRAALKGMMKRRWGRII
                     GITSVVGVTGNPGQANYAASKAGMIGFSKALAQEVASRNVTVNTVAPGFIASPMTDGL
                     NEAQREAILGKIPAGRLGSGEDVAAACVYLASDEGAYVTGQTLHVNGGMAMI"
BASE COUNT          572 a         1307 c         1372 g          635 t
ORIGIN      
        1 ggcgccctcg gccagtcgcg aggccctcag ggcgtcgagc gcggccagca gggtgctcgc
       61 catggcctgt tcgaccgccg cgcggacctg cgggtcgtcc tcggcgtcga cggcctccag
      121 cacgccgcgc agggccagca ggccgtcggc cgtcggcggc tggacgcgcc ccgaccggac
      181 cagcggatcg accgtggcga ggtaacggtc cagctggtcg tgattgaccg acacggcgac
      241 cggtgattcc gaacgccggg cctgcaggcc gacgttgatc tggccgcgct gaaaccgggc
      301 ctgggccccg tcgcgcacgc ccttctcgag caggtcgaag ccgggcgggc cgcggaagcg
      361 aacctcgaga ttgcggccgt tgaccgagcg ggcctcgacc gcccagctcc atgcgccctc
      421 ggcgccctcg acccgggcga agccggtcat gccggacagc gccatctatc tgcccgtccg
      481 cgcgcgccag gcgcgccatt tctcgacgtt gcgctggtgc tcctcgtagg tcgcggcgaa
      541 ggcatggccg cccgtgccgt cggcgacgaa gaacaggtag tcggtctcgg gcgggtccag
      601 cacggcggcc agcgacgccc gccccggatt ggcgatcggc gtcggcggca ggccctcgat
      661 ggcgtaggtg ttgtagggtg tgggcgcctt cagttcggac agcaggatgc gtcgtccgag
      721 cggccgcccc ttggtgatgc cgtagatgat cgtcggatcg ctctccagcc gcatcccctt
      781 gcgcaggcgg ttgacgaaga tggaggctac ctgcggccgt tcactggcga cgccggtttc
      841 cttctcgacg atcgaggcaa ggatgaccgc ctcctcgggc gtactgatcg gcaggcccgc
      901 cttgcgcttc gaccagaggg tggcgagcag cctgtcgcgg gcgtccatca tccgctggag
      961 caccgcggcc cggtcctcgc cccgctgaaa atcgtaggtc tcgggcagga ttgcgccctc
     1021 gggcggggtc ggggccacgc cggtgagagc cgattcgcga ttgagaatct cgacggccat
     1081 ctccgaggtg gtcccctcgg gaatggtcac ctggtgacgc acgatgcggc ccgcgcggat
     1141 gtcgtccagc acccgcgaca tcggggcgcc ggaaggaaac tcgtattcgc cggccttgag
     1201 actgcgcgcg gcaccggtca gctgggcggc ggcgagaaag atcgaacccg accggacgat
     1261 cctggcgccc tcaagggagg ccgcgatctc gttcagcccc gcgcccttgc gcagcatgac
     1321 cgtggccggt cgcccgtcgc gcgtctcggg acccggacca cggtagctcc acaccgccca
     1381 cagcccgaac acgaccaggg cgatgaacag ggtcgcgaac gcgctggagg ccatgatcag
     1441 caggcggcgc atcagcggca ggccctggga aggggcggtt ttccggctca cggggccttc
     1501 ttgaagatga cacaggcgtt cgtgccgccg aagccgaagc tgttggacat cgccacgtcg
     1561 atcgccatcg gtttcgcctt gtgcgggaca aggtcgatca gggtgtcgaa ctccggattg
     1621 tcgagattga tcgtcggcgg cgcgatctgg tcgcggaggg ccaggatcga gaagataccc
     1681 tcgaccgcgc cggccgcgcc gagcaggtgg ccgatcatcg acttggtcga ggacatggcc
     1741 acgttcttcg cgtggtcgcc gagcaggcgg gtgaccgcgc cgagttccag gccgtcggcc
     1801 atggtcgagg tgccgtgggc gttgacgtag tcgatgtccg acgcctgcag gccggaatcc
     1861 ttcagcgccg ccgtcatggc gcggaagccg ccgtcgccgt cctcggacgg ggcggtgata
     1921 tggtaggcgt cgcccgacag gccgtagccg accacctcgg cgtagatctt cgcgccccgg
     1981 gccctggcgt gctcgtactc ctcgagcacc aggatgccgg cgccctcgcc catgacgaaa
     2041 ccgccgcgat ccttgtcata ggggcgcgag gccgactcgg gccggtcgtt gaagtcggtg
     2101 gccatggcgc ggcaggcgat gaagccggcg acgccgatcg gacagatcga ggcctcggcg
     2161 ccgccggcga tcatgatgtc ggcgtcgccg tacttgatca gtcgcgtcgc gtcgccgatg
     2221 gcgtgggccc ccgtggcgca ggcggtcacc accgaatggt tggggccctt gaagccgtag
     2281 cggatcgaga cctggccgga ggccagattg atcagggccg aggggatgaa gaacgggctg
     2341 atccggcgcg gccccttctc cgccagttcc agggcggtgc gggcgatggt cgccagaccg
     2401 ccgatgcccg agccgatgat cacgccggtg cgcagcgcgg tttcctcgtc cacttcggtc
     2461 aggccggcgt cgcgcacggc ctcgtcggcg gccgccatgg cgtagaggat gaagtcgtcg
     2521 accttgcgct ggtccttggg agccatggtg tcgtcgggat tgaatgaccc ggccacgtcc
     2581 ggcccgccgc cgccgcgacc gtcgacccgc gggatctcgc aggcgacctg gcaggcatag
     2641 tcctcggggt cgaacgtcgt gatgcggccc gcgccggact tgccggccag gatgcccttc
     2701 caggtcgcct cgacgccgtg ccccaggggg gtcaggaggc caaggccagt gacgacgaca
     2761 cgccgcatgg gatcaactcc ttcaccccgg caggtcccgg ggtcagctca aaacgaagcc
     2821 gccgcgcgcg tcgggttacc ccgacaccgc gcggcggtga atcgaccgga aacgggtctg
     2881 cggatcaacc gccgactttt tccgagatga acttcacggc gtcgccgacc gtctggatat
     2941 gttcggcggc gtcgtcggga atctcgatgt cgaattcttc ctcaaaggcc atcaccagct
     3001 cgacattgtc gaggctgtcg gcgccgaggt catcgatgaa gctcgccttc tcggtgacct
     3061 tttcaggatc ggcgtcgagg tgttcgatga cgatcttacg cacacgctcg agaatgtcgg
     3121 acattcgttc tggtcctctt taggtcttta gtggtccagc cgccgaagcg gtcgtggaaa
     3181 tcgggggctt cgctaccatg ttcccttcgg cgaggccagc ggccagccgt cggaccgtgg
     3241 cggttgtcag atcatcgcca tgccgccgtt cacgtgcaag gtctgaccgg tgacgtaggc
     3301 gccctcgtcg ctggccagat agacgcaggc cgcggccacg tcctcgccgg atccgagccg
     3361 gccggccggg attttcccaa gaatggcctc gcgttgcgcc tcgttgaggc catcggtcat
     3421 cggactggcg atgaagccgg gcgcgacggt gttcaccgtc acgttacggc tggctacttc
     3481 ctgggccagg gccttggaga agccgatcat cccggccttg gaggcggcgt agttggcctg
     3541 gcccgggttg cccgtgaccc cgaccaccga ggtgattccg atgatccggc cccagcgccg
     3601 cttcatcatg cccttcagcg ccgcccggga cagccggaaa taggattcca ggttgacctt
     3661 gatgacctgc tcccagtcct cgtccttcat ccgcagcatg aggccgtcgc gggtgatgcc
     3721 ggcgttggcc acgaggatgt cgatcggcag gcctgaggcg gcctcggcgg cggcgagcag
     3781 gccgtcgaca ctggccgggt cggacaggtt ggcggtcacc gcgctggtgc ggccgcccag
     3841 ctcggccgcc agggcctcga gcgccgtctc gcgtgtgccg gacagg
//
LOCUS       DAORNX010000289         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17571_length_3882_cov_6.135093, whole genome shotgun sequence.
ACCESSION   DAORNX010000289 DAORNX010000000
VERSION     DAORNX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17571_length_3882_cov_6.135093"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLO65_12000"
     CDS             complement(<1..533)
                     /locus_tag="PLO65_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRD29013.1"
                     /translation="MPSPDSHRGRVLIIAGSDSCGGAGIQADIKTVTALGGYAATAIT
                     AVTVQNTLGVTGVHPVPLDIIEAQARTVLDDIGADAIKTGMLGDVATVELVARVLDSA
                     AGVPAVIDPVMVAKGGASLLESRAVAAVKTLLVPRAALLTPNAPEAEALTGLTVATPD
                     DQRRAGDALLALGANAVL"
     gene            complement(685..>984)
                     /locus_tag="PLO65_12005"
     CDS             complement(685..>984)
                     /locus_tag="PLO65_12005"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRD29014.1"
                     /translation="PEARVILDPGVGFGKTLEHNLALLRALPRFVALGFPVLLGASRK
                     RFIAAIDPAAKDAGDRLGGSLAVALEGARAGVAAVRVHDVRETRQALAVQGAVGG"
     assembly_gap    985..1055
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1056..1455)
                     /gene="folP"
                     /locus_tag="PLO65_12010"
     CDS             complement(<1056..1455)
                     /gene="folP"
                     /locus_tag="PLO65_12010"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRD29015.1"
                     /translation="MGIVNVTPDSFSDGGRHLAPENALVHARKLVAQGADMLDIGGES
                     TRPGAAPVDEAEELARVIPLITAIRAESAVPISIDTLKPAVARAAVAAGATMWNDVSA
                     LRGPDAPATAAELGCEVVLMHMLGEPRTMQD"
     gene            complement(1678..3582)
                     /gene="ftsH"
                     /locus_tag="PLO65_12015"
     CDS             complement(1678..3582)
                     /gene="ftsH"
                     /locus_tag="PLO65_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRD29016.1"
                     /translation="MNLRNLAIWAVVVIIAAGVWSMVVQRGGAPGTTPAAEVHYSDLL
                     SQVDGGQIRSAVFRGATVDVVSQDKKAYTVVVPADSSDLVKRMEARGVNIAFKTGERS
                     LLLNILIQAFPILLLIAVWIFFMRQMQGGARGAMGFGKSKARLLTENKNRVTFEDVAG
                     VEEAKDDLTEIVDFLKDPAKFQRLGGKIPKGALLVGPPGTGKTLLARAIAGEAGVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEANEGIILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVTGREKI
                     LRVHMRNVPLAADVDVRTLARGTPGFSGADLANLVNEAALLAARKNRRMVTHDDFEMA
                     KDKVMMGAERRSMAMNEEEKKLTAYHEGGHALVAMSVPCADPVHKATIVPRGRALGMV
                     MQLPEGDRYSMKYAQMTSRLAIMMAGRVAEELIFGKENITSGASSDIKAATGLARAMV
                     TQWGYSDKLGTVAYGDNQEEVFLGHSVARTQNVSEETARLIDGEVKRLVQEGLDEARR
                     ILTERLDDLHKLAKALLEFETLSGEEIANVMKGVMPNRDEPEQKRPSGPSASVPLSPR
                     PGGVVARDAE"
     gene            complement(3655..>3882)
                     /locus_tag="PLO65_12020"
     CDS             complement(3655..>3882)
                     /locus_tag="PLO65_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HRD29017.1"
                     /translation="RVTALAGHAARLPEAERKRLSAIPAAARPALPAVIDRDGGVTCP
                     ILAERPTVRVRALALARFDAAMGRVDSEPAP"
BASE COUNT          533 a         1328 c         1358 g          592 t
ORIGIN      
        1 agcacggcgt tcgcgccgag ggccaacagg gcgtcaccgg cgcggcgctg gtcatcgggg
       61 gtggccaccg tcagcccggt cagggcctcg gcctccggcg cgttgggcgt caggagggcc
      121 gcgcgcggga ccagcagggt cttcaccgcc gccacggccc gcgactccag cagcgaggcg
      181 ccgcccttgg cgaccatcac cggatcgatg acggccggca cgcccgcggc gctgtccaga
      241 acccgggcga ccagttcgac cgtggccaca tcgccaagca tgccggtctt gatcgcgtcg
      301 gcgccgatgt cgtcgagaac ggtccgagcc tgggcctcga tgatgtccag cgggaccggg
      361 tgcacgccgg tgacgccgag ggtgttctgc acggtgacgg cggtgatcgc cgtggccgcg
      421 tagccgccca gcgccgtgac cgtcttgatg tccgcctgga tcccggcccc gccgcagctg
      481 tcggacccgg cgatgatcag gacgcggcca cgatgggaat caggggaggg catggggcgg
      541 ttgtagcggt ccctgcccgc gatgggcagg gtggttggtt cgacacgccc cggggcaact
      601 cgggctgctc gccatgacgt ctgcaatgca cgtcatcctg cgcgggcccg aagggccgtg
      661 tcctggaacg caccggcttc ccgtctagcc gcccaccgcc ccctgaaccg ccagcgcctg
      721 ccgggtctcc cgcacgtcgt gcacccgcac ggcggccaca cccgctctgg cgccttccag
      781 ggccacggcc agcgacccgc ccagccggtc gccggcatcc ttcgcggccg ggtcgatggc
      841 cgcgatgaac cgcttgcggc tggcgccgag caggaccggg aagcccagcg ccacgaaccg
      901 gggcagggcg cgcagcaggg cgaggttgtg ctcaagcgtc ttgccgaagc cgacgcccgg
      961 atcgaggatc acgcgcgcct cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtcc tgcatggtgc gcggctcgcc
     1081 cagcatgtgc atcagcacga cctcgcagcc gagttcggcg gccgtcgccg gggcatccgg
     1141 tccgcgcagg gcgctgacgt cgttccacat cgtggccccg gcggcgacgg cggcgcgcgc
     1201 gacggcgggc ttgagggtgt cgatcgagat cggcacggcg ctttcggccc ggatcgcggt
     1261 gatcagcggg atcacccgcg ccagctcctc cgcctcatcg acgggcgccg cgcccggccg
     1321 ggtcgactcg ccgccgatgt cgagcatgtc ggcgccctgg gcgaccagct tgcgcgcatg
     1381 gacgagggcg ttctccggcg ccagatgacg cccgccgtcg gagaagctgt cgggcgtgac
     1441 gttgacgatg cccatgacga gagggggtgg cacgacggtg ttgcgtcctt cgagacgccc
     1501 cggatcaggt ccggggctcc tcaggatgac ggaaatcggg ggcggcacaa accgccgtcc
     1561 tggtgacggg tgcgtggttc gacacgcccg gaacaggtcc gggctgctca ccatgacgta
     1621 gcctgaagca cgtcatcctg agcagcgcga agcgcgtgtc gaaggacgca ggaaagccta
     1681 ctccgcgtcc cgcgccacca cgccgccggg gcggggcgac aggggcaccg aggcgctggg
     1741 cccgctgggg cgcttctgct ccggctcgtc ccggttgggc atgacgccct tcatcacatt
     1801 ggcgatttcc tcgccgctga gcgtctcgaa ctcgagcagg gccttggcca gcttgtgcag
     1861 gtcgtcgagc cgctcggtca ggatgcggcg cgcctcgtcg agcccctcct gcaccagccg
     1921 cttgacctcg ccgtcgatca gccgggccgt ctcttccgag acgttctggg tgcgggccac
     1981 cgaatgaccg aggaacacct cttcctggtt gtcgccatag gccacggtgc cgagcttgtc
     2041 ggaatagccc cactgggtga ccatcgcccg ggccaggccc gtggccgcct tgatgtcgct
     2101 gctggcgccg gaggtgatgt tctccttgcc gaagatcagc tcctcggcga cgcggccggc
     2161 catcatgatc gccaggcggc tggtcatctg ggcgtacttc atcgagtagc gatcgccctc
     2221 gggcagctgc atgaccatgc cgagcgcgcg gccgcgcggc acgatggtcg ccttgtgcac
     2281 cgggtcggcg cagggaacgc tcatcgccac cagggcgtgg ccgccctcgt gataggcggt
     2341 cagcttcttc tcttcctcgt tcatggccat cgagcggcgc tcggcgccca tcatgacctt
     2401 gtccttggcc atttcgaagt cgtcgtgggt gaccatgcgg cggttcttgc gcgcggccag
     2461 cagggcggcc tcgttgacca ggttggccag atcggcgcca gagaagccgg gggtgccgcg
     2521 ggccagggtg cgcacgtcga catcggccgc caggggcacg ttgcgcatgt gcacgcgcag
     2581 gatcttctcg cggccggtca cgtcggggtt gggcaccacg acctggcggt cgaagcggcc
     2641 cggccgcaac agggccgggt ccagcacgtc gggacggttg gtcgcggcga tcaggatgat
     2701 gccctcgttg gcctcgaagc cgtccatctc gaccagcagc tggttgaggg tctgctcgcg
     2761 ctcgtcgttc ccgccgccga ggcccgcgcc acggtggcgg ccgacggcgt cgatctcgtc
     2821 gatgaagatg atgcaggggg cgttcttctt ggcctgttcg aacatgtcgc gcacgcggct
     2881 ggcgccgacg ccgacgaaca tctccacgaa gtccgagccg gagatggtga agaagggcac
     2941 gccggcttcg ccggcgatgg cccgcgccag cagcgtcttg ccggtgcccg gagggccgac
     3001 cagcagcgcg cccttgggaa tcttgccgcc caggcgctgg aatttggccg gatccttgag
     3061 gaagtcgacg atctcggtca ggtcgtcctt ggcctcctcg acgccggcga cgtcctcgaa
     3121 ggtcacgcgg ttcttgttct cggtcagcag ccgggccttg gacttgccga agcccatcgc
     3181 gccccgcgcg ccgccctgca tctggcgcat gaagaaaatc cacacggcga tcagcagcag
     3241 gatcgggaag gcctggatca ggatgttgag cagcagcgag cgctcgccgg tcttgaaggc
     3301 gatgttgacc ccgcgggcct ccatccgctt gaccaggtcg ctggagtcgg ccggcacgac
     3361 gaccgtgtag gccttcttgt cctggctcac gacatcgacc gtcgcgccgc ggaagacagc
     3421 cgaccggatc tggcccccgt cgacctggct gagcaggtcg gaatagtgga cttccgcggc
     3481 gggagtcgtt cccggcgcgc cgccccgttg caccaccatg ctccagacgc cggcggcgat
     3541 gatgacgacg accgcccaga tggccaggtt ccgcaggttc attcgatcag acttcctgtt
     3601 cttaccgtcc ccaagatagg gcggccgggc ggttttcgcc acgcgcgcga ttcgtcacgg
     3661 ggcgggttcg ctgtctaccc gccccatggc cgcgtcgaaa cgggccagtg cgagcgcgcg
     3721 gacgcggacc gtcgggcgct ctgcaaggat cgggcaagtc acgccgccgt cccggtcgat
     3781 aaccgccggc aaggccggac gggctgccgc ggggatggcc gacagccgct tccgctcggc
     3841 ttccggcaag cgggcggcgt ggccggccag cgcggtgacg cg
//
LOCUS       DAORNX010000290         3862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17692_length_3862_cov_6.814289, whole genome shotgun sequence.
ACCESSION   DAORNX010000290 DAORNX010000000
VERSION     DAORNX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17692_length_3862_cov_6.814289"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..799
                     /locus_tag="PLO65_12025"
     CDS             383..799
                     /locus_tag="PLO65_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29018.1"
                     /translation="MTRISLALAAGAALSVLAWQAPAAAMGPTFDPKCFADVGWEANP
                     ESESIKVWGCRPAAEVPAANAAGWIEYDRPQVNGSDGGFIMGRLVNVGADGTLIFDVE
                     DNGGGSGTFGYHVIGKPNAEGVLIPSTVHIVALPGD"
     gene            complement(796..2094)
                     /gene="guaD"
                     /locus_tag="PLO65_12030"
     CDS             complement(796..2094)
                     /gene="guaD"
                     /locus_tag="PLO65_12030"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRD29019.1"
                     /translation="MSDVRAWRAALVHCLDDPAQAGAEACVHHPDGLLLVEDGHVAAC
                     GPWAELGPTLAADVPVERLPGRILSPGFVDTHIHFPQIDMVAAFSGQLLDWLETRTFP
                     AERAFADRAHADAAARAFLDELLRNGTTTAMVFGSVHRAGVEALFEEALARNMRLIAG
                     KSLMDGGPEGLRDTVESGREETEGLIRDWGRRGRLGYAVTPRFALSSSPAQLASAGEI
                     VRRHDHVWMQTHLAENAREVETVGRTFPEARDYLDVYDRCGLVTGRSVFGHGIHVTDR
                     SLHRLAQAGAAIAHCPTSNLFLGSGLFDLARARAHGVTVGLGTDVGAGTSFSLLRTAA
                     SACDAAQMRGRPLDPLAAFYLATLGGARALRLDDRIGSFAVGREADFVALDPAATPLL
                     ARRLAGASDIRDILFALMVLGDDRVVSDVWLAGARAHARD"
     gene            complement(2145..3023)
                     /locus_tag="PLO65_12035"
     CDS             complement(2145..3023)
                     /locus_tag="PLO65_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HRD29020.1"
                     /translation="MASALRDRLDAAARDYVQPAGMPAFDFGAPAGEPALASADSVSW
                     RVFANPVALFIGGVTAVLLELAEPRVRSGVWDHTSFRTDPLPRMKRTGLAAMVTVYGA
                     RSKAEAMIAGIGRMHGRVTGVTPAGAPYRADDPELLDWVQATASYGFMEAYHRFVRPL
                     TGAERDRFYAESAPAARLYGATGAPTSVAALETCFETMRPKLERSDIVFEFLAIMHRT
                     AILPGPLRAAQGLFIRAAVELLPAWTRETLGLGRAWGLRPWEAGLVRAAGVMAERMVL
                     PSSPREQARRRLGLAG"
     gene            3127..>3862
                     /locus_tag="PLO65_12040"
     CDS             3127..>3862
                     /locus_tag="PLO65_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29021.1"
                     /translation="MNLDLTPEELAFRDEVRAFLDENLTPDLRQAGRMVTSVFIDKQF
                     SIPWQRILHARGWVAPSWPVEYGGCGWNEMQRYIFAAECTRAGAPGLSPMGLRMVGPC
                     IMKYGTPEQKAFYLPRILAGDDYWCQGYSEPGSGSDLASLQMRAESDGDHYVLNGSKI
                     WTTHAHFANRMFCLVRTWAGGKPQQGITFLLLEMDTPGIEVKPIITLAGEHEVNQVFF
                     DNVRVPKSGRLGEENDGWTVAKYLLEF"
BASE COUNT          632 a         1352 c         1353 g          525 t
ORIGIN      
        1 gcgccgggcg atgagcccgg cagccagacc ttggccacga acagggcgcc cagacccagg
       61 agggcggacg cgccgagact ggcgatggtg gcgactcgca ttctgcggaa ccccgtaaac
      121 gagcggccga tttcccggcg ctctccccga cactgaaatc aggattacag ttcagctctt
      181 gaaatatatt caacgaatac aatgaaggtc cgtaaaccct gctcgtgcgg caagtattag
      241 gcttaaccgt cgtgaaccat ggcgctgccc gcgcccggcc tgttccacat ttcgggtagg
      301 gtgggcgttg tcgccgtggg cgtccggtcg tcccatgcgg tccgccgcgc cgacgggggc
      361 cgcggcggat cgggacaaga ccatgacacg catctcactc gccctggccg ccggggcggc
      421 cctctcggtt ctggcctggc aggcgccggc cgcggcgatg gggccgacct tcgatcccaa
      481 atgcttcgcc gacgtcggct gggaggccaa tccggagtcc gagtcgatca aggtctgggg
      541 ctgccgcccg gcggccgagg tgcctgcggc caacgccgcg ggctggatcg aatacgaccg
      601 cccgcaggtg aacggctcgg acggcggctt catcatgggc cgactggtca acgtcggcgc
      661 cgacggcacc ctgatcttcg atgtcgagga caatggcggc ggcagcggca cgttcggcta
      721 ccatgtgatc ggcaagccga acgcggaggg ggtgctcatc ccctccaccg tccatatcgt
      781 ggccctgccc ggcgactagt cgcgcgcgtg ggcccgggcg ccggccagcc agacgtccga
      841 caccacccgg tcgtcgccga gcaccatcag ggcgaacagg atgtcgcgga tatcgcttgc
      901 ccccgccagg cggcgcgcca gcaacggggt ggcggccggg tcgagggcga cgaaatcagc
      961 ctcgcgaccg acggcgaagc tgccgatgcg gtcgtcgagg cggagcgcgc gagccccgcc
     1021 gagcgtggcc aggtagaagg ccgccagcgg gtcgagcggt cgcccgcgca tctgcgccgc
     1081 gtcgcaggct gacgccgccg tgcgcagaag ggagaagctg gtccccgccc cgacgtcggt
     1141 gcccaggccc accgtcacgc catgggcccg cgcccgggcc aggtcgaaga ggccgctgcc
     1201 gaggaacagg ttggaggtcg ggcagtgggc gatcgccgcg cccgcctgcg ccagccggtg
     1261 cagcgaccga tcggtcacat ggatgccgtg gccgaacacc gaccgcccgg tgaccagccc
     1321 gcagcggtca tagacgtcga ggtagtcgcg cgcctcgggg aaggtccgtc cgacggtctc
     1381 cacctcgcgg gcattctcgg ccaggtgcgt ctgcatccag acgtgatcgt ggcggcgcac
     1441 aatctcgccg gccgaggcca gttgggcggg cgacgacgac agggcgaaac gcggcgtgac
     1501 agcataaccg agccggccgc gacggcccca gtcacggatc aggccctcgg tctcttcgcg
     1561 gcccgattcg acggtgtccc tcaggccctc cgggccgccg tccatcagcg acttgccggc
     1621 gatcagccgc atgttccgcg ccagggcctc ctcgaacagg gcctcgacgc cggcgcggtg
     1681 aaccgagccg aacaccatcg ccgtggtcgt gccgttgcgc agcagttcgt cgaggaaggc
     1741 ccgggccgcc gcatcggcgt gggcccggtc ggcgaacgcc cgctcggccg gaaaggtgcg
     1801 ggtctccagc cagtccagca gctgcccgct gaaggccgcg accatgtcga tctgcggaaa
     1861 atggatgtgg gtgtcgacaa agccgggcga caggatgcgg cccggcaggc gctcgacggg
     1921 cacatccgcc gccagcgtcg ggccgagctc ggcccagggg ccgcaggccg ccacatggcc
     1981 atcctcgacc agcagcaggc cgtcggggtg atggacgcag gcttcggccc ccgcctgcgc
     2041 gggatcgtcg agacaatgga ccagggcggc gcgccaggcg cggacgtcgg acatgggcct
     2101 aggttggagc ctgcgtcgcc ctcgctggca aggaaaagcc gagatcagcc ggccaggccc
     2161 agccgcctcc gggcctgttc gcgcgggctc gacggcagga ccatccgctc ggccatcacg
     2221 cccgccgcgc gcaccaggcc agcctcccat ggacgcaggc cccacgcccg gcccaggcca
     2281 agggtctcgc gcgtccaggc cggcagaagc tccaccgccg cgcggatgaa cagcccctgg
     2341 gccgcccgca gcggtccggg caggatcgcc gtgcgatgca tgatggcgag gaactcgaaa
     2401 acgatgtccg accgctcgag cttcggccgc atggtctcga aacaggtctc cagcgcggcc
     2461 acggaggtcg gggcgccggt cgcgccatag agccgggcgg ctggcgcgga ctcggcgtag
     2521 aaccggtccc gctctgcgcc ggtcaggggg cgcacgaagc ggtggtacgc ttccatgaat
     2581 ccataggagg ccgtggcctg gacccagtcc agcagctccg ggtcgtccgc gcgatagggc
     2641 gcgccggccg gggtcacgcc ggtcacccgg ccgtgcatcc ggccgatccc ggcgatcatc
     2701 gcctcggcct tgctgcgcgc gccatagacc gtgaccatcg ccgccaggcc cgtgcgcttc
     2761 atgcgcggca ggggatcggt gcggaagctg gtgtggtccc acacgcctga tctgacgcgc
     2821 ggctcggcca gctccagcag caccgccgtc acgccgccga tgaacagggc cacgggattg
     2881 gcgaacaccc gccaggacac agagtcggcg gacgcgagcg ccggttcgcc tgcgggcgcg
     2941 ccgaagtcga aggccggcat gcccgccggc tggacatagt cgcgggccgc ggcgtccagg
     3001 cggtcacgaa gggcggaagc catggcgtcg atcctcgtga aaacagtttg cgtcacgtgg
     3061 cgtccaccgg tctaggcgaa ccgacggcag tctgatcagt tgccgcaacg ggatggaaca
     3121 gcacccatga atctcgacct gacgcccgag gaactggcct tccgcgacga ggtccgcgcc
     3181 tttctcgacg aaaacctgac gcccgacctg cgccaggccg gccggatggt caccagcgtc
     3241 ttcatcgaca agcagttctc cattccctgg caaagaattc tccacgccag gggctgggtc
     3301 gcgccaagct ggccggtcga atacggcggc tgcggctgga acgagatgca gcgctacatc
     3361 ttcgcggccg aatgcacgcg ggccggcgcg cccggtctgt cgccgatggg cctgcgcatg
     3421 gtcggcccct gcatcatgaa atacggcacg cccgagcaga aggccttcta tctgccgcgc
     3481 atcctggccg gcgacgacta ctggtgccag ggctattccg agccgggatc gggctcggac
     3541 ctggccagcc tgcagatgcg ggccgagtcc gacggcgacc actatgtgct caacggctcg
     3601 aagatctgga ccacccacgc tcacttcgcc aaccgcatgt tctgtctggt ccgcacctgg
     3661 gccggcggca agccgcagca gggcatcacc ttcctgctgc tggaaatgga caccccgggg
     3721 atcgaggtga agccgatcat caccctggcg ggcgaacacg aggtcaacca ggtgttcttc
     3781 gacaatgtcc gcgtgcccaa gtcgggtcgg ctgggcgagg agaacgacgg ctggacggtg
     3841 gccaagtacc tgctcgagtt cg
//
LOCUS       DAORNX010000291         3856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17729_length_3856_cov_8.347014, whole genome shotgun sequence.
ACCESSION   DAORNX010000291 DAORNX010000000
VERSION     DAORNX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17729_length_3856_cov_8.347014"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1147)
                     /gene="motA"
                     /locus_tag="PLO65_12045"
     CDS             complement(278..1147)
                     /gene="motA"
                     /locus_tag="PLO65_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRD29022.1"
                     /translation="MFQIIGIVLLFGLVFGSYIMSGGKMEVILHAVPHEMMAIGGAGI
                     ASFLISNSLTVVKGAMGGLGKAFAGPKWKASDYRDLLSLLFLLTKTMKSKGVIALESH
                     IEKPADSAIFSRFPRIMKDHFAVDFICDTLRMMTMNLEDPHQVEDAMEKQLEKHHHEH
                     GEPAHALQNLADALPALGIVAAVLGVIKTMGSITEPPAVLGGMIGGALVGTFLGVFLA
                     YGLVGPLASRLKAVVDEEAAFYRIIQSVLVAHLHGNAAQISVEIGRGNVPSGAQPSFL
                     ELEEALSNIPNEA"
     gene            1260..2546
                     /locus_tag="PLO65_12050"
     CDS             1260..2546
                     /locus_tag="PLO65_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29023.1"
                     /translation="MTSPTRTLPKLAPRSFGILFAVSIVVAIGNTGLMSILPAIGRQI
                     GIPDAFMAAIFSLSALLWAVMSPFWARESDKRGRRPLIMLGLTGFAVSMLCCALVVTA
                     GLHHLAPWPVIFVVFLLARALFGLIGSASNPATQAYVAERTSRSERTQMMASLAGAFG
                     LGTVVGPFLAPMFILPVVSLAGPLFSFSLIALCMLIVVWRLLPEESVPRETRRLAGAA
                     LWKDRRLAPFLIYGFLVATCQTAQGYTLGFMIIDKLKVSPVEALGFIAVSMAAGAMAG
                     LLAQWGLIRIFSMGPKTLLRWGVALACVGNLITAFAPDYWTVVAGYAIACLGYGFARP
                     GFTAGASLSVQMHEQAQAAAAIAAVNGLNVIIAPLFVTLYELVHPGPFLLNAVILAGL
                     LVFALRNTILRAAGETADADEEATLAILERSDEGGV"
     assembly_gap    2562..2661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2776..>3856)
                     /locus_tag="PLO65_12055"
     CDS             complement(2776..>3856)
                     /locus_tag="PLO65_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRD29024.1"
                     /translation="GVPAAVLDRELAGLTPDPRASVQDSRQPEFSKPVGDYIKGVVSD
                     ARIAQGRQFRDSLPYLSTIEQRYGAPREIVLAIWAMESAFGKIQGDMDVIRSLATLAA
                     DGRRRGWAEGELIAALKIVGSGEAPRVRLKGSWAGAMGQTQFLPSVFLATAVDGDNDG
                     VRDIWGSTADALYSTGAYMAKAGWKRGESWHREVILPSGFDYSVVEGPKDTPSGWAAK
                     GVRTADGRPWSSADQAAQAGLVLPSGAAGPAFLVFPNHMAIRRYNNSTSYALGVGLLA
                     DRFAGGGPLVTPWPYEVPLSLADRMAAQTALAQLGFNPGPADGIVGSGTRTALRGWQQ
                     ARGLPADGYLSIDLVRKLRAEAGLQ"
BASE COUNT          523 a         1284 c         1314 g          635 t
ORIGIN      
        1 gtcggtcgcc gtggcggcgg cggcgtcggc ggtcgcggcg gcgtcttcgg ccttttcagc
       61 gggcttgccg caggcggcga cggagagggc ggcgacggcg gcgccggcga cgagaagctt
      121 acggagcata tcggaggtca tgtcctggag tcccctgtga ggagttttta aagcgcccca
      181 tcgggggagc gcagtggagg actatgccgc tctctgcgcg gagcgcaagg cctgacttca
      241 cgcccctgtg atcacttttt tcgatcggcg aaaggcctca ggcctcgttc ggtatgttgg
      301 acagggcttc ctcgagttcg aggaagctcg gctgggcccc ggacgggacg ttgccgcggc
      361 cgatctcgac cgagatctgg gcggcgttgc cgtgcaggtg cgccaccagc accgactgga
      421 tgattcggta gaaggcggcc tcctcatcga ccaccgcctt gagccgcgag gccagcgggc
      481 cgaccaggcc gtaggcgagg aacacgccga ggaaggtgcc gacgagggcg ccgccgatca
      541 tgccgccgag cacggcgggc ggctcggtga tcgagcccat ggtcttgatc acccccagca
      601 cggcggcgac gatgcccagg gcgggcaggg cgtcggcgag gttctgcagc gcgtgggccg
      661 gttcgccgtg ttcgtggtga tgcttctcga gctgcttctc catcgcgtcc tcgacctggt
      721 gcggatcctc caggttcatg gtcatcatcc gcagcgtgtc gcagatgaag tcgacggcga
      781 agtggtcctt catgatccgc gggaagcgcg agaagatggc gctgtcggcg ggcttctcga
      841 tgtggctttc cagcgcgatg acgcccttgg acttcatcgt cttggtcagc aggaacagca
      901 gcgacagcag gtcgcgatag tcactggcct tccatttggg cccggcgaac gccttgccca
      961 ggccacccat ggcccccttg accacggtca gcgagttgga aatcaggaac gaggcgatgc
     1021 cggcgccgcc gatggccatc atctcgtgcg gcacggcgtg gaggatgacc tccatcttgc
     1081 cgccggacat gatgtagctg ccgaacacca ggccgaagag cagcacgatg ccgatgatct
     1141 ggaacatgaa ggagagccgc tctgcgaggt taacggcggc aggatcgggg ttaatgctta
     1201 atgggcggtg tgcgcggcgg ccgttgcgcg ccggtttcga cgcgccctag actgcgatca
     1261 tgacctcacc gacgcgaacc ctgccgaagc tcgccccgcg gtcgttcggc atcctgttcg
     1321 cggtctccat cgtggtggcc atcggcaaca ccggcctgat gtcgatcctg ccggcgatcg
     1381 ggcggcagat cggcattccc gacgccttca tggcggcgat cttttccctg tcggccctgt
     1441 tgtgggcggt gatgtcgccg ttctgggccc gcgagtccga caagcgcggt cgccggccgc
     1501 tgatcatgct gggtctgacc ggattcgccg tgtcgatgct ctgctgcgcc ctggtggtca
     1561 cggccgggct gcaccatctg gcgccctggc cggtgatctt cgtcgtcttc ctgctggcgc
     1621 gagccctgtt cggcctgatc ggctcggcct cgaacccggc gacccaggcc tatgtcgccg
     1681 agcgcacctc gcggtccgaa cgcacccaga tgatggccag cctcgccggg gccttcggcc
     1741 tcggcacggt ggtcggcccg ttcctcgcgc cgatgttcat cctgccggtg gtgtcgctgg
     1801 ccgggccgct gttcagcttc tcgctgatcg ccctgtgcat gctgatcgtg gtctggcggc
     1861 tgctgcccga ggagtcggtc ccgcgcgaga cccgccgcct cgccggcgcg gccctgtgga
     1921 aggacaggcg gctcgcgccg ttcctgatct acggctttct cgtcgccacc tgccagacgg
     1981 cccagggcta caccctggga ttcatgatca tcgacaagct caaggtctcg ccggtcgagg
     2041 ccctcggctt catcgccgtg tcgatggccg ccggcgccat ggccggcctg ctggcgcagt
     2101 ggggcctgat ccgcatcttc tccatgggtc ccaagaccct gctgcgctgg ggcgtggccc
     2161 tggcctgcgt gggcaacctg atcaccgcct tcgctcccga ctactggacc gtggtggccg
     2221 gctacgccat cgcctgtctc ggctacggct tcgcccgccc gggattcacc gccggcgcct
     2281 cgctgtcggt gcagatgcac gaacaggccc aggccgcggc ggccatcgcg gcggtcaacg
     2341 gactgaacgt gatcatcgcg ccgctgttcg tgacgctgta cgagctggtc catcccggcc
     2401 cgttcctgct caacgcggtg attctcgccg gcctgctggt gttcgcgctc aggaacacca
     2461 tcctgagggc cgccggcgaa accgcggacg cggacgagga ggcgaccctg gccattcttg
     2521 aacgcagcga cgagggcggg gtctagcgcc cgacgccagt cnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn ncctggccat tcttgaacgc agcgacgagg gcggggtcta
     2701 gcgcccgacg ccagtccgcc cccgatcgcg ctggagggga caggataacc tgtcccctgt
     2761 gggcccctgt cggccctatt gcagccctgc ctcggcccgc agcttcctca ccagatcgat
     2821 cgacaggtag ccgtcggcag gcaggccgcg ggcctgctgc cagccgcgca gggccgtgcg
     2881 ggttcccgag ccgacgatgc cgtccgccgg gcccgggttg aagcccagct gggccagcgc
     2941 cgtctgggcg gccatgcggt cggccagcga cagcgggacc tcgtagggcc agggcgtgac
     3001 cagggggccg ccgccggcga agcggtcggc cagcagaccg acgccgagcg catagctggt
     3061 ggaattgttg taccggcgga tggccatgtg attgggaaac accaggaagg ccggccccgc
     3121 cgcgccggag ggcaggacca gcccggcctg ggcggcctgg tcggcgctgc tccacggccg
     3181 tccgtcggcc gttctgacgc ccttcgcggc ccatccggac ggcgtgtcct tcgggccctc
     3241 gacgaccgaa tagtcgaagc cggacggcag gatgacctcg cgatgccagc tttcgccgcg
     3301 cttccagccg gccttggcca tataggcgcc ggtcgaatac agggcgtcgg cggtcgagcc
     3361 ccagatgtcg cgcacgccgt cgttgtcgcc atcgacggcg gtggcgagga ataccgacgg
     3421 caggaactgg gtctggccca tggctccggc ccagctgccc ttgagccgga cccgcggggc
     3481 ctcgcccgag ccgacgatct tcaacgcggc gatcagttcg ccctcggccc agccgcgccg
     3541 ccggccgtcg gcggccaggg tggccaggga gcggatcacg tccatgtcgc cctggatctt
     3601 gccgaaggcc gattccatgg cccagatcgc cagcacgatc tcgcgcgggg cgccgtagcg
     3661 ctgctcgatc gtcgacaggt agggcaggct gtcgcggaac tgccggccct gggcgatgcg
     3721 ggcgtcgctg accacgccct tgatgtagtc gccgaccggc ttggagaatt cgggctgcct
     3781 cgaatcctgc acggaggccc gcggatcggg ggtcaggcca gccagctcgc ggtcgagcac
     3841 cgccgccggc acgccc
//
LOCUS       DAORNX010000292         3848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17784_length_3848_cov_6.607698, whole genome shotgun sequence.
ACCESSION   DAORNX010000292 DAORNX010000000
VERSION     DAORNX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17784_length_3848_cov_6.607698"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLO65_12060"
     CDS             complement(<1..725)
                     /locus_tag="PLO65_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRD29025.1"
                     /translation="MTDTANAARRIGPWLATVLVAGNMIGSGVFLLPATLAAIGSSTI
                     IGWIIASGGAMLLAGVFALLAILKPTPDGLVDYPAQGLHPFFGFANWLLYWLANWAGV
                     PAVALAITGYLSFFIPALKEPLPGVWATIGFIWLMTLANIIGAKTVTRLGGMTLLIGL
                     APIVAATVLGLMAFDADTFAAGWNVTGKPLAQTVGVSLAPIFWAFLGFESATMAAAVV
                     KDPARNIPRAALGGVGLAAAVYMG"
     gene            833..1747
                     /locus_tag="PLO65_12065"
     CDS             833..1747
                     /locus_tag="PLO65_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29026.1"
                     /translation="MSFDTPLTLVGPLRAPRQMLADQEYGGHSSIHDDAMAEKLGFRA
                     GPIEGPTHFSLFPPLLATIWGNAWFERGCLSAHYQNMVVEGEEVRAIVEIPPEGATRT
                     LVRAEKADGTPVLEASASLGPDHGQTLLEARMAALRPPGRLLILEDLTVGMAGAADEL
                     VKMDPDQHMGALYPFSLNEKLARITETSPWYSDPASSPWGRAIIPLEMVSVLAEYSSH
                     AAKFPVKGPAVGLFADQEIRMIDGPLFVGETYLIRREIAALADSRRTESYWVRSRIFD
                     ESGQRQVAEMLLNHATLKDSYAAYADAV"
     gene            1775..2392
                     /locus_tag="PLO65_12070"
     CDS             1775..2392
                     /locus_tag="PLO65_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD29027.1"
                     /translation="MKLYNSVGPNPRMVRMFAAEKGLTLPTVEIDLLGGENRRPAYAG
                     GVNVAGQTPALQLEDGQVISEITVICEYLEEKHPTPALIGATAEARAETRMWTRRIDL
                     NICEPLANGFRASEGRGLFESRMKLVGAEGAAELKAIARDRILWLDGLMAGRPFICGD
                     RFSLADILLFAFLDFGATVGQPVPAEAAWVKAWFERVKARPSAAA"
     gene            2478..2864
                     /locus_tag="PLO65_12075"
     CDS             2478..2864
                     /locus_tag="PLO65_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29028.1"
                     /translation="MSRWIAALLALLLAANSVFQLIAPLAWYDAVPGVALTGAFNPHF
                     VRDIGMAYLVVVLGLAWFAWRPHQGWPALVCAAAFLALHATIHVGDGACGSAPLNDFT
                     RDLPGVFLPALLTGWIAWRSRPTEGA"
     gene            2867..3421
                     /locus_tag="PLO65_12080"
     CDS             2867..3421
                     /locus_tag="PLO65_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29029.1"
                     /translation="MLKAFFRSQIRKFETQWGYDAAYMRDLLDAGTGTFLRFSFVPAL
                     GHSKDAPAAAMAAAGIVGTLAEDCGPCTQIGVDIAAANGVKPAILRAILAGDRAAMGE
                     TAALGYDFARAVLDRHLEDSDEVRDEIVRRWGKKAVVALSLALTTARMYPTLKYGLGH
                     GKACSRVIVAGEPAPFHRPEALAA"
     assembly_gap    3473..3640
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3641..>3848
                     /locus_tag="PLO65_12085"
     CDS             <3641..>3848
                     /locus_tag="PLO65_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRD29030.1"
                     /translation="PLIEEVADDPVERAEDVSVAFLLALERLSPLERAVFLLHDVFDQ
                     DYAAVGAALGKSEPAVRQLASRART"
BASE COUNT          566 a         1313 c         1265 g          536 t
ORIGIN      
        1 cccatgtaga ccgccgccgc caggccgacg ccgccgagcg ccgcccgggg gatgttgcgc
       61 gcggggtcct tgaccacggc cgcggccatg gtcgcgcttt caaacccgag gaaggcccag
      121 aagatcgggg ccagcgagac gcccacggtc tgggccagag gcttgccggt gacgttccag
      181 ccggccgcga aggtgtccgc gtcgaaggcc atcaggccca ggaccgtcgc ggcgacgatc
      241 ggcgccaggc cgatcagcaa ggtcatcccg cccagccgcg tgacggtctt cgcgccgatg
      301 atgttggcca gggtcatcag ccagatgaac ccgatcgtgg cccagacgcc ggggaggggt
      361 tccttcaggg ctgggatgaa gaacgacaga tagccggtga tggccagggc gaccgccggc
      421 acgccggccc agttggccag ccagtagagc agccagttgg caaagccgaa gaagggatgc
      481 aggccttgcg ccgggtagtc gaccaacccg tccggggtcg gcttgaggat agccagcagg
      541 gcgaacaccc ccgccagcag catcgccccg cccgaggcga tgatccagcc gatgatggtc
      601 gatgagccga tcgccgccag cgtcgccggc agcaggaaga ctccggagcc gatcatgttg
      661 ccggccacca gcacggtggc cagccagggt ccgatgcgcc gggcggcgtt ggcggtatct
      721 gtcatcgggg aagtggtccg cagttcgacc ggtccgtcaa cgaccgttgt tcgcttgacg
      781 ccccgcgcct ggcgccgctc aaaggcgggg catccctgag cgagagaccg ccatgagctt
      841 tgacacgccc ctgaccctgg tgggtcccct gcgcgcaccg cgccagatgc tggcggacca
      901 ggaatacggc ggccacagct cgatccacga cgacgccatg gccgagaagc tcggcttccg
      961 cgccggaccg atcgaggggc cgacccactt cagcctgttc ccgccgctgc tggcgacgat
     1021 ctggggcaac gcctggttcg agcgcggctg cctgtcggcc cactaccaga acatggtcgt
     1081 cgagggcgaa gaggtgcggg ccatcgtcga gattccgccg gaaggggcga cgcgcaccct
     1141 ggtccgggcc gagaaggccg acggcacgcc agtgctggag gcctcggcca gccttggtcc
     1201 cgaccacggc cagaccctgc tggaggcccg catggccgcc ctgcgcccgc ccggcaggct
     1261 gttgatcctc gaggacctga cggtcggcat ggccggcgcc gccgacgagt tggtgaagat
     1321 ggaccccgac cagcacatgg gcgcgctcta tccgttttcg ctcaacgaga aactggcccg
     1381 gatcaccgag acttcgccct ggtacagcga ccccgcgtcc tcgccctggg gccgggcgat
     1441 catcccgctg gagatggtca gcgtgctggc cgaatactcc agccatgccg cgaagttccc
     1501 ggtgaagggg cccgccgtcg gcctgttcgc cgaccaggag atccgcatga tcgacgggcc
     1561 gctgttcgtc ggcgagactt atctgatccg ccgcgagatc gccgccctgg ccgacagccg
     1621 ccgcaccgag tcctactggg tccgcagccg catcttcgac gagtcgggcc agcggcaggt
     1681 cgccgagatg ctgctcaacc acgccacgct caaggacagc tacgccgcat atgcggacgc
     1741 ggtctaggtc gacgccagaa cagggaaacg caccatgaaa ctctacaact ccgtcggtcc
     1801 gaacccgcgc atggtgcgca tgttcgccgc cgaaaagggc ctcaccctgc cgacggtcga
     1861 gatcgacctg ctcggcgggg agaaccgccg gccggcctat gccggcgggg tcaatgtcgc
     1921 cggccagacc cccgccctgc agctcgagga cggacaggtg atcagcgaga tcaccgtcat
     1981 ctgcgagtac ctcgaggaaa aacacccgac cccggcgctg atcggcgcca cggccgaggc
     2041 gcgggccgag acccgcatgt ggacccgccg catcgacctc aacatctgcg agccgctggc
     2101 caacggcttc cgggcgtcgg aaggccgcgg tctgttcgaa agccgcatga agctggtcgg
     2161 ggccgagggc gccgccgagc tcaaggccat cgcccgcgac cgcatcctgt ggctggacgg
     2221 cctgatggcc ggtcgcccgt tcatctgcgg cgaccgcttc agcctggccg acatcctgct
     2281 gttcgccttc ctcgatttcg gggccaccgt cggccagccg gtcccggccg aggccgcctg
     2341 ggtcaaggcc tggttcgagc gggtcaaggc caggcccagc gcggcggcct gaccagccgc
     2401 ccggggggat tttcgagagt cgcatgtcac gacatgcgcg gctgcctcgt ctggtcttca
     2461 gacaacggag cccgcccatg tcccgatgga tcgccgccct gctggccctg ctgctggcgg
     2521 ccaacagcgt cttccagctg atcgcgccgc tggcctggta cgacgccgtg ccgggcgtgg
     2581 ccctgaccgg ggcgttcaac ccgcacttcg tccgcgacat cggcatggcc tatctggtcg
     2641 tggtgctggg ccttgcctgg ttcgcctggc ggccgcacca gggttggccg gcgcttgtct
     2701 gcgcggcggc ctttctggcc ctgcatgcca cgatccacgt cggcgacggc gcttgcggct
     2761 ccgccccgct caacgacttc acccgcgacc ttcccggcgt cttcctgccc gccctgttga
     2821 cgggctggat cgcctggcgg tcgcgcccga cagagggagc ctgaccatgc tcaaagcctt
     2881 cttccgcagc cagatccgca agttcgagac gcaatggggt tatgacgcgg cctacatgcg
     2941 cgacctgctc gacgccggga ccgggacctt cctgcgcttc agcttcgtgc cggcccttgg
     3001 tcattcgaag gacgccccgg cggcggccat ggccgcggcg gggatcgtcg ggaccctggc
     3061 cgaggactgc gggccctgca cccagatcgg cgtggacatc gccgcggcca acggcgtgaa
     3121 gccggccatc ctgcgggcca tcctggccgg cgaccgggcg gccatgggcg agaccgccgc
     3181 cctcggctac gacttcgccc gggccgtgct cgaccgccac ctggaggatt ccgacgaagt
     3241 ccgcgacgag atcgtccgcc gctggggaaa aaaggccgtg gtggccctca gtctcgccct
     3301 gacgaccgcg cggatgtatc ccacgctgaa gtacggcctc ggccacggga aggcctgttc
     3361 ccgcgtgatc gtggccggcg agccggcgcc cttccaccgg ccggaggccc tggcggcatg
     3421 accgacgcca cgaccgtgtt cgagcaggaa cggccccgcc tgaagcggct ggnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccgctgat cgaggaggtc
     3661 gccgacgatc cggtcgagcg cgccgaggat gtgtcggtgg ccttcctgct ggcgctcgag
     3721 cggctttcgc cgctcgagcg ggcggtgttc ctgctgcacg atgtgttcga ccaggactac
     3781 gccgccgtcg gtgcggcgct gggcaagagc gagccggccg tgcgccagct ggccagccgc
     3841 gcccggac
//
LOCUS       DAORNX010000293         3842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17825_length_3842_cov_6.895960, whole genome shotgun sequence.
ACCESSION   DAORNX010000293 DAORNX010000000
VERSION     DAORNX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17825_length_3842_cov_6.895960"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLO65_12090"
     CDS             <1..1041
                     /locus_tag="PLO65_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRD29031.1"
                     /translation="HYLIYAGLGGACALLGIGLMRAVAVVEHGTRRAPIPDWSRPVIG
                     GLLLIPLAMISPQGLSAGHGALHLDLALGASITVIALALVIKSLASILSLGFGFRGGL
                     FFASLFLGTLVGHLFAAGLNQLAGYPLVDVHNAALVGMAALAVAVVGGPMTMAMLVLQ
                     ATHDFPLTGAVLAASLVSSTIVRELFGYSFSTWRLHLRGETIKSARDVGWVKTLTAGR
                     MMRRETRLTPATLTVAEFRRTFPLGSTGRVVLIDGDDRYAGIVVVPAVYAEGVDAAAP
                     VGALAVARDATLTPEMDIGAVMKTFDAAGADELAVLAGDRRVLGLVSETYVRRRYAEE
                     LEKTQRELFGER"
     gene            1111..1818
                     /locus_tag="PLO65_12095"
     CDS             1111..1818
                     /locus_tag="PLO65_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRD29032.1"
                     /translation="MTDLSAFPVTARWPARHPDRLQLYSLNTPNGVKVSIMLEEIGLP
                     YEPHLVDIGANESWTPEFLSLNPNGKIPAIIDPDGPGGRPMGLFESGAILLYLAEKTG
                     HLLPRDPAARYETLQWVFFQVGAIGPMFGQLGYFHKFAGREIADKRPLERYRDESRRL
                     LGVLETRLTGRPWMMGEAFTIADIALIGMVRNLIGYYDAGDLVGFDALSAVPAWLERG
                     LARPAVQRGLQIPARNA"
     gene            1818..2165
                     /locus_tag="PLO65_12100"
     CDS             1818..2165
                     /locus_tag="PLO65_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HRD29033.1"
                     /translation="MTITLHGIKACDTMKKARAWLDGHGVAYAFHDYKAVGIDAATLA
                     RWAGQVGWETLLNRAGTTFRKLPESDKAGLDQARAIALMVAQPSMIKRPVLDVDGRLT
                     VGFKPETYARVFG"
     gene            2199..2624
                     /locus_tag="PLO65_12105"
     CDS             2199..2624
                     /locus_tag="PLO65_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29034.1"
                     /translation="MTDTGVKSGAPRWLRSLAAVLAGFFVVVVLSTAADAAMHATGVF
                     PPADQRMEDPLLFLLALAYRGLFTLLGGCVTARLAPASPMRHALALAALGLLAGMLGI
                     MAALASDLGPLWYAIAVAVSGPPCVVLGGWMVARRATPA"
     gene            2756..2953
                     /locus_tag="PLO65_12110"
     CDS             2756..2953
                     /locus_tag="PLO65_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29035.1"
                     /translation="MEDLFRSYWWLLFPFAWMIISGFRGWLAHQRRRDSLDLIRHLSD
                     SGREIPAGLIEKLGGRAGSGV"
     gene            2957..3373
                     /locus_tag="PLO65_12115"
     CDS             2957..3373
                     /locus_tag="PLO65_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29036.1"
                     /translation="MEDLFRSYWWLLFPLAWFIVGGWQSWLNYRKHRDNLDVIRRYAD
                     SGKDIPAGLLDKLNSPAPDDWDSYDGRRGRRYYRRGYGGWYQVVLFGSLAAGFGYASW
                     TDIYGAGEAFTIVAFVMAAMCLASLVATLTTRTPKD"
     gene            3378..>3842
                     /locus_tag="PLO65_12120"
     CDS             3378..>3842
                     /locus_tag="PLO65_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRD29037.1"
                     /translation="MDAPLVRAARAGSQAAFGRLVVKHQQGLRAFLRRIVGPDADDVA
                     QEALVIAWASLGRLRDDEGFRPWLYGIAWRKGRTLLRSSARAARRDHQWTRDQDGEGG
                     GLDPADRMALEAAMAQLPPDQRAAVALCLAQGWTHAEAAGALAIRQDIRLLPH"
BASE COUNT          515 a         1346 c         1356 g          625 t
ORIGIN      
        1 cactatctca tctacgccgg cctgggcggc gcctgcgcgc tgctcggcat cggcctgatg
       61 cgggccgtgg ccgtggtcga gcacggcacc cggcgggctc cgatcccgga ctggagccgg
      121 ccggtcatcg gcgggctgct gctgatcccg ctggcgatga tctcgccgca gggcctttcc
      181 gccggtcacg gcgcgctgca tctcgacctg gcgctgggcg cgtcgatcac cgtcatcgcc
      241 ctggccctgg tgatcaagag cctggcctcg atcctgtcgc tcggcttcgg cttccgcggc
      301 ggcctgttct tcgcctcgct gttcctcggc accctggtcg gccacctgtt cgccgcggga
      361 ctgaaccagc tggccggcta tccgctggtc gatgtccaca acgcggccct ggtcgggatg
      421 gccgccctgg ccgtggcggt ggtgggcggc ccgatgacca tggcgatgct ggtcctgcag
      481 gcgacccacg acttccccct gaccggggcg gtgctggcgg cgtcgctggt gtcctcgacc
      541 atcgtgcgcg agctgttcgg ctattcgttc tcgacctggc ggctgcatct gcgcggcgag
      601 acgatcaaga gcgcccgcga cgtcggctgg gtcaagacgc tcaccgccgg ccggatgatg
      661 cggcgggaaa cccggctgac gcccgcgacc ctgacggtcg ccgagttccg ccggaccttt
      721 ccgctaggct cgaccggccg cgtggtgctc atcgatggcg acgatcgcta cgccggtatc
      781 gtcgtggtgc cggcggtcta cgccgaaggg gtcgacgccg ccgcgccggt cggagccctg
      841 gcggtcgccc gtgacgcgac cctgacgccg gaaatggaca tcggcgcggt gatgaagacg
      901 ttcgacgcgg ccggggccga cgaactggcc gtgctggccg gggaccgtcg ggtgctgggc
      961 ctggtgtccg agacctatgt gcgccgccgc tatgcggagg aactggagaa gacgcaacgc
     1021 gaactgttcg gcgaacgctg acgccggcct tgaaaggtcg gcgcgggggc gccaactcga
     1081 cacggccgga ccgcggcgca ggagtccctg atgaccgacc tctcagcctt ccccgtcacc
     1141 gcccgatggc ccgccaggca tccggaccgg ctgcagctct attcgctgaa cacgcccaac
     1201 ggggtgaagg tctcgatcat gctggaggag atcggcctgc cgtacgagcc gcacctcgtc
     1261 gacatcggcg cgaacgagag ctggaccccc gaattcctgt cgctcaatcc caacggcaag
     1321 attccggcga tcatcgaccc cgacggaccc ggcgggcgac cgatgggcct gttcgagtcc
     1381 ggcgcgatcc tgctctatct cgccgaaaag acaggccatc tgctgccgcg cgatccggcc
     1441 gcccgctacg agaccctcca gtgggtgttc ttccaggttg gggcgatcgg gccgatgttc
     1501 ggccagctcg gctacttcca caagttcgcc ggccgcgaga tcgccgacaa acggccgctg
     1561 gaacgctacc gcgacgaatc ccggcgcctg ctgggggtgc tggagacgcg cctgacggga
     1621 cggccgtgga tgatggggga ggccttcacc atcgccgaca tcgccctcat cggcatggtg
     1681 cgcaacctga tcggctacta tgacgcgggc gaccttgtcg gcttcgacgc gctcagcgcg
     1741 gtgccggcct ggctcgagcg cggcctggcc cggccggccg ttcagcgcgg actgcagatc
     1801 ccggccagga acgcctgatg acgatcaccc tccatggcat caaggcctgc gacacgatga
     1861 agaaggcccg cgcctggctc gacggccacg gcgtggccta cgccttccac gactacaagg
     1921 cggtcggcat cgacgcggcc accctcgcgc gctgggcggg gcaggtcggc tgggagacgc
     1981 tgctcaaccg cgccggcacg accttccgca agctgccgga gagcgacaag gccgggcttg
     2041 accaggccag ggccatcgcc ctgatggtcg cccagccctc gatgatcaag cgaccggtgc
     2101 tcgacgtcga cggcaggctg acggtcgggt tcaagcccga gacgtacgcg cgcgtgtttg
     2161 gctaggccgc ggcagggcgg cgacaggggg aaccgagaat gaccgacacg ggcgtgaaat
     2221 ccggcgcgcc gcgctggctc aggagcctgg cggccgtcct ggcgggcttc ttcgtggttg
     2281 tcgtgctgtc gacggcggcc gacgcggcca tgcacgccac gggcgtcttc ccgccggccg
     2341 accagcggat ggaggacccg ctgctgttcc tgctggcgct ggcctatcgc ggtctgttca
     2401 cccttctggg cggctgtgtg acggcccggc tcgcgccggc ctcgccgatg aggcatgcgc
     2461 tggcccttgc ggcccttggc ctgctggccg ggatgctggg aatcatggcc gcgctggcca
     2521 gcgacctggg ccccctgtgg tacgcgatcg ccgtggccgt ctccggtccg ccctgcgtgg
     2581 tgctcggcgg ctggatggtg gcgcggcgcg ccacgccggc ctgaccggag tcccgcgggc
     2641 cggcggtgaa tccgcagggt cgagactttt ttcacgccgc ctgcatccgg accggggtcc
     2701 ggcggcgact acttcacaaa ggacgcaggg acgcgtcccg gctggaggca aattgatgga
     2761 agacctgttc cgttcctact ggtggctgtt gtttccgttc gcctggatga tcatcagcgg
     2821 cttccgcggc tggctggccc atcagcgccg ccgtgacagt ctcgatctca tcaggcatct
     2881 ctcggacagc ggccgggaga ttccggccgg cctgatcgag aagctcggcg gccgcgccgg
     2941 ttcgggagtc tagaccatgg aagatctgtt ccgttcctat tggtggctgc tgttcccgct
     3001 ggcctggttc atcgtcggcg gctggcagag ctggctgaac taccgcaagc atcgcgacaa
     3061 cctcgacgtc atccgccggt acgccgacag cggcaaggac atccccgccg gcttgctgga
     3121 caagctcaac agcccggcgc ccgacgactg ggacagctac gacggccgtc gtggccggcg
     3181 ctactaccgc cgcggctacg gcggctggta ccaggtggtg ctgttcggct ccctggccgc
     3241 cggtttcggc tacgcctcct ggaccgacat ctacggcgcg ggcgaggcct tcacgatcgt
     3301 cgccttcgtg atggccgcca tgtgcctggc ctcgctggtc gcgaccctga ccacgcggac
     3361 gccaaaggac tgaccgggtg gatgcgccgc ttgtccgggc ggccagggcc gggtcccagg
     3421 ccgccttcgg acgacttgtg gtcaagcatc agcagggcct gcgcgccttc ctgcggcgta
     3481 tcgtcggccc cgacgccgac gatgtcgccc aggaggcctt ggtgatcgcc tgggcgtctc
     3541 tgggccggct gcgcgacgat gaggggttcc gcccctggct ctacggcatc gcctggcgga
     3601 agggccggac cctgcttcgc tccagcgccc gcgccgcccg tcgtgatcac cagtggaccc
     3661 gggaccagga cggcgagggc ggtggtctcg acccggcgga ccggatggcg ctggaggcgg
     3721 ccatggcgca gttgccgccg gaccagcgcg cggcggtggc cctgtgtctg gcccagggct
     3781 ggacccacgc cgaggccgcc ggggcgctgg ccatccgtca ggacatccgc ctgctgccgc
     3841 ac
//
LOCUS       DAORNX010000294         3839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_17855_length_3839_cov_6.511628, whole genome shotgun sequence.
ACCESSION   DAORNX010000294 DAORNX010000000
VERSION     DAORNX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17855_length_3839_cov_6.511628"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLO65_12125"
     CDS             <1..291
                     /locus_tag="PLO65_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29038.1"
                     /translation="AGDGPTGLRLLSDEPVDLLIVDYAMPVMTGAEVARSALALQPTL
                     PILFVSGYAETEALESAVARPIQLLRKPFDSQTLGQAVRAALDASPSPPAAA"
     gene            309..839
                     /locus_tag="PLO65_12130"
     CDS             309..839
                     /locus_tag="PLO65_12130"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD29039.1"
                     /translation="MSRFTGPVRQNGYVVRDVQAAMRHWIEVLGVGPFFYIEKVPVDW
                     FRHRGVTYDIDLSIALANSGDLQIELIQQRNEVPSMYREFAARHGEGLQHMSWWTTTY
                     QADYEAALARGLTVGHEGQINGPNGRFVYFDSDLDHPGTVIELSDVSGTKMRFFDHIR
                     RAAVGWDGSNPVRVVT"
     gene            complement(1398..2189)
                     /locus_tag="PLO65_12135"
     CDS             complement(1398..2189)
                     /locus_tag="PLO65_12135"
                     /inference="COORDINATES: protein motif:HMM:NF023204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRD29040.1"
                     /translation="MKILLTSALALSLLGSGSAFAQTSYPAPDRDRASPAYDYRPGGP
                     RWSRGDRLPPQFRQERYRVTEWRRLGLRQPPRGYWWYRYGDNNYFLAAITTGLILETF
                     YRDDRDQRWNQNYRRTYTYQDDIYYRECRTSSDPAGVLLGALIGGLIGNAAGDGRGSS
                     TAAGVIIGGALGAAMTRDMDCEDRSYAYRSYYNGFNSGRPGARYSWTNPRTRHRGDVV
                     IGNYYYDRSGFRCTTFTQTIYIQGRPQVSRGHACRQPDGTWAVVD"
     gene            complement(2326..3564)
                     /locus_tag="PLO65_12140"
     CDS             complement(2326..3564)
                     /locus_tag="PLO65_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD29041.1"
                     /translation="MDQTLPLQGAPWETLRERMVEMAAGDVKWRDGKAAVYVFNAGAE
                     VERVQKEAYALFASENGLGPAAFPSLARMEKEVLAAGLGLLHGPDGATGAMTSGGTDS
                     ILMAVKAARDHARKVRGRSGPLNIVLPRSAHPAFDKACLVMEIEVRRTPLKDHLADPE
                     AMEAVVDEDTIMIVGSAPCFPFGLIDPIEALGDLAVRRDLWLHVDACVGGYMAPFVRM
                     NGGDVRPFDFEVPGVRSMSADLHKYGYCAKGASTVFFRSEDLKQHMIFEVGDWPAGRM
                     TTPTLAGTRPGGAIAAAWAVMHSLGVEGYRARHGEVTQAREIIEAGVRVRGFSVIGAP
                     QLGILAFTREDVDCMAILGGLYQRGWVTSAITQPRGLHLMLSPVHLTVADRYLADLDE
                     AVAAARGGTAGQRSDVGYAR"
BASE COUNT          574 a         1324 c         1352 g          589 t
ORIGIN      
        1 gcgggcgacg gcccgaccgg cctgcgactg ctgtcggacg aacctgtcga cctgctgatc
       61 gtggactacg ccatgcccgt gatgacgggg gcggaggtcg cccgctcggc cctggccctg
      121 cagccgacgt tgccgatcct cttcgtcagc ggctacgccg agacagaagc cctcgaaagc
      181 gcggtcgccc gccccatcca gctgttgcgc aaaccgttcg actcccagac gctcggccag
      241 gcggtgcggg cggctcttga cgcctcgccg tcccctccgg ccgccgcctg atcagcgagg
      301 actgcgcgat gagtcgattt accgggcccg ttcgccagaa cggctatgtc gtgcgcgacg
      361 tccaggcggc gatgaggcac tggatcgagg tgctgggcgt gggtccgttc ttctacatcg
      421 agaaggtccc ggtcgactgg ttccgtcacc gcggagtcac ctacgacatc gacctgtcga
      481 tcgccctggc caacagtggc gatctgcaga tcgaactgat ccagcagcgt aacgaggtcc
      541 cgtcgatgta tcgcgagttc gcggcccggc acggcgaggg cctgcagcac atgtcctggt
      601 ggacgacgac ctaccaggcc gactacgagg ctgccctggc acgcgggctg accgtcggcc
      661 acgaggggca gatcaacggg ccgaacggcc ggttcgtcta tttcgacagc gacctggatc
      721 acccgggaac ggtgatcgaa ctgtccgacg tcagcgggac caagatgcgg ttcttcgacc
      781 acatccgccg cgccgccgtt ggctgggacg gaagcaatcc ggtccgggtg gtgacctagc
      841 cggctgcgcc gggttcgggg agggcttacg cgcccaaccc actggatttt cacgtcttcc
      901 tcgcgaatgc ccggccgcgt gcggtctgct ttcgctgctg cgccgacgct gcccctatgc
      961 ttcagccatc cccgccgcac ggggagggcg cgaaggcctg cgaccttgcg gcggcggggg
     1021 tgatggttga gcgggattga ccaccggacc ggcagcagtg cgccggccgg ccgccgggcc
     1081 cgcgtcggct aagccggaca atggctgcct accgacgccc ccgtcagaac ggccatcgcg
     1141 gagcgttacg gactgccgca agcctgccca ggactgccct cttgcacccg gcgacagccg
     1201 gggcgctccg cgccacctcc ccaccctcgt ccgggaaacc ggccggggcg gcgaagccgt
     1261 ggccaagcgg cgcccaacgc gggcggtcga ggccgtcatc tctcatcgtg cggacgtggc
     1321 cgagggctcc gattgtccct gtcctcgcgg agacaatcgg agctaaagcg gccccggggc
     1381 cgtgaacatc gatcgtacta gtcgacgacg gcccaggtcc cgtcgggctg ccggcaggca
     1441 tggccgcgcg agacctgcgg ccggccctgg atgtagatgg tctgggtgaa ggtcgtgcag
     1501 cggaagccgg accggtcata gtagtagttg ccgatcacca cgtcgccgcg gtggcgggtg
     1561 cgcgggttgg tccagctgta gcgcgcgccc ggccggcccg agttgaagcc gttgtagtag
     1621 gaccggtagg cgtagctgcg gtcctcgcag tccatgtccc tggtcatggc ggcgcccagg
     1681 gcgcccccga tgatcacccc ggcggcggtg gaactgccac ggccatcgcc ggcggcgttg
     1741 ccgatcaggc cgccgatcaa cgcgccgagc agcacgccgg ccggatcgga cgaggtgcgg
     1801 cattcccggt agtagatgtc gtcctgatag gtgtaggtgc gccggtaatt ctggttccag
     1861 cgctggtcgc ggtcgtcgcg gtagaaggtt tccaggatca ggccggtggt gatcgcggcg
     1921 aggaagtagt tgttgtcgcc gtagcggtac caccagtagc cgcgcggcgg ctggcgcagg
     1981 cccaggcgac gccactccgt cacgcggtag cgttcctgac ggaactgcgg cggcaggcgg
     2041 tcgccacgcg accagcgggg gccgccggga cgatagtcgt aggcagggct ggcgcggtca
     2101 cgatccggcg cgggatagga ggtctgggcg aaggccgaac cgctgccgag cagcgacagg
     2161 gcgagggctg aggtgagaag gatcttcacg tgagttctcc ttttcggcgg acgaccgtca
     2221 cgcgccgtcg gccggcttgc gtttctgggc aaggccactg gagaccagat gcccgatccg
     2281 cgaccgggcg aagttgaggc acatcaagtc aggcgagaat ccactctacc gggcgtagcc
     2341 gacgtcggag cgctggccgg cggtcccgcc ccgagcggcg gcgacagcct cgtcgaggtc
     2401 cgccagatag cggtcggcga cggtcaggtg cacgggcgac agcatcaggt gcagaccgcg
     2461 tggctgggtg atggcgctgg tcacccagcc gcgttgatac agtccgccga gaatcgccat
     2521 gcagtcgacg tcctcgcggg tgaaggccag gattcccagc tgcggcgcgc cgatgacgct
     2581 gaagccccgc acgcgcacgc cggcctcgat gatttcgcgg gcctgcgtca cctcgccgtg
     2641 cctggcccgg tagccctcga ccccaaggct gtgcatcacc gcccaggccg cggcgatggc
     2701 cccgccaggc cgcgtgccgg ccagggtcgg ggtggtcatc cggccggcgg gccagtcgcc
     2761 gacctcgaag atcatgtgct gcttcaggtc ttcggaccgg aagaacacgg tcgaggcgcc
     2821 cttggcgcag tagccatact tgtgcaagtc ggccgacatg gaccgcacgc ccggaacctc
     2881 gaagtcgaag ggccgcacat cgccgccgtt catgcgcacg aacggcgcca tgtagccgcc
     2941 gacgcaggcg tcgacgtgca gccacaggtc gcgccgcacg gcgagatcgc cgagggcctc
     3001 gatcggatcg atcaggccga agggaaagca cggcgcagac cccacgatca tgatggtgtc
     3061 ctcgtcgacg accgcctcca tggcctccgg atcggcgagg tggtccttca gcggcgtgcg
     3121 acggacctcg atctccatca caaggcaggc cttgtcgaag gccggatgcg ccgagcgggg
     3181 cagcacgatg ttgagcggtc cgctccggcc gcggaccttg cgggcgtggt cgcgggccgc
     3241 cttgaccgcc atcaggatgg agtcggtgcc gccgctggtc atggcgccgg tcgcgccgtc
     3301 gggaccgtgc agcaggccca ggccggccgc cagcacctcc ttttccatcc gcgccaggct
     3361 tggaaaggcc gccgggccca ggccgttctc cgaggcgaac agggcgtagg cctctttctg
     3421 cacccgttcg acctccgcgc cggcgttgaa gacatagacg gcggccttgc cgtcgcgcca
     3481 tttgacgtcg cccgcggcca tctcgaccat gcgctcgcgc agtgtctccc agggcgcgcc
     3541 ctgcagcgga agtgtctggt ccatgggagt ctccggcccg atccggtccc agcctagccg
     3601 tccgcgccgc cgcgtcgagc ccgcaacgcc gttgtcgcgg ccttaaccac gctgaaaacc
     3661 ctttgcgcca tgtttgagcc ggtcgaaggg gaggaaacat gtcagcgtcg cgccgaaagc
     3721 tgtcgccggc gggcctcgcg gtcctgctgg cctgtctggc cctggccgcc tgcgcctcgc
     3781 cgcgctacgc cgttcgcggc ccgtcgccct cggggcctgg gcccaccgtc gcgcccggc
//
LOCUS       DAORNX010000295         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18060_length_3807_cov_8.974147, whole genome shotgun sequence.
ACCESSION   DAORNX010000295 DAORNX010000000
VERSION     DAORNX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_3807_cov_8.974147"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="PLO65_12145"
     CDS             <1..2008
                     /locus_tag="PLO65_12145"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRD29042.1"
                     /translation="MEGKAVLFKRFADVDAIDVELSTEDPDEIITVVKTIGATFGGIN
                     LEDIKSPECFIIEQRCQELLDIPVFHDDQHGTAIICAAGLINACYITGKKIEDVKVVL
                     NGPGAAGIASLELIKAMGVRPENCIACDSKGVLYQGRTDGMNQWKSAHAVDTDKRTLA
                     EAVAGADVLLGLSAKGAFTREMIASMAPNPVIFAMANPDPEITPEEVHAVRSDAIMGT
                     GRSDYPNQVNNVLGFPYIFRGALDVRARRVNHEMKIACANALAMLAREDVPDEVAAAY
                     HGRQLKFGPQYIIPSAFDPRLIWYIPPFVAQAAMDTGVARRPIADMDAYRASLAQRLD
                     PTAAFLQKISGAVMNGPKKRIVFAEGEEPSVIRAAYAFQAQGYGEAILCGREELVKEN
                     MKLAGLDPDEVQLQIVNARLSDRNPDYVEHLYQRLQREGYLKRDVQRLINQDRNSFAA
                     SMVALGDADAMVTGVTRSYDQVLEEVLRVMDPAPGGRVMGMSIVLAKGHTVFIADTNV
                     TEMPEADELVEIACEAARAVRTLGFTPRVAFMSYSTFGNPMGLRSEKVREAVAMLDEM
                     DGVDFEYEGEMPPELALEPESRDNFPFMRLTGPANVLIMPAIHSASISTKLVQALGGA
                     TVLGPILLGLNKSVQIAPLSASVSKILNMALMAAYDQPLEPAAE"
     gene            2118..3431
                     /locus_tag="PLO65_12150"
     CDS             2118..3431
                     /locus_tag="PLO65_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRD29043.1"
                     /translation="MTRVSDAAIGVDFGTTNTVLAFDQGGGQAGLAGFSHDGQPFNAF
                     RSALSFHAPADDPAGRVVEAGPWAIDAYVEEPLETRFIQSFKSYAASPLFTDTEILRR
                     RFSFEDLLSAFLLKVREHGGADLADLPKPVIVGRPVAFAGLRPDPALALTRYETAFRR
                     LGFTDIRYVYEPVAAAFFFARRLEGDATVLVGDFGGGTSDFSLVRFTHKGGRVRSQAL
                     SNAGVGVAGDAFDYRIIDHLVSPKLGKGSLYKAFDNVIPLPNRYFTAFARWEQLALLR
                     ASRDMREIRRLVRDAVEPEKLERLVEVLDDNHGYRLYQSVSRLKEALSGAEEAVFSFK
                     GGSVEIEGPVKRASFDRWIQPELTAIEGAVDQALARAGLTEAGVDRVFLTGGSSFVPA
                     VRAIFERRFGAGKLESGAELVSIATGLALLGGEDDLDDWSERASG"
     gene            complement(3428..>3807)
                     /locus_tag="PLO65_12155"
     CDS             complement(3428..>3807)
                     /locus_tag="PLO65_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29044.1"
                     /translation="QGQALRACRSATRSALEQPVPSGDDLAVRLRAGWAMTDGSDQET
                     SLEHRAARRLNVTMVALLALAGTVIGLWVFLSELPAIRGIFDALPARQQETLFRAGFG
                     LLITMAGLTHLLPIVGKVFNRGD"
BASE COUNT          616 a         1286 c         1307 g          598 t
ORIGIN      
        1 gatggaaggc aaggcggtgc tgttcaagcg cttcgccgac gtcgacgcga tcgacgtcga
       61 gctcagcacc gaggatcccg acgagatcat taccgtggtc aagaccatcg gggcgacgtt
      121 cggcggcatc aacctcgagg acatcaagag ccccgagtgc ttcatcatcg agcagcgctg
      181 ccaggagctg ctcgacatcc cggtgttcca tgacgaccag catggcacgg cgatcatctg
      241 cgccgccggc ctgatcaacg cctgctatat caccggcaag aagatcgagg acgtgaaggt
      301 cgtgctgaac ggccccggcg cggccggcat cgcctcgctc gaactgatca aggcgatggg
      361 cgtgcgtccc gagaactgca tcgcctgtga ctccaagggc gtgctctacc agggccgcac
      421 cgacggcatg aaccagtgga agagcgccca cgcggtcgat accgacaagc gcaccctggc
      481 ggaggccgtg gcgggggcgg acgtgctgct gggcctgtcg gccaagggcg ccttcacccg
      541 ggagatgatc gccagcatgg cgcccaaccc ggtgatcttc gccatggcca accccgaccc
      601 ggagatcacc cccgaagagg tgcacgcggt ccgcagcgac gccatcatgg gcaccggccg
      661 cagcgactat cccaaccagg tcaacaacgt gctgggcttc ccctacatct tccgcggggc
      721 gctcgacgtg cgggcccggc gggtcaatca cgagatgaag atcgcctgcg ccaacgccct
      781 ggccatgctc gcccgggagg acgtgccgga cgaggtggcc gcggcctatc acggccggca
      841 gctgaagttc ggcccgcagt acatcatccc ctcggccttc gacccccggc tcatctggta
      901 catcccgccg ttcgtcgccc aggcggcgat ggataccggc gtggcccgcc ggccgatcgc
      961 cgacatggac gcctaccgcg cctcgctggc ccagcgcctc gacccgacgg cggccttcct
     1021 gcagaagatc tccggggcgg tgatgaacgg ccccaagaag cgcatcgtgt tcgccgaggg
     1081 cgaggagccc agcgtgatcc gcgccgccta cgccttccag gcccagggct atggcgaggc
     1141 catcctctgt ggtcgcgagg agctggtgaa ggagaacatg aagctggccg gcctcgatcc
     1201 ggacgaggtc cagctgcaga tcgtcaacgc ccggctgtcg gaccgcaatc ccgactatgt
     1261 cgagcacctg taccagcgcc tgcagcgcga gggttatctc aagcgcgacg tgcagcggct
     1321 gatcaaccag gaccgcaaca gcttcgccgc ctcgatggtc gccctgggcg acgccgacgc
     1381 gatggtcacc ggcgtgaccc gcagctacga tcaggtgctg gaggaggtcc tgcgggtcat
     1441 ggatccggcg cccggcggcc gggtgatggg catgtcgatc gtgctggcca agggccacac
     1501 ggtgttcatc gccgacacca acgtcaccga gatgcccgag gccgacgagc tggtcgagat
     1561 cgcctgcgag gcggcccgcg ccgtgcggac gctcggcttc acgccgcgcg tggccttcat
     1621 gagctactcg accttcggca atccgatggg cctgcgctcg gagaaggtcc gcgaggccgt
     1681 ggccatgctc gacgagatgg acggcgtcga cttcgagtac gagggcgaga tgccgcccga
     1741 gctggccctt gagccggagt cgcgcgacaa tttcccgttc atgcgcctga ccggcccggc
     1801 caatgtgctg atcatgccgg cgatccactc ggcctcgatc tcgaccaagc tggtccaggc
     1861 cctgggcggg gcgaccgtgc tgggtccgat cctgctgggc ctgaacaagt cggtgcagat
     1921 cgcgccgctg tcggcctcgg tgtcgaagat cctcaacatg gctctgatgg cggcctacga
     1981 ccagccgctc gagccggcgg cggagtaggg ccatggcttc atgagggtct gcgccgtgac
     2041 gagtttcatt ccggcctgac gtcgcccttg cgcagggatg gcgtccgccg ccatccatac
     2101 tgcgaaggga atttcccatg acgcgcgttt ccgacgccgc gatcggtgtc gatttcggca
     2161 cgaccaatac tgtgctcgcc tttgaccagg ggggcggcca ggccggcctc gccgggttca
     2221 gccacgatgg gcagccgttc aacgccttcc gctcggcgct cagcttccac gctccggccg
     2281 acgatccggc cggccgggtg gtcgaggccg ggccctgggc gatcgacgcc tatgtcgagg
     2341 agccgctcga gacgcggttc atccagtcgt tcaagagcta cgccgccagc ccgctgttca
     2401 ccgacaccga gatcctgcgc cggcggttca gtttcgagga cctgctcagc gccttcctgc
     2461 tgaaggtgcg tgaacacggc ggcgccgatc tcgccgatct gcccaagccg gtgattgtcg
     2521 gccggccggt ggccttcgcg gggctgcggc ccgacccggc cctggccctg acgcgctacg
     2581 agacggcgtt ccggcggctg ggcttcaccg acatccgcta tgtctacgag ccggtcgccg
     2641 cggccttctt cttcgcccgg cggctggagg gcgacgccac cgtgctggtc ggcgacttcg
     2701 gcggcggcac cagcgacttc tcgctggtcc ggttcacgca caagggcggc cgggtgcggt
     2761 cacaggccct gtccaacgcc ggggtcggcg tggcgggcga cgccttcgac taccggatca
     2821 tcgaccacct ggtgtcgccg aaactgggca agggcagcct gtacaaggcc ttcgacaacg
     2881 tcattccgtt gcccaaccgc tacttcaccg ccttcgcccg ctgggagcag ctggcgctgc
     2941 tgcgggcgag ccgggacatg cgcgagatcc gccggctggt gcgcgacgcg gtcgagccgg
     3001 agaagctcga gcggctggtc gaggtgctcg acgacaacca cggctaccgc ctctaccagt
     3061 cggtctcgcg gctgaaggag gcgctgtcgg gggccgagga ggcggtgttc agcttcaagg
     3121 gcgggtcggt ggagatcgag gggccggtga agcgggccag cttcgaccgc tggatccagc
     3181 cggaactgac ggccatcgaa ggcgcggtgg atcaggcgct ggcgcgggcc gggctgaccg
     3241 aggccggcgt cgaccgggtg ttcctcaccg gcgggtcgag cttcgtcccg gccgtgcggg
     3301 cgatcttcga gcgccggttc ggggcgggca agctggagag cggggccgag ctggtgtcga
     3361 tcgccaccgg cctggcgctg ctcgggggcg aggacgatct ggacgactgg agcgagcggg
     3421 cctctggcta gtccccgcga ttgaagacct ttccgacgat tggcaacaga tgggtgaggc
     3481 cggccatagt gatcaacaga ccgaagccgg cccggaacaa ggtctcttgt tgccgggcgg
     3541 gaagagcatc gaaaattccg cggattgccg gcagctcaga caggaaaacc cacagaccga
     3601 tcaccgttcc tgccagcgcg agcagagcga ccatggtgac gttgagcctg cgcgctgccc
     3661 gatgctcgag cgatgtctct tgatccgatc cgtcggtcat cgcccacccc gctcttaatc
     3721 tcacggcgag atcatcgccg gacgggacag gttgctcaag ggcgcttcgc gtcgcggagc
     3781 gacaggcccg cagggcctgc ccttgac
//
LOCUS       DAORNX010000296         3796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18128_length_3796_cov_5.272387, whole genome shotgun sequence.
ACCESSION   DAORNX010000296 DAORNX010000000
VERSION     DAORNX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18128_length_3796_cov_5.272387"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>254
                     /locus_tag="PLO65_12160"
     CDS             19..>254
                     /locus_tag="PLO65_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HRD29045.1"
                     /translation="MTGVPAVAPANAAIAPGPGYPYGPPPGTVDTERYPGGQSNPVRR
                     VADDPVSTFSIDVDTAAYANVRRFLNGGRLPPRDA"
     assembly_gap    255..388
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..1521
                     /locus_tag="PLO65_12165"
     CDS             <389..1521
                     /locus_tag="PLO65_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRD29046.1"
                     /translation="GYDLPATDQPPLNLVFLVDTSGSMFSPDKLDLAKKAMNLLVDQL
                     RPGDRVAIVAYAGSAGAVLEPTGGRSKLKIRCALEALQAGGSTAGGQGLALAYALAQE
                     NFDPKAVNRVVLMTDGDFNVGIADPSRLKDFVSDKRKTGVYLSVYGFGRGNYNDTMMQ
                     ALAQNGNGTAAYIDTFEEARKLFRDDFSGSLFPIADDVKIQIEFNPAQVSEYRLIGYE
                     TRMLAREDFNNDRVDAGEVGSGVAVTALYEITPAGARPSADPLRYGADAPAAAPSGEM
                     AFLKVRWKAPGGAESRLIQQPIPSRDTAADLASAPEATRWAVAVAAFGQKLRGDPWLE
                     DGYGWDQILDTAQHARGDDLFGLRAEFVKLVRAARDARRVNE"
     gene            1564..3369
                     /locus_tag="PLO65_12170"
     CDS             1564..3369
                     /locus_tag="PLO65_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRD29047.1"
                     /translation="MPLKIEDYALIGDCEACALVGLDGSIDWLCWPRFDSDACFAALL
                     GTPDNGRWLIAPTDAAATVTRSYRGDTLILETRFETAGGVATVIDFMPPRGKNPELVR
                     IVRGDRGRVAFRSELTLRFGYGAVTPWMTRTDGGAHRAIAGPDMVILRTPVGIRGEDL
                     HSLGEFVVAEGESTPFTLSWAPSHTPPPQAISPRQALAETEQFWADWSARAEVKGRWS
                     EAARRSLITLKALTHGPTGGVIAAATTSLPEQIGGPRNWDYRYCWIRDATLTLLALMN
                     AGYYDEAQAWRDWLLRAVAGSPEDMHIMYGVAGERRLSEWELDWLPGYEGSRPVRVGN
                     AAHAQFQLDVYGELADSLHQARRGGLTGNEAAWAVERALTDHVCRVWDQPDDGIWEDR
                     ADRKHYTFSKVMAWVAVDRAVKAVEHYGLPGDADRWRAVREAIREHILERGFDPVRNT
                     FVRDFGGRTLDASLLLLAELGFVAPDDPRFVGTVAAVEQDLLRDGFVMRYDTECIDDG
                     LPPGEGAFLACSFWLVNAYVLLGRKADAVALFERLLALRNDVGLLAEEYDPVAGRQVG
                     NFPQAFSHIGLINAAFNLGRGERPSEQRAELQASE"
     gene            complement(3436..>3796)
                     /locus_tag="PLO65_12175"
     CDS             complement(3436..>3796)
                     /locus_tag="PLO65_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29048.1"
                     /translation="TGPPDAGRPAASAARRMLCHACGAVREQAQAPLGVIIDPFMGLP
                     PRLKAVTRHGELVAWNEAHLDYLERYLSGRLRIETVQPGGVRNASVISRLPAWAKAAG
                     NRAEVLRAIARLRRDKL"
BASE COUNT          529 a         1329 c         1288 g          516 t
ORIGIN      
        1 cccccgccac ccccgcccat gaccggcgtg cccgccgtgg ctccggccaa cgcggcgatc
       61 gcgccgggcc ccggctatcc ctacggcccg ccccccggaa cggtcgacac cgagcgctat
      121 cccggcggtc agtccaatcc ggtccggcgt gtcgccgacg acccggtctc gaccttctcg
      181 atcgacgtcg acaccgccgc ctacgccaat gtccgccgct tcctcaatgg cggccgcctt
      241 ccgccgaggg acgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ggctacgacc tgccggcgac cgaccagccg
      421 ccgctgaacc tggtcttcct cgtcgacacc tcgggctcga tgttctcgcc cgacaagctc
      481 gacctggcca agaaggcgat gaacctgctg gtcgaccagc tgaggccggg ggaccgggtg
      541 gcgatcgtcg cctacgccgg gtcggccggc gccgtgctgg agccgaccgg cggccggtcg
      601 aagctgaaga tccgctgcgc gctcgaggcc ctgcaggccg gcggctcgac cgcgggcggc
      661 cagggcctgg cgctggccta cgccctggcg caggagaact tcgacccgaa ggcggtcaac
      721 cgggtggtgc tgatgaccga tggcgacttc aacgtcggca tcgccgaccc gtcgcggctc
      781 aaggacttcg tgtccgacaa gcgcaagacc ggtgtctacc tgtcggtcta cggctttggc
      841 cgcggcaact acaacgacac gatgatgcag gccctggccc agaacggcaa cggcacggcc
      901 gcctacatcg acaccttcga ggaggcccgc aagctgttcc gcgacgactt ctccggctcg
      961 ctgttcccga tcgccgacga cgtcaagatc cagatcgagt tcaacccggc ccaggtcagt
     1021 gaataccggc tgatcggcta cgaaacgcgg atgctggcgc gggaggactt caacaatgac
     1081 cgggtggacg ccggcgaggt cggctcgggc gtcgcggtga ccgccctcta cgagatcacc
     1141 ccggccggcg cccgcccgtc cgccgacccg ctccgctatg gggctgacgc gccggcggcg
     1201 gcgccatccg gcgagatggc cttcctcaag gtgcgctgga aggcccccgg cggggccgag
     1261 tcccgcctga tccagcaacc gatcccgtcc cgcgacacgg cggccgacct ggcgtccgcg
     1321 ccggaggcga cgcgctgggc ggtggccgtc gcggccttcg gccagaaact gcgcggcgat
     1381 ccctggctgg aggatggcta cggctgggac cagatcctcg acacggcgca gcacgcgcgc
     1441 ggggacgacc tgttcggtct gcgggcggag ttcgtgaagc tggtgcgggc ggcgcgggac
     1501 gcccgacggg tcaacgaata gccggcaggt gcagcttccc gccgcctgct gcattgtcac
     1561 ctgatgcccc tgaagatcga agactacgcc ctgatcggtg attgcgaggc ctgcgccctg
     1621 gtcgggctgg acggctccat cgactggctg tgctggccaa ggttcgattc cgacgcctgt
     1681 ttcgcggctc tgctcggcac gcctgacaac ggccggtggc tgatcgcccc gaccgacgcg
     1741 gcggcgacgg tgacccgcag ctaccgcggc gacaccctga tccttgagac gcggttcgag
     1801 acggccggcg gcgtcgccac ggtgatcgac ttcatgccgc cgcgcggcaa gaacccggaa
     1861 ctggtccgca tcgtgcgggg cgatcgcggc cgggtcgcct tccgctcgga gctgaccctg
     1921 cgcttcggct acggggcggt cacgccgtgg atgacgcgca ccgacggcgg cgcgcaccgg
     1981 gccatcgccg gaccggacat ggtcatcctg cgcacgccgg tcggcattcg cggcgaggat
     2041 ctgcattccc tgggcgaatt cgtggtcgcg gagggagagt cgacgccctt caccctcagc
     2101 tgggcgccgt cgcacacgcc gccgccccag gccatcagcc cccgtcaggc cctggccgaa
     2161 accgaacagt tctgggccga ctggtcggcc agggccgagg tcaaggggcg ctggtcggag
     2221 gccgcgcggc gctcgctgat caccctcaag gcgctgaccc atggcccgac cggcggggtc
     2281 atcgccgcgg cgaccacctc cctgcccgag cagatcggcg ggccgcgcaa ctgggactac
     2341 cgctactgct ggatccgcga cgcgaccctg accctgctgg ccctgatgaa cgccggctac
     2401 tacgacgagg cccaggcctg gcgggactgg ctgctgcggg cggtggccgg cagtcccgag
     2461 gacatgcaca tcatgtacgg cgtcgccggc gagcggcggc tgtcggaatg ggagctggac
     2521 tggctgccgg gctacgaggg gtcccggccg gtgcgggtcg gcaacgccgc ccacgcgcag
     2581 ttccagctcg atgtgtacgg cgagctggcc gacagcctgc accaggcccg ccgcggcggc
     2641 ctgaccggca acgaggcggc ctgggccgtc gagcgggccc tgacggacca cgtctgccgc
     2701 gtctgggacc agcccgacga cggcatctgg gaggaccgcg cggaccgcaa gcactacacc
     2761 ttctcgaagg tgatggcctg ggtcgcggtc gatcgcgccg tgaaggcggt cgagcattac
     2821 ggcctgcccg gcgacgccga ccgctggcgc gccgtgcgcg aagcgatccg ggagcacatc
     2881 ctcgagcgcg gcttcgatcc ggtgcgcaat acgttcgtgc gcgacttcgg cggccggacg
     2941 ctggacgcca gcctgctgct gctggccgag ctgggcttcg tcgcgcccga cgatccccgt
     3001 ttcgtcggca cggtggcggc ggtcgagcag gacctgctgc gcgacggctt cgtcatgcgc
     3061 tacgacaccg aatgcatcga cgatggcctg ccgcccggcg agggggcctt cctcgcctgc
     3121 agcttctggc tggtgaacgc ctatgtcctg ctcggacgca aggccgacgc cgtggcgctg
     3181 ttcgagcggc tgctggccct gcgcaatgat gtgggcctgc tggccgagga gtacgacccg
     3241 gtcgcgggcc gacaggtcgg caacttcccg caggccttca gccacatcgg cctgatcaac
     3301 gcggccttca atctcggccg gggcgagcgg cccagcgaac agcgggccga actgcaggcg
     3361 tcggaatagc gggcggccgt atgcctgact cggcgtcccg cgccccgtcc aaccgggttc
     3421 cggcctggaa cgcggtcaca gcttgtcgcg acgcaggcgc gcgatggccc tgagaacctc
     3481 cgcccggttc ccggcggcct tcgcccaggc gggcaggcgc gagatcacgg aggcgttgcg
     3541 aaccccgccg ggctgaaccg tctcgatacg cagtcgacct gacaggtagc gttccaggta
     3601 gtcgaggtgg gcctcgttcc aggcgaccag ttcgccgtgg cgcgtcaccg ccttcagacg
     3661 gggcggcaag cccatgaacg gatcgatgat cacgcccaga ggggcctgcg cctgctcgcg
     3721 caccgcgccg caggcatggc acagcattcg ccgcgcagcg gacgcggccg gccgaccggc
     3781 gtccggcggc ccggtc
//
LOCUS       DAORNX010000297         3793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18151_length_3793_cov_9.490637, whole genome shotgun sequence.
ACCESSION   DAORNX010000297 DAORNX010000000
VERSION     DAORNX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18151_length_3793_cov_9.490637"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PLO65_12180"
     CDS             <1..1273
                     /locus_tag="PLO65_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29049.1"
                     /translation="ALVQLMTQGGGQAVKTRLADLVAAARGKPTVEKTGLDETFEMIR
                     DQFHAFAEEKVVPFAHGWHLRDELIPMELVRELGDLGVFGLTIPEEYDGAGLGKTAMC
                     VVSEELSRAWIGVGSLATRSEIAAELILTGGTADQKTEWLPKIASAEILPTAVFTEPN
                     TGSDLGSLRTRAVLHGDRYVVTGNKTWITHAARADVMTLLVRTDPNTTDYRGLSMLLA
                     PKLRGDEAGPFPTEGMSGGEIGVIGYRGMKEYEIGFDGFTVPAANLLGGVEGQGFKQL
                     MATFESARIQTAARAVGVAQRALELGLAYALDRKQFGGPIFGFPRVANKLAMMACEIM
                     AVRQLTYFAARQKDEGKRCDLEAGMAKLIAARVAWAAADNALQIHGGNGFAMEYEISR
                     VLADARILNIFEGAGEIQAQVIARRLLEDAN"
     gene            complement(1314..1916)
                     /locus_tag="PLO65_12185"
     CDS             complement(1314..1916)
                     /locus_tag="PLO65_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29050.1"
                     /translation="MAGVRETQKQTTRGKVLEAARELFNEIGYDETTIRAIAERAGVS
                     VGSVFTTFASKSDVLSQVMDDRVDALYAELDRVARHLRGSVVDRVSSIFAIHYDFECR
                     RVKLFLAHVAASFSPNLEPTTVPYGRNARFQDMLLTMMADGVERGEIRPDADLELVLD
                     TLMAAYAWNYRHAAQRGADASVLTGLMERQVALVFEGLRP"
     gene            complement(1972..2619)
                     /locus_tag="PLO65_12190"
     CDS             complement(1972..2619)
                     /locus_tag="PLO65_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29051.1"
                     /translation="MNHIPMRMNAPTLRPQKLTRRALAKQQTREKVLQAAREMFIERG
                     YEGATIRDIARAAGMSTGAVFASFTDKPELFDAILSDEFAALADPMRDAAATAPSARE
                     ALVAMFGAAYRAHFSQLPLIQAALAASWTRTPEAERLRRDSLRPLRALVLEVLERGVA
                     RDELTQKYDLRLIADVIWDLYLAGYRPAAFDGLSVDTQTDRLANRIDLVLASVKA"
     gene            complement(2616..2837)
                     /locus_tag="PLO65_12195"
     CDS             complement(2616..2837)
                     /locus_tag="PLO65_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29052.1"
                     /translation="MGMTQSAAAVQAADEDDSKAEIRQLIMDLRDAAQDGRELARRYA
                     DVLQKVVDEYCVEFEKRAEAALARLEAVR"
     gene            3146..3352
                     /locus_tag="PLO65_12200"
     CDS             3146..3352
                     /locus_tag="PLO65_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRD29053.1"
                     /translation="MDRLFDILIPVSLAAVLLALLAGLYALFRGGDFGRSYSNRLMRL
                     RVLLQFLAILVLVAALWWRTSHAG"
     gene            3357..>3793
                     /locus_tag="PLO65_12205"
     CDS             3357..>3793
                     /locus_tag="PLO65_12205"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRD29054.1"
                     /translation="MVTLNRIYTRTGDDGVTRLATGEPARKDSLRVEAYGGVDETNAC
                     LGLVRLHTAADDVLDPILGRIQNDLFDLGADLATPDRGKPVEWEPLRILQSQVDRLET
                     EIDRLNHELSDLTSFVLPGGTPAAAALHQARTVCRRAERACVAL"
BASE COUNT          595 a         1294 c         1309 g          595 t
ORIGIN      
        1 ggccctggtc cagctgatga cccagggcgg cggccaggcg gtgaagaccc gcctcgccga
       61 cctcgtcgcg gccgcgcgcg gcaagccgac ggtcgagaag accggcctgg acgagacctt
      121 cgagatgatc cgcgaccagt tccacgcctt cgccgaggag aaggtcgtgc cgttcgccca
      181 cggctggcac ctgcgcgacg aactgatccc catggagctg gtcagggagc tgggcgacct
      241 gggtgtgttc ggcctgacca ttccggaaga gtatgacggc gccggcctgg gcaagacggc
      301 catgtgcgtg gtgtccgagg agctcagccg cgcctggatc ggcgtgggct cgctggccac
      361 ccgctcggag atcgccgcgg aactgatcct gaccggcggc acggccgacc agaagaccga
      421 gtggctgccg aagatcgcca gcgccgaaat cctgccgacg gccgtgttca ccgaacctaa
      481 taccgggtcc gacctgggca gcctgcgcac gcgggccgtc ctccacggcg accggtatgt
      541 ggtcaccggg aacaagacct ggatcaccca cgcggcccgg gcggacgtga tgaccctgct
      601 ggtccgcacc gacccgaaca ccaccgacta ccgtggcctg tcgatgttgc tggcgcccaa
      661 gctgcgcggc gacgaggccg gtccgttccc gaccgagggc atgagcggcg gcgagatcgg
      721 cgtgatcggc tatcgcggga tgaaggaata cgagatcggc ttcgacggtt tcaccgtccc
      781 ggcggccaac ctgctcggcg gggtcgaggg ccagggcttc aagcagctga tggcgacgtt
      841 cgagagcgcc cgcatccaga ccgccgcccg cgcggtcggc gtggcccagc gcgcgctgga
      901 actgggactg gcctatgcgc tcgaccgcaa gcagttcggc gggccgatct tcggcttccc
      961 gcgggtggcc aacaagctgg ccatgatggc ctgcgagatc atggccgtgc gccagctgac
     1021 ctatttcgcg gcgcgccaga aggacgaggg caagcgctgc gatctcgagg ccggcatggc
     1081 caagctgatc gccgcccgcg tcgcctgggc cgccgccgac aacgcgctgc agatccacgg
     1141 cggcaacggc ttcgccatgg aatacgagat cagccgcgtg ctggccgacg cccgcatcct
     1201 gaacatcttc gagggcgccg gcgagatcca ggcccaggtc atcgcccggc gtctgctgga
     1261 agacgcgaac tgagggaagg ggtccggggg ccggccgcgc ctgcgcccgc cagctacggc
     1321 ctgaggccct cgaagaccag ggccacctga cgctccatga ggcccgtcaa cacgctggcg
     1381 tcggcgcccc gctgggcggc gtgacggtag ttccaggcat aggccgccat cagcgtgtcc
     1441 agcaccagct ccagatcggc gtcgggccgg atttcgcccc gctccacgcc gtcggccatc
     1501 atcgtcagaa gcatgtcctg gaaccgggcg ttgcgtccgt agggcaccgt cgtcggctcg
     1561 aggttcgggc tgaacgaggc ggcgacgtgg gccaggaaca gcttcacccg ccgacattcg
     1621 aagtcatagt gaatggcgaa gatcgagctg acgcgatcga cgaccgatcc gcgcaggtgg
     1681 cgggcgacgc ggtccagctc cgcgtagagg gcgtcgaccc ggtcatccat cacctggctg
     1741 agcacgtcgg atttcgaggc gaaggtggtg aacacgctgc cgaccgaaac ccccgcccgt
     1801 tcggcgatgg cgcgaatggt cgtctcgtcg tagccgattt cgttgaacag ctcgcgcgcg
     1861 gcttcgagca ccttgccgcg cgtggtctgc ttctgcgtct ctcgaacgcc tgccaaaccg
     1921 ttcccccccg ggaatggcgg cgtccgacga ggacgccgcg ccgtatcgcc gttaggcttt
     1981 cacgctcgcc aggaccagat caatgcgatt ggccagtctg tctgtctggg tgtcgacaga
     2041 caggccatcg aaggcggccg ggcggtagcc ggccagatag aggtcccaga tgacgtccgc
     2101 gatcaggcgg agatcatatt tctgcgtcag ttcgtcacgg gccacgccgc gctcgagcac
     2161 ctcgagcacc agggcgcgca gcgggcgaag cgaatcacgc cgcagccgtt cggcttccgg
     2221 cgtccgggtc caggacgcgg ccagggcggc ctgaatcagg ggaagctgcg agaagtgggc
     2281 gcggtaggcg gcgccgaaca tcgccaccag cgcctcgcgg gcggacggcg cggtggctgc
     2341 cgcgtcgcgc atcggatcgg ccagggccgc gaactcgtcg ctgaggatgg cgtcgaacag
     2401 ctcgggcttg tcggtgaagc tggcgaacac cgcgccggtc gacatgccgg cggcgcgggc
     2461 gatgtcacgg atcgtggcgc cttcgtagcc gcgctcgatg aacatctcgc gagcggcctg
     2521 caggaccttc tcgcgggtct gttgcttggc cagggccctg cgggtcagct tctgcggtcg
     2581 cagggtgggc gcgttcatac gcatgggaat gtggttcatc ggaccgcctc caaacgggcc
     2641 agcgccgctt cggcgcgctt ttcgaactcg acgcagtact cgtcgacgac cttctgcagg
     2701 acgtcggcat aacgccgcgc cagctcccgg ccgtcctgcg cggcgtcgcg cagatccatg
     2761 atcagctgcc ggatctcggc cttgctgtcg tcctcgtccg cggcctggac ggcggcggcg
     2821 ctctgggtca tgcccatgtc atctccgact cgtcctcccc gttggtggat ggtggtccgg
     2881 cgatccacgg tgaggagttg accgcgttca ttgaacatgc tcatcgagcg gtgaggggcg
     2941 ccacgtacag tgaacgatga tagcggtcaa acacttattt cgcggcggaa atcaggctgg
     3001 atgccacgaa ccggaccgat acttccgttc ctcgatcgtt ggaatgcgac atgttcgcac
     3061 gatgaacgca ctcgctgaac attggctgcg acccatcgtc gccggggcgg tggccgggcg
     3121 ccggtccgcg cgctagggtg tcgccatgga cagactgttc gacatcctca ttccggtctc
     3181 gctggccgcc gtcctgctgg ccctgctggc gggcctctac gccctgttcc ggggcggcga
     3241 cttcggccgg tcctactcga accggctgat gcgcctgcgc gtgctgctgc agtttctcgc
     3301 catcctggtc ctggtcgccg ccctgtggtg gcggaccagc cacgcgggct gaggcgatgg
     3361 tcaccctgaa tcgcatctac acgcgcacgg gggacgacgg ggtgacccgg ctggccacgg
     3421 gcgagccggc ccgcaaggac agccttcgcg tcgaggccta cggcggcgtg gacgagacca
     3481 acgcctgcct tggcctggtc cggctgcaca cggcggccga cgacgtgctc gacccgattc
     3541 tcggacgaat ccagaacgac ctgttcgatc tgggcgccga cctggccacg cccgaccgcg
     3601 gcaagcccgt cgaatgggag ccgctgcgca tcctgcagtc gcaggtcgac cgcctcgaga
     3661 cggaaatcga ccgcctgaac cacgaactgt ccgacctcac ctcgttcgtg ctgcccggag
     3721 ggacgcccgc ggcggcggcc ctgcaccagg cccgcaccgt ctgccgtcgg gccgaacgcg
     3781 cctgcgtcgc gct
//
LOCUS       DAORNX010000298         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18194_length_3786_cov_6.907263, whole genome shotgun sequence.
ACCESSION   DAORNX010000298 DAORNX010000000
VERSION     DAORNX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18194_length_3786_cov_6.907263"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLO65_12210"
     CDS             <1..951
                     /locus_tag="PLO65_12210"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRD29055.1"
                     /translation="TLTLAALSLRYVERPFRNGDFLTRRQVFAAGAGAVLLLGAACGL
                     AILGKGLPQRFDQRSLAMAESRLERSRGSYRDGTCFISYRFDAKDYRKDLCDAADPRR
                     PDWLLVGDSHAAHLWSGLVAANPDMNFMQVTSSGCEITLTRGDAERDVCDRMARLVYD
                     DLLINNRPDGVLLAGRWWPKDLPRLAATLDWLRARGIPVVLVGPIPQYSDDLTRLLIL
                     SWRFHDPDLPRKRRLDWPRDVDRDLRKLATDKGVAYVSLYDLLCDGEDCVTLVDAETP
                     LQWDYGHLTPEGSRFVADRLRARGLFPTRGPAPAPSPDRR"
     gene            complement(911..1432)
                     /locus_tag="PLO65_12215"
     CDS             complement(911..1432)
                     /locus_tag="PLO65_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD29056.1"
                     /translation="MSEITIRPCTAADADRLSIVAAATFIETYAGIVDGDDLVAHCHT
                     THAPRAYAALLSDPARRLFLATLAPGEAPVGFMLLGPPDLPVETGPHDIELTRIYALH
                     RFHGQGLGPRLMQTATDAARAAGARRLLLGVYSRNERACAFYARCGFRQIGTRLFQVG
                     VNAYLDWVLALDL"
     gene            complement(1442..2275)
                     /locus_tag="PLO65_12220"
     CDS             complement(1442..2275)
                     /locus_tag="PLO65_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD29057.1"
                     /translation="MSAAQTAALMMIASGATHAVVNAILKAGKDKMSSRALIDGFSAL
                     LVLPVAFFTPLPHGAWGWLAGSWATHLVYLVCLIKAFERADMSVAYPVSRGVAPALAA
                     VVSVSLFSEPITVLVACGIGLVSCGVMAVGLGRQVDRRALGWAAATGVTIALYTVIDA
                     EGVRAAPGALSYIVWVYLALGGGIGLLFALWRGPRFLLAAREAWKPGLAAGALSIVTY
                     GLALWAYRLGDTPRLAALRETSILFGVAIAVVFLGERLTPGRVAGTLAIAGGAALLIA
                     S"
     gene            2410..2949
                     /locus_tag="PLO65_12225"
     CDS             2410..2949
                     /locus_tag="PLO65_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29058.1"
                     /translation="MVSMIICVAALFSGLLFLACAWTTRAGYWRTAAALMGGLAAAAL
                     RFGVDMAAFHLDLWTFGETAYAPVVTYVPVMFWFGAGLGLVGWRMIRNWGAVGELAFF
                     AGFVLLGLGRDLLLATGNSALSFGQGPLPLVAAALSWLAMAILVQMAMQLLVGSVDSD
                     DLAPERIPQIEDDASVGWS"
     gene            3105..3578
                     /gene="greA"
                     /locus_tag="PLO65_12230"
     CDS             3105..3578
                     /gene="greA"
                     /locus_tag="PLO65_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRD29059.1"
                     /translation="MEKVPMTAGGYTALDEELKRLKTLERPSVIAAISEARAHGDLSE
                     NAEYHAAKERQGWIEGRIAEIEDKIARAQIIDVSKLSGNQVKFGATVTVVDEDTEDEG
                     RYQIVGDHEADVKQGRISLSSPLSRAMIGKEVGEVVEVNTPGGVKAYEILKVEWV"
BASE COUNT          584 a         1354 c         1300 g          548 t
ORIGIN      
        1 acgctcacgc tggccgccct gtcgctgcgc tacgtcgagc ggccgttccg caacggcgac
       61 ttcctgaccc gccgacaggt gttcgcggcc ggggccggcg cggtcctgct gttgggcgcc
      121 gcctgcggcc tggcgatcct cggcaagggc ctgccgcagc ggttcgacca gcgctccctg
      181 gccatggccg aatcgcgcct cgaacggtcg cgcggcagtt atcgcgacgg gacctgcttc
      241 atctcctacc gcttcgacgc aaaggattac cggaaggacc tgtgcgacgc cgccgacccg
      301 cggcggcccg actggcttct ggtcggagac agtcacgcgg ctcacctgtg gtcagggctg
      361 gtggccgcca accccgacat gaacttcatg caggtgacct cctccggctg cgagatcacc
      421 ctgaccaggg gcgacgccga gcgggatgtc tgcgaccgca tggccaggct cgtctatgac
      481 gacctgctga tcaacaatcg gcccgacggg gtgctgctgg ccggccgctg gtggcccaag
      541 gacctgccga ggctggccgc gacgctcgac tggcttcggg cccgggggat cccggtcgtc
      601 ctcgtggggc cgatcccgca gtattccgac gacctgacgc ggctgctgat cctgtcctgg
      661 cgcttccacg acccggacct gccccgcaaa cggcgtctgg actggccccg ggacgtcgac
      721 cgcgacctgc gcaagctcgc gaccgacaag ggcgtggcct atgtctcgct gtacgacctg
      781 ctctgcgacg gcgaagactg cgtgacgctg gtcgacgcgg agacgccgct gcagtgggac
      841 tacggccacc tgacgccgga gggctcgcgc ttcgtcgccg accggctacg cgctcgcggc
      901 ctgttcccga ctagaggtcc agcgccagca cccagtccag ataggcgttg acgccgacct
      961 ggaacaggcg ggttccgatc tgccggaaac cgcacctggc gtagaaggcg cacgctcgct
     1021 cgttccggct gtagacgccc agcaacagcc gccttgcccc cgccgcccgg gccgcatcgg
     1081 tcgccgtctg catcagccgc ggccccagac cctggccgtg aaaccggtgc agggcgtaaa
     1141 tccgtgtcag ctcgatatcg tgcggaccgg tctcgaccgg caggtccggt gggcccagca
     1201 gcatgaagcc gaccggcgcc tcgccgggcg ccagggtcgc caggaacagc cgacgcgccg
     1261 ggtccgacag caacgcggcg taggcccgcg gcgcatgggt tgtgtggcag tgggcgacca
     1321 ggtcatcgcc gtcgacgatg ccggcgtagg tctcgatgaa ggtcgccgcc gccacgatcg
     1381 acagccggtc ggcatcggcg gcggtgcagg ggcggatggt gatttcgctc atggctgcct
     1441 tctagctggc gatcagcagg gccgcacccc cggcaatggc cagggttccg gccacccgtc
     1501 ccggcgtcag ccgctcgccc aggaagacca ccgcgatggc cacgccgaac aggatcgagg
     1561 tctcccggag cgccgccagc cggggcgtgt cgccgagccg gtaggcccac agggccaggc
     1621 cgtaggtgac gatgctcagc gcgccggccg ccagccccgg cttccacgcc tcgcgggccg
     1681 ccagcaggaa gcgcggtcca cgccacagcg cgaacagcag cccgatgccg ccgcccagcg
     1741 ccagatagac ccagacgatg tagctcaggg cgcccggcgc ggcccgcacc ccctcggcgt
     1801 cgatcaccgt gtagagggcg atggtcaccc ccgtcgccgc ggcccagccc agggccctgc
     1861 gatcgacctg ccgccccagc cccacggcca tcacgccgca ggacaccagg ccgatgccac
     1921 aggccaccag cacggtgatc ggctcggaga acaggcttac cgacacgacc gccgccaggg
     1981 cgggggcgac gccgcgcgag accggatagg cgaccgacat gtcggcccgc tcgaacgcct
     2041 tgatcaggca gacgaggtag accaggtgcg tggcccagct gccggccagc caaccccagg
     2101 cgccatgcgg caggggtgtg aagaaggcca ccggcagcac cagcagggcc gaaaagccgt
     2161 cgatcagcgc ccggctcgac atcttgtcct tgccggcctt gaggatcgcg ttgaccaccg
     2221 cgtgggtcgc gcccgaggcg atcatcatca gcgcggcggt ctgggcggcg gacatggccc
     2281 agtcgtgaca ggcttacgcg gcggcgcgca accgcggcct gaacggttaa ccacgatgct
     2341 ccccccacgg atgaagcggg gtgcggcgcg ccggatcccg acctagcgtc accccgaggg
     2401 gatttcgcca tggtctcgat gatcatctgt gtcgcggcgc tgttctccgg cctgctgttc
     2461 ctggcctgcg cctggaccac gcgcgccggc tactggcgca ccgcggcggc cctgatgggc
     2521 gggctggcgg ccgcggccct gcggttcggc gtcgacatgg ccgccttcca cctcgacctg
     2581 tggaccttcg gcgagacggc ctacgccccg gtcgtgacct atgtgccggt catgttctgg
     2641 ttcggggcgg gcctggggct ggtcggctgg cggatgatcc gcaactgggg ggcggtgggc
     2701 gagctggcct tcttcgccgg cttcgtcctg ctgggcctgg gccgcgacct gctgctggcc
     2761 acgggcaaca gcgccctgag tttcggccag ggcccgctgc cgctggtcgc cgcggccttg
     2821 tcctggctgg cgatggccat cctcgtgcag atggcgatgc agctgctggt cgggtcggtc
     2881 gactccgacg atctggcgcc cgaacgcatc ccgcagatcg aggacgacgc aagcgtgggc
     2941 tggtcctgac atccgtccgc gtcgccgcgg ccggcccgtt tccgctgcat cgcagcatca
     3001 gacttgtatt ttttggcctt ccggcctagt cttgacgccc ccggacgtct gcgcccgcgg
     3061 ccggctgtca acacctccca gggcgaaccc gagctaatct ccgaatggaa aaagtgccga
     3121 tgaccgccgg gggctacacc gccctcgacg aagaactgaa gcgtctcaag acgctggagc
     3181 ggccgagcgt gatcgcggcc atctccgagg cgcgcgcgca tggcgatctc agcgaaaacg
     3241 ccgagtacca cgcggccaag gaacgccagg gctggatcga gggccgcatc gccgagatcg
     3301 aggacaagat cgcgcgggcg cagatcatcg atgtctccaa gctgtcgggc aaccaggtca
     3361 agttcggcgc cacggtcacg gtggtcgacg aggacaccga ggacgagggc cgctaccaga
     3421 tcgtcggcga ccacgaggcc gacgtgaaac aggggcgcat ctcgctgtcc tcgccgctgt
     3481 cgcgcgcgat gatcggcaag gaagtgggcg aagtcgtcga ggtcaacacc cccggcggcg
     3541 tcaaggccta cgaaatcctc aaggtcgagt gggtctaggg cgaacccctt cgcccgccat
     3601 ctttccaccg tcatcctcgg gcttgtcccg aggacccaag cgcacacacg tctgacggtg
     3661 ttcatgggtg gtcgggacag gcccgaccat gacgggaatt gagcgccggt ggccaagaag
     3721 ccctcccctg cccccctgac gatctgggcc gtgtccgacg gacgggccgg gatcgaggcc
     3781 caggcc
//
LOCUS       DAORNX010000299         3768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18290_length_3768_cov_8.226771, whole genome shotgun sequence.
ACCESSION   DAORNX010000299 DAORNX010000000
VERSION     DAORNX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18290_length_3768_cov_8.226771"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLO65_12235"
     CDS             complement(<1..362)
                     /locus_tag="PLO65_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRD29060.1"
                     /translation="MRYLPLTPDDRVQMLGDIGVDSIDALFVDVPAAARRAGFVDLPH
                     VAGELEVERELATLAGQNVAAGSVPFFCGAGAYRHHVPASVDHLIQRSEFLTSYTPYQ
                     PEIAQGTLQVLFEFQTQVA"
     gene            complement(362..922)
                     /locus_tag="PLO65_12240"
     CDS             complement(362..922)
                     /locus_tag="PLO65_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29061.1"
                     /translation="MSLEVFLHPSTTSPSPAVFQAAMDRALATIGATRAEDGEGVALA
                     DGSSFELYLDDHDDIALATLSIVNDAVVDAVYVLAAETRSFLMSAGFVWRLPETGDVV
                     PALAMGFPQATLVNDRLDLRDMLDSAVLAAEGPVEDDDRQPFSDEEIEAAALAAGPPP
                     EADPIVSTLAKPFLQRLSDALFGKSI"
     gene            complement(928..1293)
                     /gene="gcvH"
                     /locus_tag="PLO65_12245"
     CDS             complement(928..1293)
                     /gene="gcvH"
                     /locus_tag="PLO65_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRD29062.1"
                     /translation="MRFTKDHEWVELAGDIATVGITAYAAEQLGDVVFVEVPDVGKTL
                     SQGDGMAVVESVKAASDVYAPVSGEVVEANAELGDAPETVNAAPEAGGWFAKIKVSDP
                     SQVEALMDRAAYENYLGTL"
     gene            complement(1306..2418)
                     /gene="gcvT"
                     /locus_tag="PLO65_12250"
     CDS             complement(1306..2418)
                     /gene="gcvT"
                     /locus_tag="PLO65_12250"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRD29063.1"
                     /translation="MSDDDLKKTPLYEAHVAAGARMVPFAGYAMPVQYRDGVLKEHLW
                     TRESAGLFDVSHMGQARLRGVSPLSAFEEVTPGDFIGLAPGKQRYSVLLNRKGGIIDD
                     LMASRPDDDGLFVIVNGACKDGDFAFMNDELAPQVSIDRLEDRALLALQGPKAVDVLK
                     AHIPECAGKVFMDSWATTGFGVDVLISRSGYTGEDGYEISVPGDAATRIWNTLLEDER
                     VRPIGLGARDSLRLEAGLPLYGHDVDETVSPVEAALTFAVSKKRREAQDFPGAARIVR
                     ELAEGPSRVRVGLKVLGGPPAREGAEIADTNGKVVGVVTSGGFAPSLGCPIAMGFVPP
                     ALSAVGTKLNVIVRGRAGAAEVVAMPFVPNRYVRKV"
     regulatory      complement(2580..2669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3010..3312)
                     /locus_tag="PLO65_12255"
     CDS             complement(3010..3312)
                     /locus_tag="PLO65_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29064.1"
                     /translation="MAIGARKPPLLLRSAPRFAEVYWVDFSSSNVLPEFDDVHPGIVI
                     RSGQKLDRPHIIVPMTTVDQTGNVFAYQPSVNPNPRRLTSRPGLSAIISTPLPRNV"
     gene            complement(3385..>3768)
                     /locus_tag="PLO65_12260"
     CDS             complement(3385..>3768)
                     /locus_tag="PLO65_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRD29065.1"
                     /translation="VFARRRLLDRHLGALGLVAGRSDDAIAQRLPLLVDDLQRRERHR
                     HRLLPTCKRQGAQVRAALVDYGRGLRKAKGDAADLAQARDLSAILLDPDPARLALLAD
                     SLKTAPRTPPGPPIGEDGWFGDLRL"
BASE COUNT          620 a         1220 c         1338 g          590 t
ORIGIN      
        1 gcgacctggg tctggaactc gaacagcacc tgcagcgtgc cctgggcgat ctcgggctgg
       61 tagggcgtgt agctggtcag gaactccgac cgctggatca ggtgatccac actcgccggc
      121 acatggtggc gataggcgcc cgcgccgcag aagaacggga ccgatccggc ggccacgttc
      181 tggccggcca gggtggccag ctcgcgctcg acctccagct cgccggcgac atgtggcagg
      241 tcgacgaacc ccgcgcggcg ggcggcggcg ggcacgtcga cgaacagggc gtcgatcgag
      301 tccacgccga tgtcgcccag catctggacg cggtcatcag gggtcagggg aaggtagcgc
      361 atcagatcga tttcccgaaa agggcgtcgg acagtcgttg gaggaagggc ttggcaagag
      421 tggagacaat cggatcggcc tcgggcggtg gacccgccgc gagcgctgcg gcctcgatct
      481 cctcgtcaga gaatggctgc ctgtcatcgt cctcgacagg tccttcggcg gccaggactg
      541 cgctgtcgag catgtcccga aggtcgagac ggtcattaac gagcgtggcc tgaggaaagc
      601 ccatggcgag cgctggaaca acatcgccgg tttccggaag gcgccagacg aatcctgcgg
      661 acatcaagaa gctgcgggtc tccgccgcca ggacatagac ggcgtcgaca acggcatcgt
      721 tgacgatcga aagggtcgcg agcgcgatgt cgtcgtgatc gtccagataa agttcgaagc
      781 ttgagccatc ggccagcgcc acgccctcgc cgtcttcagc gcgcgtcgcg ccgatggtcg
      841 ccaacgccct gtccatcgcc gcttggaaga cggcgggcga tgggctggtc gtcgaggggt
      901 ggagaaagac ctcgaggctc atgtggccta gagcgtcccg agatagtttt cgtaggccgc
      961 gcgatccatc agggcctcga cctgcgaggg gtcgctgacc ttgatcttcg cgaaccagcc
     1021 gccggcttcc ggcgcggcgt tgaccgtctc gggcgcgtca ccgagctcgg cgttggcctc
     1081 gaccacctcg cccgaaaccg gggcgtagac gtcgctggcg gccttgacgc tctcgaccac
     1141 ggccatgccg tcgccctggc tcagggtctt gccgacatcg gggacctcga cgaacaccac
     1201 gtcgcccagc tgttcggcgg cgtaggcggt gatgccgacg gtggcgatgt cgcccgccag
     1261 ctcgacccac tcgtgatcct tggtgaaacg catggctgga ccctctcaga ctttgcggac
     1321 gtagcggttt gggacgaagg gcatggcgac gacctcggcg gcccccgcgc gaccccggac
     1381 gatgacgttc agcttcgtgc ccacggccga cagggccggc gggacgaagc ccatggcgat
     1441 ggggcagccc agcgaggggg cgaacccgcc gctggtgacg acgcccacca ccttgccgtt
     1501 ggtgtcggcg atctcggcgc cttcgcgcgc cggcgggccg ccgagcacct tcaggccgac
     1561 gcgcacacgc gaggggccct cggccagttc cctgacgatg cgggcggcgc cggggaagtc
     1621 ctgggcctcg cggcgcttct tcgacacggc gaaggtcagg gccgcctcga ccgggctgac
     1681 cgtctcgtcg acgtcgtggc catacagcgg caggccagcc tcgagccgca gggagtcccg
     1741 ggcgccaagg ccgatggggc gcacccgctc gtcctccagc agggtgttcc agatgcgcgt
     1801 cgcggcgtcg ccgggcacgg aaatctcgta gccgtcctcg cccgtgtagc ccgagcggga
     1861 gatcagcacg tcgacgccga acccggtcgt cgcccagctg tccatgaaca ccttgcccgc
     1921 gcactcgggg atgtgggcct tgagcacgtc cacggccttg gggccctgca gcgccagcag
     1981 cgcgcggtcc tcgagacggt cgatgctgac ctgcggcgcc agctcgtcgt tcatgaacgc
     2041 gaagtcgccg tccttgcagg cgccgttgac gatcacgaac aggccgtcgt cgtcgggccg
     2101 cgaggccatc aggtcgtcga tgatcccgcc cttgcggttc agcagcacgg agtagcgttg
     2161 cttgcccggg gccaggccga tgaaatcgcc gggggtcacc tcctcgaacg cggacagcgg
     2221 cgagacgccg cgcagtcggg cctggcccat gtgcgacacg tcgaacagtc cggcgctctc
     2281 gcgggtccag agatgctcct tgagcacccc gtcccggtac tgcacgggca tggcatagcc
     2341 ggcgaacggc accatgcgcg cgccggccgc gacgtgcgcc tcgtaaagcg gggtcttctt
     2401 gagatcgtcg tcagacacgc ggagctccgg cggggtcgat gcggctcgcc ggaccgatgg
     2461 cctggcgatt cgcccccgct gtctcggagc ctgagagatt tcgggacggc cccttcaaga
     2521 aaggaagtcc ggcccttgct ccttcggcgc accgcggatg cggcgctttc cagcgtttta
     2581 cctgtcgcgc ggtccgtttg cctgagcgtt tccggggcgg ttgcgccttc ggcctcggga
     2641 cgaatcccga tgtctcccgc gagacggagg ttaagtgcgg gacgggaggc ggggagtcaa
     2701 ctgcggcggg ctttgacacc ggaacgcaaa cggccggacg ctctgcagca tccggccggt
     2761 tagaggcctc ctcgcggagg caagggccaa gaccccgcgg tctcaggacc gtgcatgcaa
     2821 cttaaggccg aatgcgtcga gattcaaggt catcgtgctc ggggcgggcc gggtgcgacc
     2881 gctatggcaa ccccagggag acttcaagga cgcgccggag ggccagtcgc accagctttc
     2941 cgacttctcg catgtcgtgc gtagagaggg gtgggtagac tggattattg aaccggttgc
     3001 gcatcggcct cagacgttcc gaggcaacgg tgtagagatg attgcagaca gcccaggccg
     3061 ggaggtcagg cgccttggat tggggttcac tgacggctga taggcaaaca cgttgccggt
     3121 ctgatcgacg gtggtcatcg gcacgatgat gtgaggccgg tcgagtttct ggcccgagcg
     3181 gatgacgatg ccggggtgga cgtcgtcgaa ttccggaagc acgttcgaag aggagaagtc
     3241 gacccaatag acctccgcaa accgaggtgc gctacgaagg agtagcggcg gcttccttgc
     3301 tccgatcgcc atagggctgc ctcaacacga aaggcaatca tgacgtgtct acgagccttt
     3361 cacaaccggg cagtgggaag tccgctacag ccgcaaatcc ccgaaccagc cgtcctcgcc
     3421 gatcggcggg cccggcgggg tgcggggggc ggtcttgagg ctgtcggcca gcagggcaag
     3481 acgcgccggg tccgggtcca gcaggatggc cgacaggtcg cgcgcctggg ccaggtcggc
     3541 cgcgtccccc ttcgccttgc gcaggccgcg gccgtagtcg accagggccg cgcggacctg
     3601 ggcaccctga cgtttgcagg tggggagcag gcggtgccgg tgccgttcgc gacgctgaag
     3661 gtcgtcgacg agaaggggca gccgctgcgc gattgcatcg tcgctccgac cggcaacgag
     3721 gccgagcgcg ccaaggtgcc ggtcaagcag gcggcgcctg gcgaatac
//
LOCUS       DAORNX010000300         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18326_length_3762_cov_8.334502, whole genome shotgun sequence.
ACCESSION   DAORNX010000300 DAORNX010000000
VERSION     DAORNX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18326_length_3762_cov_8.334502"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /gene="accB"
                     /locus_tag="PLO65_12265"
     CDS             <1..296
                     /gene="accB"
                     /locus_tag="PLO65_12265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRD29066.1"
                     /translation="AAPVEAAPPPAAAAAAPVHNGETVKSPMVGTIYLQPTPGADAFI
                     KVGQTVTQGQTLLIVEAMKTMNPIPAPKAGKVVEILVADAQPVEFGEPLVVIE"
     gene            306..1649
                     /gene="accC"
                     /locus_tag="PLO65_12270"
     CDS             306..1649
                     /gene="accC"
                     /locus_tag="PLO65_12270"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRD29067.1"
                     /translation="MFDKILIANRGEIALRVHRACKEMGIATVAVHSEADANGMWVRL
                     ADESVCIGPAPAAKSYLNIPSIIAACEITGAQAVHPGYGFLSENARFAEIVGAHGYTF
                     IGPKPEHIRMMGDKITAKQAVKEAGIPVVPGSEGALTTEEQAFAAADAIGFPVLIKAA
                     AGGGGRGMKVAETREDLSEAVATAQGEARAAFGDDAVYMERYLQTPRHIEIQVIADSF
                     GNVVHLGERDCSLQRRHQKVMEEAPSPALDAAARTKIGEVVVEAIRAIGYLGVGTIEF
                     LWEKGEFFFIEMNTRLQVEHPVTEAITGIDLVREQIRIAAGLPLSFSQADVVFEGHAI
                     ECRINAENARTFAPSPGAINGFHAPGGLGVRLDSALYDGYSIPPYYDSLIGKLIVHGR
                     DRAECIARTQRCLGEMVVSGVDTTIPLFQDLLQQPDIVAGAYNIHWLEQWIKAQG"
     gene            1650..2300
                     /gene="aat"
                     /locus_tag="PLO65_12275"
     CDS             1650..2300
                     /gene="aat"
                     /locus_tag="PLO65_12275"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRD29068.1"
                     /translation="MAHDGFDIDDLVACYRRGVFPMADARDDDRVFLIDPQRRGVIPL
                     DGFHVPARLARTVRSGRFQVSIDTDFAGVVEACAEARPGRPETWINHPIQHLYERLHQ
                     SGRAHSVECRLDGGLVGGLYGVALGGAFFGESMFSTARDASKVALVHLVGRLIVGGYR
                     LLDTQFMTDHLSRFGAIEISRAAYRRRLDEALEAPADFYRFVDSGPAALQAISQAS"
     gene            complement(2243..2854)
                     /locus_tag="PLO65_12280"
     CDS             complement(2243..2854)
                     /locus_tag="PLO65_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HRD29069.1"
                     /translation="MRLTLPLLAALAIAGAVQAQQTPPPVAPSATPPSTVDQPPPPET
                     EEAPPAPSASPPAAPPAPTTAEVPVTSEVKPKAQTVEKPTEPARRIRSNAAIIQAIDK
                     VTAETLRFEAPVGQPIRYKTLVFTVRACETSAPDEAAPESAAYITIESQPKSLTGRAP
                     PPPRQVFRGWMFASSPGLNPLQHPVYDAWLIACRAAGPESTKR"
     gene            complement(2862..3257)
                     /locus_tag="PLO65_12285"
     CDS             complement(2862..3257)
                     /locus_tag="PLO65_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HRD29070.1"
                     /translation="MLKAIFTWWNGATIGQKFHIGRRGVFVGQDELGNRYYEARDNRD
                     SYDNRKRRWVIYNGYAEASKVPPDWHGWLRYTFDEPPTIAPLPRRSWEKDHRPNLTGT
                     VHAWRPKGSIAQGGQRDRATGDYLPWTPE"
     gene            3450..>3762
                     /locus_tag="PLO65_12290"
     CDS             3450..>3762
                     /locus_tag="PLO65_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29071.1"
                     /translation="MRFAKRLARVAAPEVELRTVETADALTGVLAPRGWTDARVEAWL
                     DWSETVPPYDDDRDTDGAALLAGGPARFAGGLAVRGMTLGVLDRRADRLAFEAELTAA
                     MA"
BASE COUNT          566 a         1300 c         1301 g          595 t
ORIGIN      
        1 tcgccgcgcc cgtcgaggcg gccccgccgc cggcggcggc ggccgcggct ccggttcaca
       61 acggcgaaac cgtcaagtcg ccgatggtcg gcaccatcta cctgcaaccc actccgggcg
      121 ccgacgcctt catcaaggtg ggccagaccg tgacccaggg ccagaccctg ctgatcgtcg
      181 aggcgatgaa gaccatgaac ccgatcccgg ctccgaaggc cggcaaggtc gtcgagatcc
      241 tggtggcaga cgcccagccc gtcgagttcg gcgagccgct ggtcgtgatc gaataggccc
      301 ggcctgtgtt cgacaagatc ctgatcgcca accggggcga aatcgccctg cgggttcacc
      361 gcgcctgcaa ggaaatgggc atcgccaccg tggcggtcca ttcggaggct gacgccaacg
      421 gcatgtgggt gcggctggcg gacgaaagcg tctgcatcgg ccccgccccg gccgccaaga
      481 gctatctcaa catcccctcg atcatcgccg cctgcgagat caccggggcc caggccgtgc
      541 atcccggcta cggcttcctg tcggaaaacg cccgcttcgc cgagatcgtc ggcgcccacg
      601 gctacacctt catcgggccc aagcccgagc atatccgcat gatgggcgac aagatcaccg
      661 ccaagcaggc ggtcaaggag gcgggcattc cggtggtgcc gggttccgaa ggcgccctga
      721 ccaccgagga acaggccttc gccgcggccg acgcgatcgg cttcccggtg ctgatcaagg
      781 ccgccgccgg cggcggcgga cgcggcatga aggtggccga aacgcgcgag gacctgtccg
      841 aggccgtggc caccgcccag ggcgaagccc gcgcggcctt cggcgacgac gcggtctata
      901 tggagcgcta cctccagacg ccgcggcaca tcgagatcca ggtcatcgcc gacagcttcg
      961 gcaacgtcgt gcatctgggc gagcgggact gctcgctgca gcgtcgccac cagaaggtca
     1021 tggaggaggc tccctccccg gccctcgacg ccgccgcccg gacgaagatc ggcgaggtgg
     1081 tcgtcgaggc gatccgcgcc atcggctacc tcggcgtcgg cacgatcgaa ttcctgtggg
     1141 aaaagggcga gttcttcttc atcgaaatga acacccggct gcaggtcgag catccggtca
     1201 ccgaggcgat cactggcatc gatctggtgc gagaacagat ccgcatcgca gccggcctgc
     1261 ctctgagctt ctcgcaggcg gacgtggtgt tcgaggggca cgccatcgag tgccgcatca
     1321 acgccgagaa cgcccgcacc ttcgcgccct ctcccggcgc gatcaacggc ttccacgcgc
     1381 ccggcggtct gggggtgcgg ctcgattccg ccctctacga cggctattcg atcccgccct
     1441 actacgacag cctgatcggc aagctgatcg tccacggacg cgaccgcgcc gaatgcatcg
     1501 cccgcaccca gcgctgcctg ggcgagatgg tggtcagcgg cgtggacacc accattcccc
     1561 tgttccagga cctgctgcag cagccggaca ttgtcgcggg ggcctacaac atccattggc
     1621 tggagcagtg gatcaaagcg cagggctagg tggctcacga cggcttcgac attgacgacc
     1681 tggtcgcctg ttatcggcgc ggcgtctttc cgatggccga cgcgcgtgac gatgaccggg
     1741 tcttcctgat cgacccccag cggcgcggcg tgatcccgct ggacggtttc cacgtcccgg
     1801 cccggctggc ccgcaccgtg cggtcaggcc gattccaggt cagcatcgac acggatttcg
     1861 ccggcgtggt ggaggcctgc gcggaggcgc gaccgggccg ccctgaaacc tggatcaacc
     1921 atccgatcca gcacctgtac gaacgtttgc atcagtcggg acgggcccac agcgtcgaat
     1981 gccgcctcga cggcggtctc gtcggtggcc tgtatggcgt cgcactgggc ggcgccttct
     2041 tcggcgagag catgttcagc acggcccgcg acgccagcaa ggtcgcgctg gtgcatctcg
     2101 tcggacggct gatcgtcggc ggctaccggc tgctggacac ccagttcatg accgaccacc
     2161 tgtcccgctt cggcgcgatc gaaatctccc gcgcggccta tcgccggcgt ctcgacgaag
     2221 ccctcgaggc tccggccgac ttctatcgtt tcgtggactc gggccccgcc gccctgcagg
     2281 cgatcagcca ggcgtcatag accgggtgct gcaacggatt gagccccggc gaggaggcga
     2341 acatccagcc gcggaagacc tgtcgcggcg gcggcggcgc ccgccccgtc agcgacttcg
     2401 gctgggattc gatggtgatg taggctgcgg attccggcgc ggcctcgtcc ggcgcgctgg
     2461 tctcgcaggc gcggaccgtg aacaccaggg tcttgtagcg gatcggctgg ccgaccgggg
     2521 cttcgaaccg caaggtctcc gctgtgacct tgtcgatggc ctggatgatg gccgcgttcg
     2581 agcgaattcg ccgcgccggc tcggtcggct tctcgacggt ctgggccttc ggcttgacct
     2641 cgctggtgac tggcacctcg gcggtcgtcg gcgccggagg ggcggcgggt ggcgaggctg
     2701 aaggcgcggg cggcgcctct tccgtctccg gcggcggcgg ctggtcgacc gtcgatggcg
     2761 gagtcgcgga cggcgcaacc ggcggcgggg tctgttgcgc ctggaccgcg ccggcgatgg
     2821 ccagcgccgc cagcagggga agggtcagcc gcatcggcgc gctactcggg ggtccagggc
     2881 agatagtcgc cggtggcccg gtcccgctgg ccaccctggg cgatcgagcc cttcggccgc
     2941 caggcgtgga ccgtgccggt caggttgggc cgatggtcct tctcccacga ccgccttggc
     3001 aggggggcga tggtcggcgg ctcgtcgaag gtgtagcgca gccatccatg ccagtccggc
     3061 ggcactttcg aggcctcggc atagccgttg tagatcaccc agcgccgctt gcgattgtcg
     3121 tagctgtcgc ggttgtcgcg ggcctcgtag tagcggttgc ccagctcgtc ctggccgaca
     3181 aacacgccac gccgtccgat gtggaacttc tggccgatcg tggcgccgtt ccaccaggtg
     3241 aagatcgctt tgagcacgcg caaatccgct gaagacaagg tcttgaggtc ggccggacca
     3301 tagcggtcgg ggcccggccc gtccagcggc gcgccgcagg aacccaccgc aacatgttgt
     3361 ggatgggcga cgggtcgcac gccatatcta ttgcccggac cccgccccct ctctagcctt
     3421 ggacacaact gctgtggaga gtcgggtcga tgcgtttcgc caagcggctg gccagggtcg
     3481 ccgcgccgga agtcgaactc cgcacggtgg agacggccga cgccctcacc ggggtgctgg
     3541 cgccgcgcgg ctggaccgac gccagggtcg aggcctggct tgactggtcc gagaccgttc
     3601 cgccgtacga cgatgatcgt gacaccgatg gcgcggccct gctggccggc ggtcccgcgc
     3661 gtttcgccgg cggcctggcg gtgcgcggca tgacgctcgg ggtgctcgat cgtcgcgccg
     3721 acaggctggc gttcgaggcc gaactgacgg ccgccatggc cg
//
LOCUS       DAORNX010000301         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18327_length_3762_cov_7.263555, whole genome shotgun sequence.
ACCESSION   DAORNX010000301 DAORNX010000000
VERSION     DAORNX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18327_length_3762_cov_7.263555"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>339)
                     /locus_tag="PLO65_12295"
     CDS             complement(<1..>339)
                     /locus_tag="PLO65_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRD29072.1"
                     /translation="RQRRVAAAVEKQQRLLAAPPESLLDEKGLSYERDEDLVLRRQLS
                     ADGRSRAFVNDQATGVAALKELGGLLLEVHGQHETVGLLDPRTHRALLDAFGGVSTAA
                     AAGAWSAWRAA"
     assembly_gap    340..388
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<389..510)
                     /locus_tag="PLO65_12300"
     CDS             complement(<389..510)
                     /locus_tag="PLO65_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD29073.1"
                     /translation="MLIGLAIRDVVLIEALDLAIGPGLTALTGETGAGKSIILDA"
     gene            complement(541..1518)
                     /gene="bamD"
                     /locus_tag="PLO65_12305"
     CDS             complement(541..1518)
                     /gene="bamD"
                     /locus_tag="PLO65_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRD29074.1"
                     /translation="MLPRSTGRAALLILAIAVSLTAAGCAREKPKPRLAYEERPVELL
                     YSTGAFKLDQRRWSEAVAYFQEVERQHPYSEWSRRAILMTAYAHYQGNMYADAIKDSE
                     RFIQLYPGNASVVYAYYLKAICYFEQIVDVGRDQAATEQALTSLNEVIQRFPQSEYAA
                     DARLKVDMVNDQLAGKEMAIGRYYLRQGQTIAAIGRFRTVVDRYQTTTHTPEALYRLV
                     EAYLTVGLMQEATANGAVLGHNFPGDPWYSDAYALLTDKGLQPAVAPKTPGAKRSYLD
                     RLLNRGTALPPPGEQAPSASPPADAVQPTATPPATKPEKKGVLSRLFRH"
     gene            complement(1610..2506)
                     /gene="lpxC"
                     /locus_tag="PLO65_12310"
     CDS             complement(1610..2506)
                     /gene="lpxC"
                     /locus_tag="PLO65_12310"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRD29075.1"
                     /translation="MSVSAYHQHTVAGPVIFAGVGVHTGAHVRVAVRPAAPNAGITFV
                     RTDIRDGDNIIRVSAEAVGQTRLGTVIVNDAGASVSTIEHLMAAMSALAIDNAVVELD
                     GPEVPILDGSAEVFIQVLDRAGRRRQEAPRRFIEVLEPVEVIDGDKSATLLPCDSFEM
                     AFEIAFDSAPIGRQRVDLEITEQSFRDELADCRTFGFLKDVEALRAAGLARGASMENA
                     VVLDGDRVLNPEGLRRPDEFVRHKALDAVGDLYVLGAPLMARFEGLYAGHGLNNQLVR
                     ALMANPQAWRVRTFAPELAQVG"
     gene            complement(2737..>3762)
                     /locus_tag="PLO65_12315"
     CDS             complement(2737..>3762)
                     /locus_tag="PLO65_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRD29076.1"
                     /translation="NQNLFRVANERTTFAEAFGMADQVLHSGVRSITDLMVLPGLINL
                     DFADVRTVMTEMGKAMMGTGEATGEDRALMAAQNAIQNPLLDEVSLKGAKAVLVNVTG
                     GLDMTLLEVDEAANAITDQVDPEANVIFGAAFDPALEGSIRVSVVATGMDGASIAAIE
                     PARPQRVNATARPLIVEPVQPIAAEPAFEPVRAAEPVYAAPREPERIEPEPVFEARQS
                     DLGFDAPLIPTPPPVITPPTVHVAQAETPRTVIVDPLVAEEDELDAPLYADPHFEDSR
                     QQRKGFLSLFGGRPQQQQPRYEGPPTMRAAGRTGAAAAAPQLLEEAQVDDGEDLEIPS
                     FLRRLAN"
BASE COUNT          581 a         1217 c         1325 g          590 t
ORIGIN      
        1 cgccgcgcgc caggcgctcc aggcgccggc cgccgctgcc gtcgagacgc cgccgaaggc
       61 gtccagcagg gcgcgatggg tgcggggatc gagcaggccg accgtctcgt gctggccatg
      121 cacctcgagc agcaggccgc ccagctcctt gagcgcggcc acgccggtgg cctggtcgtt
      181 gacgaaggcg cggctgcggc cgtcggccga cagctggcgg cgcaggacca gatcctcgtc
      241 ccgttcatag ctgaggccct tctcgtcgag cagcgattcg ggcggcgcgg cgagcaggcg
      301 ctgctgtttc tcaaccgccg cggctacgcg ccgctgacgn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gtcgaggatg atcgacttgc cggcaccggt
      421 ctcgccggtc agggcggtca gccccggtcc gatggcgaga tccagcgcct cgatcagcac
      481 gacgtcgcga atggccagcc cgatcagcat gaccggcagt ctagcctaat tcggccgttg
      541 tcagtgccgg aacagccggc tcaggacgcc ctttttctca ggcttggtcg caggcggcgt
      601 cgcggtcggc tggacggcgt cggccggcgg cgaggcggaa ggcgcctgct cgcccggggg
      661 cggcaaggcc gtgccgcgat tgagcagccg gtcgaggtag ctgcgcttgg cgccgggcgt
      721 cttgggcgcc acggccggct gcagcccctt gtcggtcagc agcgcatagg cgtcgctgta
      781 ccagggatcg cccgggaagt tgtggcccag caccgcgcca ttggccgtgg cctcctgcat
      841 caggcccacg gtcaggtagg cctcgacgag acgataaagg gcctcgggcg tgtgggtcgt
      901 ggtctggtaa cggtcgacca cggtgcggaa ccggccgatg gcggcgatgg tctggccctg
      961 gcgcagataa tagcggccga tggccatttc cttgccggcg agctggtcgt tgaccatgtc
     1021 gaccttgagt cgggcgtcag ccgcgtattc gctctggggg aagcgctgga tcacctcgtt
     1081 cagcgaggtc agggcctgtt cggtggcggc ctggtcgcga cccacatcga cgatctgctc
     1141 gaaatagcag atggccttca gatagtaggc gtaaaccacc gaggcgttgc ccggatagag
     1201 ctggatgaac cgctccgagt ccttgatcgc gtcggcgtac atgttgccct ggtagtgggc
     1261 gtaggcggtc atcaggatcg cccggcggga ccattccgaa tagggatgct ggcgctccac
     1321 ctcctggaag taggcgaccg cctccgacca gcggcgctgg tcgagcttga aggcgcccgt
     1381 cgaatacagg agctcgacgg gccgttcctc gtaagccagc cgcggcttgg gcttttcgcg
     1441 cgcgcagccg gccgcggtca gcgaaacggc gatggcgagg atgagaaggg ccgcccggcc
     1501 agtcgatcta ggaagcacgt accctcacct cgaaccagtt cgcgcgcccc ggagcgcagt
     1561 cccggcttct acaccacaca aaggcgggcg caaatgcggc ggaatccgtt caaccgacct
     1621 gggccagttc cggcgcgaag gtgcggacgc gccaggcttg cggattggcc atcagcgcgc
     1681 gcaccagctg gttgttcagg ccgtggccgg cgtagagccc ttcgaagcgg gccatcagcg
     1741 gcgcgcccag gacatagagg tcgccgacgg cgtccagggc cttgtggcgc acgaactcgt
     1801 ccggacggcg caggccttcc gggttcaaca cgcggtcgcc gtcgagcacc acggcgttct
     1861 ccatcgacgc gccacgcgcc aggccggccg cgcgcagggc ttcgacatcc ttgaggaagc
     1921 cgaaggtgcg gcagtccgcc agttcatcac ggaatgactg ctcggtgatc tccagatcga
     1981 cgcgctggcg gccgatcggg gccgagtcga aggcgatctc gaacgccatc tcgaagctgt
     2041 cgcagggcag cagggtcgcg gacttgtcgc cgtcgatcac ctcgaccggc tccagcacct
     2101 cgatgaagcg ccggggcgcc tcctggcggc gacgcccggc gcgatccagg acctggatga
     2161 acacctcggc cgacccgtcg agaattggca cctcgggtcc gtccagctcg acgaccgcgt
     2221 tgtcgatcgc cagggccgac atggccgcca tgaggtgttc gatcgtcgag acgctggccc
     2281 cggcgtcgtt gacgatcacc gtgcccagac gcgtctggcc gacggcctcg gcggaaaccc
     2341 ggatgatgtt gtcgccgtcc ctgatgtcgg tgcgcacgaa ggtgatgccg gcgttcggcg
     2401 cggcgggacg cacggcgacc cggacatggg cgcccgtgtg aacgcccacg cccgcgaaga
     2461 tcacggggcc ggcaacggta tgctgatggt acgccgacac ggacacgaga gaccctttga
     2521 caatctgctg agggtcacct gcgaccgctc aacgccccac gtgccattta agggcgatgc
     2581 tgcaacgcgg caaaacaatc cctgtttcgg attgttaccc ttggaaacta ttgaaattat
     2641 gaggaaaaat gaaaaatccc ggccagactg aaacagtccg gccgggatcg gtgatgctgg
     2701 cggttgagcc ggtcgcccgg cttttcccgc gtggggctag ttggccagtc gacgcaggaa
     2761 cgaggggatc tcgagatcct cgccgtcgtc gacctgggcc tcttccagca actggggcgc
     2821 ggccgcggcg gcgccggtac ggccggcggc gcgcatcgtc ggcggtccct cgtagcgcgg
     2881 ctgctgttgc tgcggccggc caccgaacag gctcaggaag cccttccgct gctggcggct
     2941 gtcctcgaaa tgggggtcgg cgtaaagcgg cgcgtccagc tcgtcctcct cggccaccag
     3001 cgggtcgacg atcaccgtac gcggggtctc cgcctgggcg acgtggacgg tcggcggcgt
     3061 gatcacgggc gggggagtcg ggatcagcgg cgcatcgaag ccgagatcgg actgacgggc
     3121 ctcgaacacg ggctcgggct cgatgcgctc gggctcgcgc ggggcggcgt agaccggctc
     3181 ggcggcgcga accggttcaa aggcgggttc cgcggcgatc ggctggaccg gctcgacgat
     3241 cagcggacgg gccgtggcgt tgacgcgctg cggccgggcc ggctcgatcg cggcgatcga
     3301 ggccccgtcc atgccggtgg cgaccaccga cacgcggatc gagccttcca gcgccggatc
     3361 gaaggccgcg ccgaagatga cgttggcttc cgggtcgacc tggtcggtga tggcgttggc
     3421 cgcctcgtcg acttccagca gggtcatgtc gaggccgccg gtcacattga ccagcaccgc
     3481 cttggcgccc ttgagcgaga cttcgtcgag cagcgggttc tggatggcgt tctgggcggc
     3541 catcagggcg cgatcctcgc cggtcgcctc gcccgtgccc atcatcgcct tgcccatctc
     3601 ggtcatgacc gtgcggacat cggcgaagtc gagattgatc aggccgggca ggaccatcag
     3661 gtcggtaatc gaacggacac ccgaatgcag cacctggtcg gccatgccga aggcctcggc
     3721 gaaggtcgtg cgctcgttgg cgacgcggaa caggttctgg tt
//
LOCUS       DAORNX010000302         3752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18399_length_3752_cov_7.760346, whole genome shotgun sequence.
ACCESSION   DAORNX010000302 DAORNX010000000
VERSION     DAORNX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18399_length_3752_cov_7.760346"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..2284
                     /locus_tag="PLO65_12320"
     CDS             488..2284
                     /locus_tag="PLO65_12320"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HRD29077.1"
                     /translation="MKLKARIAREVRFIKGLGRTLKWVKPIAPDSRDLICDDLEASVD
                     RHRDRPAITFEGKTLTYGEMDALANRYAHWAKEQGVTRGQTVALFMPNRAEYLPIWYG
                     LTKVGVATALINNNLTGAPLAHCLNISGALHCLVDAETSAAFEAVRGQLTRHVAQWTL
                     GPVSGDRRDLVTALKSCSSLRPDRETARGDLRGKDTALWIYTSGTTGLPKAARINHMR
                     AQLYMRGFAGATGAVETDRIYQVLPLYHATGGLCGMGAALLTGGSVVLRKKFSTTHFW
                     QDVHDEGCTMFVYIGELCRYLVNAPEDPLERSHRLRLAFGNGLRADVWEQLVQRFAIP
                     QVLEFYGATEGNVSMFNFDGHVGAIGRLPKWLKKRVNARLVQFDVETEQPVRGADGRC
                     IECGPGQIGECIGKIDTSDPRTNFTGYADKAATEKKVLHDVFEKGDAWFRTGDLMRQD
                     AEGYFYFVDRIGDTFRWKGENVSTGEVADRLAEAPGVLEANVYGVAVPGQDGRAGMVA
                     LTIDDDFSLTAFWDHVDRELPSYAQPLFLRLQRQIETTGTFKYRKVDLVEEGYDPARV
                     RDPLYFRNPVKKAWTRISKAAFDKINAGGFRL"
     gene            complement(2274..3509)
                     /locus_tag="PLO65_12325"
     CDS             complement(2274..3509)
                     /locus_tag="PLO65_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29078.1"
                     /translation="MTAFACLRILPPPDLHSRPGLEGLINRYACAFLEARWSWPRRFE
                     VMSLSAFLLADPRTPKLEQKILQDLAYDLQLRLFGSGGAGEVELIVFEGEPGEVARFA
                     SLDQHEVRKAVRSESLAPPLMGRLTRITVDDVRPIAVPGSPNPDLPAAPVRATADAAP
                     AAPCFQGVYFAGREAFVGCAVSNRESGFNLVDGLHPAQRDIAREHDAAIIAMAVDLLA
                     RQARFSGMFYLPVCYTSLIHRQEREDYRHWLNTLPETRRSNLAVSVYDTPRDPSFSAL
                     SQVKAFLDAYFKVVDLQTGDPDFAVERLAIKAVHSVTLVLPEASQLARIAAIRRFAER
                     IDLFRQKRIWPSISNVRNAVELETCLARRIPFISGRAVSGPLSHPVGLLSLAPDQLPL
                     DDRALHQAAMPPPARAATA"
BASE COUNT          621 a         1276 c         1306 g          549 t
ORIGIN      
        1 ctcacgcggt ccctcgcggt cgcatccccg gcgccggcga acggcgcggc ggcgtaggcg
       61 gctccgccgg cggtggcggg cgtcggttgg gcgttgtcga acagcgcccg ggcagcgtca
      121 cgatccaggg tcatggcagg cctctctgtc gatgttggtc gattgctcaa cgagactgcc
      181 ggcgcgaagg ttccacaggg accgaagggg gcgtgtgaat ttacagtgtc ttcccgctgc
      241 ttgtatcgat cggcccttcc ggtcgaccgc gaccttcaag acttcccgga tcgaggaatc
      301 gaagcgcgaa tacaggacga ttattcgcca tcatctcgcc cgggttcggc cacactcgcc
      361 gtccgcgcgg ctcgcgctgt gcgccctttg cgccacgttc accgggccgc aacgcctgaa
      421 ccctaggctg gccttgaccc tcgttcgccc gtcaggcgaa tgaggggggc cggtcggagg
      481 gcgcatcatg aagctgaagg cgcggatcgc gcgcgaggtg aggttcatca agggcctcgg
      541 tcgcacgctc aaatgggtaa agcccatcgc gcccgacagc cgcgacctga tctgcgacga
      601 cctcgaggcg tcggtcgacc ggcaccggga tcgcccggcg atcaccttcg aaggcaagac
      661 cctgacctac ggcgagatgg acgccctggc caatcgctac gcccattggg ccaaggagca
      721 gggcgtcacc cgcggccaga cggtcgccct gttcatgccc aaccgcgccg agtacctgcc
      781 gatctggtac ggcctgacca aggtcggggt ggccacggcc ctgatcaaca acaacctcac
      841 cggcgccccc ctggcccact gcctgaacat ctctggggcc ctgcactgcc tggtcgacgc
      901 cgagacctcg gccgccttcg aggccgtgcg gggccagctg acccggcatg tggcgcaatg
      961 gaccctcggc ccggtgtcgg gcgaccggcg cgacctggtc accgcgctga agagctgcag
     1021 cagcctgcga cctgaccgcg agacggcccg cggcgacctg cggggcaagg acacggcgct
     1081 gtggatctac accagcggca ccaccggcct tcccaaggcc gcccgcatca atcacatgcg
     1141 cgcccagctc tacatgcgcg gcttcgccgg cgcgacggga gcggtggaga ccgaccgcat
     1201 ctaccaggtg ctgccgctct accacgccac gggcgggctc tgcggcatgg gcgcggccct
     1261 gctgaccggc ggctcggtcg tgctgcgcaa gaagttctcg acgacccact tctggcagga
     1321 cgtgcacgac gaaggctgca cgatgttcgt ctacatcggc gagctctgcc gctacctcgt
     1381 caacgcgccc gaagacccgc ttgagcgcag ccacaggctg cggctggcct tcggcaacgg
     1441 cctgcgcgcc gatgtctggg agcagctggt ccagcggttc gccattcccc aggtgctgga
     1501 gttctacggc gccaccgaag gcaatgtgtc gatgttcaac ttcgacggcc atgtcggggc
     1561 gatcggccgg ttgccgaaat ggctgaagaa gcgggtcaac gcgcggctgg tgcagttcga
     1621 cgtcgagacc gagcagcccg ttcgcggcgc ggacggccgc tgcatcgagt gcggccccgg
     1681 ccagatcggc gagtgcatcg gcaagatcga caccagcgac ccgcggacca acttcaccgg
     1741 ctacgccgac aaggcggcga ccgagaagaa ggtgctgcac gacgtgttcg agaagggcga
     1801 cgcctggttc cgcacgggcg acctcatgcg ccaggatgcc gagggctatt tctatttcgt
     1861 cgaccgcatc ggcgacacct tccgctggaa gggcgagaac gtctccaccg gcgaggtcgc
     1921 cgaccgcctg gccgaggcgc cgggcgtgct cgaggccaat gtctacggcg tggccgtgcc
     1981 gggccaggac ggccgggccg gcatggtcgc gctgaccatc gacgacgact tcagcctgac
     2041 ggcgttctgg gatcacgtgg accgcgaact gccgtcctac gcccagccgc tgtttctgcg
     2101 gctgcagcgc cagatcgaaa ccacgggcac cttcaagtac cgcaaggtcg acctggtcga
     2161 ggagggctat gacccggcca gggtccgcga tccgctgtac ttccgcaatc cggtcaagaa
     2221 ggcctggacc aggatcagca aggccgcctt cgacaagatc aacgcgggcg gcttcaggct
     2281 gtagcggcgc gggccggcgg cggcatggcc gcctgatgca gcgcccggtc gtcgagcggc
     2341 agctggtccg gcgcgaggct cagcaggccg acgggatggc tgagcggtcc ggacaccgcc
     2401 cggccgctga tgaacggaat gcgacgggcc aggcaggtct cgagttccac ggcgttgcgc
     2461 acgttggaga tcgacggcca gatgcgcttc tggcggaaca ggtcgatgcg ctcggcgaag
     2521 cggcggatgg cggcgatccg cgccagctgg ctggcctccg gcaacaccag ggtcacgctg
     2581 tggacggcct tgatcgccag ccgctcgacg gcgaaatccg gatcgccggt ctgcaggtcg
     2641 acgaccttga aataggcgtc gaggaacgcc ttgacctggg acagggccga gaaggacggg
     2701 tcgcgcggcg tgtcatagac cgacaccgcc aggttgctgc gacgcgtttc cggcagggtg
     2761 ttcagccagt gccgatagtc ctcgcgctcc tggcggtgaa tgagcgaggt atagcagacg
     2821 ggcagataga acatgcccga gaaccgcgcc tggcgcgcca gcaggtcgac cgccatggcg
     2881 atgatcgccg cgtcatgctc gcgggcgatg tcgcgctgcg cgggatgcag gccgtcgacc
     2941 aggttgaagc cggattcgcg attggacacc gcgcagccga cgaacgcctc ccgtccggcg
     3001 aaatagacgc cctggaagca gggcgccgcc ggcgccgcat ccgccgtcgc ccggaccggc
     3061 gccgcgggaa ggtcgggatt gggtgagccc gggaccgcga tcggccggac gtcgtcgacc
     3121 gtgatccgtg tcaggcggcc catcagcggt ggcgccagcg actcgctccg gacggccttg
     3181 cgcacctcat gctggtcaag gctggcgaag cgggccacct cgcccggctc cccctcgaac
     3241 acgatcagct cgacctcgcc ggcgccgccc gagccgaaca gccgcagctg caggtcgtag
     3301 gccagatcct gcaggatctt ctgctccagc ttcggggtgc gcggatcggc cagcaggaag
     3361 gccgacaggg acatcacctc gaaccgcctc ggccacgacc agcgggcctc caggaaggcg
     3421 caggcatagc ggttgatcag gccttcgagg cccgggcggc tgtgcagatc ggggggcgga
     3481 agtatccgca ggcaggcgaa ggcggtcatg cctcctgggt agccgacaca gatttacgct
     3541 tgtttttgcg agcggccttc agcctgcggt ttagtgtcgc gccggggttc agcccgggac
     3601 gacaatgtag cccgcgcgcg ggaagtgcac atggaccagg ccgacggccg gatcctcgcg
     3661 ggccagcgtc aacccgcggg gcgtggcggc gaccagggtc ccgcgcacgg ggtcgcggcc
     3721 atagtcgtcg gccatcacaa ccaccgccgc gc
//
LOCUS       DAORNX010000303         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18418_length_3749_cov_7.861397, whole genome shotgun sequence.
ACCESSION   DAORNX010000303 DAORNX010000000
VERSION     DAORNX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18418_length_3749_cov_7.861397"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLO65_12330"
     CDS             <1..1454
                     /locus_tag="PLO65_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD29079.1"
                     /translation="PAQLVRTAGAGPAIFAPPPGAPMSFADIFEKVSPAVVSIDVTSR
                     VDARSLRLPNFPFGPNGKPDDQNGAGPEDESGGGGRPRQQSSGSGFLISADGYIVTNN
                     HVIEGADEINVTLKDGRELKATLVGRDENTDLAVIKVQGSGFTFVTFENAAKPRVGDW
                     VIAVGNPFGLGGTATAGIVSAYGRNLNDESSSFVDYVQIDAPINRGNSGGPTFDIYGR
                     VIGVNSAIFSPTGGSVGIGFAIPADVADSITKQLISGGKVVRGYIGAQIQNFTPEMSQ
                     ALGLGNLKAAVVKAVTAGGPAAKAGLMIDDIVVSVNGVKVESSTALTREVARGAAGQV
                     LRLEILRDGHRRFIDIRSGVRPSNDELASVLQPDAGADENSGKGAATPGATRVQVLGM
                     GLTPLDAAARKRYGIAADVQGVLIESLRGDSDAAERGITTGNVITSVNLKAVRTPADV
                     AAAVEAARKAGRPTILLSVLFRGQLASVPVKVD"
     gene            1609..2280
                     /locus_tag="PLO65_12335"
     CDS             1609..2280
                     /locus_tag="PLO65_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD29080.1"
                     /translation="MRILIIEDDIEAAEAMARGLGEAGYDCLHAADGAAGLAEAQKRG
                     FDVLIVDRMMPKMNGVQVVETLRREGDATPVLFLSALGEVGDRVDGLKAGADDYLVKP
                     YAFPELIARVEALSRRRETGAVATTLKVGDLEMNLIARTVHRGTTEIDLQPREFQLLE
                     FMMRHAGQSVTRTMLLEKVWEYHFDPQTNVIDVHISRLRSKIDKGFERQMLQTVRGAG
                     YRLDP"
     gene            2299..3705
                     /locus_tag="PLO65_12340"
     CDS             2299..3705
                     /locus_tag="PLO65_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD29081.1"
                     /translation="MRLPRIFRTTPFRLTLLFLAMFAAAAAAFLAYAYLATAGEVTRR
                     ADDEIRREMNSLEAIYRRGGVSSLNQALIERAVGERPFLYLLLDRQGRRITGSIEESP
                     IGETPEPGENWTTFQVTDTDSDGGEVKRPARGLQERLEGGEVLFVGADVGESEGFVTG
                     IVRALWGAGALVILLGLAGGVIVSRNVSRHMAGLNETVAAVRAGDLKARARVRGARDE
                     YDELAEGLNEMLDRLERSMGGLRHAGDAIAHDLRSPLTRLRARMEAALIEVEAGKGDP
                     KQALVQALDDADNVLTTFNAVLAIARLQAAGSAPDQTLFDPGQLAADMAELYEPLCEE
                     KGIDFRAEVTPQLTVKAARPILAQALANIIDNAIKYTPAGGAVMVRVRRRSSGEIEFS
                     ITDNGPGVPLEDRARVVQRFVRLENSRNEPGSGLGLSLVAAVAEAHGGRLELDEGPGE
                     YNGFGPGLRVALVLPRVE"
BASE COUNT          585 a         1278 c         1328 g          558 t
ORIGIN      
        1 agccggccca gctggtgcgg acggccggcg cggggccggc gatcttcgcg ccgccgcccg
       61 gcgcgccgat gtcgttcgcc gacatcttcg aaaaggtctc gccggccgtg gtgtcgatcg
      121 acgtgacctc gcgcgtcgac gcccggtccc tgcgactgcc gaacttcccg ttcggcccca
      181 acggaaagcc cgacgaccag aacggcgcgg gccctgaaga cgagagcggc gggggcgggc
      241 ggccccgcca acagtcttcc gggtccggct tcctgatctc ggccgacggc tacatcgtca
      301 ccaacaacca cgtcatcgag ggcgccgacg agatcaacgt cacgctcaag gacgggcgcg
      361 agctcaaggc caccctggtc ggccgcgacg agaacaccga tctggccgtg atcaaggtgc
      421 agggctcggg cttcaccttc gtcaccttcg agaacgcggc caagccgcgc gtcggcgact
      481 gggtcatcgc cgttggcaac cccttcggtc tcggcggcac cgccaccgcc ggcatcgtgt
      541 cggcctacgg ccgcaacctc aacgacgagt cctcgagttt tgtcgattac gtgcagatcg
      601 acgcgccgat caaccgcggc aactcgggcg gcccgacgtt cgacatctat ggccgggtga
      661 tcggcgtcaa cagcgccatt ttctcgccca ccggcggctc ggtcggcatc ggcttcgcca
      721 ttccggccga cgtggccgac agcatcacca agcagctgat cagtggcggc aaggtggtgc
      781 gcggctacat cggggcgcag atccagaact tcacgccgga aatgtcgcag gcgctgggcc
      841 ttggcaatct gaaggcggcg gtggtcaagg ctgtcaccgc cggcggccca gcggccaagg
      901 ccgggctgat gatcgacgac atcgtcgtgt cggtgaacgg ggtgaaggtg gagtcctcca
      961 ccgccctgac ccgcgaagtc gcccgcggcg cggccggcca ggtgctgcgc ctggagatcc
     1021 tgcgcgacgg tcacaggcgg ttcatcgaca tccgctcggg cgtgcggccg tccaacgatg
     1081 agctggccag cgtccttcag ccggacgccg gcgccgacga gaacagcggc aagggcgccg
     1141 cgacgcccgg cgcgacccgg gtgcaggtgc tgggcatggg cctgacgccg ctcgatgcgg
     1201 cggcgcgcaa gcggtatggc atcgccgccg atgtccaggg cgtgctgatc gagagcctgc
     1261 gcggcgactc cgacgccgcc gaacggggca tcaccacggg caatgtgatc acctcggtga
     1321 atctcaaggc cgtgcgcacc cccgcggatg tggccgcggc ggtcgaggcc gccaggaagg
     1381 ccggccggcc gacaatcctg ctgtcggtgc tgttccgcgg ccagctggcc tcggtgccgg
     1441 tcaaggtcga ctaggccccc ttcgccgatc cccggatccg tcgccaggcc ggcggcggtc
     1501 cgggtggtcg gcggcggatt acactatcgt atgttgagcg tgccgccggg agcggggtag
     1561 tttgccggcg tcggcggatt ctctccgcag gccacatcgg ggcgaaccat gcgaatcctg
     1621 atcatcgagg atgatatcga ggcagcggag gccatggcgc gcggcctggg cgaggccggt
     1681 tacgattgcc tgcacgccgc cgatggcgcc gccggcctgg ccgaagccca gaagcgtggc
     1741 ttcgacgtgc tgatcgtcga tcgcatgatg cccaagatga acggcgtgca ggtcgtcgaa
     1801 accctgcgtc gcgaaggcga tgcgacgccg gtgctgttcc tgtccgccct tggcgaggtc
     1861 ggcgaccggg ttgacggcct gaaggccggc gccgacgact acctggtcaa gccctacgcc
     1921 tttcccgaac tgatcgcccg tgtcgaggcc ctgtcgcgcc gacgcgaaac cggcgccgtg
     1981 gccaccacgc tcaaggtcgg cgaccttgaa atgaacctga tcgcccgcac cgtgcaccgc
     2041 ggaacgaccg agatcgacct gcagccgcgc gaattccagc tgctggagtt catgatgcgc
     2101 cacgccggcc agtcggtgac ccgcaccatg ctgctggaga aggtctggga ataccatttc
     2161 gatccccaga ccaatgtcat cgacgtgcac atctcccgcc tgcgctcgaa gatcgacaag
     2221 ggtttcgaac ggcagatgct gcagaccgtg cgtggcgccg gctaccggct cgacccttag
     2281 cgggccggcg ccgcagccat gcgcctgccc cggatatttc gcaccacgcc gttccggctg
     2341 acgctgctgt tcctggcgat gttcgccgcg gcggccgccg ccttcctggc ctatgcctat
     2401 ctggcgacgg ccggggaggt gacccgtcgg gccgacgacg agatccgccg cgagatgaat
     2461 tcgctggagg cgatctatcg ccgcggcggg gtctcctcgc tcaaccaggc cctgatcgag
     2521 cgggccgtgg gggaacggcc tttcctctat ctgctgctcg accggcaggg caggcggatc
     2581 accggctcga tcgaggagtc gccgatcggt gaaacgccgg aaccgggaga gaactggacc
     2641 accttccagg tcaccgacac cgactccgac ggcggcgagg tcaagcggcc ggcccgcggc
     2701 ctgcaggaac ggctggaggg cggcgaggtg ctgttcgtcg gcgccgacgt cggcgaatcc
     2761 gagggattcg tcaccggcat tgtccgcgcg ctgtggggcg ccggggccct ggtcatcctt
     2821 cttggtctgg ccgggggggt gatcgtcagc cgcaacgtca gccgccatat ggccggactg
     2881 aatgaaaccg tggccgccgt ccgggccggc gacctgaagg cgcgggcgcg ggtgcggggc
     2941 gcccgggatg aatacgatga gctggccgag gggctcaacg agatgctcga ccgactcgaa
     3001 cgctcgatgg gcgggctgcg acacgccggc gacgccatcg cccatgacct gcgttcgccg
     3061 ctgacccggc tgcgcgcgcg gatggaggcg gcgctgatcg aggtcgaggc gggcaagggc
     3121 gacccgaaac aggcgctggt ccaggccctc gatgacgccg acaacgtctt gacgaccttc
     3181 aatgcggtgc tggccatcgc ccggttgcag gcggccggca gcgcgccgga ccagaccctg
     3241 ttcgatcccg gtcagctggc cgctgacatg gccgagcttt acgagccgct gtgcgaggaa
     3301 aagggcatcg acttccgggc cgaggtgacg ccgcagctga ccgtcaaggc cgcccggccg
     3361 atcctcgccc aggcgctggc caacatcatc gacaacgcca tcaagtacac ccccgccggc
     3421 ggagcggtga tggtccgcgt ccggcggcgc tcgtccggcg agatcgaatt ctcaattacc
     3481 gacaatggac ccggcgtgcc gctggaagac cgcgccaggg tggtgcagcg gtttgtccgg
     3541 ctggagaata gccgcaacga gccggggtcg ggactcgggt tgtcgctggt ggccgcggtg
     3601 gccgaggccc acggcggccg gctggagctc gacgagggcc ccggcgagta caacggcttc
     3661 ggtccgggcc tgagggtggc cctggtgctg ccgcgtgtgg agtaggggcg ccatggtccc
     3721 gaccagccgc cgccgcctgc tggccggcg
//
LOCUS       DAORNX010000304         3736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18518_length_3736_cov_7.193154, whole genome shotgun sequence.
ACCESSION   DAORNX010000304 DAORNX010000000
VERSION     DAORNX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18518_length_3736_cov_7.193154"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO65_12345"
     CDS             complement(<1..216)
                     /locus_tag="PLO65_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HRD29082.1"
                     /translation="MALAGFAVLGAGWRLTAGPTAGVVQPLDPAALTALQHIAFARAE
                     AQPGFDHGQNISVEVRPGETLEAAVLRS"
     gene            complement(420..833)
                     /gene="arsC"
                     /locus_tag="PLO65_12350"
     CDS             complement(420..833)
                     /gene="arsC"
                     /locus_tag="PLO65_12350"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062110.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRD29083.1"
                     /translation="MTSAAVTIFHNPACGTSRNTLAAIRQSGVEPTVVEYLKAGWTRP
                     QLEDLLAAMGKTPRDILRARGTPAEELGLTDPAATDGTILDAMVAHPILVERPIVRTP
                     RGAALCRPSEKVFDLLDSAPAEFVKEDGERVTPGR"
     gene            complement(830..1873)
                     /gene="arsB"
                     /locus_tag="PLO65_12355"
     CDS             complement(830..1873)
                     /gene="arsB"
                     /locus_tag="PLO65_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRD29084.1"
                     /translation="MSIFERYLTLWVALCIVAGVALGQLFPGAAQAIGGLEIARVNLA
                     VAGLIWLMIIPMLMKVDFGAMRQVGRHWRGISVTLFINWAVKPFSMALLAWLFIGTLF
                     RPLLPAAQIDSYIAGLIILAAAPCTAMVFVWSNLTKGEPNFTLSQVALNDTIMVLAFA
                     PIVALLLGLSAITVPWETLLLSVGLYIVVPVIIAQVVRRMLLARGAPALERALGRLGP
                     VSILALLATLVLLFAFQGEQIIAQPMVIALLAVPILIQVYFNAGLAYLLNRASGEAHC
                     VAGPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLVEVPVMLTVVAIVNRSRD
                     WYERGLKSPGATP"
     gene            complement(1876..2370)
                     /locus_tag="PLO65_12360"
     CDS             complement(1876..2370)
                     /locus_tag="PLO65_12360"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRD29085.1"
                     /translation="MTRTVLFLCTGNSARSILAECILNRLGGGRFRAYSAGSMPTGTV
                     NPHALRLLEQMNHPTDGLRSKSWEEFDRAHNPDAPELNFVFTVCDNAAGEVCPVWPGQ
                     PMTAHWGVPDPAAVTGSDAEIGLAFADVYGRLHNRLELLVNLPMASLDRMTLQARLDG
                     IGKA"
     gene            complement(2370..2720)
                     /locus_tag="PLO65_12365"
     CDS             complement(2370..2720)
                     /locus_tag="PLO65_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD29086.1"
                     /translation="MEQTDAVAALSALAHPGRLAVFRLLVKAGAEGLPAGRIAEATGS
                     LSNTLSANLNILTQAGLARSRRDGRQIIYTADYDVMRDLLTFLVEDCCQGEPAICGPL
                     ASIASGASCGAKTC"
     gene            complement(2868..3305)
                     /locus_tag="PLO65_12370"
     CDS             complement(2868..3305)
                     /locus_tag="PLO65_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HRD29087.1"
                     /translation="MKRLHLHIAVEDLTRSIGFYSTLFGSEPSVVKDDYAKWMLEDPR
                     VNLAISQRDRVAPGVDHVGVQAESAAELAEMATRLKAAGETTFDQEATTCCYARSDKS
                     WVTDPSGVRWETFHTLGEATTYGEDEVMPAATAAGVAKASCCG"
     gene            complement(3387..>3736)
                     /locus_tag="PLO65_12375"
     CDS             complement(3387..>3736)
                     /locus_tag="PLO65_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29088.1"
                     /translation="VPRTFNGGPQFRYPAARGLALDLKADGGLRALAIDGKAVPFQTR
                     PREWTRIRLTGAAGGELVITPANPGPLDVRYAVSEQGWPAGARPLPPRPADVMAFDRS
                     DSSVVIGTLSAAF"
BASE COUNT          607 a         1232 c         1343 g          554 t
ORIGIN      
        1 gctgcgcagc acggccgcct cgagtgtttc gccggggcgg acctcgacac tgatgttctg
       61 gccgtggtcg aagcccggct gggcctcggc gcgggcgaag gcgatgtgtt gcagggcggt
      121 gagggcggcg ggatcgagcg gctggacgac gcccgcggtc gggccggcgg tcagcctcca
      181 gccggcgccg agcaccgcga aaccggccag cgccacaaga accttcggcg cgaaacggac
      241 tgtcgggcgc ctgggatcga attcctgcat cgttccccca tcagtttacg atgccgtacg
      301 agcgtcagaa tggcacggct gggatgcccc ccagcaatcg gcgggccggt catgcccggc
      361 cacgcccccg aaacgcaagt aatattcggt gcttcgtcct atttgtcacg ccccggcgtt
      421 cagcgaccgg gggtcacgcg ctcgccgtct tccttgacga attcggcggg ggccgagtcc
      481 agcagatcga acaccttttc cgatggccgg cacagcgccg cgccgcgcgg cgtgcgcacg
      541 atcggccgct cgacgaggat cggatgggcg accatggcgt cgagaatggt cccatcggtg
      601 gccgccggat cggtcaaccc cagttcctcc gccggcgtgc cccgcgcgcg caggatgtcg
      661 cgcggcgtct tgcccatggc cgccagcagg tcctcgagtt gcggccgggt ccagccggcc
      721 ttgaggtatt cgacgacggt cggctcgacg cccgactgcc ggatcgcggc cagggtgttg
      781 cgcgaggtgc cgcaggcggg gttgtgaaag atggtgacag cggcgctggt catggcgtgg
      841 ctccgggaga cttcaggccg cgttcgtacc agtcccgtga gcgattcacg atggccacga
      901 cggtcagcat gaccggcacc tcgaccagca ccccgaccac cgtggccagg gcggcgccgg
      961 actggaagcc gaacaggctg atggccgcgg cgacggccag ctcgaagaag ttgctcgccc
     1021 cgatcagggc cgagggtccg gccacgcagt gggcctcgcc actcgcgcgg ttcagcaggt
     1081 aggcgagacc ggcgttaaaa tagacctgga tcaggatcgg aacggccagc agcgcgatga
     1141 ccatcggctg ggcgatgatc tgctcgccct ggaaggcgaa cagcagcacc agcgtggcca
     1201 gcagcgcgag gatcgacacc ggtccgaggc ggcccagcgc gcgctccagc gccggcgccc
     1261 cgcgggccag cagcatgcgg cggacgacct gggcgatgat caccggcacg acgatgtaca
     1321 ggccgaccga cagcagcagc gtctcccacg gcacggtgat ggccgacagg ccgagcagca
     1381 gcgccacgat cggggcgaag gccaggacca tgatggtgtc gttgagcgcg acctggctta
     1441 gcgtgaagtt cggctcgccc ttcgtcaggt tgctccagac gaacaccatg gccgtgcacg
     1501 gcgcggccgc caggatgatc aggccggcga tgtagctgtc gatctgcgcc gccggcagca
     1561 gcggccggaa cagggttccg atgaacagcc aggccagcag ggccatggag aagggcttga
     1621 ccgcccagtt gatgaacagc gtcacgctga tgccgcgcca gtggcggccg acctggcgca
     1681 tggcgccgaa gtcgaccttc atcagcatcg ggatgatcat cagccagatc aggccggcga
     1741 ccgccaggtt gacgcgcgcg atctccagcc cgccgatggc ctgggccgcg ccgggaaaca
     1801 gctggccgag cgctacgccg gcgacgatgc acagtgcgac ccacagggtc agatagcgtt
     1861 cgaagatgga catggtcagg ccttgccgat gccgtcgagg cgggcctgca gggtcatccg
     1921 gtcgaggctg gccatcggca ggttgacgag cagttcgagg cggttgtgca gccggccata
     1981 gacgtctgcg aaagccaggc cgatctcggc gtcgcttccg gtcacggcgg ccggatcggg
     2041 cacgccccag tgggccgtca tcggctggcc gggccagacg gggcagacct cgccggcggc
     2101 gttgtcgcag acggtgaaga caaaattcag ctcgggcgcg tcgggattgt gggcgcgatc
     2161 gaactcctcc cacgacttcg agcgcaggcc gtcggtcggg tggttcatct gttcgagcag
     2221 gcgcagcgca tgcggattga cggtcccggt cggcatcgag ccggccgaat aggccctgaa
     2281 ccgcccgccg ccgagccggt tgaggatgca ttcggcgagg atcgagcgcg ccgagttgcc
     2341 ggtgcagaga aacaggacgg tccgggtcat cagcaggtct tcgcgccaca gctcgcgccg
     2401 ctggcgatgc tcgccagcgg gccacagatg gccggctcgc cctggcagca gtcctcgacc
     2461 aggaaggtca gcaggtcgcg catgacgtcg tagtcggcgg tgtagatgat ctgtcggccg
     2521 tcgcgccggg agcgggccag gcccgcctgg gtcagaatgt tgaggttggc cgacagggtg
     2581 ttgctgaggc tgcccgtggc ctcggcgatg cggccggccg gcaggccttc ggccccggcc
     2641 ttgaccagca ggcggaagac ggccagccgg ccgggatggg cgagggccga cagggcggcg
     2701 acggcgtcag tctgttccat gattcgagaa tagccgaatt ttgtgacagc tgtgcgtcct
     2761 tcgccacggc cgctacgcgg cctgctcagg atgacgtgcg ggggtggcgc gtcacacctc
     2821 taagttatgg tgagcagcgc ccgaaaggcg cgtgtcgaac cacgcactca gccgcaacaa
     2881 ctcgccttcg ccacgcccgc ggccgtcgcc gccggcatca cctcgtcctc gccgtaggtc
     2941 gtcgcctcgc cgagggtgtg gaaggtctcc cagcgcacgc ccgacgggtc ggtgacccag
     3001 ctcttgtccg acctggcgta gcagcaggtg gtggcttcct ggtcgaaggt cgtctcgccg
     3061 gcggccttca gccgcgtggc catctcggcc agctcggcgg cgctctcggc ctgcacgccg
     3121 acatggtcga caccgggcgc gacgcggtcg cgctgcgaga tggccaggtt cacgcgcgga
     3181 tcctcgagca tccacttggc gtagtcgtcc ttcaccaccg agggctcgga gccgaacagg
     3241 gtggagtaga agccgatcga gcgggtcagg tcctcgacgg cgatgtgcag gtgcaggcgc
     3301 ttcatggcgg tctcctataa atctattatt cgagaaatat agaaatatag ggccgagtca
     3361 agaggtgggc ggagacgcct ccccggtcag aacgcggcgg agagggttcc gatcacgacg
     3421 ctggagtctg accgatcgaa cgccatgacg tcggccggcc tcggcggcaa cggcctcgcc
     3481 ccggcgggcc agccttgctc gctgaccgcg tagcggacgt ccagcggccc cgggttggcc
     3541 ggggtgatca ccaactcgcc gcccgccgcc ccggtcagcc ggatccgcgt ccattcccgc
     3601 ggccgggtct ggaacgggac ggccttgccg tcgatcgcga gggcgcggag cccgccgtcg
     3661 gccttcaggt ccagagccag cccgcgggcg gcgggatagc ggaactgcgg tccaccgttg
     3721 aaagttcttg gcaccc
//
LOCUS       DAORNX010000305         3732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18551_length_3732_cov_5.617623, whole genome shotgun sequence.
ACCESSION   DAORNX010000305 DAORNX010000000
VERSION     DAORNX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18551_length_3732_cov_5.617623"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLO65_12380"
     CDS             complement(<1..307)
                     /locus_tag="PLO65_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRD29089.1"
                     /translation="MIDMAWIDAALTGARPQAVGALLRYFRDLDTAEEAFQDACLRAL
                     KSWPVNGPPRDPAAWLIMVGRNAGIDGARRRAKQTVLPDETVLSDIDDAETPLAERLD
                     "
     gene            complement(304..669)
                     /locus_tag="PLO65_12385"
     CDS             complement(304..669)
                     /locus_tag="PLO65_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD29090.1"
                     /translation="MLYAILCYNAEDVVMSWSQEEDDAVMAGLDVVHQRLNAENKLGP
                     SLRLMPTTAATTLHKSQDLVVDGPYAETKEQLLGLYIVDISGLDEGLEIARELGKANP
                     GGAYELRPLRLYLPGASLA"
     gene            803..1345
                     /locus_tag="PLO65_12390"
     CDS             803..1345
                     /locus_tag="PLO65_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD29091.1"
                     /translation="MDRDLDQVLDEYLVLLARDGSRPALAQLVGRWSPRVARHVARTL
                     GDREAAADVTQETWLAALRGLERLDDPARFPAWLYAIATRKCVDLIRSRARMRRLGAG
                     LKAQVAPSQGADSDDGLDLTAALARLPADQRLVASLFYGEDLDLETIAAVAAVPVGTV
                     KSRLHHARAALRTILEGGQT"
     gene            1342..1722
                     /locus_tag="PLO65_12395"
     CDS             1342..1722
                     /locus_tag="PLO65_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29092.1"
                     /translation="MTQIDDAIRQALSAEDARALEAFSGEQPMLFQALATLGAGPNRL
                     INIGGWIGGFALFAVGLFFATRFYAAADPREMALWGAATVMAFVGLGLIKIWFWMEIH
                     KNQIIREVKRLELQVARLAARGQV"
     gene            complement(1761..2792)
                     /locus_tag="PLO65_12400"
     CDS             complement(1761..2792)
                     /locus_tag="PLO65_12400"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRD29093.1"
                     /translation="MTTNPSEPRPWRKILAAYQRPSHARSILEIVLSAAPLAAIWAAA
                     WVAAVFGLWWLALLLSIPAAAFLVRLFMVQHDCSHQSFFRSPAANDWTGRLIGVLTMT
                     PYHCWRRSHAIHHATSGDLDRRGLGDIATLTVAEYRALSLLRRIGYRLYRNPIVLFVI
                     GPAWVFVIEQRMPMGLMKEGWKPWLSAMGTNLAIAAIITAVILLGGWKGLLLIHLPTI
                     LLAASIGVWLFYVQHQFENAYWARKGEWDHTTAALHGSSHYDLPQPLRWMTANIGVHH
                     VHHVASRIPYYRLTRVLKDHPELRNVSRLGLIESFKTVSLALWDEGGRRLISFRQLRQ
                     LRRAERAAA"
     gene            complement(3013..3435)
                     /locus_tag="PLO65_12405"
     CDS             complement(3013..3435)
                     /locus_tag="PLO65_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29094.1"
                     /translation="MTQPLRRLIDLPGVADLETRALMKPSLAEPDARADLPEIDAVCR
                     ANFGLTPDEADSVALPADWDGIDRKPPAQQVFAFEDAGWDVTDKRRPLRMLGHYALPL
                     WLAIRGVAGQLPYEAPPAEASDRWASGLEADARAYRKR"
     gene            3631..>3732
                     /locus_tag="PLO65_12410"
     CDS             3631..>3732
                     /locus_tag="PLO65_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HRD29095.1"
                     /translation="MAGLKDKRGFIDKDRLDLSERQAVEYWMRRWGVT"
BASE COUNT          548 a         1265 c         1354 g          565 t
ORIGIN      
        1 cgtcgagccg ctcggccagc ggcgtctcgg cgtcgtcgat gtcggacagc accgtctcgt
       61 cgggcaggac cgtctgtttc gcgcgccgcc gggccccgtc gatgccggcg ttgcggccga
      121 ccatgatcag ccaggccgcc gggtcgcgcg gcgggccatt gaccggccag ctcttcagcg
      181 cccgcaggca ggcgtcctgg aatgcctcct cggcggtgtc gagatcgcgg aaatagcgca
      241 gcagcgcccc cacggcctgg ggacgggcgc cggtcagggc cgcatcgatc caggccatgt
      301 cgatcatgcc agcgacgcgc ccggcagata gaggcgcagc ggtcgcagtt cataggcccc
      361 gcccggattg gccttgccca gttcgcgggc gatctcgaga ccctcgtcga ggccggagat
      421 gtcgacgata tagagcccca gcagctgttc cttggtctcg gcgtagggcc cgtcgacgac
      481 caggtcctgg ctcttgtgca gggtggtggc ggccgtcgtc ggcatcagcc gcagcgatgg
      541 ccccagcttg ttctcggcgt tcagccgctg gtggaccacg tcgagtccgg ccatgaccgc
      601 gtcgtcctcc tcctgcgacc aggacatgac gacgtcttcg gcgttgtaac aaaggatggc
      661 gtacagcacg ggcggatcct ctctccgtca caggacgaac cagtatgccg cgcgccgaca
      721 cgggcgccga gaaaatcgca tccctgcgtt gaacccgttc cggcggcggg gcgactattc
      781 cccggaaggc cggaggctgg gcttggaccg cgatctcgat caggtgctcg acgaatatct
      841 cgttctgctc gcccgcgacg gctcgcggcc ggcgctggcg cagctggtcg gtcgctggtc
      901 gccgcgggtt gcgcgccacg tcgcccgcac cctcggcgac cgggaggcgg cggcggacgt
      961 gacgcaggag acctggctgg cggcgctccg gggtctggag cgactcgacg accccgcgcg
     1021 gttccctgcc tggctctacg ccatcgccac ccgcaagtgc gtcgatctga tccggtcgcg
     1081 ggcccgcatg cggcggctgg gggcgggatt gaaggcgcag gtcgccccgt cccagggcgc
     1141 tgattccgac gacggcctcg atctcacggc cgccctggcg cgcctgcccg ccgaccagcg
     1201 gctggtggcc agcctgttct acggcgagga cctcgacctg gagaccatcg ccgccgtcgc
     1261 cgccgttccg gtcggcaccg tcaaatcccg cctgcaccac gcgcgggcgg ccctcaggac
     1321 cattctggaa ggaggtcaga catgacccag atcgacgacg ccattcgcca ggccctgtcc
     1381 gccgaggacg cccgggcgct ggaggcgttt tccggcgagc agccgatgct gttccaggcg
     1441 ctggcaaccc tcggcgccgg ccccaaccgg ctgatcaaca tcggcggctg gatcggcggc
     1501 ttcgccctgt tcgcggtcgg gctgttcttc gccacgcgct tctacgccgc ggccgacccg
     1561 cgcgagatgg ccctgtgggg cgccgccacg gtcatggcct tcgtcgggct ggggctgatc
     1621 aagatctggt tctggatgga gatccacaag aaccagatca ttcgcgaggt gaagcggctg
     1681 gaactgcagg tcgcccgcct ggccgcccgc gggcaggtct gacagccggg atgctgtgcc
     1741 tgacgccggg cgggcggctt ctaggccgcc gcccgttcgg cccgccgcag ctggcgcagc
     1801 tggcggaacg agatcagccg gcggccaccc tcgtcccaca gagccagcga gacggtcttg
     1861 aagctctcga tcaggcccag ccggctgacg ttccgcaact cgggatggtc cttcagcacc
     1921 cgcgtcaggc ggtagtaggg aatgcggctg gcgacgtgat gcacatggtg caccccgatg
     1981 ttggcggtca tccagcgcag cggctgcggc aggtcatagt gcgagctgcc gtgcagcgcg
     2041 gccgtggtgt ggtcccactc gcccttgcgc gcccagtagg cgttctcgaa ctggtgctgc
     2101 acgtagaaca gccagacccc gatcgaggcc gccagcagga tggtcggcag gtggatcagc
     2161 agcagaccct tccagccgcc cagcaggatg acggcggtga tgatggcggc gatggccaga
     2221 ttggtgccca tggcgctgag ccagggcttc cagccctcct tcatcaggcc catcggcatg
     2281 cgctgctcga tcacgaacac ccaggccggg ccgatcacga acagcacgat cgggttgcga
     2341 tacaggcgat agccaatccg cctgagcaat gacagggcgc ggtactcggc caccgtcagg
     2401 gtggcgatgt cgcccaggcc ccggcggtcg aggtcgccgg aggtggcgtg gtggatggcg
     2461 tgcgaccggc gccagcagtg atagggcgtc atggtcagca ccccgatcag ccggccggtc
     2521 cagtcgttgg cggccgggct gcggaagaac gactggtggc tgcagtcgtg ttgcaccatg
     2581 aacagccgca ccaggaaggc ggcggccgga atggacaaca gcagggccag ccaccagagg
     2641 ccgaagacgg ccgccaccca tgcggcggcc cagatcgccg ccagcggggc ggcgctcagg
     2701 acgatctcga gaatcgagcg ggcatgactg gggcgctggt aggcggcgag aatcttgcgc
     2761 caggggcgcg gttcggaggg gttcgtggtc aaggtctctt cccggggccg cggcgacggt
     2821 ttcttggcga acggtctagc gcgactgtac gacggagatg tttaaggccc cgctgcgagt
     2881 cgcgcgagac gccgcggcgt cgcggctgcg cacgaagcgg agcgggcgtg gcctgacggg
     2941 tacgccgcga cttgggctta ttggcggcaa atggcagcgg ggacacgtgc ctgacggtgc
     3001 atgtccccgc ggctaacgtt tccggtaggc cctggcgtcg gcctccaggc cgctggccca
     3061 gcggtcgctc gcctcggccg gcggggcctc gtacggcagc tggccggcca cgccgcggat
     3121 cgccagccac aggggcaggg cgtagtggcc cagcatgcgc agcgggcggc gcttgtcggt
     3181 cacgtcccag ccggcgtcct cgaaggcgaa cacctgctgg gccggcggct tgcgatcgat
     3241 gccgtcccag tcggccggca gcgccacgga atcggcctcg tcgggcgtca ggccgaagtt
     3301 cgcgcggcag acggcgtcga tctcgggcag gtcggcgcgc gcatcgggct cggcgaggga
     3361 cggcttcatc agcgcccggg tctccaggtc ggcgaccccg ggcaggtcga tcaggcggcg
     3421 aaggggttgg gtcatggagg cggtttagcc ctcccgcgcc gtcgcgccaa cgtgcgtccg
     3481 tcgacacggc cgcgtcgcgg cctgctcagg atgacgtgtc gttgcccaca tcgtggaggg
     3541 caacactcga cgggcgggtg tcgaaccgcg caccctgtcg ccgggcgcga tttggcgcta
     3601 tcgaaggcca tcaatcccgg cggagcgccc atggccggcc tgaaggacaa gcgtggcttc
     3661 atcgacaagg accggctgga cctctccgag cgccaggccg tcgagtactg gatgaggcgc
     3721 tggggtgtga cc
//
LOCUS       DAORNX010000306         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18579_length_3728_cov_8.253199, whole genome shotgun sequence.
ACCESSION   DAORNX010000306 DAORNX010000000
VERSION     DAORNX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18579_length_3728_cov_8.253199"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1174)
                     /gene="recA"
                     /locus_tag="PLO65_12415"
     CDS             complement(107..1174)
                     /gene="recA"
                     /locus_tag="PLO65_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRD29096.1"
                     /translation="MNQAALRLVTRDEGDKQRALEAALAQIDRAFGKGSVMRLGSKSK
                     IEVEAVPTGSLGLDIALGIGGLPKGRVVEIYGPESSGKTTLALHVVAEVQKAGGTAAF
                     VDAEHALDPQYARKLGVNLDDLLVSQPDTGEQALEITDTLVRSNAVDVIVVDSVAALT
                     PRAEIEGEMGDSLPGLQARLMSQALRKLTASISKAGTLVIFINQIRMKIGVMYGSPET
                     TTGGNALKFYASVRLDIRRTGSVKVRDEIVGNNVRVKVVKNKVAPPFREVEFDIMYGE
                     GISKLGEIIDLGVKAGVIDKSGSWFSFGDQRIGQGRDNVREFLKKNPDIAQQIEDAVR
                     GKSEIIEEELLVGPSEDGDEG"
     gene            1660..3579
                     /locus_tag="PLO65_12420"
     CDS             1660..3579
                     /locus_tag="PLO65_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29097.1"
                     /translation="MTIGLFTALPTTSVPTGDDVIRTTGYMTVGKGAGFYKRVATEPT
                     ALPASAYSADADDNWWALVEEAPNAYQFGAAGDGSTDDAAAINDGLLYAANATYGGGV
                     LYLLAAEYKVCAQILHPNFVCLYGWPGAKILRNWTSTNGNKYASATIRATDAIDYAAY
                     RVTAPPYYLVVEDGQYADSCRLGGSLYIGMTVCAREEADGDPSMRMCCAYYNGCDRLV
                     IDDGVTLERGGRDWCLVLHGNDIRVGSVNIIGGANVYEDGIHVMGGRNGSIIGAHVQA
                     GDDALAFGSNYNQPISGWIAMGCQLNSNAAHCIRFIQDRTGFDEPPLIDQEIESILIS
                     GCTGQGGVIANGVIRVDDPAMMISARILDMDLTHSTAEVHTGDNRDAIRLIGGNVTIT
                     GILRSPVRSALNASAGTTGTVDIVSDDAGQVSGYPAVLLTSVGEMVVDGRYQAYDFQG
                     VKILNCSDVIRSRFLDVYNIGNGCSGVDVVGSSAGYDAGHLKVTAEAGATSARGVGGV
                     GGSVLRIGGFSPRGVRPFGNPTTPASIAAAFLPGEFRSAYVAFGATTTYGDGVLKLLG
                     EGDATDDLLNITNGYVGQRLTVMNGMADPTTTTITIKSTGNIAVASNRVLNAGWSQVE
                     LLWNGSSWIETAFWV"
BASE COUNT          660 a         1185 c         1197 g          686 t
ORIGIN      
        1 ggagccggac gtccttcgat cgcggcggac cccgcggggc cacgcgggcg gcgggtgggc
       61 agacgcccgg cgcggagagg cgcgccgcga accgctcggg gccggatcag ccctcgtcgc
      121 cgtcctcgga ggggccgaca agcagctcct cctcgatgat ctcggacttg ccacgcacag
      181 cgtcctcgat ctgctgggcg atgtcggggt tcttcttgag gaactcgcgg acgttgtcgc
      241 ggccctggcc gatgcgctgg tcaccgaacg agaaccagga gcccgacttg tcgatcaccc
      301 cggccttgac gcccaggtcg atgatctcgc ccagcttgga gatgccctcg ccgtacatga
      361 tgtcgaactc gacctcgcgg aacggcgggg cgaccttgtt cttgaccacc ttgacccgga
      421 cattgttgcc gacgatctcg tcgcggacct tgaccgaacc cgtgcggcgg atgtcgagcc
      481 gcaccgaggc gtagaatttc agcgcgttgc cgcccgtggt cgtctccggg ctgccgtaca
      541 tcacgccgat cttcatgcgg atctggttga tgaagatgac cagggtgccg gccttggaga
      601 tggaggcggt cagcttgcgc agcgcctggc tcatcagccg cgcctgcagg cccggcaggc
      661 tgtcgcccat ctcgccctcg atctcggcgc gcggcgtgag ggccgccacc gagtcgacga
      721 cgatcacgtc gacggcgttc gagcgcacga gggtgtcggt gatctccagc gcctgctcgc
      781 cggtgtccgg ctgcgacacc agcaggtcgt cgagattgac ccccagcttg cgggcgtact
      841 gcgggtcgag cgcatgttcg gcgtcgacga aggcggccgt gccgccggcc ttctgcacct
      901 cggccaccac atgcagggcc agggtcgtct tgcccgagct ttccggcccg tagatctcga
      961 ccacccgccc cttgggcagg ccgccgatgc ccagggcgat atccagcccg agcgagccgg
     1021 tcggcacggc ctcgacctcg atcttgctct tggaacccag ccgcatgacc gagcccttgc
     1081 cgaaagcccg gtcgatctgc gccagcgccg cctcgagcgc gcgctgcttg tcaccttcgt
     1141 cgcgtgtcac gagtctcaaa gccgcctggt tcatgggagc cgtccttatt tcacgattct
     1201 gccggcccgc gtcgggcgac ggacgaacta tgatcacggt ttgttccggt tggcaatatg
     1261 ttctcggtcc tgcgcgtata aattgaatct tgtgtgaata cgccggtctc tgcgacgccg
     1321 ggcggatttc catttcgtta tgtcatccaa acgctcacgg ggcgctggtc ttgggacccg
     1381 ggacatggat gccggagaac cgggctcggc gcgcaattcg caaggctcgc gaaggagtgc
     1441 ttccggcagg gtaattcaag cacagattgt gttctcgtga tggggaccgt aggctgtcga
     1501 tttgcgggcg ccaggcgaca ggccaacttg tggtgctccc ccaggatcgg ttggagcgta
     1561 gagccgtggg cgggtcgatc atcaatccac cgatgcagcc aactggcctt ggacgatttc
     1621 gcgttccctg ttttccaatc tcaccttggc ggaagcgaca tgaccattgg gctgtttacg
     1681 gcactcccca cgacctcggt gccaaccggc gacgacgtga tccggacgac gggatacatg
     1741 actgtcggga aaggggcggg cttctacaag agggtggcga ccgagccgac cgccctgccg
     1801 gcctctgcct attcagccga cgccgacgac aactggtggg ccctggttga agaggcgccg
     1861 aacgcctacc agttcggcgc cgctggagac ggctcgaccg acgacgccgc ggcgatcaac
     1921 gacggcctgc tctatgcagc gaacgcaacc tacggcggag gcgtgctgta tctgctggcg
     1981 gctgaataca aggtatgcgc gcaaattctg catccgaact tcgtctgcct ctacggctgg
     2041 cccggcgcga aaatcctgcg caactggaca tcaaccaacg gcaacaagta cgcgagcgcg
     2101 acgatcaggg cgacggacgc gatcgactat gccgcctatc gggttaccgc gcctccctac
     2161 taccttgtgg tggaagacgg acagtacgcg gatagctgcc gcctgggcgg ttccctctat
     2221 atcggcatga ctgtctgcgc gcgcgaggag gccgacggcg atccgtcaat gcgcatgtgc
     2281 tgcgcatact acaacggttg cgaccggctc gtgattgatg acggcgtgac actggagcgg
     2341 ggtggccggg actggtgcct cgtgctccac ggcaatgaca tccgtgtcgg ctcggtgaac
     2401 atcatcggcg gcgccaacgt ctatgaagac ggaatccacg tcatgggcgg ccgcaatgga
     2461 tcgatcatcg gcgcgcatgt gcaggctgga gacgacgcgc tggctttcgg atccaactat
     2521 aaccagccga tcagcggttg gattgctatg gggtgtcaac tcaacagcaa cgcggctcat
     2581 tgtatccgct tcatccaaga ccggacgggt ttcgacgaac cgcccctcat tgaccaggaa
     2641 atcgagtcga tcctgatcag cggctgcacc gggcaaggcg gggtcatcgc caatggcgtc
     2701 atccgcgtgg acgacccggc catgatgatc tccgcccgca ttctggatat ggatctgaca
     2761 cactcgacgg cggaggttca cactggggac aaccgggacg ccatccgcct catcggcgga
     2821 aatgtgacca tcaccggcat cttgcgcagt cctgtccgaa gcgcgctgaa tgcctcggct
     2881 ggcacaacgg ggaccgtcga catagtcagt gacgacgccg gacaagttag cggctacccc
     2941 gccgtcctgc tcacgagtgt cggagagatg gtagttgatg gtcgatatca ggcctatgac
     3001 ttccaaggcg tgaaaatact caattgttcc gacgtcattc gcagccggtt cctcgacgtc
     3061 tataatatcg gcaatggatg ttccggcgtt gatgtcgttg gcagttcggc aggctacgac
     3121 gcaggtcacc tgaaggtaac ggccgaggcc ggcgcgacat cggcgcgagg cgttggcggc
     3181 gtcggcggtt cggtcctgcg catcggcgga ttttcgcccc gcggcgtgcg cccgttcggc
     3241 aacccaacca cgcccgcaag catcgcggct gcgttcctgc ctggcgagtt cagatcagcc
     3301 tacgtagctt tcggagctac caccacctac ggcgacggag tgctaaagct actgggtgag
     3361 ggcgacgcca ccgacgacct tctgaacatc acaaatggct atgtcggtca gcggctgacg
     3421 gtgatgaacg gcatggccga tccgaccacg acgacgatta ccattaagtc cacgggcaat
     3481 atcgcggtgg cttcaaaccg cgtgctcaat gccggctgga gccaagtcga attgctgtgg
     3541 aatggatcat cctggattga aacggcgttc tgggtctaac gcaaacgagt cgggcggcct
     3601 ggacgaccgg atccgccggg gccgcggccg tgtccggctt gaccggcgcg ccgcgatcgc
     3661 gctcgctgtc gacgccgatt tcgcgagacc gtcgaccgca tgaccccaga agccggagcc
     3721 aggccacc
//
LOCUS       DAORNX010000307         3725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18596_length_3725_cov_7.054223, whole genome shotgun sequence.
ACCESSION   DAORNX010000307 DAORNX010000000
VERSION     DAORNX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18596_length_3725_cov_7.054223"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLO65_12425"
     CDS             <1..631
                     /locus_tag="PLO65_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD29098.1"
                     /translation="LFANPDGRAAPLAAALAPLAERLAAIDPGRLVTAGVLTFESPWN
                     WKVMVENFIESYHHIGPHAASLQQTNPGLTTSATSDSSLFSILENPPASPDHGAFVVA
                     AIFPATLMFFTEGEAPFGVWYELSDIAPERFILTIHLLVAPELAAVPEIVEAFREQVA
                     AVHGEDIPVCAGVQKGVASRFYQPGPLSHLEAALWAFHRHLAGCFSDGA"
     gene            633..1871
                     /locus_tag="PLO65_12430"
     CDS             633..1871
                     /locus_tag="PLO65_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD29099.1"
                     /translation="MSPFHEVVIVGGGVGGSALAASLAGAGRDVLLLEQTEVYEDRVR
                     GEWIAPWGVAEVRRMGLLDVLRDAGGHYVTRHVTYDESRTPQEAEAATLPLGMFAADI
                     PGPLTLGNPHHCQTLFDEATRRGATTLRGAMVTAITPGAEPAVTYLLDDVEHTVRCRL
                     IVGADGRASPVREACGITLNQDRPHHWFAGMLVDQAPDWPDDLQAIGTEGDFGFLAFP
                     QGGGKVRLYGSYALEQKRRFAGPEGPRQFLDAFNVACSPVNQTLLAGAPAGPVLSFFN
                     NDTWTDQPFAEGVVLIGDAAGWNDPIVGLGQSITYRDARTVHEVLTGGGDWSPAAFAD
                     YGAERAERMRRLRFCAAITSNLDAEFGPEAKARRRRHFERSLADPSLGMHGFAIMAGP
                     ETLPPEVFTPEHRDRVLEQA"
     gene            1871..2221
                     /locus_tag="PLO65_12435"
     CDS             1871..2221
                     /locus_tag="PLO65_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD29100.1"
                     /translation="MDIEDFNRRWLAAWTAKDVEALIGFYAPDCTYKDPQTAAGIVGH
                     DALRGYLTGLFAATPPMTYEPETVWAIAGGFCGRWYCTINGPDGPTRMRGFDLVEFDG
                     DRIGWNEVYVHPLP"
     gene            2278..3138
                     /locus_tag="PLO65_12440"
     CDS             2278..3138
                     /locus_tag="PLO65_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRD29101.1"
                     /translation="MTRLTIHHETRYSYERPVRFSQHRLLLRPRDSHALRVIEARLSL
                     SPPGETRWVYDALGNCVCLFEPGGEARSLIIVSRLVTERFPAPLSPDDPGTGMPVAYG
                     LEDRTVLAPFIAPATEADGGALQEWLRSLTGAPGEPALDLLMRMTGAIHQGFEYRERQ
                     VAGVQTPGQTLRLRSGACRDFAWLMIEALRRLGFAARFVSGYVNGGGARRGAGATHAW
                     VDVFLPDLGWTEFDPTNGLAESADLIRIAVARTPREAAPVSGAILGDPGRAELTVGVM
                     VSSETAAVAA"
     gene            3470..>3725
                     /locus_tag="PLO65_12445"
     CDS             3470..>3725
                     /locus_tag="PLO65_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29102.1"
                     /translation="MRARTATAVFAVTALGAAGLGAATSMSAATDGLTSDSLMADARI
                     GDAFSGAGARVPDDPDAPITTGRWAENAAAVELAGGPGAGP"
BASE COUNT          530 a         1379 c         1274 g          542 t
ORIGIN      
        1 gctgttcgcc aacccggacg ggcgggccgc cccgctggcc gcggcgctgg cccccctggc
       61 agagcgactg gcggccatcg acccgggccg gctggtcacc gccggcgtcc tgaccttcga
      121 gtccccgtgg aactggaagg tgatggtcga gaacttcatc gagtcctacc accacatcgg
      181 tccgcacgcg gcgagcctgc agcagaccaa cccgggtctg accacctcgg cgacgagcga
      241 cagcagcctg ttcagcatcc tcgagaaccc gccggcctcg cccgaccatg gcgcgttcgt
      301 ggtggcggcg atcttcccgg cgactctgat gttcttcacc gagggcgagg cgcccttcgg
      361 ggtctggtac gaactgtcgg acatcgcgcc cgagcgcttc atcctgacga tccacctgct
      421 ggtcgcgccc gaactggcgg cggtccccga aatcgtcgag gccttccgcg aacaggtcgc
      481 ggcggtccac ggcgaggaca ttccggtctg cgccggcgtc cagaagggcg tcgccagccg
      541 gttctaccaa cccgggccgc tcagccatct cgaggccgcc ctgtgggcct ttcaccggca
      601 tctggccggc tgtttcagcg acggagcctg acatgtctcc cttccacgaa gtcgtgatcg
      661 tcggcggcgg cgtcggcgga tccgccctgg cggccagcct tgcgggagcc ggccgcgacg
      721 tgctgctgct ggaacagacc gaggtctatg aagaccgggt gcgcggcgag tggatcgcgc
      781 cctggggggt ggccgaggtg cgccgcatgg gcctgctcga cgtgctgcgc gacgccggcg
      841 gccactatgt cacgcgccat gtgacctacg acgagagccg cacgccgcag gaggccgagg
      901 ccgccaccct gccgctgggg atgttcgccg ccgacatccc cggcccgctc accctgggca
      961 acccgcacca ctgccagacc ctgttcgacg aggcgacccg ccgcggcgcg accacgttgc
     1021 gcggcgcgat ggtcaccgcg atcacgcccg gcgccgagcc ggcggtgacc tatctgctcg
     1081 atgacgtcga gcacacggtg cgctgccggc tgatcgtcgg ggccgacggc cgcgcctcgc
     1141 ccgtgcgcga ggcctgcggc atcacgttga accaggaccg tccgcaccac tggttcgccg
     1201 gcatgctggt cgaccaggcc ccagactggc cagacgatct gcaggccatc ggcacggagg
     1261 gcgacttcgg cttcctcgcc ttcccgcagg gcggcggcaa ggtgcggctc tacggtagct
     1321 acgccctgga gcagaagcgc cgcttcgccg gcccggaggg gccgcgccag ttcctcgacg
     1381 ccttcaacgt cgcctgcagt ccggtcaacc agacgcttct ggcgggagcg ccggccgggc
     1441 cggtgctgag cttcttcaac aacgacacct ggaccgacca gccgttcgcc gagggcgtgg
     1501 tgctgatcgg cgacgccgcg ggctggaacg acccgatcgt cggcctggga cagtcgatca
     1561 cctaccgcga cgcccgcacg gtgcacgagg tgctgaccgg cggcggcgac tggtcgccgg
     1621 ccgcctttgc cgactacggc gccgagcggg ccgagcggat gcgccgcctg cgcttctgcg
     1681 cggccatcac ctcgaacctc gacgccgagt tcgggccgga ggccaaggcc cgccgccgcc
     1741 ggcatttcga gcgcagcctg gctgacccct cgctcggcat gcacggcttc gcgatcatgg
     1801 ccgggcccga gaccctgccg cccgaggtct tcacccccga acaccgcgac cgcgtgctgg
     1861 agcaagcctg atggacatcg aagacttcaa ccgccgctgg ctggccgcct ggacggccaa
     1921 ggacgtcgag gccctgatcg gcttctacgc gccggactgc acctacaagg acccgcagac
     1981 ggccgccgga attgtcggtc atgacgccct gcgcggctat ctgaccggcc tgttcgcggc
     2041 cacaccgccg atgacctacg agccggaaac cgtttgggcc atcgccggcg gcttctgcgg
     2101 ccgctggtac tgtacgatca acggcccgga cggcccgacc cgcatgcgcg gcttcgacct
     2161 ggtcgaattc gacggcgacc gcatcggctg gaacgaggtc tacgtccacc ccctgccctg
     2221 accgttcagc ccttcggcat tggaacctcg gcccgggcgc cggggttcca agcccgcatg
     2281 acccgcctga cgatccacca cgagacccgc tacagctacg agcggcccgt ccggttcagc
     2341 cagcacaggc tgttgttgcg tccacgagac agccacgccc ttcgggtgat cgaggcccgg
     2401 ctgtcgctgt cgccgcccgg cgagacccgc tgggtctatg acgccctggg caattgcgtc
     2461 tgcctgttcg agcccggcgg cgaagcccgg tccctcatca tcgtcagccg gctggtgaca
     2521 gagcgctttc ccgccccgct ttcgcccgac gaccccggga ccggcatgcc ggtcgcctac
     2581 gggcttgagg accgcaccgt attggccccc ttcatcgctc cggcgaccga ggcggacggc
     2641 ggcgccctgc aggagtggct gaggtccctg acgggcgcgc cgggcgagcc ggcgctcgac
     2701 ctgctgatgc ggatgaccgg cgccattcac cagggctttg agtaccgcga gaggcaggtc
     2761 gcgggggtgc aaacgcccgg ccagaccctg cggctgcgga gcggagcctg ccgcgacttc
     2821 gcctggctga tgatcgaggc gttgcgccgc ctcggtttcg cggcccgttt cgtcagcggc
     2881 tatgtcaacg ggggcggcgc ccggcgcggc gcgggagcga cccacgcctg ggtcgatgtg
     2941 ttcctgccgg acctgggctg gaccgagttc gacccgacca acggcctggc ggaatcggcc
     3001 gacctgatcc gcatcgccgt cgcccgcacc cccagggagg cggcccccgt gagcggcgcg
     3061 atcctcggcg atcccggccg ggcggaactg acggtcggcg tgatggtttc gtccgagacg
     3121 gccgccgtcg cggcctgagc cccgatcggg caagtgtgag gcctgcgttc catgaccagc
     3181 ggatttcaac agcagaaccc gtgggcgact ttcaccccgc cgaagaccga cgccctgccc
     3241 cagcacggcc agaagcccga caaggcgtgc gttgcaccgc cgccgccgcg caagccaaag
     3301 tcccgtcctg cctcctgacg tgatcttgat caccggcgtc cggcctgaaa cgggcaatga
     3361 ttccaccgcc tgacaggggc gccgcccctc gcccgccccg cgagcggggt tgccagtcgc
     3421 gctttcctcg ttaacctttg gtttgcaggt caaacggggg cagcgcttga tgcgcgctag
     3481 aacggccaca gcagtgttcg cggtgacggc cctgggggcc gctggcctcg gcgcggccac
     3541 cagcatgagc gcggccaccg atggactgac ctcggactcc ctgatggcgg acgcccgcat
     3601 cggcgatgcg ttcagcggcg caggcgcccg cgtcccggac gatccggacg cgccgatcac
     3661 gacgggccgc tgggcggaga acgccgcggc agtggagctg gccggcgggc ccggagcggg
     3721 gccgg
//
LOCUS       DAORNX010000308         3720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18621_length_3720_cov_7.801637, whole genome shotgun sequence.
ACCESSION   DAORNX010000308 DAORNX010000000
VERSION     DAORNX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_3720_cov_7.801637"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLO65_12450"
     CDS             <1..695
                     /locus_tag="PLO65_12450"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRD29103.1"
                     /translation="APPEAARAAMDALAAGSFAAYRGLVYETPGFTDFFWSATPIGEI
                     VQLNIGSRPASRTASRRIEDLRAIPWVFSWSQARFMLPGWFGFAGGVARAGLAVPQLT
                     ELAGEFDVFATLLSNMELALAQSDMAIAARYAGLAEDQTAARTIMDTIRAEHDRAVEI
                     AVAVRGGTRLLDDRPDLQESVELAAQAVDPLNYLQLELLSRRRAGAGDDQLRLAIQLT
                     VAGIAAGLRNTG"
     gene            825..1973
                     /locus_tag="PLO65_12455"
     CDS             825..1973
                     /locus_tag="PLO65_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29104.1"
                     /translation="MALDAETRDQFIDTLRRFVTERLRPLEAQVAADDKVPASIIREM
                     ADLGLFGISIPEEYGGLDLSMEDECLVVFELGRTSPAFRSEIGTNVGIGSQGLVMFGT
                     DEQKAKWLPGIASGEIVTSFALTEPEAGSDSASVQTRATFDGADYVLNGSKRYITNAG
                     KAGLFTVMARTNPDVKGGGGVSAFLVPSDLPGITVGKPEKKMGQHGAQIHDITFDNVR
                     VPAWNRLGGEGEGFKVAMQVLDKGRLHIAALCVGVAERLIADSVAYAGERKQFGQALA
                     QFQLIQGMIADMKTEAMAARALVMEGARKRDRGETIVLEAAAAKYFASEMVGRVADKA
                     VQIFGGAGYVADYGIERLYRDVRIFRIYEGTSQVQQIVIARETMKRGG"
     gene            complement(2016..2747)
                     /locus_tag="PLO65_12460"
     CDS             complement(2016..2747)
                     /locus_tag="PLO65_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD29105.1"
                     /translation="MRKFILMAGAATALVGAPAMAQDGGAYVSVSAGLASVKDTELRY
                     YDAGGTFGGVGATDSVQTSVEMKNAAAFAGTAGYDFGVVRAELEIGYNRNELEAITVK
                     SVNGAPVTLTPADVADVCLYLDIGGCTATGNRIAFSDGGRMRQLSAMANLWFDIPVSD
                     RVTPYVGGGAGLLGFEADGEGDATFAWQLGAGVAFQASPNLAITADYRYRSADGKDIA
                     YDSVSGFEIGRMKTSILSAGLRYSF"
     gene            2936..>3720
                     /locus_tag="PLO65_12465"
     CDS             2936..>3720
                     /locus_tag="PLO65_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29106.1"
                     /translation="MPVAGNRIDPSALIDELRAGRVTAWIGVLLLASVVAYLSVTFAD
                     AVSPPVLGLWSGAFALVLLVWCGAMAMAMTRDPGEREILRIWIPLFKGVAIAANLLIA
                     VSVWLFLPVAGPELFALMLVMYVWYIPIQMIAENRATRVSVLATLMVLGSLVAFVMTH
                     PAPYSTALGVFLLVFAVSLLAMRRVIHRAVAEAIMAKLEAERAGQALNAALSEVADQR
                     DAIARFLAAASHDLQQPVQAARLFFDQAIDTADARARDRAVHGA"
BASE COUNT          553 a         1292 c         1288 g          587 t
ORIGIN      
        1 cggccccgcc ggaggccgcg cgggcggcga tggacgccct ggccgccggc tcgttcgccg
       61 cctatcgcgg cctggtctac gagaccccgg ggttcaccga cttcttctgg tcggccaccc
      121 cgatcggcga gatcgtccag ctgaacatcg gcagccgccc ggcctcgcgc accgccagcc
      181 ggcggatcga ggacctgcgg gccatcccct gggtgttcag ctggtcgcag gcgcgcttca
      241 tgttgcccgg ctggttcggc ttcgccggcg gcgtggcgcg cgccggcctg gccgtgcccc
      301 agctgaccga gctggccggc gagttcgacg tcttcgccac cctgctgtcg aacatggagc
      361 tggccctggc ccagagcgac atggccatcg ccgcccgcta cgccggcctg gccgaggacc
      421 agacggcggc gcggaccatc atggacacga tccgggccga gcatgaccgc gccgtcgaga
      481 tcgccgtggc cgtccgcgga ggaacccggc tgctggacga ccggcccgat ctgcaggaat
      541 ccgtcgagct ggccgcccag gcggttgatc cgctgaacta cctgcagctg gagttgctgt
      601 cgcgccgccg ggcgggggcg ggtgacgacc agctgcggct ggccatccag ttgaccgtcg
      661 ccggcattgc ggcagggttg aggaacacgg ggtaggcggc ggggacatgt accgaaggta
      721 cgtgtcccct gctcgtgtcg ctcgggtggg ggacacgtac ccttgcgggc acatgtcccc
      781 tcccttactg catctggaaa caggacgcat aagggaagca tcgcatggcc ctcgacgccg
      841 agacccggga ccagttcatc gacaccctgc gccggttcgt gaccgagcgg ctgcggccgc
      901 tggaggccca ggtggcggcg gacgacaagg tgcccgccag catcatccgg gagatggcgg
      961 acctcggcct gttcggcatc tcgatccccg aggaatacgg cggcctggac ctgtcgatgg
     1021 aagacgagtg cctggtggtg ttcgagctgg gccgcaccag cccggcgttc cgctcggaga
     1081 tcggcaccaa cgtcggcatc ggcagccagg gtctggtgat gttcggcacc gacgagcaga
     1141 aggccaaatg gctgcccggc atcgcctcgg gcgagatcgt cacctccttc gccctgaccg
     1201 agccggaagc cggctccgac agcgcctcgg tgcagacccg cgccacgttc gacggcgccg
     1261 actacgtgct gaacggctcc aagcgctaca tcaccaacgc cggcaaggcc gggctgttca
     1321 cggtcatggc ccgcaccaat cctgacgtga agggcggcgg cggggtctcg gccttcctgg
     1381 tgcccagcga tctgccgggc atcacggtcg gcaagccgga aaagaagatg ggccagcacg
     1441 gcgcccagat ccacgacatc accttcgaca acgtccgcgt tccggcctgg aaccgcctgg
     1501 gcggcgaggg cgagggcttc aaggtggcca tgcaggtgct cgacaagggt cgcctgcaca
     1561 tcgccgccct ctgcgtgggg gtggccgagc gcctcatcgc cgacagtgtc gcctacgccg
     1621 gcgagcgcaa gcagtttggc caggccctgg cccagttcca gctgatccag gggatgatcg
     1681 ccgacatgaa gaccgaggcc atggccgccc gcgccctggt catggagggc gcgcgcaagc
     1741 gcgatcgtgg cgaaaccatc gtcctggaag cggcggccgc caagtacttc gcctcggaga
     1801 tggtcggccg ggtcgccgac aaggcggtgc agatcttcgg cggcgccggc tatgtcgctg
     1861 actacggcat cgagcgcctg taccgcgacg tgcgcatctt ccgcatctac gagggcacca
     1921 gccaggtgca gcagatcgtc atcgcccgcg agacgatgaa gcgcggtggg taggatggat
     1981 cggccgcgcg ggcgggggcc cgcgcggccg gacggtcaga agctgtagcg aagcccggcc
     2041 gaaagaatgc tggttttcat ccggccgatc tcgaagcccg agaccgaatc gtaggcgatg
     2101 tccttgccat ccgccgaccg gtagcggtag tcggccgtga tcgcgaggtt cgggctggcc
     2161 tggaaggcca ccccggcccc cagttgccag gcgaaggtgg cgtcgccttc gccatcggcc
     2221 tcaaagccca ggaggcccgc gccaccgccg acatacggcg tcacccggtc gctgaccggg
     2281 atgtcgaacc acaggttggc catggcgctc agctggcgca tgcgcccgcc gtcgctgaag
     2341 gcgatgcggt tgccggtggc ggtgcagccg ccgatgtcca gatagaggca gacgtcggcg
     2401 acgtccgccg gggtcagggt caccggcgcg ccgttgaccg acttgaccgt gatggcttcc
     2461 agttcgttgc gattgtagcc gatctccagc tccgcgcgca cgaccccgaa gtcatagccg
     2521 gcggttcccg cgaaggccgc cgcgttcttc atctccaccg aggtctgcac ggaatcggtc
     2581 gcgccgacgc cgccgaaggt cccgccggcg tcataatagc ggagctccgt gtccttcacc
     2641 gaggcgaggc ccgccgaaac actgacgtag gctcccccgt cctgggccat cgccggcgcc
     2701 ccgaccaggg ccgtggcggc gcccgccatg agaatgaact ttcgcattgc tcccctcctg
     2761 ccgccactcg actaatccgg gcgcggtcgg cgcggcgcga ccgggccagg ttgctgctgc
     2821 ggccggtgaa aaccggcgct tgctagaggt cgaaactact gtagggattt ggcggcggca
     2881 tcggccggct ggccgaaccg tttgggtgaa cctgtagttc aggcgaagcg taacaatgcc
     2941 ggtcgcaggc aacaggatcg acccgtcggc gctcatcgac gagctgcggg ccgggcgggt
     3001 gacggcctgg atcggcgtcc tgctgctggc ctcggtggtc gcctacctct cggtgacctt
     3061 cgccgacgcg gtgagcccgc ccgtccttgg tctgtggtcc ggcgcattcg ccctggtcct
     3121 gctcgtctgg tgcggagcca tggcgatggc catgacgcgc gaccccggcg aacgggaaat
     3181 cctgcggatc tggattccgt tgttcaaggg cgtggccatc gccgccaacc tgctgatcgc
     3241 cgtcagtgtc tggctgtttc ttcccgtcgc cgggccggaa ttgttcgccc tcatgctggt
     3301 gatgtacgtc tggtacatcc cgatccagat gatcgccgag aaccgcgcca cccgggtttc
     3361 ggtcctggcg accctgatgg tgctcgggtc gctggtcgcg ttcgtgatga cccatccggc
     3421 gccctacagc acggcgctcg gcgtcttcct gctggtgttc gccgtcagcc tcctggccat
     3481 gaggcgggtg atccaccgcg ccgtcgccga ggcgatcatg gccaagctgg aggccgaacg
     3541 cgccggccag gcgctgaacg ccgcgctgtc ggaggtcgcc gaccagcgcg acgccatcgc
     3601 ccggttcctc gccgccgctt cccacgatct gcagcagccg gtccaggccg cgcggctgtt
     3661 cttcgaccag gcgatcgaca ccgccgacgc ccgggcccgg gatcgcgccg tccacggcgc
//
LOCUS       DAORNX010000309         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18641_length_3718_cov_5.992083, whole genome shotgun sequence.
ACCESSION   DAORNX010000309 DAORNX010000000
VERSION     DAORNX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18641_length_3718_cov_5.992083"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1025)
                     /gene="trxB"
                     /locus_tag="PLO65_12470"
     CDS             complement(18..1025)
                     /gene="trxB"
                     /locus_tag="PLO65_12470"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRD29107.1"
                     /translation="MSTSAPRTTRCLIIGSGPAGWTAAIYAARALLKPVLIQGIQPGG
                     QLTITTDVENYPGFADVIQGPWLMEQMEAQARHVGTEIVNDIVIEADLSQRPFRIRTD
                     SGQDWLAETLIIATGAQAKWLGLDSEARFQGFGVSACATCDGFFYRGKEVIVVGGGNT
                     AVEEALFLTSFASKVTVVHRKAEFRAEKILQERLFANPKIEIIWDHAIDEVLGETDPM
                     GVTGVRLKNVKTGETQTVKADGVFIAIGHAPSSELFRDQLETHAGGYLSVKPGTTSTA
                     IPGVYAAGDVTDDIYRQAVTAAGMGCMAALEAARFLAEQDHAAAQQPVGQGEAARIGA
                     W"
     gene            1086..1370
                     /locus_tag="PLO65_12475"
     CDS             1086..1370
                     /locus_tag="PLO65_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29108.1"
                     /translation="MAGNSRIGVGVALGVAIGAGLGVALHNVALGIGLGVAIGVALGL
                     AMSAKAGRGKGDGAGSGAEGWSGDGGGHDRDHHDGGSDGGGDGGGDGGGD"
     gene            1423..2478
                     /gene="pseI"
                     /locus_tag="PLO65_12480"
     CDS             1423..2478
                     /gene="pseI"
                     /locus_tag="PLO65_12480"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HRD29109.1"
                     /translation="MPPVPEIAIAGRRIGPGHPPYVICELSGNHNGSLERALTMIDAA
                     AATGCDAIKIQTYTADTITLDVDRPEFVIHGGLWDGQTLHALYREAQTPYEWHAALFA
                     RAAERGVTLFSSPFDETAVDLLAGLDAPAYKIASFEAVDLPLIRYAAARGKPLIISTG
                     MANLAEMEAARDAALAGGAAGVVMLHCVSSYPATFEDANVRTIPDMAARLGVAVGLSD
                     HTPGTATAVAAVALGACVIEKHFTLARSDGGPDAAFSLEPAEFTALVRDCRDAWAALG
                     QAHYDVLGSEKANRQFRRSLYVTAPVRAGEPLTRANVRSVRPGNGLPPADLDRVLGRT
                     ASRDLAFGEPLDWSMVS"
     gene            complement(2475..3341)
                     /locus_tag="PLO65_12485"
     CDS             complement(2475..3341)
                     /locus_tag="PLO65_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29110.1"
                     /translation="MTIERKSVVVTGVSTGIGWGATRVLIDKGFHVFGSVRKAADGER
                     LEAECGDAFTPLLFDVVDEAAVRAAARQVEAALGGRTLAGLVNNAGIAVAGPLLHLPV
                     EEFRKQMEVNVTGIVTVTQAFAPLLGAESPARKDPGRIVNISSVAGRTGNPFMAPYSA
                     SKFAVEGLSEALRRELLPFGVDVVVIAPGAVATPIWSKAEEQDITPYANTVYADALKR
                     LQAYMLGLGAKGLPPQRIGEAIHTALTTPRPKVRYTLSPDPFQVFMSERVLSKRGLDR
                     IVGKRLGLMPQT"
     gene            3455..>3718
                     /locus_tag="PLO65_12490"
     CDS             3455..>3718
                     /locus_tag="PLO65_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HRD29111.1"
                     /translation="MTQPRILFVCDAGPGVGGGHVMRCLTLAGALAARGAACAFVRTP
                     EAADILARYAPDMPLVDEDAATDLVVLDSYRMAPATEAAWRARA"
BASE COUNT          524 a         1273 c         1367 g          554 t
ORIGIN      
        1 cggcgcggcc gggcggatca ccatgcgccg atcctggccg cctccccctg gccgaccggc
       61 tgttgggccg cggcgtggtc ctgctcggcc aggaagcgcg cggcctccag cgcggccatg
      121 cagcccatgc cggccgcggt cacggcctgg cggtagatgt cgtctgtcac gtcgccggcg
      181 gcgtagaccc ccggaatggc cgtcgaggtg gtccccggtt tgaccgacag atagccgccg
      241 gcgtgggtct cgagctggtc cctgaacagc tccgacgagg gggcatggcc gatggcgatg
      301 aacaccccgt cggccttgac ggtctgcgtc tcgccggtct tcacgttctt cagccgcacg
      361 cccgtcacgc ccatcggatc ggtctcgccg agcacctcgt cgatggcgtg gtcccagatg
      421 atctcgatct tcgggttggc gaacagccgc tcctgcagga tcttctcggc gcggaactcg
      481 gccttgcggt ggaccacggt gaccttgctg gcgaagctgg tcaggaacag ggcctcctcc
      541 acggcggtgt ttccgccgcc cacgacgatg acctccttgc cgcgatagaa gaagccgtcg
      601 caggtggcgc aggcgctgac cccgaagccc tggaacctgg cctcgctgtc gaggcccagc
      661 cacttggcct gggcgccagt ggcgatgatc agcgtctcgg ccagccagtc ctggccgctg
      721 tcggtgcgga tgcggaacgg ccgttgcgac aggtcggcct cgatgacgat gtcgttgacg
      781 atctcggtcc cgacgtggcg cgcctgggcc tccatctgct ccatcagcca ggggccctgg
      841 atgacgtcgg cgaagcccgg atagttctcc acgtcggtgg tgatggtcag ctggccgccg
      901 ggctggatgc cctggatcag caccggcttg agcagtgcgc gggctgcata gatggcggcg
      961 gtccagccgg cggggccgga accgatgatc aggcagcggg tggtgcgggg ggcggaggtc
     1021 gacatgggct ccaatttagg gatgattccg tcccggcgcg atggccgcgg gcgagaggcg
     1081 acgaaatggc cggcaacagc aggatcgggg tgggtgtcgc cctgggcgtg gcgatcggcg
     1141 ccggccttgg cgtggcgctg cacaacgtcg ccctgggcat cggcctcggg gtcgccatcg
     1201 gcgtcgccct gggcctggcg atgtcggcga aggcagggcg gggcaagggc gacggcgccg
     1261 ggtcaggcgc agaagggtgg agtggcgacg gcggcggaca tgaccgcgat caccacgacg
     1321 gcggctctga cggcggcgga gacggcggcg gcgacggcgg cggcgactga cgctcgcgaa
     1381 cgggtctagg atggcgccga ctcagtccgt ccgagccccg ccatgccgcc agtcccggaa
     1441 atcgccatcg ccggccgcag gatcggcccc ggccatccgc cctatgtcat ctgcgagctg
     1501 tcgggcaatc acaacggcag cctcgagcgg gcgctgacga tgatcgacgc ggcggccgcg
     1561 accggctgcg acgcgatcaa gatccagacc tacaccgccg acaccatcac cctcgacgtc
     1621 gaccggccgg agttcgtcat ccatggcggg ctgtgggacg gccagaccct gcacgccctc
     1681 tatcgcgagg cgcagacgcc gtatgagtgg cacgcggcgc tgttcgcgcg ggcggccgag
     1741 cggggggtga cgctgttctc cagcccgttc gacgagacgg ccgtcgacct gctcgccgga
     1801 ctggacgcgc cggcctacaa gatcgccagc ttcgaggctg tcgacctgcc gctgatccgc
     1861 tacgcggcgg cgcgcggcaa gccgctgatc atctccaccg gcatggccaa cctggccgag
     1921 atggaggcgg cccgcgacgc ggccctggcc ggcggagccg cgggggtggt catgctccac
     1981 tgcgtctcca gctacccggc gaccttcgag gacgccaacg tgcggacaat ccccgacatg
     2041 gccgctcggc tgggcgtggc ggtcggcctg tcggatcaca cccccggaac ggccacggcc
     2101 gtggcggcgg tggccctggg cgcctgcgtg atcgaaaaac atttcaccct ggcccggtcc
     2161 gacggcgggc cggacgcggc cttcagcctc gagcccgccg agttcaccgc cctggtgcgc
     2221 gactgccgcg acgcctgggc ggcgctgggc caggcccact acgacgtgct gggctcggag
     2281 aaggccaacc gccagttccg ccggtcgctg tacgtcaccg cgccggtcag ggccggcgag
     2341 ccgctgaccc gggccaacgt gcggtccgtg cggccgggca acggtctgcc gccggcggat
     2401 ctggacaggg tgctggggcg gacggccagc cgcgacctgg cgttcggcga accgctggac
     2461 tggtcgatgg tcagctaggt ttgcggcatc agccccagcc gcttgccgac gatcctgtcg
     2521 agaccgcgct tggacagcac ccgctcggac atgaacacct ggaagggatc cggcgagagg
     2581 gtgtagcgga ccttcggccg gggcgtggtc agggcggtgt ggatcgcctc gccgatgcgc
     2641 tggggtggca gccccttggc cccgaggccg agcatatagg cctgaagccg cttcaacgcg
     2701 tcggcgtaga ccgtattggc gtagggggtg atgtcctgtt cctcggcctt gctccagatc
     2761 ggcgtggcca cggcgccggg ggcgatgacg accacatcga cgccgaacgg cagcagctcg
     2821 cggcgcagcg cctctgacag gccttcgacg gcgaacttcg aggccgaata gggagccatg
     2881 aacgggttgc cggtgcgacc ggccaccgag gagatgttga cgatccggcc ggggtccttg
     2941 cgggcggggc tttcggcgcc gagcagcggg gcgaaggcct gggtcacggt gacgatgccg
     3001 gtgacattga cctccatctg cttgcggaac tcctcgaccg gcaggtgcag cagcgggccg
     3061 gcgacggcga tgccggcgtt gttgaccagg cccgcaaggg tcctgccgcc gagcgccgcc
     3121 tcgacctgac gcgcggcggc gcggacggcg gcttcgtcga cgacatcgaa cagcagcggc
     3181 gtgaaggcgt cgccgcactc ggcttccagc cgctcgccgt cggccgcctt gcggacactg
     3241 ccgaagacat ggaagccctt gtcgatgagc accctggtcg cgccccagcc gatgcccgtg
     3301 gagacgccgg tgacgacgac ggatttcctc tcgatggtca tggacgcccc ccaagtgcgt
     3361 tcgctttgag gacgatatgg ggccatcgcc ctgcgttcgc cagtggaagt ccgatatggc
     3421 ttaagctgcc gcgactcacc tggaccggcc gccgatgacc cagccccgca tcctgttcgt
     3481 ttgcgacgcc ggcccgggcg tgggcggcgg acatgtgatg cgctgcctga ccctggccgg
     3541 ggcgctggcg gcgaggggcg cggcctgcgc cttcgtccgc acacccgagg ccgccgacat
     3601 cctcgcccgc tatgcgccgg acatgccgct ggtcgacgag gacgccgcga ccgacctcgt
     3661 ggttctggat tcctaccgca tggcgccggc gacggaggcg gcctggcggg cgcgggcg
//
LOCUS       DAORNX010000310         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18642_length_3718_cov_5.503140, whole genome shotgun sequence.
ACCESSION   DAORNX010000310 DAORNX010000000
VERSION     DAORNX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18642_length_3718_cov_5.503140"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>327
                     /locus_tag="PLO65_12495"
     CDS             <1..>327
                     /locus_tag="PLO65_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRD29112.1"
                     /translation="RAFAAFAAARAGRTVWLVDLDLFTSPQHAALVASPEIYGPLGKA
                     VSATPADGATFFTVQPPTLMPDRSAAPDSRFLVAHAVGRHRWWVTRFRADVLRGSQTV
                     HVLPSAD"
     assembly_gap    328..344
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <345..593
                     /locus_tag="PLO65_12500"
     CDS             <345..593
                     /locus_tag="PLO65_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRD29113.1"
                     /translation="NALKRHADLVVVDCPSADRSQAALTVAPFMDQTVLVVAADQPDV
                     QAPARLRDGLAAAGGRVSGVFFNRAQVEAPGFLRAFLP"
     gene            590..1843
                     /locus_tag="PLO65_12505"
     CDS             590..1843
                     /locus_tag="PLO65_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRD29114.1"
                     /translation="MTVQAPAWPAPEARRTDLWDWLAFGLAVFLVLIYSQGWIVPIFG
                     AKIDAGSSGAIRNVYFPAYAAGIGLLAMVPGATVAAMIRQPFLLLLLLIVALSTLWSI
                     APDQTVRRIIAVYATTLGGVVLATRFRWAGMAEVLAVANALLVIGSFAWAILPPHKGI
                     MTELFPGAWRGLWTEKNALGGDMAMAFGIFAAAALLNPRRRVLWWSFAGLALALVLLS
                     TSKTSLVSLILGAGTVGFVWFARRGPVSGVAATWAAVLGALLLAGFLLLASDVFLQLL
                     GKDATLTGRTKIWAAALRQAELRPWTGYGYAAVWDDKSGWGPLAWITREAGFKPSHAH
                     NAWLEQWLGLGYGGLLAWILFYLQTMAAAIASVFSRPAAYLALPYLVIYSLLTLTESV
                     AVTYNDLRWSIFVALAVKLAWRGRD"
     gene            complement(1815..2741)
                     /locus_tag="PLO65_12510"
     CDS             complement(1815..2741)
                     /locus_tag="PLO65_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRD29115.1"
                     /translation="MTTMFERMGVPIELGDRRFLHPGPLRWLRATAWMIGLFLLVALA
                     GYLPLGLMERFVGKGEPGELLINVVAALLALAIYVLMVWLAEGRRASELAPRPAIGGV
                     LAGFAIGMAVFGAAMIVLIGSGLYQIAWLGPAPAWTGAALSVQSGVIEEIMARAVILR
                     LTWRAFGPWVAFIFSAGLFGALHLGNDNASAWAALCIAVEAGILLGAFYALTGRLWVS
                     IGLHAGWNFTQGYLFGAAVSGTDFGGAMARSTARDGLPLWLTGGPFGPEASLPALVIC
                     SAAGVLTLAAAWKAGRFARTAPVASPDPARPA"
     gene            complement(2835..3332)
                     /locus_tag="PLO65_12515"
     CDS             complement(2835..3332)
                     /locus_tag="PLO65_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD29116.1"
                     /translation="MRETAQRPKSSGVQAQGCCACGAVRFEIDVPAVWAWHDHGEASR
                     RIMGGAYATWVGSWRSRFRWLAGENQLTRYEDAQARTTRSFCGRCGTPVLYERAGSPR
                     MVNIPRGLFAGRTGREPRYHMNLGERADWTWAGEPLGPLKGYPGVMVQRARRRPRSLT
                     PDDIL"
     gene            complement(3526..>3718)
                     /locus_tag="PLO65_12520"
     CDS             complement(3526..>3718)
                     /locus_tag="PLO65_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRD29117.1"
                     /translation="AALRWLDRAAQRRAWLILYTHDVAPTPSAWGCTPEALGHLADAA
                     RAGGFDIVTAAEGARRLGL"
BASE COUNT          498 a         1340 c         1313 g          550 t
ORIGIN      
        1 cgggccttcg cggccttcgc cgcggcgcgc gccggccgca cggtctggct ggtcgacctg
       61 gacctgttca cctcgcccca gcacgcggcg ctcgtcgcat cgccggagat ctacggcccg
      121 ctgggcaagg ctgtttcggc cactcccgcg gacggggcga ccttcttcac ggtccagccg
      181 ccgacgctga tgccggatcg gtcggcggcg ccggattcgc gctttctcgt cgcccacgcg
      241 gtcggccggc accgctggtg ggtgacgcgc tttcgggccg atgtcctgcg cggcagccag
      301 acggtccatg tcctgcccag cgccgacnnn nnnnnnnnnn nnnnaacgcg ctgaagcggc
      361 acgccgatct ggtggttgtc gactgtccgt cggccgaccg gtcgcaggcg gccctcaccg
      421 tggccccgtt catggaccag accgtgctgg tggtcgccgc cgaccagcct gacgtccagg
      481 ctccggcccg cctgcgcgat ggcctggcgg ccgccggcgg gcgggtctcg ggggtgttct
      541 tcaaccgggc ccaggtcgag gcgccgggat tcctgcgggc cttcctgccg tgaccgtcca
      601 ggccccggcg tggccggcgc ccgaggcgcg gcggaccgac ctgtgggact ggctggcgtt
      661 cggcctggcc gtgtttctgg tgctgatcta cagccagggc tggatcgtgc cgatcttcgg
      721 ggcgaagatc gacgccggca gttccggcgc catccgcaac gtctatttcc ccgcctacgc
      781 ggccggcatc ggtctgctgg cgatggtccc cggggcgacc gtggcggcga tgatccgcca
      841 gccgttcctg ctgctgctgc tgctgatcgt cgccctgtcg accctctggt cgatcgcgcc
      901 cgaccagacc gtgcgccgga tcatcgccgt ctacgccacc accctgggcg gggtggtgct
      961 ggcgacgcgg tttcgctggg ccgggatggc cgaggttctg gcggtggcca acgccctgct
     1021 ggtgatcggc tcgttcgcct gggcgatcct gccgccgcac aaggggatca tgaccgagct
     1081 gttccccggc gcctggcgcg gcctgtggac ggagaagaac gcgctcggcg gcgacatggc
     1141 catggccttc ggcatcttcg ccgccgcggc cctgctcaac ccccgccggc gggtgctttg
     1201 gtggagcttc gccggtctgg ccctcgccct ggtgctgctg tcgacctcga agacctcgct
     1261 ggtgtcgctg atcctcgggg cggggacggt cgggttcgtc tggttcgccc ggcgcggtcc
     1321 ggtctccggc gtggccgcca cctgggccgc cgtgctcggc gccctgctgc tggccggatt
     1381 cctgctgctg gcctcggatg tgttcctgca gctgctcggc aaggacgcga ccctgaccgg
     1441 ccgcaccaag atctgggcgg cggccctgcg ccaggcggag ctccggccgt ggacgggcta
     1501 cggctacgcc gcggtatggg acgacaagag cggctggggg ccgttggcct ggatcaccag
     1561 ggaggccggg ttcaagccga gccacgccca caacgcctgg ctggagcagt ggctgggcct
     1621 gggctacggc gggctgctgg cctggatcct gttctatctg cagaccatgg cggcggcgat
     1681 cgcctcggtg ttcagccggc cggcggccta cctggccttg ccctatctgg tcatctactc
     1741 gctgctgacc ctgaccgaga gcgtggcggt gacgtacaac gatctgcgct ggtcgatctt
     1801 cgtcgccctg gccgtcaagc tggcctggcg gggtcgggac tagccacggg cgccgtccgg
     1861 gcgaagcgac cggccttcca ggcggcggcg agggtcagga cccccgcggc cgaacagatc
     1921 accagggccg gcagggaggc ctcgggcccg aacggaccgc cggtcagcca caggggcagg
     1981 ccatcccgcg ccgtgctgcg ggccatcgcg ccaccgaagt ctgtgcccga cacggcggcg
     2041 ccgaacaggt agccctgggt gaagttccag ccggcgtgca ggccgatcga cacccacagc
     2101 cggccggtca gggcgtagaa ggcgcccagc aggatcccgg cctcgaccgc gatgcacagg
     2161 gccgcccagg ccgaggcgtt gtcattgccc agatgcaggg cgccgaaaag gccggcggag
     2221 aagatgaagg ccacccaggg tccgaaggcc cgccaggtca ggcgcaggat cacggcgcgg
     2281 gccatgatct cctcgatcac gccggactgc accgacaggg ccgcgccggt ccaggccggg
     2341 gcggggccga gccaggcgat ctggtagagg ccgctcccga tcagcacgat catcgccgcg
     2401 ccgaagaccg ccatgccgat cgcgaagccc gccagcacgc cgccgatggc ggggcgaggg
     2461 gccagttcac tcgcccgtcg tccttcggcc agccagacca tcagcacata gatcgccagg
     2521 gcgagcaggg ccgccaccac gttgatcagc agctcgcccg gttcgccctt gccgacgaac
     2581 cgttccatca gccccagcgg caggtaacct gccagcgcca cgagcaagaa cagtccgatc
     2641 atccaggccg tggcccgcag ccagcgcagc ggtccgggat gcaggaagcg ccggtcgccc
     2701 agctcgatcg gcacgcccat gcgctcgaac atcgtcgtca tgaaaagtcc cccgagtccc
     2761 ccgagccgcc ggcggccccg gacaatctgc acggcgcggc ggcggccgtc acgttagaga
     2821 taggtcatct ccggctacag gatatcgtcc ggcgtcaggc tccggggccg gcgacgcgcg
     2881 cgttggacca tcacgcccgg atagcccttc agcgggccca gcggttcacc ggcccaggtc
     2941 cagtccgccc gctcgcccag attcatatgg tagcgcggct cgcggcccgt gcggccggcg
     3001 aacagcccgc gcgggatgtt gaccatcctt ggcgacccgg cgcgctcata gagcaccggc
     3061 gttccgcagc gtccgcagaa gctgcgcgtc gtccgggcct gggcatcctc gtaccgggtc
     3121 agctggttct cgcccgccaa ccagcggaac cggctgcgcc agctgccgac ccaggtcgca
     3181 taggcgccgc ccatgatccg ccggctcgcc tcgccatggt catgccaggc ccagacagcc
     3241 ggcacatcga tctcgaaccg cacggcgccg caggcgcagc agccctgggc ctgaacaccg
     3301 gaggatttcg gtctctgggc agtttcgcgc atggacggtc tcaagcggat gcgttcgccg
     3361 actaaagggc gttcgggggc cgctggctac tgacagcgac tgccatccgg gccgtgcgtc
     3421 cttcgacacg cccactgcgt gggctgctca ggatgacgtg cattggctct acgtcatggt
     3481 gagcagcgct cgaagagcgc gtgtcgaacc acgcaccaac gccccctaca gccccaggcg
     3541 gcgcgcccct tccgcggcgg tgacgatatc aaagccgccc gccctggcgg cgtcggccag
     3601 gtggccgagg gcctcgggcg tgcagcccca ggccgagggc gtgggggcga cgtcgtgggt
     3661 gtagaggatc agccaggccc ggcgctgggc ggcgcggtcg agccagcgca gggccgcg
//
LOCUS       DAORNX010000311         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18704_length_3710_cov_7.641313, whole genome shotgun sequence.
ACCESSION   DAORNX010000311 DAORNX010000000
VERSION     DAORNX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_3710_cov_7.641313"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PLO65_12525"
     CDS             complement(<1..1668)
                     /locus_tag="PLO65_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRD29118.1"
                     /translation="MSDRSTGQAAHRLVAVLGPTNTGKTHLAVERMLGHHSGMIGLPL
                     RLLAREIYDRVVRLKGPREVALITGEEKIVPPRPQYFVCTVEAMPLGREVDFLAVDEI
                     QLCADPERGHVFTHRLLHARGKSETMLLGAGTMAPLVRRLLPGVEIVERERFSQLRYT
                     GPKKLTRLPRRSAVVAFSADNVYAIAELIRRQRGGAAVVMGSLSPRTRNAQVALYQSG
                     EVDFLVATDAIGMGLNMDVDHVAFAGLRKFDGRKTRWLHPQEVGQIAGRAGRHVKDGT
                     FGVTGEAGEMDEDLIAAVEEHTFQPVAGAQWRNHRLDFANLPSLIRSLDATPNREGLA
                     LSAEALDEQALKRLAEDADVVHRVRDRVALERLWEVCQTPDFRKQTSDEHLRLIRTLF
                     DNLTTGRRRMPEDWMAGQFRGLDRTDGEIDTLATRLAGVRTLAYVANRPNWLADPAHW
                     QGVTRGLEDRLSDALHEKLMARFIDRRTSVLMRQLGDRAEMLAGVAEDGTVTVEGHVV
                     GRLTGLAFEAPRAGSSLEEKALRGAAQRAVGPEVARRLGIGRSTLYRK"
     gene            complement(1816..2439)
                     /locus_tag="PLO65_12530"
     CDS             complement(1816..2439)
                     /locus_tag="PLO65_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD29119.1"
                     /translation="MTGDTFYHEGHRALQDAFDSRRMADRLDVVRRTEFSDDDRAFIE
                     GAVYFFLATADAAGAPDCSFKGGPAGFVRVTGPSELAFPDYDGNGMFKSLGNVVVNPN
                     VGLLFIALHGAPKRLRVNGTARIDRNDPLLASTVGAQMIVRVEARAIFPNCPRYIPTL
                     ELAGMSPYTPAPGVEPVEPAWKGFDVFRDHVHPRQPTFKGQGVGSRE"
     gene            complement(2527..>3710)
                     /locus_tag="PLO65_12535"
     CDS             complement(2527..>3710)
                     /locus_tag="PLO65_12535"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRD29120.1"
                     /translation="ADGHAVVLNTAALKLAGITRDTPDPAGGRIERDAAGEATGMLID
                     NAMGLIAPKLPPLSFALRKEALARGTALYAARGWTHGVNMSTEADDLKALEALAAEGR
                     LPIRVDVFMTPEDAGEVLRRGPYQDATGLVRVRGVKLYMDGALGSRGAALLKPYADRP
                     DTSGLLIAEHDATLAILKQALKVRAQVAIHAIGDRGNRLALDWIAEAFASDPAAAAEV
                     RLKAPWRIEHAQILDPADIPRFAAIEVVASMQPSHAIGDLYFAPARLGPDRLAGAYAW
                     NSLYNAGALLVGGSDAPVEVGDPRIEFYAAVARKDLQGRSGPGWNPGEALSRDRALLM
                     LSSAGNAATDLWIGPPFAPGAPADITVLSRDIMTVPEADILKVTPVLTIVGGRIVHDG
                     R"
BASE COUNT          522 a         1300 c         1350 g          538 t
ORIGIN      
        1 tttgcgatag agcgtggagc ggccaatgcc cagccggcgc gcgacctccg gccccacggc
       61 ccgctgggcc gcgccgcgca gggccttctc ctcgagcgag ctgccggccc tgggcgcctc
      121 gaaggccagg ccggtcagcc gtccgacgac atgaccctcg acggtcaccg tgccatcctc
      181 ggcgacgccc gcgagcatct ccgcccggtc gccgagctgg cgcatcagca cgctggtccg
      241 ccggtcgatg aaccgggcca tcagcttctc gtgcagggcg tcggacagcc ggtcctcaag
      301 cccgcgcgtg acgccctgcc agtgggccgg atcggccagc cagttgggcc ggttggcgac
      361 ataggccagg gtgcgcaccc ccgccagccg cgtggccagg gtgtcgatct cgccatcggt
      421 gcggtcgagg ccgcggaact ggccggccat ccagtcctcg ggcatgcgcc ggcggccggt
      481 ggtcaggttg tcgaacaggg tgcggatcag ccgcaggtgc tcgtcgctgg tctgcttgcg
      541 gaagtcgggc gtctggcaga cctcccacag ccgctccagc gccacccggt cgcgcacccg
      601 gtggacgacg tcggcgtcct cagccagccg cttgagcgcc tgttcgtcga gggcctccgc
      661 cgacagggcc aacccctcgc ggtttggcgt ggcgtccagc gaacggatca gcgacggcag
      721 gttggcgaag tcgaggcggt ggttgcgcca ctgcgccccg gcgaccggct ggaaggtgtg
      781 ctcctcgacg gcggcgatga gatcctcgtc catctccccg gcctcgccgg tcacgccgaa
      841 cgtgccgtcc ttgacgtgcc ggccggcgcg gccggcgatc tgcccgacct cctgcggatg
      901 cagccagcgg gtcttccgcc cgtcgaactt gcgcaggccc gcgaacgcga catggtcgac
      961 gtccatgttc agccccatgc cgatggcgtc ggtggccacc aggaagtcga cctcgcccga
     1021 ctggtacagc gccacctggg cattgcgggt gcggggactg agactgccca tgaccaccgc
     1081 cgcgccgccg cgctggcggc ggatcagctc ggcgatggcg tagacgttgt cggcgctgaa
     1141 cgcgaccacg gcgctgcggc gcggcaggcg cgtcagcttc ttgggtcccg tgtagcgcag
     1201 ttgcgagaac cgctcccgct cgacgatctc gacgccgggc aacagccgcc ggaccagcgg
     1261 cgccatggtc ccggcgccca gcagcatggt ctcggacttg ccgcgcgcgt gcagcaggcg
     1321 gtgggtgaac acatggccgc gctcgggatc ggcgcacagc tggatctcgt cgaccgccag
     1381 gaagtcgacc tcgcggccca gcggcatggc ctcgacggtg cagacgaaat actgcggccg
     1441 cggcgggacg atcttctcct cgcccgtgat cagcgcgacc tcgcgtggcc ccttcagccg
     1501 caccacgcgg tcatagatct cgcgcgccag caaccgcagc ggcaggccga tcatgccgct
     1561 gtggtggccc agcatccgct cgacggcgag gtgggtcttg ccggtgttgg tggggccgag
     1621 caccgccacg agccggtgcg ccgcctggcc agtggaacgg tcactcatga gagaaaggtg
     1681 ggggagtcga gggcgggcgg caagggccgg ggggagggga ggagcaggga gtagggagta
     1741 gggagtaggg agtagggagt agggagtagt cgatcttgcc cgcaggtcgc accgcgccca
     1801 aacccctgct actccctact ccctactccc tacgccctgg cccttgaacg tcggctgcct
     1861 cggatggacg tgatccctga acacgtcgaa gcctttccag gcgggctcga ccggctccac
     1921 gcccggcgcg ggggtgtagg gcgacatccc ggccagttcg agcgtgggaa tgtagcgcgg
     1981 gcagttgggg aagatggccc tggcctcgac gcgcacgatc atctgggcgc cgaccgtgct
     2041 ggccagcagc ggatcgttcc ggtcgatccg cgccgttccg ttcacccgca gccgtttggg
     2101 cgccccgtgc agggcgatga acagcaggcc gacgttcgga ttgacgacca cgttgccgag
     2161 gctcttgaac atgccgttgc cgtcgtagtc cgggaaggcc agctccgagg ggccggtgac
     2221 ccgcacgaag cccgccggtc cgcccttgaa cgagcagtcg ggcgcgccgg cggcgtccgc
     2281 cgtggcgagg aagaaataga ccgcgccctc aatgaacgcc cggtcgtcgt cgctgaattc
     2341 ggtccgcctg accacgtcga gccggtcggc catgcgtcgg ctgtcgaagg cgtcctgcag
     2401 ggcgcgatgg ccttcgtggt agaatgtgtc gccggtcatc ggaacagctc cttgcgcgtg
     2461 cgatccggga gaccggcagg ataggccctg atccgccgga cgcgaaggcc gcggctggag
     2521 gcgatcctat cgcccgtcgt ggacgatcct gccgccgacg atggtcagaa ccggcgtcac
     2581 cttgaggatg tccgcctccg gcacggtcat gatgtcccgc gaaaggacgg tgatgtcggc
     2641 cggcgcgccc ggcgcgaagg gcgggccgat ccagagatcg gtcgccgcgt ttccggccga
     2701 cgagagcatc agcagcgccc ggtcgcggct cagcgcctcg ccagggttcc accccgggcc
     2761 cgaccggccc tgcaggtcct tgcgggccac cgccgcatag aattcgatgc gcgggtcgcc
     2821 gacttcgacg ggcgcgtcgg agccgcccac cagcagggcc ccggcgttgt acaggctgtt
     2881 ccaggcatag gctccggcca gccggtcggg gccgagccgg gccggggcga aatagaggtc
     2941 gccgatggcg tgcgagggct gcatcgaggc gaccacctcg atggcggcga agcggggaat
     3001 gtccgccggg tcgagaatct gcgcgtgctc gatgcgccag ggcgccttca gccgcacctc
     3061 ggcggcggcc gccgggtcac tggcgaaggc ctcggcgatc cagtccagcg ccagccggtt
     3121 gccgcggtcg ccgatggcgt ggatggcgac ctgggcgcgg accttcagcg cctgcttcag
     3181 gatcgccagg gtcgcgtcat gttcggcgat caacaggccg gacgtgtcgg gccggtcggc
     3241 gtagggcttg agcaacgccg cgccacgcga gccgagcgcg ccgtccatat agagcttcac
     3301 cccgcgcacc cggaccaggc cggtggcgtc ctggtagggg ccgcgccgca gcacctcgcc
     3361 ggcgtcctcg ggcgtcatga acacatcgac gcggatcggc aggcgaccct ccgcggccag
     3421 cgcctcgagc gccttcaggt cgtccgcctc ggtcgacatg ttgaccccgt gggtccagcc
     3481 ccgggcggcg tagagcgccg tgccccgggc cagggcctcc ttgcgcaggg cgaacgacag
     3541 cggcggcagc ttcggggcga tcaggcccat ggcgttgtcg atcagcatgc cggtggcctc
     3601 gccggcggcg tcgcgctcga tccggccgcc cgccgggtcg ggggtgtcgc gggtgatacc
     3661 cgccagtttc agggcggccg tgttcagcac caccgcgtgg ccgtcggccc
//
LOCUS       DAORNX010000312         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18738_length_3705_cov_6.406849, whole genome shotgun sequence.
ACCESSION   DAORNX010000312 DAORNX010000000
VERSION     DAORNX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18738_length_3705_cov_6.406849"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLO65_12540"
     CDS             <1..554
                     /locus_tag="PLO65_12540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29121.1"
                     /translation="MGRKAVECNMLFIEDLQVPAEDLIGEEGKGFQYLISGLNPERVL
                     FGVEAVGLGRAALARAATYAKERVVFGRPIGQNQGVQHPLAKSWAELEAANLMAFKAA
                     ALYDAGRDCGAEANAGKYLGAEAGFRACEAAVLAHGGMGYAKEYDVERYFREAMIARI
                     APVSREMILNFVAERVLGLPKSY"
     gene            complement(640..1359)
                     /locus_tag="PLO65_12545"
     CDS             complement(640..1359)
                     /locus_tag="PLO65_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRD29122.1"
                     /translation="MVAQQLLPLPAGSGRRRRLSTGTAVAVGLSVAVHAGIAAYLALA
                     AFDVIQTPFDPAPDITGGFIEVQPDRPKPLADPVKPVESQPQTAINIHDPADAVPAGV
                     ETLAAEPSSGAETAVGPMTTLDPGPITITPEAVDVPVTPMVSHPEWVRKPTAAQMERV
                     FPDRAIRLGVPGKATLACIVAANGSVGGCQVVSEDPSDFGFGKAALRLQPYFRMKPQT
                     VGGKPVDGAVVKIPVRFDLPA"
     gene            1543..>2115
                     /locus_tag="PLO65_12550"
     CDS             1543..>2115
                     /locus_tag="PLO65_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRD29123.1"
                     /translation="MAVQGIRGVVEAAIQRASQATGVDFSFLMRTASRESGYNPSAKA
                     PTSSAAGLFQFVEQTWLSTLKKHGAKYGYARYADLISLGADGRYRVSGDDARKAVMNL
                     RFDPHAASLMAGELATDHAAYLAGRIGRQPTAGELYAAHFLGPQGSARLITATRASPG
                     ASAAALFPDAAAANRPVFYRDGRALTVAEVY"
     assembly_gap    2116..2284
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2354..3547)
                     /locus_tag="PLO65_12555"
     CDS             complement(2354..3547)
                     /locus_tag="PLO65_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD29124.1"
                     /translation="MLEGLRVVELSTYIAAPGAAGIMADWGADVIKIEPPAGDPMRRF
                     FDGMAGEEHGNPVFELDNRGKRGVVLDISTPQGREAALRLTATADIFLTNVRPGALKR
                     AGLDFDSLHAAHPALIYCSLTGYGLQGADADRAGMDVAAFWSRAGVGAITAPKGVDPF
                     PLRTAQGDHITSLSTVAAILAAVHERGRTGVGRLVETSLLRTGVYSIGSDMAIQLRFG
                     KLASTRPRKQAVQPLANFFKTSDGRWICLLVRTGGTDWPQIAAAVGRPDLVENPLFAT
                     SRLRRENAADLVDILDAAFGAQTYDQVAGRLDEFDITWAPYQTPGELTRDPQAEAAGC
                     FVNTPDGKGGSFRSPASPARFSGYDDTPRSGPPALGQHTDAVLAELGYSADEIAALRA
                     GKAVA"
BASE COUNT          517 a         1205 c         1294 g          520 t
ORIGIN      
        1 agatgggccg caaggccgtc gaatgtaaca tgctcttcat cgaggacctg caggttcccg
       61 ccgaggacct gatcggcgag gagggcaagg gcttccagta cctgatcagc ggcctcaacc
      121 ccgagcgggt gctgtttggc gtcgaggccg tgggcctggg ccgcgcggcc ctggccaggg
      181 ccgcgaccta cgcgaaggag cgcgtcgtgt tcggccggcc gatcggtcag aaccagggcg
      241 ttcagcatcc gctggccaag agctgggccg aactggaggc cgccaacctg atggccttca
      301 aggcagccgc cctctacgac gccggccgcg actgcggggc cgaggccaac gccggcaagt
      361 atctcggcgc cgaggcgggc ttccgcgcct gcgaggcggc ggtgctggcc cacggcggca
      421 tgggctatgc gaaggaatac gacgtcgagc gctacttccg cgaagccatg atcgcccgca
      481 tcgctcctgt cagccgcgag atgatcctca acttcgtcgc tgaaagggtg ctggggctgc
      541 cgaagagtta ctgagccggg gacacgtacc gccttcggta cgtgtccccg tcagttgaag
      601 cggcagggga acgtactgaa gtacatgtcc ccgttttcgt tacgccggca ggtcgaaccg
      661 gacggggatc ttgaccaccg cgccgtcgac cggcttgccg ccgaccgtct gcggcttcat
      721 ccggaagtaa ggctgcagcc gcagggccgc cttgccgaag ccgaagtcgg acgggtcttc
      781 agacaccacc tggcagccgc cgaccgagcc gttggccgcc acgatgcagg ccagggtcgc
      841 cttgcccggc acgcccaggc ggatggcgcg gtcggggaat acccgttcca tctgggcggc
      901 ggtcggcttg cggacccatt cggggtggct gaccatcggg gtgaccggca cgtcgacggc
      961 ctcgggggtg atggtgatcg ggcccggatc gagcgtcgtc atcggcccca cggcggtctc
     1021 ggcgcccgac gacggctcgg cggccagggt ctcgacgccc gcggggaccg catcggccgg
     1081 atcgtggatg ttgatcgccg tctgcggctg gctctcgacc ggcttgaccg ggtcggcgag
     1141 cggcttgggc cggtcgggct ggacctcgat gaatccgccg gtgatgtccg gcgccggatc
     1201 gaagggggtc tggatgacgt cgaaggcggc cagggccaga taggcggcga tcccggcatg
     1261 gacggcgacg gacaggccga ccgcgaccgc ggtcccggtc gacaggcgcc gcctgcgccc
     1321 tgatccggcg ggaaggggga gaagctgttg agcgaccatg gcgacctccc tgggtcttgt
     1381 tgtggttgac gtagcgtcat cctgatcggc tgaaccggca atgaaattcc ggtccggccg
     1441 cgtaaaatca acgaacgttc gtttttgtgg gccagcggcg cgggggggcg gcggacgtca
     1501 tggttaatcg ccgattaacc atgacggttc aggactcgcc ccatggcggt gcagggaatc
     1561 cgtggagtcg ttgaagcggc cattcagcgc gcgtcgcagg cgacgggcgt cgatttctcg
     1621 ttcctgatgc gcaccgccag ccgcgagagc ggctacaatc cttcggccaa ggcgccgacc
     1681 tcgtccgccg ccggcctgtt ccagttcgtc gagcagacct ggctgtcgac gctgaagaag
     1741 cacggcgcca aatacggtta cgcccgctac gccgacctga tttcgctggg cgccgacggc
     1801 cgctaccggg tcagcggcga tgacgcccgc aaggcggtga tgaacctgcg cttcgacccg
     1861 cacgccgcct cgctgatggc cggcgagctg gccaccgacc acgccgccta cctggccggg
     1921 cggatcggcc gccagccgac ggccggcgag ctctatgcag cccacttcct cggcccccag
     1981 ggatcggcgc ggctgatcac ggcgacccgc gccagtcccg gggccagcgc cgcggccctg
     2041 ttccccgacg ccgcggccgc caatcgcccg gtcttctatc gtgacggccg ggccctgacg
     2101 gtggcggagg tgtacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnccgcag cctgggctcg tcgcagctga cggccgagat gctgcgggtc ctctcggacg
     2341 ccagcaagga ccgctaggcc acggccttgc cggcccgcag ggcggcgatc tcgtcggccg
     2401 agtagcccag ttccgccagc acggcgtccg tgtgctggcc cagcgccggc gggcccgacc
     2461 gcggcgtgtc gtcatagccg gaaaagcggg cgggcgacgc cggcgagcgg aagctgccgc
     2521 ccttgccgtc cggcgtgttg acgaagcagc cggccgcctc ggcctgcggg tcgcgcgtca
     2581 gctcgccggg ggtctggtag ggggcccagg tgatgtcgaa ctcgtccagc cggccggcga
     2641 cctggtcata ggtctgggcc ccgaaggccg cgtcgagaat gtcgaccagg tcggcggcgt
     2701 tttcccggcg caggcggctg gttgcgaaca gcgggttttc caccaggtcg ggccggccga
     2761 ccgcggcggc gatctgcggc cagtccgtgc cgccggtccg caccagcagg cagatccagc
     2821 gaccgtccga cgtcttgaag aagttggcca gcggctgcac ggcctgcttg cgcggccggg
     2881 tcgaggccag tttgccgaag cgcagctgga tcgccatgtc cgagccgatc gaatagacgc
     2941 cggtgcgcag cagcgaggtc tcgaccaacc ggcccacccc cgtccggccg cgctcgtgca
     3001 cggccgccag gatcgccgcg acggtggaca gcgaggtgat gtggtcgccc tgggccgtgc
     3061 gcagggggaa gggatcgacc cctttgggcg cggtgatcgc gccaacgccc gcgcgcgacc
     3121 agaaggccgc cacgtccatg cccgcccgat cagcgtcggc gccctgcagg ccatagccgg
     3181 tcaggctgca gtagatcagc gccggatggg cggcgtgcag ggagtcgaag tcgagcccgg
     3241 cccgcttgag cgccccgggg cggacattgg tcaggaagat gtcggccgtg gcggtcaggc
     3301 gcagggcggc ctcgcgaccc tggggcgtgg agatgtcgag caccacgccg cgcttgccgc
     3361 ggttgtcgag ctcgaacacc gggttgccgt gctcctcgcc ggccatcccg tcgaagaagc
     3421 ggcgcatcgg gtcgccggcc ggcggctcga tcttgatcac gtcggcgccc cagtcggcca
     3481 tgatcccggc ggcccccggc gcggcgatat aggtcgacag ttcgacgact ctcaggcctt
     3541 ccagcaagct ccgccctccc ggcttgtcat gatgtttcgg ccatcatgaa gggcggggcg
     3601 gggcggcgga aggggtcacg ccacggaacg caccgcccgg atgtccggcc gcacccgggc
     3661 ggcggcgtcg gcggcgcaag cggtcgcaac gccgcgcgcc aggtg
//
LOCUS       DAORNX010000313         3688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18854_length_3688_cov_6.245252, whole genome shotgun sequence.
ACCESSION   DAORNX010000313 DAORNX010000000
VERSION     DAORNX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18854_length_3688_cov_6.245252"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLO65_12560"
     CDS             complement(<1..684)
                     /locus_tag="PLO65_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29125.1"
                     /translation="MTDLPILHHFDSSPFSEKVRLMFGLKGLAWKSVTIPNMMPKPDY
                     TPLTGGYRRTPSLQVGADVYCDTQIILAEIERRWPEPRAVRGGLDWAINLWADRPFFQ
                     ATVPIIFGALGERTPEAFLKDREQLSGRPFDVKAMKAAMGPMSQQWRGQAAWIEDQLA
                     GSASGWLAGDGPGLADIAAWMNVWFLGGALPDTLKALTVGMPRLAGWSGRVAALGHGR
                     PEPMTGSEAL"
     gene            808..1434
                     /locus_tag="PLO65_12565"
     CDS             808..1434
                     /locus_tag="PLO65_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29126.1"
                     /translation="MTEEKIQTLKDDIAFMRALAQEGSSAPLLGGAILVAAGVTFAVA
                     GAIHWSVMSGLVNLPKATVGLVWPAALLVFLGLLLWLRRGGDNRPGAQSPGNRAASAA
                     WASMGWSIFVISLAIAILSARTHSYIPTLIFPPLILGLYGMGWSVAAAMSRKRWIWMT
                     AIGSYAGALLVAWLAIDPVVYLVYSAALLLLMALPGYVLMRQEPSDTI"
     gene            1448..1750
                     /locus_tag="PLO65_12570"
     CDS             1448..1750
                     /locus_tag="PLO65_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRD29127.1"
                     /translation="MTDGFDIDRIDEVIHGRIRLGIMAFLSGAGSADFNTLKTRLQAT
                     DGNLSAHLRKLEDAGYVAVAKTFVDRKPLTTLSLTDAGRKAFTAYLDAIGKLVEGR"
     gene            complement(1927..2403)
                     /locus_tag="PLO65_12575"
     CDS             complement(1927..2403)
                     /locus_tag="PLO65_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRD29128.1"
                     /translation="MKKILTAAIALSVLASAGAASAQSYRGRPAGYQQGYDAPRGDRR
                     DERRDERRDDRGDRRDERRDDRYDRRYENRYERRAERRYNAGRYHAPRGYQARHWRQG
                     EHLPPQYRGQAYRVDYRYYRLPPPPRGYQYTRVNDDVVLTAIATGLIASVVFNMFQ"
     gene            complement(2505..2879)
                     /locus_tag="PLO65_12580"
     CDS             complement(2505..2879)
                     /locus_tag="PLO65_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HRD29129.1"
                     /translation="MAGQTLTLRDTVVFLVAGDGPLVVGDRETSDLVGEALGAGATMI
                     AIPLPRLGPDFLRLSTRVAGELLQKLINYRFQVAILGDIAEAVAASDALRDFVRESNR
                     GRMVWFLPDLAALEAKLAMNGR"
     gene            complement(2886..3530)
                     /locus_tag="PLO65_12585"
     CDS             complement(2886..3530)
                     /locus_tag="PLO65_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29130.1"
                     /translation="MAREPVIRKGVPKGDKRQRTRRTLVAAAAEVIAERGFDRTSIEA
                     VCRRAGLSRGSFYGNFASREDLFLAVMDSQWTPIEADFRPGPPLREQMRILGEAVAAA
                     SRARLPMAAGAAAFQLYNLTHDHMRRRLTERNAETYRAMAEGFARIAPPGSLPMPTDR
                     FARVVDGLITGLVFTRFQTPDLISDEDIVAAFEALAGPAPTPGPWPMNRPITRG"
BASE COUNT          546 a         1233 c         1337 g          572 t
ORIGIN      
        1 cagcgcctcg gacccggtca tgggctccgg ccggccgtga cccagggccg ccacccggcc
       61 gctccagccg gcgagccgcg gcatgcccac ggtaagggcc ttcagggtgt cgggcagggc
      121 gccgccgagg aaccagacgt tcatccaggc ggcgatgtcg gccaggccgg ggccgtcgcc
      181 ggccaaccag cccgaggcgg acccggccag ctggtcctcg atccaggcgg cctggccgcg
      241 ccactgctgc gacatcgggc ccatggcggc cttcatcgcc ttgacgtcga acggccgtcc
      301 cgacagctgt tcgcggtcct tcaggaacgc ctccggcgtg cgctcgccca gggcgccgaa
      361 gatgatcggc accgtggcct ggaagaatgg ccggtcggcc cacaggttga tggcccagtc
      421 gagcccgccc cgcacggcgc gaggctccgg ccagcgacgc tcgatctcgg ccaggatgat
      481 ctgggtgtcg caatagacgt ccgccccgac ctgcagcgag ggcgtgcgcc ggtagccgcc
      541 ggtcagcggc gtgtagtcgg gcttgggcat catgttggga atggtcaccg acttccaggc
      601 caggcccttg aggccgaaca tcagccggac cttctcggag aagggcgagc tgtcgaagtg
      661 gtgcaggatg ggcaggtcgg tcatggcggt ccccgtttcg ggcatcggac gcgcctgcct
      721 taccggcagt ccatgaaaaa gcactttacc ttgcaaagtg caggcgccat ctacggaatc
      781 accgacgata ggccgggaga ggctgtgatg accgaagaga agatccagac cctgaaggac
      841 gacatcgcct tcatgcgcgc cctggcgcag gagggcagtt ccgcgccgct gctgggcggg
      901 gcgatcctgg tcgccgcggg cgtgaccttc gcggtcgccg gggcgattca ctggtcggtg
      961 atgtcgggcc tggtgaatct gccgaaggcg acggtcggcc tggtctggcc ggcggcgttg
     1021 ctggtgttcc tcgggctgct gctgtggctg cgccggggcg gcgacaaccg gccgggagcg
     1081 cagtcgcccg gaaaccgggc cgcgagcgcg gcctgggcgt cgatgggctg gagtatcttc
     1141 gtcatcagtc tggccatcgc catcctgtcg gcgcggaccc acagctacat cccgaccctg
     1201 atcttcccgc cgctgatcct gggcctctac ggtatgggct ggagcgtggc ggcggcgatg
     1261 tccaggaagc gctggatctg gatgacggcg atcggatcct atgccggagc cctgctggtc
     1321 gcctggctgg cgatcgatcc ggtcgtctat ctggtctatt cggccgccct gctgctgctg
     1381 atggcgctgc cgggctatgt gctgatgcgg caggaaccct cggacacgat ttgagaaagc
     1441 ccttgtcatg accgacgggt tcgacatcga ccggatcgac gaggtcatcc acggccgtat
     1501 ccgcctggga atcatggcct ttctttcggg cgccggctcg gccgacttca acacgctcaa
     1561 gacccgcctg caggccaccg acggcaacct ctcggcccac ctgcgaaagc tcgaggacgc
     1621 cggctacgtg gcggtcgcca agaccttcgt cgaccgcaag cccctgacca ccctgagcct
     1681 taccgacgcc ggccgaaagg cgttcaccgc ctacctcgac gcgatcggca agctggttga
     1741 ggggcggtag gacccgcgga cgccaggggc gagcaacacg cgcatctgcg aaaggctgga
     1801 caattgcggc gtgggtcaca cgccggatgt gtcgtgtccc ggaactgcat ccgtctcccc
     1861 aacgaaaggc gcccccggag atcgttctcc gggggcgccg tccggtcggc cttcgggggg
     1921 aagggcctac tggaacatgt tgaacacgac cgaggcgatc aggccggtgg cgatggccgt
     1981 caggacgacg tcgtcattga cccgcgtgta ctggtagccg cgcgggggcg ggggaaggcg
     2041 gtagtagcga tagtcgacgc gataggcctg gccgcgatac tgcggcggca ggtgttcgcc
     2101 ctggcgccag tggcgggcct ggtagccgcg cggcgcgtgg tagcggccgg cgttgtagcg
     2161 gcgctcggcg cggcgctcgt agcggttctc gtagcgccga tcatagcggt cgtcccggcg
     2221 ctcgtcccgg cggtcgcccc ggtcgtcacg gcgttcatcg cgccgttcgt cgcggcgatc
     2281 gccccggggg gcgtcgtagc cctgctgata gccggccgga cggccccggt aggactgggc
     2341 gctggcggcg ccggccgagg ccaggaccga cagggcgatc gcggcggtga gaatcttctt
     2401 catggctcgt ctcctggacg gccggacctc gaggcgatcc cgtggcggcc ggcgttggag
     2461 cccttatggg tgagcccgtt tgagcctcac ctgaacggcg cgggtcatct cccgttcatc
     2521 gccagcttcg cctcgagggc ggcgaggtcg ggcaggaacc agaccatgcg gccccggttc
     2581 gactcgcgca cgaagtcgcg cagggcgtcg ctggcggcca ccgcctccgc gatgtccccg
     2641 agaatcgcca cctggaagcg gtagttgatc agtttctgca acagctcccc ggccacgcgg
     2701 gtgctcaggc gcaggaagtc gggcccgagc cggggaaggg gaatcgcgat catcgtcgcg
     2761 ccggccccca aagcctcgcc gaccagatcc gaggtctccc ggtcgccgac gacgagcggg
     2821 ccgtccccgg cgacgaggaa caccaccgta tcgcgcagcg tgagggtctg accggccatg
     2881 ggacgtcagc ctcgcgtgat cggccggttc atcggccagg gccccggggt cggcgccgga
     2941 ccggccaggg cctcgaaggc cgcgacgata tcctcgtcgc tgatcaggtc cggcgtctgg
     3001 aatcgggtga acaccaggcc ggtgatcaag ccgtcgacca cccgggcgaa gcggtcggtc
     3061 ggcatcggca gggagcccgg cggggcgatg cgggcgaagc cctcggccat cgcccggtag
     3121 gtctcggcgt ttcgctcggt gagccggcgg cgcatgtggt catgggtcag attgtaaagc
     3181 tggaaggccg cggcgccggc cgccatgggc aggcgcgccc gggacgcggc ggccacggcc
     3241 tcgccgagga tgcgcatctg ctcccgaagc ggcgggcccg gccggaagtc agcctcgatc
     3301 ggcgtccact ggctgtccat gacggccagg aacaggtctt cccgactggc gaagttgccg
     3361 tagaacgagc cgcgcgacag gccggcccgg cgacagacgg cctcgatcga ggtgcggtcg
     3421 aagcctcgtt ccgcgatcac ctccgccgcg gcggcgacca gcgtcctgcg cgttcgctgg
     3481 cgcttgtcgc ccttgggaac gcccttgcgg atgaccggtt ctcgcgccat gcgcgatatc
     3541 tacggcgcca gattaaatgc aacaatgcat attttataga tgggggcgcg gcgggagctt
     3601 tcgcgcccgg ccggcgcggg cgatgctcaa cgtcgcggtt cagccggcca gggcggcggc
     3661 cagggcgatc atcgcccggc gggtgtga
//
LOCUS       DAORNX010000314         3674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18954_length_3674_cov_7.336281, whole genome shotgun sequence.
ACCESSION   DAORNX010000314 DAORNX010000000
VERSION     DAORNX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18954_length_3674_cov_7.336281"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..3073
                     /gene="topA"
                     /locus_tag="PLO65_12590"
     CDS             398..3073
                     /gene="topA"
                     /locus_tag="PLO65_12590"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRD29131.1"
                     /translation="MHVVVVESPAKAKTINKYLGSDYKVLASYGHVRDLPSKDGSVKP
                     DDDFAMSWEVDAKSAKRLSDIADAVKGADTLFLATDPDREGEAISWHLLEVLSKKKAL
                     KDVAVRRATFNAITKTAVLEAIANPREIDMELVEAYLARRALDYLVGFTLSPVLWRKL
                     PGSRSAGRVQSVALRLIVDRELEIERFRTQEYWTVEADVSAGSDPFLARLVKHEGRKL
                     SKFDLANEASASAARAAVQACSFRVASVEKKPARRTPPPPFTTSTLQQEASRKLGFSA
                     QRTMQAAQKLYEGVDIGGETTGLITYMRTDGVQSAPEALDEARRVIAGLYGKDYVPET
                     ARFYKTKAKNTQEAHEAIRPTSLERNPGKLKLESDMGRLYELIWKRMIASQMEAARIE
                     RTAVELEGSDGKTALRATGQVVQFPGYLAVYEEGRDDPDSSKSADTAGEDADYAGKLP
                     VITEGAEARVIAARADQHFTEPPPRFSEASLVKRMEELGIGRPSTYASILTVLRDRAY
                     VRMDKNRFIPEDKGRLVTAFLEEFFKRYVEYDFTAALEEKLDLVSDGKLDWKQFLRDF
                     WQDFHAAVGEIAELRVTNVLDALNLALGPHIFPAKADGSDPRLCPTCGVGQLSLKTGK
                     FGAFIGCSNYPECRFTRPIAQPDGEGDAAEGDRELGINPKTGMAVWLKNGRFGHYVEE
                     TPAEGDKPKRSSLPKGWPPAAMDIDKALRLLELPREVGLHPEDGKPIVAGIGRYGPFV
                     LHDGAYANLESADEVFDVGLNRAVAVLAEKRAGGKGGRATAAALKELGAHPEDGQAIK
                     ILNGRFGPYIKHGSTNANVPRGKEPTEITLEEAVTLLAERVAKGGGKKPVKKAAVRKA
                     VAPKKPDPLTAKTVKARKKAPAKKKPAAKAKASAD"
     gene            3346..>3674
                     /locus_tag="PLO65_12595"
     CDS             3346..>3674
                     /locus_tag="PLO65_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRD29132.1"
                     /translation="MAKKPPTRTDNSKPRTPRPPPGLPDRDTLIKYLRTAGEQDKGDI
                     ARAFGLKGEGRRALRDMLRKLEDEGALGKRGRKGFAEAGALPPVGVADVVERDPDGDL
                     YVRLVKAG"
BASE COUNT          649 a         1331 c         1228 g          466 t
ORIGIN      
        1 aggggcgtcg gcgaaccgcc cgccaggccg cgcgccgagc cgggaccgac accgacccgg
       61 aacggggttc cgacccggcg gccccaggcc gtgggcggcg cataggggtt gtccggacca
      121 ggcgggcgag acggtgctga catgctgacc ttccgcgctg ggggcggcgg cgtatgacgc
      181 cgaacgggcg atcgcggaac ccggcccggc gatccgcgaa cggtcgcacc ctccgccccg
      241 actatgccgg gagtatgacc ggcaggcggc gagatcgggc ccgcggggcc aggattcctt
      301 tgacttccac gccctgcgtt gacattgccc ccgccccgcc cccactttcc gccctcgctc
      361 gaagggcgac ccctccctca cctgtcccag agacttcatg cacgtcgtcg tcgtcgagag
      421 cccggccaag gccaagacca tcaacaagta cctcggctcg gactacaagg ttctcgcctc
      481 gtacggccac gtcagggacc tgccgtcgaa ggacggttcg gtgaagcccg acgacgactt
      541 cgcgatgagc tgggaggtcg acgccaagtc ggccaagcgc ctctccgaca tcgccgacgc
      601 cgtgaagggc gccgacaccc tgttcctcgc caccgacccc gaccgcgagg gtgaggcgat
      661 cagctggcac ctgctcgagg tgctcagcaa gaagaaggcg ctcaaggacg tcgccgtccg
      721 ccgggccacc ttcaacgcca tcaccaagac cgccgtgctg gaggccatcg ccaacccgcg
      781 cgagatcgac atggagctgg tcgaggccta cctggcccgc cgcgccctcg actatctggt
      841 cggcttcacc ctctcgcccg tgctgtggcg caagctgccg ggctcgcgct cggccggccg
      901 ggtgcagtcg gtcgccctgc ggctgatcgt cgaccgcgag cttgagatcg agcgtttccg
      961 gacccaggaa tactggaccg tcgaggccga tgtctcggcc gggtccgacc cgttcctcgc
     1021 ccggctggtc aagcacgagg gcaggaagct ctccaagttc gacctggcca acgaggcctc
     1081 ggccagcgcc gcccgcgcgg ccgtgcaggc ctgcagcttc agggtcgcct cggtcgagaa
     1141 gaagccggcc cggcgcacgc cgccgccgcc gttcaccacc tcgaccctgc agcaggaagc
     1201 ctcgcgcaag ctcggcttct cggcccagcg gaccatgcag gcggcgcaga agctgtacga
     1261 gggcgtggac atcggcggcg agaccaccgg cctgatcacc tacatgcgga cggacggcgt
     1321 gcaatcggcg cccgaagcgc tcgacgaagc ccgccgggtc atcgccggcc tttacggcaa
     1381 ggactacgtc cccgagacgg cccgcttcta caagaccaag gccaagaaca cccaggaggc
     1441 ccacgaggcg atccgcccca cctcgctgga gcgcaacccg ggcaagctga agctcgagag
     1501 cgacatgggc cggctctatg agctgatctg gaagcggatg atcgcctcgc agatggaggc
     1561 ggcgcgcatc gagcgcacgg ccgtcgagct ggaaggcagc gacggcaaga ccgccctgcg
     1621 cgcgaccggc caggtcgtgc agttccccgg ctacctcgcc gtctacgagg aaggccggga
     1681 cgacccggac tccagcaagt ccgccgacac cgccggcgag gacgccgact acgccggcaa
     1741 gctgccggtg atcaccgagg gcgccgaggc ccgggtcatc gccgcccgcg ccgaccagca
     1801 tttcaccgag ccgccgccgc gcttctccga agccagcctc gtcaagcgga tggaggagct
     1861 gggcatcggc cggccctcga cctacgcctc gatcctgact gtgctgcgcg accgcgccta
     1921 tgtgcggatg gacaagaacc gcttcattcc cgaggacaag ggccggctgg tcacggcctt
     1981 cctcgaggag ttcttcaagc gctacgtgga atatgatttc acggcggcgc tggaggagaa
     2041 gctcgacctc gtcagcgacg gcaagctcga ctggaagcag ttcctccgcg acttctggca
     2101 ggacttccac gcggccgtcg gcgagatcgc cgagctgcgc gtgaccaacg tgctggacgc
     2161 gctgaacctg gccctcggcc cgcatatctt cccggccaag gccgacggct ccgatccgcg
     2221 cctgtgcccg acctgcggcg tcggccagct gagcctgaag accggcaagt tcggcgcctt
     2281 catcggctgc tccaactatc ccgaatgccg cttcacccgc ccgatcgcgc agccggacgg
     2341 cgagggcgac gcggcagagg gcgaccggga actggggatc aatccgaaga ccggcatggc
     2401 cgtctggctg aagaacggtc gcttcggcca ctacgtcgag gagacgccgg ccgagggcga
     2461 caagcccaaa cgctccagcc tgccaaaggg ctggccgccg gcggcaatgg acatcgacaa
     2521 ggcgctgcgg ctgctggagc tgccgcgcga ggtcgggctg catcctgagg acggcaagcc
     2581 gatcgtcgcc ggcatcggcc gctacggccc gttcgtgctg cacgatggcg cctacgccaa
     2641 cctcgagagc gccgacgagg tgttcgacgt cggcctcaac cgcgccgtgg cggtgctcgc
     2701 cgagaagcgc gccggcggca agggcggacg ggccacggcc gcggcgctca aggagctggg
     2761 cgctcacccc gaggacggcc aggcgatcaa gatcctcaac ggccgcttcg gtccgtacat
     2821 caagcacggc tcgaccaacg ccaacgtccc gcgcggcaag gagccgaccg agatcacgct
     2881 tgaggaggcc gtgaccctgc tggccgaacg ggtcgccaag ggcggcggca agaagccggt
     2941 gaagaaggct gcggtcagga aggcggtcgc accgaagaag cctgatccac tgacggccaa
     3001 gacggtgaag gccaggaaga aggcgccggc gaagaagaag ccggccgcga aggccaaggc
     3061 gtcggcggac tagatcctgt cggggacatg cgcgtcactg cgtgtccccg aagccgtgcc
     3121 ctcgcggcca ggatctggtt ttccgcgcga cagcccccat atccacgcca ccgggctcac
     3181 gccgagccct tcgacggacc agcggccatg cccttgcgac aagggccctc gccgccgctt
     3241 tcgcgcccaa ccgcgaaccg ccgtcgcctc cccaacagac tcaggcgttg atgctcgtcg
     3301 gaacagtccg gcagcgtccg ccgcattgtg atattcgaaa gccacatggc caaaaaaccc
     3361 cctaccagga ccgacaacag caaaccccga acgccgaggc cgccgcccgg cctgccggac
     3421 cgtgacaccc tgatcaaata cctgcgcacc gccggggaac aggacaaggg cgacatcgcc
     3481 cgcgccttcg ggctcaaggg cgagggccgc cgggccctgc gcgacatgct gcgcaagctg
     3541 gaagacgaag gcgccctggg caagcgcggc cgcaagggct tcgccgaggc cggcgccctg
     3601 cccccggtcg gcgtcgccga cgtggtcgag cgcgatccgg acggcgatct ctacgtgcgc
     3661 ctggtcaagg ccgg
//
LOCUS       DAORNX010000315         3674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18955_length_3674_cov_7.040066, whole genome shotgun sequence.
ACCESSION   DAORNX010000315 DAORNX010000000
VERSION     DAORNX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18955_length_3674_cov_7.040066"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLO65_12600"
     CDS             <1..337
                     /locus_tag="PLO65_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRD29133.1"
                     /translation="HPERALFFRADHFSVAKRGVPVLLLMGIGGGPDLVEGGREAGDR
                     WVTDYTTRCYHQPCDNWSPAWDLRGAAADVDLLLYAGGELANNRAWPDWNEGSEFKAV
                     RAKTAGERN"
     gene            complement(429..1280)
                     /locus_tag="PLO65_12605"
     CDS             complement(429..1280)
                     /locus_tag="PLO65_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD29134.1"
                     /translation="MAYEAITTDLADGVLTITLNRPDALNAYTARMGAELADAFVEAD
                     ADDAVRVVIVTGAGRGFCAGADMSAGADAFGGDGAKLFGEGRPRTGAPKGGFVEAIYN
                     CRKPSIAAINGGAVGVGITMTLPMDIRIASTKAKIGFVFARRGLAPEAGAASFLPRIV
                     GLPQALRWCYSGKVFGPDEALAGGLVSEVVEPEALLDRAREIAREIVEETSAISIAVT
                     RALFQRFAEEPDPLKVLAVDGPLAMAMGASADVREGVSAFLEKRKPAFPGKVSEDMPA
                     PYPWWEG"
     gene            complement(1363..1542)
                     /locus_tag="PLO65_12610"
     CDS             complement(1363..1542)
                     /locus_tag="PLO65_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29135.1"
                     /translation="MSKRELIDTGTDKRYVRRDEHGRFKESDDVGKSLAADVRQKART
                     TTRPGQGDRGDQKRH"
     gene            1692..2111
                     /locus_tag="PLO65_12615"
     CDS             1692..2111
                     /locus_tag="PLO65_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29136.1"
                     /translation="MSFRERRAWISLIATPLIWGAYFWKIWPDFQDGKLHVTTFGMFI
                     GTLVLLVIVQIVLAIVMAIVAGKTVETPMDERERLIDLKGAQVGFHMLNLLLMAVASL
                     WLYGMSPLVMANGILAAMVVAETLRAASVVVGYRRGV"
     gene            2113..2328
                     /locus_tag="PLO65_12620"
     CDS             2113..2328
                     /locus_tag="PLO65_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD29137.1"
                     /translation="MAARPPIRNQIRRLRFDHGEMTQQALADAIGMTRQTIVAIEQGK
                     YSPSLEAAFRISRVFGIGVEEVFQWEE"
     gene            2377..2610
                     /locus_tag="PLO65_12625"
     CDS             2377..2610
                     /locus_tag="PLO65_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD29138.1"
                     /translation="MNTQRRVSLFRNGRNQAVRIPRDLELPGDEAVLRKEGDRLIIEP
                     VARTSLMAFLATLEPIEDDFDFGEDQPPRPVDL"
     gene            2607..3008
                     /locus_tag="PLO65_12630"
     CDS             2607..3008
                     /locus_tag="PLO65_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD29139.1"
                     /translation="MIRYLLDTNILSDLLRNPLGAVRQRVALLNEDEIGSSIIVAAEL
                     RYGAARRGSPRLTRAVEGMLQRMTILSFDRPADEIYGELRASLEASGTPVGSHDCLIA
                     AHALAVGCILVTANERDFTRVAGLKVENWLR"
     gene            complement(3013..3537)
                     /locus_tag="PLO65_12635"
     CDS             complement(3013..3537)
                     /locus_tag="PLO65_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRD29140.1"
                     /translation="MSDQPAVLISEAEVAERVAAVARRIAPRIDDETVMVCLLTGGIW
                     FAADLTRALARLGRNVHFDALWLSSYRDERHSYGRCEVRADLQRPVLGRKALIVDDVF
                     DTGLSLSEAARLVKDAGAAEVLTAVFASKPWPTERALKVDFIAWEAPARFLVGYGMDV
                     AGSMRGLPYIGAMD"
BASE COUNT          568 a         1249 c         1260 g          597 t
ORIGIN      
        1 ccatcccgaa cgggcgctgt tcttccgcgc cgaccacttc agcgtcgcca agcgcggcgt
       61 gccggtgctg ctgctgatgg gcatcggcgg cgggccggac ctggtcgagg gcggccgcga
      121 ggccggcgac cgctgggtca ccgactacac cacccgctgc taccaccagc cctgcgacaa
      181 ctggagcccg gcctgggacc tgcggggggc cgcggccgac gtcgacctgc tgctgtacgc
      241 cggcggcgag ctggccaaca accgcgcctg gccggactgg aacgagggct cggagttcaa
      301 ggccgtgcgg gcgaagacgg ccggggagcg gaactgaggg agtggggagt agggagtagg
      361 gaggagttcg gccggctcac gccgccgacg ccgcgcccac cctccagcta ctcgctactc
      421 cctactccct acccctccca ccacggatat ggcgcgggca tgtcctccga caccttgccc
      481 ggaaaggccg gcttgcgctt ctccagaaag gccgagacgc cctcgcgcac atcggcggac
      541 gcgcccatgg ccatggccag cgggccgtcg acggctagca ccttgagcgg atcgggctcc
      601 tcggcgaagc gctggaacag ggcgcgggtg accgcaatcg agatggccga tgtctcctcg
      661 acgatctcgc gggcgatctc gcgggcgcgg tcgagcaggg cttcgggctc gaccacctcg
      721 ctgaccaggc cgccggccag cgcctcgtcc gggccgaaca ccttgcccga gtagcaccag
      781 cgcagggcct gcggcaggcc gacgatgcgc ggcaggaagg aggcggcccc ggcttcgggg
      841 gccaggcccc gccgcgcgaa gacgaagccg atcttcgcct tcgtcgaggc gatgcggatg
      901 tccatcggca gggtcatggt gatgcccacc ccgaccgccc cgccgttgat cgccgcgatc
      961 gacggcttgc ggcagttgta gatcgcctcg acgaagccgc ctttcggcgc gcccgtgcgg
     1021 ggccggccct cgccgaacag cttcgcgcca tcgccgccga aggcgtccgc ccccgccgac
     1081 atgtccgctc cggcgcagaa gccccgcccc gcgccggtga cgatgaccac ccgcaccgca
     1141 tcgtccgcgt cagcctcgac gaaggcgtcg gccagttcgg cgcccatccg cgccgtatag
     1201 gcgttgagcg cgtcggggcg gttcagggtg atcgtcagaa cgccgtccgc gaggtcggtg
     1261 gtgatcgcct cataggccat ggccgtctcc tccgcgcccg ttgcccgggc gtcgggagca
     1321 gcctgtcagg ccgtgcgggg cggcgacaag tgtgacgtgg cgtcagtgac gcttctggtc
     1381 gccgcggtcg ccctggccgg gtctggtcgt ggtcctggcc ttctggcgca catcggcggc
     1441 cagcgacttg ccgacgtcgt cggactcctt gaaccggcca tgctcgtcgc ggcgcacgta
     1501 gcgcttgtcg gttccggtat cgatcagttc gcgtttggac atggggaaag ctcctgtttg
     1561 aagcaggaat ccctttgcgc gccgggggtt ccggtcgctg gcgtcgcagc atgaatgttt
     1621 tactggacaa tttgtccgat attttttaca caatgaccaa catacaggac actcaccggg
     1681 agattgccgc catgtccttt cgcgaacgcc gcgcctggat ctcgctgatc gccacgccgc
     1741 tcatctgggg ggcctacttc tggaagatct ggcccgactt ccaggacggc aagctgcatg
     1801 tgaccacctt cggcatgttc ataggcaccc tggtgctgct ggtgatcgtc cagatcgtgc
     1861 tggccatcgt gatggctatc gtcgcgggca agaccgttga aaccccgatg gacgagcggg
     1921 aacggttgat cgacctgaag ggcgcccagg tcggcttcca catgctgaac ctgctgctga
     1981 tggccgtggc gtcgctctgg ctgtacggca tgagcccgct ggtgatggcc aacggcatcc
     2041 tggcggccat ggtggtggcc gagaccctgc gcgcggccag cgtcgtcgtc ggctatcgcc
     2101 ggggggtctg agatggccgc ccggcccccc atccgcaacc agatccgccg cctgcgcttc
     2161 gaccatggcg agatgaccca gcaggccctg gccgacgcca tcggcatgac ccgccagacc
     2221 atcgtcgcca tcgaacaggg caagtactcg ccctcgctgg aggcggcgtt ccgcatttcg
     2281 agggtgttcg ggatcggcgt ggaggaggtg ttccagtggg aggagtgaag cgcgaggcga
     2341 gatgacatat gccgtggcat atgctagcta gacggcatga atacccagcg acgcgtcagc
     2401 ctctttcgca acggccgcaa ccaagccgtg cgcattccgc gcgatctgga actgccaggc
     2461 gacgaagcgg tcctccgcaa ggagggcgac cggctcataa tcgagcccgt ggccagaaca
     2521 tcgctgatgg ccttccttgc gacccttgag cctatcgagg atgacttcga cttcggcgag
     2581 gaccagccgc cgaggcccgt ggatctttga tccgctacct cctcgacacg aatatcctct
     2641 ccgaccttct tcggaacccg ctcggcgcgg ttcgacaacg cgtcgccctt ctgaatgagg
     2701 acgagatcgg ttccagcatc atcgtcgccg ccgagcttcg ctacggcgcc gcaaggcgag
     2761 ggtcgccgag gctgacaaga gcggtcgaag gcatgctcca gcgcatgacc atcctttcct
     2821 ttgatcgtcc cgctgacgaa atctatggcg aattgcgagc ctctctggag gcgtcgggga
     2881 cgcccgtcgg cagccacgat tgtctgatcg cagcccatgc cttggccgtg ggctgtatcc
     2941 tggttaccgc caacgaacgg gatttcaccc gggttgccgg cctgaaggtc gaaaactggc
     3001 tccgctagct gttcagtcca tcgccccgat gtagggcagg ccgcgcatgc tgccggcgac
     3061 gtccatgccg tagccgacga ggaaccgggc cggggcttcc caggcgatga agtcgacctt
     3121 gagcgcgcgt tcggtcggcc agggcttgct ggcgaagacg gcggtcagga cctcggccgc
     3181 gccggcgtcc ttgaccaggc gggcggcctc gctgagcgac aggccggtgt cgaacacgtc
     3241 gtcgacgatc agcgccttgc ggccgagcac cgggcgctgc aggtcggcgc ggacctcgca
     3301 gcggccatag ctgtggcgct cgtcgcgata ggacgacagc cacagggcgt cgaagtgtac
     3361 attgcggccc agccgggcca gggcgcgggt caggtccgcg gcgaaccaga tgcccccggt
     3421 cagcaggcag accatgaccg tctcgtcgtc gatgcgcggc gcgatccggc gagccaccgc
     3481 ggcgacccgc tcggcgacct cggcctcgga aatcaggaca gcgggctggt cactcatcag
     3541 tggccttccg ttggcagggc gtagggcgag ctttccggca gggggtgggc ctcgaccacc
     3601 gtcgcgccgg ctgcgggcgt ggcggccggc ggcgggggaa cggtcggttc ggccgcccca
     3661 cgcagctcca ggcg
//
LOCUS       DAORNX010000316         3672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_18962_length_3672_cov_5.909041, whole genome shotgun sequence.
ACCESSION   DAORNX010000316 DAORNX010000000
VERSION     DAORNX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18962_length_3672_cov_5.909041"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    227..294
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <295..528
                     /locus_tag="PLO65_12640"
     CDS             <295..528
                     /locus_tag="PLO65_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HRD29141.1"
                     /translation="GAVLVKVVDRRAGRARLYSEATRGDGERIWMQPLVSDSEADLDA
                     YAQRTLRIDPDIWIVEIDDAQGRHFLVEPVEQR"
     gene            615..995
                     /locus_tag="PLO65_12645"
     CDS             615..995
                     /locus_tag="PLO65_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29142.1"
                     /translation="MLFLMNDVVLNLDALELAPPVAARRFARLSHGFIDRLGAELFSE
                     DPLLQRRDVERAQRLAALIMAKTPEVNAALFVAPSAGCPVDHVQSRYCQIGLEVMGLL
                     YERQKHGTLTTVEADRQVWRRLAA"
     gene            1120..2580
                     /locus_tag="PLO65_12650"
     CDS             1120..2580
                     /locus_tag="PLO65_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HRD29143.1"
                     /translation="MAMAAALGLLLLAPATAQAKWLRAESARFVVYSDGDEATLRAYA
                     NKLEDFDTILRVFYGLSPNGVPAQKLHVYLVRRRASMARVWPDIPESTAGFYSSSTNG
                     IFAIAENVPGDSEADDVMLHEYTHHFMLQNLRGVFPGWLVEGFAEYYMTARLGPSSFV
                     IGNPNSGRAYQLMNERWVPTEEVLSKRPLEVADSQRGGYYAQAWLMTHYMLSDPGRRQ
                     QLFAYLRKLAAGEGSVDAWEAATGEDVATLDRKLQAYKKGNLAATGFKRKPHPAAEIT
                     VAALPPSADGLLLESLRMTRGLYDHEQKDFVAAVRAAAARYPGDRFAALTLARAEVEY
                     GDLAAGDALLDGLIAADAGDSEALVIKALGLMRAGDDDSADPRKLYGQAGKLLARANS
                     LTPDDYRILYAFAETRRTDPDYPSDNTLNVLMLAAELAPQVYGIRVEAARGLILRKRF
                     DEAIEQLTPVANSPHAGGAAEAARTLLRQIALYRGS"
     gene            2640..>3672
                     /locus_tag="PLO65_12655"
     CDS             2640..>3672
                     /locus_tag="PLO65_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29144.1"
                     /translation="MARRSGLFRGLAALVFGLLLAVGGAASQAQARWLRAETRHFVLY
                     SDGTEANLRRYAADLEDFDALLRSVHGLDTAAAPARKLDIYLVAGPRDLSRVLPGASQ
                     SVAGLYRANLGDIYAVAVRGDAGTGQGQRFLFHEYVHHFMLQNFAAAYPAWLVEGYAE
                     YFGATRIEPDRIEIGVAPPGRARNLANLPWLPLTTIAGLAPPRAQTRGAQPVMAMYYA
                     QSWLLTHYLLSDPARKARLNAYLLAVGRGEDPTAALETALGMDLSSAEKALKAYFHAT
                     PGIRYPRAGRPEVALAVTVLPPSADALLLEAQRLKSGVPAVERAGFLALIRERAARFP
                     DDGLAQLTLA"
BASE COUNT          529 a         1286 c         1237 g          552 t
ORIGIN      
        1 aacaggtcca gggctccgcc ggccacggcc gcgcccggag cggcgcagac ggccgccgcc
       61 agcagaagtc cccgccatcg cggggcggcc tccgggcgcg ggctcgtcat gggtcggtct
      121 tttcccgtat gctgtcgaaa ggctggcgca gcctaggcgc cgcgagttgc ggttcggtta
      181 gcgggaacgg tcttgcttct ttccacggat atctgggtcg gcgcccnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgcg
      301 gtgctggtca aggtcgtcga ccgccgggcc ggtcgcgccc ggctctacag cgaggcgacc
      361 cggggcgatg gcgagcggat ctggatgcag ccgctggtgt ccgacagtga ggcggatctc
      421 gacgcctacg cccagcgcac cctgcgcatc gacccggaca tctggatcgt cgagatcgac
      481 gacgcacagg gacggcattt tctcgtcgag ccggtggaac agcgttagcg gcggcgttaa
      541 ccgcttttct acaccggact gaaagccagg gcggcccata tcgggggcgt tgtttcctgt
      601 tccggcgctc cccgatgctt ttcctcatga acgacgtcgt gctgaacctc gacgccctcg
      661 agctcgcgcc gccggtcgcg gcgcgtcgtt tcgcccggct cagccacggc ttcatcgatc
      721 gcctcggggc cgaactgttt tccgaggacc cgctgttgca gcgccgcgat gtcgagcgcg
      781 cccagcggct ggccgcgctg atcatggcca agacgccgga ggtcaacgcc gccctgttcg
      841 tggcgccgtc ggccggctgt ccggtcgacc atgtccagtc gcgatactgc cagatcggcc
      901 tggaggtgat gggcctactc tatgagcggc agaaacacgg aaccctgacg accgtcgaag
      961 ccgaccggca ggtctggcgc cgactcgcgg cctgaccctg gggcgtattc ccggcccgcg
     1021 cgctctttac gcgcgcgcga cttgccgcct agactgtgcc tcagggttga ggcgcgtacg
     1081 ccagggggga cggcgatgcc gggatttcga cggtggggca tggccatggc ggctgccctg
     1141 gggctgctgc tgctggcgcc ggcgaccgcc caggccaagt ggctgcgcgc cgaaagcgcg
     1201 cggttcgtcg tctacagcga cggcgacgag gcgacgctga gggcctacgc caacaagctg
     1261 gaagacttcg acaccatcct gcgggtcttc tacggcctca gccccaacgg cgtgcctgcg
     1321 cagaagctgc atgtctatct ggtccgccgg cgcgcgagca tggcgcgcgt atggcccgac
     1381 atccccgaga gcaccgccgg cttctattcg tcctcgacca acggaatctt cgccatcgcc
     1441 gagaacgtgc ccggcgattc cgaagccgac gacgtgatgc ttcacgagta cacccaccat
     1501 ttcatgctgc agaacctgcg cggcgtgttc ccgggatggc tcgtcgaggg cttcgccgaa
     1561 tactacatga ccgcccggct cgggccgtcg agcttcgtca ttggcaaccc caacagcggc
     1621 cgggcctacc agttgatgaa cgagcggtgg gtgccgaccg aggaggtcct cagtaagcgg
     1681 ccgcttgaag tggccgacag ccagcggggt ggctactacg cccaggcctg gctgatgacc
     1741 cactacatgt tgtccgatcc cggccggcgg cagcagctgt tcgcctacct caggaaactg
     1801 gccgcgggcg aaggttcggt ggacgcctgg gaggcggcca ccggcgagga cgtggcgacc
     1861 ctcgacagga agctgcaggc ctacaagaag ggcaatctgg ccgcgaccgg attcaagcgc
     1921 aaaccgcatc cggcggccga gatcaccgtc gccgccctgc cgccgtccgc ggacggcctg
     1981 cttctggaaa gcctgcgcat gacgcgcggc ctctacgacc acgaacagaa ggatttcgtc
     2041 gccgccgtgc gcgcggccgc ggccaggtac ccgggcgacc ggttcgccgc actgaccctg
     2101 gcccgggccg aggtcgagta cggcgacctc gccgccggag acgcgttgct ggacgggctg
     2161 atcgccgccg acgccggcga ctccgaagcc ctggtgatca aggcgctggg gctgatgcgg
     2221 gccggcgacg acgattcagc cgacccccgc aaactctatg gccaggccgg aaagctgctg
     2281 gcccgggcca acagcctgac gccggacgac tatcgcatcc tctacgcctt cgccgagacg
     2341 cgccggaccg atccggacta tccgtcagac aacaccctca acgtcctgat gctggcggcc
     2401 gagctggcgc cgcaggtcta tggcatccgg gtcgaggcgg cccgcggcct gatcctgcgc
     2461 aaacggttcg acgaggccat cgaacagctg acgccggtcg ccaacagccc ccatgccggc
     2521 ggggcggcgg aggcggccag gacgctgctc aggcagatcg ctctgtaccg cggttcgtga
     2581 caaccgccgc caggaccgct accgccctct ccatgaccca gcgcccgcga cccgacgcca
     2641 tggcccgacg ctccggcctg ttccgcggct tggcggccct tgtcttcggc ctgctgctgg
     2701 ccgtcggcgg ggccgcgtcg caggcgcagg cgcgctggct gcgggccgag acccgtcact
     2761 tcgtgctcta cagcgacggc acggaggcca accttcgccg gtacgcggcc gacctcgagg
     2821 acttcgacgc cctgctccgg tccgtgcacg gcctggacac cgccgcggcg ccggcccgga
     2881 agctggatat ctacctcgtc gccgggccgc gcgacctgtc gcgggtgctt ccgggcgcct
     2941 cgcagtcggt cgcgggtctc taccgcgcca acctcggcga catctacgcc gtggccgtgc
     3001 ggggggacgc cgggaccggc cagggccagc ggttcctgtt ccacgagtat gtgcaccact
     3061 tcatgctgca gaacttcgcc gcggcctatc cggcctggtt ggtggagggc tacgccgagt
     3121 atttcggagc cacgcgcatc gagccggacc gcatcgagat cggcgtggcg ccgccgggac
     3181 gggcgcgcaa tctggccaat ctgccgtggc tgccgctgac caccatcgcg ggcctcgcgc
     3241 cgccacgggc ccagacccgg ggagcccagc cggtcatggc gatgtactac gcccagtcct
     3301 ggctgctcac ccactacctg ctcagcgatc cggcgcggaa ggcgcggctg aacgcctatc
     3361 tgctggcggt gggccgtggc gaggacccga ccgccgctct ggagaccgcg ctgggcatgg
     3421 acctttcgtc ggccgagaag gcgctcaagg cttacttcca cgccacgccg ggcattcgct
     3481 acccgcgggc cgggcgaccg gaggtcgcgc tggcggtgac ggtcctgccg ccgtccgccg
     3541 acgccctcct gctcgaggcg caacggttga agagcggcgt cccggccgtc gagcgggccg
     3601 ggtttctcgc cctgatccgc gaacgggcgg cgcgcttccc tgacgacggc ctggcgcagc
     3661 tgaccctggc ga
//
LOCUS       DAORNX010000317         3654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19075_length_3654_cov_10.346763, whole genome shotgun
            sequence.
ACCESSION   DAORNX010000317 DAORNX010000000
VERSION     DAORNX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_3654_cov_10.346763"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2759)
                     /gene="rpoC"
                     /locus_tag="PLO65_12660"
     CDS             complement(<1..2759)
                     /gene="rpoC"
                     /locus_tag="PLO65_12660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRD29145.1"
                     /translation="MNQEVLNIFNPVQAAPTFDRIRIALASPEKIRSWSFGEIKKPET
                     INYRTFKPERDGLFCARIFGPTKDYECLCGKYKRMKYKGIICEKCGVEVTLARVRRER
                     MGHIELASPVAHIWFLKSLPSRIAMMLDMPLKDIERVLYFEHYIVTEPGLTALKQHQL
                     LSEDEYMRFQDEFGDDSFTAEIGAEAVFGLLKAIDLPKEADRLREELAGTPSEMKQKK
                     ASKRLKIIEAFIESGNKPEWMILTVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLIELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKKMALELFKPFIYARLDAKGL
                     SGTVKQSKRMVEREQPAVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPKLIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILSPANGRPIIVPSQDI
                     VLGLYYLSGARDNEPGEGKLFADLGEIEAAMDAGIVTLHAKIKARFTEMDADGNLVRK
                     VIDTTPGRMKIASLLPHHPQVGHRLIEKALTKKEIGNLIDIVYRHCGQKATVIFADQM
                     MALGFKEAARAGISFGKDDIVIPERKQPIVAETRKLAEEYEQQYADGLITKGEKYNKV
                     VDAWSKATDRIADEMMLEISTKKTGDDGREKEINAIYMMAHSGARGSQAQMKQLGGMR
                     GLMAKPSGEIIETPIVSNFKEGLTVQEYFNSTHGARKGLADTALKTANSGYLTRRLVD
                     VAQDSIITEEDCGTTRGITLKAVVEGGDVLVSLGLRVLGRFSAEDIKDPATGEVLIPA
                     DTYLVEDFADLIDKAGVQSVKVRSVLTCEAGIGVCGACYGRDLARGTPVNIGEAVGVI
                     AAQSIG"
     gene            complement(2848..>3654)
                     /locus_tag="PLO65_12665"
     CDS             complement(2848..>3654)
                     /locus_tag="PLO65_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRD29146.1"
                     /translation="GNKGVISKILPIEDMPHLADGTAVDVVLNPLGVPSRMNVGQIFE
                     THLGWAAAGLGKQIANLLEAWQNGGQKKALQDRLREVYGPDEDLPDDEGELIELARNL
                     SKGVPFATPVFDGAHIPDIEDLLESAGLHRSGQSVLFDGQTGEQFRRPVTVGYIYMLK
                     LHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLT
                     VKSDDVAGRTKVYESIVRGDDTFEAGIPESFNVLVKEMRSLGLNVELENS"
BASE COUNT          544 a         1168 c         1231 g          711 t
ORIGIN      
        1 ccgatcgact gggcggcgat gacgcccacg gcttcaccga tgttcaccgg cgtgccgcgc
       61 gccaggtcgc ggccgtagca ggcgccgcag acgccgatgc cggcctcgca ggtcagcacc
      121 gagcgaacct tgacgctctg aacgccggcc ttgtcgatca ggtcggcgaa atcctcgacc
      181 agataggtgt cggccggaat cagcacctcg ccggtcgccg gatccttgat gtcctcggcg
      241 gagaagcggc ccaggacccg caggcccagc gagaccagca cgtcgccacc ctcgaccacg
      301 gccttcaggg tgatgccccg ggtggtgccg caatcttcct cggtgatgat gctgtcctgc
      361 gccacgtcga cgaggcggcg ggtcaggtag ccggagttgg ccgtcttcag cgccgtgtcg
      421 gccaggccct tgcgggcgcc gtgggtggag ttgaagtact cctgcacggt caggccttcc
      481 ttgaagttcg agacgatcgg ggtctcgatg atctcgccgg acggcttggc catcaggccg
      541 cgcattccgc cgagctgctt catctgggcc tgcgagccgc gagcgccgga gtgggccatc
      601 atgtagatgg cgttgatctc cttctcgcga ccgtcgtcgc cggtcttctt ggtcgagatc
      661 tccagcatca tctcgtcggc gatccggtcc gtggccttgg accaggcgtc gacgaccttg
      721 ttgtacttct cgcccttggt gatcaggcca tcggcgtact gctgctcgta ttcctcggcc
      781 agcttgcgcg tctcggcgac gatcggctgc ttgcgctcgg gaatgacgat gtcgtccttg
      841 ccgaaggaga tgccggcgcg ggccgcttcc ttgaagccca gggccatcat ctggtcggcg
      901 aagatgaccg tcgccttctg accgcagtgg cggtagacga tgtcgatcag attgccgatt
      961 tccttcttgg tcagcgcctt ttcgatcagg cggtgaccga cctgcgggtg atgcggcagc
     1021 agcgaggcga tcttcatccg ccccggcgtc gtgtcgatca ccttgcgcac cagattgccg
     1081 tccgcgtcca tctcggtgaa gcgggccttg atcttggcgt gcagggtcac gatgccggcg
     1141 tccatggccg cctcgatctc gcccaggtcg gcgaacagct tgccctcgcc cggctcgttg
     1201 tcgcgcgcgc cggacaggta gtagaggccc agcacgatat cctgcgacgg cacgatgatc
     1261 ggccggccgt tggcggggct gaggatgttg ttggtcgaca tcatcagcac gcgcgcttcc
     1321 agctgcgcct cgaggctcag cgggacgtgc acggccatct ggtcgccgtc gaagtcggcg
     1381 ttgaacgcgg cgcagaccag cgggtgcagc tggatggcct tgccctcgat cagcttgggc
     1441 tcgaacgcct ggatgcccag gcggtggagg gtcggcgcgc ggttcaacag taccggatgc
     1501 tcgcggatga cctcctcgag gacgtcccag acggccggct gctcgcgctc gaccatgcgc
     1561 ttggactgct tgacggtgcc cgacaggccc ttggcgtcca gccgcgcgta gatgaacggc
     1621 ttgaacagct cgagcgccat cttcttgggc aggccgcact catgcagctt cagctcggga
     1681 ccgacgacga tgaccgaacg gcccgaatag tcgacgcgct tgccgagcag gttttggcgg
     1741 aaccggccct gcttgccctt gagcatgtcg gcgagggact tcagcgggcg cttgttggcg
     1801 ccggtgatga cccggccgcg acggccgttg tcgaacaggg cgtcgacggc ttcctgcagc
     1861 atccgctttt cgttgcggat gatgatgtcg ggcgcgcgca gctcgatcag gcgcttgagg
     1921 cggttgttgc ggttgatcac ccggcggtag aggtcgttca ggtcggaggt cgcgaagcgg
     1981 ccgccgtcca ggggcaccag cgggcgcagc tcgggcggaa tgaccgggac gaccgtcagg
     2041 atcatccatt ccggcttgtt gccggactcg atgaaggcct cgatgatctt caggcgcttg
     2101 gacgccttct tctgcttcat ttccgagggg gtgccggcca gctcttcacg caggcggtcg
     2161 gcttccttgg gcaggtcgat ggccttgagc aggccgaaca ccgcctcggc gccgatctcg
     2221 gcggtgaagc tgtcgtcgcc gaactcgtcc tggaagcgca tgtactcgtc ttccgacagc
     2281 agctggtgct gcttcagcgc ggtcaggccc ggctcggtga cgatgtagtg ctcgaagtac
     2341 aggacccgct cgatgtcctt cagcggcatg tcgagcatca tggcgatgcg cgagggcagc
     2401 gacttcagga accagatgtg ggcgaccggg ctggccagtt cgatgtggcc catccgctcg
     2461 cgccggacgc gggcgagggt gacttccacg ccgcactttt cgcagatgat gcccttgtac
     2521 ttcatccgct tgtacttgcc gcacaggcac tcgtagtcct tcgtcggacc gaagatgcgc
     2581 gcgcagaaca ggccgtcacg ctcgggcttg aacgtgcggt agttgatggt ttccggcttc
     2641 ttgatctcgc cgaacgacca cgaacggatc ttctcgggcg aggccagggc gatgcggatg
     2701 cggtcgaagg tcggagcggc ctggaccgga ttgaagatgt tcaggacttc ctggttcatc
     2761 ttttttcctt ctgcgggatc ccccgcgaaa atttcagagc aagacgggcg gtcgcccctc
     2821 ccctggccgg ggaggggttc atgaccatca gctgttctcg agctcgacgt tcaggcccag
     2881 ggagcgcatt tccttgacca gcacgttgaa gctttccggg atgccggcct cgaacgtgtc
     2941 gtcgccgcgc acgatggact cgtagacctt cgtacggccg gccacgtcgt ccgacttcac
     3001 cgtcagcatt tcctgcaggg tgtaggcggc gccgtaggct tcgagcgccc acacttccat
     3061 ttccccgaag cgctggccgc cgaactgcgc cttgccgccc agcggttgct gggtgacgag
     3121 cgagtaggga ccgatcgagc gggcgtggat cttgtcgtcc accaggtggt gcagcttgag
     3181 catgtagatg tagccgaccg tgaccgggcg acggaactgc tcgccggtct ggccgtcgaa
     3241 caggaccgat tgacccgaac ggtgcaggcc cgccgactca agcaggtcct cgatgtccgg
     3301 gatgtgcgcg ccgtcgaaga ccggcgtggc gaagggcacg cccttggaca ggttgcgggc
     3361 cagctcgatc agctcgccct cgtcgtccgg caggtcctcg tccggcccgt agacttcgcg
     3421 cagacggtcc tggagggcct tcttctgccc gccgttctgc caggcctcga ggaggttggc
     3481 gatctgcttg cccagccccg ccgcggccca gccgaggtgg gtttcgaaga tctgccccac
     3541 gttcatgcgc gagggcacgc ccagcgggtt gagcacgaca tcgacggcgg tgccgtcggc
     3601 caggtgcggc atgtcctcga tcggcaggat cttggagatg acgcccttgt tgcc
//
LOCUS       DAORNX010000318         3647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19122_length_3647_cov_4.687639, whole genome shotgun sequence.
ACCESSION   DAORNX010000318 DAORNX010000000
VERSION     DAORNX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19122_length_3647_cov_4.687639"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLO65_12670"
     CDS             <1..391
                     /locus_tag="PLO65_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29147.1"
                     /translation="LAGGNVAARGNRTEGAVLGGLLGAIVGANVGRSAAACEPGTYTQ
                     GYAPAPQGGYYGYEGGPDGYQQGAYDDRYGYDDGYDGGYARPVTDAPAADNCTLAESP
                     IYLPDGRVQKRFVRVCRDSAGRYQVVD"
     gene            complement(453..1184)
                     /locus_tag="PLO65_12675"
     CDS             complement(453..1184)
                     /locus_tag="PLO65_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRD29148.1"
                     /translation="MMKRRIDLLLVERGLFDSRARAQAAIAAGRVTVDGRAVGKPSEA
                     VEPDAVITAEAAHPWVGRGGVKLDHALTLWPVAVEGRVVLDVGASTGGFTQVCLGRGA
                     ARVYAVDVGQGQLHPAVAADRRVVELSGVDARTLDASRIPQGPDLIVSDVSFISLIKV
                     LPAALALAREGADLVALVKPQFEVGRDGVGKGGVVRNPAVRQAALEQVADWLTGEGWP
                     VRATADSPIAGGDGNAEFLLWARRS"
     gene            complement(1361..2854)
                     /gene="glpK"
                     /locus_tag="PLO65_12680"
     CDS             complement(1361..2854)
                     /gene="glpK"
                     /locus_tag="PLO65_12680"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRD29149.1"
                     /translation="MSDPLILALDQGTTSTRAIAFDAHGRAVADASRPLRQSYPHDGW
                     VEHDAEEIWNASVTVLRETLGKCGRTVAEVAAIGVTNQRETVVVWDRATGRPIHPAIV
                     WQDRRTAGACERLVKAGAEERVTEVTGLLLDPYFSGTKIAWLLDNVDGARERAERGEL
                     LCGTMDSWVIWKLTGGRVHATDATNASRTLLFDIGAQRWSDEMLRLFDVPAALLPEVL
                     DCAGDYGEADAAILGRAVPIRGVAGDQQAALMGQGCVRPGEMKATYGTGCFMLLNTGE
                     QKALSRSRLLTTVAARVAGRTTYALEGSIFVAGAAIQWLNEGLGLAGGPRAAESLART
                     ANPDSGVVVVPAFTGLGAPWWDAGARGGIFGLTRDSGLAEITAATFDACALQTRDLIE
                     AMRADAPHAFGDKAELRIDGGMSQSAWFSQRLADLTGIPVCRASYQETTALGAALFAG
                     LGAGLFSTVEEAASARPDAELMTPSRDSHMREAAYARWLDAVPRVRS"
     gene            2955..>3647
                     /locus_tag="PLO65_12685"
     CDS             2955..>3647
                     /locus_tag="PLO65_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HRD29150.1"
                     /translation="MTIAVEAADLLLLARSRHPVDRERLLNAIVALCDRADIDEVAQP
                     RVRELVGSVFMALVAEAERDIRRRLAEKIAAAPWVPAALVNVLALDDIEIAGPVIAAS
                     PVLQDHDLIRLLVEATLDHQIAIARRGRLSAPVVEAILRQEEPAVLTALAGNDTAEIT
                     HSAMERLVETSRGVAAMRTPLARHPRLSSDLAQRLYLWVGQSLRASLVERFRLDPAEL
                     DAALALTVREAHA"
BASE COUNT          507 a         1366 c         1260 g          514 t
ORIGIN      
        1 cctcgccggc ggcaacgtcg ccgcgcgcgg caaccgcacc gagggcgcgg tgctgggggg
       61 cctgctcggc gccatcgtcg gggccaacgt cggccggtca gcggcggcct gcgagccggg
      121 gacctatacc cagggctacg ctcccgcgcc gcagggcggc tactacggct acgaaggcgg
      181 gcccgacggc taccagcaag gcgcctacga cgaccgctac ggctatgacg acggctatga
      241 cggcgggtac gcccggcccg tgaccgacgc cccggcggcc gacaactgca ccctcgccga
      301 aagcccgatc tacctgcccg acggccgcgt ccagaagcgc ttcgtgcgcg tctgtcgcga
      361 ctccgccgga cgctatcagg tcgtcgactg acccctgtcg acgtcctctg tgagggaagg
      421 ccgtccgcct ggcgcgggcg gccttttccc tgtcagctcc gccgcgccca gagcaggaac
      481 tcggcgttgc cgtcgccgcc ggcgatcggg ctgtcggctg tcgcgcggac gggccagccc
      541 tcgccggtca gccagtcggc cacctgctcc agagcggcct gacgcacggc ggggttcctg
      601 acgacgccgc ccttgccgac gccgtcgcga ccgacctcga actggggttt gaccagggcg
      661 acaagatccg caccctcgcg cgccagcgca agagcggccg gcaagacctt gatcaggctt
      721 atgaaactta cgtcactcac gatcaggtcg gggccttgcg gaatccgtga cgcgtcaagc
      781 gttcgggcat cgacccccga cagctcgacg acccgccgat cggccgccac cgccgggtgc
      841 agctggccct ggccgacatc gaccgcgtag acccgcgccg cgccccggcc caggcagacc
      901 tgggtgaacc cgccggtcga ggcccccacg tcaagcacga cccgaccttc cacggcgacc
      961 ggccacaggg tcagggcgtg gtccagcttg accccgcccc gtccgaccca cggatgggcg
     1021 gcctcggcgg tgatgacggc gtccggctcc accgcctcgg acggcttgcc gaccgcgcgg
     1081 ccgtcgaccg tcacccgtcc cgccgcgatc gccgcctggg cccgggcgcg gctgtcgaac
     1141 aggccccgct cgacgaggag gagatcgatg cggcgcttca tcatcgcgtg gttatggccc
     1201 ttctcccctt gcgggagaag atggccctgc ggagcagggt cggatgaggg ggcgatgcga
     1261 cgtatccttc gaaggtgatg aagcctcccg gccggaggcc gagacccctc gtccgtcggc
     1321 tacgccgaga ccttccccca caaggggaga agggttccga tcacgaacgc accctcggca
     1381 cggcgtcgag ccagcgggcg taggcggcct cacgcatgtg gctgtcgcgc gacggggtca
     1441 tcagctcggc gtcggggcgg gccgaagcgg cttcctcgac cgtcgagaac aggcccgcac
     1501 cgaggcctgc gaacagggcg gcgccgagcg ccgtggtctc ctggtagctg gcgcggcaga
     1561 ccgggatgcc ggtgaggtcg gccaggcgct gcgagaacca ggcgctctgg gacatgccgc
     1621 catcgatgcg cagctcggcc ttgtcgccga acgcatgcgg cgcgtcggcg cgcatcgcct
     1681 cgatcaggtc gcgggtctgc agggcgcagg cgtcgaaggt cgccgcggtg atctcggcca
     1741 ggccggagtc ccgtgtcagg ccgaagatgc cgccgcgcgc gccggcgtcc caccagggcg
     1801 cgccgaggcc ggtgaaggcc ggcaccacca ccacgccgct gtcgggattg gccgttctgg
     1861 ccagcgactc cgccgcgcgc gggccgcccg ccaggccgag cccttcattc agccactgga
     1921 tcgcggcccc ggcgacgaaa atcgagccct cgagggcgta ggtggtgcgg cccgctaccc
     1981 gcgccgccac cgtggtcaac aggcgcgaac ggctgagcgc cttctgctcg ccggtgttga
     2041 gcagcatgaa gcagccggtg ccgtaggtcg ccttcatctc gccgggcctg acacagccct
     2101 gccccatcag cgccgcctgc tggtcgccgg ccacgccgcg gatcggcacg gcgcggccga
     2161 ggatcgccgc atccgcttcg ccgtagtcgc cggcgcagtc gagcacctcg ggcagcaggg
     2221 cggcgggaac atcgaacagc cgcagcatct cgtccgacca gcgctgggcg ccgatgtcga
     2281 acagcagggt gcgcgaggcg ttggtcgcgt cggtggcgtg cacccgcccc ccggtcagtt
     2341 tccagatcac ccagctgtcc atcgtgccgc acagcagctc gccgcgctcc gcgcgttcgc
     2401 gggccccgtc gacgttgtcg agcagccagg cgatcttggt gccggagaag tagggatcga
     2461 gcagcaggcc ggtgacctcg gtcacccgct cctcggcgcc ggccttgacc agccgttcgc
     2521 aggcgcccgc cgttcgccgg tcctgccaga cgatcgccgg atggatcggg cggccagtgg
     2581 cgcggtccca gacgaccacc gtctcgcgct ggttggtgac gccgatcgcc gccacctcgg
     2641 ccaccgtgcg cccgcatttc ccgagcgtct cgcgcagcac cgtgacgctg gcgttccaga
     2701 tctcctcggc gtcgtgttcg acccagccgt cgtggggata gctctgtctc aggggccggc
     2761 tggcgtcggc caccgcccgg ccatgggcgt cgaaagcgat cgcccgcgtg ctggtcgttc
     2821 cctggtcaag cgccagaatt agcggatccg acatacgatt cgactcccct gccgatctca
     2881 cagtcgttta agcatgtttt cacggggtgg gagcagggtc atgcttcagc ccgactgtcg
     2941 ggacggggcc ccacgtgacc atcgccgtcg aagccgccga tctgctgctg ctggcccgga
     3001 gccggcatcc ggtcgatcgc gaacgcctgc tgaacgccat cgtcgccctg tgcgaccggg
     3061 ctgacatcga cgaggtggcg cagccgaggg tccgcgagct ggtcggctcg gtgttcatgg
     3121 ccctggtcgc cgaggccgag cgcgacatcc gccgccgact ggcggaaaag atcgccgccg
     3181 ctccctgggt tcccgccgcc ctcgtcaatg tgctggcgct ggacgatatc gagatcgccg
     3241 gaccggtgat cgccgccagc cccgtgttgc aggaccatga cctgatccgg ctgctggtcg
     3301 aggcgacgct cgatcaccag atcgccatcg cccgccgcgg ccggctcagc gccccggtgg
     3361 tcgaggccat cctgcgccag gaggagccgg ccgtcctgac cgccctggcc ggcaacgaca
     3421 ccgccgagat cacccattcg gcgatggagc ggctggtcga gacctcgcgc ggcgtcgccg
     3481 ccatgcgcac gcccctggcc cgccaccccc ggctgtcctc cgacctggcc cagcggctgt
     3541 acctgtgggt cggtcagtcg ctgcgcgcct cgctggtcga gcggttccgc ctcgacccgg
     3601 ccgagctcga cgcagccctg gccctgaccg tgcgcgaggc gcacgcc
//
LOCUS       DAORNX010000319         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19152_length_3643_cov_6.543757, whole genome shotgun sequence.
ACCESSION   DAORNX010000319 DAORNX010000000
VERSION     DAORNX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19152_length_3643_cov_6.543757"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLO65_12690"
     CDS             <1..890
                     /locus_tag="PLO65_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HRD29151.1"
                     /translation="SAFYAAVVRAAPPPEQLPPDLRGEVARAQVMSQRYAADYEAFLR
                     ERLTGLGFADDSRFARSLDLMLGKKQIYLQQPRHFYFPELPQIQFYDRAMFPWMDKVE
                     AATDAIRAELLEVMKDHAAFTPYLESRADRPFQDVHGMIDNPDWGAFYLWKNGEPVAD
                     NIARCPDTMAALADAPLARIKGRTPSVLFSLLRPGAAIPPHHGFVNTRLICHLPLIVP
                     DGCTFRVGNDVRPWREGQAWAFDDTIEHEAWNRSGETRVILLFDIWRPELTGEERAQV
                     AAMFEAIDAYGGGGGEWTV"
     gene            1018..1731
                     /locus_tag="PLO65_12695"
     CDS             1018..1731
                     /locus_tag="PLO65_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HRD29152.1"
                     /translation="MADQILMPKATAVWLVDNTSLTFEQIADFCGLHRLEVRGIADGE
                     VARDIRGADPIANGQLNREELDKAGADPKYRMVAQKSRHAELLKPVKKAPRYTPVSRR
                     QDRPDAIKWFVTHHPEVTDAQIARLLGTTKSTIESVRTRAHWNSANIKAVDPVTLGLT
                     TQIDLDAIVRKAADKKAKDDARKGIGAPDGATLKPAAETALEAEEPEIETDEEVAGHV
                     DLKAVFQDRDDEDDDGDED"
     gene            complement(1728..2258)
                     /locus_tag="PLO65_12700"
     CDS             complement(1728..2258)
                     /locus_tag="PLO65_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRD29153.1"
                     /translation="MTDDALAALRWQFDMTWALAERYHLPALTDAMCLWEPAPGAWTV
                     RPLNGVWTPDWSDVEPAPAPITSIAWTAWHLAWWWSETLARAKGAPPPGRDGVRYPGS
                     ADGVRAQLAALAGEWRPLIAGLDAGALARPSTFPWPEPRPFAMTVAWVNAELMKNVAE
                     IGAGVRLFQAAARDRS"
     gene            complement(2419..2592)
                     /locus_tag="PLO65_12705"
     CDS             complement(2419..2592)
                     /locus_tag="PLO65_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRD29154.1"
                     /translation="MALDARIRELGNRHQTLERLIEDETSRPATDDIRLKELKRQKLR
                     LKEEIESLRMQVH"
     gene            2727..3329
                     /locus_tag="PLO65_12710"
     CDS             2727..3329
                     /locus_tag="PLO65_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRD29155.1"
                     /translation="MAQTARQKLVVGISGASGVTYGLRALDACRDLGVESHLILSKAA
                     VLTLGQETDRTVAEVNAMADVVHRVGDVGAAVASGSFRALGMIIAPCSVRTMSEIATG
                     VTSSLLTRAADVTLKERRPLVLMVRETPLHLGHLRTMVRLAEMGATIAPPLPAFYAMP
                     RSIAEMVDQSVGRALDLFGLDWKPVKRWGEDLDPIIGGAA"
     gene            3326..>3643
                     /locus_tag="PLO65_12715"
     CDS             3326..>3643
                     /locus_tag="PLO65_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HRD29156.1"
                     /translation="MSFWDWMVAAYDRPGVPAATLALQDEHGFNTAFLLWAVWADPDR
                     ETLANGVQTTVLWDETVLWPLRNVRRALKGHRPPFEDAAREGLREDVKAAELRAERVL
                     AETL"
BASE COUNT          570 a         1295 c         1251 g          527 t
ORIGIN      
        1 cctcggcctt ctacgccgcc gtggtccgcg ccgccccgcc gcccgaacag ctgccgcccg
       61 acctgcgcgg cgaggtggcc cgggcccagg tgatgagcca gcgctacgcg gccgactacg
      121 aggccttcct gcgcgaacgg ctcaccggcc tgggcttcgc cgacgacagc cgcttcgccc
      181 ggtcgctcga cctgatgctg ggcaagaagc agatctatct gcagcagccc aggcacttct
      241 attttcccga gctgccgcag atccagttct acgaccgggc gatgttcccg tggatggaca
      301 aggtcgaagc cgcgaccgac gcgatccggg ccgagctgct ggaggtgatg aaggaccacg
      361 ccgcctttac accctacctc gagagccgcg ccgaccggcc gttccaggac gttcacggca
      421 tgatcgacaa tcccgactgg ggcgccttct atctgtggaa gaacggcgag cccgtcgcgg
      481 acaacatcgc ccgctgcccc gacaccatgg cggccctggc cgacgcgccc ctggcgcgga
      541 tcaagggccg cacgccgtcg gtgctgttct cgctgctgcg gccgggggcg gcgatcccgc
      601 cccaccatgg cttcgtcaac acccggctga tctgccacct gccgctgatc gtgcccgacg
      661 gctgcacctt ccgggtcggc aatgacgtac ggccctggcg cgagggccag gcctgggcct
      721 tcgacgacac catcgagcac gaggcctgga accgcagcgg cgagacgcgg gtcatcctgc
      781 tgttcgacat ctggcggcct gaactgaccg gcgaggagcg ggcccaggtg gcggccatgt
      841 tcgaggccat cgacgcctat ggcggcggtg gaggggagtg gacggtctga cgcgatttcg
      901 cgggcgttga acacgccgca gcggaacgcc gtttcctttg tcggggaacg tctctatatg
      961 tccgatccaa gacccgcccg gccgaaaggc cgggcgccgc tgtttctgga gcacgtcatg
     1021 gccgaccaaa tcctgatgcc gaaggccacc gccgtctggc tggtggacaa cacctccctg
     1081 accttcgagc agatcgccga cttctgcggc ctgcaccggc tggaggtgcg cggcatcgcc
     1141 gacggcgagg tggcgcgcga cattcgtggc gccgacccga tcgccaacgg ccagctgaac
     1201 cgcgaggagc tcgacaaggc cggggccgac ccgaaatatc gcatggtcgc ccagaagagc
     1261 cgtcacgccg agctgctcaa gccggtcaag aaggccccgc gctacacccc cgtgtcgcgc
     1321 cgccaggacc ggccggacgc gatcaagtgg ttcgtcaccc accacccgga agtgaccgac
     1381 gcccagatcg cccgcctgct gggcacgacc aagtcgacca tcgaatccgt gcgcacccgc
     1441 gcgcactgga acagcgccaa catcaaggcg gtcgaccccg tcacgctggg tctgacgacc
     1501 cagatcgacc tggacgccat cgtgcgcaag gccgccgaca agaaggccaa ggacgacgcg
     1561 cgcaagggca tcggcgcgcc cgacggggcc accctgaagc cggcggccga gaccgcgctc
     1621 gaggccgagg aaccggagat cgagaccgac gaggaagtcg ccggccacgt cgatctcaag
     1681 gccgtgttcc aggatcgcga cgacgaggat gacgacggcg acgaggacta ggaccggtcc
     1741 cgggcggccg cctggaaaag gcggacgccc gcgccgatct cggcgacgtt cttcatcagc
     1801 tcggcgttga cccaggcgac cgtcatggcg aacggccttg gctcgggcca ggggaaggtc
     1861 gagggcctcg caagggctcc ggcgtccagg ccggcgatca gcggcctcca ttccccggcc
     1921 agggccgcca gctgggcgcg cacgccgtcc gccgagcccg gatagcggac gccgtcacgc
     1981 cccggcggag gcgcgccctt cgcccgggca agggtctccg accaccacca ggccagatgc
     2041 caggcggtcc aggcgatcga ggtgatcggt gcgggggccg gttcgacgtc ggaccagtcc
     2101 ggggtccaga cgccgttcaa cggtcgcacg gtccaggcgc cgggcgcggg ctcccaaagg
     2161 cacatggcgt ccgtcagcgc cggcaggtga tagcgctcgg cgagcgccca ggtcatgtcg
     2221 aactgccacc gcaaggccgc gagggcgtca tcggtcatcg gcggcgcctc gcgtcgccgc
     2281 cgtgacccgg gatgcaggtt gcggcgcggg ccggcctctc ggcccgaccc gaattgccca
     2341 cgaccggccc gccgcgcttc atgcctcgcg gcggggggat tatggcggag ccggtccccc
     2401 gactccgata ccggtggatc agtgaacctg catccgcagg ctttcgatct cttccttcaa
     2461 gcgcagcttc tgtcgcttga gctccttcag gcggatatcg tccgtggcgg gcctactggt
     2521 ctcgtcctcg atcaaccgct cgagagtctg atgacggttg ccgagttcgc gaatgcgggc
     2581 gtccaacgcc attagtcgcc tcctcttcta ggaccctgac agtgaacacc cgccgggccg
     2641 gactgtcaga tcacatatcg ttgcgcggtc cggggagagc cgtgcgggct tccgcgcgcg
     2701 cgcgcggacg cgtagaggat ttgaagatgg ctcagaccgc gcgtcagaaa ctggttgtcg
     2761 ggatttcggg cgcctccggc gtgacctacg gcctgcgcgc gctcgacgcc tgtcgcgacc
     2821 tgggcgtcga aagccatctg atcctctcga aggccgccgt cctcaccctg ggccaggaga
     2881 ccgaccgcac ggtcgccgag gtcaacgcca tggccgacgt cgtgcaccgc gtcggcgacg
     2941 tcggagcggc ggtggcctcg ggctcgttcc gggccctggg catgatcatc gccccctgct
     3001 cggtgcggac catgagcgag atcgccaccg gcgtgacctc gtccctgctg acccgggccg
     3061 ccgatgtcac gctcaaggag cgccgtccgc tggtgctgat ggtgcgcgag acgccgctgc
     3121 acctgggcca cctgcgaacc atggtccggc tggccgagat gggcgcgacg atcgccccgc
     3181 ccctgccggc cttctacgcc atgccccggt cgatcgccga gatggtcgac cagtcggtcg
     3241 gccgcgcgct ggacctgttc gggctggact ggaagccggt gaagcgctgg ggtgaggacc
     3301 tcgatccgat catcggcgga gccgcatgag tttctgggac tggatggtgg cggcctacga
     3361 ccgtcccggt gtgccggcgg cgaccctggc gcttcaggac gagcacggct tcaacacggc
     3421 cttcctgctg tgggcggtat gggccgaccc tgaccgcgag accctggcca acggggtgca
     3481 gaccacggtg ctttgggacg agaccgtgct ctggccgctg cgcaatgtcc gccgggcgct
     3541 caagggccac cgccctccgt tcgaggacgc cgcccgcgaa gggctgcgcg aggacgtcaa
     3601 ggccgccgag ctgcgcgccg agcgggtgct ggccgagacg ctc
//
LOCUS       DAORNX010000320         3640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19168_length_3640_cov_5.634031, whole genome shotgun sequence.
ACCESSION   DAORNX010000320 DAORNX010000000
VERSION     DAORNX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19168_length_3640_cov_5.634031"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLO65_12720"
     CDS             <1..814
                     /locus_tag="PLO65_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRD29157.1"
                     /translation="VAQGVANVASAAFGGLPATGAIARTATNVRTGARSPVAGMAHAL
                     FILLFMLVLAPVMKFVPLAALAAILVVVALNMAEIHRFRLILGTTHGDRAVLLATFAL
                     TVLVDLTVAIEVGMVMAAFLFMHRMAGLASTDGGEPVIEEDREDSRSRESRYAPNFGL
                     PADVAVITFRGPLFFGSASVLKDAFDQIGARRRVYILRLDETPMVDPTGAFALRDFLK
                     RVMDDGSEVIVSGASRTVMRSLLRTIEADVLRRARFVASFETARARAVETPA"
     gene            complement(872..1291)
                     /gene="ndk"
                     /locus_tag="PLO65_12725"
     CDS             complement(872..1291)
                     /gene="ndk"
                     /locus_tag="PLO65_12725"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRD29158.1"
                     /translation="MTERTFSIIKPDATRRNLTGAVNAVIEGAGLRIVAQKRIKLTED
                     QAKKFYEVHAARPFYGELVGQMTAEPVVVQVLEGDNAVARYREVMGATNPEQAAEGTI
                     RKLFALSIGENSVHGSDSLENAAIEIAQFFTEADIVG"
     gene            1401..>3270
                     /locus_tag="PLO65_12730"
     CDS             1401..>3270
                     /locus_tag="PLO65_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD29159.1"
                     /translation="MLQINDLTFDAWGRRFFDHAGVSLPPGAKVGLVGRNGVGKSTLF
                     KLIMGELQAGGDEISLPKNTRIGQVAQEHPATPVTLIDTILEADEERHDLLTRLETAD
                     PEEMGDIWTRLIEIDSDAAPAKAAEILAGLGFSTEDLARPMAEFSGGWRMRVALAAAL
                     FSEPDLLLLDEPTNYLDLEGALWLEARLKKYPHTALIISHDRELLNNSCTHILHLAQG
                     KLEIYTGGYDAFEKMRAEKSRLQLSAKAKQDAERAHLQAFVDRFRAKASKAAQAQSRM
                     KRLAKMEPVATTIEERVAPFTLPSPPRPLAPPLIRLEKANVGYEDGKPILRNLNLRMD
                     LDDRIGLLGVNGAGKSTFAKLIAGALGVQTGEFHRDRKMRVGWFHQHQIEAMDPDDTP
                     LEIIRRAMPDASESSRRSRLAQFGLGFEKQETTVANLSGGERARLLLNLVAMDAPHML
                     ILDEPTNHLDIDSRRALLDALNDYEGAVILITHDRSLMELVADRLWLAADGTIKPFNG
                     DMDDYARFVIDKARVAARAPSQVKAPEPEPVKAPPPPPRPSAGQAAPLKRKLEAAEAV
                     LARTTRALAEIDTVLADPRVYVNNPAQAADLGQKRLKAQASVDKAEAEWMAIAEQLA"
     assembly_gap    3271..3367
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3368..>3640)
                     /locus_tag="PLO65_12735"
     CDS             complement(<3368..>3640)
                     /locus_tag="PLO65_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD29160.1"
                     /translation="NATASPPPGPPPAAPLAYGQAIAQRSCGGCHAVADGPSPLAAAP
                     PFRDLHRRYPVGGLAQLLDEGMLPPDRPLEEGSPATHPRMPVAALGA"
BASE COUNT          548 a         1233 c         1233 g          529 t
ORIGIN      
        1 ggtcgcccag ggcgtggcca atgtcgcctc ggccgcgttc ggcggcctgc ccgccaccgg
       61 cgcgatcgcc cgcacggcga ccaatgtgcg caccggcgcg cgatcgcccg tcgccggcat
      121 ggcgcacgcg ctgttcatcc tgctgttcat gctggtgctg gcgccggtga tgaagttcgt
      181 gccgctggcc gccctggccg ccatcctggt ggtggtggcg ctcaacatgg ccgagattca
      241 tcgcttccgg ctgatcctcg ggaccacgca cggcgaccgc gcggtgctgc tggcgacgtt
      301 cgccctgacc gtgctggtcg acctgaccgt ggccatcgag gtcggcatgg tcatggccgc
      361 cttcctgttc atgcaccgga tggccggatt ggcctcgacc gacggcggcg agccggtgat
      421 cgaggaggac cgcgaggatt cccggtcgcg cgagtcccgc tatgcgccga atttcggcct
      481 gccggcggat gtggcggtga tcaccttccg cggcccgctg ttcttcggca gcgccagcgt
      541 gctgaaggac gccttcgacc agatcggcgc ccggcggagg gtctatatcc tgaggctgga
      601 tgagacgccg atggtcgatc cgaccggggc cttcgccctg cgcgactttc tcaagcgggt
      661 gatggacgac gggtcggaag tgatcgtcag cggcgcttcg cggacggtga tgcggtcact
      721 gctgcggacg atcgaggccg acgtcctgcg gcgggcccgg ttcgtcgcat cgttcgagac
      781 ggccagggcg cgggcggtcg agaccccggc atgacaacgg ccggcccccg cgaggggacc
      841 ggccgtctga tatccgggcc ggatggccgc cctagccgac gatatcggcc tccgtaaaga
      901 actgggcgat ctcgatggcg gcgttctcga ggctgtcgga gccgtgcacc gagttctcgc
      961 cgatcgacag ggcgaacagc ttgcggatcg tgccctcggc ggcctgctcg gggttggtcg
     1021 cgcccatgac ttcgcggtag cgggccacgg cgttgtcgcc ttccagcacc tggacgacca
     1081 ccggctcggc ggtcatctgg ccgaccagtt cgccgtagaa cgggcgtgcg gcgtgcactt
     1141 cgtagaactt cttcgcctgg tcttcggtca gcttgatgcg cttctgcgcc acgatgcgca
     1201 ggccggcgcc ttcgatgacg gcgttcacgg cgcccgtgag gttccggcgg gtcgcgtccg
     1261 gcttgatgat cgagaaggtg cgttcagtca tggctagttt gaagccctac acaggaggtg
     1321 gcgtttggga ggtcgcgggc ttatagcggc gggcccccga tccgccaaca cctgtcgcga
     1381 agtccttttc ccaacctgcc atgctccaga ttaacgacct gacctttgac gcctgggggc
     1441 ggcgcttctt cgaccacgcc ggcgtctccc tgccgcccgg cgccaaggtc gggctggtcg
     1501 gccgcaacgg cgtgggcaag tcgacgctgt tcaagctgat catgggcgag ctgcaggccg
     1561 gcggggacga gatcagcctg ccgaagaaca cccgcatcgg ccaggtggcg caggaacatc
     1621 ccgcgacgcc ggtgaccctg atcgacacca tcctggaagc ggacgaggaa cgtcacgacc
     1681 tgctgacccg gctggagacc gccgatccgg aggagatggg cgacatctgg acccggctga
     1741 tcgagatcga ttccgacgcg gcccccgcca aggccgccga aatcctggcg gggctcggct
     1801 tcagcaccga ggacctggcc cggccgatgg ccgagttctc aggcggatgg cgcatgcgcg
     1861 tggccctggc ggcggccctg ttcagcgaac ccgacctgct gctgctcgac gaaccgacca
     1921 attatctcga cctcgaaggc gccctgtggc tcgaggcgcg gctgaagaag tatccgcaca
     1981 ccgccctgat catcagccac gaccgcgaac tgctgaacaa cagctgcacc cacattctgc
     2041 acctggccca gggcaagctg gagatctaca ccggcggcta cgacgcgttc gagaagatgc
     2101 gcgccgagaa gtcgcgcctg cagctgtcgg ccaaggccaa gcaggacgcc gagcgggcgc
     2161 acctgcaggc cttcgtcgac cggttccggg ccaaggcctc caaggccgcc caggcgcagt
     2221 cgcggatgaa gcggctggcc aagatggagc cggtggccac gaccatcgag gaacgggtcg
     2281 cgcccttcac cctgccctcg cccccccgtc cgctggcccc gccgctgatc cggctggaga
     2341 aggccaatgt cggctacgag gacggcaagc cgatcctgcg caacctcaac cttcgcatgg
     2401 acctggacga ccgcatcggc ctgctgggcg tcaacggcgc cggcaagtcg accttcgcca
     2461 agctgatcgc cggcgcgctg ggcgtgcaga ccggcgaatt ccaccgcgac cgcaagatgc
     2521 gcgtcggctg gttccaccag caccagatcg aggccatgga cccggatgac acgccgctgg
     2581 agatcatccg gcgcgccatg ccggacgcgt ccgagtcctc gcgccgctcg cggctggccc
     2641 agttcggcct gggcttcgag aagcaggaga ccaccgtcgc caacctgtcc ggcggagagc
     2701 gcgcgcggct gctgctgaac ctggtggcga tggacgcccc gcacatgctg atcctcgacg
     2761 agccgaccaa ccacctggac atcgactcgc gccgcgccct gctggacgcg ctgaacgact
     2821 acgagggcgc ggtgatcctg atcacccacg accgctcgct gatggagctg gtcgccgacc
     2881 ggctgtggct ggcggctgac ggcacgatca agccgttcaa cggcgacatg gacgactacg
     2941 ccaggttcgt catcgacaag gcccgggtgg ccgcccgcgc gccgagccag gtcaaggcgc
     3001 ccgagccaga gccggtgaag gccccgcctc cgccgccccg cccctcggcc ggccaggccg
     3061 cgccgctgaa acgcaagttg gaggccgccg aagccgtgct ggcgcgcacg accagggcgc
     3121 tcgcggagat cgacaccgtg ctcgcggacc cgagggtcta tgtgaacaac ccggcccagg
     3181 ccgccgatct cggccagaag cgcctcaagg cccaggcgtc cgtcgacaag gccgaggctg
     3241 aatggatggc catcgccgaa cagcttgcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnngcg ccgagggcgg ccaccggcat ccgcgggtgg gtcgccggcg agccctcctc
     3421 caaaggccgg tcgggcggaa gcatgccttc gtcgaggagt tgcgccagcc cgccgacggg
     3481 atatcgccgg tgcaggtcgc gaaacggcgg cgccgcggcg agcggactgg ggccatcggc
     3541 caccgcgtga cagccgccgc agctgcgttg ggcgatcgcc tgaccatagg ccaggggcgc
     3601 cgccgggggc ggaccgggcg gtgggctcgc cgtcgcattg
//
LOCUS       DAORNX010000321         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19193_length_3636_cov_8.626641, whole genome shotgun sequence.
ACCESSION   DAORNX010000321 DAORNX010000000
VERSION     DAORNX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19193_length_3636_cov_8.626641"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO65_12740"
     CDS             complement(<1..216)
                     /locus_tag="PLO65_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29161.1"
                     /translation="MTRSILIVDDDPHIRQLLAFALTKAGLEAREAEDGEAALAAVAD
                     NPPDLVILDINMPRLNGLEVCRRLRAQG"
     gene            359..1153
                     /locus_tag="PLO65_12745"
     CDS             359..1153
                     /locus_tag="PLO65_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRD29162.1"
                     /translation="MTAPLPTPAEDAWTVAGRTFRSRLIVGTGKYKDYATNAAAAEAA
                     GAEIVTVAVRRVNISDPNQPLLMDYVKPDRFTYLPNTAGCFTGEEAVRTLRLAREAGD
                     WSLVKLEVLSDPKTLYPDMEETLKALKLLVKEGFDVMVYCTDDIVYARKLEEAGAAAI
                     MPLGAPIGSGLGIQNKVNIRLIVEQSKVPVLVDAGVGTASDATIGMELGCDGILMNTA
                     IAEAKDPIRMARAMKHAVIAGREAYLAGRMARRMYADPSSPLAGLI"
     gene            complement(1244..1942)
                     /locus_tag="PLO65_12750"
     CDS             complement(1244..1942)
                     /locus_tag="PLO65_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD29163.1"
                     /translation="MRSMIIAALAALMAACGQPQTGPAVDPAFGQKVRAYLLANPEIL
                     IEVSNALKTKQARESIGAYRKQLERDPRDHVLNPQGQITVVELFDYNCGYCKLIAPEV
                     LELARTHPQARFVFKDMVIFGETSEYTAAATALARTPEQFISLHKAFMAARPLNDAVA
                     TRIITEHGLDPAAVRREQASAARKRYFQDVHGIATGLGIEGTPAFIVGDTLIPGADPD
                     ALKAAIAAELRKKG"
     gene            complement(1996..3408)
                     /locus_tag="PLO65_12755"
     CDS             complement(1996..3408)
                     /locus_tag="PLO65_12755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRD29164.1"
                     /translation="MTSPFRKPFQTVGLVAAFTAAMMAPVAPALAQSSDRGPTLLRDT
                     EIEEILHHEADPIFRVAGLNPRDVRIMLIGDPTLNAFATQGQQMGLHTGLILEAQNPN
                     ELKGVIAHETGHLAGGHPIRSGELMRAGMRPMLLTMGLGVLAMFAGAPDAGALLMANS
                     QYFGTLGALGYSREQEGRADQAAVGFLEKTGQSGQGLVEFFDRFRYQEVFSQERRFPY
                     FRSHPLSSQRIELLRTRVEAQPSYKVTDSAADILEFEIMKAKIDGFVNPQLSLTKYKE
                     TDTSYPARYARAIAYYQTKEPDRAIKLIDALLVDQPLNPYLWELKGQILFEFGRVAEA
                     EEPQRKSVELKPDAPLLRINLGQTLINLPDPKKRDEGIAELKRVTLLEDDNAEAWRLL
                     ALAYEARGDDGLARLATAEQYFALGALSESRNFAIRARELFDRQSVEWLRATDIVMAS
                     NPSDQDLRVLSEEGSASRRR"
BASE COUNT          531 a         1183 c         1322 g          600 t
ORIGIN      
        1 gccctgggcc cgcagccggc ggcagacctc cagcccgttc agccggggca tgttgatgtc
       61 gaggatcacc aggtccggcg gattgtcggc caccgccgcc agcgccgcct cgccgtcctc
      121 ggcctcgcgc gcctcgagcc cggccttggt cagggcgaag gccagcagct ggcggatgtg
      181 cgggtcgtcg tcgacgatca ggatcgaacg ggtcatgacg acattttcgc cccgtctcgc
      241 gccgggtcaa cggggcaggg gtgcaggggg cgaacgcctg tgatgcaggt tcgatgcagg
      301 cggggcggcg actctgtacc gccttccccg atgccgccgc ccggcgtaaa ccgcccccat
      361 gaccgcgccc cttccgactc ccgccgaaga cgcctggacc gtcgccggcc gcaccttccg
      421 ctcgcgcctg atcgtcggca cgggaaagta caaggactac gccaccaacg ccgccgcggc
      481 ggaggcggcc ggggccgaga tcgtcactgt cgccgtgcgg cgggtgaaca tctccgatcc
      541 gaaccagccg ctgctgatgg actatgtgaa gcccgaccgg ttcacctacc tgcccaacac
      601 ggcgggctgc ttcaccggcg aggaggccgt gcggaccctg cgcttggcgc gcgaggccgg
      661 cgactggtcg ctggtcaagc tggaggtcct gtccgacccg aagaccctct atccggacat
      721 ggaagagacg ctgaaggcat tgaaactgct cgtcaaagag ggcttcgacg tgatggtcta
      781 ctgcaccgac gacatcgtct acgcccgcaa gctggaggag gccggggccg ccgccatcat
      841 gccgctgggc gcgccgatcg gctcgggcct gggcatccag aacaaggtca acatccgcct
      901 gatcgtcgag cagtcgaagg tgccggtgct ggtcgacgcg ggcgtcggca cggcttcgga
      961 cgcgaccatc ggcatggagc tgggctgcga cggcatcctg atgaacacgg ccatcgccga
     1021 ggcgaaggac ccgatccgca tggcccgggc gatgaaacac gcggtgatcg cagggcgcga
     1081 ggcctatctg gccggccgca tggcgcgccg gatgtacgcc gatccgtcgt caccgctcgc
     1141 cgggctgatc tagcgtcccg gatttgtccc ggtttgtcgc gacagtatcg gtcccgcgag
     1201 tcctcattgg gccgcggatc cggtgtgccc ggccaggcgg cgcctagccc ttcttgcgca
     1261 actcggcggc gatggccgcc ttgagggcgt cagggtcggc gccggggatc agtgtgtcac
     1321 ccacgatgaa ggccggggtg ccctcgatgc ccaggccggt ggcgatcccg tgcacgtcct
     1381 ggaagtaacg cttgcgcgcg gcggaggcct gttcgcgccg gaccgcggcc gggtccaggc
     1441 catgctcggt gatgatccgg gtggcgaccg catcgttgag cggccgggcg gccatgaagg
     1501 ccttgtgcag gctgatgaac tgctcggggg tgcgggccag ggcggtggca gcggccgtgt
     1561 attcgctggt ctcgccgaag atgaccatgt ccttgaacac gaagcgcgcc tgcgggtggg
     1621 tgcgggccag ctccagaacc tccggggcga tcagcttgca gtagccgcaa ttgtagtcga
     1681 acagctcgac cacggtgatc tggccctgcg gattgagtac gtggtcgcgc ggatcacgtt
     1741 cgagctgctt gcgataggcg ccgatggatt cccgcgcctg cttggtcttc agcgcattgc
     1801 tgacctcaat caggatttcc ggattggcca gcagataggc ccgcaccttc tggccgaacg
     1861 ccgggtcgac cgccggcccg gtctgcggct gaccgcaggc ggccatcagg gcggctaggg
     1921 cggcgatgat catcgaacgc atcggggaac tccggcgggg cagggcggtc taggtgacgt
     1981 ggtggcggtc aggcgtcaac ggcgacgcga ggcagaccct tcctcggaca ggacccgcag
     2041 gtcctggtcg ctcgggttcg aggccatgac aatgtcggtg gcccggagcc attcgacgct
     2101 ctggcggtcg aacagctcgc gcgcgcggat ggcgaagttg cgcgactcgc tgagcgcgcc
     2161 gagggcgaaa tactgctcgg cggtggccag gcgggccagg ccgtcgtcgc cacgcgcctc
     2221 gtaggccagg gccagcaggc gccaggcctc ggcgttgtcg tcctccagca gggtgacccg
     2281 cttgagctcg gcgatgccct cgtcgcgctt cttcggatcg ggcaggttga tcagggtctg
     2341 gcccaggttg atgcgcagca gcggcgcgtc gggcttgagc tcgaccgact tgcgctgcgg
     2401 ctcctcggcc tcggcgacgc ggccgaactc gaacaggatc tggcccttca gttcccacag
     2461 gtagggattg agcggctggt cgacgagcag cgcgtcgatc agcttgatcg cccggtcagg
     2521 ctccttggtc tggtagtagg cgatcgcccg ggcgtagcgc gccgggtagc tggtgtcggt
     2581 ttccttgtat ttggtcagcg acagctgcgg gttcacgaag ccgtcgatct tggccttcat
     2641 gatctcgaat tcgaggatat cggcggccga gtccgtgacc ttgtagctgg gttgggcctc
     2701 gacacgcgtg cgcagcagct cgatccgctg cgacgacagc gggtggctgc ggaaataggg
     2761 gaagcgccgc tcctgggaga agacctcctg gtagcggaag cggtcgaaga actcgaccag
     2821 gccctggccg gactggccgg tcttctcgag gaagccgacc gccgcctggt cggcgcggcc
     2881 ttcctgttcg cggctgtagc ccagggcgcc gagggtgccg aaatactgtg aattggccat
     2941 cagcagggcg ccggcgtccg gcgctccggc gaacatcgcc agcacgccca gccccatggt
     3001 cagaagcatc ggccgcattc cggcccgcat cagctcaccg gagcggatcg gatgcccgcc
     3061 cgcgaggtgg ccggtctcgt gggcgatcac ccccttgagt tcgttggggt tctgcgcctc
     3121 gagaatcaga cctgtatgca ggcccatctg ctggccctgg gtggcgaagg cgttgagcgt
     3181 cgggtcgccg atcagcatga tccgcacgtc gcgcggattg agccccgcca cgcggaagat
     3241 cgggtcggcc tcgtggtgga ggatttcctc gatctcggta tcgcgcagca gggtcggtcc
     3301 gcggtccgag gactgggcca gggcgggggc gaccggggcc atcatcgccg cggtgaaggc
     3361 ggcgacgaga ccgacggtct ggaacggctt ccggaaaggg gaggtcatgc ctcgatacgc
     3421 tccgtcgcgc ccccgcgccc gctcaatgta gcatcggtca gccgcggcgc caccagcctc
     3481 gcttgggctt ttcgggcggg gcgaggattt cggccggatc gggccgggcc ggggtcggct
     3541 cgaccgcggg ttcgggctcg ggctcgggcg atgcggccac cggttccgtt tgcggcgcgg
     3601 gggtcggcgc ctcggcgacg accttcgctt cggggg
//
LOCUS       DAORNX010000322         3620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19321_length_3620_cov_6.725105, whole genome shotgun sequence.
ACCESSION   DAORNX010000322 DAORNX010000000
VERSION     DAORNX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_3620_cov_6.725105"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLO65_12760"
     CDS             complement(<1..422)
                     /locus_tag="PLO65_12760"
                     /inference="COORDINATES: protein motif:HMM:NF039954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HRD29165.1"
                     /translation="MPGQPLHLRITLVTPPAGVLYSLQKDDAPVGQAMSDGGDLSLDV
                     TVSFVEDDRGVRYLGPFVKNQGGRRFVYYRVGTSAGQHDSPWSRRGKVWFEDLPVDLA
                     REAAATGRRLEARFPGVAKDGGPSCATVRPIGGWRVIG"
     gene            complement(480..932)
                     /locus_tag="PLO65_12765"
     CDS             complement(480..932)
                     /locus_tag="PLO65_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD29166.1"
                     /translation="MDAKDRQIIRALQEDGRLTNQDLAERVHLSPSPCLRRLRNLEAA
                     GVITGYTALVDQKAYGLPITVFIRIRLERHSEDAVKTFETRIDRIDEVLDCYLLAGGD
                     DYLLRVIVASLEAYEDFIRRRIHAIPGIASIDTSFAYGVVKQTRLFPA"
     gene            1044..2249
                     /locus_tag="PLO65_12770"
     CDS             1044..2249
                     /locus_tag="PLO65_12770"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HRD29167.1"
                     /translation="MSAADAPGLATRAIHHGYDPMDAQGALTPPLHLTSTFAFETAEA
                     GGELFAGAREGHIYSRISNPTTHLLEQRIASLEGAEAGLATASGMGAITAVMWTFLSP
                     GDEVITDQTLYGCTFSFLRHGLARFGITVTHVDLTDPAALAAAISPRTRVVYFETPAN
                     PNMRLVDIAAVSAIARAAGARTVVDNTYATPVLTRPVELGADIVVHSATKYLGGHGDL
                     IGGLIAGGAEDLTRVRAEGVKDMTGAVMAPFNAFLILRGLKTLQVRLERHCRSAMSVA
                     RMLEAHPAVAAVHYPGLERFAQHALALRQMPGFGGMIAFELHGGFAAGAAMMNRLTLI
                     RRAVSLGDAESLIQHPASMTHSTYTAEERHAHGIADGLVRLSVGLEDEPDIIADLLRA
                     LPHHQRVAA"
     gene            2345..2812
                     /locus_tag="PLO65_12775"
     CDS             2345..2812
                     /locus_tag="PLO65_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD29168.1"
                     /translation="MPKIDLATAPRRSGSGYPPPLNEPCQKRSWVELAAAAGLDLVGI
                     NLLTLPAGQWSSQRHWHADEDEFVWVVSGEVVLVEDGGETVLRAGDCAAWKAGVANGH
                     HLQNRSGTDAVLLQIGNPTNEATEWVDYPDIDLRIHPGGEGYRHKDGTLYPAR"
     gene            complement(2844..>3620)
                     /locus_tag="PLO65_12780"
     CDS             complement(2844..>3620)
                     /locus_tag="PLO65_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29169.1"
                     /translation="EARLARARADMAATPEKLAPVRALMSSLRALGRYDEAIQVGQAL
                     RARLQDGEAFDDREEQGDWVLIQLGHSLLDTGDVAGAEAVFDEAIGSDDDRGASTDAR
                     MNWAGRLLDLSRPRDVLKVLDGLDLDYVTPYGEAWVDSQKTCALNDTDPAAAATLLES
                     LRKRKAENPGALSQALICMNRLDEAAALLIWRLQTPEHRSGGLDPFWIARPPPVVQPW
                     LARFESRRQALLSRPDVRAALDKVGRPVTTPLGGDYWGGF"
BASE COUNT          527 a         1295 c         1258 g          540 t
ORIGIN      
        1 tagccgatca cccgccagcc gccgatcggg cggaccgtgg cgcaggacgg gccgccgtcc
       61 ttcgcgacac ccgggaagcg cgcctcgagc cggcgaccgg tggcggcggc ctcgcgcgcc
      121 aggtcgaccg gcaggtcctc gaaccacacc tttccccggc ggctccaggg cgagtcgtgc
      181 tgaccggcgc tggttccgac ccggtagtag acgaaccgcc gcccgccctg gttcttcacg
      241 aacgggccca ggtaccgcac gcccctgtcg tcctcgacga agctgactgt gacatcgagc
      301 gacaggtcgc cgccgtcgga catcgcctgg ccgaccggcg cgtcgtcctt ctgcagggaa
      361 tacagcaccc cggccggcgg ggtgacgagg gtgatgcgta gatggagggg ctgcccgggc
      421 atggggccag actacaccgg ccgggaccgt tgtcgcctgg cgcggttcgc gcgacgggat
      481 caggccggaa acagccgcgt ctgcttgacc acgccatagg cgaagctggt gtcgatcgag
      541 gcgatgcccg ggatggcgtg gatgcggcga cggatgaagt cctcgtaggc ctccaggctg
      601 gcgacgatca cccgcagcag atagtcgtcg ccgcccgcca gcaggtagca gtcgagcact
      661 tcgtcgatgc ggtcgatgcg ggtctcgaag gtcttcaccg cgtcctccga atggcgttcc
      721 agccggatgc ggatgaagac ggtgatcggc aggccgtagg ccttctggtc gaccagggcg
      781 gtgtagccgg tgatgacgcc agccgcctcg agattgcgca gccgccgcag gcagggcgac
      841 ggcgaaaggt ggacccgttc ggccagatcc tggttggtca gccgtccatc ttcctgcagc
      901 gcgcggatga tctggcggtc cttggcgtcc atttcggcgc tccgattggc agaatctgcc
      961 aatcagtatg ctcagagcgg aataagtcgc aaccatccgt ctccgtggca ggcgcattat
     1021 ggcggcccat caggagttcg cccatgtccg ccgccgacgc cccgggtctc gccacccgcg
     1081 ccattcacca cggctacgat ccgatggacg cgcagggggc cctgaccccg ccgctgcatc
     1141 tgacctcgac gttcgcgttc gaaaccgccg aggccggcgg agagctgttc gccggcgccc
     1201 gcgagggtca catctattcg cgcatttcca atccgacgac gcacctgctc gagcagcgca
     1261 tcgccagtct ggaaggggcg gaggcgggcc tcgccacggc ctcgggcatg ggggcgatca
     1321 cggcggtgat gtggaccttc ctgtcgcctg gcgacgaggt gatcaccgac cagaccctgt
     1381 acggctgcac cttctctttc ctgcgacacg ggctggcccg tttcggcatc accgtgaccc
     1441 atgtcgacct gaccgatccg gcggccctgg cggcggccat ctcgccgcgc acgcgggtcg
     1501 tctatttcga gaccccggcc aatccgaaca tgcggctggt ggacatcgcc gccgtatcgg
     1561 ccatcgcacg cgccgccggc gcgcgcacgg tcgtcgacaa cacctacgcc acgcccgtgc
     1621 tgacccggcc ggtcgaactg ggcgccgaca tcgtcgtcca ctccgccacc aagtatctcg
     1681 gcggccacgg cgacctgatc ggcggcctga tcgcgggcgg ggcggaggat ctgacccggg
     1741 ttcgcgccga gggggtcaag gacatgaccg gcgcggtcat ggcgccgttc aacgccttcc
     1801 tgatcctgcg cggcctcaag accctgcagg tccggctgga acgccactgc cggtcggcga
     1861 tgagcgtcgc ccgaatgctg gaggcccatc ccgccgtggc cgccgtgcat tatcccgggc
     1921 tggagcgctt cgcccagcat gcgctggccc tgcgccagat gcccggcttc ggcggcatga
     1981 tcgcgtttga actgcacggc gggttcgccg ccggggcggc gatgatgaac cggctgaccc
     2041 tgatccgccg cgcggtctcg ctgggcgacg ccgagagcct gatccagcac cccgccagca
     2101 tgacccactc cacctacacc gccgaggagc gccacgccca cggcatcgcc gacgggctgg
     2161 tgcggctttc ggtcggcctg gaagacgagc ccgacatcat cgccgatctg ctccgcgctc
     2221 tgccgcatca ccagcgggtg gccgcctgat cctcctgctg acaggggtct gtcagcaggg
     2281 tttgcgcgcc ggttttccaa tgcgccggtt cggctaggat cggcgtgaac gccggagccg
     2341 cgccatgccg aagatcgacc tcgccaccgc cccccgccgc agcggctccg gctatccgcc
     2401 gccgctgaac gagccgtgcc agaaacggtc gtgggtcgag ctggccgccg ccgccggtct
     2461 cgacctggtc ggcatcaacc ttctgaccct gccggccggg cagtggtcga gccagcggca
     2521 ctggcacgcc gacgaggacg agttcgtctg ggtcgtctcg ggcgaggtgg tgctggtcga
     2581 ggacgggggc gagacggtgc tgcgggccgg cgactgcgcg gcctggaagg ccggcgtggc
     2641 gaacggccat cacctgcaga accgcagcgg aaccgacgcc gtgctgctgc agatcggcaa
     2701 cccgaccaac gaagctaccg agtgggtcga ctatcccgac atcgacctgc gcatccaccc
     2761 gggcggcgaa ggctaccgcc acaaggacgg gacgctgtat ccggcgcggt agggcgtccc
     2821 gcgcgcggcg ccgggtttcc gcctcagaac ccgccccagt agtcgccgcc gagcggggtg
     2881 gtgaccggcc tgccgacctt gtcgagagcg gcccggacgt ccgggcgcga gagcagcgcc
     2941 tgccggcggc tctcgaaccg cgccagccag ggctggacca ccggtggcgg gcgagcgatc
     3001 cagaacgggt cgagaccgcc cgaccggtgc tcgggggtct gcaggcgcca gatcagcagg
     3061 gcggccgcct cgtcgagccg gttcatgcag atcagcgcct ggctcagggc ccccgggttc
     3121 tcggctttcc gcttgcgcag gctttcgagc agcgtcgcgg cggcggccgg atcggtgtcg
     3181 ttcagggcgc aggtcttctg ggagtcgacc caggcctcgc cgtagggcgt gacatagtcg
     3241 aggtcgagac cgtcgagcac cttcagcacg tcgcgcgggc gcgacaggtc gagcagccgc
     3301 ccggcccagt tcatgcgggc gtcggtcgag gcgccgcgat cgtcgtccga gccgattgcc
     3361 tcgtcgaaca ccgcctcggc cccggccacg tcgccggtgt ccagcaggct gtggccgagc
     3421 tggatcagca cccagtcgcc ctgctcctcg cgatcgtcga aggcctcgcc gtcctgcagc
     3481 cgggcgcgca acgcctggcc gacctggatc gcctcgtcgt atcgaccgag cgcccgcagg
     3541 gacgacatca gcgcccgcac cggcgccagc ttttcggggg ttgccgccat gtcggcccgg
     3601 gcgcgggcca gccgggcctc
//
LOCUS       DAORNX010000323         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19568_length_3588_cov_5.356920, whole genome shotgun sequence.
ACCESSION   DAORNX010000323 DAORNX010000000
VERSION     DAORNX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19568_length_3588_cov_5.356920"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLO65_12785"
     CDS             <1..192
                     /locus_tag="PLO65_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29170.1"
                     /translation="LSAEFPIRRVADQTLTLYRALLRSEGPAATRRATRGIPAARPDN
                     AGRDVAGEQGEGLPGPPAT"
     gene            complement(284..>1141)
                     /locus_tag="PLO65_12790"
     CDS             complement(284..>1141)
                     /locus_tag="PLO65_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29171.1"
                     /translation="SVGAVLLVAGLVLAGLCGLWWLGGREPVYRISRGEPVWFVATRG
                     AGTPALAPGVRINWAARPDFGFVGPADPWWDRVLLLSGGAVGQPPVAFGAGVDDAMIL
                     RLALMRPPRLALGLLRGLLTLGILRPPTGPVAEDLAGMGLRPDLMPDRDGIARLLARP
                     ADYRPAMVNFLRYFDAARYAEPRPDARPSTGAAAYRRYGVVAMRTVYQTRGFLAFYGQ
                     VSEIVRPAAAGPAIGTWDDVAVMQYRRPEGILTMEHVPRYRAALAHRDAGLERTLVIA
                     ATADGPAAH"
     assembly_gap    1142..1197
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1198..2316)
                     /locus_tag="PLO65_12795"
     CDS             complement(<1198..2316)
                     /locus_tag="PLO65_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRD29172.1"
                     /translation="MNPKAEFDIIVYGATGFTGRLVAEYLASRYGVGGDVRWAMAGRS
                     ADKLAQVRTEIGAPADTPLVVADAADPASLAAMVARTRAVLTTVGPYQLYGSALVAAC
                     VAAGTDYLDLSGEPTWMAEMIAAHDAAARASGARILFSCGFDSIPFELGVFFTQGLAR
                     TRLGHPVPRIKGRVRAMRGGLSGGTAASGAATMAAIQKNPALVALMFNPFALTPGFEG
                     PEQPRGDKPGLDEDLGQEVGPFMMATINTKNVHRSNLLQAHAWGRDFVYDEMAVVTQG
                     AATAFTDLGGAGAPRPGEGPGKAEREAGFYDLLFIGVDADGRQVRGAVHGDRDPGYGS
                     TSKIIAETAVCLVREAPDTPGGVWTPGAALGQKLVDRLA"
     gene            2381..>3588
                     /locus_tag="PLO65_12800"
     CDS             2381..>3588
                     /locus_tag="PLO65_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29173.1"
                     /translation="MTQVSPQGAPTPRFAAASLIAVIFINMLGFGIIVPLLPFYAKSF
                     DAPPWQIALIFSAYSVGAFFGEPFWGRLSDRYGRKPILISTIAANCLCYMALALAPNI
                     PAAFLIRLVGGLAAGNGSVIQGYIADVTPPERRARQMSYQGAAWNVGLIVGPSIGGLF
                     AHTNAGPAGFRIPLFIASGLACLCVIAIMLFIRESRVRETGYSHRPSRWAAFGEAFRH
                     PIIGRLMLLTFLVGFAFTGIESVFGLWTQARFGWEPRDIGVSFAFVGATAFLTQTLAT
                     GRLSERFGEGPMLAAGMALSVVAMSLQTVSTGGPMTTVLMCLTAVGQSVAFPNVGALI
                     SRTADPHRQGQIMGLNNAAGAMARVLGPLSASLVFQGWLNGPFMLGALVVAPAILLAL
                     AASRLARAPL"
BASE COUNT          487 a         1302 c         1233 g          510 t
ORIGIN      
        1 ctgtctgccg agtttccgat ccgccgtgtc gcggaccaga ccctgaccct gtaccgggcc
       61 ctgctgagga gcgaggggcc ggcagcgacc cggcgtgcga cgcgcggaat cccggcggcg
      121 agaccggaca acgccggaag agatgtcgcc ggcgaacagg gcgaggggtt gcccggcccg
      181 ccggcgacgt gatccgtcgc cggatcaagg tcaggctaga ccactccccg cggccgctgt
      241 gtcggcggcc cgaccgaacc aggacgatac ccgtcgaagg gcctcaatga gccgcgggtc
      301 cgtcggcggt ggcggcgatc accagggtcc gctcgagccc cgcgtcgcgg tgggcgagcg
      361 ccgcgcgata acgcgggaca tgctccatgg tcaggatgcc ctcgggccgc cggtattgca
      421 tcaccgcgac gtcgtcccag gtcccgatcg ccgggcccgc ggccgccggc cggacgatct
      481 cgctcacctg gccgtaaaag gcgaggaagc cgcgggtctg gtagaccgtg cgcatggcca
      541 ccacgccgta gcgacgatag gcggccgcgc cggttgacgg ccgcgcatcg ggtcgcggct
      601 cggcgtatcg ggcggcgtcg aagtagcgca ggaagttgac catcgccggc cggtagtcgg
      661 ccggccgggc cagaaggcgg gcgatgccgt cgcgatcggg catcagatcc gggcgcagcc
      721 ccatgccggc gaggtcctcg gccaccgggc cggtcggcgg cctcaggatg ccgagggtca
      781 gcaggccccg cagcaggccc agggcgaggc gcggcggccg catgagggcg aggcgcagga
      841 tcatcgcgtc gtcgacgcct gctccgaatg ccaccggagg ctggccgaca gcgccgcccg
      901 acagcagcag gacccggtcc caccaggggt cggccggacc gacgaagccg aaatccggac
      961 gggcggccca gttgatccgc acgcccgggg ccagggccgg cgtccctgcc ccgcgagtcg
     1021 ccacgaacca gaccggctcg ccgcggctga tgcggtagac cggctcgcga ccgccgagcc
     1081 accagaggcc gcaaagaccg gccagcacca ggccggccac caacaggact gcccccaccg
     1141 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
     1201 gagccggtcc accagcttct ggccgagcgc cgcgccggga gtccagacgc cgccgggcgt
     1261 gtccggcgct tcgcggacca ggcagacggc cgtctcggcg atgatcttcg aggtcgagcc
     1321 gtagccgggg tcacggtcgc cgtgcacggc cccgcgcacc tggcggccgt ccgcatcgac
     1381 gccgatgaac agcagatcgt agaagccggc ctcgcgctcg gccttgcccg gcccctcgcc
     1441 ggggcggggc gcgccggcgc cgcccaggtc ggtgaaggcc gtcgccgcac cctgggtcac
     1501 caccgccatc tcgtcataga cgaagtcgcg gccccaggcg tgggcctgca gcagattgga
     1561 acgatggacg ttcttggtgt tgatggtggc catcatgaac ggccccacct cctggccgag
     1621 atcctcgtca aggccgggct tgtcgccgcg cggctgttcc ggcccctcga agccgggcgt
     1681 cagggcgaag gggttgaaca tcagggcgac cagcgccgga ttcttctgga tcgccgccat
     1741 ggtcgccgcg ccgctggccg ccgtgccgcc ggacaggccg ccgcgcatcg cgcgcacccg
     1801 tcccttgatc cgcggaacgg gatggccaag tcgcgtcctg gccagcccct gggtgaagaa
     1861 cacgcccagt tcgaagggga tggaatcgaa gccgcaggag aacaggatcc gcgcgccgga
     1921 cgccctggcg gcggcgtcgt gggccgcgat catctcggcc atccaggtcg gttcgcccga
     1981 caggtccaga tagtcggtcc cggcggcgac gcaggccgcc acgagcgccg acccatagag
     2041 ctggtacggg cccaccgtcg tcaggacggc gcgggtccgg gcgaccatcg ccgccaggga
     2101 ggccgggtcg gccgcgtcgg cgacgaccag cggggtgtcg gccggcgcgc cgatctctgt
     2161 ccgcacctgg gccagcttgt cggcgctgcg accggccatc gcccaccgca cgtctccgcc
     2221 gacgccgtaa cggctggcga gatattcggc gaccagcctg ccggtgaagc cggtggcccc
     2281 atagacgatg atgtcgaact cagccttcgg attcatgcca cagctccatc gcggcacggc
     2341 ccccttgccc acccgccggg ctgtccttat caccgttcac atgacgcagg tatcgccgca
     2401 gggggcgccg acgccccgct tcgcggcggc ctcgctgatc gccgtgatct tcatcaacat
     2461 gctcggcttc gggatcatcg tcccgttgct gcccttctac gccaaatcgt tcgacgcgcc
     2521 gccctggcag atcgccctga tcttctcggc ctattcggtc ggcgccttct tcggcgagcc
     2581 cttctggggg cgactgtcgg accgctacgg ccgcaagccg atcctgatct cgacgatcgc
     2641 cgccaattgc ctttgctaca tggccctggc gctggccccg aacatcccgg cggccttcct
     2701 gatccggctg gtcggcggcc tggccgcggg caacggctcg gtcatccagg gctatatcgc
     2761 cgacgtcacg ccgcccgagc ggcgcgcccg gcagatgtcc taccagggcg cggcctggaa
     2821 cgtgggactg atcgtcggcc cgtcgatcgg cggactgttc gcccacacca acgccggtcc
     2881 ggcgggcttc cgcattccgt tgttcatcgc ctcgggcctg gcctgcctgt gcgtcatcgc
     2941 catcatgctg ttcatccgcg aaagccgggt gcgcgagacc ggctattccc atcgtcccag
     3001 ccgctgggcc gccttcggcg aggcgtttcg ccatccgatc atcggacggt tgatgctgct
     3061 caccttcctg gtcggcttcg ccttcaccgg catcgagtcg gtgttcggcc tgtggaccca
     3121 ggcgcgcttc ggctgggagc cgcgcgacat cggcgtcagc ttcgccttcg tcggggcgac
     3181 ggcgttcctg acccagaccc tggccaccgg ccgcctgtcg gagcggttcg gcgagggacc
     3241 gatgctggcg gcgggcatgg ccctgtccgt cgtggccatg agcctgcaga cggtgtcgac
     3301 cggcggcccc atgaccaccg tgctgatgtg cctgaccgcc gtgggccagt cggtggcgtt
     3361 tcccaacgtc ggggcgctga tctcgcgcac cgccgatccc catcgtcagg gccagatcat
     3421 gggcctcaac aacgccgccg gcgccatggc gcgggtgctc ggcccgctca gcgccagcct
     3481 ggtgttccag ggctggctca acggcccctt catgctcggc gcgctggtcg tggccccggc
     3541 catcctgctg gctctggccg ccagccggct ggcgcgcgcg ccgctcta
//
LOCUS       DAORNX010000324         3578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19645_length_3578_cov_7.850412, whole genome shotgun sequence.
ACCESSION   DAORNX010000324 DAORNX010000000
VERSION     DAORNX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19645_length_3578_cov_7.850412"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLO65_12805"
     CDS             <1..207
                     /locus_tag="PLO65_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD29174.1"
                     /translation="ENTKSAIRSVVASDPDIKILVLAASTGGKGPGPLVASLARDGVN
                     WGTRKLPVTVVPGDLTDEEVADLA"
     gene            204..500
                     /locus_tag="PLO65_12810"
     CDS             204..500
                     /locus_tag="PLO65_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29175.1"
                     /translation="MSEEPGFTPNSPLTRKPRKRSWLAAWVLLALVAGMALIVLVGQR
                     APAAAWRAAGVAYGVLLAFMAVRQVWLVRRDLRSRRFRDQWFGRMLDRSSLDDS"
     gene            complement(690..1985)
                     /locus_tag="PLO65_12815"
     CDS             complement(690..1985)
                     /locus_tag="PLO65_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD29176.1"
                     /translation="MADGNFDLGKALRDKAERDRVARERAYATPLDQINVVDPELWQT
                     DTHWPYFERLRKEDPVHLCTVDEETGPYWSVTRFNDIMEIDTSHQVFSSDAHLGGITL
                     RDFDEDFVLPMFIAMDPPKHDVQRKTVQPIVSPQSLMQLEPIIRERAAGLLDSLPIGE
                     TFDWVDRISIELTTQMLATLFDFPFHDRRKLTRWSDVATAAPGMGVIETEEQRREELF
                     ECLGYFTELWNQRVNAPPANNLISMLAHGEATRNMEPMEYLGNLILLIVGGNDTTRNT
                     MTGSVLALNQNPDQYDKLRANPDLIPSMVSETIRWQTPLAHMRRTALADYEIGGKTIK
                     KGDKVAMWYVSGNRDETAIENPNAYIIDRERPRHHMSFGFGIHRCVGNRLAEMQLRVV
                     WEEILKRFPTIEVVGEPERVPSAFVKGYLTLPVRIPAKL"
     gene            2110..2676
                     /locus_tag="PLO65_12820"
     CDS             2110..2676
                     /locus_tag="PLO65_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRD29177.1"
                     /translation="MFIQTEPTPNPAVLKFLPGRDVLGDGAREFRDADEAASSPLAKA
                     LFDLGDVTRVFFGGDFLTVTKDGDSDWAHLKAPVLAAIMDHFTSGAPILNDDDGTGHD
                     ATVYEGETAQIVAEIKDLLDSRIRPAVAQDGGDIVFSRFEADTGVVYLHMRGACSGCP
                     SSSATLKAGVENMLKHYVPEVTRVEQTL"
     gene            2724..3311
                     /locus_tag="PLO65_12825"
     CDS             2724..3311
                     /locus_tag="PLO65_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29178.1"
                     /translation="MVALVFAALLASPVSAAGLDPAFEAWAGDTESCSLWYRIQEETG
                     QGERRWVRVCFMHRMESEDVDPSPEWWVRRLVLTFPERGGAQAKVTRLRTDVARCPVI
                     AAIVDEAPAALDVKLRPDPGSLLGRTFKDEVTVSVGAEGVVTPIETSGSVSVSGTVAV
                     SEAGGFPPSAWAFAAEARMKGCWKTELPPPNIALP"
     gene            3302..3379
                     /locus_tag="PLO65_12830"
                     /pseudo
     CDS             3302..3379
                     /locus_tag="PLO65_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
BASE COUNT          536 a         1188 c         1225 g          629 t
ORIGIN      
        1 gagaacacca agtcggcgat tcgctcggtg gtggccagtg atccggacat caagatcctg
       61 gtcctggccg cctcgacggg cggcaagggc ccggggccgc tcgtggcctc gctcgcgcgg
      121 gacggggtca actgggggac gcgcaagctg ccggtcaccg tggtgcccgg cgatctgacc
      181 gacgaggagg tcgctgacct ggcatgagcg aggagccggg attcacgccc aattcgccgc
      241 tgacccggaa gccgcgcaag cgaagctggc tggcggcctg ggtcctgctc gccctcgtgg
      301 ccggcatggc cctgatcgtc ctggtcggtc agcgcgcgcc cgcggccgcg tggcgggccg
      361 cgggcgtggc ctacggcgtc ctgctggcct tcatggccgt caggcaggtc tggctcgtcc
      421 ggcgggacct gcggtcgcgg cgcttccggg accagtggtt cggccggatg ctcgaccgca
      481 gttcgctcga cgacagctga ccagccggcg acgcccctgc cttcctctcc cgggggagag
      541 gtggcccggt ggccgggtcg gagagggcag cgccggctgg agccggccca tcctgaattc
      601 cggcgtttgc aggcgaagac cgcgccggcc cctccaccgc cctccgggcg gtccccctcc
      661 cgcttcgcgg cgaggaagac ccgaaggttc tacagcttcg ccggaatccg cacgggcagg
      721 gtcagatagc ccttcacgaa ggccgacggg acccgctcag gctcgcccac cacctcgatg
      781 gtcgggaagc gcttgaggat ctcttcccag accacccgca gctgcatctc ggccaggcgg
      841 ttgccgacgc agcggtggat gccgaagccg aacgacatgt ggtggcgggg gcgttcgcga
      901 tcgatgatgt aggcgttcgg attctcgatc gccgtttcgt cgcggttgcc cgagacgtac
      961 cacatggcga ccttgtcgcc cttcttgatc gtcttgccgc cgatttcgta gtcggccagg
     1021 gccgtgcggc gcatgtgggc cagcggcgtc tgccagcgga tggtctcgga gaccatcgac
     1081 gggatcaggt cgggattggc gcgcagcttg tcgtactggt cgggattctg gttcagcgcc
     1141 agcaccgagc cggtcatggt gttgcgggtg gtgtcgttgc cgccgacgat cagcaggatc
     1201 aggttcccga gatactccat cggctccatg ttgcgggtcg cctcgccgtg ggcgagcatg
     1261 gagatcaggt tgttggccgg cggagcgttg acccgctggt tccacagctc ggtgaagtag
     1321 ccgaggcatt cgaacagctc ctcgcggcgc tgctcctcgg tctcgatcac ccccatgccc
     1381 ggcgcggccg tggccacgtc cgaccagcgg gtcagcttgc gccggtcgtg gaacgggaag
     1441 tcgaacagcg tggccagcat ctgggtggtc agctcgatcg agatgcggtc gacccagtcg
     1501 aaggtctcgc cgatcggcag gctgtcgagc aggccggcgg cccgctcgcg gatgatcggc
     1561 tccagctgca tcaggctctg ggggctgacg atcggctgaa ccgtcttgcg ctgcacgtcg
     1621 tgcttgggcg ggtccatggc gatgaacatc ggcaggacga agtcctcgtc gaagtcacgc
     1681 agggtgatgc cgcccaggtg ggcgtccgag ctgaacacct ggtggctggt gtcgatttcc
     1741 atgatgtcgt tgaagcgggt caccgaccag taggggccgg tttcctcgtc gaccgtgcag
     1801 aggtggaccg ggtcctcctt ccgcaggcgt tcgaaatagg gccagtgggt gtccgtctgc
     1861 cacagctcgg gatcgacgac gttgatctgg tccagcggcg tcgcataggc gcgctcgcgc
     1921 gccactcgat cgcgctcggc cttgtcccgc aacgccttgc ccagatcgaa attcccgtcg
     1981 gccatggccg tctcccttgt tttgcgccct gccgcgcccg tcagcgcgcc cgctatctta
     2041 tcgttgctta catagcgcca gaaaatggcg cccttgtcac ctgccgcgcg cagcgccaca
     2101 tgtgttttca tgtttatcca gaccgaaccc acacccaatc cggcggtgct caagttcctg
     2161 cccggccggg atgtgctcgg cgacggggcc cgcgagttcc gcgatgcgga cgaggcggcc
     2221 tcctcgccgc tcgccaaagc cctgttcgac ctcggcgacg tgacccgcgt gttcttcggc
     2281 ggcgacttcc tcaccgtgac caaggatggg gacagcgact gggcgcatct gaaggccccg
     2341 gtgctggccg cgatcatgga ccatttcacg tccggcgcgc cgatcctcaa cgacgatgac
     2401 gggaccggcc acgacgccac cgtctacgag ggcgagaccg cccagatcgt cgccgagatc
     2461 aaggacctgc tggacagccg catccgcccg gccgtggccc aggacggcgg cgacatcgtc
     2521 ttctcgcgct tcgaggccga caccggcgtg gtctacctgc acatgcgcgg cgcctgctcg
     2581 ggctgcccgt cctcctcggc gacgctgaag gccggggtcg agaacatgct caaacactat
     2641 gtgcccgaag tgacgcgggt cgagcagacg ctgtaggcgc cccgcgacaa ccagcctagc
     2701 ctgccggtat gagatgtttc acgttggtcg cgctggtctt cgccgccctc ttggcgtcgc
     2761 ccgtaagcgc ggcaggactt gatccggcct tcgaggcctg ggccggggac acagaatctt
     2821 gttcgctttg gtatcggatt caggaagaga ccggccaagg cgagcggcgt tgggtgcgcg
     2881 tgtgcttcat gcatcggatg gaatcggaag acgtcgatcc gagccccgaa tggtgggtgc
     2941 ggcgcctcgt gctcaccttc cccgagcgcg gcggggccca ggccaaggtg actcgcctgc
     3001 gcacggacgt ggcgcgctgt ccggtcatcg ccgccattgt cgatgaggcg ccggcggcgc
     3061 tggacgtgaa gctgcggccg gatcccggtt ctcttctggg ccggacgttc aaggacgagg
     3121 tcaccgtctc ggtcggcgcg gaaggcgtcg tcactccgat cgaaacatca ggctctgtca
     3181 gcgtatcagg gacagttgcg gtgtcggaag ccggaggctt tccgccgtcg gcctgggcct
     3241 tcgccgccga agcgcggatg aagggctgct ggaagactga gctgccgccg ccgaacatcg
     3301 cgctaccctg atcgccagct tggagaacat gctcaaacac tatgtgcccg aagtgacgcg
     3361 ggtcgagcag acgctgtaga acgcgcgacc gtcatcccgg gccctgtgcc cgggatccct
     3421 cgatccgtca cacgactccg attccagcct gacgctatca ggcggtgtcg acagagatcc
     3481 cgggcgcgag gcccgggatg acgacgtgtt tggttagaca gccgccatcg cctaaacagc
     3541 ccgaatgccc cgccgcttcc tcatcgcgag caccctga
//
LOCUS       DAORNX010000325         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19710_length_3567_cov_7.271071, whole genome shotgun sequence.
ACCESSION   DAORNX010000325 DAORNX010000000
VERSION     DAORNX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19710_length_3567_cov_7.271071"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /gene="queG"
                     /locus_tag="PLO65_12835"
     CDS             complement(<1..1026)
                     /gene="queG"
                     /locus_tag="PLO65_12835"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRD29179.1"
                     /translation="MTILISDAQKTRIRHGALALGFDVCGFASAADPWPAGARLEAFV
                     AEGLHGTMGWMAETLARRAHPTGMWPDARSAIVLGLNYGPDRDPLETLKARSTGAISV
                     YAQGDDYHELIKGRLKTLAGQVVARHGGEVKVFVDTAPLMEKPLAQRAGLGWQGKHTN
                     LVSRELGSWLFLGSVLTSLEIAPDPAEVDHCGSCRACLDICPTAAFPAPYRLDARRCI
                     SYLTIEHAGPVPEEFRAALGNRIYGCDDCLAVCPWNKFAAVARESAFHARDALRSPSL
                     ATLAALDDAGFRAMFRKSPVKRIGRDRFVRNVLYAIGNSGDATLAASAAALADDPSDV
                     VRDAARWA"
     gene            complement(1007..1693)
                     /locus_tag="PLO65_12840"
     CDS             complement(1007..1693)
                     /locus_tag="PLO65_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29180.1"
                     /translation="MSVERTLHHFPLDPASRQVRLALGEKRLSFTEVQVRWWESPPDF
                     AQLNPSGVPPVLVEVDGGDRVVICETRAVLEHLEEQNPEPALLGRAPAERAEARRLMQ
                     WFDRKFDAEVSGFLLHEKMEKRLLGLGAPALANIRAGRDALRLHMQYIERLLADRDWL
                     AGKRISLADMAAAAHLSVIDYFGDVPWNDFPAAKTWYMKIKSRPCFRPLLADRWPGMA
                     PAPHYHDLDF"
     gene            complement(1748..2686)
                     /locus_tag="PLO65_12845"
     CDS             complement(1748..2686)
                     /locus_tag="PLO65_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRD29181.1"
                     /translation="MTTAPLTTAQAAVLTRRVTSLSVATAIVLVAAKTAAWLASGSVA
                     LLASLADSGLDLLASLITLYAVRYAVEPPDAEHRFGHGKAEAFASLMQAGLVFASAAL
                     IGREAIAGMLEPRPIGAGGWAVAVMGLSVVLTVGLISVQSWVLRRTRSIAVTGDRAHY
                     AADLASNLAAMAGIAGATLLGLHWLDAAAGLLVALWLLWGAIGVFREAGAQLMDHELP
                     EADRARIIALMTEDPRIQDVHQLRTRASGPYIHIQMHADLDPDSSLSAAHEAVVAAEN
                     RVLAAFPAADIIIHPDPRGRAEPHGGAFGEAEHESR"
     gene            complement(2742..>3567)
                     /locus_tag="PLO65_12850"
     CDS             complement(2742..>3567)
                     /locus_tag="PLO65_12850"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRD29182.1"
                     /translation="VEAMRALTARLADGDGLDTALRLLAAPAVFTPAVVRAQAGQSLV
                     PPDPTLGHSADILRMLHGRPASAAEAGALDAYLVTVSDHGLNASTFAARVVASTQAGL
                     TSAVLAGISALKGPLHGGAPGPVIDMLDGIGRPENARAWLEAALDRGERLMGFGHRIY
                     RVRDPRADVLKAAVRRMSEGSNVLPGRVAFAEAVETAALAILRDRKPDRPLDINVEFY
                     TALLLEALAFPPSTFTGVFAMGRVAGWIAHAREQLAGGRLIRPQSQYIGPQPRRAA"
BASE COUNT          524 a         1260 c         1305 g          478 t
ORIGIN      
        1 cgcccaccgc gccgcgtccc gcacgacatc cgaggggtcg tccgccagcg ccgccgccga
       61 cgccgccagc gtcgcatccc cgctgttgcc gatggcgtag agcacgttcc tgacgaaccg
      121 gtcgcggccg atgcgcttga ccgggctctt gcggaacatc gcccggaagc cggcgtcgtc
      181 gagcgcggcc agcgtcgcca gcgagggcga ccgcagcgcg tcccgcgcat ggaaggcgct
      241 ctcgcgcgct acggccgcga acttgttcca cgggcagacc gccagacagt cgtcgcagcc
      301 gtagatgcgg ttgcccaggg ccgcgcggaa ttcctcggga accgggcccg cgtgctcgat
      361 ggtcaggtag gagatgcagc gccgcgcatc gagccggtag ggcgccggga aggccgcggt
      421 cgggcagatg tcgaggcagg cccggcagct gccgcaatgg tcgacctcgg ccggatctgg
      481 cgcgatctcc agcgacgtca ggaccgagcc gaggaacagc cagctgccga gctcgcgcga
      541 gaccaggttg gtgtgcttgc cctgccagcc gaggcccgcc cgctgcgcca gcggcttttc
      601 catcaagggg gcggtgtcga cgaacacctt cacctcgccg ccgtgccgcg ccacgacctg
      661 gccggcgagg gtcttgagcc ggcccttgat cagctcgtga tagtcgtcgc cctgcgcata
      721 gacggagatg gcgcccgtcg agcgggcctt cagggtctcc agcggatcgc ggtccggacc
      781 gtagttgagc cccagcacga tcgccgatcg ggcgtcaggc cacatgccgg ttggatgggc
      841 ccggcgtgcg agcgtctcgg ccatccagcc catggtcccg tgcaggcctt cggcgacgaa
      901 ggcctccagg cgggccccgg cgggccatgg gtccgccgcc gaggcgaacc cgcagacgtc
      961 gaatccgagg gcgagggccc cgtggcggat gcgggtcttc tgggcgtcag aaatcaagat
     1021 cgtgatagtg gggcgccggc gccatgcccg gccaacgatc ggccagaaga ggtctgaaac
     1081 agggccgcga cttgatcttc atgtaccatg tcttggccgc cggaaagtcg ttccacggca
     1141 cgtcgccgaa atagtcgatc accgacaggt gggcggcggc ggccatgtcg gccagcgaga
     1201 tgcgcttgcc ggccagccag tcgcgatcgg ccagcagccg ctcgatgtac tgcatgtgca
     1261 gcctgagggc gtcgcggccg gcgcggatgt tggccagggc cggagcgccc aggcccagca
     1321 ggcgcttctc catcttctcg tgcagcagaa agccgctgac ctcggcgtcg aacttgcggt
     1381 cgaaccactg catcagtcgg cgggcctcgg cccgttcggc cggcgcgcgg ccaagcaggg
     1441 ccggttcggg attctgttcc tcgagatgtt cgagcacggc gcgggtttcg cagatcacca
     1501 cccgatcgcc gccgtcgacc tccaccagca cgggcggtac gccggacgga ttgagctgcg
     1561 cgaaatcggg cgggctttcc caccaccgca cctgcacctc ggtgaacgac agccgcttct
     1621 cgccgagcgc cagtcgcacc tgccgcgagg ccggatcgag cgggaagtgg tgaagggtgc
     1681 gttcgacgct catcgcgggc ctggtttctg cgccgccact agacgccgag gggcgttaag
     1741 gccgcgttta ccgcgactcg tgctcggcct cgccgaacgc cccgccgtgg ggttcggccc
     1801 ggccgcgcgg atcggggtgg atgatgatgt cggctgccgg gaaggccgcc aggacccggt
     1861 tctcggccgc cactacggcc tcgtgcgccg ccgacaggct gctgtcggga tcgaggtcgg
     1921 cgtgcatctg gatatggatg taggggcccg aggcccgggt gcgcagttgg tgcacgtcct
     1981 ggatccgggg atcctcggtc atcagggcga tgatcctggc ccggtcggct tcgggcagtt
     2041 catgatccat cagctgggcg ccggcctcgc ggaacacgcc gatggcgccc cacagcagcc
     2101 agagggccac cagcaagccg gccgccgcgt ccagccagtg caggcccagc agcgtcgccc
     2161 cggcgatgcc cgccatggcc gcgaggttcg aggccaggtc ggcggcgtag tgagcccggt
     2221 caccggtgac ggcgatcgac cgcgtgcgcc gcaggaccca ggactggacc gagatcaggc
     2281 cgaccgtcag gaccaccgac aggcccatca ccgcgacggc ccagccgccc gcgccgatcg
     2341 gtctgggctc gagcatgccg gcgatggcct cgcggccaat cagggccgcc gaggcgaaca
     2401 ccagacccgc ctgcatcagg ctggcgaagg cctcggcctt gccgtggccg aagcggtgct
     2461 cggcatcggg cggctcgacg gcgtagcgca ccgcgtagag ggtgatcagc gacgccagca
     2521 ggtcgaggcc ggaatcggcc agtgacgcca gcagagccac cgagccgctg gccagccatg
     2581 ccgccgtctt ggccgcgacc agcacgatcg cggtggccac cgacaggctg gtcacccggc
     2641 gcgtcagaac ggcggcctgg gccgtggtca ggggagcggt cgtcatggcg tcgttctagc
     2701 cgcccagcgc gtcggacccc agcgcgaacg attctcgcct ttcaggccgc ccggcgcggc
     2761 tgcgggccga tgtactgcga ctgcgggcgg atcaggcggc cgccggccag ctgctcccgg
     2821 gcatgggcga tccagccggc cacgcggccc atggcgaaca cgccggtgaa ggtcgacggg
     2881 gggaaggcca gggcctcaag cagcagcgcg gtgtagaact cgacattgat gtccagcggc
     2941 cggtccggct tgcggtcccg cagaatggcc agggcggccg tctccacggc ctcggcgaag
     3001 gcgacgcggc cgggcaggac attggaccct tcgctcatcc gccgcaccgc ggccttgagc
     3061 acgtcggccc gcggatcgcg gacgcgatag atccggtggc cgaagcccat cagccgttcg
     3121 ccccggtcga gcgccgcctc cagccaggcg cgggcgttct ccggccggcc gatgccgtcg
     3181 agcatgtcga tcaccgggcc gggcgcgccg ccgtgcagcg ggcccttgag cgccgagatt
     3241 ccggccagta cggccgaggt caggccggcc tgggtcgagg ccaccacgcg cgcggcgaag
     3301 gtcgaggcgt tcagcccgtg atcgctgacc gtgaccagat aggcgtcgag cgccccggcc
     3361 tcggccgcgc tggccggccg gccgtgcagc atgcgcagga tgtcggcgct gtggccaagg
     3421 gtcgggtcgg gcgggaccag cgactggccg gcctgggcgc ggaccaccgc gggcgtgaac
     3481 accgcgggcg cggccagcag cctcaacgcc gtatcgagcc cgtcgccgtc ggcgagccgg
     3541 gccgtcaggg cgcgcatggc ctcgacc
//
LOCUS       DAORNX010000326         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19828_length_3549_cov_8.546938, whole genome shotgun sequence.
ACCESSION   DAORNX010000326 DAORNX010000000
VERSION     DAORNX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19828_length_3549_cov_8.546938"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLO65_12855"
     CDS             <1..334
                     /locus_tag="PLO65_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29183.1"
                     /translation="DARARAWLDINCGHCHSPRGPADTSGLYLDAASPAGASLGLCKP
                     PVAAGQGTGGFSHDIEPGRPDESILMFRTGSTDPGAMMPELGRSLAHAEGLALLRRWI
                     AAMPSGCR"
     gene            334..1329
                     /locus_tag="PLO65_12860"
     CDS             334..1329
                     /locus_tag="PLO65_12860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRD29184.1"
                     /translation="MPPPGGPDRTWLSIQHLRGLAALSVALFHACQWSQVDFATGQAG
                     VDVFFVISGFVMWTATSGRAVRPARFLLRRLIRVAPLYWLITLALAGGVLLFPARFPD
                     LAVTPAHLLLSLAFVQHMNPAGQPFPLLAPGWTLDYEAAFYLVFAALLVLPEGRRLFA
                     LTVALVVLAFAGFLWPPAYQMLLNPLFLEFLAGLWLGRMAQLQLLPDRSLGWLMLGGA
                     VGLFILIQLVNLDWDLWRPMVWGVPAALLVAGAVSIEADGGWKAVPGLQTLGDASYST
                     YLIHTLTIGALAMTIGAWNPPLFIPLAMIVAVVSGVGLWALIERPMLDGLRRRLA"
     gene            1400..3511
                     /locus_tag="PLO65_12865"
     CDS             1400..3511
                     /locus_tag="PLO65_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HRD29185.1"
                     /translation="MRRISALAVTLAAAAMLAGCGKKAADIDGARIAAADVNPAEWVT
                     HGRTYGEQRFSPLDKVNVGNVGQLGLAWSYDLPENRGVEATPLVADGVMYTTSSWSIV
                     RAFDAKTGKLLWEFDPKVPRKTGVKACCDSVNRGVALWEGRVYFGALDGRLIALDART
                     GKQAWSVVTVDQSKPYTITGAPRVIKGKVLIGNGGAELGVRGYLSAYDARNGKMLWRF
                     FTVPGEQATDGAASDAAMKIARPTWKGEFWKVGGGGTVWDSMAYDPDLDLLYIGVGNG
                     SPWNRDIRSPGGGDNLFLSSIVALKPDTGQYVWHYQTTPGESWDYTATQHIILADLTI
                     GGKTVKALMQAPKNGFFYVLDRTNGKLISAKNYVPQTWATGVDPATGRPIETPNARYG
                     SGPSLAMPGPLGGHNWQPMAYSPRTNLVYIPAQEIPFPYMQPGGANANFRYEPGAWNI
                     GVDSLIAGMPDDAAGIKAIRASLKGRLIAWDPITQSEKWSVQYPGPWNGGVLATAGGL
                     VFQGSATGELAAYDAATGRKVWSFDAQTGIVAPPMTYEIDGEQYVAVMAGWGGGFALT
                     IGAGADPRSEGPRRVLVFKIGGKGVLPPRPVSTRAMPDLPPVTGTPAQIAAGKATFAT
                     YCGVCHGSSAVSDFSIPDLRYSPVMTDAATWKGVVIDGDREANGMVSFARFLTPARAE
                     EVRQYVLSLAHGQKAAAAAGK"
BASE COUNT          490 a         1246 c         1242 g          571 t
ORIGIN      
        1 cgacgcccgc gcccgggcct ggctcgacat caactgcggc cattgccaca gccccagggg
       61 accggccgac acttcgggcc tctatctcga cgccgcctcg ccggccgggg cgtcgctggg
      121 tctgtgcaag ccgccggtgg cggcggggca gggcaccggc ggcttctcgc acgacatcga
      181 gccgggccgg cccgatgagt cgatcctgat gttccgcacc ggctcgaccg atccgggggc
      241 gatgatgccg gaactcggcc ggtcgctggc ccacgccgaa ggcctggccc tgctgcgacg
      301 gtggatcgcc gcgatgccct ccggctgtcg ctgatgccgc cgcccggggg tccggatcgc
      361 acctggctgt cgatccagca cctgcgcggc ctggcggctc tgtcggttgc cctgtttcac
      421 gcctgccagt ggtcgcaggt ggacttcgcc acgggccagg cgggcgtcga cgtgttcttc
      481 gtcatcagcg gcttcgtcat gtggaccgcg accagcggcc gggcggtccg gccggccagg
      541 ttcctgttgc gccggctgat ccgggtcgcg ccgctgtact ggctgatcac cttggccctg
      601 gccggcggcg tgctgctgtt tccggcccgg tttcccgatc tcgcggtgac tccggcgcat
      661 ctgctgctgt cgctggcctt cgtgcagcac atgaacccgg cgggccagcc gtttccactg
      721 ctcgcgcccg gctggacgct cgattacgag gccgccttct acctggtgtt tgccgccctg
      781 ctggtccttc cggaaggccg gcggctgttc gccctgaccg tggcgctggt cgttctggcc
      841 ttcgccggct tcctttggcc gccggcctac cagatgcttc tgaacccgct gttcctcgag
      901 ttcctcgccg gcctgtggct gggacggatg gcccagctgc agctgttgcc cgatcggtcc
      961 ctcggctggt tgatgctggg gggcgccgtg ggcctgttta tcctgatcca gctggtcaac
     1021 ctggactggg acctgtggcg gccgatggtc tggggcgttc cggcggccct gctggtggcc
     1081 ggcgcggtga gcatcgaggc ggacggtgga tggaaggctg tcccgggcct gcaaacgctc
     1141 ggcgacgcgt cctattccac ctatctgatc cacaccctga cgatcggcgc cctggccatg
     1201 accatcgggg cctggaaccc gccgctgttt atcccgctgg cgatgatcgt ggcggttgtc
     1261 tccggcgtcg gcctctgggc gctgatcgaa cggccaatgc tcgacgggct gcggcggcgg
     1321 cttgcgtgac gctggggacg gtccgcatac tcgcgccaac gccgcgcggc agacgcggtc
     1381 caagtcggga gctcgggaca tgaggcggat cagcgcgctg gcggtgactc tggcggcggc
     1441 ggccatgctg gcaggctgcg gcaagaaggc ggcggacatc gacggggcga gaatcgccgc
     1501 cgcggacgtc aatcccgccg agtgggtcac ccacggccgg acctacggcg aacagcggtt
     1561 cagcccgctc gacaaggtca atgtcggcaa cgtcggccag ctgggcctgg cctggagcta
     1621 tgacctgccc gagaaccgcg gcgtcgaggc gacgccgctg gtcgccgacg gagtcatgta
     1681 caccacctcg tcctggtcga tcgtccgcgc cttcgatgcg aagaccggca agttgctttg
     1741 ggagttcgac cccaaggtgc cgcgcaagac cggcgtgaag gcctgctgcg acagtgtcaa
     1801 tcgcggcgtc gccctgtggg aggggcgggt ctatttcggc gccctcgacg gtcgcctgat
     1861 cgcgctggac gccaggaccg gcaagcaggc ctggtccgtc gtcacggtcg accagagcaa
     1921 gccctacacc atcacgggcg ctccgcgcgt catcaagggc aaggtgctga tcggcaacgg
     1981 cggcgcggag ctcggcgtgc gcggctacct gtcggcctac gacgcccgca acggcaagat
     2041 gctctggcgc ttcttcaccg tgcccggcga gcaggcgacc gacggcgcgg cctccgacgc
     2101 ggcgatgaag atcgcccggc cgacctggaa gggcgagttc tggaaggtcg gcggcggcgg
     2161 caccgtctgg gattccatgg cctacgaccc cgacctcgac ctgctgtaca tcggcgtcgg
     2221 caacggctcg ccgtggaacc gcgacatccg ctcgcccggc ggcggcgaca acctgttcct
     2281 gtcctcgatc gtcgcgctca agcccgacac cggccagtac gtctggcact accagaccac
     2341 gccgggcgag agctgggact atacggccac ccagcacatc atccttgccg acctgaccat
     2401 cggcgggaag acggtcaagg ccctgatgca ggcgcccaag aacggcttct tctatgtgct
     2461 ggaccggacc aacgggaagc tgatctcggc gaagaactac gtgccgcaga cctgggcgac
     2521 gggcgtcgat ccggcgaccg gccggccgat cgagacgccc aacgcccgct atggctcggg
     2581 cccgtccctg gcgatgccgg ggccgctggg cggccacaac tggcagccga tggcctacag
     2641 ccccaggacc aatctggtct acatcccggc gcaggagatc ccgttcccct acatgcagcc
     2701 cggcggcgcc aacgccaatt tccgctacga gccgggggcc tggaacatcg gtgtcgacag
     2761 tctgatcgcc ggcatgccgg acgacgcggc gggcatcaag gcgatccgcg ccagcctcaa
     2821 gggccggctg atcgcctggg acccgatcac ccagagcgag aagtggagcg tgcagtatcc
     2881 gggcccgtgg aacggcggcg tgctggccac ggcaggcggt ctggtgttcc agggctcggc
     2941 gaccggcgag ctggcggcct atgacgcggc cactggacgg aaggtctggt cgttcgacgc
     3001 ccagaccggc atcgtcgctc cgccgatgac ctacgagatc gacggtgagc aatacgtcgc
     3061 cgtcatggcc ggctggggcg gcggcttcgc cctgaccatc ggcgccggcg ccgatccccg
     3121 ctcggaaggc ccgcgtcggg tgctggtgtt caagatcggc ggcaagggcg tcctgcctcc
     3181 gcgtccggtc tcgacccgcg ccatgcccga cctgcctccg gtcaccggca cgccggcgca
     3241 gatcgcggcg ggcaaggcga ccttcgccac ctattgcggc gtctgtcacg gctccagcgc
     3301 ggtgtccgac ttctccatcc ccgacctgcg ctactcaccg gtgatgaccg atgcggcgac
     3361 ctggaagggc gtggtgatcg acggcgaccg cgaggccaac ggcatggtct ccttcgcccg
     3421 cttcctgacc ccggccaggg ccgaggaggt gcgccagtac gtgctgtcgc tggcgcatgg
     3481 ccagaaggcg gcggccgccg ccgggaaata ggggacgcat gatcgccgcg gagccgccgc
     3541 ccgccgtcg
//
LOCUS       DAORNX010000327         3545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_19866_length_3545_cov_8.448997, whole genome shotgun sequence.
ACCESSION   DAORNX010000327 DAORNX010000000
VERSION     DAORNX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19866_length_3545_cov_8.448997"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="trxA"
                     /locus_tag="PLO65_12870"
     CDS             complement(<1..630)
                     /gene="trxA"
                     /locus_tag="PLO65_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRD29186.1"
                     /translation="MTLIGEGLAPKAAPGDIIKEGSDAGFMADVIEASKTTPVIVDFW
                     ATWCGPCRQLTPALEKAVTAMGGAVKLVKIDVDRNPAYAGQLRVQSIPTVYAFVNGQP
                     VDGFMGALPDSQVKAFIDKLVGPPPVSQIDEVLAQAKESMDLGDMGGAAQGYATVLQM
                     DPANLPAIGGLARCYIAGGDIERAAEVVALAPEGAKDADLDAARAALALA"
     gene            complement(721..1230)
                     /locus_tag="PLO65_12875"
     CDS             complement(721..1230)
                     /locus_tag="PLO65_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRD29187.1"
                     /translation="MKTRAELFAFLDTHGIAHATLDHPAVFRVGEGDEIKAALPGGHT
                     KNLFLKDARGQLWLISALGETVIDLKTLHQVIGSARLSFGNAALMEETLGVTPGSVTA
                     FGLINDSDHRVRFVLDAALARADPVNFHPLTNTATTAVSQAGFRRFLAALGVEPLIVD
                     FTTMRVVEG"
     gene            1317..2060
                     /locus_tag="PLO65_12880"
     CDS             1317..2060
                     /locus_tag="PLO65_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HRD29188.1"
                     /translation="MLLECYPTSQRPPDIVPGRPQRAWMDAFAERHPYRCLPLSMANT
                     SGWEILCPVGFSMEWNGGAHQDCITFAPDHGHPDFHDFAKSHFSRGIVTFHPGYLFRT
                     PPGWSMWAGGPPNHVKDGIQPLAGLVETDWLPFPFTMNWIFTRPGRVRFEKGEPFCFI
                     TMLQDKALAQVQPVTRSLASNDDLRAQYDAWYRQREEFNRRLMKQEPDAVRDAWQRYY
                     FKGELPEQTGPAPKDHVNKRRLKSPRLGV"
     gene            2154..2228
                     /locus_tag="PLO65_12885"
     tRNA            2154..2228
                     /locus_tag="PLO65_12885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2186..2188,aa:Gly,seq:gcc)
     gene            2413..>3545
                     /locus_tag="PLO65_12890"
     CDS             2413..>3545
                     /locus_tag="PLO65_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD29189.1"
                     /translation="MTTTPWATGLQLRSLVTADARLELSLQSTETPAPGEDEVVVRVL
                     ASPINPSDLGLLLGPADIGTARVSENANGPVVTFDIPERLMRMVAARVGESMPVGNEG
                     AGVVVATGTSPQARALLGKTVAVLGGAMYGQYRTLPVASCMALPDGTAPAEGASCFVN
                     PLTALSFPEVMRREGFSALIHTAAASNLGQMLQKICLKDGIGLVNIVRNAEQEAILRD
                     IGATHVVNSSSPTFMDDLTQAIVETGAYLAFDAIGGGKLAGQILTAMEIAANKTAKVY
                     SRYGSTQAKQVYIYGGLDTGPTELNRAFGMMWGLGGWLLTPFLMKIGPVEGQKLRDRV
                     VAELKTTFASHYTRELSLREVLNPEVVAAYNRKATGEKFLINP"
BASE COUNT          562 a         1273 c         1163 g          547 t
ORIGIN      
        1 ggccagggcg agggccgcgc gggcggcgtc gagatcggcg tccttcgccc cctcgggtgc
       61 gagggcgacc acttcggcgg cgcgctcgat gtcgccgccg gcgatgtagc accgggcaag
      121 gccgccgatc gccggcaggt tggccgggtc catctgcagc accgtggcgt agccctgggc
      181 cgccccgccc atgtcgccga ggtccatcga ctccttagcc tgggccagca cctcgtcgat
      241 ctgcgagacg ggcggcggcc cgaccagctt gtcgatgaag gccttcacct ggctgtcggg
      301 cagggccccc atgaagccgt cgaccggctg gccgttgacg aaggcgtaga ccgtcggaat
      361 cgactggacg cgcagctggc cggcgtaggc ggggttcctg tcgacatcga tcttgaccag
      421 cttgaccgcc ccgcccatcg ccgtgacggc cttttccagc gccggcgtca actggcggca
      481 ggggccgcac caggtggccc agaagtcgac gatcaccggc gtggtcttcg aggcctcgat
      541 gacatcggcc atgaagccgg cgtccgagcc ctccttgatg atgtcgccgg gggccgcctt
      601 cggggccagg ccctctccga tcaaggtcat gtcttccgtc ccgtgtgcgg gggcgccgcc
      661 ccgcgttgaa aacgcctgcc cgccaagatg gtcgcagcag ggcttcggtt caattccgca
      721 tcacccctca accacccgca tcgtcgtgaa atcgacgatc agcggctcca cgcccagcgc
      781 cgccaggaag cggcggaagc cggcctgcga cacagctgtg gtcgcggtgt tggtcagggg
      841 gtggaaattg accggatcgg ccctggccag ggccgcgtcg agcacgaacc gcacccggtg
      901 gtcgctgtcg ttgatcagac cgaaggcggt caccgagccc ggcgtcacgc cgagcgtctc
      961 ctccatcagg gcggcgttgc cgaacgacag ccgcgccgag ccgatcacct gatgcagggt
     1021 cttcaggtcg atcaccgtct cccccagcgc cgaaatcagc cacagctggc ccctggcgtc
     1081 cttgaggaac aggttcttgg tgtggccgcc gggcagggcg gccttgatct cgtcgccctc
     1141 gccgacccgg aacaccgccg gatggtcgag ggtcgcatgg gcgatcccgt gggtgtcgag
     1201 aaaggcgaat agctcggctc tggtcttcat gcccccgcca tagccaaacc aaggcgcttg
     1261 cggaaccgcc ctccgcgcgc gacaggatcg ggcgaggacc aatcagcgag gccaccatgc
     1321 tcctggagtg ctacccgacc agccagcgcc cgcccgacat cgtgccgggc cggccgcaac
     1381 gcgcctggat ggacgccttc gccgagcgcc acccctaccg ctgcctgccg ctgtcgatgg
     1441 ccaacacctc gggctgggag atcctctgcc cggtcggctt ttccatggaa tggaacggcg
     1501 gggcgcacca ggactgcatc accttcgccc cggaccacgg ccacccggac ttccacgact
     1561 tcgccaagag tcacttttcg cgcggcatcg tcaccttcca cccgggctac ctgttccgca
     1621 cgccgccggg ctggtcgatg tgggccggcg gtccgcccaa ccacgtcaag gacggcatcc
     1681 agccgctggc cggcctggtc gagaccgact ggctgccctt ccccttcacc atgaactgga
     1741 tcttcacccg ccccggccgc gtgcggttcg agaagggcga gccgttctgc ttcatcacca
     1801 tgctgcagga caaggccctg gcccaggtcc agccggtgac ccgctcgctg gcctcgaacg
     1861 acgacctgcg ggcgcagtac gacgcctggt atcgccagcg cgaggagttc aaccgccgcc
     1921 tgatgaagca ggagcccgat gccgtgcgcg acgcctggca gcgctactat ttcaagggcg
     1981 agctgcctga gcagaccgga ccggcgccga aggaccacgt caacaagcgg cggctgaagt
     2041 cgccccgcct cggggtctga tttcgacgcc gggagacgct tgcaaaggcc ctgaccctct
     2101 gccataagcc gcctctcgat tttcgggacg ccctccaaaa gcgtccctgg agcgcgggcg
     2161 tagctcagtg gtagagcaca accttgccaa ggttggggtc gagagttcga atctcttcgc
     2221 ccgctccatt cttccgcaat agacctgacc aggacggccc cgcgaggggc cgttttgcat
     2281 ttcgcggccg gcggtgggcg tcccgttggc ctcctctccc cgtcccttcc ggaacagtcc
     2341 tcttcccccg gctcccccga tgcgcttccc tgacaacgaa tccggatgag ccggaagcgg
     2401 gagcgcgacg ccatgaccac caccccctgg gccaccggcc tgcaactgcg gtccctggtg
     2461 accgccgacg cccggctcga gctgtcgctg cagtcgaccg agacgccggc gccgggcgag
     2521 gacgaggtgg tggtccgggt cctggcctcg cccatcaacc cgtcggacct gggcctgctg
     2581 ctcggcccgg ccgacatcgg aacggcgcgg gtgagcgaga acgccaacgg gccggtggtc
     2641 actttcgaca ttcccgagcg cctgatgcgg atggtcgccg cccgggtcgg cgagagcatg
     2701 ccggtcggca acgagggcgc gggcgtcgtc gtggcgaccg ggacctcgcc gcaggcgcgg
     2761 gccctgctcg gcaagaccgt cgcggtgctt ggcggggcga tgtacggcca gtaccgcacc
     2821 ctgcccgtgg ccagctgcat ggcgctgccc gacgggaccg ccccggccga aggcgcctcc
     2881 tgcttcgtca atccgctgac ggcgctgagc tttccggagg tcatgcgccg ggaggggttc
     2941 tcggccctga tccacacggc ggcggcctcg aacctcggac agatgctgca gaagatctgc
     3001 ctcaaggacg gcatcggcct ggtcaacatt gtgcgcaacg ccgagcagga agcgatcctg
     3061 cgcgacatcg gcgccaccca tgtggtcaat tcgtcctcgc cgaccttcat ggacgacctg
     3121 acccaggcca tcgtcgagac gggcgcctac ctggccttcg acgccatcgg cggcggcaag
     3181 ctggccggcc agatcctcac cgccatggag atcgccgcca acaagacggc caaggtctac
     3241 agccgctacg gctcgaccca ggccaagcag gtctacatct acggcgggct ggacaccggc
     3301 ccgaccgaac tgaaccgcgc gttcggcatg atgtggggtc tcggcgggtg gctgctgacg
     3361 ccgttcctga tgaagatcgg tcccgtcgag ggccagaagc tgcgcgaccg ggtggtcgcc
     3421 gaactgaaaa ccaccttcgc cagccactac acccgggaac tgtcgcttcg ggaggtgctg
     3481 aaccccgagg tcgtcgccgc ctataaccgc aaggcgaccg gggagaagtt cctcatcaat
     3541 cccga
//
LOCUS       DAORNX010000328         3521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20056_length_3521_cov_7.185516, whole genome shotgun sequence.
ACCESSION   DAORNX010000328 DAORNX010000000
VERSION     DAORNX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_3521_cov_7.185516"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLO65_12895"
     CDS             complement(<1..252)
                     /locus_tag="PLO65_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HRD29190.1"
                     /translation="MADLARLTGARLEPSDNPDALIVGAAPLDRAGPADVAFLGDRKH
                     LAALRATRAGACFIGQAHVGDLPAGCVGLVANRPQAAWAA"
     gene            complement(332..988)
                     /locus_tag="PLO65_12900"
     CDS             complement(332..988)
                     /locus_tag="PLO65_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRD29191.1"
                     /translation="MRFNKTFTVAAGAAAALAFASAVAAQTPPPRPATPAPAAPAIKH
                     GPALPGVCTYNGDQAVQDSEVGKYVAARMKQIIAEVNAELTAEDTAIKNEAKAIDAAR
                     AAGPTDALEARAANLQVRFNAFKRKQQLRQAEVQQTERKALMRVLQELDPIAVGVYQA
                     RNCSILLGDGVLLGNPAMDITGQVVTGLNAKIKTFTFNRERMDTPPAGAPAATPPRAN
                     "
     gene            complement(994..3381)
                     /gene="bamA"
                     /locus_tag="PLO65_12905"
     CDS             complement(994..3381)
                     /gene="bamA"
                     /locus_tag="PLO65_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRD29192.1"
                     /translation="MKHARAHSAAIVTLAALLLGTTALATPTVASAQVETGVVQRILV
                     QGNERIEAGTVLSYLPIQAGDTVDSARIDVALKTLFRTDLFADVKIDLQPNGDLVVRV
                     VENPIINRVIFEGNSALKEEKLRDEVTIRPRGIFTRAKVQSDVQRLIELYRKSGRIGV
                     TVTPKVVELPQKRIDLIFEINEGPKSGVLDINILGNVAFSDSDLADVIVTEESHWYKF
                     LSSNDNYDPDRIEYDKEQLRKFYRNKGYYDFRIISAVAELSPERNGFVVTYTIEEGEK
                     YKFGKLTVETELKKLDGAVLQQLLPIRTGQVYQDELIEQATDSLTFAAGAAGFAFVDV
                     RPRYIPNRETHTVDVVFQVKEGPRVYIDRIDIVGNTRTLDYVIRREMNVSEGDAYNRV
                     LVDRSKQQIRALGFFKDVNVEEVPSGVPDRTTLRVTVEEQPTGELSFSAGYSSVDQLI
                     LDLGVSERNFRGRGQNVRARISVGSLRQQIDFSFTEPRFLGRDLRAGVDVYSYRYDFS
                     QQTAFDTRSTGAGLRLGFPLTTNASMGLRYTLRTDDVVVSDSYCIPGQEVVSPTLCAQ
                     RGSNVTSLIGYTVRLDRRNDVLKPTRGFFVELNQDIAGVGGDVNYLRTESNGGWYYGF
                     NKDFVLSATGSFGYIDGWHGDDVRINDRFYKGGNSFRGFEVAGIGPRDTSFGRNDALG
                     GKLYAIGSLELSVPTFLPEQYGIKASVFTDVGTLGLLDDADQRDRFGQIIDCDTSSTT
                     VTPCIKDDLGLRASAGLSIFWRSPMGPIRFDFSQILAKEDYDKTETFRFSTSTRF"
BASE COUNT          582 a         1102 c         1225 g          612 t
ORIGIN      
        1 cgcggcccag gccgcctggg gccggttcgc gaccagcccg acgcagccgg ccggcaggtc
       61 gccgacatga gcctggccga tgaaacaggc cccggcgcgc gtggccctga gcgccgccag
      121 atgcttccgg tcgccgagga acgccacatc ggccggcccc gccctgtcga gcggcgccgc
      181 gccgacgatc agcgcgtcag gattgtccga cggttccagc cgggcgccgg tcagtcgggc
      241 gaggtccgcc aatggaaggg ggcctcgact gaggtagaat cgcgggtctg gcatcacgtc
      301 ctcgcacaga cccgcgatag cgcgagtcgg actagtttgc gcgcggcggc gtcgctgccg
      361 gggccccggc cggcggagtg tccatgcgct cacggttgaa ggtgaacgtc ttgatcttgg
      421 cgttcaggcc cgtgaccacc tggccggtga tgtccatcgc cggattgccg agcaacacgc
      481 cgtcgcccag caggatcgaa cagttgcgcg cctggtagac gcccaccgcg atcgggtcca
      541 gttcctgcag aacgcgcatc agcgccttgc gttcggtctg ctggacctcc gcctggcgca
      601 actgctgctt gcgcttgaag gcattgaacc ggacctgcag gttggccgcg cgggcctcca
      661 gcgcgtcggt cggaccggcg gcgcgggcgg cgtcgatggc cttggcttcg ttcttgatcg
      721 ccgtgtcctc ggcggtcagt tcggcattca cctcggcgat gatctgcttc atacgggcgg
      781 cgacgtactt gcccacttcc gagtcctgga cggcctggtc accgttgtag gtgcagactc
      841 cgggaagggc cggaccatgc ttgatcgccg gcgccgcggg cgcaggcgtg gccggacgcg
      901 gcggtggcgt ctgggccgcg acggccgagg cgaaggcgag agcggcggcc gcgccggccg
      961 cgaccgtgaa ggtcttgttg aatcgcatgg agtctagaac cttgtggagg tggagaaacg
     1021 gaacgtttcc gtcttgtcgt agtcttcttt ggccagaatt tggctgaagt cgaaacgaat
     1081 tggccccatc ggcgaccgcc agaagatcga aagacccgcc gaggcgcgca aaccgaggtc
     1141 atccttgatg cacggcgtga ccgtggtgga cgaggtatcg cagtcgatga tctggccgaa
     1201 gcggtcgcgc tggtcggcgt cgtccagaag gccgagcgtg ccgacgtccg tgaacaccga
     1261 cgccttgatg ccgtactgct cgggcaggaa ggtcggcacc gacagctcca gggagccgat
     1321 ggcgtagagc ttgccgccca gggcgtcgtt gcggccgaac gaggtgtcgc gcggaccaat
     1381 gccggcgacc tcgaagccac ggaaggagtt tccgcccttg tagaagcggt cgttgatgcg
     1441 gacgtcgtcg ccgtgccagc cgtcgatgta gccgaacgag ccggtcgcgc tcagcacgaa
     1501 gtccttgttg aagccgtagt accagccccc gttcgactcg gtccgcaggt agttcacgtc
     1561 gccgccgacg ccggcgatgt cctggttcag ttcgacgaag aagccgcggg tcggcttgag
     1621 cacgtcattg cgccggtcca gccgcaccgt gtagccgatc agcgaggtga cgttggagcc
     1681 ccgctgggcg cagagggtcg gggacaccac ctcctggccg gggatgcagt aggaatcgga
     1741 caccaccacg tcgtcggtgc gcagggtgta gcgcagcccc atcgaggcgt tggtggtcag
     1801 cgggaagccc aggcgcaggc cggcgccggt cgagcgggtg tcgaacgccg tctgctggct
     1861 gaagtcgtag cggtaggaat agacatccac gccggcccgc aggtcgcggc cgaggaagcg
     1921 cggctccgtg aacgagaagt cgatctgctg gcgcagcgaa ccgacggaga tgcgggcgcg
     1981 gacgttctgg ccccgtccgc ggaagttgcg ctcgctgacg cccagatcga ggatcagctg
     2041 gtcgaccgag ctgtagccgg cgctgaaaga cagttcgccc gtcggctgct cctcgaccgt
     2101 cacgcgcagg gtcgtgcgat cggggacccc gctcgggacc tcctcgacgt tgacgtcctt
     2161 gaagaagccg agggcgcgga tctgctgctt tgaccggtca accagcacgc ggttgtaggc
     2221 gtcgccctcg gagacgttca tctcgcggcg gatcacatag tcgagcgtgc gggtgttgcc
     2281 gacgatgtcg atgcggtcga tgtagacccg cgggccttcc ttgacctgga agaccacgtc
     2341 gacggtgtgg gtctcgcggt tgggaatgta gcgcgggcgg acgtcgacga aggcgaaccc
     2401 ggcggcgccg gccgcgaagg tcagcgaatc ggtggcctgc tcgatcagtt cgtcctggta
     2461 gacctggccc gtgcggatcg gcaggagctg ctgcagcaca gcgccatcga gcttcttcag
     2521 ctccgtctcg accgtcagct tgccgaactt gtacttctcg ccttcctcga tcgtgtaggt
     2581 gaccacgaag ccgttgcgct cgggcgacag ctcggcgacg gccgagatga tccggaagtc
     2641 ataatagccc ttgttgcggt agaatttccg caactgttcc ttgtcgtact cgatccggtc
     2701 cggatcgtag ttgtcgttgc tcgagaggaa cttgtaccag tgggactcct cggtgacgat
     2761 cacatcggcc aggtcgctgt ccgaaaaggc cacgttgccg aggatgttga tgtcgaggac
     2821 gccgctcttg gggccttcgt tgatctcgaa gatcaggtcg atgcgcttct gcggcagctc
     2881 gacgaccttg ggggtcaccg tcacgccgat gcggcctgac ttgcgataga gctcgatcag
     2941 gcgctggacg tccgactgga ccttggcgcg ggtaaagatg ccccgcggcc ggatggtcac
     3001 ctcgtcgcgc agcttctctt ccttgagcgc cgagttccct tcgaagatca cccggttgat
     3061 gatcgggttc tccaccacgc gaacgaccag gtcgccgttc ggctgcaggt cgatcttgac
     3121 gtcggcgaac aggtcggtgc ggaacagggt cttgagcgcc acgtcgatgc gcgcggaatc
     3181 gaccgtgtcg ccggcctgga tcggcaggta ggacagcacc gtcccggcct cgatccgctc
     3241 gttgccctgg accaggatgc gctggaccac ccccgtttcg acctgggcag aggccacggt
     3301 cggggtggcc agcgccgtcg tccccagaag gagcgcagca agggtaacaa tcgcagcgct
     3361 gtgcgcacga gcatgcttca taaggtctgc catcggcggt cggcgggtgc tcatgagaac
     3421 agaccaccga ggaagttgaa cacgttgtag cgctgcaggt cgttccagac cgcgaacagc
     3481 atgaatccca acagcaaggc aagaccaagg cgatagccga c
//
LOCUS       DAORNX010000329         3517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20096_length_3517_cov_4.759099, whole genome shotgun sequence.
ACCESSION   DAORNX010000329 DAORNX010000000
VERSION     DAORNX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20096_length_3517_cov_4.759099"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>313)
                     /locus_tag="PLO65_12910"
     CDS             complement(<1..>313)
                     /locus_tag="PLO65_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD29193.1"
                     /translation="LRQAIGLQQSPPGMLLTTLALFLTYFVMEPVFTAAWAAGMVPLE
                     LGSDAGGSIRVPASFCGVFGHLSSFDIISLEGFGPPGGGMIAQGRNWAAAGPMARRAE
                     DL"
     assembly_gap    314..483
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<484..692)
                     /locus_tag="PLO65_12915"
     CDS             complement(<484..692)
                     /locus_tag="PLO65_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29194.1"
                     /translation="MQASGDWLATAGAGEVAAAVRARSVSAVEAVEAAIARIEARDGA
                     INAVVVRDFDRALDQARRVDRAIAAG"
     gene            844..1128
                     /locus_tag="PLO65_12920"
     CDS             844..1128
                     /locus_tag="PLO65_12920"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRD29195.1"
                     /translation="MAHPKIGAAAATARLNGWTAVAGRDAIHKTFTFADFNAAFGFMS
                     RVALMADKLDHHPEWSNVYNRVEVLLATHDADGVTELDVTLAGFMDEIAG"
     gene            1297..2625
                     /locus_tag="PLO65_12925"
     CDS             1297..2625
                     /locus_tag="PLO65_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29196.1"
                     /translation="MSLWRLLAFATPTIPLAAMLMPVTAYLPNYYAQDLKVDLAELGW
                     AFMVVRFFDLWFDPALGLVMDRTRSRWGRFRPWFVVGAPIAMLATYMLFMARPGIGGG
                     YILLWLIVGFGGQSMSQLGHMAWAATVAPGYDERSRVYGWWQAMTVSGMIAILLMPPL
                     VRFGFGGDFSTGVKAMGWFTIIALPLTTVLALSATGEPAARPHHETPRLGHILAMLKR
                     GSVLRVLAADICWGTALSVSGTLLFFFFDAVRGVERGMAGLMLIVFFVGALAGAPIWR
                     VVARRLGKHRAMMIAGVCWAVMQMSVFVAPNLLWVGFLTMFLAGLPYASGPIMLKAMM
                     ADVADEERLESGVDRTGLLFSLLTGSVKIGSMLAVGGSLFLLKEVGFVAERSGDNSAT
                     ALLTLQLMFCLGPAALALAAAWLISGYRLDATAHALIRRKLDARDEATLV"
     gene            complement(2716..3189)
                     /locus_tag="PLO65_12930"
     CDS             complement(2716..3189)
                     /locus_tag="PLO65_12930"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRD29197.1"
                     /translation="MKKLIAAAVALSTAAFVALPAAAYVYPTGGVSAQEVSSVLRAKG
                     LPVEITTDDAGDPMIKSSSNNLNWRVYFYDCKAGRCTSIQFSSGFDLDNGMTYAKCNE
                     WNYTKRFSRCALDDEMDPYTRYDIDVAKGYTSESLAYALDTWLLVVPTFSSFIGY"
BASE COUNT          487 a         1123 c         1184 g          553 t
ORIGIN      
        1 aaggtcctcc gccctgcgcg ccatcggccc ggccgcggcc cagttgcggc cctgggcgat
       61 catgccgccg ccaggcgggc cgaagccctc gagggagatg atgtcgaacg acgagaggtg
      121 gccgaacacg ccgcagaacg acgccgggac acggatcgag ccgccggcgt cgctgccgag
      181 ctcgagcggg accatgccgg ccgcccaggc cgcggtgaac accggctcca tcacgaaata
      241 ggtcaggaac agcgccagcg tggtcagcag catgccgggc ggcgattgct gcagcccgat
      301 ggcctggcgc aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnncccgccg cgatagcccg gtcgacccgc cgcgcctggt ccagcgcgcg gtcgaagtcg
      541 cgcaccacca ccgcgttgat cgccccgtcg cgcgcctcga tccgcgcgat cgccgcctcg
      601 accgcttcca cggcgctgac cgatcgcgcg cgaaccgccg ccgccacctc cccggcgccc
      661 gcggtcgcga gccagtcccc tgaagcctgc atgatggtga gccctttcca ttgagggctt
      721 cgaagtctgc gcataaaaac cggcgggcca atgcttgatc tcgactgttt tatgcccagt
      781 tcaggttggg atggcccgcc cgcggccgtg ctatagccgc cccaagcttc acggagacga
      841 gtcatggccc atccgaagat cggcgcggcg gcggcgacag cgcggctcaa cggctggacg
      901 gcggtggccg ggcgcgacgc catccacaag accttcacct tcgccgattt caacgcggcc
      961 tttggcttca tgagccgcgt ggcgctgatg gccgacaagc tcgaccacca tcctgaatgg
     1021 tccaacgtct acaaccgggt cgaggtgctg ctggcgaccc atgacgccga cggcgtcacc
     1081 gagctggacg tgaccctggc cggcttcatg gacgagatcg ccggataggg cagggcgatc
     1141 cccttgcgcg acgggcgata gcgacggaac atcggcgaac caccccgcga agagggggac
     1201 ctcgtggggg ggatcagagt gtcagtggag ctggacccgg ccgtcacggg cccgtcgacg
     1261 acgcagggga gacccgcggg ggcaggcccg gcccggatgt ccctctggcg gctgctggcc
     1321 ttcgccaccc cgaccattcc cctggccgcc atgttgatgc cggtcacggc ctacctgccc
     1381 aactattacg cccaggacct gaaggttgat ctggccgagc tcggctgggc cttcatggtg
     1441 gttcggttct tcgacctgtg gttcgacccg gccctcgggc tggtgatgga ccggacgcgc
     1501 tcgcgctggg gccgtttccg gccgtggttc gtcgtcggcg ccccgatcgc catgctggcg
     1561 acctacatgc tgttcatggc ccggcccggc atcggcggcg gctacatcct gctgtggctg
     1621 atcgtcggct tcggcggcca gtcgatgtcg cagctggggc acatggcctg ggcggcgacc
     1681 gtggcgccgg gctacgacga gcggtcgcgc gtctatggct ggtggcaggc gatgaccgtc
     1741 agcgggatga tcgccatcct gctgatgccg ccgctggtca ggttcggctt cggcggcgac
     1801 ttctccaccg gggtcaaggc gatgggctgg ttcaccatca tcgccctgcc gctgaccacc
     1861 gtgctggccc tgtcggccac cggcgagccg gcggccaggc cgcatcatga aacaccgagg
     1921 ctgggccata tcctggccat gctgaagcgg ggctcggtgc tgcgggtgct ggcggccgac
     1981 atctgctggg gcacggccct gtcggtctcg ggcaccctgc tgttcttctt cttcgacgcc
     2041 gtgcgcgggg tcgagcgggg catggcgggg ctgatgctga tcgtgttctt cgtcggggcc
     2101 ctggccgggg cgccgatctg gcgggtcgtc gcccgccgcc tcggcaagca tcgcgcgatg
     2161 atgatcgcgg gcgtctgctg ggcggtgatg cagatgtcgg tgttcgtcgc gccgaacctg
     2221 ctgtgggtcg gctttctgac catgttcctg gccggtctgc cctatgcctc gggcccgatc
     2281 atgctcaagg cgatgatggc cgacgtggcc gacgaggaac ggctggagag cggcgtcgat
     2341 cgcaccggcc tgctgttctc gctgctgacg ggctcggtca agatcggctc gatgctggcg
     2401 gtcggcggca gcctgttcct gctcaaggaa gtcggcttcg tcgccgagcg gtcgggcgac
     2461 aacagcgcga ccgccctgct gacacttcag ctgatgttct gcctcggccc ggcggccctg
     2521 gccctggcgg cggcctggct gatcagcggc taccggctgg acgcgacggc ccacgccctg
     2581 atccggcgca aactcgacgc ccgggacgag gcgaccctgg tctgagcccg acagacagac
     2641 gcccggcgcg ccatgacggc ggccgggcgt ccgggatcgt cagcggtgcg acgcgcgagg
     2701 cgccgccccc ctcggtcagt agccgatgaa gctcgagaag gtcggcacca ccagcagcca
     2761 ggtgtcgagg gcgtaggcca gcgactcgga ggtgtagccc ttggccacgt cgatgtcgta
     2821 gcgggtgtag gggtccatct cgtcgtccag cgcgcagcgg ctgaagcgct tggtgtagtt
     2881 ccactcgttg cacttggcgt aggtcatgcc gttgtcgagg tcgaagcccg acgagaactg
     2941 gatgctggtg cagcggcccg ccttgcagtc gtagaagtag acgcgccagt tcaggttgtt
     3001 cgacgagctc ttgatcatcg gatcaccggc gtcgtcggtg gtgatctcga cgggcaggcc
     3061 cttggctcgc aggacactgg acacttcctg ggcgctgacg ccgccggtcg gatagacata
     3121 ggcggccgcg ggaagcgcga cgaaggcggc cgtgctgagc gcgacggcgg cggcgatgag
     3181 ctttttcatg agtgaactcc cccaagtgat actggtgatt accaccctat ggcccgtcct
     3241 gtcgccggcg ccatgcccca aaagaggggg ttggggcggg gagaggggcc gggggcgagg
     3301 cgtcagtagc taggggctgg atggccaggc acgccatggg gcgccattca cgctaaggcc
     3361 aatcgacaat cacgacctgg agccgtcttt tcgccgatcg tccccgcgaa ggcggggacc
     3421 caagctcttg tggctgccga aacggcgcgt caggatccga tacaccgctt gggttcccgc
     3481 ctccgcggga accatcggag cccctggcgg ctctcgc
//
LOCUS       DAORNX010000330         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20168_length_3508_cov_4.866203, whole genome shotgun sequence.
ACCESSION   DAORNX010000330 DAORNX010000000
VERSION     DAORNX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20168_length_3508_cov_4.866203"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLO65_12935"
     CDS             <1..364
                     /locus_tag="PLO65_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29198.1"
                     /translation="RIVNISSIHERAPRAGAADYDAAKGGLAQLTRTLALELAPGRIA
                     VNGVAPGMILSPMNARAVADADYRAALESHIPWGRAGRANEVARLVLFLLSPDADYIT
                     GATVVIDGALSLTVAQGA"
     gene            369..1331
                     /locus_tag="PLO65_12940"
     CDS             369..1331
                     /locus_tag="PLO65_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29199.1"
                     /translation="MTTYSIEAIEEVTLAEGSPLEGMDLMSPFVWREGERYRMLVRRV
                     PRPLRDTDPTGIICSGASHDGLCFEMDLKPAILPGPESFDAGGCEDPTVLVLDDGGYQ
                     VFYTGVDAARRQGSLLVAEGPDLIHLVKRRVMLKAPPGQGNIKEATLARTASGDWRLF
                     YEYAAHDASRIGVASGPTSKGPWTLLPNPFGIRQDSWDNWHLSTGPIAMVEGEDPVMF
                     YNGATVDARWRIGWITFDPDFTRVTGRGLEPLLLPPPAKDRAATDIAFAASTVVEGGL
                     ITLYYSLEDATLRRARVGRYAAIEPAQKVSPSNSAAAATGDAPT"
     gene            complement(1332..1673)
                     /locus_tag="PLO65_12945"
     CDS             complement(1332..1673)
                     /locus_tag="PLO65_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRD29200.1"
                     /translation="MARRDITGAVDFDYLERFAAGDQQVVDEVLELFQEQAAMWSPML
                     ESTNDGWRDAVHTVKGAARGVGAHALGEACERAEIEGPQALPAVHGALDAALFDIAAW
                     RHEKALRSLKG"
     gene            1758..1833
                     /locus_tag="PLO65_12950"
     tRNA            1758..1833
                     /locus_tag="PLO65_12950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Lys,seq:ctt)
     gene            complement(1935..>3148)
                     /locus_tag="PLO65_12955"
     CDS             complement(1935..>3148)
                     /locus_tag="PLO65_12955"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD29201.1"
                     /translation="RIRLDAATFLQEDAGPAATDFRRGSFNNSLENDRARDLKDGVTV
                     RSARVMVDGVILRRLEYRAEYDLGDPGLDDGVRLSAAWLQYRLTPTWRGRVGAFAVPT
                     GLEDAAGAGGQLFLERPSSAEVARSVVAGNGRQAVSLQGAGEAWTLSLAATGDRIDGA
                     TTTGQQALLMRATRRIERPGDLVLHLGISGAAVVSPGRSRTGGAPLRLRDRPEIRVDG
                     ARLVDTGDIDVEGYRSVGLEAGLRRGAGIVEAEYFGMRMARTGGAAEAEFSGWYIQGG
                     WILTGESRPYAAATGSFDRPRPVRTFAPRRGDWGALELALRISRLDLDWGGKSAAPIH
                     GGVQTIGSVGLNWYPDDPVLVRLAYSAVRADRRSPGGDAFAGPYPATPPKGAQVGQAL
                     QTLSLMVQIAF"
     assembly_gap    3149..3313
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3314..3494)
                     /locus_tag="PLO65_12960"
     CDS             complement(<3314..3494)
                     /locus_tag="PLO65_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HRD29202.1"
                     /translation="MTRSSLDYLHVSDGWSIGTGPSVVFIDEGFARTLNTTTLTQDVY
                     AIAFGQRGLMAGTGLE"
BASE COUNT          503 a         1241 c         1105 g          494 t
ORIGIN      
        1 ccggatcgtc aacatctcct cgatccacga gcgggcgccg cgagccggcg ccgctgacta
       61 cgacgcggcc aagggcgggc tggcgcagtt gaccagaacc ctcgctctcg agctcgcgcc
      121 agggcggatc gcggtcaacg gcgtcgcgcc cggcatgatc ctcagcccga tgaacgcgcg
      181 ggcggtggcc gacgccgact atcgcgcggc gctcgagagc cacattccct ggggccgcgc
      241 cgggcgggcc aatgaagtcg cccggctggt gctgttcctg ctctcgccgg acgccgacta
      301 catcacggga gccacggtgg tgatcgacgg cgccctgtcg ctgacggtcg cgcaaggagc
      361 gtgaacgcat gaccacctac agcatcgagg cgatcgaaga ggtgaccctc gccgaaggct
      421 cgccgctcga gggcatggac ctgatgagcc cgttcgtctg gcgggagggc gagcggtacc
      481 ggatgctcgt gcgccgggtc cctcgcccgc tgcgtgacac ggacccgacg ggcatcatct
      541 gcagcggcgc cagccatgac ggcctgtgct tcgagatgga cctcaagccg gccatcctgc
      601 cgggccccga gtcgttcgac gccggcggct gcgaggatcc gaccgtcctg gtcctggacg
      661 acggcggcta ccaggtcttc tacacgggcg tcgacgcggc gcgccggcag ggctcgctgc
      721 tggtcgccga ggggcccgac ctgatccatc tggtcaagcg tcgggtcatg ctcaaggcgc
      781 cgcccggaca gggcaacatc aaggaagcca ccctggcccg gacggcgtcg ggcgactggc
      841 ggctgttcta tgaatacgcg gcccacgacg cctcgcgcat cggcgtggcc agcggaccga
      901 cctccaaggg cccctggacg ctgctgccca accctttcgg catccgccag gacagctggg
      961 acaactggca cctgtcgacc ggcccgatcg ccatggtcga gggggaagac ccggtgatgt
     1021 tctacaatgg cgcgacggtc gacgcccgct ggcgcatcgg ctggatcacc ttcgaccccg
     1081 acttcacgcg cgtgacgggc cggggcctgg agccgctact gctgccgccg cccgcgaagg
     1141 accgggccgc caccgacatc gccttcgcgg cctcgacggt cgtcgagggc gggctgatca
     1201 ccctctacta ttcgcttgag gacgccaccc tgcgccgcgc gcgcgtcggt cgctacgccg
     1261 cgatcgaacc ggcgcaaaag gtctcgccca gcaactcggc cgccgcggct acgggcgatg
     1321 cgcccacatg atcagccctt cagcgaccgc agcgccttct cgtgccgcca ggcggcgatg
     1381 tcgaacagcg ccgcgtccag cgccccgtgc acggccggca aggcctgcgg cccctcgatc
     1441 tcggcccgct cgcaggcctc gcccagggcg tgggcgccca caccgcgcgc cgcgcccttg
     1501 accgtgtgca ccgcgtcgcg ccagccatcg ttcgtcgact cgagcatcgg cgaccacatc
     1561 gcggcctgct cctggaacag ttcgagcacc tcgtcgacaa cctgctgatc gccggccgcg
     1621 aaccgttcca gatagtcgaa atcgaccgct cccgtgatgt ctcgtctggc catgcgtatc
     1681 gcccttgcgt ccccggtctt ttcggctatg ttccgcgccc tcgccgccgg gcctcgcaaa
     1741 gccccgaccg tcgatacggg tgcatagctc agttggtaga gcagctgact cttaatcagc
     1801 gggtcgtagg ttcgaatcct actgcaccca ccattgtttt ccccagaaaa ccctggtgtc
     1861 cccggcgggt ctgaagccgc cgcgcggcgg ctcgtcccgc ttccgcccct ggcgatcgat
     1921 tcggccggga cgacctagaa ggcgatctgg accatcaacg aaagggtctg caacgcctgg
     1981 ccgacctggg cgcccttggg tggcgtcgcc gggtagggtc cggcgaaggc gtcgccgccg
     2041 ggcgagcgac ggtccgcgcg gaccgcgctg taggccaggc ggaccagcac cggatcgtcg
     2101 gggtaccagt tgaggccgac cgacccgatc gtctggacac ctccatggat cggcgcggcc
     2161 gactttccac cccagtccag gtcaaggcgg gaaattctca gcgccagttc gagcgcgccc
     2221 cagtcgccgc ggcggggcgc gaaggtgcgg acaggtcgtg gtcggtcaaa actcccggtc
     2281 gcggcggcat agggacggga ctcgcccgtc aggatccagc cgccctgtat gtaccagccc
     2341 gaaaactcag cctcggccgc tccgccggtc ctggccattc gcatgccgaa atactcggct
     2401 tcgacgatcc ctgcgccccg tcggaggcca gcttccagac ccacggaacg atagccctcg
     2461 acgtcgatgt cgcccgtatc gaccagccgg gcgccgtcga cccggatttc cggacggtcc
     2521 cgcagtcgca gcggcgcgcc gccggtcctg ctccgtccgg ggctcacgac cgccgcgccg
     2581 ctgattccca ggtgcagcac caggtcgccc ggtcgttcaa ttcgccgggt ggctcgcatc
     2641 agcagggcct gctgaccggt cgtcgtcgcc ccgtcgatgc gatcgccggt cgcggccagg
     2701 ctcaaggtcc aggcttcgcc cgccccctgc agagacaccg cctgccggcc gttgccggcc
     2761 acgacgctcc gcgccacctc ggccgacgat ggacgctcca ggaagagctg gccgccggcc
     2821 ccggccgcgt cctccaggcc ggtcgggacc gcgaaggccc ccacccgccc ccgccaggtc
     2881 ggagtcagcc gatactgcag ccaggccgcc gaaagtcgga cgccgtcatc caggcccgga
     2941 tcgccgaggt cgtactccgc gcgatactcg agcctgcgca ggatcacgcc gtcgaccatc
     3001 acccgggcgc tccggaccgt cacgccgtcc ttgaggtccc tggcgcggtc attctcaagg
     3061 ctgttgttga acgagccacg gcggaagtcc gtggcggccg ggccagcgtc ctcctgcagg
     3121 aaggtcgcgg cgtcgagccg gatccggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnncctccag cccggtgccg gccatcagcc cccgctgccc gaaggcgatg
     3361 gcgtagacgt cctgggtaag ggttgtcgtg ttcagtgtcc gggcgaagcc ctcgtcgatg
     3421 aacacgacgc tcggccccgt gccgatcgac cacccgtcac tgacatgcag gtagtcgagc
     3481 gacgaccggg tcatgaagaa gagcgcgt
//
LOCUS       DAORNX010000331         3496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20262_length_3496_cov_5.765185, whole genome shotgun sequence.
ACCESSION   DAORNX010000331 DAORNX010000000
VERSION     DAORNX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20262_length_3496_cov_5.765185"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2015
                     /locus_tag="PLO65_12965"
     CDS             <1..2015
                     /locus_tag="PLO65_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD29203.1"
                     /translation="SLTDAGLPVQALQVIRSGLRDAGRSDDAAALKLGLADAGAYASR
                     ELGDLPAFIDNIDIGIAATLATDQPIDGRTALYNLTVLSSSQGRNALAERLLQQYRRL
                     ADETGNGIEIGWANELCAKIKSANRRYAAALQCAAAALTNPDIAAEHRPKVMLLEVIA
                     LARLGRPAEARRQLQALTDLARERGDPVLMHDLLQGEAEVLRSEGRLAEAYEALMRFH
                     DQQRSDMAASVVEGLHGMRASLESEIDDTQALLDAQRRQTLQISVLVGVVALALIAAV
                     VSLLNQRRLQGRLVAAAERAERADRVKSEFLANMSHEIRTPLTSIVGYSRLLADQPDL
                     SPDSAQFAGRIVTATQSLLAIVNDVLDFSKIEAGQARIEPRPTALAGLVQDVAGLFGA
                     QAANKGVTLTVTLAEDLPAWTLVDPDRVRQVLLNLVGNAVKFTAAGEVRVSAAWSSDD
                     GLRIDVVDSGPGISAEGQARLFQRFSQVHGASNRMESGTGLGLAICYGLVTAMGGEIG
                     VESQPGEGSRFWFRTPAPLSDAPATAEMPELVADSPSDRARVLVVDDNEANRELVSHL
                     LRPLDLDLSFATGGEEAVQTAMVDVFDLILMDIRMPGMGGEEAMGRIRSGAGPNAEAP
                     ILAFTADVDGQNTERLLGAGFDGHVHKPIDARGLITSVVQWTARAA"
     gene            complement(2012..2296)
                     /locus_tag="PLO65_12970"
     CDS             complement(2012..2296)
                     /locus_tag="PLO65_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29204.1"
                     /translation="MMFAARLREPVMRGEVTCSVRIWQSPRVKAGGRYALGPGHVVVR
                     SIHEISLADVTPDLARRSGFSGLVDLLKVARHGPGERVFLVEFDYEGPSA"
     gene            complement(2293..3384)
                     /locus_tag="PLO65_12975"
     CDS             complement(2293..3384)
                     /locus_tag="PLO65_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD29205.1"
                     /translation="MIRSTFTRLATLAIGLSMVLAAPALAAPVVTEGDVVVRNFRFQS
                     GETLPDLRLHYRTLGEPRRDASGRVVNAVMILHGTGGSGAQFLRPQFADVLFRPGGPL
                     DPAKYYIILPDGIGHGGSSKPSDGLRAHFPAYDYDDMVEAQRRLLVDGLKVDHLRLIM
                     GTSMGCMHAFVWGEAHPDFADALMPLACLPTEIAGRNRVWRKLAMDGIRADPAWLGGD
                     YAEQPLQGLRTAQSLLMLAGGAPMPMQRELAGRAAADAWVEQGMAAALPRLDANDLWF
                     QINASRTYDPSAGLERITAPVMWVNSADDFINPPELGLAETFAPRIKRGTYVLLPNSA
                     DGHGHGTHTWAAFWQDRLAALLKASEPRP"
     gene            complement(3381..>3496)
                     /locus_tag="PLO65_12980"
     CDS             complement(3381..>3496)
                     /locus_tag="PLO65_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRD29206.1"
                     /translation="ARLDMTALVDGYYAEVQAMGGSRWDTSSLAARLEKPQ"
BASE COUNT          526 a         1261 c         1233 g          476 t
ORIGIN      
        1 attcgctgac cgacgccgga ctgccggtcc aggcgctgca ggtgatccgg tcggggttgc
       61 gggacgccgg gcggtcggac gacgccgcgg ccctcaagct cggcctggcc gacgccggcg
      121 cctacgcctc gcgcgaactg ggcgacctgc ccgccttcat cgacaacatc gacatcggca
      181 tcgccgccac cctcgcgacg gaccagccga tcgacggccg caccgcgctc tacaacctga
      241 ccgtcctgtc ttcgagccag ggccgcaacg cgctggcgga gcggttgctg cagcagtacc
      301 gcaggctcgc cgacgagacc ggcaacggca tcgagatcgg ctgggccaat gagctctgcg
      361 ccaagatcaa gtcggccaac cggcgctacg ccgcggccct gcaatgcgcc gcggcggccc
      421 tgaccaaccc cgatatcgcc gccgagcacc ggcccaaggt catgctgctc gaggtgatcg
      481 ccctggcccg gctcggccgg ccggccgagg cgcggcggca gcttcaggcg ctgaccgacc
      541 tcgcccgcga gcggggtgac ccggtgctga tgcatgacct gctgcagggc gaggccgagg
      601 tgctgcggag cgagggccgc ctggccgagg cctacgaggc cctgatgcgg ttccacgacc
      661 agcagcgcag cgacatggcc gcctcggtcg tggaggggct gcacggcatg cgcgccagcc
      721 tcgagagcga gatcgacgac acccaggccc tgctggacgc ccagcgccgc cagaccctgc
      781 agatcagcgt cctggtcggg gtggtggccc tggcgctgat cgcggccgtg gtctcgctgc
      841 tcaatcaacg acgactgcag ggccggctgg tggcggcggc cgagcgggcc gagcgggccg
      901 accgggtcaa gtccgagttc ctcgccaaca tgagccacga gatccgcacg ccgctgacca
      961 gcatcgtcgg gtattcgcgc ctgctggccg accagcccga cctgtcgccc gattcagcgc
     1021 agttcgccgg ccgaatcgtc accgccaccc agtcgttgct ggcgatcgtc aacgacgtgc
     1081 tggacttctc gaagatcgag gccggtcagg cgcggatcga accccgcccc accgcgctcg
     1141 cgggcctggt gcaggatgtc gccggactgt tcggggccca ggcggcgaac aagggcgtca
     1201 ccctcaccgt cacgctggcc gaagacctgc cggcctggac actggtcgat cccgaccggg
     1261 tgcgccaggt cctgctgaac ctggtgggca acgcggtgaa gttcaccgcc gccggcgagg
     1321 tgcgggtcag cgcggcctgg tcgtcggacg acggcctgcg gatcgatgtc gtcgacagcg
     1381 gccccggcat ctcggcggaa ggacaggccc gcctgttcca gcgcttttcc caggttcacg
     1441 gcgccagcaa ccgcatggag agcggcacgg ggctcggcct ggccatctgc tacggcctgg
     1501 tcaccgccat gggcggcgag atcggcgtgg aaagccagcc cggcgagggc agccgcttct
     1561 ggttccgcac gcccgccccg ctctccgacg cccccgccac ggccgaaatg ccagaactcg
     1621 tcgcggactc cccgtcggac cgggcgcggg tgctcgtcgt cgacgacaac gaggccaacc
     1681 gggagctggt gtcccatctg ctgcggccgc tggacctcga cctgagcttc gccacgggcg
     1741 gcgaggaggc cgtgcagacg gcgatggtcg atgtgttcga cctgatcctg atggacatcc
     1801 gcatgccggg catgggcggc gaggaggcca tgggccggat ccgcagcggc gccggcccca
     1861 acgccgaggc cccgatcctc gccttcaccg ccgatgtcga cggccagaac accgaacgcc
     1921 tgctcggcgc cggcttcgat ggccatgtgc acaagccgat cgacgcccgc ggcctgatca
     1981 cctcggtcgt ccagtggacc gcccgcgcgg cctaggcgga cgggccctca tagtcgaact
     2041 cgaccaggaa cacccgctcg cccggcccat gcctggcgac cttgagcagg tccacaaggc
     2101 ctgaaaaccc cgagcgccgg gccaggtcgg gcgtcacatc ggccagcgat atctcgtgaa
     2161 tcgagcggac cacgacatgc cccggcccga gcgcgtatcg cccgcccgcc ttgacgcgcg
     2221 ggctctgcca gatgcgcacc gagcaggtca cctccccgcg catcacgggc tcccgcaggc
     2281 gggccgcgaa catcacgggc ggggttccga cgccttcagc agcgcggcca gccggtcctg
     2341 ccagaaggcc gcccaggtgt gggtcccatg gccgtggccg tcggcgctgt tgggcagcag
     2401 gacgtaggtt ccgcgcttga tccggggcgc gaaggtctcg gcgagaccca gttccggcgg
     2461 attgatgaag tcgtccgccg aattgaccca catcaccggc gccgtgatcc gctccagccc
     2521 ggccgagggg tcgtaggtcc gcgaggcgtt gatctggaac cacaggtcat tggcgtccag
     2581 ccgcggcaag gcggcggcca tgccctgttc cacccaggcg tccgcggcgg cgcggccggc
     2641 cagttcgcgc tgcatcggca tgggcgctcc gccggccagc atcagcaggc tctgggccgt
     2701 gcgcaggccc tgcagcggct gctcggcgta gtcgccgccc agccaggccg ggtcggcgcg
     2761 gatgccgtcc atggccagtt tgcgccagac ccggttgcgg ccggcgatct ccgtcggcag
     2821 gcaggccagc ggcatcaggg cgtcggcgaa gtcgggatgg gcctcgcccc agacgaaggc
     2881 gtgcatgcag cccatcgagg tgcccatgat cagccgcaga tggtcgacct tgagcccgtc
     2941 gaccagcaac cggcgctggg cctcgaccat gtcgtcatag tcataggccg gaaaatgcgc
     3001 gcgcaggccg tcgctgggct tggaggaccc gccgtggccg atgccgtcgg gcaggatgat
     3061 gtagtatttc gccggatcaa gcgggccgcc tggccggaac agcacatcgg cgaactgcgg
     3121 ccgcaggaac tgcgcgcccg agccacccgt gccgtgcagg atcatcaccg cgttgaccac
     3181 ccggccggag gcgtcgcgcc gcggttcgcc cagggtgcgg tagtgcagcc tcaggtccgg
     3241 cagggtctcg ccggactgga agcggaagtt ccggacgacg acgtcgcctt ccgtcacgac
     3301 cggcgcggcc agggccgggg cggccaggac catcgacagg ccgatcgcca gggtggcgag
     3361 gcgggtgaag gtcgaccgga tcattggggt ttctccagtc gcgcggccag gctggaggtg
     3421 tcccagcggc ttccgcccat cgcctggacc tcggcgtagt agccgtcgac cagggcggtc
     3481 atgtccagtc gcgcgc
//
LOCUS       DAORNX010000332         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20337_length_3487_cov_8.368007, whole genome shotgun sequence.
ACCESSION   DAORNX010000332 DAORNX010000000
VERSION     DAORNX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20337_length_3487_cov_8.368007"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLO65_12985"
     CDS             complement(<1..228)
                     /locus_tag="PLO65_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29207.1"
                     /translation="MTANAIEGLRLFLVEDEALVAMLLEDMLADLGCVVVDTAGSLDQ
                     ALGRLEDIAGRADGAILDVNLGGEQVYPFADA"
     gene            complement(225..1736)
                     /locus_tag="PLO65_12990"
     CDS             complement(225..1736)
                     /locus_tag="PLO65_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD29208.1"
                     /translation="MFEAAPAPYLLLAGDPPRYTIVDVNTAYMAATGTRREAIVGRPL
                     FEVFPDNPEDLEADGVGGLRASLDRVVRDRTADVMGLQKYDIPLPDHPETFEVRYWSP
                     INTPVTGPGGEVTHIIHRVEDVTEFVLAQQGVDRIEALADRRQAEVLRNAADLREANR
                     QLKEVTGRLSELNALQAAQAQARLSFAMNSAGMGELILDPATDRTVHSPGMAKLLGFA
                     GDRLLTREEIRQAVHPEDQAQVAAHRDAAIAGPGEAFETEHRVVWPDGSVRWLVNRGR
                     VARDGDGRATEITAVFMDITERKQAEERQTLLLAELNHRMKNTLATVMALAGQTRRGA
                     ADMESFGRTFEARLRALSTAHDLLSEGSWNGASLADVLLRTLAPYSATPGRVRAEGPA
                     IRLSPNAAVTMSMAIHELATNAAKYGALSTGDGRLSVDWTVDISPAPGRLAIRWVETG
                     GPAVATPPRRGFGSRLIEQALARELGGEARLLFPPEGVQCEIDLPLSHKVSPG"
     gene            complement(1917..2492)
                     /gene="purN"
                     /locus_tag="PLO65_12995"
     CDS             complement(1917..2492)
                     /gene="purN"
                     /locus_tag="PLO65_12995"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRD29209.1"
                     /translation="MSKARVAILISGAGSNMAALVEASRAAGCPYEVVLVVSNNPDAA
                     GLSKAATLGVPTLAIDHRPFGKDREAHERRIDAALRAAGADYVALAGYMRVLTPFLVE
                     AWAGRMVNIHPSLLPKHPGLNTHQRAIDGGDPEAGCSVHLVTAGVDEGPVLGQAAVPV
                     RPDDTAETLAARVKAEEHQLYPACLAALTRG"
     gene            complement(2489..>3487)
                     /gene="purM"
                     /locus_tag="PLO65_13000"
     CDS             complement(2489..>3487)
                     /gene="purM"
                     /locus_tag="PLO65_13000"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRD29210.1"
                     /translation="TYAQAGVDIDAGNALVEAIKPLARATARPGMQGGLGGFGALFDL
                     KAAGYEDPLLVSTTDGVGTKLKIAIETGLHDTVGIDLVAMCVNDLLAQGAEPLLFLDY
                     FATGRLDVATATRVVAGIAEGCRLAGCALVGGETAEMPGMYADGEYDLAGFTVGAVNR
                     DGVLPRLDHQRAGDVLIGLASSGPHSNGYSMVRKVVERSGLAWDGPSPFGPGTLAESL
                     MIPTRIYVKSTLPLIKAGKIKGVAHITGGGLIENPPRAIAEGLAPRFDWNAWSPGPTF
                     QWLQETGGIAEHEMRRTFNCGIGFLLIVARDEVPAVLADLLDAGEDAFVCGELAKG"
BASE COUNT          500 a         1238 c         1221 g          528 t
ORIGIN      
        1 ggcgtcggcg aacgggtaga cctgctcgcc gccgaggttg acgtcgagga tcgcgccatc
       61 ggcccgcccg gcgatgtcct ccagccggcc cagcgcctgg tccaggctgc cggcggtgtc
      121 gaccaccacg catcccagat cggcgagcat gtcctcgagc agcatggcca cgagggcttc
      181 gtcttcgacc agaaacagcc gaaggccttc gatcgcgttg gccgtcatcc gggcgacacc
      241 ttgtgtgaca ggggcaggtc gatttcacat tgcacgccct cgggcggaaa aagcaggcgg
      301 gcctcgccgc caagctcgcg cgcgagcgcc tgttcgatca gccgcgagcc gaagccgcgc
      361 cggggcggcg ttgcgacggc cggcccgccg gtctcgaccc agcggatggc caggcggccc
      421 ggggcggggg atatgtccac ggtccagtcc accgacagcc gtccgtcccc cgtcgacagg
      481 gcgccgtact tcgcggcgtt ggtcgccagt tcatggatcg ccatgctcat ggtcacggcg
      541 gcgttgggac tcaggcggat ggcgggccct tcggcccgca cccgccccgg cgtcgcgctg
      601 tagggggcca gcgtcctgag cagcacgtca gccagcgagg ccccgttcca ggacccctcg
      661 ctgagcaggt catgggcggt ggacagggcc cgcagccgcg cctcgaaggt cctgccgaag
      721 gactccatgt cggccgcgcc gcgccgcgtc tggccggcga gcgccatcac cgtggcgagg
      781 gtgttcttca tccggtgatt cagttccgcc agcagcaggg tctggcgttc ctcggcctgc
      841 ttgcgttcgg tgatgtccat gaacaccgcg gtgatctcgg tcgcgcgtcc gtcgccgtcg
      901 cgcgctaccc gaccccggtt gaccagccag cgcaccgacc cgtcgggcca gaccacgcgg
      961 tgctcggtct cgaaggcctc gcccggacca gcgatggccg cgtcgcgatg ggcggcgacc
     1021 tgggcctggt cttccggatg cacggcctgg cggatttcct cgcgcgtcag cagccggtcc
     1081 ccggcgaacc cgagcagctt ggccatgcca ggcgagtgca ccgtgcggtc cgtggccgga
     1141 tccaggatca gttcgcccat acctgccgag ttcatggcga agctgagccg ggcctgggcc
     1201 tgcgccgcct ggagggcgtt cagctccgac agccgaccgg tcacctcctt gagctggcgg
     1261 ttggcctcgc gcagatcggc tgcgttgcgc agaacctcgg cctgccgtcg gtcggcgagc
     1321 gcctcgatac ggtcgacgcc ctgctgcgcc agcacgaact cggtgacgtc ctcgacccgg
     1381 tggatgatgt gcgtcacctc accgcccgga ccggtcacgg gcgtgttgat cgggctccag
     1441 tagcggacct cgaaggtctc gggatggtcg ggcagcggga tgtcgtactt ctgcaacccc
     1501 atgacatcgg cggtccggtc gcgcacgact cggtccagcg aggcgcgcag gccgccaacc
     1561 ccgtccgcct caaggtcctc gggattgtcc ggaaacacct cgaacagagg gcgaccgaca
     1621 atcgcctctc ggcgggttcc ggtcgccgcc atgtaggcgg tgttgacgtc gacgatggtg
     1681 tagcgcggcg gatcgcccgc cagcagcagg tagggggcgg gcgccgcttc gaacacccgc
     1741 cgatagtcaa cggccgcggg cgccctggac gccccgtctc ggctggccgg ttgtctgggt
     1801 tcgctggtca ccgatgccga tcatttcgcc gcgatcgccc tgttcgacgc caggcagggc
     1861 ggacgccaac gagccccacc cgtatacgtt gattctgtcg gggcagttcg cgcgcgtcag
     1921 ccgcgcgtca gcgccgccag gcaggccgga tagagctggt gttcctcggc cttcacgcgg
     1981 gcggccaggg tttcggcggt gtcgtccggc ctgaccggaa ccgcggcctg acccagcacg
     2041 ggaccctcgt ccacgccggc ggtgaccaga tgcaccgagc aaccggcctc ggggtcgccg
     2101 ccgtcgatcg cccgctggtg cgtgttcagg ccggggtgct tcggcaacag cgagggatgg
     2161 atattgacca tccgaccggc ccaggcctcg acgaggaagg gcgtcagcac ccgcatgtat
     2221 ccggccaggg cgacatagtc cgccccggcc gcgcgcaacg ccgcgtcgat ccgccgttca
     2281 tgggcctcgc gatccttgcc gaagggccga tggtcgatcg ccagggtcgg cacgccaagc
     2341 gtcgcggcct tcgaaagacc cgcggcgtcg gggttgttgg agacaaccag caccacctcg
     2401 tagggacagc cggccgcgcg cgaggcctcc accagggcgg ccatgttcga ccccgcgccg
     2461 gagatcagga tggcgacgcg agccttgctc accccttggc cagttcgccg cagacgaagg
     2521 cgtcctcgcc cgcgtcgaga aggtcggcca gcaccgcggg gacctcgtca cgggcgacga
     2581 tcagcaggaa gccgattccg cagttgaagg tgcggcgcat ctcgtgctcg gcgatgccgc
     2641 cggtctcctg cagccactgg aaggtcgggc cgggactcca ggcgttccag tcgaagcgtg
     2701 gagccagccc ctcggcgatg gcgcgcggcg gattctcgat cagccccccg ccggtgatat
     2761 gcgccacacc cttgatcttg ccggccttga tcagcggcag ggtcgatttc acgtagatcc
     2821 gcgtcgggat catcaggctc tcggccagcg tgccgggccc gaacggggag ggcccgtccc
     2881 aggccaggcc cgagcgctcg acgaccttgc gaaccatcga atagccgttg gagtgcggtc
     2941 cgctcgaggc caggccgatc agcacgtcgc cagcccgctg gtggtccagc ctgggcagca
     3001 cgccgtcgcg attgacggcc ccgacggtga agccggcgag gtcgtactca ccgtcagcgt
     3061 acatgcccgg catctcggcc gtctcgccgc cgacgagggc gcaaccggcc agcctgcagc
     3121 cctcggcgat gccggcgacc acccgggtgg ccgtggccac gtccagccgg ccggtggcga
     3181 aatagtcgag gaacagcagc ggctcggcgc cctgggccag caggtcgttg acgcacatgg
     3241 ccaccagatc gatgccgacc gtgtcgtgca ggccggtctc gatggcgatc ttcagcttgg
     3301 tgcccacccc gtcggtggtc gagaccagca gcgggtcctc gtagccggcg gccttgaggt
     3361 cgaacagggc gccaaagccg ccgagcccgc cctgcatgcc gggtcgggcg gtggccctgg
     3421 ccagcggctt gatcgcctcg acgagcgcgt ttccggcgtc gatgtcgaca ccggcctggg
     3481 cgtaggt
//
LOCUS       DAORNX010000333         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20377_length_3481_cov_7.397256, whole genome shotgun sequence.
ACCESSION   DAORNX010000333 DAORNX010000000
VERSION     DAORNX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20377_length_3481_cov_7.397256"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1759)
                     /locus_tag="PLO65_13005"
     CDS             complement(74..1759)
                     /locus_tag="PLO65_13005"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRD29211.1"
                     /translation="MFEGLSHSARDAKAWPFEQARNLLARVLRVRLDDAERDLASTLI
                     NSGKADEAFRTFEALQRPVVLETGYGPSGLPHMGTFGEVARTTMVRMAFRALTDDALP
                     TRLISFSDDMDGLRKVPPNVPNGELLALDLDKPLTVVRDPFGEHESFGHHNNARLRAF
                     LDSFGFDYEFVSSTDCYRGGRFDQTLLTALARFDAIQKIMLPSLGEERRATYSPFLPI
                     SPRTGKVLQVPTLERHVDRGTIVFRDENGELTEVPVTGGGVKMQWRPDWAMRWTALGV
                     DYEMSGKDLVDSVRLSNQVCKVLGGTPPEGFNYELFMDEHNHKISKTKGNGLTMEEWL
                     RYGAPESLAYYMFQSPKSAKKLYFDVIPKATDEYLQQLDAFNRKRSEGDNAPDLNNPA
                     WHVHMGQPPQTGSPVSFSLLLNLVSAGNASDREVLWGFLSRYIPGATPRSEPLLDRLV
                     DYAMNYYEDFVAPTKAFRAATDQERAAMQDLLARLRALPTGCQDAEQIQFEVYEAGKA
                     HGFDPLRLWFQGLYEVLLGQTQGPRFGSFAAIFGLDRTVQLIEDGLAGKLLAA"
     gene            complement(1792..2325)
                     /locus_tag="PLO65_13010"
     CDS             complement(1792..2325)
                     /locus_tag="PLO65_13010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HRD29212.1"
                     /translation="MIGTLSDLFRRLMAALTTWPSPGQWLAAAGVGLVALVLEGAIGL
                     GGGLLRPAPSDWSVLPFSLALALVVPALGEETVFRGVLIPSRGDSRDATLAILLSTLA
                     FVGWHVLEALTFMRAAAPVFLRGDFLATTAVLGLACGVLRHRTGSLWPAVALHWLEVA
                     IWQIGFGGGQVARALVP"
     gene            2511..2900
                     /locus_tag="PLO65_13015"
     CDS             2511..2900
                     /locus_tag="PLO65_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD29213.1"
                     /translation="MELPITSALAGAAAIFLVLLSFPVANHRRARGVSFGDGGDEKLT
                     RLIRAQANFVEYAPLAIVAIGLVEMGGASPLLVCGLAAALAAGRLLHAFGLLANMLVG
                     RALGTVLTFAVLLVAGGLLLAGQLGLG"
     gene            complement(2897..>3481)
                     /locus_tag="PLO65_13020"
     CDS             complement(2897..>3481)
                     /locus_tag="PLO65_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRD29214.1"
                     /translation="AILSLITAGISLLPYLLVAVARGSALGAFARAVAPVQALAFSTQ
                     SSLACLPAMLERAQDDLKIPPRVTGLVLPLAVAVFRLTGPAANLSVAYFCAHIYGVHP
                     GLPQMIGAVFIAWAISVGSVGLPGQVSFMASIVPICIALGVPVDLLPILLAVEVAPDI
                     FRTIGNVTGDLAAATVLHRPGDEVEAADPESVPT"
BASE COUNT          541 a         1186 c         1250 g          504 t
ORIGIN      
        1 cgtcgcccgt ctgtgaggag gaccatgcca ccccccgcct gaacgccgac ggggcggggc
       61 tgtcaggttt cgttcaggcc gccagcagct tgccggcgag gccgtcctcg atcagctgga
      121 cggtccggtc gaggccgaag atggccgcga acgagccgaa gcgcgggccc tgggtctggc
      181 ccagcagcac ctcgtagagc ccctggaacc acaggcgcag cgggtcgaag ccatgcgcct
      241 tgccggcctc gtagacctcg aactggatct gctcggcgtc ctggcatccg gtcggcaggg
      301 cgcggaggcg ggccagcagg tcctgcatcg ccgcccgctc ctggtcggtg gcggcccgga
      361 aggccttcgt cggggcgacg aagtcctcgt agtagttcat cgcatagtcg accaggcggt
      421 cgagcagcgg ctcgctccgc ggcgtcgcgc cggggatgta gcggctgaga aagccccaca
      481 gcacctcgcg gtccgaggcg ttgccggccg agaccaggtt cagcaacagc gagaacgaca
      541 ccggcgagcc ggtctgtggc ggctgaccca tgtggacgtg ccaggccggg ttgttcaggt
      601 ccggcgcgtt gtccccctca gaccgcttgc ggttgaaggc gtcgagctgc tgcaggtact
      661 cgtccgtcgc cttggggatg acgtcgaaat agagcttctt ggccgatttc ggcgactgga
      721 acatgtagta ggcgaggctc tcgggcgcgc cgtagcgcag ccactcctcc atggtcaggc
      781 cgttgccctt ggtcttggag atcttgtggt tgtgctcgtc catgaagagc tcgtagttga
      841 acccctcggg cggcgtgccg ccgagcacct tgcagacctg gttggagagc cggacgctgt
      901 cgaccaggtc cttgccgctc atctcgtagt cgacgccgag cgcggtccag cgcatggccc
      961 agtcgggccg ccactgcatc ttcaccccgc cgccggtgac cgggacctcg gtcagttcgc
     1021 cgttctcgtc gcggaacacg atcgtgccgc ggtcgacgtg gcgctccagc gtcgggacct
     1081 gcagcacctt gccggtcctg gggctgatcg gcaggaacgg cgagtaggtc gcgcgccgct
     1141 cctcgccgag cgagggcagc atgatcttct ggatcgcgtc gaaccgggcc agcgccgtca
     1201 gcagcgtctg gtcgaaccgg ccgccgcgat agcagtcggt cgaggagacg aactcgtagt
     1261 cgaagccgaa gctgtcgagg aaggcgcgca ggcgggcgtt gttgtggtgg ccgaaactct
     1321 cgtgctcacc gaacgggtcg cgaaccacgg tcaggggctt gtcgaggtcg agcgccagca
     1381 gctcgccgtt gggcacattg ggcgggacct tgcgcaggcc gtccatgtcg tcgctgaagc
     1441 tgatcagccg ggtcggcagg gcgtcgtcgg tcagggcgcg gaaggccatc cgcaccatgg
     1501 tcgtgcgggc cacctcgccg aaggtgccca tgtgcggcag gcccgaggga ccatagccgg
     1561 tctccagcac cactggtcgc tgcagggcct cgaaggtcct gaacgcctcg tcggccttgc
     1621 ccgaattgat cagggtcgac gccaggtcgc gctcggcgtc gtcgaggcgc acgcgcagca
     1681 cgcgggccag caggttgcgc gcctgctcga acggccaggc cttggcgtcg cgggcggagt
     1741 gggaaaggcc ttcaaacatg gggcgggttt gtagggacaa tggccgccgg gtcaaggaac
     1801 cagcgccctg gcgacctggc cgccgccaaa gccgatctgc cagatcgcca cctccagcca
     1861 gtgcagggcc acggccggcc acagcgagcc ggtccggtga cgcaggacgc cgcaggcgag
     1921 acccagcacc gcggtcgtgg ccaggaagtc gccgcgcaga aacaccggcg ccgcggcccg
     1981 catgaaggtc aacgcctcca ggacgtgcca gccgacgaag gccagggtcg acaacagaat
     2041 ggccagggtc gcgtcccgac tgtccccccg ggacgggatc agcacgccgc gaaagaccgt
     2101 ttcctcgccg agggccggaa cgaccagggc cagcgccaga ctgaacggca ggaccgacca
     2161 gtccgacggg gccggcctga gcaggccgcc ccccaggccg atcgcgccct cgaggaccag
     2221 cgcgaccagg ccgacgcccg cagccgccag ccactgcccg ggcgaaggcc aggtcgtcag
     2281 ggcggccatc agccggcgga agaggtcgga cagtgtgccg atcatgcagc catcataggc
     2341 ccgcgccgcc gccgtggggg accaccaccg ggcgatttcg ggcgaaagtc ccgccaatgc
     2401 cccgcgatgt cccgacgctt ttcgtcccga gagtcccgct catcgcgcgt gcattttctc
     2461 atccaccggt tcagactcgc gttcgtcgaa cctggggagg gctatacgac atggaactgc
     2521 cgatcacgtc tgcgctggcg ggcgccgccg ccatattcct ggtgctgctg agctttccgg
     2581 tggccaacca tcgccgcgcc aggggcgtga gttttggcga cgggggcgat gagaagctca
     2641 cccgcctgat ccgggcccag gccaatttcg tcgagtacgc gccgctggcc attgtcgcca
     2701 tcggtctggt cgagatgggc ggcgcctcgc cgctgcttgt ctgcggcctg gccgcggccc
     2761 tggccgccgg acgactgctg catgccttcg gcctgctcgc caacatgctg gtcggccggg
     2821 cgctcggcac ggtcctgacc ttcgccgtcc tgctggtcgc cggcggcctg ctgctggccg
     2881 gacagctcgg gctgggctag gtcggtacgg actctgggtc ggccgcctcg acctcatcgc
     2941 ccggccggtg cagcacggtc gcggcggcga ggtcgccggt gacgttgccg atggtgcgga
     3001 agatgtcggg cgccacctcg accgccagca ggatcggcag caggtcgacc ggcacgccca
     3061 gggcgatgca gatcggcacg atgctggcca tgaacgacac ctggcccggc aggccgaccg
     3121 agccgacgct gatcgcccag gcgatgaaca ccgccccgat catctgcggc aggcccgggt
     3181 ggacgccata gatgtgggcg cagaaatagg ccaccgacag gttggccgcc gggccggtca
     3241 ggcggaacac ggccacggcc aggggcagca ccaggcccgt gacgcgcggc gggatcttca
     3301 ggtcgtcctg ggcccgctcg agcatggccg gcaggcaggc cagcgacgac tgggtgctga
     3361 aggccagcgc ctgcaccggg gccacggccc gggcgaaggc gcccagggcc gagccgcggg
     3421 ccacggccac cagcagatag ggcagcaggc tgatgccggc ggtgatcagc gagaggatgg
     3481 c
//
LOCUS       DAORNX010000334         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20384_length_3480_cov_6.735182, whole genome shotgun sequence.
ACCESSION   DAORNX010000334 DAORNX010000000
VERSION     DAORNX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20384_length_3480_cov_6.735182"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLO65_13025"
     CDS             <1..936
                     /locus_tag="PLO65_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD29215.1"
                     /translation="LPSVRQGAARGLVAAAAQMLGDRQYEMAIAYFRMGLYLDPDRDE
                     GRVLLGDALAASKEPEAARAAYAAVGPHSPQYTVARAKLAWTYQQAKEGETALRLARE
                     GFDSAPTNDNAALTYADLLRANDRNAEAVTVLDAVIGRAGPAPDWRLLYMRGVALERI
                     GRWPEAEKDLRAALASQPNEPELLNYLGYSWIDRGERLAEALDMVKRAVAANPRSGPI
                     VDSLGWAYYRLGDYRNAVEQLETAVLLEPADPEINNHLGDAYWRVGRRIEAEFQWKRV
                     LSLDPPPKIKAEAEAKLKSGLGPVGPAPAPVTAAN"
     gene            1084..1962
                     /locus_tag="PLO65_13030"
     CDS             1084..1962
                     /locus_tag="PLO65_13030"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRD29216.1"
                     /translation="MALSAFAPAKVNLFLHVGPVAADGYHPLSSLMVFADIGDTLSIQ
                     PSETPGFEVTGPYGGQVESGPGNLVVQAARALFEHVKGPQPPFRLILDKQLPVAAGLG
                     GGSSDAGATLRLLRSALDLAVQDEMLTAIAARLGADGPVCLWGRAAMTSGRGDRLAPP
                     PGLPPLNAVLVNPGVPSSTAGVYRAFDEAGFMGDATAPSLPSAFESAEETAAFMAWCR
                     NDLEAPAVSLAPAIGEVLETLRGEPEVLLARMSGSGATCFALCADDIQAEGLAERLEQ
                     MRPDWWVRRCRLGNGI"
     gene            complement(2059..>3480)
                     /locus_tag="PLO65_13035"
     CDS             complement(2059..>3480)
                     /locus_tag="PLO65_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRD29217.1"
                     /translation="ATAAPAAEPAATPAPAAAAPAAEPTITDTVDKGDNAWMMVSSLL
                     VLLMIIPGLALFYGGLVRQKNMLSMLMQVSTVAVVGMITWALWGYSLAFTDGGAADKF
                     VGGLGRLFLGGVTPASNVATFSTGVVIPEFTFISFQMTFACITAALVLGGLAERIKFS
                     ATVLFAVIWPILVYYPMAHMVWWWAGPDAVALHPKEPIQSGLLWGMGALDFAGGTVVH
                     INAGIAALVGAIFLGKRSGYQKEPMPPHSLTLTLVGAGLLWVGWFGFNAGSNLEANGY
                     ASLAMINTFLATASAGFSWVLVEWVTRKKASMLGLASGMVAGLVAVTPASGFAGPMGA
                     LILGLIVSPICVFFCSVIKNALKYDDSLDAFGIHAVGGIVGALATGLLVNPDWGGAGI
                     VDYTTCGLDGDLSTCDASTTYTMASQFLIQLKAVLITIAWSGIGSSIVFFVIKLITGL
                     RVTPETEEEGLDISEHGERAYHS"
BASE COUNT          554 a         1184 c         1250 g          492 t
ORIGIN      
        1 ctgccgtcgg tccgccaggg cgcggcccgg ggtctggtcg cggccgccgc ccagatgctc
       61 ggcgaccggc agtacgagat ggccatcgcc tatttccgca tgggcctcta cctcgacccg
      121 gaccgcgacg agggtcgcgt cctgctgggc gacgccctgg ccgccagcaa ggagcccgag
      181 gcggcccgcg cggcctatgc cgcggtcggt ccccactcgc cgcaatacac ggtcgcccgc
      241 gccaagctgg cctggaccta ccagcaggcg aaggagggcg agactgccct gcggctggcc
      301 cgcgagggct tcgactcggc cccgaccaat gacaatgcgg ccctgaccta cgccgacctg
      361 ctgcgcgcca acgaccgcaa tgcagaggcg gtgaccgtgc tcgacgcggt catcggccgc
      421 gccggtccgg ctcccgactg gcgattgctc tacatgcgcg gcgtggcgct ggagcggatc
      481 ggccgctggc ccgaagcgga aaaagacctg cgcgccgccc tcgccagcca gcccaacgaa
      541 ccggagctgc tcaactacct cggctacagc tggatcgacc ggggcgagcg gctggccgag
      601 gccctcgaca tggtcaagcg cgcggtcgcc gccaacccgc gctcggggcc catcgtcgac
      661 tccctgggct gggcctacta ccgcctcggc gactaccgca acgccgtgga acagctggag
      721 acggcggttc tgctcgaacc ggcggatccg gagatcaaca accacctggg cgacgcctac
      781 tggcgggtcg gccgccggat cgaggcggaa ttccagtgga agcgggtgct cagcctcgat
      841 ccgccgccga agatcaaggc cgaggccgag gccaagctca agtctggcct gggaccggtc
      901 ggacccgccc ctgcgccggt gacggcggcg aactgatccg aataggttac gtccttcgac
      961 acggccgctg gcgcggcctg ctcaggatga cgtaatcagg catacgtcat ggtgggcagc
     1021 gccgcaggcg cgtgtcgaac cacgcacgct ctcgacgccc tcgggctagc tgtggaatcc
     1081 ggaatggccc tgtcagcctt cgcgccggcc aaggtgaacc tgttcctgca tgtcgggccg
     1141 gtggcggcgg acggctatca tccgctgtcg agcctgatgg tcttcgccga catcggcgac
     1201 accctgtcga tccagccgtc cgagacgccc ggcttcgagg tcaccgggcc ctacggcggt
     1261 caggtcgaga gcggtcccgg caacctggtg gtccaggccg cccgcgcgct gttcgagcat
     1321 gtcaagggac cgcagccgcc gttccggctg atcctcgaca agcagctgcc ggtggccgcg
     1381 ggcctgggcg ggggatcgtc ggacgccggg gcgaccctgc ggctgctgcg ctcggcgctg
     1441 gacctggcgg tgcaggacga gatgctgacg gcgatcgccg cgcgcctggg cgcggacggg
     1501 ccggtctgcc tgtgggggag ggccgccatg acctccggac ggggcgaccg gctggcgccg
     1561 ccgccggggt tgccaccgct gaacgccgtg ctggtcaatc ccggggtccc gtcttcgacg
     1621 gccggcgtct atcgcgcgtt cgacgaggct ggcttcatgg gcgacgcgac ggcgccgtcg
     1681 ctgccatcgg cgttcgagag cgccgaggaa acggcggcct tcatggcctg gtgccgcaat
     1741 gatctggagg cgccggccgt cagtctggcg ccggcgatcg gcgaggtgct ggagaccctg
     1801 cgtggcgagc cggaggtgct gctggcccgc atgagcggct cgggcgccac ctgcttcgcc
     1861 ctctgcgccg acgacattca ggccgagggg ctggcggaac ggctggagca gatgaggccg
     1921 gactggtggg tccggcggtg ccggctggga aacgggatct agggacacga aaaaggggac
     1981 atgcaccgca ggtacgtgtc ccccattcga gccgccggga taggggacac gtacctgcgg
     2041 tacatgtccc cctgtttcct agctgtggta ggcgcgttcc ccgtgttcgg agatgtccag
     2101 gccttcctct tcggtctccg gcgtgacgcg caggccggta atcagcttga tcacgaagaa
     2161 cacgatgctg ctgccgatgc ccgaccaggc gatggtgatc agcacggcct tcagctggat
     2221 caggaactgc gacgccatgg tgtaggtggt cgaggcatcg caggtcgaca ggtcgccgtc
     2281 gaggccgcag gtcgtgtagt cgacgatgcc cgcgccgccc cagtccgggt tgaccagcag
     2341 gccggtggcc agggcgccga cgatgccgcc gaccgcgtgg atgccaaacg cgtccaggct
     2401 gtcgtcgtac ttcagcgcgt tcttgatcac cgagcagaag aacacgcaga tcgggctgac
     2461 gatcaggccc aggatcaggg cgcccatcgg gccggcgaag ccggaggccg gggtgacggc
     2521 cacaagaccg gcgaccatgc ccgaggccag gccgagcatc gaggccttct tgcgcgtgac
     2581 ccactcgacc agcacccagg agaagcccgc cgaggcggtc gccaggaagg tgttgatcat
     2641 ggccagggag gcgtagccgt tggcttcgag gttggagccg gcgttgaagc cgaaccagcc
     2701 cacccacagc agaccggcgc cgacgagggt cagggtcagg ctgtgcggcg gcatcggctc
     2761 cttctggtag ccgctgcgct tgccgaggaa gatggccccg acgagggccg cgatcccggc
     2821 gttgatgtgc accacggtgc cgccggcgaa atccagcgcg cccatgcccc acagcagacc
     2881 ggactggatc ggctccttcg gatgcagggc gacggcgtcg gggcccgccc accaccagac
     2941 catgtgggcc atcggatagt agacgaggat cggccagatg accgcgaaca gcacggtggc
     3001 gctgaacttg atccgctcgg ccaggccccc cagcaccagc gccgcggtga tgcaggcgaa
     3061 ggtcatctgg aagctgatga aggtgaactc ggggatcacg acaccggtcg agaaggtcgc
     3121 cacgttggag gccggcgtca cgccgccgag gaacagccgc cccaggccgc cgacgaactt
     3181 gtcggccgcg ccgccgtcgg tgaaggccag gctgtagccc cacagcgccc aggtgatcat
     3241 gccgaccacc gcgacggtgg agacctgcat gagcatggac agcatgttct tctgccgcac
     3301 caggccgccg taaaacagcg ccaggcccgg gatgatcatc agcaggacga gcaggctgga
     3361 gaccatcatc caggcgttgt cgcccttgtc gaccgtgtcg gtgatcgtcg gttcggcggc
     3421 cggcgccgca gcggccggcg ccggcgtggc ggcgggttca gcggccggag ccgcggtggc
//
LOCUS       DAORNX010000335         3466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20498_length_3466_cov_7.605394, whole genome shotgun sequence.
ACCESSION   DAORNX010000335 DAORNX010000000
VERSION     DAORNX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20498_length_3466_cov_7.605394"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLO65_13040"
     CDS             <1..304
                     /locus_tag="PLO65_13040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD29218.1"
                     /translation="VLEAMACGLPVVGVDAGGVAESVDHAVGQLAWRSDSAALAEAVA
                     ALFERDLALVGRTARTRAETRHGWNRVFEQLAAIYARLTCDTAFVTPPPALTRSSR"
     gene            complement(267..749)
                     /locus_tag="PLO65_13045"
     CDS             complement(267..749)
                     /locus_tag="PLO65_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD29219.1"
                     /translation="MRRGRSLIGRVRERLHALTWASHRQQYAALPWRRGKDGIEILLI
                     TSRETRRWVIPKGWPMITLAAHDAAAQEAWEEAGVRGEIGASPIGVFRYRKRMKSGPP
                     QRCRVDVFALAVTSEAKDWPEKRQRQRRWVPALEAARMVEEPGLRLLIESFASAREAA
                     "
     gene            complement(842..1840)
                     /locus_tag="PLO65_13050"
     CDS             complement(842..1840)
                     /locus_tag="PLO65_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRD29220.1"
                     /translation="MNVALLILIGLVVIALAFDFLNGLHDAANSIATIVATRVLSPGK
                     AVAWAAFFNFIAFAVFGLHVAKTVGSGIVSPALITDGVIFGALMGAICWNLLTWWLGI
                     PSSSSHALIGGLVGAGIAKGGLHAIVWAGLDKTVLAIVLSPTVGFVLALFLMLIVSWL
                     FVRSTPALADRVFRLLQFVSSSLYSLGHGGNDAQKTMGIIAALLYARGLTGGEFHVPF
                     WVVITCQAAMGLGTLFGGWKIVHTMGSKITRLSPMQGFCAETGGAITLFMATALGVPV
                     STTHTITGAIVGVGAARRVSAVRWGLARSIVWAWVITLPAAAMMGALFFGLSQLFR"
     gene            complement(1850..2494)
                     /locus_tag="PLO65_13055"
     CDS             complement(1850..2494)
                     /locus_tag="PLO65_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HRD29221.1"
                     /translation="MLSWFQAIMPKEDRFFDMFQRHAATLVLGAAALRELLDGGDNVQ
                     ACCERIFRHEDEADDIAREGMLAVRRTFITPFDRSDIQGLFVSLDDSIDQMKKTAKAI
                     VLFEATAFEPQMREMADIIVQCADLTTEMVGLLPRMRQNAGRLNALTERIVKLEDHSD
                     HVCDAGLKALFLKHRDGNAMGYIVGAEIYDHLERVVDRFEDVANGISGILIEHL"
     gene            complement(2594..2670)
                     /locus_tag="PLO65_13060"
     tRNA            complement(2594..2670)
                     /locus_tag="PLO65_13060"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Asp,seq:gtc)
     gene            complement(2733..>3466)
                     /locus_tag="PLO65_13065"
     CDS             complement(2733..>3466)
                     /locus_tag="PLO65_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRD29222.1"
                     /translation="VTVALLWAALALAPGAPLQDPEAVGPARLTPFFRSLVAGFFLLF
                     LASGCVYGLRTGAIRRDSDIVRLASESIAAMAPYIVLAFVASHFIAMFNWSNLGAISA
                     INGADALKRSGLPLALILMGVTVLGAGLDLFIASASAKWAALGPVLVPMLMLLGVSPE
                     MTTAAYRMGDSSLVIMSPMNPYFILVLGFAQRWRPGLGVGGLLSTLLPYALAFFVAGL
                     VVTGLWGALGLATGPAAPFHYLIPS"
BASE COUNT          614 a         1141 c         1232 g          479 t
ORIGIN      
        1 cgtgctggag gccatggcct gcggcctgcc ggtggtgggc gtggacgccg gcggcgtggc
       61 ggaatcggtg gatcacgccg tcgggcagct ggcgtggcgg tcggacagcg cggccctggc
      121 ggaggccgtc gcggccctgt tcgagcggga cctggccttg gtcggtcgga cggcgcggac
      181 ccgggccgag acccgtcacg gctggaaccg ggtgttcgag cagctggcgg cgatctatgc
      241 ccggctgacc tgcgacaccg ccttcgtcac gccgcctccc gcgctgacgc gaagctctcg
      301 atgagcaagc gcagccccgg ttcctcgacc atgcgcgcgg cctccagggc cgggacccag
      361 cggcgttggc gctggcgctt ctcgggccag tccttcgcct cgctggtcac ggcgagggca
      421 aagacatcga cacggcaccg ttgcggcggg ccggacttca tgcgcttgcg atagcggaag
      481 acgccgatcg ggctcgcgcc gatctcgccg cggacgccgg cttcttccca ggcctcctgg
      541 gccgcggcgt cgtgggcggc cagagtgatc atcggccagc ccttggggat cacccagcgg
      601 cgggtctcgc gtgacgtgat cagcaggatt tcgatcccgt ccttgccccg tcgccagggc
      661 agggcggcat actgctggcg gtgtgacgcc caggtcaggg catgaagacg ctcccggacg
      721 cgcccgatca gggaccttcc tcggcgcatg cggacctgga gcctctgcaa caatcgacaa
      781 cccgcgaatc gaaccctgga ccgcgcagga tggttccccc gcccggcggg gtccataggc
      841 gctatcggaa cagctgcgac aggccgaaga acagcgcgcc catcatggcc gcggccggca
      901 gggtgatcac ccacgcccag acgatgctgc gggccaggcc ccagcggacg gcagagactc
      961 gccgggcggc gccgacaccg acgatggcgc cggtgatggt gtgggtggtc gagaccggca
     1021 cgcccagggc ggtggccatg aacagggtga tcgccccccc ggtctcggcg cagaagccct
     1081 gcattggcga cagacgggta atcttgctgc ccatggtgtg gacgatcttc cagccgccga
     1141 acagggtgcc cagccccatc gccgcctggc aggtgatcac cacccagaac ggcacatgga
     1201 actcaccgcc ggtcaggccg cgggcgtaga gcagggcggc gatgatgccc atggtcttct
     1261 gggcgtcgtt gccaccatgg ccgagcgaat agagcgacga ggaaacgaac tgcaggaggc
     1321 gaaatacgcg gtccgccagg gccggggtcg agcggacaaa cagccaggag acgatcagca
     1381 tcaggaacag cgccagcacg aagccgacgg tcggggacag cacgatggcc agcaccgtct
     1441 tgtccagtcc ggcccagacg atggcgtgca gaccgccctt ggcgatcccc gcgccgacca
     1501 ggccgccgat cagcgcgtgg gagctggacg agggaatgcc cagccaccag gtcagcaggt
     1561 tccagcagat ggcccccatc agggcgccga agatcacccc gtcggtgatc agggccggcg
     1621 acacgatgcc gctgcccacg gtcttggcca cgtgcaggcc gaacaccgcg aacgcgatga
     1681 agttgaagaa ggccgcccag gccacggcct tgcctggcga cagcacccgt gtggcgacga
     1741 tggtggcgat ggaattggcc gcgtcgtgca ggccgttgag aaagtcgaag gccagggcga
     1801 tgacgaccag gccgatcagg atcaggaggg cgacgttcac ggcgcggccc tagaggtgtt
     1861 cgatcaggat gccgctgatg ccgttggcga catcctcgaa ccggtcgacc acccgttcga
     1921 gatggtcgta gatctcggcc ccgacgatgt agcccatggc gttgccgtcg cgatgcttga
     1981 gaaagagggc cttgagcccg gcgtcgcaaa catggtcgga gtggtcctcc agcttgacga
     2041 tcctttccgt gagggcgttc agccggccgg cgttctgccg catcctgggc agcaggccga
     2101 ccatctcggt ggtcaggtcg gcgcactgga cgatgatgtc ggccatttcg cgcatctgcg
     2161 gctcgaaggc ggtcgcctcg aacaggacga tcgccttggc ggtcttcttc atctggtcga
     2221 tcgagtcgtc gagcgagacg aacagtccct ggatgtcact gcgatcgaac ggcgtgatga
     2281 aggtgcggcg gacggccagc atgccctcgc gggcgatgtc gtcggcctcg tcctcgtgac
     2341 ggaagatgcg ctcgcagcag gcctggacgt tgtcaccgcc gtccagcagt tcgcgcaggg
     2401 ccgctgcgcc gagcaccagc gtcgccgcat gacgctggaa catgtcgaag aagcggtcct
     2461 ccttcggcat gatcgcctgg aaccagctca gcatggagac tctccgcggg aggtgtcgct
     2521 tggcggccct ataggcgcca gcgccgcgga aggcgagcgc tcttgatctg cggtctgaca
     2581 attttcaagg aaatggcgcg aatgacggga ctcgaacccg cgacctccgg cgtgacaggc
     2641 cggcgctcta accaactgag ctacatccgc aaagacgtcc gcctcgcggc gaggggcgtc
     2701 tgataggtgt gtccctccga cctgtcaaca ggtcaggagg ggatcagata atgaaacggc
     2761 gcggcgggtc cggtggccag gcccagggcg ccccagagcc cggtcacgac gaggcccgcg
     2821 acgaaaaagg ccagggcgta gggcagcagg gtcgacagca ggccgccgac tcccagtccg
     2881 ggccgccagc gctgggcgaa gccgagcacg aggatgaaat aggggttcat cggcgacatg
     2941 atcaccaggc tcgaatcgcc catgcgatag gcggcggtgg tcatctccgg actgaccccc
     3001 agcagcatca gcatcggcac cagcaccggg ccgagcgccg cccacttggc cgaggccgag
     3061 gcgatgaaca ggtcgaggcc tgcgcccagc acggtcacgc ccatcaggat cagcgccagg
     3121 ggcaggcccg agcgcttgag ggcgtcggcg ccgttgatcg ccgagatggc ccccaggttc
     3181 gaccagttga acatggcgat gaagtgcgag gccacgaagg ccagcacgat gtagggcgcc
     3241 atggcggcga tcgactccga cgccagccgc acgatgtcgg agtccctgcg gatcgcgccg
     3301 gtgcgcaggc cgtagacgca gccgctggca aggaacagca ggaagaatcc ggccaccagg
     3361 gagcggaaga acggcgtcag ccgcgccggg ccgacagcct ccggatcctg cagcggcgcg
     3421 ccgggggcca gcgccagggc cgcccacagc agggccacgg tcacgg
//
LOCUS       DAORNX010000336         3463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20523_length_3463_cov_7.259390, whole genome shotgun sequence.
ACCESSION   DAORNX010000336 DAORNX010000000
VERSION     DAORNX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20523_length_3463_cov_7.259390"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLO65_13070"
     CDS             <1..372
                     /locus_tag="PLO65_13070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD29223.1"
                     /translation="LWLAPGAEADSDAIIALVGEAVPEERHDVLGELGELMRQFHPKE
                     PHWYLSMIGVDPSRQGQGLGAALLKAGLARCDADGLPAYLESSSPKNVPLYERHGFEV
                     IGLIKPGDHPGLIPMYRPARG"
     gene            complement(453..1208)
                     /locus_tag="PLO65_13075"
     CDS             complement(453..1208)
                     /locus_tag="PLO65_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29224.1"
                     /translation="MERLPKAVAGALFGAAGLFLTTSVLKVLIDGGVPSVAAWMIVIL
                     ITTFIILIFETYGDNLKALGPIRALYHRYKYEGIWLQNVQVTERPYAISVIYYDWSER
                     KWIYRGTAFDKDMQPAARWKTYGIAFDNRKNRWIFRGTSQLIERAPGGHYHSSPPSEV
                     HCMLKLPDFQKSTITDAYAIDLVAKDGRPHSFYIGLYRCRRDIVQSLLADGIKYPKHI
                     EEISFDLGGKIIKEHIDRAVLPPNAWQMFKSDL"
     gene            complement(1260..1982)
                     /locus_tag="PLO65_13080"
     CDS             complement(1260..1982)
                     /locus_tag="PLO65_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRD29225.1"
                     /translation="MSDDEPPKRRMVRLPTAGNEGVGRGKPARLKTAKGRRPSSQAWL
                     ERQINDPFSAEARALGYRSRAAFKFTEIDDRFHLVKPGSKIVDLGCAPGGWVQMALKR
                     GAGKIVGIDLLPVDPLPPAEMIQMDFTDPAAGPKLIEMLGGPPDLVLSDMAPNTVGHR
                     ETDHLRIVGLIELAAAFAIEVLKPGGAFVAKAFQGGETAQIIRDLKKRFDKVQHVKPK
                     ASRTDSSEVFLVATGFRGNTNS"
     gene            complement(1979..3094)
                     /locus_tag="PLO65_13085"
     CDS             complement(1979..3094)
                     /locus_tag="PLO65_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRD29226.1"
                     /translation="MSEAPSALGGPRGDRRGPNGGPPPAYAALDLGTNNCRLLVATPT
                     HGGFRVVEAYSRIVRLGEGLSLTGRMSDEAMDRAVAALTVCAEKVRRRRCVRIKAIAT
                     QACRQAENGEEFIGRVASETGLRLQIITPKEEAQLSVAGCVSLLDRQAEAALVVDVGG
                     GSTELSWVDLKGSGLDARPRQFAPWRLPIKAWLSVPIGVVTLAERFPEAGDDVEGWFR
                     AMVEAVKERIAAFPHAEPFRQVFETGGAHMVGTSGAITSLAGLHLDLPRYDRNQVDGL
                     WMTRGECEAITGRLLAWTPAERAAQPCIGPDRADLVLAGAAILQAVQELWPCERVRVA
                     DRGLREGLLMSLMSDEQPRRRRRKRGGAGRKPVQASA"
     gene            3256..>3463
                     /gene="hspQ"
                     /locus_tag="PLO65_13090"
     CDS             3256..>3463
                     /gene="hspQ"
                     /locus_tag="PLO65_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HRD29227.1"
                     /translation="MNARLAKFAIGQVVRHRIFPFRGVIFDVDPEFANTEEWWESIPE
                     DIRPRKDQPFYHLLAENNENTYVAY"
BASE COUNT          619 a         1132 c         1066 g          646 t
ORIGIN      
        1 ctgtggctcg cgccgggcgc agaggcggac agcgacgcga tcatcgccct ggtcggcgaa
       61 gccgtgcctg aagagcgcca cgacgtgctg ggagagctcg gcgaactgat gcggcagttc
      121 cacccgaagg agccgcactg gtatctgtcg atgatcggcg tcgacccttc gcggcagggc
      181 cagggcctgg gcgcggcgct gctgaaggcc ggcctcgcgc gctgcgacgc cgacggcctg
      241 ccggcgtatc tggagagctc cagcccgaag aatgtcccgc tctacgaacg ccatggcttc
      301 gaggtcatcg gtctgatcaa gccgggcgat cacccggggc tgatcccgat gtaccggccc
      361 gcgcgcggtt aacgtggagt ccggaagcta aaacagtgaa catcggtgca gagcaattcg
      421 gcttaaagcc ataatggtta agaagcacaa acctatagat cagatttgaa catctgccat
      481 gcgtttggcg gaagaacagc gcgatcaata tgttctttta ttatctttcc gccaagatcg
      541 aagctgattt cttcgatgtg ttttgggtat tttataccat cggcaagcaa gctttgaact
      601 atatctcttc gacatctata cagcccgatg tagaatgaat gcggccttcc atccttggcg
      661 accaagtcaa tagcatacgc gtccgtaatt gttgacttct gaaaatctgg caactttaac
      721 atacaatgaa cttcgctcgg cggagatgaa tgatagtgcc ctccaggcgc cctctcaatc
      781 aactgagacg ttcctcgaaa aatccacctg ttctttcgat tgtcaaatgc aatgccataa
      841 gttttccatc tagccgccgg ctgcatatcc ttatcgaatg ccgttccacg atagatccac
      901 tttctctcgc tccagtcata gtatatcacg gatatggcgt acggcctttc tgttacttga
      961 acattttgaa gccatatgcc ctcatatttg tacctgtgat acagggcccg aatgggccct
     1021 agcgccttta gattgtcgcc gtaggtctca aaaattaaga tgatgaaggt cgtaatcaag
     1081 attacgatca tccaggcggc aacagacggc acacctccat ctattaaaac cttgagaacc
     1141 gaggttgtaa ggaatagccc ggcagcccca aagagggccc cggccacagc ttttggcagc
     1201 cgttccacag aatttctccc gcccaactca atttgggcgg atgagtttcc gctcgtcaac
     1261 taagaattgg tattccccct gaaccccgtc gcgacgagga agacctccga gctgtccgtc
     1321 cggctggcct tcggtttcac gtgctggacc ttgtcgaagc gcttcttcag gtcgcggatg
     1381 atctgggccg tctcgccgcc ctggaaggcc ttggccacga aggccccgcc gggcttgagc
     1441 acctcgatgg cgaaagccgc ggccagttcg atcaggccga cgatgcgcag gtggtcggtc
     1501 tcgcggtggc cgaccgtgtt gggggccatg tccgacagca cgaggtccgg cggcccgccg
     1561 agcatctcga tcagcttcgg cccggccgcc gggtcggtga agtccatctg gatcatctcg
     1621 gccggcggca atgggtcgac gggcagcaga tcgattccga cgatcttgcc ggccccgcgc
     1681 ttgagcgcca tctgcaccca gccacccggc gcgcagccca ggtcgacgat cttcgagccg
     1741 ggtttcacca ggtggaaccg gtcgtcgatc tcggtgaact tgaaggccgc gcggctgcga
     1801 tagcccaggg cccgggcctc ggccgagaac gggtcgttga tctgccgctc cagccaggcc
     1861 tgggacgagg gccgccggcc cttggcggtc ttcaggcgcg cgggcttgcc ccggccgacg
     1921 ccttcgttgc cggccgtcgg caatcgcacc atgcggcgtt tggggggctc gtcatcactc
     1981 atgcggaggc ctgtacaggt ttccgccccg cgccgccccg tttgcgtcgc cgccggcgcg
     2041 gctgctcgtc gctcatcagg ctcatcagca gcccctcgcg caggccgcgg tcggccacgc
     2101 gcacccgctc gcacggccaa agctcctgca ccgcctgcag gatggctgcg ccggccagca
     2161 ccagatcggc ccggtcgggc ccgatgcagg gctgggccgc gcgctcggct ggtgtccagg
     2221 ccagcagccg gccggtgatg gcctcgcact cgccacgggt catccacagg ccgtcgacct
     2281 ggttgcgatc atagcgcggc aggtccaggt gcaggccggc caggctggtg atggcgcccg
     2341 aggtgccgac catgtgcgcg ccgccggtct cgaacacctg acggaacggc tcggcgtggg
     2401 gaaaggccgc gatccgttcc ttgaccgcct cgaccatcgc ccggaaccag ccttcgacgt
     2461 cgtcgccggc ctcgggaaaa cgctcggcca gggtgacgac cccgatcggc accgacagcc
     2521 aggccttgat cggcaggcgc cagggcgcga actggcgcgg gcgggcgtcg aggcccgacc
     2581 ccttgagatc gacccacgac agctccgtcg agccgccacc gacatcgacc accagggcgg
     2641 cttcggcctg gcggtcgagc aggctgacgc aaccggcgac cgacagctgg gcctcttcct
     2701 tgggggtgat gatctgcagc cgcaggccgg tctcgctggc cacgcggcca atgaactcct
     2761 cgccgttctc ggcctgtcgg caggcctggg tggcgatggc cttgatgcgc acgcagcgac
     2821 ggcgacgaac cttctcggcg cagacggtca gcgccgccac ggcccggtcc atggcttcgt
     2881 ccgacatgcg accggtcagg ctcaggcctt cgcccagccg gacgatgcgc gaataggcct
     2941 cgaccacgcg gaatccgcca tgggtcgggg tggcgaccag caggcggcag ttgtttgtgc
     3001 caagatccag cgccgcgtag gcgggtggcg ggcccccatt ggggcccctg cgatccccgc
     3061 ggggaccgcc tagcgcactg ggcgcctccg acatggcgtc atacctgtct gcgcaggcgc
     3121 cccatgatcg ggcgcctcgt ttcaggtgtg accttagccg tccgtttcgc ccagcggaag
     3181 gcattgactt gcctaacggc gccgggcgtc tacgttcatc ttccagtcag ggccgctgcg
     3241 ctagattccc gaatcatgaa cgccagactc gccaaattcg ctatcggcca ggtggtcagg
     3301 caccggatct ttccgttccg gggcgtcatc ttcgacgtcg atccggagtt cgccaatacc
     3361 gaggaatggt gggagtcgat ccccgaggac atccgtccgc gcaaggacca gccgttctac
     3421 cacctgctgg ccgagaacaa cgagaacacc tacgtcgcct atg
//
LOCUS       DAORNX010000337         3425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20818_length_3425_cov_8.741840, whole genome shotgun sequence.
ACCESSION   DAORNX010000337 DAORNX010000000
VERSION     DAORNX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20818_length_3425_cov_8.741840"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLO65_13095"
     CDS             <1..440
                     /locus_tag="PLO65_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRD29228.1"
                     /translation="PLGAVALATSELRRNEGSPAARLKTLSYVDNVLARRMAAPAEAL
                     LLNNRGEIACAAAANIFWLRDGRLFTPHRDCGVLDGVMRAQVMAMAEVEEVQAPRAML
                     EGADAIFLTSSLIGLRPVASLDGTALRTHRLVDDLAAALAAVS"
     gene            complement(433..966)
                     /locus_tag="PLO65_13100"
     CDS             complement(433..966)
                     /locus_tag="PLO65_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD29229.1"
                     /translation="MTGFLRRLGRDRRGVSAVEFALIAPAMIACYFGMAEITQALLAE
                     RKAAHAASAVGDLVTQSSSVSSDDITDIFAIARTIMAPYSTTSLKMRVSSLTADSGGT
                     PKVDWSSASGLTALTTGSTVSDVPTGVISAGQSVVMSEVTYSYDSPVDVMMPNAVTFT
                     RKFYLRPRKSDKVAKTS"
     gene            complement(963..1508)
                     /locus_tag="PLO65_13105"
     CDS             complement(963..1508)
                     /locus_tag="PLO65_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD29230.1"
                     /translation="MASPARKPKSLLGRFRRARDGATAVEFAIVAAPFLALMFGILDL
                     GLVFMVSTTLENAVETAARKIRTGELQMSGGTASTFKTAVCDEMTWLGSGCASAVHVD
                     VRTFPTFADVDFTDPTTNGAFDDSKTTFAPGGSESIVLVRVYYEWTLMTPLINTGLKN
                     LSNGNRLISSTATFRNEPYAS"
     gene            complement(1572..2051)
                     /locus_tag="PLO65_13110"
     CDS             complement(1572..2051)
                     /locus_tag="PLO65_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HRD29231.1"
                     /translation="MRRPLALFAAFAVLTTSGGALAQSLPVRIDEAARVALSATARDV
                     IIGNPSVADVTIIDGRNLLVMGKAYGVTNIVVLDARGRTILDRQIVVSASDEGRVSFF
                     RGPDVYNYACSPRCERTPMPGERDSGQGVYSQWSGPYTAYPDRAEAAAAAAKASTPR"
     gene            2346..2510
                     /locus_tag="PLO65_13115"
     CDS             2346..2510
                     /locus_tag="PLO65_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRD29232.1"
                     /translation="MTKFMTRFAKDESGATAIEYGLIVALIAVVIITAVTTLGTKLNT
                     AFTAINDKLT"
     gene            2780..2944
                     /locus_tag="PLO65_13120"
     CDS             2780..2944
                     /locus_tag="PLO65_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRD29233.1"
                     /translation="MSKFMSRFAKDESGATAIEYGLIVALIAVVIITAVTTLGTKLNT
                     AFTAINDKLT"
     gene            3073..>3425
                     /locus_tag="PLO65_13125"
     CDS             3073..>3425
                     /locus_tag="PLO65_13125"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRD29234.1"
                     /translation="MLHLVQTALLFIFPALVIVGAMRDAVSYTIPNWVSIALAVAFLP
                     AALAMGVAPGQFGLACLIGLGALVAGMAMFAVGWIGGGDAKLFAAAGLWMGLGGLLPY
                     LLVTALAGGALAVGLL"
BASE COUNT          558 a         1127 c         1161 g          579 t
ORIGIN      
        1 ggccgctcgg ggcggtcgcc ctggccacca gcgagctgcg ccgcaacgag ggctcgccgg
       61 cggcgcggct gaagaccctg tcctacgtcg acaatgtcct ggcccgccgc atggccgccc
      121 cggccgaggc cctgctgctc aacaaccgcg gcgagatcgc ctgcgcggcg gcggccaata
      181 tcttctggct tcgcgacggc cggctgttca ccccgcaccg cgactgcggc gtgctcgacg
      241 gggtcatgcg ggcccaggtg atggccatgg cggaagtcga ggaggtgcag gcgccgcggg
      301 ccatgctcga gggcgccgac gcgatcttcc tgaccagcag cctgatcggc ctgcggcccg
      361 tggccagcct cgacgggacc gccctgcgca cgcaccggct ggtcgatgac ctggccgcgg
      421 ccctggcggc ggtcagctag tcttggccac cttgtcggac tttcgcggcc gcaggtagaa
      481 cttgcgggtg aaggtgacgg cgttgggcat catcacgtcg accggcgagt cgtagctgta
      541 ggtcacctcc gacatgacca cgctctgccc cgcgctgatc acgccggtgg gcacgtcgga
      601 cacggtcgag ccggtggtca gggcggtcag gcccgaggcg ctgctccagt cgaccttcgg
      661 cgtaccgccg gagtcggccg tcaggctgct gaccctcatc ttcagcgagg tcgtcgaata
      721 gggcgccatg atcgtccgcg cgatcgcgaa gatgtcggtg atgtcgtcgc tgctgacgct
      781 cgaggactgc gtcaccaggt cgccgacggc ggaggcggcg tgggccgcct tgcgttctgc
      841 cagcagggcc tgggtgatct cggccatgcc gaagtagcag gcgatcatcg ccggggcgat
      901 cagcgcgaac tcgaccgcgg agacaccgcg gcggtcgcgg cccagccttc tgagaaatcc
      961 ggtcatgatg cgtagggctc gttgcggaag gtcgccgtcg aggagatcag ccggttgccg
     1021 ttggaaaggt tcttcaggcc ggtgttgatc agcggcgtca tcagggtcca ctcgtagtag
     1081 acccggacca ggacgatgga ttccgaacca cccggcgcga aggtggtctt gctgtcgtcg
     1141 aaggccccgt tggtggtcgg atcggtaaaa tcgacatcgg cgaaggtcgg gaaggtgcgc
     1201 acgtcgacat gaacggccga ggcgcagccg gagccgagcc aggtcatctc gtcgcagacg
     1261 gcggtcttga acgtcgaggc cgtgcccccg ctcatctgca gctcgccggt gcggatcttg
     1321 cgggcggcgg tctcgacggc gttttcgagg gtcgtcgaga ccatgaacac caggcccaga
     1381 tcgagaatgc cgaacatcag cgccaggaac ggggccgcca cgatggcgaa ctcgaccgcc
     1441 gtcgcgccat cgcgcgcgcg ccggaagcgg ccgagcaggg atttcggttt gcgggccgga
     1501 ctggccattg tcgggaacgc ccttcccatc ggaatggaga ggacaatagc gctgcgtccc
     1561 taaccgtcgg tttaccgggg cgtcgaggcc ttcgccgccg ccgccgcggc ctcggcccgg
     1621 tcgggatagg cggtgtaggg gcccgaccac tggctgtaga cgccctggcc ggagtcgcgt
     1681 tcacctggca tcggcgtgcg ttcgcagcgc ggcgagcagg cgtagttgta gacgtccggg
     1741 ccgcggaaga acgacacccg gccctcgtcc gaggccgaga cgacgatctg gcggtccaga
     1801 atcgtgcggc cgcgggcgtc cagcacgacg atgttggtca ccccgtaggc cttgcccatg
     1861 accagcaggt tgcgaccgtc gatgatcgtc acgtcagcca ccgaggggtt gccgatgatc
     1921 acgtcgcggg ccgtcgccga cagggccacc cgcgccgcct cgtcgatgcg caccggcagg
     1981 ctctgggcca gggcgccgcc gcttgtggtc aggaccgcga aggcggcgaa gagggccagg
     2041 ggacggcgca tgggaaacgg aaacctcgtc tgcggacgcc acagactcgg ccggaatggt
     2101 caacaaagac tgaaaccggc ccgcgcgcgg gcgcgcggga atcttataga gtcccccgcc
     2161 ccttccggag gccatggtta agggatggta atagtaaact taagtaaacc atagaaaaaa
     2221 ttcgggaata ttctgctgtt tactggccat taaacgcgct ggcctagggt ggccttgttt
     2281 tcgggggcga gcaggccaag cggcttgatc ccgctaccgc cagcttgctg acaggagatc
     2341 aagcaatgac caagttcatg actcgcttcg ccaaggatga atccggcgcc acggccatcg
     2401 aatacggcct gatcgtcgcc ctcatcgccg tcgtcatcat caccgccgtg acgaccctgg
     2461 gcacgaagct caacaccgcc ttcacggcga tcaacgacaa gctgacctaa acctcgtcag
     2521 cttgaccgtc gaagacagta ggccgcggag agatccgcgg cctttctgct atctggccgc
     2581 tgccccgacc cccgatttga gccacaatta tttgtcgccg cgccgatctg gcccgctcct
     2641 cgtcaatgat cgattcacgc tcgggggagc atattcacat tcgaagaccg ggcgagttcc
     2701 tcaggttact gccgtccgat ccggaaggtt tcagatcaac tgaactggtc tcggccagtt
     2761 cttggaaggg agatacccaa tgtcgaagtt catgtcgcgt ttcgccaagg acgagtccgg
     2821 cgccacggcc atcgagtacg gcctgatcgt cgcgctcatc gccgtcgtca tcatcaccgc
     2881 cgtgacgacc ctgggcacga agctcaacac cgccttcacg gcgatcaacg acaagctgac
     2941 ctgacgtcgg cgaccggctc gccgaagcgg ggccgcggag caatccgcgg ccctttgtct
     3001 tttttggcgc gcgtcaacca tcggttcacc gaggcgcgcg aaggtcgggc aagagctcac
     3061 ctgcggaatc ccatgctgca cctggtccag accgcgctgc tgttcatctt cccggccctg
     3121 gtcatcgtcg gggcgatgcg cgacgccgtc agctacacca ttcccaactg ggtctcgatc
     3181 gcgctcgccg tcgccttcct gccggcggcc ctggcgatgg gcgtcgcgcc tggccagttc
     3241 ggactggcct gcctgatcgg cctgggcgcc ctggtggccg gcatggccat gttcgccgtc
     3301 ggctggatcg gcggcggcga cgccaagctg ttcgcggcgg cgggcctgtg gatgggcctg
     3361 ggcggcctgc ttccctatct gctggtcacc gcgctggccg gcggggccct ggcggtcggg
     3421 ctgct
//
LOCUS       DAORNX010000338         3413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20898_length_3413_cov_4.230197, whole genome shotgun sequence.
ACCESSION   DAORNX010000338 DAORNX010000000
VERSION     DAORNX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20898_length_3413_cov_4.230197"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLO65_13130"
     CDS             complement(<1..706)
                     /locus_tag="PLO65_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD29235.1"
                     /translation="MRLLIPIIATLLLAACAPAPTSGATTPPLDAALLDQEIAVIADR
                     ARPATIEVAVQNLEGGEMWAWNGDKAFPMQSVFKAPLGAAVLAEVDAGRLSLDEVVTL
                     AEPDISPAHSKIADAWPGRTTYTVRELLVWAVGDSDNTAADVLMRRIGGPGAVTAWLR
                     AKGIKEIRVDRYERELQPEISGMASFRIAWKGWPAFAAARDRVPEADRRAATARYLAD
                     PRDTMTATGALTFLRML"
     gene            824..1612
                     /locus_tag="PLO65_13135"
     CDS             824..1612
                     /locus_tag="PLO65_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD29236.1"
                     /translation="MAVSAPVVILNEPQLAENIGSVARVMANFGLSDLRLVRPRDGWP
                     QDRAWASASGADWPLDNARVFDRLEEAVADLRLVYATTARSRGTQLPVLTPREAAANL
                     HQADAQGQAVGILFGGERAGLETDDIALCQAIVTIPVDERFRSLNLAQAVALNAYEWR
                     LGVADRPREAFAVNVDEPADMATLVGLYEHLEGELDAAGFFHPPEKRPSMVRNLRISL
                     GRARLTDQEVRTWRGVVTALSKGRGRVLEKLARQRTSAKDETKG"
     gene            1633..>2614
                     /locus_tag="PLO65_13140"
     CDS             1633..>2614
                     /locus_tag="PLO65_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRD29237.1"
                     /translation="MDRRTLLAAAGAIGIGAAFPALAADDEAAAARDAWFYALPLIEM
                     ATTRARHLANGGAINRFNPSRRLLDQDDRAVTTPNNDTLYSQAWLDLTRGPVTLTVPD
                     MGDRYWSIAVMDMFTNNNVVLGGRTVGSGPATFTFVGPGEDAYPADAVPVATPHAWVL
                     ARVLVDGPDEVPTVLKLLQAIGLSGPTDTPPTPAYAGRSADADAYFAAARALLAADPA
                     PMTDLRHLRRTAAILGPNAGPLSAAARTGVTQAQGLAKAAFARQAYVQGWSYPRPNLG
                     DYGQDYLYRAAVALAGLGALPPAEAMYMRAEGTGRGLFDDDAVRRLTLPAK"
     assembly_gap    2615..2684
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2685..2904
                     /locus_tag="PLO65_13145"
     CDS             <2685..2904
                     /locus_tag="PLO65_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRD29238.1"
                     /translation="NRFAIGDRTPGIRRNADGSLDLWISRADPGGERSANWLPAPASG
                     PYALFFRAYLPGPELLDGRWRLPAIVAA"
     assembly_gap    3021..3080
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3081..>3413
                     /locus_tag="PLO65_13150"
     CDS             <3081..>3413
                     /locus_tag="PLO65_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRD29239.1"
                     /translation="DTLYATAQIDLSAGPVTLTVPPSGDRYVSLQLMDAWSNSFCLLG
                     TRTTGNGGGTFTLVGPEEAASGRNVIRAPSRHVWALARILVDGPHDLDAARVVQGGFS
                     AQGPDVPAP"
BASE COUNT          452 a         1228 c         1142 g          461 t
ORIGIN      
        1 acagcatgcg caggaaggtc agggcgccgg tggcggtcat ggtgtcgcgc gggtcggcga
       61 ggtagcgcgc cgtggccgcg cgtcggtcgg cctcgggcac gcggtcgcgg gccgcggcga
      121 aggccggcca gcccttccag gcgatgcgga acgaggccat gccgctgatt tcgggctgca
      181 gctcccgctc gtaccggtct acgcggatct ccttgattcc cttggcgcgc aaccaggccg
      241 tcaccgcccc ggggccgccg atgcgccgca tcaacacgtc ggcggcggta ttgtcgctgt
      301 cgcccacggc ccacaccagc agctcgcgca ccgtgtaggt tgtccgcccg ggccaggcgt
      361 cggcgatctt gctatgggcg ggcgagatgt ctggttcggc cagggtgacg acctcgtcca
      421 gcgacaggcg gccggcgtcg acctcggcca gcaccgccgc gccgagcggg gccttgaaca
      481 cgctctgcat cggaaaggcc ttgtcgccgt tccaggccca catctcgccg ccctcgaggt
      541 tctgcaccgc cacctcgatg gtggccggcc gggcgcgatc ggcgatgacg gcgatctcct
      601 ggtcgagcag ggcggcgtcg agaggcggcg ttgtggcccc gctcgtcggc gccggcgcgc
      661 aggcggccag cagcagcgtg gcgatgatcg gaatgagcag gcgcatggct gaccttcata
      721 acgcgttcgc gacgccagcg ggcggtcagg gcgtatcttt tctgctattc gggcggcccg
      781 tccgcctgaa acaggcccct cgcgaccgtt cgaggagccg gagatcgccg tgtccgcacc
      841 cgtcgtcatc ctcaatgaac cccagctggc cgagaacatc ggatcggtcg cccgggtgat
      901 ggccaacttc gggctgagcg atctgcgcct ggtgcggccg cgggacggct ggccgcagga
      961 ccgggcctgg gcctcggcct cgggggccga ctggccgctc gacaacgccc gggtgttcga
     1021 ccggctcgag gaggcggtgg ccgacctgcg gctggtctac gccaccacgg cgcggtcgcg
     1081 ggggacgcag ctgcccgtcc tgaccccgcg cgaggcggcg gcgaaccttc atcaggccga
     1141 cgcccagggc caggccgtcg gaatactgtt cggcggcgag cgggccgggc tagagaccga
     1201 cgacatcgcc ctgtgtcagg ccatcgtcac catccccgtc gacgagcggt tccgctcgct
     1261 gaacctcgcc caggcggtcg ccctcaacgc ctacgaatgg cggctcggcg tcgccgaccg
     1321 gccgcgcgag gcctttgcgg tcaatgtcga tgaaccggcc gacatggcca ccctggtcgg
     1381 cctgtacgag catctcgagg gggaactcga cgccgccggc ttctttcatc cgccggaaaa
     1441 acggccctcg atggtgcgca acctgcgcat ctcgctcggg cgggcccgac tgaccgacca
     1501 ggaggtgcgg acctggcgcg gcgtggtcac cgccctgtcg aaagggcgcg gccgggtgct
     1561 cgagaagctt gcgcgccagc ggacttccgc taaggatgag accaaagggt aacaaatttc
     1621 tcggagggac ggatggaccg caggaccctg ctggcggcgg ccggcgcgat cggcattggc
     1681 gcggcgtttc ccgccttggc cgccgacgac gaggcggccg ccgcccgcga cgcctggttc
     1741 tatgccctgc cgttgatcga gatggccacc acccgcgccc ggcacctggc caacggcggg
     1801 gccatcaacc ggttcaaccc cagccggcgc ctgctcgacc aggacgaccg ggcggtgacc
     1861 acgcccaaca acgacaccct ctattcccag gcctggctgg acctgaccag gggtccggtg
     1921 accctgaccg tccccgacat gggcgaccgc tactggtcga tcgcggtcat ggacatgttc
     1981 accaacaaca acgtcgtgct cggcggccgc acggtcggat cggggccggc gaccttcacc
     2041 ttcgtcggtc cgggcgagga tgcctatccg gccgacgccg tcccggtggc cacgccccac
     2101 gcctgggtgc tggcgcgcgt gctggtcgac ggaccggacg aggtcccgac cgtcctcaag
     2161 ctgttgcagg ccatcggcct ttcaggtccc accgataccc cgccgacacc ggcctacgcc
     2221 ggccggtcgg ccgatgccga cgcctatttc gccgccgcca gagccctgct cgccgcagac
     2281 cccgcgccga tgaccgacct gcgccatctg cgccggacgg ccgccatcct cggccccaac
     2341 gccggaccgc tgtccgccgc ggcgaggacc ggcgtgaccc aggcccaggg cctggccaag
     2401 gccgcattcg cccgccaggc ctacgtccag ggctggagct atccgcggcc caatctcggc
     2461 gactacggcc aggactacct ctaccgcgcc gccgtcgccc tggccgggct gggcgccctg
     2521 ccgccggccg aagccatgta catgcgcgcg gaaggcacgg gcaggggcct gttcgacgac
     2581 gacgcggttc gccggctgac cctgccggcg aaggnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaaccg cttcgccatc
     2701 ggcgaccgta cgccggggat ccggcgaaat gccgacggct cgctcgacct gtggatcagc
     2761 cgcgccgacc ccggtggcga acgcagcgcc aactggctgc cggcgccggc gagcggcccc
     2821 tacgccctgt tcttccgggc ctacctgccg ggcccggagc tgctcgacgg ccgctggcgc
     2881 ctgcccgcca tcgtggcggc ctgagccatg accgacctcc gcaccgccgc ccgcgacgcc
     2941 tggctctaca ccctgccgct gatcgagatc gccgcggtgc gcagccgcgg cctcaccgtc
     3001 ggctcggcga tgaacgcctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn cgacaccctc tacgccacgg cgcagatcga cctgtcggcc
     3121 gggccggtga ccctgaccgt gccgccgtcg ggcgaccgct acgtctcgct gcagctgatg
     3181 gacgcctgga gcaacagctt ctgcctgctc ggcacccgca cgaccggcaa tggcggcggg
     3241 accttcaccc tggttggccc ggaggaggcg gcatcggggc gcaacgtgat ccgcgcgccg
     3301 agccggcatg tctgggccct ggcgcggatt ctcgtcgacg ggccgcacga cctggacgcg
     3361 gcgcgggtgg tgcagggcgg cttctccgcc cagggacccg acgtgccggc ccc
//
LOCUS       DAORNX010000339         3407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_20947_length_3407_cov_7.694809, whole genome shotgun sequence.
ACCESSION   DAORNX010000339 DAORNX010000000
VERSION     DAORNX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20947_length_3407_cov_7.694809"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLO65_13155"
     CDS             <1..252
                     /locus_tag="PLO65_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD29240.1"
                     /translation="EGPRRAGDLARAAGLTAPAMSRHLRTLRQSGLVEETSPDFDTRV
                     RVYQLRPEPMVHLLRWLEETERMWTAQLAAFKAHLETNG"
     gene            252..713
                     /locus_tag="PLO65_13160"
     CDS             252..713
                     /locus_tag="PLO65_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD29241.1"
                     /translation="MASRVVVSLRVPATPRRAFEAFTAEIGQWWTPNGLFAFTPRAPG
                     VLSFDGRERLIETREGGKVFEIGKVTVWEPGARLVVGWRQASFTPDMATEVEVTFEAV
                     GEETRVTVSHVGWDSVPQAHVAKHGFPETVFLARHGEWWRALLERMREHLG"
     gene            835..1179
                     /locus_tag="PLO65_13165"
     CDS             835..1179
                     /locus_tag="PLO65_13165"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRD29242.1"
                     /translation="MTQADIRAIALSLPEAEEQPHFDRASFRVRGKIFVTLPPVLDDG
                     VHRVVLKLPELVKESLQQTDADAVLSLGNWDKGGWTQLDIGRMDDARLADLVRLAWRQ
                     VAPKRLSAGERP"
     gene            1176..2483
                     /locus_tag="PLO65_13170"
     CDS             1176..2483
                     /locus_tag="PLO65_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRD29243.1"
                     /translation="MTDQQHTAAPGGRRAAFGFIFALAVMNAISFGIMIPILPNLIKQ
                     FTSGDTAAASVWNVVFMVTWGLMQFVIGPILGMYSDRYGRRPVLLLSLLGLAVDFLFM
                     ALAPSLVWLLVGRILNGMTAASFSTANAYVADVTPPEQRAKVFGWMGSAFSFGFLVGP
                     VLGGYLGKISLTMADVTVAGLTIDMALRLPFFVAAGLTLVNVLYGLFVLPESLPVEKR
                     AVRFEWRKANPVGSLRLLRSHPDLLGLASVLFLFQLAHMVLPSVFVLYTGYRYGWTPD
                     FMGLTMGLTGILGIGVSIFLVQPVVRRLGEKGALLVGACFGALGFAIYGFAPTTALYL
                     VGTPVFALIGLVMPGLMGLMSRRVAPQEQGQLQGANQSVQGISSILGPLMFGLIFAWS
                     VHNDRTLHLPGLAIWVASGLMVVAFLIGLTVARPPRIAEPLPA"
     gene            2559..>3407
                     /locus_tag="PLO65_13175"
     CDS             2559..>3407
                     /locus_tag="PLO65_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD29244.1"
                     /translation="MRAVKLLIPTLALLAACSDPASKSQAQDVGLVQPTKAPPASVES
                     MKTSFAPVVKKAAPAVVNVSSRRVVRQRVDPFWDLFTGGGVPQERIAQSLGSGAIVRA
                     DGVILTNNHNIDGADEIVVQLADRREFPARVLLADARADLAVLKIDTGGERLPVIAID
                     DQETLEVGDLVLAIGNPFGVGQTVTNGIVSATARSNVGITDYSFFIQTDAAINPGNSG
                     GPLVDMDGDLVGINTAILSRSGQSSGVGFAIPAAMARQVVSTALGGGHVVIRPWLGAR
                     TQAVTTD"
BASE COUNT          479 a         1170 c         1178 g          580 t
ORIGIN      
        1 gaggggccgc gccgcgccgg cgatctggcg cgggccgccg gcctgaccgc cccggccatg
       61 agccggcacc tgcgcaccct gcgccagtcg ggactggtcg aggaaacctc gccggacttc
      121 gacacccgcg tgcgcgtcta tcagctgagg cccgagccca tggtgcacct gctgcgctgg
      181 ctggaagaga ccgagcggat gtggacggcc cagctggcgg ccttcaaggc ccatctggag
      241 accaacggct gatggcctcg cgggtcgtcg tctcgctgcg cgtgccggcc acgccgcggc
      301 gcgccttcga ggccttcacg gccgaaatcg gacagtggtg gacgcccaac ggcctgttcg
      361 ccttcacgcc ccgggcgccc ggcgtcctgt cgttcgacgg ccgcgagcgg ctgatcgaga
      421 cgcgcgaggg cggcaaggtg ttcgagatcg gcaaggtcac ggtctgggag cccggcgcgc
      481 gcctggtggt cggctggcgc caggccagtt tcaccccgga catggccacc gaggtcgagg
      541 tgaccttcga agcggtcggc gaggagaccc gcgtcacggt cagccacgtc ggctgggaca
      601 gcgtgccgca ggcccatgtg gccaaacacg gcttccccga aacggtcttc ctggcgcggc
      661 atggcgagtg gtggcgggcg ctgctggagc ggatgaggga acatctggga tgaggcgctg
      721 tgacatcgtg cgtggttcga cacgcgctct tcgagcgctg ctcaccatga cgttgtcagt
      781 tgtacgtcat cctgagcagg ccgcgcagcg gccgtgtcga aggacgcaag aacgatgacc
      841 caggccgaca tccgcgccat cgccctcagc ctgcccgaag ccgaggaaca gccgcacttc
      901 gaccgggcat cattccgggt gaggggaaag atcttcgtca ccctgccgcc ggtgctcgac
      961 gacggcgttc accgggtcgt cctcaagctg cccgagctgg tcaaggagtc gctgcagcag
     1021 acggacgccg acgccgtcct gtcgctcgga aactgggaca agggcggctg gacgcaactc
     1081 gatatcgggc gcatggacga cgccagactg gccgatctgg tgcgcctcgc ctggcgtcag
     1141 gtcgcgccga agcgactgag cgctggggag cgaccatgac cgatcaacag cacacggccg
     1201 cgcccggcgg ccggcgcgcg gccttcggct tcatcttcgc cctggcggtg atgaacgcga
     1261 tctcgttcgg catcatgatc ccgatcctgc cgaacctgat caagcagttc accagcggcg
     1321 acaccgcggc ggcctcggtg tggaacgtgg tcttcatggt cacctggggg ctgatgcagt
     1381 tcgtcatcgg cccgatcctg ggcatgtatt cggaccgcta tggccgccgt ccggtgttgc
     1441 tgctgtcgtt gctgggtctc gcggtggact tcctgttcat ggccctggct ccgagcctgg
     1501 tctggttgct ggtcgggcgg atcctcaacg gcatgaccgc cgccagcttc tcgacggcca
     1561 acgcctatgt cgccgatgtc accccgcccg agcagcgggc caaggtgttc ggctggatgg
     1621 gctcggcgtt cagcttcggc ttcctcgtcg ggccagtgct gggcggctac ctgggcaaga
     1681 tcagcttgac catggccgac gtgacggtgg ccggcctgac catcgacatg gcccttcgcc
     1741 tgccgttctt cgtcgcggcc ggcctgaccc tggtcaacgt gctctacggc ctgttcgtgc
     1801 tgcccgagtc cttgcccgtc gagaaacggg ccgtgcggtt cgagtggcgc aaggccaatc
     1861 cggtcgggtc gctcaggctg ctgcggtcgc accccgacct gctgggcctg gcgtcggtgc
     1921 tgttcctgtt ccagctggcg cacatggtgc tcccgagcgt cttcgtgctc tacacgggct
     1981 accgctacgg ctggacgccg gacttcatgg gcctgaccat gggcctgacc ggcattctcg
     2041 gcatcggcgt gtcgatcttc ctggtccagc cggtggtccg gcggctcggc gagaagggcg
     2101 cgctgctggt cggcgcctgt ttcggcgccc tggggttcgc catctacggc ttcgccccga
     2161 ccacggccct gtacctggtc ggcacgccgg tgttcgccct gatcgggctg gtcatgccag
     2221 gactgatggg cctgatgagc cgccgcgtgg cgccgcagga gcagggccag ctgcagggcg
     2281 cgaaccagag cgtgcagggc atctcctcga tcctcggacc gctgatgttc ggcctgatct
     2341 tcgcctggtc ggtgcacaac gaccggaccc tgcatctgcc tggcctggcc atctgggtgg
     2401 cgtcgggact gatggtcgtc gctttcctga tcgggctgac ggtggcgcgg ccgccgagga
     2461 tcgcggaacc cctgccggcc tgaggtcttt catcgccatc attgcgcccg aaatcttcgc
     2521 tgcattgcgg ccggggcaac ctcgatctga aagacctcat gcgcgccgtg aaactgctga
     2581 ttcccaccct ggccctgctg gccgcctgtt ccgacccggc ttcgaaatcc caggcccagg
     2641 acgtcggtct ggtccagccg accaaggctc cgccggccag cgtcgagagc atgaagacct
     2701 ccttcgcccc ggtggtgaag aaggccgccc cggcggtggt caacgtctcc agccggcggg
     2761 tcgtgcgcca gcgggtcgac ccgttctggg acctgttcac gggcggcggg gtgccgcagg
     2821 agcggatcgc ccagtcgctc ggctcggggg ccatcgtccg cgccgacggg gtgatcctga
     2881 ccaacaacca caacatcgac ggcgccgacg agatcgtggt ccagctcgcc gaccggcggg
     2941 agtttccggc cagggtgctg ctggccgacg cgcgggccga cctggcggtg ctgaagatcg
     3001 acaccggcgg cgagcgcctg ccggtgatcg ccatcgacga tcaggagacg cttgaggtcg
     3061 gtgacctggt gctggccatc ggcaacccgt tcggggtcgg ccagacggtg accaatggca
     3121 tcgtctcggc cacggcccgc tcgaacgtcg gcatcaccga ctacagcttc ttcatccaga
     3181 ccgacgcggc catcaatccg ggcaacagcg gcggccccct ggtcgacatg gacggcgacc
     3241 tggtcggcat caacaccgcc atcctgtcgc ggtcgggcca gtcgtcgggc gtcggcttcg
     3301 ccattccggc ggccatggcc cggcaggtgg tcagcaccgc gctcggcggc ggccatgtgg
     3361 tcatccgccc ctggctcggc gcccgcaccc aggcggtgac gaccgac
//
LOCUS       DAORNX010000340         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21234_length_3369_cov_6.166264, whole genome shotgun sequence.
ACCESSION   DAORNX010000340 DAORNX010000000
VERSION     DAORNX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21234_length_3369_cov_6.166264"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLO65_13180"
     CDS             <1..382
                     /locus_tag="PLO65_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD29245.1"
                     /translation="SPFADAFDAAALLQRAGFALPVADVDSVTVRYASPLALLRDLRA
                     MGETNVLRERPRRPLTRRVLMRALELYAQRFSGPDGKVVATFEILTVTGWAPHESQQK
                     PLKPGSAKMRLADALGTVERKAPR"
     gene            complement(462..626)
                     /locus_tag="PLO65_13185"
     CDS             complement(462..626)
                     /locus_tag="PLO65_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRD29246.1"
                     /translation="MKRFWRDQSGATAIEYGLIASLVFLVIVTSVTVFGGKTSDKMQY
                     VSDAIAAAIN"
     gene            complement(623..1042)
                     /locus_tag="PLO65_13190"
     CDS             complement(623..1042)
                     /locus_tag="PLO65_13190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRD29247.1"
                     /translation="MSEPLPAKPILLVAAAALIDVDGRVLICQRPRGKQLAGLWEFPG
                     GKVEAGETPETCLIRELKEELGIGVTQACLAPFVFASHGYDSFHLLMPLYLCRRWDGF
                     VTAHEHEALAWVKPDKLSDYPMPPADAPLVAFLRDLI"
     gene            complement(1098..1967)
                     /locus_tag="PLO65_13195"
     CDS             complement(1098..1967)
                     /locus_tag="PLO65_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRD29248.1"
                     /translation="MKPTVAILGANGVYGRHLTPRLVAAGYRVRALVRRVEAAGVARA
                     CGAEVRAADIFDPDSLRRGLDGCDTCINLATSLPGPSGRGDYDVNDRVRREGVPLLLE
                     ACRQTGVTRLLQQSIAMVGAAGEALVDEDTVRRPDSDDVSARAIAATLDMEEAVRASG
                     LDWVILRGGLFYGPGTGFDDDWFARAAAGRLRLPGDGSDFVSLVHIADMAAATVATLA
                     AWPSRQTLIIADDRPARWREVFDHVAEVAGADPPSPGGRAGFPAFRVSNSRARQVLGW
                     SPAYPDYRAGLAR"
     gene            complement(2069..>3369)
                     /gene="argJ"
                     /locus_tag="PLO65_13200"
     CDS             complement(2069..>3369)
                     /gene="argJ"
                     /locus_tag="PLO65_13200"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRD29249.1"
                     /translation="ATTKRPANAAAAEPAGAPGKPGLAVSPLAVPFPKMPPIGGVQIG
                     IGRAGFYKHERPDLLVMRFAKGTSCGGVFTRHGVGSAPVDWCKKQLENSGGEGVQALV
                     VNAGCANSFTGKPGADAVRRVAGAVAKRFDCRQRDVMMASTGVIGVVLDDGKITSRLP
                     EVDGRLTDDGWADAARAIMTTDTFAKGATATAKIDGRTVRINGIAKGSGMIAPDMATM
                     LAFVATDAAIAPKALQTLVSLYTRTTFNAVTVDGDRSTNDTLLLFATGASGAPRISRA
                     GDHRLADFREKLEALLLNLALQLVRDGEGATKFVKINVTGAESAASARKIARTVAESP
                     LVKTAFAGEDANWGRIVMAVGRADEPVNREKMSVAFGKLVAAHDGMVSPTYSEARMSA
                     YMKKPELEVSIDVGVGRGVATVWTCDLTKQYVAINGDYRS"
BASE COUNT          474 a         1193 c         1209 g          493 t
ORIGIN      
        1 ctcgccgttc gccgacgcct tcgacgccgc cgcgctgctg cagcgggccg gcttcgccct
       61 gccggtggcc gacgtcgaca gcgtgaccgt gcgctacgcc agccctctgg ccctgctgcg
      121 cgacctgcgg gcgatgggcg agaccaatgt cctgcgcgag cggccgcgcc ggccgctgac
      181 ccgccgggtc ctgatgcggg cgctggagct ctatgcccag cggttcagcg gcccggacgg
      241 caaggtcgtc gcgacgttcg agatcctcac cgtcaccggc tgggccccgc acgagagcca
      301 gcagaagccg ctcaaaccgg gctcggcgaa gatgcgtctg gccgacgccc tggggacggt
      361 ggagcggaag gcgccgcgct agggcgggac ggggatgggg acagggtaag ctgtccccct
      421 gccggcgcct ggcccgcgaa tgcgcaagct gtcgccgaag gctagttgat cgcggcggcg
      481 atggcgtcgc tgacgtactg catcttgtcg gaggtcttgc cgccgaacac cgtcaccgag
      541 gtgacgatga ccaggaacac cagcgaggcg atcaggccgt attcgatcgc cgtggcgccg
      601 gactggtcgc gccagaagcg cttcagatga gatcgcgcag aaaggcgacc agcggcgcgt
      661 cggccggcgg catcggatag tcggagagct tgtcgggctt cacccacgcc agcgcctcat
      721 gctcgtgcgc cgtgacaaag ccgtcccagc gcctgcacag gtagagtggc atgagcaggt
      781 gaaacgaatc gtaaccgtgg ctggcgaaaa cgaacggggc caggcaggcc tgggtcacgc
      841 cgatgcccag ttcttccttc agttcgcgga tcaggcaggt ctcgggcgtc tcgccggcct
      901 cgaccttgcc gcccggaaac tcccacaggc cggccagctg tttgccccgc ggccgctggc
      961 agatcagcac ccggccatcg acgtcgatca gcgcggcggc ggcgaccagc aggatcggct
     1021 tggcggggag gggctcggac atggcgatcg actagcagtc gtcctcgccc tgcgccagcc
     1081 aaagcccggc cgctggccta tcgcgccagc ccggcccggt agtccgggta ggccggagac
     1141 cagccaagga cctgccgggc ccgcgaattg ctgacccgga aggcagggaa cccggcgcgg
     1201 ccgcccggcg agggcggatc ggcgccggcc acctccgcga catggtcgaa gacctcgcgc
     1261 cagcgcgccg gccggtcgtc ggcgatgatc agggtctgtc gcgagggcca ggccgccagt
     1321 gtggcgacgg tggcggcggc catgtcggcg atgtggacca gcgacacgaa gtccgagccg
     1381 tctcccggca accgcagccg tccggccgcg gcgcgcgcga accagtcgtc gtcgaacccc
     1441 gtccccggcc cgtagaacag accgccccgc aggatgaccc agtcgagccc ggaggctcgc
     1501 acggcctcct ccatgtcgag cgtcgcggcg atggcgcggg ccgagacgtc gtcgctgtcc
     1561 ggccgccgga cggtgtcctc gtcgaccagc gcctccccgg ccgccccgac catggcgatg
     1621 ctctgttgca gcagacgggt gacgcccgtc tggcggcagg cctccagcaa cagcggaacg
     1681 ccctcgcggc gcaccctgtc gttgacgtca tagtcgccgc ggccgctggg gccgggcagg
     1741 gatgtcgcca ggttgatgca ggtgtcgcag ccgtccaggc cgcggcggag gctgtccggg
     1801 tcaaagatgt ccgccgcccg gacctcggcc ccgcatgccc gcgccacgcc cgcggcctcg
     1861 actcgccgca ccagggcgcg gacgcgatag cccgccgcga ccagtcgggg ggtcaggtgt
     1921 cgcccgtaga cgccattggc gccgaggatg gccacggtgg gtttcatcgg ctgcaacctt
     1981 cgttgacgga ccagcctagc aagtgttcgt attttgttcc agaggcgagt tcggcccgcc
     2041 gccggccccc tccgggacga ccgccggcct agctccggta gtcgccgttg atcgccacgt
     2101 actgcttggt caggtcgcag gtccagaccg tcgccacgcc ccggccgacg ccgacgtcga
     2161 tgctgacctc gagctccggc ttcttcatgt aggcgctcat cctggcctcg gagtaggtcg
     2221 gcgagaccat gccgtcgtgg gcggcgacca gcttgccgaa ggcgacgctc atcttctcgc
     2281 ggttgaccgg ctcgtcggcc cggccgaccg ccatgacgat acggccccag ttggcgtcct
     2341 cgccggcgaa ggcggtcttg accagcgggc tttcggccac cgtgcgggcg atcttgcggg
     2401 cgctggcggc gctctcggcc cccgtgacgt tgatcttgac gaacttggtc gccccctcgc
     2461 catcgcggac cagctggagg gccaggttca gcagcagggc ctcgagcttc tcgcggaagt
     2521 cggccagccg gtggtcgccg gcccggctga tgcgcggcgc gcccgaggcg ccggtggcga
     2581 acagcagcag ggtgtcgttg gtcgagcggt cgccgtcgac cgtgacggcg ttgaacgtcg
     2641 tgcgcgtata gaggctgacc agggtctgca gcgccttggg ggcgatggcc gcgtccgtgg
     2701 cgacaaaggc cagcatggtg gccatgtcgg gcgcgatcat gcccgagccc ttggcgatgc
     2761 cgttgatgcg gacggtcctg ccgtcgatct tcgcggtggc cgtggcgccc ttggcgaagg
     2821 tatcggtggt catgatggcg cgggccgcgt cggcccagcc gtcgtcggtc aggcggccat
     2881 cgacctcggg caggcggctg gtgatcttgc cgtcgtccag caccacgccg atgaccccgg
     2941 tggacgccat catcacatcg cgctggcggc agtcgaagcg cttggccacg gccccggcga
     3001 cccgcctcac ggcgtccgcg ccgggcttgc ccgtgaagct gttggcgcag ccggcattga
     3061 ccaccagggc ctgaacaccc tcgccgcccg agttttcgag ctgcttcttg caccagtcga
     3121 ccggcgccga cccgacaccg tggcgcgtga acaccccgcc gcagctggtg cccttggcga
     3181 agcgcatgac cagcagatcc ggccgctcgt gcttgtagaa gccggcccgg ccgataccga
     3241 tctggacacc gccgatcggc ggcatcttcg ggaagggcac ggccagcggc gagacggcca
     3301 ggccaggctt gcccggcgcg ccggccggtt cggccgcggc ggcattggcc gggcgtttgg
     3361 tggtggccc
//
LOCUS       DAORNX010000341         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21269_length_3365_cov_5.791239, whole genome shotgun sequence.
ACCESSION   DAORNX010000341 DAORNX010000000
VERSION     DAORNX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21269_length_3365_cov_5.791239"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PLO65_13205"
     CDS             complement(<1..155)
                     /locus_tag="PLO65_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRD29250.1"
                     /translation="MGQGGHGPQDHGLQGLMPSAALDRTLAALADPHRRRVVELLRER
                     PRRAGDLA"
     gene            complement(107..574)
                     /locus_tag="PLO65_13210"
     CDS             complement(107..574)
                     /locus_tag="PLO65_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD29251.1"
                     /translation="MPAKPALSSALVYRDPMAALKWLEKAFGFETTLLIEDADGNLAH
                     SQMAWGDSLVMVGNEWTDEHRSPASIGGLMTQTVHIQLADGQGTVDEHCARARKAGAE
                     IIVEPETQFYGDRTYRCKDPEGHIWTVSQTVQVMAPDEWDKAGMGLKTTVYKA"
     gene            complement(655..1878)
                     /locus_tag="PLO65_13215"
     CDS             complement(655..1878)
                     /locus_tag="PLO65_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HRD29252.1"
                     /translation="MSRLIRRILLAVSAALLLAGPAAAAERAPLILISIDGFRADYAT
                     RGLTPTLAALAADGAWAREGMRPSFPSNTFPNHYTLVTGLRPDHHGITDNTLYDAARP
                     DVKFSMGAREQVQDAFWWNDGEPVWVTAERAGLKTATMYWPGSEAAVQGVRPTTWSIY
                     DEAVTAFDRVDRLLGWLDRPAAERPAFLTLYFEAVDTVGHRGGPDGPDMPKALGEVDA
                     ALARLVAGLKARGLYDKVNIVAVGDHGMMEITPERVIFLDDVTAPESFRWITLGGFAG
                     LVPLTPAAEAALLAEHGHMECWRKGEIPARYHYGSHRRIPPVICLAQTGWIITTRAWY
                     ARRPMTGPAGSHGFDPEDVRMRALFVAHGPDIRPGVLLPVFDNVSVYPLLMALLGLTP
                     RPNDGDPAATAPALR"
     gene            2000..2893
                     /locus_tag="PLO65_13220"
     CDS             2000..2893
                     /locus_tag="PLO65_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD29253.1"
                     /translation="MNFALEAAGVSKTYGTVKALDGLSLNIPRGGIFGILGPNGAGKS
                     TLFRIVLGLVRPTIGETRLLGEKAGDIKALRRIGAVIETPRFLPWLTGRDTLRMLALE
                     SGEKAADLDGWLARVGLTEAADRKVSGYSVGMKQRLALAAALLTRPELLILDEPTSGM
                     DPAGILEMRALMRELADRDGVTVILASHQLDEVQRVCDAVAILDRGKLVAEGRVDALT
                     GINERLRLLASPTAKVLEILGDRGVADGPDAVFAALPRAEAPALIAALVKAGIEITEA
                     RWVGGDLEKVFLSHTGETDAG"
     gene            2883..>3365
                     /locus_tag="PLO65_13225"
     CDS             2883..>3365
                     /locus_tag="PLO65_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD29254.1"
                     /translation="MLADAFAAERLRFFKARGTVFWSLAFVPLFGLALGVIQGLFMRN
                     LLHAAGARGEAAAALGRAPVYLLSQAVESVSSSNFFIVQLFFLMAGSAIFAGDYRWET
                     WRFLTPRNTRANLLLGKLATFGLAALIGLGLLAIGGVLTGVASAALTGGDIGWARPGD
                     D"
BASE COUNT          478 a         1156 c         1221 g          510 t
ORIGIN      
        1 gctagatccc cggcgcggcg cggccgctcg cgcagcagct cgaccacccg ccggcggtgc
       61 ggatcggcca gggcggccag ggtgcggtcg agggcggccg agggcatcag gccttgtaga
      121 ccgtggtctt gaggcccatg cccgccttgt cccactcgtc gggggccatg acctggaccg
      181 tctggctgac cgtccagatg tgcccctcgg ggtctttgca gcgataggtg cgatcgccat
      241 agaactgggt ctccggctcg acgatgatct ccgctccggc cttgcgggcc cgcgcgcagt
      301 gctcgtccac cgtcccctgg ccgtcggcca gctggatatg cacggtctgg gtcatcaggc
      361 cgccgatcga ggccgggctg cggtgctcgt cggtccattc gttgccgacc atcaccaggc
      421 tgtcgcccca ggccatctgc gaatgggcca ggttcccgtc cgcgtcctcg atcagcaggg
      481 tggtctcgaa gccgaaggcc ttctcgagcc atttcaacgc cgccatcggg tcgcgataga
      541 ccagggcgga cgacagggcg ggtttcgcgg gcacggcggc ctcccgtttc aattatcgca
      601 atatttcacg catatagaaa ctatctcgtc aacctggcgc ttccgcccac ggactcagcg
      661 cagggcgggc gcggtggccg ccgggtcgcc gtcattcggg cgcggcgtca agcccagcag
      721 ggccatcagc agcggataga ccgagacgtt gtcgaagacc ggcagcagca cgccgggccg
      781 gatgtcgggg ccgtgggcga cgaacagcgc ccgcatgcgc acgtcctccg gatcgaagcc
      841 gtgcgaaccg gccggaccgg tcatcggccg gcgggcgtac caggcgcggg tggtgatgat
      901 ccagccggtc tgggccaggc agatgaccgg cgggatgcgc ctgtggctcc cgtagtgata
      961 gcgggccgga atctcgccct tgcgccagca ctccatgtgg ccgtgctcgg ccagcagggc
     1021 cgcttccgcc gccggcgtca acggaaccag tccggcgaac ccgccaaggg tgatccagcg
     1081 gaagctctcg ggcgcagtga cgtcatcgag gaagatgacc cgctcgggcg tgatctccat
     1141 catcccgtga tcgccgaccg cgacgatgtt gaccttgtca tagaggccgc gcgccttcag
     1201 cccggcgacc agccgggcca gggccgcgtc gacctcgccg agggccttcg gcatgtccgg
     1261 gccatccggt ccgccgcgat ggccgacggt gtcgaccgcc tcgaaataca gcgtcaggaa
     1321 ggcggggcgt tcggccgcgg gccggtccag ccagccgagc agccggtcga cgcggtcgaa
     1381 ggccgtgacc gcctcgtcgt agatcgacca ggtggtcggg cgcaccccct gcaccgccgc
     1441 ttcggaaccg ggccagtaca tcgtcgccgt cttcagaccg gcgcgctcgg ccgtcaccca
     1501 gaccggctca ccgtcgttcc accagaaggc gtcctgcacc tgttcgcggg cgcccatgct
     1561 gaacttcacg tccgggcggg cagcgtcgta gagggtgttg tcggtgatcc cgtggtgatc
     1621 ggggcgcagg ccggtgacca gggtgtagtg gttggggaag gtgttgctcg ggaacgacgg
     1681 ccgcatgccc tccctggccc aggccccgtc ggcggcgagg gccgccaggg tcggcgtcag
     1741 gccacgggtg gcgtagtcgg cgcggaagcc gtcgatggag atcagaatca acggcgcccg
     1801 ctccgcggcg gcggccgggc cggccagcag cagcgccgcc gaaacggcca gaagaatgcg
     1861 gcggatgagg cggctcatgg tcttcccccc gggagtcagg cgcccatctg gaccggcgcg
     1921 cttggcggac tcaagcgacc tcgtcatagt cggcttctgt cacgccgcaa tgaccgggag
     1981 cggaccgccg gatgacccga tgaatttcgc cctcgaggcc gccggggtca gcaagaccta
     2041 cgggacggtc aaggcgctgg acgggctgtc gctgaacatc ccgcgcggcg gcatcttcgg
     2101 catcctcggc cccaacggcg cgggcaagag caccctgttc cgcatcgtgc tgggcctggt
     2161 ccggccgacc atcggcgaga cccggctgct cggcgagaag gccggcgaca tcaaggccct
     2221 gcgccggatc ggtgcggtca tcgagacgcc gcgcttcctg ccctggctga ccgggcgcga
     2281 cacgctgagg atgctggccc tcgagtcggg tgagaaggcg gcggacctcg acggctggct
     2341 ggcccgggtc gggctgaccg aggcggcgga ccgcaaggtg agcggctatt ccgtcgggat
     2401 gaagcaacgg ctggccctgg ccgccgccct gctgacacgg cccgagctgc tgatcctcga
     2461 cgagccgacc agcggcatgg atccggccgg gatcctcgag atgcgcgccc tgatgcgtga
     2521 gctggccgac cgtgacgggg tgaccgtcat cctggccagc caccagctgg acgaggtgca
     2581 gcgggtctgc gacgcggtgg ccatcctcga ccgcggcaag ctcgtggccg agggacgggt
     2641 cgacgccctg accggcatca atgagcggct gcggctgctg gcgagcccga cggccaaggt
     2701 gctggagatt ctcggcgacc gcggcgtggc cgacggcccg gacgcggtgt tcgcagccct
     2761 gccccgcgcc gaggcgccgg cgctgatcgc cgcgctggtc aaggccggta tcgagatcac
     2821 cgaggcccgc tgggtcggcg gcgacctgga gaaggtgttc ctcagccata cgggagagac
     2881 cgatgctggc tgacgccttc gccgccgagc gactgcgctt cttcaaggcg cgcgggacgg
     2941 tgttctggag cctggccttc gtgccgctgt tcgggctggc gctgggcgtg atccagggcc
     3001 tgttcatgcg caacctgctg cacgcggccg gcgcgcgcgg cgaggcggcg gccgccctgg
     3061 gacgggcgcc ggtatacctg ctgagccagg ccgtggaatc cgtttccagc tccaacttct
     3121 tcatcgtgca gctgtttttc ctgatggccg gctcggcgat attcgccggc gactaccggt
     3181 gggagacctg gcgcttcctg acgccgcgca acacccgggc caacctgctg ctgggcaagc
     3241 tggccacttt cggcctggcc gcgctgatcg gactggggct gctggcgatc ggcggcgtgc
     3301 tgaccggcgt ggcctccgcc gccctgaccg gcggcgacat cggctgggcg cggcccggcg
     3361 acgac
//
LOCUS       DAORNX010000342         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21324_length_3359_cov_6.463378, whole genome shotgun sequence.
ACCESSION   DAORNX010000342 DAORNX010000000
VERSION     DAORNX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21324_length_3359_cov_6.463378"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLO65_13230"
     CDS             <1..409
                     /locus_tag="PLO65_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29255.1"
                     /translation="SAGPDAAAPLGLTPVSAPPPWSPIVVRGLTLAALAALGEAGEAN
                     LDSLTSLSAEPNAAYCLNMAKLNLYQCLAVSKPHYEDIFCLGQHILIDTGSCMVKWTG
                     ATMPPEPVRPPVVTASGPKKKTTASAAAPTRRR"
     gene            531..1142
                     /locus_tag="PLO65_13235"
     CDS             531..1142
                     /locus_tag="PLO65_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRD29256.1"
                     /translation="MAEIVLNVEVRDRTGTGGAREARRAGKVPGVLYGGPKGPVAISV
                     KANEFRKSLYTGQLHGHLVTLKYGDESQPVIAKDIQFHPVTDEPVHFDLLRVEEGSTI
                     RIAIPVHFKNQEVCPGLKQGGSLEIVRHDVDLNCPADAIPEELVFDLSNSQLGDTIRI
                     GDITLPKGVTPVVADRDFVLATLKVSSAGMSEAAEAAEGEAEA"
     gene            1236..1856
                     /gene="pth"
                     /locus_tag="PLO65_13240"
     CDS             1236..1856
                     /gene="pth"
                     /locus_tag="PLO65_13240"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRD29257.1"
                     /translation="MILAGLGNPGPKYEKNRHNVGFMAVDAIARRWATAPWRARFQGL
                     ACEGEVTTPDGPIRLLMLKPQTYYNETGRAVGEAARFFKLKPAEVIVFHDEIDLAPGR
                     FRMKTGGGAAGNNGIRSVTSQVGADFRRARIGVGHPGDRDAVMHYVLGDFPKADRVWL
                     DPMLDALADALPFAAAGDDDRYQAEVLRLAPAAKADPRKQARDDQP"
     gene            2120..2569
                     /locus_tag="PLO65_13245"
     CDS             2120..2569
                     /locus_tag="PLO65_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRD29258.1"
                     /translation="MTTVFVDSNVVIYSLDRRSPEKRESARAWLKHLGPSDLIVMSPQ
                     VLNEAYAVLTLKHRLDPVADGVRAMLVRFERWVIAPLDFSTVLAAWALQDRYDVRIWD
                     ALLLASANAAGCAHFLTEDLNDGQVYGVVQAINPFRHMPEDVLGRAL"
     gene            2597..>2813
                     /locus_tag="PLO65_13250"
     CDS             2597..>2813
                     /locus_tag="PLO65_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD29259.1"
                     /translation="MALKVAIVGLPNVGKSTLFNALTQTASAQAANYPFCTIEPNTGD
                     VAVPEPRLNALAEIAGSKEIIPARITFV"
     assembly_gap    2814..2964
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2965..>3359
                     /locus_tag="PLO65_13255"
     CDS             <2965..>3359
                     /locus_tag="PLO65_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRD29260.1"
                     /translation="DLEIIETELMLADMESLERRRPQLEKRAKGGDKESALTLKLVDL
                     ALAQLNAGKPARTAEVSKEDKKAWDMLQLLTALPALYVANVEEGSADKGNDLSAKVAA
                     RAAQDNANSVVISAQIESEIAVLDDEEQK"
BASE COUNT          554 a         1149 c         1037 g          468 t
ORIGIN      
        1 gagcgccggc cccgacgccg ccgcgccgct cggcctgact ccggtctcgg ccccgccgcc
       61 ctggtcgccg atcgtcgtgc gtggcctgac cctggccgca ctggccgcgc tgggcgaggc
      121 cggcgaagcc aatctcgact cgctcaccag cctgtcggcc gagccgaacg ccgcctactg
      181 cctgaacatg gccaagctga acctctacca gtgcctggcc gtgtccaagc cgcactacga
      241 ggacattttc tgcctcggcc agcacatcct gatcgatacc ggctcctgca tggtcaagtg
      301 gacgggggcg accatgccgc ccgagccggt ccggccgccg gtcgtcacgg ccagcgggcc
      361 gaagaagaaa accaccgcct cggcggcggc cccgacgcgc cgtcgataga ctcggcggcg
      421 gcgttgcgcc gcccgctgaa agcgtgtatc gagcgcaacc ctgaattcac cgaccgtcgc
      481 cgcgcggagc ctttctgccg cggcgacttc gttttgagaa ccgtgagacc atggccgaga
      541 tcgtcctgaa cgtcgaagtc cgcgaccgca ccggcacggg cggcgcccgt gaagcccgcc
      601 gcgccggcaa ggtgccgggc gtgctctacg gcggccccaa gggtcccgtg gccatctcgg
      661 tcaaggccaa cgaattccgc aagtcgctct acaccgggca gctgcacggc catctggtga
      721 cgctgaagta cggcgacgag agccagccgg tcatcgccaa ggacatccag ttccatcccg
      781 tcacggacga gccggtgcac ttcgacctgc tgcgcgtcga ggaaggctcc accatccgca
      841 tcgccatccc ggtgcacttc aagaaccagg aggtctgccc gggcctcaag cagggcgggt
      901 cgctggaaat cgtgcgccac gacgtcgacc tcaactgccc ggccgacgcc atcccggaag
      961 agctggtgtt cgacctgagc aacagccagc tcggcgacac catccgcatc ggcgacatca
     1021 ccctgcccaa gggcgtgacc ccggtcgtgg ccgaccgcga cttcgtgctg gccaccctga
     1081 aggtctcctc ggccggcatg tcggaagccg ctgaagcggc cgaaggcgaa gctgaagcct
     1141 gatacgggcc tcgcattgtt gctggaaacg ggcgggccta ggctcgcccg ttttcgtttg
     1201 agcccatcgg acggcgcagg gagaccgacg tgacgctgat cctcgccggc ctcggcaacc
     1261 cggggcccaa gtacgaaaag aaccgccaca acgtcggctt catggccgtc gacgccatcg
     1321 cccgccgctg ggccacggcc ccctggcgcg cgcgcttcca gggtctggcc tgcgagggcg
     1381 aggtcacgac gccggacggg ccgatcaggc tgctgatgct gaagccccag acctattaca
     1441 acgagaccgg acgcgcggtc ggcgaggcgg cgcgcttctt caagctcaag cccgccgagg
     1501 tgatcgtctt ccatgacgag atcgacctgg cccccggccg cttccgcatg aagacgggcg
     1561 gcggggcggc gggcaacaac ggcatccgct cggtgaccag ccaggtcggc gccgacttcc
     1621 gccgcgcccg catcggcgtc ggccatccgg gcgaccgcga cgcggtaatg cactacgtgc
     1681 tcggcgactt tcccaaggcc gaccgggtct ggttggaccc gatgctcgac gccctggccg
     1741 acgccctgcc gttcgcggcg gccggcgacg acgaccgcta ccaggccgag gtcctgcggc
     1801 tggccccggc ggccaaggcc gatccccgca aacaggcgcg cgacgaccag ccttgacacg
     1861 aagtccgtgg atcacgaagt tcgtgtgatg aagaacgtca ccgtcagcat ggatgaggac
     1921 acactgacct gggtgcgggt cgaggcggcc cgggctggcc agagtgtctc gcgctggctg
     1981 ggtgagcgcc tctcgcgcga acgggccggc gccgacgaag ccacagcgag gcgggcggcc
     2041 ggacggcagg cgatccaggc catcctcgat catcccggct gggaccttgg cgctgcacga
     2101 ctcgacaggg acgaaatcta tgacgaccgt cttcgtggac tcgaacgtcg tcatctacag
     2161 cctggaccgg cggagcccgg agaagcgcga gagcgcgcgg gcgtggctga aacacctcgg
     2221 cccttccgat ctgatcgtga tgagtcctca ggttctgaat gaggcctacg cggtcctgac
     2281 cctcaagcat cgcctcgacc ccgtcgccga tggtgtccgc gccatgctgg tccggttcga
     2341 gcgctgggtg atcgcgccgc tggacttcag cacggtcctc gcggcctggg cgctgcagga
     2401 tcgttacgac gtcagaatct gggacgccct cctgcttgct tccgccaacg cggccggctg
     2461 cgcccacttc ctcaccgagg atctcaacga tggccaggtc tacggcgtcg ttcaggcgat
     2521 caatcccttt cgccatatgc ccgaggacgt gctaggtcgc gccctctgaa cgccctgccc
     2581 aatcccgaaa gctccaatgg ccctgaaagt cgcgatcgtc ggcctgccca acgtgggcaa
     2641 gtcgaccctg ttcaacgccc tgacccagac cgcgtcggcc caggccgcca actatccgtt
     2701 ctgcaccatc gagccgaaca ccggcgacgt ggccgtgccg gagccgcgcc tgaacgcttt
     2761 ggccgagatc gctggctcca aggagatcat ccccgcccgc atcaccttcg tcgnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnncgacct ggagatcatc gagaccgagc tgatgctggc
     3001 cgacatggaa agcctggagc gtcgtcggcc ccagctggag aaacgggcca agggcggcga
     3061 caaggaatcg gccctgacgc tgaagctggt cgatctggcc ctggcccagt tgaacgccgg
     3121 caagcccgcc cgcaccgccg aagtgtccaa ggaggacaag aaggcctggg acatgctgca
     3181 gctgctgacc gccctgcccg ccctctatgt cgccaatgtc gaggaaggct cggccgacaa
     3241 gggcaacgac ctgtcggcca aggtcgcggc ccgcgcggcc caggacaacg ccaactccgt
     3301 cgtcatctcg gcccagatcg aatccgagat cgccgtgctg gacgacgagg agcagaaag
//
LOCUS       DAORNX010000343         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21348_length_3356_cov_6.362920, whole genome shotgun sequence.
ACCESSION   DAORNX010000343 DAORNX010000000
VERSION     DAORNX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21348_length_3356_cov_6.362920"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLO65_13260"
     CDS             <1..215
                     /locus_tag="PLO65_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRD29261.1"
                     /translation="QPDAAAIRGMVEGLAARLEKTPDDPQGWVRLVRAWSVLGETAKR
                     DAALARARALYKGRPDILKALDEAAQ"
     gene            212..691
                     /gene="ccmE"
                     /locus_tag="PLO65_13265"
     CDS             212..691
                     /gene="ccmE"
                     /locus_tag="PLO65_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRD29262.1"
                     /translation="MSFWPKSRTARRRLTILLAIAPVLALAAGLTLWGLRDSISLFYT
                     PAQAREAHVAPGRAVQLGGLVLPGSVVKHPDGQVDFAISDQTETVRVTYRGDLPDLFR
                     EGQGVVAEGTFSEDGVFRAKRVLAKHDEKYMPRDVAKALKKQGEWRGDGASPTYAKP"
     gene            688..2646
                     /locus_tag="PLO65_13270"
     CDS             688..2646
                     /locus_tag="PLO65_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRD29263.1"
                     /translation="MTVELGAFALTLALALSFAQTGLGFAGARRPVLAGAGEGAAIGA
                     FLAVAVAFVVLVAAFVASDFSVANVAANSHTAKPLLYKVAGAWGSHEGSMLLWCLILT
                     GYGAAVAGFGRGLPAALKSRVLGVQGLLGLLFLAYTVFASNPLARLLTAPIEGRSLNP
                     LLQDPALAAHPPFLYLGYVGFSVVFSFAVAALIGGRVDAAWARWVRPWTLAAWSFLTI
                     GIALGSFWAYYELGWGGWWFWDPVENASFMPWLIGAALLHSAVVTEKRGALSGWTVFL
                     ALAAFTFSMLGAFLVRSGVLTSVHAFAVDPTRGVLLLSILGVASGAAFALFAWRAPAL
                     PPGGVFAPASRESLIVLNNILLTAATATVMLGTLFPLIREAIGGETVSVGPPYFNLTF
                     APLMALAMLVLPFGPLTAWKRGDLVAGAERLKLAAAAAVLSGLAVFGLVSPRKALASA
                     GVALGAWLIAGAVAEVAERAKAFRAPWPEVRRRLTGLPRGAWGMTLAHAGLGVFILGA
                     CFETAWKVEAAEALSVGGHLRLGAFELTLKDVGTIDGPNYLAERGQVAVTRAGRPVCA
                     PTPERRFYPAGRQTTSEVALCNLGFSQVYVVLGERRAGADGAPAWLVRSYYNPWVQLI
                     YLGPLLMALGGLLSLSDRRLRFAVGRRP"
     gene            2643..3092
                     /locus_tag="PLO65_13275"
     CDS             2643..3092
                     /locus_tag="PLO65_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRD29264.1"
                     /translation="MRNRLLLALAAALSLAAASDPSERLPDPAQEARARTLFSQVRCL
                     VCQNESIDDSDAELAADLRRVVREQVAAGRSDAQVKRFLTDRYGEFVLLKPAFSPGNA
                     ALWLAPFLAVVAGLVLLAARWRRREGTADLSGEEETRLSALLDDEGP"
BASE COUNT          401 a         1200 c         1204 g          551 t
ORIGIN      
        1 cccagcccga cgccgcggcc atccgcggca tggtcgaagg cctggcggct cgcctcgaga
       61 agactcccga cgacccccag ggctgggtgc ggctggtccg cgcgtggtcg gtgctggggg
      121 aaaccgccaa gcgggacgcc gccctggctc gcgcccgcgc cctctacaag gggcgtcccg
      181 acatcctcaa agcgctcgac gaggccgccc aatgagcttc tggccgaaat cccgcaccgc
      241 gcggcgccgg ctgacgatcc tgctggccat cgcgcccgtg ctggcgctgg cggcggggct
      301 caccctgtgg ggcctgcggg attcgatctc gctgttctac acgccggcgc aggcccggga
      361 ggctcatgtc gcgcccggcc gcgccgtgca gctcggcgga ctggtgttgc cgggcagcgt
      421 ggtcaagcat cccgacgggc aggtcgattt cgcgatctcc gaccagaccg agaccgtccg
      481 cgtaacctat cgcggagatc tgcccgacct gtttcgcgag ggtcagggag tcgtcgccga
      541 ggggaccttc agcgaggacg gcgtgttccg ggccaaacgg gttctggcca aacacgacga
      601 gaaatacatg ccgcgcgacg tcgccaaggc cctgaagaag cagggcgagt ggcgcggcga
      661 tggcgccagc ccgacctacg ccaagccatg accgtcgaac tcggcgcctt cgccctgact
      721 ctcgccctgg cgctgtcctt cgcgcagacg gggctcggct tcgccggcgc ccggcggccc
      781 gtactggccg gagccgggga gggggccgcc atcggagcct tcctggccgt cgccgtagcc
      841 ttcgtcgtgc tcgttgcggc tttcgtcgcg tcggacttct ccgtggccaa cgtggccgcc
      901 aactcccaca cggccaagcc tttgctgtac aaggtggccg gcgcctgggg cagtcacgag
      961 ggctccatgc tgctctggtg cctgatcctg acgggctacg gcgccgccgt cgccggcttc
     1021 ggccggggcc tgcccgccgc tctgaagagc cgggttctgg gcgtgcaggg cctgctgggc
     1081 ctgctgttcc tcgcctacac cgtgttcgca tccaatccgc tggcccggct gctgaccgcg
     1141 ccgatcgagg ggcgctcgct caacccgctg ctgcaggacc cggcgctggc cgcccatcca
     1201 ccgttcctct acctcggcta cgtcggcttc tccgtcgtct tctcgttcgc cgtcgcggcc
     1261 ctcatcgggg ggcgcgtgga tgcggcctgg gcccgctggg tgcggccctg gacgctggcc
     1321 gcctggagct tcctgaccat cggcatcgcg ctgggctcgt tctgggccta ctacgagctc
     1381 ggctggggcg gctggtggtt ctgggatccg gtcgaaaacg cctcgttcat gccctggctg
     1441 atcggcgccg cgctgctgca ttcggcggtg gtcaccgaga agcgcggggc cctgtcaggc
     1501 tggacggtct tcctggcgct ggcggccttc accttctcca tgctcggcgc ctttctcgtg
     1561 cggtccggcg tgctgaccag cgtgcacgcc ttcgccgtcg atccgacccg cggcgttctg
     1621 ctgctgtcga tcctcggcgt cgcctcgggc gcggccttcg ccctgttcgc ctggcgcgcg
     1681 cccgccctgc cgccgggcgg cgtgttcgct ccggcgagcc gcgaaagcct gatcgtgctc
     1741 aacaacatcc tgctgaccgc ggccaccgcc acggtgatgc tgggcacgct gtttccgctg
     1801 atccgcgagg ccatcggcgg cgagaccgtc tccgtcgggc cgccctactt caacctgacc
     1861 ttcgcgccgc tgatggcgct ggcgatgctg gtgctgccgt tcgggcccct gaccgcctgg
     1921 aagcggggcg atcttgtcgc cggggcggag cgcctgaagc tggccgccgc cgccgccgtg
     1981 ctgtcgggcc tggccgtgtt cggcctggtc agtccgcgca aggcgctggc aagcgccggg
     2041 gtcgccctcg gcgcctggct gatcgccggg gccgtggcgg aggtcgccga gcgggccaag
     2101 gcgttccgcg cgccctggcc cgaagttcgc cgccgcctga ccggccttcc gcggggcgcc
     2161 tggggcatga ccctcgcaca cgccgggctg ggcgtcttca ttctgggcgc ctgtttcgag
     2221 acggcgtgga aggtcgaggc cgccgaggcg ctgtcggtcg gcggtcacct gcggctgggc
     2281 gcgttcgagc tgacgctcaa ggatgtcgga accatcgatg gccccaacta tctcgccgaa
     2341 cgcggccagg tcgccgtgac ccgggccgga cggccggtct gcgcgccgac gccggagcgg
     2401 cgtttctacc cggccgggcg gcagacgacc tccgaagtgg ccctgtgcaa cctcggcttc
     2461 agccaggtct atgtcgtgct gggcgagcgc cgcgcggggg cggacggcgc gccggcctgg
     2521 ctggtgcgca gctactacaa tccctgggtc cagctgatct atctgggacc gctgctgatg
     2581 gccctgggcg gcctgctgtc gctgtcggac cggcgactgc gcttcgccgt gggacgcagg
     2641 ccatgaggaa ccggctgctt ctggcgctgg ccgcggcgtt gagcctcgcg gccgcctccg
     2701 acccgtcgga gcgcttgccc gatccggccc aggaggcccg ggcgcggacg ctgttcagcc
     2761 aggtgcgctg cctcgtgtgc cagaacgaat cgatcgacga ttccgacgcg gagctggccg
     2821 ccgatctgcg acgggttgtg cgcgagcagg tcgccgcggg gcgttccgac gcccaggtaa
     2881 agcgtttcct gaccgaccgc tatggcgagt tcgtgctgct caagccggcc ttcagcccgg
     2941 gcaacgccgc gctctggctg gccccgttcc tggctgtcgt cgcgggcctg gtcctgctgg
     3001 ccgcacgatg gcgccgccgc gaggggaccg ccgatctcag cggcgaggaa gaaacgcgac
     3061 tgtcggcctt gctggacgac gaaggcccgt gacacgattg cgccgcgatc cggccccaag
     3121 aagcccatgg tcggatgacg gaaagataac ttgagagcgg tcacgctctt tggcatcgaa
     3181 aacagcaaac gggcccgcgc aggcgggaca ggaacggaac acagatggtc gccaggaaga
     3241 ccggattcgt tgtgggcgcc gtcgccggcg tgggcgtcgc cgccgccgcg ctggccggcg
     3301 tgggattgcg catgtcgccg gcgcaggccg ccgagccggc ccagctggtg cggacg
//
LOCUS       DAORNX010000344         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21510_length_3339_cov_8.386419, whole genome shotgun sequence.
ACCESSION   DAORNX010000344 DAORNX010000000
VERSION     DAORNX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21510_length_3339_cov_8.386419"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1208
                     /locus_tag="PLO65_13280"
     CDS             192..1208
                     /locus_tag="PLO65_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD29265.1"
                     /translation="MQSKRLGRSAIAVSTICMGTMTFGSQADEAMAHRILDRSLDAGI
                     NFFDTAENYPVPPREDWAGVTEEIVGRWMKTRRRDELIIATKVCGPSHGWLKASQRAG
                     MTALDRHNIVRAVEASLKRLGTDYIDLYQTHWPDHGARYEEALRALDDLIEAGKVRMI
                     GCSNETAWGLMKSLAVSEREGLARYETIQNNFSLNNRRFEDALSQVCLQEGVSLIPYS
                     PLGGGVLSGKYNGGARPAGARFSNYLELGGRQAAMAQRFVNDKSLSATERFMAIAAEA
                     GLAPVTLATAWSKQHDFVASTIVGATHEDQLDDIFAAADLVLDAEVMKKIDAVSRDIL
                     YPMG"
     gene            1311..2771
                     /locus_tag="PLO65_13285"
     CDS             1311..2771
                     /locus_tag="PLO65_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC5 family protein"
                     /protein_id="HRD29266.1"
                     /translation="MQTSFVQWAVFGGLTLLMAVLTGWQVRRMGGRSRDSNREYFLAG
                     GTLTWPFIAGSITLTNLSTDQLVGMNGNQMALLAWWELSAIVGLLMLAYIFLPIYYRN
                     HCTTTTELLERKYGSIHLRAVVSVLFLLGNVFLYLPIMLYTGSLFLKTMFGIEIPLIL
                     IAAAMAVLGGAYAISGGLRAVAVYDTVSGVGVLAMALLVVVLALAAIDFDLSGIPADR
                     LSMIGGPDSPIPWPTLLTGMIFIQMFYWSTNQTITQRAMAAPTLREGQKGVLAAALIR
                     LLIVPAIVVVPGIAAFKLFGDAGDATYGRVVAMVMPGWLSGAFAAFMAAAVLTSYTSV
                     LNSSVTLYVCDLHEKFIAEKPDVGRLNRIISVAFMVLSVALVPLYMGAASIINLVQQL
                     NGLLSMPILSAFIVGLMFRDVDARSVMGAVVFGTLLYAVFTFLWTPVHYIHLMLVTLL
                     ACVAFALSINRLLFGRRAALAPSVAGALRRVVGART"
     gene            2816..3091
                     /locus_tag="PLO65_13290"
     CDS             2816..3091
                     /locus_tag="PLO65_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29267.1"
                     /translation="MNEPRAARPGDRRARTRDQLLVAAQTLLLDREAGPLGIRRITTA
                     AGLVHGSFYNYYPDLDALLDDLAALMFASHAALVSGPVSTIRPSLSP"
     gene            3115..>3339
                     /locus_tag="PLO65_13295"
     CDS             3115..>3339
                     /locus_tag="PLO65_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29268.1"
                     /translation="MTDEPTYGRLLFDSGLPVDRFVTGLRGAMGADIVEGVRCGRFAA
                     PDVAIAISLTAGAILGAGIDLHRGRLGAAAI"
BASE COUNT          496 a         1173 c         1109 g          561 t
ORIGIN      
        1 cggggccatc cagcccggcg cgaccgtgcg catcgcgccg cgggccgtgc gtctgttcgc
       61 cgccgccggg cccgccgatc gcatctgaag gccacaggtt gggcttggct tcccgcgggg
      121 gagcggccta ggaggagcgt gaccgggccg cggcgacgcg cgccccccac gcctcaagcc
      181 ggagacttcc catgcaaagc aaacgactgg gccgaagcgc catcgcggtg tccacgatct
      241 gcatgggcac gatgaccttc gggtctcagg ccgacgaagc gatggcccac cggatcctcg
      301 accggtcgct ggacgcaggg atcaacttct tcgacacggc cgagaactat ccggtgccgc
      361 cgcgcgagga ctgggccgga gtcaccgagg aaatcgtcgg ccgctggatg aagacccgcc
      421 gccgcgacga actgatcatc gccaccaagg tctgcggtcc cagccacggc tggctgaagg
      481 cctcgcagcg ggccggcatg accgccctgg accggcacaa catcgtgcgg gccgtcgagg
      541 ccagcctcaa gcggctcggc accgactaca tcgacctgta ccagacccat tggcccgacc
      601 acggcgcccg ctacgaggaa gccctgcggg ccctcgacga cctgatcgag gccggcaagg
      661 tgcggatgat cggctgcagc aacgagacgg cctggggcct gatgaagagc ctggcggtct
      721 ccgaacgcga gggcctggcc cgctacgaga cgatccagaa caatttcagc ctcaacaacc
      781 gccgcttcga ggacgccctg tcgcaggtct gcctgcagga gggtgtcagc ctgattccct
      841 attcgccgct gggcggaggg gtcctgtcgg gcaagtacaa cggcggcgca cggcccgccg
      901 gggcccgttt ctccaactac ctggagctgg gtggccggca ggcggccatg gcccagcggt
      961 tcgtcaacga caagagcctg agcgcgaccg aacggttcat ggccatcgcc gccgaagccg
     1021 gcctggctcc ggtcaccctg gccaccgcct ggagcaagca gcacgacttt gtcgcctcga
     1081 ccatcgtcgg cgcgacgcat gaggaccagc tcgacgacat cttcgccgcc gcggacctgg
     1141 tcctcgacgc cgaggtgatg aagaagatcg acgccgtgtc gcgcgacatt ctgtacccga
     1201 tgggctgacg cgctcagacc cctgccgcgc cgggcgaacg cggattgacg aaccggcccg
     1261 gcccggggcg aagtcaaggc ccgcgagtcg gcggtggggg ggtgaagccg atgcagacca
     1321 gtttcgtgca gtgggccgtg ttcggcggcc tgaccctgct catggccgtc ctgaccggat
     1381 ggcaggtccg gcggatgggc ggacgttcgc gggattcgaa ccgggagtac ttcctcgcgg
     1441 gcgggacgct gacctggccg ttcatcgccg gctcgatcac cctgaccaac ctgtccaccg
     1501 accagctggt gggcatgaac ggcaaccaga tggccctgct ggcctggtgg gagctgtcgg
     1561 ccatcgtcgg gctgctgatg ctggcctaca tcttcctgcc gatctactac cgcaaccact
     1621 gcacaaccac gaccgaactg ctcgaacgga agtacggctc gatccatctg cgggcggtcg
     1681 tgtcggttct gttcctgctc ggcaacgtgt tcctgtacct gccgatcatg ctctacacag
     1741 gctcgctgtt cctcaagacc atgttcggga tcgagattcc gctgatcctg atcgccgcgg
     1801 ccatggcggt gctcggcggg gcctacgcga tcagcggcgg cctgcgggcg gtggccgtct
     1861 atgacaccgt ttcaggcgtc ggcgtgctgg cgatggccct gctggtggtg gtcctggccc
     1921 tggccgcgat cgacttcgac ctgtccggga tcccggccga ccggctcagc atgatcggcg
     1981 gccccgactc cccgatcccc tggccgaccc tgctgaccgg catgatcttc atccagatgt
     2041 tctactggag caccaaccag accatcaccc agcgagcgat ggccgcgccg accctgcgcg
     2101 agggtcagaa gggcgtgctg gccgcagcgc tgatccggct gttgatcgtt cccgccatcg
     2161 tcgtcgtgcc cggcatcgcc gccttcaagc tgttcggcga cgcgggcgac gccacctacg
     2221 gccgggtggt ggcgatggtc atgcccggat ggctgtcggg agccttcgcc gccttcatgg
     2281 cggcggccgt gctgaccagc tacacctcgg tgctcaactc gtcggtgacg ctgtacgtct
     2341 gcgacctgca tgagaagttc atcgccgaaa agccggatgt cggccggctg aacaggatca
     2401 tctcggtcgc cttcatggtc ctgtcggtgg cgctggtgcc gctgtacatg ggcgcggcct
     2461 cgatcatcaa cctggtgcag cagctcaacg gcctgctgtc gatgccgatc ctgtcggcct
     2521 tcatcgtcgg cctgatgttc cgcgacgtcg acgcccggtc ggtgatgggg gcggtggtgt
     2581 tcgggacgct gctgtacgcc gtgttcacct tcctctggac gccggtccac tacatccacc
     2641 tgatgctggt gaccctcctg gcctgcgtgg ctttcgccct gtcgatcaac cgtctgctgt
     2701 tcggccggcg cgccgccctg gcgccgtcgg tcgccggcgc cctgcggaga gtggtcggcg
     2761 cgcggacctg agcgccgcaa ttgacataat gtcatttttg acattatgtc agcccatgaa
     2821 cgagccccgt gccgccaggc ccggcgatcg ccgcgccaga acccgcgacc agttgctggt
     2881 cgccgcccag acgttgctgc tggaccgcga ggccgggccc ctcggcatcc gccggatcac
     2941 caccgcggcc ggcctggttc acggcagctt ctacaactac tatccggacc tcgacgccct
     3001 gctcgacgac ctcgcggcgc tgatgttcgc ctcgcacgcc gcgctggtct cagggccggt
     3061 ctcgacgatc cggccgtcgc tttcgccctg atcacccgcc agaccctgca tctggtgacc
     3121 gacgagccca cctacggccg gctgctgttc gacagcggcc tgccggtcga ccgcttcgtc
     3181 accggcctgc gcggcgccat gggggctgac atcgtcgagg gcgtccggtg cggccggttc
     3241 gcggcgccgg acgtcgcgat cgccatcagc ctgaccgcgg gggccatcct cggcgcgggc
     3301 atcgacctgc atcggggccg attgggggcc gcggcgatc
//
LOCUS       DAORNX010000345         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21546_length_3336_cov_7.627553, whole genome shotgun sequence.
ACCESSION   DAORNX010000345 DAORNX010000000
VERSION     DAORNX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21546_length_3336_cov_7.627553"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLO65_13300"
     CDS             complement(<1..206)
                     /locus_tag="PLO65_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase"
                     /protein_id="HRD29269.1"
                     /translation="MDAFPAFFPLTGRKVVIAGAGEAAEAKARLFEGSPAVVVRLEGP
                     NAFLAGAYTGAILAFIVGDDLFVQA"
     gene            complement(217..534)
                     /locus_tag="PLO65_13305"
     CDS             complement(217..534)
                     /locus_tag="PLO65_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRD29270.1"
                     /translation="MRLPRLTSVVLLGLTLAGCATAPAPGTDSGPLGYRCDGGKRFIA
                     AWNQPGDRVRVTAGGVTRSLPHAPSASGARYASGAYEIWGKGDAAMLSGFPGGPYANC
                     RVD"
     gene            complement(543..884)
                     /locus_tag="PLO65_13310"
     CDS             complement(543..884)
                     /locus_tag="PLO65_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRD29271.1"
                     /translation="MRAVLAVLLALSPALPAAAEDGQALFNAQCRSCHNQTGPAGPAL
                     KGVVGRKIATVPGFAYSPGLKAKGGTWTDASLEAYLANPSGFAPGTRMFARVAQPGAR
                     AAIIGYLKTQK"
     gene            1035..1505
                     /locus_tag="PLO65_13315"
     CDS             1035..1505
                     /locus_tag="PLO65_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD29272.1"
                     /translation="MATTLDINGKSVSVTAADDTPLLWVLRDTLGLTGTKYGCGIAQC
                     GACTVHVDGQPVRSCQTPVGAVAGHKVTTIEGLGGHHPVQQAWVAADVPQCGYCQSGQ
                     IMSAVALLQENRHPSDADIDAAMSGNICRCGAYQRIRAAIKDAAGIAAALPAKA"
     gene            1517..>3336
                     /locus_tag="PLO65_13320"
     CDS             1517..>3336
                     /locus_tag="PLO65_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD29273.1"
                     /translation="MNAHSKIPAPSRRDLVVGASLVGGALLVGCSPADLLSIGSKVDV
                     GAFGPFIRIAADGAVTVISKHIEFGQGSHAGLAAIVAEELDADWTRVSVEQAPANAKL
                     YSNSAMGAQGTGGSTAIANSWEQLRKAGAGARAMFVQAAAGKWNVPAGELTVRDSVVS
                     HAKSGRSAGFGELLAAAAKIKPPESPTLKDPKTFTLIGSDRVRRKDSLAKSTGTARYT
                     QDVQEPDMLVAMVAHAPRYGATVKGFDATAAKAVPGVVEVYQIPTGVAVVANSTWAAR
                     QGREALKVDWDESKAEKRSSDQIMAEFRAAGAGKGPQDLKWVPFDSHGDASRADGEVF
                     EATYDFPYLAHAAMEPMNAVARVKGNSVSMSFGSQAPTIDQLNAAKIVGTLPGAVKIE
                     TLFAGGSFGRRANFQSDYAAECVHIAKKVGGGRPVKLVWTREDDMRAGYFRPIVHHNL
                     RISLDKDGYPAVWRHRVVTQSIMKGSPIPSKGDLDATAVEGALGSPYLKATPIVDAQL
                     ALPDVGVPVLWWRSVGATHTAFVMEHTIDQLARKAGKDPVAYRKTLYERAGAKRHLAA
                     LNLALEKAGPAPVGLSRGVAVHESFGSVVAEIADVEIADGA"
BASE COUNT          543 a         1183 c         1176 g          434 t
ORIGIN      
        1 gcctggacga acaggtcgtc gccgacgatg aaggccagga tcgcgcccgt gtaggcgccc
       61 gccaggaagg cgttgggccc ttcgaggcgc acgaccacgg ccggagagcc ctcgaacagc
      121 cgcgccttgg cctcggcggc ctcgcccgcg ccggcgatga ccaccttgcg gccggtcagg
      181 gggaagaagg ctggaaaggc gtccaccggg gctccgtcag tccacgcggc aattggcgta
      241 gggaccgccg ggaaagcccg acagcatggc ggcgtcgccc ttgccccaga tctcgtaggc
      301 gccgctggcg tagcgggcgc cggaggccga gggcgcatgc ggcagggatc gggtcacccc
      361 gccggccgtg acccgcaccc tgtctcccgg ctgattccag gcggcgatga agcgcttgcc
      421 gccgtcgcag cgatagccca gcggcccgct gtccgtgccg ggggccggag ccgtggcgca
      481 gcccgccagc gtcaggccca gcagaacgac agaggtcagg cgcggcaggc gcatcggccc
      541 tcctacttct gggtcttcag atagccgatg atcgccgccc gcgcgcccgg ttgcgccaca
      601 cgggcgaaca tccgggtccc cggcgcgaat cccgaggggt tggcgagata ggcctcgagg
      661 ctggcgtcgg tccaggttcc gcccttggcc ttgaggccgg gagaataggc gaagcccgga
      721 accgtggcga tcttgcggcc gacaaccccc ttgagggccg ggcccgccgg acccgtctgg
      781 ttgtgacagc tgcgacactg ggcgttgaac agcgcctggc cgtcctccgc ggctgcgggc
      841 agggcgggcg acagggccag aaggacggcg agtacggcgc gcatggcggg ctccggctgg
      901 ggtttgacaa cgtagaccgg accgcagaaa aattcactgg cgcgcccccg ccttaacagc
      961 gttaagataa ccgcggcccc gggacttgcc agacccgtgg tgaaaacaga acgcagatcg
     1021 gggaacgttc agacatggcc accactctcg acatcaacgg caagtccgtc agcgtcacgg
     1081 cggcggatga cacgcccctg ctgtgggtcc tgcgcgatac cctgggtctg accggcacga
     1141 aatacggctg cggcatcgcc cagtgcggcg cctgcacggt ccacgtggac ggacagccgg
     1201 ttcgctcctg ccagaccccg gtgggcgcgg tcgccggaca caaggtcacc accatcgagg
     1261 ggctcggcgg ccaccatccc gtacagcagg cctgggtcgc ggcggacgtg ccgcagtgcg
     1321 gctactgcca gtccggccag atcatgagcg ctgtcgccct gctgcaggaa aaccgccatc
     1381 cctcggacgc cgacatcgac gcggccatga gcggcaacat ctgtcgctgt ggcgcctacc
     1441 aacgcatccg ggcggccatc aaggacgcgg ccggcatcgc cgccgccctg ccggccaagg
     1501 cgtgagggag ttcgccatga acgcgcacag caaaatcccg gccccctcgc ggcgcgacct
     1561 ggtggtcggc gccagcctcg tcggcggcgc cctgctggtc ggctgctcgc cggccgacct
     1621 gctcagcatc ggcagcaagg tcgacgtcgg cgccttcggg ccattcatca ggatcgccgc
     1681 cgacggggcg gtgacggtga tctccaagca tatcgagttc ggccagggca gccacgccgg
     1741 cctggcggcc atcgtcgccg aggagctgga cgccgactgg acccgcgtct ccgtcgaaca
     1801 ggctcccgcc aacgccaagc tctattccaa ctcggccatg ggcgcccagg gcaccggcgg
     1861 ctcgaccgcc atcgccaaca gctgggagca gttgcgcaag gccggcgccg gcgcccgggc
     1921 gatgttcgtc caggcggcgg ccggcaagtg gaacgtcccg gccggcgagc tgaccgtgcg
     1981 cgacagcgtc gtcagccatg cgaagagcgg tcggtcggcc ggcttcggcg agttgctggc
     2041 cgcggccgcg aagatcaagc cgcccgaaag cccgacgctc aaggatccga agaccttcac
     2101 cctgatcggc agcgaccggg tccgccgcaa ggacagcctg gccaagagca ccgggacggc
     2161 ccgctacacc caggacgtcc aggagccgga catgctggtg gcgatggtcg cccacgcccc
     2221 gcggtacggg gcgaccgtga agggcttcga cgcgacggcg gcgaaggccg tgccgggcgt
     2281 cgtcgaggtc taccagatcc cgaccggcgt ggcggtggtg gcgaattcca cctgggccgc
     2341 ccgccagggc cgcgaggcgc tgaaggtcga ctgggacgag tcgaaggccg agaaacgcag
     2401 ctccgaccag atcatggccg agttccgcgc ggccggcgcc ggcaaggggc cccaggatct
     2461 caaatgggtt ccgttcgact cgcatggcga cgccagccgg gccgacggcg aggtgttcga
     2521 ggcgacctac gacttcccct acctggccca cgcggcgatg gagccgatga acgcggtggc
     2581 ccgggtcaag ggcaacagcg tcagcatgag cttcggctcc caggccccga ccatcgacca
     2641 gctcaacgcc gccaagatcg tcggcaccct gcccggcgcg gtgaagatcg agaccctgtt
     2701 cgccggcggc tcgttcggcc ggcgggccaa cttccagtcc gactatgccg ccgagtgcgt
     2761 gcacatcgcc aagaaggtcg gcggcggccg gccggtgaag ctggtctgga cccgcgagga
     2821 cgacatgcgg gccggctact tccgcccgat cgtgcaccac aacctgcgta tctcgctcga
     2881 caaggacggc tatccggccg tctggcgcca tcgggtggtc acccaatcga tcatgaaggg
     2941 ctcgccgatc ccgtccaagg gcgacctgga cgccacggcg gtcgagggcg cgctcggctc
     3001 gccctatctg aaggccacgc cgatcgtcga cgcccagctt gcgctgcccg acgtcggcgt
     3061 gccggtgctg tggtggcggt cggtcggcgc gacccacacc gccttcgtca tggagcacac
     3121 catcgaccag ctggcccgca aggccggcaa ggacccggtg gcgtaccgca agaccctcta
     3181 cgaacgggcc ggggccaaac gacacctggc cgcgctgaat ctcgccctcg agaaggccgg
     3241 acccgcgccg gtcgggctgt cgcgcggcgt cgcggtgcat gagtcgttcg gctcggtggt
     3301 cgccgagatc gccgatgtcg agatcgccga cggcgc
//
LOCUS       DAORNX010000346         3328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21626_length_3328_cov_5.867705, whole genome shotgun sequence.
ACCESSION   DAORNX010000346 DAORNX010000000
VERSION     DAORNX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21626_length_3328_cov_5.867705"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>260
                     /locus_tag="PLO65_13325"
     CDS             <1..>260
                     /locus_tag="PLO65_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRD29274.1"
                     /translation="VATRPRIAVLATGEELAAPGTEAGPFQIYESGGAGLLPMIRRWG
                     GLPARLAPAGDSLEAIIAAVRNVEAEVIVTVGGASVGDYDLV"
     assembly_gap    261..388
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..696
                     /locus_tag="PLO65_13330"
     CDS             <389..696
                     /locus_tag="PLO65_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HRD29275.1"
                     /translation="NPASAFVCAELFLRPLILALQGASQGFSLEAARLAEPLPANGPR
                     EHWMRARLGPDGATAFADQDSSLVTVFAAADALLRRPPGAPAAPAGETADILRLDRA"
     gene            810..2615
                     /locus_tag="PLO65_13335"
     CDS             810..2615
                     /locus_tag="PLO65_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29276.1"
                     /translation="MAGQTLIAHALKLDGYIQHYRGMAPVRFAMALGLTGLLATVAPL
                     TFTIGFGVLHLALYVCLSWAVEVAARSPDARAALRDLTIRTGLIVFLLSLHACWMAMV
                     VRGAATTSVLRTETGLLIIGVLMFTALQVHMSRLGYIAAILPSIGAMAWIAIDQRQQM
                     PSAHFGGVVVLFVAAVVAASWRQQATDRTLWNVRLALEEKNAALTALVAEAEAARSQA
                     EAANRAKSDFLAMTSHEIRTPLNAVLGLSEALHRSRLNRRQKAMAAGVLDAGALLKRL
                     LDAVLDITRIEAGKMTVEAAPIDLRRMAATVVSIWTPRASERGVTLVLDIDGLTAPCG
                     VLTDGAKIEQALVNLLSNAVKFSPRGGTVTVRLAATQDDETLTVTAEVLDQGNGVPTG
                     DRLRIFETFEQTSEGQALGGAGLGLAICAGNMALIGGTISVGDAPGGGAAFRFAFAAP
                     AAEVDIGGPVDRSAPTLDETRPLRVLAAEDNAANRHVLRVLLEPLAVVLTLVENGREA
                     VEAMARESFDIVLMDANMPVMDGLAALRAIRAGGGPAALTPVWMLTANVFEEDVVRYR
                     AAGADGVVRKPIDLNELFAALTDAAERVEPEAVAA"
     gene            complement(2618..>3328)
                     /locus_tag="PLO65_13340"
     CDS             complement(2618..>3328)
                     /locus_tag="PLO65_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRD29277.1"
                     /translation="RAGATSLQEIARRSGLSRRSLELLAEADAFRSVGLPRREALWAV
                     KGLAIEVHAERDAPLLAGLPLFEARADLPVMSLPQHVAEDYRTAGLSLKAHPCTFFRP
                     MLDEMGCVRAADLPRMKTGRRVSVAGLVLIRQRPGTAKGIVFITLEDETGPANAVVWK
                     DVFTANRRLAMSSAFLIVHGRIQRDEDGGKEGRVIHIVAERFTDLTPRLAGLREEGQQ
                     PRPGEAIRSRLVRSRDFH"
BASE COUNT          433 a         1164 c         1130 g          473 t
ORIGIN      
        1 gggtcgcgac acggcctcgc atcgccgtgc tggcgaccgg cgaggagctg gccgcgccgg
       61 ggacagaggc cggtccgttc cagatctatg agtctggcgg cgcgggcctg ctgccgatga
      121 tccggcgctg gggcggcctg cccgcccgcc tcgccccggc cggcgactcg ctcgaggcga
      181 tcatcgccgc cgtgcggaac gtcgaggccg aggtgatcgt cacggtcggc ggcgcctcgg
      241 tcggcgacta cgacctggtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aatccggcct cggccttcgt ctgcgccgaa
      421 ctgttcctga ggccgctgat cctcgccctg cagggcgcct cgcagggctt ctcgctcgaa
      481 gcggcgcgcc tggcggagcc cctgcccgcc aacggcccgc gcgaacactg gatgcgcgcc
      541 cggctcggcc ctgacggggc gaccgccttc gccgatcagg attcctcgct ggtgacggtg
      601 ttcgccgccg cggacgccct gctgcgtcgc ccgcccggag ccccggcggc cccggcgggc
      661 gagaccgccg acattctcag gctggatcgg gcgtagtccg aggacggcac gaattcctgt
      721 cggccatttt ggcgatcagc gtcagggtca tccgccctgc ctgtccggct taggccgata
      781 ttcacaaacc tgtggtcgat tgccgcacca tggcaggcca gaccctcatc gcgcatgcgc
      841 tcaagctgga cggctacatc cagcactacc ggggcatggc ccccgtccgg ttcgccatgg
      901 ccctcggcct gaccggcctg ctggcgaccg tcgcgccgct gaccttcacg atcggcttcg
      961 gggttctgca cctggccctc tacgtctgcc tgtcctgggc ggtcgaggtg gcggcgcgca
     1021 gtcctgacgc gcgggcggcg ctgcgagacc tgacgatccg gacaggcctg atcgtcttcc
     1081 tgctgtcgtt gcatgcctgc tggatggcga tggtcgttcg cggcgcggcc accaccagcg
     1141 tgctgcgcac ggaaaccggc ctgctgatca tcggcgttct gatgttcacc gcgctgcagg
     1201 tgcatatgag ccggctgggc tacatcgccg ccatcctgcc ctcgatcggg gcgatggcct
     1261 ggatcgccat cgaccagcgt cagcagatgc cgagcgccca cttcggcggc gtcgtggtgc
     1321 tgttcgtcgc cgcggtcgtc gccgccagct ggcgccagca ggccaccgac cgcacgctgt
     1381 ggaatgtgcg ccttgcgctc gaggagaaga acgccgccct gacggccctc gtggccgagg
     1441 cggaagccgc tcgcagccag gccgaggccg ccaaccgggc caagtccgat tttctcgcca
     1501 tgaccagcca tgagatccgc acaccgctga atgcggtcct ggggctgtcc gaggccctgc
     1561 atcgctcgcg gctcaacagg cgacagaagg ccatggccgc cggcgttctg gatgccggcg
     1621 ccctgctcaa gcggctgctc gacgcggtgc tcgacatcac ccgcatcgag gccggcaaga
     1681 tgaccgtcga ggccgcgccg atcgatctgc gtcgcatggc cgccaccgtc gtgagcatct
     1741 ggacgccgcg cgccagcgag cgtggcgtga cccttgtcct cgacatcgac gggctcaccg
     1801 ccccctgtgg cgtgctgacc gacggggcca agatcgaaca ggcgctggtc aacctgctgt
     1861 ccaacgcggt caagttcagc ccccgtggcg gaaccgtgac ggtgcgactg gcggcgacgc
     1921 aggacgacga gaccctgacg gtgacggccg aggtgctgga ccagggcaac ggcgtgccga
     1981 ccggggaccg tctgcgcatc ttcgagacct tcgaacagac cagcgagggg caggccctcg
     2041 gcggcgccgg cctgggcctg gcgatctgcg ccggcaacat ggccctgatc ggcgggacca
     2101 tttccgtcgg cgacgcgccg ggcggcggcg cggccttccg cttcgccttt gccgcgcccg
     2161 cggccgaggt cgatatcgga gggccggtcg accggtcagc gccgacgctc gacgagacgc
     2221 ggccgctgcg cgtgctggcc gccgaggaca acgccgccaa ccgccacgtc ctgcgggtgc
     2281 tgctcgaacc gttggcggtc gtgctgacgc tggtcgagaa cggtcgcgag gcagtcgagg
     2341 cgatggcgcg cgaatccttc gacatcgtgc tgatggacgc caacatgccg gtgatggacg
     2401 gcctggccgc cctgcgggcg atccgcgccg gcggcgggcc ggccgccctc accccggtgt
     2461 ggatgctgac cgccaacgtg ttcgaggagg acgtggtccg ctatcgcgcg gccggggccg
     2521 acggggtggt gcgcaagccg atcgacctca atgagctgtt cgccgccctg accgacgccg
     2581 ccgaaagggt cgagccggaa gcggtcgccg cctgatctca gtggaagtcc cggctacgca
     2641 cgagccggct gcggatcgcc tcgccggggc ggggctgctg tccctcctcg cgcaggccgg
     2701 ccagccgggg cgtcaggtcg gtgaaccgct cggcgacgat gtggatcacc cggccctcct
     2761 tgccgccgtc ctcgtcgcgc tggatgcggc cgtgcacgat cagaaaggcc gagctcatgg
     2821 ccagccgccg gttggccgta aagacgtcct tccagaccac ggcgttggcc gggccggtct
     2881 cgtcctccag ggtgatgaac acgatgccct tggccgtgcc gggccgctgg cggatcagca
     2941 ccagcccggc cacggaaacc cgccggccgg tcttcatcct cggcaggtca gcggcccgga
     3001 cacagcccat ctcgtccagc atcggccgga agaacgtgca ggggtgggcc ttcagcgaca
     3061 ggccggcggt gcggtagtcc tcggccacat gctgcggcag gctcatcacc ggcaggtcgg
     3121 cgcgggcctc gaacagcggc aggccggcca gcagcggcgc gtcgcgctcg gcatggacct
     3181 cgatggccag ccccttgacc gcccacaggg cctcgcgccg cggcaggccc accgagcgga
     3241 aggcgtcggc ctcggccagc agctccagcg accgccgcga caggcctgac cgccgggcga
     3301 tctcctgcag gctggtcgcc ccggcgcg
//
LOCUS       DAORNX010000347         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21675_length_3322_cov_7.215182, whole genome shotgun sequence.
ACCESSION   DAORNX010000347 DAORNX010000000
VERSION     DAORNX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21675_length_3322_cov_7.215182"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLO65_13345"
     CDS             <1..456
                     /locus_tag="PLO65_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD29278.1"
                     /translation="PNVFAGYWNMPEKTAAEFRPDGFFITGDLGVIDSAGYVSIVGRG
                     KDLIISGGFNVYPKEVEAELDALPGVVESAVIGLPHPDLGEGVTAVVATGGKGAFDEA
                     AVIAALKDRLARFKQPRRVLFVDDLPRNAMGKVQKALLRQIHAGLYKGG"
     gene            complement(453..866)
                     /locus_tag="PLO65_13350"
     CDS             complement(453..866)
                     /locus_tag="PLO65_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29279.1"
                     /translation="MRTITSGALACFLAAGLASAREIPPPPPLVDYSPAAYVGASYVA
                     VCAPARTYVLSISAPNGAGPQLSAFFVRGTPVVAEELAQINGKLTKLKSFPSLKVECL
                     TGHDLISIDGWTAEATGGRLGRVTLHLTDAGVTVD"
     gene            1262..1987
                     /locus_tag="PLO65_13355"
     CDS             1262..1987
                     /locus_tag="PLO65_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29280.1"
                     /translation="MSKKVVLVIGAGDATGGAIARRFAREGYAAVIVRRDADKLTELA
                     GQIRADGGEVHPFGVDARKEDQIVDLFAKVEAGIGPLEAVVFNIGANVNFPIVETTAR
                     VYTKVWEMAAFAGFLTGREAAKYMLPRGRGTILFTGATASLRGGKGFSAFAGAKFALR
                     ALAQSMARELGPQGIHVAHPIIDGAIDTAWIAENFPQRYALKDQDGILNPDHIADAYW
                     ALHSQPRDAWTHELDLRPWMETF"
     gene            1987..2577
                     /locus_tag="PLO65_13360"
     CDS             1987..2577
                     /locus_tag="PLO65_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRD29281.1"
                     /translation="MSKTLEFLFDVGSPTTYLAHKRMPGLIERTGAEVIYVPVLLGGI
                     FKATGNASPVMVPAKGVYMGADMARFAKKFGVTLNMNPHFPINTLPMMRMLAGLVGED
                     PFPRVVDTLFDAMWKGRKQMGDPAVVDEVLTKAGFDAAALMALAETPGAKDALKTNTE
                     AAIARGAFGAPTFFVDHEMFFGQDRLDWVEEALLAS"
     gene            complement(2708..3073)
                     /locus_tag="PLO65_13365"
     CDS             complement(2708..3073)
                     /locus_tag="PLO65_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD29282.1"
                     /translation="MALKARCHCGATQFEIDSAPEEVSSCNCSFCAKRGGLWAYYKPA
                     QFRLLTARDRVSTYQWGHYLGQHHHCAICGCGTWSEFPSFESGQPDFNDPRISINARL
                     IEDFDLDAVPVKKVNGKEY"
BASE COUNT          591 a         1062 c         1125 g          544 t
ORIGIN      
        1 cccaacgtct tcgcgggcta ctggaacatg ccggagaaga ccgccgccga gttccgaccg
       61 gacgggttct tcatcaccgg ggacctcggc gtgatcgact ccgccgggta cgtctccatc
      121 gtcggccgcg gcaaggacct gatcatctcc ggcggcttca acgtctatcc caaggaggtc
      181 gaggcggagc tcgacgccct gccgggggtg gtcgagagcg cggtcatcgg cctgccgcac
      241 ccggacctgg gcgaaggcgt caccgccgtg gtcgccacgg gcggcaaggg cgcgttcgac
      301 gaggccgctg tgatcgccgc gctcaaggac cggctggcgc ggttcaagca gcccaggcgg
      361 gtgctgttcg tcgacgacct gccgcgcaac gccatgggca aggtgcagaa ggccctgctg
      421 cggcagatcc acgccgggct ttacaaggga ggctagtcga cggtgacgcc ggcgtcggtc
      481 agatgcagcg tgacgcgccc cagccgccca cccgtagcct ctgccgtcca gccgtcgatc
      541 gaaatcaggt cgtgaccggt caaacactca accttcagcg aggggaacga cttcagtttc
      601 gtgagcttcc cgttgatctg ggcgagttcc tccgccacga caggtgtccc gcggacaaaa
      661 aaggccgaca gctgcggtcc ggcgccgttg ggagccgaga ttgaaaggac gtaggtccgg
      721 gccggtgcac agacggccac atagctggcg ccaacgtatg ccgcaggcga gtagtccacg
      781 agcggtggcg gtggcgggat ttcgcgggcc gatgcaagtc ccgctgcaag gaagcacgcc
      841 agtgcgccac tggtgatcgt tcgcatatca gcctcgctga gaatcaaggg cagcccggct
      901 cggcggcccc atttgccttg gcgtccgtct gcttttcgag ttttgactct acgtagctac
      961 aagggtcggg ctccgggttc tttccgccgc cagtggaagc ggcatctccc tcaagaaatt
     1021 cgtcaggcaa gggatagcta ggacctaggt ggccgcctcg cgcgataatc cggtttgcga
     1081 tgacaacgag ttcggagact tcgggaggct cttcgggcat tgaaacctct gcgccggcgg
     1141 gaaggtgcag cgaaaagtag cagagcgcat attgcgcgca tagagggaat ttgcccgcgc
     1201 ttgctcgaag atggcagaga agttagttgc tattaaagac taaagaacgg aaagcgtcgc
     1261 catgtcgaag aaggtcgtgc tggtcatcgg ggcgggagac gccaccggcg gcgccatcgc
     1321 ccgtcgcttc gcccgtgagg gttacgccgc cgtcatcgtc cgccgcgacg ccgacaagct
     1381 gaccgaactg gcaggccaga tccgggccga cggtggtgag gtccacccgt tcggggtcga
     1441 cgcccgcaag gaagaccaga tcgtcgacct gttcgcgaag gtcgaggccg ggatcggccc
     1501 gctcgaggcg gtggtattca acatcggcgc gaacgtgaac ttcccgatcg tcgagaccac
     1561 ggcccgggtc tacaccaagg tctgggaaat ggccgcgttc gccggcttcc tgaccggacg
     1621 cgaggcggcg aaatacatgc tgccgcgcgg ccggggcacg atcctgttca ccggcgccac
     1681 cgcgtccttg cgcggcggca agggcttctc ggccttcgcc ggggccaagt tcgcgttgcg
     1741 ggcgctggcc cagtcgatgg ccagggagct ggggccgcag ggcatccatg tggcccaccc
     1801 gatcatcgac ggggccatcg acaccgcctg gatcgccgag aattttcccc agcgatacgc
     1861 gttgaaagac caggacggca tcctcaaccc cgatcacatc gccgacgcct actgggcgct
     1921 gcacagccag ccgcgcgacg cctggaccca tgagctcgac ctgcggccct ggatggagac
     1981 cttctgatga gcaagacgct ggaattcctg ttcgacgtgg gcagcccgac aacctatctg
     2041 gcgcacaagc ggatgccggg cctgatcgaa cggacgggcg cggaggtgat ctatgtgccg
     2101 gtgctgctgg gcggcatctt caaggccacg ggcaacgcct cgcctgtgat ggtgccggcc
     2161 aagggcgtct acatgggcgc cgacatggcc cggttcgcga agaagttcgg cgtgaccctg
     2221 aacatgaacc cgcacttccc gatcaacacc ctgccgatga tgcggatgct ggccggactg
     2281 gtcggcgagg accctttccc ccgggtggtc gatacgctgt tcgacgccat gtggaagggc
     2341 cgcaagcaga tgggcgatcc ggcggtggtc gatgaggtgc tgaccaaggc cggcttcgac
     2401 gccgccgccc tgatggcgct tgccgagacg ccgggggcga aggacgcgct gaagaccaac
     2461 accgaagccg ccatcgcgcg cggcgccttc ggggccccga ccttcttcgt cgatcacgag
     2521 atgttcttcg gccaggaccg cctggactgg gtggaggagg cgctcctcgc gagctgaagc
     2581 agattgcgtg gttcgacacg cgcttcgcgc tgctcaccat gacgtaggag gatcagcttc
     2641 cacgccgcac gtcatcctga gcaggccccg caggggccgt gtcgaaggac gcaagctgtg
     2701 agccaggtca gtactccttc ccattcacct ttttcaccgg cacggcgtcg aggtcgaagt
     2761 cctcgatcag ccgggcgttg atcgagatgc gaggatcgtt gaagtccggc tggccgctct
     2821 cgaaactggg gaactcgctc caggtcccgc agccgcagat ggcgcagtgg tgatgctggc
     2881 ccaggtaatg gccccactgg taggtcgaga cccggtcgcg ggcggtcaga agtctgaact
     2941 gggccggctt gtagtaggcc cacagcccgc cgcgtttggc acagaacgag cagttgcagc
     3001 tggagacctc ctccggagcg ctgtcgattt cgaactgtgt cgcgccgcaa tggcagcggg
     3061 ccttgagagc catggtcgtc tcctgaagat gtgacggcga cggtctaacg cgtcgctgtt
     3121 gtcagcgttc tgtcagcacg ctgtcacgac gggctggaca ccgccgcccg ccctttgcga
     3181 gcctcaggga caggacaggg gagacggcca tgaccgacac catcgaactc gcgcgcgcga
     3241 ccgccggcca gacggccgcc gcgatcgccg ccggccagat cagcgcgctg gaggcgaccg
     3301 acgcggccat cgcgcgcatc ga
//
LOCUS       DAORNX010000348         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_21848_length_3304_cov_6.092644, whole genome shotgun sequence.
ACCESSION   DAORNX010000348 DAORNX010000000
VERSION     DAORNX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21848_length_3304_cov_6.092644"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLO65_13370"
     CDS             complement(<1..754)
                     /locus_tag="PLO65_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRD29283.1"
                     /translation="MERDGVRRGLARAGDWARTRPLHLLPWIVLTAGVVLWPGRRPPG
                     AGLSEDRIMDPAAFDRVEPGRGRAARHPHAIPLIGWRDILWRTVWETVRDKLPSTAGG
                     VTFYVLLAIFPAIGAFVSLYGLVSDVTAVERQLRGLAGVFPASVVQIVGEQMLRLAGQ
                     HQARLSLAFVVSLLLSVWSANAGMKALFEGLNVAYDEREKRNFLRRTALTYASTLGAL
                     VFLSLVALILVAAPVALRRLGVARADDLWIALR"
     gene            828..1592
                     /locus_tag="PLO65_13375"
     CDS             828..1592
                     /locus_tag="PLO65_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRD29284.1"
                     /translation="MKAAVAALYRHPVKGFTPERLAAADLAAGRTFPGDRMYAVEIGD
                     SGFDPDRPAFVPKTRFAVLAHIAGVARARTRHDDSTGVFHASAPGFADFAGDLTGEAG
                     RMAFAAWLADLLGEEARAPLRVLRAPGGWRFTDHPLGHVSIINLASLRDLEARIGRPL
                     DPLRFRANLYVDGWPAWAEQDWQGRTLRIGEATATVFKPIVRCTATHVDPATAERDVE
                     VVKALFDNYGHTFCGLYVQITADGRVAEGDVLELIA"
     gene            1589..2455
                     /gene="prmC"
                     /locus_tag="PLO65_13380"
     CDS             1589..2455
                     /gene="prmC"
                     /locus_tag="PLO65_13380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRD29285.1"
                     /translation="MTTLVKAWKAGGERLKAIGIDQPAIDARLMLEAAAEVSRLEIVT
                     DPYREITAEQEAVYDGYLERRGRREPVSHILGRKGFWKIMLNVNGRVLTPRPDTEVIV
                     DEALKAFPETMAFTLLDLGVGSGAILLAILAERPAARGLGVDISEDALAVARENAANL
                     DLDGRTALLRGDWTAGLGDAGFDLVVANPPYIPSAVIETLEPEVRDHDPRLALDGGPD
                     GLDPYRVLAPEILRVLKPGGMFAVEIGHDQSAAVEALFRQAGADGVRTIKDLGLRDRV
                     VAGSKNPLETGT"
     gene            2688..>3304
                     /locus_tag="PLO65_13385"
     CDS             2688..>3304
                     /locus_tag="PLO65_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HRD29286.1"
                     /translation="MRDFKGMKRQRGRNRGGGGGNQGGKPQHNANRAFDSNGPEGIKV
                     RGAAQSVFEKYQQLARDASSGGDRVLAENYLQHAEHYFRTLRAMQPHRPVSDIVGREQ
                     FASGFDIDFEAEPEEAPEGGYAEQAEAAEPEGQRDSQRFEGRQRDGQQRYEGRDRQRD
                     DRQRDDRPRDDRQREDRPQREDRPREDRPREPREDRPRQDGESTGR"
BASE COUNT          542 a         1195 c         1149 g          418 t
ORIGIN      
        1 agcgcagggc gatccacagg tcgtcggccc gcgccacgcc gagccggcgg agggcgacgg
       61 gcgcggcgac caggatcagg gccaccaggg acaggaacac cagcgccccg agggtcgagg
      121 cgtaggtcag ggcggtgcgc cggaggaagt tgcgtttctc gcgctcgtca taggccacgt
      181 tgaggccctc gaagagcgcc ttcatgccgg cgttggcgct ccacaccgac agcagcaggc
      241 tgacgacgaa ggcaaggctg agcctggcct ggtgctggcc ggccaggcgc agcatctgct
      301 cgccgacgat ctgcaccacg ctggccggga acacgccggc caggccgcgc agctggcgct
      361 ccacggccgt gacgtccgag accaggccgt agagggagac gaaagccccg atggccggaa
      421 agatcgccag caggacgtag aaggtcaccc cgccggcggt gctgggcagc ttgtcccgca
      481 cagtctccca gaccgtccgc cagaggatgt cgcgccagcc gatcagcggg atggcgtgcg
      541 gatgtcgggc ggcccgtccc cgccccggct cgacccggtc aaacgccgcc gggtccatga
      601 tccggtcctc gctgaggccg gcccccggcg gccgccggcc cggccacagg accaccccgg
      661 ccgtcaggac gatccacggc agcaggtgca acggtcgcgt gcgggcccag tcccccgccc
      721 tcgccagtcc cctgcgaacg ccgtctcgct ccatggaggg tgaacaccgc cgccggccgc
      781 tgcgttccgc acttgccccg cctgtcgccg tgaggcactc agcaacgatg aaggccgccg
      841 tcgccgccct gtatcgccat cccgtcaagg gcttcacccc cgagcgcctc gccgccgccg
      901 acctggccgc cgggcgaacg tttcccggcg accggatgta cgccgtggag atcggcgaca
      961 gcggcttcga tcccgatcgc ccggccttcg tgcccaagac ccgcttcgcc gttctggcgc
     1021 acatcgccgg ggtggccagg gctcgcactc gccacgacga ctcgaccggc gtgtttcacg
     1081 cgtccgcgcc cggctttgcc gacttcgccg gcgacctgac cggcgaggcg ggccgcatgg
     1141 ccttcgcggc ctggctggcc gaccttctgg gcgaggaggc ccgggccccc ctgagggtgt
     1201 tgcgggcgcc gggcggctgg cggttcaccg accatccgct gggccatgtc tcgatcatca
     1261 acctggccag cctgcgcgat ctggaggccc ggatcggccg cccgctcgat ccgctgcgct
     1321 tccgcgccaa cctctatgtc gatggctggc cggcctgggc cgagcaggac tggcagggtc
     1381 gcacgctgcg catcggcgag gcgacggcca cggtgttcaa gccgatcgtc cgctgcacgg
     1441 cgacccacgt cgacccggcc acggccgagc gcgacgtcga ggtggtaaag gcgctgttcg
     1501 acaactacgg ccacaccttc tgcggcctct acgtgcagat caccgccgac ggccgggtgg
     1561 ccgagggcga cgtcctggag ctgatcgcgt gaccaccctt gtgaaggcct ggaaggccgg
     1621 cggcgagcgg ctgaaggcca tcggcatcga ccagcccgcc atcgacgcgc ggctgatgct
     1681 ggaggccgcc gcggaggtca gccggctgga gatcgtcacc gacccctacc gcgagatcac
     1741 cgccgagcag gaggccgtct atgacggcta cctcgagcgc cgcggccgcc gtgaaccggt
     1801 cagccatatc cttggccgca agggcttctg gaaaatcatg ctgaacgtca acggcagggt
     1861 cctgaccccc cgtcccgaca ccgaggtgat cgtcgacgag gcgctgaagg cgtttcccga
     1921 gaccatggcc ttcaccctgc tggacctggg cgtcggctcg ggcgccatcc tgctggcgat
     1981 cctcgccgaa cggccagccg cgcgggggct gggggtcgac atttcggagg acgccctggc
     2041 cgtggcgcgg gaaaacgcgg ccaacctcga cctcgacggc cgcaccgccc tgctgcgcgg
     2101 cgactggacg gcgggcctcg gcgacgcggg cttcgacctg gtggtggcca acccgcccta
     2161 catcccaagc gcggtgatcg agacgcttga accggaggtg cgcgaccacg acccgcgcct
     2221 ggccctcgac ggcggcccgg acggactcga tccctaccgc gttctggcgc cggagatcct
     2281 gcgcgtgctc aagccgggcg ggatgttcgc ggtcgagatc ggccacgacc agagcgccgc
     2341 cgtcgaggcc ctgttccgcc aggccggcgc ggacggcgtg cggacgatca aggacctggg
     2401 cctccgcgac cgggttgtcg cgggttccaa aaaccccttg gaaacaggga cctgaggcgt
     2461 tacatctgac tcgtccccac tcagaacgcc gtagagattg atcgcaatcg acggcgggcg
     2521 cgcgaggtcc cacgaggacc gggtcgcggc gctgaaagcc gggcggggac gaagaacagc
     2581 gtctttttcg aaacaaaaaa gcgggcggca agcccgaacc agtatccaga accgacccgc
     2641 gccaccgccc atagcgtcgt gcgccggccg gcaggtcgaa gggtaaaatg agagatttca
     2701 agggtatgaa gcgtcagcgt ggccgcaatc gtggcggcgg cggcggcaac cagggcggca
     2761 agccccagca caacgccaac cgcgcgttcg actccaacgg acccgagggc atcaaggtcc
     2821 gcggcgcggc ccagagcgtg ttcgagaagt accagcagct ggcgcgggac gcctcttccg
     2881 gcggcgaccg ggtgctggcc gagaactacc tgcagcacgc cgagcactat ttccgcaccc
     2941 tgcgggcgat gcagccgcat cgcccggtgt ccgacatcgt cgggcgcgaa cagttcgcct
     3001 ccggctttga catcgatttc gaggccgaac ccgaagaggc gcccgagggc ggctatgccg
     3061 agcaggccga ggccgccgag ccggagggcc agcgcgacag ccagcgcttc gaaggccgtc
     3121 agcgcgacgg gcaacaacgc tatgaaggcc gggatcgcca gcgtgacgac cgccagcgtg
     3181 acgaccggcc ccgcgatgac cgacagcgcg aagaccgccc gcagcgcgaa gaccgtcccc
     3241 gcgaggacag gccccgcgag ccacgcgagg atcgtccgcg ccaggacggc gaatccaccg
     3301 gtcg
//
LOCUS       DAORNX010000349         3213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_22617_length_3213_cov_6.141229, whole genome shotgun sequence.
ACCESSION   DAORNX010000349 DAORNX010000000
VERSION     DAORNX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22617_length_3213_cov_6.141229"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLO65_13390"
     CDS             <1..211
                     /locus_tag="PLO65_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29287.1"
                     /translation="VGSGDAGTGAAIGAVVGGTAGAVKGCREDGGCGATTPTKRQYYD
                     EAAGRYYYYDQASGRYYWEDGRPRN"
     gene            299..733
                     /locus_tag="PLO65_13395"
     CDS             299..733
                     /locus_tag="PLO65_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD29288.1"
                     /translation="MSKLAVLGTVAVMLSGCGTLSRMQRVRTPNVCQDLTVSIYFRRD
                     SAVVTREARAVLRGAGELARGCVLGKVDVTGLADSVGAPEVNLALSKKRADAVRQAVE
                     RLGFTTVNFSVGAVGEQGAVTPAGDDRPLRRRAEVTFHLRPH"
     gene            1024..1260
                     /locus_tag="PLO65_13400"
     CDS             1024..1260
                     /locus_tag="PLO65_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29289.1"
                     /translation="MATFFSRFPRAHETHIEATDARQGRWGRHVFWVLVASTSLAAIT
                     LFASWALRAGDLAAADATSTPTAAETRAAGEPVG"
     gene            complement(1262..2053)
                     /locus_tag="PLO65_13405"
     CDS             complement(1262..2053)
                     /locus_tag="PLO65_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29290.1"
                     /translation="MSLLARLAPHLPYMRRYARALTGSQATGDQYVRVALEALAAGEK
                     TLDNRLSPRVALYHVFHAIWCTSGARLEDRAPVEGHDAGQRLMRIAPRSRQAFLLTAL
                     EGFTPSEAAQILDTDFPEVERLISQAQNEIDAELATDVLIIEDEPVIAADIEALVREL
                     GHAVVDIAATRTEAVEAVARKAPGLVLADIQLADGSSGIDAVKDILGHYDIPVIFITA
                     FPERLLTGERPEPTFLITKPFQPETVKAAIGQALFFHPGRTAKAA"
     gene            2281..2466
                     /locus_tag="PLO65_13410"
     CDS             2281..2466
                     /locus_tag="PLO65_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HRD29291.1"
                     /translation="MIEQSAMDERRKGTAPLDEARLRQRAIGVKLRQMFDEVVNEPVP
                     DEFLDILRRADNKTGES"
     gene            2468..3079
                     /locus_tag="PLO65_13415"
     CDS             2468..3079
                     /locus_tag="PLO65_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD29292.1"
                     /translation="MAAASDAAAAPLEGAARDRVFQRDLVALIPHLRAFARTLTGDAT
                     AADDLAQDAMIKAWDARASFQLGTNMKAWTFMILRNQFYSDKRRSWRSTQLDQEAAER
                     TLVAVDDPGSPIALDELRLAMGMLPLEQREALILVGAGGFAYEEAADICGCAVGTVKS
                     RVSRARKALQGILETGAYDRDGRDAGDAMRAILADAERLSAIR"
BASE COUNT          512 a         1053 c         1172 g          476 t
ORIGIN      
        1 tgtcggcagc ggcgatgcgg gaacgggcgc ggcgatcggc gcggtcgtcg gcggcaccgc
       61 cggggccgtc aagggctgtc gcgaggacgg cggctgcggc gcgaccaccc cgaccaagcg
      121 ccagtactat gacgaggcgg ccggtcggta ctactattac gatcaggcct ccggccgtta
      181 ctactgggaa gatggccggc cgcggaactg acgtcagcgc ctgctgatgg gcggaacggg
      241 cggttggctg tcaggccggc cgcccttttc ttgggctgag ggtagggaaa tgaacagaat
      301 gtcgaagctg gccgtcctcg ggacggtcgc cgtgatgctg agcggttgcg ggaccctgtc
      361 ccggatgcag cgggtcagga cgcccaatgt ctgccaggac ctgaccgtct cgatctattt
      421 ccgccgcgat tcggcggtcg tcacccgcga ggcgcgggcc gtgctgaggg gcgcgggcga
      481 gctggccagg ggctgcgtgc tgggaaaggt cgatgtgacc gggctggccg actcggtcgg
      541 ggcgccggag gtcaatctgg ccctgtcgaa gaagcgcgcc gacgcggtgc ggcaggcggt
      601 cgaacggctg ggcttcacca ccgtcaactt ctcggtcggg gcggtgggcg agcaaggggc
      661 ggtgaccccc gccggcgacg accggcccct gcggcggcgg gccgaggtga ccttccatct
      721 gcgcccgcac tgacgcccgt cggggcctgc agggcggacg caagaaaagg gggcggatcg
      781 caagatccgc ccctcgagtt cgcatcatcg agaaaagggg tgcggaggcg actggctggc
      841 tttcaccagc ctcaagtcgg ggggggcgtc gccgcctccg caatccataa acgtcccgcc
      901 gtgaaattcc gttccgggcc agggcgaaaa agttcgaact tttttcaggc caggcgcgaa
      961 gccggaacaa gcccttggcg ggaaacgttt tcggtccacc aatcggacag gaggcgttac
     1021 gtcatggcca cgtttttcag ccgctttccc cgggcgcacg agacgcatat cgaggcgacc
     1081 gatgcgcggc agggccgctg gggccggcac gtgttctggg ttctggtggc ctcgaccagc
     1141 cttgcggcga tcacgctgtt cgccagctgg gcgttgcggg ccggggacct ggccgcggcc
     1201 gacgcgacca gcacgccgac ggccgccgag acacgggccg ccggcgagcc ggtcggatag
     1261 gtcaggccgc cttggcggtc cggcccggat gaaagaacag cgcctggccg atggccgcct
     1321 tcaccgtctc cggctggaac ggcttggtga ttaggaaggt cggttccggc cgctcgcccg
     1381 tcagaaggcg ttcggggaag gcggtgatga agatcaccgg aatgtcgtag tgaccgagga
     1441 tgtccttgac cgcatcgatg cccgacgatc cgtcggccag ctggatgtcg gccagcacca
     1501 gccccggcgc cttgcgggcg acggcctcga cggcttcggt gcgggtggcg gcgatatcga
     1561 ccacggcatg gccgagttcg cgcaccagcg cctcgatgtc ggccgcgatg accggctcgt
     1621 cctcgatgat cagcacgtcg gtggccagtt ccgcgtcgat ttcgttctgc gcctgggaga
     1681 tcagccgctc gacctcgggg aagtcggtgt cgagaatctg cgcggcctcg ctgggggtga
     1741 agccctcaag cgccgtcagc agaaaggcct gccgcgaccg cggcgcgatg cgcatcaggc
     1801 gctggccggc gtcatggccc tcgaccgggg cgcggtcctc gagccgggcg cccgaggtgc
     1861 accagatggc gtgaaacaca tgatacagcg ccacccgcgg cgacagccgg ttgtccaggg
     1921 tcttctcgcc ggccgccagg gcctcgagcg cgacgcggac atactggtcg ccggtggcct
     1981 ggctgcccgt cagggccctc gcataccggc gcatgtaggg cagatgcggc gccagacgag
     2041 caagaagact cataacccct cccgttttca ggtcttcgga tcgggacacg cgccgccgcc
     2101 cgcaaggacg gggcgcgtct tccacgagac agccaacgcg tcaggatgac aacggttccc
     2161 agattacgcc gtctgggcca tcatggcgaa cacggtttcg ggaaccaggg gcggagacgg
     2221 acgttgaacg gccggagtca cagttccagg agggggcggg catctcgcca cgccgacagc
     2281 atgatcgaac aaagtgccat ggatgaacgg cggaaaggta cggccccgct cgacgaggct
     2341 cgcctgcggc aacgcgcgat cggcgtgaaa ttgcgccaga tgttcgacga ggtggtcaac
     2401 gagcccgtgc cggacgagtt tctcgacatc ttgcggcggg cggacaacaa aaccggtgag
     2461 agctgagatg gcggcagcat cggacgccgc ggccgcgccc ctcgagggcg cggcccgcga
     2521 cagggtgttc cagcgcgacc tggtggcgct gatcccccac ctgcgcgcct tcgcccgcac
     2581 cctgaccggc gacgcgaccg ccgccgacga tctggcgcag gacgccatga tcaaggcctg
     2641 ggacgcccgc gccagcttcc agctgggcac caacatgaag gcctggacct tcatgatcct
     2701 gcgcaaccag ttctattccg acaagcgccg ctcctggcgc tcgacccaac tggaccagga
     2761 ggccgccgaa cggacgctgg tggccgtgga tgatcccgga tccccgatcg ccctcgacga
     2821 gctgcgcctg gccatgggca tgctgccgct ggagcagcgc gaggccctga tcctggtggg
     2881 cgccggcggc ttcgcctacg aagaggccgc cgacatctgc ggctgcgcgg tcggaacggt
     2941 gaagagccgg gtcagccggg cgcgcaaggc cctgcagggc atcctggaga ccggagccta
     3001 tgatcgcgac ggccgggacg ccggcgacgc catgcgcgcg atccttgccg acgcggagcg
     3061 gctgagcgcg atccggtgaa attgctcggc cggttgcggt gggggcccgc gaccacgatc
     3121 cgcgtgcgcc ttggcgtcgc catggcggcg gcgttgctgc ccgtcctgct gctcggggcc
     3181 gggcagtccc tgctggcctt ccagcgcgag gca
//
LOCUS       DAORNX010000350         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23105_length_3165_cov_6.401286, whole genome shotgun sequence.
ACCESSION   DAORNX010000350 DAORNX010000000
VERSION     DAORNX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23105_length_3165_cov_6.401286"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..861)
                     /locus_tag="PLO65_13420"
     CDS             complement(100..861)
                     /locus_tag="PLO65_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRD29293.1"
                     /translation="MSPDRVTAAVMIIGDEILSGRTQDTNLAAIAKYIGTYGVDLAEA
                     RVVPDDEDEIVAALNHLRTKYDYVITTGGIGPTHDDITADCVAKAFGVALYEHPEIIA
                     MMESRWKTELNAARRRMARVPEGGSLVKNPVQGPPGFQIENVFVLAGVPSIMRGMLED
                     VGPRLRGGAVVISRTLRVDGSGEGAIAAPLEAVAKAHPDLSLGSYPFFSNEGYGSNLV
                     IRGRDQAEVESTLAELSAALLDAGITGLTRLDTQP"
     gene            986..1702
                     /gene="sfsA"
                     /locus_tag="PLO65_13425"
     CDS             986..1702
                     /gene="sfsA"
                     /locus_tag="PLO65_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRD29294.1"
                     /translation="MDFPSPLARGRLVSRYKRFFADVLLDDGAEVTAHCPNPGAMLGL
                     NTPGLGVWLSRSDAPKRKLAHTLELVEADGGLVGVNTMLPNRLVAEALAADVIPELAG
                     YAVHRREVKYGENSRVDFLLTHPDRPACWLEVKNCHMRRAGTLAEFPDCVAARSTKHL
                     RELAGRVRAGDRAVALFVIQRTDCDAFSACAELDPAFAAALDEVVDEGVEALCYACDM
                     SPRRIAIARRLPRVRAVSRA"
     gene            complement(1689..2864)
                     /locus_tag="PLO65_13430"
     CDS             complement(1689..2864)
                     /locus_tag="PLO65_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29295.1"
                     /translation="MPRTVWALGLVSMFTDVSSEIIHSLLPVFLVTGLGASVALVGLI
                     EGVAEATASITKLFSGWISDRLGKRKLLVLIGYGLATLTKPVFPLAVTPFEVMAARFA
                     DRIGKGIRGAPRDALIADITPNGGRGAAYGLRQSLDTVGAFVGPLIAMALMVVLAGDM
                     RAVFGWAVVPAMIAVVLIIIGVEEPRGVPRSADGAPPIRLTDVRGLGRRFWGIVLLGV
                     VFTLARFSEAFLILRAQGVGLPIALAPVVLVLMNVVYAGVAAPVGALSDRIGRRSLLA
                     TGLVMLLAADLVLTLFGSVAGVLVGVALWGLHLGLTQGLLATLVADAAPAPLRATAFG
                     LFNLATGVSLLAASVIAGLLWAALGAQATFLAGAGFTLLALTGLLLFGRPPATVRPD"
     gene            3047..>3165
                     /locus_tag="PLO65_13435"
     CDS             3047..>3165
                     /locus_tag="PLO65_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRD29296.1"
                     /translation="MTLTDAISDAEYRTGEIRIHGAEGFEGMRKAGRLAAECL"
BASE COUNT          526 a         1106 c         1080 g          453 t
ORIGIN      
        1 atgccggcga ggccagcggt gagagcgaga acaacggaaa cacggcgcat gacgcgacct
       61 cgttactgga caggagaaat caccccaaat tgggggcgcc taaggttggg tgtcaagccg
      121 ggtcaacccg gtgatgcccg cgtcgagaag ggcggctgac agctcggcaa gggtgctttc
      181 aacctccgcc tgatcgcgtc cgcgaatgac caggttcgag ccgtaacctt cgttggagaa
      241 gaagggatag ctgcccagcg acaggtccgg atgggccttc gccacggcct cgagcggcgc
      301 ggcgatcgcg ccctcgccgg aaccgtcgac ccgcagggtg cgtgaaatca ccacagcccc
      361 gcccctcaac cgcggcccca cgtcctcgag catgccgcgc atgatcgacg gaacgccggc
      421 gagcacgaag acattctcga tctggaagcc gggcgggccc tggactgggt tcttgaccag
      481 gcttccgccc tccggcaccc gggccatgcg ccgccgggcc gcgttgagct cggtcttcca
      541 gcgcgattcc atcatcgcga tgatctccgg gtgctcgtac agcgcgaccc cgaaggcctt
      601 ggcgacgcag tcggcggtga tgtcatcatg cgtcggaccg atgccgccgg tggtgatgac
      661 atagtcgtac ttcgtccgga ggtgattcag cgccgcaacg atctcgtctt cgtcgtcggg
      721 cacgacacgc gcctcggcca ggtcgacgcc ataggtcccg atatatttcg cgatggccgc
      781 caggttggtg tcctgggtgc ggcccgagag gatctcgtcg ccgatgatca tcacggcggc
      841 tgtgactcgg tccgggctca tcggtgactc cgcttcgtcc gctcaggcgc ggcgtttgag
      901 ggtgttctag cgtctggcgc ggggttgtca ggaaaaaagg ccattcccgc tttcgcgctg
      961 cggtccgttg acctaggtcg ttgcgatgga ctttccatcc ccgctcgccc gtggccgcct
     1021 ggtcagccgc tacaagcggt tcttcgccga cgtgctgctc gacgacggcg cggaggtcac
     1081 ggcccattgc ccgaatcccg gcgccatgct cggcctcaac acgcccggcc tgggcgtctg
     1141 gctgtcacgg tcggacgccc cgaagcgcaa gctggcccac acgctcgaac tggtcgaggc
     1201 cgacggcgga ctggtcgggg tcaacaccat gctgcccaat cggctggtcg ccgaggccct
     1261 ggcggcggac gtcattcctg aactggccgg ctatgcggtt caccggcgcg aggtgaagta
     1321 tggcgagaac agccgggtcg atttcctgct gacccatccg gaccggcccg cctgctggct
     1381 ggaggtaaag aactgccaca tgcgtcgggc cgggaccctg gccgagttcc ccgattgcgt
     1441 ggccgcacgg tcgacgaaac atttgcggga gctggccggg cgggtccgcg ccggcgaccg
     1501 ggccgtggcc ctgttcgtga tccagcgcac ggattgcgac gccttctccg cctgcgccga
     1561 gctcgatcct gccttcgccg cggcgctgga cgaggtcgta gacgagggcg tcgaggcgct
     1621 ctgctacgcc tgcgacatga gcccgcgccg gatcgcgatc gcccgccgcc ttccgcgggt
     1681 ccgcgcggtc agtcgggcct gaccgtcgcc ggcggccggc cgaacagcag cagcccggtg
     1741 agcgccagca gcgtgaaccc ggccccggcc aggaaggtcg cctgggctcc gagcgcggcc
     1801 cacagcaggc cggcgatgac gctggccgcc agcagcgaga cgccggtcgc caggttgaac
     1861 aggccaaagg cggtggcccg caacggggcc ggcgcggcgt cggccaccag cgtcgccaga
     1921 aggccctggg tcaggcccag atgcagtccc cacagggcga cgccgacgag cacgcccgcc
     1981 acgctgccga acagggtcag caccagatcg gccgcgagca gcatgaccag gccggtggcg
     2041 agcaaactgc gccggccgat gcggtccgac agggctccca ccggggcggc gaccccggcg
     2101 tagaccacgt tcatcaacac cagcaccacg ggcgccaggg cgatcggcag gcccacgccc
     2161 tgggcccgca ggatcaggaa ggcctcgctg aaccgggcca gggtgaagac cacgcccagc
     2221 agcacaatgc cccagaaccg gcgccccagg ccacgcacgt cggtcagccg gatcggcgga
     2281 gcgccgtccg cgctccgggg gacgccgcgc ggctcctcga cgccgatgat gatcaggacc
     2341 accgcgatca tcgccggcac gaccgcccag ccgaacaccg cccgcatgtc gcccgccagc
     2401 acgaccatca gcgccatggc gatcagcggc ccgacgaagg cgccgacggt gtccagggac
     2461 tgcctcaggc cataggccgc gccgcgcccc ccgttgggcg tgatgtcagc gatcagggcg
     2521 tccctcggcg cgccgcgaat gcccttgccg atgcggtctg cgaagcgtgc ggccataacc
     2581 tcgaacggtg tgaccgcgag cgggaagacc ggtttggtca gggtcgcaag accgtagccg
     2641 atcagcacca gcagcttgcg cttgcccaac cggtcgctga tccagcccga gaacagcttg
     2701 gtgatcgagg ccgtcgcctc ggccacgccc tcgatcaggc cgaccagggc cacgctggcc
     2761 ccgaggcccg tcaccaggaa caccggaagc aggctgtgga tgatctccga cgagacgtcg
     2821 gtgaacatcg acaccagccc gagcgcccag acggtgcggg gcacagcttt caatccggtc
     2881 ctaggggcgg tcatgggcgg ggcgtttagc gcgacgcccg gctgacggcc agtcgccggg
     2941 cttctcggga gcgatcattg aacttttgtg atagggttgc gacataggac ggaccgacac
     3001 gcgcatcgtc ccccggattt tccggcccgg acgaaaggac cgattgatga ccctgaccga
     3061 cgccatttcc gacgcggagt accgcaccgg cgagatccgc atccatggcg ccgagggctt
     3121 cgagggcatg cgcaaggccg gccggctggc cgccgaatgc ctcga
//
LOCUS       DAORNX010000351         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23136_length_3162_cov_7.256196, whole genome shotgun sequence.
ACCESSION   DAORNX010000351 DAORNX010000000
VERSION     DAORNX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_3162_cov_7.256196"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PLO65_13440"
     CDS             complement(<1..1212)
                     /locus_tag="PLO65_13440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD29297.1"
                     /translation="MDAQSQWTGLNAGRLERITDHIERNYIQPGKIAGCQVEVRRHGH
                     QAYARSFGLRDRERGVAWSDDTIVRIYSMTKPIVSVALMTLFEQGRFELDDPVHRFIP
                     EWKNHRVWVSGEGAGMVTVAPDRPVSFRDVLSHTAGLTYGSMLAHLTGLPIEHPVDKA
                     YDEVGVRRGEGETLDQFLQKLGEVPLRYQPGQRWMYSLATDVCGGLVERVSGMPLEDY
                     LKQAIFEPLGMVDTAFHVPADKVGRFAANYMRLPDKSLRLSDDPTNSTYLRDPVFKSG
                     GGGLVGTMADYVRFAEMLRAGGALDGARVIGPRTLDLMTKNHLKDGQDLTQAAIGGFS
                     ETANAGIGFGLGFAMTIDQTLTGGLSSGDFYWGGAASTIFWADPKEDISVVFMTQLMP
                     SATFNFRGQLKS"
     gene            complement(1290..1652)
                     /locus_tag="PLO65_13445"
     CDS             complement(1290..1652)
                     /locus_tag="PLO65_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29298.1"
                     /translation="MKFLSATAVVLLLLAPAAAAARAPACDVTVTFSSLAMGIDTGAF
                     ARVERYVARHRRLVSAATTTAWGHEGERTLCLTTPSRRAATTVFRDIRDLVGKGRRGP
                     VEVRTLDGRVWQSRPNAR"
     gene            1780..2691
                     /locus_tag="PLO65_13450"
     CDS             1780..2691
                     /locus_tag="PLO65_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRD29299.1"
                     /translation="MRRLAVAALAICLASSPALAAPGDHALDDVRILSADDMGGRGIE
                     TEGSARARAYIAGRFRDLGLAVVEQPFGFTRGSDGKEVHGVNLIVRIEGSRPGGKVLV
                     VTAHYDHLGTRGGVIYNGADDNASGVAGLLAVAEAFTATPPVHTVILVALDGEETGLR
                     GARAFVASPAVPLADIGLNVNFDMIAKNAGGEIYASGGSRFPWLRTRIERLAATAPVT
                     LKQGHDSDIDGPHGNWTSQSDQAAFADKGIPWVYFGVEDHPEYHKPTDDFATVPQAFF
                     LGAVATVVAACRLFDAELDAMMDEAGR"
     gene            2692..>3162
                     /locus_tag="PLO65_13455"
     CDS             2692..>3162
                     /locus_tag="PLO65_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRD29300.1"
                     /translation="MHTLIPIAERIGARLVERGESVAVSESSSGGLVSAALLAVPRAS
                     VWYQGASVTYTPNVARGLLGLTRESMPPGVRSSTEPYALFMAGVIREKLRGTWGLCET
                     GAAGPTGNPYGDAAGHTCVGIVGPVEASFTLETGDGDRVANMLAFARFALERFEA"
BASE COUNT          453 a         1083 c         1137 g          489 t
ORIGIN      
        1 gctcttcagc tggccgcgga agttgaacgt cgccgacggc atcagctggg tcatgaagac
       61 caccgagatg tcctccttcg ggtcggccca gaagatcgtc gaggccgcgc cgccccagta
      121 gaagtcgccg ctggacaggc cgccggtcag cgtctggtcg atggtcatgg cgaagcccag
      181 gccaaagccg atgccggcgt tggcggtctc ggagaagccg ccgatcgccg cctgggtcag
      241 gtcctgcccg tccttcaggt ggttcttcgt catcaggtcc agggtgcgcg gtccgatcac
      301 ccgcgcgccg tccagcgcgc ctccggcccg cagcatctcg gcgaaccgca catagtcggc
      361 catcgtgccg accaggccgc cgccgcccga cttgaacacc ggatcccgca gataggtgct
      421 gttggtcggg tcgtcggaca ggcgcaggct cttgtcgggc aggcgcatgt agttggcggc
      481 gaagcggccg accttgtcgg ccggcacgtg gaaggcggta tcgaccatgc ccagcggctc
      541 gaagatcgcc tgtttcagat agtcctccag cggcatgccg ctgacccgct cgaccaggcc
      601 gccgcagacg tcggtggcga ggctgtacat ccagcgctgg ccgggctggt agcgcagggg
      661 gacctcgccc agcttctgca ggaactggtc gagggtctcg ccctcgccgc ggcgcacgcc
      721 gacctcgtca taggccttgt cgaccgggtg ctcgatcggc aggccggtca ggtgggccag
      781 catcgaaccg taggtcaggc cggcggtgtg gctgagcacg tcgcggaagc tgaccggccg
      841 gtccggggcc acggtgacca tgccggcgcc ctcgccggag acccagacgc ggtggttttt
      901 ccactcgggg atgaagcggt ggaccggatc gtcgagttcg aagcggccct gttcgaacag
      961 ggtcatcagc gccaccgaga cgatcggctt ggtcatcgaa tagatgcgca cgatggtgtc
     1021 gtcgctccag gccacgccgc gttcgcggtc gcgcaggccg aagctgcggg cataggcctg
     1081 gtgcccgtgt cgccgcacct cgacctggca gccggcgatc ttgccgggct ggatgtagtt
     1141 gcgctcgatg tggtcggtga tgcgttcaag ccggccggcg ttcaggccgg tccattgcga
     1201 ctgggcgtcc atgcttcgct ccctttgtct cgttgccggc atgatggccg ggcgacgcgg
     1261 gggaggcaaa gcagaaaaag cggtatggcc tatcgggcgt tcggcctcga ctgccagacg
     1321 cggccgtcga gggtgcgcac ctcgaccggg ccgcgacggc ccttgccgac cagatcgcgg
     1381 atgtcgcgga acacggtcgt ggcggcccgt cgcgacgggg tcgtcaggca gagcgtccgt
     1441 tccccctcgt gaccccaggc ggtcgtcgtg gcggctgaga ccaggcggcg atgccgggcg
     1501 acatagcgct cgacccgggc gaaggccccg gtgtcgatgc ccatcgccag gctgctgaag
     1561 gtgacggtga cgtcacaggc cggcgccctg gccgctgcgg cggcaggggc cagcagcagc
     1621 aggacgaccg ctgtggcgga aagaaacttc atgagtcacc cgatcgatgg ggccgatggc
     1681 gagcgacgac cctgcgcctg tccgcctgaa cggcggatga ccggcttgcg ccgggccgtc
     1741 cgtggccgca ttgagggcgt gatgccgtcc ggagagtcca tgcgccgcct cgccgtcgcc
     1801 gccctcgcga tctgcctcgc cagttcgccg gccctggccg cgccgggcga ccatgcgctc
     1861 gacgacgtgc gcatcctgtc ggccgacgac atgggcggcc gcggcatcga gaccgaaggc
     1921 tcggccaggg cgcgagccta tatcgccggc cgtttccgcg atctcgggct ggcggtggtc
     1981 gagcagccgt tcggcttcac ccgcggctcg gacggcaagg aagtccacgg cgtcaatctg
     2041 atcgtccgga tcgaaggctc gcgccccggc ggcaaggtgc tggtggtcac ggcgcactat
     2101 gaccatctgg ggacccgcgg cggggtgatc tacaacggcg cggatgacaa cgcctcgggc
     2161 gtggcgggcc ttctcgcggt ggccgaggcc ttcacggcga cgccgccggt ccacaccgtc
     2221 attctggtgg cgctcgacgg cgaggagacc ggcctgcgcg gcgcccgcgc cttcgtcgct
     2281 tccccggccg tcccgctggc ggacatcggc ctcaacgtca atttcgacat gatcgccaag
     2341 aacgcggggg gcgagatcta cgcctcgggc ggatcgcggt ttccctggct ccggacccgg
     2401 atcgagcgcc tcgccgccac cgcgccggtg accctgaagc agggacacga cagcgacatc
     2461 gacgggccgc atggcaactg gaccagccag tccgaccagg cggccttcgc ggacaagggg
     2521 attccctggg tctacttcgg cgtcgaggac catcccgaat accacaagcc gaccgacgac
     2581 ttcgccacgg tgccgcaggc cttcttcctt ggcgcggtgg cgacggtggt ggcggcctgc
     2641 cgcctgttcg acgccgagct cgacgccatg atggacgagg ctggacgcta gatgcacacc
     2701 ctgattccca tcgccgaacg gatcggcgcc cgcctggtcg aacggggcga gagtgtcgcg
     2761 gtctcggagt cgtcctcggg cggcctggtt tcggcggcgc tgctggccgt gccgagggcc
     2821 tcggtctggt accagggcgc cagcgtgacc tacacgccca acgtggcgcg cgggctgctg
     2881 gggctgacgc gcgagtccat gccgccgggc gtgcgctcct cgaccgagcc ctacgccctg
     2941 ttcatggccg gcgtgatccg cgagaagctg cgcggaacct ggggcctgtg cgagaccggc
     3001 gcggccggcc cgaccggcaa cccctacggc gacgcggcgg gccacacctg cgtggggatc
     3061 gttggccccg tcgaggcgtc attcaccctg gaaaccggcg acggcgaccg cgtcgccaac
     3121 atgctggcct tcgcccggtt cgcgctggag cggttcgagg cg
//
LOCUS       DAORNX010000352         3151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23242_length_3151_cov_5.455426, whole genome shotgun sequence.
ACCESSION   DAORNX010000352 DAORNX010000000
VERSION     DAORNX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23242_length_3151_cov_5.455426"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLO65_13460"
     CDS             complement(<1..885)
                     /locus_tag="PLO65_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD29301.1"
                     /translation="MSANADLLTRRLAAVPRGVATATAIFAARAENAELWDVEGNRYV
                     DFAGGIAVLNTGHRHPKVLAAVRAQLDAYTHTAFQVVAYEPYIALAERLNALAPFEGP
                     ARTILFTTGAEAVENAVKIARAATGRSAVIAFTGGFHGRTMLTMAMTGKVAPYKRLFG
                     PLPAEVFHAPFPIPQYGVSVEDSLRALDFLFRADVEPERVAAIVIEPVQGEGGFQPAP
                     PELLRALREICDRHGILLISDEVQAGFARTGRMFGIEHSGVQPDLVTVAKSLAGGFPL
                     SGVIGRAAIMDAAEPGGLG"
     gene            complement(951..2147)
                     /locus_tag="PLO65_13465"
     CDS             complement(951..2147)
                     /locus_tag="PLO65_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29302.1"
                     /translation="MTDDALTAPAARRGLPGVPEDGLLASVLLSFLATAGLFYVNIMA
                     ALVDGLISGAGLTAKQAGLVASANVYGAAVGALAAVFVIRRLPWRPTAITVLLGLITI
                     DLASTLLRDPQVLIAVRFLHGLVGGLLVGVAFAVIARTKSPDRTFGMLLVVQFGLGGL
                     GLMFLPRLVPVFGPGVLFIALAAFSLATLAMTPFLGAYEAAPKVAASGPKAPTRWIPL
                     VLVFAAIFLFQAANMGLAAYIIGLGKGAGLELGFITDTLGAANWIGALGSVLVVVMGA
                     RFGRFWPIVAGLVLTLVGAWAFHFSELPWVYLVANIGTAITWAFVIPYLLGMSAAFDK
                     AGQMAALAGFFSKMGLASGPAMAAFVAAGGRYDGLINISVLGLALSGLAAIAPALALD
                     RQRPPG"
     gene            complement(2144..>3151)
                     /locus_tag="PLO65_13470"
     CDS             complement(2144..>3151)
                     /locus_tag="PLO65_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD29303.1"
                     /translation="DLDLAAFALAAPMDGDPPTAFGLVVDGVHYAGACQTRHGDYPFC
                     DGLDLPSYSTAKSIVGGIGLMRLEALHPGSARALIADHVPACARGDDWRGVTLADALD
                     MATGLYGSTAFEADENAPAGQVFFDVEDHAAKAAYACGHFPRRADPGTTFVYRTADTY
                     LLGSAMSDILRQAGEGDLYDDLVAPLWRSLRLSPTIMQTRRTYDAARQPFTGWGLTYH
                     RDDILRIAGWLEGGAVIDGRPALDGGLLTAALQKDPARPGLTAGGPAFRYKAGFWARD
                     IGGPLGCSRPVWAPFMSGFGGISVVLLPGGVTYYYFGDSGVFDWAPAAVAAGRIRDMC
                     S"
BASE COUNT          508 a         1082 c         1194 g          367 t
ORIGIN      
        1 gcccaggccg cccggctcgg ccgcgtccat gatcgccgcc cggccgatga cgcccgacag
       61 cgggaagccg ccggccagcg acttggccac cgtgaccagg tcgggctgca cgccgctgtg
      121 ctcgatgccg aacatcctgc cggtgcgggc gaagccggcc tgcacctcgt cggagatcag
      181 caggatgccg tgccggtcgc agatctcccg cagcgcgcgc agcagctccg gcggcgcggg
      241 ctggaagccg ccctccccct gcaccggctc gatgacgatg gccgccaccc gctcgggctc
      301 gacgtcggcg cggaacagga agtccagggc cctgaggctg tcctcgacgc tgaccccgta
      361 ctgcgggatc gggaacgggg cgtggaacac ctcggccggc agagggccga acagccgctt
      421 gtagggcgcg accttgccgg tcatggccat ggtcagcatg gttcggccgt ggaagccgcc
      481 ggtgaaggcg atgaccgccg accggcccgt cgcggcgcgg gcgatcttga ccgcgttctc
      541 gaccgcctcg gcgccggtgg tgaacaggat cgtcctggcc ggcccctcga acggcgccag
      601 ggcgttgagc cgctcggcca gggcgatgta gggctcatag gccaccacct ggaaggcggt
      661 gtgggtgtag gcgtcgagct gcgcgcggac cgccgccagc accttcggat ggcggtggcc
      721 ggtgttgagc acggcgatgc cgccggcgaa gtcgacgtag cgattgccct cgacgtccca
      781 cagctcggcg ttctcggcgc gggcggcgaa gatggcggtg gcggtggcga ccccgcgcgg
      841 cacggcggcc aggcggcggg tcaacaggtc agcgttggcg gacatgggca tgaactcctg
      901 gagacagact ggatgtttcg gccgccgcgg cggcaggggt gtggcgtggg tcagcccggt
      961 gggcgctgtc ggtcgagcgc gagggccggg gcgatcgcgg ccaggccgct gagcgccagg
     1021 ccgagaaccg agatgttgat caggccgtcg taccggccgc cggcggcgac gaaggcagcc
     1081 atggccgggc ccgaggccag ccccatcttg gagaagaagc cggccagcgc ggccatctgg
     1141 ccggccttgt cgaaggcggc gctcatgccg agcagatagg ggatgacgaa ggcccaggtg
     1201 atcgccgtgc cgatgttggc gaccagatag acccacggca gctcgctgaa atggaaggcc
     1261 caggcgccga cgagcgtcag gaccagtccg gcgacgatcg gccagaagcg gccgaaccgg
     1321 gcgcccatga cgaccaccag caccgagccg agcgcgccga tccagttggc cgcgccgagc
     1381 gtgtcggtga tgaagccgag ctccagtccg gcgcccttgc ccaggccgat gatgtaggcc
     1441 gccagtccca tgttggcggc ctggaacagg aagatggcgg cgaacaccag caccagcgga
     1501 atccagcggg tcggcgcctt tggaccgctc gccgcgactt tcggcgcggc ctcgtaggcg
     1561 ccgaggaagg gcgtcatggc cagggtcgcc aggctgaagg ccgccagggc gatgaacagc
     1621 acgccggggc cgaacaccgg gaccagccgc ggcaggaaca tcaggcccag gccgccgagg
     1681 ccgaactgca cgaccagcag catgccgaag gtgcggtccg gcgacttcgt ccgcgcgatg
     1741 accgcgaagg ccacgccgac cagcaggccg ccgaccaggc cgtgcaggaa ccgcaccgcg
     1801 atcagcacct gcggatcgcg cagcagcgtc gaggcaaggt cgatcgtgat caggcccagc
     1861 agcaccgtga tggcggtcgg ccgccagggc agccggcgga tgacgaacac cgccgccagg
     1921 gcgccgacgg ccgcgccata gacattggcc gaggccacca ggccggcctg cttggcggtc
     1981 aggccggcgc cgctgatcag gccgtcgacc agcgccgcca tgatgttgac gtagaacagg
     2041 ccggccgtgg ccaggaagga cagcaggacg ctggccagca ggccgtcctc cggaacccct
     2101 ggcaggccgc gacgcgcggc cggcgcggtg agggcgtcgt cggtcatgag cacatatccc
     2161 tgatccggcc cgccgcgaca gcggccgggg cccagtcgaa cacgccgctg tcgccgaaat
     2221 agtagtaggt cacgccgccc ggcagcagca cgacggagat gccgccgaag cccgacatga
     2281 agggcgccca gaccggccgg gagcagccca gcggcccgcc gatgtcgcgc gcccagaagc
     2341 cggccttgta acggaaggcc ggaccgcccg ccgtcaggcc gggacgggcg ggatccttct
     2401 gcagcgccgc cgtcaggagg cccccgtcga gcgccggacg gccgtcgatc acggcgccgc
     2461 cctcgagcca gccggcgata cgcaggatgt cgtcgcgatg gtaggtcagg ccccagccgg
     2521 tgaacggctg gcgggccgcg tcatacgtcc ggcgggtctg catgatcgtc gggctgagcc
     2581 gcaacgagcg ccacaggggc gcgaccaggt catcgtagag gtcgccctcg cccgcctgcc
     2641 ggaggatgtc ggacatcgcc gacccgagca ggtaggtatc ggccgtgcgg tagacgaagg
     2701 tggttccggg gtcggcgcgg cgtgggaagt ggccgcaggc gtaggcggcc ttggcggcat
     2761 ggtcctcgac gtcgaagaac acctggccgg ccggcgcgtt ctcgtccgcc tcgaaagccg
     2821 tcgaaccata gaggcccgtg gccatgtcca gggcgtcggc cagggtcacg ccgcgccagt
     2881 cgtcgccccg ggcgcaggcc ggcacatggt cggcgatcag cgcacgggcg ctgcccggat
     2941 gcagggcctc gagccgcatc aggccgatgc cgccgacgat cgacttggcg gtggaatagg
     3001 acggcaggtc gagcccgtcg cagaacggat agtcgccgtg ccgggtctgg caggcgccgg
     3061 cgtagtgcac gccgtcgacg accagcccga aggcggtcgg cgggtcgccg tccatcggcg
     3121 cggccagggc gaaggcggcg aggtccaggt c
//
LOCUS       DAORNX010000353         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23340_length_3141_cov_8.005833, whole genome shotgun sequence.
ACCESSION   DAORNX010000353 DAORNX010000000
VERSION     DAORNX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23340_length_3141_cov_8.005833"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLO65_13475"
     CDS             <1..569
                     /locus_tag="PLO65_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terpene utilization protein AtuA"
                     /protein_id="HRD29304.1"
                     /translation="SMSVGTTGWFGGAPTITPVARVFSILLPREQFPATVSVGDRTLT
                     VTPTAGGGHDPAKVVPPPRPADPAPDADMVTVPLVDLAWARSGDKGDAFNIGVIARDP
                     AWLPWIRAGLSEARMRSWFAHDFEGATDPKVIRYELPGMNALNLHFIQALGGGQFSSL
                     RLDPLAKGKAQQLLDIGIDIPRALAPGL"
     gene            668..1459
                     /locus_tag="PLO65_13480"
     CDS             668..1459
                     /locus_tag="PLO65_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29305.1"
                     /translation="MKLDSSVSAVVTGGASGLGAATVKALRDLGVKVAIFDLNPETGR
                     KKADELGALFCEVNVMDEASVDAGFAKARAAHGQERYLVNCAGGGRGGKTIARDKQTG
                     EIKPFVVSDFEWVLGLNTVGTFRCIVKSAAGMATLEPDEDGARGAIVNTGSVAAEDGQ
                     VGQVAYAASKAAIKGMTLVIARDLANEGIRINTILPGIMATPPMLGVKTRAPAIFDSL
                     STSVPFPRRLGHPEEYADLAVTMLRNGYFNGETVRLDGAIRMPPR"
     gene            complement(1490..2509)
                     /gene="ilvC"
                     /locus_tag="PLO65_13485"
     CDS             complement(1490..2509)
                     /gene="ilvC"
                     /locus_tag="PLO65_13485"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRD29306.1"
                     /translation="MRVYYDRDADLARILDRKIAIVGYGSQGHAHALNLRDSGLKNVA
                     VALRPGSASAKKAEGEGLKVMTVAEAAAWADGIMILAPDELQRGIYENEIAPNIRDGA
                     ALLFAHGLNVHFGLIEPKASVDVLMVAPKGPGHTVRGEYLKGGGVPCLIAIHQDATGN
                     AMDLGLAYASAIGGGRSGIIETNFREECETDLFGEQAVLCGGLVELIRAGFETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMSYSISNTAEYGAYVSGPRVVTPDTKAEMK
                     RILEDIQSGRFVRDFMLENQVGQPSFKATRRRAAEHPIEEVGGRLRAMMPWITKNKLV
                     DKARN"
     gene            complement(2701..3006)
                     /locus_tag="PLO65_13490"
     CDS             complement(2701..3006)
                     /locus_tag="PLO65_13490"
                     /inference="COORDINATES: protein motif:HMM:NF023204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRD29307.1"
                     /translation="MRNRDHGRRWYDSQSWRRAYHAQRRYRVAPYRYPSGWYARSWAY
                     NDFLPFGWFTASYYLNAGAYGLPYPPIGCEWVRVGQDALLVDIWTGRVISVYYGLFW"
BASE COUNT          485 a         1111 c         1066 g          479 t
ORIGIN      
        1 cctcgatgag cgtcggcacc accggctggt tcggcggcgc gccgaccatc acgcccgtgg
       61 cccgggtgtt ctcgatcctg ctgccgcgcg agcagtttcc ggcgaccgtg tcggtcggcg
      121 accggaccct gaccgtcacc ccgacggccg gcggcggcca tgatcccgcg aaggtcgtcc
      181 ccccgccgcg ccccgccgac cccgcgccgg atgccgacat ggtcaccgtg ccgctggtcg
      241 acctggcctg ggcccggtca ggcgacaagg gggacgcctt caacatcggg gtcatcgccc
      301 gcgacccggc ctggctgccg tggatccgcg cggggctgtc ggaggcccgg atgcggtcct
      361 ggttcgccca cgacttcgag ggcgcgaccg atccgaaggt catccgctat gagctgccgg
      421 gcatgaacgc cctgaacctg catttcatcc aggccctggg cggcgggcag ttctccagcc
      481 tgcggctcga cccgctggcc aagggcaagg cgcagcagct gctggacatc ggcatcgaca
      541 ttcccagggc cctcgcgccc ggtctttgaa tgcaggcgcg acgccgcttg cctgccccag
      601 cgtcgccggc gctcaactgc gccgacaagc ccggtcgcga aaggccggcg atcctgggag
      661 aacagccatg aaactcgaca gcagcgtttc cgccgtggtc accggcggcg cgtccggcct
      721 cggcgcggcg acggtcaagg ccctgcgcga cctgggtgtg aaggtcgcca tcttcgatct
      781 caaccccgag accgggcgga agaaggccga cgagctcggc gcgctgttct gcgaggtcaa
      841 tgtgatggac gaggcctcgg tcgacgccgg cttcgccaag gcccgcgcgg cgcacggcca
      901 ggagcgctac ctggtcaact gcgccggcgg cggccggggc ggcaagacca tcgcccgcga
      961 caaacagacc ggcgagatca agccgttcgt cgtgtccgat ttcgagtggg tgctgggcct
     1021 caacacggtc ggcacctttc gctgcatcgt caagagcgcg gccggcatgg ccacgctcga
     1081 gcccgacgag gacggcgccc gcggggccat cgtcaacacc ggctcggtgg cggccgagga
     1141 cggccaggtc ggccaggtcg cctacgcggc ctcgaaggcg gccatcaagg gcatgaccct
     1201 ggtcattgcc cgcgacctgg ccaacgaagg catccgcatc aacaccatcc tgcccggcat
     1261 catggccacg ccaccgatgc tgggcgtgaa gacccgggcg ccggcgatct tcgacagcct
     1321 gtcgacctcg gtgccgttcc cgcgccggct cggccacccg gaggaatacg ccgacctggc
     1381 cgtgaccatg ctgcgcaacg gctacttcaa cggcgagacc gtccggctgg atggcgcgat
     1441 ccgcatgccg ccccggtagc ggccgggcgg ctcgcgtccg cctgcggacc tagttccgcg
     1501 ccttgtcgac cagtttattc ttcgtaatcc acggcatcat cgcgcgcagg cggccaccga
     1561 cctcctcgat cgggtgttcg gcggcgcgcc gacgggtggc cttgaagctg ggctggccga
     1621 cctggttctc gagcatgaag tcgcgcacga aacggcccga ctggatatcc tcgagaatgc
     1681 gcttcatctc ggccttggtg tcgggcgtca ccacccgcgg cccgctgaca taggcgccgt
     1741 attcggcggt gttgctgatc gagtagctca tgttggcgat accgccttca tagatcaggt
     1801 cgacgatcag cttcacctcg tgcaggcatt cgaaataggc catctccggc gcgtagccgg
     1861 cctcgaccag ggtctcgaag ccggcccgga tgagctcgac caggccgccg cagagcacgg
     1921 cctgctcgcc gaacaggtcg gtctcgcatt cctcgcggaa gttggtctcg atgatgcccg
     1981 agcgaccgcc gccgatggcg ctggcgtagg ccaggccaag gtccatggcg ttgccggtgg
     2041 cgtcctggtg gatggcgatc aggcagggca cgccgccacc cttgaggtat tcgccacgca
     2101 ccgtgtggcc cgggcccttg ggcgcgacca tcagcacgtc gacgctggcc ttgggctcga
     2161 tcagcccgaa gtggacgttc aggccgtgag cgaacagcag ggccgcgccg tcacgaatgt
     2221 tcggcgcgat ctcgttctcg tagatgccgc gctgcagttc gtcgggcgcg aggatcatga
     2281 tgccgtcggc ccaggccgcc gcctcggcca ccgtcatgac cttcaggccc tcgccctcgg
     2341 ccttcttcgc cgaggccgag cccgggcgca gcgccacggc gacgttcttg aggccggaat
     2401 cgcggagatt gagcgcgtgg gcgtggccct ggctgccata gcctacgatc gcgatcttcc
     2461 ggtccagaat gcgggccaga tctgcgtcgc ggtcatagta gacgcgcatg atttttctcc
     2521 ggtttggtcg attgcgccgc acctgcgcaa cggcagtgca gcaaaggccg ggctatcgag
     2581 cggaaaattg cccgaaggtc aagggtcgga ccacgccccg cgacaatatt caactggccg
     2641 gcgcccaacg aaaacggccc cggagtcgcc tccggggccg tctcgatgcc gatgcgcgac
     2701 ctaccagaac aggccgtagt agacgctgat cacgcggccg gtccagatgt cgaccagcag
     2761 ggcgtcctgg ccgacgcgga cccactcgca gccgatcggc gggtacggca ggccataggc
     2821 tcccgcgttc agatagtagc tggcggtgaa ccagccgaac ggcaggaagt cgttgtaggc
     2881 ccagctgcgg gcgtaccagc cgctgggata gcggtagggg gccacgcgat agcggcgctg
     2941 ggcatggtag gcccgccgcc aggactgaga gtcataccag cgccggccgt ggtcgcgatt
     3001 gcgcatgccg cggccctcgc ccggacggcg tccatcccag cgctgtccgc gaccgtcgcg
     3061 atcccagccg ccgggcccgc cgggacgtcc gtcacgatcc caaccgccgg gacggccatc
     3121 cggccgaccg tcgcggtcgc g
//
LOCUS       DAORNX010000354         3133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23429_length_3133_cov_4.439896, whole genome shotgun sequence.
ACCESSION   DAORNX010000354 DAORNX010000000
VERSION     DAORNX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23429_length_3133_cov_4.439896"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..918)
                     /gene="lexA"
                     /locus_tag="PLO65_13495"
     CDS             complement(217..918)
                     /gene="lexA"
                     /locus_tag="PLO65_13495"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRD29308.1"
                     /translation="MLTRKQHELLMFIHERIKETGVSPSFDEMKEALDLASKSGIHRL
                     ITALEERGFLRRLPHRARALEVTKLPQQATTSAPSGGRQAFKPQVVPGDRLTTPPPSA
                     IANDVREIPLLGRIAAGSPIEALQVERDRMLVSESMLGAGEHYVLEVQGDSMIEAGIL
                     DGDFVLIRKTDTAHSGEIVVALVQGEEATLKRLRRKGASIALEAANPKYQTRVYGPDQ
                     VEVQGKLVGLIRKYH"
     gene            1122..1340
                     /locus_tag="PLO65_13500"
     CDS             1122..1340
                     /locus_tag="PLO65_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29309.1"
                     /translation="MTVEGKGPGQAIAVIDYGPEHHLIWVVALDDSGEIWSAPNPKVR
                     LQSNWTMGRKARAAGEVESLRPAAVRAG"
     gene            complement(1327..>1606)
                     /locus_tag="PLO65_13505"
     CDS             complement(1327..>1606)
                     /locus_tag="PLO65_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRD29310.1"
                     /translation="EMDRAGAMGATLVGVNNRDLRSFTVDLSITERLAARAPAGSLLV
                     TESGLFTAADAARLEACGAKAMLVGESLMRQANVEAATRALLGAATPP"
     assembly_gap    1607..1658
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1659..2126)
                     /locus_tag="PLO65_13510"
     CDS             complement(<1659..2126)
                     /locus_tag="PLO65_13510"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRD29311.1"
                     /translation="MTDILTRIAAYKRDEVAGRKSARSRADIEAAAMAAGAPRGFKAA
                     LERHHAPGRLALIAEIKKASPSKGLIRGDFDPPALARAYEAGGAACLSVLTDGPSFQG
                     ADSYLVAARAATALPCIRKDFLVDPWQVAESRALGADAILVILAMIDDALAADL"
     gene            complement(2226..>3133)
                     /gene="trpD"
                     /locus_tag="PLO65_13515"
     CDS             complement(2226..>3133)
                     /gene="trpD"
                     /locus_tag="PLO65_13515"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRD29312.1"
                     /translation="PAQVAAAVTAIRLRGETVGEIVACARAMRRAAVHLDLPYETVDV
                     CGTGGDGLHTLNISTAVGFVAAGGGLKVAKHGNRAITSKSGTADVLAALGVNIDPGLD
                     RQRAALDQAGICFLFAQAHHGAMKHVSPIRQQLGFRTIFNLLGPLTNPAGARRQVVGV
                     SAPRFVEPIAQALGALGAVRAWSVHGQGMDELTTTGETEVAEWRDGGVRLFQITPEAV
                     GLARSSLADLTGGDPDFNATALRDLLKGKTGAYRDIVLLNAAAAFLVADRVETLREGV
                     ERAGAVIDDGRALKALDQLVAITNG"
BASE COUNT          462 a         1052 c         1090 g          477 t
ORIGIN      
        1 cgcgggacgg ctggcgggtg cggtgggcga atgacgcgcg ggggcggcgg ccgtggacgg
       61 ggtaggccgt cacggttcct gccgtcatcg tcggacttgt tccgaccacc caggaccatc
      121 ggcgtcaagg aaacgggggc gcgacccaag ctgcgccccc ttctgaagct tcgtgtgcat
      181 ggagcctcgc gacaagcgcg aggatgacgg ccggagtcag tgatacttgc ggatcaaccc
      241 gaccagcttg ccctggacct cgacctggtc aggaccatag acgcgggtct ggtacttcgg
      301 gttggccgcc tcgagcgcga tgctggcgcc cttgcggcgc aggcgcttga gggtggcttc
      361 ctcgccctgg accagggcca cgacgatctc gccgctgtgg gcggtgtcgg tcttgcggat
      421 cagcacgaag tcgccgtcga ggatgccggc ttcgatcatc gagtcgccct gcacctcgag
      481 cacatagtgt tcgcccgcgc cgagcatgct ctcggacacc agcatcctgt cgcgctcgac
      541 ctgcagcgcc tcgatcggcg agccggcggc gatccggccc agcagcggaa tctcgcgcac
      601 gtcattggcg atggctgacg ggggcggcgt ggtcagtctg tcgccgggca cgacctgcgg
      661 cttgaacgcc tgtcgcccgc cggacggcgc ggatgtcgtc gcctgctgcg gcagcttggt
      721 cacctcgagc gcccgggccc ggtgcggcag gcggcgcagg aaaccgcgtt cctcaagcgc
      781 cgtgatcagc cggtggatgc cggacttgga ggccaggtcg agcgcctcct tcatctcgtc
      841 gaacgacggg gagacgccgg tctccttgat ccgctcgtgg atgaacatga gcagttcgtg
      901 ctgcttgcgg gtgagcatct gccgggcctc gccaccgaat ctgtcgcgga acaaaccgct
      961 aacaactgtc gatgttctct aggtgttctt cgtttctgtc aagttaacgc cgtttcgcgg
     1021 ccgaagaagc caaggaacag cgtggcttgc cggccgtttt cgccgggcgg acccccagtg
     1081 tgagacccat ggcgttcact caattgcaga caccgattcc catgaccgtc gagggaaagg
     1141 gccctggcca ggccatcgcg gtcatcgact atggccccga acaccacctg atctgggtcg
     1201 ttgcgctcga cgacagcggc gagatctgga gcgcgcccaa tccgaaggtg cggctgcaat
     1261 cgaactggac catgggccgc aaggctcggg cggccggcga ggtcgaatcg ctgcggccgg
     1321 cggcggtcag ggcggggtag ccgcgcccag cagcgcccgg gtcgccgcct cgacgttggc
     1381 ctgacgcatc aggctttccc cgaccagcat agccttcgcc ccgcaggctt ccagccgggc
     1441 cgcgtcggcg gcggtgaaga ggccgctctc ggtgaccagc agcgatccgg ccggcgcgcg
     1501 cgcggcgagc cgctcggtaa tcgacaggtc gacggtgaag ctgcgcaggt cgcgattgtt
     1561 gaccccgacc agcgtcgcac ccatcgcgcc ggcgcggtcc atctcgnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ggtcagcggc cagggcgtcg
     1681 tcgatcatcg ccaggatgac caggatggcg tccgcgccca gggcgcggct ctcggccacc
     1741 tgccagggat cgacgaggaa gtccttgcgg atgcagggca gggcggtcgc cgcccgggcg
     1801 gcgaccagat agctgtcggc gccctggaag ctcggcccgt cggtcagcac cgacaggcag
     1861 gccgcgccgc cggcctcata ggcgcgggcc agcgccggcg gatcgaagtc gccgcgaatc
     1921 aggcccttgg atggcgaagc cttcttgatc tcggcgatca gggccaggcg gcccggggca
     1981 tggtggcgct cgagggccgc cttgaaaccc cgcggggcgc cggcggccat cgccgcggcc
     2041 tcgatgtccg cccgtgagcg cgcggacttc cgcccggcca cctcatcccg cttgtaggcg
     2101 gcgattctgg tgaggatgtc ggtcatgact ccacacccga ttgctcccgc gtaggcggga
     2161 gcccaagcag gggtagccga caaatcgctt gggtccccgc ctgcgcgggg acaatcggat
     2221 tgaagctagc cattggtgat cgccaccagc tggtccagcg ccttcagcgc ccgcccgtcg
     2281 tcgatcaccg ctccggcccg ttcgaccccc tcgcgcaggg tctcgacccg gtcggcgacg
     2341 aggaaggcgg cggcggcgtt gagcaggacg atgtcgcgat aggcgccggt cttgcccttc
     2401 agcagatcgc gcagggcggt ggcgttgaag tcaggatctc cgcctgtcag gtcggcaagc
     2461 gaacttcgag ccagtccaac ggcttccggc gtaatctgaa acagacgaac gccaccgtcg
     2521 cgccattcgg cgacctcggt ttcgccggtc gtggtcagct cgtccatgcc ctggccatgc
     2581 accgaccagg cccgcaccgc gcccagggcg cccagggcct gggcgatcgg ctcgacgaag
     2641 cgcggcgccg agaccccgac cacctgccgg cgcgcgccgg ctggattggt caacggcccc
     2701 aacagattga aaatcgtgcg gaatccgagc tgctggcgga tcggcgagac atgtttcatc
     2761 gccccgtggt gggcctgggc gaacaggaaa cagatgccgg cctggtcgag cgccgcgcgc
     2821 tgcctgtcga ggcccggatc gatgttgacc cccagggcgg ccagcacatc ggccgtgccg
     2881 gacttgctgg tgatggcccg gttgccgtgc ttggccacct tcagcccgcc gccggccgcc
     2941 acgaagccga cggccgtgga gatgttcagc gtatgcaggc cgtcgccgcc cgtgccgcag
     3001 acgtcgaccg tctcgtaggg caggtcgagg tgcacggcgg cccggcgcat ggcgcgggcg
     3061 caggcgacga tctcgcccac agtctcgccg cgcaggcgga tggcggtgac ggcggccgcc
     3121 acctgggccg ggg
//
LOCUS       DAORNX010000355         3126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23491_length_3126_cov_7.373494, whole genome shotgun sequence.
ACCESSION   DAORNX010000355 DAORNX010000000
VERSION     DAORNX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23491_length_3126_cov_7.373494"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1645)
                     /locus_tag="PLO65_13520"
     CDS             complement(65..1645)
                     /locus_tag="PLO65_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HRD29313.1"
                     /translation="MASVTDTASPEPEASARRRLWPQGSRLGRLIIVLNFLGLAILVI
                     GALTLNEMRRGLVNARIESLITQGEWVARLIDEAATVGEPEPALQPQRAAQLLQLLHL
                     PRSQRVRLFDKDGRLIADSYFVADRVDWKPLPPARKPGQKAPAPERNPTEERAARALK
                     AELAAAMRGEQVKGRRIGEGGEQLVSVSLPIQRVRAVLGVLTLEAGDVDSIIAAERAA
                     LIPFILIALGVTTLSSFLIYALVARPVLRLARAADRVQQARARSISMPDLAGRQDEIG
                     DLTRSLEQMTDSLSERMDAIERFAADVAHEIRNPLTSIRSAVETLDLVGEGPARARLT
                     GILKQDVGRLDRLVTDIANASRLDAELSRDEPRRIDLVRLIGDIVHVYGASADPGAVT
                     VRILAAEPAEALVVAGREGPLGQVFRNLIDNARSFSPPGGEVRVTLSRTRGRVAVAVE
                     DDGPGIPPDNLETVFERFYTSRPKGTAFGGNSGLGLSIARQIVAAHGGDIHAENRTDA
                     SGAIVGARFVVHLPERAG"
     gene            complement(1614..2321)
                     /locus_tag="PLO65_13525"
     CDS             complement(1614..2321)
                     /locus_tag="PLO65_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD29314.1"
                     /translation="MANITLVDDDENIVASVSLALESHGHTVTACHDGVAGLESLQNN
                     PPDLAILDVKMPRMDGMELLRRLRQTSDLPVIMLTSKDEEIDEILGFNLGADDYIHKP
                     FSQRLLLERVKAVLRRAGLDEAIKAGGPVDPGARSMKRGKLTLDPERHDSLWDGKPVR
                     LTVTEFLLLQSLAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHIKRMRKKFRQVDPT
                     FDAIETLYGVGYRYRES"
     gene            2468..>3126
                     /locus_tag="PLO65_13530"
     CDS             2468..>3126
                     /locus_tag="PLO65_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29315.1"
                     /translation="MERGQVHYELFVRRQPGSGWSLDMATEDRARAIETAEQALSDGR
                     AIAVRVTKETLDPESREFRSVTILSKGAPERTRTRKVRENTEPLCVSPADLYTCHARE
                     RIGRLLDNWLARNKATPFELLHRPDLVEKLEASGVELQHAIQKVAVPEAQARGMGVHE
                     IIRSFQSLAERAIERLMKDHRKGVLPNLDREGFAAAAERLVGEPDRAYLLGAGVAAAI
                     AP"
BASE COUNT          476 a         1108 c         1083 g          459 t
ORIGIN      
        1 ccctcgatca gcacaccgcg ccagacgccg cgccggcgca gcgcgaccag gccggcgtgg
       61 cggatcaccc ggcccgctcc ggcagatgca cgacgaagcg cgcgccgacg atcgccccgc
      121 tcgcgtcggt gcggttctcg gcatggatgt cgccgccgtg cgccgcgacg atctggcggg
      181 cgatcgacag gccgagaccg gagttgccgc cgaacgccgt gcccttgggc cgcgaggtat
      241 agaaccgctc gaacacggtc tcgaggttgt cgggcgggat gcccgggccg tcgtcctcga
      301 ccgccacggc gacccgcccc cgtgtccgtg acagggtcac ccgcacctcg ccgccgggcg
      361 ggctgaacga ccgggcgttg tcgatcaggt tccggaaaac ctggcccagc ggcccttcgc
      421 ggccggccac gaccagggct tcggcgggtt cggccgccag gatccgcacc gtcaccgccc
      481 ccgggtcggc cgaggcgccg tagacgtgga cgatatcgcc gatcaaccgc accagatcga
      541 tgcggcgggg ctcgtcgcgc gacagctccg cgtccagtcg cgaggcgttg gcgatgtcgg
      601 tgaccagccg gtcgagccgg cccacatcct gcttgaggat cccggtcagc cgcgcgcgcg
      661 ccggcccctc gccgaccagg tcgagcgtct cgaccgccga gcggatcgag gtcaggggat
      721 tgcggatctc gtgggccacg tcggcggcga accgctcgat ggcgtccatc cgctcagaca
      781 ggctgtcggt catctgctcg agggagcggg tcaggtcgcc gatctcgtcc tgccggccgg
      841 ccagatccgg catggagatg gaccgcgccc gggcctgctg cacgcggtcg gcggcccggg
      901 ccaggcgcag caccggccgc gccaccaggg cgtagatcag gaaactcgac agggtggtga
      961 cgccgagcgc gatgagaatg aacgggatca gcgcggcccg ctcggcggcg atgatcgaat
     1021 cgacgtcgcc ggcctccagg gtcagcacgc ccagcacggc ccgcacccgc tggatcggca
     1081 gggataccga caccagctgc tcgccgccct cgccgatgcg ccgccccttg acctgctcgc
     1141 cgcgcatggc cgcggccagc tccgccttga gggcccgggc ggctcgctcc tcggtcggat
     1201 tgcgctcggg ggcgggagcc ttctgacccg gcttgcgggc cggcggcaac ggcttccagt
     1261 cgacccggtc ggcgacgaaa taggagtcgg cgatcaaccg gccgtccttg tcgaacagcc
     1321 gcacgcgctg gctgcgcggc aggtgcagca gttgcagcag ctgggcggcc cgttgcggct
     1381 gcagcgccgg ctcgggctcg ccgaccgtgg ccgcctcgtc gatcagccgg gcgacccact
     1441 cgccctgggt gatcaggctt tcaatgcgcg cgttgaccag gccgcggcgc atctcgttga
     1501 gcgtcagggc tccaatcacc aggatcgcca gacccaggaa attgaggacg atgatcagcc
     1561 ggccgaggcg cgagccctgg ggccacagac ggcggcgcgc ggacgcctcc ggctcaggac
     1621 tcgcggtatc ggtaaccgac gccatacagg gtctcgatcg cgtcgaaggt cggatccacc
     1681 tggcggaact tcttgcgcat ccgcttgatg tggctgtcga tcgtgcggtc gtcgacatag
     1741 acctgatcgt cgtaggccgc gtccatcagg ttgtcgcggc tcttcacgaa cccgggccgc
     1801 tgggccagcg actgcagcag caggaactcg gtcacggtca accgcaccgg cttgccgtcc
     1861 cacaggctgt catgccgctc gggatcgagg gtcagcttgc cgcgcttcat cgagcgggcg
     1921 ccgggatcga ccggaccgcc ggccttgatc gcctcgtcca ggcccgcgcg gcgcagcacc
     1981 gccttgaccc gctccagcag cagccgctgg ctgaacggct tgtggatata gtcgtcggcg
     2041 ccgaggttga agccgaggat ctcgtcgatc tcctcgtcct tgctggtcag catgatcacc
     2101 ggcaggtcgg acgtctggcg caggcggcgc aacagctcca tcccgtccat gcgcggcatc
     2161 ttcacgtcga ggatggccag gtccggcgga ttgttttgca gggactcgag gccagcgacc
     2221 ccgtcgtgac aggccgtcac ggtgtgacca tggctctcca gcgccagtga aacggaggcg
     2281 acgatgttct cgtcgtcgtc caccagggtg atgttggcca ttgcagtcct tcgtttgagc
     2341 gtctcgcggg ctgaaaccta gcgcggtccc ggcccggcgc aaggaaatca cccgccagca
     2401 tggccccatt gcggcgcttt cgcatcgtaa acacgacctt agggccttgc ccttatggat
     2461 gcgggtcatg gaacggggtc aggttcacta cgaactgttc gtgcgtcgcc agcccggctc
     2521 gggctggtcg ctcgacatgg cgaccgagga tcgcgcccgg gcgatcgaga ccgccgagca
     2581 ggcgctgagc gacggccgcg ccatcgccgt gcgcgtgacc aaggaaaccc tcgacccgga
     2641 gagccgggaa ttccgttcgg tgaccatcct gtccaagggc gcgcccgaac ggaccaggac
     2701 gcggaaggtc cgcgagaaca ccgagccgct gtgcgtcagc ccggcggacc tctacacctg
     2761 tcacgcgcgc gagcggatcg gccggctgct cgacaactgg ctggcgcgca acaaggccac
     2821 ccccttcgaa ctgttgcacc ggcccgacct ggtcgaaaag ctcgaagcct ccggcgtcga
     2881 gctgcagcat gccatccaga aggtcgccgt gcccgaggcc caggcccgcg gcatgggggt
     2941 gcacgagatc atccggtcgt tccagtcgtt ggccgaacgg gccatcgagc ggctgatgaa
     3001 ggaccatcgc aagggcgtgc tgcccaacct tgaccgcgaa ggcttcgcgg ccgccgccga
     3061 acggctggtc ggcgagcccg accgcgccta cctgctgggc gcgggcgtcg ccgcggcgat
     3121 cgcacc
//
LOCUS       DAORNX010000356         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23593_length_3117_cov_7.050621, whole genome shotgun sequence.
ACCESSION   DAORNX010000356 DAORNX010000000
VERSION     DAORNX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23593_length_3117_cov_7.050621"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="PLO65_13535"
     CDS             <1..1518
                     /locus_tag="PLO65_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD29316.1"
                     /translation="ARARMEAAGVLLETLGPGAIGLDCSRRLTDIRQRLPGYANLIRF
                     DRIGRVACSAESVPADPQRRDRPWFRQLAAGEALVMTLAPGAAYAAEPSLFTAARSYD
                     PQSGFNVLAAVIPLSSLRPELEDRSLPRGSQVAVVDRDGRFLSVTDEMAFPRTVKSWI
                     DGAGARGSYLGYGLDRHGVRRVYSAAPLVGDNLFVILSAPSAGLFSTAWLDPLSGIVF
                     PLIAFVVALIAVVFATERGVGRWILYLQRIAAIYARGRFTVRPLQAERAPPEIRELAE
                     TLDHMAATIVARDATLHENLDQKDALMREIHHRVKNNLQVISSLLSMQERALSDPAAR
                     MAMSDTRQRITALALIYRALYQGPDIKHVDLKPFLEELIGQLLAGDIGGGGHIRTEVR
                     ADRLSIDPDKLAPLALFAVEAVTNAQKHALQGRGGYLGVDFTLRDGEAELAIADDGGG
                     QTAAPDAATAGGVGRTLMTAFARQLRGRAALEANSLGGVTARLVFPMPETRAAGT"
     gene            1589..1723
                     /locus_tag="PLO65_13540"
     CDS             1589..1723
                     /locus_tag="PLO65_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HRD29317.1"
                     /translation="MRKIVILAVAAASLFAAACNTIEGAGKDVQAAGQAVEDTANDAK
                     "
     gene            complement(1792..3081)
                     /locus_tag="PLO65_13545"
     CDS             complement(1792..3081)
                     /locus_tag="PLO65_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD29318.1"
                     /translation="MSGAERIPDEIAFQAVNPAAYADDSVHEAFRWLRANNPLGKAEL
                     EGVYPFWLVTRHADILEISRQNDLFHSGDMPTTFTTIEGDRMVRAMTGGSPHLVRTLV
                     QMDAPDHPKYRIMTQSWFLPQNIRKLEDRIRQIARQHVDHMASLGGECDFVNEVALFY
                     PLRVIMEILGVPPEDEPRMLKLTQELFGAQDPDLNRNQSEAGAMQGPEALAAIQSVIA
                     DFFMYFKAITDDRKASPRDDVASVIANGKLDGEPINDFEAMSYYVIVATAGHDTTSSS
                     TAGAIWGLCQYPEELAKLRADPSLIPGLVDEAIRWTTPVKHFMRSATADTEIGGRKIA
                     KGDWLWLAYPSGNRDETVFDEPDAFRVDRTGGKHLAFGYGAHVCLGQHLAKMEMRILF
                     EELIPRLTSLEFAGEPRRSVASFVGGPKVLPIRYGLT"
BASE COUNT          448 a         1116 c         1056 g          497 t
ORIGIN      
        1 gcccgcgcgc ggatggaagc cgccggcgtc ctgctcgaga ccctcgggcc cggcgcgatc
       61 ggcctcgact gctcccgacg cctgaccgac atccgccagc gcctgcccgg ctacgccaat
      121 ctgatccgct tcgaccgcat cgggcgggtg gcctgctcgg ccgagagcgt gcccgccgac
      181 ccgcagcgac gcgaccgtcc ctggttccgc cagctggccg ccggggaagc gctggtgatg
      241 acgctcgccc ccggcgcggc ctacgccgcc gaaccaagcc tgttcacggc cgcccggtcg
      301 tatgatcccc agtcggggtt caatgtgctg gccgcggtca ttcccctgtc cagcctcagg
      361 ccggagctgg aggaccgctc cctgccccgt ggctcgcagg tggcggtcgt cgatcgcgac
      421 gggcgtttcc tgtcggtgac cgacgaaatg gcctttccgc ggacggtgaa gagctggatc
      481 gacggggccg gcgcgcgcgg ctcctacctc ggctacggtc tggaccggca cggcgtccga
      541 cgggtctatt cggccgcccc gctggtcggc gacaatctgt tcgtcatcct gtcggccccg
      601 tcggccggac tgttctcgac ggcctggctg gacccgctgt cgggcatcgt cttcccgctg
      661 atcgccttcg tcgtcgccct gatcgcggtg gtcttcgcga ccgagcgcgg ggtgggccgg
      721 tggattctct atctgcagcg catcgccgcc atttacgccc ggggccggtt caccgtccgg
      781 ccgcttcagg ccgagcgcgc gccgcccgaa atccgcgagc tggccgaaac cctcgaccac
      841 atggccgcca cgatcgtcgc ccgcgacgcg acactgcacg aaaacctgga ccagaaggac
      901 gcgctgatgc gcgagatcca tcaccgggtg aagaacaacc tgcaggtcat cagctccctg
      961 ctgagcatgc aggaacgcgc gctcagcgat ccggctgcgc ggatggcaat gagcgacacg
     1021 cgccagcgga tcaccgccct ggccctgatc tatcgcgccc tctaccaggg gccggacatc
     1081 aagcacgtcg acctcaagcc cttcctcgag gaactgatcg gccagctgct ggccggcgac
     1141 atcggcggcg gtgggcacat ccgcaccgag gtgcgggccg accggctgtc catcgatccc
     1201 gacaagctgg ctccgctggc cctgttcgcc gtcgaggccg tgaccaacgc ccagaagcat
     1261 gcgttgcagg gccggggcgg gtacctgggc gtcgacttca ccctgcgcga cggcgaggcc
     1321 gaactggcga tcgccgatga cggcggcggc cagaccgccg cgcccgacgc cgccaccgcc
     1381 ggcggagtcg gccgcacgct gatgaccgcc ttcgcccgcc agttgcgggg tcgggcggcc
     1441 ctggaagcca acagcctggg cggcgtcacc gcccgcctgg tctttccgat gccggagacc
     1501 cgcgccgccg gaacctgacc gcctcacgag gcgttgatcg ggcgtgccac aaccgccccg
     1561 cctcgccggg gcccgaggag aacgcgacat gcgcaagatc gttattctgg ccgttgccgc
     1621 cgccagcctg ttcgccgcgg cctgcaacac catcgagggc gcgggcaagg acgtccaggc
     1681 cgccggccag gccgtcgagg acaccgccaa cgacgccaag taggctcacg ccgcaacccc
     1741 gacgacaacg gccctcgccc tcgcgggcgg gggccgtttc gcgtctggtg atcaggtcag
     1801 gccgtagcgg atcggcagaa ccttcgggcc cccgacgaag ctggccacgc tgcggcgcgg
     1861 ctcgccggcg aactccagcg aggtcagccg cggaatcaac tcctcgaaca ggatgcgcat
     1921 ctccatcttg gccaggtgct ggcccaggca gacatgggcg ccgtagccga aggccaggtg
     1981 cttgccgccc gtccggtcga cccggaaggc gtccggctcg tcgaacaccg tctcgtcgcg
     2041 attgccggag ggataggcca gccacagcca gtcgcccttg gcgatcttgc ggccgccgat
     2101 ctcggtgtcg gccgtcgccg atcgcatgaa gtgcttcacc ggggtggtcc agcggatggc
     2161 ctcgtcgacc aggcccggga tcagcgacgg atcagccctc agcttggcca gttcctccgg
     2221 atactggcac aggccccaga tggcccccgc cgtcgatgac gaggtggtgt cgtggccggc
     2281 ggtggcgacg atgacgtagt agctcatcgc ctcgaagtcg ttgatcggct cgccgtcgag
     2341 ctttccgttg gcgatcacgc tggccacgtc gtcgcgcgga ctggccttgc ggtcgtcggt
     2401 gatcgccttg aaatacatga agaagtcggc gatcacgctc tggatcgcgg cgagggcctc
     2461 cggcccctgc atggcgccgg cctcgctctg gttgcggttc aggtccggat cctgggcccc
     2521 gaacagctcc tgggtcagct tgagcatccg cggctcgtcc tcgggcggca cgccgaggat
     2581 ctccatgatc acccgcagcg ggtagaacag cgccacctcg ttgacgaagt cgcactcgcc
     2641 gccgagcgag gccatgtggt cgacgtgctg gcgcgcgatc tggcggatgc ggtcctccag
     2701 cttgcggatg ttctgcggca ggaaccacga ctgggtcatg atccggtact tggggtggtc
     2761 gggcgcgtcc atctgcacca gcgtccgcac caggtgcggg ctgccgccgg tcatggcgcg
     2821 caccatgcgg tcgccctcga tggtggtgaa ggtcgtcggc atgtcgcccg aatggaacag
     2881 gtcgttctgc cggctgatct cgaggatgtc ggcgtgtctg gtgaccagcc agaacggata
     2941 gaccccctcc agctcggcct tgcccagcgg gttgttggcg cgcagccagc ggaaggcctc
     3001 gtgcacgctg tcgtcggcat aggccgccgg gttcaccgcc tggaaggcga tctcgtctgg
     3061 aatgcgttct gcgccgctca tggtcgtttc ccgttatgcc ccgcgcgaag gatgccg
//
LOCUS       DAORNX010000357         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23648_length_3111_cov_9.629908, whole genome shotgun sequence.
ACCESSION   DAORNX010000357 DAORNX010000000
VERSION     DAORNX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23648_length_3111_cov_9.629908"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLO65_13550"
     CDS             complement(<1..1076)
                     /locus_tag="PLO65_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29319.1"
                     /translation="MDFQLAEEHQMLKDLVAKFVRDQLIPLEPAVLARDAAGESIALT
                     AAERAPIDKVSKELGLWGLDAPEDVGGADLPMVAMVGVYEEMGKTITPYVLPPDSPNL
                     RMLMATVNEEQRVQYLAPYVAGEAISAIAISEPGAGADPAGMITRAVRDGDDWVINGR
                     KIWISKAKEADFTVTMAVTDPAKGARGGISAFLVDKGTPGFNVLRAIPMIGGQVTYEV
                     VYEDCRIPGSKLLGEEGQGFAPMQTRLSTRRLQMGIWGYTLAQRALDMMIEHAGQRET
                     FGAKLADRQAIQWWVADASTKIHAARLMAYDIAWKLDQGRDVRVEISMLKVFGTELGW
                     EVIDQAMQAFGAMGMTKELPLQMMA"
     gene            complement(1216..2070)
                     /locus_tag="PLO65_13555"
     CDS             complement(1216..2070)
                     /locus_tag="PLO65_13555"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD29320.1"
                     /translation="MVTVKPNPSGFGAEITGLDISAPLPADQLAAVNAAWAAHGVVWF
                     PDQPLDHDQLEAFTLQFGDFGWDPYVKPLADRPHILEVRREPDETASVFGGAWHSDWS
                     FQATPPAATILHSKVVPPVGGDTEFADGCRAYEALSPVMQGILDGLTTIHSATRPYGA
                     NGIYANEAPGRSMEIINCVEADNQQTHPLVRVHPVSGRKALFVNPIYTLGVAGMTEAE
                     SDNLLTFLYRHMLRQEFVYRHKWRPDMLILWDNRCCLHNATGGYDGHRRVMHRTTVAG
                     EAPIPVAQ"
     gene            complement(2255..3085)
                     /locus_tag="PLO65_13560"
     CDS             complement(2255..3085)
                     /locus_tag="PLO65_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD29321.1"
                     /translation="MSVTITMASEPYEHLELRQEGDALWVTMNRPDSLNALNRRLTAE
                     LRDLFVNLYWRRDVRVVVLSGAGPAFCAGLDLKERSDSNGQRRIADGLDSQRSIAEIV
                     IAMRRCPQPIIACVDGAAAGGGFALALASDVRIVTPKTRMNAAFIRIGLSACDIGVSY
                     FLPRMVGSSVAAEFMLTGRFIGADRAYQLGLASCVVAPDQVETEARGFVKDMLHATPL
                     GLRLTKEALNHAIDANGLEAAIAMEDRNQILASSDGDFAEGVRAFLEKREPDYSKTRG
                     "
BASE COUNT          472 a         1052 c         1066 g          521 t
ORIGIN      
        1 gccatcatct gcagcggcag ctccttggtc atgcccatgg cgccgaaggc ctgcatggcc
       61 tggtcgatga cctcccagcc cagctcggtg ccgaacacct tgagcatcga gatctcgacc
      121 cgcacgtcgc ggccctggtc gagtttccag gcgatgtcgt aggccatcag ccgggcggcg
      181 tggattttcg tggaggcgtc ggcgacccac cactggatcg cctggcggtc ggccagcttc
      241 gccccgaagg tctcccgctg gccggcgtgc tcgatcatca tgtccagcgc gcgctgggcc
      301 agggtgtagc cccagatgcc catctgcagg cggcgggtcg aaaggcgggt ctgcatcggg
      361 gcgaacccct gcccctcctc gccgagcagc ttcgagccag ggatgcggca gtcctcgtag
      421 accacctcat aggtcacctg gccgccgatc atcgggatgg cgcgcagcac gttgaagccg
      481 ggcgtgccct tgtcgaccag gaaggccgag atgccgccgc gcgcgccctt cgccgggtcg
      541 gtgaccgcca tggtcacggt gaagtccgct tccttggcct tgctgatcca gatcttgcgg
      601 ccgttgatca cccagtcgtc gccgtcgcgg accgccctgg tgatcatccc ggccgggtcg
      661 gcgcccgcgc ccggttcgga aatggcgatc gccgagatgg cctcgccggc gacgtagggc
      721 gccagatact ggacccgctg ctcctcgttc accgtggcca tcagcatgcg caggttcggc
      781 gagtccggcg gcaggacgta gggcgtgatg gtcttgccca tctcctcgta gacaccgacc
      841 atggcgacca tcggcaggtc ggcgccgccg acgtcctcgg gcgcgtccag gccccagagg
      901 cccagctcct tgctgacctt gtcgatcggc gcccgctcgg cggcggtcag ggcgatcgac
      961 tcgccggcgg cgtcgcgggc cagcacggcc ggctcgagag ggatcagctg gtcgcggacg
     1021 aacttcgcga ccagatcctt gagcatctgg tgctcttcgg cgagttggaa atccaagtca
     1081 gatcctcttc aaatgctcac attgtcatcc cggccgaagc gcagcggaga gccgggaccc
     1141 agatcaaatc ccgtcctcgg tgcgtgaagc cgaatctggg tcccggatcg ccttcggcgt
     1201 ccgggatgac aagatttatt gcgcgacggg tatcggagcc tcccccgcca ccgtggtccg
     1261 gtgcatgacc cggcgatggc cgtcatagcc gcccgtcgcg ttgtgcagac agcagcggtt
     1321 gtcccacagg atcagcatgt cgggccgcca tttgtggcga tagacaaact cctgccgcag
     1381 catgtggcgg tagaggaagg tcagcagatt gtccgattcc gcctcggtca tcccggcgac
     1441 gccaagggtg tagatcggat tgacgaacag cgccttgcgc ccgctgaccg ggtggacgcg
     1501 caccagcgga tgggtctgct ggttgtcggc ctcgacgcag ttgatgatct ccatcgaccg
     1561 gcccggcgcc tcgttggcgt agatgccgtt ggcgccgtag ggccgggtgg ccgaatggat
     1621 cgtcgtcagg ccatcgagga tgccctgcat caccgggctc agcgcctcgt aggcgcggca
     1681 gccgtcggcg aactcggtgt caccgccaac gggcgggacg accttcgaat gcaggatcgt
     1741 cgcggcgggc ggcgtcgcct ggaagctcca gtcgctgtgc caggcgccgc caaagacgct
     1801 ggcggtctcg tcgggctcgc ggcgcacctc gaggatgtgc ggccggtcgg ccaggggctt
     1861 cacataggga tcccagccga agtcgccgaa ctgcagggtg aaggcctcca gctggtcatg
     1921 gtcgagcggc tggtcgggaa accagaccac cccgtgcgcc gcccaggccg cattgaccgc
     1981 cgccagctgg tcggccggca gcggggcgct gatgtcgagc ccggtgatct cggcgccgaa
     2041 accggaggga ttgggtttca ctgtgaccac gatgcgcctc cccttttcct tcacagtcgg
     2101 cctgaatgac ggccgccgca atcatccagc gtggttcgac ccccggatca aatccggggg
     2161 ccttcggcgc tgctcaccat gacgtggcga gggagcccgt cctgcacgtc atcctgagca
     2221 ggcccgcagg gccgtgtcga aggacgcacg caccctaccc ccgcgtcttc gaatagtccg
     2281 gctcgcgctt ttccaggaag gcccgcaccc cctcggcgaa gtcgccgtcg ctgctggcca
     2341 ggatctggtt gcggtcttcc atggcgatgg ccgcctcgag gccgttggcg tcgatggcgt
     2401 ggttcagcgc ttccttggtc aggcgcaggc cgaggggcgt cgcatgcagc atgtccttga
     2461 cgaacccgcg ggcctcggtc tcgacctggt cgggcgcgac gacacagctt gcgagaccga
     2521 gctgataggc gcggtcggcg ccgatgaagc ggcccgtcaa catgaactcc gccgccaccg
     2581 aactgccgac catgcggggc aggaagtagc tgaccccgat gtcgcaggcg ctgaggccga
     2641 tgcggatgaa ggcggcgttc atgcgcgtct tgggggtgac gatccgcacg tcgctggcca
     2701 gggccagggc gaagccgccg ccggccgccg cgccgtcgac acaggcgatg atcggctgcg
     2761 gacagcggcg catggcgatg acgatctcgg cgatgctgcg ctgggaatcg aggccgtcgg
     2821 cgatgcggcg ctggccgttg ctgtcgcttc gctccttcag gtccaggccg gcgcagaagg
     2881 ccgggccggc gccggacagc accaccacac ggacatcccg ccgccagtag agattcacga
     2941 acaggtcgcg cagctcggcc gtcaggcggc ggttcagggc gttgaggctg tcgggccggt
     3001 tcatcgtcac ccagagggcg tcgccctcct gccgcagctc cagatgctca tagggctccg
     3061 acgccatggt gatggtcacg ctcatcgggt ctgtcctcca tcgacgggca c
//
LOCUS       DAORNX010000358         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23679_length_3108_cov_5.743531, whole genome shotgun sequence.
ACCESSION   DAORNX010000358 DAORNX010000000
VERSION     DAORNX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23679_length_3108_cov_5.743531"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>224
                     /locus_tag="PLO65_13565"
     CDS             <1..>224
                     /locus_tag="PLO65_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD29322.1"
                     /translation="AIAQARASLDTSRTNLAKATIRSPVDGVVLARSIEPGQTVAASL
                     QAPTLFVLAEDLTRMELQVDIDEADVGQAV"
     assembly_gap    225..442
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <443..719
                     /locus_tag="PLO65_13570"
     CDS             <443..719
                     /locus_tag="PLO65_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29323.1"
                     /translation="PAAALRFSPPATTTASGQTGLVASLTPRMPAMGGRRRSNGANAV
                     QQVWTLRDGEPVALPVTVGASDGQWVEITGAGLTPGLLLITGTDEPE"
     gene            716..1420
                     /locus_tag="PLO65_13575"
     CDS             716..1420
                     /locus_tag="PLO65_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD29324.1"
                     /translation="MSGPLIQLSGVTKTFGEGEAAFQALRGIDLAVGEGEFVAIMGPS
                     GSGKSTAMNILGCLDVPTTGSYRFRGVAVDRLDRRQRALLRRRHLGFVFQGYNLLPRT
                     TALENVELPLLYRGVSRVERAALARATLAKVGLTGWEHHTPAKLSGGQQQRVAIARAI
                     VTRPTVLLADEPTGNLDSEKGREIMELLAALNRDQGITILMVTHEPDMAAYARRVVQF
                     HDGRIRSDSGLGASTA"
     gene            1420..2622
                     /locus_tag="PLO65_13580"
     CDS             1420..2622
                     /locus_tag="PLO65_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD29325.1"
                     /translation="MLLNALKLALDAIRRNLLRASLTVLGVVIGVAAVVTMVTLGNGA
                     TRAVSNQISSLGSNLLMVRTGQRMGPPGLGVPGFRTADVAAIVVAVPTIAAASPVDTT
                     SATLVYGSQNWSTSVAGVSRPYFAARDWSLASGRLFTEAEERAGRAVCVVGDTIRREL
                     FAGRDPVGARLRVRQFSCEIIGLLKPRGQAAMGQDQDDTVILPLRTVQRRLTGRQDVN
                     SITLSVRDGASTARAVAQVEAVLRERRKIGPGEEDNFSVMDTKQIAETLAGTTRVMTM
                     LLGAVAAVSLLVGGIGIMNIMLVSVTERTKEIGVRLAIGALEQDVLLQFLVESVVLAA
                     FGGLAGVLIAAAASVILARVIQVPFLFDPGINLLAFAVAVIIGVVFGFFPARRAARLD
                     PIEALRRE"
     gene            2690..>3108
                     /locus_tag="PLO65_13585"
     CDS             2690..>3108
                     /locus_tag="PLO65_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD29326.1"
                     /translation="MKTRAAVAFEARKPLEIVEVDLEGPRAGEVLVEIKATGICHTDA
                     YTLDGLDSEGIFPSILGHEGAGVVLEVGAGVTSVVPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGQMPDGTSRFSYKGQPIAHYMGCST"
BASE COUNT          412 a         1034 c         1021 g          423 t
ORIGIN      
        1 cggcgatcgc ccaggcgcgg gccagtctcg acaccagccg cacaaacctg gccaaggcga
       61 cgatccgctc gcccgtcgac ggcgtggtcc tggcgcgcag catcgaaccg ggccagaccg
      121 tcgcggccag cctgcaggcg ccgaccctgt tcgtcctcgc cgaggacctg acccgcatgg
      181 agctgcaggt cgacatcgac gaggccgacg tgggccaggc ggtgnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nngcccgccg cggccttgcg cttcagtccg cccgccacca
      481 cgacggccag cggccagacc ggcctggtcg ccagcctgac cccgcgcatg ccggccatgg
      541 gcggccgccg ccggagcaat ggcgccaatg cggtccagca ggtgtggacg ttgcgtgacg
      601 gcgagcccgt ggccctgccc gtcacggtcg gcgccagcga cggccagtgg gtcgagatca
      661 ccggcgccgg gctcacgccc gggctcctcc tgatcaccgg aacggacgag cccgaatgag
      721 cggcccgcta atccagctgt cgggcgtgac caagaccttc ggtgagggcg aggcggcgtt
      781 ccaggccctg cgcggcatcg atcttgccgt cggtgagggc gagtttgtcg ccatcatggg
      841 ccccagcgga tcgggcaagt cgacggcgat gaacatcctc ggctgcctcg atgtcccgac
      901 caccggcagc taccggtttc gcggtgtcgc cgtcgacagg ctggaccggc ggcagcgggc
      961 gctgctgcgc cgccgtcacc tggggttcgt gttccagggc tacaacctgt tgccgcgcac
     1021 cacggcgctc gagaacgtcg agctgcccct gctgtatcgt ggcgtctccc gcgtcgagcg
     1081 cgcggccctg gctcgcgcca ccctggccaa ggtcgggctg accggttggg aacaccatac
     1141 cccggccaag ctgtccggcg gtcagcagca gcgggtggcc atcgcccggg ccatcgtcac
     1201 ccggccgacc gtgctgctgg ccgacgagcc gaccggcaac ctcgacagcg aaaagggccg
     1261 ggaaatcatg gagctgctcg cggcgctcaa ccgcgaccag gggattacca tcctgatggt
     1321 cacccacgag ccggacatgg ccgcctacgc ccgtcgcgtc gtgcagttcc acgacgggcg
     1381 catccggtcc gactcgggtc tgggcgcgag cacggcctga tgctgctcaa cgccctcaag
     1441 ctggccctcg acgccatccg ccgcaacctg ctgcgggcct cgctgacggt gctgggcgtg
     1501 gtcatcgggg tggccgcggt ggtcaccatg gtcaccctcg gcaacggcgc gacgcgggcc
     1561 gtgtcgaacc agatctcgag cctcggcagc aacctgctga tggtccgcac gggccagcgg
     1621 atgggtccgc cgggcctcgg cgtgcccggc ttccggaccg ccgacgtggc ggccatcgtc
     1681 gtggccgtgc cgaccatcgc ggcggcctcg ccggtcgaca cgacatccgc gacgctggtt
     1741 tacgggtcgc agaactggag cacctcggtg gccggggtgt cgcgacccta tttcgcggcc
     1801 cgcgactggt cgctggcctc gggccggctg ttcaccgagg cggaggagcg cgccggccgc
     1861 gccgtctgcg tggtcggcga caccatccgg cgggaactgt tcgccggccg cgatccggtc
     1921 ggcgcgcggc tgcgggtgag gcagttctcc tgcgagatca tcggcctgct caagccgcgc
     1981 ggccaggcgg ccatgggcca ggaccaggac gacacggtca tcctgcccct gcgcacggtg
     2041 cagcgccgcc tgaccggccg ccaggatgtc aactccatca cgctgtccgt gcgcgacggc
     2101 gcctcgaccg cgcgggcggt ggcccaggtg gaggccgtgc tgcgcgaacg ccgcaagatc
     2161 ggcccggggg aggaggacaa cttcagcgtc atggacacca agcagatcgc cgagaccctc
     2221 gccggcacga ccagggtgat gacgatgctg ctcggcgcgg tggcggcggt cagcctgctg
     2281 gtcggtggca tcggcatcat gaacatcatg ctggtgtcgg tcactgaacg gaccaaggag
     2341 atcggcgtgc gcctggccat cggcgcgctg gagcaggacg tgctgctgca gttcctcgtc
     2401 gagtcggtgg ttctggcggc cttcggtggc ctggccgggg tgctgatcgc cgccgccgcc
     2461 tcggtgattc tggcccgggt catccaggtg ccgttcctgt tcgaccctgg catcaacctg
     2521 ctggccttcg ctgtggcggt gatcatcggc gtggtgttcg gcttcttccc ggcccggcgc
     2581 gcggcccggc tcgacccgat cgaggccttg cggcgggaat agcggcgacg ggcggtggac
     2641 gcgcgcggtc cgctcgggga ttatcccggc cgcctcaccg gagattctca tgaaaacccg
     2701 cgccgccgtc gcgttcgaag ccaggaagcc gctggaaatc gtcgaggtcg acctcgaggg
     2761 gccgcgcgcg ggcgaggtgc tggtcgagat caaggcgacc ggcatctgcc acaccgacgc
     2821 ctatacgctc gacggcctcg acagcgaggg catcttcccc tcgatcctcg gccatgaagg
     2881 cgcgggcgtg gtgctggagg tcggcgcggg ggtgaccagc gtggtccccg gcgatcacgt
     2941 gatcccgctc tacacgcccg aatgccggca gtgcaaaagc tgcctcagcc gcaagaccaa
     3001 cctctgcacg gccatccgcg ccacccaggg caagggccag atgccggacg gcaccagccg
     3061 cttctcctac aagggccagc cgatcgccca ctacatgggc tgctcgac
//
LOCUS       DAORNX010000359         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23696_length_3106_cov_5.162242, whole genome shotgun sequence.
ACCESSION   DAORNX010000359 DAORNX010000000
VERSION     DAORNX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23696_length_3106_cov_5.162242"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLO65_13590"
     CDS             complement(<1..819)
                     /locus_tag="PLO65_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29327.1"
                     /translation="MTDRILPPLSADQKARVRKEILRFNHRLEDTGLFTDAALARLID
                     EHPRDQVTICTMQPDPPAEEPWIAGEAADLDGAALVEAARTGALWVSPRSAMTRNPRY
                     KVVFDQLMGEFSKETGLPVLTADAAVLISSPRMGIFFHVDPAETMLWHIRGHKTIHVY
                     PPRQDIVTEAALEAILLKENLSDLPWDPAFEAQGVAEALAPGQAAFWPQHSPHRVVNG
                     DDLNVSISVEFSSPQSMLTNSVFYTHGRLRRTVGWAPASRGPAQVLAPAYLLAAK"
     assembly_gap    888..998
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<999..1220)
                     /locus_tag="PLO65_13595"
     CDS             complement(<999..1220)
                     /locus_tag="PLO65_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29328.1"
                     /translation="MLLSTLMLAGATAFGHGPAADQRPFRASRADAIIAAATMQGADA
                     PPAEATPVRYPSADGDILADLGPVMTVPTP"
     gene            complement(1619..>3106)
                     /locus_tag="PLO65_13600"
     CDS             complement(1619..>3106)
                     /locus_tag="PLO65_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRD29329.1"
                     /translation="AEVDNILRFERDFPGARVIRLERNYRSTSHILAAASGLIAVNKG
                     RLGKTLWTEGDEGEKVRVRGVWDGEAESRLIAEEIERWKKGGRKYRDAAILVRASFQM
                     RAFEERFVMLQIPYTVVGGPRFFERAEIRDAHAYLRLVQSPDDDLAFERIINTPKRGI
                     GDTTVQKILQIARGGSVSAVTAARGIVQTDELAARTRTALANFIRDLDRWSHLSATGT
                     HHDRLTETILEESGYTDALRLDKGPTSQTRLENLKELVQSMSAFETLSDYLEHVSLVM
                     DLDRGGAGDSVQIMTLHAAKGLEFPLVFLPGWEEGVFPSQRSLDEKGEKGLEEERRLA
                     YVGVTRAREEARISFVANRMVYGRWTSQLPSRFVDELPLANVEASSETGYYGGGPGMS
                     QPTSSRWDDDRTFGAGYSSPGWKRAQERGYHGGPAGRTQVLEGEGRLVAVSESSAASD
                     YKRGDRVFHIKFGYGKVTAVEGNKLTVDFDKAGVKKVLDGFVEKG"
BASE COUNT          410 a         1022 c         1067 g          496 t
ORIGIN      
        1 cttggccgcc agcagatagg ccggcgccag gacctgggcc gggccgcgcg aggctggcgc
       61 ccagccgacc gtccgccgca gtcggccatg ggtgtagaag acgctgttgg tcagcatcga
      121 ctgcggcgag gagaactcca ccgagatcga cacgttcagg tcgtcgccgt tgaccacccg
      181 gtgcggcgaa tgctgcggcc agaaggcggc ctggcccggc gcgagggcct cggccacgcc
      241 ctgcgcctcg aacgccgggt cccacggcag gtcggacagg ttttccttga gcaggatcgc
      301 ctccagcgcc gcctcggtga cgatgtcctg gcgcggcgga tagacgtgga tcgtcttgtg
      361 gccgcggata tgccacagca tcgtctcggc cgggtcgacg tggaagaaga tgcccatgcg
      421 cgggctggag atcagcaccg ccgcgtcggc ggtcagcacc ggcaggccgg tctccttcga
      481 aaattcgccc atcagctggt cgaacaccac cttgtagcgc gggtttctcg tcatggcgct
      541 gcgcggcgag acccacaggg cgccggtgcg ggccgcctcg accagcgccg ccccgtccag
      601 gtcggccgcc tcaccggcga tccacggctc ctcggccggg ggatcgggct gcatggtgca
      661 gatcgtcacc tggtcgcgcg gatgttcgtc gatcagccgg gccagcgcgg cgtcggtgaa
      721 caggccggtg tcctccagcc ggtggttgaa gcgcaggatc tccttgcgga cgcgcgcctt
      781 ctggtcggcg gaaagcggcg gaagaatgcg gtcggtcatg cccagtgtcc caatgcggcg
      841 caggtccggg gacatgcagc aagggcctgg ccagtcgggg cgttaccnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcgtcggaac ggtcatgacg
     1021 ggccctagat ccgccaggat gtccccgtcg gcgctcggat agcgaaccgg ggtggcctcg
     1081 gccggcgggg cgtcggcccc ctgcatggtc gccgcggcga taatcgcgtc ggcccggctg
     1141 gcccggaagg ggcgctggtc ggcggcgggc ccatgcccga aggccgttgc gccggcgagc
     1201 atcagggttg aaagcagcat gggtcgctcc gtcacgcggt cgtcgcaggc acagcgcgga
     1261 aggcggccgt tggttgcggc gcggcggcgg ccggtaacgg ctcgactgtc tcgtatgcgc
     1321 tggtccttct ccccttgcgg gagaaggtgg cctgcgaagc aggccggatg aggggttgaa
     1381 ggcgggatcg gcgtcgcaac gaccgcccgc caaccgttca agccggcgag acccctcatc
     1441 cgtcaggctc cgcctgacac cttctcccac aaggcgagaa gggggccgaa tatgcatccc
     1501 tgcattttat gggcttccgt cgcgatgatg cgccgattgc ccgacgattt ggggcggcgg
     1561 tcccgcgaat gtcccgcctt gtcccgggat tacccgaccc gggagtctcg cgccgccatc
     1621 accccttctc gacgaacccg tcgaggacct ttttcacgcc ggccttgtcg aagtcgacgg
     1681 tcagcttgtt gccctcgacg gcggtgacct tgccgtagcc gaacttgatg tggaacacgc
     1741 ggtcgccgcg cttgtagtcg ctggcggcgg agctttccga gacggcgacc agcctgcctt
     1801 cgccctcgag cacctgggtg cggccggccg ggccgccgtg atagccgcgc tcctgggcgc
     1861 gcttccagcc gggcgaggag tagccggcgc cgaaggtgcg gtcgtcgtcc cagcggctgg
     1921 aggtcggctg ggacatgccc gggccgccgc cgtagtagcc ggtctcgctg ctggcctcga
     1981 cgttggccag cggcagctcg tcgacgaagc ggctgggcag ctggctggtc cagcggccgt
     2041 agaccatgcg gttggcgacg aagctgatgc gcgcctcctc gcgggcgcgg gtcacgccga
     2101 cgtaggccag gcggcgttcc tcctcgaggc ccttctcgcc cttctcgtcc aggctgcgct
     2161 ggctggggaa cacgccttcc tcccagcccg gcaggaagac cagcgggaac tccagccctt
     2221 tggccgcgtg cagcgtcatg atctgcaccg agtcacccgc gccgccgcgg tccaggtcca
     2281 tgaccagcga gacgtgctcc agatagtccg acagggtctc gaaggcgctc atcgactgga
     2341 ccagctcctt gaggttttcc agccgggtct ggctggttgg tcccttgtcg agccgcaggg
     2401 cgtccgtgta gccgctctcc tcgagaatgg tctccgtcag ccggtcatgg tgcgttccgg
     2461 tcgccgacag gtggctccag cggtccaggt cgcggatgaa gttggccagg gccgtgcggg
     2521 tgcgggcggc cagctcgtcg gtctggacga tgccgcgggc cgccgtcacc gcggagacgc
     2581 tgccgccgcg ggcgatctgc aggatcttct gcaccgtggt gtcgccgatg ccgcgcttgg
     2641 gcgtgttgat gatccgttcg aaggccaggt cgtcgtccgg cgactggacc agccgcagat
     2701 aggcgtgggc gtcgcggatc tcggcccgct cgaagaagcg cgggccgccg accacggtgt
     2761 aggggatctg cagcatgacg aagcgttcct cgaacgcccg catctggaag ctggcccgca
     2821 ccaggatggc cgcgtcgcgg tatttgcggc cgcccttctt ccagcgctcg atctcctcgg
     2881 cgatcagccg gctctcggcc tcgccgtccc agaccccgcg cacgcggacc ttttcgccct
     2941 cgtcgccctc ggtccacagg gtcttgccca gacggccctt gttgaccgcg atcaggccgc
     3001 tggcggcggc caggatgtgg ctggtcgaac gatagttgcg ctccagccgg atcaccctgg
     3061 cgcccgggaa gtcgcgctcg aagcgcagga tgttgtccac ctccgc
//
LOCUS       DAORNX010000360         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_23737_length_3102_cov_7.261241, whole genome shotgun sequence.
ACCESSION   DAORNX010000360 DAORNX010000000
VERSION     DAORNX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23737_length_3102_cov_7.261241"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..731)
                     /locus_tag="PLO65_13605"
     CDS             complement(66..731)
                     /locus_tag="PLO65_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD29330.1"
                     /translation="MITLYHCADARSFRPLWALEELGLDYDLRMLPFPPRVLKREYLQ
                     ENPLGTIPLLVDGETRMTESSAIVEYLSRRYGAAGGLAVEPDEPGFGAWLNALHFGEA
                     TLTFPQTLVLRYRRLEPEARRQPQVADDYAKWFLARLRGLEAIVSESEYVAAGRFTGG
                     DISVAYALLLARSLGLADQFPPAVAAYWEHMQAREGYQRARAAQKRAAADQALTTVPL
                     DQL"
     gene            939..2633
                     /locus_tag="PLO65_13610"
     CDS             939..2633
                     /locus_tag="PLO65_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD29331.1"
                     /translation="MRRWGLFAAGWLLILAGSWLAGAVQTSGGITVTDVRFPGTGGVT
                     MSGLLYTPRTATAEHPAPGVLAVHGYINSRETQSPFAIEFARRGYVVLALDQTGHGYS
                     GGAAFANGFGGPDGLKYLRGLPMVDKANIGLEGHSMGGWTVLAAAAAMPDAYKAVVLE
                     GSSTGAPFAAEGTPAWPRNLALVFSRYDEFSKLMWGVERARDLAGSAKLKTVFGTSAD
                     VVPGKVYGAIADGTARRLTQPVTTHPGDHLSPAAVGDAVDWFARTLTGGAPRPASDQI
                     WMWKEIGTGIAFVGFVVLLLGTFNGLLGLPLFAALRHEPEAAVQWRSGRWWGGLLTMA
                     LLPVVTYFPVFIAVTVLLKPSPLLPQTITSQVVAWALVNAVLTLAVGWLMRRRNLASF
                     DNRWGPAVLIAVATTSIGYLSLVAVDALFKVDFRFWVVALKLFSPAQAKIALVYVLPL
                     TAFFLVATRALHGRLSVLGDGPVKAYASAVAAMALGFALLLVVDYGLFFLTGRLPTDL
                     DPLSTVVAIQFLPLMAIVGVISTFTWRRTGSYVPGAVICGLFVTWYVVAGTATQVV"
     gene            complement(2716..3009)
                     /locus_tag="PLO65_13615"
     CDS             complement(2716..3009)
                     /locus_tag="PLO65_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD29332.1"
                     /translation="MDRLYYVYILASRRNGTLYVGVTNDLSRRMRQHREGTASAFTKK
                     YGVTMLVWYEHFYQILDAIACEKKLKRWRRAWKLARIEAFNPEWRDLYETWNQ"
BASE COUNT          466 a         1064 c         1058 g          514 t
ORIGIN      
        1 ccagatcgac gaagccggcg gcggccagcc gctggtgctc aacgtgcgcg gcgaggcatg
       61 acccttcaga gctggtccag cggcacggtc gtcagggcct ggtcggccgc cgcgcgcttc
      121 tgggccgccc gggcgcgctg gtatccctcc cgcgcctgca tgtgttccca ataggccgcc
      181 acggccggcg ggaactggtc ggccaggccc agcgaccggg ccagcaacag ggcgtatgcg
      241 acagagatgt ccccgccggt gaagcgaccg gccgccacgt attcggactc gctgacgatc
      301 gcttccagcc cccgcagccg ggccaggaac cacttggcgt agtcgtcggc gacctgcggc
      361 tggcggcgcg cctccggctc cagccgacgg tagcgcagca ccagggtctg cggaaacgtc
      421 agggtcgcct cgccgaaatg cagggcgttg agccaggcgc cgaagcccgg ctcgtcgggc
      481 tcaacggcca agccgcctgc cgcgccatac ctgcgcgaca ggtattcgac gatggccgag
      541 gactcggtca tccgcgtttc gccgtcgacc aacagcggga tcgtgcccag cgggttctcc
      601 tgcagatact cccgcttcag gacccttggc gggaacggca gcatgcgcag gtcgtagtcc
      661 agccccagct cctccagcgc ccacagcggc cggaacgagc gcgcgtcggc gcagtggtag
      721 agggtgatca tggcgcgggt tcccgattct gacgcttaaa ggtaggccat gctgcgtcct
      781 tcgacacggc ccttcgggcc tgctcaggat gacgtgaagg ggcggacctc aaaggtccac
      841 gtcatggtga gcagcgccga aggcgcgtgt cgaaccacgc attgtgccgg cgaccactcc
      901 gggcaatgct cgcgcataac aggacggggg agacgacgat gcgcagatgg gggctgttcg
      961 cggccggatg gctgctgatc ctggccgggt cgtggctggc cggcgccgtg cagacgtcgg
     1021 gcgggatcac cgtcaccgac gtgcgcttcc cgggaaccgg cggcgtgacc atgagcggcc
     1081 tgctctacac gccccggacc gccacagccg aacaccccgc gcctggcgtg ctggccgtgc
     1141 acggctacat caacagtcgc gagacccaga gcccgttcgc catcgaattc gcccggcgcg
     1201 gctacgtcgt actggccctt gaccagaccg gccacggcta cagcggcggc gcggccttcg
     1261 ccaacggctt cgggggcccg gacgggctga aatacctgcg cggcctgccc atggtcgaca
     1321 aggccaacat cggcctcgaa ggccacagca tgggcggctg gacggtgctg gccgccgccg
     1381 ccgccatgcc cgacgcctac aaggccgtgg tgctggaggg ctcctccacc ggcgcgccgt
     1441 tcgcggccga gggcacaccg gcctggccgc gcaacctcgc gctggtgttc agccgctacg
     1501 acgagttcag caagctgatg tggggggtcg agcgggcgcg ggacctggcc ggcagcgcca
     1561 agctgaagac ggtgttcggg acctcggccg acgtcgttcc cggcaaggtc tacggcgcca
     1621 tcgccgatgg cacggcccgg cggctgaccc agccggtcac cacccatccc ggcgaccacc
     1681 tctccccggc cgccgtcggc gacgcggtgg actggttcgc caggaccctg accggcggcg
     1741 cgccgaggcc ggcgtccgac cagatctgga tgtggaagga gatcggcacg ggaatcgcct
     1801 tcgtcggctt cgtcgtgctg ctgctgggga cgttcaacgg cctgctcggc ctgcccctgt
     1861 tcgcggcgct gcgacacgag cccgaggcgg ccgtgcaatg gcgctcgggc cgctggtggg
     1921 gcgggctgct gaccatggcc ctgctgccgg tggtgacata cttcccggtg ttcatcgccg
     1981 tgacggtgct gctcaagcct tcgccgctgc tgccgcagac catcaccagc caggtggtcg
     2041 cctgggcgct ggtcaacgcg gtgctgacgc tggccgtcgg ctggctgatg cggcggcgca
     2101 acctggccag cttcgataac cgctgggggc cggccgtgct gatcgcggtg gcgaccacga
     2161 gcatcggcta cctgtcgctg gttgccgtcg acgccctgtt caaggtcgac ttccgcttct
     2221 gggtggtggc gctcaagctg ttcagtccgg cccaggcgaa gatcgccctg gtctatgtcc
     2281 tgcccctgac cgccttcttc ctcgtcgcca cgcgcgccct gcacgggcgg ctgtcggtgc
     2341 tcggcgacgg ccccgtgaag gcctacgcct cggccgtcgc ggccatggcg ctgggctttg
     2401 cgctgctgct ggtggtcgac tacggcctgt tcttcctgac cggccgcctg ccgaccgacc
     2461 tcgatccgct gagcacggtg gtggcgatcc agttcctgcc gctgatggcc atcgtcggcg
     2521 taatctcgac cttcacctgg cggcggaccg gaagctatgt gccgggcgcg gtgatctgcg
     2581 ggctgttcgt cacctggtac gtggtggcgg ggacggcgac gcaggtggtc taaagcgccc
     2641 caagaactgt catcccggcc gaagcgaagc ggagagccgg gacccagatc tagtctgatc
     2701 ccgcccccgt tgcagctact ggttccaggt ctcgtagagg tcgcgccatt caggattgaa
     2761 ggcttcgatc cgcgcaagct tccaggcccg ccgccagcgt ttcagcttct tctcgcaggc
     2821 gatggcgtcg agaatttgat agaaatgctc gtaccagacg agcatggtca cgccgtattt
     2881 cttagtgaag gccgaggccg tcccttctcg atgttgccgc atccggcggg acaggtcgtt
     2941 ggtcaccccg acatagagcg tgccatttcg tcggctcgcg agaatgtaga catagtagag
     3001 tcgatccatg atacgacttt aacgattgtc accagaaaaa tctccttccc aatcactgtc
     3061 atcccggccg cagcgtagcg gagagccggg acccagattt gg
//
LOCUS       DAORNX010000361         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24112_length_3065_cov_6.251163, whole genome shotgun sequence.
ACCESSION   DAORNX010000361 DAORNX010000000
VERSION     DAORNX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24112_length_3065_cov_6.251163"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..712)
                     /locus_tag="PLO65_13620"
                     /pseudo
     CDS             complement(1..712)
                     /locus_tag="PLO65_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(767..946)
                     /locus_tag="PLO65_13625"
     CDS             complement(767..946)
                     /locus_tag="PLO65_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29333.1"
                     /translation="MASPGKRAFLLRVRPEVLEAVERLAAAELRSVNAQLEVLLREAL
                     SRRNGGRFGKEPEDR"
     gene            complement(950..1843)
                     /locus_tag="PLO65_13630"
     CDS             complement(950..1843)
                     /locus_tag="PLO65_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD29334.1"
                     /translation="MAEHGSINRTTERQGGGVNGGLILLLMLALLAAGIWLLSHIERD
                     GGELVGIAGIVVIVIAALGFAGFYSLQPNEGAAITLFGAYRGTDRSTGLRWVLPWFGR
                     KKISLRVRNVTSEMLKVNDKRGNPIEIAANVVWRVEDSAQALFDVDDYAAFINIQIET
                     ALRETARHYAYDHAEHEEPTLRADAEIVGERLKVDLQDRVAVAGIAIDETHLMHLAYA
                     PEIAGAMLRRQQAEAVIAARRTIVIGAVSMVEDALQQLGDRGVVNLDEERRAAMVSNL
                     LVVLCGDRDAQPVLNTGTLYG"
     gene            1949..>3065
                     /locus_tag="PLO65_13635"
     CDS             1949..>3065
                     /locus_tag="PLO65_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD29335.1"
                     /translation="MARYLFTTWEGGGHVQPMMLVARGLVDRGHQVLLLSDACNAPEA
                     AAFGLPFRPWRHAPSRPDKTAASDLLRDWEADNPAEGIQRLCERIIAGPAARYARDVL
                     EALGTAPFDAVVTQELLFGAMMAAESAGVPLALFCANVWPFPTLPGLPPFGAGMAPAT
                     GEPDVLLHQMVAQTTRMLFQTGLPDLNLARGSLGLPPLADLFDQIDAARRILLATSRD
                     FDFAPDPLAEPFRYVGPYVADPVDAGAWVSPWSPNDPRPLVVVTSSGLYEAQEGLLRR
                     IIAALASLPVRALVTLGPALDPADFPGADNVEIRPSAPHGLLFSEAALFVTHGGHGST
                     IRPLTAGLPLLVTPGLRDQRDNAVRVTARGASLMLERD"
BASE COUNT          430 a         1086 c         1081 g          468 t
ORIGIN      
        1 cgatcagcag caacacatag ggctcgccca ggcgctcgac ggcctcgacc agcgcgtcga
       61 tgcgcttctc ccgggcggga cgcccggcga acaccagcag gcgatggcgg ggttcgagcc
      121 ccagctcccg gcgcacggcc tcccggtcgg cgcgccccgg gtgaaacacc tcggtatcga
      181 ccccgagcgg cagggcgatg gccgacgtca cgcccgactc gatcaggcga ccggcgacgt
      241 accggctcgg cgccacggcc tgatcgaaat cgcggtagat cgccgcccag cgtcgccgca
      301 ccggcttctc ggcccagtcg ccgatcatca gccctgccag ggcccccaga tcggtgtgac
      361 agaagccgac gaccggcacc cccagccgcg cgcccgcctt cagcgcggcg agccccggcg
      421 tgtaggggtc gcccgcctcg ataatggacg ggttctggcg gatcagccgg tccatccacg
      481 tcgccttgct cagtggccag cgatagccgt cgccgaacgg caacggcgcg gcgtagatcg
      541 acacccggcc gtggccgtca tggaagtccc gcggtccggg ggcgaccagg gtgtggcgaa
      601 cgccgggccg ggccctggcc agccaggcgc gcttggcgtt gaggtagcgc ttcacgccgc
      661 cgctgcgcgg ggcgtagagc atggtggtgt cgaccaggcg gggacagggt tcggcggacg
      721 gcaacggcag gatcccggcg ccggggcggc gcttccgaac aaagcctcag cggtcctccg
      781 gttccttgcc gaaccggccg ccgttacgcc gcgacagggc ttcccgcagg agcacctcga
      841 gttgcgcgtt gacgctgcgc agttcggcgg ccgcgagacg ctcgaccgcc tcgagcacct
      901 cgggcctcac ccgcagaaga aaggcgcgtt ttcccggact cgccacgcct cacccgtaga
      961 gcgtgccggt attgagcacg ggctgggcgt cgcggtcgcc gcagagcacg accagcaggt
     1021 tcgagaccat cgccgcgcga cgctcctcgt ccaggttgac gacgccccgg tcgcccagct
     1081 gctggagggc gtcctcgacc atgctgaccg cgccgataac gatcgtccgg cgggctgcga
     1141 tgacggcctc ggcctgttga cggcgcaaca tggccccggc gatctcgggc gcgtaggcca
     1201 ggtgcatgag gtgggtctcg tcgatggcga tgccggccac ggcgacccgg tcctgcagat
     1261 cgaccttgag ccgctcgccg acgatttcgg cgtccgcccg cagggtcggc tcctcatgct
     1321 cggcatggtc gtaggcgtag tgccgcgccg tctcgcgcag ggcggtttcg atctggatat
     1381 tgatgaaggc ggcgtagtcg tcgacatcga acagggcctg ggccgagtcc tcgacgcgcc
     1441 agacgacgtt ggccgcgatc tcgatcggat taccgcgctt gtcgttgacc ttgagcatct
     1501 cgctggtgac gttgcgcacg cgcaggctga tcttcttgcg gccgaaccag ggcagcaccc
     1561 agcgcaggcc ggtgctgcga tcagtgcccc tgtaggcgcc gaacagggtg atggccgcgc
     1621 cctcgttcgg ttgcaggctg tagaagccgg cgaagccgag ggcggcgatg acgatcacca
     1681 cgatgcccgc gatcccgacc agttcgccgc cgtcccgttc gatgtggctg agcagccaga
     1741 ttcccgccgc cagcaaggcc agcatgagca gcaggatgag cccgccgttg acgccgccgc
     1801 cctgccgttc cgtggtgcgg ttgatgcttc cgtgttcggc catcggcccc tccatatctg
     1861 gtatcaaagt gatatcacct tgatccaacc tgtcaacgcg cttgccgagc aggcgggcga
     1921 ggcgctagcc aagcggcggg gggaagtcat ggcccgatat ctgttcacga cctgggaagg
     1981 cggcgggcat gtgcagccga tgatgctggt cgcccggggg ttggtcgaca ggggccacca
     2041 ggtgctgctg ctcagcgacg cctgcaacgc gccggaggcg gcggcgttcg gcctgccctt
     2101 ccggccctgg cgccacgcgc cgtcacggcc tgacaagacc gccgccagcg acctgctgcg
     2161 cgactgggag gccgacaatc cggccgaagg catccagcgc ctgtgcgagc ggatcatcgc
     2221 agggccggcg gcgcgttacg cgcgcgatgt gcttgaggcg ctcgggacgg cgccgttcga
     2281 cgcggtcgtc acccaggagc tgctgttcgg cgcgatgatg gcggcggagt cagccggggt
     2341 tccgctggcc ctgttctgcg ccaacgtctg gcccttcccc accctgcccg gcctgccgcc
     2401 gttcggcgcg ggcatggcgc cggcgacggg ggagccggac gtcctgctgc accagatggt
     2461 cgcgcagacg acacgaatgc tgttccagac cggcttgccg gacctgaacc tggcgcgtgg
     2521 aagccttggc ctgccgccgc tggccgacct gttcgaccag atcgacgccg cccggcggat
     2581 tctgctggcc accagccgcg atttcgactt cgcgcccgac ccgctggccg agccgttccg
     2641 ctatgtcggt ccctatgtcg ccgatcccgt ggacgccggg gcctgggttt cgccctggtc
     2701 gccgaacgac ccccggccgc tggtggtggt cacctcgtcc ggactctacg aggcgcagga
     2761 aggacttctg cggcggatca tcgctgcgct ggcgagcctg cccgtccggg ctctggtgac
     2821 cctgggcccg gcgctggacc cggccgattt ccctggcgcg gataacgtgg agatccggcc
     2881 cagcgcgccg cacggcctgc tgttcagcga ggccgccctg ttcgtgactc atggcggcca
     2941 cggctcgacg atccggccgc tgaccgccgg cctgccgctg ctggtgacgc ccggcttgcg
     3001 cgatcagcgg gacaacgccg tgcgggtcac ggcgcgcggc gcgagcctga tgctggagcg
     3061 ggacg
//
LOCUS       DAORNX010000362         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24205_length_3057_cov_6.786809, whole genome shotgun sequence.
ACCESSION   DAORNX010000362 DAORNX010000000
VERSION     DAORNX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24205_length_3057_cov_6.786809"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLO65_13640"
     CDS             <1..746
                     /locus_tag="PLO65_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD29336.1"
                     /translation="SRFSYKGQPIAHYMGCSTFANHTVLPEIAVAKIRKDAPFDKACY
                     IGCGVTTGVGAVTNTAKVEPGANCIVFGLGGIGLNVIQGLKLVGANMIVGVDINPDRE
                     AWGRQFGMTHFVNPKTVDGDVVPHLVALTDGGADYTFDATGNVTVMRQALEACHRGWG
                     ESIIIGVAEAGREIATRPFQLVTGRVWKGTAFGGARGRTDVPRIVDWYMDGKIQIDPM
                     ITHTMPLEDINKAFDLMHAGESIRSVVVF"
     gene            872..1708
                     /gene="fghA"
                     /locus_tag="PLO65_13645"
     CDS             872..1708
                     /gene="fghA"
                     /locus_tag="PLO65_13645"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRD29337.1"
                     /translation="MVEILSTHRTFEGTLHYCRHQSAATGTPMRFTIFLPPGEGPFPV
                     LVWLSGLTCTEENFTTKGGAYGAAAALGLAIVAPDTSPRGDEVADHPDYDFGKGAGFY
                     IDATEKPWAPHFTMETYIVRDLLEAVHGAFPLDPARRAISGHSMGGHGALTLALKHPD
                     LFRSVGAFAPICSPTRCPWGRRAFTGYLGVDETAWAGHDATLLIAAGAAKGRYDDILV
                     EQGTADPWLAEQLKPELLEAACEAAGQPLTVLRHEGYDHGYFFVATFIADHLAWHRER
                     LE"
     gene            complement(1714..2994)
                     /locus_tag="PLO65_13650"
     CDS             complement(1714..2994)
                     /locus_tag="PLO65_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRD29338.1"
                     /translation="MTLNIGPFEAASIVIVAAAALGYVNHRFIRLPQTIALSLMGALA
                     SVVVAVVDFLLPGGQLGDGVRTFLDQVDFKVALLDGMLSFLLFAGALHVDIRQLKIGR
                     WPIAVLSSLGLLLSTVIVAAGLHLLSRLLGAEISLAWCLVFGALISPTDPVAVMALLA
                     RSDAPRTLKATVAGESLFNDGVGVVLFSILLAAAVGGVEITPLKGAEMFVVEAFGGAA
                     VGLVLGWVAFLAMRSIDDYVLEVLISLALVMGGYSLAHLLHVSGPVAMAVGGLVIGNH
                     GVAMAMSDTTRDHLLKFWDLIDHILNAALFLLIGLEGVVLLGNAGVALLGLAAIPLTL
                     IARAASVAGPLLIWRRILPFGQAFPLLTWGGLRGGISIALALSLPPSPWRDALLAATW
                     LVVMFSVVVQGATVERLMRRQASRTAPEDSPPAS"
BASE COUNT          552 a         1083 c         1023 g          399 t
ORIGIN      
        1 ccagccgctt ctcctacaag ggccagccga tcgcccacta catgggctgc tcgaccttcg
       61 ccaaccacac ggtgctgccg gagatcgcgg tggcgaagat ccgcaaggac gccccgttcg
      121 acaaggcctg ctacatcggc tgcggcgtca ccaccggggt cggcgcggtg accaacaccg
      181 ccaaggtcga gcccggcgcc aactgcatcg tcttcggcct cggcggcatc ggcctgaacg
      241 tgatccaggg cctgaagctg gtcggcgcca acatgatcgt cggggtggac atcaatcccg
      301 accgcgaggc ctggggccgg cagttcggca tgacccactt cgtcaatccg aagaccgtcg
      361 acggcgacgt cgtcccgcac ctggtggccc tgaccgacgg cggggcggac tacaccttcg
      421 acgccaccgg caatgtcacg gtcatgcgcc aggcgctgga agcctgccat cgcggctggg
      481 gcgagagcat catcatcggc gtggccgagg ccggccgcga gatcgccacc cgcccgttcc
      541 agctggtcac cggccgggtc tggaagggca cggccttcgg cggcgcgaga ggccgcaccg
      601 acgtgccgcg gatcgtcgac tggtacatgg acggcaagat ccagatcgac ccgatgatca
      661 cccacaccat gccgctggaa gacatcaaca aggccttcga cctgatgcac gccggcgaga
      721 gcattcgaag cgtggtggtg ttctagggac ttttccagac ccgctcatcc cggcgaaggc
      781 cgggacccgg gctttttcag aagcacctcg gcggccgtac gaaacaacca aacctgggtc
      841 ccggccttcg ccgggatgag cggaagcgca catggtcgaa atcctctcca cccaccgcac
      901 gttcgaggga acgctgcact attgccgcca ccagagcgcg gcgaccggca cgccgatgcg
      961 gttcacgatc ttcctgccgc cgggcgaggg gccgtttccg gtgctggtct ggctgtcggg
     1021 cctgacctgc accgaggaga acttcaccac caagggcggg gcctacggcg cggcggcggc
     1081 gctgggcctg gccatcgtgg cgccggacac cagcccgcgc ggggacgagg tcgccgacca
     1141 cccggactac gacttcggca agggcgccgg cttctatatc gacgccacgg agaagccttg
     1201 ggcgccgcac ttcaccatgg agacctacat cgtccgcgac ctgctggagg ccgtgcacgg
     1261 cgccttcccg ctggacccgg cgcggcgggc gatcagcggc cattccatgg gcggccacgg
     1321 ggccctgacc ctggcgctga aacaccccga cctgttccgc tcggtcgggg ctttcgcgcc
     1381 gatctgctcg ccgacgcggt gtccctgggg gcggcgggcc ttcaccggct acctgggcgt
     1441 cgacgagacg gcctgggccg gccacgacgc caccctgctg atcgcggccg gcgccgcgaa
     1501 gggccgctac gacgacatcc tggtggagca ggggaccgcc gacccctggc tggcggaaca
     1561 gctgaagccc gaactcctgg aagccgcctg tgaggccgcc ggccagccgc tgaccgtcct
     1621 gcgccacgag ggctacgacc acggctactt cttcgtcgcg accttcatcg ccgaccatct
     1681 ggcctggcat cgcgagcggc tggagtaggt ccttcaggac gccggcggcg aatcctcggg
     1741 cgctgtgcgg gatgcctgcc gccgcatcag ccgctcgacc gtcgccccct ggacgaccac
     1801 cgagaacatc accaccagcc aggtcgccgc cagcagggcg tcgcgccacg ggcttggtgg
     1861 cagggacagc gccagcgcaa tggagatgcc gccgcgaagt ccaccccagg tcaggagggg
     1921 gaaggcttgg ccgaacggga gaatccggcg ccagatcagg agcggcccgg ccaccgacgc
     1981 cgcccgcgcg atcagcgtca ggggaatggc cgccagtccc agcagcgcca ccccggcatt
     2041 gccgagcaga accacgccct ccagaccgat gagcaggaac agggcggcgt tgaggatgtg
     2101 gtcgatcagg tcccagaact tgagcagatg gtcgcgggtg gtgtcgctca tcgccatggc
     2161 cacgccgtgg ttgccgatga ccagcccgcc aacggccatg gcgaccggcc cgctcacatg
     2221 cagcaagtgg gccagggagt agccgcccat gaccagggcc agggaaatca gcacctcgag
     2281 cacatagtcg tcgatcgacc gcatggccag gaacgccacc cagccgagga ccagcccgac
     2341 cgccgccccg ccgaaggcct cgacgacaaa catttccgcg cccttgagcg gggtaatttc
     2401 gaccccgccc acggcggcgg ccagaagtat gctgaacagc accaccccga cgccatcgtt
     2461 gaacaggctt tctccggcga ccgtggcctt gagcgtgcgc ggggcgtcgg accgggccag
     2521 cagggccatc accgccaccg gatcggtggg actgatcagg gcgccgaaca ccaggcacca
     2581 ggcgagggag atttccgccc ccagcaggcg cgacaacagg tgcaggccgg ccgccacgat
     2641 cacggtcgac agcagcagcc cgagactgga caggacggcg atcggccagc ggccgatctt
     2701 caattgcctg atgtcgacat gcagcgcccc ggcgaacagc aggaaggaca gcatgccgtc
     2761 gagcagggcg accttgaagt cgacctggtc gaggaaggtt cgcacgccat cgccaagctg
     2821 gccgccaggc agcaggaaat cgaccacggc cacgacgacc gaggccagag cacccatcag
     2881 cgacagggcg atggtctgcg gcagccggat gaaccgatga ttgacgtagc cgagggcggc
     2941 cgccgccacg atgacgatcg acgcggcctc gaacggcccg atgttcagag tcacggcgat
     3001 gtcctcctgc ggggaccctt atcgccgcca tggatggccc gcgccaaggg cgcgtag
//
LOCUS       DAORNX010000363         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24258_length_3052_cov_6.883550, whole genome shotgun sequence.
ACCESSION   DAORNX010000363 DAORNX010000000
VERSION     DAORNX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24258_length_3052_cov_6.883550"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLO65_13655"
     CDS             <1..749
                     /locus_tag="PLO65_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HRD29339.1"
                     /translation="AASARALGAWALIIEREDRILARVACPELSAFFQDYHQARGVNF
                     ELSAGVKAIEGENGQVRRVRLSDDRVVECDAVLVGVGALPNEEIARDAGLDCANGVVV
                     DLQARTSDPAVFAIGDVTHRPLPLYDRQFRLESVPNALEQAKQVASAITDHAPPNPEA
                     PWFWSDQYDLKLQIAGLPFDADSIVIRGDRAAAKFAIFHLKGDQVQAVEAVNAPPEFM
                     MGKMLILSRKPVSRERLADSSISMKEVAAG"
     gene            785..1105
                     /locus_tag="PLO65_13660"
     CDS             785..1105
                     /locus_tag="PLO65_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD29340.1"
                     /translation="MAKITYIEHDGTEHTVDVKTGLSVMEGAIKNNIPGIDADCGGAC
                     ACATCHVYVDDAWASKTGEKSAMEESMLDFAENVEPSSRLSCQIKVTDDLEGLVVRMP
                     ESQH"
     gene            complement(1240..>3052)
                     /locus_tag="PLO65_13665"
     CDS             complement(1240..>3052)
                     /locus_tag="PLO65_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRD29341.1"
                     /translation="ARPEPAWIAGADGAVQWANTAWLAAVSARDAGDAASLGLSLDKG
                     ADALVSEAANLGERRELTRWISLAGRRRALKITAEPLEGGGVAVWTTDATQAEEIQDA
                     LKRHVAAHDETLNHIAEAVAIFGPTRRLSFHNTAFAELWGLETAWLAERPTHGEILDR
                     LRQRQRLPETPDYARWKAAELARYETLGPQPDDRWPLPDGRTLRLVRQPHPMGGLLLL
                     YSDITGEVRLKAQYNALIQVQQATLDKLSDAVAVFGSNGRLRLHNEAFSRFWNVAEDE
                     LAEGVDFERVVELCVPRLHDMAFWRELKGRVGDPDPEARAPTAGEVRNSLGRIVSWQT
                     RPLPDGATLIAFADITDARQLEGALADKEAALDSAERLKREFVGNVSYELRTPLTTII
                     GYSELLDRMGEAIPERGRSHLSAVRQAAVQLGRSIDDVLDIAAIDAEEMALDMGDVRV
                     GDLLQETAARWAATAETAKVSLVMDVGDSDIGLIRGDSKRLVQTLDHLVENALRQTPP
                     GGTVTLSAVRAAGEVRLAVSDTGRGVPFHVQAHIFDRFVGRDRGGPGLGLALVKALVE
                     LHGGWVALESEPGHGSTFTCHLPEAAQVSAGQKELGF"
BASE COUNT          456 a         1062 c         1081 g          453 t
ORIGIN      
        1 cggcggcgtc ggcccgcgcg ctcggcgcct gggccctgat catcgagcgc gaggaccgta
       61 tcctggcccg cgtcgcctgc ccggagctgt cggccttctt tcaggactat catcaggccc
      121 ggggggtcaa cttcgagctg tccgccgggg tcaaggcgat cgagggcgag aacggccagg
      181 tccgccgggt gcggctgtcc gacgaccggg tggtcgagtg cgacgcggtg ctggtcggcg
      241 tcggcgccct gcccaacgag gagatcgccc gggacgcggg cctggactgc gccaacggcg
      301 tggtcgtcga cctgcaggcg cggaccagcg acccggcggt gttcgccatc ggcgacgtga
      361 cccaccggcc gctgccgctc tacgaccggc agttccggct ggagagcgtg cccaacgccc
      421 tggagcaggc caagcaggtc gccagcgcga tcaccgacca cgcgccgccc aatcccgaag
      481 cgccgtggtt ctggtccgac cagtatgacc tgaagctgca gatcgctggc ctgcccttcg
      541 acgcggactc gatcgtcatc cgcggcgacc gggccgccgc gaaattcgcc atcttccacc
      601 tgaagggcga ccaggttcag gcggttgagg cggtcaacgc cccgccggag ttcatgatgg
      661 gcaagatgct catcctgtcg cgcaagccgg tctcgcgaga gaggcttgcg gattcgtcca
      721 tttccatgaa agaggtcgcc gccggataag gcggggaaac agaagaagag gcgtcaggga
      781 gcgtatggcc aagatcacct acatcgaaca cgacggaacc gaacacacgg tcgacgtgaa
      841 gaccggcctg tccgtcatgg aaggggcgat caagaacaac atccccggca tcgacgctga
      901 ctgcggcggc gcctgcgcct gcgccacctg ccacgtctat gtcgacgacg cctgggcgtc
      961 gaagaccggc gagaagtcgg ccatggagga gtcgatgctc gacttcgccg agaacgtcga
     1021 accctcgagc cgcctgtcct gccagatcaa ggtcaccgac gacctcgagg gcctggtcgt
     1081 gcgcatgccg gaaagccagc actaggccct caccgcttcg cggttcgaga gggagtaggg
     1141 cttggggagt ggggagtaga tcgacctgcg cgcgtcgcag gcgccgcgac ccgactttgc
     1201 tactccctac tccctactcc ccaagcccta cgcccttctt caaaacccca gctccttctg
     1261 ccccgcggaa acctgcgcgg cctcgggcag gtggcaggtg aaggtcgagc cgtgaccggg
     1321 ttcgctttcc agcgccaccc agccgccgtg cagctcgacc agcgccttga ccagggccag
     1381 gccaaggccc ggcccgccgc ggtcgcgacc gacgaagcgg tcgaagatgt gcgcctggac
     1441 gtggaacggc acgccgcgtc cggtgtcgct gaccgccaac cgcacctcgc ccgccgcccg
     1501 caccgccgac agggtcaccg tgccgcccgg cggcgtctgg cgcagggcgt tctcgaccag
     1561 gtgatcgagg gtctggacca gccgcttgct gtcgccgcgg atcaggccga tgtcgctgtc
     1621 gcccacgtcc atcaccaggg acaccttcgc cgtctcggcc gtggcggccc agcgggcggc
     1681 ggtctcctgc agcaggtcgc cgacgcgcac gtcgcccatg tcgagggcca tttcctcggc
     1741 atcgatggcc gcgatgtcga gcacgtcgtc gatcgagcgg cccagttgca cggcggcctg
     1801 acggacggcc gacagatgtg agcggccgcg ttcggggatc gcctcgccca tccggtcgag
     1861 cagttccgaa tagccgatga tggtggtcag gggcgtgcgc agttcatagg agacgttgcc
     1921 gacgaactcg cgcttcagcc gctcggcgct gtcgagcgcg gcctccttgt cggccagcgc
     1981 cccctcgagc tggcgggcgt cggtgatgtc ggcgaaggcg atcagggtgg cgccgtcggg
     2041 cagggggcgg gtctgccagg acacgatgcg gccgagagag ttgcgaacct cgccggcggt
     2101 cggcgcgcgg gcttccgggt cggggtcgcc gacccgcccc ttgagttcgc gccagaaggc
     2161 catgtcgtgc agacggggga cgcacagctc gacgacccgc tcgaaatcga cgccctcggc
     2221 cagctcgtcc tcggcgacgt tccagaaccg ggagaacgcc tcgttgtgca gccgcagacg
     2281 gccgttggaa ccgaacacgg ccaccgcgtc gctgagcttg tcgagggtcg cctgctgcac
     2341 ctggatcagg gcgttgtact gcgccttgag gcggacctcg ccggtgatgt cggaataaag
     2401 cagcagcagg ccgcccatcg gatggggctg acggaccagc cgcagggtgc ggccgtccgg
     2461 caacggccag cggtcgtcgg gctgcggtcc gagggtttcg tagcgcgcca gttcggcggc
     2521 cttccaccgg gcgtagtccg gggtctcggg cagccgctgc cgctggcgca gacggtcgag
     2581 gatctcgcca tgggtcgggc gttcggccag ccacgccgtc tccagccccc acagctcggc
     2641 gaaggcggtg ttgtggaacg acagccgtcg ggtggggccg aagatggcca ccgcctcggc
     2701 gatatggttg agcgtctcgt cgtgcgccgc cacatggcgc ttgagggcgt cctggatctc
     2761 ctcggcctgg gtcgcgtccg tggtccagac cgcgacgccg ccgccttcca ggggctcggc
     2821 ggtgatcttc agcgcccgcc gccggccggc cagcgagatc cagcgggtca gctcccggcg
     2881 ctcaccgagg ttggccgcct cgctgaccag ggcgtcagcg cccttgtcga gggacaggcc
     2941 caggctggcc gcgtcgccgg cgtcgcgggc gctgaccgcg gccagccagg cggtgttggc
     3001 ccactgtacg gccccgtccg ctccggcgat ccaggccggc tcgggccggg cg
//
LOCUS       DAORNX010000364         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24270_length_3051_cov_6.251335, whole genome shotgun sequence.
ACCESSION   DAORNX010000364 DAORNX010000000
VERSION     DAORNX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24270_length_3051_cov_6.251335"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLO65_13670"
     CDS             complement(<1..572)
                     /locus_tag="PLO65_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD29342.1"
                     /translation="MSSSLESLLGQAAQLRRAGRTLEALAAYEQLLADHPDLPDSWYN
                     LALLQRQAGRPQAALASYRQALNRGISEPEEVHLNRAVILSDDLRDEVGAMAELKTAL
                     VLNPAYVPALLNLGNLHEDHGRRAEAVTAYNAILARHPDDPVALARIGGLRAPTGPDD
                     PLIGRLRVALARPGASVADRAALGFALGRLL"
     gene            complement(618..1124)
                     /locus_tag="PLO65_13675"
     CDS             complement(618..1124)
                     /locus_tag="PLO65_13675"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRD29343.1"
                     /translation="MIRFVAIAAIGALSAVGAARAVVSLDPARYAEGPRAELALQSAV
                     APAAAASIRKAADGHYWAEAQVDGKAVRFLVDTGATAVSLTLDDARRLGIDTANLSYA
                     YDVITADGKARAAAVRLASVSIAGARVENVDAFVIEKGLTASLLGMSYLGRLRAFEAT
                     QSALILKP"
     gene            complement(1243..1467)
                     /locus_tag="PLO65_13680"
     CDS             complement(1243..1467)
                     /locus_tag="PLO65_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRD29344.1"
                     /translation="MTAGPPKAVATPCVKVCVVDPESSLCLGCFRTLMEIAGWARLDD
                     GERERVMADLPSRRSRIRPEKLGLFPGPAR"
     gene            complement(1464..1922)
                     /locus_tag="PLO65_13685"
     CDS             complement(1464..1922)
                     /locus_tag="PLO65_13685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD29345.1"
                     /translation="MIQEPVTGKTGLRMDAAALNAFLRRAFPEADPEQMAQVTTVAPG
                     RVMLTMPTASRNLRPGGVISGPTLMSLADTAAYALVLAHIGEVAMAVTTSLTMHFLRP
                     ARPGVLHAEADLLKLGRRIATCEVSLWTEGRDRLAAKATVAYAIPEAAAQ"
     gene            2123..2425
                     /locus_tag="PLO65_13690"
     CDS             2123..2425
                     /locus_tag="PLO65_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29346.1"
                     /translation="MRILVIAATALSVGAASSAAFAADARLSDSQFVKAAHCRGLASG
                     EAAARFDALIKSQKRGRSDHIVDRAWNARTDAERLARTDASAAQAALASDCANLGA"
     gene            2690..2998
                     /locus_tag="PLO65_13695"
     CDS             2690..2998
                     /locus_tag="PLO65_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29347.1"
                     /translation="MRILTVAVILAAATATSAFAQERLTDTQYITAAKCRGLMQGADT
                     AGIDAVLKSNKAGRVGVVLDRADTARKAAASKARKASGPGGKAEVEREIATACGGFTS
                     "
BASE COUNT          483 a         1041 c         1066 g          461 t
ORIGIN      
        1 agcagccggc caagggcgaa gcccagcgcc gcgcggtcgg cgacggacgc gccaggccgg
       61 gccagggcga cccgcaggcg tccgatcaac gggtcgtccg gacccgttgg cgcgcgcagg
      121 ccgccgatcc gcgccagggc gaccggatcg tccggatgcc gtgcgaggat ggcgttgtag
      181 gcggtgacgg cctcggcccg ccggccatgg tcctcatgca ggttgccgag gttcagcagg
      241 gccgggacgt aggccggatt caggaccagg gccgtcttca gctcggccat ggccccgacc
      301 tcgtcccgca ggtcgtcgga caggatcaca gcgcggttga ggtgaacctc ttcgggctcg
      361 gaaatcccgc ggttcagcgc ctggcgatag gaggcgagcg ccgcctgcgg ccgcccggcc
      421 tgccgctgca gcagggccag gttgtaccag ctgtcgggca ggtcgggatg gtcggcaagg
      481 agctgctcat aggccgccag ggcctcgagc gtccgccccg cgcgacgcag ctgggcggcc
      541 tggccgagca gggattcgag gctggacgac atgaccccat tgaacggcct cgccgcggcc
      601 ggctcaaggg cgcgtggtca gggcttgagg atcagggcgc tctgggtggc ctcgaaggcg
      661 cgcaggcggc ccaggtagct catgccgagc agggaggcgg tcaggccctt ctcgatgacg
      721 aaggcgtcga cgttctcgac ccgggccccg gcgatcgaga cgctggcaag gcggaccgcc
      781 gcggcgcgcg ccttgccgtc ggcggtgatg acgtcatagg cgtagcttag gttggcggtg
      841 tcgatgccga gccgacgggc gtcgtcaagg gtcagggaga cggcggtggc cccggtgtcg
      901 accaggaacc ggaccgcctt gccgtcgacc tgggcctcgg cccagtagtg gccatcggcg
      961 gccttgcgga tcgaggccgc ggcggccggc gcgacggcgg actgcagggc cagctcggcg
     1021 cgcgggccct cggcgtaacg ggcagggtcg agactgacca ccgcgcgcgc ggcgccgacg
     1081 gccgacaggg cgccaatcgc cgcgatcgcc acgaacctga tcatcagaca ccccgccata
     1141 ttggcttagc cgagggcgtt ttttccgccc cttcatcgcc accctaactg gccggggttt
     1201 gcaatccgtg aagggcggtc agcgggccgc gcgcgggcgg cttcagcggg ccgggccggg
     1261 gaacaggccc agcttctcgg gccggatccg cgatcgccgg gacggcaggt cggccatcac
     1321 ccgttcgcgc tcgccgtcat cgagccgggc ccagccggcg atctccatca gggtgcggaa
     1381 gcagcccaga cacaggctgc tttccgggtc gacgacgcag accttcacgc agggcgtggc
     1441 gacggctttc ggcggcccgg cggtcattgc gcggccgcct ccggaatggc gtaggccacg
     1501 gtggccttgg ccgccagccg gtcgcggcct tcggtccaca gcgacacctc gcaggtggcg
     1561 atccgccggc cgagcttgag caggtcggcc tcggcatgga gcacgcccgg cctcgccggc
     1621 cgcaggaaat gcatggtcag ggaggtggtc acggccatgg ccacctcgcc gatgtgggcc
     1681 agcaccaggg cgtaggcggc cgtatcggcc aggctcatca gtgtcgggcc tgagatcaca
     1741 ccgccgggtc gcaggttgcg gctggcggtg ggcatggtca gcatgacgcg gccgggggcc
     1801 accgtcgtga cctgcgccat ctgttcgggg tcggcctcgg ggaaggcgcg gcggaggaag
     1861 gcgttgagcg cggcggcgtc catgcgcagc ccggtcttgc cggtaaccgg ttcctggatc
     1921 atcgccgcac catcgccgtc gcggagccgt gattcaagga gtcattgtcg ccgaaaagac
     1981 gtcatgattt ggccatcttc agtgcggtcg gactcaacat gaatagtctg taattattca
     2041 aacatttcag gtatttaatt tgtgaataca tgacgggaaa agcatcctga tcctgcatcc
     2101 aatcagggag atggagcgac ccatgcgaat cctcgtcatc gccgccacgg ctctttccgt
     2161 cggcgccgcc tcgtccgccg cctttgccgc cgacgctcgc ctgagcgaca gccagttcgt
     2221 caaggccgcc cattgccggg gcctggccag cggcgaggcg gcggcccggt tcgacgcgct
     2281 gatcaagagc cagaagcgtg gccggtccga ccacatcgtc gatcgagcct ggaacgcccg
     2341 caccgatgcc gaacggctgg cgcgcaccga cgcttccgcc gcccaggcgg ccctcgccag
     2401 cgattgcgcc aacctcggag cctgagtttt cgggcccctc accgcccgaa catccagagc
     2461 cccttgcgcc ccgtctcccc cgacggggcg cctttttttt gggtcaacat caagaacgtc
     2521 ggtattcatc cggttggatg cagggaaaga aattaaattt tgtccacagt tcacgaaatg
     2581 ggcggattaa atcgccgtaa ttactgcttt atttcagaca tgtaatttgc gaatacattt
     2641 ggctgggcgc attcattggc tcgtccacca agggaaaggg agcctcccga tgcgtatcct
     2701 caccgtcgcc gtcatcctgg ccgccgcgac cgccaccagc gccttcgccc aggagcgtct
     2761 caccgacacc cagtacatca ccgccgccaa gtgccgcggc ctgatgcagg gcgccgacac
     2821 cgccggcatc gacgccgtcc tcaagtccaa caaggccggg cgcgtcggcg tcgtgctcga
     2881 ccgcgccgac accgcccgca aggccgcggc cagcaaggcc cgcaaggcca gcggtccggg
     2941 cggcaaggcc gaggtggagc gcgagatcgc caccgcctgc ggcggcttca ccagctgagt
     3001 ccggcccgcg ccccggcgcg ggtgatcacc gatccagtcg agcaccctgg a
//
LOCUS       DAORNX010000365         3046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24332_length_3046_cov_2.880976, whole genome shotgun sequence.
ACCESSION   DAORNX010000365 DAORNX010000000
VERSION     DAORNX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24332_length_3046_cov_2.880976"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1126)
                     /gene="dusA"
                     /locus_tag="PLO65_13700"
     CDS             complement(113..1126)
                     /gene="dusA"
                     /locus_tag="PLO65_13700"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRD29348.1"
                     /translation="MCNGKVTQFQAHRLSIAPMMDWTDRNCRAFHRVLTRRALLYTEM
                     VTTGAVLHGDRDRLLGFDSCEHPVALQLGGSDPADLAAAARIGEDLGYDEINLNVGCP
                     SDRVQSGRFGACLMREPELVADGMAAMKAAVSVPVTVKCRIGVDDQDPEESLFRLVDL
                     SAAAGVTTFVVHARKAWLQGLSPRENRDIPPLDYPLVYRLKRERPALTIVINGGVASL
                     DEALAHLEQVDGVMLGRAAYHEPGILGAVDARIFGEGETVDPFAAVERYRPYVARRLG
                     QGFHLAGMTRHMLGLFHGRPGARAWRRILTVEGSRAGAGLEVLDAALAAVSAAAARKA
                     EAA"
     gene            1093..2289
                     /locus_tag="PLO65_13705"
     CDS             1093..2289
                     /locus_tag="PLO65_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD29349.1"
                     /translation="MLEIASLFHYKSSGSALTGVALPTLFFDRFRHVSTSHCTGFAPK
                     MASIRKQKSGTWRVQVRRKGRTVSETFVRYDDAKKWGVDAERQIDRGETPTLSKVGRL
                     QTFGDLIDLHIADMTDVGKPPGRSKDATLAMLKRRLGARNMVELDRERLLTFGRERAA
                     EGAGPMTLSIDIGMIKLILSHAAAVHGLPVRVEPVDLARIALKRLGLVGKGKERDRRP
                     SDEELEKLMAHFDGNPRQIIPMSRIIKFAVATAMRQEEICRVTWSDLNERTKMLTIRD
                     RKDPREKKGNDQRIPLLSVSGFDALALIEEQRAIRGNEDDRIFPYAHKSAGTAFTRAC
                     QDLKIDDLHFHDLRHEGTSRLFEAGFEIQQVALVTGHKDWKILRRYTHLRPEALHAIA
                     DKKAAA"
     assembly_gap    2349..2392
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2452..>2797
                     /locus_tag="PLO65_13710"
     CDS             2452..>2797
                     /locus_tag="PLO65_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29350.1"
                     /translation="MTPWEHHSDLQRDRLIEVGRLIHQGRNDALDRQDANVGSIPWTV
                     GCEAFAFGMHQIAAAAADLDWLEVLQPGMQFVFSIGDVPVRFYKGAPDEPNKRTLRQT
                     FSELNQLSLFGAD"
     assembly_gap    2798..2808
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2809..>3046
                     /locus_tag="PLO65_13715"
     CDS             <2809..>3046
                     /locus_tag="PLO65_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29351.1"
                     /translation="STERVYRFAVETDFDGSVAAISFVVLDGETPVLTWNVPLDEPVV
                     RIAPLDIPDAKGVDLPKPLVIAPGLDHKKGESGGE"
BASE COUNT          541 a         1004 c          951 g          495 t
ORIGIN      
        1 gacgaccgac cggagcgcat cattccatca aggccaaagg gccgatgtca tagggccgga
       61 tatcagagac gggcgcggtc cctccgcgcg cgccctgtcc cttcgggcga cgtcaggcgg
      121 cctcggcctt ccgcgccgcc gccgcggaca ccgccgccag ggccgcgtcc agcacctcca
      181 gcccggcccc ggcccggctc ccctcgaccg tcaggatccg ccgccaggcg cgggccccgg
      241 gacggccatg gaacaggccc agcatgtgcc gggtcatgcc ggccagatgg aagccctgtc
      301 ccagccgcct ggcgacgtag gggcggtagc gctcgaccgc cgcgaacgga tcgaccgtct
      361 cgccctcgcc gaagatccgg gcgtcgaccg ccccgagaat gccgggctcg tggtaggccg
      421 cccggcccag catcaccccg tcgacctgct ccagatgggc cagggcctcg tccagcgacg
      481 ccacgccgcc gttgatcacg atggtcaacg ccggccgctc gcgcttgagc cggtagacca
      541 gcgggtagtc gagcggcggg atgtccctgt tttcccgggg cgaaagaccc tgcagccagg
      601 ccttgcgggc atggacgacg aaggtcgtca ccccggccgc ggccgacagg tcgacgaggc
      661 ggaacaggct ctcctccggg tcctggtcgt cgacgccgat gcggcatttg acggtcaccg
      721 gcacggacac ggcggccttc atggcggcca tgccgtcggc gaccagctcc ggctcgcgca
      781 tcaggcaggc gccgaagcgg ccgctctgca cccggtcgga ggggcagccg acattgaggt
      841 tgatctcgtc gtagccgagg tcctcgccga tgcgggcggc ggcggccagg tccgccgggt
      901 ccgacccgcc cagctgcagc gccaccggat gctcgcagga atcaaacccc agcagccggt
      961 cccggtcgcc atgcagcacc gccccggtcg tgaccatctc cgtatagagc agcgcccgcc
     1021 gcgtcaggac ccggtggaac gcccggcagt tccggtcggt ccagtccatc atgggtgcaa
     1081 tggacaatct atgtgcttga aattgcgtca cttttccatt acaaatcaag tggttcggcg
     1141 ctgaccggcg tcgcccttcc tacgctattt ttcgaccgtt ttcgccacgt ttcgacatct
     1201 cactgcaccg gatttgcacc caaaatggca tcgatccgaa aacagaaatc cggaacctgg
     1261 cgggtccagg ttcgccgcaa gggacggacc gtcagcgaga cgttcgtccg ttacgacgac
     1321 gccaagaaat ggggcgtcga cgccgagcgc caaatcgacc gcggcgagac ccccactctc
     1381 tccaaggtcg ggaggctaca gactttcggc gatctgatcg atctgcacat cgccgacatg
     1441 acggatgtcg gcaagccgcc cggacgttcc aaggacgcca cgctcgccat gttgaagcgc
     1501 cggcttggcg cccgtaacat ggtggaactg gaccgcgagc gccttctgac ctttggccgt
     1561 gaacgggcgg cagagggtgc cgggccgatg acgctcagca tcgacatcgg catgatcaag
     1621 ctgatcctct cgcacgcggc ggccgtccac ggcctgcccg tgcgggtgga gccggtcgat
     1681 ctggctcgca tcgccctcaa gcgcctgggt ctggttggta agggcaaaga gcgggaccga
     1741 cggccgagcg acgaagagct tgagaaactc atggcccatt tcgacggaaa tccccgtcag
     1801 atcatcccca tgagccgcat catcaagttc gcggtcgcca ccgcaatgcg ccaggaggaa
     1861 atctgccggg tcacctggag cgacttgaat gagcgcacca agatgctgac gatccgcgac
     1921 cgtaaggacc cgcgggagaa gaagggcaac gaccagcgca ttcccctcct ctcggtgtcc
     1981 ggcttcgatg cgctagcgct gatcgaagag cagcgcgcca tccggggcaa cgaggacgac
     2041 cgcatcttcc cctacgccca caaatccgcc gggacagcgt tcacgcgagc ttgccaggac
     2101 ctgaagatcg atgacctgca tttccacgac ctgcgccacg agggcacgag tcgccttttc
     2161 gaggcagggt tcgagattca gcaggtcgcc ctcgtcaccg gccacaagga ctggaagata
     2221 ctgcgacgct acacgcacct tcgtccagaa gcgctccacg cgatagcgga caagaaggcc
     2281 gctgcctaat ctgcgcaact gagtgggcgg ttaccgctag cggcgggcgt tttggccgcc
     2341 cgtcgcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagtcggt
     2401 tgacattgtc gcgaatacgc ccattctcgg cttgccaatc ggagcaaccg catgacgcct
     2461 tgggaacatc actctgacct tcagcgtgat cgcctgattg aggttggccg cttgattcat
     2521 cagggccgca atgacgcgct cgatcgtcag gatgccaatg tcgggtcgat cccttggaca
     2581 gtgggctgtg aagccttcgc tttcgggatg catcagatcg cagcggcggc ggcggatctc
     2641 gattggctcg aggtacttca accggggatg cagtttgtct tcagcatcgg ggatgtcccg
     2701 gtgcggttct acaagggcgc tccggacgaa ccgaacaagc ggacgctccg tcagaccttt
     2761 tccgagctga accagttgag cctgttcggc gcggacgnnn nnnnnnnnct caacagagcg
     2821 agtttaccga ttcgcggttg agaccgactt tgacggctcg gtcgcggcta tatcgttcgt
     2881 cgtgctcgac ggggaaaccc cggtgctgac gtggaacgtg ccgctggatg agccggtcgt
     2941 tcggattgcg cccctcgata tccccgacgc gaaaggcgtt gatctgccca aacccttggt
     3001 tattgctccc gggcttgacc acaagaaggg cgagagcggc ggcgag
//
LOCUS       DAORNX010000366         3033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24462_length_3033_cov_6.388852, whole genome shotgun sequence.
ACCESSION   DAORNX010000366 DAORNX010000000
VERSION     DAORNX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24462_length_3033_cov_6.388852"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLO65_13720"
     CDS             <1..403
                     /locus_tag="PLO65_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HRD29352.1"
                     /translation="AARLAALADRRRLVWVVGNHDADGPRRLPGEILDEMILGGLLLR
                     HEPQAGPQPREVSGHLHPAARIAGRGGRVRRRCFITDGGRVVLPAFGAYAGGLNVRDE
                     AFAGLFAGQPLAATLGEARVHAIGWASLVGD"
     gene            complement(609..1070)
                     /locus_tag="PLO65_13725"
     CDS             complement(609..1070)
                     /locus_tag="PLO65_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HRD29353.1"
                     /translation="MRQSSTRVPASAWTLGVVSLAPFYATAAIYCYGPPRFSAVALLA
                     LGAWSTAMLAYLGGIRAGLELGSDHPPRWSSLSLSLLTPIAGFGLLYGALVHRFDPVW
                     EVSGFLTAFLLVWLWDVRDIEGPAWRPRYRTLITAGAAIALAFALEQAMSM"
     gene            complement(1076..1840)
                     /locus_tag="PLO65_13730"
     CDS             complement(1076..1840)
                     /locus_tag="PLO65_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HRD29354.1"
                     /translation="MAADVTPSVPPASVSATLTETRVRVTSGFRGARIAVYGAVFDPA
                     GDTSDVVVVVRGPDQPMRIARKVHVAGLWLNSRPVVFEGAPGFYRAASTRPLNDIARF
                     GQLRKLGLGLDHLAIAAPAERRVETRYGVRDMVVSALGADYYAWRNAVVRLKQSTGLY
                     SADASGVTFVDSGLFRAEVDLPAEAPIGRYTARVILFQAGRPVSDRTLPLTVEKVGME
                     RALYLFAHQRPWTYGLVSALIALCAGWAASRAFRRS"
     gene            complement(1840..2760)
                     /locus_tag="PLO65_13735"
     CDS             complement(1840..2760)
                     /locus_tag="PLO65_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD29355.1"
                     /translation="MDLYLPIAEMSVNAPLLIALGAVVGFISGLFGIGGGFLMTPVLV
                     FMGIPPAVAVASLSSHVAASSFSSVTAYSRRRMVDFRMGGILAGGGVVGAFAGVEAFR
                     VLRLLGQADLVVAVSYLLFLGVIGGLMLWESLGTILRRRRGEPPPPRRDRRPILLYAL
                     PWKMRFPKSRLYISVIPPIVLGVFVGVLSAIMGVGGGFVLVPAMVYLLRMPASVVVGT
                     SLFQIVITTVLTSVLQAGRNQTVDIVLATLLLVGGVLGAQLGARASARFRAEELRALL
                     ALVVLLVGLRMGLGLFIRPDDPFVLVSGGG"
     gene            complement(2826..>3033)
                     /locus_tag="PLO65_13740"
     CDS             complement(2826..>3033)
                     /locus_tag="PLO65_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRD29356.1"
                     /translation="QASVGGGAGDVASAQRALSTLGYYRGPQDGVPSQGLKGAIAAFQ
                     RDQGIPASGALDAQTIQKLSTYAR"
BASE COUNT          515 a         1082 c         1088 g          348 t
ORIGIN      
        1 cgcggcgcgg ctggcggccc tggccgaccg ccggcggctg gtctgggtgg tcggcaacca
       61 cgacgctgat ggtccgcgca ggctgcccgg agagatcctc gacgagatga tcctgggcgg
      121 cctgctgctc aggcatgagc cccaggcggg gccgcagccg agggaggtgt cgggccacct
      181 tcatccggcg gccaggatcg cggggcgcgg cggacgggtg cggcggcgct gcttcatcac
      241 cgacggcggc cgggtcgtgc tgccggcctt cggcgcctat gccggcgggc tgaacgtcag
      301 ggacgaagct ttcgcgggtc tgttcgcggg ccagcccctg gccgcgacgc tcggcgaagc
      361 cagggtgcac gcgataggct gggcgtcgct ggtgggggat tagggccaat cgacattcag
      421 cgatcaaggg gccatcaaac cccgagtgtc cccgcgaagg cggggaccca agccattgtg
      481 gatgcttcac cggcgcatcg agccttgtaa caccccttgg gtccccgcct tcgcggggac
      541 aatcggtaac aaggcggccc cagcccctga atgtcgattc agcccaggcg ggactggagg
      601 ggaccggcct acatgctcat cgcctgttcc agcgcgaagg ccagggcgat ggcggcgccg
      661 gcggtgatca gggtgcggta gcgcggacgc caggcggggc cctcgatgtc ccggacatcc
      721 cacagccaga ccagcaggaa ggcggtcagg aagcccgaca cctcccagac cgggtcgaac
      781 cggtggacca gggcgccgta cagcaggccg aagccggcga tcggggtcag caacgacagc
      841 gacaggctcg accagcgcgg cgggtggtcg ctgcccagct ccaggcccgc ccggatgccg
      901 ccgagataag ccagcatggc ggtcgaccag gccccgagcg cgagcagggc gacggcgctg
      961 aacctgggag gcccgtagca gtagatcgcc gcggtggcat agaagggcgc caggctgacg
     1021 accccgagcg tccaggccga cgcgggaacc cgcgtgctcg actgcctcat ccccctcaac
     1081 tcctgcgaaa cgcccgcgag gccgcccagc cggcgcatag tgcgatcagc gccgagacca
     1141 gaccatacgt ccatggccgc tgatgcgcga agaggtaaag cgcgcgctcc atgccgacct
     1201 tctcgacggt cagcggcagg gtgcggtccg ataccggccg gcccgcctgg aacaggatga
     1261 cccgggccgt gtagcggccg atcggcgcct cggccggcaa atcgacctcg gcccggaaca
     1321 acccgctgtc gacgaaggtg acgccgctgg cgtcggcgct gtagaggccg gtcgactgct
     1381 tgagccgcac gaccgcattg cgccaggcgt aatagtcggc gcccagggcg ctgacgacca
     1441 tgtcgcgcac gccgtagcgc gtctcgacgc ggcgctcggc cggggcggcg atggccagat
     1501 ggtcgaggcc caggcccagc ttccgcagct ggccgaaacg ggcgatatcg ttcagcggcc
     1561 gggtcgaggc ggcgcgatag aagcccggcg cgccctcgaa caccaccggc cggctgttga
     1621 gccagagtcc ggcgacgtga accttgcggg cgatgcgcat cggctggtcc ggcccgcgca
     1681 ccaccaccac cacgtcgctg gtgtcgccgg cgggatcgaa caccgccccg tagaccgcga
     1741 tccgggcccc gcgaaagccc gacgtgaccc gcacccgtgt ctctgtcagg gtcgccgaga
     1801 ccgaggccgg cgggaccgac ggcgtgacat cggcggccat cacccgcccc ccgacaccag
     1861 cacgaacggg tcgtcgggcc ggatgaacag gccaaggccc atgcgcaggc cgaccagcag
     1921 caccaccagc gccagcaggg cccggagctc ctcggcccgg aagcgcgccg aggcccgggc
     1981 gcccagctgc gcgcccagca ccccgccgac cagcagcagg gtggccagca cgatgtcgac
     2041 cgtctggttg cggccggcct gcagcacgct ggtcaggacc gtggtgatga cgatctggaa
     2101 caggctggtg ccgaccacca cgctggcggg catgcgcagc aggtagacca tcgccggcac
     2161 caggacgaag ccgccgccca cgcccatgat ggccgacagc accccgacga acacgcccag
     2221 cacgatcggc ggaatgacgc tgatgtacag ccgcgacttg ggaaagcgca tcttccacgg
     2281 cagggcgtag agcaggatcg gccggcgatc ccggcgcggc ggcggcggtt ccccgcggcg
     2341 acggcgcagg atggttccca gcgactccca cagcatcagg ccgccgatca cgccgaggaa
     2401 cagcaggtag gagaccgcca ccaccaggtc ggcctgaccc agcagtcgca ggacgcggaa
     2461 ggcttcgacc cccgcgaagg cgcccaccac cccgccgccg gcgaggatgc cgcccatgcg
     2521 gaaatcgacc atccgccggc ggctgtaggc cgtgacgctg gagaaggacg aggcggccac
     2581 gtggctggac aggctggcca ccgcgaccgc cggcggaatg cccatgaaca ccagcaccgg
     2641 cgtcatgaga aatccgccgc cgatgccgaa caggccggaa atgaagccga ccacggcgcc
     2701 cagcgcgatc agcagcggcg cgttgacgct catctcggcg atgggaaggt agaggtccac
     2761 gtcctaccta tgcctttcgg ccgggcggtt gtagccggaa acccccgccc ggcgccgcgc
     2821 ccggatcacc gggcgtaggt cgacagcttc tggatcgtct gggcgtcgag cgcgccgctg
     2881 gccggaatgc cctggtcgcg ctggaaggcg gcgatggcgc ccttcaggcc ctgggaggga
     2941 acgccatcct gcggtccgcg gtagtagccg agcgtcgaga gcgcccgctg cgccgaggcg
     3001 acgtcgcccg ccccgccgcc gaccgacgcc tgg
//
LOCUS       DAORNX010000367         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_24889_length_2993_cov_6.258679, whole genome shotgun sequence.
ACCESSION   DAORNX010000367 DAORNX010000000
VERSION     DAORNX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24889_length_2993_cov_6.258679"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLO65_13745"
     CDS             complement(<1..1023)
                     /locus_tag="PLO65_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRD29357.1"
                     /translation="MTASAPSKPPARGRGARSNATSRFVSARREDFDDGWTPDDPQPV
                     QLKTVVLPDKARTIITRNASPDIHFDRSINPYRGCEHGCIYCFARPNHAYLGLSPGLD
                     FETRLFFKPEAARLLTAELSRKTYAPRSIQIGADTDPYQPLERKLRVTRQVLEVLQRF
                     NHPLTIITKSNLITRDIDILGEMGRAGLARAAVSITTLDRKLARSMEPRAATPQRRLD
                     AVRQLTAAGCPVTVMFAPVIPGLNDPELEQVLEASAEAGATAAGYVMVRLPLEITDLV
                     QEWLRTDYPDRADRVMSLVRQMRRGKTYTSEFGVRGRGEGPLADLVAARFRAARKRYG
                     LDRPREP"
     gene            1144..1590
                     /locus_tag="PLO65_13750"
     CDS             1144..1590
                     /locus_tag="PLO65_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD29358.1"
                     /translation="MSDRIEKSIDLKASVARVWRALTDSAEFGAWFRVDLDGPFVAGE
                     EVRGRVTHPGYEHLVWKATVVRMDEPTLFAFTWHPYAVDADVDYDSEPPTLVEFRLEA
                     LADGVRLTICESGFDALPAERRDEAFLRNSGGWTAQVENIRAHVER"
     gene            1577..1909
                     /locus_tag="PLO65_13755"
     CDS             1577..1909
                     /locus_tag="PLO65_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD29359.1"
                     /translation="MLNAERPAVGPARVFAALGDPTRLTLLRRLSGGAPLSIARLSQD
                     TRLTRQAVTKHLHVLESAGLVASLRVGRESRFSYRPEPVVEADAYLRSVSAQWDQALA
                     RLKVLVED"
     gene            1940..2374
                     /locus_tag="PLO65_13760"
     CDS             1940..2374
                     /locus_tag="PLO65_13760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD29360.1"
                     /translation="MISVIVQTLNNAATLPACLGALVPAAVDAIVTEVIVVDGGSTDA
                     TLEIIEDAGAKRADSLAAAVAAAKSDWLLLLPADLRLEAGWDARAAAHVQTSGGRGAR
                     FPSAPWWPPRLGDRRAVLTRKDRFGKPLGRLRRLDSRACPIS"
     gene            complement(2402..>2993)
                     /locus_tag="PLO65_13765"
     CDS             complement(2402..>2993)
                     /locus_tag="PLO65_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRD29361.1"
                     /translation="APAPAARRPAAPRRYRVETYPAPELWPEGGFTLDKALVYALVWK
                     ESGFNPFAVSGAGAMGLMQVRPVAAARAAGDDKLLVNPLPLLDAPTNLRVGQDYFTWL
                     MERALGGGPEAYDLLRAVAAYNAGAGTVLNTLKKIGPGADSLLVMESLPALETRDYVE
                     KVSVAYWTYRRRFGGETRTLDAVARGDAIIDIRLDR"
BASE COUNT          422 a          992 c         1110 g          469 t
ORIGIN      
        1 cggctcgcgc ggccggtcca ggccgtagcg cttgcgggcg gccctgaagc gggctgcgac
       61 gaggtcggcc agcggccctt cgccccggcc gcggacgccg aattcggacg tgtaggtctt
      121 gccccgccgc atctggcgga ccagcgacat gaccctgtcg gcccggtcag gatagtcggt
      181 cctgagccat tcctggacga ggtcggtgat ctccagcggc agccgcacca tgacatagcc
      241 cgccgcggtc gcgccggctt cggcggaggc ctccagcacc tgttcgagtt caggatcgtt
      301 gaggccgggg atgaccggcg cgaacatcac cgtcaccggg cagccggcgg ctgtcagttg
      361 ccgcacggcg tccagtcgtc gctgcggcgt ggcggcgcgc ggctccatgc tacgggccag
      421 cttgcggtcg agggtggtga tcgagacagc cgcccgggcc agtcccgccc ggcccatttc
      481 gccgaggatg tcgatgtcgc gggtgatcag gttggacttg gtgatgatcg tcagggggtg
      541 gttgaaccgc tgcagcacct ccagcacctg ccgggtgacg cgcagcttcc gctccagcgg
      601 ctgatagggg tcggtgtcgg cgccgatctg gatggaccgg ggcgcatagg tcttgcgcga
      661 cagttcggcg gtcagcagcc gcgcggcctc gggcttgaag aacagccgcg tctcgaaatc
      721 gagcccgggc gacaggccca ggtaggcgtg gttggggcgt gcgaagcagt agatgcagcc
      781 gtgctcgcag ccgcgatagg ggttgatcga gcggtcgaaa tggatgtccg ggctggcgtt
      841 gcgggtgatg atggtcctgg ccttgtcggg cagaaccacc gtcttcagct ggaccggctg
      901 gggatcatcg ggggtccagc cgtcatcgaa gtcctcgcgc ctggccgaga cgaagcggct
      961 ggtggcgttg ctgcgcgcgc cacggccgcg cgcgggcggc ttcgagggag cggatgcggt
     1021 catccggtga ggctacgcgc ccggatgaaa caaaacaaga acattgaggc cggcaattcg
     1081 caaccattgg gttgcggtat taccgcagcc acgctacgga aggacaacca ggaggttgct
     1141 gaaatgtccg accgtatcga aaagtccatc gacctgaagg cgtcggtcgc ccgggtctgg
     1201 cgcgccctga ccgactcggc cgagttcggc gcctggttcc gcgtcgatct ggacggtccg
     1261 ttcgtggcgg gcgaggaagt ccgcggccgt gtcacccatc ccggctacga gcatcttgtc
     1321 tggaaggcca ccgtggtgcg gatggacgag ccgaccctgt ttgccttcac ctggcatccc
     1381 tacgccgtgg atgccgacgt cgactatgac tccgagccgc cgaccctggt cgagttccgg
     1441 ctggaagctc tggccgacgg cgtgcgactg acgatctgcg agtccgggtt cgacgccctg
     1501 ccggccgaac ggcgcgacga ggccttcctg cgcaattccg gcggctggac cgcccaggtg
     1561 gagaacatcc gggcccatgt tgaacgctga gcgccccgcc gtcggtccgg cgcgggtctt
     1621 cgcggcgctc ggtgatccga cacggctgac gttgctgcgc cggctgagcg gcggagcccc
     1681 gttgtcgatc gcccggctgt cgcaggacac ccgtctgacc cggcaggcgg tgaccaagca
     1741 cctgcatgtg ctggaaagcg cgggcctggt cgccagcctg agggtcggcc gtgagagtcg
     1801 cttcagctac cggcccgagc cggtagtcga ggccgacgcc tacctgcgat cggtctcggc
     1861 gcagtgggat caggcgctgg cgcggctgaa ggtcttggtc gaggattgac ccgggcgggg
     1921 acgcgggctc catagcccga tgatctccgt catcgtccag accctgaaca acgccgcgac
     1981 cctgccggcc tgcctgggcg ccctggtgcc ggcggcggtc gacgcgatcg tcaccgaggt
     2041 gatcgtcgtc gatggcggct cgacggacgc gacgcttgag atcatcgagg atgccggcgc
     2101 caaacgggcg gacagcctgg ccgcggccgt ggcggcggct aagagcgact ggctgctgct
     2161 gctgcccgcg gatctgcggc tggaggccgg ctgggatgcg cgggcggccg cgcatgtcca
     2221 gacgtcgggc gggcgagggg cccggttccc ttcggcgcca tggtggccgc cgcgcctcgg
     2281 cgaccggcgg gcggtgctga cccgcaagga ccggttcggc aagccactgg gccggcttcg
     2341 ccggctggac agcagggcct gtccgatttc ctgagctctc ggacgggaga aggcgaaggc
     2401 cttaccggtc cagccggatg tcgatgatcg catcgccccg ggcgaccgcg tcgagggtgc
     2461 gggtctcgcc gccgaagcgg cgcctgtagg tccagtaggc gacggagacc ttctcgacgt
     2521 agtcgcgggt ctccagcgcc ggcaggctct ccatcaccag caggctgtcg gcccccgggc
     2581 cgatcttctt gagcgtgttg agcacggtgc cggccccggc gttgtaggcc gcgaccgccc
     2641 gcagcaggtc gtaggcctct ggcccgccgc cgagggcccg ttccatcagc caggtgaagt
     2701 agtcctggcc gacccgcagg ttggtcgggg cgtccagcag gggcagggga ttgaccagca
     2761 gcttgtcgtc accggccgcg cgggcggcgg ccaccggccg cacctgcatc aggcccatgg
     2821 cgcccgcgcc ggacacggcg aacgggttga acccgctttc cttccacacc agggcgtaga
     2881 ccagcgcctt gtcgagcgtg aagccgccct cgggccacag ctccggcgcc ggataggtct
     2941 cgacccggta gcggcgcggc gcggccggac gccgggctgc cggcgccggc gcg
//
LOCUS       DAORNX010000368         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25074_length_2976_cov_9.731941, whole genome shotgun sequence.
ACCESSION   DAORNX010000368 DAORNX010000000
VERSION     DAORNX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25074_length_2976_cov_9.731941"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLO65_13770"
     CDS             <1..517
                     /locus_tag="PLO65_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HRD29362.1"
                     /translation="AARAAVQQARLDLERTVIRAPAAGVVGARSVRPGQLVQPGVTLM
                     SVVPLGKAFIVANFKETQLARLRIGQPVEIKADAFGGQTIKGRVQSFAPATGAEFALI
                     PVENAVGNFTKIAQRIPVRIAVEEGESLASALRPGLSVEVKVDVRGRTGQSFAESGQA
                     PAQYARTGESR"
     gene            522..2135
                     /locus_tag="PLO65_13775"
     CDS             522..2135
                     /locus_tag="PLO65_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRD29363.1"
                     /translation="MTAAVLTGPAAPPARETAEPQVNWLMLFLGFGGMAIGQFMAILD
                     IQIVAASLPQIQAGIEASADQISWIQTAYLIPEVVMIPLSAYLARLWGTQRVFMASCG
                     GFLIMSVLAGCSTNIDMMIVCRALQGFVGGAMVPTVFATAFSAFPPERRVTANVVTGM
                     IVTLAPTVGPTLGGHLTDWLNWRWLFFINVVPGLLSLFLVGRYGNFDKGDRRLAQNFD
                     WFGLAMMAIFLMSMQYVVEEGAKDNWFQDTAILWLTVLASVTGVIFVWRQLTYFQPIV
                     QLKAIADRNFALGLVMGATSGLALYGGTFVLPIYLGRIRGFSASEVGTTMLVSGISMF
                     ITGPLAGRWVRAVDPRIPIVIGYGMVAWGMWLGHDINGEWGFAEFAALQAFRGVGVMV
                     AMIASTQMTMSTLPLHLIKDASGLLNLARNTAGAIGMAIIASTLTSQGAVHMNDLVSR
                     MDRSSIEGQAMLAGLTARFQQLGVSDPEGAAYKAMHYMISQKAQILAFGDVFAFMAVC
                     AMIAALLSILAKPGKVPPGGRALTPIEDH"
     gene            2137..2742
                     /locus_tag="PLO65_13780"
     CDS             2137..2742
                     /locus_tag="PLO65_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29364.1"
                     /translation="MPRLVGQIDAVKSEAILDAASEVLGERGLSAPMEEIARRAGVSK
                     QTVYNHYGCKADLVRALVERRVNSITAPLREAGAEDRPEETLTAYARTLLSVVNNRSY
                     ALMRVTIQGAGEMPELAREVFEAGPKHSRAQLARFLEVETLAGRLRIGDPMQAAEFFG
                     GMVLGHRQTKALLNIAPDLTEHEVEAIAAEAVRVFMRAYAP"
     gene            complement(2759..>2976)
                     /locus_tag="PLO65_13785"
     CDS             complement(2759..>2976)
                     /locus_tag="PLO65_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29365.1"
                     /translation="TLSLAGISGGRGMISQGRAFSALKEDTSMEGVAGAALWLLSDLG
                     RSTTGEVVHVDAGFHMIGLPDGDLAEG"
BASE COUNT          444 a         1061 c          990 g          481 t
ORIGIN      
        1 cgccgcccgc gccgccgtgc agcaggcccg tctcgacctg gaacgcaccg tgatcagggc
       61 cccggccgcc ggcgtggtcg gcgcccggtc ggtccgtccc ggccagctgg tccagcccgg
      121 ggtgacgctg atgagcgtgg tgcccctggg caaggccttc atcgtggcca atttcaagga
      181 aacccagctg gcccgcctgc gcatcggcca gccggtcgaa atcaaggccg acgccttcgg
      241 cggccagacc atcaagggtc gggtgcagag cttcgccccg gccaccggcg ccgagttcgc
      301 cctgatcccg gtggagaacg ccgtgggcaa cttcaccaag atcgcccagc ggattccggt
      361 gcggatcgcc gtcgaggagg gcgagtccct ggcctcggcc ctgcgccccg gcctgtcggt
      421 ggaggtcaag gtcgacgtcc gcggccgcac tggccagagc ttcgccgagt ccggccaggc
      481 tccggcgcag tacgcccgca ccggcgagtc gcgctagaac catgaccgcc gcggtcctca
      541 ccggcccggc cgcgccgccc gcccgggaga cggcggagcc gcaggtcaac tggctgatgc
      601 tgttcctcgg cttcgggggc atggcgatcg gccagttcat ggccatcctc gacatccaga
      661 tcgtcgccgc ctccctgccg cagatccagg ccgggatcga ggccagcgcc gaccagatca
      721 gctggatcca gaccgcctac ctgatccccg aagtggtgat gatcccgctg tcggcctatc
      781 tggcccggtt gtggggcacc cagcgggtgt tcatggccag ctgcggcggt tttctgatca
      841 tgagcgtgct ggccggctgc tcgaccaaca tcgacatgat gatcgtctgc cgcgccctgc
      901 agggtttcgt cggcggagcc atggtgccga cggtgttcgc caccgccttc agcgccttcc
      961 cgccggagcg gcgggtgacc gccaacgtcg tcaccgggat gatcgtcacc ctggcgccga
     1021 ccgtcggccc gaccctgggc ggccacctga ccgactggct gaactggcgc tggctgttct
     1081 tcatcaacgt cgtgcccggc ctgctgagcc tgttcctggt cgggcgctac ggcaatttcg
     1141 acaagggcga ccggcgactg gcgcagaact tcgactggtt cggcctggcc atgatggcca
     1201 ttttcctgat gagcatgcag tacgtcgtcg aggagggcgc caaggacaac tggttccagg
     1261 ataccgccat cctctggctg acggttctgg cctcggtcac cggcgtcatc ttcgtctggc
     1321 gacagctgac ctatttccag ccgatcgtgc agctgaaggc gatcgccgac cgcaacttcg
     1381 ccctcggtct ggtgatgggc gcgacgtcgg gtctggccct ctatggcggc accttcgtgc
     1441 tgccgatcta cctgggccgg atccgcggct tttcggcgtc cgaggtcggc accaccatgc
     1501 tggtgtccgg gatttccatg ttcatcaccg gcccgctggc cggacgctgg gtgcgggcgg
     1561 tcgacccgcg cattcccatc gtcattggct acggcatggt cgcctggggc atgtggttgg
     1621 gccacgacat aaacggcgaa tggggtttcg ccgagttcgc cgccctgcag gccttccggg
     1681 gcgtgggggt gatggtggcc atgatcgcct cgacccagat gaccatgagc accctgccgc
     1741 ttcacctgat caaggacgcc tcgggcctgc tcaacctggc gcgcaacacc gccggcgcca
     1801 tcggcatggc gatcatcgcc agcaccctga cgtcccaggg cgcggtgcac atgaatgacc
     1861 tggtctcgcg catggaccgg agcagcatcg aaggccaggc gatgctggcc ggactgaccg
     1921 cccgcttcca gcagctcgga gtctccgatc cggagggcgc cgcctacaag gccatgcact
     1981 acatgatttc ccagaaggcc cagattctgg ccttcggcga cgtcttcgcc ttcatggccg
     2041 tctgcgcgat gatcgcggcc ctgctgtcca ttctggccaa gccgggaaag gtgccgcccg
     2101 gcggccgcgc gctgacgccg atcgaggatc actgacatgc cccgacttgt cggacagatc
     2161 gacgccgtca agagcgaggc cattctcgac gcggcctccg aggtcctcgg cgagcgcggc
     2221 ctgtcggcgc cgatggagga gatcgcccgc cgcgccggcg tgtccaagca gaccgtctac
     2281 aaccactatg gatgcaaggc cgacctggtc cgggcgctgg tcgagcgccg cgtcaacagc
     2341 atcaccgccc ccctgcgcga agcgggcgcc gaggaccgcc ccgaagagac cctgacggcc
     2401 tatgcccgca ccctgctgtc ggtggtcaac aaccgcagct acgccctgat gcgcgtgacc
     2461 atccaaggcg ccggcgagat gcccgagctg gcccgtgagg tgttcgaggc cggacccaag
     2521 cactcccggg cgcagctggc ccggtttctc gaggtcgaga ccctggccgg ccgtctgcgc
     2581 atcggcgatc ccatgcaggc ggcggagttc ttcggcggca tggtcctggg ccaccgccag
     2641 accaaggcgc tgctcaacat cgcgcccgac ctgaccgagc acgaggtcga ggcgatcgcc
     2701 gccgaggcgg tgcgggtctt catgcgggcc tacgcgccct gaagcgcggg gccggcggtc
     2761 agccctcggc gagatctccg tccggcagac cgatcatgtg gaagcccgca tcgacgtgaa
     2821 ccacctcacc ggtcgtcgag cggccgagat ccgacagcag ccagagcgcc gccccggcga
     2881 ccccctccat cgaggtgtcc tccttcaggg cgctgaaggc ccggccctgg ctgatcatgc
     2941 cgcggccgcc ggagatgccg gccagcgaca gggtgc
//
LOCUS       DAORNX010000369         2973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25112_length_2973_cov_7.114805, whole genome shotgun sequence.
ACCESSION   DAORNX010000369 DAORNX010000000
VERSION     DAORNX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25112_length_2973_cov_7.114805"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLO65_13790"
     CDS             complement(<1..221)
                     /locus_tag="PLO65_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29366.1"
                     /translation="MVALAVALRLTVLPGYMIATPSGGDAAVVMCTADGAVVRTVVPA
                     GTPSAPDEHRDEHPGKTSVCPFASAGVSLG"
     gene            518..>1870
                     /locus_tag="PLO65_13795"
     CDS             518..>1870
                     /locus_tag="PLO65_13795"
                     /inference="COORDINATES: protein motif:HMM:NF025184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29367.1"
                     /translation="MPKVILTEPFIRSAATLAVGCCALAAGATALPLLTTPVIGMASV
                     VGATALAKLFKDKPECEKAVTAAFKTFPKELVLSGEADEKATQEARDALKAALDRNGL
                     FTADSLEGALQDTANVVEGLTALALRSVIRADPSFAAPGFRYDLALDVLGRAFKAART
                     SCQLFREQAGLTLAEITLAEVRDGFDAAAADRLAKQEELKAYIGEVLGQQLGPESPLA
                     IIADLLAKQKLPSPKQEMEEITAASNVAPAAEAPRLAAKEAMSRNDIAGALAFMQLAE
                     ELEAREEARALADARDAQAKAETVTLSRAHTQATLASLFASQGDLKRAADLYRQAFQR
                     ASANDPESARQYARNLRIAVNKYCAQQRDFSVTRSQIDNLSTSIAPDVYSFAILIAQA
                     PDYPAARAAFDEMIAAGEKPNVVTFSTLIARAPDYPAARAVFDEMIAAGEKPNVVTFS
                     T"
     assembly_gap    1871..1970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1971..2551
                     /locus_tag="PLO65_13800"
     CDS             <1971..2551
                     /locus_tag="PLO65_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29368.1"
                     /translation="AARAVFDEMIAAGEKPNVFVASSIASRLETIEQATELRDVLRAE
                     SRAGTTFYQAAFARLCRQYAARPILNWALGDGKGLPFQAFDAAISTYRRRGDLDSAFL
                     IAAGFPYLEASRTVFRAFGEQARAYLEARFEGGVEPHIAARALGVVFAYAKDTDESLR
                     WFDIALRHEMTPPARVAVIQAEIDRVRLSEFA"
     gene            2576..>2973
                     /locus_tag="PLO65_13805"
     CDS             2576..>2973
                     /locus_tag="PLO65_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD29369.1"
                     /translation="MRRLAVPALTAAFATSALAMPPPLPDVANASMAELQVRVEAGEA
                     SYESLASAYIVRSRELDPKLRAIIAVNPNALTDARAADLGFRADAAAADGRRGTPLFG
                     MPILIKDNIETLDPMATTAGSLALAGNVTGR"
BASE COUNT          524 a          968 c          911 g          470 t
ORIGIN      
        1 cccagggaga cgccggccga ggcgaagggg cagaccgagg tcttgcccgg atgctcgtcg
       61 cgatgctcgt cgggcgccga cggggttccg gccggaacga ccgtccggac gaccgcgccg
      121 tcggccgtgc acatcaccac cgccgcgtcg ccgccggagg gcgtcgcgat catgtagccg
      181 ggcaggacgg tcaggcgcag ggcgaccgcc agggcgacca tcgcccatgc gctccgcccc
      241 cagcggactc tgtccttcgg ccaccccgtc atcgcgcccg tcggttatag cgggactcgc
      301 cgggacacaa tgatcaggat caggtcgcga cgcgggactt gcccccgtcg ccatccgccg
      361 ccttgatgga gatcccacac agtccgcatg gcaggcccag gtggccgcct gcaaggccgg
      421 cgacaggagc gcctgcgcgc cgaagtaagg acactggcgc gaacggtttg cgtcaaccat
      481 atttctcccg tcggttgcgg gagcatcagt tccagtcatg ccaaaggtca tcctgaccga
      541 accgttcatc aggtcggccg ccacgctggc ggtcggctgc tgcgcgttgg cggcgggggc
      601 tacggccttg ccgctgctga ccacgccggt gatcggcatg gcatcggtgg ttggcgcgac
      661 cgctctcgcc aagctcttca aggacaagcc cgagtgcgag aaggcggtga cggccgcctt
      721 caagactttc ccaaaggagt tggtactcag cggtgaagcc gacgaaaagg ccacgcagga
      781 ggcgcgcgac gccttgaagg cagcactcga ccggaacggt ctcttcacgg cggacagcct
      841 tgaaggcgcg ctccaggaca cggccaacgt cgtcgaaggt ctgacggctc tggcgctgcg
      901 gagcgtgatc cgcgccgacc cgagcttcgc cgcccccggc ttccgttacg atttggcctt
      961 ggacgtcctt ggccgcgcct tcaaggcggc ccggacgagc tgccaacttt tcagggaaca
     1021 ggctggcctg acgctggcgg agatcacgct ggcggaggtt cgggatggct tcgacgccgc
     1081 ggcggcggat cgcttggcga agcaggaaga actcaaggcg tacatcggcg aggtcctcgg
     1141 ccaacagctg ggccccgaga gcccgctcgc gatcattgct gacctgcttg ccaagcagaa
     1201 actaccgtcg ccgaaacagg agatggagga gattactgcg gcaagcaacg tcgcgcccgc
     1261 cgcagaagcg ccgcgactcg ccgcgaaaga ggccatgtca cgcaatgata tcgcgggggc
     1321 tctcgccttc atgcagcttg ccgaggaact cgaggcacgt gaggaagcac gagcgctggc
     1381 cgatgctcgg gatgcccaag cgaaggccga gactgtcaca ctttcccgtg cccatactca
     1441 agcgacccta gcgtcgcttt tcgctagcca aggcgacctc aaaagagccg ccgacctgta
     1501 ccggcaagcc ttccaaaggg cctcagccaa cgatccggaa tcggctcgcc agtatgcgag
     1561 gaaccttcga attgccgtca acaagtactg tgctcaacaa cgagacttca gcgtaacacg
     1621 gtcccagatt gacaacctgt cgacgagcat cgccccggac gtatattcct ttgcgattct
     1681 aatcgcccag gcccccgact accccgcagc tcgggccgcc ttcgacgaga tgatagcggc
     1741 tggcgaaaag cccaatgtcg taaccttcag caccctcatc gcccgggccc ccgactaccc
     1801 cgccgctcgg gccgtcttcg acgagatgat agcggctggc gaaaagccca atgtcgtaac
     1861 cttcagcacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgccgctcg
     1981 ggccgtcttc gacgagatga tagcggctgg cgaaaagccc aatgtgttcg tggcctcgtc
     2041 gatcgcaagt cgcctcgaga caatcgagca ggccaccgaa ttgagagatg ttctgcgggc
     2101 tgaaagtcgc gccggcacaa cgttctacca ggcagcattc gctcgactgt gccgccaata
     2161 tgccgcgcgg ccaatcctga attgggcgct cggggacggg aagggccttc cgttccaagc
     2221 ctttgatgcc gccatctcga cctatcggag acgtggcgat ttggattccg ccttcctaat
     2281 cgctgcaggc tttccctacc ttgaggcctc aaggacagtg ttccgcgcct tcggcgaaca
     2341 agcacgcgcc taccttgagg cgcgctttga agggggcgtt gagccgcaca tagccgctcg
     2401 cgcactcgga gttgtctttg cctatgcaaa ggataccgat gaatctctgc ggtggtttga
     2461 catcgcgctg agacacgaaa tgacgccacc tgctcgagtg gcggtgattc aggctgagat
     2521 tgatcgtgtt cgcctcagtg agttcgcgta gcgtcacgtc ttgaggagcc gtcccatgcg
     2581 ccgtctcgcc gtccctgccc tgaccgccgc gttcgccacg tcggccctgg ccatgccgcc
     2641 gcccctgccg gatgtggcca acgcctcgat ggccgagctg caggtgcggg tcgaagcggg
     2701 cgaggcaagc tacgagagcc tggccagcgc ctacatcgtg cggagccggg agctcgatcc
     2761 gaagcttcgc gcgatcatcg cggtcaaccc gaacgccctg actgacgccc gcgccgccga
     2821 cctggggttc cgggccgacg cggcggcggc ggacgggcgg cgcgggaccc ccctcttcgg
     2881 catgccgatc ctgatcaagg acaacatcga gaccctcgac ccgatggcga ccaccgcagg
     2941 gtcgctggcc ctggccggca atgtcaccgg ccg
//
LOCUS       DAORNX010000370         2973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25113_length_2973_cov_6.754626, whole genome shotgun sequence.
ACCESSION   DAORNX010000370 DAORNX010000000
VERSION     DAORNX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25113_length_2973_cov_6.754626"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..380
                     /locus_tag="PLO65_13810"
     CDS             132..380
                     /locus_tag="PLO65_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29370.1"
                     /translation="MAVQTVHLVQAFIAGRGKALKSEQAVVCKTAEEAMRRAERLAPL
                     RLGVIAFSATGDMEMGDFDETPTILFRSGRLPPPFDEG"
     gene            complement(410..760)
                     /locus_tag="PLO65_13815"
     CDS             complement(410..760)
                     /locus_tag="PLO65_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRD29371.1"
                     /translation="MRRAPTLNERALWKLLRNRRLDGLKFRRQVPIGRYIVDFLCLRH
                     RLIVEADGPTHDDSLRDQERDAWLAGQGFHVMRFANAEIERWPSRVLDAICAAVAAPP
                     ADDRGGPPHPSGFA"
     gene            complement(929..>2973)
                     /locus_tag="PLO65_13820"
     CDS             complement(929..>2973)
                     /locus_tag="PLO65_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD29372.1"
                     /translation="IGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRIV
                     NGDVPESLKDKKLLSLDMGSLIAGAKYRGEFEERLKAVLGEVTAAEGSIILFIDEMHT
                     LVGAGKSDGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVFVGE
                     PTVEDTVSILRGLKEKYELHHGVRISDSAIVAAATLSNRYIADRFLPDKAIDLMDEAA
                     SRVRMAVDSKPEELDEIDRRLVMLKIEREALKKETDAASRQRLENLETEIDDLQFQSD
                     EMTARWKSEKEKVGSAAQVREALERLRAELAAAQRRGDLQRASEIAYGEIPQIEKRLA
                     EEDGKAAENPLSPEVVDAEQIAAVVSRWTGVPVDKMLESEREKLLKMEDALRGRVVGQ
                     EEALVAVSDAVRRARAGLQDPGRPIGSFLFLGPTGVGKTELTKALAEFMFDDEAAITR
                     LDMSEYMEKHSVSRMIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEVEKAHPDVFNI
                     LLQVLDDGRLTDGQGRTVDFRNTVIILTSNMGSEFLAGLDEGQDVEVVRPQVMEVVRR
                     SFRPEFLNRLDEIILFKRLGRGEMDNIVRIQLQRVEKLLADRRMSIALDSAATHWLAE
                     RGYDPVYGARPLKRVIQKELMDPIARKLLAGDMADGSVIEVSADADGLVIGKARVH"
BASE COUNT          427 a         1044 c          988 g          514 t
ORIGIN      
        1 ggcgagccgg tgccgctgct gcggatgggg aaggggctct gaggcggccg aaagggcgcc
       61 cgccgactcg gccgcgggtg ccacccaggc cgtcgcgacc gcgccgggcg tccgcgtacc
      121 cggagctccc catggccgtc cagaccgtcc acctcgtcca ggccttcatc gccggccgcg
      181 gcaaggccct gaagtcggag caggcggtag tctgcaagac ggccgaggag gcgatgcgcc
      241 gcgccgaacg cctggccccc ctgcggctgg gcgtgatcgc cttcagcgcc accggcgaca
      301 tggaaatggg cgacttcgac gagaccccga cgatcctgtt caggagcgga aggctgcccc
      361 cgccgtttga cgaggggtga ttccccttct ccccttgcgg gagaaggtgt caggcgaagc
      421 ctgacggatg agggggccca ccgcgatcat cggccggcgg cgctgcgacc gccgcgcaaa
      481 tggcgtcaag cacccggctc ggccaccttt caatctcggc attggcaaac cgcatgacgt
      541 gaaagccctg acccgcgagc caggcatcac gttcctgatc acgcaggctg tcatcgtgag
      601 tgggaccatc ggcctcgacg atcagccgat gccgcaggca aagaaaatcg acgatgtatc
      661 gcccgatcgg gacctgtcgt cgaaacttga ggccatcgag gcgccggttg cgcaacagtt
      721 tccacaacgc gcgctcgttt agcgtcggcg cacggcgcat gcgtttcgcg aaggcgagtc
      781 tagcggaatc gcacggcatg cgtgcaacct agcgcgattg cccttctggg tgaagagggc
      841 ggggaattgg gtcgtttgcg ggtccgttgg acccctcatc cgacccgctt cgcgggccac
      901 cttctcccgc aaggggagaa ggagggcctt aatgcaccct tgccttcccg atcaccaggc
      961 cgtccgcgtc cgctgacacc tcgatcacgc tgccgtccgc catatccccg gccagcagct
     1021 tgcgcgcgat aggatccatc agctccttct ggatcacccg cttcagcggc cgggccccgt
     1081 agaccgggtc gtagccgcgt tcggccagcc agtgggtggc ggcgctgtcg agggcgatgc
     1141 tcatgcggcg gtcggccagc agcttctcga cccgctgcag ctggatcctg acgatgttgt
     1201 ccatctcccc gcggcccagc cgcttgaaca ggatgatctc gtccaggcgg ttgaggaact
     1261 cgggacggaa gctccgccgg accacctcca tcacctgcgg ccgcacgacc tccacgtcct
     1321 ggccctcgtc gagcccggcg aggaattcgc tgcccatgtt gctggtcagg atgatcacgg
     1381 tgttgcggaa gtcgaccgtg cggccctggc cgtcggtcag ccgcccgtcg tcgagcacct
     1441 gcagcaggat gttgaacacg tccggatggg ccttctcgac ctcgtcgaac agcaccacct
     1501 ggtagggccg gcggcgcacg gcctcggtca gggcgccgcc ctcgtcatag ccgacatagc
     1561 cgggaggggc cccgatcatg cggctgaccg agtgcttctc catgtactcg ctcatgtcga
     1621 gccgggtgat cgccgcctcg tcgtcgaaca tgaattcggc cagcgccttg gtcagctcgg
     1681 tcttgcccac gcccgtcggg ccgaggaaca ggaagctgcc gatcggccgg ccgggatcct
     1741 gcagcccggc gcgggcgcgg cgcacggcgt cggacacggc gaccagcgcc tcttcctgcc
     1801 caaccacgcg gccacgcagg gcgtcctcca tcttgagcag cttctcgcgc tcgctctcga
     1861 gcatcttgtc gacgggcacg ccggtccagc ggctgaccac ggcggcgatc tgctcggcgt
     1921 cgaccacctc ggggctcagc gggttttcgg cggccttgcc gtcttcctcg gccaggcgct
     1981 tctcgatctg cgggatctcg ccataggcga tctccgacgc ccgctgcagg tcgccgcgac
     2041 gctgggcggc ggccagttcc gcgcgcagcc gctcgagcgc ctcgcgcacc tgggcggcgc
     2101 tgccgacctt ttccttctcc gacttccagc gggcggtcat ctcgtcggac tggaactgca
     2161 ggtcgtcgat ctcggtctcg aggttctcca gccgctgccg cgaggccgcg tcggtctcct
     2221 tcttcagggc ctcgcgctcg atcttcagca tgaccagccg acggtcgatc tcgtccagct
     2281 cctcgggctt ggagtcgacg gccatgcgca cgcggctggc cgcctcgtcc atcaggtcga
     2341 tggccttgtc cggcaggaag cggtcggcga tgtagcggtt ggacagggtg gcggccgcga
     2401 cgatggcgct gtcgctgatc cgcaccccgt ggtgcagctc gtacttctcc ttcaagccgc
     2461 gcaggattga gacggtgtcc tcgaccgtcg gctcgccgac gaacacgggc tggaagcgac
     2521 gggcgagcgc cgcgtccttc tcgacgtgct tgcggtactc gtcgagcgtg gtggcgccga
     2581 cgcagtgcag ctcgccgcgg gccagggccg gcttgagcag gttggaggcg tccatcgccc
     2641 cgtcgctctt cccggcgccg accagggtgt gcatctcgtc gatgaacagg atgatgctgc
     2701 cctcggcggc cgtgacctcg ccgagcacgg ccttcagccg ctcctcgaac tcgccgcgat
     2761 acttcgcacc ggcgatcagg ctgcccatgt cgagtgacag cagcttcttg tccttcaggc
     2821 tctcgggcac gtcgccgttg acgatgcgca gggccaggcc ctcgacgatg gcggtcttgc
     2881 caacgcccgg ctcgccgatc agcacggggt tgttcttggt ccggcggctc agtacctgga
     2941 tggtgcggcg gatttcctcg tcgcggccga tca
//
LOCUS       DAORNX010000371         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25128_length_2972_cov_5.141241, whole genome shotgun sequence.
ACCESSION   DAORNX010000371 DAORNX010000000
VERSION     DAORNX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25128_length_2972_cov_5.141241"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLO65_13825"
     CDS             complement(<1..309)
                     /locus_tag="PLO65_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRD29373.1"
                     /translation="MSDAFKPLLSRLADGATLSEADAESFFSACLRGEPTPAQVAAAV
                     TAIRLRGETVGEIVACARAMRRAAVNLDLPYETVDVCGTGGDGLHTLNISTATAFVAA
                     G"
     gene            complement(315..905)
                     /locus_tag="PLO65_13830"
     CDS             complement(315..905)
                     /locus_tag="PLO65_13830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRD29374.1"
                     /translation="MILVIDNYDSFTYNLVHYLNELGAETVVYRNDALTVQEALGLRP
                     EAVLLSPGPCAPDQAGICLPLLRGAPEDLPILGVCLGHQAIGQAYGGEVVRAKAIMHG
                     KTSPIHHTDKGLFKGMPNPFTAARYHSLSVPRETLPEVLEVTAWTDDGEIMGFQHRTR
                     PVFGVQFHPESIATEGGHHLLANFLDLAGVKRVETA"
     assembly_gap    1142..1168
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1181..1384
                     /locus_tag="PLO65_13835"
     CDS             1181..1384
                     /locus_tag="PLO65_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29375.1"
                     /translation="MAYARGLLKPPLEPDGLMGALGAALFAAIAALGFAVAMVMAPPV
                     TTSKLALEPAPASAGVVSANENN"
     gene            complement(1514..1912)
                     /locus_tag="PLO65_13840"
     CDS             complement(1514..1912)
                     /locus_tag="PLO65_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29376.1"
                     /translation="MIPARLLAVCVSALIAGSAVAQTAPEPAPPPGARFIAKPKWKRL
                     PSGGDIAGQVSRPTAGAKIGGAVVIECIVGAGGRLVDCKVLAERPEGQGFGEAGLRLT
                     SKFVMAPRDKDGLPTEGGFVRIPLSFFPTH"
     gene            complement(1924..>2972)
                     /locus_tag="PLO65_13845"
     CDS             complement(1924..>2972)
                     /locus_tag="PLO65_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRD29377.1"
                     /translation="KGVSAEAAWAEANARLDAVMADLRLPLPAPAPEGAPGPDVFETR
                     VSRAAYADIVGKAKAYIRAGDIFQVVPSHRFRAPFKAGDAFQIVPSQRFSMPFKLPPL
                     ALYRALRRINPSPFLIYFDFGGFSIVGSSPEILVRLRDGKITIRPIAGTRPRGATPEQ
                     DAALEAELLADPKERAEHLMLLDLGRNDVGRVARLREPGRNEPAKASRGPAVRVTESF
                     VIERYSHVMHIVSNVEGDAPEGLDPVDVLMAALPAGTLSGAPKVRAMEIIDELEIEKR
                     GLGYAGAVGYFGADGSVDTCIVLRTALVKDGTMYVQAGGGVVADSDPDAEYDETLHKS
                     NALRRAAAEAWRFG"
BASE COUNT          426 a         1033 c         1055 g          431 t
ORIGIN      
        1 gccggccgcc acgaaggcgg tggcggtgga gatgttcagg gtgtgcaggc cgtcgccgcc
       61 ggtgccgcag acgtcgacgg tctcgtaggg caggtccagg ttgaccgccg cccggcgcat
      121 ggcccgggcg caggcgacga tctcgccgac ggtttcgccg cgcaggcgga tggccgtgac
      181 cgccgcggcc acctgggccg gggtcggctc gccgcgcagg caggcgctga agaaggactc
      241 ggcgtcggct tccgacaggg tcgcgccgtc cgccagacgg ctcagcaggg gcttgaaggc
      301 gtcggacacg tcgctcaggc cgtctcgacg cgcttcacgc cggccagatc gaggaaattg
      361 gccagcaggt ggtggccgcc ctcggtggcg atggactcgg ggtggaactg cacgccgaac
      421 accggccgcg tccgatgctg gaagcccatg atctcgccgt cgtcggtcca ggcggtcacc
      481 tccagcacct cgggcagggt ctccctcggc accgacaggc tgtgatagcg cgcggccgtg
      541 aacgggttgg gcatgccctt gaacagcccc ttgtcggtgt gatggatcgg gctggtcttg
      601 ccgtgcatga tcgccttggc ccgcacgacc tcgccgccgt aggcctggcc gatggcctgg
      661 tggcccaggc agacgccgag gatcggcagg tcctccggcg cgccgcgcag caacggcagg
      721 cagatccccg cctggtccgg cgcgcacggc cccggcgaca gcagcacggc ttccggcctg
      781 aggccgaggg cttcctgaac cgtcagcgcg tcgttgcgat agacgaccgt ctccgccccc
      841 agctcgttca ggtagtgaac gagattgtag gtaaagctgt cgtagttatc gatgacgagg
      901 atcatggcgc tctgcttcta atgcctttcc ccgcaaccgt catcgcccca atgataaacc
      961 gccaccccaa acctccgtca tcgtcggacc tgttccgacg acccatgcgc tcagtgcgga
     1021 ccgtccgtgg cgcggcgccc accgcaaatc ccatcaatca actaggcctt gggtcgtcgg
     1081 aacaagtccg acgatgacgg gggaggggaa acgcggtttg cgccagaggc cgccgcgctt
     1141 cnnnnnnnnn nnnnnnnnnn nnnnnnnnga ccgcgaggcc ctggcctacg ccagggggct
     1201 gctcaagccg ccgctggagc cggacggcct gatgggcgcc ctgggcgcgg ccctgttcgc
     1261 ggcgatcgcg gcgctcggct tcgccgttgc catggtcatg gccccgccgg tgaccacgag
     1321 caaactggcg ctggagcccg cgcccgcgag cgccggggtc gtgtcggcca acgagaacaa
     1381 ctagcgccgg gcggtggcct tcagcccgcg atcggggccg ccagcgcggc gaccggcggt
     1441 ctcggttccc cgggtccgtc ccttccggca ggcctgtccc tggacaggcg ggccgcgggg
     1501 ggaggtcgag cgcctaatgc gtcggaaaga aactcagcgg gatccggaca aagccaccct
     1561 cggtcgggag cccgtccttg tctcgaggcg ccataacgaa cttgctcgtc agcctcaggc
     1621 cggcctcgcc gaagccctgc ccttcagggc gctcggccag caccttgcag tccacgaggc
     1681 gccccccggc gcccacgatg cattcgatca cgacagcccc gccgatcttc gcccctgcgg
     1741 tcgggcgtga gacttgtcca gcgatgtcgc cgcctgaggg caggcgcttc catttcggct
     1801 tggcgatgaa gcgcgcgccc ggtggaggcg cgggctcggg ggcggtctga gcaaccgcag
     1861 agccggcgat cagggcgctg acgcatacag cgaggaggcg ggccgggatc atgaaaggac
     1921 ctcctacccg aaccgccagg cctccgccgc cgcgcggcgc aacgcgttcg acttgtgcag
     1981 ggtctcgtcg tactcggcgt ccgggtcgct gtcggcgaca accccgccgc cggcctggac
     2041 gtacatcgtc ccgtccttga ccagggccgt gcgcagcacg atgcaggtgt cgaccgagcc
     2101 gtcggcgccg aagtagccga ccgccccggc gtagccgagg ccgcgcttct cgatctccag
     2161 ttcgtcgatg atctccatcg cccgcacctt gggcgcgccg gacagggtgc cggcgggcag
     2221 ggcggccatc agcacgtcga ccgggtccag cccctcgggc gcgtcgccct cgacgttgga
     2281 gacgatgtgc atcacgtggc tgtagcgctc gatgacgaag ctctcggtca cccgcaccgc
     2341 cgggccgcgc gaggccttcg ccggttcgtt gcggcccggc tcgcgcaggc gggccacccg
     2401 gcccacatcg ttgcggccca ggtccagcag catcaggtgc tcggcccgct ccttgggatc
     2461 ggccagcagc tcggcctcga gcgccgcgtc ctgctcgggg gtggccccgc gcggccgggt
     2521 gccggcgatc ggccggatgg tgatcttgcc gtcgcgcagc cgcacgagga tctccgggct
     2581 ggagcccacg atggagaagc cgccgaagtc gaagtagatc aggaacggcg acgggttgat
     2641 gcgccggagc gcgcgataga gcgcgagcgg cggcagcttg aagggcatcg agaatcgctg
     2701 cgagggcacg atctggaacg cgtcgccggc cttgaacggc gcgcgaaagc ggtggctcgg
     2761 cacgacctgg aagatgtcgc cggcgcggat gtaggccttg gccttgccga cgatatcggc
     2821 gtaggccgcg cgactgaccc tggtctcgaa aacgtccggg ccgggcgcgc cttccggcgc
     2881 cggcgcgggc agggggaggc gcaggtcggc catcaccgcg tccagccggg cgttggcttc
     2941 ggcccaggcc gcttcggcgc tgacgccctt ca
//
LOCUS       DAORNX010000372         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25549_length_2933_cov_6.384990, whole genome shotgun sequence.
ACCESSION   DAORNX010000372 DAORNX010000000
VERSION     DAORNX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25549_length_2933_cov_6.384990"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLO65_13850"
     CDS             complement(<1..150)
                     /locus_tag="PLO65_13850"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRD29378.1"
                     /translation="MTDTIFAPATAPGRAALAVVRISGPETGDLLEKLAGGRPAPRRA
                     SLRRLT"
     gene            complement(147..401)
                     /locus_tag="PLO65_13855"
     CDS             complement(147..401)
                     /locus_tag="PLO65_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HRD29379.1"
                     /translation="MKVTINIECDPVEARTFLGLPDVSGLNDHLVEEMKKRMEANMAA
                     LAPDELMKSWMSFGTGATEQFRKLMTAATAGAGFGTKPPE"
     gene            497..1195
                     /locus_tag="PLO65_13860"
     CDS             497..1195
                     /locus_tag="PLO65_13860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD29380.1"
                     /translation="MSGERLTIKTPDGEFSAYVARPAAATAPAVVVIQEIFGVNAVMR
                     ETTDWLAAQGYLAICPDLFWRIEPGIDITDRTKAEWDRAFELFGLFDAEKGVADIAAT
                     IAAVRADRGCNGKVGAVGYCLGGKMAYLTAARTDVDASVSYYGVGLDALTAEAATIKK
                     PLLMHIAGEDGFVPKEAQAAIHAALDGNPLITIHDYPGRDHAFARHGGEHYDAADAGK
                     ANDRTLAFFKANLG"
     gene            1195..2166
                     /locus_tag="PLO65_13865"
     CDS             1195..2166
                     /locus_tag="PLO65_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRD29381.1"
                     /translation="MLAIQATRAGGPEVLEAVDLPTPTPGPGEILIRQEAVGLNYIDT
                     YQRTGLYPVPFPTVLGLEGAGTVEAVGEGVTRFAVGDRAAYGNGPLGAYAEYHVVPAG
                     RAVKAPSAIDSRTAAAAMLKGMTAEFLLRRCYPVRKGDAVLIHAAAGGVGSILTQWAR
                     ALGATVIGTAGSPEKAALARNFGADETILYREEDVAARVRAITGGAGVRVVYDGVGAA
                     TFEGSLASLGRRGMLVSYGNASGPAPAVEPLRLSRGGSLFLTRPTLFDYVVTTEELDE
                     SAGALFEVINSGAVKIDIGQTFPLSQTRQAHEALEGRETTGSTLLIP"
     gene            complement(2320..>2933)
                     /locus_tag="PLO65_13870"
     CDS             complement(2320..>2933)
                     /locus_tag="PLO65_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD29382.1"
                     /translation="GPAGGVLGIDGDAVKIAAAREAGAGLANLAFEVGDLTDPDGGAA
                     FDVVYARFLLSHLTDPDRVVASLGGRIRSGDALIVEDVDFDGHFVDPPQPAFDRYVRW
                     YQEAAGRRGANPRLGRELPRRIVAAGFDILHAGAVSPAALDGPVKQIAPLTLAAIAEP
                     VVAAGLASTGEVERTLAELQAAADDPGVFMSLPRVVQVIARRI"
BASE COUNT          443 a         1053 c         1018 g          419 t
ORIGIN      
        1 ggtcagccgc cgcagcgagg cccggcgcgg cgccggccgg ccgccggcga gtttctccag
       61 caggtcgccg gtctccggtc ccgagatccg caccacggcg agagccgcgc gcccgggcgc
      121 cgtcgccggg gcgaagatgg tgtccgtcat tccggcggct tggtcccgaa gccggccccg
      181 gcggtggcgg cggtcatcag cttgcggaac tgttcggtcg cgccggtgcc gaaactcatc
      241 cagctcttca tcagctcatc cggcgccagc gcggccatgt tggcctccat gcgcttcttc
      301 atctcctcga ccagatggtc gttgagcccg ctgacgtcgg gcaggccgag gaaggtgcgg
      361 gcctccacgg gatcgcattc gatgttgatc gtgaccttca tgggtcttcc ttgatggcgc
      421 gcgacccaac tttattgtgg gcgagcgggg ccctgccgcg ttaacacgcc gcggacagcg
      481 acgggagacc aggacgatga gcggagaacg cctgaccatc aagacgccgg atggcgagtt
      541 ttcggcctat gtggcccggc cggccgcggc cacggcgccg gcggtggtcg ttatccagga
      601 gatcttcggg gtcaacgcgg tgatgcgcga gaccaccgac tggctggcgg cccagggcta
      661 cctggccatc tgccccgacc tgttctggcg catcgagccg ggcatcgaca tcaccgaccg
      721 gaccaaggcc gaatgggatc gtgccttcga actgttcggc ctgttcgacg ccgagaaggg
      781 cgtcgccgat atcgccgcga cgatcgccgc cgtccgcgcc gaccggggct gcaacggcaa
      841 ggtcggggcc gtcggctact gcctgggcgg caagatggcc tatctgaccg ccgcccgcac
      901 cgatgtcgac gcctcggtct cctactacgg cgtcggcctc gacgccctga ccgccgaggc
      961 ggcgacgatc aaaaagcccc tgctcatgca catcgccggc gaggacggct ttgtgccgaa
     1021 ggaggcccag gcggcgatcc acgcggcgct cgacggcaac ccgttgatca cgatccacga
     1081 ctaccccggg cgtgaccacg ccttcgcccg tcacggcggc gagcattacg acgcggcgga
     1141 cgccggcaag gccaacgacc gcaccctggc cttcttcaaa gcgaacctcg gctgatgctg
     1201 gcgatccagg cgacccgcgc cggcggtccc gaggtgctcg aggccgtcga cctgccgacc
     1261 cccacgccgg ggccggggga aattctcatc cgccaggagg ccgtgggcct caactatatc
     1321 gacacctacc agcgcacggg cctctatccg gtcccgttcc cgacggtgct cggcctcgag
     1381 ggcgcgggaa cggtggaggc ggtcggcgag ggcgttaccc gtttcgcggt cggcgatcgc
     1441 gcggcctacg gcaacggccc cctcggcgcc tacgccgagt atcatgtcgt accggctggc
     1501 cgcgccgtga aggcgccgtc cgccatcgac agccgcaccg ccgccgcggc catgctcaag
     1561 ggcatgacgg ccgagttcct gctgcggcgc tgctacccgg tgagaaaggg cgacgcggtg
     1621 ctgatccacg cggccgccgg cggggtcgga tccatcctca cccagtgggc cagggcgctt
     1681 ggcgcgacgg tcatcggcac ggccggatcg cctgaaaagg ccgccctggc caggaatttc
     1741 ggggccgacg aaaccatcct ctaccgcgag gaggacgtcg ccgcccgggt gcgggcgatc
     1801 accggcggcg ccggcgtccg cgtcgtctac gacggcgtcg gcgcggcgac cttcgagggc
     1861 agcctcgcca gtctcggccg gcggggcatg ctggtcagct acggcaacgc gtccgggccg
     1921 gcgcccgccg tcgagccgct gcgcctgtcg cgcggcgggt cgctgttcct gacccggccg
     1981 acgctgttcg actatgtcgt cacaaccgag gagctggacg agagcgccgg cgccctgttc
     2041 gaggtgatca actcgggcgc ggtgaagatc gacatcggcc agacctttcc gctcagccag
     2101 acccgccagg cgcacgaggc cctcgagggc cgcgagacga ccggatcgac gctgctgatc
     2161 ccctagagcg cgttccgatt agtgggaacc ggcaatcggg taaaacgcgc gaccacgaaa
     2221 ggcctggagc agggcgattt gattccatca aatcgcgtcc tgctctagat ccttgcggtc
     2281 tgccgaggcg gccggacgtc gcatcggccg cggcgcgcgt cagatccgtc tcgcgatgac
     2341 ctggaccacc ctgggcaggc tcatgaacac gccgggatcg tcggcggccg cctgcagctc
     2401 tgcgagcgtc ctctcgacct ccccggtcga cgccagcccc gccgccacga ccggctcggc
     2461 gatcgccgcc agggtcagtg gcgcgatctg cttgaccggc ccgtccagcg cggccggact
     2521 gacggcgccg gcgtggagga tatcgaaccc cgcggcgacg atcctgcgag gcagttcccg
     2581 gccgaggcgc gggttcgcgc cacgccgtcc ggcggcttcc tggtaccagc ggacatagcg
     2641 gtcgaacgcc ggctgtggcg ggtcgacgaa atgcccatcg aagtcgacat cctcgacaat
     2701 cagggcgtcg ccggaacgga tccggccgcc gagcgaagcc accacccggt cgggatccgt
     2761 cagatgggac aacaggaagc gggcgtagac cacgtcgaag gccgccccgc cgtccgggtc
     2821 ggtcaggtcg ccgacctcga aggccaggtt ggccagaccg gcgccggcct cgcgcgcggc
     2881 ggcgatcttc acggcgtcgc catcgatccc cagcaccccg ccggcggggc cga
//
LOCUS       DAORNX010000373         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25601_length_2929_cov_8.462074, whole genome shotgun sequence.
ACCESSION   DAORNX010000373 DAORNX010000000
VERSION     DAORNX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25601_length_2929_cov_8.462074"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLO65_13875"
     CDS             <1..308
                     /locus_tag="PLO65_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29383.1"
                     /translation="SLWRPMGDEHGMIIHPSGLPALKGGDFVRAVDALDADPAAPWPE
                     DAPKPGGLVWLQPGHCDPTINLYDALHVVSDDGAIERWAIDARRSSRAGVRSRAGEE"
     gene            373..1104
                     /locus_tag="PLO65_13880"
     CDS             373..1104
                     /locus_tag="PLO65_13880"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29384.1"
                     /translation="MNDLDFTGRRVLVVGGTSGIGNGVAQAFRERGAKVAVWGTREAA
                     DYRAEDGSDLAGLAFARVDVSDSAAVTAAPSPFDGLDVLVLAQGTVLYRKGEFEMDGF
                     RKVVGVNLDSMMACSMRFLADLKASGGNIVTISSTSAYHATRGNPAYNASKAGVVALT
                     RTLGQAWATDGVRVNGVAPGLVDTKLTKVTTEHPDRLAGALRGIPMHRLGTPQDMAGA
                     VMFLASPLAAYITGQTIIVDGGLTL"
     gene            1180..2829
                     /locus_tag="PLO65_13885"
     CDS             1180..2829
                     /locus_tag="PLO65_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HRD29385.1"
                     /translation="MKKLLLAALVAAIPLAAHAADPVVNPGHLATDIKVLSSDAFEGR
                     GPATPGGDKAVAWIIGQMIAAGLEPGGDLKDGKRAWTQDVPLGRFEMKGPVTSSFSVG
                     GQSIALNQGEQIAIRAAQTNVDSVSLAGLPVVFAGYGVRAPERNWDDFAGMDVKGKVV
                     IVLINDPDFESGKGDFGGKAMTYYGRWTYKYEEAARLGAAALIIVHETDPASYGWATV
                     KNSNTNTMFDIVRDKPAEVHPPVEAWIQRDVAVDLFRRAGLDFDALKKKAAKRGFKAV
                     ALPGVTFSTDFKVDHQVIVSQNVAGVLPGATRPDERVFYTAHWDHLGVGLPDASGDTI
                     YNGAVDNATGVAALLELGRMYAAAPRPDRSVVFLAVTAEEKGLLGSEYYAANPLYPLA
                     TTVADLNMDGFSPTGPSRDYTTSGDGKVSLQDDLVAALKARGRVFTPDPVPEAGHFFR
                     SDHFPFAKRGVPAISVGPGEDLVVGGVAAGKAYNDMYIKDRYHQPADEWSDDWDLSGM
                     ATDLTLLYDLGRDLATSTRWPEWQAGSEFKAVRDATAAERQ"
BASE COUNT          456 a         1061 c          983 g          429 t
ORIGIN      
        1 gttcgctgtg gcggccgatg ggcgacgagc acggcatgat catccacccg tccggtctgc
       61 ccgcgctgaa gggtggcgac ttcgtcaggg ccgtcgacgc gctcgacgcc gacccggccg
      121 ccccctggcc cgaggacgcg ccgaaacccg gcggcctggt ctggctgcag cccggccact
      181 gcgatccgac gatcaacctg tacgacgccc tgcacgtggt ctccgatgac ggcgcgatcg
      241 agcgctgggc gatcgacgcc cggcggtcga gccgggccgg cgtccggtcc cgcgccggag
      301 aggaatgatc agggtcttgc gctctccccg cgtcagcgcg gcacactcac gagatcaacg
      361 ggagcaagcg caatgaacga cctcgatttc accggcaggc gcgtgctggt cgtcggcggc
      421 accagcggca tcggcaacgg cgtcgcccag gcgttccgcg aacgcggcgc gaaggtcgcc
      481 gtctggggca cccgcgaggc cgccgactac agggccgagg acggttcgga cctggccggc
      541 ctggccttcg cccgggtcga cgtctccgac agcgccgccg tgaccgccgc gccctcgccg
      601 ttcgacggcc tcgacgtgct ggtcctggcc cagggcacgg tgctttaccg caagggcgag
      661 ttcgagatgg acggcttccg caaggtggtc ggagtcaacc tcgacagcat gatggcctgc
      721 tcaatgcgct tcctcgccga tctcaaggcc tcgggcggca acatcgtcac catcagctcg
      781 acctcggcct accacgccac ccgcggcaat ccggcctaca acgcctccaa ggccggtgtc
      841 gtggccctga cgcgtacgct gggccaggcc tgggcgacgg acggcgtgcg ggtcaacggc
      901 gtggcgccgg gcctggtcga caccaagttg accaaggtga ccaccgagca tcccgaccgt
      961 ctggccggcg ccctgcgcgg cattcccatg caccggctgg gcacgccgca ggacatggcc
     1021 ggggcggtga tgttcctcgc ctcgccgctg gcggcctaca tcaccgggca gacgataatc
     1081 gtcgacggcg gattgaccct ctgaactagg gctttgcggt gagcgatttc gggcgatagg
     1141 gtccggcccg caattcctcg ccatcaagag cgctgtttca tgaaaaagct tctgcttgcc
     1201 gccctggtcg ccgcgatccc gctggcggcc catgccgccg accccgtggt caatccgggc
     1261 cacctggcca ccgacatcaa ggtgctgtca tcggacgcct tcgaggggcg cggcccggcc
     1321 acgccgggcg gcgacaaggc cgtcgcctgg atcatcggcc agatgatcgc cgcgggcctc
     1381 gagcccggcg gcgacctgaa ggacggcaag cgcgcctgga cccaggacgt tccgctcggc
     1441 cgtttcgaga tgaagggtcc ggtgacctcg agcttctcgg tcggcggcca gtcgatcgcc
     1501 ctgaaccagg gcgagcagat cgccatccgc gctgcccaga ccaacgtcga cagcgtcagt
     1561 ctcgccggcc tgccggtggt cttcgccggc tatggcgtgc gggcgcccga gcgcaactgg
     1621 gacgacttcg ccggcatgga cgtcaagggc aaggtcgtga tcgtgctgat caacgacccc
     1681 gacttcgagt ccggcaaggg cgacttcggc ggcaaggcca tgacctacta cggccgctgg
     1741 acctacaagt acgaggaggc cgcgcgcctc ggggccgccg ccctgatcat cgtccacgag
     1801 acagacccgg cctcctacgg ctgggccacg gtgaagaact ccaacaccaa cacgatgttc
     1861 gacatcgtcc gcgacaagcc ggccgaggtt cacccgccgg tcgaggcctg gattcagcgc
     1921 gacgtggccg tcgatctctt ccgcagggcc ggtctggact tcgacgccct gaagaagaag
     1981 gccgccaagc gcggcttcaa ggccgtggcc ctgcccggcg tgaccttctc gaccgacttc
     2041 aaggtcgacc accaggtgat cgtgtcgcag aacgtcgccg gcgtgctgcc cggggccacg
     2101 cggcccgacg agcgggtgtt ctacaccgcc cactgggatc acctgggcgt gggcctgccc
     2161 gacgccagcg gcgacaccat ctacaatggc gccgtcgaca acgcgacggg cgtcgccgcc
     2221 ctgctcgagc tgggccggat gtacgccgcc gccccccgtc cggaccgctc ggtggtcttc
     2281 ctggccgtga cggccgagga aaagggcctg ctgggctccg aatactacgc cgccaatccg
     2341 ctctatccgc tggccacgac ggtcgccgat ctgaacatgg atggcttctc gccgaccggc
     2401 ccgtcgcgcg actacaccac ctcgggagac ggcaaggtca gcctgcagga tgatctggtg
     2461 gcggccctca aggcgcgcgg ccgggtgttc acgcccgacc ccgttccgga agccggccac
     2521 ttcttccgct cggaccactt cccgttcgcc aagcgcggcg tcccggccat ctcggtcggc
     2581 ccgggcgagg acctggtcgt cggcggcgtc gccgcgggca aggcctacaa cgacatgtac
     2641 atcaaggacc gctaccacca gccggccgac gaatggtcgg acgactggga cctgtcgggc
     2701 atggcgaccg acctgaccct gctctatgac ctgggccgcg acctcgccac ctcgacccgc
     2761 tggcccgaat ggcaggcggg gtccgagttc aaggcggtgc gcgacgccac cgcggctgaa
     2821 cgccagtagc cgctgatccg cgagccccgg cgcctgcgcc ggggtgatcg gagcgcacga
     2881 tgaccgtcag cgtcttcgac ctgttcaagc ccgggatcgg gccgtcgag
//
LOCUS       DAORNX010000374         2912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_25810_length_2912_cov_5.118656, whole genome shotgun sequence.
ACCESSION   DAORNX010000374 DAORNX010000000
VERSION     DAORNX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25810_length_2912_cov_5.118656"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLO65_13890"
     CDS             <1..220
                     /locus_tag="PLO65_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29386.1"
                     /translation="GLEPSHRWLVLAQVYAGQADAGPEGAPRWIKLEISAVQRFGDWR
                     LQTGWRQAVAGRSTPVEAGPVVALWRRF"
     gene            341..1444
                     /locus_tag="PLO65_13895"
     CDS             341..1444
                     /locus_tag="PLO65_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD29387.1"
                     /translation="MIRRHFFLIAAATFLGLMLVGGALKLASAKSEGKGGPGGPRAAA
                     VSQAVVVAHPFADSIEALGVAKGRESVVITSNTSELVTAVRFRDGQTVSRGQVLVELK
                     AGEEDAGLIEAQARLAQAERDYIRWKTLAEQGIAPKATAEQYRSALDTARAGVEAAKA
                     RKLDRVIRAPFSGVVGLTDVAPGALISPGAPIVTLDDLSMVRVDFEVPDRYVPVLAEG
                     APIQARADAWPSEAFQGRIARLDSRIDAATRAITARAEFPNPGRRIKPGMLMRVSIAH
                     GARQGLAVPEAAVQFEGDQAFVFVITPRADGFSARKQAVETGVTEGGFVEITTGLRAG
                     DRVVADGLNRIQDGQPVRVAGAPRAAGPARKAG"
     gene            1444..>2912
                     /locus_tag="PLO65_13900"
     CDS             1444..>2912
                     /locus_tag="PLO65_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD29388.1"
                     /translation="MLSDLSVKRPVFAAVAAIILCVIGLAAFLNIPIRELPSVDPPVV
                     SISTTYRGASAEIVEERITQVLERQVSGIQGIDRVSSSSRDGRSQINIVFKLDTDLET
                     AANDVRDAASRVADQLPEQADPPQIAKANADSAPIIIINMTSTTLNPLQLADYADRFL
                     VERLSTIPGVAAVRMAGPTYAMRIWLDADQMAARGVTVDDVEAALNSQNVELPAGALE
                     STARDFTIRVNRGYASPKDFVRLPIKIAGQAARSDAYVTRLGDIARVEEGAAEDRRVF
                     RGNGIDQIGLVLTRQSQANDLAIAKAARREVAAINQTLPEGTSMIIAVDSSQFTAEAI
                     REVWITMGISIALVAMVNLLFLGSWRAAVIPSVVAPICLLSTFIVLAPLGFSLNLLTL
                     LALVLSVGLVVDDAIVVVENIQRRVDEGEPPTVAATRGARQVFFAVIATTIVLISVFG
                     PLMFLPGYIGRLFVELAAAIASAVAFSAFLALSLSPMLAS"
BASE COUNT          399 a         1041 c         1018 g          454 t
ORIGIN      
        1 cggccttgag ccgtcacacc gctggctggt gctggcgcag gtctacgccg gtcaggcgga
       61 tgccgggccc gagggcgcgc cacgctggat caagctggag atttcagcgg tgcagcggtt
      121 cggcgactgg cgcctgcaga ccggatggcg acaggccgtg gccgggcgat cgactccggt
      181 cgaggcgggg ccggtggtgg cgctctggcg caggttctga ttgttgcggc gctgaaacag
      241 accgtaacgc gtccgtcatc gcaatcgggt ttacgcgtac ggcaatgccc ctatatgggt
      301 ctccagcagc cgccggcttc gccggcgtcg ggagttgttc gtgatccgcc gccatttctt
      361 cctcatcgcc gccgcgacct ttcttgggct gatgctggtg ggcggggcgc tgaagctggc
      421 gtcggcgaaa tccgagggca agggcggccc cggcggcccg cgcgccgccg ccgtgtccca
      481 ggccgtcgtc gtcgcgcatc ccttcgcgga cagtatcgag gcgctcggcg tggccaaggg
      541 ccgcgagtcg gtggtcatca cctccaacac cagcgagctg gtcaccgccg tccgcttccg
      601 cgacggccag accgtgtcgc gcggccaggt gctggtcgag ctcaaggcgg gtgaagaaga
      661 cgccggcctg atcgaggccc aggcgcggct ggcccaggcc gagcgcgact acatccgctg
      721 gaagaccctg gccgaacagg ggatcgcgcc gaaggccacg gccgagcagt accgttccgc
      781 gctcgacacg gcccgcgccg gggtcgaggc ggccaaggcg cgcaagctgg accgggtcat
      841 ccgggcgccc ttctccggcg tggtcggcct gaccgacgtg gccccgggcg ccctcatcag
      901 ccccggcgcg ccgatcgtca ccctcgacga tctgtcgatg gtgcgggtcg atttcgaggt
      961 gccggaccgc tacgtgccgg tgctggccga gggcgcgccg atccaggccc gcgccgacgc
     1021 ctggccgagc gaagccttcc agggccgcat cgcccggctg gacagccgca tcgacgcggc
     1081 gacccgcgcc atcaccgccc gcgccgagtt tcccaatccc ggacgacgca tcaagccggg
     1141 catgctgatg cgtgtgtcga tcgcccacgg cgcccgccag ggcctggccg tgcctgaggc
     1201 ggccgtgcag ttcgagggcg accaggcctt cgtgttcgtt atcaccccgc gcgccgatgg
     1261 cttctcggcc cgcaagcagg cggtcgagac cggcgtcacc gagggcggct tcgtcgagat
     1321 caccaccggc ctgcgggccg gcgaccgggt ggtcgccgac ggcctgaacc ggatccagga
     1381 tggtcagccg gtcagggtcg cgggcgcgcc gcgcgccgcc ggaccggccc ggaaggccgg
     1441 ctgatgctgt cggacctgtc ggtcaagcgg ccggtcttcg cggcggtcgc ggcgatcatc
     1501 ctctgcgtca tcggcctggc cgcgttcctg aacatcccga tccgcgagct gcccagcgtc
     1561 gacccgcccg tggtgtcgat ctcgaccacc tatcgtggcg cctcggccga gatcgtcgag
     1621 gagcggatca cccaggtgct cgagcgccag gtcagcggaa tccagggcat cgaccgggtg
     1681 tcctcgtcca gccgcgacgg ccgtagccag atcaacatcg tcttcaagct cgacaccgac
     1741 ctggagacgg cggccaacga cgtgcgcgac gccgccagcc gggtcgccga ccagctgccc
     1801 gaacaggccg atccccccca gatcgccaag gccaacgccg actccgcgcc gatcatcatc
     1861 atcaacatga cctcgacgac gctgaatccg cttcagctcg ccgactacgc cgaccgcttc
     1921 ctcgtcgagc ggctgtcgac cataccgggg gtggcggcgg tgcggatggc cgggccgacc
     1981 tacgccatgc gcatctggct cgacgcggac cagatggcgg cgcgcggcgt caccgtggat
     2041 gacgtcgagg cggccctcaa cagccagaac gtcgagcttc cggccggggc gctggagagc
     2101 accgcccgcg acttcaccat ccgcgtcaac cggggctacg cctcgccgaa ggacttcgtg
     2161 cggctgccga tcaagatcgc cggccaggcc gcgcgcagcg atgcctatgt cacccgcctg
     2221 ggcgacatcg cgcgggtcga ggagggggcg gccgaggacc ggcgcgtctt ccgcggcaac
     2281 ggcatcgacc agatcggcct ggtcctgacg cgccagtccc aggccaacga cctggccatc
     2341 gccaaggccg cccggcggga ggtggcggcg atcaaccaga ccctgcccga gggcacgtcg
     2401 atgatcatcg ccgtggattc ctcgcagttc accgccgagg caatccgcga ggtgtggatc
     2461 accatgggca tctcgatcgc cctggtggcg atggtcaacc tgctgttcct cggcagctgg
     2521 cgggcggcgg tgatcccgtc ggtggtggct cccatctgtc tgctgtcgac cttcatcgtg
     2581 ctggctccgc tgggcttttc gctgaacctg ctgaccctgc tggcgctggt gctgtcggtc
     2641 gggctggtgg tcgacgacgc catcgtggtg gtcgagaata tccagcggcg cgtggacgag
     2701 ggcgagccgc cgaccgtcgc cgccacccgc ggcgcgcggc aggtgttctt cgccgtgatc
     2761 gccaccacca tcgtgctgat ctcggtgttc gggccgctga tgttcctgcc cggctatatc
     2821 ggccggctgt ttgtcgagct ggcggccgcc atcgcctcgg cagtggcctt ctcggccttc
     2881 ctggccctgt ccctgtcgcc gatgctggcc tc
//
LOCUS       DAORNX010000375         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26289_length_2873_cov_9.233144, whole genome shotgun sequence.
ACCESSION   DAORNX010000375 DAORNX010000000
VERSION     DAORNX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26289_length_2873_cov_9.233144"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..694
                     /gene="fabA"
                     /locus_tag="PLO65_13905"
     CDS             194..694
                     /gene="fabA"
                     /locus_tag="PLO65_13905"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HRD29389.1"
                     /translation="MAQSSYSYDDLIASGRDETFGPGNAQLPMPPMLMFDRITNITSD
                     GGAHGKGLVEAELDIRPDLWFFACHFNNDPVMPGCLGLDAMWQLVGFYLGWIGGQGRG
                     RALGVGEVKFTGEVTPEIKKVVYRVELKRVINRRLVMGIGDGVLEADGKPIYEASDLR
                     VGLYAK"
     gene            760..1971
                     /gene="fabB"
                     /locus_tag="PLO65_13910"
     CDS             760..1971
                     /gene="fabB"
                     /locus_tag="PLO65_13910"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRD29390.1"
                     /translation="MRRVAVTGIGIVSSIGANANEVVASLREAKSGVKFAQSYADLGF
                     RSQVEAAPDCDWESIVDRRAARFLAEGTGWAHVAMEQAIADSGLEDSEVSNEHTGLIV
                     GSGGPSTRAIIAAAEITREKGPKRIGPFAVPKAMSSGPSATLATWFRIRGLNFSISSA
                     CATSTHCIGSAYEQILMGKQEVVFAGGTEELDWSLSNMFDAMGAMSSNFNDRPAVASR
                     AYDLNRDGFVIAGGAGIVVLEEMERARARGAKIYGEIVGYAANSDGHDMVAPSGEGAV
                     RCMRLAMAGAGGRTIDYLNPHGTSTPVGDLKEMEAVREVFGAKAPAISSTKSLTGHSL
                     GAAGAQEAIYSLLMLNNGFLAESANIETLDPAFEDLPILRKREDRQVETVMSNSFGFG
                     GTNGCLVMARA"
     gene            2218..>2873
                     /locus_tag="PLO65_13915"
     CDS             2218..>2873
                     /locus_tag="PLO65_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRD29391.1"
                     /translation="MADDYTFPKGELMKGKKALITGVANHNSIAWGIASQLAAQGAEL
                     AFAYMPDMERRVKPLGESIGVKTFVPMEVTDDASMDAAFAQIGKDMGTLDAFLHSIAF
                     ANKDELKGSFVENTTREGFLRAMNISAFSFVDCARRAAELMPNGGSMVTMTYLGAERV
                     IPNYNTMGVAKAALEAATRYIARDLGPRGIRANAISAGAMRTLSLAGISGGRGMISQG
                     R"
BASE COUNT          480 a          993 c          980 g          420 t
ORIGIN      
        1 actggcggcg gatcaaggcg tcgggcgagg ccggctgggt gccggccgcc gaggtctggg
       61 gcgccggcgg cccgacgccc tgccggccgc gcgaagattg aggccctctc ccccgcgtgg
      121 taacccgcgc cagtcaggcc gcgcggacgc ggcgaagttt cggtgacgca tgacctcgac
      181 gaacccgccg cccatggcgc agtccagcta cagctacgac gacctgatcg cctcgggccg
      241 cgacgagacc ttcggtcccg gcaacgcgca gctgcccatg ccgccgatgc tgatgttcga
      301 ccggatcacc aacatcacca gcgacggcgg tgcgcacggc aagggcctcg tcgaggccga
      361 gctcgatatc cgtcccgacc tgtggttctt cgcctgccac ttcaacaacg acccggtgat
      421 gcccggctgc ctcggcctgg acgccatgtg gcagctggtc ggcttctatc tcggctggat
      481 cggcggccag ggccgcggcc gcgccctcgg cgtgggcgag gtcaagttca ccggcgaggt
      541 gacgccggag atcaagaagg tcgtctaccg cgtcgaactc aagcgggtga tcaaccgccg
      601 gctcgttatg ggcataggcg acggggtgct ggaagccgac ggaaagccta tctatgaggc
      661 cagcgatctt cgcgtcggcc tttacgcgaa atagccggcg gagaaccgga cctgcttgga
      721 tccgcccccc gtggggcggg tggggagaga ggagtcatca tgcggcgagt ggccgtcacc
      781 ggcatcggaa tcgtgtcctc gatcggcgcc aacgccaatg aggtggtcgc ttcgcttcgt
      841 gaggcgaagt cgggcgtcaa gttcgcccag tcctatgccg acctcggttt ccgcagccag
      901 gtcgaggcgg ctcccgactg cgactgggag tccatcgtcg atcggcgggc cgcgcgcttt
      961 ctcgccgagg gcaccggctg ggcccacgtc gccatggagc aggcgatcgc cgactccggc
     1021 ctcgaggaca gcgaggtttc caacgagcac accggcctga tcgtcggctc gggcggtccc
     1081 tcgacgcggg ccatcatcgc cgccgccgag atcacccggg agaagggccc caagcggatc
     1141 gggccgttcg ccgtgcccaa ggccatgagc tcgggcccat cggcgaccct ggccacctgg
     1201 ttcaggattc gcggcctcaa cttctcgatc agctccgcct gcgccacctc gacccactgc
     1261 atcggctcgg cctacgagca gatcctgatg ggcaagcagg aggtggtctt cgccggcggc
     1321 accgaggaac tcgactggag cctgtcgaac atgttcgacg ccatgggcgc catgagcagc
     1381 aacttcaacg accggccggc cgtggccagc cgcgcctacg acctgaaccg cgacggcttc
     1441 gtgatcgctg gcggcgccgg catcgtggtg ctggaggaaa tggagcgggc ccgggcccgg
     1501 ggcgcgaaga tctacggcga gatcgtcggc tacgcggcca attccgatgg ccacgacatg
     1561 gtcgccccgt ccggcgaggg cgcggtccgc tgcatgcgcc tggccatggc cggcgccggc
     1621 ggccggacca tcgactacct caacccgcac ggcacctcga ccccggtcgg cgacctgaag
     1681 gagatggagg ccgtgcgcga ggtgttcggc gcgaaggccc cggccatttc ctcgaccaag
     1741 tcgctgaccg gccacagcct gggcgcggcc ggcgcgcagg aggcgatcta cagcctgctg
     1801 atgctgaaca acggcttcct cgccgagagc gccaacatcg agaccctcga tccggcgttc
     1861 gaggacctgc cgatcctgcg caagcgcgag gaccgccagg tcgagacggt gatgtccaac
     1921 agcttcggct tcggcggcac caatggctgc ctggtcatgg cgcgggcgta gcccaaatcc
     1981 ccgtcatccc cgcgctcgtc gcgaggacca ggcgtgttca acccggtcag aagggcgtgg
     2041 cgggatccgt gcccttcctt ccgtccatcg ggttgatggg tcgtcggaac aagtccgatg
     2101 atgacgaatg cagagggggc gcggcacttg ccccggcgca cgccgccgtc cagaaaggcg
     2161 cccgacagcc ggtcagagac gcggcgcgcg cacgaccgac caggacggag atcccacatg
     2221 gccgacgact acaccttccc caaaggggag ctgatgaagg gcaagaaagc cctgatcacc
     2281 ggggtggcga accacaactc gatcgcttgg ggcatcgcct cgcagctggc cgcccagggc
     2341 gccgagctgg ccttcgccta catgccggac atggagcggc gcgtgaagcc gctgggcgag
     2401 agcatcgggg tcaagacctt cgtgccgatg gaggtcacag acgacgcctc gatggacgcc
     2461 gccttcgccc agatcggcaa ggacatgggg acgctggacg ccttcctcca ctcgatcgcc
     2521 ttcgccaaca aggatgagct gaaaggctcg ttcgtcgaga acaccacccg cgagggcttc
     2581 ctgcgggcga tgaatatctc ggccttcagc ttcgtcgact gcgcccggcg cgcggccgag
     2641 ctgatgccca acggcgggtc gatggtgacc atgacctatc tcggggccga gcgggtcatt
     2701 cccaactaca acactatggg cgttgccaag gcggcgctcg aggcggcgac ccgctacatc
     2761 gcccgcgacc tgggacccag gggcatccgc gccaacgcca tctcggccgg ggccatgcgc
     2821 accctgtcgc tggccggcat ctccggcggc cgcggcatga tcagccaggg ccg
//
LOCUS       DAORNX010000376         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26435_length_2860_cov_8.081640, whole genome shotgun sequence.
ACCESSION   DAORNX010000376 DAORNX010000000
VERSION     DAORNX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26435_length_2860_cov_8.081640"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLO65_13920"
     CDS             <1..420
                     /locus_tag="PLO65_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRD29392.1"
                     /translation="PWRFIVLRGEAKATLADRLEAVAAARPDAPKLVAKLGKLKAPPI
                     GVAVVSRTVDGEIPAWEQLLSAGAVCTTLLYAATAMGYGANWITDWYAYDPEARAVLG
                     LSPDEQVAGFVFIGTAAEPPQERVRPDVTTLTTIWKP"
     gene            complement(417..872)
                     /gene="queF"
                     /locus_tag="PLO65_13925"
     CDS             complement(417..872)
                     /gene="queF"
                     /locus_tag="PLO65_13925"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRD29393.1"
                     /translation="MSELHLTQLGQAVDAPARPEDAVLERVPNPQADTLFLARFVAPE
                     FTSLCPVTGQPDFAHLVIDYAPADWLIESKSLKLYLGSFRNHGAFHEDCTVAIGRKIV
                     DVARPKWLRIGGYWYPRGGIPIDVFWQSGPAPEGLWVPDQGVAPYRGRG"
     gene            959..1762
                     /locus_tag="PLO65_13930"
     CDS             959..1762
                     /locus_tag="PLO65_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29394.1"
                     /translation="MAGKDFDGFVVLVTGGSTGLGRAIAVETASRGAAAVIINYASSN
                     AEAEETARLCEAEGARTVLVQGDVGDDADCRRIAAAAEPFGRIDALFNNAGITKFAPN
                     HGDLDAVDADDFLRLYRVNVVGAFQMVRAARALLEAAPQPGAVVNTASIAGVTGIGSS
                     VPYAASKGAMTTMTLSLARGLAPRIRVNAICPGFIDTPWFGKAMPDDRIERMRQGVIN
                     STPLQAASTAEDIAGAAVFLASPASRHVTGETLLADAGTHLGFASLSMR"
     gene            2035..2709
                     /locus_tag="PLO65_13935"
     CDS             2035..2709
                     /locus_tag="PLO65_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRD29395.1"
                     /translation="MKALKLALCAAAASFAMAGAASAQDISFNLAATTDYVFRGYSQT
                     DESPALQGGVDLSQGVLYGGLWASNVDFLDSTDAEVDAYVGIKPTAGAVTFDFAAIYY
                     GYVNAPSSASYDYWEFKAAASAPVGPATIGAAVYYSPAFFGDIGDATYYEVNGSFSPA
                     EKWSVSGALGEQTFADVAGDYTTWNVGVGYAITDSISADLRYHDSDVDSPISDERVAL
                     TLKLVK"
BASE COUNT          445 a         1011 c          966 g          438 t
ORIGIN      
        1 ccctggcgct tcatcgtgtt gcgcggcgag gccaaggcga ctctggccga ccggctcgag
       61 gcggtcgccg ccgcccgccc cgacgcgccc aaactggtcg ccaagctcgg caagctgaag
      121 gccccgccga tcggcgtggc cgtggtgtcc cgcacggtcg acggcgagat tcccgcctgg
      181 gagcagctgc tgtcagccgg cgcggtctgc accaccctcc tctacgcggc cacggccatg
      241 ggctacggcg ccaactggat caccgactgg tacgcctatg atcccgaagc gcgcgccgtg
      301 ctgggcctgt cgccggacga gcaggtggcc gggttcgtgt tcatcggcac cgccgccgag
      361 ccgccgcagg agcgggtgcg gccggacgtc acgacactga cgacgatctg gaagccctaa
      421 ccgcggccgc gatacggcgc gacgccctga tccggaaccc aaaggccctc cggcgccggg
      481 ccgctctgcc agaacacgtc gatcgggatg cccccgcgcg ggtaccagta tccgccgatg
      541 cgcagccact tcggccgggc gacgtcgacg atctttcggc cgatggcgac cgtgcagtcc
      601 tcgtggaagg cgccgtgatt gcggaaagag cccagataga gcttgagcga cttggactcg
      661 atgagccagt cggccggcgc gtagtcgatg accagatggg cgaaatcggg ctggcccgtc
      721 accgggcaca gcgaggtgaa ctccggcgcc acgaagcggg cgaggaacag cgtatcggcc
      781 tgcgggttgg gcacgcgttc gaggaccgcg tcctccggcc gcgcgggggc gtcgaccgcc
      841 tggcccagct gggtgaggtg aagctcgctc atggccagcg gctttaaacc ggcggtccgg
      901 gcgggacaag gtcggcgcgg cgcgctatca gaccggaaac aggctcgggg agacgcagat
      961 ggccggcaag gatttcgacg gtttcgtggt gctggtgacc ggcggctcga cgggcctggg
     1021 ccgggccata gcggttgaga ccgccagccg cggcgcggcg gccgtgatca tcaattacgc
     1081 ctccagcaac gccgaggccg aggagacggc ccgcctgtgc gaagccgaag gcgccaggac
     1141 cgtgctggtc cagggcgatg tcggcgacga cgccgactgt cgccggatcg cggccgccgc
     1201 cgaaccgttc ggccgcatcg acgccctgtt caacaacgcg ggcatcacca agttcgcgcc
     1261 caatcacggc gacctggacg cggtcgatgc cgacgacttc ctgcgcctct accgggtcaa
     1321 tgtcgtgggc gccttccaga tggtgcgggc ggcgcgcgcc ctgctcgagg cggccccgca
     1381 gccgggcgcg gtggtcaaca ccgcctcgat cgccggcgtt accggcatcg gctcgtcggt
     1441 gccgtacgct gcgtccaagg gcgcgatgac gaccatgacc ctgtcgctgg cccggggcct
     1501 ggcgcccagg atccgcgtca acgccatctg cccgggcttc atcgacacgc cctggttcgg
     1561 caaggccatg cccgacgacc gcatcgagcg catgcgccag ggcgtgatca actccacgcc
     1621 gctgcaggcc gcctcgaccg ccgaggacat cgccggcgcc gccgtgttcc tcgcctcccc
     1681 ggcctcgcgt cacgtcaccg gcgagaccct tctggccgac gcgggcacgc atctgggctt
     1741 cgcctcgctg tcgatgcgct gatccttacg gcccccttac gtccggttcc ggcatttcgc
     1801 attgtggtga aattcccgga ttgagaggct gtcggcgccc ataaaacggg cggatgacgg
     1861 ttgcagcgca caatttgccg ccagccaaac gcctgttggg ccgatccgcc gcgccaccgg
     1921 taggggcgcg gagacgcttc gaccactctc aacctcgctc atcgccgtgc ggcggggcga
     1981 cgctggcttc acgtcaaaaa cgcgggggcc ggcgccaaca aaaaggggat agcgatgaaa
     2041 gctcttaaac ttgctctttg cgccgccgcg gcgtccttcg ccatggccgg ggcggcttct
     2101 gcccaggaca tctcgttcaa cctggcggcc acgaccgact atgtgttccg cggctacagc
     2161 cagaccgacg agagtcccgc cctccagggc ggcgtggatc tgtcccaggg cgttctctac
     2221 ggcggtctgt gggcgtccaa cgtggacttc ctcgacagca ccgacgccga ggtcgacgcc
     2281 tatgtcggca tcaagccgac ggccggcgcg gtgaccttcg acttcgcggc gatctactac
     2341 ggctacgtca acgcgccctc gagcgcctcg tatgactact gggaattcaa ggccgccgcc
     2401 tcggcgccgg tcggtccggc caccatcggc gcggccgtct actattcgcc ggccttcttc
     2461 ggcgacatcg gcgacgcgac ctattacgag gtcaatggct cgttctcgcc ggccgagaag
     2521 tggagcgtgt ccggcgccct gggcgaacag accttcgccg acgtcgccgg cgactacacc
     2581 acctggaacg tcggcgtcgg ctacgccatc accgattcca tttccgccga cctgcgctac
     2641 cacgacagcg acgtcgactc gccgatttcc gacgagcgcg tggccctgac gctcaagctg
     2701 gtgaagtagc cggccccgac cacctcctct ccggggtgat cgtctggaag ccgtcgcggc
     2761 ccgccgcggc ggcttttcca tgccgggcga aggacgaggg tggtggggac tgtgggagtc
     2821 gaacccacac gggccgcaag gcccaacgga ttttaagtcc
//
LOCUS       DAORNX010000377         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26557_length_2849_cov_5.895132, whole genome shotgun sequence.
ACCESSION   DAORNX010000377 DAORNX010000000
VERSION     DAORNX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26557_length_2849_cov_5.895132"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLO65_13940"
     CDS             <1..240
                     /locus_tag="PLO65_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29396.1"
                     /translation="RDGRPYSFVRKVPEGEPATPAEGYRLVLEGRVTGYPGGHAVRCR
                     SDSPDRRPVCLVAVEFDRVAFEDGATGAVLAEWRE"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(298..690)
                     /locus_tag="PLO65_13945"
     CDS             complement(298..690)
                     /locus_tag="PLO65_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD29397.1"
                     /translation="MIRDLDHINIATPLLSETRDFWRDVMGLTERWRPDFPFDGHWLY
                     AGDRPVVHLMGRPAQGVGSDQATLNHVAFAIADLEAARARLTRLGVAFREIGVPGSPI
                     LQLFLQDPNGITIELNYLGAETAKARST"
     gene            complement(693..2651)
                     /gene="rnr"
                     /locus_tag="PLO65_13950"
                     /pseudo
     CDS             complement(693..2651)
                     /gene="rnr"
                     /locus_tag="PLO65_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
BASE COUNT          391 a          965 c         1012 g          471 t
ORIGIN      
        1 cgggacggcc gcccctattc gttcgtgcgc aaggtcccgg agggagagcc ggcgacgccg
       61 gccgagggct atcgcctggt gcttgagggc cgcgtcaccg gctacccggg cggtcacgcg
      121 gtgcgctgcc gcagcgactc gcccgaccgg cggccggtct gcctcgtggc ggtcgagttc
      181 gaccgcgtcg ccttcgagga cggcgcgacg ggggcagtgc tggcggagtg gcgggaatag
      241 tcgacgcggt cgaactcnnn nnnnnnngtc gacgcagagg ccccctggcg gcaagcctca
      301 ggtcgagcgc gccttcgcgg tctcggcgcc cagatagttc agctcgatcg tgatcccgtt
      361 ggggtcctgc aggaacagct ggaggatcgg tgagccgggc acgccaatct cgcggaacgc
      421 cacgcccagc cgcgtgagcc gcgcccgggc cgcctcgagg tcggcgatgg cgaaggccac
      481 atgattgagc gtggcctggt cggacccgac cccctgagcc ggccggccca tcagatgcac
      541 gaccggcctg tcgcccgcat agagccagtg tccgtcaaac ggaaagtccg gccgccaccg
      601 ctcggtcagc cccatcacat cgcgccagaa gtcccgcgtc tcggagagca gcggcgtggc
      661 gatgttgatg tggtcgagat cgcggatcat ggctaccgcc gcttgccctt gcgcacgccc
      721 ttcggcggtc cgccgcgctt gggcccgccg cggcccggat ccatccgccg cctgacgccc
      781 agccgtggcc ggggcgcgtt cggatcggcc ggcatcggct cgctgagcat ctcgaacagc
      841 agaccgcccg tcaccggcgt cgcctcgcgc aggcggacct cgaccggcat gcccagcggc
      901 cagcgcgcgc cgctgcgctc gccgaccagg gcgtgggtcc ggtcgtcatg gatgaagaac
      961 tcgtcgccca ggcccgagat cgggatcagc ccgtcggcgc ccgtgtcggt caggcggatg
     1021 aacaggccga accgggtcac cccggtgatg cggccgtgga agtcgctgcc gacctggtcg
     1081 gccaggaagg cggcgacgta gcggtccgtc gcgtcgcgct cggcggccat ggcgcggcgc
     1141 tcggcgaagg tgatccgctc ggcggtgtcc ttcatctgct tgacgtcctg gtcggtcagg
     1201 ccgtcggtcc ccaggcccag ggcgcggatc agaccgcggt gcaccaccag gtcggcgtag
     1261 cggcggatcg ggctggtgaa gtgggcgtag cgctgcaggt tcaggccgaa gtggccgatg
     1321 ttgtccgggt tgtactgcgc ctgcatctgg gtgcgcagga ccacctcgtt gacgatgtcg
     1381 gcgtgcggcc cgtcgcgggt ctcctccagc aggcggttga accggtccgt tcgcggcgcc
     1441 tcgcccttgg accaggggat gtccagggtc gccaggaagt cggtcagcgc catcatcttc
     1501 tccggcgatg gcgtgtcgtg gatgcgatag atcagcggcg tcttcttctg ctccagcgtc
     1561 tcggcggcgc agacgttggc ctggaccatc atctcctcga tcagccggtg ggcctcgagc
     1621 gacgtgcgcc tggtgatcga ggcgacatgg ccctcgggcg agatgacgat gcgccgctcg
     1681 tcgctctcga tggccagcgg cgagcgggcg aggcggccct tgagcatcac gcggtaggcc
     1741 gtccacagcg gcgtcaggat gccgtccagg atggcgccgg ccttgtcgtc cggattgccg
     1801 tcgatggcca tctgggcctg ctcatagctg agcttggccg ccgagcgcat caggccgcgc
     1861 acgaagcggt ggctgatctt gcggccgtcc ttgctgaaca ccatccgcac ggccagggtc
     1921 gcgcggttct cgccctcgcg caggctgcac aggccattgc tcagccgttc cggcagcatc
     1981 ggctcgaccc ggtcggggaa gtagacgctg ttggacttgt cgcgcgcgat ggcgtcgagc
     2041 gccgagccgg gccgcacgta ggcggcgaca tcggcgatgg ccacccagac gatccagccg
     2101 ccgacgttgc gcgcgtccgg atcgggatgg gcgaacaccg cgtcgtcatg atcccgcgcg
     2161 tcggccgggt cgatggtgat gaatggcagg tcgcgcagat cctcgcgccc ctgcaaggtc
     2221 ggggcctggg ccgcctcggc ctcggcttcg gcctcgggtg agaaaccggt cgggatgccg
     2281 tggctgtgga tggcgatcag cgaggccgcg cgcggctcgt cctcgcgccc gaccatctcg
     2341 accagcttgc cgtgtttggg gccgtagcgc tgggtctggg cgccgacggt cgccagcacc
     2401 aggtcgccgt ccttcagcct ttcgccgtcg gcgaacgaca ggaccaggct gtccttggac
     2461 ttgcggtcga cgggctcgac gcggatttcg cggtgggact tgcggatgac gcccaggatg
     2521 cggtgggcgc tctggcccag ccttttgatc agccgggcct cgtattcgcc ggtctcgagc
     2581 cggtcgaagt ggaccagcac ccggtcgccc aggcccggcg cgccggccac ggcctcgccc
     2641 cgggccggcg cctcgcccgg ttcgcaatta tcgggcggca ctatggctaa aatatccaga
     2701 tccagccgct ggttggcttt gctcaattcc gtggcaatgg tcacattcag acattcctca
     2761 ggggctatgg tctccacctg aaacgtcaac tccgtatggg ttggatacaa gtcctcccac
     2821 agatcgatgt aaagatcttc atcaatgcc
//
LOCUS       DAORNX010000378         2838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26692_length_2838_cov_7.423644, whole genome shotgun sequence.
ACCESSION   DAORNX010000378 DAORNX010000000
VERSION     DAORNX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26692_length_2838_cov_7.423644"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1535
                     /locus_tag="PLO65_13955"
     CDS             240..1535
                     /locus_tag="PLO65_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRD29398.1"
                     /translation="MTLPQLISLAVLIGVVSALIWGKLRSDVVALAGAAVLLGAGAIR
                     PVEVQGAFASPAIIALASLFVIAYAMELSGLLGLLIRKATDLCARFGAAGLWLVVVIS
                     GAASALLNNTPIVVLAGPVVREVAESMNLSPKRFLIPLSYATILGGACTLIGTSTNLL
                     VNDMARNAGQPVFGMFEITPVGLIIAAAGGLYLFLFSGRLLLKGESAEAEAPLPARAA
                     EASGDDPALFPVDRPFKPVESMAALAVFCGVVVSAAIGLAPIAAAAFFGAVLLVLLRV
                     ISVEDAYRGLRPETLLLIAGMVVVGLSLEVTGLASLATSALVDWVRPLGPLFALALLY
                     GGTLLMTEILSNAAVAVLLTPVAVALAESLGVSPRPFLVCLMMAASAAFATPFGYQTN
                     VLVFQLGKYSYMDFVRIGVPLNLLTWLVGVAAIPVFFPF"
     gene            1612..2625
                     /locus_tag="PLO65_13960"
     CDS             1612..2625
                     /locus_tag="PLO65_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 domain-containing protein"
                     /protein_id="HRD29399.1"
                     /translation="MKSTALAVIAALLAVPALANDSTAELAAGGLVLTRTEAIEMRSE
                     DLYISEQAVRVRYVFINTSAAPVTTRVAFPMPDIGGEEFFYSDNSIPNREDPANLLDF
                     RTTVDGRAVTMAVEQKAVARGVDRTDWLRGHGIPLALHLDGIVAKLDALPQAVKDEAL
                     ALGLVTRDEYDAGKGWESHLSPAWVLKTTFHWEQTFAPGAEVAVEHSYRPAVGGSVGT
                     MVGSPGYMADAEGAAERARYCIDSAFLGAVERAKKGRDYPPFTETRIAYVLKTGGNWA
                     RPIGDFRMVIDKGAAANLVSFCASGVKKIGPTRFEVRKTNWRPDRDLSILILKPLPPE
                     AAE"
BASE COUNT          386 a          997 c          964 g          491 t
ORIGIN      
        1 gcggtcgccg gtttcaggct tgagaaacat cgcgcccagg atcagcaggg ccacgaccgc
       61 gaggatcgcc agcggcgcga tcagtgtgag cttgcggtcg gatggtcgct gactattgtc
      121 cggcatggcc ccaccatagc agatcgttgt ctccgggggt cgcccggccc gtccagttcc
      181 cgtttacttc ggaaagcgtc gcacttgtcc ggaaatctcc gccgccacgg ccggcttcga
      241 tgactctgcc acaactcatc tccctggcgg ttctgatcgg cgtggtgagc gcgctgatct
      301 ggggaaaact gcgctccgac gtcgtcgccc tcgccggggc ggcggttttg ctgggcgccg
      361 gcgccatccg tccggtcgag gtgcagggcg ccttcgccag tcccgcgatc atcgccctcg
      421 cctcgttgtt cgtgatcgcc tacgccatgg agttgtccgg attgctggga ctgctgatcc
      481 gcaaggcgac agacctctgc gcccggttcg gcgccgccgg cctgtggctg gtggtggtga
      541 tctccggcgc cgcctcggcc ctgctcaaca acaccccgat cgtcgtgctg gccggtccgg
      601 tcgtgcgcga ggtcgccgag tcgatgaacc tgtcgcccaa gcggttcctg atcccgctgt
      661 cctacgcaac gatcctgggc ggggcctgca cgctgatcgg cacctcgacc aacctgctgg
      721 tcaacgacat ggcccgcaac gccggccagc cggtgttcgg catgttcgag atcacgccgg
      781 tcggcctgat catcgccgcg gccggcggcc tgtacctgtt cctgttcagc ggccggctgc
      841 tgttgaaggg cgaatcggcc gaggccgagg cgccgctgcc agccagggcg gccgaggcct
      901 cgggcgatga tccggcgctg tttccggtcg atcggccgtt caagccggtc gagtcgatgg
      961 cggccctggc ggtgttctgc ggcgtggtgg tctccgccgc catcggcctc gccccgatcg
     1021 ccgccgccgc cttcttcggg gccgtgttgc tggtgttgct gcgggtcatc agcgtcgagg
     1081 acgcctatcg cggcctccgt ccggagacgc tgctgctgat cgccggcatg gtggtggtcg
     1141 gcctgtcgct ggaggtcacg ggcctggcgt cgctggcgac ctcggccctg gtggactggg
     1201 ttcggccgct cggaccgctg ttcgccctcg ccctgctcta cggcggcacc ctgctgatga
     1261 ccgagatcct gtcgaacgcg gcggtcgccg tgctactgac cccggtggcg gtggcgttgg
     1321 ccgagagcct gggcgtcagc ccgcgcccgt ttctcgtctg cctgatgatg gcggccagcg
     1381 cggccttcgc gacgccgttc ggctaccaga ccaacgtcct ggtcttccag ctcggcaagt
     1441 acagctacat ggacttcgtg cgtatcgggg tgccgctgaa cctgctgacc tggctggtcg
     1501 gggtggcggc cattccggtt ttcttcccct tctaggccgg tcgcctcgct ggcgaagcgt
     1561 gcggcccgat cccgtcgctg tgctagcctc gcacgccccg gaggccacgc catgaaatcc
     1621 accgccctcg ccgtcatcgc cgccctgctg gcggttcccg ccctggccaa tgattcgacg
     1681 gccgaactgg ccgccggcgg gctggtgctg accaggaccg aggccatcga gatgcgctcg
     1741 gaggacctgt acatctctga gcaggcggtc cgggtccgct acgtgttcat caacacctcg
     1801 gcggcgccgg tgaccacgcg ggtggccttc ccgatgccgg acatcggcgg ggaggagttc
     1861 ttctattccg acaactccat ccccaatcgc gaagaccccg ccaacctgct ggacttccgc
     1921 accacggtgg acggcagagc ggtgacgatg gctgtcgagc agaaggccgt ggcccgcggc
     1981 gtcgaccgca cagactggct gcgcggacac ggcattccgc tggccctgca cctcgacggg
     2041 atcgtcgcca agctcgacgc cctgccccag gcggtcaagg acgaggccct ggcgctgggg
     2101 ctggtcacgc gggacgaata cgatgccggc aagggctggg agtcgcacct cagccccgca
     2161 tgggtgctca agaccacctt ccattgggag cagaccttcg cgcccggcgc cgaggtggcg
     2221 gtcgagcaca gctaccggcc ggccgtcggc ggcagtgtcg gcacgatggt cggctcgcca
     2281 ggctacatgg ccgacgccga gggcgccgcc gaacgcgcgc gctactgcat cgattccgcc
     2341 tttctcggcg ccgtggagcg ggcgaagaag ggtcgcgact acccgccctt caccgagacg
     2401 cgcatcgcct atgtgctgaa gacgggcgga aactgggcca ggccgatcgg cgacttccgg
     2461 atggtcatcg acaagggcgc ggcggccaat ctggtcagct tctgcgccag cggtgttaag
     2521 aaaatcggcc cgacgcggtt cgaggtgcgc aagacgaact ggcgaccgga ccgcgacctg
     2581 tcgatcctga tcctcaagcc gctgccgccc gaggcggccg aatgacgcca cgtcatactt
     2641 ttcccgcgat ctgaggggcc tatcctgcgc ccatgaccgg acccgttctc gaccccagct
     2701 acgacccgcg cagcgacggc gccctgcgcg aggccggcga gcggacggtc aggccgcgcc
     2761 acgccgcgac cctgatcatc gtccggcgcg acgcgaggaa gccgcgcctg ctgatgggcc
     2821 ggcgcaatcg cggccacg
//
LOCUS       DAORNX010000379         2834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26737_length_2834_cov_7.465635, whole genome shotgun sequence.
ACCESSION   DAORNX010000379 DAORNX010000000
VERSION     DAORNX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26737_length_2834_cov_7.465635"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLO65_13965"
     CDS             <1..614
                     /locus_tag="PLO65_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRD29400.1"
                     /translation="LSLVALILVAAPVPLRRLGVARADDLWIALRWGVVLAVTTASFA
                     VIYRFGPSRRPAKWRWVAWGALAAALAWLGGSLGFSWYVNHVAHFDATYGPLGAVVAF
                     MTWVWFSVLALLAGAELNAEIEHQTAVDSTIGEDRPMGQRGAAMADTVGKPFILHESL
                     SHGAGMARRQARALLRPLRDGVSRSYPRASRAARPGPRRSGDR"
     gene            complement(547..1623)
                     /gene="prfA"
                     /locus_tag="PLO65_13970"
     CDS             complement(547..1623)
                     /gene="prfA"
                     /locus_tag="PLO65_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRD29401.1"
                     /translation="MRLPQARLDQVLDRFREIEARMGAATEGPEIVRLSKEHAELKPV
                     ADAVLALEKVLAEKPELEAMAADADPEISELARDELERLEARLPDMERDVALLLAPRD
                     KDENASAILEVRAGTGGDEAALFAGDLFRMYSRYAATRGWRVEIDSVSEGEMGGYKEI
                     IASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEDVDIVINDAD
                     IRIDTYRSSGAGGQHVNKTDSAVRITHLPTGVVVTSSEKSQHENRRKAMANLKARLYD
                     MQRQALDDARSDSRKSQVGSGDRSERIRTYNYPQGRVTDHRINLTLYNLPKIMEGEAL
                     DDVIDPLIAEDQAARLATLEDSFG"
     gene            complement(1696..>2834)
                     /locus_tag="PLO65_13975"
     CDS             complement(1696..>2834)
                     /locus_tag="PLO65_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRD29402.1"
                     /translation="AVRVLAQAMDTVNIRAGMDFVASVARPQDRRGPARLIGLSMRTG
                     AASTLTVSRTFDGALRLRELEEKVSPETVAACGRMQGSFHESANAIGANSAVIAEASK
                     LFAHKIDFSRDIKAGDPFCLVFDRKVTESGRTIEAGGLQYAEVKGQRFYGFTRADGKR
                     TFLDSIGKSIKGFLLRAPVDGARITSRFGLRRHPILGYTKMHQGIDFGVGTGTPILAA
                     GDGVVVECRRWGGYGNWVRIRHAGGYETGYGHLSRFGKSCKPGMRVSQGQVIAYSGST
                     GASTGPHLHYEVWLKGRRVNPMGAKIPAGNSVDPRDVANFNATKKRIETLIAPRRAAL
                     GIPAPTTPAKGAAVAAAPEESGPTKKLARTDFTAPRAGLKPRTG"
BASE COUNT          360 a          948 c         1057 g          469 t
ORIGIN      
        1 tcctgtccct ggtggccctg atcctggtcg ccgcgcccgt ccccctccgc cggctcggcg
       61 tggcgcgggc cgacgacctg tggatcgccc tgcgctgggg cgtggtgctg gcggtgacca
      121 cggccagctt cgcggtgatc taccgcttcg gccccagccg acggccggcc aaatggcggt
      181 gggtcgcctg gggcgcgctg gccgccgccc tggcctggct cggcggatcg ctgggcttct
      241 cctggtacgt caaccatgtg gctcacttcg acgccaccta cggaccgctc ggggcggtcg
      301 tcgctttcat gacctgggtc tggttctcgg tgctggccct gctcgcgggc gcggagctca
      361 acgccgagat cgaacaccag acggcggtcg actcgaccat cggcgaggac cgcccgatgg
      421 gtcagcgcgg agcggccatg gccgacaccg tcggcaagcc gttcatcctg cacgagagtc
      481 tcagccacgg cgccggaatg gcccggcggc aggcccgcgc cttgctccgg ccgctgcgcg
      541 acggcgtcag ccgaagctat cctcgagcgt cgcgagccgc gcggcctggt cctcggcgat
      601 caggggatcg atgacgtcgt cgagcgcctc gccctccatg atcttgggca ggttgtagag
      661 ggtcaggttg atgcggtggt cggtgacccg gccctgcggg tagttgtagg tgcggatgcg
      721 ctcggagcgg tcgccgctgc cgacctggct cttgcggctg tcggagcggg cgtcatcgag
      781 cgcctggcgc tgcatgtcgt agagccgcgc cttgaggttg gccatcgcct tgcggcggtt
      841 ctcgtgctgg ctcttttccg aggaggtcac gaccacgccg gtgggcaggt gggtgatgcg
      901 cacggccgag tcggtcttgt tgacgtgctg gccgccggcg ccgctggagc gataggtgtc
      961 gatgcggatg tcggcgtcat tgatcacgat gtcgacgtcc tcggcctcgg gcaggacggc
     1021 gacggtggcc gcggaggtgt ggatgcggcc ctgggcttcg gtcgccggca cccgctggac
     1081 ccggtgcacc ccgctctcga acttcagtcg gccgaacacc ccctcgccgg tgatggaggc
     1141 gatgatctcc ttgtagccgc ccatctcgcc ttcggagacg ctgtcgatct cgacgcgcca
     1201 gccgcgcgtg gcggcgtagc ggctgtacat gcggaacagg tcgccggcga acagggccgc
     1261 ctcgtcgccg ccggtgccgg cgcgcacctc gaggatcgcc gaagcgttct cgtccttgtc
     1321 gcgcggggcc agcagcaggg ccacgtcgcg ctccatgtcc ggcagtcggg cctcaagccg
     1381 ttccagttcg tcgcgggcga gttcggagat ttcgggatcg gcgtcggcgg ccatcgcctc
     1441 gagctcgggc ttctcggcga gcaccttttc cagcgccaga acggcatcgg ccaccggctt
     1501 cagctcggca tgctccttcg acaggcggac gatctcgggc ccctcggtgg ccgcgcccat
     1561 gcgcgcctcg atctcgcgga aacggtcgag cacctgatcg aggcgggcct ggggaagacg
     1621 caagggagac tatccggaag cagcggaggg aagggggcgc tgtttacggc gccgggccgc
     1681 gtcctgtcga caacctcagc cggtgcgggg cttgagaccg gcgcgcgggg cggtgaagtc
     1741 ggtacgggcc agcttcttgg tcgggcccga ctcctccggc gcggcggcga cggcggcgcc
     1801 cttggccggg gtcgtcgggg ccgggatgcc gagggcggcg cggcggggag cgatcagcgt
     1861 ctcgatgcgc ttcttggtgg cgttgaagtt ggccacgtcc cgcggatcga cgctgttccc
     1921 ggccgggatc ttggcgccca tcggattgac gcggcggccc ttgagccaga cctcatagtg
     1981 caggtgcggg ccggtcgagg cgccggtcga accggaatag gcgatcacct ggccctgact
     2041 gacccgcatg cccggcttgc agctcttgcc gaagcgggag aggtggccgt agccggtctc
     2101 gtagccgccg gcgtgacgga tgcgcaccca gttgccgtat ccgccccacc ggcggcattc
     2161 gacgacgacg ccgtcgccgg cggcgagaat cggcgtgccc gtgccgacac cgaagtcgat
     2221 gccctggtgc atcttggtgt agccgaggat cgggtgccgg cgcaggccga agcgcgaggt
     2281 gatgcgggct ccgtcgaccg gcgcgcgcag caggaagccc ttgatgctct tgccgatgga
     2341 atcgaggaag gtgcgcttcc cgtcggcccg ggtgaagccg tagaagcgct ggcccttgac
     2401 ctcggcgtac tgcaggccgc cggcctcgat ggtgcggccg ctctcggtga ccttgcggtc
     2461 gaacaccagg cagaacggat cgcccgcctt gatgtcgcgc gagaagtcga tcttgtgggc
     2521 gaacagcttg gaggcctcgg cgatgaccgc cgaattggcc ccgatggcgt tggcgctttc
     2581 gtggaacgag ccctgcatcc ggccgcaggc ggcgaccgtc tcggggctga ccttttcctc
     2641 cagctcgcgc aggcgaaggg cgccgtcgaa ggtgcgggag accgtcaggg tgctggcggc
     2701 gccggtgcgc atcgacaggc cgatcagccg ggccggcccg cggcggtcct gcggccgggc
     2761 caccgaggcg acgaagtcca tgccggcgcg gatgttcacc gtgtccatgg cctgggccag
     2821 cacgcggacg gcca
//
LOCUS       DAORNX010000380         2825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_26837_length_2825_cov_8.927437, whole genome shotgun sequence.
ACCESSION   DAORNX010000380 DAORNX010000000
VERSION     DAORNX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26837_length_2825_cov_8.927437"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2661
                     /locus_tag="PLO65_13980"
     CDS             <1..2661
                     /locus_tag="PLO65_13980"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRD29403.1"
                     /translation="EARAPAAPAADVRAATLDSLRAIMMIRAYRMRGHLRANLDPLGI
                     AMPEGDATELDPTTYGFTEADYDRPIFLDFVLGLETSTLREILEIVRRTYCGAVGVQY
                     MHISNPQEKGWLQERIEGRDKEINFTREGKIAILKKLIEAEGFEKFLHRRFPGTKRFG
                     LDGGEACIPALEQIIKRGGALGVKDIVLGMPHRGRLNVLAAVMGKPYHVIFHEFQGGS
                     SMPSDIEGSGDVKYHMGASSDRTFDDNSVHLSLTANPSHLEIVNPVVLGKARAKQAFT
                     LRETPDAGRAHVLPLLLHGDAAFAGQGVVAECLTLSGLKGFRTGGTVHFIVNNQIGFT
                     TDPRYSRTSPYPSDVALMVEAPIFHANGDDPEAVVFAAKVATEYRQKFGKDVVVDMFC
                     YRRFGHNEGDDPTMTQPLMYAKIKDHPSTRELYSRRLIAEGVATEADIAGWVSEFDAF
                     LDAEFEAGKTYKANKADWLDGKWAGLALPGDEERRGSTGVPLQKLVDLGRAITTIPER
                     IDAHRTVRRVIEGRRAAIDAGEGLDWGTAEHLAFATLLDEGFPVRLSGQDSVRGTFTQ
                     RHCDLIDQTTEEHYTFLNHVRPGQANFEVIDSALSEEAVLGFEYGFSLADPNTLTLWE
                     AQFGDFVNGAQVVIDQFISSGERKWLRMSGLVMLLPHGYEGQGPEHSSARLERFLQSC
                     AEDNMQVLNCSTPANYFHALRRQMHREFRKPLIVMTPKSLLRHKKAVSNLADLAEGSS
                     FHRVMVDGAEAGCDVGGITLKPDDQITRVIACSGKVYFDLIDARAKAGRDDVYIVRLE
                     QFYPWPMKSVMQVMGRFANAELVWCQEEPKNMGGWTFVDPWLELTLDRMSIKARRARY
                     VGRPASASTAAGLMSRHLKELETFLAEAFA"
     gene            2702..>2825
                     /locus_tag="PLO65_13985"
     CDS             2702..>2825
                     /locus_tag="PLO65_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HRD29404.1"
                     /translation="MADIMTPALGESVTEATVARWTKKPGDAVRKDEILVELETD"
BASE COUNT          492 a         1009 c          909 g          415 t
ORIGIN      
        1 gaggcccgcg ccccggccgc ccccgccgcc gacgtccggg ccgcgacgct cgattcgctg
       61 cgcgccatca tgatgatccg ggcctaccgg atgcgcggcc acctgcgcgc caacctcgac
      121 ccgctgggca tcgccatgcc cgagggcgac gccaccgagc tggacccgac gacctacggc
      181 ttcaccgagg ccgactacga ccgcccgatc ttcctcgact tcgtgctcgg gctggaaacc
      241 tcgacgctgc gcgagatcct cgagatcgtc cgccgcacct attgcggcgc ggtcggcgtg
      301 cagtacatgc acatctccaa tccgcaggag aagggctggc tgcaggagcg catcgagggg
      361 cgcgacaagg agatcaactt cacccgggaa ggcaagatcg ccatcctcaa gaagctgatc
      421 gaggccgagg gcttcgagaa attcctgcat cgccgcttcc ccggcaccaa gcgcttcggc
      481 ctcgacggcg gcgaggcctg catcccggcg ctggaacaga tcatcaagcg cggcggcgcg
      541 ttgggcgtga aggacatcgt cctgggcatg ccccaccgtg gccgcctgaa cgtgctggcc
      601 gccgtgatgg gcaagcccta ccacgtgatc ttccacgagt ttcagggcgg ctcgtcgatg
      661 ccgtcggaca tcgagggctc gggcgacgtc aagtatcaca tgggcgcctc gtcggaccgg
      721 acgttcgacg acaacagcgt ccacctgtcg ctgaccgcca atcccagcca cctcgagatc
      781 gtcaatccgg tggtgctggg caaggcgagg gccaagcagg ccttcaccct gcgcgagacg
      841 ccggacgccg gccgggcgca cgtcctgccg ctgctgctgc atggcgacgc ggccttcgcc
      901 ggtcagggcg tggtggccga gtgtctcacc ctctcgggcc tcaagggctt ccgcaccggc
      961 ggcaccgtcc atttcatcgt caacaaccag atcggcttca ccaccgaccc gcgctactcg
     1021 cgcaccagcc cctatccgtc ggacgtggcg ctgatggtcg aggcgccgat cttccacgcc
     1081 aacggcgacg atcccgaggc cgtggtcttc gccgccaagg tggccaccga gtaccgccag
     1141 aagttcggca aggacgtggt cgtcgacatg ttctgctacc gtcggttcgg tcacaacgag
     1201 ggtgacgacc cgacgatgac ccagcccttg atgtacgcga agatcaagga ccacccgtcg
     1261 acccgcgaac tgtattcccg ccggctgatc gccgagggcg tggcgaccga ggccgacatc
     1321 gccggctggg tcagcgagtt cgacgccttc ctcgacgccg agttcgaggc cggcaagacc
     1381 tacaaggcca acaaggccga ctggctggac ggcaagtggg ccggtctcgc cctgcccggc
     1441 gacgaggagc gccgcggcag caccggggtg ccgctccaga aactggtcga tcttggccgc
     1501 gccatcacca ccattcccga gcgcatcgac gcccacagga cggtccgccg ggtgatcgag
     1561 ggccgtcgcg ccgcgatcga cgcgggcgag gggctcgact ggggcacggc cgagcacctg
     1621 gccttcgcca ccctgctcga cgagggtttc cccgtgcgcc tcagcggcca ggacagcgtg
     1681 cgcggcacct tcacccagcg ccactgcgac ctgatcgacc agaccaccga ggaacactac
     1741 accttcctca accatgtccg gccggggcag gccaacttcg aggtcatcga ctcggcgctg
     1801 tccgaggagg cggtgctcgg cttcgagtac ggcttcagtc tcgccgaccc gaatacgctg
     1861 accctgtggg aagcccagtt cggcgacttc gtgaacggcg cccaggtggt catcgaccag
     1921 ttcatcagtt cgggcgagcg caagtggctg cgcatgagcg gcctcgtcat gctgctgccg
     1981 cacggctacg agggccaggg gccggagcac agctcggccc ggctggagcg cttccttcag
     2041 tcctgcgccg aggacaacat gcaggtgctc aactgctcga ccccggccaa ctatttccac
     2101 gccctgcgcc ggcagatgca ccgcgagttc cgcaagccgc tgatcgtcat gacgcccaag
     2161 agcctgctgc ggcacaagaa ggcggtcagc aacctcgccg acctggccga gggctcgtcc
     2221 ttccaccggg tgatggtcga cggggccgag gccggctgcg acgtcggcgg gatcaccctc
     2281 aagcccgacg accagatcac ccgcgtgatc gcctgctcgg gcaaggtcta cttcgacctg
     2341 atcgacgccc gggccaaggc cgggcgcgac gacgtctaca tcgtgcgtct cgagcagttc
     2401 tatccctggc cgatgaagtc ggtgatgcag gtgatgggcc gcttcgccaa cgccgagctg
     2461 gtctggtgcc aggaggagcc gaagaacatg ggcggctgga ccttcgtcga tccgtggctg
     2521 gaactgacgc tcgaccgcat gagcatcaag gccaggcgcg cccgctacgt cggccggccg
     2581 gcctccgcct cgaccgccgc cggcctgatg agccggcacc tgaaagaact cgagaccttc
     2641 ctcgccgaag cgttcgcctg acgcccgcgc ccgacccgta cgagacacga aagacctgac
     2701 catggccgac atcatgactc ccgccctggg cgaatccgtc accgaggcga cggtcgcccg
     2761 ctggacgaaa aagccgggcg acgcggtcag gaaggacgag atcctcgtcg agctggagac
     2821 cgaca
//
LOCUS       DAORNX010000381         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27003_length_2812_cov_6.070366, whole genome shotgun sequence.
ACCESSION   DAORNX010000381 DAORNX010000000
VERSION     DAORNX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27003_length_2812_cov_6.070366"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PLO65_13990"
     CDS             <1..1584
                     /locus_tag="PLO65_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRD29405.1"
                     /translation="DQTAKRKLQEAVMARRLYRMLSRDEILELYLNRIFFGANTYGID
                     GASRAYFGKPAAQMSISEAALLASLPKAPSRLALTKNMAGALERQRLILGNMRREHWI
                     TEAQYEAALADTPRLAPGAAQEEGELGYVLDYATVEAVKIAGQNSPDLVVRLTIDSRL
                     QAAGTRILREIMRTDGAKAGATQAALLALGNDGAIRAMVGGTDFDETPFNRAVQARRQ
                     PGSTFKPFVYAAALEKGVLPTDQRVDGPVKFGDWAPENYGGGYSGPVTIETALAKSIN
                     TVAAKLGQEVGGAAMGELARRFGLSTIPPNPDLSVALGSYEVSLIQLVAAFQVFQQGG
                     QRRTPYIIDSITTVAGEPIYARQVNAGGVPVYDIAKASMMVRMMKGVIERGTGTRAAF
                     GRPAAGKTGTSQNWRDAWFIGFTPDITAGVWIGNDDDRPMNHVAGGTLPAAIWRRFMI
                     VAHEGLPVRDFDWLLPDPVPEEESDPRNPFYGELASDFARAADALEPAIAAPETPPAP
                     AEPARPEMPRLPSGEPIPY"
     gene            1710..2207
                     /locus_tag="PLO65_13995"
     CDS             1710..2207
                     /locus_tag="PLO65_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29406.1"
                     /translation="MVKKSGAAGLLAGLAEMLAAGLLEDLTVLAALPRPETDIALERR
                     VRLRGVIARAGLSIHKLQAQEDHPGAPQDQDEADMDEQPDDPDALERKYVELQDRLDR
                     YARRAGLRACPDADSAEGPGGREGQLADASERRPTAAEGPVAHLAAARRPGVGQDLGR
                     RRLAG"
     gene            2098..>2812
                     /locus_tag="PLO65_14000"
     CDS             2098..>2812
                     /locus_tag="PLO65_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HRD29407.1"
                     /translation="MRANGDQQPPRDPWRTWLLLGGRGSGKTWVGAGWLVEQAWRGGR
                     FALVGPSLHDVAEVMIEGPSGLRAQSPRSLGLRHEKSRRRVVWNTGAEAHIFSAEDPD
                     SLRGPQFHGAWADEFCAWRRPGEVLAMLRFGLRLGEDPRLLVTTTPRPIRALRMLRAE
                     AGTVDTVIPTRTNAHNLAPAFVEALNAIYGGTSLAAQELEGLIVEGEGALWTAAGLAA
                     CRGHRPPDFDQVVVAVDPPA"
BASE COUNT          434 a          989 c         1007 g          382 t
ORIGIN      
        1 gaccagacgg ccaagcgcaa gctgcaggag gcggtcatgg cccgccgcct gtaccggatg
       61 ctgagccgcg acgagatcct cgagctttac ctcaaccgaa tcttcttcgg ggccaacacc
      121 tacggcatcg acggagcctc gcgggcgtat ttcggcaagc ccgccgcgca gatgagcatt
      181 tccgaggcgg ccctgctggc cagcctgccc aaggccccct cgcggctggc cctgaccaag
      241 aacatggccg gcgccctgga gcggcagagg ctgatcctgg gcaacatgcg gcgcgagcac
      301 tggatcaccg aggcccagta tgaggcggcc ctggccgaca ccccgcggct ggccccgggc
      361 gccgcccagg aagagggcga gctgggctac gtgctcgact acgccacggt cgaggcggtc
      421 aagatcgccg gccagaactc gcccgacctg gtggtccggc tgaccatcga ctcccggctg
      481 caggcggccg gcacgcggat cctgcgcgag atcatgcgga ccgacggggc caaggccggg
      541 gcgacccagg cggcgctgct ggcgctgggc aacgacgggg cgatccgggc catggtcggg
      601 ggcaccgact tcgacgagac gccgttcaac cgggccgtcc aggcgcggcg ccagcccggc
      661 tcgacgttca agccgttcgt ctacgccgcc gctctggaaa agggcgtgct gcccaccgac
      721 cagcgggtcg acggcccggt caagttcggt gactgggcgc ccgagaacta tggcggcggc
      781 tattcgggtc cggtgaccat cgagacagcc ctggccaagt cgatcaacac cgtggccgcc
      841 aagctggggc aggaggtcgg cggcgcggcc atgggcgagc tggcgcggcg cttcggcctg
      901 tcgaccattc cacccaatcc cgacctgtcc gtggccctcg gctcgtacga ggtgtcgctg
      961 atccagctgg tggccgcctt ccaggtgttc cagcagggcg gccagcgtcg cacgccgtac
     1021 atcatcgaca gcatcacgac cgtcgccggc gagccgatct acgcccgcca ggtcaacgcc
     1081 ggcggcgtac cggtctatga catcgccaag gcctcgatga tggtgcggat gatgaagggc
     1141 gtcatcgaac gggggacggg cacgcgcgcc gccttcggcc gccccgccgc cggcaagacc
     1201 ggcaccagcc agaactggcg cgacgcctgg ttcatcggct tcacccccga catcaccgcc
     1261 ggggtctgga tcggcaacga cgacgaccgg ccgatgaacc atgtcgccgg cggcacgctt
     1321 ccggcggcca tctggcggcg gttcatgatc gtcgcccatg aagggctgcc ggtgcgcgat
     1381 ttcgactggc tcctgcccga tcccgtgccc gaggaggagt ccgatccgcg caacccgttc
     1441 tacggggagc tggcctccga cttcgcccgc gcggcggacg ctctcgagcc ggcgatcgcc
     1501 gcccccgaga cgccgcccgc accggccgag cctgccaggc cggaaatgcc gcggttgccg
     1561 tccggcgagc cgatccccta ctagggaaaa gcgggcgttc agcggccgcg cccccggact
     1621 atccggaaac aaaggccggt gaatcaacag cttgtcgctt tttcgcggca agcgcccgac
     1681 cgggcccggt gatcccgtag tcttggggca tggtcaagaa atcaggtgcg gcggggctgc
     1741 tcgcggggct ggcggagatg ctggcggcgg gcttgctgga ggacctgacg gtcctcgccg
     1801 cccttcccag acccgaaaca gatatcgccc tggagcggcg ggtgcggctg cgtggcgtca
     1861 tcgcccgcgc cggcctgtcg atccacaagc tgcaggccca ggaagatcat cccggcgcgc
     1921 cgcaggatca ggatgaggcc gacatggatg aacagcccga cgaccccgac gcccttgagc
     1981 ggaagtacgt ggaacttcag gacagactcg atcgatatgc tcgccgcgcc gggctcaggg
     2041 catgcccgga cgcggattcc gccgaaggac ctggaggtcg tgagggccaa ctggcggatg
     2101 cgagcgaacg gcgaccaaca gccgccgagg gacccgtggc gcacctggct gctgctcggc
     2161 ggccgggggt cgggcaagac ctgggtcggc gccggctggc tggttgagca ggcctggcgc
     2221 ggcgggcggt tcgccctcgt cggccccagc ctgcacgatg tggccgaggt gatgatcgag
     2281 ggcccgtccg gcctgcgggc gcagagtccc aggtccctgg gcctgcgcca cgagaaatcc
     2341 cgccgacggg tggtgtggaa caccggcgcc gaggcgcaca tcttttccgc cgaggatccc
     2401 gacagcctgc gcggcccaca gttccacggc gcctgggccg acgagttctg cgcctggcgg
     2461 cgaccgggcg aggtgctggc gatgctgcgg ttcggcctgc ggctggggga ggatccccga
     2521 ttgctggtga ccacgacacc ccggccgatc cgcgccctgc ggatgctgcg ggccgaggcc
     2581 ggcaccgttg atacggtgat cccgacccgg accaacgccc acaatctggc gccggccttc
     2641 gtcgaggccc tgaacgccat ctacggcggc accagcctgg ccgcgcagga gctggagggc
     2701 ctgatcgtcg agggcgaggg cgccctgtgg acggcggcgg gcctggcggc ctgtcgcggc
     2761 catcgcccgc cggacttcga ccaggtggtg gtggcggtcg acccgccggc ca
//
LOCUS       DAORNX010000382         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27183_length_2798_cov_8.693766, whole genome shotgun sequence.
ACCESSION   DAORNX010000382 DAORNX010000000
VERSION     DAORNX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27183_length_2798_cov_8.693766"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLO65_14005"
     CDS             <1..913
                     /locus_tag="PLO65_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD29408.1"
                     /translation="WVMFNPLPTFHCFGLTGGVLLPILTGMKAFQYPSPLHIKQIPAL
                     VKDSKASILLATDTFVNQYARASDSDELSGLQFIVCGAEKVRDETHNLIAERFGDVPV
                     LEGYGATEAAPVVAVNKPTDNRRGTVGGMLPGMETRIETVEGIAGGGRLFLRGPNVMA
                     GYLNDKGGVDPLPDGWHDTGDVVTIEGDDRWVTIKGRVKRFAKIGGEMVSLTAAEDLA
                     VAVWPEGRHAVIAMPDKKKGERLILFTDRPDASVAPLLAHAQTLGAPELAVPRKIVKI
                     PEVPVLGTGKTDYVAIQRLAETEARAA"
     gene            complement(981..1658)
                     /locus_tag="PLO65_14010"
     CDS             complement(981..1658)
                     /locus_tag="PLO65_14010"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRD29409.1"
                     /translation="MSRDRMAVLAAMIDQGVIPVFHHPDPAICIRVIAACAAGGARCI
                     EFTNRGPFAAHTFLEIARHFADADPSVIMGVGSIVDAPTAGLFIANGADFVVGPALNP
                     EVARLCNRRMVAYSPGCATASEIGLAHELGCEIIKLFPGETVGGPAFVRNLLGPMPWT
                     KIMPTGGVEPEEGSLRDWFGAGIVAAGMGGRLISKDLVEAGDWAGLEARVRRTVELIA
                     RIRRDLA"
     gene            1807..2268
                     /locus_tag="PLO65_14015"
     CDS             1807..2268
                     /locus_tag="PLO65_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD29410.1"
                     /translation="MKDLRARFIAELDRRRVLSLATLRPDGWPQATIVGYVNDGPAIY
                     FMISRESQKFANLTADPRVSICVGEDVADPMAIGGASMAATVAEVTDSAERARAMALM
                     FKRYPEYEGMMKDVDTTQMGLMRATPFIASVLTYEKGFGHTDELRLAARPS"
     gene            2377..>2798
                     /locus_tag="PLO65_14020"
     CDS             2377..>2798
                     /locus_tag="PLO65_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HRD29411.1"
                     /translation="MAQAKLATYRAKRDFSITAEPSGEVAVAASRRRRFVIQKHAATR
                     LHYDLRLEHEGVFLSWAVTRGPSRDPADKRLAVEVEPHPLDYGDFEGTIPKGEYGGGT
                     VMLWDRGYWAPEPGFDVAKGLKKGELKVVFAGERMQGG"
BASE COUNT          452 a          970 c          966 g          410 t
ORIGIN      
        1 ctgggtgatg ttcaacccgc tgccgacctt ccactgcttc ggcctgaccg gcggagtgct
       61 gctgccgatc ctgaccggca tgaaggcctt ccagtacccc tcgccgctgc acatcaagca
      121 gatcccggcg ctggtgaagg acagcaaggc ctcgatcctg ctggccaccg acaccttcgt
      181 gaaccagtac gcccgcgcgt cggattccga cgaactgtca ggcctgcagt tcatcgtctg
      241 tggcgccgag aaggtgcgcg acgaaacgca caacctgatc gccgagcggt tcggcgacgt
      301 gcctgtgctc gagggctacg gggcgaccga ggccgcgccc gtggtggcgg tcaacaagcc
      361 gaccgacaat cgtcgcggca cggtcggcgg catgctgccg ggcatggaga cgcgcatcga
      421 gacggtcgag ggtatcgccg gcggcgggcg gctgttcctg cgcggcccga acgtcatggc
      481 cggctacctg aacgacaagg gcggggtcga tcccctgccc gacggctggc acgacaccgg
      541 cgacgtggtg accatcgaag gcgatgaccg ctgggtgacc atcaaggggc gggtcaagcg
      601 cttcgccaag atcggcggcg agatggtctc cctgaccgcg gccgaggacc tcgccgtggc
      661 cgtctggccg gagggccgcc atgccgtgat cgccatgccc gacaagaaga agggcgaacg
      721 gctgatcctg ttcaccgacc ggccggacgc gtcggtggcg ccgctgctgg cgcacgccca
      781 gacgctcggc gcgcccgagc tcgcggtgcc ccgaaagatc gtcaagattc cggaagtccc
      841 cgtgctggga accggaaaga ccgactacgt cgccatccag cgcctggcgg aaacggaggc
      901 gcgggcggcg tagggtttcc tgacggggaa acgacgccca agcgatccct ggaccggccc
      961 ggcggcgcgg gagatccggt tcaggccagg tcgcgccgga tccgcgcgat cagctcgacg
     1021 gtccggcgca cgcgcgcctc gagtcccgcc cagtcgccgg cctcgacgag gtccttgctg
     1081 atcagccgcc cgcccatgcc ggccgcgacg atgcccgccc cgaaccagtc gcgcagggac
     1141 ccctcctcgg gctcgacgcc gccggtcggc atgatcttgg tccagggcat cgggcccagg
     1201 aggttgcgca cgaaggcggg gccgccgacc gtctcgcccg gaaacagctt gatgatctcg
     1261 cagcccagtt catgggccag gccgatctcg ctggcggtgg cgcaacccgg gctgtaggcg
     1321 accatccgtc ggttgcacag ccgggcgacc tcgggattca gcgccgggcc gacgacgaaa
     1381 tccgcgccgt tggcgatgaa cagcccggct gtcggggcgt cgacgatgct gcccacgccc
     1441 atgatcaccg acggatcggc gtcggcgaaa tgccgggcga tctcgagaaa ggtgtgcgcc
     1501 gcgaacgggc cgcggttggt gaactcgatg cagcgcgcgc cgccggcggc gcaggcggcg
     1561 atcacccgga tgcagatcgc cggatcgggg tggtgaaaga cggggatcac cccctggtcg
     1621 atcatggcgg ccagaacggc catgcggtcc ctggacatgg ttcggcttct cctcgcgccc
     1681 cagccttacg cctgcctgcg gaaaaaagaa ggccgccgcg cgacggcgcc gtggtccgtc
     1741 ccatgatctc aatcaaggct cggctcgggc cggtcgccat gatgccggcg actagggagc
     1801 aggctgatga aagatctcag ggcccggttc atcgccgaac tcgacaggcg gcgcgtcctg
     1861 tcgctcgcca ccctccgccc ggacggctgg cctcaggcga ccatcgtcgg ctacgtcaac
     1921 gacggcccgg cgatctactt catgatctcg cgcgagagcc agaagttcgc caatctgacg
     1981 gcggacccgc gtgtgtcgat ctgcgtcggt gaggacgtcg ccgaccccat ggctatcggc
     2041 ggcgcctcga tggccgcgac ggtggccgag gtcaccgact ccgccgaacg ggcgcgcgcc
     2101 atggccctga tgttcaagcg ctatcccgaa tacgagggga tgatgaagga tgtcgacacc
     2161 acccagatgg gtctgatgcg cgccaccccg ttcatcgcct ccgttctgac ctacgaaaag
     2221 ggcttcggtc acaccgacga actgcggctt gcggcccggc cttcgtagtc gtcgccatcg
     2281 ccccccctgg ccttggcatg ggtcaatttg ccggcgcgtc tggaaccctg gactgcccgc
     2341 gcgtcttcca tcggcgaaca gccggaggtt tccaccgtgg cccaggccaa gctcgcgacc
     2401 taccgcgcca agcgcgactt ctcgatcacc gccgagccga gcggcgaagt cgccgtcgcc
     2461 gcctcgcgac gccgccggtt cgtgatccag aagcacgcgg ccacgcgcct gcactacgac
     2521 ctgcggcttg agcacgaggg cgtcttcctg tcgtgggccg tgacgcgggg cccctcgcgc
     2581 gatccggctg acaagcggct ggccgtcgag gtcgagccgc acccgctcga ctacggcgat
     2641 ttcgagggga ccatccccaa gggcgagtac ggcggcggca cggtgatgct gtgggatcgc
     2701 ggctactggg cgcccgagcc cggcttcgac gtcgcgaagg gcctgaagaa gggtgagctg
     2761 aaggtcgtct tcgccggcga gcgcatgcag ggcggcta
//
LOCUS       DAORNX010000383         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27232_length_2794_cov_6.544359, whole genome shotgun sequence.
ACCESSION   DAORNX010000383 DAORNX010000000
VERSION     DAORNX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27232_length_2794_cov_6.544359"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="narH"
                     /locus_tag="PLO65_14025"
     CDS             <1..488
                     /gene="narH"
                     /locus_tag="PLO65_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261108.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRD29412.1"
                     /translation="RTLPMVWYVPPLSPIQSAAASGALEHDGQMPDVSSLRIPVRYLA
                     NLLTAGNEPPVVLALERMLAMRGFMRARTVEGVTDHALADRVGLTAGQIEEMYRYMAI
                     ANYEDRFVIPTTHRETAEDAYELRGDCGFSFGNGCSGGRTEVGLFGSDPRSRAKPLME
                     V"
     gene            490..1194
                     /gene="narJ"
                     /locus_tag="PLO65_14030"
     CDS             490..1194
                     /gene="narJ"
                     /locus_tag="PLO65_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRD29413.1"
                     /translation="MALTYKALAVLLAYPGADTQQLAKAAIQAIEDEGMVPVQVRRSL
                     ARLADELADGDLFELQERYVWQFDRTRSLSLNLYEHVHGESRDRGQAMVSLLELYRRK
                     GLELSANELPDHLPVFLEFLSLLPEPEAASLLGEAAHVLAAMGERLHKRQSPYRAVFG
                     ALTALAGAEADAAALASLMQEPDDDPGDFETLDRLWAETAVTFGPSDVGCPKAEGLVR
                     AMNDAPAPVRRRAGAA"
     gene            1205..1933
                     /gene="narI"
                     /locus_tag="PLO65_14035"
     CDS             1205..1933
                     /gene="narI"
                     /locus_tag="PLO65_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRD29414.1"
                     /translation="MDLNHILFGVYPYIALSVLAIGSVVRFDREQYTWRTGSSQLLRR
                     RQLVLGSVLFHVGVLAIFAGHFVGLLTPIAVFDALHVPHSAKQLLAIVAGGGAGALCL
                     VGGAMLLHRRLFDPRIRATSTFGDTAILIMLMVQLLLGLSTIPQSLKHMDGHEMVKFM
                     GWAQGIFTLRPGAADLLTDVHWVFKIHIVLGLTILLVFPFTRLVHMLSAPVWYLNRRG
                     WQLVRTRRPVPPRRIDTRRQGGVS"
     gene            1930..>2794
                     /locus_tag="PLO65_14040"
     CDS             1930..>2794
                     /locus_tag="PLO65_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD29415.1"
                     /translation="MSALRAIVTDGVQIPESLIAQEAQNHPGGSPDEVRAAAAHALAV
                     RALLLDRAAELGLVAEPELDERGREETPEEALVRAVLAAEVEPETPTEDECRRVYDSR
                     PDRFRSPTLYEASHILFAIDGGGDSASETARMSAQTILSRLRDEPGRFSTLAAAYSAC
                     PSSGVGGSLGQLGPGDLAPEVETAFGRLAAGEISAEPVRSRFGWHILRLDRRIDGRTL
                     PFEQVADRIRLHLESRAWVAAAVQYVSDLAARARSRGVGLVISADGEVSAGATLGDLI
                     AATPDGAPEDLL"
BASE COUNT          357 a         1031 c          962 g          444 t
ORIGIN      
        1 accggacgct gccgatggtc tggtacgtgc cgccgctgtc gccgatccag tcggcggccg
       61 cctccggcgc gctggagcac gatggccaga tgccggacgt ctccagcctg cgcatcccgg
      121 tccgctatct ggccaacctg ctgaccgccg gcaacgagcc gcctgtcgtg ctggcgctgg
      181 agcggatgct ggccatgcgc ggcttcatgc gcgcccgcac cgtcgagggg gtcaccgacc
      241 acgccctggc cgatcgggtc ggcctgacgg ccggccagat cgaggagatg taccgctaca
      301 tggcgatcgc caactacgag gaccggttcg tcatccccac cacccatcgc gagaccgccg
      361 aggacgccta cgagctgcgc ggcgactgcg gcttctcgtt cggcaacggc tgctccggcg
      421 gccgcaccga ggtcggcctg ttcggctccg atccgcgctc gcgcgccaag cctctgatgg
      481 aggtctgaga tggcgctcac ctacaaggcg ctggccgtgc tgctggccta tcccggcgcc
      541 gacacgcagc agctcgccaa ggccgcgatc caggcgatcg aggacgaggg catggttccg
      601 gtccaggttc gtcgttcgct ggcgcgcctg gctgacgagc tggccgacgg cgacctgttc
      661 gagctgcagg aacgctatgt ctggcagttc gaccgcaccc gctcgctgtc gctgaacctc
      721 tacgagcacg tgcacggcga gagccgcgac cgcggccagg ccatggtcag cctgctggag
      781 ctctaccgcc gcaagggact ggagctgtcg gccaacgagc tgcccgatca cctgccggtg
      841 ttcctcgagt tcctgtcgct gctgcccgag ccggaagccg cgtccctgct cggcgaggcg
      901 gcgcatgtcc tggccgccat gggcgagcgc ctgcacaagc ggcagagccc gtatcgcgcg
      961 gtgttcggag ccctgaccgc cctggccggc gccgaggcgg acgccgcggc gctcgcttcg
     1021 ctgatgcagg agccggacga cgatccgggc gacttcgaga cgctcgaccg gctgtgggcc
     1081 gagacggccg tgaccttcgg cccgtccgac gtcggctgtc ccaaggccga aggcctcgtc
     1141 cgcgccatga acgacgcccc cgcgcccgtc cgtcgccgcg ccggcgcggc ctgaggagct
     1201 tacgatggac ctcaatcaca tccttttcgg cgtctatccc tacatcgccc tgtcggtgct
     1261 ggcgattggc tcggtcgtgc gcttcgaccg cgagcagtac acctggcgca ccgggtcctc
     1321 gcagctgctg cggcgacgcc agctggtcct gggctcggtg ctgttccatg tcggcgtgct
     1381 ggccatcttc gcgggtcact tcgtcggtct gctgaccccc atcgcggtgt tcgacgcgct
     1441 gcatgtgccg cactcggcca agcagctgct ggccatcgtc gcgggcggcg gggcaggagc
     1501 cctgtgcctc gtgggcggcg ccatgctgct gcatcgccgg ctgttcgacc cgcgcatccg
     1561 ggccacgtcc accttcggcg acaccgccat cctgatcatg ctgatggtcc agctgctgtt
     1621 gggcctatcg accatcccgc agtcgctcaa gcacatggac gggcacgaga tggtcaagtt
     1681 catgggctgg gcccagggca tcttcacgct gcgccccggc gcggccgacc tgctgaccga
     1741 cgtgcactgg gtgttcaaga tccacatcgt gctgggtctg acgatcctgc tcgtgttccc
     1801 cttcacccgc ctggtccaca tgctgtcggc cccggtctgg tacctgaacc gtcgcggctg
     1861 gcagctggtg cgcacgcgcc ggcccgtgcc gcctcgccgc atcgacaccc gccgtcaggg
     1921 aggcgtgtca tgagcgcgct gcgcgccatc gtcaccgacg gggtgcagat ccccgagagc
     1981 ctgatcgccc aggaggccca gaaccatccg ggcggatcgc cggacgaggt gcgcgccgcc
     2041 gccgcccacg ccctggccgt gcgggctctg ctgctcgacc gcgcggccga actgggtctg
     2101 gtcgccgagc cggagctgga cgagcgcggc cgcgaggaga ccccggagga ggccctggtg
     2161 cgggccgtgc tggccgcgga ggtcgagccg gagacgccga ccgaggacga gtgccggcgg
     2221 gtctacgact cgcggcccga tcgcttccgc tcgccgacgc tgtacgaggc gtcgcacatc
     2281 ctgttcgcga tcgatggcgg cggcgactcg gccagcgaga ccgcgcggat gtctgcgcag
     2341 accatcctgt cccgccttcg cgacgaaccc ggccggttct ccacgctggc ggcggcctat
     2401 tcggcctgcc cctcaagcgg cgtcggtgga tcgctgggcc agctgggtcc cggcgatctc
     2461 gcgccggagg tcgagacagc cttcggtcgc ctggccgcgg gcgagatttc ggccgagccc
     2521 gtccgctcgc gcttcggctg gcacatcctg cgcctggatc gccgcatcga cggccgcacc
     2581 ctgccgttcg agcaggtcgc cgaccgcatc cgcctgcatc tcgagagccg ggcatgggtc
     2641 gcggcggccg tccagtacgt ctccgacctg gcggcccgcg cccggtcgcg cggcgtgggc
     2701 ctggtcatct ccgccgatgg cgaggtgtca gccggcgcca ccctgggcga cctgatcgcc
     2761 gccacccccg acggcgcacc ggaggacttg ctgg
//
LOCUS       DAORNX010000384         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27250_length_2793_cov_4.707451, whole genome shotgun sequence.
ACCESSION   DAORNX010000384 DAORNX010000000
VERSION     DAORNX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27250_length_2793_cov_4.707451"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLO65_14045"
     CDS             complement(<1..183)
                     /locus_tag="PLO65_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRD29416.1"
                     /translation="MPEPTLTLADDFAPPSRQDWLALVEKTLKGETFEDALVRRTADG
                     IAIAPLYTDAPATVRDL"
     gene            326..1333
                     /locus_tag="PLO65_14050"
     CDS             326..1333
                     /locus_tag="PLO65_14050"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRD29417.1"
                     /translation="MALPPILRDRLRLPAIASPLFIISNPDLVIAQCKAGIVGSFPSL
                     NARPISLLDEWLDRITSELAAWDKANPDLPSAPFAVNQIVHKTNNRLDEDMALCAKYR
                     VPVVITSLGAREDVNEAVHGWGGITLHDVITDRFAHKAIEKGADGLIPVAAGAGGHAG
                     GLSPFAMIQEIRQWFDGPVALSGSIANGAAILAAQAMGADLAYIGSAFIATKEANAVE
                     GYKQMIVDCSGEDIVYSNLFTGIHGNYLRPSIVAAGMDPDNLPQSDPSAMNFGSGGNT
                     EAKAWKDIWGCGQGIGAVKSVLGAGELVARLSAEYEAAKADLAAKTALTSGVRLAFAA
                     E"
     gene            1465..2202
                     /locus_tag="PLO65_14055"
     CDS             1465..2202
                     /locus_tag="PLO65_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HRD29418.1"
                     /translation="MSDDATPRLGLPYVAAGQAQKHVTVNEALARLDGMVQASVVSRS
                     VAVQPATPGDGALYLLPDAASGDVWSGEAAGALMRFEAGSWTRLQPGPGHLLWVADEG
                     LVLVFDGVWRPLAETLAFDALVAAASPGGARTRFGIREEDVALAGPVTATTLAIPARS
                     IVLAVATRTVAAVTGATAYDCGVAGETGKFGGSLGVAAGSGNIGVIGPTAFYADTPVL
                     LTAIGGDFTGGVVRVAAHLVEFDAPGA"
     gene            2213..2737
                     /locus_tag="PLO65_14060"
     CDS             2213..2737
                     /locus_tag="PLO65_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29419.1"
                     /translation="MDIITAAGGGLAGGALGAAFGALGSILNRGLGIFETREKRKDRQ
                     LEMAHEVERWDHDLKLAAEAREGRIEAAKQALARADAEGSWRGLDASLQAEAGIGQSW
                     PWVTAVRALTRPVLTLLLWLLFAVLFLSAVSGALDSATASGVVRTAVETIAFSASTAL
                     AWWFGDRTPRRGAG"
BASE COUNT          397 a          973 c          991 g          432 t
ORIGIN      
        1 caggtcgcgg accgtcgctg gcgcgtccgt atagagggga gcgatggcga tgccgtcggc
       61 cgtccgccgg accagggcgt cctcgaaggt ctcgcccttg agagtcttct cgaccagggc
      121 cagccagtcc tggcgggatg gcggcgcgaa gtcatcggcc agcgtcaggg tcggttcagg
      181 cacgccttgc gtctcccaga gtgcgatgca gcatcagcgg cttcgggtca tgcgcccctg
      241 ccgcaaggtg cgtcaagcga gggcttgtcg aactcgaacg cttgttctat ttacaacgta
      301 tattcaaaga cggagacatt ctgccatggc cctgccgccg atcctgcgtg accgcctgcg
      361 actgccggcc atcgcctcgc cgctgttcat catttccaac ccggacctgg tgatcgccca
      421 gtgcaaggcg ggcatcgtcg gctcgttccc gtcgctgaac gcccggccga tcagcctgct
      481 ggacgagtgg ctcgaccgga tcaccagcga gctggcggcc tgggacaagg ccaatccgga
      541 cctgccgtcc gcgccgttcg cggtcaacca gatcgttcac aagaccaaca accggctcga
      601 cgaggacatg gccctctgcg ccaagtacag ggtcccggtc gtcatcacat cgctgggcgc
      661 ccgcgaagat gtcaacgagg cggtgcacgg ctggggcggc atcaccctgc atgacgtgat
      721 caccgacagg ttcgcccaca aggccatcga gaaaggcgcc gacggcctga ttccggtcgc
      781 ggccggcgcc ggcggccatg ccggcggcct gtcgccgttc gccatgattc aggaaatccg
      841 ccagtggttc gacggaccgg tggcgctgtc gggttcgatc gccaatggcg cggccatcct
      901 cgccgcccag gccatgggcg cggacctggc ctacatcggc tcggccttca tcgccacgaa
      961 ggaggccaac gccgtcgagg gctacaagca gatgatcgtc gactgctccg gcgaagacat
     1021 cgtctattcg aacctgttca ccggcatcca cggcaactac ctgcggccgt cgatcgtcgc
     1081 cgccggcatg gacccggaca acctgcccca gagcgacccc tcggcgatga acttcggctc
     1141 gggcggcaac accgaggcca aggcctggaa ggatatctgg ggctgtggcc agggcatcgg
     1201 cgcggtcaag tcggtgcttg gcgccggcga gctggtcgcc cggctgtcgg cggaatacga
     1261 ggcggccaag gccgatctcg cggccaagac ggccctgacc agcggcgtcc gcctggcgtt
     1321 cgcggccgag taaccgccgg ccggccccgc cggatcagtg acttgcgccc ggcgatccat
     1381 cgtgatcgcc gggcgttttc ggtttcggcg cggtcgccgt tggcgcggac ccatccttgt
     1441 cgacgccaac gacagggagc ccgcatgtcc gacgacgcca caccccgcct cggcctgccc
     1501 tatgtggcgg ccggccaggc ccagaaacac gtcaccgtca acgaggccct cgcccggctc
     1561 gatggcatgg tccaggcgtc ggtggtcagc cgctcggtcg cagtccagcc agcgacgccc
     1621 ggtgacgggg ccctctatct gctgcccgac gcggcctccg gcgacgtctg gagcggcgag
     1681 gcggccggcg ccctgatgcg tttcgaggcc ggaagctgga cccgcctgca gccgggcccg
     1741 ggccacctcc tctgggtggc cgacgagggc ctcgttctgg tgttcgacgg cgtctggcgg
     1801 cctctggcgg agacgctcgc cttcgacgcc ctggtcgcgg cggcctcgcc gggcggcgcc
     1861 cgcacccgct tcggtatccg cgaggaggat gtggcgctgg ccggtccggt gaccgcaacg
     1921 accctggcga tcccggcccg ctccatcgtg ctggcggtgg ccacgcgcac agtggcggcc
     1981 gtcaccggcg ccacggccta cgactgcggc gtggccggcg agaccggcaa gttcggcggc
     2041 tcgctcggcg tggccgccgg ctcaggcaat atcggggtga tcgggccgac ggccttctac
     2101 gccgacacgc cggtgctgct gacggccatc ggcggcgatt tcacgggcgg agtcgtgcgg
     2161 gtcgccgccc atctcgtcga attcgacgcg ccaggcgcgt gaggagctgc tcatggacat
     2221 catcaccgca gccggtggcg gactcgccgg aggagccctg ggcgcggcct tcggggcgct
     2281 gggctcaatt ctcaaccggg ggctgggcat tttcgagacg cgcgaaaagc gcaaggatcg
     2341 ccagctggaa atggcccacg aggtcgagcg ctgggatcac gatctcaagc tcgccgccga
     2401 ggcccgggag ggccggatcg aggcggcgaa gcaggcgctg gcgagggccg acgcggaggg
     2461 gtcctggcgc gggctggatg cttcgctgca ggccgaggcc ggcatcggcc agagctggcc
     2521 gtgggtgacg gcggtccgcg ccctgacccg gccggtcctg accctgctgc tgtggctgct
     2581 gttcgcggtg ctgttcctct cggcggtcag cggggcgctg gattccgcca cggcgtcggg
     2641 cgtggtccgc acggccgtcg agacgatcgc cttctcggct tccacggccc tggcctggtg
     2701 gttcggcgac cggacgccgc gacgcggcgc cggctgagca ggccttggcg ccgccgcgcg
     2761 gcggtctaga tccaggctgt ctgagaggga ccc
//
LOCUS       DAORNX010000385         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27390_length_2783_cov_7.152126, whole genome shotgun sequence.
ACCESSION   DAORNX010000385 DAORNX010000000
VERSION     DAORNX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27390_length_2783_cov_7.152126"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..918)
                     /gene="yghU"
                     /locus_tag="PLO65_14065"
     CDS             complement(43..918)
                     /gene="yghU"
                     /locus_tag="PLO65_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HRD29420.1"
                     /translation="MSDTPSYTPPRVWTWNKDNGGRFANINRPIAGPTHDKDLPVGKH
                     PLQLYSLATPNGVKVTVMLEELLALGHAGAEYDAWLIVISNGDQFGSGFVGINPNSKI
                     PALVDRSGPAPIRVFESGAILLYLAEKFGAFLPTDPATRAECLSWLMWQMGSAPYLGG
                     GFGHFYAYAPFKIEYAIDRFAMEVKRQMDVLDRRLAESEYLAGPDYTIADMAVWPWYG
                     AMAKGLLYEGGEFLQVQEYKNVQRWTDQIAERPAVQRGRMVNRGWGDPASQLPERHDA
                     SDFALRTADKLAAGG"
     gene            1128..1628
                     /locus_tag="PLO65_14070"
     CDS             1128..1628
                     /locus_tag="PLO65_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HRD29421.1"
                     /translation="MKRLILALTAAATVAGPMAFAATDALAQDHGRYDRRDDRWDRRD
                     DRRHDRWDRRDHRRDDRRDRRWDSRTRWDRGRHNGYYYNNRWHYGQPPAAYYGRPGYY
                     PGYNAWRRGAYLPPSYRGRGYVVYDYGRYRLRPPPRGYYWYRDGDDFLLAAVATGLIL
                     DVIVNH"
     gene            complement(1707..>2783)
                     /gene="gatB"
                     /locus_tag="PLO65_14075"
     CDS             complement(1707..>2783)
                     /gene="gatB"
                     /locus_tag="PLO65_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517891.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRD29422.1"
                     /translation="AGKSIHDLSPTETYVDLNRSGTALMEIVSKPDMRSPEDAAAYVK
                     KLRAILVTLGTCDGDMEKGNLRADVNVSVCRPGDYEKFRETGDFGHLGTRCEIKNVNS
                     YRFIQQAINYEARRQIEILEDGGRIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPD
                     LLPLELDPAWIKAIEASLPELPDAKKARLMAGYGLSAYDATVLTSDGDLAAYFEEAAK
                     GRDAKLVANWTLNDLLGRLGKDGLEIKDSPIPAADIAALVALIEDGTISGKIARTVFE
                     HMWAGEGAPSAIVEKHGLVQITDTGAIEAAVDALIAANPDKAAAVKDKPQAIGWFVGQ
                     VMKETGGKANPAAVNEILKAKLGL"
BASE COUNT          414 a          953 c          927 g          489 t
ORIGIN      
        1 cgtcggcgtt cgccgaccgc tgaggaaccg gccgccgtcc gatcagccgc cggcagccag
       61 cttgtcggcg gtcctgagcg cgaagtcgct ggcgtcgtgg cgctcgggca gctggctggc
      121 ggggtcgccc cagccgcgat tgaccatccg gccgcgctgc accgccgggc gctcggcgat
      181 ctggtccgtc cagcgctgga cgttcttgta ctcctgcacc tgcaggaact cgccgccctc
      241 gtagagcagg cccttggcca tcgcgccgta ccagggccag acggccatgt cggcgatcgt
      301 gtagtcgggg ccagcgaggt attcactttc cgccaggcgg cggtcgagca cgtccatctg
      361 gcgcttcacc tccatcgcga accggtcgat ggcgtattcg atcttgaacg gggcataggc
      421 gtagaagtgc ccgaagccgc cgccgaggta gggggcgctg cccatctgcc acatcagcca
      481 ggacaggcat tcggcgcggg tcgccggatc ggtcggcagg aaggcgccga acttctccgc
      541 caggtacagc aggatggctc ccgattcgaa gacccggatc ggcgcgggac cgctgcggtc
      601 gaccagggcc ggaatcttgg aattgggatt gatcccgacg aagccgctgc cgaactggtc
      661 gccgttgctg atcacgatca gccaggcgtc gtattccgcg ccggcatggc caagggccag
      721 cagttcctcc agcatcaccg tgaccttgac cccgtttggc gtggccaggg agtagagctg
      781 cagcgggtgc ttgccgaccg gaaggtcctt gtcatgggtc ggcccggcga tcgggcggtt
      841 gatgttggcg aaccggccgc cgttgtcctt gttccaggtc cagaccctgg ggggcgtata
      901 gctcggcgta tcggacatca ctgcggctcc tgacatggga atggggcggc ccttggacga
      961 catttggccg acaatcccgg ctttggctag acgaccgggc cgcggcgcgg gaacgccgaa
     1021 cctgaccaac ccgttcaggt cgggttcagg ccgaacgatt caccatcagg gcctgtcgag
     1081 ggaaagccgc gaccgagatg tcgctccgaa caagaggagg ccttcacatg aagcgtctga
     1141 tcctggccct gaccgccgcc gcgaccgtgg ccggtcccat ggccttcgcc gcgaccgatg
     1201 cgctggcgca ggaccacggc cgatacgacc gccgcgacga tcgctgggac cgccgcgacg
     1261 accgccgcca tgaccggtgg gaccgccgcg accaccgccg cgacgaccgc cgcgatcgcc
     1321 ggtgggacag ccgcacccgc tgggaccgcg gccggcacaa cggctactac tacaacaacc
     1381 gctggcacta cggtcagccg ccggccgcct attacggccg ccccggctac tatccgggct
     1441 acaacgcctg gcgtcgcggc gcctatctgc cgccgtccta ccggggccgt ggatatgtgg
     1501 tctatgacta tggccgctac cgcctgcgcc cgccgccccg cggctactac tggtaccgcg
     1561 atggtgacga tttcctgctg gccgctgtcg ccaccggcct gatcctggac gtgatcgtca
     1621 accattaggg tcatcgggga catgcacttc agtgcgtgtc ccctctggcc ggaacgggac
     1681 acgccctgaa gtgcatgtcc ctgcgcctac agccccagtt tcgccttgag gatctcgttg
     1741 acggcggccg gattggcctt gccgccggtc tccttcatca cctggccgac gaaccagccg
     1801 atcgcctgcg gcttgtcctt cacggcggcg gccttgtcgg ggttggccgc gatcagggcg
     1861 tcgacggccg cctcgatggc cccggtgtcg gtgatctgga ccaggccatg tttctcgacg
     1921 atggccgagg gcgcgccctc ccccgcccac atgtgctcga acaccgtgcg ggcgatcttg
     1981 cccgagatgg tgccgtcctc gatcagcgcc accagggcgg cgatatcggc ggccgggatc
     2041 ggcgagtcct tgatctcgag gccgtccttg ccgagccggc cgagcagatc gttgagcgtc
     2101 cagttggcga ccagtttggc gtcgcgcccc ttcgccgctt cctcgaaata ggcggccagg
     2161 tcgccgtctg acgtcagcac cgtggcgtcg taggccgaca ggccgtagcc ggccatcagc
     2221 cgcgccttct tcgcatccgg caattccggc agcgaggcct cgatggcctt gatccaggcc
     2281 ggatcgagct ccagcggcag caggtccggg tcggggaagt agcggtagtc gtgcgcctcc
     2341 tccttggacc gcatcgagcg ggtctcgccc ttgcccgggt cgaacaggcg ggtctcctgg
     2401 tcgatcctgc cgccgtcctc gagaatctcg atctgccggc gggcctcgta gttgatggcc
     2461 tgctggatga agcggtagct gttgacgttc ttgatctcgc agcgggttcc caggtggccg
     2521 aagtcgccgg tctcgcggaa cttctcgtag tcgcccggac ggcagaccga gacgttgacg
     2581 tcggcgcgaa ggttgccctt ctccatgtcg ccgtcacagg tgcccaacgt gaccaggatg
     2641 gcccgcagct tcttgacgta agccgccgca tcttcaggcg aacgcatgtc cggcttgctg
     2701 acgatctcca tcagggccgt ccccgaacgg ttgaggtcga cataggtctc ggtcggcgac
     2761 aggtcgtgga tcgacttgcc ggc
//
LOCUS       DAORNX010000386         2774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27515_length_2774_cov_6.559029, whole genome shotgun sequence.
ACCESSION   DAORNX010000386 DAORNX010000000
VERSION     DAORNX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27515_length_2774_cov_6.559029"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLO65_14080"
     CDS             complement(<1..476)
                     /locus_tag="PLO65_14080"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRD29423.1"
                     /translation="MTDVAIRSRSELRWILLLGLLVIAIVLAAGLGGTAFVARAVDRA
                     VATEERNLVVRTLGRRQAKLIEDITSATVWDDAYVRTVQAFDAQWADENYGVYYATYM
                     HHDRTLVFGPDGAVIYASDGGEAVEPSGQAAFARDLGPLLTAVRRAEAQKHANGAAP"
     gene            612..1310
                     /locus_tag="PLO65_14085"
     CDS             612..1310
                     /locus_tag="PLO65_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRD29424.1"
                     /translation="MIRFMSMVAVCTSLTFVGVAQAQEADEDGWSLSGEVGLVSDYQY
                     RGYSLSGGDPVVQGGLTLDAPGGFYVGVWGSEIEEYGVGADGDGAKIEVDISVGRAFS
                     AGGWDWDVALLHYGYPDGSAVDYFEIPVSAARTTGDFTTTLGVEYAPRQDNLGDEDNL
                     YLYIGGDYAPESWPVSLSARLGHEEGAFADGKLDWQLGVAKELGPVSLTLQYTDSDGP
                     GAESAAVAGISVGF"
     gene            complement(1482..1691)
                     /locus_tag="PLO65_14090"
     CDS             complement(1482..1691)
                     /locus_tag="PLO65_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD29425.1"
                     /translation="MATGTVKWFNATKGYGFIQPDDGGADVFVHISAVERAGMRSLNE
                     GQKLSYELERDNRSGKTSAGQLQAS"
     gene            complement(1858..2223)
                     /locus_tag="PLO65_14095"
     CDS             complement(1858..2223)
                     /locus_tag="PLO65_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29426.1"
                     /translation="MIEEGFVRMYAHDFAALAARAEGGSDVEALVRKRIDEARSHAAL
                     MDARKGEGHLPAVADRVAHEAEREDVRFVRAGQDVPGALARRKEFLLQIVDLLRSPPV
                     AAGRVSIAVRGQSDSRRLA"
     gene            2449..>2774
                     /locus_tag="PLO65_14100"
     CDS             2449..>2774
                     /locus_tag="PLO65_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD29427.1"
                     /translation="MSLTHRLAAPDDIPALTTLMDAAIGALQAPFLTPGQIAASRRLM
                     GLDSQLIADRTYFVVEADGVIAGCGGWSRRATLYGGDHSPGRDAHGLNPAEDAARVRA
                     MYTRPG"
BASE COUNT          445 a          917 c          957 g          455 t
ORIGIN      
        1 ggagcggcgc cgttcgcgtg cttctgcgcc tcggcccgcc gcacggcggt cagcagcggc
       61 cccaggtccc gggcgaacgc ggcctggccg gacggctcga ccgcctcgcc gccgtcggat
      121 gcgtagatca ccgccccgtc gggcccgaac accagggtcc ggtcatgatg catgtaggtg
      181 gcgtagtaga cgccatagtt ctcgtcggcc cattgggcgt cgaaggcctg cacggtcctg
      241 acgtaggcgt cgtcccagac cgtggccgag gtgatgtcct cgatgagctt ggcttggcgg
      301 cggccgaggg tgcggacaac gaggtttcgt tcctcggtcg ccaccgcccg gtcgaccgcc
      361 cgcgccacga aggcggttcc gccgagtccg gcggcgagca cgatggcgat caccagcaga
      421 cccagcagca ggatccacct cagctcgctc cggctgcgga tggcgacatc ggtcatgcac
      481 tcattctggg caggaaggcc taacatgcct tgaacccgaa atgagccgac aagtcgcagg
      541 actactggcg gctttcggct ctcgttaatt cagtttaggg actctgccgc cgggacaagg
      601 gggaagccag aatgatcagg ttcatgtcga tggtcgctgt ctgtacgtcg ctgaccttcg
      661 tgggcgtcgc acaggcgcag gaggcggacg aagacggctg gagtctttcg ggtgaggtgg
      721 gactggtgtc tgactaccag taccgtggct actcgctgtc gggcggcgat ccggtggtcc
      781 agggcggcct gaccctcgac gcccccggcg gcttctatgt cggcgtctgg ggctcggaga
      841 tcgaggagta cggtgtcggc gccgatggcg acggggcgaa gatcgaggtc gatatctcgg
      901 tcggccgggc gttcagcgcc ggcggctggg actgggacgt cgccctgttg cactacggct
      961 atcccgacgg gtcggcggtc gactatttcg agattccggt ctccgcggcg cgcaccacgg
     1021 gcgacttcac caccaccctg ggcgtggagt atgcgccgcg ccaggacaac ctcggcgacg
     1081 aggacaacct ctatctctac atcggcggcg actacgcgcc ggagagctgg ccggtcagcc
     1141 tcagcgcccg gctcggccac gaggaaggcg ccttcgccga cggcaagctc gactggcaac
     1201 tgggtgtggc caaggagctg gggccggtta gcctgacgct gcaatacacc gacagcgatg
     1261 gtccgggcgc ggagtccgcg gccgtggcgg gaatttcggt cggcttctag agcgcgttcc
     1321 gattagtggg aaccggtaat cggacaaaac gcgcgacaat aataggtctg gagcggccga
     1381 tttgattcca tcaaatcgga tcccgctctg ggcgccatga acggaaacgg gccggcctcc
     1441 gtcaggagac cggcccgtca agctgtacgc tggaagcgct cttacgaggc ctgcagctgg
     1501 cccgccgagg tcttgcccga gcggttgtcg cgctcgagtt cgtagctcag cttctggcct
     1561 tcgttcagcg aacgcatgcc ggcgcgctca acggccgaga tgtgcacaaa gacgtcggcg
     1621 ccgccgtcgt cgggctggat gaagccgtag cccttggtgg cgttgaacca cttcacggtt
     1681 ccggtagcca tggatgtaac ctctttcaac agtcaggttg cgccccgaaa ggcgcggtag
     1741 gatcgggatt tcggaaggat cgaggagcag gtccggcgca agacttcaag acgtgcggtg
     1801 gatggcgacc gaaccaggcg aaatttcgac gccgacttgc tcgcactttt tcgcagatca
     1861 ggcaagacgg cgtgagtcgg actggccacg cacggcgatc gacacccgcc ccgcggccac
     1921 cggcggcgag cgcagcaggt cgacgatctg cagcaggaat tccttgcggc gcgccagggc
     1981 gccgggcacg tcctggccgg cgcggacgaa acggacgtcc tcgcgctcgg cctcgtgggc
     2041 gacacggtcg gcgacggcgg gcaggtggcc ttcgcccttg cgggcgtcca tcagggcggc
     2101 gtgcgagcgt gcctcgtcga tccgtttgcg caccagcgcc tcgacgtcgg acccgccctc
     2161 ggcccgggcc gccagggccg cgaagtcgtg cgcatacatc cgcacgaagc cttcctcgat
     2221 catggaatct ccaatctgcc gccgaacagg gggcggccgg cgcgccatcc gcgcgcgttc
     2281 gtcgggacca tgtaacacgg attgggcgaa aacggggacg gaatgtctcc ttcgtcacgt
     2341 cccttcgggc ctgctcagga tgacgtgggc attttccacg tcatcctgag cagccgcgca
     2401 gcggcgtgtc gaaggacgaa ccacgcaagc tgccctatcc tgtctcccat gagcctgacc
     2461 caccgcctcg ccgcgccgga cgacatcccc gccctgacca ccctcatgga cgcggccatc
     2521 ggcgccctgc aggcgccgtt tctgacgccc ggtcagatcg ccgcctcgcg ccggctgatg
     2581 ggcctcgaca gccagctgat cgcggaccgg acctatttcg ttgtcgaggc ggacggcgtg
     2641 atcgccggct gcggcggctg gagccggcgg gcgaccctct atggcggcga ccacagcccg
     2701 ggccgcgacg cccatggcct gaacccggcg gaggacgcgg cccgcgtgcg cgccatgtac
     2761 acccggcccg gctt
//
LOCUS       DAORNX010000387         2769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27572_length_2769_cov_8.523213, whole genome shotgun sequence.
ACCESSION   DAORNX010000387 DAORNX010000000
VERSION     DAORNX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27572_length_2769_cov_8.523213"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLO65_14105"
     CDS             complement(<1..545)
                     /locus_tag="PLO65_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD29428.1"
                     /translation="MSAEREAAKAAFLTAHGFADARRELMSGDASTRVYERLFLADGT
                     SRIFMDQPPRAETAPCPPEATRDERIALGFNAAYRLAAGRVDAFVACARHLKSLGLSA
                     PQVEAFDATAGLAVLEDLGDDLYAARIEAGGDEAELYDAAIDALTVLHAARPPEVLAF
                     DDIAWPLLAYDDLALVSAADL"
     gene            complement(535..996)
                     /gene="tsaE"
                     /locus_tag="PLO65_14110"
     CDS             complement(535..996)
                     /gene="tsaE"
                     /locus_tag="PLO65_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRD29429.1"
                     /translation="MIPPAEIALPDEAATARLGAALARVLRDGEAICLEGPLGAGKST
                     LARALVRTLTTPDEEVPSPTFTLVQVYETAAFPLAHFDLYRLASADEAFEIGLDEALE
                     EGAALIEWPQRLEGRLPPDRLDIEIRLEDGGETRRARLTPHGAWKGRTLEC"
     gene            complement(993..1874)
                     /locus_tag="PLO65_14115"
     CDS             complement(993..1874)
                     /locus_tag="PLO65_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRD29430.1"
                     /translation="MAETATAVSVSAPPLAATGPHIVDVLIEERAPKLSRSPAWPLVR
                     PLLYRLLDYRKARAMADAIAPMDGRQALAFVSDLLRIDVAVTGLAHLPRQGRLIIVGN
                     HPTGIADGVAVYDALRATRPDIIFYANSDAHRVCPRFDETLIPVEWVPAKRTRDRTRL
                     TLAMTRQVMEADRALMVFPAGKLARRDANGVLTDPVWMPTAVSVAVKHQAPIVPVHVE
                     GPWATLFHFFDRFSPELRDITLFHELLNKRGRRFRITLGKPIAPDALESDAAVASAAI
                     KTYVERTLPFDPDRPFA"
     gene            1976..>2769
                     /locus_tag="PLO65_14120"
     CDS             1976..>2769
                     /locus_tag="PLO65_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD29431.1"
                     /translation="MRDIRHFVNGSAFEGASGRYGDIFNPNTGEVQARVQLATPAELD
                     KAVQAAAAAFPAWAATNPQRRARVMFEFKRLVEANMQELAELLSSEHGKVIADSKGDV
                     QRGLEVIEFACGIPHVLKGEYTEGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMF
                     GVAIAAGNTFILKPSEKDPSVPVRLAELMMEAGAPAGVLNVIHGDKVSVDAILQHPDI
                     KAVSFVGSSDIAHYVYSQGTLHNKRVQAMGGAKNHGIVLPDADLDQV"
BASE COUNT          439 a          956 c          983 g          391 t
ORIGIN      
        1 aacaggtcgg ccgcgctgac cagggccagg tcgtcatagg ccagcagcgg ccaggcgatg
       61 tcgtcgaacg ccagcacctc tggcgggcgg gccgcgtgca gcacggtcag ggcgtcgatg
      121 gccgcgtcgt acagttcggc ctcgtcgccg ccggcctcga tgcgcgcggc atagaggtcg
      181 tcgcccaggt cttccagcac ggccaggccg gccgtcgcgt cgaaggcctc gacctgcggc
      241 gctgacaggc ccagcgactt cagatggcgg gcgcaggcga cgaaggcgtc gacccgaccg
      301 gccgcgaggc gataggcggc gttgaagccc agggcgatcc gctcgtcccg cgtcgcctcc
      361 ggcggacacg gcgcggtctc ggcgcgcggc ggctggtcca tgaagatgcg gctcgtcccg
      421 tcggccagga acagccgttc gtagacgcgc gtcgaggcgt cgccgctcat cagctcgcga
      481 cgcgcgtcgg cgaagccgtg ggcggtcaga aaagcggcct tggcggcctc ccgctcagca
      541 ctcaagcgtc cgtcccttcc atgcgccgtg aggggtcagc cgcgcccggc gcgtctcgcc
      601 cccgtcttcg agacggatct ctatatcgag tcggtcgggc ggcagtcgcc cctccagccg
      661 ctgtggccac tcgatcagcg ccgccccctc ctccagcgcc tcgtcgagcc cgatctcgaa
      721 ggcctcgtcg gcgctggcca gccggtagag atcgaaatgg gccagcggga aggccgccgt
      781 ctcgtagacc tgaacgaggg tgaaggtcgg gctgggaacc tcctcgtccg gcgtggtcag
      841 ggtccgcacc agggcccgcg ccagggtcga cttgcccgcg cccagcgggc cttccagaca
      901 gatggcttcc ccatcccgca ggacccgcgc cagggccgcg cccagtcgcg cggtggcggc
      961 ctcgtcgggc aaggcgattt cggcgggcgg gatcatgcga aggggcggtc cggatcgaac
     1021 gggagggtcc gctccacata ggtcttgatc gccgcgctgg cgaccgccgc gtcggactcc
     1081 agggcgtcgg gggcgatcgg cttgccgagg gtgatccgga accgccgccc cctcttgttg
     1141 agcagttcgt ggaacagggt gatgtcgcgc agttccggcg agaagcggtc gaagaagtgg
     1201 aacagcgtgg cccagggccc ttccacgtga accggcacga tcggcgcctg gtgcttcacc
     1261 gccacagaca cggccgtagg catccagacc ggatcggtca gcacgccgtt ggcgtcccgc
     1321 cgggccagct tgccggccgg aaagaccatc agcgcccggt cggcctccat cacctgtcgg
     1381 gtcatggcca gggtcagccg ggtgcggtcg cgggtgcgct tggccggcac ccattccacc
     1441 gggatcaggg tctcgtcgaa ccgcgggcag acacggtggg cgtcggaatt ggcgtagaag
     1501 atgatgtccg gccgggtcgc cctgagcgcg tcatagaccg ccaccccgtc ggcgatgccc
     1561 gtgggatggt tgccgacgat gatcagccgg ccctgacgcg gcaaatgcgc caggccggtg
     1621 acggcgacat ctatcctcag cagatcagag acgaacgcca gcgcctgccg cccgtccatc
     1681 ggggcgatgg cgtcggccat cgcccgggcc ttgcgatagt cgagcaggcg gtagagcagc
     1741 ggccgcacca acggccacgc gggtgagcgc gacagcttcg gcgcccgctc ctcgatcagc
     1801 acatcgacga tatgcggccc cgtcgcggcg agcggcggcg ctgaaacgga aacggcggtg
     1861 gcggtttcgg ccatgacggg agtctatcgg cacggggccg tagcgcaatc cgacctagcc
     1921 cgatcaggcg ggaaggcata gaacgcccgc ctcaacgctc gtccgggaga tcgccatgcg
     1981 ggacatccgc catttcgtga acggttcggc cttcgaaggg gcctccgggc gctacgggga
     2041 catcttcaat cccaacaccg gcgaggtgca ggcccgggtg cagctggcga cgccggccga
     2101 gctcgacaag gcggtgcagg cggccgcggc ggccttcccg gcctgggcgg cgaccaatcc
     2161 ccagcgccgc gcgcgggtga tgttcgagtt caaacggctg gtcgaggcca acatgcagga
     2221 gctggccgag ctgctgtcca gcgagcatgg caaggtcatc gccgacagca agggcgacgt
     2281 gcagcggggc ctggaggtga tcgagttcgc ctgtggcatc ccgcacgtgc tgaagggcga
     2341 atacaccgag ggcgcgggcc cgggcatcga cgtctattcg atgcgccagc cgctgggcgt
     2401 ggtggccggc atcaccccgt tcaacttccc ggccatgatc ccgatgtgga tgttcggcgt
     2461 ggccatcgcg gcgggcaaca ccttcatcct caagccgtcg gagaaggacc ccagcgtccc
     2521 cgtccggctg gccgagctga tgatggaggc cggcgcgccc gccggcgtgc tgaacgtgat
     2581 ccacggcgac aaggtcagcg tcgacgccat cctgcagcat cccgacatca aggccgtcag
     2641 cttcgtcggc agcagcgaca tcgcccacta cgtctattcg cagggaacgc tgcacaacaa
     2701 gcgcgtccag gccatgggcg gggccaagaa ccacggcatc gtcctgcccg acgccgacct
     2761 cgaccaggt
//
LOCUS       DAORNX010000388         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27701_length_2759_cov_7.071006, whole genome shotgun sequence.
ACCESSION   DAORNX010000388 DAORNX010000000
VERSION     DAORNX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27701_length_2759_cov_7.071006"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..324
                     /locus_tag="PLO65_14125"
     CDS             94..324
                     /locus_tag="PLO65_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29432.1"
                     /translation="MIDDRSAPSFSYAVIQMGPLWRVVCARRAMGHFPSRDLALMAAS
                     SLAHEAIQAGHIAEILLQSETGELRVLWPDRP"
     gene            408..2090
                     /locus_tag="PLO65_14130"
     CDS             408..2090
                     /locus_tag="PLO65_14130"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRD29433.1"
                     /translation="MTEAVERESMEYDVVIVGGGPAGLSAAIRLKQLAEAAGTEVSVA
                     VLEKGSEVGAHILSGAVIDPKALAELFPDWKALGAPLETPVEKDRFLVLGPQGELDLP
                     MFAMPPFMHNHGCYIGSLANVCRWLGGQAEALGVEIYPGFAASDLVFREDGSVKGVVV
                     GVVGIGKDGHHKPDYQPGMELHGKYVLIGEGVRGSLAKQLEARFNLREGREPQKFGIG
                     IKELWQVPDTCFRPGLAQHTTGWPLDNNTGGGSFMYHFGDNYVTIGYVVHLNYKNPWL
                     SPFDEFQRFKHHPSVKPYLEGGRRIAYGARAITEGGWQSIPKLTFPGGALIGCSAGFV
                     NVPRIKGSHNAMKTGMLAAEEAFKALQAGREGDELTGYTTAFENSWVAKELKIVRNAK
                     PLLSKLGTFLGGAVGMFDMWVNHLTGGFSLITGTGSLKHGKTDAASTGLAKDYPQLTY
                     PRPDGVISFDKLSSVFLSATNHEEDQPAHLKLKDPSVPINVNLPLYGEPARLYCPAGV
                     YEVLYDEAGANPKFQINAQNCVHCKTCDIKDPSQNIVWTTPEGGGGPNYPNM"
     gene            2262..>2759
                     /locus_tag="PLO65_14135"
     CDS             2262..>2759
                     /locus_tag="PLO65_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29434.1"
                     /translation="MKLRFALPALAPLVLLAACATTADGPATAGLRPSQDVAFGDAPT
                     ASPFGLYLAGQAAMNDADAGAASQYFARAEQVSVDGAYFREQAFIAALMSGDIPRAAR
                     LSPSDPETRPVIQRLAALSRVVAGIGEGDGRLAVETLASGQVNVPHRPAAALLVEALR
                     ELDAEQ"
BASE COUNT          444 a          956 c          936 g          423 t
ORIGIN      
        1 gggtttcacc cgcatcggcc ggctgctgcg gtcctactac ctcgacctgg cggggctatg
       61 cgcctgcccg catagccgcg gggggagggc catatgatcg atgatcgctc cgcgccgtcc
      121 ttcagctacg ccgtcatcca gatggggccc ctgtggcggg tggtctgcgc gcggcgggcg
      181 atgggccatt ttccgagtcg ggatctggcg ctgatggccg ccagcagcct ggcccacgag
      241 gccatccagg caggccacat cgccgagata ctgctccagt ccgagaccgg cgaactgcgg
      301 gtgctgtggc ccgaccgtcc gtgagccgcc ggcaattcgc cattcctgct tgaccgcccc
      361 tgcgtcgcct tcgaataaga ccatgaggat tcaggggaag cagagccatg accgaagccg
      421 tcgaacgcga gtccatggag tatgacgtcg tcatcgtcgg cggcggcccg gccggcctgt
      481 cggcggcgat ccgcctgaag cagctggcgg aggcggccgg gaccgaggtt tcggtggcgg
      541 tgctggaaaa gggctcggaa gtcggggccc acatcctgtc gggcgcggtg atcgatccca
      601 aggccctggc cgagctgttc cccgactgga aggcgctcgg cgccccgctc gagacgccgg
      661 tggagaagga ccgcttcctg gtgctcggac cgcagggcga gctggacctg ccgatgttcg
      721 ccatgccgcc gttcatgcac aatcacggct gctacatcgg ctcgctggcc aatgtctgcc
      781 gctggctggg cggtcaggcc gaggcgctgg gcgtggagat ctatccggga ttcgcggcct
      841 cggacctggt cttccgcgag gacggctcgg tcaagggcgt ggtcgtcggc gtggtcggca
      901 tcggcaagga cggccatcac aagcccgact accagcccgg catggagctg cacggcaagt
      961 acgtgctcat cggcgaaggg gtgcgcggct cgctggccaa acagctggag gcgaggttca
     1021 acctgcgtga gggccgtgag ccgcagaagt tcggcatcgg catcaaggag ctgtggcagg
     1081 tgcccgacac ctgcttccgt ccgggcctgg cgcaacacac gacgggctgg ccgctggaca
     1141 acaacaccgg cggcggcagc ttcatgtacc acttcgggga caactacgtg accatcggtt
     1201 acgtggtgca cctgaactac aagaacccct ggctgtcgcc gttcgacgag ttccagcggt
     1261 tcaagcacca cccgtcggtg aagccctatc tcgagggcgg ccggcgcatc gcctatggcg
     1321 cccgcgcgat caccgagggc ggctggcagt cgatcccgaa gctgaccttc ccgggcgggg
     1381 cgctgattgg ctgctcggcc ggcttcgtca acgtgccgcg catcaagggc agtcacaacg
     1441 ccatgaagac cggcatgctg gccgccgaag aggccttcaa ggccctgcag gccggccgcg
     1501 agggcgacga gctgaccggc tacaccaccg ccttcgagaa cagctgggtg gcgaaggagc
     1561 tgaagatcgt ccgcaacgcc aagccgctgc tcagcaagct gggcaccttc ctcggcggcg
     1621 cggtcggcat gttcgacatg tgggtcaacc atctgaccgg cggcttctcg ctgatcaccg
     1681 ggaccgggtc cctcaagcac ggcaagacag acgccgcctc gaccgggctg gccaaggact
     1741 atccgcagct gacctatccc aggccggacg gcgtgatcag cttcgacaag ctcagctcgg
     1801 tgttcctctc ggcgaccaac cacgaggaag accagccggc gcacctgaag ctcaaggacc
     1861 cgtcggtgcc gatcaacgtc aacctgccgc tctacggcga accggcgcgg ctctactgcc
     1921 cggccggcgt ctacgaggtg ctgtacgacg aggcgggcgc caacccgaag ttccagatca
     1981 acgcccagaa ctgcgtccac tgcaaaacct gcgacatcaa ggacccgtcg cagaacatcg
     2041 tctggacgac ccccgagggc ggcggcggcc cgaactatcc gaacatgtag ttcactgggc
     2101 cgttcgacac ggcccgcggg cccgttcagg atgacgtgca ctttcgacgt catggtgagc
     2161 agcgcccgaa gagcgcgtgt cgaaccacgc accatgcttc tgactcgcct cgcgcgcact
     2221 ggcgccttgc aggcgccgca gtttgcggca aggtgggtcg catgaagctg cgtttcgcct
     2281 tgcccgccct cgctccgctc gttctgctgg ccgcctgcgc cacgaccgcc gacggtcccg
     2341 ccaccgccgg attgcggccc tcgcaggacg tcgccttcgg tgacgcgccg acggcctcgc
     2401 ccttcggcct gtacctcgcg gggcaggcgg cgatgaacga cgccgacgcc ggcgcggcct
     2461 cgcagtattt cgcgcgggcc gaacaggtca gcgtcgacgg ggcctacttc cgcgagcagg
     2521 ccttcatcgc cgccctgatg tcgggcgaca ttccccgcgc cgcgcggttg tcgccgagcg
     2581 accccgagac ccgcccggtg atccagcggc tggcggccct cagccgcgtc gtcgccggca
     2641 tcggcgaggg cgacggcagg ctggcggtcg agaccctcgc cagcggccag gtcaatgtgc
     2701 cccaccgccc ggcggcggcc ctgctggtcg aggccctgcg cgagctggac gccgagcag
//
LOCUS       DAORNX010000389         2748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27843_length_2748_cov_6.527664, whole genome shotgun sequence.
ACCESSION   DAORNX010000389 DAORNX010000000
VERSION     DAORNX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27843_length_2748_cov_6.527664"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLO65_14140"
     CDS             complement(<1..497)
                     /locus_tag="PLO65_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRD29435.1"
                     /translation="MNAKVKGMRGLFAAFAAVVMTAMAAGGVRAEELMGQPTPGGMGL
                     QPAASPLKHSAHAFHDLILLPIITVISLFVLGLLIWVIVRYNKKANPVPAKWSHNTLI
                     EVIWTLVPVLILMVIAIFSFRLLFDYHNMPKPYMTVKAVGYQWNWGYEYPDQKIPEYI
                     SAMLP"
     gene            775..1101
                     /locus_tag="PLO65_14145"
     CDS             775..1101
                     /locus_tag="PLO65_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRD29436.1"
                     /translation="MTEAIEIQLKKGVLALCVLALLSRADSYAYEIASRLAKDIDMGE
                     GTIYPLMRRLQADGLVETYLVESQSGPPRKYYRLTDAGRGSFTQQKAEWAAFTRAVDD
                     ILGAGA"
     gene            1098..1664
                     /locus_tag="PLO65_14150"
     CDS             1098..1664
                     /locus_tag="PLO65_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HRD29437.1"
                     /translation="MTRADFMARLRAGLAGLPAAAIDDIAADYETHFADAQAAGRSEQ
                     EVADALGRPDRLARELRAEAGLKKWEEQKNPSAAAAAVFALLGLGAIDILILLPILMS
                     VIGTLFALFIAAIACVIAGAAVLAAGPFFDPPGGPLTAILAGLGVMAGATSLGAVTTI
                     VTIGLVNLLVWYGRLHYRLLKPAIEPQG"
     gene            1674..2462
                     /locus_tag="PLO65_14155"
     CDS             1674..2462
                     /locus_tag="PLO65_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRD29438.1"
                     /translation="MIRNLIVIAVASFVLTVACFAGVVAIGGRELVEHGWSIPANFHV
                     DEDGDDVHISMGRDPDPGPDTTRDVAWSGGPSLEINLPADVTYSQGPVAKVTVTGPRS
                     LVDRVTIVDGALRFNQASSDARVSVGAGGGVRIDTVRRQLRITVVAPAVKRFVLNGSP
                     SLDLAGYDQPDLALEINGSGQVSGAGKTRALALAIAGSGGAELAALAAENATVSVTGS
                     GDAEVSAKGAVQVDIAGSGDVTLTTKPASLSSNIDGSGDLHLPD"
     gene            2497..>2748
                     /locus_tag="PLO65_14160"
     CDS             2497..>2748
                     /locus_tag="PLO65_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRD29439.1"
                     /translation="MTASVLAPSLLDSAGVDPDRARAILGDALAGADDGELFLERSES
                     EAFVFDDGRLKTASYDTSEGFGLRVVAGETAGYAHASEIS"
BASE COUNT          433 a          950 c          907 g          458 t
ORIGIN      
        1 tcgggaagca tggccgagat gtactcgggg atcttctggt cagggtactc gtaaccccag
       61 ttccactgat agccgaccgc cttcaccgtc atgtagggct tgggcatgtt gtggtagtcg
      121 aacagcagcc ggaacgagaa gatggcgatg accatcagga tcagcaccgg caccagggtc
      181 cagatcacct cgatcagggt gttgtggctc cacttggccg ggaccgggtt ggccttcttg
      241 ttgtagcgca cgatcaccca gatcagcagc ccgagcacga acaggctgat cacggtgatg
      301 atcggcagca ggatcagatc gtggaaggcg tgggcgctgt gcttcagcgg cgaggccgcg
      361 ggctgcagac ccattccccc gggcgtgggc tgccccatca gttcttcggc gcggacccca
      421 cccgccgcca tcgccgtcat tacgacggcg gcgaaagccg caaacagccc ccgcatcccc
      481 ttcaccttcg cgttcatgcg cctcacgtcc gtttcgccgg tcccaggtcc gccggcagtc
      541 aggagctact tgacggctgg tctggcccgc tagtgcgcgc ctttgtccca cccgactttc
      601 gccttttcag gcccggtgca tacgcgctgg aaccccgatt gccaagccgc tcaaggctgg
      661 cccggcttgg gccggccgtt gtcattgatc caaccccgct atcatgtttc aacagctacc
      721 ttgcgttagt cagtatcgtg cgatagaagg gtcccgggtt tgggaatgga actcgtgacc
      781 gaagctatcg aaatccaatt gaaaaaaggg gtgctggcgc tctgcgtcct ggccttgctg
      841 tcgcgggccg acagctacgc ctacgagatc gccagccgcc tcgcgaagga catcgacatg
      901 ggggaaggca ccatctaccc gctcatgcgc cgcctgcagg cggacgggct ggtcgagacc
      961 tatctggtgg agtcgcagag cggtccgccg cggaaatact accggctgac cgacgccggc
     1021 cgcggcagtt tcacgcagca gaaggccgag tgggccgcct tcacccgcgc cgtcgacgac
     1081 attctgggag ctggggcatg acccgcgctg acttcatggc gaggcttcgc gccggcctgg
     1141 cgggcctgcc cgcggccgcg atcgacgaca tcgccgccga ctacgagacc cacttcgccg
     1201 acgcccaggc cgcgggccgc agcgagcagg aggtcgccga cgcgctcggc cggccggacc
     1261 gccttgctcg cgaactgcgc gccgaggcag gtctgaagaa gtgggaggaa cagaagaacc
     1321 cctccgccgc cgccgccgcc gtgttcgcgt tgctgggcct cggcgcgatc gacatcctga
     1381 ttctgttgcc aattctgatg agcgtgatcg gcaccctgtt cgcgcttttc atcgccgcca
     1441 tcgcctgtgt catcgccggg gcggccgtcc tggcggcagg gcccttcttc gatccgcccg
     1501 gcggcccgct gacggccatt ctggcggggc tgggagtcat ggcgggcgcc acctccctcg
     1561 gggcggtcac gaccatcgtc acgatcgggc tggtcaacct gttggtctgg tacggccgac
     1621 tccactaccg gctgctcaag ccggccattg aaccgcaagg ctgagggctg gccatgatcc
     1681 gcaatctgat cgttatcgcc gtggcgagct tcgtcctgac ggtggcctgt ttcgccggcg
     1741 tcgtcgcgat tggcggccgg gaactggtgg agcacggctg gtccatcccc gccaatttcc
     1801 acgtcgacga ggacggggac gacgtccaca tctccatggg tcgcgatccg gacccgggcc
     1861 cggacaccac ccgcgacgtc gcgtggtcgg gcgggcccag cctcgagatc aacctgcctg
     1921 cggacgtgac ctacagccag ggtccggtcg cgaaggtgac cgtgaccgga cccaggagcc
     1981 tggtcgaccg cgtgacgatc gtggacggcg ccctgcgctt caaccaggcg tcgagcgatg
     2041 cgcgtgtcag cgttggcgcc ggcggcggcg tgcgcattga cactgtccgc aggcagctga
     2101 gaatcacggt cgtcgccccc gcggtgaagc ggttcgtgct caacggctcg ccctcgcttg
     2161 atctggccgg ctatgaccag cctgacctgg cgctggagat caacggctcc ggccaggtct
     2221 cgggcgccgg caagacccgg gccctggccc tcgccatcgc cggctccggc ggcgcggagc
     2281 tggcggccct ggcggccgaa aacgcgaccg tatcggtcac cggcagcggc gacgccgagg
     2341 tttcggccaa gggggcggtc caggtcgaca tcgccggcag cggcgacgtc accctgacga
     2401 ccaagccggc cagcctgagc agcaatatcg acggttcggg cgatcttcac ctgcccgact
     2461 gatgacggcg gcggtcgaac gccctacctg tagggcatga ccgcctccgt cctcgccccc
     2521 tcgctcctcg actccgccgg agtcgatccc gaccgcgccc gcgccatcct cggcgacgcc
     2581 ctcgccgggg ccgatgacgg ggagctgttc ctcgagcgca gcgagagtga ggcattcgtc
     2641 ttcgacgacg gcaggctgaa gaccgccagc tacgatacca gcgagggctt cggcctgcgg
     2701 gtcgtcgccg gcgagacggc cggctacgcc cacgccagcg agatctcc
//
LOCUS       DAORNX010000390         2746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27873_length_2746_cov_4.946117, whole genome shotgun sequence.
ACCESSION   DAORNX010000390 DAORNX010000000
VERSION     DAORNX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27873_length_2746_cov_4.946117"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLO65_14165"
     CDS             complement(<1..266)
                     /locus_tag="PLO65_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRD29440.1"
                     /translation="MKTLGVLGGMGPAATLDFLAKLQAATPAARDQDHIRVLVDINPQ
                     VPDRNGEDDTPGPVLAAMAAGLRDAGADVLAIACNTAHAYADAVR"
     gene            complement(397..1398)
                     /locus_tag="PLO65_14170"
     CDS             complement(397..1398)
                     /locus_tag="PLO65_14170"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HRD29441.1"
                     /translation="MNLARFARARFAHLPTPLEPAPRLSAELGIDLWIKRDDCTGLAG
                     GGNKTRKLEFLLGEALEQGADTLITQGAIQSNHVRQTAAAAASFGLACEIVLENRTGN
                     QAGDYLGSGNVLLDRLLGASIRVVPAGLDMNAEMAVNAAAARARGATPYIIPGGGSNA
                     VGALGYVDCALELAAQANDEGLVIDRLVTATGSAGTQAGLVAGMAVSGADIPVLGIGV
                     RAPKARQEDTVHGLAVETAGLLGHPERVTRDLVVADCDYVGEGYGLIDQAVIDALKLA
                     ARCEGLLLDPVYTGKAMKGLIALARQGAFAGQTVVFLHTGGAQGLFGYHGELEPALL"
     gene            1470..2030
                     /locus_tag="PLO65_14175"
     CDS             1470..2030
                     /locus_tag="PLO65_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29442.1"
                     /translation="MTPKAVLAPIVLALSLAVAAPAAPAVYVLGGGLASACSRFALDG
                     RSDDYSLNTCSYALETEVLVRRDRAATLVNRGVIKLRRKQYDSADRDFDAALRLQPEM
                     GEAFVNRGAGLIAQRRWREGVAEIDRGLALAPDEPEKAYFNRALAWEALDDMEAAWRD
                     YSQALALKPDWDAPRKELARFTVTPK"
     gene            2183..>2746
                     /locus_tag="PLO65_14180"
     CDS             2183..>2746
                     /locus_tag="PLO65_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRD29443.1"
                     /translation="MRWRGGRQSGNIEDRRGLGPVGAVGGLGLGGVVIILIGVFVFGV
                     DPQQMISVVSDGGGVSQPRQEGVRGVTSDEGGRFVGAVETSTTDVWSDIFRQSDAAYT
                     PTNGVVLYDQATNTGCGQGQAAMGPFYCPADQKIYLDLAFWNELESRFGAAGDFARAY
                     VIAHEVGHHVQDLRGELDAGQGARARGA"
BASE COUNT          372 a          982 c          996 g          396 t
ORIGIN      
        1 cgcaccgcgt cggcataggc gtgggccgtg ttgcaggcga tcgccagcac gtcggcgccc
       61 gcgtcgcgca ggcctgcggc catggccgcc agcacggggc cgggggtgtc gtcctcgccg
      121 ttgcggtcgg gcacctgggg gttgatgtcg accagcacgc ggatgtggtc ctggtcgcgg
      181 gcggcgggcg tcgccgcctg cagcttggcg aggaagtcga gggtcgcggc cggccccatg
      241 ccgcccagaa cgccgagggt cttcatggct ggtgctcgct catccgggtt ggcgtggttc
      301 gacacgcggt ctccgaccgc tgctcaccat gacgtggaaa tgcgcaattc cacgtcatcc
      361 tgagcaggcc cgaagggccg tgtcgaagga cgctcgtcac agcagcgcgg gctccagctc
      421 gccgtggtag ccgaacaggc cctgggcccc gccggtgtgc agaaacacca ccgtctggcc
      481 cgcgaaggcg ccttgccggg ccagggcgat cagccccttc atcgccttgc cggtatagac
      541 cgggtcgagc agcaggccct cgcagcgggc ggccagcttg agcgcgtcga tcaccgcctg
      601 gtcgatcagg ccgtagccct cgccgacata gtcgcagtcg gcgaccacca ggtcgcgcgt
      661 gacccgctcg gggtggccca gcaggccggc ggtctccacc gccaggccat ggacggtgtc
      721 ctcctggcgg gccttcggcg cgcgtacgcc gatgccgagc acggggatgt cggcgccgct
      781 gacggccatg ccggcgacca ggccggcctg ggtgccggcg ctgccggtgg cggtgaccag
      841 ccggtcgatc accagtccct cgtcattggc ctgggccgcc agctccaggg cgcagtcgac
      901 gtagcccagc gccccgaccg cgttgctgcc gccgccgggg atgatgtagg gcgtcgcgcc
      961 gcgggcccgc gccgccgcgg cgttgacggc catctcggcg ttcatgtcca gcccggccgg
     1021 cacgacgcgg atcgaggcgc cgagcagccg gtcgagcagc acattgcccg agccgagata
     1081 gtcgcccgcc tggttgcccg tgcggttctc cagcacgatc tcgcaggcca ggccgaagct
     1141 cgccgccgcc gccgccgtct ggcgcacatg gttggactgg atggccccct gggtgatcag
     1201 ggtgtcggcc ccctgttcga gcgcctcgcc gagcaggaac tccagcttgc gggtcttgtt
     1261 gccgccgccg gccagccccg tgcagtcgtc gcgcttgatc cacaggtcga tgcccagttc
     1321 ggcgctcagc cgcggcgcgg gctccagcgg cgtgggcaga tgggcgaagc gggcccgggc
     1381 gaacctggcg agattcatga cgcgacctga gactggattt ccgtggtatg aagactactt
     1441 caaagcgtca cgcaggggag gagaattcga tgacccccaa agccgtgctt gccccgatcg
     1501 tgctggccct gtccctggcc gtcgccgccc cggccgcccc ggccgtctac gtgctgggcg
     1561 gcggcctggc cagcgcctgt tcccgcttcg ccctcgacgg ccgctccgac gactattcgc
     1621 tgaacacctg cagctatgcg ctcgagaccg aggtgctggt ccggcgcgac cgcgccgcca
     1681 ccctggtcaa ccgcggggtg atcaagctgc ggcgcaagca gtacgactcg gccgaccgtg
     1741 acttcgacgc cgccctgcgg ctgcagccgg agatgggcga ggccttcgtc aaccgcggcg
     1801 cgggcctgat cgcccagcgg cgctggcgcg agggcgtggc cgagatcgac cggggcctgg
     1861 cgcttgcccc ggacgaaccg gaaaaggcct atttcaaccg ggccctggcc tgggaggcgc
     1921 tcgacgacat ggaggccgcc tggcgcgact acagccaggc cctggccctc aagcccgact
     1981 gggacgcccc gcgcaaggag ctggcccgct tcacggtgac cccgaagtag caggggacat
     2041 gtaccgcagg tccgtgtccc ccaaccgggc ggccgcggcc tttaggggac acggacctgc
     2101 ggcgcatgtc cctgccgccc cctctggacg gatcgtccgc ggcgccccag cgttcccttc
     2161 gggctgatca ggaggcttca tcatgcgctg gcgcggcggc aggcagtcgg gaaacatcga
     2221 ggaccggcgc gggctgggcc cggtcggggc ggtcggaggc ctgggcctgg gcggggtggt
     2281 gatcatcctg atcggggtgt tcgtgttcgg cgtcgacccg cagcagatga tcagcgtggt
     2341 cagcgacggc ggcggcgtgt cccagccgcg gcaggaaggc gtgcgcggcg tcaccagcga
     2401 cgagggcggc cggttcgtcg gcgcggtcga gacctccacc accgacgtct ggagcgacat
     2461 cttccgccag tccgacgcgg cctacacccc gaccaacggc gtcgtgctct atgaccaggc
     2521 gaccaacacg ggctgcggcc agggccaggc ggccatgggg ccgttctact gcccggccga
     2581 ccagaagatc tatctcgacc tggccttctg gaacgagctg gagagccgct tcggcgcggc
     2641 cggcgacttc gcgcgggcct atgtcatcgc ccacgaggtc ggccaccatg tgcaggacct
     2701 gcgcggcgag ctcgacgccg gccagggcgc ccgggcccgc ggggcc
//
LOCUS       DAORNX010000391         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_27928_length_2742_cov_6.995534, whole genome shotgun sequence.
ACCESSION   DAORNX010000391 DAORNX010000000
VERSION     DAORNX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27928_length_2742_cov_6.995534"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(896..1663)
                     /locus_tag="PLO65_14185"
     CDS             complement(896..1663)
                     /locus_tag="PLO65_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD29444.1"
                     /translation="MAFDGQSKTVYVAGSGPAVIVMAEMPGIGPHVARFARWVRDAGF
                     TVYMPSLFGRDGAVVSADEGAAVFRKACVSAEFRALAAGQTSPVTHWLRQLARQAHAE
                     CGGKGVGAIGMCFTGNFALSMMLEPAMLAPVLCQPALPLNDPAGLEISPEDLAAVKAR
                     LDRNQLEVLAYRFAGDKFCQAQRFAAYEQALGPRFKARVLPDSAAHFADAPPFFQTHV
                     PFAHSVVTVHLVDKEGEPTVAAREEIMGFLAGQLGRY"
     gene            complement(1754..2674)
                     /locus_tag="PLO65_14190"
     CDS             complement(1754..2674)
                     /locus_tag="PLO65_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD29445.1"
                     /translation="MTAHPRDFQVVTADQLLIGAREWRHDGDASGRNVVVIAPATSVR
                     ARYYHRFAEYLHASGCDVVTFDYRGIGASRPLGGLRRFNASYVDWGRYDLEAVLQYVT
                     SRYPGQPIDVVAHSIGGFTLGLAASSHRVRRALTVGAQIAYWPDYNPRKRLQMVLRWH
                     LFMPLVAWLFGYFPGARLGWLEDTPLGIVRQWSALHRDFEKQPWKTHRTDPPLPLRFE
                     LFRGDTLAISLADDDWGTPAAIRRLLGLYKHARRHHLHLEPADIGVSQIGHFAYFNSR
                     FADSLWPLALRWLQTGAMPAPFAARLCAVG"
BASE COUNT          503 a          760 c          971 g          508 t
ORIGIN      
        1 gcccctgagc tttcacgttg ggcgacaaaa actgaagggg cggatgaatg acagatcgac
       61 gaagcgaagt gccagtatgg gcatggctga acgcacaaat aattcaagag gcatttcgat
      121 ctggcgactt gaacagagca acgcgcaaaa tcgaaaaatc gtataagcgc gctcggataa
      181 gttcttcgat cttcagaggc tcctagccta ggaaaagcgt gcctcaaaac acgaaagact
      241 ttttgctgac cgcttagtct ccctcccaat tcattgaggg ttgtcaatct tggctacctc
      301 gtttctcttc tctattgcgc tatcgtcgta ccgaaggaac tgtgggcaac tgacaaactg
      361 cctattgcgc tcgcgaagat cgatgcgaag tggctactca gtctcatatc gatatcagtc
      421 aaatccgcaa attttgatca ggatcccgtc aatcgctttt tcagacatct tcgaaatgcg
      481 gtcgcacatg ttcgatttga ggtcaatgat caaggcggct tcacgttttg ggatcaacaa
      541 aacgaaaatt caccacaaag cttccgcgca agtttgctgc aaagttcact cgaagagttc
      601 ctgtccaaag ttggcccagc acttgcctat ttgcattttc agcatgatcg tgtgcactga
      661 gttgtcgccc aaccttacgt tcgagcggga cgctgcaaaa gcgcggcgcc cctcaacttt
      721 acgttgggcg tccgctttcc aagacttcga ctgtccgctt cgggtcggga gtgtgaattc
      781 gtcagatcga taagctgccg ttgaagtcga tttccgactc gatggctggt aatcggcgca
      841 ttgcggacct ccaagcacgg agactcgtac ggccggattg gccgaatagt tgccctcagt
      901 accgccccaa ctgcccagcc aagaacccca ttatctcctc ccgcgccgcc accgtcggtt
      961 ccccttcctt gtccaccaga tgcaccgtca ccacgctatg cgcgaagggg acgtgcgtct
     1021 ggaagaaggg cggggcgtcg gcgaagtggg cggcggagtc gggtagcacg cgggccttga
     1081 agcgggggcc gagggcctgt tcgtaggcgg cgaagcgttg ggcctggcag aacttgtcgc
     1141 cggcgaagcg gtaggcgagg acttcgagct ggttgcggtc gaggcgggct ttgacggcgg
     1201 cgaggtcttc gggggagatt tcgaggccgg ccgggtcgtt gaggggtagc gcgggctggc
     1261 agaggaccgg ggcgagcatg gccggttcca gcatcatcga cagcgcgaag ttgccggtga
     1321 agcacatgcc gatggcgccg acgcccttgc cgccgcattc ggcgtgggcc tgccgggcga
     1381 gctggcgcag ccagtgggtt accgggctgg tctggccggc ggccagggcg cggaattcgg
     1441 cgctgacgca ggccttgcgg aagacggcgg cgccttcgtc ggcggagacg accgcgccgt
     1501 cgcggccgaa cagcgagggc atgtagacgg tgaagccggc gtcgcgcacc cagcgggcaa
     1561 agcgggcgac gtgcgggccg atgccgggca tctcggccat gacgatgacg gccgggccgc
     1621 tgccggcgac gtacacggtc ttgctctggc cgtcgaaggc catgtcgcgt cgcgagaagt
     1681 ccttgagcgg gtcgtcgtgc agcaggtggt ggtagggtat ggcgttctcc ttgggttggt
     1741 gttcgggcgg ggttcagcca accgcgcaca ggcgcgcggc gaagggggcg ggcatggcgc
     1801 cggtctgtag ccagcgcagg gcgagcggcc acaacgagtc ggcaaaccgg ctgttgaagt
     1861 aggcgaaatg gccgatctgg ctgacgccga tgtcggcggg ttcgaggtgc aggtggtggc
     1921 gtcgggcgtg tttgtagagg ccgagcaggc ggcggatggc ggccggggtg ccccagtcgt
     1981 cgtcggccag gctgatggcc agggtgtcgc cgcggaacag ctcgaaccgc aggggcagtg
     2041 gcgggtcggt gcggtgggtt ttccagggct gcttttcgaa atcgcggtgc agggcgctcc
     2101 actggcggac gatgccgagc ggggtgtctt cgagccagcc gaggcgggca ccgggaaagt
     2161 agccgaacag ccacgccacg agcggcatga agaggtgcca gcggagcacc atctgcaggc
     2221 gtttgcgagg gttgtagtcc ggccagtagg cgatctgggc gccgacggtc agggcgcggc
     2281 gcacgcgatg gctggaggcg gcaaggccga gcgtgaagcc gccgatgctg tgagcgacga
     2341 cgtcgatggg ctggccgggg tagcggctgg tgacgtactg gaggacggct tcgagatcgt
     2401 accggcccca gtcgacatag ctggcgttga agcggcgcag gccgcccagc gggcgcgagg
     2461 cgccgattcc gcgatagtcg aaggtgacga catcgcagcc gctggcgtgg aggtattcgg
     2521 cgaagcggtg gtagtagcgc gccctgaccg aggtggcggg agcgatgacg acgacgttgc
     2581 ggccgctggc atcgccgtcg tggcgccatt cgcgggcgcc gatcagcagc tggtcggcgg
     2641 tgacgacctg gaagtcgcgg gggtgtgcgg tcatggcgtt ctccttggct agcagcctgt
     2701 cggactttac ccttgccgcc cgctgaatat gctaaaatca ta
//
LOCUS       DAORNX010000392         2735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28028_length_2735_cov_9.658209, whole genome shotgun sequence.
ACCESSION   DAORNX010000392 DAORNX010000000
VERSION     DAORNX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28028_length_2735_cov_9.658209"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            944..1468
                     /locus_tag="PLO65_14195"
     CDS             944..1468
                     /locus_tag="PLO65_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29446.1"
                     /translation="MKTGAFWRNINPHRRTTPARVVKGLGAGALAGAALVTGAYALMF
                     LSAGSLAYFPLLLLVFPVALVVWAVGLCAFAGPGWWILHMIGARSPFASMAYGGTLCT
                     LLGMAVALLLPLSIAPADQGHKVLSTPITETGWLGVAPGVLLFAATGVAVGWVVHRVA
                     YGRQMQAAGADSQT"
     gene            complement(1527..>2735)
                     /gene="pnp"
                     /locus_tag="PLO65_14200"
     CDS             complement(1527..>2735)
                     /gene="pnp"
                     /locus_tag="PLO65_14200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRD29447.1"
                     /translation="ILDTGIRIDGRTVDKVRQIVSEVGVLPRAHGSALFTRGETQALV
                     VATLGTGDDEQLIDALEGKYYEKFMLHYNFPPYSVGETGRMGAPSRRDVGHGKLAWRA
                     IRPMLPDAVDFPYTIRLVSEITESNGSSSMATVCGSSLALMDAGVPLKKPVSGIAMGL
                     ILEKDGFAVLSDILGDEDHLGDMDFKVAGTEDGITSLQMDIKIAGITEAIMKQALAQA
                     KAGREHILGEMSKAMEAPRAELGEFAPKIETIKIPVDKIREVIGTGGKVIREITAETG
                     AKIDIGEDGTIKIAAADQAKIDAAKEWIKSIASEPEIGAIYNGKVVKVVDFGAFVNFF
                     GAKDGLVHVSQISNERVSRPADVLTEGQMVKVKLLGFDDRGKTKLSMKVVDQETGEDL
                     SRKEEASAEA"
BASE COUNT          407 a          887 c          936 g          505 t
ORIGIN      
        1 cgaacgccgc caccaggccg ccgcgcagcc gcgccgccag cggacgcgac caggcccagg
       61 cgaccagctc catcgccatc tccagccccg accgtcgcat cgcccgcgcc attcacaatc
      121 cccgattccg agaccgcccg ttgtcgcgcg gagagggtta aggggcgttt actttggggt
      181 ggttcggaga cggttcccga cagagtgcgg ttaccgaaaa cagcgcatgg ctacgcggcc
      241 tccggggaca cgcactgaag tgcatgtccc cgtgggtgag gagggcgcgg cgcgctccgg
      301 cctcagcggg gccgaggtat gggtatctgg tctgagaagg ccggggccgt cgcgcgccgc
      361 gaagttcgtt ttccgggagc cgccggtggg cagtggtttt agcacttagc cggcagaagc
      421 ctccggcggg cgggtgatgg ctgatcatcc ccggaccgcg acggtaaccc cccgtcgcct
      481 taccccgctc gaactccacc ccgccgccgc atcggatcgc ctggccgaga cgccctcccc
      541 gtgcggcggg atggggtgag tatggcggcg gcgcgacggg gggatgggag gattgttcgg
      601 cgcggtcgga tagcgcgggc ggaggtgagg gatttcaggg ggttgggtga ggggacctga
      661 agtttccccc cggagaatcc tctcctccgg gagagaaagt tcaccgatga gagtccgccc
      721 tgccgccttg atggaatggg agcgcggccg gtcgccctgg tcaaggacga cccctgcggg
      781 cccgccgctg cgcggccgcg aagcggtcct tgagcaggac gtccgcccgc gcaatgctcc
      841 gccaaggcat cagggcgggg tgtggccgtc acgatacaaa gcgcctagag caaatccgaa
      901 tgcccgacgg tgaactgggc acagcgctgg agacaccacc tggatgaaaa ccggcgcgtt
      961 ctggcgaaat ataaaccctc atcggcggac cacccccgcg agagttgtga agggccttgg
     1021 cgccggcgcg cttgcgggcg ccgccttggt aactggcgcc tatgcgctca tgtttctgtc
     1081 ggccggaagc ctcgcctact ttccgctttt gctgttggtt ttcccggtcg cgctggtcgt
     1141 ctgggcagta gggctctgtg cctttgccgg tccagggtgg tggatccttc atatgatcgg
     1201 cgcgcgaagt ccgttcgcca gcatggcgta tggcggaacc ctctgcactt tgctgggaat
     1261 ggcggtcgcc ctgttgctgc ccctgtcgat cgccccggca gatcagggac acaaagtcct
     1321 atcgaccccc atcactgaga ctggctggtt aggtgtggcg ccaggcgtcc ttctgttcgc
     1381 cgccactggc gtggcggtag ggtgggttgt ccaccgggtg gcctatgggc gacagatgca
     1441 ggctgcgggc gctgactcgc agacatagaa aaagggcggc cccgtttcca gagccgcccc
     1501 aattcaatca gcgatgctga agcgtcctac gcctcggcgg aggcctcttc cttcctggac
     1561 aggtcttcgc cggtttcctg gtcgacgacc ttcatcgaca gcttggtctt gccgcgatcg
     1621 tcgaagccga ggagcttgac cttgaccatc tggccttcgg tcagcacgtc ggccgggcgc
     1681 gagacccgct cgttgctgat ctggctgacg tggaccaggc cgtccttggc gccgaagaag
     1741 ttcacgaagg cgccgaagtc gacgaccttg accaccttgc cgttgtagat ggcgccgatc
     1801 tcgggctcgg aggcgatcga cttgatccac tccttggccg cgtcgatctt ggcctggtcg
     1861 gcggccgcga tcttgatcgt gccgtcctcg ccgatgtcga tcttggcgcc ggtttcggcg
     1921 gtgatctcgc ggatcacctt gccgcccgtg ccgatcactt cgcggatctt gtcgaccggg
     1981 atcttgatcg tctcgatctt cggggcgaac tcgcccagct cggcgcgcgg cgcttccatg
     2041 gccttggaca tctcgcccag gatgtgctcc cggccggcct tcgcctgggc cagggcctgc
     2101 ttcatgatcg cctcggtgat gccggcgatc ttgatgtcca tctgcaggct ggtgatgccg
     2161 tcctcggtgc cggcgacctt gaagtccatg tcgcccaggt ggtcctcgtc accgaggatg
     2221 tccgacagga ccgcgaaacc gtccttctcg aggatcaggc ccatggcgat gccgctgacc
     2281 ggcttcttca ggggcacgcc cgcatccatc agcgccagcg acgagccgca gaccgtggcc
     2341 atcgaggacg agccgttgga ctcggtgatc tcggacacca gacggatggt gtaggggaag
     2401 tcgaccgcgt ccggcagcat cgggcggatc gcccgccagg ccagcttgcc gtggccgacg
     2461 tcgcggcggc tcggcgcgcc catgcggccc gtctccccga ccgagtaggg cgggaagttg
     2521 tagtgcagca tgaacttctc atagtacttg ccctcgaggg catcgatcag ctgctcgtcg
     2581 tcgccggtgc ccagggtggc cacgaccagg gcctgggttt cgccacgggt gaacagggcc
     2641 gagccgtggg cgcggggcag gacgcccact tcgctgacga tctggcggac cttgtcgacc
     2701 gtgcggccgt cgatgcggat gccggtgtcg aggat
//
LOCUS       DAORNX010000393         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28158_length_2726_cov_6.083489, whole genome shotgun sequence.
ACCESSION   DAORNX010000393 DAORNX010000000
VERSION     DAORNX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28158_length_2726_cov_6.083489"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1074
                     /locus_tag="PLO65_14205"
     CDS             121..1074
                     /locus_tag="PLO65_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HRD29448.1"
                     /translation="MSDGRAGIEAQAVGLAEAVARQRKATVVVKRIAWTGRVGRLPWW
                     LNLFPRRWLTPESAFEPPWPDLVIAAGRATLPLSIRLRRWSGRKTWVVQIQDPRAPAQ
                     LFDLVVPPNHDRLKGDNIVSIIGAPHRVTPKKLADEYKRFAAAIDPLPHPRAAVLIGG
                     RSRAFDLSPERASAIAQQIQLALEQEGGSLLMTFSRRTPPQAKALLTARLKHLPGIIW
                     DGEGDNPYFAFLAGADYVLVTEDSANMATEAAGTGKPVFILKMDGQSLKFRLFHEELE
                     RHGAARPFGGAFHGWTYEPLAETDRAAGEILRRMDARAASS"
     gene            1197..1916
                     /locus_tag="PLO65_14210"
     CDS             1197..1916
                     /locus_tag="PLO65_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRD29449.1"
                     /translation="MILAILQARMSSTRLPGKVLMPLAGAPMIVRQIERVARARRIDR
                     LVVATSLNPGDDAIEKVVRKEGIAVHRGSLDDVLARFIGALDEHGPADHVVRLTADCP
                     LADPFVIDETIETLLETDADYASNTPPHRTFPKGLDVEVMTAETLRRAAATATTPEER
                     EHVTWGIWNHPDRYRLAFHSQPADEGEVRWTVDRPDDYAFMAAVYDALYRENRAFTSD
                     AVRRFVSSRPDLATYGGGRRV"
     gene            1913..2482
                     /gene="pseH"
                     /locus_tag="PLO65_14215"
     CDS             1913..2482
                     /gene="pseH"
                     /locus_tag="PLO65_14215"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HRD29450.1"
                     /translation="MTAPTVTLRDVTPDDSARILAWRNSEAVRSYMYTDHLISPDEHA
                     RWFAGIAGDERRRYWIIELDARPVGLANLYDIDRRHGRCAWAYYLADPAVRGLGLGSY
                     VEFWIIEQVFGPQGLTKLWCEVLASNEAVWRLHEKHGFVQEARFRRHIVKDGAGVDVL
                     GLGLLAEEWAGRRGEMAARLQEKGFSLPA"
BASE COUNT          425 a          989 c          930 g          382 t
ORIGIN      
        1 aggacccgag cacacccgtg tctgacggtg ttcatgggtg gcccggacaa gccgggccat
       61 gacgagtatt agggcgacgt ggcgaagcaa ccggcaaacc ctcccctgac gatctgggcc
      121 gtgtccgacg gacgggccgg gatcgaggcc caggccgtcg gcctggccga ggcggtggcg
      181 cgccagcgca aggcgacggt ggtcgtcaag cgtatcgcct ggaccggccg cgtcggccgc
      241 ctgccctggt ggctgaacct gttcccccgc cgctggctga cccccgaaag cgcgttcgag
      301 ccgccctggc cggacctggt gatcgccgcc ggccgcgcca ccctgcccct gtcgatacgc
      361 ctgcgccgat ggtccggccg caagacctgg gtggtgcaga tccaggatcc ccgggccccg
      421 gcccagctat tcgacctggt cgtgccgccc aaccatgacc gcctcaaggg cgacaacatc
      481 gtctcgatca tcggcgcgcc gcaccgcgtg acgccgaaga agctggccga cgagtacaaa
      541 cggttcgccg cagccatcga ccccctgccc cacccgcgcg cggcggtgct gatcggcggc
      601 cgctcgcggg cgttcgacct gtcgcccgag cgcgcctcgg ccatcgccca gcagatccag
      661 ctggcgctcg agcaggaggg cggctcgctg ctgatgacct tctcgcgccg cacgccgccg
      721 caggcgaagg ccctgctgac cgcccggctc aagcatctgc ccgggatcat ctgggacggc
      781 gagggcgaca acccctattt cgccttcctt gccggggccg actatgtgct ggtcaccgag
      841 gactcggcca acatggccac cgaggccgcc ggcacgggca agccggtgtt catcctcaag
      901 atggacggcc agagcctgaa gttccgcctg ttccacgagg agctcgaacg ccacggcgcg
      961 gcccgcccgt tcggcggcgc cttccacggc tggacctacg aacccctggc cgagaccgac
     1021 cgggccgcgg gggagattct ccggcggatg gacgcgcggg cggccagttc ctgaaccgtc
     1081 atcgtcggac ttgttccgac gacccatgaa cgccatcgtc ctgacgaagg gtgcttcgtt
     1141 tgcggcgccg agtgccaggt gcccgggatg agcccgggga tgacggagaa acgggtttga
     1201 tcctcgccat cctccaggcc cgcatgagct ccacccggct gccgggcaag gtgctcatgc
     1261 cgctggccgg ggcgccgatg atcgtgcggc agatcgagcg ggtcgcccgc gcgcggcgca
     1321 tcgacaggct ggtcgtggcc accagcctca accccggcga tgatgcgatc gagaaggtcg
     1381 tgcgcaagga gggcatcgcc gtccaccgcg gctcgctcga cgacgtgctg gcccggttca
     1441 tcggcgcgct ggacgagcat ggtccggccg atcatgtcgt gcgcctgacc gccgactgtc
     1501 cgctggccga cccgttcgtg atcgacgaga ccatcgagac cctgcttgag accgacgccg
     1561 actacgccag caacaccccg ccccaccgca cctttcccaa gggcctcgac gtcgaggtga
     1621 tgaccgccga gaccctgcgc cgggccgccg cgacggcgac cacgcccgag gagcgcgagc
     1681 acgtcacctg gggcatctgg aaccaccctg atcgctatcg cctggcgttc cactcccagc
     1741 cggccgacga gggcgaggtg cgctggaccg tcgaccggcc cgacgactac gccttcatgg
     1801 ccgccgtcta cgacgcgctc taccgggaga accgggcctt cacctcggac gcggtgcgcc
     1861 ggttcgtgtc atcgcgcccc gacctggcga cctatggcgg aggccgccgc gtatgaccgc
     1921 cccgaccgtc accctgcgcg acgtcacgcc cgacgacagc gcccgcatcc tggcctggcg
     1981 caacagcgag gcggtgcggt cgtacatgta caccgaccac ctgatcagcc cggacgagca
     2041 cgcccgctgg ttcgccggca tcgcgggcga cgagcgacgg cgctactgga tcatcgaact
     2101 ggacgccagg ccggtgggcc tggccaacct ctacgacatc gaccggcggc acggccgctg
     2161 cgcctgggcc tactatctgg ccgacccggc cgtgcggggc ctgggcctgg gcagctatgt
     2221 cgagttctgg atcatcgagc aggtgttcgg cccacagggg ctgaccaagc tgtggtgcga
     2281 ggtgctggcc agcaacgagg cggtctggcg gctgcacgag aagcacggct tcgtgcagga
     2341 ggcccggttc cggcggcaca tcgtcaagga cggcgccggc gtggacgtgc tgggcctggg
     2401 actgctggcc gaggagtggg ccgggcgaag gggcgagatg gcggcgaggt tgcaggagaa
     2461 ggggttttcc ttacccgcgt aacatcaacc cttgtcatcc cggacagccg taaggctgat
     2521 ccgggaccta gatcaaatct ctgccgaatc tggggcccgg ctctccgctg cgctgcggcc
     2581 gggatgacag tggatgcgcg ttgagcacta accggacaag gccagcagcg cctccgccgc
     2641 gcggtccgcg cccagcccgt cgcacagggc cgccgaggcg gccgacaacc ggtcccgcag
     2701 accagcatcc gccaccagcc ggctca
//
LOCUS       DAORNX010000394         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28220_length_2722_cov_9.330709, whole genome shotgun sequence.
ACCESSION   DAORNX010000394 DAORNX010000000
VERSION     DAORNX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28220_length_2722_cov_9.330709"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLO65_14220"
     CDS             complement(<1..121)
                     /locus_tag="PLO65_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRD29451.1"
                     /translation="MHLPRLPDWLVYLAIVAALVIASMGRREQADAPPPPPPMA"
     gene            complement(128..556)
                     /locus_tag="PLO65_14225"
     CDS             complement(128..556)
                     /locus_tag="PLO65_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRD29452.1"
                     /translation="MPPQLQSMEIQAFSTGFVTTLGHAGLTLVILLLGAVLYAMLTPH
                     REIALIRDGNNAAALSLGGVLIGLALPLASSLSASVSPLEIAIWGAATVAVQLLVFRG
                     IDLILHGLPQRIQDGEISAAVLLVGAKVASALILAAALAG"
     gene            complement(623..1783)
                     /gene="rlmN"
                     /locus_tag="PLO65_14230"
     CDS             complement(623..1783)
                     /gene="rlmN"
                     /locus_tag="PLO65_14230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRD29453.1"
                     /translation="MGATLNLARATPVVAAAPANLSGLTREGLARAFVEAGAVPADKA
                     KMRATQVFRWIHHRGVTDFAQMTDVAKDTRAALAEHFTLGRPEVVERQVSRDGTRKWL
                     IRTAPGIEIEAVYIPDVGRSGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVAQV
                     QVCKDDLEEWPSDREDRRLSNIVFMGMGEPLYNLDNVSDAIDIISDNEGIGISRRRIT
                     VSTSGVVPELKALGERTQAMLAISLHATDDALRDVLVPINRKYDIAALMDGIRAYPGI
                     SNARRVTFEYVMLKGVNDTPEEALALVRLLKGIPAKINLIPFNPWPGTQYECSDWATI
                     ERFAAILNKAGYSSPIRTPRGRDILAACGQLKSESEKVRASVRRKAEGLASG"
     gene            1873..2430
                     /locus_tag="PLO65_14235"
     CDS             1873..2430
                     /locus_tag="PLO65_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD29454.1"
                     /translation="MTAPESDPYADLMNRVASHGDRAAYAALFEHYAPRVKGYLGRLG
                     LEPGRADDLAQEVMVAVWRKAASFDRRKATVSTWIYRIARNRRIDLFRRERTATLDPE
                     EPQLSPAALPLPDADLEAIQREAQVAAALADLPPEQREMVRAAFYEDLTHSEIAERFG
                     LALGTVKSRLRLAFEKLRGRLESAR"
     gene            2501..>2722
                     /locus_tag="PLO65_14240"
     CDS             2501..>2722
                     /locus_tag="PLO65_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29455.1"
                     /translation="MIAPPPLPDPASHPGLRLMEATAAVLRGAQAAPRPADVVGGAFL
                     EGETPVPLSDGAADQVLARIAGLEAIDARA"
BASE COUNT          406 a          919 c          989 g          408 t
ORIGIN      
        1 cggccatcgg cggcggcggg ggcggcgcgt cggcctgctc gcgacgcccc atcgaggcga
       61 tcaccagcgc cgccacgatc gccagataga ccagccagtc gggaagtcgg ggcagatgca
      121 cggggtctca gccggccagg gccgcggcga ggatcagggc gctggccacc ttggcgccga
      181 ccagcagcac cgcggcggag atctcgccgt cctggatgcg ctgcggcagg ccgtgcagga
      241 tcaggtcgat gccacggaac accagcagct gcacggccac cgtggccgcg ccccagatgg
      301 cgatttccag cggtgagacc gaggcgctca gcgacgaggc cagcggcagg gcgaggccga
      361 tcagcacgcc gcctagcgac agggccgcgg cgttgttgcc gtcgcggatc agggcgatct
      421 cgcgatgggg cgtcagcatg gcgtagagca ccgcgccaag cagcaggatg accagcgtga
      481 gtccggcgtg gccgagggtg gtgacgaagc ctgtcgagaa ggcctggatt tccatcgact
      541 ggagttgcgg cggcatcggg acgtccatgc gggaacttag ggcgcaaggg ttagcacgac
      601 cggcgccatt ccacgcaagc ttctagcctg aagcgagccc ctcggccttg cggcggaccg
      661 atgcgcggac cttctcgctc tccgatttca gctgcccgca ggcggcgaga atgtcgcggc
      721 cgcggggcgt gcggatcggg ctggaatagc cggccttgtt gaggatcgcc gcgaaccgct
      781 cgatggtcgc ccagtcggag cattcgtact gtgtgcctgg ccagggattg aacgggatca
      841 ggttgatctt ggccgggatg cccttgagca gccggaccag ggccagcgcc tcctcgggcg
      901 tgtcgttgac gcccttgagc atgacgtatt cgaacgtcac ccgccgggcg ttgctgatcc
      961 cgggataggc gcggatgccg tccatcaggg cggcgatgtc atacttcctg ttgatcggca
     1021 cgagcacgtc gcgcagggcg tcgtcggtgg cgtgcaggct tatcgccagc atcgcctggg
     1081 tgcgctcgcc cagcgccttc agctcgggga ccacgccgct ggtcgagacc gtgatgcggc
     1141 ggcgggaaat gccgatgccc tcgttgtcgc tgatgatgtc gatggcgtcg gagacgttgt
     1201 cgagattgta cagcggctcg cccatgccca tgaagacgat gttcgacaga cgccggtcct
     1261 cgcggtccga cggccattcc tccagatcgt ccttgcagac ctgcacctgc gcgacgatct
     1321 cggccgccgt caggttgcgc accagggcct gggtgccggt gtggcagaag gtgcagttga
     1381 gcgtgcagcc gacctggctg gagacgcaaa gggcgcccga gcgaccgacg tcggggatgt
     1441 agaccgcctc gatctcgatg cccggcgccg tgcggatcag ccatttgcgc gtgccgtccc
     1501 gtgacacctg ccgctcgacg acctcgggcc ggcccagggt gaagtgttcg gccagggccg
     1561 cgcgggtgtc cttggcgaca tcggtcatct gggcgaagtc ggtcacgccg cgatggtgga
     1621 tccagcggaa cacctgggtg gcgcgcatct tcgccttgtc ggccggcacg gcgccggcct
     1681 cgacgaaggc gcgcgccagt ccctcgcggg tgagacccga aaggttggcc ggggcggcgg
     1741 cgacgacggg cgtcgcgcga gcaagattga gggtcgcgcc caagacaggg ggttccgatg
     1801 cggtgagggg agtccccata tcagaaaacc ccgctctgga ccaatcggag cgggcgtgac
     1861 tatcccttgg cggtgaccgc gcccgagagc gacccctacg ccgacctgat gaaccgcgtc
     1921 gccagccacg gcgaccgggc ggcctatgcg gcgttgttcg aacactacgc gccccgggtg
     1981 aagggctacc tgggccggct gggcctggag ccgggccggg ccgacgacct ggcccaggag
     2041 gtgatggtcg cggtctggcg caaggccgcc agcttcgatc gccgcaaggc cacggtgtcg
     2101 acctggatct accgcatcgc ccgcaatcgc cgcatcgacc tgttccgccg cgaacgcacg
     2161 gcgacgctcg atcccgaaga gccgcagctg agccccgccg ccctgcccct gccggatgcg
     2221 gacctcgagg cgatccagcg cgaggcgcag gtcgccgcgg ccttggccga cctgccgccc
     2281 gagcagcgcg agatggtgcg cgccgccttc tatgaggacc tgacgcacag cgagatcgcc
     2341 gagcggttcg gcctggcgct gggcacggtc aagtcgcgtc tgcggctggc gttcgagaag
     2401 ctgcgcggtc ggctggagtc cgcgcgatga gccggcgccc cgccttgacc ccggatcgat
     2461 ccggtccata tcccgccttt tcaaggagtt gcccggccgc atgatcgccc cgccccccct
     2521 tccggacccc gccagccatc ctggcctgcg cctgatggaa gcgacggcgg ctgtcttgcg
     2581 cggcgcgcag gcggcgcccc ggccggccga cgtagtcggc ggcgctttcc tcgagggcga
     2641 gacgcccgtg ccgctgtccg acggggcggc cgaccaggtt ctggcccgta tcgccggtct
     2701 cgaggcgatc gacgcccggg cg
//
LOCUS       DAORNX010000395         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28315_length_2716_cov_6.335964, whole genome shotgun sequence.
ACCESSION   DAORNX010000395 DAORNX010000000
VERSION     DAORNX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28315_length_2716_cov_6.335964"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /gene="ilvB"
                     /locus_tag="PLO65_14245"
     CDS             complement(<1..1268)
                     /gene="ilvB"
                     /locus_tag="PLO65_14245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRD29456.1"
                     /translation="MTASATRTAPTDAAAREMTGAEIVIQALIDQGVSTLFGYPGGAV
                     LPIYDALFHDNARHGDRLRHILVRHEQGATHAAEGYARSTGKPGVVLVTSGPGATNAI
                     TGIVDALMDSIPMVVFTGQVPTHLIGTDAFQEADTVGITRACTKHNYLVKDVADLPRI
                     IHEAFHIATSGRPGPVLIDIPKDVQFSRGLYRSPDEVTFSHGYAPRTKGDPARIAEAA
                     KLIAKARRPIFYTGGGVINAGPAASESLRAFAAMTGAPVTSTLMGLGAFPAADPAWLG
                     MVGMHGTFEANNALHDCDVMVCIGARFDDRVTGRLDAFSPGSKKIHVDIDASSINKNV
                     RVDIGIVGDAGSVLDDLIAAWKAGGYGPNRNALDDWWRQIDGWRARQCLSYRTRPDTI
                     KPQQAIERLYALTRDRDTYITTEVGQHQMWA"
     gene            complement(1445..2380)
                     /gene="miaA"
                     /locus_tag="PLO65_14250"
     CDS             complement(1445..2380)
                     /gene="miaA"
                     /locus_tag="PLO65_14250"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRD29457.1"
                     /translation="MSKQILLIAGPTASGKSALALRAARAFGGEIVNADALQLYADLR
                     ILSARPTDAEQADIPHHLFGVADGADGWSVGRWLRAAAPTIGDIIARGRTPVVVGGTG
                     LYFKALTEGLADIPPTPAAVRQGVAAMYDTLGEDRFRSLLAGADPDAALRIEAGDRLR
                     LQRAMEVMKATGRPLSAWQADTTPALGPDAWRAVAIVPDRAELYARCDARFDAMLTGG
                     ALDEVRALLARRLDPRLPVMKAVGVRELAACLAGETTLDRAADLARQETRRYAKRQLT
                     WLRNQTPDWPRITEIGTDAAWRQFLALEPALTGLT"
BASE COUNT          379 a          906 c         1019 g          412 t
ORIGIN      
        1 gcccacatct ggtgctggcc gacctcggtg gtgatgtagg tgtcgcggtc ccgggtcagg
       61 gcgtagagcc gctcgatggc ctgctgcggc ttgatcgtgt ccggtcgggt ccggtagctc
      121 aggcactggc gggcccgcca gccgtcgatc tgtcgccacc agtcgtccag ggcgttgcgg
      181 ttcgggccat agccgccagc cttccaggcc gcgatcaggt cgtccagcac gctgccggcg
      241 tcgccgacga tgccgatgtc gacccgcacg ttcttgttga tcgaggaggc gtcgatgtcg
      301 acgtggatct tcttgctgcc cggcgagaag gcgtccagcc ggcccgtcac gcggtcgtcg
      361 aaacgggccc cgatgcagac catcacatcg cagtcgtgca gggcgttgtt ggcctcgaag
      421 gtgccgtgca tgccgaccat gcccagccag gccgggtcgg ccgccgggaa cgcgcccaag
      481 cccatcaggg tcgaggtcac cggtgcgccg gtcatggcgg cgaaggcccg caggctctcg
      541 ctggccgccg ggccggcgtt gatcaccccg ccgccggtgt agaagatcgg ccggcgggcc
      601 ttggcgatga gcttcgcggc ttccgcgatg cgggccgggt cgcccttggt gcgcggggcg
      661 tagccgtggc tgaaggtcac ctcgtccggc gagcggtaga ggccgcgcga gaactgcacg
      721 tccttgggga tgtcgatcag caccgggcca ggccggccgg aggtcgcgat gtggaaggcc
      781 tcgtggatga tccgcggcag gtcggcgacg tccttcacca ggtagttgtg cttggtgcag
      841 gcgcgggtga tgccgaccgt gtcggcctcc tggaaggcgt cggtcccgat caggtgggtc
      901 ggcacctggc cggtgaagac gaccatcgga atgctgtcca tcagggcgtc gacgatgccg
      961 gtgatggcgt tggtcgcgcc cgggcccgat gtgaccagca cgacgcccgg cttgccggtc
     1021 gaacgggcgt agccctcggc ggcatgggtc gcgccctgct cgtgccggac caggatgtgg
     1081 cgcaggcggt cgccgtgacg ggcgttgtcg tggaacaggg cgtcatagat cggcaacacc
     1141 gccccgcccg gatagccgaa cagggtgctg accccctggt cgatcagggc ctggataacg
     1201 atctcggcgc cggtcatctc gcgggcggcg gcgtcggtcg gggcggtccg ggtggcggag
     1261 gcggtcatgg caggctcttc ggggggacga aaaaggcaat aaaaaaggcc cgtaggggcc
     1321 tgggatgcgc gcgaccggcg acaacgtgac ggatcaggtc acgctgttgt cggccgcccg
     1381 gcaagtacga tgatcgtgtt gcgcacgaag attgctccaa atcgcaggac ttggagatgc
     1441 cacgctacgt gaggcccgtc aaggcgggtt caagcgcaag gaattgcctc cacgccgcat
     1501 cggttccgat ttcggtgatc cgcggccagt ccggcgtctg gttgcgcagc caggtcaact
     1561 ggcgcttggc gtagcggcgg gtctcctggc gggccaggtc ggcggcccgg tcgagggtcg
     1621 tttcgccggc caggcaggcg gccagctcgc gcacgccgac agccttcatc accggcagcc
     1681 ggggatcgag gcggcgcgcc agcagggcgc gaacctcgtc aagcgctccg ccggtcagca
     1741 tggcgtcgaa ccgggcgtcg cagcgggcgt agagttcggc ccggtcggga acgatggcca
     1801 cggcgcgcca ggcgtcgggt ccgagggccg gggtcgtgtc ggcctgccag gcgctcagcg
     1861 ggcggccggt ggccttcatc acttccatcg cccgttgcag tcgcaggcga tcgccggcct
     1921 cgatccggag cgcggcgtcg ggatcagcgc cggcgagcag gctgcggaag cggtcttcgc
     1981 cgagggtgtc gtacatcgcc gccacgccct ggcgcacggc ggccggcgtc ggcggaatgt
     2041 cagcgaggcc ctccgtcagg gccttgaagt agaggccggt gccaccgacc acgaccggag
     2101 tccggccacg cgcgatgatg tcgccgatgg tgggagcggc ggcccgcagc cagcggccga
     2161 cggaccagcc gtctgcgccg tccgcgacgc cgaacaggtg atgggggatg tcggcctgct
     2221 cggcgtccgt cggccgcgcc gacaggatcc gcagatcggc atagagctgc agcgcgtcgg
     2281 cgttgacgat ctcgccaccg aacgcccgcg ccgcccgcaa ggccagggcg gacttgccgc
     2341 tcgcggtcgg cccggcgatc agcaggatct gtttcgacat gctccccgct taaggcaagc
     2401 agcgccggtt cgccaccggg gcagcgggga gcctcgtgcg tccttcgaca cgccgctccg
     2461 cggctgctcg ggatgacgtg ggttcgcgcc gcggcactcg tccacgtcat ggtgagcagc
     2521 gctcgacgag cgcgcgtcga accacgcaac ccggttccca tcctcgcgaa agcaggctag
     2581 gcggcgcgca tgcagctcgt cgtcaccctc gtcgccgccg acccccacga cctcgtggcc
     2641 gccctggagg cggtggagat cgccgtggcc ggcacgggcg cgaccgtcgg cgagccggac
     2701 cttctgggcc ccagcg
//
LOCUS       DAORNX010000396         2713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28363_length_2713_cov_6.652370, whole genome shotgun sequence.
ACCESSION   DAORNX010000396 DAORNX010000000
VERSION     DAORNX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28363_length_2713_cov_6.652370"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLO65_14255"
     CDS             <1..717
                     /locus_tag="PLO65_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRD29458.1"
                     /translation="GGLHALPMQVVSPEWVLVLLAREHPDQTLTAAEGRRAVHAGQDS
                     RFYRPIRAGDLLAVSATLLGARASRAGVVTAIRFDTRDARTGELVVETLSTTVYRDVT
                     LTGKEAPDVPSPAYQAPAGDAPTVPIPTSAGLPHIYSECAEIWNPIHTERQVALAAGL
                     PDIIIHGTALWALAGLQAARLHADGDTRRLTRLAARFTGQAFPGQPMTLAHDRDGAGV
                     RLALHSDDGRVLLTGLAEFR"
     gene            complement(736..1803)
                     /gene="rutA"
                     /locus_tag="PLO65_14260"
     CDS             complement(736..1803)
                     /gene="rutA"
                     /locus_tag="PLO65_14260"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HRD29459.1"
                     /translation="MEIGVFIPIGNNGWLISETSPQYMPSFALNKTITQAADRYGFDF
                     ALSMIKLRGFGGKTQFWEHNLESFTLMAGLAAVTERIKIFATAATLTLPPAIVARMAA
                     TIDSIAPGRFGINLVTGWAEAEYSQMGLWPGDSHYTRRYDYLAEYAQVLRDLFDTGVS
                     NFKGDYFQMTDCRVSPQAKDTKIICAGSSTEGLAFTAQYADYSFALGKGTNTPTAFAD
                     VNERLRAAKAKTGRDVSAYILFMIIADETDEKAMAKWELYRDGVDREAVAWLVGQAAG
                     NTSEDTNTRQLAAEKAAVNLNMGTLVGSYESIARMLDEVATVPDTAGVLLTFDDFVRG
                     VEDFGTKIQPLMTCRAHVKPG"
     gene            complement(1818..2630)
                     /gene="rutD"
                     /locus_tag="PLO65_14265"
     CDS             complement(1818..2630)
                     /gene="rutD"
                     /locus_tag="PLO65_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HRD29460.1"
                     /translation="MPRSGTADGLYYELHDAGPDDPREVVVLSAGLGGSGAFWAPQMA
                     ALTARFRVLLYDHRGTARSARTLRAGHRVSHMADDIVAVLDDLGLAKVHLVGHAAGGL
                     AGLALALDHPDRLDRLVVVNGWAAPDPHVARVFATRLRLLNDSGPEAYVHAQPLFLYP
                     ADWISENHDRLALEEPHHVAGFPTPEVMLARIQALLDFDIDARLPEITAPVLVSASAD
                     DTLVPVSCSRRLAARLPHATLDIVPWGGHGFTVTAPDAFNASLVAFLGGETR"
BASE COUNT          397 a          912 c          988 g          416 t
ORIGIN      
        1 ggcggtctgc acgccctgcc gatgcaggtg gtgtcgcccg aatgggtgct ggtgctcctg
       61 gcgcgcgagc atccggacca gaccctgacg gccgccgaag gccgccgggc cgtccatgcc
      121 ggccaggaca gccgcttcta ccggccgatc cgcgccggcg acttgctggc ggtgtcggcc
      181 accctgctcg gcgcgcgagc cagccgggcc ggcgtggtga ccgcgatccg cttcgacacc
      241 cgcgacgcca ggaccggtga gctggtggtc gaaaccctgt cgaccaccgt ctatcgcgac
      301 gtgaccctga ccgggaagga ggcgccggac gtgccctcgc cggcctatca ggcgccggcc
      361 ggagacgcgc cgacggtccc gatcccgacc agcgcgggcc tgccgcacat ctattcggaa
      421 tgcgccgaga tctggaaccc gatccatacc gagcgacagg tggcgctggc cgcgggcctg
      481 ccggacatca tcatccacgg aaccgcgctc tgggccctcg caggcctgca ggcggcgcgg
      541 cttcatgcgg acggcgacac gcggcggctg acccgcctgg cggcccgctt cacgggccag
      601 gccttccccg gacagccgat gaccctggcg catgaccgcg atggcgcggg cgttcgcctg
      661 gcgctgcatt cggacgatgg gcgggtgttg ctgacgggtc tcgccgagtt caggtgacgg
      721 ggcgtcctgc cgggctcaac ccggtttgac gtgcgcccgg caggtcatca gcggctggat
      781 cttcgttccg aaatcctcga ccccccgcac gaagtcgtcg aaggtcaaca gcacgccggc
      841 cgtgtccggc accgtggcca cctcgtccag catccgcgcg atggattcat acgagcccac
      901 cagggtgccc atgttgagat tcaccgccgc cttctcggcc gccagctgcc tggtgttggt
      961 gtcctcggaa gtgttgccgg ccgcctggcc gaccagccag gccaccgcct ccctgtcgac
     1021 gccgtcgcgg tagagctccc acttggccat ggccttctcg tcggtctcgt cggcgatgat
     1081 catgaacagg atgtaggcgc tgacgtcgcg gccggtcttc gccttcgcgg cgcgcagacg
     1141 ctcgttgacg tcggcgaagg cggtcggggt gttggtgccc ttgcccaggg cgaagctgta
     1201 gtcggcgtat tgcgcggtga aggccaggcc ctcggtcgag gagccggcgc agatgatctt
     1261 ggtgtccttc gcctgcgggc tgacccggca gtcggtcatc tggaagtagt cgcccttgaa
     1321 gttcgacacc ccggtgtcga acaggtcgcg cagcacctgg gcgtattcgg cgaggtagtc
     1381 gtagcggcgg gtatagtggc tgtcgcccgg ccacaggccc atctggctgt actcggcctc
     1441 ggcccagccg gtgaccaggt tgatgccgaa ccggcccggc gcgatggagt cgatggtcgc
     1501 ggccatgcgc gcgacgatgg ccggcggcag cgtgagtgtg gcggctgtcg cgaagatctt
     1561 gatccgctcg gtgaccgcgg cgaggcccgc catcagggtg aagctctcga ggttgtgctc
     1621 ccagaactgc gtcttcccgc cgaagccgcg cagcttgatc atcgacaggg cgaagtcgaa
     1681 gccgtagcgg tccgccgcct gggtgatcgt cttgttcagc gcgaacgagg gcatgtactg
     1741 cggcgaggtc tcggagatga gccagccgtt gttgccgatc gggatgaaga cgccgatttc
     1801 catgggaagt ccttgggtta gcgcgtttcg ccgccgagga aggcgacgag tgaggcgttg
     1861 aaggcgtccg gcgcggtcac ggtgaagccg tggccgcccc aggggacgat gtcgagggtg
     1921 gcgtggggca ggcgcgcggc cagccgccgc gagcagctga ccggcacgag ggtgtcgtcg
     1981 gccgaggcgc tgaccagcac cggcgcggtg atctccggca gacgcgcgtc gatgtcgaaa
     2041 tcgagcaggg cctggatgcg cgccagcatc acctcggggg ttgggaaacc ggcgacgtga
     2101 tggggctcct ccagcgccag ccggtcatgg ttttcgctga tccagtcggc cggatagagg
     2161 aacagcggct gcgcgtgaac ataggcctcc gggccgctgt cgttgagcag gcgcaggcgc
     2221 gtggcgaaca cccgcgccac gtgcgggtcc ggcgcagccc agccattgac caccaccagc
     2281 cggtccagcc ggtcgggatg gtcgagcgcc agcgccagtc cggccagccc gccggccgcg
     2341 tgaccgacga ggtggacctt cgccagcccc aggtcgtcca gcacggcgac gatgtcgtcg
     2401 gccatgtggc tcacacggtg gcccgcgcgc agggtccgcg cgctgcgggc cgttccccgg
     2461 tgatcgtaga gcaggacgcg gaagcgcgcg gtgagggccg ccatctgcgg cgcccagaac
     2521 gcgcctgacc caccgaggcc ggcggacagc acgacgacct cgcggggatc gtccgggccc
     2581 gcgtcatgga gctcgtagta gaggccgtcg gcggtcccgg agcggggcaa ggctcagcgc
     2641 ttgccgatgt gggcgatgga cgcgatctcg accaggaagt cgggccgcac caggccgcag
     2701 acgatgcaga acc
//
LOCUS       DAORNX010000397         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28386_length_2711_cov_5.595633, whole genome shotgun sequence.
ACCESSION   DAORNX010000397 DAORNX010000000
VERSION     DAORNX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28386_length_2711_cov_5.595633"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..>2711
                     /gene="alaS"
                     /locus_tag="PLO65_14270"
     CDS             213..>2711
                     /gene="alaS"
                     /locus_tag="PLO65_14270"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRD29461.1"
                     /translation="MKSLNDIRSAFLDYFGSNGHAVTPSAPLVPQNDPTLLFVNAGMV
                     PFKNLFTGAETPTALRAASSQKCVRAGGKHNDLDNVGYTARHLTFFEMLGNFSFGDYF
                     KEEAITHAWTLLTRDFGMNKDRLSVTVYHTDDEAAALWRKIAGLPEERIIRISTNDNF
                     WSMGDTGPCGPCSEIFYDHGDHIWGGPPGSADEDGDRFVEIWNLVFMQFEQHADGTRT
                     NLPKPSIDTGMGLERFATVLQGVHNVYDVDLFKTLIAASEEATGVKAEGERTPSHRVI
                     ADHLRTSSFLIADGITPSNEGRGYVLRRIMRRAMRHAHLLGATEPLMHRLAPTLVEQM
                     GAAYPELRRAQPAIVETLRQEEERFRRTLGRGMILLDEATAGLTAGGVLPGDVAFKLH
                     DTYGFPLDLTQDAVRALDLTVDTAEFDRLMDEQRGKSEGLKGTGQRAAAAEWFAIRDR
                     VGGTVFTGYDEVDGSGTVVALLGDGVEVAEAGAGDTVQAVFDRTPFYAESGGQAGDVG
                     VVDWAGGSARVTDVQKQAGDLHVHDLRIVSGSLKTGQAVTLHVDPANRTTTRANHSAA
                     HLMHAALRNVLGEHVTQKGQMVDGERLRFDFSHGGPLTADALARIEDEVNAVVLQNRA
                     AETRMMAPQQAIEAGAMALFGEKYGDEVRVLTLGRSLAGEGAYSVELCGGTHVARTGD
                     IGLFKIVSEGGIAAGVRRIEALTGEAARRWLQEQANVARDLADRFKVPVAEVPQRIEA
                     LDAQRRKAEKDLAEAKKTLALGGGGAGPVAAEDIGGVSFIGKVLDGVGGKELRGLAEE
                     FRKQAGVVALVGAADGKAAIAVAVSPELTGRFNAA"
BASE COUNT          430 a          996 c          923 g          362 t
ORIGIN      
        1 ccatcgggac gtatcggcat cgcccgtcga atggccggcg ggcgtcggcg cggccaccgg
       61 gccgggccat ccgcgagggc ccgccgccgc cgcaattccc gccgccagcg accttttcct
      121 ttgtgaccac gccgcccctt ggctaatgag cccgttcaat tcacatccgg cgacggcgcc
      181 cgcgccccgt cgggcagcag accggccgac cgatgaagag tctcaacgac atccgcagcg
      241 ccttcctcga ctatttcggg agcaacggcc atgccgtgac cccttccgcg cccctggtgc
      301 cgcagaacga tccgaccctg ctgttcgtca acgccggcat ggtgccgttc aagaacctgt
      361 tcaccggcgc cgagaccccc accgccctgc gcgcggcctc gtcgcagaag tgcgtccgcg
      421 cgggcggcaa gcacaatgac ctggacaacg tcggctacac cgcccgccac ctgaccttct
      481 tcgagatgct cggcaacttc tcgttcggcg actacttcaa ggaagaggcg atcacccacg
      541 cctggaccct gctgacccgc gacttcggca tgaacaagga ccggctgtcg gtcacggtct
      601 accacaccga tgacgaggcg gcggccctgt ggcgcaagat cgccggcctg ccggaagagc
      661 ggatcatccg catctcgacc aacgacaact tctggtcgat gggcgacacc ggtccgtgcg
      721 gcccctgttc cgagatcttc tacgatcacg gcgaccacat ctggggcggt cctccgggca
      781 gcgccgacga ggacggcgac cggttcgtgg agatctggaa cctggtcttc atgcagttcg
      841 agcagcatgc cgacggaacg cggaccaacc tgcccaagcc gtcgatcgac accggcatgg
      901 ggctggagcg gttcgccacc gtcctgcagg gcgtgcacaa cgtctatgac gtcgacctgt
      961 tcaagaccct gatcgccgcc tccgaggaag ccaccggggt gaaggccgag ggcgagcgga
     1021 ccccgtcgca ccgggtcatc gccgaccacc tgcgcacctc gtccttcctc atcgccgacg
     1081 gcatcacccc gtcgaacgag ggacgcggct acgtcctgcg ccggatcatg cgccgggcca
     1141 tgcgtcacgc ccatctgctg ggcgcgaccg agccgctgat gcaccgcctg gccccgaccc
     1201 tggtcgagca gatgggcgcc gcctatcccg agcttcgccg cgcccagccg gccatcgtcg
     1261 agaccctgcg ccaggaggag gagcggttcc gccgcaccct cggccgcggc atgatcctgc
     1321 tcgacgaggc caccgccggc ctgaccgccg gcggggtgct gccgggcgac gtggccttca
     1381 agctgcatga cacctacggc ttccccctcg acctgaccca ggacgcggtg cgggccctgg
     1441 acctgaccgt cgacaccgcc gaattcgacc ggctgatgga cgagcagcgc ggcaagtcag
     1501 agggcctcaa gggcacgggc cagcgggccg cggccgccga gtggttcgcc atccgcgacc
     1561 gggtcggcgg caccgtcttc accggctacg acgaggtcga cggaagcgga accgtcgtcg
     1621 ccctgctcgg cgatggcgtg gaagtcgccg aggccggcgc cggcgacacg gtccaggcgg
     1681 tgttcgaccg cacccccttc tacgccgaga gcggcggcca ggccggcgat gtgggcgtgg
     1741 tcgactgggc tggcggctcg gcccgcgtga ccgacgtgca gaagcaggcc ggcgacctgc
     1801 acgtgcacga cctgcggatc gtctcgggca gcctgaagac cggccaggcc gtgaccctgc
     1861 acgtcgatcc ggccaaccgc accaccacgc gcgccaacca ctctgccgcc cacctgatgc
     1921 acgcggccct gcgcaacgtg ctgggcgagc acgtgaccca gaagggccag atggtcgatg
     1981 gcgagcggct gcgcttcgac ttcagccacg gcggcccgct gaccgccgac gcgctcgccc
     2041 gcattgagga cgaggtcaat gccgtggtgc tgcagaaccg cgcggcggag accaggatga
     2101 tggccccgca gcaggccatc gaggccggcg ccatggccct gttcggcgag aaatacggcg
     2161 acgaggtgcg cgtgctgacg ctcggccgca gcctggccgg cgagggggcc tattcggtgg
     2221 aactgtgcgg cggcacccat gtggcccgca ccggcgacat cggcctgttc aagatcgtca
     2281 gcgagggcgg catcgccgcc ggcgtgcgcc gcatcgaggc cctgaccggc gaagcggcgc
     2341 gtcgctggct gcaggagcag gccaatgtcg cccgcgacct ggccgatcgc ttcaaggtcc
     2401 cggtggccga ggtgccgcag cgcatcgagg cgctcgacgc ccaacgccgc aaggccgaga
     2461 aggacctggc cgaggcgaag aagaccctcg ccctgggcgg cggcggggcg ggccccgtgg
     2521 cggccgagga catcggcggc gtcagcttca tcggcaaggt gctcgacggc gtcggcggca
     2581 aggaactgcg cggcctggcc gaggaattcc gcaagcaggc cggcgtggtg gccctcgtcg
     2641 gcgcggccga cggcaaggcg gccatcgccg tggccgtctc gcccgagctg accggccggt
     2701 tcaacgccgc c
//
LOCUS       DAORNX010000398         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28504_length_2701_cov_6.292895, whole genome shotgun sequence.
ACCESSION   DAORNX010000398 DAORNX010000000
VERSION     DAORNX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28504_length_2701_cov_6.292895"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLO65_14275"
     CDS             <1..509
                     /locus_tag="PLO65_14275"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRD29462.1"
                     /translation="WPDGPADRAGLKVGDVILAVGGETVNDEGGATYQFGSRRPGESV
                     PVRILRDGDERSVTVRAEPAPGGKDERTISGANPFQGATVASMSPAVADEIGVDPFAA
                     RGVVVTRVAKGFALQAGLKPGDLVREVNGREIRSMGDLNAAIGGGGKVWRVTIQRGGQ
                     LITAAFRT"
     gene            622..1926
                     /locus_tag="PLO65_14280"
     CDS             622..1926
                     /locus_tag="PLO65_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRD29463.1"
                     /translation="MSDLFEAAGLTPHAPAPLAERLRPRALDEVVGQDHLLGEGGPIR
                     RMIAGGRLGSMILWGPPGTGKTTIARLLAKAAGYEFQQISAVFSGVADLKKAFEQARM
                     RRAAGQSTLLFVDEIHRFNRAQQDGFLPFVEEGTITLVGATTENPSFELNGALLSRCQ
                     VYVLRRLDEAAMEQLLQRAEAEEGTTLPLELAAREAMIAMADGDGRYLLTLAETLLAI
                     GSAHPLTPAELGQVLQKRSPAYDKDREEHYNLISALHKSVRGSDPDAALYWLARMLEG
                     GEDPLFIARRLTRMASEDIGAADPMSLLLCNAAKDTYDFLGSPEGELALAQACVHMAC
                     APKSVAVYKAFGAARKAARETGSLAPPSHILNAPTRLMKDLGYGAGYAYDPDTEEGFS
                     GQNYFPDSMERRGFYKPKGEGHEAKVKERLDRWAAMRAKKNV"
     gene            2100..>2701
                     /locus_tag="PLO65_14285"
     CDS             2100..>2701
                     /locus_tag="PLO65_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRD29464.1"
                     /translation="MSSRPPRKTKAHEKPIALTPEEIAFVRGMVIAQDPHIIALNKPA
                     GLSSQGGRINAHTLDELTSAFARPGGHRPRLVHRLDRDTSGVILTARTQPAASFLGKA
                     MIAKRFRKTYLAIVAPGAPSPKGGVIDSPLRRESIGREAYMRVSAPDHPDAETAKTRY
                     RTLAATPEAALMELSPETGRMHQLRVHMASLGRPIVGDVR"
BASE COUNT          426 a          983 c          923 g          369 t
ORIGIN      
        1 tgtggcccga cggaccggcc gaccgggcgg ggctcaaggt cggcgacgtg atcctggccg
       61 tcggcggcga gacggtgaac gatgagggcg gcgccaccta ccagttcggc tcgcgccggc
      121 ccggcgagtc ggtgccggtg cggatcctgc gcgacggcga cgagcgcagc gtcaccgtgc
      181 gggccgaacc ggccccgggt ggcaaggacg agcggaccat ctcgggcgcc aaccccttcc
      241 agggcgcgac cgtggccagc atgtccccgg ccgtggccga cgagatcggc gttgatccgt
      301 tcgcggccag gggcgtggtg gtgacccgcg tggccaaggg cttcgccctg caggccggcc
      361 tcaagcccgg tgacctggtg cgagaggtca acggccgcga gatccgctcg atgggcgacc
      421 tcaacgccgc catcggcggg ggcggcaagg tctggcgcgt gaccatccag cgcggcggcc
      481 agctgatcac ggccgcgttc aggacgtagg ggacatgtac cgcaggtacg tgtcccccaa
      541 tcgaaccgcc agtgctgcgg aagcgtgatt tggggacacg tacctgcggt acatgtcccc
      601 gtctgatgct aaaccctccc catgtccgac ctgttcgagg ccgccggcct gactccccac
      661 gctcctgcgc cgcttgccga gcggctgcgg ccgcgcgcgc tggacgaggt tgtcggtcag
      721 gaccacctgc tcggcgaggg cgggccgatc cggcgcatga tcgccggcgg ccggctgggc
      781 tcgatgattc tctggggccc tccgggcacg ggcaagacca ccatcgcccg gttgttggcg
      841 aaggccgccg gctatgagtt ccagcagatc agcgcggtct tctccggcgt cgccgatctg
      901 aagaaggcct tcgaacaggc caggatgcgc cgcgccgcgg gccagtcgac cctgctgttc
      961 gtcgacgaga tccaccgctt caaccgcgcc cagcaggacg gcttcctgcc gttcgtggag
     1021 gaggggacca tcaccctggt cggcgccacc accgagaatc ccagcttcga gctcaacggc
     1081 gccctgctgt cgcgctgcca ggtctatgtg ctgcgccggc tcgacgaggc ggccatggag
     1141 cagctgctgc agcgggccga ggcggaggag gggaccaccc tgccgctcga gctggcggcg
     1201 cgcgaggcga tgatcgccat ggccgacggc gacggccgct atctgctgac cctggccgag
     1261 accctgctgg ccatcggctc cgcccatccg ctgaccccgg ccgagctcgg ccaggtgctg
     1321 cagaagcgca gcccggccta cgacaaggac cgcgaggagc actacaacct catcagcgcg
     1381 ctgcacaaaa gcgtgcgcgg cagcgatccg gacgcggccc tctactggct ggcgcgcatg
     1441 ctcgagggcg gcgaggaccc gctgttcatc gcccgccgcc tgacccgcat ggccagcgag
     1501 gacatcggcg cggccgatcc gatgagcctg ctgctgtgca acgccgcgaa ggacacctac
     1561 gacttccttg gcagccccga gggtgagctg gccctggccc aggcctgcgt gcacatggcc
     1621 tgcgcgccca agtcggtggc cgtctacaag gccttcgggg cggcccgcaa ggcggcccgc
     1681 gagaccggct ccctggcccc gcccagccac atcctcaacg ccccgacccg cctgatgaag
     1741 gacctcggct acggggccgg ctacgcctac gacccggaca ccgaggaggg cttctcgggc
     1801 cagaactact tcccggacag catggaacgg cgcgggttct acaagccgaa gggggagggg
     1861 cacgaggcca aggtgaagga gcgcctggac cgctgggcgg cgatgcgggc gaagaaaaat
     1921 gtctgacctc tccctcttgg gagaggggga ccatgcgcag catggtggag tgggatgcgc
     1981 cgctgtgagc cgtcttcgct cacgcctctc agcgtcttcc cgaatgcccc ttcgaacagc
     2041 cgtgcttccc tctccaccag cctccggctg gtccccctct cccaagtggg agaggtagca
     2101 tgtcctctcg cccgccccgc aagaccaagg cccacgaaaa gccgatcgcc ctcacgccgg
     2161 aggagatcgc cttcgtccgc ggcatggtga tcgcgcagga cccccacatc atcgccctga
     2221 acaagccggc aggcctttcc agccagggcg ggcggatcaa cgcccatacg ctcgatgaac
     2281 tgacctcggc cttcgcgcgg ccgggcggcc accggccccg gctggtccac cggctggacc
     2341 gcgacaccag cggggtgatc ctcaccgccc gcacccagcc ggccgcaagt tttctcggca
     2401 aggccatgat cgcgaagcgt tttcgcaaga cctacctggc catcgtcgca cccggggcgc
     2461 cgtcgccgaa gggcggggtg atcgacagcc cgctgcgccg cgaaagcatc ggccgcgagg
     2521 cctacatgcg cgtctcggcc ccggaccatc ccgacgccga gaccgcgaaa acccgctacc
     2581 gcaccctggc cgccacgccg gaggcggcgc tgatggagct gagtcccgag accggccgca
     2641 tgcaccagct gcgcgtgcac atggccagcc tcggccggcc gatcgtcggc gacgtccgct
     2701 a
//
LOCUS       DAORNX010000399         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28649_length_2690_cov_9.445541, whole genome shotgun sequence.
ACCESSION   DAORNX010000399 DAORNX010000000
VERSION     DAORNX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28649_length_2690_cov_9.445541"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLO65_14290"
     CDS             <1..189
                     /locus_tag="PLO65_14290"
                     /inference="COORDINATES: protein motif:HMM:NF023363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HRD29465.1"
                     /translation="AIPWLLLTARGDGSAGRLGRVASIQRVNTTGGTAPESGCDGSRS
                     GQIGRVPYTADYLFYARP"
     assembly_gap    263..392
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <393..1416
                     /locus_tag="PLO65_14295"
     CDS             <393..1416
                     /locus_tag="PLO65_14295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRD29466.1"
                     /translation="VRFLASSLWPNGTAPAAADAAVAAANRAVLLKAVPAQKDAIESA
                     YRTALASIADADARNAGIAIGERAAGTVLNARGSDGAGSAESYRPHTTPGAYVPTAIP
                     AVPQWAGRRPWMLDSPAQFRPAAPPALTSATWARDFEEVKTLGAKGSTQRNEEQTAIA
                     RFWEFSMPAIYHDLVASVATRPGRDVLANARLYAVVAQAMDDALISVMDAKYHYNFWR
                     PVTAIRNGDVDGHPATVRDAAWSPLIDTPAHPEYPCAHCILAATVGTVLKSEMAPGMV
                     LAAASPTAQGATRRWTSTDSLMREVADARIFDGVHYRNSTEVGLAMGQRIGELAAARL
                     RAPVVD"
     gene            1552..1770
                     /locus_tag="PLO65_14300"
     CDS             1552..1770
                     /locus_tag="PLO65_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29467.1"
                     /translation="MKKVKPALTPAFRKLAVKRGKAAAKGTPNGVGNRRMTPEERKLP
                     RLQAEGGRLKRALEIVMKAAASFTKDTL"
     gene            1870..>2690
                     /locus_tag="PLO65_14305"
     CDS             1870..>2690
                     /locus_tag="PLO65_14305"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRD29468.1"
                     /translation="MGGAAPATDGPAEGALIPAIPAELQGADGRPRKGREGRRRGFPA
                     SQERIERGRRENGLHARHPRRDQVTTDSRHARPVAENRWARQSIPTAPHPLGTSDIPS
                     WWTEEGGRYPAIVLDWFHREVVGGSLKPRMTAELGTDALTMAGFRRRPAPGLRHHADR
                     GSPYASPAFRDALKEYGMVCSMSRQGNGGDHAPTESGFNRFKHGWGHGVRPASPAAMK
                     ATRFEDIEVVYNRKRQPSSLGYLSPVQFMEQWLGRQNQEKQVAGTPPLGRRITEG"
BASE COUNT          457 a          869 c          881 g          353 t
ORIGIN      
        1 gccatcccgt ggctgctgct gacagcccgc ggcgatggct cggccggccg cctcggccgc
       61 gtggcctcga tccagcgggt caataccacg ggcggtacgg cgccggagag cggatgcgac
      121 ggctcccgca gcgggcagat cggccgggtt ccctacaccg ccgactacct cttctacgcg
      181 cggccctgac cgcccgcaaa tttattgaaa ggaaacatca tgaccaagct tgacctgatc
      241 ccctcccgcc gcacgctgtg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtgaggt tcctggcctc gagcttgtgg
      421 ccgaacggca ccgccccggc ggccgccgac gccgccgttg ccgcggccaa ccgggccgtg
      481 ctcctgaaag ccgttcccgc gcagaaggac gccatcgagt cggcctatcg cacggccctg
      541 gcgagcattg ccgatgcgga cgcccgcaat gccggcatcg ccatcggcga gcgggccgcg
      601 gggaccgtgc tcaacgcacg gggaagcgac ggcgctggca gcgcggaaag ctatcggccc
      661 cacaccaccc ccggcgccta cgtgccgacg gcgatcccgg cggtaccgca gtgggccggc
      721 cggcgcccgt ggatgctcga ttcgcccgcg cagttccgcc ccgcggcgcc acccgccctg
      781 accagcgcca cctgggcccg cgacttcgag gaagtgaaga ccctcggcgc caaaggcagc
      841 acacaacgga acgaagagca gacggccatc gcgcgcttct gggaattctc gatgccggcc
      901 atctaccacg acctcgtcgc ctcggtggcg acgcgccccg gccgcgacgt gctcgccaat
      961 gcgcggctct acgccgtcgt ggcacaggcg atggacgacg cgctgatcag cgtgatggac
     1021 gccaagtacc actacaactt ctggcgcccg gtgacggcaa tccgcaacgg cgacgtcgac
     1081 ggccatccgg caaccgtgcg cgacgccgcc tggtccccct tgatcgatac gccggcgcat
     1141 ccggaatacc cctgcgccca ctgcattctc gcggcgacgg tgggcacggt gctcaagtcc
     1201 gagatggcgc caggaatggt actcgccgcc gccagcccca ccgcccaggg cgcaacgcgc
     1261 cgctggacga gcaccgatag tctgatgcgc gaagtggccg acgctcggat ctttgacggc
     1321 gtgcactacc gcaactcgac cgaagtcggc ctcgccatgg gccagcgcat cggcgaactg
     1381 gcggccgcac gcctgcgcgc cccggttgtc gattgagccg gaagccggga gccggtcttt
     1441 cctttttcct gccgatttgt gtcgcagtcg catacaggaa tcacagtgaa cttcccccga
     1501 aatttcgtct tcctagggtg acggatgatt cagtcacttg aggagcgcgc aatgaagaaa
     1561 gtgaagccgg ccttgacacc ggcgttcagg aaactggcgg tcaagcgggg gaaggccgcg
     1621 gcgaagggga cgcccaacgg ggtgggcaac cggaggatga cgcccgaaga gaggaaactg
     1681 ccgcggctgc aagccgaggg cgggcgactc aagcgggcgc ttgaaatcgt aatgaaagcg
     1741 gcggcgtcct tcacgaagga taccctgtga agtccgcctg gattgacgtg cttcggcgag
     1801 agttcggggg ggcggaacgg tgcgcggcct tgaaggggag tatccgcggt gaccgggcct
     1861 ggaagcgggg tgggtggcgc agcgccagcc acggacggac ccgcagaggg ggcgctgatc
     1921 ccggccattc ctgcagaact ccagggcgcc gatggccgcc cacgcaaggg gcgcgaaggg
     1981 cgaaggcgtg gtttcccggc gagccaggag cggatcgagc gggggaggcg cgagaacggg
     2041 cttcacgccc gtcacccgcg ccgcgaccag gtgacgacgg attcgcgcca cgcccggccg
     2101 gtggccgaga accggtgggc tcgccagagc atccccacgg caccccatcc gctcgggacc
     2161 tcggacatcc cgtcttggtg gacggaggaa ggcgggcggt acccggccat cgtgctggat
     2221 tggttccacc gggaagtcgt gggcgggtca ctcaagcccc gcatgacggc agaactcggg
     2281 acggacgccc tgaccatggc ggggttccgc cgccggccgg ccccgggtct gaggcatcac
     2341 gccgaccgcg gtagcccgta cgccagccct gcgtttcggg acgcgctcaa ggagtacggc
     2401 atggtctgtt cgatgagccg ccagggcaat ggcggggatc acgcgcccac cgagagcggg
     2461 ttcaaccgct tcaagcatgg gtggggacat ggcgtacgtc ctgccagccc tgccgccatg
     2521 aaggccaccc gctttgaaga catcgaggtg gtttacaatc ggaaacgcca gccgtcgagc
     2581 ctgggctacc tgtcgccggt tcagttcatg gaacagtggc tcggcaggca gaatcaggaa
     2641 aaacaggtcg caggaacacc acccttggga agacgaataa ccgagggaag
//
LOCUS       DAORNX010000400         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28692_length_2687_cov_7.469225, whole genome shotgun sequence.
ACCESSION   DAORNX010000400 DAORNX010000000
VERSION     DAORNX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28692_length_2687_cov_7.469225"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLO65_14310"
     CDS             complement(<1..186)
                     /locus_tag="PLO65_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29469.1"
                     /translation="MADAAAPADRLAALTRARVLLRDLRGRLRASEAWFIVLAIGLGA
                     AAGLLSVIQGSIARLLQN"
     gene            complement(291..1850)
                     /gene="guaA"
                     /locus_tag="PLO65_14315"
     CDS             complement(291..1850)
                     /gene="guaA"
                     /locus_tag="PLO65_14315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRD29470.1"
                     /translation="MTAPAHQPVLIVDFGSQVTQLIARRVREAGVYCEIHPFDKVEAL
                     VDAFQPAAIILSGGPASVLEPDSPRIGRKLFDLGVPMLCVCYGEQLLCDVLGGTVEGG
                     HAGEFGRAELTIARDHPLFQGLAGVGELETVWMSHGDRVTAIPDGFEIIGTSTGAPFA
                     AIADDARRIYGVQFHPEVVHTVNGAKIYRNFLYNLAGLKGDWTMAAFRQEMIAKIRAQ
                     VGKGRVICGLSGGVDSSVAAVLIHEAIGDQLTCVYVDTGLMRKGESEQVVSLFRNHYN
                     IPLVHVDAADLFLGALEGVSDPETKRKTIGKLFIDVFDAESSKIEGAEFLAQGTLYPD
                     VVESVSARGGPSAVIKSHHNVGGLPDYMKLKLVEPLRELFKDEVRALGVELGLHPDFV
                     GRHPFPGPGLAIRIPGEITREKVAMLQQADAIYLDEIRKAGLYDQIWQAFAVLLPVKT
                     VGVMGDARTYENVLALRAVTSTDGMTADFFEFPWAVLGKTATRIINEVRGVNRVVYDV
                     TSKPPGTIEWE"
     gene            complement(1847..>2687)
                     /locus_tag="PLO65_14320"
     CDS             complement(1847..>2687)
                     /locus_tag="PLO65_14320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRD29471.1"
                     /translation="EMAALAERAPVDLRANSLKTDPDRAAKALQPLHAAPAGILPDAF
                     RIPAPAATDRAGSVETIPQFGKGWFEVQDLGSQIAAACAGEIKGKQVLDFCAGGGGKT
                     LALAAAMGNTGQLYAHDSDARRMADIVPRATRAGVRNLQIRSPVTPDPLKGLEGRMDL
                     VFVDAPCTGAGTWRRHPDSKWRLSPEQLERRMAEQDQVLDQACAYVKPGGRMVYVTCS
                     NFAEENEDRIAAFLARHPGFAVRPAAFEAVAGHATPEGFLRLTPLSAATDGFFVALME
                     RIA"
BASE COUNT          403 a          904 c          965 g          415 t
ORIGIN      
        1 attctgaagc aggcgggcga tggagccctg gatcacgctg agcagaccgg cggccgcgcc
       61 gaggccgatg gccaggacga tgaaccaggc ttcgctggct ctcaggcgac cgcgcaggtc
      121 ccgcagcagc acccgcgcgc gggtcagggc cgcgagacgg tcagccgggg cggcggcgtc
      181 ggccacctct caggaccggc ccgtcgacga ccaccccggg gtggccgcca tgctgtcgac
      241 gatggtcatg tctcacaaac gcctcatgcc atccgggatc accgcccggc ctattcccac
      301 tcgatggtgc cgggcggctt gctggtcaca tcgtagacca cgcggttgac gccgcgcacc
      361 tcgttgatga tccgggtggc cgtcttgccc agcaccgccc acgggaactc gaagaagtcg
      421 gcggtcatgc cgtcggtgct ggtcaccgcg cgcagggcca ggacgttctc gtaggtgcgg
      481 gcgtcgccca tcacaccgac ggtcttgacc ggcagcagca cggcgaaggc ctgccagatc
      541 tggtcgtaca ggccggcctt gcggatctcg tcgaggtaga tggcgtcggc ctgctgcagc
      601 atggcgacct tctcgcgggt gatctcgccg gggatgcgga tggccaggcc gggccccggg
      661 aacgggtggc ggccgacaaa gtcagggtgc aggccgagct cgacgcccag cgcccggact
      721 tcgtccttga acagctcgcg cagcggctcg accagcttca gtttcatgta gtccggcagg
      781 ccgccgacgt tgtggtggct tttgatcacc gccgacgggc cgccccgcgc cgagacgctc
      841 tcgacgacgt ccggatagag ggtgccctgg gcgaggaatt cggcgccctc gatcttcgac
      901 gactccgcgt cgaacacgtc gatgaacagc ttgccgatgg tcttgcgctt ggtttccgga
      961 tcgctgacgc cttcgagcgc gccgaggaaa agatcagcag cgtcaacatg gaccagcgga
     1021 atattgtagt ggttgcggaa cagggagacg acctgctcgc tctcgccctt ccgcatcagg
     1081 ccggtgtcga catagacgca ggtcagctgg tcgccgatgg cctcgtggat cagcaccgcg
     1141 gccaccgagc tgtcgacgcc gccgctgagg ccgcagatca cccggccctt gccgacctgg
     1201 gcgcggatct tcgcgatcat ttcctggcgg aaggccgcca tggtccagtc gcccttcagg
     1261 ccggcgaggt tgtagaggaa gttgcggtag atcttcgcgc cgttgaccgt gtgaacgacc
     1321 tcggggtgga actgcacacc gtagatgcgg cgggcgtcgt cggcgatggc ggcgaacggc
     1381 gcgccggtcg aggttccgat gatctcgaag ccgtcgggga tggcggtgac gcggtcgccg
     1441 tggctcatcc agacggtttc cagctcgccg acgccggcca ggccctggaa cagagggtgg
     1501 tccctggcga tggtcagctc ggccctgccg aactcgccgg cgtggccgcc ctcgaccgtg
     1561 ccgcccagca cgtcgcacag cagctgctcg ccatagcaga cgcagagcat cggcacgccg
     1621 aggtcgaaca gcttcctgcc gatccgcggg ctatcgggct ccagcacgct ggccgggccg
     1681 cccgacagga tgatcgccgc cggctggaag gcgtcgacca gcgcctcgac cttgtcgaac
     1741 ggatggatct cgcaatagac ccccgcctcc cgcacccggc gggcgatcag ctgcgtcacc
     1801 tggctgccga agtcgacgat caggacgggc tggtgggcgg gggcggtcat gcaatgcgtt
     1861 ccataagggc gacgaagaag ccgtcggtgg cggcggaaag gggcgtcagg cgcaggaacc
     1921 cctcgggtgt ggcatgtccg gcgaccgcct cgaaggccgc cggacgcacg gcgaaacccg
     1981 gatggcgcgc caggaaggcc gcgatgcggt cctcgttctc ttccgcgaag ttcgagcagg
     2041 tgacgtagac catgcgacca ccgggcttca cataggcgca ggcctggtcg agcacctggt
     2101 cctgctcggc catccggcgt tcgagctgct cgggcgacag ccgccacttc gagtcggggt
     2161 gccgccgcca ggtgccggcg ccagtacagg gggcgtcgac gaacacaagg tccatcctgc
     2221 cctccagccc cttgagcggg tccggtgtca ccggcgagcg gatctgcagg ttgcgcactc
     2281 cggcgcgggt ggcgcgcggg acgatgtcgg ccatgcgccg ggcgtcgctg tcgtgggcgt
     2341 agagctggcc ggtgttgccc atggccgcgg ccagggccag ggtcttgccc ccgcccccgg
     2401 cgcagaagtc gagcacctgt ttgcccttga tttcgcccgc gcaggcggcg gcgatctggc
     2461 tgcccaggtc ctgcacctcg aaccagccct tgccgaactg cgggatggtc tcgaccgaac
     2521 cggcccggtc ggtggcggcg ggcgcgggaa tgcggaaggc gtcgggcagg ataccggccg
     2581 gcgcggcgtg cagcggctgc agggccttcg cggcgcggtc ggggtcggtc ttgaggctgt
     2641 tggcgcgcag gtccaccggc gcccgctcgg ccagggcggc catctcg
//
LOCUS       DAORNX010000401         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28752_length_2683_cov_4.818113, whole genome shotgun sequence.
ACCESSION   DAORNX010000401 DAORNX010000000
VERSION     DAORNX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28752_length_2683_cov_4.818113"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="PLO65_14325"
     CDS             <1..2079
                     /locus_tag="PLO65_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD29472.1"
                     /translation="AGLSAARALADRLETEAREALIGQGAEAGVVERRLRLRYDGADA
                     ALPIALSDGRAAKAAFEDAHQRLFGFVEPDRTVLIASVEVEATESSPGAPDPGLVPKP
                     SVVSGPIREHPGPGLRQDERRVVHADQLGELSGPALIIRSDTQIAVADGWRASAEPDG
                     LIRLTRATPLTAAAIAADAPDPVTLELFNRRFMGVAEAMGAALERTAHSVNIKERLDF
                     SCALFDADGGLVANAPHMPVHLGSMGASVRATTARHPVLRPGEAFALNNPYFGGTHLP
                     DITVVMPVFMDDAAPPAFYVAARGHHADVGGVQPGSMPPFSTTLDEEGVLLDAVPIMR
                     NGRFLEAETRAALLTGRWPARAPDRNIADLKAQIAACQAGAAAVAAMIEAYGPDAVAR
                     YMTFVQRNAEQAVRRAVTKLHDGEARVPMDGGGEIVVTIRVDSAAGEAVLDFTGSFDQ
                     LASNFNAPSAIVDAAALYVFRSLVDDDIPLNAGCLTPLKILTRHGSMLDPEPPAAVVA
                     GNVETSQHVVDALYAALGVMANSQGTMNNFTFGDEDRQYYETICGGAGATAGRDGTSA
                     VHSHMTNSRLTDPEILERRFPVRVESFAVRPDSGGAGARRGGDGAIRRVRFLAPMEAA
                     LLSSRRSHSPQGICGGGPALPGRQRLITTDGAVRELTGCFSISVRPGDVVEIETPGGG
                     GFGPPRDGDA"
     gene            2102..>2683
                     /locus_tag="PLO65_14330"
     CDS             2102..>2683
                     /locus_tag="PLO65_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29473.1"
                     /translation="MAPLVFALALILQDAPAPEAAGDGPVATAVPRKQDVAPLPIQGG
                     PVSAPTDDYGYVGWCYGAISGYVDLYDRAMPEVTRIERAWPTPSTEDNIKVVYPGQRD
                     EARRNLALFARAMEAAEKASPTPIQMQGAAAIKRGRAVWTGATSVPKAQLAQFWMSWS
                     PPAKCEETAKTLETRSLLFGQALTYNAKGAAPIV"
BASE COUNT          400 a          974 c          938 g          371 t
ORIGIN      
        1 gccgggctgt ccgccgcgcg cgcgctggcg gaccggctgg agacggaggc ccgcgaggcc
       61 ctgatcggcc agggggccga ggccggcgtg gtcgaacgcc gccttcgcct gcgttacgac
      121 ggggcggatg cggccctgcc gatcgccctg tcggacgggc gcgccgcgaa agccgccttc
      181 gaggacgccc accagaggct gttcgggttc gtcgagccgg accggaccgt cctgatcgcc
      241 agcgtggagg tggaggccac ggaatcgtcc cccggagccc cggatccagg cctggtcccg
      301 aagccgtcag ttgtgtccgg accgatccgg gaacacccgg gccccggcct tcgccaggat
      361 gaacggaggg tggtgcatgc cgaccagctc ggtgagttga gcggccccgc cctgatcatc
      421 cgcagcgaca cccagatcgc cgtggccgac ggctggcgcg cctcggccga gcccgacggc
      481 ctgatccgac tgacccgcgc caccccgttg acggccgccg ccatcgccgc cgacgcgccc
      541 gacccggtga ccctggagct gttcaaccgc cgcttcatgg gcgtggccga ggccatgggc
      601 gcggcgctgg agcgcacggc gcattcggtc aacatcaagg aacggctcga cttttcctgc
      661 gccctgttcg acgccgatgg cgggctggtg gccaacgcgc cgcacatgcc ggtgcatctg
      721 ggcagcatgg gcgccagcgt gcgggccacg acggcgcggc atccggtgct gcggccgggc
      781 gaggctttcg ccctcaacaa tccctatttc ggcggcaccc acctgcccga tatcaccgtg
      841 gtgatgccgg tgttcatgga cgacgccgct ccccccgcct tctacgtcgc cgcccggggc
      901 caccatgccg atgtcggcgg ggtgcagccc gggtctatgc cgcccttctc gacgacgctc
      961 gacgaggaag gcgtgctgct cgacgccgtg ccgatcatgc ggaacgggcg ttttctcgag
     1021 gccgagaccc gcgcggccct gctgaccggc cgctggccgg cccgggcgcc ggatcgcaac
     1081 atcgccgacc tcaaggccca gatcgccgcc tgccaggccg gcgcggcggc ggtggcggcg
     1141 atgatcgagg cctacggtcc cgacgccgtc gcccgctaca tgaccttcgt gcagaggaac
     1201 gccgaacagg cggtgcggcg ggcggtcacg aaactccatg acggcgaggc ccgcgtgccg
     1261 atggacggcg gcggcgaaat cgtggtgacc atccgggtcg actcggcggc gggcgaggcc
     1321 gtgctcgact tcaccggctc gttcgaccag ctggcgagca acttcaacgc cccctcggcg
     1381 atcgtcgacg cggccgcgct gtacgtgttc cgcagcctgg tcgacgacga catcccgctg
     1441 aacgccggct gcctgacgcc gctgaagatc ctcacccgcc acggctcgat gctcgatccg
     1501 gaaccgcccg cggcggtggt cgccggcaac gtcgagacca gccagcacgt ggtggacgcc
     1561 ctctatgccg ccctgggggt gatggccaac agccagggca cgatgaacaa cttcaccttc
     1621 ggcgacgagg accggcagta ctacgagacg atctgcggcg gcgcgggagc gacggcgggc
     1681 cgcgacggaa ccagcgctgt gcacagccat atgaccaaca gccggctgac cgatccggaa
     1741 attctcgagc gccggtttcc ggtgcgggtg gagagcttcg ccgtccggcc ggactccggc
     1801 ggcgccggcg cacgacgagg tggcgacggg gccatccgcc gcgtccgctt tctcgcaccg
     1861 atggaggccg ccctgctctc gtcacgccga agccacagtc cgcagggaat ctgcggcggc
     1921 ggaccggcct tgcccggccg gcagcgtttg ataaccaccg acggcgcggt aagggaacta
     1981 accggctgtt tttcgatttc cgtgcggccc ggcgacgttg tcgagatcga gacccccggg
     2041 ggcggcggtt tcggtccgcc cagggacggc gacgcctgac gcaacactga taggaccttg
     2101 aatggccccg cttgtcttcg ccctcgccct gatcctgcag gacgctcccg cgccggaagc
     2161 cgccggcgac ggtccggtcg ccaccgccgt cccgcgcaaa caggacgtcg cgccgttgcc
     2221 catccagggt ggcccggtgt cggctccgac cgacgactat ggttacgtcg gctggtgcta
     2281 cggcgcgatc tcgggctacg tcgacctgta cgaccgggcc atgcccgagg tgacccgcat
     2341 cgagcgcgcc tggccgacgc cgagcaccga ggacaacatc aaggtcgtct atccgggcca
     2401 gcgggacgag gccaggcgca acctggccct gttcgcgcgc gcgatggaag ccgccgaaaa
     2461 ggccagtccc acgccgatcc agatgcaggg cgccgcggcc atcaagcgcg gccgggcggt
     2521 atggaccggc gccaccagcg tgcccaaggc gcagctggcg cagttctgga tgtcctggtc
     2581 gccgccggcc aagtgcgagg aaaccgccaa gactctcgag acgcggtccc tgctgttcgg
     2641 ccaggccctg acctacaatg ccaagggcgc ggcgccgatc gtc
//
LOCUS       DAORNX010000402         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28761_length_2682_cov_6.250856, whole genome shotgun sequence.
ACCESSION   DAORNX010000402 DAORNX010000000
VERSION     DAORNX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28761_length_2682_cov_6.250856"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PLO65_14335"
     CDS             complement(<1..1631)
                     /locus_tag="PLO65_14335"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRD29474.1"
                     /translation="MATEQGGTRHRALKTAGLATIVAFLVALFDPLGLDQAGDRRSAE
                     AADRVAAPFYPSGHGRALVTTVPIDEAALRSVGREWPPQYDFYAEVLDALTADAATRP
                     AAIFLDFTFMSELYSEERRDRFVARVHQVTRAESWTGRPECHTTPTAKLRCIIAHGGT
                     PVIIGKPYPVDRCDLTLTLLLLDRAAVLTPLGWPGMGDAWRSAISRDQYRSQRRGDEE
                     GCPALEGIEAAGGKASFDYGSAVLNAMIGAPSVKVTARGVARYDLTPSAALFAALCLR
                     DRRVSPHCGNPGNPAFEAFLARETTFKVFWGSIPDPAYLAVEERLFPARADVERRERC
                     RRETAGPGRVLAVGLAQLLAGLDDRGAARPVECGYHADLRYDLLTGDTADPAIASDLR
                     RAFVAGRAVIVGAALAQSNDWTVSPTRGRVPGMNLHAMALDNLLELGGRMPRPPPAIL
                     GSPIDEGEAVELILTFLMTLLIVTADERAPAAAAGAPDAGAIRRRRLGLAAMSIGGSL
                     LVFVVASLFLTTVLRWTPVNIVGLWVLSVTVAFVS"
     gene            1793..2431
                     /locus_tag="PLO65_14340"
     CDS             1793..2431
                     /locus_tag="PLO65_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD29475.1"
                     /translation="MMQPGQPKWRRRKDARPAEIVAAALEVFAEKGFAAARLDDIAAR
                     AGVSKGALYLYFETKQDLFAAVVRDAISPNIAVVEATAAAIDLPFAQTSRLLLGRAAD
                     LLATSPVGPVAKMVIAESRNFPDLARVWHDEVVSRILAVISGLIERAQARGEIQPGDP
                     RFHALALIGPLLMGVLWREVMEPVGAAPIDLKALASQHVETALHGLLAEGGR"
     gene            2431..>2682
                     /locus_tag="PLO65_14345"
     CDS             2431..>2682
                     /locus_tag="PLO65_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRD29476.1"
                     /translation="MNPKARIAVVAVVILAAVGAWWTWGRKPVRGAALSGYIEGEALY
                     LSSSTAGPVSRIAVVRGQRVVAGQPLFALDAGPAVALRDQ"
BASE COUNT          409 a          933 c          962 g          378 t
ORIGIN      
        1 aacgagacga acgccacggt gacgctgagg acccagaggc cgacgatgtt gaccggcgtc
       61 cagcgcagaa cggtggtgag aaacaggctg gccaccacaa acaccagcag agatcccccg
      121 atcgacatcg ccgccagacc cagacggcgc cggcggatcg ccccggcgtc cggcgctccg
      181 gcggctgcgg ccggggcccg ttcgtcggcc gtcacgatca ggagggtcat gaggaaggtc
      241 aggatcagtt cgaccgcctc gccctcatcg atcgggctgc cgagaatggc cgggggcggc
      301 cggggcattc ggccgcccag ttccagcagg ttgtccagcg ccatggcgtg caggttcatg
      361 ccagggacac gtccgcgcgt cggcgagacg gtccagtcgt tgctctgcgc cagcgcggcg
      421 ccgacgatca ccgcccggcc ggccacgaag gcccggcgaa ggtcggaggc gattgccggg
      481 tcggcggtgt cgccggttag caggtcgtac cggaggtcgg cgtgatagcc gcactcgacc
      541 gggcgggcgg cgccccggtc gtccagcccg gccagcagct gggccagccc gaccgccagg
      601 acgcgccccg gaccggcggt ttcccgtctg caccgttcgc ggcgctcgac gtcggcgcgg
      661 gccgggaaca gcctttcctc gacagcgaga taggccggat cggggatcga gccccagaac
      721 accttgaagg ttgtctcccg ggcgagaaag gcctcgaagg ccgggttgcc ggggttgcca
      781 cagtgggggg aaacccggcg gtcgcgcagg cagagggcgg cgaacagcgc tgccgagggc
      841 gtcaggtcat agcgcgcgac gccccgcgcc gtgaccttga ccgacggcgc cccgatcatc
      901 gcgttcagaa ccgccgagcc gtagtcgaaa ctggcctttc cgccggcggc ctcgatgccc
      961 tccagcgccg ggcagccctc ttcgtcgccc cgccgctggc tccggtactg atcgcgggaa
     1021 atggccgacc gccaggcgtc gcccatgccg ggccagccca gcggcgtgag aacggccgcc
     1081 cggtcgagca ggagcagcgt cagggtcagg tcgcagcggt cgacggggta gggcttgccg
     1141 atgatcaccg gcgtcccgcc atgggcgatg atgcacctca gcttggcggt gggcgtcgtg
     1201 tggcattcgg gccggccggt ccagctttcg gcgcgtgtga cctggtggac cctggccacg
     1261 aagcggtccc gccgttcctc gctgtagagt tccgacatga aggtgaaatc gaggaagata
     1321 gcggccggtc gggtcgcggc gtcggcggtc agcgcgtcga gcacttcggc gtagaagtcg
     1381 tactgtggag gccattcccg gccgaccgac cggagggcag cctcgtcgat cggcacggta
     1441 gtcacgagcg cgcgcccgtg gccggacgga tagaagggcg ccgccacccg atcggccgcc
     1501 tcggcgctgc ggcggtcacc ggcctgatcc aggccgaggg gatcgaacag ggcgaccaga
     1561 aacgcgacga tcgtggccag gcccgccgtc ttcaacgccc gatgccgcgt tccaccctgt
     1621 tctgtcgcca cgctccaatc cccgataacg acgttatcat cgcattaacc aatggcaacc
     1681 tgcggatgtc cgcgccggtg atgccgaccg gccggcgact ttcggaccgc aacccttgcc
     1741 gcaagcctcc cggtgatata aataactgac cggtcagtta taaagggcag gtatgatgca
     1801 gccgggtcag cccaaatggc gacgtcgcaa ggacgcccgg ccggcggaga tcgtcgccgc
     1861 ggcgctcgag gtgttcgccg agaaggggtt cgcggccgcc cggctcgacg acatcgccgc
     1921 ccgggcgggg gtcagcaagg gcgccctcta cctctacttc gagaccaagc aggacctgtt
     1981 cgccgccgtg gtgcgcgacg ccatcagccc caacatcgcc gtggtcgagg cgaccgccgc
     2041 ggccatcgac ctgccgttcg cccagaccag ccgcctgctg ctgggccgcg cggccgacct
     2101 gctggcgacc tcacccgtcg ggccggtggc caagatggtc atcgccgagt cgcggaactt
     2161 ccccgatctg gcgcgggtct ggcacgacga ggtggtcagc cggatcctgg ccgtcatcag
     2221 cggcctgatc gaacgggccc aggcgcgcgg cgagatccaa cccggcgacc cgcgcttcca
     2281 cgccctggcc ctgatcggtc cgttgctgat gggggtgctg tggcgggaag tgatggagcc
     2341 ggtcggcgcc gcgccgatcg acctcaaggc gctggccagc cagcatgtcg agaccgccct
     2401 gcacggtctt ctggccgaag gaggccgctg atgaacccga aagctcgcat cgccgtcgtc
     2461 gcggtggtca tcctcgccgc cgtcggcgcc tggtggacct ggggtcgcaa gccggtccgg
     2521 ggcgcggccc tgtcgggcta catcgagggg gaagcgctgt acctgtcctc ttccaccgcc
     2581 ggaccggtgt cgcggatcgc cgtggttcgg gggcagaggg tcgtcgcggg ccagccgctg
     2641 ttcgccctcg acgccgggcc ggcggtcgcc ctgcgcgacc ag
//
LOCUS       DAORNX010000403         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28865_length_2673_cov_8.292590, whole genome shotgun sequence.
ACCESSION   DAORNX010000403 DAORNX010000000
VERSION     DAORNX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28865_length_2673_cov_8.292590"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PLO65_14350"
     CDS             complement(<1..1450)
                     /locus_tag="PLO65_14350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD29477.1"
                     /translation="MTPSLTFHGAAGCVTGFCAELRTDTARVLIDCGMFQGSKTLKAL
                     NYEPFPFHAGDIDAVLLTHAHIDHSGLLPKLMLAGFEGPIYATAATRDLCAVMLADAG
                     GIQEGEVRDLNRRNQRRGRPTVEPIFTEQDAKRVMPQFERVKMGEAVEVAPGLTAVYW
                     QAGHILGAASIEVIVAGEDGPMRLFFSGDIGPGGSEFVADPQGPVGVDHLVMESTYGD
                     RDRVITDAVARRKALAQELRDAHDAGGPLLIPAFAVERSQELLADLLAVMDSGEAPGG
                     DVFLDSPLAIEATEVFQRRGWNPDEGRNPFEGVRPSERLRFLEKPWDSDGLDRLKGWH
                     VILAASGMCDAGRIRKHLKRLLWRKETTVLLSGFQAVGTLGRLLAEGARRVTIQGEEI
                     RVKARIRMLDAYSGHADAGGLVSWAQSRGPITGSVFLAHGEPHAVEGLRDRLVAAGFP
                     PDQLIAPRIDESFLLRKARAEAGPAAAARIPPG"
     gene            complement(1447..2334)
                     /gene="prs"
                     /locus_tag="PLO65_14355"
     CDS             complement(1447..2334)
                     /gene="prs"
                     /locus_tag="PLO65_14355"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRD29478.1"
                     /translation="MRRVEVHGFADDADAVRRLAEGLGVAHALVDLRTFPDGETLPTV
                     ATTGADTVILLRSLFPANARLVPLLLAADAYRRTGVKRLTLAAPYLCYLRQDTVFEAG
                     QPLSRDVVCRWLGEAFDRLVTVQAHLHRTTDLAAVLGIRADNIDIGADLAALAVRDGP
                     PLIVGPDSESRPWVEAAARRLGGDWTVFAKQRLGDRRVALTLDSAARIAGRNVLLLDD
                     ICSSGGTLEQATLMLRQAGAARVDIAVAHALFDAAAEARLRRAGADRIVSSDSVPHST
                     NALDLAGVLAAALRDEIPA"
     gene            complement(2331..2639)
                     /locus_tag="PLO65_14360"
     CDS             complement(2331..2639)
                     /locus_tag="PLO65_14360"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29479.1"
                     /translation="MDAIVDAQGPSPLKAAIGPMAHEVLATRAGPVEAIDCLRIANIA
                     RLAGAPTDPGAGVELLRRIGEPVRKGDPLYRVYGTDPSDFGFAVEAAEADSGFRVDAS
                     "
BASE COUNT          398 a          948 c          965 g          362 t
ORIGIN      
        1 ccccgggcgg gatccgcgct gccgcggccg ggccggcctc ggcccgcgcc ttgcgcagca
       61 ggaagctctc gtcgatccgc ggtgcaatca gctggtccgg cgggaagccc gccgcgacca
      121 ggcggtcacg caggccctcg acggcgtgcg gctcgccgtg agcgaggaag accgaccctg
      181 tgatcgggcc gcgggactgg gcccagctga ccaggccgcc ggcgtcggcg tgaccggaat
      241 aggcatccag catccggatg cgcgccttca cgcgaatttc ctcgccctgg atcgtcaccc
      301 ggcgggcgcc ctcggccagc agccggccca gcgtcccgac ggcctggaaa ccggacagca
      361 ggaccgtggt ctccttgcgc cacagcaacc gcttcaggtg cttgcggatg cggccggcgt
      421 cacacatgcc gctggcggcg aggatcacat gccagccctt gagccggtcc aggccgtcgc
      481 tgtcccaggg cttttcgagg aagcgcagcc gctccgatgg acggacgccc tcgaacggat
      541 tgcgcccttc gtccggattc cagccgcgcc gctggaacac ctcggtcgcc tcgatggcca
      601 gcggggaatc gagaaagacg tcgccgcccg gcgcttcgcc gctgtccatt accgccagca
      661 ggtcggccag cagttcctgg ctgcgctcga ccgcgaaggc ggggatcagc agaggcccgc
      721 cggcgtcgtg ggcgtcgcgc agctcctgcg ccagcgcttt ccggcgggcc acggcgtcgg
      781 tgatgacccg gtcgcggtca ccgtaggtgg actccatgac caggtggtcg acgccgaccg
      841 gcccctgggg atcggcgacg aactcgctgc cgccgggccc gatgtcgccc gagaagaaca
      901 ggcgcatcgg accgtcctcg ccggcaacga tcacctcgat cgaggccgcg cccagaatgt
      961 gacccgcctg ccagtagacc gccgtcagcc cgggcgcgac ctcgacggcc tcacccatct
     1021 tgacccgctc gaactgcggc atgacgcgct tggcgtcctg ttcggtgaag atcggctcga
     1081 ccgttggccg cccacggcgc tggttgcggc ggttcagatc gcggacctcg ccctcctgga
     1141 tgccgccggc gtcggccagc atcaccgcgc acaggtcccg cgtcgcggcg gtcgcgtaga
     1201 tcggcccttc gaacccggcc agcatcagct tgggcagaag gccggaatgg tcgatgtgcg
     1261 catgggtcag cagcaccgcg tcgatgtcgc cggcgtggaa ggggaagggc tcgtagttga
     1321 gcgccttcag cgtcttggac ccctggaaca tgccgcaatc gatcagcacc cgggccgtgt
     1381 cggtgcgcag ctcggcgcag aagccggtga cgcagccggc cgcgccgtga aaggtcaggg
     1441 agggggtcat gccgggatct cgtctcgcag ggcggcggcg aggacgccgg ccaggtcaag
     1501 ggcgttggtg gaatggggga cagagtcgct ggagacgatc cggtcggcgc ccgctcggcg
     1561 cagtcgggcc tcggcggcgg cgtcgaacag ggcatgggcc acggcgatgt cgacccgcgc
     1621 cgccccggcc tggcgcagca tcagggtcgc ctgttcgagt gtgccgccgc tggaacagat
     1681 gtcatccagc aagagcacat tccgtccggc aattctggcg gcggagtcga gtgtcagcgc
     1741 gacccgccgg tcgccgagcc gttgcttggc gaagactgtc cagtcgccgc cgaggcgacg
     1801 ggccgcggcc tcgacccatg gccgcgactc gctgtccggc ccgacaatca ggggcggacc
     1861 gtccctgacc gccagggcgg cgaggtccgc gccgatgtcg atgttgtcgg cccgtatgcc
     1921 cagcaccgcg gccagatcgg tcgtgcgatg gagatgagcc tggactgtca cgaggcggtc
     1981 gaaggcctcg cccagccaac ggcagacgac gtcgcggctg agcggctggc cggcctcgaa
     2041 gaccgtgtcc tggcgcagat aacagaggta gggcgcggcc agcgtcagcc gcttcacgcc
     2101 ggtccgccga taggcgtcgg cggccagcag cagcggaacc agccgggcgt tggccgggaa
     2161 gagcgaacga agcaggatga ccgtgtcggc gccggtcgtc gcaacggtcg gcagggtctc
     2221 cccgtcggga aaggtccgca gatcgacaag ggcatgggcc acgccgagcc cctcggcgag
     2281 gcgccggacc gcatcggcgt cgtcggcgaa cccgtgcacc tcgacccgcc tcatgacgcg
     2341 tcgacccgaa agccggagtc cgcctcggcc gcctcgacgg caaacccgaa atccgacggg
     2401 tccgtgccgt agacgcggta gagcgggtcg cccttgcgga ccggctcgcc gatccgtcgc
     2461 aacagctcga cgccggcgcc ggggtcggtg ggcgctcccg cgagccgcgc gatgttggcg
     2521 atgcgcagac agtcgatcgc ctcgaccggc ccggcccgcg tggcgagcac ttcatgcgcc
     2581 atcggcccga tcgcggcctt cagcggggac ggcccctggg cgtcgacgat ggcgtccatg
     2641 cgggccctcg cggcgccgga ttcgagcaac tcg
//
LOCUS       DAORNX010000404         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28942_length_2667_cov_7.882083, whole genome shotgun sequence.
ACCESSION   DAORNX010000404 DAORNX010000000
VERSION     DAORNX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28942_length_2667_cov_7.882083"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..>2667
                     /locus_tag="PLO65_14365"
     CDS             299..>2667
                     /locus_tag="PLO65_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD29480.1"
                     /translation="MVIVAGVVCLCASLTAFRLFSRMNGARGLVRGAWLLLTALVAGS
                     GVWATHFLAMLAYDPGFKTGYSPTGTMLSLMIVVFFMAAGFFAATASRDIANKLAGGL
                     LIGLGVGAMHFTGMSSFVTQGTIVWEAATIGASVVLGVIGATLALLAAGEARSLPRQA
                     LAGVILTLAVCALHFTAMGAITIMPDATVVVPDQLFSGGVMTLAVASITGLIILGGLG
                     AVLIESATSRSALERIRTLANAAYEGIVVVQNGVIKDANAAFCELAGAELDVLLGTTL
                     GQAVLTFDDALGVRTDAGRREGHMHPVNGGHPVPVEVFARVMDDGARAETRGLMVLAI
                     RDLRERRSAEEKIRYLAEHDGLTGLPNRNALQARLAAALDRVEASGEALALICVDLDH
                     FKEANDLHGHVAGDAVLVEAARRLQDSVTAPSFAARLGGDEFVVVQITDQDQPSAAAE
                     LAGQLLEALKGGMTFEGQELAMAASLGVSLFPDDGRTGEALLANADMALYRAKENGRG
                     TYRFFKREMDETIRERRNLARELRQAILDEDLVVHYQPQARASDGEICGFEALVRWRH
                     PVRGMIPPLDFIPLAEETGLIIPLGEWVLRKACAEAAAWDKPLSIAVNLSPLQLNQPN
                     LPQTVHEILIESGLSPMRLELEVTESALFKDYQRALDNLRRLKALGVRIAMDDFGTGF
                     SSLSTLQSFPFDKIKVDKSFVENIHRDPRATVIVKAVLGLGRSLDIPVVAEGVETDEQ
                     LQFLRGEDCAEVQGYAIGRPAPADTLGAWTNDLPAVLIAAALGDAPTRAR"
BASE COUNT          384 a          964 c          921 g          398 t
ORIGIN      
        1 tcgtcgccgc ctataaccgc aaggcgaccg gggagaagtt cctcatcaat cccgacctgg
       61 gctaggcgcg gtcgaccctc ggcgccgggg acagctggcg cgaagacggc cctcgaccat
      121 cgaccgtcga cccacgcgga gcggccggcg acgggccgcc gcacggtgtc gcacccgtcc
      181 cggattgaaa cgctttcttt gcaactcgcc cggatactcc cctcagctgg gatcctcaga
      241 acggggtggg gcggtgttca aggtcttctc gtgtctcgtc aacgagcacg actaccggat
      301 ggtgatcgtc gccggggtgg tctgcctctg tgcgagcctg acggcgttcc ggctgttctc
      361 gcgcatgaac ggggcgcggg ggctggtgcg cggcgcctgg ctgctgctga ccgccctggt
      421 cgccggctcg ggagtctggg cgacccactt cctcgccatg ctggcctacg atcccgggtt
      481 caagaccggc tattcgccga ccggcaccat gctgtccctg atgatcgtgg tgttcttcat
      541 ggccgccggc ttcttcgccg ccaccgccag ccgcgacatc gccaacaagc tggccggggg
      601 cctgctgatc ggcctgggcg tcggagccat gcacttcacc ggcatgtcct cgttcgtcac
      661 ccagggaacc attgtctggg aggcggcgac catcggcgcc tcggtggtgc tgggcgtgat
      721 cggcgcgacc ctggccctgc tggccgccgg cgaggcccgc tcgttgccgc gccaggccct
      781 ggccggggtg atcctgacgc tggcggtgtg cgccctgcac ttcaccgcga tgggcgcgat
      841 caccatcatg ccggacgcca ccgtggtggt gcccgaccaa ctgttctccg gcggggtgat
      901 gaccctggcc gtggcctcga tcaccggcct gatcatcctc ggcggcctgg gggcggtgct
      961 gatcgagtcg gccaccagcc gctcggcgct ggaacgcatc cgcaccctgg ccaacgccgc
     1021 ctacgagggc atcgtggtgg tccagaacgg cgtcatcaag gacgccaacg ccgccttctg
     1081 cgaactggcc ggggccgagc tggacgtact gctgggcacg accctgggac aggccgtgct
     1141 gacctttgac gacgccctgg gcgtccgcac cgacgccgga cgccgcgagg gccacatgca
     1201 tccggtcaac ggcggtcatc ccgtgccggt cgaggtcttc gcccgggtga tggatgacgg
     1261 cgcccgcgcc gaaacccgcg gcctgatggt gctggccatc cgcgacctgc gcgagcgccg
     1321 ctcggccgag gagaagatcc gctatctggc cgagcacgac ggcctgaccg gactgcccaa
     1381 ccgcaacgcc ctgcaggcgc ggctggcggc ggccctcgac cgggtcgagg cgtcgggcga
     1441 ggccctggcc ctgatctgcg tggacctcga ccacttcaag gaagccaacg acctgcacgg
     1501 ccacgtcgcc ggcgacgcgg tgctggtcga ggccgcccgg cggctgcagg acagcgtcac
     1561 cgcgccgtcg ttcgccgcgc gactcggcgg cgacgagttc gtcgtggtgc agatcaccga
     1621 ccaggaccag ccgagcgccg ccgccgaact ggccggccag ctgctcgagg cgctcaaggg
     1681 cggcatgacc ttcgagggcc aggagctggc catggccgcc agcctcggcg tgtcgctgtt
     1741 ccccgacgac ggccgcaccg gcgaggccct gctggccaat gccgacatgg ccctctaccg
     1801 cgccaaggag aacggccgcg ggacctaccg cttcttcaag cgcgagatgg acgagacgat
     1861 ccgtgagcgg cgcaacctcg cgcgcgagct gcgccaggcg atcctcgacg aggacctggt
     1921 ggtgcactac cagccgcagg cgcgcgccag cgacggcgag atctgcggct tcgaggccct
     1981 ggtccgctgg cgccacccgg tgcgcggcat gatcccgccg ctggacttca ttccgctggc
     2041 cgaggagacc ggcctgatca tcccgctcgg cgaatgggtg ctgcgcaagg cctgcgccga
     2101 ggcggccgcc tgggacaagc ccctgagcat cgccgtcaac ctctcgccgt tgcagctgaa
     2161 ccagcccaac ctgccgcaga cggtgcacga gatcctgatc gagtccgggc tgtcgccgat
     2221 gcggcttgag ctggaggtca ccgagagcgc cctgttcaag gactaccagc gggcgctcga
     2281 caacctgcgc cggctcaagg ccctgggcgt gcggatcgcc atggacgact tcggcaccgg
     2341 cttctcgtcg ctgtcgacgc tgcagtcctt cccgttcgac aagatcaagg tcgacaagag
     2401 cttcgtcgag aacatccacc gcgacccgcg cgccacggtg atcgtcaagg ccgtgctggg
     2461 cctgggccgc agcctggaca tcccggtggt cgccgagggg gtcgaaaccg acgagcagct
     2521 gcagttcctg cgcggcgagg actgcgccga ggtgcagggc tacgccatcg gccgcccggc
     2581 cccggccgac accctgggag cctggaccaa cgacctgccg gcggtgctga tcgccgcggc
     2641 cctgggcgac gcgccgaccc gcgcccg
//
LOCUS       DAORNX010000405         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28976_length_2665_cov_7.036398, whole genome shotgun sequence.
ACCESSION   DAORNX010000405 DAORNX010000000
VERSION     DAORNX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28976_length_2665_cov_7.036398"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLO65_14370"
     CDS             complement(<1..487)
                     /locus_tag="PLO65_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD29481.1"
                     /translation="MRDYTKFYIDGQWVQPTGVKTLDVINPANEEVCGKISLGTADHV
                     DQAARAARKAFETFSQTTIEERIDLLERIVVEYQKRYGDMADAITEEMGAPASLAQRA
                     QAAMGLAHTQTAIGVLKSFRFVEDRGPTRLVKEPIGVCGMITPWNWPVNQIACKVAPA
                     LA"
     gene            complement(571..1518)
                     /locus_tag="PLO65_14375"
     CDS             complement(571..1518)
                     /locus_tag="PLO65_14375"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD29482.1"
                     /translation="MAAWMTPADFSRIAVGIDRPEDVVVGKDGRVFASDHQCAVAEIH
                     PDGSFTRMGPKGGAPNGINMDLQGRVLIANFGIYDREEGPLQRFDPATGKHETLVAEV
                     GGRRLTSANYPVLDREGNIWCANSTHADTWPQALDGRTDGFIFVLRPDGSTSIEAEGL
                     KFPNGLALSADDRWLYCAQTSGADVLRFEILPGGKLGAGERYGPQLGRLMAPGDAEAQ
                     AAAEHHPGDLGYTDGVGMDANGNLWVCLPAANKVVAIRPSGVVETVVHDPSGEIVNHP
                     TNVTWGGPDLKDLYIGSIRAGYVLKARSPVAGQPHVHQL"
     gene            complement(1546..2019)
                     /locus_tag="PLO65_14380"
     CDS             complement(1546..2019)
                     /locus_tag="PLO65_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD29483.1"
                     /translation="MSRWKRFLEPRVRPLTRALMRQARGLTLGVRGLVTDGEGRILLV
                     EHTYIHGWYLPGGGVEKRETVEDALARELVEEAGVRLTAPARLMSVHSNERLHPGDHV
                     LIYHCPTWEPCPPTSRGEIRRRDWFAPDALPEGVSAGTRRRIAEMLDGGRPDLFW"
     gene            2189..>2665
                     /locus_tag="PLO65_14385"
     CDS             2189..>2665
                     /locus_tag="PLO65_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29484.1"
                     /translation="MGGAAVITGAARRIGRVLAVQAARSGFDVAVHHRDSPDEAEETA
                     ALVRAEGRRACVLAGDLTVADAADLIARAETALGPVTLLVNNASLFLEDRLETFDAGV
                     LDAHMATNVRAPAVLTQAMAARLAGDRTGLVVNILDQRVWRPNPQFFGYSLSKAALW"
BASE COUNT          378 a          935 c          923 g          429 t
ORIGIN      
        1 cggccagcgc cggggcgacc ttgcaggcga tctggttcac cggccagttc cagggggtga
       61 tcatgccgca gacgccgatc ggctccttga ccagacgggt cgggccgcgg tcctcgacga
      121 acctgaagct cttcagcacg ccgatggcgg tctgggtgtg ggccagaccc atggccgcct
      181 gggcgcgttg cgccagcgag gcgggggcgc ccatttcctc ggtgatggcg tcggccatgt
      241 cgccgtagcg cttctggtac tcgaccacga tgcgctcgag caggtcgatg cgctcctcga
      301 tggtggtctg ggagaaggtc tcgaacgcct tgcgggcggc cctggcggcc tggtcgacgt
      361 ggtcggccgt gcccagggaa atcttgccgc agacctcttc attggccggg ttgatgacgt
      421 cgagcgtctt gacgcccgtg ggctggaccc attggccgtc gatgtagaac ttcgtgtagt
      481 cgcgcatcac cgtcatctcc ctgcgggccg ccgcacggcc tcttcaaacg ctagtttagt
      541 taccgccgat cacctcggaa aggggcgaca tcaaagttga tgcacgtgcg gctggccggc
      601 gaccgggctg cgggccttga gcacgtagcc ggcgcgaatc gagccgatgt agaggtcctt
      661 caggtccggg ccgccccagg tgacgttggt cgggtggttg acgatctcgc cggacgggtc
      721 gtggaccacc gtctcgacca cgccggacgg gcggatggcg accaccttgt tggccgcggg
      781 caggcagacc cagagattgc cattggcgtc catgccgacg ccgtcggtgt agccgagatc
      841 gcccggatga tgctcggcgg cggcctgggc ctcggcgtcg ccgggggcca tcagccgccc
      901 gagttgcggg ccgtagcgct cgcccgcgcc gagcttgccg ccgggcagga tctcgaaccg
      961 cagcacatcc gcgccgctgg tctgggcgca gtagagccag cggtcgtccg ccgacagggc
     1021 caggccgttg ggaaacttca gcccctcggc ctcgatgctg gtcgagccgt cgggccgcag
     1081 cacgaagatg aaaccgtccg tgcggccgtc gagcgcctgg ggccaggtgt cggcgtgggt
     1141 cgagttggcg caccagatgt tcccctcgcg gtcgagcacc gggtagttgg cgctggtcag
     1201 ccgccgcccc ccgacctcgg ccaccagcgt ctcgtgcttg ccggtggccg gatcgaaccg
     1261 ctgcagcggc ccctcttccc tgtcgtagat gccgaaattg gcgatcagca cccggccctg
     1321 caggtccatg ttgatgccgt tcggcgcgcc gcccttcgga cccatgcggg tgaaggagcc
     1381 gtccggatgg atctcggcca ccgcgcactg atgatcggag gcgaacaccc gcccgtcctt
     1441 gcccaccacc acgtcttccg gccggtcgat gccgacggcg atcctgctga agtcagcggg
     1501 ggtcatccag gccgccatgc gcgcctccct gttgtcctgt ccgtcctacc agaacaggtc
     1561 gggccgcccg ccgtccagca tctcggcgat ccgccggcgc gtgccggccg agactccctc
     1621 gggcagggcg tcgggcgcga accagtcccg ccggcggatc tcgccgcgcg acgtgggcgg
     1681 acagggctcc caggtggggc agtgatagat cagcacgtga tcgccggggt ggaggcgctc
     1741 gttggaatgg acggacatca gccgggccgg ggcggtcagc ctgacgccgg cctcctcgac
     1801 cagttcgcgc gccagggcgt cctcgaccgt ttcccgcttc tcgacgccgc cccccggcag
     1861 ataccagccg tggatatagg tgtgctccac cagcaggatc cggccctcac cgtcggtgac
     1921 cagtccgcgg acgccgagcg tcaggccacg cgcctgacgc atcagcgccc gggtcaatgg
     1981 ccggaccctc ggctccagaa aacgcttcca ccgcgacatg atcgacaacc tagaacgccc
     2041 gcttgcccgg cgcgagagcc ggggctaaag ccgttgcgtt cattcaaccg gagccgcgac
     2101 atgctcgcca tcggcgtcat cgccctgttc atcgtcatcc tggccggcat caacgtcttc
     2161 gaattcggcc ggatcgactg atcccgcctt gggcggcgcg gcggtcataa ccggggcggc
     2221 gagacgcatc gggcgggtgc tggccgtcca ggccgcccgg tcgggcttcg atgtcgccgt
     2281 ccaccaccgc gactctccgg acgaggcgga agagacagcc gcgctcgtcc gcgccgaagg
     2341 gcggcgggcc tgcgtcctgg ccggcgatct gaccgtggcc gacgccgccg acctgatcgc
     2401 ccgggcggag accgcgctcg gcccggtgac cctgctggtc aacaacgcct cgctgttcct
     2461 cgaagaccgg ctcgagacct tcgacgccgg ggtgctcgac gcccatatgg cgaccaatgt
     2521 gcgcgccccg gccgtgctga cccaggcgat ggccgcacgg ctggcggggg accggaccgg
     2581 cctggtggtc aatatcctcg accagcgggt atggcggccg aacccgcagt tcttcggcta
     2641 cagtctgagc aaggccgccc tctgg
//
LOCUS       DAORNX010000406         2664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_28991_length_2664_cov_4.320046, whole genome shotgun sequence.
ACCESSION   DAORNX010000406 DAORNX010000000
VERSION     DAORNX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28991_length_2664_cov_4.320046"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="PLO65_14390"
     CDS             complement(<1..1476)
                     /locus_tag="PLO65_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRD29485.1"
                     /translation="MDRRKLGDILLSRGLVSEYDLAAALALQGQTGELLGLLLVRLGA
                     VAEADLLAALSDQLGLPVQGADEGPDPGQVAALLTELRTPPSWWVDRKAAAWRPDPGS
                     DKVICAAAHPLDPALRDRLAHLGEAVQYRLAPRALAAALLGDLVPDMGLAPASGAIAT
                     DDVARIREMAQEAPVIDFVNGVFSEALSRRASDLHLEPYENQFVVRMRIDGLLHTVRQ
                     APRARFDAVCSRIKLLSAMDIGERRLPQDGRQSIRVAGRELDLRVSTLPSAWGESLVL
                     RLLGKSDRLPELVELGLAPDQATALTALVERPNGIVLLTGPTGSGKTTTIYRLLTHLN
                     NGVRKIITVEDPIEFDLPGVVQMQARPDIGLTFAAGLRAMLRQDPDVIMVGEIRDPQT
                     ARIAVQAALTGHLVIATAHTNSAMAGLTRLIDLGVEDYLLADVVRGLVGQRLARSLCA
                     CATPASADQTRRYEMARPAAPDDEPGPPSAWREPRGCERCGG"
     gene            complement(1526..>2664)
                     /locus_tag="PLO65_14395"
     CDS             complement(1526..>2664)
                     /locus_tag="PLO65_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD29486.1"
                     /translation="TVGRARDSGGQVGRASLALMTSATGFMGGLKAALAQNAQAGFVA
                     EFEAIQADMRFHQLAFVRRQARSRGMFVTLSAVAGAAVVLVGYDLLAVEPAVLITVVL
                     VFTRMSGPVLTLYQAAQQFFFGLPAFEAVRALEADLEEVQVPPATSARPPPEGPLTLT
                     AVRFLHPGGGGIAEASLTLPPGEFLGLTGASGAGKTTLLDLMVGLLPPQAGEITVGGA
                     PLDDAGLAGWRDAVAYVAQDPFLFHDTVRRNLTWGDDRVDDAVLMRVLRDVGAEAMVS
                     RLEQGLDTVVGERGARLSGGERQRLAIARALLRRPRLLVLDEATNAIDLPGEADLLAR
                     LAALDPRPAMVMIAHRPESLAHCDRVVVIEAGRIVEPTAPTTAG"
BASE COUNT          396 a          966 c          963 g          339 t
ORIGIN      
        1 cccgccgcaa cgctcgcacc cccggggctc ccgccaggcc gacggcggcc cgggctcgtc
       61 gtcgggggcg gccggccggg ccatttcgta gcgacgcgtc tggtcggccg aggccggcgt
      121 ggcgcaggcg cagaggctgc gcgccagccg ctgaccgacc aggccccgca ccacgtcggc
      181 cagcaggtag tcctccacgc ccagatcgat caggcgcgtc aggccggcca tggccgagtt
      241 ggtgtgtgcg gtggcgatga ccagatgccc ggtcagagcg gcctgcaccg cgatcctcgc
      301 cgtctgggga tcgcggattt cgccgaccat gatcacgtcg ggatcctgcc gcagcatggc
      361 gcgaagaccg gcggcgaagg tcaggccgat gtcgggccgg gcctgcatct gcaccacgcc
      421 gggcaggtcg aactcgatcg ggtcctcgac cgtgatgatc ttgcggactc cgttgttcag
      481 atgagtcagc aggcggtaga tggtcgtggt cttgccggac cctgtcggcc cggtcagcag
      541 cacgatgcca ttgggacgct cgaccagcgc ggtcagcgcc gtggcctggt cgggggccag
      601 gcccagctcg accagttcgg gcaggcgatc ggattttccc agcagacgga gcaccaggga
      661 ctcgccccag gcagacggca gggtcgagac gcgcaggtcg agttcgcggc ccgccacccg
      721 gatcgactgc cggccatcct ggggaagccg gcgctcgccg atgtccatgg ccgagagcag
      781 cttgatccgc gaacagaccg cgtcgaagcg cgccctcggg gcctggcgga ccgtatgcaa
      841 caggccgtcg atgcgcatcc gaacgacgaa ctggttctca tagggctcaa ggtgcaggtc
      901 cgaggcccgg cggctcaagg cctccgagaa gacgccgttg acgaagtcga tgaccggcgc
      961 ctcctgcgcc atctcacgga tgcgcgccac gtcgtcggtc gcgatcgcgc ccgatgcagg
     1021 ggcgagcccc atgtcgggga ccagatcacc gagcagggcc gccgccagcg ccctcggcgc
     1081 gaggcggtat tgcaccgcct cccccagatg ggccagccgg tcgcgcaacg ccggatcgag
     1141 cggatgggcg gcggcgcaga tgaccttgtc ggacccgggg tccggccgcc aggcggccgc
     1201 cttgcggtcg acccaccacg acggcggcgt gcgcagttcg gtcagcaggg cggcaacctg
     1261 gcccgggtcg ggcccctcgt cggcgccctg aaccggcagg cccagctggt cggagagggc
     1321 cgcgagcaga tcggcctccg ccacggcgcc gagacggacc agcaggaggc cgagcagctc
     1381 gcccgtctgc ccctgcagcg ccagggccgc ggcgaggtcg tactcggaga ccagcccccg
     1441 agacagcagt atgtcgccca gtttccgcct gtccatgcgt gactttagag cgcgtttgcc
     1501 ggtcgcggaa aacccccgtc gccgctcagc cggcggtcgt cggggccgtg ggttcgacga
     1561 tgcgcccggc ctcgatcacg acgacgcggt cgcaatgggc caggctctcc ggccggtggg
     1621 cgatcatcac catggccgga cgggggtcga gggcggcgag ccgcgccagg agatctgctt
     1681 cgcccggcag gtcgatcgcg ttcgtggcct cgtccagcac cagcagccgg ggccggcgca
     1741 gcagcgctcg cgcgatggcc aggcgttgcc tttcgccccc ggacaggcgc gcgccgcgct
     1801 cgccgaccac ggtgtcgagg ccctgttcca gccgtgagac catcgcctcc gcgcccacgt
     1861 cgcgcagcac gcgcatcagc acggcgtcat cgacccggtc gtctccccag gtcaggttgc
     1921 gccgcaccgt gtcgtgaaac aggaagggat cctgggccac ataggccacc gcgtcccgcc
     1981 agccggccag gcccgcatcg tcgagcgggg cgccgcccac cgtgatctca ccggcctgag
     2041 gcggcaggag gccgaccatc aggtccagca gggtggtctt gccggccccc gaggcgcccg
     2101 tcagcccgag gaactcgccc ggcggcaagg tcaggctggc ctcggcgatg cccccgccgc
     2161 cggggtgaag gaagcgcacc gccgtcaacg tcaacgggcc ttcaggcggt ggccgcgcgg
     2221 acgtggcggg cgggacctgg acctcttcca ggtccgcctc cagcgccctg acggcctcga
     2281 aggcgggcag gccgaagaag aactgctggg ccgcctggta gagcgtgagc accggcccgc
     2341 tcatgcgggt gaagacgagc acgacggtga tcagcaccgc cggttcgacg gccagcaggt
     2401 cgtagccgac cagcaccacg gccgcgcccg cgaccgccga gagggtgacg aacatgcccc
     2461 ggctgcgggc ctgacggcgg acaaaggcca gctggtgaaa gcgcatgtcc gcctggatgg
     2521 cctcgaactc ggccacgaag ccggcctggg cgttctgggc gagcgcggcc ttgagcccgc
     2581 ccatgaagcc cgtggcgctg gtcatcaggg ccaggctggc ccgccccacc tgcccgccgc
     2641 tgtcgcgcgc ccggccgacg gtca
//
LOCUS       DAORNX010000407         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29067_length_2659_cov_7.284178, whole genome shotgun sequence.
ACCESSION   DAORNX010000407 DAORNX010000000
VERSION     DAORNX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29067_length_2659_cov_7.284178"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLO65_14400"
     CDS             <1..1223
                     /locus_tag="PLO65_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HRD29487.1"
                     /translation="FALDATPAQMMRLRSPTGAESGLATSLSGRARAAGLAGDWNGPA
                     AAIVEGRVYPALDRQIAALKALRPTATADAGIWKIPDGEAFYAGALAFQTTTTKSPDE
                     VHRLGLEQVADLSARLDVLLKAQGLTTGPVGERLAALSRRPDQLYPNTDAGRAELIAA
                     LNVQTEDMRARLPRVFATLPKSPVEIVRVPPDIQDGAPNGYATAPTLDGSRPGRFYIN
                     LKDTAEWPKFALPTLTYHEALPGHQWEGAIALEAKDSSILRRAGLGFAAYGEGWALYA
                     EQLAGELGAYDNNPLGEIGFLQSMQFRAVRMVVDTGMHAKRWSREKATDYMVETTGMP
                     RPRVQREIDRYCIWPGQACSYKIGHTEWVRLREEARARAGARFDIKVFHDVLRQGGMP
                     LVVLASVVEGLFPA"
     gene            1448..2479
                     /locus_tag="PLO65_14405"
     CDS             1448..2479
                     /locus_tag="PLO65_14405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD29488.1"
                     /translation="MTAEPILEPDLPIVDPHHHLWDRPSLPPRREGAHPFEDVIQLTP
                     RYLLDELRADAGSGHNVTATVYVQCGAFYRADGERAMAPVGEVEFVNGVAAMSASGIY
                     GPFRACAGIVGHADLMLGDGAAAVLEAGIRAGGGRFKGIRHVNASDPDPNVLGPMSHT
                     PGGLLGETAFRAGFARLAPLGLSFDAWMLEPQLPELIALTHAFPDTDIILDHVGTPLG
                     IGAYAGRRDERFGDWKASMTELAKSPKVNVKLGGLAMPFPGFPGLDGQARPSSQALAD
                     LWRPYVETTIELFGVDRCMFESNFPVDLWGCDFATLWNAFKRIAAGASDVEKASLFSG
                     TARRVYRLA"
     gene            complement(2464..>2659)
                     /locus_tag="PLO65_14410"
     CDS             complement(2464..>2659)
                     /locus_tag="PLO65_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRD29489.1"
                     /translation="TAGSGDVLAGFIGGLIAQGMDSFEAACAGAWIHAAAARRFGPGL
                     IAEDLPGLAPAVLADLYASR"
BASE COUNT          403 a          943 c          932 g          381 t
ORIGIN      
        1 atttcgccct cgacgcgacc ccggcccaga tgatgcgact gaggtcgccg acgggggccg
       61 aaagcgggct ggccacgtcg ttgtcggggc gggcccgcgc cgctggtctg gcgggcgact
      121 ggaacggccc cgccgccgcg atcgtcgagg gccgggtcta tccggccctt gaccggcaga
      181 tcgccgccct caaggccctg cggcccacgg cgacggccga cgcgggcatc tggaagattc
      241 ccgacggcga ggccttctac gccggggccc tggccttcca gaccaccacg acaaagtcgc
      301 ccgacgaggt ccaccgtctg ggcctcgagc aggtggccga tctgtcggcc cggctggacg
      361 tcctgctgaa ggcccagggg ctgacgaccg gcccggtcgg ggaacggctg gcggctctgt
      421 ccaggcggcc cgaccagctc tatcccaata ccgacgcagg ccgcgccgag ctgatcgcgg
      481 ccctcaatgt ccagaccgag gacatgcgcg ctcgcctgcc ccgcgtcttc gccaccctgc
      541 cgaaatcgcc ggtggaaatc gttcgcgtcc cgcccgacat ccaggacggc gcgcccaacg
      601 gctacgccac ggcgccgacg ctcgacggat cgaggccggg ccggttctac atcaacctga
      661 aggacacggc cgaatggccg aagttcgccc tgccgaccct gacctaccac gaggctctgc
      721 ccggccatca gtgggaggga gcgatcgcgc tggaggccaa ggattcgtcg atcctccgcc
      781 gggccggcct gggcttcgcg gcctatggcg aaggctgggc gctctacgcc gaacagctgg
      841 ccggcgaact gggcgcctac gacaacaatc cgctcggcga gatcggcttc ctgcagtcga
      901 tgcagttccg cgccgtgcgc atggtggtcg acaccggcat gcatgcgaag cgctggtcgc
      961 gcgaaaaggc caccgactac atggtcgaga ccaccggcat gccgcggccg cgcgtgcagc
     1021 gcgagatcga tcgctactgc atctggcccg gccaggcctg cagctacaag atcggccaca
     1081 ccgaatgggt gcggctgcgc gaggaagcca gggcccgcgc cggggcgcgg ttcgacatca
     1141 aggtgttcca cgacgtcctg cgccagggcg gcatgccgct ggtggtgctg gcgtcggtgg
     1201 tcgaggggtt gttcccggcc tgaccttgcc gtgaaacgga gctcaggctg ctgacacgaa
     1261 cgctgtcatc ccggccgaag cgtagcggag agccgggacc cggattcggg cgagactcga
     1321 tgtgggtccc ggatcgcctg cgccgtcagg gatgaccggc cttgatggcc tgcctcgccg
     1381 ccgccggacc ttgcgaggcg tctggcgacg ggggaccatc gaccagaaac agagaaagcg
     1441 cagctgaatg accgccgaac cgatccttga gcccgacctg ccgatcgtcg atccgcacca
     1501 tcacctgtgg gaccggcctt ccctgccgcc tcgacgcgag ggcgcccatc cgttcgagga
     1561 cgtcatccag ctgacgcccc gctacctgct ggacgagctg cgggccgacg ccggcagcgg
     1621 ccacaacgtc accgccacgg tctacgtcca gtgcggcgcc ttctaccggg ccgatggcga
     1681 gcgggccatg gcgccggtcg gcgaggtcga gttcgtcaat ggcgtggcgg cgatgagcgc
     1741 cagcgggatc tacggcccgt tccgcgcctg cgccggcatc gtcggccatg cggatctgat
     1801 gttgggcgat ggcgcggcgg cggtgctgga ggccgggatt cgcgccggcg gcgggcggtt
     1861 caagggcatc cggcatgtca acgcctctga ccccgatccc aatgtcctcg gcccgatgag
     1921 ccacacgcca ggcggcctgc tgggcgagac cgccttccgc gccggcttcg cccgactggc
     1981 gccgctgggc ctgtcgttcg acgcctggat gctcgagccg cagctgcccg agttgatcgc
     2041 cctaactcac gccttcccgg acaccgacat catcctggac catgtcggca cgccgctggg
     2101 catcggcgcc tatgcgggcc gacgggacga gcggttcgga gactggaagg ccagcatgac
     2161 cgagctcgcg aagtcgccga aggtgaacgt gaaactcggc ggactggcca tgcccttccc
     2221 cggcttcccg ggcctcgacg gccaggcgag gccgtcgtcc caggcgctgg ccgacctgtg
     2281 gcggccctat gtggagacga ccatcgagct gttcggcgtc gaccgctgca tgttcgaaag
     2341 caacttcccg gtcgacctgt ggggctgcga cttcgccacc ctgtggaacg ccttcaaacg
     2401 catcgcggcg ggcgcgtcgg acgtggagaa ggcgtcgctg ttctcgggaa cggcccgccg
     2461 cgtctaccgg ctggcgtaga ggtccgccag cacggccggc gccaggcccg gcaggtcctc
     2521 ggcgatcagg ccggggccga agcggcgcgc ggcggcggca tggatccagg ccccggcgca
     2581 ggcggcctcg aaactgtcca tgccctgggc gataaggccg ccgatgaagc cggccagcac
     2641 gtcgccgctg ccggctgtg
//
LOCUS       DAORNX010000408         2657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29098_length_2657_cov_4.301691, whole genome shotgun sequence.
ACCESSION   DAORNX010000408 DAORNX010000000
VERSION     DAORNX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29098_length_2657_cov_4.301691"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLO65_14415"
     CDS             <1..186
                     /locus_tag="PLO65_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRD29490.1"
                     /translation="SRQTIAEAVASEAATRRSSAEGVNLDQELISMTTFQQAFNASAR
                     LVQASKDMYEALLVMVR"
     gene            190..1116
                     /locus_tag="PLO65_14420"
     CDS             190..1116
                     /locus_tag="PLO65_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRD29491.1"
                     /translation="MNRVATGNSYSTVLNDLMRAQVRQQEANAQVSSGKTASDLKGFS
                     RQAESLLATRSIQTKIDGFLTQGQTLTSKLESQDLALNQTADAAAGARQAIAEALATG
                     RGEALMGELSSWFSSASDSLNARFGGRYLFAGGQVDTPPVTAAAMADLTSQPSVAAFF
                     NNDNLAPASQLDESTTIQSGFLADQLGTGLFDAFRQVQAFVDANGDFTGQLSAAQSTF
                     LQGMLGQFETARSSLTDATARNGLIQNRVDKTMETQEARKAMLEGMVGGITDVDMAEA
                     ISRLQQAQTTVQASAQVFNTLSQSSLLDLLQP"
     gene            1169..1927
                     /locus_tag="PLO65_14425"
     CDS             1169..1927
                     /locus_tag="PLO65_14425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD29492.1"
                     /translation="MLKRVDYDEAQHAVYARGRQMPAGALARWMARFAEQLPAARPLT
                     ILDLGSGVGRLTPALAETFGGPVHGVEPSTKMREIAEATAAHPAVRYHAGEAARIPLP
                     DHSVDAVLLFLSFHHVPDRAAGAVEIARVLKPGGRVLVRSPFRDRMGGNWFYRYFPRA
                     EAIEKAMFPPLAEVTGVFADAGLAVKALVEVEEVYAANEAEAAERLKLRAISTFEHMT
                     EAEIEEGFARLDAKLALGADPNPSTGRSDLLVLG"
     gene            2035..>2264
                     /locus_tag="PLO65_14430"
     CDS             2035..>2264
                     /locus_tag="PLO65_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRD29493.1"
                     /translation="MNAHLPHTDIADDVVRAAVGLPAGSRVVAAMSGGVDSTVTAALL
                     ARAGYDVVGVTLQLYDHGAAIQKKGACCAGQD"
     assembly_gap    2265..2387
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2388..>2657
                     /locus_tag="PLO65_14435"
     CDS             <2388..>2657
                     /locus_tag="PLO65_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRD29494.1"
                     /translation="PCVRCNQTVKFRDLLDVARDLGAAAMATGHYVRREDGPGGPTLH
                     RAVDANRDQSYFLFATTREQLDYLRFPLGGLPKPAVRGVAAGMSLA"
BASE COUNT          394 a          935 c          862 g          343 t
ORIGIN      
        1 tcgcgccaga ccatcgccga ggccgtcgcc tcggaagcgg cgacccgccg cagctcggcc
       61 gagggcgtca acctcgacca ggaactgatc agcatgacca ccttccagca ggccttcaac
      121 gcctccgccc gcctcgtcca ggccagcaag gacatgtacg aggcccttct cgtgatggtg
      181 aggtaaggca tgaaccgcgt cgccaccggc aacagctatt ccacggtcct caacgacctg
      241 atgcgggccc aggtgcgcca gcaggaggcc aacgcccagg tctcgagcgg caagaccgcc
      301 tcggatctga agggcttttc ccgccaggcc gagtccctgc tggcgacccg ctcgatccag
      361 accaagatcg acggcttcct gacccagggc cagaccctga cctcgaagct ggagtcgcag
      421 gacctggcgc tcaaccagac cgccgacgcc gccgccggcg cccgccaggc catcgccgag
      481 gcgctcgcca ccggccgcgg cgaggcgctg atgggcgaac tgtcgagctg gttctcctcg
      541 gcctccgact cgctcaacgc ccggttcggc ggacgctacc tgttcgccgg cggccaggtc
      601 gacaccccgc cggtcacggc cgcggcgatg gccgacctga ccagccagcc gtcggtggcc
      661 gccttcttca acaacgacaa cctggccccg gccagccagc tcgacgagtc gacgaccatc
      721 cagtcgggct tcctcgccga ccagctgggg accggcctgt tcgacgcctt ccgccaggtg
      781 caggccttcg tcgacgccaa cggcgatttc accggccagc tgtcggcggc ccagtcgacc
      841 ttcctgcagg gcatgctcgg ccagttcgag acggcccgca gcagcctgac cgacgccacc
      901 gcccgcaacg gcctgatcca gaaccgcgtc gacaagacca tggagacgca ggaggcgcgc
      961 aaggcgatgc tcgagggcat ggtcggcggc atcaccgacg tcgacatggc cgaggccatc
     1021 agccgcctgc aacaggccca gaccacggtc caggcctccg cccaggtgtt caacaccctc
     1081 agccagagct cgctgctgga cctgctgcag ccgtaggttc tcacctggcg cggaattcgg
     1141 gctatgctgg cctggacgga ggcgcggcat gctcaagcgg gtcgactacg atgaagccca
     1201 gcacgcggtc tacgccaggg gccggcagat gccggccggg gcgctggcgc ggtggatggc
     1261 gcggttcgcc gagcagttgc cggccgcccg gccgctgacc atccttgacc tggggtcagg
     1321 cgtcggacgg ctgacgccgg cgctggccga gacgttcggc gggccggtcc atggcgtcga
     1381 gccgtcaacg aagatgcgcg agatcgccga ggccaccgcc gcccatccgg ccgtgcgcta
     1441 ccacgcgggc gaggccgccc gcattcccct gcccgatcac agcgtcgacg cggtgctgct
     1501 gttcctgtcc ttccaccatg tgccggaccg cgccgccggc gccgtcgaga tcgcgcgggt
     1561 gctgaagccc ggcggccggg tgctggtccg cagcccgttc cgcgaccgca tgggcggcaa
     1621 ctggttctat cgctacttcc cgcgcgccga ggccatcgag aaggcgatgt tcccgccgct
     1681 ggccgaggtg acgggcgtgt tcgcggacgc cggtctcgcg gtcaaggcgc tggtcgaggt
     1741 cgaggaggtc tacgccgcca acgaggccga ggccgccgaa cggctgaagc tgcgcgccat
     1801 ctcgaccttc gaacacatga ccgaggccga gatcgaggag ggattcgcac gcctcgacgc
     1861 caagctggcg ctgggcgcgg accccaaccc ctcgaccggt cggagcgacc tgctggtgct
     1921 gggctgaagt aaaggcgatg accgggacag ctggcggcga ggggcgaggc cgcgccgttg
     1981 ctgggctgaa cgccccaggt cgcaattccc ggcacgagcc tctaaatagc gccgatgaac
     2041 gctcacctgc cccacacgga catcgccgac gacgtcgtgc gcgccgcggt cggcctgccg
     2101 gccggcagcc gcgtggtcgc ggccatgtcg ggcggcgtcg actcgacggt gacggccgcg
     2161 ctgctggcca gggccggcta tgacgtggtc ggggtgacgc tgcagctcta cgatcacggg
     2221 gcggcgatcc agaagaaggg cgcctgctgc gccggccagg acatnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc ctgcgtgcgt
     2401 tgcaaccaga cggtcaagtt ccgcgacctg ctggatgtcg cccgcgacct gggcgccgcg
     2461 gccatggcca ccggccacta tgtgcgccgc gaggacgggc ccggcgggcc gaccctgcac
     2521 cgcgcggtcg atgcgaaccg cgaccagagc tacttcctgt tcgcgaccac gcgcgagcag
     2581 ctcgactacc tgcgcttccc cctcggcggc ctgcccaagc cggccgtgcg cggcgtggcg
     2641 gccggcatga gcctggc
//
LOCUS       DAORNX010000409         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29147_length_2653_cov_9.053888, whole genome shotgun sequence.
ACCESSION   DAORNX010000409 DAORNX010000000
VERSION     DAORNX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29147_length_2653_cov_9.053888"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..298
                     /gene="rpmB"
                     /locus_tag="PLO65_14440"
     CDS             2..298
                     /gene="rpmB"
                     /locus_tag="PLO65_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRD29495.1"
                     /translation="MSRRCELTGVGPMVGHNVSHSNIKTKRRFLPALSPATLQSEVLG
                     QSFKLRITNAALRTLDFRGGLDTFMAKAKDEDLSPRALKIKRQIKAKLAEKTAA"
     gene            complement(446..1585)
                     /locus_tag="PLO65_14445"
     CDS             complement(446..1585)
                     /locus_tag="PLO65_14445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRD29496.1"
                     /translation="MRPACVTDYRDLARRRLPRVLFDYLDGGSYAEATLRRNVEDFEA
                     LALRQRVMRDVSTISLETELFGQKMAMPMVLSPIGMGGMYARRGEVQAMKAARAAGLN
                     MCLSSLSICDVGEVTRDGGQAPWYQLYIIKDRGYMKELLGRVTELGCPVLAFTVDLPV
                     PGARYRDVRSGLFGAQTPFVPLQRLWDGLTHPGWAWDVMATGGPHTFGNLAPAIPEAK
                     GVMEFWPWVTKNFDPTLTWKDLDWIRENWDGPLVIKGILDADDAREARRYGADGLIVS
                     NHGGRQLDGVQSGIRALPPIADAVGDDLTVIMDGGVRSGLDVVKALASGAKGCFIGRA
                     WAWALAGAGQAGVEHVLQIMRQEMMMAMALTGCTDVKDAGRELLA"
     gene            complement(1582..2574)
                     /locus_tag="PLO65_14450"
     CDS             complement(1582..2574)
                     /locus_tag="PLO65_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRD29497.1"
                     /translation="MRRWAAVPGLLAALTLTLAACVTARVGLPAGPVAAGPTIAVQTT
                     PVLLDPADPARTTVGDFAYAGGVAIVSDATSRLHGLSDLLVNSTGGMTAVSDDGDLFT
                     GRLLLDPAGRLVGLTDAAMRSLTGPGDGPLQGKEWADAEGLTRLGDGAMLVSFERRHR
                     VWLYESMQQRRPTAVALPAVSMAENDGMEGLTAAPALGPDTYWVGLEPGGIWLCRIKL
                     SCDEVTGLPRPPPGYRLSSLATGPNGELVILHHSYIPTIGSRIIVTIVRDPTGAKRIA
                     GGFAMTPPLTVDNFEGVAVVARRQGPWRLYLLSDDNFSPTQRTLLLAFDWIPPE"
BASE COUNT          420 a          948 c          911 g          374 t
ORIGIN      
        1 catgtcgcgc cgctgcgaac tcaccggtgt tggtccgatg gtcggacaca acgtgagcca
       61 ctccaacatc aagaccaagc gccgcttcct gccggccctg tcgcccgcca cgctccagtc
      121 ggaagtgctg ggccagagct tcaagctgcg gatcaccaac gcggccctgc gcacgctgga
      181 cttccgcggc ggcctggaca ccttcatggc caaggcgaag gacgaggacc tctcgccccg
      241 ggccctgaag atcaagcgcc agatcaaggc caagctggct gaaaagaccg ccgcctgagg
      301 cgaaacaagc ttcaagacga cagacggccc gcggggggac tcgcgggccg tttgcgtttc
      361 gggggacatg ttccggcgat agccggtacg tgtcccctac ccaagcggct cgggtggggg
      421 acacgtacct gaaggtacat gtcccctaag ccagcagctc ccgcccggcg tccttcacgt
      481 cggtgcagcc ggtcagggcc atggccatca tcatctcctg gcgcatgatc tgcagcacat
      541 gctcgacgcc cgcctggccg gctcccgcaa gcgcccaggc ccaggcgcgg ccgatgaagc
      601 agcccttggc gccgctggcc agcgccttga ccacgtcgag ccctgaccgc acgcccccgt
      661 ccatgatcac cgtcaggtcg tcaccgaccg cgtcggcgat gggcggcagg gcgcggatgc
      721 cgctttgcac cccgtccagc tgccgaccgc catggttcga cacgatcagc ccgtcggcgc
      781 cgtagcgcct ggcctcgcgg gcgtcatcgg cgtcgaggat gcccttgatc accagcggcc
      841 cgtcccagtt ctcgcgaatc cagtcgagat ccttccaggt cagggtcgga tcgaagttct
      901 tcgtcaccca gggccagaat tccatcacgc ccttggcttc ggggatcgcc ggggcgaggt
      961 tgccgaaggt atgcggccca ccggtggcca tgacatccca ggcccagccc gggtgggtga
     1021 gtccgtccca cagtcgttgc agcggcacga acggcgtctg cgccccgaac aggccggacc
     1081 tgacgtcgcg atagcgcgcc ccgggcacgg gcaggtcgac ggtgaaggcc agcaccgggc
     1141 agccgagctc ggtcacccgg cccagcagct ccttcatgta gccgcggtcc ttgatgatgt
     1201 agagctggta ccagggcgcc tgaccgccat cgcgcgtgac ttccccgacg tcgcagatcg
     1261 acagcgacga caggcacatg ttcaggcccg cggcccgggc ggccttcatc gcctggacct
     1321 cgccgcgccg ggcgtacatg ccgcccatgc cgatcggcga caggaccatc ggcatggcca
     1381 tcttctggcc gaacaactcg gtctccagag agatcgtcga gacatcccgc atcacgcgct
     1441 ggcgcagggc cagggcctcg aagtcctcga cgttccgccg cagggtcgcc tcggcgtagg
     1501 agccgccgtc gagatagtcg aacaacaccc gcggcagccg ccggcgggcc agatcgcggt
     1561 agtcggtgac gcaggcgggt ctcattcggg cgggatccag tcgaaggcca gcagcagtgt
     1621 gcgctgcgtc gggctgaagt tgtcgtcgga aagcaggtag agccgccacg ggccctgacg
     1681 ccgggccacg acggcgacgc cctcgaaatt gtccacggtc aggggcggcg tcatggcgaa
     1741 gccgccggcg atccgcttcg cgcccgtggg gtcccgcacg atggtgacga tgatccgcga
     1801 gccgatcgtc ggaatgtagc tgtgatgcag gatgaccagt tcgccgttgg gtcccgtggc
     1861 caggctcgac aggcgatagc cgggcggcgg ccggggcagg ccggtcacct cgtcgcagga
     1921 cagtttgatc cggcacagcc atatgccgcc cggctcgagc ccgacccaat aggtgtccgg
     1981 gccgagggcg ggggcggcgg tcaggccctc catgccgtcg ttctcggcca tgctcacggc
     2041 gggcagggcg acggcggtcg gtcgccgctg ctgcatcgac tcgtacagcc agacccggtg
     2101 gcggcgctcg aaactgacca gcatcgcgcc gtcgcccagc cgggtcagtc cttccgcgtc
     2161 ggcccattcc ttgccctgca gcgggccgtc gcccggcccc gtcagcgacc gcatggcggc
     2221 gtcggtcagg ccgaccagac gcccggccgg atccagcaac agccgcccgg tgaacaggtc
     2281 gccgtcatcg gagacggccg tcatgccgcc ggtcgaattg accagcaggt cggacagtcc
     2341 gtgcagccgg ctggtcgcgt cgctgacgat cgccacgccc ccggcgtagg cgaaatcgcc
     2401 gaccgtcgtc cgcgccggat cggcggggtc gaggagcaca ggcgtggtct ggacggcgat
     2461 ggtcgggccg gccgccacgg gaccggcggg caggccgacc cgggcggtga cgcaggcggc
     2521 cagcgtcagg gtcagggcgg ccagcaggcc cggaaccgcc gcccatcgcc gcatcagccg
     2581 accgcctcgc gcacctggtc gaaggtcacc gtcttcggcg gcggcggctg ggtcacgggc
     2641 ggcgcggcca gca
//
LOCUS       DAORNX010000410         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29292_length_2642_cov_6.915733, whole genome shotgun sequence.
ACCESSION   DAORNX010000410 DAORNX010000000
VERSION     DAORNX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29292_length_2642_cov_6.915733"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLO65_14455"
     CDS             complement(<1..378)
                     /locus_tag="PLO65_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29498.1"
                     /translation="MRDGGRLAAAIQVLEDIETRHKPARMALKGWGESARYAGSKDRA
                     FVSGLVLDVLRKRRSLGWMMGEDSARAAALAAVRFGWGWPVERVAEAAGDSEHGIGGL
                     TEAEQASLAEPNALTMWEAQFGDF"
     gene            complement(393..788)
                     /locus_tag="PLO65_14460"
     CDS             complement(393..788)
                     /locus_tag="PLO65_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD29499.1"
                     /translation="MTFELSMLAWTLVLAFVQILAFDMARTGQYGLKWNTGARDEEMP
                     PLSPVANRLQRAQNNLFETLPLFIAAVLIAHVADRNSDLTHNGALIYLVGRIIYVPLY
                     AFGVKQIRSLVWLVSTAGLLMIFWAILAP"
     gene            complement(818..2281)
                     /gene="guaB"
                     /locus_tag="PLO65_14465"
     CDS             complement(818..2281)
                     /gene="guaB"
                     /locus_tag="PLO65_14465"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRD29500.1"
                     /translation="MEIREGLTFDDVLLEPGPSEVMPTQVSTVSRLTREISLNIPLVS
                     AAMDTVTESRLAIAMAQAGGLGILHRNLTIDEQADQVREVKRYESGMVINPLTINPDT
                     TLAEIREIKARRKISGFPVVEPTTGKLVGILTNRDMRFETDDRLKASALMTREGLITV
                     REGVDSGDALSLLKTNKIERLIVVDDEGRAVGLITVKDIDKATEHPHAAKDEKGRLRV
                     GAASTVGDAGFERSLALVEAGVDVVVIDTAHGHSRQVADAVSRLKRETNRVQIVAGNV
                     ATYDGARALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIAEASRAARDTDVPVI
                     ADGGIKNSGDLAKAIAVGASVAMMGSMFAGTEESPGEVFLYQGRSYKSYRGMGSLGAM
                     ARGSADRYFQKEVTDTFKLVPEGIEGQTPFKGPIAPVIHQLVGGLRAAMGYVGAPDIV
                     EFQKRAKFIRITGAGLRESHVHDVMITREAPNYPSAV"
     gene            2415..>2642
                     /locus_tag="PLO65_14470"
     CDS             2415..>2642
                     /locus_tag="PLO65_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29501.1"
                     /translation="MTGYDVRIAEPADGPMQRHPVMDTLTLAFSTDAPVRYMFPTAEG
                     FLRNFSRLSTAMAGSAVAAGTAWVADGGAAAA"
BASE COUNT          398 a          911 c          903 g          430 t
ORIGIN      
        1 gaagtcgccg aactgcgcct cccacatggt gagcgcgttg ggctcggcga gcgaagcctg
       61 ctccgcctcg gtcaggccgc cgatgccgtg ttcgctgtcg ccggccgcct cggcgacccg
      121 ctcgacaggc cagccccagc cgaagcggac ggcagcgagc gcggcggcgc gcgcgctgtc
      181 ctcgcccatc atccagccca gcgaccgccg cttgcgcagc acgtcgagga ccagtccaga
      241 gacaaaggcg cggtccttcg agcccgcgta ccgcgcggac tcgccccagc ccttgagcgc
      301 catgcgggca ggtttgtggc gcgtctcgat atcctcgagg acctggatcg ccgccgctag
      361 ccgcccgccg tcacgcaatc aaacacccga tatcagggag ccaggatggc ccagaagatc
      421 atcagcaggc ccgccgtgct gaccagccag accagtgagc ggatctgctt cacgccgaag
      481 gcgtagagcg gcacatagat gatccggccg accaggtaga tcagcgcgcc attgtgcgtc
      541 aggtcgctgt tgcggtccgc cacatgggcg atcagcacgg cggcgatgaa cagcggcagg
      601 gtctcgaaca gattgttctg cgcgcgctgc agccggttgg cgaccggcga caacggcggc
      661 atctcctcgt cgcgggcgcc ggtgttccac ttcaggccgt actggcccgt gcgggccatg
      721 tcgaaggcca ggatctggac gaaggccagg accagggtcc aggccagcat ggaaagctcg
      781 aacgtcatac ctcagtcccc cctgatgatc ccgaccgtca gacggcgctc gggtagttgg
      841 gcgcttcccg cgtgatcatc acgtcgtgga cgtggctctc gcgcaggccg gcgccggtga
      901 tgcggatgaa tttcgcccgc ttctggaact ccacgatgtc gggcgcgccg acatagccca
      961 tggccgcccg caggccgccg accagctggt ggatgaccgg ggcgatcggc cccttgaacg
     1021 gcgtctggcc ctcgatcccc tccggcacca gcttgaaggt gtcggtgact tccttctgga
     1081 agtagcggtc ggccgagccg cgggccatgg cccccagcga gcccatgccg cggtacgact
     1141 tgtaggaccg gccctggtag aggaacacct cgccggggct ttcctcggtg ccggcgaaca
     1201 tcgagcccat catcgccacg ctggccccga ccgcgatggc cttggccagg tcgccggagt
     1261 tcttgatgcc gccgtctgcg atcaccggca cgtcggtgtc gcgagcggcg cgagaagcct
     1321 cggcgatggc ggtcagctgc ggcaccccga ccccggccac gatgcgcgtg gtgcagatcg
     1381 agcccggtcc gatgccgacc ttcaccgcgt cggccccggc gtcgatcagg gccctggcgc
     1441 cgtcataggt cgccacgttg ccggcgacga tctgcacccg gttggtctcg cgcttgaggc
     1501 gcgacaccgc gtcggcgacc tgtctggaat ggccgtgggc ggtgtcgatg accaccacat
     1561 ccacgccggc ctcaaccagc gccagggagc gttcgaaccc cgcgtcgccg accgtcgagg
     1621 cggcgccgac ccgcaggcgg cctttctcgt ccttggccgc gtgcggatgc tcggtggcct
     1681 tgtcgatgtc cttgacggtg atcaggccga ccgcccggcc ctcgtcgtcg accacgatca
     1741 gccgctcgat cttgttggtc ttcagcaggc tgagcgcgtc gccggaatcg acgccctcgc
     1801 ggaccgtgat cagcccctcg cgggtcatca gggccgaggc cttgagccgg tcgtcggtct
     1861 cgaaccgcat gtcgcggttg gtcaggatgc caaccagctt gccggtggtc ggctcgacga
     1921 ccgggaagcc cgagatcttc cgccgagcct tgatctcgcg gatctcggcc agggtggtgt
     1981 cggggttgat ggtcagcggg ttgatgacca tgccgctctc gtaccgcttg acctcgcgca
     2041 cctggtcggc ctgttcatca atggtcaggt tgcggtgcag gatgccgagg ccgccggcct
     2101 gggccatggc gatggccagc cggctttcgg tcaccgtgtc catcgcggcg gacacgagcg
     2161 ggatgttcag actgatctca cgcgtaagcc gggacacggt cgacacctgg gtaggcatca
     2221 cttccgaggg accgggttcg agcaaaacat cgtcgaaggt gagcccttcg cgaatctcca
     2281 tgggagtctc cgttttgcgg gcgcggtatc gtggcaaagg ggggcgctgt caacggctgt
     2341 cgccggtggt gacggagact ggcggggcgg gccctgtctc cgctagggtc ccgacaggct
     2401 acgggaccat catcatgaca ggctatgacg tgcggatcgc ggagccggcc gacggcccga
     2461 tgcagcgcca cccggtgatg gacacgctca ccctggcgtt cagcacggac gcccccgtcc
     2521 gctacatgtt tccgacggcc gaaggctttc tgcgcaactt cagccggctc tcgaccgcga
     2581 tggccggcag cgccgtggcg gccggaaccg cctgggtcgc cgacggcggc gcggcggcgg
     2641 cg
//
LOCUS       DAORNX010000411         2638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29351_length_2638_cov_5.510647, whole genome shotgun sequence.
ACCESSION   DAORNX010000411 DAORNX010000000
VERSION     DAORNX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29351_length_2638_cov_5.510647"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLO65_14475"
     CDS             <1..772
                     /locus_tag="PLO65_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRD29502.1"
                     /translation="VLQGANLAGAQMTGAHAIAADFTEANLSGACLNGAKLINAVLDG
                     ADMSSAQVFHADLSGASMKDTVIAGVNLDDAGLDHVDLSEVLRAPPPIVYVDDRPLGD
                     LVNEHDLYCTSGGAEGATMKVSRIDFRPLKQLRRRKLTGMVATRSVFFGLNMEGAELQ
                     GSDLSGADLRGVVLRDADLRGAKLVGAKLNKADLRGARLGPLMIAEGRYLRTDLTRAV
                     LRGADLRDCHARRARFLEADLKGARIEGADLVGAELET"
     gene            complement(809..1672)
                     /locus_tag="PLO65_14480"
     CDS             complement(809..1672)
                     /locus_tag="PLO65_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRD29503.1"
                     /translation="MNAHTAETAAWTCFNVSIEDGVAHIQMKRPEAMNTMNKAFWAEL
                     PAIVRDINDNARARCIVISSTGKHFSAGMDLSVFANVGDSNAGPVDRHVAGESFRKHV
                     HALQDTFTCLDEARMPVIAAIQGGCIGGAVDFTSACDIRYCTADAFFTIMEINIGMTA
                     DVGTFPRLCKLIPEGWVRELAYTGRRLPAQRARDIGLVNEVFDSHEAVVAHALATARE
                     IAGKSPLAVAGSKVMINYARDHTIKDGLDYIALWQVGMFSGPHMAETFAAKQQGREPV
                     FPDLLPLRKGL"
     gene            1804..>2256
                     /locus_tag="PLO65_14485"
     CDS             1804..>2256
                     /locus_tag="PLO65_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HRD29504.1"
                     /translation="MRIAITASIAVLALAAVPGAANAGVFSCDANGGKQEGGAVIGAI
                     IGGVIGNQIAKGERGAGTVVGAGLGAAIGSSIGCKMQEEDQRRAEAALDQALATGQPA
                     RWRNPNTGAYGEIRVLPTSNSDYWYAPNTVNVRATPSTRGRVVGRMAGG"
     assembly_gap    2257..2375
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          393 a          862 c          893 g          371 t
ORIGIN      
        1 cgtgcttcag ggcgccaatc tggccggcgc ccagatgacc ggcgcccacg ccatcgccgc
       61 cgacttcacc gaggccaacc tgtcgggcgc ctgcctcaac ggggccaagc tgatcaacgc
      121 cgtgctcgac ggcgccgaca tgtcgagcgc ccaggtgttc catgccgacc tgtccggcgc
      181 ttcgatgaag gacaccgtga tcgcgggcgt caatctcgac gacgccggac tcgaccacgt
      241 ggatctcagc gaggtgctgc gggcgccgcc gccgatcgtc tacgtcgacg accggcccct
      301 gggcgatctg gtcaacgagc atgatctgta ctgcacctcc ggcggagccg agggcgcgac
      361 gatgaaggtt tcgcgcatcg acttccggcc gctcaagcag ctgcgtcggc gcaagctgac
      421 cggcatggtc gcgacccggt cagtgttctt cgggctcaac atggagggcg cggagctgca
      481 gggcagcgat ctgagcggcg ccgacctgcg cggggtagtg ctgcgcgacg ccgacctgcg
      541 cggagccaag ctggtcggcg ccaagctcaa caaggccgat ctgcgcggcg cccgtctggg
      601 gccgctgatg atcgccgagg ggcgctatct gcgcaccgac ctgacccggg cggtgctgcg
      661 cggcgccgac ctgcgcgact gccacgcccg ccgcgcccgc ttcctcgagg ccgacctcaa
      721 gggggcgcgg atcgaaggcg ccgatctggt cggggccgaa ctggagacgt gacccccgga
      781 tcctccgcgc gagcggggag cgccgacgtc aaagtccctt cctgagcggc aggaggtccg
      841 gaaacaccgg ctcgcggcct tgctgcttcg ccgcgaaggt ctcggccatg tgcgggcccg
      901 agaacatgcc cacctgccac agggcgatgt agtccaggcc gtccttgatg gtgtggtcgc
      961 gggcgtagtt gatcatcacc ttgctgccgg ccacggcgag cggcgacttg ccggcgatct
     1021 cgcgggccgt ggccagggcg tgggcgacca ccgcctcgtg gctgtcgaac acctcgttga
     1081 ccaggccgat gtcgcgcgcc cgctgggccg gcagacggcg accggtatag gccagctcgc
     1141 gcacccaccc ctcggggatc agcttgcaca gccgcgggaa ggtcccgaca tcggcggtca
     1201 tgccgatgtt gatctccatg atcgtgaaga aggcgtcggc ggtgcagtag cggatgtcgc
     1261 aggcgctggt gaagtcgacc gccccgccga tgcagccgcc ctggatcgcc gcgatcaccg
     1321 gcatgcgcgc ctcgtcaagg caggtgaagg tgtcctgcag ggcgtgcaca tgcttgcgga
     1381 aactctcgcc ggccacatgc cggtccaccg gcccggcgtt gctgtcgccg acattggcga
     1441 acaccgacag gtccatcccc gccgagaagt gcttgccggt cgaggaaatg acgatgcagc
     1501 gggcgcgggc gttgtcgttg atatcacgga cgatggccgg cagctcggcc cagaacgcct
     1561 tgttcatggt gttcatggct tcgggccgct tcatctggat atgcgccacc ccgtcctcga
     1621 tcgagacgtt gaagcaggtc caggcggcgg tttcggcggt gtgggcgttc atggttgctg
     1681 tcctcgcagg tattgacaca ttgtcgtgag atgacgtggg gaaagccaac cccggacgta
     1741 ttgaagtctg gaagcggcgt catgcctgca cgggcgccgt caggcatcat acgggagact
     1801 atcatgcgca tcgccatcac cgcctccatc gccgtcctcg ccctggccgc cgtcccgggg
     1861 gcggccaacg ccggcgtgtt cagctgtgac gccaacggcg gcaagcagga gggcggcgcc
     1921 gtcatcggcg ccatcatcgg cggggtcatc ggcaaccaga tcgccaaggg cgaacgcggc
     1981 gcgggcacgg tggtcggcgc cgggctgggg gcggccatcg gctcgtcgat cggctgcaag
     2041 atgcaggagg aggaccagcg ccgggccgag gccgcgctcg accaggccct ggccacgggc
     2101 cagccggccc gttggcgcaa cccgaacacc ggcgcctatg gcgagatccg cgtgctgccg
     2161 acgagcaaca gcgactactg gtatgcgccg aatacggtca acgtgcgggc cacgccctcg
     2221 acgcgcggcc gcgtggtcgg gcggatggcc ggcggcnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgcg aaacgctcga gcagacggcc
     2401 tacacgaaga aatacggtcg cgagaccgaa cgctaccgcg cgtgccgcaa gccgggcggg
     2461 gcctgggacg tgcggaagat ctgaggccta tcgactgacg ctgggtgagc gggctaggca
     2521 ggcggcgatg accaacaccg ccgcctcgcc gtttcccgcc gcgcgccgcc gcgccctcgc
     2581 cgtgcccggc ggcgccatcg ccggactgga gttcggtccc gccgaccggc ggctcgac
//
LOCUS       DAORNX010000412         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29379_length_2636_cov_6.835723, whole genome shotgun sequence.
ACCESSION   DAORNX010000412 DAORNX010000000
VERSION     DAORNX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29379_length_2636_cov_6.835723"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLO65_14490"
     CDS             <1..960
                     /locus_tag="PLO65_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRD29505.1"
                     /translation="WRGVAICAVAAAAATFLADHYRAPAMLMALLVGMALSPAASDER
                     CAEGVTFSARTLLRLGIALLGARITFGQIADLGVWPVVIAIGGVAATILVSIGLARLF
                     GFSPLFGVLTGGATGICGASAALALSAVLPAHPLKDRATTFTVVGVSLLSTAAMVLYP
                     LLATRLGLSERQAGLFLGGSIHDVAQVIGAGYSLSPTIGDIATVMKLVRVGALAPVVI
                     IVGLLSRKAGPDPDRPREPLLPWFAVAFLAIAAACSLGLIPAVIREAMTEASRWCLVL
                     AIAALGMRTSVASLVQVGVRPFALLAVEAAFLAAFILGAVHLL"
     gene            complement(1009..1359)
                     /locus_tag="PLO65_14495"
     CDS             complement(1009..1359)
                     /locus_tag="PLO65_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRD29506.1"
                     /translation="MAGHRLYAMPFASVYPAYVAKAERKGRTRAEVDEIILWLTGLNQ
                     DQLEAHLAAKTDFATFFAEAPRLNPDRAKITGVVCGVRVEQVADPLMREIRYLDKLVD
                     ELARGRPMEKILRA"
     gene            complement(1500..2249)
                     /locus_tag="PLO65_14500"
     CDS             complement(1500..2249)
                     /locus_tag="PLO65_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD29507.1"
                     /translation="MQTRVAEIADGVYRLSTFIPEVAPPGGLTFNQFLVTGDEPLLFH
                     TGLRGLFPLVHAAAARVIDPQRLRWITFGHYEADECGSMNDWLAAAPRAEVAHGQVGC
                     DVSVNDMADRPPRALADGEVIDLGGGKRVHYLETPHTPHGWDAGLLYEESTGTLLCGD
                     LFTQQQDGPALTEGDIVGPAIAAEDIFGYSALNPDMGRTLRRLADLNPRTLALMHGPS
                     FAGDGAAALRDLAADYDRRTLERRMAGPAAA"
     gene            2357..>2636
                     /locus_tag="PLO65_14505"
     CDS             2357..>2636
                     /locus_tag="PLO65_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD29508.1"
                     /translation="MARTYTLKRRAEQQAETRRRLAEAAMELHGTVGPARTSLSLVAE
                     RAGVQRNTLYAHFPDERSLFQACSALFIERNPPPNPAAWAAAGDPAARL"
BASE COUNT          331 a          938 c          939 g          428 t
ORIGIN      
        1 tggcgcggtg tcgcgatctg cgccgtggcg gccgcggccg cgaccttcct ggccgaccac
       61 tacagggcgc cggcgatgct gatggcgctg ctcgtcggca tggctctcag cccggccgcc
      121 tcggacgaac ggtgcgccga gggcgtgacg ttcagcgccc ggaccctgct ccggctcggc
      181 atcgccctgc tcggggcgcg gatcacgttc ggacagatcg ccgacctcgg cgtctggccg
      241 gtggtgatcg cgatcggcgg tgtcgcggcc accatcctgg tctcgatcgg cctggcccgg
      301 ctgttcggct tcagccccct gttcggcgtc ctgaccggcg gggcgacggg gatctgcggc
      361 gcatcggcgg cgctcgccct gtcggccgtg ctgccggcgc atccgctcaa ggaccgcgcc
      421 acgaccttca ccgtcgtcgg cgtgtcgctg ctgtcgacgg cggcgatggt gctctatccc
      481 ttgctggcca cgcggctggg cctgtccgag cgccaggccg gcctgttcct cggcggttcg
      541 attcacgacg tcgcgcaggt tatcggcgcc ggctacagcc tctccccgac catcggcgac
      601 atcgccacgg tgatgaagct ggtgcgcgtc ggcgccctcg cgccggtggt gatcatcgtc
      661 ggcctgctga gccgcaaggc cgggccggac ccggaccggc ccagggagcc gctgctgcca
      721 tggttcgccg tggcctttct cgccatcgcc gcggcctgca gcctgggcct gattccggcg
      781 gtcatccgcg aggcgatgac ggaggcctcc cgctggtgcc tggtcctggc catcgccgcc
      841 ctcggcatgc gcaccagcgt cgcctccctc gtccaggtcg gcgtgcggcc gttcgccctg
      901 ctggcggttg aggcggcgtt cctcgcagcg ttcatactcg gcgccgtcca cctgctctag
      961 ccgccccgga ggggaaggaa ccgcccccgc ccgccgatcc cgggccggtc acgcgcgcag
     1021 gatcttctcc atcggcctgc ccctggccag ctcgtcgacc agcttgtcga ggtagcggat
     1081 ctcgcgcatc agcgggtcgg cgacctgttc cacgcgcacg ccgcagacga cgccggtgat
     1141 cttcgcccgg tcggggttca gccggggcgc ctcggcgaaa aaggtcgcga aatccgtctt
     1201 cgccgcaagg tgcgcctcga gttggtcctg gttcaggccg gtcagccaga ggatgatttc
     1261 atcgacctcg gcccgcgtgc gacccttcct ttcggccttt gcgacatagg ccggatagac
     1321 gctggcgaac ggcatggcgt agaggcggtg tccggccatg gcgatccctg tcctgtcgtc
     1381 ggggctcctc acgccatgtc acggccgatg ccgccccggt gtcctggcag gcattcttgc
     1441 tccggccggg ccttggtcaa gcctttcggg cgtatcggcg cccctgtccg gtcgagaggc
     1501 taggcggcgg ccgggccggc catccggcgc tccagtgtcc gtcggtcgta gtccgccgcc
     1561 aggtcccgca gcgccgcggc gccgtcgccc gcgaaggacg gcccgtgcat cagcgccagg
     1621 gtccgcgggt tcaggtcggc cagtcgtcgc agggtcctgc ccatgtccgg attgagggcc
     1681 gaatagccga agatgtcctc ggccgcgatg gccgggccga cgatgtcgcc ttcggtcagg
     1741 gccgggccgt cctgctgctg ggtgaacagg tcgccgcaca gcagcgtgcc ggtggattcc
     1801 tcgtagagca ggcccgcgtc ccagccgtgc ggcgtgtggg gtgtctcgag gtagtggacc
     1861 cgcttgccgc cgcccaggtc gatgacctcg ccgtcggcca gggcgcgggg cggccggtcg
     1921 gccatgtcgt tgaccgaaac gtcgcagccg acctggccat gcgccacctc ggcccgcggc
     1981 gcggcggcca gccagtcgtt catgctgccg cactcgtcgg cctcgtagtg accgaaggtg
     2041 atccagcgca ggcgctgcgg gtcgatcacc cgggcggccg ccgcatgaac cagcgggaac
     2101 aggccgcgca gcccggtgtg gaacagcagg ggctcgtcgc cggtgaccag gaactggttg
     2161 aaggtcaggc cgcccggcgg ggcgacctcg ggaatgaagg tcgacaggcg atagacgccg
     2221 tcagcgatct cggcgacccg ggtctgcatg atgttgctcc ggttggacgg gctggaaaaa
     2281 tcacgataca tggtcgtgta gtcaaatgag gcgcggccgt caatatggct gcacattacc
     2341 gtttagtgaa aatcgcatgg cccgaaccta caccctcaaa cggcgcgccg agcagcaggc
     2401 ggaaacccgg agacggctgg ccgaggccgc gatggagctg cacggcacgg tcggcccggc
     2461 gcgcaccagc ctgagcctgg tcgccgagcg ggccggggtg cagcgcaata cgctgtatgc
     2521 ccacttcccg gacgagcgga gcctgttcca ggcctgttcg gccctgttca tcgagcgcaa
     2581 cccgccgccc aacccggcgg cctgggcggc ggcgggggac ccggccgcgc ggctgc
//
LOCUS       DAORNX010000413         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29554_length_2623_cov_8.119159, whole genome shotgun sequence.
ACCESSION   DAORNX010000413 DAORNX010000000
VERSION     DAORNX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29554_length_2623_cov_8.119159"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLO65_14510"
     CDS             <1..566
                     /locus_tag="PLO65_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRD29509.1"
                     /translation="TFMAEMVETAAILTQATPRSFVILDEIGRGTATWDGLAIAWACA
                     EALHGVNRSRALFATHYHELAALEQRLSAVSNLSMRAREWNGDLVFLHEAGPGHADRS
                     YGVQVAKLAGVPPAVVARAREVLERLESEAESPARLEGLPLFAAAAPTPVKHGPSPVE
                     EALKGLEPDGMSPREAMEALYRLKGML"
     gene            624..>2623
                     /locus_tag="PLO65_14515"
     CDS             624..>2623
                     /locus_tag="PLO65_14515"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRD29510.1"
                     /translation="MASRLRPTRLEHVVDGVALRARLTAAAHDASGNEGEQRRLALEI
                     LKAALFRGRMIAKERLENGAGGLETARLLCGVTDEVVTALYDFTTVHMFRARNPTEGE
                     RLCLLAVGGYGRGVLSPYSDLDLLFLRPYKQTAHAESVIEFMLYALWDLGFKVGHASR
                     TIEECVRLSKEDFTIRTSILEARRLTGDESLAEELKRRFRNDVVKNTGAEFVAAKLKE
                     RDDRHGRADASRYMVEPNVKEGKGGLRDLHTLMWIAEYLHPVDRPEDVFALAYFDRRD
                     VAAFVRAFDFLHAVRAHLHFATGRPEERLTFDLQPEIARRMGFGDRADNPAVERFMRR
                     YFLIAREVGALTRTFSAKLETEHLKAQPRGLSRFLPGRGGPKRKPLDVAGFHEEGGRL
                     NIEGPEVFERDPVDLIRIFRIADQRDLDLHPDAFTAVTRSLPLITVKTRRDPEAARAF
                     LDILARGKRTYRTLMLMNESGVLGRFVPEFGRIVAQMQFNMYHSYTVDEHTLKAVGVI
                     ADIADGRLAEDHPLSVSIMPLIDDRESLFLAMLLHDTGKGGAGGQEKAGARAARSACE
                     RLGLERSKIELIAWLVEHHLVMSDYAQKRDVTDPATVAAFARIVENPERLRLLLVLTV
                     ADIRAVGPGVWNGWKGQLMRELYAATEATFRGGRGSDPAASAR"
BASE COUNT          407 a          923 c          914 g          379 t
ORIGIN      
        1 cgaccttcat ggccgagatg gtcgagacgg ccgccatcct gacccaggcc accccgcgca
       61 gcttcgtcat cctcgacgag atcggccggg gcacggccac ctgggacggc ctggccatcg
      121 cctgggcctg cgccgaggcc ctgcacggcg tgaaccggtc gcgggcgctg ttcgcaaccc
      181 actatcacga gctggcggcg ctcgagcagc ggctgagcgc ggtctccaac ctgtccatgc
      241 gggccaggga gtggaacggc gacctggtgt tcctgcatga ggcgggcccc ggccacgccg
      301 accgcagcta cggcgtccag gtggccaagc tggccggggt gccgcccgcg gtcgtcgcgc
      361 gggcccgcga ggtgctcgaa cggctggaga gcgaggcgga gtcgccggcg cggctggaag
      421 gcctgcccct gttcgcggcg gcggccccga cgccggtgaa acacggcccc agcccggtcg
      481 aggaggccct caagggtctc gaaccggacg ggatgagtcc ccgggaggcg atggaagcgc
      541 tctatcgcct aaaaggcatg ctgtaggaag caacgcgggc cccacccgtt gcgccagccc
      601 gtgttgcagc gcacataaga cacatggcct cccgtctccg cccgacccgc ctcgaacatg
      661 tcgttgacgg cgtcgccctg cgcgcgcggc tgacggcggc ggcgcatgac gcctcgggca
      721 acgaaggcga gcagcggcgg ctggcgcttg agattctcaa ggcggccctg ttccgcggcc
      781 ggatgatcgc caaggagcgg ctggagaacg gcgccggcgg gctggagacg gcgcggctgc
      841 tgtgtggcgt caccgacgag gtggtcaccg ccctgtacga cttcaccacc gtgcacatgt
      901 tccgcgcccg caatccgacc gagggcgagc ggctttgcct gctggcggtg ggcggctacg
      961 gccggggcgt gctgtcgccc tacagcgacc tcgatctgct gtttctgcgc ccctacaagc
     1021 agacagcgca cgccgagagc gtcatcgagt tcatgctcta cgccctgtgg gatctgggct
     1081 tcaaggtcgg ccacgcctcg aggacgatcg aggaatgcgt gcggctctcg aaggaagact
     1141 tcaccatccg cacctcaatt ctcgaggcgc ggcgcctgac cggcgacgag tctcttgccg
     1201 aggagctgaa gcggcgtttc cgcaatgacg tggtgaagaa caccggggcc gagttcgtcg
     1261 ccgccaagct gaaggaacgc gacgaccgcc acggccgggc cgacgccagc cgctacatgg
     1321 tcgagcccaa cgtcaaggag ggcaagggcg ggctgcgcga tctgcacacc ctgatgtgga
     1381 tcgccgagta tctgcatccg gtcgaccggc ccgaggacgt gttcgccctg gcctatttcg
     1441 atcggcgcga cgtggccgcc ttcgtgcggg cgttcgactt cctgcacgcg gtccgggccc
     1501 acctgcattt cgccaccggc cggcccgagg agcggctgac cttcgatctg caaccggaga
     1561 tcgcccggcg aatgggcttt ggcgaccggg ccgacaaccc ggcggtcgag cggttcatgc
     1621 gccggtactt cctgatcgcc cgcgaggtcg gcgccctgac ccggaccttc tccgccaagc
     1681 tcgagaccga gcacctcaag gcccagccca ggggcctgtc gcgcttcctg ccgggccggg
     1741 gcggtcccaa gcgcaagccg ctcgacgtcg ccggcttcca cgaggagggc ggccgcctca
     1801 acatcgaagg gccagaggtc ttcgagcggg atccggtcga cctgatccgc atcttccgca
     1861 tcgccgacca gcgcgacctg gacctgcatc cggacgcctt caccgcggtg acccgcagcc
     1921 tgccgctgat caccgtcaag acccgccgcg acccggaggc ggccagggct ttcctcgaca
     1981 tcctggcgcg cggcaagcgg acctaccgca ccctgatgct gatgaatgag tccggcgtgc
     2041 tgggccggtt cgtgccggag ttcggccgga tcgtcgccca gatgcagttc aacatgtacc
     2101 acagctacac ggtcgacgag cacacgctga aggccgtcgg ggtgatcgcc gacatcgccg
     2161 acggacggct ggccgaggac catccgctgt cggtctcgat catgcccctg atcgacgatc
     2221 gcgagagcct gttcctggcc atgctgctgc acgacaccgg caagggtggg gccggcggcc
     2281 aggaaaaggc cggcgcccgc gcggcccgct ccgcctgcga gcggctggga ctggagcgga
     2341 gcaagatcga gctgatcgcc tggctggtcg agcaccacct ggtgatgagc gactacgccc
     2401 agaagcgcga cgtgaccgat ccggcgacgg tcgcggcctt cgcccgcatc gtcgagaacc
     2461 ccgagcgact gcggctgctg ctggtgctga cggtggccga catccgcgcc gtcggtccgg
     2521 gcgtgtggaa cggctggaag ggtcagctga tgcgcgagct ctatgccgcc accgaggcga
     2581 ccttccgcgg cggccgcggc tccgatccgg ccgccagcgc ccg
//
LOCUS       DAORNX010000414         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29555_length_2623_cov_4.931075, whole genome shotgun sequence.
ACCESSION   DAORNX010000414 DAORNX010000000
VERSION     DAORNX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29555_length_2623_cov_4.931075"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2507)
                     /locus_tag="PLO65_14520"
     CDS             complement(<1..2507)
                     /locus_tag="PLO65_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRD29511.1"
                     /translation="MHIRIKAALAVLAAVLVGATWTLAGAREPVQPLPSLSEPSLSPD
                     GQTIAFASGGDIWTVPAAGGVARLLVTDRATESRPLYSPDGTRLAFVSTRGGLANLYV
                     MTLATGAVTRLTYADANESLDGWSRDGRWIYFTSGVNDVQRQGDIFRVSADGGTPLEV
                     SRERYLNEFQSAPSPDGRSIALMARGISSGQWWRHGSSHIDQTELWLKPIADAGSYRR
                     LLPSGARHAWPMWRPDGQALYYMSDEGGAENLWRLTLGGQAERITRFTDGRVLWPTIG
                     YDGKAIVFERGFAIWMLDTATGQARQVPVTLQGAPAAAGESRLDLSSFDDLALSPDGK
                     KLAVLAHGELFATTAKDGGPAQRVTRTPGVERDAVWSPDSKRLLYVSERGLSANLYAW
                     DFASGVETALTTGLDRNTVPAWSPDGRMIAYVRAARELRVITLTLAGAVARDVAIYDG
                     PLGGYEDVAPVWSPDSQWLAFTVTDRKAFDNVHVIPAAGGAARPVSFLANGQTGGRIV
                     WSPDGKYILTDTGQRSEEGKLLRIDLLPHTPKYREDAFRDLFKPATTPESPAPAPETS
                     DGAPTADESAPATTPGKPGKPAKPGAAAKPAKKPEPVRIVFEGIRERASFLPLGMDVG
                     LPAISPDGKTLLFAAQAAGAVNYYTWNLDELAKEPPVPVQLTSSRKDKGAAVFTPDSK
                     SIFYLEGGTVLSTPVESPKPKPLAVNAEMEVDFDVEKMVVFDQAWGILDRGFYDPKFH
                     GRDWKAIRAAWTPYIAGARTGDELRRDINLMIGELNASHSGIRGPDAPSTPGRVGDLG
                     LRFDREAAEAGRGLVIREVVVLGPAAIEGSIKVGETLV"
BASE COUNT          361 a          915 c          961 g          386 t
ORIGIN      
        1 accagggtct cgccgacctt gatgctgccc tcgatggcgg ccgggccgag caccaccacc
       61 tcacggatca ccaggccgcg ccccgcctcg gccgcctcgc ggtcgaagcg caggcccagg
      121 tcgccgaccc tgcccggcgt ggatggggcg tccggcccgc ggatgccgct gtgcgaggcg
      181 ttcagctcgc cgatcatcag gttgatgtcg cggcgcagct cgtcgcccgt gcgggccccg
      241 gcgatgtagg gggtccaggc cgcgcggatg gccttccagt cgcggccgtg gaacttcgga
      301 tcatagaagc cgcggtcgag gatgccccag gcctggtcga acacgaccat cttctcgacg
      361 tcgaaatcga cctccatctc ggcgttgacc gccagcggct tgggcttggg cgactcgacc
      421 ggggtcgaca gcacggtccc gccctcgagg tagaagatcg acttcgagtc cggggtgaac
      481 accgccgcgc ccttgtcctt gcgcgaggag gtcagctgca ccggaaccgg cggctccttg
      541 gccagctcgt cgaggttcca ggtgtagtag ttgaccgccc ccgcggcctg ggccgcgaac
      601 agcagggtct tgccgtcggg gctgatggcg ggaagcccga cgtccatgcc cagcggcagg
      661 aaggaagccc gctcgcggat gccctcgaag acgatgcgca cgggctcggg cttcttcgcg
      721 ggcttcgcgg ccgcgccggg cttggcgggc ttgccaggtt tgccgggagt cgtggccggc
      781 gcggactcgt cggccgtcgg ggcgccgtcc gaggtctccg gcgcgggggc cgggctctcg
      841 ggcgtggtcg ccggcttgaa caggtcgcgg aacgcgtcct cgcggtattt cggcgtgtgc
      901 ggcagcaggt cgatgcgcag cagcttgccc tcctcgctgc gctggcccgt gtcggtcagg
      961 atatacttgc cgtcgggcga ccagacgatc cgaccgccgg tctggccgtt ggccaggaag
     1021 ctgaccggcc gggccgcgcc gccggcggcc gggatcacat ggacgttgtc gaacgccttg
     1081 cggtcggtga ccgtgaaggc cagccactgg ctgtcgggcg accagaccgg ggccacgtcc
     1141 tcatagccgc cgagcgggcc gtcgtagatg gccacgtccc tggccactgc gccggccaga
     1201 gtcagggtga tcacccgcag ctccctggcc gcccggacat aggcgatcat ccttccgtcc
     1261 ggcgaccagg ccgggacggt gttgcggtcc aggccggtgg tcagggccgt ctcgacgccg
     1321 ctggcgaagt cccaggcgta gaggttggcc gacaggccgc gctcggagac atagagcagg
     1381 cgcttggagt cgggcgacca gaccgcgtcg cgttcgactc ctggcgtgcg ggtcacgcgc
     1441 tgggccgggc cgccgtcctt ggccgtggtc gcgaacagct cgccatgggc cagcacggcc
     1501 agcttcttgc cgtcgggcga cagggccagg tcgtcgaagc tcgacaggtc cagccggctc
     1561 tcgccggccg cggccggggc tccctgcaga gtgaccggca cctggcgcgc ctggccggtc
     1621 gccgtgtcga gcatccagat cgcgaagccg cgttcgaaca cgatggcctt gccgtcgtag
     1681 ccgatcgtcg gccacagcac ccggccgtcg gtgaagcggg tgatccgttc ggcctggccg
     1741 cccagcgtca gccgccagag gttctcggcc ccgccctcgt cgctcatgta gtagagcgcc
     1801 tggccgtccg gccgccacat cggccaggcg tgccgcgcgc cggatggcag cagccgccgg
     1861 taggagccgg cgtcggcgat cggcttgagc cacagttcgg tctggtcgat gtggctgctg
     1921 ccatgacgcc accactggcc gctgctgatg ccgcgggcca tcagggcgat cgaccggccg
     1981 tccggcgagg gcgcgctctg gaactcgttg agatagcgct cgcggctgac ctccagcggc
     2041 gtgccgccgt cggcgctgac gcggaagatg tcgccctggc gctgcacgtc gttgacgccc
     2101 gaggtgaagt agatccagcg gccgtcgcgc gaccagccgt cgagcgactc attggcgtcg
     2161 gcataggtca gccgcgtcac cgcgccggtg gccagggtca tgacatagag attggccagg
     2221 ccgccacggg tcgagacgaa ggccagccgg gtcccgtctg gcgaatacag cggccgcgac
     2281 tcggtggccc ggtcggtgac cagcagccgc gccacgccgc cggccgccgg cacggtccag
     2341 atgtcgccgc ccgaggcgaa ggcgatggtc tggccatcgg gcgacagcga gggctcgctg
     2401 agcgagggca ggggctgcac cggttcgcgc gccccggcca gggtccaggt cgcgccgacg
     2461 aggacagcgg cgagcacggc cagggcggcc ttgatccgga tatgcatgaa actgtccccc
     2521 acctgacgct gccaacgccg cgaccgcgaa ccctcccgca ggatcgccgg cgaggaaagg
     2581 ggaagcggct gaccgccgga ttacaatttg ctcatgctag ccg
//
LOCUS       DAORNX010000415         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29632_length_2617_cov_6.294301, whole genome shotgun sequence.
ACCESSION   DAORNX010000415 DAORNX010000000
VERSION     DAORNX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29632_length_2617_cov_6.294301"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>342)
                     /locus_tag="PLO65_14525"
     CDS             complement(<1..>342)
                     /locus_tag="PLO65_14525"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522310.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRD29512.1"
                     /translation="KAAWEAGRPQVVFRRLIDDLETPVSAYLKIARDRPYAFLFESVE
                     GGAWRGRYSIVAMKPDLVWRCRGDVAEVAEGEAIAADRFTAQAGGALDSLRDLVARSR
                     IELPAGLPPPA"
     assembly_gap    343..403
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<404..2302)
                     /locus_tag="PLO65_14530"
     CDS             complement(<404..2302)
                     /locus_tag="PLO65_14530"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRD29513.1"
                     /translation="MLAFMRKFAKSWLALLLFVPLLVGFAVFGVNRDILGGAGSNYVI
                     KAGSRTITAYDFKREFDSYKTQLEQRFQQAISPELAQQQGLDRRVLEELAFRESLDEL
                     LSRMGLRASDKMLKDELRKAPVFFDSVTGEFDENKYAQALREKGLTPAVFEGILTDEM
                     RRNQFVAAAVAGLRAPRAYSAIGGLFALEQRDLAYFTVTPASVGPVAEPTEAEINAFI
                     KENAARLTMPEFRAFTVVHFSAKDLEKTVTVDEAEVQKQFAFMKDSLSKPELRSVTQI
                     PAKDAAQAAAIIARLQKGEDPSAVARSVGVTAVSYADKPRTAFFDPAIAAAAFSLPEG
                     AVSAPLKGQFGLAVIKVTKVVAGKEATLEEHRAEIVAKVRGDLAGAKISDLSKVYEDA
                     HEAGATLPEAARKAGVPTTVYAPVTADGRGQDGKPVAGVSPQVLKAVYELPQGGESDI
                     QEAGEGDYFAVRVDRIVPPAMPPMASIRPQMIRTLMLKTQADRMKARAEALVARIRKG
                     ETLEAVAASAGAPVVRVSGISRATLQAHQALGRDLVGGVLGARKGEAFSASAPDFSVA
                     VAVVTAIRTGDPQQVAQATEQARQQFTQELFGDIGEAARSYARTRLKTKADLTRARIA
                     IGIDPAVAA"
     gene            2462..>2617
                     /locus_tag="PLO65_14535"
     CDS             2462..>2617
                     /locus_tag="PLO65_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRD29514.1"
                     /translation="MTASRPLIAGNWKMNGLAASLDEARAVAAALDARPPGARVAICP
                     PATLVHRL"
BASE COUNT          361 a          882 c          915 g          398 t
ORIGIN      
        1 cggccggcgg cggcaggccg gccggtagtt cgatgcgcga gcgggccacc agatcgcgta
       61 ggctgtccag cgccccgccg gcctgcgccg tgaagcgatc ggcggcgatg gcctcgccct
      121 cggcgacctc ggcgacgtcg ccgcggcagc gccagaccag gtccggcttc atcgccacga
      181 tggaatagcg gccgcgccat gcgccgccct cgaccgattc gaacaggaag gcgtagggcc
      241 ggtcacgggc gatcttcaga taggccgaga ccggcgtctc cagatcgtcg atcagccggc
      301 gaaagacgac ctggggccgt cctgcctccc atgccgcctt gannnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccgcc acggccggat
      421 cgatgccgat ggcgatgcgg gcgcgggtca gatcggcctt ggtcttgagg cgggtgcggg
      481 cgtagctgcg ggccgcctcg ccgatgtcgc cgaacagttc ctgggtgaac tgctggcgcg
      541 cctgctcggt ggcctgggcc acctgctgag gatcgccggt gcgaatggcg gtgaccacgg
      601 ccaccgcgac gctgaagtcc ggcgcgctgg ccgagaaggc ctcgcccttc cgggccccga
      661 gcacgccccc gaccagatcg cgaccgagcg cctggtgggc ctgcagggtg gcgcggctga
      721 tgccgctgac ccggaccacc ggcgcgccgg ccgaggcggc cacggcttcc agggtttcgc
      781 ccttgcggat ccgggcgacc agcgcctcgg cgcgggcctt catgcggtcg gcctgggtct
      841 tgagcatgag ggtgcggatc atctgcggcc ggatcgacgc cataggcggc atggccggcg
      901 gcacgatgcg gtcgacgcgg accgcgaaat agtcgccctc gccggcctcc tggatgtcgc
      961 tttcgccgcc ctgcggcagt tcgtagaccg ccttgagcac ctgcggcgag acgccagcga
     1021 ccggcttgcc gtcctggccg cgtccgtcgg cggtcaccgg cgcatagacg gtggtcggca
     1081 cgccggcctt gcgggcggct tcgggcaggg tggcgcccgc ctcgtgggcg tcctcgtaga
     1141 ccttggacag gtcggagatc ttcgccccgg ccaggtcccc gcgcaccttg gccacgatct
     1201 cggcccggtg ctcctcgagg gtggcctcct tgccggcgac gaccttggtc accttgatca
     1261 cggccaggcc gaactgcccc ttcagcggcg cgctgacggc gccttcgggc agcgagaagg
     1321 ccgccgccgc gatggctggg tcgaagaagg cggtgcgcgg cttgtcggcg taggacaccg
     1381 ccgtcacgcc gacggacctg gccacggcgg acggatcctc gcccttctgc agccgggcga
     1441 tgatcgccgc ggcctgggcg gcgtccttgg ccgggatctg cgtcaccgag cgcagctcgg
     1501 gcttggacag gctgtccttc atgaaggcga actgcttctg cacctcggcc tcgtcgaccg
     1561 tcacggtctt ctcaaggtcc ttggccgaga agtgcaccac ggtgaaggcg cggaactccg
     1621 gcatcgtcag ccgggcggcg ttttccttga tgaaggcgtt gatctcggcc tcggtcggct
     1681 cggcgaccgg tccgacgctc gccggcgtga cggtgaaata ggccaggtcg cgctgctcga
     1741 gcgcgaacag gccgccgatc gccgaatagg cccgcggcgc ccgcaggccc gccacggcgg
     1801 cggcgacaaa ctggttgcgg cgcatctcgt cggtgaggat gccctcgaac accgccgggg
     1861 tcaggccctt ctcgcgcagc gcctgggcgt acttgttctc gtcgaactcg ccggtcactg
     1921 aatcgaagaa caccggcgcc ttgcgcagtt catccttcag catcttgtcc gaggcgcgca
     1981 gccccatccg ggacagcagt tcgtccagcg attcccggaa cgccagttcc tcgagcacac
     2041 ggcggtcgag gccctgttgc tgcgccagtt cgggcgagat ggcctgctgg aaacgctgtt
     2101 caagctgggt cttgtagctg tcgaactcgc gcttgaagtc gtaggccgtg atcgtgcggc
     2161 tgccggcctt gatcacatag ttggatcccg cgccgccgag gatgtcgcgg ttcacgccga
     2221 acacggcgaa tcccaccagc agcgggacga acagcaggag ggcgagccag gatttggcga
     2281 acttccgcat aaaggcgagc atgaaaggac agaccctcag gaacgcgggg cgcttgcgcc
     2341 gccccggcgg tatagtggag gcgcatccaa ccccgcaagc gtggcgccga tgcggcgtga
     2401 cagggcggtc gccttcgcgt agggcgcggg acgggatttc tgaaacgacg gggctttgtc
     2461 gatgaccgct tcgcggccgc tgattgctgg aaactggaag atgaacgggc tggccgcgtc
     2521 actcgacgag gcccgcgccg tcgccgccgc cctcgacgcc aggccgccgg gcgcgcgggt
     2581 ggccatctgt ccgccggcga ccctggtcca ccgtctg
//
LOCUS       DAORNX010000416         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29666_length_2615_cov_7.562109, whole genome shotgun sequence.
ACCESSION   DAORNX010000416 DAORNX010000000
VERSION     DAORNX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29666_length_2615_cov_7.562109"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLO65_14540"
     CDS             <1..493
                     /locus_tag="PLO65_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HRD29515.1"
                     /translation="ATPLPGFDKLADERAPPPDWPRFPGRPAAILVDGWCLGATRQDP
                     ATLAAPINALEREEDPNAVWRGYADEALAGRYQAFFGGFDAIVFLAAPSFDSVLAWRC
                     EQEAGLLAMAPGDLPAERRGEIARFIQHFERITRHMLAGGVAATATVALDPDRRPGAL
                     PDR"
     gene            545..1360
                     /locus_tag="PLO65_14545"
     CDS             545..1360
                     /locus_tag="PLO65_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29516.1"
                     /translation="MDKFDNILALISAAGAFGIAAYGLVEALGKAIAWRGRPSRFSKA
                     GVFGLPFVGFNEIRKLAGNLEPALRRTYGPDFMDILVQQYRNGRSKGEAPSTLRQGTL
                     LALPFMPPGEVAGVIDNIWGMGGGAAAIRQGSYADRLALALTEEKAANESARDDFTPT
                     ANDAALAGRFTAALDTRIQAAFAVAEESYGAVLRLWSGIAAIGLAVSFNSITGGQMLT
                     LVGIQDPEPLAGFIAAAIFGAVSVPLAPVANDIVSALSEAMKAWRSVSAVKRT"
     gene            1370..2386
                     /locus_tag="PLO65_14550"
     CDS             1370..2386
                     /locus_tag="PLO65_14550"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29517.1"
                     /translation="MASTPVFISFRASGLGGGVTDAQLFRIEPFAGYRWRDWTYAAHQ
                     RTDLKPLPLMSAERSAPLQGREVWFLVHGFNTDRDRGFMSLGAAVQEAMGLGVRTDPL
                     FSPVDNLIPVLWPGDSAGFAPTRPLGYPNEIEDVRETAKHFARLLLDPRTRPARINFV
                     THSLGARVALDTLRLVTADANAPQVGTVLLMAAAADDDILDDPRFDGAVRAIERIIVL
                     SSDTDRVLTWAFPPGDVLEAALHQGERPSLHALGKFGPRLKKDSPAAGKVEWRHHRVV
                     QDHNDYNAWPWNFPSKDNGWNASRWRTHRFIRRAAIDYDPAHPDAWQEPDRPIVARVP
                     RVSA"
     gene            complement(2400..>2615)
                     /locus_tag="PLO65_14555"
     CDS             complement(2400..>2615)
                     /locus_tag="PLO65_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRD29518.1"
                     /translation="QDFGDLLKSVMTDAMQASKTAETQMAAQVSGKAELIDVVTAISS
                     AEASLETVMAVRDQVISAYQEIMRMPI"
BASE COUNT          389 a          956 c          881 g          389 t
ORIGIN      
        1 cgccacgccc ctgcccgggt tcgacaagct ggccgacgag cgggcgccgc ccccggactg
       61 gccccgtttt ccgggccgac ccgcggccat cctggtcgat ggatggtgcc tgggcgcgac
      121 acgtcaggac ccggcgaccc tcgccgcccc gatcaacgcc ctggagcgcg aggaagaccc
      181 gaacgccgtc tggcgcggct acgccgacga ggccctggcc ggccgttacc aggccttctt
      241 cggcgggttc gacgcgatcg tctttctcgc cgccccttcg ttcgacagcg tgctcgcgtg
      301 gcgctgcgaa caggaggccg ggctgctcgc aatggcgccg ggcgatctgc ccgcggagcg
      361 ccgcggggag atcgctcgct tcatccagca cttcgagcgc atcacgcgcc acatgctggc
      421 cggcggagtc gccgccacgg cgaccgttgc gctggatccg gaccgacgac ccggcgcctt
      481 gcccgaccgc tgaattcccc ttatggttga gtgagacttg gcgacatcgc gcgacgaggg
      541 cgacatggac aagttcgaca acatcctggc gctgatctcg gcggccggcg ccttcggcat
      601 cgcggcctac ggcctggttg aagccctcgg caaggcgatc gcctggcgcg gccggccgag
      661 ccggttcagc aaggcgggcg tgttcggcct gccgttcgtc ggcttcaacg aaatccgcaa
      721 gctggccggc aacctggagc cggccctgcg ccgcacctac gggccggact tcatggacat
      781 cctggtccag cagtatcgca acggtcgcag caagggcgag gccccctcga ccctgcgaca
      841 gggaacgctg ctggcgctgc cgttcatgcc ccccggcgag gtcgccgggg tcatcgacaa
      901 catctggggc atgggcggcg gcgcggcggc catccggcaa ggcagctatg ccgaccggct
      961 ggccttggcc ctgaccgagg aaaaggcggc caacgaatcc gcccgggacg acttcacccc
     1021 cacggccaat gacgccgccc tggccggccg cttcaccgcc gcgctcgaca cccgcatcca
     1081 ggccgccttc gccgtggccg aggaaagcta cggcgccgtg ctgcgcctgt ggtccggcat
     1141 cgccgcgata ggcctggccg tcagcttcaa cagcatcacc ggcggccaga tgcttacgct
     1201 ggtcggcatc caggaccctg agcccttggc gggcttcatc gccgcggcga tcttcggagc
     1261 cgtctcggtg ccgctggccc cggtggccaa cgacatcgtc agcgccctgt ccgaagccat
     1321 gaaggcctgg cgcagcgtca gcgccgtcaa gcggacgtag gcgcgcccga tggcatcgac
     1381 gccggtcttc atcagctttc gcgccagcgg cctcggcggc ggcgtcaccg acgcccagct
     1441 gttccgcatc gagcccttcg ccggctatcg ctggcgggac tggacctacg ccgcgcacca
     1501 gcggaccgac ctgaagccgc tgcccctgat gtcggccgaa cgcagcgcgc cgctgcaggg
     1561 ccgcgaggtc tggttcctgg tgcatggctt caacaccgac cgcgaccggg gcttcatgtc
     1621 gctgggcgcc gccgtgcagg aggccatggg gctgggcgtc cggaccgatc cgctgttcag
     1681 ccccgtcgac aacctgatcc ccgtgctctg gcccggcgat tcagccggct tcgccccgac
     1741 ccggccgttg ggctatccca acgagatcga ggacgtgagg gagacggcga agcacttcgc
     1801 caggctgctg ctggatccga gaacccggcc ggcgcggatc aacttcgtca cccacagcct
     1861 gggcgcgcgg gttgcgctgg ataccctgcg cctggtgacc gccgacgcca acgcgccgca
     1921 ggtcgggacc gtgctgctga tggccgccgc ggccgacgac gacatcctcg acgacccgcg
     1981 gttcgacggg gccgtcaggg ccatcgagcg gatcatcgtg ctcagttccg acacggaccg
     2041 ggtcctcacc tgggccttcc cgcccggcga tgtgctggag gccgccctgc accagggcga
     2101 gcggccgtcg ctccatgccc tgggcaagtt cgggccaagg ctgaaaaagg actcgccggc
     2161 cgccggcaag gtcgaatggc ggcaccacag ggtcgtgcag gaccacaacg actacaatgc
     2221 ctggccctgg aacttcccca gcaaggacaa tggctggaac gcctcgcgct ggcgcaccca
     2281 ccggttcatc cgcagggcgg cgatcgacta tgacccggcg catccggacg cctggcagga
     2341 gcctgaccga ccgatcgtcg cccgcgtccc ccgggtcagc gcctgagggg cggccgctgc
     2401 tagatcggca tccgcatgat ttcctggtag gccgagatta cctggtcacg gacggccatg
     2461 accgtctcga ggctggcctc ggccgaggag atggcggtga ccacgtcgat cagttcggcc
     2521 ttgcccgaga cctgggcggc catctgggtc tcggcggtct tcgacgcctg catggcgtcg
     2581 gtcatcaccg acttgagcag atcgccgaaa tcctg
//
LOCUS       DAORNX010000417         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29721_length_2612_cov_3.960892, whole genome shotgun sequence.
ACCESSION   DAORNX010000417 DAORNX010000000
VERSION     DAORNX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29721_length_2612_cov_3.960892"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLO65_14560"
     CDS             <1..380
                     /locus_tag="PLO65_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRD29519.1"
                     /translation="GAAAMPRPAMPATTPERLAMMEKRMAEHQAMFRKHNDAIRAFYG
                     QLSPSQQKAFDALHMGRGMGGMHGPGVRRMRMIHRAGPGGPGAPMAMEEVGGPGDMMF
                     MGDPGDDEDIEIELGEGPEAPDQ"
     gene            426..1148
                     /locus_tag="PLO65_14565"
     CDS             426..1148
                     /locus_tag="PLO65_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HRD29520.1"
                     /translation="MFAFLANPDRFMAFSRRAAPAFGALAAALIAVGLWFSFAAPEDY
                     QQGDTVRIMFIHVPAAYLAMFSYICLGVASFLSLVYRHALADAAARACAPLGAAFTAL
                     ALVTGALWGKPMWGTWWVWDARLTSVLVLFLFWLGYMALRAAIDDETKAGRAAAILAL
                     VGLINLPVIHYSVEWWNSLHQGATVFAAKAENRLPPAYLIPMALMALGYTSAFGSLWL
                     VRIRAEVWRRRAAVLALQAAEG"
     gene            1154..1306
                     /gene="ccmD"
                     /locus_tag="PLO65_14570"
     CDS             1154..1306
                     /gene="ccmD"
                     /locus_tag="PLO65_14570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HRD29521.1"
                     /translation="MLDFDAGKYAAYVWPSFIITAVAFGWMIADSLARARRWKREVEA
                     RGKDRP"
     gene            1303..1818
                     /locus_tag="PLO65_14575"
     CDS             1303..1818
                     /locus_tag="PLO65_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HRD29522.1"
                     /translation="MRRWLAFVPLVALVLLGVLFAGYSLKRNPNVQPAALVGRPVPDL
                     ALPALETGRPTPVRQAAPGPLLINFYASWCAPCELEAPALMRLRGQGVIILGIGYKDA
                     PENNRAFLGRVGDPYAARLLDRDGRAGLEFGVTGVPESFLVDASGVITAKFTGELTPA
                     EEREIVAATRR"
     gene            1879..2166
                     /locus_tag="PLO65_14580"
     CDS             1879..2166
                     /locus_tag="PLO65_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29523.1"
                     /translation="MTPVRPNVWANVPPPAQGSGQGAARSAAQRAFFDAALGKAATTT
                     PAQPQTAPAKAVPLQTQAPSAPQPLQRTPSAQPDEPPARILRPGSLLDIRV"
     assembly_gap    2231..2400
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2401..>2612)
                     /locus_tag="PLO65_14585"
     CDS             complement(<2401..>2612)
                     /locus_tag="PLO65_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD29524.1"
                     /translation="ALAEIGPRADAADARLLALLPKGKRGAFLEALAALSSVAAPTRE
                     KKIKPPKADKPAKPEKAAKAPKPTRP"
BASE COUNT          314 a          890 c          856 g          382 t
ORIGIN      
        1 ccggcgccgc ggcgatgccg cgcccggcca tgcccgccac cacgcccgag cggctggcca
       61 tgatggaaaa gaggatggcc gaacaccagg ccatgttccg caagcacaat gacgccatcc
      121 gcgccttcta cggccagctc agcccgtcgc agcagaaggc cttcgacgcc ctgcacatgg
      181 gccgcggcat gggcgggatg cacggacccg gcgtccggcg catgcggatg atccaccgcg
      241 ccggccccgg cggtcccggc gcgccgatgg ccatggagga ggtcggcggt cccggcgaca
      301 tgatgttcat gggcgaccct ggcgacgacg aggacatcga gatcgaactg ggcgagggtc
      361 ccgaggcgcc ggatcagtaa catccgcagc ttgcccgccg gtcgcccagc aggtaaccgg
      421 cgggcatgtt cgccttcctc gccaaccctg accggttcat ggccttctcg cgacgggccg
      481 cgccggcctt cggcgccctg gccgcggcgc tgatcgcggt cggcctgtgg ttcagcttcg
      541 ccgcgccgga ggactaccag cagggcgaca cggtgcggat catgttcatc catgtgccgg
      601 ccgcctatct ggcgatgttc agctacatct gcctgggcgt cgcgagcttc ctgtcgctgg
      661 tctaccgaca cgccctggcc gacgccgccg cccgggcctg cgctccgctg ggcgcggcct
      721 tcaccgccct ggccctggtc accggcgccc tgtggggcaa gccgatgtgg ggaacctggt
      781 gggtgtggga cgcgcgcctg acctcggtgc tggtgctgtt cctgttctgg ctcggctaca
      841 tggccctgcg cgcggcgatc gacgacgaaa ccaaggcggg ccgggcggcg gccatcctgg
      901 cgctggtcgg cctgatcaac ctgccggtga tccactattc ggtcgaatgg tggaacagcc
      961 tgcaccaggg tgcgacggtg ttcgccgcca aggccgagaa ccgcctcccg ccggcctatc
     1021 tcattcccat ggcgctgatg gcgctgggct acaccagcgc cttcgggtcg ctctggctgg
     1081 tgcggatccg tgcggaggtc tggcgccggc gggccgcagt gctggccctg caggcggcgg
     1141 aaggctgacg acgatgctcg atttcgatgc cggaaagtac gccgcctacg tctggccgtc
     1201 cttcatcata acggccgtgg ccttcggctg gatgatcgcc gacagcctgg cccgggcgcg
     1261 gcgatggaag cgcgaggtcg aggcccgcgg caaggaccgc ccatgaggcg ctggctggcc
     1321 ttcgtcccgc tggtggccct ggtcctgctc ggggtgctgt tcgccggcta ttcgttgaag
     1381 cgcaatccca acgtccagcc ggcggccctg gtcggccggc ccgtgcccga tctggccctg
     1441 ccggcgctgg agaccggccg gccgacgccg gttcgccagg ccgcgccagg gccgctgctg
     1501 atcaacttct acgcctcctg gtgcgccccc tgcgagcttg aggccccggc cctgatgcgg
     1561 ctcaggggcc agggggtgat catcctgggc atcggctaca aggatgcgcc ggagaacaat
     1621 cgggcctttc tcgggcgggt cggggatccg tacgccgcgc ggctgctcga ccgcgacggc
     1681 cgcgcgggtc tggagttcgg cgtcaccggc gtccccgaga gtttcctggt cgacgcctct
     1741 ggcgtgatca ccgccaagtt cactggcgaa ctgaccccgg ccgaagagcg cgagatcgtc
     1801 gccgccaccc ggcgttaacc gcgccttcac cacgccgccg catcttggcg tctcgttaac
     1861 catgtttccg agacgcccgt gactcccgtt cgacccaacg tctgggccaa tgtcccgccg
     1921 ccggcgcagg gctcggggca gggcgcggcc agatcggcgg cgcaacgggc ctttttcgac
     1981 gcggcgttgg gcaaggccgc caccaccacc ccggcccagc cccagacggc gccggcgaag
     2041 gccgtcccgc tccagaccca ggccccgtct gcgccgcagc cgctccagcg cacgccgtcg
     2101 gcccagcccg acgaaccgcc cgcccgcatc ctccgcccgg ggtcgctgct ggatatccgg
     2161 gtctgaccaa ccaccgtcat cctcgcgcct gtcgcgagga cccatgatct cggtggcgca
     2221 ggagcgaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 cgggcctggt cggcttgggc gccttggcgg ccttctcggg tttcgcgggc ttgtcggcct
     2461 tcggcggctt gatcttcttt tcccgggtcg gggcggcgac ggacgacagg gccgccagcg
     2521 cctcgaggaa ggccccgcgc ttgcccttgg gcagcagggc cagcaggcgg gcgtcggcgg
     2581 cgtcggcgcg cgggccgatt tccgccaggg cc
//
LOCUS       DAORNX010000418         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29857_length_2603_cov_8.227237, whole genome shotgun sequence.
ACCESSION   DAORNX010000418 DAORNX010000000
VERSION     DAORNX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29857_length_2603_cov_8.227237"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLO65_14590"
     CDS             <1..1396
                     /locus_tag="PLO65_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRD29525.1"
                     /translation="GEGRRMSRFSLRRAARAEPVAAPAAAPAPVVESPPPRAPHVSDE
                     QLDMRLRLHARLIEEIDLSKLGELDEGEMRRQVRRLVGDFARDSRLALNTAELDQMGE
                     EVFDEMVGLGPIEPLLKDESINDILINGPFQVYVERRGELELTPIRFRDNDHLLRIVN
                     RIVAAVGRRIDESSPMVDARLADGSRVNAAIAPIAIDGAAVSIRKFSKKPLSFERLVA
                     VGAMPACVAEFLHGAVRARVSTVISGGTGSGKTTLLNACSAAISHGERLITIEDAAEL
                     QLQQPHVVRMETRPPNIEGKGEIRQRELVKNALRMRPDRVILGEVRGEEAFDMLQAMN
                     TGHEGSMATIHSNSAREAITRLEQMVAMGGMTLSAEAVRGQIAAAVGMVVQVMRLSDG
                     KRKVMSVTEITGMEGNVVQMQDIFVFNRLRTEPDGTVIGEFRATGLRPKCLDEMIRKG
                     IPFDTAYFDPQRAL"
     gene            1527..2489
                     /locus_tag="PLO65_14595"
     CDS             1527..2489
                     /locus_tag="PLO65_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD29526.1"
                     /translation="MPDVDPRLVFMILIGAAVFAAAQAAWGLVRVAGTRRKVNKRLHL
                     AQTVTNLADLVVELRKQRGLDEHGNSRILVRWFSDLVIRSGVSYQPRTWTLYAAGLGL
                     IAYAMVFLWTKNPLLGLTAAPVFALGLPLLWLKIKAGARAKAIGLQLPNALEIIVRSL
                     EAGHPVPTAVALVGREMPDPLGSEFGMAGDEIAYGATLEQAVGRVADRCRHPDVDLFA
                     ATIRLQERSGGNLTGLLKMNAKAVRERHKMRLKIKAASGEGRASAIILTSAPFAVFAI
                     LQVASPHFYGDVIHERTVQLGLAALGTWMFIGNMVMRKMIDMRI"
BASE COUNT          413 a          903 c          894 g          393 t
ORIGIN      
        1 aggcgagggc cgtcggatga gccggttttc cctgcgtcgc gccgccaggg ccgagccggt
       61 ggccgccccc gccgcggccc ccgcgccggt cgtcgaaagc cccccgccga gggccccgca
      121 cgtctccgac gaacagctgg acatgcggct gcgactccat gcccggctga tcgaggaaat
      181 cgacctgtcc aagctcggcg agctggacga gggcgagatg cgtcggcagg tccggcggct
      241 ggtcggcgac ttcgcgcgcg acagtcggct ggcgctgaac accgccgaac tcgaccagat
      301 gggcgaggag gtgttcgacg aaatggtcgg cctggggccg atcgagcccc tgctcaagga
      361 cgagtcgatc aatgacatcc tgatcaacgg gccgttccag gtctacgtcg agcgccgcgg
      421 cgagctcgag ctgacgccga tccgcttccg cgacaacgat cacctgctgc gtatcgtcaa
      481 tcgcatcgtc gccgccgtcg gccggcgcat cgacgaatcg agcccgatgg tcgacgcacg
      541 cctggccgac ggcagccggg tcaacgcggc catcgccccg atcgccatcg acggggcggc
      601 ggtgtcgatc cgcaaattct ccaagaagcc gctgagcttc gagcggctgg tggcggtcgg
      661 ggccatgccg gcctgcgtcg ccgaattcct gcacggggcg gtccgggccc gcgtctcgac
      721 agtcatctcc ggcggcacgg gctcgggcaa gaccaccctg ctcaacgcct gttcggccgc
      781 catcagccac ggcgagcggc tgatcaccat cgaggatgcg gccgaactgc agctgcagca
      841 gccccacgtg gtgcgcatgg agacccgccc gccgaacatt gagggcaagg gcgagatccg
      901 ccagcgcgaa ctggtcaaga acgccctgcg catgcggccc gaccgggtga tcctgggcga
      961 ggtccgcggc gaggaagcct tcgacatgct gcaggccatg aacaccggcc acgagggctc
     1021 gatggccacg atccactcca actcggcccg cgaggccatc acccgcctgg agcagatggt
     1081 cgccatgggc ggcatgacgc tcagcgccga agccgtccgg ggccagatcg ccgccgccgt
     1141 cggcatggtg gtccaggtga tgcgcctgtc cgacggcaag cggaaggtga tgagcgtcac
     1201 cgagatcacc ggcatggaag gcaatgtcgt gcagatgcag gacatcttcg tcttcaaccg
     1261 gctgcggacc gagccggacg ggacggtgat cggcgagttc cgcgccaccg gcctgcgccc
     1321 caaatgcctc gacgaaatga tccgcaaggg catccccttc gacaccgcct acttcgaccc
     1381 gcagcgggcg ctgtgatggg tttgcgtcct tcgacacggc cctttgcgcc tgctcaggat
     1441 gacgtggggc ggggacgggt atccctccac gtcatgttga gcagcggccg gaggtcgcgt
     1501 gtcgaaccac gcagggagac gctctgatgc ccgacgtcga tcctcgcctg gtgttcatga
     1561 tcctgatcgg ggcggcggtg ttcgccgcgg cccaggccgc ctggggcctg gtccgggtcg
     1621 ccgggacccg gcgcaaggtc aacaagcggc tgcacctggc gcagacggtc accaacctgg
     1681 ccgatctggt ggtcgagctg cgcaagcagc ggggtctgga cgagcatggc aacagccgca
     1741 tcctggtgcg ctggttttcg gacctggtga tcagatccgg ggtcagctac cagccgcgca
     1801 cctggaccct ctacgccgcg ggcctgggcc tcatcgccta tgcgatggtg tttctctgga
     1861 cgaagaaccc gctgctgggc cttaccgccg cgcctgtgtt cgccctggga ctgccgctcc
     1921 tgtggttgaa gatcaaggcc ggcgcccggg ccaaggccat cggcctgcag ttgcccaacg
     1981 cgctcgagat catcgtccgc agcctcgagg ccggccaccc cgtgccgacc gccgtggccc
     2041 tcgtcgggcg cgagatgccc gacccgctcg ggagcgagtt cggcatggcc ggcgacgaga
     2101 tcgcctatgg cgccacgctc gagcaggcgg tcggccgggt cgccgaccgc tgccgccatc
     2161 cggacgtgga tctgttcgcc gcgaccatcc gactgcagga acggtcgggc ggcaacctga
     2221 ccggcctgct gaagatgaac gccaaggcgg tgcgcgaacg gcacaagatg cggctgaaga
     2281 tcaaggccgc ctccggcgag ggccgcgcct cggccatcat cctgacttcg gcgccgttcg
     2341 cggtgttcgc catcctgcag gtggccagcc cgcacttcta cggcgacgtg atccacgagc
     2401 ggaccgtcca gctcggcctg gcggccctcg ggacctggat gttcatcggc aacatggtca
     2461 tgcgcaagat gatcgacatg aggatctgag cccatggatc cgctgcagct gcaatccctg
     2521 ctgacctggc tggccatggc gctgatcgcg ctcggcgtcg gcggcgcggc cgtcctcggc
     2581 ctgcgcgaag tccgcgtcgg agc
//
LOCUS       DAORNX010000419         2599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_29930_length_2599_cov_6.575865, whole genome shotgun sequence.
ACCESSION   DAORNX010000419 DAORNX010000000
VERSION     DAORNX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29930_length_2599_cov_6.575865"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PLO65_14600"
     CDS             <1..105
                     /locus_tag="PLO65_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD29527.1"
                     /translation="PFGGVKESGLGREGSHFGLDEYLELKMMCIGLGG"
     gene            complement(181..257)
                     /locus_tag="PLO65_14605"
     tRNA            complement(181..257)
                     /locus_tag="PLO65_14605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Arg,seq:cct)
     gene            333..1634
                     /locus_tag="PLO65_14610"
     CDS             333..1634
                     /locus_tag="PLO65_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRD29528.1"
                     /translation="MKLLYVQVLIAIALGVAVGAIWPATGVALKPLADGFIKLIKLVI
                     APVIFVTVAGGIARMTDLKAFGRVGARTLVYFEVVSTLALAVGLLVGLVVRPGAGFDV
                     DPSTLDPTVAADYAAKAAHGEGTVAWLLHLIPDTFFGALANGELLQVLLIAILTGFAC
                     ARLGEFGDRVADTLDQIGRVFFGIIHIVVRLAPLGAFGAMGFTIGKYGVAALGNLAVL
                     IGTFYATSLIFVLVVLGLIARLAGFSILKFIGYIREELLIVLGTSSSESVLPQIMEKL
                     QRLGASRSVTGLVIPTGYSFNLDGTNIYMTLATLFLAQATNTPLDAWQLATILGVAML
                     TSKGASGVTGAGFITLAATLAVVPDIPIAALALIVGIDRFMSECRALTNLVGNGVATL
                     VVARWEGELDRETLARELARGPVAHDAHPLAAGLATEAEAD"
     gene            1732..2118
                     /locus_tag="PLO65_14615"
     CDS             1732..2118
                     /locus_tag="PLO65_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29529.1"
                     /translation="MARTLTRGLIGLVGLLALLLASRFWISPADIGAAMGLSADGPAG
                     LGTLRADMAGFFGASGVMFLLAAIRNDATWLRPLLLLLGIALAGRVINLLVTGGGQVL
                     IPPMVIEVVLIAVTLLGMRVLGARKG"
     gene            complement(2254..>2599)
                     /locus_tag="PLO65_14620"
     CDS             complement(2254..>2599)
                     /locus_tag="PLO65_14620"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRD29530.1"
                     /translation="DRSLDAPIAGDGEEDWLGLLPDERDRPDQQVQAILDGERRTGLV
                     GRSMAVLTARERSVIEARRLAEDPVTLEALGARMGVSKERVRQIEQAALGKLRAFIER
                     EVGDPVAAGLIG"
BASE COUNT          333 a          918 c          927 g          421 t
ORIGIN      
        1 ccgttcggcg gcgtcaagga atcgggcctc ggccgcgagg gctcccactt cggcctcgac
       61 gagtacctcg aactgaagat gatgtgcatc ggcctgggcg gctgaccgcc gttgcgatga
      121 cgcacatcac ccgatcccgg gtgaacaacc gtctccgcac cgccagccag tccgactgtc
      181 tggcggcccg ggagggactc gaacccccga ccttcgcttt aggaaagcgc tgctctatcc
      241 tgctgagcta ccgggccacg cggcccggtc atagcgtgcg gggagcctca gcggaacccc
      301 ggcggcggcc tgccgggggg tgaatgcggt atctgaagct gctctatgtc caggtgctga
      361 tcgccatcgc gctcggcgtc gccgtgggcg cgatctggcc ggccaccggc gtggcgctca
      421 agcctctggc cgacggcttc atcaagctga tcaagctggt catcgcgccg gtgatcttcg
      481 tcaccgtcgc cggcggcatc gcccggatga ccgacctgaa ggcgttcggc cgggtcgggg
      541 ccaggacgct ggtctatttc gaggtggtct cgaccctggc cctggcggtc gggctgctgg
      601 tcgggctggt ggtgcgcccg ggggcgggct tcgacgtcga cccgtcgacc ctcgatccga
      661 cggtggcggc cgactacgcg gccaaggcgg cgcacgggga ggggaccgtc gcctggctgc
      721 tgcacctgat ccccgacacc ttcttcggcg ccctggccaa tggcgagctg ctgcaggtgc
      781 tgctgatcgc catcctgacc ggcttcgcct gcgcccggct gggcgagttc ggcgacaggg
      841 tggccgacac gctggatcag atcggccggg tgttcttcgg catcatccac atcgtggtgc
      901 ggctggcgcc gctgggcgcc ttcggggcca tgggcttcac aatcggcaag tacggcgtgg
      961 cggcgctggg caacctggcg gtgctgatcg ggaccttcta cgccaccagc ctgatcttcg
     1021 tgctggtcgt gctgggcctg atcgcccgcc tggccggctt ctcgatcctg aagttcatcg
     1081 gctacatccg cgaggagctg ctgatcgtgc tgggcaccag ctcgtcggag tcggtgctgc
     1141 cgcagatcat ggagaagctg cagcgactgg gcgcctcgcg gtcggtgacc ggcctggtga
     1201 tcccgaccgg ctacagcttc aacctcgacg gcaccaacat ctacatgacc ctggccaccc
     1261 tgttcctggc ccaggcgacc aacacccccc tcgacgcctg gcagctggcg accatcctgg
     1321 gcgtggccat gctgacctcc aagggcgcca gcggcgtgac cggcgccggc ttcatcaccc
     1381 tggccgcgac cctggccgtg gtccccgaca tcccgatcgc cgccctggcc ctgatcgtcg
     1441 gcatcgaccg gttcatgagt gaatgccggg ccctgaccaa tctggtcggc aacggggtgg
     1501 cgaccctggt ggtcgcccgc tgggaaggcg agctcgaccg cgagaccctg gcgcgggagc
     1561 tggcgcgcgg cccggtcgcc cacgacgccc atccgctggc cgcggggctg gcgaccgagg
     1621 cggaggccga ctgacgaatc cggttaccgg gtggtaatcg cgcgcgggcg tgctagcctc
     1681 gcggccggcg cgtccgcggg agacgcggaa cacgcaaccg gggaggggcc catggcccgg
     1741 acgttgacgc ggggcctgat cggcctggtg ggcctgctgg cactgttgct ggccagccgc
     1801 ttctggatca gcccggcgga catcggcgcg gccatgggcc tgtcggccga cggaccggcc
     1861 ggactgggaa ccctgcgggc cgacatggcc ggtttcttcg gggcttccgg cgtgatgttc
     1921 ctgctggccg cgatccgcaa cgacgcgacg tggctgcggc cgctgctgct gctgctgggg
     1981 atcgcgctgg cgggccgggt gatcaacctg ctggtcaccg gcggcggtca ggtcctgatc
     2041 ccgccgatgg tcatcgaggt cgtgctgatc gcggtgaccc tgctgggaat gcgggtgctc
     2101 ggcgcccgga aggggtaggg cagcgccacg ttgtcttcgt ggcggacctc gccctgatga
     2161 cttgacggcc aggattgctc cggcgggtcc ccgctcaagg gccgcctgcg gcagccgcga
     2221 agcggcggcc ccgcctttgg aaatccggcc cccctagccg atcaggcccg ccgccaccgg
     2281 atcgccgacc tcgcgctcga tgaaggcgcg cagcttgccc agggcggcct gttcgatctg
     2341 gcgcacgcgt tccttggaca cccccatccg ggcgcccagg gcctcgagcg tgaccggatc
     2401 ctcggccagc cggcgggcct cgatcaccga ccgctcacgg gcggtcagca ccgccatcga
     2461 ccggccgacc aggccggtgc ggcgctcgcc gtcgaggatc gcctggacct gctggtcggg
     2521 ccggtcgcgc tcgtcgggca gcaggcccag ccagtcctcc tcgccgtcgc cggcgatcgg
     2581 cgcgtcaagc gaccggtcc
//
LOCUS       DAORNX010000420         2585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30150_length_2585_cov_9.823715, whole genome shotgun sequence.
ACCESSION   DAORNX010000420 DAORNX010000000
VERSION     DAORNX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30150_length_2585_cov_9.823715"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLO65_14625"
     CDS             <1..318
                     /locus_tag="PLO65_14625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRD29531.1"
                     /translation="KFGSDLDAATQRQLARGERLTELLKQPQYAPLAVEEQVCVIYAG
                     TRGYLDGIPTAAVGRFEREFLARLHSKHQDLLDAIRTTKALGADLEDTLKKALESFTA
                     TFA"
     gene            351..1226
                     /locus_tag="PLO65_14630"
     CDS             351..1226
                     /locus_tag="PLO65_14630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRD29532.1"
                     /translation="MASLKDMRNRISSVKATQKITKAMQMVAAAKLRRSQDAAQNARP
                     YAERMAAVIANLAAGVSGDSAPRLLAGTGADQRHLIVVAAADRGLAGGFTSSIVRAAR
                     DRIAALLAEGKDVKIVTVGKKALPPLRRLYGDRIVQSFDLSAERTLTLSVAQPIADRI
                     IELYEAGDVDVVTLFYSRFKSVVSQTPTARQLIPAQVEGGGVTDLQGAVYEYEPSEEG
                     ILETLLPRNITVQVLSALLENMAGFYASQMTAMDNATRNAGDMIKGLTVQYNRTRQAQ
                     ITKELIEIISGAEAL"
     gene            1258..>2585
                     /gene="atpD"
                     /locus_tag="PLO65_14635"
     CDS             1258..>2585
                     /gene="atpD"
                     /locus_tag="PLO65_14635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRD29533.1"
                     /translation="MATAPKKAAAPKAAAPKAPAKKAAPKAAAAQTAAPKAVKPSAVV
                     ATGKIVQVIGAVVDVEFDGHLPAIMNALETTNLDQKTGEPFRLVLEVAQHLGENTVRT
                     IAMDTTEGLTRGQDVADTGKGIMVPVGPATLGRIMNVIGEPIDEAGPIVTPHYNVIHR
                     EAPSFAEQSTSAEVLVTGIKVIDLMCPYTKGGKIGLFGGAGVGKTVTMQELINNIAKA
                     YGGYSVLAGVGERTREGNDLYHEMIESNVNVDPKHNNGSTEGSKCALVYGQMNEPPGA
                     RARVALTGLTQAEYFRDVEGKDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLA
                     TEMGNLQERITSTNKGSITSVQAIYVPADDLTDPAPAASFAHLDATTVLSRDIAAQAI
                     FPAVDPLDSTSRIMDPLVIGEEHYRVARQTQEILQQYKQLKDIIAILGMD"
BASE COUNT          485 a          961 c          797 g          342 t
ORIGIN      
        1 aagttcggct ccgacctcga cgccgccacc cagcgccagc tggcccgcgg cgagcgactg
       61 accgagctgc tcaagcagcc gcagtacgcg ccgctggcgg tcgaggagca ggtgtgcgtg
      121 atctacgccg gcacccgcgg ctatctcgac ggcatcccga ccgccgccgt cggccgcttc
      181 gagcgcgagt tcctggcccg cctgcacagc aagcaccagg acctgctgga cgcgatccgc
      241 accaccaagg ccctcggcgc cgatcttgag gacaccctca agaaggccct cgagagcttc
      301 accgcgacct tcgcgtaaga ctggccgaga gcgagagaga gtagccctcg atggccagcc
      361 tcaaggacat gcgcaatcgg atctcgagcg tgaaagccac gcagaagatc acgaaggcca
      421 tgcagatggt cgccgccgcc aagctgcgcc gcagccagga cgccgcgcag aacgcgcgtc
      481 cctatgcgga acggatggcc gcggtgatcg ccaatctcgc cgccggcgtc agcggcgaca
      541 gcgccccgcg gctgctcgcc ggcacgggcg ccgatcagcg tcacctcatc gtcgtggccg
      601 ccgccgaccg cggcctggcg ggcggcttca cctcgtccat cgtccgcgcg gcgcgtgatc
      661 gcatcgccgc cctgctggcc gagggcaagg acgtgaagat cgtcaccgtc ggcaagaagg
      721 ccctgccgcc gctgcgccgc ctgtacggcg accggatcgt ccagtcgttc gacctgtcgg
      781 ccgagcgcac cctgaccctg tcggtggccc agccgatcgc cgatcgcatc atcgagctct
      841 acgaagccgg cgatgtcgac gtggtcaccc tgttctacag ccgcttcaag tcggtggtca
      901 gccagacccc gaccgcccgg cagctgatcc cggcccaggt cgagggcggc ggcgtcaccg
      961 acctgcaggg cgccgtctac gaatacgaac cctccgaaga gggcatcctc gagaccctgt
     1021 tgccgcgcaa catcacggtg caggtgctct ccgccctgct cgagaacatg gccggcttct
     1081 acgccagcca gatgacggcc atggataacg ccacgcgcaa cgccggcgac atgatcaagg
     1141 gcctcactgt ccaatacaac cgcacccgcc aggcgcagat caccaaggag ctgatcgaga
     1201 tcatctccgg cgccgaagct ctctgaccgc ataaagaccg cacggaagac cagaccaatg
     1261 gccaccgccc ccaagaaagc cgccgctccc aaggccgccg cgcccaaggc gccggcgaag
     1321 aaggctgctc cgaaagccgc cgccgcccag accgccgccc ccaaggccgt caagccgtcc
     1381 gccgtcgtgg cgaccggcaa gatcgtccag gtcatcggcg ccgtggtcga cgtggagttc
     1441 gacggccacc tgccggcgat catgaacgcg ctcgagacca ccaacctcga ccagaagacc
     1501 ggggagccct tccgcctggt gctcgaggtc gcccagcacc tgggcgagaa caccgtccgc
     1561 accatcgcca tggacaccac cgagggcctg acccgcggcc aggacgtcgc cgacaccggc
     1621 aagggcatca tggtgccggt cggcccggcg accctgggcc gcatcatgaa cgtcattggc
     1681 gagccgatcg acgaggccgg tccgatcgtc acgccgcact acaacgtcat tcaccgcgag
     1741 gccccgagct tcgcggagca gtcgacctcg gccgaagtgc tggtcaccgg catcaaggtc
     1801 atcgacctga tgtgccccta caccaagggc ggcaagatcg gcctgttcgg cggcgccggc
     1861 gtgggcaaga ccgtgaccat gcaggagctg atcaacaaca tcgccaaggc ctacggcggc
     1921 tattcggtgc tggccggcgt gggcgagcgc acccgcgagg gcaacgatct ctatcacgag
     1981 atgatcgagt ccaacgtgaa cgtggacccc aagcacaaca acggctcgac cgaaggctcc
     2041 aagtgcgccc tggtctacgg ccagatgaac gagcccccgg gcgcccgcgc ccgcgtcgcc
     2101 ctgaccggcc tgacccaggc cgagtacttc cgcgacgtgg aaggcaagga cgtgctgctg
     2161 ttcatcgaca acatcttccg cttcacccag gccggttcgg aagtgtcggc gctgctgggc
     2221 cgcatcccct cggcggtggg ctatcagccc accctggcca cggagatggg caacctgcag
     2281 gagcgcatca cctcgaccaa caaggggtcg atcacctcgg tgcaggccat ctacgtgccc
     2341 gccgacgacc tgaccgaccc ggccccggcc gcctcgttcg cccacctcga cgccacgacc
     2401 gtgctgagcc gtgacatcgc cgcccaggcc atcttcccgg ccgttgaccc gctggactcg
     2461 accagccgga tcatggatcc cctggtgatc ggcgaggaac attaccgcgt cgcccgtcag
     2521 acccaggaga tcctgcagca gtacaagcag ctgaaggaca tcatcgccat cctgggcatg
     2581 gacga
//
LOCUS       DAORNX010000421         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30232_length_2579_cov_8.109746, whole genome shotgun sequence.
ACCESSION   DAORNX010000421 DAORNX010000000
VERSION     DAORNX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30232_length_2579_cov_8.109746"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLO65_14640"
     CDS             complement(<1..290)
                     /locus_tag="PLO65_14640"
                     /inference="COORDINATES: protein motif:HMM:NF023070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29534.1"
                     /translation="MANVMITYRLRAGVDRAAFETWVRDYDYPNIRGVRRVTAFTNHR
                     VERLLIGEGAPSVDYVEVFDLDDLDGFIAEDLTGPVIQTVMGAFMAWVEEPQ"
     gene            446..1483
                     /locus_tag="PLO65_14645"
     CDS             446..1483
                     /locus_tag="PLO65_14645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRD29535.1"
                     /translation="MGYRVAVVGATGNVGREMFNILEEVNFPVDRIHAIASRKSIGVE
                     VSFGEKILKCEDLEQFDFSTVDIVLMSAGGEVSRAWAERIGKAGPVVIDNSSSWRMDP
                     DVPLIVPEVNPDAVEGFTKKNIIANPNCTTAGLLVALKPLHDAAKIRRVVISTYQSVS
                     GSGKEGMDELWEQTKGVFVLGAPEPKKYPKQIAFNVIPQCDSFIDDDSGDTKEERKMV
                     DETHKMLDPAIKVSATCVRVPVFVGHGEAVNVEFERPISDDEAREILREAPGVVVIDK
                     REDGGYATPKDIQGEFPVYVSRIRNDPTLEHGLSLWVVSDNLRKGAALNAVQIAQLLH
                     DRGLIKQRALA"
     gene            1564..2463
                     /gene="rarD"
                     /locus_tag="PLO65_14650"
     CDS             1564..2463
                     /gene="rarD"
                     /locus_tag="PLO65_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRD29536.1"
                     /translation="MPPAKDEGRAALAAGLACYTIWGLFPIYMHALGAAGVSPLEITA
                     ERTAWALLWAAGLVLAARKTTELKRVLGEPRTLGLLLGSSLLIGVNWLLYVIAVNSGR
                     TLEASLGYYINPLMNMAAGALFFRERIDRIGLSAIALAAIGVAVQTAAVGHPPLLSLG
                     LAISFCGYGLIRKQVRADAQTGLLVETAILSLPAIAYVVWLQKTGVGHLGDGVWTTVL
                     LLCAGPLTVAPLALFAWAARRLPLVALGFLQFIAPTLQFFVGVASGEAFTPMRALAFG
                     FIWIGAGVFAYGAWRRTRRLAIA"
BASE COUNT          404 a          852 c          889 g          434 t
ORIGIN      
        1 aactggggct cttcgaccca ggccatgaac gcgcccatca ccgtctggat caccggcccg
       61 gtgagatcct ccgcgatgaa gccgtcgagg tcgtcgaggt cgaacacctc gacatagtcg
      121 acagagggcg cgccctcgcc gatcagcagc cgctcgaccc ggtggttggt gaaggccgtg
      181 acgcgccgga cgccgcggat gttggggtag tcgtagtcgc gcacccaggt ctcgaacgcc
      241 gcccggtcca cgccggcccg tagcctgtag gtgatcatca cgttggccat gacgccctcc
      301 gttgtcctgt tgtgcagacc tagtgcgcaa ggcgtcgaga accagacccg aaaggggggc
      361 tgacggcggc ttgagctttc ctccgcttgc gccgtcaggt tcgggggcgt aacgggaacg
      421 cgcttttcag cgaaggagac aagcgatggg ctaccgggtc gccgtggtcg gcgccacggg
      481 caatgtgggc cgtgagatgt tcaacatcct cgaggaagtg aatttccccg tggaccggat
      541 tcacgccatc gccagccgca agtccatcgg tgtcgaagtg tccttcggcg aaaagatcct
      601 caaatgcgag gacctggagc agttcgactt ctcgaccgtc gacatcgtgc tgatgagcgc
      661 gggcggcgaa gtgtccaggg cctgggccga gcggatcggc aaggccggcc cggtggtgat
      721 cgacaactcc tcgtcctggc gcatggatcc ggacgtgccg ttgatcgtgc cggaggtgaa
      781 tccggacgcg gtcgagggct tcacgaagaa gaacatcatc gccaacccca actgcaccac
      841 ggccggcctg ctggtggcgc tcaagccgct gcatgacgcc gcgaagatca ggcgtgtcgt
      901 gatctcgacc tatcagtcgg tgtccggctc gggcaaggaa ggcatggacg agctgtggga
      961 gcagaccaag ggcgtgttcg tcctcggcgc gcccgagccg aagaaatacc ccaagcagat
     1021 cgccttcaac gtgatcccgc agtgcgactc gttcatcgac gacgactccg gcgacaccaa
     1081 ggaggagcgc aagatggtcg acgagacgca caagatgctc gatccggcga tcaaggtcag
     1141 cgccacctgc gtacgcgtgc cggtgttcgt cggccatggc gaggcggtca acgtcgagtt
     1201 cgagcggccg atcagcgacg acgaggcccg cgagatcctc cgcgaggcgc cgggcgtggt
     1261 ggtcatcgac aagcgcgagg acggcggcta cgccacgccc aaggacatcc agggcgagtt
     1321 cccggtctat gtcagccgca tccgcaacga cccgaccctc gagcacggcc tgtcgctgtg
     1381 ggtggtctcc gacaacctgc gcaagggcgc ggcgctcaac gccgtgcaga tcgcccagct
     1441 gctgcatgac cgcgggctga tcaagcagcg cgccctggcc tgacgaacgg cccgcaagat
     1501 gcgggtggat cattcagggg cccgggacca gtttccgggc cccttctctt tgggagtttc
     1561 gccatgccgc cggcgaagga cgaaggccgc gccgccctgg ccgctggcct ggcctgctac
     1621 accatctggg gcctgttccc gatctatatg cacgccctcg gcgcggccgg ggtctcgccg
     1681 ctggagatca ccgccgaacg aaccgcctgg gcgctgctgt gggccgccgg gctggtgctg
     1741 gcggcgcgca agacgaccga gctgaagcgg gttctcggcg agccccggac cctcggcctg
     1801 ttgctcggct catcgctgct gatcggcgtc aactggctgc tgtacgtcat cgccgtgaac
     1861 agcggccgga cgctcgaggc cagcctcggc tactacatca atccgctgat gaacatggcg
     1921 gcgggcgccc tgttcttccg tgaacggatc gaccggatcg gcctgtcggc catcgccctg
     1981 gcggccatcg gcgtggcggt gcagaccgcc gccgtcggcc acccgccgct gctctcgctg
     2041 ggcctggcga tcagcttctg cggctatggc ctgatccgca aacaggtgcg ggccgacgcc
     2101 cagaccggcc tgttggtcga gaccgccatt ctgtcgctcc cggccatcgc ctatgtcgtc
     2161 tggcttcaga agaccggcgt cggtcatctg ggcgacggcg tctggaccac cgtgctgctg
     2221 ctctgcgccg ggccgctgac cgtcgcgccc ctggcgctgt tcgcctgggc ggcccggcgg
     2281 ctgccgctcg tggccctggg cttcctgcag ttcatcgccc cgacgctgca gttcttcgtc
     2341 ggcgtcgcgt cgggcgaggc gttcacgccg atgcgggccc tggcgttcgg cttcatctgg
     2401 atcggcgccg gggtgttcgc ctacggcgcc tggcggcgca cccgacggtt ggcgatcgcc
     2461 tgaaacgggg tagggtcggc cggtgatgac ccggcggaca gtcctgacga gcggcgcggc
     2521 gatcgcgatg acggcggcgg gcttcggatt gccgttcgga ttgtattacg gatggcgga
//
LOCUS       DAORNX010000422         2567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30420_length_2567_cov_5.613854, whole genome shotgun sequence.
ACCESSION   DAORNX010000422 DAORNX010000000
VERSION     DAORNX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30420_length_2567_cov_5.613854"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLO65_14655"
     CDS             <1..956
                     /locus_tag="PLO65_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD29537.1"
                     /translation="STAAGRHHATMQALPRLWLEFLAVTGLAALVLLARPPAGDPAGL
                     VPLLALFAAAAFRLIPSANRIINGYQSLRYFGPTIGSVAVELALATPPAPETSGPLPP
                     FERELLVDQVTYGYPGASDPVLDAVTVRIGRGSAVGFAGPSGAGKSTLLDIILGLLTP
                     DSGQVCVDGAPITDNLRDWRGRIGYVPQTIQLADDTIRRNVAFGQADHAISEAAVRAA
                     LRMARLEDYVASLPDGLDTVIGEHGARMSGGQRQRLGIARALYGKPSLLVLDEPTSAL
                     DPLNEQAIIETLLALRPELTVLIASHRTAALEGCDHVVQMG"
     gene            complement(944..2041)
                     /locus_tag="PLO65_14660"
     CDS             complement(944..2041)
                     /locus_tag="PLO65_14660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRD29538.1"
                     /translation="MYLLADTAAEKSAGADLTAGGIEPGLVSVIVPTLNRARLVTELV
                     EALFAQTWRPVEIIVVDDGSSDDTLARLTTMTPPDADCSITILRQENAGPAAARNHGL
                     RQARGEFVYFVDSDDLVFPLALETLVRPLRSSDAPFALAEIRNADEAGALLPDDGAGR
                     PRLREGDVFASHWMTHNALYRRSAIDAAGGFNETLRCGEDNEFQWRVMTVSGRPHVTT
                     TLIGVRRLHGYGHLSVGRPIEERSRWTVDAFTAFQAWALSRGDQAAIPRRFRWRLLAC
                     GIRLGSVADWIGKDRAFAAIAGFPARGLDPAWMVWLGRHRNRALFAMLRAGGEALKAA
                     RDALAGRPAGSTVEAEARRDAGQPGGGTHPI"
     gene            2231..>2567
                     /locus_tag="PLO65_14665"
     CDS             2231..>2567
                     /locus_tag="PLO65_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRD29539.1"
                     /translation="MTTRREVVRQGAALFGLAAASPVAAAAGAAESPDQTVAALLDRQ
                     AKALLRDSPEMATRLGFDTGPEAFLRSRLDDRSADAMTARGARAAAAAAALGSIDRTG
                     LSAQAALDLD"
BASE COUNT          384 a          941 c          883 g          359 t
ORIGIN      
        1 cgagcacggc ggccggccgg caccacgcca cgatgcaggc cctgccgcgc ctgtggctgg
       61 agttcctcgc cgtgacgggc ctggcggcgc tggtcctgct ggcgcggccg ccggccggcg
      121 accccgccgg actcgtgccg ttgctggcgc tgttcgccgc cgccgccttc cggctgattc
      181 cgtcagccaa ccgcatcatc aatggctatc agagcctgcg ctatttcgga ccgacgatcg
      241 gcagcgtggc ggtggaactg gcgctggcga caccgccggc gccggagacc tccggaccgc
      301 tgcccccgtt cgaacgagag cttctcgtcg accaggtcac ctatggctat cccggcgcct
      361 cggacccggt gctcgatgcg gtcaccgtcc gcatcgggcg cggcagcgcc gtcggcttcg
      421 ccgggccgag cggagccggc aagagcacgc tgctggacat catccttggc ctgctgacgc
      481 cggactccgg ccaggtgtgc gtcgacggcg cgccgatcac cgacaacctg cgcgactggc
      541 gcggtcgcat cggctacgtg ccgcagacca ttcagctggc cgacgacacg atccgccgca
      601 acgtcgcctt tggccaggcc gaccacgcca tctcggaggc ggcggtcagg gcggccctgc
      661 gcatggcccg cctcgaggat tatgtcgcga gcctgccgga cggcctggac acggtgatcg
      721 gcgagcacgg cgccaggatg tcggggggac aacgccagcg gctgggcatc gcccgcgcgc
      781 tttacggaaa gccctcgctg ctggtgctcg acgagcccac cagcgcgctc gaccccttga
      841 acgagcaggc gatcatcgag accctgctgg ccctccgtcc ggaactgacc gtgctgatcg
      901 cctcccacag gaccgcggcc ctcgagggat gcgaccacgt tgttcagatg gggtgagtcc
      961 caccaccggg ttggcccgcg tcgcgccggg cctccgcctc gaccgtcgac cccgccgggc
     1021 ggccagccag ggcgtcacgc gcggccttga gcgcctcgcc gcccgccctc agcatcgcga
     1081 agagggccct gttgcggtgg cggcccagcc agaccatcca ggccggatcg agcccgcggg
     1141 ccggaaatcc ggcgatcgcc gcgaacgccc ggtccttgcc gatccagtcg gccacactgc
     1201 cgaggcggat cccgcaggcc agcaatcgcc accggaagcg gcgtggaatg gccgcttggt
     1261 cgccccggga cagggcccag gcctggaacg cggtgaaggc gtccacggtc catcggctgc
     1321 gctcctcgat cggccgcccg acgctgagat gcccgtagcc gtggaggcgg cgaacgccga
     1381 tgagggtcgt ggtgacgtgc ggcctgccgc tgaccgtcat gacgcgccat tggaactcat
     1441 tgtcttcccc gcaacgcagg gtttcgttga agccccccgc ggcatcgatg gccgaccggc
     1501 gataaagggc gttgtgcgtc atccagtggc tcgcgaagac gtcaccctcc cgcagccgcg
     1561 gcctgccagc tccatcgtcc ggcaacagcg cgccggcctc gtcggcgttg cggatttccg
     1621 caagggcaaa gggcgcgtcg ctcgaccgca gcgggcgaac cagcgtctcg agcgcgaggg
     1681 ggaagaccag gtcgtcggaa tcgacgaaat agacgaactc accccgggcc tgcctcagcc
     1741 catggttccg ggcggccgcc ggtccagcgt tctcctgccg caggatcgtg atcgaacagt
     1801 cagcgtcagg cggcgtcatg gtcgtcagac gcgcgagcgt atcgtcgctg gagccatcgt
     1861 cgacgacgat gatctcgacc ggacgccatg tctgcgcaaa cagcgcctcg accagctcgg
     1921 tcaccagccg cgcgcggttc aaggtcggca cgatgaccga gaccagaccc ggttcgatcc
     1981 cgccggcggt cagatcggcg cccgccgact tctccgccgc tgtgtcggcc agaagataca
     2041 tgtccgcgtc gcttgagccg caccccgccc ccggagccgg gagcatgatg aaggattgtc
     2101 ctgcaaaggc aaccggaccc ggacgcgctc gccgattgca accgttccgg gccaaggccc
     2161 gcggttcggc gttgaaaacg ggcggtcgcc agatcaaacc tgacctccac gactcacgag
     2221 gaccgcagca gtgaccacca gacgcgaagt cgtgcgacag ggcgcggcgc tgttcgggct
     2281 tgcggcggct tcgcctgtcg ccgccgccgc gggcgctgcg gaatcgccgg accagacggt
     2341 ggcggcgctg ctggaccgac aggcgaaggc cctgctgcgg gattctccgg aaatggcgac
     2401 ccggctgggg ttcgacaccg gcccggaggc cttcttgcga tcgcggctcg acgaccggtc
     2461 cgctgacgcc atgacggccc ggggcgcccg cgccgccgcc gccgcagccg cgctgggcag
     2521 cattgaccgc acgggcctct cggcgcaggc ggcgctggac ctggacg
//
LOCUS       DAORNX010000423         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30650_length_2552_cov_8.801762, whole genome shotgun sequence.
ACCESSION   DAORNX010000423 DAORNX010000000
VERSION     DAORNX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30650_length_2552_cov_8.801762"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLO65_14670"
     CDS             complement(<1..536)
                     /locus_tag="PLO65_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD29540.1"
                     /translation="MNQEDYRAQDATGLAALIADKQVSADEVLDAAIARMEQVNPTLN
                     AVILDLTDQARAQLKQGTPAGPLGGVPWLIKDIGALVAGTPTTSGSRLLKDAVAPADS
                     AIVTAYRKAGLVIFGKTNTPEFGLEPVTEPEFFGPTLNPWDLDRTPGGSSGGASAAVA
                     GGIVPAAHASDGGGSIRTP"
     gene            705..2186
                     /locus_tag="PLO65_14675"
     CDS             705..2186
                     /locus_tag="PLO65_14675"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD29541.1"
                     /translation="MATEHFDVVIVGAGISGVGAGYHLQDKSPDRSYVILEGRDSMGG
                     TWDLFRYPGIRSDSDMYTLGYSFRPWTDAKAIADGPAILKYVKETAAHYGIDRHIRYG
                     CMVKRARWSTETATWTVEVEKAGQAVEFTCNFLFLCGGYYSYVEGYTPDWAGMETFKG
                     DIVHPQKWPEGLDYSGKKVVVIGSGATAVTLVPEMAKAAGHVTMLQRSPTYVVSRPAE
                     DSIANWLRGKLPAMTAYGLTRWKNVLLNMVFYNLARKNPGKTRERLIGMVRDQLGPDY
                     DVATHFTPTYNPWDQRLCLVPDADLFDAIKAGKASVVTDHIETFTPKGLKLKSGAELE
                     ADVIVTATGLNLQVFNGLELVVDGQVVEPAKTMSYKGMMYSDVPNLASSFGYTNASWT
                     LKCDLTCEYVTRLLNHMSRTGQRQCTPRRTDPTIQEEPWLDFSSGYVQRSIGRFPRQG
                     SKTPWKLHQNYARDLMALKFGSVTDGVMEFSNPAPKDRLRAAA"
     gene            complement(2197..>2552)
                     /locus_tag="PLO65_14680"
     CDS             complement(2197..>2552)
                     /locus_tag="PLO65_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRD29542.1"
                     /translation="DGFIFLVDRKKDMVLRGGENIYCAEVEATIHQHPAVAECCVFGV
                     PDARLGEEVGAAIVLRAGETLSANDIREHCISLVARHKAPRYVWLLDQSLPRNANGKF
                     LKRELRESLKVEDAA"
BASE COUNT          436 a          872 c          842 g          402 t
ORIGIN      
        1 ggggtgcgga tcgagccgcc gccatcgctg gcgtgggccg cgggaacgat gccgccggcg
       61 accgccgcac tggccccgcc cgaggagccg cccggggtgc ggtcgagatc ccagggattg
      121 agcgtcggtc cgaagaattc cggttcggtg acgggctcca ggccgaactc cggcgtgttg
      181 gtcttgccga agatcaccag cccggccttg cgataggcgg tcacgatagc gctgtcggcc
      241 ggcgcgacgg cgtccttgag caggcgcgaa ccgctggtcg tcggcgtccc ggccaccagg
      301 gcgccgatat ccttgatcag ccagggcacg ccgcccagcg ggccggcggg ggttccctgt
      361 ttcagctgcg ccctggcctg gtcggtcaga tcgaggatta ccgcgttcag cgtcggattg
      421 acctgctcca tccgggcgat ggcggcgtcg agcacctcgt ccgcgctgac ctgcttgtcg
      481 gcgatcaggg ccgccagccc ggtggcgtcc tgcgcccggt agtcttcctg gttcatcgcc
      541 gtctcccgcg gactcgttga tgcgatccga ccacagaccg gccgcgtcgc caatgccgcc
      601 cggccgtcaa agttaacact gatcacttta tcggagacaa accggcccgt gagaggcaca
      661 atcgcgccgg ggaatgactc tcaatctcag aagcggggcg cggcatggcg accgaacatt
      721 tcgacgtggt gatcgtcggc gccgggatct ccggcgtcgg ggcgggctac catctgcagg
      781 acaagtcgcc cgaccgcagc tacgtcatcc tcgagggccg cgacagcatg ggcggcacct
      841 gggacctgtt ccgctatccg ggcatccgct ccgactccga catgtacacc ctgggctatt
      901 ccttccgccc ctggacggac gccaaggcca tcgccgacgg cccggcgata ctgaagtacg
      961 tcaaggaaac cgcggcccac tatggcatcg acaggcacat ccgctacggc tgcatggtca
     1021 agcgagcccg ctggtcgacc gaaacggcga cctggaccgt cgaggtggag aaggccggcc
     1081 aggcggtgga attcacctgc aacttcctgt tcctgtgcgg cggctactac agctacgtcg
     1141 agggctacac ccccgactgg gccgggatgg agacgttcaa gggcgacatc gtccatccgc
     1201 agaagtggcc cgaagggctc gactacagcg gcaagaaggt ggtggtcatc ggcagcggcg
     1261 cgacggcggt caccctcgtt ccggagatgg ccaaggccgc cggccacgtg accatgctgc
     1321 agcgctcgcc gacctatgtg gtctcgcggc cggccgagga ttccatcgcc aactggctgc
     1381 gcggcaagct gccggcgatg accgcgtacg gcctcacgcg ctggaagaac gtgctgctca
     1441 acatggtgtt ctacaatctg gcgcggaaga atccgggcaa gaccagggag cggctgatcg
     1501 ggatggtcag ggaccagctg ggccccgact acgacgtcgc cacccacttc accccgacct
     1561 acaatccctg ggaccaacgg ctgtgcctgg tgcccgacgc cgacctgttc gacgccatca
     1621 aggcgggcaa ggcctcggtg gtcaccgatc acatcgagac cttcaccccg aaaggcctga
     1681 agctgaagtc cggcgccgag ctggaggccg acgtcatcgt cacggccacc ggcctgaacc
     1741 tgcaggtgtt caacggcctg gaactggtcg tcgacggcca ggtcgtcgag cccgcgaaga
     1801 ccatgagcta caagggcatg atgtacagcg acgtgcccaa cctggcctcg tcgttcggct
     1861 acaccaacgc gtcctggacg ctgaagtgcg acctgacctg cgagtatgtc acccgcctgc
     1921 tcaaccacat gagccgcacg ggccagcgcc agtgcacgcc gcgccgcacc gacccgacga
     1981 tccaggaaga gccgtggctc gacttctcct caggctacgt ccagcgctcg atcgggcgct
     2041 tcccccggca gggttcgaag accccgtgga agctgcacca gaactacgcc cgcgacctga
     2101 tggcgctgaa attcggctcg gtgacggacg gggtgatgga gttctccaac cccgcgccga
     2161 aggaccgttt gagggcggcc gcctgaggcc cgcggctcag gccgcgtcct cgaccttcag
     2221 gctttcccgc aactcacgct tgaggaactt gccgttggcg ttgcggggca gcgactggtc
     2281 cagcagccag acgtaacgcg gcgccttgtg gcgcgcgaca aggctgatgc agtgctcgcg
     2341 gatgtcgttg gccgacaggg tctcgcccgc gcgcagtacg atggccgcgc cgacctcctc
     2401 gcccaggcgg gcgtcgggca cgccgaagac gcaacattcg gccaccgcgg gatgctggtg
     2461 gatcgtcgcc tcgacctcgg cgcagtagat gttctcgccg ccgcgcagga ccatgtcctt
     2521 cttgcggtcg accaggaaga tgaagccgtc ct
//
LOCUS       DAORNX010000424         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30693_length_2550_cov_6.347495, whole genome shotgun sequence.
ACCESSION   DAORNX010000424 DAORNX010000000
VERSION     DAORNX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30693_length_2550_cov_6.347495"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLO65_14685"
     CDS             <1..759
                     /locus_tag="PLO65_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRD29543.1"
                     /translation="RHPLARALADAASSGEVATDVVEVAGMGVEGVIDGRRARLGRAA
                     FLGVGSRHADETELWFGFDDNTRIRFTFEDRLRPDAAAAVAELKRLGLTVEVLSGDVA
                     GPVARAALEAGVDHWTAGLTPFDKVAALDRLKQSGRKVLMVGDGLNDAAAIARAHASM
                     APGSAVDASQNAADLVFSGKRLDAVPFSVTVARAARWRALENFAFSALYNVIAAPAAM
                     FGLVNPLVAAVAMSGSSLVVTLNALRMNLTGGRR"
     gene            756..920
                     /gene="ccoS"
                     /locus_tag="PLO65_14690"
     CDS             756..920
                     /gene="ccoS"
                     /locus_tag="PLO65_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRD29544.1"
                     /translation="MTIVLLLAPFSLLLALTGLAAFWWTIRSGQYEDPQGDAARILDD
                     HLADGPDPDS"
     gene            984..2366
                     /gene="hemN"
                     /locus_tag="PLO65_14695"
     CDS             984..2366
                     /gene="hemN"
                     /locus_tag="PLO65_14695"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRD29545.1"
                     /translation="MTSVASAHTFDARRRDLLDRYDGNVPRYTSYPTAVEFSSIVDED
                     VAAAWLGQLPLDERLSVYVHIPFCKRLCWYCGCNTRAVRNRTLISDYIAFLGEELALV
                     EKALPGRPTAGAIHLGGGTPNMLSREDLGALFRGLRHVFRVSPGAEIAAELDPSVLTR
                     EWVVAAAFHGLTRASVGVQDLSPHVQQAVNRIEPFEVVERAVGWLREAGVASVNLDLM
                     YGLPRQRVEDVLATLDKVLTLRPERLALFGYAHVPWVKAHQKLIEASDLPGAAERLDQ
                     SEAAAAKLTDEGYVRIGLDHFALPDDPLALALQAGTLRRNFQGYTADPHMTLIGLGAS
                     SISSLPQGYYQNDATELGWRRRLQEGRLPVVRGVALTDEDRFRGDIIETLMCRRSVDL
                     AQVCARHGRDLETLAEEETALRGMAADGLVALAPGRVEMTRLGQPWLRSVARVFDRRS
                     GAQSRFSRVL"
     gene            complement(2371..>2550)
                     /locus_tag="PLO65_14700"
     CDS             complement(2371..>2550)
                     /locus_tag="PLO65_14700"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRD29546.1"
                     /translation="TFVTVGYGDIVLGPAWRLLSAIEAANGVILVAWSTAFLIAVTAR
                     LRALEHDWLKPRDPD"
BASE COUNT          351 a          915 c          891 g          393 t
ORIGIN      
        1 cgccacccgc tcgcccgcgc gctcgccgac gccgccagca gcggcgaggt cgcgaccgac
       61 gtcgtcgaag tcgccggcat gggcgtggag ggcgtcatcg acggccggcg cgcgcggctc
      121 ggccgggccg ccttccttgg cgtgggctcg cgccacgccg acgaaaccga gctgtggttc
      181 ggcttcgatg acaacacccg gattcggttc accttcgagg accgcctgcg tccggacgcc
      241 gccgccgccg tggcggagct caagcggctg ggcctgacgg tcgaggtcct gtcgggggac
      301 gtcgccggac cggtcgcccg cgcggcgctg gaggccggcg tcgaccactg gacggccggc
      361 ctgacgccgt tcgacaaggt cgccgcgctc gaccggctca agcagtcggg tcgcaaggtg
      421 ctgatggtcg gcgacggcct caacgacgcg gccgccatcg cacgggcgca cgcctcgatg
      481 gccccgggat cggccgtcga cgccagccag aacgcagccg atctggtgtt ctcgggcaaa
      541 cgactcgacg cggtgccctt cagcgtgacg gtggcgcgcg cggcccgctg gcgggcgctg
      601 gagaatttcg ccttctcggc cctctacaac gtgatcgccg cgccggcggc gatgttcggt
      661 ctggtcaatc cgctggtcgc cgcggtagcc atgtccggct cgtcgctggt cgtgacgctg
      721 aacgcgctgc gcatgaacct gaccggaggt cgccggtgac catcgtcctg ctgctggccc
      781 cgttctccct gctgctggcc ctcacgggtc tggccgcctt ctggtggacc attcgctcgg
      841 gccagtacga ggacccgcag ggcgacgccg cgcgaatcct cgacgatcac ctcgccgacg
      901 gtcccgatcc ggactcttga tgcagatcac ggtgactccg cccggaaacg gcaaattgcc
      961 gccattgtcc gggattttcg cttatgacct cagtcgcctc cgcccacacc tttgacgccc
     1021 gccgacggga tctgctggat cgctatgacg gcaacgtgcc gcgctacacc agctatccca
     1081 cggcggtcga attctcgagc atcgtggacg aggacgtggc cgcggcctgg ctgggccagc
     1141 tgccgctcga cgagcgcctg tcggtctacg tccatatccc gttctgcaag cgcctgtgct
     1201 ggtactgcgg ctgcaacacc cgggcggtgc gcaacaggac gctgatcagc gactacatcg
     1261 ccttcctcgg cgaagagctg gcgctggtcg agaaggcgct gcccggccgc ccgacggcgg
     1321 gcgccatcca tctgggcggc ggcacgccca acatgctgtc ccgcgaggac ctcggcgccc
     1381 tgttcagggg cctgcgccat gtcttcaggg tctcgccggg agccgagatc gccgccgagc
     1441 tcgacccctc ggtgctcacc cgcgagtggg ttgtcgccgc cgccttccac ggcctgaccc
     1501 gggccagcgt cggcgtgcag gacctttccc cccacgtcca gcaggcggtc aatcgcatcg
     1561 aacccttcga ggtcgtggaa cgggcggtgg gctggctgcg cgaggcgggc gtggcctcgg
     1621 tcaatctcga cctcatgtac ggcctgccgc gccagcgggt cgaggacgtg ctggcgacgc
     1681 tcgacaaggt gctgaccctg cggcccgaac gtctggccct gttcggctac gcccacgtgc
     1741 cgtgggtcaa ggcgcatcag aagctgatcg aagcctccga cctgcccggg gcggccgaac
     1801 ggctggacca gagcgaagcc gcggcggcga agctgaccga cgaaggctac gtccgcatcg
     1861 gcctggacca tttcgccctg ccggacgatc cgctggcgct cgcgctgcag gccgggacgc
     1921 tgcgacgcaa cttccagggc tatacggccg acccgcacat gaccctgatc ggcctcggcg
     1981 cctcgtcgat cagcagcctg ccgcagggct actaccagaa cgacgccacc gagctcggct
     2041 ggcggcgccg tctgcaggag ggacgtctgc cggtggtgcg cggcgtggcc ctgaccgacg
     2101 aggatcgctt tcgcggcgac atcatcgaga ccctgatgtg ccgtcgctcg gtcgatctgg
     2161 cccaggtctg cgcgcggcac gggcgtgacc tcgagaccct ggccgaggag gagacggcct
     2221 tgcgcgggat ggccgcggac gggctcgtgg cgctcgcgcc gggccgggtg gaaatgacca
     2281 ggctcggcca gccgtggctg cgctcggtgg cgcgcgtgtt cgaccggcgc agcggcgccc
     2341 agtcgcgctt ttcgcgcgtg ctctgacggt tcagtccggg tcgcgcggct tgagccagtc
     2401 gtgctcgagc gcgcgcagtc gcgccgtcac cgcgatcagg aaggccgtcg accaggcgac
     2461 gaggatcacg ccgttggccg cctcgatcgc gctcaacagc cgccaggccg gcccgaggac
     2521 aatgtcgccg tagcccacgg tgacaaaggt
//
LOCUS       DAORNX010000425         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30694_length_2550_cov_3.870140, whole genome shotgun sequence.
ACCESSION   DAORNX010000425 DAORNX010000000
VERSION     DAORNX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30694_length_2550_cov_3.870140"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..>1283
                     /gene="gcvPB"
                     /locus_tag="PLO65_14705"
     CDS             174..>1283
                     /gene="gcvPB"
                     /locus_tag="PLO65_14705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRD29547.1"
                     /translation="MLNTQGRPTSAGEPTATTHPTFTGNRALQQTEPLLFEVGRPETT
                     GVDLPEVPAHTPRLGGLARKGAIGLVGLSEPETMRHYVRLSQRNYGIDTGLFPLGSCT
                     MKHNPRLNEAMARLPGFADVHPLQPQSTVQGAVALMEMLSHWLMTLTGMPAVALSPKA
                     GAHGELCGMMAIKAAIEARGEAATRTVVLVPDSAHGTNPATAALIGFSVRAVPAGPDG
                     TVHVDEVRRRLGPDVAAIMLTNPNTCGLFEPEIVEIAAAMHEAGAYFYCDGANFNAIV
                     GKTRPGDLGIDAMHINLHKTFSTPHGGGGPGAGPVVLSADLAPFAPAPWVVHHPGGFD
                     LVEGPAGEAASAFGRMSAFHGQMGMFVRAYAYMRSH"
     assembly_gap    1284..1489
                     /estimated_length=206
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1490..1738
                     /locus_tag="PLO65_14710"
     CDS             <1490..1738
                     /locus_tag="PLO65_14710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518835.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRD29548.1"
                     /translation="MYFPLVVHGAMLIEPTETESKQELDRFIHALRALAGAATAGEVD
                     RFKGAPFHAPLRRLDETLAARKPRLRWRPETIPPLAAE"
     gene            1893..2270
                     /locus_tag="PLO65_14715"
     CDS             1893..2270
                     /locus_tag="PLO65_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29549.1"
                     /translation="MPRAFTPADDAARDLLARATRWLLLGLGLLIIAVGVLIAPLPGP
                     GGIPVIVVGLMVTLRNSFWAKRQFIRFQRAHPKMVFPIRRLLRREPEVMPVVWQGVLR
                     MERVILPRNRRFVKKVRKHFRRR"
     gene            complement(2372..>2550)
                     /locus_tag="PLO65_14720"
     CDS             complement(2372..>2550)
                     /locus_tag="PLO65_14720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077534.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HRD29550.1"
                     /translation="LAERKIASAEAQAAADVKAVAADLAAQAAEQILAARLAAAGTDA
                     SVDEAIKGMAARLQ"
BASE COUNT          351 a          805 c          787 g          401 t
ORIGIN      
        1 ccggccgcgg tgatcgtcga acagctcgcg gaacagcgaa tcctcggcgg tgttccggtc
       61 tcgagactgc tgccgaatgc cggtttcgag catgacctca tcgtctgttc gacagaagtg
      121 aacacggacg cggatcgcgc ggcctttgtt gccgcactcg gggaggtgct gtgatgctga
      181 acactcaggg acgcccgacg tccgccggtg agccgacggc caccacccac cccaccttca
      241 ccggcaaccg cgcgcttcag cagaccgagc ctttgctgtt cgaggttggt cgtccggaaa
      301 caaccggcgt tgatctgccg gaggttccgg cccatacgcc gcgcctcggc ggcctggccc
      361 gcaaaggcgc gatcggcctt gtcggcctgt cggaacccga gacgatgcga cactatgtcc
      421 gcctcagcca gcgcaactac ggcatcgaca ccgggctgtt tccgctgggc tcctgcacca
      481 tgaagcacaa tccccgactg aacgaagcga tggcgcggct gcccggcttt gccgatgtcc
      541 atccgctcca gccgcagtcg acggtgcagg gggcggtggc cttgatggag atgctcagcc
      601 actggctcat gacgctgacc ggcatgccgg ctgttgcgct gtctcccaag gccggcgcac
      661 atggggaact ctgtggcatg atggcgatca aggctgccat cgaggcccgt ggcgaggcgg
      721 cgacccgcac ggtggttctg gtgccggact cggcgcatgg caccaacccg gccacggcgg
      781 cgctgatcgg cttttcggtt cgcgccgtgc cggccggacc ggatggtacg gtgcatgtcg
      841 atgaggtgcg caggcgtctc ggtccggatg tcgcggcgat catgctgacc aacccgaaca
      901 cctgtggcct gttcgagccg gagatcgtcg agattgccgc tgcgatgcat gaagccggtg
      961 cgtatttcta ctgcgacggc gccaatttca acgcgatcgt cggcaagacc aggcccggcg
     1021 atcttggcat cgatgcgatg cacatcaacc tgcacaagac cttctccacg ccccacggcg
     1081 gcggcgggcc gggcgccggc ccggtggtgc tgagcgcgga cctggcgccg ttcgccccgg
     1141 ccccctgggt ggtccaccac ccgggcggat tcgacctggt cgagggcccg gccggcgagg
     1201 ccgccagcgc ctttggccgg atgagcgcct tccacggcca gatgggcatg ttcgtgcggg
     1261 cctacgccta catgcgcagc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgtatttccc
     1501 gctggtcgtc cacggggcca tgctgatcga gccgaccgag acggagtcga agcaggagct
     1561 tgaccgcttc atccacgccc tgcgcgccct ggccggcgcg gcgacggccg gcgaggtcga
     1621 ccgtttcaag ggggctccgt tccacgcccc gctgcgccgt ctcgacgaga ccctggccgc
     1681 ccgcaagccg cggttgcgct ggcgacctga gacaattccg ccactggcgg ccgaatagcc
     1741 gtgcagggcg cgtgaagagt ttgtcataag gggaacgggc gtctttacgc ctcgttgtcg
     1801 gctgcacata tccaatgatg cagcgacgcg gactcacggg aaagcggcgt gctcagcccg
     1861 cgttcacgtg atccatgtcc ctagtcctga cgatgccccg cgcctttacg cccgccgacg
     1921 acgccgcccg cgacctgctg gcgcgcgcga cccgctggct gctgctgggc ctggggctgc
     1981 tgatcatcgc cgtcggcgtg ctgatcgcgc ccctgcccgg accgggcggc attccggtga
     2041 tcgtcgtcgg ccttatggtc accctgcgca acagcttctg ggccaagcgc cagttcatcc
     2101 gcttccagcg ggctcacccg aagatggtct ttccgatccg ccgcctgctg cgccgcgagc
     2161 cggaagtcat gccggtggtg tggcagggcg tgctgcggat ggaacgggtc atccttccgc
     2221 gcaaccggcg cttcgtgaag aaggtgcgca agcacttccg ccggcgctag gcgaagaaaa
     2281 ggggacatgt accgcaggta cgtgtccccc aatccgcatt ctgatttccg attgtcgggg
     2341 acacggacct tcggaccatg tccccttcga tctactgcag gcgggcggcc atgcccttga
     2401 tggcctcgtc gaccgacgcg tccgtcccgg ccgccgccag gcgagcggcg aggatctgtt
     2461 cggcggcctg ggcggcgaga tcggcggcca ccgccttgac gtcggcggcg gcctgggcct
     2521 cggccgaggc gatcttgcgc tcggccaggg
//
LOCUS       DAORNX010000426         2541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30839_length_2541_cov_7.675784, whole genome shotgun sequence.
ACCESSION   DAORNX010000426 DAORNX010000000
VERSION     DAORNX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30839_length_2541_cov_7.675784"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLO65_14725"
     CDS             complement(<1..212)
                     /locus_tag="PLO65_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD29551.1"
                     /translation="MTIPADPRLILRDEELDSGLELMLLAEASLWAAVDLALEGEPGL
                     GRPHWRAAFLLRRRPGLGVRDLSRLTG"
     gene            293..1180
                     /locus_tag="PLO65_14730"
     CDS             293..1180
                     /locus_tag="PLO65_14730"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRD29552.1"
                     /translation="MSIVPFDDRDGWIWLDGQFVPWREAKVHVLTHAMHYASAVFEGE
                     RMYGGEIFELTAHTERLFNSAEILDFRIPYTVSEIDEACKATCARNGLTDCYVRPIAW
                     RGSEMIGVSAQNTKIHVAIAVWDWPSYFDPEQKKKGIRLTWAKYKRPSPETAPTASKA
                     AGLYMICTISKHAAEKEGYADALMLDWRGYVAEATGANVFFVRDGVIHTPTPDCFLDG
                     ITRRSVIRLARQRGFEVVERHIPREDMATFSECFIVGTAAEVTPVAEVGEFRFTPGNI
                     SLSLMDDYARLVRRELVDA"
     gene            complement(1293..1634)
                     /locus_tag="PLO65_14735"
     CDS             complement(1293..1634)
                     /locus_tag="PLO65_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29553.1"
                     /translation="MGARLLSFVLGLMLGGFGWAIVDPKGLAGARLPALDLGMFEGHR
                     AFIGWGLLALGIASLLSAVLPKGEGKAAPRKGPPVVDFNALPEPDVRQEAEAPAHPVR
                     LSSSPTPSALW"
     gene            complement(1770..2165)
                     /locus_tag="PLO65_14740"
     CDS             complement(1770..2165)
                     /locus_tag="PLO65_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29554.1"
                     /translation="MTDVTDRPHEDRVLPAVVYALYLLSLPSAWVAVLIGLVLAYANR
                     DTAGPRMATHYQFLIQTFWKSIWWAVIGVVLVIVGGIFSVLLIGIPVLIAGGLILAGV
                     HIWFYVRVAVGLIYLAQDQPYPRPDAWLF"
     gene            complement(2230..>2541)
                     /locus_tag="PLO65_14745"
     CDS             complement(2230..>2541)
                     /locus_tag="PLO65_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29555.1"
                     /translation="DRMETFSPLVRAIAATAADRAPRARFATAGLEMGEAFVWTMGLL
                     GIGAAVLLVASISAGAVSLGLDLSARLLFVLILMLAALPWLRRGPATLDPRAIPRALL
                     P"
BASE COUNT          445 a          865 c          875 g          356 t
ORIGIN      
        1 ccggtcaggc gcgacaggtc ccgcacgccc aggcccggcc ggcgccgcag caggaaggcg
       61 gcccgccagt ggggcctgcc gagccccggc tcgccctcca gcgccaggtc gaccgccgcc
      121 cacaggctgg cttcggccag cagcatcagc tccaggccgg agtcgagttc ctcgtcgcga
      181 aggatcagcc gcggatcggc cgggatggtc actgcactgc accattttgt ttgacgccaa
      241 agggcctggg gcgtctaagg tcgcccttcc cttgaaagga gggtttctcc caatgtctat
      301 cgttcccttc gatgatcgcg acggttggat ctggctcgat ggccagttcg tgccctggcg
      361 cgaggcgaag gtgcacgtac tgacacacgc catgcactac gcctcggccg tgttcgaggg
      421 cgagcgcatg tacggcgggg aaatcttcga gctgacggct cataccgagc ggctgttcaa
      481 ttcggcggaa atcctcgact tcaggatccc ctacacggtg tcggagatcg acgaggcctg
      541 caaggcgacc tgcgccagga acggtctcac ggattgttac gttcgtccga tcgcctggcg
      601 cggctcggaa atgatcggcg tctcggccca gaacacgaag atccacgtgg ccatcgcggt
      661 gtgggactgg cccagctatt tcgatcccga gcagaagaag aagggcatcc gtctgacctg
      721 ggcgaagtac aagcgcccgt cgccggagac cgcgccgacc gcttcgaagg ccgccggcct
      781 gtacatgatc tgcaccatct ccaagcatgc cgcggagaag gagggctacg ccgacgccct
      841 gatgctcgac tggcgcggct atgtcgccga agccaccggg gccaatgtgt tcttcgtgcg
      901 cgacggggtg atccacacgc cgacgccgga ctgcttcctc gacggcatca cgcgccgcag
      961 cgtgatccgc ctggcgcggc agcgcggctt cgaggtggtc gaacggcata tcccgcgcga
     1021 ggacatggcg accttcagcg aatgcttcat cgtcggcacc gccgccgagg tgacgccggt
     1081 ggccgaggtg ggcgagttcc gcttcacccc gggcaacatc tcgctgtcgc tgatggacga
     1141 ctatgcccgc ctggtaaggc gcgaactggt cgacgcctga cacgttcgcg gggggacatg
     1201 cacttcagtg cgtgtcccct tcttcagaag aacctgaggc gccggcaggg gacatgcact
     1261 tcagtgcgtg tccccgcatc ccccgcatcc cctcaccaca gcgccgacgg cgtcggtgaa
     1321 ctggacaggc ggacagggtg ggccggcgcc tcggcctcct ggcggacatc cggttccggc
     1381 agggcgttga agtcgacgac gggcggcccc ttgcgcgggg ccgccttgcc ctcgcccttg
     1441 ggcagcacgg ccgacagcag cgaggcgatc cccagcgcca gcagccccca gccgatgaac
     1501 gcccggtgcc cctcgaacat gcccaggtcg agcgccggaa gccgtgcgcc ggccagcccc
     1561 ttcggatcga cgatggccca gccgaagccg cccagcatca ggcccagcac aaagctcagg
     1621 agtcgcgcgc ccatggtctg tccccgttcg gtccccaacg ccaggcgagg gttcggcctg
     1681 gcggggccgc ggtcaaaagg ctgcaaccgt caggggaagg ggcggttgaa agccgttcgt
     1741 tcgttgagca gacccgaggc ggcctgccct taaaacagcc acgcgtcggg ccggggatag
     1801 ggctggtcct gcgccaggta gatcaggccc accgccaccc ggacgtagaa ccagatgtgc
     1861 acgccggcca ggatcaggcc gccggcgatc agcaccggga tgccgatcag caacaccgag
     1921 aagatgccgc cgacgattac cagcaccacg ccgatcaccg cccaccagat cgatttccag
     1981 aaggtctgga tcaggaactg gtagtgggtc gccatgcgcg gcccggccgt gtcgcggttg
     2041 gcgtaggcca gcaccagccc gatcagcacg gccacccagg ccgacggcag gctcagcaga
     2101 taaagggcgt agacgaccgc gggcaggacg cggtcttcat gcgggcggtc ggtgacatcg
     2161 gtcatgaggg gcctccggga cggatgctgg acggtttcgg cgcaactggc aaaccgcgga
     2221 cggccagggt cacgggagca gcgcgcgggg aatggcacgc ggatcgaggg tcgccgggcc
     2281 gcgccgcagc cagggcaggg cggccagcat caggatcagc acgaacagca gccgggccga
     2341 caggtccagt cccagcgaca ccgcgccggc gctgatcgag gcgaccagca gcacggccgc
     2401 cccgatcccc agcaggccca tggtccagac gaaggcctcg cccatctcaa ggccggcggt
     2461 ggcgaagcgg gcgcggggcg cgcggtcggc ggcggtggcg gcgatcgcgc ggaccagggg
     2521 ggaaaacgtc tccatgcggt c
//
LOCUS       DAORNX010000427         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_30938_length_2535_cov_5.134274, whole genome shotgun sequence.
ACCESSION   DAORNX010000427 DAORNX010000000
VERSION     DAORNX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30938_length_2535_cov_5.134274"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1202
                     /locus_tag="PLO65_14750"
     CDS             9..1202
                     /locus_tag="PLO65_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29556.1"
                     /translation="MPALQPTPLDHADPDETFLAEVRAFLAEALTPDLREAGRRTLGV
                     HSDIAACRLWHQRLHRRGWIAPGWPVAHGGAGWTPRQRFLFDRECALNDAPVLFAGGI
                     RSLGPLIIEMGTAMQQARYLPAILSGEDLWAQGFSEPGAGSDLARVATRAVRQGERYV
                     IDGSKIWTTGAHLSNRMFALVRTQTGEKPQHGLTFLLLDMASPGLTVSPIITLDGEHE
                     LNAVFFDQVSVPVANRIGEEGQGWAVAKRLMQLARSNNTPAALVRRVYQRARAAALAA
                     TDLEPGLWARLVAAEIELTAFEQTELALLPGGRLREGDSTSPSVLKLIGSELHQRVAE
                     LAMEIAGALRGPALEPFRETDGLLADSARATAKHFATRAASIYSGSSETQRNVIAQAL
                     SPRDR"
     gene            complement(1199..1900)
                     /locus_tag="PLO65_14755"
     CDS             complement(1199..1900)
                     /locus_tag="PLO65_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD29557.1"
                     /translation="MKTLAIISRKGGTGKTTLAVHLAVAAHASGYATLLADTDRQHSA
                     VEWRRQRQDCQPAVEPVKPGALFARQQEAARAGVGLLVIDTGPSIEEEVEQAVRCADL
                     CLVVARPNFFDIRAVAESAALAARFHRPVAFVLNQAPHRQQSIEAPVLMEAVSALRGL
                     GFPVAPIGLRTRVAYQRSVAAGSTALALEPSSSAAGEIAALWTWVEGRLQAERPAVRM
                     PTFIAATDRTIPLPL"
     gene            complement(1954..>2535)
                     /locus_tag="PLO65_14760"
     CDS             complement(1954..>2535)
                     /locus_tag="PLO65_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD29558.1"
                     /translation="QAGGLLSFRLHDDPDTTRAFIDALELCTVAVSLGEPLTLIWPYR
                     DGLIRLAVGDLQPTLGAVRRGGRSVLLDQGTAVLGDDETILQAFRRLNPAASVNDAQA
                     ALARFLFRNTAAHQSVGSLSGGERLRAALACVLSAPEPPQLLILDEPTNHLDLASIEA
                     VEAALAGYDGALLVVSHDRDFLEAIDVSREIVL"
BASE COUNT          392 a          892 c          912 g          339 t
ORIGIN      
        1 tggcgataat gccggccctt cagcccacgc ccctggacca cgccgacccc gacgagacct
       61 ttctcgccga ggtgcgcgcc tttctggccg aagccttgac gcccgacctg cgcgaggccg
      121 gtcggcggac gctgggagtc cattccgaca tcgccgcctg ccggctgtgg caccagcggc
      181 tgcaccggcg gggctggatc gcgccgggct ggcccgtggc ccatggcggc gccggctgga
      241 cgccccgcca gcgtttcctg ttcgaccgcg agtgcgcgct gaacgacgct cccgtgctgt
      301 ttgccggcgg catccgcagc ctggggccgc tgatcatcga gatgggcacg gcgatgcagc
      361 aggcccgata tctgccggcc atcctgtcgg gcgaggacct ctgggcccag ggattctccg
      421 aaccgggcgc gggatctgac ctggcgcggg tcgccacccg ggccgttcga cagggcgaac
      481 gctatgtgat cgacggatcg aagatctgga ccacaggcgc ccatctgtcg aaccgcatgt
      541 tcgccctggt gcgcacccag accggcgaga agccgcagca cggcctgacc ttcctgctgc
      601 tcgacatggc ttcgcccggg ctgaccgtct ccccgatcat caccctggac ggcgagcacg
      661 agctcaacgc cgtgttcttc gaccaggtca gcgtgcccgt ggccaaccgg atcggcgagg
      721 agggccaggg ctgggccgtg gccaagcggc tgatgcagct ggcccgctcg aacaacacgc
      781 cggcggccct ggtgcggcgg gtctatcagc gcgcgagggc cgcggccctc gcggcgacgg
      841 atctcgagcc gggcctgtgg gcgcgcctcg tggccgccga gatcgagctg acggccttcg
      901 aacagaccga actggccctg ctgccgggcg gccggctgcg cgagggcgac agcaccagcc
      961 cgtcggtgct caagttgatc ggcagcgaac tccaccagcg ggtggccgag ctggcgatgg
     1021 agatcgccgg cgccctgcgc ggaccggcgc tggagccgtt ccgcgagacc gatggcctcc
     1081 tcgccgacag cgcgcgcgcg acggccaagc atttcgccac ccgggcggcg agcatctatt
     1141 ctggatcgag cgaaacccag cgcaacgtta tcgcccaggc cctgtcgccg cgcgatcgct
     1201 agaggggcag cggaatggtc cggtcagtgg cggcgatgaa ggtcggcatg cgcacggcgg
     1261 ggcgttcggc ctgcagccgt ccctcgaccc aggtccacag ggcggcgatc tcgccggccg
     1321 cggagctgga aggctcaaga gccagagccg tcgagccggc cgcgacactg cgctggtagg
     1381 cgacccgcgt gcgcaggccg atcggcgcga ccggaaaccc cagaccccgc agggcggaca
     1441 ccgcctccat cagcacgggc gcctcgatcg actgctgccg atgcggcgcc tggttgagga
     1501 cgaaggccac cggcctgtgg aagcgggcgg ccagcgccgc cgattccgcc accgcgcgga
     1561 tgtcgaagaa gttgggccgc gcgaccacca ggcagaggtc ggcgcagcga accgcctgct
     1621 cgacctcctc ctcgatcgac gggccggtat cgatgaccag caggccgacc ccggcgcggg
     1681 ccgcttcctg ctgccgggcg aacagggcgc ccggcttgac cggctcgacg gcaggctgac
     1741 agtcctggcg ctggcggcgc cactcgacgg ccgaatgctg acgatcggta tcggccagca
     1801 gcgtggcgta gcccgacgca tgggccgcga cggccagatg cacggccagg gttgtcttcc
     1861 cggtcccgcc tttgcgggaa atgatggcga gtgtcttcaa cgcctgtccc cgaacgaaac
     1921 gctcgggccc ccgcgcagca ggaaccgtgc ccgctacagg acgatctccc gggagacgtc
     1981 gatggcttcg aggaagtccc ggtcgtggga gaccaccagc agcgcgccgt cgtagccggc
     2041 cagcgccgcc tcgaccgcct cgatcgaggc gagatccaga tggttggtcg gctcgtcgag
     2101 gatcagcagc tgcggcggtt ccggcgccga caggacgcaa gccagggcgg cccgcaggcg
     2161 ctcgccgccg ctcagcgagc ctacggactg atgcgcggcg gtgttgcgga acaggaaccg
     2221 ggccagggcc gcctgggcgt cgttgacgct ggcggcggga ttcagccggc ggaaggcctg
     2281 cagaatggtc tcgtcatcac cgagcacggc cgtcccctgg tcgagcagca ccgatcggcc
     2341 gccgcgccgg acggcgccga gtgtcggctg aaggtcgccg accgccagcc ggatcagccc
     2401 gtcgcgatag ggccagatca aggtcagcgg ctcaccgagg ctgacagcga cggtgcagag
     2461 ttcgagggcg tcgatgaagg cacgggtggt atcgggatcg tcgtgcaatc ggaacgagag
     2521 cagtccacca gcctg
//
LOCUS       DAORNX010000428         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31000_length_2531_cov_7.547658, whole genome shotgun sequence.
ACCESSION   DAORNX010000428 DAORNX010000000
VERSION     DAORNX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31000_length_2531_cov_7.547658"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLO65_14765"
     CDS             complement(<1..190)
                     /locus_tag="PLO65_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HRD29559.1"
                     /translation="MARQILLIGALGAALMTSACGEEHPADPVVATAPAAGRLTVAAT
                     TIADLKPVPATLTTRDMGE"
     gene            complement(225..587)
                     /locus_tag="PLO65_14770"
     CDS             complement(225..587)
                     /locus_tag="PLO65_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD29560.1"
                     /translation="MFGSPKTRDLTPHQVKTALDGRQILLIDVREPAEFAAERIHGAL
                     NFPLSTFDPAALPDAGDKLVVFQCGSGKRSATAVDHCRKAGHAIDTHLAGGIMAWKAA
                     GLPTVTLDSETGALRDRR"
     gene            complement(599..922)
                     /locus_tag="PLO65_14775"
     CDS             complement(599..922)
                     /locus_tag="PLO65_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD29561.1"
                     /translation="MNFEAHSLKASADQACALLKALANPHRLMIVCSLLEREQSVGAL
                     AQLLGVRETLASQHLGLLRRDGVVSARRDGQTIYYALQSGQARALVETLSNLFCAVDG
                     PPARS"
     gene            1084..2364
                     /locus_tag="PLO65_14780"
     CDS             1084..2364
                     /locus_tag="PLO65_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD29562.1"
                     /translation="MARPRIVILGAGLGGAIAAFEIKDAVGDRADVSVVSQGDTFHFV
                     PSNPWVAVHWRKREAIEVKLPPVFSKKGVAFTGVGARRVNPAANQIELGDGSTLDYDY
                     LVIATGPELAFDEIKGLGPHGGFTQSICHVDHAESAAAAFDRFVENPGPIVVGAVQGA
                     SCFGPAYEFAMILDTELKRRKIRDRVPMTFVTSEPYVGHLGLDGVGDTKGLLESEMRE
                     RHIKWITNARVASVDDGMMHVEEIADDGAVKATHDVPFAYSMMLPAFRGVEAVRGIEG
                     LTNPRGFIVVDKHQRNPTFPNVFALGVCVAIAPTGKTPVPVGVPKTGFMIESMVTAIA
                     HNLGDLVAGRPAAREATWNAICLADFGDGGVAFVAQPQIPPRNVNWSASGKWVHLAKV
                     GFEKYFLAKVRRGESEPFYEKLALDVMGIRKIKP"
BASE COUNT          404 a          865 c          872 g          390 t
ORIGIN      
        1 cctcgcccat gtcgcgggtg gtcagggtcg cgggaaccgg cttgaggtcg gcgatggtcg
       61 tggccgcgac cgtcagccgg ccggccgcgg gcgcggtcgc caccaccgga tcggccggat
      121 gctcctcgcc gcaggctgac gtcatcaggg ccgcgccgag ggcgccgatc agcaaaatct
      181 gtcttgccat gggaatccgg tcatccgtgc tggagcggcg ggagctagcg gcggtcgcgc
      241 agggcgccgg tttcgctgtc gagggtcacg gtcggcagcc ccgcggcctt ccaggccatg
      301 atgccgccgg ccaggtgggt gtcgatcgcg tgaccggcct tgcggcagtg gtcgaccgcg
      361 gtggccgagc gcttgcccga gccgcactgg aacaccacca gcttgtcgcc ggcgtcgggc
      421 agggcggccg gatcgaaggt cgacagcggg aagttcaggg cgccgtggat acgctcggcg
      481 gcgaactccg ccggctcgcg gacgtcgatc agcaggatct gccggccgtc gagggcggtc
      541 ttcacctggt gcggggtcag gtcccgggtc ttgggagagc cgaacatcgg agtttccttc
      601 agctgcgggc gggcggtcca tcgaccgcgc aaaacaggtt ggacagggtt tcgaccagcg
      661 cgcgggcctg gccgctctgc agggcgtagt agatggtctg gccgtcgcgc ctggccgaga
      721 cgacgccgtc gcggcgaagc agtcccaggt gctgggaggc cagggtttcg cgcacgccca
      781 gcagctgggc gagcgcgccg accgactgtt cccgctcgag cagcgaacag acgatcatca
      841 gccgatgcgg attggccagc gccttgagca gggcgcaggc ctggtccgcc gaggccttca
      901 gactgtgggc ttcaaaattc atagttgcgt atatgcgtct attcacatat tcagtcaagc
      961 cagtcacgcg atcaccctag gttcgatcca cgggccccga tgtgatccgt atcaaagcca
     1021 actgcccgga agtggcgaac ccttgcgcca gccaaccggg agcttcgtcg gaggagacag
     1081 accatggcca ggccacgaat cgtcattctg ggcgccggac tgggcggcgc gatcgccgcg
     1141 ttcgagatca aggacgccgt gggcgatcgg gccgacgtct cggtcgtgtc ccagggcgac
     1201 accttccact tcgtaccgtc caatccctgg gtcgccgtcc actggcgcaa gcgcgaggcc
     1261 atcgaggtca agctgccgcc ggtgttcagc aagaagggcg tggccttcac cggcgtcggc
     1321 gcccggcgcg tgaacccggc ggccaaccag atcgaactcg gcgacggctc gaccctcgac
     1381 tacgactacc tggtcatcgc caccggcccc gaactggcct tcgacgagat caagggcctg
     1441 gggccgcatg gcggcttcac tcagtcgatc tgccatgttg accatgccga gtcggcggcc
     1501 gccgccttcg accgcttcgt cgagaaccct ggccccatcg tggtcggggc ggtccagggc
     1561 gcctcctgct tcggcccggc ctacgaattc gcgatgatcc tcgacaccga gctcaagcgc
     1621 cgcaagatcc gcgacagggt gccgatgacc ttcgtcacct ccgagcccta cgtcggccat
     1681 ctgggcctcg acggcgtcgg cgacaccaag ggcctgctcg agagcgagat gcgcgagcgg
     1741 catatcaagt ggatcaccaa cgccagggtc gcctcggtcg acgacggcat gatgcacgtc
     1801 gaggagatcg ccgacgacgg cgcggtcaag gccacccacg acgtcccgtt cgcctattca
     1861 atgatgctgc cggccttccg cggcgtggag gcggtgcgcg gcatcgaggg cctgaccaat
     1921 ccgcgcggct tcatcgtggt cgacaagcac cagcgcaacc cgaccttccc caatgtcttc
     1981 gccctcggcg tctgcgtcgc catcgccccg accggcaaga cgccggtgcc ggtcggcgtg
     2041 cccaagaccg gcttcatgat cgagagcatg gtcaccgcca tcgcccataa cctgggcgac
     2101 ctggtcgccg ggcgtccggc cgcgcgcgaa gcgacctgga acgcgatctg cctggccgac
     2161 tttggcgacg gcggggtggc cttcgtcgcc cagccgcaga ttccgccgcg caacgtcaac
     2221 tggtcggcca gcggcaagtg ggtccacctg gccaaggtcg gcttcgaaaa atacttcctc
     2281 gccaaggtgc gccgcggcga gagtgagccg ttctacgaga agctcgccct cgacgtgatg
     2341 ggcatccgca agatcaagcc ctgagccgga ggatcgtgat gacgacccgc cagttcgtct
     2401 gcccggcctg cgcggccgcc aaccgcgcgc cggagggcaa ggatgcgcgc aaggccaagt
     2461 gcggccgctg cggcgcggcg ctgttcaccg gccacgcggt ggaggtcggc ggccaggcct
     2521 tcagggccca c
//
LOCUS       DAORNX010000429         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31437_length_2507_cov_6.596656, whole genome shotgun sequence.
ACCESSION   DAORNX010000429 DAORNX010000000
VERSION     DAORNX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31437_length_2507_cov_6.596656"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLO65_14785"
     CDS             <1..214
                     /locus_tag="PLO65_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29563.1"
                     /translation="SDNRAAASLAKAKASELAHLVSNEMVQMHGGIGMTDVHDAGLYL
                     KRARVAEQLFGGAGYHRDRYARVLGF"
     gene            216..1052
                     /locus_tag="PLO65_14790"
     CDS             216..1052
                     /locus_tag="PLO65_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29564.1"
                     /translation="MKVGGTVAVITGGASGIGFGIAQALARRGAKVVLADIEAERALA
                     AAETLRDQGFEATSCYLDVIDQASWDATADVAFGHWDQAPQLLFNNAGVGGGTTVHET
                     PERVWDWVFSVNIRGVYLGVRTFAPRMLASGLPGRIVNTASEHALGLPESKRGGIVAP
                     YTAAKHAVMGYSLCMRRDFEGTSLSAGVICPGVVQSDIWNAFRNRHPDFGGPRSLERP
                     GPHPMAHGLAAATAGERIADQVETDDFFIFTNGPHEAEVLGDYVGEATAAMAAFRQRY
                     GV"
     gene            1113..1616
                     /locus_tag="PLO65_14795"
     CDS             1113..1616
                     /locus_tag="PLO65_14795"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD29565.1"
                     /translation="MSTDINDRAEVENRLWHEIGKHGHTVMLGVHTHPMQHFQPMTAL
                     VEKERGQIWFFTRTDTELARSIQEGKSAMMVVQNGQTFQACVAGELTLQHDPGRIETY
                     WNPVVAAWYPQGKDDPKLTLLCLDVSDAQVWISQGGPIRFAFEIAGANATGEKPDVGG
                     SRRLDLN"
     gene            complement(1629..>2507)
                     /locus_tag="PLO65_14800"
     CDS             complement(1629..>2507)
                     /locus_tag="PLO65_14800"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29566.1"
                     /translation="RPAVADGAPIPLWPEGTDLAKPDTGDGPEVTGKGSSPVGGRDWH
                     WVGDVSQPTMTLYHPRGRNTGTTMLVLPGGGFSIVAMDLEGSEICDWAVRQGMTCVVL
                     KYRAPQKWPWDERRKHGRRPEVLLALQDAQRAMGLLRERAGIYGINPDRIGVIGFSAG
                     AYLAADVSNIEGRTYPPVDAADRRPSRPAFAIMAYTGRLWAGGSSSDLGLAPWVKISP
                     RAPPTLLIHAMDDPVDDIRHPMAYALALHEAGAPVDVRFYAKGGHAYGMRPTADPVTT
                     QWPGQAEQWLRDLGLL"
BASE COUNT          390 a          882 c          870 g          365 t
ORIGIN      
        1 gagcgacaac cgcgcggccg cctccctggc caaggccaag gccagcgagc tggcgcatct
       61 ggtcagcaac gagatggtcc agatgcacgg cggcatcggc atgaccgacg tccacgacgc
      121 cggcctctac ctcaagcgcg cgcgggtggc cgagcagctg ttcggcgggg ccggttacca
      181 ccgcgaccgc tacgcgcgcg tgctgggctt ctgaggtgaa ggtcggcggg acggtcgcag
      241 tcatcacggg cggagcgtcg ggcatcggct tcggcatcgc ccaggccctg gcccggcgcg
      301 gcgcgaaggt ggtgctggcg gacatcgagg ccgaacgggc gctggccgcc gccgagaccc
      361 tgcgcgacca ggggtttgaa gccaccagct gctatctcga cgtgatcgac caggccagct
      421 gggacgccac ggccgacgtc gccttcggcc actgggacca ggccccgcag ctgctgttca
      481 acaacgccgg cgtcggcggc gggaccacgg tgcatgagac gcccgagcgg gtctgggact
      541 gggtgttctc ggtcaacatc cgcggcgtct atctgggggt caggaccttc gcgccgcgca
      601 tgctggccag cggcctgccc ggcaggatcg tcaacaccgc ctccgaacac gccctgggcc
      661 tgccggagtc gaagcggggc gggatcgtcg cgccctatac ggccgcaaag cacgcggtca
      721 tgggctattc cctgtgcatg cgccgcgact tcgagggcac cagcctgtcg gccggcgtga
      781 tctgtccggg cgtggtgcag agcgacatct ggaacgcctt ccgcaaccgc caccccgact
      841 tcggcggccc gcgctcgctg gagcggcccg gtccccaccc gatggcccac ggcctggccg
      901 ccgccacggc cggcgaacgc atcgccgacc aggtcgagac cgacgacttc ttcatcttca
      961 ccaacggccc gcacgaggcc gaggtgctcg gcgactatgt cggcgaggcg accgccgcga
     1021 tggcggcgtt ccggcagagg tacggggtct agccggaacc gccctccccg cccggggttc
     1081 tcctagcctt agtgcagtac gggagaccca ccatgagcac cgacatcaac gaccgcgccg
     1141 aggtcgagaa ccgcctgtgg cacgagatcg ggaagcacgg ccacacggtg atgctcggcg
     1201 tccataccca tccgatgcag cacttccagc cgatgacggc gctggtcgag aaggagcggg
     1261 gccagatctg gttcttcacc cgcaccgaca ccgagctcgc caggtccatc caggagggca
     1321 agtcggccat gatggtcgtg cagaacggcc agacgttcca ggcctgcgtc gccggcgagt
     1381 tgaccctgca gcacgacccc ggccggatcg agacgtactg gaacccggtg gtcgccgcct
     1441 ggtatccaca gggcaaggac gacccgaagc tgaccctgct gtgcctggac gtcagcgacg
     1501 cccaggtctg gatcagccag ggcggcccga tcaggttcgc gttcgagatc gccggggcca
     1561 acgccaccgg cgagaagccc gatgtcggcg gcagccggcg gctggacctg aactagggtc
     1621 cgggtccctt aaaggaggcc gaggtcgcgc agccactgtt cggcctggcc gggccactgc
     1681 gtggtgacgg gatcagccgt cggccgcatg ccataggcgt ggccgccctt cgcgtagaag
     1741 cgcacgtcga ccggcgcccc tgcttcatgc aacgcgagcg cgtatgccat cggatggcgg
     1801 atatcgtcga ccgggtcgtc catggcgtgg atcagcagcg tcggcggggc cctggggctg
     1861 atcttcaccc agggggccag ccccaggtcg ctgctggatc cgccggccca aaggcgcccc
     1921 gtataggcca tgatcgcgaa ggccggccgc gagggtcgcc ggtcggcggc gtcgacgggc
     1981 gggtaggttc gcccttcgat attgctcacg tcggccgcca gataggcgcc ggcggaaaac
     2041 ccgataacgc cgatcctgtc ggggttgatt ccgtagatcc cggcccgttc ccgcagcagt
     2101 cccatggcgc gctgggcgtc ctgcagggcc agcaacacct cgggccgccg gccgtgcttc
     2161 cggcgttcgt cccacggcca tttctgcggc gcgcggtact tgaggacgac gcaggtcatg
     2221 ccctggcgca cggcccagtc gcagatttcg gagccctcga ggtccatcgc cacgatcgag
     2281 aacccgccgc ccggcaggac cagcatcgtc gtaccggtat ttcgtcccct gggatgatag
     2341 agggtcatgg ttggctggct gacgtcgccc acccaatgcc agtctctccc gcccactgga
     2401 ctggagccct tgccggtcac ttcgggacca tccccggtgt cgggcttcgc cagatcggtc
     2461 ccctccggcc agagcgggat gggggcgccg tcggcgacgg ccggccg
//
LOCUS       DAORNX010000430         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31565_length_2499_cov_7.844517, whole genome shotgun sequence.
ACCESSION   DAORNX010000430 DAORNX010000000
VERSION     DAORNX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31565_length_2499_cov_7.844517"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLO65_14805"
     CDS             complement(<1..982)
                     /locus_tag="PLO65_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29567.1"
                     /translation="MTDDAPYRLHGLKLSYFTGKLEAYLRAKGVPFRFIEMDTADFRA
                     CARATGIAQMPQLEGPDGRWMTDTTAIIARFEATGPGPAFRPTDPAAAFLCLFLEDAF
                     DEWLWRPALYYRWAFAEDARLMGGQIARTMLRDLPGPLWLRSLVIRRRQQRVYLRQDG
                     VTSATAPVIEDLYRQVLARLEPIFASRPFLCGARPCIADFGLFGAMFRHFASDPTPAA
                     IMRETAPNVLAWTARLWALTPEQAAAAAPVETVPADLDPLLRLAADDHLTELAVNAEA
                     VAAGRARVEFNHRGVRWRLSPSPYRARCLADLRTAFAALSPSDRSAVAARL"
     gene            complement(979..>2499)
                     /locus_tag="PLO65_14810"
     CDS             complement(979..>2499)
                     /locus_tag="PLO65_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD29568.1"
                     /translation="MLLTGVDNHRAGVATIEEILPPSQKGKPGYGLQFAPGVWTIARR
                     LKADGYRTLMVGKWHLGHENGALPNFHGFDRSLALDASGADNWAAKPYMPYYDSAPWF
                     EDGRPAKMPKAYYSSDMMVDRLQAYIDEQPAGAQPFFAYLAFQAVHIPVQAPKEYVDH
                     YRGRFDGGWAAMRRARAERARALGLLPADATIRGLPDDARDWDALTPKERAIYARSME
                     VYAGMIEAMDANIGRLIAHLEARGQLANTLFVVTSDNGPEPSDPVHAKGMNLWMATHG
                     YRWKLDGLGGPGSLNFIGREWASAASSPGPRYKFYASEGGIRVPLILSGPGVKPGTRV
                     DSPAFAIDIAPTLVDYAAAGARPEAVSMDGRTLRPVLNGTATVTHPEDSPVGIEVSGN
                     AALFRGSHKIVRDMPPVGDGRWRLYDLSKDPGETTDLATAQPALFRQMLDDYAAYEVR
                     VGVQPLPAGYTTDRQLLSNAMGKQRATMIIMGLGLVGLLVLLAGGVWLGLRRLRRR"
BASE COUNT          367 a          850 c          919 g          363 t
ORIGIN      
        1 ccaggcgggc ggccacggcg ctccggtccg agggggacag ggccgcgaag gcggttctca
       61 ggtccgccag gcagcgcgcg cgatagggtg acggtgacag gcgccagcgc acgccgcgat
      121 gattgaactc gacccgcgcc cggcccgccg cgaccgcctc ggcgttgacc gccagttcgg
      181 tcaggtgatc gtcggccgcc agcctcagca gaggatcgag gtctgccgga acggtctcca
      241 ccggcgccgc cgcggcggcc tgctccggcg taagagccca gagccgcgcc gtccaggcca
      301 gcacgttcgg cgccgtctcc cgcatgatcg ccgcgggcgt ggggtcagag gcgaagtggc
      361 ggaacatggc gccgaacagg ccgaaatccg cgatgcaggg gcgcgcgccg cagagaaagg
      421 gccgcgaggc gaagatcggc tccagtcgcg cgaggacctg cctgtagagg tcttcgatta
      481 ccggcgcggt ggccgaggtg acgccgtcct gccgcaggta gacccgctgc tggcggcgcc
      541 ggatgaccag ggaccgcagc cacagggggc cgggcaggtc cctcagcatc gtccgggcga
      601 tctggccgcc catcagtcgc gcatcctcgg cgaaggccca gcggtagtag agcgccggcc
      661 gccacagcca ttcgtcgaag gcgtcctcga ggaacaggca gaggaaggcg gcggcgggat
      721 cggtcggccg gaaggcgggg ccgggcccgg tcgcctcgaa ccgggcgatg atcgccgtgg
      781 tgtcggtcat ccagcgcccg tcgggaccct ccagctgcgg catctgggcg atgccggtcg
      841 cccgggcgca ggcgcgaaaa tcggccgtgt ccatctcgat gaaccggaac ggcacgccct
      901 tggcgcgcag ataggcttca agcttgccgg tgaagtagga cagcttgagt ccgtgcaggc
      961 ggtagggcgc gtcgtcggtc atcgacggcg gagtcgccgc aggcccagcc agacgccgcc
     1021 cgccagcagg accagcaacc cgaccaggcc gaggcccatg atgatcatcg tcgcccgttg
     1081 cttgcccatc gcgttgctca gcagctggcg gtcggtcgtg tagcccgcgg gaagcggctg
     1141 gacgcccacg cgaacctcgt aggcggcgta gtcgtcgagc atctggcgga acagggcggg
     1201 ctgagccgtg gcgaggtcgg tggtttcacc cgggtccttc gacaggtcat agagtcgcca
     1261 ccggccgtcg ccgaccggcg gcatgtcccg gacgatcttg tggctgccgc ggaacagggc
     1321 ggcgttgccg gacacctcga tgccgacggg gctgtcctcc gggtgggtga cggtcgccgt
     1381 tccgttcagc acgggccgca gcgttcgccc gtccatgctg accgcctccg gcctggcccc
     1441 cgcggcggcg tagtcgacca gggtcggggc gatgtcgatg gcgaaggccg gtgaatccac
     1501 gcgggttccc ggcttcaccc cgggtccgga caggatcagc ggcacgcgga tgccgccctc
     1561 cgaggcatag aacttgtagc gtggccccgg cgaagaggcc gccgaggccc actcgcggcc
     1621 aatgaagttc aggctgcccg gtcccccgag accgtcgagc ttccagcggt agccatgggt
     1681 ggccatccac aggttcatgc ccttggcgtg caccggatcg ctcggttccg gtccgttgtc
     1741 cgaggtgacc acgaacaggg tgttggcaag ctggccccgc gcctcgaggt gcgcgatgag
     1801 ccggccgatg ttggcgtcca tcgcctcgat catcccggcg tagacctcca tcgagcgggc
     1861 gtagatggcc cgctccttcg gggtcagcgc gtcccagtcg cgggcgtcgt ccggaagtcc
     1921 gcggatcgtg gcgtcggcgg ggagaaggcc caacgcccgg gcccgctcgg ctcgcgccct
     1981 gcgcatcgcg gcccagccgc cgtcgaagcg gcccctgtag tggtcgacat attccttggg
     2041 ggcctggacc ggaatgtgca ccgcctggaa ggcgagatag gcgaagaacg gctgggcgcc
     2101 cgcgggctgt tcgtcgatgt aggcctgcag ccggtcgacc atcatgtccg aggagtagta
     2161 ggccttcggc atcttcgccg gccggccgtc ctcgaaccac ggcgcgctgt cgtaataggg
     2221 catgtagggc ttggcggccc agttgtcggc ccccgaggcg tcgagcgcca gcgaacggtc
     2281 gaagccgtgg aagttgggca aggcgccgtt ctcgtgcccc aggtgccact tgccgaccat
     2341 cagggtccgg tagccgtccg ccttcagccg cctggcgatg gtccagacgc ccggggcgaa
     2401 ctgcaggccg tagcccggtt tgcccttctg cgacggcggc aggatctcct cgatcgtcgc
     2461 cacgccggcc cggtggttgt cgacgccggt cagcagcat
//
LOCUS       DAORNX010000431         2498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31581_length_2498_cov_7.718788, whole genome shotgun sequence.
ACCESSION   DAORNX010000431 DAORNX010000000
VERSION     DAORNX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31581_length_2498_cov_7.718788"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLO65_14815"
     CDS             <1..890
                     /locus_tag="PLO65_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD29569.1"
                     /translation="PGAGILKDALGPILLFLAAFAIAAAALARRVLTALRTLAANDAK
                     LEAALQDLTLARDRAEAANVAKSQFLANISHEIRTPMNGVLGMAQIMDRGDLAPTQRQ
                     HLAVIRESGATLLTLLNDVLDLAKIEAGKLEIEREEADIGAAVTAVCGTFAGLAGEKG
                     LAFDLAIDPEADGVWRIDAMRIKQVLGNLIANAVKFTSEGRVSVQVRATADGLAFAVS
                     DTGAGIAPERVADLFAKFNQLDASTTRQFGGTGLGLAICRELVELMGGEMAVESEPGR
                     GSCFSFRLPAEPVRRPVTA"
     gene            983..>2498
                     /gene="cobT"
                     /locus_tag="PLO65_14820"
     CDS             983..>2498
                     /gene="cobT"
                     /locus_tag="PLO65_14820"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HRD29570.1"
                     /translation="MANKPESPTEPFKRALAHAARSLAETPDLEVVFSGDGPSLSGNR
                     AVLPHPPRDLTSKEASRIRGMADQMALRLAHHDPAAHAKGRPASPQGQAIFEAVEQSR
                     IESIGANALGGVRANLTAVLEQSVERKGLARMSDRANAPMADVLALMVRERLTGDAPP
                     DGARAMVDLFREEVEAKAGADLDRLAGSIHDQAAFGRVTRAILRDLDLGDADIDQTET
                     GDEDEGDEDQSQSSESDDGDGDGQTPDGASMEETDGSDSDSQDGEEQMVESEDEATEM
                     SDEAPEMGDGAKPARPDIKDSGQPEPPYKVFTSAHDEVVGAEELCDAEELTRLRAYLD
                     QQLASLSSVVSRLANKLQRRLMAQQNRSWSFDLEEGMLDVARLPRIITDPTAPLSFKQ
                     EEDTEFRDTVVTLLIDNSGSMRGRPIMVAAVCADILARTLERCAVKTEILGFTTRAWK
                     GGVSRDDWIKAGKPAAPGRLNDLRHIVYKGADAPWRRARRNLGLMMREGLLKENI"
BASE COUNT          415 a          901 c          872 g          310 t
ORIGIN      
        1 agcccggcgc cggcatcctc aaggacgccc tgggaccgat cctcctgttc ctcgccgcct
       61 tcgccatcgc cgcggcggcg ctggcgcgcc gcgtgctcac cgccctgcgg accctggccg
      121 cgaacgacgc caagctggaa gcggccctgc aggacctgac gctcgcccgc gaccgggcgg
      181 aggcggcgaa cgtcgccaag agccagttcc tcgccaacat cagccacgag atccgcacgc
      241 cgatgaacgg cgtgctcggc atggcccaga tcatggaccg gggcgacctg gcgccaaccc
      301 agcggcagca cctggccgtc atccgcgagt ccggcgccac cctgctgacc ctgctcaacg
      361 acgtgctcga cctggccaag atcgaggccg gcaagctcga gatcgagcgc gaggaagccg
      421 acatcggcgc cgccgtcacc gccgtctgcg ggaccttcgc cggcttggcg ggcgaaaagg
      481 gcctggcgtt cgacctggcc atcgatccgg aggccgacgg cgtctggcgg atcgacgcga
      541 tgcggatcaa gcaggtgctc ggcaacctga tcgccaacgc ggtcaagttc accagcgagg
      601 gccgggtgtc ggtgcaggtc cgggccaccg ccgacggcct ggcgttcgcg gtcagcgaca
      661 ccggcgccgg gatcgcgccc gagcgggtcg ccgacctgtt cgccaagttc aaccagctcg
      721 acgcctcgac cacgcgacag ttcggcggca cgggtctggg cctggccatc tgtcgcgagc
      781 tggtcgagct gatgggcgga gagatggcgg tcgagagcga gccagggcgt ggatcctgct
      841 tcagtttccg cctgccggcc gaaccggtgc gccgccccgt caccgcctga gggccgggcg
      901 cgcggcgacc gccagacggg acccttccgg cccgaccgcg ccgcccttcc attccgttgc
      961 gtcgtcggag taagacggtt gcatggccaa caagcccgaa agcccgaccg aacccttcaa
     1021 gcgcgccctt gcgcacgccg cccgctcgct cgccgagacg ccagaccttg aagtggtgtt
     1081 ttccggcgac ggcccgtcgc tgtcgggcaa ccgcgccgtg ctgccccatc ccccacgcga
     1141 cctgacctcg aaggaggcgt cgcgcatccg cggcatggcc gaccagatgg ccctgcggct
     1201 ggcgcaccat gaccccgccg cccatgcgaa gggtcggccc gcctcgcccc agggccaggc
     1261 gatcttcgag gccgtcgaac agtcccgcat cgagtcgatc ggcgccaacg ccctgggcgg
     1321 ggtgcgggcc aacctgaccg cggtgctcga acagagcgtc gaacgcaagg ggctggcgcg
     1381 gatgtcggac cgggccaacg cgccgatggc cgatgtgctg gccctgatgg tgcgtgaacg
     1441 gctgaccggc gacgccccgc ccgacggggc cagggcgatg gtcgacctgt tccgcgagga
     1501 ggtcgaggcc aaggctggcg ccgatctcga ccggttggcc ggcagcatcc atgaccaggc
     1561 cgccttcggg agggtcaccc gcgccatcct gcgcgatctc gacctgggcg acgccgacat
     1621 cgaccagacg gagaccggcg acgaggacga gggcgacgag gaccagtccc agagcagcga
     1681 gagcgacgac ggcgacggtg acggccagac tcccgacggc gcctctatgg aggagaccga
     1741 cggctcggac agcgactcgc aggacggcga ggagcagatg gtcgagtccg aggacgaggc
     1801 caccgagatg agcgacgagg cgcccgagat gggcgacggc gccaagccgg cccggccgga
     1861 catcaaggac agcggacagc ccgagccgcc ctacaaggtc ttcaccagcg cccatgacga
     1921 agtggtcggg gcggaggagc tgtgcgacgc cgaggagctg acccggctgc gggcctatct
     1981 cgaccagcag ctggccagcc tgtcgagcgt tgtgtcgcgc ctggcgaaca agctgcagcg
     2041 gcggctgatg gcgcaacaga accgctcctg gagcttcgac ctcgaagagg gcatgctcga
     2101 cgtcgcccgc ctgccgcgga tcatcaccga cccgaccgcg ccgctgtcgt tcaagcagga
     2161 agaggacacc gagttccgcg acacggtcgt taccctgctg atcgacaatt ccggctcgat
     2221 gcgcggccgg ccgatcatgg tggcggccgt ctgcgccgac atcctggccc ggaccctgga
     2281 gcgctgcgcg gtcaagaccg agatcctcgg attcaccacc cgcgcctgga agggcggcgt
     2341 cagccgcgac gactggatca aggccggcaa gcccgccgcc cccggccgac tgaatgacct
     2401 gcgccacatc gtctacaagg gcgccgacgc cccctggcgg cgcgcgcggc gcaatctcgg
     2461 cctgatgatg cgcgaggggc tgctgaagga gaacatcg
//
LOCUS       DAORNX010000432         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31689_length_2491_cov_9.693350, whole genome shotgun sequence.
ACCESSION   DAORNX010000432 DAORNX010000000
VERSION     DAORNX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31689_length_2491_cov_9.693350"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="hutH"
                     /locus_tag="PLO65_14825"
     CDS             complement(<1..1178)
                     /gene="hutH"
                     /locus_tag="PLO65_14825"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRD29571.1"
                     /translation="MAGPVSTALSDGARQAIARSAAAIEAILADGRTVYGVNTGFGLL
                     AKKTIAPDHLTELQRNLILSHCCGVGAPLPDDVVRLILLLKIAGLSRGFSGVRPELIA
                     MLQRLLERDVLPVIPEKGSVGASGDLAPLAHLASTLIGVGELKVAGKVVPATDGLRAA
                     GLEPIVLRAKEGLALINGTQVSTALALKGLVLARRIFEAALVAGALSVDALAGSDVPF
                     DPRIHAARGQPGQIEVARCLRTLLDGSRIRASHADCDRVQDPYSLRCQPQVMGAALDL
                     LTNAERTLLIEAGAVTDNPLVFADTGEALSGGNFHAEPVAFAADALALALAEIGSLSE
                     RRTALLVDPAMSGLPAFLVAGSGVNSGFMIAQVTAAALVSENKGLSHPSSTDSLPTSA
                     "
     gene            complement(1235..>2491)
                     /locus_tag="PLO65_14830"
     CDS             complement(1235..>2491)
                     /locus_tag="PLO65_14830"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRD29572.1"
                     /translation="SQGIVQGTYETFVEVGRQHFGGDLTGKWILTGGLGGMGGAQPLA
                     ATMAGASMLAVECQRRCIERRLETRYLDEATEDLDDALARIDRATKEGRALSVGLLGN
                     AAEVFPELVRRGVRPDVVTDQTSAHDTINGYLPKGWTVERWEQARLSDPASVDRAARA
                     SIMPHVQAMLDFHGMGIPVLDYGNNIRQVAKDEGLEHAFDFPGFVPAYIRPLFCRGVG
                     PFRWAALSGDPEDIYRTDAAMKRLFPDNVHLHRWLDMARDRIAFQGLPARICWIGLGD
                     RHRAGLMFNEMVASGELKAPIVIGRDHLDSGSVASPNRETEGMIDGSDAVSDWPLLNA
                     LLNTAGGATWVSLHHGGGVGMGYSQHAGVVIVADGTPEAARRLERVLWNDPGTGVMRH
                     ADAGYDIAVDCAREQGLDLPSLEAGR"
BASE COUNT          360 a          892 c          882 g          357 t
ORIGIN      
        1 ttggcgctgg tcggcaggct gtcggtgctg gacgggtggc tgaggccctt gttctcgctg
       61 accagggcgg cggcggtgac ctgggcgatc atgaagccgg aattgacccc gctcccggcc
      121 accaggaagg ccggcagccc gctcatcgcc gggtcgacca ggagggccgt cctccgctcg
      181 ctgagcgagc cgatctcggc cagggccaag gccagggcgt cggccgcgaa ggccaccggc
      241 tcggcgtgga agttgccgcc ggacagggcc tcgcctgtgt cggcgaacac cagcgggttg
      301 tcggtcaccg cgccggcctc aatcagcagg gttcgctcgg cgttggtgag caggtcgagc
      361 gccgcaccca tcacctgcgg ctggcagcga aggctgtagg ggtcctgcac ccggtcgcag
      421 tcggcgtggc tggcgcggat ccggcttccg tccaggagcg tgcgcagaca gcgcgcgacc
      481 tcgatctggc cgggctggcc ccgggcggcg tggatgcgcg gatcgaacgg aacgtcgctg
      541 ccggccaggg cgtcgacgct cagggcgccg gcgacgaggg cggcttcgaa aatccgacgg
      601 gccagcacca gccccttcag cgccagcgcg gtggacacct gggtgccgtt gatcagcgcc
      661 agaccctcct tggcgcgcag cacgatcggc tccagtccgg cggccctcag cccgtccgtc
      721 gccggaacga ccttgcccgc cactttcagc tcgccgacgc cgatcagggt ggaggccaga
      781 tgcgccagcg gcgccaggtc gcccgaggcc ccgaccgaac ccttttccgg gatcaccggc
      841 aggacgtcgc gctccagaag ccgctgcagc atggcgatca gttcgggccg cacgcctgaa
      901 aagccgcgcg acaggccggc gatcttcagc agcaggatca gccgcaccac gtcgtccggc
      961 agcggcgcgc cgacgccgca gcagtgcgac aggatcagat tgcgctgcag ctcggtcaga
     1021 tggtccgggg cgatggtctt cttcgccagc aggccgaagc cggtgttgac gccatagacc
     1081 gtgcggccgt cggccaggat cgcctcgatc gcggcggcgg agcgggcgat cgcctgccgg
     1141 gcgccgtcgc tcagggccgt cgacaccggc cccgccatcg cccggcggac ggtctccaga
     1201 tcgatgccgg cgtcgccaag gacaatcgcc gggatcatcg cccggcctcc aggctcggca
     1261 ggtcgaggcc ctgctcgcgg gcgcagtcga cggcgatgtc gtagcccgcg tcggcatggc
     1321 gcatgacgcc cgtgcccgga tcattccaca gcacccgctc cagccgccgg gcggcttccg
     1381 gcgtaccgtc ggcgacaatg accaccccgg catgttgact gtagcccatg ccgaccccgc
     1441 cgccgtggtg cagcgacacc caggtcgcgc cgcccgccgt gttgagcagg gcgttgagca
     1501 gcggccagtc gctgaccgcg tcggagccgt cgatcatgcc ttcggtctcg cggttggggc
     1561 tggccaccga gccgctgtcc agatggtcgc ggccgatgac gatcggggcc ttcagctcgc
     1621 cgctggcgac catctcgttg aacatcagcc cggcccggtg gcggtcgccc aggccgatcc
     1681 agcagatccg cgcgggcagg ccctggaacg cgatccggtc ccgggccatg tcgagccagc
     1741 ggtgcagatg cacattgtcc ggaaacagac gcttcatcgc cgcgtcggtc ctgtagatgt
     1801 cctccggatc gcccgacagg gccgcccagc ggaacgggcc gacaccgcgg cagaacagcg
     1861 ggcggatgta ggccggcacg aagcccggga agtcgaaggc gtgctcgagc ccctcgtcct
     1921 tcgccacctg gcggatgttg ttgccgtagt cgagcaccgg gattcccatg ccgtggaaat
     1981 ccagcatggc ctggacgtgg ggcatgatcg acgcgcgcgc ggcccggtcg accgacgccg
     2041 gatcgctgag ccgcgcctgc tcccagcgct cgacggtcca gcccttcggc agataaccgt
     2101 tgatcgtgtc atgggcgctg gtctggtcgg tgacgacgtc cggccgaaca ccgcgccgca
     2161 ccagttcggg gaacacctcg gccgcgttgc ccagcaggcc gacggacagg gcccgacctt
     2221 ccttcgtcgc cctgtcgatg cgcgcgaggg cgtcgtcgag atcctcggtg gcctcgtcga
     2281 gatagcgggt ctccagccgc cgctcgatgc agcggcgctg gcattcgacg gccagcatcg
     2341 aggcgccggc catggtcgcg gccagcggct gggccccgcc catgccgccg aggcccccgg
     2401 tgaggatcca tttgccggtc aggtcgccgc cgaaatgctg gcggcccacc tcgacgaagg
     2461 tctcgtaggt gccctgaacg atgccctggc t
//
LOCUS       DAORNX010000433         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31798_length_2484_cov_10.711404, whole genome shotgun
            sequence.
ACCESSION   DAORNX010000433 DAORNX010000000
VERSION     DAORNX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31798_length_2484_cov_10.711404"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PLO65_14835"
     CDS             complement(<1..1264)
                     /locus_tag="PLO65_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD29573.1"
                     /translation="MTDLSQIQSVADVARVQASLRPEAEAIWFEGRVTTFGDLDRESN
                     RCAQALLAQGLQPGDRVGVLAKNIDDFFVLWMGCLKARVTLAPVNWRLAPPEIAFILK
                     DAAAKLLVVGADFAGVVDMIVGDLPDIRGLVQFEPGHPRWPAFRDWIGGHPAEDPRLP
                     ADPDDDVIQLYTSGTTGLPKGVQLTQDNFLTLFALALEAGWAKYETGKTNLVVMPLFH
                     VAGTNCGLLALLQGVRNILTREVSAAEIIERLQDKQVAYAFLAPTIINMLLQTPGVEH
                     ADFSRLERIFYGASPISEAVLREAQERFACDFTQLYGLTETIGGATYLPPEDHTPERD
                     KLRSCGKPWPGFEVRVITPTGEVGGPSEVGEIQIRARGVMKGYWNRPEATAEAVCPQG
                     WFSSGDAGYFDDEGYLYIYDRVKDMIVSG"
     gene            complement(1477..>2484)
                     /locus_tag="PLO65_14840"
     CDS             complement(1477..>2484)
                     /locus_tag="PLO65_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD29574.1"
                     /translation="PWNWPVNQIACKVAPALAVGCTMVLKPSEIAPFSAYIWTEILHA
                     AGVPAGVFNLVNGDGPTVGAAISSHPEIDMVSFTGSTRAGIEVARNAAPTVKRVAQEL
                     GGKSPNIILDDADFQAAVTGGVRSVMMNSGQSCNAPTRMLVPGKRMDEVIAIAKAAAE
                     STTVGDPKGNAQMGPVVSEVQFNKIQGLIQKGIDEGATLVTGGVGRPEGLDKGYFVKP
                     TIFANVTNDMTIAKEEIFGPVVSIIGYNSVDEAVKVGNDTEYGLAAYVSGTDVEQLHK
                     VAGKLRAGQVSINGGGGDLMAPFGGYKMSGNGREWGDYGFHEFLETKAMLGYNPAQAA
                     E"
BASE COUNT          356 a          837 c          871 g          420 t
ORIGIN      
        1 cgccgctgac gatcatgtcc ttcacccggt cgtagatgta gaggtagccc tcgtcatcga
       61 aatagccggc gtcgccgctg gagaaccagc cctgcgggca gaccgcctcg gccgtggcct
      121 ccggccggtt ccagtagccc ttcatcacgc cccgggcgcg gatctggatc tcgccgacct
      181 cgctcgggcc gccgacctcg ccggtcgggg tgatcacccg cacctcgaaa cccggccagg
      241 gcttgccgca ggagcgcagc ttgtcgcgct cgggcgtatg gtcctcgggc ggcaggtagg
      301 tggccccgcc gatcgtctcg gtcaggccgt agagctgggt gaagtcgcag gcgaaccgct
      361 cctgggcctc gcgcagcacg gcctcggaga tcggcgaggc gccgtagaag atgcgctcga
      421 ggcggctgaa gtcggcgtgt tccacccccg gggtctgcag cagcatgttg atgatcgtcg
      481 gggcgaggaa ggcgtaggcg acctgcttgt cctgcagccg ttcgatgatc tcggcggcgc
      541 tgacctcgcg ggtcaggatg ttgcgcacgc cctgcagcag ggccagcagg ccgcagttgg
      601 tcccggccac gtggaacagc ggcatcacca cgaggttggt cttgcccgtc tcgtatttgg
      661 cccagccggc ttcgagcgcc agggcgaaca gggtgaggaa gttgtcctgg gtcagctgca
      721 cgcccttggg caggccggtc gtgccgctgg tgtagagctg gatcacgtcg tcgtccgggt
      781 cagccggcag gcgcgggtcc tcggccgggt ggccgccgat ccagtcgcgg aaggccggcc
      841 agcgcgggtg gcccggctcg aactgcacca ggccgcggat gtcgggcagg tcgccgacga
      901 tcatgtcgac caccccggcg aagtccgccc cgaccaccag gagcttcgcg gccgcgtcct
      961 tgaggatgaa ggcgatctcc ggcggggcca gccgccagtt gaccggcgcc agcgtgaccc
     1021 gcgccttcag gcagcccatc cacagcacga agaagtcgtc gatgttcttg gccagcaccc
     1081 cgacccggtc gccgggctgg aggccctggg ccagaagcgc ctgggcgcac cggttcgact
     1141 cacggtccag gtctccgaag gtggtcaccc ttccctcgaa ccagatggcc tcggcctccg
     1201 gccgcaggct ggcctggaca cgcgccacgt cggcgacgga ttggatctgg ctgaggtcgg
     1261 tcatcggttt cctgtcctgc cgcgcctctg ccggacgctg gcggtcctag gatgcacaag
     1321 gcggccgcaa agaaaaaggg ccgccggagc gatccggcga ccctttgcag tctcagcgcg
     1381 tcctttgaca cggccgcggc gcggcctgct caggatgacg tagctctgtg tacgtcatgg
     1441 tgagcagcgc gaagcgcgtg tcgaaccacg caatccctac tccgcagcct gggccgggtt
     1501 gtagccgagc atggccttgg tctcgaggaa ctcgtggaag ccgtagtcgc cccactcgcg
     1561 gccgttgccg ctcatcttgt agccgccgaa gggcgccatc aggtccccgc cgccgccgtt
     1621 gatgctgacc tggccggcgc gcagcttgcc ggccaccttg tgcaactgct cgacgtccgt
     1681 gcccgagacg taggccgcca ggccgtactc ggtgtcgttg ccgaccttca ccgcttcatc
     1741 gacgctgttg tagccgatga tcgagacgac cgggccgaag atctcttcct tggcgatggt
     1801 catgtcgttg gtcacgttgg cgaagatcgt cggcttgacg aagtagccct tgtcgaggcc
     1861 ttccggccgg ccgacgccgc cggtgaccag ggtcgcgccc tcgtcgatgc ccttctggat
     1921 caggccctgg atcttgttga actgcacctc ggagaccacc ggacccatct gggcgttgcc
     1981 cttggggtcg ccgacggtgg tcgactcggc ggccgccttg gcgatggcga tcacctcgtc
     2041 catgcgcttg ccggggacca gcatgcgggt cggggcgttg caggactggc ccgagttcat
     2101 catcaccgag cgcacgccgc ccgtgacggc cgcctggaag tcggcgtcat cgaggatgat
     2161 gttggggctc ttgccgccga gttcctgggc cacgcgcttg acggtcgggg cggcgttgcg
     2221 ggccacctcg atgccggcgc gggtcgagcc ggtgaacgag accatgtcga tctcgggatg
     2281 gctggagatg gccgcgccga cggtcgggcc gtcgccgttg accaggttga acaccccggc
     2341 cggcacgccg gcggcatgca ggatctcggt ccagatgtag gccgagaacg gcgcgatctc
     2401 ggacggcttg agcaccatgg tgcagccgac ggccagcgcc ggggcgacct tgcaggcgat
     2461 ctggttcacc ggccagttcc aggg
//
LOCUS       DAORNX010000434         2483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31813_length_2483_cov_7.747117, whole genome shotgun sequence.
ACCESSION   DAORNX010000434 DAORNX010000000
VERSION     DAORNX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31813_length_2483_cov_7.747117"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLO65_14845"
     CDS             <1..274
                     /locus_tag="PLO65_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29575.1"
                     /translation="GAVIAGSAAELGEAMAMLDRPIDAAVLDANLNGESVGPLAAALH
                     ARGTPFVFATGYGERGAPEGFDAPVVRKPYNVHQIVHALAEATGRA"
     gene            311..2119
                     /locus_tag="PLO65_14850"
     CDS             311..2119
                     /locus_tag="PLO65_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRD29576.1"
                     /translation="MTDRRHDGSPRPAPDAAETRLRLEGIVASAMDAIITVDDRQRIV
                     LFNPAAEHMFGVTSEEAMGEPIARFIPERHRPAHGDHIRRFAATGVTSRQMGSLGAIS
                     GLRGNGQEFPIEASISQVEVGGERLSTVILRDITERKRSDDALQESRRRMEGIVNSAM
                     DALITIDGQQRIVLFNPAAERMFEVDAAEAIGRPISQFIPERFRDGHDAHIRRFRETG
                     VTSRRMGALGAISGVRASGEEFPVEASISQTEVGGEQLATVILRDITERRANEEARLL
                     LAREVDHRAKNALAVVQALVSLTRAPTKEAFVTAVRGRVAALARAHSLLSRNRWQGAD
                     LTQVVTDETAAYQRPGQVRLDGPAISLAPDAVQPISLLIHELATNAVKYGGLSTEAGQ
                     VRVDWRVLPDGQLQLTWTESGGPPVAEPGARGFGSTLVSEVTTRQLGGQVSIDWRATG
                     LCFTVVLPGRNFRAAPASRSAPRGGKGPSSGEPVTQGLILVVEDEVLVAMELCNGLAA
                     VGWDVLGPATTTDEALDLLAGERQPDAAILDINLAGKLVFPLADLLRARGIPFVFCTG
                     YEETGGGDRYRDAPVVRKPVNLETLGQELNRVLTAA"
     gene            complement(2300..>2483)
                     /locus_tag="PLO65_14855"
     CDS             complement(2300..>2483)
                     /locus_tag="PLO65_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD29577.1"
                     /translation="VPHPVFGEVSMQNAFPRLTDTPGAVRWPGPALGEHTDAVLAARA
                     GLSPDELKDLRARGVI"
BASE COUNT          366 a          901 c          872 g          344 t
ORIGIN      
        1 gggggcggtc atcgccggca gcgccgcgga actgggcgag gcgatggcca tgctcgaccg
       61 gccgatcgac gcggcggtgc tggacgccaa tctcaacggc gagtcggtcg ggcccctggc
      121 cgccgccctg cacgcgcgcg gcaccccgtt cgtcttcgcc accggctacg gcgagcgcgg
      181 cgcgccggag ggcttcgacg ccccggtggt ccgcaagccc tacaacgtcc accagatcgt
      241 tcatgcgctg gccgaagcga ccggtcgcgc atgatgacgc accccatgtt cagtacggga
      301 gtcaagcggg atgacagatc gcaggcatga cggatcgccc cgtccggcgc cggacgccgc
      361 cgaaaccagg ctccggctgg agggtatcgt cgcctcggcc atggacgcga tcatcacggt
      421 cgacgaccgg cagcggatcg tcctgttcaa cccggccgcc gagcacatgt tcggcgtcac
      481 cagcgaagag gccatgggag agccgatcgc ccggttcatc cccgagcgcc accgtccggc
      541 ccacggcgac cacatccggc ggttcgccgc caccggcgtc accagccgtc agatgggctc
      601 gctgggcgcc atcagcggcc tgcgcggcaa tggccaggaa ttccccatcg aggcctcgat
      661 ctcgcaggtc gaggtcgggg gcgaacggct gtcgacggtc atcctgcgcg acatcaccga
      721 acgcaagcgg tcggacgacg cgctgcagga aagccggcgc cgcatggagg gcatcgtcaa
      781 ctcggcgatg gacgccctga tcaccatcga cggccagcaa cggattgtcc tgttcaaccc
      841 cgcggccgag cgcatgttcg aggtcgacgc ggcggaggcg atcggccggc cgatttccca
      901 gttcatcccc gagcgcttcc gcgacggcca cgacgcccat atccgccggt tccgggagac
      961 gggggtcacc agccgccgca tgggcgccct gggcgcgatc agtggcgtgc gcgcgtccgg
     1021 cgaggaattt ccggtcgagg cctcgatctc ccagaccgaa gtcggcggcg agcagctggc
     1081 gaccgtgatc ctgcgcgaca tcaccgagcg cagggccaac gaagaggccc gcctgctcct
     1141 cgcccgcgaa gtcgatcacc gggccaagaa cgccctggcc gtggtccagg cgctggtctc
     1201 cctgacccgc gccccgacca aggaggcctt cgtcaccgcc gttcgcgggc gggtcgcggc
     1261 cctggcccgg gcccattcgc tgctctcgcg caatcgctgg cagggcgccg acctgacgca
     1321 ggtggtgacc gacgagaccg ccgcctacca gcgaccgggc caggtccggc tcgacgggcc
     1381 ggcgatctcc ctggcccccg acgccgtcca gccgatcagc ctgctgatcc acgagctggc
     1441 caccaatgcg gtcaagtacg gcggcctgtc gaccgaggcg gggcaggtca gggtcgactg
     1501 gcgcgtcctg cccgacggcc agctgcagct gacctggact gaatccggcg gcccgccggt
     1561 ggccgagccc ggcgcccggg ggttcggctc gaccctggtg tccgaggtca ccacgcgaca
     1621 actgggcgga caggtgtcga tcgactggcg tgcgacgggc ctgtgcttca cggtggtgct
     1681 gccgggccgg aatttccgcg ccgctcccgc ttcaaggagc gcgccccgcg gcggcaaggg
     1741 cccctcttcg ggcgagccgg tcacccaggg cctgatcctg gtcgtcgagg acgaggttct
     1801 ggtggcgatg gagttgtgca acgggctggc ggcggtcggc tgggacgtgc tgggaccggc
     1861 caccaccacc gacgaggccc tcgacctgct ggccggcgaa cgccagccgg acgcggccat
     1921 cctcgacatc aacctggccg gaaagctggt cttcccgctg gccgatctgc tgcgggcgcg
     1981 cggcattccg ttcgtcttct gcaccggcta cgaggagacc ggcggcggtg atcgctaccg
     2041 ggacgcgcct gtggtgcgaa agccggtcaa tctcgagacc ctcggccagg agctgaaccg
     2101 ggtgctgacc gcggcctagg cgaatcgacg ctcgcgatcc ggggtcgttt tctcaccgat
     2161 tgtccccgcg aaggcgggga cccaagccct ggtggccggc gaagccgcgc gtccagacct
     2221 gtagcaccgc ctgggttccc gccttcgcgg gaacattcgg agcccttggc ggctcacgcg
     2281 cgctgaacgc cgattggacc tagatcacgc cccgcgcgcg caggtccttc agctcgtccg
     2341 gcgacagccc ggcgcgcgcg gccagcaccg cgtcggtgtg ttcgcccagg gcggggcccg
     2401 gccagcgcac ggcgcccggc gtgtcggtca gcctgggaaa ggcgttctgc atgctgacct
     2461 cgccgaacac cgggtgcggc acc
//
LOCUS       DAORNX010000435         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31927_length_2476_cov_7.030153, whole genome shotgun sequence.
ACCESSION   DAORNX010000435 DAORNX010000000
VERSION     DAORNX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31927_length_2476_cov_7.030153"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1702
                     /locus_tag="PLO65_14860"
     CDS             92..1702
                     /locus_tag="PLO65_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRD29578.1"
                     /translation="MSNATALEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGA
                     VRARGENRRTRSDWMKIERERGISVSASVMTFDHAGLMFNLLDTPGHEDFSEDTYRTL
                     TAADAAVMVLDAAKGIEPQTLKLFEVCRLRDIPIVTFINKMDREAQDPFALLDEIAGK
                     LALDPAPLYWPAGSGGRFKGMMDLRHQTLIPFGKNREDGHPAPVPFRSNAIDALLSPD
                     ERDELEEGAMLVQEASRPFDPQAFLEGHMTPVIFGSALRHFGVDELLATLAAYAPPPR
                     PRNAQRAGADTHVAPGDPEVTGFVFKVQANMDPNHRDRIAFFKLTSGRFVRGMKLKTF
                     STGVRGEAPGKQMSINAPIMFFASDRELAEEAYAGDVIGIPNHGVLRVGESLSESGTV
                     RFAGLPNFAPEILQRVRVKDPLKAKHLKKALEGLAEEGVTQLFRPILGSDFIVGAVGQ
                     LQFEVMADRLSNEYQLDVVFEPAPYAEARWLSGSAASIETFASTHRTAMAEDIDNSPV
                     FLGKSAWEINYVAERYPDLQFLRTRERG"
     gene            1771..2115
                     /locus_tag="PLO65_14865"
     CDS             1771..2115
                     /locus_tag="PLO65_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29579.1"
                     /translation="MLSPVMVLTITVLFAAAPAAPPPWPESLDCAALTQAASELHKDP
                     VTGIPAEPEFDHAMFWSFAAMDAARLAGLSSAQAEADQKTERARIKPLLAAGDPGAKA
                     RLAACVAQVPPI"
     gene            2254..>2476
                     /locus_tag="PLO65_14870"
     CDS             2254..>2476
                     /locus_tag="PLO65_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29580.1"
                     /translation="MFEFGRELRRFLGGEPVAGVWRDGLTGGDQTLLELLDLRLLINE
                     ARGADVAAGRIGVKDRGARLLEAAIAWREV"
BASE COUNT          377 a          894 c          841 g          364 t
ORIGIN      
        1 ggggccagtt ggccacgccc gcccccgacg ccggggatga ggcgtcgccc gacgcgtgac
       61 aaacgccggc cggaccgcta cccccgccgc catgtccaac gccaccgccc tcgaagccgc
      121 gcgccgacgc accttcgcca tcatcagcca tcctgacgcc ggcaagacga ccctgaccga
      181 gaacctgctg ctggccggcg gggccatccg ggcggccggg gccgtgcgcg cccgcgggga
      241 gaaccgccgc acccggtcgg actggatgaa gatcgagcgc gagcgcggca tctcggtcag
      301 cgccagcgtc atgacgttcg accacgccgg cctgatgttc aacctgctcg acacgccggg
      361 ccacgaggac ttctcggaag acacctaccg caccctgacc gccgccgacg ccgccgtcat
      421 ggtgctggac gcggccaagg gcatcgagcc ccagaccctg aagctgttcg aggtctgccg
      481 cctgcgcgac atcccgatcg tcaccttcat caacaagatg gaccgcgagg cccaggaccc
      541 gttcgccctg ctggacgaga tcgccggcaa gctggcgctg gaccccgcgc cgctctactg
      601 gccggccggg tcgggcgggc ggttcaaggg catgatggat ctgcgccacc agacgctgat
      661 ccccttcggc aagaaccgcg aggacggcca ccccgcgccc gtgccattca ggtcgaacgc
      721 catcgacgcc ctgctgtcgc ccgacgaacg cgacgagctg gaggagggcg ccatgctggt
      781 ccaggaggcg tccaggccgt tcgacccgca ggccttcctc gaggggcaca tgacgccggt
      841 gatcttcggc tcggccctgc gccacttcgg cgtcgacgag ctgctcgcga ccctggccgc
      901 ctatgctccg ccgccccggc cgcgcaacgc ccagcgcgcc ggcgccgaca cccatgtcgc
      961 gcccggcgat cccgaggtca ccggcttcgt gttcaaggtc caggcaaaca tggaccccaa
     1021 ccaccgcgac cggatcgcct tcttcaagct gaccagcggc aggttcgttc gcggcatgaa
     1081 gctgaagact ttctcgaccg gcgtgcgcgg cgaggcgccg ggcaagcaga tgagcatcaa
     1141 cgcgccgatc atgttcttcg cctcggaccg cgagctggcg gaggaagcct acgccggcga
     1201 cgtcatcggc attcccaacc acggcgtgct gcgggtcggc gagagcctgt cggagagcgg
     1261 cacggtgcgc ttcgccggcc tgcccaactt cgcgccggaa atcctccagc gcgtgcgggt
     1321 caaggatccg ctcaaggcca agcatctgaa gaaggcgctg gaaggcctgg cggaggaggg
     1381 ggtgacccag ctgttccgcc cgattcttgg ctcggacttc atcgtcggcg ctgtcggcca
     1441 gctgcagttc gaggtgatgg ccgaccggct gtcgaacgaa taccagctgg acgtggtgtt
     1501 cgagccggcc ccctatgccg aagcccgctg gctgtcggga tccgccgcca gcatcgagac
     1561 cttcgccagc acccaccgca cggccatggc cgaggacatc gacaattccc cggtgtttct
     1621 cggcaagtcg gcctgggaga tcaactacgt ggccgaacgc taccccgacc tgcagttcct
     1681 gcggacccgg gagcgaggct agcccccggt ttggggcatg cgcgcgccgg ccggcgatgg
     1741 tctgcatgac gggtgcaatg gaggcccgcc gtgctctctc ccgtcatggt gctgacgatc
     1801 accgtcctgt tcgccgccgc cccggccgcc ccgccgccct ggcccgagag cctggactgc
     1861 gccgccctga cccaggcggc gtcggagctg cacaaggacc ccgtcaccgg gatcccggcc
     1921 gagcctgagt tcgaccacgc catgttctgg tcgttcgcgg cgatggacgc ggcgcggctg
     1981 gcgggcctgt cctcggccca ggccgaggcc gaccagaaga ccgaacgggc gaggatcaag
     2041 cccctgctgg ccgccggtga ccccggcgcg aaggcccggc tggccgcctg tgtggcgcag
     2101 gtcccgccga tctgacgttt aaggcgtcgc gttaaccata ccggcaaagt taatggcttg
     2161 ttaacggctt gggaccgggc tttgctcctc tcctgaagac cgcccgccgg gtgttccggg
     2221 tcgggacgga attcgccggg gagcaggcgc cgcatgttcg agttcggtcg tgagttgcgt
     2281 cgttttctcg ggggcgagcc tgtcgccggc gtctggcggg atggcctgac cggtggcgac
     2341 cagacgctgc tggagctgct cgatctgcgg ctgctgatca acgaggctcg cggcgccgac
     2401 gtcgccgccg ggcggatcgg cgtcaaggac cgcggcgcgc ggctgctgga agcggccatc
     2461 gcctggcgcg aggtcg
//
LOCUS       DAORNX010000436         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_31966_length_2474_cov_4.485738, whole genome shotgun sequence.
ACCESSION   DAORNX010000436 DAORNX010000000
VERSION     DAORNX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31966_length_2474_cov_4.485738"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1182)
                     /locus_tag="PLO65_14875"
     CDS             complement(178..1182)
                     /locus_tag="PLO65_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRD29581.1"
                     /translation="MDSQFQVGRRRKARATVAVAVAAGGLAMAAPALASEYGLSPYAL
                     GSQTSMAGFTPPPGIFVTDTVISYQGDADDTLQLPLGARLAAGVDEEVLVNALTIAVF
                     PDVRLGSGQIGFAATLPYGRVGVDAAATFVGPLGLPGGAASDEETAIGDLQLAALIGW
                     KDGVHNWNLSGALVLPTGEYDSARLANVGLNRTAFDAKGAYTYMNPGTGREFSAGAGF
                     TFNGENKDTDYQSGTDFHLEGSFTQHLPSGWALGVGGYFYQQITGDSGSGARLGDFKG
                     RVGALGPMVSYTFMIGPAPVQLSGRWYREFGAENRAEGDTVMFGVSMPIAAFGAPPAN
                     "
     gene            1730..2302
                     /locus_tag="PLO65_14880"
     CDS             1730..2302
                     /locus_tag="PLO65_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HRD29582.1"
                     /translation="MTASNPDSRSLTRRQLASGLLVAGAACLPATANAGPALDGEADA
                     ALARLYAANAKARELGQRARAVLIFPSIAKAGLLVGGQTGKGVLQTVGEPARYYRITA
                     ASYGLQAGAQTYSYALFFMTRSSLDYLHVSDGWSIGTGPSVVFIDEGFARTLNTTTLT
                     QDVYAIAFGQRGLMAGTGLEGAKISPISAP"
BASE COUNT          399 a          822 c          847 g          406 t
ORIGIN      
        1 cggagcgccc gaggcggcga cgatcgaggc ggcggccacg ccggcgggga tcatcgacag
       61 gagtccccgg cgcgaaggcc cgtccgactt ggaattgttg ttgttttgat tggtcatggt
      121 gttctcccgg ccgggttcgc cgggggcttc cccggctgtc cgcctcagtg ggttcgatca
      181 gttcgcgggg ggcgcgccaa aggcggcgat gggcatggaa acgccgaaca tcacggtgtc
      241 gccctcggcc cggttctcgg cgccgaattc ccggtaccag cggccgctga gctgcaccgg
      301 cgcggggccg atcatgaagg tgtagctgac catcggtccc aaggcgccca ctcgcccctt
      361 gaagtcgcca agcctggcgc ccgaccccga gtcaccggtg atctgctggt agaaatagcc
      421 accgaccccc aacgcccagc cagacggaag atgctgggtg aaggaccctt ccaggtggaa
      481 atccgtcccg ctctggtagt cggtgtcctt gttctcgccg ttgaaggtga agccggcgcc
      541 ggccgagaat tcgcgtccgg tgcccgggtt catgtaggtg taggcgccct tggcgtcgaa
      601 ggcggtccgg ttcaggccga cgttggccag ccgggccgag tcgtactcac cggtcggcag
      661 caccagggcg ccgctcaggt tccagttgtg gactccgtcc ttccagccga tcagggcggc
      721 cagctgcaga tcgccgatgg cggtttcctc gtccgaggcg gcgccgcccg gcaggccgag
      781 cgggccgacg aaggtcgcgg ccgcatcgac cccgacccgg ccatagggca gggtcgccgc
      841 gaagccgatc tggccactgc cgagccggac gtccggaaac acggcgatcg tcagggcgtt
      901 gaccagcact tcctcgtcga cgccggcggc caggcgggcg ccgagcggaa gctgcagcgt
      961 atcgtccgcg tcgccctggt agctgatgac cgtgtcggtc acgaagattc cgggcggcgg
     1021 cgtgaagccg gccatcgagg tctgggagcc cagcgcatag ggcgagaggc cgtactcgct
     1081 cgcgagagcg ggcgccgcca tcgcgaggcc gccggccgcc acggcgaccg cgacggtggc
     1141 gcgcgccttg cggcggcgcc cgacctgaaa ctgactatcc atctcgacct cctgcgggac
     1201 cgaacggcgc aatgggttcg gcgcgaatgc tggcgtgcgg ggtcgtggag tcggcctcgc
     1261 cacgcgacct caccgaagtt actggtgtct caggtcttta atatcaggta ttcaccccat
     1321 tcccgcgaac tacatcttga acaactgtaa aagaagtctc cgggacatcg atcagcaggg
     1381 gaatcccacc agaattttct gggcaactgt tcattaggcg gttccgggcg tgacccattg
     1441 tgggcgacca ggcgacggaa tcggcggcgc agaagggcgt tggcgcccct tacccggaac
     1501 atcaggcatg attctgcccg ttgccatggc tttggggagt ttcgtcctga cggcgggttt
     1561 caaggtcttt ccaggcaaag aaccggtccg gagctacctg aaatggtaat ggggtaatga
     1621 cctaatagtc ttcgaggatt tggcggatac gctctgccga cgaattggga cgatgctccg
     1681 tccaggggcg gcccaacctg tcgaccatct cgtcaagggg actccaaccg tgaccgcatc
     1741 gaatccagac agccgatcgc tgacccgccg gcagttggcg tccggccttc tcgtcgccgg
     1801 cgcggcctgt ctgccggcga cggcgaacgc cggcccggcc ctcgacggag aggccgacgc
     1861 cgcgcttgcg cgcctctacg cggccaacgc caaggcccgc gaactgggcc aacgtgcccg
     1921 ggccgtcctg atcttcccca gtatcgccaa ggcgggcctt ctggtgggcg gccagaccgg
     1981 caagggcgtt ctccagaccg tcggcgagcc ggcgcgctac taccgcatca cggcggcgtc
     2041 ctacgggttg caggctggcg cccagaccta cagctacgcg ctcttcttca tgacccggtc
     2101 gtcgctcgac tacctgcatg tcagtgacgg atggtcgatc gggacggggc caagcgtcgt
     2161 gttcatcgac gagggcttcg cgcggacgct gaacacgaca acccttaccc aggacgtcta
     2221 cgccatcgcc ttcgggcaac ggggcctgat ggccggcacg gggctggagg gcgcgaagat
     2281 ctccccgatc tccgcgccct gacaagacgg ccgacgcctt gaggaattcg cttcgaagcc
     2341 ggctccggct tcgcgccgcg ttcgtgctcg ccatcctggc cgtgggcgcg agcgcgcgcg
     2401 gcgcggcaaa gccgagtgcg aggtgcgcct gctcgaagcg gcgcgggtcc gccgaaaggc
     2461 cgccgatgaa ggcc
//
LOCUS       DAORNX010000437         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32140_length_2463_cov_5.618771, whole genome shotgun sequence.
ACCESSION   DAORNX010000437 DAORNX010000000
VERSION     DAORNX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32140_length_2463_cov_5.618771"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..542)
                     /locus_tag="PLO65_14885"
     CDS             complement(75..542)
                     /locus_tag="PLO65_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRD29583.1"
                     /translation="MDWKTLFFSPDGRIGRSAFWIGWLVLLGVNVVLGWIPLIGWALS
                     LVTIYCNVCVVSKRLHDMGRSGFLQVWPIVLCTVLCVGALGLAAGPAIMAGISNADDA
                     AITAAVMAGVGGMLLAFLAAFVIGFGFLLWVGISNGEPGENRYGPAPRQPAIA"
     gene            621..1232
                     /locus_tag="PLO65_14890"
     CDS             621..1232
                     /locus_tag="PLO65_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HRD29584.1"
                     /translation="MSFHEVRLPARLAFGSTGGVERRTEIVTLASGFERRSTPWAHGR
                     RRYLIGAGLRSLDDAAALITFFEARRGQLYGFRFRDFADFKSCAPSAPVSPIDQMLGL
                     GDGETSEFLLTKAYGDLGRPIAKPVAGSVRVAIDGSETAAFTLDATTGRVTFTTPPGL
                     NAVLTAGFEFDTPVRFDADRIDVTLDSFAAGRVIAAPLIELRI"
     gene            1236..1895
                     /locus_tag="PLO65_14895"
     CDS             1236..1895
                     /locus_tag="PLO65_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HRD29585.1"
                     /translation="MKSIPAALADRIESGAATLAHVWLLTRPDGTRLGFTDHDEDLLH
                     DGVICRATSGWTAGASDLRSGFAPGGASVRGALDSEALAAADLDAGLYDGSAVVCRRV
                     DWREPTLAAPLWAGAITRVKREGDAFLAEIDGPLAALDRTAGRAFGRLCDANLGDHRC
                     AVAADHPAFAEGCDKRWRTCSDRFGNTLNFRGFPTIPGEDFLAVYPARGERHDGKSRR
                     G"
     gene            1888..2337
                     /locus_tag="PLO65_14900"
     CDS             1888..2337
                     /locus_tag="PLO65_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD29586.1"
                     /translation="MDRNAIVVAARGWIGTPYRHQASVKGAGCDCLGLVRGVWRELVG
                     EEPETPPPYRPDWAETGGRETLLEAARRHLVEAPVDRLRPGDVLLFRMSPGACLKHCA
                     ILSAARPEDPAPRMIHAYWGRAVVESWLESWWRRRLAHAFSFPNVSD"
BASE COUNT          357 a          886 c          860 g          360 t
ORIGIN      
        1 attcctggcc atcatcggcg ccgccgcggc caacgccagc taggcgtcag tcgcggccgc
       61 gcgccgggca gggatcaggc gatcgccggc tgccgcggcg ccgggccgta gcggttctcg
      121 cccggctcgc cgttcgagat gccgacccac agcaggaagc caaagccgat cacgaaggcg
      181 gccaggaagg ccagcagcat gccgccgacg cccgccatca ccgccgccgt gatcgccgca
      241 tcgtcggcgt tcgaaatgcc ggccatgatc gccggccccg ccgcgaggcc cagggcgccg
      301 acgcacagca ccgtgcacag cacgatcggc cagacctgca ggaagccgct gcggcccatg
      361 tcgtggagcc gcttcgaaac cacgcagacg ttgcagtaga tcgtcaccag gctcagggcc
      421 cagccgatca gcggaatcca gcccagcacc acgttgacgc ccaacaacac cagccagccg
      481 atccagaagg ccgaccggcc gatacggccg tccggcgaaa agaacagcgt cttccagtcc
      541 atgtgagtct ctccagccct ccccggaccg taaacccgtg ttcggcggaa gggtaggggc
      601 ggcccgatta gggatttgtc atgtcctttc atgaagtccg cctgccggcg cggctggcgt
      661 tcggttcgac cggcggggtc gagcgccgca ccgagatcgt caccctcgcc tccggcttcg
      721 agcggcggtc gacgccctgg gcccatggcc gacgacgcta tctgatcggc gccggccttc
      781 ggtcgctgga cgacgcggcg gcgctgatca ccttcttcga ggcccggcgg ggccagctgt
      841 acggctttcg tttccgcgac ttcgccgatt tcaagtcgtg cgccccgtcg gcgcccgtct
      901 cgcccatcga ccagatgctg ggcctgggcg atggggaaac gagcgaattc ctcctgacca
      961 aggcctacgg cgatctcggc cgcccgatcg ccaagccggt ggccggcagc gtccgcgtgg
     1021 ccatcgacgg cagcgagacg gcggccttca ccctcgacgc cacgacgggc cgggtgacct
     1081 tcaccacgcc gccggggctg aacgccgtgc tcacggccgg cttcgaattc gacacgcccg
     1141 tgcgcttcga cgccgaccgc atcgacgtga cgctggacag tttcgcggcc gggcgggtga
     1201 tcgccgcgcc gctgatcgag ctgcggatct gaacgatgaa atccatcccc gccgccctgg
     1261 ccgaccggat cgagtccggc gcggccaccc tggcccatgt ctggctgctg acccgcccgg
     1321 acgggacacg gctgggcttc accgatcatg acgaggacct gctccatgac ggggtgatct
     1381 gccgggcgac gagcggatgg accgccgggg ccagcgatct gagatcgggt ttcgcgcccg
     1441 gcggcgcgtc cgtccgcggg gcgctggaca gcgaggcgct ggcggcggcg gacctcgacg
     1501 ccggtctgta cgacggttcc gccgtggtct gtcgccgggt cgactggcgc gagccgacgc
     1561 tggcggcgcc gctgtgggcc ggggccatca cccgggtgaa acgcgagggc gacgccttcc
     1621 tggccgaaat cgacgggccg ctggcggcgc tcgacagaac cgccggccgc gccttcggcc
     1681 ggctgtgcga cgccaatctc ggcgatcacc ggtgcgccgt cgcggctgat catccggcct
     1741 tcgccgaggg ctgcgacaaa cgttggcgca cctgttcaga tcgcttcggt aacaccctga
     1801 acttccgggg gtttccaacc atcccagggg aagattttct ggccgtctat ccggcgcgcg
     1861 gagagcgcca tgacgggaaa tcccggcgtg gataggaacg ccatcgtcgt cgccgcgcgg
     1921 ggatggatcg gtacgcccta ccggcaccag gcttccgtga agggggccgg ctgcgactgt
     1981 ctgggcctgg tgcgaggggt ctggcgggag ctggtcgggg aggagcccga gacgccgccg
     2041 ccctatcgcc cggactgggc cgagaccggc gggcgcgaaa ccctgctcga ggccgcccgg
     2101 cggcatcttg tcgaggcgcc ggtcgaccgg ctccggcccg gggacgtgtt gctgttccgc
     2161 atgagtcccg gcgcctgcct caagcactgc gccatcctgt cggcagcccg gcctgaagat
     2221 cccgccccgc gcatgatcca cgcctactgg ggccgagcgg tcgtagagag ctggctggag
     2281 tcctggtggc ggcgtcggct ggcccacgcc ttttcctttc ccaatgtgag cgactgacat
     2341 ggcccaggtg atcctcggcg gcctcggccg cgcgctcgga ggcgggcttg gcgcggcgat
     2401 cggcggcctc gtcggcagcg cgctggaccg ggcggccctc tcggcgctcc aaccggccag
     2461 gca
//
LOCUS       DAORNX010000438         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32303_length_2453_cov_7.441618, whole genome shotgun sequence.
ACCESSION   DAORNX010000438 DAORNX010000000
VERSION     DAORNX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32303_length_2453_cov_7.441618"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..2377
                     /locus_tag="PLO65_14905"
     CDS             332..2377
                     /locus_tag="PLO65_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRD29587.1"
                     /translation="MKSSIDRRSYSVQGDLQATSGSIADALRRVPSVEVDMEGNVKLR
                     GDPGVTILIDGKPSSIMQGQGRADALQMLPADQIERVEVLTNPGAQYSPEGTGGVINL
                     VTRSSARAGAGASGSVRANVGTGDRFNGGINWAFNSKKLALSANASLMRFGNRFDGTD
                     DRSLTGPGGVYRQRQTSSTEMQGVGGNARIAADYDLDPHNRISGALNVFQFRPDFDSD
                     SLSETFDGAGSSLGVIRRKGSADGASLFASGSLSWRREFAGDDHTLTVELNRDRWAAD
                     NDSAFRVDTLSPAAPSQFEDSDGRIVASNLTFKGDYSRPMPRNGRLKTGFDVQNREND
                     SDTVLSVGPAEDATVIDPSRSHRLIFEETVYSGYLTYEQPFGDFTVLAGLRYEAATND
                     INLLTTGLTTRHDYDRAYPSLHLDYRLSDTSRLKASYALRIRRPGASDLDPWLVYIDA
                     YNYRQGNPDLEPSETRSWELSYEYRKGKTYYLATGFWRQTDNGVTDLLVDLGGGVLLT
                     TKANLAESRSGGVELVANSSLSPTLSLNLTGTALWNQIDAASLGYGGDRSAAALSGRA
                     SVDWQVTADDLVQVNLVVTGKSITPQGHIEPTYTTNLGYRRKIGDRWFLTVTATDLFD
                     TQETRLVTDSLAVNGVNVRKGASRGLFVGLRYRFGAGKPQRDPAFDYGGGGPGGPPS"
BASE COUNT          443 a          843 c          788 g          379 t
ORIGIN      
        1 cgaccgacgg cggcaagacc tgggcgacaa acttcgactt cacctacagg ccggccaccg
       61 gcgccacgcc gcccggcggc tgacgcgtgg gtggaccctg aaacggtctt tcttttgcct
      121 gcttgagcaa acacgcttag gtcgccgtat ggaagcgggg gccttcaggt tgcgtgacag
      181 aaacaggatc cccgcgcggg ttggcctgat ggccgcgttg atggcggcgt cggtgggcga
      241 agctcagacc gcgcccgcgc ccaaggcgcc gcccgccggt gatggctcaa ccacggtcca
      301 gggggtggtg gtcaaggcgg acgacacgcc aatgaaaagc tccatcgacc gccgcagcta
      361 cagcgtacag ggcgatcttc aggccaccag cggctcgatc gccgacgccc tgagacgcgt
      421 tccctcggtc gaggtcgaca tggagggcaa cgtcaaactg cgcggggacc ccggcgtcac
      481 catcctgatc gacggcaagc cgtcgagcat catgcagggt cagggccgcg ccgatgcgct
      541 gcagatgctt ccggccgacc agatcgagcg ggtcgaggtg ctgaccaatc ccggcgccca
      601 gtactcgccg gagggaaccg gcggcgtcat caatctggtg acccggagtt cggccagggc
      661 cggggccggc gcatccggct cagtgcgagc caatgtcggt acgggggacc gcttcaatgg
      721 gggaatcaac tgggctttca acagcaagaa actggccctg tcggcgaacg ccagcctgat
      781 gcgtttcggc aaccggttcg acgggaccga tgaccgctcg ctcaccggtc cgggcggtgt
      841 ctatcggcaa cggcagacga gctcgaccga gatgcaggga gtgggcggaa acgcccggat
      901 cgctgccgac tacgacctgg atccccacaa ccgcatcagc ggcgcgctca acgtcttcca
      961 attcaggccg gactttgaca gcgacagtct ctccgaaacc ttcgacggcg ccgggtcaag
     1021 cctgggggtc atccgccgga agggctcggc cgacggggcg tcgctcttcg cctccggctc
     1081 cctgagctgg cggcgggaat tcgcgggcga cgaccacact ctgacggtcg aactgaaccg
     1141 cgaccgctgg gctgcggaca atgactcggc cttccgcgtc gacaccctgt cgcccgccgc
     1201 gccgagccaa ttcgaggaca gcgacggccg gatcgtcgcc agtaacctca ccttcaaggg
     1261 tgactacagc cgccccatgc cgcggaacgg ccgcctcaag acgggctttg acgttcagaa
     1321 cagggagaac gactccgaca cagtgctttc ggtcggaccg gccgaggacg cgacggtgat
     1381 cgatcccagc cgctcgcacc gcctgatttt cgaggagacg gtctattctg gctatttgac
     1441 ctacgaacag ccgttcggcg atttcacggt cctggcaggc ctgcgctacg aggcggcgac
     1501 gaacgacatc aatctgctga ccacggggct gacgacccgc catgactacg accgggccta
     1561 tccctcgctg cacctcgact accggctcag cgacacgtcc cggctgaagg ccagctacgc
     1621 cctgcggatc cgtcgcccgg gcgccagcga cctcgatccc tggctggtct acatcgacgc
     1681 ctacaactat cggcagggca atcccgatct cgagccgtcg gagacccgtt cctgggagct
     1741 gtcctacgag tatcgcaagg gcaagaccta ctaccttgcg accggcttct ggcggcagac
     1801 ggacaatggg gtcaccgatc tgctggtcga tctgggcggc ggcgtcctgc tgaccacaaa
     1861 ggccaatctg gccgagagcc ggtcgggcgg cgtcgagctt gtcgccaaca gcagtctctc
     1921 gccaacgctt agcctcaacc tcacaggaac ggcgctgtgg aaccagattg acgccgcctc
     1981 gctgggctac ggcggcgacc gttccgccgc cgcgctcagc ggccgcgcca gtgtcgactg
     2041 gcaggtcacg gccgacgatc ttgttcaggt caatctggtg gtcaccggca agtcgatcac
     2101 accgcagggc cacatcgagc cgacctacac gaccaacctc ggctatcggc gcaagatcgg
     2161 cgaccgatgg ttcctcaccg tgaccgccac cgacctgttc gacacccagg agacccgcct
     2221 ggtcaccgac agtctggccg tgaacggcgt caatgtccgc aaaggggcca gccgcggcct
     2281 gttcgtcggc ctgcgctacc gctttggcgc gggcaagccc cagcgcgatc cggcctttga
     2341 ctacggcggc ggcggcccgg gtggcccgcc atcctaggcg atcgggctga cgacgaggaa
     2401 ctggggctct tcgacccagg ccatgaacgc gcccatcacc gtctggatca ccg
//
LOCUS       DAORNX010000439         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32304_length_2453_cov_6.627606, whole genome shotgun sequence.
ACCESSION   DAORNX010000439 DAORNX010000000
VERSION     DAORNX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32304_length_2453_cov_6.627606"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLO65_14910"
     CDS             <1..146
                     /locus_tag="PLO65_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29588.1"
                     /translation="WDDIPAAHEKMLDNQHLPGNMGVLVCAQRPGLRTFEEVQEISAE
                     MGC"
     gene            712..2091
                     /locus_tag="PLO65_14915"
     CDS             712..2091
                     /locus_tag="PLO65_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD29589.1"
                     /translation="MYFIAGGLALGVFAIGFLIAIVLRRVVPTNEVHIVQRGRTRVSY
                     GASQTAGNVYYEWPAFLPRIGVLVTKFPVSIFDVNLKDYEAYDTGRLPFRVDVQAFMR
                     IAKSDTAAEKVANFDELQHQLSGILQGAVRNVLATHKLEDILEARSTLAEAFTAQVDN
                     QLQAWGVETVKNIEFMDIRDSANSQVIANIMAKEKSRIDRESREAVAMNSQLAQTKEI
                     EAERIVEVNKQDALQQVGMRTAEQEKQVGIAKQVAEQEVLVAQKDTTEREMDVKLVAD
                     TRSADIAKGVAEVQAAQEREVTIIRAEGQRRQQVIAAEAERQRLELVAQGTLAQQKLD
                     AEGLLAQGQSRAEAERLLLLAPVSTQIELAKEIGGNDGYQTYLVRVEQIKAGQAVGVA
                     QAEALSAADIKVIVTGGDVPGGMNSITDVFSAKGGQALGAMLEGLAQTETGKAVVERA
                     TKGAAKSKE"
BASE COUNT          456 a          788 c          819 g          390 t
ORIGIN      
        1 gctgggacga catccccgcc gcccacgaaa agatgctcga taaccagcac ctgccgggca
       61 acatgggcgt gctggtctgc gcccagcgcc cgggcctgcg gacgttcgag gaggttcagg
      121 agatctcggc ggagatgggc tgctaggccc accaacgtca gaagcgcgtt tcaggcggcg
      181 gctctcgcga gggggccgcc gcctttgcgt ttgggggcgc gcgagtatgc ccgcgcctgc
      241 tttcgacgct tagcgaacct tcagagcgcc agctttcggc agtccccgcc cttcagcctt
      301 ggcggagcat tgcgcgggcg ggcgacttgc tcaaggaccg cttcgcggcc gcgcagcggc
      361 gggcccgcag ggcccgtcct tgaccaaggc gaccgctccg cgctcccatt ccatcaaggc
      421 tttggggcgg gtgtcatcag gcctgcggac cttcgaggag gttcaggaga tttcggggga
      481 gatgggtggc caggcttcag ggacagacac ccggacgtgt ctgtcccgat tgtgacgcgc
      541 ttcaggcggc ggctctcgcg agggggccgc cgcttttgct tttcggcggc tgcttgccag
      601 cgtccgccgc tactccaacc gttgaaccgg cggccccgca gccacccgcg atagttgcgc
      661 cacctggtgt tagcggacat agtccggccg acttcgcagg ggcacacaaa catgtatttc
      721 atcgctggcg gcttggcgct cggcgtcttc gccattggat tcctgatcgc gattgtgctt
      781 cgccgggtgg tcccgaccaa cgaagtgcac atcgtccaac gcgggcggac ccgcgtcagc
      841 tatggcgcca gtcagacagc cggaaacgtc tactatgagt ggccggcctt cctgccccgg
      901 atcggcgtcc tggtgaccaa gttcccggtc tccatttttg acgtgaacct gaaagactac
      961 gaagcctacg acaccggacg ccttccgttc cgcgtggacg tgcaggcgtt catgcggatc
     1021 gcgaagtcgg acacggcggc ggaaaaggtc gccaacttcg atgagctgca gcatcagctg
     1081 tccggcatcc ttcagggcgc cgtccggaac gtgctggcca cccacaagct cgaagacatc
     1141 ctcgaagccc gatcaacttt ggccgaggcc ttcaccgcgc aggtggacaa tcagcttcag
     1201 gcgtggggcg tcgagacggt caagaacatc gagttcatgg acatccgcga cagcgccaat
     1261 tcgcaggtta tcgccaacat catggcgaag gaaaagtccc gcatcgaccg cgaaagccgt
     1321 gaggccgtgg cgatgaacag ccagcttgcc cagaccaagg aaatcgaggc cgagcgcatc
     1381 gtcgaagtga acaagcagga cgctctgcag caggtcggga tgcggacggc cgagcaggaa
     1441 aagcaggtcg gcatcgccaa gcaggtcgcc gaacaggaag tcctggtggc gcagaaggac
     1501 accaccgaac gtgagatgga tgtgaagctg gtcgccgata cgcgctccgc agacatcgcc
     1561 aagggggtcg cggaagtcca ggccgcacag gagcgcgagg tcacgatcat ccgggcggag
     1621 gggcagcgcc gacagcaggt catcgccgcg gaggccgagc gtcaacggct ggagctggtc
     1681 gcgcagggaa cgctggcgca gcagaagctc gatgccgagg gactcctggc acagggccag
     1741 agccgcgccg aagccgagcg gctgctgctc ctcgcgccgg tgtccacgca gatcgaactg
     1801 gccaaggaaa tcggcggcaa cgacggctac cagacctacc tggtgcgggt ggagcagatc
     1861 aaagccgggc aggccgtggg tgtcgcccag gctgaagccc tcagcgcggc cgacatcaag
     1921 gtgatcgtga cgggcggcga cgtgcccggg ggcatgaaca gtatcaccga cgtgttcagc
     1981 gcgaagggcg gtcaagcgct cggcgcgatg ctggaagggc tggctcaaac agaaaccggc
     2041 aaggcggtgg tggagcgggc gaccaagggc gcggcgaagt ccaaggaata gcgctgttcc
     2101 gatccgttcg gcggccgaca gaaatgtcgg ttggccggac tgctctcaac gtcgactccc
     2161 tcccttggcg gaccgtccgc caatccgctt ccgagctcct gcaatccccg cccttttgcc
     2221 ttgcagggga catgtgccgc cttcggtgcg tggccccggc agcttcaagc ggcaggggac
     2281 acgcactgaa gtgcatgtcc ccgggtttca cgcttcccac tcatcttcac tttcgcgagg
     2341 acgagcgaat ctactggtgg tccgccttct tccccaccac cgtcaaaagc atcgccgccg
     2401 ccccggcgag cagcacgatc ggcacggtcg agagcaccac ctgccccggg ctg
//
LOCUS       DAORNX010000440         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32417_length_2445_cov_5.881172, whole genome shotgun sequence.
ACCESSION   DAORNX010000440 DAORNX010000000
VERSION     DAORNX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_2445_cov_5.881172"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLO65_14920"
     CDS             <1..886
                     /locus_tag="PLO65_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD29590.1"
                     /translation="RVETPTLRPDKRLAENPPVDLPALRLKDYVADIAALARALEAET
                     GVAPVLFGHSMGGLIVQKLLEAGVGRAGVLITPASPADARGQRALGQALTFANILLSG
                     DVGSRSHKIWRWGFSWGVLNRVPRARHAGIFAGTVYDSGGVYEDIAYPERDPARTAFV
                     DETRITAPILTIGGAKDRAVPIGDVRLAAEKYSRVGGDYLEYVDNGHWIVDEPGTDRV
                     IEDIATWLASRAITPAAPVTKKIAKAGADKPAGARKASPAPKAASRAPAKARPAGKAA
                     ATKAPSGAKARVRKPKST"
     gene            951..1706
                     /locus_tag="PLO65_14925"
     CDS             951..1706
                     /locus_tag="PLO65_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29591.1"
                     /translation="MPAPVIMVHGAFCAGWVFERFREPFEAAGHEVLTPDLPGHEPGG
                     SPANRSMSDYADHVARLCRQSGEPPVLIGHSMGGLVAHLAAARAPVSGLILLAPSPPW
                     GVPGVSMEEAISAVSLYALGPFWLQAIDPDYALAKRFSLDRMDKEDRKAAFARMTAES
                     GLALWQTLNWWLDPFMTTSAPSGRVTAPALVLAGGRDVIHPPATVRQTADRLSARFEV
                     LRDMSHWLPGEPGWDSVAALCLDWISEGRRVAA"
     gene            complement(1678..1935)
                     /locus_tag="PLO65_14930"
     CDS             complement(1678..1935)
                     /locus_tag="PLO65_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29592.1"
                     /translation="MAWTDVVGVAGVLMILAAYAAATLGRVDVKGLLSLSANFVGASL
                     ILLSLSVDFNLSAALMEGSWALVSLVGLARVLLRRRPAGLH"
     gene            complement(1943..>2445)
                     /locus_tag="PLO65_14935"
     CDS             complement(1943..>2445)
                     /locus_tag="PLO65_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29593.1"
                     /translation="VPEAPPAPPPEPTDVTSLAVINTIERVCSPMVRGTPIAQVAPAL
                     GFKKKRDVWVWVYQKGYQVSIQPSTTNPTVCTIDVNHPVESVAPAATLATDLHYWAQR
                     QGGGWFLARFDKYVTDMERTTRSWEKEGDTEGQALVLMTERKITGAPVNPKYDHTQVL
                     FSIRPN"
BASE COUNT          379 a          843 c          876 g          347 t
ORIGIN      
        1 gcgggtggag acccccaccc tgcgacccga caagcggctg gccgagaacc cgccggtcga
       61 tctgccggcc ctgcggctga aggattatgt cgccgacatc gccgcgctgg cccgcgcgct
      121 ggaagcggag accggggtcg cgccggtgct gttcggccac tccatgggcg ggctgatcgt
      181 tcagaagctg ctcgaggcgg gcgtgggccg cgccggcgtg ctcatcaccc ccgcctcgcc
      241 cgccgacgcc cggggccaga gagccctggg ccaggcgctg accttcgcca acatcctgct
      301 cagcggggac gttggatcaa ggagccacaa gatctggcgc tggggcttct cctggggcgt
      361 gctcaaccgc gtgccccggg ccaggcacgc gggcattttc gccggcacgg tctacgacag
      421 cggcggagtc tacgaggata tcgcctatcc cgagcgggac ccggcgcgca ccgccttcgt
      481 cgatgagacg cggatcaccg ccccgatcct gaccatcggc ggcgcgaagg atcgcgcggt
      541 gccgatcggc gatgtgcggc tcgccgccga gaagtattcc cgggtcggcg gggactatct
      601 cgaatacgtc gacaacggcc actggatcgt cgacgagccg ggaacggacc gggtgatcga
      661 ggatatcgcc acctggctgg cgtccagggc gatcacgccg gccgcgccgg tgacgaaaaa
      721 gatcgcgaaa gccggggcag acaagcctgc gggcgcgcgc aaggcgagcc ccgcgcccaa
      781 ggccgcctcc cgcgcgccgg cgaaggccag gccggccggg aaggccgccg cgacgaaagc
      841 gccttcaggg gcgaaggcgc gtgttcgcaa gccgaaatca acgtagggtg cccgggtccg
      901 gacaggctgg cccaaggcag gcccgccgcc gggcctgaaa aggggaactg atgcctgcac
      961 ccgtcatcat ggtgcacggc gccttctgcg ccggctgggt gttcgaacgc tttcgcgagc
     1021 cgttcgaggc cgccggccac gaggtgctga cgcccgacct gcccggacac gaacccggcg
     1081 ggtcaccggc caaccggtca atgagcgact atgccgacca tgtcgcgcgg ctgtgtcgcc
     1141 agagcggcga accgccggtc ctgatcggtc attccatggg cgggctggtg gcccatctcg
     1201 cggcagcccg ggccccggtg tccggtctga tcctgctggc cccgtcgccg ccctggggcg
     1261 tgccgggcgt gagcatggag gaggccattt cggcggtcag tctctacgcg ctcggtccct
     1321 tctggcttca ggcgatcgac cccgactacg ccctggccaa gcgcttcagc ctcgaccgga
     1381 tggacaagga ggaccgcaag gcggccttcg cgcgcatgac ggccgagagc ggccttgcgc
     1441 tctggcagac gctgaactgg tggctggatc cgttcatgac cacctccgcg ccctccggcc
     1501 gggtcacggc cccggccctg gtgctggcgg gcggccggga cgtgatccac ccgccggcga
     1561 cggtcagaca gaccgccgac cggctgtccg cgcgcttcga ggtgttgcgg gacatgagcc
     1621 actggctgcc cggcgagccg ggctgggact cggtggcggc cctgtgcctg gactggatca
     1681 gtgaaggccg gcgggtcgcc gcctgagcag cacccgggcc aggccgacca gcgacaccag
     1741 ggcccaggag ccctccatca gcgccgctga caggttgaag tcgaccgaaa gcgacaacag
     1801 gatcaacgac gcgccgacga aattggccga caaggacagc aggcccttga cgtcaacccg
     1861 gccgagcgtg gccgccgcgt aggcggccag gatcatcagg accccggcca cgccgacgac
     1921 atcggtccag gccacggcca gatcagttcg ggcggatgct gaacaggacc tgggtgtgat
     1981 cgtacttggg attcaccggc gcgccggtga tcttgcgctc ggtcatcagc accagggcct
     2041 gcccctcggt gtcgccttcc ttttcccagg agcgggtcgt gcgttccatg tcggtgacgt
     2101 acttgtcgaa ccgggccagg aaccagccgc cgccctgacg ctgcgcccag tagtgcaggt
     2161 cggtcgccag ggtcgcggcc ggcgcgacac tctcgaccgg gtggttcaca tcgatggtgc
     2221 agacggtcgg attggtcgtc gagggctgga tcgagacctg gtagcccttc tggtagaccc
     2281 acacccacac gtcgcgcttc ttcttgaagc cgagggcggg agccacctgg gcgatcggcg
     2341 tgccgcggac catcgggctg cagacacgct cgatggtgtt gatcacggcc agcgaagtga
     2401 cgtcggtcgg ctcgggcggg ggggccgggg gcgcttccgg caccg
//
LOCUS       DAORNX010000441         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32470_length_2442_cov_7.253875, whole genome shotgun sequence.
ACCESSION   DAORNX010000441 DAORNX010000000
VERSION     DAORNX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32470_length_2442_cov_7.253875"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..>2442
                     /locus_tag="PLO65_14940"
     CDS             409..>2442
                     /locus_tag="PLO65_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRD29594.1"
                     /translation="MADGPVIGRTIADSTPFWPDRPRPPKGAPNILVVLFDDVGFSDF
                     GCYGSPIETPTIDALAAGGLRYTGFHTTAMCSTTRAALLTGRNHHSVGVGCLANFDSG
                     FPGYRGKIAREAGTLPEMLKPHGYRSYMVGKWHVTPLTESGATGPFDGWPLGRGFDRF
                     YGFLDAETDQYSPELVRDNTPVAAPGTYETGYHLTEDLVDQAIRYIADHTADNPETPW
                     LTWLALGACHAPHQVPRDIIEKYDAVFSGGWDVERERRLARQKALGVVPADTRMPPRN
                     DWVKPWDEHTPDEQRLFTRLQAAFAGMLDHADRHLARLVAFLDEAGLREDTLILVLSD
                     NGASQEGGPLGMVNAMGPYNGKPEPMEEKIARIDDIGGPDTHSNFPLGWAMASNTPLR
                     RYKQNTHGGGIRDPLVISWPAGIPARGELRGQFTHACDVTPTILDILGLEPPAAVAGV
                     AQTPLEGSSFKASLFDGAAAAKAGPQHFEMFGHRGIWKDGWKAVAWHPPGRPFDADQW
                     ELYHLDQDFSETEDLALTHPDRLEAMIALWWAQAEAHKVLPLDDRFAERFAVNAQRFH
                     GARKRFVFHRGMGHVPTDVAPDIRSRSYFIEAEARLDGEDEGVLIAHGDATCGYSLFV
                     RDGRLVHDMNIGGRHVTLTSDRPVEPGHRRLGVAVRPGPGGRRIVLMIDGVAAG"
BASE COUNT          373 a          863 c          873 g          333 t
ORIGIN      
        1 ggtctggttg cgcgcgagac cctcgccgcg cggcgcccag aggaaccggc cggcgatctc
       61 ctcccccgcc gtgcgacggt gactgaccca ccaatggcgc atgagggggc tccgggtgtc
      121 cgccactata cttccggttg cgttccggcc aaccccctcc tgttctccat catcgtcgga
      181 cttgctccga cgatgatgga ggaatggcgg gccagcgcgt cgggcggatt gcgtggttcg
      241 acacgcgctc ttcgcgcgct gctcaccatg acgtggaggg gcgctgacgc acgtcatcct
      301 gagcagggcc gaaggcccgt gtcgaaggac gcacctgggc cccgttaccc actggtaacg
      361 cccggcggcg ccgcctatcc tgccgcgaaa cacacaggga gacgcgccat ggctgacgga
      421 ccggtcatcg ggcggacgat cgccgattcc acgccgttct ggcccgaccg gcccaggccg
      481 ccgaagggcg cgccgaacat cctggtggtg ctgttcgacg acgtcgggtt cagcgacttc
      541 ggctgctacg gctcgccgat cgagacgccg accatcgacg ccctggccgc cggagggctg
      601 cgctacacgg ggtttcacac cacggcgatg tgctcgacca cccgggcggc gctgctgacc
      661 gggcgcaacc accacagcgt cggcgtcggc tgcctggcca acttcgacag cggctttccg
      721 ggctatcgcg gcaagatcgc gcgggaggcc gggaccctgc cggagatgct caaaccgcac
      781 ggctaccgca gctacatggt cggcaagtgg catgtgacgc cgctgaccga gagcggcgcg
      841 accgggccgt tcgacggctg gccgctgggg cgcgggttcg accgcttcta cggcttcctc
      901 gacgccgaga ccgaccagta ttcgcccgag ctggtgcgcg acaacacgcc ggtcgccgcg
      961 ccggggacgt acgagaccgg ctatcacctg accgaggacc tggtcgacca ggcgatccgc
     1021 tacatcgccg accacacggc cgacaatccc gagacgccgt ggctgacctg gctggcgctg
     1081 ggcgcctgcc acgcgccgca ccaggttccg cgcgacatca tcgaaaaata cgacgctgta
     1141 ttttctggcg gctgggacgt cgagcgcgag cggcggctgg ccaggcagaa ggcgctgggc
     1201 gtcgtgccgg cggacacgcg gatgccgccg cgcaacgact gggtcaagcc gtgggacgag
     1261 catacgcccg acgagcagcg gctgttcacc cggctgcagg cggcgttcgc gggcatgctc
     1321 gaccacgccg accggcacct ggcgcggctg gtggccttcc tcgacgaggc cggcctgcgc
     1381 gaggacaccc tgatcctggt gctgtcggac aacggggcca gccaggaggg cgggccgctg
     1441 ggcatggtca acgccatggg cccctacaac ggcaagcccg agccgatgga ggagaagatc
     1501 gcccgcatcg acgacatcgg cgggccggat acgcattcga acttcccgct cggctgggcg
     1561 atggcctcga acaccccgct gcggcgctac aagcagaaca cccacggcgg cggcattcgc
     1621 gatccgctgg tgatcagctg gccggcgggg atcccggcgc gcggcgagct gcgcgggcag
     1681 ttcacccacg cctgcgacgt gacgccgacg atcctcgaca tcctcggcct ggagccgccc
     1741 gcggccgtcg ccggcgtggc gcagacgccg ctggagggga gcagcttcaa ggcctcgctg
     1801 ttcgacggcg ccgccgcggc caaggccggg ccgcagcact tcgagatgtt cggccatcgc
     1861 gggatctgga aggacggctg gaaggccgtc gcctggcacc cgccgggccg gccgttcgac
     1921 gccgaccagt gggagctcta ccacctggac caggacttct cggagaccga ggacctggcc
     1981 ctgacccatc cggaccggct ggaggcgatg atcgccctgt ggtgggccca ggccgaggcg
     2041 cacaaggtgc tgccgctcga cgaccgcttc gccgagcggt tcgcggtcaa cgcccagcgc
     2101 ttccacggcg cgcgcaagcg gttcgtgttc catcgcggca tgggccatgt gccgaccgac
     2161 gtggcgcccg acatccgcag ccgcagctac ttcatcgaag ccgaggcgcg gctcgacggc
     2221 gaggacgagg gggtgctgat cgcgcacggc gacgcgacct gcggctacag cctgttcgtg
     2281 cgcgacgggc ggctggtgca tgacatgaac atcggcggcc ggcatgtgac gctgaccagc
     2341 gaccggccgg tcgagcccgg ccacaggcgg ctgggcgtgg ccgtgcggcc gggcccgggc
     2401 gggcggcgca tcgtgctgat gatcgacggc gtcgcggccg gc
//
LOCUS       DAORNX010000442         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32612_length_2433_cov_6.440286, whole genome shotgun sequence.
ACCESSION   DAORNX010000442 DAORNX010000000
VERSION     DAORNX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32612_length_2433_cov_6.440286"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLO65_14945"
     CDS             <1..258
                     /locus_tag="PLO65_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRD29595.1"
                     /translation="NALDKLIGGAARTGLDPSSGFVLVTSRCSYEMVEKAAVAGFPLL
                     VAISAPTALAIRKAQECGMTLVALARADGCAVFTGPERIAP"
     gene            255..>2433
                     /locus_tag="PLO65_14950"
     CDS             255..>2433
                     /locus_tag="PLO65_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HRD29596.1"
                     /translation="MSRARGVTPYRRPAGGWGALRAVADALADQETVLEGGRTLLSAN
                     QPDGFDCPGCAWPDPKHTSSFEFCENGAKAVAWEATGKRATPEAIAASTVSQLRAWSD
                     HALEDLGRLTEPLRYDGATDRYERVGWDEATARIGTAMAALPDPDMAAFYASGRASNE
                     AAFLFQLWGRRFGTNNFPDCSNMCHEPTSVGLAEQLGLGKGSVTLEDFDHCDAVFSFG
                     HNPGTNHPRMMATLREVARRGAPIVVFNPLRERALEKFASPQDPVEMATLGGTDIAHR
                     YYQPRIGGDVAAVEGMMKTLIARGQVDRPFIDQHTAGYEALVAHLAGRDWADLEARSG
                     LTRGQIEEAAAIFAASRAVILCYGMGVTQHRSASSAVQQLVNLLLLGGHIGRPGAGVC
                     PLRGHSNVQGARTVGLTEKPSAALLASLEAAFGFTPPARHGHTVVEAIAAMRAGTLRA
                     FVGLGGNFAVAAPDPAVTFPALQALDLTVHIATKLNRTHLLPGRDSYILPCLGRTEID
                     IQAGGRQSVTVEDSMSMVHASRGLNPPASEHLRSEPWIVAALASASLGPDSGIDWDGL
                     VADYDLIRDRIEAVFPVTFAGYNRRVRVPGGFRLPVPASHRVWETASGRADLIPFRHD
                     DPARPDSARGDPEVLILTTLRSHDQYNTTVYGLDDRYRGVKGRRDVVFAHPDDLAARG
                     LADGDRVDLTAAFPEDGADRSVRGFTAVARDLPRGCLAAYYPEA"
BASE COUNT          328 a          898 c          858 g          349 t
ORIGIN      
        1 aacgcgctcg acaagctgat cggcggcgcg gcgcggaccg gcctcgaccc gtcgagcggc
       61 ttcgtcctcg tcaccagccg ctgttcctac gagatggtcg agaaggccgc cgtggccggt
      121 ttcccgctgc tggtggccat ctcggccccc accgccctgg ccatccgcaa ggcgcaggag
      181 tgcggcatga ccctggtcgc cctggcccgg gccgacggct gcgcggtgtt caccgggccc
      241 gaaaggatcg cgccatgagc cgcgcccggg gcgtgacgcc ctatcgccgt ccggccggcg
      301 gctggggggc cctgcgggcg gtagccgacg cccttgccga ccaggagacg gtgctcgagg
      361 ggggccgcac cctgctgtcg gccaaccagc cggacggctt cgactgtccg gggtgcgcct
      421 ggccggatcc gaagcacacc tcgtcgttcg agttctgcga gaacggggcc aaggccgtgg
      481 cctgggaggc gaccggcaag cgggcgacac cggaagccat cgccgcctcg acggtcagcc
      541 agctgagggc ctggagcgac cacgcgctgg aggaccttgg ccggctgacc gagccgctgc
      601 gctacgacgg cgcgaccgac cgctacgagc gtgtcggctg ggacgaggcg acggccagga
      661 tcgggacggc gatggcggcc ctgcccgatc cggacatggc cgccttctac gcctcgggcc
      721 gggcctcgaa cgaggcggcc ttcctgttcc agctgtgggg ccggcggttc ggcaccaaca
      781 acttccccga ctgctcgaac atgtgccacg agccgaccag cgtgggtctg gccgagcagc
      841 tgggactggg caagggttcg gtgacgctgg aggacttcga ccactgcgac gcggtgttca
      901 gcttcggcca caaccccggc accaaccatc cgcggatgat ggcgaccctg cgcgaggtgg
      961 cccggcgcgg cgcgccgatc gtggtgttca atccgctgcg cgagcgggcg ctggagaaat
     1021 tcgcctcgcc gcaggatccg gtggagatgg ccaccctcgg cgggaccgac atcgcccatc
     1081 gctactacca gccgcgcatc ggcggcgacg tggcggccgt cgagggcatg atgaagacgc
     1141 tgatcgcgcg cggtcaggtc gaccggccgt tcattgatca gcacacggcc ggatacgagg
     1201 ccctggtcgc ccatctggcc ggccgcgact gggcggacct cgaggcgcgg agcggcctga
     1261 cccgcggcca gatcgaggag gcggcggcca tcttcgcggc gagcagggcg gtgatcctct
     1321 gctacggcat gggcgtgacc cagcatcgct cggcctcgag cgcggtgcag cagctggtca
     1381 acctgctgct gctcggcggg catatcggcc ggcccggcgc cggcgtctgt ccgttgcgcg
     1441 gccattccaa cgtccagggc gcgcgcaccg tgggcctgac cgagaagccg tcggccgcgc
     1501 tgctggccag cctcgaggcg gcgttcggct tcacgccgcc ggcccgccat ggccatactg
     1561 ttgtcgaggc catcgccgcg atgcgggcgg ggaccctgcg ggcgtttgtc ggcctgggcg
     1621 gcaacttcgc cgtcgccgcc cccgatccgg ccgtaacctt ccccgcgctg caggccctgg
     1681 accttaccgt ccatatcgcc accaagctca accgcaccca cctgctgccc ggccgggaca
     1741 gctacatcct gccttgcctc ggccgcacgg agatcgacat ccaggccggt ggtcgacagt
     1801 cggtcacggt cgaggactcg atgtcgatgg tccacgcctc ccgcggcctc aacccgccgg
     1861 cctcggagca tctgcggtcc gaaccgtgga tcgtggcggc cctggccagc gcgtcgctcg
     1921 ggccggacag cgggatcgac tgggacggcc tggtcgccga ctacgatctt atccgcgacc
     1981 ggatcgaggc ggtctttccc gtcaccttcg ccggctacaa tcgccgggtc cgcgttccgg
     2041 gcggcttccg cctgcccgtt cccgcgtcgc accgcgtctg ggagacggcc agcggccggg
     2101 ctgacctgat ccccttccgc cacgacgatc cggccaggcc cgactcggcc cgcggcgatc
     2161 cggaggtgct gatcctgacc accctgcgca gccacgacca gtacaacacc accgtctacg
     2221 gcctggacga tcgctatcgg ggggtgaagg ggcgccgcga cgtggtcttc gcccaccctg
     2281 acgatctcgc ggcccggggc ctggccgacg gcgaccgcgt cgacctgacg gccgcctttc
     2341 ccgaggacgg ggccgaccgg tcggtgcgcg ggttcaccgc cgtggcccgc gacctgccgc
     2401 gcggttgcct cgccgcctac tatcccgagg cca
//
LOCUS       DAORNX010000443         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32690_length_2429_cov_5.184078, whole genome shotgun sequence.
ACCESSION   DAORNX010000443 DAORNX010000000
VERSION     DAORNX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32690_length_2429_cov_5.184078"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLO65_14955"
     CDS             <1..192
                     /locus_tag="PLO65_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29597.1"
                     /translation="GVARALAGAMMQQGFQAAPLLTLNARASAAAAPFWAAMGFEPVS
                     WPGITHALRLQWPGLSSPM"
     gene            complement(210..1157)
                     /locus_tag="PLO65_14960"
     CDS             complement(210..1157)
                     /locus_tag="PLO65_14960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRD29598.1"
                     /translation="MTRGRILVIKLSALGDFVQATGPMKAIRQAHPDAHITLLTTAPY
                     VALGEATGWFDAVWSDGRPDWTDLTTVWRLLRRIRAGRFDRVYDLQTQSRTVRYFQLL
                     WPSRPEWSGNAPGAALAYRGADRDRLHIQDSQREQLRIAGVSPVGPPDVSWLKGDADR
                     FGFQGPYALLVPGGAPHRPAKRWPAEKFGQLASALMAHGLRPVVLGHGPEEAALAGLI
                     RVIEPPTRSLVGETSLGDIASLARGAAVAIGNDTGPMHVAVAAGCPAVVLFSRESNPD
                     LSAPRRIRDDQPVTILREPDLADLAVDAVWAAVEAATSR"
     gene            complement(1169..2179)
                     /locus_tag="PLO65_14965"
     CDS             complement(1169..2179)
                     /locus_tag="PLO65_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD29599.1"
                     /translation="MDAANAAVARPAGSVDGLVALASHDMRGVNALISARMQSPVSVI
                     PALADHLINAGGKRLRPLLTVASARLVGAVDDSCLKLAAAVEFIHTATLLHDDVIDSS
                     QLRRGKVAAHLIWGAPSSVLVGDYLFARAFELMVEAGSLTALDILAKASGVISEGEVL
                     QLTRAHDLNLSQDVYLEIIRAKTAELFAAAAEAGAVSAGASDDLQRSMRAYGLNLGLA
                     FQLVDDALDYGGTTEALGKNAGDDFREGKATLPLLLAIARTGAAEAAFWERTIGRREQ
                     SDDDFRRARELIDRSGAVEATLDLAHRFAEEAKVALARTDANAWRAALEDLADFAVSR
                     RT"
     gene            2276..>2429
                     /locus_tag="PLO65_14970"
     CDS             2276..>2429
                     /locus_tag="PLO65_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD29600.1"
                     /translation="MADQGDVTEDRLLDGRVSLRQPARGYRAGMDAALLAAACDAAPG
                     ARVLDVG"
BASE COUNT          333 a          865 c          894 g          337 t
ORIGIN      
        1 ggggtggcgc gggcgctggc cggggcgatg atgcagcagg gcttccaggc cgcgccgttg
       61 ctgacgctga acgcgcgggc ctcggccgcg gcggcgccgt tctgggcggc gatggggttc
      121 gagccggtga gctggcccgg gattactcac gcgcttcgat tgcagtggcc tggtttgtcc
      181 tcgccgatgt agcgtccgcc catggctgtt cagcggcttg tggccgcttc caccgccgcc
      241 cagaccgcgt ccacggccag gtccgccagg tcgggctcgc gcaggatcgt caccggctgg
      301 tcgtcgcgga tccggcgcgg ggcgctgagg tcgggattgc tctcgcgcga gaacagcacc
      361 acggccgggc aaccggcggc caccgcgacg tgcatggggc cggtgtcatt gccgatcgcc
      421 acggcggcgc cgcgggccag gctggcgatg tcacccagcg aggtctctcc caccaggctg
      481 cgggtcgggg gttcgatcac ccggatcaga ccggccagcg ccgcctcctc cggtccatgc
      541 cccaggacca cggggcgcaa gccatgggcc atcagggcgc tggccagctg gccgaacttc
      601 tcggccggcc agcgcttggc cggccggtgc ggcgccccgc cggggaccag cagcgcgtag
      661 ggcccctgga atccgaacct gtcggcgtcc cccttcagcc agctgacatc gggcggcccg
      721 accggcgaga cgccggcgat gcgcagctgt tcacgctggg aatcctggat atgcagccgg
      781 tcgcggtccg ccccccggta ggccagggcc gcgccaggcg cgttgccgga ccattcgggc
      841 cggctcggcc agagcagctg gaaatagcgc accgtgcggc tctgggtctg caggtcatag
      901 acgcgatcga accggcccgc ccggatgcgc cggagcagtc gccacacggt ggtcaggtcg
      961 gtccagtccg gccggccatc gctccagacc gcgtcgaacc agcccgtcgc ctcgcccagg
     1021 gcgacatagg gggcggtcgt cagcagggtg atgtgggcgt ccggatgggc ctggcggatc
     1081 gccttcatcg gcccggtggc ctggacgaaa tcgccgaggg ccgacagctt gatgaccagg
     1141 atccgcccgc gcgtcacggc gcgatcgctc aggtccgccg gctgacggcg aagtcggcca
     1201 gatcttccag cgcggcgcgc caggcgttgg cgtccgtccg cgccagggcg accttggcct
     1261 cctcggcgaa gcggtgggcc agatccagcg tcgcctccac cgcgccggag cggtcgatca
     1321 gctcgcgcgc ccggcggaag tcgtcatcgc tctgctcgcg ccgcccgatg gtgcgttccc
     1381 agaaggcggc ctcggccgcg cccgtgcggg cgatggccag cagcagcggc agggtcgcct
     1441 tgccttcgcg gaagtcgtcg ccggcgttct tgccgagcgc ctcggtcgtg ccgccgtagt
     1501 ccagcgcatc atcgaccagc tggaaggcca ggcccaggtt caggccatag gcgcgcatcg
     1561 accgctgcag gtcgtcggag gcgcccgcgc tgacggcgcc ggcctcggcg gcggcggcga
     1621 acagctcggc ggtcttggcg cggatgatct cgagatacac gtcctgcgac aggttgagat
     1681 cgtgggcgcg cgtcagctgc agcacctcgc cctcggaaat gacgccggac gccttggcga
     1741 ggatatcgag cgcggtcagg gagccggcct cgaccatcag ctcgaacgcc cgcgcgaaca
     1801 ggtagtcgcc caccagcacg ctcgacggcg cgccccagat caggtgcgcc gccaccttgc
     1861 cgcgccgcag ctgcgaggaa tcgatcacgt cgtcatgcag cagggtggcg gtgtggatga
     1921 actccaccgc cgccgccagc ttgaggcagg aatcgtcgac cgccccgacc agacgggcgc
     1981 tggccacggt caacagcggc ctcagccgct tgccgccggc gttgatcagg tgatcggcca
     2041 gggccgggat gaccgagacc ggactctgca tgcgcgctga gatcagggcg ttgacgcccc
     2101 gcatgtcgtg ggacgccagc gccaccagtc cgtcgacgga acccgccggg cgggctacgg
     2161 ccgcattcgc tgcgtccaac acctgcgctc cttcatcccc gcgtcgcggc ccagcgccgt
     2221 tgcgggaccc atccccggcg ggataggtca ggcagcggac gagggcaagg ccgagatggc
     2281 ggaccagggt gacgtgaccg aggatcggct gctggacgga cgcgtcagtc tgcgccagcc
     2341 ggcccgtgga tatcgcgccg gaatggacgc ggcgctgctc gcggcggcct gcgacgcagc
     2401 gcccggcgcc cgggtgctgg acgtcggtt
//
LOCUS       DAORNX010000444         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32901_length_2418_cov_7.869234, whole genome shotgun sequence.
ACCESSION   DAORNX010000444 DAORNX010000000
VERSION     DAORNX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32901_length_2418_cov_7.869234"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /gene="cpaB"
                     /locus_tag="PLO65_14975"
     CDS             <1..>321
                     /gene="cpaB"
                     /locus_tag="PLO65_14975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRD29601.1"
                     /translation="AAAGDLPAAALTPVVAAAGAVPFGHKLEARNLTVIRMPASAVPE
                     GAYSSIQQVIGLDGGAPVTLTALVNHEPILPAKVSGGGARPSVAATISPGMRAYTIKV
                     DDVAG"
     assembly_gap    322..379
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <380..833
                     /locus_tag="PLO65_14980"
     CDS             <380..833
                     /locus_tag="PLO65_14980"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HRD29602.1"
                     /translation="VRSGAVEGASTTKNVIATLVIQNVRVLGMDLVADPASTEKSPPK
                     TATLEVTALDAGKLSAAGQAGTLSLALRRSGSTDVAQLRPMTLPELLRTDDGTGQSPV
                     LGPAIPVRRTVARHRAPSFPAAPAARGLVITQGGKRETVSVPSERFGS"
     gene            838..2187
                     /locus_tag="PLO65_14985"
     CDS             838..2187
                     /locus_tag="PLO65_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRD29603.1"
                     /translation="MRRIAIVCAAALACAPVAPPARAQAFVATSLVGELAPSRIITVP
                     KDKSVAFRLMGAAGEIVVAQSDVAEIVANTDRSVYVRGKALGTTNILVYDAGHRLMEV
                     IDVRVAQDIESLQADLGAALPGESIRVIPMAGGVLLSGRVSTSSVAARAKAIAERYAP
                     SGVTSDLTVEAAQQVMLEVRIIEASRSSLKDFGINLDIQNNSGIVFGSGTGLIGLNAP
                     QGSLTLSTNVGPTTIEATLRALEEKGVIRTLARPNLAAISGQEATFLAGGEFPYPVPT
                     GQGDITIEFKPFGVNLKFTPVVQDGGLINLKVAPEVSQLDYRQSLRLSNVEVPSILVR
                     RAATTIELKDGETFAIAGLFQQDYANTARQIPWAGDVPVLGALFRSSRWKKQETELLI
                     LVTPRLVTASESRALAPDPFKGSVEPSAIDLILNGLAMDRAMTAPGPSWPEPAPTGS"
     gene            2197..>2418
                     /locus_tag="PLO65_14990"
     CDS             2197..>2418
                     /locus_tag="PLO65_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29604.1"
                     /translation="MSFSGRRVLIIGAGLSDLAARALGDAALEAVAAESLNGRALQAD
                     PPDLVLIDAGGAATEVLAGAIAILGRAPTP"
BASE COUNT          339 a          882 c          799 g          340 t
ORIGIN      
        1 cgccgccgcc ggggatctgc cggccgccgc gctgacgccg gtggtcgcgg ccgccggggc
       61 ggtgccgttc ggccacaagc tcgaggccag gaacctcacg gtcatcagaa tgccggcctc
      121 ggccgtgccc gagggcgcct acagttcgat ccagcaggtg atcggactgg atggtggggc
      181 gccggtgacc ctgaccgcgc tggtcaacca cgagccgatc ctgcccgcca aggtcagcgg
      241 cggcggcgcc cggccgtcgg tggccgcgac catctcgccc ggcatgcgcg cctacaccat
      301 caaggtcgat gacgtggcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnc gtccggtccg gcgccgtcga gggcgccagc acgaccaaga
      421 acgtcatcgc caccctggtg atccagaacg tccgcgtcct gggcatggac ctggtggccg
      481 atccggcctc gaccgagaaa tcaccgccca agaccgccac cctggaagtc acggcgctcg
      541 acgccggcaa gctgtccgcg gccggccagg cgggcaccct gtcgctggcc ttgcgccgca
      601 gcggctcgac cgacgtggcg cagctgcgac cgatgaccct gccggagctg ctgcgcacgg
      661 acgacggaac cggccagtcc ccggttctcg gtccggccat tcccgtccgc cgcaccgtgg
      721 cccgccatcg cgcgccatcg ttcccggccg cgcccgcggc gcgcggcctg gtgatcaccc
      781 agggcggcaa gcgggaaacc gtctccgtgc cttcagagcg cttcgggagc tgacctcatg
      841 cgtcgcatcg ccatcgtctg cgcggcggct ctcgcctgcg cgcccgtcgc tccgcccgcc
      901 cgggcccagg ccttcgtcgc cacctcgctg gttggtgaac tggcccccag ccggatcatc
      961 accgtgccga aggacaagtc cgtcgccttc cggctgatgg gcgcggccgg cgagatcgtc
     1021 gtggcccagt ccgacgtcgc cgagatcgtc gccaacaccg accgcagcgt ctacgtgcgc
     1081 ggcaaggcgc tggggacgac caacatcctc gtctacgacg ccggccaccg gctgatggag
     1141 gtgatcgacg tccgggtcgc ccaggacatc gagtcgctgc aggccgacct cggcgccgcc
     1201 ctgccgggcg agtcgatccg ggtgatcccc atggccgggg gcgtgcttct gtcgggtcgc
     1261 gtctcgacca gttcggtggc cgcccgcgcc aaggccatcg ccgagcgcta cgccccgtcc
     1321 ggcgtgacct cggatctgac ggtcgaggcc gcccagcagg tgatgctgga ggtgcggatc
     1381 atcgaggcca gccgcagttc gctgaaggac ttcggcatca atctcgacat ccagaacaac
     1441 agcgggatcg tgttcggctc cggcaccggc ctgattggcc tgaacgcgcc ccaggggtcc
     1501 ctgaccctgt cgaccaatgt cggccccacc accatcgagg ccaccctgcg ggccttggag
     1561 gaaaagggtg tcatccgcac tctggcccgg cccaacctgg cggccatctc gggccaggaa
     1621 gccaccttcc tggctggcgg cgagttcccc tatccggtgc cgaccggcca gggcgacatc
     1681 accatcgagt tcaagccgtt cggggtgaac ctgaagttca cgccggtcgt ccaggacggc
     1741 gggctgatca atctgaaggt cgcgcccgag gtcagccagc tcgactaccg ccagagcctg
     1801 cgcctttcca acgtcgaggt gccgagcatt ctcgtccgtc gcgcggccac caccatcgag
     1861 ctgaaggacg gcgagacctt cgccatcgcc ggcctgttcc agcaggacta cgccaacacc
     1921 gcccgccaga ttccctgggc cggcgacgtg ccggtgctgg gcgccctgtt ccgctcgtcg
     1981 cgctggaaga agcaggagac cgagctgctg atcctggtca ccccccggct ggtgacggcg
     2041 tcggagagcc gggccctggc ccccgacccc ttcaagggct cggtcgagcc cagcgccatc
     2101 gacctgatcc tcaatggcct ggcgatggac cgcgcgatga ccgcgccggg cccttcctgg
     2161 cccgagcccg cgccgaccgg atcctgaggt gtcgagatga gcttttccgg acgacgtgta
     2221 ctgatcatcg gcgcgggact gtccgatctg gcggcgcggg cgcttggcga tgcggcgctc
     2281 gaagccgtcg ccgccgagag cctcaacggg cgggcgctgc aggccgaccc gccggacctg
     2341 gtgctgatcg acgcgggcgg ggccgcgacc gaggtgctcg ccggagccat cgccatcctc
     2401 ggccgggcgc cgacaccg
//
LOCUS       DAORNX010000445         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32941_length_2416_cov_8.272766, whole genome shotgun sequence.
ACCESSION   DAORNX010000445 DAORNX010000000
VERSION     DAORNX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32941_length_2416_cov_8.272766"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLO65_14995"
     CDS             complement(<1..989)
                     /locus_tag="PLO65_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRD29605.1"
                     /translation="MTFKRLTPAARPVLLDAAQFESRVRAHESFVAGRPDGRRALLRY
                     AVARALRCDRRKLPDADFTAADLSGTTFVGADLSRSSFYCANLTRCDFRGATLLRSDL
                     RGATFAGAKLGGANLDQADMRAAILCVADEALGLRWVGGQAQVAGSRLNGADLTDAVA
                     FAVDFSNCSLKGAMLRNANLKNANFTDANLDGVDLAGAKLAGVTLTGAILTNVDTGLL
                     QRSGVQLAGCVLDPTGEAIGRRDVIAAEIDRSHRWIESNGDVGQPAGLDDLDLRPAGQ
                     LFRNRLLGGMNARRTIGVGVDFSGAQLQGAVFDDADLRAANFTGADLRGVSFLR"
     gene            complement(1078..1884)
                     /locus_tag="PLO65_15000"
     CDS             complement(1078..1884)
                     /locus_tag="PLO65_15000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD29606.1"
                     /translation="MRRGPSWFNLVSLTVGFAFLYLPIVLLVVWSFNASRLVTVWGGF
                     SVRWYVALMKDHQLLDAAWTTLRIGLLSATAATVLGSLAAVALVRGGRFRGRTLFSGM
                     VYAPLVMPEVIMGLSLLLLFVALRFDRGFWTVMLAHTTFTLSYVAVVVHSRLITFDRS
                     LEEAAQDLGCTPSQAFLKVTLPNIFPAVLAGWMLAFTLSLDDLVIASFTSGPGATTLP
                     MRIYSQVRLGVTPEINAVCTILIAVVALGVIAWSLAQKWRAAGLAKLAAT"
     gene            complement(1881..>2416)
                     /locus_tag="PLO65_15005"
     CDS             complement(1881..>2416)
                     /locus_tag="PLO65_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD29607.1"
                     /translation="VILPFWTSFLIRVYAWVGILKPQGLLNAFLGLFGLGPLDILYTP
                     AAVQIGLVYSYLPFMVLPLYAALERLDDTLLEAAADLGSPPWKSFWQVTWPLSIPGVI
                     AGCLLCFIPMVGEFVIPDLLGGPGSLMIGRSIWMEFFANRDWPFASAVAIVLLVTLIA
                     PIVLYQRQQQKALEAGS"
BASE COUNT          443 a          820 c          848 g          305 t
ORIGIN      
        1 cggaggaagg acacccctct caggtcggct cccgtgaagt tggcggcccg caggtccgcg
       61 tcgtcgaaca ccgcgccctg cagctgcgcg cccgagaagt cgacgccgac cccgatcgtc
      121 cggcgggcgt tcatgccgcc cagcagccgg ttgcggaaca gctggccggc gggacgcagg
      181 tccaggtcgt cgagccccgc cggctggccg acgtcgccgt tgctctcgat ccagcggtgc
      241 gaccggtcga tctcggcggc gatgacgtca cgacgaccga tcgcctcgcc ggtggggtcg
      301 agcacgcagc cggccagctg cacgcccgat cgctgcagca gcccggtgtc gacattggtc
      361 aggatcgcgc ccgtcagggt gacccccgcg agcttcgccc cggccaggtc gaccccgtcg
      421 aggttggcgt cggtgaagtt ggcgttcttg aggttggcgt tgcgcagcat ggcgcccttc
      481 agcgagcagt tgctgaagtc cacggcgaag gccaccgcgt cggtgagatc ggcgccgttg
      541 agccggctcc cggcgacctg cgcctgtccg cccacccagc gcaggcccag ggcctcgtcg
      601 gcgacgcaca ggatggccgc ccgcatgtcg gcctggtcga gattggcccc gccgagcttg
      661 gccccggcga aggtcgcccc gcgcaggtcc gatcgcagca gggtcgcgcc acgaaagtcg
      721 caacgggtca ggttggcgca atagaacgag gagcggctca gatcggcccc gacgaaggtc
      781 gtgccgctca ggtcggcggc ggtgaagtcc gcgtcgggca acttgcgccg gtcgcaacgc
      841 agggcccgcg ccacggcata gcgcaacagg gcgcgccgac cgtccggccg tcccgccacg
      901 aacgactcat gggcgcggac gcggctctcg aactgggccg cgtcgagcag aacgggccgc
      961 gcggcggggg taagtctctt gaaggtcatc ggacgcagac atctaccggc caattctcac
     1021 cgaaccctta tgaaacaagg gttctggccc aatttgaggg gcgattcccg tgcggcctca
     1081 ggtcgcggcc agcttcgcca gaccggccgc gcgccacttc tgcgccagcg accatgcgat
     1141 cacgccgagc gcgaccacgg cgatcaggat cgtgcagacg gcgttgatct cgggcgtcac
     1201 gccgagccgc acctggctgt agatgcgcat cggcagggtc gtggcccccg ggccgctggt
     1261 gaagctggcg atgaccaggt cgtcgaggga cagggtgaag gccagcatcc agccggcgag
     1321 cacggcgggg aagatgttcg gcagggttac cttgagaaag gcctgggacg gcgtgcagcc
     1381 caggtcctgg gcggcttcct cgagcgaccg gtcgaaggtg atcagccgcg aatgcacgac
     1441 caccgcgacg taggacaggg tgaaggtggt gtgggccagc atcaccgtcc agaagccgcg
     1501 gtcgaaccgc agggcgacga atagcaacag caggctcagg cccatgatca cctcgggcat
     1561 gaccagcggc gcatagacca tgccggaaaa cagggtccgg ccgcggaacc gtccgccccg
     1621 caccagggcc accgccgcca gggagcccag cacggtcgcc gccgtggccg acaacagccc
     1681 gatgcgcagc gtcgtccagg ccgcgtcgag cagctgatgg tccttcatca gcgccacgta
     1741 ccagcggacc gaaaagccgc cccagacggt aaccagccgg ctggcgttga acgaccagac
     1801 caccagcagc acgatcggga gatagagaaa ggcgaacccg acggtcagcg acaccaggtt
     1861 gaaccaggac ggaccgcgcc tcatgacccg gcctccagcg ccttctgctg ctgccgctgg
     1921 tagagcacga tcggcgcgat cagggtgacc agcaggacga tcgccacggc cgaggcgaac
     1981 ggccagtcgc ggttggcgaa gaactccatc cagatcgagc ggccgatcat cagcgacccg
     2041 ggtccgccga gcagatcggg aatgacgaac tcgccgacca tcgggatgaa gcacagcagg
     2101 cagcccgcga tcaccccggg gatcgagagc ggccaggtga cctgccagaa gctcttccac
     2161 ggcggcgagc cgaggtcggc ggccgcctcc agcagggtgt cgtcaagccg ttccagcgcc
     2221 gcgtacagcg gcagcaccat gaacggcagg tagctgtaga cgaggccgat ctgcacggcc
     2281 gcgggcgtgt agagaatgtc cagcgggccg aggccgaaca gccccaggaa ggcgttgagc
     2341 agaccctggg gcttgaggat gccgacccag gcgtagaccc ggatcaggaa gctggtccag
     2401 aacggcagga tgacca
//
LOCUS       DAORNX010000446         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_32955_length_2415_cov_8.664407, whole genome shotgun sequence.
ACCESSION   DAORNX010000446 DAORNX010000000
VERSION     DAORNX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32955_length_2415_cov_8.664407"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLO65_15010"
     CDS             <1..560
                     /locus_tag="PLO65_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRD29608.1"
                     /translation="WKASSPAPQTVARASCIDTSGDRPCATPAAAMASISPALPLPGG
                     GATRFQPVFVGDVAAAVARAVSDPDCAGRTYELGGPGVFTFRELMAFVLSETGKRRLL
                     VPLPWPAASLIGRVAALTSVVGIAPVLTVDQVELLRSDNIVSEGAAGLAALGVTARSV
                     EAIAPTYLYRYRKGGQYAAAPEGAF"
     gene            complement(655..1458)
                     /locus_tag="PLO65_15015"
     CDS             complement(655..1458)
                     /locus_tag="PLO65_15015"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRD29609.1"
                     /translation="MTDLLAALILGVVEGLTEFIPVSSTGHLLLTKVALGLTDPSWDS
                     FIVLIQLGAILAVVALYFRRLWKVLIGLPTSPEARRFALSVLIAFLPAVIFGFLLYDF
                     IKQVLFESPGVICWSLIVGGVVLLAIDRWSPAPREHDAMKLPLLTSVGVGLFQCLAMI
                     PGVSRSGATIVGGVMLGVDKRAAAEFSFYLAIPTMVGAFAKDFWESRESLMVGDHLGV
                     LAVGFVVSFVSGLVVVKVLLDFVSKRGLAPFGWWRILVGAVGLAWIYLA"
     gene            1648..>2415
                     /locus_tag="PLO65_15020"
     CDS             1648..>2415
                     /locus_tag="PLO65_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRD29610.1"
                     /translation="MAERMLKFTTIERRTPEKRDARARDRDFHEIYADFIDAKAAEQA
                     SRCSQCGVPFCQTHCPLHNNIPDWLRLTAAGRLEEAYRLSQATNAMPEICGRICPQDR
                     LCEGNCVIEQSGHGTVTIGAVERYLTDKAWEEGWVKPLVVRADIGQSVGIVGAGPAGL
                     AAAEVLRELGYGVTVYDRHDRPGGLLIYGIPGFKLEKDVVERRTRRLADGGVAFRMNF
                     EVGRDATLPQLRERHDAVLLATGVYAARELEMPGAGSD"
BASE COUNT          421 a          843 c          815 g          336 t
ORIGIN      
        1 attggaaggc cagctccccc gcgccgcaga ccgtggcgcg ggcaagctgc atcgacacct
       61 cgggcgacag gccctgcgcc acgcccgccg cggccatggc ctcgatcagt cccgccctgc
      121 cgctgcccgg cggcggcgcc acgcgattcc agccggtctt tgtcggcgac gtggccgccg
      181 cggtggcccg ggccgtcagc gatcccgact gcgccggccg cacctacgaa ctcggcgggc
      241 cgggcgtgtt caccttccgc gagctcatgg ccttcgtgct gtccgagacc ggcaagcggc
      301 gccttctggt tccgctgccc tggccggcgg cgtccctgat cggcagggtc gcggcgctga
      361 cctcggtggt cggcatcgcc ccggtgctga ccgtcgacca ggtcgagctg ctgcgcagcg
      421 acaatatcgt gagcgagggg gcggccggcc ttgcggcgct cggcgtcacg gcgcgcagcg
      481 tcgaggccat cgccccgacc tacctttacc gctaccgcaa gggcggccag tacgcggccg
      541 cgcccgaggg ggcgttctga cgaaagcggg gacatgtact tcagtacgtg tccccccaat
      601 gccttcgaag ctgatggggg acacgtacca agaggtacat gtccccgcct gtggctaggc
      661 cagatatatc cacgccaggc cgaccgcccc gaccaggatc cgccaccagc cgaacggcgc
      721 cagaccgcgc ttgctgacga agtcgagcag caccttcacc accaccaggc ccgaaacgaa
      781 cgagaccacg aaaccgaccg ccaggacgcc caggtggtcg ccgaccatca ggctctcgcg
      841 gctctcccag aagtccttgg cgaaggctcc gaccatggtc gggatggcca ggtagaagct
      901 gaactcggcc gccgcccgct tgtcgacgcc cagcatcacg ccgccgacaa tggtcgcgcc
      961 cgagcgtgag acgcccggga tcatcgccag gcactggaac agcccgacgc cgacggaggt
     1021 caggagcggc agcttcatcg cgtcgtgctc tcgcggcgcg gggctccagc ggtcgatggc
     1081 cagcagcacc acgccgccga cgatcagcga ccagcagatc acgcccggac tctcgaacag
     1141 cacctgcttg atgaagtcgt acagcaggaa gccgaagatc accgcgggca ggaaggcgat
     1201 cagcaccgac agggcgaagc gccgggcctc ggggctggtg ggcaggccga tcaggacctt
     1261 ccacagccgc cggaagtaca gcgccaccac cgccaggatc gcgcccagct ggatcagcac
     1321 gatgaagctg tcccaggagg ggtccgtcag gcccagcgcg accttggtca acagcaggtg
     1381 gccggtggag gacaccggga tgaactccgt cagcccctcg acgacgccga ggatgagggc
     1441 ggccagaagg tcggtcatag gagcctcacg ggcatatggt ccactttatg gacgtaaatt
     1501 cgaggagcgc gccgcgacgg gcgcccagtt tcgcgacatt ggacgtgatt atcgtcaaca
     1561 ttattgacga tagcattttt cgtcccctgg ctgggctcta gaaggcccgt gaagaaaact
     1621 ttttctgcag gaaggctgga gcgcggcatg gccgagcgga tgctgaaatt caccaccatc
     1681 gagcgccgta ctccggagaa gcgggacgcg cgcgcgcgcg accgcgactt ccatgaaatc
     1741 tacgccgact tcatcgacgc caaggccgcc gaacaggctt cgcgctgttc gcagtgcggg
     1801 gtccccttct gccagaccca ctgcccgctg cacaacaata tccccgactg gttgcgactg
     1861 accgccgccg ggcggctgga ggaggcctat cgcctgtcgc aggcgaccaa cgccatgccg
     1921 gaaatctgcg gccggatctg cccgcaggac cggctgtgcg aaggcaactg cgtgatcgag
     1981 caatccggcc acggcacggt gaccatcggc gcggtcgagc gctacctgac cgacaaggcc
     2041 tgggaggagg gctgggtgaa gccgctcgtg gtccgggcgg acatcggcca gtcggtcggc
     2101 atcgtcggcg ccggcccggc cggcctggcc gccgccgagg tgctccgcga gctgggctat
     2161 ggggtcaccg tctatgaccg tcacgaccgc cccggcggcc tgctgatcta cggcatcccc
     2221 ggattcaagc tggagaagga cgtggtcgag cgccgcaccc ggcggctggc ggacggcggc
     2281 gtggccttcc ggatgaactt cgaggtcggc cgcgacgcca ccctgccgca actgcgcgaa
     2341 cggcacgacg cggtgctgct ggcgacaggc gtctacgcgg cccgcgaact ggagatgcca
     2401 ggcgcgggct cggac
//
LOCUS       DAORNX010000447         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33226_length_2400_cov_7.170149, whole genome shotgun sequence.
ACCESSION   DAORNX010000447 DAORNX010000000
VERSION     DAORNX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33226_length_2400_cov_7.170149"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLO65_15025"
     CDS             complement(<1..283)
                     /locus_tag="PLO65_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD29611.1"
                     /translation="MTTTIAEASLRDLGFSEIEARLYCELVRAGPATGYRLAKAVGKA
                     PTNTYQALEALLARGAVIVDDGEARTWRAAAPVELMAGLKAAFDRSGAAA"
     gene            complement(360..1013)
                     /locus_tag="PLO65_15030"
     CDS             complement(360..1013)
                     /locus_tag="PLO65_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29612.1"
                     /translation="MFVGHYAAAIAGKAIAPRAPLWSYIGAAQLLDIGWSSLVMAGVE
                     KVSFDPGLPGSPLVLEHMPWTHSLPAAVLWSVGGLLLARFLLRVPWWTAAMVGLVVFS
                     HWATDWLVHRPDLELWPGGEKVGLALWNFPGAEKTLEMGLIALAGACWLWNRGRFGRT
                     AWPALLFLTFLVALQMVGEFSEPAGDSFAFARLGLVVYLAVTAVAWLADLGRNRDEA"
     gene            complement(1059..>2400)
                     /locus_tag="PLO65_15035"
     CDS             complement(1059..>2400)
                     /locus_tag="PLO65_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HRD29613.1"
                     /translation="ARPPQPTAPVVPQPLDFAGEESAAKRARVGEGLARDGADASVIT
                     APASIAWLFNVRGGDVIRSPLPIGQAVLNADGTARLFLDPAKVTEDLPAWLGNQVTLE
                     PPEALAGALSDLKGRKVLVDPAQSSAWYFDTLAAEGATVVRGMDPCTLPRACKNAVEL
                     EGAKRAHIRDGAALTNFLHWIATEGQVNPPDEKEAVAKLEAFREATGALKDLSFDTIG
                     AANGHGALPHYRPTERSNERCAPGSLLLVDSGGQYLDGTTDVTRTMAIGVPSEEMRQR
                     FTLVLKGHLHLARVRFPVGTTGSALDVLARAPLWNAGLDYDHGTGHGVGVYLGVHEGP
                     ARIAKAPNSIALRPGMILSNEPGYYKPGEYGIRIENLQFVTEASDVPGGERPMLAFEN
                     LTWAPIDRTLIDVDLLTDEERAQMDQYHARVLDLIGPLVEAPVRAWLETACAPL"
BASE COUNT          364 a          854 c          835 g          347 t
ORIGIN      
        1 gggccgccgc gcctgagcgg tcgaatgcgg ccttcagccc ggccatcagt tcgaccggcg
       61 ccgccgcgcg ccaggtccgg gcctcgccgt cgtcgacgat caccgcgccc ctcgccagca
      121 gcgcctccag ggcctggtag gtgttggtcg gcgccttgcc caccgccttc gccaggcggt
      181 agccggtcgc cggcccggcg cggaccagtt cgcaatagag gcgcgcctcg atctcgctga
      241 acccgaggtc gcgcaggctg gcttcggcaa tagtagttgt catgactact actattttcg
      301 acaggcgccg tccggtcgag tgaaaccttg gtaatggcgc cgtggtcagc cggtgggcgc
      361 tacgcctcgt cccggttcct gccgagatcc gccagccagg ccacggccgt gaccgccagg
      421 taaacgacca gcccgagccg ggcgaaggca aagctgtccc cggccggttc ggagaattcc
      481 ccgaccatct gcagggcgac caggaaggtc agaaacagca gggcgggcca ggcggtgcgg
      541 ccgaaccggc cgcggttcca cagccagcag gcgccggcca gggcgatcag gcccatctca
      601 agcgtctttt ccgctcccgg aaagttccac agggccaggc cgaccttctc gccgccaggc
      661 cagagttcga ggtccggtcg gtggaccagc cagtccgtcg cccagtggga aaacaccacc
      721 agtccgacca tcgccgccgt ccaccacggc acgcgcagca gaaaacgcgc cagcaacagg
      781 ccgccgaccg accagagcac ggcggccggc aggctgtggg tccagggcat gtgctccagc
      841 accagcgggc tgcccggcaa cccgggatcg aagctgacct tctccacccc ggccatgacc
      901 aggctgctcc agccgatatc cagcagctgg gccgcgccga tgtagctcca cagcggcgcc
      961 cttggcgcta tggccttgcc cgcgatcgct gcggcgtaat gtccgacaaa catggcgtcc
     1021 tcccccgacg accggtcagg agaagagggt gaaccggttc agagcggcgc gcacgcggtt
     1081 tcgagccacg cgcgaaccgg ggcctcgacc aacggaccga tcaggtcgag cacccgggcg
     1141 tgatactggt ccatctgcgc ccgctcctcg tcggtcagca ggtcgacatc gatcagcgta
     1201 cggtcgatcg gcgcccaggt caggttctcg aacgccagca tcggccgctc gccgcccgga
     1261 acgtcgctcg cctcggtgac gaactgcagg ttctcgatgc ggatgccgta ctcgcccggc
     1321 ttgtagtagc ccggctcgtt gctgaggatc atgccgggcc gcagggcgat gctgttgggg
     1381 gccttggcga tgcgcgccgg tccctcgtgg acgcccagat agacccccac gccatggccc
     1441 gtgccatggt cgtagtcgag cccggcgttc cacagcggcg cccgggccag aacatcgagc
     1501 gccgatccgg tggtgccgac cggaaaacgc acccgcgcca gatggaggtg gcccttgagc
     1561 accagggtga agcgctggcg catctcctcg ctcgggacgc cgatggccat ggtccgggtg
     1621 acgtcggtgg tgccgtccag gtactggccg ccggagtcca ccagcagcag cgagccgggc
     1681 gcgcaacgct cgttgctgcg ctcggtgggg cggtagtggg gcagggcgcc atgaccgttg
     1741 gccgcgccga tggtgtcgaa gctcagatcc ttgagcgcgc cggtggcctc gcggaaggcc
     1801 tccagcttcg ccacggcttc cttctcgtcc ggcggattga cctgtccttc ggtggcgatc
     1861 cagtggagga agttggtcag ggccgccccg tcgcggatat gggcccgctt ggcgccctca
     1921 agctcgacgg cgttcttgca ggctcggggc agggtgcagg ggtccatgcc gcgcaccacg
     1981 gtcgcgccct cggcggccag ggtgtcgaaa taccaggccg aggactgggc cgggtcgacc
     2041 agcaccttcc ggcccttgag gtccgacagc gccccggcca gcgcctcggg cggctccagc
     2101 gtcacctggt tgccgagcca ggcgggcaga tcctcggtca ccttggccgg atcgaggaac
     2161 agccgcgccg ttccgtcggc gttgagcacc gcctggccga tgggcagggg cgatcggatg
     2221 acatcgccgc cgcggacatt gaacagccag gcgatcgagg ccggcgcggt gatcaccgag
     2281 gcgtcggcgc cgtcgcgcgc cagaccttcg ccgacgcggg cccgcttggc ggccgactcc
     2341 tcgccggcga agtccagcgg ctgcgggacg accggcgccg tcggctgcgg cggccgggcg
//
LOCUS       DAORNX010000448         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33363_length_2392_cov_7.184852, whole genome shotgun sequence.
ACCESSION   DAORNX010000448 DAORNX010000000
VERSION     DAORNX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33363_length_2392_cov_7.184852"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLO65_15040"
     CDS             complement(<1..749)
                     /locus_tag="PLO65_15040"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRD29614.1"
                     /translation="MPYRFVPALLALLFAIPAFADPAWTGTWNVSARFAGGEASSAMT
                     LTAGDGRVMGVSGPLDENRFFPLTITGVPVDDAGARLEMRSAGALVGTLAITVHRGAL
                     AGTGELYGTPVTLVGVRPDTAPAGPGATYDFRPTTYALQYSARGEPALRIRPGDRVST
                     STLDNKGRDSALVWRAMPGNSLTGPFYVEGAMPGDTLAVHIERVALNRDTAEMYSGAL
                     DRRAVPGGYVQSPTPGWGRTWTLDRARGVARP"
     gene            908..>2392
                     /locus_tag="PLO65_15045"
     CDS             908..>2392
                     /locus_tag="PLO65_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRD29615.1"
                     /translation="MDRPGTIEASQSDDGRYRLLVEAITDYAIYMLDPEGRVTSWNAG
                     AQRFKGYQPAEIIGQHFSRFYTDEDRAAGVPAEALRRAREESRFETEGWRVRKDGGRF
                     WAHVLIDPIRSPQGELIGFAKITRDLTERRAAQASLIRSQEQFRLLVQGVTDYALYML
                     DLDGRVTNWNAGAERIKGYRPEEIIGQHFSRFYTPGDREAGEPARALRTAAAEGRFEK
                     EGWRVRKDGTRFWAHVVIDPIRDDAGEPIGFAKITRDITERRDAQAALEEARQALFQS
                     QKLEAIGQLTGGIAHDFNNLLMAVLSSLELLRKRLPDDPRAVALLDNAVQGAQRGASL
                     TQRMLAFARKQDLRLEGVCVSDLVQGMAGLLERSAGPSIELTLDAPPGLGAAHTDPNQ
                     LETALLNLVVNARDAMPGGGRIRVEVNAARPEPDGQGDLPPGDYVRLVVADDGPGMDR
                     VTLARATEPFFTTKGVGKGTGLGLSMVHGLAEQSGGRLVLRSAPG"
BASE COUNT          356 a          851 c          879 g          306 t
ORIGIN      
        1 ggccgggcga cgccgcgggc gcggtccagc gtccaggtcc ggccccaccc gggagtcggc
       61 gactggacgt agccgccagg gaccgcgcgc cggtccagcg cgccgctgta catctccgcc
      121 gtgtcgcgat tgagcgccac ccgctcgatg tgcaccgcca gggtgtcccc gggcatggcg
      181 ccctcgacat agaagggacc ggtcaggctg ttgccgggca tggcccgcca gaccagcgcg
      241 ctgtcgcggc ccttgttgtc gagtgtcgag gtcgagaccc ggtcgccggg gcggatgcgc
      301 agggccggtt cgccccgcgc cgagtactgc agcgcatagg tcgtgggcct gaagtcatag
      361 gtggcgcccg ggccggcagg ggcggtgtcc ggccggacgc cgacgagtgt cacgggagtc
      421 ccgtagagct cgccggttcc ggccagcgcc ccgcgatgca cggtgatcgc cagagtcccg
      481 accaacgcgc cggcggaccg catctcgagc cgcgcccccg cgtcgtcgac ggggacgccg
      541 gtgatcgtca gcggaaagaa ccggttctcg tcgagcggcc ccgagacgcc catcacccgc
      601 ccgtctccgg cggtcagggt catcgcgctg gaggcctccc cgcccgcgaa tcgcgcgctg
      661 acgttccagg ttccggtcca cgccggatcg gcgaacgccg ggatggcgaa gagcagcgcg
      721 agcagggctg gaacgaatcg atacggcatg acccttcccc tggcggcgga cgcccatcct
      781 agcgccttca tgcgccagcg cgagccggcc gcgcggatgg acgccaccct tcgggcctgc
      841 aagggaactc cttcgcccgg tcgggcgtta gcggccggtg gcagggctgt gcgcccagcg
      901 gggtgtaatg gaccgtccgg gtacgatcga ggcctcccag agcgatgacg gccgctaccg
      961 gttgctggtc gaggcgatca ccgactacgc catctacatg ctcgaccccg aagggcgggt
     1021 gaccagctgg aacgccgggg cccagcggtt caagggctac cagcccgccg agatcatcgg
     1081 ccagcatttc agccgcttct acaccgacga ggatcgcgcc gcgggggttc cggccgaggc
     1141 gttgcgccgg gcccgcgagg aaagccgctt cgagaccgag ggctggcgcg tgcgcaagga
     1201 cggcgggcgg ttctgggccc atgtgctgat cgatccgatc cggtcgccgc agggcgagct
     1261 gatcggcttc gccaagatca cccgcgatct gaccgagcgg cgggccgccc aggcgagcct
     1321 gatccgcagc caggaacagt tccgcctgct ggtccagggc gtgaccgact acgccctgta
     1381 catgctcgac ctcgacggcc gggtgaccaa ctggaacgcc ggggccgagc ggatcaaggg
     1441 ctaccggccc gaggagatca tcggccagca cttcagccga ttctacacgc ccggggaccg
     1501 cgaggccggt gagccggcgc gggccctgcg gacggcggcg gccgaggggc ggttcgagaa
     1561 ggagggctgg cgcgtccgca aggacgggac ccggttctgg gcccatgtgg tgatcgaccc
     1621 gatccgcgac gacgccggcg agccgatcgg cttcgccaag atcacccgcg acatcaccga
     1681 acgccgcgac gcccaggccg cgctcgagga agcccgccag gcgctgttcc agtcgcagaa
     1741 gctcgaagcc atcggccagc tgaccggcgg catcgcccat gacttcaaca acctgctgat
     1801 ggccgtgctg agcagtctgg agctgttgcg caagcgcctg cccgacgacc cgcgcgccgt
     1861 ggcgctgctg gacaacgccg tgcagggggc ccagcgcggc gccagcctga cccagcgcat
     1921 gttggccttc gcccgcaagc aggacctgcg gctcgagggc gtctgcgtgt ccgacctggt
     1981 gcagggcatg gcggggctgc tcgaacggtc ggcggggccg tcgatcgaac tgaccctgga
     2041 cgcgccgccc gggcttggag ccgcccatac cgaccccaac cagctggaga cggcgctgct
     2101 gaacctggtg gtcaacgccc gggacgccat gcccgggggc ggacggatcc gggtcgaggt
     2161 caacgccgcc cggcccgagc ccgacgggca gggagacctg ccgcccggcg actacgtccg
     2221 gctggtcgtc gccgacgacg gcccgggcat ggaccgggtg accctggcgc gcgccaccga
     2281 gccgttcttc accaccaagg gcgtgggcaa gggcaccggc ctgggcctgt cgatggtcca
     2341 cggcctggcc gagcagtcgg gcggccggtt ggtgctgcgc agcgcgccgg ga
//
LOCUS       DAORNX010000449         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33424_length_2388_cov_7.050150, whole genome shotgun sequence.
ACCESSION   DAORNX010000449 DAORNX010000000
VERSION     DAORNX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33424_length_2388_cov_7.050150"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="pgi"
                     /locus_tag="PLO65_15050"
     CDS             <1..1070
                     /gene="pgi"
                     /locus_tag="PLO65_15050"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRD29616.1"
                     /translation="LEPQVELRFVGNVDPADIAQALAGLDAETTLVVVVSKTFTTQET
                     MANATAARAWLRAFLGEAGDSHLAAASTNLEACAAFGIPAERVFGFEDWVGGRYSVMS
                     AVGLSCAIALGWEAFAGLQAGAAAMDDHFRTAPLAANAPVLLALAHVFNRNGLGRPVR
                     VVEPYADRLGLLASYLQQLEMESNGKRVTLSGAPVAHATATAVFGNAGTNDQHAFFQM
                     LHQGTDVVPVDFVAVARAAEGPAPQQAILLANAIAQAEALMTGSANAAEPHRDFPGDR
                     PSSFILLDRLTPAALGALIALYEHKVFVEGVLWDINSFDQWGVELGKTLASAVLGELR
                     GGDAGTHDPSTAALIERLRSP"
     gene            1388..>2388
                     /locus_tag="PLO65_15055"
     CDS             1388..>2388
                     /locus_tag="PLO65_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HRD29617.1"
                     /translation="MTDQTFRPEARAPRGFADKRAATLRAERRIVEAVSKVYEQWGFE
                     PLDTGAFEYADALGKFLPDSDRPNEGVFALQDDDEQWMALRYDLTAPLARFAAQNWET
                     LPKPYRRYAFGPVWRNEKPGPGRFREFIQCDADTVGSARPEADAEIIALAVAGLEAAG
                     LPRGAAVMKINNRKLLNGLLTAAGVTTEGQKLGVLRAVDKLDRLGREGVALLLGEGRK
                     DESGAFTQGAGLGARAIESVLAFVAAGQPDRSGTLDALARVVGGSAEGDEGLEELARI
                     SGALKGLGVGDDQASFDPSIVRGLEYYTGAVFEAELLLETTDDRGQPVRFGSIGGGGR
                     "
BASE COUNT          344 a          870 c          844 g          330 t
ORIGIN      
        1 ccctggagcc gcaggtcgag ctgcgcttcg tcggcaacgt cgacccggcc gacatcgccc
       61 aggcgctggc cgggctggac gcggagacca ccctggtcgt ggtcgtctcc aagaccttca
      121 ccacccagga gaccatggcc aacgccacgg ccgcccgggc ctggctgcgg gccttcctcg
      181 gcgaggcggg ggacagccat ctggcggcgg cctcgaccaa cctcgaggcc tgcgcggcct
      241 tcggcattcc ggccgagcgc gtgttcggct tcgaggactg ggtcggcggc cgctattcgg
      301 tgatgtcggc ggtcgggctt tcgtgcgcca tcgcgctggg ctgggaggcc ttcgccgggc
      361 tgcaggcggg cgccgccgcg atggacgacc acttccgcac cgcgccgctg gcggccaatg
      421 cccccgtgct gctggccctg gcccatgtgt tcaaccgcaa cggccttggc cgacccgtgc
      481 gggtggtcga gccctatgcc gaccgcctgg gcctgctggc cagctacctg cagcagctgg
      541 agatggagtc caacggcaag cgggtgaccc tgtccggcgc gccggtcgcc cacgccacgg
      601 ccacggccgt gttcggcaac gccggcacca acgaccagca cgccttcttc cagatgctgc
      661 accaggggac cgacgtcgtc ccggtggact tcgtcgccgt ggcccgggcc gccgaaggtc
      721 ccgccccgca acaggcgatc ctgctggcca acgccatcgc ccaggccgag gccctgatga
      781 ccggctcggc caacgccgcc gagccgcacc gcgacttccc cggtgaccgg ccctccagct
      841 tcatcctgct cgaccgcctg acgcccgccg cgctcggcgc cctgatcgcc ctctatgagc
      901 acaaggtgtt cgtcgagggc gtgctttggg acatcaacag cttcgaccag tggggcgtgg
      961 agctgggcaa gaccctggcg tcggccgtgc tcggcgagct gcggggcggc gacgcgggga
     1021 cgcacgatcc gtcgacggcg gcgctgatcg agcggttgcg cagcccataa atattgtcat
     1081 cccggccgca gggcggagcc cggagagccg ggacccagat tcggcttcat ccgggacgag
     1141 attcaatccg ggtcccggat agccgctgcg cggcttccgg gatgacagtg gagatttgct
     1201 tcgctatatc cccgctccga tgacccgcgc cccgcaccat catcacgcgc aacaccacgc
     1261 cttccctcgc gggacggaac gggttcgcgc ggcctagagc caaggcgcac tccctccgac
     1321 cccgcccgac gcggatgggg tctgatggct ccatccggcc ccgcaagacc gaatgtagag
     1381 ccgaccgatg accgatcaga cctttcgccc cgaagcccgc gcgccgcgcg gattcgccga
     1441 caagcgcgcc gcgaccctgc gcgccgagcg gcgcatcgtg gaggcggttt cgaaggtcta
     1501 cgagcagtgg ggcttcgaac cgctcgacac cggcgccttc gagtacgccg acgccctggg
     1561 caagttcctg cccgacagcg accggcccaa cgagggcgtg ttcgcgctgc aggacgacga
     1621 tgagcagtgg atggccctgc gctacgacct gaccgcgccg ctggcccggt tcgcggcgca
     1681 gaactgggaa accctgccca agccctatcg ccgctacgcc ttcgggccgg tctggcgcaa
     1741 cgagaagccg ggcccgggcc ggttccgcga gttcatccag tgcgacgccg acacggtggg
     1801 ctcggcgcgg cccgaggcgg atgcggagat catcgccctg gccgtcgccg gccttgaagc
     1861 cgccggcctg ccgcgcgggg cggcggtcat gaagatcaac aaccgcaagc tgctcaacgg
     1921 cctgctgacg gcggctggcg tgaccaccga gggccagaag ctgggcgtac tgcgggccgt
     1981 ggacaaactg gaccggctgg gtcgcgaggg cgtggccctg ttgctcggcg aaggccgcaa
     2041 ggacgagtcc ggcgccttca cccagggcgc gggcctcggc gccagggcca tcgaatcggt
     2101 gctggccttc gtcgccgccg gccagcccga ccggtccggc acgctggacg cgctggccag
     2161 ggtcgtcggc ggctcggccg agggcgacga ggggctggag gagctggcca ggatcagcgg
     2221 cgcgctcaag ggcctcggcg tcggcgacga ccaggccagc ttcgacccct ccatcgtgcg
     2281 cggcctggaa tactacaccg gcgcggtgtt cgaggcggag ctgctgctgg agaccaccga
     2341 cgacaggggc cagccggtgc gcttcggctc gatcggcggc ggcgggcg
//
LOCUS       DAORNX010000450         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33534_length_2382_cov_6.835840, whole genome shotgun sequence.
ACCESSION   DAORNX010000450 DAORNX010000000
VERSION     DAORNX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33534_length_2382_cov_6.835840"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1276)
                     /locus_tag="PLO65_15060"
     CDS             complement(152..1276)
                     /locus_tag="PLO65_15060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29618.1"
                     /translation="MNFDFSDEQKQLQDQARRYLADKCPPAAVRAVLEGGQSYDRDLW
                     VGLGELGFLGAAIPEEYGGVGLGHLELCVIAEELGRALAPVPVASSIYLAAEFLMAAG
                     SEDQKRAWLPKLASGETIGCFAYGEGLGRVTETSIKATVSGGRLSGTKTPVADGDIAD
                     LAVVAAREGGKVSLYLVDLTGPGVTRDVVESIDPTRSQSALTFRDAPAERLGAAGEGW
                     RLAAEVMDRAAILLAFEQVGGADRALEMGRDYALERMAFGRQIGSFQAIKHMLADMYV
                     SATLARSNAYYGAWALSTGSAELPVAAAMARVSATQAFQHCSKNNIQVHGGMGFTWAF
                     DCHLFYRRSNNLALALGSQSTWEDLLIDRLRQRNDAPAAA"
     gene            1418..2038
                     /locus_tag="PLO65_15065"
     CDS             1418..2038
                     /locus_tag="PLO65_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29619.1"
                     /translation="MITVFGGQTSRSIKVVWLLEEIGLPYRVRQVDMFRAAEDLEFLA
                     ANPAGYIPVLRDGDVTMVESIAIMEYLMGRYGPTPLAPAPTDPAFPVYQQFLHLGEAG
                     IATLLMVPVVSGFMAPEAERDNWGARLAKTWVGNRLKLVSAQLARTPWIAGEAFTAAD
                     ISITYALQFARKQGGMTLGEAEEAYLARATARDAYKRAFETSHAGA"
BASE COUNT          356 a          838 c          810 g          378 t
ORIGIN      
        1 cgacgctttt gaggccgttg ttggcgaagc cgccctgctt cagttcggcc tccagttccc
       61 tgggcgcgtt ggcctcgatc caggcgcggg cggtggcgcg gaaggcggcc tcttcggggg
      121 tgtcgtcgaa gttcatctgt ccgttccttc ttcaggcggc ggccggagcg tcgttgcgct
      181 ggcgcaggcg gtcgatcagc aggtcttccc aggtcgactg ggagcccagc gccagggcca
      241 ggttgttgga ccgccgatag aacagatggc agtcgaaggc ccaggtgaag cccatgccgc
      301 cgtgcacctg gatgttgttc ttgctgcagt gctggaaggc ctgggtcgcc gagacccgcg
      361 ccatggccgc cgccaccggc agctccgccg agccggtcga aagggcccag gcgccgtagt
      421 aggcgttcga ccgggccagg gtcgccgaca catacatgtc ggccagcatg tgcttgatcg
      481 cctggaaaga gccgatctgg cgaccgaagg ccatccgctc gagggcgtag tcgcgaccca
      541 tctccagcgc ccggtcggcg ccgccgacct gctcgaaggc cagcaggatg gccgcccggt
      601 ccatgacctc cgccgccagc cgccagcctt cgccggccgc gcccagccgc tcggccgggg
      661 cgtccctgaa cgtcagcgcc gactggctgc gggtcggatc gatgctctcg acgacgtccc
      721 gggtcacgcc cgggccggtg aggtcgacca ggtacagcga caccttgccg ccctcgcgcg
      781 ccgcgacgac ggccaggtcg gcgatgtcgc cgtcggcgac cggggtcttg gtcccgctca
      841 gtctgccgcc cgagacggtc gccttgatcg acgtctcggt cacccggccc aggccctcgc
      901 cataggcgaa acagccgatg gtctcgcccg acgccagctt cggcagccag gcccgcttct
      961 ggtcttcact gcccgccgcc atcaggaatt cggcggccag atagatcgac gaggcgaccg
     1021 gcacgggcgc gagcgcgcgg cccagctcct cggcgatcac gcacagctcc agatggccca
     1081 ggccgacgcc gccgtattct tccgggatcg cggccccgag aaagccgagc tcgccgaggc
     1141 cgacccacag atcgcgatcg tagctctgcc cgccctccag cacggcccgc acggcggcgg
     1201 gcggacattt gtcggcgaga taccgccgcg cctggtcctg cagctgcttc tgctcgtcgg
     1261 agaaatcgaa gttcatgcgg gcctcccgga acgtctcttg cgcccacttg aacgccctca
     1321 cgcgggggca gccaagccct ctcacgcaca cgcagggggt gattgtgccg ccccggcatg
     1381 gcacgatccg ccgcagtcac aggcaggaag ccagtccatg atcaccgtct tcggcggaca
     1441 gacctcgcgg tccatcaagg tcgtctggtt gcttgaggaa atcggcctgc cgtatcgggt
     1501 gcggcaggtg gacatgttcc gcgcggccga agacctcgag ttcctggccg ccaaccccgc
     1561 aggctacatt ccggtgcttc gggacggcga cgtgaccatg gtcgagtcga tcgcgatcat
     1621 ggaatacctg atgggccgct acggtccgac cccgctcgcg ccggcgccga ccgatcccgc
     1681 ctttccggtc taccagcagt tcctgcatct gggcgaagcg ggtatcgcga ccctgctgat
     1741 ggtcccggtc gtcagcggct tcatggcgcc ggaggccgag cgcgacaact ggggcgcccg
     1801 tctggcgaag acctgggtgg gcaaccgcct gaagctcgtt tcggcgcagc tggcccgcac
     1861 gccctggatc gccggcgagg ctttcacggc ggccgacatc tcgatcacct acgccctgca
     1921 gttcgcccgc aagcagggcg gcatgacgct cggtgaggcg gaggaggcct acctcgcccg
     1981 cgccacggcc cgcgacgcct acaagagggc cttcgagacg tctcacgccg gggcgtagcg
     2041 tagggggcat ctggcggccc tccctaaagc ctcgtcatgg cccgacttgt tcgggccacc
     2101 caagcgtggg ctgttggacg atgactgcgc agcgacccat cgcgctcatc ctgcatcgat
     2161 ggtgttcctg ggtggcccga acaagtcggg ccatgacggc gtgcggggtg ttgcctgacg
     2221 ctgggtcgcc agcgtcagat ggccgccaac gtacaagaac tcacgggaga gcgcaccatg
     2281 gatctggggc tgaaggacag gaccatcttc gtcgccggcg ccagccgcgg catcggcctg
     2341 ggcatcgttg aggcctgcct ggccgagggc gcgagggtgg cc
//
LOCUS       DAORNX010000451         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33557_length_2381_cov_8.133706, whole genome shotgun sequence.
ACCESSION   DAORNX010000451 DAORNX010000000
VERSION     DAORNX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33557_length_2381_cov_8.133706"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLO65_15070"
     CDS             <1..275
                     /locus_tag="PLO65_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29620.1"
                     /translation="DGGDRFGLVLMDLRMPDLSGVEVSRQLRARGVTTPIAALTADAF
                     EDDRRAALAAGMNDFLVKPLTEAALRAVLARWVGWTGEAGEGRLAS"
     gene            323..2038
                     /locus_tag="PLO65_15075"
     CDS             323..2038
                     /locus_tag="PLO65_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRD29621.1"
                     /translation="MPSENQAVEPVARPTFLQSLATFGERRTLVMLVLGFSAGLPNLL
                     VFDTLQIWLREAGLSLAAISVFGLATIATGMKFLWAPLVDRTRVPMLTALFGHRRSWM
                     LVSQAVIMLGLWLIAGVNPATNLGVMAAFAVMVSFASANQDIVIDAWRIEAADDARQG
                     PMAAAYQWGYRIAMIVAGAAPLILAELYSWRISYAAMAALMILGVAAVFAAPREAEHS
                     VRPIPMGEMTPRPALEVAEWVLRLAILVAGGLLLGSGLAASADFLASILATLGAGGLG
                     EAMKAAWASDNKVWFQLLAVVAGLGVIAAATLRIPGVSTRPGAYLSGALGQPLADFFG
                     RYGKVAGLILALICLYRISDFVLNINGAFYVDLGFSKLEIAEIRKVFGVVMSVIGVAL
                     GGMSVIRLGLMRAMVLGAFAGALSNLAFAWLATQGHSVNALLVAIAVDNVAGGVAGTC
                     LIAYMSSLTTAGFTATQYALFSSLYALPGKLLASQSGRIVESSARAADAGGPIAALNG
                     LFGRLPPESYATALEKSGVSSAALGAGYMVFYLYSTLIGVFAIVLAFMVARRTAEDSP
                     AVEPA"
     gene            complement(2035..>2381)
                     /locus_tag="PLO65_15080"
     CDS             complement(2035..>2381)
                     /locus_tag="PLO65_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRD29622.1"
                     /translation="NLVVRGRGARTEPVLAWAEIGRTDTLKGTLAGLSGAPVLDSAGR
                     VIGVTVAEAPRRGRIYTTTPETVRKALAGSQRRPSTYAVGEPINVENYGRVADGLRRD
                     LRVAQVVCLSTS"
BASE COUNT          297 a          851 c          829 g          404 t
ORIGIN      
        1 ccgatggcgg cgaccgcttc ggcctggtgt tgatggacct gcgcatgccc gacctgtccg
       61 gggtcgaggt gtcgcgccag ctgcgcgccc gcggggtcac cacgccgatc gccgccctga
      121 ccgccgacgc cttcgaggac gaccgccgcg cggccctggc cgccggcatg aacgacttcc
      181 tcgtcaagcc cctgaccgaa gccgccctgc gcgcggtcct cgcccgctgg gtcggctgga
      241 cgggcgaagc cggagagggc aggctcgcct cctgatttgg ggggcggagc gacccgcccc
      301 cgcttcgggg gaagcactgc ccatgcccag cgaaaatcaa gccgtcgaac cggtcgcccg
      361 gcccaccttt ctgcagtcgc tcgcgacctt cggcgagcgc cgcaccctgg tcatgctggt
      421 gctcggcttt tcggcgggcc tgcccaacct gctggtcttc gacacgctgc agatctggct
      481 gcgcgaagcg ggcctgtcgc tggccgccat cagcgtcttc ggcctcgcca ccatcgccac
      541 gggcatgaag tttctctggg cgccgctggt agaccggacc cgggtgccga tgctgaccgc
      601 cctgttcggc caccgccggt catggatgct ggtctcgcag gcggtgatca tgctcggcct
      661 gtggctgatc gcgggcgtca atccggccac caacctcggc gtcatggccg ccttcgcggt
      721 gatggtctcg ttcgcatcgg ccaaccagga catcgtcatc gacgcctggc ggatcgaggc
      781 ggctgacgac gcccgacagg ggccgatggc ggccgcctac cagtggggct atcgcatcgc
      841 catgatcgtg gcgggcgccg cgccgctgat cctggccgag ctctacagct ggcgcatttc
      901 ctacgcggcc atggccgcgc tgatgatcct cggcgtggcc gcggtcttcg ccgccccgcg
      961 cgaagcggaa cattccgtgc ggccgatccc gatgggcgag atgacgcctc gtcccgccct
     1021 cgaagtcgcg gagtgggtac tgcgcctcgc catcctcgtc gcgggcggcc tgctgctggg
     1081 ctcgggcctg gcggccagcg cagacttcct ggcgtcgatc ctggcgacgc tgggcgccgg
     1141 aggtctgggc gaggcgatga aggccgcgtg ggcgtcggac aacaaggtct ggtttcagct
     1201 gctcgccgtc gtcgcgggcc tgggcgtgat cgccgccgcg acgctcagaa ttcccggcgt
     1261 cagcacccgg cccggagcct atctttccgg cgcgctcggg cagccgctgg ccgatttctt
     1321 tggccgttac ggcaaggtcg ccggcctgat cctggccctc atctgcctgt atcgcatctc
     1381 tgacttcgtg ctgaacatca acggggcctt ctatgtcgac ctcggcttct ccaagctgga
     1441 gatcgccgag atcaggaagg tgttcggcgt cgtcatgtcg gtcatcggcg tcgccctggg
     1501 ggggatgtcg gtgatccgcc tgggattgat gcgggccatg gtgctgggcg ccttcgccgg
     1561 ggccttgagc aacctggcct tcgcctggct ggccacccag ggtcacagcg tcaacgcgct
     1621 gctggtcgcc atcgcggtgg acaacgtcgc cggcggcgtc gccggcacct gcctgatcgc
     1681 ctacatgtcg agcctgacca ccgccgggtt cacagcgacg cagtacgcgc tgttctcgtc
     1741 gctctacgcc ctgcccggga aactgctggc cagccagtcg gggcggattg tcgagagttc
     1801 ggcccgggcc gccgacgccg ggggtccgat cgccgccctc aacgggctgt tcggccgcct
     1861 gccgccggaa agctacgcca cggcgctgga gaagtccggc gtcagctccg ccgcgcttgg
     1921 cgccggctac atggttttct acctgtattc gacgctgatc ggcgtgttcg ccatcgtgct
     1981 ggccttcatg gtcgcgcgac ggacggcgga ggacagtccc gccgtcgagc cggcctagct
     2041 cgtcgacagg cagaccacct gggcgacgcg caggtcgcgg cgcaggccgt cggcgacgcg
     2101 gccgtagttc tcgacattga tcggctcgcc gacggcgtag gtcgacggac ggcgctgcga
     2161 gccggccagg gccttgcgca cggtctccgg cgtggtggtg tagatgcgtc cgcgccgggg
     2221 cgcctcggcg acggtgacgc cgatcacccg gccggccgaa tccagcaccg gcgcgcccga
     2281 caggcccgcc aaggtgccct tgagcgtatc ggtgcggcca atctcggccc aggccagcac
     2341 cggctcggtg cgggcgccgc ggccccggac caccaggttc t
//
LOCUS       DAORNX010000452         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33683_length_2373_cov_7.686368, whole genome shotgun sequence.
ACCESSION   DAORNX010000452 DAORNX010000000
VERSION     DAORNX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33683_length_2373_cov_7.686368"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLO65_15085"
     CDS             <1..393
                     /locus_tag="PLO65_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD29623.1"
                     /translation="DSLRIIGKGSKTRIAPVLPAVREAVDAYLAALPFTLDPDEPLFR
                     AARGGPLSPRHVQAIMQLLRGRLGLSDRATPHALRHSFATHLLGAGADLRSIQELLGH
                     ASLSTTQKYTAVDAAGLLAAYGKAHPRA"
     gene            complement(476..1390)
                     /locus_tag="PLO65_15090"
     CDS             complement(476..1390)
                     /locus_tag="PLO65_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29624.1"
                     /translation="MAKDGEPVWKDPDIGMVRQLLAMARPVGGPAQSWDERRMGMDAF
                     GLRPLADGTVRVGIDIDGLHSERLTPPGADETRALLYLHGGGYCIGSPLSHGVMVSRF
                     AAAMGVPAIVPNYRLAPEHPFPAAVDDALKAYRHLLEGVAPGRIAIAGDSAGGGLTLA
                     LALAIRDAGLPQPGCLFAISPWADLRQVGAAYAAIEPVDPMLSKAGLDEFATAYLAGH
                     DPADPLASPALGDYTGLAPLLIHVGGAEVLLSDACAVAEKAGLAGVDVRLEIWPEMIH
                     VWHAFADQMATSWRAIDEAAAWMKGKLT"
     gene            1542..1775
                     /locus_tag="PLO65_15095"
     CDS             1542..1775
                     /locus_tag="PLO65_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29625.1"
                     /translation="MKKIALAAALVSLLGVAACESKADKAAEAKADALEAQAAATPNE
                     AQETQLNKEADQVEQQAGNADGGATTENTPTTH"
     gene            complement(2075..>2373)
                     /locus_tag="PLO65_15100"
     CDS             complement(2075..>2373)
                     /locus_tag="PLO65_15100"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619213.1"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRD29626.1"
                     /translation="AAGVAYKVGKFPFTANSRAKINHEGEGFVKVLADAKTDRILGVH
                     MIGPNVGDMIAEYCVAMEFSASSEDVARTCHPHPTRSEALRQAAMGVEGWTMQA"
BASE COUNT          372 a          842 c          824 g          335 t
ORIGIN      
        1 gacagcctgc ggatcatcgg caagggctcc aagacccgaa tcgcgccggt gctgccggcc
       61 gtgcgcgagg ccgtggacgc ctatctggcc gccctgccct tcacgctgga tccggacgag
      121 ccgctgttcc gcgccgcccg tggaggtccg ctgagccccc gccacgtgca ggcgatcatg
      181 cagctgctgc gcggccgcct cggcctgtcg gatcgcgcca cgccccacgc cctgcgccac
      241 agcttcgcca cccacctgct gggcgccggc gccgacctgc gctcgatcca ggaactgctg
      301 ggccacgcct cgctgtcgac gacgcagaag tacacggcgg tcgacgcggc aggtctgctg
      361 gcggcctacg gcaaggccca tcccagggcg tagcaggggc gcggtgaccg gcgatcaata
      421 gccccgcgtc ggcgcgagcc atggcgcgga cagcctacgc cctacgccct acgccctaag
      481 tcagcttccc cttcatccac gccgctgcct cgtcgatcgc tctccagctg gtggccatct
      541 ggtcggcgaa ggcgtgccag acgtggatca tctccggcca gatctccagc cggacgtcca
      601 cgccggccag gcccgccttc tcggccacag cgcaggcgtc ggagagcagg acttcggcgc
      661 cgccgacatg gatcagcagc ggggcgaggc ccgtgtagtc gccgagcgcc ggcgaggcga
      721 gcggatcggc cgggtcatgg ccggccagat aggcggtcgc gaactcgtcg aggcccgcct
      781 tgctgagcat cgggtcgaca ggctcgatgg ccgcataggc cgcgccgacc tgacgcagat
      841 cggcccacgg actgatggcg aacagacagc ccggctgggg caggcccgcg tcgcgaatgg
      901 ccagggccag ggccagggtc aggccgccgc cggccgagtc gcccgcgatg gcgatgcgtc
      961 ccggcgcgac cccctcgagc aggtgtcgat aggccttcag ggcgtcgtca acggcggcgg
     1021 gaaagggatg ctccggggcc aggcggtagt tcggcacgat cgccgggacg cccatcgccg
     1081 cggcgaagcg ggagaccatc accccatggc tgagcgggct tccgatgcag tagccgccgc
     1141 cgtgcagata gagcagcgcc cttgtctcat cggcgccggg cggggtcagc cgctcgctgt
     1201 gcaggccatc gatgtcaatg ccgacgcgga ccgtgccgtc ggcgaggggc cgcaggccga
     1261 aggcatccat gcccatgcgg cgctcgtccc agctctgcgc cggtccgccg acgggcctgg
     1321 ccatcgccag cagctggcgg accatgccga tatcggggtc cttccagacc ggttcgccgt
     1381 ccttggccat gcgccgcctc cccttttccg gcgaggaggc taaccgggat cagccgggag
     1441 gcaaggggat cagacctgcc gccggcggga gtcgacaatt gttttgaacc cgggcgcggg
     1501 catcggcgtt gtccagccac gacgcaacaa aggctgttcc catgaaaaag atcgccctcg
     1561 ccgccgctct cgtgtccctg ctcggggtcg cggcctgtga aagcaaggct gacaaggccg
     1621 ccgaggccaa ggccgacgct ctcgaagccc aggccgcggc cacgccgaac gaggcgcagg
     1681 aaacgcagct gaacaaggaa gccgaccagg tcgagcaaca ggccggcaat gccgacggcg
     1741 gcgcgacgac cgaaaacacc ccgacgaccc actaggctcg tcacccccct gtgcggaacc
     1801 cccgtcgcgc ccgcggcggg ggttttgcct gccggacgtt gcgcgaggga cagggacttt
     1861 ggacaggcag gcagcctgtc accgcctggc cagggccaat cgacattcag cgtacgggag
     1921 tcggcaaggg ctccgattgt tcccgcgaag gcgggaaccc aagcggtgtt tcgggtcctg
     1981 acacgccttt tcggcggcca tcacggcttg ggtccccgcc ttcgcgggga caatcggtga
     2041 gaaatcggcc ccaagtcgtg aatgtcgatt ggccctacgc ctgcatcgtc cagccctcga
     2101 cgcccatggc cgcctggcgc agggcctcgg agcgggtggg gtgcgggtgg caggtgcggg
     2161 cgacgtcctc ggacgaggcc gagaactcca tggccacgca gtattcggcg atcatgtcgc
     2221 cgacgttggg gccgatcatg tgcacgccga ggatgcggtc ggtcttcgca tcggccagca
     2281 ccttcacgaa gccctcgccc tcgtggttga tcttcgcccg gctgttggcg gtgaacggga
     2341 acttgccgac cttgtaggcg acgcccgcgg cct
//
LOCUS       DAORNX010000453         2363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33870_length_2363_cov_6.209705, whole genome shotgun sequence.
ACCESSION   DAORNX010000453 DAORNX010000000
VERSION     DAORNX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33870_length_2363_cov_6.209705"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..453)
                     /locus_tag="PLO65_15105"
     CDS             complement(22..453)
                     /locus_tag="PLO65_15105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD29627.1"
                     /translation="MTWATDRLDALVAGVAEPPPVVTTLRLGTLDAWGEGWARKTWSP
                     SREILNGDGSMFGGYIAALADQIMAFAAMSVAPADAGFRTLNLRVDFMRVGKAEPLVI
                     EGRVTARTKSVIHVEADFRRPDGELIARASAQQMVTPFAAG"
     gene            complement(615..1463)
                     /locus_tag="PLO65_15110"
     CDS             complement(615..1463)
                     /locus_tag="PLO65_15110"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRD29628.1"
                     /translation="MNALAPVIAAPQQAPADHPERQYLGLLADIMTNGVERGDRTGTG
                     TLGVFGRQMRFDLARGFPLLTTKKLHLKSIILELLWFLRGDTNVRWLQERGVSIWNEW
                     ADEAGELGPVYGRQWRSWTAPDGRVIDQISAVVESLKTNPNSRRHIVSAWNPADVDDM
                     ALPPCHCLFQFFVANGRLSCQLYQRSADVFLGVPFNIASYALLTQMMAQVVGLEPGEF
                     VHSFGDAHLYLNHLDQAKEQLRREPLPFPTMRIAPRRDLFAFEYEDFALEGYQAHPGI
                     KAPIAV"
     gene            complement(1532..2086)
                     /locus_tag="PLO65_15115"
     CDS             complement(1532..2086)
                     /locus_tag="PLO65_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRD29629.1"
                     /translation="MSATPIDVIGFWRHAGPDKWFRKDHRFDEAIRLRFEPVHFAAAR
                     GDYDRWMETPDGCLALLLLLDQFPRNLWRNSPHAFASDAMARRVARHMVLSDFDQEIE
                     PALRPFCYLPFEHSEELADQDLSVRLCEALRDATGDEGTLRYAVVHRDIIVRFGRFPH
                     RNRVMGRETTAEEQAFLDEGGFAG"
BASE COUNT          375 a          777 c          871 g          340 t
ORIGIN      
        1 gacttcagca tcggcgcaga cctaacccgc cgcgaacggc gtgaccatct gctgcgccga
       61 ggcgcgggcg atcaactctc cgtcgggccg gcggaagtct gcctcgacat ggatgaccga
      121 cttcgtacgg gcggtcaccc ggccctcgat gacgaggggc tcggccttgc cgacgcgcat
      181 gaaatcgacg cgcaggttca gggtgcgaaa gccggcgtcg gcgggcgcca cggacatggc
      241 ggcaaaggcc atgatctggt cggccagggc ggcgatgtag ccgccgaaca tcgagccgtc
      301 gccgttgaga atttcccgcg atggcgacca ggtcttgcgc gcccagccct cgccccaggc
      361 gtcgagcgtc ccgagccgga gggtggtgac caccggcggg ggttccgcga ccccggcgac
      421 cagggcgtcg agacggtcgg tggcccaggt cacgcggtca ccgggccttg cgggcgtcga
      481 gtcggcggga cgggcgcgcg gcggcggctc cgatcagccc cggggaggag agggatgtcc
      541 atcaggagtc tcctgctgac tggccggccc gagcggcggc gagacggagc caggccgggc
      601 ccgtcgtcac gtggctagac cgcgatcggc gccttgattc ccggatgggc ctggtagccc
      661 tcgagcgcga agtcctcgta ctcgaacgcg aacagatccc gccgcggcgc aatgcgcatg
      721 gtcgggaagg gcaggggctc gcggcgcagc tgttccttcg cctggtcgag gtggttcaga
      781 tacaggtgcg cgtcgccgaa gctgtgcacg aactcgccgg gctcaaggcc gaccacctgg
      841 gccatcatct gggtcagcag ggcgtagctg gcgatgttga acggcacgcc gaggaacacg
      901 tcggcgctgc gctggtagag ctggcagctc agcctgccgt tcgccacgaa gaactggaac
      961 aggcagtggc agggcggcag ggccatgtca tcgacatcgg ccgggttcca ggcgctgacg
     1021 atgtggcggc ggctgttggg gttggtcttc aggctttcga ccacggcgct gatctggtcg
     1081 atcacccggc cgtccggcgc ggtccagctg cgccactggc ggccgtagac cgggcccagc
     1141 tcgccggcct cgtcagccca ttcgttccag atgctgacgc cgcgctcctg cagccagcgg
     1201 acattggtgt ctccgcgcag gaaccacagc agctcgagaa tgatcgactt gaggtgcagc
     1261 ttcttcgtcg tgagcagcgg aaagccgcgc gccaggtcga agcgcatctg ccggccgaag
     1321 acgcccaggg tgccggtgcc ggtgcggtcg ccgcgctcga cgccattggt catgatgtcg
     1381 gccagcaggc cgagatactg ccgctccgga tggtcggccg gagcctgctg aggggcggcg
     1441 atgacgggcg cgagggcgtt catgacgggc ttgagcgtcg ggccgaatca gtcggccgcc
     1501 aagtcacgat cacgaaccgt ccacaggcgg ctcacccggc gaagccgccc tcgtcaagga
     1561 aggcctgctc ctccgccgtg gtctcgcggc ccatcacgcg gttgcgatgg ggaaagcggc
     1621 cgaagcggac gatgatgtcg cggtggacga cggcgtagcg cagcgtgccc tcgtcgcccg
     1681 tggcgtcgcg cagcgcctcg caaagccgca cgctgaggtc ctggtcggcg agctcctcgg
     1741 aatgctcgaa cggcaggtag cagaagggcc gcagggccgg ctcgatctcc tggtcgaagt
     1801 cggacagcac catgtgtcgc gcgacgcgcc gggccatggc gtcgctggcg aaggcgtgcg
     1861 gcgagttgcg ccagaggttg cggggaaact ggtcaagcag cagcagcagc gcgaggcagc
     1921 cgtccggggt ctccatccag cggtcatagt cgccgcgggc cgcggcgaaa tgcaccggct
     1981 cgaaccgcag gcggatggcc tcgtcgaacc ggtggtcctt gcggaaccac ttgtcgggcc
     2041 cggcatgccg ccagaagccg atcacatcga tgggtgttgc gctcatgggc cgaagctggc
     2101 gccgcgcgcg gtttcgttca accgcggttc aggttcggac gcgcatccgt ggttaaaagg
     2161 gactagcagg gaccggcggc gcgccggcct cacggagcag cagatgaaaa agaccatcac
     2221 caccgcgctc gtgctgacgt tgctgggcgg ctccgccgcc tgggccgatc aggactatgg
     2281 ccggcgtggc gcacgccaga acggcggcga ggaatcccgc gcgccggata cgccgccccc
     2341 gccgccgcgg gctcgcgacg acc
//
LOCUS       DAORNX010000454         2361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_33912_length_2361_cov_8.048569, whole genome shotgun sequence.
ACCESSION   DAORNX010000454 DAORNX010000000
VERSION     DAORNX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33912_length_2361_cov_8.048569"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLO65_15120"
     CDS             complement(<1..327)
                     /locus_tag="PLO65_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD29630.1"
                     /translation="MAKDRTGKRGAATEASVGHLLHRAAQRALDFYAEEGGAGGVTQR
                     QHAVLMAVAAREGAAQADLVAATGVDRSTLADMIARMLDKGLLARERSAADARANAVH
                     LTDAGRA"
     gene            complement(439..1035)
                     /locus_tag="PLO65_15125"
     CDS             complement(439..1035)
                     /locus_tag="PLO65_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRD29631.1"
                     /translation="MTGSSARWVRVAVDYGAPIAFAVAFYGFGRDIVLATGVLVAASV
                     LALITGFVVERRIAPLPLIAGIFAVVFGGLTLVFNDASFVKMKITFQNAAFAAALLGG
                     LALGKNPLKALLGESLRMEDAAWRQLTLRYGLYFVAVAVANEVVRNTQSDAFWVNFRT
                     ALLPLAIVFSLSQTPFMMKNLQKPAEGDIAPEPPDPGV"
     gene            complement(1041..1973)
                     /gene="ftsY"
                     /locus_tag="PLO65_15130"
     CDS             complement(1041..1973)
                     /gene="ftsY"
                     /locus_tag="PLO65_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRD29632.1"
                     /translation="MAEQKQGWFQRLTSGLSRTTREMTEQVTSVLVKKPLDQEQLDQL
                     EEMLIEADLGPHAAARITEAFGRARFGKASTEQEVKEALAEAVAAELVPRQGVFDPLS
                     GPRPYVVLFVGVNGSGKTTTLGKIAQDLRLKGAKVLIAAGDTFRAAAVEQLKVWADRA
                     GAAFLSAPIGADSASLAFDAVEKARAEGFDVVLIDTAGRLQNKQDLMNELLKVIRVIK
                     KVDPEAPHETLLVLDATVGRNALAQEKIFGNQVGVSGIVMTKLDGTARGGVLVPVAQA
                     SDSPIKLIGVGEAIEDLQPFDARAFSRSLVGLES"
     gene            2078..>2361
                     /locus_tag="PLO65_15135"
     CDS             2078..>2361
                     /locus_tag="PLO65_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HRD29633.1"
                     /translation="MSPLELGFILFDYAAVAVFGATGALAAARRRHDIITFAFFAAVT
                     GVGGGTLRDLLIEAPVFWVARPGYLVVCLAAAVAVWLLGARTWRMGLLLWL"
BASE COUNT          342 a          826 c          816 g          377 t
ORIGIN      
        1 cgcgcggccc gcatcggtca ggtgcacggc gttggcccgt gcgtcggcgg cggagcgttc
       61 ccgcgccagc agacccttgt cgagcatgcg ggcgatcatg tcggcgaggg tcgagcggtc
      121 gactccggta gccgcgacca gatcggcctg ggccgcgccc tccctggccg cgacggccat
      181 cagcacggcg tgctggcgct gggtgacgcc gccggcgccg ccttcctcag catagaaatc
      241 gagggcgcgc tgggccgcgc gatggagcag gtggccgacg gaggcctccg tcgccgcgcc
      301 ccgcttgccg gtcctgtcct tggccatcgt ctgtctccgg gtccaaccgc ggacggacca
      361 tagtgatcct acacgtcggt ttgacgacag acgatccgta tcgatcctaa tcctctccct
      421 ccgggacagg atttcgacct agacccccgg gtcgggcggc tcgggcgcga tgtcgccctc
      481 ggccggcttc tgcaggttct tcatcatgaa cggggtctgc gacagcgaga acacgatcgc
      541 cagcggcagc agggcggtgc ggaagttgac ccagaaggcg tcgctctggg tgttgcggac
      601 cacctcgttg gccaccgcca cggcgacgaa atacaggcca tagcgcaagg tcagctggcg
      661 ccaggcggcg tcctccatcc gcaggctctc gccgagcagc gccttgagcg ggttcttgcc
      721 cagggccagt ccgcccagca gggccgccgc gaaggcggcg ttctggaagg tgatcttcat
      781 cttcacgaag ctggcgtcgt tgaacaccag cgtcaggccg ccgaacacca ccgcgaagat
      841 gccggcgatc agcggcagcg gggcgatgcg ccgctcgacg acaaagccgg tgatcagggc
      901 cagcaccgag gcggcgacca gcacgccggt ggccagcacg atgtcgcggc cgaagccgta
      961 gaaggccacg gcgaaggcga tcggcgcgcc gtagtcgacg gcgacgcgaa cccagcgggc
     1021 ggatgatccg gtcatggtcg tcagctctcc aggccaacca gcgagcggga aaaggcccgg
     1081 gcgtcgaaag gttgcaggtc ttcgatcgcc tcgccaaccc cgatcagctt gatcggcgag
     1141 tccgaggcct gggcgacggg caccagcacc ccgccgcggg cggtgccgtc cagcttggtc
     1201 atgacaatgc cggagacgcc gacctggttg ccgaagatct tctcctgcgc cagggcgttg
     1261 cgcccgacgg tggcgtccag caccagcaga gtctcgtgcg gcgcctcggg gtcgaccttc
     1321 ttgatcaccc ggatgacctt cagaagctcg ttcatcagat cctgcttgtt ctgcaggcgc
     1381 ccggccgtat cgatcagcac cacgtcgaag ccttcggccc gggccttctc gaccgcatcg
     1441 aacgccaggc tggccgaatc cgccccgatg ggggccgaca ggaaggcggc gcccgcgcgg
     1501 tcggcccaga ccttgagctg ctcgacggcc gcggcgcgga aggtgtcgcc ggcggcgatc
     1561 agcaccttcg cgcccttcag ccgcaggtcc tgggcgatct tgcccagggt cgtggtcttg
     1621 cccgagccgt tgaccccgac gaacagcacg acataggggc ggggtcccga cagcgggtcg
     1681 aacacgccct gacgcggaac cagctcggcg gccacggcct cggccagggc ctccttgacc
     1741 tcctgctcgg tcgacgcctt gccgaagcgc gccctgccga aggcctcggt gatccgcgcg
     1801 gcggcgtggg gcccgagatc ggcctcgatc agcatctctt ccagctggtc gagctgctcc
     1861 tggtcgagcg gcttcttcac cagcacgctt gtgacctgct cggtcatctc gcgggtcgtc
     1921 cgcgacagcc ccgacgtgag gcgctggaac cagccctgtt tctgctctgc catgagcggg
     1981 ctcttagcgg ttccccccag cgccgtcatc gtcggacttg ttccgacgat gacggtggga
     2041 gtgttgaaga ggctccgcac gtggcagaag agcgcacatg tccccgctcg agctcggctt
     2101 catcctgttc gactacgccg ccgtggccgt gttcggcgcc accggcgcgc tggcggccgc
     2161 ccggcggcgg cacgacatca tcaccttcgc cttcttcgcg gcggtcaccg gcgtcggcgg
     2221 cggaacgctg cgcgacctgc tgatcgaagc gccggtcttc tgggtggccc gcccgggcta
     2281 cctggtggtc tgcctcgcgg cggccgtggc ggtctggctg ctcggggcgc ggacgtggcg
     2341 gatgggcctg ctgctgtggc t
//
LOCUS       DAORNX010000455         2355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34043_length_2355_cov_12.453043, whole genome shotgun
            sequence.
ACCESSION   DAORNX010000455 DAORNX010000000
VERSION     DAORNX010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34043_length_2355_cov_12.453043"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="dnaK"
                     /locus_tag="PLO65_15140"
     CDS             <1..1560
                     /gene="dnaK"
                     /locus_tag="PLO65_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRD29634.1"
                     /translation="SAFILMKLKEDAERHLGEKVTKAVITVPAYFNDAQRQATKDAGR
                     IAGLEVLRIINEPTAAALAYGLEKNDGKKIAVYDLGGGTFDVSILEIGDGVFEVKATN
                     GDTFLGGEDFDLRVVDFLADEFKKETGTDLRNDKLALQRLKEEAEKAKKELSTTTQYE
                     VNLPFISMNANGPLHLNIKLSRAKLEALVEDLIAKTIGPCEQALKDAGIKKGDIDEVI
                     LVGGMTRMPKVVDAVKTFFGREPHQGVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTP
                     LTLGIETLGGVFTPLIERNTTIPTKKSQTFSTADDNQSAVTIRVFQGERPMAHDNKML
                     GQFDLVGIPPAPRGVPQIEVIFDIDANGIVHVTAKDKATNKEQSIRIQANGGLSDADI
                     DKMVREAEANRAEDEKRKAGVEARNQADAVIHSTEKALAEHGDKVSADEKAAIETALA
                     DLKSVLDSGDADTIGVKSKALIDASMKLGEAMYKAQQGEAGGDDTARPADDGVVDAEF
                     EEVDGDDKKSA"
     gene            1749..>2355
                     /locus_tag="PLO65_15145"
     CDS             1749..>2355
                     /locus_tag="PLO65_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRD29635.1"
                     /translation="MRDYYDILGVQRGADEAALKAAFRKQAMEHHPDRNGGCEDASAR
                     FKEINEAYSVLSDPQKRAAYDRFGHAGVNGMNGGGGGQGFGDVHDIFNEVFGEAFGEM
                     FGGGRRRQNNGPARGQDLRYDLEITLEQAYAGAEVEITVPASITCESCDGSGAKPGTS
                     PTVCGSCGGAGRVRATQGFFAIERTCPRCGGAGKLVLDPCKD"
BASE COUNT          459 a          794 c          792 g          310 t
ORIGIN      
        1 tcggccttca tcctgatgaa gctcaaggaa gacgccgagc gtcacctggg cgagaaggtc
       61 accaaggcgg tcatcacggt gccggcctac ttcaacgacg cccagcgcca ggcgaccaag
      121 gacgccggcc gcatcgccgg cctggaagtg ctgcgcatca tcaacgagcc gaccgcggcg
      181 gccctggcct acggcctgga gaagaatgac ggcaagaaga tcgccgtcta cgacctgggc
      241 ggcggcacct tcgacgtctc gatcctcgag atcggcgacg gcgtgttcga ggtgaaggcc
      301 accaacggcg acaccttcct cggcggcgag gacttcgacc ttcgcgtcgt cgacttcctg
      361 gccgacgagt tcaagaagga aaccggcacc gacctgcgca acgacaagct ggccctgcag
      421 cgcctgaagg aagaggccga aaaggccaag aaggagctgt cgacgaccac gcagtacgag
      481 gtcaacctgc cgttcatcag catgaacgcc aatggcccgc tgcacctgaa catcaagctg
      541 tcgcgcgcca agctggaagc cctggtcgag gacctgatcg ccaagacgat cgggccgtgc
      601 gaacaggcgc tcaaggatgc cggcatcaag aagggcgaca tcgacgaagt catcctggtc
      661 ggcggcatga cccgcatgcc caaggtcgtc gatgcggtga agaccttctt cggtcgcgag
      721 ccgcaccagg gcgtgaaccc ggacgaggtc gtggcgctgg gcgccgcggt ccaggccggc
      781 gtgctgcagg gcgacgtcaa ggacgtgctg ctgctggacg tgaccccgct gaccctgggc
      841 atcgagacgc tgggcggcgt gttcaccccg ctgatcgagc gcaacaccac gatcccgacc
      901 aagaagagcc agaccttctc gaccgccgac gacaaccagt cggccgtgac gatccgggtc
      961 ttccagggcg agcgtccgat ggcgcacgac aacaagatgc tcggccagtt cgacctggtc
     1021 ggcatcccgc cggcgccgcg cggcgtgccg cagatcgagg tcatcttcga catcgacgcc
     1081 aacggcatcg tgcatgtcac ggccaaggac aaggcgacca acaaggaaca gtcgatccgc
     1141 atccaggcca acggcggcct ctccgacgcg gacatcgaca agatggtccg cgaagccgag
     1201 gccaaccgcg ccgaggacga gaagcgcaag gccggtgtcg aggcgcgcaa ccaggccgac
     1261 gcggtgatcc actccaccga aaaggccctg gccgaacacg gcgacaaggt ctcggccgac
     1321 gagaaggccg ccatcgagac ggccctggcc gatctcaaga gcgtgctgga cagcggcgac
     1381 gccgacacca tcggcgtcaa gtccaaggcc ctgatcgacg cctcgatgaa gctgggcgag
     1441 gccatgtaca aggcccagca gggcgaggcc ggcggtgacg acaccgcccg gcccgcggac
     1501 gacggcgtgg tcgacgccga gttcgaggaa gtcgacggcg acgacaagaa gtcggcgtga
     1561 catcaggcct catggcggcg gaggacggtt ccgtggtgaa aacgcgcccg gggaacgtcg
     1621 ccgccgcctt cgcgaacgcg agaaagtccc gtccgactcg acccgggtcc cctgtggccc
     1681 cggtcttgca gtcggacggg cgatcgccca cctcagacaa tgctcggttt ttttggtgat
     1741 gccggctgat gcgtgactat tacgacatcc tgggcgtcca gcgcggcgcc gacgaggcgg
     1801 cgctgaaggc ggccttccgc aagcaggcga tggagcatca tcccgaccgc aacggcggtt
     1861 gcgaggacgc ctcggcccgc ttcaaggaaa tcaacgaggc ctattcggtc ctgtccgacc
     1921 cgcagaagcg cgccgcctac gaccgcttcg gtcatgccgg cgtcaacggc atgaacggcg
     1981 gcggcggggg ccagggcttt ggcgacgtgc acgacatctt caacgaggtg ttcggcgagg
     2041 ccttcggcga gatgttcggc ggcggtcgcc ggcgtcagaa caacggcccg gcccgcggcc
     2101 aggacctgcg ctacgatctg gagatcaccc tcgagcaggc ctacgccggc gccgaggtcg
     2161 agatcaccgt tccggcctcg atcacctgcg agagctgcga tggctcgggc gccaagccgg
     2221 gcaccagccc gaccgtctgc ggcagctgcg gcggcgcggg ccgggtccgc gcgacccagg
     2281 gcttcttcgc catcgagcgc acctgccccc gttgcggcgg cgccggcaag ctggtgctcg
     2341 acccgtgcaa agact
//
LOCUS       DAORNX010000456         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34076_length_2353_cov_8.132289, whole genome shotgun sequence.
ACCESSION   DAORNX010000456 DAORNX010000000
VERSION     DAORNX010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34076_length_2353_cov_8.132289"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLO65_15150"
     CDS             complement(<1..380)
                     /locus_tag="PLO65_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRD29636.1"
                     /translation="MDTPHPEEDDALLALDPLEIVEHVLAAENLTFDRTDDGDLAFAL
                     AGDWKDYELWFAWRPEADCLQLCLSMGMTTPRAKRASAYELLSLINQRVWLGHFETWA
                     EDGEVVFRHALSLPGGERPTLAAAA"
     gene            complement(506..787)
                     /locus_tag="PLO65_15155"
     CDS             complement(506..787)
                     /locus_tag="PLO65_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRD29637.1"
                     /translation="MQTKNPFLDEIAKLTTAAAGLAQTAGEEAKTAFRAQADRMVAEL
                     DLVRREDLDALKAEIAALRAEVAALKAPRTAAKPAAKPAARPKPGKASE"
     gene            904..1737
                     /gene="lgt"
                     /locus_tag="PLO65_15160"
     CDS             904..1737
                     /gene="lgt"
                     /locus_tag="PLO65_15160"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRD29638.1"
                     /translation="MQFPNFDPVIFHIGPLAIRWYALAYVAGILLGWRYAVGLVRNTR
                     LWGDQRPVITSEQIDDLILWITLGVIVGGRIGYILFYAPNLVWDDPLEIFQVWKGGMS
                     FHGGFIGVVTAIILFAMANRMDMFRLGDIVAPAVPIGLFFGRIANFINGELWGRPTSL
                     PWAIQFPQAGIEYRHPSQLYEAALEGLALFLILRFATHRLMWLQRRGGVAGLFMVGYG
                     LFRISLESVREPDAQMLPFLRNVVTMGTLLSVPMLLFGLWLIWRSRQTPQVVAPEQVE
                     G"
     gene            1737..>2353
                     /locus_tag="PLO65_15165"
     CDS             1737..>2353
                     /locus_tag="PLO65_15165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD29639.1"
                     /translation="MSLMERLRAEIAQTGPINVADYMTRCLHDPQDGYYATRPSLGEA
                     GDFITAPLISQMFGELLGVWAVEAWTRMGRPAAFRLVEMGPGDGTLMSDLLRTVRLAP
                     DFVAAAEVWLIETSWPLRAAQKEKLAGVKPEPNWTGSLGSLPGGLPVILIANELLDCL
                     PARQFVHAEGGWIERLVGLGDDGGLAFGLAPTASGQTPAPDSPVL"
BASE COUNT          329 a          800 c          830 g          394 t
ORIGIN      
        1 gccgccgccg ccagggtcgg gcgctcgccc ccgggcaggc tcagggcgtg gcggaagacc
       61 acctcgccat cctcggccca ggtctcgaag tggccgagcc agacccgctg gttgatcagc
      121 gacagcagtt cgtaggccga ggcgcgcttg gccctgggcg tggtcatgcc catggacagg
      181 cacagctgca ggcagtcggc ttccggccgc caggcgaacc acagctcgta gtccttccag
      241 tcgccggcca gggcgaaggc gaggtcgccg tcgtccgtcc ggtcgaaggt caggttctcg
      301 gcggcgagca cgtgctcgac gatttcgagc ggatcgagcg ccagcagggc gtcgtcttct
      361 tcgggatggg gcgtgtccat agggcctctg tatccctccg acctgtggcc gggcggggaa
      421 gcgcccggcg gaagcagcgt ccttgaacca ggagcctaga ctgcttcgcc gtgggcgtaa
      481 ggggtgttct tgccgggcgc gagccctact cgctcgcctt gcccggtttg ggcctggcgg
      541 cgggcttggc cgccggcttg gcggcggttc gcggagcctt gagcgccgcc acctcggccc
      601 gcagggcggc gatctcggcc ttgagcgcgt cgagatcctc gcggcggacg agatccagct
      661 cggcgaccat gcggtcggcc tgggcgcgga aggcggtctt cgcctcctcg cccgctgtct
      721 gggcaaggcc ggcggccgcc gtggtcagct tggcgatctc gtcgagaaag gggttcttgg
      781 tctgcatcgg ggccctcggg gacgcgaaat ggcgcggccg tcgcccctaa tatgggaaac
      841 cgggggtgaa agcgaagggg atcgcagtta tcacaggcgc cagacgctcc tccggagaga
      901 aacgtgcagt ttccgaactt cgaccccgtc attttccaca tcggcccgct ggccatccgc
      961 tggtacgccc tggcctacgt cgccggcatc ctgctcggct ggcgctacgc cgtcggcctg
     1021 gtgcgcaaca cccggctgtg gggcgatcaa aggcccgtga tcaccagcga acagatcgac
     1081 gacctgatcc tgtggatcac cctgggcgtc attgtcggcg gccggatcgg ctacatcctg
     1141 ttctacgcgc ccaacctggt ctgggacgat ccgctggaaa tcttccaggt ctggaagggc
     1201 ggcatgagct tccacggcgg cttcatcggg gtggtcacgg ccatcatcct gttcgccatg
     1261 gccaaccgca tggacatgtt ccggctgggc gacatcgtcg cgccggcggt gccgatcggg
     1321 ctgtttttcg ggcgcatcgc caacttcatc aacggcgaac tgtggggccg gccgaccagc
     1381 ctgccctggg ccatccagtt cccgcaggcc ggcatcgagt atcgccatcc cagccagctg
     1441 tacgaggccg cgctggaagg cctcgccctg ttcctgatcc tgcgcttcgc cacccaccgg
     1501 ctgatgtggc tgcaacgccg cggcggggtg gccgggctgt tcatggtcgg ctacggcctg
     1561 ttccgcatca gtctggagag cgtgcgcgag cccgacgcgc agatgctgcc cttcctgcgc
     1621 aacgtggtca ccatgggcac gctgctgtcc gtcccgatgc tgctgttcgg cctgtggctg
     1681 atctggcggt cgcggcagac gccccaggtc gtcgcgccgg agcaggtcga aggctgatga
     1741 gcttgatgga acgcctgcgc gccgagatcg cgcagaccgg cccgatcaac gtggccgact
     1801 acatgacgcg ctgcctgcac gatccgcagg acggctacta cgccacccgc ccctcgctgg
     1861 gcgaggccgg cgacttcatc accgcgccgc tgatcagcca gatgttcggc gagctgctgg
     1921 gcgtctgggc ggtcgaggcc tggacccgca tgggcaggcc ggcggccttc cggctggtcg
     1981 agatgggtcc cggcgacggc accctgatga gcgacctgct gcgcaccgtg cgactggcgc
     2041 ccgatttcgt cgccgccgcc gaggtatggc tgatcgaaac ctcctggccg ctgcgggcgg
     2101 cgcagaagga gaagctggcc ggggtgaagc ccgagccgaa ctggacgggg tccctcggca
     2161 gcctgcccgg cggactgccg gtaatcctta tcgcaaacga gttgctcgac tgcctgccgg
     2221 cgcggcagtt cgtccacgcc gagggcggct ggatcgaacg gctggtcggc ctcggcgacg
     2281 acggcggact ggcctttggc ctggccccga ccgccagcgg ccagacgccg gcgccggaca
     2341 gcccggttct gga
//
LOCUS       DAORNX010000457         2351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34120_length_2351_cov_11.320993, whole genome shotgun
            sequence.
ACCESSION   DAORNX010000457 DAORNX010000000
VERSION     DAORNX010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34120_length_2351_cov_11.320993"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..551)
                     /gene="mmcB"
                     /locus_tag="PLO65_15170"
     CDS             complement(120..551)
                     /gene="mmcB"
                     /locus_tag="PLO65_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair putative endonuclease MmcB"
                     /protein_id="HRD29640.1"
                     /translation="MDVALTFITVSRPDTTVAVTRGAARLLTEMTYAPLAEVGLPNGR
                     RADLMALGPKGEIVIVEVKSGPEDYLTDRKWAEYAPYCDAFFFAVSPGFPLDLIPPEP
                     GLIVADGFGGAIVREPGWTPLVAARRKALTLAFARLAAFRG"
     gene            complement(583..1107)
                     /locus_tag="PLO65_15175"
     CDS             complement(583..1107)
                     /locus_tag="PLO65_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRD29641.1"
                     /translation="MKRTSLLAVAAGLAIAGVAWAAAPQPTRPVSTSFYSGRWYEIAR
                     IPNAGQRDCQAPYSQFVSTGGAAFKVSQICHRGSANGPAKAFSTTGRILPGTNAAKFE
                     MSFFGGIRKQEYWVLDTAGDGAWAIMATPGGNYVWLMAREAVMDPSAKAAVVARIRSL
                     GYRQRLEFPAQRAG"
     gene            complement(1225..>2351)
                     /locus_tag="PLO65_15180"
     CDS             complement(1225..>2351)
                     /locus_tag="PLO65_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRD29642.1"
                     /translation="SDKNITKDAMYDAVAPDDFESMLELDRYNNRSTAFDKIISATHD
                     HFWDPLDKEYIDFDEPFDMVNQAIVPEDMVVALSTDYVSNHLSDPLERIRFINESTLR
                     SFSSILHGEQGALNLSASLCHVLKDQGAQEYAANQTREEARHVTAFAKYVKARWGRPV
                     ECGPTLKDLLVEIIGAPEVYKKIIGMQMLVEGLAMGAFATFYNKLNDPLGKKLMQLVM
                     TDEAFHHKFGKIWADRTIPKLSPEEHAVIEDWAAHCFQAVLFNLVGPNQQRDLYESFG
                     LDPDKVVAEFAKIMTDEQRREGMKEQTNIFRVLVKTLLNAGIITDRTKAFYGMYVDLE
                     ELKTEGDRMVGDDIAEDGIRYLQAINFKDREHKPVSIAAE"
BASE COUNT          357 a          760 c          813 g          421 t
ORIGIN      
        1 cagcggattc tggccaagcg cgcgccgcgc tagccagccg gggacgtgca cttcagtgcg
       61 tgtccccatc cgctggggag cggcagggga cacgcactga agtcggtgca tgtccccgat
      121 caccccctga acgccgccag ccgcgcgaag gccagggtca gcgccttgcg ccgcgccgcg
      181 accagcggcg tccagccggg ctcgcgcacg atcgccccgc cgaacccgtc ggcgacaatc
      241 agtcccggct cgggcggaat caggtcgagc gggaatcccg gcgacacggc gaagaagaag
      301 gcgtcgcagt agggggcgta ctcggcccat tttcggtcgg tcagatagtc ctccggcccc
      361 gacttgacct cgacgatgac gatctcgccc ttgggtccca gcgccatcag gtccgcccgc
      421 cgaccgttcg gcagtccgac ctcggccagc ggcgcatagg tcatctcggt cagcagccgc
      481 gccgccccgc gtgtcaccgc gacggtggtg tccggccggg agaccgtgat gaaggtgagc
      541 gcgacgtcca cggccgcatt ggtgttccgc tttcgttcgg tgtcaacccg cccgctgggc
      601 cggaaactcc agccgctggc gatagccgag ggaacggatg cgcgcgacga ccgcggcctt
      661 cgccgacggg tccatgaccg cctcgcgcgc catcagccag acgtagttgc ctccaggggt
      721 ggccatgatc gcccaggcgc cgtcgccggc ggtgtcgagg acccagtatt cctgcttccg
      781 tatcccgccg aagaagctca tctcgaactt ggcggcgttg gtcccgggca ggatccggcc
      841 cgtggtcgag aaggccttcg ccggcccgtt ggccgagccc cggtgacaga tctggctgac
      901 cttgaaggcc gcgccgcccg tgctgacgaa ctggctgtag ggcgcctggc agtcgcgctg
      961 cccggcgttg ggaatgcggg cgatctcgta ccagcgcccg gaatagaagc tggtcgagac
     1021 ggggcgggtc ggctgcgggg cggcggccca ggcgacgccg gcgatcgcca gacccgccgc
     1081 gacggcgagg agggacgtgc gtttcatctg gtcaagtcta gcgtctcgga cggaaaaggg
     1141 gactccgccg aaccgcggtc tgaaacgcag aacgccccgc cgggttgcgg cggggcgttc
     1201 gcgggtccgt ccggggaggg atgactactc ggcggcgatg ctgaccggct tgtgttcgcg
     1261 gtccttgaag ttgatcgcct gcagatagcg gatgccgtcc tcggcgatgt catcgccgac
     1321 catgcggtca ccctcggtct tgagctcctc gaggtcgacg tacatgccgt agaaggcctt
     1381 ggtccggtcc gtgatgatcc cggcgttgag cagcgtcttg accagcacgc ggaagatgtt
     1441 ggtctgctcc ttcatgccct cgcgacgctg ctcgtcggtc atgatcttgg cgaactcggc
     1501 gaccaccttg tcggggtcga ggccgaagct ctcgtacagg tcgcgctgct ggttggggcc
     1561 gaccaggttg aacagcaccg cctggaagca gtgggcggcc cagtcctcga tcaccgcatg
     1621 ttcctcgggg ctgagcttgg ggatcgtgcg atcggcccag atcttcccga acttgtggtg
     1681 gaaggcctcg tcggtcatga ccagctgcat cagcttcttg ccgagcgggt cgttgagctt
     1741 gttgtagaag gtggcgaagg cgcccatcgc cagaccctcg accagcatct gcatgccgat
     1801 gatcttcttg tagacctccg gcgcgccgat gatctcgacc agcaggtcct tcagggtcgg
     1861 gccgcactcg accggacggc cccagcgcgc cttgacgtat ttggcgaagg ccgtgacgtg
     1921 gcgggcttcc tcgcgggtct ggttggcggc gtattcctgc gcgccctggt ccttcagcac
     1981 gtggcagagg ctggccgaca ggttcagcgc gccctgttcg ccatgcagga tcgaggagaa
     2041 gctgcgcagg gtcgactcgt tgatgaagcg gatgcgctcc agcgggtcgg acaggtggtt
     2101 ggagacgtag tccgtcgaca gcgccacgac catgtcttcc ggcacgatgg cctggttgac
     2161 catgtcgaac ggctcgtcga agtcgatgta ctccttgtcc agcggatccc agaagtgatc
     2221 gtgggtcgcc gagatgatct tgtcgaacgc cgtggagcgg ttgttgtagc ggtcgagctc
     2281 gagcatcgac tcgaagtcgt ccggcgccac cgcgtcgtac atggcgtcct tggtgatgtt
     2341 cttgtcgctc a
//
LOCUS       DAORNX010000458         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34145_length_2350_cov_4.601743, whole genome shotgun sequence.
ACCESSION   DAORNX010000458 DAORNX010000000
VERSION     DAORNX010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34145_length_2350_cov_4.601743"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    276..337
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <338..908
                     /locus_tag="PLO65_15185"
     CDS             <338..908
                     /locus_tag="PLO65_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRD29643.1"
                     /translation="TGVGLLAVYAALKARDRNGWLLTAVMTLGFLGDVLLGIQFIVGA
                     VAFLVGHVMAIGLYLTNRRPAPTRSQTALALLLVPASVFIAFLLPADRAAAPGVAFYT
                     LGLSLMAATAWTSRFPRFTVGIGALLFLVSDLVIFAKMGPLPDTFLTGLAVWVPYYVG
                     QLLICVGVVAALAESGHVPPSVRVPETSA"
     gene            complement(943..>2350)
                     /locus_tag="PLO65_15190"
     CDS             complement(943..>2350)
                     /locus_tag="PLO65_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD29644.1"
                     /translation="VSPQYGLAGADLSRLAYACGVLSPDAVYADDAAAFGGALAADFL
                     QGLPVVASTNARAGDIMFADLLKSPPTDFVARPDDTAKLLLTSGSTGRPKAVIGLHRN
                     IAFNAAQIAACFDDPDPPVVVNAAPWSHSLGANAILHMVLHRGGTLYIDAGQPTPGRF
                     AETVRNLREVSPTYHNMVPAGWALFASELEKDVDLARTFFKRVRVLQYGGASIAQSII
                     DRVQAVAIATIGERVTFATGYGSTETGPTACNIHWLNGRGGMIGLPVPGTAVKLVPAG
                     EKFEIRVKGPQVSPGYLGEPGLSAEAFDEEGFYRLGDAARAIDPADPEAGLMFDGRLV
                     ENFKLASGTFVAAGALRVTAVSAIGGAVSDAVVCGEGQEGVGLMLFLDPGFNARHPDP
                     AEVRAVIRAGLERMNAAAKGGGGRIWRALILDGAPDAASGELTDKGYINQALARDRRP
                     AELARLFAEAPDDGVMVF"
BASE COUNT          298 a          850 c          781 g          359 t
ORIGIN      
        1 ggcctcggcc ccggtcagga cgccgcccgg cgccagcgcg gctcgcaggt ccgcgatcaa
       61 ctcgtccatg ccgccctccc tggcgttgtc ccggtcaggc tagcgtccgg cgccagcgtc
      121 cggattgatc cagaccaatc caccgcccgc gagtccgggc cgggggcttt tttcgccccg
      181 gcaaaccggg cattcagagg cgggacaggg ggacatcatg acactgcgcg acagggccgc
      241 ccgctgggcg ctgaccggtt cgatcatcgg cggggnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac cggcgtcggc ctgctggccg
      361 tgtacgccgc cctcaaggcc cgcgaccgga acggctggct gctgacggcg gtgatgacgc
      421 tcgggttcct cggcgacgtt ctgctgggca tccagttcat cgtcggcgcg gtggccttcc
      481 tggtcggcca cgtcatggcg atcggcctct acctgaccaa ccgccgcccc gccccgaccc
      541 gcagccagac ggcgctggcc ctgttgctgg tccccgcctc ggtgttcatc gccttcctgc
      601 tgccggccga ccgcgccgcc gcgccggggg tggccttcta cacgctgggc ctgtcgctga
      661 tggccgccac cgcctggacc agccgcttcc cgcgcttcac ggtcgggatc ggcgcgctgc
      721 tgttccttgt cagcgacctg gtgatcttcg ccaagatggg cccgctgccg gacaccttcc
      781 tcaccggcct ggcggtctgg gtcccctact atgtcggcca gctgctgatc tgtgtggggg
      841 tggtggcggc gttggccgaa agtggacatg tacctccttc ggtacgtgtc cccgaaacca
      901 gcgcctgatc ggggacacgt acctgcggta catgtcccct acctagaaca ccatcacccc
      961 gtcatccggc gcctcggcga acagccgcgc cagttcggcc ggccggcggt cccgcgccag
     1021 ggcctggttg atgtagccct tgtcggtcag ctcgccgctg gccgcgtcgg gcgcgccgtc
     1081 gaggatcagg gcccgccaga tccggccgcc gccgcccttc gccgccgcgt tcatcctttc
     1141 caggccggcg cggatcaccg cccgtacttc ggccggatcg ggatgccttg cgttgaaccc
     1201 cgggtcgagg aacagcatca ggccgacgcc ctcctgcccc tcgccgcaga ccaccgcgtc
     1261 gctgaccgcg ccgccgatgg ccgagacggc ggtcacgcgc agggcgcccg ccgcgacgaa
     1321 cgtgccgctg gccagcttga agttctccac cagccggccg tcgaacatca ggcccgcttc
     1381 cggatcggcc ggatcgatgg cccgcgccgc gtcgcccagg cgatagaagc cttcttcgtc
     1441 gaaggcctcc gccgacaggc cgggctcgcc cagatagccg ggcgagacct gcggcccctt
     1501 cacgcggatc tcgaacttct cgccggccgg gaccagcttc accgccgtgc ccggcaccgg
     1561 caggccgatc atcccgcccc ggccgttcag ccagtggatg ttgcaggccg tcgggccggt
     1621 ctcggtcgaa ccatagcccg tggcgaaggt cacgcgttcg ccgatggtgg cgatggccac
     1681 ggcctggacc cggtcgatga tgctctgggc gatcgaggcg ccgccgtact gcagcacccg
     1741 cacccgtttg aagaaggtcc gcgccaggtc aacgtccttc tccagttcgc tggcgaacag
     1801 cgcccagccg gccggcacca tgttgtggta ggtcggcgag acctcgcgca ggttgcgcac
     1861 ggtctcggcg aaccgcccgg gcgtcggctg gccggcgtcg atgtacagcg tcccgccccg
     1921 gtgcagcacc atgtgcagga tggcgttggc ccccaggctg tggctccagg gcgcggcgtt
     1981 gaccacgacg ggcgggtcgg gatcgtcgaa acaggcggcg atctgggcgg cgttgaaggc
     2041 aatgttgcga tgcaggccga tgaccgcctt gggccgtccg gtcgagccgg aggtcagcag
     2101 cagcttggcc gtgtcgtccg gtcgcgcgac gaagtccgtc ggcggggatt tcagcaggtc
     2161 ggcgaacatg atgtcgccgg cgcgcgcgtt cgtgctggcc accaccggca ggccctgcag
     2221 gaagtccgcc gccagcgccc ccccgaaggc cgccgcgtcg tcggcgtaga ccgcgtcggg
     2281 gctcagcacg ccgcaggcgt aggccagccg cgacagatcg gctccggcca ggccgtactg
     2341 cggcgacacc
//
LOCUS       DAORNX010000459         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34209_length_2346_cov_7.321694, whole genome shotgun sequence.
ACCESSION   DAORNX010000459 DAORNX010000000
VERSION     DAORNX010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34209_length_2346_cov_7.321694"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..2244
                     /gene="aspS"
                     /locus_tag="PLO65_15195"
     CDS             463..2244
                     /gene="aspS"
                     /locus_tag="PLO65_15195"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRD29645.1"
                     /translation="MHAYRTHTCGQLRASDTGAAVRLSGWIHRKRDHGGLVFVDLRDH
                     YGLTQLVVGPTTPGFDLIERIRTESVIRVDGSVVARSAETINPNLPTGEVEIHVSGVE
                     ILSEAAELPLPVFGEPDYPEELRLKHRYLDLRRETLHRNIVLRSKVIHSIRHRMVGQG
                     FLEYQTPILTASSPEGARDFLVPSRLHASKFYALPQAPQQFKQLLMVSGFDRYFQIAP
                     CFRDEDLRADRSLEFYQLDVEMSFVTQEDVFAAIEPVMHGVFEEFANGKPVTPYPFPR
                     IAYADAIAWYGSDKPDTRNPIRMRDVSDSFRGGGFGLFARILDADAKNAVWAVPAPGG
                     GSRAFCDRMNSWAQGEGQPGLGYVFWSEDQGGWGGPIAKNLGAEPTQALMDGLGLGQG
                     DAAFFVAGDPKVFYKFAGSARTRVGTELKLIDEDRFEFCWIVDFPMFEWSDEEKKVDF
                     SHNPFSMPQGGMQALETQDPLSILAYQYDIVCNGYELCSGAIRNHKPEIMLKAFEIAG
                     YGADVVEAQFGGMLNAFRYGAPPHGGLAPGIDRIVMLLAGQTAIREVIAFPLNQQGQD
                     LLMSAPSEVSDKQLKELHIRLAPPIKG"
BASE COUNT          371 a          840 c          776 g          359 t
ORIGIN      
        1 cccgcctgtg gacggatggg tcgcagcgac gagactcacg ggacggatag agtcgggaca
       61 acgccgggac tcccgaccct ctcgcggaga ggagagcggc cccggccccc ggcgacgggc
      121 gatgagggct tgcggcgtcg accggcggat tccggacgcc ctcaactccc tgcggctccg
      181 cggggccgcc ctccctccgt ctcaccgcgc cggaggggcc tggggcgccc tatcccgctg
      241 aattcattgg ggccgacgac tgttccgcgg ccctgttccg ccaggcgttg ccatgaccgt
      301 cgaggagacg cgcccgtcgt cgcgtcggct ctcttcctca acggcgccgg aagccgaaac
      361 ggaaagcagg actcccgccc ctgaaccttg acaatccgca ccccccatgc gcccttccgc
      421 gcctccgaaa tttgcgcttc ggcgcctgaa aacgacctga aaatgcacgc ctatcgcacc
      481 cacacctgcg gccagctccg cgcttccgac accggcgccg cggtgcgcct gtccggctgg
      541 atccaccgca agcgcgacca cggcggcctg gtcttcgtcg acctgcgcga ccactacggc
      601 ctgacccagc tggtcgtggg gccgaccacg ccgggcttcg acctgatcga gcgaatccgc
      661 accgagagcg tcatccgggt cgacggctcg gtcgtcgcgc gctcggccga gaccatcaac
      721 cccaacctgc cgacgggcga ggtcgagatt cacgtctccg gcgtcgagat tctgtcggag
      781 gcggccgagc tgcccctgcc ggtgttcggc gagcccgact atcccgagga gctgcgcctg
      841 aagcaccgct acctcgacct gcggcgcgag accctgcacc ggaacatcgt gctgcggtcg
      901 aaggtcatcc actcgatccg tcaccgcatg gtcggccagg gcttccttga gtaccagacg
      961 ccgatcctga cggcctcctc gccggagggc gcgcgcgact tcctcgtgcc ctcgcgcctg
     1021 catgcgtcga agttctacgc gctgccgcag gcgccgcagc agttcaagca gctgctgatg
     1081 gtctccggct tcgaccgtta cttccagatc gcgccctgct tccgtgacga ggacctgcgc
     1141 gccgaccgca gcctggaatt ctaccagctc gacgtcgaga tgagcttcgt cacccaggaa
     1201 gacgtgttcg cggccatcga gccggtcatg cacggggtgt tcgaggagtt cgccaacggc
     1261 aagccggtca cgccctatcc gttcccgcgc atcgcctacg cagacgccat cgcctggtac
     1321 ggctccgaca agcccgacac ccgcaacccg atcaggatgc gcgatgtctc ggacagcttc
     1381 cgtggcggcg gcttcggcct gttcgcccgg atcctcgacg ccgacgcgaa gaacgccgtc
     1441 tgggccgtgc cggcgccggg cggcggctcg cgcgccttct gcgaccggat gaacagctgg
     1501 gcccagggcg aaggccagcc gggcctcggc tatgtgttct ggagcgagga ccagggcggc
     1561 tggggcggtc cgatcgccaa gaacctcggc gccgagccga cccaggctct gatggacggc
     1621 ctcggcctcg gccagggcga cgcggccttc ttcgtcgcgg gtgacccgaa ggtgttctac
     1681 aagttcgccg gttcggcccg cacccgcgtg gggacggagc tgaagctgat cgacgaggat
     1741 cgcttcgagt tctgctggat cgtcgacttc ccgatgttcg agtggagcga cgaggagaag
     1801 aaggtcgact tctcccacaa ccccttctcg atgccgcagg gcggcatgca ggcgctggag
     1861 acccaggacc cgctgtcgat cctcgcctac cagtacgaca tcgtctgcaa cggctacgag
     1921 ctgtgctcgg gcgcgatccg caaccacaag cccgagatca tgctcaaggc gttcgagatc
     1981 gccggctatg gcgccgatgt cgtggaggcg cagttcgggg gcatgctcaa tgcgttccgt
     2041 tacggcgcgc cgccgcacgg cggcctggcc cccggcatcg accggatcgt catgctgctg
     2101 gccggccaga cggcgatccg cgaggtgatc gccttcccgc tgaaccagca ggggcaggat
     2161 ctgttgatga gcgcgccctc agaagtcagc gacaagcagc tgaaagaact gcacatccgg
     2221 ctggcgccgc ccatcaaggg ctgacgtgga cggcggcctc agccgcgaac ggcgatgatc
     2281 tgggaaggtc cggccgcgcg cggcggggct ggcggagcag gcggcgccgg aggggccgcc
     2341 cgaacc
//
LOCUS       DAORNX010000460         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34433_length_2334_cov_5.037297, whole genome shotgun sequence.
ACCESSION   DAORNX010000460 DAORNX010000000
VERSION     DAORNX010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34433_length_2334_cov_5.037297"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..905
                     /locus_tag="PLO65_15200"
     CDS             246..905
                     /locus_tag="PLO65_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29646.1"
                     /translation="MTLRLFAHPLSSYCWKALIPLYEAGTPFSFELIQFGAGDGPVVE
                     FMQRWPVGKMPLLRDEARDQTVAESSIIIEYLDRHYPGAGLIPADPDAALTARFMDRF
                     FDNYVMTPQGRLVFEVIRPEGSKDPFGVAEARALLDKAYAWLEETLSDGRTWAAGETY
                     SLADVSASPSLFYAQRCHPWGDRPALQAYYDRLETRPSFQRVIQEAQPYMHMFPGGPG
                     Y"
     gene            complement(1006..>1591)
                     /locus_tag="PLO65_15205"
     CDS             complement(1006..>1591)
                     /locus_tag="PLO65_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD29647.1"
                     /translation="KRATTHWNRSKDFARRYPKVKLEPDRIWVRDGAVWTSAGITAGI
                     DLALALIAEDLGEDIARKTAQQLVVYHRRPGGQSQFSALLELEAGGRFDSLMAWARES
                     LRHPLTVAELADRAGMSERNFARAFAAATGFTPAKAIERLRVEAARTLLDSQPLQVED
                     VALETGFGDAERMRRAFIRAFGQPPQALRRARAA"
     assembly_gap    1592..1608
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1609..1953)
                     /locus_tag="PLO65_15210"
     CDS             complement(<1609..1953)
                     /locus_tag="PLO65_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRD29648.1"
                     /translation="MRRIGVLAFRDFQLLDAAGPIGAFEIAGRYVPGAYDIRVLSRDG
                     GRVASSSGVTLDSLSFAEAGPLDTLLVSGGEGTRGPSIDAGTLAWVRQTHRTARRTAS
                     VCSGAYVLAQAGL"
     gene            2033..>2334
                     /locus_tag="PLO65_15215"
     CDS             2033..>2334
                     /locus_tag="PLO65_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRD29649.1"
                     /translation="MTAESLRIVFALYPGVTHLDFTGPHQFLSRLPGAVTTVASLDGG
                     EMEADGLTFARLTRFADIDSCDLLCIPGGFGTTEAMLHEPFMAQIRRLAEVAQLCD"
BASE COUNT          338 a          796 c          816 g          367 t
ORIGIN      
        1 tccctctctc aaccgctcat cccggcgaag gccgggaccc aggcgttttt gaagccgccg
       61 ctcacggttc ggattggcta catggcctgc ctgaacggtg agccgaagct ctgaaaaaac
      121 ctgggtcccg gccttcgccg ggatgagcgg atttcatgac atcgttgtcc gcagggacat
      181 gcggttgacc gttcgcgccc gcaacactaa agtaattgct taactgttgc cgcgagcccc
      241 cgcccatgac cctccgcctc ttcgcccacc cgctgtcgtc ctactgctgg aaggcgctga
      301 tcccgctcta tgaggccggg acgccgttca gcttcgagtt gatccagttc ggggccgggg
      361 acgggccggt ggtcgagttc atgcagcgct ggccggtcgg caagatgccg ctgctgcgcg
      421 acgaggcgcg ggatcagacg gtcgcggagt ccagcatcat catcgagtac ctcgaccgcc
      481 actatccggg cgcgggcctg atcccggccg atccggacgc ggcgctgacg gcgcggttca
      541 tggaccggtt cttcgacaac tatgtcatga cgccccaggg ccggctggtg ttcgaggtca
      601 tccgccccga gggctccaag gacccgttcg gcgtggccga ggcgcgggcc ctgctcgaca
      661 aggcctatgc ttggctggag gagaccctgt ccgacggccg gacctgggcg gccggcgaga
      721 cctattccct ggccgacgtc agcgccagcc cgtcgctgtt ctacgcccag cgctgccacc
      781 cctggggcga ccgcccggcg ctgcaggcct actacgaccg cttggagacg cggccgtcct
      841 tccagcgcgt gatccaggag gcgcagccgt acatgcacat gttcccaggc gggccggggt
      901 actgaggggc tggggagtag ggagtaggga gtagggagta gggagtagac gcgggggtgg
      961 cggcggctgg cggcgccttg ctagccccta gctactccct actccctacg ccgcccgcgc
     1021 gcgccgcagc gcctgcgggg gttgcccgaa ggcgcggatg aaggctctcc gcatgcgctc
     1081 tgcgtctccg aagccggttt ccagggccac gtcctcgacc tgcagcggct gggaatccag
     1141 cagggtgcgg gcggcctcga cccgcaggcg ttcgatggcc ttggccgggg tgaagccggt
     1201 ggcggcggcg aaggcgcggg cgaagttgcg ctcgctcatg cccgcgcggt cggccagttc
     1261 ggccacggtc agcgggtggc gcaggctttc ccgcgcccag gccatcaggc tgtcgaaccg
     1321 gccgccggcc tcgagctcga gcagggccga gaactgcgac tggccgccgg ggcggcggtg
     1381 gtagacgacc agctgctggg ccgtcttgcg ggcgatgtcc tcgcccaggt cctcggcgat
     1441 cagggccagg gccaggtcga tgccggcggt gatccccgcg ctggtccaga ccgcgccgtc
     1501 gcggacccag atgcggtccg gctccagctt caccttcgga tagcggcggg cgaaatcctt
     1561 gctgcggttc cagtgggtgg tagcgcgttt tnnnnnnnnn nnnnnnnnca ggccggcctg
     1621 ggccagcaca taggcgccgg agcagacgct ggcggtgcgc cgggccgtgc ggtgggtctg
     1681 gcgaacccag gcgagcgtgc ctgcgtcgat ggaggggccg cgcgtgccct cgccgccgga
     1741 gaccagcagg gtgtcgagcg ggccggcctc ggcgaacgac aggctgtcca gcgtcacgcc
     1801 cgacgagctg gccacccggc cgccgtcccg cgacagaacc ctgatgtcgt aggcgcccgg
     1861 cacatagcgg ccggcgatct cgaacgcgcc gatcggcccg gccgcgtcga gcagctggaa
     1921 gtcgcggaag gcgaggacgc ctatccggcg catggcggaa aacgaggtaa tattgtcatt
     1981 tcggacggac gctaggaccg gcaggatggt cgcgtcaacc ggagcccgcc tcatgaccgc
     2041 cgaatccctg cgcattgtct tcgccctcta tcccggagtc acccacctcg acttcaccgg
     2101 accgcaccaa ttcctgtccc gcctgcccgg cgccgtgacc accgtggcca gcctcgacgg
     2161 cggcgagatg gaggccgacg gtctgacctt cgcccggctc acgcggttcg cggatatcga
     2221 cagctgcgac ctgctctgca ttcccggcgg gttcggcacg accgaggcca tgcttcatga
     2281 gcccttcatg gcgcaaatcc ggcggctggc cgaggtggcg caactctgcg acga
//
LOCUS       DAORNX010000461         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34645_length_2322_cov_7.679753, whole genome shotgun sequence.
ACCESSION   DAORNX010000461 DAORNX010000000
VERSION     DAORNX010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34645_length_2322_cov_7.679753"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..784)
                     /locus_tag="PLO65_15220"
     CDS             complement(170..784)
                     /locus_tag="PLO65_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29650.1"
                     /translation="MKLYWHPVSLMPWRVRIALAEKGLAYEEVMIDLPGGESHSPEFL
                     VLNPFGQVPVLEDDGRAFAESTAILEYLEESHPRPALMPAAAAARAEVRQLMCWSNGP
                     WMMSWKRWVGPASLGFPDTEDVRTQGRAELEHHLTLLVARLERSAWLAGDYSLADICY
                     APLVLMAGRMGLSAEGPGQAAIDGWLDRLRARPAVQAAMGPILG"
     gene            complement(781..>2322)
                     /locus_tag="PLO65_15225"
     CDS             complement(781..>2322)
                     /locus_tag="PLO65_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRD29651.1"
                     /translation="GLPKGSNGVAVAPSRSSDGATRLLVNSHQPYSGPVAWYEAVVES
                     GEGWHVAGGFFPGSPFMLHGHNAHLGWANTVNSPDLVDIYRLTINPADEDEYRLDGKW
                     RKFEKSTARLRVRLWGPFSWTVNKEVLRSVHGPVMRTKGGVFAIRYAGMDTVRQPLQY
                     WRLDKAASIDEWRAAMALQALPSINYVYADEKGNIGYVYNGLFPNRREGVDWSGVLPG
                     DRSDLIWKGYLPFDRVPQVWRPKGGLVFNANNTPFQATAAGDGLDPWAFSPTMGIQTN
                     MTNRSLREVETFAADDQITLEEFRAYKFDVTYSHRSDVAAFVGELLGLDPKGDADIAA
                     AQAILARWDYTTRVTSRGAALAILPAQIVLTARDSHDPKAPTARQALIDTITLLRRHF
                     GRLDPEWGQVNRIRRGSVDLPIDGGPDTLRAVYGKPADDGRLAAVAGDTFIMFVTWDR
                     DGVLSSQSIHQFGAATLDASAPHYSDQVPLFAAMRFKPVLFTEAQLAGNVVETYRPGQ
                     RGAAP"
BASE COUNT          356 a          773 c          827 g          366 t
ORIGIN      
        1 gctcgcgttc ttcggcgagc acggcggggt cggcgtagac gaagccgggc agggcgcgcg
       61 cggcctccag ccgcgtcgcc accgtcgcat agggatttcc ggaccgcgtc tggctcatag
      121 cgaggccgac gctaacgatc tcgcggccgg ccgcaaccgt gacccggcgt cagccgagga
      181 tcggacccat ggcggcctgg acggccgggc gcgcgcggag tcgatccagc cagccgtcga
      241 tcgcggcctg acccggtcct tcggccgaca ggcccatgcg gccggccatc agcaccaggg
      301 gggcgtagca gatgtccgcg agggaataat cgccggccag ccaggctgac cgctcgagcc
      361 gggcgaccag gagggtgaga tggtgttcga gctcggcgcg gccctgggtt cgcacgtctt
      421 cggtgtccgg aaagccgagg gaggcgggcc ccacccagcg tttccacgac atcatccagg
      481 ggccgttgct ccagcacatc agctggcgaa cctccgcccg cgcggcggcg gcggccggca
      541 tcagggccgg tcgggggtgg ctctcctcaa ggtattcgag gatcgcggtg gactcggcga
      601 aggcccgtcc gtcgtcttcc agcaccggaa cctgcccgaa cgggttgagg acgaggaatt
      661 cgggactgtg cgactcgcca cccggcaggt cgatcatgac ctcctcatag gccaggccct
      721 tctccgcgag cgcgatccgc acgcgccatg gcatcagcga aaccgggtgc cagtagagtt
      781 tcatggagcc gctccccgct gacccgggcg ataggtctcg accacattgc cggcgagctg
      841 ggcctcggtg aacagcaccg gcttgaaccg catggcggcg aacagcggga cctggtcgct
      901 gtagtgtggc gcggaggcgt cgagggtcgc cgcgccgaac tggtggatgc tctggctcga
      961 gaggacgccg tcgcgatccc aggtgacgaa catgatgaag gtgtcgccgg ccacggcggc
     1021 cagccgtccg tcgtctgcgg gcttgccgta aaccgcgcgc agcgtgtccg gcccgccgtc
     1081 gatcggcagg tcgacactgc cgcgacggat gcggttgacc tgaccccatt ccggatcgag
     1141 ccgcccgaaa tgcctccgga gcagcgtgat cgtgtcgatc agggcctggc gtgcggtagg
     1201 ggccttgggg tcgtggctgt cgcgggccgt cagtacgatc tgcgccggca ggatggccag
     1261 ggcggcgccg cggctggtga cccttgtcgt gtagtcccac cgggcaagga tggcctgggc
     1321 ggcggcgatg tcggcgtcgc ccttcgggtc gagaccgagc agttcaccga caaaggccgc
     1381 gacgtccgag cggtggctgt aggtcacgtc gaacttgtag gcgcggaact cctccagcgt
     1441 gatctggtcg tcggcggcga aggtctcgac ctcgcgcagc gaccggttgg tcatgttggt
     1501 ctggatgccc atcgtcggcg aaaaggccca gggatcgagg ccgtcaccgg cggccgtggc
     1561 ctggaacggc gtgttattgg cgttgaacac cagcccgccc ttcggtcgcc acacctgcgg
     1621 gacgcggtcg aacggcagat agcccttcca gatcaggtcc gagcggtcgc cgggcagaac
     1681 tcctgaccag tcgacgccct cgcggcggtt ggggaacagg ccgttgtaga cgtagccgat
     1741 gttgcccttc tcgtcggcgt agacgtagtt gatgctcggc agcgcctgca gggccatggc
     1801 ggcgcgccat tcgtcgatac tggccgcctt gtcgagccgc cagtactgca gcggctggcg
     1861 caccgtatcc atcccggcgt agcggatggc gaacaccccg cccttcgtcc gcatgaccgg
     1921 gccatggacc gaccgcagga cctccttgtt gacggtccag ctgaacggtc cccacagccg
     1981 gacgcgcagg cgggcggtcg acttctcgaa tttccgccat ttgccgtcga ggcggtattc
     2041 atcctcgtcg gccggattga tcgtcagccg gtagatatcg acgaggtcgg ggctgttgac
     2101 cgtgttggcc cagcccagat gcgcattgtg gccgtgcagc atgaacggcg agccgggaaa
     2161 gaagccgccc gcgacatgcc agccttcgcc gctctcgacc accgcctcgt accaggccac
     2221 gggcccgctg tagggctggt gcgaattgac cagcagccgg gtcgcgccgt ccgacgaacg
     2281 cgacggggcc acggccaccc cgttggagcc cttgggcagg cc
//
LOCUS       DAORNX010000462         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34762_length_2315_cov_7.693805, whole genome shotgun sequence.
ACCESSION   DAORNX010000462 DAORNX010000000
VERSION     DAORNX010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34762_length_2315_cov_7.693805"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLO65_15230"
     CDS             <1..175
                     /locus_tag="PLO65_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29652.1"
                     /translation="GLFILAGLGSRGFCTAPLLAEHVAALALDAPSPLPAPLASLVDP
                     ERFTARGRRRISS"
     gene            234..644
                     /locus_tag="PLO65_15235"
     CDS             234..644
                     /locus_tag="PLO65_15235"
                     /inference="COORDINATES: protein motif:HMM:NF039797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HRD29653.1"
                     /translation="MKTLTIAGLVAGLALSAGACAMASEEPAAKPTTARACFFSHQIN
                     GWRDSGSPRDNVVYLDVNANDVYKLDMFGPCNGINEALSIGVETRGGGSAICDGLDVT
                     LIVRGPTGPYRCPVSKITKLTPEEAKALSAAKRR"
     gene            791..1261
                     /locus_tag="PLO65_15240"
     CDS             791..1261
                     /locus_tag="PLO65_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HRD29654.1"
                     /translation="MDDGISHQGAPFRPEVFFLGQTEGWGVARGPTGRLLRRCRIQTE
                     GRLDETYRAIHFDETFLWEDGERDEWRWAMSRGLDGRYVAAEALAGAGIEGRFEGPDY
                     ILSFRRALRTDGGARPRFVTRFTQVSPEVALKSVQILHWGLPLGRLTAFHRRLA"
     gene            complement(1287..>2315)
                     /locus_tag="PLO65_15245"
     CDS             complement(1287..>2315)
                     /locus_tag="PLO65_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRD29655.1"
                     /translation="EVFALLAQRARLDAERRHAPAFGEPPEFVALPPEDRDLAAEAMV
                     LQRQQFDARRRSIDAQRGVLQQRIGQLNQQVTGYQRQLESNRTQQRLIGEELEGMRKL
                     AVQGYAPVNRVRALERNEAALAGEDGSYQAQIARSAEAIGETRMQLLSLDRQMIEEVT
                     GQLRDVEVRLDELQPKLAALRQQLARATVRAPEGGKVVGLKVFTVGGVVGPGDVLMEI
                     VPQDRALVIQAMVAPNDADDLRVGQETQIRFTSLHERDLPILKGRLTELSADSFVDEK
                     SGQRFFRAEIAVPPAELERIRKVRGPDTGLQAGLPVEVLVPLRKRTALDYLLEPLVQT
                     FWRSGREH"
BASE COUNT          320 a          839 c          796 g          360 t
ORIGIN      
        1 cggcctgttc atcctcgccg gcctcgggtc gcgcggcttc tgcaccgcgc cgctgctggc
       61 cgagcatgtc gccgccctgg cgctcgacgc gccctccccg ctgcccgccc cgctggcgag
      121 cctggtcgac ccggaaagat tcacggcgcg cgggcggcgg cgcatctcgt cctgatcgtg
      181 ccggcgagct atatacaggc ggggaatggg aaacacctgt cgggagaccc gtcatgaaga
      241 cccttaccat cgccggcctt gtcgcagggc ttgccctgtc tgcgggagcg tgcgccatgg
      301 cctcggaaga gccggccgcc aagccgacca cggcccgcgc ctgcttcttc agccatcaga
      361 tcaatggctg gcgcgacagc ggttcgccgc gcgacaacgt cgtctatctc gatgtcaacg
      421 ccaacgacgt ctacaagctc gacatgttcg ggccgtgtaa cggcatcaac gaggccctgt
      481 cgatcggcgt cgagacgcgc ggcggcggat cggccatctg cgacggcctg gacgtcaccc
      541 tgatcgtccg gggaccgacc ggcccctatc gctgcccggt gtcgaagatc accaagctga
      601 cgcccgagga agccaaggcc ctgtccgccg ccaaacggcg ctaggaccag tcccgggccg
      661 tctgcgacag tcggtcgcaa gccgtgacgg caaccattca tcccccgcga cattccgcat
      721 cgtggccggg atgaatggcg ggcgttaggg tctcgcataa cgccgttcag cagccggcgg
      781 gggtgcgacc gtggacgacg ggatttctca tcagggcgcg ccgttccggc ccgaggtctt
      841 cttcctcggc cagacggagg gctggggcgt cgcgcggggc cccaccggcc gcctgctgcg
      901 ccgttgccgc atccagaccg aaggccggct cgacgagacc tatcgcgcga tccacttcga
      961 cgagaccttc ctctgggagg acggcgaacg cgacgaatgg cgctgggcca tgagccgggg
     1021 ccttgatggt cgctacgtgg ccgccgaggc gctggccggc gccggcatcg agggccgctt
     1081 cgagggtccg gactacatcc tctcgttccg tcgcgcgctg cggaccgacg gcggcgcgcg
     1141 gccgcggttc gtcacccgct tcacccaggt ctcgccggag gtcgccctca agagcgtgca
     1201 gatcctgcac tggggcctgc cgctgggccg gctgaccgcc ttccaccggc gccttgcctg
     1261 aaggcctgcc caggggccgc ctgatcctag tgttcgcggc ccgaccgcca gaaggtctgc
     1321 accaggggtt cgagcaggta gtccagcgcc gtgcgcttgc gcagggggac cagcacttcg
     1381 accggcaggc cggcctgcag gccggtatcg ggcccgcgga ccttgcggat tcgctccagc
     1441 tccgccggcg gcacggcgat ctcggcgcgg aagaaccgct ggccggactt ttcatcgaca
     1501 aagctgtccg ccgacagctc ggtcagccgc cccttgagga tcggcaggtc ccgctcatgc
     1561 agcgaggtga agcggatctg ggtctcctgc ccgaccctca ggtcgtcggc gtcgttgggg
     1621 gcgaccatcg cctggatcac cagcgcccgg tcctggggga cgatctccat cagaacgtcg
     1681 cccggtccga ccacgccgcc gaccgtgaac accttgaggc cgacgacctt gccgccctcg
     1741 ggcgcgcgca cggtcgcccg ggccagctgc tggcgcaggg cggccagctt gggctgcagt
     1801 tcgtcgaggc ggacctcgac gtcgcgtagc tggccggtga cttcctcgat catctggcgg
     1861 tccagcgaca ggagctgcat gcgcgtctcg ccgatcgcct ccgcggaacg ggcgatctgg
     1921 gcctggtagg acccgtcctc gccggccagg gccgcctcgt tgcgctcgag cgcgcgcacc
     1981 cggttgaccg gcgcatagcc ctgaacggcc agcttgcgca tgccctcgag ttcctcgccg
     2041 atcaggcgct gctgcgtgcg gttcgactcg agctggcgct ggtagcctgt aacctgctgg
     2101 ttcagctgac cgatacgctg ctgcagcacg ccgcgctggg cgtcgatcga gcggcgccgg
     2161 gcgtcgaact gctggcgctg cagcaccatc gcctcggcgg ccaggtcgcg gtcctccggc
     2221 ggcagggcga cgaactcggg cggttcgccg aacgccggcg cgtgtcgccg ctcggcgtcc
     2281 agtcgcgccc gctgggcaag cagggcgaac acctc
//
LOCUS       DAORNX010000463         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_34763_length_2315_cov_6.697788, whole genome shotgun sequence.
ACCESSION   DAORNX010000463 DAORNX010000000
VERSION     DAORNX010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34763_length_2315_cov_6.697788"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1329)
                     /locus_tag="PLO65_15250"
     CDS             complement(28..1329)
                     /locus_tag="PLO65_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD29656.1"
                     /translation="MKIAVIGSGVAGLGAAWALEGVHDLVLYEAADRLGGHANTVTVD
                     YDGLAMAVDTGFIVYNEDNYPNLRGLFSALEVETRLTDMSFACAGGGVEWSSNFPRGV
                     FAQKRNLVRPDFLGMLADIRRFYAVARADLLRGDIDDLTLSAWLRLRRFGGGFRDRFL
                     LPMGAAIWSTTEGGIGDFPAESFLRFLANHGLLQFKPPKWRSVAGGSRTYVDRIGQAL
                     GSSVRLRAAVVEVRRTGGEVIVRDSRGGEDRFDQVILACHAPDALAMLAEPDAEERAL
                     LSAIRYAPNRAWLHRDPGLMPSRESAWGSWNYVADAGGQTAPYVTYWMNNLQGLDAAR
                     PLFVSLNAPPPDPALTFGAFDYEHPQFDSAALAAQRRFGRIQGRGGVWYAGAWLGYGF
                     HEDGVTSGVKAALALGGQVPWTFVDHRVAALAEPPALASAA"
     gene            complement(1326..>2315)
                     /locus_tag="PLO65_15255"
     CDS             complement(1326..>2315)
                     /locus_tag="PLO65_15255"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRD29657.1"
                     /translation="FEAEERAAIAEVTPSPTPAELPAPAAWPASERLADWGLQPTNPD
                     WSTGFACWTPGEAGAAAALDQFLDGRLDGYGANRDRPGVDGTSRLSPHLHFGEIGPRQ
                     VWRAAQSAVALSGAREGEADRFLAELGWREFNHHLLFHWPDLPHDNFKPQFDHFVWRE
                     DEPAFRAWSRGRTGYPVVDAGMRELWTTGFMHNRVRMIVASFLVKDLMIDWRRGEAWF
                     WDTLVDADVAQNAANWQWVAGSGADASPFFRIFNPVTQGRTFDPEGVYVRRWLPELAG
                     LPDAVLHAPWTASPLDLRQAGVVLGRTYPHPIVDHARARERALTAWAAVRSAA"
BASE COUNT          367 a          789 c          849 g          310 t
ORIGIN      
        1 cggtctcccc gacataaagg ctggcggtca tgcggccgag gccagggccg gcggctcggc
       61 cagggccgcg acccgatgat cgacgaaggt ccaggggacc tggccgccga gggcgagcgc
      121 cgccttgacg ccggaggtca caccgtcctc gtggaagccg tagcccagcc aggcgccggc
      181 gtaccatacc ccgccacgcc cctggatgcg gccgaaccgg cgctgggccg ccagggcggc
      241 gctgtcgaac tgcgggtgtt catagtcgaa ggcgccgaac gtcagggccg ggtcgggcgg
      301 gggggcgttg aggctgacga acaggggccg ggcggcgtcg agcccctgca ggttgttcat
      361 ccagtaggtc acatagggcg cggtctggcc gccggcgtcg gccacatagt tccagcttcc
      421 ccaggcggat tcacggctcg gcatcagacc ggggtcgcgg tgcagccagg cgcggttcgg
      481 agcatagcgg atggccgaca gcagggcccg ctcctctgca tcgggctccg ccagcatcgc
      541 cagggcgtcc ggcgcatggc aggcgaggat cacctgatcg aagcggtcct cgccgccccg
      601 actgtcgcgg acgatcacct cgccgcccgt gcggcggacc tcgacgaccg cggcgcggag
      661 cctgacgctg gagccgaggg cctggccgat gcggtcgaca taggtgcggc tgccgccggc
      721 cacgctgcgc catttcggtg gcttgaactg cagcaggcca tggttggcca ggaagcgcag
      781 gaagctctcg gccgggaagt cgccgatgcc gccctcggtc gtcgaccaga tggccgcgcc
      841 catcggcagc aggaaccggt cgcggaagcc gccgccgaac cggcgcagcc tgagccaggc
      901 gctcagcgtc aggtcatcga tgtcgccccg cagcaggtcg gccctggcca ccgcgtagaa
      961 ccggcgaata tcggcgagca tgcccagaaa gtcgggccgc acgaggttgc gcttctgggc
     1021 gaacacgccg cgcggaaagt tcgacgacca ttcgaccccg ccgccggcgc aggcgaagct
     1081 catgtcggtc agacgagtct cgacctcaag cgcgctgaac aggccgcgca ggttggggta
     1141 attgtcctcg ttgtagacga tgaaccccgt gtccacggcc attgccaggc cgtcgtagtc
     1201 gacggtgacc gtgttggcgt ggccgcccag gcgatcggcg gcctcgtaga gaaccaggtc
     1261 atgcacgccc tcgagcgccc aggcggcccc cagtccggcg acgcccgatc cgatgacggc
     1321 gatcttcatg cggcgctcct gacggcggcc caggcggtga gggcccgttc gcgggcccgg
     1381 gcgtggtcga cgatgggatg gggataggtg cgaccgagga cgacgccggc ctgtctcaga
     1441 tcaaggggcg aggcggtcca cggcgcgtga agcaccgcgt ccggcagtcc ggcgagttcg
     1501 ggcagccatc gccgcacata gacgccctca ggatcgaagg tgcggccctg ggtcaccggg
     1561 ttgaaaatgc ggaaaaaggg cgaggcgtcg gcgcccgagc cggccaccca ctgccagttg
     1621 gccgcgttct gggcgacgtc agcgtcgacc agcgtgtccc agaaccaggc ctcgccccgc
     1681 cgccagtcga tcatcaggtc cttgaccagg aaggaggcga cgatcattcg cacgcgattg
     1741 tgcatgaatc cggtggtcca cagttcgcgc atgccggcgt cgaccaccgg ataacccgtg
     1801 cggccacgcg accaggcgcg gaaggcgggc tcgtcctcgc gccatacgaa atggtcgaac
     1861 tgcggcttga agttgtcgtg cggcaggtcg ggccagtgga acagcaggtg atggttgaac
     1921 tcgcgccagc ccagctcggc gaggaacctg tcggcctcgc cctcccgcgc cccgcttagc
     1981 gccacggcgg actgcgccgc gcgccacacc tgccggggac cgatctcgcc gaaatgaaga
     2041 tggggcgaca ggcgcgaggt tccgtccacg cccgggcggt cgcggttggc gccatagcca
     2101 tcgaggcgac cgtcgagaaa ctggtcgagg gccgccgcgg cgccggcttc gccaggcgtc
     2161 cagcaggcaa agccggtcga ccagtcggga ttcgtcggct gcaggcccca gtcggccagg
     2221 cgttcgctgg cgggccaggc ggccggcgcg gggagctccg cgggcgtcgg cgaaggtgtg
     2281 acctcggcga tggcggcgcg ctcctcggcc tcgaa
//
LOCUS       DAORNX010000464         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35001_length_2302_cov_9.338674, whole genome shotgun sequence.
ACCESSION   DAORNX010000464 DAORNX010000000
VERSION     DAORNX010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35001_length_2302_cov_9.338674"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLO65_15260"
     CDS             <1..522
                     /locus_tag="PLO65_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29658.1"
                     /translation="RLGEENDGWTVAKYLLEFERGGSSAPGLRGSVARLKAMAANEVS
                     DEGAPLIDDPDFRARLAEAEILVESIEITEHRVMSALAGGKNPGPASSMLKTQSTECT
                     QRLDELAIEIANHYAAVDQPGAREPGSNQAPVGPEHSLVAMARYLNNRAASIYGGSNQ
                     IQRDIMARLVLRL"
     gene            534..932
                     /locus_tag="PLO65_15265"
     CDS             534..932
                     /locus_tag="PLO65_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRD29659.1"
                     /translation="MARAVATIGTTDWATTVVTGRHSLVVDEPPALGGQDAGPAPYDV
                     LLASLGACTAITLRMYATRKGWPLAAAEVDLKLYKDGDRSKIERVLRLTGDLDDTQRA
                     RLADIAERTPVTLTLKAGADIVTTLAQAGA"
     gene            complement(1127..2302)
                     /locus_tag="PLO65_15270"
     CDS             complement(1127..2302)
                     /locus_tag="PLO65_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29660.1"
                     /translation="MEAELKQGGFANNGLKSVDPIVASKAWQKKKAEAGWACLHWPKE
                     YGGRGATPIERVIWGQEEGVYGALSGLFIIGHGMCGPTVMAWADEATKERLLPPLAAG
                     EHIWCQLFSEPAGGSDLAGLRTAAVPATDGTGDWIINGQKIWTSGAQHSDYGIVITRT
                     DPNVPKHKGLTMFWLDMKSPGIEVRPIKQANGQSGFNEVYFTDVRIPDSQRLGAVGQG
                     WSVSLTTLMNERLSIGSGMPTGFPELFDFCMNLRTEDGLAIDDRSVRSRLADYAVKAS
                     GLKYTGMRAISALSKGETPGPENSIGKLVAGATMQDLAMFALDLQGQAGLLSDPSQAA
                     AGARFQQMLLRSPATRVEGGTDEILRNIIAERVLGLPGDIRVDKDVPFKDIPTSGRG"
BASE COUNT          354 a          804 c          791 g          353 t
ORIGIN      
        1 cggctgggcg aggagaacga cggctggacg gtggccaagt acctgctcga gttcgagcgc
       61 ggcggcagtt cggcccctgg cctgcgcggc tctgtcgcgc ggctgaaggc gatggcggcg
      121 aacgaggtgt ccgacgaggg cgccccgctg atcgacgacc cggacttccg cgcgcgtctg
      181 gccgaggccg agatcctcgt cgagtcgatc gagatcaccg agcaccgcgt gatgtcggcg
      241 ctggccggcg gcaagaaccc cggccccgcc tcatcgatgc tcaagaccca gtcgaccgaa
      301 tgcacccaga gactggacga actggcgatc gagatcgcca accactacgc ggccgtcgac
      361 cagcccgggg cgcgcgaacc gggttcgaac caggcgccgg tcggtcccga gcacagcctg
      421 gtcgccatgg cgcgctacct caacaaccgc gcggcctcga tctatggtgg ctccaaccag
      481 atccagcggg acatcatggc caggctggtg ctcaggctct agggaggccg cccatggccc
      541 gcgccgtcgc gaccatcgga accacggact gggcgaccac ggtggtcacg gggcggcaca
      601 gcctggtggt cgacgagccg ccggccctcg gtggccagga cgccggcccg gcgccctatg
      661 acgtgctgct ggccagcctc ggcgcctgca ccgccatcac cctgcgcatg tacgccaccc
      721 gcaagggatg gccgctggcg gcggcggaag tcgatctcaa actgtacaag gacggcgacc
      781 ggtcgaagat cgagcgggtg ctgcgcctga ccggcgatct cgacgacacg cagcgcgccc
      841 ggctggccga catcgccgag cgcaccccgg tgaccctgac cctcaaggcg ggggccgata
      901 tcgtcacgac cctcgcgcag gccggcgcgt gagcgaggtg aagccggcga acatccactg
      961 tcatcccgga cggcccgaag gggcgatccg ggacccagat tggatgccat ccatcccgcg
     1021 tgacgccaaa tctgggtccc ggctcttcgc tgcgctgcgg ccgggatgac aatgggtgcg
     1081 ccgcaaaggt ccagatcggg cccttcgcca gaagcgtagc ctgctcctat ccgcgtccgg
     1141 aggtcggaat gtccttgaac ggcacgtcct tgtccacgcg gatgtcgcca ggcagaccca
     1201 gcacgcgctc ggcgatgatg ttgcgcagga tctcgtcggt gccgccctcg acccgggtag
     1261 ccggcgagcg cagcagcatc tgctggaacc gggccccggc cgccgcctgg gatgggtcgg
     1321 acagcagccc ggcctggcct tgcaggtcga gggcgaacat cgccaggtcc tgcatggtcg
     1381 ccccggcgac cagcttgccg atggagttct ccggcccggg ggtctcgccc ttgctgaggg
     1441 ccgagatggc gcgcatgccc gtgtacttca ggccgctggc cttgacggcg tagtcggcca
     1501 ggcgcgagcg caccgaccgg tcgtcgatcg ccaggccgtc ctcggtgcgc aggttcatgc
     1561 agaagtcgaa cagctccggg aagcccgtcg gcatgcccga accgatcgaa aggcgctcgt
     1621 tcatcagggt ggtcagcgac acgctccagc cctgaccgac cgcgcccagc cgctggctgt
     1681 cgggaatgcg cacgtcggtg aagtagacct cgttgaaacc agactggccg ttggcctgct
     1741 tgatcggccg cacctcgatc ccgggtgact tcatgtcgag ccagaacatc gtcaggccct
     1801 tgtgcttggg cacgttcggg tcggtgcggg tgatgacgat gccgtagtcg ctatgctggg
     1861 cgccgctggt ccagatcttc tggccgttga tgatccagtc gcccgtaccg tccgtggcgg
     1921 gcacggcggc cgtgcgcagc ccggccaggt cggagccgcc ggccggctcg ctgaacagct
     1981 ggcaccagat gtgttcaccg gcggccagcg gcggcaatag ccgttccttg gtcgcctcgt
     2041 cggcccaggc catgaccgtc ggaccgcaca tgccgtggcc gatgatgaac aggccgctga
     2101 gcgcgccgta gacgccctct tcctggcccc agatcacccg ctcgatcggc gtggcgccgc
     2161 ggccgccgta ttccttgggc cagtgcaggc aggcccagcc ggcctccgcc tttttcttct
     2221 gccaggcctt ggaggcgacg atcgggtcga cgcttttgag gccgttgttg gcgaagccgc
     2281 cctgcttcag ttcggcctcc ag
//
LOCUS       DAORNX010000465         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35300_length_2288_cov_6.963726, whole genome shotgun sequence.
ACCESSION   DAORNX010000465 DAORNX010000000
VERSION     DAORNX010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35300_length_2288_cov_6.963726"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLO65_15275"
     CDS             <1..223
                     /locus_tag="PLO65_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRD29661.1"
                     /translation="EAFAHGAVNETAERHPATGQMFIINPLTGRGADNLFSTHPNTDN
                     RIEALLGMTGRPRATGTAVPRTARSGPWG"
     gene            complement(206..598)
                     /locus_tag="PLO65_15280"
     CDS             complement(206..598)
                     /locus_tag="PLO65_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29662.1"
                     /translation="MSDDLNRVARSLTDLMLRAAPGMAVKDAGTRGVHLLARWDNPLK
                     PGQPMWFGGVQTTKTYVSVHLMPVYSHPALAASIPPTLRKRMQGKSCFNFRTEDPALF
                     AELETLIRAAAAVYARPFTLERPSAPRA"
     gene            698..1951
                     /locus_tag="PLO65_15285"
     CDS             698..1951
                     /locus_tag="PLO65_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRD29663.1"
                     /translation="MNRLFTTLIVLAMVAGVAVGWACNQYLSADQTAEAVTWFKVVTD
                     VFLRLIKMIIAPLVFSTLVAGIAHMGDAASVGRVGAKTMFWFITASIFSLLLGLGMVH
                     LLQPGVGLTLPAPEASASAVDASKLTLQEFITHVFPSSIFDAMARNEILQIVVFSIFV
                     GVAVASLDDKAPQILVLVEQLAAIMLKVTGYVMRLAPLAIFAALASTVAKYGIDILAI
                     YAKFIGGFYLSLGVLWAMLALFGLLIVGPRIFRLLGMVRQPILLAFATASSEAAYPRT
                     LEQLQRFGVSRRVASFVLPLGYSFNLDGSMMYCTFATLFIAQIYGVEMTVGQQVTMLL
                     LLMVTSKGMAGVPRASLVVIAATLAYFDLPEAGVALILAVDQILDMGRSATNVVGNSV
                     AAAVVARWEGQLGESEPDPPASAAA"
     gene            2034..>2288
                     /locus_tag="PLO65_15290"
     CDS             2034..>2288
                     /locus_tag="PLO65_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD29664.1"
                     /translation="MSVSVTRDGAITVVSIDRPERRNAVDGPTARALYEAFVAFDADD
                     DASVAVLAGSGGNFCAGADLKAIAGGGGNRVERTGDFGPMG"
BASE COUNT          324 a          805 c          762 g          397 t
ORIGIN      
        1 ggaggccttc gcccatggcg cggtcaacga gacggccgag cgccatccgg cgaccggcca
       61 gatgttcatc atcaatccgc tcacgggccg gggcgccgac aacctgttct ccactcaccc
      121 gaacaccgac aatcgcatcg aggccctgct cggcatgacg ggccggccgc gcgccacggg
      181 gacggcggtc ccgcgcacgg cgcggtcagg cccgtggggc tgacggccgc tcgagggtga
      241 aggggcgcgc atagaccgcc gccgccgcgc ggatcagcgt ctcgagctcg gcgaacagcg
      301 ccggatcctc ggtcctgaag ttgaaacagc tcttgccctg catgcgcttg cgcagcgtcg
      361 gcgggatgct cgccgccagg gccgggtggc tgtagacggg catcaggtga acgctgacgt
      421 aggtcttcgt cgtctggacg ccgccgaacc acatcggctg accgggcttc aagggattgt
      481 cccagcgcgc cagcaggtgc acgccgcgcg tcccggcgtc cttcaccgcc atcccgggcg
      541 cggcccgcag catgagatcg gtcagggacc tggccaccct gttcagatcg tcgctcattg
      601 cgcgcgtctc cccggcctcg tcccaacata gcgcaagcgc atggacgcgg gcgacgatcc
      661 cggtgaaaaa gggcgttccg gtttctgggg actccgcatg aatcgcctgt tcaccaccct
      721 catcgtcctg gccatggtgg cgggcgtcgc cgtcggctgg gcctgcaacc agtatctgag
      781 cgcggaccag acggccgagg ccgtgacctg gttcaaggtg gtcaccgacg tcttcctgcg
      841 gctgatcaag atgatcatcg ccccgctggt cttctccacc ctggtcgccg gcatcgcgca
      901 catgggcgac gcggccagcg tcgggcgggt cggggccaag acgatgttct ggttcatcac
      961 cgcctcgatc ttctcgctgc tgctgggcct tggcatggtc catctgctgc agcccggcgt
     1021 gggcctgacc ctgccggcgc cggaggcctc ggccagcgcc gtcgacgcct ccaagctgac
     1081 gctgcaggag ttcatcaccc acgtcttccc cagctcgatc ttcgacgcca tggcccgcaa
     1141 cgagatcctg cagatcgtgg tgttctcgat cttcgtcggc gtggcggtgg ccagtctcga
     1201 cgacaaggcc ccgcagatcc tggtgctggt cgaacagctg gcggccatca tgctcaaggt
     1261 gaccggctat gtgatgcggc tggcgcccct ggccatcttc gcggccctgg ccagcacagt
     1321 ggccaagtac ggcatcgaca tcctggccat ctacgccaag ttcatcggcg gcttctacct
     1381 gtcgctcggc gtgctgtggg cgatgctggc cctgttcggc ctgctgatcg tcgggccacg
     1441 catcttccgg ctgctgggca tggtccgcca gccgatcctg ctggccttcg ccaccgccag
     1501 ttcggaggcc gcctatccgc gcaccctcga acagctgcag cggttcggcg tcagccggcg
     1561 cgtggcctcg ttcgtgctgc cgctcggcta ctcgttcaat ctggacgggt cgatgatgta
     1621 ttgcaccttc gcaaccctgt tcatcgccca gatctacggc gtcgagatga ccgtcggaca
     1681 gcaggtgacc atgctgctgc tgctgatggt cacgtccaag ggcatggccg gcgtgccgcg
     1741 cgcctcgctg gtggtgatcg ccgccaccct ggcctatttc gacctgcccg aggccggcgt
     1801 cgccctgatc ctcgcggtcg accagatcct cgacatgggc cgctcggcga ccaacgtggt
     1861 gggcaattcg gtcgccgccg cggtcgtggc caggtgggaa ggccagctgg gcgaaagcga
     1921 accggacccc ccggcgtcgg cggcggcctg agggcgtttc gaaaaggact tgtgttccgc
     1981 acacggcgcc cgctcatcct gaccatccga tccgtctgga ggaggttcct ggcatgagcg
     2041 tttccgtgac gcgcgacggc gcgatcaccg tcgtctcgat cgaccggccc gagcggcgca
     2101 acgcggtcga cggcccgacc gcccgggcgc tgtacgaggc gtttgtcgcc ttcgacgccg
     2161 acgatgacgc cagcgtggcc gtgctggccg gatccggcgg caacttctgc gccggcgccg
     2221 acctcaaggc catcgccggc ggcggcggca accgggttga gcggaccggc gacttcggcc
     2281 cgatgggt
//
LOCUS       DAORNX010000466         2284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35378_length_2284_cov_7.408255, whole genome shotgun sequence.
ACCESSION   DAORNX010000466 DAORNX010000000
VERSION     DAORNX010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35378_length_2284_cov_7.408255"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLO65_15295"
     CDS             <1..475
                     /locus_tag="PLO65_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29665.1"
                     /translation="HDAVRRRQEDGRGDREEPLMGPRPPRLKFDGPAKPKDARPVLIR
                     FVCFHTVGRQRTRLGLFQAIKLARDSDQSAAWALEEINELNAWFSDHLPVPEPIEHGT
                     RRGDQHRALSWFKPQAVDHVRRMYELKAALEACGVHVEILTTRTPASSPMKTSTS"
     gene            complement(793..1074)
                     /locus_tag="PLO65_15300"
     CDS             complement(793..1074)
                     /locus_tag="PLO65_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRD29666.1"
                     /translation="MTFKLVKARRVRPLPGYRLDLTFSDGSSGVIDISDFVLSGGQVV
                     QPLVDETFFARVFLEMGVPTWPNGCDIDPTNARMKLEAAGLLKAQGAAA"
     gene            1151..1369
                     /locus_tag="PLO65_15305"
     CDS             1151..1369
                     /locus_tag="PLO65_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29667.1"
                     /translation="MKQDPLSDTTGCGPGNPPSQDLSAPQVENGFTFAILRVEMGRIV
                     VLEIDPDDDSEKDGDDRHSGALAGKSNR"
     gene            1409..1816
                     /locus_tag="PLO65_15310"
     CDS             1409..1816
                     /locus_tag="PLO65_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRD29668.1"
                     /translation="MPTEPKTRPTDVPVADFIAAVEHPGRREDAGVIAALMAEVTGLD
                     PVMWGPSIVGFGSYRGPTGDWPVIAFSPRKANLVLYIMKGFKESGDLLARLGKHRTGG
                     GCLYLNRLDGVDMGVLRTLCAESVAWMREKYPS"
BASE COUNT          396 a          754 c          726 g          408 t
ORIGIN      
        1 ccatgatgct gttcgccgac gccaagaaga tggtcgaggg gatcgtgaag aacctctgat
       61 ggggccgagg ccgccgcgtc tgaagttcga cggccccgcg aagccgaagg atgcgcggcc
      121 ggttctgatc cgcttcgttt gtttccatac ggtcggccga cagcgcaccc gccttgggct
      181 gttccaggcc atcaagctcg cccgcgacag cgaccagtcg gccgcttggg cgctcgagga
      241 gatcaacgag ctgaatgcct ggttcagtga ccatctgccg gtgcccgagc caattgagca
      301 cgggacccgc cggggcgacc agcatcgagc cctctcctgg ttcaagccgc aggcggtcga
      361 ccatgtccgc cggatgtatg agctgaaggc ggcgctggag gcttgcggcg ttcacgtaga
      421 gatactgacc acgcggaccc cggcgtcgtc acctatgaag acgagcacca gctgacggcc
      481 gtgccgcgcg gcgggatgtt ctagggccga ggggttttcg cactgcggtc acaccgcccc
      541 gtcgaacgct tgatggaatg gtcctttcgg agacctgttg aagtcaagga cgggcttcgc
      601 ccgccgcggc gcggcagccc gaagggctgt ccttgagttc aacaggcctc cgaaatcatg
      661 ctccgccaag cgatcgatgg cgaccttcgt tcgtcggctc cagcatcgcg ctgctcgggg
      721 acatgcactt cagtgcgtgt ccccatcagc ttctgagcgg cgagggacac gcactgaagt
      781 gcatgtccct gatcaggccg ccgcgccttg cgccttcagc aggccggccg cttccagctt
      841 catccgcgca ttggtgggat caatgtcgca gccgttgggc caggtcggca cgcccatctc
      901 aaggaacacc ctggcaaaga aggtctcgtc gacaagcggt tggacgacct ggccgcctga
      961 cagcacgaag tcgctgatat caatcacgcc gcttgagccg tctgaaaacg tcagatcgag
     1021 cctgtatccg ggcagcggcc tgacccggcg agcttttacg agcttgaagg tcattcctca
     1081 tccagtcccg ggatcttgag ggtaggctgc atattgtacg cccggcccca ttttcggcca
     1141 atgcggactg atgaagcagg atccattgtc tgacacgacg ggttgcggac cgggtaatcc
     1201 tcccagccag gacctctccg ctccgcaggt cgaaaatggc ttcaccttcg ccatactccg
     1261 cgtggagatg gggcggattg tggtcctgga aattgatccg gatgacgatt ccgaaaaaga
     1321 cggagatgat cggcatagcg gcgcgttggc cgggaagagc aatcgttaga gcgttttccg
     1381 acaaaataca accataaggc gtgtagctgt gcccacggaa cccaagaccc ggccaaccga
     1441 cgtgcccgtc gcggacttca tcgcggccgt tgagcatccg gggcgtcgcg aggacgcggg
     1501 agtcatcgcc gccctcatgg ccgaggtcac gggccttgac cccgtcatgt gggggccgag
     1561 catcgtcggc ttcggcagct acagagggcc gaccggcgac tggccggtca tcgccttctc
     1621 gccgcgcaag gccaacctgg tcctctacat catgaagggg ttcaaggagt ccggcgacct
     1681 gctggcccgg ctcggcaagc acaggaccgg cggcggctgc ctgtacctga accgcctcga
     1741 cggggtcgac atgggcgtac tgcggacgct gtgcgcggaa tcggtcgcct ggatgcgcga
     1801 gaaatatccg tcatgacccc gctccgcatc cagcgcgtgg tcgccgttgt cttcccgctc
     1861 ccgggcggct ggcgcagagc aacggccgcc tgaacagagt ctatccccgc ccccggggtt
     1921 gccttgcggg ccttcgcaga ataaaccgcc cgcctcccgg tcataatctc gcccggttgg
     1981 gccagcatat ccgcggcctt caaacatcca tgaccgtcgg agctggtgcg tcagacctcg
     2041 tcgcaccctg cgtatagctc ggcaacagtc cacgaggccg aaccgtgttc cgtgatttgc
     2101 tttcccgaga gagtcgcttt ccgccgttgc tgggcacggg actcgccgcc gtgctgatgg
     2161 gttcggtggc cgtcgtctcg gcccacgcca gcctgccgtc ccgcgaggaa cgcgccgccc
     2221 gcatggtccc ggccgttgcg ccgccgccgc cggccgcccg gccgcacatc gaacaggccc
     2281 gcta
//
LOCUS       DAORNX010000467         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35554_length_2275_cov_7.863063, whole genome shotgun sequence.
ACCESSION   DAORNX010000467 DAORNX010000000
VERSION     DAORNX010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35554_length_2275_cov_7.863063"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLO65_15315"
     CDS             <1..467
                     /locus_tag="PLO65_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRD29669.1"
                     /translation="GHGAATLDGTTAARRLPGDIGYLNPEGLTAAELEDALADLADTR
                     ALILDLRAHGGGEADAMLLGHLSRRPIPMARIRRNPDGGVLTLWTGRATPAPDGPLYP
                     DNPLFVLIASRSALPAQALAYDLRTCGRAAVINGEDALLLAYRRALALRQVI"
     gene            complement(587..1438)
                     /locus_tag="PLO65_15320"
     CDS             complement(587..1438)
                     /locus_tag="PLO65_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HRD29670.1"
                     /translation="MQESENLIETLALEPIELNLFRGRSPNDGFPRIFGGLVIAQALM
                     AAYETVEDRTCHSLHAYFIRPGDVRIPVLYQVDRARDGGSFTTRRVVAIQNGEQIFNL
                     AASFQVAEEGYEYQSPMPAHPTPESVASEMEQALAENKDMPAAMIEHMKRRPVEVRWP
                     DPQNQFKPVPKPPRKQVWLRAKAPIGDSIKLQQAALAYASDMAFMETALRVHGLTWRT
                     KGMQSASLDHAMWFHRPTNFNDWHLFDQDCPSTSQGRGFIRGEMYTQDGVLAASVAQE
                     CLMRVKA"
     gene            1531..2106
                     /locus_tag="PLO65_15325"
     CDS             1531..2106
                     /locus_tag="PLO65_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29671.1"
                     /translation="MRNNAVALIAGAILACAASDATSASSPAPDNGIACEVAEAYVNE
                     VLSRAPDPVIFSDEPFGGSGERRLPRIGWRKDRPSTALAAAYRNASDASPFAECPTLR
                     AYVERRGAKIGSVAIDQATLVPPGAPRPSTWGVTIEVISMPIVSPSGREAVLENGFGC
                     GPLCGGTAIVHLRKGRDGFWVVTDVAMTGIS"
     gene            2152..>2275
                     /locus_tag="PLO65_15330"
     CDS             2152..>2275
                     /locus_tag="PLO65_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRD29672.1"
                     /translation="MPFTATVLTMFPEAFPGPLGVSLIGTAWREQDLWRLETVDI"
BASE COUNT          348 a          769 c          776 g          382 t
ORIGIN      
        1 ccggtcacgg cgccgcgaca ctggacggga cgaccgccgc gcggcgactg cccggcgaca
       61 tcggctatct gaatcccgaa ggcctgacgg ccgccgagct tgaggacgcc ctggccgatc
      121 tggccgacac gcgcgccctg atcctcgacc tgcgagccca cggtggcggg gaggcggatg
      181 cgatgctgct cggccacctg tcccggcggc cgatcccgat ggcgcggatc cgcaggaacc
      241 ccgacggcgg cgtcctgacc ctgtggaccg gtcgcgcgac gccggcgccc gacggcccgc
      301 tgtatccgga caatcccctg ttcgtgctga tcgcgagccg gtcggcgctg ccggcccagg
      361 cgctggccta cgacctgcgc acctgtggcc gcgcggccgt catcaacggc gaggacgcgc
      421 tgctgttggc ctatcggcgg gcgctggcgc tgcggcaggt gatataggga gtagggctta
      481 gggagtaggg agtagggagt agcgagtagc agcagggttg gcgccgttct cggtagcgcg
      541 aacagatcga actactccct actccccaat acctactccc tatgccctac gccttcaccc
      601 gcatcaggca ttcctgggcc accgaggccg ccagcacgcc gtcctgggtg tacatctcgc
      661 cgcggatgaa gccgcgcccc tgcgaggtcg acgggcagtc ctggtcgaac aggtgccagt
      721 cgttgaagtt ggtcgggcga tggaaccaca tggcgtggtc caggctggcc gactgcatgc
      781 ccttcgtgcg ccaggtcagg ccgtggaccc gcagggcggt ctccatgaag gccatgtccg
      841 aggcgtaggc cagcgccgcc tgctgcagct tgatcgaatc gccgatcggc gccttggcgc
      901 gcagccagac ctgcttgcgc ggcggcttgg gcaccggctt gaactggttc tgcgggtcgg
      961 gccagcgcac ctcgaccggg cggcgtttca tgtgctcgat catcgccgcc ggcatgtcct
     1021 tgttctcggc cagggcctgc tccatctcgc tggccacgga ctcgggcgtc ggatgcgccg
     1081 gcatgggcga ctggtattcg tagccttcct cggcgacctg gaacgaggcc gcgaggttga
     1141 agatctgctc gccgttctgg atcgccacca cccggcgggt ggtgaagctg ccgccgtcgc
     1201 gggcgcggtc gacctggtag agcaccggga tccgcacgtc ccccggccgg atgaaatagg
     1261 cgtgcaggct gtggcaggtc cggtcctcga ccgtctcgta ggcggccatc agcgcctggg
     1321 cgatgaccag cccgccgaag atcctcggga agccgtcatt gggcgaccgc ccccggaaca
     1381 ggttcagctc gatcggctca agggccaggg tctcgatgag gttttcgctc tcttgcatag
     1441 gccgcgctta gacccggccg gccccgcggg caagcaggtt gaccccaaac aggaaaggct
     1501 gctgctatcc acggcggaga gaggtctgac atgcgcaaca atgccgtcgc cctgatagcc
     1561 ggcgcgatac tggcctgcgc cgcttccgac gcgacgagcg cttcgagccc ggcacctgac
     1621 aatggcatcg catgcgaagt cgcggaggcc tacgtcaacg aagtcctctc cagagctccc
     1681 gaccccgtga tcttctccga tgagcccttc ggcgggtcgg gcgaacggag gctgccgagg
     1741 attgggtggc ggaaggatcg tccctccacg gcgctagccg ccgcctatcg caatgcatcg
     1801 gacgccagtc catttgcgga atgtccgaca cttcgagcct acgtcgaaag gcgcggcgcg
     1861 aagataggaa gcgttgccat cgaccaggcc acgctggttc cgcccggagc cccgcgcccg
     1921 tcgacttggg gggtcaccat cgaggtcata tccatgccga tcgtttcgcc gagcggtcgc
     1981 gaagctgttc tggagaacgg ctttggctgc ggtccgcttt gcggcggcac ggccattgtt
     2041 catttgcgca aggggcggga cggcttctgg gtcgttacgg acgtggccat gacgggcatt
     2101 tcctgagtcc agcgatggcg aagacggccc gccggcggta agaccccgcc catgccgttc
     2161 accgccactg tcctgaccat gttccccgag gccttccccg gcccgctcgg cgtgtcgctg
     2221 atcggcacgg cctggcgcga gcaggacctg tggcgccttg aaacagtgga cattc
//
LOCUS       DAORNX010000468         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35583_length_2274_cov_5.603876, whole genome shotgun sequence.
ACCESSION   DAORNX010000468 DAORNX010000000
VERSION     DAORNX010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35583_length_2274_cov_5.603876"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="PLO65_15335"
     CDS             <1..1383
                     /locus_tag="PLO65_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29673.1"
                     /translation="ARPQHEDPERARRRAKPDVRPARLRRQPVEQPGQRHEPAKARCN
                     VGRDEGRTDPCPAAARAGDLARAETALGEALTLYPDVPGSYIRLARVQAAAGRSEAAL
                     ANVATYASMGLRLDISRDPALKGLSDLPGWAEVAATFEKNGSPSGDADEIASVIGHPE
                     FIGEGLAHDGKGWLLSTVSGRTIVRLADGGATTPFLKGDADTGAIFGMAVDRQRGLLW
                     AAEAWGDGVPEGTGASKTGLLKVSLVDGRVLARVFLPDDGGAHQFGDVLADPDGTVYA
                     TDSAGGGLWRLRAGEVQLEQVVAPGQYASPQGMAFCAEGKALLVADYSTGLHRVDLAT
                     GVTTPLGGLRAGMAGTDGLLAVPEVDFGLRSASPLPLGVIVTQNGVSPQRIMLLRISP
                     DCDKVEGAHLIAANLPGMEDVSLATKHDGYVVFVGRARWEARGQDGKLTRPDPGPIRV
                     FQASVPGMTF"
     gene            1452..>2274
                     /locus_tag="PLO65_15340"
     CDS             1452..>2274
                     /locus_tag="PLO65_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRD29674.1"
                     /translation="MIDLILDQHRRDLGGFEVGRVLPQAARRMVGPFIFFDRMGPADF
                     QPGFPRSVDVRPHPHIGLSTLSYLFDGEMTHRDSVGSDIVIRPGEVNWMTAGSGITHS
                     ERFEGLREHGGRMDGIQTWLALPAEAEETAPAFSHHAGDADLPFYEGGGMSARLVAGS
                     AFGARASVPVFSPLFYVHWELQPGVTAGLPAEYPERAAYVARGSVEIDGQTIDEGRMV
                     LFAPGQTVPVKALTAASVILVGGEPVGERFIEWNFVSSSKDRIEQAKADWRAGRMK"
BASE COUNT          323 a          801 c          819 g          331 t
ORIGIN      
        1 gcgcggccac agcacgagga ccccgagcgc gcccgccgcc gcgccaagcc ggatgttcgg
       61 cccgcccgcc ttcgtcgcca gccggtcgag caacccggcc agcgccatga acccgccaaa
      121 gcccgctgca atgtagggcg cgacgagggc cgcaccgatc catgcccagc cgccgccagg
      181 gccggcgatc tggccagggc cgagaccgcg ctgggcgaag cgctgacgct ctatcccgac
      241 gttcccggct cctacatccg gctggcccgc gtccaggcgg cggcgggcag gtccgaggcg
      301 gcgctggcca atgtcgcgac ctacgccagc atgggcctgc gcctcgacat cagccgcgac
      361 ccggcgctca aggggctgtc ggacctcccc ggctgggcgg aggtcgcggc gacgttcgag
      421 aagaacgggt cgccatcggg cgatgcggac gagatcgctt cggtgatcgg tcacccggag
      481 ttcatcggcg aaggtctggc ccacgacggc aagggctggt tgctgagcac cgtttccggc
      541 cgcaccatcg tccgcctggc cgatggcggc gccacgacgc cgttcctcaa gggcgacgcc
      601 gacacgggcg cgatcttcgg catggccgtc gaccgccagc gcggcctgct ctgggccgcc
      661 gaagcctggg gcgacggcgt gccggaagga acgggcgcct cgaagaccgg cctgctgaag
      721 gtgtcgctcg tcgacggccg cgtgcttgcg cgcgtcttcc tgcccgacga tggcggcgcg
      781 catcagtttg gcgatgtgct ggctgacccg gacggcacgg tctacgccac cgactcggcc
      841 ggcggcggcc tctggcggct gagggcgggg gaggtgcagc tcgaacaggt ggtcgcgccg
      901 gggcaatacg cgtcgccgca gggcatggcc ttctgcgcgg agggcaaggc cttgctggtc
      961 gccgactatt cgaccggtct gcaccgcgtc gatctcgcga ccggcgtcac gacgccgctc
     1021 ggcggcctgc gcgccggaat ggccggaacc gacggtctgc tcgccgtccc ggaggtcgac
     1081 ttcggcctgc gcagcgcctc gccactgcca ctcggcgtga tcgtcaccca gaacggcgtc
     1141 tcgccgcaga ggatcatgct gctgcggatc agcccggact gcgacaaggt cgaaggcgct
     1201 catctgatcg ccgccaacct gcccggcatg gaggacgtga gcctggcgac gaaacacgac
     1261 ggctatgtcg ttttcgtcgg tcgcgcgcga tgggaggccc ggggccagga cggaaagctg
     1321 acgcgtccgg atcccggtcc gattcgcgtg ttccaggcct ccgtccccgg gatgaccttc
     1381 tagcgacgca ggcccttccg ctgcccggcg ggagaggtca tccttggcgg ccctgcgtat
     1441 ggagccccgc catgatcgac ctgatccttg accagcaccg ccgcgacctg ggcgggttcg
     1501 aggtggggcg ggtgctgccg caggcggcgc gacggatggt cgggccgttc atcttcttcg
     1561 accgcatggg ccccgccgac ttccagccgg gattcccgcg cagcgtcgat gtgcggccgc
     1621 acccgcatat cggcctgtcg accctcagct acctgttcga cggcgagatg acccaccgcg
     1681 acagcgtcgg ctcggacatc gtgatcaggc cgggcgaggt caactggatg accgccggca
     1741 gcggcatcac ccactcagaa cggttcgagg gcctgcgcga gcacggcggc cggatggacg
     1801 gcatccagac ctggctggcc ctgccggccg aggccgagga gaccgccccg gccttctcgc
     1861 atcatgccgg cgacgcggac ctgccgttct acgagggcgg gggcatgtcc gcgcggctgg
     1921 tggccggttc ggcgttcggc gccagggcca gcgtgccggt gttttcgccg ctattctatg
     1981 tgcactggga gctgcagccg ggcgtcacgg cgggcctgcc ggccgagtat ccggagcgtg
     2041 cggcctatgt cgccaggggc agcgtcgaga tcgacggcca gaccatcgac gagggccgga
     2101 tggttctctt cgcgcccggc cagaccgtgc cggtcaaggc tttgacagcc gccagcgtga
     2161 tcctggtcgg cggcgagccg gtcggcgagc ggttcatcga atggaacttc gtctcctcct
     2221 cgaaagaccg catcgagcag gccaaggccg actggcgggc cggccggatg aagc
//
LOCUS       DAORNX010000469         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35644_length_2271_cov_5.678700, whole genome shotgun sequence.
ACCESSION   DAORNX010000469 DAORNX010000000
VERSION     DAORNX010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35644_length_2271_cov_5.678700"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLO65_15345"
     CDS             <1..510
                     /locus_tag="PLO65_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HRD29675.1"
                     /translation="VSLSQRLLFFDARDAAQVAAMRAEGENARYLQGVTLPPALRLSG
                     SATKIGARESDGVLGDACEALMAALYLDGGLEVARTFFNDAWQEEFDNPAAGRDRDPK
                     TQLQEWAQARGLPLPGYSVVRRTGPDHAPTFTVAVTIDGFDAERGEGRSRQEAEKAAA
                     LAMLVRREA"
     gene            607..1554
                     /gene="era"
                     /locus_tag="PLO65_15350"
     CDS             607..1554
                     /gene="era"
                     /locus_tag="PLO65_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRD29676.1"
                     /translation="MSSSSPTRAGFAAIIGAPNAGKSTLTNRLVGAKVSIVTRKVQTT
                     RFPVRGVAMAGQSQIVLVDTPGIFTPRRRLDRAMVRSAWSGAEDADAIVHLVDVQAQL
                     AAGEGKASGADKKAVQDVATIIEGLKGAGRQAILALNKIDGVKRDRMLAVAQALFDTG
                     VYSEVFMISAATGAGVEDLRDRLAALMPEGPWLYPEDQTADLPVRLLAAEITREKLYL
                     RVHEELPYAATVETTAFEERRDGSVRIEQTIFVEREGQRAIIIGENGQTLKWIGSASR
                     EELSDLLDRKVHLFLHVKVRENWADERDIYQGMGLEFDV"
     gene            1727..2218
                     /locus_tag="PLO65_15355"
     CDS             1727..2218
                     /locus_tag="PLO65_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD29677.1"
                     /translation="MRKFVLTLAFLMAVTVSPAHAATPEQDIAATLDALHAAAAKADG
                     AAYFALYTPDATYLGTDAGERWSLAEFKAYAMPYFSKGQGWTYVPRERHITVAPIDCR
                     CVAWFDELLDSASYGTSRGTGALVLTADGWKVSQYALTFPIPNDLAKDMTTTIKAFEA
                     KPK"
BASE COUNT          372 a          804 c          761 g          334 t
ORIGIN      
        1 gtatcgctgt cgcagcggct gctcttcttc gacgcgcgcg acgcggcgca ggtggctgcc
       61 atgcgggccg agggtgaaaa cgcccgatac ctccagggtg tgaccctgcc gccggccctg
      121 cggctctcgg gctcggcgac aaagatcggc gcccgcgagt cggacggggt gctcggcgac
      181 gcctgcgagg cgctgatggc ggccctctac ctcgacggcg gcctcgaggt cgcgcggacc
      241 ttcttcaacg acgcctggca ggaagagttc gacaatcccg ccgccggtcg ggatcgcgac
      301 cccaagaccc agttgcagga gtgggcccag gcccgtggcc tgccgttgcc cggctacagc
      361 gtcgtgcggc gcaccggccc cgaccacgcg ccgaccttca ccgtcgcggt gacgatcgac
      421 ggcttcgacg ccgagcgcgg ggagggcagg tcccgccagg aggccgaaaa ggccgccgcc
      481 ctggccatgc ttgtccggcg ggaggcgtag agaagggggc gcgtgccggg acggtgcttg
      541 tccccttgcg ttgcagtccc cgggatacat gtcccccagc gaccgcctgt gctacaggcc
      601 gccgcaatga gctcttcatc ccccacccgc gcgggtttcg ccgcgatcat cggcgccccg
      661 aacgccggca agtcgaccct gaccaaccgc ctcgtcggag ccaaggtctc catcgtcacc
      721 cgcaaggtcc agaccacgcg ttttccggtg cgcggcgtgg ccatggccgg ccaatcacag
      781 atcgtgctgg tcgacacacc cggcatcttc accccccgcc gcaggctcga ccgggccatg
      841 gtccgctccg cctggtccgg ggccgaggac gccgacgcca tcgtccatct ggttgacgtc
      901 caggcccagc tggccgcggg cgagggcaag gcgtccggcg ccgacaagaa ggcggtgcag
      961 gacgtcgcga ccatcatcga gggcctcaag ggcgccggac gacaggccat cctggccctg
     1021 aacaagatcg acggggtcaa gcgcgaccgc atgttggccg tggcccaggc gctgttcgac
     1081 acgggcgtct attcggaggt gttcatgatc tcggcggcca ccggcgccgg ggtcgaggac
     1141 ctgcgcgatc gcctggcggc cctgatgccg gagggcccct ggctctatcc ggaggaccag
     1201 accgccgacc tgccggtgcg gctgctggcc gccgagatca cccgcgagaa gctctacctg
     1261 cgcgtccacg aggaactgcc ctacgcggcg accgtcgaga ccaccgcctt cgaggagcgc
     1321 cgcgacggct ccgtgcgcat cgaacagacc atcttcgtcg agcgcgaggg ccagcgggcg
     1381 atcatcattg gcgagaacgg ccagaccctg aagtggatcg gctccgcctc gcgcgaggag
     1441 ctcagcgacc tgctcgaccg caaggtccac ctgttcctgc acgtgaaggt ccgcgagaac
     1501 tgggccgacg agcgggacat ctaccagggc atgggcctgg agttcgacgt ctgatccgga
     1561 ctgaacggcg gccccccaaa ttccgttcat cccggcgaag gccgggaccc aggctttttc
     1621 agagcatccg ctcccggttc aggtaaagct cggtcttgcc ggacaggtga gcggctgatt
     1681 caaaaacacc tgggtcccgg ccttcgccgg gatgagcgga ggtaatatgc gcaagtttgt
     1741 cctgacgctg gcgttcctga tggccgtcac ggtgtcaccc gcccatgccg ccacccccga
     1801 acaggacatc gccgcgaccc tggacgccct gcatgcggcg gcggccaagg cggacggggc
     1861 ggcctacttc gccctctaca cgcccgacgc cacctacctc ggcaccgacg ccggcgaacg
     1921 ttggagcctg gccgagttca aggcctacgc catgccctat ttctccaagg gccagggctg
     1981 gacctatgtc ccgcgcgagc ggcacatcac cgtcgcgccg atcgactgcc gctgcgtggc
     2041 ctggttcgac gaactgctcg acagcgccag ctacggcacg agccggggga cgggcgccct
     2101 tgtcctgacg gccgatggct ggaaggtcag ccagtatgcg ctgacctttc cgatccccaa
     2161 cgatctggcg aaggacatga ccaccacgat caaggcgttc gaggcgaagc cgaaataatg
     2221 gaatgggaag acgacgcctt cgtgctgtcg gcgcgcgcgc acggggagac c
//
LOCUS       DAORNX010000470         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_35747_length_2265_cov_7.700452, whole genome shotgun sequence.
ACCESSION   DAORNX010000470 DAORNX010000000
VERSION     DAORNX010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35747_length_2265_cov_7.700452"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLO65_15360"
     CDS             <1..185
                     /locus_tag="PLO65_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29678.1"
                     /translation="PAENPEDKIVCKRVDVSGTRIQLPKKCMSRRDWDRLAGRGKEDI
                     ESAIRASLTRNGVPGG"
     gene            complement(193..1389)
                     /locus_tag="PLO65_15365"
     CDS             complement(193..1389)
                     /locus_tag="PLO65_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD29679.1"
                     /translation="MRRVAIVSPIRTAVGKFQGGLASMTAGDLGAVILRALVERTKLD
                     PERVDDVIFAQGYGNGEAPCIARWSLLAADFPISVPGYQLDRRCGSGLQAIIDAAMMI
                     QTGAADVVIAGGVESMSNTEYYSLDMRSGARAGSVTMHDRLSRGRVMSQPISRFGVIS
                     GMIETADNLARDYNITREQSDEYAVMSHQRATKAWAEGKFDDELVKVAVPQKKGDPVI
                     FEKDEGFRPDATMESLGKLRAIEKDGVVTAGNASQQNDAAAACLVVAEDRLDELGLEP
                     MGWFHSWAAAGCDPSRMGIGPVPAVERLFRRNGLGWKDIDLVELNEAFAPQVLAVLKG
                     WGWDDRDRLNVNGSGISLGHPIGATGGRILANLLRELHRRDGRYGLETMCIGGGQGIA
                     AVFERA"
     gene            complement(1499..>2265)
                     /locus_tag="PLO65_15370"
     CDS             complement(1499..>2265)
                     /locus_tag="PLO65_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29680.1"
                     /translation="PAQQTANEIKAAGGEAVANSESVTSMAAVQGMIQQAMDTFGGLH
                     AIINPAGILRDGMFHKMTDEDWDKVIEVHLRGAYNVSRASVEHFRNQQEGSYVHFTST
                     SGLIGNIGQANYAAAKLGVMGLSRILAMEGAIKNVRSNIIAPFAWTRMIATIPVKDEA
                     SAQRVERMKNAMRADQVAQLAVALASPEAKDVSGQIFAVRGNEVILFDQPRPIRNMAD
                     TTGWTPRKLLDVALPAMKNSFFDTGASASVFPYDPI"
BASE COUNT          313 a          778 c          791 g          383 t
ORIGIN      
        1 cgccggccga gaatcccgag gacaagatcg tctgcaagcg cgtggatgtc agcggtacgc
       61 gcatccagct gcccaagaag tgcatgtccc gcagggactg ggaccggctg gccgggcgcg
      121 gcaaggagga catcgagtcc gccatccgcg ccagcctgac ccgtaacggc gttcccggcg
      181 gctgacgcgg actcaggcgc gttcgaacac cgccgcgatg ccctggccgc cgccaatgca
      241 catcgtctcc aggccatagc gcccgtcgcg gcgatgcagt tcgcgcagca ggttggccag
      301 gatccggccg cccgtcgcgc cgatcgggtg gccaagcgag atgccgctgc cgttgacgtt
      361 cagccggtcg cggtcgtccc agccccagcc cttgagcacc gccagcacct gcggcgcgaa
      421 agcctcgttc agctccacca ggtcgatgtc cttccagccg aggccgttgc gccggaacag
      481 ccgctcgacg gcgggcaccg ggccgatgcc catgcgcgac ggatcgcagc cggcggcggc
      541 ccagctgtgg aaccagccca tcggctcgag accgagctcg tcgagcctgt cctcggccac
      601 caccaggcag gcggcggcgg cgtcattctg ctggctggcg ttgccggcgg tgaccacgcc
      661 gtccttctcg atcgcgcgca gcttgcccag cgactccatc gtcgcgtccg gccggaagcc
      721 ctcgtccttc tcgaagatga ccgggtcgcc cttcttctgc ggcacggcga ccttgaccag
      781 ttcgtcgtcg aacttgccct cggcccaggc cttcgtggcc cgctggtggc tcatgaccgc
      841 gtattcgtcc gactgctcgc gggtgatgtt gtagtcgcgg gccaggttgt cggccgtctc
      901 gatcatgccg ctgatcacgc cgaaacggct gatcggctgg ctcatcaccc ggccgcggct
      961 gaggcggtcg tgcatcgtca ccgagccggc ccgggcgccg ctgcgcatgt cgaggctgta
     1021 gtactcggtg ttgctcatgc tctcgacgcc gccggcgatg accacgtcgg ccgcgcccgt
     1081 ctggatcatc atcgccgcgt cgatgatcgc ctgcaggccc gagccgcagc gccgatcgag
     1141 ctggtagccc ggcacgctga tcgggaagtc ggccgccagc agcgaccagc gggcgatgca
     1201 cggcgcctcg ccgttgccgt agccctgggc gaagatcaca tcgtcgaccc gttcgggatc
     1261 aagcttcgtc cgctcgacca gcgcccgcag gatgaccgcg ccgagatcgc cggcggtcat
     1321 gctggcgaga ccgccctgga acttgccgac cgcggtgcgg atggggctga cgatggcgac
     1381 gcggcgcatg ctgtctctcc gattgtcccc gcgaacgcgg gggtccaggc gacgatggtt
     1441 gcttggggct ggtcttggct tgggtccccg cctgcgcggg gacactcgga cttcggggct
     1501 agatcgggtc gtaggggaac accgaggccg aggcgccggt gtcgaagaag ctgttcttca
     1561 tcgccggcag ggccacgtcg agcagcttgc gcggggtcca gccggtggtg tcggccatgt
     1621 tgcggatcgg ccggggctgg tcgaacagga tcacctcgtt gccgcgcacg gcgaagatct
     1681 ggccgctgac gtccttcgcc tcgggggagg cgagggcgac ggccagctgg gccacctggt
     1741 cggcgcgcat ggcgttcttc atccgctcga cgcgctgggc cgacgcctcg tccttgaccg
     1801 ggatggtggc gatcatgcgg gtccaggcga acggggcgat gatgttggag cggacgttct
     1861 tgatcgcccc ctccatggcg aggatgcgcg acagccccat cacgccgagc ttggccgcgg
     1921 cgtagttggc ctggccgatg ttgccgatca ggccgctggt cgaggtgaag tggacgtagg
     1981 agccttcctg ctggttgcgg aagtgctcca ccgaggcgcg cgagacattg taggcgccgc
     2041 gcagatgcac ctcgatgacc ttgtcccagt cctcgtcggt catcttgtgg aacatgccgt
     2101 cgcgcaggat gccggccgga ttgatgatcg cgtgcaggcc gccgaaggtg tccatggcct
     2161 gctggatcat gccctggacc gcggccatcg acgtgacgct ctcggagttg gccaccgcct
     2221 cgccgccggc cgccttgatc tcgttggccg tctgctgcgc cgggc
//
LOCUS       DAORNX010000471         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36045_length_2251_cov_5.307832, whole genome shotgun sequence.
ACCESSION   DAORNX010000471 DAORNX010000000
VERSION     DAORNX010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36045_length_2251_cov_5.307832"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLO65_15375"
     CDS             complement(<1..290)
                     /locus_tag="PLO65_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29681.1"
                     /translation="MNRRLFLIAASAAATGCAPMVQMAGAPDASFTGARLEDDTFVSF
                     DGARMGLRRWLPEGEPAVVVAALHGMNDYSNAFHFAAPVWAGRGIATYAYDQR"
     gene            complement(333..590)
                     /locus_tag="PLO65_15380"
     CDS             complement(333..590)
                     /locus_tag="PLO65_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HRD29682.1"
                     /translation="MTSPIEDAILDLLRALPPGKSIDPSEVAKLIQPERWQRVLGQVR
                     STAVGMARAGTVVITRHGKPADPDSFRGVYRLRLPQDPSPE"
     gene            668..1120
                     /locus_tag="PLO65_15385"
     CDS             668..1120
                     /locus_tag="PLO65_15385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD29683.1"
                     /translation="MDGGNEVSSGPRLISEGPWAGWRTWDGDPFESQSGPYYFRVEAD
                     GTTRCAFRAERRHMNGGGFMHGGCMLTFADFALFSLANDQLKDTHAVTVSLNGEFVGP
                     AHEGDLVEATGEVIRAGKSMIFIRGLISTGGNPMMSFSGVIKKVARRL"
     gene            1122..1625
                     /locus_tag="PLO65_15390"
     CDS             1122..1625
                     /locus_tag="PLO65_15390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD29684.1"
                     /translation="MALVDATPEDIPAIVAIERNPAYALFIGSFPAEEHAARMASDND
                     RYLVWREDGQVLAFAILSKLAEPHGVVLLKRLGAVQTDTGLGRRMMPALVDLVFEQHA
                     ANRFELDVAVLNPRATHIYRREGFVHEGTVREVYRHREGEYHSSHLFAMLRREWEALP
                     RRRMTQI"
     assembly_gap    1877..1886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1887..>2251)
                     /locus_tag="PLO65_15395"
     CDS             complement(<1887..>2251)
                     /locus_tag="PLO65_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD29685.1"
                     /translation="LVLAPALALAQSLTVTTADGKVTTFTPEALAALPRAEARLGGKS
                     VYEGVSLSAVLREAGVPQGPRLHGKPMQALVVVTGQDGYRAVLSIAETDPSFRAAPII
                     LADRLKSGPLAADEGPLRL"
BASE COUNT          363 a          771 c          777 g          330 t
ORIGIN      
        1 cgctggtcgt aggcgtaggt ggcgatgccc cgtccggccc agaccggggc ggcgaagtga
       61 aaggcgttgg aatagtcgtt catcccgtgc agggccgcga ccaccacggc gggctcgccc
      121 tccggcagcc agcgcctgag acccatgcgg gccccgtcga aactgacgaa ggtgtcgtcc
      181 tcgagccggg cccccgtgaa gctcgcgtcg ggcgcgccgg ccatctgcac catcggcgcg
      241 cagccggtcg cagcggcgct cgcggcgatc aggaacaggc gacggttcat gccgccagtg
      301 tgggcgaagg gctgtggcga agaaagggcg ccctactccg gcgacggatc ctgcggcagt
      361 cgcaaccggt agacgccgcg gaagctgtcc gggtcggccg gcttgccatg tcgggtgatg
      421 accaccgtgc ccgcccgcgc catgcccacg gccgtcgacc gcacctggcc cagcacccgc
      481 tgccagcgct cgggctggat aagcttggcg acctcggacg gatcgatgct cttgcccggc
      541 ggcagggccc tgagcaggtc gagaatggcg tcttcaatcg gcgaggtcat ggccaaaccc
      601 gtgtcacgcc ctgttcgcaa cgggccaaga cgccgctatg aacggcgcgc gaaaggaaga
      661 caagcacatg gacggcggca acgaggtctc cagcggaccg cgactgattt cggaaggccc
      721 ctgggccgga tggcgcacct gggacggcga tccgttcgag agccagtcgg gtccgtacta
      781 tttccgcgtc gaggcggacg ggaccacacg ctgcgcgttc cgggccgagc ggcggcacat
      841 gaacggcggc ggcttcatgc acggcggctg catgctgacg ttcgccgact tcgccctgtt
      901 cagtctcgcc aacgaccagc tgaaggacac ccatgcggtg accgtcagcc tcaacggcga
      961 gttcgtcggg cccgcgcacg agggcgacct ggtcgaggcg accggcgagg tgatcagggc
     1021 cggcaagtcg atgatcttca tccgcggcct gatctcgacc ggcggcaacc cgatgatgag
     1081 cttctccggc gtgatcaaga aagtcgcgag aaggctctga catggccctc gtcgacgcga
     1141 cgccggagga cattcccgcg atcgtggcga tcgagcggaa cccggcctac gccctgttca
     1201 tcggcagctt ccctgccgag gagcacgccg cccgcatggc ttcagacaac gaccgctatc
     1261 tggtgtggcg ggaggacgga caggtgctgg ccttcgccat cctgtcgaag ctggccgaac
     1321 cccacggcgt ggtcctgctc aagcgtctgg gcgccgtcca gaccgatacc ggcctcggcc
     1381 ggcggatgat gcccgccctg gtcgacctgg tgttcgagca gcacgccgcc aaccgcttcg
     1441 agctcgacgt ggcggtgctc aatccgcgag ccacccacat ctaccgacgc gaagggttcg
     1501 tccacgaggg caccgttcgt gaggtctatc gccaccggga gggcgagtac cattcgtccc
     1561 acctgttcgc gatgctgcgg cgggagtggg aagccctgcc ccgccgccgg atgacgcaga
     1621 tttaattcac gtgacacaag cttaactggg cttgggatcg ggccccggcg acaccatctc
     1681 ccgcatgaga gggatgatga tgaagaacct tgttgttctc gcgatttcgg cggccagcgc
     1741 gctcggcgcg atctggatgc tgatcgccgt gccgaccgtg cacagcctgg tctgacgcct
     1801 tgcgcgcctc agggcgccgc cgccaccgtc acagacacca cctgtctcac cccgcgatcg
     1861 ggccgcgcgt cggcgtnnnn nnnnnnccag ccgcagcggg ccttcgtcgg ccgccagcgg
     1921 gccggacttg agccggtcgg cgaggatgat tggcgcggcg cggaaactgg ggtcagtctc
     1981 ggcgatcgac aggaccgcgc gatagccgtc ctgaccggtc accacgacaa gggcctgcat
     2041 cggcttgccg tgcagacgcg gcccctgcgg gacccccgcc tcgcgcagca cggccgacag
     2101 gctgacgccc tcatagacag acttgccgcc cagccgggcc tcggcccggg gcagggccgc
     2161 cagggcctcg ggggtgaagg tggtgacctt gccgtcggcc gtcgtcacgg tcagcgactg
     2221 cgccagggcc agggcggggg ccaggaccag c
//
LOCUS       DAORNX010000472         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36276_length_2239_cov_8.172161, whole genome shotgun sequence.
ACCESSION   DAORNX010000472 DAORNX010000000
VERSION     DAORNX010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36276_length_2239_cov_8.172161"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLO65_15400"
     CDS             <1..1292
                     /locus_tag="PLO65_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD29686.1"
                     /translation="ENTGFYAQAGFLSFYYNYPRPMASAVRGYSDEATIEEVRLVSKA
                     GGDFDYVVGLYFNDDTLVATQESYLRGFKRWWDLWSGFPSAVSGDQDFAYRRHEDFTE
                     KAVYGELTWHASDSLALTGGVRWFDNEAVNTTFIDVPLYTSLSQPVTARFTSSESKAL
                     FKGNLSWTFAENGLFYATVSQGYRRGGSNAVPLTGNFAEDPRWQIYKSDSVVNYEAGV
                     KGRMGSFVYDAAVFYVDWDDIQLNTATPNWGFYVVQNGGKARTYGLETSIDGRPTDAL
                     HYSLGYTYTNARLVDDVVSPLGAVLGTDGSHLPGTPEHAITLAADYTWSLAGDVTLVA
                     RGNGYYQSETRNAIGGPKYNTELAGFTIWNGSATLSRGPWAATFFVKNIFNEEGVTGE
                     FTEAYMGTRPAVGYYGNGSKEFISLPRTLGLSIDFSF"
     gene            1300..>2239
                     /locus_tag="PLO65_15405"
     CDS             1300..>2239
                     /locus_tag="PLO65_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD29687.1"
                     /translation="MASLATRDDVMGELGGVAPLIRDGRLGDALARLASLETRAHDDA
                     RLLQHLGEHFTHCGRSADAARCYRRAAALTPNDPRLLYNLATALIALGQTAEAEALLD
                     QVILLDPDDHDAWQNRSTLRRQTQQSNHTAALLQATRQRLTGPGEVALSYALAKELED
                     LGEHELSFAWLRRGADRRRSLLSYQVEADVAAMAEIARVFDAALFSSPVPGCDEPGPV
                     FVLGLPRSGTTLVDRIISSHSDVESLGEINDFAYALMRVAGPSAAKGDLIRRSARLDP
                     AALGRAYLDSVAGRGRSAPLFIDKTPVNFLYIGLIAR"
BASE COUNT          350 a          809 c          733 g          347 t
ORIGIN      
        1 gcgagaacac cggcttctac gcccaggccg gcttcctgag cttctactac aactatcccc
       61 ggccgatggc ctccgccgtg cgcggctatt ccgacgaggc gaccatcgag gaggtccggc
      121 tggtctccaa ggccggcggg gacttcgact atgtggtcgg gctctacttc aatgacgaca
      181 ccctggtcgc gacgcaggaa agctatctgc gcggcttcaa gcgctggtgg gacctgtgga
      241 gcggcttccc gtcggcggtc tccggcgacc aggacttcgc ctaccgccgc cacgaggact
      301 tcaccgagaa ggcggtctat ggcgagctga cctggcatgc cagcgacagc ctggccctga
      361 ccggcggcgt ccgctggttc gacaacgagg cggtcaacac gaccttcatc gacgtgccgc
      421 tgtacacgtc gctgagccag ccggtgaccg cgcgcttcac ctcctcggag agcaaggcgc
      481 tgttcaaggg caacctgtcc tggacgtttg ccgagaacgg cctgttctac gccaccgtgt
      541 cccagggcta tcgccgcggc ggctcgaacg ccgtgccgct gaccggcaac ttcgccgagg
      601 acccccgctg gcagatctac aagtccgact cggtggtcaa ctacgaagcc ggcgtgaagg
      661 ggcgcatggg gtcgttcgtc tatgacgcgg cggtcttcta tgtcgactgg gacgacatcc
      721 agctgaacac ggccacgccg aactggggct tctacgtcgt gcagaacggc ggcaaggcgc
      781 ggacctacgg cctggagacc tcgatcgacg gccggccgac cgacgcgctg cactattcgc
      841 tgggctacac ctacaccaac gcccggctgg tcgatgacgt ggtcagcccg ctcggggccg
      901 tgctcggaac ggacggctcc cacctgcccg gcacgccgga gcacgccatc accctggcgg
      961 cggactacac ctggtcgctc gccggggacg tgacgctggt ggcgcggggc aacggctact
     1021 accagtccga gacccgcaac gcgatcggcg gccccaagta caataccgag ctggccggct
     1081 tcaccatctg gaacggctcg gccaccctgt ccaggggccc ctgggccgcc accttcttcg
     1141 tcaagaacat cttcaacgag gaaggcgtga cgggtgagtt caccgaggcc tacatgggca
     1201 cgcggccggc cgtcggctac tacggcaacg ggtccaagga gttcatctcg ctcccgcgca
     1261 ccctcggcct gtcgatcgac ttcagcttct agactcggga tggccagcct ggcgaccagg
     1321 gacgacgtta tgggtgagct gggcggcgtc gctccgctca tccgtgacgg tcgcctgggc
     1381 gacgccctcg ccaggctggc ctctcttgag acccgcgccc acgacgacgc ccgtctgctg
     1441 cagcacctgg gcgagcactt cacgcactgc ggccggtcgg cggacgcggc gcgctgctac
     1501 cggcgggcgg cggcgttgac cccgaacgat ccgcggttgc tctacaacct ggccacggcc
     1561 ctgatcgccc tgggccagac ggccgaggcc gaggccctgc tggaccaggt catcctgctc
     1621 gatcccgacg accacgacgc ctggcagaac cgctcgacgc tgcggcgcca gactcagcag
     1681 tcgaaccaca ccgcggcgct gctccaggcc acccgccagc gcctgaccgg cccgggcgag
     1741 gtcgcgctga gctacgccct ggccaaggag ctcgaggatc tcggcgagca tgagttgagc
     1801 ttcgcctggc tcaggcgggg ggccgaccgc cgccgcagcc tgctgagcta tcaggtcgag
     1861 gccgatgtcg ccgccatggc cgagatcgcg cgcgtgttcg acgcggccct gttctcatct
     1921 cccgtaccgg gctgtgacga gccggggccc gtcttcgtgc tcggcctgcc gcgcagcggc
     1981 acgaccctgg tggaccgcat catcagctcg cacagcgacg tggagagcct gggcgagatc
     2041 aatgacttcg cgtacgccct gatgcgcgtc gccggaccct ccgcggccaa gggcgacctg
     2101 atccggcgct cggcgcgcct cgatccggcc gcgctcggga gggcctacct cgacagcgtc
     2161 gccggccgcg gccggtcggc gcccctgttc atcgacaaga cgccggtcaa cttcctctac
     2221 atcggcctga tcgcccgcg
//
LOCUS       DAORNX010000473         2238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36292_length_2238_cov_4.242785, whole genome shotgun sequence.
ACCESSION   DAORNX010000473 DAORNX010000000
VERSION     DAORNX010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36292_length_2238_cov_4.242785"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLO65_15410"
     CDS             complement(<1..318)
                     /locus_tag="PLO65_15410"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRD29688.1"
                     /translation="MIIDEALPAARSAGLVDIVDAYLEGIAFTPAEVSRLFDKAAALG
                     LPVRLHADQLSDGGGAALAASHGALSADHLEFTSQAGIDAMARAGTVAVLLPGAFVAL
                     AETH"
     gene            311..898
                     /locus_tag="PLO65_15415"
     CDS             311..898
                     /locus_tag="PLO65_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29689.1"
                     /translation="MIMVLVFRHGVQAEEAGHHPRVAVEIAGRAQHLQLGLEVQAVAG
                     LDLKGRDALGDEGRQPPAGGLDQPGLGRRARGRHGRADTAAGQGDLLVGRAVQPRLVL
                     ADAVAAEHQVRVAVDQARRDPAAVQPRHAGRLDLRQITPRADPQDPAVLHGDRRVLDR
                     AIGRAAGRHGRGAAVGDEKVDPLHRPVLPSGPRRE"
     gene            929..>2238
                     /locus_tag="PLO65_15420"
     CDS             929..>2238
                     /locus_tag="PLO65_15420"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HRD29690.1"
                     /translation="MTDNLHLDLARLPDGWARDVRITVADGVITGLAVGAEPAGQRLR
                     GAVIPAITNLHSHAFQRAMAGLAERARAGVEDFWAWREAMYRFLDRLTPPQIEAIAAM
                     LYAELAEGGYGGIVEFHYLHNDPDGQAYADPAETAHAHRRAAATAGFGLTLAPVLYSH
                     AGFGGLPPKHGQRRFIKTTDQYLRLLEASGAEAMAFHSLRAVTPEQIGAVLDAADPAL
                     PIHIHAAEQTGEVDDCLAWSGARPVEWLLANTPVDGRWTLVHATHMTDAEATGLARGG
                     ATVALCPSTEGNLGDGFFNADAFLGAGGTFGIGSDSHVTTSAAEELRWFEYGRRLRSR
                     KRTMSGGTGEHVGAGLWLSAARAGTRPTGRGTGEIAVGARCDLVVLDTDHPTLAGRVD
                     DLILDSLVFAGSGAIDQVWSAGTRIVEGGRHVARDAIAAAYRKAV"
BASE COUNT          315 a          830 c          787 g          306 t
ORIGIN      
        1 gtgcgtctcg gccagggcca cgaaggcgcc cggcagcagc accgcgaccg ttccggccct
       61 ggccatggcg tcgatcccgg cctgcgaagt gaactccaga tggtccgccg aaagggcgcc
      121 atgggatgcc gccagcgccg cgccgccacc gtccgacagc tggtcggcgt gcagccgcac
      181 gggcaggccc agggccgccg ccttgtcgaa caggcgcgaa acctcggccg gggtgaaggc
      241 gatgccctcc agataggcgt cgacgatgtc gaccagcccg gccgaacggg cggccggcag
      301 ggcctcgtcg atgatcatgg tcctggtctt tcggcacggc gtgcaggccg aggaagctgg
      361 tcaccacccg cgcgtcgccg tcgagatagc gggccgcgcg cagcatcttc agctcggtct
      421 cgaggtccag gccgtagccg gacttgatct caagggtcgt gacgccctcg gcgacgaagg
      481 ccgccagccg cctgcgggcg gccttgatca gcccggcctc ggtcgccgcg cgcgtggccg
      541 ccacggacga gcggataccg ccgccggcca gggcgatctc ctcgtaggtc gcgccgttca
      601 gccgcgcctc gtactcgccg atgcggtcgc cgccgaacac caggtgcgtg tggcagtcga
      661 tcaggcccgg cgtgacccag cggccgtcca gcctcgccat gccggccgtc tggacctccg
      721 gcagatcacc ccgcgggccg atccacagga tcctgccgtc cttcacggcg atcgccgcgt
      781 cctcgatcgc gccataggcc gcgccgccgg ccgtcatggt cgcggcgcgg cagtcggtga
      841 tgagaaggtc gatccgctgc atcgccctgt cttgccatcg gggccgcgtc gtgaatagtc
      901 tgcacctgtc tagacaggat tagacaactt gaccgacaac cttcacctcg acctggcgcg
      961 gctgcctgac ggctgggcgc gcgatgtgcg gatcaccgtc gcggacgggg tgatcaccgg
     1021 cctggccgtc ggcgccgagc cggcagggca gcgcctcagg ggcgcggtca tcccggccat
     1081 caccaacctg cacagccacg ccttccagcg cgccatggcg ggcctggccg agcgggcgcg
     1141 ggccggggtc gaggacttct gggcctggcg cgaggccatg taccgcttcc tcgaccggct
     1201 gacgccgccg cagatcgagg ccatcgccgc catgctttac gccgagctgg ccgagggcgg
     1261 ctacggcggc atcgtcgagt tccactacct gcacaacgac ccggacggtc aggcctacgc
     1321 cgacccggcg gagacagctc acgcgcaccg ccgggccgcc gcgacggcgg gcttcgggct
     1381 gaccctggcc cccgtgctct acagccatgc cggcttcggc ggtctgccgc cgaaacacgg
     1441 ccagcgccgc ttcatcaaga ccactgacca atacctgcgc ctgctggagg ccagcggcgc
     1501 cgaggccatg gccttccaca gcctgcgggc cgtgacgccg gaacagattg gcgccgttct
     1561 ggacgccgcc gatcccgccc tgccgattca cattcacgcc gccgaacaga ccggcgaggt
     1621 cgacgactgc ctggcctggt ccggcgcccg gcccgtcgaa tggctgctgg ccaatacgcc
     1681 ggtcgatggc cgctggaccc tggtgcacgc cacccacatg accgacgccg aggcgacggg
     1741 tctggcccgc ggcggcgcga ccgtggccct ctgtccgtcg acggagggca acctcggcga
     1801 cggcttcttc aacgccgacg ccttcctcgg ggcgggcggg accttcggca tcggctccga
     1861 cagccatgtg accaccagcg ccgccgagga actgcgctgg ttcgagtacg gacgccggct
     1921 gcggtcgcgc aaacgtacga tgagcggcgg cacgggcgag catgtgggcg cgggcctgtg
     1981 gctttccgcg gcgagggccg ggacccggcc gacgggccgc gggacgggcg agatcgccgt
     2041 cggcgcccgc tgcgatctgg tggtgctcga cacggatcat ccgacgctgg ccggccgggt
     2101 cgacgacctg atcctcgaca gcctggtgtt cgcgggctcc ggcgccatcg accaggtctg
     2161 gtcggcgggg acgcgcatcg tagagggcgg ccgtcacgtc gcccgcgacg ccatcgccgc
     2221 ggcctaccgc aaggccgt
//
LOCUS       DAORNX010000474         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36545_length_2226_cov_8.856748, whole genome shotgun sequence.
ACCESSION   DAORNX010000474 DAORNX010000000
VERSION     DAORNX010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36545_length_2226_cov_8.856748"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLO65_15425"
     CDS             complement(<1..526)
                     /locus_tag="PLO65_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29691.1"
                     /translation="MAKAVFQKNQKVWVESVGAWATIDRIVPIWAKGFDEPVRVTYDV
                     GLGREFHAHELRPEDRVEDADGAMPGEGWRLMRAKNKWQQEGDCAHHPFPGTYPVVVT
                     DPADWGGWRTPGAEYDRDPNKIERQARLIASAPRLQAIARELIAMVSEAPEDAPPAVA
                     ELARRAAMIERYVQE"
     gene            775..1890
                     /locus_tag="PLO65_15430"
     CDS             775..1890
                     /locus_tag="PLO65_15430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29692.1"
                     /translation="MNFDYSDDQKFLKNEARKFLEARCTSAAVRKVLDNDDVSFDAEL
                     WKGVGEQGWLGAAIPEEFGGLGLGHVELCAIAEEMGRVVAPIPFASTVYFLAEAVLLA
                     GDDAQKADLLPKIAAGELIGCFATSEGPGVVSPSSLACRVESGKLSGVKIPVTDGDIA
                     DVAVVLASEGGKPGLFLVDLKGAGVSRETLKSLDPTRGVARLTFKDAAATRLGGAGEG
                     FALTEQVFDRAAVLLAFEQTGGADKCLEMAREYALGRYAFGRVIAGYQAIKHKLADMY
                     VKNQVARSNAYYGAWALNTNAAELPVAAAAARIAACEAYWFGSKENIQTHGGMGFTWE
                     VDCHLYYRRSRQLSLVAGAPKVWKERLVSQLERRNAA"
     gene            2036..>2226
                     /locus_tag="PLO65_15435"
     CDS             2036..>2226
                     /locus_tag="PLO65_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD29693.1"
                     /translation="MDFNDSPEEAAYREKARAWLETNAAAHKGAASANGRRPNSPEHM
                     AMAKAWQAKKADAGYACIT"
BASE COUNT          370 a          788 c          732 g          336 t
ORIGIN      
        1 cctcctggac atagcgttcg atcatcgccg cgcgacgcgc cagttcggcg acggccggcg
       61 gcgcgtcctc cggcgcctcg gacaccatgg cgatgagttc gcgggcgatg gcctgcagcc
      121 gcggggcgct ggcgatcagc cgggcctgtc gctcgatctt gttggggtcg cggtcatatt
      181 cagcgcccgg cgtgcgccat ccgccccaat cggccggatc ggtgaccacg accggatagg
      241 tgccggggaa cgggtggtgg gcgcagtcgc cttcctgctg ccacttgttc ttggcccgca
      301 tcagccgcca gccctcgccg ggcatggcgc cgtcggcgtc ctcgacgcga tcctcgggcc
      361 gcagctcatg ggcgtgaaac tcgcggccca gacccacatc gtaggtcacc cgcaccggct
      421 cgtcgaagcc cttggcccag atcggcacga tcctgtcgat cgtcgcccag gccccgacgc
      481 tctcgaccca caccttctgg ttcttctgga atacggcctt ggccatgcac ccgcgccatc
      541 gttctgaatt cagccgccta ttaagaacct gaattcattg acgcgaggtc aatctttccc
      601 cgcccgtaaa gccagctaca ttgccgctcc gaaggttcgc acaaacgccc tgcgtccttc
      661 gacacgccgc tttcgcggct gctcaggatg acgtgcaacg ggtctacgtc atggtgagca
      721 gcacccggag ggtgcgtgtc gaaccacgca tagaggcgct accgggagag agacgtgaat
      781 ttcgactatt ccgacgacca gaagttcctc aagaacgagg cgcgcaagtt cctcgaggcg
      841 cgctgcacca gcgccgccgt gcgcaaggtg ctcgacaacg acgacgtctc gttcgacgcc
      901 gagctgtgga agggcgtggg cgagcagggc tggctcggcg cggcgatccc cgaggaattc
      961 ggcggcctgg gcctgggcca tgtcgaactc tgcgcgatcg ccgaggagat gggccgcgtg
     1021 gtggcgccga tcccgttcgc ctcgaccgtc tatttcctgg ccgaggccgt gctgctggcc
     1081 ggcgacgacg cccagaaggc cgaccttctg ccgaagatcg ccgccggcga gctgatcggc
     1141 tgcttcgcca cctccgaggg cccgggcgtc gtcagcccgt cctcgctggc ctgccgggtc
     1201 gagagcggca agctgagcgg ggtgaagatc ccggtgaccg acggcgacat cgccgatgtg
     1261 gcggtcgtgc tggccagcga gggcggcaag cccggcctgt tcctggtcga cctgaagggc
     1321 gccggggtca gccgcgagac gctcaagagc ctcgacccga cccgcggcgt ggcccgcctg
     1381 accttcaagg acgccgccgc cacccgtctc ggcggggccg gcgagggctt cgccctgacc
     1441 gagcaggtgt tcgaccgggc tgccgtgctg ctggccttcg aacagaccgg cggcgccgac
     1501 aaatgcctgg agatggcccg tgaatacgcc ctgggccgct acgccttcgg ccgcgtcatc
     1561 gccggctacc aggcgatcaa gcacaagctg gccgatatgt acgtcaagaa ccaggtcgcc
     1621 cgttccaacg cctactacgg ggcctgggcg ctcaacacca acgccgccga actgccggtc
     1681 gctgccgcgg cggcgcggat cgcggcctgc gaggcctact ggttcggcag caaggaaaac
     1741 atccagaccc acggcggcat gggcttcacc tgggaggtgg actgccacct ctactaccgc
     1801 cgctcgcgcc agctgagcct ggtcgccggc gcgccgaagg tctggaagga acggctggtc
     1861 agccagctcg aacgcaggaa cgcggcctga acccctctcc cctagcggga gagggtggcc
     1921 cgaagggccg ggtgaggggt cacaccggct ttttcgctaa cagctttcga cccctcatcc
     1981 gtcggcctcg ccgacacctt ctcccgcagg gggagaagga aacgggacaa agaccatgga
     2041 tttcaacgat agccccgaag aagccgccta ccgcgagaag gcccgcgcct ggctcgagac
     2101 caacgccgcc gcccacaagg gcgcggccag cgccaacggc cgccggccca acagccccga
     2161 gcacatggcc atggccaagg cctggcaagc caagaaggcc gacgccggct acgcctgcat
     2221 cacctg
//
LOCUS       DAORNX010000475         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36798_length_2214_cov_9.055118, whole genome shotgun sequence.
ACCESSION   DAORNX010000475 DAORNX010000000
VERSION     DAORNX010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36798_length_2214_cov_9.055118"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLO65_15440"
     CDS             complement(<1..645)
                     /locus_tag="PLO65_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRD29694.1"
                     /translation="MAALVRAASLTGYSELVMTLGGDPRRLLAGCGLSPVMIDDPDLK
                     ISADTVGRLLERTAEQLGAPDFGLRLAEARRPSNLGPVGMVVREQASLGQALEVMRQY
                     QWLHNEALALTIEEEGDIAVLSLALLARGGLASRQATELSVAVLARLLSTLLGGGWRA
                     ESAHFRHSAPPSLVTHRRVLSVSPAFDQPFAARAAAEGVVTLAALDRIAVVAAFA"
     gene            736..1110
                     /locus_tag="PLO65_15445"
     CDS             736..1110
                     /locus_tag="PLO65_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD29695.1"
                     /translation="MTEPTPITDALRASGEFNPAWEAIADSDPAWLESFLAMGVRPLR
                     SGVLDPKTFELIAIAVDASCTHLYAPGVRRHIRKALQIGVSPSEIAAVLEAVAVLGIH
                     SCALGFPILAEELAAREAAQPA"
     gene            1129..2178
                     /locus_tag="PLO65_15450"
     CDS             1129..2178
                     /locus_tag="PLO65_15450"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRD29696.1"
                     /translation="MHFLDDALLPENQQPLVIQAAPYGPEFLPGDSDDIPVTMDEQVQ
                     KAVDCWNAGATVLHVHVREADGSGSKRLSMFNEMLARVRAAVPDMVLQVGGSISFAPE
                     GEGDSAKWLSDDTRHMLAELDPKPDQVTIAINTNQMNITEVMSADDVAGTSLALPAYA
                     EAYREMWIPSGPAFVEEHLRRLQAAGIQPHFMLGDPGQLETVERLFRRGVYTGPLVMN
                     WVGIGGGADGPSPTNLMEFVRRSPDNAVLTVEGIMRTCLPLNTMAIAMGLHVRCGIED
                     NLWGRKGERLSSVRQIEQLVRISRELHREVASGADARRIYRLGETYASADETLARVGW
                     APNRRPAPADVARAA"
BASE COUNT          359 a          798 c          742 g          315 t
ORIGIN      
        1 ggcgaaggcg gcgacgacgg cgatacgatc aagggcggca agggtgacga caccctccgc
       61 ggcggcgcgg gcagcgaacg gctgatcgaa ggcgggggac acgctcagca cccgccggtg
      121 ggtgaccagc gatggcgggg cggaatggcg gaagtgcgcc gattccgcgc gccagccgcc
      181 gccgagcagc gtcgacagaa gccgggccag caccgccacc gacagctccg tcgcctgccg
      241 cgaggccagg ccgccccggg ccagcagcgc cagcgaaagc acggcgatgt cgccctcctc
      301 ctcgatcgtc agggccaggg cctcgttgtg cagccactgg tattgccgca tcacctcaag
      361 cgcctgtccc agcgaggcct gttcgcgaac gaccatgccg accgggccga gattggacgg
      421 ccgccgggct tccgccagcc tgagtccgaa gtctggcgcg ccgagctgct cggccgtccg
      481 ctcgagaagt cgcccgacgg tgtcggcgct gatcttcagg tcggggtcgt cgatcatgac
      541 cggcgagaga ccgcagccgg cgagcaggcg gcgcgggtcg ccgcccagcg tcatcacgag
      601 ctccgagtag ccggtcaggc tggcggcgcg aacgagagcg gccatgtgaa ctgtcccaaa
      661 ttcgacattt gctgtcccaa atattcaatc atggacaggg tcgatgaggc aacatccggc
      721 acaaggagac accccgtgac cgaaccgacg cccatcaccg acgccctgcg cgcttccggc
      781 gagttcaatc ccgcctggga ggccatcgcc gacagcgacc cggcctggct ggaatcattt
      841 ctcgccatgg gcgtccgtcc cctgcgctcg ggcgtgctcg atccgaagac cttcgaactg
      901 atcgccatcg ccgtggatgc gtcctgcacg cacctctacg caccgggggt ccgccgccac
      961 atccgcaagg ccctgcagat cggggtgtcg ccgtcggaaa tcgccgccgt gctcgaggcc
     1021 gtcgcggttc tcggcatcca ttcctgcgcc ctcggcttcc cgatcctcgc cgaggagctc
     1081 gccgcccggg aggcggctca acccgcctga atcaacggag gagttccaat gcatttcctc
     1141 gacgacgccc tgctgccgga aaaccagcag cccctggtca tccaggccgc cccctacggt
     1201 cccgagttcc tgccgggcga ttccgacgac atccccgtga ccatggacga gcaggtccag
     1261 aaggcggtcg actgctggaa cgccggcgcg accgtcctgc acgtccatgt ccgcgaggcc
     1321 gatggctcgg gttccaagcg cctgtcgatg ttcaacgaga tgctcgcccg tgtccgggcg
     1381 gccgtgcccg acatggtgtt gcaggtcggc ggctcgatct ccttcgcgcc ggagggcgag
     1441 ggtgactcgg cgaagtggct gtccgacgac acgcgtcaca tgctcgccga gctcgacccg
     1501 aagcccgacc aggtgaccat cgccatcaac accaaccaga tgaacatcac cgaggtgatg
     1561 agcgccgatg atgtcgccgg cacctcgctg gcgctgccgg cctatgcgga agcctatcgc
     1621 gagatgtgga tcccgtccgg cccggctttc gtcgaggagc acctgcgccg gctgcaggcg
     1681 gcgggcatcc agccccactt catgctcggc gatcccggcc agctggaaac cgtcgagcgc
     1741 ctgttccgcc gcggcgtcta caccggaccg ctggtgatga actgggtcgg catcgggggc
     1801 ggcgccgacg gcccgagccc gaccaacctg atggagttcg tccgccggtc gccggacaac
     1861 gcggtgctga ccgtggaggg catcatgcgc acctgcctgc cgctcaacac catggccatc
     1921 gccatggggc tgcacgtgcg ctgcggcatc gaggacaacc tctggggccg caagggtgag
     1981 cgcctgtcct cggtccgcca gatcgaacag ctggtccgca tctcgcggga gctccaccgg
     2041 gaggtcgcca gcggcgccga cgcgcgacgc atctaccgtc tgggcgagac ctacgcctcg
     2101 gccgacgaga ccctggcccg ggtgggctgg gcccccaacc gccgtcccgc gcctgccgac
     2161 gtcgccaggg cggcctgatc cggtgacgga ccgtgccgcc gacgacgggg gcga
//
LOCUS       DAORNX010000476         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36909_length_2208_cov_5.899675, whole genome shotgun sequence.
ACCESSION   DAORNX010000476 DAORNX010000000
VERSION     DAORNX010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36909_length_2208_cov_5.899675"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLO65_15455"
     CDS             complement(<1..258)
                     /locus_tag="PLO65_15455"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29697.1"
                     /translation="MTFNAITAVQSLMDEVHRRAPEIESVRRLPADLAKAFADAGLFR
                     MLLPASLGGHDTPPAQIALAVETLAQADASAAWCLMIGATTG"
     gene            complement(255..1412)
                     /locus_tag="PLO65_15460"
     CDS             complement(255..1412)
                     /locus_tag="PLO65_15460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29698.1"
                     /translation="MIAEPDHVRELRSILQRFIAEKAPREKRRDWDRTHTFPRDLFRE
                     LAALGLCGLTVPEEYGGTGPDILAAVAAIEELCRAGSFLAGPFIQCAFYGGMNILENG
                     SAEQKAELLPKLARGELFFAYGLSEPDVGGDLSSVTTRARREGDEIVITGAKRWCTGA
                     DFSDYIYCLVRSDADAPARQALSFVLIPTNAPGVTITPIDHVNLRYTLSSDVHFDEVR
                     LPLSAIVGGPERWNQGWKMLAGRALDIEKLEISACAFGLAQAALEEAWQYAQDRVQFG
                     KPISGHQAVRHALVDAKTRLEACRLMLWNAARLADAGQPCAAETSMAKLFIAETGVEI
                     ALACQKIMGAYAMSADYDIERNVRDLLGMPIVGGSSNMQRNNLAALWKLPG"
     gene            complement(1591..>2208)
                     /locus_tag="PLO65_15465"
     CDS             complement(1591..>2208)
                     /locus_tag="PLO65_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD29699.1"
                     /translation="ENRAFCAGGDFDWFDEQINDPRKFRAIAYDAKRIVSGLLDLEKP
                     IIARLNGAAAGLGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGAIIWPQLIGFAK
                     AKELLMTGDLLTAREAVGLGLINHAVPAADLDAKVAEIAGKILGNPRWAVRWTKMTAN
                     ITLKQIMVSLSDAAVAYESLSNMTADRKEAVAAFRERRPMKLTGE"
BASE COUNT          324 a          736 c          790 g          358 t
ORIGIN      
        1 ccctgtggtg gcgccgatca tcaggcacca ggcggccgag gcgtcggcct gggcgagggt
       61 ctcgacggcc agcgcgatct gcgccggcgg cgtgtcatgg ccaccgaggc tggccggcag
      121 cagcatgcgg aacaggccgg cgtcggcgaa cgccttcgcc aggtcggccg gcaggcggcg
      181 cacgctctcg atctcgggcg cgcggcgatg aacctcgtcc atcagggact gcacggcggt
      241 gatggcgttg aaggtcatcc aggaagcttc caaagggcgg cgaggttgtt gcgctgcatg
      301 ttcgaggagc caccgacgat cggcatgccc agcaggtcgc gcacgttgcg ctcgatgtca
      361 tagtccgcgg acatcgcata ggcgcccatg atcttctggc aggccagagc gatctcgacg
      421 cccgtctcgg cgatgaacag cttggccatg ctggtctcgg ccgcgcaggg ctggccggcg
      481 tcggcgaggc gggcggcgtt ccacagcatc aggcggcagg cctccagcct ggtcttcgcg
      541 tcgaccagcg cgtggcgcac ggcctggtgg ccgctgatcg gcttgccgaa ctggacgcgg
      601 tcctgggcgt actgccaggc ttcctccaac gcggcctggg cgaggccgaa ggcgcaggcg
      661 ctgatctcca gcttctcgat gtcgagcgcc cggccggcga gcatcttcca gccctggttc
      721 caccgctccg gcccgccgac gatggccgac agcggcaggc gcacctcgtc aaaatggacg
      781 tcggacgaca gggtgtagcg caggttcaca tggtcgatcg gggtaatggt cacgccgggc
      841 gcgttggtcg ggatcagcac gaagctgagc gcctggcggg ccggggcgtc ggcatcgctg
      901 cggaccaggc agtagatgta gtcgctgaag tccgcgcccg tgcaccagcg cttggccccg
      961 gtgatgacga tctcgtcgcc ctcgcgccgg gcgcgggtgg tgacgctgga caggtcgccg
     1021 ccaacgtcgg gttccgacag gccgtaggcg aagaacagtt cgcccctggc cagcttcggc
     1081 agaagctcgg ccttctgttc ggccgagcca ttctcgagga tgttcatccc gccgtagaag
     1141 gcgcactgga tgaacggccc ggcgaggaac gatcccgccc ggcacagctc ttcgatggcc
     1201 gcgacggcgg ccaggatgtc cgggcccgtg ccgccatatt cctccggcac cgtcaggccg
     1261 cagaggccga gggcggccag ctcgcggaac aggtcacgcg ggaaggtgtg ggtgcggtcc
     1321 cagtcgcggc gtttctcgcg tggggccttc tcggcgatga agcgctggag gatcgagcgc
     1381 agctcgcgga cgtggtcggg ttcggcaatc atttcccttc tccccttgcg ggagaaggtg
     1441 gcggccgaag gccgacggat gaggggtcat aggtcgaagt cgcctcgccc gaccagccgc
     1501 aatccgccgt cagtctggga aacccctcat ccgtcaggct tcgcctgaca ccttctcccg
     1561 caaggggaga agggcgcgat gcccgcccca tcactccccc gtcagcttca tcggccggcg
     1621 ctcgcggaag gcggcgacgg cctccttgcg gtcggcggtc atgttggaga gggactcgta
     1681 ggccaccgcc gcgtcgctga ggctgaccat gatctgcttg agcgtgatgt tggcggtcat
     1741 cttggtccag cgcaccgccc agcgcgggtt gccgagaatc ttgccggcga tctccgccac
     1801 cttcgcgtcg agatcggcgg cggggaccgc atggttgatc aggcccaggc cgaccgcctc
     1861 ccgggcggtc agcaggtcgc cggtcatcag cagctccttg gccttggcga agccgatcag
     1921 ctgcggccag atgatcgccc cgccgtcgcc ggcgaccagg cccaccttca catgcgggtc
     1981 gccgatcttc gcggtctcgt cggcgatgat cacgtcgcac agcagggcga tggtcgcgcc
     2041 gagcccggcc gcggcgccgt tcaggcgggc gatgatcggc ttttccaggt ccagcaggcc
     2101 gctgacgatg cgcttggcgt cgtaggcgat ggcgcggaac ttccgcggat cgttgatctg
     2161 ctcgtcgaac cagtcgaagt cgccgccggc gcagaaggcg cggttctc
//
LOCUS       DAORNX010000477         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_36935_length_2207_cov_5.021840, whole genome shotgun sequence.
ACCESSION   DAORNX010000477 DAORNX010000000
VERSION     DAORNX010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36935_length_2207_cov_5.021840"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1459
                     /locus_tag="PLO65_15470"
     CDS             137..1459
                     /locus_tag="PLO65_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD29700.1"
                     /translation="MPHDLTAFRRGDAGYEPARRACSFNARLPDRFPEIIVQARSQAD
                     VIAAVKLAGTNGWTIGVRSGGHSWAANHVRDGGMLLDVSGLDAVEIDAAAMRARVGPG
                     CKGNLVNEQLAKRRLFFPVGHCESVCLGGYLLQGGFGWNSRVLGNACENVVGIDYVDA
                     SGEVRHASAEENADMFWAARGAGPGFFAVVTRFHLKLTRRPRVIGAKAAFYGVEHLEA
                     LVRWAHTVGPEVPASIELMMLMSRKTDFVKGPGIAVVAPVFADSLRQAWRDLAFMKTL
                     PPGATRVLPFLPTSVKALTRQVMGHYPTGFNYAVDNMWTRAPAEALLPGLRRIADTLP
                     VAPSHMLWMNWAPPASRPDMAYSLDDKIYIALYGVWRDEADTPTAATWAVDNMRAMAP
                     LSMGVQLADENLGARPAPFLADANMARLDALRAKHDPDGRFHPWMGRV"
     gene            1459..>1787
                     /locus_tag="PLO65_15475"
     CDS             1459..>1787
                     /locus_tag="PLO65_15475"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29701.1"
                     /translation="MAGQLGWSQAEIATLPWARFWNPDMAPLPQRAVRALERGQLAPP
                     LLPPLAESGPGLMGDHDGPDDGYALTPGGGVRVNIRTDMPGVTPEMIDWWFGWHGDAA
                     FTAPVGSR"
     assembly_gap    1788..1927
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1928..>2207
                     /locus_tag="PLO65_15480"
     CDS             <1928..>2207
                     /locus_tag="PLO65_15480"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29702.1"
                     /translation="VDPAGCGFTDPRLADPEQATLVLARTGLAGAPFNVGWLAHCAVR
                     TGEGSAVRSRFWFGRPHVATRDGPVGAVVAATAGRLARISEGEARSILI"
BASE COUNT          272 a          766 c          732 g          297 t
ORIGIN      
        1 gtggcgttaa acgtcatcgc cgccgacacc gacgacgaag cccgccgcct cgcggacgcc
       61 gacatggacg tcgtcacgcc gccgcccctg cccctgcgct ggcaggcggt cccggcgccc
      121 ttccaggagg ccctccgtgc cgcatgacct gaccgccttc cgccgtggcg acgccggcta
      181 cgaacccgcc cgccgcgcct gttcgttcaa cgcccgcctg cccgaccgat ttcccgagat
      241 catcgtccag gcccgcagcc aggccgatgt catcgccgcg gtgaagctgg caggaaccaa
      301 cggctggacc atcggcgtcc gctccggcgg gcacagctgg gcggccaatc atgtgcgcga
      361 cggcggcatg ctgctggacg tctcgggcct ggatgcggtg gagatcgacg cggcggcgat
      421 gcgggcgcgg gtcgggccgg gctgcaaggg aaacctcgtc aatgagcagc tggcgaaacg
      481 ccggctgttc tttcccgtgg gccactgcga gagcgtctgc ctgggcggct acctcctcca
      541 gggcggattc ggctggaaca gccgggtgct cggcaacgcg tgcgagaacg tggtcggcat
      601 cgactacgtc gacgctagcg gcgaggtccg gcacgccagc gccgaggaga acgccgacat
      661 gttctgggcc gcgcgcgggg ccggcccggg cttcttcgcg gtggtcaccc ggttccacct
      721 gaaactgacg cgccggcccc gggtgatcgg ggccaaggcc gccttctacg gcgtcgagca
      781 tctggaggcg ctggtccgct gggcgcacac ggtcgggccg gaggtccccg cgtccatcga
      841 gctgatgatg ctgatgtccc gcaagaccga cttcgtgaag ggacccggca tcgccgtcgt
      901 ggcgccggtg ttcgccgaca gcctccgcca ggcctggcgg gacctggcct tcatgaagac
      961 cctgcccccg ggcgcgacgc gggtccttcc gttcctgccc acgtcggtca aggcgttgac
     1021 ccgacaggtg atgggccact acccgaccgg cttcaactac gcggtcgaca acatgtggac
     1081 ccgcgccccg gccgaggccc tgctgccggg cctgcggcgg atcgccgaca ccctgccggt
     1141 cgcgccctcg cacatgctgt ggatgaactg ggcgccgccc gcgtcgcggc cggacatggc
     1201 ctattccctc gacgacaaga tctacatcgc cctctacggc gtctggcgcg acgaggccga
     1261 tacgccgacg gccgcgacct gggcggtcga caacatgcgg gcgatggccc cgctgtcgat
     1321 gggcgtgcag ctggccgacg agaacctcgg cgcccgcccc gcgcccttcc tggccgacgc
     1381 caacatggcc cggctcgacg cgctgcgggc gaagcacgat cccgatggcc ggttccatcc
     1441 ctggatgggc cgcgtctgat ggccggtcag ctgggctgga gtcaggccga aatcgccacc
     1501 ctgccctggg cccggttctg gaaccccgac atggcgccgc tgccgcagcg cgcggtgcgg
     1561 gcgttggagc gcggtcagct ggcgccgccg ctgctgccgc cgctggcgga gtccggcccg
     1621 ggcctgatgg gcgatcatga cgggcccgac gacggctacg ccctgacccc cggcggcggg
     1681 gtccgggtca atatccgcac cgacatgccc ggcgtcacac ccgagatgat cgactggtgg
     1741 ttcggctggc acggcgacgc cgccttcacc gcgccggtcg gcagccgnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnntgt cgacccggcc ggatgcggct tcaccgatcc gcgcctggct gaccctgaac
     1981 aggcgaccct cgtgctggcc cgcacgggcc ttgccggcgc gccgttcaac gtcggctggc
     2041 tggcgcactg cgccgtccgc acgggcgagg gcagcgcggt gcgttcacgt ttctggttcg
     2101 gccgccccca cgtcgccacc cgcgacggcc cggtcggcgc ggtcgtcgcc gccacggcgg
     2161 ggaggctggc gcgcatcagc gagggcgagg cgcggtcgat cctgatt
//
LOCUS       DAORNX010000478         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37046_length_2202_cov_5.326968, whole genome shotgun sequence.
ACCESSION   DAORNX010000478 DAORNX010000000
VERSION     DAORNX010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37046_length_2202_cov_5.326968"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLO65_15485"
     CDS             <1..406
                     /locus_tag="PLO65_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRD29703.1"
                     /translation="KLGDPGAREFIGQLPGGGRAEGVWFRMQPGAPVASPAQGVVEFA
                     GPVTGWGVILILRTTGAYHLVLAGLGEATAAPGQAVSAGAPIGRMARRGGTGNGDQAE
                     PELYLELRRSGTPVDPAPWLKARPVGIAAVGR"
     gene            488..1915
                     /locus_tag="PLO65_15490"
     CDS             488..1915
                     /locus_tag="PLO65_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRD29704.1"
                     /translation="MKKNLLIGACAFVLGAGSMAYVGQKAEATAQPRAQTYKMLELFG
                     DVLDIVQKQYVVEVDDKKLIQAALDGMVSSLDPHSGYLAPESFEDMQDTTRGEYGGLG
                     IEITSEEGVVKIISPMDGTPASRAGLQPGDYITAVNGESVIGLPTNEAVKLMRGPAGE
                     KVVLTIAREKADAFDVTLVREVIKPKSVTARLEGDYGYVRLSGFNEKATDEVVAAIKG
                     LQAQNPKMKGLVFDLRNNPGGLLDQAVGVADVFLDGGEVVSQRGRDPRDIERYNARPG
                     DMLNGLPVVVLVNSGSASAAEIVAGALQDRKRAEVVGLTSFGKGSVQTVIPLRGGADG
                     ALKLTTARYFTPSGRSIQRTGIEPNLEVASSREQAELIATRSFQFSEASLRGALDTED
                     GAVRRGAHAPAEAPPSTFDEESCKKRPPSNTLELKCDFQLQRALDVLRYGSVAATPKL
                     PKPAPRVASVVGGRVVLPGATDKPK"
     gene            1973..>2202
                     /locus_tag="PLO65_15495"
     CDS             1973..>2202
                     /locus_tag="PLO65_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRD29705.1"
                     /translation="MFSKKPVPAYAVSNAAGSGRLAQARAAALTAIQNPWLGAGGAGL
                     LFLLTLATLVAITSDPTAGAPTVRISLARAAA"
BASE COUNT          341 a          754 c          791 g          316 t
ORIGIN      
        1 aaagctcgga gaccctgggg cgcgagaatt catcggccag ctgccgggcg gcggacgggc
       61 ggagggcgtc tggttccgca tgcagcccgg cgcgccggtg gccagtccgg ctcaaggcgt
      121 ggttgaattc gctgggccgg tgacgggctg gggggtcatc ttgatcctgc gcaccaccgg
      181 cgcctaccat ctggtgctgg cggggcttgg cgaggcgacg gcggcgcccg gccaggcggt
      241 gtcggcgggc gcgccgatcg gccgcatggc gaggcgtggc ggaacaggaa atggggacca
      301 ggcggagccc gagctctatc tggaactcag gcggtcgggg acgccggttg atccggcccc
      361 ctggttgaag gcgaggcccg tcggcattgc ggcggtgggg cgttaggtca caatttcggc
      421 ccgcgtgcgg gctttaaatg aaggcgcggc gcgttcggcc gcatagggag cctccagcaa
      481 ggcgatgttg aagaagaacc tcctgatcgg cgcctgcgcc tttgtccttg gcgccgggtc
      541 gatggcctac gtcggccaga aggccgaggc cacggcccag ccgcgggccc agacctacaa
      601 gatgctggag ctgttcggcg acgtcctcga catcgtccag aagcagtatg tcgtcgaggt
      661 cgacgacaag aagctgatcc aggccgccct cgacggcatg gtctcctccc tcgatccgca
      721 ttccggctat ctcgcgcccg agagcttcga ggacatgcag gacaccaccc gcggcgaata
      781 tggcggcctg ggcatcgaga tcaccagcga ggagggggtg gtcaagatca tctccccgat
      841 ggatggcacc cccgcctcgc gcgccggcct gcagcccggc gactacatca ccgccgtcaa
      901 cggcgagagc gtgatcggcc tgccgaccaa cgaggcggtc aagctgatgc gcggtccggc
      961 cggcgagaag gtcgtgctga ccatcgcccg cgaaaaggcc gacgccttcg acgtcaccct
     1021 ggtccgcgag gtgatcaagc ccaagtcggt gaccgcccgg ctggagggcg actacggcta
     1081 tgtccgtctg tcggggttca acgagaaggc caccgacgag gtcgtggccg cgatcaaggg
     1141 cctgcaggcc cagaacccga agatgaaggg cctggtcttc gacctgcgca acaatccggg
     1201 cggcctgctc gaccaggccg tcggcgtggc cgacgtgttc ctcgacggcg gcgaggtggt
     1261 cagccagcgc ggccgcgatc cgcgcgacat cgagcgatac aacgcccggc cgggcgacat
     1321 gctcaacggc cttccggtgg tggtgctggt caactccggc tcggcctccg cggccgagat
     1381 cgtcgccggc gccctgcagg accgcaagcg cgccgaggtc gtcggcctga ccagcttcgg
     1441 caagggttcg gtgcagacgg tgatcccgct gcgtggcggc gccgacggcg ccctgaagct
     1501 gaccacggcg cgctacttca ccccctcggg ccgctcgatc cagcgcacgg gcatcgagcc
     1561 gaacctcgag gtggcctcga gccgcgaaca ggccgagctg atcgccaccc gctcgttcca
     1621 gttcagcgag gcctcgctgc gcggcgcgct cgacaccgag gacggcgccg tccgccgcgg
     1681 cgcccatgcc cccgccgagg cgccgccctc aaccttcgac gaggagagct gcaagaagcg
     1741 acccccgtcc aatacgctcg agctgaagtg cgacttccag ctccagcggg cgctcgacgt
     1801 gctgcgctac gggtcggtcg cggcgacccc caaactgccc aagccggcgc cgcgcgtggc
     1861 ctcggtggtc ggcgggcggg tggtgctgcc gggggcgacc gacaagccga aatagcggtt
     1921 cgttaaccat aaacggcgag ggtgacaggg cgtagtcctc gtcgccctcg tcatgttttc
     1981 caagaagcct gtccccgcct atgccgtcag caacgccgcc ggatccgggc ggctggccca
     2041 ggcgcgcgcg gccgccctga cggcgatcca gaatccctgg ctgggggcgg gcggcgcggg
     2101 cctgttgttt ctgctgaccc tggcgacgct ggtggcgatc accagcgacc cgacggccgg
     2161 cgcgcccacg gtgcggattt ccctggcgcg ggccgccgcc ag
//
LOCUS       DAORNX010000479         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37161_length_2197_cov_5.979458, whole genome shotgun sequence.
ACCESSION   DAORNX010000479 DAORNX010000000
VERSION     DAORNX010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37161_length_2197_cov_5.979458"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..>2197
                     /gene="flgK"
                     /locus_tag="PLO65_15500"
     CDS             409..>2197
                     /gene="flgK"
                     /locus_tag="PLO65_15500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRD29706.1"
                     /translation="MTLSSIMSSATSGLMAAQTGLRTVSDNIANVNTTGYVRKVVQQA
                     PLVSAGMGVGVDITGISRAADAFLQRASLTASASSAQAGVLAEAFDRAQALFGDPSGS
                     SSFFSRLDEVYSAFSAAAGDAASSIQRGAAVDSVTDFLNEASRISTSLKSLATEADAR
                     IGAAVDRANQLLGQIDSLNADISRAQVEGRDSTGSENIQSQLIDELSGLMDVSISTRA
                     NGGTVIRASDGTVLAGAGGAATLSYVRADGASGEIAVTLPGANGETSLRARLTSGEIR
                     GLLDLRDRELPAVGDQLSEFVTRAVDELNRAHNANSSVPAPASLTGRNTGLDLPTAVS
                     GFTGKTTIAVVGANGLLQRQVAIDFDAGTMSVDGGGPVAFGAGSFLSTLNTALGGFGS
                     ASFTNGALNLSASGGAGVAITDDATTPATKAGKGFSHFFGLNDLVTSAGYPFYDTGLA
                     ATSAHGFTAGDTISLRVADADGVRVKDVTVAVPAGGTMQDLLNALNSSTTGVGLYGAF
                     SLDADGAMTFTANAKGSSLSVLNDDTERGAGGPAISELFGIGLAQRSGRAERFEVRSD
                     IAGNPMKLALASLDLSQAAGGKPVLAVGDG"
BASE COUNT          324 a          813 c          753 g          307 t
ORIGIN      
        1 gtcatgtcgg gcgaggatgc ctctggcggg ggagaacggg tcggcgcctg ctgcgcccgg
       61 atcattcggt ccgtacaaag caacgggcgg gccagtttct ccggcgaccc gcaacctcct
      121 gtttcacaag ggctttcacc ccccggcccg gccccctccc cgaggccggc gagggcgctt
      181 tttgccgggc cgctatggac cgtgggggcc gcatcttgcc gcccgcgagg cgtcggcgcg
      241 cccgtggcgc cggtcttgcg catggttaat gcgaccgctt tgacggctgg aaataaatgt
      301 catgaatttc aacaggttag aatttgacct cgcgggcgcg gcggcgctcg cccggcaaat
      361 tacgccaccg gacccgtcag gctttgccgg gcgcagggca ggaaacgcat gaccctttcg
      421 tcgatcatgt cgtccgcgac cagcgggctg atggcggccc agaccggcct gcggaccgtc
      481 tcggacaaca tcgccaacgt caacacgacc ggctacgtcc gcaaggtcgt ccagcaggcg
      541 ccgctggtct cggcgggcat gggcgtcggc gtcgacatca ccggcatcag ccgggccgcc
      601 gacgccttcc tgcagcgggc ctcgctgacc gccagcgcca gttcggccca ggccggcgtg
      661 ctggccgagg ccttcgaccg cgcccaggcc ctgttcggcg acccctcggg ctccagctcc
      721 ttcttcagcc ggctggacga ggtctactcg gccttctccg cggcggccgg ggacgcggcc
      781 tcctcgatcc agcgcggcgc ggcggtcgac agcgtgaccg acttcctcaa cgaggcctcg
      841 cggatctcga cctcgctcaa gagcctggcg accgaggcgg acgcgcggat cggcgcggcc
      901 gtcgaccgcg ccaaccagct gctgggccag atcgactcgc tcaacgccga catcagccgc
      961 gcccaggtcg agggccgtga ctcgaccggc tcggagaaca tccagagcca gctgatcgac
     1021 gaactgtcgg gcctgatgga cgtcagcatc agcacgcggg ccaatggcgg gaccgtcatc
     1081 cgcgcctccg atggcacggt gctggccggg gccggcggcg ccgccaccct ctcctacgtg
     1141 cgcgcggacg gagcctcggg cgagatcgcc gtcaccctgc ccggcgccaa tggcgagacc
     1201 tcgctgcgcg cgcgcctcac ctccggcgag atccgtggcc tgctcgacct gcgcgaccgg
     1261 gaactgccgg cggtcggcga ccagctgtcc gaattcgtca cccgcgcggt cgacgaactg
     1321 aaccgggctc acaacgccaa ctccagcgtg ccggcgccgg ccagcctgac cggccgcaac
     1381 accggcctgg acctgcccac cgcggtgtcg ggcttcaccg gcaagacgac gatcgcggtg
     1441 gtcggggcca acggcctgct gcagcgacag gtggcgatcg acttcgacgc cggcacgatg
     1501 agcgtcgacg gcggcgggcc ggtggccttc ggcgccggca gcttcctgtc gaccctgaat
     1561 accgccctgg gcggcttcgg ctcggccagc ttcaccaacg gggccctgaa cctgtcggcc
     1621 agcggcggcg cgggcgtcgc catcaccgac gacgcgacca cccccgcgac caaggccggc
     1681 aaggggttct cgcacttctt cgggctgaac gacctggtca cctcggcagg ctatccgttc
     1741 tacgataccg gcctggccgc gacgagcgcg cacggcttca cggccggaga cacgatcagc
     1801 ctgcgggtgg ccgacgccga cggggtgcgg gtcaaggacg tgaccgtggc cgtgccggcc
     1861 ggcggcacga tgcaggacct gctcaacgcc ctcaactcca gcaccaccgg cgtgggtctc
     1921 tacggcgcct tcagcctcga cgccgacggg gcgatgacct tcaccgccaa cgccaagggc
     1981 tcgagcctgt cggtgctcaa cgacgacacc gagcgcggcg ccggggggcc ggccatctcg
     2041 gagctgttcg gcataggcct ggcccaaagg tcgggtcgcg ccgagcggtt cgaggtgcgc
     2101 agcgacatcg ccggcaaccc gatgaagctg gccctcgcct cgctggacct cagccaggcc
     2161 gccggcggca agccggtgct ggcggtcggc gacggcc
//
LOCUS       DAORNX010000480         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37162_length_2197_cov_5.788515, whole genome shotgun sequence.
ACCESSION   DAORNX010000480 DAORNX010000000
VERSION     DAORNX010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37162_length_2197_cov_5.788515"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLO65_15505"
     CDS             <1..264
                     /locus_tag="PLO65_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29707.1"
                     /translation="APPGARACVMVARGPLADHAGFGKRLDAWALKEGYAPAVRGETP
                     GGGQSARYATPDESRALVLAYYPDTGRADQPSRSILFVGWKVP"
     gene            261..776
                     /locus_tag="PLO65_15510"
     CDS             261..776
                     /locus_tag="PLO65_15510"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRD29708.1"
                     /translation="MTIPIALGPVARARNGVIGKDGKLPWRLKSDLALFKQVTLSKPV
                     IMGRKTWDSLPLKPLPGRLNVVLSKDGAFQPRGAVVCESFGEALEIAREQAAEDGAEE
                     VCVIGGAALYALALPRARRIYVTEVDAEPEGDVLFPPFDEADWTEVRREAHPAGEADD
                     HAFVFRVLERR"
     gene            850..1761
                     /locus_tag="PLO65_15515"
     CDS             850..1761
                     /locus_tag="PLO65_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD29709.1"
                     /translation="MDIELVAEGLRFPEGPIAMADGSVILTEIAGQRLTRVTPDGKTS
                     TVAETGGGPNGAAIGPDGAIYVTNNGGSFEWLEMQGLLIPGPTPASHVGGMIQRVDIA
                     SGKVETVYDSCDGKRLVGPNDLVFDRQGGMWFTDHGCSTPDGRKFGALYYARTDGSHI
                     SRQRDHFVSPNGVGLSPDEKVVYMADTNLGRLWAFDIAEPGVLAEPPPFQPGRVICNL
                     PGFQLLDSLAVEAGGKVCVATIINGGITAFDPDGTTEHYPFPDLLCTNICFGGPDMTT
                     AWITASGTGKLYRARWPRPGLKLNFNA"
     gene            complement(1751..>2197)
                     /locus_tag="PLO65_15520"
     CDS             complement(1751..>2197)
                     /locus_tag="PLO65_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD29710.1"
                     /translation="TLTLDLRPDLSIEALAGRLARPRGKDSVSNWLRKAGGLSPVAAG
                     LLREIPGDLPTGAEKLARRIKAVRLTLTGVQGLERAISSAGGVALDQVDEGLMLKALP
                     GVFVAGEMLDWEAPTGGYLLQASMASGVAAAAGMVDWLARRPPVRR"
BASE COUNT          331 a          803 c          765 g          298 t
ORIGIN      
        1 gcgcctcccg gggcgcgggc ctgcgtcatg gtcgcgcgcg gtccgctggc cgatcacgcc
       61 ggcttcggca agcgcctcga cgcctgggct ctcaaggagg gctatgcgcc ggccgtccgc
      121 ggcgagacgc ctggcggcgg ccagtcagcg cgctacgcca cccccgacga gagccgcgcc
      181 ctggtgctgg cctactatcc cgacaccggc cgcgcggacc agccgtcccg gtccatcctg
      241 ttcgtcggct ggaaggtccc atgaccatac ccatcgccct cggtcccgtc gcccgcgccc
      301 gcaacggcgt gatcggcaag gacggcaagc tgccctggcg gctgaagtcg gatctggccc
      361 tgttcaagca ggtgaccctg tcaaagccgg tgatcatggg gcgcaagacc tgggacagcc
      421 tgccgctcaa gcccctgccg ggtcggctga acgtggtgct gtcgaaggat ggcgccttcc
      481 agcccagggg cgcggtggtc tgcgagagtt tcggcgaggc gctcgagatc gcccgcgaac
      541 aggcggccga ggacggcgcc gaggaggtct gcgtgatcgg cggggcggcg ctctacgccc
      601 tggccctgcc ccgggccagg cgaatctacg tcaccgaggt cgacgccgag ccggagggcg
      661 acgtgctgtt cccgcccttc gacgaggcgg actggaccga ggtgcgccgc gaagcccatc
      721 cggccggtga agccgacgac cacgccttcg tcttccgggt gctcgaacgc cgctaggcca
      781 aggccgcttg cgctgacgcg gcgtcacgcg gttctatccc ggcaacggga aaagcaggga
      841 gcgcgccgca tggacatcga actcgtcgcc gaggggctac gcttccccga aggtcccatc
      901 gccatggccg acggctcggt gatcctgacc gagatcgccg gccagcggct gacccgcgtc
      961 acgccggacg gcaagacctc gaccgtggcc gagaccggcg gggggccgaa cggcgcggcg
     1021 atcggccctg acggcgcgat ttacgtgacc aacaacggcg gcagcttcga atggctggag
     1081 atgcagggcc tgctgattcc cggcccgacg ccggccagcc acgtcggcgg catgatccag
     1141 cgcgtcgata tcgccagcgg caaggtcgag acggtctacg acagctgcga cggcaagcgg
     1201 ctggtcgggc ccaacgacct ggtgttcgac cggcagggcg gcatgtggtt caccgaccat
     1261 ggctgctcca cgccggacgg ccgcaagttc ggcgcgctct actacgccag gaccgacggc
     1321 agtcacatca gccgccagcg cgaccacttc gtctcgccca acggcgtggg cctctcgccg
     1381 gacgagaagg tcgtctacat ggccgacacc aacctcggcc ggctgtgggc gttcgacatc
     1441 gccgaaccgg gcgtgctggc cgagccgccg ccgttccagc cgggccgggt gatctgcaac
     1501 ctgccgggct tccagctgct cgacagcctg gccgtcgagg ccggcggcaa ggtctgcgtg
     1561 gcgaccatca tcaacggcgg catcaccgcc ttcgacccgg acggcacgac cgagcactat
     1621 ccgttcccgg atctgctgtg caccaacatc tgcttcggcg ggccggacat gaccaccgcc
     1681 tggatcaccg cctccggcac cggcaagctc tatcgggcca gatggccgcg gcctggtctg
     1741 aagctgaact tcaacgcctg accggcgggc ggcgggccag ccagtcgacc atacccgccg
     1801 cggccgccac gcctgacgcc atcgacgcct gcagcagata gccgcccgtc ggggcttccc
     1861 agtcgagcat ctcgccggcc acgaagaccc cgggcagggc cttgagcatg agcccttcat
     1921 cgacctggtc gagggcgacg ccgccggccg aggagatggc ccgctccagt ccctggacac
     1981 cggtcagcgt caggcggaca gccttgatgc gccgggccag cttctcggcg cccgtgggca
     2041 ggtcgccggg gatctcccgc agcaggccgg cggcgaccgg cgacaggccg cccgccttgc
     2101 gcagccagtt gctgacgctg tccttgccgc gcggccgcgc cagccggccg gccagggcct
     2161 cgatcgacag gtcgggccgc aggtcgaggg tcagggt
//
LOCUS       DAORNX010000481         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37332_length_2189_cov_5.557170, whole genome shotgun sequence.
ACCESSION   DAORNX010000481 DAORNX010000000
VERSION     DAORNX010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37332_length_2189_cov_5.557170"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLO65_15525"
     CDS             complement(<1..143)
                     /locus_tag="PLO65_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HRD29711.1"
                     /translation="MIANDDWAVAAEQRVLDAALRLAPTRGWGRAMTLAAVKAAGLSE
                     AEG"
     gene            312..551
                     /gene="rpsU"
                     /locus_tag="PLO65_15530"
     CDS             312..551
                     /gene="rpsU"
                     /locus_tag="PLO65_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRD29712.1"
                     /translation="MVQIFVRDNNVDQALKALKKKMQREGSFREMKRHVHYEKPSEKR
                     ARQKAEAVRRARKLARKRLQREGLIAAPKKPAPRG"
     gene            complement(636..>1715)
                     /locus_tag="PLO65_15535"
     CDS             complement(636..>1715)
                     /locus_tag="PLO65_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD29713.1"
                     /translation="VVEQLTATTRGKPAATVVDGPVDPATPLEPRLRRWMTDGFPEAG
                     DPKLGAAAVIHGSGAAVTIDFWSSNLTDRTLERAVSTAVGEQMRARRLAALGVDPAAL
                     EAAQALAPKVASFSPKAAGGKVALRDRLPGIVGFGAAMLLWMMIFTGAGILLNSVIEE
                     KSNRVLEVLLASASAPEIMFGKILGVAGVTLTVLAVWASVGGTLLFTLQPAVAADVAA
                     VLLGKGLVAYFLFYLVGGYLMYAAIFIAIGSFCETTREAQTLLGPIMALLTIPVIFLT
                     QAIRRPDSPMLEALSWFPPFTPFLMTARAASEPPLWQVLGTGVLMAATVALVVWIAGR
                     AFRTGALSTGKIDLKGLILRVRGGE"
     assembly_gap    1716..1729
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1730..1835)
                     /locus_tag="PLO65_15540"
     CDS             complement(<1730..1835)
                     /locus_tag="PLO65_15540"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29714.1"
                     /translation="MLMMPVVGAISFGAPAWLEKTSPPPTIMIVDRSGE"
     gene            complement(1904..>2189)
                     /locus_tag="PLO65_15545"
     CDS             complement(1904..>2189)
                     /locus_tag="PLO65_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HRD29715.1"
                     /translation="VKPHGALANLAADDRAVADAITGAVAALPGVESVTTEAIEDGGT
                     RFTATLGKGAAGQDALRAALAGGHDIHRFEMREPSLHDAFIALTGGESAQ"
BASE COUNT          354 a          793 c          732 g          296 t
ORIGIN      
        1 tcgccctcgg cctccgaaag cccggccgcc ttcaccgcgg ccagggtcat ggcccggccc
       61 cagccccgcg tgggggccag ccgcaacgcc gcgtccagca cccgctgctc ggccgccacg
      121 gcccagtcgt cattcgcgat catggcccta tcctataccg ccccgcgcgc gccgtcgccg
      181 atagacaagc ccgcccgcct ctgctatctc gcgcgcctcg ccacgggagg ctcctcccgg
      241 ggccatatca tttttgattt cgtccgccac gccgcacggc tagcggcggg ccgtacgaag
      301 gagagagtcc cctggttcag attttcgtcc gcgacaacaa tgtcgaccag gcgctgaaag
      361 ccctgaagaa gaagatgcag cgtgaaggct cgttccgcga aatgaagcgg cacgtgcact
      421 acgaaaagcc gtcggaaaag cgcgcgcgcc agaaggccga ggccgtccgc cgcgcccgca
      481 agctggctcg caagcgcctg caacgcgaag gcctgatcgc cgcgccgaag aagcccgccc
      541 cgcgcgggta ggccttcgac atcgagattc ggaagggccg tccgggcgac cgggcggccc
      601 ttttgctgtc cgggccatga cggctcctgg tcggatcact ccccgccgcg cacgcgcagg
      661 atcaagccct tcaggtcgat cttgccggtc gacagggctc cggtgcggaa ggcccggccg
      721 gcgatccaga ccaccagggc cacggtcgcg gccatcagca cgccggtgcc cagcacctgc
      781 cacaggggcg gctcgctggc cgcgcgggcg gtcatcagga acggggtgaa cggcggaaac
      841 cacgacagcg cctcgagcat cggggaatcg ggccggcgga tggcctgggt caggaagatc
      901 accgggatcg tcagcagcgc catgatcggc cccagcagcg tctgggcctc gcgcgtggtc
      961 tcgcagaacg agccgatggc gatgaagatg gccgcgtaca tcaggtagcc gccgaccagg
     1021 tagaacagaa aataggccac cagccccttg ccaagcagca cggccgcgac atccgccgcg
     1081 acggccggct gcagcgtgaa caacagggtc ccgccgacgc tcgcccagac cgccagcacg
     1141 gtcagggtca ccccggcgac gccgagaatc ttgccgaaca tgatctccgg cgctgaggcc
     1201 gaggccagca gcacctccag aacgcggttg gatttctcct cgatgacgct gttcagcagt
     1261 atgccggcgc cggtaaagat catcatccac agcagcatgg ccgcgccgaa gccgacgatt
     1321 cccggcaggc ggtcgcgcag ggccaccttg ccgcccgcgg ccttcgggga gaaggacgcc
     1381 accttcggcg ccaatgcctg ggctgcctcc agcgcggccg gatcgacgcc gagggccgcc
     1441 agccgccgtg cgcgcatctg ctcgccgacc gcggtgctga ccgcccgttc cagcgtgcgg
     1501 tcggtgaggt tgctgctcca gaaatcgatg gtcacggcgg cgcccgaccc gtggatgacc
     1561 gccgccgcgc ccagctttgg atcgccggcc tcggggaagc catcggtcat ccagcgcctt
     1621 agccgcggct cgagcggcgt cgccgggtcg acagggccgt cgaccactgt cgcggcgggt
     1681 ttgccgcgcg tggttgcggt gagctgctcg accacnnnnn nnnnnnnnnc ctcgccggac
     1741 cggtcgacga tcatgatcgt gggcgggggc gaggtcttct ccagccaggc cggagcgccg
     1801 aacgagatgg ccccgaccac cggcatcatc agcatcgaca gccagaatcc gaccgtcttc
     1861 acataggcca cgtactcgcg gccggcgatg cggaacaggc gcatcattgg gcgctctccc
     1921 cgccggtcag ggcgatgaag gcgtcgtgca ggctgggctc gcgcatctcg aagcggtgaa
     1981 tgtcgtggcc gccggccagg gcggcgcgca gggcgtcctg cccggccgcg cccttgccca
     2041 gggtcgcggt gaagcgggtc cctccatcct cgatcgcctc ggtcgtcacg ctttcgacgc
     2101 ccggcagggc ggcgaccgcg ccggtgatcg catcggccac ggcccggtcg tccgccgcca
     2161 gattggccag cgcgccatgc ggcttgaca
//
LOCUS       DAORNX010000482         2188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37348_length_2188_cov_7.016878, whole genome shotgun sequence.
ACCESSION   DAORNX010000482 DAORNX010000000
VERSION     DAORNX010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37348_length_2188_cov_7.016878"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1361
                     /locus_tag="PLO65_15550"
     CDS             114..1361
                     /locus_tag="PLO65_15550"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRD29716.1"
                     /translation="MPRKPTSPPASAPPPAPPPAPPPAATPVARAPWGAGGVDKPSPS
                     SPKSATRVDSQRPPRGKRLSLSEELDVYHCGIDLHSRITVYHLLDGAGTTLARGEIDT
                     SEASLGELARGIKKHTRFYIEASTGSAWCARLIEACGHQAVVVDPNRLRAISSSPKKT
                     DAHDAEMLAMLGKTGLLTPVHVRSEFTDRFRRLLAARHAIVGSRANLIRTTRSLLRSE
                     GHNLPSCDGDDFARRLEGTWGIPEGYELAVPPLVQAIDDMTTQVLEIEKAVHEVAEAD
                     PTTVRRLRAIPGVGELIATSFLALIEDPTRFRSSEEIAAYTGLAPWVNSSAGKRKPGG
                     ITKRGNSATRALLVQGAWAHVRSKKDTALKRWYYKLSERVGKKKAIVGLARKLGELMW
                     TLWKNEADYQAFPTSSRRVPAPT"
BASE COUNT          362 a          847 c          681 g          298 t
ORIGIN      
        1 gtcgtcgtcc tcgccctgcg tcgtcggccc ctctcccggc tctcgaagcc gggccccatt
       61 gatcggatga atcggccggc cttcccctcc cgcccgatca accgagccct gcgatgcccc
      121 gaaagcccac gtctcctccc gcttcagcgc cccctcccgc gccccctccc gcgccccctc
      181 ctgccgccac gccggtggcc cgtgcgccct ggggggcggg tggggtagac aagccgagcc
      241 cctcatcgcc gaagtcggcg acccgcgtgg acagtcaacg tccgccgagg ggcaagagac
      301 tgtctctctc ggaggaactc gacgtgtacc actgtggcat agatcttcac tctcggatca
      361 ccgtctacca cttgctggac ggggcaggga ccaccctggc ccgcggagag atcgacacca
      421 gcgaggcttc cttgggggag ctggcgcgcg gcatcaagaa gcacacccgc ttctacatcg
      481 aggcatccac cggctcggcg tggtgcgcgc ggctgatcga ggcctgcggc caccaggcgg
      541 tggtggtgga ccccaaccgg ctgcgggcca tctccagctc gccgaagaag accgacgccc
      601 acgacgctga gatgctggcc atgctcggca agaccgggct gctgaccccc gtgcatgtcc
      661 ggtcggagtt caccgaccgc ttccgtcgcc tgctcgcggc ccgccacgcc atcgtcgggt
      721 cccgcgccaa cctcatccgc accacccgct ccctgctgcg cagcgaaggc cacaacctgc
      781 catcctgcga cggcgacgac ttcgcccgcc gcctcgaagg cacctggggg atccccgagg
      841 gctacgagct ggccgtgccg cccctggtcc aggccatcga cgacatgacc acccaggtcc
      901 tcgagatcga gaaggccgtc cacgaggtcg ccgaagccga tccgacgacc gtccgcaggc
      961 tgcgcgccat ccctggcgtc ggcgagctca tcgccacctc cttcctcgcc ctcatcgagg
     1021 atccgacccg cttccgctcc tccgaggaga tcgcggcgta cacgggcctg gccccctggg
     1081 tgaacagctc ggcgggcaag cgaaagcccg gcggcatcac caagcgcggc aacagcgcca
     1141 cccgggcctt gctggtccag ggcgcctggg cccatgtgcg cagcaagaag gacacggcgc
     1201 tcaagcgctg gtactacaag ctctccgagc gcgtcggcaa gaagaaggcc atcgtcgggc
     1261 tcgcacgcaa gctcggcgag ctgatgtgga ccctctggaa gaacgaggcc gactaccagg
     1321 ccttccccac aagctcccgc cgggtgcccg cacccacctg agccgcccgg cgatccgcgg
     1381 ccgcccagac gcccgcgttc acccttccac ccttcaggag caagcagcgt cgaacgacca
     1441 caggtccctc cggtgagtcg agctgcgcgg agaccagtca gccttggggg gagcgatccc
     1501 gagttttcaa gggccccaga ggccgctgca ttgtgtggga cgctccgcct cgaactcgag
     1561 tatgcgcctg gcctccggtg gaggtccctg agcgcggata gatggatgag actgactgcc
     1621 ccgcaccctc cccctacgga tccacagtcg ccaacgtctc acgacgcgat gcgcccgagc
     1681 cccacccccc cgacgacaca gcatacctgg ccggctccct ccggcaaggt cgcgccaccc
     1741 tctccgcgcc tccgcttcgc tgcggcttgt gcgaggggcc cggcactcgc ttgcctacgg
     1801 tcacccggcc aggtccccaa caggtgtcgg gggtgcagga ggaaaaagag tgggtgaagc
     1861 tgcccgggac gcatgcggtg gctcagcgaa cggtatagtt gactcctacg gccgattcat
     1921 agatgccgcc ctcgctgcgc caacctctgc cctcgctacg ccaacctcag cgcgccagcg
     1981 gggggtgtcg ccgccgtcgc gcgggccacc ggccgacgcc acgcagacgc caacggcctg
     2041 aagcgaggga cagcgacggg cgagcacagc tgcgccagag gactgaacag caccatccaa
     2101 cgggcatcca ggggccccgc tacggcggcg actcccactc cagagcgcca agtcgcgggc
     2161 atccagggcg ccagggaggg accgaccc
//
LOCUS       DAORNX010000483         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37587_length_2178_cov_7.982101, whole genome shotgun sequence.
ACCESSION   DAORNX010000483 DAORNX010000000
VERSION     DAORNX010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37587_length_2178_cov_7.982101"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLO65_15555"
     CDS             <1..360
                     /locus_tag="PLO65_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRD29717.1"
                     /translation="QAPWRDALQDRVEAAGAVILGKGAPRLPQTLGFAAPGFDAQMQV
                     MGLDLAGVMISAGPACSSGKMKPSDVVAAMGRPDLAPFAIRVSGGWASAETDWVRAGD
                     AWLDLYARHAARRKEVA"
     gene            360..1829
                     /gene="sufB"
                     /locus_tag="PLO65_15560"
     CDS             360..1829
                     /gene="sufB"
                     /locus_tag="PLO65_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRD29718.1"
                     /translation="MAAVKETVQAVADLEKYQHGFVTDIAQDFAPKGLNADIVRFISA
                     KKNEPEWMLEWRLEAFERWLAMDEPEWAKVHYPTIDYQDAYYYAAPSQKAGPMSLDEV
                     DPDILETYKKLGIPLREQEVLAGVQGAPKYAVDAVFDSVSVVTTFKKELAAVGVIFCP
                     ISEAIREHPELVKKYLGSVVPVSDNYFACLNSAVFSDGSFVYIPPGVRCPMELSTYFR
                     INAKDSGQFERTLIIADKGAYCSYLEGCTAPMRDENQLHAAVVELVILEDAEIKYSTV
                     QNWYPGDPETGKGGIYNFVTKRADCREARAKVSWTQVETGSAITWKYPSCVLRGEGSS
                     GEFYSIAVTNGHQQADTGTKMIHLGANTRSRIISKGISAGKSSNTYRGLVSAHPKAVG
                     ARNFTQCDSLLIGKDCAAHTVPYIEARNGQSVFEHEATTTRLSDDQLFYCQQRGLDTE
                     SAVALLVNGFVREVLQELPMEFAVEAQKLVAISLEGSVG"
     gene            1826..>2178
                     /locus_tag="PLO65_15565"
     CDS             1826..>2178
                     /locus_tag="PLO65_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD29719.1"
                     /translation="MTLRAIDHVQIAIPVGGEDAARPFYRDLLGLTEVPKPPELAKRG
                     GCWFEGAVKVHLGVEQDFRANVKAHVAFEVDHVAGLAARAEAAGFKAQFDDDLDGFER
                     IYLYDPFGNRLEFLKP"
BASE COUNT          386 a          727 c          729 g          336 t
ORIGIN      
        1 caggcgccct ggcgcgatgc gctgcaggat cgggtcgagg ccgccggcgc cgtcatcctc
       61 ggcaagggcg cgccgcgcct gccgcagacg ctgggcttcg ccgcccccgg cttcgacgcc
      121 cagatgcagg tcatgggcct ggatctggcg ggcgtgatga tcagcgccgg cccggcctgc
      181 tcgtctggca agatgaagcc cagcgacgtg gtcgcggcca tggggcggcc ggacctcgcg
      241 cccttcgcca tccgcgtcag cggcggctgg gccagcgccg aaacagactg ggtccgggcc
      301 ggcgacgcct ggctggacct ttatgcgcgc catgccgcgc gccggaagga agtcgcgtaa
      361 tggccgccgt caaggaaacc gtgcaggcgg tcgccgatct ggagaagtac cagcacggct
      421 tcgtcaccga tatcgcccag gacttcgcgc ccaaggggct gaacgccgat atcgtgcgct
      481 tcatttcggc caagaagaac gagccggaat ggatgctgga atggcggctg gaggccttcg
      541 agcgctggct ggcgatggac gaaccggagt gggccaaggt ccactatccg acgatcgact
      601 accaggacgc ctactactac gccgcgccgt cgcagaaggc cgggccgatg agcctggacg
      661 aggtcgatcc ggacattctg gagacctaca agaagctcgg catcccgctg cgcgaacagg
      721 aagtgctggc cggggtgcag ggcgcgccga aatacgcggt cgacgcagtt ttcgacagcg
      781 tcagcgtggt gaccaccttc aagaaggagc tggccgcggt cggggtgatc ttctgcccga
      841 tcagcgaggc gatccgcgaa caccccgagc tggtgaagaa atacctcggc tcggtcgtgc
      901 cggtcagcga caactatttc gcctgtctca acagcgcggt cttcagcgac ggcagcttcg
      961 tctacatccc gccgggcgtg cgctgcccga tggagctctc gacctacttc cggataaacg
     1021 ccaaggattc cgggcagttc gagcggactt tgatcatcgc cgacaagggt gcctactgct
     1081 cctacctcga gggctgcacc gcgccgatgc gcgacgagaa ccagctgcac gcggcggtgg
     1141 tcgagttggt catcctcgag gacgccgaga tcaagtactc caccgtccag aactggtacc
     1201 cgggcgatcc ggagaccggc aagggcggca tctacaactt cgtcaccaag cgggccgact
     1261 gccgcgaagc gcgggccaag gtcagctgga cccaggtcga gaccggctcg gcgatcacct
     1321 ggaaataccc gtcctgcgtc ctgcgcgggg agggcagctc gggcgagttc tattccatcg
     1381 ccgtgaccaa cggccaccag caggccgaca ccggcaccaa gatgatccac ctgggggcca
     1441 acacccgctc gcggatcatc tccaagggca tctcggccgg caagtcgtcg aacacctatc
     1501 gcggcctggt ctcggcccat ccgaaggccg tcggcgcgcg caacttcacc cagtgcgaca
     1561 gcctgctgat cggcaaggac tgcgccgccc acaccgtgcc ctacatcgag gcccgcaacg
     1621 gccagagcgt gttcgagcac gaggcgacca ccacgcggct gtcggacgac cagctgttct
     1681 actgccagca gcgggggctg gacacggaat cggctgtggc cctgctggtc aacggcttcg
     1741 tgcgcgaggt gctgcaggag ctcccgatgg agttcgccgt cgaggcgcag aagctggtgg
     1801 cgatcagcct tgaggggtcg gtgggatgac cctccgcgcc atcgaccatg tccagatcgc
     1861 catccccgtc ggcggcgaag acgccgcgcg tccgttctat cgggacctgc tgggcctgac
     1921 cgaggttccc aagccgccgg aactcgccaa acgcggcggc tgctggttcg agggcgccgt
     1981 gaaggtccat ctcggcgtcg agcaggactt ccgcgccaat gtgaaggccc atgtggcgtt
     2041 tgaggtcgat catgtcgccg ggctcgcggc gcgggcagag gcggccggct tcaaagccca
     2101 gttcgatgac gacctcgacg gcttcgagcg catctacctc tacgacccct tcggcaacag
     2161 gctcgagttc ctgaagcc
//
LOCUS       DAORNX010000484         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37756_length_2170_cov_8.297872, whole genome shotgun sequence.
ACCESSION   DAORNX010000484 DAORNX010000000
VERSION     DAORNX010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37756_length_2170_cov_8.297872"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLO65_15570"
     CDS             <1..146
                     /locus_tag="PLO65_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD29720.1"
                     /translation="EPADCVYLDDLGINCKPAAMLGMRAIKVGDVDQALAELSAATDL
                     IFE"
     gene            complement(199..594)
                     /locus_tag="PLO65_15575"
     CDS             complement(199..594)
                     /locus_tag="PLO65_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29721.1"
                     /translation="MDRRLALATALALLPAVASANSGGEKKKGGGATYLQIKAIAATI
                     MHRDGSRGVITVECGLDAPDEKLRTLAEQSQPRLRAAYAGFIQAYASGLPAAALPDAD
                     YISRELQRLTDSTLGRKGAKLLLGTILVN"
     gene            699..1736
                     /gene="pseB"
                     /locus_tag="PLO65_15580"
     CDS             699..1736
                     /gene="pseB"
                     /locus_tag="PLO65_15580"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRD29722.1"
                     /translation="MAGFAPDNLDLDDKVILVTGGTGSFGRRFVDTVLTRYAPRKVIV
                     YSRDELKQSEMQQEMRERLPPEQLAKMRFFLGDVRDRSRLTLALRGVDIVIHAAALKQ
                     VPAAEYNPSECIQTNIIGAENVVWASLSNHVKQVVALSTDKACNPVNLYGATKLASDK
                     TFVAANNLSGDIGTRFAVVRYGNVVGSRGSVAPLFRRLLAQGATELPVTDARMTRFWI
                     TLNQGVDFVLSSLVLMRGGEIFVPKIPSMSMPDLVQAMAPGAGMRIVGIRPGEKLHEI
                     MVGADDARQTVELADRYVIEPAFVEYTRRPFGPADGGRPVEEGFSYASDNNSDWLSAE
                     RLRAMLEETGA"
     gene            1733..>2170
                     /locus_tag="PLO65_15585"
     CDS             1733..>2170
                     /locus_tag="PLO65_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD29723.1"
                     /translation="MTTGFLPYGRQTIEEDDIAAVAAALRADYLTTGPAVEAFETAFR
                     EVVGAAHAVACSNGTAALHLAMLAAGIGPGDVCIVPAITFLATANCAAYVGADVVFAD
                     VDPDSGLMTPETLAEALDRARGRPVRAVLPVHLRGDVCDLPALA"
BASE COUNT          323 a          744 c          774 g          329 t
ORIGIN      
        1 tcgagccggc cgactgcgtc tacctcgacg acctgggcat caactgcaag ccggccgcga
       61 tgctgggcat gcgcgcgatc aaggtcggcg atgtcgacca ggccctggcc gagctgtcgg
      121 ccgcgacgga cctgattttc gaatagaggc gcggggacac gcccttcagt gcatgtcccc
      181 gtgacgggcg cggccggcct agttcaccag gatcgtgccg agcagcagct tcgcgccctt
      241 ccggcccagg gtgctgtcgg tcaggcgttg cagttcgcgc gagatgtagt cggcgtcggg
      301 cagggcggcc gcgggcaggc cgctggcgta ggcctggatg aagcccgcat aggcggcccg
      361 cagccgcggc tgcgactgtt cggccagggt gcgcagcttc tcgtcgggcg cgtcgaggcc
      421 gcattcgacg gtgatcacgc cgcggctgcc gtcgcgatgc atgatggtcg cggcgatggc
      481 cttgatctgc agataggtgg cgccgccgcc cttcttcttt tcgccgccgc tgttggcgga
      541 cgcgacggcg ggcagcaggg ccagggcggt ggcgagggcg aggcggcgat ccatgcgacc
      601 agatgaccgc aacatggttg acgcggcgtt tacacattcg cgccaagcgt agcaggcggc
      661 ggattcgatc gggaatcagc gtccgcactg gagcgcccat ggccggcttc gcgcccgaca
      721 acctcgatct cgacgacaag gtgatcctcg tcaccggcgg gacgggatcg ttcggcagac
      781 gcttcgtcga cacggtgctg acccgctacg ccccgcgcaa ggtgatcgtc tattcgcgcg
      841 acgagctgaa gcagagcgag atgcagcagg agatgcgcga gcggctgccg ccggagcagc
      901 tggcgaagat gcgcttcttc ctcggcgacg tgcgcgaccg ttcgcgcctg accctggccc
      961 tgcgcggcgt cgacatcgtg atccacgccg ccgcgctgaa acaggtgccg gcggccgagt
     1021 acaatccgtc ggagtgcatt cagaccaaca taatcggcgc cgagaacgtg gtctgggcca
     1081 gcctgtccaa ccatgtgaag caggtcgtgg ccctgtcgac cgacaaggcc tgcaacccgg
     1141 tcaacctgta cggcgcgacc aagctggcct cggacaagac cttcgtggcg gccaacaacc
     1201 tgtcgggcga catcggcacg cggttcgcgg tggtccgcta cggcaacgtg gtcggctcgc
     1261 gcggcagcgt ggcgccgctg ttccgccgcc tgctggcgca gggcgccacc gaactgccgg
     1321 tcaccgacgc ccgcatgacc cgcttctgga tcactctcaa ccagggcgtc gacttcgtgc
     1381 tgtcctcgct ggtgctgatg cgcggcggcg agatcttcgt gcccaagatc ccgtccatgt
     1441 cgatgcccga cctggtccag gccatggcgc cgggggccgg catgcggatc gtcggcatcc
     1501 ggccgggcga gaagctgcac gagatcatgg tcggagccga cgacgcgcgg cagaccgtcg
     1561 aactggccga ccgctacgtc atcgagcccg ccttcgtcga atacacgcgc aggccgttcg
     1621 gcccggccga cggcggcagg ccggtcgagg agggcttctc ctacgccagc gacaacaaca
     1681 gcgactggct gtcggccgag cggctgaggg ccatgctgga ggagacgggc gcgtgaccac
     1741 ggggttcctc ccctacggcc gccagaccat cgaggaggac gacatcgccg cggtggccgc
     1801 cgccctccgc gccgactacc tgaccaccgg cccggccgtg gaggcgttcg agacggcctt
     1861 tcgcgaggtg gtcggcgccg cgcacgccgt ggcctgcagc aacggcacgg ccgccctgca
     1921 tttggccatg ctggcggccg ggatcggacc gggcgacgtc tgcatcgttc ccgcgatcac
     1981 cttcctcgcc accgcgaact gcgccgccta cgtcggggcg gacgtggtgt tcgccgacgt
     2041 cgatccggac agcggcctga tgacgccgga gaccctcgcc gaggcgctgg accgggcccg
     2101 gggacggccg gtgcgggcgg tgctgcccgt gcatctgcgc ggcgatgtct gcgacctgcc
     2161 ggcgctggcg
//
LOCUS       DAORNX010000485         2166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37868_length_2166_cov_7.524870, whole genome shotgun sequence.
ACCESSION   DAORNX010000485 DAORNX010000000
VERSION     DAORNX010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37868_length_2166_cov_7.524870"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /gene="rpsT"
                     /locus_tag="PLO65_15590"
     CDS             complement(<1..263)
                     /gene="rpsT"
                     /locus_tag="PLO65_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRD29724.1"
                     /translation="MANNPGAKKAIRKIERRTEVNKARRSRVRTFLRKFDEALATGDA
                     AAAKAAFIVAQSELMRAVSKGVVHRNTGSRKVSRLNAQLKKLSA"
     gene            406..825
                     /locus_tag="PLO65_15595"
     CDS             406..825
                     /locus_tag="PLO65_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29725.1"
                     /translation="MDRRAFLVSTLALAGCGAASPRQVVICDPGLGAALMRARARYPQ
                     PGALVVSEARPQALLEMAEGERSALVVTRQSLIANRLQRLGHVRLEHRWQARIGKDAV
                     QLLVTRGGGPSQWRALKLARWLASDAGAAALGVPIGP"
     gene            complement(812..1591)
                     /locus_tag="PLO65_15600"
     CDS             complement(812..1591)
                     /locus_tag="PLO65_15600"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD29726.1"
                     /translation="MTYQTLIVETPAEGVTLIRLNRPEALNALNSQLLDELGQALDAA
                     AADEAVRCLVLTGSDRAFAAGADIKEMSDKSYAQMMALDFFTAGARKLEQFRKPVIAA
                     VSGYALGGGCELAMMCDFIIASETAKFGQPEINLGVSPGIGGTQRLTRFVGKAKAMDM
                     ILTARMMDAAEAERAGLVSRVVPVDKLLETALEAAGRIAGQSPLAVMMNKELVEAAYE
                     TTLATGVALERRLFHSLFAFEDQKEGMAAFVEKRKPDFKGR"
     gene            complement(1638..>2166)
                     /locus_tag="PLO65_15605"
     CDS             complement(1638..>2166)
                     /locus_tag="PLO65_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRD29727.1"
                     /translation="GRLGVAPAAIDRLRPWFADVTIGVVALMSRGASVTTGVERILAE
                     AAPTARRRAFETPADQIAFFADAPLADQLASFEDTLKQLDEDPAFYDRLISAWAEGDT
                     RAIEDIGLTPMKQVSLYLYDRLIVQRNRRWAETIAGRMAGSGETVIVVGVGHLVGPDS
                     VPALLRARGFTVDGP"
BASE COUNT          301 a          732 c          799 g          334 t
ORIGIN      
        1 gccgacagct tcttcagctg ggcgttcagg cgcgagacct tgcgcgagcc ggtgttccgg
       61 tggaccacgc ccttggacac ggcccgcatc agttccgact gggcgacgat gaaggcggcc
      121 ttggcggcgg cggcgtcgcc ggtggccagg gcttcgtcga acttgcgcag gaaggtgcgg
      181 acgcgcgaac ggcgggcctt gttcacttcc gtgcggcgct cgatcttgcg gatcgccttc
      241 ttggcgccgg gattattggc catgggcttg tagaacctca gaacggacgg gcgggcgcga
      301 acggccgcgc acgccggata aatcgaaggg cgggcaaata caggaaggcc cccagccggt
      361 caatgcgact ctggcggttc ggagggcgcc atgtagagtc cgggcatgga ccgccgcgca
      421 tttctcgtct cgaccctggc gttggccggc tgcggcgcgg cgtcgccccg tcaggtggtg
      481 atctgcgatc caggcctcgg cgcggccctg atgcgggcgc gggcgcgcta tccacaaccg
      541 ggcgccctgg tggtcagcga ggcccggccc caggcgctgc tcgagatggc cgagggcgag
      601 aggtcggcgc tggtggtcac ccgccagagc ctgatcgcca accgcctgca gcgactgggt
      661 catgtgcggc tcgaacatcg ctggcaggcg cggatcggca aggacgccgt gcagctgctg
      721 gtgacccggg gtggcgggcc gtcgcagtgg cgcgccctga agctcgccag gtggctggcg
      781 agcgacgcag gggcggcggc gctgggggtc cctatcggcc cttgaagtcg ggctttcgct
      841 tctcgacgaa ggccgccatg ccctccttct gatcctcgaa ggcgaacagg gagtggaaca
      901 gccgccgctc cagggccacc cccgtggcga gggtggtctc gtaggcggcc tcgaccagct
      961 ccttgttcat catcaccgcc agcggcgatt ggccggcgat cctgccggcg gcctccagcg
     1021 ccgtctcgag cagcttgtcg acgggaacga cacgggagac caggcccgcg cgctcggcct
     1081 cggcggcgtc catcatccgg gcggtcagga tcatgtccat ggccttggcc ttgccgacga
     1141 agcgggtcag ccgctgggtc ccgccgatgc cgggcgagac gcccaggttg atctccggct
     1201 ggccgaactt cgccgtctcc gacgcgatga tgaagtcgca catcatcgcc agctcgcagc
     1261 cgccgcccag ggcgtagccg ctgaccgcgg cgatcaccgg cttgcggaac tgctccagct
     1321 tgcgggcgcc ggcggtgaag aagtccagcg ccatcatctg ggcgtaggac ttgtccgaca
     1381 tctccttgat gtcggcgccg gcggcgaagg cgcggtccga accggtcagc accaggcagc
     1441 ggacggcctc gtccgccgca gcggcgtcga gggcctggcc cagctcgtcc agcagctggc
     1501 tgttgagggc gttgagggcc tcggggcgat tgagacggat gagggtcacg ccctcggccg
     1561 gagtctcgac gatcagggtc tggtaggtca tgggcggggt tgtgcccgcc gtcagcgccc
     1621 ggctcaagcc tctttgctca ggggccgtcg accgtgaagc cgcgcgcgcg cagcagggcc
     1681 ggcacgctgt cggggccgac cagatggccg acgccgacga cgatcacggt ctcgccggac
     1741 ccggccatgc gcccggcgat ggtctcggcc cagcggcggt tccgctgcac gatcagccgg
     1801 tcgtagagat acagcgacac ctgtttcatc ggcgtcagcc cgatatcctc gatcgcgcgg
     1861 gtgtccccct cggcccaggc gctgatcagc cggtcgtaga aggccgggtc ctcgtcgagt
     1921 tgcttgagcg tgtcctcgaa cgaggcgagc tggtcggcca gcggcgcgtc ggcgaagaag
     1981 gcgatctggt ccgccggagt ctcgaaggcg cgtcgccggg cggtcggggc ggcctcggcc
     2041 aggatgcgct cgacgcccgt ggtcacgctg gcgccccggc tcatcagtgc caccacgccg
     2101 atggtcacgt cggcgaacca gggtctcagc cggtcgatgg cggccggcgc cacgcccagc
     2161 cggccg
//
LOCUS       DAORNX010000486         2163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_37929_length_2163_cov_5.589184, whole genome shotgun sequence.
ACCESSION   DAORNX010000486 DAORNX010000000
VERSION     DAORNX010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37929_length_2163_cov_5.589184"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2163
                     /locus_tag="PLO65_15610"
     CDS             <1..>2163
                     /locus_tag="PLO65_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD29728.1"
                     /translation="AAADAAPARPVRLAPGQWPQAVSDVAADPDVRFGALPNGMRYAI
                     IHNATPPGQASLRLRFDAGSLMETDAQQGLAHFLEHMAFNGSKGIAEGEMVKMLERRG
                     LAFGADTNASTNFDETTYKLDLPQTDDETVDLSLKVLRETASELLIAADAVDRERGVV
                     LSEERTSDSPGLRVFKARMAFLLRGQLPPKRYPIGKVEVLRTAPREQLVDFYSRYYRP
                     ERAVLVAVGDFDVDAMEARIKATFGGWTNTAPAGSDPDLGVVEKRGLETQIVVEPGAS
                     TTLQIGWTSAPDLRPDSAAKRRQDWIQQLGFAVLNRRLEAIARGAEPPFIAAGGFSGN
                     QVDAAKVTTLAVTARPGEWRAALGAAEREQRRLTQYGVRQDELDREIEDYRQWLKSRL
                     ASAATRRTPQLASEMIGTVGDEDVITSPAQDLALFEAAVKDLKAETVSAAAKAAFAGS
                     GPLVFIATPTPIEGNEQAVAEAWNASREIAVAAPTGPRQVTWPYEDFGAPGKVVEQTD
                     LVDLDTTFVRFENGVRLTVKPTRFRDDQVLVKVRIGSGMTALPADQQSLSWAGSALIE
                     GGLAKISSEDMERVLVSKVYGARFGVEDDAFALSGATRPDDLATQLQVLAAFASDPGW
                     RPEALQRMRTYAATLSDQYEATDSGVFNRDLAGLLHGGDRRWTWPTKADIAGVNAEGL
                     KHSFGDALAGGPVEVLIVGDITVEKAIAAVAATFGALP"
BASE COUNT          335 a          777 c          776 g          275 t
ORIGIN      
        1 ggcggcggcc gacgccgctc ctgccaggcc ggtcaggctg gcgcccgggc aatggcccca
       61 ggccgtcagc gacgtggcgg ccgacccgga cgtgcgcttc ggggcgctgc ccaacggcat
      121 gcgctacgcc atcatccaca acgccacccc gccgggccag gcgtcgctgc gtctgcggtt
      181 cgacgccggc tcgctgatgg agaccgacgc ccagcagggg ctggcccact tcctcgagca
      241 catggcgttc aacggcagca agggcatcgc cgagggcgag atggtcaaga tgctcgagcg
      301 gcggggcctg gccttcggcg ccgacaccaa cgcctcgacc aatttcgacg agacgaccta
      361 caagctcgac ctgccgcaga ccgacgacga gaccgtcgac ctgtcgctga aagtgctgcg
      421 cgagacggcc agcgagctgc tgatcgccgc cgacgcggtc gaccgcgagc gcggggtggt
      481 gctgtcggag gagcgaacca gcgacagccc cggcctgcgg gtgttcaagg cccggatggc
      541 cttcctgctg cgcgggcagc tgccgccgaa acgctatccg atcggcaagg tcgaggtgct
      601 gcgcaccgcg ccgcgcgaac agctggtcga cttctacagc cgctactatc gccccgagcg
      661 ggcggtcctg gtggcggtcg gagacttcga cgtcgatgcc atggaagcgc ggatcaaggc
      721 gaccttcggc ggctggacca acactgcgcc ggccgggtcc gaccctgacc tgggcgtggt
      781 ggagaagcgc ggcctggaaa cgcagatcgt cgtggagccg ggcgcctcga cgacgctgca
      841 gatcggctgg acctcggccc cggacctgcg gcccgacagc gccgccaagc ggcggcagga
      901 ctggatccag cagctcggct tcgccgtgct caatcgccgg ctggaggcca tcgcccgcgg
      961 cgccgagccg ccgttcatcg ccgccggcgg cttttccggc aaccaggtcg acgcggccaa
     1021 ggtgaccacc ctcgccgtca ccgcccggcc gggcgaatgg cgggcggccc tgggcgcggc
     1081 cgagcgcgaa cagcggcggc tgacccagta cggcgtgcgg caggacgagc tggaccgcga
     1141 gatcgaggac taccgccagt ggctcaagtc acgcctggcc tcggccgcca cgcggcgcac
     1201 gccgcagttg gccagcgaga tgatcggcac ggtcggcgac gaggacgtca tcaccagccc
     1261 ggcccaggac ctcgcgctgt tcgaggcggc ggtgaaggac ctcaaggccg agaccgtttc
     1321 ggccgccgcg aaggccgcgt tcgccggatc cggaccgctg gtgttcatcg ccacgccgac
     1381 gccgatcgag ggcaacgaac aggcggtcgc cgaggcctgg aacgcctcgc gcgagatcgc
     1441 cgtggccgcg ccgaccggac cgcgccaggt cacctggccc tacgaggact tcggcgcgcc
     1501 cggcaaggtc gtcgagcaga ccgacttggt cgacctggac accaccttcg tgcgcttcga
     1561 gaatggcgtg cggctgacgg tcaagccgac ccggttccgc gacgaccagg tgctggtgaa
     1621 ggtgcgcatt ggcagcggca tgaccgcctt gccggcggac cagcagtcac tgtcctgggc
     1681 cggctcggcc ctgatcgagg gcggcctggc gaagatcagt tccgaggaca tggagcgggt
     1741 gctggtgtcg aaggtctacg gcgcccggtt cggcgtcgag gacgacgcct tcgccctgtc
     1801 cggcgcgacc cggcccgacg acctggccac ccagttgcag gtgctggccg ccttcgccag
     1861 cgatccgggc tggcggcccg aggcgctgca gcggatgcgg acctacgcgg cgactctgag
     1921 cgaccagtac gaagccaccg acagcggcgt gttcaaccgc gacctggcgg ggctgctgca
     1981 cggcggcgac cgccgctgga cctggccgac caaggccgac atcgccggcg tcaacgccga
     2041 aggcctcaag cacagcttcg gcgacgccct ggccggcggg ccggtcgagg tgctgatcgt
     2101 cggcgacatt accgtcgaga aggccatcgc cgccgtcgcc gccaccttcg gcgccctgcc
     2161 cca
//
LOCUS       DAORNX010000487         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38014_length_2159_cov_4.887833, whole genome shotgun sequence.
ACCESSION   DAORNX010000487 DAORNX010000000
VERSION     DAORNX010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38014_length_2159_cov_4.887833"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>267)
                     /locus_tag="PLO65_15615"
     CDS             complement(<1..>267)
                     /locus_tag="PLO65_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29729.1"
                     /translation="AVFDNIPLTALALKQGGYDWGVLAFAVGFGGSMMWFGSSAGVAL
                     SGLFPQVRSVGAWLRHGWYVPVAYLAGFAAMVSLAGWHPTPTRAE"
     assembly_gap    268..293
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<294..1252)
                     /locus_tag="PLO65_15620"
     CDS             complement(<294..1252)
                     /locus_tag="PLO65_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29730.1"
                     /translation="MNVLLEGLAALPGLLAATPGEFWIFGLTLLGVALFHNRTLTVAL
                     AGLTATVLYKLAFTGFAHGPGLEGLLAHARHESVILGNLMLLLLGFALLANQFERSNI
                     PEAMPRVLPDDWKGGLALLAMVFVLSAFLDNIAAAIIGGVVARHVYHDKVSVGFLASI
                     VAASNAGGAGSVIGDTTTTMMWISGVSPLAVLHAFIASVVAFAVFGLLGAWQQHRHFP
                     IQAHIAADAPKVDPARAVIVGLILATLLAVNFAGNAWYPEHQDAAPWLGLGLWAAVLV
                     TAVVRAPQWRVLPGAARGALFLVSLVATASLMPVERLPDASWPT"
     gene            complement(1339..1623)
                     /locus_tag="PLO65_15625"
     CDS             complement(1339..1623)
                     /locus_tag="PLO65_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HRD29731.1"
                     /translation="MRLAVRLTPRGGRDAIDGWASGADGRPYLKARVSAPPVEGEANA
                     ALTVLLAKALGVSKSSVRIAAGQTGRLKSVEIEGLSEADATAVFGPPPKG"
     gene            complement(1628..1939)
                     /locus_tag="PLO65_15630"
     CDS             complement(1628..1939)
                     /locus_tag="PLO65_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRD29732.1"
                     /translation="MAAILGFVFFIINSLLGLLILALVITAVMSWLVAFDVINLRNNA
                     VRQIYYALERITEPLLAPIRRFVPPLGGVDLSFIILFLIVQGVRGYLLPGLYAALMGL
                     A"
     gene            2084..2159
                     /locus_tag="PLO65_15635"
     tRNA            2084..2159
                     /locus_tag="PLO65_15635"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2117..2119,aa:Ala,seq:cgc)
BASE COUNT          363 a          739 c          773 g          258 t
ORIGIN      
        1 ctcggcccgg gtcggcgtcg gatgccagcc ggccagcgag accatggccg cgaatccggc
       61 gagataggcg accggcacat accagccgtg gcgcagccag gcgccgaccg accggacctg
      121 cgggaacagg cccgacagag cgaccccggc cgaggagccg aaccacatca tcgagccgcc
      181 gaagccgacc gcgaaggcca gcacgcccca gtcatagccg ccctgcttca gggccagggc
      241 cgtcagcggg atgttgtcga acaccgcnnn nnnnnnnnnn nnnnnnnnnn nnngtcggcc
      301 aggaggcgtc gggcaggcgc tcgaccggca tcagcgaggc ggtggccacc agcgacacca
      361 ggaacagcgc gccccgggcg gcccccggca gcacgcgcca ttgcggggcc cgcacaacgg
      421 cggtgaccag caccgccgcc cacagtccca gccccagcca gggcgccgcg tcctggtgct
      481 cggggtacca ggcgttgccg gcgaaattga ccgccagcag ggtggccaga atcaggccga
      541 cgatgaccgc ccgggccgga tcgactttcg gcgcgtcggc ggcgatatgg gcctggatcg
      601 ggaagtgccg gtgctgttgc caggcgccga gcaggccgaa caccgcgaag gccaccaccg
      661 aggcgatgaa ggcgtgcagc acggccagcg gcgacacgcc gctgatccac atcatcgtgg
      721 tggtggtgtc gccgatcacg ctgccggccc cgccggcgtt ggaggccgcc acgatgctgg
      781 ccaggaagcc gacgctgacc ttgtcatgat agacatgccg ggccaccacc ccgccgatga
      841 tcgccgcggc gatgttgtcg aggaaggccg acagcacgaa caccatggcc agcagggcca
      901 ggccgccctt ccagtcgtcg ggcaggacgc gcggcatggc ctccgggatg ttgctgcgct
      961 cgaactggtt ggccagcagg gcgaagccca gcagcagcag catcaggttg ccgaggatca
     1021 ccgattcgtg ccgggcgtgg gccagcaggc cctccagccc cggtccgtgg gcgaagccgg
     1081 tgaaggccag cttgtacagc acggtcgccg tcaacccggc cagggccacg gtcagggtgc
     1141 ggttgtggaa cagggccacg cccagcaggg tcaggccgaa gatccagaat tcccccggcg
     1201 tcgcggcaag cagacccggc agggccgcga ggccctccag caagacgttc atggcgcggc
     1261 gatccccccg gtgcggcgtt ttgcggggac actgtgctga gccggcgagg cagaatcaac
     1321 cccggcgccg cgaccgccct atcccttcgg gggcggcccg aagacggctg tggcgtcggc
     1381 ctcggacagc ccctcgatct cgaccgactt gagccggccg gtctggccgg cggcgatgcg
     1441 gacggaggat ttgctgacgc ccagcgcctt cgccagcagc acggtcaggg cggcgttggc
     1501 ctcgccctcg accggcgggg cgctgacgcg ggccttgaga taggggcggc cgtccgcgcc
     1561 gctggcccag ccgtcgatgg cgtcccggcc gccgcgcggc gtcaggcgga cggccagtcg
     1621 catggcgtca ggccaggccc atcagcgccg cgtagaggcc gggcagcaga tagccgcgga
     1681 cgccctggac gatcaggaac aggatgatga agctcaggtc gaccccgccg agcggcggca
     1741 cgaaccggcg gatcggcgcc agcagcggct cggtgatccg ctccagggcg tagtagatct
     1801 ggcgcacggc gttgttgcgc aggttgatca cgtcgaaggc gaccagccag gacatcaccg
     1861 cggtgatgac cagggccagg atcaacaggc ccagcaggct gttgatgatg aagaagacga
     1921 agccgaggat ggcagccatg acgcagggtt ccggtggtct gtgagcctgc ttctagtcgc
     1981 gccgcccagt cgcagcaagg cgcgcgggcc ccgcctgcgg ccgccagcct tgacaggcaa
     2041 ggcgacccgg ccatatgtgc gccgcttccc tcgtcctggg gagggggccg tagctcagat
     2101 ggtagagcgc ctcgttcgca atgaggaggt caggggttcg attcccctcg gctccacca
//
LOCUS       DAORNX010000488         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38016_length_2159_cov_4.418726, whole genome shotgun sequence.
ACCESSION   DAORNX010000488 DAORNX010000000
VERSION     DAORNX010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38016_length_2159_cov_4.418726"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            487..912
                     /locus_tag="PLO65_15640"
     CDS             487..912
                     /locus_tag="PLO65_15640"
                     /inference="COORDINATES: protein motif:HMM:NF019023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HRD29733.1"
                     /translation="MCDARSQLAEWFKDLWGEPLPEDGDTDLFDRFGIEGDDAGEFIE
                     SFVAHFQVDAQNYRWYFHHQDEGTNFGALLFTPIYRRVERLPITPNLLIAAIETQQWP
                     IKYPPHHIPPVRWDIRVNQLSFAVAIIVLGIWLWQRFVP"
     gene            complement(955..>2159)
                     /locus_tag="PLO65_15645"
     CDS             complement(955..>2159)
                     /locus_tag="PLO65_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRD29734.1"
                     /translation="VPARAWTTDLRKLVVAGLAAGDYAGAYGAAANTGLTVGPDAAEA
                     EFYAGWIALSKLRNPAAAAQHFARLAAAGTSPITQGRALYWQGRAAEALGDKAAAQGF
                     YQQGAQHITVFYGQLAAERAGLRTLTLPRDPVITQADRARFEGRELVRALRLLYETGN
                     RDTFKVFALHVDDTLPTAAECALLVDLARSYGDPDTAMRAVRTAAQRGMILPERGYPL
                     LAAPSVPGGAEPAFVFSITRQESNFDSRARSAPGARGLMQLMPATGAMTARALGESYS
                     VDRLFDADFNMRLGARYLGDMINTFSGSYIMAAAAYNAGPGRPPQWAALCGDPRGATT
                     DPLDFIECIPFVETRNYVMRTLETMQVYRARLNGGSAPLTLSSDLKRGGWGGYTPTAA
                     PAAAPGTR"
BASE COUNT          317 a          712 c          767 g          353 t
ORIGIN      
        1 gtctgtgacc ggctggtggt gctgtcgcgc ggtcaggtgg ccgccgacgg ccgtcccgcc
       61 gaggccctcg cgccagacat cctgcgggtg gcgttcgggc tcgacggcgg cctggtggag
      121 acccccgccg ggccggtggt cgccgcgcgc cgcgctggtt cctagggttc acgccgccca
      181 cattccgtca tggccccgac aagccgggcc atgacggtgg aatagacgga aaggtagggc
      241 cacaaccggc cctgatctca tggcgagagg ccggcacgct ggcccgcaan nnnnnnnnnc
      301 agtctcccca gcctgctcaa gggcgcttcg cgtcgcgcag cgacaggccc gcaaggcctg
      361 cccttgacca ggctggggag actggcacgc tggcctgcaa gagatcgacg gcgaagtgtc
      421 gactgagggt ggtgagcgga cgttgaccca ctgtccgatt gcgtgacttg gcggtatcag
      481 ttgcgaatgt gtgacgcgcg ttcccagcta gcggaatggt tcaaggacct ctggggggag
      541 ccccttccgg aagacggcga caccgacctg tttgatcggt tcggtataga gggtgatgac
      601 gccggcgagt ttatcgaaag cttcgtggcc cactttcaag tcgatgccca gaactaccgc
      661 tggtattttc atcatcagga tgaaggaacg aacttcggtg cgctcctttt cacgccgatc
      721 tatcggcgcg tggagcgttt accgatcacg ccgaacctcc tgatcgctgc aatcgagact
      781 cagcagtggc cgatcaaata cccgccacat cacatcccgc ccgtgcgctg ggatatcagg
      841 gtgaaccagc tatcttttgc cgtcgcgatt atcgtgttgg gcatatggct ttggcagcgt
      901 tttgtcccct aagggtcgaa agccgctgtt cgccttccgc tcgaagcccg ccccctaccg
      961 cgttcccggc gccgccgccg gcgccgcggt cggggtataa ccgccccagc cgccgcgctt
     1021 gaggtcgctc gacagggtca ggggggccga gccgccgttc agccgggccc ggtagacctg
     1081 catcgtctcc agcgtccgca tgacgtagtt gcgcgtctcg acaaacggga tgcactcgat
     1141 gaagtccagc ggatcggtcg tcgccccgcg cgggtcgccg cacagcgccg cccactgcgg
     1201 cggccggccg ggcccggcgt tataggcggc cgcggccatg atgtacgacc cgctgaaggt
     1261 gttgatcatg tcgcccaggt agcgggcgcc cagccgcatg ttgaaatcgg cgtcgaacag
     1321 ccggtcgacc gagtagctct cgccgagcgc gcgggcggtc atggccccgg tcgccggcat
     1381 cagctgcatc aggccgcggg ccccgggggc cgagcgggcg cgggagtcga agttgctttc
     1441 ctggcgggtg atcgagaaca cgaaggccgg ctcggccccg ccgggcacgc tgggcgccgc
     1501 tagcagggga tagccgcgtt cgggcaggat cataccgcgc tgggccgcgg tgcggacggc
     1561 ccgcatggcg gtgtcgggat cgccgtagct gcgggccaga tcgaccagca gggcgcattc
     1621 ggcggccgtc ggcagggtgt cgtcgacatg cagcgcgaac accttgaagg tgtcgcggtt
     1681 gccggtctcg tagagcagcc gcagggcgcg gaccagttcg cggccctcga agcgggcgcg
     1741 gtcggcctgg gtgatcaccg ggtccctggg cagggtcagg gtcctcagcc cggcccgttc
     1801 ggcggccagc tggccgtaga acacggtgat gtgctgcgcg ccctgttgat agaagccctg
     1861 ggccgcggcc ttgtcgccga gcgcctcggc ggcgcggccc tgccagtaga gggcccgtcc
     1921 ctgggtgatc ggcgatgttc cggcggcggc cagtctggcg aaatgctgcg cggcggcggc
     1981 cgggttcctc agcttcgaaa gcgcgatcca gccggcgtag aattccgcct cggccgcatc
     2041 gggaccgacg gtcagcccgg tgttcgccgc cgcgccgtag gcgccggcgt agtcaccggc
     2101 cgccagcccg gccacgacca gcttgcgcag atcggtggtc caggcccggg cgggcacgg
//
LOCUS       DAORNX010000489         2151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38187_length_2151_cov_7.262405, whole genome shotgun sequence.
ACCESSION   DAORNX010000489 DAORNX010000000
VERSION     DAORNX010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38187_length_2151_cov_7.262405"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="PLO65_15650"
     CDS             <1..2001
                     /locus_tag="PLO65_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD29735.1"
                     /translation="APAATVAGEAIARRKATSPDAVSLLLGEPGVGVQAGGGTTALPV
                     VRGMADDRMLVTVDGAEATAFCPNHMNPPGSYVLAGGVASIALSPTLSPVSAGGDNIG
                     GVIAITTRGPVFAATGESPRREGEASFAYRSVVDGGSASARASIAGERLSLGVESAWA
                     RGENYRSGYGRDVLSTEYESYEGAVTLAARTGQGVVSLRAGRQFTPYQAFPNQRMDMT
                     ENASRFADLRYDGAYAWGDVTARASWRDVDHEMNFLPDKGGDMPMLTHGVDLGLTLAV
                     TLPAGQDASLALGLDVRRTDLDDWWPPVAGSMMMGPNPYLNINNGSRDRTGLWAEWAG
                     PLGGAWSGVAGLRLERVETNAGDVQPYSWTGMMNAADIAAATAFNARDRKRTDDVADV
                     TLLARWDPSAAVSLELGYARKTRTPNLYERYAWGMGQMSAAMTSLAGDANAYVGDMDL
                     RPETANNLGATLRLSAPAAGRQLTVSLFHAEIEDFVDADPIGVFGPGGSFARLRFANH
                     DARMQGVEVSGRTDLWTIGGFAPAVLTGSVSWVQGENRDTGDDLYHMAPLTTRVSLEQ
                     PLGAWTAVAELELVAEKDRINALRNEPVTDAYALFNLRGVWRPTERLRFDMALENLFD
                     EGYDLPLGGVSFGDFKAGGMVPPIAALPGPGRSFNLGLTVGF"
BASE COUNT          298 a          768 c          774 g          311 t
ORIGIN      
        1 gcccccgcgg ccaccgtcgc cggcgaggcg atcgcgcggc gaaaagccac aagtccagac
       61 gccgtctccc tgctgctggg cgaaccgggc gtcggcgtcc aggccggcgg cggcacgacg
      121 gccctgccgg tcgtgcgcgg catggccgac gatcgcatgc tggtcaccgt cgacggggcc
      181 gaggccacgg ccttctgtcc caaccacatg aacccgccgg gctcctatgt tctggcgggc
      241 ggcgtggcct cgatcgccct gtctccgacc ctgtcgccgg tcagcgccgg gggcgacaac
      301 atcggcgggg tgatcgccat cactacgcga ggccccgtgt tcgccgcgac cggcgaaagc
      361 ccgcgcaggg aaggggaggc gagctttgcc taccgcagcg tggtcgacgg cgggtccgcc
      421 tccgcgcggg cgtccatcgc gggcgagcgg ctgagcctgg gcgtcgagtc ggcctgggcc
      481 cgtggcgaga actatcgctc cggctacggc cgcgatgtgc tgtcgaccga gtacgagtcc
      541 tatgaggggg ccgtgacgct cgcggcccgg accggccagg gcgtggtcag cctgcgggcc
      601 ggccgtcagt tcacgcccta ccaggccttc cccaaccagc gcatggacat gacggagaac
      661 gccagccgtt tcgccgacct tcgctatgac ggcgcctacg cctggggcga tgtcacggcc
      721 cgggcttcgt ggcgcgacgt cgaccacgaa atgaacttcc tgcctgacaa gggcggggac
      781 atgccgatgc tgacccatgg cgtggacctg ggcctgaccc tggccgtgac gctcccggcc
      841 gggcaggacg ccagcctggc gctgggcctg gatgtccgcc ggaccgacct ggacgactgg
      901 tggccgccgg ttgccggcag catgatgatg ggccccaacc cctatctcaa catcaacaac
      961 ggctcgcgcg accgcacggg tctgtgggcc gagtgggccg gaccgctggg gggcgcctgg
     1021 agcggcgtcg ccggcctgcg gctggaacgg gtcgagacca atgccggcga cgtccagccc
     1081 tacagctgga ccggcatgat gaacgccgcc gacatcgccg ccgccacggc gttcaacgcc
     1141 cgtgaccgca agcggacgga cgatgtcgcc gacgtgaccc tgctggcccg ctgggatccg
     1201 tcggcggccg tgtcgctcga gctgggctat gcgcgcaaga cccgcacgcc gaatctctac
     1261 gaacgctacg cctggggcat gggtcagatg tcggcggcca tgacctccct cgccggggac
     1321 gccaacgcct atgtcggcga catggacctc cggccggaga cggccaacaa cctcggggcc
     1381 accctgcgcc tgtcggcccc cgcggccggc cggcaactga ccgtcagcct gtttcacgcc
     1441 gagatcgagg actttgtcga tgccgacccg atcggagtgt tcggccccgg cggcagcttc
     1501 gcgcggctgc ggttcgccaa ccacgacgcg cggatgcagg gcgtggaggt cagcggccgc
     1561 accgacctgt ggaccatcgg cggcttcgcg ccggcggtgc tgaccggttc ggtctcgtgg
     1621 gtgcagggcg agaaccgcga caccggcgac gatctctacc acatggcccc gctgaccacg
     1681 cgggtcagcc tggagcagcc gctgggcgcc tggacggcgg tcgccgagct tgagcttgtc
     1741 gccgagaagg accggatcaa cgccctgcgc aacgagccgg tcaccgacgc ctacgccctg
     1801 ttcaacctgc ggggcgtctg gcggccgacc gagcggctgc ggttcgacat ggcgctcgag
     1861 aacctgttcg acgagggcta tgacctgccg ctcgggggcg tgtcgttcgg cgacttcaag
     1921 gccggcggca tggtcccgcc aatcgccgcc ctgcccggac cggggcggtc gttcaacctc
     1981 ggcctgacgg tcgggttctg accatggcgt cgcgcggcgt cgggattgcg acggtggtct
     2041 cgatcggcct gcatctgctg gtcatcgcgg ccctgtggcg gtcgccggcg cccgtccatc
     2101 gcggaccggg gccggcgctc gacgtggttc tggcgccgac catggtcgag c
//
LOCUS       DAORNX010000490         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38216_length_2150_cov_7.902625, whole genome shotgun sequence.
ACCESSION   DAORNX010000490 DAORNX010000000
VERSION     DAORNX010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38216_length_2150_cov_7.902625"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2150
                     /locus_tag="PLO65_15655"
     CDS             <1..>2150
                     /locus_tag="PLO65_15655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRD29736.1"
                     /translation="LIVPPDRLDAAFETRAAALAGELRGRVWLAASRLYGSRDAKRLH
                     RLSALAQRIGAPMVATGDVLYHAPERRPLQDVLTCIREGCTIGEAGWRLEANAERHIK
                     SPAEMARLFAAWPEAVERSVEIAERISFDLGALREEYPDEPVPPGKTAMQHLTKLTWD
                     GAKRRYPGGVPEKVRANLRTELRLIRKMNYPNYFLTVHDIVAEARRLGILCQGRGSAA
                     NSSVCFCLHVTAIDPMEHRLLFTRFISENRGEPPDIDVDFEHERREEVMQYVYRRYGR
                     HKAAICATVIHYRPRSAIRDVGKALGLTEDITAALAGTVWGSWGGEIARDHIRQVGLD
                     PDNPEIVRATTLAAELLNFPRHLSQHVGGFVLTRRRLDETVPIGNAAMKDRTFIAWDK
                     DDIDALGLMKVDILALGMLTALQRGLTLLRRDHGQPLRDLADIPIEEKGVYDMLSKAD
                     SVGVFQVESRAQMSMLPRLKPATFYDLVIEVAIVRPGPIQGKMVHPYLRRRQGLEAPE
                     WPSPSPEHGPADELKQVLGQTLGVPLFQEQAMSLAIEAAKFTPGEADGLRRAMATFRN
                     LGDPAEYRERFVGGMVSRGYDPEFAARCFSQIEGFGSYGFPESHAASFAKLVYVSAWM
                     KWLWPDVFCAALLNSQPMGFYQPAQLVRDAREHGVEVRPVDALRSDWDCTLEPAADPG
                     ARLKAVRLGLRQVKGLREDEIERLMAHRAGATSL"
BASE COUNT          323 a          757 c          763 g          307 t
ORIGIN      
        1 gcctgatcgt tccgccggac cggctcgacg cggcgttcga gacccgggcg gcggccctgg
       61 ccggcgagct gcgcggacgg gtctggctgg ccgcctcgcg cctctatggc tcgcgggacg
      121 ccaagcggtt gcaccgcctg tcggccctgg cccagcggat cggcgcgccg atggtcgcca
      181 ccggcgatgt gctctaccac gcgcccgaac ggcgaccgct gcaggacgtg ctgacctgca
      241 tccgcgaggg ctgcacgatc ggcgaggccg gttggcggct cgaggccaac gccgagcggc
      301 acatcaagtc ccccgccgag atggcccgcc tgttcgcggc ctggcccgag gcggtggagc
      361 gctcggtcga gatcgccgag cggatcagct tcgaccttgg cgccctgcgc gaggaatatc
      421 cggacgagcc cgtgccgccg ggcaagacgg ccatgcagca tctgacgaag ctgacctggg
      481 acggggcgaa gcggcgctat cccggtggcg tgcccgaaaa ggtcagggcc aacctgcgga
      541 ccgagctgcg gctgatccgc aagatgaact atcccaacta cttcctgacc gtgcacgaca
      601 tcgtcgccga ggcgcggcgg ctgggcatcc tgtgccaggg acgggggtcg gcggccaact
      661 cctcggtctg tttctgcctg catgtgacgg ccatcgaccc gatggagcac cgcctgctgt
      721 tcacccgctt catctccgag aaccgcggcg agccgccgga catcgacgtc gacttcgagc
      781 acgagcggcg cgaggaggtg atgcagtacg tctaccgccg ctacgggcgg cacaaggcgg
      841 ccatctgcgc caccgtgatc cattatcggc cgcgcagcgc gatccgcgac gtcggcaagg
      901 ccctgggcct gaccgaggac atcaccgccg ccctggcggg cacggtctgg ggcagctggg
      961 gcggcgagat tgcccgggat cacatccgcc aggtcggact cgaccccgac aatcccgaga
     1021 tcgtccgggc caccaccctg gcggccgagc tgctgaactt tccgcgccac ctgtcgcagc
     1081 atgtcggcgg cttcgtgctg acccggcggc ggctggacga gaccgtgccg atcggcaacg
     1141 cggcgatgaa ggaccgcacc ttcatcgcct gggacaagga cgacatcgac gccctggggc
     1201 tgatgaaggt cgacatcctg gcgctgggca tgctgaccgc cctgcagcgc ggcctgaccc
     1261 tgctgcgccg cgaccacggc cagccgctgc gcgacctggc cgacatcccg atcgaggaga
     1321 agggcgtcta cgacatgctg tcgaaggccg actcggtggg cgtgttccag gtcgagagcc
     1381 gggcccagat gtcgatgctg ccccggctga agccggcgac cttctacgac ctggtcatcg
     1441 aggtggcgat cgtccggccc gggccgatcc agggcaagat ggtccacccc tacctgcgcc
     1501 gccgccaggg gctggaggcg ccggaatggc cttcgccctc gcccgagcac gggccagccg
     1561 acgagctgaa gcaggtgctg ggccagaccc tcggcgtgcc gctgttccag gaacaggcca
     1621 tgagtctggc catcgaggcg gcgaagttca ccccgggcga ggcggatggc ctgcgccggg
     1681 cgatggccac gttcaggaac ctcggcgacc cggccgaata ccgcgagcgg ttcgtcggcg
     1741 gcatggtctc gcgcggctac gatccggagt tcgcggcccg ctgcttcagc cagatcgagg
     1801 gtttcggctc ctacggcttc ccggaaagcc acgccgccag cttcgccaag ctggtctatg
     1861 tctcggcctg gatgaaatgg ctctggccgg acgtgttctg cgcggccctg ctcaattccc
     1921 agccgatggg cttctaccag cccgcccagc tggtgcgcga cgcccgcgag cacggggtcg
     1981 aggtgcggcc cgtcgacgcc ctgcgcagcg actgggactg caccctggag ccggcggccg
     2041 atccgggcgc gcgcctcaag gccgtgcggc tcgggctgcg ccaggtgaag gggctgcgcg
     2101 aggacgagat cgagcggctg atggcccatc gcgccggggc gaccagcctg
//
LOCUS       DAORNX010000491         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38324_length_2145_cov_7.224402, whole genome shotgun sequence.
ACCESSION   DAORNX010000491 DAORNX010000000
VERSION     DAORNX010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38324_length_2145_cov_7.224402"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLO65_15660"
     CDS             complement(<1..396)
                     /locus_tag="PLO65_15660"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRD29737.1"
                     /translation="MTQSVLGRLRRVWLDVHLWIGVGLFVALVPIGLSGAFLVWHDPI
                     DAALHPQRFAVARNDTTLAASAYLDAARKAFGDRAAVTQLRLAEEPGDPVVVSARVKG
                     PPPAPGQRPATVTAWLDPATARVLDMADTR"
     gene            525..986
                     /locus_tag="PLO65_15665"
     CDS             525..986
                     /locus_tag="PLO65_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD29738.1"
                     /translation="MSFAIEAVGHVRGGRAEAIDDDWGPSRARIELDPARFGPEVLFG
                     LADFSHAEIVFVFDRVEEAKINTSARRPRNNPDWPLVGIFAQRGKARPNRLGVTVCRI
                     LAVNGLTIEVEGLDAIDGTPVVDIKPVLRGFLPRGEVREPAWAAELMEGYW"
     gene            983..>2145
                     /locus_tag="PLO65_15670"
     CDS             983..>2145
                     /locus_tag="PLO65_15670"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD29739.1"
                     /translation="MKRPLEGVRVLTIEHYGAGPYATLLMAELGAEVIKVEAPSMGGD
                     ASRPTGPFGLGDNDSQYFQTFSRGKKSAAIELKTPEGRTDFERLVADAEAVVNNLRGD
                     QPAKLGLTYAALKAINPAIVCGHLSAYGRNNSREAWPGYDYPMQAECGLMSLTGEPDG
                     PPARMGVSIIDFMSGATFAFGVVSAILSARATGEGCDVDVSLFDVALHQLSYPAVWAM
                     NERHPTVRMPNGAHPSLAPVQRVKTADGYGQLMCMNEKFWAEFCDTTGRVDLAADPRF
                     ATLADRARNLPALTQALDALFATRPTAHWSALFAGRVPFGPVNGLFEALDNPFVAEVA
                     MRDVVDHPDRPQGLRMLAMPVKIDGARMPGFSREANAGEGLDDIAGDALAGEIA"
BASE COUNT          334 a          738 c          777 g          296 t
ORIGIN      
        1 gcgcgtgtcg gccatgtcga gcaccctggc cgtcgccggg tcgagccagg cggtgacggt
       61 ggcggggcgc tgtccgggag ccggcggcgg gcccttgacc cgcgcgctga cgaccaccgg
      121 atcgccgggc tcctcggcca ggcgcagctg ggtcacggcg gcgcggtcgc cgaaggcctt
      181 gcgcgcggcg tcgagatagg cggaggccgc cagggtcgtg tcgttgcgcg cgacggcgaa
      241 ccgctgcggg tgaagcgccg cgtcgatcgg atcgtgccag accaggaaag cccccgacag
      301 gccaatcggg accagggcga cgaacaggcc gaccccgatc cacagatgca cgtcgagcca
      361 gacgcggcgc aggcggccga ggacggactg ggtcaaggcg gagatcccca caggatgaca
      421 gccccggcgt taatgcgaat gtttctcaaa tgcaagtcgg ggcgacttga ccggcctgtc
      481 cggcgggcct agcccgacgc gactgttcgg aaggggaggc gtccatgtcg ttcgcgatcg
      541 aggccgtggg ccatgtgcgc ggcggacggg ccgaggcgat cgacgacgac tggggtccca
      601 gccgggcgcg gatcgagctc gatccggcgc gcttcgggcc cgaggtcctg ttcggcctgg
      661 ccgacttctc ccacgccgag atcgtcttcg tgttcgaccg ggtcgaggag gcgaagatca
      721 acaccagcgc gcggcggccc cgcaacaatc ccgactggcc cctggtcggc atcttcgccc
      781 agcgtggcaa ggcgcggccg aaccggttgg gagtcaccgt ctgccgcatc ctcgcggtca
      841 acggcctgac catcgaggtc gagggtctgg acgcgatcga cggcacgccg gtggtcgaca
      901 tcaagccggt gctcaggggg tttctgccgc gcggcgaggt gcgggaaccc gcctgggcgg
      961 ccgagttgat ggaggggtac tggtgaagcg accgctcgag ggcgtccgcg tcctgaccat
     1021 cgaacactat ggcgcgggac cctatgcgac cctgctgatg gccgagctgg gcgccgaggt
     1081 gatcaaggtc gaggccccgt cgatgggcgg cgacgcctcg cggccgaccg gacccttcgg
     1141 gctgggcgac aacgacagcc agtatttcca gaccttctcg cgcggcaaga agtcagccgc
     1201 catcgaattg aagaccccgg agggccggac ggacttcgaa cgcctcgtcg cggacgcgga
     1261 agccgtggtc aacaacctgc gcggcgacca gccggcgaag ctgggcctga cctacgcagc
     1321 gctgaaggcg atcaacccgg ccatcgtctg cgggcatctg tccgcctacg gacggaacaa
     1381 cagtcgtgag gcctggcccg gctacgacta cccgatgcag gccgagtgcg gcctgatgag
     1441 cctgaccggc gaaccggacg gcccgcccgc gcgcatgggc gtgtcgatca tcgacttcat
     1501 gagcggcgcg accttcgcct tcggggtggt cagcgccatc ctctcggcgc gagcgacggg
     1561 cgagggctgc gacgtcgacg tctcgctgtt cgacgtggcc ctgcaccagc tcagctatcc
     1621 ggccgtctgg gcgatgaacg agcgccatcc gacggtgcgc atgcccaacg gcgcccatcc
     1681 gtccctcgcc cccgtgcagc gggtgaagac cgccgacggc tatggccagc tgatgtgcat
     1741 gaacgagaag ttctgggccg agttctgcga cacgaccggc cgcgtcgacc tggccgccga
     1801 cccgcgcttc gcgaccctcg ccgaccgcgc ccgcaacctg ccggccctga cgcaggcgct
     1861 cgacgccctg ttcgcgaccc ggccgacggc ccactggagc gccctgttcg cgggccgcgt
     1921 gccgttcggg ccggtcaacg gcctgttcga ggccctcgac aatccgttcg tggcggaagt
     1981 ggccatgcgc gacgtggtcg accatcccga ccgcccccag ggcctgcgga tgctggccat
     2041 gccggtgaag atcgacggcg cgcggatgcc cggcttcagc cgcgaggcga atgcgggcga
     2101 aggcctcgac gatatcgcgg gtgacgccct cgccggagag atagc
//
LOCUS       DAORNX010000492         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38347_length_2144_cov_5.970799, whole genome shotgun sequence.
ACCESSION   DAORNX010000492 DAORNX010000000
VERSION     DAORNX010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38347_length_2144_cov_5.970799"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLO65_15675"
     CDS             <1..576
                     /locus_tag="PLO65_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRD29740.1"
                     /translation="DRAAWLAYLKSRLLLPRADRPKADEAPPEEIAAQLAFRLAKLDA
                     MRRAVDALNARPQLRRDVFVRGDPQAVTILSSGRIEGDLYQLMSAYISQRKREHDRSY
                     RPAVPTAYALDDARHRLRGMLPELAAWTALAGVAPMAVDTQGPSRASYLASTLSASLE
                     LVKEGELEARQLEAFADLYLRRRKRKPEATA"
     gene            573..1232
                     /gene="scpB"
                     /locus_tag="PLO65_15680"
     CDS             573..1232
                     /gene="scpB"
                     /locus_tag="PLO65_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRD29741.1"
                     /translation="MSDSRFIERCIEALLFAAAEPLSLEDLARRLPEGADVAAGLESL
                     RAGCEGRGIELACVAGRWRFQTAADLAFLMTEERDAPRRLSKAAQETLAIIAYHQPVT
                     RAEIEAVRGVQASSGTLDILLELGLVRMRGRRRTPGRPVTYGTTDAFLEHYGLASLAD
                     LPGMADMKAAGLLGLDLPPGFNVPDPARAGEEEDALDFEGGEGEAPEFVQDYVGGEEP
                     G"
     gene            1465..1680
                     /locus_tag="PLO65_15685"
     CDS             1465..1680
                     /locus_tag="PLO65_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRD29742.1"
                     /translation="MGGLSIWHWLIVIAVVAVVFGGRGKLSGLMGDAAKGIRAFRDGL
                     KGEDDKVDETPAKPLPRTETEKDKIVS"
     gene            1737..>2144
                     /gene="tatB"
                     /locus_tag="PLO65_15690"
     CDS             1737..>2144
                     /gene="tatB"
                     /locus_tag="PLO65_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRD29743.1"
                     /translation="MLPEIGASELLVIAIVALIVVGPKDLPVLLRKLGQWVAKMRGMA
                     NEFRASFDEMARQSELDDLRREVEAMRSGTYLSPTPTDEAGTRQVFADIEASLQGGGV
                     QFPPVGAPAVEIEANRTIASPAPAKRKRAPKAAS"
BASE COUNT          317 a          774 c          751 g          302 t
ORIGIN      
        1 gaccgcgccg cctggctggc ctacctcaag tcgcgcctgc tgttgccgcg ggccgaccgg
       61 ccgaaggccg acgaggcgcc gccggaggag atcgccgccc agctggcctt ccgcctggcc
      121 aagctcgacg ccatgcgccg cgccgtcgac gccctcaacg cccggccgca gttgcgccgt
      181 gacgtgttcg tccgggggga tccgcaggcg gtgaccatcc tctcctccgg ccgcatcgag
      241 ggcgacctgt atcagctgat gagcgcctac atcagccagc ggaagcggga gcacgaccgc
      301 agctatcgtc ccgccgtccc gaccgcctac gccctcgacg acgcccgtca ccggcttcgc
      361 ggcatgttgc cggaactggc cgcctggacc gccctggccg gtgtcgcgcc gatggcggtc
      421 gacacccagg gacccagccg cgcctcctat ctcgcctcga ccctctcggc gagcctcgag
      481 ctggtcaagg aaggcgaact cgaagcccgc cagctggaag ccttcgccga cctctacctg
      541 cgcaggcgca agcggaagcc cgaggcgacg gcttgagcga ctcccggttc atcgagcgct
      601 gcatcgaggc gctgctgttc gcggccgccg agccgctgag cctggaggat ctggcgcgcc
      661 gcctgcccga aggcgccgac gtcgcggccg gccttgagtc attgcgcgcc ggttgcgagg
      721 ggcgcgggat cgaactggcc tgcgtcgccg gccgctggcg gttccagacg gccgcggacc
      781 tggcgttcct gatgaccgag gagcgcgatg cgccgcgccg cctgtcgaag gcggcgcagg
      841 agaccctggc gatcatcgcc tatcatcagc cggtcacccg cgccgagatc gaggcggtcc
      901 gcggcgttca ggccagcagc ggcacgctgg acatcctgct ggaactcggt ctggtgcgga
      961 tgcggggtcg gcgccgcacg ccgggccggc cggtgaccta cggcaccacc gacgcctttc
     1021 tcgaacacta tggcctggcc agcctggccg accttccggg catggccgac atgaaagccg
     1081 ccggcctgct cggtctcgac ctgccgcccg gcttcaatgt gcccgacccg gcccgcgccg
     1141 gcgaggaaga ggacgccctc gatttcgagg gcggggaggg ggaggcgccc gagttcgtgc
     1201 aggactatgt cggcggggag gagccgggat aggccatccg gcgcgccctg agacgcgatc
     1261 ccgacggacc ggccctcggg gtgacgaacg ccggaggttg tgctggcgag gcgtgatcct
     1321 ccgcgcgcgt gtgtccagcg ccacacagga ccgtgtcggc cctttaattt ggcgacaggc
     1381 cccgcaaccg cctatattgg gcccaacaat cgcatcgcgc gggcggccgg agcgccgccc
     1441 gactttagga gcaacgccgc catgatgggt ggactgagta tctggcactg gctgatcgtg
     1501 atcgcggtcg tggccgtggt cttcggcgga cggggcaagc tgtccggcct gatgggcgac
     1561 gcggccaagg gcattcgcgc cttccgggac ggcctgaagg gcgaggacga caaggtcgac
     1621 gagaccccgg ccaagccgct gccccgcacc gaaaccgaga aggacaagat cgtcagctga
     1681 cgatcctgcc gtttcgccct tgatcgcgcc ccccgcgcgg gcttaaggcc gccgccatgc
     1741 tgcccgaaat cggcgcttcc gagctccttg tcatcgccat cgtcgccctg atcgtggtgg
     1801 gtcccaagga cctgcccgtc ctgctgcgca agctgggcca gtgggtcgcg aagatgcgcg
     1861 gcatggccaa cgagttccgc gccagcttcg acgagatggc ccgccagtcg gagctggacg
     1921 acctgcgtcg cgaggtggag gccatgcgct cgggaaccta tctcagcccc acgccgaccg
     1981 acgaggccgg gacccggcag gtgttcgcgg acatcgaggc cagcctgcag ggcggcggcg
     2041 tccagtttcc cccggtcggc gccccggcgg tcgagatcga ggctaaccgg accatcgcct
     2101 cgccggcccc ggcgaaacgc aagcgcgcgc cgaaagcggc ttcg
//
LOCUS       DAORNX010000493         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38398_length_2142_cov_8.119310, whole genome shotgun sequence.
ACCESSION   DAORNX010000493 DAORNX010000000
VERSION     DAORNX010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38398_length_2142_cov_8.119310"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..>2142
                     /locus_tag="PLO65_15695"
     CDS             272..>2142
                     /locus_tag="PLO65_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRD29744.1"
                     /translation="MTDKPFQHAPDAPWIFRTYAGHSTAEKSNALYRRNLAAGQTGLS
                     VAFDLPTQTGYDADHILARGEVGKVGVPVGHLGDMRALFDGIPLDRMNTSMTINAPAA
                     WLLAMYVALADEQGAPRSALQGTTQNDLIKEYLSRGTYIFPPEPSLRLIGDMIAWCYR
                     EVPRWNPMNVCSYHLQEAGATPEQELAYALATAISVLDTVRAGGQVPDEDFEIVCSRI
                     SFFVNAGVRFVTELCKMRAFTALWDEILRDRYGVKDAKARRFRYGVQVNSLGLTEQQP
                     ENNPYRILLEALAVTLSKDARCRALQLPAWNEALGLPRPWDQQWSLRMQQVLAYETDL
                     LEYEDLFAGSHVVEAKVAELKAGALSELARIDGIGGAAAAIDYMKAELVGSNAARVRA
                     VETGGMTVVGVNRWTDTEQSPLSAGEGSIETVDPKLEAGVVERLKAWRAARDNAAVEA
                     ALADLKAAARDGRNIMEPSIVAAKAGVTTGEWAFALREVFGEYRGPTGVAVVVASGDD
                     AEDIEAVRKDVERVSEVLGRTLTYLIGKPGLDGHSNGAEQIATRARAVGMEVHYDGIR
                     STPDEIVRRAKETKAHVVGLSILSGSHLDLVQEVVRVMRAEGLGDVPVVVGGIIPPD"
BASE COUNT          337 a          739 c          761 g          305 t
ORIGIN      
        1 aagctgttcg cgggactgga cgcggccgag agcgcggcgc gcctgccggg gacactggat
       61 ctgctggccg gcagcgccga ccatcgcgag gcggtcgccg ccttcctcga gaagcgggcg
      121 gcggtgtttc gccgctcgta gccgggtccc ggatcggtct gacggccgtc tagcgggcat
      181 caagggtcat tcttgccgcg cctgacgctc gcgtaagccg ttcgcgcaaa aatgttgcac
      241 cgcagcaggc ggatcgccat gatccgccgc catgaccgac aagcccttcc agcacgctcc
      301 cgacgctccg tggatcttcc gcacctatgc gggtcactcc acggccgaga agtccaacgc
      361 cctgtaccgg cgcaacctgg cggccgggca gaccgggctg tcggtggcct tcgacctgcc
      421 gacccagacc ggctacgacg ccgaccacat cctggcgcgc ggcgaggtcg gcaaggtcgg
      481 ggtgccggtc ggccacctgg gcgacatgcg ggcgctgttc gacggcattc cgctcgaccg
      541 gatgaacacc tcgatgacga tcaacgcccc ggccgcctgg ctgctggcga tgtacgtcgc
      601 cctggccgac gagcaggggg cgccgcggtc cgcgctgcag ggcacgacgc agaatgatct
      661 gatcaaggag tatctcagcc gcgggactta catcttcccg cccgagccca gcctgcggct
      721 gatcggcgac atgatcgcct ggtgctaccg cgaggtgccc aggtggaacc cgatgaacgt
      781 ctgcagctac cacctgcagg aggccggggc gacgccggag caggaactgg cctacgccct
      841 ggccacggcc atctcggtgc tcgacacggt cagggccggc ggccaggtcc ccgacgagga
      901 cttcgagatc gtctgttccc ggatcagctt cttcgtcaac gccggcgtcc ggttcgtcac
      961 cgagctgtgc aagatgcgcg cgttcaccgc cctgtgggac gagatcctca gggaccgcta
     1021 cggtgtcaag gacgccaagg cccgccgctt ccgctacggg gtgcaggtca acagcctggg
     1081 cctgaccgag cagcagccgg agaacaaccc ctaccgcatc ctgctcgagg cgctggcggt
     1141 gacgctgagc aaggacgccc gctgccgggc cctgcagctg ccggcgtgga acgaggcgct
     1201 gggcctgccg cggccatggg atcagcagtg gtccctgcgc atgcagcagg tgctggccta
     1261 tgagaccgac ctgctggaat acgaggacct gttcgccggc agccacgtgg tcgaggccaa
     1321 ggtcgccgag ctgaaggccg gggccctgtc cgagctggcc cggatcgacg ggatcggcgg
     1381 cgccgcggcg gccatcgact acatgaaggc cgagctggtc gggtccaacg cagcccgggt
     1441 gcgcgccgtc gagaccggcg gcatgacggt ggtcggggtc aaccgctgga cagacaccga
     1501 acagtcgccc ctctcggccg gcgagggctc gatcgagacg gtcgatccaa agctcgaggc
     1561 gggcgtcgtc gagcggctga aggcctggcg cgcggcgcgg gacaacgccg ccgtcgaggc
     1621 cgccctggcc gacctgaagg ccgccgcccg cgacggtcgc aacatcatgg agccctcgat
     1681 cgtcgcggcg aaggccggcg tgaccaccgg cgagtgggct ttcgccctgc gcgaggtgtt
     1741 cggcgaatac cgcggcccga cgggcgtcgc cgtggtcgtc gcttccggcg acgacgccga
     1801 ggatatcgag gcggtcagga aggacgtcga gcgcgtctcc gaagtgctcg gccgcaccct
     1861 gacctacctg atcggcaagc cgggtctcga cggccattcc aacggcgctg aacagatcgc
     1921 cacccgcgcc cgcgcggtcg gcatggaggt gcattacgac ggcatccgtt cgaccccgga
     1981 cgagatcgtc cgccgggcga aggagacgaa ggcccatgtc gtcggcctgt cgatcctcag
     2041 cggctcgcat ctggacctcg tccaggaggt ggtccgggtg atgcgcgccg aggggctggg
     2101 cgacgtgccc gtggtggtcg gcggcatcat cccgccggac ga
//
LOCUS       DAORNX010000494         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38441_length_2140_cov_6.692086, whole genome shotgun sequence.
ACCESSION   DAORNX010000494 DAORNX010000000
VERSION     DAORNX010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38441_length_2140_cov_6.692086"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="gatB"
                     /locus_tag="PLO65_15700"
     CDS             complement(<1..472)
                     /gene="gatB"
                     /locus_tag="PLO65_15700"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517891.3"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRD29745.1"
                     /translation="MSDSSKTLQGRTGPWEIVLGLEVHAQVASNSKLFSGAAVGFGAG
                     PNEQVSLVDAAMPGMLPVLNRYCVEQAVRTGLGLKAQINLKSTFDRKNYFYPDLPQGY
                     QISQFKSPIVGEGLVTVDRDDGSSFAVRIERLHLEQDAGKSIHDLSPTETYVDLN"
     gene            635..>2140
                     /locus_tag="PLO65_15705"
     CDS             635..>2140
                     /locus_tag="PLO65_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HRD29746.1"
                     /translation="MLSVLSCLREQHDLRLVLIAAVICIASILTAFGLYDRALRTAGP
                     FRAGWLTLTGLIAGSGVWATHFMGMLAYQPDLLVGYDPLGTALSLVASLAGMGLAFTL
                     PAVFGGRVGFLAGGLAAGLALATMHFLGISAAIMPMHILWNPGQVIASIAGGSALTAG
                     AFLLFTRRGDPAWRTASGLLMAGAVLLLHFTAMAAVTLVPDSTIPLGGAQFDRAELAI
                     MTGAFAAGLLLACLALLWVESQSHRGALISLRSSLDALPSGVAFMDAADRLVIWNLAF
                     ADLAACWGLVPEVGARGEVLRAVCGADTAPDGDVEKRSDGRWIRAMTSATPEGGSVLI
                     LRDITADREHAAAMAAARDEAQAASRAKSDFLANMSHEIRTPLNGVMGAAEVLAREPL
                     VGHQAELVQMIHASGDTLNRLLSDILDLARVETGKLDIVAEPFDLAEAVRTAAALFKL
                     KADEKGLTFDLRIAPEAEGRVIGDPIRLRQILGNLLSNAVKFTQAGGVSLTV"
BASE COUNT          296 a          748 c          728 g          368 t
ORIGIN      
        1 ggttgaggtc gacataggtc tcggtcggcg acaggtcgtg gatcgacttg ccggcatcct
       61 gctccaggtg cagccgctcg atgcgcacgg cgaagctgga gccgtcgtcg cggtcgaccg
      121 tgaccagtcc ctcgccgacg atcggcgact tgaactggct gatctgatag ccctgcggca
      181 gatcgggata gaagtagttc ttccggtcga aggtgctctt gagattgatc tgcgccttca
      241 ggccaaggcc ggtgcggacc gcctgttcga cgcagtacct gttgagcacg ggcagcatgc
      301 cgggcatggc cgcatcgacc aggctgacct gctcgttcgg gccggcgcca aagccgacgg
      361 ccgcgccgct gaacagcttg gagttgctgg cgacctgggc gtggacttcc aggccaagga
      421 cgatttccca gggcccggtg cggccctgca gggttttcga agagtcggac atggcccgct
      481 ccatacaacg cctttgcgcc cagtgggagg gcgttgattg accccgggcc ggccgcgacc
      541 gattgacacg cggggcgcgc attttgatct atctcatcct ctcccgtgtc gcgccgccca
      601 tgttcgatga actttggtcc cggggagacc cttcatgctg tccgttctga gctgcctgcg
      661 cgagcagcat gatctgcgtc ttgtgctgat cgccgcggtg atctgcatcg cgtcgatcct
      721 gacagcgttc ggcctctatg atcgggcgct gcgcacggcg ggcccgttcc gcgccggctg
      781 gctgaccctc accggcctga tcgccggatc cggggtctgg gcgacccact tcatgggcat
      841 gctggcctac cagcccgacc tgctggtcgg atacgaccct ctgggcaccg cactgtcgct
      901 ggtcgcctcg ctcgccggca tgggcctggc cttcaccctt cccgccgtgt tcggcggtcg
      961 agtgggattt ctcgccggcg ggctcgccgc cggcctggcc ctggccacca tgcatttcct
     1021 tggcatttcg gcggcgatca tgccgatgca tatcctctgg aacccgggcc aggtcatcgc
     1081 ctcgatcgcc ggaggatccg cgctgacggc cggggccttc ctgctgttca cccgacgcgg
     1141 cgacccggcc tggcggacgg ccagcggcct gctgatggcc ggcgcggtgc tcctgctgca
     1201 tttcacggcc atggcggcgg tgaccctcgt gccggacagc accatcccgc tgggcggagc
     1261 ccagttcgac cgggccgaac tggcgatcat gacgggcgcg ttcgccgccg gcctgctgct
     1321 ggcctgcctg gccctgctgt gggtcgagag ccagagccac cggggcgccc tgatcagcct
     1381 gcgctcgtcg ctcgacgccc taccgtcggg cgtggccttc atggacgccg ccgaccgcct
     1441 ggtgatctgg aacctcgcct tcgccgacct cgccgcctgc tggggcctgg ttccggaggt
     1501 cggcgcccgg ggcgaagtcc tgcgcgcggt ctgcggcgcc gacaccgctc cggacggcga
     1561 cgtcgagaaa cgctccgacg gccgctggat tcgcgcgatg accagcgcca cgccggaggg
     1621 cgggtcggtg ctcatcctgc gggacatcac cgccgaccgt gagcatgccg cggccatggc
     1681 cgccgcccgc gacgaggccc aggcggccag ccgcgccaag agcgacttcc tcgccaacat
     1741 gagccatgaa atccgcaccc cgctgaacgg ggtgatgggg gcggccgagg tgctggcccg
     1801 ggaaccgctg gtcggacacc aggccgaact ggtgcagatg atccacgcct cgggggacac
     1861 cctgaaccgc ctgctcagcg acattctcga tctcgcgcgc gtcgagaccg gcaagctcga
     1921 catcgtggcc gaaccgttcg atctcgccga ggcggtgcgc acggccgccg ccctgttcaa
     1981 gctcaaggcc gacgaaaagg gcctgacctt cgacctgcgg atcgcgccgg aggccgaggg
     2041 ccgggtgatc ggcgatccga tccggctgcg ccagattctc ggcaacctgc tgagcaacgc
     2101 ggtgaagttc acccaggcgg gcggcgtgag cctgaccgtc
//
LOCUS       DAORNX010000495         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38465_length_2139_cov_7.032630, whole genome shotgun sequence.
ACCESSION   DAORNX010000495 DAORNX010000000
VERSION     DAORNX010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38465_length_2139_cov_7.032630"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLO65_15710"
     CDS             complement(<1..192)
                     /locus_tag="PLO65_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29747.1"
                     /translation="MAGKRRKAGRAGEPSFAASQSRAPSGAFVRIAILAALLLLAVYS
                     AFGVMRLERGWVSRDGSGGP"
     gene            437..1324
                     /locus_tag="PLO65_15715"
     CDS             437..1324
                     /locus_tag="PLO65_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD29748.1"
                     /translation="MTTTPLSLRHALLALAVMAVWGSNFVVIRVGLDHLPPLLFAALR
                     FSVAFLPAALFLKKPDVPWRNLAAYGVLIGAGQFGLLFLAMKGHISPGLASLVVQTQA
                     FFTIGLAMRLTGERVRTFQYVALALAAAGIGVIIWRTDGSATPLGLALVLGAALCWAG
                     GNLVARRTPTVNMLAYVVWASLFSVPPLLTLAVVFEGWPAIVAGVTRADALTWAAVVW
                     QAVGNTMFGYAVWGWLLARYPAATVAPMSLLVPVFGMAASAVFLHEALPAWKLAAAGL
                     VMAGLALNTFWPRLLRQRL"
     gene            complement(1307..>2139)
                     /locus_tag="PLO65_15720"
     CDS             complement(1307..>2139)
                     /locus_tag="PLO65_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HRD29749.1"
                     /translation="VTTPGSLAGLFEFLDDAAPDALFGYNHPGREPGALDDFSLPPSH
                     FRHLQRRMVALEAFNRREDFLFQPRLGGPFSPLAEAAGRRALRNAALDLLSVDSHAEN
                     VDIAARHFETAANMTDAIGGLAALMALGGDGREKALETFHARWKDEPLVLDKWFAIQA
                     RDSSDEALGRVLGLTVHPDFDRRNPNRLRALVGTFASFNPARFHDPSGAGYRFLADQI
                     IATDKVNPMTAARFIEPLGGWRRYQPELGALMKAQLERIVAVEGLSKNVFELASKALA
                     "
BASE COUNT          291 a          735 c          749 g          364 t
ORIGIN      
        1 gggcccgccc gagccgtcgc gactgaccca gccgcgctcg agccgcatga cgccgaacgc
       61 cgaatagacc gccagcagca gcagggcggc gaggatggcg atgcggacga aggcccccga
      121 gggggcgcgg ctctgcgacg cggcaaagga tggctcgccg gctcttcctg ccttgcgacg
      181 cttgcccgcc aacctgtcgt ccccaagccc gagtcagttt ccccgtgcag ggaatctagc
      241 ggaagccggg tccttccgtc acgggccaaa gggggttttg tccccggcct gagtcagatg
      301 gccgcggacc gtgtttcagc ggactcaccg ggaccgccgg cgagaaggaa acctcgcgtc
      361 cttgccagca gaattgccgt gtcgtggggc cgcaaacacc ctaaaccggg ccggccgccg
      421 cgaggagccc gggcccatga ccaccacccc cctgtccctg cgccacgccc tgctggccct
      481 ggcggtgatg gcggtctggg ggtccaactt cgtggtcatc cgcgtcggtc tcgaccattt
      541 gccgcccctg ctgttcgccg cgctgcggtt cagtgtcgcc ttcctgccgg cagccctgtt
      601 cctgaagaag ccggatgtgc cctggcgcaa ccttgcggcc tatggcgtgc tgatcggggc
      661 cggccagttc ggcctgctgt tcctcgccat gaagggtcac atctcgccgg gcctcgcctc
      721 gctggtggtg cagacccagg ccttcttcac catcggactg gccatgcggc tgaccggcga
      781 gcgggtcagg accttccagt atgtcgccct ggccctggcc gccgccggca tcggggtgat
      841 catctggcgg accgacggtt cagccacgcc gctcggcctg gcgctggtgc tgggcgcggc
      901 gctttgctgg gccggaggca atctggtggc ccgccggacg ccgacggtga acatgctggc
      961 ctatgtcgtc tgggccagcc tgttctcggt tccgccattg ctgaccctgg ccgtggtgtt
     1021 cgagggctgg ccggcgatcg tcgcgggcgt aacgcgcgcc gacgccctga cctgggcggc
     1081 cgtggtctgg caggccgtgg gcaacaccat gttcggctac gctgtctggg gctggctgct
     1141 ggcccgctat ccggccgcca cagtcgcgcc gatgtcgctg ctggtgccgg tattcggcat
     1201 ggccgcctcg gcggttttcc tgcatgaggc cctgccggcc tggaagctgg ccgccgccgg
     1261 cctggtcatg gccggcctgg ccctgaacac cttctggccg cggcttctac gccaacgcct
     1321 ttgacgccag ctcgaagacg ttcttggaca ggccttccac cgcgacgatc cgctccagct
     1381 gcgccttcat cagcgcgccg agttccggct ggtagcgacg ccagccgccg agcggttcta
     1441 tgaagcgcgc ggcggtcatc gggttcacct tgtccgtggc gatgatctgg tcggccagga
     1501 aacgatagcc ggcgcccgac ggatcgtgga agcgggccgg attgaagctg gcgaaggttc
     1561 ccaccagggc gcgcaggcgg ttgggattgc gtcggtcgaa gtcgggatgg acggtcaggc
     1621 ccagcaccct gccgagggcc tcgtccgaac tgtcgcgcgc ctggatggcg aaccatttgt
     1681 ccagcaccag cggttcatcc ttccagcggg cgtggaaggt ttcgagagcc ttttccctgc
     1741 cgtcgcctcc cagcgccatc agggccgcca ggccgccgat cgcgtcggtc atgttggccg
     1801 cggtttcaaa gtgccgcgcg gcgatgtcga cgttttcggc gtggctgtcg accgacagca
     1861 gatcgagggc cgcgttgcgc agggcccggc gccccgccgc ttcggccagg ggcgagaacg
     1921 ggccgccgag gcggggctgg aagaggaagt cctcgcggcg gttgaacgcc tccagcgcca
     1981 ccatccgtcg ctggagatga cggaaatgac tggggggcaa cgagaaatcg tccagtgcgc
     2041 ctggttcccg accggggtgg ttgtagccga acagcgcatc cggcgcggcg tcgtccagga
     2101 actcgaacag gccggccagc gacccgggcg tggtgaccg
//
LOCUS       DAORNX010000496         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38466_length_2139_cov_5.838772, whole genome shotgun sequence.
ACCESSION   DAORNX010000496 DAORNX010000000
VERSION     DAORNX010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38466_length_2139_cov_5.838772"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2139
                     /locus_tag="PLO65_15725"
     CDS             <1..>2139
                     /locus_tag="PLO65_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase TIM-barrel-like
                     domain-containing protein"
                     /protein_id="HRD29750.1"
                     /translation="ALSALQPARQVGPRLRELQLMSAAEGAPMAAVFGRARVAGQVIW
                     AARFKERRIERQAGGGKGGPRTVDYRYSLSFAVALCEGPIDGVGRIWADGRPMDMAGV
                     TMRVHRGGEDQAPDPLIEAIEGASPAYRGCAYVVFEDLPLEAYGDRPPQLSFEVFRRP
                     ASTGGLEDRLTGVCLIPGAGEFVYATDVIQRRDGLTRLTAENVNNGEGRPDLLVSLDQ
                     LQAQLPRLEEVTLVVAWFGDSLDAGLCRFRPGVERITKATRPWSWRAGGVGRGGAHVV
                     SQQDGGPSYGGTPADRAVLQAIAELKARGLRVTLYPFILMDCEAYPWRGRISGEAADI
                     PALFGGASADDFTIDGESVAYGGPTDWGLRRMVLHYARLAQAAGGVDGFLIGSELRGI
                     TAIRDGAGFPAVEALRVLATDCRSILGPEAAIGYAADWSEYFGHQPADGSGDVFFHLD
                     PLWADPAIDFVGIDFYPPLADWRDGEPQDAWDVASGEGYDWYYASEADRLARLRSPIT
                     DVAHGEPWVFRPKDLAGWWSNPHHDRPAGVRSGAPTAWIPCSKPIRLIEFGCAAVDRG
                     ANAPNLFVDPKSSESALPPFSSGRRDDAGQRRTLEAVLAHFADVANNPVSPVYGGPMV
                     SAMGAWCWDARPFPDFPARAGVWRDGANWVTGHWLNGRAGTAPLAELVVALAERAGVT
                     IDPGALGGMVSGYVVEAPMRLRDALSPLAL"
BASE COUNT          270 a          754 c          786 g          329 t
ORIGIN      
        1 cggccctctc ggcgctccaa ccggccaggc aggtcgggcc gcggctgcgc gagttgcaac
       61 tgatgtccgc cgccgagggc gccccgatgg ccgccgtgtt cggtcgggcc cgcgtggccg
      121 gccaggtgat ctgggcagcc cgcttcaaag aacgacggat cgagcgccag gccggcggcg
      181 gcaagggcgg gccgcgcacc gtcgactacc gctacagcct gtcgttcgcc gtagccctct
      241 gcgaggggcc gatcgacggc gtgggccgga tctgggccga cggccggccg atggacatgg
      301 ccggcgtgac catgcgggtc catcgcggcg gcgaggacca ggcgcccgat ccgctgatcg
      361 aggccatcga gggcgcgtca cccgcctatc ggggctgcgc ctatgtcgtc ttcgaggatc
      421 tgcccctgga ggcctatggc gaccggccgc cgcaactgtc gttcgaggtg ttccgccggc
      481 cggcatcgac cggcggcctc gaggaccggc tgaccggcgt gtgcctgatc cccggcgccg
      541 gggagttcgt ctacgccacc gatgtgatcc agcgccgcga cggcctgacc cgcctgacgg
      601 ccgagaacgt caacaatggc gaggggcgac ccgacctgct cgtgtcgctt gaccagctgc
      661 aggcgcaact gccgcgtctc gaggaggtga ccctcgtggt cgcctggttc ggagacagcc
      721 ttgacgccgg cctctgccgg ttccgaccgg gcgtcgagcg gatcaccaag gcgacccggc
      781 cgtggtcgtg gcgcgcgggc ggcgtcggcc ggggcggtgc gcacgtcgtc tcgcaacagg
      841 atggaggccc ctcctacggc ggcacgccgg ccgaccgcgc cgtgctgcag gccatcgccg
      901 agctgaaggc ccgaggcctg cgggtgaccc tctacccgtt catcctgatg gactgcgaag
      961 cctatccgtg gcgcggaaga attagtgggg aagccgctga tattccagcc cttttcggcg
     1021 gagcctcggc tgatgatttc accatcgacg gagaatcggt cgcctacggc gggccgaccg
     1081 actgggggct gcggcggatg gtgctgcact acgcccgcct ggcccaggcg gccggcggcg
     1141 tggacggatt cctgatcggc tcggagcttc gcgggatcac cgccatccgg gatggcgccg
     1201 gcttccccgc cgtcgaggcg ctgcgtgttc tggcgacgga ttgccggtcg attctggggc
     1261 cggaggcggc gatcggctac gcggccgact ggagcgagta tttcggccat cagccggccg
     1321 acgggtccgg cgacgtgttc ttccatctcg atccgctgtg ggccgatccc gccatcgact
     1381 tcgtcggcat cgacttctat ccgccgctgg ccgactggcg cgacggcgag ccgcaggacg
     1441 cctgggatgt cgcgtccggc gaaggctacg actggtacta cgcctcggag gcggaccggc
     1501 tggcgcggct gcgctccccg atcaccgacg tcgcccatgg cgagccctgg gtgttccggc
     1561 ccaaggacct ggccggctgg tggtccaacc cgcatcacga ccgacccgcc ggcgtacggt
     1621 ccggcgcgcc gaccgcctgg attccctgct ccaagccgat ccggctgatc gagttcggct
     1681 gcgcggccgt cgaccggggg gccaatgcgc cgaacctgtt cgtcgatccc aagagcagcg
     1741 agagcgccct gccgccgttt tccagcggcc ggcgcgacga cgcgggccag cggcgcacgc
     1801 tggaggccgt gctggctcat ttcgccgacg tcgccaacaa tcccgtctcg ccggtctatg
     1861 gcgggccgat ggtttcggcg atgggcgcct ggtgctggga cgcccggccg tttcccgact
     1921 tccccgcccg cgccggtgtc tggcgcgacg gggccaactg ggtcaccgga cactggctga
     1981 acggccgggc cgggacggcg cccctggccg agctggtcgt ggcgctggcc gaacgggccg
     2041 gtgtcacgat cgatcccggc gcgctgggcg gcatggtcag cggctatgtc gtcgaggcgc
     2101 cgatgcgcct gcgtgacgcg ctgtcgccgc tggcgctgg
//
LOCUS       DAORNX010000497         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38538_length_2136_cov_6.862566, whole genome shotgun sequence.
ACCESSION   DAORNX010000497 DAORNX010000000
VERSION     DAORNX010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38538_length_2136_cov_6.862566"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLO65_15730"
     CDS             <1..982
                     /locus_tag="PLO65_15730"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29751.1"
                     /translation="TAAPAAPDTTVDAAWSQAGPYRVVQLEGEWRDQARDRTVPWLIR
                     YPEGVSGAAPVVVFSHGLGGSRRGAAYYGHFLASHGYVVVMVQHPGSDVSIWQGVRPD
                     LGKVDRAMLARAVADPRVTLNRFFDIPFALDQLALMNRADGPLKGRMDLARIGMSGHS
                     FGAVTTQAMAGQVYPGGRSLPEPRFTAFLAMSPSAARDGDNATAFGSITRPFLSLTGT
                     EDSFTVRADEPAADVVAGRQQPFAHVAGPDMLVLLTGGDHMVFSGRQEVGGAPRPNDA
                     RHRQIILAASLAWWDAWLKDDPKALAWLRDGGLAQFAGADATVSTRGPDH"
     gene            1024..1539
                     /locus_tag="PLO65_15735"
     CDS             1024..1539
                     /locus_tag="PLO65_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD29752.1"
                     /translation="MPAPTIRRATPADAEALSHIGAETFTETFGHLYPPQDLADFLVY
                     AYNLDRTRADLADPAKASWVVEADGAVVGYALAGPCDLPHEEVTPACGELKRLYVLKA
                     FQGGGTGSRLLAEVLAWLEKDGPRPLWIGVWSENFGAQRLYGRMGFDKVGEYAFAVGQ
                     TLDREFILRRG"
     gene            1725..>2136
                     /locus_tag="PLO65_15740"
     CDS             1725..>2136
                     /locus_tag="PLO65_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRD29753.1"
                     /translation="MFMNFFTALREAKVPVTLKEYLMLMEAMDKDVIDRKVEDFYYLS
                     RSALVKDEKNLDKFDQVFGHVFKGLEMVGDAGNPEIPAEWLKLLTEKFLTDEEKAEIE
                     AMGGFDKLMEMLQKRLEEQKKRHEGGNKMIGTGGT"
BASE COUNT          337 a          732 c          740 g          327 t
ORIGIN      
        1 cacggccgcg ccggccgcgc cggacacgac cgtcgacgcc gcctggagcc aggccgggcc
       61 gtaccgtgtg gtccagctgg agggcgagtg gcgcgatcag gcgcgggacc ggaccgtgcc
      121 ctggctgatc cgctatccgg aaggcgtgtc aggcgcagcg ccggtggtgg tgttctcgca
      181 cggactgggc ggcagccggc gcggcgcggc ctactacggc cacttcctcg ccagccacgg
      241 ctacgtcgtg gtgatggtgc agcatccggg ttccgacgtg tcgatctggc agggcgtgcg
      301 gccggacctc ggcaaagtcg accgggccat gctcgcccgc gcggtcgccg atccgcgggt
      361 cacgctgaac cgcttcttcg acatcccgtt cgcgctcgac cagctggccc tgatgaaccg
      421 ggcggacggg ccgctgaagg gtcgcatgga cctggcccgc atcggcatgt ccggccacag
      481 cttcggcgcg gtgacgacac aggccatggc cggccaggtc tatccgggcg gccgctccct
      541 gcccgagccg cgcttcaccg ccttcctggc catgagcccg tccgccgccc gcgacggcga
      601 caacgcgacg gccttcggat cgatcacccg gccgttcctg tcgctgaccg gcacggagga
      661 cagtttcacg gtccgcgccg acgagcccgc cgccgatgtc gtcgccggcc ggcaacagcc
      721 gttcgcccat gtcgccgggc cggacatgct ggtgctgctg accggcggcg accacatggt
      781 cttttccggc cggcaggagg tcggcggcgc gccccggccc aacgacgcgc gccaccgcca
      841 gatcatcctg gccgcgtcgc tggcctggtg ggacgcgtgg ctgaaggacg atccgaaggc
      901 gttggcctgg ctgcgcgacg gcggcctggc gcagttcgcg ggcgcggacg cgacggtcag
      961 cacgcgcggg ccggatcact gaccggacct tgcgcggcga acggcgaacg ttattcaacc
     1021 ctcatgcccg ctccgaccat tcgccgcgcc acgcccgccg acgccgaagc cctgagccat
     1081 atcggcgcgg agaccttcac cgagaccttt ggccatctct accctccgca ggatctcgcc
     1141 gatttcctcg tctatgccta caacctggat cgcacgcggg ccgatctggc cgatccggcg
     1201 aaggcgtcct gggtggtcga ggccgatggc gccgtcgtcg gctacgccct ggccggcccc
     1261 tgcgacctgc cccatgagga ggtcaccccg gcctgcggcg agctcaagcg gctctatgtg
     1321 ctgaaggcct tccagggcgg cggaaccggc tcgcgcctgc tggccgaggt gctggcctgg
     1381 ctggagaagg acggcccccg cccgctgtgg atcggcgtct ggtcggagaa cttcggggcg
     1441 cagcggctct acggccggat ggggttcgac aaggtcggcg aatacgcgtt cgccgtcggc
     1501 cagaccctcg accgcgagtt catcctgcgg cgggggtgag gctgattgcg tccttcgaca
     1561 cggccctgcg ggcctgctca ggatgacgtg catttgctct acgtcatggt gagcagcgcc
     1621 gaaggcgcgt gtcgaaccac gcacagcgca ttcgcgaaac tggacaaggc ggcggttgcc
     1681 ccccatcctc tgtcaaacaa ccgttcgacg cctgagagtc cgccatgttc atgaacttct
     1741 tcaccgcgct gcgggaggcc aaggtccccg tcacgctgaa ggaatacctg atgctcatgg
     1801 aggcgatgga caaggacgtc atcgatcgca aggtcgagga cttctactac ctgtcgcgtt
     1861 cggcgctggt gaaggacgag aagaacctcg acaagttcga ccaggtgttc ggccatgtgt
     1921 tcaagggcct ggagatggtc ggcgacgcgg gcaatccgga aatcccggcc gagtggctca
     1981 agctgctgac cgagaagttc ctgaccgacg aggagaaggc cgagatcgag gccatgggcg
     2041 gcttcgacaa gctcatggag atgctgcaga agcggctcga agagcagaag aagcgccacg
     2101 agggcggcaa caagatgatc ggcaccggcg gcacca
//
LOCUS       DAORNX010000498         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38556_length_2135_cov_8.474519, whole genome shotgun sequence.
ACCESSION   DAORNX010000498 DAORNX010000000
VERSION     DAORNX010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38556_length_2135_cov_8.474519"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLO65_15745"
     CDS             complement(<1..264)
                     /locus_tag="PLO65_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRD29754.1"
                     /translation="MWASLMALTILLVALPALAAAPTFPPLTGRVVDDANVLSPATEA
                     ALTEKLAGLEAASGHQLVVVTLPTLQGYEIEDYGYQLGRAWKIG"
     gene            complement(320..970)
                     /locus_tag="PLO65_15750"
     CDS             complement(320..970)
                     /locus_tag="PLO65_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRD29755.1"
                     /translation="MTIFSRLVRIVPALAIAATLAGCGINTIPTRDEQVKAAWGDVQN
                     QYQRRADLIPNLVNTVKGYAAQEKDVLTAVTEARASATQVRVDASTITNPAQFQQYAA
                     AQDRLSGVLGRLMMIQENYPELKSNQNFLALQSQLEGTENRISVARRDYNEAVRLYNT
                     SLRTFPTVIWAKTLYGAQKPALPFEAAATAQTAPTVSFDTTPPAAAPAPATAPATK"
     gene            complement(1050..1457)
                     /locus_tag="PLO65_15755"
     CDS             complement(1050..1457)
                     /locus_tag="PLO65_15755"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD29756.1"
                     /translation="MAHIEGGCLCGQVRFSGEAEPLFQAVCHCKNCQRQAGSAYSVIV
                     GVPRGALTVTGTLKTYHDRGDSGGTVDRHFCPDCGSPLFSDAPARPELVFIKAGTLDD
                     PSWVTPQIHIWTESAMACTTIPAGVPAFAKNPG"
     gene            complement(1467..>2135)
                     /locus_tag="PLO65_15760"
     CDS             complement(1467..>2135)
                     /locus_tag="PLO65_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD29757.1"
                     /translation="IKIEEWMWTFNMPTDHPRISFDCWIPEDSYWGLVDRGLGLGQSF
                     VHQNRIRQAASSLGAAVYCINESVKYARMRKPFGKALSDNQAIQFPLVELHAQCEMLR
                     LLIRQTAWQMDTMDHKTIEREISDKVSICNYWGNRLCCEAADRAMQVHGGMGYSRHKA
                     FEHIYRHHRRYRITEGSEEIQQRKVAAFLFGYLGPKRQEFKALEEEWAAGRAAGKNDR
                     ELGM"
BASE COUNT          308 a          701 c          755 g          371 t
ORIGIN      
        1 gccgatcttc caggcccggc ccagctggta gccgtagtcc tcgatttcgt agccctgcag
       61 cgtgggaagg gtgaccacca ccagctgatg gccggaggcg gcctcgagcc cggccagctt
      121 ttcggtcagc gccgcctccg tcgccggcga cagcacattg gcgtcgtcga ccacccgtcc
      181 cgtcagcggg ggaaaggtcg gcgcggcggc cagcgccggc agggcgacga gcaggatggt
      241 gagcgccatg agcgacgccc acatccttcc ccacgcggcc gatgaccggt ggggaaggac
      301 cttggtggtg gcgttcaagt tacttcgtcg ccggcgcggt ggccggggcc ggggcggccg
      361 ccggcggcgt cgtgtcgaag ctgaccgtcg gcgcggtctg ggcggtggcc gccgcctcga
      421 acggcagggc cggcttctgg gcgccgtaga gcgtcttggc ccagatcacc gtcgggaagg
      481 tccgcaggct ggtgttgtag agccggacgg cctcgttgta gtcgcggcgg gcgacgctga
      541 tgcggttctc cgtcccctca agctggctct gcagggccag gaagttctgg ttcgacttca
      601 gctccggata gttctcctgg atcatcatca gccggccgag cacgccgctg agcctgtcct
      661 gggccgccgc gtactgctgg aactgggccg ggttggtgat cgtcgaggcg tcgacgcgca
      721 cctgggtggc gctggcgcgg gcctcggtga cggcggtcag cacgtccttc tcctgggcgg
      781 cgtagccctt caccgtgttg accaggttcg gaatcaggtc ggcccggcgc tggtactggt
      841 tctggacgtc gccccaggcg gccttcacct gctcgtcgcg ggtgggaatg gtgttgatgc
      901 cgcagccggc cagggtcgcg gcgatcgcga gcgcggggac gatgcggaca aggcgcgaaa
      961 agatggtcat ggatggcccc ttcaatcggc cgcccgagcg gccgtgaagg cacatatagg
     1021 agagatcgcg gtcgaggggg agaaccccgc tatccaggat tcttcgcgaa ggcggggacg
     1081 ccggccggaa tcgtcgtgca ggccatggcg ctctcggtcc agatatggat ctgcggcgtc
     1141 acccagctgg ggtcgtcaag cgtcccggcc ttgatgaaca ccagctccgg ccgggccggc
     1201 gcgtccgaga acagcggcga gccgcagtcg gggcagaagt gacggtccac ggtcccgccg
     1261 ctgtcacccc tgtcgtgata ggtcttcagc gtcccggtca cggtcagcgc cccgcgcggc
     1321 acgccgacga tcaccgaata ggccgaaccg gcctgccgct gacagttctt gcagtggcag
     1381 acggcctgga acagcggctc ggcctcgccg ctgaaccgca cctggccgca aaggcagccg
     1441 ccctcgatgt gcgccatctg accctcctac atgcccagtt cgcgatcgtt cttgccggcc
     1501 gcgcggccgg ccgcccactc ttcttccagc gccttgaact cctggcgctt cgggccgagg
     1561 taaccaaaca ggaaggcggc gaccttgcgc tgctggattt cctcgctgcc ctcggtgatg
     1621 cggtagcggc ggtggtggcg atagatgtgc tcgaaggcct tgtgccggct gtagcccatg
     1681 ccgccgtgca cctgcatggc gcggtcggcg gcctcgcagc acaggcggtt gccccagtag
     1741 ttgcagatcg agaccttgtc ggagatctcc cgctcgatgg tcttgtggtc catcgtgtcc
     1801 atctgccagg cggtctggcg gatcagcagg cgcagcatct cgcactgggc gtgcagctcg
     1861 acgagcggga actggatcgc ctggttgtcg ctcagcgcct tgccgaacgg cttgcgcatg
     1921 cgcgcgtact tgacgctctc gttgatgcag tagaccgccg cgcccagcga actggccgcc
     1981 tggcggatgc ggttctggtg cacgaacgac tggcccaggc ccaggccccg gtcgaccagg
     2041 ccccagtagc tgtcctcggg gatccagcag tcaaacgaga tgcgcgggtg atcggtgggc
     2101 atgttgaagg tccacatcca ctcctcgatc ttgat
//
LOCUS       DAORNX010000499         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38558_length_2135_cov_5.629327, whole genome shotgun sequence.
ACCESSION   DAORNX010000499 DAORNX010000000
VERSION     DAORNX010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38558_length_2135_cov_5.629327"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>352
                     /locus_tag="PLO65_15765"
     CDS             <1..>352
                     /locus_tag="PLO65_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRD29758.1"
                     /translation="ATAGGAAAVEEAAAMGADWVMSAIVGAAGLAPTLAAARVGSVIA
                     LANKESLVCAGPVLLETARRAGGQVIPVDSEHSAIFQVLQPDCLQRVAKLILTASGGP
                     FRTWTRDQMAAATPE"
     assembly_gap    353..375
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <376..963
                     /locus_tag="PLO65_15770"
     CDS             <376..963
                     /locus_tag="PLO65_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRD29759.1"
                     /translation="SMGAKISIDSATMMNKGLEMIEASYLFSTPAERIDVLIHPQSVI
                     HSMVEYADGSTLAQLGPPDMRSPIACAFAWPDRLPWPAPRLDLAAMGQLTFEAPDPTR
                     FPAIGIARAALNAGGGAPAAMNAANEIAVAAFLDRKIGFLDIAPLVSDTLDRMNGVGD
                     LKGSVGDGALEGAMMVDASARRVAAEAVSQRPVFH"
     gene            986..>2135
                     /locus_tag="PLO65_15775"
     CDS             986..>2135
                     /locus_tag="PLO65_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRD29760.1"
                     /translation="MPDLVSWILPFLFVLTLVVTIHELGHYWAARAFGTVIDSFSLGF
                     GQAILKWRDKRGVQWRVGWIPLGGYVRFAGDENAASVPDHDDLEAMKAKILAHNDPAA
                     LQRFFHFKPVWQRAIITLAGPTANFVLAVGLFALLLSVFGEVRSVPRVTEVTPGSAAE
                     RAGFKPGDIITRADGREIHAFQDIIPLTVVRANIPIVFTVDRGGRELQLVATPAMKQV
                     TDPTGARQKIGVLGLSHLDRPEDLIRIRYNPVEAVAGGVQKTADILSTTLFYLGRLVT
                     GQVPADQLGGFIGIAKTSDAVAKAGAVGAPNPAAGVFGSFIALLQLSAILSVSVGFLN
                     LLPIPVLDGGHLVFYAYEAVARRPLAANVQAVGYRLGLALLLGFMLFAV"
BASE COUNT          299 a          719 c          724 g          370 t
ORIGIN      
        1 gccaccgccg gcggggcggc cgcggtcgag gaagcggccg ccatgggcgc cgactgggtg
       61 atgtcggcca tcgtcggggc ggccggcctg gcgccgacgc ttgccgcggc ccgcgtcggc
      121 tcggtgatcg cgctggccaa caaggaaagc ctggtctgcg ccggtccggt gctgctggag
      181 acggcgcggc gggccggtgg tcaggtgatc ccggtcgatt ccgaacattc ggcgatcttc
      241 caggtgcttc agccggactg cctgcagcgg gtggcgaagc tgatcctgac ggcctcgggc
      301 ggcccgttcc gcacctggac ccgcgaccag atggccgcgg ccacgccgga acnnnnnnnn
      361 nnnnnnnnnn nnnnnagcat gggggccaag atctcgatcg actccgccac catgatgaac
      421 aaggggctgg agatgatcga ggcctcctat ctcttctcca ccccggcgga acggatcgat
      481 gtcctcattc atccccagtc ggtgatccac agcatggtcg agtacgcgga cggctcgacc
      541 ctggcccagc tcgggccgcc cgacatgcgc agcccgatcg cctgcgcctt cgcctggccc
      601 gatcgtctgc cctggccggc gccccggctc gatctggcgg cgatgggcca gctgaccttc
      661 gaggcgcccg acccgacgcg ctttccggcc atcgggatcg cccgcgcggc gctcaacgcg
      721 ggcggcggcg cgccggcggc gatgaacgcc gccaacgaga tcgccgtggc ggctttcctt
      781 gaccgcaaga tcggctttct ggatattgcc ccgctggtct cggatacact cgatcgcatg
      841 aacggtgtgg gggatctgaa gggcagcgtg ggggacggcg cccttgaggg cgcgatgatg
      901 gtcgatgcga gcgcgcgccg ggtagcggcg gaagcggttt cgcaacgtcc agtcttccac
      961 tgaccgtagg ggagtaaatc ggccgatgcc cgacctggtg tcctggattc tgccgtttct
     1021 gttcgtgctc accctcgtgg tcacgattca tgaactgggg cactactggg cggcgcgggc
     1081 cttcggcacc gtgatcgaca gcttttcgct cggctttggc caggcgatcc tcaagtggcg
     1141 cgacaagcgc ggcgtgcaat ggcgcgtggg ctggatcccg ctgggcggct atgtccggtt
     1201 cgccggggac gagaacgcgg ccagcgtgcc cgatcacgac gacctggagg cgatgaaggc
     1261 caagatcctg gcccacaacg atccggccgc gctgcagcgc ttcttccatt tcaagcctgt
     1321 ctggcagcgg gccatcatca ccctggccgg tccgacggcg aacttcgttc tggcggtggg
     1381 gcttttcgcc ctgctgctga gcgtcttcgg cgaagtccgc agcgtgccgc gcgtcaccga
     1441 ggtgaccccg ggcagcgccg ccgaaagggc cgggttcaag ccgggcgaca tcatcacccg
     1501 ggccgacggg cgcgaaatcc acgccttcca ggacatcatt cccctgaccg tggtgcgggc
     1561 caatattccg atcgtcttca ccgttgaccg cggcggccgc gaacttcagc tggtcgccac
     1621 cccggcgatg aagcaggtga ccgacccgac cggcgcccgg cagaagatcg gcgtgctggg
     1681 cctgagccat ctcgaccggc ccgaggacct gatccgcatc cgctacaatc cggtcgaggc
     1741 cgtggccggc ggcgtgcaga agacggcgga catcctgtcg acgaccctct tctacctcgg
     1801 ccgtctggtc accggccagg tgccggccga tcagctgggc ggcttcatcg gcatcgccaa
     1861 gacctccgat gccgtggcca aggcgggcgc cgtgggagcg ccgaacccgg cggccggcgt
     1921 gttcggctcc ttcatcgccc tgttgcagct ttcggcgatt ctctcggtca gcgtgggctt
     1981 cctcaatctg ctgccgatcc cggttctcga tggcgggcac ctggtattct acgcctacga
     2041 ggccgttgcc cgcaggccac tggcggcaaa tgtccaggcg gtcggctatc gccttggtct
     2101 tgccttgctg ttgggattca tgctgttcgc ggtct
//
LOCUS       DAORNX010000500         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38661_length_2130_cov_9.884337, whole genome shotgun sequence.
ACCESSION   DAORNX010000500 DAORNX010000000
VERSION     DAORNX010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38661_length_2130_cov_9.884337"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLO65_15780"
     CDS             <1..358
                     /locus_tag="PLO65_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HRD29761.1"
                     /translation="GYGLARPREALAARGLAAAEAGGGGLGAARGFGAMLLLAHAGTA
                     GLLGYYPSVGASMALALALLWGGSAVGRLASGILDGHGDARSIQLVLFEVLMGLTLSL
                     PFWASRQLTAGPVFNI"
     gene            458..886
                     /locus_tag="PLO65_15785"
     CDS             458..886
                     /locus_tag="PLO65_15785"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HRD29762.1"
                     /translation="MKRMGLGLTALVIGTGLAGAAIAADVTVNLTGVQPRGGRMLVSL
                     QTADEFMKPEGRYGAIGAGDVTGDETLVVRDVPPGEYAVMVLHDADGDMRMARAPDGK
                     PGEGWGMSGQWTRGQRPGFAEVKITVPADGTAVAVPLVYP"
     gene            complement(977..>2130)
                     /locus_tag="PLO65_15790"
     CDS             complement(977..>2130)
                     /locus_tag="PLO65_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD29763.1"
                     /translation="KEKAKAAGLWNFFMPPHSGQTHVDDTFQFEGIQLTNLEYSMIAE
                     QMGKIGFASEVFNCSAPDTGNMEVLMRYGTLEQKERWLRPLMNGEIRSAFLMTEPAVA
                     SSDATNIETEIRRDGDEYVINGRKWWSSGVGDPRCKVAIVMGKTDPDNASRHQQQSQI
                     LVPMDAPGIEIVRMLPVFGYDDAPHGHAEVILKDVRVKVEDALLLGEGRGFEIAQGRL
                     GPGRIHHCMRTIGTAEVALEKMCKRLMSRKAFGKYLSDHSVWEQRVAEARIDIEMCRL
                     LCLKAADMMDKAGNKSARLEIAMIKVAAPRIALKIIDDAIQAHGGAGVTTDFGLASAY
                     AHQRTLRLADGPDEVHCRTIARDEFGKYGDLKMQQKNERDKSLEVAGIR"
BASE COUNT          297 a          687 c          763 g          383 t
ORIGIN      
        1 cggctacggc ctggcgaggc cgcgggaagc gctggccgcc cgcggacttg cagcggctga
       61 agcggggggc ggcggcctcg gggcagcgcg cggcttcggg gccatgttgc tgctggcgca
      121 cgcggggacg gccggcctgc tcggctacta tccctccgtc ggggcgagca tggccctggc
      181 cctggccctg ctgtggggcg gttcggcggt cgggcggctg gcgtccggca ttctcgacgg
      241 ccacggcgac gcccggtcga tccagctggt cctgttcgag gtcctgatgg ggctgaccct
      301 gtccctgccc ttctgggcca gccggcagct gacggccggc ccggtgttca acatctaggc
      361 tcgcccgggg aggcgggcgc acatgaccaa atcgtaacgt gacgacaatg tgctttgttt
      421 tataaagcac attgtctcaa caagaggagc gacgcttatg aaacgcatgg gattgggcct
      481 gacggcgctg gtgatcggca cgggcctggc gggcgcggcg attgccgcgg acgttaccgt
      541 caacttgacg ggcgttcagc ccaggggcgg ccggatgctg gtgtccctgc agacggccga
      601 cgagttcatg aagcccgagg gccgctacgg cgcgatcggc gcaggcgacg tcaccggcga
      661 cgaaacgctc gtcgtgcggg acgtcccgcc cggggagtac gcggtgatgg tcctgcacga
      721 cgccgatggg gacatgcgca tggcccgggc gcccgacggc aaacctgggg aaggctgggg
      781 gatgagcgga cagtggaccc gcggccagcg tccgggcttc gccgaggtga agatcaccgt
      841 gcccgccgac ggaacggcgg tcgccgtgcc gctggtctat ccgtgaaccc gcgcggggac
      901 atgcgcttca acgcatgtcc ccctgtccgc ggaagacagg ggacacgtac cggatgcgga
      961 catgtccccg tgggtcctac cggatgccgg cgacttccag cgatttgtcc cgctcgttct
     1021 tctgctgcat cttcagatcg ccgtacttgc cgaactcgtc gcgggcgatg gtgcggcagt
     1081 ggacctcgtc cgggccgtcc gccaggcgca gggtgcgctg gtgggcgtag gccgaggcca
     1141 ggccgaagtc ggtggtcacg ccggcgccgc cgtgggcctg gatggcgtcg tcgatgatct
     1201 tcagggcgat ccgcggcgcc gccaccttga tcatggcgat ctccagacgg gccgacttgt
     1261 tgccggcctt gtccatcatg tcggccgcct tgaggcagag caggcggcac atctcgatgt
     1321 cgatgcgggc ctcggcgacg cgctgttccc agaccgagtg atcgctgagg tacttgccga
     1381 acgccttgcg gctcatcagg cgcttgcaca tcttttccag cgcgacttcc gccgtgccga
     1441 tggtgcgcat gcagtggtgg atgcggcccg gtccgaggcg gccctgggcg atctcgaagc
     1501 cgcggccctc gccgagcagc agggcgtctt cgaccttcac ccggacatcc ttgaggatga
     1561 cctcggcgtg gccgtgcggc gcgtcgtcat agccgaacac cggcagcatg cggacgatct
     1621 cgatacccgg cgcgtccatc ggcacgagga tctgcgactg ctgctggtgg cggctggcgt
     1681 tgtccgggtc ggtcttgccc atgacgatgg ccaccttgca gcgcgggtcg cccacgcccg
     1741 aggaccacca cttgcggccg ttgatgacgt actcgtcgcc gtcgcggcgg atttcggttt
     1801 cgatgtttgt ggcgtcggaa ctggccacgg ccggctcggt catcaggaag gcgctgcgga
     1861 tctcgccgtt catcagcggg cgcagccagc gctccttctg ctccagcgtg ccgtagcgca
     1921 tcagcacttc catgttgccg gtgtccggcg cggagcagtt gaacacctcg gacgcgaagc
     1981 cgatcttgcc catctgctcg gcgatcatgc tgtattcgag gttggtcagc tggatgcctt
     2041 cgaactggaa ggtgtcgtcc acatgggtct ggccgctgtg cggcggcatg aagaagttcc
     2101 acaggccggc ggccttggcc ttttccttca
//
LOCUS       DAORNX010000501         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38662_length_2130_cov_8.747952, whole genome shotgun sequence.
ACCESSION   DAORNX010000501 DAORNX010000000
VERSION     DAORNX010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38662_length_2130_cov_8.747952"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1778
                     /locus_tag="PLO65_15795"
     CDS             81..1778
                     /locus_tag="PLO65_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HRD29764.1"
                     /translation="MARQLRRSPEAATSLGIDKGDMAWTKSQLSDFSLAALKENGEYN
                     AQELKSLRTVDRSKLSGMDAASYDTVEFVLANGDEFTEKFAYNGAYGGAPYVISQLTG
                     SYQQVPDFLDSQHVIETKADADAYLSRVEAFGRLLDQEVEVAEHDAALGVVPADFTID
                     KALIQFKSMLGVAPDASPLVLSVARRAKEKGITGDYAGQAEKLYAEKIRPALARQAAL
                     LEGWRPKAVHDAGVWRLPKGEDYYLTSLENYTTSRITPDEVHQTGLDMVASLGAQCDK
                     LMRKAGYASGTVGERFRQMYSDPKQHYPNTDEGKVQLIADLNDKVKAITPLLPKYFGQ
                     LPKAPLEIRRVPKAIEAGAPGGYYNSPSLDGSRPGIYWINLRDSSEQPRFTLSTLTYH
                     EAIPGHHLQLSLSNEAGDLPLIRKMIGFSGYSEGWALYAEQLAVEMGMYEHDPVGQIG
                     MLHDAMFRAVRLVVDSGMHHKRWSREQAVKYYVDNIGDQEASAITEIERYVVWPGQAC
                     SYMVGKIEFLRLRAKAQKALGRRFDIRKFHDACLLSGGVPLTVLERVVDGYIAGAKA"
     gene            complement(1891..>2130)
                     /locus_tag="PLO65_15800"
     CDS             complement(1891..>2130)
                     /locus_tag="PLO65_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRD29765.1"
                     /translation="LLPVPGLDGYGVIRPFLPTALQARLAPIEPLAILALIAMVFFLP
                     GASGLLWGAALGLTDLTGVDTDQIGRGLAAFRFWR"
BASE COUNT          377 a          746 c          720 g          287 t
ORIGIN      
        1 acgccttcgc cgctgtcgcc ggcgccggcg cgagcgaccc ggccgaggtc gccaaggcca
       61 ccgccatcta tgacgccctg gtggcccgcc agctgcgccg ctcgccggaa gcggcaacca
      121 gcctcggcat cgacaagggc gacatggcct ggacaaagtc gcagctgtcg gacttctcgc
      181 tcgcggccct caaggagaac ggcgaataca acgcccagga gctgaagagc ctgcgcaccg
      241 tcgaccgcag caagctctcc ggcatggacg cggccagcta cgacacggtc gagttcgtcc
      301 tggccaacgg cgacgagttc accgagaagt tcgcctacaa cggcgcctat ggcggcgcgc
      361 cctatgtcat ctcgcagctg accgggtcct accagcaggt ccccgacttc ctcgacagcc
      421 agcacgtgat cgagaccaag gccgacgccg acgcctatct gtcgcgggtc gaggccttcg
      481 gccggctgct cgaccaggag gtcgaggtcg ccgagcatga cgccgccctg ggcgtcgttc
      541 cggccgactt caccatcgac aaggcgctga tccagttcaa gagcatgctc ggcgtggcgc
      601 cggacgcctc gccgctggtg ctatccgtgg cccgccgggc caaggagaag ggcatcaccg
      661 gcgactacgc cggccaggcc gagaagctct acgccgagaa gatccgcccg gccctggccc
      721 gccaggcggc cctgctcgag ggctggcggc cgaaggccgt gcacgacgcc ggtgtctggc
      781 gcctgcccaa gggcgaggac tactacctga cctcgctgga gaactacacg acctcgcgga
      841 tcacccccga cgaggtgcac cagaccggcc tcgacatggt cgccagcctc ggcgcccagt
      901 gcgacaagct gatgcgcaag gcgggctacg ccagcggcac ggtcggcgag cgcttccgcc
      961 agatgtactc ggacccgaag cagcactatc cgaacacgga cgaaggcaag gtccagctga
     1021 tcgccgacct gaacgacaag gtgaaggcga tcaccccgct gctgccgaaa tacttcggcc
     1081 agctgcccaa ggccccgctg gagatccgcc gcgtgcccaa ggccatcgag gccggcgccc
     1141 cgggcggcta ctacaattcg cccagcctcg acggctcgcg gccgggcatc tactggatca
     1201 atctgcgcga cagcagcgag cagccgcggt tcacgctgtc gaccctgacc tatcacgagg
     1261 cgattccggg ccaccacctg cagctgtcgc tgagcaacga ggccggcgat ctgccgctga
     1321 tccgcaagat gatcggcttc tcgggctaca gcgagggctg ggcgctctac gccgagcagc
     1381 tggcggtgga gatgggcatg tacgagcatg acccggtcgg ccagatcggc atgctgcacg
     1441 acgcgatgtt ccgcgccgtg cggctggtcg tcgacagcgg catgcaccac aagcgctgga
     1501 gccgcgagca ggcggtcaaa tactacgtcg acaatatcgg cgaccaggag gccagcgcga
     1561 tcaccgagat cgagcgctac gtcgtctggc cgggccaggc ctgcagctac atggtcggca
     1621 agatcgagtt cctgcgcctg cgcgccaagg cccagaaggc gctcggccgc aggttcgaca
     1681 tccgcaagtt ccacgacgcc tgtctgctgt cgggcggggt gccgctgacg gtgctcgagc
     1741 gggtggtcga cggctatatc gccggcgcga aggcctaggg tccaggcaag aggggccagg
     1801 ggctaggggc tgggatttgg gataggcagc cggccggcgt gcgaactttc tagtccctag
     1861 tccctggttc ctggtccctg ttccctggcc ctaccgccag aaccggaacg ccgccaggcc
     1921 ccggccgatc tggtcggtgt cgacgccggt caggtcggtc aggcccagcg cggcgcccca
     1981 cagcaggccc gaggcgccgg gcaggaagaa caccatggcg atgagggcga ggatggccag
     2041 cggctcgatc ggggccagcc gcgcctgcag cgccgtcggc aggaacggcc ggatcacgcc
     2101 gtagccgtcg aggcccggaa ccggcagcag
//
LOCUS       DAORNX010000502         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38704_length_2128_cov_6.000965, whole genome shotgun sequence.
ACCESSION   DAORNX010000502 DAORNX010000000
VERSION     DAORNX010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38704_length_2128_cov_6.000965"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLO65_15805"
     CDS             <1..1393
                     /locus_tag="PLO65_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HRD29766.1"
                     /translation="AIRRGAAGRAADVLAAEGRIVAAIQKRGYADAEPGVREVIVDHA
                     DHTVRPTFRIVAGDRVIMDGLTVQPAGRTRPDWVVRLAPWKAGDVYDPEDLAELERRL
                     LDTGVYDSVSVSLQPADKAAADGRRPVLVGLTDRPRRTLEVSAGYSTSEGAGLDLRRT
                     RFNQLGRADTTTYTLRLAELEQRFEARLALPHWRRPQQTLTLVAGVYNALTDAYDAAG
                     VMATGDITRRFGKTSFVTAGLALDASRSAEKTQVNNATIRGAERDILTASVLAAMSLD
                     RSDDPLDPTRGWRVEARAEPTAAVGDLQALYLKVQGQGSAYVSLDEDSRTVLAARLKI
                     GSIFGAVLDDVSAPRRFYAGGGGSVRGYAWQAIGPRLADNTPQGGLSLFEASFEARRR
                     LTDRWGVVAFVDAGAVGAESYPQGDDFSVGAGLGVRYDLGFGPIRADIAVPLDKREGD
                     PAFQIYISIGQAF"
     gene            1390..>2128
                     /locus_tag="PLO65_15810"
     CDS             1390..>2128
                     /locus_tag="PLO65_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HRD29767.1"
                     /translation="MSESAPSPQTPTRRRLLPGGGPGWVLLVSLLAVLLVGGVALVTR
                     YGVNTAPGLLFIEARASGLKLGRYGRLKIEGLHGDLWRDFTVRRLTISDEKGVWLDAD
                     NVVLTWRYHELLFRRFHADRLAAGRVRILRRPTLTPKTTSRNLPVSVRIDKARMVVET
                     LPAFSYRPGLFDVVGSLDIVRKGPVHVRLGAGSRLHAGDRIGLAIDSGKGAPLVVIAD
                     AEEVEGGAIAGSLGLAADKPFLLKVRAG"
BASE COUNT          291 a          766 c          753 g          318 t
ORIGIN      
        1 ggccatccgc cgcggcgcgg ccggcagggc cgccgatgtg ctcgccgccg agggccgcat
       61 cgtcgccgcc atccagaagc gcggctacgc cgacgccgaa ccgggcgtgc gcgaggtgat
      121 cgtcgaccac gccgaccata cggtgcggcc gaccttccgc atcgtcgccg gcgatcgggt
      181 gataatggac gggctgaccg tccagccggc cggccggacc cggcctgact gggttgtccg
      241 gctggcgccg tggaaggccg gcgacgtcta tgatccggag gacctggccg agctcgaacg
      301 ccggctgctc gacaccgggg tctatgattc cgtctcggtg tcgctgcagc cggccgacaa
      361 ggccgctgcc gatggccgtc ggccggtgct ggtgggcctg accgatcgcc cgcggcggac
      421 cctcgaggtc agcgccggct attcgaccag cgagggcgcg ggcctcgacc tgcgccggac
      481 ccggttcaac cagctgggcc gggcggacac caccacctac accctgcgcc tggccgaact
      541 cgaacagcgg ttcgaggccc ggctggccct gccgcactgg cgccggccgc agcagaccct
      601 gaccctggtc gccggggtct acaacgccct gaccgacgcc tatgacgcgg ccggcgtgat
      661 ggccaccggc gacatcaccc ggcggttcgg caaaacctcg ttcgtcaccg ccggcctggc
      721 gctcgacgcc agccgcagcg cggaaaagac gcaggtcaac aacgccacca tccggggcgc
      781 cgagcgcgac atcctgaccg cttcggtgct ggcggcgatg tcgctggacc gttccgacga
      841 tcctctcgac ccgactcggg gctggcgggt cgaggcgagg gccgagccaa ccgccgcggt
      901 cggcgacctg caggcgctgt atctcaaggt tcaggggcag ggttcggcct acgtctcgct
      961 cgacgaggac tcccgcaccg tgctggccgc ccggctcaag atcggctcga tcttcggggc
     1021 ggtcctcgac gacgtctcgg cgccgcgacg gttctatgcc ggcggcggcg ggtcggtccg
     1081 cggctacgcc tggcaggcca tcgggccgcg actggccgac aatacgcccc agggcggcct
     1141 gtcactgttc gaggcttcgt tcgaggcgcg gcgccgcctg accgaccgct ggggtgtggt
     1201 ggcgttcgtc gacgccgggg cggtcggggc ggaatcctac ccacagggcg acgacttcag
     1261 cgtcggagcc ggactggggg tacggtacga cctcggtttc ggaccgatcc gcgccgacat
     1321 cgccgtgccc ctcgacaagc gggagggcga cccggcgttc cagatctaca tcagcatcgg
     1381 acaggcgttt tgagcgagtc cgccccatcg ccgcagaccc ccacgcgtcg acggcttctg
     1441 ccgggcggcg gaccgggctg ggtcctgctc gtctcgttgc tggcggtact ccttgtcggc
     1501 ggggtcgcac tggtcacccg gtacggcgtc aacaccgcgc cgggcctact gttcatcgag
     1561 gcccgcgcca gcggcctcaa gctcggccgc tacggacggc tgaagatcga agggctgcac
     1621 ggcgacctgt ggcgcgactt caccgtccgc cgcctgacca tcagcgacga gaagggtgtc
     1681 tggctcgacg ctgacaatgt cgtgctgacg tggcgctacc acgagctgct ttttcggcgc
     1741 tttcatgccg atcgcctcgc ggcggggcga gttcgcatcc tgcgtcgtcc gacgctgaca
     1801 cccaagacca cctcgcgaaa cctccccgtc tcggtgcgga tcgacaaggc ccgaatggtc
     1861 gtcgagaccc tgccggcctt cagctatcgg cccggcctgt ttgacgtcgt gggttcgctc
     1921 gacatcgttc gcaaagggcc ggtccacgtg cgcctgggcg ccggcagccg gctgcacgcc
     1981 ggcgaccgca ttggcctggc catcgacagt ggcaagggcg cgccgctggt cgtgatcgcc
     2041 gacgccgagg aggtcgaggg cggggccatc gccggctcgc tcgggctggc ggccgacaag
     2101 cccttcctgc tcaaggtgcg ggcgggcg
//
LOCUS       DAORNX010000503         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38816_length_2123_cov_4.482108, whole genome shotgun sequence.
ACCESSION   DAORNX010000503 DAORNX010000000
VERSION     DAORNX010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38816_length_2123_cov_4.482108"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1084)
                     /gene="rlmB"
                     /locus_tag="PLO65_15815"
     CDS             complement(278..1084)
                     /gene="rlmB"
                     /locus_tag="PLO65_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRD29768.1"
                     /translation="MSSFDERNDRKKGFHKRPEKGGKPRPQRVSASDAKDWLWGVHAV
                     EAALSNPARPAPRRLLVTEDRARTLSRRFPNLRVETADAHEIGRHLPQGAVHQGMALR
                     IDPPEPLSIEELGAPAEGVLVMLDQLTDPQNVGAIFRSAAAFGAKGVILQDRHAPALS
                     GALAKAAVGAVDRLPHALVVNLSRALEQLSEAGWRIVGLDGASDTALDAALDGSPTVL
                     VMGSEGEGLRRLVSEHCDVLARIPMPGGFESLNVSNAAAIALYECARNRA"
     gene            1172..1255
                     /locus_tag="PLO65_15820"
     tRNA            1172..1255
                     /locus_tag="PLO65_15820"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1206..1208,aa:Tyr,seq:gta)
     gene            1333..1406
                     /locus_tag="PLO65_15825"
     tRNA            1333..1406
                     /locus_tag="PLO65_15825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1365..1367,aa:Gly,seq:tcc)
     gene            1451..>2123
                     /locus_tag="PLO65_15830"
     CDS             1451..>2123
                     /locus_tag="PLO65_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD29769.1"
                     /translation="MAKEKFERNKPHCNIGTIGHVDHGKTTLTAAITITLAKTGGATA
                     KNYADIDAAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSS
                     YNFPGDDIPIVKGSALAAVEGRDAPIGEDAILALMTAVDTYIPQPDRPVDLPFLMPVE
                     DVFSIS"
BASE COUNT          400 a          673 c          727 g          323 t
ORIGIN      
        1 gtggagccgc ggtcatatcc ggcgggcggc ggaccggcat agccgtcgtc cgggtggccg
       61 ccgccacacc ccgttagcgc gagaaaagcg gcgcccgagg cggcgcaggc ccccaaacgc
      121 accaagcctt tcaacatcgg aacccccgat cccatgccga agaaaacggg tctgtttcga
      181 cccgccatca aacaaggcgc gaagagaaac acgccttcgc gacgaaatca aaacggttaa
      241 ttcagattcg cgtaacctgt tcgcctgacg cgggaattca ggccctattt cgggcgcatt
      301 cgtacagggc gatggccgcc gcgttcgaca cgttgaggct ttcgaagccg ccgggcatcg
      361 gaatcctggc caggacgtcg cagtgctcgg aaaccagtcg gcgcaagccc tcgccttcgg
      421 agcccatgac cagaaccgtc ggcgagccgt ccagggcggc gtccagcgcc gtgtccgagg
      481 cgccgtcgag accgacgatc ctccagccgg cctccgacag ctgttcgagg gcgcgggaga
      541 ggttaacgac gagggcgtgg ggcagccggt cgacggcccc gaccgccgcc ttggcgaggg
      601 cgccggagag ggcgggggcg tggcggtcct gcaggatgac gcccttcgcc ccgaaggcgg
      661 cggccgagcg gaagatggcg ccgacattct gcgggtcggt cagctggtcg agcatgacca
      721 ggacgccttc cgcgggcgct cccagctctt ctatggagag tggctcgggc gggtcgatgc
      781 gcagggccat cccctggtgc acggcgccct ggggcagatg gcggccgatt tcgtgcgcat
      841 cggcggtctc gacccgcagg ttcgggaatc gacgactcag cgtccgggcg cgatcctcgg
      901 tgaccaggag tcggcgcggc gcgggccggg ccgggttcga caatgccgct tcgacggcgt
      961 ggacgcccca cagccagtcc ttcgcatcgc tcgcggaaac cctttgcggg cgaggctttc
     1021 cgcctttttc aggccgcttg tgaaagcctt ttttccggtc gttgcgttca tcaaacgagg
     1081 acactataag acgcgctccg atttggggcc ccaggccgct tcgcttaacc gcgaaggggc
     1141 tggccgccaa ggaaagagca ccatcgcgcg agggggaatg tcccgagcgg caaagggggc
     1201 ggactgtaaa tccgctgcga aagcttcgta ggttcgagtc ctacttcccc caccacgcac
     1261 gatgatggtt cttctccccg gcagacgcgg gacctcgaac tgaacaggag acgacggcga
     1321 cccagccggc aggcgggtat agcacaatgg tagtgcagca gccttccaag ctgaggatgc
     1381 cggttcgatc ccggctaccc gctccagtcg cctttcacag aaccccgccg ccagccccaa
     1441 ggtagagaat atggccaagg aaaaattcga acgtaacaag ccgcactgca acatcggcac
     1501 gattggtcac gttgaccatg gcaagacgac gctgacggcg gcgatcacga tcacgctggc
     1561 gaagaccggc ggtgcgacgg cgaagaacta cgcggacatt gacgcggcgc cggaagagaa
     1621 ggcgcggggc atcacgatca acacggcgca cgtggaatat gagacggcga accgtcacta
     1681 cgcgcacgtg gactgcccgg gccacgccga ctacgtgaag aacatgatca cgggcgcggc
     1741 gcagatggac ggcgcgatcc tggtggtttc ggcggcggac ggtccgatgc cgcagacgcg
     1801 cgagcacatc ctgctggccc gtcaggtggg cgtgccggcg ctggtggtgt tcatgaacaa
     1861 ggtcgacatg gtcgacgacg ccgagctgct ggacctggtg gagatggaag tgcgcgagct
     1921 gctgagcagc tacaacttcc cgggcgacga cattccgatc gtcaagggct cggccctggc
     1981 ggcggttgaa ggtcgtgacg cgccgatcgg cgaggacgcg atcctggcgc tgatgacggc
     2041 ggtggacacc tacatcccgc agccggaccg tccggtggac ctgccgttcc tgatgccggt
     2101 ggaagacgtg ttctcgatct cgg
//
LOCUS       DAORNX010000504         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38842_length_2122_cov_5.629415, whole genome shotgun sequence.
ACCESSION   DAORNX010000504 DAORNX010000000
VERSION     DAORNX010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38842_length_2122_cov_5.629415"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLO65_15835"
     CDS             <1..448
                     /locus_tag="PLO65_15835"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRD29770.1"
                     /translation="PLAVHDEGATAALRLGPDEWLVLRVHDPAGAGPWPPGPPPEEPV
                     SVVDVSDRQVAILISGVAAADLLNAGAPLDLSPAAFPVGRVARTVFGRVEVVIWRRSA
                     ESFHLEVWRSMAGYVWRILALAHADIISDPASLASRPGAAGPADNS"
     gene            462..890
                     /locus_tag="PLO65_15840"
     CDS             462..890
                     /locus_tag="PLO65_15840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRD29771.1"
                     /translation="MHETARVHFGVAHPWLCDSMGHLTSRHYVAMFDDASYHFFRLVF
                     GYDAAAADWAGLGFADVRQVIEYRSEVSSGDLVHIDGEIARLGDKSITARFTMRRSSD
                     DTEVATLEATTVFFDLVARRARSLTEDMRRRGARWTAPVA"
     gene            complement(915..989)
                     /locus_tag="PLO65_15845"
     tRNA            complement(915..989)
                     /locus_tag="PLO65_15845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Gly,seq:ccc)
     gene            1100..1699
                     /locus_tag="PLO65_15850"
     CDS             1100..1699
                     /locus_tag="PLO65_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRD29772.1"
                     /translation="MADGAHGSGERAGFHSMVESTAEDWARIAAASAEYGRELPGRVI
                     AHLQLLKGDCGGFVVDRLEHSLQTATRAFRDGRDEEYVVCALLHDIGDVLCPRNHPDI
                     AAAMVFPYVSEANHFMVAQHGIFQGYYFFHHLGLDRDMREQFRGHPHFEYTAQFCHLY
                     DQSAFDPAYESMPLEAFEPMLQRVMAVPKRSIYLRPAAE"
     gene            complement(1760..>2122)
                     /locus_tag="PLO65_15855"
     CDS             complement(1760..>2122)
                     /locus_tag="PLO65_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD29773.1"
                     /translation="DAAFAASQPRVVAGLAKTEPARAEALAWSLASDRGVMARATYDV
                     MTTDLRGDLAAIRIPVTVAYARDPAMGFFYGVVDQVYATNYAGLAGVRLRPVDGAFHH
                     LMLDQPALFAAEVETFLK"
BASE COUNT          307 a          770 c          725 g          320 t
ORIGIN      
        1 cccgttggct gttcatgacg agggggcgac ggccgccctg cggctcgggc cggacgaatg
       61 gctggtgctc cgggtccacg accccgccgg cgccggtcca tggccgccgg ggccgcctcc
      121 agaggagccc gtctcggtcg tcgacgtctc cgaccggcag gtcgcgatcc tcatcagtgg
      181 cgtcgcggcc gccgacctgc tgaacgccgg cgctccgctg gacctgtccc ccgcggcctt
      241 ccccgtcggc cgcgtggcca gaaccgtgtt cggccgggtg gaggtcgtga tctggcggcg
      301 gtccgccgaa agcttccacc tcgaggtctg gcgatcgatg gccggctacg tctggcggat
      361 cctcgccctg gcccacgccg acatcatcag cgaccccgcc agcctggcgt cccggccagg
      421 cgccgccggc cctgccgaca actcctagaa aggcgaccgc catgcacgag acagcccggg
      481 tccatttcgg cgtcgcgcac ccctggctat gcgattccat gggccatctg accagccgcc
      541 actatgtggc gatgttcgat gacgccagct accacttctt ccgcctggtc ttcggctacg
      601 acgccgcggc cgccgactgg gccggcctcg gcttcgccga cgtccgccag gtgatcgagt
      661 accgctcgga agtgtcgtcg ggcgatcttg tgcatatcga tggcgagatc gcccggctgg
      721 gcgacaagtc gatcaccgcc cggttcacca tgcggcggtc gtcggacgat acggaggtgg
      781 cgaccctcga ggcgacgacc gtgttcttcg acctcgtcgc ccgccgcgcc cgctccctga
      841 ccgaggacat gcgccggaga ggcgcgaggt ggacggcgcc ggtggcctga accggcgccg
      901 ggcgccggga cccctggagc ggccgggggg aatcgaaccc ccgacattca gcttgggaag
      961 ctgacgttct acctctgaac tacggccgcg ctctgggagc cgggcggaac atagccaaac
     1021 gcgcggcgcc cgcaagcggc gttgacgccc gccctgccct cccgcacgat cggctcgaac
     1081 agacgttggg agacaggcca tggctgacgg cgcgcacggg tccggggaac gggccggttt
     1141 ccactcgatg gtcgagagca cggccgagga ctgggccagg atcgccgcgg cctccgccga
     1201 gtacggccgc gagctgccgg ggcgggtgat cgcccatctg cagctgctca agggcgactg
     1261 cggcggtttc gtcgtcgacc ggctcgagca cagcctgcag accgccaccc gcgccttccg
     1321 tgacgggcgc gacgaggaat atgtcgtctg cgccctgctg cacgacatcg gcgacgtgct
     1381 gtgcccgcgc aaccacccgg acatcgccgc ggcgatggtc ttcccctacg tgtccgaggc
     1441 caaccatttc atggtcgccc agcacggcat cttccagggc tactacttct tccatcacct
     1501 ggggctcgac cgcgacatgc gcgagcagtt ccgcggccat ccgcacttcg aatacacggc
     1561 ccagttctgc cacctgtacg atcagtcggc gttcgacccg gcctatgagt cgatgccgct
     1621 ggaggcgttc gagccgatgc tgcagcgggt gatggccgtg cccaagcggt cgatctacct
     1681 gcggccggcg gcggaataga gccgctcgat cgtcgtcgtc ccggacggcc gccaggccgt
     1741 tccgggagga tcgccgagcc tacttcaaga acgtctccac ctcggccgcg aacagcgccg
     1801 gctggtcgag catcagatgg tgaaacgccc cgtcgacggg ccgcagccgg acgccggcca
     1861 ggccggcgta gttggtcgca tagacctggt cgaccacgcc gtagaagaag cccatggccg
     1921 gatcgcgggc gtaggcgacc gtcacgggaa tcctgatcgc ggccagatcg ccgcgcaggt
     1981 cggtggtcat cacgtcgtag gtcgcccggg ccatcacgcc gcggtcgctg gccagcgacc
     2041 aggcgagggc ctcggcccgg gccggctcgg tcttcgccag gcccgccacg acccgcggct
     2101 ggctggcggc gaaggccgcg tc
//
LOCUS       DAORNX010000505         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_38876_length_2121_cov_5.468054, whole genome shotgun sequence.
ACCESSION   DAORNX010000505 DAORNX010000000
VERSION     DAORNX010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38876_length_2121_cov_5.468054"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="mtaB"
                     /locus_tag="PLO65_15860"
     CDS             <1..1223
                     /gene="mtaB"
                     /locus_tag="PLO65_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRD29774.1"
                     /translation="TFGCRLNAYESEALRARAQADGVENAVVFNTCAVTNEAVRQARQ
                     AIRKARRERPGARLIVTGCAAQIDPQAFAAMPEVDLVLGNAEKAQPGALADTSARVRV
                     NDIMSVRETAGHLIDGLRDRARAFVEVQNGCDHRCTFCIIPFGRGNSRSAAAGEVVEQ
                     VRRLTAEGYREVVLTGVDVTSWGADLPGQPTLGQLVSRILKLVPDLPRLRLSSIDAAE
                     IDPDLMRCLAEEPRLMPYLHLSLQAGDDLVLKRMKRRHLRADALKLIADVRAVRPDTA
                     FGADLIAGFPTESDAAFDNTLALVEEAGLSFLHVFPYSARPGTPAAKMPPVKGTVIKD
                     RARRLRAAGEQALQRHLQRQVGRTLNGLVEKAGVARADDFTEIAFAGEAPVGEIAPMR
                     VTGVSGRTVTAKLL"
     gene            1354..2055
                     /locus_tag="PLO65_15865"
     CDS             1354..2055
                     /locus_tag="PLO65_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29775.1"
                     /translation="MRANSSGRLWAILTTMTGLATLIISIAFSRLPAVRTARACMTHG
                     DVIRFELARSVESLTAIFGPAAAPCRGLVIAAMDAINHFDIAAFIPTYSAFAVSSAVW
                     LVGRLLTPLTLIAIAAALVAFGADLIETITLLEMTNDLSTADSLALVASAAAWTKFTA
                     LTVHALVLSAACLVVTPRRWGLSLLLLAPLVGTSVAISDVARQAHMLSLGLGVAWGAL
                     VLTALREALRRRRPA"
BASE COUNT          302 a          774 c          749 g          296 t
ORIGIN      
        1 tcaccttcgg gtgccggctg aacgcctatg agagcgaggc cctgcgcgcg cgggcacagg
       61 cggacggggt cgagaacgcg gtggtgttca acacctgcgc cgtcaccaac gaggccgtcc
      121 gccaggcgcg gcaggcgatc cgcaaggcgc ggcgcgaacg gccgggcgcc cggctgatcg
      181 tcaccggctg cgccgcccag atagacccgc aggccttcgc cgccatgccc gaggtcgacc
      241 tggtgctggg caacgccgaa aaggcgcagc cgggcgcgct ggccgatacg tcggcgcggg
      301 tgcgggtcaa cgacatcatg tccgtacggg agaccgccgg ccatctgatc gacggcctgc
      361 gcgaccgggc gcgcgccttc gtcgaggtgc agaacggctg cgaccaccgc tgcaccttct
      421 gcatcatccc gttcggccgg ggcaattcgc gcagcgccgc cgccggcgag gtggtcgaac
      481 aggtccgccg gctgaccgcc gagggctatc gcgaggtggt gctgacgggc gtcgacgtca
      541 ccagctgggg cgccgacctg cccggccagc cgacgctggg ccagctggtc tcgcgcattc
      601 tgaagctggt cccggacctg ccgcgcctgc ggctgagctc gatcgacgcg gccgagatcg
      661 acccggacct aatgcggtgc ctggccgagg agccgcggct gatgccctac ctgcacctca
      721 gcctgcaggc cggggacgac ctggtcctca agcggatgaa gcgccgccac ctgcgcgccg
      781 acgccctcaa gctgatcgcg gacgtgcgcg cggtgcggcc cgacaccgcc ttcggcgccg
      841 acctgatcgc cggcttcccg accgagagcg acgcggcgtt cgacaacacc ctggccctgg
      901 tcgaggaagc gggcctgagc ttcctgcacg tcttccccta cagcgcccgc ccgggcaccc
      961 cggcggcgaa gatgccgccg gtgaagggca cagtgatcaa ggaccgcgcc cggcgactgc
     1021 gcgcggccgg cgagcaggcc ttgcagcggc acctgcagcg ccaggtcgga cggaccctga
     1081 acgggctcgt cgagaaggcc ggcgtggccc gtgcggacga cttcaccgag atcgccttcg
     1141 cgggcgaggc gccggtgggc gagatcgcgc cgatgcgggt gacgggggtg agcggccgga
     1201 cggtgacggc caagcttctc tgacctgccc ctcccggagg gagagggaga cgccggtggc
     1261 ggatcggccg cgcggcgccc cgcgacaatt gaatccagtg ttctccgaaa cggggttcgc
     1321 cataatccgg gcggacgacc cgggggggac gccttgcgcg ccaacagcag cggacgcctc
     1381 tgggcgatcc tgacgacgat gaccggactg gcgaccctga tcatcagtat cgccttcagt
     1441 cgcctgcccg ccgtgcgcac cgcgcgcgcc tgcatgaccc acggcgatgt aatccgtttc
     1501 gaactggccc gctcggtcga aagcctgacc gcgatcttcg gccccgccgc cgcgccctgc
     1561 cgcggactgg tcatcgcggc gatggacgcg atcaaccatt tcgacatcgc ggccttcatc
     1621 ccgacctaca gcgccttcgc ggtatcctcg gccgtgtggc tggtcgggcg gctgttgacg
     1681 cccctgaccc tgatcgccat cgccgccgcc ctggtcgcct tcggggccga tcttatcgag
     1741 accatcacgc tcctggaaat gaccaacgac ctgtccacgg ccgacagcct ggcgctggtc
     1801 gcctcggcgg cggcctggac caaattcacc gccctcacgg tccacgccct ggtcctgtca
     1861 gcggcctgcc tggtggtgac gccgcggcgc tggggtctgt cgctgctgct gctggcccct
     1921 ctggtcggga catcggtggc gatcagcgat gtggcccggc aggctcacat gctgagcctt
     1981 gggctgggcg tcgcctgggg cgcgcttgtg ctgacggccc tgcgcgaggc gcttcggcga
     2041 cggcggccgg cctgatcagc cgccgggcgg acccgggaag ccaggcagac tccagccgaa
     2101 ccgcaacgcc ccgccgcgca c
//
LOCUS       DAORNX010000506         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39282_length_2103_cov_8.291992, whole genome shotgun sequence.
ACCESSION   DAORNX010000506 DAORNX010000000
VERSION     DAORNX010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39282_length_2103_cov_8.291992"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLO65_15870"
     CDS             <1..267
                     /locus_tag="PLO65_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29776.1"
                     /translation="TAGEYFPQPWAGGSRMDDLLGEAAWLIRRDQLVGRLAPFAGDLS
                     RWFSGRDGEAVLVRPDRYVFGVGEPEALAAAWAARTGTTAALAA"
     gene            289..1245
                     /locus_tag="PLO65_15875"
     CDS             289..1245
                     /locus_tag="PLO65_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD29777.1"
                     /translation="MTILRSRALAALALAALSIPATVRGGEPGLTFTTLGTNSGPIAS
                     PTRSQPANLLRYGAQVILVDVGDGAVGQMARAGVAIEPVGAVILSHLHFDHTGGLFAL
                     LGLRRQTMTTTPLTIYGPPGTRRMVDDLMKSLQPGVEVSATIVGKQAPVDSGVTVVEI
                     GGAQTFQLGALTVSAVPNSHFETLAENPQGHLSLSFRFDAPGRSILYTGDTGPSAAVE
                     TLCKGADLLVSEIMDPTESISRLRATRPGVPEGIWAIVDAHFRKEHLSPAEVGELARR
                     CGAGALVVTHNAIADENLDKARAVIAGAYGGPIRFARDLDSF"
     gene            complement(1248..1841)
                     /locus_tag="PLO65_15880"
     CDS             complement(1248..1841)
                     /locus_tag="PLO65_15880"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HRD29778.1"
                     /translation="MSDRLPDASLDQVFRTARTRNGWTPDKVPEALIREIYDLAKMGP
                     TSGNCLPGRFLFLTSDAAKARLAPFMSSTNRAKTLAAPVNVIMAYDMAFADRLPELFP
                     HNPGAKDWFTPNPALTRETAFRNGSLQAAYLMLAARSLGLDCGPMSGVKLDGVNAEFF
                     AGTTWEANFVCNIGYGTDEGLFPRNPRLPFEEACGIL"
     gene            1929..>2103
                     /locus_tag="PLO65_15885"
     CDS             1929..>2103
                     /locus_tag="PLO65_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein B"
                     /protein_id="HRD29779.1"
                     /translation="MATPDDIIMLPARPEPIPVSVSQTAVIVIDMQHAYASPGGYLDL
                     AGFDISGADRVIRN"
BASE COUNT          315 a          734 c          731 g          323 t
ORIGIN      
        1 accgccggcg agtacttccc gcaaccgtgg gccggcgggt cccggatgga tgacctgctg
       61 ggcgaagcgg cctggctgat ccgacgcgat caactcgtcg gccggctggc acccttcgcg
      121 ggcgacctgt cccgctggtt ctcgggtcgc gacggcgagg cggtgctggt gcggccggat
      181 cgctacgtct ttggcgtcgg cgagccggag gcgcttgccg ccgcctgggc cgcgcgcacc
      241 gggacgacgg cggccctcgc ggcctgaacc tgtctggggg aacactgaat gaccatcctt
      301 cgcagccgcg cgctcgccgc gctggccctg gccgccctgt cgatccccgc gaccgtgcgg
      361 gggggcgagc ctggcctcac cttcaccacg ctcgggacca actcggggcc gatcgcctca
      421 ccgacgcggt cgcagccggc caacctgttg cgctacggcg cgcaggtcat cctggtcgac
      481 gtcggggacg gcgccgtggg acagatggcc agggccggcg tggccatcga gccggtcggc
      541 gcggtcatcc tcagccacct gcacttcgac catacgggcg gtctgttcgc cctgctgggc
      601 ctgcgccggc aaaccatgac caccacgccg ctgaccatct acggcccgcc cggcacgcgc
      661 cggatggtcg atgacctcat gaagtcactg cagcccggcg tcgaggtcag cgccaccatc
      721 gtcggcaagc aggcgccggt ggacagcggg gtgaccgtgg tcgagatcgg gggcgcccag
      781 accttccagc tcggcgccct gacggtgtcg gcggttccca acagccattt cgagaccctg
      841 gcggaaaacc cccagggcca cctctcgctg tcgttccgct tcgacgcgcc ggggcgatcg
      901 atcctctata ccggagacac cgggccctcg gcggcggtcg agaccctctg caagggcgcg
      961 gacctgctgg tcagcgagat catggacccg accgagtcga tcagccgcct gcgggcaacg
     1021 cgaccgggtg ttccagaggg aatctgggcg atcgtcgatg cccacttccg gaaggagcac
     1081 ctgtcgccgg ccgaggtcgg cgagctggcg cggcgttgcg gcgccggggc gctcgttgtc
     1141 acccacaacg ccatcgccga cgagaacctc gacaaggccc gcgccgtcat cgcgggcgcc
     1201 tatggcggac cgatccgttt cgcccgtgac ctcgacagct tctgagatca gaggatcccg
     1261 cacgcttcct cgaacggcag ccgcgggttc ctcgggaaca atccttcgtc cgtgccgtag
     1321 ccgatgttgc agacgaaatt ggcctcccat gtcgtgccgg cgaagaactc ggcgttgacg
     1381 ccgtccagct tgacgcccga catcggcccg caatcgagcc ccagggaccg cgcggcgagc
     1441 atgaggtagg cggcctgcag cgaaccgttg cggaaagcgg tctcgcgggt cagggccggg
     1501 ttcggggtga accagtcctt cgcgcccgga ttgtgcggaa acagctcggg caggcggtcg
     1561 gcgaaggcca tgtcgtaggc catgatgaca ttgaccggcg cggccagcgt cttggcccgg
     1621 ttggtcgagg acatgaacgg cgccagtcgc gccttggccg cgtcgctggt caggaacagg
     1681 aaccggcccg gcaggcagtt gcccgaggtc ggccccatct tcgccaggtc gtagatctcg
     1741 cggatcagcg cctcgggcac cttgtccggt gtccagccgt tgcgcgtgcg cgcggtgcgg
     1801 aaaacctggt caaggctggc gtcaggcagg cggtcggaca tggatcgggg actccggctc
     1861 gggacgggtt ggcgccacgt cagcgtatgc catgttacgg ccccgacaac gcaagggccc
     1921 gcttcggcat ggcgacaccc gacgacatca tcatgctgcc cgcccgaccc gagccgatcc
     1981 cggtgtcggt atcgcagacg gcggtgatcg tcatcgacat gcagcacgcc tacgcctcgc
     2041 cgggcggtta tctggatctg gccggattcg acatctccgg cgccgaccgg gtgatccgga
     2101 aca
//
LOCUS       DAORNX010000507         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39312_length_2102_cov_4.425501, whole genome shotgun sequence.
ACCESSION   DAORNX010000507 DAORNX010000000
VERSION     DAORNX010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39312_length_2102_cov_4.425501"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    225..365
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<366..770)
                     /locus_tag="PLO65_15890"
     CDS             complement(<366..770)
                     /locus_tag="PLO65_15890"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918835.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRD29780.1"
                     /translation="MRAALARLAKAVIKVDRQDLRRAALLSLGVTVASLIVPAAALAQ
                     AVNIDLGTGAGLTERVVQLIGLLTVMKGLPLSYSKDMQEDKEAVFDAAETLDLMLAAM
                     TGMISDLTINEAAMKKAAGAGYSTATDLADWLV"
     gene            complement(770..1066)
                     /locus_tag="PLO65_15895"
     CDS             complement(770..1066)
                     /locus_tag="PLO65_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRD29781.1"
                     /translation="MDFLEIVRMVAALAVTLGLIGLASWGMRRFGPDALSRLQVSRRE
                     RRLTLVETLVLDPARRLVLVRLDDEERLILLGEGRTLETLKAPARRRKTPETAA"
     gene            1169..1591
                     /gene="flgB"
                     /locus_tag="PLO65_15900"
     CDS             1169..1591
                     /gene="flgB"
                     /locus_tag="PLO65_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRD29782.1"
                     /translation="MDISGIPLFAMLRQRLGYLGERQRLIAQNVANADTPGYAPRDLK
                     AFTFSAQVQSQAGLAMVQPAQTSAAHLPGPARRIGGSGSFRSALAPDSETTLDGNKVV
                     LEDQMIKMSESRMEYEAAIGFYQKSMALLRMAARPPGR"
     gene            1603..2022
                     /gene="flgC"
                     /locus_tag="PLO65_15905"
     CDS             1603..2022
                     /gene="flgC"
                     /locus_tag="PLO65_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRD29783.1"
                     /translation="MAEIRSQSDAAMAVAASGMRAQQARMRIIAENLANANSTSRTAG
                     GDPYRRQVPVFEPRAVDGGEGVRMSRVEPAAGDFRSTYDPGHPAADPQGYVKLPNVDP
                     LIEALDMKDAQRAYEANLNVIETARSMEMRTLDLLKK"
BASE COUNT          315 a          699 c          656 g          291 t
ORIGIN      
        1 cccgtcccgt cggctcgcgt gcgtgtccgt cgcgaccgct cttagccgag atttctgtcg
       61 tttgcaccct gtcgattgcc gctggtcgat cgagccgtgc cggtcaggcg gttttcaggc
      121 gcttcttcca gtagcggatc tggcggcgca cttcctgcgg agccgtgcca ccgaacgagg
      181 tacggctctt gaccgagttc tgcaccgaaa gaaccgagaa cacgnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnncacca gccagtcggc gaggtcggtg gcggtcgaat agcccgcacc tgcagccttc
      421 ttcatggcgg cttcgttgat cgtcaggtcg gagatcatgc cggtcatcgc ggccagcatc
      481 aggtcgagcg tctcggccgc gtcgaagacg gcttccttgt cctcctgcat gtccttcgaa
      541 taggacagtg gcagcccctt catcaccgtc agcaggccga tcagctgcac cacccgttcg
      601 gtcaggcccg cgcccgtgcc caggtcgatg ttgaccgcct gggccagggc cgcggcgggc
      661 acgatcaggc tggccacggt cacgcccagc gacagcagcg ccgcgcggcg caggtcctgg
      721 cggtcgacct tgatcacggc cttggcgagg cgggcgaggg cggcgcgcat caggcggcgg
      781 tctccggagt cttgcggcgg cgggcggggg ccttgagcgt ctccagcgtg cgaccctcgc
      841 ccagcaggat cagccgctcc tcgtcgtcga gccgcaccag caccagccgg cgggccggat
      901 cgagcaccag ggtctcgacc agggtcagcc gtcgttcccg gcgcgacacc tgcaggcgcg
      961 acagcgcatc ggggccgaac cggcgcatgc cccaggacgc gagcccgatc agccccagcg
     1021 tcaccgcgag ggcggcgacc atgcggacga tttcgaggaa atccatacgt cggaacgccc
     1081 ggactgaggg gaactgaggc agcagccgca tgctctgcgc cggaaccctt aaggcgcgat
     1141 taaccctgtt tgttcaccat gtccgacgat ggatatttcc ggcatcccgc tgttcgccat
     1201 gcttcgccag cgccttggct atctgggcga gcggcaacgg ctgatcgccc agaacgtggc
     1261 caacgccgac acgcccggct acgccccgcg cgatctgaag gccttcacct tctccgccca
     1321 ggtgcagtca caggcgggcc tggcgatggt tcagccggcc cagacctcgg cggcccacct
     1381 gccgggaccg gcgcggcgga tcggcgggtc cggaagcttc cgctcggccc tggcgccgga
     1441 ctcggaaacg accctcgatg gcaacaaggt cgtgctcgag gaccagatga tcaagatgag
     1501 cgagtcgcgg atggagtacg aagccgcgat cggattctac cagaaatcca tggccctgct
     1561 gcggatggcc gcccgtccgc cgggccgttg agcgaggact gaatggccga gatccgatcc
     1621 cagtccgacg cggcgatggc cgtcgccgcc tccggcatgc gcgcgcagca ggcgcgcatg
     1681 cggatcatcg ccgagaacct ggccaacgcc aattcgacgt cccgcacggc cggcggcgat
     1741 ccctaccgcc gccaggtgcc ggtgttcgag ccgcgcgccg tcgatggcgg cgagggggtc
     1801 aggatgagcc gggtcgagcc ggcggccggc gacttccgct cgacctacga ccccggccac
     1861 cccgcggccg acccgcaggg ctacgtcaag ctgcccaacg tcgatccgct gatcgaggcg
     1921 ctcgacatga aggacgccca gcgggcctac gaggccaacc tcaacgtcat cgagacggcg
     1981 cgctccatgg agatgcgcac cctcgacctg ctcaagaagt agagggctga gccatgatga
     2041 ccgccctggc cgccgccaag gcctacgccg ccgccggcaa cggcgcgccg ctcgccggcg
     2101 cg
//
LOCUS       DAORNX010000508         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39422_length_2097_cov_7.751224, whole genome shotgun sequence.
ACCESSION   DAORNX010000508 DAORNX010000000
VERSION     DAORNX010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39422_length_2097_cov_7.751224"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLO65_15910"
     CDS             complement(<1..255)
                     /locus_tag="PLO65_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HRD29784.1"
                     /translation="MSSPKAASESGDPQAIDTRVVRKLADILKDTGLTEIEVESGGLK
                     IRVARELTVQHMAAPVTHPVAATPVAAPVAAPVEAAPTAPA"
     gene            complement(278..733)
                     /locus_tag="PLO65_15915"
     CDS             complement(278..733)
                     /locus_tag="PLO65_15915"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRD29785.1"
                     /translation="MAKPIHVLSGPNLNLLGVREPEIYGRETLADIEARCEARALRLG
                     CSIVFRQSNHEGELIGWVQQARTEAAALVINPAGYGHTSVALLDALKTLDIPIVECHL
                     SNPAARETFRRKTYVSLAATGVVSGFGGSSYELAVEAAAGLIGATGASA"
     gene            799..999
                     /gene="thiS"
                     /locus_tag="PLO65_15920"
     CDS             799..999
                     /gene="thiS"
                     /locus_tag="PLO65_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRD29786.1"
                     /translation="MRVYINGEERVLEPVANVAELVAALGLETRKVAVERNLEIVPRS
                     LHAATPVQDDDRFEIVTFIGGG"
     gene            1228..1440
                     /locus_tag="PLO65_15925"
     CDS             1228..1440
                     /locus_tag="PLO65_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD29787.1"
                     /translation="MATGTVKWFNATKGFGFIAPDAGGGDVFVHISAVERSSLGSIHE
                     GQKLNYELERDQRSGKTSAGQLTSAD"
     gene            complement(1541..1801)
                     /locus_tag="PLO65_15930"
     CDS             complement(1541..1801)
                     /locus_tag="PLO65_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29788.1"
                     /translation="MLKTLRGDGLLAVGDRELAVGYQLDLYADGDRTLGSGSLDADFT
                     GLGDHFEGREGRLTLSDGAAMTVVLEAVEDDGAEFQAQATAG"
BASE COUNT          331 a          704 c          706 g          356 t
ORIGIN      
        1 cgccggcgcc gtcggcgcgg cctcgaccgg cgcggcgacg ggagccgcca ccggcgtggc
       61 ggcgaccggg tgggtcaccg gggcggccat gtgctggacg gtcaactcgc gcgcgacgcg
      121 gattttcagc ccgccgctct cgacctcgat ctcggtcagg ccggtatcct tcaggatgtc
      181 ggccagcttg cgcaccaccc gggtgtcgat ggcctgggga tcgccggact cggaagcggc
      241 cttgggactg gacatgaagc gttaccttgt ctgcggatca ggccgaggcg ccggtggcgc
      301 cgatcaggcc ggcggcggcc tcgaccgcca gttcatagct gctgcctccg aacccggaaa
      361 caacgccggt ggcggccagt gaaacgtagg tcttcctgcg aaacgtctcc cgcgccgccg
      421 ggttcgacag atgacattcg acgatcggta tatcgagagt cttcagcgcg tcgagcaggg
      481 ccacggaagt gtgaccatag ccggccgggt tgatcaccag ggcggcggcc tcggtccggg
      541 cctgctggac ccagccgatc agttcgccct cgtgattgga ttgccggaac acgatcgagc
      601 agccgaggcg cagggcccgg gcttcgcagc gggcctcgat gtcggccagc gtctctcgtc
      661 cgtagatctc cggctcgcgg acccccagga gattgaggtt cggtccgctc agcacatgga
      721 tcggtttcgc catcaggccc cgccgtcgtt tcggcggccc ttgtatcttc gccgggccgg
      781 cgtcacaagc gcagcgtcat gcgcgtgtat atcaacggcg aggaacgggt gctggagccg
      841 gtggccaatg tggccgagct ggtcgcggcg ctgggcctcg aaacccgcaa ggtggcggtc
      901 gagcgcaacc tcgagatcgt gccgcgcagc ctgcatgcgg ccacgcccgt gcaggacgac
      961 gaccgcttcg aaatcgtgac tttcatcggt ggcggctagg ctcgaccgat cagagtttcg
     1021 gcgcggggtt cgagtagagt tgaaaatatt cccttgctgc cctcccgaat tcatgagacg
     1081 tgccacaaat cgaaattcga tctggcgcgg ccagccgttt cggaatctca tcacgggatg
     1141 tccggagtag ccttccaaaa cctctgagaa tttgatcaag cagccgcatc tcgcgggctg
     1201 aaatttatgt ctgcaaggct acaaactatg gctaccggaa ctgtgaagtg gttcaacgcg
     1261 accaagggct tcggcttcat cgcgcctgac gcgggcggcg gcgacgtgtt cgtccacatc
     1321 tcggcggttg agcgttcgtc gctcggctcc atccacgaag gtcagaagct caactacgag
     1381 ctcgagcgcg accagcgcag cggcaagacg tccgctggcc agctgacgtc ggccgactga
     1441 ggctttccgc ttcattcaga cactgacgac gggtcggctt gcgccggccc gttttctttt
     1501 gttcgcctgg cgatccggct ggatcccgcc gggcgccgcg tcagcctgcc gtcgcctggg
     1561 cctggaattc ggcgccgtca tcctcgaccg cctccaggac cacggtcatg gccgcgccgt
     1621 ccgaaagcgt cagtcgtccc tcgcggccct cgaaatggtc gcccagcccg gtgaaatcgg
     1681 cgtccagcga accgctgccg agggtccggt cgccgtcggc gtacaggtcg agctgatagc
     1741 cgacggccag ttcgcgatcg cccacggcca gcaggccgtc gccccgcagg gtcttcagca
     1801 acctgagtga acgcttgccg cgccgtgcgc cgaaaccagg cgcgcccggg cgatcaggtc
     1861 tgtctggcgg cgccacgcgg tcctcccgga cgtcgcggcc cggacacagc gcgacgatgg
     1921 gggaccatgc gccctcaagg ggccgccggc cagttctatc ggtcgaacgg tgttcacacc
     1981 gtgcgccggc gcgcgcgttg aagggcggcg aaggtcgcgg ccgccagcga cccgcagcgc
     2041 caggcgctgt cgcggttctc ggccgatccg ctggcggcga caaccgcgtc cagatcc
//
LOCUS       DAORNX010000509         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39574_length_2090_cov_6.596560, whole genome shotgun sequence.
ACCESSION   DAORNX010000509 DAORNX010000000
VERSION     DAORNX010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39574_length_2090_cov_6.596560"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLO65_15935"
     CDS             <1..719
                     /locus_tag="PLO65_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD29789.1"
                     /translation="LARADGGAVLKALAGLAKTFGVVREGWNGWNVLHTAAARVGGLD
                     LGFLPGEGGLATAGMLKPGALDLLFLLGADECDASASGAFTVYLGSHGDRGAHKADVI
                     LPGAAYTEKNGLYVNTEGRVQMGERAVFPKGEAREDWAILRALSEHVGARLPYDSLDQ
                     LRLRLFADHPTFGQIDFAPGSIPVAVDLSGLGDGGKVSDEPFAGAVTAFHLTNPIARA
                     SVTMAECAALASGAAQMAAE"
     gene            724..1791
                     /gene="nuoH"
                     /locus_tag="PLO65_15940"
     CDS             724..1791
                     /gene="nuoH"
                     /locus_tag="PLO65_15940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRD29790.1"
                     /translation="MPEQTVSPLLWFGLAVGQILLVLIWVLLSLAFLLWGDRKIWAGV
                     QMRKGPNVVGPFGLFQSFADFLKFVLKEVVIPAGADKTVFLLAPLISFVLAFGAWAVI
                     PLAPGWVIANLNVGILYLLAISSLGVYGIIMGGWASNSKYPFLGALRSAAQMVSYEVS
                     IGFVIITVILLAGTMNLSEIVTRQAGWFWNWNLFGGGLQNLPLALVMIPMMVVFYISA
                     LAETNRPPFDLPEAESELVAGYQVEYSATPYLLFMIGEYANIVLMCAMISVLFFGGWN
                     PGFPTPFLDSWPATAQSAFLFLVFFAKIIGWFFMFAMAKAIVPRYRYDQLMRLGWKVF
                     LPMTLVAVMLVAGWRVYGGTL"
     gene            1791..2030
                     /locus_tag="PLO65_15945"
     CDS             1791..2030
                     /locus_tag="PLO65_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29791.1"
                     /translation="MRAAAAISALLLLGACAGVQARMESYSLGRGLVTYDEMRRAKAQ
                     CESEGGSVRPKDAGGDMAQLSNYICEIQPRKAATP"
BASE COUNT          290 a          724 c          703 g          373 t
ORIGIN      
        1 ccctggcccg cgccgacggc ggggcggtgc tcaaggcgct cgcgggtctg gccaagactt
       61 tcggcgtggt gcgggagggc tggaacggct ggaatgtgct gcacacggcc gccgcccgcg
      121 tcggcggcct ggacctcggc ttcctgccgg gcgagggcgg cctggccacg gccggcatgc
      181 tcaagcccgg cgccctggac ctgctgttcc tgctgggcgc cgacgagtgc gacgccagcg
      241 cctcgggcgc cttcaccgtc tacctcggca gccatggcga ccgcggggcc cacaaggccg
      301 acgtgatcct gcccggcgcc gcctacaccg agaagaacgg cctctacgtc aacaccgagg
      361 gccgggtgca gatgggcgaa cgcgcggtgt tccccaaggg cgaggcccgc gaggactggg
      421 ccatcctgcg cgccctgtcc gagcatgtcg gcgcccggct gccgtacgac agcctcgatc
      481 agctgcggct caggctgttc gccgaccatc cgaccttcgg ccagatcgac ttcgcgccgg
      541 gttcgatccc ggtcgccgtc gacctgtcgg gcctcggcga cggcggcaag gtgtcggacg
      601 agccgttcgc gggcgccgtg accgccttcc acctgaccaa cccgatcgcg cgcgccagcg
      661 tgaccatggc cgaatgcgcg gccctggcct ccggcgccgc ccagatggcc gcggagtagg
      721 cgagtgccgg agcagaccgt ctcccccctt ctctggttcg gactggccgt gggtcagatc
      781 ctgcttgtgc tgatctgggt gctgctcagc ctggccttcc tgctctgggg cgaccggaag
      841 atctgggccg gcgtgcagat gcgcaagggt cccaacgtgg tcggcccctt cggcctgttc
      901 cagtcgttcg ccgacttcct gaagttcgtg ctgaaggaag tcgtcattcc ggccggggcc
      961 gacaagacgg tcttcctgct ggccccgctg atcagcttcg tgctggcgtt cggcgcctgg
     1021 gcggtgatcc cgctggcccc cggctgggtg atcgccaacc tcaatgtcgg catcctgtac
     1081 ctgctggcga tcagttcgct cggcgtctac ggcatcatca tgggcggctg ggcgtccaac
     1141 tcgaaatacc cgttcctcgg cgccctgcgc tcggcggccc agatggtctc ctacgaggtc
     1201 tcgatcggct tcgtgatcat caccgtgatc ctgctggccg ggacaatgaa cctcagcgaa
     1261 atcgtcaccc ggcaggccgg ctggttctgg aactggaacc tgttcggcgg cggcctgcag
     1321 aacctgccgc tggcgctggt gatgatcccg atgatggtgg tgttctacat ctcggccctg
     1381 gccgagacga accggccgcc gttcgacctt cccgaagccg agtcggagct cgtggccggc
     1441 taccaggtcg aatacagcgc cacaccctac ctgctgttca tgatcggcga atacgccaac
     1501 atcgtgctta tgtgcgcgat gatcagcgtg ctgttcttcg gcggctggaa ccccggcttc
     1561 ccgacgccgt tcctcgacag ctggcctgcc acggcccaga gcgccttctt gttcctggtg
     1621 ttcttcgcca agatcattgg ctggttcttc atgttcgcca tggccaaggc catcgtgccc
     1681 cgctaccgct atgaccagct catgcggctg ggctggaagg tgttcctgcc catgaccctc
     1741 gtcgcagtga tgctggtcgc cggctggcgc gtctacgggg gaacgctgtg atgcgcgcgg
     1801 cggccgccat ctccgcgctc ctgctgctgg gcgcctgcgc cggggtccag gcgaggatgg
     1861 aaagctacag cctcggccgc ggcctggtga cctacgacga gatgcggcgg gccaaggcgc
     1921 aatgcgagtc cgagggcggc tcggtgcggc ccaaggacgc cggcggggat atggcccagc
     1981 tgtcgaacta tatctgcgag atccagccca ggaaggccgc caccccatga tgcaacgcat
     2041 cggacaggcc atcaaaggcg cggccctgct ggacttcgtg ggcgccttcg
//
LOCUS       DAORNX010000510         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39591_length_2089_cov_6.760079, whole genome shotgun sequence.
ACCESSION   DAORNX010000510 DAORNX010000000
VERSION     DAORNX010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39591_length_2089_cov_6.760079"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLO65_15950"
     CDS             <1..276
                     /locus_tag="PLO65_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HRD29792.1"
                     /translation="NPGPLPPASLVLFGQPGYRGASLVLRGPTRSLAPWSFGDKAKSL
                     QVRGRGRWQVCTLTDYRGRCEIVGDSVYDLGTWRLSNQISSARPIGR"
     assembly_gap    324..336
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(362..1057)
                     /locus_tag="PLO65_15955"
     CDS             complement(362..1057)
                     /locus_tag="PLO65_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD29793.1"
                     /translation="MRVLLIEDDSATAQTIELMLKSEGFNVYTTDLGEEGVDLGKIYD
                     YDLILLDLNLPDMSGMDVLRTLRNGKINTPIMILSGSAEIDTKVKTLGGGADDYMTKP
                     FHKDEMVARIHAVVRRSKGHAQAVIQTGEIAVNLDAKTVEVNGMRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLATASGGQHHIETVWGRG
                     YVLRDPQEGVVAA"
     gene            1530..1883
                     /locus_tag="PLO65_15960"
     CDS             1530..1883
                     /locus_tag="PLO65_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29794.1"
                     /translation="MIDGKTRDAIAAARGRSEFVLSARMIDAAVEMRAGRPDTAAALR
                     EQAWLDVKAAIVEAVGRHGDLRVLRSEKVPPGVILAGPANAWLAFLDAERDLANDRRI
                     ELREYVHTWRFKPLP"
     gene            complement(1888..>2089)
                     /locus_tag="PLO65_15965"
     CDS             complement(1888..>2089)
                     /locus_tag="PLO65_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRD29795.1"
                     /translation="PVLARVRSTREPVAGRLAMIDGEIGVALDVLEEGVAPGQACVLY
                     APEDPSRVLGGGFIATAVRAGG"
BASE COUNT          317 a          720 c          688 g          351 t
ORIGIN      
        1 aatcccgggc cgctgccgcc cgccagcctg gtgctgttcg gccagcccgg ctaccggggc
       61 gccagcctcg tgctgcgcgg gccgacccgc agcctcgcgc cctggtcgtt cggcgacaag
      121 gccaagtcgc tgcaggtccg cggtcgcggc cgttggcagg tctgcaccct gaccgactac
      181 cggggacgct gcgaaatcgt cggcgacagc gtctacgacc tgggaacctg gcggctcagc
      241 aaccagatct cctcggcccg gccgatcggg cgataaccgg tccccgcgcg gaaacgacaa
      301 cgcccgccgg cggaaccggc gggnnnnnnn nnnnnncgta cgcttactgg aaaacgtgaa
      361 gtcaggcggc gacgacgcct tcctgcgggt cgcgcagcac atagccgcgg ccccagacgg
      421 tctcgatgtg atgctggccg ccggaggccg tggccagctt cttgcgcagc ttgcagatga
      481 acacgtcgat gatcttcagc tccggctcgt ccatgccgcc gtagaggtgg ttgaggaaca
      541 tctccttggt cagggtcgtg cccttccgaa gcgagagcag ctccagcatc tggtattcct
      601 tgccggtcag gtggacgcgc ataccgttca cctccaccgt cttggcgtcc aggttcacgg
      661 cgatctcgcc ggtctggatg accgcctggg cgtggccctt ggagcggcgc accacggcgt
      721 ggatgcgggc gaccatctcg tccttgtgga acggcttggt catgtagtcg tcggcgccgc
      781 cgcccagggt cttgaccttg gtgtcgatct cggccgagcc ggaaaggatc atgatcgggg
      841 tgttgatctt gccgttgcgc agggtgcgca gcacgtccat gccgctcatg tccggcaggt
      901 tgaggtccag caggatcagg tcgtagtcgt agatcttgcc cagatcgacg ccttcctcgc
      961 ccaggtccgt cgtatagacg ttgaagccct cggacttcag catcagctcg atggtctgcg
     1021 ccgtcgcgct gtcatcctca atcaacagta cgcgcatcga tcccctcccc gcggcctccg
     1081 cggtaacgtt tcaggcgaga cggaatccgc ctccggagct caattccgac attggaatta
     1141 agctgcttta agactggtta atggtgaacg gcccttcgga aagaatcgtt aagccgattt
     1201 gcgaatcggg ggcaagcggc catctcgcgg ccgtgagtcg cggtcgggat gactctttcg
     1261 tgatcgccga atccccagtg aatcccgtgg gattccgcca gcccttgtga cagtggggga
     1321 atggacccgc cgacccggcc atcgaacccc ggtttcccgg cgccgacgcg ccgcgtgagt
     1381 caatccgacg cgggaagacg atcgccgggc ccgtcggcgc gccgggtccg gagaacgtcc
     1441 gcatggcgcc gtccgagcgt ggttggcgtg cccggttacg cctgtcggcc ccacttgacg
     1501 ccctggcgcg ccccgaccag accgacctca tgatcgacgg caagaccaga gacgccatcg
     1561 ccgccgcccg cggccgcagc gagttcgtcc tctcggcgcg gatgatcgac gccgccgtcg
     1621 agatgcgggc cggccgtccc gatacggctg cggcccttcg tgagcaggcc tggctggacg
     1681 tgaaggcggc catcgtcgag gccgtcggtc gtcacggcga cctgcgggtg ctgcgctcgg
     1741 agaaggtccc tcccggagtc atcctggccg gaccggcgaa cgcctggctc gccttccttg
     1801 acgccgagcg tgacctggcg aacgaccgcc ggatcgagct ccgggaatac gttcacacct
     1861 ggcggttcaa gccgcttccg tagcaggtca gccgcccgcc cgcaccgccg tcgcgatgaa
     1921 gccgccgccg agcacgcggc tggggtcttc cggcgcgtag agcacgcagg cctggccagg
     1981 ggcgacgcct tcctcgagca cgtccagcgc caccccgatt tcgccgtcga tcatcgccag
     2041 ccgcccggcg accggctcgc gggtcgagcg gacgcgggcc agcacgggc
//
LOCUS       DAORNX010000511         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39592_length_2089_cov_5.572271, whole genome shotgun sequence.
ACCESSION   DAORNX010000511 DAORNX010000000
VERSION     DAORNX010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39592_length_2089_cov_5.572271"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLO65_15970"
     CDS             complement(<1..578)
                     /locus_tag="PLO65_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD29796.1"
                     /translation="MIELLEHPLSPYAQKVKIALAEKGVVFTVRTPQAIGSGATPADF
                     RAASPRGEVPVLFADGQPIFDSTIILEYIEDRFPTPALLPADPLGRARARMIEEVCDT
                     HFEAINWGLSEITYSRRAEGALETTLRAAAAAQIGRLHLWLEGQLGDADWFGGETFGW
                     ADIGVVPYVQGAAGFGLAPPAGGRLDAWLARAR"
     gene            943..>2089
                     /locus_tag="PLO65_15975"
     CDS             943..>2089
                     /locus_tag="PLO65_15975"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRD29797.1"
                     /translation="MTPPASPQRRADLDWIRVAAFGLLILFHVSLVYAPWDWHVHSKH
                     TFAWLEEATAATGPWRLTLLFLVSGAALRFMSRRYSPVEVLRARMIRLLPPLLFGVLV
                     LVPPQSWLEATDKGSFGGGLAAWWLREFSPSGLADGVPVNHLWFVEYIVVYSLAAVAL
                     MTRPAWIAALERGVERWLSGWRLLVLPILFFVLARQLVFPPFGQTNQLFGDWYNHLTS
                     LGAFLFGFVVAGREAVWKTFEAQRWMALALALVSTPLLMALTAHPGGLAFDGWVKHGM
                     HGVEQWSAIAAILGFGSRHLRGADGPLLRYLTDAVFPCYLAHQTLLVIAVRLVKPIDL
                     PAPVEAAILVSATLGGSLLVYELARRSGPLRPLWGLRRHPAVRAAPPP"
BASE COUNT          252 a          756 c          738 g          343 t
ORIGIN      
        1 cgggcgcggg ccagccaggc gtccagacgg cccccggccg ggggcgccag cccgaatccg
       61 gccgcgccct ggacgtaggg gaccacgccg atatccgccc agccgaacgt ttcgcccccg
      121 aaccagtcgg cgtcgcccag ctggccctcc agccagaggt gaaggcgtcc gatctgggcg
      181 gcggcggcgg cgcgaagggt cgtttccagc gccccctccg cgcggcggga ataggtgatc
      241 tccgacaggc cccagttgat cgcctcgaaa tgggtgtcgc agacctcctc gatcatccgc
      301 gcccgggccc tgccgagcgg atcggccggc agcagcgcgg gggtgggaaa gcggtcctcg
      361 atgtattcca ggatgatggt cgaatcgaag atcggttggc cgtcggcgaa cagcaccggc
      421 acctcgccgc gggggctggc ggcgcggaag tccgccggcg tggcgcccga gccgatcgcc
      481 tgcggcgttc ggacggtgaa gacaacgccc ttctcggcca gggcgatctt gaccttctgg
      541 gcgtagggcg acagcgggtg ttccaggagt tcgatcatcg ggccagtggg cgggatcgcg
      601 ccgcatccgt caacggcgcc cctgcggaag tgggcgcgaa accggccccg gtgcgatcgc
      661 tcgcaccggg gccgctcacc gcggttggcc aggacagccc acccgctcgc cccggacccc
      721 ctccgacagg acaccgggac gcgctatcat gcgccggacc gggcccgggt cagcccccgg
      781 cccgcgtcga cgacgccttt ctcgcgaagt cagttcgcag gtgcacaatt tcggggcgct
      841 tccgctccgt ccccgcacag ccgttgagcg gaaagctcaa cgaatcccgg cggcccggcc
      901 cggttgcgcg gagcggctga ttttgcgatg cagcgttccg ccatgacccc gcccgcctcc
      961 ccacagcgcc gcgccgacct cgactggatc cgcgtggcgg ctttcgggct gctgatcctg
     1021 ttccacgtca gcctggtcta cgcgccctgg gactggcacg tgcacagcaa gcacaccttc
     1081 gcctggctgg aggaagccac ggcggcgacc ggcccctggc ggctgaccct gctgtttctg
     1141 gtgtcggggg cggcgctgcg gttcatgagc cggcggtatt ctccggtcga ggtcctgcgc
     1201 gcgcggatga tccgactgtt gccgcccctg ttgttcgggg tgctcgtgct ggtgccgccg
     1261 cagtcctggc tcgaggcgac cgacaagggc agcttcggcg gcggcctcgc ggcctggtgg
     1321 ctgcgcgagt tcagcccgtc cggcctggcc gacggcgtgc cggtcaacca cctgtggttc
     1381 gtcgagtaca tcgtcgtcta cagcctggcc gccgtggcgc tgatgacccg gccggcctgg
     1441 atcgcggcgc tcgagcgcgg cgtcgagcgc tggctgtcgg gctggcggct gctggtcctg
     1501 ccgatcctgt tcttcgtcct cgcgcggcag ctggtgtttc caccgttcgg ccagaccaac
     1561 cagctgttcg gcgactggta caaccatctg acgtcgttgg gggccttcct gttcggcttc
     1621 gtcgtcgccg gccgcgaggc ggtgtggaag accttcgagg cccagcgctg gatggcgctg
     1681 gcgctcgccc tggtgtcgac gcccctgctg atggcgctga ccgcccatcc cggcggcctg
     1741 gccttcgacg gctgggtcaa gcacggcatg cacggcgtgg agcagtggtc ggcgatcgcc
     1801 gcgatactcg gcttcggcag tcgtcacctg cgcggcgccg acgggccgct gctgcgctat
     1861 ctgactgacg cggtgtttcc ctgctatctc gcccaccaga ccctgctggt catcgccgtg
     1921 cgcctggtca agccaatcga cctgcccgcc cccgtcgagg ccgcgatcct ggtgtcggcc
     1981 accctcggcg gcagcctgct ggtctatgaa ctggcccggc gcagcggtcc gctgcggccg
     2041 ctgtggggcc tgcgtcgtca tccggcggtt cgcgccgcgc cgccgccgg
//
LOCUS       DAORNX010000512         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39714_length_2083_cov_5.425049, whole genome shotgun sequence.
ACCESSION   DAORNX010000512 DAORNX010000000
VERSION     DAORNX010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39714_length_2083_cov_5.425049"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PLO65_15980"
     CDS             complement(<1..93)
                     /locus_tag="PLO65_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD29798.1"
                     /translation="MTHRTVQIVGAGPAGLMAAERLAAAGLAVVV"
     gene            222..1520
                     /locus_tag="PLO65_15985"
     CDS             222..1520
                     /locus_tag="PLO65_15985"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRD29799.1"
                     /translation="MTVRIDRRRMMLAGGGAVALGAAATLALRPGDHGGVHSPYFLGL
                     QSALGTAGLARPTLVIDRRRLDANITAVKTFLPARQGLRVVVKSLPSLGLLDAVARGV
                     GTSRFMVFNGPMLATMAAARPDGDYLMGKPLAAPEAKAFYDAGPGKAAGLDPARQLQW
                     LIDTPARLDQYAAVARAAGTTMRVSLEIDVGLHRGGFNDPAGFAVALHRIAGDPVLSL
                     GGLMGYDPHLSHVKAGGGRDKALARSRVLYQGFLDQVREAQGAGFNPAALTLNTAGSP
                     TYRLHHDSPYANDISVGSAFVKPLDFDVDTLAAHVPAAYIATPVLKALPRTDLPTAES
                     LTPLFRLWDRNTARAFFIHGGHWLAAPVSPPGLQYSSLFGRSSNQELLTGSEKVSLSP
                     DDTVFLRPTQSEAVLLQFGDLAVWDGKAISAMWPVFPVSA"
     gene            complement(1483..>2083)
                     /locus_tag="PLO65_15990"
     CDS             complement(1483..>2083)
                     /locus_tag="PLO65_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29800.1"
                     /translation="CVDADDRHYVTIDIEGAPIRFVVSPAEERMAAGNVDFLYEATVL
                     AADQAAAVDQIARTFNLDPSAFTTITSETFGYTGVLTLFHPERLHRFEVITPIDMDKT
                     MGRYYRREGASYYMAFAESAEMPLIERLAAEAGAGITLDRPDDRDPALTADQLWLHPA
                     TLGGMMLGVSRPSMAWRWSGKPERVEPVRPTPETPATSR"
BASE COUNT          289 a          755 c          734 g          305 t
ORIGIN      
        1 gaccacgacc gccaggccgg cggccgccag ccgttcggcg gccatcaggc cggcggggcc
       61 agcccccacg atttgcacgg tgcgatgcgt catggagctt tcctagaccg gcgcggggcc
      121 ggcgggaacg gtcgcggacc gactgcaccg tcggccctcc ccagccgccc gcgaacaggg
      181 caggctgtcg cctgacaggg actcacccgc cggagaccgc catgaccgtc aggatcgatc
      241 gccgccgcat gatgctggcc ggaggcggcg ccgtggccct tggcgcggcc gcgacgctgg
      301 ccctgaggcc cggcgaccat ggcggcgtcc attcgcccta cttcctgggg ctgcagtcgg
      361 cgctcgggac ggcgggactc gcgcggccga cgctggtcat cgaccggcga cggctcgacg
      421 ccaacatcac ggcggtgaag accttcctgc cggcccgcca gggattgcgg gtggtggtca
      481 agtcgctacc gtcgctcggc ctgctcgacg ccgtggcgcg gggcgtcggc acgagccggt
      541 tcatggtctt caacggcccg atgctggcga ccatggcggc ggcccggccc gacggcgact
      601 acctgatggg caaaccgctc gcggcgccgg aggcgaaggc gttctacgac gcggggccag
      661 gcaaggcggc cggacttgat ccggcgcgcc agctgcaatg gctgatcgac accccggccc
      721 gtctggacca gtacgcggcc gtcgcccgcg ccgccgggac gacgatgcgc gtcagccttg
      781 agatcgacgt cggcctgcat cgcggtggct tcaatgaccc ggcaggcttc gccgtcgccc
      841 tgcaccgcat cgccggcgac cctgtcctca gtctcggcgg gctgatgggt tacgatccgc
      901 acctgagcca cgtgaaggcc ggtggcgggc gggacaaggc cctggcccgc agccgggtgc
      961 tctaccaggg cttcctcgac caggttcgcg aggcccaggg cgcggggttc aacccggcgg
     1021 ccctgaccct caacaccgcc ggctccccga cctaccggct gcaccacgac agcccctacg
     1081 ccaacgacat ctcggtcggc tcggccttcg tcaagccgct ggacttcgat gtcgacaccc
     1141 tggccgccca tgtcccggcc gcctatatcg ccacgccggt gctcaaggcc ctgccgcgca
     1201 ccgacctgcc gacagccgag tcgctgacgc cgctgttccg cctgtgggac cgcaacaccg
     1261 cgcgggcctt cttcatccac ggcggccact ggctggccgc gccggtctcg ccgccgggcc
     1321 tgcagtattc gtcgctgttc ggccggtcct cgaaccagga gctgctgacc ggatcggaaa
     1381 aagtctcgct gtcgcccgac gacacggtgt tcctgcggcc gacccagagc gaggcggtgc
     1441 tgctgcagtt cggcgacctg gccgtctggg acggcaaggc gatcagcgcg atgtggccgg
     1501 tgtttccggt gtcggcctga ccggctcgac ccgctcgggc ttgcccgacc agcgccaggc
     1561 catcgaaggc cgcgaaacgc ccagcatcat gccgcccagg gtcgccgggt gcagccagag
     1621 ctggtcggcg gtcagggccg ggtcgcggtc gtccggccgg tccagcgtga tgccggcccc
     1681 ggcctcggcc gccagccgct cgatcagcgg catttccgcg ctctcggcaa aggccatgta
     1741 gtaggacgcg ccctcccgcc ggtagtagcg gcccatggtc ttgtccatgt cgatcggcgt
     1801 gatcacctcg aagcggtgca gccgctcggg atggaacagg gtcagcactc cggtgtagcc
     1861 gaacgtctcc gaggtgatgg tggtgaaggc cgacggatcg aggttgaagg tgcgggcgat
     1921 ctggtcgacc gccgcggcct ggtcggcggc cagcacggtg gcctcgtaga ggaagtcgac
     1981 attgccggcg gccatccgct cttccgccgg cgagacgacg aagcggatcg gggcgccctc
     2041 gatgtcgatc gtcacatagt ggcggtcgtc ggcgtcgacg cag
//
LOCUS       DAORNX010000513         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_39989_length_2072_cov_3.825483, whole genome shotgun sequence.
ACCESSION   DAORNX010000513 DAORNX010000000
VERSION     DAORNX010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39989_length_2072_cov_3.825483"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLO65_15995"
     CDS             <1..683
                     /locus_tag="PLO65_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRD29801.1"
                     /translation="AGAPAKTREAVVRPVAAGVASGLLYRQWVNDRRAYVERVSGGRL
                     GYVHIADMGDASLAQLYIDLDAQNQGREGVVIDLRNNNGGYVNGHALDVFTRRNYLLM
                     TARDRFPVPSRQNLGQRALGLPTVLVINESSLSDAEDFTEGYRSLGLGKVVGKPTAGW
                     IIYTGGRELIDGSVVRLPFIRVEDLRGQTMEGNPRPVDLDVDRPLGETLTGQDAQLDA
                     AVKVLLGG"
     gene            820..>1518
                     /locus_tag="PLO65_16000"
     CDS             820..>1518
                     /locus_tag="PLO65_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29802.1"
                     /translation="MRKTSTAIAAALLVLVTPVMAQQADTPPGGWSAVFDDSALREAS
                     PESIDASIAWIDYLLRRTREGDILMENRDLDIAGGAEIVFPVNRDILIRAMQLEVSDG
                     NISARDGARKLILIARASGRLRAGLQEQRQRLVVRRRELRTGGSLPPPPPPPPLQPWE
                     LGRPPATREVACVAEGVWGNTIRAGSSTWTIAANGSAAESGMGNARGQATLANDVLTI
                     TFRTGAVAGVYRIDV"
     assembly_gap    1519..1589
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1781..>2072
                     /locus_tag="PLO65_16005"
     CDS             1781..>2072
                     /locus_tag="PLO65_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD29803.1"
                     /translation="MARSPEIQAGVADVSAAFVHDLANAGALPMWAPHRPQRPAKSEG
                     GRRFNLVSDYSPAGDQPTAIRELVEGIQAREHDQVLLGVTGSGKTFTMASIIA"
BASE COUNT          302 a          749 c          677 g          273 t
ORIGIN      
        1 cggcgggcgc gcccgcgaaa acccgcgagg ccgtcgtgcg gccggtcgcc gccggcgtcg
       61 ccagcggcct gctctatcgc cagtgggtca acgaccgccg cgcctatgtc gagcgggtca
      121 gcggcggacg gctcggctat gtccatatcg ccgacatggg cgacgcctcg ctggcccagc
      181 tgtacatcga cctcgacgcc cagaaccagg gccgcgaggg cgtggtcatc gacctgcgca
      241 acaacaacgg cggctacgtc aatggccatg cgctcgacgt gttcacccgg cgcaactacc
      301 tgctgatgac cgcccgcgac cgcttcccgg tgccgagccg gcagaacctc ggccagcggg
      361 cgctcggcct gccgaccgtg ctggtgatca acgagtcgtc gctgtccgac gccgaggact
      421 tcaccgaggg ctatcgcagc ctcggcctgg gcaaggtggt cggcaagccg acggccggct
      481 ggatcatcta caccggcggg cgggagctga tcgacggctc ggtggtgcgg ctgccgttca
      541 tccgcgtcga ggacctgcgc ggccagacca tggagggcaa cccccgcccg gtcgacctcg
      601 acgtcgaccg cccgctgggc gaaaccctga ccggccagga cgcccagctg gacgcggcgg
      661 ttaaggtgct gctgggcggg tgacgacccg gctgattggc caaacccgcc attcgtgaga
      721 cagtgcgcgg cgtagagtca tgatcagcgt gcggatcggg cgggcctcgc ctgggtgctg
      781 cgtcgtgaac ggcccgttcc tccgcgaggg ggagacatca tgcgaaagac ctcgaccgcc
      841 atcgccgcgg ccctgctggt ccttgtgacg cccgtcatgg cccagcaagc cgacacgcca
      901 cccggcggct ggtcggccgt cttcgacgac tccgcgcttc gcgaggcgag ccccgagagc
      961 atcgacgcgt cgatcgcctg gatcgactac ctgcttcgcc ggacccggga gggcgatatc
     1021 ctgatggaga accgcgacct cgacatcgca ggtggggccg agatcgtctt tccggtcaat
     1081 cgcgacatcc tgatccgggc catgcagctt gaagtcagcg acggcaatat cagcgccagg
     1141 gacggcgccc ggaagctgat cctgatcgcc cgggcgagcg gccggctgag ggctggcctg
     1201 caggagcagc gccagcgcct ggtggtgcgc cgacgggagc tgcgcaccgg cggctcgctg
     1261 cccccgcccc cgcccccgcc tccgctgcag ccctgggaac tcggccggcc gccggcgacc
     1321 agggaggtcg cctgcgtcgc cgagggcgtc tggggcaaca ccatccgcgc cggcagttcg
     1381 acctggacca tcgccgccaa cgggtccgcc gcggaatcag gcatgggcaa cgcgcgcgga
     1441 caggccaccc tggccaatga cgtgctcacc atcaccttcc gcacaggcgc tgtggccggg
     1501 gtctacagga tcgacgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atctcccgcg gcgccagcct ttgctggtgg
     1621 taatctccgg cccgtccggc gtcaagtacc aggccatgcc agggacgggc ggcgcgccga
     1681 agaattgagc cctgccgcca ccccgccgcc accctcctgc caccccgctg tcagcaggcg
     1741 cccctagcca cgccacaccg gcgcgcctac cttcctgttc atggcccgtt cccccgagat
     1801 ccaggcaggc gtcgcggacg tctccgccgc cttcgtccat gacctggcca acgccggggc
     1861 cctgccgatg tgggccccgc accggccgca gcggccggcc aagtccgagg gcggccggcg
     1921 gttcaacctg gtcagcgact acagccccgc cggcgaccag ccgacggcca tccgcgagct
     1981 ggtcgagggc atccaggccc gcgagcacga ccaggtgctg ctcggcgtca ccggctcggg
     2041 caagaccttc accatggcca gcatcatcgc cg
//
LOCUS       DAORNX010000514         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_40053_length_2069_cov_6.645482, whole genome shotgun sequence.
ACCESSION   DAORNX010000514 DAORNX010000000
VERSION     DAORNX010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40053_length_2069_cov_6.645482"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1459
                     /locus_tag="PLO65_16010"
     CDS             245..1459
                     /locus_tag="PLO65_16010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD29804.1"
                     /translation="MAGAKMRFGAFIAPFHPLRENPTLALERDLELVQHMDRLGFEEA
                     WIGEHHSAGYELIASPELFIATVAERTKHIRLGTGVASLPYHHPLMLADRINQLDHIT
                     RGRVMFGVGPGALPSDAFMMGIPVAAQRDRMDEAIDVLVPLLRGEQVTKKTDWFELNE
                     ARLQMTPFSRPSVEICAASQVSPTGATAAGRHGLGLLSIGATQSGGFNALASNWAIAE
                     EKARESGHTMDRSGWRLVGPMHIAETREQARADVRFGLDDWLYYFREVAALPLAPQDG
                     TDPVDALIASGMAVIGTPEDAIAQIERLEQQSGGFGAFLFMDHNWANWDAKRKSYEMF
                     ARYVAPKFQNLNSNREASLTWAAENRPRFMGEAMAAVGMRVAQHIQEKGTENIRPEIL
                     EAMGMNKKPAAE"
     gene            complement(1513..1740)
                     /locus_tag="PLO65_16015"
     CDS             complement(1513..1740)
                     /locus_tag="PLO65_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29805.1"
                     /translation="MFETLPGGETFRREVATEVEYLTKMHGADALKVAREKASRSNLR
                     TSRRKVLEATVRCLEQGLKSPRVSVWSRLFG"
BASE COUNT          334 a          739 c          678 g          318 t
ORIGIN      
        1 tcgaagaact ggcgcggcag gtccagacca tccgctgctc ggcctgcggc gcgcgggaag
       61 ccggcatcga ggtatggagc ggccggcaac cgccgtcggc cggccgcggg ccgttcccct
      121 ttcgctgaac gcggaaaatc gtccggctgc cgctgcggcg ccccgcattt tcatctggca
      181 agcgcccgcc ccgtcgttag actgaacgca gataaccaac attttccccg gggagtgata
      241 catcatggcc ggcgccaaaa tgcgtttcgg ggcgttcatc gcccccttcc accccctccg
      301 ggaaaatccc accctggcgc tggaacggga ccttgaactg gtccagcaca tggaccggct
      361 gggcttcgag gaggcctgga tcggcgagca ccattcggcc ggctatgaac tgatcgccag
      421 ccccgaactg ttcatcgcca cggtcgccga gcgcacgaag cacatccgcc tcggcaccgg
      481 cgtggcctcc cttccctacc accatccgct gatgctggcc gaccggatca accagctcga
      541 tcatatcacc cgcggccggg tgatgttcgg cgtcggcccg ggcgccctgc cttcggacgc
      601 cttcatgatg ggcatccccg tggccgccca gcgcgatcgg atggacgagg cgatcgacgt
      661 cctcgtgccg ctgctgcgcg gcgagcaggt gacgaagaag accgactggt tcgagctcaa
      721 cgaggcgcgt ctgcagatga cgccgttcag ccgtccgtcg gtcgagatct gcgccgccag
      781 ccaggtctca ccgaccggcg cgaccgccgc cggccgccac ggcctgggcc tgctgtcgat
      841 cggcgcgacc cagtcgggcg gcttcaacgc cctggcctcc aactgggcga tcgccgagga
      901 aaaggcccgc gagagcggcc acacgatgga ccgctcaggc tggcgccttg tcgggcccat
      961 gcacatcgcc gagacgcgcg agcaggccag ggccgacgtc cgcttcggcc tcgacgactg
     1021 gctgtactat ttccgcgagg tcgcggccct gccgctggcg ccccaggacg gcaccgaccc
     1081 ggtcgacgcc ctgatcgcct cgggtatggc cgtgatcggc acgcccgagg acgccatcgc
     1141 ccagatcgag cggctcgagc agcagtccgg cggcttcggc gccttcctgt tcatggacca
     1201 caactgggcc aactgggacg ccaagcggaa gtcctacgag atgttcgccc gctacgtcgc
     1261 gccgaagttc cagaacctca acagcaatcg cgaagcctcg ctgacctggg cggccgagaa
     1321 ccgcccgcgc ttcatgggcg aggcgatggc cgcggtcggc atgcgcgtgg cccagcacat
     1381 ccaggaaaag ggcacggaga acatccgccc cgaaatcctt gaggccatgg gcatgaacaa
     1441 gaagccggcc gcggaatagg ttggggcgcg ggcgacgggg ccgagccggg accctgtccc
     1501 ggccgtccgg agctagccga acagccggct ccagacgctg acccgcgggg atttcagtcc
     1561 ctgttcaagg caccgtacgg tcgcctcgag cacctttcgc cgcgaggtgc gcaggttgga
     1621 tcgcgacgcc ttttcgcggg ccaccttcag agcgtcggcc ccgtgcattt tcgtcagata
     1681 ttcgacttcg gtggcgactt cacgccggaa cgtctcgccg ccggggaggg tttcgaacat
     1741 gctcgtcctc tggtttggcc cgaagtccag tttcccaacg cggcatcgcc catagcgctc
     1801 gcttgcgccg gttcaaagaa gcgtgaggcc gaaatcgggt atggacgtgt aaacgcgcgc
     1861 aattgttcgg cccgtggcca gacggcgcct gctttgtgac gcgtgggcgc tcacgccgcg
     1921 gggcgcgggg aaccccgccg gcgagcgcct gcggcccccg cgtgtcgcgg atgcgccgga
     1981 acgtccccgc cgcgagcgcc atcgcctcct cccacgtggc ctcgcggaac accgactgcc
     2041 actcgccgcg ggcgagctgc atgttggca
//
LOCUS       DAORNX010000515         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_40314_length_2058_cov_5.151772, whole genome shotgun sequence.
ACCESSION   DAORNX010000515 DAORNX010000000
VERSION     DAORNX010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40314_length_2058_cov_5.151772"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLO65_16020"
     CDS             complement(<1..317)
                     /locus_tag="PLO65_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29806.1"
                     /translation="MADIRFDGKVAIVTGAGGGLGRQHALELARRGAKVVVNDLGGSM
                     DGTGGNSAAAEAVVAEIKAFGGEAMANGSSVTDDAGVAKMVKDTMDAWGRIDILIANA
                     GILR"
     gene            complement(399..674)
                     /locus_tag="PLO65_16025"
     CDS             complement(399..674)
                     /locus_tag="PLO65_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRD29807.1"
                     /translation="MHDQTKTSVLKRLNRIEGQVRGLSKMIADDRYCIDVITQIAAVQ
                     AALRKVEEEVLKDHVAHCVEHAIASGDADEQRRKVAELMEVFGRSRG"
     gene            766..>2058
                     /locus_tag="PLO65_16030"
     CDS             766..>2058
                     /locus_tag="PLO65_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRD29808.1"
                     /translation="MTTHEHSCHHDHAAAHAVEEKDPVCGMSVDPATAKHHALHDGHD
                     YWFCSPGCLTKFVADPDRYLTPAAAEPAHAGAIWTCPMHPEIRQKGPGSCPICGMALE
                     PETVGRDDGPNPELADMTRRLWIAAALTLPVFALDMGQHLLGLTPPWSGRTTAWIELL
                     LASPVVLWSGAPFFRRGWASLASRNLNMFTLIALGTGAAWLYSLVATVLPGVLPPAFR
                     GHDGTAPIYFEAAAVITVLVLLGQVLELRAREQTGGAIRALLDLAPRTARRIAADGSE
                     AEVGVDEIHPGDRLRVRPGEKIPVDGEVLEGRSHVDESMVTGESAVDQSRITGESMPV
                     LKVAGAAVVGGAINQSGGFVMRADRVGRDTLLARIVAMVAQAQRSRAPIQRLADRVAG
                     WFVPLVIAVAVAAFVGWAIWGPEPRMAHALVAAVSVLII"
BASE COUNT          278 a          731 c          707 g          342 t
ORIGIN      
        1 cgcaggatgc cggcgttggc gatcaggatg tcgatgcggc cccaggcgtc catcgtgtcc
       61 ttgaccatct tggccacgcc ggcgtcgtcg gtcaccgagc tgccgttggc catggcctcg
      121 ccgccgaagg ccttgatctc ggccaccacg gcctcggccg cggccgagtt gccgcccgtg
      181 ccgtccatcg agccgcccag gtcgttgacc acgaccttcg cgccgcgccg cgccagttcc
      241 agggcgtgtt gccgccccag gccgccgccg gcccccgtga cgatcgcgac cttaccgtcg
      301 aagcgaatgt cggccatgtc tctgttcctc tgcgctgaac gcctgtctgg atttcggcgg
      361 cgttttgcgg cgcagcaggg gaggcgtcaa gggcgccctt acccccgact ccgcccgaac
      421 acctccatca gctcggcgac cttgcggcgc tgctcgtcgg cgtcgccgct ggcgatggcg
      481 tgttcgacgc agtgggccac gtggtccttc aggacttcct cctcgacctt gcgcagcgcg
      541 gcctggacgg cggcgatctg ggtgatcacg tcaatgcagt agcgatcgtc ggcgatcatt
      601 ttcgacagcc cccgcacctg cccctcgatg cggttcaggc gtttgaggac cgaagtcttg
      661 gtctggtcat gcatgctcat acctccaggg ggtagttgat agataccggt ggggggtata
      721 tatccggcgg cgaccgatcg cgaaagaccc tgcgaggatt ccggcatgac cacccacgaa
      781 cacagctgcc accacgacca cgctgccgcc cacgccgttg aggagaagga ccccgtctgc
      841 ggcatgtccg tcgacccggc gaccgcgaaa caccacgccc tgcatgatgg gcatgactac
      901 tggttctgct ctcccggctg tctgacgaaa ttcgttgccg acccggaccg ctacctgacg
      961 ccggcggcgg ccgaacccgc gcacgcgggg gcgatctgga cctgcccgat gcatccggag
     1021 atccgccaga aaggaccagg gtcctgcccg atctgcggca tggcgctgga gcccgagacg
     1081 gtcggccgcg acgacgggcc caatcccgag ctggccgaca tgacccggcg cctgtggatc
     1141 gccgccgccc tgaccctgcc ggtgttcgcg ctcgacatgg gccagcatct gctggggctc
     1201 acgccgccgt ggagcggacg gacgacggcc tggatcgaac tgctgctggc cagccccgtg
     1261 gtgctgtggt ccggggcgcc gttcttccgg cgtggctggg cctcgctggc cagccgcaac
     1321 ctcaacatgt tcaccttgat cgccctgggg accggggcgg cctggctcta ctcgctggtg
     1381 gccacggtcc tgcccggcgt gctgccacca gccttccgcg gccatgacgg gaccgcgccg
     1441 atctatttcg aggcggcggc cgtgatcacc gtgctcgtgc tgctgggcca ggtgctggag
     1501 ctgcgcgcgc gcgaacagac cggcggggcc attcgcgccc tgctcgatct cgcgccacgg
     1561 acggcccggc ggatcgccgc cgacggctcg gaggccgagg tcggggtcga cgagatccac
     1621 cccggcgacc ggctgcgcgt gcggcccggc gagaagatcc ccgtcgacgg cgaggtgctc
     1681 gaaggccgca gtcacgtcga tgagtcgatg gtcacaggcg aatcggcggt ggaccagtcg
     1741 cgcatcaccg gcgagtcgat gcccgtgctg aaggtggccg gcgcggctgt tgtcggcggg
     1801 gcgatcaacc agtccggcgg ctttgtcatg cgcgcagacc gcgtcgggcg tgacaccctg
     1861 ctggcgcgga tcgtcgcgat ggtcgcccag gcccaacgaa gtcgcgcgcc gatccagcgg
     1921 ctggccgacc gcgtcgccgg ctggttcgtg ccgctggtca tcgccgtcgc cgtggccgcc
     1981 ttcgtcggct gggcgatctg ggggccggag ccgcgcatgg cacacgccct tgtggcggcc
     2041 gtcagtgtgc tgatcatc
//
LOCUS       DAORNX010000516         2053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_40435_length_2053_cov_6.293293, whole genome shotgun sequence.
ACCESSION   DAORNX010000516 DAORNX010000000
VERSION     DAORNX010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40435_length_2053_cov_6.293293"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>378
                     /locus_tag="PLO65_16035"
     CDS             <1..>378
                     /locus_tag="PLO65_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRD29809.1"
                     /translation="GPRLTGAPRRVEVKRHDAEAAAWLEVSHDGWVKRFGLVHERKLF
                     LDRGQEELRGEDSFRPEVEVRTDRPRRYMPFTVRFHLHPDARASLARDRKSVLLKGPS
                     EQGWWLRNDAIEVSVEPSLHIEAG"
     assembly_gap    379..394
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      596..675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            694..>2053
                     /gene="purH"
                     /locus_tag="PLO65_16040"
     CDS             694..>2053
                     /gene="purH"
                     /locus_tag="PLO65_16040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRD29810.1"
                     /translation="MPAAPDYPASPDETPVRRALLSVSDKTGLIDAARALVELGVELV
                     STGGTRAAIAAAGLPVRDVSDLTGFPEMMDGRVKTLHPVVHGGLLGVRDAADHAKAMA
                     DHGIGGIDLLYVNLYPFEATVAAGGDFATCVENIDIGGPAMIRSAAKNHGYVAVCTDP
                     ADMAEVLLQLKDGGTTTLDLRKRLAARAYARTAAYDAAIGAWFADQLGDTAPARKAIA
                     GTLIQTMRYGENPHQSAAFYAGGPARPGVANARQLQGKELSYNNIADTDAAFELVAEF
                     SEPACVIVKHANPCGVATGGDLLGAYQRALECDPVSAFGGIVAVNRRLDRKAAEAIVE
                     VFTEVVIAPEADDDAVAVFEAKKNLRLLVTGALPDPRAPGEVFRSVAGGFLVQSRDAS
                     LILPGDLKIVTKRAPSPAEITDMLFAFTIAKHVKSNAVVFARGGQTLGVGAGQMNRKD
                     SAR"
BASE COUNT          298 a          765 c          708 g          266 t
ORIGIN      
        1 ggcccgcggc tgaccggcgc gccccgcagg gtcgaggtca agcgccacga cgccgaggcg
       61 gccgcctggc tggaggtgtc gcacgacggc tgggtcaagc gcttcggcct ggtccacgag
      121 cgcaagctgt tcctcgaccg ggggcaggag gagctgcgcg gcgaggacag cttccggccc
      181 gaggtcgagg tgcggaccga ccggcccagg cgctacatgc cgttcaccgt gcgcttccac
      241 ctgcatcccg acgcccgcgc ctcgctggcg cgcgaccgca agagcgtgct gctcaagggg
      301 ccgtcggagc agggctggtg gctgcgcaac gacgccatcg aggtgtcggt cgaaccctcg
      361 ctgcacatcg aggcgggcnn nnnnnnnnnn nnnnggtgca ggtcgtcctg cgcggccagg
      421 ccagcgacga caagggcggc cgggtgcgct ggaagctgtc gcgggccgag gggtagggct
      481 tagcagtggg caggcgcggt cgcggcggcg tgaaccggtc gaactactcc ctacgcccca
      541 cgcccgagac ccgcgcggtc gcgcattgac tcccccgccc ctccccctat tttccggccg
      601 ttcgcgtgac tggcgctgaa ggtggttgac caccggggag cgcggacctc atcgccgcgc
      661 gcctgggctc cccttcctca cccggagacc cgcatgcccg ccgcccccga ctatcccgcc
      721 agccccgacg aaacccccgt ccgccgcgcc ctgctgtccg tctcggacaa gaccgggctg
      781 atcgacgccg cccgcgcgct tgtcgagctg ggcgtcgagc tggtctcgac cggcggcacc
      841 cgggcggcga tcgcggcggc gggcctgccg gtgagggacg tctcggacct gaccggcttt
      901 cccgagatga tggacggacg ggtcaagacc ctgcaccccg tggtccacgg cggcctgctg
      961 ggcgtccgcg acgcggccga ccatgcgaag gccatggccg accacggcat cggcggcatc
     1021 gacctgctgt acgtcaacct ctacccgttc gaggccacgg tggccgctgg cggagacttc
     1081 gcgacctgcg tcgagaacat cgacatcggc ggcccggcga tgatccgctc ggcggccaag
     1141 aaccacggct atgtcgcggt ctgcaccgac ccggccgaca tggccgaggt gctgctgcag
     1201 ctgaaggacg gcgggaccac gacgctggac ctccgcaagc ggctggcggc ccgcgcctac
     1261 gcccgcacgg cggcctatga cgcggccatc ggggcctggt tcgccgacca gctgggtgac
     1321 accgctccgg cccgcaaggc catcgccggg accctgatcc agaccatgcg ctacggcgag
     1381 aacccgcacc agtcggcggc cttctacgcc ggcggcccgg cgcgaccggg cgtggccaac
     1441 gcccgccagc tgcagggcaa ggaactgagc tacaacaaca tcgccgacac cgacgcggcc
     1501 ttcgaactgg tcgccgagtt cagcgagccg gcctgcgtca tcgtcaagca cgccaacccc
     1561 tgcggcgtgg ccacgggcgg cgacctgctc ggcgcctacc agcgcgccct cgagtgcgac
     1621 ccggtcagcg ccttcggcgg catcgtcgcg gtcaaccgtc gcctcgaccg caaggccgcc
     1681 gaggccatcg tcgaggtgtt caccgaggtg gtcatcgccc ccgaggccga cgacgacgcc
     1741 gtggcggtgt tcgaggcgaa gaagaacctg cgcctgctgg tcaccggcgc cctgcccgat
     1801 ccgcgcgccc cgggcgaggt gttccgctcg gtcgccggcg gcttcctggt ccagagccgc
     1861 gacgcctcgc tgatcctgcc cggcgatctg aagatcgtca cgaaaagggc gccatcgccg
     1921 gccgagatca ccgacatgct gttcgccttc accatcgcca agcatgtgaa gtccaacgcc
     1981 gtggtgttcg cccgcggcgg ccagaccctg ggcgtcggcg ccggccagat gaaccgcaag
     2041 gacagcgccc gca
//
LOCUS       DAORNX010000517         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_40487_length_2051_cov_8.165832, whole genome shotgun sequence.
ACCESSION   DAORNX010000517 DAORNX010000000
VERSION     DAORNX010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40487_length_2051_cov_8.165832"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLO65_16045"
     CDS             <1..241
                     /locus_tag="PLO65_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29811.1"
                     /translation="ADTAGLNVAYAQLQGPGALRALRVSENERVRIIAEEELEAAWTG
                     KIPAKQALDNAVQRGNLVLSGASKAPAPAAPPRKK"
     gene            265..>974
                     /gene="ugpQ"
                     /locus_tag="PLO65_16050"
     CDS             265..>974
                     /gene="ugpQ"
                     /locus_tag="PLO65_16050"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF006989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRD29812.1"
                     /translation="MPHVFAHRCGGALAPENTLAGLRIAARLGLRAVEFDVMLSADAS
                     PWLLHDETLARTTNGAGRVCETSDAELSVLDAGIRQHPAFAGEPLPTLASAALVCRQL
                     GLLANVEIKPAAGFDALTGEVVARAVRALWAGAALPLLSSFSETALAAARAAVPELPL
                     GMLHERPPADWRERLRQLAAVTLHCRADCLDDELLGEARAHGIPVLCYTVNDPALASA
                     LHRRGVAAVFSDRIDCVAG"
     assembly_gap    975..1178
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1179..>1425
                     /locus_tag="PLO65_16055"
     CDS             <1179..>1425
                     /locus_tag="PLO65_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRD29813.1"
                     /translation="ADPTLLFTNAGMNQFKDVFLGFDKRPYLRATTAQKCVRAGGKHN
                     DLENVGYTARHHTFFEMLGNFSFGDYFKLDAIRYAWEL"
     assembly_gap    1426..1648
                     /estimated_length=223
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1649..>2051
                     /locus_tag="PLO65_16060"
     CDS             <1649..>2051
                     /locus_tag="PLO65_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRD29814.1"
                     /translation="GPPGSPDEEGDRYIEIWNNVFMQFNRDEAGVMHPLPKPSVDTGM
                     GLERTAAVLQHVHSNYEIDLFVRLIAAAARETSSADLDSPSLRVLADHIRACAFLISD
                     GVIPGNEGRGYVLRRIIRRAIRHGYKLGARAA"
BASE COUNT          247 a          564 c          548 g          265 t
ORIGIN      
        1 ggccgatacc gccggcctca atgtcgctta cgcgcagttg cagggaccag gcgcgctgcg
       61 tgcgctgcgc gtttccgaga acgagcgggt gcggatcatc gccgaggagg aactcgaagc
      121 cgcctggact ggcaagatac cggccaaaca ggcgctcgac aacgctgtcc agcggggcaa
      181 tctcgtcctc tccggagcgt caaaggcccc cgcaccggcg gcaccacccc ggaagaagtg
      241 attgctccac gcctcggctg gcgcttgccg cacgttttcg cgcaccgctg cgggggggcg
      301 ctggcgccgg agaacacgct ggccggtttg cgcatcgccg cccgcctggg cctgcgggcg
      361 gtcgaattcg acgtgatgct gtcggccgac gcctcgccgt ggctgctgca cgacgaaacg
      421 cttgcccgca cgacgaacgg cgccggccgg gtgtgcgaga cttccgatgc cgaactgtcg
      481 gtcctcgatg ccggcattcg ccagcacccg gcgtttgccg gcgaaccgct gccgacgctg
      541 gcctcggcag ccctcgtctg caggcaactg ggtctgctgg ccaatgtcga gatcaaaccg
      601 gccgccggtt tcgatgccct gaccggcgag gtcgtcgcgc gcgcggtgcg tgcgctgtgg
      661 gccggcgcgg cgctgccgct gctctcgtcg ttttccgaaa cggccctggc ggcggcgcgg
      721 gcggccgtcc cggaactgcc gctcggcatg cttcatgaac gtccgccggc ggactggcgg
      781 gaacggctgc ggcagctcgc ggcagtgacc ctgcattgtc gcgccgattg cctggacgac
      841 gagctgctgg gcgaagcgcg cgcgcatggc atcccggtgc tctgctacac ggtcaacgac
      901 ccggcgctgg cgagcgcgct ccatcggcgg ggggtggcgg cggtgttcag cgatcgcatc
      961 gattgcgtcg ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ccgatccgac gctgttgttc
     1201 accaacgccg gcatgaacca gttcaaggac gtttttctgg gtttcgacaa gcggccgtac
     1261 ctgcgcgcga ccaccgcgca gaaatgcgtt cgtgccggcg gcaagcacaa cgacctcgaa
     1321 aacgtcggct acaccgcgcg tcaccacacc tttttcgaaa tgctcggcaa tttctcgttc
     1381 ggcgactact tcaagctcga cgcgatccgc tacgcctggg aactgnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggccaccggg atcgcccgac gaagagggcg
     1681 accgttacat cgagatctgg aacaacgtct tcatgcagtt caaccgcgac gaggccggcg
     1741 tcatgcaccc gctgcccaag ccgtcggtcg ataccggcat ggggctggag cggacggcgg
     1801 cggtgctgca gcatgtccat agcaattacg agatcgacct gttcgtccgc ctgatcgctg
     1861 ccgccgcgcg cgagacgtcg tccgccgacc tcgacagccc ctcgctgcgg gtgctggccg
     1921 atcacatccg cgcctgcgcc ttcctgatct ccgacggggt gattcccggc aacgaggggc
     1981 ggggctatgt cctgcggcgc atcatccggc gcgccatccg gcacggctac aaactcggcg
     2041 cgcgggcagc c
//
LOCUS       DAORNX010000518         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_40489_length_2051_cov_4.009519, whole genome shotgun sequence.
ACCESSION   DAORNX010000518 DAORNX010000000
VERSION     DAORNX010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40489_length_2051_cov_4.009519"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLO65_16065"
     CDS             <1..359
                     /locus_tag="PLO65_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29815.1"
                     /translation="PAEVCSGPHPVAGVEIRGPVLHVIDGETICVALGYAPDEWIALR
                     LADAPVASPIRRVSSRAGADPRGVMMAAAFAKMAECRTELDRGGEVVAVCTVDGVPLA
                     RALGDGSVIAAGMNWR"
     gene            complement(425..1117)
                     /locus_tag="PLO65_16070"
     CDS             complement(425..1117)
                     /locus_tag="PLO65_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRD29816.1"
                     /translation="MRRADRLFQIIQILRRGRGPVTADAMAEELETSKRTIYRDIADL
                     MAQRVPIRGEAGVGYVLEGGFDLPPLMLTADEIEAAVLGAQWVASRGDPALSRAALDL
                     VAKISAAVPERLRPLILDPTTRSPPSNWRRPDGLDMARVRAAIHAGLKLQLHYRDEAE
                     RESRRTVWPIAVGYMDAVRLLVAWCELRGDFRSFRTDRVIEAAFLDERYPERPAILRA
                     KWRKTLGRPKAA"
     gene            complement(1204..1770)
                     /locus_tag="PLO65_16075"
     CDS             complement(1204..1770)
                     /locus_tag="PLO65_16075"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRD29817.1"
                     /translation="MPERPPERLVRSWLETPVATLQVVVDPEDVVRVLDFDNDSPRTR
                     RLMAIHYGGVPVVEGETPPAIRDALGRYFAGELAALADIPYRVAGTDFQRQVWRALTE
                     IPAGETRSYSQQAARIGRPAAVRAVGLANGANPVGIVIPCHRVIGSGGSLTGYGGGLE
                     RKRWLLAHEGAWGHVPPGTCPLIELRRV"
     gene            complement(1751..>2051)
                     /locus_tag="PLO65_16080"
     CDS             complement(1751..>2051)
                     /locus_tag="PLO65_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase 2"
                     /protein_id="HRD29818.1"
                     /translation="LFGPQASLEEAVARLRALPGIGEWTAQYIAMRQLREPDAFPAAD
                     IGLMRALEVEGVRPGPAELLARAEQWRPWRAYAALHLWASDSVALKAIADARAAA"
BASE COUNT          286 a          755 c          730 g          280 t
ORIGIN      
        1 cgccggccga ggtctgcagc gggccgcacc cggtcgccgg cgtcgagatc cgcggaccgg
       61 tcctgcatgt catcgacggc gagaccatct gtgtcgcgct cggctacgcg ccggacgagt
      121 ggatcgccct gcggctggcc gatgcgccgg tcgcctcacc catccggcgg gtctccagcc
      181 gcgccggggc cgaccccagg ggcgtgatga tggccgccgc cttcgccaag atggccgagt
      241 gccggaccga actggatcgg ggtggcgagg tcgtcgcggt ctgcaccgtc gacggcgtgc
      301 cgctggcgcg cgccctcggt gacggatcgg tcatcgcggc ggggatgaac tggcggtagg
      361 gcggcccacg ggaaacccct gccgcagggt ccccccctcc caagggggag agaacggccg
      421 gtgatcacgc cgccttcggc ctgcccagcg tcttgcgcca cttcgcccgc aggatcgccg
      481 gccgttccgg atagcgctcg tcgaggaacg ccgcctcgat cacccggtcg gtgcggaagc
      541 tgcggaagtc cccgcgcagt tcgcaccagg cgaccagcag ccgcaccgca tccatgtagc
      601 ccacggcgat cggccagacc gtgcggcgcg actcccgctc ggcctcgtcg cgatagtgca
      661 gctgcagctt gaggcccgcg tggatcgcgg cccgcacgcg cgccatgtcc agcccgtcgg
      721 gccggcgcca gttgctcggc gggctgcggg tggtggggtc gaggatcagc ggccgcagcc
      781 gctcgggcac cgccgcgctg atcttggcca ccaggtccag cgccgcccgc gacagggccg
      841 ggtcgccgcg gctggcgacc cattgcgcgc cgagcaccgc cgcctcgatc tcgtcggccg
      901 tcagcatcag cggcggcagg tcgaagccgc cctccagcac atagccgacg ccggcctcgc
      961 cgcggatcgg cacccgctgg gccatcaggt cggcgatatc gcggtagatc gtgcgtttgg
     1021 aggtctccag ctcctcggcc atggcgtcgg ccgtcaccgg cccgcgcccg cggcgcagga
     1081 tctggatgat ctggaacagg cggtcggcgc gtctcatggc ggggttctag ccgggctctg
     1141 ctgacagcat gatggcagca ggttggcggg agcaagggga catgtaccaa aggtacgtgt
     1201 cccctaaacc cgtcgcagct cgattagggg acacgtacct ggaggtacat gtccccaagc
     1261 cccctcatgc gccagcagcc agcgcttgcg ctcgagccca ccgccgtagc cggtcaggga
     1321 tccgcccgag ccgatcaccc tgtggcaggg aatgacaatg ccgaccggat tggcgccgtt
     1381 ggccaggccc accgcgcgca ccgccgccgg ccggccgatc cgggcggcct gctggctgta
     1441 ggaacgggtc tctccggccg gaatttcggt cagcgcccgc cagacctggc gctggaaatc
     1501 cgttcccgcc acgcgatacg gaatgtcggc cagggccgcg agctcacccg cgaagtaacg
     1561 gcccagggcg tcgcggatcg ccggcggcgt ctcgccctcg acgaccggga cgccgccgta
     1621 gtggatcgcc atcagtcgcc gggtgcgcgg gctgtcgttg tcgaaatcca gcacccgcac
     1681 gacgtcctcc ggatcgacga cgacctgcag cgtcgcgacc ggcgtctcca gccagcttcg
     1741 gacaaggcgt tcaggcggcc gctcgggcat cggcgatcgc tttcagggcc acggagtcgg
     1801 acgcccacag atgcagggcg gcgtaggccc gccacggccg ccactgttcg gcccgggcca
     1861 gcagctcggc gggaccgggc cgcacgccct cgacttccag cgcgcgcatc agcccgatgt
     1921 cggcggccgg gaaggcgtcg ggctcgcgca gctggcgcat ggcgatgtac tgcgccgtcc
     1981 attcgccgat ccccggcagg gcgcggagtc tggccacggc ctcctccagc gaggcctgcg
     2041 gcccgaacag g
//
LOCUS       DAORNX010000519         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41042_length_2028_cov_6.015712, whole genome shotgun sequence.
ACCESSION   DAORNX010000519 DAORNX010000000
VERSION     DAORNX010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41042_length_2028_cov_6.015712"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..471)
                     /locus_tag="PLO65_16085"
     CDS             complement(61..471)
                     /locus_tag="PLO65_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRD29819.1"
                     /translation="MTKASEADTPARLIDARIAALGDWRGETLARVRALIHEADPEVV
                     ETWKWRGVPVWEHGGILCTGETYRAVVKLTFARGAALDDPAGLFNASLEGNTRRAIDI
                     REGDRIDAAAFKALIRAAVALNTAPRARKASSGL"
     gene            complement(503..922)
                     /locus_tag="PLO65_16090"
     CDS             complement(503..922)
                     /locus_tag="PLO65_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRD29820.1"
                     /translation="MAEPVLLSGGNPQIPKGDGDGPVQAYIAAMPGWKREVGRRLDAL
                     ITGTVPGVRKAVRWNSPFYGVDGPDGDKGWFLSFHCFNRYVKVTFFRGAALDPVPPGA
                     SKQADVRYLDIHEDAPLDAAGFADWVRQASRLPGVRL"
     gene            complement(933..1364)
                     /locus_tag="PLO65_16095"
     CDS             complement(933..1364)
                     /locus_tag="PLO65_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD29821.1"
                     /translation="MTAATTDTRSVIVERDFAHQPEKLWRALTQPHLIEAWLMKNDFA
                     PAIGHRFNLRGEWGGVLDCEVLALEPPRRLAFSWDFVHEDPAFDLRSVVTFTLTPTAT
                     GTHLRVEQAGFRPGQKQALGGALHGWRGFLDALDPVLNRLD"
     gene            complement(1361..1684)
                     /locus_tag="PLO65_16100"
     CDS             complement(1361..1684)
                     /locus_tag="PLO65_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD29822.1"
                     /translation="MPTPQDLLFRTLADPTRRAIFERLCREGEQTVGALTARAGVSQP
                     AVSKHLGALKLAGLVRDRPAGRQTHYSARPDALAPLDDWTREMAGFWRSRLDGLEDLL
                     NRMDQ"
     gene            complement(1760..>2028)
                     /locus_tag="PLO65_16105"
     CDS             complement(1760..>2028)
                     /locus_tag="PLO65_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--alanine ligase"
                     /protein_id="HRD29823.1"
                     /translation="LEGLSEDDVLLMPEPVYFGGTTIREVTSEDIAADIRAGGRTAEA
                     LSDRPACGDRLVALARPGDRIIIMGARDDTLSLFAAEVLERLPA"
BASE COUNT          284 a          710 c          727 g          307 t
ORIGIN      
        1 ggtcaaggtc ccgatccggt tcggcctggc gccggaggag cccgccgggc cggccagccc
       61 ctagaggccg cttgacgcct tccgcgcgcg cggcgccgtg ttcagcgcca ccgcggcccg
      121 gatcagcgcc ttgaaggccg ccgcgtcgat ccggtcgccc tcgcggatgt cgatcgcccg
      181 ccgggtgttt ccctcgaggc tggcattgaa caggccggcg gggtcgtcca gcgccgcgcc
      241 gcgggcgaag gtcagcttga ccaccgccct gtaggtctcg ccggtgcaga ggatgccgcc
      301 gtgctcccac accggaacgc cgcgccactt ccacgtctcg accacctcgg gatcggcctc
      361 gtggatcagg gcccggaccc gggcgagtgt ctcgccccgc cagtcgccca gcgcggcgat
      421 ccgcgcatcg atcagccggg ccggcgtgtc ggcttcgctg gctttggtca tgcggcactc
      481 cctttcgtcg gactcgcaac ggtcacaggc ggacgcccgg caggcggctc gcctgccgca
      541 cccagtcggc gaaccccgcc gcgtcgaggg gcgcgtcctc gtgaatgtcg aggtagcgca
      601 cgtccgcctg tttcgacgcg cccggcggga ccgggtcgag cgccgcgccg cggaagaagg
      661 tcaccttcac gtagcggttg aagcagtgga agctgaggaa ccagcccttg tcgccgtccg
      721 ggccgtcgac gccatagaag ggcgagttcc atctgaccgc cttgcgcacg ccggggacgg
      781 tcccggtgat cagcgcgtcg aggcggcgac cgacctcacg cttccagccc ggcatggcgg
      841 cgatataggc ctgcaccggt ccgtcgccgt cccccttggg gatctgcggg ttgccgcctg
      901 agaggagcac cggttcggcc atggctgttt cctcagtcga gcctgttcag caccgggtcg
      961 agggcgtcga ggaagccgcg ccagccgtga agcgcgccgc cgagggcctg cttctggccg
     1021 ggccggaagc cggcctgctc gacccgcaga tgcgtgcccg tggcggtcgg cgtcagggtg
     1081 aaggtgacca cgctgcgcag gtcgaaggcc gggtcctcgt ggacgaaatc ccagctgaag
     1141 gccaggcggc ggggcggctc gagggcaagc acctcgcagt ccagcacgcc gccccattcg
     1201 ccgcgcaggt tgaagcggtg gccgatcgcc ggcgcgaaat cgttcttcat cagccaggcc
     1261 tcgatcaggt gcggctgcgt cagggcccgc cagagcttct ccggctgatg ggcgaagtcc
     1321 cgctcgacga tcacggagcg ggtgtcggtc gtggcggcgg tcattgatcc atcctgttca
     1381 gcaggtcctc gaggccgtcg aggcggctcc gccagaagcc ggccatctcc cgggtccagt
     1441 cgtccagggg ggccagcgcg tcgggccggg cgctgtagtg ggtctgccgg cctgcgggac
     1501 ggtcgcgcac cagcccggcc agcttcagcg cgcccaggtg tttcgagacc gccggctgcg
     1561 agactccggc gcgggccgtc agggccccga ccgtctgttc gccctcgcgg cacagccgct
     1621 cgaagatcgc ccgacgggtc gggtcggcca gggttcggaa cagcagatcc tgcggcgtgg
     1681 gcatggggaa tcgataatcc gtcagctatg aattactcat aacttcagaa ttatgtatcg
     1741 gtcaagcagc ggtgagccgt caggccggca gccgttccag gacctcggcc gcgaacagcg
     1801 acagggtgtc gtcgcgggcg cccatgatga tgatgcggtc accgggccgg gcgagcgcga
     1861 ccagccggtc gccgcaggcc gggcggtcgg agagggcttc ggctgtgcgg cctccggcgc
     1921 ggatatcggc ggcgatgtcc tcgctggtca cctcgcggat cgtcgtaccg ccgaaataga
     1981 ccggctccgg catcagcagc acgtcgtcct ccgaaagacc ctccagga
//
LOCUS       DAORNX010000520         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41072_length_2027_cov_8.017748, whole genome shotgun sequence.
ACCESSION   DAORNX010000520 DAORNX010000000
VERSION     DAORNX010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41072_length_2027_cov_8.017748"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PLO65_16110"
     CDS             complement(<1..1515)
                     /locus_tag="PLO65_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRD29824.1"
                     /translation="MRHDNVTLDDKYLLEDGRAFITGVQALIRVMLDRRRLDQRDGLN
                     TAGFVSGYRGSPLGGLDQQAARARKLLTSHEIVFKEGLNEDLAATAVWGSQQANLFPG
                     AKYDGVSAMWYGKAPGVDRTGDVFKHANFAGTTPKGGVIAVAGDDHNCKSSTLPSQSE
                     YAFQDFEMPVLSPADVQEVLDYGLMGLSMSRFSGLWVGLIALADTMDSGVTIDVSLDR
                     HRHVLPENFAMPPGGLHIRLKDQPMDKELRLRNYRIPAALAFARANGIDRVALAAARP
                     RLGIVCSGQAYKDVLEAFQAMGLGLDEAAALGVSIYKVGMPWPLEPQGLRAFAAGLET
                     LMVIEHKRPLIEVQARAALYDLPAHARPKIIGKKDENGQPLMEETGSLTVAAIALDLY
                     NQLPAGPHRERAQAYLDKVSAASMAAVTLASDQQRKPFFCSGCPHNTSTRLPEGSRAL
                     AGIGCHYMASFNDPSTDLNTHMGGEGLTWIGAAPFTSEKHVFQNLGDGTYNHSGS"
     gene            1658..>2027
                     /locus_tag="PLO65_16115"
     CDS             1658..>2027
                     /locus_tag="PLO65_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD29825.1"
                     /translation="MAEQLDAVDAKILDLIQHDASLSVAEIAERVGLSSSPCWRRIKR
                     LEDAGIIQRRVTILDREQLGLGFEVYCTVKLSLPTKENLELFETNVVKWAEVVQCATV
                     TGAADYELRIVTRDMHAFDDF"
BASE COUNT          324 a          653 c          707 g          343 t
ORIGIN      
        1 cgagccggag tggttgtagg tgccgtcgcc gaggttctgg aagacgtgct tctcgctggt
       61 gaacggcgcc gcgccgatcc aggtcaggcc ctcgccgccc atgtgggtgt tcaggtcggt
      121 cgaggggtcg ttgaagctgg ccatgtagtg gcagccgatg ccggccaggg cgcgactgcc
      181 ctcgggcagg cgggtcgagg tgttgtgcgg gcagcccgag cagaaaaacg gcttgcgctg
      241 ctggtcgctg gccagggtga cggcggccat cgaggcggcc gacaccttgt ccagataggc
      301 ctgggcccgc tcgcggtgag gcccggccgg cagctggttg tacaggtcga gggcgatggc
      361 ggcgacagtc agggagccgg tctcctccat caacggctgg ccgttctcgt ccttcttgcc
      421 gatgatcttc ggccgcgcat gggccggcag gtcgtagagg gcggcgcgcg cctggacctc
      481 gatcagcggc cgcttgtgct cgatgaccat cagggtctcg aggccggcgg cgaaggcgcg
      541 caggccctgc ggctccagcg gccagggcat gccgaccttg tagatcgaca cgccgagggc
      601 ggcggcctcg tcgaggccca gccccatggc ctggaaggcc tccagcacgt ccttgtaggc
      661 ctgacccgag cagacgatgc ccaggcgcgg ccgggccgcc gccagggcca cccggtcgat
      721 gccgttggcg cgggcgaagg ccagggcggc gggaatgcgg tagttgcgga gccgcagctc
      781 cttgtccatc ggctggtcct tcagccggat gtgcagaccg ccgggcggca tggcgaaatt
      841 ctcgggcagg acatgccggt ggcggtcgag cgacacgtcg atcgtcacgc cgctgtccat
      901 ggtgtcggcc agggcgatca gcccgaccca caggccgctg aagcgggaca tcgacaggcc
      961 catcaggccg tagtcgagca cctcctgcac atcggccggc gacagcaccg gcatctcgaa
     1021 gtcctggaag gcgtattcgg attgcgacgg cagggtcgag gacttgcagt tgtggtcgtc
     1081 gccggcgaca gcgatcacgc cgcccttcgg ggtcgtcccg gcgaagttgg cgtgcttgaa
     1141 cacgtcgccg gtgcggtcga cgccgggggc cttgccgtac cacatggcgc tgacgccatc
     1201 gtacttcgcg cccgggaaaa ggttggcttg ctggctgccc cagacggccg tcgcggcgag
     1261 gtcctcgttg aggccttcct tgaagacgat ctcgtggctg gtcagcagct tccgggcccg
     1321 ggccgcctgc tggtccagcc cgccgagcgg cgagccgcga tagccggaca cgaatccggc
     1381 ggtattcaat ccgtcgcgct ggtcgaggcg tcggcgatcc agcatcaccc ggatcaaggc
     1441 ctgcacaccg gttatgaacg cgcggccgtc ttcgagcaga tatttgtcgt cgagcgtcac
     1501 attgtcgtgg cgcattgaaa aatctttcct ttggagggcg cggttcggct tgcaagatca
     1561 tataagcgcg gcgaaattgc ttgccaaacc cgtgcccctt cccctgcccc tcacggcaag
     1621 atcggcgcgc ggatcggcat attaggggga aaaaggtttg gccgagcagc tagacgcggt
     1681 tgacgcaaag attctcgatc tcatccagca tgacgccagc ctgtcggtcg ccgaaatcgc
     1741 cgaacgggtc ggtctgtcgt ccagcccttg ctggcggcgg atcaagcggc tggaagacgc
     1801 cggcatcatc cagcgccggg tgaccatcct cgaccgggaa cagctgggcc tcggcttcga
     1861 ggtttactgc accgtcaagc tctccctgcc gaccaaggag aacctcgagc tgttcgagac
     1921 caacgtggtc aaatgggccg aggtcgtgca atgcgccacc gtcaccggcg cggccgacta
     1981 cgaactgcgc atcgtcaccc gcgacatgca cgccttcgac gacttcc
//
LOCUS       DAORNX010000521         2025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41122_length_2025_cov_6.556853, whole genome shotgun sequence.
ACCESSION   DAORNX010000521 DAORNX010000000
VERSION     DAORNX010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41122_length_2025_cov_6.556853"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLO65_16120"
     CDS             complement(<1..337)
                     /locus_tag="PLO65_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD29826.1"
                     /translation="MRAIAKTGLGRLPGLALRALLAVAITGLPASAVTVPPPAAARPN
                     IVMIVVDDAALMDFGAFGGEARTPNIDRLARSGAMFTAYHTSPLCSPSRAMLLTGVDN
                     HRAGVATIEE"
     gene            561..1052
                     /locus_tag="PLO65_16125"
     CDS             561..1052
                     /locus_tag="PLO65_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29827.1"
                     /translation="MRDVSNETERHNRRAARLRFIRQLHLWVAVFVAPSLMLFAVTGM
                     LQLFKLHEAEGGYRPPPVIEKLGQVHIHQKYALRPVRRPSGAQPAPAASAPARGEAEA
                     EEPAAPLGETLTRWTFLVMSLGMAMSAVLGVWMSLSNTRQRRTTWLLLAAGTVLPILV
                     LAL"
     gene            complement(1208..>2025)
                     /locus_tag="PLO65_16130"
     CDS             complement(1208..>2025)
                     /locus_tag="PLO65_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD29828.1"
                     /translation="LAGQAVVADGGGAAAALTGVISGVKRLGDRMAIQDPAQLSTIRA
                     RLIQAGWFSREAVAVYLGAKAVALLLATLATILTLPWAISGGGGMGAILLAGGFAVAA
                     LLGPDQVIKARRRKLELEYMDGFPDLLDLLVASVEAGLSLDAAVNRVGEELARRYVHL
                     AEHLRFLTLELRAGRSRRDAWSSFAERLGIDDARALATMLRQSEEMGTSLGDTLTVFS
                     EDMRAKRMLRAEEKAMSLPAKLMVPLILFIFPCLMGVLILPAAYRISQTLMAN"
BASE COUNT          315 a          710 c          709 g          291 t
ORIGIN      
        1 tctcctcgat cgtcgccacg ccggcccggt ggttgtcgac gccggtcagc agcatggccc
       61 gcgacggcga gcagagcggc gaggtgtggt aggcggtgaa catcgcgccg gaccgcgcca
      121 gccggtcgat gttgggcgtc cgcgcctcgc cgccgaacgc tccgaagtcc atcagggccg
      181 catcgtcgac cacgatcatc acgatgttgg gtcgggccgc cgctggcggc gggacggtca
      241 ccgccgacgc gggaaggccg gtgatggcga cggccaggag ggccctcagc gccaggcccg
      301 gcagtcggcc gaggcccgtt ttggcgattg ctcgcaagat caccctcccc agcgtcggcg
      361 ccggtggcgg cgcagtgagt tacccaagag taaccagaca tcggcgacgg ggtcaaacca
      421 ggcctccgac ggtcgcgcgg cgcgagacgc ctttggaatt gatcggtgtc atggctcgcg
      481 tcgggtccgc gcgtctatcc acgcgccggg atgccggatt gctcgcagtc cgcggtgatc
      541 ggccatccgg agtagggccc gtgagagacg tgtcgaacga gaccgagcgc cacaatcgcc
      601 gcgcagcccg gctgcggttc attcgccagc tgcatctgtg ggtcgcggtg ttcgtcgccc
      661 cgagcctgat gctgttcgcc gtcacgggca tgctgcagct gttcaagctc cacgaggccg
      721 agggcggcta ccggccgccg ccggtgatcg aaaagctcgg ccaggttcac atccaccaga
      781 aatacgcgct tcggcctgtc cgccgcccgt cgggcgcgca acccgcgccg gccgccagcg
      841 cgccggcaag gggcgaagcc gaagccgaag agccggcggc gcctctcggc gagacgctga
      901 cgaggtggac cttcctggtc atgtccctcg gcatggctat gtcggccgtg ctgggtgtct
      961 ggatgagcct gagcaatacg cgccagcgcc ggaccacctg gctgctgctg gcggccggaa
     1021 ccgtgctgcc gatcctcgtg ctggcgctct agaacacgac gccccctgcc gtcatcgtcg
     1081 gacctgttcc gacgacccat gaacgcggcg cctcccagtg aggactcccc gttcagcgcg
     1141 tgcatgggtt ctcgcgacaa gcgcgaggtt gacgtgaaaa gggcgcacgg accggcgcgc
     1201 cagcgctcta gttggccatc agggtctggc tgatgcgata ggccgccggc aggatcagca
     1261 cccccatcag gcaggggaag atgaacagga tcagcggcac catcagcttg gccggcaggc
     1321 tcatggcctt ttcctcggcc cgcagcatgc gcttggcgcg catgtcttcg gagaagacgg
     1381 tcagggtgtc gcccaggctc gtgcccattt cctcggactg acgcaacatc gtcgccaggg
     1441 cgcgggcgtc gtcgatgccc agccgctcgg cgaagctcga ccaggcgtcg cgccgcgagc
     1501 gacccgcgcg cagttcgagg gtcaggaagc gcaggtgttc agccaggtgg acgtaacgcc
     1561 gcgcgagttc ctcgccgacc cggttgacgg cggcgtccag cgataggccg gcctcgaccg
     1621 acgccaccag caggtccaga aggtcaggaa agccgtccat atactcgagc tcgagcttgc
     1681 gccgacgggc cttgatcacc tggtcgggcc cgagcagcgc cgccacggcg aagccgccgg
     1741 ccagcaggat cgcccccatg ccgcccccgc cgctgatcgc ccagggcaag gtcaggatgg
     1801 tcgccagggt ggccagcagc agcgccacgg ccttggcgcc gagatagacg gcgacggcct
     1861 cccggctgaa ccagccggcc tggatcagcc tggcgcggat ggtcgagagc tgggccggat
     1921 cctggatggc catccggtca cccagccgct tgacgccgct gatcacgccg gtcagggccg
     1981 cggccgcgcc gcccccgtcc gcgacgacgg cctggcccgc caggc
//
LOCUS       DAORNX010000522         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41193_length_2022_cov_5.420946, whole genome shotgun sequence.
ACCESSION   DAORNX010000522 DAORNX010000000
VERSION     DAORNX010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41193_length_2022_cov_5.420946"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLO65_16135"
     CDS             complement(<1..977)
                     /locus_tag="PLO65_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRD29829.1"
                     /translation="MTEADAPAQPADRPLTQDGPPVRLYLIDGSGYIFRAYHALPPLT
                     RKSDGLPVGAVQGFCNMLFKLLRDMKEGGPTHLAVIFDHSDKTFRNTLYDQYKAHRPP
                     LPEDLVPQFPLVREATRAFGVPSIELAGYEADDLIAAYSCKVRDAGGEVVIVSSDKDL
                     MQLVGERVSMLDTMKNVLIGPDQVFEKFGVGPDKVVEVQALCGDSVDNVPGAPGIGVK
                     TAALLINEYGDLETLLSRAHEIKQEKRRETLINFADQIRLSRQLVQLDCDTPLPEPVE
                     DLAVREPDGPTLAAFLEQMEFRGLARRVGDAAAGNGMAPAPMPKMGAPRP"
     gene            1076..>2022
                     /locus_tag="PLO65_16140"
     CDS             1076..>2022
                     /locus_tag="PLO65_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRD29830.1"
                     /translation="MRLALLGLGLWALASGAQGQTVVNPDWARRPTGDEIARYYPDEA
                     LDKSVEGRVTIECSVAISGALKDCEVLSESPAGVGFGRAGVRMAQAVFRMTPRMVDGK
                     PDDGARIKIPLHFAAATFSARAIILDAVWDSAPSFEDMAAAWPASAGDIPVGTSLLRC
                     TVGSQADLRNCTIVGQTPKGSPFGGAARSLVGKFKLKITPGDLEKYAFADIAVSFLFY
                     NPATPAGQARKVEKPDWIARINPDKVVALYPAQAADAGVREGVGVADCLVAPDGRLTD
                     CRVAREAPADLGFGQAAVLAVSVMRMDPWTPEGRPVAGAR"
BASE COUNT          286 a          669 c          738 g          329 t
ORIGIN      
        1 ggcctcggcg cgcccatctt cggcatcggc gccggggcca tgccgttgcc ggccgccgcg
       61 tcgccgaccc gccgggccag accgcggaac tccatctgct cgaggaaggc ggccagggtc
      121 gggccgtccg gctcgcgcac cgccaggtcc tcgaccggct cgggcagggg cgtgtcgcag
      181 tcgagctgca cgagctgccg cgacaggcgg atctggtcgg cgaagtttat cagcgtctcg
      241 cggcgcttct cctgcttgat ctcgtgggcc cggctgagca acgtctccag gtcgccgtac
      301 tcgttgatca gcagggccgc cgtcttcacg ccgatgcccg gggcgccggg cacgttgtcg
      361 acgctgtcgc cgcacagggc ctggacctcg accaccttgt cgggccccac gccgaatttc
      421 tcgaacacct ggtcggggcc gatcagcacg ttcttcatcg tgtcgagcat cgacacccgc
      481 tcgccgacca gctgcatcag gtccttgtcg gacgagacga tgaccacctc gccgccggcg
      541 tcgcgcacct tgcagctgta ggcggcgatc aggtcgtcgg cctcgtagcc ggccagctcg
      601 atgctcggca cgccgaacgc ccgggtcgcc tcgcggacca gcgggaattg cgggaccagg
      661 tcctctggca gcggcgggcg gtgggccttg tactggtcgt agagggtgtt gcggaaggtc
      721 ttgtcggagt ggtcgaagat caccgccagg tgggtcgggc cgccctcctt catgtcccgc
      781 agcagcttga acagcatgtt gcagaagccc tgcaccgccc ccaccggcag gccgtcagac
      841 ttgcgcgtca gcggcggcag ggcgtggtag gcgcggaaga tgtagcccga accgtcgatc
      901 agatagagcc gcacaggggg gccatcctgg gtcagggggc ggtcggcggg ctgggcgggg
      961 gcgtcggctt cggtcatggg cggaagatag tgggcccggg cccggtggtc atcccgcgtt
     1021 tggcgccttg cggcgccgcc accgggtgct accttgaggt gtgaaggggg tggccatgcg
     1081 tctagcgttg ttgggtctgg gcctgtgggc gctcgcgtcc ggagcccagg gacagacagt
     1141 cgtcaatcct gactgggcgc gtcgcccgac gggcgacgag atcgcgcgct actatcccga
     1201 tgaggccctc gacaagagcg tggaggggcg ggtgacgatc gagtgttcgg tcgccatctc
     1261 cggcgcgttg aaggattgcg aagtgctgtc cgaatcgccc gctggcgttg gcttcggcag
     1321 ggcgggcgtc cggatggcgc aggccgtctt ccgtatgacg cctcggatgg tggacggcaa
     1381 acccgacgac ggcgccagga tcaagatccc actccacttc gccgcggcca cgttttcagc
     1441 gcgcgccatc atcctcgacg ccgtctggga ctcggcgccg agtttcgagg acatggcggc
     1501 ggcgtggccc gcgtcggcag gcgatattcc ggtcggaacg tcgctactgc gatgcaccgt
     1561 cggcagccag gccgatctgc ggaactgcac gatcgtgggg cagacgccga aaggatcgcc
     1621 gttcggcggc gcggcccgca gcctggtcgg caagttcaag ctcaagatca cacccgggga
     1681 tctggaaaag tatgccttcg ccgacatcgc cgtcagcttc cttttctaca atcccgccac
     1741 ccccgcgggg caggcgagga aggttgagaa gcccgactgg atcgcccgca tcaatccgga
     1801 caaggtcgtc gccctctatc cagcccaggc ggccgacgcc ggggtcaggg aaggggtcgg
     1861 ggtggctgat tgcctggtcg cgcccgacgg ccggctgacc gactgccggg tggcccgcga
     1921 ggcgccggcc gacctcggct ttggccaggc ggccgtgctg gcggtcagtg tgatgcggat
     1981 ggacccctgg acgccggagg gccggccggt cgcgggcgcg cg
//
LOCUS       DAORNX010000523         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41378_length_2015_cov_6.716837, whole genome shotgun sequence.
ACCESSION   DAORNX010000523 DAORNX010000000
VERSION     DAORNX010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41378_length_2015_cov_6.716837"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLO65_16145"
     CDS             <1..270
                     /locus_tag="PLO65_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD29831.1"
                     /translation="EGGTVTVIHRADRLGDLLALLGEKAGSFRVRPVQPFADAPAKRV
                     LVRAIKTGKAPLVLLPPLVLHDREGGKHSAAAEAILRGETALDWT"
     gene            complement(359..925)
                     /locus_tag="PLO65_16150"
     CDS             complement(359..925)
                     /locus_tag="PLO65_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HRD29832.1"
                     /translation="MRRIETLYLAGPEVWFPNVGAHFALRQAMCEAVGFSMLQADTSE
                     FRELEPGEVMAREIYAERVARMRRADAAIVNLTPWRGPTADPGTAFEAGFLSGLGKPV
                     MAYMNVAYEHEAEFRDRVDAHIGAQLDEDGVWRDGEGCLIEDFGLPETIMLWAEARRL
                     FVIVTPDVLGDVTGLQLCLDALRQYADD"
     gene            complement(934..1518)
                     /locus_tag="PLO65_16155"
     CDS             complement(934..1518)
                     /locus_tag="PLO65_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRD29833.1"
                     /translation="MKRLILAAVLALTGAPALAAPVAYTLDPAHTQTTFTVDRFGFTT
                     ILGAFTTASGTIWIDEADPANSRVEAQVTVASLYAGDATREEHLKGERWLNAAAYPAI
                     SFKSTKVVTTGPDTADVTGDFTLNGVTRAVTFKVKLNRIGKAPGSGKQAAGFTITGSV
                     HRRDFNVGTALPAGLIGEDVPIRIEALAVAAPAP"
     gene            complement(1547..>2015)
                     /locus_tag="PLO65_16160"
     CDS             complement(1547..>2015)
                     /locus_tag="PLO65_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29834.1"
                     /translation="LTLDRGEESGVSFNLSGGLVGLLVWALTAPALAASAAPGLPTEV
                     EGVVVVADTDVRVVVAVGGDIGETTYVASAPPGLACGGAQYQYMSRENRQCWLRVRRK
                     TPLILTAQASGHYGVDWTVQWVGCAPIGNGAACTLNAQDESQVAALFTRAPAS"
BASE COUNT          292 a          701 c          734 g          288 t
ORIGIN      
        1 gagggcggga ccgtcacggt gatccaccgc gccgaccggc tgggcgacct gctggcgctg
       61 ctcggcgaaa aggccggctc gttccgcgtg cgcccggtcc agccgttcgc cgacgcgccg
      121 gcaaagcggg tgctggtccg cgcgatcaag accggcaagg cgccgctggt gctgctgccg
      181 ccgctggtgc tgcacgaccg cgagggtggc aagcactcgg cggccgccga ggcgatcctg
      241 cggggggaga cggcgctgga ttggacgtag gggacatgta ccgccttcgg tacgtgtccc
      301 ccaatcaaat tggctgcggg atttagggga cacgtacctg gggtacatgt ccccgcgctc
      361 aatcgtccgc gtattgtcgc agcgcgtcga ggcacagctg cagaccggtc acgtcgccca
      421 gcacgtcggg cgtgacgatg acgaacagcc ggcgcgcctc ggcccacagc atgatcgtct
      481 cgggcaggcc gaagtcctcg atcaggcagc cctcgccgtc gcgccagacg ccgtcctcgt
      541 ccagctgggc gccgatgtgg gcgtcgacgc ggtcgcgaaa ctcggcctcg tgctcatagg
      601 cgacgttcat gtaggccatc accggcttgc ccaggcccga caggaagccg gcctcgaagg
      661 ccgtgcccgg atcggccgtc ggcccgcgcc agggggtcag gttgacgatc gccgcgtccg
      721 cccggcgcat gcgcgccacc cgctcggcgt agatctcccg ggccatgacc tcgcccggct
      781 cgagctcgcg gaactcgctg gtgtcggcct gcagcatgga gaagccgacg gcctcgcaca
      841 tggcctggcg cagcgcgaaa tgggccccga cattggggaa ccagacctcg ggtccggcca
      901 gatagagggt ttcgatgcgt cgcatgcccg cttctacggc gcgggcgcgg ccacggcgag
      961 ggcctcgatg cggatcggca cgtcctcgcc gatcaacccg gccggcagcg ccgtcccgac
     1021 gttgaagtcg cggcgatgca ccgagccggt gatggtgaag cccgcggcct gcttgccgga
     1081 gccgggcgcc ttgccgatcc tgttcagctt gaccttgaag gtcaccgccc tggtcacgcc
     1141 gttgagcgtg aagtcacccg tcacgtcggc ggtgtcggga ccggtggtga cgaccttcgt
     1201 cgacttgaag ctgatcgcag ggtaggcggc ggcgttcagc cagcgctcgc ccttgaggtg
     1261 ctcctcacgg gtggcgtcgc cggcgtagag gctggcgacc gtgacctggg cctcgacgcg
     1321 ggagttggcc gggtccgcct cgtcgatcca gatcgtgccg gacgcggtgg tgaaggcgcc
     1381 aaggatggtg gtgaagccga accggtcgac cgtgaaggtc gtctgggtgt gcgccgggtc
     1441 gagggtgtag gccaccggcg cggccagcgc cggggcgccg gtcagggcca ggacggcggc
     1501 gaggatcagg cgtttcatgg acaggctccg cgacaggccc tggagcctac gacgccggcg
     1561 cgcgcgtgaa cagcgccgcc acctgggact cgtcctgggc gttcagggtg caggccgcgc
     1621 cgttgccgat cggcgcgcaa ccgacccact gcacggtcca gtccaccccg tagtggccgc
     1681 tggcctgggc ggtcaggatc agcggggtct tgcgccgcac gcgcagccag cactgccggt
     1741 tctcgcggct catgtactgg tactgcgcgc cgccgcaggc caggccgggc ggcgcgctgg
     1801 cgacgtaggt ggtctcgccg atgtcaccgc cgaccgcgac caccacccgc acgtcggtgt
     1861 cggcgacgac gaccaccccc tcgacctcgg tcgggagacc gggcgcggcg ctcgccgcca
     1921 gcgccggagc ggtcagggcc cagacaagca ggccgaccag gccccccgac aggttgaacg
     1981 acacgccaga ctcctcgcca cgatccagag tcaga
//
LOCUS       DAORNX010000524         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41523_length_2009_cov_11.601842, whole genome shotgun
            sequence.
ACCESSION   DAORNX010000524 DAORNX010000000
VERSION     DAORNX010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41523_length_2009_cov_11.601842"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2009
                     /gene="rpoB"
                     /locus_tag="PLO65_16165"
     CDS             <1..>2009
                     /gene="rpoB"
                     /locus_tag="PLO65_16165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRD29835.1"
                     /translation="VSYEFEEPKYDVEECIQRDMTYAAPLKVKLRLIVFEMEEETGAR
                     SVKDIKEQDVYMGDIPLMTDKGTFIVNGTERVIVSQMHRSPGVFFDHDKGKTHSSGKL
                     LFAARVIPYRGSWLDFEFDAKDIVYVRIDRRRKLPATTFLYGLGMDGEEILTTFYDVV
                     PFEKREEKKGKPGGWATPYKPERWRGVKPEFALIDADTGEEVAAAGAKISARAAKKLA
                     DNGLKTLLLSPDALVGRYLARDEVNPETGEIYAEAGDELDTAIIEVLEEKGFTTIDVL
                     DIDHVTVGAYMRNTLRVDKNAVREDALFDIYRVMRPGEPPTVEAAEAMFKSLFFDQER
                     YDLSSVGRVKMNMRLEQDVADDVRVLRKEDVLEVLKVLVGLRDGRGEIDDIDNLGNRR
                     VRSVGELLENQYRVGLLRMERAIKERMSSVDIDTVMPHDLINAKPAAAAVREFFGSSQ
                     LSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPN
                     IGLINSLATHARVNKYGFIESPYRRVTDGKPQAEVVYMSAMEEAKHVIAQANIRMDGG
                     SIAEDLVPGRVNGEPGLLQREQVDLMDVSPKQVVSVAAALIPFLENDDANRALMGSNM
                     QRQAVPLIQSDAPLVGTGMEDVVARDSGAVVIARRKGVVEQIDGTR"
BASE COUNT          379 a          688 c          651 g          291 t
ORIGIN      
        1 gtctcctacg agttcgagga gccgaagtac gacgtcgagg aatgcatcca gcgcgacatg
       61 acctatgccg cgccgctgaa ggtgaagctc cgtctcatcg tgttcgagat ggaagaggaa
      121 accggcgccc ggtccgtcaa ggacatcaag gagcaggacg tctacatggg cgacatcccg
      181 ctcatgaccg acaagggcac gttcatcgtc aacggcaccg agcgggtgat cgtctcgcag
      241 atgcaccgct cgccgggcgt gttcttcgac cacgacaagg gcaagacgca cagctcgggc
      301 aagctgctgt tcgccgcccg tgtcattccc tatcgcggct cctggctcga cttcgagttc
      361 gacgccaagg acatcgtcta tgtccgcatc gaccgccgcc gcaagctgcc ggccacgacc
      421 ttcctctacg gtctgggcat ggacggcgaa gagatcctga cgaccttcta cgacgtcgtg
      481 ccgttcgaaa agcgcgagga aaagaagggc aagccgggcg gctgggccac cccctacaag
      541 cccgagcgct ggcgcggcgt gaagccggaa ttcgccctga tcgacgctga cacgggcgag
      601 gaagtcgccg ccgccggcgc caagatcagc gcccgcgccg ccaagaagct cgccgacaac
      661 ggcctcaaga ccctgctgct gtcgccggac gccctggtcg gccgctacct ggcccgcgac
      721 gaggtgaacc cggaaaccgg cgagatctac gccgaggccg gcgacgagct cgacacggcg
      781 atcatcgagg tgctcgagga gaagggcttc acgaccatcg acgtgctcga catcgaccac
      841 gtcaccgtcg gcgcctacat gcgcaacacc ctgcgcgtcg acaagaacgc cgtccgcgag
      901 gatgcgctgt tcgacatcta ccgcgtgatg cgcccgggcg agccgcccac cgtcgaagcc
      961 gccgaagcga tgttcaagtc gctgttcttc gaccaggagc gctacgacct ctcctcggtc
     1021 ggccgcgtga agatgaacat gcgcctggag caggacgtcg ccgacgacgt ccgcgtcctg
     1081 cgcaaggaag acgtgctgga agtcctcaag gtcctggttg gcctgcgcga cggccggggc
     1141 gagatcgacg acatcgacaa cctcggcaac cgtcgggtgc gctcggtcgg cgagctgctg
     1201 gaaaaccagt accgcgtcgg cctgctgcgc atggaacggg cgatcaagga gcgcatgtcc
     1261 agcgtcgata tcgacacggt catgccgcac gacctgatca acgccaagcc ggccgccgcc
     1321 gcggtccgcg agttcttcgg ctcctcgcag ctgtcgcagt tcatggacca gaccaacccg
     1381 ctgtcggaaa tcacccacaa gcgccgcctg tcggcgcttg gcccgggcgg tctgacccgc
     1441 gagcgcgccg gcttcgaggt gcgcgacgtg cacccgacgc attacggccg catctgcccg
     1501 atcgagacgc cggaaggccc gaacatcggc ctgatcaact cgctggcgac gcacgcgcgg
     1561 gtgaacaagt acggcttcat cgaaagcccg taccgccggg tgaccgacgg caagccgcag
     1621 gccgaggtcg tctacatgtc ggccatggaa gaggccaagc acgtcatcgc ccaggccaac
     1681 atccggatgg acggcggctc gatcgccgaa gatctcgttc cgggccgggt caacggcgag
     1741 ccgggcctgc tccagcgcga gcaggtcgac ctgatggacg tttcgcccaa gcaggtcgtc
     1801 tcggtcgccg cggccctgat cccgttcctc gagaacgacg acgccaaccg cgccctgatg
     1861 ggctcgaaca tgcagcgtca ggctgtgccg ctgatccagt cggacgcgcc gctggtcggc
     1921 accggcatgg aagacgtggt ggcccgcgac tccggcgccg tggtgatcgc ccgccgcaag
     1981 ggcgtggtcg agcagatcga cggcacgcg
//
LOCUS       DAORNX010000525         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41835_length_1997_cov_5.943872, whole genome shotgun sequence.
ACCESSION   DAORNX010000525 DAORNX010000000
VERSION     DAORNX010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41835_length_1997_cov_5.943872"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLO65_16170"
     CDS             complement(<1..1092)
                     /locus_tag="PLO65_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD29836.1"
                     /translation="MHFQRLRLSGFKSFVDPTEFRIEPGLTGIVGPNGCGKSNLLEAL
                     RWVMGANSAKAMRAGGMDEVIFNGAGSRPARNHAEVTLTIDNSDHVAPAQFNQHDVIE
                     VVRRIDRGEGSTYRVNGREVRARDVQLLFADASTGANSPALVRQGQISELIAAKPQNR
                     RRILEEAGGVSGLHTRRHEAELRLRAAETNLARLDDVIRELDSSLARLRREARQAERY
                     KKLSAEIRAVQGAVLFARWSEARDTLLRVEAESTSSARLVEDTARAAAIATTEAQKAE
                     EAIRPLREEETVAAAILNRLAIEKDRLEREHETAAAEVERLTAELARIDADAARESQI
                     VEDAGVALERLAAELADVEAEIAAAPSRLP"
     gene            complement(1111..1737)
                     /locus_tag="PLO65_16175"
     CDS             complement(1111..1737)
                     /locus_tag="PLO65_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD29837.1"
                     /translation="MRIDRRTILAAAAAVATLALAACGGGGGGVTAEDMSLGKPDAPV
                     TVIEYASLACSHCGRWEQDVFPAFKAKYIDTGKVHYVFREFMTSPPELAASGALLARC
                     AGKDKYFPVVQAVFHAQEEIFTTGDIRGPLLRIAETAGMSEADFDKCVGDEKALAAFN
                     ARVEKYQKEAKIEGTPTFFFNGDKYDKGEMSMAEIDAAYEKALAATKK"
     gene            complement(1842..>1997)
                     /locus_tag="PLO65_16180"
     CDS             complement(1842..>1997)
                     /locus_tag="PLO65_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29838.1"
                     /translation="TRPQALRRRPKGPLDAAAEQALADSLADQPEGPLREALIRLGRE
                     TLRGRGG"
BASE COUNT          256 a          673 c          737 g          331 t
ORIGIN      
        1 gggaaggcgc gagggcgccg cggcgatctc ggcctcgaca tcggccagtt cggcggccag
       61 ccgctccagc gccacaccgg catcctcgac gatctggctc tcgcgggccg cgtcggcgtc
      121 gatgcgcgcc agttcggcgg tcaagcgctc gacctcggcc gcggcggtct cgtgctcgcg
      181 ctccagccgg tccttctcga tggccagccg gttgaggatg gcggcggcga ccgtctcctc
      241 ctcgcgcagc ggcctgatcg cctcctcggc cttctgggcc tcggtggtgg cgatggcggc
      301 ggcgcgggcg gtgtcctcga cgaggcgggc cgagctggtc gattcggcct cgacgcgcag
      361 cagggtgtcg cgcgcctcgc tccagcgggc gaacaggact gcgccctgga ccgcgcggat
      421 ctcggccgac agcttcttgt aacgttccgc ctggcgggcc tcgcggcgca ggcgcgccag
      481 ggacgagtcc agctcacgga tgacgtcgtc gaggcgggcc aggttggtct cggccgcccg
      541 caggcgcagc tcggcctcgt ggcggcgggt gtgcaggccg ctgaccccgc cggcttcctc
      601 gaggatgcgg cggcgattct gcggcttggc cgcgatcagt tcgctgatct ggccctggcg
      661 gaccagggcc ggggagttgg ccccggtcga ggcgtcggcg aacagcagct gcacgtcgcg
      721 ggcccggacc tcgcggccgt tgacccggta ggtcgagccc tcgccgcggt cgatgcggcg
      781 aaccacctcg atgacgtcat gctggttgaa ctgggccggg gcgacatggt cgctgttgtc
      841 gatggtcagg gtgacttcgg cgtggttacg ggccggccgc gagccggcgc cgttgaagat
      901 cacctcgtcc atgccgccgg cccgcatggc cttggcggaa ttggccccca tcacccagcg
      961 cagggcttcc agcaggttcg acttgccgca gccgttcggc ccgacgatgc ccgtgagtcc
     1021 gggctcgatc cggaactcgg tcggatcgac gaaggacttg aacccggaaa ggcgcagccg
     1081 ctggaagtgc acgggtccgg tggaccttcc ctatttcttc gtcgccgcca gcgccttttc
     1141 gtaggcggcg tcgatctcgg ccatggacat ctcgcccttg tcgtacttgt cgccgttgaa
     1201 gaagaaggtc ggggtgccct cgatcttcgc ctccttctgg tacttctcga cccgggcgtt
     1261 gaaggcggcc agggcctttt cgtctcccac gcacttgtcg aagtcggcct cgctcatgcc
     1321 agcggtctcg gcgatgcgca gcagggggcc gcggatgtcg ccggtggtga agatctcttc
     1381 ctgggcgtgg aacacggcct ggacgacggg gaagtacttg tccttgccgg cgcagcgggc
     1441 cagcagggcg ccggatgcgg ccagctccgg cggcgaggtc atgaactcgc ggaacacgta
     1501 gtggaccttg ccggtgtcga tgtacttggc cttgaaggcg gggaagacgt cctgctccca
     1561 acgaccgcaa tggctgcagg ccagcgaggc gtattcaatc accgtgaccg gggcgtcggg
     1621 cttgcccagg ctcatgtctt cggcggtgac tccgccgccg ccgccgccac aggcggccag
     1681 ggcgagggtg gcgacggccg cggcggcggc caggatggtg cggcgatcaa tgcgcatgtc
     1741 gggatgtccc ccagaagagc gaacggccat tagagcccga ttccacaggc tgcaagaagg
     1801 cggcgtttat ggggcgcaac agcccgccgc gggaccggtt gtcaaccgcc gcggccgcgc
     1861 agggtctccc gccccagccg gatcagggcc tcgcgcagcg gtccttcggg ctggtcggcg
     1921 aggctgtcgg ccagggcctg ttcggcggcg gcgtcgagcg gacccttcgg ccggcgacgc
     1981 agggcctgtg gccgcgt
//
LOCUS       DAORNX010000526         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41967_length_1991_cov_7.356921, whole genome shotgun sequence.
ACCESSION   DAORNX010000526 DAORNX010000000
VERSION     DAORNX010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41967_length_1991_cov_7.356921"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PLO65_16185"
     CDS             <1..1519
                     /locus_tag="PLO65_16185"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRD29839.1"
                     /translation="SAAEAESARRRDLLVFDDALRGAGGARVSLAATEVFDGASVSVL
                     HVTFGGPDAYDLLIDPATGALKAERRLEDGRTILVRYDDWRTVEGVRMPFVETLEEAG
                     DPIKRRLTFTAMDIDPVVSPTVWSRPADRAVASFAPGARATAPLPFEFFLGSRIYIPA
                     TVAGRTTHVLLDSGAETTVLDSRFAEAAGIKVSGVVTAVGTGGRQEAQLASGVTIRIG
                     EVALRDLTVALVDLSAIEQMIGRPIPVILGKEVLNALTVDLDFQGKTIAFEDPARFTP
                     PAGAVEVPVTSANGIRSVPVSIEGGPPIQVDFDLGNGSAFLAYSAWWKPAGTLSDGRP
                     VSQTLSGAIGGLKTRSIASLRAVTFAGVTFRNVPTVFFDDDGQSAESNRTFGNVGMPI
                     LSRFRLTTDYSRDRLFLTPLPDAATRPFAKDRAGVLARPARDGKAEILLVAPGSPAEA
                     AGLKTGETITAVNGLALTGNNAQAIGAIRALPAGSSVSFTLSTGEIRTVVLKDYF"
     gene            complement(1524..>1991)
                     /locus_tag="PLO65_16190"
     CDS             complement(1524..>1991)
                     /locus_tag="PLO65_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRD29840.1"
                     /translation="RALADRFESWDEFVQTTRAAAAGAPGEAFLALAAIDGVGLVAAQ
                     SVADFFREDHNRALVEALVAELDAIQPLAKAKTDTAIAGKTVVFTGALEKMTRDEAKA
                     QAEALGARTAGSVSKKTDLVVAGPGAGSKLKDAEKHGVKVLTEDEWLALIGAG"
BASE COUNT          277 a          739 c          675 g          300 t
ORIGIN      
        1 gtcggcggcc gaggccgagt cagcgcggcg gcgcgacctg ctggtgttcg acgacgccct
       61 gcgcggcgcc ggcggcgccc gcgtcagcct ggccgccacg gaggtcttcg acggcgcgtc
      121 ggtgtcggtg ctgcatgtca ccttcggcgg gccggacgcc tatgacctgc tgatcgatcc
      181 cgcgaccggc gccctgaagg ccgaacggcg gctcgaggat ggtcggacga tcctggtccg
      241 gtacgacgac tggcgcacgg tcgagggcgt gcgcatgccg ttcgtcgaaa cgctggagga
      301 agccggcgat ccgatcaaac gccgcctgac cttcacggcg atggacatcg atccggtcgt
      361 ctcgccgacc gtctggtccc gccccgcgga ccgggccgtg gccagcttcg cgccaggtgc
      421 ccgggccacc gcgcccctgc ccttcgagtt cttcctcggc tcgcgcatct acatccccgc
      481 caccgtcgcc ggcaggacga cgcatgtgct gctcgacagc ggggcggaga ccacggtgct
      541 cgacagccgc ttcgccgagg ccgccggcat caaggtgtcg ggcgtcgtca ccgccgtcgg
      601 cacgggcggg cgacaggagg cccaactggc ctccggcgta accatccgca tcggtgaagt
      661 cgcactcagg gatctgacag tcgccctcgt cgacctgtcg gccatcgaac agatgatcgg
      721 ccgaccgatc ccggtgattc tcggcaagga ggtgctcaac gccctgaccg tcgacctcga
      781 cttccagggc aagaccatcg ccttcgagga tcccgcccgg ttcacgccgc cggccggcgc
      841 ggtggaagtg ccggtgacct cggccaacgg catccgctcc gtgcccgtct cgatcgaggg
      901 cggcccgccg atccaggtcg acttcgacct cggcaacggc tcggccttcc tcgcctattc
      961 ggcctggtgg aagccggccg ggaccctttc cgacgggcgg ccggtgtcgc agaccctctc
     1021 gggcgccatc ggcggcctga agacgcgcag catcgccagt ctgcgggcgg tgaccttcgc
     1081 cggggtgacc ttccgcaacg tgcccaccgt gttcttcgac gacgacggcc agagcgccga
     1141 aagcaaccgc accttcggca acgtcggcat gcccatcctc agccgcttca ggctgacgac
     1201 cgactattcg cgcgaccggc tgttcctgac tccgctgccg gacgcggcga cccggccgtt
     1261 cgccaaggac agggcaggcg tgctggcccg gccggcccgc gatggaaagg cggagatcct
     1321 gctggtggcg cccgggtctc cggcggaagc cgcggggctg aagaccggcg aaaccatcac
     1381 cgcggtcaac ggactggccc tgaccgggaa caacgcccag gcgatcgggg ccatccgcgc
     1441 cctgccggcc ggctcgagcg tgagcttcac cctctccacc ggcgagatcc gcacggtggt
     1501 gctgaaggac tatttctaga ccgtcagccg gccccgatca gggccagcca ttcgtcctcg
     1561 gtcagcacct tgacgccgtg cttctcggca tccttgagct ttgagccggc gccggggcca
     1621 gcgacgacga ggtccgtctt cttcgacacc gagccggcgg tcctggcgcc cagcgcctcg
     1681 gcctgggcct tggcctcgtc gcgggtcatc ttctccagcg cgccggtgaa gaccaccgtc
     1741 ttgccggcga tggccgtgtc ggtcttggct ttcgccaggg gctggatcgc gtccagctcg
     1801 gccaccaggg cctcgaccag cgcgcgattg tggtcctcgc ggaagaagtc ggccaccgac
     1861 tgggccgcga ccagcccgac cccgtcgatc gccgccaggg ccaggaaggc ctcgccgggc
     1921 gcgccggcgg ccgcggccct cgtggtctga acgaactcgt cccagctctc gaaccggtcg
     1981 gccagggccc g
//
LOCUS       DAORNX010000527         1990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_41997_length_1990_cov_6.800517, whole genome shotgun sequence.
ACCESSION   DAORNX010000527 DAORNX010000000
VERSION     DAORNX010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41997_length_1990_cov_6.800517"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLO65_16195"
     CDS             complement(<1..220)
                     /locus_tag="PLO65_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HRD29841.1"
                     /translation="MDLAAFMRLLAAGWTLIRADALIPRELDAVLPPGVRTLAGVLRL
                     FSGRQARQGRPGERLARSLERLGPVAIKL"
     assembly_gap    290..382
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<383..985)
                     /locus_tag="PLO65_16200"
     CDS             complement(<383..985)
                     /locus_tag="PLO65_16200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRD29842.1"
                     /translation="MSESTATFGYRDVDPTEKPRLVRGVFDRVASRYDLMNDLMSAGV
                     HRLWKDATAARLNPQPGEVILDVAGGTGDMARRYATMARAAQERRGGEDATIHIIDYN
                     AEMIAAGREKGAPPEVCWVVGDAQRLPLPDASADAYSIAFGIRNVTDVAAALREARRV
                     LRPGGRFVCLEFSKPTGMALRAAYDAWSFKAIPQIGEWVAG"
     gene            1212..>1990
                     /gene="mutM"
                     /locus_tag="PLO65_16205"
     CDS             1212..>1990
                     /gene="mutM"
                     /locus_tag="PLO65_16205"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRD29843.1"
                     /translation="MPELPEVETVRRGLAPVLEGARLTRVEQRRPDLRFPFPEGFVQR
                     LTGATIARLDRRAKYLLGHLDRGDVLVMHLGMTGRFEIEAAEGAVRPGEFVYAAPPDP
                     KHAHVVFETDRGGRVTYSDPRRFGFMGLVPADDLETHPWFAGMGPEPLGPEFNAGLLE
                     RAFAGRKQNAKVLLLDQRIVAGLGNIYVCEALHRSGISPTKPAGRIPKKALPGLVAVI
                     RAVLDEAIEAGGSTLRDFSAADGALGYFQHRFQAYGREGQA"
BASE COUNT          260 a          683 c          676 g          278 t
ORIGIN      
        1 ccagcttgat cgccacgggc cccagccgct cgagcgaccg cgccagccgc tcgcccggcc
       61 ggccctgacg cgcctggcgg ccggaaaaca gtctcaggac accggccagg gtccgcacgc
      121 cgggcggcag gaccgcgtcc agctcccgcg ggatcagggc gtcggcgcgg atcagcgtcc
      181 agccggcggc cagcaatcgc atgaatgcgg ccagatccat ggcgctagag aacccgcccc
      241 gcatgcaggg ccgccacgcc accggtgaag ttgctgtagg tgacccgggn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nngccggcga cccattcgcc gatctgcgga atggccttga
      421 acgaccaggc gtcataggcc gcgcgcaggg ccatgccggt gggcttgctg aactcgagac
      481 agacgaaccg cccgccgggc ctgagcaccc gccgcgcctc gcgcagggcc gcggcgacgt
      541 cggtcacgtt gcggatgccg aaggcgatcg aataggcgtc ggcgctggcg tcgggcagcg
      601 gcagccgctg ggcgtcgccg accacccagc agacctcggg cggcgcgccc ttctcccgcc
      661 ccgcggcgat catctcggcg ttgtagtcga tgatgtggat ggtcgcgtcc tcgccaccgc
      721 gccgttcctg ggccgctctg gccattgtcg cgtagcgccg ggccatgtcg ccggtgccgc
      781 cggccacatc gaggatcacc tcgccgggct gggggttgag gcgggccgcc gtggcgtcct
      841 tccacaggcg atggacgccc gcgctcatca ggtcgttcat caggtcgtag cggctggcga
      901 cccggtcgaa tacgccgcgc accaggcggg gcttctcggt cggatcgacg tcgcggtagc
      961 cgaaggtggc ggtgctctcg ctcatgcggt tgctcataga cccgcgcggg cgggtcggct
     1021 agccggcgag gctcctttgc aatcctccca tggcggagat cgacctgatg tcttgatgag
     1081 cattcttgct ccggcgggac cttcgccaag gccttcaggg cgcatcagca tccgacctgg
     1141 ctgacggtgg gcgtcagcaa ccggtcagcg acgcatggac ccggccgccg cggtccgcta
     1201 gccaggaccc catgcccgag cttcccgaag tcgagaccgt ccgccgcggc ctggcccccg
     1261 tactggaggg cgcgcggctg acgcgggtcg agcagcggcg gccggacctg cggttcccgt
     1321 ttccggaggg cttcgtccag cggctgaccg gggcgacgat cgcgcggctg gaccgccggg
     1381 cgaaatacct gctcggccat ctcgaccgcg gcgacgtcct ggtcatgcac ctgggtatga
     1441 ccggccggtt cgagatcgag gcggcggagg gcgcggtgcg acccggcgag ttcgtctacg
     1501 ccgccccgcc cgacccgaaa cacgcccatg tcgtgttcga gaccgatcgc ggcgggcggg
     1561 tgacctattc cgacccgcga cggttcggct tcatggggct tgtgccggcc gacgacctgg
     1621 agacccaccc gtggttcgcc ggcatgggcc ccgagccgct cggtccggag ttcaacgccg
     1681 gcctgctgga gcgcgccttc gccgggcgca agcagaacgc caaggtcctg ctgctcgacc
     1741 agcggatcgt cgcgggcctg ggtaacatct atgtctgcga ggccctgcac cggtccggca
     1801 tctccccgac gaagccggcc ggcaggatcc cgaagaaggc cctgcccggc ctggtcgcgg
     1861 tgatccgcgc cgtgctggac gaggccatcg aggccggcgg ttcgaccctg cgcgactttt
     1921 cggcggccga cggcgcgctg ggctatttcc agcaccgttt ccaggcctac ggccgcgagg
     1981 gccaggcctg
//
LOCUS       DAORNX010000528         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_42513_length_1971_cov_5.536534, whole genome shotgun sequence.
ACCESSION   DAORNX010000528 DAORNX010000000
VERSION     DAORNX010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42513_length_1971_cov_5.536534"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PLO65_16210"
     CDS             complement(<1..994)
                     /locus_tag="PLO65_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD29844.1"
                     /translation="MFRPAKALVLALALSSSTLVGGAWAQAAPAAVVAPAATEVPPIG
                     FKSRVLANGMKLFYSLDRATPNVTVQVWYGVGAKDDPAGRSGFAHLFEHLMFKATRNL
                     PAESFDRMTEDVGGFNNASTWSDFTNYYEVVPASHLQRLLWAESERLGSLVVDEASFK
                     SERDVVKEELRLRVLADPYGRFFRIAIPSASYTTHPYKRPGIGSIEDLDAATIDDVLA
                     FHATYYRPDNAALIVVGNFDEAELNAWVDRYFGSLKTPATPLPRVTVREPARTASRTV
                     TSYAPNVPLPAVAITWQGPAAADPDAAALRVLDAILSGGKSSRLYNSLVYDQQIA"
     gene            1125..1832
                     /locus_tag="PLO65_16215"
     CDS             1125..1832
                     /locus_tag="PLO65_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29845.1"
                     /translation="MMSKVIAAAAAAILLAGGGVALAQDSDVSEIVVTGSRMMDYEPT
                     ETPAIVLRKRADNVLTSIEVTCDTRDPAQRKAELKTTLQALIRGAAAAGIELAVESDD
                     IVRRFNPDDLDAIIRAASKADTSTATLTLKTRVQPTDSFESVTGRVAAFVKKTPKTGR
                     TEVLMSNDWDLTLVGPGQYRGDIIALVAADANRTAAAFGPAYSVSIDGLTLPVSWYQS
                     GPLELALYIPYRQAISK"
BASE COUNT          318 a          655 c          725 g          273 t
ORIGIN      
        1 gggcgatctg ctgatcatag accaggctgt tgtagagccg cgaggacttg ccgcccgaga
       61 ggatggcgtc gagcacccgc agcgccgcgg cgtcgggatc ggcggcggcc gggccctgcc
      121 aggtgatggc caccgccggc aggggcacgt tcggggcgta gctggtgacg gtgcggctgg
      181 ccgtgcgggc cggctcgcgc acggtcaccc gcggcagcgg cgtggccggc gtcttgagcg
      241 atccgaaata gcggtcgacc caggcgttca gctcggcctc gtcgaagttg ccgaccacga
      301 tcagcgccgc gttgtcgggc cggtagtagg tggcgtggaa ggccagcacg tcatcgatcg
      361 tcgccgcgtc gagatcctcg atcgaaccga tgccgggccg cttgtagggg tgggtggtgt
      421 agctggcgct cgggatggcg atgcggaaga agcggccata ggggtcggcc agcacccgca
      481 ggcgcagctc ctccttcacc acgtcgcgct ccgacttgaa gctggcctcg tcgaccacca
      541 gcgagccgag gcgctcggac tcggcccaca gcagccgctg tagatggctg gccggcacca
      601 cctcgtaata gttggtgaag tccgaccagg tggaggcgtt gttgaagccg cccacgtcct
      661 cggtcatgcg gtcgaacgac tcggcgggca ggttgcgcgt ggccttgaac atcagatgtt
      721 cgaacaggtg ggcgaagccc gagcgaccgg cgggatcgtc cttggcgccg acgccgtacc
      781 agacctgcac ggtgacgttc ggcgtcgcgc ggtcgagcga gtagaacagc ttcatgccgt
      841 tggccagaac ccggctcttg aagccgatcg gcgggacctc ggtcgcggcg ggcgcgacga
      901 cggcggccgg ggcggcctgg gcccaggcgc ctccgaccag ggtcgaggac gagagggcga
      961 gggcgagaac aagggccttg gccggacgga acatgcggaa ctccgggtga acgtcgggcg
     1021 cgaccctagc accggccgtc gggctggaaa ccttacgaac ctgtcatgtg gctggacgga
     1081 tcgcggaggt ggggccaagc tgacggtgaa acggggaggt cgtcatgatg tcgaaggtaa
     1141 tcgcggccgc cgcggcggcc attctgctgg cgggcggcgg cgtcgccctg gcccaggaca
     1201 gcgacgtgtc ggagatcgtc gtcaccggct cgcggatgat ggactacgag ccgaccgaga
     1261 cgccggcgat cgtgctccgg aagcgggccg acaacgtcct gacgagcatc gaggtgacct
     1321 gcgacacccg tgaccccgcc cagcgcaagg ccgagctgaa gaccaccctc caggcgctga
     1381 tcaggggcgc cgccgcggcg gggatcgagc tggcggtcga gagcgacgac atcgtccgcc
     1441 ggttcaaccc cgacgacctc gacgccatca tccgcgcggc gagcaaggcc gacaccagca
     1501 ccgcgaccct gacgctcaag accagggtcc agccgaccga cagcttcgag tccgtcaccg
     1561 gccgggtggc cgccttcgtc aagaagacgc cgaagacggg gcgaaccgag gtgctgatga
     1621 gcaatgactg ggacctgacg ctggtcgggc ccggccaata tcgcggcgac atcatcgccc
     1681 tggtcgccgc cgacgccaac cggaccgcgg ccgccttcgg tccggcctac agcgtgtcga
     1741 tcgacggcct gacgctgccg gtgtcctggt accagtccgg cccgctcgaa ctggcgctgt
     1801 acatcccgta ccggcaggcg atttcgaagt aggcggacag ccagtccgcg ccccgggcgg
     1861 cgtccgtcag ccgccggcct ggcgcgctgt ggcttccgcc tcgcggcgca gggcgcgcag
     1921 ggcgtcggcg acatccatgc gcatcgccgc cctggcgatc atcgccggac c
//
LOCUS       DAORNX010000529         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_42959_length_1954_cov_6.549763, whole genome shotgun sequence.
ACCESSION   DAORNX010000529 DAORNX010000000
VERSION     DAORNX010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42959_length_1954_cov_6.549763"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PLO65_16220"
     CDS             complement(<1..1214)
                     /locus_tag="PLO65_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD29846.1"
                     /translation="MDDAASPNPDTEETARLQALRALHALDSGSDSRFDRIARTAALV
                     LRAPRAAIILVDADRLWHKARVGIPSGQYPRAGTLADLMIRHPGVIFSLDVQSDPRFD
                     PVRDSLHQVDVRFYAAAPLIAPGGAVIGLLAVGDTEAHAPHTEAERLALEDLAALAME
                     MLVHDAENIDAERRSSLDQRRVELALEAAGLGEFEWDIARDRMFISDRMKSLIGVNAR
                     STRGEGGDVSFRFVHPDDAQRLRETVDRGLRTEGRYSAEYRMIRPDDGRLRWMQGAGV
                     VVSDRDGSPRRVIGIVRDITDRKTEEEHRETLLAELDHRVKNVLAAVQSLAAQSARRT
                     TSMEGFLSAFAGRLKAMASAHELLTATRWRGASLAHIAAAELGGLAPGQARWEGPDLV
                     LTPRAANAVSLAL"
     gene            1382..1732
                     /locus_tag="PLO65_16225"
     CDS             1382..1732
                     /locus_tag="PLO65_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29847.1"
                     /translation="MPTNATTPAAAGETDQAKADLKSAARHAERSFIEATDQARERLT
                     EAAHRTEAAVREGMETLRAQSRAYVDTAGQQFDEAQRYVVEKVKERPVTATLAGLGVG
                     VLIGLLLSSRSDRR"
     gene            1750..>1954
                     /locus_tag="PLO65_16230"
     CDS             1750..>1954
                     /locus_tag="PLO65_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29848.1"
                     /translation="MAVAAGAAIAAAAAAGVFSAFFALFAFLQPFVGTAGAAAIIAGC
                     SAVVIGGAGFLAARKAEGERKAEA"
BASE COUNT          273 a          698 c          712 g          271 t
ORIGIN      
        1 agcgcgaggg aaacggcgtt ggccgcccgc ggcgtcagca ccagatccgg accctcccag
       61 cgcgcctgac ccggcgccag gccgccgagc tcggcggcgg cgatatgggc caggctggcg
      121 ccgcgccagc gggtcgcggt caacagctcg tgcgccgagg ccatcgcctt gagccgtccg
      181 gcgaaggccg agaggaagcc ctccatggag gtggtccggc gggccgactg cgccgccaga
      241 gactggaccg ccgccagcac gttcttcacc cggtggtcga gctcggccag aagggtctcg
      301 cggtgctcct cctcggtctt tcggtcggtg atgtcgcgca cgatgccgat gacgcgccgg
      361 ggcgagccgt cccggtcgga gaccaccacg cccgcgccct gcatccagcg cagccggcca
      421 tcgtccggcc ggatcatgcg gtactcggcc gaatagcgac cttccgtgcg cagcccgcgg
      481 tcgaccgtct cgcggagccg ctgggcgtcg tcggggtgga cgaaccggaa ggagacgtcg
      541 cccccctcgc cgcgcgtcga acgggcgttg acgccgatca ggctcttcat ccggtcgctg
      601 atgaacatcc ggtcgcgggc gatgtcccat tcgaactcgc cgaggccggc ggcttccagc
      661 gccagctcga cgcggcgctg gtcgaggctg ctgcgccgtt cggcgtcgat gttctcggcg
      721 tcgtgaacca gcatctccat ggccagggcg gccaggtcct ccagcgccag gcgttcggcc
      781 tcggtatgcg gcgcatgggc ctcggtgtcg ccgaccgcca gcaggccgat caccgccccg
      841 ccgggggcga tcagcggcgc ggccgcatag aaccgcacat cgacctggtg aaggctgtcg
      901 cggaccggat cgaaccgcgg gtcgctctgc acgtcgagac tgaagatcac gcccggatgg
      961 cggatcatca ggtcggcgag ggtgcccgcg cgcggatact ggccggaggg gatgccgacc
     1021 cgcgccttgt gccagaggcg gtcggcgtcg accaggatga tggccgcccg gggcgcccgc
     1081 aggaccaggg cggccgtgcg ggcgatccgg tcgaaccggc tgtccgaacc gctgtcgagc
     1141 gcatgcaggg cgcgcaacgc ctgcaggcgg gccgtctcct cggtgtccgg gttcggcgat
     1201 gccgcatcat ccatgggctc accataacgg gtaagtcctg gtcgcgcgac aggtgagaat
     1261 ccgtagcgct caagggcttg tcgcggtagg atggaacttg atctcgcggg atggcgttcc
     1321 cggttggtcc cccgcctgga gggccgccgg atgcggcgcg cccgcgaata cggagcctgt
     1381 catgcccacc aacgccacga cccccgccgc cgccggcgaa accgatcagg ccaaggccga
     1441 cctgaagagc gccgcccgcc acgccgagcg cagtttcatc gaggcgaccg atcaggcccg
     1501 cgagcgcctg accgaggcgg cgcaccggac ggaggccgcc gttcgcgagg gtatggagac
     1561 cctgcgcgcc cagagccgcg cctatgtcga caccgccggc cagcagttcg acgaagccca
     1621 gcgctacgtc gtcgagaagg tcaaggagcg gccggtcacg gccaccctgg ccggcctcgg
     1681 cgtgggcgtg ctgatcggac tcctgctctc cagccgaagc gatcgccgct gatgatggga
     1741 cggacgctga tggctgtggc ggccggcgcc gctatcgccg ccgccgcagc cgccggcgtc
     1801 ttctcggcct ttttcgcgct gttcgccttc cttcagccct tcgtgggcac ggcgggggcg
     1861 gcggcgatca tcgccggctg ctcggccgtg gtcatcggcg gggccggctt cctggccgcc
     1921 cgcaaggcgg agggcgagcg caaggccgag gccg
//
LOCUS       DAORNX010000530         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43055_length_1950_cov_5.526649, whole genome shotgun sequence.
ACCESSION   DAORNX010000530 DAORNX010000000
VERSION     DAORNX010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43055_length_1950_cov_5.526649"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLO65_16235"
     CDS             <1..658
                     /locus_tag="PLO65_16235"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRD29849.1"
                     /translation="GGSYLLDKVCHDFDIFGRIAGSRAARVASFGGRRIFTPERAAAP
                     RVYSTGEAAFALRDAGWSGANDSFQSDMDVTDHQTAMVEYENRVRLTFHANSQVSLAE
                     RRWYIAGTEGTLIADLVRNQLMARGIMDRSKPERIDYGGRTADSHNGADQAMARDLKA
                     TLEGRAAFPVSPWDSMEAGLTVMAIDQAMAEGAMIDCGPMWARYDAARGVSSAGPSNS
                     "
     gene            complement(610..1374)
                     /locus_tag="PLO65_16240"
     CDS             complement(610..1374)
                     /locus_tag="PLO65_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRD29850.1"
                     /translation="MTAILAIHAHPDDIETLGAGALALLAAGGHAITLATLTAGEGGS
                     TDTPPEETARIRRGEAAAAAALIGADYHCAGLPDLGVFSDDAARRTVTGLIRRARPDI
                     VITSAPADYHPDHEATSQLVRDACFAASVPNYQAGDAPALDAIPHLYFMDPIGGRDRE
                     GRPVTADFGADIEAVFATKQAMLAAHESQIRWVARQHGVPDQVASMEKWARRRGRDFG
                     VAMAEGFRQYRHHPWPATPRLQELLGPALLTPLAAS"
     gene            complement(1392..>1950)
                     /locus_tag="PLO65_16245"
     CDS             complement(1392..>1950)
                     /locus_tag="PLO65_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29851.1"
                     /translation="GGYYSPPTATGAIQGLDVQGVHRTRQVAYEATRSKMRRVVIQAF
                     CLDDRDVPHPASQVFPERDVDDQYEGELYRCIAGARMQVTIAEFSGQVSFSGGQTMIC
                     AKGEALYRSGGSSPDARFLGCRPQKPARDCNERSLLRRFGAGIKVLTIVTMEKYTAYR
                     EEVVTESSSASYSMSLDGGVGGVAY"
BASE COUNT          278 a          677 c          711 g          284 t
ORIGIN      
        1 gggcggctcc tacctgctcg acaaggtgtg ccacgacttc gatatcttcg ggcgcatcgc
       61 cgggtcccgg gcggcccggg tggccagctt cggcggacgg cgcatcttca cccccgaacg
      121 ggccgccgcg ccgcgggtct acagcaccgg cgaggcggcc ttcgccctgc gcgacgcggg
      181 ctggtcgggc gccaacgaca gcttccagtc cgacatggac gtcaccgacc accagaccgc
      241 gatggtcgag tacgagaacc gggtgcggct gacctttcac gccaacagcc aggtctcgct
      301 ggccgagcgt cgctggtaca tcgccgggac ggaagggacg ctgatcgccg acctcgtgcg
      361 caaccagctg atggcgcggg gcatcatgga tcgctccaag cccgagcgga tcgactatgg
      421 cggccgcacg gccgacagtc acaacggcgc cgaccaggcc atggcccgcg atctgaaagc
      481 cacgctggaa ggccgggccg cctttccggt ctcgccgtgg gactcgatgg aggccgggct
      541 gacggtgatg gcgatcgacc aggccatggc cgagggcgcg atgatcgact gcggaccgat
      601 gtgggcccgc tacgacgcgg ccaggggcgt cagcagcgcc ggcccgagca actcctgaag
      661 cctgggcgtc gccggccagg gatggtggcg gtactggcgg aagccctcag ccatcgccac
      721 gccgaagtcg cgcccgcgcc gccgcgccca cttttccatg gaagcgacct ggtcggggac
      781 gccgtgctgg cgggcgaccc agcggatctg gctctcgtgc gccgccagca tcgcctgttt
      841 ggtggcgaag acggcctcga tgtcggcccc gaaatccgcc gtcacgggcc ggccctcgcg
      901 gtcgcggccg ccgatcgggt ccatgaagta gaggtgcgga atggcgtcca gcgccggggc
      961 gtccccggcc tggtagttgg gcaccgaggc cgcgaaacag gcgtcgcgga ccagctggct
     1021 cgtcgcctca tggtcgggat gatagtcggc cggggcgctg gtgatgacga tgtccggacg
     1081 cgcccggcgg atcagaccgg tcaccgtccg gcgcgcggcg tcatcgctga acacccccag
     1141 gtccggaagg ccggcgcagt ggtagtccgc cccgatcagc gccgccgcgg ccgcggcctc
     1201 gccccggcgg atgcgcgccg tctcttcggg cggggtgtcg gtcgatccgc cctcgccggc
     1261 cgttagcgtc gccagggtga tggcgtgacc gccggcggcc agcagcgcca gcgcgcccgc
     1321 gccgagcgtc tcgatgtcgt cgggatgggc atggatggcc aggatggcgg tcatggttcg
     1381 atcgacgacc gtcagtaggc gactccgccg actccgccgt ccaggctcat gctgtagctg
     1441 gcggacgagg attctgtgac cacctcctcg cgataggccg tgtacttctc catcgtcacg
     1501 atggtcagca ccttaatgcc cgcgccgaag cggcgcagca ggctgcgctc gttgcagtcg
     1561 cgggccggct tctgcggccg acagccgagg aagcgggcgt ccggcgagga tccgcccgac
     1621 cggtacagcg cctcgccctt ggcgcagatc atcgtctggc cgccggagaa ggagacctgg
     1681 cccgagaatt cggcgatcgt cacctgcatg cgggcgccgg cgatgcagcg gtagagctcg
     1741 ccttcgtact ggtcgtcgac gtcgcgctcc gggaagacct ggctggccgg gtggggcaca
     1801 tcgcggtcgt cgaggcagaa ggcctggatg accacccggc gcatcttgct gcgggtcgcc
     1861 tcgtaggcca cctgccgcgt gcggtggacg ccctggacgt cgagtccctg gatcgcgccc
     1921 gtggccgtcg gcggcgaata gtagccgccg
//
LOCUS       DAORNX010000531         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43128_length_1948_cov_5.371368, whole genome shotgun sequence.
ACCESSION   DAORNX010000531 DAORNX010000000
VERSION     DAORNX010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43128_length_1948_cov_5.371368"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLO65_16250"
     CDS             complement(<1..252)
                     /locus_tag="PLO65_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD29852.1"
                     /translation="MSQSPFQDRVAVVTGGESGIGAACALALGLAGARVAITFFADQA
                     AADRVCGAIGGPDRAIAVRTDVADEAAVEALFRGVEDAFG"
     gene            complement(351..>1948)
                     /locus_tag="PLO65_16255"
     CDS             complement(351..>1948)
                     /locus_tag="PLO65_16255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD29853.1"
                     /translation="LPGGISDFSLSPDGRRAVVVAETGGPAGNAAGTTPPIVIDRYQF
                     KEDGRDYLDARRQQLFIVDLASGKATALTHGDFDHWLPAWSPDGRTIAFVSKRSGADP
                     DRNIDFDVFVVAADGGEPRRLGDFAGSDNDPTWESRPAWSPDSTRLVWLRSGEDKWIY
                     YAPWQMVVAEVATGAITTPAWIDRCFYHPRWSADGKRLLALVEQDRDTWLASIDPATG
                     EIDYLTAGARFAYDFAVGPDGRLAVLDGTSDAPYALRTVEPTPRVLTGHNAWLAHRAL
                     ATTRDVSFMSDGVEVHGILTLPPGFDPARRYPTIVRLHGGPVYQFSHEFMFDWQLYAA
                     RGYVVLGINPRGSSGRGFDFARAIYADWGNVDVRDVKAGVDWLLASGLADPDRLGVGG
                     WSYGGILTNYVVASDPRFRAAISGAGMANFLGGYGADMYVREYELELGQPWKDTDTWL
                     KLSYPFLHADRISTPVLFQCARSDVNVPCIGAEQMYQALRSRGLDTRLVIYPDQTHGL
                     TTPSYRADRLRRNLDWYDRHLAP"
BASE COUNT          283 a          681 c          714 g          270 t
ORIGIN      
        1 gccgaaggcg tcctccacgc cccggaacag cgcctccaca gccgcctcgt ccgcgacgtc
       61 ggtgcgcacc gcgatcgcac ggtccggtcc gccgatcgcg ccgcagacgc ggtcggcggc
      121 cgcctggtcc gcgaagaagg tgatcgccac ccgggcgccg gccaggccga gggcgagggc
      181 gcaggccgcg cctattccgg actcaccgcc cgtgacgacg gcgacgcggt cctgaaacgg
      241 cgattggctc atgacctgat ccggacggcc ggcgaggtcc ggccctgtct gctaaccgcg
      301 ccgaaggccg atggtttccc gctcgcgcga aaatcggccc gcccgacggg tcagggcgcc
      361 agatggcggt cgtaccagtc gagattccgc cgcagccggt cggcgcggta gctgggcgtg
      421 gtcaggccgt gggtctggtc cggatagatc accagccggg tgtcgaggcc ccgcgagcgc
      481 agcgcctggt acatctgttc cgctccgata cagggcacat tgacgtcgga ccgggcgcac
      541 tggaacagca cgggcgtgct gatccggtcg gcgtgcagga acgggtagct gagcttgagc
      601 caggtgtcgg tatccttcca gggctggccc agctccagct cgtactcgcg gacgtacatg
      661 tccgcgccat agccgccgag gaagttggcc atcccggcgc cgctgatcgc ggcccggaag
      721 cgcgggtcgc tggccacgac gtagttggtc aggatgccac cgtagctcca gccgccgacg
      781 ccgagccggt ccgggtcggc caggcccgag gccagcagcc agtcgacccc ggccttgacg
      841 tcgcgcacgt cgacattgcc ccagtcggcg tagatcgcgc gggcgaaatc gaagccccgg
      901 cccgatgagc cgcgcgggtt gatccccagc acgacatagc cgcgcgccgc gtacagctgc
      961 cagtcgaaca tgaactcgtg gctgaactgg tagaccggcc cgccgtgcag ccggacgatg
     1021 gtcgggtagc gcctggccgg atcaaagccc ggcggcaggg tcaggatgcc gtggacctcg
     1081 accccgtcgc tcatgaagct gacgtcgcgg gtcgtggcca gcgcgcgatg ggccagccag
     1141 gcgttgtggc cggtcaggac gcggggggtc ggctcgaccg tgcgcagggc gtagggcgcg
     1201 tccgacgtcc cgtcgagcac ggccagccgg ccgtccggcc cgaccgcgaa atcgtaggcg
     1261 aaccgcgcgc ccgcggtcag gtagtcgatt tcgcccgtcg ccgggtcgat gctggccagc
     1321 caggtgtcgc ggtcctgctc gaccagcgcc agcaggcgct tgccgtcggc cgaccagcgc
     1381 gggtgataga agcagcggtc gatccaggcg ggcgtggtga tcgcgccggt cgcgacctcg
     1441 gccacgacca tctgccaggg tgcgtagtag atccatttgt cctcgcccga ccgcagccag
     1501 acgagccttg tcgagtcggg cgaccaggcc gggcgcgact cccaggtcgg gtcgttgtcg
     1561 gagcccgcga agtcgcccag ccgccgcggc tcgccgccgt cggccgcaac cacgaacacg
     1621 tcgaagtcga tgttgcggtc cgggtcggcc ccgctgcgct tggacacgaa cgcgatggtc
     1681 cggccgtcgg gcgaccaggc cggcagccag tggtcgaagt cgccgtgggt cagggccgtg
     1741 gccttgccgc tcgccaggtc gacgatgaac agctgctggc ggcgcgcgtc gagatagtcg
     1801 cggccatcct ccttgaactg gtagcggtcg atgacgatcg gcggtgtcgt tccggccgcg
     1861 ttgccggcgg gaccgccggt ctcggccacg accaccgcgc ggcggccgtc gggggacagg
     1921 gagaagtcgc tgatcccgcc gggcaggc
//
LOCUS       DAORNX010000532         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43326_length_1940_cov_6.423342, whole genome shotgun sequence.
ACCESSION   DAORNX010000532 DAORNX010000000
VERSION     DAORNX010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43326_length_1940_cov_6.423342"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /gene="modA"
                     /locus_tag="PLO65_16260"
     CDS             <1..333
                     /gene="modA"
                     /locus_tag="PLO65_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD29854.1"
                     /translation="YARAALEALGVWPSVEHRLLPGESVRAALAYVAREEAPLGVVYA
                     TDARAEPAVRLVGLFPAASHPPIRYPVALVAASRHRRAGALLDFLGGPEATAIFRRQG
                     FGAPPESD"
     gene            complement(330..857)
                     /locus_tag="PLO65_16265"
     CDS             complement(330..857)
                     /locus_tag="PLO65_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29855.1"
                     /translation="MSVRMSLLALSTLVLSGCDGVPLRTAERQVACCCDHCPTTAAPT
                     PPPREVEAPRRPPAARVYRAHSPPRPARESRYSGYSRSEEGRYEDGYAGRGRAYVGVS
                     VQESETSSARYSYSESSSGYAYGGAVATGGYGYGYSGAGPCCVGRPAPYPAPRYHGAS
                     TGPDGYLTWPGKVED"
     gene            complement(917..1282)
                     /locus_tag="PLO65_16270"
     CDS             complement(917..1282)
                     /locus_tag="PLO65_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29856.1"
                     /translation="MPPRPLPTLLFAIACGAVAWLCLTPAETLPPVSLWDKIEHALAY
                     LGLTLFGVWAFPRRLTRLATGLFCGGVAIEILQTLMGWGRQGDPADALANTVGIAAGL
                     LLTLAIRERIKVKSPARGE"
     gene            1384..>1940
                     /locus_tag="PLO65_16275"
     CDS             1384..>1940
                     /locus_tag="PLO65_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD29857.1"
                     /translation="MQVVTAVIWDFGGVITSSPFEAFNHFEAERGLPRDFIRRVNSTN
                     PHDNAWALFERNEIDAAAFDLKFLEEATALGHAVRGGDILPRLSGDVRPRMVAALKAC
                     KAVFKVGCITNNVQTGHGAGMAGTSGKAAQVAGIMELFDAVIESSKAGVRKPDPRIYQ
                     MMCELLDVEPADCVYLDDLGINCKPA"
BASE COUNT          277 a          665 c          724 g          274 t
ORIGIN      
        1 tacgcccggg ccgccctcga ggccctcggc gtctggcctt cggtcgagca tcggctgctg
       61 cccggcgagt cggtccgcgc ggcgctggcc tatgtcgccc gggaggaggc cccgctgggc
      121 gtggtctacg ccaccgacgc ccgggccgaa ccggccgtgc gcctggtggg tctgtttccg
      181 gcggcgagcc atccgccgat ccgctacccg gtcgccctgg tggcggcctc gcgccatcgc
      241 cgggcgggag ccttgctgga cttcctgggg ggaccggagg cgacggcgat cttccgccgc
      301 caaggctttg gcgccccgcc ggagtcagac tagtcctcga ccttgcccgg ccaggtcagg
      361 tagccgtccg ggccggtgct ggccccgtgg tagcgcggag ccggataggg ggccgggcgt
      421 ccgacgcagc acggtccggc gccgctgtag ccatagccgt agccgcccgt cgccacggcg
      481 ccgccatagg cgtagccgct cgaggattcg ctgtagctgt agcgcgccga actggtttcg
      541 ctctcctgca ccgacacgcc gacgtaggcg cggccacggc cggcgtagcc gtcctcgtag
      601 cggccctctt cggaccggct gtagccggag tagcggctct cgcgggccgg gcgcggcggg
      661 ctgtgcgccc gataaacgcg ggcggccggc ggccggcgcg gggcctcgac ctcgcgtggc
      721 ggcggcgttg gggcggcggt cgtcgggcag tggtcgcagc aacaggcgac ctggcgttcg
      781 gcggttcgaa ggggaacgcc gtcacagccg ctcagaacca gcgtcgacaa ggccagcagg
      841 gacatccgaa cggacatggg cctctccggg aagcaacagc cgctcaccct aggcggatcg
      901 cccgcccgtg gcccggctat tcaccccggg cgggagactt aaccttgatc cgttcgcgga
      961 tcgcgagcgt cagcagcagg cccgcggcga tgccgaccgt gttggccagg gcgtcggccg
     1021 ggtcgccctg gcgaccccag cccatcagcg tctgcaggat ttcgatggcc acgccgccgc
     1081 agaacaggcc ggtggccagc cgggtcaggc gtcggggaaa ggcccacacg ccgaacaggg
     1141 tcaggccgag ataggccagg gcgtgctcga tcttgtccca cagcgagacc ggcggcagcg
     1201 tctcggccgg cgtcaggcag agccaggcga ccgcgccgca ggcgatggcg aacagaaggg
     1261 tcggcagagg gcggggcggc atgcgggtaa ggcctgcggg ttgcgtcctg gtccggccgt
     1321 ctctagagcg cgttgatcgc gggatggaac aggtttcgcg cgggacggcg cgaaaaaggg
     1381 ggtgtgcagg tggtgacggc ggtgatctgg gacttcggcg gggtgatcac ctcctcgccg
     1441 ttcgaggcct tcaaccattt cgaggccgag cggggcctgc cgcgggactt catccggcgg
     1501 gtcaattcaa ccaatccgca tgacaacgcc tgggccctgt tcgagcgcaa cgagatcgac
     1561 gcggcggcct tcgacctgaa gtttctcgag gaggccaccg ccctcggcca cgccgtgcgg
     1621 ggcggcgaca tcctgccccg gctgtcgggc gacgtgcggc cgcgcatggt cgcggcgctc
     1681 aaggcctgca aggccgtgtt caaggtcggc tgcatcacca acaacgtcca gaccggccac
     1741 ggcgcgggca tggccggaac ctcggggaag gccgcccagg tggccgggat catggagctg
     1801 ttcgacgccg tgatcgaaag ctccaaggct ggcgtgcgca agccggaccc gcgcatctac
     1861 cagatgatgt gcgagctgct cgacgtcgag ccggccgact gcgtctacct cgacgacctg
     1921 ggcatcaact gcaagccggc
//
LOCUS       DAORNX010000533         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43327_length_1940_cov_5.587798, whole genome shotgun sequence.
ACCESSION   DAORNX010000533 DAORNX010000000
VERSION     DAORNX010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43327_length_1940_cov_5.587798"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..449
                     /locus_tag="PLO65_16280"
     tRNA            375..449
                     /locus_tag="PLO65_16280"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Asn,seq:gtt)
     gene            553..1572
                     /locus_tag="PLO65_16285"
     CDS             553..1572
                     /locus_tag="PLO65_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29858.1"
                     /translation="MTQPEPDPAQARKAVAGLYNALMTGLVLTLVSQRGERAAADFVF
                     AHFRRQHLEKFLPGLAKLGLDGLPDAVACAQYHLFSNALGGVKTELAVESDRKAWVRY
                     PPPRWIWQGTAICAVPASVNEAMLHGWHGHNGVSLGNPRLGFVCTGQTVLGDPGLEGY
                     YFEYDHDLADNERVRFAPDERCPAVDPAAQPRLDPAQWPAERLAKVEVSYAAEYVRSL
                     LPIMHGLFGPAEARALVGRTARLIGLQFHEPVMRALGLEDGSPTGFAMLLAALARAQG
                     EAVELDGPRVVQTGWRLMQGVEIDRTYALAAWNPLWEGALAAHNRHLRLETSGNGETI
                     IWTIR"
     gene            1586..>1940
                     /locus_tag="PLO65_16290"
     CDS             1586..>1940
                     /locus_tag="PLO65_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRD29859.1"
                     /translation="MTSPGFALFDTAIGRCGIAWGAAGIVGAALPGPDDDQMRRRFAE
                     RFPGATERDPPPGLRDVVAGVVALLAGEKVDLADAPLDLSATPPFHQRVYAVARRIPP
                     GQTMTYGEIAAALGGE"
BASE COUNT          284 a          689 c          677 g          290 t
ORIGIN      
        1 ggggcggctc ggcgcggggc ggcgcgcccg gggccagcag cacggccggt ccggccacca
       61 gggcggcggc gagaacaaga cctggaagga ggcgacggcg ggacatggga catcctgaat
      121 gtgccagcat ggtccgccgc tccgccgggg tcggccttga tcagcgtcaa tccacgccgg
      181 ccgccggccg ttaacgcctg attcaccatg cgggcgcacc ttcggccggt cttccgtgaa
      241 gacaccccac catcaccgac tttgaaagcc cggcctcacc cgccgggctc cttttcgtcg
      301 gctttgccgg atgcaaggcg cttgcgcccc accccggcca aagctatacg caccgacctt
      361 cctgagatgc gtgatccgcg gtagctcagt ggtagagcag ccgactgtta atcggctggt
      421 cgtaggttcg aatcctaccc gcggagccat cccccttccc catatcgccg ctgcggccgg
      481 ccccacccgc gggctgattg gcctgaacgc gggctggccc taggctgtcg tcgcatcgcg
      541 gaggggagcc acatgacgca gcccgagccg gaccccgccc aggcccgcaa ggccgtcgcc
      601 ggcctctaca acgccctgat gaccgggctg gtgctcacgc tggtcagcca gcgtggcgaa
      661 cgggccgcgg ccgacttcgt gttcgcccat ttccgccgcc agcatctgga gaagttcctg
      721 ccgggcctgg ccaaactggg gctggacggc ctgccggacg ccgtggcctg cgcgcagtac
      781 cacctgttct ccaacgccct gggcggggtg aagaccgaac tggcggtcga atcggaccgc
      841 aaggcctggg tgcgttatcc gccgccgcga tggatctggc agggcaccgc gatctgcgcc
      901 gtgccggcct cggtcaacga ggcgatgctg cacggctggc acggacacaa cggcgtgtcg
      961 ctcggcaatc cccgtctggg ctttgtctgc accggccaga cggtgctggg cgatccgggg
     1021 ctggaaggct actatttcga atacgaccat gaccttgcgg acaatgaacg ggtgcggttc
     1081 gcgcccgacg agcgttgtcc ggcggtggat ccggcggccc agcctcggct cgatcccgcg
     1141 caatggccgg cggagcggct ggcgaaggtc gaggtgtcct acgccgcgga atatgtgcgc
     1201 agcctgctgc cgatcatgca cggcctgttc ggcccggccg aagcgcgggc gctggtcggc
     1261 aggacggcgc gactgatcgg cctgcagttc catgagccgg tgatgcgggc gctgggactg
     1321 gaggacggct cgccgaccgg tttcgcgatg ctgctcgcgg ccctggcccg ggcgcagggc
     1381 gaggcggtgg aactcgacgg accgcgcgtc gtccagaccg gctggaggct gatgcagggt
     1441 gtcgagatcg acaggaccta cgccctcgcc gcctggaacc cgctgtggga gggggccctc
     1501 gccgcgcaca accgccatct ccggcttgaa acgtccggaa acggcgagac gatcatctgg
     1561 acgatccggt aagctgccca ccaccatgac ttcccccggc ttcgccctgt tcgacaccgc
     1621 catcggtcgc tgcggcatcg cctggggcgc ggccggcatc gtcggcgccg ccctgcccgg
     1681 ccccgatgac gaccagatgc gccggcgttt cgcagaacga ttcccggggg ccaccgagcg
     1741 cgatccgcca ccgggcctgc gcgatgtcgt cgccggcgtc gtggcgctcc tggccggaga
     1801 gaaggtcgat ctcgccgacg cgccgctcga cctgtcggcg acgccgccgt tccaccagcg
     1861 ggtctacgcc gtggcgcgca ggatcccgcc gggccagacc atgacctatg gcgagatcgc
     1921 cgcggcgctc ggcggcgaga
//
LOCUS       DAORNX010000534         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43426_length_1936_cov_8.990431, whole genome shotgun sequence.
ACCESSION   DAORNX010000534 DAORNX010000000
VERSION     DAORNX010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43426_length_1936_cov_8.990431"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLO65_16295"
     CDS             <1..204
                     /locus_tag="PLO65_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRD29860.1"
                     /translation="ARKRLAERLARLRAAGIGEADIAKLRAPIGLDLGGKAPWEVAVA
                     AIGEVVAVRRAFEGVQPRERVAL"
     gene            complement(205..489)
                     /locus_tag="PLO65_16300"
     CDS             complement(205..489)
                     /locus_tag="PLO65_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29861.1"
                     /translation="MNRRKTLLYSLAVGVGIAGVAISQWVMTHLKPSTELAVAYPAML
                     LGTGLIAGVLAYFADPGDPDRPTPSNDTDAKPEKVRVRVRRPGRRGEDRA"
     gene            complement(530..1009)
                     /gene="moaC"
                     /locus_tag="PLO65_16305"
     CDS             complement(530..1009)
                     /gene="moaC"
                     /locus_tag="PLO65_16305"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRD29862.1"
                     /translation="MSKLTHIDDQGRARMVDVTGKADTVREAVATGLVRMDAATRALA
                     LSGEAKKGDVRAVAEIAGVMAAKRTSELIPLCHPLALSKVEVSVTPHDDGLAVEARVR
                     TTGPTGVEMEALTAVSVACLTIYDMLKAADKGMIIEAVRLEEKTGGKSGEWKRLHTL"
     gene            complement(1006..1557)
                     /gene="moaB"
                     /locus_tag="PLO65_16310"
     CDS             complement(1006..1557)
                     /gene="moaB"
                     /locus_tag="PLO65_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HRD29863.1"
                     /translation="MTSPFPIGGRIDESLALRPVRVAVLTVSDTRDEESDTSGHLLAE
                     RVTSAGHELADKRMVRDDVAQIRQQVEAWVASGSVDAIVTTGGTGLTGRDVTPEAVRP
                     MLDKEIDGFSVIFHLVSYQSVGLSTLQSRALAGLVKGVFVFCLPGSNGAVRDGWDKVI
                     AAQLDSRHRPCNMVELMPRLLES"
     gene            complement(1558..>1936)
                     /locus_tag="PLO65_16315"
     CDS             complement(1558..>1936)
                     /locus_tag="PLO65_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRD29864.1"
                     /translation="AETGAVASFTGLARAEAGATTTLELEAWPGFTDIEIARIGKAAA
                     ARWGLHDWLVVHRTGPIAPGEAIVFVATAASHRREAFEACDHLMDYLKSRAPFWKKEH
                     GPDGARWVEPTDRDLTDAQRWET"
BASE COUNT          271 a          687 c          669 g          309 t
ORIGIN      
        1 gcccgcaagc ggctggccga gcggctggcg cggctgcggg cggcggggat cggtgaagcg
       61 gatatcgcga agctccgcgc gcccatcggc ctcgacctcg gcggcaaggc gccctgggag
      121 gtggcggtgg cggcgatcgg ggaggtggtg gccgtcaggc gagcgttcga aggcgtccag
      181 cccagggaac gggtcgccct ctgatcaggc ccgatcctcg ccccggcgac ccggccgacg
      241 aaccctgacc ctgacttttt caggcttggc atcggtatca ttcgacggcg tgggccggtc
      301 aggatcgccc ggatcggcga agtaggcgag aacgcccgcg atcaatccgg ttcccaacag
      361 catggcggga taggcgaccg ccagttcggt cgacggcttg aggtgggtca tgacccactg
      421 gctgatcgcg acgccagcaa tgccgacgcc taccgccagg ctgtacaaca gcgtcttgcg
      481 tcggttcacg ccgccctctc cctgatgccg tttcgcaagg ctttgtcctt catagcgtat
      541 gcagccgctt ccactcgccc gacttgccgc cggtcttttc ctccagccgc acggcctcga
      601 tgatcatgcc tttgtcggcc gccttgagca tgtcgtagat cgtcaggcag gcgaccgaga
      661 cggcggtcag ggcctccatc tcgaccccgg tcgggccggt ggtcctgacc cgcgcctcga
      721 cggccaggcc gtcgtcgtgc ggcgtcaccg acacctcgac cttcgacagg gccagcggat
      781 ggcacagcgg gatcagctcg ctcgtccgct tggccgccat cacgccggcg atctcggcca
      841 cggcccgcac gtcgcccttc ttcgcttcgc ccgacagggc cagggcgcgg gtcgccgcgt
      901 ccatcctgac cagacccgtg gcgaccgcct cacgcaccgt gtcggccttg cccgtgacat
      961 cgaccatccg cgcacggccc tggtcgtcga tgtgggtgag cttgctcatg actcgagcag
     1021 cctcggcatc agctcgacca tgttgcaggg gcggtggcgg ctgtcgagct gggccgcgat
     1081 gaccttgtcc cagccgtccc tgaccgcgcc gttggagccg ggcaggcaga acacgaacac
     1141 gcccttcacc aggccggcca gggcccgcga ctgcagggtg gagaggccga ccgactggta
     1201 gctgaccaga tggaagatca ccgagaagcc gtcgatctcc ttgtccagca tcggccgcac
     1261 ggcctcgggc gtcacgtcgc ggccggtcag gccggtgccg ccggtggtga cgatggcgtc
     1321 gaccgagccc gacgcgaccc aggcctcgac ctgctgacgg atctgggcca cgtcgtcgcg
     1381 caccatccgc ttgtcggcca gttcgtggcc cgctgacgtc acgcgctcgg ccagcaggtg
     1441 gccggaggtg tcgctctcct cgtcacgggt gtccgagacg gtcagcacgg cgacgcgcac
     1501 gggcctgagg gccaggcttt cgtcgatcct gccgccgatg gggaagggcg aggtcattca
     1561 ggtctcccag cgttgggcgt cggtcaggtc acggtcggtc ggctcgaccc agcgggcgcc
     1621 gtccggtccg tgctccttct tccagaaggg cgcgcggctc ttgagatagt ccatcagatg
     1681 atcgcaggcc tcgaacgcct cgcgccggtg cgaggccgcg gtcgccacga agacgatcgc
     1741 ctcgccgggt gcgatcggac cggtgcgatg cacgaccagc cagtcgtgca gaccccagcg
     1801 cgcggcggcg gccttgccga tgcgggcgat ctcgatgtcg gtgaaccccg gccaggcctc
     1861 aagctccagc gtcgtcgtcg cgccggcctc ggcccgggcc aggccggtga agctggccac
     1921 ggccccggtc tcggcg
//
LOCUS       DAORNX010000535         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43427_length_1936_cov_8.900053, whole genome shotgun sequence.
ACCESSION   DAORNX010000535 DAORNX010000000
VERSION     DAORNX010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43427_length_1936_cov_8.900053"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLO65_16320"
     CDS             complement(<1..190)
                     /locus_tag="PLO65_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD29865.1"
                     /translation="MTAPFDDEEIEAARVLFAHPCSFMMGAVKMDGLPPADLPEVAFA
                     GRSNVGKSSLINALVGQKY"
     gene            complement(187..>1936)
                     /gene="yidC"
                     /locus_tag="PLO65_16325"
     CDS             complement(187..>1936)
                     /gene="yidC"
                     /locus_tag="PLO65_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRD29866.1"
                     /translation="TVRVSREAAKAASPRVAIATPSLTGSLRLQGARIDDLFLTARNA
                     NGLKYGETLKKDSPPVELLRPEGAEHAWFADFGWTDEIAPGVPTPKVPGLPTPDTVWT
                     VTSANTVLSPGKPVTLTYVAPNRLVFTRTVAVDDKFMFTVTDTVSNLGAAPVTIRPYA
                     SVQRQGLPPASKAQNVHEGAVGALGDLPDKADAQLKLLKFRNWKKEAGKDADRARAAA
                     ARAGRVYDPNSEVIVDSYSSDGGWLGLTDKYWLAALVPAQSERITGQYGLVSTQGVDI
                     YAAHFGGTPRIVNPGRQITTTTRLFAGAKEVQTLKGYQKAFNIPRFEDAVDWGVLSFL
                     TKPFFLALEFFYSHLGNFGLAILAMTVCVRLLVFPLAQKSSESMARMKKLQEPMNEIK
                     ARHKDDPVKAQQETMALMQREKVNPMAGCLPILVQFPVFLAFYKVLSVTIEMRHAPFV
                     GWLHDLSAQDPTTILNLFGLLPYDPAQLPVIGGLLAQYLHIGVLPLLYGFTMWLVTAM
                     NPPAPDPMQQRIFQLMPIMFTFIMAGFPAGLLVYWTFSNVFSIFQQYVIMHRLKTENP
                     IDTFLARLRGEDKATA"
BASE COUNT          302 a          612 c          703 g          319 t
ORIGIN      
        1 ggtacttctg gccgaccagg gcgttgatca ggctcgactt gccgacgttg gagcggccgg
       61 cgaacgccac ctccggcagg tcggccggcg gcaggccgtc catcttcacc gcccccatca
      121 tgaagctgca gggatgggcg aagaggaccc gggcggcctc gatctcctcg tcgtcgaacg
      181 gcgccgtcac gccgtcgcct tgtcctcacc gcgaaggcgg gcgaggaagg tgtcgatggg
      241 gttctcggtc ttcagccggt gcatgatcac gtactgctgg aagatcgaga acacgttgga
      301 gaaggtccag tagaccagca ggcccgccgg gaagccggcc atgatgaagg tgaacatgat
      361 cggcatcagc tggaagatcc gctgctgcat cgggtcgggc gccggcgggt tcatcgccgt
      421 caccagccac atggtgaagc cgtagagcag cggcagcacg ccgatgtgca ggtactgggc
      481 gagcaggccg ccgatgaccg gcagctgggc cggatcgtag ggcagcaggc cgaacaggtt
      541 gaggatggtc gtcgggtcct gggccgacag gtcgtgcagc cagccgacga acggcgcatg
      601 gcgcatctcg atagtcaccg acagcacctt gtagaaggcc aggaacaccg ggaactgaac
      661 gaggatcggc aggcagcccg ccatcgggtt gaccttctcc cgctgcatca gggccatggt
      721 ctcctgctgg gccttgaccg ggtcgtcctt gtggcgggcc ttgatctcgt tcatcggctc
      781 ctggagcttc ttcatccggg ccatcgactc ggagctcttc tgggccaggg ggaagaccag
      841 caggcgcacg cagacggtca tggccaggat cgccagtccg aagttgccca ggtggctgta
      901 gaagaactcc agcgccagga agaacggctt ggtcaggaag ctcagcaccc cccagtcgac
      961 ggcgtcctcg aagcgcggga tgttgaacgc cttctggtag cccttcagcg tctggacttc
     1021 cttggcgccg gcgaacaggc gcgtggtcgt ggtgatctgg cggcccgggt tgacgatgcg
     1081 cggcgtgccg ccgaagtgcg cggcgtagat gtcgacgccc tgggtgctga ccaggccgta
     1141 ctggccggtg atgcgctccg actgggccgg cacgagggcc gcgagccagt acttgtcggt
     1201 caggccgagc cagccgccgt ccgaactgta gctgtcgacg atcacttcgc tgttgggatc
     1261 atagacccgt ccggcgcggg cggccgcggc gcgggcccga tcggcgtcct tgccggcttc
     1321 cttcttccag ttgcggaact tgagcagctt cagctgcgca tcggccttgt caggcaggtc
     1381 gcccagcgcg cccaccgcgc cctcgtggac gttctgggcc ttgctggccg gcggcaggcc
     1441 ctggcgctgc acggaggcgt aggggcggat ggtgaccggc gccgcgccga ggttggagac
     1501 cgtatcggtc accgtgaaca tgaacttatc gtcgaccgcc accgtgcggg tgaacaccag
     1561 gcggttgggg gcgacgtagg tcagggtcac cggcttgcct ggcgacagga ccgtattggc
     1621 cgaggtgacc gtccagacgg tgtcgggagt gggcaggccc gggaccttgg gggtcggcac
     1681 gccgggggcg atctcgtcgg tccagccgaa gtcggcgaac caggcgtgct cggcgccttc
     1741 gggccgcagc agctcgaccg gcggcgagtc cttcttcagc gtctcgccgt acttgagccc
     1801 attggcgttg cgcgcggtca ggaacaggtc gtcgatccgg gcgccctgca ggcgcagcga
     1861 gccggtcagc gacggcgtgg cgatggcgac ccgggggctg gccgccttgg ccgcctcgcg
     1921 cgagaccctg accgtc
//
LOCUS       DAORNX010000536         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43751_length_1924_cov_8.295880, whole genome shotgun sequence.
ACCESSION   DAORNX010000536 DAORNX010000000
VERSION     DAORNX010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43751_length_1924_cov_8.295880"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLO65_16330"
     CDS             complement(<1..667)
                     /locus_tag="PLO65_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRD29867.1"
                     /translation="MAVLVVADNDNAHVRDTTNKTVTAALAMSGDVDVLVLGQGADAA
                     AAAAAKISGVRKVILAEGADVGHGLAEAISDTVAGLMGGYDAVLVPATSAGKNFAPRV
                     AAKLDASPISDVVEVVSADTFVRPIYAGNAMETVQTGDAKKVLTVRPTAFKAADNGGS
                     ASVEKTAAAAGSGAKFVSEEMVKSDRPELTAAKIVVSGGRAMGSAEEFQRVIEPLADK
                     LGAA"
     gene            complement(667..1416)
                     /locus_tag="PLO65_16335"
     CDS             complement(667..1416)
                     /locus_tag="PLO65_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRD29868.1"
                     /translation="MKVLVPVKRVIDYNVKARVKPDQSGVDLANVKMSMNPFCEIAVE
                     EAVRLKEKGVATEVVAVSVGPTQAQETIRTALAMGADRGVLIQSDVDVEPLAVAKLLA
                     AVAKEEGADVVLMGKQAIDGDNNAVGQMLSQLLGWPQATFASKVEIAGGKATVTREVD
                     GGLQVLEVALPAVITADLRLNEPRYASLPNIMKAKKKEIAMKATGDYGVDVAPRLKVL
                     KVTEPAKRSAGIKVETAAELVSKLKTEAGVL"
     gene            1565..1885
                     /locus_tag="PLO65_16340"
     CDS             1565..1885
                     /locus_tag="PLO65_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29869.1"
                     /translation="MNPTINRLKLLFLVLFAIGTVGIWAYQIFYVWPAHRCDEQGRWW
                     DRKGRVCATPIYIPSITGRPKGMSRKEWSEKQAAREIQREQEGYPTTEAASAPPAPAA
                     AAKK"
BASE COUNT          291 a          659 c          643 g          331 t
ORIGIN      
        1 ccgccgcgcc gagcttgtcg gccagcggct cgatgacccg ctggaattcc tcggccgagc
       61 ccatggcgcg accgccggag acgacgatct tggcggcggt cagttccgga cggtccgact
      121 tgaccatttc ctcggagacg aacttcgcgc ccgaaccggc ggcggcggcc gtcttctcga
      181 ccgaggcgga gcccccgttg tcggcggcct tgaaagcggt cgggcgaacc gtcagcacct
      241 tcttggcgtc gccggtctgg accgtctcca tggcgttgcc ggcatagatc ggccgcacga
      301 aggtgtcggc gctgaccact tcgacgacat ccgagatcgg cgaggcgtcg agcttcgccg
      361 ccacgcgcgg ggcgaagttc ttgccggcgg aggtggccgg gaccagcacc gcgtcgtagc
      421 cgcccatcag gccggcgacg gtgtcggaga tggcttcggc caggccgtga cccacgtccg
      481 cgccctcggc caggatgacc ttgcgcacgc cggagatctt ggcggccgcg gcggccgcgg
      541 cgtcagcgcc ctggcccagg accagaacgt cgacatcgcc cgacatcgcc agggcggcgg
      601 tgacggtctt gttggtggtg tcgcgcacat gcgcgttgtc gttatcggcg acgacgagaa
      661 cggccatcag aggacccccg cttcagtctt gagcttgctc accagttcgg cggcggtttc
      721 gaccttgatg ccggccgagc gcttggccgg ctcggtgacc ttcaggacct tcagccgcgg
      781 cgcaacatcg accccgtagt cgccggtcgc cttcatggcg atttccttct tcttcgcctt
      841 catgatgttc ggcagagaag cgtagcgcgg ctcgttcagg cgcaggtcgg cggtgatcac
      901 cgccggaagg gccacttcga gaacctgcag gccgccgtcg acctcgcggg tcacggtggc
      961 cttgccgccg gcgatctcga ccttcgaggc gaaggtggcc tgcggccagc ccagcagttg
     1021 cgagagcatc tggcccacgg cgttgttgtc gccgtcgatg gcctgcttgc ccatcagcac
     1081 cacgtcggcg ccctcttcct tggcgacggc ggcgagcagc ttggccaccg ccagcggctc
     1141 gacatcgacg tccgactgga tcaggacgcc gcggtcggcg cccatggcca gggccgtgcg
     1201 gatggtttcc tgcgcctggg tcgggccgac ggagaccgcg acgacctcgg tggcgacgcc
     1261 cttttccttg agacggacgg cctcttcgac cgcgatctcg cagaagggat tcatggacat
     1321 cttgacgttg gccaggtcca cgcccgactg gtcgggcttc acccgagcct tcacgttgta
     1381 atcgataacc cgtttgaccg ggaccagcac cttcatgggt ttgtccgccc gtgtttgttg
     1441 cgttgcgaaa atttttgcgc ggggaaggct gataacgact ccgtcgcgca atgcaagttt
     1501 acctagaggt caatccggcg ccgtcaaatt gccgtcgaag cccccttcgt atagacggcg
     1561 cgccatgaac ccgacgatca accgcctcaa gctcctgttc ctggtcctgt tcgccatcgg
     1621 cacggtgggg atctgggcct accagatctt ctacgtctgg ccggcccacc ggtgtgacga
     1681 gcagggccgg tggtgggacc gcaagggccg ggtctgcgcc acgccgatct acatccccag
     1741 catcaccggc cggcccaagg gcatgagccg caaggaatgg tcggagaagc aggctgcccg
     1801 cgaaatccag cgcgagcagg aaggctatcc gacgaccgag gccgcttcgg cgcccccggc
     1861 gccggcggcc gccgcgaaga agtagccagg tcgcggcgtc ctcgcacgcg ccccgagccg
     1921 gccg
//
LOCUS       DAORNX010000537         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_43876_length_1920_cov_7.436461, whole genome shotgun sequence.
ACCESSION   DAORNX010000537 DAORNX010000000
VERSION     DAORNX010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43876_length_1920_cov_7.436461"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="PLO65_16345"
     CDS             <1..1884
                     /locus_tag="PLO65_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD29870.1"
                     /translation="ASAGGGGVAQASTVREAFGVPASDLKADLLVRGRRPNGSTVDIA
                     SRPVAGGQLQAMAAAAPLGVVEANRDRIDGVVSLLVPLGIGIALALLLFSQSRRAEAA
                     QRAFVESEQRFRMAVEAARCGIWEWDLESDQVFMSDVTGVIFGWGGGGVVGGQEVLDR
                     VAPDHRDRVRQALVHAASFGAFDVSFRVPDRQGGKSIWIDARGQGLGQSDEAGYHRII
                     GVALDVTEERLAQARAQAAENRLRDAIESVSEAFVLWDRSGRLLLCNRNYRSVFNLEP
                     KVLKPGAARDHVNRFVQLAIKQEIAGPEGMRGVREAELMDGRWVQISERRTAEGGLVM
                     TATDISAVKAQEEARRLNEEQLQNAVVSLERSQEQLAELARKYENEKVRAEAANKAKS
                     EFLANMSHELRTPLNAVNGFSEIMIAEMYGPLGDPRYKEYATDIHNSGQHLLALINDI
                     LDMSKIEAGKMNLKFEPLNLEEVTEDAVRLVRNRAEAAGLFLTVDFPFLPEIEADYRA
                     VKQVLLNLLSNAIKFTPRGGRVTVRAEVRQDTLGERVRVSVSDTGIGISKDDLARLAQ
                     PFEQVESQHSKTTQGTGLGLALTKSLIELHEGALEMESTPGEGTTVSFILPIRRLPGV
                     IAA"
BASE COUNT          317 a          663 c          674 g          266 t
ORIGIN      
        1 gccagtgctg gcggcggggg cgtagcccag gcatcgacgg tgcgggaggc cttcggcgtg
       61 ccggcctcgg acctcaaggc cgaccttctg gtgcgcggtc ggcgtcccaa cgggtcgaca
      121 gtggacatcg ccagccgccc ggtcgcgggc ggccagctgc aggcgatggc cgcggccgcg
      181 cctctgggcg tggtcgaggc caaccgcgac cgcatcgacg gggtggtctc gctgctggtg
      241 ccgcttggca tcggcatcgc cctggccctg ctgctgttct cccagagccg ccgcgccgaa
      301 gcggcccagc gcgcctttgt cgagagcgag cagcggttcc gcatggcggt cgaggccgcc
      361 cgttgcggca tctgggaatg ggacctggaa agcgaccagg tgttcatgtc cgacgtcacc
      421 ggcgtgatct tcggctgggg cggcggcggc gtggtcggcg ggcaggaggt gcttgaccgc
      481 gtcgcccccg atcatcgcga ccgcgtgcgc caggcgctgg tccacgcggc cagtttcggg
      541 gccttcgatg tgtccttccg cgtgccggac cgccagggcg gcaagtcgat ctggatcgac
      601 gcgcgcggcc agggcctcgg ccagtccgac gaggccggct atcaccggat catcggcgtg
      661 gcgctggacg tcaccgagga gcggctcgcc caggcccgcg cccaggctgc cgagaaccgt
      721 ctgcgggacg cgatcgagag cgtctcggaa gccttcgtcc tgtgggaccg ttcaggccgc
      781 ctgctgctgt gcaaccgcaa ctatcgcagc gtgttcaacc tcgaacccaa ggtgctcaaa
      841 cccggcgccg cccgcgacca cgtcaaccgc ttcgtccagc tggctatcaa gcaggagatc
      901 gccggcccgg aggggatgcg cggcgtgcgc gaggccgagc tgatggacgg gcgctgggtg
      961 cagatctccg aacgccgcac ggccgagggc gggctggtca tgaccgccac cgacatctcc
     1021 gccgtcaagg cgcaggagga ggcgcgccgg ctcaacgagg agcagctgca gaacgccgtc
     1081 gtcagcctcg agcgcagcca ggagcagctg gccgagctgg cccgcaagta cgagaacgag
     1141 aaggtccggg ccgaggccgc caacaaggcc aagagcgagt tcctcgccaa catgtcgcac
     1201 gagctgcgca cgccgctcaa cgcggtcaac ggcttcagcg agatcatgat cgccgagatg
     1261 tacggaccgc tgggcgatcc ccgctacaag gaatacgcca ccgacatcca caacagcggc
     1321 cagcacctgc tggccctgat caacgacatt ctcgacatgt cgaagatcga ggccggcaag
     1381 atgaacctca agttcgagcc gctgaacctc gaggaggtca ccgaggacgc cgtgcgactg
     1441 gtgcgcaacc gcgccgaggc ggccgggctg ttcctgaccg tcgacttccc cttcctcccc
     1501 gagatcgagg ccgactaccg ggcggtcaag caggtgctgc tgaacctgct cagcaacgcg
     1561 atcaaattca ccccccgggg cggccgtgtc acggtccgcg ccgaggtccg ccaggacacc
     1621 ctgggcgagc gcgtgcgtgt ttcggtgtcg gataccggca tcggcatcag caaggacgat
     1681 ctggctcgac tggcccagcc gttcgaacag gtcgagagcc agcacagcaa gaccacccag
     1741 ggcacgggcc tgggcctggc cctgaccaag tcgctgatcg agctgcatga gggcgcgctg
     1801 gaaatggaaa gcacgcccgg cgaaggcacc accgtcagct tcatcctgcc gatccggcgg
     1861 ctgccggggg tcatcgcggc ctgaaccgcc cgcgcgcggc gcggttcagg gagcgccgcc
//
LOCUS       DAORNX010000538         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44018_length_1914_cov_5.982249, whole genome shotgun sequence.
ACCESSION   DAORNX010000538 DAORNX010000000
VERSION     DAORNX010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44018_length_1914_cov_5.982249"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="fliP"
                     /locus_tag="PLO65_16350"
     CDS             <1..635
                     /gene="fliP"
                     /locus_tag="PLO65_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080852.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRD29871.1"
                     /translation="RVVQLIGLLTVLSLAPSIVIMTTSFVRITVVLSLLRTALGLQQS
                     PPNAVLISLALFLTAIVMGPTLQRSYDEGVRPLLDKQMELPQAFEAAGDPVKQFMLAQ
                     VDREDLALFVRLSKIDKPATLQDTPIRVVTPAFMISELKRAFEIGFLLFVPFLVIDLV
                     VASVLMSMGMMMLPPVVVSLPFKLIFFVLVDGWRLVAGSLVESFHQIPPG"
     gene            complement(666..>1914)
                     /locus_tag="PLO65_16355"
     CDS             complement(666..>1914)
                     /locus_tag="PLO65_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HRD29872.1"
                     /translation="ESLPAADQLAVRLIQARLFEAEGRKKGALNVYLAIARAPLDQLA
                     APALLRATRIKQELGQITPAQAAMAYDGLRYRWRGDATELETIRTLGQLYLGQGRYRE
                     ALEALRSAGTRLPDLPQAVQLQADLANAFRALFLDGMADGLEPIQSLALFYDFQELTP
                     PGADGDLMVRKLVRRLVDVDLLTDAGKLLQYQVENRLDGVPKAQVATDLALIYLMDRK
                     PEQALAAINGSRTTVLPAALNSDRRIITARALMALGRLDAAAEIIEPDTGPDAQDVRA
                     EIAWKGHDWALAGSLFERTLADRWKSVLPLRPDEESKLLRAGVAYSLAGDDVSLARLR
                     EHFAGYVEQARNPEALRVAFAGVDGGALTANDFSRTVADNEGFAGWVAKMKQRFRDKP
                     APVGPAVTRAAAAPAAAPATPRG"
BASE COUNT          261 a          662 c          686 g          305 t
ORIGIN      
        1 aacgggtggt gcagctgatc ggcctgctga ccgtgctgtc gctggccccg tcgatcgtca
       61 tcatgaccac cagcttcgtg cgcatcacgg tggttctgag cctgctgcgc acggcgctgg
      121 gcctgcagca gagcccgccg aacgcggtgc tgatcagcct ggccctgttc ctgaccgcca
      181 tcgtcatggg cccgaccctg cagcgtagct atgacgaggg cgtgcggccc ctgctcgaca
      241 agcagatgga gctgccacag gcgttcgagg cggccggcga cccggtcaag cagttcatgc
      301 tggcccaggt cgaccgggag gacctggccc tgttcgtacg gctgtcgaag atcgacaagc
      361 ccgcaaccct gcaggacacg ccgatccgcg tggtcacccc ggccttcatg atcagcgagc
      421 tgaagcgcgc gttcgagatc ggcttcctgc tgttcgtgcc gttcctggtc atcgacctgg
      481 tggtggccag cgtgctgatg agcatgggca tgatgatgct gccgccggtg gtggtcagcc
      541 tgccgttcaa gctgatcttc ttcgtgctgg tcgacggctg gcggctggtc gccggcagcc
      601 tggtcgagag cttccaccag atcccgcccg ggtagatcgc atccctcccc gggatgggag
      661 ggcgcctagc ccctcggcgt cgccggcgcc gcggccggag cggccgccgc ccgcgtcacc
      721 gccggtccga ccggggcggg tttgtcgcgg aagcgctgct tcatcttcgc cacccaaccg
      781 gcgaagcctt cattgtcggc gacggtgcgg ctgaaatcat tggccgtcag ggccccgcca
      841 tcgacgccgg cgaaggcgac ccgcagggcc tccggattgc gcgcctgttc gacatagccg
      901 gcgaagtgct cgcgcagccg cgccagggat acgtcgtcgc cggccaggct gtaggccacg
      961 cccgcgcgca gcagcttcga ctcctcgtcc gggcgcagcg gcaggacgct cttccagcgg
     1021 tccgccaggg tccgctcgaa cagcgaaccg gccagcgccc agtcatggcc cttccaggcg
     1081 atctccgcgc gcacgtcctg ggcgtcgggg ccggtgtccg gctcgatgat ctcggcggcg
     1141 gcgtcgagcc ggcccagcgc catcagggcg cgggcggtga tgatccgtcg atcgctgttg
     1201 agcgctgcgg gcaggaccgt ggtgcgcgaa ccattgatcg ccgccagcgc ctgctcgggc
     1261 ttgcggtcca tcaggtagat cagcgccagg tcggtggcga cctgggcctt gggcacgccg
     1321 tcgaggcggt tctcgacctg gtactgcagc agcttgccgg cgtcggtcag cagatcgacg
     1381 tcgaccagcc ggcggaccag cttgcgcacc atcaggtcgc cgtcggcccc gggcggggtc
     1441 agttcctgga agtcgtagaa cagggccagc gactggatcg gctcgagacc gtcggccatg
     1501 ccgtcgagga acagggcgcg gaaggcgttg gccaggtccg cctgcagctg caccgcctgg
     1561 ggcaagtcgg gcaggcgcgt gccggccgac cgcagggcct caagcgcctc gcgataccgg
     1621 ccctggccca ggtagagctg gccgagggtg cggatggtct ccagctcggt cgcgtcgccg
     1681 cgccagcggt agcgcaggcc gtcataggcc atcgccgcct gggccggggt aatctgaccc
     1741 agttcctgct tgatccgggt ggcccgcagc agggccgggg cggccagctg gtcgagcggc
     1801 gcgcgggcga tggccagata gacgttcagg gcgcccttct tgcgcccctc ggcctcgaag
     1861 agccgcgcct ggatcagccg cacggccagc tggtcggcgg cgggcagcga ctcg
//
LOCUS       DAORNX010000539         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44196_length_1907_cov_5.303456, whole genome shotgun sequence.
ACCESSION   DAORNX010000539 DAORNX010000000
VERSION     DAORNX010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44196_length_1907_cov_5.303456"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PLO65_16360"
     CDS             complement(<1..140)
                     /locus_tag="PLO65_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29873.1"
                     /translation="MSRIASVLLPMPLPEAFDYAEPEGMGLAVGDHVAVPLGPRAVRG
                     VVT"
     gene            180..833
                     /gene="fsa"
                     /locus_tag="PLO65_16365"
     CDS             180..833
                     /gene="fsa"
                     /locus_tag="PLO65_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRD29874.1"
                     /translation="MQLFLDTTDTVVLKDLAATGLVDGVTTNPTLIAKSGRPMAEVIA
                     EICGLVEGPVSAEVAAMDVEGMLAEGRKLAAIATNVVVKVPLTRNGLVATSEFAVEGI
                     STNVTLCFSAAQALLAAKAGASYISPFIGRLDDHGAVGMDLIGEIRAIYDNYGFDTEI
                     LAASIRTPAHVTAAALAGADCATIPPATFEALFKHPLTDKGLEQFMADWAKTGQSIL"
     gene            888..1556
                     /locus_tag="PLO65_16370"
     CDS             888..1556
                     /locus_tag="PLO65_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRD29875.1"
                     /translation="MSAARQPESAADELRRVLRAHPELIRDDPELLAALGLRVDSANL
                     VDFGPAALARVNAAHRREATVRRKLEAMARANFSAQAQTHAAVIDILESRNHSDLARR
                     VDDLASLRFGLVAATIAIEGPGRTPAGWHALVEGQSDLILGPQRLSRMGFHAPARGLF
                     GERADQVRSMAMVRIAIWEPGREGVLAFGSPDAKGFTADMGAELVAFLARVVERTAER
                     WPVL"
     gene            1654..>1907
                     /locus_tag="PLO65_16375"
     CDS             1654..>1907
                     /locus_tag="PLO65_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD29876.1"
                     /translation="MTTARDAMAAWLDHLANERRVSPRTIEAYLGAVSPYISFLEQHR
                     GGALSAADMAGVTPAELRAYLAHRRDGARPLSARSVSQALS"
BASE COUNT          281 a          716 c          638 g          272 t
ORIGIN      
        1 gtgaccacgc cgcgcacggc ccgcgggccc agcggcacgg cgacgtggtc gccgacggcc
       61 aggcccatgc cctcgggctc ggcgtagtcg aacgcctcgg gcagcggcat gggcagcagg
      121 acggacgcga ttcggctcac gggtggcgcc ccgcgatccg cctcgctaaa accccgctca
      181 tgcaactgtt cctcgacacc accgacaccg tcgtcctcaa ggacctcgcc gccaccggcc
      241 tcgtggacgg cgtgaccacc aatcccacgc tgatcgccaa gtccggccgg cccatggcgg
      301 aggtcatcgc cgagatatgc ggtctcgtcg agggcccagt gagcgccgag gtcgcggcga
      361 tggacgtcga gggcatgctg gccgaaggcc gcaagctcgc cgccatcgcc accaatgtgg
      421 tggtcaaggt gccactgacc cgcaacggcc tggtcgccac cagcgaattc gcggtcgagg
      481 gcatctcgac caacgtcacc ctgtgcttct ccgccgccca ggcgctgctc gcggccaagg
      541 cgggcgccag ctacatctcg ccgttcatcg gccggctcga cgaccacggc gcggtcggca
      601 tggacctgat cggcgagatt cgcgcgatct acgacaacta cggcttcgac accgagatcc
      661 tcgccgcctc gatccgcacc ccggcccatg tgaccgccgc ggccctggcc ggggcggact
      721 gcgcgaccat tcccccggcc accttcgagg ccctgttcaa gcacccgctg accgacaagg
      781 gtcttgagca gttcatggcc gactgggcga agacgggcca gtcgatcctc tgaacggatt
      841 ggtaggaggt ggccgctagc gtctcccggt tgattcggga gacgatcatg agcgcggcaa
      901 gacaaccgga atcggcggcc gacgagcttc gccgcgtgct gcgcgcccac cccgagctga
      961 ttcgcgacga cccagagttg ctggccgccc tgggcctgcg cgtcgactcg gccaacctcg
     1021 tcgacttcgg ccccgccgcc ctggcccggg tcaatgccgc gcacaggcgc gaagccacgg
     1081 tgcgtcgcaa gctggaagcc atggcgcggg ccaacttctc cgcccaggcc cagacccatg
     1141 cggcggtgat cgacatcctc gagtcgcgca accattccga cctcgcgcga cgggtcgacg
     1201 acctggccag cctgcggttc gggctggtcg ccgcgaccat cgccatcgag ggcccgggcc
     1261 gcacgccggc cggctggcac gcgctggtcg agggccagag cgacctgatt ctcggtccgc
     1321 agcgcctgtc gcggatgggc ttccacgccc cggcgcgggg cctgttcggc gaacgggccg
     1381 accaggtgcg ctcgatggcc atggtccgca tcgcgatctg ggagcccgga cgcgagggcg
     1441 tgctggcgtt cggctcgccc gacgccaagg gcttcacggc cgacatgggc gccgagctgg
     1501 tcgccttcct cgcccgggtc gtcgagcgga cggcggaacg atggccggtg ctgtgatcga
     1561 tttgccgggg acatgtaccc aacgggtacg tgtcccctac cccggccgct gggacagggg
     1621 acacgtacct tcggtacatg tccccggatc gccatgacca ccgcccgcga cgccatggcc
     1681 gcctggctcg accacctggc caacgagcgc cgcgtctcgc cgcggacgat cgaggcctat
     1741 ctcggcgcgg tcagccccta catcagcttc cttgagcaac atcgcggcgg cgccctgtcg
     1801 gccgccgaca tggccggcgt caccccggcc gaactgcgcg cctatctggc gcatcggcgc
     1861 gacggcgcgc ggccgctctc ggcgcgctcg gtgtcgcagg ccctgtc
//
LOCUS       DAORNX010000540         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44512_length_1895_cov_8.455978, whole genome shotgun sequence.
ACCESSION   DAORNX010000540 DAORNX010000000
VERSION     DAORNX010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44512_length_1895_cov_8.455978"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /gene="ligA"
                     /locus_tag="PLO65_16380"
     CDS             complement(<1..1566)
                     /gene="ligA"
                     /locus_tag="PLO65_16380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRD29877.1"
                     /translation="MTLTPVPDLTESEAAEELERLADELAAHDLRYHQEDAPTISDAD
                     YDALKRRNEAIEARFPHLIRDNSPSLRVGAARSEQFAPVEHGVPMLSLDNAFSDEEVE
                     DFVARVRRFLRLAADEPVAFTAEPKIDGLSASIRYEKGVLVRGATRGDGRTGEDITAN
                     LRTLDDIPERLKGQVPDVIEVRGEVYAPLAEFEAFNRAAEEAGTRTYANPRNFAAGSL
                     RQIDPAVTARRPLRFFAYAWGEVSAPFADTQSGALERLADWGFATNDRSRRVENADGL
                     LGVYRDIGAARPTLAYDIDGVVYKVDRLDWQQRLGFVSRSPRWAIAHKFPAQQATTVL
                     EDIDIQVGRTGSLTPVARLHPVTVGGVVVRNATLHNADEIARKDIRIGDTVTLQRAGD
                     VIPQILGPVDADRPGRGAPYVFPTTCICPLKTPVVQETTAGGAITVARRCTGEFACPF
                     QKIRHLMHFVSRRAFDIEGLGEKQLQAFLDEGWITAPADIFRLARDEDRLALLREREG
                     YGETSVANIVRNVD"
     gene            complement(1694..>1895)
                     /locus_tag="PLO65_16385"
     CDS             complement(1694..>1895)
                     /locus_tag="PLO65_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRD29878.1"
                     /translation="VVTHSPQVAARAHAHWRISKGGDETRIRTAVETLSPAAREEEIA
                     RMLAGAEITDAARAAARALIGA"
BASE COUNT          260 a          658 c          679 g          298 t
ORIGIN      
        1 gtcgacgttg cgcacgatgt tcgccaccga ggtttcgcca tagccctcgc gctcgcgcag
       61 cagggccagc cggtcctcgt cacgcgccag gcggaagatg tcggccggcg cggtgatcca
      121 gccctcgtcg aggaaggcct gcagctgctt ctcgcccagg ccctcgatgt cgaaggcccg
      181 ccgcgagacg aagtgcatca ggtggcgaat cttctggaac ggacaggcga actcgcctgt
      241 gcagcgccgc gccacggtga tcgccccacc ggccgtcgtc tcctgcacca cgggcgtctt
      301 cagcggacag atgcaggtgg tcgggaagac gtagggcgcg ccgcgtccgg gccggtcggc
      361 gtcgaccggc ccgagaatct gcgggatcac gtcgccggcc ctttgcaggg tcaccgtatc
      421 gccgatgcgg atgtccttgc gggcgatctc gtcggcgttg tgcagggtgg cgttgcgcac
      481 gaccacgccg ccgaccgtca ccggatgcag ccgggcgacc ggcgtcaggg agccggtccg
      541 gccgacctgg atgtcgatgt cttccagcac ggtggtcgcc tgctgggccg ggaacttgtg
      601 cgcgatggcc cagcgcggcg agcgggagac gaaacccagc cgctgctgcc agtccagccg
      661 gtcgaccttg tagaccacgc cgtcgatgtc ataggccagc gtcggccggg ccgcgccgat
      721 gtcgcgatag acgccgagca ggccgtcggc gttctcgacc cgccgcgagc ggtcgttggt
      781 cgcgaaaccc cagtccgcca gacgctcgag agccccgctc tgggtatcgg cgaacggcgc
      841 gctgacctcg ccccatgcgt aggcgaagaa gcgcagcggc cgtctggccg tcaccgccgg
      901 gtcgatctgg cgcagcgacc cggccgcgaa gttgcgcgga ttggcgtagg tgcgggttcc
      961 ggcctcctcg gccgccctgt tgaacgcctc gaattcggcg aggggggcgt agacctcgcc
     1021 gcgaacctcg atgacatcgg gaacctggcc cttcagccgc tcgggaatgt cgtccagggt
     1081 gcgcagattg gcggtgatat cctcgcccgt gcggccatcg ccgcgcgtcg cgccgcgcac
     1141 cagcacgcct ttctcgtagc ggatcgaggc ggacaggccg tcgatcttcg gctcggccgt
     1201 gaaggccacc ggctcgtccg ccgccagccg caggaatcgg cgcacccggg cgacgaagtc
     1261 ctcgacctcc tcgtccgaga aggcgttgtc gaggctcagc atcggcacgc cgtgttcgac
     1321 gggcgcgaac tgctccgacc gcgcggcgcc gacccgcagg gagggactgt tgtcgcggat
     1381 cagatgcggg aagcgcgcct cgatggcctc gttgcgccgc ttgagggcgt cgtagtcggc
     1441 gtcgctgatc gtcggcgcgt cttcctgatg atagcgcagg tcatgggccg ccagctcgtc
     1501 ggccagccgc tcgagctcct cggccgcttc ggattcggtc aggtcgggga cgggggtgag
     1561 ggtcatgttg cttgcagcgt tgaacagttt gcgtggttcg acacgcgcct tcggcgctgc
     1621 tcaccaagac gcacacagat acacgtcatc ctgagcaggc cgcgcagcgg ccgtgtcgaa
     1681 ggacgcacgc cgctcacgcc ccgatcaacg cccgggccgc cgcccgcgcc gcgtcggtga
     1741 tctcggctcc ggcgagcatg cgggcgatct cctcctcgcg ggcggcgggc gacagggtct
     1801 ccaccgccgt gcggatgcgg gtctcgtcgc cgcccttgct gatgcgccag tgggcgtggg
     1861 cgcgggcggc gacctgcggg ctgtgggtca ccacc
//
LOCUS       DAORNX010000541         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44513_length_1895_cov_6.224457, whole genome shotgun sequence.
ACCESSION   DAORNX010000541 DAORNX010000000
VERSION     DAORNX010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44513_length_1895_cov_6.224457"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PLO65_16390"
     CDS             <1..159
                     /locus_tag="PLO65_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD29879.1"
                     /translation="IGKRVAQIQLEEAYRQILARFPDMRWTGEIDIAPNNFVHAIRKL
                     GVSFTPER"
     gene            235..1122
                     /locus_tag="PLO65_16395"
     CDS             235..1122
                     /locus_tag="PLO65_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRD29880.1"
                     /translation="MASPTFETILYDVEDGIATITMNRPDRMNAFTPQMMLDMIAAFD
                     ATDADDDVKVVIVTGAGDRAFCAGADLGSGGSTFDYGKQDNPTREGAKVNGVYRDGGG
                     RLTLRIYDSLKPVIGAVNGAAVGIGVTMQLPMDIRMASTSARFGFVFARRGITPEAAS
                     SWFLPRLVGMQQALEWCFTGRVFGAQEALDGGLVRSIHAPEDLLPAARALAREIADNT
                     APVSIALTRQMLWRMAGADQPMEAHKADSRAIMARGAQGDAREGVTSFLEKRPPAYPD
                     KVSADLPDIWPYWDKREFK"
     gene            1259..1729
                     /locus_tag="PLO65_16400"
     CDS             1259..1729
                     /locus_tag="PLO65_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD29881.1"
                     /translation="MTLALLLLFEVYQAFFWLDPSPVFGLIAARSGISLFEPGLRYLT
                     GVLELAAAILLVIPRSRSWGARLAAVVVLGAIGFHLSPWLGIQIPDPEIVSRALALGH
                     TAEAIDANHLPTDHGAMFILALAILALSVLTLVTERALARASAARPRRVMGAFA"
BASE COUNT          276 a          700 c          620 g          299 t
ORIGIN      
        1 atcggcaagc gcgtggcgca gatccagctg gaggaggcct accgccagat cctcgcccgc
       61 ttccccgaca tgcgctggac cggcgagatc gacatcgcgc cgaacaactt cgtccacgcc
      121 atccgcaagc tcggcgtcag cttcacgccg gagcgctagg gcggcgggcg gcccccttgc
      181 cttccgccac gtccgtccgt cttcatccgt ccgaccgaac ttccaggagc ccccatggcc
      241 agccccacct tcgagaccat cctctatgac gtcgaggacg gcatcgcgac gatcaccatg
      301 aaccggcccg accggatgaa cgccttcacg ccgcagatga tgctggacat gatcgcggcc
      361 ttcgacgcca ccgacgcgga cgacgacgtc aaggtggtca tcgtcacggg cgcgggcgac
      421 cgggccttct gcgccggggc ggacctgggc agcggcggct cgaccttcga ctacggcaag
      481 caggacaacc cgacccgcga gggggccaag gtcaacggcg tctaccgtga cggcggcggt
      541 cgcctgaccc tgcggatcta cgacagcctc aagccggtga tcggcgcggt caacggcgcg
      601 gcggtcggca tcggcgtgac catgcagctg ccgatggaca tccgcatggc ctcgaccagc
      661 gcccggttcg gcttcgtctt cgcccgccgc ggcatcaccc ccgaggcggc ctcgtcctgg
      721 ttcctgccgc ggctggtcgg catgcagcag gcgctggaat ggtgcttcac cggccgcgtg
      781 ttcggcgccc aggaggccct cgacggcggg ctggtccgct cgatccacgc gcccgaggac
      841 ctgctgccgg ccgcccgcgc cctggcccgc gagatcgccg acaacaccgc gccggtatcg
      901 atcgccctga cccgccagat gctctggcgc atggccggcg cggaccagcc gatggaggcg
      961 cacaaggccg acagccgggc catcatggcc cgcggcgccc agggcgatgc gagggaaggg
     1021 gtcacctcgt tcctcgagaa acgcccgccg gcctatccgg acaaggtttc cgccgacctg
     1081 ccggacatct ggccctactg ggacaagcgc gaattcaagt agtcggccgg gcattccgcg
     1141 ctcgaaaccg tgaatcgcct tagtcggcga gcggcgccat ggtgatggcc acagggacag
     1201 caacagtccc ggtggaggat tcgtcatgat ggagcgcgta cttagctgga cgttcggctt
     1261 gaccctcgcc cttcttcttc tgttcgaggt ctatcaggcc ttcttctggc tcgaccccag
     1321 tcccgtcttc ggcctgatcg cggcccgcag cggcatcagc ctgttcgagc cgggactccg
     1381 atatctgacc ggtgtgctgg aactggccgc ggccatcctg ctggtcatac cgcgcagccg
     1441 cagctggggc gcgcggctgg ccgccgtggt ggttctgggc gcgatcggct ttcacctgtc
     1501 gccctggctg ggcatccaga ttccggaccc ggaaatcgtc tcccgggccc tggcgctcgg
     1561 tcatacggcc gaggcgatcg acgccaatca cctgcccacc gatcacggcg ccatgttcat
     1621 cctcgccctg gccatcctgg cgctgtcggt gctgaccctc gtcaccgaac gcgccctggc
     1681 gcgagcgtcg gcggcaaggc cccgacgggt gatgggcgcg ttcgcgtgac ggtcacggcc
     1741 ggttaacagc cgtctgttgt cattggggcg cggcgcgttc gggcgtcgcc cgcctccgcg
     1801 ccccgtgatc catgagcgaa ttcgccgcca ggcccgtcct cgacccgccc gacttcggtc
     1861 aggcgcggga ccctgcgccg gcccccacgc cggcg
//
LOCUS       DAORNX010000542         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44555_length_1894_cov_9.166939, whole genome shotgun sequence.
ACCESSION   DAORNX010000542 DAORNX010000000
VERSION     DAORNX010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44555_length_1894_cov_9.166939"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLO65_16405"
     CDS             <1..398
                     /locus_tag="PLO65_16405"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRD29882.1"
                     /translation="PKRLRYPLEHDRAIETLALRIFLSVVEQALCRACPAAGSDSRIG
                     AVAFIHRFGALLHPHEHFHCLVIEGVFEADASGVATFHESRVPDQKWLDEVHAKVRRR
                     LLHALTRRGVLEPEDAETIANLGSLLPLR"
     gene            complement(1414..1894)
                     /locus_tag="PLO65_16410"
                     /pseudo
     CDS             complement(1414..1894)
                     /locus_tag="PLO65_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          310 a          611 c          636 g          337 t
ORIGIN      
        1 ttcccaagcg gttgcgctat cccctcgaac atgatcgggc gatcgagacg ctggcgctgc
       61 gcattttcct gagcgtggtg gaacaagcct tgtgccgcgc ctgcccggcg gcgggttccg
      121 attcgcggat cggcgcggtg gccttcatcc accgctttgg cgcgctgctc catccgcatg
      181 agcacttcca ctgcctcgtc atcgagggcg tcttcgaggc ggacgcttca ggagtagcga
      241 cgtttcacga aagccgtgtc cccgaccaga agtggctcga cgaggtccac gccaaggtcc
      301 gccgccgtct gctgcacgcg ctgacccgac gcggtgtgct tgagccggaa gatgccgaga
      361 cgatagcgaa cttggggtcg ctgctgccct tgaggtaaag gttgccgccc agcttgccct
      421 ccaccggatc ggcctcggcg aaggcagtcg tcttccaggt ccaggcgggg ccgagcaggt
      481 cgagggcggc gtaggtggtc accagcttca tgaccgaggc gggattcatc gcctggcggg
      541 cgttgtggga caccagggtg aagtgggaat caacggaatg aaccaccacc gccacactgt
      601 tcgcgggaat ctggacggac ttgagggcgc tgaggacagg cgggggaagc ccttcggcgg
      661 cgacaacggc ggggccgcac agcaggaaaa caaagggggg aggcgccgga aggggggcgt
      721 ggcgaagttg ggggcgcgag tggaaagtcc attgtggcgc ggcttacggt gagtcgataa
      781 tgcgggcatt ctacggaagg accgccgcca tggacacctc gtcccttgcc cgcgccgctc
      841 gcctgatcgc gaccgccgat gcgctcttca tcactgccgg cgccggcatg ggagtggatt
      901 ccggccttcc tgacttccgt ggccggcagg gcttctggcg ggcctacccg gcggtcgccc
      961 gggcgcggat cgatttcgag gaggtggcct cgcccgcaag cttccgccgc gacccggtgc
     1021 tggcctgggg attctacggc caccgcctgg ccacagcctg tcgtgagttc gccgccctcg
     1081 cctcgttcag ggggtgccaa acgccaatct gtggggtggc aggtagcgac gccagcgccg
     1141 aatatcaaag tacatcgccg gcccgctgga tgacacctcc agcccgccgg ccgaacatcg
     1201 gcaggaggtc ggcatggcca gcattcagtc accagcgggc acctaggacg cttatgtcct
     1261 gtggtttgct tccctgttca acccgggccg cggcgtccag gtgccttgtg atcagtcagg
     1321 gcatgtggcg cttgacgacc tgccggacag gctcaggaac gcctacttct gcgccagggc
     1381 tctggtaggc caatggcact acctttattc gaccagcgcc tgctgagcct cggcgcgcgt
     1441 gcggaaatgt tctccatgca cgcactcgac cttgaccgtg ccgttggccg attccatcgg
     1501 cgcgttgtcc cagcagtcgc ccttgcggct cattgaaact gtaattccgc gcgcctggag
     1561 aaccttgcga tagtcgctgg cggcgtattg cgacccgcga tccgagtgat gcatcaggcc
     1621 cggcgccggg ttccgccagc ccaaggccac ggccagcgct gccagcgtca gctcttgcgg
     1681 caccgtcgca ctcatcgccc agccaaccag cttgcgtgag aacagatcaa gcagcagtgc
     1741 caggtacaac cagccctcgt cggtcgggat gtaggtcagg tccgccagcc acttctggtt
     1801 cggcgcggtg gcctcgaaat ctcgtccgag aacgtttgga gcgaccggat ggagatgctt
     1861 cgagtccgtg gtcaccacgc ggaaccggcg ccgt
//
LOCUS       DAORNX010000543         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44871_length_1884_cov_6.108803, whole genome shotgun sequence.
ACCESSION   DAORNX010000543 DAORNX010000000
VERSION     DAORNX010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44871_length_1884_cov_6.108803"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLO65_16415"
     CDS             <1..232
                     /locus_tag="PLO65_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRD29883.1"
                     /translation="GAWRVSLGAGLFGALASAGWFVALALSPAGPVRAVGVVEMPMAA
                     LAGRRLFAERVTLRQWLAGGVTAFGVVLAAFG"
     gene            407..1450
                     /locus_tag="PLO65_16420"
     CDS             407..1450
                     /locus_tag="PLO65_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRD29884.1"
                     /translation="MFGSLFGAISNDIAIDLGTANTLIYMKGKGIVLNEPSVVALRNV
                     GGRKVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKGF
                     VNPKVIVCVPSGATAVERRAINDSCLNASARRVGLIDEPMAAAIGAGLPIHEPTGSMV
                     VDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKE
                     IGTARAPADGEGLSIDVKGRDLMQGVPREVRISEKQAADALQDPVNQIVEAVKVALEA
                     TPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH
                     PKWMKGVLESTLS"
     gene            1498..>1884
                     /locus_tag="PLO65_16425"
     CDS             1498..>1884
                     /locus_tag="PLO65_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRD29885.1"
                     /translation="MALRQDSFGELKVPLTWMAVVALIVCGVVGVSILLSDRRETFQT
                     EAYGRARQAVDTVSAPVGGAVAAPGRWTGDLLGWVRGYIAVGHENRRLRRVMVDLQRE
                     ADEAIRLRDENERLRAMLGVRTDPPVP"
BASE COUNT          278 a          623 c          675 g          308 t
ORIGIN      
        1 cggggcctgg cgggtttccc ttggcgcggg cctgttcggc gccctcgcct cggccggctg
       61 gttcgtcgcc ctggcgctgt cgccggcggg gcccgtccgg gcggtcggag tggtcgagat
      121 gccgatggcc gccctcgccg gacggcggct gttcgccgaa cgggttacgt tgcgccagtg
      181 gctggccggg ggcgtcacgg ccttcggcgt cgtcctcgcc gccttcggtt aggacgccgc
      241 cttaatagac cttgaattca acacgttcgc agtgcacacg gcgtgatgtc agcttgccct
      301 gccatctcgg cgacgctgcg ctccgggggg gcgcgctcgc ggcttggtgc ggggtttgca
      361 ggctcagcct cgcccgtccc aaaagctctc agattcaagg ctctgaatgt tcggctctct
      421 cttcggcgcc atttccaatg acatcgccat cgatctcggc acggccaaca ccctgatcta
      481 catgaagggc aagggcatcg tgctgaacga gccgagcgtc gtggcgcttc gcaacgtcgg
      541 cggccgcaag gtggtgcatg cggtcggcat cgaggccaag cagatgcttg gccgcacccc
      601 cggccacatg gaagccatcc gtccgatgcg tgacggggtc atcgccgact tcgaagtcgc
      661 cgaggagatg atcaagtact tcatccgcaa ggttcacaac cgcaagggct tcgtgaaccc
      721 caaggtgatc gtctgcgtgc cgtcgggcgc gaccgccgtg gaacgccggg cgatcaacga
      781 ttcctgcctc aacgcctcgg cccgccgggt cggcctgatc gacgagccga tggccgcggc
      841 gatcggcgcc ggcctgccga tccacgagcc gaccggctcg atggtcgtcg acatcggcgg
      901 cggcaccacc gaggtggccg tgctgtccct gtcgggcatc gtctattcgc gcagcgtgcg
      961 cgtcggcggc gacaagatgg acgaggcgat catcagctac atgcgccgcc accataacct
     1021 gctgatcggc gagaccacgg ccgagcggat caagaaggag atcggcacgg cgcgcgcgcc
     1081 ggccgacggc gaggggctgt cgatcgacgt caagggccgc gacctgatgc agggcgtgcc
     1141 gcgcgaggtg cgcatctccg agaagcaggc ggccgacgcg ctgcaggacc cggtcaacca
     1201 gatcgtcgag gcggtcaagg tggcgctcga ggcgacgcct cccgaactgg ccagcgatat
     1261 cgccgacaag ggcatcatgc tgaccggcgg cggcgcgctg ctgcggggcc tcgacgccga
     1321 gatccgcgac cataccggcc tgccggtgac ggttgccgac gatccgctgt cctgcgtggc
     1381 cctcgggtgt ggcaaggttc ttgaacaccc gaagtggatg aagggcgtac tggaaagcac
     1441 gctctcttaa catcagcatg tgagtcgggc cgggggcggc cctgacggag gcattgggtg
     1501 gccctgcgcc aggattcgtt cggggaattg aaggtcccgc tgacctggat ggcggtcgtg
     1561 gccttgatcg tctgcggggt cgtgggcgtc tcgattctgc tcagcgaccg tcgcgaaacg
     1621 ttccagaccg aggcctatgg ccgtgcgcgc caggcggtgg ataccgtttc cgccccggtc
     1681 ggcggcgccg tggccgcgcc cggccgctgg acaggcgacc tgctgggctg ggtccgcggc
     1741 tacatcgccg tggggcacga gaaccggcgc ctgcgccgcg tgatggtcga tctgcagcgc
     1801 gaggccgacg aggccatccg gctgcgtgac gagaacgagc gcctgcgggc gatgctcggc
     1861 gtgcgcaccg acccgccggt gccg
//
LOCUS       DAORNX010000544         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_44893_length_1883_cov_6.410832, whole genome shotgun sequence.
ACCESSION   DAORNX010000544 DAORNX010000000
VERSION     DAORNX010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44893_length_1883_cov_6.410832"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLO65_16430"
     CDS             <1..288
                     /locus_tag="PLO65_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29886.1"
                     /translation="EPLGLPQRTRDGVLVGVLSHATALASRNLRRARGMKGPAVWWAE
                     DPHDPPRLYADILRLDPVGPDWRPPEGFTPLERVHLPPEEMAEAFWRTRMR"
     gene            complement(448..>1883)
                     /locus_tag="PLO65_16435"
     CDS             complement(448..>1883)
                     /locus_tag="PLO65_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRD29887.1"
                     /translation="NQSTCNQDYSCVDGFCPSFITLEGAENATREKMAALTAEATPLP
                     EFEPLEGVRRILFTGIGGTGVTTVASILAMAAHIDGRAASVVDMTGLAQKGGSVFSHV
                     KIGEREDTVVGGRVPAASTDVLIACDLLVAASPEGLALFSKKTTRAFGNADFSPTADF
                     VTNRDVRFDTAGMARKVSAATKSYEACPSQALAEHKLGDGIYANMIMLGFTWQKGVIP
                     ISSRSIYRAIKLNGVDYESNLQAFEVGRKAAVDPSFRGEPEDAVATPETMPLDELIAR
                     RTAELTAYQNATWADRYAKRVAQVRAAEAPLGGEALARAVAVNLYKLMAYKDEYEVAR
                     LYTDGRFASELAGTFRGGKVKVWMSPPLIAPKDADGKPKKIALGGWMIDLVFPLLTRL
                     KGLRGGPLDIFGMTEERKMERGLIVAYEAQLDMILAGLTAERLALAARIAAVPQQIRG
                     YGHIKEASVKVAKADEAALWAEWKTEG"
BASE COUNT          248 a          668 c          665 g          302 t
ORIGIN      
        1 gaaccgctgg gccttccgca gcgcacgcgc gacggggtgc tggtcggggt gctcagccac
       61 gccaccgccc tggccagccg caacctgcgg cgggcgcggg ggatgaaggg gccggcggtc
      121 tggtgggccg aagacccgca cgacccgccc cggctctacg ccgacatcct gcggctcgac
      181 ccggtcggtc cggactggcg gccgccggaa gggttcacgc cgctggagcg ggtccatctg
      241 ccgccggagg agatggccga ggcgttctgg cgcacgcgga tgcggtgaac gtcgatccga
      301 tcctccccga cttcgcgggg aggaggggcg gcgctggtgc gagccgccac gtcctgaatt
      361 ccggtccctc cggccaggct ggcgctgccc atccaccatg cttcgcatgg tccccctccc
      421 cgacttcgcg gggaggaatt ccgcgctcta cccctccgtc ttccactccg cccagagcgc
      481 cgcctcgtcg gccttggcga ccttcaccga cgcttccttg atgtggccgt agccgcggat
      541 ctgctgcggg accgcggcga tgcgcgcggc cagggccagg cgctccgccg tcaggccggc
      601 gaggatcatg tccagctggg cctcgtaggc gacgatcagc ccgcgctcca tcttccgctc
      661 ttcagtcatg ccgaagatgt ccagcgggcc gccgcgcagg cccttcagcc gcgtcaacag
      721 cgggaacacc aggtcgatca tccagccgcc gagggcgatc ttcttcggct tgccgtcggc
      781 gtccttcggc gcgatcagcg gcgggctcat ccacaccttg accttgccgc ccctgaacgt
      841 tccggccagc tccgacgcga accggccgtc ggtgtacagc cgcgcgacct cgtactcatc
      901 cttgtaggcc atcagcttgt agaggttgac ggccaccgcc cgggccaggg cctcgccgcc
      961 cagcggcgcc tcggccgccc gcacctgggc cacgcgcttc gcatagcggt cggcccaggt
     1021 ggcgttctgg taggccgtca gctcggccgt ccgccgggcg atcagctcgt cgagcggcat
     1081 cgtctcgggc gtggcgaccg cgtcctcggg ctcgccacgg aagctcggat cgacggcggc
     1141 cttgcggccg acctcgaagg cctggaggtt ggactcgtag tcgacgccgt tgagcttgat
     1201 cgcgcgatag atcgagcggc tcgagatcgg gatcacgccc ttctgccagg tgaagcccag
     1261 catgatcatg ttggcgtaga tgccgtcacc cagcttgtgc tcggccaggg cctgggacgg
     1321 gcaggcctcg tagcttttcg tggccgcgct gaccttgcgg gccatgccgg cggtgtcgaa
     1381 gcgcacgtcg cgattggtca cgaagtcggc cgtcggcgag aagtcggcgt tgccgaaggc
     1441 gcgggtggtc ttcttcgaga acagggccag gccctcgggg ctggcggcga ccagcaggtc
     1501 gcaggcgatc agcacgtcgg tcgaggcggc cggcacgcgg ccgccgacca cggtgtcctc
     1561 acgctcgccg atcttgacgt ggctgaacac cgagccgccc ttctgggcca ggccggtcat
     1621 gtcgaccacg ctggcggcgc ggccgtcgat gtgggcggcc atggccagga tcgaggccac
     1681 ggtcgtcacg cccgtgccgc cgatgccggt gaacaggatg cggcgcaccc cctccagcgg
     1741 ctcgaactcg ggcagcggcg tggcctcggc ggtcagggcg gccatctttt cgcgggtggc
     1801 gttctcggcc ccctcgaggg tgatgaacga cgggcagaag ccgtcgacgc agctgtagtc
     1861 ctggttgcag gtcgactggt tga
//
LOCUS       DAORNX010000545         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_45294_length_1869_cov_9.720507, whole genome shotgun sequence.
ACCESSION   DAORNX010000545 DAORNX010000000
VERSION     DAORNX010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45294_length_1869_cov_9.720507"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PLO65_16440"
     CDS             <1..1481
                     /locus_tag="PLO65_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRD29888.1"
                     /translation="TPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQVAEQSFFE
                     SSNDGTVRIVGPTVKASNGEFVVMSRNTTITVQVDGKDREAYKPPYGARLKAKDGDKV
                     KRGDRLAEWDPYTAPIITEVAGKIRAEDLVDGFSVREETDEATGIANRVITDWRTSTR
                     GSDLRPALAVIDADGAYKRLSNGGEARYLLPVGAVLSVADGDEVKPGEVIARMPTEGA
                     KTRDITGGLPRVAELFEARRPKDCAVIAEMDGRVEFGRDYKNKRRIKITPEDGGEAVE
                     FLIPKGKHITVHDGDVILRGEYIIDGNPDPHDILRILGVEALANFLVDEIQEVYRLQG
                     VPINDKHIEVIVRQMLQKVEILEPGDTGLIKGDHLDKPDLDAENAKAVARGGRPAVVQ
                     PVLLGITKASLQTRSFISAASFQETTRVLTDASVHGKVDTLEGLKENVIVGRLIPAGT
                     GSYLRGLQRIATKRDEALSASREEAMEPLPVEIALDAETAEA"
     gene            complement(1545..>1869)
                     /locus_tag="PLO65_16445"
     CDS             complement(1545..>1869)
                     /locus_tag="PLO65_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29889.1"
                     /translation="HPAPLPAPSRGDLRQALRGSAVGCANRASVGLTRREREICDEAY
                     GRGGASETFIEPPMDPAKRAAFDAAAARKATARRRKESPPPPGINPTDNAGGTRSNGI
                     GILGY"
BASE COUNT          314 a          637 c          648 g          270 t
ORIGIN      
        1 gcacgccggt gaacatcggt gaagccgtgg gcgtcatcgc cgcccagtcg atcggtgagc
       61 cgggcaccca gctgaccatg cggaccttcc acatcggcgg cgccgcgcag gtggccgagc
      121 aatcgttctt cgaaagcagc aacgacggca cggtgcggat cgtcggtccg acggtgaagg
      181 cctccaacgg cgaattcgtc gtcatgagcc gcaacaccac catcaccgtc caggtcgatg
      241 gcaaggaccg cgaggcgtac aagccgccgt acggcgcccg cctgaaggcc aaggacggcg
      301 acaaggtcaa gcgcggcgat cgcctcgccg agtgggaccc ctacaccgcc ccgatcatca
      361 ccgaggtcgc cggcaagatt cgcgccgagg acctggtgga cggcttctcc gtccgcgagg
      421 aaaccgacga ggccacgggt atcgccaacc gcgtgatcac cgactggcgg acctcgaccc
      481 gcggttcgga cctgcgtccg gccctggcgg tgatcgacgc cgacggcgcc tacaagcgcc
      541 tgtcgaatgg cggcgaggcc cgctaccttc tgccggtcgg cgcggttctg tcggtcgctg
      601 acggcgacga ggtcaagccc ggcgaggtca tcgcccggat gccgaccgaa ggcgccaaga
      661 cgcgggacat caccggcggt ctgccgcggg tggccgagct gttcgaggcc cgtcgtccga
      721 aggactgcgc ggttatcgcc gagatggacg gccgcgttga attcggccgc gactacaaga
      781 acaagcgccg catcaagatc accccggaag acggtggcga ggcggtcgag ttcctgatcc
      841 cgaagggcaa gcacatcacc gtccacgacg gcgacgtgat cctgcgcggc gagtacatca
      901 tcgacggcaa ccccgatccg cacgacatcc tgcgcatcct gggcgtcgag gcgctggcca
      961 acttcctcgt cgacgagatc caggaggtct atcgtctgca gggcgtgccg atcaacgaca
     1021 agcacatcga ggtgattgtc cggcagatgc tgcagaaggt ggaaatcctc gagccgggtg
     1081 acaccggcct gatcaagggc gatcatctcg acaagcccga cctggatgcg gaaaacgcca
     1141 aggcggtcgc ccggggcggt cgtccggcgg tcgtccagcc cgtgctgctc ggcatcacca
     1201 aggcgtcgct gcagacccgc agcttcatct cggcggcctc gttccaggag accacgcgcg
     1261 tcctcaccga cgcctcggtc catggcaagg tcgatacgct cgagggcctc aaggagaacg
     1321 tcattgtcgg ccggctgatt ccggcgggca cgggctccta cctgcgcggc ctgcagcgca
     1381 tcgccaccaa gcgcgacgag gccctctccg cctcgcgcga ggaggccatg gagccgctgc
     1441 cggtcgagat cgcgctggac gcggagaccg ccgaggcctg accgacctcg gagactgaag
     1501 atcgagacgg cccgggcgca atcccgggcc gttttcgttt gcggtcagta ccccaggatg
     1561 ccgatgccgt tggagcgcgt ccctccagcg ttgtcggtcg gattgatccc cgggggcggc
     1621 ggcgattcct tgcgccggcg ggctgtcgcc ttgcgggccg cggccgcgtc gaaggcggcc
     1681 cgtttggcgg ggtccatggg cggttcgatg aaggtctctg aggccccgcc acgtccgtag
     1741 gcctcgtcgc agatctcgcg ttcccggcgc gtcaatccga cgctggcgcg gttggcgcag
     1801 ccgaccgcgg agccgcgcag ggcctggcgc agatcacctc gcgacggggc gggcaggggc
     1861 gcggggtgt
//
LOCUS       DAORNX010000546         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_45785_length_1852_cov_9.858653, whole genome shotgun sequence.
ACCESSION   DAORNX010000546 DAORNX010000000
VERSION     DAORNX010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45785_length_1852_cov_9.858653"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="argE"
                     /locus_tag="PLO65_16450"
     CDS             complement(<1..884)
                     /gene="argE"
                     /locus_tag="PLO65_16450"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRD29890.1"
                     /translation="MISSTAALADDAIAILRTLVGFDTTSRSSNLALIAWVEAYLTAQ
                     GVASRRIASADGDKANLLATVGPAVAGGVILSGHTDVVPVDGQPWTSDPFVLTERDGK
                     LFGRGSADMKGFIALALAAVPLFREGLKRPVHLAFSYDEEVGCFGAPEMIATIARDLP
                     RPALAIVGEPSLMKAVGGHKGAAFFRVLVSGREAHSSQPHLGVSANMAAVELLATLSA
                     LAADLAAKADPASPFEPKGTSLTVGRIDGGTAANILARRCEITFDVRAEPGDDPLVVL
                     APFVAQVADLDARLKARDP"
     gene            1032..>1852
                     /gene="purU"
                     /locus_tag="PLO65_16455"
     CDS             1032..>1852
                     /gene="purU"
                     /locus_tag="PLO65_16455"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRD29891.1"
                     /translation="MILTIACPDRRGIVAAVSAFLAQTGCNILDAQQFDDAETDVFFM
                     RVVFRPDDLAADDLREAFAPLAEQFAMRWSLRDPAVRKRVMILASKSDHCLADLLYRW
                     RIGELPMDIAAVVSNHPAEVYRHLDLSDLPFHHLPVTRETKLEQEAALWRLIGETRTD
                     LVVLARYMQVLSDGLAAKLEGRCINIHHSFLPGFKGAKPYHQAHARGVKVIGASAHFV
                     TGDLDEGPIIEQDVERISHRDTPEDLIRKGRDIERRVLARAVRWTLEDRVLLNGR"
BASE COUNT          292 a          656 c          634 g          270 t
ORIGIN      
        1 gggtcgcggg ccttcagccg ggcgtccagg tcggccacct gggccacgaa cggagccagc
       61 acgaccagcg ggtcgtcgcc cggctcagcg cgcacatcga aggtgatctc gcaccgtcgg
      121 gccaggatgt tggccgccgt accgccatcg atccggccga cggtcaggct ggtccccttt
      181 ggctcgaacg gcgaggccgg atccgccttc gcggcgaggt cggccgccag ggccgacagc
      241 gtcgccagca gctcgaccgc cgccatattg gccgagacgc cgaggtgcgg ctggctcgaa
      301 tgggcctcgc ggccgctgac cagcacgcgg aagaaggctg cgcccttgtg cccgccgacc
      361 gccttcatca gcgacggctc gccgacaatg gccagggccg ggcgcggcag gtcgcgggcg
      421 atcgtggcga tcatctccgg cgcgccgaag cagccgacct cctcgtcata ggagaaggcc
      481 aggtgcacgg gccgcttcag accctcgcga aacagcggga ccgcggccag ggccagggcg
      541 atgaagccct tcatgtcggc cgatccccgc ccgaagagtt tcccgtcgcg ctcggtcagc
      601 acgaaggggt cggaggtcca gggctggccg tcgacgggca cgacgtccgt atgccccgac
      661 aggatcaccc cgcccgccac cgccggtccg acggtggcca gcaggttggc cttgtcgccg
      721 tcggcgctgg cgatgcgccg gctggcgacg ccctgggccg tcagataggc ctcgacccag
      781 gcgatcaacg cgagatttga gcttcgggac gtcgtgtcga acccgaccag agtgcgcagg
      841 atggcgatgg cgtcgtcggc aagggccgcg gtggaggaga tcatggggga tgtgtagcgc
      901 gaggaacagg gacatgcact tcagtgcgtg tcccttgcag cgaaagggga cacgtactga
      961 agtacatgtc cccgcgccct tgccccgtaa gcttctcgcc cttaagcccc tcgtccatgt
     1021 cgacccaggg cctgatcctc accatcgcct gccccgatcg tcgcggcatc gtcgcggcgg
     1081 tttcggcctt cctggcccag accggctgca acatcctcga cgcgcagcag ttcgacgacg
     1141 ccgagacgga cgtcttcttc atgcgggtgg tgttccggcc cgacgacctg gccgccgatg
     1201 acctgcgcga ggccttcgcc ccgctggccg agcagttcgc catgcgctgg agcctgcgcg
     1261 acccggcggt gcgcaagcgg gtgatgatcc tggccagcaa gtccgaccac tgcctggccg
     1321 acctgctcta tcgctggcgg atcggcgagc tgccgatgga tatcgccgcc gtcgtctcca
     1381 accacccggc ggaggtctat cggcacctcg acctgtcgga cctgccgttc caccacctgc
     1441 cggtgacgcg cgagaccaag ctcgagcagg aggcggctct ctggcggctg atcggcgaga
     1501 cccggaccga cctggtggtg ctcgcccgct acatgcaggt gctgtccgac ggcctggcgg
     1561 ccaagctgga aggccgctgc atcaatatcc accactcgtt cctgccgggc ttcaagggcg
     1621 ccaaacccta tcaccaggcc catgcgcggg gcgtgaaggt gatcggcgcc agcgcccact
     1681 tcgtgaccgg cgacctcgac gagggcccga tcatcgagca ggacgtcgaa cgcatcagcc
     1741 accgcgacac gccggaagac ctgatccgca agggccgcga catcgagcgc cgcgtgctgg
     1801 cccgggccgt gcgctggacg ctggaggacc gggtgctgct gaacggccgc aa
//
LOCUS       DAORNX010000547         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_46169_length_1839_cov_6.767937, whole genome shotgun sequence.
ACCESSION   DAORNX010000547 DAORNX010000000
VERSION     DAORNX010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46169_length_1839_cov_6.767937"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLO65_16460"
     CDS             complement(<1..261)
                     /locus_tag="PLO65_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29892.1"
                     /translation="MLKRIEYGFPDILRERLAEGRPVEAIVDDWPSLALALERDPEAL
                     TAALREAAQLASDRGSANVAPRSAFGVAVVEAGGDLALADEVF"
     gene            441..1541
                     /locus_tag="PLO65_16465"
     CDS             441..1541
                     /locus_tag="PLO65_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRD29893.1"
                     /translation="MSAESLFQPFQLKSLTLKNRIVMAPMTRSFSPGGVPTDDVAAYY
                     ARRAANAVGLIVSEGTGVARPASLNDRNVPRFHGEPELAAWKKVINDVHVHGGLMAPQ
                     LWHVGAVRSRDPEWNPAGGYDSPSGLSSPGKSFGEPMSDEAVADAVDAFARAAGAAKT
                     LGFDSIELHGAHGYLIDQFFWDGVNLRTDRWGGATLPERSRFAAEILKAVRREVGEDY
                     PVIIRLSQWKQQDFTAKLAATPAEMEAWLQPLADAGADVFHCSQRRFWEPEFEGSDLN
                     FAGWAKKLTGKPTITVGSVGLSGEFIAAWGGEASQPASLDGLLERLDRGEFDMVAVGR
                     ALLQDPEWAVKVRDGRTDELQSFDRSAMAVLY"
     gene            1628..>1839
                     /locus_tag="PLO65_16470"
     CDS             1628..>1839
                     /locus_tag="PLO65_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29894.1"
                     /translation="MLTEIDRILLATPDAVGSAMPWRDLLGAEFVGRDRAGSLGAQRM
                     TLRVGTTDIEFLEPDGTGPVADALARR"
BASE COUNT          281 a          664 c          626 g          268 t
ORIGIN      
        1 gaagacttcg tcggccagcg ccaggtcgcc gcccgcctcg accaccgcca cgccaaacgc
       61 cgagcgcggg gccacgttgg ccgagccccg gtcggaggcc aattgcgccg cctcgcgcag
      121 cgccgcggtc agggcctccg gatcgcgttc cagcgccagc gccagcgacg gccagtcgtc
      181 gacgatcgcc tccacggggc gcccttcggc cagccgttcg cggagaatgt ccggaaagcc
      241 gtactcgata cgcttcaaca cgtcttggcc cccatccggg tccctcgacc ggatgtctga
      301 tcatcatacc gcgaaatggg ctggacaaga acaggactcc ccgaacacgg gcgccacatg
      361 gggtcgcgtg aagaaattct ccgtggcggg aggtgtcaaa cgggcgttta acaccccgtc
      421 atcccctccc ggagccgccc gtgtctgccg aaagcctctt ccagcccttc cagctcaagt
      481 ccctgacgct gaagaaccga atcgtcatgg cgcccatgac gcggtccttt tcgcccggcg
      541 gcgtgccgac cgacgatgtc gcggcctact atgcccggcg cgcggccaac gccgtcggcc
      601 tgatcgtgtc cgaggggacg ggggtggccc gccccgcctc gctcaatgac cgcaacgtgc
      661 cgcgcttcca cggcgagccc gaactcgcgg cctggaaaaa ggtgatcaac gacgtgcacg
      721 tccacggcgg cctgatggcg ccgcagctgt ggcacgtcgg cgcggtgcgt tcgcgcgatc
      781 ccgagtggaa cccggccggc ggctatgaca gtccgtcggg cctttccagc ccgggcaagt
      841 cgttcggcga gccgatgagc gacgaggccg tggccgacgc ggtcgacgcc ttcgcccggg
      901 ccgccggcgc ggccaagacc ctgggcttcg actcgatcga gctgcacggg gcccacggct
      961 atctgatcga ccagttcttc tgggacgggg tcaacctgcg caccgaccgc tggggcgggg
     1021 cgaccctgcc cgagcgctcg cgcttcgccg ccgagatcct caaggcggtc cgccgggagg
     1081 tcggcgagga ctatccggtg atcatccgcc tgtcgcagtg gaagcagcag gacttcaccg
     1141 cgaagctggc ggccacgccg gccgagatgg aagcctggct gcagccgctg gccgacgccg
     1201 gcgccgatgt gttccactgc tcgcagcgcc gcttctggga gcccgagttc gagggcagcg
     1261 acctgaactt cgccggctgg gccaagaagc tgaccggcaa gccgaccatc accgtcggtt
     1321 cggtcgggct gtcgggcgag ttcatcgcgg cctggggcgg cgaggccagc cagccggcct
     1381 ctctggacgg cctgctcgaa cgcctcgacc gcggtgaatt cgacatggtc gccgtcggcc
     1441 gggcgctgct gcaggatccc gaatgggcgg tcaaggttcg cgacggccgc accgatgagc
     1501 tgcagagctt cgaccgctcg gccatggccg tgctgtacta gacggacagg gggacatgcc
     1561 cttcagtgcg tgtccccatc cgcccgcaag ggacaggcac tgaagtgcat gtccctggac
     1621 accgcccatg ctgaccgaga tcgaccgcat cctgctggcc acacccgacg ccgtcgggtc
     1681 ggctatgccc tggcgcgacc tgctgggcgc cgagttcgtc ggccgcgacc gggccggcag
     1741 cctgggcgcc cagcgcatga cgctgcgcgt cgggaccacc gacatcgagt ttcttgagcc
     1801 ggacgggacg gggccggtcg ccgacgcgct cgcccggcg
//
LOCUS       DAORNX010000548         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_46676_length_1823_cov_7.042986, whole genome shotgun sequence.
ACCESSION   DAORNX010000548 DAORNX010000000
VERSION     DAORNX010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46676_length_1823_cov_7.042986"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLO65_16475"
     CDS             complement(<1..296)
                     /locus_tag="PLO65_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRD29895.1"
                     /translation="MDGAGADSPAFSVVATDPPPTPLVFASPHSGRHYPPEMLAAAAV
                     GAEALRRSEDAFVDEMIAPTGARGAALIMARHGRAWMDLNREPWELDPAMFDGDL"
     gene            595..954
                     /locus_tag="PLO65_16480"
     CDS             595..954
                     /locus_tag="PLO65_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD29896.1"
                     /translation="MPRILLAEDDDSLRNFLARALERAGYEVTACADGEEAAAVLDQD
                     WDLLLTDIVMPGMDGIEVARHAAARHPGLRIMFITGFAAVALTAGERAPAGAKVLSKP
                     VHLRDIVTEVERMMAAA"
     gene            complement(1157..1231)
                     /locus_tag="PLO65_16485"
     tRNA            complement(1157..1231)
                     /locus_tag="PLO65_16485"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1197..1199),aa:Val,seq:gac)
     gene            complement(1292..>1823)
                     /locus_tag="PLO65_16490"
     CDS             complement(1292..>1823)
                     /locus_tag="PLO65_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD29897.1"
                     /translation="GMQAVVLALRRLDKPSIAMIRGYAVAGGLALACACDFRLAAETA
                     RLGDTSGKVGLLPDEGGAWLFPCLMGYDKALKMTLLSEVYDAPTAAGLGLVTEVVADG
                     SLEERTLEFAAALATRAPLAVRLSKTMMVRAAGCSLEDSLIDAQHAVMIANPSADVRE
                     GIRAFREKRAPRFEGR"
BASE COUNT          272 a          663 c          620 g          268 t
ORIGIN      
        1 aggtcgccgt cgaacatcgc cgggtccagc tcccacggct cgcggttcag gtccatccag
       61 gcccggccat ggcgggccat gatcagggcc gcgccgcggg ccccggtcgg ggcgatcatc
      121 tcgtcgacga aggcgtcctc ggaccggcgc agggcctcgg ccccgacggc cgcggcggcc
      181 agcatctccg gcggatagtg gcggcccgaa tggggcgagg cgaacaccag cggcgtcggc
      241 ggcggatcgg tcgcgaccac gctgaaggcc ggactgtcgg cgcccgcgcc atccatcgtc
      301 ttcgccgccc gctgcatgtc catcccgggc atgatcgcgg cgcgatcggg gcgggtcaaa
      361 ggctttcggg gagtgggatt gaggttgcgt ggttcgagac gctcgcctga gggctcgctc
      421 ctcaccatga cgaacacctt tgcagcccac ccatatgcgt catcctgagg agccggcgca
      481 gccggcgtct cgaaggacgc acgttcccgc ccggcgcgag gttcatcgcg cctttacgcc
      541 tgacagggta gaatgccctc tgtcgcaaga tcatccgggg gccgttttcc gaccatgccg
      601 cgcattctcc tggccgaaga cgacgattcc ctgcgcaact tcctggcccg ggcgctggag
      661 cgcgccggct acgaggtgac cgcctgcgcc gacggcgagg aggccgccgc cgtgctcgac
      721 caggactggg acctgttgct gaccgacatc gtcatgcccg gcatggacgg catcgaggtg
      781 gcccggcacg ccgccgcccg gcacccgggc ctgcggatca tgttcatcac cggattcgcg
      841 gccgtggccc tgaccgccgg cgagcgcgcc ccggcgggcg ccaaggtgct cagcaagccc
      901 gtccacctgc gcgacatcgt caccgaggtc gaacggatga tggccgcagc gtagcggccg
      961 taccccgccg gggtcgtcct tgaccaggac gcccgaccgg agcgcatcat tccatcaagg
     1021 ccttcagggc tcttcatctt ccggcgggcc gaacggccca tcgtgatgcg cgagcggcat
     1081 cgacgcgctc gaacgccaaa tccgaaagga aatctgcgaa gtcgcccggg cccctggggc
     1141 cgatcccgtg aagggatggt ggacacggct gggattgaac cagcgacccc ctcgatgtca
     1201 acgaggtgct ctcccgctga gctacgcgtc cttgccaggg agggccgggt ctatagcggc
     1261 gcttcagcag gctggcaacc gcctcgcgcc gctatcgccc ttcaaatctc ggcgcccgct
     1321 tctcgcggaa ggccctgatc ccctcacgca cgtccgcgct cgggttggcg atcatcaccg
     1381 cgtgctgggc gtcgatcagc gagtcctcga ggctgcagcc cgccgcgcgg accatcatgg
     1441 tcttgctcag gcggacggcc agcggggcgc gagtggcgag ggcggccgcg aactccaggg
     1501 tgcgttcttc cagcgacccg tcggcgacga cttccgtcac cagccccagg cccgccgcgg
     1561 tcggggcgtc atagacctcg ctcagcaggg tcatcttcag cgccttgtcg tagcccatca
     1621 ggcaggggaa cagccaggcg ccgccctcgt ccgggaggag gccgaccttg cccgaggtgt
     1681 cgcccagcct cgccgtctcg gccgccagcc ggaagtcgca ggcgcaggcc agggccaggc
     1741 cgccggccac cgcgtagccg cggatcatgg cgatcgacgg cttgtccagc cgcctgagag
     1801 ccagaaccac cgcctgcatg ccc
//
LOCUS       DAORNX010000549         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_46681_length_1823_cov_4.026584, whole genome shotgun sequence.
ACCESSION   DAORNX010000549 DAORNX010000000
VERSION     DAORNX010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46681_length_1823_cov_4.026584"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLO65_16495"
     CDS             complement(<1..233)
                     /locus_tag="PLO65_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HRD29898.1"
                     /translation="MTAGPPSDAERVAWLRLARTPNVGPVAFEHLLQRCGSAIAAIEA
                     LPGLARRSVNIPSGTEAERELATGDRLGARLICG"
     gene            complement(230..880)
                     /gene="plsY"
                     /locus_tag="PLO65_16500"
     CDS             complement(230..880)
                     /gene="plsY"
                     /locus_tag="PLO65_16500"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRD29899.1"
                     /translation="METLAGAVWLTLGLIVAGGYLLGSIPFGMIATRLGGAGDIRKIG
                     SGNIGATNVLRSGRKDLAAITLVGDAGKGALAVLLAHLLTHGNAAAVALAGGSAFVGH
                     LFPVWLKFNGGKGVATFYGVLLSAAFPVGALAALTWIAMAFLFRISSLAALTAAALAP
                     LFAFAVDAPRPMIVLALVMAVLIFIRHHANIARLLKGEEPRIGAKKAEASTPTPGA"
     gene            complement(1051..>1435)
                     /locus_tag="PLO65_16505"
     CDS             complement(1051..>1435)
                     /locus_tag="PLO65_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD29900.1"
                     /translation="VDIVVSAHAPAPAEDKRLPYDEAAPGGVGLETLLPALLALYHDG
                     RAPLVDLLRAVTLAPARFLGLSSGRLAPGAPADLVLCDLGAPVIIDADRLVSKSKNSP
                     FDGRRLQGQVLRTVVAGKVVYRAAE"
     assembly_gap    1436..1554
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1555..1809)
                     /locus_tag="PLO65_16510"
     CDS             complement(<1555..1809)
                     /locus_tag="PLO65_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29901.1"
                     /translation="MAVAHRPADSWLTRGAAATEGEFAGRLGLPSAPAMAERIGLERD
                     LALAELTGALAGIPHLRRLRIHSRLPVVLPARVDAALLDWL"
BASE COUNT          260 a          605 c          620 g          219 t
ORIGIN      
        1 ccgcagatca accgcgcgcc caggcggtcg ccggtcgcga gctcgcgctc ggcctcggtc
       61 ccggacggaa tgttgacgga gcgtcgcgcg agaccgggca gggcctcgat ggccgcgatg
      121 gccgagccgc agcgctgcag caggtgttcg aaggccaccg gtccgacgtt cggggttctg
      181 gccaggcgca gccaggcgac ccgttcggcg tcggacggcg gcccggccgt cacgcgcccg
      241 gggtcggagt ggacgcctcc gccttcttcg cgccgatcct cggctcctcg cccttcagca
      301 accgggcgat gttcgcatgg tggcggatga agatcagcac ggccatgacc agcgccagca
      361 cgatcattgg ccggggggcg tcgaccgcga aagcgaacag gggcgccagc gccgccgccg
      421 tcagggccgc cagcgaggag atgcggaaca ggaaggccat ggcgatccag gtcagggccg
      481 ccagcgcccc gaccgggaag gcagccgaca gcagcacgcc gtagaaggtc gccacgccct
      541 tgccgccgtt gaacttcagc cagaccggga acaggtggcc gacgaaggcg ctgccgccgg
      601 ccagggccac ggcggcagcg ttgccgtggg tcagcagatg ggccagcagc acggccaggg
      661 cccccttgcc ggcgtccccg accagggtga tggcggccag atccttgcgg cccgagcgca
      721 gcacattggt tgcgccgatg ttgcccgaac cgatcttgcg gatgtccccc gcgccgccca
      781 gtcgggtggc gatcatgccg aacgggatcg agccgaggag gtagcccccc gcgacgatca
      841 ggcccagtgt cagccagacg gcgcccgcca aggtttccat agtcgcccct tatacacggt
      901 atggttgttg ttgtctcgtc acgtcgacgt tagagcgcgt cggcgccgga gtcatggggg
      961 tggcgccgct cggggacatg cgcttcagtg cgtgtcccct gccgctacga agccgatagg
     1021 gacacgtact gaagtacatg tccccgatcc ttactccgcc gcccggtaga ccaccttgcc
     1081 ggccaccacg gtccgcagca cctgaccttg cagcctgcgg ccatcgaacg gggagttctt
     1141 ggactttgac accagcctgt cggcgtcgat gatcaccggg gcgccgaggt cgcacaggac
     1201 caggtcggcc ggggcgccgg gggccaggcg gccgctgctc aggccgagga accgggccgg
     1261 cgcgagggtc accgcccgca gcaggtcaac cagcggcgcg cggccgtcgt gatacagggc
     1321 cagcagcgct ggcagcaggg tctccagacc caccccgccg ggcgccgcct cgtcataggg
     1381 caggcgcttg tcctcggccg gagccggggc gtgggccgag acgacgatgt ccacgnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagcca
     1561 gtccagcagc gccgcgtcca cccgcgccgg cagcacgacc ggcaggcggc tgtggatgcg
     1621 caggcgacgc aggtggggga tgcctgccag cgcgccggtc agttcggcca gggccagatc
     1681 gcgttccagc ccgatccgct cggccatcgc cggggccgag ggcaggccca ggcggccggc
     1741 gaattcgcct tccgtggccg ccgcgccccg ggtcagccag gagtcggccg gccggtgcgc
     1801 cacggccatg tcgaaagtcc tgg
//
LOCUS       DAORNX010000550         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_47054_length_1811_cov_4.025626, whole genome shotgun sequence.
ACCESSION   DAORNX010000550 DAORNX010000000
VERSION     DAORNX010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47054_length_1811_cov_4.025626"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLO65_16515"
     CDS             complement(<1..215)
                     /locus_tag="PLO65_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29902.1"
                     /translation="MSDEAPRGGVAPAAHADLALGSRVWFIAWLSSVGVTLLALALVY
                     PQIIWPVTPALAIGGLPAAVALATRRR"
     gene            420..1199
                     /locus_tag="PLO65_16520"
     CDS             420..1199
                     /locus_tag="PLO65_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD29903.1"
                     /translation="MPRPARPSVAASPVDRLDHPPAPSGRDSAADDAKRAFLRTVSHE
                     LRTPLNAIIGFSEILAAELYGPLGARQYREYAEHVRQSGYRLLKLVNQVLEIARLQGD
                     AIDLTAEVQPLDHVLDDVLDQLREDRRASGVRLVVEDEGRLPSVRADAKGVRTLLFNL
                     LQNALLHGAGGGLVEVSARRQGASVGIEIRDHGAGVDPADLPRLLAPFEQGDGALTRP
                     GRGAGLGLPIARLLAAAMGGQLLITSRPGDGLRAMVTLPAA"
     gene            1221..1655
                     /locus_tag="PLO65_16525"
     CDS             1221..1655
                     /locus_tag="PLO65_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRD29904.1"
                     /translation="MTSPIDAALEDLVAEFDLLGDWEEQLQYVIDLGREMPPLADTDR
                     SETNRVRGCASQVWLVTEQQPDGTLHFRGESDAIIVRGLIAVVLRLFDNRAPADILAF
                     DTPAAFARLGLSRMLSMQRSNGLASMVARIRRDAEAAQAPRP"
     gene            complement(1637..>1811)
                     /locus_tag="PLO65_16530"
     CDS             complement(1637..>1811)
                     /locus_tag="PLO65_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HRD29905.1"
                     /translation="AALAEVGPGLREILAHVCFAGTALEAAERTLGLPRRSGKTVLKL
                     ALGRLANHYGLGA"
BASE COUNT          255 a          665 c          623 g          268 t
ORIGIN      
        1 tggcggcggc gggtcgccag cgccacggcc gccggcagac cgccgatggc cagcgccggc
       61 gtcaccggcc agatgatctg cggatagacc agcgccaggg ccagcagcgt gacgccgacc
      121 gacgacagcc aggcgatgaa ccagacgcgg cttcccagcg cgagatccgc gtgcgccgcc
      181 ggagcgacgc cgcccctcgg ggcttcgtcg gacacgcttt cgcccactgg cttctcgtcc
      241 cctcacccca ttttggggcc tgacgcgaac actagcgacc catgcgccgg ggctccagac
      301 cgcacaatcc ccgggcgacg cgatttcgtg gacgctcgcc cctcaccctg tggatggaaa
      361 cggatttgta agcgcgatgc cccattatgg gcgcccgttt gtagtcgagg ccgtcggcca
      421 tgcccaggcc tgccaggcca tctgtcgccg cgtcacccgt cgatcggctg gaccacccgc
      481 ccgcgccgtc cgggcgcgac tccgccgcgg acgacgccaa gcgggccttt ctgcgcaccg
      541 taagccacga gctgcgcacc ccgttgaatg cgatcatcgg cttttccgag attctcgccg
      601 ccgagcttta cgggcctctg ggcgcccggc agtaccgcga atacgccgag cacgtgcgcc
      661 agagcggcta tcggctgctc aagctggtca accaggtgct tgagatcgcc cgcctgcagg
      721 gcgacgcgat cgacctgacc gccgaggtcc agccactgga ccacgtgctc gacgatgtgc
      781 tggaccagtt gcgggaagac cgccgcgcct cgggcgttcg cctcgtcgtc gaggacgagg
      841 gccgcctgcc gtcggtgcgg gccgacgcca agggcgtccg caccctgctg ttcaacctgc
      901 tgcagaacgc actgctgcac ggcgccggcg gcggccttgt cgaggtcagc gcccggcgac
      961 agggcgcgag cgtcgggatc gagatccgcg accatggcgc gggcgtcgac ccggcggacc
     1021 ttccgcggct tctggccccg ttcgaacagg gcgacggcgc cctgacgcgc ccggggcgcg
     1081 gggccgggct gggcctgccc atcgcgcggc ttctggccgc cgccatgggc gggcagttgc
     1141 tgatcacgtc gcgtcccggt gacggtctcc gggccatggt gaccttgccc gccgcctgat
     1201 ggggccttaa gtgcgcggcg atgacctccc cgatcgacgc cgccctcgaa gaccttgtcg
     1261 ccgaattcga cctgctcggc gactgggagg agcagctcca gtacgtcatc gatcttggtc
     1321 gcgagatgcc gccgctcgct gataccgacc gctcggaaac caacagggtt cgggggtgcg
     1381 cgagccaggt ctggctggtc accgagcaac agccggatgg cacgctgcat tttcgcgggg
     1441 aatctgacgc catcatcgtg cgcggcctga tcgccgtggt cctgcggctg ttcgacaatc
     1501 gcgcgccggc cgacatcctg gccttcgaca ccccggccgc cttcgcccgc ctggggctga
     1561 gccggatgct gtcgatgcag cggtccaacg gcctggcctc gatggtcgcc cgcatccgcc
     1621 gcgacgcgga ggccgctcag gcgcccaggc cgtagtggtt cgccagccgg ccgagggcga
     1681 gcttgagcac agtcttgcct gaccggcgcg gcaggccgag cgtgcgctcg gccgcctcga
     1741 gcgcggtgcc ggcgaaacag acgtgggcga gaatttcccg caggcccggc ccgacctcgg
     1801 ccagggccgc g
//
LOCUS       DAORNX010000551         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_47116_length_1809_cov_6.602052, whole genome shotgun sequence.
ACCESSION   DAORNX010000551 DAORNX010000000
VERSION     DAORNX010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47116_length_1809_cov_6.602052"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLO65_16535"
     CDS             <1..357
                     /locus_tag="PLO65_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HRD29906.1"
                     /translation="ETEAAGHPAVIRDRFARRLKDLAGEAASEERIVQEAAALAVKAD
                     VREELDRLAAHVASARGLLGEDVAAGRRLDFLTQEFMREANTLCSKSATAALTAIGLD
                     LKAVIEQFREQVQNVE"
     gene            347..1000
                     /gene="gmk"
                     /locus_tag="PLO65_16540"
     CDS             347..1000
                     /gene="gmk"
                     /locus_tag="PLO65_16540"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRD29907.1"
                     /translation="MSSEEPRKTRGLMLMIVAPSGVGKTSLTRRLVADHSDLHLSISA
                     TTRQPRPGEHEGRDYHFVSRADFDGMIEEDAFLEWAEVYGNRYGSPRAPIMDALARGE
                     SALFDIDYQGAMKIHAQAPDDSVLVYILPPSLAEMSRRLLARSQDSEEVIRERIARAK
                     DELVQWGTFDYVVLNDDFDRAYADLAHIFHAERRKASRNLWIAPFVDELLAEELPEL"
     gene            complement(1049..1807)
                     /gene="rsmA"
                     /locus_tag="PLO65_16545"
     CDS             complement(1049..1807)
                     /gene="rsmA"
                     /locus_tag="PLO65_16545"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRD29908.1"
                     /translation="MADKSFGQHFLLDLNITRKIARLAGPLEGQTVIEVGPGPGGLTR
                     ALLEAGAHVVAVEKDARFLPLLAELAQAADGRLTVVEGDALKVDEAALAGGPGHVVAN
                     LPYNVGTPLLIKWLTGPWRPVSMTLMFQKEVADRIAAAVNDDAYGRLAVICQALCRAR
                     VVMDLPARAFTPPPKVASAVVRLDPLADPPSPTEIAALERVSAAAFGQRRKMLRSSLK
                     PLGGEALCLKAGIDPGVRAETVSVEGFLSLARAL"
BASE COUNT          282 a          651 c          617 g          259 t
ORIGIN      
        1 gagaccgagg cggccggcca tccggccgtc atccgcgacc gcttcgcacg ccgtctgaag
       61 gatctcgccg gcgaggccgc gtcggaagag cgcatcgtcc aggaggccgc cgccctggcc
      121 gtcaaggccg acgtgcgcga ggagctggat cgcctcgccg cccatgtcgc ctccgcccgg
      181 gggctgctcg gcgaggacgt cgccgccggc cgccggctgg actttctgac gcaggagttc
      241 atgcgcgagg ccaacaccct gtgttcgaaa tctgcgacgg cggccttgac cgctatcggc
      301 ctcgacctca aagcggtcat cgagcagttc cgcgagcagg tgcagaatgt cgagtgagga
      361 accccgcaag acccggggtc tgatgctgat gatcgtggcg ccgtccgggg tgggcaagac
      421 ctcgctgacc cgccggctgg tcgccgacca ttcagacctg catctgtcga tctcggccac
      481 gacccgccag ccgcggcccg gcgagcacga gggccgcgac tatcatttcg tctcccgcgc
      541 ggacttcgac gggatgatcg aggaggatgc cttcctcgaa tgggccgagg tctacggcaa
      601 ccgctacggc agcccgcgcg cgccgatcat ggacgccctg gcgcggggtg agagcgccct
      661 gttcgacatc gactaccagg gggcgatgaa gatccatgcc caggcgccgg acgactcggt
      721 gctggtctac atcctgccgc cgtcgctggc cgagatgagc cgccgcctgc tggcccgctc
      781 gcaggacagc gaggaggtga tccgcgagcg gatcgcccgg gccaaggacg aactggtcca
      841 gtgggggacc ttcgactacg tcgtcctcaa cgacgatttc gaccgcgcct acgccgacct
      901 cgcccacatc ttccacgccg agcgccgcaa ggcctcgcgc aacctgtgga tcgcgccgtt
      961 cgtcgacgaa ctgctggccg aggaactgcc ggagctttga agtcgagatg cgtgcgggat
     1021 tcgggacgcc gctcaccatg acgtggaact aaagcgcccg cgccaggctc aggaacccct
     1081 ccaccgacac ggtttccgcc cggacgcccg ggtcgatgcc ggccttcagg cacagcgcct
     1141 cgccgcccag cggcttcagg ctggaccgca gcatcttgcg acgttggccg aaggcggcgg
     1201 ccgacaccct ctccagcgcc gcgatctcgg tgggcgacgg cgggtccgcc agcggatcaa
     1261 gccgcaccac cgccgaagcc acctttggcg gcggcgtgaa ggcgcgcgcc ggcaggtcca
     1321 tgacgaccct ggcccggcac agggcctggc agatcaccgc caggcggcca taggcgtcgt
     1381 cgttcaccgc ggcggcgatg cggtcggcga cttccttctg gaacatcagg gtcatcgaca
     1441 ctggccgcca gggcccggtc agccacttga tcagcaacgg cgtgcccacg ttgtacggca
     1501 ggttggccac gacatggccg gggccgccgg ccagggccgc ctcgtcgacc ttcagggcgt
     1561 caccctcgac cacggtcagc cggccgtcgg cggcctgcgc cagctccgcg agcagcggca
     1621 ggaagcgcgc gtctttctcg accgcgacca catgggctcc ggcctccagc agggcgcggg
     1681 tcaggccgcc ggggccgggg ccgacctcga tcaccgtctg gccctccagc ggtccggcca
     1741 gccgggcgat cttgcgcgtg atgttgaggt ccagcaggaa gtgctggcca aagctcttgt
     1801 cggccagca
//
LOCUS       DAORNX010000552         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_47536_length_1796_cov_6.422746, whole genome shotgun sequence.
ACCESSION   DAORNX010000552 DAORNX010000000
VERSION     DAORNX010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47536_length_1796_cov_6.422746"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLO65_16550"
     CDS             <1..197
                     /locus_tag="PLO65_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29909.1"
                     /translation="DSAGVQIGTVMDPDPTMHANGRVTIKTSTGFITVPRGSLTLEGG
                     VAVSRQTEADLRASGATKPQ"
     gene            327..716
                     /locus_tag="PLO65_16555"
     CDS             327..716
                     /locus_tag="PLO65_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HRD29910.1"
                     /translation="MAPARPNRRQGAGGTFLWDLLTFDRLMTGPVIHLIYWAGLGVII
                     IGFFGAIGAAVGVAMQDTNIIGRLLALPVVIAGLLVGFALGLLWRSFCEFYVAIFRIA
                     DDLRALRQASDADHVLKKPISPPSVDV"
     gene            complement(691..1755)
                     /locus_tag="PLO65_16560"
     CDS             complement(691..1755)
                     /locus_tag="PLO65_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRD29911.1"
                     /translation="MSPPSRRLVLASGLALAACGREPAAAQTVVDTPLKSLAPFPLGT
                     AVRAASLEEPAYADLLARHFSRITAEWEMKMEYVVQPDGTFRFDRADAIAAFARQRGL
                     SLFGHTLVWYANEPEGFAMLDETRQSFEGALQNYIAAMVNRYRGLAAGWDVVNEPVMV
                     NGEGLRESLWTRKLGQAGHFRVAFEAARAADPAAVLFVNDYHLESRPAKLRTYMQLIE
                     QLLKDGVPLGGIGCQTHCWSELPPGSIAATVRALAGFGLPIHLSEMDVSVVQGGPLAS
                     RADAERRQARLYAEAVEAFASLPESQQFAFTVWGLRDRDSWLRREDPSDTPLLFDDSG
                     RPKAAFAAVADGLRRRRTAG"
BASE COUNT          262 a          629 c          640 g          265 t
ORIGIN      
        1 gcgattccgc cggtgtgcag atcggcacgg tgatggaccc cgatccgacc atgcacgcca
       61 acgggcgggt gaccatcaag accagcaccg gcttcatcac ggtgccgcgc ggcagcctga
      121 ccctcgaggg tggggtggcc gtcagccgtc agaccgaggc cgaccttcgg gcttcgggcg
      181 cgaccaagcc gcagtaggcg agcgggcgtc ccggccgcgt cgatttcgcg ccgccgggac
      241 gcctcgtcgg cattgccccg ggggccgcgc gccgatagac atgcggccgt gagggcggcg
      301 cccctgccgc cggggaaaac ttgcgcatgg ctccggccag accaaatcga cgacagggcg
      361 ccggcgggac ctttctgtgg gacctgctga ccttcgaccg gctgatgacc ggcccggtga
      421 tccacctgat ctactgggcg ggtctgggcg tgatcatcat tggcttcttc ggggccatcg
      481 gcgccgcggt cggcgtggcg atgcaggaca ccaacatcat cggccggctg ctggcgctgc
      541 cggtggtcat cgccggcctg ctggtcggct tcgccctggg cctgctgtgg cggtcgttct
      601 gcgagttcta cgtggcgatc ttccgcatcg ccgacgacct gcgcgccctg cgccaggcca
      661 gcgacgccga ccatgtgctg aagaagccga tcagcccgcc gtccgtcgac gtctgagccc
      721 gtcggcgacc gcggcgaagg ccgccttcgg ccgccccgaa tcatcgaaca gcagcggcgt
      781 gtcgctggga tcctcgcgcc tgagccagct gtcgcggtcg cgaaggcccc agaccgtgaa
      841 ggcgaactgc tgggactcgg gcaggctggc gaacgcctcg accgcctcgg cgtagagccg
      901 cgcctgacgc cgttcggcgt ccgcccgcga cgccagcggg ccgccctgga ccaccgacac
      961 gtccatctcc gacaggtgaa tcggcaggcc gaagccggcc agggccctga ccgtcgcggc
     1021 gatcgatccg ggcggcagct cggaccagca gtgggtctgg cagccgattc cgccgagcgg
     1081 cacgccgtcc ttcagcagct gttcgatgag ctgcatatag gtccgaagct tcgctggccg
     1141 gctttcgagg tgatagtcgt tgacgaacag gaccgcggcg ggatcggcgg cccgggcggc
     1201 ctcgaacgcc acccggaaat ggccggcctg gcccagcttg cgggtccaca ggctctcgcg
     1261 aaggccctcg ccgttgacca tcaccggctc gttgaccacg tcccagccgg cggccaggcc
     1321 gcgatagcgg ttgaccatgg cggcgatgta gttctgcagg gcgccctcga agctctggcg
     1381 cgtctcgtcc agcatcgcga agccttccgg ctcgttggcg taccagacca gcgtatggcc
     1441 gaagaggctc aggccgcgct gccgtgcgaa ggcggcgatg gcgtcggccc ggtcgaagcg
     1501 gaacgtgccg tccggctgga cgacgtattc catcttcatc tcccactcgg ccgtgatccg
     1561 ggagaagtgc cgggcgagca ggtcggcgta ggcgggctcc tcgagcgagg ccgccctgac
     1621 ggcggttccg agcgggaacg gcgccaggga cttcaacggc gtgtcgacga ccgtctgggc
     1681 cgccgccggc tcccgcccgc aggcggccag cgcaagcccg gatgcgagta cgaggcggcg
     1741 ggaaggcggg gacagctcaa gctgccccaa gggacagctt gagctgtccc cgtctc
//
LOCUS       DAORNX010000553         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_47620_length_1793_cov_5.818182, whole genome shotgun sequence.
ACCESSION   DAORNX010000553 DAORNX010000000
VERSION     DAORNX010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47620_length_1793_cov_5.818182"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..539)
                     /locus_tag="PLO65_16565"
     CDS             complement(99..539)
                     /locus_tag="PLO65_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRD29912.1"
                     /translation="MTTRTAVLALFAGLALAASRPSTAATPAGSPTGVWLTPEDRGQV
                     QLFDCGDRLCGRIVTSDQLRANPDLKDANNRNPALRDRPLKALTLIRDFSGGPRVWSG
                     GSIYRPQDGGTYKGTLEMIDANTLKMTGCVVAPLCKTQVWKRVR"
     gene            complement(565..1278)
                     /locus_tag="PLO65_16570"
     CDS             complement(565..1278)
                     /locus_tag="PLO65_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD29913.1"
                     /translation="MILVRSLTFTLAYYLYSTVVALLMIPLFLAPRAWVTRVLTWYGA
                     GTTLLLRAICGIRVEVRGREHLPRGAALIAGKHQCMFDIFGSFAFLPDACFVAKKELM
                     VIPFLGWYATKGGMIVVDREGHSTALRKLVADARDRFKDARQLVIFAEGTRKAPGAPP
                     DYKPGIAALYRDLEMPVIPLALNTGVHWPAHGIMRYPGTIVFEFLPPIPAGLKRGEFM
                     RELETRIETASNALVAEGL"
     gene            complement(1275..>1793)
                     /locus_tag="PLO65_16575"
     CDS             complement(1275..>1793)
                     /locus_tag="PLO65_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRD29914.1"
                     /translation="TAGRLEEAILALEAAVRLLERGKGRRLLISGVNKEASRADILTV
                     SRAAKPIYDCCVDLGFTAADTLGNAREIAEWADGKGYDSLIVVTSDYHMPRAMLLVRA
                     AMPGATLIAYPVVTPPVDVRHWWRRSGDARRLAIEYCKYLAILARESVRGLGAGSDDT
                     PPAKAAAKGTKP"
BASE COUNT          248 a          611 c          663 g          271 t
ORIGIN      
        1 cgcggccgac gatctctatt cccgccggct gtcggtgcgg cgggcggtgg cgcggcccgg
       61 cgggcgggcg ccgcccggtc cgtcccgact gacggccgtt accgaacccg cttccagacc
      121 tgcgtcttgc acaggggtgc gaccacgcag ccggtcatct tcagggtgtt ggcgtcgatc
      181 atctcgagcg tgcccttgta ggtgccgccg tcctgcggcc ggtagatcga gccgccgctc
      241 cacacccgcg ggccgccgga aaagtcgcgg atcagcgtca gggccttgag cggccggtcg
      301 cgcagggcgg ggttgcggtt gttggcgtcc ttcaggtccg gattggcgcg cagctggtcg
      361 gaggtgacga tccgcccgca cagccggtcg ccgcagtcga acagctgcac ctggccgcgg
      421 tcctcgggcg tcagccagac gccggtcggc gatccggcgg gcgtggcggc ggtcgaggga
      481 cgggacgcgg ccagcgcgag gccggcgaac agggcgagga cggcggttcg ggtcgtcacg
      541 atcgggtctc cttgggcgtg gcggctagag gccttcggcc accagggcgt tgctggccgt
      601 ctcgatgcgg gtttcgagct cgcgcatgaa ctcgccgcgt ttgaggcccg ccgggatcgg
      661 cggcaggaac tcgaacacga tggttcccgg gtaccgcatg atcccgtgcg ccggccagtg
      721 gacgccggtg ttcagggcca gcgggatcac cggcatctcc aggtcacgat agagggcggc
      781 gatgcccggc ttgtagtcgg gcggggcgcc gggcgccttg cgggtgccct cggcgaagat
      841 caccagctgc cgggcgtcct tgaagcggtc gcgcgcgtcg gcgaccagct tgcgcagggc
      901 ggtggaatgg ccctcgcggt cgacgacgat catgcctccc ttggtggcgt accagccgag
      961 aaacgggatc accatcagct ccttcttggc cacgaaacag gcgtcgggca ggaaggcgaa
     1021 gctgccgaag atgtcgaaca tgcactggtg cttgccggcg atcagggccg cgcccctcgg
     1081 caggtgctcg cggccgcgca cctcgacccg gatgccgcag atcgcccgca gcagcagggt
     1141 cgtgccggcg ccgtaccagg tcagcacccg cgtcacccag gcccgggggg ccaggaacag
     1201 cgggatcatc agcagggcga cgacggtcga atagaggtag taggcgaggg tgaaggtcag
     1261 cgaccggacc aggatcaagg tttcgttccc ttcgcggccg ccttggccgg cggcgtgtca
     1321 tcggatccgg cgcccaggcc gcgcacgctc tcgcgggcca ggatcgccag gtatttgcag
     1381 tattcgatcg ccagtcggcg cgcatccccg ctgcggcgcc accagtggcg cacgtcgacc
     1441 ggcggcgtga ccaccggata ggcgatcagc gtcgcccccg gcatcgccgc ccggaccagc
     1501 agcatggccc gcggcatgtg gtagtcggag gtcaccacga tcaggctgtc atagcccttg
     1561 ccgtcggccc attctgcgat ctcccgcgcg ttgcccaggg tgtcggcggc ggtgaagccc
     1621 aggtcgacgc agcagtcgta gatcggcttg gcggcgcggc tgaccgtcag gatgtcggcc
     1681 cggctggcct ccttgttgac cccggagatc agcagccgcc ggcccttgcc gcgctcgagc
     1741 aggcgcaccg ccgcctcgag cgccaggatc gcttcctcca gtcggcccgc ggt
//
LOCUS       DAORNX010000554         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_47797_length_1787_cov_3.669746, whole genome shotgun sequence.
ACCESSION   DAORNX010000554 DAORNX010000000
VERSION     DAORNX010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47797_length_1787_cov_3.669746"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLO65_16580"
     CDS             <1..449
                     /locus_tag="PLO65_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29915.1"
                     /translation="LAATLWMASAAPVTATPAAAVAVSAPPQSVAGEPLFADIVKRAE
                     ALKTEVDGWKGKAGPLPGFDAFAARVAELSGLDMQAHLLLAKRGTDGDLKCILRGISQ
                     DLPVRLKEVETAADQRSRDMALKEMFWLLRDNVEVITTPPTATSGT"
     gene            complement(528..>1787)
                     /locus_tag="PLO65_16585"
     CDS             complement(528..>1787)
                     /locus_tag="PLO65_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD29916.1"
                     /translation="GPDGGARPSLLLALLFAFLGGVILNLMPCVFPILSMKATALAGH
                     AHEAKAARHQGLAYLAGVLATFLALAGTLIALKAAGAAVGWGFQLQSPVAVAVLAVLM
                     LLVALNLSGVFEIGTSLQGVGGRLADNSRGLLGSFLTGVLAVVVAAPCTAPFMGPAMG
                     FALTQSPLVSLAVFAFLGLGLAAPFVALSFAPALLRLMPRPGPWMETLRNVLAFPMYG
                     TAAWLTWVLAQQTGSLGLGMGLAAAVMAAFAAWLFGRSQRREKAPLQRGLSILALLAA
                     AGLIYGLAIAPRATVALTEAAPQTRADLPSEPWSPERVTALRAEGRPVFVNFTAAWCV
                     TCQVNDAAAISRPAVAEAFKTAGAVYLVADWTNRDATIAAALAEHGRAGVPLYLVYGA
                     DGAPPTVLPQLLTEGMVVQAIAKGAKR"
BASE COUNT          293 a          664 c          629 g          201 t
ORIGIN      
        1 cccttgccgc cactctctgg atggcctccg ccgcgccggt gacggcgacc ccggccgccg
       61 ccgtggcggt cagcgccccg ccccagtcgg tggccggcga gccgctgttc gccgacatcg
      121 tcaaacgggc cgaggccctc aagaccgagg tcgacggctg gaagggcaag gcgggcccgc
      181 tgcccggctt cgatgccttc gccgcgcggg tcgccgagct gtctggcctc gacatgcagg
      241 cccacctgct gctggccaaa cgcggcaccg acggcgacct gaagtgcatc ctgcgtggca
      301 tctcgcagga cctgccggtg cggctcaagg aagtcgaaac cgccgccgac cagcgcagcc
      361 gcgacatggc cctgaaggag atgttctggc tgctgcgcga caacgtcgag gtcatcacca
      421 ccccgccgac ggccaccagc gggacatagc gcgccacccc ttcccgtcat cgtcggaaca
      481 agtccgacga tgacgaaagc cgaagggtcg ggcgggcgtt cccgccccta acgcttcgcc
      541 cctttggcga tggcctggac gaccatcccc tcggtcagca gttgcggcag caccgtcggc
      601 ggcgcgccgt ccgcgccata gaccagatac agcggcaccc cggcgcggcc gtgctcggcc
      661 agcgccgcgg cgatggtcgc gtcccggttg gtccagtcgg ccaccagata gaccgcgccg
      721 gcggtcttga aggcctcggc cacggccggt ctggaaatgg ccgccgcgtc attgacctgg
      781 caggtgacgc accaggcggc ggtgaagttg acgaacaccg gtcggccctc ggcgcgcagg
      841 gccgtgaccc gctcgggcga ccagggctcc gatggcaggt cggccctggt ctgcggggcc
      901 gcctcggtca gggcgacggt tgcgcgcggg gcgatggcca ggccgtagat caggcccgcc
      961 gcggcgagca gggcgaggat cgacaggccg cgctgcagcg gggccttctc gcgtcgctgg
     1021 gaccgcccga acagccaggc ggcgaacgcc gccatgaccg ccgcggcgag acccatcccg
     1081 aggcccagcg atccggtctg ctgcgccagc acccaggtca gccaggccgc cgtgccgtac
     1141 atcgggaagg ccaggacatt gcgcagcgtc tccatccacg gaccgggacg gggcatcagc
     1201 cgcaacaggg ccggcgcgaa cgacagggcg acgaacggcg ccgcgagccc aaggccgagg
     1261 aaggcgaaca ccgccagcga caccagcggg ctctgggtca gggcgaagcc catcgccggc
     1321 cccatgaagg gcgcggtgca gggggccgcc accacgaccg ccagcacgcc ggtcaggaag
     1381 ctgcccagca ggccgcggct gttgtcggcc agtcgtccgc cgacaccctg caatgacgtg
     1441 ccgatctcga acacccctga cagattcagg gccaccagca gcatcagcac cgccagcacg
     1501 gccaccgcca cgggcgactg cagctggaag ccccagccga cggcggcgcc ggcggccttg
     1561 agcgcgatca gggtcccggc cagggccagg aaggtcgcca gcacgcccgc gagataggcc
     1621 aggccctggt gccgcgccgc cttggcctcg tgggcgtgcc cggccaacgc ggtcgccttc
     1681 atcgacagga tcgggaacac gcagggcatc aggttgagga tcactccgcc gagaaaggcg
     1741 aacagcaacg ccaggagcag actcgggcgg gcgccgccgt ccggtcc
//
LOCUS       DAORNX010000555         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_48255_length_1772_cov_4.232382, whole genome shotgun sequence.
ACCESSION   DAORNX010000555 DAORNX010000000
VERSION     DAORNX010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48255_length_1772_cov_4.232382"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..629
                     /locus_tag="PLO65_16590"
     CDS             126..629
                     /locus_tag="PLO65_16590"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRD29917.1"
                     /translation="MRDAAGDPAAARLFNNAAQAWNHGLFWDAMSPDGGAPGNDLAAA
                     IATAFGDRAGLKTAFVKAGAEHFGSGWVWLLAEGDRLRVLATHDAANPLTLDGAVPLT
                     VCDLWEHAYYLDHQNDRKGFLEAWFDGLLNWRLADSQFAAARGRGQAWRYPEPVEAGG
                     TARHAMR"
     gene            680..850
                     /locus_tag="PLO65_16595"
     CDS             680..850
                     /locus_tag="PLO65_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRD29918.1"
                     /translation="MLRWALIFLVVALIAAFLGFGGLAGTAVDIAKILFFIFLVLFLV
                     SAVMHLVRGRAP"
     gene            complement(916..1707)
                     /locus_tag="PLO65_16600"
     CDS             complement(916..1707)
                     /locus_tag="PLO65_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD29919.1"
                     /translation="MTVRLAHLSDIHFGGENAAAVGGAAAWIAEHRPDLAVITGDVTR
                     QGDEREFAAARAWINSLAAEALVLPGNHDTPYFSLPKRLFAPWRRYERHFGPLERTGL
                     HRRGLAIASINTARGAQLRANWSKGAISARQVDHAIDALAAAEPGALKVVACHHPLVE
                     MIGGPMSGLVHGGHDAALAFSAARVDLVLTGHVHAPFALPYPGGDGRTWAVGASTLSI
                     RERGAPIGFNCIEVEAACVRVTALAWRGSHFEPWRTWTIDRRPTG"
BASE COUNT          250 a          628 c          637 g          257 t
ORIGIN      
        1 ctacttcaac ggcgagaccg tccggctcga cggcgcgatc cgcatgccgc cgcgttagat
       61 ccggacaacc ccggggaggg ctcgtcgagg cccgcggcgg ccatccggac agccttgaat
      121 cggtggtgcg cgacgccgcc ggcgacccgg ccgccgccag gctgttcaac aacgccgccc
      181 aggcctggaa ccacggtctg ttctgggacg ccatgagccc cgacggcggc gcgccgggga
      241 acgacctggc cgcggccatc gccaccgcgt tcggcgaccg ggccgggctg aagaccgcct
      301 tcgtcaaggc cggcgccgag catttcgggt cgggctgggt ctggctgctg gccgagggcg
      361 accggctgcg ggtcctcgcc acccacgacg ccgccaaccc cctgacgctg gacggcgccg
      421 tgccgctgac cgtctgcgac ctgtgggagc acgcctacta tctcgaccac cagaacgacc
      481 ggaaggggtt cctcgaggcc tggttcgacg gtctgctcaa ctggcggctg gccgacagcc
      541 agttcgcggc ggcccgcggg cgcggccagg cctggcgata tccggagcct gtagaggcag
      601 gtggaacggc gcgccacgcg atgcgttgag agaacacggg ctgtccaata ccgcctgatc
      661 gtgaatggag agacgcagca tgctcagatg ggcgctcatt tttctggtcg tggcgctgat
      721 cgccgcgttc cttggctttg gcggacttgc cggcacggcc gtcgatatcg ccaagatcct
      781 gttcttcatc ttcctggtgc tgttcctggt ttcggccgtg atgcacctgg tccggggccg
      841 ggcgccctga cgacggcgct ggccggacga tgacgacggc ggcggggcga tcccgccgcc
      901 gttttcattt gcggttcagc ccgtcggccg ccggtcaatc gtccaggtcc gccagggttc
      961 gaagtgcgag ccgcgccagg ccagggcggt gacgcgcacg caggccgcct cgacctcgat
     1021 gcagttgaag ccgatcgggg cgccacgctc gcggatcgac agggtgctgg ccccgaccgc
     1081 ccaggtgcgg ccgtcgccgc ccggataggg cagggcgaac ggcgcgtgca catggcccgt
     1141 cagcaccagg tcgacccgcg ccgcggaaaa ggccagggcc gcgtcgtggc cgccatggac
     1201 caggccgctc atcggcccgc cgatcatctc caccagcgga tggtggcagg cgacgacttt
     1261 cagcgccccg ggctccgccg ccgccagggc gtcgatcgcg tgatccacct gccgcgccga
     1321 gatcgcgccc ttcgaccagt tggcgcgcag ctgtgcgccg cgggcggtgt tgatcgaggc
     1381 gatggccagg ccgcgacgat gaaggcccgt ccgttccagc gggccgaaat gccgctcgta
     1441 ccgccgccat ggcgcgaaca gccgcttggg caggctgaag tagggcgtgt cgtggttgcc
     1501 cggcaggacc agcgcctccg ccgcgaggct gttgatccag gcacgggcgg cggcgaactc
     1561 gcgctcgtcg ccctggcggg tgacgtcgcc ggtgatcacc gccaggtcgg gccggtgctc
     1621 ggcgatccag gccgccgcgc cgccgaccgc cgccgcgttc tcgccgccga agtgaatatc
     1681 cgacaggtgg gccaggcgga cagtcacgcg tcgacctccg catcgcgcgg cgtcagggcg
     1741 cggaaggcct tcggccgaaa ccggaaattg ac
//
LOCUS       DAORNX010000556         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_48531_length_1764_cov_9.583967, whole genome shotgun sequence.
ACCESSION   DAORNX010000556 DAORNX010000000
VERSION     DAORNX010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48531_length_1764_cov_9.583967"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1209)
                     /locus_tag="PLO65_16605"
     CDS             complement(205..1209)
                     /locus_tag="PLO65_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRD29920.1"
                     /translation="MKVAAVKAPGGLQNIVIEERDRPAPGPGEILVRVRASSLNFHDF
                     AVVAGMIRVPDGRIPMSDGAGEVVEVGPGTRLFKVGDQVLSTFFPNWPAGGPSLPRLL
                     GVPGDSAEGFAAEYVAMPETAFTRIPHGWTPAQAATLPCAALTAWRALMVEARIKPGD
                     IVLTQGTGGVSIFALQLAKAAGATVISTSSSPEKLARLKALGADHLINYKEQTDWGAA
                     AMALTGGRGVDAVVEIGGPGTMGQSIQACRIGGHISLIGVLTGVSGEIPTALAMSKNV
                     CIQGITVGSRQDQEDMIEAIEASGLAPVIDSTFPLDQIAAAFAHQMSQTHFGKICLEF
                     "
     gene            complement(1255..>1764)
                     /locus_tag="PLO65_16610"
     CDS             complement(1255..>1764)
                     /locus_tag="PLO65_16610"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD29921.1"
                     /translation="RGYIYLPLLNYTEFNFDHIHAMMQDPNTVLSLSDGGAHCGVICD
                     ASFPTYMLTHWVRDRKRGPRLPLEKVVSMQTRDTARLYGLNDRGVLAVGMKADLNVID
                     FDRLAIRAPEMVFDLPADGRRMIQRADGYVATIVSGVVTWENGAATGAMPGKLIRGGR
                     QSAVALAAE"
BASE COUNT          269 a          587 c          641 g          267 t
ORIGIN      
        1 gaggactcgc tccgtggccg gggcgggcgt cggaggcgcg gccggcggct gcgaggtgct
       61 ggcgcaggcc gacaggacga atgcggccaa gagccccgcg atgcgcttca tgagtccccc
      121 caggacatct ttcccaccca gatagggcgg acgcgccggg cggccagccg cccgtgggcg
      181 tcgccgtccg gcgtcggctc gcgatcagaa ctcgaggcag atcttgccga agtgtgtctg
      241 cgacatctga tgggcgaagg ccgccgcaat ctggtcgagc gggaaggtcg agtcgatcac
      301 cggggccagg ccactggcct cgatagcctc gatcatgtct tcctggtcct ggcgcgagcc
      361 cacggtgatg ccctggatgc agacgttctt ggacatcgcc agcgcggtcg ggatctcgcc
      421 cgacacgccg gtcagaacgc cgatcaggga gatgtggccg ccgatgcggc aggcctggat
      481 cgactggccc atggtgcccg gcccgccgat ctcgaccacc gcgtcgactc cgcgcccgcc
      541 ggtcagggcc atggcggccg cgccccagtc ggtctgttcc ttgtagttga tcagatggtc
      601 agccccgagc gccttcagcc gggcaagctt ttccggcgag gacgaggtcg agatcaccgt
      661 cgcgccggcc gccttggcca gttgcagcgc gaagatcgac accccgccgg tgccctgggt
      721 caggacaata tcgccgggct tgatgcgcgc ctcgaccatc agggcccgcc aggcggtcag
      781 ggccgcgcag ggcagggtgg cggcctgggc gggggtccag ccgtgaggga ttcgggtgaa
      841 ggccgtctcg ggcatggcca catactcggc cgcgaaaccc tcggcgctgt cgccgggcac
      901 gccgagcagg cgcggcaggc tcgggccgcc ggccggccag ttggggaaga aggtcgacag
      961 cacctggtcg ccgaccttga acagccgggt tcccggcccg acctcaacca cctcgccggc
     1021 gccgtcggac atcggaatac ggccgtccgg cacccggatc atgcccgcga cgacggcgaa
     1081 atcatggaag ttcagggagc tggcgcgaac ccgcacgagg atctcgccgg ggcccggcgc
     1141 cggccggtca cgctcctcga tgacgatgtt ctgtagcccg cccggggctt tgacggcggc
     1201 gactttcatg ggtgggtctc ggtttggact ggaggtggag gggaacagac ggagtcactc
     1261 ggcggccagg gccacggcgg actggcggcc gccgcggatc agcttgccgg gcatggcgcc
     1321 ggtggccgcg ccgttttccc aggtcaccac gccgctgacg atggtcgcca catagccgtc
     1381 cgcgcgctgg atcatgcgac ggccatcggc gggcaggtcg aagaccatct cgggcgcccg
     1441 gatggccagc cggtcgaagt cgatgacatt gaggtcggcc ttcatgccga cggccagcac
     1501 gccccggtcg ttcaggccat agagacgggc ggtgtcgcgc gtctgcatcg acaccacctt
     1561 ctccagcggc agcctcgggc cgcgtttgcg atcgcgcacc cagtgggtca gcatgtaggt
     1621 cgggaagctg gcgtcgcaga tcacgccgca atgggcgccg ccgtcggaca ggctgagcac
     1681 cgtgttcgga tcctgcatca tcgcgtggat gtggtcgaag ttgaactcgg tgtagttcag
     1741 cagcggcagg tagatgtagc cgcg
//
LOCUS       DAORNX010000557         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_48587_length_1762_cov_6.418278, whole genome shotgun sequence.
ACCESSION   DAORNX010000557 DAORNX010000000
VERSION     DAORNX010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48587_length_1762_cov_6.418278"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLO65_16615"
     CDS             complement(<1..377)
                     /locus_tag="PLO65_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HRD29922.1"
                     /translation="MIPWEHLATAVVPGDGGELRLMRRGTEYSIMAGSIELMNSRLSG
                     SEEALATLAHERLAGVAAPRVLIGGLGMGFTLRAALGVFGPGARIVVAELVPAVIAWA
                     RGPLAGLYGDSLAAGWADWSWDTT"
     gene            complement(382..1179)
                     /locus_tag="PLO65_16620"
     CDS             complement(382..1179)
                     /locus_tag="PLO65_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRD29923.1"
                     /translation="MALGRALAAIAALALLCFGTLAQAAPAMPALNGRRVADEAGILS
                     PSSEAALTRKLADLETATGREFAIATLPSLQGYSIEDFGYRLGRTWGVGSGQRDDGLL
                     LIVAPKERKVRIEVGYGLEGVMTDALAGLIIRVTILPKFRDGDLEGGIVAGADAIINQ
                     LKLPDEDATAIARKAHREADNDDWWVRPLLKFAIVMFATVIIPLFWSIFSGLFQAPWD
                     RGPREIRRRSRGLVDWSAGSYGGGDTSGGGYFGGGGSFGGGGASGSW"
     gene            complement(1184..>1762)
                     /locus_tag="PLO65_16625"
     CDS             complement(1184..>1762)
                     /locus_tag="PLO65_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRD29924.1"
                     /translation="FREGALLDGSSAGALLLGFQPQTLTALFGGWTVGHASASDATIL
                     AALTTYVALQAAVFVLAALIVAVPPVRRALTPASLKAERVHRAAMEQFLSHGLHATRD
                     RTGVLLFASLAERRAEVVADEGIYKVTDPAVWDEVVALLIGGLKKRKAADGFVAAVEL
                     SGRILAAHVPPGADNPNELPDRLVELPGPGKN"
BASE COUNT          302 a          637 c          580 g          243 t
ORIGIN      
        1 gtggtgtccc acgaccagtc cgcccagccg gcggccaggc tgtcgccgta gaggccggcc
       61 agcggccctc tggcccaggc gatcaccgcc gggaccagct cggccacgac gatcctcgcc
      121 cccggcccga agacgccgag cgccgcgcgc agggtgaaac ccatgcccag cccgccgatc
      181 agcacgcgcg gcgcggcgac gccggccagt cgctcatggg ccagcgtggc cagcgcctct
      241 tcagagccgc tcagccggct gttcatcagc tcgatcgacc cggccatgat cgaatattcg
      301 gttccgcgcc gcatcagacg cagctcgccg ccgtcgccgg gcacgacagc cgtggccaga
      361 tgttcccagg ggatcaagag ctcaccaact ccctgaagcg ccgccgccgc caaaggaccc
      421 gccgccgccg aagtagccgc caccggatgt atcaccaccg ccgtagctcc cggcgctcca
      481 gtccaccagc cctcgcgaac gtcgccgaat ctcccggggg ccacggtccc agggggcttg
      541 gaacaggccg gagaagattg accagaaaag cgggatgatc accgtggcga acattacgat
      601 ggcgaacttg agcagcggtc gaacccacca gtcgtcattg tcggcctctc gatgggcctt
      661 gcgtgcaatg gccgtcgcat cttcatcggg gagcttgagc tgattgatga tggcgtcggc
      721 gcccgcgaca atcccgcctt caaggtcacc gtcgcggaac ttcggcagaa tggtcacccg
      781 aatgatgagc ccggccagag cgtcggtcat cacaccctcc aggccgtagc ccacctcgat
      841 gcgaaccttt cgttccttcg gagcgacaat cagcaggagg ccatcgtcgc gttgccccga
      901 tccgacaccc cacgtacggc ccaggcgata gccaaagtcc tcgatggaat agccctgcag
      961 ggaaggcagc gtggcgatgg cgaattcccg ccctgttgcg gtttcaagat ccgccagctt
     1021 ccgggtcaga gcagcttccg aagacggtga gagaatcccg gcctcatcgg ccactcgcct
     1081 gccgttgagc gccggcatcg cgggggctgc ctgagcaagc gttccgaagc atagaagcgc
     1141 cagcgcggct atcgccgcca gtgcccggcc gagggccacg acgctagttc ttgcccggac
     1201 ccggcagctc caccagccgg tcgggcagct cgttgggatt gtcggcgccc ggcggcacat
     1261 gcgccgcgag gatgcggcca gacagctcca cggcggcgac gaagccgtcg gccgccttgc
     1321 gcttcttgag gccgccgatc agcagggcga ccacctcgtc ccacaccgcc gggtcggtga
     1381 ccttgtagat gccctcgtcg gccaccacct ccgcccgccg ctcggccagc gaggcgaaca
     1441 gcagcacgcc ggtgcggtcg cgggtggcgt gcaggccgtg gctgagaaac tgctccatgg
     1501 ccgcgcggtg cacgcgctcg gccttcaggc tggccggggt cagggcccgg cgcacgggcg
     1561 gtacggccac gatcagggcg gcgaggacga acaccgccgc ctgcagggcg acataggtgg
     1621 tcagggccgc caggatggtg gcgtcgctgg ccgaggcgtg gccgacggtc cagccgccga
     1681 acagggcggt cagcgtctgg ggctggaaac ccagcaacag cgccccggcg ctggaaccgt
     1741 ccaacagtgc gccctctctg aa
//
LOCUS       DAORNX010000558         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_48778_length_1756_cov_4.645503, whole genome shotgun sequence.
ACCESSION   DAORNX010000558 DAORNX010000000
VERSION     DAORNX010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48778_length_1756_cov_4.645503"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLO65_16630"
     CDS             <1..1475
                     /locus_tag="PLO65_16630"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRD29925.1"
                     /translation="FVQGTLADVLSHYGYVDLELRPSPYVVSQMNGAYYWLPDFIAGQ
                     HPLDGPADVAAWHERLAGLAGVLDSETGRIGDDAARGIVPPRFVIDRTLAQIAALRDG
                     SPGASPLIAPAAARAAQNGLGDILPSAELTFREVIAPALSRQIDALDALRSRAVDTAG
                     VWRLPDGEAYYAAALRANTTVDDSPARLHEAGLAQVEDLLARIDQSLAAQGLTQGSVG
                     ARIRAMNSDVRFLATDDDAGRDRLLATARQALERVSARLPSAFLTTGVEPVIVERVPQ
                     TIEQGSPGAFYQEGAPGEPGVFRLNLSSVAEHPLWRLPTLAHHEGVPGHHFQAGVLRQ
                     AGALPLFRRMAVFSAYSEGWALYAQQVADELEVFEDDPFARIGYLQSQLFRAARIVVD
                     TGLHFRRWERAQAVAWMVDRIGEPAESAGREIDRYCVYPGQACSFKVGANAIVAAREA
                     ARTRLGPAFDIRRFHERVLTSGPVPMAVLASMTAQWDGAA"
BASE COUNT          238 a          626 c          636 g          256 t
ORIGIN      
        1 ggttcgtcca gggcacgctc gcggatgtgc tgagccacta cggctacgtc gatctcgaac
       61 tgcggcccag cccctatgtc gtcagccaga tgaacggcgc ctactactgg ctgccggact
      121 tcatcgccgg ccagcacccg ctggacgggc cggccgatgt ggcggcctgg catgagcgcc
      181 tggcgggcct ggccggggtc ctggattccg aaacaggccg gatcggggat gacgccgcac
      241 gcgggattgt cccgccccgc tttgtcatcg accggaccct ggcgcagatc gccgccctgc
      301 gggacgggtc gccgggcgcc tctccgctga tcgcgccggc ggcggcccgc gccgcgcaaa
      361 acggcctggg cgatatcctg ccttcggcgg aattgacgtt cagggaggtc atcgcaccgg
      421 ccctgtcgcg ccagatcgac gcgctggacg ccctgaggtc cagggcggtg gacacggccg
      481 gcgtctggcg gcttccggac ggcgaggcct actacgccgc ggccctgcgc gcgaacacca
      541 cggtcgacga ctctcccgcc cggttgcacg aggcgggact ggcgcaggtc gaggacctgc
      601 tggcccgcat cgaccagagc ctggccgccc agggcctgac gcagggatcg gtcggggcgc
      661 gcatccgggc gatgaacagc gacgtccgct tcctggcgac cgacgacgac gcaggccgcg
      721 accggctgct ggcgacggcg cggcaggcgc tggaacgggt gtccgccagg ctgccctcgg
      781 ccttcctgac gaccggggtc gagccggtga tcgtcgaacg cgtcccgcaa accatcgaac
      841 agggatcgcc cggcgccttc taccaggagg gggctcccgg cgagcccgga gttttcagac
      901 tgaacctctc gagcgtcgcc gagcatcccc tgtggcggct gcccacgctc gcgcaccatg
      961 aaggcgtacc cggccaccac ttccaggccg gcgtgctgcg ccaggcgggc gccctgccgc
     1021 tgtttcgccg gatggcggtg ttttccgcct acagcgaggg gtgggccctg tatgcgcagc
     1081 aggtggcgga tgagctggag gtgttcgagg acgacccgtt cgcccgcatc ggctacctgc
     1141 agtcgcaact gttccgcgcg gccaggatcg tcgtcgacac gggccttcac ttccggcgct
     1201 gggagcgggc ccaggcggtg gcgtggatgg tggacaggat cggcgaaccg gccgagtcgg
     1261 cgggccggga aatcgaccgc tactgcgtct atccgggcca ggcctgcagc ttcaaggtcg
     1321 gcgcgaacgc catcgtcgcg gcccgggagg ccgcgaggac gcggctgggc cccgcctttg
     1381 acattcgccg gttccatgag cgggtcctga cctcgggacc ggtgccgatg gcggtcctgg
     1441 cgtcgatgac ggcccaatgg gacggggccg catgacccct cctgcgccca gccgggacgg
     1501 gccgccgggc tgtcatcgcg atccgggcgc ccctggcgag accatggcgg gaggtcccgc
     1561 cagcccgggc atccgcgcct gaccggggtc cccgtttccg gccgcgaccg ggccgccgcc
     1621 ctcgacgggc catccatatc ggtcctgatg atcatcctgc ggttccggcc cgagatcggc
     1681 ggggcggaac aggcgctcga acggctggcg acggaacttg ccgatcgcca cgtccggacc
     1741 acggtggtca cctttc
//
LOCUS       DAORNX010000559         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_48857_length_1754_cov_7.502649, whole genome shotgun sequence.
ACCESSION   DAORNX010000559 DAORNX010000000
VERSION     DAORNX010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48857_length_1754_cov_7.502649"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLO65_16635"
     CDS             <1..759
                     /locus_tag="PLO65_16635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD29926.1"
                     /translation="LKDRIIVITGAASGIGRAMAHRFAAEGPKLIVCADRDEAGARAT
                     AEAVGGLAFRVDVSRETDIQGLIATVETDHGPIDLFCSNAGIGVAGGAEASNDDWQRI
                     WDINVMAHVWAARHLVPLMAARGGGYLLNTASAAGLLTQIGSAPYAVTKHAAVGLAEW
                     LAITHGDQGIKVSVLCPQAVRTAMTAGNPDGVASVDGMMEPETLAEMVVRSIAAEEFL
                     ILPHPQVLDYMRNKTADYGRWIGGMRKLNRRYDG"
     gene            848..>1754
                     /locus_tag="PLO65_16640"
     CDS             848..>1754
                     /locus_tag="PLO65_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRD29927.1"
                     /translation="MKGPAIFLAQFAGDAAPFNSLEAIARWAGDLGYKGVQIPTWDRR
                     LFDLKLAAESDAYCDEVKGVLAAAGLEVSELSTHTQGQMVAVHPAYDEMFDGAAPARV
                     RGNPAARQAWAVEQVLLGAKASRRMGLEAHVTFSGSLAWPYLYPYPQRPAGLIEEAFD
                     ELARRWRPILDAFDEVGVDLCYEIHPSEDLFDGTTVEMFWDRVDHHPRACLLYDPSHF
                     LLQQLDYLEYIDLYHERIRMFHVKDAEFRPTGRQGVYSGYAPWKERAGRFRSLGDGQI
                     DFKAIFSKFAQYGYRGWAVLEWECAL"
BASE COUNT          271 a          601 c          615 g          267 t
ORIGIN      
        1 ctcaaggacc gcatcatcgt catcaccggc gccgccagcg gcatcggccg ggccatggcg
       61 catcgcttcg ccgccgaggg tccgaagctg atcgtctgcg ccgaccgcga cgaggcgggc
      121 gccagggcca cggccgaggc cgtcggcggc ctggcgttcc gggtcgacgt cagccgcgag
      181 accgacatcc agggtctgat cgcgacggtc gagaccgacc acggcccgat cgacctgttc
      241 tgctccaacg ccgggatcgg agtcgcgggc ggggcggagg cctccaacga cgactggcag
      301 cggatctggg acatcaacgt catggcccat gtctgggccg cccggcatct ggtcccgctg
      361 atggcggccc gcggcggcgg ctacctgctc aacaccgcct cggccgccgg cctgctgacc
      421 cagatcggct cggcgcccta cgctgtgacc aagcacgcgg ccgtgggcct cgccgaatgg
      481 ctggccatca cgcacggcga ccagggcatc aaggtctccg tgctctgtcc ccaggcggtg
      541 cgcacggcga tgaccgccgg caatcccgac ggcgtggcca gcgtcgacgg catgatggag
      601 ccggagactc tcgccgagat ggtcgtccgc tcgatcgcgg cggaggagtt cctgatcctg
      661 ccgcacccgc aggtgctgga ttacatgcgc aacaagaccg ccgactacgg ccgctggatc
      721 ggcggcatgc gcaagctcaa ccggcgctac gacggttagc ccgctcgcca atggtctggc
      781 ccatgctagc cttcgcggcg acaaggaagg ggccgcccga tgcaggcggc ccatcgaggg
      841 aggacgcatg aaggggccgg ctattttcct ggcgcaattc gcgggtgacg cggcgccgtt
      901 caacagcctc gaggccatcg cgcgctgggc cggcgacctt ggctacaagg gcgtgcagat
      961 tcccacctgg gaccgacggc tgttcgacct gaagctggcc gccgagagcg acgcctactg
     1021 tgacgaggtg aagggcgttt tggccgcggc cgggctggag gtctccgagc tgtcgaccca
     1081 cacccagggc cagatggtcg cggtccatcc ggcctacgac gagatgttcg acggcgcggc
     1141 gccggcccgg gtgcgcggca atccggcggc gcgacaggcc tgggcggtcg agcaggtgct
     1201 gctgggcgcg aaggcgtcgc ggcggatggg gctggaggcc catgtcacct tctcgggctc
     1261 gctcgcctgg ccctacctct atccctatcc gcagcggccc gccgggctga tcgaggaagc
     1321 cttcgacgag ctggcccggc gctggcggcc gatcctcgac gccttcgacg aggtcggcgt
     1381 cgatctctgc tacgagattc atccgagcga ggacctgttc gacggcacga cggtggagat
     1441 gttctgggac cgcgtggatc accatccccg cgcctgcctg ctctacgatc cgagccactt
     1501 cctgctgcag cagctggact atctcgaata catcgacctc taccacgagc ggatccggat
     1561 gtttcacgtc aaggacgccg agttccggcc gaccgggcgg cagggggtct acagcggcta
     1621 cgcgccgtgg aaggagcggg ccgggcggtt ccgctccctg ggcgacggcc agatcgactt
     1681 caaggccata ttctccaaat tcgcccagta cggctatcgc ggctgggcgg tgctggagtg
     1741 ggaatgcgcg ctga
//
LOCUS       DAORNX010000560         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49159_length_1745_cov_4.488166, whole genome shotgun sequence.
ACCESSION   DAORNX010000560 DAORNX010000000
VERSION     DAORNX010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49159_length_1745_cov_4.488166"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..409
                     /locus_tag="PLO65_16645"
                     /pseudo
     CDS             1..409
                     /locus_tag="PLO65_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            482..1111
                     /gene="tmk"
                     /locus_tag="PLO65_16650"
     CDS             482..1111
                     /gene="tmk"
                     /locus_tag="PLO65_16650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRD29928.1"
                     /translation="MSRGKFITFEGGEGTGKSTQSRILTARLLAEGRTAIRTREPGGS
                     DGAEALRSLLVEGRADRWSPLSETLMMNAARRDHLERTIVPALDAGDWVVCDRFADST
                     RAYQGAGGGVPAEFIATVERAVVGDNCPDLTLVFDLPPEIGLERAFGRDMFEVRFETK
                     DFAFHTRLREAYLGIARAEPERCVVIDASGTEDQVADLVWNTVMDRLGR"
     gene            1108..>1456
                     /locus_tag="PLO65_16655"
     CDS             1108..>1456
                     /locus_tag="PLO65_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRD29929.1"
                     /translation="MTDGPVHPRDAFALEGAEVQERAFLDALDRGRLHHAWLLTGPEG
                     VGKATFAFRAARRLLGARPDPANGLLGSLASDPVVRQVSARSHPDLMVLERLGEDGKL
                     RKVIPVDEARRLPE"
     assembly_gap    1457..1476
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1477..>1745
                     /locus_tag="PLO65_16660"
     CDS             <1477..>1745
                     /locus_tag="PLO65_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRD29930.1"
                     /translation="PYRAAIIDAADDLNVNSANAILKTLEEPPPRGVILLVSHAPGRL
                     LPTIRSRCRRLGFTAPGEALTTAFLTARSDLTPEQAARLARMAGGA"
BASE COUNT          242 a          643 c          602 g          238 t
ORIGIN      
        1 cccttcgccc tcgggaccgg ggcccacggc cgcgcccggc ggtcggaccc ccgcgaccat
       61 gcggccctac caggtgcgcg gcgtctggta cacgccgcgc tacgatccca actacgacga
      121 gaagggtctc gccagctggt acggcgccca gttccacaac cgcagcacgg ccaacggcga
      181 gccgttcgac atgacccgcc cgtcggccgc ccacaagacg ctgccattgc cctgcatcgt
      241 cgaggtcacc gacctggata ccggccgcaa gatccgcgtt cgggtcaacg accgggggcc
      301 gttcgtccag gggcggatca tcgatctgtc gcgcgccgcc gccgaacagc tgggcatcgc
      361 gggcaagggc gtcgcccggg tgcgggtgcg ctatctgggc gcggcgccgg ccctgcgata
      421 gtccacagtt ccgcgtccgg ccattctgga catcgacgcc gttccgtccg atggaggagc
      481 cgtgtcgcgc ggaaaattca tcaccttcga aggcggggag gggaccggga aatccaccca
      541 gtcccgcatc ctgacggccc ggctgctggc cgagggccgg acggcgattc gcacgcgcga
      601 accgggcgga tccgacgggg ccgaagcgct gcgcagcctg ctggtcgagg ggcgcgccga
      661 ccgctggtcg ccgctgtcgg aaaccctgat gatgaacgcc gcccggcgcg accatctcga
      721 acggaccatc gtccccgcgc tcgacgcggg cgactgggtg gtctgcgacc ggttcgccga
      781 ctcgacccgc gcctaccagg gagccggcgg cggcgtgccg gccgagttca tcgcaaccgt
      841 ggaacgggcg gtggtgggcg acaactgtcc cgacctgacc ctggtgttcg acctgccgcc
      901 tgagatcggc ctggagcgcg cgttcgggcg cgacatgttc gaggtgcgct tcgagaccaa
      961 ggacttcgcg tttcacacgc gcctgcgcga ggcctatctg ggcatcgccc gcgccgagcc
     1021 tgagcgctgc gtggtgatcg acgcctccgg aaccgaggac caggtcgccg atctggtctg
     1081 gaacacggtc atggaccggc tgggccgatg accgacgggc ccgtccatcc gcgcgacgcc
     1141 ttcgcgctcg agggggcaga ggtccaggag cgcgcgtttc tcgacgcgct cgaccggggc
     1201 cggctgcacc acgcctggct gctcaccggg ccggagggcg tcggcaaggc caccttcgcc
     1261 tttcgcgccg cccgcagact gctcggcgcc cgccccgatc cggccaacgg cctgctgggg
     1321 tcgctggcct cggacccggt ggtccgccag gtgtcggccc gctcccaccc ggacctgatg
     1381 gtcctcgagc ggctcggcga ggatggcaag ctgcgcaagg tcatcccggt ggacgaggcc
     1441 cgccggttgc cggaatnnnn nnnnnnnnnn nnnnnnccct accgggcggc gatcatcgac
     1501 gcggccgacg acctgaacgt caattccgcc aacgccattc tcaagaccct ggaggagccg
     1561 ccgccgcgcg gggtcatctt gctggtcagc cacgcgccgg gacggctgct gccgacgatc
     1621 cggtcccgtt gccgccggct gggtttcacc gcgcccggcg aggccctgac caccgccttc
     1681 ctgaccgccc gcagcgacct cacccccgag caggccgccc ggctggcgag gatggccggc
     1741 ggcgc
//
LOCUS       DAORNX010000561         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49231_length_1743_cov_7.998815, whole genome shotgun sequence.
ACCESSION   DAORNX010000561 DAORNX010000000
VERSION     DAORNX010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49231_length_1743_cov_7.998815"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLO65_16665"
     CDS             complement(<1..352)
                     /locus_tag="PLO65_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29931.1"
                     /translation="MRRLSLITALSLLLAGPSLAQEGDRPDSRQDRGETQVRPNLRDA
                     VTAPLDDLNLKHTAIPDVLKRAVAGPYDMDGLTRCEAIANEIGRLDEALGPDLDEPPP
                     PDTRTRGQKLKQAAG"
     gene            475..1515
                     /locus_tag="PLO65_16670"
     CDS             475..1515
                     /locus_tag="PLO65_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD29932.1"
                     /translation="MPGTTRRWVLARHVEGAPTPDDFRLEETPLGELADGKFLSRVIL
                     GSVDPGTRSRLSGGDSYAGAMKIGEPMDGFCVAQVIESRHPNFAVGDLIAAGGGWREH
                     FVSDGRGFIQKITDRRVPLGCWIGVLGVPGLTAWFGLNRVGQAKEGETLLVTSAAGPV
                     GATAGQIGKKLGLRVVGVAGGPRKCAWLQDEARFDAVIDYKAEPDLTAAIKAACPEGV
                     DVLFDNVGNEMVDRVLPLMKLRGRVVVSGQVADYNVPVEQRYGLKHTDVFITHRVRME
                     GLVVFDDIRQFAAAQSQMADWIADGSLKFAIEEFDGLERAAEAFCGLFRGENFGRRLV
                     RVSPDPSWEDPA"
     gene            1515..>1743
                     /locus_tag="PLO65_16675"
     CDS             1515..>1743
                     /locus_tag="PLO65_16675"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD29933.1"
                     /translation="MKFEDYQRFTFRRDGRILVCSFSGNAVNAVDARMHDELAELFVD
                     LQHDDDSDLIVLTGENRAFCAGGDFDWFDEQI"
BASE COUNT          259 a          591 c          624 g          269 t
ORIGIN      
        1 ccccggccgc ctgcttcagt ttctggcccc gggtgcgcgt gtcgggcggc ggcggctcgt
       61 cgagatccgg ccccagcgcc tcgtccagcc ggccgatctc gttggcgatg gcttcgcagc
      121 gggtcaggcc atccatgtca taggggccgg cgaccgcgcg cttcagcacg tcgggaatgg
      181 ccgtgtgctt gagattcagg tcgtcgagcg gggcggtgac ggcgtcgcgc aggttcggcc
      241 ggacctgggt ctcgccgcgg tcctggcgcg aatcggggcg atcgccctcc tgcgccaggg
      301 acggcccggc gagaagaagg ctcagggctg tgatcaggct caggcggcgc atacgaggtg
      361 catagtcgat tacgcgccgc ggcggaattg tgaccccgga ggatggcttc ccccccgcca
      421 catgatccgc ctaaagtctt cgccagaccg ggccgacccg gcgcaggagg aacgatgccc
      481 ggaacaaccc gccgctgggt gctggcgcgc catgtcgagg gggcgccgac gcccgacgac
      541 ttccggctcg aagagacgcc gctcggcgaa ctggccgacg ggaagttcct ctcccgcgtg
      601 atcctcggct cggtcgaccc cggcacgcgc tcgcgcctgt ccggcggcga cagctacgcc
      661 ggggcgatga agatcggcga gccgatggac ggcttctgcg tggcgcaggt catcgagagc
      721 agacacccca acttcgccgt cggcgacctg atcgcggccg gcggcggctg gcgggagcat
      781 ttcgtgtcgg acggcagggg cttcatccag aagatcaccg accgccgcgt gccgctgggc
      841 tgctggatcg gcgtcctcgg ggtgccgggc ctgaccgcct ggttcggtct caaccgcgtg
      901 ggccaggcga aggaaggcga gaccctgctg gtcacctccg ccgccggccc ggtcggcgca
      961 acggccggcc agatcggcaa gaagctcggc ctgcgcgtcg tcggcgtcgc cggcgggccc
     1021 cggaagtgcg cctggctgca ggacgaggcc cgcttcgatg cggtcatcga ctacaaggcc
     1081 gagccggacc tgaccgccgc catcaaggcg gcctgtccgg aaggggtcga cgtgctgttc
     1141 gacaacgtcg gcaatgaaat ggtcgatcgc gtcctgccgc tgatgaagct gcgcgggcgg
     1201 gtcgtcgtct cgggccaggt ggccgactac aatgttccgg tcgagcagcg gtacggcctc
     1261 aagcacaccg acgtcttcat cacccaccgg gtgcgcatgg aggggctggt ggtgttcgac
     1321 gacatccgcc agttcgccgc cgcccagagc cagatggccg actggatcgc cgacggctcg
     1381 ctgaagttcg ccatcgagga gttcgacggc ctggagcgcg cggccgaggc cttctgcggc
     1441 ctgttccgcg gcgagaattt cggccgccgg ctggtacgcg tcagcccaga tccgtcttgg
     1501 gaggatcccg cctgatgaag ttcgaggact accagcgctt caccttccgc cgcgacggca
     1561 ggatcctggt ctgcagcttc agcggcaacg cggtgaacgc cgtcgacgcc cgcatgcacg
     1621 acgagctggc cgagctgttc gtcgacctgc agcatgacga cgacagcgac ctgatcgtgc
     1681 tgaccggcga gaaccgcgcc ttctgcgccg gcggcgactt cgactggttc gacgagcaga
     1741 tca
//
LOCUS       DAORNX010000562         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49236_length_1743_cov_4.366114, whole genome shotgun sequence.
ACCESSION   DAORNX010000562 DAORNX010000000
VERSION     DAORNX010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49236_length_1743_cov_4.366114"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1510)
                     /locus_tag="PLO65_16680"
     CDS             complement(134..1510)
                     /locus_tag="PLO65_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRD29934.1"
                     /translation="MTEVLLTAPEGETIPVAVVAEGDLAGLSPFEQGLAATAQFKGKA
                     GQVLAAPGPEGRTARVLFGLGDPGKAEAMVFRGLAARLTAGDYRLEGLPAGLDPTQVA
                     TAFALGSYRFDRYRKTRDGQPRLVAPDGTDVAEAREIAHACALARDMVNTPANDMGPL
                     QIETIAREIAARHGATISVLTGDALLEQNYPSVHAVGRAADPARAPRIIELGWGRPGH
                     PLVALVGKGVVFDTGGLDIKPSSGMRLMKKDMGGAAHVLALARMIMAAELPVRLAVLV
                     PAVENAISGDAMRPGDVLATRKGLTVEVGNTDAEGRLILSDALTRAAELEPALTIDMA
                     TLTGAARVALGPQVIPFFTDDEDLAGEIGAASRAVADPLWRLPLWPGYEEALDGDVAD
                     LKNDPDGWAQAGAVTAALFLKRFAPTGPWVHLDIFAWNPKGRPGYPVGGEAQAVRALF
                     AMIRARFR"
BASE COUNT          234 a          640 c          642 g          227 t
ORIGIN      
        1 cgaccgccgg agagaccccg gcagccgcgc ccgcgacgcc gccggcgaag taggtcccgg
       61 cccggaccga tgaccagaag gccgcggcgg ctcaccgacg cggccttttt catgccttgc
      121 aacgacggcg cgcctaccgg aaccgggccc tgatcatggc gaacagcgcg cgaacggcct
      181 gggcctcgcc gccgaccgga tagccgggcc ggcccttcgg gttccaggcg aagatgtcga
      241 ggtggaccca cgggcccgtc ggcgcgaacc gcttgaggaa cagggccgcg gtcaccgcgc
      301 cggcctgggc ccagccgtcg ggatcgttct tcaggtcggc cacatccccg tccagcgcct
      361 cttcgtaacc gggccacagg ggcagccgcc agagcgggtc ggccaccgcg cgcgaggcgg
      421 cgccgatctc gccggccagg tcctcgtcgt cggtgaagaa cggaatcacc tgcggcccca
      481 gggcgacacg ggcggcgccg gtcagggtgg ccatgtcgat ggtcagggcc ggctccagtt
      541 cggccgcgcg ggtcagggcg tccgacagga tcagccggcc ctcggcgtcg gtgttgccga
      601 cctcgacggt gaggcccttg cgcgtggcca gcacgtcgcc gggccgcatg gcgtcgccac
      661 tgatcgcgtt ctccaccgcc ggaaccagca cggccagccg caccggaagc tcggcggcca
      721 tgatcatccg ggccagggcc agcacgtggg cggcgccgcc catgtccttc ttcatcagcc
      781 gcatgccgga ggacggcttg atgtccaggc cgccggtgtc gaacaccacg cccttgccga
      841 ccagcgccac cagcggatgg ccggggcggc cccagcccag ctcaatgatg cgcggggcgc
      901 gggcgggatc ggccgcccgg ccgacggcgt gaacggaggg gtagttctgc tcgagcaggg
      961 cgtcgccggt gagaacggag atcgtcgcgc catgccgcgc ggcgatctcg cgcgcgatgg
     1021 tctcgatctg cagcggcccc atgtcgttgg ccggggtgtt gaccatgtcg cgggccaggg
     1081 cgcaggcatg ggcgatctcg cgcgcctcgg cgacatcggt cccgtcgggc gcgaccagcc
     1141 ggggctggcc gtcgcgcgtc ttgcgatagc ggtcgaaacg gtagctcccg agggcgaagg
     1201 cggtggcgac ctgggtcggg tcgagaccgg cgggcagccc ctccagccgg tagtcgccgg
     1261 cggtcagccg cgcggccagc ccccggaaca ccatggcttc ggccttgccc ggatcgccca
     1321 ggccgaacag caccctggcg gtcctgccct ccgggcccgg cgcggccagg acctggccgg
     1381 ccttgccctt gaactgggcg gtcgcggcca gtccctgttc gaacggcgac aggccggcga
     1441 ggtcgccctc ggcgacgacg gcgacgggaa tggtctcgcc ctcgggggcg gtgagcagga
     1501 cttcggtcat ggggcgccga tttatgctta acgattcctt gagccggacc ggcgaggctg
     1561 cgagccagac gtcgccagag gctcgccttt catgtgtcgc atgccggccc tcgccgcaac
     1621 cattctgacc ctcgccggtg ttccggccat ggcggcgaca ccggcctcgc cgggcgacgc
     1681 gccgatgacc gccgcgccgg cggccccggt cccgcgggtc aaggccactg ccgcccagcg
     1741 ggc
//
LOCUS       DAORNX010000563         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49308_length_1741_cov_5.488138, whole genome shotgun sequence.
ACCESSION   DAORNX010000563 DAORNX010000000
VERSION     DAORNX010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49308_length_1741_cov_5.488138"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>268)
                     /locus_tag="PLO65_16685"
     CDS             complement(<1..>268)
                     /locus_tag="PLO65_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRD29935.1"
                     /translation="RHSITAADGGLLFAAGLWEDSTPDEGPLVSYTMVMAATAPGDDM
                     APFHDRQPVLLDAGRAATWLDLTADYAPILRALPPRTLAFDPPEP"
     assembly_gap    269..338
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<339..656)
                     /locus_tag="PLO65_16690"
     CDS             complement(<339..656)
                     /locus_tag="PLO65_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29936.1"
                     /translation="MCNAYNLKTRLHSVGEALRGQLGLDLALPGGITAETSNLPVPEA
                     VYPKKDGLFLRRAFDAGSSGLEPVIGRWGLVPFNHRGPLKAVKFPCNNARSETMAGKW
                     PFKE"
     gene            complement(688..897)
                     /locus_tag="PLO65_16695"
     CDS             complement(688..897)
                     /locus_tag="PLO65_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29937.1"
                     /translation="MARMFEILRTAMPGAGDTLKIECGACKHRATWSRRRAFDTFGEG
                     AAPYDVGKGLVCSRCRETRNLRVWV"
     gene            1521..1724
                     /locus_tag="PLO65_16700"
     CDS             1521..1724
                     /locus_tag="PLO65_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29938.1"
                     /translation="MVKPRDARPRPVPLTPEEAAALDAAYDDLQARLDRYWALRRPAD
                     SADAIDRARGPGEEDGLAAGRRS"
BASE COUNT          231 a          618 c          591 g          231 t
ORIGIN      
        1 ggctccggcg ggtcgaacgc cagcgtcctc ggcggcaacg cgcgcaggat cggcgcgtag
       61 tccgcggtca ggtcgagcca ggtcgcggcg cggccggcgt cgagcagcac cggctggcgg
      121 tcgtggaacg gggccatgtc gtcgcctggg gcggtggcgg ccatgaccat ggtgtagctg
      181 accagcggcc cctcatcggg cgtgctgtcc tcccacaggc cggccgcgaa cagcaggccg
      241 ccgtcggcgg cggtgatgct gtggcgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ccttgaacgg ccatttgccg
      361 gccatggtct cggagcgggc gttgttgcag gggaacttga ccgccttgag cgggcccctg
      421 tgattgaacg gcaccagccc ccagcggccg atcaccggct ccaggccgct gctgccggcg
      481 tcgaaggcgc gccgcaggaa cagcccgtcc ttcttcggat agacggcctc cggaacgggc
      541 aggttggagg tctcggccgt gatcccccct ggcagcgcca gatcgaggcc cagctggccg
      601 cgcagggcct cgccgacgct gtgaaggcgg gtcttcagat tgtaggcgtt gcacatggcg
      661 aaaggctagg accgcgcccg gggggcgtca aacccagacc ctgaggttgc gggtttcgcg
      721 gcagcggctg cagaccaggc ccttgcccac gtcgtagggc gcggccccct cgccaaaggt
      781 gtcgaacgcc cgcctgcggc tccaggtcgc ccggtgcttg caggcgccgc actcgatctt
      841 cagcgtgtcc ccggcgcccg gcatggcggt gcgcaggatc tcgaacatcc gcgccattca
      901 ccgcccatgc ttcgacagcc gccccggcgc cagcatggcc atggccaggt cgcggtcgtc
      961 cggggcgggg ttttgcatgt tgccattttg ttctggccgc ctgccgggag tcaacgccga
     1021 tcgccccggg ggcgaagacc ttcctcgccc gccaaggaca ggaaggtcgg aggcccttcc
     1081 gccgatccct gcgtccaacc ctttgaaatc cctcaactcc gtccgcgtaa ccccgccgcg
     1141 ctggacaatc ttcccctcgc ccctccccac cacccccata ctgctccctg tcccgccgca
     1201 cggggagggc gccaaggcca ggcgatccga ggcggcggcg ggacggggtt cgagcgggga
     1261 caggtgagcg ggggttacgc tcacggtccg gggacggctt cgccgccgcc cgcccgccgg
     1321 aggcttctgc cggctaagcg ttaaaggcgc tgcccaccgg cggcccccgg aaaacggacc
     1381 tcgcggcgcg agacggactt tcccttctca cacgctcaca atccagcatc cgggtgagac
     1441 cggagcgcgc cgcgccttcc ccgccttcac ccggcccggc ccgtgaagcg gcgccgccgc
     1501 gcgccccgat ggagacgccc ttggtcaaac ctcgcgacgc ccgccccagg cccgtccccc
     1561 tgacgccgga agaggcggcg gcgctcgacg cggcctatga cgacctgcag gcgcggctcg
     1621 accgctactg ggcgctcagg cgcccggccg acagcgccga cgccattgac cgggcccgcg
     1681 gacccggcga ggaagacggc ctggcggcgg ggaggcggtc ctgatggaaa cggcggcggg
     1741 g
//
LOCUS       DAORNX010000564         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49426_length_1738_cov_9.301248, whole genome shotgun sequence.
ACCESSION   DAORNX010000564 DAORNX010000000
VERSION     DAORNX010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49426_length_1738_cov_9.301248"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLO65_16705"
     CDS             <1..304
                     /locus_tag="PLO65_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRD29939.1"
                     /translation="FVGYYVVWRVPPALHTPLMAVTNAISSVIIVGALLAAAAHGAGG
                     DLAGGGAIPGNVWISKGAGALAAAFAAVNIFGGFLVTQRMLGMYKKKEPAKGKSGH"
     gene            327..>1738
                     /locus_tag="PLO65_16710"
     CDS             327..>1738
                     /locus_tag="PLO65_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRD29940.1"
                     /translation="MNANFAAIAYIVSGVLFILALRGLSSPETSQAGNRNGMIGMAIA
                     VGVTLLTLWGQNKLDPVTLLLIGVGVAIGGGVGAVIARRVAMTSMPQLVAAFHSLVGM
                     AACLVAVAAIYTPDAYGIAEAGRIHLQSLIELSVGLAIGAITFTGSVIAFAKLNGNMS
                     GAPILLPARHLLNLAIAAAIVVLTVILVMSGGAAIWAFWAIFALSLLIGVTLIIPIGG
                     ADMPVVVSMLNSYSGWAAAALGFTLQNVTLIITGALVGSSGAILSYIMCKGMNRSFIS
                     VILGGFGGEDAAAAAGGKVETRPVKQGSAEDAAFIMKNASKVIIVPGYGMAVSQAQHA
                     LREMADKLKEEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINAEFPTAD
                     VAFVIGANDVTNPAAKTDPTSAIYGMPILDVEKARTVLFVKRGMSSGYAGVENELFFR
                     DNTMMLFADAKKMVEGIVKNL"
BASE COUNT          281 a          605 c          571 g          281 t
ORIGIN      
        1 cttcgtcggc tactatgtgg tctggcgcgt gccgcccgcc ctgcatacgc cgctgatggc
       61 cgtgaccaac gccatctcct cggtgatcat cgtcggcgcc ctgctggccg ccgccgcgca
      121 cggagcgggc ggtgatctgg ccggcggcgg ggcgatcccc ggcaacgtct ggatctccaa
      181 gggcgccggc gccctggccg ccgccttcgc cgcggtgaac atcttcggcg gcttcctcgt
      241 cacccagcgg atgctgggca tgtacaagaa gaaagagccc gccaagggca agagcggcca
      301 ctaggcgcgt tggggaacat cagatcatga acgccaactt cgccgccatc gcctacatcg
      361 tctcgggcgt gctgttcatt ctggcgctgc gggggctgtc gagccccgag accagccagg
      421 cgggcaaccg caacggcatg atcggcatgg ccatcgccgt cggcgtgacc ctgctgacgc
      481 tgtggggcca gaacaagctc gatccggtca ccctgctgct gatcggcgtg ggcgtggcca
      541 tcggcggcgg ggtcggggcg gtgatcgccc gccgcgtggc catgacctcg atgccgcagc
      601 tggtcgccgc cttccacagc ctggtcggca tggccgcctg cctggtcgcc gtggccgcca
      661 tctacacgcc ggacgcctat ggcatcgccg aggctggccg catccacctg cagtcgctga
      721 tcgagctgtc ggtgggtctg gccatcggcg ccatcacctt caccggctcg gtgatcgcct
      781 tcgccaagct caacggcaac atgagcggcg cgccgatcct gctgcccgcc cgccacctgc
      841 tgaacctggc catcgccgcg gccatcgtgg tgctgacggt catcctggtg atgagcggcg
      901 gcgcggccat ctgggccttc tgggcgatct tcgccctcag cctgctgatc ggcgtgaccc
      961 tgatcatccc catcggcggc gccgacatgc cggtcgtggt gtcgatgctc aacagctaca
     1021 gcggctgggc cgccgcggcc ctgggcttca ccctgcagaa cgtcaccctg atcatcaccg
     1081 gcgccctggt cggcagctcg ggcgcgatcc tcagctacat catgtgcaag gggatgaacc
     1141 gcagcttcat ctcggtgatc ctgggcggct tcggcggtga agacgccgcc gctgcagcag
     1201 gcggcaaggt cgagacccgt ccggtcaagc agggcagcgc cgaggatgcc gccttcatca
     1261 tgaagaacgc cagcaaggtg atcatcgtcc ccggctacgg catggccgtc agccaggcgc
     1321 agcacgccct gcgcgaaatg gccgacaagc tgaaggaaga gggcgtggag gtgaagtacg
     1381 ccatccaccc cgtcgccggc cgcatgccgg gccacatgaa cgtgctgctg gccgaggcca
     1441 acgtccccta tgacgaggtc ttcgagcttg aggacatcaa cgccgagttc ccgaccgccg
     1501 acgtggcctt cgtcatcggg gccaacgacg tgaccaaccc ggccgccaag accgacccga
     1561 ccagcgccat ctacggcatg ccgatcctcg acgtggagaa ggcgcgcacc gtgctgttcg
     1621 tgaagcgcgg catgtcgagc ggctacgcgg gcgtcgagaa cgaactgttc ttccgcgaca
     1681 acaccatgat gctgttcgcc gacgccaaga agatggtcga ggggatcgtg aagaacct
//
LOCUS       DAORNX010000565         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49430_length_1738_cov_4.387403, whole genome shotgun sequence.
ACCESSION   DAORNX010000565 DAORNX010000000
VERSION     DAORNX010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49430_length_1738_cov_4.387403"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLO65_16715"
     CDS             complement(<1..345)
                     /locus_tag="PLO65_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD29941.1"
                     /translation="MNGADALITTLADNGVTACFANPGTSEMQFVSALDREPRMRSVL
                     CLFEGVATGAADGYGRMAGRPACTLLHLGAGYANGGANLHNARRAGTPVVNVIGDHAT
                     YHAKYDAPLQSDI"
     gene            complement(419..>1738)
                     /locus_tag="PLO65_16720"
     CDS             complement(419..>1738)
                     /locus_tag="PLO65_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD29942.1"
                     /translation="ERPRGPWTRLGEPLAILRPVSTREVSTILRLVSAAGASVTPWGG
                     CTGLVDGCLADNTLALSLERMAAIESIDATSGTMTVQAGCILQTACEAAEAAGLFLPL
                     DLGARGSATIGGNISTNAGGNRVLRYGMTRDMVLGLEVVLADGTVIPALRSLMKNNTG
                     YDLRQLFIGAEGTLGVVTRAVLRLRPAPVSQDTAFVGVGSFDGVARLLRRLERRLGGA
                     MSAFEVMWADFHDLVTSPPARGRAPLEGRHPFYVLIESLGGDEAADAARFEAALTEAL
                     EDGDIDDAVIARSGAERAAMWALRDDVGQTSREGPIIAFDVSLPIAAMEAYVDGVCAD
                     YAGRWPELGRPWVFGHLGDGNLHILVRIPSGAGLAERHAIEAMVYEPLQAIGGSISAE
                     HGIGLAKRDWLPLSRAPEEIALMARLKHALDPAGVLNPGKILAPPAP"
BASE COUNT          240 a          652 c          607 g          239 t
ORIGIN      
        1 gatgtcgctc tgcagcggag cgtcgtactt ggcgtggtag gtggcgtggt cgccgatcac
       61 gttgaccacg ggcgtcccag cgcggcgggc gttgtgcagg ttggcgccgc cgttggcgta
      121 gccggcgccc aggtgcagca gggtgcaggc cggcctgccg gccatccggc cgtagccgtc
      181 cgccgcgccg gtcgccacgc cctcgaacag gcagagcacc gagcgcatgc gcggctcgcg
      241 gtccagcgcg gagacgaact gcatctcgct ggtgccggga ttggcgaaac aggcggtcac
      301 gccgttgtcg gcgagggtgg tgatcagggc gtcggcgccg ttcatggtta tctcccgtag
      361 gggcgcgcgc cccgcctccc catccaacat ttcgtcgccc gcgccaaccg gctgcgggtc
      421 agggcgcggg cggcgcgagg atttttccgg ggttgagcac ccccgccgga tcgagcgcgt
      481 gcttgagccg ggccatcagt gcgatctcct cgggcgcgcg cgacaacggc aaccagtcgc
      541 gcttggccag gccgataccg tgctcggccg agatcgatcc gccgatcgcc tgcagcggct
      601 catagaccat cgcctcgatc gcatggcgct cggccaggcc cgcgccggac gggatgcgca
      661 cgaggatgtg caggttgccg tcgcccaggt ggccgaacac ccacggccgc ccgagctcgg
      721 gccagcgccc cgcatagtcg gcgcacacgc catccacata ggcctccatc gcggcgatcg
      781 gcaggctgac gtcgaaggcg atgatcggcc cctcgcggct ggtctggccg acatcgtcgc
      841 gcagggccca catggccgcc cgttcggccc cggaccgggc gatgaccgcg tcgtcgatgt
      901 cgccgtcctc gagggcttcg gtcagggccg cctcgaaacg cgcggcgtcg gccgcctcgt
      961 cgccgccgag gctctcgatc aggacgtaga agggatggcg tccctcgagc ggcgcccggc
     1021 cccgcgccgg cggactggtc accaggtcgt ggaagtcggc ccacatcacc tcgaaggccg
     1081 acatcgcccc gcccagccgt cgttcaagcc gccgcagcag ccgcgccacc ccgtcgaacg
     1141 aaccgacgcc gacgaaggcc gtgtcctggc tgaccggcgc gggccgcagg cgcagcaccg
     1201 cgcgcgtcac cacacccagc gtgccctccg cgccgatgaa cagctggcgc aggtcgtagc
     1261 cggtgttgtt cttcatcagc gatcgcaggg ccgggattac cgtcccgtcg gccagcacca
     1321 cctcgagacc cagcaccatg tcgcgggtca tgccgtagcg cagcacccgg ttgccgccgg
     1381 cgttggtaga gatgttgccg ccgatcgtcg ccgaaccgcg cgcgcccagg tccagcggca
     1441 ggaacagtcc ggccgcctcg gccgcctcgc aggcggtctg caggatgcag cccgcctgca
     1501 cggtcatggt cccgctcgtg gcgtcgatgc tctcgatggc cgccatccgc tccagcgaca
     1561 gggccagggt gttgtcggcc aggcaaccgt cgaccaggcc cgtgcagccg ccccagggcg
     1621 tcaccgacgc gcccgccgcg ctgaccagcc tgaggatcgt cgacacctcc cgcgtcgaga
     1681 ccggccgcag gatcgccagc ggctcgccga gccgcgtcca gggcccgcgc ggccgttc
//
LOCUS       DAORNX010000566         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49463_length_1737_cov_6.130202, whole genome shotgun sequence.
ACCESSION   DAORNX010000566 DAORNX010000000
VERSION     DAORNX010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49463_length_1737_cov_6.130202"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLO65_16725"
     CDS             complement(<1..450)
                     /locus_tag="PLO65_16725"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRD29943.1"
                     /translation="MEDDRGRAGNGDGQHQPDSEARGIGRAAAALRGGDLVILPTETV
                     YGLAANAADPRAVAAVYAAKGRPAFNPLIAHVADLEAARRVAVLDDRAEALAATFWPG
                     PLTIVAPVRDETAVCDLARAGLDTVAVRVPGHPLCRAVLRAFGGPLVA"
     gene            complement(378..944)
                     /locus_tag="PLO65_16730"
     CDS             complement(378..944)
                     /locus_tag="PLO65_16730"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRD29944.1"
                     /translation="MRPFVPVLVIAATLAAPAWAAPAPAWTVDKAASKVAFASSFDGG
                     AFSGSFRRWDAAIRFDPANLAGSSVTATFDMTSAATGDASRDEALPLDDWFAAGRFPK
                     ATFTARTFKALGGDRYQAVGDLTLRGVTKPLTLPFTLVITGGVARMNASVGLNRLAFG
                     VGQNEWKTTEVVPGTVTVNISLTAKRAG"
     gene            complement(941..1528)
                     /locus_tag="PLO65_16735"
     CDS             complement(941..1528)
                     /locus_tag="PLO65_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRD29945.1"
                     /translation="MTQSRYSTVAILLHWAIAALLVWAVLLAWQSEDLKGAAKIAVLQ
                     LHKPIGITIMILTLVRIAWRFVKPAPVMGDHLKPWERQLARGVHLLFYALLLIIPLTG
                     WAMVSASALIHQYPINMFGLFNWPVMTPLFELPAELRHARHEQLEDIHKLLAKVVIYG
                     LIPLHILGALKHQFLDRDNELAKMIPFLARKDVAA"
     gene            complement(1554..>1737)
                     /locus_tag="PLO65_16740"
     CDS             complement(1554..>1737)
                     /locus_tag="PLO65_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRD29946.1"
                     /translation="VTLDVIFNGYASGMAGQRSGFSARGVIKRSEFGSTFLLNPPLAF
                     VGDEVELILELEFVRK"
BASE COUNT          294 a          574 c          623 g          246 t
ORIGIN      
        1 cgccaccagc gggccgccga acgcccgcag caccgcgcgg cacagggggt gccccggaac
       61 ccgtacggcc acggtgtcga gccccgccct ggccagatcg cagaccgcgg tctcatcgcg
      121 caccggcgcg acgatcgtca gggggccggg ccagaaagtc gctgcaaggg cttcggcgcg
      181 atcatcaagc acggcgacgc gccgcgcagc ctcgagatcg gcgacatggg cgatcagcgg
      241 attgaaggcc ggccggccct tggccgcgta gacggccgcc acggcccgcg gatcggcggc
      301 gttggcggca agaccgtaga cggtctccgt cggcaggatg acgaggtcgc cgccgcgcag
      361 ggccgcggcc gcgcggccta tcccgcgcgc ttcgctgtca ggctgatgtt gaccgtcacc
      421 gttcccggca cgacctcggt cgtcttccac tcgttctgtc ccacgccgaa ggccagacgg
      481 ttcaggccca cgctggcgtt catcctggcc acgccgccgg tgatgacgag ggtgaagggc
      541 agggtcaacg gtttcgtcac gccgcgcagc gtcagatcgc cgacagcctg gtagcggtcg
      601 ccgcccagcg ccttgaaggt tctcgcggtg aaggtggcct tcgggaaccg gccggccgcg
      661 aaccagtcgt ccagcggcag ggcctcgtcg cggctggcgt cgccggtggc ggccgacgtc
      721 atgtcgaagg tcgcggtgac cgacgagccg gccaggttgg ccggatcgaa gcggatggcc
      781 gcatcccagc gccggaacga tccgctgaag gcgccgccgt cgaaactcga ggcgaaggcc
      841 accttcgagg cggccttgtc caccgtccag gccggcgcgg gggccgccca ggccggagcg
      901 gccagggtgg cggcgatgac caggacgggg acaaagggtc tcacgcggcg acgtccttgc
      961 gggccaggaa cgggatcatc ttggccagct cgttgtccct gtcgaggaac tggtgcttca
     1021 gcgcgcccag gatgtgcagc gggatcaggc cgtagatgac caccttggcc agaagcttgt
     1081 ggatgtcctc cagctgctcg tggcgggcgt gccgcagctc ggcgggcagc tcgaacagcg
     1141 gggtcatgac cggccagttg aacaggccga acatgttgat cggatactgg tggatcaggg
     1201 cgctggccga aaccatcgcc cagccggtca gcgggatgat gagcagcagg gcgtagaaca
     1261 gcaggtggac gccgcgggcc agctgtcgct cccagggctt caggtggtcg cccatcaccg
     1321 gcgccggctt gacgaagcgc caggcgatgc ggaccagggt cagtatcatg atggtgatgc
     1381 cgatcggctt gtgcagctgc aggacggcga tcttggccgc gcccttcaag tcctcggact
     1441 gccaggccag aagaacggcc cagaccagca gggcggcaat ggcccagtgc agcaggatgg
     1501 cgacggtcga atagcgggac tgggtcatgg gcgctccggg tttcgacctc agcctacttc
     1561 ctgacgaact caagttcgag gatcaactcg acctcgtcgc cgacaaaggc cagcggcggg
     1621 ttgagcagaa aggttgatcc gaactcggac cgcttgatca cgccccgcgc cgaaaatccc
     1681 gagcgctggc cggccatgcc cgaggcgtag ccgttgaaga tcacgtcgag cgtcacc
//
LOCUS       DAORNX010000567         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_49934_length_1723_cov_6.711631, whole genome shotgun sequence.
ACCESSION   DAORNX010000567 DAORNX010000000
VERSION     DAORNX010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49934_length_1723_cov_6.711631"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1296)
                     /locus_tag="PLO65_16745"
     CDS             complement(289..1296)
                     /locus_tag="PLO65_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29947.1"
                     /translation="MTRFGRLFTLAGAVLAVSIASYAAPASAQATRTWVSGVGDDANP
                     CSRTAPCKTFAGAISKTAAGGEIDTLDPGGFGSVTITKSITLDGSGGSTAGVLVGGTN
                     GFVINDGGAGTVVVTIRNMDLEGIGFSSGSAGIRGIWFVSGASLTVENSTIRNFRDPT
                     NGTGIMFTPSGAGELYVNNVTLSGNGNGTSGGGILIKPTGTGSARAVLSNVRARDNNT
                     GFGLKLDTTGNTGTGINAMVTSSDFSGGGIGVAVVTPPSTTSATLMLTNSTVSQNTNF
                     GIISNGATATVRVGNTVVTGNGTGLLAGGTGVLSTYGDNMVDGNTTNGVFSPSTAITK
                     K"
     gene            1438..>1723
                     /locus_tag="PLO65_16750"
     CDS             1438..>1723
                     /locus_tag="PLO65_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29948.1"
                     /translation="MPRSHRSGFSVLEAIIVLAISGTALLLVFEMGVSGSQRGFRLGN
                     QALSAADAEPGAGSLRAIIRGARFDSAGRSTAGARGPVGDARVFSTPVSVD"
BASE COUNT          247 a          592 c          591 g          293 t
ORIGIN      
        1 cccgcgccag gccgtaggcg cggtccgccg ggccgttcgc cagggccagg gccgagagcg
       61 ccgccagctt gcgtgcgtcg ccgcgggcct gaagcacgag gggttcgaac gccgcgcggg
      121 ccttcgccgc cagtttcccc tcaaccgtca tcgcaccctc cgcctgccgc ccgtcctcct
      181 tgccacaccg gacctcgaca gcccaaggcg cgagatgcgg atcgcggaca cgaaaaaggg
      241 cgggcgggac cgtccggtcc cggcccgccc tccccgtccg agatcgccct atttcttggt
      301 gatcgccgtg gatggcgaga acacgccatt ggtcgtgttt ccgtcgacca tgttgtcgcc
      361 gtaggtgctg agcacgcccg tgccgccggc gagcaggccc gtgccgttgc cggtcaccac
      421 ggtattgccc acgcgaacgg tcgcggtcgc gccgttggag atgatgccga agttcgtgtt
      481 ctggctgacc gtcgagttgg tcagcatcag ggtcgcgctg gtggtgctgg gcggggtgac
      541 cacggcgacg ccgatgccgc cgccgctgaa gtcactggag gtgaccatgg cgttgatgcc
      601 cgtgccggtg tttccggtcg tgtcgagctt gagaccgaag ccggtattgt tgtcgcgcgc
      661 ccgcacgttg gacaggaccg ccctggcgct gccggtcccg gtcggcttga tgaggatccc
      721 gccaccgctc gtgccgttgc cgttgcccga cagggtgacg ttgttgacgt agagctcgcc
      781 cgcgccgctc ggggtgaaca tgatgcccgt gccgttggtc gggtcccgga agttgcggat
      841 ggtcgagttc tcgaccgtca gcgaggcgcc ggacacgaac cagatgccac ggataccggc
      901 cgacccgctg gagaagccga tgccctccag atccatgttg cggatggtga caaccaccgt
      961 tccagcgccg ccgtcgttga tgacgaagcc gttcgtgccg cccaccagaa cgccggcggt
     1021 gctgccgcca ctgccgtcca gggtgatcga cttggtgatc gtcacggagc cgaagccgcc
     1081 cggatcgagc gtgtcgatct cgccgccggc cgccgtcttg gagatggcgc ccgcgaatgt
     1141 cttgcagggc gccgtgcggc tgcagggatt ggcgtcgtcg ccaacgccgg aaacccaggt
     1201 gcgagtcgcc tgggccgatg ccggcgcggc gtagctcgcg atcgaaacag cgagcaccgc
     1261 gcccgccagc gtaaacagtc ttccaaaccg agtcattggc gccctcccag gcttgcatat
     1321 gcagcgaacc agactgcatt tgtcgccgac attggcacgc agccgggcct tgcgatactc
     1381 gtgttttgcg atcacgcccc catccagcga ccggcgacat cgaacagagg actgcccttg
     1441 cccaggagcc atcggtcggg attctcggtg ctcgaggcga tcatcgtcct ggccatatcc
     1501 ggcacggccc tgctgctggt tttcgagatg ggggtctccg gatcgcagag gggctttcgg
     1561 ctgggcaacc aggcgctttc cgccgccgac gccgagccgg gcgccggtag cctgcgcgcc
     1621 ataatccggg gcgcgcgctt cgattccgcc gggcgatcga cggccggcgc ccggggtccg
     1681 gtcggcgacg cgcgcgtctt cagcacgccg gtgagcgtcg aca
//
LOCUS       DAORNX010000568         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_50358_length_1710_cov_5.912991, whole genome shotgun sequence.
ACCESSION   DAORNX010000568 DAORNX010000000
VERSION     DAORNX010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50358_length_1710_cov_5.912991"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..551
                     /locus_tag="PLO65_16755"
     CDS             120..551
                     /locus_tag="PLO65_16755"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRD29949.1"
                     /translation="MTARRAGFSLIEAMAALLIVSLVLLAALSLQGQMAQAERRYGRA
                     ITVSELRRTAMVLVRDINPAVEPGGTRPLAGGRRLAWSAVPVNAFRPSTDGRFEVRLY
                     RVTTTLFEPDGRVLSTQTYQRVGWRPRQRGAATGAGSMRRP"
     gene            complement(506..>1710)
                     /locus_tag="PLO65_16760"
     CDS             complement(506..>1710)
                     /locus_tag="PLO65_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRD29950.1"
                     /translation="ALQSARTWIGRLDRAEALGGRTTTFVYQVRNTDAASVAALISAA
                     RGEDQQRTPADTGQPGQPPQDTASQNDSRGLAASAFGDGRMAVDESGNRIVFTGSAED
                     FMELRGLMVALDTPRPQVLVEVTVAEVTLTDETRVGLEWFFTRSMSNGQLEGGTKGGL
                     GLDTGGLTLKFTGTDVQAAFNAFASNNKVNILSRPRLVARSGDEARIQVGTDVPIITS
                     RANSPTQTGGSSDILQTIQYRQTGVILTIRPVIYSDGRVDLQITQEVSSQQPNSNTAI
                     GSPLILNRSVTTRLSVDEGATAVIGGLIDDNYTKGNSGIPLLKDIPLLGAPFRVDSFS
                     TGKTELIILVTPHILRDGGEMAFWTQRSTADMNAAFAVGRGWSYTLTPFTAGQGLRID
                     PAPVAAPR"
BASE COUNT          218 a          582 c          624 g          286 t
ORIGIN      
        1 gccgtcatcg gcatcctcct gatgctgttc ggaagggccg cggcgagcgg cgtggcgccg
       61 ctgggcgccg ccctggccgc tccggcgctg gtcgcggcct gtctcgcccg gtggccgaca
      121 tgaccgcccg ccgcgccggc ttcagcctga tcgaggcgat ggccgcgctg ctgatcgtct
      181 cgctggtgct gctggccgcc ctgtcgcttc agggccagat ggcccaggcc gagcggcgct
      241 acgggcgggc catcaccgtg tccgaactgc gtcgcacggc gatggtcctc gtgcgggaca
      301 tcaatcccgc cgtcgaaccc ggcgggaccc gaccgctcgc gggaggccgc cggctggcct
      361 ggtcggcggt tccggtcaac gccttccggc cttccaccga cggccgtttc gaggtgcggc
      421 tctatcgggt cacgacaacg ctgttcgaac ctgacgggcg agtcctgtcg acgcagacct
      481 accagcgggt cggctggcgg ccgcgtcagc ggggggcggc gaccggggcc gggtcgatgc
      541 gcaggccctg acccgccgtg aacggcgtca gggtgtagga ccagccccgc ccgaccgcga
      601 aggccgcgtt catgtccgcc gtcgatcgct gcgtccagaa ggccatctcg ccgccgtccc
      661 gcaggatgtg gggcgtgacg aggatgatca gctcggtctt cccggtcgag aaactgtcga
      721 ccctgaacgg cgcgccgagc agcgggatgt ccttgagaag cgggatgccg gaattgccct
      781 tggtgtagtt gtcgtcgatc agaccaccga tcaccgccgt ggcgccttcg tcgaccgaca
      841 gccgggtcgt cacgctgcga ttgaggatca gggggctgcc gatggcggtg ttgctgttgg
      901 gctgctggct cgacacctcc tgggtgatct gcaggtcgac gcggccgtcg ctgtagatca
      961 ccggccggat cgtcaggatc acgccggtct gacggtactg gatggtctgc aggatgtcgg
     1021 aactgccgcc cgtctgggtc ggactgttgg ctcgcgaggt gatgatcggc acgtcggtgc
     1081 cgacctggat ccgggcctcg tcgccgcttc gcgccacgag ccggggccgc gacaggatgt
     1141 tgaccttgtt gttcgaggcg aaggcgttga acgccgcctg gacgtcggtc ccggtgaact
     1201 tcaacgtcag tccgccggtg tcgaggccaa ggccgccttt ggttccgccc tcgagctggc
     1261 cgttcgacat cgaccgggtg aagaaccact ccaggccgac acgggtctcg tcggtcaggg
     1321 tgacctcggc gacggtgacc tccacaagca cctgcggacg tggcgtgtcg agggcgacca
     1381 tcaggccgcg cagctccatg aaatcctcgg ccgagccggt gaagacgatc cggttgcccg
     1441 actcgtccac ggccatccgg ccgtcgccga aggcgctggc ggcgagtccg cggctgtcgt
     1501 tctggctggc cgtgtcctgc ggcggctggc ccggctggcc ggtgtcggcc ggagtccgct
     1561 gctggtcctc gccgcgcgcg gccgagatca gcgcggccac cgacgccgcg tccgtgttgc
     1621 ggacctggta gacgaaggtg gtcgtgcggc cgcccagggc ctcggcgcga tcaagccggc
     1681 cgatccaggt gcgggcgctc tgcagcgccg
//
LOCUS       DAORNX010000569         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_50554_length_1705_cov_5.038788, whole genome shotgun sequence.
ACCESSION   DAORNX010000569 DAORNX010000000
VERSION     DAORNX010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50554_length_1705_cov_5.038788"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="secG"
                     /locus_tag="PLO65_16765"
     CDS             complement(<1..340)
                     /gene="secG"
                     /locus_tag="PLO65_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRD29951.1"
                     /translation="MLLNILLTIQILVAILLGLAVLLQRSEGGALGMGGGPSGFMTAR
                     GQGDLLTRSTWTLFTIFLVLCIALTILQGRERAGSSVVGRGEIERINPVAPPVAPATA
                     PDTAPSGAAPA"
     gene            456..971
                     /locus_tag="PLO65_16770"
     CDS             456..971
                     /locus_tag="PLO65_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD29952.1"
                     /translation="MILETERLKLTPVTVEDGPLIHPIMADPEVMAHMDSVAIDDPDV
                     VEAFIHGQLVEMANEQAFYWSVRLADGGRYLGSVDLSDIDWRHSRAEIGFILGKDAWG
                     KGYGYEAVQAVLGHAATLGFKRLWARTHVGNAASEALLRKLGFEEEGYLRGHVDREGE
                     RRDCRLWGLLL"
     gene            complement(1056..1480)
                     /locus_tag="PLO65_16775"
                     /pseudo
     CDS             complement(1056..1480)
                     /locus_tag="PLO65_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166924.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
     assembly_gap    1481..1499
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1500..>1705)
                     /locus_tag="PLO65_16780"
     CDS             complement(<1500..>1705)
                     /locus_tag="PLO65_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRD29953.1"
                     /translation="RPPGARVAICPPATLVHRLVEALGGTGVEVGGQDCRAEAAGAFT
                     GDIAAEMLVDAGAGLVILGHSERR"
BASE COUNT          260 a          610 c          589 g          227 t
ORIGIN      
        1 gcgccggggc cgcgccggac ggggccgtgt caggcgccgt cgccggggcg accgggggcg
       61 ccaccgggtt gatccgctcg atctcgccgc ggccgacgac cgaggacccg gcccgctcac
      121 gcccctgcag gatggtcagg gcgatgcaga gcaccaggaa gatggtgaac agggtccagg
      181 tcgagcgggt cagcaggtca ccctggccgc gcgcggtcat gaagcccgac ggccccccgc
      241 ccatgcccag ggcgccgcct tcggaacgct gcagcagcac cgccagcccc agcaggatgg
      301 cgacgagaat ctggatcgtg agcaggatgt tcagcagcat tgcgatcttc ggggtccgtt
      361 cggcgcgcag gcgccctgtc gaaacaagcc ggcccctcta gcaccacgca ggcggccgcg
      421 ccatagctgg gcattgttcg ctcccgcgca agcccatgat tctcgaaacc gaacgcctca
      481 agctgacgcc cgtgaccgtc gaggacgggc cgctgatcca tccgatcatg gccgatcccg
      541 aggtcatggc ccacatggac agcgtggcca tcgacgatcc ggacgtggtc gaggccttca
      601 tccacggcca gctggtcgag atggccaacg agcaggcctt ctactggtcg gtgcggctgg
      661 ccgatggcgg ccggtatctc ggcagcgtcg atctgtccga catcgactgg cgccacagcc
      721 gggccgagat cggcttcatc ctcggcaagg acgcctgggg caagggctat ggatacgagg
      781 ccgtccaggc cgtgctcggc catgccgcga ccctcggctt caagcgactg tgggcccgca
      841 cccatgtcgg caacgccgcc tccgaggccc tgctgcgcaa gctggggttc gaggaagagg
      901 gctatctgcg cggccatgtc gaccgcgagg gcgaacggcg cgactgccgc ctgtggggat
      961 tgctgctgta gcggggacat gtaccgcagg tacgtgtccc ccaatcgagc ggctggcttc
     1021 aggggacacg tacctgcggt acatgtcccc gcgcatcacg ccgcctcgat gatccccaga
     1081 aagtccgccg ccttgaggga ggcgcccccg accagggcgc cgccgacgtc gcgcaggcgc
     1141 aggatttcgg ccgcgttgga cggcttgacc gagccgccat agaggatcgg cgtgcggccg
     1201 ccctggtcgc cgaacatctc gaccagcacc gcgcggatcg ccacgtgcac ggcctcgatc
     1261 tgttcaagag tcggcgtcag gcccgtgccg atcgcccaga ccggctcgta ggcgacggcg
     1321 aacgggctgc cggcgagcac ctcgggcagg gagcccctca cctgccccgt gacgatctcc
     1381 agcgtgcgcc cggcctcgcg ttcggcgagg gtctcgccga cgcagacgat cggctccaga
     1441 ccggcccggt gggcggcccc ggtcttggcc gcgaccaggg nnnnnnnnnn nnnnnnnnnc
     1501 ccgccgttcg gaatggccga gaatgaccag gcccgcaccg gcgtcgacca gcatctcggc
     1561 ggcgatgtcg ccggtgaagg cgccggcggc ctcggcgcga cagtcctgtc cgccgacctc
     1621 gacccccgtg ccgcccagcg cctcgaccag ccggtggacc agggtcgccg gcggacagat
     1681 ggccacccgc gcgcccggcg gcctg
//
LOCUS       DAORNX010000570         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_50933_length_1695_cov_8.431098, whole genome shotgun sequence.
ACCESSION   DAORNX010000570 DAORNX010000000
VERSION     DAORNX010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50933_length_1695_cov_8.431098"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1448)
                     /gene="tig"
                     /locus_tag="PLO65_16785"
     CDS             complement(96..1448)
                     /gene="tig"
                     /locus_tag="PLO65_16785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRD29954.1"
                     /translation="MQIVEKSVEGLSRVYGVTVPAGELAARLDARITELAPQMNLKGF
                     RKGKVPPAHVRRVHGKALMQEVVQEALNETTQKVLDDNKLRPASQPDLKPESDMNAVL
                     SGGADLAYELAVELMPDFEPIDTSKLKLKRPVYTPTDKEVDEALAELAKQAGTFAPRT
                     GKSLKAKDGDQVLIDFVGSVGGVEFAGGKGEGFELTLGSNQFIPGFEAQLVGAKPGDD
                     VTVKVTFPEGYQSTELAGKDAEFAVKVHEVRAPVESEPDDALAQRLGLSDLATLRETV
                     TKNLEQGYANQSRFKLKRSLLDELDKGHDFPLPPRMVEAEFAGIWQQVEGDRTSGALP
                     PEDAGKSDEELRAEYRRIAERRVRLGLVLAEIGRKNNVQVTDQELTQAMQREAMQYGQ
                     QAQQVFDFFRSNPQAQAQLRAPLYEDKVVELIFGQATVTDKKVSKKEIEADDDLPEGY
                     "
BASE COUNT          230 a          577 c          578 g          310 t
ORIGIN      
        1 gcccggccag caggcccagc ccggtgatcg ccaggacgaa cgctctcgac atcacgcccc
       61 tcaactcccg accgtcgggt tcgcggcggc cggccctagt agccctcggg caggtcgtcg
      121 tcggcctcga tctccttctt ggagaccttc ttgtcggtca cggtcgcctg gccgaagatc
      181 agctcgacca ccttgtcctc gtacagcggc gcgcgcagct gggcctgagc ctgggggttc
      241 gagcggaaga aatcgaacac ctgctgggcc tgctggccat actgcatggc ctcgcgctgc
      301 atcgcctggg tcagctcctg gtcggtgacc tggacgttgt tcttgcggcc gatctcggcc
      361 agcaccaggc ccaggcgtac gcggcgctcg gcgatccggc ggtattcggc gcgcagttcc
      421 tcgtcgctct tgccggcgtc ttccggcggc agggcgccgg acgtccggtc gccctcgacc
      481 tgttgccaga tgccggcgaa ctcggcctcg accatgcgcg gcggcagcgg gaagtcgtgg
      541 cccttgtcca gctcgtcgag cagcgagcgc ttgagcttga agcgcgactg gttggcgtag
      601 ccctgctcga ggttcttggt caccgtctcg cgcagggtgg ccaggtccga caggcccagg
      661 cgctgggcga gagcgtcatc gggctcggat tcgaccggcg cgcgcacctc atggaccttc
      721 acggcgaact cggcgtcctt gccggccagt tcggtcgact ggtagccctc ggggaaggtc
      781 accttgacgg tgacgtcgtc gcccggcttg gcgccgacca gctgggcctc gaagccgggg
      841 atgaactggt tcgagccgag cgtcagctcg aagccctcgc ccttgccgcc ggcgaactcg
      901 acgccgccga cgctgccgac gaagtcgatc agcacctggt cgccgtcctt ggccttcagc
      961 gacttgccgg tgcggggggc gaaggtcccg gcctgtttcg ccagttcggc cagggcctcg
     1021 tccacttcct tgtcggtcgg ggtgtagacc gggcgcttca gcttcagctt gctggtgtcg
     1081 atcggctcga agtcgggcat cagctcgacc gccagctcat aggccaggtc cgcgccgccc
     1141 gagagcacgg cgttcatgtc cgattccggc ttcaggtccg gttgcgaggc cgggcgcagc
     1201 ttgttgtcgt cgaggacctt ctgggtggtc tcgttgagcg cctcctgcac cacctcctgc
     1261 atcagggcct tgccatgcac gcggcgcaca tgggccggcg gcaccttgcc cttgcggaag
     1321 cccttcaggt tcatctgcgg ggccagttcg gtgatccggg cgtcaagccg cgcggcgagc
     1381 tcgccggccg gaacagtgac gccgtagacg cggctcaggc cttcgacaga cttttcaacg
     1441 atttgcatcg acattctcaa gaataccgga cgagtggccg tggccgttgt catccgccag
     1501 gtgtggaaat gggtccgccg cccgggtcag gggcggcgca aagcgggggc ccttatgtca
     1561 tgcgggactt ttgcggccaa gcccccgttt ccggccgggc cggacgggcc gtcgcccgaa
     1621 gcccgcatgg cgaggcaggt ggtgcggatg agaggactcg aacctccacg ccttgcggcg
     1681 ctggaaccta aatcc
//
LOCUS       DAORNX010000571         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51005_length_1693_cov_5.227717, whole genome shotgun sequence.
ACCESSION   DAORNX010000571 DAORNX010000000
VERSION     DAORNX010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51005_length_1693_cov_5.227717"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLO65_16790"
     CDS             <1..785
                     /locus_tag="PLO65_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HRD29955.1"
                     /translation="GRDARGRKQYRYHPAWRATRDEAKFDRVAAFGRALPRLRRRVDA
                     DLRRRGLPREKVLASVVALLEATLIRVGNDEYARANRSFGLTTLRKRHVRVSTGGAVF
                     EFRGKSGKAHRTAFHDRRLARVIRACQDLRGQRLFKYVDEAGAVRAVQSGDVNDYIRA
                     AIGDGFTAKDFRTWFGTLAAAQALTREPRPDDAAAVKAATVRAIRVVAGLLGNTPAVC
                     RTAYVHPLVLEAFARGRPLPKASLQGRAFETALIRFLEAAGG"
     gene            817..1389
                     /locus_tag="PLO65_16795"
     CDS             817..1389
                     /locus_tag="PLO65_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRD29956.1"
                     /translation="MCNTFLSPNTPERVEWAFSQVRIPILWPETPRPNRGVTGDIRPT
                     DPACVVSPEGDGGRLDWLAWGFPRDKGGPVINFRSEGRVFPRQTRCLVMAAGFYETTD
                     PVQPGQKRKDWWLFTAPDAELFALAGCVRGDRFTVLTCEPGPDVQPYHGRQPVCLMPE
                     RWGDWLAGAPEADLIAPSPAGTFMVARKPT"
     gene            1444..>1693
                     /locus_tag="PLO65_16800"
     CDS             1444..>1693
                     /locus_tag="PLO65_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29957.1"
                     /translation="MDGSPSINERLHAANEVRGWPVASALRAGMHLRARCLNPRCERV
                     TVVDASWWLAGNGADERLHMLGRRMRCSACGAREAAIEV"
BASE COUNT          234 a          602 c          609 g          248 t
ORIGIN      
        1 tcggtcgcga cgcccgaggc cgcaagcagt atcgctatca ccccgcctgg cgggcgaccc
       61 gcgacgaggc caagttcgac cgggtggccg ccttcggccg ggccctgccc cgcctgcgcc
      121 gccgtgtcga cgccgacctg cgacggcgcg gcctgccgcg ggagaaggtg ctggcctcgg
      181 tggtcgccct gcttgaggcg accttgatcc gtgtcggcaa cgacgaatac gcgcgggcca
      241 atcgcagttt cggtttgacc acgctgcgaa agcggcatgt ccgcgtctcg acgggcggcg
      301 ccgtgttcga attccgcggc aagagcggca aggcgcaccg cacggcgttt cacgaccgcc
      361 ggctggcccg ggtgatccgg gcctgtcagg atctgcgcgg ccagcggctg ttcaaatatg
      421 tcgacgaggc cggcgcggtg cgcgcggtcc agtccggcga cgtcaacgac tacatccggg
      481 cggccatcgg cgacggcttc acggccaagg acttccgcac ctggttcggc accctggccg
      541 ccgcccaggc cctgacccgg gagccgcgcc ccgatgacgc cgcggccgtg aaggcggcga
      601 cggtccgggc catcagggtc gtcgcgggtc tgctgggcaa tactccggcg gtctgccgga
      661 cggcctatgt gcatccgctg gtgctggagg ccttcgcccg ggggcggccg ctgccgaagg
      721 cgtcgttgca ggggcgcgcc ttcgagacgg cgctgatccg gtttctcgaa gcggccggcg
      781 gctgacgcct cccgatcgaa agtctaggct ggcgtcatgt gcaacacctt cctcagcccc
      841 aacacgcccg agcgcgtcga atgggcgttc tcgcaggtgc ggattccgat cctctggccg
      901 gagacgccgc gaccaaaccg gggcgtcacc ggcgacatcc gccccaccga tccggcctgt
      961 gtcgtgtctc ccgagggcga cggcgggcgg ctggactggc tggcctgggg cttcccgcgc
     1021 gacaagggcg ggccggtgat caacttccgc tccgagggcc gcgtgtttcc gcgccagacc
     1081 cgctgcctgg tcatggccgc cggcttctac gagacaaccg acccggtgca gcctggccag
     1141 aagcgcaagg actggtggct gttcaccgcg cccgacgccg aactgttcgc gctggccggc
     1201 tgcgtgcgcg gcgaccggtt cacggtgctg acctgcgagc cgggccccga cgtccagccc
     1261 taccacggcc gccagccggt ctgcctgatg ccggaaagat ggggcgactg gctggccgga
     1321 gcgccggaag cggacctgat cgctcccagc cccgccggaa ccttcatggt cgcccggaag
     1381 ccgacctgag cccgattgac tcccgctcca cggcgagaac aaaatgggaa cacgaggttt
     1441 cccatggatg gctccccatc gatcaacgaa cggctgcatg cggccaacga agtgcgcgga
     1501 tggccggtcg cctcggcgct gcgggccggc atgcacctgc gcgcgcgttg cctgaatccg
     1561 cgctgcgagc gggtcacggt ggtcgacgcg tcctggtggc tggccggcaa cggcgcggac
     1621 gagcggctgc acatgctggg ccggcgcatg cgctgctcgg cctgcggggc gcgggaggcg
     1681 gccatcgagg tct
//
LOCUS       DAORNX010000572         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51181_length_1688_cov_5.431108, whole genome shotgun sequence.
ACCESSION   DAORNX010000572 DAORNX010000000
VERSION     DAORNX010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51181_length_1688_cov_5.431108"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PLO65_16805"
     CDS             <1..>1020
                     /locus_tag="PLO65_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29958.1"
                     /translation="HRRADVRAINDAIRTELQDRGELAQGNVPAVGSDAGALTFQTND
                     GKREFAPGDRIVFLENNRDLGVKNGMLGTVEHVEAGRIVAQLDGRGGDSVSVPMGDYQ
                     AIDHGYATTIHKNQGATVDRAYVMASGTMDRHLTYVAMTRHRDGVQLYAAQDEFTNAG
                     RLVEHGAAPFEHDPQKSGSYFVTLENDKGEQRTLWGVDLERAMKEAAPEIGEKIGLQH
                     EGSTPVTLPDGTQTHRNTWKVQDAGELAYSQLERRLSRSGVKETTLDYTRDFAERRGI
                     AEQMGVRSEIEIPAERAGLRAERESTAGDHALDRRSSQKVRADLAQDLRADPREDLAG
                     DRQQR"
     assembly_gap    1021..1074
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1075..>1688
                     /locus_tag="PLO65_16810"
     CDS             <1075..>1688
                     /locus_tag="PLO65_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29959.1"
                     /translation="RAGEDGPQIDQKGPQQAEKQRRGMFAGLKLNARPAPSQERGEAS
                     RMQRRPEREGSLRQAPAPDRLAERARGQSPLEAAVDRYSRAYQSIDQHRREGLPVLDM
                     QRQEIGDAGRQLDQVQGGMKDLLRSTLQNDPATVRAMTELSGRERVAQVIDGMKRENA
                     ALQDPNVRAERFVERWQELQGQRRELRGWQHDDARAKVESQMNG"
BASE COUNT          322 a          505 c          587 g          220 t
ORIGIN      
        1 gcatcgccgg gccgatgttc gggcgatcaa tgacgcgatc aggaccgagc tacaggaccg
       61 gggcgagctg gcgcagggca acgtgcccgc cgtgggctcg gacgccggcg cgctgacctt
      121 ccagaccaat gacggcaagc gggagttcgc gccgggcgac cgcatcgtgt tccttgagaa
      181 caatcgcgac cttggcgtga aaaacgggat gcttggcacc gtcgagcatg tcgaggccgg
      241 ccggatcgtc gcgcagctcg acggccgggg cggcgatagc gtcagcgtgc cgatgggcga
      301 ttatcaggcg atcgaccacg gttatgcgac gacgattcac aaaaatcagg gcgcaaccgt
      361 ggaccgcgcc tatgtgatgg cgtcggggac gatggaccgg catctaacct atgtcgccat
      421 gacccggcat cgtgacgggg tgcagctcta cgcggcacag gacgagttca ccaatgccgg
      481 ccggctggtc gagcatgggg ccgcgccctt cgagcatgac ccgcagaagt ccggcagcta
      541 tttcgtgacg ctggaaaacg acaagggcga gcagcgcacc ctatggggcg tcgatcttga
      601 gcgcgccatg aaggaggccg cgccggagat cggggagaag atcggcctac agcatgaggg
      661 ttccaccccc gtcaccttgc cggatgggac gcagacgcac cgcaacacat ggaaggttca
      721 ggacgccggc gagctggcct atagccagct agaacgccgc ctgtcccggt cgggcgtgaa
      781 ggaaacgacg ctcgattata cccgcgactt tgccgagcgg cgcgggatcg cggagcaaat
      841 gggcgtccgc agcgagatcg agattccggc cgagcgcgcc gggctacgtg cggaacggga
      901 gtcgacggcc ggggatcatg cccttgatcg gagatcctcg caaaaagttc gcgcggatct
      961 cgcgcaggat cttcgtgcag atccgcgcga ggatctcgcc ggcgatcgac agcagcgcca
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccgcg
     1081 ccggcgagga cggcccacag atcgaccaga aaggcccgca gcaggccgag aagcagcggc
     1141 gcggtatgtt cgccggcctc aagctcaatg cacgccctgc gccctcacag gagcgcggcg
     1201 aggcatcgcg gatgcagcga cggccggaaa gggaagggag cttgcggcag gcgcctgcgc
     1261 ccgaccggct ggccgagcgg gcgcgggggc agtcgccgct tgaggccgcc gttgaccgct
     1321 attcgcgggc ctatcagtcg atcgaccagc accggcgcga gggcttgccg gtcctcgaca
     1381 tgcagcggca ggaaataggc gatgccggcc ggcagctcga ccaggtgcag ggcggcatga
     1441 aagatttgct gcgctcgacg ttgcaaaacg acccggcgac ggtgcgcgcc atgaccgagc
     1501 tttccggccg ggagcgtgtc gcgcaggtta tcgacgggat gaagcgcgaa aacgccgcgc
     1561 tgcaagaccc gaatgtcagg gccgagcggt ttgttgaacg ctggcaggaa ctacagggcc
     1621 agcgccggga gcttcgcggc tggcagcacg acgatgcccg cgccaaggtc gaaagccaga
     1681 tgaacggc
//
LOCUS       DAORNX010000573         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51247_length_1686_cov_5.879215, whole genome shotgun sequence.
ACCESSION   DAORNX010000573 DAORNX010000000
VERSION     DAORNX010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51247_length_1686_cov_5.879215"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLO65_16815"
     CDS             complement(<1..222)
                     /locus_tag="PLO65_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRD29960.1"
                     /translation="MRAATILERPWRPPEAVLSAFADDPWTCAFLSGGAGERARWSYV
                     LRQPDQTLTLGPDDAGDPFRTMKALLGETR"
     gene            complement(260..>1686)
                     /locus_tag="PLO65_16820"
     CDS             complement(260..>1686)
                     /locus_tag="PLO65_16820"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29961.1"
                     /translation="GGWSWSIFQGGRDPYVILITIYIFAPYFTTAVVGDPVRGQAMIA
                     TVSTLNSIFIALTAPILGAAIDRYGARKPMMAATVLGMAPMIWALWFARPGGEGLPVL
                     AGLWLFGLIGVLFAFCEVIHNSLLISAAGHRHAPQASGLALSAGNFFSVITLTFCLMA
                     FALPGNPAVAGWPLIPDHPLFGLDPSQAETSRVVAPIAASLLVLLSIPLFLFTPDAPK
                     TGISLIAALKQSAAHLGGVAWSLISAAARGPARLREVLGVLVTERDAVIYLGARMLFN
                     DGMTALLIFSGIYTAGVMQWQVLEMLAYGILLSVFAVAGGLCGAVFDNLFGPRNAVRF
                     EIAGSLVCLVAWLGMRRDRILYFWPYDADAHAPLWNGPMFRTLPELIYLAIGFGVAVF
                     VTAQYASSRTLLTRLAPPEKLPAFFGLYALSGTLTVWIGSMLVGLFTSLTHSQSGGFI
                     PIAGLLALGLAGMMFVRGGGKPAV"
BASE COUNT          304 a          585 c          604 g          193 t
ORIGIN      
        1 ccgggtctcg cccagcagcg ccttcatcgt ccgaaacggg tcgccggcgt cgtccggtcc
       61 gagggtcagg gtctggtcgg gctggcgcag gacataggac cagcgcgcgc gctcgcccgc
      121 gccgcccgaa aggaaggcgc aggtccacgg atcgtcggcg aaggccgaca gcaccgcttc
      181 gggaggccgc cagggccgct ccaggatcgt cgccgcgcgc atgggcggca gatagccccc
      241 cggcggcccg ccgcaaaggt cagaccgccg gtttgccgcc gccccgcacg aacatcatgc
      301 cggccaggcc gagcgccagc aggccggcga tcgggatgaa gccccccgac tggctgtggg
      361 tcaggctggt gaacaggccc accagcatcg aaccgatcca caccgtcagg gttcccgaca
      421 gggcgtacag gccgaagaag gccggcagct tctcgggcgg cgccagccgc gtcagcaggg
      481 tgcggctcga ggcgtactgg gcggtgacga acaccgccac gccgaagccg atggccagat
      541 agatgagctc cggcagggtg cggaacatcg gcccgttcca cagtggcgcg tgggcgtcgg
      601 cgtcgtaggg ccagaagtac aggatccggt cccgccgcat gcccagccag gccaccaggc
      661 agaccagcga tccggcgatc tcgaaccgca ccgcgttgcg cggcccgaac aggttgtcga
      721 acaccgcgcc gcacaggccg ccggcgaccg cgaacacgct gagcaggatg ccgtaggcca
      781 gcatctcgag gacctgccac tgcatcaccc ccgcggtgta gatgcccgag aagatcagca
      841 gggcggtcat gccgtcgttg aacagcatgc gcgcgcccag gtagatcacc gcgtcgcgct
      901 cggtgaccag cacgccgagc acctcgcgca gccgggccgg cccgcgcgcc gccgccgaga
      961 tcagcgacca ggcgacgccg cccagatgcg ccgccgactg cttgagcgcc gcgatcaggc
     1021 tgatgccggt cttcggcgcg tcgggggtga acaggaacag cggtatcgac agcagcacca
     1081 gcaggctggc ggcgatcggc gcgacgaccc gcgaggtctc ggcctggctc ggatcgaggc
     1141 cgaacagcgg gtggtcggga atcagcggcc agccggccac cgccgggttg ccgggcaggg
     1201 cgaaggccat caggcagaag gtcagggtga tgaccgagaa gaagttgccc gccgacaggg
     1261 ccaggcccga ggcctggggc gcgtgccggt gtccggcggc gctgatcagc agcgaattgt
     1321 ggatcacctc gcagaaggcg aacagcacgc cgatcaggcc gaacagccat agtccggcca
     1381 gcaccggcag cccctcgccg cccggcctgg cgaaccacag ggcccagatc atcggggcca
     1441 tgcccagcac cgtcgccgcc atcatcggct tgcgcgcgcc gtaacggtcg atcgcggcgc
     1501 cgaggatcgg ggcggtcagg gcgatgaaga tcgaattgag cgtgctgacc gtggcgatca
     1561 tcgcctggcc cctgaccgga tcgccgacca cggcggtcgt gaaatagggg gcgaagatgt
     1621 agatggtgat caggatcaca taggggtcgc gcccgccctg gaagatcgac cagctccagc
     1681 cgccga
//
LOCUS       DAORNX010000574         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51368_length_1683_cov_4.187346, whole genome shotgun sequence.
ACCESSION   DAORNX010000574 DAORNX010000000
VERSION     DAORNX010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51368_length_1683_cov_4.187346"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLO65_16825"
     CDS             complement(<1..555)
                     /locus_tag="PLO65_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD29962.1"
                     /translation="MSEFDFDALVVGAGAVGLATGYALAGRGLSVVVLEKEKIIGQGV
                     SSRNSEVIHAGLYYPTGSLKARLCVQGRRRLYAFLDRHHVDYRRCGKLVVANGPDEIE
                     RLDAILEQARINDVEGMERLTGAAAMAMEPGLRCDMALLSPQSGVFDSHGYMTALVGE
                     IEAHGGAVVTSTPFEGATPLPGGGF"
     gene            622..>1683
                     /locus_tag="PLO65_16830"
     CDS             622..>1683
                     /locus_tag="PLO65_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD29963.1"
                     /translation="MAFDIATGQRPTGASAAALELYEMVLAGHQCFTGDPVRNLRATL
                     ADSPDFVMAHVFLGYLHAAGTDAFTLPVALDCHAKALALPASDREAGHVTALGHMARG
                     EIAAAAKVLAGVSAAYPRDALALQIGQLLDFLQGDARMLRDRIGIALPHWDETMPGYH
                     AVLGLHAFGLEETGHYAAAETAGRLAIGMEPRNGWARHAVAHVMEMQDRREDGVAWMR
                     GDLESWTGDSYFQVHNWWHLALFHLGLGDMEAVLALYDEPIWGDRSTLAYNMVDASAL
                     LWRLHLMDVDVGDRWRDLAERWEPAVAESLYAFNDAHAAMAFVGAGRTDALEALREAQ
                     RRALARPGDNAAFQQVGEPV"
BASE COUNT          245 a          598 c          603 g          237 t
ORIGIN      
        1 aaagccgccg cccggcaggg gcgtggcgcc ctcgaacggc gtggaggtga ccaccgcccc
       61 gccatgggct tcgatctcgc cgacaagggc ggtcatgtag ccgtggctgt cgaacacccc
      121 gctctggggc gacagcaggg ccatgtcgca cctgaggccc ggctccatgg ccatggccgc
      181 cgcgcccgtc agcctctcca tgccctcgac atcgttgatc cgggcctgtt cgaggatggc
      241 gtccagccgt tcgatctcgt cggggccgtt ggccaccacc agcttgccgc agcggcggta
      301 gtcgacatgg tgcctgtcga ggaaggcgta gagccgccgc cggccctgca cgcagagccg
      361 cgccttgagc gagccggtcg gatagtagag cccggcgtgg atgacctcgg aattgcgcga
      421 ggacacgccc tggccgatga tcttctcctt ctccagcacc accacggaca ggccgcgacc
      481 ggccagggcg tagcccgtgg ccaggcccac cgcgccggcg ccgaccacca gggcgtcgaa
      541 gtcgaactcg ctcatgtcca aggcgtagac cagcgcgcac cgtcaggcca agctgtgatt
      601 tcccgacaca gcgagcgacc catggccttc gacatcgcca ccggacagcg gcccacgggc
      661 gcgagcgccg cggccctcga gctctacgag atggtgctgg ccgggcacca gtgcttcacc
      721 ggagacccgg tgcgcaatct gcgcgcgacg ctggccgaca gcccggactt cgtcatggcc
      781 catgtgttcc tcggctatct gcacgcggcg ggcaccgacg ccttcaccct gccggtggcg
      841 ctggactgcc acgcgaaggc cctggcgctg ccggccagcg accgcgaggc cggccatgtg
      901 acggcgctgg gccatatggc gcgcggcgag atcgcggcgg cggcgaaggt gctggccggg
      961 gtctccgcgg cctacccgcg cgacgccctg gcgctgcaga tcggccagct gctcgacttc
     1021 ctgcagggcg acgcgcggat gctgcgcgac cggatcggga tcgccctgcc ccattgggat
     1081 gagaccatgc ccggctatca cgcggtgctc gggctgcacg ccttcgggct ggaggagacc
     1141 ggccactacg ccgccgccga aacggccggg cgtctcgcga tcgggatgga gccgcgcaac
     1201 ggctgggccc ggcacgcggt ggcccatgtg atggagatgc aggaccggcg cgaggacggc
     1261 gtggcctgga tgcgcggcga tctggagagc tggaccggcg acagctattt ccaggtgcac
     1321 aactggtggc atctggcgct gttccacctc ggcctgggcg acatggaggc ggtgctcgcc
     1381 ctgtacgacg agccgatctg gggcgaccgc tcgacgctgg cctacaacat ggtcgacgcc
     1441 tcggccctgc tgtggcggct gcacctgatg gacgtcgatg tcggcgaccg ctggcgtgac
     1501 ctggccgaac gatgggagcc ggccgtggcc gagagcctct acgcgttcaa cgacgcccat
     1561 gcggcgatgg ccttcgtcgg cgccgggcgg accgatgcgc tggaggcctt gcgcgaggcc
     1621 cagcggcgcg cgctcgcgcg gccaggcgac aacgccgcct tccagcaggt cggcgagccc
     1681 gtg
//
LOCUS       DAORNX010000575         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51392_length_1682_cov_8.401352, whole genome shotgun sequence.
ACCESSION   DAORNX010000575 DAORNX010000000
VERSION     DAORNX010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51392_length_1682_cov_8.401352"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PLO65_16835"
     CDS             <1..893
                     /locus_tag="PLO65_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD29964.1"
                     /translation="ETARTPDELARAMALGRVQFTITIPADFSRRVVRGDRAQILVEA
                     DAADPSATGSAIAALAALPQEALSHDLTGALAPRAGGTPPFEVVVHRRYNPEAIAAYN
                     IVPGLLGVILSMTLVMMTALGVTREYERGTMEGLLATPVKPIEVMIGKLAPYVLVGAI
                     QTVLILAMARFLFDVPFAGGWDALTVGILLFIVGSLALGFLISTVARNQLQAMQMSVF
                     YIFPSLLLSGFMFPFRGMPDWAQALGSAIPVTHFLRVVRGALLKGLGFAELWQSLAAL
                     GLFVLIVVSLAMARYRTTLD"
     gene            1033..>1682
                     /locus_tag="PLO65_16840"
     CDS             1033..>1682
                     /locus_tag="PLO65_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD29965.1"
                     /translation="MKFKLLAGAALAVTTLAATGAAAQDSGWYGAVDVGYHWSDDTTA
                     DTGFGPLSIDPDDDWIGLARLGYRLTPNWRLEVEGGWRPGELQSPPFDATGDAEVISL
                     MGNVIYDFMPDSVMHPFIGVGAGAARGQLGVLLVPFVAVDDEDVTWAWQGIAGLTAKA
                     SDRMNVDLTYRYFQTGDFDFENPGIAGNLSGSYNDQSVTVGVRYSFQEEAAPPPPPP"
BASE COUNT          240 a          578 c          574 g          290 t
ORIGIN      
        1 ccgagacggc gcgcacgccg gacgagctcg cgcgggccat ggccttgggc cgggtgcagt
       61 tcaccatcac catcccggcc gacttcagcc ggcgcgtggt gcgcggagac cgcgcccaga
      121 tcctcgtcga ggccgacgcc gccgacccgt cggccacggg ctcggcgatc gccgcgctcg
      181 ccgccctgcc ccaggaagcg ctcagtcacg acctgaccgg cgcgctcgcc ccgcgggccg
      241 gcgggacgcc gccgttcgag gtggtcgtgc accggcgcta caatcccgag gccatcgccg
      301 cctacaacat cgtgccgggg ctgctggggg tgatcctgtc gatgaccctg gtgatgatga
      361 cggccctcgg ggtgacccgg gaatacgagc ggggcaccat ggaaggcctg ctggccaccc
      421 cggtgaagcc gatcgaggtg atgatcggca agctcgcgcc ctatgtgctg gtcggggcga
      481 tccagacggt gctgatcctg gccatggccc ggtttctgtt cgacgtgccg ttcgccggcg
      541 gatgggacgc gctgacggtc ggcatcctgc tgttcatcgt cggcagcctg gcgctcggct
      601 tcctgatctc gaccgtcgcg cgcaaccagc tgcaggccat gcagatgtcg gtgttctata
      661 tctttccgtc gctgctgctg tcgggcttca tgttcccgtt ccgcggcatg cccgactggg
      721 cccaggccct gggcagcgcc atccccgtga cccacttcct gcgggtcgtc cgcggggcgc
      781 tgctcaaggg gctcggattc gccgaactgt ggcagagcct cgcggctctt ggcctgttcg
      841 tcctgatcgt ggtttccctg gcgatggcgc gctaccgcac aaccctcgac tgagaaactc
      901 gccattggcg attatgccag agaaaatagg cgttggtgtg gcttcgctga cacttggcgg
      961 tgggctgatg gttgatataa cacctattcg agcggcctgt ggccgcatcg cgggcaggag
     1021 agggctctca ctatgaaatt caagcttctg gcgggtgcgg cgctggccgt cacgacgctc
     1081 gcggccacgg gcgccgcggc tcaggacagc ggctggtacg gcgcggtcga cgtcggctac
     1141 cactggtccg acgacacgac ggcggacacc ggctttggtc cgctctcgat cgatcccgat
     1201 gacgactgga tcggcctggc ccgcctcggc taccggctga ccccgaactg gcgcctcgaa
     1261 gtcgagggcg gctggcgtcc gggcgagctg cagtcgccgc cgttcgacgc gaccggcgac
     1321 gccgaagtca tctccctgat gggcaatgtc atctacgact tcatgcccga cagcgtcatg
     1381 catccgttca tcggcgtggg cgccggtgcg gcgcgcggcc agctgggcgt gctgctggtg
     1441 ccgttcgtgg ccgtcgacga cgaggacgtg acctgggcgt ggcaaggcat cgccggcctg
     1501 accgccaagg ccagcgaccg gatgaacgtc gatctgacct accgctactt ccagaccggc
     1561 gatttcgact tcgagaaccc cggcatcgcg ggcaacctgt cgggcagcta caacgaccag
     1621 tcggtgacgg tcggcgtgcg ctacagcttc caggaagaag cggccccgcc gcccccgccg
     1681 cc
//
LOCUS       DAORNX010000576         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51566_length_1677_cov_5.440814, whole genome shotgun sequence.
ACCESSION   DAORNX010000576 DAORNX010000000
VERSION     DAORNX010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51566_length_1677_cov_5.440814"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..128
                     /locus_tag="PLO65_16845"
                     /pseudo
     CDS             1..128
                     /locus_tag="PLO65_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            683..>1677
                     /locus_tag="PLO65_16850"
     CDS             683..>1677
                     /locus_tag="PLO65_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD29966.1"
                     /translation="MTDPAASDAPPPPPPVQAVPRIAYLAVASALFMEFIDSTALSTA
                     LPTLARAFHADPVHLKLALTSYILALAVFVPISGWAAEKFQPRRVFLAAMVVFLAGSA
                     LCGFSSSLGELVGARIVQGAGGALMTPVARLIVVGSAPRNDLIRAMGWFTMPALAGPL
                     IGPPLAGFVLSVADWPWIFFINIPVGLLGMLAVLRFVPRLPGLDPGAFDWSGFALSAA
                     SITGIVVVAETGGLNLVPWWAQIGLVAASLACGWLYLRLARRKVRPILNLALLRYPTF
                     RASLLGGTLMRLGVGAGPFLMPLLLQVALGWTPLQAGSVTLAGGLGVFVSRPFAAP"
BASE COUNT          181 a          621 c          585 g          290 t
ORIGIN      
        1 cgcaagcgct cgagacgccg ggggggctga agatccagac tatccatgcc ttctgcgaag
       61 cggtgctgaa gcgctttccg ctcgaggcca atatcgcggg gcatttcgac atgctcgacg
      121 acagcgccgc cgaggtgctg atcgctgaag tccgccgcgc cctgttgtcc ggtcgcgacg
      181 aaggcggccg gtcggccctt gccggcgcgc tcgcccacgt cctcgaccgg gtcggggaat
      241 tcggcctcga tgcgcttctc tccgccatcg tggccctgcg cgatccgctg cgcggctggc
      301 ggccggccgg ctggtcggtc gagcagctcg ccgagttcgg caaggcccag ctgggcgtcg
      361 gctggcgcaa ggggtcgcgg caggtggccg tcagcgcggc ccggcgcgag atcagcgcct
      421 acggggtctc ccgcgaggac acgatcttcg gcctgacctt caccgtcagc ggtcgcgccc
      481 cggccaagac gcgctacgag cagcggctgc caaaggccta ctaggccgat cgacggtcaa
      541 tgccggacgg cgcggccccg ccagccggtc ccgcgtgggc gaggacccgg gccgcccggc
      601 cggctcgaac cacggccttc ggacaggaat ccgtttgatg ggcggggcct gacggggcta
      661 ccaccgtgcc gtgccagatc ccgtgaccga cccggccgcg tccgatgcgc cgcctcctcc
      721 gccgcctgtc caggccgtgc cgcgcatcgc ctatctggcg gtcgccagcg ccctgttcat
      781 ggagttcatc gactccaccg ccctgtcgac ggccctgccg accctggcgc gggcattcca
      841 cgccgatccc gtccatctca agctggccct gacgtcctat atcctggccc tggccgtgtt
      901 cgtgccgatc agcggctggg ccgccgagaa gttccagccc cgtcgggtat tcctcgcggc
      961 gatggtcgtc tttcttgccg ggtcggccct gtgcggcttt tcctccagcc tgggcgaact
     1021 ggtcggcgcg cggatcgtgc agggcgccgg cggcgcgctg atgacgccgg tggcccgcct
     1081 gatcgtcgtc ggctcggctc cccgcaacga cctgatccgg gccatgggct ggttcaccat
     1141 gccggccctc gcgggcccgc tgatcggccc gccgctggcc ggcttcgtcc tctcggtggc
     1201 cgactggccg tggatcttct tcatcaacat tccggtcggc ctgctgggca tgctcgccgt
     1261 cctgcggttc gtgccgcgcc tgccgggcct cgacccgggc gcattcgact ggagcggctt
     1321 cgccctgtcc gcggccagca tcaccggaat cgtcgtggtg gccgagaccg gcggcctgaa
     1381 cctggtcccc tggtgggcgc agatcggcct ggtcgcggcc tcgctggcct gcggctggct
     1441 ctatctgcgg ctggcccggc gcaaggtccg tccgatcctc aacctcgcgc tgctgcgcta
     1501 tccgacgttt cgcgccagcc tgctgggtgg aaccctgatg cggctgggcg tcggcgcggg
     1561 accgttcctg atgccgctgc tgctgcaggt ggcgctgggc tggacgccgc tgcaggccgg
     1621 atcggtgacc ctcgcgggcg gcctgggcgt gttcgtctcc cgaccgttcg cggcgcc
//
LOCUS       DAORNX010000577         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51593_length_1676_cov_3.975941, whole genome shotgun sequence.
ACCESSION   DAORNX010000577 DAORNX010000000
VERSION     DAORNX010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51593_length_1676_cov_3.975941"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLO65_16855"
     CDS             <1..385
                     /locus_tag="PLO65_16855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRD29967.1"
                     /translation="FDAVLLDAPCSATGTFRRHPDVLWAAAPGDVASLAAVQTRMLDS
                     AAARVKPGGRLVYCVCSLEPEEGEAQVRGFLARHPDFTTVPVAPGEGGAPAASVGPEG
                     WLRILPHHLAGGIDGFFAARMVRRG"
     gene            427..1086
                     /gene="rpe"
                     /locus_tag="PLO65_16860"
     CDS             427..1086
                     /gene="rpe"
                     /locus_tag="PLO65_16860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRD29968.1"
                     /translation="MAAPIIAPSILASDFSRLGEECRAVEAAGADWLHVDVMDGHFVP
                     NITLGPDVVKALRPHVSIPFDVHLMISPADPYLEAFAAAGANILSIHPEAGPHLNRSL
                     KRIRELGCKAGVVFNPSTPVEAVEWILEDVDLLLVMTVNPGFGGQSFIPAQLKKIERL
                     RRMIDASGCAIALEVDGGVTPVTAPQCVAAGATALVAGSAVFKGGPDAYAGNIRALKG
                     V"
     assembly_gap    1273..1400
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1401..>1676
                     /locus_tag="PLO65_16865"
     CDS             <1401..>1676
                     /locus_tag="PLO65_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HRD29969.1"
                     /translation="RMGWMGDLLAAGDAGARLGLRLAMDWRRVFGRWNSFSWGPDVIE
                     RRVFNLACAARRLTAGASDAERTLLLDDLARQARHLASIGQAPERALE"
BASE COUNT          186 a          589 c          540 g          233 t
ORIGIN      
        1 cttcgacgcc gtactgctcg acgcgccctg ttcggccacc ggcaccttcc gtcgccaccc
       61 cgacgtgctg tgggccgcgg ccccgggcga cgtcgccagc ctggccgcgg tccagacccg
      121 gatgctcgat tccgccgccg cccgcgtgaa gccgggcggg cggctggtct actgcgtctg
      181 ttcgctggag ccggaggagg gcgaggccca ggtgcgtggc ttcctcgcgc gccacccgga
      241 cttcaccacc gtcccggtcg cgcccggcga aggcggggct cccgccgcca gcgtcggccc
      301 ggagggctgg ctgcgcatcc tgccccatca cctcgccggc gggatcgatg gcttcttcgc
      361 cgcccggatg gtccgcaggg gctgagccgt ccttgttccc cggggccggt ccggctagac
      421 caaggcatgg ccgccccgat catcgccccg tccatcctgg cgtctgactt ttcccgtctt
      481 ggcgaggagt gccgcgccgt agaggccgcc ggcgccgact ggcttcacgt cgacgtgatg
      541 gacggccact tcgtgcccaa catcacgctc ggccccgatg tggtgaaggc cctgcgcccg
      601 cacgtctcaa tcccgttcga cgtgcatctg atgatcagcc cggccgaccc ctatctggag
      661 gcttttgccg ccgcgggggc caatatcctc agcatccatc cggaggccgg accgcacctg
      721 aaccgctcgc tgaagcgcat ccgcgagctc ggctgcaagg ccggcgtggt gttcaacccc
      781 tcgacccccg tcgaggccgt cgaatggatc ctcgaggatg tcgacctgct gctggtgatg
      841 accgtcaacc cgggtttcgg cggccagagc ttcatccccg cccagctgaa aaagatcgag
      901 cggctgcgcc ggatgatcga cgcctcgggc tgcgccatcg cgctcgaggt cgacggcggg
      961 gtcacgccgg tcaccgcgcc gcagtgcgtc gccgcagggg ccacggccct ggtcgccggc
     1021 tcggccgtgt tcaagggcgg ccccgacgcc tacgccggca acatccgcgc cctgaagggc
     1081 gtctgagccc ggcgggatgg acgccccccg actgccttcc gcctcgtccg ccgccctcgc
     1141 cgtccaggcc gccggcgaag gcgttcgccg cctggccgag cgggaatggt tcggcacgcc
     1201 cctgcaccgg ctgatgctca accggccccg gcccgacggc atggcggcca acccgcgcga
     1261 gtacacctgg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn cgcatgggct ggatgggcga cctgctggcc gcgggcgatg
     1441 cgggagcccg gctgggcctg cggctggcga tggactggcg acgggtgttc ggccgctgga
     1501 acagcttctc ctggggcccg gacgtgatcg agcggcgggt gttcaacctg gcctgcgccg
     1561 cgcgccggct gaccgccggg gcgtccgacg ccgagcggac cctgctgctc gacgatctgg
     1621 cgcggcaggc gcggcatctc gcctccatcg gccaggcccc cgagcgggcg ctggag
//
LOCUS       DAORNX010000578         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51653_length_1674_cov_7.137739, whole genome shotgun sequence.
ACCESSION   DAORNX010000578 DAORNX010000000
VERSION     DAORNX010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51653_length_1674_cov_7.137739"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLO65_16870"
     CDS             <1..242
                     /locus_tag="PLO65_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010792166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HRD29970.1"
                     /translation="QGVSLKARLLFVSWIKVDGKLVYKAEPKPTETDERDPEVPVTSD
                     APAAQQASAPEPSEPVADAADDAADAPALAVAESF"
     gene            298..909
                     /locus_tag="PLO65_16875"
     CDS             298..909
                     /locus_tag="PLO65_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HRD29971.1"
                     /translation="MITIPGQLAIKTIHGRNGDFNVGRLATSIGEFVVKNAELDQYRE
                     GKYDGDFVIVEIRPSTYNANGRMVIEIRAHLGGMTLSNIDALSRDEARRLSPQEVDPI
                     DEEAQAPVPATPPAKPKAKPRSPRDPLVDTTPFGSEPAPVSAAASAEADDAALFGALW
                     PLGETVKLDATVDRRVLRQQRDRLDKLGYEFAPLSQDWHLKAA"
     gene            999..1364
                     /locus_tag="PLO65_16880"
     CDS             999..1364
                     /locus_tag="PLO65_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29972.1"
                     /translation="MGWTFVRQTRDQLIRELLAPQASERACCEVIDHTLDGDVLWTVV
                     RVTARQAGVMGLVAGESVCYIGCHLLESSGGDWGYKSLDESVHPYYYSCPLRYLDMAP
                     VQSPEWRERVHRFHAGRAV"
     gene            1449..>1674
                     /locus_tag="PLO65_16885"
     CDS             1449..>1674
                     /locus_tag="PLO65_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29973.1"
                     /translation="MPAPSSAKPLYRIDECPDLMADGCVGDEQGNLVFLSIWARDTAV
                     QEFLARLTLGRDEQGLDQFHVITEQGASIPV"
BASE COUNT          278 a          590 c          513 g          293 t
ORIGIN      
        1 agcagggtgt gagcctcaag gcccgcctgc tgttcgtcag ttggatcaag gtcgacggca
       61 agctcgtcta caaggccgaa cccaagccga ccgagaccga cgagcgcgac ccggaagtcc
      121 ctgtgacgtc cgacgcgccc gctgcgcagc aagcctcggc accggagcct tccgagcccg
      181 tcgccgatgc tgccgacgac gcagccgatg cccccgcatt ggccgttgcc gagtcgttct
      241 gatccgcaag gccccatccc cggggccttg tcttctcttc gtcagcagga gaccttcatg
      301 atcaccatcc ccggccaact ggccatcaag accatccacg gcaggaacgg cgacttcaac
      361 gtcggccgcc tggcgacctc gatcggcgag ttcgtcgtga agaacgccga actcgatcag
      421 taccgcgagg gcaagtacga cggtgatttc gtcatcgtcg agattcgccc ctccacgtac
      481 aacgccaacg gccgcatggt catcgagatc cgcgcccatc tgggcgggat gacgctgtcc
      541 aacatcgacg ccctgagccg cgacgaagcc cgccggctga gtccgcagga agtcgatccg
      601 atcgacgagg aagcgcaggc gcccgtgccg gcaacgcccc cggccaagcc gaaggcgaag
      661 ccgcgcagtc cgcgcgatcc cctggtcgat accacgccgt tcggcagcga accggctccc
      721 gtgtccgctg cggcctcggc cgaggcagac gacgcggcgc tgttcggtgc cttgtggccc
      781 ctgggcgaga ccgtgaagct cgatgcgacc gtggatcgtc gtgtgctgcg tcagcagcgc
      841 gaccgtctcg acaagctggg ctacgagttc gctccgttgt cccaggactg gcacctcaaa
      901 gctgcctgat ccatccgccg ccttgcgcgg cattccgcca cccgccgggg ttctcccccg
      961 acggggaggg ctccggccat ttcttcaagg agaccttcat gggctggacc ttcgttcgtc
     1021 agacgcgcga tcagttgatc cgcgagctgc tcgctccgca ggcgtccgaa cgcgcttgct
     1081 gcgaagtcat cgaccacacg ctggacggcg acgttctgtg gaccgtggtt cgcgtcaccg
     1141 ccaggcaggc gggcgtcatg ggcctcgtgg ccggtgagtc cgtctgctac atcggctgcc
     1201 atctgctgga aagctcgggc ggcgattggg gctacaagtc cctcgatgag tccgtgcatc
     1261 cgtactacta ctcgtgcccg ctgcgctatc tcgacatggc gccggtgcaa agccccgagt
     1321 ggcgcgagcg ggttcaccgc ttccacgcgg ggcgcgctgt ctagcggcac gcatcttcct
     1381 tcacccaacc ggggcgagca tggccccgcg ggctgtggtt gctccttcat ttccaagagg
     1441 acatcaccat gcctgcacct tcttccgcga aaccgctgta ccgcatcgac gaatgccccg
     1501 acctcatggc cgacggttgt gtcggtgacg agcagggcaa tctcgtcttt ctctcaatct
     1561 gggcgcgtga caccgccgtt caggagttcc tcgctcgcct gaccctcggc cgggatgaac
     1621 agggactgga tcagttccac gtcatcaccg agcagggcgc atccatcccg gtct
//
LOCUS       DAORNX010000579         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51692_length_1673_cov_6.145859, whole genome shotgun sequence.
ACCESSION   DAORNX010000579 DAORNX010000000
VERSION     DAORNX010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51692_length_1673_cov_6.145859"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="PLO65_16890"
     CDS             <1..1540
                     /locus_tag="PLO65_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRD29974.1"
                     /translation="QVGEAPARARRASPHPLVASASDPTPAGRGKAFVDFQNDVTAKD
                     VRLAVREGFRSIEHIKRYTTTGMATDQGRTSNLNALSIASDALGTPVPQVGLTTFRPP
                     FTPVTFGALASFSRDGDLDPLRLTPLHDWAAGRGAVFEDVGLWRRARYFPRTGETMRE
                     AVARECLATRRGVGLFDASTLGKIEVVGPDAAEFLNRMYTGSWSKLAVGRCKYGVMLR
                     EDGFIFDDGVIGRMAPDRFHVTTTTGGAARVLAHMEDYLQTEFPDLAVWLTSTTEQWA
                     VIAVNGPRARDVVATVVEGIDLSNAAFPHMSVAEARLGDTPIRLFRVSFTGELGFEIN
                     VPASRAAMAWQAVLDAGAAHDICPYGTEAMHVLRAEKGYIIIGQDTDGTVTPDDAGLN
                     WAVGKTKPDFVGQRSLRRPDMLESSRKQLVGLLPVDPNIVLDEGAQILAGETVGEPGS
                     SLGHVTSSYWSSTLGRSMALGLVASGRRRLGHTLLSSHGGRFVAVTVGPTVFHDASGE
                     RLNG"
BASE COUNT          236 a          605 c          571 g          261 t
ORIGIN      
        1 ccaggtcggg gaggcgcccg cccgcgccag gcgcgcctcg ccgcacccac tggtcgccag
       61 cgcgtcggac ccgactccgg cagggcgcgg caaggcgttc gtcgacttcc agaacgacgt
      121 cacggccaag gatgtgcggc tcgccgtccg ggagggtttc cggtcgattg agcacatcaa
      181 gcgctacacc acgaccggca tggccaccga ccagggccga acgtcgaacc tgaacgccct
      241 gtcgatcgcc tcggacgccc tcgggacgcc cgtcccgcag gtcgggctga ccacgttccg
      301 ccctccgttc acgcccgtga catttggcgc cctggccagt ttctcgcgcg atggcgacct
      361 ggatccccta cgcctgacgc cgctgcacga ctgggcggcg ggtcgcggcg ctgtgttcga
      421 ggatgtcggc ctgtggcggc gggcgaggta cttcccgcgg acgggcgaga ccatgcgcga
      481 ggcggtcgcg cgcgaatgtc tggccacccg tcggggcgtc ggcctgttcg acgcctccac
      541 cctgggcaag atcgaggtcg ttggtcccga cgcggccgag ttcctgaacc ggatgtacac
      601 ggggtcgtgg agcaagctcg ccgtgggccg ctgcaagtac ggcgtgatgc tacgcgagga
      661 cggcttcatc ttcgatgatg gcgtcatcgg acggatggcg ccggaccgct tccatgtcac
      721 caccacgacc ggcggcgccg cccgcgtgct ggcccatatg gaagactatc tccagaccga
      781 gtttcccgac ctggccgtct ggctgacctc gaccacagag cagtgggcgg tcatcgccgt
      841 gaacggcccc cgggcccgcg acgtcgtcgc aacggttgtc gagggcatcg atctctcgaa
      901 cgccgccttc ccgcacatga gcgtcgccga ggcccggctc ggcgatacgc cgatccggct
      961 gttccgcgtc agcttcaccg gcgagctcgg cttcgagatc aatgttccgg cgtcccgcgc
     1021 cgccatggcc tggcaggcgg tcctggacgc cggggccgcc cacgacatct gtccctacgg
     1081 cacggaggcg atgcacgtgc tgcgcgctga aaagggctac atcatcatcg gtcaggacac
     1141 cgacgggacg gtaaccccgg acgacgccgg tctgaactgg gcggtgggca agaccaaacc
     1201 cgatttcgtc ggccagcgat ccctgcgccg gccggacatg cttgagtcct cgcgcaaaca
     1261 gctcgttgga ctgttgcccg tcgacccgaa catcgtgctc gacgaaggcg cgcagatcct
     1321 cgccggagag acggtcggcg agcccggctc cagtctcggc catgtcacat cgtcctactg
     1381 gagcagcacg ctcggccggt cgatggcgct gggcctggtg gcgtccggcc gccgccgact
     1441 gggccatacc ctgctgtcgt cccatggcgg ccggttcgtc gcggtcaccg tcgggccgac
     1501 ggtgttccac gacgccagcg gggagcgcct gaatggctga ggctccatcc atcccggcgc
     1561 cgcggctcgc tcacgccctt tgcgcggtcg agccggggcc gccctgctct cgactgtcgg
     1621 tccgcgcgcc ttcgacggcg ctgggcgccg tcgccgcggc ggccaaggcc ggc
//
LOCUS       DAORNX010000580         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51765_length_1671_cov_9.316832, whole genome shotgun sequence.
ACCESSION   DAORNX010000580 DAORNX010000000
VERSION     DAORNX010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51765_length_1671_cov_9.316832"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="PLO65_16895"
     CDS             complement(<1..119)
                     /locus_tag="PLO65_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29975.1"
                     /translation="MKPRHQVSRAAIELIKKFEGYRQRAAQLPDGRWTIGYGHT"
     gene            complement(301..>1671)
                     /gene="mgtE"
                     /locus_tag="PLO65_16900"
     CDS             complement(301..>1671)
                     /gene="mgtE"
                     /locus_tag="PLO65_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRD29976.1"
                     /translation="GDDYALNPEYVDMVIDAADRGDAERLRELVGALRSEDVADLMGF
                     LNADYREEIIPCLDSETLAEVLSELDDNLREEVLDSVHPTALARALEELDSDDAADVV
                     DDLEGEKRAQVLAAMDADDRAAIESSLAYEDETAGRLMQREVLAAPQFWTVGQTIDHV
                     RGAGDELPELFFDIYIVDPAWRPIGAAPISQLLRARRETTLAEIMEPVTEITVDMDQE
                     EVAYIFDKYHLISAPVIDPGGRLVGQITVDDIIGVIQEESEEDILALAGVSDAGRDAG
                     IIDIVRARLPWLMLNLLTATLAVSGVALFQDQIAKVVALAVLMPIVSSLGGNAGTQTL
                     AVAVRALASRELSAANARRIVLRELAVGLTNGLSLAAVMGVAVFAVYQLLDHDTATNG
                     RLAITVALALVVNIFTAALGGIFAPLLLERMGRDPAVSSSIFVTFLTDFTGFFAVLLI
                     ASLLFA"
BASE COUNT          276 a          560 c          576 g          259 t
ORIGIN      
        1 gtgtggccgt agccgatcgt ccagcgcccg tcggggagct gggcggccct ttgtcgatag
       61 ccctcgaact tcttgatcag ttcaatggcg gctcgggaga cctggtggcg gggtttcatc
      121 ggccttcttg atccgaaatg agacagacac ggctgcgttc aaccccactc ccgcaaaagg
      181 gatgccgatt ggccgacggg aacgcctgtc aggagaatcg gcgcagcccg aacagggcct
      241 attgcccgcg aaaccggaca ttcgcgtgac cgcccggccg gaccatcctg cctgaccccg
      301 tcaggcgaac agaagcgagg cgatcagcag aaccgcgaag aacccggtga aatcggtgag
      361 gaaggtcacg aagatcgagg acgacacggc cgggtcgcga cccatccgct ccagcagcag
      421 cggtgcaaag atcccgccaa gcgccgcggt gaagatgttg accaccaggg ccagggccac
      481 ggtgatcgcc agccgcccgt tggtcgccgt gtcgtggtcg agcaactggt agacggcgaa
      541 caccgccacg cccatcacgg cggccaggct caggccgttg gtcaggccca ccgccagctc
      601 gcgcagcaca atgcggcggg cgtttgccgc gctcagttca cggctggcca gggcgcgcac
      661 ggccacggcc agggtctggg tgccggcgtt gccgcccagc gacgagacga tcggcatcag
      721 cactgccagg gcgacgacct tggcgatctg gtcctggaac agggccacgc cgctgaccgc
      781 cagggtcgcg gtcagcaggt tgagcatcag ccagggcagg cgcgcgcgga cgatgtcgat
      841 gatgccggcg tcgcggccgg cgtcggacac gccggccagg gccaggatat cctcctccga
      901 ctcttcctgg atgacgccga tgatgtcgtc gacggtgatc tggccgacca gccgcccgcc
      961 cggatcgatc accggcgcac tgatcaggtg atacttgtcg aagatgtagg cgacctcttc
     1021 ctggtccatg tcgacggtga tctcggtcac cggctccatg atctcggcca gcgtggtctc
     1081 gcgcctggcc cgcagcagct ggctgatcgg ggccgcgccg atcgggcgcc aggccgggtc
     1141 gacgatgtag atatcgaaga acagctcggg cagctcgtcg ccggcgccgc ggacgtggtc
     1201 gatggtctgg ccaacggtcc agaactgggg tgcggccagc acctcgcgct gcatcaggcg
     1261 gccggcggtc tcgtcctcgt aggccaggct gctctcgatg gccgcccggt cgtcggcgtc
     1321 catcgccgcc agcacctggg cgcgcttctc gccctccagg tcgtcgacga cgtcggccgc
     1381 gtcgtcggag tcgagctctt ccagggcgcg ggccagggcc gtcggatgaa ccgagtcgag
     1441 gacctcctcg cgcaggttgt cgtccagttc ggacagcacc tcggcgaggg tctccgaatc
     1501 gagacaggga atgatctcct cgcggtagtc cgcgttgagg aagcccatca ggtcggccac
     1561 gtcctccgac cgcagggcgc cgaccagctc acgcagccgc tcggcgtcgc cgcggtcggc
     1621 ggcgtcgatg accatgtcga cgtattcggg attgagggcg tagtcgtcgc c
//
LOCUS       DAORNX010000581         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51766_length_1671_cov_7.286510, whole genome shotgun sequence.
ACCESSION   DAORNX010000581 DAORNX010000000
VERSION     DAORNX010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51766_length_1671_cov_7.286510"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLO65_16905"
     CDS             complement(<1..298)
                     /locus_tag="PLO65_16905"
                     /inference="COORDINATES: protein motif:HMM:NF021025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HRD29977.1"
                     /translation="MTFSLTRLTPYAALLALGACASGPAPGTGALTPNALETWSSQVR
                     VVGEPEEIRLAPHATGLSGTQARALAEFHARWTLAEGGVITVAAPTGAQGSGAYR"
     gene            complement(303..>1671)
                     /locus_tag="PLO65_16910"
     CDS             complement(303..>1671)
                     /locus_tag="PLO65_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRD29978.1"
                     /translation="LAGGPGGGPATVRVDLGSGGVTRDLTLPRGKSAVIELPVDVRDI
                     LITNPAVADAVLRSPRQIYVLGMTSGQTDAILFDASGRRILSLNIRVDADFGALAETI
                     NRVLPGARVRVEPIGEAVILTGMVANASDADKAGQIAKQYVAKPEQVLNMLSIAGKDQ
                     VMLRVRVVEVQRSVIKQLGVNLNAVLQQGTGNITLNQAAAFSINGTLGGGATIGGTYT
                     SPTTPADVLTGSIRAFERVGLVRTLAEPNLTAVSGEAAKFLAGGEYPVPVNQDSSNGS
                     VTVEYKPFGVGLAFTPVVLSGGRISLKISTEISELTTQGAFTLGSATSGTSLTIPALT
                     VRRAETSIELPSGGSMMIAGLIQQTTRENLDALPGVGSVPIIGSLFRSRDFLSGDTEM
                     VVIVTAYVVDPQNPGAFQTPADGLQFASDAETILFGKLNKAVGAPPGANAGRAYQGPI
                     GYVIE"
BASE COUNT          242 a          553 c          624 g          252 t
ORIGIN      
        1 cgcggtaggc gccggacccc tgggccccgg tcggggcggc cacggtgatc accccgccct
       61 cggcgagagt ccagcgggca tggaattccg ccagggcgcg ggcctgggtc cccgaaaggc
      121 cggtggcgtg cggggccagc cggatctcct cgggctcgcc gacgacacgc acctgcgacg
      181 accaggtctc gagcgcgttg ggcgtgagag cgccggtccc cggggccggc ccggaagcgc
      241 aggcgccgag cgccagcagc gcggcgtagg gcgtcagtcg ggtgaggctg aaggtcatgg
      301 cgttactcga tcacgtaacc gatggggccc tggtaggcgc gtccggcgtt ggcgccgggc
      361 ggcgcgccga cggccttgtt cagcttgccg aacaggatgg tctcggcgtc gctggcgaac
      421 tgcaggccgt ccgccggggt ctggaaggct ccgggattct gcggatcgac cacataggcg
      481 gtgacgatga cgaccatctc cgtgtcgccc gacagaaagt cgcgcgagcg gaacagcgag
      541 ccgatgatcg gcaccgatcc cacgccgggc agggcgtcga ggttctcgcg ggtggtctgc
      601 tggatcaggc cggcgatcat catcgacccg ccggagggca gctcgatcga ggtctcggcc
      661 cgccggacgg tcagggccgg gatggtcagg ctggtgccgc tggtcgcgct gccgagggtg
      721 aaggcgccct gggtggtcag ctcggaaatc tcggtcgaga tcttcagcga gattcgtccg
      781 cccgacagca caaccggcgt gaaggcaagg cccacgccga agggcttgta ttcaacggtc
      841 accgatccgt tgctggaatc ctggttgacc gggaccggat actccccgcc cgcgaggaac
      901 ttggccgcct cgcccgagac ggcggtcagg ttcggttcgg ccagggtgcg caccagaccg
      961 acgcgctcga aggcgcggat cgatccggtc agcacatcgg ccggggtggt cgggctggtg
     1021 taggtcccgc caatcgtcgc tccgccgccc agcgtgccgt tgatgctgaa ggcagccgcc
     1081 tggttgaggg tgatgttgcc cgtgccctgc tgcaggacgg cgttgaggtt gacgcccagc
     1141 tgcttgatga cgctgcgctg gacctcgacc acgcgcacgc gcagcatcac ctggtccttg
     1201 ccggcgatgc tgagcatgtt gagcacctgc tcgggcttgg ccacgtactg cttggcgatc
     1261 tggccggcct tgtcggcgtc gctcgcgttg gcgaccatgc cggtgaggat caccgcctcg
     1321 ccgatcggct cgacccggac ccgggccccg ggaaggacgc ggttgatggt ttccgccagc
     1381 gctccgaaat cggcgtcgac gcggatgttg agcgacagga tgcggcggcc ggaggcgtcg
     1441 aacaggatcg cgtcggtctg gcccgacgtc atgcccagca catagatctg gcgcggcgaa
     1501 cgcagcacgg cgtcggccac cgccggattg gtgatcagga tgtcgcgcac gtcgaccggc
     1561 agctcgatca ccgccgactt gccgcgcggc agggtcaggt cgcgcgtgac gccgcccgat
     1621 cccagatcga cgcgcacggt ggccgggccg ccgccggggc cgccggccag g
//
LOCUS       DAORNX010000582         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51767_length_1671_cov_4.696782, whole genome shotgun sequence.
ACCESSION   DAORNX010000582 DAORNX010000000
VERSION     DAORNX010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51767_length_1671_cov_4.696782"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLO65_16915"
     CDS             complement(<1..297)
                     /locus_tag="PLO65_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRD29979.1"
                     /translation="MNAPTPVLELAPALVVLPGARSAVADGAGAKDLRPPYAREMLEH
                     GPVLLAHAGMTARRLGMNAPGRSRSIFDALELFAFVRPAKFTAPSAVGLAQALGL"
     gene            403..1176
                     /locus_tag="PLO65_16920"
     CDS             403..1176
                     /locus_tag="PLO65_16920"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRD29980.1"
                     /translation="MRNFLIAAAITVLSAGGALAQSADTAQIVVSGSGEAEAPADWAA
                     VSIELVGEGPTAVVAVNALTGKQTQLEARIKSLAKASSVGIQTGDLTVSAIRGADCED
                     ARGYRPRPVLSEGVCTVKGYAALMKIEVRITPASEAGNVASLATELGALDATLSGYGL
                     DHPELLAEQATRDAIEDARFQAMTIARASNVRLGPVVRIQMPSSYADPELDVAVADAA
                     ASDAAAAPLLARPPAATIDITPPPVKAAARLSVIYSVLP"
     gene            complement(1173..>1671)
                     /locus_tag="PLO65_16925"
     CDS             complement(1173..>1671)
                     /locus_tag="PLO65_16925"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD29981.1"
                     /translation="DGEGTPKDLKKALYWFRKSAARGKAEAMNELGCAYSKGAGVALD
                     YSAALGWFRRAAARGDIIAMLNIGVAYDLGQGVPIDDAEAVRWYRRAAEGGDPTAALY
                     LASMYERAEGVRLDFAEAARWYGRAAGSEDADERAEARAGLDRVGEGVLPIAPPRDAT
                     SLTAT"
BASE COUNT          261 a          589 c          579 g          242 t
ORIGIN      
        1 caggcccagc gcctgggcaa gccccacggc cgagggggcg gtgaacttcg ccggccgcac
       61 gaacgcgaac agctccaggg cgtcgaagat cgaccgggac cggccggggg cgttcatgcc
      121 gagccggcgg gccgtcatac ccgcatgggc cagcagcacc ggcccgtgct caagcatctc
      181 gcgggcgtag ggcggccgca ggtcctttgc ccccgccccg tcggccacgg ccgaccgcgc
      241 gccgggcagg acgacgaggg cgggcgcgag ctcgaggacg ggagtcgggg cgttcacgcc
      301 caaggtctag cgtggaaact tggaaaacga aacgggaaca acaactcgct tgcgggcgcg
      361 cacgccatac gtgcaagctc cactcgcaaa attgggggga ccatgcgcaa tttcctgatc
      421 gccgcagcca tcaccgttct gtctgccgga ggagcgctcg cccagtccgc cgacaccgcc
      481 cagatcgttg tcagtggttc gggcgaggcc gaagcgccgg cggattgggc cgcagtttcg
      541 attgagcttg ttggcgaagg cccaaccgcc gtcgttgcgg tcaacgcctt gaccggcaag
      601 cagacccagc tggaggcgag gatcaagtca cttgccaagg cctcctcggt aggcatccag
      661 accggcgacc tcaccgtctc ggccatccgg ggggcggact gcgaagatgc gcggggctat
      721 cgaccgcgtc ccgtgctttc tgaaggcgtc tgcaccgtga agggctacgc ggcgctgatg
      781 aagatcgaag tgcgcatcac gcccgcctcg gaggcgggca atgtcgcatc gctggccacg
      841 gagctgggcg cgctggacgc gacgctttca gggtacgggc ttgatcatcc ggaattgttg
      901 gctgaacagg ccacacggga cgcaatcgaa gacgcgcgat ttcaagcgat gacgatcgca
      961 agagcctcca acgttcgcct ggggccagtc gtgcgcatcc agatgcccag cagctatgcc
     1021 gacccggagc tcgacgtcgc agtggccgac gccgccgcat cggacgcggc ggccgccccg
     1081 ctgctggcaa ggccgcctgc ggctacaatc gacatcacgc cgcctccggt gaaggctgcg
     1141 gcgcgcctga gcgtgatcta ctcggttctg ccttaggtcg ccgtcaggct ggtcgcatcc
     1201 ctgggcgggg cgatcggcag gacgccctcg ccgacacggt ccagcccggc ccgcgcctcg
     1261 gcccgttcgt cggcgtcctc ggagccggcg gcccggccgt accagcgggc ggcctcggcg
     1321 aagtccagcc tgaccccctc ggcccgttcg tacatgctgg ccaggtagag ggccgccgtc
     1381 gggtcgccgc cctcggccgc cctgcggtac cagcgcaccg cctcggcgtc gtcgatcggc
     1441 acgccctggc cgaggtcata ggccacgccg atgttcagca tggcgatgat gtcgccgcga
     1501 gcggcggcgc gccggaacca gccgagggcc gcgctgtagt cgagcgccac gccggcgccc
     1561 ttggagtagg cgcagccgag ctcgttcatc gcctccgcct tgccccgggc ggccgacttc
     1621 cggaaccagt agagggcctt cttcaggtcc ttcggcgtcc cttcgccgtc g
//
LOCUS       DAORNX010000583         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51828_length_1669_cov_6.982652, whole genome shotgun sequence.
ACCESSION   DAORNX010000583 DAORNX010000000
VERSION     DAORNX010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51828_length_1669_cov_6.982652"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLO65_16930"
     CDS             <1..318
                     /locus_tag="PLO65_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD29982.1"
                     /translation="DLRHVGGHHDRLALAEGAASETAPFEQALAADPDDHAARFALAG
                     ALAGQGRLAAAADQLLMIIARDQTWNDGAARKQLLTVFEAAGLNSEIARQGRRRLSSI
                     LFS"
     gene            327..980
                     /locus_tag="PLO65_16935"
     CDS             327..980
                     /locus_tag="PLO65_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRD29983.1"
                     /translation="MATGFRKAIDLPQAVPVFPLDGALLLPGGQLPLNIFEPRYLNMI
                     DDAMAGDRLIGMIQTSGGDPGRPELAPVGCVGKITSFAETPDGRYLITLTGLCRFALG
                     EELPVGSPYRQVRARFAPYEDDLRHHEAPPEIDRPRFLEALGRYLDHRGLGIDWDTAR
                     QAPAGALVNSLSMALPFDPAEKQALLEAVTIADRAEALFALLEIDSAGGDDEPRSMQ"
     gene            991..1191
                     /locus_tag="PLO65_16940"
     CDS             991..1191
                     /locus_tag="PLO65_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRD29984.1"
                     /translation="MSETAPSPVSDVDPRLLEILVCPVTRTPLTLDRARGELVSKAAK
                     LAYPIRDGVPIMLPEEARALEE"
     gene            complement(1274..>1669)
                     /locus_tag="PLO65_16945"
     CDS             complement(1274..>1669)
                     /locus_tag="PLO65_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD29985.1"
                     /translation="IAERLCAPRIALLGDAAHGVHPIAGQGLNLGLKGAAALAEVLVE
                     AARLGEDIGAEAVLERYAGWRRFDTNTLAAGMDAFVWLFSNDNPLVRLARGVGMAAVN
                     RIGPARRFFMHEAGGAVGDLPRLLRGEAL"
BASE COUNT          248 a          618 c          569 g          234 t
ORIGIN      
        1 gatcttcggc acgtcggcgg tcatcacgat cgtctcgccc tggccgaggg cgcggcctcc
       61 gagaccgcgc cgttcgaaca ggcgctggcc gccgaccccg acgatcatgc ggcgcgtttc
      121 gccctggccg gcgccctggc cgggcaggga cgcctcgcgg cggcggcgga ccagctgctg
      181 atgatcatcg cccgcgacca gacctggaac gacggcgccg cacgcaaaca actgctgacg
      241 gtgttcgagg ccgccgggct gaactccgag atcgccaggc agggccggcg ccggctgtcc
      301 tcgatcctgt tcagctaggc gacgccatgg ccacgggatt tcgcaaggcg atcgatctgc
      361 cccaggccgt tccggtgttc cccctggacg gggcgctgct gctgccgggc ggtcagctgc
      421 cgctgaacat cttcgagccg cgctacctga acatgatcga tgacgccatg gccggggacc
      481 gcctgatcgg catgatccag acctccggcg gcgatcccgg gcggccggaa ctggcgccgg
      541 tcggctgcgt gggcaagatc accagtttcg ccgaaacccc ggacggccgc tacctgatca
      601 ccctgaccgg actctgtcgc ttcgcgctgg gcgaggagct gccggtcggc tcgccctatc
      661 gtcaggtccg ggcccggttc gcgccctacg aggacgacct gcgccaccac gaggcgccgc
      721 cggaaatcga ccgaccgcga ttcctcgagg ccctcggccg ctatctcgac caccggggtc
      781 tgggcatcga ctgggacacg gcgcggcagg cccccgcggg cgcgctggtc aacagcctgt
      841 cgatggccct gcccttcgat ccggccgaaa agcaggcgct gctggaggcc gtgaccatcg
      901 ccgatcgcgc cgaggccctg ttcgccctgc tggagatcga ctccgccggc ggcgatgacg
      961 aaccccggtc gatgcagtag aagcggcccc atgagcgaaa ccgccccctc ccccgtcagc
     1021 gacgtcgatc cgcgcctgct ggagatcctc gtctgccccg tgacccgcac gcccctgacc
     1081 ctggaccgcg ccaggggcga gctggtcagc aaggcggcga agctggccta tccgatccgt
     1141 gacggggtgc cgatcatgct gccggaggag gcgcgggcgc tggaggagta gggtgtaggg
     1201 aatagggagt agttgagcgg cgtcgttctg gccgcggcgc ttcagccccg atctctatgc
     1261 tactccctat tccctaaagg gcctcgcccc gcagcagccg cggcaggtcg ccgacggcgc
     1321 cgccggcctc gtgcatgaag aaccggcgcg caggaccgat gcggttgacc gccgccatgc
     1381 ccacgccacg cgccagccgg accagcggat tgtcattgga aaacagccag acgaaagcgt
     1441 ccatgccggc ggccagggta ttggtgtcga accgccgcca gccggcgtag cgctcgagca
     1501 ccgcctccgc gccgatgtcc tcgcccagcc gcgcggcctc gaccagcacc tcggccagcg
     1561 ccgccgcccc cttcagcccg aggttcaggc cctggccggc gatcggatgg acgccgtgcg
     1621 ccgcgtcgcc gagcagggcg atgcgcggcg cgcacagccg ctcggcgat
//
LOCUS       DAORNX010000584         1668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_51870_length_1668_cov_4.561066, whole genome shotgun sequence.
ACCESSION   DAORNX010000584 DAORNX010000000
VERSION     DAORNX010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51870_length_1668_cov_4.561066"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1668
                     /locus_tag="PLO65_16950"
     CDS             136..>1668
                     /locus_tag="PLO65_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD29986.1"
                     /translation="MHRPSVPVFSADATPNLLSPARRLRRVVLIGSFPPRRCGIATFT
                     CDVHDALKTAAPGLDCQVVAMTDEGGHYAYDRDVMFEVRQNRAADYLEAARRINAVEP
                     DAVCVQHEFGLFGGPAGEHLMLMLDAVRAPVTTTLHTVLTHPDIDQRRVFDRLIRRSA
                     RIIVMAERGRRILTEVWGVAPEKIVVTPHGAPDRPLIDTAGPKARLGLDGREVMLTFG
                     LLSPGKGLESMIRAMPDIVRARPDALYVILGATHPHLVARDGEVYREGLFALARDLGV
                     GDHVRFHNQYLDTAQMLDWLAAADLYVTPYLNEAQITSGTLSYAVALGKAVISTPYWH
                     AQELLADDRGVLTPFNDPDALATAAITLLADPERLAALRRRAWEAGRDTLWSRLADRY
                     LEVFASVRVSRPPQRAANAEPRSFAPDALPRPSLAGVRRMTDAVGILQHSMFDVPDRN
                     HGYCVDDNARALLLMHRLRALGLHSAETEALAETYSAFVHHAWNDGAGRFRNFMAFDR
                     TWL"
BASE COUNT          225 a          651 c          528 g          264 t
ORIGIN      
        1 gcccgggccg ccttgtcgtc cgcgccggtt gaggggccgg tcgccatgcc gcagccgccc
       61 cgcgcatcca aaccggaccg aatggcgttg cgctatcaac gggcgtcgga caggccgtcg
      121 ccgccaggat ccgccatgca ccgcccgtct gttcccgtgt tttccgccga cgccactccg
      181 aacctgctgt cccccgcccg ccgccttcgc cgggtggtgc tgatcggcag cttccccccg
      241 cgccgttgcg gcatcgccac cttcacctgc gacgtccatg acgccctcaa gacggcggcc
      301 cccggcctcg attgccaggt cgtggcgatg accgacgagg gtggccacta cgcctacgac
      361 cgggatgtca tgttcgaggt tcgccagaac cgcgccgccg actacctgga ggcggcccgg
      421 cggatcaacg cggtcgagcc tgacgcggtc tgcgtccagc acgagttcgg cctgttcgga
      481 ggtcccgccg gcgagcatct gatgctgatg ctcgacgccg ttcgcgcacc ggtgacgacc
      541 accctgcaca ccgtgctgac ccacccggac atcgaccagc ggcgcgtctt cgatcgtctg
      601 atccggcgct cggcgcggat catcgtcatg gctgaacggg gccggcgaat cctgaccgaa
      661 gtctggggcg tcgcgcccga gaagatcgtc gtcacgcccc atggcgcgcc cgaccggccg
      721 ctgatcgata ccgccggccc gaaggcccgg ctgggactcg atggccgcga ggtgatgctg
      781 accttcggcc tgctgtcgcc gggcaagggg ctggagtcga tgatccgcgc catgcccgat
      841 atcgtgcgcg cccggcccga cgccctgtat gtcatcctcg gcgccaccca tccgcacctg
      901 gtcgcccgcg acggcgaggt ctaccgcgag ggcctgttcg ccctggcccg ggatctcggg
      961 gtcggcgacc atgtgcgctt tcacaaccag tatctcgaca ccgcccagat gctcgactgg
     1021 ctggccgcgg ccgatctcta tgtcacgccc tacctcaacg aggcgcagat cacctcgggg
     1081 acgctgtcct acgccgtggc cctcggcaag gcggtcatct ccacccccta ctggcatgcc
     1141 caggagctgc tggccgatga ccgtggcgtt ctgaccccct tcaacgaccc cgacgccctg
     1201 gccacggcgg ccataaccct gctggccgat cccgagcgcc tggcggcgtt gcgccggcgg
     1261 gcctgggagg ccggtcgcga taccctgtgg agccgcctgg ccgaccgcta tctcgaggtc
     1321 ttcgcctcgg tgcgcgtatc gcggcccccg cagcgcgcgg ccaacgccga gccgcgcagc
     1381 ttcgcccccg acgccctgcc ccgcccctcg ctggccgggg tccgccggat gaccgacgcc
     1441 gtcggcatcc tgcaacacag catgttcgac gtgccggacc gcaaccacgg ctactgcgtc
     1501 gatgacaacg ccagggcgct gctgctgatg catcgcctgc gcgccctcgg cctgcactcc
     1561 gccgagacgg aggccctcgc cgagacctat tccgcgtttg tccaccacgc ctggaacgac
     1621 ggcgccggcc ggttccgcaa cttcatggcc tttgaccgga cctggctc
//
LOCUS       DAORNX010000585         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_52045_length_1664_cov_6.047234, whole genome shotgun sequence.
ACCESSION   DAORNX010000585 DAORNX010000000
VERSION     DAORNX010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52045_length_1664_cov_6.047234"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLO65_16955"
     CDS             <1..520
                     /locus_tag="PLO65_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD29987.1"
                     /translation="EAAPEVRPTPLRAALEAAAEDAGLSESGVRLETRIGAGEAVVAD
                     PDQLHRILVNLLRNAREAIQDTDPDRAGQVEATLNRRDGLSILRLADDGPGLPDRAMA
                     NLFQPFSGSARRGGTGLGLAISRELAQAHGGDLTLVSSSADGSVFDLILPGVVEAPAP
                     KPRRPRRKKTEV"
     gene            complement(517..>1276)
                     /locus_tag="PLO65_16960"
     CDS             complement(517..>1276)
                     /locus_tag="PLO65_16960"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD29988.1"
                     /translation="AFGRLQRLMYADRDRYVGDIEVPIAGLLKPAYVDARAALIGDRA
                     GPVPAFGNPEGAGVRGPDRTVEPGGTSHFVVVDAKGNVVSMTTTVESIFGSGRMVAGF
                     FLNNQLTDFSFSPTDKDGVPAANAVAPGKRPRSSMSPVIVLDRQGRLVAALGSPGGNS
                     IISYNLKALVGILDWKLAPAQAFGLPNLVARGDRFSSEPDRYAPGVVEGLKARGVVFT
                     NTAGENSGLHGVVVTPGGLVGGADPRREGLAKGF"
     assembly_gap    1277..1286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1287..>1664)
                     /locus_tag="PLO65_16965"
     CDS             complement(<1287..>1664)
                     /locus_tag="PLO65_16965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD29989.1"
                     /translation="DSRRYFARPDGTRVQAGDRLKNPDYATTVRRLAAEGPDALYEGP
                     IAQAIVARLHEGDLPGTMTLADIRAYRPRAAPALCRPYRIYVVCTTNPPSSGVGLLQG
                     LMLLERTDIAERGPTDPLAWVRL"
BASE COUNT          237 a          588 c          615 g          214 t
ORIGIN      
        1 cgaggccgcg cccgaggtcc ggcccacgcc ccttcgcgcg gcgctcgagg ccgcggccga
       61 ggacgcgggc ctcagcgaaa gcggcgtccg gctggagacg cgcatcggag cgggcgaggc
      121 cgtggtcgcc gaccccgacc agctgcaccg gattctcgtc aacctgctgc gcaacgcccg
      181 cgaggcgatc caggacacgg acccggaccg ggccggccag gtcgaggcga cgctgaaccg
      241 gcgtgacggg ctcagcatcc tgcggctggc cgacgacggt ccgggcctgc cggaccgggc
      301 catggccaac ctgttccagc ctttctccgg ttccgcccgg cgcggcggca cgggcctagg
      361 cctggccatc tcgcgcgagc tggcccaggc ccatggcggt gacctgaccc tggtcagttc
      421 gtcggcggat ggttcggtgt tcgacctgat cctgccgggc gtggtcgagg ctccggcgcc
      481 gaagccacga cggccgcgaa ggaagaagac ggaagtctag aaccccttcg ccaaaccttc
      541 ccgccgcgga tcggccccgc cgaccaggcc gccgggcgtg accaccacgc catgcaggcc
      601 ggagttctcg ccggcggtgt tggtgaacac cacgccgcgc gccttgaggc cctcgaccac
      661 gccgggcgca taccggtccg gctcgctgga gaaacggtcg ccacgggcca ccaggttggg
      721 caagccaaag gcctgcgccg gggccagctt ccagtcgagg atcccgacca gggccttgag
      781 attgtaggag atgatcgagt tgccgcccgg cgagcccagg gccgccacca gccgtccctg
      841 gcggtcgagc acgatgaccg gcgacatcga cgagcgcggc cgcttgcccg gcgccacggc
      901 gttggcggcg gggacgccgt ccttgtcggt cggcgagaag ctgaagtcgg tcagctggtt
      961 gttgaggaag aagccggcga ccatccgtcc cgagccgaag atgctctcga ccgtggtggt
     1021 catcgagacc acgttgccct tcgcgtcgac gaccacgaag tgggaggtgc cgcccggctc
     1081 gaccgtgcgg tctggacccc ggacgccggc gccctcgggg ttgccgaagg ccggaaccgg
     1141 tccggcccgg tcgccgatca gggccgcgcg ggcgtcgaca taggccggct tgagcaggcc
     1201 ggcgatcggc acctcaatgt cgccgacata gcggtcgcgg tcggcgtaca tcagccgctg
     1261 caaccggccg aaggcgnnnn nnnnnnccag ccgcacccag gcgagcgggt cggtcgggcc
     1321 gcgttcggcg atatcggtcc gctccagcag catcaggccc tgcagcagcc ccacgccgct
     1381 cgacggcggg ttggtcgtgc agaccacata gatccggtag ggccggcaca gggccggcgc
     1441 cgcgcgcggg cgataggcgc ggatgtcggc cagggtcatg gtcccgggca ggtccccctc
     1501 gtgcagccgg gcgacgatgg cctgggcgat cggcccctcg tagagggcgt cgggcccctc
     1561 ggcggcgagc cggcggacgg tggtcgcgta gtcggggttc ttcagccggt cgccggcctg
     1621 aacgcgggtc ccgtcgggcc tggcgaaata gcggcgactg tcgg
//
LOCUS       DAORNX010000586         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_52047_length_1664_cov_5.799876, whole genome shotgun sequence.
ACCESSION   DAORNX010000586 DAORNX010000000
VERSION     DAORNX010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52047_length_1664_cov_5.799876"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..>1664)
                     /gene="xdhB"
                     /locus_tag="PLO65_16970"
     CDS             complement(48..>1664)
                     /gene="xdhB"
                     /locus_tag="PLO65_16970"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRD29990.1"
                     /translation="GGFGGKESQPALFAAAAALMATRTGRPAKLRLDRDDDMVMTGKR
                     HDFETAWTVGFDDDGRLAGAAFDLASRCGCSADLSLAINDRAMFHADNAYALRTAEIV
                     SHRFRTHTVSNTAFRGFGGPQGMMPVERAMDAIALHLGKDPLDVRKANLYGVAGDRTP
                     YGQTVEHSPALALIEQLEAGSDYRARRAAIRAWNAGNRWLKRGIALTPVKFGISFTAT
                     HLNQAAALVHVYTDGSVMLNHGGTEMGQGLYVKVAQVVADVFGVDLERVKITATVTDK
                     APNTSATAASSGSDLNGMAALDAALQIRARMAAVAGEAFGVTPDAVVFAGNAVQAGGQ
                     TMTFADLAKKTWFNRISLSAAGYYRTPKIHYDRAVHQGRPFLYYAWGAAVSEVIIDTL
                     TGEHRCLRTDILHDCGASLNPAIDLGQIEGGFVQGMGWLTTEELVFDDNGVLRTHAPS
                     TYKIPTASDRPPVLNIAFWDRPNAEPTIHRSKAVGEPPLMLAFSVFSALTQAVAAAAD
                     YRVMPDLDAPATPERILMAVDDVRRRAGLA"
BASE COUNT          223 a          561 c          628 g          252 t
ORIGIN      
        1 cagggactcg ccgcggcgga cggcggacag ggctgtcgcg gcccagctca cgccagcccc
       61 gcccggcgac gcacgtcgtc cacggccatc aggatgcgct cgggcgtggc cggggcgtcg
      121 aggtccggca tgaccctgta gtccgccgcg gccgccacgg cctgggtcag ggccgagaac
      181 accgagaagg ccagcatcag cggcggctcg cccacggcct tggaccgatg gatcgtcggc
      241 tcggcgttcg gccggtccca gaaggcgatg ttcaggaccg gcggccggtc cgacgccgtc
      301 gggatcttgt aggtcgaggg cgcgtgggtc ctcaggaccc cgttgtcgtc gaacaccagc
      361 tcctcggtgg tcagccagcc catgccctgg acgaagccgc cctcgatctg gcccaggtcg
      421 atggccgggt tcagcgaggc gccgcagtcg tgcaggatat ccgtgcgcag gcagcggtgc
      481 tcgccggtca gggtgtcgat gatcacctcc gacacggccg cgccccaggc gtagtagagg
      541 aacggccggc cctggtggac cgcgcggtcg tagtggatct tcggcgtgcg gtagtagccg
      601 gcggccgaca gcgagatgcg gttgaaccag gtcttcttcg ccaggtcggc gaaggtcatg
      661 gtctgtccgc ccgcctggac agcgttcccg gcgaacacga ccgcgtcagg cgtgacgccg
      721 aaggcctcac cagcgacggc ggccatgcgg gccctgatct gcagcgccgc gtccagcgcc
      781 gccatgccgt tgagatccga gccggacgag gcggcggtgg ccgaggtgtt gggcgccttg
      841 tcggtcaccg tggcggtgat cttcacccgc tcgaggtcga cgccgaatac gtcggcgacc
      901 acctgggcga ccttcacata caggccctgg cccatctccg tgccgccgtg gttcagcatc
      961 acgctgccgt cggtgtagac gtgcacgagg gccgcggcct ggttcaggtg ggtggccgtg
     1021 aagctgatgc cgaacttgac cggcgtcagg gcgatgccgc gcttgagcca gcggttcccg
     1081 gcgttccagg cgcggatcgc ggcgcggcgg gcgcggtagt cgctgccggc ctcaagctgt
     1141 tcgatcaggg ccagggccgg gctgtgctcg acggtctggc cgtagggcgt ccggtcgccg
     1201 gccacgccgt agaggttggc cttgcgcaca tcgagcgggt ccttgcccag gtgcagggcg
     1261 atggcgtcca tcgcgcgttc gaccggcatc attccctgcg gcccgccgaa gccgcggaag
     1321 gcggtgttcg acacggtgtg ggtgcggaac cggtgcgaga cgatctcggc cgtgcggagg
     1381 gcgtaggcgt tgtcggcgtg gaacatcgcc cggtcgttga tcgccagcga caggtcggcc
     1441 gagcagccgc agcggctggc caggtcgaag gccgcgccgg ccagccggcc gtcgtcgtcg
     1501 aagccgaccg tccaggccgt ctcgaagtcg tgccgcttgc cggtcatgac catgtcgtcg
     1561 tcgcggtcga gacgcagctt ggcggggcgg cccgtgcgcg tggccatgag ggcggcggcg
     1621 gccgcgaaca gggcaggctg gctctccttg ccgccgaagc cccc
//
LOCUS       DAORNX010000587         1654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_52414_length_1654_cov_6.621639, whole genome shotgun sequence.
ACCESSION   DAORNX010000587 DAORNX010000000
VERSION     DAORNX010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52414_length_1654_cov_6.621639"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLO65_16975"
     CDS             complement(<1..216)
                     /locus_tag="PLO65_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD29991.1"
                     /translation="MKVGVVGCGVGGMAAALALARRGHEVTLLEAFDAPRPLGSGLLL
                     QPTGLKALGSLGLEAMIRERGARVDRLD"
     gene            complement(223..1197)
                     /gene="cysK"
                     /locus_tag="PLO65_16980"
     CDS             complement(223..1197)
                     /gene="cysK"
                     /locus_tag="PLO65_16980"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRD29992.1"
                     /translation="MTSYDAARFKRAGRGKIYDSILDTVGDTPLVRLPRLSAELKPRA
                     EVLGKLEFFNPMNSVKDRIGVSMIEWLEAQGHLKPGGMIVEPTSGNTGIALAFVAAAK
                     GYRITLVMPESMSMERRKMLLILGARLELTPAEKGMRGAVARAQEILEEIPGSVMPQQ
                     FENEANPLIHRVSTAEEIWNDTAGSVDAVVSGVGTGGTITGVGQVLKARKPSIKMVAV
                     EPEASPVLSGGQPGAHKIQGIGAGFVPGILDRGVIDDIVKVGNDDAIAMARRCASVEG
                     IPVGISSGAALTAAFELASRAEYAGKVIVTIIPSFAERYLSTALFEGL"
     gene            complement(1268..>1654)
                     /locus_tag="PLO65_16985"
     CDS             complement(1268..>1654)
                     /locus_tag="PLO65_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRD29993.1"
                     /translation="TPEHLGRLRRAGEQGRAVGTAFRRMRGGVQRAEVRLDGVAGCLR
                     TPRGGSSRQTVIVAEPSLIRSRLLAPREGARLMGLPDSYRLPKGDTAAFHVIGDGVAV
                     PVVRWLAAHLLEPLLAESDAPALAAE"
BASE COUNT          239 a          589 c          586 g          240 t
ORIGIN      
        1 atcgagccgg tcgacgcggg ccccgcgctc gcggatcatc gcctccagac ccagggaccc
       61 gagcgccttc aggccggtcg gttgcagcag caatcccgat cccagggggc ggggcgcgtc
      121 aaaggcctcc agcagggtga cctcatggcc gcgccgggcg agggccaggg ccgccgccat
      181 gccgccgacc ccgcatccga cgaccccgac cttcaccggc ggctacaggc cttcgaacag
      241 ggcggtcgac aggtagcgtt cggcgaagct ggggatgatg gtgacgatca ccttgcccgc
      301 gtactccgcc cgcgaggcca gctcgaaggc cgccgtcagg gccgcgccgg acgagatgcc
      361 gaccggaatg ccttcgaccg aggcgcaccg tcgggccatg gcgatggcgt cgtcgttgcc
      421 gaccttgacg atgtcgtcga tcacgccgcg gtcgaggatg cccggcacga agcccgcgcc
      481 gatgccctgg atcttgtgcg cgccgggctg accgcccgac agcaccggcg aagcctctgg
      541 ctcgaccgcg accatcttga tcgacggctt gcgcgccttg agcacctggc cgacgccggt
      601 gatggtgccg cccgtgccga cccccgagac gacggcgtcg accgaaccgg ccgtgtcgtt
      661 ccagatctcc tcggccgtcg agacgcggtg gatcagcggg ttggcctcgt tctcgaactg
      721 ctgcggcatg accgagccgg ggatctcctc caggatctcc tgggcccggg ccacggcgcc
      781 ccgcatgccc ttctcggccg gcgtcagctc gagccgcgcg ccgaggatca acagcatctt
      841 gcgccgctcc atcgacatgc tctcgggcat caccagggtg atgcgatagc ccttggccgc
      901 ggcgacgaag gccagggcga tgccggtgtt gccgctggtc ggctcgacga tcatgccgcc
      961 gggcttgagg tggccctggg cctccagcca ctcgatcatg gagacgccga tgcggtcctt
     1021 gaccgagttc atcgggttga agaattccag ctttcccagc acctcggctc tgggcttcag
     1081 ctctgcgctg agccggggca ggcggaccag cggcgtgtcg ccgacggtgt cgaggatgga
     1141 gtcgtaaatc ttgccgcggc ccgcgcgctt gaagcgggcg gcgtcgtaac tggtcatcgg
     1201 aagtctccgg cggagggggc gccctttgga gccgaaaact acagggccag gccggggttg
     1261 tcagcgttca ttcggcggcg agggccgggg cgtcgctttc ggccagcagg ggctccagca
     1321 gatgcgccgc gagccagcgc acgaccggaa cggccacgcc gtcgcctatc acgtggaagg
     1381 cggcggtgtc ccccttcgga agccggtagg agtcgggcag gcccatcagt cgtgcgcctt
     1441 cgcggggcgc gagcagacgc gaacggatca ggctcggctc ggcgacgatc acggtctgtc
     1501 gcgaggagcc gccgcgcggg gtccgcaggc agccggccac gccgtccaga cgcacctccg
     1561 cgcgctggac cccgccacgc atgcgccgga aggccgtgcc gacggcccgg ccctgttccc
     1621 ccgcccgcct caggcggccc aggtgttcag gcgt
//
LOCUS       DAORNX010000588         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_52894_length_1641_cov_6.180328, whole genome shotgun sequence.
ACCESSION   DAORNX010000588 DAORNX010000000
VERSION     DAORNX010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52894_length_1641_cov_6.180328"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLO65_16990"
     CDS             complement(<1..379)
                     /locus_tag="PLO65_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29994.1"
                     /translation="MAVFAASAASPEAQADCPSPYDTATCDGICFERTTTITDDTWTC
                     DLSASTADSEANMVTDYDLSVPYEAWGVYYTGSGPPTMNRFCCESPAADYSFVEIIGS
                     DYGDALCFEWATNTYNLKPAAAQP"
     gene            complement(728..>1641)
                     /locus_tag="PLO65_16995"
     CDS             complement(728..>1641)
                     /locus_tag="PLO65_16995"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRD29995.1"
                     /translation="RFGSALNLNLHFHVLALDGLYCEDPDDGSVHWFRAPAPTDQEVS
                     TLIEQIADRAEAFLARRGFGLDDEQEEDPDDALAVIQAAAVAGRSAVRRSRQARRVQK
                     LGGRPYRLPPTCANCDGYGLHAGVVIGANDRRGLEGLARYIARPPLATPRLEELPDGS
                     LRIRFKRPWSDGTEGVELSRIELIERLAALVPPPHANTVFYHGVLAARSGLRPRVQPR
                     ATPRSQPGGSTACRLARKPAATSRWVPWARLLWRAFEVDGWACPCCDQRMRLWAVVVA
                     PATLGVMESLARSARGPPADGSKTLDA"
BASE COUNT          265 a          541 c          573 g          262 t
ORIGIN      
        1 gcggctgagc agccgccggc ttcaggttgt aggtgttggt ggcccactcg aagcacaatg
       61 cgtcaccgta gtcggagccg atgatctcca cgaagctata gtccgccgct ggcgactcgc
      121 agcagaagcg gttcatggtg ggcgggccgc tgcccgtgta gtagacgccc caggcctcgt
      181 aggggaccga gaggtcgtag tccgtgacca tgttcgcttc cgagtccgcc gtggaggcgc
      241 tcaggtcgca ggtccaggtg tcgtcggtga tcgttgtggt tcgctcgaag cagatgccgt
      301 cgcaggtcgc ggtgtcgtac ggcgaggggc aatccgcctg cgcctcaggc gaagccgcgc
      361 tcgcggcgaa gaccgccatc agggccaggg cacgcggggg ggggaaattc cagagatcgg
      421 gtactccgac gggttgaggg gactgctcgt tcgcgcgagg accacgcatg aggagtctct
      481 cccacacctt ttcgaaagtc aacaggtctc cgaagacttt acaattgaat gcccgttagg
      541 gataggaaac atcaagcctc tgctggcgcc acccctgggc gggtcggtcc ctccctggcg
      601 ccctggatgc ccgcgacttg gcgctctgga gtgggtatcg ccgccgtagc ggggcccctg
      661 gatgcccgta ggaaggtgct ctcctgtcgc caggcgcagc tccgcccgcc cgtcgctgtc
      721 cctcgcttca ggcgtcgagc gtcttcgaac catcggccgg tggcccgcgc gcggacctcg
      781 ccaacgactc catcactcca agcgtcgccg gggcaacgac gacggcccac agccgcattc
      841 tctggtcgca gcacggacaa gcccagccgt cgacttcgaa ggcccgccag agcagccggg
      901 cccatggcac ccaacgcgag gtcgcggccg gcttcctggc gaggcggcaa gcggtcgatc
      961 cgccaggctg tgaacgcggt gtcgctcgcg gctgcaccct gggccgcagc cccgaccggg
     1021 ccgcgagcac cccatggtaa aatacggtgt tggcgtgtgg aggaggaacc agggcggcca
     1081 ggcgttcgat cagctcgatc cgggacagct cgacgccttc ggtcccgtcc gaccagggcc
     1141 gtttgaatcg aatgcgcagg ctaccatcgg ggagctcctc gagccgaggc gtggcgaggg
     1201 gcggtcgcgc gatgtaccgg gcgaggccct ccaagcccct gcgatcgttg gcgccgatga
     1261 ccaccccagc atgcaggccg tagccatcgc agttggcgca cgttggcggc agccggtagg
     1321 gccggccgcc gagcttctgg acgcgccggg cttgccggga gcgccgcacg gcgctgcgtc
     1381 cggcgaccgc cgctgcctgg atcaccgcca aggcgtcgtc gggatcttct tcctgctcgt
     1441 cgtcaagccc gaagcctcgg cgcgcgagga aggcttcggc gcggtcggcg atctgctcga
     1501 tcagggtcga cacctcctga tcggtcggag caggcgcacg aaaccagtgg accgagccgt
     1561 cgtcggggtc ttcgcagtag agcccgtcaa gggccagaac atggaagtgc aggttcaaat
     1621 tcagagcgga gccgaagcgt t
//
LOCUS       DAORNX010000589         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_52930_length_1640_cov_4.336909, whole genome shotgun sequence.
ACCESSION   DAORNX010000589 DAORNX010000000
VERSION     DAORNX010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52930_length_1640_cov_4.336909"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLO65_17000"
     CDS             complement(<1..469)
                     /locus_tag="PLO65_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRD29996.1"
                     /translation="MTFFQRLITETAQGQQTLAAVPQIQDGLAGAISRETYIAYLTEA
                     YHHVKHTVPLMQAARAGLDDAHDRFRHALDEYIAEETGHEAWILNDIRNCGGDPEAVR
                     DGTPRAATRAMVDYAYDYIRRVNPMGFFGMVLVLEGTSVRLATQGAEAVARTLG"
     gene            complement(466..>736)
                     /locus_tag="PLO65_17005"
     CDS             complement(466..>736)
                     /locus_tag="PLO65_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD29997.1"
                     /translation="VDRQFGEAQAELGALIVPLTPDLDEATLARAVERANASLPPYAR
                     IARWTVRPPLDPEAGELTGNGRPRRAAILANHRPFIDAIEQETRL"
     assembly_gap    737..746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<747..>1640)
                     /locus_tag="PLO65_17010"
     CDS             complement(<747..>1640)
                     /locus_tag="PLO65_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD29998.1"
                     /translation="GEDDGWIEAAGERLGVALLATAPTALPPGTAKITYTSGSTGQPK
                     GVCLSQAQMEATAASLVEVIGADYAGRHLPLLPLSILLENVAGLYTTLIAGGRYHVLP
                     AASLGLDNPFRPDLAKLAATIAGEKATSLILVPELLRALLMVMSFAGARFPGLSLVAV
                     GGARVSPRLLDQAASLGLPVYEGYGLSECASVVALNTPDAHRAGTVGKPLPHLQVTVA
                     ADGEILVGPRPFLGYAGGALNAGVVATGDIGGQDADGFLSIAGRRGNTIINAYGRNIA
                     PEWVESELLAQPEIRQAIVFG"
BASE COUNT          220 a          554 c          620 g          236 t
ORIGIN      
        1 ggcccagggt cctggccacc gcctcggcgc cctgggtcgc cagccgcacg ctggtgccct
       61 cgagcaccag caccatgccg aagaagccca tcggattgac ccgccggatg tagtcatagg
      121 cgtagtcgac catcgcccgg gtcgcggcgc gcggcgtccc gtcgcgcacc gcctcgggat
      181 cgccgccgca attgcggatg tcgttgagga tccatgcctc gtgcccggtc tcctcggcga
      241 tgtattcgtc gagggcgtgg cggaagcggt cgtgggcgtc gtccagcccg gccctggccg
      301 cctgcatcag cggcacggtg tgcttcacat ggtgataggc ctcggtcaga taggcgatgt
      361 aggtctcgcg cgagatcgcc ccggccagac cgtcctggat ctgcggcacg gcggccagcg
      421 tctgctggcc ctgggcggtc tcggtgatca gtcgctggaa gaaggtcaca gccgggtctc
      481 ctgttcgatg gcgtcgatga aagggcgatg gttggccagg atggccgccc ggcgcggccg
      541 gccgttgccg gtcagctcgc cggcctcggg gtcgaggggc ggacggacgg tccagcgcgc
      601 gatgcgggcg taggggggca ggctggcgtt ggcccgctcg accgcgcggg cgagggttgc
      661 ttcgtcgagg tcgggcgtca gcggcacgat cagggcgccg agctcggcct gggcctcgcc
      721 gaactgacga tccacannnn nnnnnncgcc gaaaacgatc gcctggcgga tttcgggctg
      781 ggccagcagc tcgctctcga cccactcggg cgcgatgttg cggccgtagg cgttgatgat
      841 ggtgttgccg cgccggccgg cgatcgacag gaagccgtcg gcgtcctggc cgccgatgtc
      901 gccggtggcg acgacgcccg cgttcagcgc cccgcccgcg tagccgagga acggccgggg
      961 gccgacgagg atttcgccgt ccgccgccac ggtgacctgc aggtgtggca acggcttgcc
     1021 gaccgtgccc gcgcgatggg cgtcgggggt gttcagggcg accaccgagg cgcattcgct
     1081 caggccgtag ccctcgtaga ccggcaggcc cagcgaagcg gcctgatcga gcaggcgcgg
     1141 cgacaccctg gcgccgccca cggccacgag gctgagcccg gggaatcgcg cgccggcgaa
     1201 cgacatgacc atcagcagcg cgcgcagcag ttcgggcacg aggatcaggc tggtggcctt
     1261 ctcgccagcg atcgtggccg cgagtttggc caggtccggc cggaacgggt tgtcgaggcc
     1321 gagactggcc gccggcagga cgtgatagcg cccgcccgcg atcagggtcg tgtagagccc
     1381 ggcgacgttc tccagcagga tcgacagcgg cagcagcggc aggtgccggc cggcgtagtc
     1441 ggccccgatc acctcgacca gcgaggcggc ggtcgcctcc atctgcgcct gcgacaggca
     1501 gacacccttg ggctggccgg tcgagccgga agtgtaggtg atcttggccg tgcccggggg
     1561 cagggcggtc ggcgcggtgg ccagcagggc gacgcccagc cgctcgccgg ccgcttcgat
     1621 ccagccatcg tcctcgccgg
//
LOCUS       DAORNX010000590         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_53193_length_1632_cov_4.824984, whole genome shotgun sequence.
ACCESSION   DAORNX010000590 DAORNX010000000
VERSION     DAORNX010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53193_length_1632_cov_4.824984"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLO65_17015"
     CDS             <1..790
                     /locus_tag="PLO65_17015"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRD29999.1"
                     /translation="GTAAARGRLVIDSGAARVEAVQVLWRMTPTLDSTMHFGGRLAFS
                     PDGFLFITTGERSILPGRAQAQRLDGTLGKVVRINPDGSIPKDNPFAAVTEANRSIWS
                     YGHRNLQGAAINPWTGRLWTVEHGARGGDELNIPQGGKDYGWPTITYGEEYSGQPIGE
                     GITAKAGLEQPVYYWDPVIAPSGMAFYDAALFPAWKGSLFIGGLGSKKLVRLTLDGDR
                     VTGEEWLLQDLKQRIRDVRVGPDGAVYVATDDADGKVLKLVPSK"
     gene            complement(935..1492)
                     /locus_tag="PLO65_17020"
                     /pseudo
     CDS             complement(935..1492)
                     /locus_tag="PLO65_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          252 a          552 c          596 g          232 t
ORIGIN      
        1 cgggacggcc gccgcgcgcg gcaggctggt catcgacagc ggcgcggcgc gggtcgaggc
       61 cgtgcaggtc ctctggcgca tgacgccgac gctcgactcg accatgcact tcggcggccg
      121 gctggccttc tcgcccgacg gcttcctgtt catcaccacc ggcgagcggt cgatcctgcc
      181 gggccgggcc caggcgcagc ggctggacgg gacgctcggc aaggtcgtgc gcatcaatcc
      241 cgacggctcg atccccaagg acaatccgtt cgccgccgtg accgaggcga accggtcgat
      301 ctggtcctat ggccatcgca acctgcaggg cgccgcgatc aacccctgga ccgggcggct
      361 gtggaccgtc gagcacggcg cgcgcggcgg cgacgagctg aacatcccgc agggcggcaa
      421 ggactacggc tggccgacca tcacctacgg cgaggagtat tcgggccagc cgatcggcga
      481 ggggatcacg gcgaaggcgg gcttggagca gccggtctac tactgggatc cggtgatcgc
      541 gccgtccggc atggccttct acgacgccgc cctgttcccg gcctggaagg gcagcctgtt
      601 catcggcggg ctggggagca agaagctggt gcggctgacg ctggacggcg accgcgtgac
      661 cggtgaggaa tggctgctgc aggacctgaa acagcgcatc cgcgacgtgc gcgtgggccc
      721 ggacggggcg gtctatgtcg cgacggacga tgccgacggg aaggtgctga agctggttcc
      781 ctcgaagtaa tcctcccctc gtagagggga gggggaccat gcggagcatg gtggaggggc
      841 cagcgccgga ctgaccggaa agtccggaat tcaggacggg gccgccaaca accacgccgc
      901 ccctccaccg ccctccgggc ggtccccctc cccgctacgc ggagaggaga ttcgcaacct
      961 cgtcacccaa tgccagcgag ctcgtcaggc ccgggctttc gatgccgaac atcgccacca
     1021 ggcccgggat gccgtgcata tcctcgccgt cgaggcggaa gtccggttgc ggctcgtcgg
     1081 ggccgtggat cttggggcgc aggccggcgt agtccgggga caggtggtcc tcgggcacgg
     1141 ccggccagaa cttgcggata taggcggcga aggcggcggc ccgggccggg tctaccgaat
     1201 agtcctcggt ctcgacgaac tcgaggtcgg ggccgaacac cgcctggccg ccgagatcct
     1261 tgcggtagtg ggtgccgagc gcgccgtgga tcggcggcgg gtagatcagg tgttcgaacg
     1321 gcgccttgcc cgccaggcgg aaatagaccc ccttgccgaa gtgacgggcc gggatcctgg
     1381 cggccgggaa gccgtcgatc atcgccgccg ccgcctgggc gccgaggccg gcggccgtga
     1441 ccagccggcg cacggtcagg gtcgtcggcg cctcgccgcc ggcgcgcacg gttttccagc
     1501 gccgcgtgcg catgaactcg ttcctcaacc gctggctcga atacctcgcc ggccagggcc
     1561 actcgatcgg gacgctcgaa ccggcgccat cgcccagtgc tgttcagtgt gcaatgggta
     1621 tttgaccaca cg
//
LOCUS       DAORNX010000591         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_53451_length_1625_cov_5.675159, whole genome shotgun sequence.
ACCESSION   DAORNX010000591 DAORNX010000000
VERSION     DAORNX010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53451_length_1625_cov_5.675159"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLO65_17025"
     CDS             <1..967
                     /locus_tag="PLO65_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRD30000.1"
                     /translation="ERKAAWDGYLKTGSSSLLMLEAKGLSEGVPTAGGYVAPPETERL
                     IERRLQMSSPMRDIATVRTIGAGVFRKPVSTAGIESGWVAETDARPETDPPVLALLEF
                     PAADLYASPAATQALLDDAFVNLDEWLAAECEDAFAGQETQAFVNGDGTNKPKGFLSY
                     SKVANAGHAWGDIGYVASGAAGDFDATDPVDRIIDLIYAPKAQYRAHGRFVLNRRTAA
                     KIRKFKDADGNYIWSPATAPGAPATLLGYPVTEIETMPDVAADSFALAFGDFAKGYLI
                     VDRAGVRVLRDPYSAKPYVLFYTTKRVGGGVQNFDAIKLMKFAAG"
     gene            1141..1407
                     /locus_tag="PLO65_17030"
     CDS             1141..1407
                     /locus_tag="PLO65_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HRD30001.1"
                     /translation="MPPITLAEAKAFLRVTDDAEDGLVQLLIDAAIERVEAATGLTLT
                     DISAAPLRLALLMLVAHGFEHRERAEAPWALVEPWLASYRTVRL"
     gene            1404..>1625
                     /locus_tag="PLO65_17035"
     CDS             1404..>1625
                     /locus_tag="PLO65_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30002.1"
                     /translation="MTLGALRTLAVLESASEAETVFGGRTRSWVETAALWIDLRPTGH
                     RTAGDRTVGRGLVETAEAVARSHAAAARGQ"
BASE COUNT          239 a          592 c          574 g          220 t
ORIGIN      
        1 cgagcgcaag gcggcctggg acggctatct gaagaccggt tcgtcctcgc tgctgatgct
       61 cgaggccaag gggctcagcg agggcgtgcc cacggccggc ggctacgtcg cgccgcccga
      121 gaccgagcgg ctgatcgagc ggcggctgca gatgagctcg ccgatgcgcg acatcgccac
      181 cgtgcggacc atcggggcgg gggtgttcag gaagcccgtc agcaccgccg gcatcgagag
      241 cggctgggtg gccgagaccg acgcgcggcc ggagaccgat ccgcccgtgc tggcgctgct
      301 cgagttcccg gcggccgacc tctacgccag ccccgcggcg acccaggccc tgctcgacga
      361 cgccttcgtc aatctcgacg aatggctggc cgccgagtgc gaggacgcct tcgccggcca
      421 ggagacgcag gccttcgtca atggcgacgg gaccaacaag cccaagggct tcctgagcta
      481 cagcaaggtc gccaacgccg ggcatgcctg gggcgacata ggctatgtcg cgtccggcgc
      541 ggccggcgac ttcgacgcca ccgatccggt cgaccggatc atcgacctga tctatgcgcc
      601 caaggcccag taccgggcgc acgggcggtt cgtgctcaac cggcgcaccg cggcgaagat
      661 ccgcaagttc aaggacgccg acggcaacta catctggtcg ccggccacgg cgccgggcgc
      721 ccccgcgacc ctgctcggct atccggtgac cgagatcgag accatgccgg acgtggcggc
      781 cgacagcttc gccctggcct tcggcgactt cgccaagggc tatctgatcg tcgaccgggc
      841 cggggtgcgg gtgctgcgcg acccctattc ggccaagccc tacgtgttgt tctacacgac
      901 caagcgcgtc ggcggcgggg tgcagaactt cgacgccatc aagctgatga agttcgcggc
      961 cggctagccg aacgaccctc tccccccgag ggagagggtg gccgcgcaag cggccgggtg
     1021 aggggccgcg ccgtcccctc cgcgtcccct caccacgccg ccgccgacat tcacgcggcc
     1081 cctcatccga cctgctccgc aggccacctt ctccctcgag gggagaagga ggaggttcac
     1141 atgcccccca tcaccctcgc cgaggcgaag gcgtttctgc gcgtcaccga cgacgcggag
     1201 gacggccttg tccagttgct gatcgacgcc gcgatcgagc gcgtcgaggc ggcgaccggc
     1261 ctgacgctca ccgatatttc cgcggcgccg ctgcggctgg ccctgctgat gctggtcgcc
     1321 cacggcttcg agcatcgcga acgggccgag gcgccgtggg cgctggtcga gccgtggctg
     1381 gcgtcctacc ggacggtgcg gctgtgaccc tcggcgccct gcgaaccctg gccgtgctcg
     1441 aaagcgcttc ggaggccgag accgtgttcg gcggccggac ccggtcatgg gtcgagacgg
     1501 ccgccctgtg gatcgacctg cggccgaccg ggcaccgcac cgccggagac cggaccgtgg
     1561 ggcgggggct ggtggagacg gccgaggccg tcgcccggtc ccatgccgcc gccgcccgcg
     1621 gccag
//
LOCUS       DAORNX010000592         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_53526_length_1623_cov_4.044005, whole genome shotgun sequence.
ACCESSION   DAORNX010000592 DAORNX010000000
VERSION     DAORNX010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53526_length_1623_cov_4.044005"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1255
                     /locus_tag="PLO65_17040"
     CDS             <1..>1255
                     /locus_tag="PLO65_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRD30003.1"
                     /translation="VNRLAAGQGAGVQVGAAFAGLALGWAMIRFLAAAAPQARDAIVT
                     PVAQAAQARAASETFAHALSLSMDFHQSKRTGSLSRVIDRGARSMDFLLRALVFNLAP
                     TLIELFIAAGVLAGKYDWRFAAVAVATVIVYVVLTFAVSDWRIAHRRALNEADQDAAG
                     RAVDALINYETVKTFGAEARAVANYEVALGRYSAAQVRATNSLTLLNSAQSGIMSLGL
                     ALMAVMAGLEAAEGRMGPGDVTAAVLIMINLYGPLNILGFAYREIRQSFIDMEAMLDL
                     RRQSAEVAEAADARDLPPAPAGQGGEVVFEAVSFRHGARSEGISEVSFRAAPGATVAL
                     VGPSGAGKTTLVRLALRLIGCLIGAVAALLVFDAAFGFVALLGVIALAGMIMRNTIIL
                     VDQVRQDLADGRSPHDAIVESTVRRA"
     assembly_gap    1256..1415
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          198 a          518 c          512 g          235 t
ORIGIN      
        1 gtcaaccgcc tggcggccgg gcagggggcc ggcgtccagg tcggggcggc cttcgccggc
       61 ctggcgctcg gttgggcgat gatccgcttc ctcgccgccg ccgcccccca ggcgcgcgac
      121 gccatcgtca cccccgtcgc ccaggcggcc caggccaggg cggcgtcgga gaccttcgcc
      181 cacgccctgt cgctgtcgat ggacttccac cagagcaagc gcaccggttc cctgtcgcgg
      241 gtcatcgacc gtggcgcccg ctcgatggat ttcctgctgc gggcgctggt gttcaatctg
      301 gccccgactc tgatcgagct gttcatcgcc gcgggggtgc tggcgggcaa gtacgactgg
      361 cgcttcgcgg ccgtcgccgt cgcgaccgtg atcgtctatg tcgtcctgac cttcgccgtg
      421 tccgactggc ggatcgccca ccgccgggcg ctcaacgagg ccgaccagga cgcggccggc
      481 cgggcggtcg acgccctgat caactacgaa acggtcaaga ccttcggggc cgaggcccgg
      541 gcggtggcca actacgaggt cgcccttggc cgctacagcg ccgcccaggt ccgggcgacc
      601 aattccctga ccctgctgaa ctcggcccag tcggggatca tgagcctggg cctggccctg
      661 atggccgtga tggccgggct ggaggcggcc gagggccgca tgggccctgg cgacgtcacc
      721 gccgctgtgc tgatcatgat caacctctat ggcccgttga acatcctggg cttcgcctac
      781 cgggaaatcc ggcagtcatt catcgacatg gaggcgatgc tcgacctgcg ccggcaatcg
      841 gccgaagtgg ccgaggccgc cgacgcccgc gatctgccgc cggccccggc cgggcagggc
      901 ggggaggtgg tgttcgaggc cgtctccttc cgccacggcg cccggtcgga gggtatcagc
      961 gaggtcagct tccgggccgc gcctggcgcc accgtggcgc tggtcggccc gtccggcgct
     1021 ggcaagacca ccctggtgcg gctggccctg cggctgatcg gttgcctgat cggcgcggtc
     1081 gccgccctgc tggtcttcga tgccgccttc ggcttcgtgg cgctgctggg cgtgatcgcg
     1141 ctcgccggca tgatcatgcg caacaccatc atcctggtcg accaggtgcg ccaggacctc
     1201 gccgacggcc gcagcccgca cgacgccatc gtcgaatcca ccgtccgccg ggcgcnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggc gctgcttgcg ttcgaccgct
     1441 tcctgcatgc cctgccggcc ggcgttcagc gcgccggata ggcgcgcggg tagatcacct
     1501 gatcgggcgg accgggtggg ctcggggggg ccttgcggcc gcaggcggac aggatcaagg
     1561 caaggacaag caagggaagg atcttctttt ttgtgaacaa aaaagaagca aaaaaaactt
     1621 cat
//
LOCUS       DAORNX010000593         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_53715_length_1618_cov_5.833013, whole genome shotgun sequence.
ACCESSION   DAORNX010000593 DAORNX010000000
VERSION     DAORNX010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53715_length_1618_cov_5.833013"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLO65_17045"
     CDS             <1..467
                     /locus_tag="PLO65_17045"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRD30004.1"
                     /translation="GGWRMAARGLAQNPVLWGCAVGAAFNLLHVPKLPVVMSTLDLLS
                     AAALTMGLVVAGAGLNFGYVHSRRWLIAAVSAVKLLVLPVLMWGFCRLAGGDGTAQGI
                     ALLAGSAPGAAASYVLARQMGGDAPLMAGIVAFTTAASLVTIPLLLVVFHVG"
     gene            587..1564
                     /locus_tag="PLO65_17050"
     CDS             587..1564
                     /locus_tag="PLO65_17050"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRD30005.1"
                     /translation="MTAFAPGIDEVRARTFPFPRRHFLSALDVNVVEAQQLLDLADGF
                     VALNRQTTKKLDLLKGRTLMNLFFENSTRTQASFELAGKRLGADVMNMSVKTSSVAKG
                     ETLIDTAVTLNAMQPDLLVVRHASSGAAALLSQKVGCSVINAGDGQHEHPTQALLDAL
                     SMRRAFGRIAGLTVAICGDVMHSRVARSNVNLLNILGANVRLVGPPTLMPAGADRWGA
                     AVFHDLEKGIAGADVVMMLRLQLERMQGALVPSTREYFRFWGLDREKLKAASDGVRVM
                     HPGPMNRGVEIDSDVADDPGVSLIQDQVEMGVAARMAVLASLAARLDNH"
BASE COUNT          230 a          548 c          573 g          267 t
ORIGIN      
        1 gcggcggctg gcgcatggcc gcgcgcgggc tggcgcagaa cccggtgctg tggggctgcg
       61 cggtgggggc ggcgttcaac ctcctgcatg ttccgaagct gccggtggtc atgagcaccc
      121 tggacctgct gtcggccgcc gccctgacca tggggctggt ggtcgcaggg gcgggactca
      181 acttcggcta cgtccacagc cgccgctggc tgatcgcggc ggtcagcgcg gtcaagctgc
      241 tggtcctgcc ggtgctgatg tggggcttct gccggctggc cggcggcgac ggcacggccc
      301 agggcatcgc cctgctggcc ggctcggcgc ccggcgcggc ggcctcctac gttctggcgc
      361 ggcagatggg cggagacgca ccgctgatgg ccggcatcgt cgcattcacc acggcggcga
      421 gcctggtgac catcccgctg ctgctggtgg tgtttcatgt ggggtgacgg aggggacatg
      481 taccgcaggt acgtgtcccc caatcgagtg tcccccaatc gagacgcagg ggtttagggg
      541 acacgtacct tgcaggtaca tgtcccctcg cctatgacca ccgcttatga cggctttcgc
      601 cccgggtatc gatgaggtcc gcgcacggac cttccccttc ccccgccgtc atttcctgtc
      661 cgcgctggat gtcaacgtcg tcgaggcgca gcagctgctc gacctggccg acgggttcgt
      721 ggcgctgaac cggcagacga ccaagaagct cgacctgctc aagggtcgca cgttgatgaa
      781 cctgttcttc gagaactcga cgcgcaccca ggccagtttc gagctggccg gcaagcggct
      841 gggcgccgac gtgatgaaca tgagcgtgaa gacctcgtca gtggccaagg gcgagaccct
      901 gatcgacacg gcggtgacgc tgaacgccat gcagccggac ctgctggtcg tgcggcacgc
      961 ctcgtccggc gcggcggccc tgctgtcgca gaaggtcggc tgcagcgtga tcaacgccgg
     1021 cgacggccag cacgagcacc cgacccaggc cctgctcgac gccctgtcga tgcgccgcgc
     1081 gttcggccgg atcgccggcc tgacggtggc catctgcggc gatgtgatgc acagccgggt
     1141 ggcgcgctcg aacgtcaatc ttctcaatat cctgggggcg aatgttcgcc tcgtcgggcc
     1201 gcccaccctg atgcccgccg gggccgaccg ctggggcgcg gccgtgttcc acgacctcga
     1261 aaagggcatc gccggggccg acgtggtgat gatgctgcgg ctgcagctcg agcggatgca
     1321 gggcgcgctg gtcccctcga cccgcgaata tttccgtttc tggggcctcg accgcgagaa
     1381 gctcaaggcc gcctcggacg gggtgcgcgt catgcatccc ggaccgatga accgcggcgt
     1441 ggagatcgac tccgacgtgg ccgacgatcc gggggtcagc ctgatccagg accaggtgga
     1501 gatgggcgtg gccgcccgca tggccgtact ggcctcgctc gccgcccggc tggacaatca
     1561 ctgatggccc gcgcccgtcc gatcgccttc cagaacgccc ggctggtcga cccggaga
//
LOCUS       DAORNX010000594         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_53882_length_1614_cov_7.575369, whole genome shotgun sequence.
ACCESSION   DAORNX010000594 DAORNX010000000
VERSION     DAORNX010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53882_length_1614_cov_7.575369"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLO65_17055"
     CDS             complement(<1..363)
                     /locus_tag="PLO65_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HRD30006.1"
                     /translation="MTQMELIVAATAGSVCLAICAVLWALVQRRTMDRRLRAMQDRLE
                     AVESRAAAAQASAEAFDSSLLVIDDGRALLASGEENLEACAAAFALTAADPQGMVNAI
                     MRSDPDHAARLRALFERRE"
     gene            458..862
                     /locus_tag="PLO65_17060"
     CDS             458..862
                     /locus_tag="PLO65_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRD30007.1"
                     /translation="MTYPSAATAPSLDDFAALAEAAFEALPALFRQMAGETIIRVADF
                     AEDDVLRDLDIEDPFELTGLYQGVDLSRRSVLDPLPHPSMVFLYRRPILDEWAERGDV
                     GLGDLIAHVLVHEIGHHFGLSDADIDAIEDKA"
     gene            885..1316
                     /locus_tag="PLO65_17065"
     CDS             885..1316
                     /locus_tag="PLO65_17065"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRD30008.1"
                     /translation="MNAPDFDYEAVRRDMPEARVMRGEAYAAKGWVALVEVSPAGVLA
                     HVTGERGIAYVVEVAARDASDARCTCPDFEESGLRCKHVVATVFTVLDTDDAGLAAAA
                     GRLARLADQLDMEGRDDALLLVDRARRDPALLRELEGDPVI"
BASE COUNT          244 a          571 c          545 g          254 t
ORIGIN      
        1 ctcgcgccgc tcgaacaggg ctctcagccg cgccgcgtga tcagggtcgg agcgcatgat
       61 ggcgttgacc atgccctgcg ggtcggccgc ggtgagggcg aaggcggcgg cgcaggcctc
      121 gaggttctcc tcgcccgagg ccagcagcgc gcggccatca tcgatcacca gcaggctgct
      181 gtcgaaggcc tcggccgagg cctgggcggc ggcggcgcgg ctttccacgg cctccagccg
      241 atcctgcatc gcgcgcaggc gccggtccat cgtccgccgc tgcaccagcg cccacagcac
      301 cgcgcagatg gcgaggcaga cggagccggc cgtggcggcg acgatcaatt ccatctgcgt
      361 catgcaagac ccgcccgaat catggcccgg acaagatagg cgaggccggc ccggcccgcg
      421 actggcgaag cgaccggcgc ggcgccatct tttcccgatg acctatccat cagccgcaac
      481 ggctccgtcc ctcgacgatt tcgccgccct cgccgaggcc gccttcgagg ccctgccggc
      541 gctgtttcgc cagatggccg gcgagacgat catccgggtc gctgacttcg ccgaggatga
      601 cgtcctgcgg gacctggaca tcgaggaccc gttcgagctg accgggctct accagggcgt
      661 agacctgtcg cgccgctcgg tgctcgatcc cctgccgcac ccgtcgatgg tgttcctgta
      721 ccggcggccg atcctcgacg aatgggccga gcgcggcgac gtgggcctgg gcgacctgat
      781 cgcccatgtg ctggtgcacg agatcggcca tcatttcggc ctgtcggacg ccgacatcga
      841 cgccatcgaa gacaaagcgt gaacgaatcg gctaggctgg cccgatgaac gcgcccgatt
      901 tcgactatga ggccgtccgc cgcgacatgc ccgaggcgcg ggtgatgcgc ggcgaggcct
      961 atgcggccaa gggctgggtc gcgctggttg aggtctcgcc cgccggcgtg ctggcccatg
     1021 tgaccggcga acgcggcatc gcctatgtcg tcgaggtcgc ggccagggac gcgtccgacg
     1081 cccgctgcac ctgtcccgat ttcgaggaaa gcggcctgcg ctgcaagcat gtggtggcga
     1141 ccgtgttcac cgtgctcgac accgacgacg ccggcctggc ggccgccgcc ggacgcctgg
     1201 cgcggctggc cgatcagctc gacatggaag gtcgtgacga cgccctgttg ctggtcgacc
     1261 gggcccggcg cgacccggcc ctgctgcgcg agcttgaggg cgatccggtc atctgacgcg
     1321 gcctccctgc tcctcacggc atgggcggcg gaagggtgtt cgacccctcg cattccgccg
     1381 gcgtcgcttc ccccgctcgc cacgtcgcgc cgggccagcg ttaacttccc gtaagaactt
     1441 gacgcccccg tcattttcta attgacccgc attcgcatta agtggacagt gttcacattg
     1501 gacgaggtcc gactgcaacg ggcccgcatt cccttgatct ggccggagga ccgttgccat
     1561 gagcgacaag aacatcacca aggacgccat gtacgacgcg gtggcgccgg acga
//
LOCUS       DAORNX010000595         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_54505_length_1598_cov_6.261828, whole genome shotgun sequence.
ACCESSION   DAORNX010000595 DAORNX010000000
VERSION     DAORNX010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54505_length_1598_cov_6.261828"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLO65_17070"
     CDS             <1..1183
                     /locus_tag="PLO65_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD30009.1"
                     /translation="SGTVETQRTLVIQDGKVLRIEAGYTAAAGARVIDLHDSFVLPGL
                     IDSHVHLTSEQGPNAQLEEFTKTRSDLALDGAWNGLKTLKAGFTTVADLGAPNESIFA
                     LRDAIRTGRIAGPRIIAAGSPISVHGGHGDPGNGMPEALVPVYRGPAVCSGADDCRRA
                     VREQVRAGADIIKITATGGVLSQTAAGLAKQFSDDELRAIVETAHSMGRKVTAHAHGA
                     DGINSFLRAGGDSIEHGTYLDAESIKLFRSTGAWLIPTLMAGDYVAREAARPNTFFSP
                     AQKAKALEAGPKMLDMARRAHDGGVRIAFGTDTGVSAHGDNAGEFALMVKAGFTPLDA
                     IRAATIWAAEHFGLTDQIGALLPGKQADLIAVRGDPLTDVTVLTKVSFVMKGGVVYKS
                     E"
     gene            complement(1206..>1598)
                     /locus_tag="PLO65_17075"
     CDS             complement(1206..>1598)
                     /locus_tag="PLO65_17075"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRD30010.1"
                     /translation="LFAGGPVLVARLEPDAPPPPGPQVGFAGIGKPWKVERSLVAAGC
                     DLKDFVRFPDHHAYGAGDLALLDEVAARHGAGLVTTEKDWVRLPPEWRARATVWPVRA
                     RFEDEAAVLALLGTCTSAPVPEAAGDTR"
BASE COUNT          254 a          594 c          540 g          210 t
ORIGIN      
        1 cagcggcacg gtcgagaccc agcggaccct ggttatccag gatggcaagg tgctgcggat
       61 cgaagccggc tataccgccg ccgccggcgc ccgcgtcatc gacctgcatg acagcttcgt
      121 cctgcccggc ctgatcgaca gccatgtgca cctgaccagc gaacagggcc ccaacgccca
      181 gctcgaggaa ttcaccaaga cccggtcgga cctggccctc gacggcgcct ggaacggcct
      241 caagacgctg aaggccggct tcaccacggt ggccgacctg ggggcgccca acgaatcgat
      301 cttcgccctg cgggatgcga tcaggaccgg ccgcatcgcc ggtccgcgca tcattgccgc
      361 cggctccccc atctcggtgc acggcggcca cggcgacccg ggcaacggca tgcccgaggc
      421 cctggtcccg gtctatcggg gaccggcggt ctgctcgggc gccgacgact gccgccgggc
      481 ggtgcgtgaa caggtccggg ccggcgccga catcatcaag atcacggcca ccggcggggt
      541 gctgtcccag accgccgcgg gcctcgccaa gcagttctcg gatgacgaac tgcgggcgat
      601 cgtcgagacc gcccattcga tgggccgcaa ggtcaccgcc cacgcccacg gcgccgacgg
      661 catcaactcc ttcctccgcg ccggcggcga ttccatcgag cacggcacct atctcgacgc
      721 cgagtcgatc aagctgttcc ggtcgacggg ggcctggctg atcccgaccc tgatggccgg
      781 cgactacgtg gcgcgcgagg cggcgaggcc caacaccttc ttcagcccgg cccagaaggc
      841 caaggcgctc gaggccggcc cgaagatgct cgacatggcc cgccgcgccc atgacggcgg
      901 ggtcaggatc gccttcggca ccgacaccgg cgtctcggcc catggcgaca atgccggaga
      961 gttcgccctg atggtcaagg cgggcttcac cccgctggac gccatccgcg cggcgactat
     1021 ctgggcggcc gaacacttcg gcctcaccga ccagattggc gcgctgctcc cgggcaagca
     1081 ggccgacctg atcgccgtgc gcggcgaccc gctgaccgac gtgacggtgc tgacgaaggt
     1141 cagtttcgtg atgaagggcg gggtggtcta caagagcgaa tagtcttccg gagggggcat
     1201 gcccttcagc gcgtgtcccc cgccgcttcg ggaacaggcg ccgacgtgca tgttcccaac
     1261 agagcgagca ccgccgcctc gtcctcgaac cgcgcccgca cgggccagac ggtcgccctc
     1321 gcgcgccatt cgggcggcag gcggacccag tccttctcgg tcgtgaccag gccggcgccg
     1381 tgccgcgcgg cgacctcgtc cagcagcgcc aggtcaccgg cgccataggc gtgatggtcg
     1441 gggaagcgca cgaagtcctt caggtcgcag ccggccgcga ccagtgagcg ctcgaccttc
     1501 cacggcttgc cgatacccgc gaagccgacc tggggcccgg gcggcggcgg cgcgtccggc
     1561 tcgagccggg cgaccagcac cggaccgccg gcgaacag
//
LOCUS       DAORNX010000596         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_55046_length_1585_cov_4.053595, whole genome shotgun sequence.
ACCESSION   DAORNX010000596 DAORNX010000000
VERSION     DAORNX010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55046_length_1585_cov_4.053595"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..693)
                     /locus_tag="PLO65_17080"
     CDS             complement(181..693)
                     /locus_tag="PLO65_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD30011.1"
                     /translation="MSEVAIRRLMPADVEAFRALRIEAMTAEPETFGTDILEERAKTL
                     EASAATLESTALFGGFVDDALVGMAGYSRLKPLREQHKGLVWSMYVRASARGHGLGGR
                     LLRAVIDHARGEVESLQLVVVSTNTAAVALYERHGFRRWGLEPFALKVGEGRYTTDAW
                     YWLPLAGLEA"
     gene            complement(690..>1585)
                     /locus_tag="PLO65_17085"
     CDS             complement(690..>1585)
                     /locus_tag="PLO65_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRD30012.1"
                     /translation="PGQALSWRPVRGQQWPMMTRLLLCLALLLTAAPALAQPVAARPI
                     VLGETFDLPSRAEGETRHITVWLPPSYAAGKAAYPVLYLLDGGEAQDLLPVVGLAALG
                     GLNGVTDEMIVVGIESGPGLRLRDFTTPSEVERKQFPVQGEAARFRRFLAQELLPLVA
                     GRYRTSGETALMGESLAGYFVLDSALRQPDLVTTYIAVSPSIWWDDQSLVRDAATLLK
                     ARPWPPGRKLYLSQANEGALVAGAMSRLLATLRADGPPQLAWTFRAFPQERHLTTYHP
                     SGLEALRWAFPGPEAEPARAP"
BASE COUNT          227 a          542 c          601 g          215 t
ORIGIN      
        1 tcgagaatcc cgcgaatccg gcgctgggcc tgcggatcgc tctgctcgtc cggctcgaag
       61 tgatcgttct ggcggcggaa gctgttggtg cgggttacgg acatgacgtc ctgccttcag
      121 gcggaccggg cgcggtcctg tgagcccgcg aaggttagaa tgtgtcgcgt taacacagcg
      181 ttaggcctcg agaccggcca gcggcagcca gtaccaggcg tcggtggtat agcggccctc
      241 gccgaccttg agggcgaacg gctccaggcc ccagcggcgg aacccgtgac gctcgtagag
      301 ggccacggcc gcggtgttgg tcgagacgac caccagctgg aggctctcga cctcgccgcg
      361 ggcatggtcg atcaccgccc gcagcagccg cccgcccagg ccatggccgc gggccgaggc
      421 ccggacgtac atgctccaga ccaggccctt gtgctgctcg cgcaaaggct tgagccgcga
      481 gtagccggcc atgccgacca gcgcgtcgtc gacgaagccg ccgaacaggg cggtggactc
      541 gagggtcgcg gcgctggctt cgagggtctt ggcgcgctcc tcgaggatgt ccgtgccgaa
      601 ggtctccggc tcggcggtca tggcctcgat ccgcagggcg cggaaggcct cgacgtcggc
      661 gggcatgagc cggcggatgg cgacctcgct cacggcgccc tcgccggttc ggcctccggc
      721 cccgggaagg cccagcgcaa ggcctccagg cccgacggat gataggtcgt caggtggcgt
      781 tcctggggaa aggcgcggaa ggtccaggcg agctgcggcg ggccgtcggc gcgcagcgtc
      841 gccagcaggc ggctcatcgc cccggccacc agcgccccct cgttggcctg cgacaggtag
      901 agcttgcggc cgggcggcca gggccgggcc ttcagcaggg tggcggcgtc gcgcacaagg
      961 ctctggtcgt cccaccagat gctcggcgag acggcgatgt aggtggtcac caggtccggc
     1021 tggcgcaggg cgctgtcgag cacgaagtag ccggccaggc tctcgcccat cagggccgtc
     1081 tcgccgctgg tgcggtagcg cccggcgacc agcggcagca gctcctgcgc caggaagcgg
     1141 cggaagcggg ccgcctcgcc ctggaccgga aactgcttgc gctcgacctc gctgggcgtg
     1201 gtgaagtcgc gcagcctcag gcccgggccg ctctcgatcc cgaccacgat catctcgtcg
     1261 gtgacgccgt tgagcccgcc cagggcggcc aggccgacga ccggcagcag gtcctgcgcc
     1321 tcgccgccgt cgagcaggta gagcaccgga taggcggcct tgccggcggc gtaggacggc
     1381 ggcagccaga cggtgatgtg gcgggtctcg ccctcggcgc gcgagggcag gtcgaaggtc
     1441 tcgcccagca cgatcggcct ggcggcgacc ggttgggcca gggccggcgc ggcggtcagc
     1501 agcagggcga ggcagagcag caggcgagtc atcatcggcc attgttgacc gcgaaccggt
     1561 cgccagctca aggcctggcc gggcg
//
LOCUS       DAORNX010000597         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_55090_length_1584_cov_5.132767, whole genome shotgun sequence.
ACCESSION   DAORNX010000597 DAORNX010000000
VERSION     DAORNX010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55090_length_1584_cov_5.132767"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..299)
                     /locus_tag="PLO65_17090"
                     /pseudo
     CDS             complement(1..299)
                     /locus_tag="PLO65_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            588..1382
                     /locus_tag="PLO65_17095"
     CDS             588..1382
                     /locus_tag="PLO65_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30013.1"
                     /translation="MTVRRTLTAFNTATASENRIHADDVASRFGFTGGLVPGVDVFAY
                     MAHAPVARWGRDWLSQGRIRAKFGKPVYDGDETTVAADEQPDGSLALTVTARGLDCAS
                     GTAWRSDDGAVPAILPEADLPRPEDRPPASPDSLPVGRVLGALKELYFADVGLSHLQN
                     TREDPALFDGGDLCNPAYMLRRANYVLAQSVKLGPWIHLESDARLHGLIRDGEALETR
                     GVVVANEEKNGHRIVTLDFTVSSRERLAMSGRHWAIYEPRQVREKG"
     gene            complement(1379..>1584)
                     /locus_tag="PLO65_17100"
     CDS             complement(1379..>1584)
                     /locus_tag="PLO65_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30014.1"
                     /translation="ATADPRPDPTPAEALARWGTLTGQELAFLCGESATPPTGAVGHD
                     WGDGMVWFSPAEARARGLAAVAA"
BASE COUNT          243 a          547 c          574 g          220 t
ORIGIN      
        1 tccggcggct cgaccgtgac ggacgaaggc cgcacgtaga cttccggccg gtgcagatag
       61 atctgcggcg cggcgatgcg gatcggcggc gcgtcgacgt agacgggcgg accctggata
      121 tcgatgcggc ccgacggcac cacgaccggg gtgccgtaga cgcggacgcc cggcgcgcga
      181 acgcggatgt cggcccaggg cgcggtcggg acgtagaagg tctcgaggct ggtgcagagg
      241 cagtcgatcg gccccggcgg cgggccgtag ccaccgccac tcggcggcgg gggcggcggg
      301 ccgtacggat catgggcgct ggcgaccccg gccgcggcgc tcaacacgag aatgctcaaa
      361 ccggcgataa gaacgtgctt cacttcagct cccccttgtg agccgccgac ctcatgtcgt
      421 gactccgata ccgcctgtca tccgccggag ggagtccggg tgagcgcaca gcggtatgtg
      481 acattaacca agtgttagcc ccaggcgttc ctgcttgcaa ctgccggggg aaggcgattg
      541 tcgttcagac tcctgacgcg ggaatttttc cccttcgtgg gcatttcatg accgtgcgcc
      601 gcaccctgac cgccttcaac acggccaccg ccagcgagaa ccggatccac gccgatgacg
      661 tcgccagccg gttcggcttc accggcgggc tggtgccggg ggtcgatgtg tttgcctata
      721 tggcccacgc accggtcgcc cggtggggcc gggactggct gagccagggc cgcatccggg
      781 cgaaattcgg caagccggtc tatgacggcg acgagaccac ggtggcggcg gacgagcaac
      841 ccgacgggtc gctggccctg acggtgacgg cccgcggcct ggactgcgcc agcggaaccg
      901 cctggcgatc ggacgatgga gcggttcccg ccatcctgcc cgaggctgac ctgccccggc
      961 ccgaggaccg gccgccggcc tcgcccgaca gcctgccggt aggccgggtg ctgggcgccc
     1021 tgaaggagct ctatttcgcc gacgtcggcc tgtcgcacct gcagaacacg cgcgaggacc
     1081 cggcgctgtt cgacggcggc gacctctgca acccggccta catgctgcgc cgggccaact
     1141 atgtgctggc ccagtcggtg aagctggggc cgtggatcca tctggagagc gacgcgcgcc
     1201 tgcacggcct gatccgcgac ggcgaggcgc tggagacgcg cggcgtggtc gtggccaacg
     1261 aggagaagaa cgggcaccgg atcgtgacgc tcgatttcac ggtgtccagc cgcgaacgcc
     1321 tggccatgtc cgggaggcat tgggcgatct atgaaccccg gcaggtgcgg gagaaaggtt
     1381 aggccgcgac cgccgccagc ccgcgcgccc tcgcctccgc cggggagaac cacaccatcc
     1441 cgtcgcccca gtcgtggccg accgcgcccg tcggcggcgt cgcgctctcg ccgcagagga
     1501 aggccagctc ctggcccgtc agggtccccc agcgggccag cgcctccgcc ggcgtgggat
     1561 cgggacgagg atcggccgtg gcgc
//
LOCUS       DAORNX010000598         1581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_55212_length_1581_cov_6.261468, whole genome shotgun sequence.
ACCESSION   DAORNX010000598 DAORNX010000000
VERSION     DAORNX010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55212_length_1581_cov_6.261468"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..805)
                     /gene="ftsE"
                     /locus_tag="PLO65_17105"
     CDS             complement(71..805)
                     /gene="ftsE"
                     /locus_tag="PLO65_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRD30015.1"
                     /translation="MSRNRDAAPTGEPLAGPILRFDGVGMRYGRAAEVLKDVNFALAP
                     GSFHFLTGASGAGKSSLLKLIYLAERPSRGTIELFGRDVEAVAPADRPFLRRRIGVVF
                     QEFRLLDHLSAFDNVALPLRIAGGKPDAYRNDVAELLAWVGLKSRMHALPPTLSGGEK
                     QRLAIARAVVNRPDILLADEPTGNVDDAHALRILKLFVELNRLGTTVLIASHDEDLVA
                     RSGQPVLHLDKGRLRAFGRGAGAAGA"
     gene            860..>1581
                     /locus_tag="PLO65_17110"
     CDS             860..>1581
                     /locus_tag="PLO65_17110"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRD30016.1"
                     /translation="MILTCPECATRYFVPDDKVGPDGRTVKCSSCGNRWTAHGEPALE
                     LFVDAVEGATVRETAPAIEDQPVSALPGEELPKVFRQKADTSRRVREAMTTGVVWAGM
                     AVGLLALVATAIVFRVNVVRIWPGSAAAYAGVGLPVNRLGLTIENIGAEAVLKDSHAA
                     LAVTGVIRNVTDRPAAAPPLRVSLINKAGKTVAVKLAAPADPRIPPGGVRHFVVTLLD
                     PPTTAADLEVAFDTSGKAPPAPR"
BASE COUNT          240 a          554 c          567 g          220 t
ORIGIN      
        1 gagccgtcgc gcgggtcccg gcgtggcagc agcggcgcgg gcttccaccg ccggatgtcg
       61 aacagctcgc tcatgcgccg gcggcccccg cgccccgccc gaaggcgcgc agccggccct
      121 tgtcgagatg cagaaccggc tggcccgagc gggcgaccag gtcctcgtcg tggctggcga
      181 tcagcaccgt ggtgcccagg cggttgagtt cgacgaacag cttgaggatg cgcagggcgt
      241 gggcgtcgtc gacattgccg gtcggttcgt cggccagcag gatgtcggga cgattgacca
      301 ccgcacgggc gatcgccagc cgctgcttct cgccgcccga cagcgtcggg ggcagggcgt
      361 gcatgcgcga cttcaggccc acccaggcca gcagctcggc gacatcgtta cgataggcat
      421 ccggcttgcc cccggcgatg cgcagcggca gggcgacatt gtcgaacgcc gacagatggt
      481 cgagcagcct gaattcctgg aacaccaccc cgatgcgtcg gcgcaggaac ggccggtcgg
      541 ccggggccac ggcctcgacg tcgcggccga acagctcgat ggtcccgcgc gagggccgtt
      601 cggccaggta gatcagcttc agcaggctgc tcttgccggc cccggacgcg ccggtgagaa
      661 agtgaaacga gcccggcgcc agcgcgaagt tgacgtcctt gagcacctcg gccgcgcgac
      721 catagcgcat gcctacgccg tcaaagcgca gaatggggcc ggcgaggggc tcaccggtcg
      781 gtgcggcgtc tcggtttctg gacatgggag ccgaaatcgg cgacctcagt ggaggagcgg
      841 gagcgtccga ttcgccggca tgatactgac ctgtccagag tgcgctaccc gctatttcgt
      901 ccccgacgac aaggtcggac ctgacggacg gacggtaaag tgctcgtcct gcggcaaccg
      961 ctggaccgcc catggcgagc ccgccctgga actgttcgtc gacgccgtcg agggcgcgac
     1021 cgtccgcgag accgcccccg cgatcgagga tcagcccgtc agcgccctgc ccggcgagga
     1081 actgccgaag gtgtttcgtc agaaggccga caccagccgc cgggtgcgcg aggcgatgac
     1141 caccggcgtg gtctgggccg gcatggccgt cggcttgctg gccctggtgg cgacggcgat
     1201 cgtcttccgg gtcaacgtgg tgcggatctg gccgggcagc gcggcggcct acgccggcgt
     1261 cggcctgccg gtgaaccgcc tcggcctgac gatcgagaac atcggggccg aggcggtcct
     1321 caaggacagt cacgcggccc tcgcggtgac cggcgtgatc cgcaacgtca ccgaccgtcc
     1381 ggcggccgcc ccgccgctgc gcgtgagcct gatcaacaag gccggcaaga cggtcgcggt
     1441 gaagctggcc gcgccggccg atccccgtat tccacccggc ggcgtgcgcc atttcgtcgt
     1501 cacgctgctc gatccgccga ccactgcggc cgatctggag gtcgccttcg acaccagcgg
     1561 caaggcgccg ccggcgccgc g
//
LOCUS       DAORNX010000599         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_55758_length_1567_cov_8.079365, whole genome shotgun sequence.
ACCESSION   DAORNX010000599 DAORNX010000000
VERSION     DAORNX010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55758_length_1567_cov_8.079365"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLO65_17115"
     CDS             complement(<1..534)
                     /locus_tag="PLO65_17115"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168979.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRD30017.1"
                     /translation="MSSMNSVGRPTAPAQTNDYIHPTLTGARGLLQDEALIFELGGWD
                     KTGVDLPDADRPADDLDGLLRTAPIGLPGLSEPEAVRHYVRLSQKNHAIDLALYPLGS
                     CTMKHNPRLNEKAARMAGFADIHPLQPVSTVQGALALIDRLAHWLKTLTGMPAVAMTP
                     KAGAHGELCGLLAIRAAH"
     gene            complement(531..>1567)
                     /gene="gcvPA"
                     /locus_tag="PLO65_17120"
     CDS             complement(531..>1567)
                     /gene="gcvPA"
                     /locus_tag="PLO65_17120"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRD30018.1"
                     /translation="EIAQGTLQVLFEFQTQVAALTGMEIANASLYDGSTACAEAVMMA
                     QRVTRRDKAVLSGGLHPHYVRTTETLAHADGMDIVRLPAAVDAEDAVIAAIDSGTACV
                     VVQTPNVFGTATDVSKIAEAAHAAGALLIVVTTEAVSYGLLKSPGEMGADIAVAEGQS
                     IGNALNFGGPYVGLFACREKLIRQAPGRLCGETVDADGRRGFVLTLSTREQHIRRDKA
                     TSNICTNSGLCALAFSIHMSLLGEVGLRRLAALNHQTARALKTALEGVKGVEILTPRF
                     FNEFAVKLARPAAGVVEALAARGVLGGVPYSRLDPAAGLDDVLLVAATETVTEGDIAA
                     FKAALTEVMA"
BASE COUNT          210 a          526 c          595 g          236 t
ORIGIN      
        1 gtgcgccgcg cggatggcca gcaggccgca cagctcgccg tgggcgccgg ccttgggcgt
       61 catggccacg gccggcatgc cggtcagggt cttgagccag tgcgccagcc ggtcgatcag
      121 cgccagcgcg ccctgcacgg tcgagaccgg ctgcagcggg tggatgtcgg cgaagccggc
      181 catccgcgcg gccttctcgt tgaggcgcgg gttgtgcttc atcgtgcagc tgcccagcgg
      241 atagagggcc aggtcgatgg cgtggttctt ctggctcagg cgcacatagt ggcggaccgc
      301 ttccggctcg ctgagccccg gcaggccgat cggcgcggtg cgcagcaggc cgtcgagatc
      361 gtcggccggg cgatcggcat ccggcaggtc gacgccggtc ttgtcccagc cgcccagctc
      421 gaagatcagc gcctcgtcct gcagcaggcc gcgcgcgccg gtcagggtcg ggtggatgta
      481 gtcgttggtc tgggccggcg cggtgggacg gcccacggaa ttcatcgagc tcatgccatc
      541 acctcggtca gggcggcctt gaaggcggcg atgtcgcctt cggtcacggt ttcagtggcg
      601 gccaccagca ggacgtcgtc caggccggcg gcggggtcga ggcggctgta gggcacgccg
      661 ccgagtacgc ccctggcggc cagggcctcg acaacgccgg cggccggcct ggcgagcttc
      721 acggcgaact cgttgaagaa gcgcggggtc aggatctcga cccccttcac cccctccagc
      781 gccgtcttca gcgcgcgggc cgtctggtgg ttcagggccg ccaggcggcg caggccgacc
      841 tcgcccagca ggctcatgtg gatcgagaag gccagggcgc agaggccgct gttggtgcag
      901 atgttcgagg tcgccttgtc gcggcggatg tgctgctcgc gcgtcgacag ggtcagcacg
      961 aagccgcgcc ggccgtcggc gtcgacggtc tcgccgcaga ggcggccggg ggcctggcgg
     1021 atcagctttt cgcggcaggc gaacagaccg acgtacgggc cgccgaagtt cagggcgttg
     1081 ccgatcgact ggccctcggc gacagcgatg tcggcgccca tctcgccggg cgacttgagc
     1141 aggccgtagg acaccgcctc ggtggtcacc acgatcagca gcgcgcccgc ggcgtgggcc
     1201 gcctcggcga tcttcgatac atcggtggcc gtgccgaaca cgttgggggt ctggaccacg
     1261 acgcaggcgg tgcccgagtc gatggcggca atcaccgcgt cctcggcgtc gacggcggcc
     1321 ggcagacgca cgatgtccat gccgtcggcg tgggccaggg tctcggtggt gcggacgtag
     1381 tgggggtgaa ggccgccgga cagcaccgcc ttgtcccgcc gcgtgacccg ctgggccatc
     1441 atcaccgcct cggcgcaggc ggtcgagccg tcatacaggg aggcgttggc gatctccatg
     1501 ccggtcaggg ccgcgacctg ggtctggaac tcgaacagca cctgcagcgt gccctgggcg
     1561 atctcgg
//
LOCUS       DAORNX010000600         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_55761_length_1567_cov_6.311508, whole genome shotgun sequence.
ACCESSION   DAORNX010000600 DAORNX010000000
VERSION     DAORNX010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55761_length_1567_cov_6.311508"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLO65_17125"
     CDS             complement(<1..366)
                     /locus_tag="PLO65_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD30019.1"
                     /translation="MGQTTDRPGWRMALLATAVLALAGCGKAKEAPKAPQASQTVTVQ
                     AVAVVNVPRRVTASGDIVAWNEVVVGAETGGLTAVRVLVDEGAWVRQGQPLLQMNDDL
                     LRAQVRQQEANAQVSSGKTA"
     gene            534..>1567
                     /gene="lepA"
                     /locus_tag="PLO65_17130"
     CDS             534..>1567
                     /gene="lepA"
                     /locus_tag="PLO65_17130"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRD30020.1"
                     /translation="MTTPLSRIRNFSIVAHIDHGKSTLSDRLIQETGGLSAREMTAQV
                     LDSMEIEKERGITIKAQTVRLEYRADDGETYVLNLMDTPGHVDFAYEVSRSLAACEGS
                     ILVVDASQGVEAQTLANVYSAIDNNHEIVPVLNKIDLPAAEPERVRAQIEDVIGLDAS
                     DAVLCSAKSGVGIHEVLEAIVHRLPAPKGDPDAPLKALLVDAWYDAYLGVVVLVRVFD
                     GTLRAGQKIKMMQHGSTHLVDRVGVFKPKNTPVDSLGPGEIGFITAQIKEVADAAVGD
                     TLTDEKKPTTQALPGFKDVQPVVFCGLFPVDAADFEDLRAAVGRLRLNDGSFTYEMES
                     SAALGFGFRCG"
BASE COUNT          239 a          587 c          510 g          231 t
ORIGIN      
        1 ggcggtcttg ccgctcgaga cctgggcgtt ggcctcctgc tggcgcacct gggcgcgcag
       61 caggtcgtcg ttcatctgca gcagcggctg gccctggcgc acccaggcgc cctcgtcgac
      121 cagcacgcgc acggcggtca gcccaccggt ttcggcgccg accaccacct cgttccaggc
      181 gacgatgtcg ccgctggcgg tcacccgccg cggcacgttg accaccgcga ccgcctgcac
      241 cgtgaccgtc tggctggcct gcggcgcctt gggggcttcc ttggccttgc cgcaaccggc
      301 gagggccagc acggccgtcg cgagcagcgc cattcgccag ccgggccggt ctgttgtctg
      361 tcccacctgg tcgtcccctc gagccatgcc gtccggcgcc cgtcggcgag ccggcgaacg
      421 ttccctgcta tctatggtcc gcggcgctgt tcgcaagcgc gaaacgaccg aatccggttg
      481 cgcgtcgccc ccgcccctcg cgttggcgcg gcaatgtgct agacgcacgc agcatgacga
      541 cccccctgtc cagaatccgc aacttctcga ttgtcgccca catcgaccac ggcaagtcga
      601 cgctgtcgga ccggctgatc caggagaccg gcggcctgag cgcgcgcgag atgaccgccc
      661 aggtgctcga ctcgatggag atcgagaagg agcgcggcat caccatcaag gcccagaccg
      721 tgcggctcga ataccgggcc gacgacggcg agacctatgt cctgaacctg atggacaccc
      781 cgggccacgt cgacttcgcc tatgaggtca gccgctcgct cgccgcctgc gagggctcga
      841 tcctcgtcgt cgacgccagc cagggcgtcg aggcccagac cctggccaac gtctattcgg
      901 cgatcgacaa caaccacgag atcgtgccgg tcctgaacaa gatcgacctg ccggccgccg
      961 agcccgagcg cgtgcgggcc cagatcgagg acgtcatcgg cctggacgcc agcgacgcgg
     1021 tgctgtgcag cgccaagtcc ggcgtcggca tccacgaggt gctcgaggcc atcgtccacc
     1081 gcctgcccgc gccgaagggc gacccggacg cgccgctcaa ggccctgctg gtcgacgcct
     1141 ggtacgacgc ctacctgggc gtcgtcgtcc tcgtgcgggt gttcgacggc acgctgcgcg
     1201 cgggccagaa gatcaagatg atgcagcacg gctcgaccca cctggtcgac cgcgtcggcg
     1261 tgttcaagcc caagaacacc ccggtcgaca gcctcggccc gggcgagatc ggcttcatca
     1321 ccgcccagat caaggaggtt gccgacgcgg ccgtcggcga caccctcacc gacgagaaga
     1381 agcccaccac ccaggccctg cccggcttca aggacgtgca gccggtggtg ttctgcggcc
     1441 tgttcccggt cgacgcggcc gacttcgagg acctgcgcgc ggccgtcggc cggctgcggc
     1501 tcaacgacgg cagcttcacc tacgagatgg aatcgagcgc cgccctcggc ttcggcttcc
     1561 gctgcgg
//
LOCUS       DAORNX010000601         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56151_length_1557_cov_7.861518, whole genome shotgun sequence.
ACCESSION   DAORNX010000601 DAORNX010000000
VERSION     DAORNX010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56151_length_1557_cov_7.861518"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLO65_17135"
     CDS             complement(<1..1285)
                     /locus_tag="PLO65_17135"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD30021.1"
                     /translation="MHDLVIRNGRVIDGAETPAFTADIAIDGGRITAIGGIADAGRRE
                     IDASGLIVTPGWVDIHTHYDGQVTWDPYLSPSSWHGVTTAVMGNCGVGFAPVRRDRHD
                     WLIELMEGVEDIPGSALAEGISWDWETFPEYLDSLDAQARVLDVATQVPHSAVRAYVM
                     GERGARNEDATPEDIEAMAAIVAEGMKAGALGFSTSRTVVHTTKDGTVMPGTTAAADE
                     LLAIGRAMASVGPGVFEMSSDMAPAEPEFDWMRAMSTETGVTVTYSLLQSPLQPGNWI
                     KLLDLTRQARAQGASIAAQIACRPTGLVLGWQSTIHPFVGRQTYRDIAALPFEERLEK
                     LRDPAVRAAILADPSRKMGPLGAIMTQGFDRMFRLQQPDGGLDYEPRAEDSIAALAKA
                     SGQAPDAVVYDMLMENDGRGYIYLPLLNYTEFNFDH"
BASE COUNT          207 a          552 c          549 g          249 t
ORIGIN      
        1 tgtggtcgaa gttgaactcg gtgtagttca gcagcggcag gtagatgtag ccgcgcccgt
       61 cgttctccat gagcatgtca tagacgaccg cgtccggcgc ctggccgctc gccttcgcca
      121 gggcggcgat cgagtcctcg gcgcggggct cgtagtcgag gccgccgtcc ggctgctgca
      181 gccggaacat gcggtcgaag ccctgggtca tgatcgcgcc cagagggccc atcttccgac
      241 tcgggtcggc caggatggcg gcgcgcacgg cgggatcgcg cagcttctcg agccgttcct
      301 cgaacggcag cgcggcgatg tcacgatagg tctggcggcc gacgaacggg tggatggtgc
      361 tctgccagcc cagcaccagg ccggtcgggc ggcaggcgat ctgggcggcg atgctggcgc
      421 cctgggcgcg cgcctggcgc gtcaggtcca gcagcttgat ccagttgccc ggctgcaggg
      481 gcgactgcag caggctgtag gtcaccgtca cgccggtttc cgtcgacatg gcccgcatcc
      541 agtcgaactc cggttcggcc ggggccatgt ccgacgacat ctcgaacacc ccgggaccga
      601 cggaggccat ggccctgccg atggccagca gctcgtccgc cgcggccgtg gtgcccggca
      661 tcaccgtgcc gtccttcgtc gtatggacca cggtgcgcga ggtcgagaaa ccgagggccc
      721 cggccttcat gccctcggcg acgatggccg ccatggcctc gatatcctcg ggcgtggcgt
      781 cctcgttgcg cgcgccgcgc tcgcccatga cataggcccg caccgcgctg tgcggcacct
      841 gggtggccac gtcgagcacc cgcgcctggg catcgaggct gtcgaggtat tcggggaagg
      901 tctcccagtc ccagctgatg ccctcggcca gggccgaccc cgggatgtcc tccacccctt
      961 ccatcagctc gatcagccag tcgtggcggt cgcgtctgac cggcgcgaag ccgacgccgc
     1021 agttgcccat caccgccgtg gtcacgccat gccagctgga cggcgacagg taggggtccc
     1081 acgtcacctg gccgtcgtag tgggtgtgga tgtcgaccca ccccggggtg acgatcaggc
     1141 cggacgcgtc gatctcccgc cgtccggcgt cggcgatgcc gccgatggcg gtgatccggc
     1201 ctccgtcgat ggcgatgtcg gccgtgaagg ccggcgtctc cgcgccatcg atcacccgtc
     1261 cgttacggat caccagatca tgcatcgcct gtccccatcg ccggggagtc cgtcctggag
     1321 gggacggctc ccggtgtcgc gggcgatcat gaccgcttgc accggccgcg gaaagtatga
     1381 cccagcgtca cgaccggacc gcgcctgtcg ggggcagagt ctcgacgacc ggccggtaac
     1441 gtgtgacatc cgggccggat gagcgagcac cttcccttcg tcggaccggg gcggccctgg
     1501 cggtccgccg ggcggttcgc cgccgccttc gccgccttcg cgggcccgcg cggctgg
//
LOCUS       DAORNX010000602         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56396_length_1551_cov_5.281417, whole genome shotgun sequence.
ACCESSION   DAORNX010000602 DAORNX010000000
VERSION     DAORNX010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56396_length_1551_cov_5.281417"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLO65_17140"
     CDS             <1..202
                     /locus_tag="PLO65_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRD30022.1"
                     /translation="VAPGGPPPGPLGEIRLESRGRGLGLIGVTTRLAAPQEVTLDELR
                     LEAFLPADDESETMLLALAGGV"
     gene            307..1155
                     /locus_tag="PLO65_17145"
     CDS             307..1155
                     /locus_tag="PLO65_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRD30023.1"
                     /translation="MRAFLTIGAATAALLGATAAQAASVEIKDAAARVVVIPEDRSDV
                     KVEFLTTSPDLPLTVRTEGDKTIVDGDLRRNAIHNCRTFNGQTSVTVRGKTFGWDALP
                     QVVVRVPMDARVAGGGAVFGSIGRTNSLTLSNAGCGDWTVGNVAGELNINQAGSGDTT
                     VGEAGKTSINIAGSGDVATAGLSGGLSVNIAGSGDVSAASVTGPVAANIMGSGDVDVR
                     GGRVGEVSVSIAGSGDVIIMAPADSVSAKIAGSGDVRVTSVSGGVSKKVAGSGEVIVG
                     PIDSDD"
     assembly_gap    1234..1243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          220 a          557 c          564 g          200 t
ORIGIN      
        1 cgtggcgccg ggcgggccgc cgcccggtcc gctgggcgag atccgccttg aatcccgggg
       61 ccgcgggctg ggcctgatcg gcgtgaccac ccggctggcc gcgccccagg aggtgaccct
      121 ggacgagctg cgcctggaag ccttcctgcc ggccgacgac gaatccgaga cgatgctgct
      181 ggcgctggcc ggcggggtct gacggcgtcc gaccatgaca gctctgtcat gacctcgaat
      241 taagctgccc cgcggccccg gcgggcctag cgtccggcca tcacgcattg aggggagaag
      301 aatccgatgc gcgctttcct gaccatcggc gccgcgaccg cggcgctgct cggcgccacc
      361 gccgcccagg ccgcctccgt ggagatcaag gacgcggccg cccgcgtcgt ggtcatcccg
      421 gaagaccgga gcgacgtgaa ggtcgagttc ctgaccacca gtccggacct gccgctgacg
      481 gttcgcaccg agggcgacaa gaccatcgtt gacggcgacc tgcgccgcaa cgccatccac
      541 aactgccgca ccttcaacgg ccagaccagc gtcacggtcc ggggcaagac cttcggctgg
      601 gacgccctgc cgcaggtggt cgtccgcgtg ccgatggacg ccagggtggc cggcggcggc
      661 gcggtgttcg gcagcattgg ccggaccaac agcctgaccc tgtccaacgc cggctgcggc
      721 gactggacgg tcggcaacgt cgccggcgaa ctgaacatca accaggccgg gtcgggcgac
      781 accaccgtgg gtgaagccgg caagacctcg atcaacatcg ccggctcggg cgacgtggcc
      841 acggccgggc tctccggcgg cctgtcggtc aacatcgccg gctcgggcga cgtcagcgcc
      901 gcctcggtca ccggtccggt cgcggccaac atcatgggct cgggcgacgt cgacgtccgc
      961 ggcggccggg tcggcgaggt ctcggtgtcg atcgccggct ccggcgacgt gatcatcatg
     1021 gccccggccg acagcgtcag cgccaagatc gccggctccg gcgacgtgcg ggtgaccagc
     1081 gtgtctggcg gcgtgagcaa gaaggtcgcc ggctcgggcg aggtgatcgt cggcccgatc
     1141 gactccgacg actgactcga aaccccccga acaggggata cgcggccggt cgcgcctgcc
     1201 tagggtgagg ccgtgcggaa ctccccggcc ggtnnnnnnn nnncgtcgtt gacgcaccgg
     1261 ccgggcgcgg gcttgcccgc ctccggccgg cgctgtccct ccaagggcgt cgggcggcag
     1321 gagttccgcc acctgggagg gccatcatcg ggccgatgcg accgacggat atggaaaagc
     1381 cccggagcca tgagctccgg ggctttttcg ctgcgctcgc ccgatcccgg gggaggtggc
     1441 gggcggcgcg gcgatggcgg cgccacgggc gaacccgcga cgccgccccg tccgggacgt
     1501 cctactgcag gacgttgaac aggccggccg cggcgccgag gtccgcgccc g
//
LOCUS       DAORNX010000603         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56399_length_1551_cov_3.391711, whole genome shotgun sequence.
ACCESSION   DAORNX010000603 DAORNX010000000
VERSION     DAORNX010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56399_length_1551_cov_3.391711"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>256
                     /locus_tag="PLO65_17150"
     CDS             <1..>256
                     /locus_tag="PLO65_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30024.1"
                     /translation="NDAPDLALRTEAHQAAWERAVEHIAAREPAVQGRLAFYRDSLAY
                     HRGELDGVPLRCLGLVDQYGVTYEGDLLPCCVWGGEGLKVG"
     assembly_gap    257..460
                     /estimated_length=204
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          222 a          477 c          447 g          201 t
ORIGIN      
        1 aacgacgccc ccgacctcgc cttgcggacg gaggcccacc aggccgcctg ggagcgggcc
       61 gtcgagcaca tcgccgcgcg ggagcccgcc gtccagggcc ggctcgcctt ctaccgggac
      121 tcgctggcct accaccgcgg cgagctcgac ggagtcccgc tgcgctgtct tgggttggtc
      181 gatcagtacg gggtcaccta cgaaggcgat ctgctgccct gctgcgtctg ggggggagag
      241 ggcctcaagg tcggcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggggggaggg ttgattggtc
      481 ttctccctcc cctcgctcgt gctcatcatt gcccacatct ttccgggcca agggcgaggg
      541 gcaacgacag cgacctcggc ttgcggagcc gccaaggcca ggtggtcgaa cggaggggag
      601 gtcgacgggg aaggccagga ggcggagccg ccttcattga gagtggcccc gggcggagtg
      661 gtagcggatg agagttcaac gcccacgcgc ggccttctac gagcgagttc gaccctcagc
      721 accctcggtt cggcattgcc gacccgcacg actctcaccc aggtcgactc cgccatcgcc
      781 ggcccacacc ctcccgctca catcgccacg gacctcgtgg cgtcctggcg tcttggcgtc
      841 tcctccgccc cctacgaccc gggccgaaca ccagggacgc cgccccgacc tgacccgtgt
      901 gccagcttgc gaggaagcgc tgggctgtgt tcctcagccg gccggtgaag gaccggccat
      961 caggaagaga ggaaggcagg gcaggaagga agacagagtg gagccatctc cttggtgttc
     1021 accgaggctc cgtggtggat ggccgttgcc gaaggatgcg ccgacctggc cgcccaaccc
     1081 ctgcgttcct gcaacccatc gcggtcaacc ccctcatttc ttcaacctcg aacggcccgc
     1141 agccccgtaa ctccaacaaa cacgtctccg aggctggcgc tccacctggc agcactccca
     1201 tggggttgca aattggattg ggttgacctg gactggttgc aaatccgccg ggcttgacag
     1261 gtcagcggcg ccaccgcagg cgcggatcct gggcgagcag gccacagaga cgaccatcgg
     1321 cgtcatcccg cgaccggacc accccttcgt cgcaccgaac cggcgcaacg aggccgactc
     1381 cgccgccgac gtccgcaagt ccacctccat caaccagcgc ggccagcccc gcaagctgcg
     1441 ggcggggctc acccgagcga cttgtgggta atgatgagcg cgagcggggg gaggtgccga
     1501 gcgcagcgag gcggaggggg gtcgtcctgc ccccccccgc tgttttcaga t
//
LOCUS       DAORNX010000604         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56653_length_1545_cov_7.840268, whole genome shotgun sequence.
ACCESSION   DAORNX010000604 DAORNX010000000
VERSION     DAORNX010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56653_length_1545_cov_7.840268"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..370)
                     /locus_tag="PLO65_17155"
     CDS             complement(5..370)
                     /locus_tag="PLO65_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30025.1"
                     /translation="MRTAVTGLLVSLMAASAVSAKPDFRAQARCIAAYEVAAGLLEAD
                     ADAASGADKVLIRKRIDKLDANRERLSLSLATRTDLTPADSKASQQERKAEEARLDKL
                     APAAVQAAADACDGIVSAG"
     gene            complement(473..>1545)
                     /locus_tag="PLO65_17160"
     CDS             complement(473..>1545)
                     /locus_tag="PLO65_17160"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRD30026.1"
                     /translation="WNFPVNLTFAPLAGILAAGNRAMIKPSEFTPATSELMKTMFAKA
                     FSEEEIAVITGGPEVGQAFAGLPFDHMIFTGATSIARHVMRAAAENLVPLTLELGGKS
                     PVIISRSADMATAAARIMNGKTLNAGQICLAPDYVMAPEGRLEEFVTEASSAVARMFP
                     TLKDNPDYTAVVAQRHYDRIKGYIDDARAKGADVREINPAGEDFSQQEHRKIPPTLIL
                     NATDDMTVMQDEIFGPVLPVKTYKAVGEAVDYINAHERPLGLYWFGSDEAEKERVLNH
                     TTSGGVTVNDVIMHVAQEELPFGGVGPSGMGSYHGHDGFLEFSHRKAVYTQLKKDIGP
                     LLAMRPPYGPAIRKYLAGTLSK"
BASE COUNT          231 a          515 c          527 g          272 t
ORIGIN      
        1 gggatcaccc ggcgctgacg atcccgtcgc aggcgtccgc cgcggcctgc accgccgcgg
       61 gggccagctt gtccagccgg gcctcctcgg ccttgcgctc ctgctggctg gccttgctgt
      121 ccgccggcgt caggtccgtg cgggtggcca gggacaggga cagccgctca cggttcgcgt
      181 cgagcttgtc gatgcgcttg cggatcagga ccttgtccgc gccgctggcg gcgtcggcgt
      241 cggcctcaag caggccggcg gccacctcgt aggccgcgat gcaacgggcc tgggcccgaa
      301 agtcaggctt cgccgagacg gccgaagccg ccatcagcga aaccagcagg ccggtcacgg
      361 ccgtacgcat gctcatctcc actgggccag agggccctca ggcgggatat cgtatgccgc
      421 cactgtagct gcaatacggc gccccgcctg acggccagat gaacagcctc gcctatttcg
      481 agagcgtgcc ggccagatac ttgcggatcg ccggtccgta gggcggacgc atcgccagca
      541 gcggaccgat gtccttcttg agctgggtgt agaccgcctt gcggtggctg aactcgagga
      601 agccgtcgtg gccgtgatag ctgcccatgc ccgacgggcc gacaccgccg aacggcagct
      661 cctcctgggc cacgtgcatg atcacgtcgt tgaccgtcac cccgccgctg gtcgtatggt
      721 tgagcacccg ctccttctcg gcttcgtcgg agccgaacca gtagagaccg agcgggcgct
      781 catgggcgtt gatgtaatcg accgcctcgc cgacggcctt gtaggtcttc accggcagca
      841 caggaccaaa gatctcgtcc tgcatcaccg tcatgtcgtc ggtggcgttg aggatcaggg
      901 tcggcggaat cttgcgatgc tcctgctggc tgaagtcctc gccggcgggg ttgatctcgc
      961 ggacgtcggc gcccttggcc cgggcgtcat cgatatagcc cttgatccgg tcatagtgcc
     1021 gctgggcgac gacagcggtg tagtcggggt tgtccttcag ggtcgggaac atgcgggcaa
     1081 ccgcgctgga ggcctcggtg acgaattcct cgagccgccc ctcaggcgcc atcacatagt
     1141 ccggcgcgag gcagatctgg ccggcgttga gcgtcttgcc gttcatgatc cgagcagcgg
     1201 cggtggccat gtcggcgctg cgggagatga tcaccgggct cttgccgccc agttccagcg
     1261 tcagcggcac caggttctcg gccgccgccc gcatgacatg acgggcgatc gaagtcgccc
     1321 cggtgaagat catgtggtcg aacggcaggc cggcgaaggc ctggccgacc tccggcccgc
     1381 cggtgatcac ggcgatttcc tcctcgctga aggccttggc gaacatggtc ttcatcagct
     1441 cggaagtcgc cggggtgaac tcggacggct tgatcatcgc cctgtttccg gccgccagga
     1501 tgccggccag cggcgcgaag gtcaggttga ccgggaagtt ccacg
//
LOCUS       DAORNX010000605         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56655_length_1545_cov_4.065101, whole genome shotgun sequence.
ACCESSION   DAORNX010000605 DAORNX010000000
VERSION     DAORNX010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56655_length_1545_cov_4.065101"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>428
                     /locus_tag="PLO65_17165"
     CDS             <1..>428
                     /locus_tag="PLO65_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD30027.1"
                     /translation="DMTDKKSDNETTKPGSAPAAATVQDYVDCITEQEVIRRQWDAFF
                     AAFDVVIAPCYATPAFVHFDEPDPWPGLNRTLDIDGAEVAYAPQHAWPLFAGMAQLPA
                     TVAPIGRTRGGLPIGAQIIGPFLEDLTPIALLTAVTPTQGP"
     assembly_gap    429..453
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(469..554)
                     /locus_tag="PLO65_17170"
     tRNA            complement(469..554)
                     /locus_tag="PLO65_17170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(519..521),aa:Leu,seq:caa)
     gene            complement(596..1090)
                     /locus_tag="PLO65_17175"
     CDS             complement(596..1090)
                     /locus_tag="PLO65_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRD30028.1"
                     /translation="MGADVTPLPRKRSPDTLVLDDYLPYRLSIASNGVSGLIARAYQD
                     RFGLSIPQWRLMAVLAEGALTPQALVARTAMDKVTVSRAAQGLVARNLVVRTDSPSDG
                     RSHTLALSDTGRRLHGEIAPLALAYEAALLAGLAPAEVATLKRLLARLEAAAARLSGE
                     PPAR"
     gene            complement(1108..>1545)
                     /locus_tag="PLO65_17180"
     CDS             complement(1108..>1545)
                     /locus_tag="PLO65_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD30029.1"
                     /translation="PASAFSPVAVTPDSLGDRWKDGRLHGALEVELNGKPFGEADAGV
                     DMTFDFGTLIAHAAKTRALGAGTIIGSGTVSNRDADGGPGKPISEGGLGYSCIAEVRT
                     VETLLAGEAKTPFLRHGDTVRIEMRDGKRHSIFGAIEQTVAPA"
BASE COUNT          221 a          539 c          540 g          220 t
ORIGIN      
        1 gacatgacag acaaaaaatc ggacaacgag acgaccaaac ccggttcggc tcccgccgcc
       61 gcgaccgtcc aggattatgt cgactgcatc accgagcagg aggtgatccg gcgacagtgg
      121 gacgccttct tcgccgcctt cgacgtggtc atcgccccct gctacgccac gccggccttc
      181 gtccatttcg acgagccgga tccgtggccc gggctgaacc gcaccctgga catcgacggc
      241 gccgaggtgg cctacgcccc gcagcacgcc tggccgctgt tcgccgggat ggcccagctg
      301 ccggcgacgg tcgccccgat cggccgcacc cgcggcggcc tgccgatcgg ggcgcagatc
      361 atcggcccgt tcctcgaaga cctgacgccg atcgcgctgc taaccgccgt cacgccgaca
      421 cagggcccnn nnnnnnnnnn nnnnnnnnnn nnncccgtaa caggccgatg gtgccccagg
      481 ccggactcga accagcactt ccttgcagaa agcagatttt gagtctgccg cgtctaccat
      541 ttcgccactg gggccagcgc gaggagcggg caatctactg acgcaggggc tcaggtcaac
      601 gcgccggcgg ttcgccggac agtcgggcgg cggcggcctc gaggcgggcc agcagacgct
      661 tgagggtggc cacctcggcc ggggccaggc cggccagcag ggcggcctcg taggccaggg
      721 ccaggggcgc gatctcgcca tgcagccggc ggccggtgtc gctcagggcc agggtgtgcg
      781 agcgcccgtc cgacgggctg tcggtgcgga ccaccaggtt gcgggcgacc agcccctggg
      841 cggcgcggct gacggtgacc ttgtccatcg ccgtgcgggc gaccagggcc tggggcgtca
      901 gggcgccctc ggccagcacg gccatcagcc gccactgggg gatcgagaga ccgaagcggt
      961 cctggtaggc ccgggcgatc aggcccgaga cgccgttgga ggcgatcgac aggcggtagg
     1021 gaaggtagtc gtcgagcacc agcgtgtcgg gcgaccgctt gcgcggaagc ggcgtcacgt
     1081 cagcgcccat ggcttttttc cttcccacta cgcgggcgcc accgtctgtt cgatggcccc
     1141 gaagatcgag tggcgcttcc cgtcgcgcat ttcaatgcgg acggtgtcgc catggcgcag
     1201 gaagggcgtc ttcgcctctc cggcgagcag cgtctcgacc gtgcgcacct cggcgatgca
     1261 gctgtagccg aggccgcctt cgctgatcgg cttgcccggg ccgccgtcgg cgtcgcggtt
     1321 ggacaccgtg cccgatccga tgatcgtgcc ggcgcccagg gcgcgggtct tcgccgcgtg
     1381 ggcgatcagg gttccgaagt cgaaggtcat gtcgaccccg gcgtcggcct cgccgaacgg
     1441 cttgccgttc agctcgacct ccagggcgcc gtgcagccgg ccgtccttcc agcggtcgcc
     1501 cagcgagtcc ggcgtgaccg cgaccggcga gaaggcgctg gccgg
//
LOCUS       DAORNX010000606         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56850_length_1540_cov_4.488215, whole genome shotgun sequence.
ACCESSION   DAORNX010000606 DAORNX010000000
VERSION     DAORNX010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56850_length_1540_cov_4.488215"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLO65_17185"
     CDS             complement(<1..673)
                     /locus_tag="PLO65_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD30030.1"
                     /translation="MIDISVTGVGGQGDGLAPGPLFVPLTLAGERVRARRDGQRADLV
                     ELLEASPERVTPPCPHFGLCGGCALQHWDAAPYLAWKGDLVRTALSHEQIETDILPTF
                     ASPPGSRRRLALHARGGKGGVRLGFKERRSWSLVDIETCVIADPRLVSALPTLRRLAR
                     PFLEHPKSAPTLHVTWTATGLDVDVTGVERKSGGLSADARMRAAEAASAGDMARVTLA
                     GEMVYQ"
     gene            863..1282
                     /locus_tag="PLO65_17190"
     CDS             863..1282
                     /locus_tag="PLO65_17190"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRD30031.1"
                     /translation="MRTILTLSAALAIAAPAFVAPTFAEAQSSRSYGRGDVCAYKRQQ
                     AKKKGTIAGALIGGTLGAAVGARGNKTEGAVLGGTVGAIAGNQIARDNFHCSNYPRRV
                     SARRGSCKWVQEYYGGRWHGFEVCRGRDGYWRPSGRG"
     gene            1328..>1540
                     /locus_tag="PLO65_17195"
     CDS             1328..>1540
                     /locus_tag="PLO65_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30032.1"
                     /translation="MTTRSILNGGVAGLAALAMIAGAAAIPTAASAQGYAPSGAYEGG
                     YRYDGCQRERTTRGTGGALVGAGLGAL"
BASE COUNT          225 a          576 c          527 g          212 t
ORIGIN      
        1 cctggtagac catttccccg gccagggtga cgcgggccat gtcgccggcc gaggccgcct
       61 cggcggcgcg catgcgggcg tcggccgaca gcccgccgct cttgcgctcg accccggtga
      121 cgtcgacgtc caggccggtc gccgtccagg tgacatgcag cgtcggcgcc gacttggggt
      181 gctcgaggaa cggccgggcc agccgccgca gggtcggcag ggccgacacc aggcgtggat
      241 cggcgatcac gcaggtctcg atgtcgacca gcgaccagct gcgtcgctcc ttgaagccca
      301 gccggacccc gcccttgccg ccccgggcgt gcagggccag ccgccggcgc gagcccggcg
      361 gcgaggcaaa ggttggcagg atgtcggtct cgatctgctc gtggctcagc gccgtccgga
      421 ccaggtcgcc cttccatgcc agatagggcg ccgcgtccca gtgctgcagg gcgcagccgc
      481 cgcagagacc gaagtgcgga cagggcggcg tcacccgctc ggggctggcc tcgagcagtt
      541 cgacaaggtc ggcccgctgg ccgtcgcgac gcgcccgcac cctttccccc gcaagggtca
      601 gcggcacgaa cagcgggccc ggcgccaggc cgtcgccctg cccgcccacg cctgtgacgg
      661 atatgtcgat caggtcggcc aaccgactct cctccgggaa ccacgatcct ccgttatcat
      721 tgatgcgggg cgacatggcg gccaatcggc cggcatgtga acgaagatga acgggtcgct
      781 caacggccgt tcaggttgga cccgccatct tctcaccatc aacggcgacg aatactcgcc
      841 ccagaagcga agggagcgtg tcatgcgcac cattctcact ctttccgccg ccctcgcgat
      901 cgccgccccg gccttcgtcg cgccgacgtt tgccgaagcc cagtcgtcgc gcagctatgg
      961 ccgtggcgat gtctgcgcct acaagcgcca gcaggccaag aagaagggca ccatcgccgg
     1021 cgccctgatc ggcggcaccc tcggcgccgc ggtcggcgcg cgtggcaaca agaccgaggg
     1081 cgcggttctc ggcggcacgg tcggcgctat cgccggaaac cagatcgccc gcgacaattt
     1141 ccactgctcc aactatccgc gccgcgtcag cgcccgtcgc ggcagctgca agtgggtcca
     1201 ggaatattac ggcggtcgct ggcacggctt cgaggtgtgc cgcgggcgtg acggctactg
     1261 gcggccgagc ggccggggct agaccccgcc cccggccccg catcatcgag atcaagggag
     1321 agctttcatg accacccgtt ccatcctcaa cggcggcgtc gccggcctgg ccgccctggc
     1381 catgatcgcc ggggccgccg ccatcccgac ggcggcttcg gcccagggct acgccccgtc
     1441 tggcgcctac gagggcggct atcgctacga cggctgccag cgtgagcgga ccacccgggg
     1501 aacgggcggc gccctggtcg gcgccggcct cggcgccctc
//
LOCUS       DAORNX010000607         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56888_length_1539_cov_7.030323, whole genome shotgun sequence.
ACCESSION   DAORNX010000607 DAORNX010000000
VERSION     DAORNX010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56888_length_1539_cov_7.030323"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLO65_17200"
     CDS             <1..1314
                     /locus_tag="PLO65_17200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD30033.1"
                     /translation="TPMIHRPSIVAGLIAALLAAEPFSTAAAPIPSPDSPRVQLPGTS
                     PAQGKVDAGLKALNAGELVAAEAAFTEAARVDPKLSAAYIGLAEVAARQNKPARVEYW
                     LKQALSVDPNGALTQRTWGRYLFQQGRFVEAEVTFKKAVALDPRSVDAQLNLAENSLL
                     GLKKAKPAEDAYRAAIALDAGNPQAHAGLAATLAAQGHPDAALAEYEQAVRLAPADPK
                     FAHALARFLASQGKLDQALAAQQKIIAMAPDFFPAYIDLGDLYLARGDLDQAANTYRS
                     ATKVTKNPALAFFKLGTVLEGQQRWAEAEQAYLEAVRNDPMMYGAYNNLAFMAAVRKE
                     RLDDALVWARKAAELAPKVTTIQDTLGWVHRARGELGPAAQAIEKAVAANPKKAGFRY
                     HLGVVYAEQGKKKEAVAALQKALELDQNFNHAADARQRLQQLSAK"
BASE COUNT          298 a          531 c          480 g          230 t
ORIGIN      
        1 actcccatga ttcatcgacc atcgatcgtc gccggactga tcgctgccct gttggcggca
       61 gaaccgttct cgaccgcggc agcacccatt ccgtcgccgg attccccgag ggtacagctc
      121 cccggcacgt ctcccgcaca gggtaaggtg gacgccggcc tgaaggcatt gaacgccggc
      181 gagctggtgg ccgccgaggc cgcattcacg gaagcggcca gggttgatcc caagctgtcc
      241 gcagcctaca tcggtctggc ggaagtcgcc gcacggcaaa acaagcccgc ccgcgtggaa
      301 tactggctga agcaggcgct gtcggtcgat ccgaacggcg cgctgacgca gcgcacctgg
      361 ggccgctacc tgtttcagca agggcgcttc gtcgaagccg aggtcacttt caagaaggcg
      421 gtcgcgctcg acccacgctc ggtggacgct cagctcaacc tcgccgagaa ctccctgctt
      481 ggcctcaaga aggcaaaacc ggcagaagac gcctaccgtg cggccatcgc gctcgacgcc
      541 ggcaatccgc aggcgcatgc gggtctggcc gcgacgctgg cggcgcaagg ccacccggat
      601 gcggcactcg ccgagtatga gcaggcggtc cggcttgcgc ctgccgaccc gaagttcgcg
      661 cacgccctcg cccgcttcct ggcgtcccag ggcaaactcg atcaggctct ggccgcacag
      721 cagaagatca tcgccatggc gccggatttt ttccccgcct acatcgatct cggcgacctg
      781 tatctggcca ggggcgacct cgaccaggcc gccaacacct atcgctccgc caccaaggtg
      841 accaaaaacc cggcgctggc cttcttcaaa ctcgggaccg tgctcgaagg ccagcagcgc
      901 tgggccgaag ccgaacaggc ctacctcgag gccgtgcgga acgacccgat gatgtatggc
      961 gcgtacaaca atctcgcctt catggccgcg gtgcgcaagg agcgtctcga cgacgcgctg
     1021 gtctgggcca ggaaggcggc tgaactggcg cccaaagtga cgacgatcca ggacacgctt
     1081 ggctgggtcc accgggcccg tggtgaactc ggcccggccg cgcaggcgat cgagaaggcg
     1141 gttgccgcca accccaagaa ggccggtttc cgctaccacc tcggggtggt ctatgccgag
     1201 cagggcaaga agaaggaagc ggtcgccgcg ctgcagaaag cactcgaact cgaccagaac
     1261 ttcaaccacg cggccgacgc cagacagcgc ctgcagcaac tctcggcgaa gtagcgagcg
     1321 cgggcggccg aggtatgctg ctccccaaaa gaaaagcggc ccgcaggccg cttttcattc
     1381 tgccctaccg attcgtttaa tctgaaagtc gcatatctcc cctctccact cgctggagag
     1441 gaaaacgtca gactgaacgg cgacggcgcg ctgcccccag gccgagcagg cccatgccca
     1501 tcaggaccag tgtggccggc tcgggagtct tgaagctgt
//
LOCUS       DAORNX010000608         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_56924_length_1538_cov_7.641942, whole genome shotgun sequence.
ACCESSION   DAORNX010000608 DAORNX010000000
VERSION     DAORNX010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56924_length_1538_cov_7.641942"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLO65_17205"
     CDS             complement(<1..516)
                     /locus_tag="PLO65_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD30034.1"
                     /translation="MSAAAVDATPLSLVLPGLTGLLQQAADAAGAFVTAARGEVRAQV
                     SGGGKIDRVLADVEQHRVHGFGWYASYAELMNQVAGWAARLEAEGAFGETEALLAQLL
                     FAEYCTQLVGGIPMNQGEMVRPADLTASRETMDRLFAPALVQLMTQGGGQAVKTRLAD
                     LVAAARGKPTVE"
     gene            661..1287
                     /locus_tag="PLO65_17210"
     CDS             661..1287
                     /locus_tag="PLO65_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD30035.1"
                     /translation="MLVVHHLNESRSQRILWLLEELGVPYEIRFHARDATTRLAPPEL
                     RTIHPLGKSPVITDGDNTVIESGAIIDYIIRRHGSGRMAPAPGTADHEAYLQWLHYGE
                     GSAMLPLMLNMYVMRLGEAGAPLHPRIESEIANHMSYIEGALAGRDWLVGDGPTGADA
                     QMSFIPEVLKAFGKLGDYPNMAAWIDRIHARPAWQAGLDKGGPYLMGR"
BASE COUNT          251 a          532 c          518 g          237 t
ORIGIN      
        1 ctcgaccgtc ggcttgccgc gcgcggccgc gacgaggtcg gcgaggcggg tcttgaccgc
       61 ctggcccccg ccctgggtca tcagctggac cagggccggc gcgaacaggc ggtccatcgt
      121 ctcgcggctg gccgtcaggt cggccgggcg gaccatctcg ccctggttca tcgggatgcc
      181 gccgaccagt tgcgtgcagt attcggcgaa cagcagctgg gccagcaggg cctcggtctc
      241 gccgaaggcg ccctcggctt cgagccgcgc ggcccagccg gcgacctggt tcatcagctc
      301 ggcatagctg gcgtaccagc cgaagccgtg gaccctgtgc tgttcgacat cggccagcac
      361 gcggtcgatc ttgccgccgc cggaaacctg cgccctcacc tcgccacggg ccgcggtcac
      421 gaacgcgccg gcggcgtcgg ccgcctgctg caacaggccg gtcaggcccg gaagaaccag
      481 tgagaggggc gtggcatcga cggcggcggc gctcatgggt tcgttctccc cgaaatttgt
      541 tgcggcgcac tatatgcatt tcgcacacgc acacaatcag agccgggaaa ggtagaattt
      601 gccacgtcca aggcgagccc tatgttgcgt tccagccata ttttttcggg ggaaaccgcc
      661 gtgctcgtcg tccatcatct caatgagtcg cgctcgcagc gcatcctctg gctgctggag
      721 gagctgggcg tcccctacga gatcaggttc cacgcccgcg acgcgaccac ccgcctggcg
      781 ccgcccgaac tcaggacgat ccacccgctg ggcaagtcgc cggtcatcac cgacggcgac
      841 aacacggtca tcgagtcggg cgcgatcatc gactacatca tccgccgtca cgggagcggt
      901 cgcatggccc ccgcccccgg cacggccgac cacgaggcct atctgcagtg gctgcactac
      961 ggcgagggca gcgccatgct gccgctgatg ctgaacatgt acgtcatgcg gctgggcgag
     1021 gccggcgcgc cgctgcaccc gcgcatcgaa agcgagatcg ccaaccacat gagctacatc
     1081 gagggcgcgc tggccggccg cgactggctg gtcggcgacg gcccgaccgg tgccgacgcc
     1141 cagatgagct tcatccccga ggtgctgaaa gccttcggca agctcggcga ctacccgaac
     1201 atggccgcct ggatcgaccg catccacgca cgccccgcct ggcaggcagg tctggacaag
     1261 ggcgggccgt atctgatggg gcggtgagcc tcggggacat gtacctttgg tacgtgtccc
     1321 ctatccgagc ggcttgagta ggggacacgt accaaaggta catgtcccct agggcagcgg
     1381 atcgtccaga tagccgtcga ggaagtcgaa taccgtcgcc ttgagcagac cgtgcggaat
     1441 ggcgttgaag tggtcgcagc cgatcaggct gacggcccgg gcgccgggga tcgcgtcggc
     1501 caggtcctgc gggtcgccgg ccagggcgtc ccgggccc
//
LOCUS       DAORNX010000609         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57162_length_1533_cov_5.851150, whole genome shotgun sequence.
ACCESSION   DAORNX010000609 DAORNX010000000
VERSION     DAORNX010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57162_length_1533_cov_5.851150"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLO65_17215"
     CDS             complement(<1..195)
                     /locus_tag="PLO65_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30036.1"
                     /translation="MPQTAPAEAGYAIVVQAEQDHWIWALMDLDAVVAASGSAANRDS
                     AWRCGSLAAATFAALQRARRR"
     gene            complement(290..>1533)
                     /locus_tag="PLO65_17220"
     CDS             complement(290..>1533)
                     /locus_tag="PLO65_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD30037.1"
                     /translation="ADPAALAVLPALRPVIDETTRVTLASVVLLDRNGTVVQGLGLGG
                     SLRRLPEVAAALRGETRTVLRRQSDYHPRYSFEWLSRASGIRLHHARPILVNGRVEGV
                     LLLSRSPRALFSGLYEDRGKFLIGLAGLILILVLLSGLVSRGITRPIVALSRASRDVA
                     AGQGDIPQTPQTAAVEIRQLYEDFRAMAEAIARRSRYLRDFAAAVSHEFKTPLAGIGG
                     AVELLQDHGAAMSPEERSRFLANISADNARLTQLVTRLLDLARADMARPEAGAAADVE
                     GSVRRVVDSLDDNPRVDVALPRDLPMVGVPSEAIETVLGVLLVNSRQAGAGKVIISGA
                     AGERAVVLTVTDDGPGVAEADRERLFEPFFTSRRAQGGTGLGLPIARSLLAASDGRIS
                     LTPSAVGARFEVVLPIADTAG"
BASE COUNT          235 a          557 c          546 g          195 t
ORIGIN      
        1 gcgccggcgc gcgcgttgaa gggcggcgaa ggtcgcggcc gccagcgacc cgcaacgcca
       61 ggcgctgtcg cggtttgcgg ccgatccgct ggcggcgacg accgcgtcca gatccatcag
      121 ggcccagatc cagtggtcct gctcggcctg cacgacaatg gcgtagcccg cctcggcggg
      181 cgcggtctgc ggcataacgg atcctcccct gttttcccga ggctggaccg gccgcgccga
      241 acggtgaggg aggcgatgtg gagaagggat gcaggcggcg gcgggctgtt cagcccgcgg
      301 tgtccgcgat gggcagcacg acctcgaaac gcgccccgac ggcggatggc gtcaggctga
      361 tgcgaccgtc gctggcggct agcagcgacc gcgcgatcgg caggccgagc ccggtcccgc
      421 cctgggctct ccgactggtg aaaaagggct cgaacaggcg ctcgcggtcg gcctcggcga
      481 cgccgggccc atcgtcggtg acggtcagga cgacagcccg ctctcccgcc gcgcccgaga
      541 tgatcacctt gccggccccg gcctgacggc tgttgaccag cagcacgccc agcaccgtct
      601 cgatcgcctc ggacggcacg ccgaccatcg gcagatcacg gggcagggcg acatcaaccc
      661 tcgggttgtc gtccaggctg tcgaccaccc gtcggacaga cccttccaca tccgccgccg
      721 cgcccgcttc cggtcgcgcc atgtccgccc gcgccaggtc gagcaggcgg gtgaccagct
      781 gcgtcaggcg ggcattgtcg gcggaaatgt tggcgaggaa ccggctgcgc tcctcggggc
      841 tcatggccgc gccatggtcc tgcagcaact cgaccgcccc gccgatcccg gccagaggcg
      901 tcttgaactc atggctgacc gcggcggcga agtcgcgcag atagcgcgag cggcgggcga
      961 tcgcctcggc catcgcccgg aaatcctcgt agagctggcg gatctcgacg gccgcggtct
     1021 gcggcgtctg cggaatgtcg ccctggccgg ccgccacgtc gcgggaagcg cggctcaggg
     1081 cgacgatcgg tcgggtgatg ccgcgcgaga ccaggcccga cagcaggacc aggatcagga
     1141 tcagccccgc cagaccgatc aggaacttgc cgcggtcttc atagaggccg ctgaacagcg
     1201 cccgcggcga gcgggacaac agcagcacgc cctccacccg gccattgacc aggatcggcc
     1261 gggcgtgatg caggcgaatg cccgaggcgc ggctcagcca ctcgaacgaa tagcgtgggt
     1321 gatagtcgga ctgcctgcgc agcacggtgc gcgtctcgcc ccgcaaggct gccgccacct
     1381 ccggcaaccg gcgcaggctg ccgcccaggc ccagtccctg gaccaccgtg ccgttgcggt
     1441 cgagcagcac caccgaggcc agggtgacgc gggtcgtctc gtcgatcacc ggccgcaggg
     1501 ctggcagcac cgccagcgcc gccggatcgg ccg
//
LOCUS       DAORNX010000610         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57205_length_1532_cov_8.787407, whole genome shotgun sequence.
ACCESSION   DAORNX010000610 DAORNX010000000
VERSION     DAORNX010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57205_length_1532_cov_8.787407"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PLO65_17225"
     CDS             complement(<1..566)
                     /locus_tag="PLO65_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD30038.1"
                     /translation="MRSLRSLLVLLAAMALVACAASVERYPTSADYPAGTMRRVAIQA
                     AGHPWRLSALETPRAAPWKVVVVTGTPSWSEYWAPTMAALPAGFTMVVADRPGFALSE
                     PQAVVGSISDQAEALSALLAADPGQKVLLVGQSYGAPIAALMAARYPDRVQALVLMSP
                     FFGERGATARRLTGLGGMVRPLLPRDLK"
     gene            complement(648..>1532)
                     /locus_tag="PLO65_17230"
     CDS             complement(648..>1532)
                     /locus_tag="PLO65_17230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRD30039.1"
                     /translation="LGFITALTQPTPEDLAKEIQRTKDMTDKPFGVNLTILPAIKPPP
                     YAEYRAAIIESGIKIVETAGYKPQEHVDHFKQHGIKIIHKCTAVRHALSAERMGVDAI
                     SIDGFECAGHPGEDDIPGLVLIPAAADKVKIPMLASGGFGDARGLVAALALGADGINM
                     GTRFCVTKEAPIPDAFKQQMVANDERQTDLIFRTLHNTARVMRNAVSQQVVEIERRGG
                     AKFEDVQALVAGTRGRDALANGDTDGGIWSAGMIQGLIHDIPSCKELVERIVADAETL
                     IRGRLASMVVSPARVAAE"
BASE COUNT          213 a          520 c          556 g          243 t
ORIGIN      
        1 ttcttcaggt cacgtggcag caggggccgg accatgccgc ccaggccggt cagccgccgg
       61 gccgtcgcgc cgcgctcgcc gaagaacgga ctcatcagca ccagcgcctg gacccggtcc
      121 ggatagcgcg cggccatcag tgcggcgatc ggcgcgccgt aggactggcc gaccagaagg
      181 accttctggc ccggatcggc ggcgagcagg gccgacaggg cctcggcctg gtcgctgatc
      241 gaaccgacca ccgcctgcgg ttcgctgagg gcgaagccgg gccggtccgc gaccaccatg
      301 gtgaagccgg ccggcagggc cgccatggtc ggcgcccagt attcggacca ggatggggtg
      361 ccggtgacga ccaccacctt ccacggcgcc gcgcggggcg tttcgagggc cgacagccgc
      421 cagggatggc ccgcggcctg gatcgcgacc cgccgcatgg ttccggccgg atagtcggcc
      481 gaggtcggat agcgctcgac gctggcggcg caggcgacca gcgccatggc cgccagcagg
      541 acaagcaggg atcgcaggct tcgcacgagg tcaggctcct tcgaacgccc agcctaggcg
      601 tccgaggggc cggacagaag ttaagccttg gtcatgcgcc gacggcgcta ttccgccgcc
      661 acccgggccg ggctgacgac catgctggcc agccggccgc ggatcagcgt ctcggcgtcg
      721 gcgacgatcc gttcgaccag ctccttgcag ctggggatgt cgtggatcag gccctggatc
      781 atgccggccg accagatgcc gccgtcggtg tcgccattgg ccagggcgtc gcggccgcgc
      841 gtgccggcga ccagcgcctg aacgtcctcg aacttggctc ccccgcggcg ttcgatctcg
      901 accacctgct ggctgaccgc gttgcgcatc acccgggcgg tgttgtgcaa ggtgcggaag
      961 atcaggtcgg tctggcgctc gtcgttggcg accatctgct gcttgaaggc gtcggggatc
     1021 ggcgcctcct tggtgacgca gaagcgggtg cccatgttga tgccgtcggc gccgagcgcc
     1081 agggccgcga ccaggccgcg cgcgtcgccg aagccgcccg aggccagcat cgggatcttc
     1141 accttgtcgg ccgcggccgg gatcagcacc aggccgggga tgtcgtcctc gcccgggtgg
     1201 cccgcgcatt cgaagccgtc gatcgagatg gcgtccacgc ccattcgctc cgccgacagg
     1261 gcgtgccgga cggccgtgca cttgtggatg atcttgatgc cgtgctgctt gaagtggtcg
     1321 acatgctcct gcggcttgta gccggcggtc tcgacgatct tgatcccgct ctcgatgatc
     1381 gcggcgcggt actcggcata gggcggcggc ttgatcgccg gcaggatggt caggttcacg
     1441 ccgaacggct tgtcggtcat gtccttggtg cgctggattt ccttggccag gtcttccggc
     1501 gtcggctggg tcagggcggt gatgaagccc ag
//
LOCUS       DAORNX010000611         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57442_length_1527_cov_4.440897, whole genome shotgun sequence.
ACCESSION   DAORNX010000611 DAORNX010000000
VERSION     DAORNX010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57442_length_1527_cov_4.440897"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLO65_17235"
     CDS             complement(<1..1073)
                     /locus_tag="PLO65_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30040.1"
                     /translation="MSQPPIDRLNAADVAAVLERFGGHDLMGRGAVNIISLAAIRDRS
                     GDRWPRKRPDVWAYVERKLAEHLTYQDLAQRVGEADYLIAMTSEEGVAAQAVALKVLK
                     EVLMHFLGAAEPRDMAVRAVTDLKDGEVSCAPLDPVFILQTRAREPAPATPAVRPDVD
                     PDELRKRNPFSFHAAGGMALRIDFAVEDLLSLKHGVTAALRIEPTVTDVATGRVIPAR
                     AFGRLPDADLLKIDQATLDFAALFLPRVVGQGQPALIAPVSFRTMATSRGRGLLINCA
                     GAAAGRVKASMMLELVDIDRGAPLARLIEVVGLLKSVCRGVFARVQPARDAMAPLTGV
                     RLMGVTLDAADLPGPDGRIATQIL"
     gene            1186..>1527
                     /gene="crcB"
                     /locus_tag="PLO65_17240"
     CDS             1186..>1527
                     /gene="crcB"
                     /locus_tag="PLO65_17240"
                     /inference="COORDINATES: protein motif:HMM:NF010791.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRD30041.1"
                     /translation="MDKLLLVAAGGATGAMLRYLTGIGATRALGTGWPWGTLTVNVVG
                     GLAMGLLVGVLAQRGGADQERWRLLLGVGVLGGFTTFSAFSLDAMLLWQRGDAVGFLL
                     YGLEIVPMTIGG"
BASE COUNT          199 a          532 c          565 g          231 t
ORIGIN      
        1 agaatctggg tggcgatgcg gccatcgggg ccgggcaggt cggcggcgtc gagcgtcacc
       61 cccatcagcc gcaccccggt cagcggcgcc atcgcgtccc gggccggctg gacccgggcg
      121 aacacgccgc gacagactga cttgagcagc ccgaccacct cgatcagccg ggccagcggc
      181 gcgccgcggt cgatgtcgac cagttccagc atcatgctgg ccttgacccg cccggcggcg
      241 gcgccggcgc agttgatcag caggccgcgg cccctgctgg tggccatggt gcggaacgac
      301 acgggcgcga tcagcgccgg ctggccctgg ccgacgaccc gcggcaggaa cagggcggcg
      361 aagtccaggg tcgcctggtc gatcttcagc aggtccgcgt ccggcagcct gccgaaggcg
      421 cgggcgggga tcacccggcc ggtggcgaca tcggtgacgg tcggctcgat gcgcagggcc
      481 gcggtgacgc cgtgcttgag gctcagcagg tcctcgacgg cgaaatcgat ccgcagggcc
      541 atgccccccg cggcgtggaa gctgaagggg ttgcgtttgc gcagttcgtc ggggtcgaca
      601 tccggccgca ccgccggcgt ggccggggcg ggctcgcggg cgcgggtctg caggatgaac
      661 accgggtcga ggggcgcgca ggacacctcg ccatccttca ggtctgtgac cgcgcgcacg
      721 gccatgtcgc gcggctccgc ggcgcccagg aaatgcatca gcacctcctt cagcaccttc
      781 agcgccacgg cctgggccgc cacgccttcc tcgctggtca tggcgatcag gtagtcggct
      841 tccccgaccc gctgggccag gtcctggtag gtcaggtgct cggccagctt gcgctcgaca
      901 taggcccata cgtccggccg cttgcgcggc cagcggtcgc cgctgcgatc gcggatcgcc
      961 gccaggctga tgatgttgac ggcccccctg cccatgaggt cgtgaccgcc gaaacgctcg
     1021 agcacggcgg cgacatcggc ggcattgaga cggtcgatcg gaggctggct catggacgcg
     1081 gtcgatcttc ggctcagggt tccccaattt ggggcctcgc gcttaacggg cggtttaggc
     1141 gccgacccgg ttcgacacga tcgtccgccc gtcgcataag ggggcatgga caagttgtta
     1201 ctcgtcgctg ccggcggcgc cacgggcgcg atgctgcgct acctgaccgg catcggggcg
     1261 acccgcgccc tcgggaccgg ctggccctgg ggaacgctga cggtcaacgt cgtcggtggc
     1321 ctggccatgg ggctgctggt cggggtgctg gcccagcgcg gcggggcgga ccaggagcgc
     1381 tggcggctgc tgctcggcgt cggcgtgctc ggcggcttca ccaccttctc cgccttctcg
     1441 ctcgacgcca tgctgctgtg gcagcgcggc gacgcggtcg gcttcctgct ctacgggctg
     1501 gagatcgtgc cgatgaccat cggcggc
//
LOCUS       DAORNX010000612         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57488_length_1526_cov_3.169273, whole genome shotgun sequence.
ACCESSION   DAORNX010000612 DAORNX010000000
VERSION     DAORNX010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57488_length_1526_cov_3.169273"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>650)
                     /locus_tag="PLO65_17245"
     CDS             complement(102..>650)
                     /locus_tag="PLO65_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HRD30042.1"
                     /translation="PAVAAPPWNGAYDELPGGSYQRSCRDITAFNGRLYARCQTPGGG
                     FVDSEVYYRACAQGRVENVDGRLQCEGWSGGGGYGGPGGPGPGPGGYGGGGPTGLIAY
                     ENPDYRGMRLEVSDSIPDLYASGLDDQITSVRVLRGSWQLCTGRDFNGQCWTVDADTP
                     NLKVLGANDKVSSIRRMPGRGR"
     assembly_gap    651..660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(776..>1526)
                     /locus_tag="PLO65_17250"
     CDS             complement(776..>1526)
                     /locus_tag="PLO65_17250"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRD30043.1"
                     /translation="LLAAFSLMAPDLLAAARPRSAEAAADRLTVLQFNLWGRNRDPAG
                     TARWILDTDPDIIVLEEGVNGSGGIVKALARRYPHRTTCAEPRPCSTIILAKRRPVSE
                     GGLASSAALPGAWATFADRHGAPFTVVGVHYTWPWPAGPQQQQTRRLAAALAAFPKDR
                     LIVAGDFNSTPWSFSLRRQDRMFGLDRRTRALASWPAGDVSRLHVAAPFPLLPIDQVY
                     AGRGWRTVAVRRGPLLGSDHYPVLVTLAVAP"
BASE COUNT          220 a          527 c          575 g          194 t
ORIGIN      
        1 acacgccccg gctcagcgcc ggggctgctc aggatgacgt gactccgcgc acgtcatggt
       61 gagcagcccg cgcagcgggc gtgtcgaacc acgcgacctg cctaccgccc ccgacccggc
      121 atccggcgga tcgaactgac cttgtcgttg gcgcccagca ccttgaggtt gggggtgtcg
      181 gcgtcgacgg tccagcactg gccgttgaag tcgcggccgg tgcacagctg ccagctgccg
      241 cgcagcaccc gcaccgacgt gatctggtcg tctaggccgg aggcgtagag gtcggggatc
      301 gagtcggaca cctccagccg catgccgcga tagtcggggt tttcgtaggc gatcagcccg
      361 gtcggtccgc cgcccccgta gccgccgggg ccaggaccgg ggccgcccgg gccgccatag
      421 ccgccgccgc ccgaccatcc ctcgcactgc aggcggccgt cgacgttctc cacccgcccc
      481 tgggcgcagg cgcggtagta gacctcgctg tcgacgaagc cgccgccagg cgtctggcac
      541 cgggcgtaga gccggccgtt gaaggcggtg atgtcgcggc agctcctctg gtaggagccg
      601 ccgggcagtt catcgtaggc gccgttccag ggcggcgccg ccacggcggg nnnnnnnnnn
      661 cgaccaggat ggtgccggcg gcgaacgcgg ccgcgacagg atggatggac atggtggctc
      721 ccccgggaat gtttcgcagc ggccaatgta ggggcgcggc gcggaggccg tccagtcagg
      781 gcgcgacggc cagggtgacc agcaccgggt agtgatccga tcccagcagc ggaccgcgcc
      841 gcaccgccac cgtccgccag ccgcgaccgg cgtagacctg atcgatgggc agcaggggga
      901 acggggcggc gacatgcagc ctggacacgt cgcccgccgg ccagctggcc agcgcccgcg
      961 tgcgtctatc aaggccgaac atccggtcct ggcggcgcag cgagaatgac cagggcgtgg
     1021 agttgaagtc gccggcgaca atcagccggt ccttgggaaa cgccgccagc gccgcggcca
     1081 gccgccgcgt ctgctgctgc tgcgggccgg ccggccaggg ccaggtgtag tgaacgccga
     1141 ccacggtgaa gggcgcgcca tgcctgtcgg caaaggtggc ccaggctccg ggcagggcgg
     1201 ccgaggatgc cagccccccc tcggacaccg gccgacgctt ggccaggatt attgtcgagc
     1261 agggccgcgg ttcggcgcag gtggtgcggt gggggtaacg ccgcgcgagc gccttgacga
     1321 tgccgcccga gccgttgacg ccctcttcaa ggacgatgat gtccgggtcg gtgtcgagga
     1381 tccagcgggc cgtgccggcg ggatcgcggt tgcggcccca caggttgaac tgcagcacgg
     1441 tgagcctgtc ggcggcggcc tcggccgacc ggggccgggc ggccgccagc aggtcgggcg
     1501 ccatcaggct gaacgcggcc agcagg
//
LOCUS       DAORNX010000613         1521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57704_length_1521_cov_6.613233, whole genome shotgun sequence.
ACCESSION   DAORNX010000613 DAORNX010000000
VERSION     DAORNX010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57704_length_1521_cov_6.613233"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLO65_17255"
     CDS             complement(<1..299)
                     /locus_tag="PLO65_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30044.1"
                     /translation="MSLTRILTAAAVSALFAAPALAAPDPATTPDPMQPSDQMQSTDT
                     RPMNPPSADQAATPASATVVDPSAPAVVLQDSPTPPDQAWRLKVGDPSVVTNGPVP"
     gene            complement(403..756)
                     /locus_tag="PLO65_17260"
     CDS             complement(403..756)
                     /locus_tag="PLO65_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRD30045.1"
                     /translation="MFALLWPRLGPVLLLLASNVFMTFAWYGHLKHKGAALPLVILTS
                     WMIALPEYALAVPANRLGSAVWTTAQLKTMQEVITLLVFAGFSVLYLGEPLKLSTLLG
                     FALIAAGAGLVFLGR"
     gene            1034..1372
                     /locus_tag="PLO65_17265"
     CDS             1034..1372
                     /locus_tag="PLO65_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD30046.1"
                     /translation="MKLIIAVVKPFKLDEVREALVGAGVEGLTVSEVKGYGRQKGQTE
                     IYRGAEYQVNFVPKVKLEAVVDDAAAAKAVEAIKAAAATGKIGDGKIFVLNVEDAVRI
                     RTGETGSAAL"
BASE COUNT          254 a          507 c          556 g          204 t
ORIGIN      
        1 gggaccggcc cgttggtcac caccgacgga tcgccgacct tgagccgcca ggcctggtcc
       61 ggcggggtcg gcgagtcctg caggaccacc gccggggccg acggatcaac gacagtggcc
      121 gacgccggtg tcgcggcctg gtcggccgac ggcgggttca tcggtcgggt gtccgtcgac
      181 tgcatctggt cggacggctg catgggatcg ggggtcgtgg cgggatcagg cgcggcgagg
      241 gccggggcgg cgaacagggc cgaaacggct gcggcggtca ggattcgggt cagggacatc
      301 gctttgaacc tcatgtcgcg acgcggctgc gtcgtccccc gacaggcgaa ctacgcgcca
      361 accccgagtc gggttccatc gccgcgatga ccccgtgccg gctcagcgcc cgaggaacac
      421 cagtcccgcg ccggccgcga tcagggcgaa gcccagcagg gtcgagagtt tcaacggctc
      481 gccgaggtaa aggaccgaga agcccgcgaa caccagcagg gtgatgacct cctgcatcgt
      541 cttgagctgg gcggtggtcc agaccgccga tccgagccgg ttggccggaa cggcgagggc
      601 gtactccggc agggcgatca tccagctggt caggatcacc agcggcagcg ccgcgccctt
      661 gtgcttcagg tgaccgtacc aggcgaatgt catgaagacg ttggaggcca ggagcagcag
      721 caccggcccc agcctcggcc aaagcaacgc gaacatcgat tgtctcccct gccgcccgcc
      781 cgtcgggcgt gaatcccggc cgcccggccg tcgtggcgcc tgtttttgag gcgccgccgc
      841 ccctgcccgc ccagccattg ggcgaaccgg cggtttcccg gcccttcatt gtgcagcgca
      901 cacgtccggg caagccccgc cgctagcctg ccggacggat cggcggatcg cgaaccagcg
      961 cgtcgcgacg ggaccggtcg gcagggaaat tcagggacgc aggccgggca accggattgc
     1021 gggagcaagg cggatgaaac tgatcattgc ggtggtcaag cccttcaagc tcgacgaggt
     1081 gcgcgaagcc ctcgtcggcg ccggcgtcga gggtctgacg gtctccgaag tcaagggcta
     1141 cggccggcag aagggccaga cggaaatcta ccggggcgcg gagtaccagg tgaacttcgt
     1201 gccgaaggtg aagctggaag ccgtcgtcga cgacgccgcg gccgccaagg cggtcgaggc
     1261 gatcaaggcc gccgcggcca ccggcaagat cggcgacggc aagatcttcg tcctgaacgt
     1321 cgaggacgcg gtgcgcatcc ggaccggcga aaccggctcg gcggcgctct gaaacatcat
     1381 cgggacaagg ggatagcaac gatgaaaccg acttttctcg gacggctggc ggcgctgacc
     1441 gccgtcgccg ttctcgccgg ggcgccgctg gcggctccgg tgttcgcgca ggaggcggcc
     1501 ccggccgcca ccgcggctgc g
//
LOCUS       DAORNX010000614         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_57742_length_1520_cov_4.900341, whole genome shotgun sequence.
ACCESSION   DAORNX010000614 DAORNX010000000
VERSION     DAORNX010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57742_length_1520_cov_4.900341"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..318)
                     /locus_tag="PLO65_17270"
     CDS             complement(109..318)
                     /locus_tag="PLO65_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HRD30047.1"
                     /translation="MLEEPAGPRGHRGEALSLALREDLELYSVDDLEERVGLLQAEIA
                     RTQAQIERKRASKSAADAFFKFGEN"
     gene            402..1379
                     /locus_tag="PLO65_17275"
     CDS             402..1379
                     /locus_tag="PLO65_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRD30048.1"
                     /translation="MAEPSMTAIAIEGGKGPAEALRPVSVARPVPGDGEILIRVRAAG
                     VNRPDLLQRLGFYPPPPGAPATLGLEVAGEVAVGAGRWKEGDRVTALLGGGGYAEYAV
                     VDARHALPIPAGLDFAHAAGLPETVFTVFANVFEHGGLKAGETFMVHGATSGIGVTAI
                     QMARAAGAKVIATGRGADKAAQALALGADVAVDTTTQDFAEAATAEGGVDVILDMVGA
                     SYFPKNLQALKTGGRIVYIASLGGGMLEVPVLAIMQKRAVITGSTLRPRSSDEKARLA
                     AEIERVVWPWIEAGKVKAVVDRTFPLADAAGAHAFLEAGAHVGKVVLEA"
BASE COUNT          221 a          548 c          531 g          220 t
ORIGIN      
        1 tcacgggcgc gccgagttca ttcatggaca agatcgcccc tcacaaaacg cgcggatcaa
       61 ctttcccgca ttgccccgat ggctgcaacc gccgtgccaa gccggcggtc agttttcgcc
      121 aaacttgaaa aaggcgtccg cggccgactt gctggcccgc ttccgctcga tctgggcctg
      181 ggtgcgggcg atttccgcct gcagcagacc gacgcgctcc tccagatcgt ccacgctgta
      241 gagttcgaga tcctcgcgca gggcgaggga cagcgcctca cccctgtgtc cgcgcggacc
      301 ggccggttcc tccagcatcg cgaggcctcc ctatcgccat cgacgccttt cacgatatca
      361 gacgcgcgcg gacgataaca cgcaagtcca ggagcgagac catggccgag ccgagcatga
      421 ccgccatcgc catcgagggc ggcaagggcc ccgccgaggc gctgaggccc gtttcggtcg
      481 cgcggccggt cccgggggac ggcgaaatcc tgatccgggt cagggccgcc ggggtcaatc
      541 gtcccgatct gctccagcgc ctgggcttct atccgccgcc gcccggcgcg ccggccaccc
      601 tggggctgga ggtcgccggc gaggtggccg tcggcgccgg gcgatggaag gaaggcgacc
      661 gggtcaccgc cctgctcggc ggcggcggct acgctgaata cgcggtggtc gacgcccgcc
      721 acgccctgcc cattcctgcc gggctcgact ttgcccacgc cgccggcctg cccgagaccg
      781 tgttcacggt gttcgccaat gtgttcgagc acggcgggct gaaggccggg gagaccttca
      841 tggtccacgg cgccacctcc ggcatcgggg tcaccgcgat ccagatggcc agggccgcgg
      901 gcgccaaggt catcgctacc ggccgcggcg cggacaaggc ggcccaggcc ctggcgctgg
      961 gcgccgacgt ggccgtcgac accacgaccc aggacttcgc cgaggcggcc acggccgagg
     1021 gcggcgtgga cgtgatcctc gacatggtcg gggcgtcgta tttccccaag aacctgcagg
     1081 cgctgaagac cggtggccgc atcgtctaca tcgcgtccct gggcgggggc atgctcgagg
     1141 ttccggtgct ggccatcatg cagaagcggg cagtgatcac cggctcgacc ctgcggcccc
     1201 gctccagcga cgagaaggcc cggctggcgg ccgagatcga acgggtcgtc tggccgtgga
     1261 tcgaggccgg caaggtcaag gcggtggtcg accgcacatt cccgctggcc gatgccgccg
     1321 gggcccacgc cttccttgag gccggagccc atgtcggcaa ggtcgtgctg gaggcctgag
     1381 gtgagcgcgc catccccgcc cgtcgatccg cgcgtcctgg tccagcaggc catcgagggc
     1441 ctgcgccggg gcgatccggc cgcggcccgc gaccgcttcc agcaggtgat cgacgccggc
     1501 gtggccgacg cctcggtctg
//
LOCUS       DAORNX010000615         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_58083_length_1512_cov_5.381606, whole genome shotgun sequence.
ACCESSION   DAORNX010000615 DAORNX010000000
VERSION     DAORNX010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58083_length_1512_cov_5.381606"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PLO65_17280"
     CDS             <1..743
                     /locus_tag="PLO65_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRD30049.1"
                     /translation="TFRTLDLGGDKLLPYMELEREENPALGWRAVRMGLDRPALLRLQ
                     LRALIWAAHGRPLRIMFPLVASVDEFRAARAFVDAELAWAQRRGRAAPSRLDVGAMIE
                     APSLLWHLDALLPMTDFVSVGTNDLMQYLFAADRGNPRVADRYDPLSPPALRALKQIQ
                     DACAETGTPVSVCGEMAGRPLEAFALVALGFDRLSMPPAGIGPVKQMVLSLDREAAHR
                     GVLQLIRGATGSVRNEIETLARKLYVAV"
     gene            937..>1512
                     /locus_tag="PLO65_17285"
     CDS             937..>1512
                     /locus_tag="PLO65_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD30050.1"
                     /translation="MDSADHLGPTEEPSLLYGPDIGAALKAAREFRGLSLQDVSDATR
                     IRRTYLAALEDMRLDELPSRPFTLGYIKAYARHLDLDPEEAVLRFKDSSPDPDEPLRA
                     PVGVRRERDPRMGAIVAGGVLIVSAIVLWNIAQRAITEDAPPPATAPESVASALPAAP
                     AGPMPLGAPLPAPVEASTPEVYKTPGLEASAA"
BASE COUNT          204 a          549 c          516 g          243 t
ORIGIN      
        1 tgaccttccg cacgctcgac ctggggggcg acaagctgct gccctacatg gagctcgagc
       61 gggaggagaa cccggccctg ggctggcgcg ccgtgcgcat gggcctggac cggcccgccc
      121 tgctgcggct gcagctgcgg gcgctgatct gggccgcgca cggtcgcccc ctgcggatca
      181 tgttcccgct cgtcgccagc gtggacgagt tccgcgccgc ccgggccttc gtcgacgccg
      241 agctggcctg ggcccagcgc cgcggccggg ccgctccgtc gcggctggat gtcggggcca
      301 tgatcgaggc gccgtccctg ctctggcacc tggacgccct gctgccgatg accgacttcg
      361 tctcggtcgg caccaacgac ctgatgcagt atctgttcgc cgccgaccgc gggaacccgc
      421 gcgtggccga ccgctacgac cccctgtcgc cgccggcgct gcgggcgctc aagcagatcc
      481 aggacgcctg tgccgaaacg ggaacgccgg tctcggtctg tggcgaaatg gccggccggc
      541 cgctggaggc tttcgccctg gtggcgctcg gcttcgaccg gctgtcgatg ccgccggccg
      601 gcatcggccc ggtcaagcag atggtgctgt cgctcgatcg cgaggccgcc caccgcggcg
      661 tgctgcagct gatccggggc gccaccggct cggtgcgcaa cgaaatcgag accctggccc
      721 ggaaattgta tgtcgccgtc tgacggttaa cgggcagcga cggattagca cattgaattc
      781 cggacgttat tttgatatcc acaactctgc gttaagaatt cccggccgaa gggaagaacc
      841 gtgcgtcgcg ctcgcgcgcg ccgcgagttt gtgggggcgt cggtagccat gccgcttgat
      901 tctggcgggg ttacgcaact gtatcgttca ggggatatgg actcggccga tcatcttgga
      961 ccgaccgagg agccgtccct gctctatgga cccgatatcg gcgccgccct gaaggccgcg
     1021 cgcgagttcc gcggcctgtc cctgcaggac gtttcggacg ccacccggat tcgccgcacc
     1081 tacctggccg cgcttgagga catgcgcctg gacgagctgc cctcgcggcc gttcacactc
     1141 ggctacatca aggcctacgc caggcatctg gacctcgacc ccgaggaagc ggtcctgcgc
     1201 ttcaaggatt cctctcccga tccggacgag ccgctgcgcg cgccggtcgg cgtgcgtcgc
     1261 gaacgcgacc cgcgtatggg cgccatcgtc gccggcgggg tgctgatcgt ctcggcgatc
     1321 gtgctgtgga acatcgccca gcgcgccatc accgaggacg cgccgccgcc cgcaaccgcg
     1381 cctgaaagcg tggccagcgc cctgccggcc gctccggccg gtccgatgcc gctgggcgcg
     1441 ccgctgcccg cgccggtcga ggcctccacg ccggaggtct acaagacccc gggactcgag
     1501 gcctccgccg cc
//
LOCUS       DAORNX010000616         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5436
            NODE_58307_length_1507_cov_7.378788, whole genome shotgun sequence.
ACCESSION   DAORNX010000616 DAORNX010000000
VERSION     DAORNX010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867653
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:56:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,460
            CDSs (total)                      :: 3,421
            Genes (coding)                    :: 3,394
            CDSs (with protein)               :: 3,394
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 18 of 27
            Pseudo Genes (internal stop)      :: 1 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58307_length_1507_cov_7.378788"
                     /isolate="AMR_MDS_5436"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLO65_17290"
     CDS             <1..625
                     /locus_tag="PLO65_17290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRD30051.1"
                     /translation="DVSAAVAEARARAAEAQTLEALAAAIAAFDGCGLKFEGAKQAVF
                     ARGNPQGPVMAIGEGPGGEEDARGEPFVGKAGQLLDRMFEAAGLTDRVFITNTVFWRP
                     PGNRTPTAAEQAVCAPFLHRAIQLVQPRMLLLVGGASAKSVLQREEGILTLRGRWFEW
                     HGDDGAVLPALPTLHPAFLLRQPAAKKKAWADLLTLTERLDRPGRGE"
     gene            622..1032
                     /locus_tag="PLO65_17295"
     CDS             622..1032
                     /locus_tag="PLO65_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30052.1"
                     /translation="MTIGAAITVLVVLDPDFGDHLVNVPSDQPVWIVESLANTPAARR
                     IWSDGVERTGGVTLFKSSGDTRERVFRRQLGVIDLHHGPYSSDESYTGIKVIGASLTG
                     EIEASLLASGFAPPTATADGFKATRSRVEAQRLR"
     gene            complement(1277..>1507)
                     /locus_tag="PLO65_17300"
     CDS             complement(1277..>1507)
                     /locus_tag="PLO65_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD30053.1"
                     /translation="PADAQWCLEQGCDFVLIGRGAILHHDWPKLAAADPAFRPIALPV
                     TREHLHREGLSDRFVTYMQAWKGFVAEEMAEA"
BASE COUNT          252 a          500 c          516 g          239 t
ORIGIN      
        1 cgacgtttcc gccgccgtgg ccgaggccag ggcccgggcc gcagaggcgc agacgctcga
       61 ggcgctggcc gccgccatcg ccgccttcga cggctgcggt ctgaaattcg agggcgcgaa
      121 acaggcggtc ttcgcgcgcg gcaatccgca aggaccggtg atggccatcg gcgaaggccc
      181 cggcggcgaa gaggacgccc gcggcgagcc gttcgtcggc aaggccggcc agctgctcga
      241 ccggatgttc gaagcggccg gcctgacgga ccgggtgttc atcaccaaca cggtgttctg
      301 gcgcccgccc ggcaaccgta cgcccaccgc ggccgaacag gccgtctgcg cgcccttcct
      361 gcaccgggcg atccagctgg tgcagccaag gatgctgctg ctggtcggcg gcgcctcggc
      421 caagtcggtg ctgcagcggg aggaaggcat cctcacactg cgcggtcgct ggttcgaatg
      481 gcatggcgac gacggggcgg tgctgccggc cctgccgacg ctgcatccgg cgttcctgct
      541 caggcagccc gccgcgaaga agaaggcctg ggcggatttg ctgacgctga ccgagcggct
      601 ggatcgcccg ggacggggtg agtgaccatc ggcgccgcga taaccgtgct cgtcgttctt
      661 gatcccgatt tcggtgatca cctggtcaat gttccgtcgg atcaacccgt atggatcgtc
      721 gaatccctcg cgaacacgcc ggcggcaaga aggatatgga gcgacggggt ggaacggacg
      781 ggaggtgtga ccctgttcaa atcgtccggc gacacccgtg aacgggtatt caggcgccag
      841 ctgggcgtta tcgacctcca tcacggaccg tactcaagtg acgagtccta cacaggcatc
      901 aaggtgatag gcgcctctct gacgggggaa atcgaagcca gtctactggc ttcgggtttc
      961 gcgccaccca cagccacagc tgacggtttc aaggcgacca ggagccgggt ggaagcgcaa
     1021 cgcttgcgtt gacgccctcc cttcatctca tggcgagagc ttcgccggac ggatcaggtc
     1081 actcaaggct ccgcaggacc gctgcgcggc gggccgcagg cccggccttg actgacctga
     1141 cccgtccggc acgctggctt gcatgagcct aagggccacc tctccccttt gatcggggag
     1201 atcatcccaa acccccaaat ccgtcatggt ccggacaagc cgggccatga cagttgtcgt
     1261 gtggcgttcg ctgaagctaa gcctcggcca tctcctccgc cacgaagcct ttccaggcct
     1321 gcatgtaggt gacgaaacgg tcgctcagcc cctcgcggtg gaggtgttcg cgggtcaccg
     1381 gcagggcgat ggggcggaag gccggatcgg ccgccgcgag cttcggccag tcgtggtgca
     1441 ggatcgcgcc gcggccgatc aggacgaagt cgcagccctg ctccaggcac cactgcgcgt
     1501 cggccgg
//
